LOCUS       DAEMGG010000001        79148 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEMGG010000001 DAEMGG010000000
VERSION     DAEMGG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 79148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.001"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            414..1247
                     /locus_tag="KPK24_000001"
     CDS             414..1247
                     /locus_tag="KPK24_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG2297725.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1340..2272
                     /locus_tag="KPK24_000002"
     CDS             1340..2272
                     /locus_tag="KPK24_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2297726.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEVLKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2324..2806
                     /locus_tag="KPK24_000003"
     CDS             2324..2806
                     /locus_tag="KPK24_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2297727.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQESMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2818..3687
                     /locus_tag="KPK24_000004"
     CDS             2818..3687
                     /locus_tag="KPK24_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG2297728.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIRAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3730..4485
                     /locus_tag="KPK24_000005"
     CDS             3730..4485
                     /locus_tag="KPK24_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG2297729.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSITKIKGLKDKTLENKLNNELLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSIYIKTISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4527..5348
                     /locus_tag="KPK24_000006"
     CDS             4527..5348
                     /locus_tag="KPK24_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HBG2297730.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5353..6114
                     /locus_tag="KPK24_000007"
     CDS             5353..6114
                     /locus_tag="KPK24_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG2297731.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSDCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6275..6532
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6645..6884
                     /locus_tag="KPK24_000008"
     CDS             6645..6884
                     /locus_tag="KPK24_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG2297732.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7188..8183
                     /gene="ilvC"
                     /locus_tag="KPK24_000009"
     CDS             7188..8183
                     /gene="ilvC"
                     /locus_tag="KPK24_000009"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG2297733.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDHT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8236..9924
                     /gene="ilvB"
                     /locus_tag="KPK24_000010"
     CDS             8236..9924
                     /gene="ilvB"
                     /locus_tag="KPK24_000010"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG2297734.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSIEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(10285..11415)
                     /locus_tag="KPK24_000011"
     CDS             complement(10285..11415)
                     /locus_tag="KPK24_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2297735.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKDDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(11655..12161)
                     /locus_tag="KPK24_000012"
     CDS             complement(11655..12161)
                     /locus_tag="KPK24_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG2297736.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            complement(12424..14013)
                     /locus_tag="KPK24_000013"
     CDS             complement(12424..14013)
                     /locus_tag="KPK24_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG2297737.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IVFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            complement(14037..14600)
                     /locus_tag="KPK24_000014"
     CDS             complement(14037..14600)
                     /locus_tag="KPK24_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2297738.1"
                     /translation="MSILSDRLKFLRKEKGVMQKEIANYLNITTSAYGFYEQGKRTPT
                     PEMLSSLAEYFGTTVDYLIGRYDNKASNISSKTSCNNTLFQKRLKELRAEKNMTQEDV
                     ANKLNLTKSAYGYYEQGKTVPDAYMLSSLAEIFNVTTDYLLGRSIVKNDIDTVAAHRV
                     NPHKDLPEEAQEQLNDYIEFLINKYKK"
     gene            14753..14974
                     /locus_tag="KPK24_000015"
     CDS             14753..14974
                     /locus_tag="KPK24_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2297739.1"
                     /translation="MNNLKELRTERNLKQKDVALKVGITTSYYGMIESGTRVPSLAIA
                     IKLSKCFGVSIEKIFLNTNTTRCCLKERS"
     gene            14977..15792
                     /locus_tag="KPK24_000016"
     CDS             14977..15792
                     /locus_tag="KPK24_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HBG2297740.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKREKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFEEMEQVLKNEQPKLPTTYKEALQHLIEQVEVNEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHS
                     ETIDITRSDGMTDVKMTTKWTQKGRLFLYDLLKVNNILPDIEKEYSYQTSMLC"
     gene            complement(15971..16150)
                     /locus_tag="KPK24_000017"
     CDS             complement(15971..16150)
                     /locus_tag="KPK24_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBG2297741.1"
                     /translation="MSTSLPKYTLRINRVLLEKIKYIAESEGRSANKEIEQIIKKHIE
                     DYEQRKGEIKINIEE"
     gene            16286..16465
                     /locus_tag="KPK24_000018"
     CDS             16286..16465
                     /locus_tag="KPK24_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297742.1"
                     /translation="MNSNKKRITVRMPEKLNEEITKKSKYLGLTKNSFILDILWKEFE
                     LLEYRSYKKEVDKHE"
     gene            16458..17249
                     /locus_tag="KPK24_000019"
     CDS             16458..17249
                     /locus_tag="KPK24_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBG2297743.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDSRNISNNFNNNKDKFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFASKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPTCIEDVLIESLKEMKDLRLQVNQANSIALEAKTEVETIKDVVSLDS
                     NSWRTNTHQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            complement(17332..17526)
                     /locus_tag="KPK24_000020"
     CDS             complement(17332..17526)
                     /locus_tag="KPK24_000020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297744.1"
                     /translation="MKFVYVKIFNNWIDITDIGSINGCNAIEYINENLDKMYSSNYVS
                     INYKDKNYCIHPSCIQIVTK"
     gene            complement(17682..17900)
                     /locus_tag="KPK24_000021"
     CDS             complement(17682..17900)
                     /locus_tag="KPK24_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2297745.1"
                     /translation="MTKFKDNIKLVRKQMNMTQKQFASLFGISERAYQYYEAGSREPN
                     LETLILISNKLNVSTDFLLGLSPNQNRN"
     gene            18013..18207
                     /locus_tag="KPK24_000022"
     CDS             18013..18207
                     /locus_tag="KPK24_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2297746.1"
                     /translation="MKINNNLKLQREKIGLTQLEVAQKAKITERSYQYYEAGERLPNI
                     RTALKIAIILNTNCEKLFNE"
     gene            18249..19043
                     /locus_tag="KPK24_000023"
     CDS             18249..19043
                     /locus_tag="KPK24_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBG2297747.1"
                     /translation="MNNLQLNNKNTITTLEIADMLEIRHWEVLRKLEGTEKTKGIIDI
                     LNDNNFVVVDYFIKSTYLDSKNESRPCYNVTKLGCDFLANKFTGEKGIIFTARYVRRF
                     NEMERVLKEQQPKLPTTYKEALQQLLVEVEEKEQLQLENQEKDKVIQLQQPKVLFADA
                     VSASNNSILVGELAKLIKQNGVDIGQNRLFTWMRENGYLIKRKGEDYNIPTQKSMNLR
                     IMEVKKRVINNPDGSTKVTRTVKITGKGQVYFVNKFKSSKQLSMLS"
     gene            19100..19309
                     /locus_tag="KPK24_000024"
     CDS             19100..19309
                     /locus_tag="KPK24_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297748.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(19319..19693)
                     /locus_tag="KPK24_000025"
     CDS             complement(19319..19693)
                     /locus_tag="KPK24_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297749.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            19769..19966
                     /locus_tag="KPK24_000026"
     CDS             19769..19966
                     /locus_tag="KPK24_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2297750.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELNIDVSGLYKLLKNKNSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            19997..20389
                     /locus_tag="KPK24_000027"
     CDS             19997..20389
                     /locus_tag="KPK24_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2297751.1"
                     /translation="MDISESITKQFSDSLKSLIEIEINKQETDRVKSQTVEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTVFMRRLVSTGLIKSLARGDGRKISRYEV
                     DDFIERNQGKNLDELLKVAERGDEIVKP"
     gene            20376..20519
                     /locus_tag="KPK24_000028"
     CDS             20376..20519
                     /locus_tag="KPK24_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297752.1"
                     /translation="MLSLNTNKNNIVTLKKDGKAIADIVFKDIKTGKKISVGVLNKKV
                     LVK"
     gene            20686..20928
                     /locus_tag="KPK24_000029"
     CDS             20686..20928
                     /locus_tag="KPK24_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297753.1"
                     /translation="MEAARLIAIGQIKQAEKEICKLQGTKNNSSLMWWEAVKFASQNI
                     LEGLEHDIELEASIEFREAMMYQEELEKDRPIDVQI"
     gene            21007..21288
                     /locus_tag="KPK24_000030"
     CDS             21007..21288
                     /locus_tag="KPK24_000030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297754.1"
                     /translation="MKTKNEIIKGLEDRLFLLRFTTVDEVDWDVKFGQISALEFCIDK
                     HRKGCTLEQFKEHLDEYKLQGNYGDYIDGFVSVLERNIREMEGEIDGSE"
     gene            21275..21913
                     /locus_tag="KPK24_000031"
     CDS             21275..21913
                     /locus_tag="KPK24_000031"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG2297755.1"
                     /translation="MEVNNIYIKLMDVRIKFNKLDIKKSGQNKFANFKYFELADFLPQ
                     ATELLQEAKLCPIVTFTNDYATLTLINGEKPTEEIIFTSPMRELQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDTFDATSGKEDYKRNDLTNSSIKASENGQIKISQNQIKRL
                     FSIGNTIGKDSDRVKSEVYYKFNKEVKDLSKQEYDQICVGYEKLQREKGIIK"
     gene            21923..22819
                     /locus_tag="KPK24_000032"
     CDS             21923..22819
                     /locus_tag="KPK24_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG2297756.1"
                     /translation="MKDRDKATIEKGNILSDGYGLSPQLVARDSWLTTGARALYFYLS
                     SFAGASGTCYPSRDIMTHELGINKDTFSKYLNELKTSGYIKVYKNKTREGRMQNNIYE
                     VVFDRSYIESHISIRCKKENKKKPCPKKADMEPCPNLPDMVQPDMEKSDTISNSIINN
                     SFKSSMYIEQAVDNSLKEFKKLYEENIGVVYPVTAEWLLEVSNEVDIRVFKRAIEICA
                     ERMNMNLSYLKGILKKWKDANITTYEQLESYKLQHENKKSKKTNSVVSKNKFANFEQT
                     FTQYSNKELDEIIKKSQKAKFK"
     gene            22839..23258
                     /gene="ssb"
                     /locus_tag="KPK24_000033"
     CDS             22839..23258
                     /gene="ssb"
                     /locus_tag="KPK24_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2297757.1"
                     /translation="MNQVVLVGRLTRDPELKYIPGTGTAVASFTIAVDRNYINKEGKR
                     DTDFIPIEVIGKSAEYCANYITKGKLVALEGNIRVDNYQTQSGEKRIFTKVSTKSVQS
                     LESKNKSSNSYKESVQDGTIGLDPQGFEIIDDDELPF"
     gene            23288..23650
                     /locus_tag="KPK24_000034"
     CDS             23288..23650
                     /locus_tag="KPK24_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297758.1"
                     /translation="MFKVERYFSGSVVDNLIEDDLTCRNYLALYCCLLGITKNGKKIY
                     PKPEKMLAEFGVKKDRKIKKELPVRIRNVNTGEVKEFESIDGAACFLRLKYQAVYQAI
                     KKKTKTRSGWKVEYIKEE"
     gene            23884..24735
                     /locus_tag="KPK24_000035"
     CDS             23884..24735
                     /locus_tag="KPK24_000035"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG2297759.1"
                     /translation="MNVLSLFDGISCGQVAFERARIKVENYFASEIKEIAIKVAMNNY
                     PKTIQLGDVRELNVSKIPKVDILIGGSPCQNLSKGRLIANKIQDGLTGDKSNLFWEYI
                     RILKQLNPEYFLLENVVMPKKDENMISQLLGVEPIKINSNLVSYQNRDRLYWTNILNV
                     QQPKDLKISFQDYKDTSFDYCKEFEIKKTPSREKMWGNGLNGNCKNVTYEDKINCVTL
                     KQDRFSNSGLVEFGSFCRYLTTRELELAQTLPVGYTKGLSIRQAQNVIGDGWTIDVIA
                     HILSNIN"
     gene            25052..25249
                     /locus_tag="KPK24_000036"
     CDS             25052..25249
                     /locus_tag="KPK24_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297760.1"
                     /translation="MNKFQKAVSQMVKQEEKENLLQGYENCIVGRSISSSIRRYVKGF
                     EKFGYSVHEVYEFINDINKYE"
     gene            25276..25692
                     /locus_tag="KPK24_000037"
     CDS             25276..25692
                     /locus_tag="KPK24_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBG2297761.1"
                     /translation="MSKYNNKKIVIDGIKFDSKDESEYYLYLKEKKENGEIKDFGLQQ
                     KFELQPKFKKDGKSYRAITYTIDFAIYKWNGEVVYIDVKGYSTQQGELRKKLFDYKYQ
                     DKKLIWIAKSKKYGVDGWIEYSELKKKRKENKKKVA"
     gene            25711..26211
                     /locus_tag="KPK24_000038"
     CDS             25711..26211
                     /locus_tag="KPK24_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297762.1"
                     /translation="MASKVKKEFFMATKKHLENYKQLHINIESLKLQIKNLKEFHLGD
                     FMQGLSYDSIPISKTNSISNQVENELINLEEKIIEKQIELYEMEALKYTIDVSISNLK
                     PIHKQIIRYRYIEGLEWSLIVDKVYLEERQLRERANQAISSISIALFGKKALIEQEPL
                     FKMLDL"
     gene            26633..26839
                     /locus_tag="KPK24_000039"
     CDS             26633..26839
                     /locus_tag="KPK24_000039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297763.1"
                     /translation="MNRFEGIYSFAEATKLWDLKDSTLRKAVATGKLVENIDCKKFGR
                     DWVVTIEAMEREYGKLNNIMKDLD"
     gene            27328..28515
                     /locus_tag="KPK24_000040"
     CDS             27328..28515
                     /locus_tag="KPK24_000040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297764.1"
                     /translation="MLNEKNLKNIKNILQMVQMKHLKDFENWGINKRKKETAINSLIN
                     IIQNDGEKLDYFKEWISIFTIDGHNNYYVFNYNDSDLELEDLKNIVEDIVSENLLEID
                     TSDLEETKVIYKKNDVINKRLIIRFISPALISCKNENENGNIESGLEKTFYFATLFLD
                     LSLKQIIVSIPHTVGIKSIDNIEAKARDFSEIAKYYVSKLEDLLTTYNIQIEKYNDWI
                     YDATYELAEEGSAHNNPEINKKYEENIDKIDEFAKKIAIASGITDKAIIENFKEGVGI
                     LYENILIDEFGVVEDENQYSTFVQNGDGVNSYCRVGSKTATLKSGKGHAIAKTSRNNE
                     DVKNLGVMKSFNGTLSRFLIEVLDKELYLIRTDTSKFIEERVIYDVIRKVGEYKYTFR
                     KSE"
     gene            28460..28846
                     /locus_tag="KPK24_000041"
     CDS             28460..28846
                     /locus_tag="KPK24_000041"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297765.1"
                     /translation="MMLYEKLENISILLGNQSKAKSIIEELEKLMKSDIITPRLLARK
                     TKVTLLEATRVLEFLVKENELEFFIVVECSNPDSVYENEIGHYKYFTSIKEFNAFSKG
                     QECPICGCGYKYNFEDTKIGFRRLEK"
     gene            28851..29621
                     /locus_tag="KPK24_000042"
     CDS             28851..29621
                     /locus_tag="KPK24_000042"
                     /inference="COORDINATES: protein motif:HMM:NF016362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBG2297766.1"
                     /translation="MSLDTLKEYLSICDDNLIWEPFYDDSMLLDEFSNYIKEIELSKE
                     WNKSKNHEKGKILEQLVRFIMSRFTILSDISVNKSTTDNELDVFVKFNDNIPIPFLCN
                     IKSKIVCECKNWKSRKVDVGMVSKLAEICNKNNAGLGIFISLNGLTGKGWQYAEGKRR
                     KLYLSDKMPIISFTVDEITKLKYKRYNLLTMIKCKMQALIDEMEFAGGELRLLESELG
                     FIDCLNENIESLKMLGLITEVEYNNIKVRINHRYDIVN"
     gene            29679..30362
                     /locus_tag="KPK24_000043"
     CDS             29679..30362
                     /locus_tag="KPK24_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2297767.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWANE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKDKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKYESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDRNDS"
     gene            30340..31593
                     /locus_tag="KPK24_000044"
     CDS             30340..31593
                     /locus_tag="KPK24_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG2297768.1"
                     /translation="MVIEMIVRIDFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTESEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYAHEMDTSEIIKIANSIGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            31599..33038
                     /locus_tag="KPK24_000045"
     CDS             31599..33038
                     /locus_tag="KPK24_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2297769.1"
                     /translation="MLNIYISETDLIKVQLKKESTINLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDITYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEIQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            33028..34521
                     /locus_tag="KPK24_000046"
     CDS             33028..34521
                     /locus_tag="KPK24_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG2297770.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWAIDGLNFSERIWGKYRPTLVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLEVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKYVKNNSKQTGAKYTKGDIEWNIRREEEAELYYDNIRNRKDDISKISKNTNWSE
                     KSIGQIKNHIFYNTHIMRDGTRRMLDSDYSMSVAWQRLINGTYEDIDILLLKHEYLES
                     IFEKKYNISNLEAHRMTEKKHDWYKELIKQKGEFEEDDCLNELIRKE"
     gene            34487..34729
                     /locus_tag="KPK24_000047"
     CDS             34487..34729
                     /locus_tag="KPK24_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297771.1"
                     /translation="MIVLMNLLEKNNEYVIYSYGYEENKLDGRIKIYLDDFYNYEIIK
                     ESKDEHISKSATLKAISKLIKAAKNNDLKKEMSYQC"
     gene            34800..34994
                     /locus_tag="KPK24_000048"
     CDS             34800..34994
                     /locus_tag="KPK24_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297772.1"
                     /translation="MYILTQVIAVVCVVQIFINCIANVNVGILCNKLKEKNEANIDKV
                     SDEILKRVGEELNESLDKSL"
     gene            35039..35602
                     /locus_tag="KPK24_000049"
     CDS             35039..35602
                     /locus_tag="KPK24_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG2297773.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            35614..36504
                     /locus_tag="KPK24_000050"
     CDS             35614..36504
                     /locus_tag="KPK24_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBG2297774.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKFFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            36523..36756
                     /locus_tag="KPK24_000051"
     CDS             36523..36756
                     /locus_tag="KPK24_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297775.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            36766..37167
                     /locus_tag="KPK24_000052"
     CDS             36766..37167
                     /locus_tag="KPK24_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297776.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            37161..37508
                     /locus_tag="KPK24_000053"
     CDS             37161..37508
                     /locus_tag="KPK24_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297777.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            37508..37798
                     /locus_tag="KPK24_000054"
     CDS             37508..37798
                     /locus_tag="KPK24_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG2297778.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            37908..38714
                     /locus_tag="KPK24_000055"
     CDS             37908..38714
                     /locus_tag="KPK24_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297779.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNNLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            39063..39500
                     /locus_tag="KPK24_000056"
     CDS             39063..39500
                     /locus_tag="KPK24_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297780.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPKSNDKSFEIDEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKNNDINKMDTVELFGGLKRGDNFE"
     gene            39493..39669
                     /locus_tag="KPK24_000057"
     CDS             39493..39669
                     /locus_tag="KPK24_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297781.1"
                     /translation="MSKTLSKGTDYKFTKEQIVNSKKYINRKDLLNAILKENELYSFS
                     EVEEIINSFMKGVS"
     gene            39671..40981
                     /locus_tag="KPK24_000058"
     CDS             39671..40981
                     /locus_tag="KPK24_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBG2297782.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDIGLVESSLIYWAAGVIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVN"
     gene            40998..41468
                     /locus_tag="KPK24_000059"
     CDS             40998..41468
                     /locus_tag="KPK24_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG2297783.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            41540..41980
                     /locus_tag="KPK24_000060"
     CDS             41540..41980
                     /locus_tag="KPK24_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2297784.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            42321..42482
                     /locus_tag="KPK24_000061"
     CDS             42321..42482
                     /locus_tag="KPK24_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297785.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     repeat_region   43220..43643
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=43220..43248
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            43807..43980
                     /locus_tag="KPK24_000062"
     CDS             43807..43980
                     /locus_tag="KPK24_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBG2297786.1"
                     /translation="MSSKMGRPKVDNPKNIDVKVRFDENTHKKLLDYCEKENLTRTEA
                     IRKGVDLLLEEDK"
     gene            44104..44931
                     /locus_tag="KPK24_000063"
     CDS             44104..44931
                     /locus_tag="KPK24_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HBG2297787.1"
                     /translation="MNNELMNFENNELGIKIRTIKYEDGSIGINAEDTAVGFGWCQTQ
                     NKNGKQYVSIRWETINKYCKEFGFLNLLGKDEYIPESLFYLLGMKAKNEIAVKFQTWL
                     AVDVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSK
                     LLTAKTLYKKAGIDLPIEIEEEESFFDTKQIASKLKIYSKSNKPAQLAVCEIIKKIDL
                     EENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPAKIAGEKKNYHVVYKIE"
     gene            44994..45170
                     /locus_tag="KPK24_000064"
     CDS             44994..45170
                     /locus_tag="KPK24_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297788.1"
                     /translation="MCENLLNEYNLKTDEDVEYFVKFATLLYKLKQDNEEKFQEYAEI
                     LRGILREQQERENK"
     gene            45358..45570
                     /locus_tag="KPK24_000065"
     CDS             45358..45570
                     /locus_tag="KPK24_000065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297789.1"
                     /translation="MLNSFLDFILTKWYVNVNSFNLLIEDAFRFILTKWYVNVSTFDM
                     CCGVMFMFYINYVDSKLNRKKKHLLK"
     gene            complement(45583..45786)
                     /locus_tag="KPK24_000066"
     CDS             complement(45583..45786)
                     /locus_tag="KPK24_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2297790.1"
                     /translation="MYFNIDKLLESKGRTRYWLAKEVGIAYPNMMKLANNETSSIKLD
                     LFEKLCLVLECTPNELVTLSSKE"
     gene            46030..46416
                     /locus_tag="KPK24_000067"
                     /pseudo
     CDS             46030..46416
                     /locus_tag="KPK24_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726857.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            46480..46833
                     /locus_tag="KPK24_000068"
     CDS             46480..46833
                     /locus_tag="KPK24_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297791.1"
                     /translation="MKLLVTKTFFERAGIELPLEVEEKEHFYDTKQIAKKLGLYTMSN
                     KPAFMAVKQIIRKLDIKEDEMKTVLETNGNWTGTVTKYSNKVTEKVKVWLKENNNPIS
                     IKGDKKNYYILYKNN"
     repeat_region   47265..48216
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=47265..47293
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
     gene            48346..48861
                     /locus_tag="KPK24_000069"
     CDS             48346..48861
                     /locus_tag="KPK24_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2297792.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREDE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     gene            48926..51319
                     /locus_tag="KPK24_000070"
     CDS             48926..51319
                     /locus_tag="KPK24_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG2297793.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIDSITKVADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMAITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVSNVTLGAILIINGLGWVTNATLNVANVFAQNWSIIAPIIYG
                     VIAAIAIYKGVLLASTIATTVASFVNSLYAVAAYKSCAALAAQEFAIFGKISAQTMEA
                     LVTAQATAAQWGFNAALLSCPIFWIIAGIIAFVVVVFVAVAAVNKFSGTSLTVLGAIV
                     GAVFAAVAFIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DAVNNAGNALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYI
                     KQQIDSLNKWVGDAPEKIKLDRMGYKDIGAEFQKGNALGTKWQNAITDKFKDTFDINK
                     MLEDAKKKLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKY
                     LRDIAEQETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMIVSAEGIV"
     gene            51335..52024
                     /locus_tag="KPK24_000071"
     CDS             51335..52024
                     /locus_tag="KPK24_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG2297794.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKIVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGAFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDAENGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKGKKVPKY
                     TIYKGQVLRLV"
     gene            52017..53909
                     /locus_tag="KPK24_000072"
     CDS             52017..53909
                     /locus_tag="KPK24_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2297795.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPAVIDGITWEIERRGSPSK
                     LEFTIAMDDILEFCEGNSVRIYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLNLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDVLLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            53923..54183
                     /locus_tag="KPK24_000073"
     CDS             53923..54183
                     /locus_tag="KPK24_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBG2297796.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            54188..54607
                     /locus_tag="KPK24_000074"
     CDS             54188..54607
                     /locus_tag="KPK24_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG2297797.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFENIKGKVQCKFSVHTKYGNIKAEKVVSV"
     gene            54608..55657
                     /locus_tag="KPK24_000075"
     CDS             54608..55657
                     /locus_tag="KPK24_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG2297798.1"
                     /translation="MFELMTFENIIKRMLDSVPNTLDKREGSIIYNALAPVAVELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIAIERISEKTYKMKCETAGPIFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHKVTVTGVVSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            55650..56267
                     /locus_tag="KPK24_000076"
     CDS             55650..56267
                     /locus_tag="KPK24_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG2297799.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSS
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            56279..57271
                     /locus_tag="KPK24_000077"
     CDS             56279..57271
                     /locus_tag="KPK24_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2297800.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETAKTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESG
                     FKATVTHSLLTQRIVVNIIDATTKENIVPNFKIVDDNSIEIRSEVKVELNVYVINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKVNGGLSNIATSVNELITYC"
     gene            57288..59000
                     /locus_tag="KPK24_000078"
     CDS             57288..59000
                     /locus_tag="KPK24_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297801.1"
                     /translation="MQTEWNFNYANYVQNVSLPPGRYKLECWGACGGAVDTSDWTDCA
                     KGGYSKGEIVFKKRTNLQICVGQSGYEKVSEGSSLTRSGFNGAGAAGKVTTGSFAYSK
                     YGGGATDIRLYHPSATWGNTESLLSRILVAGGGGGMKNNFASARSIGHGGGYVGVNGV
                     GRDRDFCGGGSQYQGGTSYDTEEYHGSLGKGGYGNIGIGGGGGWYGGAGSYSNECGGG
                     GSGYALNKDSYKVPGYIPTPEYYLENIVMTTGGNTTKADGYAKITLLQALPFLTVSSY
                     NSTQATFKADHTDPALLTKIEWFIDDVLKETITTNLTEEKTINYTLEDNALHTLKIVV
                     TDSNNATAEKVLSISKNIMPLPENVNLNDISTKLVEVNAGFKVGKTSIINTLALKNIE
                     ASLNNTLVELSEKIKTSFDSSDTSVQDLQNQVTQKNNTITQLETELSKRKRFITGTYT
                     FTKTDAENFNLSIYDKEGTSKTLTIPVNMGFSPSLIVLSGVTFSTTSKSYVYFDNVCN
                     SNFYNFGYNSDSTHSNPKAVGILNVSNVGYSSLVLTLYKLSMSEAVGIWAKEGATLTY
                     KIYI"
     gene            59021..59368
                     /locus_tag="KPK24_000079"
     CDS             59021..59368
                     /locus_tag="KPK24_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297802.1"
                     /translation="MNRNNRIIYDQTGNIWLQTGEATGDIQEWSKITELNFLDVEFGS
                     IDYSKQYIESINPVTKEPIIKDIEVILTDEQKRLQALEKELSMLKEENKNRDSEIVNT
                     AFEVENIKLNNNL"
     gene            59380..59550
                     /locus_tag="KPK24_000080"
     CDS             59380..59550
                     /locus_tag="KPK24_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297803.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKIDVFYAVNRITEEQYLELTSL
                     LNKEETPAEPIK"
     gene            59581..60147
                     /locus_tag="KPK24_000081"
     CDS             59581..60147
                     /locus_tag="KPK24_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297804.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            60178..60468
                     /locus_tag="KPK24_000082"
     CDS             60178..60468
                     /locus_tag="KPK24_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297805.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            60472..60729
                     /locus_tag="KPK24_000083"
     CDS             60472..60729
                     /locus_tag="KPK24_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG2297806.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGVNQTYKQFKDGEK"
     gene            60990..61853
                     /locus_tag="KPK24_000084"
                     /pseudo
     CDS             60990..61853
                     /locus_tag="KPK24_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
     gene            61932..62744
                     /locus_tag="KPK24_000085"
     CDS             61932..62744
                     /locus_tag="KPK24_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2297807.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            62838..65237
                     /locus_tag="KPK24_000086"
     CDS             62838..65237
                     /locus_tag="KPK24_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG2297808.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NINLKNNNLINELHCDANKLSSLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFL
                     NNNLKSIDLSHNKKLKSLNCTNNKLKSLNLSNNVFLKNLSCSGNNLSKLNIKGLTSLE
                     YLGCAKNKLQDLDVSTNIKLDSLFCGKNSLTKIDISKNKELKSLECSGQYIEKSIIKN
                     GENIVVDIGIENSKLKELYPQNYNISTGFLTWKNISEFPNNVSYSLEYEYGPVNDVET
                     MDVNISIDKLVKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFNGWRNNKD
                     EKIYTSEQVSKLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKLTGADR
                     NETSVKISQKGWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKE
                     INRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNL
                     SKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGG
                     ESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLV
                     DALSVGVLAGKTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLV
                     K"
     gene            complement(65278..65463)
                     /locus_tag="KPK24_000087"
     CDS             complement(65278..65463)
                     /locus_tag="KPK24_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG2297809.1"
                     /translation="MILLEQRKLRVAFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(66011..66187)
                     /locus_tag="KPK24_000088"
     CDS             complement(66011..66187)
                     /locus_tag="KPK24_000088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297810.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            66904..67581
                     /locus_tag="KPK24_000089"
     CDS             66904..67581
                     /locus_tag="KPK24_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2297811.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            67755..69791
                     /locus_tag="KPK24_000090"
     CDS             67755..69791
                     /locus_tag="KPK24_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG2297812.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(69982..70548)
                     /locus_tag="KPK24_000091"
     CDS             complement(69982..70548)
                     /locus_tag="KPK24_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2297813.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            70836..71771
                     /locus_tag="KPK24_000092"
     CDS             70836..71771
                     /locus_tag="KPK24_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2297814.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            72236..73450
                     /locus_tag="KPK24_000093"
     CDS             72236..73450
                     /locus_tag="KPK24_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2297815.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETMNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(73870..74391)
                     /locus_tag="KPK24_000094"
     CDS             complement(73870..74391)
                     /locus_tag="KPK24_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2297816.1"
                     /translation="MKFKRLRTSENKLCYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            74747..75544
                     /locus_tag="KPK24_000095"
     CDS             74747..75544
                     /locus_tag="KPK24_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG2297817.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGIKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            75654..76244
                     /locus_tag="KPK24_000096"
     CDS             75654..76244
                     /locus_tag="KPK24_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG2297818.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            76310..78007
                     /locus_tag="KPK24_000097"
     CDS             76310..78007
                     /locus_tag="KPK24_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG2297819.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            78221..79060
                     /locus_tag="KPK24_000098"
     CDS             78221..79060
                     /locus_tag="KPK24_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG2297820.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT        32098 a         8350 c        13885 g        24815 t
ORIGIN      
        1 acctgattaa tatagtgatt tataaatgag attagaaaat tttatttgat aacaaaaatt
       61 agataatatg aatatataat gtatgtatta aaaatgtggc aaatagttgt tttgctattt
      121 attatttgtt taatatttct aatttataaa aaaaaaatta taaattttaa aaattataaa
      181 tgacatttta actagaatta attgtaaaaa acactatata atactcaatt ttcaacattc
      241 tatacaatta tacagaaatt atacagcata ataatcaccg aaataacaaa ttttaaaaat
      301 taagcaaaac tttttgtcga agttcaacgt ctatatatat gtagtgtaaa tttttgggat
      361 aaaatactac aaaaatataa atgtaaaaat atagtaatat tggaggtcga acgatgagaa
      421 ataaaaagaa aaaaataaat agaaagaagt tgtatttgct tttaagtgct attgtagtgt
      481 gtctggcttt tatagtagtt ggtgttcgcg tcagctcttt aaatatgaag gaaaatgaaa
      541 ttagtagaaa tgcaaaactg tcaagttcta ctattacaga tatagtatct aatacatcaa
      601 tagaagttag taaagggcca agtaaaagtt ctggaaaaat agcatacata actatagatg
      661 atggtccatc taaatttact gacgagatga taaaaacttt aaataaatac aatgttaaag
      721 caactttttt tatgatagat gggaatatga aggaatatcc acagcaagtt aaaaatataa
      781 ttaaaaatgg gaacacagca ggttttcata gtgtatcaca tgatattcac aagctttatg
      841 ttactagtac atcagcaaaa gaggaatttg atacaaatga tcaaactttt tataagataa
      901 ctggtaaaca ttcaaaagta ataagaatac cttatggaag caaaccatat acaccacaag
      961 cctcttatca ggctttagtt gatgctggat ataaaatttg ggactgggac ttagatacag
     1021 aagactggag atctaattct tctcaaattg tacaaaatgt aaaaaatcat attaagaata
     1081 gaaaaggtga agataaagac caacttgtag ttttaatgca tgagaaaaaa cagagtgcag
     1141 aagctttaga ttcagtttta aaattcttat cagatgaagg ttatgaattt gcgccagtag
     1201 atcaaaacca aataccaaaa aattattggt tacgtaattt agagtagtta aaatataaga
     1261 atattgattg atatttttag aaattgagaa taaaaatgag tgaggtgtag gtatattgaa
     1321 taaaaaaatt tatataggaa tggtcggaat attgtctttg atgatggttg gttgtaataa
     1381 aagtttagca aaagtaaatg atgtggaaat cacaaaagaa cagtacaaaa aaacaaaagc
     1441 agttttatct gctactaata attatataaa tgggcagtct ttagatgaat tagaaaaaac
     1501 tttggataaa aaaggtagaa ataaattaga aaatgttata atatcgttta tggtggataa
     1561 tgaactttta tatcaagagg ctaaggataa aggactaact ccaagtaaaa gtgaagtaga
     1621 ctcaaaatat caagaactag aagataagat gaatttgaat acaagctata aggaaaaaat
     1681 ggacaaagca ggtgttgata aagattattt aaaacaagaa atatctagag atttagcaat
     1741 agataaaaat aaaaaggcct ttgaagatag aataaacata agtgataatg atatggaagc
     1801 ctactataca agtcataaaa aagattttaa tgtagaagaa gtaagtgctt ctcagatact
     1861 tatatcgact ttagataaaa ataaaaaaga agtaagcaaa gataaaaaag aagttttgaa
     1921 gaagaaggca gataatatat tgactaaaat caaaaatgga gaaagttttg aaagtttggc
     1981 taaaaaatat tcggatgata aagcaactgg aaaaaatgga ggacagctag gatattttac
     2041 aaaggatgat aaaaatgctg agtttacaaa ggaagtgttc aaattaaaga aaaatgaagt
     2101 ttcaaatgtg tttgaaacta gttatggata tcatatagtc aaagttactg ataaaagaga
     2161 aagacaaaaa agttttaatg agtgtcagag tttaataaga gaatctattt taaatgaaaa
     2221 gtatattgag catataaaaa aactgaatga agatgctaaa atagacagat agaatttcta
     2281 acagctcaat cctagcctgt aatcggaggg agattttaca ggaatgttta agagtttaaa
     2341 gagaacgaaa gttgagaaat acattatcga taataaagac tctttttata gaatagcata
     2401 tagttatacg aagaatgaag aagatgcctt agatgtggtt caagaatcca tgtataaggc
     2461 actatactca gtggaaaata ttaaagaagt aaattatata aagacatggt tttataaaat
     2521 tttagttaga acgagtatag attttattag aaaaaaccgt aaatataata atatgacaga
     2581 tattgacctt atagatgaga ctggagagta tgataaatat actgatttgg atttaagaag
     2641 agctttggaa gaattaccta ttgaatacaa atctattata atattaagat tttttgaaga
     2701 tttaaagatt gaagaagtag caataatatt ggatgaaaat gtgaatactg ttaaaactag
     2761 gttatatact gctttaaaaa aactaaaatt aaaaattgaa gagtagggat aaaaagaatg
     2821 acaagcaaag aaaggttaaa taagttaaaa gatgagtatc ataaaattcc aatacccaag
     2881 aaattagata ctataataaa tcatgaaaaa atagaaaaca tatatcgaga gaaaaataaa
     2941 aaagtaaata gattaagatt taaagttgca atagcctttg catgtatatt tacagtatta
     3001 gtaaatataa gtcctgtatt tgcagacaat ttttctaaga tacctgtaat tagagcaata
     3061 gtagaagtaa taactataaa aaattatagt ttaaaaagtg aaaattatga agctgagatt
     3121 gatattccta aaatacgagg tttaaaagat aaaaacttag aacaaagatt aaacagtagt
     3181 tttatggaag atggaaaaag actttatcat caattccaag agagaatgga gaagatacaa
     3241 tcatcaaaga ataaaggata taaatcttta agtttgagtt atagtgtaaa aaataattct
     3301 aaaaaatttc tatcaataga gatgacaaaa aatgagatag aggcttcatc atatgtgagt
     3361 aaggtacatt atactattga taaaaaaaga caaatagttt tgactttgcc aatgcttttt
     3421 aaagatgata agtatataaa agttataagt gataatataa aagaacaaat gagagaacaa
     3481 atgaaaaagg atagtacaaa atcctatttt atagatcaaa aaaaagactt gcctgttgaa
     3541 gattttaaaa caattaataa gtatcaggat ttttatttta ataaaaatga agaccttgta
     3601 atttgttttg atgaatatga agtagcacct ggttatatgg gcgcagttga gtttgtaata
     3661 ccatataaag taataaaaga tttgtaaatt gaatagtagt ttatatataa atcttttaag
     3721 gaggacaata tgactacata taaaaaactt aaaaaatcta tatttaaaat tgctatattt
     3781 tcaatatgct tattagtttc attagtagat acaaacccta tatttgcagg tagctccttt
     3841 aatgaaactt caaatagtga tacgataaag gtgttaaatg ctaagagtat caactttaaa
     3901 caagacaatt atgaagtgaa tgtgagtatt actaaaataa aaggtttaaa agataaaact
     3961 ttagagaata aattaaacaa tgagcttttg caagatggga aaaaaattta cagtgaattt
     4021 caaaataaaa tgaaaaagga aaataataag ggtcatagat acctaaatgt aatccataat
     4081 gtcaaaaata attctaagga ctttttatca atagaaatga caaaagaaga gatagaagct
     4141 tcctcatcta taactaagaa gcattacact gttgataaaa atagaggaat actattaaat
     4201 ttaccaatat tttttaaaga tagtatatat attaaaacta taagtgataa tataaaagag
     4261 cagatgagaa atcaaattaa gaaagatgat acaaaatctt attttataga ccaagataaa
     4321 gactccccag tgaaagattt tgaatctata aataagtatc aagattttta ttttaataaa
     4381 aatggaaatc ttgtgattag ttttgatgaa tatgaagtag cacctggtta tatgggagca
     4441 gtagaatttg tgataccaaa taaagtaata aaaagactaa gatagatata tagaagattt
     4501 ttagatagac tggtaaggag aattatatga atatatatga acaagcaata agattaatag
     4561 aaaataatga ggatttcgca tttgctacaa taacgtctca ttcaggttca acaccacgtg
     4621 aaactggagc aatgatgatt gtgaaaaatg attctactat atttggctca gtaggaggag
     4681 gtagtgtaga agcagaatgt ataaaacatg caattaatgt tataaaaaac agagaatcaa
     4741 tgttgtataa atttactcta aacaaatctg atgtggcaaa actaggaatg atatgtggag
     4801 gtactggaga aatacaaata gattttatag atagtaaatt aaaaagtaat atagaaaaat
     4861 ttaataagag actaaaggaa aatacaagta aagcatatat atttggtgca ggtcatattt
     4921 caagagatgt tgctgtgata ctatctcttt tagaatttag aacagtagta atagatgata
     4981 gagaagaatt tgctaatcat gaaaggtttc cagaatcaga agtagtagtt ttagattctt
     5041 ttgaaaacat accagatttt ccaactgacg aaaatagtta tataattatt ttaacaagag
     5101 gtcatctata tgattcaagt gctttagaat gggctcttaa aagagaggct ggttatatag
     5161 gtatgattgg aagtagaaca aaaattggat tgacttacga aaaactcatg aaaaaaggtt
     5221 ttaagaagga agaattatca aaagtacatg caccaatagg cataaaattg aatgctcaaa
     5281 caccagctga aatagctgta tgtatagctg ctgaacttat aaattgtaga gctaataagg
     5341 aaaaataaac taatgaacac agggagtaaa gatattgata ctttaaaggt taattttaga
     5401 gggattgaaa taaaagcagt tttagtatat aaaaatagaa aaaatatttc tataaaaata
     5461 gatccatttg gcaaaataac tgtaatgagt cctcctaaaa tatccaaaaa aataataaaa
     5521 gatataatta ttgaaaaagg agactggata cttaaaaagt cagaaaaata taaaggaaga
     5581 gaggaagtat ataaacaaag aatgtttata actggcgaaa aatttcttta tttaggtgaa
     5641 gagtaccatc tagttataaa agaactgttg agagattctg tcaaaaaaag tgactgtaga
     5701 ataactatta acgaatatca aatagttgtt cagacaaatg atacaagtac agattttatt
     5761 aaaaaatctt taaaatcttg gtataagatg gaaagtgaaa gaatagtatt agagagaata
     5821 gattttctta aattaaattg tgaaataatg aagcaattag ttccagcgag tgttaaggta
     5881 aaagaacaaa ataagagatg gggaagttgt acagctcaaa aaaatattta tataaactca
     5941 aaaatatcta tggcaagatt agatgtaatt gattatgtaa taatacatga gtttagccat
     6001 ttagtacata tgaatcactc taaaaaattc tatgatttag taaaatccat tatgcccgat
     6061 tataaagata aagaaaattg gctaaaaata aatggatata aaataataat ctgaaaacga
     6121 ttattccaaa atataggcgt aaagtataaa agaagtatta ttatgaaaaa aataaataat
     6181 ctgaaaattt taaaaaaagt cattgacatt gtaaatgata gggaatataa tctatattaa
     6241 caacaaaaca gaattaaaat ataaaattaa aaaattaaaa gcaatgacag agacaacaat
     6301 actaatagtg ttatccacag agaaccaatg ttgctgagaa ttggtgttaa cagtagtata
     6361 atgatcactc tggagctttt gtactgaata tttttagtaa gtacaaacgt atgtcaacgt
     6421 tacatgacta aggttaaggt aattaattac ccgacctgaa aaaagagggg tagatgtatt
     6481 ttttatatct acaaataagg tggtaccgcg gaatataact ttcgtcctta taagtaatat
     6541 ttatattgct tataaagatg aaagtttttt tattttaagc gatataaata aatttaatcc
     6601 atataaaatt aataaaaaaa taataaaaat aaggagggat ttttatggaa aatactgtat
     6661 atgctaaatt aaaccaagga atagattctt ttttaagagt ggctatgact ctaagaagaa
     6721 gagaggttga tatacaatca ataagtatga ctgtagataa tatgaataat tcaggtataa
     6781 aattaatagt aaatgaagaa aagacatctt tagattctgt cttaaaccat atgaaaaaac
     6841 tacatgatat aagagagata actgttgaaa gaatgagaca ttaattaaat atattttata
     6901 aaactaaaga taaatatgct tattttaaaa taaaaaacta aagataaata ttaaataaga
     6961 aaaatcttag attaaaatat aagtgtcatt gaatttatta gtttatcaca gttaataaat
     7021 ttattagttt gttgtagcta ataaaaactg aaaattctaa aattataatt gtaagattat
     7081 ataaatttag aatttaaatc tgttgaataa tattaaaaca atgataatga gtatgaattt
     7141 ctaataaaaa taaatatcat aaaaataaga aaaagtattg ggaggaaatg aaaatggcaa
     7201 gaatgtatta tgaaaaagat gtggatttag aggttttaaa aaataaaaag gttgcagttt
     7261 taggttatgg aagtcaagga catgcacatg cacaaaatct tagagataac ggtgtacatg
     7321 taatgatagg tctttatgac ggaagtaaat cagcacaaaa agcaaaagaa gatggatttg
     7381 aagttaagag tgtagcagaa gctacaaaag aaagtgattt gacaatgatg ttaatgcctg
     7441 atgaaaaaca aaagaaagtt tatgaagaga gtgtaaagga taacttaaaa gaaggtcaga
     7501 cattagcttt tgctcatgga ttcaatatac attataatca agtacaacca ccagaatttg
     7561 ttgatgttgt aatggtagct cctaaaggac ctggtcactt agttagaaat gtatttacaa
     7621 agggaagtgg agttcctgca ctatttgcag tatatcaaga ccacactaaa aaagctacag
     7681 agacagtttt agcatatgca aaagggatag gagcaacaag agcaggtgtt ttagaaacta
     7741 cttttaaaga agaaacagaa acagatttat ttggagaaca atcagtactt tgtggtggaa
     7801 taagtgaact tataaaattg ggatataaaa cgcttgtaga tgcaggttat caaaaagaag
     7861 tagcatattt tgaatgttta catgaaatga aactaatagt cgaccttata tatgaaggtg
     7921 gatttgaaag aatgagatat agtataagtg acacagcaga atatggagat tatgtatctg
     7981 gtaaaagagt aataactgat gctgcaaaac aaggtatgca aaatgtttta gaggatatac
     8041 aaaatggaaa atttgcaaaa gcgtggatta aagaaaatga agaaggacgt gaaaacttcc
     8101 taaaaactag agaagaagaa tataatacag aaattgctga agttggtaga aatctaagaa
     8161 gtatgatgtc attcttaaaa taaatatata ttaaatggat tagtttgagg gaaagagtgg
     8221 tgtatagtag tgcgtatgaa tggagcaaag gtaattttag aatgtttaaa gaaagaggga
     8281 attgacacaa tatttggata tcctggtgga gccgtgatac ctctttatga tgctctatac
     8341 gattattcag atgattttaa acatataagg acatcccatg aacaaggttt agttcatgct
     8401 gcagatgggt acgcaagaag tactaataca gtaggagttt gttttacaac atctggtcca
     8461 ggagcaacaa atgccataac tggaatagca acagctttta tggattcttc acctatggtt
     8521 gtaatttcag ggcaggtgcc aactagttta ttaggaaaag attcttttca agaaattgac
     8581 ataactggag caacattatc tatgacaaaa cataattatt tagttagaaa cactaaagaa
     8641 ttagttccta caataaaaga agcttttaga gttgcaaatt caggtagaaa aggacctgta
     8701 cttgtagatg ttccaaaaga tttattttta gcagaaatgg attttagtgg tgaggactat
     8761 gatttatgtc aaatagatga ttatatggat tataagagtg attttgatct agatgatgaa
     8821 actaatatta agcttttaaa tgaagcaata gatatcataa aagaatctaa aaagcctgta
     8881 atatatgcag gtggaggtgt taaatcttca gacagtgagg aaattcttga aaaatttgca
     8941 actaagatag atacacccgt actaaataca cttatggggc ttggaaatat agatagaaag
     9001 aatgaactat ctcttggaat ggttggaatg catggaagta gagaaagtaa tttagcactt
     9061 tctaattcag atttagtaat agcgataggt gcgagattta gtgatagagt gataagtaaa
     9121 agttctgaat ttgctaaaaa tgcaaaaata atacacatag acatagaccc atctgaaata
     9181 agtaaaaata tagaatctaa tgtatctttg gttggagatg taaagctagt ccttagttta
     9241 ttaattgaaa gggttgaaag taagaataat agcaattgga aagaagagat aaagagattt
     9301 agaaagagtg agggtgtaca aacatatgaa tttcatccac aaaatatact taaaaaaata
     9361 aatgaaaaat atgaaacatt aaagaaaccc actgtggtag taactgatgt cggtcaacat
     9421 caaatgtggg ctgctaagta ctggaatttt aaaggtaata aaagttttat aacatcagca
     9481 gggttaggaa ctatgggatt tggtttaggt gcagctattg gaactaaagt tggtaatgta
     9541 gataaaaatg tggttttagt cactggtgat ggaagtttta gaatgaactg taatgaacta
     9601 gctacagttg caaattataa tgtacctatg cttattttac tactcaataa tagaacatta
     9661 ggaatggtaa ggcaatggca aaagttattt tccaatcaaa gatactctca aactgacatt
     9721 aatgaaaatg tggattatgt taaactcgta aatgcatata atattgatgg atataaggta
     9781 tccagtatag aagaattagg aaaggcgcta gatatgatag attttaataa acctgtattt
     9841 ttgcaatgtg atatagataa ggattatgat gtttatccta ttgtagcacc taatgatgca
     9901 ttggaaaatt tgatatgtaa ctaaaactta tactgaatat tgataaaatt ttttattaaa
     9961 tttttattgg tattcagttt ttgttgttta gagatagatt ggcatttttt attttttcaa
    10021 ataaatcaat gactatataa ttaagaactt attaaatttt aattatacaa tttggaatag
    10081 tattacaact taagtaaata ttaagtttta ttcgttagat ttattattaa taccttatat
    10141 tagttatggt tgttatatac tctaaagcct tgaaattaca agtgtatagc tgactatata
    10201 aatttgataa ttttagcttt taaaatagat aaattagatt ttgccaccca tttgccaccg
    10261 tattatattt ttgggtggca aactctaatt tgtaattttt tcaaatatat ctacagtttc
    10321 atttttcatt ttatcagtta catgtgaata ggtatccatt gtagttgata gttggctatg
    10381 acccaaacgg ttttgtatgt ctttaatgtt agcaccattt tctaataata gagtagcatg
    10441 tgcatgtctt aaagaatgaa aatggaagtc attgtttaaa gctactcgaa tttgtcttac
    10501 tatagtgtct aaagtgtgag tattcacttg ttgaccattt tctttggtac atacccaatc
    10561 actatcaaag taaaattctc catattttaa tttcattttc ttttgatata atttatgttc
    10621 ttttaatgcc tttattaaag tgtcacctgt aaatatagtt ctgcaagaac tttctgtttt
    10681 tggttgccct aattcaaaca ttccatttgg ttttttaatc aaagtatgct ttactgtgat
    10741 agttttatta tcaaggtcta tattatccca ttttagtgca ataatttcac ctcttcgcat
    10801 accagtatga aatccaatta gtaaaactat acgttgaaat gaatcttgag gaaatatatt
    10861 tagtatttga ttaaattctt ctaatgtaat agttttaact ttattagttt ctgttttaga
    10921 tttagttttt ggtatgctta catattgcat agggttttct cgtatgtgtt tgtaaggatg
    10981 gactgctgat tttaatgacc tatgtaatat ggcttttaat acttgtaatg tattttgaga
    11041 gtaatcatct ttgtactttt tatttatgaa gttttgtagt attgcaggag ttaaagcttt
    11101 tactttgtaa gctcctagtt ttggctttat atgtttttct atgtttattc ggtagctttc
    11161 ttgagtgttg tatttacagt taagtaagac atattctttg taccaaaagt ctaagtagtc
    11221 tgataaactg atattgcttt cttcaaatac tatgccagag ttttcatatt catttagtgc
    11281 ttctcttaag gctttttctg cttctttttt agtattgcct ccaactcttt ctactttttt
    11341 tctttttcct tctactatgc ctaggtcaaa gtagtaatac catttgttac ttctttttct
    11401 tactccacct ttcataatag tatccctccc ttttagaatg tatgtttgtt tggtgtttat
    11461 ataaaagagc agattaactg ctctttatat acttttgtta tgtacaatat tatcttaatt
    11521 ttgtttatct ggttttcact taatcatttt cttaatattc ttacaatttt ttagctttta
    11581 tgtgtagttt attataataa tattgaaata tatatttatt aagtaaaaaa tctataaaag
    11641 tagaataaat gagtttaaaa aatatcctca tatatgtaga aatttggcaa attagttaat
    11701 actaaaaatc tattatttcc taagtccaac atgcttttct tttttgatag aaattctaat
    11761 ctttttagta agaagttgat gctaacttgt aattcttcag ctatttcgta tacacttgta
    11821 gcgtgtgaat taataacatg tattatttct tcttctgtta taagaaattc acatgcccat
    11881 tttagtgctt tgttttcagt tttgtctatt aagattttat ttttgtaact gttttttgaa
    11941 gatacatagt ttccaacact ggtaaaatga tgtcctaatt cttctgctaa gatttctatt
    12001 agtttagcat tgttttgttt taatgaatta agtaatgata taatctttag tccttgtctg
    12061 tttatataca atccttttat gtcatctgct attttgtcag tgtagtaaat ttctatctct
    12121 tcattatttg ctaagtctaa aagtgcgtct agtttgttca ttgaaatccc cctataaaaa
    12181 gaatgtattt ttgatttttg ttatatataa agagcaggtg tgaactgctc taaatattca
    12241 ttttttatcc aaaaaagatt ggataatttt agctgtagac aaaatatgga ttatagtata
    12301 tgattataat ataattacat ataatatatg taagcactta caaatgtgtg ttagtatagt
    12361 tagcactaaa tttgtatttg tgcttaaagc gattaaagga gttttggagc ggtgggtaga
    12421 gttttactca ctataaccaa gactcttttg catattagag tatttcatgt agtcatatat
    12481 tctaataagc cttcttaaat ttgttttttc gcttgttttt gcagactcta aaatattagg
    12541 attttcctca aatattttct taagtagatt ttctaactgt tcagtgtcaa tttctttttc
    12601 ttctaaaaaa gcaataagtc ttgtagcttt ttcaaatgat tgatgttcat cattccaaat
    12661 accataaaca cttctatttt ttatgtaagt attatttcta ttttttttaa tactattgct
    12721 aataatattt tcaaagtcac tttctagtgc aattgtccta taatctggaa attcaagtgt
    12781 gctattaaaa agatgtttat ttaatggtaa tttattatat tgcttaatta aagatggata
    12841 agcatattca agtaattcat ctgatgaaaa ttctaagtca ttcatgacta tatcacgata
    12901 ccattcatca aaacttaatc cttttaatcc tttaagacca aaatcacttg ccttgccaac
    12961 tgctaaaact aaatcttcat cttttattct tgtatcatca acactaccaa cccgaatttt
    13021 agcagtaggt ttgttagtaa ttgtgaaatc gtaaaattct tgtttgaaca ttcttaatat
    13081 tctaactggt aattttgatt ttttttcaga taatgcctca tagagtaaat taaaatcctc
    13141 cagtttaatt ttagtcatag taatcatttt attatcaaaa acaattgtat gacttgatat
    13201 ttctatatca ttaaagtttt tttcatactc aatgaatatg aaacgttcct cgagtttttt
    13261 agcattttca ttagatagac aattaactat agcattaagt atttttttta tatttacatc
    13321 tgtaacagag taaccaataa atatgatagg aaactcaaca aatatagtca ttagcttagc
    13381 tgccaagtag gcacttttgc tgtcaaagtc tatataatca gcttcattta ttacaatact
    13441 atttggagaa gaaacacatc catgaatttt ataaatttct gcaatacctt gtatagatga
    13501 aaaaactaaa ttttcttgac ctatatatgt tgtataatta tcaacgatag tttccaaaaa
    13561 acaatcataa tttgttgtaa taaatcctga taaacttttt ttagagatat tactaagttt
    13621 ttcaacttca tcttgatatt tatctactat tttagaatta tttttaatat acatagcaat
    13681 ttctgcctta aatggagaaa ctcctgattt aacaatatct aaatacttag agtctaagct
    13741 tcgtatagaa gaatctgaaa accatttttt attaaaatca ttttcaataa gttctgcaat
    13801 tctaggattt atacccattt ttgggttctc tgattttgct ttatttttat aacttgtata
    13861 tataaagtca tcattactaa ttttttctgc aaagactttt agtaaatcct cccaatttgg
    13921 aagattatag tatcttctag ttaaaccaga accaacaaat aaaaatggtg tagcattaaa
    13981 tcggtttatg acatctgata ttatagattt catataattt atcacctact tttaatttat
    14041 tttttatact tatttattaa aaattcaata taatcattga gttgttcttg agcttcctct
    14101 ggtaaatctt tgtgaggatt aactctatgt gcagctacag tgtctatatc attttttaca
    14161 atagacctac ctaataaata atcagtagtc acattaaata tttcagcaag gctagatagc
    14221 atataagcat caggaactgt ctttccttgt tcataataac cataagcgct tttggttaag
    14281 tttaatttat ttgcaacatc ttcttgagtc atattttttt cagctctaag ttcttttagt
    14341 cttttttgaa ataatgtatt attacaagaa gttttgctag aaatattgct tgctttatta
    14401 tcatacctac ctattaaata atcaacagta gtgccaaaat attcagctaa actagataac
    14461 atttctgggg taggtgttct tttaccttgt tcataaaatc catatgcact agtagttatg
    14521 ttaagatagt tggcaatttc tttttgcatt acaccttttt ctttcctaag aaatttcaat
    14581 ctatctgata aaatgctcat ataatcactc ctcttaataa agttgccttt attaaataat
    14641 acaacaaata gttgtaaaat gaaataaaaa aataaaaaaa ctatttacaa acaacatata
    14701 gttgtgtatt atataagtat aaacaacaaa atgttggtaa ggaggatata gcttgaacaa
    14761 tctaaaggaa cttaggacag aaaggaattt aaagcaaaaa gatgttgcat taaaagtagg
    14821 tataactact agttattatg gaatgattga aagtggaaca agagtaccaa gtttagcaat
    14881 agcaataaaa ttatctaaat gttttggagt atcaatagaa aaaatttttt taaatacaaa
    14941 tacaacaaga tgttgtttaa aagagaggag ttaaatatga aagatttaaa aatagtaaaa
    15001 gttaataata aactaacaac tgacagtaga gatatagctc taatggttga aaaagaacac
    15061 aagattttac taagggatat aagaaattat ataaaccaaa tggaagaagc caataaaaac
    15121 atgagtacag atttgtaccc atctgattat tttattgaaa atacttattt agatgattat
    15181 aaaagagaga agccatgcta tgcaataaca aagataggtt gtgacttcat agcaaataaa
    15241 atgacaggca taaaaggtac ggcatttaca ggaatatata caaaaaaatt tgaagaaatg
    15301 gagcaagtat taaaaaatga acaacctaaa ctaccaacta catataaaga agcattacaa
    15361 catcttatag agcaagtaga agtaaatgag aaattacaac tagaaggaaa aatgaaagac
    15421 caagtaataa aagaactaaa accaaaggca gattatacag atatgatact taaaaacaaa
    15481 ggacttgtca caataactca aatagcaaaa gactatggaa tgagtggaaa agaaatgaat
    15541 aaaatacttc atgaaagagg gattcaatat aaacaaagtg gacaatggct tttatataaa
    15601 caacaccaag ggaagggata cactcattca gaaacaatag acataactag aagtgatgga
    15661 atgactgatg taaaaatgac aactaagtgg actcaaaagg gaagattgtt tttatatgac
    15721 ttattaaaag taaataacat attaccagat atagaaaaag agtatagtta tcaaacttca
    15781 atgttatgtt agtactttga aaaataaata cagaatattc aaaaagaggt gatttaaagg
    15841 ttgagtaata gaaagaagta taccttgagt attacgaaag aattatacaa taaatgtaaa
    15901 gaaaaagcta atcaaagagg tatgtcagtg aatgattaca tactatcagt gattagcaag
    15961 agtatataaa ttattcttca atattaattt taatttctcc ttttctttgc tcataatctt
    16021 caatatgttt ttttattatt tgttcaattt ccttattagc agaacgacct tcgctttcag
    16081 ctatatattt aattttttca agcagaactc tgtttattct taaagtgtat ttaggtagtg
    16141 aagttgacat aataatacct cctttttaaa gtctaaatga tgtcataatt atatcataaa
    16201 aataatttat agaaaatcta taaaatatat tgacggcata aagacggcgt gatattatta
    16261 aaacaagaaa ggggttgacg gcaaaatgaa ctcaaataaa aagagaataa ctgttaggat
    16321 gccagaaaaa ttaaatgaag aaataacgaa aaaatcaaaa tacttagggt taactaagaa
    16381 ctcatttata ttagatatac tatggaagga atttgagtta ttagaatata gaagttataa
    16441 aaaggaggta gataaacatg aataacttac aagtaataga aagaaataat gagagagttc
    16501 taactacaca acaattagca gatgtatatg aaactgactc aagaaatata agtaacaatt
    16561 tcaacaataa caaagataag tttattgaag gtaaacatta ttttttattg caaggtgatg
    16621 atttaaagaa ttttaaaggc attcatacag aatatgaaaa cctaaaattt gcttcaaaaa
    16681 tgtatctttg gactgaaaga ggagcaaata gacactgcaa aatattagat acagataagg
    16741 cttgggaaca gtttgataat ttagaggaaa catatttcaa agttaagcaa cataagccaa
    16801 cttgtataga agatgtatta atagaaagtt taaaagaaat gaaagattta agacttcaag
    16861 ttaatcaagc aaatagcatt gctttagaag caaagacaga ggttgaaaca ataaaagatg
    16921 tagtttcatt agactcaaat agttggagaa caaatacaca tcaactaatt gcaagaatag
    16981 caaaaaaaca aggtggtttt gaacatataa atatgcttag aacagaaagt tatgaattat
    17041 taaataagag atttggagtt gacctacata gaagattgat caataaaaga agaaaaatgg
    17101 cagaagaagg tgtatctgaa tctaaaagag agaaagttaa caatttagat gtaatacaag
    17161 atgataagaa actcatagaa gggtatgttg ctattgtaaa agatatggct ttaaaatatg
    17221 gaatatcaag tgatttaagc aaaaattaag ttaaatcaca acagcacttt gaaaactaaa
    17281 tataaaataa aaatatctcc cttaaaagtt ttttaaagag agataaacac tttattttgt
    17341 aactatttga atacatgatg gatgaatgca atagttttta tctttgtaat ttatagaaac
    17401 ataattacta gagtacattt tatcaaggtt ttcattaata tattcaattg cattacagcc
    17461 atttatagag ccaatatcag ttatatcaat ccagttatta aatatcttaa cataaacaaa
    17521 cttcatgata acaccaactt tcattagaat ttaagttaat tataacatgg tgtactgaga
    17581 taatgtgtta agaaatagat acagaatatt caaaagaggt gattagatgg aaatagagca
    17641 aacaacaata cgcctgctaa aaagcagacg aaagttgaat attaatttct attttggtta
    17701 ggcgaaagac ctaacaaaaa atctgtagat acattaagtt tgttagaaat taatattaaa
    17761 gtttctagat ttggttctct tgaaccagct tcataatact gatatgcacg ttctgagata
    17821 ccaaacaaac tagcaaattg cttttgagtc atattcattt gctttcttac tagcttaata
    17881 ttatctttga atttagtcat aactacacgc tccaataaaa aaataaataa atttcttgac
    17941 acgaacaata ttagcgtgta atatataaaa catagcacga acaatattgg cgtgttaaat
    18001 aaaaaggaga gggtgaaaat aaataataat ttaaaactac aacgtgaaaa aattggctta
    18061 acacaattag aggttgctca aaaagccaaa ataacagaga gaagctatca atattatgaa
    18121 gctggcgaac gtctcccaaa cattcgtaca gctttaaaga tagctataat tttaaatact
    18181 aattgtgaaa aactctttaa tgaataatag cataaaagga ctcagattac aagaaaggag
    18241 ctttagatat gaataatttg caacttaaca ataaaaatac aataacaaca ttagaaattg
    18301 ctgatatgtt agaaatcaga cactgggaag tcttaaggaa attagaagga acagaaaaaa
    18361 caaaaggaat tattgatatt cttaacgaca acaattttgt tgtggttgat tactttataa
    18421 aatcaacata tttggattca aaaaatgaaa gtagaccttg ctataatgta acaaaattag
    18481 gttgtgactt tctagcaaat aaatttactg gagaaaaagg tattattttt acagctagat
    18541 atgtaagaag atttaatgaa atggaacgag tattaaaaga acaacaacct aaactaccaa
    18601 ctacatataa agaagcattg caacagttat tagtagaagt tgaagaaaaa gaacaattac
    18661 aattagaaaa tcaagaaaaa gataaggtaa ttcagttaca gcaaccaaaa gtactgtttg
    18721 ctgatgcagt ttcagcttca aacaattcta tattagttgg agaacttgca aaacttataa
    18781 aacaaaatgg agtagatata ggacaaaaca gattatttac ctggatgcga gaaaatggat
    18841 atttaattaa gcgtaaaggt gaagattata atattccaac acagaaaagt atgaatctaa
    18901 gaataatgga agttaaaaaa agagtaatta ataatcctga tggaagtact aaagtaacta
    18961 gaacagtaaa aataactgga aaaggtcaag tttattttgt taataagttt aaaagtagta
    19021 aacaattatc aatgttaagt taaatcataa cagtaccttg aaaactaaat acagaatatt
    19081 ttggaaagga gagattgaat tggaaaataa gaaaatagaa gaaatagaga aaagaataac
    19141 catgcttgaa aatcaattgc aagcacagtt atttagtcag ataacagctc aatcaaatag
    19201 tgattttata gattatatgt accaatgtat agttttgtat ttacatgaga tgaataaaag
    19261 tgaaaaaaga aaaattagtg aaattactaa agaaagaatc agaaaataag aatgataatt
    19321 acaatataaa acctattctt ctagcaggtt ttgaagggtc agttttcttt actgaagtta
    19381 tcataaagtt tatctgtgat gtgtgttgaa gaatttcaga ataaactcca tttactatgc
    19441 cgtagtagtg aattagacaa gggttatgat aacctacttc ttcaacaatc attaatacat
    19501 cttttccaaa gttagtcaat tttagtgcaa tttcatgttc atcatcaagt tctttttcaa
    19561 attcctctat agatttaaga ataattttat attgaaattc agcatagtaa aaatactctg
    19621 tgttagaagt atcagagact gtctttctta tactttcttg taattcttta gatatattgt
    19681 ttgaaaactg cataatacac catccttttg aaaattttgg aatttattcc atgtttatat
    19741 tataccatgt agaactgagg tgaatacaat gttaataggc gacaatataa agcaaatact
    19801 aagaaaaaga gatataaaac cttataaatt agcaaaggaa ttaaatatag atgtaagtgg
    19861 tctatataaa ttgttgaaga ataaaaattc taatccaact atagatactc taataaaatt
    19921 agctgattat ttagatatta cattagacga attagttgga agataaatat ttgaaaagag
    19981 gggagagaat tttaacatgg atatttctga aagtataaca aagcaattta gtgatagttt
    20041 aaaaagttta atagaaatag agataaataa acaagaaaca gatagagtta agagtcaaac
    20101 tgttgaacaa aaggtaaaag tactggagcc taaagatata gttgttttaa taaaaagagg
    20161 ttatccaaat tatttgataa cagtagaaga agcaagggga attttaaaat tagatacagt
    20221 ttttatgcgt aggttagtga gcacaggttt gataaaatca ctggctagag gtgatggtag
    20281 aaaaatttca agatatgaag ttgatgattt tattgaaaga aatcaaggta aaaatttgga
    20341 tgaactttta aaagtagcag agagggggga tgaaattgtt aagccttaat actaataaga
    20401 ataatatagt aactcttaaa aaagatggaa aagctatagc agacatagta tttaaagata
    20461 ttaaaactgg taagaaaata tcggttggag tattaaataa aaaagtgctg gtcaaataac
    20521 caacacacaa aaaaataaaa aataaaatat aacacaaaca aattatagca caaaaagaat
    20581 ggatttagaa taggtaatat aacattctaa aaggtttaat gtgtaataat tgtaacaatt
    20641 tagaatgtag cttatataga tagtaataaa ttgggaggga tttaaatgga agcagctaga
    20701 ctaatagcaa taggtcaaat taaacaagca gaaaaagaaa tatgtaaatt acaaggtaca
    20761 aaaaataata gtagtttaat gtggtgggaa gccgtaaaat ttgctagtca aaatatatta
    20821 gagggtcttg aacatgacat tgagttagaa gcttctattg agtttagaga agctatgatg
    20881 tatcaagaag aacttgaaaa agatagacca atagatgtcc agatataaaa aaagagcctt
    20941 cgcacaggct ctaagtgaaa ataagttaca aaaattatag gtatattata acataagggg
    21001 gaataaatga aaacaaaaaa tgaaataatt aagggtttag aagatagatt atttttatta
    21061 agatttacaa cagtagatga agtagattgg gatgtaaaat ttggacaaat atcagcatta
    21121 gaattttgta tagataaaca tagaaaagga tgcactttgg aacaattcaa agaacattta
    21181 gacgaataca aattacaagg gaactatggt gattatatag atggttttgt gtcagtttta
    21241 gaaagaaata ttagagaaat ggagggagaa attgatggaa gtgaataata tttacataaa
    21301 attgatggat gtaagaatta agtttaacaa attagatata aaaaagagtg gtcaaaacaa
    21361 gtttgctaac tttaaatatt ttgagttagc agacttctta cctcaagcaa cagagctgtt
    21421 acaagaagct aaattatgcc ctatagtgac ctttacaaat gattatgcaa ctctaacatt
    21481 gattaatgga gagaaaccaa cagaagagat aatatttact tctccaatga gagaattaca
    21541 attaaaaggc tctaatgagt tacaagcatt aggaggaatt gaaacatatc agacaaggta
    21601 tttgtacatt cagttattaa acattacaga aaatgatact tttgatgcta ctagtggaaa
    21661 agaagattat aaaagaaatg acttaacaaa ctcctctatt aaagcaagtg aaaatggtca
    21721 aataaaaata agtcaaaatc aaataaaaag attgttttca ataggaaata caataggcaa
    21781 agattcagat agagtaaaaa gtgaagtgta ttataagttt aataaagaag ttaaagattt
    21841 aagtaaacag gaatatgacc agatatgtgt tggatatgag aaattacaaa gggaaaaagg
    21901 aataattaag taggtgaatt tcttgaagga cagagacaaa gcaactatag agaaaggaaa
    21961 tatacttagt gatgggtatg gtctttcacc acaattggta gctagagatt catggctaac
    22021 aacaggagca agagctttat atttctatct atccagtttt gcaggagcaa gtgggacatg
    22081 ttatccatct agggatatta tgactcatga acttggtata aataaagata cttttagtaa
    22141 atacctaaat gaactaaaga cgagtggtta tataaaagta tataaaaata aaactaggga
    22201 aggaaggatg caaaataata tatatgaggt agtatttgat agaagctata tagaaagtca
    22261 tatatccatt agatgtaaga aagaaaataa aaaaaaacca tgtccgaaaa aagcagacat
    22321 ggaaccatgt ccgaatttac cggacatggt tcaaccggac atggaaaagt cggacactat
    22381 aagtaacagt attataaata acagttttaa aagtagtatg tatatagagc aagctgtgga
    22441 taactcttta aaagaattta agaagctata tgaagaaaat ataggagtag tatatccagt
    22501 cacagctgaa tggttattag aagtatctaa tgaagtagat ataagagtat ttaaaagagc
    22561 tatagagata tgtgctgaaa gaatgaatat gaatttatca tacttaaaag gaatccttaa
    22621 aaagtggaag gatgcaaata taactacata tgaacagtta gaatcatata aattacaaca
    22681 tgaaaataaa aagtcaaaaa aaaccaatag tgtagtaagc aaaaataagt ttgctaattt
    22741 tgaacaaaca tttactcaat acagtaacaa agaattagat gaaattataa agaaaagcca
    22801 aaaggctaaa tttaaataat attgatggag gtattaaaat gaatcaagtt gtattagttg
    22861 gaagattaac tagagaccca gaactcaaat acataccagg aacaggtaca gcagtagcat
    22921 catttacaat agctgtagac agaaattata taaataaaga aggaaaaagg gatactgatt
    22981 ttataccaat agaagtaata ggtaaatcag ctgaatactg tgcaaattac ataacaaaag
    23041 ggaagctagt agcattagaa gggaatataa gagttgacaa ttatcaaact caatcaggtg
    23101 aaaaaagaat atttacaaaa gtcagtacaa aatcagtaca atcattagaa agcaagaata
    23161 aatcgagtaa ttcatataaa gagagtgtac aagatggaac cataggacta gaccctcaag
    23221 gatttgaaat tatagatgat gatgagttac cattttaatc tgaaaaatta aatatgaggt
    23281 gaaataaatg tttaaagtag aaaggtattt tagtggctca gtagtggaca accttattga
    23341 agatgacctt acatgtagaa actacttagc attatattgt tgtttgttgg gaattacaaa
    23401 aaatggaaaa aagatatatc ctaagccaga aaaaatgtta gctgagtttg gagtaaagaa
    23461 ggacagaaaa ataaaaaaag agttaccagt aagaattaga aatgttaata caggggaagt
    23521 aaaagaattt gagtctatag atggtgcagc ctgtttttta agattaaaat atcaagcagt
    23581 ttatcaagct attaaaaaga aaactaaaac aagaagtggc tggaaagttg aatacattaa
    23641 ggaggaataa tggaagtttc aaggacagaa tatacaatta aaagagcaaa agagttatat
    23701 gacaatggag aggatatatt tattgctata gataaggcta gagaaaaata tgaggagatg
    23761 attaaaagtg aatatcttag ctagtgtgat attagtaata ggaagtttta tagctggtag
    23821 agtttatgag tatagattga acctaaaagg gtgtgaaaat tgtgataata agaaggtgca
    23881 aatatgaatg tattaagtct attcgatgga attagttgtg gtcaagttgc ttttgagaga
    23941 gctagaataa aagtagaaaa ttattttgca agtgaaataa aagagattgc gattaaagta
    24001 gcaatgaata attacccaaa aacaatacaa cttggagatg taagagagct aaatgtaagt
    24061 aaaatcccta aagtagatat tcttattggc ggaagcccat gccaaaattt aagtaaagga
    24121 agattaatag ctaataaaat acaggatgga ttaactggag ataaaagtaa tttgttttgg
    24181 gaatatataa gaattctaaa acaattgaat ccagaatatt ttcttttaga aaacgtagtg
    24241 atgcccaaaa aagatgaaaa catgataagc caattgttag gagttgaacc aatcaagata
    24301 aatagcaatt tagttagtta tcaaaataga gacagattat attggacaaa tattttaaat
    24361 gttcaacaac ctaaagattt aaaaattagt tttcaagatt ataaagatac tagttttgat
    24421 tattgcaaag agtttgaaat taaaaaaact ccaagtagag aaaaaatgtg gggtaatggt
    24481 ttaaatggaa attgtaagaa tgtaacatat gaagacaaaa taaactgcgt aacattaaaa
    24541 caagatagat ttagtaattc aggtttagta gaatttgggt ctttctgcag atacttaaca
    24601 actagagaat tagagttagc gcagacgcta ccagttggat atacaaaagg tttgagtata
    24661 agacaagcac aaaatgtcat aggtgatggt tggacaatag atgtgatagc acatatttta
    24721 tcaaatatta actagttctt tatatataaa ttattattga agcggttagc tatcatgata
    24781 cttgtagcag taagttttat agctggtagg gtttatgagt atagattgaa tctgaaagag
    24841 tgtgaaaatt gtaataacaa ctatcctgaa aaatgagaaa gaagtggttt tatgaataaa
    24901 agaataattt gcaattggtg tggtaaatta ttttacatcc caaaacagtc taaaaaaatt
    24961 tactgttgta aaagatgtga gagaaaggct aagaaaagca atagagaaca gcaaaattaa
    25021 ttttaaacaa taaaactatg ggggaatagc aatgaataag tttcaaaaag cagtttctca
    25081 aatggtgaag caagaggaaa aagaaaactt gttgcaagga tatgagaatt gtatagtagg
    25141 tagaagtata tcaagttcca taagaagata tgtaaaaggg tttgaaaagt ttggatatag
    25201 tgtacatgag gtttatgaat ttataaacga tattaataag tatgagtaac tttaagtgat
    25261 aaaggaagta tttgattgag taaatacaat aataagaaaa ttgtaataga tggaattaaa
    25321 tttgatagta aagatgagtc agagtattat ttatatttaa aagaaaaaaa ggaaaatgga
    25381 gaaataaaag actttggact tcaacaaaag tttgaactac aacctaaatt taaaaaagat
    25441 gggaaaagct atagagctat tacatataca attgattttg ccatatacaa atggaatggt
    25501 gaagtcgttt atatagatgt gaaagggtat agtacacagc agggtgaact tagaaaaaag
    25561 ctttttgact ataaatatca ggacaaaaaa ttgatatgga ttgctaaaag caaaaaatat
    25621 ggggtagatg gttggataga atatagtgaa cttaagaaaa agagaaaaga aaataagaaa
    25681 aaggtagctt aaataaatag gagtgatgtt atggcaagta aagttaaaaa ggagtttttt
    25741 atggcaacta aaaaacacct tgagaactac aaacaactac atattaatat tgaaagtcta
    25801 aaacttcaaa taaaaaatct caaagagttc catttaggtg attttatgca aggtttaagc
    25861 tatgacagca ttcccataag taagactaat tcaataagta atcaagttga aaatgagtta
    25921 attaatcttg aagaaaagat aatagaaaag cagatagaat tatatgaaat ggaagcacta
    25981 aaatatacaa tagatgtatc cataagtaat ttaaaaccta tacataaaca aattataagg
    26041 tataggtata ttgaaggctt agaatggagt ttaatagttg ataaagtata cttagaagaa
    26101 agacaattaa gagaaagagc taatcaagcc attagttcaa tatcaatagc cttatttggt
    26161 aaaaaagcat taatagagca agaaccatta tttaagatgt tagatttata ggcagttaat
    26221 aactgtctat tttttttgtg aaaaagatat atggaagtta ttgacaatat cacgcatacg
    26281 tgatattatt gaagtataga aaggaggcga aaagaaagtg gctaagaaaa taaaagagtt
    26341 cagaaaactc ataaaagaat taactgaact cgtactcgaa gttggcacac tattagcagt
    26401 catcaaaatg gtaatagata gcctacatta gtaaaatgtt aagagtggaa gttgcacctt
    26461 ccctcttaac taaattataa aacatagtca ctaaaaatac aatgaataag tatagagagt
    26521 taacatttga attattgaaa ctactgtatg acactattaa attaattgga gcatcaatat
    26581 tgttatatta tgttattaac tcaattttct agcaatgatt aggaggtatt ttatgaatcg
    26641 atttgaaggt atttatagct ttgcagaagc aacaaaatta tgggatttga aagattctac
    26701 acttagaaaa gcagttgcga caggcaaatt agttgaaaat attgattgta aaaagtttgg
    26761 tcgtgattgg gtagtaacta ttgaagcaat ggaaagagaa tatggaaaat taaataatat
    26821 aatgaaagac ctagattaaa ttctaggtct ttttattgcc gcttttctgc cgattttaca
    26881 atttaaaatg tgagataata gtattgtgga aatgaatatt tctctctcaa aactaaatat
    26941 atgtgggcta ggttaaggga ttcgcctagc ttatatgaac aaactaggca gggcatgagg
    27001 atgctgtaag ttcaattctt actatgttca aacttattaa tacactatat gtagatatgc
    27061 tggattaaaa cggaatttaa ttcaaatgtc taaaagagtg gggcttggta acctcactca
    27121 atttgcaagg actggtgtgt aatcttaggt tcgattccta aaacttgctc ccttaaatat
    27181 aatatgtatc cccaaaaaaa ggcttagatt aaattctaag tcttttttta ataattaatg
    27241 tagataataa tatttattgc taaaatgatg tacaaatatg ataatataaa ttacataaag
    27301 atatataaat agagggggat tataaatttg ttaaatgaaa agaatctaaa gaacataaag
    27361 aatatattac aaatggtaca aatgaaacac cttaaagatt ttgaaaattg gggaattaat
    27421 aaacgaaaaa aagaaactgc tataaatagc ttgataaaca taatacaaaa tgatggagaa
    27481 aaactagatt attttaagga atggatatca atctttacaa tagatggtca taataattat
    27541 tatgttttta attacaatga ttctgacttg gaattggaag atttaaaaaa catagtagaa
    27601 gatatagtaa gtgaaaattt attggagatt gatactagtg atttagaaga aactaaggtc
    27661 atatataaaa aaaatgatgt cataaacaaa agattgatta taagatttat ttctccagct
    27721 ttaattagct gtaaaaatga aaatgagaat gggaatatag aatctggatt agaaaaaact
    27781 ttttattttg caactttatt tctagactta agtttgaaac aaataatagt ttctatacca
    27841 catactgtag gtataaaaag catagataat atagaggcta aagcaagaga tttctcagaa
    27901 atagcaaaat attatgtatc aaaattagaa gatttgttaa ctacttataa tatacaaata
    27961 gaaaaataca atgattggat atatgatgct acatatgagc ttgctgagga aggcagtgct
    28021 cataataatc ctgagataaa caaaaaatat gaagaaaata ttgataaaat agatgaattc
    28081 gcaaaaaaga tagctattgc ttctggaata actgataagg ctataataga aaatttcaaa
    28141 gagggagtgg gaatactcta tgagaatatt ctaatagacg aatttggtgt ggtagaggat
    28201 gaaaatcaat acagtacttt tgtgcaaaat ggtgatggag taaattcata ctgtagagtt
    28261 gggtctaaaa cagcaaccct aaaatcagga aagggacatg ctatagccaa gacatctaga
    28321 aacaatgagg atgtcaagaa tttaggtgtc atgaaatcat tcaatggaac tttatccaga
    28381 tttttaattg aagtcttaga taaagaatta tatttaataa gaacagacac ttctaaattt
    28441 atagaagaga gggtgattta tgatgttata cgaaaagttg gagaatataa gtatactttt
    28501 aggaaatcag agtaaagcaa aatctataat agaagaatta gagaaattaa tgaagagtga
    28561 tatcattaca ccaagattgc tggccaggaa aactaaagtt acacttttag aggctacaag
    28621 agttcttgaa tttttagtta aagaaaatga attagaattt tttattgttg ttgaatgttc
    28681 taatccagat agcgtatatg aaaatgaaat aggtcactat aaatacttca cttcgataaa
    28741 agaattcaat gctttttcaa aaggacaaga atgcccaata tgtggatgtg gttataaata
    28801 caactttgaa gatacaaaaa taggctttag aagattggag aagtgataca gtgagtctag
    28861 ataccttaaa agaatatcta agcatatgtg atgataattt aatttgggag cctttttatg
    28921 atgacagtat gctattagac gaattctcta attatataaa agaaatagaa ttatctaagg
    28981 aatggaataa atctaaaaat catgagaaag gaaaaatttt agaacaatta gtaagattta
    29041 taatgtctag atttacaata ttaagtgata tatctgtaaa taagtctaca acagataatg
    29101 agctagatgt ttttgttaag tttaatgaca acataccaat accattctta tgtaatataa
    29161 aatcaaaaat agtatgtgaa tgtaaaaact ggaaaagtag aaaagtggat gtaggaatgg
    29221 tatctaaact agcagaaata tgcaataaaa ataatgcggg attgggcata tttatttctt
    29281 taaatggatt gacaggtaag gggtggcaat atgctgaagg aaaaagaaga aaattatatt
    29341 tatcagataa gatgccaata atatcgttta cagtagatga aataacgaaa ttaaagtata
    29401 aaaggtataa tcttctgact atgataaaat gtaaaatgca agcattaata gatgagatgg
    29461 aatttgctgg aggagaatta agattattag aaagtgaact tggatttata gactgtttaa
    29521 atgaaaatat agaatctttg aaaatgttgg gtttaataac cgaagttgaa tataacaata
    29581 ttaaagtaag aatcaatcat agatatgata ttgtaaactg agaactccaa acgagttctt
    29641 tttttattcc caaaacgaca aacaaacgag gtggtggtgt gcaagatgtg aaagaaaaag
    29701 taaaacaaga ttacctaaaa ggtatgaaac aaaaggaaat atcagcaaag tatgacatta
    29761 gtttaaatac tttaaagtca tggataaaaa gatacaattg ggctaatgag aaaaagaagg
    29821 gtgcacctaa aaataaaaga ggtgcaccca taggtaataa aaatgccact ggtcctcctg
    29881 gaaataagaa tgctgaaaaa tttggtttct tctcaaaata cttacctgaa gaaacccaag
    29941 acttaattaa tgagataaag gataaagata aatttgatat tctttgggaa cagataacaa
    30001 tacaatatgc agcaataata agagcacaaa agataatgta tgttaaagac aaggaagaaa
    30061 tgattaagga attaaagaaa tatgaaagca cagaaaatgg tgagaagata gagtatgaat
    30121 ttcaatttgc atgggatagg caagcatctt ttcttaatgc acagagtaga gcaatgagtg
    30181 aacttagaag tttaattaaa cagtatgatg aaatgattca taaggattgg aatttggcta
    30241 cagaggagca gaaaacaaga gttgagaagt tgaaatgtga agttgataac ctaagtaaag
    30301 atgatattgg agatgatgag ttgaaaataa gtgtagatta tggtgataga aatgatagtt
    30361 agaatagatt ttaatccaga tttcaaggaa gccaatttta ctaaaaaaag atacagagca
    30421 atgaaaggtt cagcagggag tggaaaatct gttaatgtag cacaagacta tatactaaag
    30481 ttaggagata agaagtatca aggagctaat ctattagtag ttagaaagtc agaagctaca
    30541 cataagtatt caacgtatgc agagcttaca ggagctataa atcgtattta tggtaaacaa
    30601 gctgataagt attggaaaac tactttaaat cctttagaaa ttaagagtaa agttactggt
    30661 aactctataa ttttcagagg agttaatgat gcaaaacaaa gagaaaaatt aaaatcaatt
    30721 aacttctcga aaggaaaatt aacatgggtt tggtgtgaag aagctacaga acttatggaa
    30781 agtgacatag acatactaga tgaccgttta agaggtattt taactaatcc taacctatac
    30841 tatcaaatga catttacatt taatccagtc tcagctactc attggataaa aagaaagtat
    30901 tttgactata aaaatgatga tatatttact catcatagta cttatctaca aaatagattc
    30961 atagatgagg cttactacag aagaatgcaa atgagaaaag agcaagaccc agaagggtac
    31021 aaagtctatg gtcttggaga atggggagaa actggtggag caatacttaa aaattatgtt
    31081 atacatgaat ttcctacaga atctgagtat tttgacaata tgaggttatc acaagacttt
    31141 ggatttaacc atgcaaatgt agtacttaga attggcttta aggatggaga gttatatata
    31201 tgtaacgaaa tatatgcaca tgaaatggat acctcagaaa tcataaagat tgcaaatagt
    31261 ataggtttag aaaagactct atttatgtac tgtgatagtg ctgaaccaga tagaattaag
    31321 atgtggaaga gtgcaggata taaagctaaa ggagttaaaa aaggaccagg aagtgttaaa
    31381 gctcaaatag attatttgaa acaattaaga atacatgtac atcctagttg cactaatacc
    31441 ataaaagaaa tacaacaatg gaaatggaaa caagatgaaa gaactggatt atatcttgat
    31501 gaaccagttg agtttatgga tgatgcaatg gctgcgctta gatattctat agataataag
    31561 cttaaaaata atggaataag cttcttaaag taaaggaggt gttaaatatt tatataagtg
    31621 aaacagattt aataaaagtt cagttaaaaa aagagagcac cattaaccta gtaaaagtca
    31681 tagaacacta catcttaaag catagaccag aaaaatataa acaaggagaa gaatactatt
    31741 atggtaatac tgatgtaaac aataagagaa gatattatct cttagatgga gctaaggttg
    31801 atgattttac taaagttaat aataaagcaa ttaacaacta ccataagctt ttagttgacc
    31861 aaaaggtagg ctatagtgtc ggaaatccca tagtatttaa tgcagatgat gataatctca
    31921 ctaagctttt aaatgactta ctaggagaag agtttgacga tacaataaca gaactatatc
    31981 tcaatgctag taataaaggg gttgaatggt tacatccata tattaataga aaaggtgagt
    32041 ttaaatatgt aataattcca gctgaagaag caattcctat ttgggatagt aaaagacaga
    32101 gggaattagt tgcatttatt aggttttatt atattgaaga tatagatgga aataaaataa
    32161 aaagagttga gtactacaca gaaaatgaca taacttactt tattgaaaga ggtaatagtt
    32221 ttattcaaga atttttatat gatgaatatg gaaaaatgac tgatatacaa gaaggtcatt
    32281 ttagaataaa taacaaagaa cagggatggg gtaaagttcc atttatacct tttaaaaata
    32341 atgaaaagtg tgtctcagat ttaactttct ataaatcatt aatagatata tatgacaata
    32401 atatttctac actagcagat aacttagatg aaatacaaga ggttatttat gtattaaaag
    32461 aatatccagg aacaagtcta caagagttta tagataatat aagatactat aaatcaatta
    32521 aagtagatgg tggaggtgga gttgataaac tagagataaa tataccagtt gaagctaaaa
    32581 aggagcttct tgatagattg gaaaagaata taattatctt tggtcaagga gttaatccag
    32641 aatctcaaaa cacaggtgac aaatcgggtg tagcacttaa atttttatat tcactacttg
    32701 acttaaaatg ttctaagact gaaaagaagt ttaaaaaagc aattagagag cttttatggt
    32761 ttgtgtgtga gtatttaaag ataagtggta gtaagagcta tgattataaa acagttcaaa
    32821 ttacttttaa tcactctatg ataataaatg aagctgaaaa gatagatatg gcagctaaat
    32881 caactggaat tgtatcagat gaaactattg tttctaacca tccttgggtc gaggatgtta
    32941 atgacgaact tgagagactt aaaaaacagg aagatactca aaaagagtat gatgatttaa
    33001 ttcctaataa tcaagatggt gttatagatg aaacataaag attattggag aaagagattt
    33061 gaacaattag aagaagctca aaataacaaa agtgtaaaat attatcttga attagaaaag
    33121 caatataaac tagctataaa tagtatagaa aaagatatat tagcatggta caacagattt
    33181 gccaaaaatg aaggaatatc tttattagaa gctaagaaac tactaaatac aagagaacta
    33241 gaagagttta aatggagtgt tgaagaatat attaaatatg gtaaagaaaa tgctataaat
    33301 caaaagtgga tgaaagagtt agaaaatgct agtgcaagag ttcatataac aaggcttgaa
    33361 gctttaaagt tacaaataca gcaacaagta gaagttttat atggaaatga acttgatggt
    33421 attgataaac taatgagaga tatttataca agtggatact atcatacagc ttttaatgtt
    33481 caacaaggag taaacgttgg ttggagttta atgagtcttg atactaatag aataaataaa
    33541 attatctcta aaccatgggc aatagatgga ttaaacttta gtgaaagaat ttggggtaag
    33601 tatagaccta ctttagtaaa tgaactacat actaagctaa ctcaatcaat tattagaggt
    33661 gaaaatccaa aaaatttagt aaatgacttt gctaagagat ttaatgtatc taaatcacaa
    33721 gctaagaatt tgataatgac tgaatcagct ttctttgcat cagcaagtag aaaagattgt
    33781 tttagtgatt tagaagtaga gaaatatgag attattgcta cattagattt aagaacttca
    33841 aatatatgca gagagttaga tggaaaaata tttgatatga aagattatca agttggaata
    33901 acagctccac catttcattg tcgttgtagg acaacaacag ctccttggtt cgaggatgaa
    33961 gaaggctata gagcagcaag aggagaagat ggaaaaacat attatgtacc atctagtatg
    34021 aagtataatg agtggtatga gaagtatgtt aaaaataata gtaaacaaac tggtgcaaaa
    34081 tatactaaag gtgatatcga gtggaatata agaagagaag aagaagcaga actatattac
    34141 gataatatta gaaatagaaa agatgatatt tccaaaatat caaagaatac aaattggtca
    34201 gaaaaaagta taggtcaaat taaaaatcat attttctaca atactcatat aatgagagat
    34261 ggaactagac gtatgttgga ttctgactat agtatgtcag ttgcttggca aagacttata
    34321 aatggtacat acgaagatat tgatattctc ttattaaaac atgaatacct tgaaagtata
    34381 tttgagaaaa agtataatat aagtaactta gaagcccata gaatgactga gaaaaagcat
    34441 gattggtata aagaattaat taaacagaaa ggagagtttg aagaagatga ttgtcttaat
    34501 gaacttatta gaaaagaata atgaatatgt tatatatagt tatggatatg aagaaaataa
    34561 gcttgatgga agaataaaaa tatatttaga tgatttttat aattatgaaa taataaaaga
    34621 gtcaaaagat gaacatataa gtaaatcagc aacgttaaaa gctatttcta aacttataaa
    34681 agctgctaaa aataacgatt tgaaaaaaga aatgagttat caatgttaga agcacttact
    34741 gaacaataaa ttagtaggtg cttttattat gtaaaagttt aaaaaagtag gtgattttaa
    34801 tgtatatatt aactcaagtt atagctgtag tttgtgtagt acaaatcttt attaattgta
    34861 ttgctaatgt caatgtaggt attctttgca ataaattaaa agaaaaaaat gaagctaata
    34921 tagataaagt ttctgatgaa attctaaaga gagtaggaga agaattaaat gaatcactag
    34981 acaaaagtct ttaaagactt tttttattgt gtaaaaaatg aaaggagata tttaaaagat
    35041 ggattggtta aaagaattgc tagaaggaat aaaaatagaa aataacaaaa ttgatgtagt
    35101 ttctcttcaa aaatctatag aaaagaaaat aaaagagact acaattactc aagaagatta
    35161 tacaaatctt gaaacacagc ttaatacagc taatgaaact attaaaaagt ttgaaggagg
    35221 tatgacaaaa gaagatgtag agaatctaaa aacaacttat gaaactgata agaaaacttt
    35281 ggaagaaact tacaaaaaag aaattgaaga aaaagatttt aattactggt taggtgatgc
    35341 ttttaaatct gttaaatgta gagatgaaat agcattaaaa gctcatttag atatagaagc
    35401 actaagaaat agtaaagaca gacaaaaagc ttttgaagag caaataaatc ctttgaaaca
    35461 ggataaagat tatttgttta atgcaacact agaaggtgaa gaacctaaaa tagatactat
    35521 aacaccaggg caagagccta agataaatga ttttggtttt aattttactg gggtaagacc
    35581 tcatgaaaat aataataaat aggaggaaat aaaatggcag cactaaatta tgcaaaagaa
    35641 tattcaaatg ttttagcaca agcatatcct tatactttaa acttcgggga tttgtatgca
    35701 acaccaaata atggaagata tagatggact ggttctaaaa caatagaaat accaactata
    35761 tctacaactg gaagagtaga ttcaaacaga gatacaatag cagtagctca aagaaactat
    35821 gataatgctt gggaacctaa ggtattaact aatcaaagga aatggtctac attggttcat
    35881 ccagcagata taaaccaaac taattatgtg gcttcaatag gcaatataac aaaagtatat
    35941 aatgaggaac aaaagtttcc agagatggat gcttactgta tatctaaaat atatgctgat
    36001 tggaccgcat taggtaacac agcagataca actgttctta caacagcaaa cgtattagaa
    36061 gtatttgata agttaatgga aaaaatgaca gaagctagag tacctgaaaa tggaagaata
    36121 ttgtatgtta ctccagtagt aaatacactt atcaaaaatg caaaagagat acaaagaaca
    36181 gtaaatataa aggatgcagg aacttctctt aatcgtcaaa caactgatat tgacacagtt
    36241 aaaataatta aagtaccatc taatctaatg aaaactgcat atgattttac aactggatgg
    36301 aaagtaggag caggagctaa acaaatcttt atgtccttag ttcacccaag tgcaataatt
    36361 acacctgttt cttatcagtt ctctaagtta gacgaaccaa cagcagttac agagggaaaa
    36421 ttcttctact ttgaagaaag ttttgaggat gtatttatat taaataaaaa agctgatgca
    36481 atacaatttg ttgttgaagc atagaaaaag gagtgatata tagtgccaca agtaaaaaaa
    36541 ctaaatagaa tactaaccat agaagaatgt aaaatagatg atttcttaga gatgggatat
    36601 gatttgatag atgaaactgg taaggcagta aagtatggca agtcattaag tgtaaaagat
    36661 ttaatagctg aaaataatat tttaaggtca aaagttgagt ctttagaaga agaaaataag
    36721 cagcttaaag agaaaaataa acttactaaa aagtaggtga aaattatgga aaataatata
    36781 attgatgaaa tagaaaaaag acttgaaagt tttggatata tattaaaaga tggagataag
    36841 tggttaatag gttttgtaag agaaaaaata gaaaatatta ttaaactaga ttgtaatata
    36901 aaaactatgc caattgaatt gaaagaaatt gaagttgata tgatagttgg agagttctta
    36961 tttaccaaga aaaatatggg tcaattagat atagaaagca ttaactttga agctgtagaa
    37021 aagtctatat cagaaggtga tacaaaggta gattttgcta taggaagtgg ttctcaaaca
    37081 ccagaacaac gctttgatag cttaatagct tatcttacta cttatggcaa gaataagata
    37141 ttaaccttta ggtgcttaag atggtaagta aaactagaaa agcaatagaa atgttatata
    37201 gagataaatg tactatagtt gagtatcagc caattaaaga ccctgtaaca aaacgaacta
    37261 acaataaaga agtgattgta ttagaaaatc aaccatgtaa actttcatat aaaaatatag
    37321 tttctgctac agaagggaaa gtagctaagc tagagcaaac tattaaactc tttatatctc
    37381 cagatataga aattaaagca ggttcaaaac ttattataaa tgataaagag tatgtaagaa
    37441 gtggagaatc agctatatat ccaaatcatc aagaaataat acttgagtta tttaaggata
    37501 aagcataatg gctagatggg gcagtgttga ttttagagag tttaaaagag tttgtaaaaa
    37561 gatggaggag cttacaaaga ttgatttaga taagttttgc aaggatgcag caagagaatt
    37621 agcagcacga ttacttggga aagtaattag aagaacacca gttgatacag gattcttacg
    37681 acaaggatgg aatggagtgg cttatgctag gtcgcttcct gtgtataaac aaggaaataa
    37741 ttatattata gaggttgtta atccgactga atatgcctta acacaatggg gcatataaaa
    37801 cccagcaaaa tcggtaaacg ctaagtgtaa tagtgttaca ttataaagta ggtagaatat
    37861 ggacatagaa taactcttga tttataatgt ataaaagagg tgattttatg gcaaaatttt
    37921 tagatattac aggaaaaaaa tttggaaggc taagagtcat taaattttct aaagagataa
    37981 aaagtggtaa aagaaataga aaatattggt tatgtaagtg tgattgtgga aatttcaaag
    38041 aaataagaac tgactcttta actagtggct tagtacaatc ttgtggatgt ttaaaaaaag
    38101 aacaagataa attaaattta acagacaaat accaatttaa aaagaaatat aaggttcaaa
    38161 ataaaagact ttacagtata tggaagggta taatatctag atgtacagat aaaaataata
    38221 aaagatacaa tagatatggt gaaaggaata taattgtgtg tgatgaatgg ttttgctatg
    38281 ataattttgc aaattgggca ttgagtaatg gatattcaga gaaacttaca attgatagaa
    38341 taaataatga aggaaattat gaatcaagca attgtagatg ggtggatata aagacacaat
    38401 gtagaaatag gtcaacaaat atattagtaa agcatgaaga aaaagaaatt acattgatag
    38461 agctttctga aaaaacaggt atttcatatt cttgcttaag aagtagatat tctaagggat
    38521 tagtaggaaa taatcttata gaaaaagtta agattattga agaaagcaga gctaagttat
    38581 ctattgagga tgtaaaagaa attagaaaaa agtattctga tggatataca ataaaacaac
    38641 taagtgaaat atatcctgta acatattcat ctatatcaaa tatagtccat agaagaacat
    38701 ggaaaaatat ttaattaatt atatatgcca ataccgagga aaacctatag attgcgaata
    38761 ggctataggt ttccgtagag cgtagagagt gaataaatat aatctctcca agagtgctgg
    38821 gcaactaaat aaagtttatt ttgttgatga tgtacgccga acttatagga aactataaga
    38881 gctagaggat aaaaagcctt taggataaca aaatgcatat gttgaatatg gccatagaac
    38941 taaagatgga aaaggttggg ttaaaggaca acatttctta acaatttcag agatggaact
    39001 acaaagccaa gttgataaga ttatagagaa aaaactatta atattgctta aaggagtatt
    39061 tgatgcttaa taatataatt gatggaatat ctattaaatt agataaaaca tttggagaga
    39121 gttatacaat ttatagtgaa gatgtggagc aaggtataaa tgaaccttgt ttttttattg
    39181 ttcctttaaa tccaagcaaa gtatcctatc caagtggcag gacattaaaa aagaactctt
    39241 ttgatgtaca ttattttcca aaaagtaatg ataaatcatt tgaaatagat gaggtagctg
    39301 agatgctact ggaggaatta gagtatatag aaattgatgg agatttagtc agaggtacaa
    39361 atatgaactt tgaaattgta gataatgtac ttcatttctt tgttgattat aactacttta
    39421 ctataaaaaa taatgatatc aataagatgg atacagtaga gttattcggt ggtttgaaga
    39481 gaggtgataa ttttgagtaa gacattaagt aaaggaaccg attacaagtt tactaaggag
    39541 cagatagtta attctaagaa gtatataaat agaaaagact tattaaatgc aattttaaaa
    39601 gaaaatgagt tatattcctt ctcagaggta gaggaaataa taaatagctt tatgaaagga
    39661 gtgagttaat ttggcgttag gtggaggaac atttgtaaca cagaataaaa tattaccagg
    39721 tagctatata aattttatct cagctaagag ggcaaccagt tcattatcgg atagaggtat
    39781 tgttgcaata cctttagagt tagattgggg catagatgaa gacgtatttc aagtaaccag
    39841 tgatgatttt gagaagtatt cagtgaagta ttttggatat gattatactc atgagaagct
    39901 gaaaggtttg agagatttat tcaaaaatat aaggttggga tatttttata aattaaataa
    39961 aggcgttaaa gccagttgta ctatagccac agcaaaatat agtggtatca gaggaaatga
    40021 cttaaaagta acagttacaa caaatataga tgataatgct aagtttgatg ttgtaacact
    40081 tttagataat aagaaggtag atactcaaat agcaaaggtt attacagact tacaagacaa
    40141 tgactatatc acttggaaga aggatgcaac actagaagca agtgcaggac ttgtatttac
    40201 tggtggaact aatggcgaag ctgtgacagg agcagagtac caagctttct tggataaaat
    40261 agaaagctat agctttaatg ctttaggatg tttggctaca acaacagaaa ttaaaagttt
    40321 atttgtagaa tttacaaaga gaatgagaga taaggtagga gctaagtttc aaacagtact
    40381 atataagaaa agtgatgcag attatgaagg tgtagtgtct gtagaaaata agattaaaga
    40441 tattggatta gtagaatcta gtttaattta ttgggcggct ggagttatag caggatgcga
    40501 tataaataaa tctaatacta ataaaaagta tgatggtgag tttgatgttg atgttaatta
    40561 tacacaaata caacttgaag aagctttaaa aactggtaaa tttatattcc acaaggtggg
    40621 agatgaagtt catgtgttag aggatataaa tacttttgta tcatttacag atgataaaaa
    40681 tgacgatttt tcaagtaacc aaagtgttag agtacttgac caaattgcta atgatattgc
    40741 aactttattt aatgaaaagt atttaggtaa agttccgaat gataaggcag gaagaataag
    40801 tttctggaat gatgttgtta aacaccataa agaattagag aatataaggg caatagaaga
    40861 ttttaaaact gatgatgtta gtgtagagct tggaaatgat aagaaaactg tcatagtatc
    40921 tgatgctgtt aaggtaataa atgctatgag taagctttat atgactgttt cagttaatta
    40981 gagaggagag tgataatatg gctcaaacaa taaatgctaa agatacagtt agtgcaaaga
    41041 aagctgaatg ttttataact atagaaggta aaagatataa ttttatgcaa gctatagatt
    41101 tagaggctaa aatggaaaaa aataaaagtg aagttccaat tctaggaaga acaacaaagg
    41161 gaaataaaac aactgggagt acaaatactg gaagtgcaac atttcattat aatacttcta
    41221 tttttagaga attactttac agatataaag aaactggtga ggatatttat tttgacatac
    41281 aagttacaaa tgaagaccct acatctgctg taggaagaca gacagtagta cttaaagatt
    41341 gtaatatgga cagtggaata attactaaat ttgatgctga tggtgagtat ttagatgaag
    41401 atatggattt cacttttgag gattgggaat tagtagaaaa atttaattta ttggcaggaa
    41461 tggagtaaaa tacacattta taaattatat atgtgtattt tttatatgaa aaattaaaat
    41521 aaaaggagat tagaataata tgagtaattt aagtgctttt ttaagtcaaa atgcaataaa
    41581 ggttgataat gtaaaatatg tagcgagtaa cagattttta gataaagaag ggaaaccagt
    41641 tgaatgggaa ttaaaagttt tatcatctga agaagacgaa gcactaagaa gaaagtgtac
    41701 taaaagagta aaagtgattg gtaacaatgg taagcatact ggacaatata caagtgaaat
    41761 tgactacaat agttatgtag ctgaattatg tgtagcatct acagtatttc cagatttaaa
    41821 ggatgccgaa ctccaaaata gttatggagt aatgggagaa gctcagttat taaagacaat
    41881 gcttacagca ggtgagtatg tcaattatac agtaaaagtg aatgaagtca atggatttga
    41941 tacatctttt gaggataaag tagaagaagc aaaaaactaa tcagaggtgg cgattttgat
    42001 gctagcatca ctcattattg tattcaaaaa ttaaagtgga agccaagtga atatatgaat
    42061 ttagaagtta atgagagagc gttagcagcc gcctcaatac ttataaagat agaagatgaa
    42121 gaggaagcaa tgaaagaagc tgaaagagag agaaagaggg gacgaagaag atagcaaaat
    42181 aaaaaaataa atatagaata ggtaaaatat gtaataatta tatgttataa tatttttagc
    42241 aagaagatgt aatctacaat ttatagagtg gagttcatac tgggataaaa cctacttcct
    42301 aatgaaagga ggtgggaagt atgaataact ttttacttaa tgtaatagct ggcgttattg
    42361 ctagtttaat attttgctta atttgtaaag tatttctaaa agtaaaaagc cactcaactc
    42421 gtggcaagag taaaagtggc tgggaatttg attttaaaat caagttccat aagttcaaat
    42481 agattcattt aattatgaac ttcactctac cgcaaaatag attgtagttc ttcttgcttt
    42541 tattatacca caaattagaa aaaatattgt ttatataaaa taaaaaataa aaatttttat
    42601 taaaaaattg aaaacttgat tataaagcaa ttaatttata aaatatatat aaataagtag
    42661 gtatttattt acttgaattt cattgtttat ataaaaaaaa tggaaaaata tgtaataatt
    42721 atatgttata ataattgtag caaggataat aatcgaaagt gcgaagggtg attattttca
    42781 tattaaacgc caaattccaa ataaggaagg aggtgaaatt atatgatagg ttttttatta
    42841 agcatactag ctggtgttat atcagcttat atttatgaca aaataaaaaa tcacccagac
    42901 gccaataagg gtgatttaaa aaaataattc tttaaatcaa ttttgatgga aatagctact
    42961 cttgtataaa gtaaattatt tccttgcttt tattatacca caaattggta cagatattca
    43021 aaaataatat ttttatgata taataaaaat gtagagattt tgcagtgagc aatatttgcg
    43081 ataaattgaa gtttaacaat tggaatacaa ggtattgagg gtgtgtgata aatgttatca
    43141 attgcactac tcatggttca ctgcaaattt gagagagatg cgtatgtgta ggtattggaa
    43201 atgccaagtt tattttgggg ttttagatta actatatgga atgtaaatta acctttttat
    43261 tgttgttgtt attcttattg ttattcgttt tagattaact atatggaatg taaatgttgt
    43321 aagaagtatc attctatttt ttaatctttc tgttttagat taactatatg gaatgtaaat
    43381 agtacatata atgagtcttt aacatcagtt atgaaaggtt ttagattaac tatatggaat
    43441 gtaaatgatt gtactttagc gtctgcacta gctttgtcta tcgttttaga ttaactatat
    43501 ggaatgtaaa ttattttaca gatgaacaat tacagttact tcttgaatgt tttatattaa
    43561 ctaagtggta tgtaaataat ggaagcaaga taatttttaa aggtatggat aattgtttta
    43621 tattaactat gtggattcaa aattaaatag aaaaaagaaa gcacttactt aaatagtagg
    43681 tgcttttgtt ttgctcaaaa ttggtcggtt gagtaaaata attagaaaaa attgggataa
    43741 gttattgact ttttggaaca caaataatat aattatatta tggaacacaa aaagtgaggt
    43801 gaaacaatga gttctaaaat gggaagacca aaagttgata atcctaaaaa tattgatgtc
    43861 aaagtaagat ttgatgaaaa tactcataaa aaattattag attattgtga gaaagaaaat
    43921 cttactagaa cagaagcaat aagaaaagga gtagacttac ttttggagga agataagtaa
    43981 aaaagtagcc caacgccgac caaagcatac tggactactt aacctagagt tatctctata
    44041 tgaaatattc tatcatgtaa agataactct ttcaagataa taaatcgaaa gggtgatttt
    44101 tgtatgaata atgaactgat gaattttgaa aataatgaat taggaataaa aataagaact
    44161 attaaatatg aagatggaag tataggaatt aatgcagagg atacagctgt agggtttgga
    44221 tggtgtcaga cacagaataa aaatggtaag caatatgtat caattagatg ggaaactatt
    44281 aataaatact gtaaagaatt tggtttcctc aacttgttgg ggaaagatga gtatatacct
    44341 gaatcattat tttatctttt aggtatgaaa gctaaaaatg aaatagcagt aaaatttcaa
    44401 acatggttag cagtagatgt actaccatca ataagacaaa ctggtgcata cataactaac
    44461 aatgctaatc ccgaaaaact aagagaaaaa gcaagtgaga ttgaaaagtt acaattagcc
    44521 tataacagca catctatgtt aaaagaattg ctagatggtg caggatttga caataaatcc
    44581 aaactattaa cagctaaaac attatataaa aaggcaggca ttgatttacc tatagaaatt
    44641 gaagaagagg aatctttctt tgacacaaaa caaatagcat ctaaactgaa aatatattct
    44701 aagagtaata aaccagcaca gttggctgtt tgtgaaatta ttaaaaagat tgatttagaa
    44761 gaaaacgaag tcaaaggcgt ttgggaaact aatggttctt ggactggtac tgtaaataaa
    44821 tatacaaaga gtgtaataga taaggttaga aattggatag aggaaaataa tagacctgct
    44881 aagattgcag gtgaaaagaa gaattatcat gtggtttata agattgagta aatttatcag
    44941 ttgtattaaa taatttagtt tagtttattt tagttttgag ggggattaat acaatgtgtg
    45001 agaatttact taatgaatat aatttaaaaa ctgatgaaga tgtagaatac tttgtaaagt
    45061 ttgctacatt attatataaa ttaaaacagg acaatgaaga aaaatttcaa gagtatgcag
    45121 agatattgag aggtattctt agggaacaac aagagagaga aaataagtaa aataatatag
    45181 ataaagcact tgaatattac atagtttcaa gtgctttgtt tggtataaaa tggtataata
    45241 gagataagag ttatattaac tatgtggtat gtaaagaatg tataaggcat cttctcaaat
    45301 tctgcgttgg tcattttata ttaactatgt ggtatgtaaa gtgcgttaac tgcaataatg
    45361 ctgaactcat ttcttgattt tatattaact aagtggtatg taaatgttaa ttccttcaac
    45421 cttctaatag aagatgcttt taggtttata ttaactaagt ggtatgtaaa tgtttcaaca
    45481 tttgacatgt gctgtggagt aatgtttatg ttttatatta actatgtgga ttcaaaatta
    45541 aatagaaaaa agaagcactt acttaaatag taggtgcttt tgttattctt tgctagataa
    45601 agtaacaagt tcattaggtg tacattcaag aacaagacaa agcttttcga ataaatctaa
    45661 ttttatagaa cttgtttcat tgttagctaa tttcatcatg ttaggataag ctatacctac
    45721 ttctttagct aaccaatatc tagttcgacc tttggattca agtaatttat caatgttaaa
    45781 atacattttt tcacctctta agtatattat acaacaatta tataatgttt gcaatatata
    45841 atatgcaaac aatataatta tataatgttg acattatata atgtgtgcga tataatatac
    45901 ttaaggaata aaaaataaga gccactcgcc cctaccaagt ttgagtaact cttattgaca
    45961 tatactacat atacattaat tatagtatat gtcattcctt aaaaaaaatc aattaaggag
    46021 tgtgttttta tgaacaattt agtactaatt aacaatcaag aagtgcaagt taaggaattt
    46081 aataatcaaa gagtagttac atttaaagaa attgacagag tacatgaaag agtagaagga
    46141 acagcaggta gaaattttag agaaaataag aagcacttta taaaaaatga ggactatttt
    46201 tatttagaag gaaaagaatt atcaacaatt aaacagacaa cgaatttcgt tggcagtaat
    46261 gcaagagaat taatcttatt aactgaaaca ggttatctaa tgttagtaaa atcttttaca
    46321 gatgatttag cttggaaagt acaaagacaa ttggtaaata gttattttag agttaaagaa
    46381 gaaaagaaag aaatgaaagc attagaaaaa ttagaaacag ttaatgagtc aattaggtta
    46441 ataactccaa tttttgatga tttaaacatt gataaaagta tgaaacttct tgtaactaag
    46501 actttttttg aaagagcagg tattgaattg cctcttgaag ttgaagaaaa agaacatttt
    46561 tatgatacaa agcagattgc aaaaaaatta ggactttaca caatgtctaa taaaccagca
    46621 tttatggcag ttaagcaaat tataagaaag cttgatatta aagaagatga aatgaaaaca
    46681 gtattagaaa cgaatggcaa ttggactggt actgtaacaa aatattctaa taaggttaca
    46741 gaaaaggtca aagtgtggtt gaaagaaaat aataatccta tctcaatcaa aggagacaag
    46801 aaaaactact atatattgta taaaaataat tagattttag tttttggggg ggtaatacaa
    46861 tgtatgagaa tttacttgat agtatagata ttgaaaagag aaaagaagaa tttagaatta
    46921 aacttttgaa aataagagaa acagatatag atatatatat ataataagat agaaagaata
    46981 gtatataaac tttctgagaa aaaattagag aaaaataatt aaataaatag ataaagcact
    47041 tggatattat gttgtttcaa gtgctttatt tggtataaaa tggtataata aaaatatagg
    47101 aattttgcag tgttcgattt ttctacttaa gtatagttta acaattggaa tacaaggcat
    47161 tgagaaggtt tgataagtgt tatcaattgc actattaccc gctcactgca aatttgagaa
    47221 agttatatgt gtgtaagtct tggaaataca caatttattt tggggtttta tattaactat
    47281 gtggtatgta aatcttttta aaaaagcacg tcaattacaa tactaaaatt gttttatatt
    47341 aactatgtgg tatgtaaatt tgaaaactgc atgttcttta acaaaagcat ctaattgttt
    47401 tatattaact atgtggtatg taaatgtatt actcccaatt atgaacatag gatatagtct
    47461 ttgttttata ttaactatgt ggtatgtaaa ttatagtttg ccatatgttc ctggttcgta
    47521 tgattggggt tttatattaa ctatgtggta tgtaaataaa tttaatatta ctggaaaagg
    47581 agaaatggat atcggtttta tattaactat gtggtatgta aattttgtta taagaatatc
    47641 gccaccttcc ttttctggtg ttttatatta actatgtggt atgtaaattt ttctgcatct
    47701 tctactatgt tatattcttc taatggtttt atattaacta tgtggtatgt aaatgcacat
    47761 ttaacttcaa gtcttagagt attatctgca agttttatat taactatgtg gtatgtaaat
    47821 ttcgttcaac ttttatctta gcttctcctg ctacttgttt tatattaact atgtggtatg
    47881 taaatgtact agaaaatgca ctacctgcat tcattcctat acggttttat attaactatg
    47941 tggtatgtaa atgtgctatt tccaactttt ttttcaagtt ctgaatactg ttttatatta
    48001 actatgtggt atgtaaatta gttagtccca tatcgttatg gtactgcatt aacgcgtttt
    48061 atattaacta agtggtatgt aaatagtgta aaaggcattt tttcaaattg tgcattggtt
    48121 tggtttatat taactaagtg gtatgtaaag gctaatgttt gagtatttaa aagcatctga
    48181 gcaaacaatt ttatattaac tatgtggact taaaattaaa aataattcaa aaacacttac
    48241 aaatgagtaa gtgttttttt aatgaaagga ggtgataata atgtaaaaat tttacgtata
    48301 tagtataata gtgctataaa ataagtatta tgtgaggtga ttgttgtgtt ttgttcaaat
    48361 tgtggttatg agataactgg tgcaggcaaa ttttgctcaa attgcggaac agctacatta
    48421 gcagataaag ttaacaatga tgatttattt ataaatgttc atggaaaaga attaaatctg
    48481 actaatattt ataaagaaac taaaggagat aaaatcttag caattgatat tgcaatgaag
    48541 ttactagggc tggacataaa agagtgtaaa aatattatat atccagcttt taaagaatta
    48601 agtgaaaaaa taaatatcga agaggaaaaa gagatattga gggaagatga gtataaacaa
    48661 actaatgtac ttgaagatga tgttgctcgt tgccctaggt gtggctctgt ttcattgtct
    48721 gctcataaga aaggttttgg cataggaaaa gctgtagcag gggctactat agcaggaggc
    48781 attggtttag tagctggaaa tttaggagca aagaaagtta gagttacatg tttgagctgt
    48841 ggtaaacagt tttgggcata aataataaac acttactaat gtaggtgttt ttttatatgg
    48901 aaatttatga aaggagagtg aggaaatggc tacaatacaa acttcaataa agattttcga
    48961 cggaatgaca ccagcatttc gtaatatgac taattctatt aatacaacaa ttaatagttt
    49021 agagagattg caaggcagat tgaataatcc actcaatgca ggtaatatac aagcttctca
    49081 acaaagtttg aataacatag aaagtattct cacaaggata gaacagaaaa ttggaagaaa
    49141 tacaaatgaa caggaaaact ttaataataa aataagacaa ggtagtgaag caggttctct
    49201 attagtgtct aaattaaaaa gtattgctgg gatatacatt ggaataaaag gaatagatag
    49261 tattacaaaa gtagcagata caattgcaag tacaaaagca cgtttaaatc taatgaatga
    49321 tggcttacag acaacagacc agcttaataa aatgatttat ttgtcagccc aaagtgcaag
    49381 agctagttat gcagatacag cagcacagat tgctaaactt ggaatacttg caggagatgc
    49441 ttttggaagt tcagcagagg tgataaagtt tacagaactt atgaataaag cttttgtaat
    49501 tggaggaaca tcagcaaatg aagctagtgc agcaatgtat caattaacac aagccatggg
    49561 tgcaggaaaa cttcaaggtg atgagttccg ttccataatg gaaaatgcac ctttattagc
    49621 cactaaaata gctgatgcaa tgggaaaaac taaagaccaa ttgaaggaat tatcaagtag
    49681 cggagcaata acagcagatg ttataagaaa tgcactgttt aaagcttctg atgagataga
    49741 aaagaaattt gcaagtatgg caatcacttt ttctcaagct ctcacaatga tgaaaaatga
    49801 tgcttatatg atattttctg agactctcag taagataagt ggagctttgc aaagtgtacg
    49861 ttttagtgag attgttgtat ctatgcggaa tgttatgatt gcaatatctt caaacattta
    49921 tgatacatta aatattataa aaaatatatt aaatagtgat tttttttcag attttgttag
    49981 caatgttaca ttaggagcta tactaattat caatggactg ggatgggtta ctaatgcaac
    50041 actaaatgtt gctaatgtct ttgctcaaaa ttggtcgatt attgctccta ttatttatgg
    50101 agtgatagca gcaattgcga tatataaagg agtattactt gcaagtacaa ttgccactac
    50161 ggtagcatct tttgtaaatt cattgtatgc agtagcagcg tataaatctt gtgcagcttt
    50221 agcagcacaa gaatttgcga tatttggaaa aatatctgca caaacgatgg aagctttggt
    50281 aacagcacaa gcaacagcag cacaatgggg ttttaatgca gcattattat cctgtccaat
    50341 tttttggata atagcaggta ttatagcatt tgtagtagta gtttttgttg cagtagcagc
    50401 agtaaacaaa ttctcaggaa caagtctgac tgtactagga gcaattgtag gtgcagtatt
    50461 tgcagcagta gcatttatac aaaacataat gatatggcta tttaatagat gcgtagatgt
    50521 aaatgaaggg attgcaaatg gatggaatca atgtgtatat ctaatgaaac aagctattgc
    50581 aaaaggtgta atctttataa ttgagaaaat ggcatcatta aatgatgctg taaataatgc
    50641 tggaaatgca cttgggaaag ctttcataga tggggcaaat atcgcaatac gaggtgtaaa
    50701 caaattaatt gacctaataa ataaaatacc agggataaat attggtaaag ttggagaagc
    50761 aacatttacg cctgtcaagg cagataatag ttacatcaaa caacagattg acagtttaaa
    50821 taaatgggta ggagatgcac cagaaaaaat aaaattggat agaatgggat ataaagatat
    50881 tggagcagaa tttcaaaaag gaaatgcact tggaactaaa tggcaaaatg ctataactga
    50941 taaatttaaa gatacttttg acattaataa gatgctagaa gatgcaaaga aaaaattagg
    51001 attagacgat ttgtggaata aacaaaatcc tttaaacaac cttggtggat ttggtggaga
    51061 tttaggaaaa aatgtaaaag acacagcagg aaacacagcc aaaatggcta aaacaatgga
    51121 taaaagtcaa gaagacctta aatacttaag agacattgca gaacaagaaa caataaacag
    51181 attcacagga gtaaacataa aaattgatat gaacaatact aacaatataa gtaaagacac
    51241 ggatgtggat ggtatagtaa atgttttaac agaaaaactg aatgatgcta tgattgtatc
    51301 agcagaagga atagtttaga aaggagggat ataaatggct tatgactttt atttagatgg
    51361 agtacaatta ccaatacctc cgccaaagtt agagattaaa gttacaaata aaaacaagat
    51421 agttgatttg ataaatactg gagaagtaaa catactaaaa aaagaaggat tatctgaaat
    51481 aagttttgaa gcagaattta cacataataa gttgccattc tatcgtggag cttttaggga
    51541 tgttcaattc tttttaagta aactggaact attaaaaact gattgtaagc catttcaatt
    51601 tattgtatcg agggaattag gtaataaagt actatttaac actaatataa aagtatctct
    51661 tgaagagtat gctatttcag aagatgcaga aaatggttca gatacaaaag ttgcaataaa
    51721 gttaaagcaa tatagagatt actcaactaa aaagttagtt cttgcccctc ctaaaaatga
    51781 gactggtaga cctaatgtaa agatagagcc aaaacgagtt gattcagtca atgccacaaa
    51841 cactaaaaca tatacagtaa aagcagggga tagcctttgg tcaatttgtc agaagcaact
    51901 tggtaatggt tcgttataca agaaagtata tgagttaaat aaaacaatga tggataaagc
    51961 taacaagggt aaaaaagtac ctaaatatac catctataaa gggcaggtgt tgaggcttgt
    52021 ctgatgattt agttctggcg aatgatagag atataaggct agttattgca cattgggaag
    52081 atttctacga acctgctgtc attgatggta tcacatggga gatagaaaga agaggttcac
    52141 cttcaaagtt agagtttact atagcaatgg atgatatatt agagttttgc gaaggtaatt
    52201 ctgtaaggat atattataag ggaattggca tattttatgg atatatattt cagaagaaaa
    52261 gagataaaga aaatcacatt aaaatagttg cttatgacca gttgagatat tttaaaaata
    52321 aagatactta tgtatatagt aataaaacag caagtgaact tgtaaagatg ttggctaaag
    52381 attttaattt aaaatacaat gtcatagaag atacaaaata taaaatatcg agagttgaag
    52441 aaaataaaac actctttgat atggtcttaa ctgcactaga tgatactcta agagagaaaa
    52501 aagaaatgta tgtgttatat gatgattttg gaagaataac attaaagaat gttgcatcaa
    52561 tgaaattaga tactgttatg aacaatgatg taatagaaga ctttgactat aattcatcaa
    52621 tagatagtga tacttacaca aagattaaac ttgtgagaga taatgaagaa acaggaaaaa
    52681 gagatgtgta tattgctcaa gactcaacgc atatgaggag ttggggaata cttcaaatgt
    52741 ttgatacagt agacaaaaac atgagtgaag cagagataaa acaaaagtgt gatatacttc
    52801 taaaactata taataagaaa actaagtcat taagtttaaa aaatgtactt ggagatatta
    52861 gagtgagagc aggttgttta gtacctgttt ttttaaatct aggagatatt aaacttcaaa
    52921 attatatgct agttgagaaa gtaaaacata cttttgaaaa taacagtcat tttatggatt
    52981 tgactcttgt tgatggagat gaatttgctt cttattcttc aagctcatat tcaagtggaa
    53041 atactaacaa taaggatgaa aagaaaaatg gtcctgcaca aagtattacg aaaaaaaata
    53101 caggtaaaaa agttcctgct atatttactg catattatcc agggaacaat gcaatggaag
    53161 gcggaaaaac agattgcaat ggaaagccac ttgatgtaaa atcaagaact gttgctggtc
    53221 caatgaatcg agaaggagtt aagaaaactt ggtatactga tgattttcta aagaaacatc
    53281 cagtttttga atatggagat aaagtaaaaa ttatacttcc tggtactgcc tatgacaaca
    53341 aagtatatac agttaaagat aatggaggaa gaatatatgt tgaaacaaac ggaacatatc
    53401 atatagatgt actattagct aatgctagtg aatgtaaaaa atttggtaga aagaatggct
    53461 atataattat aggtggagat gaagaacaaa catatcaagt tgaaggtaat aaccaaagta
    53521 gtacaaataa taactctaaa gaagataaat taattagtat agcaaaaagt aaactgggtt
    53581 gtaattatgt gtatggagca gaaggtccta ataattttga ttgcagtggg tttactcaat
    53641 ggtgttataa acaaataggt ataaaaattc ctcgtactgc ttctgcacaa agtaaagcag
    53701 gaaaagcagt agatttaaaa gatagaagca agtggaaagc aggagactta ttatgtagaa
    53761 ttggaggagg aagtagtaat catgttgtaa tgtatattgg aaacaatcaa ataattcatt
    53821 caccacaaac aggagatgtg gtaaaaatag agtctgttaa ttcttataga aaaggaaaag
    53881 catacacaca tgtgagaaga tttatataag tgaggtggca atatgagcca agatttatta
    53941 cagataataa aaaaagctgc aatggatgca gtagaaacaa gcaacccaat gagggttgta
    54001 tttggaacaa tagaaagtat tagtcctcta agagttaaga tagaacaaaa actatctatt
    54061 ggtgaaattt ttctaataca aacagataca tttaaaagat atacagataa aaaaatagga
    54121 gataaattag tcttaattcg tatgcaagga gggcaacaat acttgatttt agataggatg
    54181 tgataaagtg ttaccaagtg ataatttaga ttatgatatt gaagatgtat caataattaa
    54241 ttttgatgta agacaagagc caagtaagac atttaaactt catatagaaa agtctaagat
    54301 tgatggtatt tgtgatgatg tagaagcatt aaaacagacc atctttttga ttttaaacac
    54361 agagagatac caacatctaa tatatagttg gaattatgga gtcgagttga acgaccttat
    54421 tggagagcct atatcctttg taataccaga gttagagaga agaatcaaag aagcactaat
    54481 tcaagatgat agggttgaaa atgtagataa ttttgagttt gaaaatataa agggtaaagt
    54541 acaatgtaaa ttttcagttc atacaaaata tggaaatata aaagcagaga aggtggtgag
    54601 tgtataattg tttgagttaa tgacatttga aaatataatt aaaagaatgt tagatagtgt
    54661 acctaacaca cttgataaga gagaaggttc tataatatac aatgccttgg caccagttgc
    54721 agtggagctt acagaaacat atattgctat ggatgaatta ctagaccaaa cattcgtaga
    54781 tactgctagt tattactatt tagagaagag atgtaaagag cgaggaatta cacctttacc
    54841 agccactaat acaattgcta aaggagtttt taacatagat attccacttg attctaggtt
    54901 taatctagga gaatacaatt atattgcaat tgagagaata tctgaaaaaa catataaaat
    54961 gaaatgtgaa actgctggac ctatatttga gttaggaaaa ctaataccta ttgaatatat
    55021 agatggtctt gaaactgctg aactaactga aatcttgata aatggagagg atgaagagtc
    55081 agaagatagt ttaagacaaa gatattatga tagcctaaat tcacagagct ttggtgggaa
    55141 tatacaaaac tataaagatg aagttaacaa aatacaagat gttggaggag ttaaggttta
    55201 tcctgtgtgg gacggtggag gaactgttaa gttagtaata attaactcta atttcaaagt
    55261 accatcagag gatttagtta atttagtgca agaagaaatt gacccaattg gacatcaagg
    55321 acaaggctta ggattagcac caatagggca taaagttact gttacaggtg ttgtaagtac
    55381 aactataaat atatcagcag agataacata caaaaatggc tacacttggg agaatataaa
    55441 atcaattgca gaagaagcaa tagacgacta tttaaatgaa cttaacatga gttgggaaga
    55501 tgaagaaaac ttaatagtcc gtatatctca aattgaaact agattactta gtatagatgg
    55561 agtgttagat attacaaaca caatgataaa tgatgttaaa tctaatctaa caatagatag
    55621 taacagtata gtagtgagag gtgaggtagt tggataaaga gattaatcta ataaattact
    55681 taccacaaat tctacaagat aaagaagaat atataaaagt atttaatgta gaaaacaaag
    55741 aaataaaaac actacatgaa aaattaaatg acctatcaag tgaccagttt ttagaggatt
    55801 taactccaag tggtataaaa agatgggaaa agataatgtc tataactcct aaaagtaatg
    55861 agagtttaga agatagaagg tttaggattt ttagtaaata tataagtaaa ctaccttact
    55921 cagagagatt tttaaggaac tggctagata gtatagttgg agaaggcaat tatgaattaa
    55981 ctattaataa tgctacttat aatatacatc ttgaaagtga tgctagaaat caagattggt
    56041 ttgaggaggt tcattctttt gtaagtagta ttaaaccttg caacatgact ttagattaca
    56101 ctagagtgct tgtaagtaaa gacaattata tgaattttgg tataacaacc ctaataggtc
    56161 aagaaataac tatataccct tggagtccac cagatataga aacttatgga gaaattgatg
    56221 tattaactgg caatggagtt ggataccaag agataacaat attttaggag gtgatatatt
    56281 ggctatagat aaaagttatt acactataat tacagatgta gggaaagcaa agatagcaaa
    56341 tgcaagtgtc acaggtaata aagtgggatt tgtaaaaatt caacttggtg atggaggagg
    56401 gagtgaatat actccaactg agagtcagac agctctcaaa aatgtggtat gggaaggcaa
    56461 tatcggaaat acaactacag atgaaactgc accaaattgt ataatattag agagtttaat
    56521 accatcaagt gtaggcgggt ttatgataag agaaatagga tatttagatg atgaaaataa
    56581 tttaattgcc atttctaaat acaaagagtg ttataaacct tctatagaac aaggtgcagt
    56641 ggtagacatg aaggttaaaa ctgtgcttat tgtatctaat gtaaataata tagaacttaa
    56701 aattgaccca acaataatct ttgcaacact caaagatata caagacttag aaactaaaat
    56761 aggtactgtt aatactaaaa ttgatacaac taaaacagaa ttaacaagca acatagaaac
    56821 tgctaaaaca gagttaaata ctaaaatcga ccaattaatc gcaggtggct caaatgttgc
    56881 atacactcaa agggtcgcaa ttgatgattg ggttgaggat gcagaaagtg gattcaaagc
    56941 aactgtaaca catagtttat taacacagag aatagttgta aatattatag atgctactac
    57001 aaaagaaaat atagttccaa attttaaaat agttgatgat aattcaattg agattagaag
    57061 tgaagtaaaa gttgagttaa atgtctatgt gataaatgga aatgcagaaa ctcattttat
    57121 aaatgcaact gtagatgata acagagtatc tgaaatgact acttattcat ctaagaaaat
    57181 agaggacaga ttggttaata tagaagaaaa ggtaaatggt ggtttatcta atattgcaac
    57241 aagtgtaaat gagttgataa cttattgtta gagaggagag tgagaaaatg cagactgaat
    57301 ggaattttaa ttatgctaat tatgtacaaa atgtttcatt gccacctggg cgatataaat
    57361 tagaatgttg gggtgcttgt ggtggtgctg tcgatacaag cgattggact gattgtgcaa
    57421 aaggtggtta ttcaaaaggt gagattgtat ttaaaaaaag aactaatcta caaatttgtg
    57481 tcggtcaatc cggttatgag aaagtttctg aaggttcaag ccttactaga agtggtttta
    57541 acggtgcagg cgctgctggc aaagttacta ctggtagctt tgcttattct aaatacggtg
    57601 gtggagcaac tgatataaga ctttatcatc ctagtgcaac ttggggtaac accgaaagtt
    57661 tgctttcacg catacttgtt gcaggcggtg gaggaggtat gaaaaataat tttgcttctg
    57721 ctcgttctat tggtcatggt ggtggttatg taggtgttaa tggagttggt cgtgacagag
    57781 atttttgtgg cggtggttct caataccaag gtggaacaag ttacgacaca gaagaatacc
    57841 atggttcatt aggaaaagga ggttatggta acataggaat aggtggtgga gggggttggt
    57901 acggtggtgc tggttcttat tctaatgaat gtggaggtgg tggaagtggt tacgcactaa
    57961 ataaagatag ttataaggta cccggatata taccaacacc tgaatattat cttgaaaata
    58021 tagtcatgac tactggaggt aatactacta aagcagatgg ttatgctaaa ataacattac
    58081 tacaagcatt gccattttta acagtatctt cttataattc tacacaggca acatttaaag
    58141 ccgaccacac agaccctgca ttacttacaa agatagaatg gtttatagat gatgtactaa
    58201 aagaaactat aacaacaaat ttaacagaag agaaaacaat taattataca ttagaagata
    58261 atgcattaca cacacttaaa atagttgtta cagacagtaa taatgctaca gcagaaaaag
    58321 tgttaagtat aagtaagaat ataatgccac tgcccgaaaa tgtaaattta aatgatatat
    58381 caacaaaatt agttgaggtt aatgcaggat ttaaagttgg gaaaacaagt attataaaca
    58441 ctttagcact taaaaatata gaagcaagtt taaataatac acttgtggag ttatcagaga
    58501 aaataaaaac aagttttgat agttcagaca ctagtgtgca agatttacaa aatcaagtaa
    58561 cacaaaaaaa caatactata actcaattag aaacagagtt aagtaaaaga aaaagattta
    58621 taacaggaac ttatacattc acaaaaacgg atgcagagaa ttttaactta agtatatatg
    58681 acaaagaagg aacttcaaaa actcttacta taccagttaa catgggcttc tctcctagtt
    58741 taattgtact tagtggagta acttttagta caacaagtaa atcttatgtt tattttgata
    58801 atgtttgtaa ttcaaatttt tataattttg gctataattc tgattctact catagtaacc
    58861 cgaaagctgt tggtattctt aatgtatcta atgttgggta ttcttcatta gtgcttacat
    58921 tatataaatt atcaatgtca gaagcagttg gaatatgggc aaaagaggga gctacattaa
    58981 cttacaaaat atatatttaa taaaaaaatg aggtgataat atgaacagaa ataatagaat
    59041 aatttacgac caaacaggca atatatggct tcaaactggt gaagcaacag gagatataca
    59101 agagtggtca aaaataactg aattaaattt tttggatgtt gaatttggta gtatagacta
    59161 tagtaaacaa tatatagagt ctataaatcc agttacaaaa gaaccaatca tcaaagacat
    59221 agaagtcatt ttaacagatg aacaaaagag attacaagca ttagaaaaag aattaagcat
    59281 gttaaaagaa gaaaataaaa atagagatag tgagatagta aacacagcct ttgaggtaga
    59341 aaatataaaa ttaaataaca atttatagga ggaattaaca tgtataactt attaaaactt
    59401 atgatagaac aaaagaacta tagcactaaa gaggatttgc aacacaaaat tgatgtattc
    59461 tatgcagtaa atagaattac agaagaacaa tatttagaac ttacaagttt attaaataaa
    59521 gaagaaacac cagcagaacc aataaaatag gttctttttt tattgaaaga aggtgactaa
    59581 atgactttta aagagttagt taataaagtt agaaatcttg tattagaagc aaagaatgta
    59641 actatagaag atacagaaag taaatttaca agtgaaaatg tagaaggagc attgaaagaa
    59701 tgtatagata gagcagatga ggcttttcaa gaagccgata gtggaaaaac acttttatca
    59761 actgctatcg gctctcctgc tacatcagaa caaacatttc aagattatgc gaactatatt
    59821 acaggattta agagcaatat aagcaattta gaaactcaat tgaaaagcaa atattctatt
    59881 agatatggtc ctattgatgg atatgatggt aatccttttt ctgctaattt tggcaagagt
    59941 gcaagttacc ttattgtcta tgtttacttt agaagaagtg tatattatta taatcccagt
    60001 ggtagttctt taggaagtaa tacaggaggt tctgaacgtg catggattac tatagacagc
    60061 aataaaactg gtttttcagt tcattcatat gatactagtt atgagccata tccttttaca
    60121 ggttattata ttgcttgttt cgcataataa attatattat taaaactagg aggatgtatg
    60181 gaagaaatta gcataaatct attatgtgca gttgcaggag ttgtaatatc ctacttagca
    60241 tttagaaata gctcaaacag aaagatacaa gatgatacag aaacaactac aaaattagaa
    60301 caacaaataa cttttctgtg tgagaatgta agagatataa agcatgatgt agcaaagttt
    60361 aatacaagtt tcttagatat cagtgaacga gttgcaaaag tagaagcaag tacaaaacaa
    60421 gcacatctta gaattgatga aattataaat agaattggag gaaaataaaa gatggataat
    60481 ttaataagtt ttataccaga gcagttacta attttagtag ctgctcttta tgttatagga
    60541 gcaggttgca aaaaatataa acaattagat aataaataca ttccagtagt gttattgata
    60601 cttggaatag gtttctcaat atggatgcta ggattaaatc ctgttgcagt cttacaaggt
    60661 gtaatttgtt ggggagttgc aataggtgta aatcaaactt acaaacagtt taaggatggt
    60721 gaaaagtaat gaaattaaca aaaatattat tactcacaaa aatttaaaaa atttcattaa
    60781 atagagtata cttttatagc taataagtat ataataatag tacagaactc gtaaagcatt
    60841 tatatgctca aatatacggg acattgattt ttgaaggaac ccgccaagct tcttttatag
    60901 ctcaaatagt cgggacagat aaatttaccc actaatatta attggtgggt tttattatat
    60961 aaaagtatat ctatgaaaag gtgaaaacaa tggttgaagt aaaagaagaa aaaacatttg
    61021 atgaacaaat agatatttta aaaagtagag gattaataat aaatgataaa gaagatgcta
    61081 aatttgtatt aagtaatgtc aattattata ggtttacagc atatcttcta agttttaaga
    61141 atgatgatgg ctcatataaa gaaggaacta cgtttgaaga agtttatgat atatataggt
    61201 ttaataagga atttaggata ttattaacag atttgttagg gagcatagaa atagcattta
    61261 ggacatacat tgcatataca ttagcaatta aacatggtgc ttgtggatat ctataaaggg
    61321 agagtttcaa agatgaaaaa ttctatatta attttttgac agcattagag agagaaaaaa
    61381 ataataattc agataagctt tttattatac accataaaga aaaatataaa ggaaaacttc
    61441 ccatttgggt tgcaacggaa ataatgactt ttggtatgtt gtaaaaatta tattcaaaca
    61501 tgttgccaga agatactaga tacataaaaa ataatttgtg tagagtgaat actttattag
    61561 ttaaatcttg gttacaatca ttaacacagg tcagaaatca atgcgctcat tatggtagaa
    61621 tatataataa taatttccgt attataacaa taaaaaacga atataaaaag tataacttgg
    61681 ataataaaaa gatattttct tatatacttg ctatgaagca tttgactatg gataaattaa
    61741 tttggaatag tttttttata aaacttcaaa agttaattaa tgattataat aattctatag
    61801 acttaaagct tattggtttt cctaataatt ggatagagat attggctaaa taaaatagtt
    61861 actttaagaa gtttataaac acttactata tgtaagtgtt tttttattga aaagaaggag
    61921 gaaaataaat aatgaaaata tgtattacag taggacacag tattttaaaa agtggtgcat
    61981 gcacttctgc tgatggagta gttaacgaat accaatacaa taaatctctt gcaccagtat
    62041 tagcagatac atttagaaaa gaaggtcata aggcagatgt aataatatgc cctgaaaagc
    62101 agtttaaaac taaagcagaa gaaaagactt ataaaatacc tagagttaat agtggaggat
    62161 atgatttact tatagaacta catctaaatg caagtgatgg tcaaggaaaa ggttcagaag
    62221 ttctatatta tagtaataaa ggtttagaat atgcaactag aatatgtaat aagctaggta
    62281 cagtatttag aaatagaaga gctaaattag ataaaggatt atatatctta aatagttcaa
    62341 atcctacagc agtattaatt gaaagtttct tctgtgataa taaagaagat tatgagaaag
    62401 ctaagaaact aggtcatgaa ggtattgcta agttaattgt agaaggtgta ttaaataaaa
    62461 atataaataa tgagggagtt aaacagatgt acaaacatac aattgtttat gatggagaag
    62521 ttgacaaaat ctctgcaact gtagttggtt ggggttataa tgatgggaaa atactgatat
    62581 gtgatataaa agattacgtg ccaggtcaga cgcaaaatct ttatgtcatt ggtggtgcag
    62641 catgtgagaa gattggttct atgactaaag aaaaatttac tatgataaag ggtaatgata
    62701 gatttgatac actttataag gcattagatt ttattaatag atagattata taaaatattt
    62761 tagtatttct tgaaaatcat aagtatggta atatttagat aaatattatt tattattaat
    62821 aggtatgggg gtagattatg atatttaaga agtatataaa atcattactg atagttgtta
    62881 gtgttacatt gataatgaac aaatcaatat tatttgtaaa tgcattagat ttagagagta
    62941 acaataacat aaaatcttat aaaagaaaga atgtaaagga aattgatata agcaataaat
    63001 ttgttgatga atcctttaga agagatattg ttgaaaaggt attagataat agggatgatc
    63061 cattaacaaa tgaagttgca tcaaaagata actataaaat atatgaaagt gatttaaata
    63121 aaattgagag ttgcttagag ttagatttac ataaaaatcc agatgtttcc aacccaaaca
    63181 aaatagataa tttagatggg atagaaaatt ttagaaatct agaaaagctt gattgtagtt
    63241 caaataatct agaaagtgta gatttaagta aaaatataaa attaaaatat ctagatatta
    63301 gtggaaataa aatattaaac attaatttaa aaaacaataa tcttattaat gaattacatt
    63361 gtgatgcaaa taaattatca agtttagacc ttaaaaataa taaaaatttg gtaagtttat
    63421 tttttactag taatgaatta acagagatag atttaagtaa taatgtgaac ttagaaaatt
    63481 tgcaatttct taataataat ttaaaaagta tagatttaag tcataataaa aagttgaaat
    63541 ctctaaattg tactaacaat aaacttaaat ctcttaattt aagtaataat gtttttttga
    63601 aaaatttaag ttgttcaggt aataacctaa gtaaattaaa tataaaagga cttacaagtc
    63661 ttgagtattt gggatgtgct aagaataaat tacaagattt agatgtatct acaaatataa
    63721 aattagattc tttattttgt gggaaaaata gtttaacaaa aatagatata agtaaaaata
    63781 aagaattaaa atcattggaa tgtagtggtc aatatataga aaaaagtatt ataaaaaatg
    63841 gtgaaaatat agttgtagat ataggaattg aaaattcgaa attaaaggaa ttatatccac
    63901 aaaattataa tatctcaaca ggttttctaa catggaaaaa tatttctgaa tttccaaata
    63961 acgtaagtta tagtttggaa tatgaatatg gtccagtaaa tgatgtagag actatggatg
    64021 taaatatatc aatagataaa ttagttaaat gtagaatcag atatatagat tatgatggaa
    64081 gtataatcaa tgaaaaagaa atatcagata attctagtat aaatgaaatt caaattaatc
    64141 ctcaaagaga aaactatgag tttaatggtt ggagaaacaa taaagatgaa aaaatatata
    64201 catcagagca ggtaagtaaa ttagtttttg atgatagtac tgattttata gcaacttata
    64261 aaagaaaatc aacaggatca agtggttctt ctggtggaac ttcaagtaaa gaagatttta
    64321 atatagcacc atctaaggaa aaattaacag gtgcagacag aaatgaaaca tcagtgaaaa
    64381 taagtcaaaa aggatggaat aaagctgata atatagtgtt aataaatgac tctagcatat
    64441 cggatgcttt atcagcaaca ccatttgcta agtctaaaga tgcaccaata ttacttacta
    64501 aaaataataa cttaaataaa ttaacagaaa aagaaataaa tagattagaa gcaaaaaatg
    64561 tgtacatagt gggtggatta aagtctgttg atgaaaaagt agtgtctgat ttaaagaaaa
    64621 aaggattaaa tgtaattaga atatctggaa atgatagata tgaaacatca ataaaacttg
    64681 caaaagaatt agacaaaaat tctaacctgt caaaagtagt agtggtaaat ggagaaaagg
    64741 gactagctga tgcagtaagt atgggagcta tatcagcaaa agaggaaatg ccaattcttc
    64801 ttactaatca aaatgatgat atgaaagata taaaagactt aatagctaat aaaaacatat
    64861 caaaatcata tgtaataggt ggagaatctt tatttaataa taaagaagtg aataacacat
    64921 taccatctgt aactaaaata gcaggttctg atagaacaga aactaactca aaagtaataa
    64981 gtcatttcta tagcaaagat acacttaatg acttatatgt agctaagaat ggaatgaata
    65041 aacaagacga tttagtagat gccttatcag taggagtgct tgcaggtaaa actgaatctc
    65101 cagtagtatt agttggaaat ggattagatg atagccaaaa agagttaatc aagaataaaa
    65161 agtttaaaaa tataactcaa attggtggaa atggaaatga aaaagcattt aatgaagtag
    65221 aaaacttagt taaatagatt gaaaggtagt aactggaaat agttactacc tcctttttta
    65281 ttcattttca gttttttcaa taattatttt tccatcctca aaagtaacaa gaacatccct
    65341 ttcatcagga gagatattca tctctttaat ccatgacatt ggaagtatca atctgggtgt
    65401 aaaagagcct ttgccacttt tattgaatgc aactctaagt tttctttgtt ccaataatat
    65461 cattcctttc tactattaat aaatgccagc aataataaag taaataccaa agcaataaga
    65521 gctttaaaag taaaacttat tattatatta ataatattta aaactatagt gataccaaga
    65581 ataatcatac aaattctata aaatttatta ttcatattaa attagaaaat gtgttataat
    65641 attagaaaga agctacctag aaggaaggta ttttcctcct aggattgaaa cttataattc
    65701 tttaatcaat cttagtattg cgagtactag attaattaaa actaatgtga aagagaaagc
    65761 atttttaatt ttattaaatt tgttttctct tttatttttt cttctttcta atctccccac
    65821 tttctttgtc ctcccttcta agttttttat tttcttacct cctttctata tttatattat
    65881 atcatcacgt gacgtgaaag tcaataaata ttccatattt ttcaataaag ctataaaata
    65941 tattaatata aaatacaaaa tataataaaa ataagtggat gtttacatta tacacccact
    66001 tattttagag ttattcctca tcagattttt ttattataat ctcatcttcc ttaaaaataa
    66061 ctacagcctt acgtttttcc cttgtaaatc ccattttttc aagttggctt aaaggtaaag
    66121 aaattttagc cgaataagta cctgaaccac ctttactgta aactatttta gattcttttt
    66181 gctccaaact attttcctcc ttttttatat tgtaatgtaa caaatattaa caataaaatg
    66241 cttactatta atcctattat attgttgata ttaaaatgta aaaataaaag tattatgtct
    66301 attactatag ttatagcaac tataataaga cagaatttat aaactttatc actcatattt
    66361 tatagaaaat gtgttataat atttagaaag aagtcactag gaagaagatt aaattcttcc
    66421 tagcttacaa aactataaat cttttaataa tttaattagt ttatcaatta aatttattaa
    66481 aatgggtaag atagagagag cagttttaac tttggcgagt ttgctttctc tatctttttt
    66541 tcttctttct attctactca ttttcctttt cacccccttt ctatatttat attataatat
    66601 gccgaaccgg caaaagcgat atttatttcc atatttttca ataaagttat attgacaaac
    66661 tatatttttt tacaaataaa aataagggtg gcaaaaccaa acttgccacc cttttgccac
    66721 cgaaaataaa taatagaata aatttaaatt aaacataata aaacataaac aacaaaactg
    66781 accttaataa aacattgaaa taccaacact tgtaaaaaat aacaacaata aacaaataac
    66841 ctagaataat attacaactt aagtaaataa taattttgaa taataattta cggaggtatt
    66901 catatgttta aacacgataa agcattatta aaagaagtaa aagtagaaag accaaatcct
    66961 caatatgctg ttttaatgca agaacagttg ggtggtgcaa atggagagtt aaaagcagct
    67021 atgcaatact tgtcccaaag ttttagaata aaagaccctc aaataaaaga tttatttctt
    67081 gatatagcag cagaagagct tagccatatg gaaatggtag cacaaactat aaatttatta
    67141 aatggacatg atgttgatta taattcagtt aatacaggcg aaatagaaac acatgtatta
    67201 actggtctat caccagtctt aataaactca tcaggagctc cttggactgc taattacgtt
    67261 acagttacag gggatttagt agctgactta ctttcaaata tagcttctga acaaagagct
    67321 aaggtagttt atgagtactt gtatagacaa atagatgata aatatgtaaa agaaacaata
    67381 gatttcttac ttaatagaga agaagcacat aatgctctat ttagagatgc tttaaataaa
    67441 gttaaagata caggttcaaa tagagatttt ggagttacag aagattctaa attatatttc
    67501 gatttgtcta ctccagggcc aaatcacgat actaagatag atattaatcc tccttctttt
    67561 gaaaaacctt taaaaaaata atttgatatt ttttatgttg ttttgtttta gtagttataa
    67621 aaattattta ctaggagaat taattatatg aaaaattaaa tatgtatggt taaaaatggg
    67681 tatacactta ttataagtgt atatctattt gtttattgca aaacatataa taaaataaca
    67741 aatgaggtgg tattttggac agtaatagaa aacctaataa tctaataaat gaaaaatctc
    67801 catatctttt acagcatgca tataatccta taaactggta tagttggaat gatgaggctt
    67861 ttaaaaaagc aaaagaagag gataagccaa tatttttaag tgtaggatat tcaacatgtc
    67921 attggtgcca tgttatggaa aaagaatctt ttgaagatga agaagttgct gaaataatga
    67981 atagaaactt tgtggcgata aaagtagata aagaggaaag acctgatgta gatagcgtgt
    68041 atatgacagt ttgccaagcc atgacaggta gtggtggatg gccaatgacc ataataatga
    68101 caccagataa aaaacctttt tttgcgggaa catatttccc aaaatattca aggtataata
    68161 gacctggagt tatagattta ttagagaatg tatctgaaaa gtggaataca agcagggata
    68221 tattaataaa atctggagat gaaataatag aagctcttaa ggatgatttt ggtgtaaaaa
    68281 atactgaggg tgatttatca aaagagatgt tgagttcatc agttagagta tttaaggcta
    68341 tttatgatga aaagtatggt ggttttggta atgctcctaa atttccaagt cctcagaatc
    68401 ttatgttttt aatgaaatat tatagtatag aaaaagacaa agatgtgtta aaaatggtgg
    68461 aaaaaacttt agatggaatg tatagaggag gcttgtttga tcatattgga tttgggtttt
    68521 caagatactc tactgataaa aagtggttgg ctcctcattt tgaaaaaatg ctatatgata
    68581 atgctatgct tactatagct tttttagatg cgtataaaat aacaaagaaa gagttatata
    68641 aagaaattgc tataaaaact atagattatg tagttagaga gatgaaggat aaagaaggtg
    68701 ggttttattc agcacaggat gctgatagtg aaggtgaaga aggtaaattt tatacattta
    68761 atcctcttga gataattgaa gttttgggtg aagaagatgg aattttcttt aataattatt
    68821 ttgatataac tagtagtgga aattttgaag gcaaaagtat tcctaatctt ataaaaaata
    68881 aagaatatga gagacataat gaaaagatag ctgaccttag taaaaaggta tttgaatata
    68941 gaaaagaaag aacatctttg cataaagatg acaagatact tacatcatgg aatgcattga
    69001 tgatagttgc tttaacaaag gcatatagta ctttaaaaaa tgacatatat ttagagtatt
    69061 caaataagtg tcttaacttt ataaataata atcttgtaaa tgagtcaggt agattattag
    69121 ctagatatag agatggaagt tccgactatt tagcatattt agatgattat gcttttttaa
    69181 tctgggcgta tatagagctt tatgagtcaa catttaatat gaaatattta gaaaaggctt
    69241 taaatttgaa tgaaagctgt atcaaccttt tttgggatta tgaaaaaagt ggattttata
    69301 tatatggtaa agatagtgaa aatttaatag caagaccaaa agatttgtat gatggagcta
    69361 ttccatcagg aaattctgtt caattataca atcttataag acttgcgaaa attactggag
    69421 ataatagact tgaagaaatg tcatataaac aattaaaact gtatgtggat aatgtaaaga
    69481 gttctccaac tggctacagc ttttatatgc tttcattgat gtttgaactt tattctacta
    69541 aggaaattat atgtattttt aaggaagatt ctgatttaat tgcatttaaa gaattgataa
    69601 gtgaaaattt tataccaaat gcaacttttt tagcaaagaa atacaatgaa gaaaacacta
    69661 ttataggttt tttaaataac tatatattga aggatgataa gactagttat tacgtgtgtc
    69721 aaagtaatag ttgtagtcaa ccaattaatg atttacaaaa attaaaagat atgattttag
    69781 gagtagaata atcataatag cgaaaaagga gatgctttaa aataaaagtc taatcatctc
    69841 ctttttttgt ataaaaaaca gaaaaagagt tcctaagaac acctaatcta tgtaatgatt
    69901 gttttgttaa aaaataaaaa tgacattata gttatataaa atattgtact ataaaaaagt
    69961 ggtattatag atgcgatagt attaatcttt tttatttacc ctatttcttc tttttgcaaa
    70021 ttcttcaagt gcttcagatt taattttatc tatttcttca tattccataa aattagcatc
    70081 ttcttcaaag ccatcttgtt cttcaccatt aaaacgatac atttttttta gtaaactcca
    70141 taaggtctct aattcatctt tggtaaattc gtgaaataca tctgccataa agtttattcc
    70201 agtcctacta catgttacca taaccttttt tcctaagtct gtaactttaa cattaattgc
    70261 tctcttatca tgaggacttg gaattacatc aacatatcca tttttttcaa gattagcaac
    70321 tagtctattt atattttgtt tacttgtacc catttttctt gcaatattat ttaatgttgt
    70381 ttcttcctct ggtaaatgaa gaattgataa aatagtcata tactgtcttg aagtaagtat
    70441 accaaaatat ttatctcctt ctatttgtat tttgtttgta agggtaaaaa gagttgcata
    70501 tgtttgattc attaaaaaga gctcttttag ttcatttgaa taatccatga aatatatctc
    70561 ctttattttt agatgttgtt ttgtcgaaaa cagaatatca tgaattaatt ctaatgtcaa
    70621 tatggtgtca aaatatatta aagtattact tttttatgta actattaatt aattaaatta
    70681 ttgtataatt ttatgtaaaa acttctatgt accaatgata atttctatga ggaattaata
    70741 attcttaaat tttttagaaa gattttgact tgaaaatact tgtaatataa tatataaatt
    70801 aatatactga ataaaatcaa tctggaggga taattatgaa gtgtacaaac tttactttca
    70861 aaggagagga aggtttagat atatatacat ataaatggga agatgaaaat ataaaaaaac
    70921 caaaggcagt tatccaaatt gcacatggaa tggctgaaac tgcacaaagg tatgagactt
    70981 ttgccaaggt acttactaaa aatggttata tagtatatat caatgaccat agaggtcatg
    71041 gaaaaactgc aaagataata gaaaatgttg gacatttagc tgaaaaagaa ggatttagat
    71101 gtcttgtaga ggacatgtat actttaacaa atattataaa gaaagaaaat gaagatttgc
    71161 caatttatct atttggacac agcatgggtt catttgcaag tcaaagatat ataatggatt
    71221 acagtaataa tttatctggt cttatactat gtggttcaaa tggaaagcaa ggaatcattc
    71281 taaatcttgc tcatctgata atcaatcgtg agattaaaaa gtatggaaga cgttctaaaa
    71341 gtaataaaat aaataatttg atatttggtg gcgaaataat aagaagaaat gaaaagacta
    71401 agtttgactg gttaagtaga gataaagaac aagttgaaaa atatataaat gacccatttt
    71461 gtggagtagt gtgtagttgt ggcttttttt atgatttagt tcaagggtta aaagaaattg
    71521 aggataaaga aaacttaaaa aaagtgccac ttgatatacc tatttacata atatctggag
    71581 ataaagaccc cataggaaaa aatggtaaag gagtcttaag actaagggat agatatataa
    71641 aattaggagt aaaagatgta acttgcaaac tttacaaaga tggaaggcat gaattgttga
    71701 atgaaataaa tagagaagaa gtttttgagg atataatttg ttggttaaat aataaaatag
    71761 aaatattgta ataaaaaaat ataatgatat atttgcttta aatttattta tatataatat
    71821 ttgaaaagtg gattatgtat atgcaaagta gaaaagagag agcctagaag tgattttttg
    71881 ctataatgcc atttattgaa taggtgggtg ttattataaa taaattatta acactcagta
    71941 tgattatcat tgtatatgga atttatttta tttaaaattc cttgaaagtt taaatatagt
    72001 aagttgtaat aaagagaaaa tctaaatatt taaatgctat ttaatgtgaa tctattaatt
    72061 aggttatact taaacttaat taatagattt tatatttcaa gccaatgaga tagggtttta
    72121 gattaaaata taaccaatgg ttaaattaca gacattttgt taactttccc tcgatttact
    72181 agtaaatatt ttttttatat acttaaatta caacagatgt actagggagg tatatatgca
    72241 aattaaaata ggtaaagtca tacagtgtct tagaaaagaa cgaaatttaa ctcaagaaca
    72301 attagcaaag tttataggtg tgtcaacacc agcagtatct aagtgggaga gtggaaattc
    72361 ttacccagat atagaattat tgcctttatt agcagacttt tttaatgtgt ctattgataa
    72421 gcttttaaat tacaagattg atttaagtga agaagaagta atgaaaattt ataaagaatt
    72481 agaatcaggc tttgctagaa ttgaaattga tttatcaaca gaagaaccaa aagaagaatt
    72541 tagacaagac ttagaatctg taaaaaagct ttccaatatg tatatagaga agtatcctaa
    72601 aagctattta ttgaagctaa ggatatgttc gctgtatcag atgtattcat ataaatttgg
    72661 taagagtgaa cttaatgaca gagtaaaaga aacaatgaat attcttgaag atatagtgag
    72721 aaatacagat gatatccaaa taaaagaaac agctcttatt atattatcaa acgcatattg
    72781 tatgttggag gattatgaaa aggcagagtt gtatttaaat atgatacata agtcaatagg
    72841 ggatacaagt gttaatttag ctatgatata tttaaagcaa aatagacttg aagaagcaga
    72901 aatattgctt caaaataaat tgtttagtaa tgtatttaat ataagcatgg attgtaaggg
    72961 aataattaat gtatacaaaa atcaatacaa ggaattaaag aaaaaattgg agaatagaaa
    73021 ttttaataaa aatgcaattg agagagaaat ggagtatata aaaaataagt tgctggggta
    73081 tgcaaatctt tctttagaaa taaaaaaaat gctcagtgaa gataaaggtg cattttttag
    73141 tatgtatatg gattatatgg aattatctct aattttttta ttttttaaca tgaaagaaga
    73201 agcaaaaaaa gccttatata gtctaaaaga aatattagaa aaatatccta tacatgaaag
    73261 tttggatgta agtcaaatga gattttttga taaggtagag tctaaaaatt tatatacttt
    73321 taatatatat acaaacttgt taattgtact taatgatgat agttataatg agcttagaga
    73381 agaaccaatt tttaaggatg taattgaaaa gattttagat atggaaaaaa tgttgaaaaa
    73441 taaagaataa gtaaaaattt aatataggac ttaaagatta atcaaattaa aagaaagtgt
    73501 ttcaaaataa aaatttgaat cacctttttt gtcggtctta aacagttatt tagaaaaata
    73561 gaggtttcaa aatagaaatc tgaattatct gtattattac acaaaaatag aaacctaaat
    73621 ttataaaaat agaataaagt ttgttatata aaatgtaatt ttgtctataa aatctcactt
    73681 gttttcttaa ctttaagcgt gaaaaagaga atgtatcatg aattttttag ttcatgatgc
    73741 atccttcttt gtatgaaatc caatatatct ttattattta aacaatggaa aagagcctta
    73801 tttctatttt gagacatctc ttttatttaa ttatgaagtt ttatacagtt caataaatag
    73861 gaattatatt tagaaactca tgacattatt tcttagatag tgattaaatt tatcatattc
    73921 cattttagat aacttttctg ggtaagacat tacaatcagg ttatgcccat taaatttttc
    73981 atggtatggt ggatgaatgt gttcataatt atttgtctta tattttactc tttcatagaa
    74041 cgccttgcga taaattgaaa tctcatcaat tggaaggtca atttctaaaa taatagtttt
    74101 tcctttctta tttaaaagct ctaacgctct ttgtccatat ctattattgc gcatttctgg
    74161 aaaaatgcag aaatgttcta catagataaa gtcatctgtt tcccaacata aaattatgcc
    74221 gacaaactgg tttttatcat aaattaaatt gaactgatat tctttatttt tcaaaatttc
    74281 ttcttgcaaa gaagattttc tttgttcatg aaatgggaaa ctaattttat ataattccat
    74341 agccttgtaa cataatttat tttcactggt tcttaaccgt ttaaatttca ctttactcct
    74401 cctaatcacg aaaattccaa tttatttatg agttcatttt accatagtca gtatttgcat
    74461 acaatgtgtt ttaagttgag tgtcatttct ttcttttatt tatgcttata tctcatagac
    74521 taagagataa ttgcttaaga cataactaaa aactattata ttaagctttg ataatctaaa
    74581 aacagtatat ttatggactt ttctaggtct aaaagtaatc ttaataattt tagaagttac
    74641 aaaaaagtta cataaatttg gaaagctata atatacatat ataatataag gttaaaactg
    74701 taatgaataa tattaattta aaatttgaac atggaaggag attattatgg agaaaagaga
    74761 aaagaggata ataatatcat ctttattaag tgtatcaatt ttaatgggtt tggtaagtat
    74821 atattctata ttaaataaag aagacataat tttaactgtt aagggtcaag agcaaaaagt
    74881 atcttccttt aagaaaacag ttgaggaact tttggatgaa cagggtataa agtataattc
    74941 tgaagataag attaatccaa gtttagatac agaactaaaa gatgatatga aaataaaagt
    75001 tgttaaggta actaaaagta aaaaagaaga gattgaaaaa attccatttg atacaaagca
    75061 tgtaaatgat agtaatttgt taaaaggaaa atctaaagtt tatcaagaag gtcaagaagg
    75121 agaaaaaaaa ctagtctata atttaactta ccatgatgga aagttagtca aaaaagtctt
    75181 atcaaaagaa gtaatatcta aggagccaac tacaaaaatt ataaaatatg gaactaaaga
    75241 aaaagtacta atagcatcaa gaggagcaaa tataagagga ggcaaacata tgaaagtggt
    75301 tgccactgca tatgcaggag atacaataac atctactggt acaactccaa gatggggtgt
    75361 tattgcagtt gacccacgtg taataccata tggaacaaaa gtatatatac ctaaactggg
    75421 tatgactttt gtagcagaag attgtggagg tgcaattaaa ggtaatagaa tagacatttt
    75481 tatgaacagt gaaggaaaag cttcaaattg gggaaggaaa agtatagata tatatctaca
    75541 ttaaattagt gaatagaata actttacttg aaggacaaaa aagtataaaa tatgaattaa
    75601 ctaaactgtt agattattct taaaacataa aaatatatct aaggggggat aagatgaaaa
    75661 tagtatgctt aggagacagt ttaacgtatg gatttggagt atcacgaagt aattcatgga
    75721 caaacattgt aaataaagag actcgattag aaatagtaaa taagggtata aatggtgata
    75781 caacaagtgg tatgttagct aggtttaatg aagatgttgt aaaaaattct cctgatatag
    75841 tttttataat gggtggcacc aatgatttta ttgctggtgc gggaaatgaa attattaatt
    75901 ctaatataat ggctatggtg catcagtcat attcaaaaaa tattatacct ataataggta
    75961 taccattaaa accagatatt ccaaatgtta gggaagattg gagttgtttt actgatttta
    76021 atatagtatc tcaaaactta gaatcatata gttattggat aaaaaaattt tgtatgacat
    76081 ttaatactaa tttcgtagat ttttattcag aatataataa aaatatggaa ctagaaggat
    76141 ataaaaaatt atattttgat gggttacatc caaccaaaga gggtcataga ataatggcag
    76201 acatttttat taattcaatt aataagtata tagaagagtt atagattaca ttaaaatgct
    76261 tgtttatgcg tcagttgcaa taaatacata cagtagagag gggtttatta tggattatac
    76321 taaaacttat gaagagtgga taaagggttc atattttgat gaagatacaa agctagagtt
    76381 ggagaatata aaaaataatg aaaaagaaat agaggataga ttctataagg atttagaatt
    76441 tgggactgcg ggtcttagag gaataattga agcaggaacg aatagaataa ataagtacac
    76501 agttagaaga gctacttttg gattggcaaa ctatatatta gaaaacacaa caaaagaaga
    76561 aacaagtaga ggagtagtaa tagctcatga taatagacac aagtctagac aattctgtat
    76621 agaatctgca aatactttgg cagcatgtgg tataaaagca tatatatttg atagtttgag
    76681 aactactcca gaattatctt ttgctgttag aagcttaaat gcaatcgctg gtatagtcat
    76741 aacagcaagc cataatcctc cagaatataa tggatataag gtatactggg aagatggagc
    76801 acaagttatg ccagagattg caaatgcaat aacagagaaa gtaaatagta tacatgatta
    76861 tagtacaata ccaaccttaa cggaagctaa taaaaatttg gtagttttgt tggacgaatc
    76921 acaagatact aaatttatag aagcagtgaa aagccaaatt ataagaaaag atttagttaa
    76981 aaatgtaggt aaaagcttta agatagttta tacacctctt tgtggaacag ggaatgttcc
    77041 aataagaaga gcattgaaag aagttggttt tgaaaatata attgtagttc cagaagaaga
    77101 aaatccagac ccaaattttg cagggcttga ttatccaaat ccagaagaga aaaaagcttt
    77161 aaatagaggt atattactcg caaaagaaaa aggagcagac cttgtaatag caactgaccc
    77221 agattgtgat agagtaggtg tagcagtaaa aactactaca ggtgagtatg ctttacttac
    77281 aggaaatcaa attggtggta tgctgacaca ttatataata gaaggattaa aagaaaataa
    77341 taaattaaaa gaaaatccta caataataaa aaccatagtt acatcagaat ttggagcaga
    77401 tatagctaaa gcaaataatg ttgatgtatt aaatgttctt acaggtttta aatttattgg
    77461 tgaaaaaata aaattatttg aacaaaataa aaatagaagt tatgtatttg gatatgaaga
    77521 aagttatggt tacttagttg ggactcatgc tagagataaa gatggtgtag tttcttcatt
    77581 gttaatatct gaaatggcag cattttacta ttcaaaaggt atgagtttat atgatggttt
    77641 aatagaactt tataaaaagt atggattttt caaagaacag actatatctt tgactttaaa
    77701 aggtatagaa ggtgtagaaa agataaaaga aataatatct tattttagag aaaatcaaat
    77761 tgattatata aatagtatta aagtagttga taaaaaagat tataaaaatg gtatagataa
    77821 tcttcctaaa tctgatgttt taaaatactt tttggaagat gaatcttggg tagctataag
    77881 accatctgga acagaaccaa aacttaaatt ttacatagct gtaaaaggag ctagtgatat
    77941 tgaagcagat aaaaagattc aaggtttgaa aaagtatata gatgacatgg tcgaaaaatt
    78001 aaaataaaat attattattt tataagataa ttttgtaata atatttattt agctgaaaat
    78061 tataatttac tttcattgag tagaatatgg aaatgttagt agaattgtta ttttcagctt
    78121 ttatttgtgt tatttttact taaataatta tattatagaa tgaatctgag tataattaat
    78181 attgtataat gaaattaatt tgatagaaaa ggggatattt atgtttggta tagataagaa
    78241 attgattgaa tatataattg caaaatccaa ttacagtgaa ttaagttcaa agtcagcaga
    78301 agtatctttc ttcttaatgt tgtccatatt tcctttttta atatttacaa taagttctat
    78361 agcttatata ccgattcttc atttaaataa gtatatagct ttgtttagaa atatgatgcc
    78421 agaaggtgct tttgctgtgt tatcttcaat catagtttct gcaatagaca atagaaacct
    78481 gaaattttta gcagtcagtt ttgtacttac tatgtggaca ttttcaagag cagtaaaagc
    78541 tcttattaaa ggaatgaata gagcctataa agttaaagaa actaggtctt tttttaaaat
    78601 tttatctata tcatttttat ttacaataat gcttttggtc cttatttttt tatctatgat
    78661 atttttagtt tacggtgaaa aaataggtta ttttattttt aatcttgtag gattggatga
    78721 gatatttata aaaatatggg atattctaag atacactgta ggtataataa caataatagt
    78781 gatatttaca cttttatata aatacactcc aaataaaaag ttaactatta aagaatctgc
    78841 gccaggtgct atttttgcta cttttgcttg gtttttagta tcatttttat attcttatta
    78901 tacaaattac tatgctaact atgaagtaat ctatggaagt atagctgaaa taattgtact
    78961 tatgacctgg atgtatttta gtagttggtc tatcgttatt ggctatgagg taaattcaag
    79021 attgtatttt agaaagataa gacatgaaat gttaaagtaa agtatttaaa ataaaagaga
    79081 gtatttagta ttataaatgt aattgaatta ataaagaaat attgacattt aagttaaaaa
    79141 gtctagaa
//
LOCUS       DAEMGG010000002        78224 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEMGG010000002 DAEMGG010000000
VERSION     DAEMGG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 78224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.002"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(94..1434)
                     /locus_tag="KPK24_000099"
     CDS             complement(94..1434)
                     /locus_tag="KPK24_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2297821.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCV
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
     gene            complement(2104..3195)
                     /locus_tag="KPK24_000100"
     CDS             complement(2104..3195)
                     /locus_tag="KPK24_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG2297822.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            complement(3963..5882)
                     /gene="thrS"
                     /locus_tag="KPK24_000101"
     CDS             complement(3963..5882)
                     /gene="thrS"
                     /locus_tag="KPK24_000101"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG2297823.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     misc_binding    complement(5946..6183)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6642..7244)
                     /locus_tag="KPK24_000102"
     CDS             complement(6642..7244)
                     /locus_tag="KPK24_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG2297824.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     gene            complement(7424..8242)
                     /locus_tag="KPK24_000103"
     CDS             complement(7424..8242)
                     /locus_tag="KPK24_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG2297825.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            complement(8245..9006)
                     /locus_tag="KPK24_000104"
     CDS             complement(8245..9006)
                     /locus_tag="KPK24_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297826.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            complement(9496..10608)
                     /locus_tag="KPK24_000105"
     CDS             complement(9496..10608)
                     /locus_tag="KPK24_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2297827.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATEYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            complement(10734..11738)
                     /locus_tag="KPK24_000106"
     CDS             complement(10734..11738)
                     /locus_tag="KPK24_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2297828.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            complement(12145..12993)
                     /locus_tag="KPK24_000107"
     CDS             complement(12145..12993)
                     /locus_tag="KPK24_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG2297829.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKKLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            complement(13029..13499)
                     /gene="trmL"
                     /locus_tag="KPK24_000108"
     CDS             complement(13029..13499)
                     /gene="trmL"
                     /locus_tag="KPK24_000108"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG2297830.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            complement(13693..14910)
                     /gene="nth"
                     /locus_tag="KPK24_000109"
     CDS             complement(13693..14910)
                     /gene="nth"
                     /locus_tag="KPK24_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG2297831.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKKEINKKGIDKKDIIEKKEINKKEMDKKDIIEKEEINK
                     KGMNKKDIIEKEEINEKEIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKG
                     ETEKKTKKSSSNKATQKTSKETKSKFKIKNESANEKDVNKILDELEKLYPDAKCELNY
                     GTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKS
                     KSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVF
                     RVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIK
                     EDCNYYKELNETK"
     gene            complement(15108..17345)
                     /locus_tag="KPK24_000110"
     CDS             complement(15108..17345)
                     /locus_tag="KPK24_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2297832.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTKQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            complement(17451..18365)
                     /gene="queG"
                     /locus_tag="KPK24_000111"
     CDS             complement(17451..18365)
                     /gene="queG"
                     /locus_tag="KPK24_000111"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG2297833.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKNRQQKGYLTG
                     MEEPIIESRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            19206..19610
                     /locus_tag="KPK24_000112"
     CDS             19206..19610
                     /locus_tag="KPK24_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG2297834.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(19867..20862)
                     /locus_tag="KPK24_000113"
     CDS             complement(19867..20862)
                     /locus_tag="KPK24_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG2297835.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(21146..21982)
                     /locus_tag="KPK24_000114"
     CDS             complement(21146..21982)
                     /locus_tag="KPK24_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG2297836.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKKELKMIKEILS
                     SK"
     gene            complement(22470..23033)
                     /locus_tag="KPK24_000115"
     CDS             complement(22470..23033)
                     /locus_tag="KPK24_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2297837.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(23131..24060)
                     /gene="glsA"
                     /locus_tag="KPK24_000116"
     CDS             complement(23131..24060)
                     /gene="glsA"
                     /locus_tag="KPK24_000116"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG2297838.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(24609..26276)
                     /locus_tag="KPK24_000117"
     CDS             complement(24609..26276)
                     /locus_tag="KPK24_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG2297839.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(26350..27105)
                     /locus_tag="KPK24_000118"
     CDS             complement(26350..27105)
                     /locus_tag="KPK24_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG2297840.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(27115..27651)
                     /gene="lepB"
                     /locus_tag="KPK24_000119"
     CDS             complement(27115..27651)
                     /gene="lepB"
                     /locus_tag="KPK24_000119"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG2297841.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(27817..28353)
                     /gene="lepB"
                     /locus_tag="KPK24_000120"
     CDS             complement(27817..28353)
                     /gene="lepB"
                     /locus_tag="KPK24_000120"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG2297842.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(28453..29763)
                     /locus_tag="KPK24_000121"
     CDS             complement(28453..29763)
                     /locus_tag="KPK24_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG2297843.1"
                     /translation="MTELPNEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(29842..30558)
                     /locus_tag="KPK24_000122"
     CDS             complement(29842..30558)
                     /locus_tag="KPK24_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG2297844.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            30803..32074
                     /gene="sleC"
                     /locus_tag="KPK24_000123"
     CDS             30803..32074
                     /gene="sleC"
                     /locus_tag="KPK24_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG2297845.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(32582..33238)
                     /locus_tag="KPK24_000124"
     CDS             complement(32582..33238)
                     /locus_tag="KPK24_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297846.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(34510..34920)
                     /locus_tag="KPK24_000125"
     CDS             complement(34510..34920)
                     /locus_tag="KPK24_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297847.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(35136..36518)
                     /locus_tag="KPK24_000126"
     CDS             complement(35136..36518)
                     /locus_tag="KPK24_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2297848.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNGKDVLYTDSGEVSIEQSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(36529..36888)
                     /locus_tag="KPK24_000127"
     CDS             complement(36529..36888)
                     /locus_tag="KPK24_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2297849.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(37093..37872)
                     /locus_tag="KPK24_000128"
     CDS             complement(37093..37872)
                     /locus_tag="KPK24_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG2297850.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTVISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
     gene            complement(38333..38944)
                     /locus_tag="KPK24_000129"
     CDS             complement(38333..38944)
                     /locus_tag="KPK24_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2297851.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(39036..40025)
                     /locus_tag="KPK24_000130"
     CDS             complement(39036..40025)
                     /locus_tag="KPK24_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG2297852.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYIEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAINNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(40092..40955)
                     /locus_tag="KPK24_000131"
     CDS             complement(40092..40955)
                     /locus_tag="KPK24_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG2297853.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(41061..41657)
                     /locus_tag="KPK24_000132"
     CDS             complement(41061..41657)
                     /locus_tag="KPK24_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG2297854.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(41697..42500)
                     /locus_tag="KPK24_000133"
     CDS             complement(41697..42500)
                     /locus_tag="KPK24_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG2297855.1"
                     /translation="MIKLTDEEFIVLVNYVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(42536..42976)
                     /locus_tag="KPK24_000134"
     CDS             complement(42536..42976)
                     /locus_tag="KPK24_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG2297856.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(42989..45091)
                     /locus_tag="KPK24_000135"
     CDS             complement(42989..45091)
                     /locus_tag="KPK24_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG2297857.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(45352..47055)
                     /locus_tag="KPK24_000136"
     CDS             complement(45352..47055)
                     /locus_tag="KPK24_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG2297858.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(47081..48040)
                     /locus_tag="KPK24_000137"
     CDS             complement(47081..48040)
                     /locus_tag="KPK24_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2297859.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(48176..48694)
                     /locus_tag="KPK24_000138"
     CDS             complement(48176..48694)
                     /locus_tag="KPK24_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG2297860.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFRVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(48737..49222)
                     /locus_tag="KPK24_000139"
     CDS             complement(48737..49222)
                     /locus_tag="KPK24_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG2297861.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(49219..49836)
                     /locus_tag="KPK24_000140"
     CDS             complement(49219..49836)
                     /locus_tag="KPK24_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG2297862.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(49901..50263)
                     /locus_tag="KPK24_000141"
     CDS             complement(49901..50263)
                     /locus_tag="KPK24_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG2297863.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            complement(51007..51504)
                     /locus_tag="KPK24_000142"
     CDS             complement(51007..51504)
                     /locus_tag="KPK24_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG2297864.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            complement(51679..52590)
                     /locus_tag="KPK24_000143"
     CDS             complement(51679..52590)
                     /locus_tag="KPK24_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG2297865.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            53515..54366
                     /locus_tag="KPK24_000144"
     CDS             53515..54366
                     /locus_tag="KPK24_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG2297866.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            54370..54840
                     /locus_tag="KPK24_000145"
     CDS             54370..54840
                     /locus_tag="KPK24_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297867.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            54853..56163
                     /locus_tag="KPK24_000146"
     CDS             54853..56163
                     /locus_tag="KPK24_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2297868.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            56375..57121
                     /locus_tag="KPK24_000147"
     CDS             56375..57121
                     /locus_tag="KPK24_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2297869.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            57483..59024
                     /locus_tag="KPK24_000148"
     CDS             57483..59024
                     /locus_tag="KPK24_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG2297870.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            59059..60165
                     /locus_tag="KPK24_000149"
     CDS             59059..60165
                     /locus_tag="KPK24_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2297871.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(60496..61782)
                     /locus_tag="KPK24_000150"
     CDS             complement(60496..61782)
                     /locus_tag="KPK24_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2297872.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(61905..65003)
                     /locus_tag="KPK24_000151"
     CDS             complement(61905..65003)
                     /locus_tag="KPK24_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2297873.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSSDFEKLLY
                     QK"
     gene            65728..65880
                     /locus_tag="KPK24_000152"
     CDS             65728..65880
                     /locus_tag="KPK24_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297874.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(66173..66877)
                     /locus_tag="KPK24_000153"
     CDS             complement(66173..66877)
                     /locus_tag="KPK24_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2297875.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(66868..68475)
                     /locus_tag="KPK24_000154"
     CDS             complement(66868..68475)
                     /locus_tag="KPK24_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG2297876.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(68729..69907)
                     /locus_tag="KPK24_000155"
     CDS             complement(68729..69907)
                     /locus_tag="KPK24_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297877.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(70133..70627)
                     /locus_tag="KPK24_000156"
     CDS             complement(70133..70627)
                     /locus_tag="KPK24_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2297878.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(70628..71389)
                     /locus_tag="KPK24_000157"
     CDS             complement(70628..71389)
                     /locus_tag="KPK24_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2297879.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            72111..74945
                     /locus_tag="KPK24_000158"
     CDS             72111..74945
                     /locus_tag="KPK24_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2297880.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            74988..76232
                     /locus_tag="KPK24_000159"
     CDS             74988..76232
                     /locus_tag="KPK24_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2297881.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(76647..78224)
                     /locus_tag="KPK24_000160"
                     /pseudo
     CDS             complement(76647..78224)
                     /locus_tag="KPK24_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT        25950 a        13577 c         8143 g        30554 t
ORIGIN      
        1 tatacattaa ttcttatata ttaaacttct atgcatcact tttatacatt aattcttata
       61 tattaaattt ctacacatca aatttatata catttaaatc acatgattaa ctgctttctt
      121 cacatttcta agtggaatag caattttaac tacaaatttt ttatcatcat attcaattac
      181 aacattacca ccatacttat ttacagagtt ttttacactt tttattccaa gtccatgaag
      241 agaacctttt ttgtctgtca aaatatctcc atttttagta ataatcttat tcatcttagt
      301 attttcaact cttattaaat aaaaattttg cataacatca cctttaacac aaataactct
      361 atttccacta tcaattttat tacatgcctc aattgcattg tctaaaatat ttgaaaatat
      421 atgacatata tccactaatt caacagcttc acaacaacta aaatttattc ctatttttag
      481 ttctatatca ttcttatcac aaacctcttt cttttcactt aatatagcat ctaatataaa
      541 attaccagta ttaaataaca agtcattact ctgaatttct acctgcaaat tatttaaata
      601 agaagtaaca tcaatacctt ttttaatcat agcatttata cacaagatgt gatttttcat
      661 atcatgactt agtaatctag tttttaaata tttcttttct atatcttcat aatactcatt
      721 ttcatacttc aaagtatttt taatcatatt attttctaca actaaagtat tatattgctg
      781 taatttttta gcaattaaaa ctaaagtgat acttgatata agcaatacaa cagaaatcat
      841 aacgaattgg aaattatgta ttaagctacc agaccctgga ttaaatatgt attcaaaaat
      901 tacaaaaaaa gatgctatat tagcgactat aggaataact atataaaata atgtcctttt
      961 atttatttct atattaagtt ttatctttaa ggctctatat aaaagcaaag acacaatcaa
     1021 aagtatcttt cccaatatta tagactcaat tctgtatata tctcccgtcc ccatactact
     1081 catgtcattt atagaattaa gccaaactgt taaagatata ctcaaagcat ctgtggcgat
     1141 tagtaacatc caataaatta aggatattaa tacagacttt ataaacccca catcatatga
     1201 gattttataa aatgcaatag taattatcat agatataatt actcttatat ttggaggtac
     1261 atcaaaaaaa tatattatgt cagcaaaaac aataatacct accacacaaa agtttatttt
     1321 tttgttagaa atccttttct cacttgtata atctagcatt ttcttacaaa gaaaccattc
     1381 tatcataaag gatatcgcaa tgaaaatatt ccaaaataag cttgacttta tcataatact
     1441 ttctcctaac gtattactaa attatttttt tatattttat gtataattta attatatacg
     1501 actctacacg tgtagtaaat agattgtcaa aattattcca aataagtcca tatttctttt
     1561 cttttttaat tatatcacta aatttgctaa attgaattaa actttttatt gcttttttta
     1621 ataataacta tataaattta tctataacta ggtataaaat atattattta taaagttttt
     1681 acattatttc cataataaaa tcttataaat attacaataa aatatttttt tccatgatat
     1741 accttgataa aatctctgct tctttaagca gtaactttga gttgaatcat tatagtgatt
     1801 tataacattt atagaaatgc tgttatagat ttatatttaa acactacaaa tggaaattta
     1861 actattataa aaaacaatca ttagataaaa tgattcttac aacaaaagca tttctattgt
     1921 agatttatta gtaaataata aatctacaat agaaaaaact agaagtacag tatcataaaa
     1981 atataattaa ctgtacttct aaattattgt ttttaaaata aattttcatt cattaaaaaa
     2041 tacacataaa tatttccaga ctccgattca gcaaacttat ataagttcac tttttattta
     2101 tcatcaatta ttttcatata cttcttttat ctcatcttca cttaagtcaa aatcattaaa
     2161 tttatttaat atgtcatcta atttaaaacc attttttttc aacattataa ttattttata
     2221 atctttttct tttgcaactc taattgctgt ctctctttca atctcttctt taaaatttct
     2281 acttttattc atgatataag cacctctttt cagatttaat aaatattctt cattgctacc
     2341 taattctttt aatctatcaa gtgctttttt aataatagaa tcattcttag ctataactct
     2401 tacatctaag ttagaaggat tactaataag ttttccccat agcataattc tatttactat
     2461 atagtcagat ttatgaattt ttggaagctc tataaaatat aatgcagttt tactagaata
     2521 aatatctgga tgcccgtcca ttgttaatct caggcaattt acataatctt ctatgtgatt
     2581 atattttaca tgatttagta tatgtattcc aatagttttt ccaatttctt tatactcttt
     2641 accttcttct aactttctac catgtacacg actaagataa tattcagctc tttccaaata
     2701 atcgttctca aaagctcttt gcatctcaat atttatatga ttattttctt catcttcagc
     2761 caaaatatca agtttaggtc ttttctctcc aatgtaatta attgttggat cagtttcaac
     2821 tctaattatt ctctttattt gagtgaacgg tttgatattc ctgttagcat atggagcatc
     2881 ttttagaata tcatttataa aatttacaag taaatcatat tcattattta aagtgaacaa
     2941 tgatttaaat acctcatcat actcgggctt agcatatcca tcttcagtca caacttctcc
     3001 aacattttca ttagtcaaaa tctcttttgg aattaacgcc ttgattttat ctaattcatc
     3061 attagaaaaa ctacttctac taagtatatc atttaatttt ttggtccatt ctttatcttt
     3121 ttcttcaatt atagttcgct tatattcttt tgatgtttta ataatcttag atttttcttc
     3181 tactttttct atcatttcaa cacctctctt tttcctcttt tgtatttatc atttcttgaa
     3241 attaaactaa gtcactacct cttttaaaag ttcttgctcc ataaacactt tcttaaactc
     3301 taatgtctgt gtatgtactt gacattctct ctctatctaa agttaaataa attaatctat
     3361 gtatagaggg taagtccaaa aataacataa aacactaatt aatcatttat aaagcattaa
     3421 ttaatcactg acaatgctgt tattgtcacc acatagcacc aacatagatt cttctattat
     3481 agggcaatta tctatgaaaa atagactcat aagattagaa aatgtactta aattttagtt
     3541 catttaaata ttcattttga agaaagaaat ttaactttag actaaaaagg aaatatttca
     3601 agaggattaa gaaattttct taaagggata ataattttta tgaaaatgga acaaatatga
     3661 aaataaagtt atgattttta cagccataac cttaccaaca agcacttaat tatcactcaa
     3721 aaaataaacg tttatttaaa gaatgcacat aaatatttcc atgttcaaaa cacttctatg
     3781 atatatttat taataaatct accatacaaa aaactagagg tacattaatc ataaaaatat
     3841 gattaactgt acctctaatt atactcatct tttcataaat ttaatcacta tgcatcaaca
     3901 tatcaacata ttgattaaac ttatcttata taacaatatc ttagctacta atataatata
     3961 tattagtctt gaataatact ttctcttgat tcaatttctt ttgaaatact agctataaat
     4021 tcatctaatt taatactacc tatttcacct ttatctcttg atctaacaga aacattatta
     4081 tcagctactt ctttttcacc tactattagc atgtatggca ctttttcaag ctgagcttct
     4141 cttatcttaa atccaatttt ttcagctcta tcatctaatt caactcttat acccttatca
     4201 aataactctt tcttaacttc atttgcataa tccataaatt tatcagatat aggaagtatc
     4261 ttaacttgag ttggagataa ccaaactggg aatttaccag cataatgctc tattaatata
     4321 cctataaatc tttctatact accaaaagca accctatgaa tcataactgg tctatgcttt
     4381 tcaccatctt gaccaatata agtattatca aattgtcttg gaagctgcat atctaattgg
     4441 atagtaccac attgccaagt tcttcctaaa caatctttta gatggaagtc tatcttagga
     4501 ccatagaatg ctccatcacc ctcattaata gtatatggta gacctaactc ttctaaagct
     4561 tctcttaatc cattttctgc tgcttcccac tcttcgtcgc ttcccataga gttctctggt
     4621 cttgtagata attctaaatt atattcaaat ccaaaagttt tgtatatacc atctattaag
     4681 ttagctactc cttttatttc gtccttaatt tgctctggta acatgaatat atgtgaatcg
     4741 tcttgagtaa atgctctaac tctcattaat ccttgtaatg caccagataa ctcatgtcta
     4801 tgaactctac caagctctgc aactctcatt gggaaatctc tatatgaatg tagttgagag
     4861 ttataataaa ttaatccacc aggacagttc ataggtttta tagcataatc ctcatcatct
     4921 attttaactg tatacatatt ttctttgtaa tgataccagt gtcctgaagt ctcccataat
     4981 tttctattta atatgattgg agattctatc tcaatatatc cagcttttct gtgtatttct
     5041 ctccagaatt ttaataattc atttttaagt tccatacctt ttggtaaaaa catagggaat
     5101 cctggacctt cttcaggtat cataaataat cctaattctt tacctaattt tctgtggtct
     5161 ctctttttag cttcttctag taaatgtaag tactcttcta agtctttgtt tttctcaaaa
     5221 gaagttccat atattctttg aagcatctta ttattttcgt caccacgcca atatgcacct
     5281 gctacacttt gtaatttaac agcttttacc tttttagtag atggtaaatg tggtcctcta
     5341 cataaatcag taaaatcacc ttgcttgtag aaagatatta tttcactttc aggtaaatca
     5401 gatataagtt ctaccttata gtcttctcct tgttctttca taagctctaa agcttcattt
     5461 ctaggaagtt caaatctttc tatttttaaa tcttctttag ctattttttt catttcagct
     5521 tctattttct ctaggtcctc tggaactaat ctatggtcta aatctatatc atagtagaat
     5581 ccattttcta tacttggtcc tatagcaagc tttgcttctg ggtaaagtct ttttatagct
     5641 tgagctaata tatgtgctga tgtatgtcta aaaacttcct taccttcttt atcttcaaac
     5701 ttaaataaat tcaagtcaca atcagtatct ataatgtggt ccagacctac aacctctccg
     5761 ttcacagacg ctgctaccac atttctagct aatccttcac taatggattt agctacatcc
     5821 ataacagaaa tagcattttc aaattctttt attgagccat cttttaaagc aactttaatc
     5881 attgtttttt cctccttatt gaaataattt ttagattttt tgtaataaaa aatcgcccca
     5941 aatatgtttg ggacgatatt aattcgcggt tccacccaag ttggcgtata caagtatata
     6001 caaccctctt gatgactata aggtcgtcac ccgagcactt tcacgtatag tacaaaacaa
     6061 atactgctaa taagctcagc tccaaggtag ttttcaaata gacctattac atgggctctc
     6121 agccatagac ccaaattctc tgagaatggt ttctactcta ctcttcctct tcaacactat
     6181 gatttttaat atattttcat tataacagat agttatacat caatcaacat taatgtaata
     6241 tattaattac ccattatttt tcatctcaat cagctgtaaa ccaatattct tatattttta
     6301 tattaaatta atctcttgat ttatagtgat attataatat tttcataaat ttaaaatttt
     6361 taaaacgtat ccacatgcat gatagatatg actagataaa tactaaaact aatttttatt
     6421 aaaactaact tctaattttt gtactctttt taggttttgt atacttaaat acaatcaata
     6481 cttaatatag ttaataagta gtacagtaaa tacttaaata ctattaataa gtaacacaat
     6541 caaataaata aatacaatta atatctaaat gctattaata agtaattcac tcaaataggt
     6601 aaatataatt agtacttaaa taaatgctac ttctcatata tttaaataaa catagtaatt
     6661 aacccttgag ctatacctat taaaaaccct aagacaagcc ccaaaaactc tatatgtttt
     6721 aattctttct tagctattct tattattatc tcttccaatt catataaatc tagttcatta
     6781 atcttatctt cgaccatttt ttgtatatct atacgttcat ttgctttatt tattatattt
     6841 ttactcaatt catctatact ttgcttaact tcagactcaa ttatctctcc tagataatct
     6901 tgtatcatgt tttttatacc actcgggata aatgaaactt tctcatgtat tataattttt
     6961 acccttgctt ttatgtatct tacaacttct tccttatctt cgtcagtaat gatatttgca
     7021 agtatttcgt ccatagaaag aaattcttct tgaattattt ctcctacatt tgctgctatt
     7081 tctgctcttc tctttggtat aagacctact atttctatat ttaaaatagg tattttaata
     7141 ggctctattg gtctaaatat cagtcttata gcaactacat tcgtaacata acctataaat
     7201 ccaccaataa cagctaatat cagtatccta attaaattat ccattataaa attactcctt
     7261 atcaattgta tatttgccag ctatattgta ttaatcaagt agatattctt tatacttaat
     7321 aacactagtt atactatagc tttatataca attataagtc ttttgtgtcg atttaaaaag
     7381 aattttttta attttatatg gatagctttt atatgcttag attctagttt tccataatta
     7441 tactaacttt attttcaaaa attgcttcta cagtatctat aatttccttt gagcaattat
     7501 tatttaacaa atccaatatt tctatttttt tgggacatag agttaataag gtacttatta
     7561 aaaagtcctc ataatttaaa ttttctctta ttaccatatt tataatttct tcatcatcta
     7621 tactgtctat cttatttcca tttttatcgt ataaaacaaa tgtattatct tccattatat
     7681 gaactttaag caaatcaatc ttttcttctt gagtatctat aaagtatttt aaaactctta
     7741 taaattcatc ttggtctttc tttattaaat actcctcaac tgcaatatca cctatagcag
     7801 aaatatattt cataaattct ctcattctaa atctaataaa accttctaca tttataaaat
     7861 cattttcttc tatataattt ctcaccctag taaatactgt ttctctcata aaaatctctt
     7921 ttttagcaaa tatgttcagt gaatatatgt atatattatt tttatcttca ggatatgtac
     7981 gcccatagca ttttagaatg tattctttca aaagtttctc tttcattata cttattatca
     8041 tactagttat ttccgttgat atctcatcaa tatttaaagc tctttgttca gctttagtac
     8101 cattaagtat ttttttattt aacacttctt gctttgcaga atctatcact acttctacaa
     8161 tgtcattcgt atatattaat ctatcgttta cattattatt cctcaaaaat ttcatggaag
     8221 aaatattatc cttctcaacc aaagttatcg ctcctttctt gtcatgagag gaactataga
     8281 acctatatct agtaaatttt cacagctaag tgtaactatc tccttgtttt tactttctac
     8341 aaaacttttt atacgttcat agtcaggatg ttttttaaca tctccacaaa aagctagttt
     8401 atccttagaa atcaaccctg tacatccacc tataaatcca taattcatct caaataaatt
     8461 tatgtgacct ttttctatca ataaacactc tatttctgta ttcttcatac ttttccaaat
     8521 acctttatct gatgttatta tagacgttct atctactata cagattgagc atttagaata
     8581 gccctgctct atatttatct ttttaagttt attattttca atataatcta atattgcttt
     8641 gtcagtatac ttaaaattgt gtatagcata gtcaccaact atcgcaatat tgtaaggtat
     8701 atcataaggg tatctattta ataaattagc ttctccctta attatattaa atttatatct
     8761 acttaataac tctttgtatt gattatatac attcggcgca acaattatat ctccttttcc
     8821 taaattacaa atacatatat ccgggtgata ttttatgctt tcatataatt ctttacattc
     8881 tattgttttt attatctcta tatttcttaa ttttaattca ttttccatta gataactaat
     8941 tcttttgtcc accaaagcta gacacaaatc gtcatctagt ataaatggtt ctcttgtaaa
     9001 ttccattttt tactcctttt tctttttttt gataaatttt ttatgtgaat ataaataaag
     9061 tgtgtagcag aatttttctg cccacacact tattgttata attttatata gttaatctaa
     9121 gcttaattag tatagctttg tacagttggt tcaaatttaa taaatatatc tttttatcac
     9181 taatctaaac ttaagcagta tattccgtac agttagcaca aatttaataa atatatcttt
     9241 ttattgctaa tctaaactca atcaatatat tttgtacagt tagactaaat ttaataaata
     9301 tatcctttta tcaccaatct aaacttaagc agtatattcc atactgttaa cacaaattta
     9361 ataaatatat ctttttatca ctaatctaaa cttaatcaat atattttgta cagttagcac
     9421 aaatttaata aatatatcct tttatcacca atctaaactt aagcagtata tttttataat
     9481 cttctacatc aataattaat atgtatcttt cactttgtta tactcttttt ttatgtattc
     9541 caaaacatct ggtggtatat ttctactgct ctttgaagtc aagccaagtt ctccatcttt
     9601 tattttaaat tccattactt tacttccctt aaaaccatct tttgcaacgt tctttatcag
     9661 ttcatataca ggttgttcta catcttttat aattgaactt attacattat ctggagccat
     9721 atcgtattgg tctttattaa tagctataac tttacctcca ttttctcttg ctacctgaat
     9781 agctctttta ctatcatcct ctgtagctga aaatacaaca tctctatcag aatctctcat
     9841 ttcctttgca atagttctag ctgattcagg gttttcaaat atatttgaat acttaactag
     9901 taactcagca tctttatttg aagttttaag cccctttcta aatccagatt catacttatc
     9961 tctactagaa ccctcaactc ctcctataaa accaaccttc ccagtatcag tcattttacc
    10021 tgcaataagt cctgctaaat atccagtaac attatcttga aatgatacag aaattacatt
    10081 agctggctga actttataag tatagtcaac tattgcaaat tgctgtttgg gatattcagt
    10141 agcagcttct ttaattggct ctgcaaatct ggctccaact gctattatca aatctggttc
    10201 atacttatgc agtttttcta tactctcttt aaaactttta gaagtctttg gtttttcaag
    10261 ttttatccta acatcaaaat cttcgtcagc cctttttaag gctgatagta cattgttatt
    10321 atatgattcg ttatttaagt tttcggtatc taacaatact ccaatagttt ttcttacata
    10381 tgaatcatct tctgtgtcat cttctttatt cttactttta ctatttgatt tatccttttc
    10441 tgctgaagtt ggcaaacttt tagactcact ctgtttcgat ttagcactat ctatattaga
    10501 attattctta tttactttat caaacccaaa atcaaaatta catatagcta atataaaaat
    10561 tgtaaaaact actacaaagc aagccatcaa tatccttttt attttcacaa aattcactcc
    10621 ctgatttaga taggtctgac ctacctctgc atatgtcttt ctatatatgt ctattataac
    10681 atccaattca cctcataact aaagtcataa gtattcttgg atattatagt agattattta
    10741 ttgttcttat taacatattc taacacatct ggaggaatat tttgagaact attttgggca
    10801 agcccagtac ttccagactt aactgtgttt tctataactt ttccgccttt ataatttcct
    10861 tttacaaaac tttgtatcag attataagta ggattctcaa aatcttttac aatagaactt
    10921 ataacatttt ctggagctaa ctcatgttgg tctttattcg ttgctataac ttttttattt
    10981 tttgccctta ctgcatctat tacagctttt gaatcatctt cagtagtaga aaaaataata
    11041 tccactccat tatccatcat ttttttagct atagattcta ctgatttgct atctttaaaa
    11101 acatctgcat actcaactga aatatccttt atactactat tagaaaattt aacaccctct
    11161 ctaaaacctg attcaaattt atctctacta gaactctttg ttcctcctat aaaaccaacc
    11221 ttatcgcttt cagtcatttt tccagcaatt aatccagcta gatatccaga tttattatct
    11281 tcataagaaa ttgaagttat attagaaggt tgcttgtcat actcatagtc tataattgca
    11341 aattgttgct ttggatattt cttggctacc atctccaatg gttttgcaaa tctagctcct
    11401 acagctataa ttaaatcgta attgtcatta cataaaatat ctatactatt agaaaaagtt
    11461 gaggaatcat ctgattccac tttgtttgta tctatattaa gtttcttttg agcattgttt
    11521 aatgctaata aagctgaatt attcatagct tcattatttg ttccttcaat atctagtatc
    11581 attgatacag atttttttga attactttta tcgcttatat cacttttatt atctttgcta
    11641 gagctaggat tattgcttgg gttattagca cacccaacca acattccagc tatcattgct
    11701 acagttaaca tcaatatcaa tattcttttg aaacgcatta aatttcactc ctcatacatt
    11761 tacgatattt ttatatccta tttactttta catcctattt acttttacat cctatttact
    11821 tttacatcct atttactttt acatcctatt tacttttaca tcctatttac ttttacatcc
    11881 tatttacttt tacatcctat ttacttttac atcctattta taattttttt atatacaatt
    11941 tacaagtaca ttttgaaatt tttttaataa atcttactat aaaaaagtgt gcaatagatt
    12001 cttatctatt tacacacttc cctacaaact aaatctaata tatcaacact taatttcatc
    12061 caaaataaat ccaatttaac atctcaataa ttaatattct atcctaattt aataaaaaaa
    12121 taaataatgt catccaaaaa tatattattt attttttaag catatgatac cagtaacacc
    12181 aggaccagca tgtgcaccta tacacacacc aatttggaaa aatctaattt ctttaggtct
    12241 taattcatct tgcataacct tagataattg ttctttttcc ttaaaatcat cactatatcc
    12301 tatatataca atttggtctg ataaatcatc accacaattt gattttatca attccatcat
    12361 cttagaaatt acattcttct ttcctcttac ttgagatatt tgcgaaacta atccatctct
    12421 tatttcaaga atcggtttta tatttaatat acttcctatt gctgccttag tagatgatat
    12481 tctacctccc ttttgtaaat attccaatgt atctactgaa aatataacaa aagccttctc
    12541 ttttaattca tccaaaacag gcaatatttc agcaacagac tttccttcac ttgcaagttc
    12601 tcctgctttt attacaagta aaccagttcc catacaaagc aagttagagt catatgtata
    12661 tattttacca tctacatcat tctttgccat tacagcactt tgatacgtac ctgtagctgt
    12721 ggcagaccct gctatataaa gtatctctct atcctcattt gtatatttct caaaaatttc
    12781 cttaaattgg gcataagtag cttgagaagt ttttggatat acattttctt ctctaagctt
    12841 tttataaaat tcatctccag atatatcaat tctatccttg tattctcttt cctccagtat
    12901 aactgttaat gaaactatat ctatatcata tttatctaac aaatcttttg gcacgtccga
    12961 taaactgtca cagattatct ttagattttt cattaagtat gctcctttct catttaggct
    13021 tgttgaaact acctcatatt tgggtatcct atttgtctta aagcctcgta cactacaata
    13081 gcaactgaat ttgataaatt taatgaccta gctttttcaa catccaacat aggcactcta
    13141 atacaagtat ccatatttgc tttcaacaaa ggttctggta atccctttgt ttctttccca
    13201 aatactataa aacaattttc ttcatatttc acatctgaat gtgactgttt agcctttgtt
    13261 gtagaaaaaa agtatttact atttggatac ttttcttgta attcttcaaa gctatcgtaa
    13321 tattgtatat cagctatatc ccaatagtca agcccacatc tttttaggtg tttatcatcc
    13381 aatgaaaaac ccagtggttt tactaaatgt aaagttgaac ctgttgctgc acaggttctt
    13441 attatatttc ctgtattttg aggtatttct ggttctacta atactatatt taatgacatt
    13501 ttaatcctcc ctaagtatcg cttttattta tcaaatttta ttttataaac agaataacaa
    13561 taatattata ctattatata aaataatgtt aataataatt tttatttcat gtatataata
    13621 gttttttatc taacattttc acaagtatta ttatatcaga aaatccctct tgtacaactt
    13681 actttctaaa agttatttcg tttcattaag ttctttataa taattacaat cttcttttat
    13741 tgggcaactt gcacattctg gatttctagc tttacacatt cttcttccat gaaaaattaa
    13801 tacatgatgt gagtgtgacc atctttcttt tggtatagct tccataagtg caaattctgt
    13861 tttttgagga tttggctcgt ccactattcc tattctattg ctaactctaa atacatgagt
    13921 atctacagct attgctggat gattaaaagc gttactaagt acaacccctg ccgtttttct
    13981 tccaacacca ggtaatttta taagcttttc taagctatca ggtacttcac catcatataa
    14041 ttcacatagc tgttcagatg tatcctttat tttttgagac ttgcttttgt aaagaccaca
    14101 actctttatc tctttactaa tttcttcaat gctcaaatta gcaaagtctc tagctgtgtt
    14161 gtacttttta aacaactctg aagtaacttt attcactctt acatcagtac attgtgctga
    14221 taaaatagtt gctattaaaa gttcaaaggc tgtcccataa tttaactcac atttagcatc
    14281 tgggtacagt ttttctaact cgtctaaaat cttatttacg tctttttcat ttgctgactc
    14341 atttttaatt ttaaacttac ttttagtttc tttacttgtt ttttgtgtag ccttattaga
    14401 actactcttc tttgtttttt tctccgtttc acctttttta gtactttttt tacttacttt
    14461 gatacttttt ttatcttctt caatatccat aaaattctct atatctttct catcaattat
    14521 ctcatctttt ttaccaatca ttttatctat ttctttttca tttatctctt ccttctcaat
    14581 tatatctttc ttattcattc cttttttatt tatctcttcc ttctcaatta tatctttctt
    14641 atccatttct tttttattta tctctttctt ctcaattata tctttcttat ctattccttt
    14701 tttatttatc tctttcttct caattatatc tttcttatct attccttttt tatttatctc
    14761 ttccttctca attatatctt tcttatctat tcctttttta tttatctctt ccttctcaat
    14821 tatatttttc ttatccattc cttttttatt tatctcttct tccaaaatta catctttttc
    14881 atttatctct tttagattta catttttcat aattatcccc cttcaaaata ccaatttata
    14941 aaacgctatt tataaatagt ttaatcatat caacaaaaaa ataaacatca atatataaaa
    15001 catacaaaat aagaatttaa tttatatata tttacaaaaa aagcaaaaac ctattaaagt
    15061 tcttgctttt tattatacat aaatacccat taaattctat aataaactta ttcttttgac
    15121 acagtttgta tcttgctgaa agatattaat ttttctttta ccaatttctt tatttcttca
    15181 aatttaacat ctgtcaatat ctctatagcc tcttctactc tttcaacagt atatatctta
    15241 aattttccat ctcgcactgc atcattaact tcatctgata aaacgagatt tctttgattt
    15301 tttctaggaa ttatgacgcc ttgctcacca tttaaccctt tagttttaca tatagaataa
    15361 aatccttcta tcttttcact tataccacca actacttgta tatcaccctt ttgatttata
    15421 gaacctgtaa cagcaatatt ttgtttaatt gatactccac taagagatga taatagtaca
    15481 tataattctg cagaagatgc actatctcca tctattccac cataattttg ttcaaaacaa
    15541 acatatgcat ttagtgacaa tagtaaatcc tgtgcaaaat tctcagaaag atagccacct
    15601 aatataagta ctcctttgtt atgtatagag ccactcatat ttacttctct ttctatattt
    15661 actatgcctt tacttcctgg gctagtggtc gctgtaatcc ttgaagctct accaaaagag
    15721 tattctcctg tacttaagac tgacaaacca tttatcactc ctacttttct tccctctgtt
    15781 tctattagag taaatccatt ttcaattgat tcatccatat gtgtttccac tctattgatt
    15841 ctttttctct tttcacttat tgctttttta acatcacact gctttgtata ctccgaattt
    15901 ctaaactctg cataagcact tccttctatt acaacttcca ttattttatt aaacttagtt
    15961 gataattttt ctaaatcacc agataatcta gtgctaaatt taataacctc ttcaactgct
    16021 gatttagtaa aatgccttaa attattctta tcacattggt atgctataaa tcttgctatt
    16081 ccttcttcat tatcttcatt tttatccatt tcactatcaa agtctacaaa tactttaaaa
    16141 tatttttcaa aatcttcatc atatctatat aaaatatcat atatatagtg gcttccaatc
    16201 agtactaatt taacatccaa tggcatattt tctggtttaa ttgctgtttg tgtttccaat
    16261 attacttttt tagtctgcaa agtcttttta agaatttccc aagacattgt atattttagt
    16321 aattggtctg cataaagtac taaataacca ccattagccc tatgaaatgc tcctgataat
    16381 agttttgtga aatcagtctt tatatttcca tttgcataat catactctgc ctttccaaat
    16441 agatttgctg gacttgggtt tatctctact acaacaggtg ctgattcttt attctttgag
    16501 cttccattgt ctacaaatag gttaatttta tatttaataa aatgctcttt atcatactta
    16561 tcttttaact cctcttcatc taaatagaat aaatacatat attctattat atctttcttt
    16621 atattttcca gataagtttg aatcttatca taattttcat atttttcaca caatctcttg
    16681 atatgaggtt ctactacaag tttagctatc tcttcttcaa gctctaacac tgctttttca
    16741 gctatttctt ctaagtttct aattttatat acgacctgta tcgccatatt ttctagttct
    16801 cttttagctt tgtaaaactc ttcatcagac tcatcacttt catcttcctc ttctttcaaa
    16861 gggataaaaa ctattcccat tttactctgt tttaatttaa atcctttttc ttctccatat
    16921 tttttaattt gcttcaaaag catatctttt tctatttcat attcatccag aagtttattt
    16981 ttttctattt caaaattttc actttcaaac gcatttttaa aatcttctaa aagactttca
    17041 attaatttct ccatatcttt cttaagttct cttccaaacc ccttttctaa ttcaactata
    17101 ataggctctc tagggttttc aaaattatat atataacacc aatctttatg cgtatttttt
    17161 ttggatgcat actcatttaa aacctttaag gcataagtac ttttaccagt tccaggttca
    17221 ccagctatat atatgttgta cgctggatta tttattttca accctaattc catagcttct
    17281 acagctcttt cttgacccaa tatataatct agtggattta tttcagatgt attattatat
    17341 ttcaatattc ccccacctcc ccatatttaa ctttaatgat ataatatgac ttttacacaa
    17401 caagtgttaa agtaaataca cattaaagta tatgaaacag actcttatta ttagtcatca
    17461 tacttatcta aaattatttc catatttctc tttattatat ttctacctct ccagctaaaa
    17521 gctctatctc catatctcct tttaaattct ttatttgaaa tattttgtat ctcatcataa
    17581 tccaaataat ctattatatt ttctttaaat tccaaaatat gagttgttgg tatttcaaca
    17641 ttatgtggac atacttcttg acaaatatcg catccaaata cctttttgcc attctttaaa
    17701 acaacttttt ctttctctga caaatctcct tttttttgag ttatatatga taaacatttt
    17761 ttggggttca tatcataatt tcccaatata gcatttcccg gacaatattt tacacactta
    17821 ccacatttaa aacaactttt ttctagtggt ttatcttctt caaatttata attatttact
    17881 atatacccta taaatacgta agaaccatat tcatcagtta tgatattatt atttattcca
    17941 aaatatccta ttcctgataa ataagctaaa tacctgtcaa ctaatggtcc attatcagca
    18001 aataacttgt atttaaaatc tgttactgtt ttagaaatat aatcacaaat ttgttgtaaa
    18061 aagtccttta ctacaatgtg ataatccttt ccatgacaat attttgataa atttgaattt
    18121 ttattctttt cttgtctatt tatatgatat ggaaatgcac agactattat agagttagca
    18181 tccttcatta tatgtcttgg gttaattctg ctctcaatta ttggttcttc cataccagtt
    18241 aaataacctt tttgttgtct gttcttaagt atcttttcta aattatcata tttatctacc
    18301 cctgctactc ctacacagtt taatcctatt aaattacaaa atgcttttaa ttccttatta
    18361 tccataaaat tattcctttt taatctaatt gactttttaa atttatttaa tttttttagt
    18421 ctttttaacc ttttctagct actaactctt ttaaatactt aatcttttta gctattcaat
    18481 tttcttacct gcttaactct tttagatact tcgtcttttt atctatttaa tttttttacc
    18541 tactaactct tttaggtact tcgtcttttt acctatttaa ttttttacct actaactctt
    18601 ttagatactt catcttttta cctatttagt tttttaccta ctaactcttt tagatacttc
    18661 atctttttta tttacttatt taactttttt acctactaac tcttttagat acttagcctt
    18721 tttcatttac ttatttaact tttttaccta cttaactctt ttagatactt agccttttta
    18781 atctattcaa ccttttttac ccacttaacc ttcttacctt ttaatataaa ctctaaaact
    18841 tcaaatatca ttagttatat tttgacattc tttctatagt taagcaaaaa gattgatttt
    18901 ttcttataat taatatccca tttacattgc catttattaa gtctttactt gacttatatg
    18961 gatttctttt tactctcttt actaaactta tacttattaa tattatgaag gtcttataat
    19021 agtctaatat gaatatgccc atcatatatc aaattttaaa atttgtaagc aaaatactta
    19081 tcttatatta ctacatctaa agtggaaaat tttacaaaca agctctgtaa aaacacttta
    19141 aataacaatt tgcttttaaa tcatatacag attataataa atactgtata aggatggtga
    19201 aaaatttgat taaaataaca attccaggta gacctattag taaatctaat tttaagcttc
    19261 ataacataaa tggtcaagca tggatgcctt caaagggaaa atattctaag tatcttgcat
    19321 acgaaaacat gattgcaggt tttataaacc aacagtatca aggggaaact gttgaagaaa
    19381 atctaataac agtcttgaaa ttattcttcc caaataagag aatgggggat ttacataatt
    19441 atccaaaaag tatttgtgat ggtattgaaa aaagtggaat aataaaaaat gataaacaac
    19501 ttaagccagt acttttattt gattttatag ataaagataa ccctagggtt gaaattgaac
    19561 tttatccagt atctaaatac gatatatcct ataatatttt tgaaaaataa agattttatg
    19621 tgttattcga cattatattg atactccagt atttgaaact atactaatac aacaataata
    19681 attgtgttat gtctatactc aaacaaatgt ttttaaaaag cattaagtaa atatttaata
    19741 agtattaaac aaatattaaa tttatataat agctataact atgtaaattt aatatttgta
    19801 tataagacca catctaataa aaagaaatcg gagtaaattt aatacaccga tttcaataaa
    19861 taaaatctat cctagctttt caacaacagt cttaagcatt tctcttatag gaagttcata
    19921 tggacatcta ctctcgcaaa gcccacagtc aatacactca gtaggtttaa catccataac
    19981 ctcatatttt tctttagccc actcttttaa tccatatcta gtataataac cttcacataa
    20041 aaaactcaat ggtatattta ttcctacagc acaaggcata caatattcgc atcttctaca
    20101 gaatttttta cctaaagaat ttcttatttc ttcaatttcc ttattatctt tttcatctag
    20161 cacaagattt tcaagtacag cgacattttg tctaacctgt tcaacactct ccatacctgg
    20221 tataactaca tctatgtaat ctttagatag tatatattta atagccagtg ttgcattgtc
    20281 taaagcacct ccagctaaag gtttcattac tattatccca attccttttt catgagcttt
    20341 tttaaacact tcatctgctt gtccttcaac tatattgtaa gggaattgta tagtgtcaaa
    20401 ttttccatct tctatagctt tttctatagt atttaaattg tgactagtta taccaatatg
    20461 ttttatctta ccttgctcct ttgcctctaa aagggcacta taagccattt tatctttaaa
    20521 aagattatca tactcctctt ctttaacatt atggaattga tataaatcta taaaatcagt
    20581 tttaaaatta tttaaactta tttcaacatc tctcttcatt gaatcataat ctctggacat
    20641 acttttagta gctaagaaaa atttatctct ttttccttca atggcaattc cgattgcttc
    20701 ctcacttatt gtataccctc ttgcactatc aataaagttt attccttgtt tttctaactc
    20761 atttataact aagttagtat catcttgagt aatcctttgt attggaatgc ccccaaagcc
    20821 aactctcttt attttcatgt tagtattgcc taaatttctc atatctttcc ccacctaact
    20881 tttaataatt ttttatataa tataaatatg tcatctttaa ttatacaaca aattatgaga
    20941 atctttgcaa tatatttaca tactattcat agtataatta tattttatat cagtctattc
    21001 ataatataat catactttat acccagttaa tttgtataca atttttgtta ttcctatcca
    21061 ttaatttaaa ttatcacatt tcatgattgt atctccaccc taattatgtt gagggtgaag
    21121 tatttcacat atttttaata aaggtttatt tactagatag tatttctttt atcattttaa
    21181 gctctttctt gtgaccttca tcatcaaaag gagtttctaa gaaaaatggt aaatgtttta
    21241 gactaggatg ttccataaaa tcaattaatg cttttaatcc tatctttcct tcccctatag
    21301 gagcatgtct gtctttttta tctccaaatg gcatcatact atcatttaga tggacagttt
    21361 tcaatctttc tatgcctata accttatcaa attcttccaa aaccccatct aaatcattca
    21421 ctatatcata accagctgaa aaaatatgac atgtatcaag acacacacct attttttctt
    21481 tatgctcaat tttgtctatt atacttttta attgctcaaa tttaaaacct atttcagttc
    21541 cctttcccga catagtttca agaagtatag ttatattttc atcacccttt atggcattgt
    21601 tcaatccttt tactattcta tctatgccaa aatctacacc accacctaca tgacttccag
    21661 gatgaaaaca catgtattca attcctaatg agtccatcct tcttatatcc tcatctatca
    21721 cccttgttgc aaattcgtat acatcatcct ttgtaccacc cagattcata gtatatggtg
    21781 catgtgcaag cagtggtgca aaattatttt cttttctaat ttcttgaaat ttaattatat
    21841 ctttttcatc aaattcttta gcatttcctc ctcttggatt tctactaaaa aattgaaatg
    21901 tatttgcacc aatatataca gctgtctcag ccgcattagc aaatcctttt gctattgaaa
    21961 gatgtgttcc taatttaatc atattaatct ccttttattt gtactactac ttgtactcaa
    22021 ttttaatttt gaactactcc accttaacta catttagcca acatatgaag atggagattc
    22081 ctatttgata gtactttttc actaaaaatt cgtaagctct aagggatgtt ctatcccaac
    22141 taatctcagt cctcttttaa catatttcta gcattgattt aatttatatt aatttttaat
    22201 tttaatattt acactttaat tatacccatt ttaatcaatt tcaaatatta taatcttaca
    22261 tactttgtat attatctaag cacttcttag atagtgtata catcttacac tatacatatt
    22321 atttacaaat acaataggta taaaaaaaca aaaaaataga gattaaaaat cattgtttta
    22381 cccataccaa aaactatttt ttgattatta agtaaatttt gatgttcaat ctctaatatt
    22441 atatctttaa ttaatccata attaattctc taactaggac attcctcctc aagagtagga
    22501 actataacca tagctatatc atacttagaa ccttcaacat tgtgttgtct tttatttaaa
    22561 atttcatcta tctttgactt catagtgtca ctaagttcaa ctaattcatc atgagtaaga
    22621 gaaatatcat gataatcaac tatattttca ttttctaaaa catcttcctc tgctgcctta
    22681 taaaaaactt cactcatatt ttcaaatttt gatatgtaca attcttcttt ttcttcccca
    22741 ttgtctaaag aaaaattaag tatcctattc cctataacca cattttttgc acttggataa
    22801 tagtactttt ccacaatccc cgactttatt ttttcctcaa ctaattctaa aataccaact
    22861 ttgtataaca tttttatatg gtagtttatt tttgcatgag gttcttccaa taattgagat
    22921 aattgttttg ctgacaatgg cgatttgtca aatgctttta gaatatctat tctacgtgga
    22981 tgcgcaatag cctttatgca ctccaaatct cgtaatacca atacctcttt cattgacccc
    23041 tcctaaaaaa tttatttaat tcatatttct ttacaattta attatatatt aaattatatg
    23101 ttgttgtcaa aaaaatcctt tcttaatatt ttaaaaaata tttaatttga gtttatttga
    23161 caactcctct aaaactttaa ctcctgcgac gctgttacct tttttgtcaa gcgcaggtcc
    23221 ataaacacca atccccatgc gtcccggcac agaagccatt ataccacccc caactccaga
    23281 ttttgcagga atgccaactt taatagcaaa ttccccagaa gcatcataca ttccacaagt
    23341 catcataaat gtcttgacaa tcctagaaac catttcactc attaaatgct caccattttc
    23401 tatatctact ccataatttg caagatttat tccaattctc gctaaatcta cagaatctat
    23461 ctcaattgag cattgtttaa aataaaggtc tagaacatct tctacattcc ctctgataaa
    23521 tccatcactt ttcaataagt aagccatagc tctgtttcta tctccagtca ttttttcaga
    23581 cttatagaca tcatagttta ttcctatatt atcatttttt gctaactttc taaagaaact
    23641 aagcattctt tcttcttttt cttctaaaca actcccattt ataagggaag tggttactat
    23701 agcccctgca tttatcattg gattacaggg tttttttgtg tcattaattt caagtttcat
    23761 tattgaatta aatgggtccc cacttggctc cattccaacg tttgaaaaaa cgtcttccca
    23821 atcattatcc ataagtgcca tagcaagcac aattggtttt gaaatacttt gtatagtaaa
    23881 ctttgtgcta cagtttccag cactataaag attattttcg ctatctatga tacaaattcc
    23941 taaatcattt cttctagcgt tttttaattc tgggatgtaa ctagcgactt gtccataatt
    24001 agtatacttc ttattagaac ttataatttc ttttaataat gtctcatcta acgtattcat
    24061 atgcacctct tatattatgt tatatattct attttagcac aagtttatac tataataaaa
    24121 caatgttaaa aaaaattata ctttaaaatt attttctaca agaatcaatt catttttcaa
    24181 acaatttaaa tagtttaaag agcttaataa tttagtaatt caataattta acaacctagt
    24241 aatttagcaa tccagcaatc taataatcca gtaatttaaa tttttataac accaaaaatt
    24301 tctaacccaa caccttttac tcttttactc ttttactctt ttactctttt actcttttac
    24361 tcttttactc ttttactctt ttaaaaaata atcacataaa ctaacaaaat attaaacaaa
    24421 aaagtaaaat ttaaaataaa aagcttgctt catacaaaag tataaaacaa gcctttttat
    24481 atatcaactc aaactttaaa atctgtatca atttaattct ccactttaca cttaatatac
    24541 ttaacataaa ccaataacac tacttgctat gccatttaat cttcataaaa caatctataa
    24601 tacacacatt aatcaactat tttacttcct ccaataaatt ccctaagaat tgaaatacct
    24661 ggtatgtcag aagtatcgtc caattcaaca aaatactgca aaaactttag taatctagta
    24721 gcttcaatat gtgcaggatt atcttccttt atctcttcca acaaaggttt cgcaaatcgt
    24781 tttaaaactg ccaattcata tacacaagca gagttaaata tagcatctgc ctcctcttgg
    24841 tacgggaata tattcttctt ttctcctctt ctaactgatg cccattgtaa aatagtatta
    24901 tcagcactat atcctctaaa attatagtct ctaacaattc ttcttatcat tctcaaatca
    24961 gcagtaggga ttcgattatg gtcatccaaa tttatttgag taagggcact tatgtatatc
    25021 ttaaatttat cttcatctgg tatagatgat gttaaaatag gatttagccc atgtatacct
    25081 tctaatataa ttggctgatt tgaatctatc tttatctttt taccagtgta ttctctctta
    25141 ccttctttaa aattgaatct tatatcatca atttcttcac cagctaaaag tttctttaag
    25201 tctaagttaa acttctctaa atctattgca taaattgatt caaaatcata atttccatat
    25261 tcatctaaag gagtatgttt tctatctata aaataatcat ctaaagaaat tgaaatagga
    25321 tttaaattat ttactctaag atgtattgat aatctgtgag caaaacttgt ttttccagat
    25381 gaagatggag ctgctattaa aactactctc ttattgccat cctttatcat atccgctatt
    25441 tgagatagct tcttttcatg caatgcttca aatgtcctta ttatatctcc atattcattg
    25501 ttttctatta ttttattaag agatactacc ttgtcaattc ccataagtct tgaccattct
    25561 tctgcttcat aatatacact tgatagctta ggttgaggta caaatttcat aggaagagtc
    25621 ttatcttctt cactaggtcc taaaattata acgcctccat tataaagctt aatatcaaat
    25681 gattttatat aaccagttga tggcaacata taaccataaa aatggtctat atagtttcta
    25741 cacttatata ctttagcact atcatactct ttgtatttca aaagctctgc cttttcatac
    25801 atttcattat tttcaaagat agaaatagct tcatttcttg taacttcaat tttttctata
    25861 acataatcgt tgtttactat ctctttcatc ttgtttttta tattttcaac atctgcttct
    25921 ttaagctttc tatctatatg aacttcacaa tatagcccat ctgatagtga atgttctatt
    25981 gtaactctgc tatcccaaaa gatttctcta catgccatta caaatataaa tgacatgact
    26041 ctaaagtaaa ctctctctcc atcttcatta gtagtatcta aaaaatttat ctcacaatcc
    26101 ttcttaactc tacaatttag ctcttttagt ttattgtcta caactgcaag tgttatataa
    26161 cccttatgct catcacaaaa ttcttttgct atatctccca atctgattcc aggggaattt
    26221 tcttctaatg aatattcctt atcttttcct tctattttta agtttatttt tttcacttca
    26281 acatctcctt ccttttgatg aatataaatt ttaattaatg aggcatatca acaagataaa
    26341 gagaggtatt taaccctctc ctacaatatt attcaattta cttatagtat cttcaatgtc
    26401 ttcgtaattt atttctaata ctaaatattc tgcatctaat gttaagacct ctttaatccc
    26461 ctcttcagat aaaattcctc ttcctatacc aacatgtgta tcattgatac catcattatc
    26521 acttaagtgt atcagtctta ttttatcctt taagttcttt acataagcat cctcatcaat
    26581 taaacagtgt ccagtatcgt aacaaaaaca aatcttagaa tttttaatcc tcttaaatat
    26641 atattcaaaa tcataagata tatttccaat atttgaaaaa tccccacctt ttgaataagt
    26701 attctctata catacatatg aatttgtatt taatttatct aaaaaatcca cactatttcc
    26761 taaatatttg tctctattct ttctaactct atttgtcata acatatccca aatgaaggtt
    26821 aaaatacctt aaatcaaatc cttttagttc aaattctatc ttctcaataa aatttatcca
    26881 tgaatttcgt atatactcta tgttttcgca agtatttaat tccataggca agtgtatccc
    26941 tatagataat cctaacttag aaattctctc tttatattta cataactcac tacattcaga
    27001 tatattgtca attccaatct ctaaatgagt tatttgcttt atactctcac aaatgtttaa
    27061 agcctcctca atattaaaca ataaggacgc tataccaact gccatacaaa tcacctactc
    27121 aaaaatacct atatctgtaa aaggaaatac tcttaaaata acttttcctt taatattttc
    27181 ttcatctact aatcccactt ctttatatct actgtctaaa ctaacttctc tattgtctcc
    27241 catcgcaaat acttttccct taggaatcac catatcaata tcgccttcag tataattatc
    27301 atgtatatat gaaacttcat ttaataactt accatttata taaaccttag aatccttaat
    27361 ttttagatgg tcaccaggaa cacctataac cctttttaca agctcttttt tccttccatt
    27421 ctcttgtaat aaatcagtct taaatatcat tatatctcca cattttggtt ctgataactt
    27481 atatgtcatc ctgtttgcta ccaaataatc atgtgattta agtgttgggt acattgattc
    27541 tccatttact aatgttggtt ttataaaacg agttataata aatgcaaaaa aaagtgcagt
    27601 aataattatt tttatccact ctacaatctc ttctttaata gtttcattca ttcacaaatt
    27661 ctcctttata gttatattat ctttattgat aatgtctctt aaacactatc ttttagataa
    27721 gtctactaaa taaggtgttg tcttttttat atagacaact attgtgtatg gtctggtgat
    27781 ttcagctaat cagcgattgc tccaaatttt atatgtttag tcaatagttc ctatcttaga
    27841 aaatggatac aatctgacta gaacctttcc taaaatggta tgctcgtcga ccaatccaac
    27901 ctcatcgtat ctactatcta aacttttttc tctattatct cccattgcaa ataattttcc
    27961 ttctggaact acgatatcaa tatctccatc agtacgatta ttatgtatat aggaagtctc
    28021 atctaacaac ttatcattta catatacctt ggagtcttgt atttttacat ggtcaccagg
    28081 aacacctata actcttttta ctaaatcttt tttctttcca ttttcctctg ttaagtcggt
    28141 tttgaatact attatatctt tgtattttgg ctctcctacc ttgtacgcaa ttctgttaac
    28201 tatcaaataa tcacgttcaa ctaatgtagg atacattgat tctcctttga ctattgttgg
    28261 cactataaaa cgagttattg caaatgccaa aacaagagct atgacaatca cttttatcca
    28321 ttctacaact tcttttttaa ctgcttcacc cactaaaatc gtctcctctg tgttaaatat
    28381 taagtcaatc tacagcaaaa taatctacca taaacctaca atataaatat tatacttctt
    28441 ttttctaata gtttaaaata cttttctaag acctttagga taatgattct taattacacc
    28501 agaagattct tttccccaac ctaaaggtat tccttcaacc gaaaccaaaa cccatcctct
    28561 actatcatta gaatttacaa catccccttt taaatattta agaattgatt cactctgtac
    28621 actgaagttt tcaacatttt ttacatcttc aacttttaag taatgagata gtgcatgaga
    28681 aggctcaaat ctattcttct ttaaaacccc taaatgcaaa ccatatctca atacttttag
    28741 ttttttcgta tctggatata tttcaggaag taggtacaaa ttttctcctc ttaaataaaa
    28801 tttatttttt ttattactat aaaaatcaac ctttaaaaat ttcttttcaa actccctata
    28861 atcctttatt tctttatcta aattttttat ttttaattct ttgacattac attcttcttc
    28921 ttctaatttt tttattttgg caacaaaatg accttctccc tttatcttat gaggccataa
    28981 tctttccatt tcaataagtt ctgcatgtgg atattcactg ataaactcat ttataacatg
    29041 ctcattttcc tcccttgaaa aagtacaagt tgagtaaatt aaaatgccat ccttctttaa
    29101 cattttatat ccatctctta taatctcttt ttggatggat tgacattcta ctacctttgc
    29161 ataactccaa tctgatatgg caacctcatc cttcctaaac ataccttgcc cagaacaagg
    29221 tgcatctata ataaccttat caaaataccc attaaacgtc tttcttaagc tattagaatc
    29281 ggtgtttgtt ataatacaat ttctcgctcc aaatcgctct aaattttccc ctagagcctt
    29341 aattctacta gcattaattt cattagaaac caataaacct gtatcgttta gcttagatag
    29401 tatatatgta gattttccac caggagcagc acacatatcg agaacttttt caccctcttt
    29461 aatatctgcc tttggtatta cactcattgc agaaggctct tgcaaatagt atgctccagc
    29521 ctcatgcagt ggatttctac caggtctgtc aattttatca tcataataaa aaccttcttc
    29581 tgcccatggt atttgattca aatcaaacaa ctccaaatca aataattttt tcttgtcaat
    29641 ttttaaagta tttattctaa gcccagtagt cttactttcc tcataacttt ttataaaatc
    29701 atcatattca tcttttagta tttcttcaat atcttctaaa aattcatttg gtaattctgt
    29761 caaatctata atcccccttc aatctgcttt acttgtgtgt catttattat actttaatta
    29821 tttatgtctt tcctttaaat tttatttctt cttctttttc ctaagattac cgaaaaacac
    29881 ttttttagag tgattcatat atttctcaag tttatcaaga tttatcttcc acaaaattat
    29941 tacataaact ataagtaata taatagccca tctatattct atcatcaata tgaaatcaaa
    30001 aactccatgt aataaaattg gaattaacac tgccataaac aaatactcac gtcttttatt
    30061 tttatttccc tcaaatttat acttagaaat atagtagccc atagtaattg caaacattac
    30121 atgagctggt atagagataa ctgccctatt aataccaact tgcagtgcta gatttctact
    30181 ttcagaaaaa atataaacca tattttcaat tgttgcaaat cctaaagcta aaaatacaga
    30241 gtaaataata ccatctaact tttcagtaaa gtgtttttct ttaattaggg caggaatcag
    30301 tatcagagct tttacacctt cttctacaag tcctgcaact acaaatgata catatattag
    30361 ttctgaatac ccttcaaata cattaaactt catcagtaaa ttttctaaaa gtataattat
    30421 aacacttaga aaagcaccta ttaaaaaaaa tttaattaga tggattaggg gttctctaca
    30481 atatctatct ttagacctta tccaaaacat tcctatcaat ataggaacta ttgctaataa
    30541 aaataaatct agcttcatag attattttcc tttcaaaatt tttgatttat ttatgattta
    30601 tatcatctac ttatttttaa cttattcgtg ttttaaatac atactaaaat atacattaaa
    30661 ttattttaaa ttatttaaat acaaatatac tcaaatactc aaaaaaattt aaatttatat
    30721 ctatattatt taacaaattt taagctatat tcatatcttg aaatataagg gaactaaatt
    30781 catttaaaga aagggtgatt tgatgcaaga tggtttctta acagtaagca taattgatgc
    30841 tactaacaat agacctattc aaaatgcagt agtaaacata tattcaatgt ctaatggtag
    30901 ccaaagctca tcaactttat accaaaacct aagaagtaat gaatctggtc aagtgactgg
    30961 tttagtatta ccagcaccag atgtagatta ctctctacaa ccatctgatg ttagacctta
    31021 tagccagtat atagtggaag ccatagcaga tggatatgag acagttgtca tagaaggaac
    31081 acagttgtta gctacaattg aagcaagaca aggagttccc atgtctccta gaacaagaag
    31141 taagcgttct ttctcaagac aaagtgagtt gatttttgac ataggagaac atactctata
    31201 tggtacttat cctcctaaga ttccagaaag taacttaaaa ccattaccac ctcctactgg
    31261 tttcgttgta ttagataacc ctgtagtgcc tgaatttata gtagttcatg atggtttgcc
    31321 agaagattca tcagctccaa actattggat tccatttaaa gagtacatta aaaacatagc
    31381 atcatctgaa atatattcaa cttggcctga acagacaatt tatgcaaatg taatagctat
    31441 aatatcattt actttaaata gagtctttac tgaatggtat agaaataaag gatataactt
    31501 tacaataact tcaacaacag catacgacca taagtttata aataatagaa acttatttga
    31561 accaattaac gtagttgtag atgctatttt taatactttt ataaaaagac ctcctacatc
    31621 aagacagcca ctattagcac aatattgtga tggacaaaaa tcccaatgtc ctgaccaaat
    31681 gactcaatgg ggcagtaaag acttaggtga ccaaggatat gactatgaga gtatactaag
    31741 atatttctat ggagatgaga tagtatttga aagagcgcct atagtaagtg gtgtaccagt
    31801 gtcattccct ggtactacac tacaagttgg ttcttctgga cagtatgtta gaaccataca
    31861 aaatcagtta aatgctatat ctaattctta tccagctgta ccaaaagtta tagaagatgg
    31921 tatctatgga acagatacag aaaacgctgt taagatattc caaggaatct ttggtttacc
    31981 tcaaagcgga gtagtagatt ttaaaacatg gtatgaaata tccagagttt atgtagctac
    32041 aactagaata gcttctttaa atcctttaat ttaaagcttg atttagaaca taaactcaaa
    32101 ttattgaata aaattattaa tttaaaataa aaatggctgt tatgaactaa tatataccat
    32161 aagtattact tataagtatt gtagtcttaa cagccatttt ttagttataa cttgtttttt
    32221 tagaatctta aaactttaaa tgggggattt taagcttctt aaaaacagtg cttagcaact
    32281 tagatttctt taaaatatta tatttatttt taagtttaat aaaatgctat tctaacgaaa
    32341 aacctacttt ttatttaaaa tctcacttat tatttaaaat gctacttctc acttagagta
    32401 ttactcctta ttatttaaaa cactatctat cacttagagt attacttctt attatttaaa
    32461 acactatcca tcacttagaa tattacttct tatataaatt attatttatt ataaatacaa
    32521 ataatattaa tttaaaaagt tattcctaat ttaaataata ctactatctt catcagtgaa
    32581 cctaaactgc aactttagac ttattagcta aagccaatat tagacctaaa gctatagatg
    32641 caaatgcaga agcaattact ctatactcag cagggaataa gaatacaatt gtatgacatg
    32701 gtatccagaa aaatggtata gtttttaaaa cactaaaacc aaggtaagca ttccagtcca
    32761 caatatttac cacatcttta agttttacct taatcccctt tccaacctca tattttttat
    32821 ctataaaaca gtccataacc ttatggaata aaaatacagt tggtgacatt agtatattca
    32881 tagttgcact cttaaataga gcatttgcaa aagctattcc ctcaaatgga agcataccat
    32941 ccacttgtgc ttgacctaca cctgatgagt agattgacat tccatatgtg gtaactacac
    33001 ctattattcc ccaaactatt gctctataaa atactcctac tggtattttc caatccttat
    33061 acaaaattct agcaccaagt aaatctccca ttgtagctaa tacagcaaac tttaaaaaac
    33121 ctccaatata tggatgtgca cctgttgctt ccataaatac ttctctacta cttggtattg
    33181 ctaagacagc aataatacaa ataagaactc cactccatat aaaatcgcct tttttcatat
    33241 ttataaatac tatttaatat ataaatagtt ctcctttcta ttaaagattt atttaattta
    33301 caatctatct acaattattt attaatcact tattattaga tattcatttt ccatatttaa
    33361 atttattcta tccctttatc tatttatttt cttactactc tatccaccat tatctatata
    33421 tttattttac gattactctt tcaccctaac cctttaatta tctatttttt ctgatattct
    33481 tatctaccat catccatata tttattttac gattactctt tcaccctaac cctttaatta
    33541 tctatttttt ctgatattct tatctaccat catccatata tttattttac gattactctt
    33601 tcaccctaac cctttaacta tctatttttt cttactattc ttatccacat catccatata
    33661 tttattttac gattactctt tcactctaac cctttaacta tctatttttt ctgatattct
    33721 tatccaccat cacccatata tttattttac aattactctt ttaccctaac cctttaacta
    33781 tctatttttt tctggtattc ttatctacta ttatctgtat atttatttta tgattactct
    33841 tttaacctac cccctatact ttatagtaat atgtttttaa cctttttcat tattcccaat
    33901 atgctttata actacaaata ctctcccccc ttaaaaataa taataaaatt aatcaataaa
    33961 tttataatca tattattact ctatgtatta agcaattact atgccaataa actttgattt
    34021 ttttaaacaa atcttaaaaa tcctaaaatg ctaattttct ttaactttta atgaatctaa
    34081 tagataacat ttaattttcg ttatattttt cagaatattt ctattattat ttttaagaaa
    34141 aaaaaataat gttaacattt cttaacatac attaaaaaat gttaacatta taaattgggt
    34201 ttaatacttt tactttgact cgatttactt ttattctact tcttacttta tttttaactt
    34261 agactttatt tttttattac ttatttgagt cttatttatt ttattatcac tccatatgtt
    34321 caattacatt tgtctaaatt ctttgatact tttattacat aattttaaca agcttaagtg
    34381 ttatttgggt agtgcagttg ataattattt aaataatcat caactgcatg tttatatcat
    34441 tacagtagct taatgtattt aatcatactt acttaggatt ttgaaatatc ttagagatta
    34501 tatttaacat tagtcaattg ctgcataatt gtaagcaatt gaaatttgag acccatcttc
    34561 attttctata gtgataggct tcataatttt taatccttca gtttttatct tttctaattc
    34621 ttgttccatt ttcttaattg tttcctcaag gtctttttga cttacatctt tgactgcatt
    34681 agggtcttta gcatagtctt ttaattcttg aatatgttta gcatactctt cataagtata
    34741 atgctcaaca ccatactttt ccaattcagt agggtcagtt attcctgatt ttattagaga
    34801 ttcaagttta ggtaaagcaa tattagatga atcatttcca tttgcaaata ctgctccagt
    34861 tcctcctagt accattgcag ataccatcat tgcacttata agggattttt ttattttcat
    34921 aaccaatctt cctttcttag agatttattt gggtagtgta gtttgaaatg actattgcaa
    34981 tagttatcaa ccacatcctt agactatcac agtagtcatg tttatgtcaa gcctttttaa
    35041 aaaacttttc aataaaaaat actatctcaa aacaaaatta attattttga gatagtattt
    35101 taattctaaa tcttttatat tactaagatt tatggttact ctatccatgt tctatattgt
    35161 tctttattaa ctgctaacaa attccctttt cttgatatga caaagcgatt atcattatag
    35221 ccactttgct caattgaaac ttctcctgaa tcagtataaa gtacatcctt accattacta
    35281 gatttattta catacgtttc ttcgctattc tcagaattag ttactttgct catagatgat
    35341 tcttcatgct ctctaaattc aattacagat acttttgccg tactaaaatg agtctctcca
    35401 tcacttttag ctttactaac aagaatatca gattctactt tgggagaata taaatcagtt
    35461 gatttactac ttattgaaaa tccaatttta tcactttgtt cattttttgt taaaccaaaa
    35521 gattcaacct cattaatctc tttattttct gttgcaaaaa caagacctgt cccacaaact
    35581 aatacagttg aaactaaaaa agtaacataa gttgattttt taaatttcat aatattaact
    35641 attctctttt ctatttttgt ctttccaaat ccactacata ttgtcattga aaatggatta
    35701 ttctcctctg ctagacttat tagtgtggtt gcataatcag ccctatattc ttcaccaagt
    35761 atactcataa ccttttcatc acaactaaat tctatgtctt tatttagtaa aaaaaacatc
    35821 acccaaacta agggattaaa ccaatggaca acaagagcta caagcataaa taactttaac
    35881 agattatcaa agctattaat atgaaccatt tcatgagtta aaacaaagtc taaagtttga
    35941 ttattagcat aattcatttg ctttggcaaa ataattcgtg gttttaaaat accagatgta
    36001 agtggtgtcc tagtaaattc agatatgcaa acaataaatc ttctaaaacg gctattctta
    36061 cacatccatt ttttaatgaa ctcatgattt tttatatgaa ttgaagtttt aaaaagatta
    36121 attattttaa aatatctaaa gcataaaagt atgctaaaca taatagtaac tataaaagac
    36181 aaaatgccca acatttccaa catcataata ttttcaatat tagacattat ttcaaattcg
    36241 tcagaattgt ttttacctgt agagtcacta gctttcttaa gagtataatt ttctgttgat
    36301 gtattaccca attccacatc aaaagtcgat tcaattatac tataaatact aaaaccagaa
    36361 tttaaactta ctggtattag aagtttaaat aaaacaatca accacaataa tacaaatacc
    36421 tttttaggta attttttaaa taaacatgct cttataaaaa taactattaa aagtaatact
    36481 ccagaagtaa aactcatttc taacaattta atgaacataa atcttacctc actttaattt
    36541 ttcaacaatt tgtttaagtt ttgtaatctc atcacttgaa agattttctt tattaataaa
    36601 agctgaaaga aatttttctg aagaccctcc aaacatttta ttaattaaag tttctgtttc
    36661 ctgtttttgt acttcctctt tgcttattaa agctttacaa acaaaattcg gctcaattct
    36721 ctctattgca ccttttgaca ctaatttttt tataacggta tacgtagtgt ttctattcca
    36781 tcctgtttct tcttttaaaa tttttactaa ctctccagcc ttgctttctc ctctctccca
    36841 tagaacttcc attactttaa gttctgaatc aaataatttt atctccatat aaaatcgctc
    36901 ctcaaatata gttatcacaa tagtcattac tataataaaa aactatcata gtagtctata
    36961 ctatgtcaat catttatttt ttaataataa aaactgtctt tttaaaaaat tcttataaaa
    37021 gacagtttta tcaatgttat actttaatct ttaattaaag ccatatttta tttaaatata
    37081 taactatata tcttagtaca tagtataaga tttttcttta tctaatagta tatcctctac
    37141 atctgggttt tcttttacta attgtctata aaaattacaa ttagtaacac ctacatatgg
    37201 aattagccat agtagaccta ttccaagagt agctattgaa agtatatacc atcctataaa
    37261 actaaagttt agtacaaata atttaagtac atttcctttc atcatatcta aactagcacc
    37321 cattgattta aatacaccca tattatcctt gtcttcacaa attaagaaag tagcttgaga
    37381 aacatataat agcaatatat aaaccggtaa cataagaaca agtattataa gaactagagt
    37441 tattccagta gaaataacag tccctattcc aacattatca cttgataaat attcaattgc
    37501 tccactagta agtaaaactc caaatgataa aaacgctcct ataattgtga ttataaaaga
    37561 ataaatataa acaataattg attcaaataa ataaaaacca aaacttctta aataaattct
    37621 cccacttaca aagatatctc ctatcttgac tttttcatct ttagtaattt tcaagaacat
    37681 acatgtaaat cctgctgaaa cgcaaaaagc tattacagcc actacaatag tagtcaatgg
    37741 ttcaaaaggt aatcctacaa ttgatattaa tactatcaat gcaatatata ctgccataga
    37801 aattgcacat atcccccagt ttccagataa ctgagcttta gatactcgtt ttaattcttg
    37861 ccttgatacc ataaaaaaca cccctttcat tttataaaaa aacttttaaa aatataaata
    37921 atcttttaac gtataatatt aatatatcat attttcgact atataagata caattattca
    37981 ccatgtttta tcaagatata ccataattgt attactagtt ttatatagtc aacattacca
    38041 aatacatcaa ataaatttga ttaattattc aacaagtaat aaaataattt ctattaatta
    38101 tactttattt agtaaacaaa ttatttaata aaaaaattac ctaatacagt aaattaattg
    38161 gtaagataaa ttatttatta ttagtaaagt aaattattta ctatttatca agataaatta
    38221 tttataaaat aaatcattta taaaatataa tttatacttt gaaaataaaa taaactatct
    38281 tccaaacatt ttattatatg cttaaaagat agttttgtgt ctttaaaata aattaaattg
    38341 tatatattcc ttcacataac ttttgtatat tactaggtgg ttgccattta ccatcttttt
    38401 tagtatatgt aatcatagtt gtaacttcct tagttggttt cttattgtaa aggtctataa
    38461 tcatttttgt catatcatct tgaacttctt tttctttttt atcgtaattc ttttcatcag
    38521 ttacataaga ttttactaaa gtgttcgctt cactcattac tttactagta tctaaagctt
    38581 tacttacaac tttcaaagtt atcttatcct tactagaaga ttccactgtt actgtctttt
    38641 caacaccttg aattaattta acaattgttt cgtatatctt ttcagcattt tctttaccat
    38701 aaagaggtgt caattgagca atcattgaaa ccttgtcctt gctgttataa gtttgaatct
    38761 gtttatattc agcttctgtt aatgcatcag aaattccttt ttcatcatac ttatacattt
    38821 ctaatgcagc taaattagct tcaaaattcg actttacttt ttcttctgca ttagaacacc
    38881 ctatagttag aactaaaact agagccataa tcgtagataa tattaacttt tttaccattt
    38941 tcatacaaac atcctccctc aattaaaata ataaaaaatt tatttatcaa cattataaca
    39001 taaatttgtc taactataga attattttga atattttacc acttccagtc tattccaata
    39061 ccgttttctt ttgctttatc gtatattctt tttgctgtta cgacatcttg tacacctatc
    39121 ccaacagttt taaatacaat tatctcatca tcatcttctc ttccaggaat agttccgttt
    39181 ataacattcc ctaaatcacc actaaaatca tctttactta tcttaccttc ttttaatgga
    39241 attaatatat ctcctgactc agataaaact gcatctgttg attcaaaaaa tatcttagac
    39301 gctctagtta aacagtttgg gcacaactct tgcatatctg gcatatatga acctactgca
    39361 cttattaaag ctcctttttt cactaagttt ccattaagta ctggttgttt tgatggagta
    39421 gctaaaacta tcacatctgc gttatttatg gcttcatcag atgattcaac tgcaactatt
    39481 ttagtattat atttacttaa ttttaagttc atttcttctg caaatttttc tctactttcc
    39541 ttgtttctac taaaaactct tacctcatcc aaatttctag cttctatcat ggcttcaagt
    39601 tgtggctcac cttgtcctcc tagacctatc aaagctccaa tcttagaatc ttttttagcc
    39661 aatacgtcta tagctgctcc agaggctgct ccagtacgaa tctgagttac ataagtgcca
    39721 tccaatactg cactaacttc tccagtttct ccatctaata atagtactgt tgctggaatt
    39781 actggtttgc ctttttgagc atttttagga aatacagata caatttttat tcccccacag
    39841 cctaattctt ctatgtatcc tggcataaat aacatactag catcttgact tggaacagat
    39901 atattggttc tcaaaggatt tacactcttt ccttcacaat aaattctaaa agcttcctta
    39961 tcagcttcaa ttgcatctct cattgtaaat acttttttta tgtcgtcttt ttttaaaagt
    40021 agcattttta tacccccaat tttaataagt ctctatgcta aatattatag cataacattt
    40081 aatttaatct tctatttcaa atcaaataaa tctacatact ctggttttag catagaactt
    40141 atcaacgctt tatttattga aaattctggt attcctgctg catatggagt tatatcatat
    40201 aaatcaaaat atacaactag attctcatct tgcaaataaa agttttgatt ctcttttata
    40261 cctttaaaat tatatattcc gatattttct tcatcctttt tttcaagttc agctatttgt
    40321 cttttaattt cttcatctat aacttcttta tatttagtat tatccttaaa caagtccatt
    40381 aattctaaaa attttccagt tctcatatcc acattatatg ctatatcttg ataaaatcca
    40441 tgtgctccac cactataagt ataatatctc acttttatac taagagcaga atcagtattc
    40501 tttttaagtt caaagtctac atttgcaaca aacttatttt tctcgtcagg attatcctta
    40561 agatactgct ttgcttcttt atatgattta ttataaaatt caaatatgtc attttttatc
    40621 ttatcatttg tagacctttc aacaatttta tttgaatttg ttattacagg catgtttata
    40681 gtactttcta aatactcatc atttttcact attgttttac ttcctattgt acttactgat
    40741 aaagtttgtt tactatttgt attacccttt ggtaatgact ggttactcat aacagtcact
    40801 gcttccttat tactatttaa attaattgaa gcatctttcc ttaaattatt atatttatag
    40861 tcatgtttaa ttgccaaaac agccattata gtcaatgtta aaacaaatac atatcttaaa
    40921 actttagctt ttgtatcgac tttaaaaaat ttcatattat cattttcctc ctaaatataa
    40981 atctctctta ttatatttgg agtttctagg gttttttatc catataattt aataaaagat
    41041 taatttttat ttataaaaaa ttaatctttt tgttttaaaa tattaatatg tcttatcaaa
    41101 cagtttgaaa tttcaccaag aggtaattgt aacataacag aacctctatc aaaagctacc
    41161 attggcattc catatactat acaagaattt tcatcctgtc caattgtaaa tgaaccattt
    41221 tctttcattt ttaaaagtcc atttgctcca tcataaccca tacctgtaag tattattcct
    41281 attgaatttt tacctgctac ttctgctact gaatcaaaca aaacgtctac tgatggacag
    41341 tgaccactta ttctttctcc cttttcacaa gttacataat atccttgttt atctgtacac
    41401 aatttcatct ggtaaccccc aggtgcaatt aacacatttc cttgttcgac tctatcatta
    41461 ttcttagctt ctcttacatt cataatacac tgcttgtcaa gccgttgtgc atacatataa
    41521 gtaaatatag ctggcatatg ttgaactaca acaatccctg gtgttgattt tggtaggtct
    41581 tttataattt caagtattgc ttctgtacca cctacagatg cgcctatagc aataaccata
    41641 ttattaaaac gttccacatt accgcctcca aaaaatcata tatcaaaaat acctttttat
    41701 cgtttatata tagcaggttt tacatatttg tatttagtac tacttctgtt gattacttct
    41761 gcatgtccta caaacaaaaa actatctttt ttggtagcat tataaaattt atttataagt
    41821 tcttctcttg tttttaaatc aaaataaatc ataacatttc tacaaaaaat caaatcaaaa
    41881 tgttgatatg agaaatcatt cattaaatta aacggcttaa aaattacact ttttctaatc
    41941 ttatcacaaa ttttaaatgt accatccttg ttgtctataa aatacttctt cttccataac
    42001 tcaggaacat tgttaacatt tgattctatg tatatacctc ttttagcttt ttcaagtgag
    42061 ttagttgaaa tatctgtggc tatgatttta atgttccaca actctttatt atatccaaaa
    42121 taatcatcca aagtcatagc tattgaatac gcttcttctc cactagaaca tcctgcactc
    42181 catatattta tgtttcttat tttattattt tcttcccaaa aaggaagtat actattctgt
    42241 ataaactcaa aatgttgagg ttctctcata aaaaatgtgt ggttagtact caatctattt
    42301 attaggttta tagcttcatt tcctgtctta tctttaaata atagattcat atattgacta
    42361 aaagaactaa gttttttctc tatcattgta ttgtataagc gaccttcaat taacgctctt
    42421 tttttactta aatcaatacc atattctttt tttacatagt ttacaagaac tataaattct
    42481 tcatctgtga gttttatcat gcaaaaactt cctcctgcta atttatcaga actaactata
    42541 tgctattaat caaaaacttt cgaatatcta aaattaatat agaattatct tcaactttcg
    42601 caatctcttt tatatcttta ttaatattag tgttgttaga attagtcgtc tctagtaaat
    42661 ttagattttc tatatgaata acatcaacaa ctttatctac aataattcca acttgatact
    42721 catcaacctt tgtaattatt atacaagttc tctcattata atcagcttta ggaagattgt
    42781 actttatatg catattaata actggtatta ttcttcctct caaattaata actccactta
    42841 tatatgttgg aagacatggt acatacgtta ttgattgaaa tcttatggtt tcaatcacat
    42901 tttctaattc tattccataa ataacatcat tgacataaaa tgttaatact tgtttaataa
    42961 tttcatcttt agccatactt taaatcccct aatatatttc tataatgcta tctgcatcta
    43021 taattaaact tatacttcca tccccaagta ttgtacatcc cgataaaccc ttatttttta
    43081 tattaaatcg gttaaaataa actggaaatg gtttcacaac aacttgctga ttacctatta
    43141 tttcatcaac aaaaatgcat actgctttat gctcattttc aataattatc atattaccat
    43201 tcatcagatt ggttactttt gtttgaatat tatatatttt atgtaacctt ataatgggaa
    43261 tacatgctcc atatattgta attaactctg tcttatttcc ttctgtaatt atttgattta
    43321 agtctgtaag tttaaatgtt cttcttatgg atgatattgg taaagtaaat agtgaatctc
    43381 caactttaaa cttcataccc tctactatag aaagagttaa tggtattttt atagtaaagg
    43441 ttgtcccttc atcttgttta ctttttactt caattgaacc tccaactttt tcaatacttt
    43501 tacgaacaat gtccatgcct actcctcttc cagaaaattc agttatagat tcttttgtag
    43561 aaaagcctgg agtcataatc aaagaattga cctcgtcatc agtatactca ttactttgat
    43621 gtttaagaag accttgttcc ttagcttttg ccaaaatctt atctctcata atgcctctac
    43681 catcatctga aatacttatc acagcttctc cgcctagatt ttcagcagat aaaactatct
    43741 ttcccacttc ttctttacct ttcgctatac gctcctcaat aggctctatt gcatggtcca
    43801 tagcatttct tataaggtgc ataaaaggtt ctgctaaatt atcaattaca ctcttatcta
    43861 cctctgtctc tgctcctata gttattaact ctacattttt atctaatttt ttattcatat
    43921 ctcgtacaat tctatgcatt ttttgaaata caccagcaat ttgaatcata cgcatagaca
    43981 ttactatatc ttgcaattca tctgtaagct tgtgtaattg tcttgttgcc ttattgaaat
    44041 tatctatatc aacacctgta gaatttacgc tatttacaac cattgattcc ataattacaa
    44101 tttctccaac tatattttga agtaagtcca atttttctaa atttacactc ataatactct
    44161 gcttacttat tggtaaatgt tcattttctt cacttagact atctttttcc atatcagttt
    44221 tttcatttaa aacatcttct tgtatctcat cttctgtata gttattttga taacttttac
    44281 ttactttatt attatctttt aatttatttt ctactgcatc attgtttgat gtatttttca
    44341 tttctatttc ttttttacta atatttatgt attcttttac atagagatta tcttctatag
    44401 ttttataaac tattgcctta tcctctttag ctacaaattg tatataaaac ccataacttt
    44461 taatatactc tgtagactct gcatttgttt ctacatcttt tggataaaaa tctataaatt
    44521 cacagtaatc tctaagttca aatacaagca tgaaagctct cacattttcc atcatgcaac
    44581 cttgttcaaa gattattttt actacatcag agtcttcttt gtattcaaaa tttgaatcga
    44641 ttacttcttt ttcgtcttta ctttctaaat ttgcattatc ctttgatttc atagaacttg
    44701 ataattccaa agtactcaca tactcttcaa ttccttcaat aaaattagag taatcagaag
    44761 atatacaacc tcttgattgt aactcatcta tatctcttct tataaaatct aatgattgaa
    44821 agactaaatc aaataatagg tcattgtcta aaacttctat tttaccttca cgtatagcat
    44881 aaaacaagtc ttctacacta tgtgctaaag cagatatatt gtctaacccc atcatagatg
    44941 ctgaaccttt tatcgtatgc attattctaa aagtttcatt aatgttgtta tgagtaaata
    45001 ctttctcttt ttctatttga atcataattt catctaattg ttcgagtaat aaacttgtct
    45061 caaatataaa catatctaac atttgtctca tactcggttc aatataattc acatttatca
    45121 cctacgacct acaaataatt ttctattata tatgcatctc ataatttaaa tccttgatga
    45181 ttctcttatc tcactatatg tacctcataa tttaaataca caattattct cttacttcaa
    45241 tatatataca tctcataatt taaatacatg ataattctct tatttcaata tatatacatc
    45301 tcataattta aatacatgat aattctctta cttcaatatt cacctcataa tttaaaccca
    45361 tgatgattct cttacttcac tttcctttag atgaaatttt tctactaatt cttttaaaat
    45421 ttgagcttga gcagacatct cttctgaggc tgctgcactt tcctctgctg ttgctgagtt
    45481 catttggact actatggaaa tttgctctat tccagataaa atctgtgtaa ttgctacttc
    45541 ttgactatgt acagccgaag aaataccatt aatataatta gtagtttttt ctacaccatt
    45601 tactaccaag tccaatgcat ttgcagtctc tttagcaatt ctgacaccat tttctactgt
    45661 tttaatagct tcttctatga gaatagctgt attttgagct gcttttgcac ttttaccagc
    45721 caaagaccta acttcatctg ctacaactgc aaatcctcta ccatattgac ctgctcttgc
    45781 tgcttcaaca gctgcattta atgcaagaat attagtttgg aatgctatct catctattgt
    45841 tttgattatt tttgatattt cactcgattt ttcacttatt tcacccattg atgtcatcat
    45901 atcatccata catctacttc caaaaccaac ttgtgatgca gattggtcag atacctcaca
    45961 tgcaagacta gcactatctg cacacttctt aattccatct gatatctcat taatcgtgga
    46021 agctaattct tctactgaac tagcttgttc agtagcaccc tgtgaaagag cttgtgcacc
    46081 tgctgcaact tgttctgaag cacttgcaac ttgctctgct gataaattta tttcagaaaa
    46141 atcaccatta aaagagttta ttatactttc taaagatatt ttaattgatt taaaatcacc
    46201 tataaattct tggtctatct ctactcttaa atctcctttt gcaatactag ttaatgtact
    46261 gtctataagg tctatgtaat ttaaaagcat actttgcatc tttttgacag aactcactaa
    46321 tagccctatt tcagatttac tttcttcaac gtctatactt gttcttaagt ccccagatgc
    46381 aattcttgaa aagtgttttt ctactttatt aataggaatc actattttta attttattac
    46441 tgtatattct attattgtca atattattaa aagggaaagt ataaatattg ttcttaatat
    46501 tagactttgt aattcttttt ttacctctac aattttggaa tttgaacggg taactatatc
    46561 ctcttgaaat tcatttatta aagaatccat tttctcttgg gagtttatat agtcactacc
    46621 aaaaattata ctttgagctt tactcttatc attactttcg actgccttta ttgcactttg
    46681 ttctgttggt actaaagaat ctgatatttc ttttgcttct aagattttat ttgactcgct
    46741 ctttgtaaga ccaatttttt ctaatttgtt taaagctgat tctcttgttt ttccgtataa
    46801 ttctttttca taaatatctt tattcttttt ttctcctgta aatacatatc ttcttaatac
    46861 atcacttatc tctctagttg tatcttttat atactgactt tgagtcctca aattattcat
    46921 atcattaata cttttttcta ctttttcaaa tgctttataa gatgaaatag aagaaaaaat
    46981 ccctgctaga acagccaata ctataataat cagcaataca attgctaatt tagaaagctt
    47041 tacttctctt ttcatatctg tactccttca taaagtaagt ttatatcttt tttaatcctt
    47101 tcattgtact cactacgcta caatcttttc ctaagttttt accctcatac attgctttat
    47161 ctgctaagga tatgttttct tctaatgata tatttgagtt tacttctact actcctaatg
    47221 taatagtgca ctttatttta tgttctttat agttgaaatc aaactccttt atctctttac
    47281 ggattctttc tgaaagtatc ttagcacctt ctattggagt atttgtgagc attatcaaaa
    47341 actcttctcc accccatctt gatacagtat caatttgtct acaattcttt tttattatac
    47401 ttgatattac tgttaacacg aaatcccctg cttcatgccc ataagtatca ttgataacct
    47461 taaagtcatc aatatcacca aggataaggg atattgatgt ttcactatat ttattaattc
    47521 ttctttgctc cataagctga ttaattatat gtcgcctatt gtaaagacct gtaagataat
    47581 ctcttacaac gagtctattc aatttttcaa gagaagattc cagttcatta tttatctttt
    47641 ttaatttatc ttgagatttc tttacttcaa gatgagctat aacacgagct tttaattctg
    47701 tcatactaaa aggtttcacc atatagtcaa ttgctcctac accaaatcct tttactatgt
    47761 ctgtatctgt attttttgaa gttataaaaa ttataggtat atctgctgta cgagcattag
    47821 attttacttc cttacaaaac tcaaaaccag agttatctgg taaaattata tcaagcaaaa
    47881 taatgtctgg tataaattct tcaataaatt cttttgcctc tattgcatta aatgcttttt
    47941 taaccttaaa attatctttt gtaagtgttt tatctattaa gtcacacatc agaacactat
    48001 catcaacgac taaaattgtt ccctgttgct tatttttcat atacaaacct ctcttaaaca
    48061 taatatattc aaattttaat gatttaaaat ttttatactt aataattata ccactatatt
    48121 cttaaatgtt ctcatcacaa tctttttata ataacactcc aactacatcc ctatgttaca
    48181 tgatagtgca atcaaaccca ttgattaatt catctattaa aacttcatct ttatctgtca
    48241 aaagtttctc taaatctagt aaagtaatta ttttaccttc taattctcca actccactta
    48301 caaaataatc cgaatattct atagtaactc ttggtggagg tgatatttta cttttttcta
    48361 agcttactat atcatcaact ctatcaacca caaatccaac taccaagtcc tttatttcag
    48421 ttactactat atacatgcaa tttgtagtca ttaattcatc ttttttaagt ctcaaacgca
    48481 tgtctattat cggaatgatt tttcctctta gatttattat gcctttagaa tatgtaggta
    48541 gttctggaat aaatataatt tcttgatttc cagtaatttc tattaccttt tcaagttcaa
    48601 ccccaaagtc ttgctcatct actctaaact ttagatactt aattccttca tcaattagtc
    48661 cataatctaa atatgggtca tctggaaatc tcatttaata cctccctcct aaaatacact
    48721 ttctatgaaa tatatattat aatttcttct tacctttatt tattgaatct acaagtaatt
    48781 cacccgtctc agtgtaaaaa cgtattgttc taccataatt catacctgtg tcctcagcta
    48841 atattggaat atttaatcct aaaagagttt tttttactgc ctcaacattc ctatgtccta
    48901 tacttgcaat tactgaattg ctacttccta aaaacatctg agcacctcca gcaatttttg
    48961 cttttatata tattctattt gcacctatat tctccataga tttaatgagc tcatttattc
    49021 ctgtgtcagc aaatttacac ttattttcaa tgttaatagc atctttacta tatggcaaca
    49081 taacgtgtaa cattccacca attccagcta ctttatctat caaacatact cctacacaag
    49141 aaccaagtgc ataagtaact aaaacatttg gtctataggc tatcttcata tctgcaatgt
    49201 tcactactat ctctctattc atacttctaa tcccaacctc acgagtattt ctctaagtga
    49261 gctcatttct ggaatcatca aaatgtgact agtaaaataa ttatcactca ttttaaattt
    49321 attttctata aataagactt tgtcaccaac atctgaaaat cttatcatag gtacatttag
    49381 aacagctcca accatatcaa tacatatatc tggaattgaa acacttatag tcatgtttaa
    49441 catagatgca atagcattta catatgaact tgccataata tttcctattt ccttaattgc
    49501 tgaaatttca attgcctcaa tctcatctaa aaaagcttct tctttattaa aaagagaact
    49561 tagcataagt tgagttattg attcatccaa caaaaacata aacattccat tgatatcacc
    49621 tttcatatcg agtaaaatac atactacttt gttttctgga ccaccaagca aatttgtcgc
    49681 ttcattatat tcaagtatct tgaccatagg tattaatact tctacattgc tatttatcat
    49741 ggatgcaagt gcagtaattg cattcccaga gcctatatta ccaatttctc taagagcatc
    49801 tatatgaaca tcatcaagtt ctaaataatt tataatataa acccctccct ttattattgc
    49861 atttatttgc ttttcattgt attagtgtat atttacactt ttattttaaa gcattatcta
    49921 caactttaac taatgtttct gttttgaagg gcttcactat aaaatctaac gctccaagtt
    49981 ttatggcttc tacaatcata ttctcttgtc ctattgcaga gcacattaca accttagcac
    50041 ttgaatccaa cttttttatt tctcttagca cctctaaacc atcttttttt ggcattgtaa
    50101 tatcaagcaa catcaaatca ggcttttcat atttaaatat attacacgct tcttctccat
    50161 catttgcttc tattaaatta ttatagccac ttttactcag agcttcttta attatcattc
    50221 tcataaaaat agcatcatct actatcaaaa ttttattttc cataaaacta actcctccat
    50281 atgtacaaca ataaacgctt ttatcttcag ttattgtcta tactatctga ctatttttcg
    50341 ctacacaata aatacatttc attgataaat aaaagtaatt atttatgcca tattatactt
    50401 aattctctta acataatttg tcaacaaaaa aacttatata tctattgtaa tatatgtttg
    50461 gcgttttgac gagttattaa aaatataatt gtataaaaca taaaaagtta tcaaaagtaa
    50521 ttaaattata ataaaacaac tttatacctt ttttatgttt gtataaaaaa atttttcaga
    50581 ttcattaaaa tcatttctgt agattcatat aggatttgac tttctcaata tctaatagat
    50641 agttgtttat ttacttaatt atatttctct atatgtatta tcgattaaat tagatttagc
    50701 tttatacatt attttttata tttttcaaat tgtatactca taaatccaga agtattctaa
    50761 tgtgtttata atactttttt tcatatttta aattaaataa caaatcatta tatttacaag
    50821 tatcatttat caataaacaa tattaaatga agaatatgtc tgaagattca aagtgatact
    50881 gataatatta attactaaaa aagcagtaaa tcttttacag atttactgct tatagttata
    50941 aataataaaa ctatctataa taaatgtact atactcaata cttcattcaa tatagtaata
    51001 acaaatctac tttattccta tataaatatg aacttcttgt ttctttgtat caaaagtatt
    51061 acattgatac tcttcaaaat cacttatata agtcctatca aaatccattt gccaaaactc
    51121 aaaccaaaaa tctcctactt ctttttgacc tcctactata acaaactttg catacttacc
    51181 tgctggtata actttactaa taatactttc tccaacagta ttattagtgt ttaattcttc
    51241 taaattcttc tctttatcag aattactatt tacttcacaa catgcaacaa agctatacgg
    51301 acttgtaaaa tcaccctggt aatctgtata tagacctatt gtcttgtcat tctttttacc
    51361 ctttatagca ttatatatcc ctttgcccat aaattttttc cacaattcac ctatatcatt
    51421 taccgcttgt ccatctttat ttgtagtctc cttcgatatt cctataacta ttttctcatt
    51481 tatctctact atttcataat tcatacttta tacctcactt tgtttttatt aatatatttt
    51541 attttgaact aattcaatta taatttataa tatacgacac ctttatgtca tatttttaat
    51601 ataattaaac tcagtgagat attatatact aaatgcaaga tattatatat ttaataaaat
    51661 ctttaatcta gcattatatt ataatgaata aattttagac aactccctta gtctagttat
    51721 tacattatcc ctaacatctt taggttcaag tacttcaaca ttgtcaccaa atgataaaat
    51781 atatccataa atccattcac caataggaaa ttctgtaatt acatcatatg tatcatcttc
    51841 atttttagtt attaaatcct tattaaaatc atcaaatact ctaaaaagca tcttttcttt
    51901 aaacctcaat ttcaacgtta tagtattttc cttaattact ttagaatcat tcaaacatac
    51961 ttcttctatt atctttcttg aaaatacttc atcctttata gataaattcc ttatcctact
    52021 aattctaaat atcctaaaat catcttttag tttacaaaat ccatacaagt accaagtctt
    52081 actcttatac ataagcttca gtggttcgac agttctgttt gtctggcttg acaaactatt
    52141 tacataatta aaatttatag tattacgcct taaaatagct tcttttattt ttgtaaattt
    52201 atcatcttca ttaaaattac ttccccattc agtaaagtct atttcaatcc agtttgaaaa
    52261 attctgctct ccaaatattg agccaatttt atttactact gaatttattt ctggatactt
    52321 agtcgcctgt aatgttttca gtgccactat taaactctct ttatcttttt ttgacaatat
    52381 agccttgtct atagaatatt cttctattaa agatatccct cctcctttgc ctttcgtcat
    52441 atatactggt actcccgaca tagacagagt ttctatatct ctatatatgg ttcttgtaga
    52501 aacttcaaat ctatctgcca actctttagc tgttactaat tttttattta atagaattac
    52561 tattatttct gttaatctat ttattttcat tcattaccac ctactaaaat tattaaattt
    52621 atttaatcta aattaatcat actaattttt aaataaataa aatcttagaa tcttatctaa
    52681 attattctta catgatttta tttactttct attatctttc tcactttgga tattatcatc
    52741 ttaatttatt tattactata tcaatttact tattatcatc tcatttgtta tcgtcttact
    52801 taatttactt atttctatat caattcactt attattacct cacttgttat catcttactt
    52861 aatttactta tttctatatc aatttactta ttatcatctc atttgttatc gtcttactta
    52921 atttacttat tgctatgtca attcatttat tattatctca cttgttatcg tcttatttaa
    52981 tttacttatt tctatatcaa ttcacttatt atcatcttaa ttcatttgat tattatcatt
    53041 tactttattc aatttctata tcaaaaatta gtatttgtac ttaatactta aaattaattt
    53101 aaagtcttta catattattt attttttatt gtaatattaa tttccatact ttgctaagct
    53161 acttccactc atttatttta aaatttcaat ttactatttc tcaattctat attattattt
    53221 ttcaatttta tattactaat tttaaagctt tttagctatt attcctacaa tatttaaaca
    53281 attttttaat aataaagcta tattacaata tattcaaaat atgtaatatt ttagatattt
    53341 ttcgttgaca atgttaaatt tttgttttat ccttaattaa ggacatgtta ttgacatttc
    53401 tgaaacttat ttgaaatagt tttttcattt tatttaggaa aacgtattat acctaagaaa
    53461 acgtattttc tcatttcaga taagtaaaat atttattctt ttggggagga aatcatggaa
    53521 attaataaaa aacattttat tcaagtattt ttaacttgct taatcttggt agtaatatgt
    53581 gaatttatag ggcaaagaac tatagctatt ggaaaagcct ctatagtact gttccctatg
    53641 ctttatgcag ttataattgg acttataata acaccagatt tattaggtaa aaaaattaaa
    53701 gcacttaaaa aagtggttgg agaaaaagaa ataaatattg ctggtgaagt tgttggaatt
    53761 gcactagtaa tgcttggtat aaaatatggt actacagttg gaccaaacat tgacaaaata
    53821 attcaagctg gaccagcatt tatcgctcaa gaatttggtc atatacttgc accaatcgtt
    53881 gcattacctc ttgcattaat gtttggaatg aaaagagaag ctgttggagc tactgcaagt
    53941 ataagtcgtg aaccttcttt aggagttata tcagaaaaat atgggataaa ctcaccagaa
    54001 ggtagtggtg tacttggtac atatttaatg ggaacagttc ttggtactat atactttagt
    54061 gtactaggtt caatatctat ttatagtggt cttcacccat atgctttggg aatggcctgt
    54121 ggtgttggta gtggaagtat gatgacagca gctgctggtt cattatctac catggtacca
    54181 cctgaaatgg cagatacaat acttgcctat gctgcaacaa gcaatatgat gtctagtata
    54241 acaggaatta tattcttagt ttttgtatcc ttaccattta ctaattttat gtataagatt
    54301 ttagaaccta aattttcgag aaataaaaaa actaaagaaa ttaaatctac agaaggagag
    54361 tgctaataaa tgtttgctaa tttaaattta aagaaaagtt catatcaatt attggttatg
    54421 tctgttattg gagttataat tcttatagga caaagaatta gcgttggtac atctatagtt
    54481 acagctcttc ctggtatggt gatgctaata ttagctgcta tggcagctat gattataaaa
    54541 gatttatttc ctaaatcaat ctttccagct tttggatttg caactataat cggcctttta
    54601 cttagtatgc catatagtcc aacatctgaa gtattcttaa ctaacactaa taatattaac
    54661 tttatggcta taactactcc actactagct tttgcaggaa tttctgttgg aaacaaaata
    54721 gaagctttaa aagaaatgtc atggaaaata gttataattt cacttattgt atttacaact
    54781 atattctttg catgtgcgag tatagctcat atagttttaa gtattcaagg taaaatatag
    54841 gaggaattta ttatgaataa agaacaatta aaatctctag taataaaaac tatagatgaa
    54901 aatagagaaa aaatattaaa aataggtcaa ggaatataca aaaatccaga atatggatat
    54961 aaagaattta aaactacaga agctgtttca aacttttttg agcaagaatt aaatttatct
    55021 gtagaaaaaa atatagcagt tacaggatgc aaagcagatg caaattctag caagaaaggt
    55081 cctcatatat ctatacttgg agaattggat ggtatatcat gtaaagaaca taaagatgct
    55141 aatgaaatag gtgcttctca tacttgtgga cataatattc aaatagctgg aatgttaggt
    55201 gctgctgttg gtttagtaaa aagtggcgtt ttagaacatt tagatggtaa agtatctttt
    55261 atggctactc ctgctgaaga gtttattgag ttggaataca gagagcaatt aaaaaaagaa
    55321 ggaaaaataa cttattttgg tggaaaacaa gaattaataa aaagaggtta ttttgatgat
    55381 atagacatat ctatgatgtt tcatgcttct gatttaggag aagataaggc tctagttggt
    55441 cctgagagta atggatttgt tggaaaaaag gttaaattta taggtaaaga atctcatgct
    55501 ggttctgctc cacatgatgg aataaatgct ttaaatgcag caatgcttgc tataaacaac
    55561 gtgaatgcac ttagagaaac ttttaaagag tctgaaagag ttagattcca ccctatcatc
    55621 acaaaaggtg gagatatagt aaatgttgtt cctgctgatg ttcatatgga atcatatgtt
    55681 agagctagaa ctataaatgg tatgattgat gctaatgaaa gaataaataa ggctttgatg
    55741 gctggtggaa tggcagttgg agctgatgtt gagataactg aaattcctgg atatcttcca
    55801 atattacgat acaaaagtct tgataattta tttaaaaaca atcttgaaga tttaggtcta
    55861 aaaggaaaag ttattgatgg tggagatttt acaggctctt ttgattttgg agatgtgtct
    55921 catataatgc ctactcttca ccctatgatt ggtggtgtaa aaggagctct tcatacaaga
    55981 gattttgaaa tagttgatga agatttagct tacataattc ctgctaaagc aatggcactt
    56041 acagttgtag accttctttt tgataatgct aaggatgcta aagatatatt agataccttt
    56101 actccagtta tgactaaaga agaatatcta acattcttag aaaaacacga taaaacttta
    56161 taagtctatt tttataatag ggtggtatct actgtaaaag ttataccact ctttgttttt
    56221 gtaacagtat acttcttttc ttttagtttt tagattaaac taagatatat tatcctcctt
    56281 aaaactttta gagctacgtt gaaatatcag tagaactatg ctatattctt tctaattaat
    56341 ataaatttct attctaattc aaggtggtga aaaaatgttt aatgcaggaa atcgaattat
    56401 aaatacatgg ttgtacccta ttcaagatgg ctttgtcttg attgatactg gatatgaaaa
    56461 tggttttaaa catttcaaaa aacgaatttc taaattcaac ataaaaatca aagatattcg
    56521 atacatattt ctaactcatg ctcatgatga ccatgttggt tttctaaatc agattctaga
    56581 cgaatctcct aatacaaaga ttgttatgca tgataaagct ttagaaattc tatataaagg
    56641 tcaaaactct tttattggtg gttgtaccag ccgtactgcc tttctctttt gccaaattat
    56701 gaaactattt ggaaaaggtg agcatagttt ttctccactt aaacaagaat ttgaaaaaag
    56761 gtgtattctt gtgtctgaca agaatcgtaa agaaattgaa aaagaactaa atggaaaaat
    56821 tatagataca ccaggacata ctgctgattc aatttcacta ctaattgatg aaggtgtttt
    56881 attttgtgga gattctgcaa tgaatggatt tccaagtact cacaaaatta ccatatttgc
    56941 agaaagcaaa ctagaattta ttcaatcttg gaaaacaatc atcaatacaa aacctaaaat
    57001 gatttatcct ggacatggaa aaccatttga atacagtaaa ttagaacaaa atctaatcta
    57061 tgtaaataaa ataaatttat atcctctaca taattcaaaa gatattcaca ggtttttgta
    57121 gaaaatacaa agtaaattta tttgattaat taaaaaatct aacactttac tacgctactt
    57181 attttaataa cttaatcttc cactagcttt tcataaaatt ttgttttaaa attaatttta
    57241 aagcgcatat gttatttaaa tacattagat aaaattcgtc attaatctac ttaaagcata
    57301 tttaactaac accactcaac cagaaaattc agaaaactgc attgacacag gaaaaagttt
    57361 attttatact ttttttagga catattgtgg acacgtctta aatcgacaaa cagaatttaa
    57421 attgtaagat taaccacaat acttattttt taattatatt tttaaacatt agggaggata
    57481 ttatgaatga taacgtagtt aagaagaaaa acttttttga caggacatta aacagaatag
    57541 aaactgttgg taataaacta ccagacccag ttacaatatt tttagggtta tgtgtacttt
    57601 tacttatact atctagtcta gttggttcta tgggtatatc agtagttcat cctggtactg
    57661 gagaaactat aaccgctgta aatttattga ctgtagaaca gcttcaaatt cttttaggaa
    57721 acatagtcag caatttccaa gggtttgctc ctttgggtct agttctagta actatgatag
    57781 gtgctggtgt atgtgacaaa acaggactta tgacagcaac aatcaaagca tcagtaagca
    57841 agattcctga aacaagagta actcttgttg taatgactat aggtatgctt gccaatatag
    57901 cttcagatgc aggtacaata ttatttccac cattagctgc acttgtatat cttggtgttg
    57961 gtagacatcc acttataggt ttattttctg gatatgcagc agtctgtcta ggatttgccg
    58021 caaacattat gataagtgtc aatgatatac ttgctgcaag ttttacagtt ccagcagctc
    58081 aaatgattga ttctggattt caagccaatg caacaatgaa cttatttttt atgatagcat
    58141 caacttttgt tctaatactt ttggcaacat gggtaacaga aaaaattgta gctcctagat
    58201 ttggtaaata tgaaggaaat gcagaactag atgttgatgg aagtcttagt gatgtagaaa
    58261 agaaagggct taaaaaagct agtataagta tattaatata tgtggctatt atagtagctc
    58321 ttagtgtaat cggtaaaaaa ccattcttag ctgacccaga aacaggagat ttactatcaa
    58381 acaatgctcc tcttatgaaa ggtatggtac ctataatagc actagcattt ttcatacctg
    58441 gtgttgtata cggaaaaact ataggtaaaa taaagaagga taaggatgta gtctctataa
    58501 tggctagtgc aatgagtgat atgggtggat atataatact agcatttgct gcttcacaat
    58561 ttttgcaatt attcacaaat agtaatttag gtctgatact agctgtaaag ggcggagaat
    58621 ttttaaaatc agcaggaata aatggacctt tattgttgat aggatttatt atattatcat
    58681 gttttataaa tttatttata ggtagtgcat ctgcaaagtg ggctattctt gcacctatat
    58741 ttgtaccaat gtttatgatg gttggataca atccagcact tactcagatg gcttatcgta
    58801 taggtgattg tataacaaat ccacttagcc cattatttcc gtacttccct attatactag
    58861 cattcacaag aaaatacgat aaagatgctg gtatgggtac tgtaattgct aatatgatac
    58921 catactctat atcattctta atagtgtgga ctatactatt aatattgttt atggtattta
    58981 atattccact aggacctgga atacttcctt catatagcat gtaatttatt taaatctttc
    59041 taaaaggaga aataatttat gttagataaa aatagactta taaataattt tatggatatg
    59101 gttagaattg atagcccatc taatcaagaa ttagaaatgt ctaagtggtt ggtaaactac
    59161 ttaaaagaaa gaaatataga cgccattata gatgatgcag gtgaaaaata tggtggtaat
    59221 acaggtaatg tcattgccta tataaaaggt gaagaaggaa gtagaccact ttgtttatgt
    59281 gcacacatgg accaagttca accttgttta ggtgtaaaac caatactaga tggtaatgta
    59341 gttagaagtg atggaactac tacacttggt gcagatgata aagctggaat agctgctata
    59401 cttgaagctt tagaacatgt tattacagaa aaaataccac atagagatat ttatctttgt
    59461 tttacaatct gtgaggaagc cggtatgcat ggtgtcaaaa actttaatcc tgacaacttg
    59521 ccttgtaaag acatggtgat tttagattct ggtggagcta ttggttcgat agcatacaaa
    59581 gctcctgctc aacaatctat aaaaatctct tttcatggaa agaaagcaca tgctggtata
    59641 gaacctgaaa aagggttaaa tgctatatta gtagcctctc atgcaataag caacatgcac
    59701 ataggcagaa tagattcatt gactacttca aatataggta aaatagaagg tggtggagct
    59761 acaaacatag taactgataa ggttacattg actgctgaaa ttaggtctca tatacctgaa
    59821 actttagagt atgagcttaa tcatatggaa aaatgttgta aggatgctgc atctaaattt
    59881 aatactactt atacttttga acatagtatg tcttacccta gttttgaact tagtagagat
    59941 agtcatgttt tcaagttaag tgaagaagca ataagacaag ttggtgttgt tccaaatcca
    60001 atggtaattg gtggtggaag tgacgcaaat atattagcta atcttggata taattgtgct
    60061 atattaagtc taggtatgta tgatgtacac acagttaatg aatatgtaaa tatagacgaa
    60121 ttatatgata ctgccaaaat agtttatcat atgattaaat tataaatatc ataatataac
    60181 tctttaaatt ataaaaataa aaacctatgt atcttcaact ttataagtta tatattaaag
    60241 ttgttttaca taggttttag tattttatat aataatttgt tttaacattt ttcataataa
    60301 gcttcaatat tttgcataat aatttgttct agcattttta ataacaagct ttagtatttt
    60361 atattaataa tttattctag tattttacat aatcatttgt tctaattttt ttcataataa
    60421 gctttaatac tttatatagt aatttgttgt agaattttat acaatataat gatttataca
    60481 gttttataaa atatcttaaa acagtatttc agtaatattt tttggtcttc ctccaccaat
    60541 actagtctct tttgcacata ttcgacctaa acctgcattt actattttat ttaatatacg
    60601 tcttgcactt ctttcactta catttagata ctctgctaac tcttttgaat cataaatctt
    60661 actttttcta ttctcattta tagcaataat ttttgcaact gactcacaac tcattccagt
    60721 ctttttagat atttcgatta ccttttcatc agatagcatc aaagaataat ttaattcatt
    60781 ttcggaagct aaaggtcctc ttatgttttc atcttcatct accaaatata taccaatttc
    60841 ttttgaatct atggagtgtc ttaatgcttt atgtgcatta ttttcagcct gatacgcagt
    60901 tactccagta ccaattccta tactaagaga aaagcccatg tcttttatct ctctctttag
    60961 attgaatagc ttatcataat tatactcatt atcagctacc cctttatgaa caaatacaac
    61021 ataatcattt cttccaagta aaaataagga accttgaata cttcttacat agtttactac
    61081 aagcttatcc atatctgatt tttttatcat gtcagaataa tagttatcta tcttctcttt
    61141 ataatctatt aaactcaata tttcaacagc aatttgagaa aatcttgcct tacttagagc
    61201 atattcactc tttaccttgt cataactttc tttgattaat tgtatagtag cttgtaaacg
    61261 aaatacagga tagcctcttt cttttaattc attatatact gcaccaaaac cagttaacat
    61321 tatatccgtc ttattttctt caaataattt tatatgccat tctacaagtt cagactctcc
    61381 tacttccaaa gaaaatggat tacaaaatac actcgttgct ttaatatcaa attctttaag
    61441 tgcatcttcc acaacatatc tatcaactac atctatactg aatttatcta attttatatt
    61501 tgcacttttt attgcccaaa atgccttcat tatactcgtc ccacttctcg gtacaaatga
    61561 atgtggctta cttatctcat acttagattt tataaattca taaactgcac atcctgtaaa
    61621 tataatagca tcacattcgt cttcacattt agaaataact tctattgtat ctactacttt
    61681 ttctcttaca tatattttag tctttaaatc actgtctatc tgttttaaat catcctttat
    61741 cttaaaccca gagtctgaag gacctataat acccactatc ataaacttcg ctcctaatcc
    61801 ataaatatta tactttttaa aaattatatc ctcactattt tctacttcta tttaattatt
    61861 ttgaactact ccatcctacc tacatttaga atggaataat tagcttattt ttgataaagt
    61921 aacttctcaa aatcagaact tggaataggc ttacaataat aataaccttg aattaccaca
    61981 caatctaaac tcactaaaaa ttcagcttgt tcttttgtct ctacaccttc tgccactaca
    62041 tccatatcta aatcttttgc catggctata gtgtgcttta aaactatttt ttctctgtct
    62101 gtgattcctt cctcattaaa gaaaccacaa tctatcttta atgtatcaat attaatattt
    62161 tttaacatat ttaatgaaga atatgctgaa ccaaagtcat ccatatttaa aacaatacca
    62221 tccattctaa ggtcggctat tatcttagta aataatttta tatttttaat aaataaactt
    62281 tctgtaagct ctaattctaa aaaagatttt ggtatactat atttatttat taaacttaaa
    62341 acaatttctt taaaatgtgg attatgcaaa tgtaatcttg atacatttac agagatagga
    62401 actggctctt cacctaaatc tatccatttt ctaatcaaag aacatacttg ttcccataca
    62461 tacatatcta atctgataat aaacccattc ttctcaaata gtgaaataaa tttaattgga
    62521 ggtattatac ctttttctgg atgaacccat ctaaccaatg cttctgcacc aacaatttta
    62581 tttgcattaa gattatactt aggttgtaaa tatacatgga attgcccatt ttcaagtgcg
    62641 tattccattt ccctttccat ctgtttttct tcaagtgcat catttcttat agtatcatca
    62701 taaaatatat agttgttgat actttgtttc tttacttttt tgtgagcata tcctgccatt
    62761 tcacacgcat ttgtaattgg aatatgatta tccaaaattc tataaacccc aaaacatggt
    62821 actaacatat attcaaattt ttttctattt aagctatttt taatcttata aattacttta
    62881 tgaatatctc tatcagagta actctcataa aaaatgcaaa atacatctgc atacatacga
    62941 caacatatac cattagaacc aatgtttttt tgaatcaact ctgcaacata tattaaagct
    63001 ttatctcctc ctctaactcc ataaatgtca ttaattacct tgaacttatc tatatccatc
    63061 attataattg cataacgctt gtcaggatat cttttaagca ttaaacttat ttgttcacaa
    63121 aaactttcat aattgtatag tcctgttaat gtatcttttt ggtttttatg tagttgcata
    63181 agataatcta tattttttat agcaactaaa atatgtgttt gtttctcacc taacttattt
    63241 ttactactaa tctttaacat ataaattaat gcccaatagt actttttatc tattcctaaa
    63301 cgtctatact caaaatttag ttctaaatcg tcttgcttgc tattattaag actatcaaga
    63361 gattttagaa ggtggttatc attaaataat attatatcat caggatgaat taaatatttt
    63421 tttatgtatg aaaaagtttt ttcaagattt tcctttatag gaaaactaaa aatattgctc
    63481 tctgattgat aaattggagt aattaaattc gtatcaatgc aaatagataa aattatatca
    63541 tatgtattat aagctaattt ttcataagct ttagaatacc cttttatcat taaatcatca
    63601 aaataaacct tatcaactaa ctctttatgt ttaaataaaa tattattcgt cgaatcagct
    63661 aagtcactaa ttactttcat ttcattattt ctaagtttgt tcttattata atacctcttc
    63721 ttatcttggt acattctctc atcagcaact ttaattagct tatgtatatc aatattatcg
    63781 tcactccaaa tactgcccac tgctacacta tatatcatct catccttaaa ttcttcactt
    63841 aaccaactca ctaattcttc aaatagttgt tttgatatat cttcacataa tacaacaaac
    63901 tcatctcctc caatacggta aacattataa gaatgaaagt atttttttat tattacacat
    63961 gctgacacaa tagcagaatc gccattagca tgtccaaagt tatcattaat atcttttaac
    64021 ccatttatat ctacaaatac aactcctatg gatttaactt ttcctttttg aaaattatct
    64081 atatacttaa tatatttatt tctattatat gcaccagtca agaagtcata gtagctcata
    64141 taatttaatt tattttctac ttttcttttt ttcatctcag ttaataaaaa ataagatagg
    64201 gatgtcaaaa gagaggtatc tccaacagct ttatgtggat tatcaactcc aataaaccct
    64261 actactttat catcttgttt tagtgaagat gtcattaaac tttcaatttt ttgaggtttt
    64321 aaaatttcat attctattgg ctgttcttct tttatatttt ctatatcatc aattattaca
    64381 aaaccttttt gttcgaataa taaaaaccaa ttctttaatt cattaatatc aagatttgtt
    64441 agtgcctgtt tttgttcatc aacaccttca ttgcaccatt catatgtatt agttactttt
    64501 gttctatctt ctgatacttc aaaaacatat gctctatcag ctttatgaaa tttaccaata
    64561 ttggttaata caagatttac agcatcagaa aaattagatt ctgttataag atgtttaata
    64621 catccaacta ataactcctt tatctctaat ttttctgcta ttgacttact tatatcttcc
    64681 ttttctgtaa tatcaagagc tatttctaat cgtgcatgtt tgccctccca tttaattaac
    64741 ttgtccttta atataaaatg tctatttgtt cgagtattcg tattttccca tatgtaatat
    64801 tcatcttctt ttaaaataga gtttgtacaa aattcgcagg gagaatcttt cccctgtaat
    64861 gctttataac attttaaatg ggatatatca ttaagtccca ttgccttttt cccaatttca
    64921 ttaatatata aaagttcata tgtatctaaa gaagaaacat atacaatttc atccaactca
    64981 tctattaaat ttattacatc catttatata tatctccttt tattcatttt ccagattgaa
    65041 tttttaaagc ttttaaaatt ttgctatata tttggcaatg atattatact tctttatgtt
    65101 ttaaaatagt accttaatga ggttctattt tttagtaaat agaattatta ttgtgtcaat
    65161 cgtacaatag agttttgtaa tacttaattt aatatattta taattaacta tcttattatg
    65221 tatttacaat taattctcat ataaatgaca aatctccacc tcaattaagt attccaatca
    65281 tatatgagga taatacttca ttcatttaaa aatataaaac tattatatat taaaatttct
    65341 tcaataataa attatttaga ttgttacttt gatatattat ataatatcac aaaggctgat
    65401 ataaaaagac aaaaagggca atttttttat atcaaaaaaa atgttaaatt gatattatcc
    65461 atatataaat tgatattatc catataggtg ctatatttaa caattctact tacttccctt
    65521 acacttatta gcatatagtt attaagagtt tgttgcaaaa caacacttag cacactttaa
    65581 aaagtgtggt ctaataagta atcaacgaag aagacataat aaagcataaa ataggatata
    65641 cttttccacg tcatcaaaat tgaggatttt attattaata tatgataaaa tatgcttaat
    65701 aaattaaatt ttaatgggag aatagttatg aaaatattta gtttcttgtt gtttgtagtg
    65761 ggaatatgtt tttgtattgc tggatttatg gggcattcct cagctttttc tggtgcaaca
    65821 ttgagttttt ttgcaactac aatgatagct gtaagacaag tcaaaactaa gaaaaaatga
    65881 aataggcaaa tcctaacttt ataagttaat attacataag taattagtct aaaattagat
    65941 actttttcta ttacaaaaat acatcatcca ctttgtattc tcttaaaaaa aataaaatag
    66001 ctatgtaaat tcaagattag atagactaac tctctatcca tttcattgta aaatatacat
    66061 agctatttgt attcgattta aaatacttag attttcttac ataacaaata atttctatct
    66121 gattattact catgttcatt tattaaccac tcagcaacta atcatttagt atctattcat
    66181 ccgccatacg gtaacctacc ccaacctcag tcaaaatata ttctggctga gcaggattct
    66241 tttcaatttt ccttcttata cttgccataa aaactctaag cgactgattt tcactttcat
    66301 agtaatttcc ccaaacttta tctattataa acttatgggt taagactctt cctgaatact
    66361 tagacatcaa ctcaataatc ttatattcga ttggagttaa atgtatttct tcaccattaa
    66421 ttgacacaat atgattttca taatctatct ttaggttttt aacttcaaaa gactttggag
    66481 catcattatt tatattatcc tgttgagctt tatttctaag agccacacgt actctagcca
    66541 aaagctctgt tacactaaaa ggtttagtta aataatcgtc tgctcctcca tctaaagctt
    66601 ccaccttatc tctctcatgc tctctcgctg aaacaacaat tattttatta gtctttgcac
    66661 atgccctaac tcgtcctatt acctcaatcc catcaatatc tggtagtcct aaatccaaaa
    66721 ttatcaaatc tgggtcaaga gaatgtataa gtgatatagc tgttcctcca ttatctgtct
    66781 ctacacacct ataattttga gtttcaagag atacttttat aaatttacaa attggcttat
    66841 catcttctac aattagaact aatggcttta tttccacttg tctttcccct cctcatcata
    66901 cataggtatg ctaaatctaa atgtagctcc tccagaagga ttattaaagg cgacaatttc
    66961 tccaccatga gcttctacta tagatttaca tatctccaat ccaagaccaa tacctcttct
    67021 ttcaagattt ctagacttat tcttagtata aaacctatta aaaatattcc cgatatcttc
    67081 atttggtatt ccttttccat tatctgcaac ttcaaatagt acttgatttg actctttttt
    67141 tacagttagt tctatcttag aatttttagg tgtatgcctt atagcattat caattagatt
    67201 tactaacact tgttctatta ataatacatc cacatgaacg attattatct catctggaat
    67261 atttgttttt atgtctctac tattggcaaa ctttttgact cttaatatag attcagctat
    67321 tatttcatca acaacctctg gtcgtttttc aatatccaat tttccttcat ctattctggt
    67381 catactaagt atattctcaa cagaatgagt tagccaactc gtatcttcat atatattttt
    67441 caataattct actctagttt ctttatctat cacatcatca ttttcaagta ttgttgaaga
    67501 tgaacccaat atacttgtaa gtggagttct taaatcatga gaaattgacc taagcaagtt
    67561 tcctcttaac ctctctctct ctgcctcaag gtttgctttt ttatttttct caaataaatt
    67621 ttctctatca attgccaacg caacctgtgt tgaaactgct tctaatagta tcttttcatt
    67681 ttctgtaata atatctatct taaaacacgc aacacctata acaccaagaa catttgattc
    67741 ttgacctttt atagggaaat aatatgcact gctgttttta caatattttg tcccaattcc
    67801 aactgggtta cctgtcctaa aagcttcttt tatagcttcc ttttctaata ctgaatggaa
    67861 tatattctca cttccatcat tgttacaaat ataaatactt ggcttaccta gattattatt
    67921 ctcatctgca atagtcacta ttacagttct atttaatata tttactaaat tagacccaca
    67981 aaattcaata atttgatttt tattttttgc tttaagcaac gttttgttat tctcatagag
    68041 caatcttatc attttttctc tcttatgaga aagtctcact tctcttttta tcctagaagt
    68101 taaagtactt gttataattg ctgccattag cataatacaa aaggtcatag gataatcttt
    68161 tctataagct aaaaaagtat ataatggatg cgtaaagaaa aaattaaatg atagcactcc
    68221 tattactgaa gaaagtattc caaaaaaata tccataggtt atacttgtag caaatagtac
    68281 acctaatatg tatactagta taatattaga ttcattaaaa cctacctttt caaaaactac
    68341 tgatattcca gtagcaattg tcataaccaa tatagttttt atgaagtcct taataattat
    68401 ctttttactg agatagccat cttcatcata atcatactct tttaagtctt catgttgtag
    68461 tcgttccttt ttcatttact acaattcctc cttattgtta ctgtttaaat tggctctatt
    68521 ttatataata caattttatc ataataaatt caatcaatac atttaattaa tgtacttaaa
    68581 ttactaaaag gtgctgtctt aaaatgaaaa taaatctcta tttcaaaaca gcacctatat
    68641 atttaaatta ctaatttagt ttttacatac ttaaatcaat agttttattc aatttcataa
    68701 attataatac ttcaattata aaacatttct atccaacaag cataaatata aagtgggcac
    68761 atatacctgc acataaacca cctatagtat gcgaaagtat cgctttacca gccaatggtc
    68821 ttgcatctaa agcatccatc atacctatgt gtgtacttaa atatccactc caacacatac
    68881 ccattgctgt gaatactgct atatcattag gagttatagc gcctgatttt ataaactctg
    68941 gtactaaaga tattgctgct ccaacagctc ctaatgcagt tacagggaat gctattgcct
    69001 ctggtgatgt gaatccaaat aatggttcta ttatgaagct aatcttatca ccaatagcag
    69061 gtaataattt tataccttca tatgcagctc ctgtgtaaac agcttgacct gtagcagggt
    69121 ctgtagatgg tccaaaagtt aataacataa ctaatgtaca aactactaaa actcctggta
    69181 ttatagccat acccatttca acacctaact taccaccttc aagtatagca tccaatgttc
    69241 tttggaatac attaccatcc cttatttctc taaactcttc aagttttgca cctttatcat
    69301 ttatttgctt ttcagtatca tttttagggt cataattata atacttttta gtaaaagtca
    69361 acataattct aacactaata atactaccta ttattgcccc aacattacct attatagctg
    69421 gtagtacata ttctttacct tgagctatca taaaagttgt tacaataagt cccataccaa
    69481 aggctgttcc taaattacat aaagcaggta cttgatactt cttaaaatat tgtgtaaatg
    69541 ttttgtcttt agcaaatggt ataatagctg gattatcaga taaatatgtt gcaactactc
    69601 ctgctatact agcaccaggt aagccccata taggtctcat aagaccctta aatatcttat
    69661 taactaaaga tattactcca aactctgaaa gcaatgcact aagtgcacct gcaagaactg
    69721 ccatagccat tattaaaaat actgtgtcta ataaaagcgc atgtgctgta ctcattatta
    69781 ctttaaacat cattcctgca cccatgatgc ttcctacata tccaaatcct accgccaata
    69841 gtacaataaa tacgaaggtt tccatactta ctgccttgac tcttctctta tccgacagct
    69901 tttccatctt gaaatcttcc cccttttaat tttaaattaa ggttcctaac attggtacaa
    69961 attttttggt acaaactgag ttctaaaact tacactcgta atgcaagttg tcagtttttt
    70021 gaaataaaaa aaaattacgt ttatagaata agaatatcct ctttaatttg gatactctta
    70081 ttctatttta tgttattata ttatcatcct tttgtttaaa agtcaatttt ttctattttt
    70141 gttgtttaat tttatttata ctctttttat atttttcatt ttcttttatc tttgtaagta
    70201 ctccatataa gttgactttt ctaaatacag tagttataaa taccaatcct aatgcaattg
    70261 aacttgcaca cgataatgta ctcaccccat attctacagc ttttatataa ttattggtca
    70321 aaacagaata catagtatta tatattccat aaccaggtac taatattatt aaacagcaaa
    70381 cacttaatgt agttgctgga gtcatataaa ttcttgcaca taactcacaa tatatcgcaa
    70441 aacaaactgt agctataaaa aaacataagt tttctgttac tcccatattt aaaagaaatt
    70501 tatacgaaaa ccaagctata cttccaccta ttcctgctag tattaagttt tttcctttca
    70561 tattaaatag cactccaaag cttaaggcag taaaaaaagc tgctattact tctataaaca
    70621 aatgcactta tatcccccct aacttgaagt acaatccaag taccaagaac attcctactg
    70681 ctatagatac acctacaaaa aatacttctg ctaattttgc aagaccagaa atcaactgtc
    70741 catctaacaa atctcttaat gcattagtca aagcaagccc aggaactaag agcattattg
    70801 ctcctgctat taaatggtct aacgtcttta tgaatccgag ctttaaaatt aaaattgaac
    70861 aagaagctat aataactgct ccaattgcat ttacaaaaaa gtcattaagg gaagatttac
    70921 ttaaatacat aataacaaac ttagtcagaa tcccaacagt aaatgcagct ataaattctt
    70981 ccacatttcc tccaaataat atcgaaaaag ttgctgctgc aatacctgca aaaaatattg
    71041 ttattaaatc cgagtaatag ttatcttcac ttatttcatc taaaattttc tcacataatt
    71101 caatatctaa attatttata cttattgctc ttgataatga gttaacttca tgaaccaagt
    71161 ttaggtctac tcttcttgag cttattcttt taattgctga atgtaatttc ccatctacaa
    71221 atacagatgc cattacagct gttggagttg caaatacatc tacgtggtct attccaaaag
    71281 attgacatat tctagctatc gtttcttcaa ctctataggt ttctcctcca ctttgcaaca
    71341 tagctttacc tgcatttgag gaaaagttta aaattctatc tatatccaca aaacatatct
    71401 cccattcttt tatagtatta ttcatatttt aatacaagat attatttatt taaactattt
    71461 taaataattc atatccaata tttacatttt atgaaaaaac ttgtttttta tgcttgtcaa
    71521 aatcaaattt taagcttgtt tacaccataa aatggctgaa ttaatatttg acaaacactt
    71581 tcaaccattt atataacctt ctaaattgta taacttttta ctacaaataa aattatatct
    71641 cttttaattc tttctcttga gtatttagct tatagcaact tcatatttat atatgtacaa
    71701 tataaaacat tatttttatg atacaaaata taatttgtaa atgtatatat gtattaactg
    71761 tgtatatata aattattctt aaccttatca gttttttcat atatcttcat ctataccgac
    71821 catatattaa ttacattgac catatattaa taaatgatat tacatagact taagtatatt
    71881 aaaaaactta aatttagtta tttaacaaca taatactcta atttaatcta taaaaatcca
    71941 tacctaatat aatgcaactc ttattttaac accatctatt acacaaaaca acatattaaa
    72001 aagtaaaata caaaatatcc actataaacc ttattttaat accgaatata tattataata
    72061 tatttaatgt agcttaaata tagtttaaac ataaagaaag gaaacggtat atgctaagaa
    72121 aagataaagt tctattatct ctacataaat tatgtgaaaa tatgacttta aaagaccttc
    72181 aatcagataa aacaggattc actacaatag aaattgctaa ccattcatct cttgatagaa
    72241 gtaataccag taaagaatta aataacctat acaatgaaaa aaaagttctg aaaatatctg
    72301 gtaagccaat tttatttcta catgctagta ttatagaaaa tttaattggc cgtactttga
    72361 acagtgatga attttctata aataattgta atgagctact taaatcaagt aaaaatgact
    72421 cagaagatat tttttcaaca ttacttggac atgattctag tcttaaacta cctatagaaa
    72481 aagctaaatc tgctatactc tatcctccaa aaggtcttaa tacgttactc ataggaccta
    72541 ctggtgttgg aaaaagtaca tttgccgaaa caatgtacaa atatgctgta gaaagtaaaa
    72601 tcttttcttc tgaaagcaaa ttcgtagtat ttaactgtgc tgaatatgca gaaaatgcta
    72661 acttattatt aagtaattta tttggttatg tcaaaggttc ttttactggt gctaataagg
    72721 ataaattcgg catagttgca caggctgatg gaggcatatt attcttagat gaaatacaca
    72781 gattaccacc agaaggtcaa gagatgcttt ttcttttgat ggacaaaggc atatacagac
    72841 gattaggaga atctgacatt gtgcataaag ctaatgtctt tattgtgtgt gccacaacag
    72901 aagatataca ttcttctctg cttggaactt ttttaagaag aatccctgtt acaatcaacc
    72961 tacctgcttt acaggatagg tctttaaaag aaagattatc tcttattaaa tacttcttta
    73021 ttatagaatc aaaatacacc aatgcaacta taaaagttca caaagatgta attaaatcat
    73081 tactccttta tgattgtttt ggtaatatag gtcagcttaa atctgacata caacttatga
    73141 gtgcaagagc cttcttaaat tacaaaactg gacttaagga taatatagaa atagatttat
    73201 cccttgttcc tgattacatc tatattggtc ttttaaaatt gaatgaaaat cgtagtaatc
    73261 taaataaatt atcagaactt gattatatgc ttttttatga atttaatggt aataacttag
    73321 aagttgaata tacaaaagaa ctatctaaat acaatataga tacatcaaac aatggattta
    73381 aatactctac agagattcct tatctggaaa atgatataag gagtatgttt ataaattaca
    73441 tggaaaagta ctctacaaaa ttactttcga tatcttcagt aactcattct gattatacaa
    73501 gtaatgagat ttttaaagta atcaatccaa agatttacta tgctgttgaa cattctttaa
    73561 aagaggctga aaaaatatcg agaaacaaat acacaaaaca aacttatatt gctctttcaa
    73621 tgcatataag tgcacttata gaaaatataa atcaagacac atataatcct aagtacaatt
    73681 tatataatga taaaattttc ttagataaag atttaaatat agctaaaata gttttaagta
    73741 taattgaaaa tgacttacaa acatctttac ctaaagatga agtaaaattt atatccatgt
    73801 ttctaaatag catgtatctt gaaccaaaca atcaatctga aagtatagga gtcataatac
    73861 ttgctcatgg aaattcaaca gcaagcagta tgtgtgatgt agcaaattca ttgcttggta
    73921 caaaccattg taatgcaatt gacatgcctc ttgatgtaaa agtaagtacc atacttgata
    73981 aaactataga gttggtaaaa gaatgtgacg aaggtaaggg tgtacttctt ttaacagata
    74041 tgggctctct tatagctttt ggagaactta tttcagaaaa aacaaaaatc aaaactcgct
    74101 caattgaaat ggtatctact ccaatagttt tagaagcagt aagaaaaagt acactaccag
    74161 aaatgacttt ggatgaatta gctaattcct taagctcttt aaatccatat attggacgag
    74221 cagcaatcga ttcaacagaa ataaactatc ctgaaaaatc aagagagtat gtaataataa
    74281 ctacttgtat aacaggatat ggttcagcta tgaaagtagc agactttata aattcttcac
    74341 ttaaaaatat tagtaaatac aatattaatt tagttcctca taatatagaa agttttaaaa
    74401 tgtataagaa taaatttagt aatactaaaa ttctggcagt tgttggaagt gtagatttta
    74461 atgtagccaa cacccctttt atacacatag aggaactact aaatggatat ggcctagata
    74521 tgctaaacaa aataatagaa ggtacattca atgccaatag tattacaaat ataaaagaaa
    74581 caaatctatc atacaacagt ttaataataa actttatgac ccaaaactta gagcttttgg
    74641 actctgttaa attattcaac tatgtcaatt cttccttatt tacaataata gaattaatag
    74701 atgaaaactt aaagagcaaa tttaagctag gatatatact acattgctct tttatgatag
    74761 aaagatgttt taaaaataca acttttccat ataataacat tactgaatta attagtaaaa
    74821 atcatgaact atatagttta atacgtgaag cctttaagcc aacagaagct tattttaata
    74881 ttttgatacc agacgaagaa attgcttata taatagacat gattaatgag tatctaaata
    74941 aataaaattt atagcactta ttttgtgcat attaattaat atatgaaatg gaggacgaga
    75001 ttttgaaagg aaaaataaaa cagttaacaa tacttgcatt aatttttata tttataactc
    75061 ctgtatttgc atttgcagat actccaccag taccaaattc ttcaagagca gcactactta
    75121 tagaccagga aactaaaaga atattatttg aaaaaaacat tgatgaaaaa atgccattag
    75181 cgagtttaag taaaatgatg acctttttat tggcaataga agcagttgat aaaaatcaag
    75241 ttaaagagac tgatatggtg aaaatagaca agtctacagc ttcagtagga ggttctactt
    75301 gtaaacttaa agatggtgat gaaatatctc taggagagct tatgcagggt ctaatgctag
    75361 tatctggaaa tgatgctgct atagccatag ctaaacatat tggtaaaact gaaaagaatt
    75421 ttgtaaatat gatgaacaaa aaagcagaag aaataggaat gattgatacg tattatttta
    75481 atccaaatgg gcttcctata tatactgacc cagaacataa agaacctcct attgaaaata
    75541 tgtcaactgc tcacgatata gttacacttg gaaagtatat gtacgaccat tatgaaaatc
    75601 aagttacaag aataaccact atgcaagtat ataatgatac taaaaaggat tttacacatt
    75661 ataacacaaa tcctctgctt gtttcagtac ctggtgttga tggaataaag acaggatata
    75721 cagataatgc tggatattgc cttgcatttt ctatgatggt acctaaagat gctaaaaacg
    75781 aaagaaatca tagattgata ggtgttgtac ttggtgatgg taacaagaaa aatagaattt
    75841 cttcttccgc tactttattg aaatatggta aagataattt ccattctaaa aagatagcac
    75901 ataagggtga tattatagag actccttgtg ttgatggtat tgatgatttt aagataacag
    75961 ttaaggttga taaagatttg tatggtgttg tttcagataa cgaaaatatc aatcctaagg
    76021 tagtgtttaa aaatatgaat tatcctatcc ataagggaga tattgttggt gttgcaaagt
    76081 attataatga ctctggtaaa tttgttggaa gtgttgatgt aaaaagtgaa agtaatatag
    76141 gatgtattcc tttgaaggat aagattaaga ttaaggttgc taagataaat aaaaaacttg
    76201 agattaaaaa ttctgtttgt tttaaagcat agtaaagcat attattaaaa gggtatgtta
    76261 aatttataaa gactccactt tgtggagtct ttttgttttt tacaaaaaat taaaatagtg
    76321 gcaacaattg aggatgtgag ggagagttgc cactattttg tatagggtat tatattatgt
    76381 ttttaagcta gttttactat cttctatgtt tatataataa ctcttgtgtg tgaatttaat
    76441 tgataattaa tatgaaaatt atgtgaagtt ttgatttatt tgtaatatat tctactacta
    76501 ttataatttt tctactggat atactaatca tttgtttaac attatctgag attgatttgc
    76561 aaacttttat ataactaaat tagttatata acttatagat tccattaaat accctaataa
    76621 tcatatatgt acatacaaag tcatgcttat gcaaaatctg cagtttcaac taacttatta
    76681 tataaaccat ctttatgtag aagttcagca tcatttccag attctattac tctaccttta
    76741 tctaatacat atatattatc agcactctta attgttgaaa gtctatgagc tattataata
    76801 actatcttat cttgtatgta ctctttcaag ttgttcaata tctctttttg tgaaatatta
    76861 tctagagctg aagttgcttc gtccagaata agtacctttt tattacttac taaagctcta
    76921 gctattgata tacgttgttt ttgacctcct gatattttga cacctttttc tcctactact
    76981 tcgtccaatc ccttatctag attttttata aaatcatcta aacaagtaac ttctaatatc
    77041 tcatctaatt ctttatcact tattttatta tttaatacta tattattttt tattgtgtca
    77101 tcaaatacaa attgatcttg agttactata tcaatattat ttcttagtga ttttaaatta
    77161 atatccttta tattaacacc atctattaat atatcacctt catttatatc ccaaagtcgg
    77221 tatattagat ttgttattgt tgatttgcca catccacttc caccaacaat tcctgttact
    77281 tcccctttct caaattgtaa atttatatca tttaaaacaa ggtttaaatc ctcaccatat
    77341 ctaaaagata catttttaaa atctattttc ttaatttcac tgttatctat tctatatcca
    77401 ttattatcaa ctttaatatc tatcttttca tctattactg aaaatactct attgatagac
    77461 acttttgatt gtgctattct agtattcgcc cttactatag ctatacatgg tccaattaac
    77521 attcctgtat attgttgaaa tgctattaac tcccccatcg tcatcttatt atttattatt
    77581 ttatatcccc caattccata tattaatatt gttatcaatc ctgttagtat ttgagatatt
    77641 gctatattac tactcataat gatgttcaaa ttcaaatctt tttttatcaa atctctctct
    77701 ctttaaatac ttattaaaga aattcttttt tgaatttaat ataacaatat ccataatatt
    77761 agatacgtac tcttgaatca aatttgataa actgcctgaa tcacttctta tttcagaaat
    77821 tttcttagat ataagttttg tcattttatt ttgagaaaat gttaatatca actgtaaaaa
    77881 taaaactact aaaaataaat caaactgcaa attaattaag aaaattaacg atattatagc
    77941 tgtaaacaca tttacaataa tatcaaatat aatctctgct ccaaagtttt ctatcataaa
    78001 tatatcactt tccataatgg ataaaatatt acctgttttc ttacttgaat aatatctacc
    78061 agaaagtttt gatatatggt taagcaactt tacttttaga tttatagtaa ctttattttt
    78121 tattgaagta tataaatatg ataatataac atcaaatgta gctgataata tccttataaa
    78181 aatatatatc aatattaaat ttataagcag aaaaatattt ttat
//
LOCUS       DAEMGG010000003        74840 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEMGG010000003 DAEMGG010000000
VERSION     DAEMGG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 74840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.003"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            101..1234
                     /locus_tag="KPK24_000161"
     CDS             101..1234
                     /locus_tag="KPK24_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG2297882.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGIEYYDSLLND"
     gene            2150..3172
                     /locus_tag="KPK24_000162"
     CDS             2150..3172
                     /locus_tag="KPK24_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2297883.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            3469..4389
                     /gene="pyrB"
                     /locus_tag="KPK24_000163"
     CDS             3469..4389
                     /gene="pyrB"
                     /locus_tag="KPK24_000163"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG2297884.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            4428..5120
                     /locus_tag="KPK24_000164"
     CDS             4428..5120
                     /locus_tag="KPK24_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG2297885.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            5113..6015
                     /locus_tag="KPK24_000165"
     CDS             5113..6015
                     /locus_tag="KPK24_000165"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG2297886.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            6100..6684
                     /locus_tag="KPK24_000166"
     CDS             6100..6684
                     /locus_tag="KPK24_000166"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG2297887.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            6899..7825
                     /locus_tag="KPK24_000167"
     CDS             6899..7825
                     /locus_tag="KPK24_000167"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBG2297888.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(7877..8767)
                     /locus_tag="KPK24_000168"
     CDS             complement(7877..8767)
                     /locus_tag="KPK24_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2297889.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            8955..9986
                     /locus_tag="KPK24_000169"
     CDS             8955..9986
                     /locus_tag="KPK24_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG2297890.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            10280..11155
                     /locus_tag="KPK24_000170"
     CDS             10280..11155
                     /locus_tag="KPK24_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HBG2297891.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            11173..12663
                     /gene="cls"
                     /locus_tag="KPK24_000171"
     CDS             11173..12663
                     /gene="cls"
                     /locus_tag="KPK24_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG2297892.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            12856..13140
                     /locus_tag="KPK24_000172"
     CDS             12856..13140
                     /locus_tag="KPK24_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG2297893.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            13173..14801
                     /gene="groL"
                     /locus_tag="KPK24_000173"
     CDS             13173..14801
                     /gene="groL"
                     /locus_tag="KPK24_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG2297894.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDVGMPGMGGGMPGMM"
     gene            15063..15803
                     /gene="larB"
                     /locus_tag="KPK24_000174"
     CDS             15063..15803
                     /gene="larB"
                     /locus_tag="KPK24_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG2297895.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            15887..16621
                     /gene="larC"
                     /locus_tag="KPK24_000175"
     CDS             15887..16621
                     /gene="larC"
                     /locus_tag="KPK24_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG2297896.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            16651..17070
                     /locus_tag="KPK24_000176"
     CDS             16651..17070
                     /locus_tag="KPK24_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG2297897.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      17130..17231
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            17452..18987
                     /gene="guaA"
                     /locus_tag="KPK24_000177"
     CDS             17452..18987
                     /gene="guaA"
                     /locus_tag="KPK24_000177"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG2297898.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            19526..20956
                     /locus_tag="KPK24_000178"
     CDS             19526..20956
                     /locus_tag="KPK24_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2297899.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(21104..21454)
                     /locus_tag="KPK24_000179"
                     /pseudo
     CDS             complement(21104..21454)
                     /locus_tag="KPK24_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            21449..21970
                     /locus_tag="KPK24_000180"
     CDS             21449..21970
                     /locus_tag="KPK24_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2297900.1"
                     /translation="MFSIGGGYAIILLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(22099..22893)
                     /locus_tag="KPK24_000181"
     CDS             complement(22099..22893)
                     /locus_tag="KPK24_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2297901.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAKDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(23117..26545)
                     /gene="uvrA"
                     /locus_tag="KPK24_000182"
     CDS             complement(23117..26545)
                     /gene="uvrA"
                     /locus_tag="KPK24_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2297902.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHQDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLKGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            26864..29098
                     /locus_tag="KPK24_000183"
     CDS             26864..29098
                     /locus_tag="KPK24_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2297903.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            29354..31384
                     /locus_tag="KPK24_000184"
     CDS             29354..31384
                     /locus_tag="KPK24_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2297904.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKDWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            31381..31833
                     /locus_tag="KPK24_000185"
     CDS             31381..31833
                     /locus_tag="KPK24_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2297905.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            31835..32890
                     /locus_tag="KPK24_000186"
     CDS             31835..32890
                     /locus_tag="KPK24_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG2297906.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            32957..33265
                     /locus_tag="KPK24_000187"
     CDS             32957..33265
                     /locus_tag="KPK24_000187"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG2297907.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            33369..34634
                     /locus_tag="KPK24_000188"
     CDS             33369..34634
                     /locus_tag="KPK24_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG2297908.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            34722..35438
                     /locus_tag="KPK24_000189"
     CDS             34722..35438
                     /locus_tag="KPK24_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2297909.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(35633..35926)
                     /locus_tag="KPK24_000190"
     CDS             complement(35633..35926)
                     /locus_tag="KPK24_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG2297910.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(35932..36147)
                     /locus_tag="KPK24_000191"
     CDS             complement(35932..36147)
                     /locus_tag="KPK24_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297911.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAMQQFIVENEILISEEV"
     gene            36596..36994
                     /gene="tnpA"
                     /locus_tag="KPK24_000192"
     CDS             36596..36994
                     /gene="tnpA"
                     /locus_tag="KPK24_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG2297912.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            36991..38154
                     /locus_tag="KPK24_000193"
     CDS             36991..38154
                     /locus_tag="KPK24_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2297913.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(38538..39050)
                     /locus_tag="KPK24_000194"
     CDS             complement(38538..39050)
                     /locus_tag="KPK24_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2297914.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            39500..39976
                     /gene="purE"
                     /locus_tag="KPK24_000195"
     CDS             39500..39976
                     /gene="purE"
                     /locus_tag="KPK24_000195"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG2297915.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            39982..40680
                     /locus_tag="KPK24_000196"
     CDS             39982..40680
                     /locus_tag="KPK24_000196"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG2297916.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            40692..42059
                     /gene="purF"
                     /locus_tag="KPK24_000197"
     CDS             40692..42059
                     /gene="purF"
                     /locus_tag="KPK24_000197"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG2297917.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            42053..43129
                     /locus_tag="KPK24_000198"
     CDS             42053..43129
                     /locus_tag="KPK24_000198"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG2297918.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            43123..43716
                     /locus_tag="KPK24_000199"
     CDS             43123..43716
                     /locus_tag="KPK24_000199"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG2297919.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            43709..45241
                     /gene="purH"
                     /locus_tag="KPK24_000200"
     CDS             43709..45241
                     /gene="purH"
                     /locus_tag="KPK24_000200"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG2297920.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAVVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            45252..46502
                     /gene="purD"
                     /locus_tag="KPK24_000201"
     CDS             45252..46502
                     /gene="purD"
                     /locus_tag="KPK24_000201"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBG2297921.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTRVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            46527..50333
                     /locus_tag="KPK24_000202"
     CDS             46527..50333
                     /locus_tag="KPK24_000202"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBG2297922.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDNKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVIGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            50539..51411
                     /gene="rfbD"
                     /locus_tag="KPK24_000203"
     CDS             50539..51411
                     /gene="rfbD"
                     /locus_tag="KPK24_000203"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBG2297923.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            51520..52389
                     /gene="rfbA"
                     /locus_tag="KPK24_000204"
     CDS             51520..52389
                     /gene="rfbA"
                     /locus_tag="KPK24_000204"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBG2297924.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            52438..52995
                     /gene="rfbC"
                     /locus_tag="KPK24_000205"
     CDS             52438..52995
                     /gene="rfbC"
                     /locus_tag="KPK24_000205"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBG2297925.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            53033..54016
                     /gene="rfbB"
                     /locus_tag="KPK24_000206"
     CDS             53033..54016
                     /gene="rfbB"
                     /locus_tag="KPK24_000206"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBG2297926.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            54046..54714
                     /locus_tag="KPK24_000207"
     CDS             54046..54714
                     /locus_tag="KPK24_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG2297927.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            54723..55151
                     /locus_tag="KPK24_000208"
     CDS             54723..55151
                     /locus_tag="KPK24_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG2297928.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            55175..55489
                     /locus_tag="KPK24_000209"
     CDS             55175..55489
                     /locus_tag="KPK24_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG2297929.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            55641..55916
                     /gene="flgM"
                     /locus_tag="KPK24_000210"
     CDS             55641..55916
                     /gene="flgM"
                     /locus_tag="KPK24_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG2297930.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            55921..56337
                     /locus_tag="KPK24_000211"
     CDS             55921..56337
                     /locus_tag="KPK24_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG2297931.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            56355..57665
                     /gene="flgK"
                     /locus_tag="KPK24_000212"
     CDS             56355..57665
                     /gene="flgK"
                     /locus_tag="KPK24_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG2297932.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            57684..58616
                     /gene="flgL"
                     /locus_tag="KPK24_000213"
     CDS             57684..58616
                     /gene="flgL"
                     /locus_tag="KPK24_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG2297933.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            58632..59024
                     /locus_tag="KPK24_000214"
     CDS             58632..59024
                     /locus_tag="KPK24_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG2297934.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDSGC"
     gene            59018..59230
                     /gene="csrA"
                     /locus_tag="KPK24_000215"
     CDS             59018..59230
                     /gene="csrA"
                     /locus_tag="KPK24_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG2297935.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            59252..59647
                     /gene="fliS"
                     /locus_tag="KPK24_000216"
     CDS             59252..59647
                     /gene="fliS"
                     /locus_tag="KPK24_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG2297936.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            59647..60003
                     /gene="fliS"
                     /locus_tag="KPK24_000217"
     CDS             59647..60003
                     /gene="fliS"
                     /locus_tag="KPK24_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG2297937.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            60024..61547
                     /gene="fliD"
                     /locus_tag="KPK24_000218"
     CDS             60024..61547
                     /gene="fliD"
                     /locus_tag="KPK24_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG2297938.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            61550..61885
                     /gene="fliT"
                     /locus_tag="KPK24_000219"
     CDS             61550..61885
                     /gene="fliT"
                     /locus_tag="KPK24_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG2297939.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            61987..62859
                     /locus_tag="KPK24_000220"
     CDS             61987..62859
                     /locus_tag="KPK24_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBG2297940.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            62952..65093
                     /locus_tag="KPK24_000221"
     CDS             62952..65093
                     /locus_tag="KPK24_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2297941.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            65117..66994
                     /locus_tag="KPK24_000222"
     CDS             65117..66994
                     /locus_tag="KPK24_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2297942.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            66987..69101
                     /locus_tag="KPK24_000223"
     CDS             66987..69101
                     /locus_tag="KPK24_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG2297943.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            69110..70105
                     /locus_tag="KPK24_000224"
     CDS             69110..70105
                     /locus_tag="KPK24_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBG2297944.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            70197..71429
                     /locus_tag="KPK24_000225"
     CDS             70197..71429
                     /locus_tag="KPK24_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBG2297945.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            71449..72393
                     /locus_tag="KPK24_000226"
     CDS             71449..72393
                     /locus_tag="KPK24_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBG2297946.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            72487..72894
                     /locus_tag="KPK24_000227"
     CDS             72487..72894
                     /locus_tag="KPK24_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HBG2297947.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            72981..74072
                     /locus_tag="KPK24_000228"
     CDS             72981..74072
                     /locus_tag="KPK24_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBG2297948.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
     regulatory      74381..74471
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT        29716 a         7832 c        13264 g        24028 t
ORIGIN      
        1 caaaaaatgt atctttttaa ttaataaaga taatatgaaa acttaaaagg caaataaact
       61 taaatataaa atactcttaa ataagatagg agaaaaatgt atgtctaact ttattctaaa
      121 tgggattttt agaacagtta tagtaagtag tctaagcatt cttctgatat tgattttcaa
      181 gaaaaatgta tttaaaagat ttagcaaaaa atttaattat tatatatgga tgatagttgt
      241 tattaaatta tttttaccat ttacatatta tacttttaca ataaatatct tgagaagtaa
      301 aaaacacatt aatattaata acataaattt agagggattt aataatgttt caactataag
      361 taataatatt atattgtaca cttggattat aactgtagta gtgtatttaa tttacactat
      421 atttaaatat ataaaactta aaaatttaat aaatgactta tcttatgatg ttgatgatag
      481 agagattgta aatctttata aaaatatatt agaagaattt aatataacaa aagatataaa
      541 actcaaatac tcttatgaag ttgaaacacc agcatttttt aattcatgtg tacttttgcc
      601 accacatgag tataaattga aagagttaga ctggattttt agacatgagc ttatgcattt
      661 taagagtagg gacctttatt taaaatatgt aatcctattt ttaaaaacag tatattggtt
      721 taatccattt atatatataa tggataaaca tatagattta gattgtgaac tttattgtga
      781 tgaaagagtt ttaaaaaatc gaaattcaga ggaaaaaaag gattatgctt taacaattct
      841 aaatgcaatg agaaaaggtt caaatacttc aaataaattt atagctggac ttcataagca
      901 atctgatatt aaaaagagag ttatgaatat gtttaatgaa aagtacaaga atggtatatt
      961 aatggcatta actttatgtt tattatcttc tattactttt ttgaaggtta attcaactag
     1021 tattgttaat cttaatgtat tacctaaagg atttgaagac ttacgaactg ttagcagtat
     1081 acgtttttca gttataggat ttacatatgc agatgctcct aaaaatatga gggaagaata
     1141 taaaataaat tgtgaatcat taggtttgat acctaaagac tctgataaaa tagaagttgg
     1201 tatagaatat tatgacagtc tattgaatga ttaaataaat attattttaa cagatattaa
     1261 ttataaagtt ttgaatagta atttgttgag aaataaatat atccttataa aaatagatac
     1321 ttatatacca agaataaaaa atagtcaaga tagtacacaa ggatatactt ataaagaact
     1381 taaataagtg aaataaatta ttaattataa ggtttataat tttaaaaaat tagataaatt
     1441 caggtgtaaa atactataaa acacaattcg gaaaagtgta tgttgaactt catttaaaat
     1501 ggaatttcaa agttgctata gtaagtagtt taagcattct tcttatatta atctttgaga
     1561 ataatgtatt taaaaaagtt agtaaaaaat ttaactattg tatattaatg atagttgcta
     1621 ttgatatatt atctaaaaaa attaagtatg aaaagattac tgctaggaca atcttttcat
     1681 acttaatttt taatatttat attatccaaa attttcatct ttttaactta gaatttgaga
     1741 atcactatta ggaaataggc tttgatttat gtagggttaa aaatttcaca gaatatctaa
     1801 ctttatacta aatccatcaa aaaatatacg ccatacaaaa gcgcataaaa aaacacataa
     1861 aacaatattt atgcgcactt taacctcgaa tggtacaaaa ttctttactt ttacagaccc
     1921 atattatata attaagtaaa taatgtatat ttaggtgaca tattggataa gctaatttat
     1981 ttattttatt aatataataa tactataaaa gttatataaa gtggtttaca aaaaagttac
     2041 agatagagct tttattaatg tattgtgtat tagtatttta tccttaggta cacttcacct
     2101 acaaaataga atcataagat aattatggga aaattttagg aggtttatcg tgaagaaaaa
     2161 gatattaata ccagtatttg catcagttat ggcattatca gtaagttcaa ttgtaaatgc
     2221 agatgaagta aatgattcat ctcaaaataa ggatgataaa acaaatactg aattaaattt
     2281 aggtgaatat aaagaagtaa aatataaagt tgcaaaaata aaagatggag tagctataaa
     2341 gataagagaa gaaggtcaag ttcaaaacat agcttattct ggagatgaat ttacggtttt
     2401 aggaactcaa ggagaatggg ttaaggttaa agttgaagat ggcgaaggtt ggttagctac
     2461 tagatatgta gatatatctg aaggtgtagg atatactaac gctgataaag taaacttaag
     2521 aaaagataag agtgaaagtt cagaagtaat tgaagagctg gaaaaaggaa gttctttatt
     2581 ggttttggaa gataatggag attggttaaa agttaaagat ggggaaacag aaggatatgt
     2641 aaaatcatct tatatatctg ataaagctcc tgtaatagaa gagccacaag taaacccaag
     2701 tattgaccaa aatgtagatt ctacaaataa taatcaacaa aacaatgcaa atactaatca
     2761 acaaaataat aattcaaatg tgccaacagc taactctaat gcagttcaag ctgtacttaa
     2821 cttggcatat tctaagcaag gttgtccata tgtttgggga gcagaaggac caaacacttt
     2881 tgactgttct ggatttacac aatatgtgta tagaaatgca gtaggaaaga atataccaag
     2941 aacatctaaa gcacaatcta aatatggaca aactgtaagt aaagcaaatt tacaaccagg
     3001 agatttagta ttcttcacaa ctaatggttc tggttctgtt agtcatgttg gaatatatgt
     3061 aggtgggggt aatatgatac actcaccatc aacaggaaaa acagttagtg taactagtat
     3121 aaattcatca tactatacag caagatttgt tacagcaaaa agaatattat aatcggtttg
     3181 taatatgaca aattgataaa tacataaata tatataaacg ctctatatta tatttaattt
     3241 aatcatggaa acaaaataat gattatattt taaatgtact atagagcgtt tttgtatcac
     3301 tttaaatcta atcaagattc aagttaatat gactgttaaa taggagcctt ttcatatgat
     3361 gattatttgt gcaatccctt gacaggatag ggtaaaggtg gtaagctact aacagtacaa
     3421 aaatactttt aattttagtt cagagaggct aaaaaaggag gacgaaagat gttaaaatca
     3481 agaaatttaa tccaaccaga agacttttca atagaagaaa tagatgaaat attagaatta
     3541 gctcaaaaga taattgacaa tccgtcaaaa tattctcgta tatgtgaagg aaaactatta
     3601 gctacattat tttatgaacc atcaacaaga acaagattaa gctttgaatc tgctatgaac
     3661 agattaggag gacgtgtagt aggcttttca gagccaaatt catcctctgc atcaaaaggt
     3721 gaaactctag gagatacaat gaggatagta tctggttatg ttgatataat agctatgaga
     3781 catcctcaat caggtgcagc ttcagaagca gctagatata cggaagtgcc atttataaat
     3841 gcaggagatg gcaaaaacca gcatccaact caaacactta cagacttact tactataaaa
     3901 tcattaaaag gaactttaga aagccataca ataggattat gtggtgattt aaaatatgga
     3961 agaactgtgc attctttggt taaggctatg gcaagatata aaaatacaaa gtttgtattt
     4021 atagcacctg aagagttaaa aatgccagat tatataaaag aagctataaa gggacatgca
     4081 tattatgaaa ctaataattt ggatgatgtt ataggaagct tggatgtttt atatatgact
     4141 agagttcaac aagaaagatt tgaagataaa tcagagtatg aaagacttaa aaactattat
     4201 attttaaata aagctaaatt agaaaaggcg tctaaagata tgttagtaat gcatccatta
     4261 ccaagagtaa atgagataga catagatgta gatagtgatg atagagctgt atattttaaa
     4321 caagctaaat atggaatgta tgttagaatg gctttaataa taaaactttt aggtataaat
     4381 gaagactaat agttatcata taatagtaaa acaaaggaga ttttgggatg tataaaattc
     4441 tggaaaatat atatataggc gaagatatgt atagaatgaa agtaaaaggt aattttgaag
     4501 gtaaaatggg acaattctat atgttaagag catgggatac ttatccagta ctttcaagac
     4561 ctataagtat acatgatata gatgaagaag ggataacttt tctttataaa gtagtaggag
     4621 aaggaactca aattttgagt aacctaaagg taaatgatac tataaaattg gaaggccctt
     4681 atggtaatgg atatgctaag gttgatggaa aggtagctct agtaggagga ggaatcggtg
     4741 tagctccact atatttggtt gctaaaaata taaaaaattg tgatgcatat cttggcttta
     4801 gagaagatgt tatattagaa gatgaatata aacaagtatg taataaagta tatacaacag
     4861 taggaaatac ttttgtaaca gatataattg atgttgagaa atatgattat atattaacat
     4921 gtggtccaac tccaatgatg gaaaaattag ttaagatggt agaaggtact aaaacgagaa
     4981 ttatggtatc tcttgaaaac catatggctt gtggagtagg tgcctgttta gtatgtacgt
     5041 gtaaaactaa tggaggaaat aaaaagactt gtaaagatgg tccagtgttc tggggagagg
     5101 acgtgatttt taatgggtaa tttaagtgtg aaatttggaa gtgtagaatt taaaaatcca
     5161 gtaataatgg cgtctggaac ttttggattt ggaaaagaat ataatgagat ttatgatata
     5221 caaaaacttg gtggtataag tagcaagggt cttactttaa ataaaaaacc tggtaacaat
     5281 ggtatgagag ttcatgagac accatccggt atgatgaata gtgttgggct tgaaaaccct
     5341 ggagttcaag gatttataga ctatgaatta ccttttttta gtaaactaga cttagtcaga
     5401 atagctaatg ttggaggtgg gacattagaa gactacttat taggggttca aatgttaaat
     5461 gacaagccta tagacattat tgaattaaat atatcttgcc caaatgtcaa agctggtgga
     5521 atggcttttg gtataaaaaa tgaagtggca agagaagttg ttcgtgaggt aagaaacata
     5581 acgaaactgc ctttagtaat aaaattatca cccaatgcag aagatattgt aggaatggca
     5641 aaagtttgtg aagaagaagg tgctgatgga gtttcattag ttaatacttt taaagcaatg
     5701 gcaattgata taaaaaatag aagaccagta tttgaaaatg tatatgctgg gttatctgga
     5761 ccagctataa aacctatagc tttgagaatg gtacatgaag tgtgtaaaaa tgtaaatata
     5821 cctgtaatgg gaatgggcgg aataactaaa gccacagatg ctatagagtt tataatggca
     5881 ggagctactt gtatccaagt tggaactgca aattttatga atcctagaat aggtattgaa
     5941 ataatagatg gaattaatga atttatggat agagaaggaa taaaaagttt agacgaaata
     6001 agaggaatta tataaggtag aataaatagt attttaaaga taaaaaatat agattaaata
     6061 aacaataatt ttataacatt aacatggagg aaaaataata tgagtaaagt agatgtagtg
     6121 gatatattaa agaaaagtga tgcattgttg gagggacatt ttttattatc ttcaggaaaa
     6181 cacagtaata gatacgtaca atgtgcaaaa gtattaagat ttccagaata tgctgctgag
     6241 gtattaagta cagttgttga acaaataaaa gacttagata tagacttagt agtaggacca
     6301 gctatgggtg gagtaatagt ttcttatgag ttaggaagac aattaggaaa agaagctgta
     6361 tttactgaga gaaaagacaa tacaatggag ttaagaagag gatttgaagt taaaaaagga
     6421 gcaaagataa taattgctga agacgttgta actactggta aatcaactat ggagacaaaa
     6481 agagtattag aagccttagg tggagaagtt gtaggtgttg catgtatagc agatagaact
     6541 aatcatgata taggtatgcc tatatatagt gctataaaac ttgatattca agtttatgaa
     6601 tctgatgagt gtcctttatg taaggaagga aaattaccag ttgttaagcc tggaagtagg
     6661 gagttcaagg aattagggat gtaataaaaa cttaattatt tatagtgtta cttaaaaaat
     6721 gtaaattttt tagagtaaaa atttgtaata taacatataa cttaaaaata aaaagcagtt
     6781 agccttataa atgaaggtta gctgtttttt ttgttaaaaa tatatttaca aaataagaga
     6841 tagtagcagt acaatataag tataaggtaa aagtattata tattagggag gggtaagtat
     6901 gaaaaatcaa ttaaatgaaa atcataaaaa ggttgtattt tttggagtag gtgctgtagg
     6961 agctactttt gcagaacaat tttttaactc taaatatgat tttaaaattc tttgtgataa
     7021 tgaaagaaag aaaagatatt tagaagaagg atttataata aatggaaaaa ggtatgattt
     7081 tgattatgta actaaagatg agtataaaca agaggctgat tttataatta taggtctaaa
     7141 atataataat ttaaaagaaa atataaaaga attagatgga ttagttggaa aaaatacagt
     7201 tataatgtct ctgctaaatg gagttgatag cgaagagata ataggagaaa gatttggaat
     7261 tgaaaaaatg gtatattcat atgttaccaa tatagatgca aagaaaatca ataataatat
     7321 tatacacact actaatggga taattgtatt tggtaacaaa gataatagtg aagatagaaa
     7381 aactaatata ataaccgaag tctttgatga tgtaaatata gaatatactt tatcaaaaga
     7441 tattcaacga gatatgtggt ggaagtacat ggttaatatt ggtgtaaatc aaacttcagc
     7501 tatacttggt gcaccttatg gagtttttca gagttctgag catttaagag aattagcaaa
     7561 atctgcaatg agggaagttg ttgctatagc acaagcaaaa gacatatctc ttacagaaga
     7621 tgatgtggaa cattcattac atagaatact agaacattca aaagaaggaa gaacatcaat
     7681 gcttcaagat gtggaagctc atagacttac agaagtagat atgttttcta agaatatctg
     7741 caaacttgga aaaaaatata acatacctac tcctataaat cagactttct tttatatgat
     7801 aaaagtaatt gaaagtagat tttaaagggt ctttgtaaga aatattgata attattgatt
     7861 ttaaatttta ttaatgttat atactattta agttactaaa tatttttaac tcttcaatga
     7921 aaatagttgc tgcttttgat aaagttatat cttttggaaa tatatatgag aaatgtctgt
     7981 tgtagctgct tccaagctct atgacagaca gctcattgtt tagaacaggt aagcttgtca
     8041 ctagattaga gactattgta attcctaaat tatttctaac agattctttg acagcataat
     8101 tacttccaaa aaccatcata ctttttggaa taatttcttt aacacttaaa aacatatcta
     8161 aataatctct ggttccagaa ccatcctccc tgactatcca tttttggttt tgaagtttat
     8221 caaaactaaa atcttttaat aaatgactct tgtatggaag tgctaaaacc atcttatctt
     8281 caaaaaaata ttcttgaata aatgaagggg aagagcaagt gccttcaata agaccaatgt
     8341 ctaaaatata atccttaaca tgagaagata cgattgatgt attttttata aaaacttcta
     8401 catctatgtc aggatacttt ttagaaaata atgctagaaa atttggtaaa atatactctc
     8461 caatagttaa acttgcacca atctttaagt gaccagttat agcatctgaa acttggctta
     8521 catcatgata agtaatattt aaaagattta atatttcttt agctcttttg tacaagattt
     8581 gtccactttc tgttataaaa atggtttttt gtttaataga cctgtttata agcacaactt
     8641 taaaataatt ttctagattt tttatgtgtt tacttacact tggttgtgat agatttaaat
     8701 attccccagc tttagtaaag tttttgtatt caacaacagc tacaaatgtt tttaattctt
     8761 caaacaattt tttgtctcct ttacctaatg aaattttaca ttttttatat actttctaca
     8821 gataattata ctatatataa taaccattaa aaacattaat cattacgata ttatatattt
     8881 attttactta tggttattac aattatatac tattgctatg aagaaaacta tattaattat
     8941 aaggtaggta tattatgtta aagaaaaaaa gcagtttgac attaaaaact ataagggaaa
     9001 tattaccagg actgtttgta tcagtattag taggttatat tagtatattt atttctattt
     9061 taattcctaa ggtaggggca gcatcaattt ctatattttt aggtatgttt gtaggaaacc
     9121 tatttttaaa tcaaaaggta tttcaaaaag gttataaatt ttcagagaca gatttgttat
     9181 catattcaat agtactatta ggagcaacac tcagtgtatc taccttaata gacttaaaag
     9241 tttcaggaat tttatttata atattgcaaa tgacaataac aataatagct gcactttata
     9301 taggtaaaaa actaggattt gaagaaaact ttagatttct aatggcaagt ggaaatgcag
     9361 tatgtggttc ttctgcaata ggagcaacag ttccagtggt taatgcgaca gataaagaaa
     9421 agggaattgc agtaactatt gtaaatgtta ctggtatatt tcttatgttt ttacttccaa
     9481 taatatctca atttttatat agccatgaac ttgtaaaaac ttcagcaatg ataggtggaa
     9541 ctttgcaatc aattggtcag gttgtagcaa gtggagcaat tgttggagaa aatgtaaaag
     9601 atttagctac tatttttaag atagtaagag taatatttct agttgttgtt gtattagtat
     9661 ttggacatat gaaaaatcaa tctaataggg aaattcttga agaagaaaag gatgagataa
     9721 aaaagaaaaa agtgactata ccatggtatg taattggatt ctttataact tgtgcacttt
     9781 tttcaatgaa cattatacca caagaagtat ctgtgttgtg caaagaaata agtaacaaac
     9841 tagaaataat agctttagca gcaattggat taaaggttaa tgtaaaagat ttagtgaaac
     9901 aagggaaaga agtttcttta tatggtttat ttgtaggtat agtacaagtt gtatcagctg
     9961 tagttttaat aaaaatattt atatagcata aaaatagtat ttgtaatatt aatttgatta
    10021 attaaaataa tatgatacta ataatttata atataaataa aaaatgaatt tcgataagtt
    10081 taagtgggta taaacttata gaaattcatt ttttatttaa ggtaaaaatt taaagtaaaa
    10141 tattatttta cataataata accttataaa ttttgaaatt acttatcttt gtgttaaaat
    10201 aagtaaaatg cacatattag agctaagttt ttaagtaaat aaacaaaatg aaaatataaa
    10261 tattaaggag atttggtata tgaatgttta tgaaaaaggt aatggagtta tactagaagg
    10321 agtaacagat tttgacccag tacatatatt tgagtgtggt caatgtttta gatggcacaa
    10381 acaagaagat ggctcataca ctggagttgc aaaggggaga atattaaatg tcaaaaaaga
    10441 gaatgataaa atatatttaa ataatactaa tttaaaggag tttaacagca tttggtataa
    10501 ttactttgat ttaggaactg attatacaga aataaagaat aagctaaaaa atatggatga
    10561 atatttaaat aaggctactg aatttggatg gggaataaga atattaagac aagatggatg
    10621 ggaaatgctt atatcattta taatttcctc taataatagg attcctatga tacaaagagc
    10681 tatagaaaat ttatcaagaa aatttggtaa gtatataggt gaatatgaag gtaatgaata
    10741 ctatgcattc ccaacaccag aagaactaaa taaggcctca caagaggaaa taagagcatg
    10801 tcaaacaggt tttagagata agtacataaa aagtactaca caggcagtta tagagaataa
    10861 tgatgaggta tctgaatata caaatttaag tactgaggat tgtagaaaag agcttttaaa
    10921 atttaatgga gtaggaccaa aagtatgtga ttgtattgca ttatttggta tgcaaaaata
    10981 tgattctttt ccagttgatg tttgggttaa gagagttatg caagaatttt atatagatga
    11041 agacatgagt ttacctaaaa tgagaactta tggaatcgat aaatttaaag aaatgtctgg
    11101 atttgctcaa cagtacttat tttactatgc aagagaactc ggtataggaa aataaattaa
    11161 gggggagaaa ttatgggagt aataggaaca atatttctgt tctatttaat tatatcatat
    11221 ctagctggag ccataatttc tgtgattata ttgcttgaaa atagagaccc tgcaaaaaca
    11281 atgtcttggc tattgatgtt tattatattt ccaggtgtag gtttaatgat atatgctatt
    11341 tcaggtagaa atattagaaa aagaaaatta tttaagactc aaaaactagc taataatata
    11401 aaagaaaaga aactatttga tacattagaa aagataacag aaatagttga attagaaaaa
    11461 gaatctataa aacaaaataa gcttttaaga gatgaagagg atggtagtta tagaaaaagg
    11521 gtaataaata tgttgttaaa gactggtatg tttcccttta ctaaaaataa taaagtggat
    11581 gtatttgtag atggaaatga aaagtttaaa agacttatag aagatataag agaagccaaa
    11641 gaccatattc atttagagta ctttatcatc aaagatagtg aaataggtag ggtgttaaaa
    11701 gaagaactta taaaaaaggc taaagaaggc attaagataa gaattttata tgatgatgta
    11761 ggatgctgga gattttggtt taatagaaaa tttttccgag aaatgaggga agtaggtata
    11821 gaaattgcag catttttacc tacaaaattt cctataatag gagggaaatt aaactataga
    11881 aatcacagaa aaattgttgt tattgatgga ataattggat atacaggtgg aataaatata
    11941 ggagatgaat atttaggcaa aaatgataag tttggatatt ggagagatac tcatattagg
    12001 attaagggta tttctgtata catgcttcaa atgacatttc tgattgattg gtattatacg
    12061 acaaaagaag tattagtgac taaaaattat ttcccaagtg tagggaatgt tggtgaaagt
    12121 atgatacaag ttgttgcgag tggtccagac agtgactggg aagatattca ttatgcttat
    12181 ttttctgcaa tatgtcaggc tagaaaaaat gtttatatag aaactccata ttttattcca
    12241 gatgaaagtc tactaaaagc tataaagagt gctgctctta gtggagttga tgtgagaata
    12301 atatttccta aaattgcaga ccacaagata gtaaatatag catcttattc atattttgaa
    12361 gaaatattaa gagcaggtgg taaagtttat ttatataata aaggatttat acattctaaa
    12421 gtcgtaataa tagatgataa aattgcttct gcaggtacag ctaatatgga tttaagaagt
    12481 tttatgctta actttgaggt aaatgcattt atctatgatg aagaagttat tagggtaatg
    12541 acagatgatt tctttgagga tttaagtcac tgtgaagaac ttaatttgga ggtatttaag
    12601 aatagaaata tcatacaaaa aatcaaagaa tctgtagcaa gattattttc accaatatta
    12661 taaaaaatta ttaaaaaagg tattgaaaat tatgtgtgat tggtatatta taataattgt
    12721 tagcactcta ttagatagag tgataataaa taaaaagtat atatataaat ctacatatat
    12781 aagtagtatt aatttaaaaa taactaaaaa cataggcgaa ctagtctata cttaatattt
    12841 taggaggcgt gaataatgaa aataagacca ttagctgaca gagtagtaat taaaaaagta
    12901 gaagcagaag aaaaaactgc aagtggaata gttttaccag gagcagctaa agagcaacct
    12961 caaatagctg aagttgtaga agttggacca ggtggaatag ttgaagggaa agaaataaaa
    13021 atggaattga cagtgggaga taaggttata ttccaaaaat attctggaac agaagttaag
    13081 atagaaggac aagaatacac aatactaaga cagagtgatg tattagctgt tattgaataa
    13141 atatagaata aatttattag gaggggttta aaatggctaa agaaattaaa ttttcagagg
    13201 aaacaagaag agctttagaa gctggtgtaa ataaattagc agatacagta aaagtaacat
    13261 taggacctaa aggaagaaat gttatattag ataaaaaatt tggttctcca cttataacta
    13321 atgatggagt aactatagca aaagagatag aactagaaga tagatttgaa aatatgggtg
    13381 ctcaattagt taaagaagta gcaactaaaa ctaatgatgt agctggagat ggtactacaa
    13441 ctgctacagt tttagcacaa gctataataa gagaaggttt aaagaatgta acagcagggg
    13501 ctaacccaat acttttaaga aaaggaatac aaaaagcagt aacagtagca gtagaagaat
    13561 taaaaaatca atcaagaata gtagaaacac aagaggctat atctcaagtt gcttctatat
    13621 ctgctggtga tgaagaagtt ggaaaattaa tagcagaagc tatggaaata gtaggtaaag
    13681 atggagttat aactgttgaa gagtctcaaa ctatgaatac tgaattagat gctgttgaag
    13741 gtatgcagtt tgatagagga tttgtttctg catatatggt tacagatgta gataaaatgg
    13801 aagcagtttt aaatgaccca tatatattaa ttactgataa aaaaatatct aacatacaag
    13861 aattattacc agttcttgaa caaatagttc aacaaggtaa aaagttattg ataatagctg
    13921 aagatgtaga aggtgaagca ttatctacat tagtagtaaa taaattaaga ggaacatttg
    13981 atgtagtagc agttaaagct ccaggctttg gagatagaag aaaagaaatg cttcaagata
    14041 tagcaatact tacaggtgct caagtaatat cagaagaatt aggttatgat ttaaaagaag
    14101 ctgatttatc tatgctaggt agagcttcat ctgttaaggt aactaaagaa agtactacaa
    14161 tagtagatgg ttctggagat aaaaaagcta tagaagatag agtaactcaa attaaacatc
    14221 aagtagagca aactacttca gattttgata gagagaaatt aatggaaaga ttagctaaac
    14281 ttgctggagg agtagctgtt gtaaaagttg gagctgctac agaagtagag ttaaaagaaa
    14341 gaaagttaag aatagaagat gctcttaatg caactagagc agctgtagaa gaaggaatag
    14401 tagctggtgg aggaactgct tttgttagtg ttataccagc aataggaaca ctaattgaga
    14461 gtttagaagg agaagttaaa ctaggtgctc aaatagttaa aaaagcgtta gaagagccat
    14521 taagacaaat agctataaat gctggtcttg aaggtgctgt aattgtgcaa aatgttgtta
    14581 attctgaagc agaaactgga tttgacgcat tgaatgaaaa atatgtaaat atgatagaag
    14641 ctggtatagt tgacccaact aaagttagta gaagtgcttt acaaaatgct gcatcaatag
    14701 caagtacttt cttaactact gaagctgctg ttgctgacct tcctgaaaaa gaagatgtag
    14761 gaatgccagg aatgggtggc ggaatgcctg gtatgatgta atttaaaaaa tttaaaatat
    14821 aaattttaat ataaagaact atttcgactt agattttaaa ctctaagttg aaatagttct
    14881 tttgtatata tctaattttt gtatatctat aaattcaata cacatattta gataatcagt
    14941 atctagtttg taataatatt ttagagtagt attgttattt tttaggttat gattttagat
    15001 atatattaat taaggtataa ttgttgatag agaataaatc aaatttaaaa ggatgcgata
    15061 atatggactt aagaaaattg cttgaagaag ttaaaaatga aaatattgat atagattttg
    15121 cgttagaaaa gttaaaagat ttaccttatg aagaccttgg atatgcaaat atagaccatc
    15181 atagagaatt aagaaatggg tatccggaag ttatttattg tgaaggtaag agtgatgagc
    15241 atattttagg gattattgat aaaatgaatc aaaaaagttc taacatattg gggacaaggt
    15301 gcagaaaaga aacttttctg aaaattaaag aaatctataa tcatgctgag tacgaagagg
    15361 cgtctaaaat tcttaagatt caaaatcatg atatagagaa tataggaaaa ggtaaaattg
    15421 tgattgctac agggggaact tctgatatac cagtagcaga cgaagcttat catacagcta
    15481 agtttttagg taatgatgtt gatagaatat atgatgttgg agtggcaggt atacataggc
    15541 ttttaaataa aagacataaa atagatagtg ctagagtaat agtagccgtt gcaggtatgg
    15601 aaggtgctct tgcaagtgta attggtggac tggttgatgt tccagtaata gctgttccaa
    15661 cttctgttgg atatggagca aattttggag gattagcagc attactagct atgttaaata
    15721 gctgtgcatc tggaatatct gttgtaaata ttgataatgg atttggagca ggatatttag
    15781 catcaatgat aaataaactg taaatacatt gatataaatt aaacaaaact atataataaa
    15841 atttaatttc tattatattt ttactattaa aattgaggag acatatatga gtgaaaaaat
    15901 actttatttt gatataataa gcggtatatc aggggatatg acccttgcaa gtttattaaa
    15961 tttaggtgtg cctaaggaaa tttttttgga ggaaattcat aaactaagaa tgagcgatga
    16021 atttaatata aacatcagct cgaaaactga aaatggaata gtaggtacaa aagtagaagt
    16081 aattacaaaa gaaaaacata cacatagaaa tttagtggat atatttgagg ttatagataa
    16141 aagcactcta aacgaaaatg taaaatctaa agcaaaaaaa atatttatgg taattggaga
    16201 agcagaagca aaagttcatg gaactacaat agacaagata cattttcatg aggtaggggc
    16261 tatagattca attgtagata tagtaggagc ttctatatta gttgacttac tttgtgtaga
    16321 taaagtttgt gctagtacag tgtctgttgg ttcaggattt gtaaaatgtg aacatggaat
    16381 tatacctata cctgcacctg caactataga aattttaaaa ggtgtgccaa ttaaattaaa
    16441 taatgtaaat ggagaatgta caactccaac aggagctgca atcataaaag tcttgtgtga
    16501 tgagtttgta gatgagcttg aattttctcc aaagcaaatt ggatatggta taggtcataa
    16561 gaagtttgaa gtaccaaata tactcagaac tgtattaggt gaaaaaaaaa agaagaatta
    16621 atttatgaaa ttagtgcaaa cattgatgat atgtcttcag aaatctattc ttatttgtat
    16681 gaaaaaatat tagatgagaa agcattagat atttatacag agagcattta tatgaagaag
    16741 aatagaccag caacaaaagt atcaatttta tgtaatgaat cagatttaga aaaatttatt
    16801 aaaatattac ttttagaaac tagtactttt ggagtgagat ataatgcata taatagagaa
    16861 attctagaga gaaaatttgt aaaattagat acaaaatatg gagtggttac agtaaaacta
    16921 gggtattata aaggaaaact agtcaaggct actccagaat ataatgaatg taaaactata
    16981 gcaaaaaatg aagatattcc tataaaaaaa gtgtttgctg atataaattt tagaataaaa
    17041 gaagaattta gtgaaaactt attgacataa gtaataaatt ttgataaatt aatattcagc
    17101 cttattaaat aaaagtaaac agaaaaaaat atataaaata taaactcact cgtatatgct
    17161 cagaaatatg gtctgagagt ctctaccaag ataccgtaaa tatcttgact atgagtgaaa
    17221 ttattatacc aaaagaccta gttaagtaaa tgtgtaggct gagttaaact ccctatgtgt
    17281 tggctataag ataaaggtat tttggaagcc tgtatagtaa tttcactttt gctagaaatt
    17341 atggaagtta ctatacaggt tttttgtgta aaaagattta atataattta gactgtattg
    17401 tgttaaaata aatatataaa aactatatta tacaaggaga gatgtaaatt tatgaaacat
    17461 gaattagtac ttgtaataga ttttggagga caatataatc agttgatagc tagaagagtt
    17521 agagaaaaca acgtatattg tgaaatactt ccatgtactg caagcataga aagaataaaa
    17581 gaaaaaaatc ctaaagggat aatatttaca ggcggtccaa atagtgcgta tttagaagat
    17641 tctccaacaa tatcaaaaga aatatttgaa ctaggagtac caatacttgg aatatgctat
    17701 ggtattcaaa taatgtctca tgtattgggt ggagttgtaa gaaaaggtaa taaacaagaa
    17761 aaagaatatg gaaaaacagc tataacttat ggaaaatcat cattatttga aggaataact
    17821 acaaatagtg tatggatgag tcatacagat ttaatagaaa aagttccaga aggatttact
    17881 atagttgcaa atactaatga ttgtccagta gcagctatgg aaaatgtaga acgaaaccta
    17941 tatggagttc aattccatcc agaggtagaa cactgtttgg aaggggacaa gatacttaca
    18001 aacttcttat ataatatttg taaagtaaaa ggtgactgga ctacagattc attcatagaa
    18061 gataaaataa aagaattaaa agaaaaaata ggcgataaaa aagctttatg tgctttaagt
    18121 ggaggagttg attcttctgt tgctgcagtt cttattcata aagcaattgg ggacaactta
    18181 acttgtatat ttgttgacca tggtttactt agaaaaaatg aaggaaatga tgtagaaaga
    18241 atatttagag agaaatttga tataaattta ataagagtta atgcagaaga tagattctta
    18301 tctaaattaa aaggagtttc tgaaccagaa gctaagagaa aaataatagg tgaagaattt
    18361 ataagagtat ttgaagaaga atcaaataaa ttaggaaaaa tggatttctt agtacaagga
    18421 acaatctatc cagatgttat agaaagtgga catggaaatg ctgctactat aaagtctcat
    18481 cataatgttg gtggaatccc agaagatgta gacttccaag aaattgtaga accattaaga
    18541 gagttattta aggatgaagt tagaaaaata ggattagagc ttggtataga agaaggatta
    18601 atatttagac atcctttccc aggaccaggt ctaggaataa gagttatagg tgatgtaact
    18661 aaagaaaaat gtgatatatt aagagaagct gatgctgttt atatggacga gcttagaaaa
    18721 gcaggtttat acagagaaat atggcaagct tttgcaacat taccagatgt taagactgtt
    18781 ggagttatgg gtgatgaaag aacatatgct tatttagttg gtttaagagc agttacttct
    18841 tctgatggaa tgacttctga ctggtataag atgccttatg atgtattaga aagaatttct
    18901 aatagaataa taaatgaagt tgatggagta aatagagtag tttatgatat aacttctaaa
    18961 cctcctggaa ctattgaatg ggaataaaat atagaatgcc aaaaatccac ataaatacag
    19021 gatttgaggt ctctcttact tctatgttat actggtttga tactatcaat atttgccagt
    19081 atcttatgga ttaataagca aaaaacatcc tgctgaatga taaattcagc ggggtgtttt
    19141 tattttgttt tagaactcct ttgctctgaa actcgaagta tttaacactg atgattttgt
    19201 tatcttttag ttccttttgt gtcatgctct gaaagacaaa ttgatacaat aaaaaagtaa
    19261 tttcttaatc attgatggag atatgtgttt atgttactct tgatggtaat ttgagtaata
    19321 ttgttcatag ctttttatat tcacttaatt agaaaaccat ttggtataat cataaataaa
    19381 cttgatgata taatagaaaa gatttataat tattagaagt atataaaagt attttatgtc
    19441 tatgaaatgt atgtctataa aacatataga tgacttttgt atattaatgt atgaatattg
    19501 atattataga agaattgagt tgtcaatgat actaaatcaa atatttatag aagctggagg
    19561 gattatgaga aataaaataa ttataggaat tactttttca cttgtattac taatgtctgg
    19621 atgtgcttca aaacaaaccg cagaaataaa cgtattagca actactgatt tacatggagt
    19681 tataccttat gagttaactt cttatgtaaa agaagaaaga aaaaaagata aaaatctaac
    19741 tttagtagat gcaggagatt tttttgatag tggagaagtg tttggttcta gcatggatag
    19801 ttattttagt gctagatata aagatggtga taataacact gaaagttata ttgagacgcc
    19861 tatttcaaaa gatatgaaag aggtaggtta tgatgttgca gttcttggga atcatgaatt
    19921 tatatccaac aataaatttt atctagataa tatgatatct gattttgaga agcaaaaaat
    19981 aaatatttta tcggcaaata cgtataaaaa aaatggagat agttatacaa aaccatacat
    20041 aataaaaaat attaatacac ctgaaggaaa cgtaaaacta ggaattttag ggcttactat
    20101 aaaagaagtt ggtgagagga aacagtggaa agatggaaaa cttgttgact ctaagtcttt
    20161 agaactaaaa gaccaagagg gttacaatgg tgaattgtat atgaatgatt tagtagaaga
    20221 tgctaaaaag tgggttggta taatggaaaa agagaagcct gacattatag ttgctgtggc
    20281 acatacaggt gagaaaccta agaaacctaa aaacccagga aatagaatac aagacttagc
    20341 tcaacaagtt gatggaattg atgctatagt tgcaggtcat aatcatgttc aaattgagca
    20401 acatgattat aaaaataaat caggagaaaa tgttatagtt actgagccag gaaagcatgg
    20461 ggaatgtatt tctaagatta attttaaatt agaaaaaaac aatgataaat ggaatatagt
    20521 tgacaaatct agtaaattaa ttaaatttga aaaaaatcaa gaaagtgata aatttggaga
    20581 atttatttct tcaattaata aagtaagtaa agataagaaa gaaataagat tatcagaagt
    20641 aaagactcct gaatgggata aaatatatgt ttttaaatct ggaacatctg cagatgatat
    20701 atataaaaca ataggatata aatggagaaa tatatcatct ccaaagggtg atgacatggt
    20761 gcagattgtt gtaatgaata ataataagcc tgttagctat gtatatggtg aagctgaagt
    20821 tatgccaatt aatataaaat gtgatgaatc agagtataaa gataatgttg taactattta
    20881 tcctaataaa aatgataaat ttaaaattga aaaaggtaaa caagaatata gtattaattt
    20941 aaaacatata caatagaaac ttttatctta tttaagttta gattgtactt aaaataaagt
    21001 gtgtattatt ttaaaaattg atgataatta aaatcacaac attaaagaat agtacacact
    21061 ttttatgcta aaaaatagaa tgttaaaaaa ttaaaccttt aaattaaagt acattgctta
    21121 tattatccaa tattattttc aaaggttgtg taatattttt atccttatgt aatattgcta
    21181 acattttatt ttccatatct atcccatcta attcaatcaa tcgcaatttt ttatgtaaga
    21241 gtgaatctaa taataaattt tctggcaaaa tggttatacc taaccctgct tgtgcagctt
    21301 taataagtgc aaaagaatta acactttccc aaacaggata agctttttgg tttgaaagag
    21361 ataatacact gtccaaagta ttgcgaatgg cacttccttg ctcacgtaac aataacggaa
    21421 acgtgcataa ctgtcgtaga gataaggctt gtttagtatt ggtggtggtt atgcaatcat
    21481 acttttaatc caagagaaag tagtaaatca aactggatgg ataagtgaga aaatgtttac
    21541 agatattata actatttcac aaatgacacc agggccactg gctgttaata cttctacttt
    21601 tgttggactt caaattggag ggttttgggg agcagttgca gccactttag gttgtgtatt
    21661 atgtggtgta gtaatatctc tatttttata taattttttt caaaagcatc agaactctac
    21721 ttatatattt gaaatattaa atggtctaaa atcagcttca ttaggtttaa ttatctcagc
    21781 tgcggctaca attataatcc ttgcattatt tgggacgaat aaagtgtact tcaattctat
    21841 atttgagatg ctaaactgga ctgcactaat aatctttttt gttacactac tgatagtacg
    21901 taagtggaaa attaatccta ttgtgattat aataataagt ggtatactgg gaatattttt
    21961 ttatttataa gtttttggta agtagaggat atctattttt ttatatctta aatccaaata
    22021 gccaataaag taaggccatt tggattttta ataaattctg aaaagataat cagaaaccca
    22081 tataacattt aaattatatc actggttttc tacaaaccaa tcataagctt cttggcaaaa
    22141 tttctcacta tctatatagt aactattatg aacattatta atttcaacaa gtttcttttt
    22201 acattttagt gaattataat agctatcaaa atattgggtt ttaaaatatc tatcatgaag
    22261 tgttttaaag acaagagtag gtatgttgac tccatctttt atcttactat cttttgcaat
    22321 aaagcattct aacattgtat taattatctt tgtactattt ttccaaatat gaggattttt
    22381 cttttcaaat attttataaa attcattatc atagacttca ttataacctt tatatttagg
    22441 tggttttact gttatgtgaa aattaggaag aaacttaaag aaaaagttta ataatttaac
    22501 agtgagatag gtatagccta ctcctgttgt gtttttataa tcaacacaag caaactgagc
    22561 aaaacttttt aacttattgt ctgaacatgc ataatattgt gcaaacatac caccaattcc
    22621 cgtattccca tatagatgta tgttggaaga ataatttttt tctatgtatg taatgacaga
    22681 atctaagtct tttacaattg aattgcgaga aaaatctttt tcatatccat cacttttccc
    22741 tgttcctgaa aaatcaaaag caaatatatt tacattatgt ttggcaagtg gttcatatgc
    22801 tttttgcatc tcaagcgtag tgacaatagg agtatgtgta acaacaatat tcatggatga
    22861 ttttctggta ataaatgagt tacaaaaaat cattgtatct ccatttggta tttttatatg
    22921 tttccaactc atttgatttt cctttctcta ttataaaata taataatttt tatgttctgt
    22981 tttatatatt attcatattt tatcacagat aatatactaa agttaatttt tttattgtac
    23041 acctattata ataattcttc tataatataa gacattctat aattttatat ttaagttcat
    23101 gtgtatttaa ttgtttttat ataaaatgtc cagtaatact atttggattt atttttattt
    23161 catcaggagt tccagaagca acaatttccc ctccatttat tccactgtct ggacctatat
    23221 cgataatcca atcagaagca cgtataacct ctgtattgtg ttcaataatt aaaagtgaat
    23281 gctgttcatt tacaagtttt ctaaaagtaa ttaacaaatt ttctatatct ttaaaatgaa
    23341 gtccagtagt aggctcatcc aatatgtaca gcattcgttt tttagttttt gtccctaatt
    23401 cttttgctaa ttttatcctt tgagattctc ctccacttaa tcctaatgta ttttgtccaa
    23461 gctttagata tccaagtccc acttcatcta ataaggagag ttttctatat atatcagagc
    23521 aatcgctaaa aaatgttttt gcttctgcta cttccatgtc aagtatatct gcaattgtct
    23581 ttcccttgta ttgtatagac agtatttctt tttgatatct cttaccttga cattcactac
    23641 aagttaccca ttggtctgcc atatattgca gaataatttt tgttttacct aatccttgac
    23701 agtttgggca ttgccccttt ttgctattaa agctaaaata gctttcatcc aataattttt
    23761 gttttgcttg tggagtttca gcaaatagag aacgaatttc atcaaaaata ttgatatatg
    23821 tagcaggtgt agaacggcta gattttccaa taggtgtttg gtccattaag ataaaatcat
    23881 caatagcatc aaatccagta aattcagtat aatttttatc tgggatagat ttttgtttca
    23941 atttttcttc tagagcagga agtaaactgt gaaatacaag agagctctta ccagaaccac
    24001 taacacctgt aatagagcac atacagttta agggaatttc tacatcaata ttttttaagt
    24061 tatttgcatg gcatcctttt aagcttaacc atttacttgg tataatttta ttttgtgaac
    24121 ttattttatt gtatgtgctt aaatatttac ctgttataga gtttggattt tttataattt
    24181 cttgggttgt accttctgca ataatgaacc ccccttttgt accagcactt gggcctacat
    24241 caataatata atcagcagac agtattgtat ctttatcgtg ttccacaact ataacagtat
    24301 tacccttatc tcttagctct tctatcattt tgatgattaa attatggtct cttgggtgta
    24361 aaccaataga tggttcatct aaaatgtagg ttaagcctac taattcactt cctagctgac
    24421 ttgataatcg tactctctgc agttctcctc cagataaagt tggagctgtt ctatctgttg
    24481 atatatagct aagacctacc tttaatagat aactaactcg aatttttaat tctgttaaga
    24541 tatcatttac caaagagagc tcattttttt gaagttgatt tggtaattca cacaaccaat
    24601 tccataactg ttctatcgtc atttttgtta gttcagggaa gcgataacct ttgattgtag
    24661 tgaatctagc ctcaatgcac agcaattcgc caccacatgt attgcaagga attttgttca
    24721 tatattgatg caaggtatta ttttcagaac tagattctct aaaaagtctt tgaatgtggt
    24781 tgattgctcc tgaagcaggt cttctaatct ttgattctcg tccctttgat tcaaagctaa
    24841 attcatatat cttatcgtct gtaccatata agattgcatc tataaaatct cttggtaatt
    24901 cattccaagg tatatctagg tctatatttt tatcttttgc tattgcatat aattcaccta
    24961 ccatccaatt accatttggc tttttcccct tcaattttcc aaaatagtaa gtagctccat
    25021 ctaatattga aatagttggg tcttttacaa tcaattgagg attaacttga taggtaaaac
    25081 ctaatccatt acaatcgtga caagctccag tatgggagtt agcattgaag gttgaagtat
    25141 ttatcttaga aaaaatagtt tgacaatgtg gacaataatt ttttgtatta ttcttttttt
    25201 ctaatggaat tttacaatat ggacagtaac tttttccgat tgatacaaat aacattctta
    25261 aataatccca aatgcttgta agagtaccaa cagtagagcg aggattttgg cttccttttt
    25321 tctgttcgat agctatagta ggggttaatc cctgaagtaa atcaaattcc ggtttttgaa
    25381 gctgttctga aagttttgct ccattactta gattttcaaa ataacgtctt tttccttctt
    25441 cataaatcgt atcgaaagct aaagaagatt ttccactacc acttacacct gtaataacag
    25501 ttatttgatt ttttggaatt gtaacctcaa catcctttaa attgttttgc cttcctcctt
    25561 ttaaatagat agagctttta ttatgacaaa gtgaattttt aattgattta tttattgatt
    25621 ttgtaaattt gaatttcttt tcgagttcaa gaggattgcc taattctttt atttccgaaa
    25681 tgcctaatat ttttaactga gaaaagattg atttcaaata atcttcaggc aatttttcta
    25741 ttaacaaatt aaggtcttct atttttatat ttccgcttga taatatttct gccatttcat
    25801 ctaataaatt tttttctgct cttgttaatt gattgtattt tttggaactt aattctgtcc
    25861 ataaatccca tggaagcaat tctaaaccaa tcatattatt aaagtcatgt agaagattac
    25921 cctttacaga atgccaacct ttccaatttt tattatatcc aaagtcatct tgatgagcat
    25981 gtccttgccg acatcttatc caggcttctg aaccagttaa aaaaccatct gtttttccga
    26041 catcatgagt gtcaaaatta atttgaaaat aatttttttg tatgtcatct atttgtgcat
    26101 ctattcttat ccatctcttt gttagagtat catagtattc tacaagccag tggtcttcat
    26161 aagtaagctc actttcaaaa taattagcaa aacctgctct cattctagcc tcaaaacctc
    26221 gatatcttag gagcgataca agaagtaaag aataatctct gcacattcca acaagccttt
    26281 gttttgggtt tcttggaata gttagtgggt tagaatttat ttcaaatatt ctttttaaca
    26341 tttgaggaat tgtacgaagc aattcctcat cagattgttg tttattaaag gatataccat
    26401 ataattttcc ttggtctcca tgaattacta ggctttgaac cacttttaat aactcaattg
    26461 gagtctttgg taaatcttga tatttatttt caaatttttt aggatttgtg atgatgcttt
    26521 gatttttata gttatttatt ttcatttata tttcacctct ctaaatggat gatataccct
    26581 gacatggtgt catatgcaag ggcaatttaa attttttggc caaaatttaa aaataaataa
    26641 aaaatgatac agtgataaat tgtaatacaa atatagtttc aaattgcata taatataatt
    26701 atttaataaa accattatct atggaatgtt aatgaaatgt taagatggca ttcttgaata
    26761 ttaagaaata gaatactata atatagtgtg aaggattttt agtaaaaaaa tttgtaagtg
    26821 ttaatataca ttaaaaaata gatttataat ttggaggaaa aaaatgaaat tatcaaggta
    26881 taagaaaaat agtttagttc tgtctataat tttatgttca cttattattt caatccttgt
    26941 aattactgtt tttaataccg taaaactaca caatatatct aataaacaat ctaaaaacta
    27001 tttaaatgat gtatctacac agattgtaat gaatgttgat tctaaaatca aatttatttt
    27061 atccgactta agaataatgg cagattttat taagcaatat gaaggtgata gtcgatatga
    27121 gtatttatct aaaagaaaga ctagttataa gtattatgat gttggaataa tagatttaga
    27181 aggtaaagct gagtttttaa gtggaaaaaa gtttgactta aagaatactg tttcctataa
    27241 aaaaggaatt gaaggtaaag aatactcaga agtaatagag tcaatgggtt ttgttcttta
    27301 tagtgtacct atatttaatg ataatcatga agtatctagt attttaatag gtgtatccaa
    27361 taaagaaagt atgaatgaga ttcttaatat agataacttt aatggaaaag gaactattga
    27421 aattattaat agtaagggaa aacctctttt cataggaaaa aatactgaat taataaagga
    27481 ccttagccat aaatataaga gttctggaga tgaaccttgg gcacaaaaga tgcttgagga
    27541 ttttaaaaat gataaatcag ggaatattac aataacctca tcaaaaggtg tacaatgctt
    27601 attaacttat catccaatta caagaggttt aaatgattgg cattttcttc tcatagtacc
    27661 tgaagatgct gtacttggtg aacttaataa attaaactct tttacaataa caatgacatt
    27721 tataattaca tgtattattg gaattataac attaatttta tatattataa gaagaaaata
    27781 tgtaaatcaa atagaaaata ttgcatattt agatagtata acaaatggta taaattcaac
    27841 taagtttagt atgctagtca aacctttaat ttcagaatca cctgatagta cttatatgat
    27901 gatagcaatg aatataaaag attttaagct aataaatgat tgtttcggta gtgaaaaggg
    27961 aaatatgaca ctaaaacatc tttacaatat tttgtataaa aacattgatg aaaatgaaga
    28021 gtttgtgtgc cgtcatgatg cagacctttt ctatttgctt attaagaata gaccagtgtt
    28081 agaagccctg gagttattat ataaaataga agctgacaca aattatttta ataaaaatag
    28141 ggaaaatcca tactttttaa gactatcagt aggtatttac acaattgaaa atcataatga
    28201 agatttaatt acaatacaag accatgcaaa tacagcaaga aaaagcttta ataaaactca
    28261 tcaaagtgat ttttcttttt atagtgatat agaaagaaaa cgattaatat ctgaaaagga
    28321 aatttcaaat ttaatggaaa aagcccttga aaataaagaa ttttttatgt gcttacaacc
    28381 taaaattgat ataaaaagtg gaaaaattag tggagcagag tctcttgtgc gttggaaaaa
    28441 ttcagaaaag ggcataattt atccaagtga ttttattcct cttttcgaaa aaagtggatt
    28501 tatatgtaag ttagatttat atatactaga agaaacatgc aaattgattt ctaagtggat
    28561 aaaagaaggt aaagaagtgt tgagaatttc tgttaatgta tcacgtcaac atctgaatga
    28621 taaattgttt ttagaaaaat ataagcatat atgcaataaa tacaatgtac caacatgtct
    28681 gatagattta gaacttactg aatctatttt cttagaaaat ccagaagcta tagatataat
    28741 agaggatatt cattctaatg gatttaaatg ttctatagac gattttggat ttgggtactc
    28801 atcattaggg atattaaagg attttaaagt tgatataatt aagttagacc gttcattctt
    28861 tgttagcaaa aataatatag accgtggtaa ggtagttatt aagtctatta ttgaacttag
    28921 caaaaggcta ggtatgaaag taactgctga aggaattgaa gaattagaac aagttgagtt
    28981 cctaaaaaat gtgggatgtg attatattca aggttatgtt ttttcaaaac cattattaat
    29041 ttctgacttt gaaaattttg catatgaaaa taataaaatt aaaaatatcc attattaaat
    29101 gtacaagata aaaaatttag gcaagttata taattaaaaa tataacttgc cttttttatt
    29161 tatatattta ataaaaatat atttgcagat tttgtaaaaa taattttcaa atgttgtaag
    29221 ttgtggctaa agctttattg tattattctg aatatagaaa gatagttact gtcttagata
    29281 aaaactgaaa catacataat aatataaaat ttgctatatt aataactcta attattttgg
    29341 aaggagggaa aatatgataa gtgggagaat gttactcatc atgaattttc taaaaggaaa
    29401 aaataagaca agttacaagg atattagcaa acaacttagt atagaagaaa gaaaagttag
    29461 atatgatata aataatttaa atattgtttt gcgttcatta aacaagtcag taatacaaaa
    29521 gatgaacaaa ggtgtgttaa ttattcctga tgacttccaa gtagtatgta taaataataa
    29581 cgaatatgta ttttccttaa ctgagcgagt atctattatg aaaattatat caatgtttaa
    29641 aatagataat ctaaacttag aaaaattaag taaagaattt caggtttcta gaagttcaat
    29701 taaaaatgac cttaatgttt tgtcaagtga attagaaaga aaccaaataa ctattactta
    29761 tgataaatct tttaaaataa gtggagaaga agacattata tataagcaaa gattgaacat
    29821 attaaaatcg tattcttatc tttttggtaa ggaaaaatct gatttaagtg tatttgagaa
    29881 gtatttagtg aatgtaattg aaaaaatatt caaagggatt tatttgattg atatatataa
    29941 ttggtctatg aatttattat tgcaaatggg atggacttta aatgatgaat cttttacatg
    30001 gtatgtgtca aacattttcc tttttacatg gtatatacat tcagatgaaa aacatccttt
    30061 agaatctgct tcattaagtg agccgtgttt tgaaaataag tttgaccaag aatttgaaac
    30121 aattattcaa aaaaagttga ctaaggaaca aatttttctg ttaaagagtt ttgtattttt
    30181 tacaaataaa tatgcaagtt taaacgagga tatggatttg attagtacag aaattatagt
    30241 tcaagaccta atttacaata tgtcagaaat attagagatt agctttaaag aagatatgat
    30301 tctttataaa ggattactca atcatattgc cccattgata gagagaatca agcagaatat
    30361 acagatttat gaggaattac ttcatgtaat accagataaa tatcaatatg tacttgaagc
    30421 tactaaggtt tctattcaaa aaaacagagt tttaagtcaa ataaagaatg aaaatgaaac
    30481 aatacttttg gcaatacatt tcttaggaag tatacataga aatgagagga ataactacaa
    30541 aaatatattg ttagtttgtg gatttggata tggtgtaatt gctatgttaa aggatagttt
    30601 agtaaatgat taccagataa atatagtaga gagtattcca tcatataagt tacaagatta
    30661 taaagactgg gaaagtattg acttcattat aacaacatca aagatagtaa tagacttgcc
    30721 taaaacaatt attgagataa atccattttt acaagaagag gattatttga agtttgaaga
    30781 aaaagggata aaaagaaaga atgtcttaac taattatatg tcaataaaaa aaaggttgga
    30841 ctttttagaa gaagatgccc aaaaaagagt attaagtatt atatgtcaag aacttggata
    30901 cagtgatgag agaatattat ttagacaact taatttaagt gatttaatag ggattgatac
    30961 cataaggata attgatggag atatgaaatg ggaagatgct gtaaaattta gttcaaacat
    31021 acttgctaat tgtgattttg tatcagcaca atatgctgag aatattatag atattttaaa
    31081 aaatgtgggt ttttatgctg taaaagataa cgaatttgct ttactacatg gcaatgatag
    31141 ttcattagtc aaagtaagtt cgataagttt attaatatgt aaagaagcag ttagttttgg
    31201 agataaaaag gttaaaatca tattttgcct agcaagcaga gataaaaaag agcaaattcc
    31261 tgcaataatt aatctaacta aaatggttta taagactaat tttattgcta tgttagaaag
    31321 tgcaaagaca aaagaagaag cagaagtttt aattcataaa tatgagaagg aggtcactgt
    31381 atgaataaat tagttaatgt aaaatgttca tttaaaatac atgttaattc aaaagaagaa
    31441 gccattatat cgttagttga tgtagtgtca aaagaaggat atttaataga taaaaatcaa
    31501 tttttaaaag atgttttaaa gagagaagaa acattgtcga cttatattgg acatggtata
    31561 ggtcttcctc atagtcaaag tgtaggtgtt aaaaactctt gtattacaat aggaaagctt
    31621 gatactccta ttgaatggac agaagaagga gaaaaagtgg atttaatatt tttgatatca
    31681 gtaacaaaag acaatgaaaa taatcttcat ttaaagattt tatcaaaatt agcacgttta
    31741 ttgatgcatg aaagttttag aaatcaaatt agatgttcag atgaacaaac tgtttataat
    31801 cttataaaag aaaaaataga ggaggaagat taaaatgaga attttaaagt ttttgcaaaa
    31861 acatcttatg acagcaacat cttatatgat tccatttgtg gttgcaggag gaatattatt
    31921 tgctttatca gtaactttaa gtggacaagc tgctgttcct gaaacaggct ggttggctaa
    31981 actgaatcaa attggagctg ctggcttggc tttattcatt cctatattag gtggatatat
    32041 agcatttagt atggcagata aaccaggatt agctccaggt atgataggtg catatttagc
    32101 aaaagaagta aatgcaggtt ttataggtgg tatcatagca ggatttatag caggatttgt
    32161 tgttttacaa ttaaagaaaa ttaaattagc tcctacaatg agaactttag gttctatatt
    32221 tatttatcct ttagttggta cattgattac aggtggtata attgtatttt taattggaga
    32281 acctatagct tcttttatga catggatgac aaactggtta aatggaatgt ctggtgtttc
    32341 taaaattcct ttaggtggaa tcttaggagg aatgatagcc tcagatatgg gtggccctat
    32401 aaataaggtt gctgctactt ttgcccaaac tcaagttgac acacttccat atttaatggg
    32461 tggagttggc gttgcaattt gtatacctcc aattggttta ggattagcta cattattgtt
    32521 tccaaaaaaa tttagcaagg aagaaagaga ttcaggtaaa gcttcactat taatgggatg
    32581 tgttggaatt acagaaggag ctattccatt tgcaacatca gatccagtta gagtaatacc
    32641 ttgtattatg gctgggtcga ttgtaggaaa tattatggct tttttattag gatgtttaaa
    32701 tcatgcaccc tggggaggtc tgatagtgtt acctgtagtt gacaataggt tggggtatat
    32761 tgcatcagta ttaactggag cagtagttgt tgcggtatta atgaaattag taaaaaaaga
    32821 cgtgaaagaa gatgaagaaa ttgaagaaga tttagatgaa tcaattgaat taatatttga
    32881 agaattataa atataaatgg aaggaatttg tataaaaaag taaatataaa actataaatt
    32941 taaaggagat aagaatatga aaattgtagc agtaacatct tgtcctagtg gagtagcaca
    33001 tacttacatg tcggctgaat cacttgaatt atcagccaaa aaatttggta tagaaataaa
    33061 ggttgagact caaggaagct ctggaataga taatgaattg tcacttaaag atattgatga
    33121 agcaacttgt gtaatattga ctaatgatgt tgagattaga aatatggaac gttttaaagg
    33181 aaagaaggta gtaagaatga gtgtatctga gataattaag aaatctgatg cattaattaa
    33241 gaaaataaaa gatacttttc aataaaaatt gatttatttt acatataact caaagatgtt
    33301 tttaaagaaa tttttattaa aagcatcttt gaaaatatct taaaaataga ataaaaggag
    33361 atatagtcat gaaaaagtta ccaataaaga ctgtcgtgca aagattacta aacttacaag
    33421 aagaggggaa aagtgcaact cttttaggaa ttggtcctat gtcaccaaac ttgttacaag
    33481 caagttttga actagcaaaa gatgatgatt ttcctttaat gtttatcgca agtagaaatc
    33541 aagtagatgc agatgaacta ggtggcggtt atgtaaatgg ttggaatcag gaaactttta
    33601 caaaagatat taagaaggtt gccgacaaag ttggatttga tggtttatat tatttatgta
    33661 gagaccatgg tgggccatgg caaagagata aagagagaaa tgaccatttg cctgttgatg
    33721 aagcaatgga gttaggaaaa aaatcttatt tagcggacat tgaagcaggt tttgacctat
    33781 tgatgattga ccctacaaaa gacccatttg aaattggaaa agtaattcca ctagatgtag
    33841 tactagaaag aactgtagaa ttaattgagt attgtgaaaa cgagagaaaa agacttaatc
    33901 taccagacat aggttatgag gttggtacag aggaaaccaa tggaggtctg acttcaactg
    33961 agacgtatga aacatttata actagactaa aggttgaact cgataataga ggtttgccaa
    34021 tgccaacatt tatagttggt caaactggta ctttaactag aaagacagaa caagtaggaa
    34081 cttttaattt tagaaatgct tatgatttag ctcaaatggc taagaaatat ggagttggtt
    34141 taaaagagca taatggcgat tacttagatg atgtaacttt attagaacat ataccatctc
    34201 aaattattgc tacaaatgta gctcctcaat atggtactga agaaactagg gcatatttaa
    34261 aattagcaga agttgagtgt aaattagaaa aagagggctt agttgagaaa acttctaata
    34321 tcagacatgt tcttcttgtt aatgcaattg aatgtggacg ttggagaaaa tgggtagtag
    34381 gagaacaaaa aaatctaact actgaagaga tatttaagga tgaagtttta tctaatgaaa
    34441 tactagatat tgcaggacat tatacattta ataatgatga tgttaaaaaa gaaatagaag
    34501 tgctttatga taatttaagt aagaataata ttgatggtca aagatttgtt gtagaccata
    34561 ttaaacgtcc tttgcgtgat tatgcagaat gctataactt aaaaggtgtt actacgagga
    34621 ttttaaataa ataataggac ttatagtggt gagtattgtt atactaggta tttatagtta
    34681 taattatact taccactatt aaatttagga ggactaatat tttgattaat ttaatttttg
    34741 atgtagatga tactttatat aatcaattaa cgccttttta cactgcatat aataaagtgt
    34801 tttcttcaat aaaggatatt tctattgaag atttatatat gagtagtaga aagtatagtg
    34861 atgaggtatt tcatatgact gaaaatggag aaatgccaat caaagaaatg catatttata
    34921 ggataatgaa agcttttgag gagttgggaa attctattac agaaaaagat gcacaaagtt
    34981 ttcaagatga gtatatatat caacaatcac aaattacact aataccagaa gttgagagga
    35041 ttttaaattt ttctaaagaa agaaatatta atctaggaat tataactaat ggcccatcaa
    35101 accatcaaag aatgaagtta aagcagttaa atattgaaaa ctgggttgat aaaagtaata
    35161 tttttatttc tagtgaggtt ggattttcta agccagatac taatattttt agagtagctg
    35221 agaatgttat gaatttagat agagaaaata catattatgt gggtgattct tatagaaatg
    35281 atgtattagg tgctaaaaaa gctggttgga agagtatatg gttgaaccat agaggacatg
    35341 aggtagaaga gttgttttac aaacctgatt ttgtaatttt ggaacataag gatttgatat
    35401 ctttatttat aaaaatatgt agttataaaa atatgtagta aaaaaatgtt aataatagaa
    35461 ttgtataaaa taattaataa taactatata aaattatatg tttagattgt gagcaatcta
    35521 ttttatgtta aaaatagatt atttagaaga aggggaaatc ttaaaaaaat ttgaatatcc
    35581 cctttttatt atttaattaa aatgaaatag ttagaaatat attttattta gtttagtcaa
    35641 taacattttg ggattcttta taactacatg ttaactgttg aataggttta tcatatggtt
    35701 taacccatcc tttattaata cttaattctg taagtgctgt gtgaccttct accatttgag
    35761 ttaatgaagc tgaatatata gctcttaatt caggtgtact acttgtcaat gcagaattaa
    35821 gatacatatt ggcagatgct ttagcagatg aaagcatact taacattatt actttatcgt
    35881 ctatataagt attgttctta attagatttt ctatcatatt tctcatgtta tctatacctc
    35941 ctcagaaatt agtatttcat tttcaactat aaattgttgc atagctttaa ttcttccttc
    36001 tgttgctaat atactagatt ctgattgtct ttttaggtct tcatctgata ttaacatatt
    36061 tattgctctt tgcataagta atccgtcaga ctccattttt ataactgctg ctaaagatat
    36121 tttttcagtt tctgaaagta ttctcattta atcatctcct atattgtaat ttatgcttta
    36181 atagtatgtc tttcttttca aggaaataac cattacactt ttttacagta ttttatattc
    36241 tctttatatc aaaatttagt cgacctaata tattaataat aattttgtag gatatttatc
    36301 attaaatatc tataaaataa attatttgaa tattcataaa gcaaaattta tttgtatgta
    36361 cttcttcaag agtcaatatg agttttttca aaaaattaat ttttatgata atcattaagg
    36421 gctatatttt tcttaaagtt gtgattatca ttattaattt acaataaaat tgccgaaaag
    36481 ccctctcctt taggtgtagg gatgaaaggt aaggtcttaa tataaaaaaa gttagaataa
    36541 tgcttgtaat ctaataccgc tactgatacc ctatatttag gaggtgttag tttttatgta
    36601 taaatctaac aaaaatatag tttatgattg caaatatcat attgtatttt gccctaagta
    36661 tcgtaaacct gttttagttg gcgaagttga aaagatgtta aaagaaatac taccatataa
    36721 agcagatgaa cttggagcag aaataataga aatggaaact gataaagaac atgtgcactt
    36781 attaatatct tgtgaccctc aatatggaat tcataaagta gtaaaaggtt taaaaggctt
    36841 tagttcgaga gtattaagag agaactttcc acatttaaag tctagtatgc ctagcatgtg
    36901 gactaatagc tattttgtag gtactgttgg ttctgtgagt ttagaagtag ttaaacaata
    36961 tatagaaaat caacaagtta ggacaaagaa atgataaaaa ctcatagagt gaaattaaac
    37021 ctaactagaa cacaatttga attagtaagg gaaaagcaaa tggaaagtgc taactgttgg
    37081 aactacatag ttaacttatc taaagaatac tattttgaac ataaacaatg gattaaaaag
    37141 aatgatatac aaaagttaat caaaggtaag tacaatcttc atagtcaaac tattcaagct
    37201 atatcagata aatttgatgc aaatagaaag actatatcag aattaagaaa aaaaggtaat
    37261 actaaagcaa aatatccata caagactaag aaattttata taataccttt taaagctagt
    37321 gctattaata gaaatgctaa aggcaactta aaattatcaa tgtcaaaagg caggtattta
    37381 gaattagatt ttaatgttga gaatataaaa actgctgaaa tagtatggcg aaatggatat
    37441 tatttatact acacttttga caatgaatta aatggagttg tagccaaagg cactaatacc
    37501 gtaggtgtag acttagggga aatacattcg atagctagtg taacaaatga aggtgtaggg
    37561 cttattctat ctaatagaga aggcagaagt attaaacaat ttagaaataa gatgtatgct
    37621 tatatatcta aaagattaag taaatgtaaa aaaggtagta gacaatcaaa gaagttatgg
    37681 agattaaaaa ataagataag aagtaaaaca gataatcagt taatgaattt atatcatcaa
    37741 acaactagaa agtttataga cttttgtgta gagcagaagg tatgtgaaat agttcttggg
    37801 gatataaaag gggttgaaaa agatactaag aaaaagaaaa gattaaacag agttaataga
    37861 cagaaaatat ctcaaatgga gtatggtcga ataaaggact atataaaata taaagcaaaa
    37921 gagcaaggta ttgaagttaa gttagttaaa gaaaactaca cttctcaaac ttgcccaaag
    37981 tgttctaaaa aacacaaacc aactggtaga acttatagtt gtacttgtgg ttatgaaact
    38041 catagagata tagttggtgc ttggaatatt ttaaataaaa agcataaata tggtttagta
    38101 gattttagaa taaatcataa gcaaccaata aatttaaaag tatctaccgt ttgagccatg
    38161 tgtgggcgaa taactttaag gtagtagtgg cgaagacacc gtccgaagta gcttggcttt
    38221 gtaaccacct ctgttgtact gtttaagtat aaataagtgt gttagccacc aagaatcccc
    38281 ttgctttagc tatggggagt gtgtcaataa aatactttaa tgaaaaatag ctaactttaa
    38341 ttgtaggata ttcttaatat atttgaaata aataaaaata ttgaaggata taatattcaa
    38401 ttggtatata aataataaat gatagttaaa ttttacctta gaggctaatt aggtgtttag
    38461 tcaaagctaa tgcctttaga atacaaatgt attctaaagg catttttact atgtattttc
    38521 atttatatta ctaaacatta tctattttta tatacatttc tatgtatttt attttcatca
    38581 aatcggtcta taaactcatt tttttcttca ctatatccaa tagatataag tgcataagga
    38641 actaatttta atggtaagtc taaagtttgt ctaactttat ttaccacttt atttttagga
    38701 aatactcctg cccaacaagc agctagacct tgattagtaa cttctaataa taaattttgt
    38761 gtgcaagcac ctaaatcttg tatccattta ccaggtttta agaatttatc tctatttcca
    38821 agcactacta tacataatgg tgcaaattct atatgctttg ctctatgctg tgactttgag
    38881 agctgtttca gcagttcctt atcatctact ataataaatt cccaaggttg agcatttttt
    38941 gatgaaggtg cttgcatagc tgattttagt atttcagtta ttttatcttc ttctatattt
    39001 ttatctatat atgttcttat tgaacgtcta ttttttataa cttctaacat acagattctc
    39061 ctttaaataa aattatttct atttatataa tatcattact ttaaatttta gctattactt
    39121 atataaagaa taaactttgt gttggtttta tctaagtaaa agtaacattc agaactaaaa
    39181 aacaagacta taaatccaaa gtattaatta aaaaagaagt aataaaaaat aatatacaag
    39241 atttaaaaaa gagtattggg cttaaaattt aattagaaaa ccaattttga gataatctgt
    39301 tttttatttt aacaaaaaaa cacaaataaa gttcgatacg tagcgaatga aaaaattaca
    39361 agaaatattt tttttaaaaa atttcaaaaa agagttgata atatatgtat attaatataa
    39421 aatttaaggt gtataattcg tataataata cgaatgattt gtagtgtaaa ataacttata
    39481 aacggagagg ggaagtctaa tgaaagtagc agtagttatg ggttcaaaat cagattatcc
    39541 aaaattagaa gaagggataa aactgcttga aaaatatgga atagaagttg tagcaagagc
    39601 attatcagct catagaacac cagaacaact ttcaatattc ctaaaagaaa tagaagatga
    39661 tactgatgta ataatagcag cagcaggaaa agcagcacac ttaccaggtg taatagcttc
    39721 acaaacatta attcctgtaa taggattacc tattaaatca tcaactatgg atggactaga
    39781 ctcactttta tcaatagttc aaatgccaaa aggaatccca gttgcaacag ttacaataga
    39841 cttaggttta aacgcagctt tattggcatt acaaataatg actttaaaat acccaaaatt
    39901 aaaagaggat ttgaaatcat atagagaaga gatggcacaa aaggtgttag aagacgacaa
    39961 gaatttaagg gggtagcaga aatgttatta tatgaaggaa aagcaaaaca agtatattct
    40021 acagataatg aaaatgaata tgttgtttat tataaagatg atgcaacagc atttaatgga
    40081 gaaaaaaaag cagaaatatc ttcaaaaggt atactaaata ataagatatc aacaataatc
    40141 tttgaaatgt taaaagaaaa taatataaat actcacttta taaaaagttt atcagataga
    40201 gaaatgttag ttaaaaaagt agagatttta ccattagaag taatagtaag aaatattgct
    40261 gctggttcta tctgtaaaag agttggtctt gaagaaggtg tagttttcga tgagccaatt
    40321 tttgaaataa gctacaaaaa tgatgcttat ggtgacccaa tgctaaatga tgattatgca
    40381 gtggcaatga agttagcaac tagagaagag ttaaaattct taagagaaga aactttaaaa
    40441 ataaatgagt tactaaaagc atttttctta aaattaaacc taaagttagt tgactttaaa
    40501 atagaatttg gtaaggattc tgaaggaaat ataatattgg cagatgaagt atcaccagac
    40561 acttgtagac tttgggatgt aaatacaaat gaaaagttag ataaggacag atttagaaaa
    40621 gaccttggag accttgtaga aggttatacg gaagtacttt caagaatgaa taataaatag
    40681 gaggcaaatg tatgtgtgga gttttaggga tttactctaa taaagatgta actaaagaac
    40741 tatattattc tttatattct atgcaacaca gggggcaaga aagctgtggt ttagctcttt
    40801 tagatgatgg agaaattaag tacaaaaaag atatgggatt agttggagat gtttttaagg
    40861 aaaatgaatt atctaagtta aaaggaaata tagggattgg acatgttaga tattcaacag
    40921 ctggaggaag tcatgtttct aactgtcaac ctttagttgg aagttgtaga aaaagacaat
    40981 tagccatagc tcataatgga aatctagtta atgcaaatta tttaaaagat atgctagaag
    41041 aagatggata tatgttccaa actaattcag atacagaagt gattttatat atacttgcta
    41101 gatattacaa aggggatata gtagaaagtt taaaagtgac aatggattat ataaaaggtg
    41161 catatgctct tgtaataatg agtcaagaag aattggttgc agttagagac ccacatggat
    41221 tcagacctct tgtacttggt aaaaagggtg atgaatatat atttgcatca gaaaattgtg
    41281 caatagatat acttggcgga gaagttataa gagatgtaga gccaggagag ataatagtag
    41341 tcaaagatgg agaactaaaa tcatattttt attcagaaaa ttataaacca gttaagaaaa
    41401 gttgtatatt tgaacacata tactttgcaa gaaatgatgc aactatagac aatgtaaatg
    41461 catatgaatt tagaattaaa tgtggtgaga gattagctca gaatgaaact gtaaaggcag
    41521 atatggtagt tccagttcca gattcaggtt ggccaggagc tattggttat gcaaatgcta
    41581 gtggattaaa aataagtgaa ggcttagtta aaaatagata tgtaggaaga acatttataa
    41641 aacctacaca agaagaaaga gaaatagcag taaaaataaa attaaatcct ctttcaacaa
    41701 taataaaagg aaaatctata attttagtag acgactcaat tgtaagagga actacatcta
    41761 aacaattagt aaaatcttta agagaagcag gagcaaaaga aattcatctt agaataactt
    41821 ctcctccagt agcttactct tgttactatg gaatagatac tccaaatcgc tctaaattaa
    41881 ttgcatcaag taacaacgta gaagaaatga gagaatatat tggatgtgat agtttaaaat
    41941 tcctagatat agaaggaatg ttagatgcaa cagaacataa gtctactttc tgtaaagctt
    42001 gttttgatgg agaataccca gttaagaaaa ttgataagga ggaactactt tcatgttaac
    42061 atataaagaa tcaggtgtag atatagatga aggaaataga gcagtagacc ttataaaagg
    42121 taaaataaaa ggaacttatg atggaaatgt agttggagat ttaggtaact tcagtggatt
    42181 atacagttta aaagattttg taggtatgaa agaaccagtt ttgcttgcat ctacagatgg
    42241 tgttgggact aagttaaaaa tagctcagat gatggacaag catgatactg ttggaataga
    42301 tttagttgca atgtgcgtga atgatttaat ttgtcaagga gcaaaaccat tattcttcct
    42361 tgactatata gctcttggga aattagtgcc tgagcatatc gaaaaaatag ttggtggaat
    42421 tgcagatggt tgtaaaatgt ctggttgtgc actaattggt ggagaaactg ctgaaatgcc
    42481 tggaatgtat ggagaagatg actatgattt agcaggattt tctgtaggaa tagcagacaa
    42541 agaaaagatt gtttctggaa acaatgttaa atcaggagat gtattggttg gtatatcttc
    42601 aagtggagta cacagcaatg gattttcttt tataagaaag atatttttag aaacttacaa
    42661 ttataaaatg gaacaatatg ttgaagaact aggaatgaca gttggagaag cacttttaac
    42721 accaactaaa atatatgtta aattagcttt agatgtatta gctaaacatg acataaaagc
    42781 tatagctcac ataactggtg gagggcttat agaaaatata acaagagtta tacctaaagg
    42841 gttaggacta gatatcaata aaaaatcttg ggagaagcct cctatattta aaatgataga
    42901 aggttttaat gctgttgatg agagagaact tcataagagt tttaacatgg gtattgggct
    42961 agttttaata gtagataaag aaaatgctga tgatgttgta aactttataa ataatagaga
    43021 aaatgacaat gctgcttatg tagataaaaa atatagtgaa ttattagaag ataaagctta
    43081 tataattggt gaagtagtag atagtcatga aggtgtagaa ttatgctaaa tataggagtt
    43141 ttaatatctg gtggaggaac aaatttacaa gcagttatag atggtactga gtctggagaa
    43201 ataaaaggtc aggttaaagt agttatctca agtaagcaag gtgcttatgg gctagagaga
    43261 gctaaaaatc ataacataaa agcaatttgt gaaactgatg aagacaagat aatagaaatt
    43321 cttaaggaaa ataaaataga tttagtagtt ttggctggtt atttaaaaat aatcagccca
    43381 aagctggtta acgagtttag aaacaaaatg ataaacatac atccatcatt aattccttca
    43441 ttttgtggag ctggattcta tggagaaaaa gttcatcaag gtgttataga ttatggagct
    43501 aaagtaactg gtgctacagt tcattttgta gatgaaggtg ctgatactgg accaatcata
    43561 atgcaagatg tagttaaggt aaatcaagat gatgatgcaa agacacttgc aaaaagagtg
    43621 ttagaagttg aacataggat tttaaaagaa agtataagtc ttttttgtga aaataaatta
    43681 aaacttcaag gtaggagagt gtttataaat gagtaagaga gctttaataa gtgtaactga
    43741 caagactggt gtagtggaat ttgctaaaga gttaaataaa ttagattatg aaattatatc
    43801 tacaggaaat acatttaaaa cattaaaaga aaatggtgtt aatgtaatgc aagttgaaga
    43861 tgttacaaat tttccagaaa tattagatgg aagagtaaaa acattaaatc catatataca
    43921 tggtggtata ctttataaaa gagataaaga atctcatgta gaaactgtaa atgaacacaa
    43981 aatacattca atagatttag tagctgtaaa tctatatgat tttgaaggaa ctttaaaagc
    44041 tggaaaaagt catgatgaaa taattgaaaa tatagatatt ggtggaccat ctatgatacg
    44101 ttctgctgcc aaaaactata aggatgtaat agtagtagta gatataaaag attatgattc
    44161 tataatagaa aaattaaaaa cagatacaat gacattagaa gacagaaaaa aattatcata
    44221 taaagctttc tcaactacag gaagatatga tgcgttaata tctagttatt ttgctggaga
    44281 agttggagat acgtatcctg acatattaaa tttaactttc caaaaagaac aaactttaag
    44341 atatggagaa aatccacatc aaaatggttt cttatattca cagtcaaatg caaaaaatcc
    44401 aatcttaaat tatgagcaat taggcggtaa agaactttct tttaacaact taaatgattt
    44461 acatggatgt ttagaagtaa tgagagaatt taaagatagt gaagaagtag tttcagtagc
    44521 tataaaacat gctaattcat gtggggtagg tttaggaaaa gatgcttttg aagcatatac
    44581 aaaatgttat gaagcggata aggtttcaat atttggagga atagtaggaa taacttctac
    44641 tatagataaa gctacagcag agaaattaaa tgaaatattc ttagaaatag tagtagcata
    44701 tgattttgaa ccagaagctt tagagatact taaacaaaag aaaaatctaa gaatattaaa
    44761 attagctaaa atagaaaata gtttacaacc atatgaaatg aaatatttag atggaaaatt
    44821 attgatacaa gatagaaaca atatattagc agaaaaaagt gaaaatgtaa caaaggaaaa
    44881 accaacagat gcacaactta aggacatgga atttggtatg agagtagtaa aaaatatgaa
    44941 atcaaatgca attgcagtag taaaaaatgg acaaactcta gctttaggtt gtggacaaac
    45001 atcaagaatt tgggcattaa aaaatgctct tgaaaataac aaagataaag attttacagg
    45061 agctgttttg gcgtcagacg catttttccc atttgatgat tgtgtaacat tagctcatga
    45121 atatggaata tcagcagtag ttcaaccagg tggctctata aaagataaag attcaataga
    45181 agcttgtgac aagtatgata tggttatggt atttacagga ataagacact tcaaacacta
    45241 aggaggatat tatgaagata ttggttgtag gcggtggcgg aagagagcat gctatatgct
    45301 ggaaactaag caaagaaaaa aatgtagaaa aaatttattg tgcaccaggt aatgcaggaa
    45361 ttgcaaacgt agcagaatgt gtaaatatag gtgatactaa tatagaagaa cttttaaaat
    45421 ttgccaaaga aaatgaaata ggtctgacaa tagtaggacc agaagtacca ctagttatgg
    45481 gaatagtaga tgagtttgaa aaagaaggat taagagtatt tggacctaat aaaaaatgtg
    45541 ctcaacttga aggaagtaaa gctttttcaa aagaatttat gataaaacat aacataccta
    45601 ctgcaaaata taaagaatat acaaacttag aagaagctat atcagaaata gatagttttg
    45661 gatatccagt agtaataaaa gcggacggat tagctgctgg aaaaggtgtt gtaatacctg
    45721 aaaatagaga agatgctata gcaactttaa aagaaatgat gtcagataag aagtttggag
    45781 ctgctggaga taaaatagta attgaagagt ttttaaaagg aatagaaact tcaattctag
    45841 catttgtaga caatgataca atagttccta tggcaagtgc aaaagaccat aaaaaagtaa
    45901 acaactatga gcaaggtcca aacactggag gaatgggaac tttttcacct agtgaaatat
    45961 atacagaaga attagcaaat aaagtaaaag agacagtatt agaaaaaact ttagaaggtt
    46021 tcaaaaaaga tggacttaat tttaaaggta ttttatttgt tggtcttatg ataactgaag
    46081 atggagaaaa agtacttgaa tataatgtaa gatttggaga ccctgagaca caatctgttt
    46141 tatttagatt agaaacagat ttacatgaga taatggaagc tatactagat aataagttaa
    46201 aagatataga aataaactat agtgatgatg aagctgtatg tgttatgctt acatcaggag
    46261 ggtatccaga cagttatgaa aaagggaaaa ttataactgg tcttgaaaac ttggatgatg
    46321 atatagtagt attccatagt ggcactaaga tgtttgatgg taatttggtt accaatggag
    46381 gaagagtaat tggtataact gcaaagtcaa ctagagttaa ggatgcagca gaaaaagttt
    46441 atgaaaacat aaagaagata aactttgaag gaatgcacta tagaacagac ataggaagat
    46501 aatttttcta aaatggagga taaggtatgt tgaatactga aaataaagac tcaatggtta
    46561 gaagagtatt ggttgaaaaa agagaaggtt ttgaccttga ggctaaagct ttaaaaaaag
    46621 acttagtaga aagtttacac atagataata tagaaaattt aagaatatta aatagatatg
    46681 atgtagaagg aatatcagaa gaagtttatg aaaatgcagc aaagacaata ttctctgaac
    46741 caaatttaga tgtagtatat tatgaagaaa taccaaaatt aaatgatgaa agagtatttg
    46801 caatagaatt tcttcctggt caatacgacc aaagaggtga ttgggcagct caatgtgtac
    46861 aaattgtaaa tcaagggata agacctgcta ttaatacagc aaaagtttat atattatcag
    46921 gcaaaataac tgatgaagag ttttctaaaa taaaagatta ctgtataaat ccagtagata
    46981 gtagagaagc atctttagag aaaccagaaa cgttaaaaat ggaaactgaa ataccaacaa
    47041 cagttgaagt attagatgga tttatagatt tagatgaaaa tggattaaga acatttgtaa
    47101 gtgaaaaagg attagcaatg actttaggag acttacaaca cgttcaaaaa tactttaaag
    47161 atacagaaaa gagaaatcca actataacag aaataaaagt attagatact tattggtctg
    47221 atcattgtag acatactact tttatgactg aaatagaaaa tgtaaagata gaagatggta
    47281 agtttaatga tatagtaaaa gaagcgtatc aaatgtatct taactcaaga gacaatgtat
    47341 atgtgaacag acataaagat atatgtttaa tggatatagc cactgttgca gtaaaagaat
    47401 taaagaaaaa tgggaaatta aatgacctag atgaaagtga agaaataaat gcatgtagta
    47461 taaacgtaga tgttgaagtt gatggtaaga tggaaaaata tctagtaatg tttaaaaatg
    47521 aaactcacaa ccatccaact gaaatagaac catttggtgg agcagctact tgtttaggag
    47581 gagctataag agacccatta tcaggaagaa gttatgttta tcaagcaatg cgtgttacag
    47641 gaagtgctga cccaagaacg actttagaag atactttacc tggaaaactt atgcaaagaa
    47701 aaataactac agaagctgct catggatata gttcatatgg taaccaaata ggtcttacaa
    47761 ctggacaagt tgcagaagta tatgatgaaa actttgttgc aaagagaatg gaaataggtg
    47821 cagttatagc agcagcacca aaagagaatg tagtaagaga aagacctgaa gcaggagatg
    47881 taatcgttct tcttggagga aaaacaggaa gagatggctg tggaggagca actggttcat
    47941 ctaaagaaca tagtgaagag tcaatcttaa cttgtagtgc agaagttcaa aaaggagatg
    48001 ctcctaacga aagaaaaata caaagattct ttagaaataa agaagttgct caaatgataa
    48061 aaagatgtaa tgattttgga gcaggtggag tttgtgttgc tataggagag attgctgata
    48121 gcttagatat aaacttagac ttagttccta aaaaatatga tggacttgat ggaactgagc
    48181 ttgcaatttc agagtcacaa gagcgtatgg cagtagccat aaaaaaagaa aataaagata
    48241 aatttataca attagcagta gaagaaaact tagaagctac gcatgtagct acagtaacag
    48301 atacaggata tttaagaatg ttctggaatg gaaaagcaat tgtagatata aatagagagt
    48361 ttttagatac aaacggagta aaacaaacta ctgatgttca tgttactaaa gtagatgaag
    48421 aaaatacatt ctttagctct aatgaaatag taaaagatgt taagtgtgca agtatgaaag
    48481 acaagtttac aaaagtacta tctgacctta atgtttgttc tcaaaaagga ttagtagaga
    48541 tgtttgataa tacaattggt ggtaatacag tacttatgcc atttggagga aagtatcaag
    48601 cgactccaac acaaggtatg gttgctaaga taccagtact tggaggagaa actaatactt
    48661 ctacaataat gacttatgga tacaatccaa aggtaggaaa atggagccca ttccatggtg
    48721 ccttgtatgc agtagtagaa tctgtgtgta aattggttgc tattggagga aattatagta
    48781 caacaagact tacattccaa gagtattttg aaaaacttgg taacaatcca gaaaaatggg
    48841 gtaaaccatt ctcagcactt ttaggagcgt tttatgcaca aagtaaattt gagatacctg
    48901 ctataggtgg aaaagacagt atgtctggta catttaaaga tattgaagtt ccaccaacat
    48961 tagtttcatt tgctgttgac acagtagatg cgaagaaagt agtatctcca gaatttaaaa
    49021 aagcagactc aaaagtagta atgttatgtg taaacaaggc agaaaatgat gttgtagatt
    49081 ttgaggaatt aaagagaaac ttagataaag taagagaact tatacatgga aataaagttt
    49141 tatcaacata tgcattagga tttgcaggtg taggtgaagc aataagtaaa atggcttttg
    49201 gtaataaaat aggatttaag tttagtgaag aagctgaaaa agcatttaca gataataaat
    49261 tatttgaggc tagttatgga aatatagtac ttgaattagc aaatgatgat ttaagtatgt
    49321 tagaaggata caattatgtt gtgattggaa gtacagttaa agaagctagt atatttatca
    49381 aaggtgaaga acttgcatta gatgaattat ataaagcaca ttgtagtact ttagagccta
    49441 tattcccaac taaaacagaa gaagtaaaat caaagataga aactataagt tatatatcac
    49501 aaggagaagc taaaaaatca tcattaagta tagctactcc aagagtattt ataccagcat
    49561 tccctggaac aaactgtgaa tatgactctg caagagcatt tgaaagagca ggagctaatg
    49621 caagtataag agtatttaag aatttaactt ataaagacat agaagattct atagatacaa
    49681 tagtaaacga aataaaatct tctcaaataa taatgttacc aggtggattt agtgctggtg
    49741 atgagccaga tggttctggt aaatttatag ctactgtatt tagaaatcct agagtgcaag
    49801 aagctataaa tgaattttta actcaaaaag atggactaat gttaggtatc tgtaatggat
    49861 tccaagtatt aattaaatta gggttagttc catatggaga aataagagtt ccaagtgaaa
    49921 gtgctccaac acttacttac aataatattg gtcgccatca agctaagatt gctagaacta
    49981 gaatttcatc taataaatct ccttggttag ctcaaactaa tgttggagat attcataata
    50041 tagcaatttc tcatggagaa ggtaagtttg ttgccagtga agatgttatg agagaattaa
    50101 ttgctaatgg acaagtagca actcaatatg ttgactttaa taatgaagct acttatgata
    50161 ttgagtttaa tccaaatggt tcattctatg ctgttgaagg tataactagt gctgatggta
    50221 gagtgtttgg taaaatggga cattctgaaa gaattggtga ggaagtatat aagaatatta
    50281 taggtgaaaa agaccaaaag atatttgaat ctggagttaa atattttaga taaagctttg
    50341 atgtttaatt gatagctaga atatataatt tagatattta gatttaaaga gatgtaataa
    50401 tatgatgata atcatttaat attacatctc ttttttattt atctaagaaa taataagttc
    50461 aacctaagtt aaaatacaag ctgtgttata attaattatt aagtaaaatt gaatatttat
    50521 tggagggcaa cataattaat gaaaatatta ataacagggt ctaatggtca attaggaaaa
    50581 gaattggtta accaattaga agcaatcaat caaagtataa accaaccaaa atatgttata
    50641 ttagctacaa caagaagtga tttggacata tccaatcaga ctaatgtaga taattttatt
    50701 ttgcataata aacctgatgt agttgtaaac tgtgcagcat atacaaaggt tgatgcatgt
    50761 gaagataata tcgaaattgc atacaaaata aatgctttag gagtgagaaa tctagcaata
    50821 gcatcagaaa aagttaatgc taagcttatt cacatatcaa ctgactatgt attcaatggt
    50881 ttttctaaat atccatatag ggaagacaat aaaactgaac ccaattctgt atatggaaaa
    50941 agtaaactta tgggagaaaa atttgttgaa caattctcac ataagtactt tatattgaga
    51001 acagcatggt tatatggtaa tgggaataat tttgtaaaga ccatgataaa actttcttta
    51061 gaaaataaag aggttaatgt ggttaatgac cagtttggaa ctccaacaag tacagtagac
    51121 ttagcaaaaa ttataataag aatcatggaa acagaacatt atggagttta tcatgctaca
    51181 tgtgaaggtg agtgtagttg gtatgatttt gccaaaaaga tatttaaact aaaaaatata
    51241 gatataaaag taaatccaat taagagtaac gaatttaaat gtaaggctcc aagaccacta
    51301 tattctgtat tggataattt tatgttgaag ttaattggtt taaattcttt tagaaagtgg
    51361 gaagaatcca ttgaagaata tttagaaaga tatgatataa atgaaaagta aatgtattat
    51421 tttgagaaaa gaaaataaga tattataaaa aatataaata tttcttttat ttatccgata
    51481 atcataatat gacaaaaaca aatttaagga tggaaaaaga tgaaaggaat tattttagca
    51541 ggagggtctg gtacaagact atatcctata acaaaatgta tatcaaaaca aacacttcca
    51601 atatacgata aaccaatgat atattaccct atgtcggttc ttatgttggc agggatacaa
    51661 gatatattaa taatatctac accaagagat ataaaagcat ttgaagaatt atttggagat
    51721 ggaagttcac tagggcttaa cataacatat gcaattcagg agaaagcaaa tggaattgca
    51781 gaagcgttta taataggaga agaatttata ggaaatgaca gagtgtctct tgtattaggg
    51841 gataatatat tttatggata tggatttagt gaaaggttgg agaatgcagt taaaagaaaa
    51901 ggagcaacta tatttgggta tcatgtaagt aatccaaatt cgtttggagt tgtagaattt
    51961 gataaggatt ttaatgtatt gtcaatagaa gaaaaaccta cagttcctaa atctaattat
    52021 gctataccag gattatattt ttatgataat gatgttgtag agatagcaaa gctagttgaa
    52081 ccgtcagata gaggtgaatt agaaataacc tctataaaca gtgaatatct aaaacgagga
    52141 aaactaaaag tggaacttct aggaagaggt atggcttggc ttgatactgg tacacataaa
    52201 ggtctattgg atgcttctaa ttatgttgaa gtggttcaaa caagacaggg actgtatata
    52261 gcatgtttag aagagattgc atatacaaaa ggatatataa gtagagaaca attaatgaaa
    52321 ctagccaagc cactgataaa aactgaatat ggtcaatatc tattaaaatt ggctagtgaa
    52381 gatatgtaat atggtagata aaatttatta taaattttat taagggagtt tattataatg
    52441 ggaaaaatca atgttataaa gactaaaata gaaggattat atatagtcga accaaatgta
    52501 ttttatgatg aaagaggata ttttatggaa agctataata aaaaggattt tatagagcag
    52561 ggtcttgata tgtgttttat tcaagataat gagtctagct caagaaaagg cgttttaaga
    52621 ggaatgcatt ttcaggttaa atatcctcaa ggaaaattag tcagagttac agagggagaa
    52681 gttttcgatg ttgcagttga tttaaggaaa gattcacaaa catttgggaa atgggaaggt
    52741 gtaatattgt ctgataagaa taaaaaacaa ttttatattc cagaagggtt tgcacatgga
    52801 tttttagttt tatcagaaaa agcagtattt agttacaaat gtacaaatta ttattaccca
    52861 gaatttgaga atggctttat atggaatgac aagcaaatat ctattgagtg gcctttaaaa
    52921 ggaattgaag aaatattttt atcagaaaag gacaaaaaac aaaaaagttt taaagatatt
    52981 tgtacaagac gttaataaaa atttattaga attaacattt aggagaaaaa atatgaaaaa
    53041 aatactgata actggaggag caggatttat tgggagtaat tttatacatt atatgcttaa
    53101 caagtatgta gattatttta ttgttaacct agacttgttg acttatgctg gtaatttaga
    53161 gacattaata gaaatacaag acaattccaa ttataaattt gttaaaggag atatttcaaa
    53221 tagggagcag gtgtataagc tatttgaaga agaaaggttt gatatagttg ttaactttgc
    53281 cgctgaatct catgtagata ggtcagttga gaatcctgat ttatttatta aaacaaacat
    53341 tataggaaca caagtacttt tagatgcatc tataaaatat ggagttaaaa ggtatcatca
    53401 aatatctaca gacgaagtgt atggtgattt accaattgat agaaaggatt tattctttac
    53461 agaacaatct tctatcaatc caagttctcc atattcagct tctaaagcat cagcagattt
    53521 attggtaagt tcttattata gaacatatgg attgttaaca actatatcta ggtgttccaa
    53581 caattatggg ccatatcatt ttcctgaaaa attgattcct cttatgattt taaatgcttt
    53641 ggaaaacaaa caattgccag tatatgggaa tggggaaaat gtaagagatt ggttacatgt
    53701 atatgaccat tgtactgcta tagacttaat aatacataaa ggaaatatag gggagattta
    53761 caatatagga ggtcataatg aaagaagtaa tctagaagta gtaaaaatga tattaaatct
    53821 tttaggtaaa tctgaagagt taatatccta tgtaaatgat agacctggtc atgatttaag
    53881 atatgctatt gatgcaacta aaatagagaa tgagttaggt tggaaggcaa aatatgactt
    53941 tgatttgggt ataaaagaaa ctgttaagtg gtatatagaa aatgaatctt ggtggaaagc
    54001 tgttttatca gtataacttt aaaatttaga aaggggcgtt ttatattgaa tgagataagt
    54061 tcactaatta atatattagc attgactcaa ttatctggta gtaatactac aaatcaatgt
    54121 aattgtacat ataataattc atctggattt gatatgatta tgatgacctt gttaaaagca
    54181 ttatcacaaa ataatcaaca tacttctaat agctacaatt tattgtctaa ttctgagaat
    54241 ttatttaatg aacttgataa tgtagtaaat aataccacta gtagatttat agatgttgat
    54301 acaaaagata aaaatgtaaa gttgagaatt gaaaatgctg tagatcaagc atcaaaaaaa
    54361 tataacgtag attcaaatct tataaaagca ataattaaag ttgaatcaga ttttaatcca
    54421 aataccgttt cttctgctgg tgctaagggt cttatgcaac ttatgccaga aaactgcaga
    54481 gacttaggag ttacagaccc ttttaatata gaacagaata tagatgctgg aactagacat
    54541 attaaggaat acattgatat gtttggaggg agcatagaaa tgggattgat ggcatataat
    54601 ggtggtcctg gaagaatgaa aagtagagga gtagaatcaa tctctgacct gtataaaatg
    54661 ccaaaagaaa cacaaaatta tattcctaag gttatgaaat attatagagg gtaggcttta
    54721 ttatgggaat aaatatagat aagctaaaca taagtccaag tataaatatg gaggttaaaa
    54781 aactaaaaga gagtaataaa ttttctgaca aattaaactc tttaaaacaa tcttcttcat
    54841 tggaagatat aaaacgaaat ttggaaagtg taaaaaaggc aggagaaagg cttgtactta
    54901 ctcaaaacta tggcgatatt agtatatata aaaatgcagt aaaagaatac ctaaaatctg
    54961 ttgtagataa tatgtattca cttgataaaa atagtagctt ttgggagcat cagtattata
    55021 agaatgtaaa gattgttgat agtaaacttg aggatatgac tagtaagtta attagtgaag
    55081 aaaaagaaaa catagatatt gtttctactg ttgacatgat acaaggctta ttgatagata
    55141 tgtatagata aggttataca aggagaaaaa ttttatgaat agcaaaaaag aggatatata
    55201 tgaattgaat tttgggcaat taatagaaga aaaatctggt gttatagaag aaaatcaaaa
    55261 tgtttttaat gcaaaagtaa atgtagctgt ttctatagga aatacaacag aaacaataaa
    55321 aaatatatta gatttaaaag aaggctctat aataaaatta agtaagaatg tggatgaaaa
    55381 attagatata tatgcaaacg atagattgtt tgcttatgga gaaagcatta ttacaaatga
    55441 gaggttatct gtaagattat ctaaaataga agatccagat gtaaattagc attttaaata
    55501 gtatcccttt aggctattct gtatgagttt agctaagggg atatttttat aaaaaattta
    55561 aaatatgtgt gaattatata aatattttta ttttatgagc gataactaaa ttataagtat
    55621 gtaattttgg aggtgaaact atggatatta aaaatgtaag ttcaaatatt gtagtgaata
    55681 attatttaaa gaatacatct ggtataaaaa aagtggaaga ggttaaaaaa attaataaaa
    55741 cagataagat agagttatct aaatttgcaa aggaaatggc aagtgtgtca aatatgagtg
    55801 atgagaatat tagaaataaa aaagtcgatg aaataaaaaa tgcattagaa aatggaactt
    55861 acaaaataaa accaaaagaa attgcgaaaa aaatgataga ggatatgcga ggataaagtc
    55921 atgattacag acttaaaagt tataatatat gaagaaaaaa atataattag taagatgatg
    55981 gaattattaa aagaacaatt tgattatata gttgaaaaag acttagaaaa tttaaatatg
    56041 ttaaatccta aactagaaga tattagcagg gagcttgcat ccatagaaat aaagagaaga
    56101 caactttttg gtgatgatat ttcaatgagt gaagttgttg aaaatagtaa tgatggatac
    56161 ttaaaagaaa tatatacaga cttgaaacac attcttgctt tagctaaaaa tcaacaagaa
    56221 tcaaatgatt ctctaataaa gaaagaactt atttttacga aaaaaatgat taatttcata
    56281 aaaccagctg ataagcaaac tacaacttat aattcttatg gaaatattag aaaataaata
    56341 ttaggaggag aaatatggca ggattatttt ccattttaaa tacagctaat tcaggaatga
    56401 atgcgcacca aaaagcaata cagacaattt ctcataatat aagtaatcta gatacagacg
    56461 gttattctag gcaaaggacc gagtttgcca caaatagtcc aatgtatatg cctagtttga
    56521 gtaattcaat tgcaagagga caattaggga ctggagtaca tgtaacggat gtaacaagag
    56581 ctagaaatag tttttatgat taccaattta gggcagaagc tcataagtat ggtaagacag
    56641 tttcaaaata tgattattac aatactatag aaactatact aaatgaacca tcagactatg
    56701 gaatatctgc aggtatagat gattttttta aaggatggga gtcattatca aaggatataa
    56761 atagtgacag taaaaaaagt ctagtggtag aaaacgcatc taatttagca actttaataa
    56821 gtgaaagtta taagaagctt gataagttaa aagaagatat agattctaat atagacactg
    56881 agataaaaca aataaatgac atgttaaaaa gtcttgaaga tttaaataaa tctattgata
    56941 ttatatctgg ttctggctct acaccaaatg atttacttga tgaaagagat agaattttag
    57001 ataacttgag ttttaaacta gactttgaaa atagtgatgt taaaaatatg cttgcagatg
    57061 gtaaattaga acttaatgaa ttaaaaaatc cagatggaac ttggaaaaca ggtatatcag
    57121 ggaccttaca aggcttgttt gaaatgcatg gtaagattga tacatataaa agcgatttaa
    57181 aagatgtatc tgatggatta gctaaacaga taaatgatgt ttataatacg tctgcaggaa
    57241 taacagtaac agattttttt ataacttcaa atgtagatgg tgaagatatt ataaagataa
    57301 atccagctat aaagagtaac ccaaatgaat taaagcttac tgctgatgaa gcttcaaaaa
    57361 ttgctaagtt aaaggatgaa aaaatagata ttggggtggc tggtggaaaa gtaagtacaa
    57421 taagtgacca ctataaagcc tttgcagaat caatcggttt agattctcaa aaagtaaatc
    57481 aagatgaagc aaatcaacgt aaaattataa ataatattga taactcaaga atgagtgttt
    57541 caggtgtatc tttggatgaa gaaatgacag agcttataaa aatacaacgt tcatatcaag
    57601 ctagtgcaaa agttatgtca acagctgtgc aactactgga tgttgtaata aatggaataa
    57661 tttaatagaa gggggaattt ataatgagag tcagtacagg tatgatgtct agcagctatc
    57721 taaactcact acaagataat ctacaaagat tggataaggt taatagacaa gttaatacaa
    57781 caaaagaaat aaacaagcta tcagataatc catataaagc aataaagata ttaaactcaa
    57841 aaagtgaaat aaagactatg gagacttatg ttgagaattg taaagataca gggaattggt
    57901 tagaaactac tgatactgca ttagatcagc ttggaaaatt attatcagac ataaagaaaa
    57961 ctttggtggc aagtggtgat ggtgcatact cagatgatga attaaagact cttagtaatg
    58021 aaactaatga aaaaatgaaa gaaattgcaa atgctttaaa tgcaacacat gaagggaagt
    58081 acatattttc aggttcaaat acgggggtac cacctgttga atgtatagat aatgctgatg
    58141 gttcagtgag tttaaagttt aattctagtc taaatcttaa taaattaaat gactctttga
    58201 gtatggatat agctcaagga ataagtgtag attataacgt taaattgagt agccttggat
    58261 ttgacccaac aaaaactgac ccatttgaag agttaaataa catatcaaaa aaattagcaa
    58321 atccagatgc tgatgctata aaagagctta caacaacttg tttgggcgat atggaacaac
    58381 taatagaaaa tacagtaaat gttaggtcta tatatgggac aaaagcaaat acagtagagg
    58441 ctatgaagga aaaaaacgat gatagtttga tacagttaaa ggatattttg tcacaaaatg
    58501 aagaaataga ttatggtgaa aaacttgttc aattaaaggc agcagagtta acttatcaag
    58561 cttcactaca aacaggagga aagttattta atgtatctat attagattac atataataaa
    58621 ataacgcaga tatgatgaag gttacattaa aaaaaggtat attaggtttt gaaaatttaa
    58681 aagaatatga gcttttagat atagaagata atgattattt aaaagaattt aattctactg
    58741 aagaagaagg tataggattt gttgttgtat ctccatttga gattatcaat gaatatgaaa
    58801 tagttttaaa tcaggaaaca atagaaaaac tagaagtgaa atctccaaag gatgttgtat
    58861 tatttaatat aattactctt ggtcagacat ttgaagaatc aactgtgaac atgaaagctc
    58921 ctatagtgat aaatataaaa actaattttg gaatgcaaat aattttacag gatgataaat
    58981 attcaataag gcatccatta ttgagaggtg atagtggatg ctagtaattt caagaaaaaa
    59041 agatgaagca gtacttatag gagaaagcat agaagtaaaa attgtagggg tagatggaaa
    59101 caatgtgaaa ctagctatat cagcgcctaa caatataagt atattaagaa aagaaatata
    59161 cgaaaaagta aagagtgaga atataaaagc tacaaataaa aatataaaaa ttttaaagtc
    59221 attaaaataa taagcttgga ggaagtaata tatgtatgga gaaaatcctt ataattctta
    59281 taaacaaaat gctgttttta tggcatcaaa ggaacaattg ttacttatgc ttgttgatgg
    59341 agcagtaaaa tataccaaaa tagccaaaac agctatctta gataaaaata cacaaaaagc
    59401 gcatagagag cttataagag ttcaggatat atttacagaa ctaatggtta cattagacca
    59461 aaatgcaggt caatgggcaa aggatatgta cagagtttat gattttgtaa gatatgaact
    59521 ttcaagagct aatattagaa aagacattca aataatagat aatgtgttgc ctgtaataga
    59581 acaaattaaa gatacttggc atgaagctga taaaaaaagt aaagaagaaa gaagtaggta
    59641 caaataatgg aattaaattt agctgaatta gataagttat caaaagagga attactattg
    59701 atgcttgtag atggaacagt aaaatatacc aatatatcca aagaagctct tttaaataac
    59761 gactatttga aagcacataa tgaacttgta agagttcaaa atatatttac agaactaatg
    59821 actacattag accaagatgc aggtcaatgg gcaaaggata tgtataaagt ttatgaattt
    59881 ataaaatctg aattagcaat agctgatgaa aataaagata ttaaaataat tgatgatata
    59941 ttgccaatag taaaacaaat tagagataca tggcatgaag tttacaatca actaaaaata
    60001 taattggatg gagggataaa ggtatgtcaa gtataagtcc tataagagtt acaggtcttt
    60061 caggaaattt tgatatggaa ggcataatcg aagctagtat gattagagac aaggaaaaag
    60121 ttgataaagc aaaacaagaa caacaaatcg ttaaatggaa gcaagaaata tatagaaatg
    60181 ttatacaaga atcaaaagat ctttatgata aatatctaag cgtaaattct cctaatagta
    60241 tagtaagtga aaaagcatac tcttctacaa gaataaccag ttctgatgaa agtattatag
    60301 tagcaaaagg ctcagctggt gcagaaaaaa taaattatca atttgcagtt tctcaaatgg
    60361 ctgaaccagc aaaatttact attaaattaa attcaagtga acctattgtt caacagtttc
    60421 ctccaaatgc tagtggagcg agttctttaa ctataggaga tgtaaatata ccaatatctg
    60481 aacaagatac tacaagtact attgtaagta agataaactc cctttgcgca gataatgata
    60541 taaaggcttc ttatagtgag atgacaggtg aattgattat ttcaagaaaa cagactggtt
    60601 cgtcatcaga cattaattta agagtaattg gaaatgacaa tttagctcag caaattgcta
    60661 atgataatgg tatcacattt ataaatgatg ctggtggaaa caaagtggca aatgtatatg
    60721 gaaagaatct agaagctgat gtaactgatg aacatggaag agtaactcat ataagtaaag
    60781 aacaaaattc atttaatata gataatattg actataatgt aaattcaaaa ggaactgcaa
    60841 agttgacttc tgtcactgat actgaagaag ctgttaaaaa tatgcaagca tttgtggatg
    60901 attataataa actgatggac aaggtctatg gtttagttac tactaaaaaa ccaaaagatt
    60961 atccgcctct tacagatgcc caaaaagaag atatgacaac tgaagaaata gaaaaatggg
    61021 aaaagaaagc taaagaaggt atacttagaa atgatgatga gttaagaggt tttgttgaag
    61081 atattcaatc tgcatttttt ggagatggaa aaaatattat tgcattaaga aaactaggta
    61141 tcaatgaaag tgaaaattac aataaaaaag gtcaaatatc atttaatgca gatacttttt
    61201 caaaggctct tatagatgat agtgataagg tatacaaaac actagcaggt tattcttcga
    61261 attatgatga taagggaatg tttgaaaagc taaaagatat tgtatatgaa tattctggaa
    61321 gttcaacttc taaacttcct aaaaaagcag gtatagaaaa aactgcttct gctagtgaaa
    61381 atgtatattc aaaacaaatt gcagagcaag aaagaaatat aagcaggtta gttgaaaaaa
    61441 tgaatgataa agagaaaaga ctttatgcta aatattcagc cttagaatct ttgttgaatc
    61501 agtattcttc ccaaatgaat tatttctcac aagcacaggg taattaatta tggataaatt
    61561 aaaagaaaaa ttaaatttat ataaagatat atctttacaa attataaatt tgattgaaaa
    61621 ggaagaatat ataaagataa gtattaagtt aggtgaaaga caagagatta taaatagtgt
    61681 aagtgaaatt gatagaaatg attttataca gctttataat agaatggaat taattgaaat
    61741 tgatagcagg ataagagata ttttacaagg acaattatta gaagtaaaaa aagaattaca
    61801 tgagtataag cttactaaac aagtgaacac aatgtattat aacttaaata gagaaaaagt
    61861 aaatattttt aataaaaaag tataaagtta tgtaccatct tgtccgatta tataaatgaa
    61921 gaattaataa aaaagattaa aagtagaaat gacaaggatg tcaactatac taaggagggt
    61981 aaaataatga gagttaatac aaatgtaagt gctttgatag caaataacca aatgggaaga
    62041 aacgtaaatg cacaaagtaa gtctatggag aagctatctt ctggtgttag aattaagaga
    62101 gctgctgacg atgctgctgg gcttgctata tctgagaaaa tgagagctca aataaaaggt
    62161 ttagaccaag caggaagaaa cgttcaagat ggtatatctg ttgtacaaac agcagaaggt
    62221 gcattagaag aaactggtaa catactacaa agaatgagaa ctttatcagt acaatcttca
    62281 aatgaaacta atactgctga agaaagacaa aagatagctg atgagttact acaattaaaa
    62341 gatgaagttg aaagaatatc aagctcaatt gagtttaatg gtaagaaatt acttgatgga
    62401 agttctacag aaataagatt acaagttgga gcaaactttg gaacaaatgt tgcaggaaca
    62461 tctaacaaca acaatgaaat aaaagttgct ctagtaaata cttcaagtat aatgagtaaa
    62521 gcaggtataa caagttctac aatagcaagc ttaaatgcag atggaacttc tggaacaaat
    62581 gcagctaaac aaatggtatc tagcttagat gtagctctta aagaattaaa tacatcaaga
    62641 gcaaaattag gggcacaaca aaatagacta gaatcaactc aaaacaactt aaataatact
    62701 atagaaaatg ttacagcagc tgaatcaaga ataagagata cagatgttgc ttcagaaatg
    62761 gttaacttat ctaaaatgaa tatattagtt caagcatcac agtcaatgct tgctcaagct
    62821 aatcaacaac cacaaggagt tttacaatta ttaggataaa ataattttgt atattggata
    62881 tagaagaaag ggtaaggctt tgctttactc ctttcttttt ttacaaatat acttttgaaa
    62941 ggaaaattta tatgttactt agtatagtta tgattgttaa aaatgaagaa aaaatacttg
    63001 aaaaaacttt aaaatcatta accacattaa gaaattctat agaaagtgaa cttataatag
    63061 tcgatacagg ttctacagat gatactataa aaatatctaa gaagtacacg gaaaaagtgt
    63121 attttcataa ttggaataat gatttttcta gtatgagaaa tatatcaata tcctatgcta
    63181 aaggtgaatg gttacttata ttagatgcag atgaaatact aattgattct tcaactattg
    63241 taaaattttt taaagatggt cttgataaaa aatttaattc tgcttcaata caattaaaaa
    63301 atctttactc atatgataaa aaattatatg gatattgttc agtgcttaga ctttttaaaa
    63361 acgttgattt tagataccat ggtaaagtac atgaacaacc aatatacaaa aaccctatat
    63421 ttaataatgt tgctgatttt gaacattatg gatatttgtt tgaagatgaa gaaataagat
    63481 taaataaggt gaaaagaaat gaaaaactat tatttcaaga gttaaaagag aatgataatt
    63541 ctccatatac aaactatcaa ttagggaaaa attttattat attaggaaaa tatcaggagg
    63601 ctttatacta tcttgagaaa tcttatgaat tatattctaa actagaattg gcacctggtt
    63661 acttgataac aagccttgta aaaacatatt tatatttagg aaaacataag aagtgtgaaa
    63721 gattgtgctt aaagtatatt aagaaagaca tgaataatat tgatatatat tattatattg
    63781 cacaagcaca agtagattta ggaaagtatg aaaatagtat agattcgtat aagagatata
    63841 tttacttgtt agataattat gaaataagta cacaagcaaa tagtttattt tcagacactg
    63901 atactgtagg gcttagagat gaggctatta ttacactgat aaagatttat tataagcttg
    63961 aaaaatatga gtttgtaatt tcagagtata ataacattga agatgtggaa aagaaaaaga
    64021 atgtgtactt tagcttattt atgtcactat ataaattgaa tagatttgaa gatataaaaa
    64081 attattataa agaagtttca aattctaaaa ttgaaaaaaa tagtttttat agaaatatag
    64141 aagaagttat aaataatatt aaagaggatg aaaaagagta tgtatataaa atcctttgtg
    64201 acattgaagg taattatggc attttaaatc aaataaggat aaataaatgt gtatctatag
    64261 ataaatgtaa agaaatactc aatgaagaaa aggaaataat ttatgcatat attataaaaa
    64321 tagcttttga agagaagatt gatttactag atatttttta tgaaatggaa tatgtatgga
    64381 tagagaaata tctaaaatat cttttagcat tagaaagaag ttttaattta gtactatata
    64441 agtacataat tagtcgtcca aatacatcag atatggaaaa gataagagta tataaagcta
    64501 tatctaaagt agttttagaa agtataagct taagtgatga aaaatataag gagatttttt
    64561 atctttatat aatgtattct tataagtaca taagatacat atatagcaat ttaagtgatt
    64621 ttgaattaat aaaatttgtt tgtaatgatt tagataagtt cactatagaa tttaagcatc
    64681 tacttgatat taaaaataaa gtaaataatg ctaatgaaaa attagagtat atacatagta
    64741 tgaggaaatt actaaatgag tatccttttt ataataaaat aataaaaata cttataggtg
    64801 aattagaaga aaatataaaa gaaagtgaag aatttaagga tttaaaagat agttttatta
    64861 ttagtataga aaatatgatt gaaactggca aaataaatga tgcgaaatta ttggtagatg
    64921 actattcaaa aacatttgtg gatgatataa agattttaaa tataaaggga attttataca
    64981 tgtttgaaaa taagcttgat aaagctgatt ttatgttaaa aaaggcatta tcattagatg
    65041 tagaaaatga agatactata tataatatag aatacttaaa aagtttaaga taagtcaaaa
    65101 ataggagaat tgaagaatga atataccaat agtggttaat gaaaaatcta aagacataat
    65161 aaaagattgg caatatacat gtacaaatga agatataaaa tttacaagca taataatatt
    65221 gacatataat caaatagaat atactaaatt atgtattgaa agtattagga aatttacccc
    65281 aaagaataaa tatgaaataa taataattga taataactct tctgatggaa cagttgagtg
    65341 gttaaaggga caatctgata taaaatctgt gtataataac aaaaacttag gatttccaaa
    65401 aggatgcaat caaggtattg agttagctac tggtgaaaac atattgttgt taaataatga
    65461 tgtaatagtt actccaaatt ggctctataa tttgaataaa gctttatggt ctcaagataa
    65521 tataggtgct gtaggaccta ttacgaatgc atgttcatat tatcagcaaa ttgatgttga
    65581 ttataagtca attgaggaca tgtttatatt tgcacacaaa ataaataaat ctaatattaa
    65641 cctatggaaa tatagattga agttagttgg attttgcatg ttaataaaaa aaactgttct
    65701 ggataatgta ggggttttag atgaattatt cacacctgga aattttgaag atgatgattt
    65761 atcttttagg atgatagagt caggatataa gttattatta tgccaggata cttttataca
    65821 ccattttgga agtgtgtctt ttaaagaaaa tcgtaataaa tatacagata taatgtatat
    65881 taatagaaga aaatttgaaa ctaagtgggg atttaatcca gaatatagtg gaatgataag
    65941 aacagatttg atttctaaaa tagatgatag tttggaggca aatattaatg ttttagaaat
    66001 aggatgcggt ataggggcta ctcttttaga aataaaaaat atatataaaa atgcatcaat
    66061 atatggaata gaaattagtg agtcagcagg taaacttgca aagtatagtg cagatgtact
    66121 tatagcaaat atagaaaaag taaaattgga ttataaaaaa gatttttttg attatataat
    66181 attgggagat gtacttgaac atttgattga cccatggaag atagtcaatg aattgaaaca
    66241 atatctaaag aaggatggtt atctggtatc tagtatacca aatattatgc atgtttctgt
    66301 tttaagaaat cttgtgttag gtaattttac atatgaagat tcaggaattt tggacaagac
    66361 tcatttaagg ttttttacat taaatgagat aacaaaaatg tttaatgata gtaattacat
    66421 aattaatagt attacagcta ctagtatgcc actttcggaa attgataata gcttcattga
    66481 tacaatatgt aaggaaagct gtaaagaaat gagacaacag tacgaatctt atcaatatat
    66541 agtaaaagcc tctaaaatcc tagatactaa aagatattct agtattagta tgattaattt
    66601 aaaatacgct ttaatgagaa ttgataatga attagatgta gatagtaatt taagttatat
    66661 atttgataat tataatataa ctgaaccatg ttttgttgat gatattgaat atctaattga
    66721 tacatttgtg ataaataaag caactctatt aaatagatta ggagttgagg catttaacag
    66781 aggtctagga gattttagta taaacatgtt tatgacagta ttagagatgg atagagatga
    66841 tattgactca atatataata taacaagtgt attatgtgaa ttagaagaat ttgatactgc
    66901 ttataattta attaaaaata gtagcgaaaa agtacagagt gaaagtgata tacaagagat
    66961 actttgtttt attgagggga agaaatatga ataatgataa gatatgtttt ataacctgtg
    67021 taaatgatga aaggctttat gaagaatgtc ttgtatatat aaatagccta gacattccta
    67081 aaaactttag tatagaaaca gtagctttaa gaggagccga ttctattgcc tctgcttata
    67141 ataaagctat gagagaaaca gattctaagt ataaggtata cttacatcaa gatactttta
    67201 taattaataa aaatttcata catgatattt tagagatatt taaaaaaaat acagatgtcg
    67261 gaatgattgg tgtagctgga gtaaaagata tgcctgtgaa tggaatttgg tgggattctg
    67321 ataaaaaagc agggaaagta tatgatagtc atacaggtaa aatggatatt tatgaattta
    67381 gtaactttga agaatgtttt aatgaagtta tggctataga tggattaatt atgattactc
    67441 aatatgatgt attttggaga gaggataagt ttgatgggtg gcatttttat gatttgtcac
    67501 agagtatgga gtttagacaa aataaataca aagttattgt accaaaacaa aataagcctt
    67561 ggtgtatgca tgattctgga gtagtaaata taaataatgg atatgatgaa tataggataa
    67621 aatttgtaaa agagtacata aatcttgatt tatatagaga aaatttttat gaatttgata
    67681 agaagaaagc aagtaccata cttaagagta ataagcatgt aatggattgt atgaaagagg
    67741 agcttacaga acaattaaga ttaaagaatt acaaggatgt tttaggtatt gtttctaaaa
    67801 ttgctcattt ttctgttttt aatcatactg gtttgtattc ttgtgaagaa tgtgagagag
    67861 cattgctaga atgtgcaaat aatttaccag aaatagaaaa tacgaaatta tttacaaaaa
    67921 aagatttaaa aagaagaaaa gtattacatg ttttaagtga aggatattca acaggaggtc
    67981 atacaagact agttaaaaat tggattgaaa ttgacaaaga ctctgttcat tcagttgtaa
    68041 ctacatggca atcagataca ctgccagact ggttattaaa agcaataaat gatagtggtg
    68101 gcgaagttat atctttggat ttaatttcag atgattattt aaaaagagct gtagctttga
    68161 ggaaaatagc ttatgactgg gctgatatag ttattttata tgttcatatg tgggatacaa
    68221 tacctgttgt tgcttttgga gttgatggga aaatacctat tttacatgtt aatcactatg
    68281 atagtacttt ttggttaggg acaagtattg cagatattgt cattgatttt agcgaaacta
    68341 gtcaattaat tacattagat aaaagaggca tagataaatc tgaaatatta ccaatgccat
    68401 taaaagaaaa agaagtattt tcaaaatctg aagttagaaa acgatataat ttagaggatg
    68461 atgtgacaat aatattgact attgctagtg aaagaaaata caatagtgta aatgatatta
    68521 attactttaa aattgctaaa acattgattg aaaatacaga taatacaatt attattgttg
    68581 taggagttga tacagagaat ctctattgga aaactttaag taatgttaca gataatagag
    68641 ttatatgttt tggaacaaca gcaaatatag atgattttta taagatttcg gatatatacc
    68701 tagagtcttt catggtagga agtcatactt caaaattaga tgcaatttta tatgatttaa
    68761 ttcctgttaa gtttaaaaat gaagtttcac caatttttac aaatatgtgg aattttatag
    68821 ataagtttga ttataagaat ataaaagagg ttatagacat gataagaagg tacaatacta
    68881 gtgaatttga cagggataag ataaaagcaa aacaatcgga aataaaacat tttgttatag
    68941 ataaacattg tattgaaata aaaaactata tagacaaaat atataatgaa gttaatcatc
    69001 ataaagttaa gtatgattta tataaaaatg atgaaatcga ggactatcag ctttttgtag
    69061 cattatatgg ctatatggta aaaaataaaa acatgtcata ggtggtaaaa tgaacaatat
    69121 tcatatcaat aaatttgaaa aaagctgtga aaaaaactgg gattattttg tagaaaataa
    69181 atctataaat ggtacatttc tgcaaactag aaatttcctt aattatcatg aggagggaaa
    69241 attcactgac cactcattaa taatatgtaa aggtgaagaa atactagcag tcgtacctgc
    69301 atgtgaaact tatgaagatg gtgaaagaat atttttttct catttaggga gtacttttgg
    69361 tggcattata ataagaagtg gttttaatga tattaggcat gtaaatgcca ttacaaataa
    69421 gttagaaata tatttacagg aacaaaattt cgataaagta tatttaaaaa atacatcaag
    69481 aatattttgt aaagaggata cttcattact agaatatttt ttatttaaga ataattacat
    69541 gttttatgat gaattaagta tgtttttaga attaagtaag tataaagaag atatattagc
    69601 caattttaaa tacagtaaaa gaagagattt taaatattct ttgaagaata atcttacctt
    69661 taaaagactg tatagtgatg aagatatagt ttgttttttt aatttacttt ctaattgttt
    69721 agatagtaaa ggagctaaag taatacattc agtagaagaa cttttggagt ttaaaaataa
    69781 tagattaaaa gatattgtag aattttatgc agtatatatt gatgacatta tgatttgtgg
    69841 gagcatggta tttaactttc aagaaagagt ttatcatact caatatctag catcaaattc
    69901 gacatataat aaatattatc caatgatatt tttatactat aatctaataa aagatgcttt
    69961 agagagaaat tttaagtatc tttcttttgg aattagcact ctaaatagag gcaaatactt
    70021 agatgagggt ttagctacat ttaaagaagg ttttggttca agtgcaattt gtaacagaac
    70081 atactataaa aatataaata agtgagaatc atgttttata gagtttttag agtatagtaa
    70141 atatctagta ctataatatt acataaaaat aattagttta taaagaaggt ttgtatatga
    70201 aaagattaat gattttaggc tcaattaagt attttgagaa tattgttatg tcagctaaaa
    70261 gagagggaat atacacaatt gtttgtgata atagaattga tactcctgcg aaaaagattt
    70321 gtgatgaagc aatagatgtt gatgtatttg attttgataa aataaagaat atagctatag
    70381 ataaaaagat agatggaatt ttaactggat ttacagattc tttaatgaag ccatatgttt
    70441 atattgcaaa tgaactaaat ttgccatgtg tgatttcttg caatcaactt gaatctgtaa
    70501 ctaataaggc tgttatgaaa aattatttta aaaataatgg aatacctaca actgattatt
    70561 gtattattga aagtttgaaa gatttagaaa aaatacaagg attaaatttt ccacttgtaa
    70621 taaaacctgt tgatggttat ggttcaagag gtgtatattt tgtatcaaac atagaagaat
    70681 taatagaaaa atttgaatat tctaaggaag cctcgcaaga tggacaagta ataattgagg
    70741 aattttatga aagtgaggaa atacaaggac tagcttgggt tcataatgga gagtcacatg
    70801 tattttatat tggagataga gagcttgtaa atatacatac aggaagagct ggaaaaccag
    70861 atagattaat ttatccatca aagtattgtt ttcaatatga agaagaaata aaatccatat
    70921 atcaaaatat aacgcaggct tttggcataa aaaatggacc tctttatgta caaatgttag
    70981 tgggagcata tggaataaag gtatctgaag taatgccaag attacctgga ggttgtgact
    71041 atatggctat ttctcaaata acagatttag acataggaag tttatttgca aactttagtg
    71101 tgaataatga agtaaatttt gatgaaataa agaaatataa tatgcatcta tcaaaatgtg
    71161 tatatgcatt accaatatac ataagacctg gcacaataaa aaagataaat aatatagata
    71221 aaatagaaaa cttagattat gtcactcaat ttctattact agtaaaagaa ggagatacaa
    71281 taggattata tgaagatgag agacaggatt gtggaagagt ttatggagta gcaaataatg
    71341 tatttgatgc aaatataaag aagaaatgta ttcatgaaat gatagaaata cttgatgaaa
    71401 ataatgaaaa tatgatagaa aatttttaaa taagggagta tgatagatat gaagctaata
    71461 actcatgaac aaattatgtc attaaacatt tctcagaaac aaggatacga gtggataaaa
    71521 gaatcctttt taacaaaaaa agattgtcaa ttaccaccaa aaataagcat ggtagaagaa
    71581 gggcatgtat tttacaatgt aatgccgtcc ctaaatcata atcataagat tggtggaata
    71641 aaaatggtta caagattgcc agggagaaat ccaagtttaa attcagagat atttctatat
    71701 aatatggaag atgctaaatt aaaggctatt ttagatggca attatattac agcttttagg
    71761 actgctgctg ttgctgcctt ggctatcgat acatttgcta aaaaagattt taatactatt
    71821 gctattatgg gtttaggtgt aactgcttct gctataatga atatgctttc atatactctt
    71881 aaagataaaa atataaatat aaagttactt aaatataaag accaagcaga gtcgtttgta
    71941 aaaaagtatg aaaaaaatga acaatttagt tttgaaatat gtgacacaca tgaagaactt
    72001 attgtagatt cagatgtagt tatttctact gtgacatata cagatgaata tttatcagaa
    72061 tttaaatggt ttaaaaaagg tgctttatta ttacctgtac atgttatggg atttggaaat
    72121 gttgattatc tatttgataa agtatatttg gatgattata atcatttgaa acatctagaa
    72181 aatatagata gatataaata ttcttgtgaa ttttatgatg ttttaagtaa acaagctaaa
    72241 ggaagagaaa atgatgatga gagaataata tcttacaata taggtttgtc aatacatgat
    72301 atattatttg caaataaaat ttacgaattg tgtgaaacac taagctgtga agaaatagaa
    72361 ttaaaagaac caaaagaaaa atattggata tagtttattt cattcatatt tacaatagtt
    72421 tattgtaaat atgaatatga agtaatatat tgttagaaaa ttagcatgaa taggatagat
    72481 aaatttatgg ataaatataa tttaattagg ttcgtatcta ttggaaacga atatatagga
    72541 aatttagttg cattagaatc aaataaagca ataccttttg atataaagag agtttattac
    72601 atatatgatg taccaaacaa tgtaaagaga ggatttcatg cacacaaaaa cttggaacaa
    72661 gtactagttt gcctaaatgg aagtgtgaag ataaaatgtt atgatggtgt aaaagagact
    72721 atatttaatt tacataaaaa tgatgttgga ttatatatag gaacgggagt gtggcatgag
    72781 atgtttgatt ttgatgaaaa aactgtttta atttctatag catctgaaca ttataatgaa
    72841 gaagattata ttagagacta taaagaattt ttaaagtatt taaataatgt gtagttatta
    72901 tacaattagt gatgtatttt cttaattaaa taaatttaag atagccacat agagaataga
    72961 tagattagga tgtgaattta ttgttggtta aatttaataa tttattaaaa cagttccaaa
    73021 ctcataaaga agaatatgaa cagaagatta aggatgtaat ggaaagtgga tattatatcc
    73081 ttggaaatga agtaaagcat tttgaagaag aatttgctaa ctatgtaggt agcaaatatt
    73141 gtgttggttt agccagtggt ttagatgcac tgataattgc ttttagagca ttaggaataa
    73201 gcaaaggtga tgaagtaatc gtgcaatcaa atacatatat tgctagtgtt atgggaatca
    73261 gtataaatgg agcaacacca gtatttgtag aaccaaatga atattataat atagattcat
    73321 ctaagataga agaaaagata aatagcaaaa caaaagctat attagtagtg catttatatg
    73381 gtcaagcaag taatatgaaa gatatacaag aattatgtga taagtataac ttgtatttaa
    73441 ttgaagattg tgctcaatct catggagcta aatatgatgg aaaaatgaca ggaacatttg
    73501 gtgatatagg ttgttttagt ttttatccaa gtaaaaattt gggtgcattt ggagatgcag
    73561 gagctatagt aactagtaat gaggaaatag ctgataaagt gaaaattctt agaaactatg
    73621 gctcagaaaa gcgatattat aataaatttg tcggatataa ttcaagattg gacgaaattc
    73681 aggcaggttt attaagagta aaacttaaat atctaaatga aattgaaatt gaaaaatcaa
    73741 aaatcgtaga tagatatctt aatgaaatta ataatcccaa agtcatactt ccaaagatag
    73801 aaaataattg tactcatgta tggcatttat ttgttttaca agttgaaaaa cgagatacat
    73861 ttatggaata cttaaagaaa agtggtatag agtctataat acattatccg ataccaccac
    73921 atctatctga agcatatgct tatttgggat ataaaaaggg gagttttaat atagctgaga
    73981 actattcaga taaagttgtt agtatacctt tatttattgg tatggaagat gaagaagtta
    74041 gttatataat taaaattata aatgaatatt aaactactca taataatatc aaaaataaat
    74101 tttttatata tcatttattt aaatttcgtt aattgacatt tttaaatatt gatatattgt
    74161 acaaataaaa ttgaaatata tggaaaaata tagaattaag atgagatttg tcttatattt
    74221 ttataaatta aaaatatttt caaagagttt attataaaat tgtaaactat agtgtttata
    74281 atctttgtaa ttattggtaa ttaaatttaa aaaataaaat atttaattat acacaaaaaa
    74341 gatatttaaa aaatttgtta tttattgtat acttaagtta aaactaaata ggcaaatcta
    74401 gagaaatcta gtgacgcaaa gctataggga ctaaggttta tatacataaa ctatgtcagc
    74461 cagttgccaa aaagagtcct aggtgtattg tatacctaag aaaagtctat aatgactgcc
    74521 tttttggcag tcattttgtt atggaaaaat cactttattt cagtagaaaa tacacataat
    74581 aacatatttt accaattata acataaaata aagaaaaatt atctcaaaaa gttactattt
    74641 tacaaaaaag agcaactttt cgaagaaata tttaaataca tattaaaatt tttttatatt
    74701 ttcatgggac ttcattaaaa aaatattagt tttcttacta aattgataca ttattgtatt
    74761 aatgtaatgg aaatgcgatt aatttaaata aataaagaag tcattttttg taaaatagta
    74821 actttttgag ataatttttc
//
LOCUS       DAEMGG010000004        71474 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEMGG010000004 DAEMGG010000000
VERSION     DAEMGG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 71474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.004"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(121..894)
                     /locus_tag="KPK24_000229"
     CDS             complement(121..894)
                     /locus_tag="KPK24_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG2297949.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="KPK24_000230"
     CDS             complement(894..1280)
                     /locus_tag="KPK24_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2297950.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="KPK24_000231"
     CDS             complement(1305..1622)
                     /locus_tag="KPK24_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG2297951.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="KPK24_000232"
     CDS             complement(1634..1894)
                     /locus_tag="KPK24_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG2297952.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="KPK24_000233"
     CDS             complement(1894..2190)
                     /locus_tag="KPK24_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2297953.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KPK24_000234"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KPK24_000234"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG2297954.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KPK24_000235"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KPK24_000235"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG2297955.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKRYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KPK24_000236"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KPK24_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG2297956.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="KPK24_000237"
     CDS             complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="KPK24_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG2297957.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8082..9188)
                     /locus_tag="KPK24_000238"
     CDS             complement(8082..9188)
                     /locus_tag="KPK24_000238"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG2297958.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9430..10752)
                     /gene="dnaA"
                     /locus_tag="KPK24_000239"
     CDS             complement(9430..10752)
                     /gene="dnaA"
                     /locus_tag="KPK24_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG2297959.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11408..11545
                     /gene="rpmH"
                     /locus_tag="KPK24_000240"
     CDS             11408..11545
                     /gene="rpmH"
                     /locus_tag="KPK24_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG2297960.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11623..11967
                     /gene="rnpA"
                     /locus_tag="KPK24_000241"
     CDS             11623..11967
                     /gene="rnpA"
                     /locus_tag="KPK24_000241"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG2297961.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11999..12250
                     /gene="yidD"
                     /locus_tag="KPK24_000242"
     CDS             11999..12250
                     /gene="yidD"
                     /locus_tag="KPK24_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG2297962.1"
                     /translation="MNKIIYILKEISKHLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12282..12989
                     /locus_tag="KPK24_000243"
     CDS             12282..12989
                     /locus_tag="KPK24_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG2297963.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12958..13587
                     /locus_tag="KPK24_000244"
     CDS             12958..13587
                     /locus_tag="KPK24_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG2297964.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13699..15078
                     /gene="mnmE"
                     /locus_tag="KPK24_000245"
     CDS             13699..15078
                     /gene="mnmE"
                     /locus_tag="KPK24_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG2297965.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENVIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15176..17068
                     /gene="mnmG"
                     /locus_tag="KPK24_000246"
     CDS             15176..17068
                     /gene="mnmG"
                     /locus_tag="KPK24_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG2297966.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17061..17780
                     /gene="rsmG"
                     /locus_tag="KPK24_000247"
     CDS             17061..17780
                     /gene="rsmG"
                     /locus_tag="KPK24_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG2297967.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17943..18728
                     /gene="noc"
                     /locus_tag="KPK24_000248"
     CDS             17943..18728
                     /gene="noc"
                     /locus_tag="KPK24_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG2297968.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18863..19636
                     /locus_tag="KPK24_000249"
     CDS             18863..19636
                     /locus_tag="KPK24_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG2297969.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19638..20501
                     /locus_tag="KPK24_000250"
     CDS             19638..20501
                     /locus_tag="KPK24_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG2297970.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20534..21676
                     /locus_tag="KPK24_000251"
     CDS             20534..21676
                     /locus_tag="KPK24_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG2297971.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21810..22385)
                     /locus_tag="KPK24_000252"
     CDS             complement(21810..22385)
                     /locus_tag="KPK24_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG2297972.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22645..23937
                     /locus_tag="KPK24_000253"
     CDS             22645..23937
                     /locus_tag="KPK24_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2297973.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSESKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24042..24644
                     /gene="yedF"
                     /locus_tag="KPK24_000254"
     CDS             24042..24644
                     /gene="yedF"
                     /locus_tag="KPK24_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG2297974.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24717..24980
                     /locus_tag="KPK24_000255"
     CDS             24717..24980
                     /locus_tag="KPK24_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG2297975.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24995..25180
                     /locus_tag="KPK24_000256"
     CDS             24995..25180
                     /locus_tag="KPK24_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG2297976.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25283..26479
                     /locus_tag="KPK24_000257"
     CDS             25283..26479
                     /locus_tag="KPK24_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2297977.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26693..26971
                     /locus_tag="KPK24_000258"
     CDS             26693..26971
                     /locus_tag="KPK24_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG2297978.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27008..27442
                     /gene="ssb"
                     /locus_tag="KPK24_000259"
     CDS             27008..27442
                     /gene="ssb"
                     /locus_tag="KPK24_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2297979.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27461..27688
                     /locus_tag="KPK24_000260"
     CDS             27461..27688
                     /locus_tag="KPK24_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG2297980.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27831..28151
                     /locus_tag="KPK24_000261"
     CDS             27831..28151
                     /locus_tag="KPK24_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG2297981.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28159..29115
                     /locus_tag="KPK24_000262"
     CDS             28159..29115
                     /locus_tag="KPK24_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG2297982.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29126..31120
                     /locus_tag="KPK24_000263"
     CDS             29126..31120
                     /locus_tag="KPK24_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG2297983.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31121..31570
                     /locus_tag="KPK24_000264"
     CDS             31121..31570
                     /locus_tag="KPK24_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG2297984.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31589..32917
                     /gene="dnaB"
                     /locus_tag="KPK24_000265"
     CDS             31589..32917
                     /gene="dnaB"
                     /locus_tag="KPK24_000265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG2297985.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33122..33430
                     /locus_tag="KPK24_000266"
     CDS             33122..33430
                     /locus_tag="KPK24_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2297986.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33512..34801
                     /locus_tag="KPK24_000267"
     CDS             33512..34801
                     /locus_tag="KPK24_000267"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG2297987.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35127..35202
                     /locus_tag="KPK24_000268"
     tRNA            35127..35202
                     /locus_tag="KPK24_000268"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35160..35162,aa:Lys,seq:ttt)
     gene            35209..35283
                     /locus_tag="KPK24_000269"
     tRNA            35209..35283
                     /locus_tag="KPK24_000269"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35242..35244,aa:Glu,seq:ttc)
     gene            35288..35363
                     /locus_tag="KPK24_000270"
     tRNA            35288..35363
                     /locus_tag="KPK24_000270"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35321..35323,aa:Val,seq:tac)
     gene            35397..35471
                     /locus_tag="KPK24_000271"
     tRNA            35397..35471
                     /locus_tag="KPK24_000271"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35429..35431,aa:Thr,seq:tgt)
     gene            complement(35854..36846)
                     /locus_tag="KPK24_000272"
     CDS             complement(35854..36846)
                     /locus_tag="KPK24_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2297988.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36855..37913)
                     /locus_tag="KPK24_000273"
     CDS             complement(36855..37913)
                     /locus_tag="KPK24_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG2297989.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38038..39501
                     /locus_tag="KPK24_000274"
     CDS             38038..39501
                     /locus_tag="KPK24_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG2297990.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39603..40400
                     /locus_tag="KPK24_000275"
     CDS             39603..40400
                     /locus_tag="KPK24_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2297991.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40413..42515
                     /locus_tag="KPK24_000276"
     CDS             40413..42515
                     /locus_tag="KPK24_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG2297992.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42583..43062
                     /gene="rlmH"
                     /locus_tag="KPK24_000277"
     CDS             42583..43062
                     /gene="rlmH"
                     /locus_tag="KPK24_000277"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG2297993.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43211..43522
                     /locus_tag="KPK24_000278"
     CDS             43211..43522
                     /locus_tag="KPK24_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2297994.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43570..43887
                     /locus_tag="KPK24_000279"
     CDS             43570..43887
                     /locus_tag="KPK24_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2297995.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44100..44801
                     /locus_tag="KPK24_000280"
     CDS             44100..44801
                     /locus_tag="KPK24_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2297996.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45025..46326
                     /locus_tag="KPK24_000281"
     CDS             45025..46326
                     /locus_tag="KPK24_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2297997.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46463..47557
                     /locus_tag="KPK24_000282"
     CDS             46463..47557
                     /locus_tag="KPK24_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG2297998.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIMLLDFIEPWSKFKLILEGE"
     gene            47559..48692
                     /locus_tag="KPK24_000283"
     CDS             47559..48692
                     /locus_tag="KPK24_000283"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG2297999.1"
                     /translation="MKIDILIKGGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48710..49819
                     /locus_tag="KPK24_000284"
     CDS             48710..49819
                     /locus_tag="KPK24_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG2298000.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49884..50624
                     /locus_tag="KPK24_000285"
     CDS             49884..50624
                     /locus_tag="KPK24_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG2298001.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGYVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50629..51621
                     /locus_tag="KPK24_000286"
     CDS             50629..51621
                     /locus_tag="KPK24_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG2298002.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVLICGIAMQLTDSIKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51809..52690)
                     /locus_tag="KPK24_000287"
     CDS             complement(51809..52690)
                     /locus_tag="KPK24_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2298003.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52963..54351
                     /locus_tag="KPK24_000288"
     CDS             52963..54351
                     /locus_tag="KPK24_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298004.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54379..55332
                     /locus_tag="KPK24_000289"
     CDS             54379..55332
                     /locus_tag="KPK24_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG2298005.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            55392..55616
                     /locus_tag="KPK24_000290"
     CDS             55392..55616
                     /locus_tag="KPK24_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298006.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55609..56121
                     /locus_tag="KPK24_000291"
     CDS             55609..56121
                     /locus_tag="KPK24_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG2298007.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQEDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56153..57007
                     /locus_tag="KPK24_000292"
     CDS             56153..57007
                     /locus_tag="KPK24_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG2298008.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57541..58335
                     /locus_tag="KPK24_000293"
     CDS             57541..58335
                     /locus_tag="KPK24_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2298009.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58439..59245
                     /locus_tag="KPK24_000294"
     CDS             58439..59245
                     /locus_tag="KPK24_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2298010.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SITVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     AIHQLFNFLIGIINEDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            59307..59828
                     /locus_tag="KPK24_000295"
     CDS             59307..59828
                     /locus_tag="KPK24_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2298011.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            60243..62330
                     /locus_tag="KPK24_000296"
     CDS             60243..62330
                     /locus_tag="KPK24_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2298012.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            62345..62632
                     /locus_tag="KPK24_000297"
     CDS             62345..62632
                     /locus_tag="KPK24_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2298013.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            62659..63930
                     /locus_tag="KPK24_000298"
     CDS             62659..63930
                     /locus_tag="KPK24_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG2298014.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            63988..64587
                     /locus_tag="KPK24_000299"
     CDS             63988..64587
                     /locus_tag="KPK24_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2298015.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            64592..65497
                     /gene="dapA"
                     /locus_tag="KPK24_000300"
     CDS             64592..65497
                     /gene="dapA"
                     /locus_tag="KPK24_000300"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG2298016.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPSKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            65603..66235
                     /locus_tag="KPK24_000301"
     CDS             65603..66235
                     /locus_tag="KPK24_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG2298017.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            66713..69220
                     /locus_tag="KPK24_000302"
     CDS             66713..69220
                     /locus_tag="KPK24_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2298018.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            69562..>71474
                     /locus_tag="KPK24_000303"
     CDS             69562..>71474
                     /locus_tag="KPK24_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298019.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNETTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRALNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYE"
BASE COUNT        27246 a         8528 c        11932 g        23768 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ctttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa atacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttaacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat ctctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 acaatacttt tattttagtt ccttctattt caactatatc tcctgatttt atcttttttc
     7801 ctcttcttaa ttctatttca tcatttactg tcactaaacc ttcttgaatt aaaaattttg
     7861 catgacctcc tgttgatgca atctctgcta atttaagaaa ttgatccaac tttatatatt
     7921 ctgattctat agttatttca gtcataatag ttcctccttg tcactatttt aaacaaattc
     7981 ctattgatac actaaaagta tattatacca taaatatgct tataaaagga gttgtctaag
     8041 attagaaaaa ttttctacaa aagacaactc cttcatgtct gctatatatt tgatgatatt
     8101 cttactggaa gcagtaagta aatataatta acatcatctg ttggtttaat tatacaagga
     8161 tttacatttg tagtaaattc tataaatatt tcctcattat ctatattttt caaaccttcg
     8221 ataaaatatc tagaattgaa agctatatct aggtaatctc catctaaatc tatttcaact
     8281 tcttcataca catttccttt ttctgtatta gaagtaattg ccattacttt atctcttata
     8341 gataacttta taagattatt tttttctgat tgagacaata aagacgctct ctctatacta
     8401 tttaaaagtt ccttagtatt taatttaact ctgctgttat gttctcttgg caataatttt
     8461 ttataatcaa taaaatctcc ttcaagcaat cttgtaataa ttttcgtatc attaattata
     8521 aaaatagcat ttttttcgtt aaatccaact ttaacattat cctctccaga aagcaaacta
     8581 tttacatcta taagtgtctt tccaggaatt atgactttta tattttcagt taaactatca
     8641 accgaacaac tttttacagc taatctatat ccatcaattg ctactaaatt taagtttcta
     8701 tctacaattt ctaaaagttc tcccattaaa acaggttttg tttggtcctg tgagatagca
     8761 aatacagttt gtttaatcat attttttagg atttcttgag gtatgctata taaatcttct
     8821 tcatttaatt ctggtaattt aggaaattct tttgctgcat aacctttaat tttaaatctt
     8881 gaatttacac aatttatgta aatattattt tctgaatcag tttctatctc aacaaaggaa
     8941 tctggtaatt ttctaattat atctccaaat agccttgcat ctacaacaac atcacctttt
     9001 tctatgattt ctgcttgtac ataagtttct attcctattt cagcatcata tccagtaagt
     9061 tttaattgac cttcttctgt agatataagt atacctttta ataattctat tgttgttttt
     9121 ccattaatag ctttttgagc aattccaatt ctatttgcta ggattttttg attacaaatt
     9181 attttcaatg tggataacct cctctggctt tttatatata aaaataagtt aaaatcatag
     9241 tagtaatagt agtaggtgtt gttgataatg ttaataagta tgtataacat tataaataca
     9301 aagatattaa catgtgtata agatgttaat atctttgtta ttttagactt gataatttgt
     9361 taataatttt tgttcaaaaa aagtattaac aggttattca agagatataa acatacaatt
     9421 gtgtataaat tatcccttca aatctgatat aattttgtct attttagtct taatttcttc
     9481 actttcttct atatccttag atactttgtc atgagcatga attactgttg tatggtctct
     9541 acccccaaac tcttcaccta ttttaggaag ggataaatca gtaagctcgc gcgttaaata
     9601 catagctatc tgtcttggat aagctattga acgtgttctt tttttagaat taaaatcttc
     9661 catctttaaa ttgaatacag aagatacttt ttcttttatt ctgagaacat ttatttcttc
     9721 atttttagaa gttgttataa tatcttttaa agcttctgta gcaagatcaa aagatattac
     9781 cctattgcta agagaagaat aagcaaccac tctagttaat gcaccttcta gttcccttat
     9841 gtttgactta atattttttg ctatatatga cataacctca tttggcacat ctatacgttc
     9901 tagttgagcc ttttttctaa gtatagcaat tcttgtctca aagtcaggtg cttgaatatc
     9961 agttataagt cccatttcaa atctcgatct tagcctatct tctagtgtag gtatgtcttt
    10021 tggcggtcta tcactagata taattatttg tttgtttgct tcatgaagag tattgaatgt
    10081 atggaaaaac tcttcttgag ttctttcttt tcctgctata aattgaatat cgtcgattag
    10141 taatacatct acgtttctat atttattcct aaattcttcg tttttatcgt cttttataga
    10201 gtttataagt tcattagtaa atttttctga agaaacgtaa actacttttg agtctttttt
    10261 ttgactaaca atatgatgac ctatagcatg cattaggtga gtcttaccta atccaactcc
    10321 tccatacaaa aaaagtgggt tataagcttt agctggagat tctgcaactg caacgcatgc
    10381 agcatgtgca aatctattac tattaccaat aacgaatgta tcaaatgtgt attttggatt
    10441 tagattgggg taaagaactc tataatttaa atctgtgcta tcagaattta aatcagcaac
    10501 ctctttttcg ctcaatacaa atttaatatt atattttttt aatgacagtt gactaatagc
    10561 atcttcaatt aaatgtaaat atctattctc taagatatct ttattaaaat cactaggagc
    10621 cagtaagatt aaatcattaa gatgtatttt taatggtacg atgtttttaa aaaaggtatt
    10681 aaaacttact gaagttaagt caccttttat taattgtagg gttttgtccc ataaagaaac
    10741 tatatccata atataaacct ccgaacagaa taattaatat tttccacaaa gttattaaca
    10801 gaaaagcaaa aatatatata aattttacag aataactaaa tttatggata atggtgttga
    10861 taaattaata atagtataaa ttattaaaaa aatatgaaaa gatataaaca atatgtagat
    10921 aaagttataa ataacataaa catacaataa atggtgatta taactagagt attgtcagta
    10981 atcttaaaag ttatacacaa aagtatccac aagctgtgaa taatttctaa taagttgata
    11041 acatttatgc acattatcaa caagtatatt tataatatca aaatatatgt caaacttcaa
    11101 tgatttgtaa ctaatttatc cacaatatta actaattgtt gataaaaata taaacatgtt
    11161 aaaattactt tatttgtgta tataattgaa aaaaataatt tagaataaaa ttaaatagat
    11221 ttttactagt attttcaata aaaaaaatac ataatatata tatggtagat tttgtgatat
    11281 aagcgtccca aaaactcgtt gacaacagta gtaaaagtat ttataattat atgtgttatg
    11341 tttactttga gggtaaagtt attattttta taaacggaaa ataaaatctg aaaggcggtg
    11401 caaaataatg agtaaaagaa cttatcaacc aaaaaagaga caaagaagta aagaacatgg
    11461 tttcagaaaa agaatgaaga cgtctaacgg aagaaacgtg ttaaagagaa gaagagctaa
    11521 aggaagaaat agattaactc attaattaca aaagggccgc tgcaaaggtg gcctttttgt
    11581 gtaaataatt acaattcata gctgtgttta tggaggttaa ctatggactt taataggact
    11641 aaagggttga aaaaagactc tgattttaga aaagtatata aacacggcaa atcttttgca
    11701 aacaaatatt tagtaatata tatacttaaa aataaatcag attatagtag agtaggtatt
    11761 tctgtttcaa aaaaagtagg aaaagctatt actaggaata gagtaagaag attaataaaa
    11821 gaagcctaca gattaaatat tgatgaaaaa attaagcctg gatatgatat agtatttata
    11881 gcaagggtat ctagtaagga tgctactttt aaagacatag acaagtctat aaaaaattta
    11941 gttaaaagaa cggatatttc tatttaattg tatgctataa atatggaggt taatgacttt
    12001 gaataaaata atttatatat taaaggaaat aagtaagcat ttatcgaatt tatgtattta
    12061 tttagtgaga ttttatcaaa agtatatatc tcccttaaag ggacctacct gtagatttta
    12121 tccaacttgc tcacaatatt caatagaggc gtttaagaaa tatggatttg taaaaggtat
    12181 gtatttaacg ataagaagac ttttaaaatg tcatcctttt catccaggag gttacgaccc
    12241 tctaaaataa aagagagtaa ctattaggag gtataaaaaa attgaatata ataggaaatt
    12301 tcttaggatt gattcttaag gtaatatttg aatttgttaa tcattatggt atttctataa
    12361 tactttttac gatacttgta aagattattt tattaccatt aacaattaag cagactaagt
    12421 caacaaaggc aatgcaagat atacaacctc gtataaaaga gatacaagaa aaatataaaa
    12481 ataagccaga aaaacaaaat gaagaaattg ttaagttata tggagaagct aaaattaatc
    12541 cactatctgg ttgtttacct ttattaatac agtttcctat attaatagga ttatttagcg
    12601 ttttgagaga accagttgct catggcgttt ttgcaaacaa agctgcattt ttagcagctg
    12661 acaatgggtt tttatggata aaaagcttaa catcaccaga ttatgtatta gcagtatttt
    12721 caggagcaag tgcatatgtc atgcaaaaag taatgacacc aaaagatcag ttacaaggct
    12781 ctatgaaagt aatgacatat gtaatggcag gtatgtcttt ctattgggga tttatattcc
    12841 cagcaggact tacattgtac tggacagtaa gtaacttatt ctcaatagct caatattact
    12901 taataatgaa tcctttaaaa gctaaactgg ctgctaactc taaggaggaa aaagttaatg
    12961 agaaaaagcc taaaattaat aaaaagtaag agtaaagagg aagctataag taaagctata
    13021 gcagagttga atttaaaagc agaggatata gaagtagaaa tacttgaaaa tccaagtaaa
    13081 gggtttttag gttttatagg tgctaaagat ggtacttatg aaatttttgt tatcgagagg
    13141 gaagtagatg tagctaaaaa ttttatagag gtaatgttga aaaatgcaaa tgttgatgcc
    13201 aaagtcaatg tatctcaaaa agataattta atcatggttg atattgaagg caaagaagct
    13261 gcaagtttaa ttggtagaag aggggaaact ttagattcta ttcaattttt gactggatta
    13321 gctcttaata aaataaataa agattctcat acaagagtac ttgtagatat agaaaattat
    13381 agatcaaaac gagaagaatc tttaataaga tatgctaata aagttgctag agaagtagcg
    13441 aaaacaagaa aaactaaaaa attagattat atgaatccat atgagagaag aataatacat
    13501 tcagctcttc aaaatgacaa atatgtaatt acatatagtg aaggaaccga tccatataga
    13561 agattagtaa ttgagtgtaa aagataaagt caacctccta aacattaggg ggttttattt
    13621 tttaatatta ttaaatcatt aatggttggt taaatgtaaa tctatatgta aaaaattatg
    13681 aattaagagg tgataaattt gtttatagat gatacaattg cagcaatagc tacagcacct
    13741 ggagaaggtg gtataggtat acttagaata agtggagaaa aagctttgaa ggttgcagaa
    13801 gaaatcttta aatctatgtc tggaaagagt attgaagaat acaataagag aacattaata
    13861 tatggaaata tagtggataa tgaaaatgta attgatgaag ttttattggc atatatgaaa
    13921 ggaccaaatt cctatacagg agaagatgtt atagaaatta attgccatgg tggatttatt
    13981 tcggtaaaga agatattaga attaatacta tcaaaagatg ttagattagc agaggctggt
    14041 gagtttacta aaagagcatt cttaaatgga agaatagatt tatctcaagc agaggctgta
    14101 attgatgtta taaaagccaa aactgatata gctcatgaag ttgctcaaaa tcaattagag
    14161 ggttctttat caaagaagat aagagaattg agagataaag tcactgagat attagctcat
    14221 gtagaagttg ctatagatta tcctgaggaa gatattgagc atataacata tcaaactctt
    14281 aaagaaaaaa ctgatgaact taaaaaagac attaaaaaat tatatgatac agctgaaagt
    14341 ggaaaaatac ttagagaagg cttaaagacg gtaattgttg ggaaaccaaa tgtaggtaaa
    14401 tcttcactac ttaattctat tttaggagag aatagagcaa tagttactga tataccagga
    14461 actactagag atgttataga agagtttgta aacataaaag gtataccttt aaaaattgtt
    14521 gacacagcag gtataagaga tactgatgat attgttgaaa aaataggtgt tgaaaagtct
    14581 aaagaatcat ttactagtgc agatttgata gtaatggttt tagatgcatc tagaaagttg
    14641 tcagaagaag atatagagat attagaaaaa cttaaggata aacaaacaat agtgctgtta
    14701 aataagaatg atttgaaaca agagattgaa gaagaaaaaa tacttaaata tgtagaaaat
    14761 aatagtataa taaaaatatc agctttacaa caagaaggaa tagaagaatt gcaagataaa
    14821 atagagtcta tggtatataa aggtagtatc aaaaataatt ctagtttagt agtaacaaat
    14881 tcaagacata aagatgcact tagtaaagca tataaatctg caacagatgc tttaattgct
    14941 ttggaacaaa gtatgccatt tgactttgta gaagtcgatt taaaaaatat atgggactat
    15001 cttggatata ttaatggtga tacagtaaca gaggatttac tagataatat attccataat
    15061 ttctgtattg gtaagtaatg tacaaaagta aacttgtttt tattatagag atttttaaaa
    15121 aatttgaagt gtttctcatg aaacattaat tttgaaaaga aaggaattat aaatgataaa
    15181 gtttgaagca ggaaaatatg atgttatagt agtcggagca ggacatgctg gatgtgaagc
    15241 tgctttggct actgcaagaa tgggatataa aactttgata ataacaatgt ctttggattc
    15301 catagcatta atgccttgta atccatcaat tggagggact ggtaaaggtc aattagttaa
    15361 agaaatagat gccttaggcg gtcagatggg attaaatata gacaagacat atatacaaag
    15421 tagaatgtta aatactgcca aaggtccagc ggtacactct ttaagagcac aagcagataa
    15481 atttaaatat catgaagaaa tgaaaaagac attagaagat gaaccaaatc ttgatatagc
    15541 tatggatgaa gttgtagaaa tacttcatga aggaaatgtt gtaataggag ttggaactaa
    15601 actaggatgt tcatttaaat ctaaagctgt aatattggcc actggagttt atctgaactc
    15661 aaagatatat atgggtgaag ttgcatttta tgaaggtcct aatgcattag ggtatgcaaa
    15721 atatcttaca gatagcttag ttgagcttgg ccttagaatg agaagattta agacgggaac
    15781 tccagcaaga gttcatagag atagtataga cttctctgta atgtctttac aagagggtga
    15841 tgaaaaagta actccattct catttatgaa tgaaaatata gaaaaaaaac aagaaccttg
    15901 ttatttaaca agaactacag aagaaactca aaaagttata ctggataatt taaaaaggtc
    15961 tgctatgtat agtggagtaa tagaaagtac tggtccaaga tactgtcctt ctatagaaga
    16021 taaagtagta agatttagtg ataaaacatc acatcaatta tttatagaac cagaagggtt
    16081 aaatactaaa gaaatgtata tacaaggaat ttcaactagt ttaccttttg aagttcaatt
    16141 agatatgtac aaaactataa aaggacttga aaattgtaag ataatgagac ctgcatatgc
    16201 tattgaatat gattgtgttg acccaactca actaaaaata agtttggaaa taaagggtgt
    16261 tgagaactta tttagtgcag ggcaatttaa tggaacttct ggatacgagg aagcagcagc
    16321 tcaagggctt atggctggta taaatgcagt tagaaagata gaaggaaaag agccatttgt
    16381 attagaccgt tcagaagctt atataggtgt cttattagat gaccttgtta ctaaaggaac
    16441 taacgagcct tacagaatga tgacatcaag agcagaatat cgactatatt taagacaaga
    16501 taatgcagat atgagattaa ctcaaaaagg ttatgatata ggattagtta aaaaagatag
    16561 atatgaaagg ttcctaaata aaaaggctgc tgtagagaaa gaatttgaaa gactaaaaaa
    16621 tgaaagagtt acaccaaaag aagtaaatag tttgttggaa gaaaaaggag caacaccaat
    16681 aaaggttgga atatctctat atgaattttt aaagagacca gaggttactt acgaattgct
    16741 tgaagaatta gggaaaggcg ctggagaaga tgtttcaaga gaagttaaag aacagtgtgt
    16801 aataataact aaatatgaag gttatataga gaaacaattg aaacaaatag accaatttaa
    16861 aaaacttgag aataaaaagt tggatgagaa aataaattat tcatctatag aaggtcttag
    16921 attagaagct agacagaaat tagatgatat aaaacctatt tcaatagggc aagcatctcg
    16981 tatatctggt gtttctccag ctgatatatc agttttactt atatacctag aacaaatcag
    17041 aagaactaga ggtggcaaag gtgaataata tagaacttct gaaaaacggg attgaaggtt
    17101 ttaatataga tacgaatgac agtatgttag aaaaatttaa aatctataga gagatattag
    17161 ttgaatggaa taaaaagatg aatcttactg gaatagaaga tgaaaaagaa gtgtatataa
    17221 aacatttttt agactctatt tcagctgtaa aaaatggata tataaaaaat gggatgtcta
    17281 taatagatgt tggtacaggt gctggttttc ctggaatacc attgaaaata tgtttagata
    17341 gcttagaact tactttgtta gattcgttaa ataaaagaat aaacttttta gaggaagtta
    17401 gtagagtact agagttagat aatattacat ttatacatgg aagagctgaa gattttggta
    17461 aaaatgaaga ctatagggaa aaatatgatg ttgctacggc aagagctgtt gctggattac
    17521 cgattcttat ggaattttgt gtaccatttg taaaagttgg agggtatttt atttgcttaa
    17581 aaggtccgaa tgctaattta gagctagaag aatcacaaaa ggctattgat gttttaggat
    17641 tggagtatat tgaaaaaata gatgtagagt taccagaggt agatttaaat cataatatat
    17701 tagtttttaa aaaagtgatg gaaacaccag ttaagtaccc tagaaaagct ggtaaaccag
    17761 ccaaaaatcc tattaagtaa tgaaaaattt attattggta agcttaatta tttctcagga
    17821 aataattaag cttactgtaa aaaaacaaaa ataaaggaat tattaaagta gatgcagaaa
    17881 taaatttgta tgtacatata gagaacaaaa ataatttata tctaaaataa gagaggtatg
    17941 ttatggaaga taaaagagtt atggagatac ctatagaaga tgtagttcca aatccatatc
    18001 aaccaagaaa aatattttca caagtttcat tggaggaatt gagcaattct ataaaggtat
    18061 atggtataat tcaacctata acagttagag caaaagatgg caaatatgaa ctaattgcag
    18121 gagaaagaag attaagagca gctaaattag cagagcttaa aaccatacca gcaattataa
    18181 ataatatgaa cgatgaatct tctgctgttc tagcgttatt agaaaatttg caacgagaag
    18241 acttaaattt cattgaagaa gcaattggtt atgaaaactt aataaaagaa catgctttta
    18301 ctcaacagca attagcagaa aaattgggca aaaatcaatc tactgtagca aataagctta
    18361 gaatattaaa gcttccaaac gatataaaaa tgaagctaat agagaataat ttaacagaaa
    18421 gacatgctag agcatttctt aaactaccaa gtgaagattt aatgcaaagt gtgttagaca
    18481 aagtcataaa aaatgaattg actgttaaaa aaacagaaaa attgatacaa gatgtattag
    18541 aagaaactaa agttcaagaa gagcctgata aaaaacagaa tataaagggt gctatgagta
    18601 taagaattta cataaatact ataaagcagg cctttgatgc tatatcaaat acaggaattg
    18661 atgctaagta taatgaaata gataagggag attatatgga agttgttgta aaaatcccta
    18721 aaaaatagag attactgctt accagtaatc tttttttgta atataagcct tgtaatataa
    18781 attgatttat agacattatt tatacttaaa aaacacctat tcaacattta aaattaattt
    18841 aattcttaaa ggggggaaca tcatgggcaa agttatagct gtattcaatc aaaagggtgg
    18901 agtcgggaaa actacaacaa atgtaaattt aagtgctagc ttaggaactt tgggaaagaa
    18961 aatattagta ttagatttag acccacaagg aaatacaact agtggatatg gtattaataa
    19021 aaacgaagta gaaaatacaa tctatgaaat tatgttagat gggttacata ttaaagaagc
    19081 aataataagt actgaatttg agaatgtaga tgtagttcca tcagctactg aactatcagg
    19141 tgcagagata gagcttacat ctaaaactaa tagagagtat attttaaaaa attccataaa
    19201 agcggtaata gatgaatatg attatatatt tttagattgt ccaccctcac tggggatgct
    19261 tacaataaat tgtttaactg ctgtagatag tgttttaatt ccaatacaat gtgaatatta
    19321 tgctttagaa ggtgttagtc aattaatgga gactataaaa ttagtaaaat caagattaaa
    19381 tgctgatata gaaattcaag gtgttgtttt aagtatgttt gatggaagag ctaatttatc
    19441 aatacaagta gttgaagaag ttaagaaata ctttaaaggt agtgtatata caactttaat
    19501 tccaagaaat gtaagacttg cagaagctcc aagtcatgga aaacctgtaa tttattatga
    19561 taaaagatgt agagggtctg tagcttattt ggaactagca gaagaattta tagatttaga
    19621 agaggaggaa tggtagtatg gagaataagt ctaaaagaag taatagatta ggtagaggtc
    19681 ttagtgcatt gataccagaa attaaaggag aaacttcaga aaaagaaata gtcaacatag
    19741 atatagacaa aatatatcca aacgaagttc aacctagaaa gcaatttgat gaagaaaaga
    19801 taaaagtatt gtcggattct ataaaaaatt atggtgtttt acagccaata gtggttaaaa
    19861 tggatgaaaa taataaatat atgataatag ctggagaaag aagatttaga gcatcaaaac
    19921 ttgcaaataa aaatcaaata cctgctataa ttaaagatat agatatgaaa gatataatgg
    19981 agatagcctt aattgaaaac ttacagaggg aagatttaaa ttcaatagaa gaggctctag
    20041 catataagag tttaatagag cattacaatg taactcagga agaaatatca gaagctgttg
    20101 ggaagagtag acctcatata actaatacac taagactttt aaatcttgga caagatgtta
    20161 tagaaatgat agacagtggt agaattacag caggccatgg taaggcttta ttgagaattg
    20221 cagataaaga tttgcaactt caaatagcga aaaaaataga agaagaagaa ctttctgtaa
    20281 gagaagtcga aaatatagct aaaaaaatat ctgaaaataa acaagaagaa cctaaaaaat
    20341 ctaaacctaa ggatgtattt attttagatg ttgaagataa acttagaaat atatttggaa
    20401 caaaggtgaa tatttctaaa ggaaagaaaa aaggtaaaat agagatagaa tactataatg
    20461 atgatgattt aaatagtatt gtatcaatgc ttcttgaata gaacaaaaat ataaaacaga
    20521 gaggaagatt gttatgattt atttagataa tgctgcaact acatatccaa aaccagaaag
    20581 agtgtataat gctgttttag attgtatgaa aaattattgt gcaaatccag gtagagcagg
    20641 ccataaactt gctatgagag ctgctaggga gatatatgat acaagagaga atatagctaa
    20701 gttgttcaat gtaagtaatc ctatgaatat agtatttaca tctaatgcaa cagattcttt
    20761 aaatttagcg ataaaaggtg tattacaaga aggagaccat gtaataacaa cgtctatgga
    20821 gcataattca gtaataagac ctataaaagc attggaaaaa aggggtattg aaaatactgt
    20881 agtaaaatgc gattatgagg gatttttaga ttatgaagat ttagaaaaat caataaagaa
    20941 taatactaaa ttgattgtaa caacacatgc gtctaatgta tgtggaactt taatagatat
    21001 aaaaaaagtt ggagaaatag ctaaaaaaca taatatacta tttttagtag atgcatcaca
    21061 aacagcaggt gtttatgata tagacgtaaa tgaatgtaat atagatatgt tggcaatgcc
    21121 aggtcataaa tgcttatttg gacctcaagg aacaggtata ttatatgtaa gagaagggtt
    21181 gaacttaaat atattaaaag aaggtggaac aggtagtaaa tcagaagaaa tagttcaacc
    21241 agagttattc ccagataaat atgaatcagg aactcataat actccaggta tagcaggtct
    21301 taaccaaggt atactattta tatttgaaag aggaatcaac aatataagac aacatgaaga
    21361 agaattatgt caatatatga tagacaaatt agaggaagta ccagatataa agatatatgg
    21421 accaaaggac agtaaaaaaa gagcatcagt aatagctcta aatataggag atatggattc
    21481 tggagaggtt actttcttat tagatagtga ttataatata gcgactagat caggaataca
    21541 ttgttcacca ctagcacata ccactttagg tactttaaaa caaggtgcag taaggttcag
    21601 tataggatat ttcaacacaa aagatgagat agataaagca gtggaagctt tgaaaaaaat
    21661 ttcaaaaaat aaatagcatg agatttaata atatagaaat aaaaaaaggg gatatttcaa
    21721 aataaaaatt tgaatcatct ccttttttgt acaaaaaaga aaaggggttc ctaagaacct
    21781 ctaatctatt tgataatagt ttcaagaaat tactttaagt aatctccttc ttcaagaact
    21841 atatgaccac tttcgtaatt agaaccatca acagtaattt ttatttgttc aacattcata
    21901 ttttttatgt aagtacgagc aactgaatca agcattaaag tttctgcatc actacctaag
    21961 tttgactttg taaagtcaga gctaacatct aatataccag tttttacacc atcaatgtct
    22021 tttgtagtaa atgaatttaa ttttgcagat tctggaacaa ctttaagttt ttgaagttct
    22081 tcaaataaag tgttctcatc aattttattt aaatcaactt tattttcaat taaatctgtc
    22141 ttatctacat caaaagagta aatagtagct gattgagctt tagtttcttc ttttggagta
    22201 ggtttagctt tgttattagg aacctctttt tcagtattgt tttcttcttt tggttgctca
    22261 acatttgtat tgctcttagt ttctttttta gatgaatcac ttccattttg agcattagta
    22321 caaccaactg cgaatataga tatagataat atacttaaca ctaacattat tttcttattt
    22381 ttcataaaaa tttctcctct cttaaaaact attcttatat ataattatag tcgttatgag
    22441 caaatatatg aatacaaaat agtaacaaat ttaaaaaatg atttatttaa attaataaaa
    22501 ctcaaaataa actaaatttt agtcattttt atgggaaatc atttaatgta cagtgaaaat
    22561 attgtaaaat aaaagtagtg tttttaaaat aaatataagt tataattata ttataaaaaa
    22621 atgtcttcaa aaggtgggaa tagtatggaa attttgagct taggagaaaa gattaaaaag
    22681 ttaagaaaag aaaaaaacat gacattgaaa gaattagctg gagataggat aacagcagca
    22741 caaataagcc atatagagag agataaatct catactagtt atgaactgtt ggaatatcta
    22801 ggcgataagc tagatgtaag tgtagaatac ttattagaga ccaaggaaat gcaatcaaaa
    22861 aagataactg ataatttaat actacagagc gaaatttata taaaagaaaa tgaattaaac
    22921 aaggctgagg accaaataaa agaagtttta gatatatgta agagatacga tttaatggag
    22981 aactatggca gatgtaattt tttaatggct aatattaatt tgaaaaaatc taattataat
    23041 gatgctatag aaaactttga aaaggcattg tacttcttta taaaaaatag tgatagtgaa
    23101 aatatactta agtgttattt aaacataggg aaaatatata tgcaggaaga cttttataaa
    23161 ggtgctataa gccactttaa ttttgcagag gaaattttgt cagagagtaa atttgaggat
    23221 gttaacatac acaaggaact atatagtaag atggcatatt gttacataaa actagataat
    23281 ccaaatatgt cattgaagta catagaaaag ataaatgaaa tagataatag aaatgataga
    23341 aaagaagatg tcgatatttt ggtgttaaaa gcaaataata tgcttgagat tggaaaatat
    23401 gaagaatcaa gagattactt taaaaaagca ctaaaaatac ttgagagtga ggataataaa
    23461 actggattag caaatgtgta tttaacaatt tgtgatgttt acagaaaaat aggtgaaaca
    23521 gatagagtac ttgaatattc acaaaaggta tatgacataa agaaaaatga tgaagatgaa
    23581 tacatgatgt caggattatt taagataata gaagcatata tagataaaga agattatgat
    23641 ttagctagaa aatattgtaa gatagcctta gcttcatcaa taaaaaataa aaataaattc
    23701 aatgaatata aagcattgag attctatgct aatatgtata aaaatcagaa tgaagtaact
    23761 cttgcaattg aatatctgat taaatgcata aaaatagtat cagatttagg caacaataga
    23821 atattggcca atttatatat agacttaggt caattatatt caaatatatc aaaagaaaaa
    23881 gagttagaat attatcaaaa aggtgtattt atgtacaaaa atttagaaat aatgtagggt
    23941 aaataaacct ataagggtat agaaacaatc tataacaagc aagataatat ctatgataaa
    24001 atataaattg tatgaatatt tttaaggaga tgaatcagac aatgagtata aaaatagatg
    24061 cgagagggct tgcttgtcct aaaccagtta taaatacaaa aagagaattg gataacctag
    24121 aagaaggtgt tgtagttaca atagttgata atgaaacagc taaggagaat atattaaaac
    24181 ttgctaaatc tttaagttgt gaagcaaata ttgttgacga aaaagagaat tttatatcta
    24241 tagaaattag aaaaggtaaa aatatactag ataaacaaaa tgttgtagat aataaagaaa
    24301 gtgaattaga caatacatgt atatttatat cttcagataa gatgggactt ggaaatgacg
    24361 aattaggaaa agtgttaata aaaggattta tatatacatt aacagaatct aaaccttatc
    24421 ctaagtatgt acttttagtt aatggaggag taaaattaag tgctgaaaat gaagaaacca
    24481 tagaaaatct aaaaatactt gagaatatgg gtgtagaagt tttatcttgt ggtacttgtt
    24541 tagattatta taatttaaaa gacaaattac aagtaggtac agttacaaat atgtatacta
    24601 tagttgagac attaaagaat gcatcaaata ccatttctat ataatgtatg tatgtttaat
    24661 aatataaaac taacaataaa gattaactca aatagatagt cagaaaggat taagtaatga
    24721 atcaaatgta tatagtgtca tttaattcga cacatcatgc gataagatca gaaaagttat
    24781 ttggtgaaaa tagtctaaaa gtaatggctc ttccaacacc aagagaaata acagcaagtt
    24841 gtggaatatc aattaaattt tcttttgaag atatggaaaa gataaaaact attttggttg
    24901 aaaataatgt agatataaaa ggaatttatt gtataagtaa attagaaaat ggttcaaaag
    24961 aagtagagaa gttagattag gaggctaatt atttatgcca atggatttaa aaataggtga
    25021 tattgttgaa ttaaaaaagc aacatgcatg tgggtgtaaa gagtttgaaa tcataagaac
    25081 gggaatggat ataaagataa aatgtactaa atgttcaagg ctaataatgc ttgatagaga
    25141 gaccttagaa aagagagtaa aaaagctaat aaaaaagtga taaaaattta acacttaaaa
    25201 aatgtcaaat accgtatatt aatatccaaa attttggtat aataagaatt gaagaaaaca
    25261 ttttcatggg ggtgctttaa ttatggcagt taaatatgca aaaagaatgc aaggattaca
    25321 aggatcagaa atacgtgagc ttttaaaact tactcaacaa ccacaaataa tatcttttgc
    25381 tggtggaatg ccggctccag agttatttcc agttgaggaa atgaaaaaag tatcagttgc
    25441 agtgcttgaa gaaaatggaa gatcagctat gcaatatact acaacagaag gatatgaacc
    25501 attaagagaa aaaatagcag ctagaatgaa tgataaaaac aaaactaatg taaacaaaga
    25561 tgatatatta gttacaagtg gttctcaaca aggtctagat tttgcaggaa aagtatttat
    25621 tgatgagggt gatgtaattt tatgtgaaag tccatcttac ataggagcta taaacgcatt
    25681 taaatcttat caacctaaat tcatagatgt tccaacagat tctgatggaa tgataatgga
    25741 agaattagaa aagatactag aaacaactga tagaataaaa atgatttatg taatacctga
    25801 cttccaaaat ccaacaggaa gaacttggcc acttgaaaga cgtaaaaaat tcatggaaat
    25861 agtaaataaa tttgaaatac cagtaataga agataatcca tatggagatt taagatttga
    25921 aggagagact ttaccatctt taaaatcaat ggatacaaaa ggattagtaa tattcttagg
    25981 aactttctct aaaatattct gcccaggata cagattagga tggacttgtg cttctccaga
    26041 aattttatct aagtttaact ttgcaaaaca aggtgcagac ttacaagcat caactatatc
    26101 tcaaatggaa gtaagcaaat ttatggatat gtatgaccta gatgcacatg ttgacaaaat
    26161 aaaagcagtt tatgttaaac gtagagatgt aatgcttaag actatggaag aagagttccc
    26221 agaaggatta gtatttactc atccagaagg tggattattt acatgggtag agcttccaag
    26281 taatttaaat gctaaagaat taatgccaaa atgtttagat aagaatgttg cttatgttgc
    26341 tggaggagga ttcttcccta atggtggtag agaaaatact ttcagactta actattcaaa
    26401 tatgccagaa gaaaaaataa tagaaggtat aaaaaatata gcagctgtat taaaagaagc
    26461 tatgggtgta gaagcttaat taaaaataat aacttatata taatacaagg gatggttgtt
    26521 gccatccctt gtttgtttga aaagtatata attcacctta tactaaactt tgtccaaaat
    26581 ttaaattgac agaaataaga ataaatgata aaatgtgtag gtatgagatt ttagtctcat
    26641 ttctctgctc taagagtgta gagccgttaa gtccaaaggg aggtgaatta tagtgagaaa
    26701 ttatgaatta gtttatgtgg taaagccaaa ctctgatgaa gaagtaagag aggctatact
    26761 aaacaaagtt aaggaagttg ttgcaactga cggagaaata gttaaagttg atacttgggg
    26821 aactaaaaaa ttagcttatc caatagctaa gtttacagaa ggtttctatg tattagttaa
    26881 cttcaaatca gcagtagacg ttcctaaaga gatagacaga aacttaaaga taaatgaaaa
    26941 cgtaataaga cacatgatag ttgttgctta ataactggtt ttattataaa aaataggtgg
    27001 tgtttttatg aatcaagttg tattagtggg aaggttgact agagaccctg aattaagata
    27061 catcccaggt acaggaacac cagtggcatc ctttacaata gccattgata gagactatgt
    27121 aaaaaaagat ggatctaaag aaactgattt tatacctgtt gaagtaatgg gtaaatctgc
    27181 tgaattttgt gctaactaca taactaaagg tagattagta gcacttcaag gatctataag
    27241 agtagacaat tatcagacac aatcaggtga aaagagaact tttacaaaag taagtactag
    27301 atcagtacaa gctttggata gtaagaataa atcggaaaat tcatatagag agagtgctcc
    27361 agcttttgag ccaagctttg aacctcaagg tttagaccca caaggtttcc aagctataga
    27421 tgatgacgat ataccatttt aaaaaggagg gaaaaaagtc atgatgaata aaaaaagacg
    27481 taagaaaaaa agagtttgtc aattttgtgc tgacaaaaac gctaaaatag attacaaaag
    27541 tactcaaaga ttacaaaagt atataactga aagaggaaag atattaccaa gaagaatatc
    27601 tggaacttgt gctaagcatc aaagagaatt aacagttgct ataaaaagag cgagaaatat
    27661 agcactttta ccatatacat tagactaaaa aaatgagcct tgtgctcatt ttttattata
    27721 tgtatttata tatctaaatg aggcgagcta taaagctcgt ttttttgttt atatacttaa
    27781 atatattatt atttatgata taatattata tattaaggag ggttgtaaac gtgaaaatag
    27841 ataaaggttc tgagataacg agaaacataa agataataga atggatgaaa acagaaatat
    27901 taatgagtgt aagtgatttg tttaatttat tgtttaaagg ggtaaaacca ttagatgaag
    27961 caattcaaga tactttagca aatataataa tgataacata tctcttagca aagagattag
    28021 gaattagttt tagagatgtg gattataaag tcaaggaaaa aataaaaatt ggtatagatg
    28081 aggaccatag cgttgaaaga tggtatggag atttatctaa tttaaaaaaa catatggata
    28141 atagggagtg atattaaatt gaataataaa attagactat ctaaggcaat gtctataata
    28201 gttttagcta taataatcgc tttagtaata gcctatattc caatgctagg aatatttagt
    28261 atgttagcag ctgttccata tgtaattata ggtgctatta ctgatagaaa gtattctctt
    28321 atatctattt tggttacatt ttgtgtactg attctatttg cagacatttc ttatgcatta
    28381 aatatatgta taatgtattc tttaccaggt atagtaatag gaaagatgct aaagagaagc
    28441 tttgaacaag aggacagtaa taaattcgaa ccgatatatg gtggaaccat aatatttgta
    28501 ctttcaatgc ttgtatattt ttttgtatta aagacatttt taaatgtgaa tctattagat
    28561 gaagtttcta agatgatatc agaaattgta aatattcaaa aaaatagttt ttctgcaact
    28621 gaacttaaag tgtttgataa aatgaagcca gatgagattg taagttattt tacaaatatg
    28681 ctaccaatga tgttattttt acaagggttg ttatcagcgt ttgtcacata ttatttgagt
    28741 gtatttttta taaaaaggat tacaaagatg aatataagat ttcctaagtt tgctgatttt
    28801 tatttaccgg gaaatgctat actcacaact ttcttacttt atttattagt cttatttatt
    28861 caaattattg gttcaaaatt atatacagaa ttaataatga ctaatttaca gctagttttt
    28921 aatttaatgt ttgtaattca aggtatagca gtttgcattt atttcttaaa gaaatggata
    28981 agacagggac caaataaaat tatgtttttt tcagggataa ttttatgttt atttggattt
    29041 atgggaatat cttttgttgg tatggtagat agtataattg actttagaaa ggtaagaagc
    29101 tataaatcca cttaggagga tagaaatgag taataaacaa acctttaaat taaatatgcc
    29161 agaaataaat ttatatataa ttgttattgg tatttctagt ataattttgc tttattacaa
    29221 cttgtatgta gggtgtttat ttttctgtat ttttgtatat atggtttttc acaattggag
    29281 aactactaat attagacgtc atgaatggac agagtatatt caaaatttat ctttagatat
    29341 agatgaaaca actaaaaaag ccataataaa tcttccaata ccattatgta ttttagaatt
    29401 tgatggaaat atatcctggt ataatggaaa gttttatgat atgattggtc aaaaagattt
    29461 acttgataaa aatatagagg atatagttaa aaatcttaat ttaagaaaag tattaaatga
    29521 aaataaagag atgtacacag aaataaacta taaagaaaaa gaatatacaa taatttataa
    29581 tgttataaaa aatgatcaag aaaaaaatcc taaatatttg atgatattat actggataga
    29641 caagactgaa tatttaaaag taaaacaaaa ttatgatgat gaaaaaaatg caatgatgct
    29701 aatacaagta gatggatatg atgaagtatt aaaaagtgca gcagaagata agagagcttt
    29761 aattaatgtt gaagtagaga aaatactttc tgctttagaa ttaaattcaa atggtgcatt
    29821 gagaagaact tcaaaagaca aattcttctt ggtaatgcat aaaaaagagt taaaaaaatt
    29881 ggaagctgag aagttttcta ttttagatac aataagacat attgattatg gaaataatct
    29941 tcctgttact ataagtatag gaattggtat agatggagat acattaaatg agaacctaaa
    30001 acttgctacg ggtgctctcg atttagcact tggtagaggt ggtgaccagg ctgttgtaaa
    30061 aacaaaagat aaatttgtgt tttatggagg aaaatctaaa gctgtagaaa agaaaaccaa
    30121 agtaaaatcc agattaatag gtcatgcttt aagagaggtt atacaacaaa gtgaccaagt
    30181 ttatataatg ggacataaat atcctgatat ggatgctatg ggagcagcag ttggtgttta
    30241 tgatatatgt aaatcctgta acaaaactgc aaacatagtt ttacaatctg taaatgaatc
    30301 tatagaaata tttataaata aaataaatga aaataattac tataaaaagt tgtttattgg
    30361 aaaagaggaa gctattgaca actgcactaa aaatacatta gttgtagtag ttgatactca
    30421 tagaccaaat tatactgaat gtgaagaact gttaaagcta tcagagaagg tagtagttat
    30481 agaccatcat agaagaggtg tagagtttat aaatgatgca gtgcttttat tccatgagat
    30541 atatgtatcc tcaacgtgtg aaatggttac agagttagtt cagtatatgg atgaagatgt
    30601 aacaataaat aaacttacag ctgaagggct tttagcaggt ataagtctag atactaaaaa
    30661 ctttgcattc aaaacaggtg ttagaacttt tgaagctgct tcttacctta gaaaagttgg
    30721 agcagatact atagaggtaa agaaattctt caattctgat gtaaaagatt tcataataaa
    30781 agctgaaata attcaaagta ctaaaataat taataataga atatgtttag catattcaag
    30841 tactgagata gatagtataa atgttataat agctcaaact gctgatgagt tattaaatat
    30901 caaagaagta gaagcttcct ttgtattagg tgaaaaagat gatactatat ttataagtgc
    30961 gaggtcttta ggacaaataa atgtacatgt acttatggaa aaattaggag gcgggggaca
    31021 catagatata gctggagcac agttaaaaaa tgtttctcta aaagaagctt ataagatggt
    31081 aaataaaata atagaggagt atttggagga ggaagaatag atgaaagtta tattattaaa
    31141 agacgtaaaa ggaactggta aaaagggaga aatgaaagaa gtaagtgatg gatatgcaag
    31201 aaatttctta tttcctaaaa agatggctgt gcaagcagat agtgtagcaa taaaagaatt
    31261 aaatgaaaag aataaatcta aagaaataaa agcacaaaaa gagtatgaag aagcagtttt
    31321 attaggtaag caaatggaag aaattaatat agaaatatat tcaaaagcag gagaaggtgg
    31381 aagattattt ggttcaataa cttctaaaga aattgctgag caattaaaga aacaaaaaga
    31441 catagatgta gataaaagaa aaatattatt agatgaacca ataagatctt taggttcaac
    31501 tttcgtagaa ataaaaatac atcaaaaagt aactactaaa ataagagtgg atgtaaaaga
    31561 aaaacagtaa taactagagg tgattaagat ggaagatatg acgagaattc ctcctcatag
    31621 tgtagaatca gaacaatcaa tattgggttc tatacttcta gataaagatg ctataataac
    31681 ggttacagaa actataaagc ctgatgattt ctataaagag gctcacaaaa taatatatga
    31741 atgtatgata actttaagta ataaaggtga gcctatagac ttgataacat taacagaaga
    31801 actaagaaaa caagggcatc ttaatgatat tggaggaatc agttatatta caagtctttc
    31861 tactatagta ccgacgactt caaatgtaaa atactatgct gatatagtaa aagaaaagtc
    31921 tgtacttaga aagcttataa aagcttctaa tgaaataata aatctaggat atagtggagc
    31981 aactaagatt gaagatgttt tagaacaagc tgaaaagagt atatttgata tatcacagga
    32041 gaaaactagt gatgatttta aatctataaa cttagttttg atggatgcct atgatatgat
    32101 agaaaaatta tacactaaca agagtgatgt cactggtata actactggtt ttaaagattt
    32161 aaacaaaaaa ataaatggtc ttcaaaggac tgatttaata ttaattgcag ctagaccagc
    32221 aatgggtaaa actgcctttt ctttaaactt ggttcaaaat gcagccttaa aaggtgatgc
    32281 ctctgtagct gtatttagtt tagagatgtc taaggaacaa ttagtacaac gtatgttatc
    32341 ttcacaatct agtgtagaat taaaaaaaat aaagactgga acacttaatg ataatgattg
    32401 gcctagaatt atagatgcta tggcagtatt atcagatgcc aaaatacaca tagatgatac
    32461 tcctggtata aaaatatcag aactaaggtc aaagtgtaga aagttaaaaa tagaaaaagg
    32521 actagattta gtgcttattg attatcttca acttatggaa ggtgaaggta ataatgaaag
    32581 cagacaacaa gagatatcta agatatcaag gtcactaaaa atattagcaa aagaattaaa
    32641 ctgtcctgta gtagcactat ctcagttatc acgtgcacca gaacaaagag cagaccatag
    32701 acctatgtta tctgatttaa gagagtctgg agcaatagaa caggatgcag atatagttat
    32761 gttcttatac agggacgaat attatcatgc tgattctgag agtaaaaata taggagaagt
    32821 aataatagca aaaaatagac acggtgaaac tggttctgtg gaacttgttt ggcttggtga
    32881 agttcaaaga tttggggata agttgagaga cctataatct aacgaatatc cacagaacgt
    32941 cctttctttc tacaggaaaa atgtggatac atgtcactaa atatgtccaa acattgaaat
    33001 ttagctaaaa aactatccac actatccaca gatggcctgt tgataagtgt gaataagtag
    33061 ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta ggaggaataa
    33121 tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac ttgatgaaat
    33181 aaacttattt agagtaattg acaataattt aagagaatct attgttgttt atgctaataa
    33241 tgtggataat cagtatcata taaacatgac taatacaaac tttggaaata taattaacat
    33301 atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta taaaatatga
    33361 aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt taaatattgg
    33421 agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa gtaaagtgtt
    33481 cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg ttggttctca
    33541 atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag cagatgtagt
    33601 agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag gtaaaaaata
    33661 cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata taatcggaaa
    33721 tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta aaacggataa
    33781 tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc catatcataa
    33841 agaactagat gcattgtcag aagaagccag aggcgattta aagataggta caacaaaaaa
    33901 aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa tatgtgactt
    33961 aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta aaaatgaaat
    34021 agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat ataacgaata
    34081 tctaggatat gcagagcaaa taagaaaata tgtggcagat acgtctgtta ttgtatatga
    34141 tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt tattagattt
    34201 agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg gatttgcaat
    34261 tggtgcaggt atagggccaa atatgataaa agacgttgtt ggtatagtaa aagcatatac
    34321 tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg gagataagat
    34381 aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat gtgggtggtt
    34441 tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta tttcatttat
    34501 gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt ataaaatggg
    34561 cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat gtgaacctgt
    34621 atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact ttgatgacct
    34681 tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag taagtgtaga
    34741 tatggtttct gttggtccaa atagagctca aactataata agaagaaata tatttgctta
    34801 atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag ataatgttta
    34861 tagttattag agatgctctt ttatatttga cttacattga ttttttatta agttaaagct
    34921 acttatacat attctaactg aaattaaagt tgaatttatg tagatacata taggtttttt
    34981 aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt ctaaaaaaag
    35041 taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt gagtgaaaga
    35101 aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag cacctgactt
    35161 ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg ctcgttggtc
    35221 aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc ctacgagtca
    35281 ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg
    35341 ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga ttatatgctg
    35401 gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt cgattcctat
    35461 caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt acaggatact
    35521 tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta aacatacaac
    35581 aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagc
    35641 ataatattat gtaatatagc aaatttaatt tatagtatca ataaatatag actactaaga
    35701 atgtaaatga tttgttagat taaggtacat tagatatgta aatagaaaaa taaaaagtat
    35761 gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt acctctctat
    35821 ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc atctcaaatc
    35881 atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag ttctttgagt
    35941 gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt ttttaccagc
    36001 aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc
    36061 agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc
    36121 acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct
    36181 aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt
    36241 gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt cactattatc
    36301 cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct
    36361 aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt taaaattcga
    36421 aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat
    36481 ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga
    36541 aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct
    36601 ttctttcttt acttttaaag attctatatt ctctttagtc ttctttacaa tttcatcttt
    36661 agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag aaatttcatc
    36721 atctatagat tttatctcag ggattctatt atatacctca ttttttctat gttctaataa
    36781 gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc
    36841 attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc
    36901 tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt ttttttgtaa
    36961 gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt
    37021 tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta
    37081 attataccat ttatataaga tatacttggg tttgaaatat tcttagactt agaacatgca
    37141 tttattatca aatctatatc catattaaat ttatcaaacc aggtgtccat aagttctttt
    37201 tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata
    37261 tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata taaatttgaa
    37321 tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt accagtttta
    37381 tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt
    37441 atatccataa tactcatctt ttcacttggg tcgagatatc gcccaactat tttatttata
    37501 gaattaaaca tttttctaat acttggattt tcagaagtag atacaacttt atctgtatta
    37561 gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa
    37621 aattctattg aataattaaa attatcatat tcttcattgt catgtatttt tataatcttc
    37681 ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt taggtttttg
    37741 gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct
    37801 aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc tataaagata
    37861 ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca
    37921 cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca
    37981 caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg
    38041 gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta
    38101 ttaataatag gttgctttat agaaaaggat gacagtttag aaacatacaa agaaaaaact
    38161 aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac taagaggtta
    38221 atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacataga taaaatatat
    38281 ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa
    38341 atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta
    38401 aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata
    38461 gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta
    38521 aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa tgttactaat
    38581 tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact
    38641 attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat
    38701 aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg
    38761 ctatatgaaa tagaagctga aaacgttaga gattttgcat ttatactaag tagtaaattt
    38821 gatgtaaaca aagtagattc aaagggagta gctatcaata catataattt aaataaaaag
    38881 ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt
    38941 ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg
    39001 gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta
    39061 ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat
    39121 gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat
    39181 tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag
    39241 acaaaaagtt atttgagtga aaatatattt aagccagcaa atgagtataa aaattctact
    39301 gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa
    39361 gttggagata aagtattctt tgagacttta aatgagtact ataataaata taagcataaa
    39421 aatgctaatg gaagagcttt tgtggaacta tggaatagta aaggtgtaga tataaataag
    39481 attataagtg aatgtaagta aatagtgaat gtaagtaaat tttgtgcaga gtattaaatt
    39541 ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa agaggtgaag
    39601 gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac tatatagggt
    39661 ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata actacaggtc
    39721 ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca catgagcatg
    39781 ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca gtatttgcta
    39841 atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat aaaaatatga
    39901 aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga cctttttcaa
    39961 tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt gatgaaaaaa
    40021 tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat ctatataaat
    40081 ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg ggttcatata
    40141 catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat gaagatgcag
    40201 ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta gcacatttaa
    40261 gcaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta ttagcatcaa
    40321 atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat gatgtttcta
    40381 atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt gaaaatgttg
    40441 aggataataa aattttatac tctataaagc agggtatgat tttagcgata cctgctatta
    40501 tgactggttc aactgctctt gtaattttga atcttccaat aaaggcttat caagaatatt
    40561 tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat gattctactt
    40621 taggaattat ttcattaata atattattaa caataagcta tagttatgga aaaatatatg
    40681 ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta gtattttcac
    40741 atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta tttacatcta
    40801 taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct tttgttacaa
    40861 gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt tcggctatag
    40921 ttcctttcat aatagtagta tttttgtttt ctatttttag agtggtaatg ataagtctaa
    40981 ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt tttaataaaa
    41041 tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt atgtggtttt
    41101 ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt gaaaatagta
    41161 tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt tttactaaga
    41221 ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt ttagtaattg
    41281 caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt gcttcaatac
    41341 cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt tttaactata
    41401 ttatgtttgt accttttgta attactccta taatcttaac cgttacaagt tatgtggcta
    41461 tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca cctatatttt
    41521 taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta tttaatttat
    41581 gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga tatacatata
    41641 tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa ttaacaggtg
    41701 aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa atgttggcat
    41761 cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa ccacaagtca
    41821 attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat tcaataggag
    41881 ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc ttagatgagc
    41941 ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa aagctattga
    42001 gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac cctaagttac
    42061 cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg ttaggtatag
    42121 aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt atcaatgcga
    42181 tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat agctctctta
    42241 tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta gttaaagaga
    42301 tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt tcaaaaaatt
    42361 tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat aagttatatg
    42421 gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct tttgaagagt
    42481 ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg taaattctag
    42541 taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat aagtatagta
    42601 gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt taagaaaagg
    42661 ctttctaagt attgtaagtt agaaattata gagttggatg atgaaaaagc tccagagaat
    42721 ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat tttagggaag
    42781 ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt atcctctgaa
    42841 gagcttgcta agactatgag tgatttagca gttagaggta atagtagtct ctgttttata
    42901 ataggaggtt ctttagggct ttctgatgag gttttaggaa gggctgatta taggttatcc
    42961 ttttctaaga tgacttttcc tcatcagatg atgagattga tattgttaga acagatttat
    43021 agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg gtttttgtta
    43081 atttgacact taataatatt aaaataacaa aaaagtattg aaagtatatt gcatttaagg
    43141 tagaattaaa taaaatatta taacattata atgtttatat ggttgttaat aaaaatttgg
    43201 aggaaaatat atgataaaaa taatgttggc ctgttcagca ggaatgtcta caagtttatt
    43261 agttactaaa atggaaagtg cagctaaaga aaatggtata gagtcgcaaa tttgggcaat
    43321 accagaatca actatacaaa atgaaataga aaaatgtgat gtattattac taggaccaca
    43381 agttagatat gtattaccta aggctcaaga aattgctaag ccatataata taccagtaga
    43441 agttataaat atgatgcact atggaacagt taatggagaa gctgttttaa atagagcgat
    43501 agaacttaac aacactaagt aatataaatt ataatgtatt tttaatatat aaattgggag
    43561 aatcaaagga tggatgaaaa gactatagaa atatctttta atataatagg ttatgcaggt
    43621 gaaggtaaag gattagcttt tgaagcaata aaagaggcta agaatggaaa tatagaaaaa
    43681 gcaaaagaat tattaaaaga gtccagggaa gttataaata aagcacatag atatcaaaca
    43741 gaattaatac aaaacgaagc tagtggaaac aggactgaaa taagtgtcat acttatacat
    43801 gcacaggacc acttaatgaa tggcatgaat tttcaacagt tagctgagga aatcgttgat
    43861 ttacatttga aattacaaga taaataaaaa ttaaagataa taaattaaga gagttcttaa
    43921 aagatacatt atgtgtagca cactaattgt tagattattg attcaatatt tagtgagtaa
    43981 tacattagga ttattttgag gactcttttt atatttagtt taaagttaat aaatatttat
    44041 tattaataat ataagattat atgttataat attataacat ataatttaag aggtgaatca
    44101 tgttaagaaa tcaaccatta tatatacaag ttgtagatat attaaagcaa aaaatattaa
    44161 ataacgaata taaaattgat gaaaatattc caacagaaag ggagttagag caagagtttg
    44221 gagtcagtaa aattactatt agaaaagcta ttgagatatt agaatcagaa gggtatgtag
    44281 agaaaaaaag tggaaaaggg acaaaagtaa taagtaatag tctttttaat aagctatcaa
    44341 aagcagagtc tttttcaagt atacttttat ctaaaggtca taagttatct aaaaagacta
    44401 tttcaattga aaaaataata aatgatacaa attcacaagt atttagtata tttggagaaa
    44461 aatgtagcaa gattactaga atgtattact tagatagtaa gccatatata ttttttactc
    44521 attatattcc ttttgatgaa aatctaactg ttgaaagttt taataatgaa agttctttat
    44581 atatgtactt atataggaga aatataaata taactagatt tgaagatgaa ttttttatag
    44641 aagaatcaga actagaggta tcaaaggctc taaatctagc taaagacata ttattaggaa
    44701 gaaaaagaaa gacctatgat gagaatgata atatagttga gctatcattt gcaagatata
    44761 atacagaagt acaaaactat gtaattaaat acgaaatata ataagaatcc tgagtttatt
    44821 gaaatgataa tatgctcagg atttttgttt agttgtaaaa ataaaaaata ttataacatt
    44881 ataatgttta caactaaaca ttataatgtt ataatatttt taataaacaa gtgatattat
    44941 caattacata aaaattagtt tatgttatga aatcgttatt gcattataaa taaaggataa
    45001 tatcaataat acagggggat aactatggaa aaattcgcaa gttttttaga gcgcaagatt
    45061 atgcctgtag cagcgaagat aagtaatcag agacatatga gagctgtaag agcaggtata
    45121 atagcaacat taccactaac aatagtgggg tcatttttca caatattatt aaacatacct
    45181 attgatgctt atatggattt tatagctcca tttaaagcaa tattagatat accatttaga
    45241 tatactgtag gatttttatc tctatatgct acttttggga tagcatcatc acttgctaaa
    45301 agctacgatt tagatacaac aggtgttggt atgcttgcag ttatggcatt tttagtttct
    45361 acaatagttc caactcaagt agttgaacca gtaggtggag ttatagaagc tggtagatgg
    45421 atggatatgg caaaacttaa ttcgccatca ttatttggag ctatcgtaac atcattaatt
    45481 acagttgaaa tatatcgttt tatgaaggaa aagaatatta caattaagat gccagcagga
    45541 gttccagtag aggtttcaaa ttcatttact gcattattac cgacagcagt aattttaatg
    45601 ttattctggg taataagaca tattttagga tttgatataa gtgttgcact aagcgctatg
    45661 ttatcacctt taaaaggatt cttatcagga aatagtttag gtggtgggtt actgacagta
    45721 ttcctaataa ctttattctg ggtacttgga atacatggtc cagctataat ggggccagta
    45781 ataagaccta tatgggacat gtctatagca gaaaatataa ctgcattcac agaaggagta
    45841 tcagctcatg caatgccaaa catatttaca gaacaatttt tacagtggta tgtatggata
    45901 gggggagcag gggctactct tgcattagtt gtaatgttta tgttttctaa gtcacaatat
    45961 ctaaaaagtt taggtagatt aggattttta ccagggttat tcaacataaa tgaacctata
    46021 atatttggag caccaatagt tatgaatcca atactaggga taccatttat aattggacct
    46081 ataataatga caattctatc atatgttctt actattacag gggttatacc tatgatggta
    46141 gcaagattac catttgcaat gccagcacca atagcagcag taatgagtac taactggagt
    46201 atattagcag gtatcttagt attaatcaac tttgtgatat catttgctgt ttattatcca
    46261 ttcttcaaag tttttgaaaa acaacaatta caaagagaac aagaagaaat gagtgaaaat
    46321 ttataaaaat acatagatat agaggttttg tactatataa aatatagaaa aaacttataa
    46381 gttattaagg gtgaaggtag gaggaatgac ctttgccttt aattttatat attttaaata
    46441 atgtaatttg aggaggagaa agatggaaag aagattagga atatcagtat atccagagca
    46501 tagtaacatg gaagatgata aggcatatat aaaaatggct tcaaaatacg gatttaaaag
    46561 aatatttatg tgcatgcttt caattacaag acctaaggaa gaagttaaaa aacattttaa
    46621 agagattata acctatgcaa gagaattagg atttgaagta atccttgata tagctccaaa
    46681 catatttgat atattacaaa tatcttatga tgatttaaca ttttttagtg agttaaatgc
    46741 tagtggaata aggcttgact atggatatga tggaagtaaa gaagctatga tttctttcaa
    46801 tccatatgat ttaaaagtgg aactaaatat gagtaatgat gtagcatact tggataatat
    46861 aattacttac aagccaaatg taaataatat atatggttgt cacaattttt atcctcaaag
    46921 gggtacaggc ttgccatatg atttttttgt aagatgttca aatagattta aaaagtatgg
    46981 aataagaact gctgcttttg ttaattcaaa atatgcaaca atcggacctt ggaatataaa
    47041 tgatggactg tgtacgcttg aaatgcatag aaacttagat attgctacag caactaagca
    47101 cttattcgca acaggattga ttgacgatgt aattgtaggg aatgcatatg caagtgaaga
    47161 tgaattaaaa agaatgtctg aagtaaataa atatcaagtt gtatttaatg tagtaactga
    47221 ggaaaaaact tctgaagtag aaaagacaat aatggcaaat gagcaacact ttagaagagg
    47281 ggatataacc aaccaagtag caaggtctac agaagtaaga aaaaaatatg ctaaagaaaa
    47341 tttccctaag catgataata cgaccatctt taaaagagga gatgtagtga ttggtaatga
    47401 tgactttgga aagtataaag gggaattaca gttgatactt caagattgtg cagatgaaag
    47461 aaaaaataag gtaggtagaa taaaggaaga cgaaataatg cttttagact ttattgaacc
    47521 ttggagtaaa tttaaactta ttttagaggg agagtaaagt gaagatagac atattaataa
    47581 aaggtggaaa aacagtagat aaaagtataa ttgatatagc tatattagac gataaaataa
    47641 tagaagtaaa aaatcagata gatgattcta agtatcaggc aaatcaaatt ataaacctag
    47701 atggagaaaa atttattagt gctggttgga tagatataca tacgcattgc tacgaaagac
    47761 tagaccttta taaggattat ccagatgagg ttggaataaa aagcggtgtt actactgtag
    47821 tagatgcagg aacaacagga gctttagata taggtaaatt ttatgaagat actaaaaaat
    47881 ataaaacaaa tgtatatgct ttaataaata ttgctaagca gggaataaca tctcaagatg
    47941 aactttcaag tatgatgaat attgatgaat atgaacttaa aagagcagtt aaaaaataca
    48001 aagattttgt tgttggaatc aaagctagaa tgagtaaaag tgttgtaatt tcaaatgatg
    48061 ttgaaccttt aaaagtagct aaaagaataa aaaatgaact taatctacct atgatggttc
    48121 actttgggag ttctcctcca acgatagagg atatatttga ctatatggaa aaaggagata
    48181 tattaaccca tatatataat ggaaagccaa atggaatact tagaggcaat gaggttaaaa
    48241 aagaaatcat agaagctaga gaaagaggta taatccttga tgtaggtcat ggaaccgaaa
    48301 gttttagtat ggatattgca atgaaatcta aggatgcagg aatttttcca gacactataa
    48361 gtacagatat atatattaaa aatagaataa atggtccagt gtataatcta tctacaacaa
    48421 tggaaaagtt tatatatatg ggctattcac ttgaagatat aatagataaa gttactaaaa
    48481 atgcagcaga tgcaataagt ttaaaaaata aaggtttaat aaaagaagga tatgatgctg
    48541 atttaactat atttgatgtg gttaatgaag aaaaagaact tcaagattct ttgaataagt
    48601 cagtaatcac aagtacgtct ataaagccaa gagcagtagt agtcaatgga gaatatttaa
    48661 atataggtaa gtctatagga gagaatgagt agaagggaga aactattgta tgagtagtat
    48721 atttgaaaaa tactctgtaa gacgtgttat aaatgcttca ggcaaaatga ctatactagg
    48781 agtatcaaca gtagataagg aagttattga agcaataggt gaagggtgta aaaatttctt
    48841 tataatagaa gaacttataa ataaaacagg tgaatatata gcaaatttat tagggtgtga
    48901 aagtgcacta atagtttcta gtgcatctgc tgggattgca cagagtgtgg gagcaatgat
    48961 agcaaaagat gatatgagtc tagtttacaa tgtaaataat cctaagaaac aagttaaaag
    49021 ggaaataata attccaaagg ggcataatgt tgattatgga gttccagtag aagtaatgat
    49081 aagacttgga ggaggagaag ttgttgaggc cggctatgct aatatgtgta acaaggaaca
    49141 tataataaga gagataaatg aaaatacagc agcaattatg tatattaaat ctcatcattg
    49201 tgttcaaaag agtatgctaa gtgtattaga tgcagtacaa gttgctaaag aatataattt
    49261 acctttgata gtagatgcag cagcagaaga agatttaaat gtatattatg aattgggagc
    49321 agacctagtg atatacagtg gtgctaaggc tatagagggt cctagctcag ggttagtaat
    49381 aggtaagaaa aaatacatag ataatgtaaa gcttcaatca aagggtatag gaagagctat
    49441 gaaaatagga aaagaaaata ttgtaggtct aacatgtgca attgagagat atataagtaa
    49501 tgataaagta actttaaaag aaatggagga caagctaaat ccatttatag acaagataaa
    49561 tactataaag ggagtgagtg ctagtataac tagagattca gcaggtagag aaatacttcg
    49621 tggagaaatt gactttaatg aagatataat aaataagtca acacaacaaa ttataaatga
    49681 acttagaagt ggagagatag ctatatacac tagagactat aaggctaatg aaggcaagat
    49741 tgagattgat ataagaagtg taactggaag tgatttagat actatttata ataggataaa
    49801 aaatatagta gaaagttaga gtgtagagta agtgacatta taatgattta ataaattaaa
    49861 ttttaccaat ggaggggata aatatgaatt gtataccaca atattataaa aatagagttt
    49921 gtttaaatgt tcttgcagga agcataaaaa atgctaaaga agtatatgat gcttgtgatg
    49981 ggtatgtatt gataggagta ctatcaaaga attacagtac tgtagaagca gctattgaag
    50041 atatgaaaaa atactcaaag gaaatagata atgcaatatc tgtggggctt ggagctggag
    50101 acccaaatca gtcaaatatg gtatctcaga tttctaaaga acttcagcca caacatataa
    50161 atcaagtgtt tacaggtgtg gctacgtcta gagcattgct tggtcaaaat gatagtataa
    50221 taaatggatt agtttctcca actggtaaac caggatttgt aaaaatatcc acaggtccat
    50281 taagttcaaa agaaaaagat ggtatagttc ctatagaaac agcaatagct atgatgaaag
    50341 atatgggagg aagctcaatt aagttttttc caatgggagg gcttaaaaca aaggatgaat
    50401 atatagaagt tgcaaaagct tgtgcaaagc ataattttta tttagaacca acagggggta
    50461 tagacttaga taacttcaaa gagatagttc aaattgcact tgatgctggt gtagaaaaag
    50521 ttatacctca tgtttataca tcaataattg ataaagaaac aggtgaaacg aaagtggaag
    50581 atataggagt actttataaa ataatgaaag aattattggg atagatttat gaatatatta
    50641 gcttttggtg aaataatgat gagactttca gtacctgact acaagttctt aacacaaacc
    50701 aatgaactaa attatataat tacaggcaca ggattaaata tattatctgg acttaaaaac
    50761 tttggataca atacctatat gttgacaaag ttgcctaata ataatgtagg taaagcatca
    50821 agtgcaaata taagaaagct tggagtaaaa gatgacttta tcacatatgg agggaatcat
    50881 ataggagtgt attttcttga aaatgggtat ggagaaagac cttctgaagt gacttatttg
    50941 aataggttaa atagttcatt ttgtgagagt aaattacaag attatgatat tgataaatgt
    51001 ttagaagaaa tggatatagt ccttatttgt ggtattgcta tgcaacttac agattcaatt
    51061 aaagagattg ctttagaact agctagaaaa tcttatgaaa aggggataaa agtattcttt
    51121 gattttaatt ttagacaatc attaaatcca gaaaggactt atgatgattt agtaaatgat
    51181 tataagaaaa tactaccata ttgtcatggt ctatttgcta gttatagaga tataaataag
    51241 atacttaata tagatagtct aaatataaaa tctagttcag aattgattaa aaaagagtat
    51301 gatataggta ttgtagctgg aactatgaga agatttgatg atatgaataa tagatttata
    51361 aagggatata tatcaaatga tgatggttat tttgaaagta gggagtataa gttagaaata
    51421 tatgacaggg ttggtgccgg agatggatat gtagcaggaa ttatatattc atatttggaa
    51481 aatatgaaga gtgaagatat agttgagttt ggggttgcta gttcggtgtt agctcataca
    51541 acttatgggg ataattcttt agtaagcaca ggtcatgtac ttagacttat gaacaatgaa
    51601 aaaatagata tattgagata atacatttgt atataataaa atttttatct tttttagaca
    51661 cacttttgac ttgtttatat ttgttaaaca gagttatttt taatgattat aaatattaaa
    51721 ttaaaagtta aataataaaa tagtagaaaa tagcattaac tatagaaaaa atggttaatg
    51781 ctatttttat cgttaatatt gattaatttt atattataga aatgaacttt tctaatgctg
    51841 gatttgtatt ttttttattc catacaacta caacttcaat tctattttct acttctttta
    51901 taggtactat tgcaatatca cgttctatca ttgtatctgt aacacaaaat gaaggtaaaa
    51961 tggatattcc catgttagaa gataccaatg attcaatagt attaagatta tttgaacgtt
    52021 gtaccacatt aggtacaaac cctgcttcac gatatctatt ctgtatcatt gacttttgtc
    52081 catactcatc ccctgtttct tttacaaaaa taaatttatc atgttgtaat tcaattaaat
    52141 cgatagaatc ctcattgtaa agtggatgac caggaggtaa aagagctacc aaagaatatc
    52201 tttctacagt tttatattca aaatctttta atggttgatt ttctggatta ataacaaaag
    52261 cgaggtccat agaattattt tttactaagt ttaaaagttc ggttatttca ttagtacaaa
    52321 tatccataga tatattaggg tatgtattag aaaattcttt taaatattgt tgaagtttgt
    52381 ttttttcata tcctattaag gttccaatgc tcaaagttcc aaaaaaacca tgtgcaaatt
    52441 gttgtgtttt tgcaatagca tcattgatat tttttattat agtacgtgcc tcagataaaa
    52501 aaacttttcc agctggtgtt agttcaacat gacgtttact tcttgtaaat agagttacat
    52561 ttatttgttc ttctaaagct tttatttgct gtgtaacagc agtttgagaa atataaaatt
    52621 tttctgctgt tttggtaaaa cttaaatttt ctgctacaga tataaaatat tctaattgtt
    52681 tagtattcaa ataatcaccc ctatttaaac ttgattgttt gtaaacctta tttatctata
    52741 attaaagctt attatagtat aagaaaattt acttttaaag aatattagaa aatatttgta
    52801 taaaaatata tgttttaagt atatctttac attagatttt ataaatttat taataagaat
    52861 aacttatata ttccaatgaa aacgatattg gatttaagta agtttgtctc ataaaatgaa
    52921 agtatatcaa ttgataatac tttaaataag aagggagaaa aaatggaaga taaattttat
    52981 gcaaaaggca acggaaataa cggatatatt aaaaatcttg aagtttgttc ctttaataac
    53041 ttagatggaa cttgtggaat gtttcaaatg gctctgtaca aaagagatga aaaatactat
    53101 ttatatggat gctgttttgg aggaaataaa aaaaatggag taatgattag cgatattaca
    53161 gacccttata atccacaatt tataaaacat tttcaaatgt tagaccctaa agagtatcct
    53221 acaacaacaa ctcccaaaat tcaaatagca gatgatttaa tgatagtagc aatgagttgt
    53281 ggaagtggac caggagcact tgttgaccaa gctaaattag caaatattaa gtgtgaagca
    53341 ggaattagaa tatatagttt aaaagaagac cctttaaatc ctaagttttt aggatattgg
    53401 gattgtggct taaagcatgt aatgggtgtt catagattca tgtacaatgg tggaagatat
    53461 gtacatttat caagtgattg tgttggcttt gaaggtctga tttatagggt catagatata
    53521 ataaatccta ctaatccagt ggaaataggt aaatggtgga gaccagacca atatgcagat
    53581 ggatatccaa atagaacttt tgatgcagga gcacctcatt gcccagaatt tatggataaa
    53641 ggatggcttc atggacctcc atttgtaaga gacggaaaag catattgtgg ttatggagga
    53701 gctggtttag ttgtattaga tgttgaagat ttaacaagac caagatgctt aggtgaattg
    53761 ccatttatgc ctgcattttc tagtagactt gcaggtgcaa gaactcatac agcattacca
    53821 ttgccaggaa gagatttagt cgttgttcaa aatgagggag aaagattcca gttctttaaa
    53881 ccagataaca ttacagatgt tcaagctatg aataatatac atatggttga tgttagtgac
    53941 ccaacaaaac caacattaat tgctcaattt ccatatcctg aagttccaaa agatttccct
    54001 tatcctaact ttaatgttgc aggattagga aaaccagggc catttggtcc acataatctt
    54061 catgaaccaa tggataataa accatggtta gagcaaagag gagatagagt atattgctgt
    54121 tatttccatg cagggctaag ggtttatgat gtatcagacc catattatat caaagagcta
    54181 gcatatttta taccaccaaa tccaaataaa acaccagaag aatcttattt cccaggattc
    54241 ccaggaccac gcttggcagt aacagaagat cttatcgttg atgatagagg ctacatcatc
    54301 atagatgctt tagatgatgg attctatata ttaaaaatga aagaagatta aataaatact
    54361 aaagagggag agttgaaaat gaaaatacta ggtatttctt ttggaacaaa aaatggaaat
    54421 aatgacacaa tgtgtaaagt tgcacttaaa ggtgccaaag aagcaggggc tgaagtagaa
    54481 tttattcaaa tgtcatctat taacattaaa cattgtactg gatgttgtgc gtgtgttaaa
    54541 actttattat ctgggaaagg tagtatgtgt gtattaaaag atgattttga atggttgctt
    54601 gacaaaatga aagatgcaga tggtatagta gtttcagacc ctatttttga agaaggggca
    54661 tcaggattat tccatactat aatggaccgt tttggaccca gagcagatag aggaaataac
    54721 attataggaa caaaagttgc agaagctata ggaggaaaaa ttccagattc aagaatgctt
    54781 aatgataaag taatatcatt tatgggaata ggaggttctg actggggtac aagagttcaa
    54841 tgtgaacatg ctatgctagc tctcatacca atgtggaaag ttatagataa tgcttggttc
    54901 ccatgggcaa aggaattagt aatggatgat acaaggcttg ttcaagttca tcaaattggt
    54961 ttaaatatag ttgaagcagc aaaagatttt gaaaaagctt catatcaagg agaagaaggt
    55021 gtatgtccac attgccacaa tagattattc taccttgagc caggtactaa aaaggcaatt
    55081 tgtgctctat gtggtatagt tggagagtta gatactataa gtggaaaaac tatatttagt
    55141 tttccagaag agcaattagg tcatgctcat gatactttac caggtaagtt tatacatggt
    55201 gatgatatta agaaaatgga aggtcattat gcagaagttc gtaaaacaca agaatttaaa
    55261 gaaagaaaaa catcttataa ttttatagag acattaacta aagaaaaaca agctggagtt
    55321 tcattaggat aataattaaa atactaagac tcttttttaa gggatgttag atgttatagg
    55381 aggcttattg tatgaatgaa aagccaagtt ttaaacaaat tgaagttatt ttagcagtta
    55441 tttttttggt acttacatat tacaaggtta gattaactcc agcatttatt gttatattta
    55501 taccactata tttaagttat tttgcagtat ctatgattgt aaatcaaata gcaattaagc
    55561 tattggagat aaggcaagct agggaagcta ctaattacaa tataggaaat ggatagaaag
    55621 aaaaaagaaa tattaacact gtctatgggg attgctttac tacctccttt atgggcagta
    55681 ttagcacctt atataggaat aaaaactgga gctgtagcac taatctgtgc aggattatat
    55741 gtaactaatg gtaataaaca agaagatgga ttaaaaataa tgtttggttt ttggtgtgga
    55801 gatttatggg cagtgttagc cattttaatt atgggatata tgaatttcaa tcaaaaccta
    55861 gaattatttt taacacttag tatattaggt ttttttgcag ttgtaattgc atccttattt
    55921 gaaaaaataa ttttcctacc atcatggctt tgtggttggg ctataggtct aactattatg
    55981 acaggtgaaa atattattaa tttacaagat atttgtattc aaattgctgt agctatggca
    56041 gtaggagtat ggtacgtggg tgcaggtgta gatttgttta caatatatat tttgagtaaa
    56101 gataggaaaa agggaaattg aaagcatgtg aatatacttt gattatagga ggatggctat
    56161 gggttttgaa tttaaaataa tgagaagttt aatatatgta ggacttgcca aggaagaata
    56221 tagacctaag ctaatggact ggttatatcg tcaccatatt ccagatagta ttagcacttt
    56281 tggaccatat tgtactaaat atgcctttta tcaagcatat cctacaccaa atgaaggtga
    56341 gcgttttggt gcacgtaaga tgcaactaac agaacattat tggcttgtag atgaacatat
    56401 gcctgagatg gcaaatagaa ttatgacaga atatatgcct atggatgttc tacgttggca
    56461 agggtgtata ccagatgtag aaaataaaag ggttcatgaa aatgcagaaa gtggagatgc
    56521 aggacgtgca gtaggtggag ataatggatg tccaccattt atatttgcct ttgttccaat
    56581 aaactgggaa gaagacttta gaggaaaagg acgtactgta caagatggac caaactatcg
    56641 ttggcaattt atgattaagt atccagatgg tatctctaaa gaagaaggag aaaaatggtt
    56701 ctatgatgag gtagtgccat actttacaaa ctgttgctat gttaatcgtt ttgtcagtag
    56761 taaaataatg attaattatg gagcaactgc ttttgaccgt gtgtcagaac tatggtttga
    56821 aggggaagaa gaatggtata aagctgtggt tgaagagaca aagtcgttta ttaaaaaacc
    56881 agaatgggca caagaagagg agttcccata tttaaaacca caattcaata tcgcatcagt
    56941 attcttaggt gatatagcaa ctatggatgc atactcacag tatcgtggat atataccaat
    57001 gagataaaaa ttatagtttg tgataagtag agaaggcatt tactcttact ggtagaagaa
    57061 gacttattgt atccttaaaa gtaggtgaat atatagtttt atatttaaat ttatataaaa
    57121 ctaagactaa taaatatgaa acaatagaat tagaataatt tgataatatt tgttaaaaga
    57181 gcatttagaa tgtgtttaaa gattaagata ttccttttat ggtagcagat agttgaaata
    57241 ataaaactgt tatcatgaaa ggaatatttt tatgtatagg aaatttttaa aaataaaagt
    57301 taaatatgtt taagaacatc ttaaagtgaa agaatcattg ttagaaatat gtagacaata
    57361 tagtatttat aacgtatcat attaacaagt ttacacttgg attatgaaat aaaaagagta
    57421 attctaaagg ttgtaaatac tatgctgact ttgatacttc aaagtttagg tgattttgta
    57481 attgttaata gaaatacgtt taatgagcat aaagaattta tctaataata ggagaatata
    57541 atgtatacaa ttggacaagt atctaaattt ttaggaatat ctagagatac actaaaattt
    57601 tatgaagaca aaggattagt aaatcctaag aaaaatgatg aaaatggata tagaatatat
    57661 aatcaggttg atatctatga tattgcaaca ataaactttt atagagaaat tgatatagaa
    57721 attaaaaaga ttcaagaaat aagaaaaagt aaaagtataa acaatttaga actcttacta
    57781 gaagagaagg aacaaatcat actaaaagag atagaatata aaaagcttct cttaaaaaag
    57841 gttcagttag tcaaagaaga ttgtgacaaa atcaaaacat attttggaaa atacacaata
    57901 aaagaaatga agcctttgga agtaaaaggt aaaataaaac atgttactaa ctatgaggaa
    57961 tatgaagcct taaaagagaa tacaaacaac ttaaaaaaag cagttacatt aactgattta
    58021 agaagagtaa taaattttaa tgaagaaggt ataatagaag aaaaatttat aattgtaagg
    58081 gaagtagatg actacaacaa aatcatagaa ggtgaagttc tatctcatcc caattgtatt
    58141 tatactatta ttgaagatgg cagatggtca actggaggag aaaatattga taataaagtg
    58201 gaagacagat taagagaaat agcaatagaa aatgaatgtg aattacttgg aatagtctat
    58261 gttaatatat tgcttactac ttatgaagaa gggattgagc gtatattttt agagatttat
    58321 gcacctataa aataatataa aagcattgac ctgggactta ctcataggtt taaactcatg
    58381 atataagcag ttataactta tatagcaatt aaatattaat ttgaaatgag gattacttat
    58441 gagaacagag aaatcattaa aaaggcctat agccagtttt attattctaa caaatataat
    58501 ttttttacca ctttttttat tggtagtagt gactataatg ttaaaacttc caacagtgat
    58561 atctgacatt gcgttgtgta tatcagcgtg gtcatccact tttgcattta tgatattatt
    58621 taaaaaaatt tatccaggta agaaattttt agtacatgta aaagatagat ttagaaataa
    58681 acttaaattt tcaacagtat ctataataat tagtatacaa gtcctaatag ctgtagtagt
    58741 tatatttata ttagcatcta aaaatcatgg aataatgcct aactttacag tttcatcatg
    58801 gggagcattt atatatttat ttttaaaaaa tttatttgca ggatcattag gtgaggaatt
    58861 aggttggaga ggatttgttc aaaatcatct tcagaaaaga cattctcctt taaaagcatc
    58921 cataacagta ggtttttggt gggggatgtg gcatttacca atatggttta caacaggatt
    58981 caaaggactt gatttaataa aatatattat attttttatg attgcaatta tatcaatcag
    59041 tattataatg acaacttttt ataatctaaa taaaactttg ataataccta ttgcaataca
    59101 tcaactattt aattttttaa tagggataat taatgaagat ttaatagagc ttataaatta
    59161 ctatgcaatg ttatatttaa tatttgcaat tgcattaata gttataaatc caggaaatgt
    59221 attatataaa aatattagag agtaatatat atctttatgg taagatataa agatattatt
    59281 agaagaacat ccaaggagga ataaacatgg ttttactgat tgttgatgca caaaagttaa
    59341 taacaaacga gaggttatat aaatttaatg aatttgtagt aaatgtagaa aatttaattg
    59401 atacagctag aaaaaataat atagaagtaa tatatgtacg tcatgatgat ggagttgaaa
    59461 atgaattgac taaaggtaaa aatggctttg aaatatacga aaaatttaaa ccatgtaatg
    59521 gagaaaaaat atttgataaa aaagtaaata gtgcttttaa agaaactggt ctattagaat
    59581 acctaacaaa taatggtgaa aaagatatta ttattgctgg tctccaaaca gattattgta
    59641 ttgatgccac tataaaatgt ggatttgaac atggatttaa tataatagtt ccagcttatt
    59701 caaatacaac tgttgacaat ttttttatgt cagcagaaca atcatataaa tactacaatg
    59761 aatttatatg gaatgaaaga tatgcgaagt gtatttcatt agaggaaaca cttagaaaaa
    59821 tgaaataaaa atagagtatg aattgtatat gtacataagt tgatttcata aaaaaggatg
    59881 ttaaattcca agttaaaaat tagattaaat atattatatt atagataagg cacatcaatt
    59941 ttattttaaa ttaatgtgct ttttaattgt agtaatttat ttataatcaa tatgattttt
    60001 attatttaaa tcaatttttc ttgagtttct ttgtctaaag aaactttttt tatttataaa
    60061 atctacagtt atattatagt atctatttta gatgagttta atttttgtat caatctaaaa
    60121 caaaagttat acttaaatga aattgaaaac gataaaattc tcaaataaaa taagtattgt
    60181 ggaggtgatg aaagaatcca atgataatat gaagactaat cttaataaga aagtaggggt
    60241 taatggtagt ggacaaagaa attgtatcaa ttctccagat tttattaaat agcacattga
    60301 ttacaactaa tggattacaa gaggagtcta attcatcaaa gagacaaatt acttatagaa
    60361 taaataaaat caatgacatg cttaaagtaa aaaatgtacc acttatatgt ttaagagccg
    60421 acaaagatat tatcgttaga aaagaaacaa aaaaagcaat taaagaaatt ttagaaaaaa
    60481 actattctaa gaatacttat tattttagta agacagaaag acttttatat atgtatttaa
    60541 tgctttttat taacatggag gatatttcaa ttaatcattt tataaattct ataaaagtaa
    60601 gtaggtctac tgtaaatctt gatttcaagg atttgatacc agagcttgaa aagaagaaca
    60661 ttaaagttaa gaacaatcgt ataaatggat attacatagt tggcaatgag atggaaatta
    60721 ggagagtact tattaaaaac attatagaaa ctttgtctaa tgagaaaagt tcaagagtat
    60781 ttgatatttt tattaaagaa tataatttag atagttttga agaggctaaa aaaattatat
    60841 taaagctaat aaaaaaatat gaaattactt ttgttgaaga tagattaata gaatttatat
    60901 atatatttat ctttctaaaa gcaaggatgt attcaactag aatagttact caggaaaatg
    60961 tagatattcc taatatttca gttatgagtt caatgaagga gtataaattt gctaaggaat
    61021 tattagaagt atatgggagt gaagataaga taaagcctca caatataatg tatattagtg
    61081 cttggatttt aggtatatca gtaggaaatt atgaagaaga tacagaagat aaggatgtaa
    61141 tttcaaaaat tgtaaataaa atgatgacaa agtttggata tttaagcgga gtttactata
    61201 taagccaaga aaaaatattt aaacaattat attctcattt tagacctgcc tactatagac
    61261 ttttatttaa attacctatc tataatccga tttgtgaaaa agtaaaagaa gaatataaat
    61321 tagtttatag aattgtaagt aatgcaatga aagagttttg cggattattt ggagaagaaa
    61381 ttcaggaaga agagttagca tatcttacaa tgcactttgc aacacttttt tcaaataaaa
    61441 aagaatttga tggttttaga aaaagaatag cattaatagt ttgttcaaat ggcgttggta
    61501 gttcagctat tttacatgca gaacttacaa acttatttcc tgacttacac tttataccaa
    61561 tgtcggaatc agttggatta gaaaatataa gtgaacctgt agatattgta tttactacta
    61621 attataacgc agagggttta aaaacagatg taccagtaat taaagtaagc cctgttatga
    61681 ccacaaaaga aaaatataag attacacgtg aagtgtatat tcaattaggt gatatttttt
    61741 taagacaacc taaaatagat gaggttatgg atataataaa aaaatatgca aatgtaacct
    61801 ctgaaagtat tctttcaagt gagctattag cttattttac acagatagag aattttacat
    61861 ctaaggaagg agaggggcct atgctaagtg agattactaa tgaaacacta gtaaaaataa
    61921 aggtaaaagc taagaattgg gaggaagcta ttagacagtc agcttctgta ttagttgaaa
    61981 ataacaaagt aactgaaggg tatgtagatg ctatgattaa ctcagcaaaa gcatctggac
    62041 catacatagt aattacaaaa catgttgcac ttccacatgc tagaccagaa gctgggtcaa
    62101 aagagattgc aataggcatt gctactttag atagtcctat tgagtttggg aataacgaaa
    62161 acgaccctgt aaaatatgta ttttgtctaa gtgcaataga caataatagc catttacgtg
    62221 ctatgtctga gctagttgaa ctattggaag aagatgagtt ttttaaggtt ttagatagtg
    62281 ctaaagaagc taaagaaatt attgatttta taaaaacaca cgaattataa aggaggattt
    62341 aattatgtat aaaattttaa tcgcatgtag agcaggagtt ggctcaagtt taatgttaaa
    62401 aataaagact caacaaatta ttaaggaaaa taactttcca attgaagtag agcatggttc
    62461 attggattca ctaaatggat tcaatggaga tgctgtagaa acattaattg atgttgctga
    62521 agaattaaaa aataaaaatt tgaagtttga tataattggg attcacaata ttgttgaccg
    62581 taatgaaata aagacagcac tagaaaaatt ccttgcttca aaagagggat aaatttaaga
    62641 tgataaggag gaatatttat ggagtttatt attagtttgc tgagtacacc tgctgttttg
    62701 cttggcgtag ttgcatgtat aggattgatt cttcaaaaga aatcagctgt tgaagtattt
    62761 acaggctctt caaaaacact tatcggtttt ttgatatttg gtattggtgc ctctgctatg
    62821 acaggagcac ttcaaaattt caataaactg ttccaacatg gctttggaat tactggtgtt
    62881 atagcttcac cagaagcagc tacagcactt gcacaaggta catatggttt tgcagtttca
    62941 tgtacattaa ttcttggatt catattaaat ttagtatttg cacgtattac aaagtttaag
    63001 aatattttct ttacaacagg tcatagtttg ttcttttctt gtgtattagt tttaattatg
    63061 aaagcacatg gatttgataa tacaatcact attttaatag gtggaactat cttagggttt
    63121 atgtctgctg cactacctca attctgtcaa ccatttatgc gtgaattaac aggtggcgac
    63181 gaacaagcta ttggtcactt taacatgatt ggttatggtt tatcaggtta tattgggcgt
    63241 ttattttcaa aacataaaga tgatacaaca gagactatag aatttcctaa gtggttatca
    63301 atattcagag acttcttaat gggtctatcc atagttatgt taatcttatt ttatattgca
    63361 acattaaaag ctggtcaagc atttactcaa gaaattgctg gttcaacaca ttggttggtt
    63421 tttccgctta tagaggcatt tacatttgtg gcaggtatgt caatattgat gtcaggtgtt
    63481 cgtatgttct tagctgaaat aactgcagca tttgtaatca tttcagaaaa gtatattcca
    63541 ggttcacgtc ctgcgctaga tgttccaaca gtatttccat atgcaccaaa tgcagttatt
    63601 attgggttta tatcagcata tgctgctggt ctattagcaa ttgctattat ggcatcattt
    63661 cctagtatat ttcctgtagt aattattcca gctgctcaca tatgtttctt ctcaggaggt
    63721 acagctgcaa tatttggcaa tacttcagga ggatggagag gggctattgc tgggtcattt
    63781 gttgtggggt tattacttgc atttttacca gttatactat atccagttta tggcactctt
    63841 ggtatagaag gagcaacttt ccctaatatt gattacaatg tagttggttc gatattacat
    63901 aatattttac aatttataaa acctatttaa actaaattat ataaaagtat agtcatagtt
    63961 actatgattt caaggaggat acatcttatg gaaaatacat tatctgaatt tttagataat
    64021 atatctaatg aattgtcaga atttattgcc tcaatagatg agacatctat aaataaagca
    64081 tgcgaattaa ttcttgaagc tgaaaaaaat cacggacgtg tacatgtgac tggtattggc
    64141 aaaccaggac atgtatcggg atatatttca tcattgcttt catctactgg aacgtcagca
    64201 tatatattgc atggtacaga agcagtacat gggtcaagtg gtcaagtagt tgaaggagat
    64261 gttgttattg caatatcaaa tagtggtgaa actcaagagt taaaagcaac tcttaagact
    64321 ctaaaagtaa atggagcaaa gataattggt gtaagtggta atgagagttc atttttaaaa
    64381 aatatttctg atatattcct gtttgcaggt gttaaacagg aaggagattg tcttaataaa
    64441 gctcctcgtg cttcaatttt agctgaaaca ataatattac aatcattaag tgttgtatta
    64501 caatatgcta aaggtcttaa tacacagcag tatttgaagt ggcatccagc aggaagtctt
    64561 ggaaagtcta ttagagaggg aatataattt tatgaaatat aaagggatta tatgtgcaat
    64621 gataacacca tttgatgaaa atcaaaatat taattcacaa gcaacatgtc aattaattga
    64681 ttatttgatt gaaaagggaa tttatggatt atttattttg ggcactaatg gtgaatgtca
    64741 tgtattaaca gatgatgaaa aagttgaatt tgcaaagatt gttattaatc atacaaataa
    64801 tcgtgtacct gtttttgttg gaacaggggg taattcaact cgtgaagttg tagatttatc
    64861 taagagaatg gaagcgattg gagcaagtgc attatctatt attacaccat actttgttac
    64921 accaacacaa caggaattaa tcttacatta caaaactatt gctaattcag ttaatttacc
    64981 tattataatg tataatatgc caagtaagac tggaattaat attgaaccag agtcagtacg
    65041 tgaattatca aagataaaga atattgttgg aattaaggat agtagtggta aacttgacaa
    65101 catgaaagct tatattgaag caacgaagaa tgagaatttt agtgtgtttt caggctcaga
    65161 ttcacttatt ctggatagtc taaaagctgg tagtcaagga gctgttgcag caacaacaaa
    65221 cttcctcaca gagattggta tctctatcta tgaaaacttt atgaaagggg atttggaaga
    65281 agctcaaaaa gctcaaagta gtatagaaga acttcgacgt atactgaaac ttggaacaat
    65341 tccatctgtt atgaagaaaa ctattgtctt gaatggaatt aatgttggaa ctgcacgact
    65401 tcctgtaact gaaccaactg gggaagtact tgaagaaatt aaacgagtag ttgaaaatta
    65461 taggacaata cttaataaaa gtagtgagaa tagataatat aaatagggaa attacactgg
    65521 tatataatta atatatatat tataaaacat gactaaaatt aatgaataag taaagtggtt
    65581 tgatgagtat agaggtgata atatgaaaaa gatgaatata accaaaagaa tgtcagaatg
    65641 tggggctctt gcaattgtaa gagaagaaaa tcttaaccgt gcatgtgaaa ttgctgaagg
    65701 atgtataaaa ggtggtataa cagttataga aatgagttat accctaaata atgctggaga
    65761 aataattcaa gggcttaata aaaagtatgg tgaaacactt tgtgtgggag caggaacagt
    65821 tttggatagt gaaacagctc gtcatgctat attacatgga gctcaattta ttattgcacc
    65881 aaattacaat gaaggtgtag cgaagatttg caatagatat cagattcctt atgctccagg
    65941 atgtacatca ttgactgagg cagtagacgc actaagttta ggagcaactt ttataaaagc
    66001 tttcccaata tcagatttct atggacctaa actagtaaag gtatttaaga caccaatacc
    66061 tgatatgcca gttttagcaa gtggagggat taatattgaa aacctacata tatggttaga
    66121 aaatggagtt gatgtatgtg gatttggtgg tttattaact aaaggttctg ttgaagatat
    66181 tgctaaaaat gctaaaaaga ttcgtgaaat cattaaaaat ataagaaaaa tttaacatag
    66241 ggctgtatca tagtatctta taattactat gatacagcct ttttaatatt attcaaatta
    66301 aaaacagttt acatttctat tatattacat ataatttcag taattttatc ttaatttttc
    66361 tatctggaaa aaaatgattt aaaacttaag ttatgatata atttcattaa aagaaggcga
    66421 tttttagtaa atatgagaaa ataatgtgtt tttaggggaa aagttaaata cagatttaag
    66481 tgaacaagaa tcgataatta agatattaaa tggtgaatag ggcatcaaaa aaattactaa
    66541 attaaaagtt ttagagggag aatcttagca agtgtgtgtg aaaattcaat ttcaaacaaa
    66601 aaataattgt aaatgaaatc aatttataca cgttgaagat tcacttttat cattatatta
    66661 aaaagcagag tgaattaaaa gtgagtctaa agataggagg ataattaaaa caatgaaaga
    66721 agacattagg ttacatcgaa tgtgtaaaaa agaacaagca acaaagaatg aagttatatc
    66781 agaggatatt ctaaaatcta tgagtattgg ggtcagtaaa cattttttag atgattcatt
    66841 tacaataata tggggaaata catcattcta tcaattatta ggatatacag aagaagaatt
    66901 tttagttaaa ttttctagtt taaaagatta ttatgaacag aatctttatc attttgaatc
    66961 tatgaaaaat cattttttag aggcttatca tcatggcgaa aagagtatag aatatcaaat
    67021 ttgcatttca gcaaataatg gagaccctgt ttgggtaata atgactggta tattaattga
    67081 atcacaagat aatgagaaat caatggtcta tatattgtat tcaaaaatta atgagttaaa
    67141 aataaagcaa gaagaactat ctgatatgga aaaagagagt acagagaatt ttaaatggat
    67201 gatgtcagag tatgctggga atgtttatat tagcgatatg gatacatatg aattactata
    67261 cttaaataag cactcatgca atactttaca ggcatcagca aatgaactta ttggaagaaa
    67321 atgttatgag gtaattcaag gtagaacttc gccatgtccg ttttgtacaa attcgtattt
    67381 aaaaaaagat gacatatatg aatgggaatt ttataataaa aaactgaaac gaacatttat
    67441 aattaaagac cgtatgttga attggcaagg gcgaagagtt cgtattgaat tatcttatga
    67501 tatgtatagt gctgaatata aattagcaaa aaaagatcag gaaagagaat caattttgaa
    67561 aactattcca gcaggaatgg ttagattaga tgcaagagat tatcttacta tactttggtg
    67621 taatgatata tttttgaata tgattgaata tacaaaggaa cagtttgcag aggaacttaa
    67681 taatcaatgt ggatatttat tttctgaaga ttataaacgt agtaagaaac tggcacaaga
    67741 tttgaaggag tctggggata atgttgtatt tgaagcaaaa atatatacac gttcaaggga
    67801 agaacgtata tggactgtta cattgtgtta tatcagtggg gaagacagtt gggatggaat
    67861 accatctttt tatagtatag gtattgacat aacaaaggaa agaaaacaga ttgagaaatt
    67921 acagcatata tcagagaaag atgcattaac aggaatttat aatcgtgctg agactgaaag
    67981 gcagattaag aaatattttg aaaaaaattt aaatgttatg ggtgcactgt ttatgattga
    68041 tacagataat tttaaacaga ttaatgatac agaagggcat atgattggag atatagtact
    68101 tactgagatg gcatcaggta tgaaaaaaat aatgcgtgac agtgatgttg ttggaagaat
    68161 aggtggagat gagtttacaa tttttatgaa gaatatttcc tcagttaaag atgcagaaaa
    68221 gaaagcagaa gaactattgt atatgttccg tcatctattt cagaaagaaa aaagttttct
    68281 aaaagtaact tgtagcatag gaatagctat atatccaaag gatggtacta cattcaagga
    68341 aatatatgca agagctgata aagccctgta tcaagcaaaa aatatgggaa aaaataatta
    68401 tgtaatctac aaccaagatt attttaaaga attagaagaa tttgattgtt cttctcttgg
    68461 tacagttatt gattcagaaa aacaatacgc tgaatatcct gataatctta cacgttatat
    68521 attccgtatg ctttatcaga ctgatgatat agaccaggcg attaatatga tacttgaggt
    68581 tgttgggaaa cagtttgatg taagtcgtgt ttatattttt gaaaatacag aagatggtag
    68641 atatacttct aatacttatg aatggtgtaa tgaaggaatc tcttctcaaa tggcttattt
    68701 gcaaaacagc aattatcaag actatgagga ttatgaaaag ctttttgagg atgaattagt
    68761 tttttattgt cgagatatcc atacattaag tcctaaacta gaagaattgt tttctagtca
    68821 aggtatccac tctacacttc aatgtgcata taaagaagat agagttttca gtggatttgt
    68881 tggatttgat gaatgcacag gactacgtct atggtcacaa gaggaaataa gtacattatc
    68941 attgatttct caaattatat caatgttttt acaacgtaaa aaaaataata aacttaatca
    69001 acagattcaa caataccaga atattttaga taatattgac cagtgtattt gtgtgattaa
    69061 taaagaaaat aattttttat tatatgtaaa taaaaaattt aaaaatacat attctaaatt
    69121 ccaaattgga caaccttatt atcttgattc tatagatatt cataagatat ccattatatg
    69181 gaataaaaaa gaagcatacc tatgtactag cacgatttaa tggtaaagta aagtttatat
    69241 tggacttaaa ttaaatttcg ttttaaataa gatattattt tagttgctta ctgaaaagac
    69301 tagaatctat attaatgaat tatactaatt ttataccact attaaaatat tgtttgctaa
    69361 aaataaaaaa tagccttaaa acagttgatt ataaaaaatt aactgcttta aggctatttt
    69421 tatctttgtg acgaaataga cacttgccat ttagaatgtg acaatatagt agttgtttag
    69481 aaaaggcaca aagcattata ctttaactaa agtaaaatgt attttgcttt tagaataaaa
    69541 acaattagga gggagctata attgaaaaag ataatatatg tattatttac aattgaacta
    69601 ttaatagtat ctttattagg tctaaatata gttaaagata atgaaattaa taatatttta
    69661 tataacgaga caacaagtat atctgtaatg tttaaagatt ataaaaagtt aaataaaaat
    69721 tatagtaagt ggattaaaga tattgcagat gaaaataatg tgactatatc aaagtatgtt
    69781 tttaataata atgaaaaatt atctatatat acgactgata caagtttgaa taacaatata
    69841 aagttaaaat caggaaaatt tcctaatact aattcaaatg aatttattag taatataaac
    69901 actaataata gtaatcaagt aggtaaattc tcaactatga gtaaagacat atcaatttta
    69961 attagggatt ttggtaaaat aagtgaagtt ggagaatcag gaatcttata tatttcaact
    70021 caaaatgaag atacaataaa taaaatatta agagaactaa atgtagacag aagtattttt
    70081 gtagcaagat tacatgatag atatgttaac tcaaatttat acttaaatcc ttcaataatt
    70141 agagatttaa tactaattat cctttgtttc ttggctacaa taatacatta ttcaattagt
    70201 gtgagtagag aggcttctat tttaagatta aatggatact ctaagctaga tataattgct
    70261 agagttataa aaaatatact tagaattatg attttatcat ctgtcacagc attattaata
    70321 tatataatct atattttaaa attaaatata ggtgttagag catgcttata ctttgctatg
    70381 ttttcgatta tatgtatttt gataaatatt ttgatatcta tattaatagt tggttttaat
    70441 agtagaagtt ctaaatatgt gctatctcta aatggaaaaa aatcttatgg ttttgtaaat
    70501 ataatgcatt tcacattgaa aattgtattt gtatcatttc ttatattatc cataaataac
    70561 tgtattttaa attataatat gctacaaact cagttaggaa atctatctga gtgggataaa
    70621 gcaaaaaatg tttataatct tactcttaaa gatacaggcg aacaatcttt aggaaaggaa
    70681 gaagtaatta aaaatgccaa gattaaaggt ttctataaaa atttggtaga agaaaaagat
    70741 gctttcttaa tagattcaac taattttgaa aaacttgaag atggaagtta catgtatgaa
    70801 ataaattcaa agggtaaaaa cccagaagta agtcaatatg gtaaaaatat aaaaataaat
    70861 aaaaactatc ttaaagttaa ccctatttac tcaaatggca aagaaatatt taatctaatc
    70921 gattatgacg ataagactat aaatttatta gttcctaaaa agttacaagt acatgaaaat
    70981 gagattaaaa aagagtttag agaattattt tattttgaaa aaatagaagt agaaaatatg
    71041 tacaataaag agttaaatag agcgttaaac aaaacaaaga agtcagattt aaatgttaat
    71101 ataatatatg tagataataa tcaaagctat tttacttata actcccttgt tatggatgac
    71161 aataggaatc tcattgatga cccaatagca atagttgaca tagataatat tgatgattct
    71221 ttttatcttt cttatatatc tagatgtgta tattttaatt ctaagaaatt agatgccctt
    71281 gcagatataa gtagcattat tgaagctcaa ggtgtagaag cgcatatcca aagtttacat
    71341 gctgtttata atgagtatgg tttagaaata aataaactag aaaaatattt gaatagtgaa
    71401 atttttacta taataataat agcaatatca aacttaatga ttacctacaa tatagtagct
    71461 agctactatg aaag
//
LOCUS       DAEMGG010000005        67337 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEMGG010000005 DAEMGG010000000
VERSION     DAEMGG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.005"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(376..1098)
                     /locus_tag="KPK24_000304"
     CDS             complement(376..1098)
                     /locus_tag="KPK24_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG2298020.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1157..1834)
                     /locus_tag="KPK24_000305"
     CDS             complement(1157..1834)
                     /locus_tag="KPK24_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG2298021.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1862..2677)
                     /locus_tag="KPK24_000306"
     CDS             complement(1862..2677)
                     /locus_tag="KPK24_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2298022.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2903..3113)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3496..5562)
                     /locus_tag="KPK24_000307"
     CDS             complement(3496..5562)
                     /locus_tag="KPK24_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG2298023.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(5639..8668)
                     /locus_tag="KPK24_000308"
     CDS             complement(5639..8668)
                     /locus_tag="KPK24_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2298024.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCAIILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(8721..10181)
                     /locus_tag="KPK24_000309"
     CDS             complement(8721..10181)
                     /locus_tag="KPK24_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG2298025.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(10273..10647)
                     /locus_tag="KPK24_000310"
     CDS             complement(10273..10647)
                     /locus_tag="KPK24_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298026.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(10823..11563)
                     /locus_tag="KPK24_000311"
     CDS             complement(10823..11563)
                     /locus_tag="KPK24_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG2298027.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(11584..12372)
                     /locus_tag="KPK24_000312"
     CDS             complement(11584..12372)
                     /locus_tag="KPK24_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG2298028.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLTFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(12460..12550)
                     /locus_tag="KPK24_000313"
     tRNA            complement(12460..12550)
                     /locus_tag="KPK24_000313"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12514..12516),aa:Ser,seq:gct)
     gene            12809..13573
                     /locus_tag="KPK24_000314"
     CDS             12809..13573
                     /locus_tag="KPK24_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG2298029.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(13854..14969)
                     /locus_tag="KPK24_000315"
     CDS             complement(13854..14969)
                     /locus_tag="KPK24_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG2298030.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(15010..16536)
                     /gene="glpK"
                     /locus_tag="KPK24_000316"
     CDS             complement(15010..16536)
                     /gene="glpK"
                     /locus_tag="KPK24_000316"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG2298031.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            16829..17926
                     /locus_tag="KPK24_000317"
     CDS             16829..17926
                     /locus_tag="KPK24_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG2298032.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(18379..18891)
                     /locus_tag="KPK24_000318"
     CDS             complement(18379..18891)
                     /locus_tag="KPK24_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298033.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KMDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            19444..20325
                     /locus_tag="KPK24_000319"
     CDS             19444..20325
                     /locus_tag="KPK24_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG2298034.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSIKDVEKFINFFSAIPAKDTDDTIKISEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(20638..21417)
                     /gene="hisJ"
                     /locus_tag="KPK24_000320"
     CDS             complement(20638..21417)
                     /gene="hisJ"
                     /locus_tag="KPK24_000320"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG2298035.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLILGSDSHCAEEINKYDIV"
     gene            complement(21963..22313)
                     /locus_tag="KPK24_000321"
     CDS             complement(21963..22313)
                     /locus_tag="KPK24_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG2298036.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(22329..22745)
                     /locus_tag="KPK24_000322"
     CDS             complement(22329..22745)
                     /locus_tag="KPK24_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG2298037.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(22764..22955)
                     /locus_tag="KPK24_000323"
     CDS             complement(22764..22955)
                     /locus_tag="KPK24_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG2298038.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(23106..23921)
                     /locus_tag="KPK24_000324"
     CDS             complement(23106..23921)
                     /locus_tag="KPK24_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG2298039.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(23968..24906)
                     /locus_tag="KPK24_000325"
     CDS             complement(23968..24906)
                     /locus_tag="KPK24_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2298040.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(24903..25775)
                     /locus_tag="KPK24_000326"
     CDS             complement(24903..25775)
                     /locus_tag="KPK24_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2298041.1"
                     /translation="MNKNLSEKMEIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(25800..27728)
                     /locus_tag="KPK24_000327"
     CDS             complement(25800..27728)
                     /locus_tag="KPK24_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG2298042.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(27840..28217)
                     /gene="gcvH"
                     /locus_tag="KPK24_000328"
     CDS             complement(27840..28217)
                     /gene="gcvH"
                     /locus_tag="KPK24_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG2298043.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(28304..30430)
                     /gene="cdhC"
                     /locus_tag="KPK24_000329"
     CDS             complement(28304..30430)
                     /gene="cdhC"
                     /locus_tag="KPK24_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG2298044.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(30487..31293)
                     /locus_tag="KPK24_000330"
     CDS             complement(30487..31293)
                     /locus_tag="KPK24_000330"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG2298045.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(31343..32710)
                     /locus_tag="KPK24_000331"
     CDS             complement(31343..32710)
                     /locus_tag="KPK24_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG2298046.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(32741..33685)
                     /locus_tag="KPK24_000332"
     CDS             complement(32741..33685)
                     /locus_tag="KPK24_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG2298047.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(33705..34475)
                     /locus_tag="KPK24_000333"
     CDS             complement(33705..34475)
                     /locus_tag="KPK24_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2298048.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(34531..35916)
                     /gene="lpdA"
                     /locus_tag="KPK24_000334"
     CDS             complement(34531..35916)
                     /gene="lpdA"
                     /locus_tag="KPK24_000334"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG2298049.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(35960..36841)
                     /locus_tag="KPK24_000335"
     CDS             complement(35960..36841)
                     /locus_tag="KPK24_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG2298050.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(36877..37551)
                     /locus_tag="KPK24_000336"
     CDS             complement(36877..37551)
                     /locus_tag="KPK24_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG2298051.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(37588..38460)
                     /locus_tag="KPK24_000337"
     CDS             complement(37588..38460)
                     /locus_tag="KPK24_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG2298052.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(38552..39184)
                     /locus_tag="KPK24_000338"
     CDS             complement(38552..39184)
                     /locus_tag="KPK24_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG2298053.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(39289..40965)
                     /locus_tag="KPK24_000339"
     CDS             complement(39289..40965)
                     /locus_tag="KPK24_000339"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG2298054.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(41214..41993)
                     /locus_tag="KPK24_000340"
     CDS             complement(41214..41993)
                     /locus_tag="KPK24_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2298055.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(42303..44222)
                     /gene="cooS"
                     /locus_tag="KPK24_000341"
     CDS             complement(42303..44222)
                     /gene="cooS"
                     /locus_tag="KPK24_000341"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG2298056.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(44829..45740)
                     /locus_tag="KPK24_000342"
     CDS             complement(44829..45740)
                     /locus_tag="KPK24_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG2298057.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(45856..46434)
                     /locus_tag="KPK24_000343"
     CDS             complement(45856..46434)
                     /locus_tag="KPK24_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG2298058.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRAIDEIPE"
     gene            46660..47583
                     /locus_tag="KPK24_000344"
     CDS             46660..47583
                     /locus_tag="KPK24_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2298059.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQGSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(47755..48897)
                     /locus_tag="KPK24_000345"
     CDS             complement(47755..48897)
                     /locus_tag="KPK24_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2298060.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(48915..50669)
                     /locus_tag="KPK24_000346"
     CDS             complement(48915..50669)
                     /locus_tag="KPK24_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG2298061.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            50805..51011
                     /locus_tag="KPK24_000347"
     CDS             50805..51011
                     /locus_tag="KPK24_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298062.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(51016..51939)
                     /locus_tag="KPK24_000348"
     CDS             complement(51016..51939)
                     /locus_tag="KPK24_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298063.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(52193..53893)
                     /locus_tag="KPK24_000349"
     CDS             complement(52193..53893)
                     /locus_tag="KPK24_000349"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG2298064.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(54303..54716)
                     /locus_tag="KPK24_000350"
     CDS             complement(54303..54716)
                     /locus_tag="KPK24_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG2298065.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(54782..57160)
                     /locus_tag="KPK24_000351"
     CDS             complement(54782..57160)
                     /locus_tag="KPK24_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG2298066.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            57479..58930
                     /locus_tag="KPK24_000352"
     CDS             57479..58930
                     /locus_tag="KPK24_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG2298067.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(59134..61647)
                     /locus_tag="KPK24_000353"
     CDS             complement(59134..61647)
                     /locus_tag="KPK24_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2298068.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTSQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKALDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(61870..62691)
                     /locus_tag="KPK24_000354"
     CDS             complement(61870..62691)
                     /locus_tag="KPK24_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2298069.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(63209..63685)
                     /locus_tag="KPK24_000355"
     CDS             complement(63209..63685)
                     /locus_tag="KPK24_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG2298070.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(63720..64298)
                     /locus_tag="KPK24_000356"
     CDS             complement(63720..64298)
                     /locus_tag="KPK24_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG2298071.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGNEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(64402..67002)
                     /locus_tag="KPK24_000357"
     CDS             complement(64402..67002)
                     /locus_tag="KPK24_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG2298072.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHSNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNVFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        20953 a        12585 c         7404 g        26395 t
ORIGIN      
        1 ttactataaa agttaaattt ataagcttac ttgttataaa aataactttt ataatttatt
       61 tcaaaaaaaa tattgatttt tgtgtcgcat tttgttataa taaattaagg ttatccccct
      121 ggtaacccta gttagttaat atatctattc ccaataccct ttcataagga tatattttat
      181 atttattaaa aatcggatta gtaacggaaa ttaattcggt ggaaaaaaag agcttacccc
      241 gagctctttt tttttctgca aaaaatcgta aaaataaagt taatattaat ataactttaa
      301 ttttaaaatt aatattaacc atcactaaaa aaggatgtat tgaatttcta atatcaataa
      361 catccttttt atatattata gcacttttgc taaaaacgct tttgttcttt catgttttgg
      421 atttccgaaa acttcttctg gagtattatc ttcaagtata cttcctccat ctataaaaat
      481 aactctatct gcaacttcct tagcaaatcc catctcatga gttacaattg ccattgtcat
      541 accttcttta gcaagttctt ttataacatc caaaacttcg tttaccattt ctgggtcaag
      601 agcagatgtt ggctcatcaa acaacatcat atctggttcc atagcaagtg ctctagctat
      661 tgctattctt tgcttttgtc ctccagataa ttgtgatgga tatgaatctt ttttatctaa
      721 aagaccaact ctactcaaca aaatttctgc ttttttttct gcttcttcct tagtcatctt
      781 ttttaattta attggtgcta atgttatatt atctaaaata gtcttatgtg ggaataaatt
      841 aaagttctga aacaccatac ctatattttg tctaacctca tcaatattag tttttttatc
      901 gaccaaattt tttccttcaa atattatttc tccacctgta ggtatttcta gtaaattcat
      961 acatctaaga agtgtacttt taccagaacc acttggacct actataacca ttatttcgcc
     1021 tttagcgatt tctaagtcta tattttttaa tacatttaaa tctccaaaag atttacttaa
     1081 attttttatt gtaatcatat tatcactttc ctttttacta ttctatgttt tccctaaatg
     1141 ctaaacatgc tctcatctag ttagacacag atagtttctt ttctaataat ccaactaatc
     1201 ttgatagagt aaatgttata acaaagtaaa ttgctgctgc tactattaat ggctctaatg
     1261 ctctaaatgc tgcatttttt actatatcag cagtacgcat tatctcaaca actcctatta
     1321 cagatattat tgaagattct tttacaagtg taataaactc atttgctaat gaaggtaata
     1381 tattttttat agcttgaggt attattatat atctcatagt tgaccaataa tttaatccta
     1441 atgatctact tgcttccatt tgtccattat caactgattg tatacctgat ctaagtatct
     1501 ctgccacgta tgcaccagag tttactgata atgcaaatgc actagctata aattcacttg
     1561 gtataccaaa caacataggg aatcttattc ctaattgagg taaaccaaac catactatat
     1621 atatctgtac taataatggt gtccctctta aaacctcaat atagattgat gatattgctc
     1681 ttaatacttt gctctttgat atttttgcca tacagataat tatacctatt ataaatccaa
     1741 agcataatgc cacaagagaa attgatactg tcacacctgt tccttccaaa aaagaagtcc
     1801 caaatctact taaaaaacta aaatctaaac tcataatttc atctcccaca taactttctt
     1861 actaatttga agcagcttca gtagatgctt catttaaaaa ttcttctatc ttcttgccat
     1921 cttgtaattc ttttatcttt ttgtttaata attctataaa atctttgtta ttgtcagatt
     1981 ttttgattgc tgctgccata ccttctgtta catctttccc aacttcagta tttgccattt
     2041 ttattccatc atatttttta acatttatta aagaaactgc ttcattagta acaactgcat
     2101 ctatgtttcc attttttaat tctgtaatta aatcaggtat tgctttcaaa gattttgtag
     2161 ttgtcatttt taaagtgtta actacatatt cttcttggat acttcctgct tgaactccaa
     2221 ctctaagttt ctttaaatca tcttcagttt ttactttgtc tatatctgca tctttaacta
     2281 taactgcatt tctacttaaa taatataatt ctgaaaaatc aacactcttt tctctctctg
     2341 gtgttggaga catacctgca agaacaatat caacttggtc tgcatttaaa gcacctatta
     2401 atccatcaaa tgacatatcc ttaatttcaa cctttacacc aagctctttt gcaaattctt
     2461 cagccaacat tatatcgaat ccttttattg aatcttttcc atctactacc ttatgaaatt
     2521 caaatggagg aaattctgca ctagtaccaa caactaattt tccattgtct tttatttgtt
     2581 ctaactttgt tttttctcct ccaccactac atcctactag cgataatgtc aatcctaata
     2641 ctaatcctaa acttaataat tttttaaata ctttcatttt aactacccct ctcttttaat
     2701 tataatttcc ctcagatttt aatacttaaa tgtttcgagt tacaaaaaaa gtctctcagt
     2761 ctttaatgac taagagacga ctttaccgcg ttaccactct tattgataaa tacttatatt
     2821 actttcaaaa tttgtgactg ttagctttta agctgtatgg atatttttat tttaaaatca
     2881 aaaaagtccc ttagctttac agctaagaga cgaattatat ccgcgttacc actctttttg
     2941 ataagtaagt acttatcctc tcaatccctt aaggcgggta aaccaattat catactaaag
     3001 tttctgataa tctcctccag agctctcttc acatagactt cattgttagg cttacaccaa
     3061 cccctaactc gctgtaaatc catatatgct actttctcta tcattggatt tgtttcctta
     3121 aattgttaat acaattgtac ttccttataa tatttttgtc aataagtttt ttattttttt
     3181 aataattttt acttgtaaat tatctaaata ctactgttta tatttattga tattatttgt
     3241 gtaattataa atagtatatt tcatattttt agctatttct taaataatac taaataatat
     3301 ctttcacatt gttgcttttc tttaataata ctaaataata ttttccacat tgttgctttt
     3361 tctaaacaac tataaataat atattaaacc cttatttctt tttctaataa gttaaataat
     3421 acattttaca catatctatt tgtcttgcac ataataaata atacattata tttctatcta
     3481 aatttagatt ctaaattaaa tagtataaat tatattcttc aataaacaag ttttcaaatc
     3541 caagattcgc tctctatctt taaactttat tgtaaacata attccatctt ttttaattat
     3601 ttctccttca tgaaattttt tatggtatac cttatcccct acaccaatac tgtcaatttc
     3661 ttttttcgtt ggagatttta tatcattaat aaataatgat ttatcaactt tcttaccata
     3721 cttatttaaa gtagagctta tacataactt ttcttcagct cttgtaatag caacatacat
     3781 aagtcttctt tcttcttcta attgttcttc ttttttctct tccttacaaa tatcatatga
     3841 tttttcatga ggtattgttc cttcatttac acctataata taaacatttc taaattctaa
     3901 tccttttgca ctatgcatag ttgtaaatat gacaccctca gtctgcttat tcttgttatt
     3961 ttccgttaac tcagtcttta ctctatcaat atgctctaaa aactctgtaa cagtattaaa
     4021 attagttgct gaactctcaa gttcatttaa tatctctaca agtccattag tctttatctt
     4081 tctgttgcta caataatcta aaacatatct atcataatca agactagttc taatataaga
     4141 tattgcattt ttaggattta aagtatttaa ataacttaaa tctatttcca aatcatttat
     4201 tgttttaact tgtttaggat gaagattaca cttatttatt aatgctgtta taaagtcttt
     4261 ttcatcctta accatattta tactgtcttt ggatatatat ctaaaaggct tgtttataat
     4321 tctcaaccaa tctttatttg attttggatt aatacctatt cttaaatagg ctagtatatc
     4381 ttgacttgcc caatggtcat atattgtaat tactgagtct tttactacaa atggaatcct
     4441 catatccata aatacatcta ctaatgctct tgactgtata ttagttctat aaattactgc
     4501 aaaatctgaa tactctacat aatctttttg tatttcgtca attatttccc tggcaatata
     4561 taaggcttct tcctctgaat catgtggaga aatataggta acacttcctc catccccctg
     4621 actacactta atgatttttt catatctatt tttatttttg tcgataagtc tatttgcagt
     4681 atgcactatc tcacttttag acctgtaatt tatatctaat attatttttt tagtattatt
     4741 aaagtattgt tcaaattcaa gcaaaaaatc tggtctagca cctctaaatc catatatact
     4801 ttggtcttca tctccaactg caaatatatt gtttaatggg ctacaaatta actttaaaac
     4861 ttcaaactgt actttattta tatcttgaaa ttcatctatc aatatatatt tatacacatt
     4921 tctaaccatt tctagtgcag acttattgtt caaaagtaaa taataagttc ttataagcat
     4981 atcatcaaaa tctattttat taactttaga tttttgttct tcgtataagt tgtatacttt
     5041 taaaaactca tcttttgtta atacttctga attaaattca tttttatcca taagttcatt
     5101 ttttacatag gaaatttcat ttataacttg tcctatattc tcatcatcat cagcattctc
     5161 tatatttaaa ctttttagga ttgccttgat tgtcattcgc tttgactttt catcaaatat
     5221 actgtctaag ttgtatcttt caaagtatct tagaatttta aaaaatacag agtgaaaagt
     5281 accataggtt accttattaa gtcttatgtc atcacttaaa ttcaaagctc tatttttcat
     5341 ttctattgaa gatgccttgg taaaacttat tgccaagatt cttgttggtg ctatgttttt
     5401 gtttacaacc atattggcta ttctatatgt aattactctg gtcttacctg aaccaggccc
     5461 tgcaagtatc atgcaaggcc catctatatg ctcaactgct tctaattgat tttcatttaa
     5521 ctttcttata tctatatttg ttgtgattat tagctccacc accttgattt ataattatat
     5581 tattttcttt ttaattataa actaaaagtt cataaaaata aatttaattt ttcctctatt
     5641 aaaccacatc attcctattt tcatgacata tcttgagttc ttttttaatt ttgtcgctta
     5701 tatcttcagg tataaatgat ggtttactaa tatagtagcc ttgcatataa tctactccaa
     5761 gattaattag tgtttccatt tcaccttttg tttctacacc ttctgctatc actttaatat
     5821 ttctttcgtt agaatatgag ataagatttt ttaatatttt ctgtctgttt tcatctttat
     5881 caatacctct tacaatagac atatcaatct ttacatagtc tggcattata tataatagta
     5941 cagcatctcc gttgtaacct gtcccaaaat catctaaagc aatttctata ttccaatggt
     6001 ctatatatgc tttctttttt tcaataaaat cttcattaat cttatcattc tctgttattt
     6061 ctagtactac tctatttaaa tattcaccat ataaatcttc aatttcctca aaatctactt
     6121 ttgacaaatt attattagga atagaattta agaacaattt gcaatcgcca aatttttctt
     6181 tgtatttttt aaatgattta agtgctttaa accatgtaat acgctcaatt tgatataatt
     6241 tagactgtga acgagctaat ctaatgacat ctattggtga actaaaagtt tttattttag
     6301 gtctcataag tgcttcatat gcaaatactt tacctttaca atctacaata ggctgaaatg
     6361 catactcaac taattgacca tcaattaact tgtttaattc ttctctatta ctcaataaga
     6421 atgaatcctt attatatata ttaatatcaa attcactaac ttcacccttg attgtatttt
     6481 taatttgata catagcaaaa tctgcgtatc tcattaattc atcatagttg gttgagtcat
     6541 cagggtacca ggaaattcct gctgatgccc ttattttaaa ttctgttcca tcatgaagtt
     6601 ttaataaggt attattcatc tgtttcttaa cagaattaat aatatctctt atactatctt
     6661 tgctttcata tccatatata aatacataaa actcatcacc tgaaagtcta gatactatac
     6721 cattccatga tataaacttc ttaagtatgt ctgcagcaca cctaatatat tcatcaccag
     6781 catcatgtcc atatgtatca ttaatatatt ttagattatc taaatcccac ataataaatg
     6841 ctgctatctt aagttcatct ttattcttaa atttttcttg tatctgtgaa tgaaaagctc
     6901 tcctatttaa aagattcgtt aaaatatcaa aatctctttc atattctatc ttccttttct
     6961 caataaattc ttttgtgaca tcaacaacaa caccaagaac ctttagctca tcttcaaaga
     7021 tattcattct tatccactta agttttttat ctttagattc aaacttatat atatatgtat
     7081 tttctgtttc ataactttct tgcaaatatt tatctagtcc ccttaatttt ttcaggaact
     7141 tatttttttc catatatcca tattcttcaa atttgatttc agtagtaaac acgctaaaaa
     7201 attcttttgt acaaaaaaca ttctctgcgt ttttgtataa ctcaaaagcc ccaattggca
     7261 tatctaccat ttcaataatc tgtgataatt ttgacgaaga atctgcaaca tttgcactga
     7321 gcaactctat agctgaagat aattcatcta tttcagatat atttatctta tcaagattta
     7381 ctggttttga tggattactg tttctaactt tgttggctaa aagaacaatt ggttttgtaa
     7441 ataatcttga tgcaatgtaa atacctatta gacctattaa taaagaaatt gctatagata
     7501 ttataactaa tttctctacc ctttttgaaa atgacaaaag acttttttct tcaataatac
     7561 ccaaaagagc ccaacgttca ttttcaaatg gagtattgga gttatataaa tccaagtatt
     7621 gtacactagc ataaatgttg ccattaccac attcattatc tttattttta atcttatata
     7681 tatttttata cgattccttc tgctctaaat tagaatgtaa accatcccaa aatttttctt
     7741 ttaatgagta tccatttgaa attacatttt caaaggccat atcattattt ctatctacac
     7801 ctaaaatata attacccttt ttatttccat ttatctctga gtatggaagt aattctctta
     7861 aataatttac acttaaatct attccaagta ctccatatac agttccatct tcataaataa
     7921 ggggaactgt atatgtcata agcctatcac ctgcatttgt caataaaaac ttacttcccc
     7981 agtatcctaa attagaactt gatacatttg gattatcaat tgcagctcta aatggtttat
     8041 aaaaaaaatc atctccatct tgctcatttt cacttttaaa tgaaaactta ggttcccaat
     8101 aactgtccat agatattcca aaacttcttg ctatatctga tgaacctctc tcaattaaca
     8161 aatcagaatt atcatttgga ttaaacttag ggtctaaatc ccttatatac aatcctgtcc
     8221 tacttatttc acctttacta tctattgact tatcgttaat atttttgtca cttaggatta
     8281 taaaagctcc tgtaacagaa ttttttctca aaagatatat taaatcattt gatatgcttt
     8341 ttataatatc tttgttaatt tcttcattgt tgcctacatc ctcaattaag gcattttttt
     8401 catttaatac tttttttaca gaggaattaa ttgtagcttc cgtttcactt gtatttgacc
     8461 atctttgtaa catttcattt tgtatataat ttttacgatt aattactctt tcatttaaga
     8521 tgtcaaacga attatcattt aatttcttaa tagttcctcc ccataaaatt atggcacaaa
     8581 ataaacttgt ttgaattagc ataacaataa ttaggggaaa tatcatcttt ttcattattg
     8641 aattgttttt tcttagattg ttttccatac ccattccacc tatacctttg tgctcgtcaa
     8701 cacactaact gccaattcat ctattttttt atagcatttt caagctcagc cttaaaactt
     8761 tcaaaccaag ttttaaaatt tttatcatta ttaaattgtg ctactgcatc cttcttagaa
     8821 gttccacttt ttactaattc atttatttta tttgaatctt ctttagcttt atcaagcatt
     8881 gaatattcaa gtatttgtct agctttagtt ccaccatcaa atgctttatt agtatataaa
     8941 ttacaactgt taatctgttc aatagatgag gctattgaac cttcaacttt atcagataaa
     9001 ctcaaattat ctttcttcat aacttctttt ataacatcca aatcattcgt ttttttcttt
     9061 actggtaagt atccagaagc aatagagaat tttatattag gttctttatc tgtaaaccat
     9121 tttaaaaatt ctactgatgc atattcttct tctttagtag attttgttac agccattcct
     9181 gcaccttgtt gtacagcata ttttccttct cctttaaaac gtggtgcttg aaaaacagat
     9241 gtttcaatat tataactctc attatcattt agcattactc tatctggaaa gtatgctgct
     9301 cctgaactag aacctacaag agctaatata tcacctgttt ttgcatcatc agaacgaaac
     9361 ttaccataag aagcaaaata tccatttata tatggtacat agaaattatc ccatagtttt
     9421 ttcataactt tttcatcaat atttagtgta actttaccat ctttaacaga aaaaatttct
     9481 actcctagtt gtttacttcc tataataata taatttgcta aagcatctct tccaaaaaat
     9541 gctttaccat cattaggctc tttcgttaaa ctatctgtcc attcataata tttttttgat
     9601 gtctctacta atccttcaat tgtttttata tcatttatat ttgaatttgt agcttttgca
     9661 aatttatccc aatcagtttt atttaacatc aaaatttcag ttgattttgc aattgggaaa
     9721 agtttaaact catccttaga atttattctg ccttcttcta catattcgtc tatgtattca
     9781 tttaattctt ctttacttat gtacttatcc atatctacta gtaaccctaa tttatccatt
     9841 tcatatgcag tatctgagta tgctgcaaat atatttggta catcttcaga accaactttt
     9901 ttatttgcag aatctaaaac tttctctgat aaatcaacta cattaccttg actgaatgct
     9961 tcaacaataa ttcctttttc agcacctaca gtgtcattaa attccttaac aagcttatca
    10021 aaagccattt tctgctgtcc attataataa tgccaaattt gtattgatgt cggattttct
    10081 gggtctaact tataattatc ttccttcttt aaactacatc ctgtaagtaa taaaacaatc
    10141 attgatgtga taattaattt tgcaaactta cacctattca taaaatctac tccttcaaat
    10201 tataaaattt ataatagcat taaaactgta acttcactat ttatattaat tatagaacgt
    10261 ttattgatat atttaaacag gatttacaac atatttaaaa gcaattaaga aacatactat
    10321 tacaaatata gaaaataaag cttttacata atttttattt ctaaatgcac caaatgacac
    10381 caatccttgt tgaatagcta ctgtaattac taaaagtatc attattatat taagttgaac
    10441 cagtttaaat aagtaaagca atgaaaacac tactagaagt acacataata caatactaga
    10501 tttattaata atatcatcaa tcttatctac tttcttagat tgcttattaa ttttatttaa
    10561 tttttcttta tttaaattct caactttttt attatacctt tctcttgttt tatctttata
    10621 tttagtattt ttatttttat ttcccattta ttttccatcc tctataaatt ttttaattga
    10681 gcttatacat tatttaaata ttatatattt aagataccat atattcctac aaaaaggtaa
    10741 cttactatgt atctattttt gattttcgca taaaaaagcc atctccttta gaattaattc
    10801 tgtattgaga cagctaattt aactatttat cctttgaaac attttctaca ttatcatatt
    10861 tactatcaac taaaactata tcaactcttc tattttttga cctaccttct tcagatgaat
    10921 tttctgctat tggtctaaac tcaccatatc ctacagcaga taatttatct ggtgctatac
    10981 ctgcattgtc aataagaagt tgaacaacat tggttgctct cataactgat aaatcccaat
    11041 ttgacttaaa ctctgaattt tttatagaca tattgtctgt atggccttct actctcatgt
    11101 aactattcat ttcatttaac atcttaccta tttgaataat cttatctcta gaattatcct
    11161 tcacaatagc tttccctgta tcaaatagta ttgtatcttt aaggcttatt actaatcctc
    11221 tatcttgaac tcttaatgat actgaattag ccataccatt ttcgtttaaa tacttctgaa
    11281 tttcttcacc aattttttta aacttagcat tttgtaaagt atctaaatca ttacttcctg
    11341 gttcaactac tggttctatc ttgctattac caccttcaat aactccagaa gaaccaccaa
    11401 aagcagaatt tagagattga gataattgtt tatatttctc cgcatcaaca ttactcatag
    11461 aatacattat tacaaagaaa atcataagta atgttatgag gtctgaataa gttaaaagcc
    11521 atcgctcatt gttttcttct ttttcatctt catcatacaa cataaacatt ccatcctttt
    11581 atattattca gctctaccac caatttcttc aagttcaatt agttcttttg aatttaaaaa
    11641 tatttttaat ttttctgtca ttaaattaga attatcaccc tcaagtatgg ataatattgc
    11701 ttctatcacc aaaagtcttt cacttacttc ttgttgattt atagccttaa gttttgatgc
    11761 tattggtaac catattaaat tggcagaacc aacaccatac aaagtagcca aaaatgcaac
    11821 agaaatttga ggtcctaatg catctggatt acttgaaaga ttacctaaaa tatgtactaa
    11881 tcccataact gtacctatta tccccagtgt aggtgcatat ccacctgctg aatcaaaaat
    11941 tgctattcct tctttatgtc gttttgatgt tatctgtatc tgtgattcca atatatttct
    12001 tatagtaact gcatccactc catctaccat aagttcaagc ccttttctaa taaaagggtc
    12061 taattcatca ttctcactta tttctttgtc aatactaaga agcccctctt tcctagcctt
    12121 tgttgcaatt tctttgaaat agattatatt ttctatgtta ttaggctttt tatttttaaa
    12181 agttaataac attatcttta tagacttttt aaacacaggg aatggagtcg aagctccaac
    12241 agctcctata gttccaccaa acacaacaag tgctgatgaa aatacaaata atgctccaac
    12301 atgtccccca tcaataacaa aagcaactac caaagatgct atccccacta tgaaaaaaat
    12361 taaagttgtc atctattccc tcctttcttt agttatacat atctaatcat ttatgacatt
    12421 ataaaaagac tatctttaat aaaaatagcc tttaataatt ggcggagaag gagggattcg
    12481 aaccctcgca ccggttaccc agcctaatcc cttagcaggg gatcctcttg agccacttga
    12541 gtacttctcc atatctctat actagtaaat tatataataa aatcatattt tagtcaattg
    12601 ttttataaaa tttgagttat tttgaattat taaacatcta tttttacttt tttgcttaaa
    12661 tgtaagtttt aaatatggta aaattatctt gcataacact tataatatta tttgataaat
    12721 atacatacat taacaaatat acctaaactc tgacttaatt gtaagttatg ccaaaactaa
    12781 aataatacta gtccaaggag gatattttat gttaccacag gaaagatatg agaagatttt
    12841 aagtattctc aaatcagata atataattaa aataaatgac atagttcaaa tgtttgatat
    12901 atcaatagaa actgcacgaa gagatttagc ccatatggag agtatcggtc ttttaaaaag
    12961 agtttatggt ggtgctatac cagttatagc aagtagcgta gaacttaact atgttaaaag
    13021 aaaaaaaata aactcaaacg ataaaaaaca gattgctatt aaatgtagtg agtttataaa
    13081 tacaaatgat actattttta tggatactgg aactactgta cttgaagttt caaaaaattt
    13141 gaaagataaa aagaatctaa ctgtaataac caactcaatt ttagttgcaa acgagcttat
    13201 gaatactaat ataaaagtat atgtgcttgg gggctatctt agagaaactg aaggttcttt
    13261 ttctggtccg cttacactgt cagctattga acaatttaat gttgataaag ctattttagg
    13321 cgctggaggt atcactcttg aaaatggaat ctctgactat cacttagaag aagcacttgt
    13381 tagaaaaaaa atgattgaaa ggtctagaaa atctatagta gttgtcgaac ctcctaagtt
    13441 tggtataaat tcatttgcat cagtagctcc tatagaacat atagatacta ttattacctc
    13501 tgctagtata gaccataata taataataga gttttctcaa aaaggaatac ctttagtcat
    13561 agctgataat tagcttatat gattgatttt tagttccttg atgtaataaa ttaaaattaa
    13621 ggagccaaat aattatttat ttagctccat ttatgtctaa actatacttt atattttaat
    13681 tatgtaacta ctttcagtta gcttatcatt tttagttatt tgattgccat tttatttaat
    13741 ttataagttt tagttattcg attatttttt aattgtctgt ctttttaatt tatttttagt
    13801 tatttaaact cctactttaa aataatttaa atttgactta agtattgttt attctacact
    13861 gcatctttct tcattttaat actttgttct tcttccaagg ttaaatcttt tgtcataagt
    13921 atagcaagtg caattgccaa tactcctcca accaattttc caattacaac aggtgtaatc
    13981 atctctggaa ctactcctgc tgtaaatcct aaatggtccc ccaaagctgc tgtagcacaa
    14041 accagccaag cagtatttac tactttcccc tttggactca tatctttcat cattttatat
    14101 actggaatac tattggctaa agtaaaaact aatcctgctg cactagtggc atccatacct
    14161 aattttccac ccactattgt aagtggttta ttaagaactt taactaataa atgtaatatt
    14221 ggaaaagtac ctaataacac gattgcaata cttccaatta cttgaatacc ttctcttata
    14281 ggtgtcatgc cttttataag tactatacct gttatttctt gaaatgctgc tgctcccaaa
    14341 cctatcgtta tcaatataga tataaatttt ccaaaaacta atgacccatt tatcataagc
    14401 gtagaattaa attttaaacc tactgccaat attactgcca atattatcac tggtatcata
    14461 tttataatta ccattgataa gttaaatcct gctattaatc ctcctataat acctccaaaa
    14521 ggtattgtta tcaagcctat cagtaaccct tttgcaaaga atggtctgtc ttcatattct
    14581 ataagaccta gtcctactgg aattgaaaat actaatgtac atccaagcat ggatgacaca
    14641 accaatcctg caagcattcc tgcctcttta ttgtgtgcaa gttccatagc caatggatac
    14701 cctcccatat cattcgctaa aatagttgca aacatagatg ggtcagctcc acagagattg
    14761 aagattggta ttattatagg acctagtaca tctgaaatta ctggagctaa acaaactata
    14821 ccaaccattc ctaaagccaa tggacccatt gaattaaacc cttcttcaaa ctgctcgcca
    14881 agtccaaatt tatttccaat tattctatct aacgctccta aaagcactcc cacagccatt
    14941 atgtataata taattttatc aaagggcatt tttaattcct cctatacatg taaaatactc
    15001 tattgtaact taattagcac acaccttttt ggtatcgttt tcccaattca tagctctttt
    15061 tactgcttgg ttccatccaa gtaatatttc ttctcttttt tgagtagtca tatttggaat
    15121 atattttttg cttattgtaa attcacttct aatttcatca atactgttcc agacaccaca
    15181 agaaagacct gccatatatc ctactccaag tactgtcgtc tcaggatttt ctgcaatttc
    15241 aattggtata cctaatatgt cggattgaaa ttgcattgta aagttatttt tagtaattcc
    15301 accatctact ctcaacattt ttattttttt ccctgaggct aagttcatag aatcaacaac
    15361 atctttgact tgaagtgcaa cagactcaag agtcgctctt actatatgct cttttttagt
    15421 acttcctgtt attccaacca ttgtacctct agcatattgg tcccaataag gtgctgctat
    15481 acctgcaaat gctggtacaa aatatacacc tccagtactt gatatggaat ttgccatgtc
    15541 atcagtttct gaataatttt ttataactcc aatgccatct ctaagccatt gtattgcagc
    15601 acctgctata tacactccac catcataagc atatgtaact ttatctccta ttttccaagc
    15661 aacagttgtc aaaataccat tacctgctat atttagttcc tcacctatat tcatatatag
    15721 gaaacatcct gtaccataag tatttttaac cattcctggt tcaaaacatc cattgccaaa
    15781 taaagctgct ggttggtcta ccattgaagc agtaattttt atagaaaccc cacaaaatgc
    15841 ctccttatct gtagttccaa aatcaccact cgtaggcatt atatctgcta aaatacttct
    15901 aggtactcct ataatgtcaa gcaactctct atcccaatcc atatctctta tattaaaaag
    15961 cattgttctt gctgctgtag atacatcagt taaaaaagat tttcctcctg tcattttcca
    16021 tattaaccaa ctatctaatg ttcctgcaag tacttctcca tttttaattt tttcttttac
    16081 accttttaca ttatctatta cccatttaat ttttgtacct gagaaatatg ggtctataat
    16141 taacccagtc ttatctatta tcttattttc taaacctggt atattcttta aattttccac
    16201 ataatcataa gttctcctgc actgccacac aatagcattg taaagtggtt tacctgtatt
    16261 tttatcccat aacattactg tttcaccttg attatctata ccaattcctt ttatatcact
    16321 ttcattgatt ccattttcaa aagcctgttg taatacatta ttactcacag tttttgtatt
    16381 attccaaatt tctatagggt catgttcaac atatccagat tgtggatata tttgagtatg
    16441 ttccatataa tcgctaaaaa caaactctga tttttcgttt atcaatactg ctcttgttcc
    16501 tgttgttcct tggtcaattg ctaatacata tttcacttta aatgtccccc tttattttca
    16561 gaattaataa attctttttt attttttgaa tatataatct attcaagtta tttatatttt
    16621 atatcatcat gtggatttat gcaatattca ttacaatatt ttttgttatt tttaattata
    16681 gtgattaaaa aagtattttt attgaaatat tattgcaata tttattattt tattctataa
    16741 tctagttaaa ataacacata atattttaat atttacacat aatcagtcat tcaaaaattt
    16801 aaatttaact ttttaaggag gctttacaat gaaaaaaaca tatctattca gtcctggacc
    16861 agtaatggtt acagataaag tcagaaattc acttcttcac tatgatatat gccatagagg
    16921 aaatgagttt atggatttat tcaaagatac acaacaaaaa atcaataaac tatataatgc
    16981 aacttctaat tactactcat taattgtatc tggctctgga acatctgtaa atgaatgtgt
    17041 actatcttct atctttgaca aagaagatgc agttctgcta gttagtaatg gtgtttttgg
    17101 agaaaggcta gaagaaatat tatctactta taatgtaaaa acatataaac ctaattttga
    17161 atgggcagaa tacccggacc tagacatttt agaaaaatac ataattgaaa attctgatat
    17221 aaaagtaatt gctatggtgt tccatgaaac tagttcagga atgataaatc cagtccctga
    17281 actaggaaaa cttgctaaga agtataacaa aatatttttt gtagatgcaa taagtgcctc
    17341 agcaggtgaa tatattgatg tggaagagtt taatatagat ataataacag gtgttggtgg
    17401 caaagctctt ggagcatttc caggttctgc atacctttgt gctaaagaga gtattttaca
    17461 aaatattaaa gaaaatcaat gtaagaatgt gtatcttaat ctttataaac actataaagt
    17521 tgctaaatca tcttctcaaa caccaaatac acctaatgtt actttaatat ttgcccttaa
    17581 tgaagcttta actgagtttt tggaagatga cagtaaaata gaaagataca aagaatgttc
    17641 tgctatttta aggaatggta tggaagaatt gggtctaaca tttttacttc cagataagta
    17701 tatgtctaat acagtaacat ctgtatttct acctaaagaa atcaatgtaa atgagtttat
    17761 attagggcta gaaagggaaa gtggctatgt tgtctatcca ggaaaaggta aattcctaga
    17821 tgctaatata ttccaagtag caaatatggg agaaatatat ccagaagatt gttataaatt
    17881 cttagatatc ttaaaatcaa aattggagat aaaaaaagtt atataacagt ttgtttgtaa
    17941 aatcctacaa tatatcagta taaattaaat agactgattt ttttaaattt tcctataata
    18001 aattatattt tatatttttg tataagggtg aactataata tgtaaagttc acccctattt
    18061 caaatctttt tccttcaata taatcttttt cttttaattt attttatatt tttgacataa
    18121 ttattcttac atttaattgt tttttatttt tgacgcaatc gttttttatt cagtcatttt
    18181 atattcttga tacaatcatt cttacactta attatttttt atttttgaca caatcgtttt
    18241 ttattcagtc atttaatatt ttcaacacaa tcattcttac attctattat tgtttgtttt
    18301 gatacaactg tttttattca gtcattttat attctcaaca caattacctt ttcattcaat
    18361 caaagtaatc tttaagcatt atttttcaag ctcatcaaag taattatata cgataacctc
    18421 acctgtctct ttatctgtag tctttataat gcccttagct ccatttacct cttcttttgt
    18481 aaattcatca tctaacacaa ctttgaattt atcttccttt ccataacctt gttgaaattc
    18541 atctctcaaa attgcatttt tttctgcttt tggcttcaac tcaaattcaa ttccagattt
    18601 catttcatca aaataattta atactacaac ctcattattc attttattag ttgtctttat
    18661 aatcccttta gctccattta cttcttcttt tgtaaattca tcatccaatg ttatattaaa
    18721 tttgtcttct tttccatatt cctcttgaaa atccatcttc atttcattgt atttagtttc
    18781 aaactcttta ctcttgttta attccatagc tgatacattt gtcaatgatg ctccaattgc
    18841 cattactgtt aatgccatta ctataccact tttaaatttt tttactttca tatcttacct
    18901 cctaaattaa atgttctcaa tgaaattata taaagaaaat cctaagtttt acagtttcat
    18961 tcttgtaact aaaattacta ttatgtaacc aaactttttg cttcaactat tccttaaaag
    19021 ttgctttttt gaggaatagt tgaagttaaa taattagatt ttatctatat ttatagattt
    19081 aaaatcaaat ttatccatat aaataaatac ttatttttgc aaaataaaaa tttttaatta
    19141 ctccaatcct tcttatttac ttaaaatcct atgataagaa atttaaaagt ctttaccttc
    19201 tttaacaata atttatttac ttttaacata caaaatatag agagtatatc aaaatcattt
    19261 tactgaatat tctaaaaata agtaccccct attagtatat atatttacat attaaaaaaa
    19321 agttttaaat aaagttatat tttttacaac agttttgtaa ttaaaaacat attgaaggta
    19381 aatttaaagg tactatatac tataaatatg tcgacacaat aaatttaaaa aggagaatta
    19441 aatatgaaaa aaagattaat aataatgata ttgtctgttg tcttagtact atcaagtata
    19501 cttacgatat ttgcatattc aaatattaaa tataataata ataaattgat atattctaat
    19561 atgatagata aaaaaactca aaatagtgta aaggaaatcc taaaagaaaa taaaattaat
    19621 gaaaaagaca tagatacatt tattaaagca gtcaacaatt ataataaatt gcaagttaaa
    19681 atacttcaaa ataatataaa tatatctaaa tctggatatt cttcaatcaa tgcaaaacaa
    19741 gttccatata acctagaaaa attgcaagat aattgggtga aaaaattccc tgattatatg
    19801 gatgtaaatt gtagaataac tgcttttaga ctttttaaag attttataaa ttcaaataag
    19861 aaatttactg gagactctat tgatttgaat gtcgacttgg acactataat gaataataaa
    19921 gatgctaaat ttagcatcaa agatgtagaa aaatttatca acttcttttc tgcaatacca
    19981 gctaaagata cagatgatac aatcaaaatt tcagaacaaa taaaaaatga atggaaaaaa
    20041 agaaaaatat cttttaaaaa taataaaaat atatctatta taaatggatt tcttcgttat
    20101 ccagaaacta aaaacgtatt tataggtcat acaggcatat gtattaaaac taaaaatggc
    20161 attctatttt tagaaaaata cggtgttaca tcaccttatc aagttactaa atttaaaaat
    20221 aaagaagatg taaaaaatta tatgtttaat agattgaaga tgtcagaagg tgaaattgaa
    20281 ttacctgacc ctataataat ggaaaacgat aaacttatga aataataact ttaaacgcaa
    20341 aataatattt ataattacta agtcaatata atagagaagg tctatagatt tgtgtaaata
    20401 caagcttatc tacaatctta tataagaata tttatataaa taagatagat taattctaag
    20461 tcctattatt ctattgaatt ttattattat tttatttaga ttctattgtt attttactta
    20521 gaacttatta ttattttgtt taagttctat tactattttc aatcatataa atttacaaaa
    20581 gactgtacat aaatttattt tatacaaact tatctacagt cttttgtgtg aatatatcta
    20641 aacaatatcg tatttattta tttcctctgc acagtgagaa tcagaaccta aaattaatgg
    20701 aatatcgtat ttttttacca actctaataa gtatccatct atataaggct ctttgcagta
    20761 ctcttttcta tttccagaaa cattataatc aagttcataa cctttttctt taattaactt
    20821 tattaactct tcaagaattg tatttccttt ataatcgtat ggaaatacct gattaaactt
    20881 tctaactaaa tttaaatgac ctattctttt tggtttatgt atacctaaat ctgaatttac
    20941 agcttttatc aatgtttcat aatacttatt ataaacagct tcaatacttc ctaatttgtc
    21001 gattaaattt tgaaactcct ctacactgta atctacacaa taataatcat catcaatctt
    21061 aattatatgt actgataaaa gagaatcctc taattccttt ccatatttat ttaaattctc
    21121 ctttatccct tcttcgtatc cttctataaa atccacttcc gacccaacat ttatcttgat
    21181 tttaccctca tacttttctt tcaacttctt gacatcatta aaatactttg gtaaatcttc
    21241 aatactcata caactatcat tttcaggagc agggtctcta aatccatttg catacggaaa
    21301 atgttctgta aatgtcattt ctgttattcc aacatccatt gctctttgta catatttttc
    21361 aaaatcatcc ttacttccat gaggacaata aggtgaatgt atatgaccat ctttcataaa
    21421 taattcactc cttttttaaa gaaaaaatct aatataaatg cataaatctt ttaacttcaa
    21481 aataatattt ttaatcatgc taatttttat tatatctaat attagagtaa ttttaaactg
    21541 ccttttaagt atcttatcat tagtactatt ttcaacttaa cattaatatt attttttaac
    21601 ttaacattaa tactatcttt aaatagtaat actcaatata taattactta acataaaata
    21661 actcaaattg cttgatgagg cttttgcagt atttaaagaa tatataatta tttaacatta
    21721 aataatccaa tcttgtaata aaatttactc attaaagtat attacagaaa atacctcaaa
    21781 ataaactttt tagttcatga tatactcttt tttgcatgaa attagatata tttttatcat
    21841 ttcaacaatg aaagggaggc ttatatccat ctttagacaa ccccttagat aaaattttta
    21901 ataattaaac atcacaaaat ataaaaatcg cttatatcaa acgtataagc gattaattat
    21961 atttacatat gttttttagc ctctaacaac attttattag cttcttccat atactctata
    22021 gccttttcaa tactctttgc tgtttcttct tttccaatag ctctagcctt ttcaacccac
    22081 tctctaaatc cttcttcatg agtttcattg tgatttatcc aatgtactaa aagtattttt
    22141 aaagtttttt catccttaga ctctgatgat tcctcatgac tatgttcgtg gtcgtggtcg
    22201 tgagcatggt catgtggatg ttcatgacat atatctccat gagtatgttc gtggcagtgg
    22261 tcatggtcat gatgtccatg atgatatctt acaatctctt tatcaaaaat cattagttat
    22321 tccccctgtt attttatatt aaactctttt tttataaggt ttacagaatc ttttacaagt
    22381 agtgctaatg gtttaccttc taatccaact aactccacat tttctggcat aataggtaaa
    22441 agtatcttaa gtgcctcact gtctgcaata gcttcactta tattacaagt aacttctccc
    22501 atcatagaat taggtattat tacagaaata ggtgcaacta ttatatttgc ttttttagct
    22561 gatactacta tagcattttc ccctgtagct cctttattag catgagcttt catcatagaa
    22621 ctagttgcta tagaattagt ccctagtgca taaatttcta catatgtagg taactcttct
    22681 ctcaaagaag aaactatctg ggctccaata cctccaccca ttccatctat cactgctatt
    22741 atcatatgaa attcccctat taattaattt tcttgtatta ttatcttatg gtccatcaat
    22801 cttatttctt taattatccc atctattatc ttttgctctc ctaataaatc cgttaaataa
    22861 atctttccat cacaagggtc tatattaacc acattttcca taaccttttc taactcatta
    22921 ttacttttac aaatgaaagc tgatgattca cacatataaa tctcctccta atatgatttt
    22981 tattaaaaat caaaaaaaga ttcccttagg tatttcaata caccaaagaa accttcatga
    23041 tttcagtttt tgtatattat tataccttaa gtatatcact ttaatttatg taaagtcaat
    23101 taaagttaac gctttttatt atactcttca ttcttgtatt tttcatatag ttctttaact
    23161 aattgcttct cataatcagt caaatcgccc tccacaaact caacattaaa ttgttcagct
    23221 aatcccttta ctatgttttt ctgtagaata tctatgtcta tttttctgcc aatttcattt
    23281 tctattccac ttgctttttt agctgtaaat ttcatagctc tttctttcaa ttctggtgtc
    23341 ttagttttta ttaatttaaa caatttttct acatcaaaat ccagtattat tgaaccatgt
    23401 tgtaataaaa caccatcttt tctgctttga gcacttccaa ttaccttctt attattaata
    23461 gtaacttcat atgatgcatg tgcattaaaa catgcagctg ataaattttc cctacttatt
    23521 ctctcgcctc tatttaaatt atctgtttct ataccactta gattcaaccc tttaactaga
    23581 ccttcactta tatatctata agataggttg atgctcttat cgattaaagg attatcttct
    23641 cctataatta tactataagt tagctcatta tcatgtaata cagctcttcc acctgtaatt
    23701 cttctagtat agtctatatt catacatcta cacttatcta aatctatctc atcttccaat
    23761 ttctgaaaat atcctatact caaacaagct ggttcccatg tataaaatct aagagttggc
    23821 ttattatgtc ccttcttata tgcagtaaat atagcttcgt caattgccat attcattgct
    23881 ccttcatatg atttattatg tataactctc cactgattca tgattattac gcccccattt
    23941 ttagtataaa tatatcctag aaatcctcta tagatttatt tctcttattg ctaaatctat
    24001 ctcactttga tttaaaggtc ttgaatagtt gtatatagta cttcctggtc tttcattata
    24061 gtatggtcta ttacaatcct tacaacctct tatttcaaaa ggatagcctt tgtttatgtc
    24121 ttttaaaata tcattgtcaa gctttatact atttaagtat ccatttttaa acttaaaata
    24181 ctcttttgga taacctttat ttatcatata agacattaac tgtacccttc tataactttc
    24241 tatgcttggt tgatttatct tttccatttt agttcctcta actggagtaa atgcaaataa
    24301 actaatcgtt acatcatttt cctttaaata agtatataaa ttgtatatat cttcatgact
    24361 ttcacccata ccaactatta tatgtgtact tattttattg ggatatgatt ttgacatttc
    24421 agttataaat tttaactttt catcatagtt atttcctttt attttttcat atagttcttt
    24481 atttgctgca tctattgcta ttcctatttt atcaactcct actgaaaaaa acttctttat
    24541 ctgttcatca ttttctaatt ttgcagatag tgaaattggc atgtctatct taccacttat
    24601 atagtttata aagtctaaaa cagagttatg tgcttcttca cttgccattg attgaataca
    24661 tattctcttt atattttttc ctttataagc ttttaaagca tctaatattt cttcttttga
    24721 aaactctggc caaattactc ttgataattt atcttttcta gtactacttt ctattgattg
    24781 agaacaaaaa ctgcatttat ttatgcattt ttctccaatc attatatatg ctgtggtagg
    24841 aggtatatcg ctttttttgt ttaatatccc aagttctatt gctgtcccaa ctgatagtct
    24901 aatcataaaa cgcaacactc cttacactca tttattgtca tattcatttc tatagcctta
    24961 ttctttgcag accttgatgg taatactatt ctattgactc cacacattat agataattgg
    25021 tctagctctt ttctatacac tcctcttggt ctcatacaac ctagattaat ttcagtatct
    25081 ggtaaattaa ttctagcttc acataaaata tctgctaccc cttctaactc aggaggttct
    25141 acattttcat actcagtccc ttttgttgga ataaggacta taaatgtaag cttattagga
    25201 ggattttttt gtaggtattc tataatttca tattcaccct ttatttgtcc tccttttaaa
    25261 ccaatacata tatgtggtgc tacttctgta tgttcttgta ttgtattata gacttcaata
    25321 taatcttctt tactcttttt catcttataa acttctctta ttgtctcatc atcaaacact
    25381 aaatcaaaag aaactatatc taaatactta cacaactcta caatgctatc tttatccata
    25441 agacctaaat gagcattcaa tctatatcct tgctctttta agtttttaat ggtattaata
    25501 tgagtattta tagggacatc tccctcataa ctacaaccgc cactcaatag aaagctagtt
    25561 atattccttg attgtacttt ttcctcatat tcttctattg gcaccatatt gtttaagtag
    25621 tgaccgttgc aatgggcaca tttcaaacta cattgattgc ttgtagtact aagagctagt
    25681 gtctggttag gataagcaaa ctctatttta tttccaaaat tctttaattt aatatcaaat
    25741 gctatttcca ttttttcaga tagattctta ttcatagttt attccttctt aagtacattt
    25801 tactcttcta gtaatttctt tgctgttggg aactgctcta tattcgtatg tggtaagaaa
    25861 catgcagaaa taaattcatc catataactt ggatatacac taagttcaac ataagttatt
    25921 tgattaccta tttcttcaag tttatttttt gcatctttac ttataagagc taagtaagaa
    25981 ccaacaagtg aactattccc tatataagta aatttttctc tatcaatatc aggaagtaac
    26041 cctataatta ttgagttttc tatgtctagg ttgtttccta taccacctgc tatataaacc
    26101 ctatctaata tactaaaatc catacctaaa ctatctacaa gagtgtatgc gcctgaatat
    26161 atagctcctt tagctctgat gaaattatct atatcaactt cattgactac tatatcattt
    26221 tctaaatcaa attcttcttt aaatgctaat acatactctc ctatctcatg ctcattaaat
    26281 ctaactctct tattatctaa atccctgtat attttacctc ttctatctat taccccttta
    26341 gtaatcattt gacagattaa atcaattata ccagaaccac atataccaac tggtgcacat
    26401 tcatcaataa tctttaatgt tggctctaaa gtttctttat caataattac tttctctatt
    26461 gctcctgctg atgctctcat tccacagctt atccctccac cttcaaaagc aggtcctgct
    26521 gaacatgcac agctcatcat ataatcttta tttccaaata caatctctcc attagttcct
    26581 aaatctataa ataaaacatt ttcttcactt gaccatatcc ctgctgataa tacacctgct
    26641 gttatatctc ctcctacata acttgcaaca gatggagcta agtacacata tgcactgtca
    26701 tttacaaaaa gaccaacatt ttcacccata agttttggag attttaaaaa tggtggtatg
    26761 taaggttctt gtcttaagaa gtctgtatac acacccaaga ataaacttgt catagttgta
    26821 ttcccagcag ccactaatgt aactacatct tccttatcta ttccatttct ttcatatatt
    26881 gtttttaata gtggatttat agtttcctca actatagctt tatgaagtgt ctctaatcca
    26941 tttttcttag ttgaatatat aattctattt ataacatctg ctccatattt tatctgtgca
    27001 tttccagaag atgctttatc tactacctct tttgagtata ggtctactaa acaaacaact
    27061 actgaagttg taccaatatc tattgcaact ccgtataagg aattttcttt atttccttgt
    27121 tctatattta tgattgtaag tttcttttgt ttttgaacat atgttattgt aaccttaaaa
    27181 tcactctttc taaatacagt tggcattttt ctaagtatat ccaatctaaa atctatttca
    27241 ttatatccta ggttatttct aacatatctt tccaatctat ctacatcact tatattatca
    27301 tctaaatttg gttcttccat ttctatgtat ttctttttta tatttgtttt aaattgaagg
    27361 ttatgttctt caattatttt tttagctctt tcaaaaattt ccctatcctc ttttttatta
    27421 ctaccttcta tcttcatacc atgcatagaa gatgatactt tagaaggaac ttcaatttct
    27481 atatctgata ttacctttgt acaacaagct agtatataac cttgctccca ctcatcatct
    27541 gttatatgtc ttgttttttc tgtatcaact tttccattca ataattttac tttacacttc
    27601 ccacaagaca cgtttccatt acaaggagca tctatgaata tatctgcatt tcttgcaacc
    27661 tctagtaata tatccccctc attacaataa acctctttat tgtttggtgt aaaacttact
    27721 tttatcatgt tatccctccc aatttgttct atatatgata aaaggaaagt atagctaaat
    27781 ttgcgtgcac tcaaatttta ctatactttc cccaattata ataatatctt aatctttaat
    27841 tattctaatc cagcctcgta ttctttatca cttaataacg tatcaagttc agccacatca
    27901 gctagtttta cttttaatat ccagttttca tatgggtctt catttataca ttctggctca
    27961 tcttctaatt tttcattaac ttccaatacc tcaccattaa caggagatat taaatcagaa
    28021 gctactttag aagactcaac tactccaaaa tctactccct tagttacagt atcttctact
    28081 tctggtgttt caacaaacaa tatttctcct aattgatctt gagcatagtc tgttatacct
    28141 atatatacaa catctccatc aattactttt acccactcat gatctttaga gtattttaat
    28201 tctggtaata atttcatttc gatagacccc caatattata ttattgatta aattacttaa
    28261 atcatattaa tattatatca tattatttta aattttccat caattacatt acactttcca
    28321 tagctaatgc tggatgacct ttttcttcta agaaagctaa tactccttca gaatcgtcag
    28381 ctatagtttc atcacaaacc atatcacaga agttttctat tccagtcatt tctttaactg
    28441 tagcatttaa tttatcagct acataatctt ttaattcttt tggcatccaa actattcttt
    28501 ctggtccacc ttctgcataa gcgaattttt tagaagatat gaaatgtctt ccgtgtccca
    28561 taaatcctgg agtttgaact cctccacctg tcatagaagc aagttctccg aaagtcatac
    28621 caactggagt aacacttgca aattctctgt taactacaac aaatccattt gcttctggca
    28681 ttataccaca gatacattcg aagcatccac aagaagtcat tgggtcttct agtatagagt
    28741 ataatgtaac agattcaact gcaccttgag atatttggtt tacagtttca tttactgaat
    28801 tccatacacc tgttctatca tctaagcact cgcctttttc tattggttga caaggacctg
    28861 ttgggtctaa ttctttagta gccttagcat ctaaccagct aactgctcca caaagaccaa
    28921 gtctttcagg agtaacaaca caaacgtgag ctggtgcaaa tgattgacat aagttacaag
    28981 aatagaaagt atcaacactt tcatcaacta atgaagctaa tctttcatct ctagcattgt
    29041 acttagctat tgcttctcct tttaattcag aaactttttc agcatctgta attatagtta
    29101 tttcacactt atcaactact gattcaaatt catctaacat cttagcatat aaaacttctc
    29161 ctatatgctc aagtctaaat cctttatcaa atgcatcttt agagattctt acccaagcca
    29221 tatctctttg tccaacgtgc attacacctt ctatatagtt taagaagtag tggaaacgtc
    29281 tttcaagaac tggctcgaaa tcttcttgca tatttttacc agctatttta actattacag
    29341 caagtggtaa tcttaataca ccatctgcat caacttcatc tatatttggt cctattattt
    29401 ctatcttatg gtcttcaact tctgttgctt ctttcttaac aacaagctcc caagcttcag
    29461 ttctgtttcc accaaactca gcaaacatat cgctctttct aattctctca ccttcaaatg
    29521 ctgctgcaaa tgaaactggg ataggtattt cagtaacttt tatttttatt cctctagctt
    29581 ctaatgaagt agctactatc ttatcgtaat ctttttgagt taataggttc attggaactt
    29641 caagaactgt ttggtcagtt ataactggga atcctaaagc tattgctcca gcacctgcag
    29701 atacaactaa ttcacttaat ggtccaaatg cattaacaaa tgcaggaact ctattagctg
    29761 tgtactctaa taatccgttt aaatcaccag gagttaatcc accgaatatt aatgctgctc
    29821 ttactgcaac agatacaacg tgtataacag aagttacatc atatcctaga ggtataactc
    29881 ttagctcaag acccattttt actccagctt ctatagcttg gtctataact ttaccaacta
    29941 agaaagttag taaaccttta gattggtaat cttttataac ttttgctgct gattctgcat
    30001 ctggacactc accaagaaca actgcaacac caggtatatc tcctgtaact aatggtacac
    30061 caagtgatct gattataggg tcagttatat gacctgcaca tggctcacta tatggagcat
    30121 ccatagttga atattttaat gcttcaatta cttctgctgc taaagctgta gctaaaccag
    30181 cattaaaagc atgttctagt aaatgagttc tatttattaa agattttact acttctaaag
    30241 caccttctaa gtcccctaaa gtgttcatct tttgtcctgt tgcagcatat atacaaggta
    30301 atgaatatgc tgtatcaggg aaagcaactt tatgctcttt tccattcttt tctatagctt
    30361 cagctaacat agcttgagcc gcacctaaag cttgttctga ccctgtaaag attatattat
    30421 atagattcat tctatttccc cctttaatat ttacacctat tttttattaa ctatacccta
    30481 aaaaccctag aaagcaaatc caccttcatt taactctaag aaagagtctg catataaaga
    30541 tgccccgttg attatgtcac aagattttac agttttcatt aattctgggt cacatggatt
    30601 cattatagct gaactaaatc cattagccat tgccattgct aagaatgcac tatctaaaac
    30661 aggtctaaca tgcttaggac atccattaga tacgttagat aatcctcctg tagttaaaag
    30721 tcccatctca gtcatcattt ttatagcttc taaaacttct acttgttttt cttgcatacc
    30781 ttttataact aagcataatg ggtcaaatag tatatcagtt ggctccattc ctaacattaa
    30841 gcctttttct aatatttctt ggcagtaagc catacgctca tcattatctc ttgggatacc
    30901 ttcttttgca cataaaccta taaccattgc ttcgtattca gctgctatat ctattaaatc
    30961 aaatcttgaa ccagcatcag cagagtttat tattggtttt gcattagttc tatcataaac
    31021 tttaagtcca gcttcgatag cttttttatt agctgtatct aatgctatag gaacattgtt
    31081 gaactcagat tgaagtaatt tgattcccca tgtcattatt tcttcgccat ctctttcggc
    31141 aggtcctata ttaaaatcta tatagtgtgc tcctgcttct aattgctctt ttgctctttt
    31201 taagattgga gctgggtctc tttctgctaa tgcttttctt attgaaggtg atatacaatg
    31261 tattctctca cctataatca taaatttttc catttataat gtcccccttt aaatttaata
    31321 ttaatagtta gttaatattt tattagtttg tttggcatgc tttttcttgg tatcctttta
    31381 agaacttagg taattccata gattcctctg gtcctataac tacattccat cctggtaagt
    31441 tatcttctat atcaccttta agtactgcaa cttttcctgg aatgataagg tctttacaat
    31501 tagttacttc ttctacctta ctttctttaa tgaaagcaga gattgagttt ccaccaaatt
    31561 taccagcagc ccaagatgta agaactgaat atccacctgc atctggtatt actaaccata
    31621 ctggaacttt tgatctttct atatcaccag caactatgaa gtaagttaat gcaaagtcaa
    31681 cagtaactaa tactggagaa ttttcatctg ggttgttgat tggataaatc tttggttcaa
    31741 ctctcattgg tctttgtggg tcagtgtata tgttttgtct tagtgcaaat aatggtaatg
    31801 ccttagcata actaatatca tctattacta ttatagaacc gtattttata gtgaatattg
    31861 atgataaagc aacttccatc atagggtttg aattagataa tctatttgca aatactattg
    31921 aaggatatcc aaatgttcta tcttgctctt ttaaagctgt tcttctaact tgtatagcat
    31981 tagtgtaagt atcttttatg ttttctcctg ttacatctaa tactaattct ttatatccag
    32041 cagcttgaat taattcaact gtttcatata attcttctaa gctaccagct tttacaccta
    32101 atggtaaact tgctccttta actacttcta tcatatcttt gtagttctct ttagttgctc
    32161 catataccat tggtctagca tcttttaata cttcaacagc ttctttaact acttgtgcat
    32221 catcacaagc taaaatataa gctacttcta atccactgcc ttttatttta tctattaatt
    32281 ttaaatacgc atctttatct ccacaatact ctaaaacagc catttcaact ttcatttgtt
    32341 caccaattct tacatagtca acttttttca tgttagctat tttagcatct actgcttcat
    32401 cactcataca agtacagaat gaaactgcat atctatttct acttactaat gttttttcat
    32461 gtctaaataa tactgtttct ccacctagtt catactcaga tgcaccagca ccaactttta
    32521 aagctttcat taaaggtgca gtagcctctg ataattttgc tatagcgtca tcagacatat
    32581 gtgggcattt acctacttct acagctcctg aagcaacctt catagagaaa gccatacaag
    32641 ttgggaatcc acaatcctta cagtttttct ttggtgttaa tttaaatata tctaaagctt
    32701 ttaatgccat tattttttcc ccctaacctt aacaatttaa ttaagctaat gcgttaacca
    32761 attcttttat agtttctatt gattttggat gacgaagtat aactgcgttt gaaccaccta
    32821 ctaaaacact tgctgcagtt gaaacttcca ttgctatagt tctttcttct ggacaacccc
    32881 aatctggctc atcttctatt ggagctatag cttctttaac atgacctact tcaaaagcta
    32941 ctggtgttat gataggcatt tgtaatgtct tatcattttg accaaatgct gcaagtctaa
    33001 ttctatccat agtagatgca acgtactcat atccataacc aactgctgaa catccaacat
    33061 tcataactat attttcacct ttaacgccta gttgagttaa caatacgttt aattgtttag
    33121 ctaggtttat atcaacagaa gattcagctc ctactttatg tgcataagcc ataccagctg
    33181 atgctcctac tcctttgtag ttatcttcta ctgctgacat aaataagcag ttatgtccat
    33241 ccaatgtttg agctaatttt tcaaataatt taccatcttt ttcatgattt cctgaaccag
    33301 ctacaactaa aggtaatttt attgcttcaa ttactgcctt agctacatca gcacattcat
    33361 caactgactt atctaatcca tttgggtcag aaccatcaaa ttttaaacaa ataaaatctg
    33421 cttgtgtatt agcttcaaca tatttagccc actctactgg acaattagct acatctttgt
    33481 ataattcctt atatgaatca gtccagcttt caggataaac atctgatatt tgtatcccta
    33541 tttttggaga attacctact tctccatcaa aactataaaa aggtaataca ttttccccac
    33601 ctaatttaat tgctttttcc cctattccta cttcaacttc tgatattttt ccagaatatt
    33661 tttgagtaga cattttaaat gccatatttt tttccccctt aagcctattt taattctaat
    33721 ttagcaaaaa tttctttcat agccacttta gattttgaat cttcaggtaa attaactaaa
    33781 ggaatacctg tagaatcata ttcatatatc aattcatcca taggtacaac accgattagc
    33841 tctaggttat gcttttcaat ttcttcttta acaccatcat tcataacccc atttggaact
    33901 ttatttacaa tcaaaagtat tcttccaaca cttaacttta gttcctcagc taaatctctt
    33961 attctagcaa cagcttgtat gctacgtctt gagcaatcac taaccaataa aagtgtatca
    34021 acatgtctag tgacttttct acttagatgt tccataccag cttcgttgtc catgactaag
    34081 tatttgtaat gaccagatat tttgtttact tgttctctaa gtatcccatt tacaaaacag
    34141 taacatcctt caccttctga cctacccatt actaataagt catatccttc gccttcttcg
    34201 attatagaat ttaatctaaa ttgaagatat tgtgcctttg tcataccacc agggaatgca
    34261 ttgcctaact tttctctttg atttacttct tctcttattt ctcctattgt tgcttcaact
    34321 tcgataccta atacttcatt tatgttagca ttggcatcag catctacaac taatactggt
    34381 ccctttttgt ccttaaccaa ataatcaatc aaaagccctg tgagacttgt tttaccagtt
    34441 ccaccttttc ctgcaacagc tatattgtat cccattttaa gacttccccc ttaaatctag
    34501 ccacagagcc ttatgtactc tgtctgtcta ttataattta ggtacagaat gaacacattc
    34561 tccatgtaca tcatgaagtg cttccattag cccttcagat aaacttggat gtggatgtat
    34621 tgcatcccct acttgttcta ctgttaatcc tagatgaaca gcaagtgaca actctgtcaa
    34681 taaatctgta gcatgaggtc ctactactgc tgcacctatt atcttatcag tttctttgtc
    34741 agctataact tttacaaatc cttggaaatg tcctatagct tgtgcttttc caagacctct
    34801 aaaatcaaat tgacctacat tgtattctac accttcagct tcaagttgct tttcagtctt
    34861 accaacacca gcaacttcag gttcagtata aacacatctt ggaattgctc tataatctac
    34921 taccttagtc ttacctaaag cattttcaac tgctacgata ccttctttag aagcaacatg
    34981 tgctaggaaa ggagtatcta ttatatcgcc tattgcgtat ataccttcta cattagtttc
    35041 aagatgttca ttaactacta cttttcctct ttccatttca attcctatat cttctactcc
    35101 agaattgtca aggttaggtc ttctaccaac acatactaat gcatattgtg cttctataac
    35161 ctttccatta gaaagagttg caacagcctt accatcaact acttcacaag tttgtactcc
    35221 aatacctgta ataaccttaa ttttatcttt cttaaattgt ctaagtagtt gtttagctac
    35281 atctttgtct tcatttaata gtatttggtc aaccatctca actatagtta cttctgttcc
    35341 caaggctcta aagaactgcc ctatctcaca tcctataact ccaccaccta ctattaacat
    35401 agactcaggt atctcttcaa gtccaagaac ttcatcacta gttattacaa cttttccatc
    35461 atatgggaac attcttggta caactggaac agaaccattt gctagtatta tcttatctgc
    35521 ttttatagtt tctactgttc catcatcttt agttacttct atagtgtttt tatcaattaa
    35581 ttttccaaaa ccattaacta agtttactcc tcttttttca aataagaact ctataccact
    35641 tattaattgg tttacaactt tattttttct ttccattatg gcagtgaagt ttggcttaac
    35701 tgtaccatct atttctatac caaaatcctt tgcttctttt acagtattca atactcctga
    35761 agaagcaaga agtgctttag ttggtataca tcctgcattt aagcaagttc ctccaactct
    35821 tcttttttca acaactgtta catctgcacc aagcatagaa gcttttatag ctgctacata
    35881 tcctcctggt ccaccaccta ctactactat cttcatcttt aattccccca aacctaatat
    35941 aatctacaat cttgcaatac tataaacctg cggcatccag tatagcaggt atattctctt
    36001 ctgccccaat tgccattata tgaacaccat cacataaatc ttcttctttt aattgtttga
    36061 taagttcccc tgccattttt attccttcac ttacccagtt ttcttttcca gctgctctta
    36121 atctttcgat ttgttcatct ggaacaaata ttcctggtac atttgcagtc ataaatttag
    36181 ccattcctgg tgattttaaa ggtactattc ctgctaatac cttgcaatcc atatcccttg
    36241 ttaattctct aaactttctc attgtattta tgtcataaag tgcttgtgtt tggaaaaatt
    36301 tagcccctgc ttttattttc tttttcattt ttaataattg aacttctatt ggagaatatt
    36361 ctggagttac tgatgctccc agataaaaat ctggagaacc ttttaactta ttaccagcca
    36421 tgtccgtacc tgccattaaa gtctcagcag tttgaagtat ccccacagaa tctaaatcaa
    36481 acactccctt agcttgtgga tggtctccta cgctagtatg gtctcctgtt agtgccaaca
    36541 gattgtttat cccaaataca cctgctgaca acatttctcc ttgtatagct attctatttc
    36601 tatctctccc tgttatttga ataactggct ctaatccagc atcttttaat aatttacatg
    36661 tagcaagaga agttgctctc ataactgccg attgaaagtc tgttacattt gctgcatgaa
    36721 ctcttccaac taacggcttt gcacattcta ttaaatgtga aaggtcagtc ccttttggag
    36781 gagccatttc agtagtaact gcaaattttc cactttctaa tgtttctctt aataagctca
    36841 tctctaatcc cccttttaac cttctaaaaa aaacttttat gaatttgctg ctgcttcttt
    36901 ctttttcttt gcacttaaac ttcttggatt attttgctta gaatagtctt ttggtggtct
    36961 tatttctgct aagttatcta attgtcctat agcttctagt ctttcatata tctttatcca
    37021 agcacaatca ttctctgagt ttacttcaca cttaccattt ttagctccac cacaagcccc
    37081 attcataaga cctttagcac acatagttac tggacatatc ccacctgtcc aaccaagttc
    37141 acagtctcca caagccttac aggcttcttc atattctcca actctttgta cttcccctat
    37201 aaacaaagta ttgttggctg gataaactgg cttatctttt aaattcttaa ctattgtctg
    37261 tgtaccatct ccacatgcta aagataaaac tgcatcagct tccttagtct cttcctttag
    37321 agcctttaaa tcctttttag actttaaaag gttacaagca gggtcaagca ttacatatcc
    37381 taagattttt ttgccttcac cttcaagagt ttccttcatt tttaaaactt cttcttcccc
    37441 accactttta caagttgctg cacattgatt acaaccaact aaaaccagct tgtcaaaatc
    37501 ttttaagtac cctagcactt cttctaatgg tttattttca gaaattatca tatctacttc
    37561 ccccctaaac cttccacata tatatatcta tttattgttt tgtaatttac aagccttaac
    37621 tacatgtttt gcaagtatag atgttgtaac tgaaccaacc cccgctggaa ctggagttat
    37681 cattgatact ttgtctaaaa ctgcatttgt atcaacatct ccacataaat ttccttcttc
    37741 atcaacattt ataccaacgt ctataactac cgctccatct ttaacaaaac tttcatcaac
    37801 catcttagct ctaccaatag ctgctattag cacgtctgcc tctgctgcta cacctgatag
    37861 atttttagtt tttgaatgac atattgtaac agttgcatgt tcacttaata aaagcattga
    37921 tactggcttt ccaactacca ttgatcttcc aagaactgtt accttacttc cttttaaatc
    37981 aacattatag tgttttaata tctcaactac tgctgtaggt gtacatggtg gaaatccact
    38041 tttatcccct tccattacct tagcagagtt tattggacta aaacaatcta cgtctttttc
    38101 cggtgcaata acatatttta taacttcttc atttaagtgt tttggaagag gtctaaaaca
    38161 taatatacca ttaacatttt tgtcatcatt aactctcttt aatacatcaa tatattcctc
    38221 ttgtgttata tcctcagcta actctaacac ttctgttgtt atacctatgt tttgacatct
    38281 ctttagtgct cctctttcat aagataaatc atcacttctt gccccaactc taactattgt
    38341 tagtttagga tttatacctt ctttaattag taaatcgact tcttttatta attcttcact
    38401 aatcttatca gctacaggtt ttccttttat aatttgtcct ttagttgaca ttccttccat
    38461 acattcacaa ctcccctaca agcaaactta aattaattta ttctatatac cacatattaa
    38521 taacatagta tctactttcg tcatttaaaa attattttcc aagagctttt tcaactttgg
    38581 cataaacttc atcacaaatt tttacaccat cttcaactaa cttatctact tctgttttta
    38641 ccttattaac atattcttta tcttgtatag aatttatatt tataactaca tttaattgtc
    38701 caccaagtat agcagctctt aaacactgaa ctcctacacc aacatcactt atagcaagtc
    38761 ttgaaccttt atcaactaaa tcttcatgta acttaatagc ttcatagcaa actcttacta
    38821 tgtttaatgg tacttcacaa gcaactttta atgccttttc cattgtttct gcttttattc
    38881 ttttttcttc ttctgtttct ttaggaagcc cgtaagcttt tgataatggt aagaaacatt
    38941 ctgcatcatc atctatcatt tttaataaat ctttttctaa ctttcctgcc ttacttaata
    39001 tctcttttac actttcttca tactcagcat acttcttctt ccctattgta aggttacata
    39061 ccatactacc taaagccata cctattgcac ctactagtgc agcagctccc ccaccacctg
    39121 gtactgcttc tttggaagat agtacttcta caaaatcaac acaagtttta tctgctattt
    39181 tcatgataat cctccttgaa aggatgtagg aaaatatctc aaattgttcg agatatttta
    39241 cgaactattt taagatatct cctactcttt atttataaat taatttcctt agaataatcc
    39301 agctattact ccattttcat caacatctat tttttctgct gctggaactt ttggtaatcc
    39361 tggcatcttc attatttctc cagtaagtgc aactataaat ccagctcctg ctgaaattgt
    39421 tgcttgtctt acagttattc taaatcctgt tggtctacct aatttagttt ggtcatcagt
    39481 taaagaatat tgagttttag ccatacatac tggaacttta gtaaatccaa gtttttctaa
    39541 gttagctatt tccttatcag cttcaggagt ataatctaca ccatctgctc cataaatctt
    39601 agtagctata gcatttatct tgtcttttat agataaatca tcttcatagc agaatctaaa
    39661 gttattttct tcttcatcca ataatcttaa aacttcttta gctactgcaa ttccaccttc
    39721 tccacctttt gcccatactt cagaaagagc aacattaaca cctaattcct tacacttgtc
    39781 ttcaacaagt ttaagttcag cttcagtatc aagtgggaat ctattgattg caacaactgc
    39841 tggtaatcca tatacttgag ttatgttttc aacatgtttt aataagtttg gaagtccttt
    39901 ttcaagagct tctaggtttt cgttatttaa gtctgcttta gcaactccac cattgtattt
    39961 taatgctctt acagtagcaa ctattataac agcatctggt tttaagtttg ccattctaca
    40021 ttttatatct aagaattttt ctgctccaag gtcagcaccg aaaccagcct cagttattac
    40081 ataatctgca aagtgcatag ccattcttgt agctataaca gagttacatc catgagctat
    40141 atttgcgaat ggtccaccgt gtacaaatga tggtgttcct tcaagagttt gaactaggtt
    40201 tggttttaaa gcatctttta ataatgcagc catagcacca ttagctttta attgtctagc
    40261 tgttacaggt tcaccttcgt agctgtatcc aactattatg tttcctaatc tttcttttaa
    40321 atcagatata tcacttgcca aacagaacgc tgccattatt tcagaagcaa cagttatatc
    40381 gaatccatct tgtctaactg ctccatcacc ttttttaccc ataccatcaa cgatatttct
    40441 aagttgtcta tcgttcatgt ctacacatct tctccaagtt attttcttag ggtctattct
    40501 aagtgcattt ccttggtgaa tatggttatc aagcatagca gctaaaagat tgttagcagc
    40561 tcctatagcg tgaaaatctc ctgtgaagtg taagtttata tcttccatag gaacaacttg
    40621 tgcatatcct ccaccagctg ctccaccttt aacaccaaat actggtccta gagatggttc
    40681 tcttaaagca actaatacat ttttgtctag tttagcaaat gcatcagcaa cacctatagt
    40741 agtagtagtt tttccttcac ctgctggagt tgggtttata gctgtagtta gtattaactt
    40801 agctttttta ccagtctctc tttttagtag attgtagtca acttttgctt tgtatttacc
    40861 ataaagttct acatcatctt cacctagtcc taattttttt gcaacctctc taatatcttg
    40921 aggcttagct tcttgagcta tttcaatgtc agatttaaat cccataactg aatctcctcc
    40981 cacaacctaa atagatttaa aatataattt ttgtaaaaat tgtattaata tgtaaaaatt
    41041 atattttata agataattat acatgtaaaa ttacttaaaa actagctact aagctattaa
    41101 aatattcaaa cttaatagct agttatttat ctaattactt acttattacc tatctattgc
    41161 ctatgtctaa cctttaacca acttaaaatc ccaaatcaaa atttctatct attttactta
    41221 tccttaagtg acattaattt atcttttatt gctttaatct gttctttcgt tgtttcacta
    41281 gaatcatatg gagattgatt agctctatca gaatcaataa catcttggtt ataataaata
    41341 aatcctaaag attctccatc ttctaagttt tggattataa attcttcatc ttccttattt
    41401 ctaattttat ttcctactac aaatacttta tttacaccta tatcatgtcc aagtttctta
    41461 acttttctat aagtctgtag acttctctct cctggttcta caactactat aaaagcatct
    41521 acaccttggg ctgttcctct tcctaagtgt tcaattcccg cttccatatc cataaccact
    41581 acatctttat tttgtagtat taaatgtgag cagagtctct tcaaaagaac atgctctgga
    41641 catacacatc cagtaccacc agaatctacg gttcccaatg taagtaatct tactccattg
    41701 tattctttac agaaattttc agggatatcg tctactttag gattcatctt aaacattttt
    41761 ccaaatgaac ctacagaagc agctgttcta tctgaaacta attttttcat ttctgatata
    41821 ggtactattg attcatatac ttcttttgga aaacctagtg ctaaagctaa attagcatca
    41881 gggtcggcat caacagcaac aactctgtat ccgtcttcag caaacatcct agagagcatt
    41941 gaagaaaaag tcgttttacc tactccacct ttacctgtta ttgctatctt cataatccct
    42001 accacctttt attactattt gctttttagt agttaatttt acagttaaat attttttata
    42061 ctctatattt ttatacacta aaaataaact acagctagtc cttacaggaa ccaattaaat
    42121 ttaccctact agcttttcaa agcagcatgt atagttttat tattctttaa tattgtttat
    42181 attttattta ttcagtatat tcattatatt ttgatgttct ttactttaat atttgacttt
    42241 attcaacttt attcaaccag aaacttctta taaaaacgtt tattctataa cttcactatt
    42301 ttttaagctt cagccttttc tgccattttt tcttcaacta atgcttcaaa gtgaacacgt
    42361 tttttctcta tatcttctat tattgtagct gctaactctt ttggatttgt atttacagta
    42421 aatgtagctc caactttatc ttttaattca ctagttaata tatccactgc tcttgaagaa
    42481 ccagctattg gaggcattat tcctaaataa gtgtctatac cagaagctac aacatagcat
    42541 cctatagcaa ctgctttttc tgacatccac tctggagcta tacctacaac tggaaggtca
    42601 cacatatcca tatctaagta attagctgct gctccaacta aatctagtat acggcttata
    42661 tcaacacaag aacccatatg tagtactggt gggattccta ctaatttaca gacagttgca
    42721 agacctttac ctgcatattt ttcagccgca tcagattcca ttaaaccaaa cttagctgct
    42781 gctgaagctc cacaacctgt agtaactact attatgtcgt ttttgattaa ttctttcatt
    42841 attgttacgt gagcctcatt tgaaactcct ttagcattgt tacatccaac tacaccagct
    42901 gctcctctta atactccagc ttttaaacag tctgttaatg gcttaactgt tcctgctggg
    42961 tctatatgag agtttacaac tctgtctaat tgatttacta ctgcttctac actataccca
    43021 actgttgcgc ctgattttaa ttcaggtata agtacttttg atttatctct atttttaaag
    43081 tttaaaatag cttctttaac tattgcttta gcatcatcaa gtgcttgttc ttctctaaat
    43141 tcaatataag ttgaacctgt tatcttagct tttggagaag ttgttacaaa tttagtatgg
    43201 taacaatctg caactctagc taatgcaggg aatatacatt gaacgtcaac tattacagct
    43261 tcaactgctc cagttattat tgcaagttct tgttgatgga agtctcctgc tatttttaca
    43321 ccatgtctca ttgtaatctc attacctgta caacacatac ctgctaagtt tattccatca
    43381 gcaccaactt ctttagctaa agctactaag tctggctctt ctgatgctaa aactatcatt
    43441 tctgataatg ctggctcatg tccatgtaat attatgttaa ctttattttc ttctaatact
    43501 gctaaattcg cctctgttct tcttggcact ggagttccaa ataagatatc gcttagacga
    43561 gtacctatca tagaaccacc ccaaccatca gccatagaag ttctaagact catgtgtatt
    43621 aaactatcta tatcaccagt acatcctata tgagtagagt gcattattgt tgcaatttct
    43681 ctatctatag ctcttggctc tatagcgtat tctttccata ttttttgtct tggttctggt
    43741 gcatttttta taaatcttaa tgtcccaaaa ggttttccaa attccattaa tgcaatttct
    43801 gccacttcat gtgctacatc atagatatct ctaccttctg tctctacatc ccattcttta
    43861 gctaaagtaa ttaacttagc ttcgtctctt attttatagt ttccatttgg atcagctaat
    43921 gctaatgtat gtgctatatc tctagcatgg tctgaatggg cagctgtccc accagcaacc
    43981 attcttgcaa agtttctacc tactatagta tgctcatctg ctccacataa cccccttgga
    44041 gtcttagggc ttattctaca tggtcccatt ccacatattc tacagcaaac accttgaaga
    44101 ccaaaaccac attgtgcctt aaagccttct tttctatcat acattgtttc tacgccttct
    44161 tctctagctt tagctatcat cttctggctg tttaaatcaa ttgttaacat tttttcatcc
    44221 atattcctat cccctttttt tgatttttaa aatcccccag gtgactgact tcatgtcata
    44281 aaataataaa ataaatgctt tctaagttga ttatatacta attttcagtg ctttgcttta
    44341 gatttaaata tttttttaac aaaattagat aatacataaa aattttattt tctacactta
    44401 atttaagttg attttatcgc ttgtttttgg ctatttgtta atatctaaac ctactttttt
    44461 gatacttcag tttaattaaa ttatgttttg atttttttaa atcaaatttg aaatttttct
    44521 agtcattttt atattattgc agattgtttg tgtatttatt atctttcact actgtttgga
    44581 cttattacag atttttactc cctaaactta attagtaaaa atttcttaga atagcatttt
    44641 ttggactaat ttttttgaat ttttttattt ttcctacttt aatattaatt atagcttcaa
    44701 attgaacatt tgtatatatt catttgaagc tataattatt ttttatataa aactttttta
    44761 atacagcatt aatttagaca ttttgactat tttttctact ttaattcatt tatttcaagc
    44821 gatttatcct atttcatatt tttagctaca atagccccat atgcaataga atataattta
    44881 gattcatgtg aatcagctct tgacactaaa actataggag cttttgcacc cataattata
    44941 cctgctgatt tagagtctgc catatatgta aaggctttcc ctattccatt accaacctct
    45001 attgttggaa ccaatatgat atctacatct ccagatactt cacttttaaa cccttttatc
    45061 tcactagctt cttttgaaac agctaaatca aaagccagag gaccttctac aattacattt
    45121 tcaccaaatt ctccttcttt acaagctttt gctaacatat ctgcatctac agttgcttgc
    45181 atcttaggat ttactttctc tttagctgcc aaacatgcaa cttttacagt ttccattcct
    45241 aaagcttttg cagcttttat agaatttttt aatatctttg ccttttgttc ataatcagga
    45301 gctatattca tacctccatc tgtagttata agtaatttat ggtatttttc taactcatat
    45361 accataacat gacttaataa actatcagtt ctaagaccat agtctttatt taacacttct
    45421 tttaatatga cagatgtatc taataatcct ttcattacaa agtcagcttc tttacttgat
    45481 actaatttaa ctgctatttc tgcacactct ttgatatcat ccttatccac tattttgata
    45541 ccacttaaat caaatcctat ttcttttgat atttctctta ttttgccttc atttccaacc
    45601 aatataggag taattatatt tctttctaca gcatccttta ttgccattaa aacttcttta
    45661 tcatgagctg ctgctacaga gagtacaatt ttctctcctc ccttaagttg ttctaaaata
    45721 tcatctaatt tttttatcaa aagtaacccc tcccttatat atatttaact aaataatacc
    45781 atatttgcta taccttttct caatttattt tttatgttat aaagaattta tataactaaa
    45841 agacagttta atattttatt ctgggatttc atcaatagct cttaaaagct ttgcaggaac
    45901 accacctaca atagtatttt ctgcaacatt ttttgttaca actgctccag cagcgataat
    45961 tgcattatct ccaatagtaa ctcctggaac tacagttaca ttagcaccaa tccatacatt
    46021 ttttcctatt gtgattggag aaggataagt tgtaccacgt ttctctggtg caataccatg
    46081 gttaagtgta gccaatacca cattatgccc aatttgcgta ttatctccaa tctcaatccc
    46141 cccctggtct tgaaaatggc aactgctatt tataaaaaca ttttttccta ttgtgatgtt
    46201 ttttccacaa tctgtataaa atggtggaaa cataaaaaat gattcatcta ccttttttcc
    46261 aattaactca gaaaataaac tacggacttc ttctggttca tgataagaag aatttaactt
    46321 acttgtgatt ttcattgcct cattactcaa cttaaccata aattcatgta tttctgattt
    46381 actagaaaca ggttttcttt gattgatatg tgttaaaaat tcattgatat tcattttata
    46441 catcctttca atatgtgctt taatttattt gtacattcta aataatattt taataaaaat
    46501 taatttaatt tatttttatt aaaatatcat tgcttctttt agtgtaatat acaattatat
    46561 ctatatcaaa tatctatatt aaatacttaa ctatacttaa aaagcatata aattgatata
    46621 attatataat aaataagtta atattgagga gggataatta tggaaataag agtattaaga
    46681 tattttttag caattgcaag agaagaaagt atatctggtg cggcaacctt tttacatata
    46741 acacaaccaa cactttctag acaaattatg gaacttgaag aagagttggg agttaaatta
    46801 ttggttcgag gaaatagaaa aatcacctta acaaatgaag gtaggctttt acataaaaga
    46861 gcagaggaaa ttatatcatt agttgataag gttgaattag aatttgacaa tcccaatgag
    46921 attattagtg gtgatatata tataggaagt ggtgaaactg aagctatgcg acttatagct
    46981 agaactgccc atcattttca tcaaaaatac cccaatgtaa gatatcacct ttttagtggg
    47041 aatgctgatg atgtgactga aaaattagac aagggactgc ttgattttgg tattttaatt
    47101 gaacctgcaa atatacagaa gtatgattat gcacaaattc ctgctactga tgtatggggt
    47161 ttactaatgc gtaaagatag tgcccttgca tcacaaagca caataaatcc aaaagattta
    47221 tgcaatatac ctcttattat ttcaagacag acacttgttg ataaagcaat ttctaaatgg
    47281 cttcaaggca gtaatgaaaa actaaacatt attgcaacat acaatttagt ttataacgct
    47341 tctcttttgg tagaagaagg tctgggctac gctctgtgtt tagatagact cgtcaatact
    47401 acagaaaaca gtaatctttg ttttagacct ctatctcctc ctttagaagc taaattaaat
    47461 attatatgga aaaaacatca aatattttca aaggcagcag aaaaattttt acttcaaata
    47521 caaaaagatt tttcttctat catgttggat acatctaagt tggatacacc taaagaaaaa
    47581 taaatcataa taaaaagtga gagtgtctca aaatgaactt tgcactgtat aaggcactct
    47641 tttttataca aagtaaaata tatgttcatc atttcagcac taaaaaagag gcttatctct
    47701 attttgagat agccccactt aaccaataaa attaaataca acttaacaat atccttactt
    47761 atttattcca agttctctta gacaagcaat cgtaatttca tcttgtggat ttaattcata
    47821 agctctttct atatagtaag ttgcatcatc aatatcacca ttatttaaat aagccattcc
    47881 taaattgcaa agtatttctg ggtcttcctt tatcttagcc gcttgttcaa aatactctat
    47941 tgctttatct aaattattct tataagcctc acaaagcccc aattccacta tagtatccac
    48001 ctgattaggc tttattatca atatcttctc aagatacatc ttagcctgct ctatttcgcc
    48061 cataccctta tatccaagtg ctatcataaa cagcaagttc caccaatcca tatgttcttc
    48121 ttctaatgga agtaacttct ctaatccttc cttaaactta ccttggaata caagtgtata
    48181 tccttcttca tattgaactt taaagtccat cttgcccaga ttctcttgta cctcagctac
    48241 taaatcagca tctaacccta gccttaacgc ttcttcccaa gtcagcttag tctttaaata
    48301 ttgaccttga ttataataat ggtagcctaa gtgataataa cctaatgcaa aattctcatc
    48361 aacatcaatt accttttcaa gcttagccat ggcttcaagt aagaatgcat tcattgcctt
    48421 atcatccata tctttttgat actgtttagc tatctcttgg caaacaatcg catagttata
    48481 cattgcatct aaatcatctg gatacattgt tataaatgct tttatatata ttaaagaatc
    48541 tgtatgttca ccaatctcaa agtattttct cgacatatat cccatatacg atttcaaatc
    48601 caaatcaata tcttttgata aagcatttaa aaacttctca tactctgcat tgtttttaaa
    48661 cttactatct attccaatta tatatatcat tgcatctgct atagacattg aatttaactt
    48721 atcttgagca gtcttttctt ttatcccttt tactaatacc tcatttttta taggtacatc
    48781 taatccacct tttggcactt catagctttt taatttaaac tctccaccat gttttatagt
    48841 tataaaagct aattcttctg ctttttttag caaatatttt tctatcttaa aatccatttt
    48901 atcctcctaa atacttaaat ctctacttta tgagttttag atttctcaaa tattttttta
    48961 tcatcatact caaataagac tacattttct acttctctta tttctgtatt tatatcattt
    49021 tttaaatcca caacattaaa tctaaatggc tcaaaatgaa catattttat tatatgttta
    49081 gctggtttat caacaaaact aggtaaatat gttgataagt tatcattatc ttgtaaaaat
    49141 acatggcatc tattcttaaa atcatccaca tctagcttat taaaaaactg tggtatattt
    49201 gatgttttac ccagtgatat atagtcatat tttaatatat cgttgaatac atcattatct
    49261 atgttgattt tttcattata gaaatccatc aatattttat ataactgatt ctttccttgt
    49321 gccaaatcaa agtagccatt ttcatcaaaa tacttggcaa aaacctcaaa gaatttaaaa
    49381 gggctttttt tataaaatct gcctataata tatctcatag ataacacaaa gtttttagaa
    49441 ttgtagtatc tctccagtat atcttctata tcctttagct tcaaagtttc ttcatagctt
    49501 atataattat tgtataaaaa ctcatatgga gcataatcct tatatttaaa accatgctca
    49561 tctgcaactt ttctcatacc tgtcccttta atcatcttta aaaagcctaa ttgcagatgc
    49621 tctataccta gattaaatac atcattaaaa gaattttcaa agctcttata atcttcataa
    49681 ggcagtcctg caattaagtc taagtgttga tgtatatttc tataagatgc tacagtttgt
    49741 acaacatgag acaacttttt aaaatcatcc cttctaccta ctgcatcaag tgttttttca
    49801 ttcgttgact gaacccctat ttcaaattga aaaagacctt ctttacaatc agccaaaaac
    49861 tcaagcatct tatcatctat caaatacgct gtaacttcaa aatgataagt tgtatatcca
    49921 ttatcatttt tcataagaaa atccatgatt tccatagcca cttttctatt tgcattgaat
    49981 gtcctatcta taaactttat ttgtgatact cttgcatcta ttaaagcttt taaatcactc
    50041 tttatcctgt ctaaactaaa ataccttaga cctggaatag tagatgataa acagtattgg
    50101 cagttaaaag ggcaacctct tgatgattca taatacacta ttctattttc atattctttt
    50161 ggattcaagt tttcataagg actaggaatc tcatccaaat cttgaagtaa ttccataggc
    50221 ttattcacta ttatctcatt gccttgtcta tatacaagtc catttacatc atttatttcc
    50281 atctttcctt gcaagtgaag tactaaatct ctaaaaatta attctccctc tccataaagt
    50341 atataatcca caaaatcata ctttttcatt gcctcataac tatcataact cacttccggt
    50401 ccgcccaaag ctattttagt attaggtcta atttttttta gattatcaca aagtttaact
    50461 atatctccca cattccagat atatgttgag aataatatta tatcatattc atttttatat
    50521 atatccttta aaatatagtc caaatcgttg tttatcgtgt attctttcat attaacttct
    50581 atcaaatctc ttacaaattc ttttagatat cttatagcca aatttgtatg aataaacttg
    50641 gaatttaatg ttgttaataa aattttcaaa tatttctcac ctctttttaa gattatactg
    50701 tctttttgta ccccttatag gttaaaatag tatatgtaaa gtctaaataa tatctatata
    50761 attaatttat taatttcaag taatgtgagg aaaggaagtt ttttatggaa ttaattgaaa
    50821 aagtatctat caactcttta tcaaaaagag atttactact tattataaag tctcttgaat
    50881 acactaatga aaatactcaa attagtgatt tcattgacct taggaataat atagtaaagg
    50941 aattatgttt tttaactgac actaaggaaa aagatttttt agattatcta gaaaaaaata
    51001 gcacactcta ataacctaaa cctttttaaa ctttaatata taaatacctg atttagaatt
    51061 tatttttatg cttaatatat tatcattaaa ggttttcgac ataactgtta tttgattaaa
    51121 tatagggtct actatagtat taccttcttc aagtcttata agacttcttg ggtctatgct
    51181 tttattatat gccttaactc caattttata ttttctcaat ttaacaacat attcggacat
    51241 agaaaaaata aaatctatat tatcttcatt taatttacta tcttcaccca aaatggataa
    51301 tatagatttt tttatgctat taattccttc attttctaca gttctatctt ccatatatac
    51361 atcatataga acatttaaaa catttaataa aatactttct tcatcataat cttcagattt
    51421 caaatcaatg tacttatcaa ttaattgtat tgctttaatc cttgccatct gaaactttac
    51481 aacatctgat ttgtctaatt ctatagaaaa tcctattatc ttgtctttaa tactctgtag
    51541 taattcgaca tactctattt tactattatt tataagattg tgcataatgg aattatacac
    51601 cacagaacct aataaataat caaataaaag ttcttctttt ttaaaataaa taacatactt
    51661 ttttattagt ttaataacct catcttcact tttttgaata ttagtatttg ataaaattag
    51721 tggtaataaa atatttaaaa aattgatatt tcctattttt tctccataaa atctatagta
    51781 aataagttca tctctcagtc tattttcgtc tatatacaca catctttgcg ttttatcatc
    51841 tattaaggat tttgtattta ttatagttaa atctttaaga gccctacttg cactactaaa
    51901 agaagatgtg tagacttttt ttagtaattc gtcattcata aatatatcct ttctgaaaaa
    51961 ttgaatgtaa ataaattgtt attaaaaaaa aagagacagc ccgtttaaga agctgtcata
    52021 tataggggga tagtagtgta caaaaatcat tgtaatctac tatctatata ttattatacg
    52081 atactacttt tttatatgta aagcatcagt tttcgacatc cctatatttt acagttatac
    52141 aaattgcgta ctaagttcaa ttttattctt tttatttcaa cattttatca tgctacattc
    52201 tttcaggagc gtgcattccc aacaatttca gagaattttc taaagtttgt tttgttgcta
    52261 aaactaatgc aagtcttgct tttcttattt ccaaattttc tactaaaatt ggattgtcat
    52321 gataaaattt attaaacgct tgagctaaat ctagcataaa tctagtcact atatgtggct
    52381 catttttctt tagagcaagt aaaatattct tattgaatga ttctataact ctaagaactt
    52441 ctgcactatc ttcatcagct aatagactat aatctatatc agaagtcact tctacttctg
    52501 cttttcttaa aactgcacag catctagcat gtgtatactg aacatatggt ccagtctcac
    52561 catcaaaact aagcgttctt tcccaagaga aggtatagtc tttaattcta ctattagata
    52621 actcttggaa tactactgcc ccaacaccta cttgcttaga tatttcatcc acatttttag
    52681 catttgggtt tttagctagt ataatttcct tagttttatc tatagcttga tttaaagcat
    52741 cttctaaaaa tactactctt ccttttctag tcgacatagt accttcttca agtgcaacca
    52801 taccaaatcc aacatgaatt aaatcttttg cccaatcata tcccataagc tcaattacct
    52861 taaaccattg ttggaaatgt aagttttgtt gagaaccaac tacatatata cacttgtcaa
    52921 aatcataagt ttcttttcta tatattgcag cagctaaatc tcttgtcata tataacgttg
    52981 aaccatcatt cttagtaata agtgctggtg gcattttata ctcttctaaa tcaacaattc
    53041 ttgctccttt agactctttt aaaagatttt tctcttctaa tagttcgata actctgttca
    53101 ttttatcaga ataaaaactc tctcctgcta aagaatcaaa ttctatatct aaaagtttat
    53161 aaactctgtt aaactctttt aaactctcat ttctaaacca ttgccataat tcttgtgctt
    53221 cttcgtcacc attttctaat ttattaaacc atgctcttgc ttcatcttcc atttctgggt
    53281 gttgttcagc ttcatcatgg aatcttacgt ataattttag taattctgga attggattag
    53341 actctactac ttctttttct ccccactttt taaaggccac tattaactta ccaaactgag
    53401 ttccatagtc acctaaatga tttattctta tagtcttgta tccttgcgaa tcatatatct
    53461 tatataaagc attacctata actgtagttc ttatgtgccc tatatggaaa ggttttgcta
    53521 tatttggaga agaatattca actattacag ttttatcttt tccaaaatca ctatgtccgt
    53581 aattttcttt ttcatctaaa acttttttaa ttacagtttc tgctaattga gatttattta
    53641 caaagaaatt tacatatccg cctaattgta ttacttttgt tatctctcca cttggttcta
    53701 tactttccgc caattcactt gctatcatgt ttggagcttt tctaaacact ttggctaatt
    53761 taaagcatgg aaaagcatag tcccccatat ccttatttgg tggaacttct ataagtgcct
    53821 ctatctcttc tttcgataaa tcttctattt tttctttaag gcaatttgat attgccacct
    53881 taaaatcttg cattctgttg ctcctctcta cattgttcct ggtttatatt gtgcttatct
    53941 ctttatgtta aatcaaattt cgtgtttttt caagtatttc attacagtgt aaaacacttt
    54001 agtaattatg ttttctgtat atattaaaaa tactataatt tcagttttta atcacactat
    54061 tattgatttt ttataagact tttttctatt ttattgtttc ttgtactatc cagttgactt
    54121 tatatacttt ttttaatctt ttatctgttt tcatacatca atgtcagctt tatatacatt
    54181 tttttaatct tttatctatt ttcatacatc aatgtcagct ttatatactt tttttacttt
    54241 tttatctatt tttatacatc aatattagct ttatatactt tttttactct tttatctatt
    54301 ttctataaat ctttttcact cttaactttt attccttctt cttctaatag ccttgctgtt
    54361 atacctttcc ctggtatctt tttacctgaa aaactaccat catatattag attacatcca
    54421 caagaggggc taccctcttt tagtattgct tcgctacata catataaatt tgctattttt
    54481 agagtttcat tagcccctct tacaaagttt tctgttacat ccttattatc tttagtaatt
    54541 acttttccat caactatctc agcaggtggt cttggtgtag atagaccacc taactgctct
    54601 gggcatacaa gtgtaaattc tttgcctttt aaatattcct tgactttttc attatcatta
    54661 ttatcgccac tatatttaca atttattcct attaaacatg cagataccaa tatcataaaa
    54721 atgtgctcct atacaaatta tatatattct catacaaatt actaatgtat tcttatacaa
    54781 attattttaa tttaacaatt gttactcctg ctccaccttc tccatattga ccacctcttt
    54841 gtgattttac atgtctattt cttcttaaaa tgtcttgtag ccctgctttt aatactcctg
    54901 tacctattcc atgtattact gttacagact ctaaacctgc cacatatgca tcatcaagat
    54961 acttttctac ttccattata gcttcttcta ggtttaaccc tctaaggtca acttcatttt
    55021 ttacactacc agattttgac cttataatgt ttctcgttga tttagttaca tttgttgaaa
    55081 catctttgcg tgttttttgt aaagatttaa aaggtagagt catcttcatt ataccaattt
    55141 gcactactgc ttcttttctc tttttatcta ctgaaactac actaccattt tgatttaaag
    55201 ttattacctt aacttcttca cctggcttta agtcttttat ctccttattt gatacctttg
    55261 gaactatcat actttttact gttggctgaa gacttcccat agagctagtc aattcttttc
    55321 taagttcttc tatcttacga ttcttttctt ttgaagctct ttcttgttca agacttctaa
    55381 gttcttttat tataatatct acttcttctt tagcttgtct tataattgaa aatgcttctg
    55441 atttagcttt ttcaatcatc ttatctcttt gactgaccaa tttttcaagt ttttcgtcat
    55501 actctacttt cagtttttca atctcttctt ttaatctttc agcttcttct ctatcttcta
    55561 ctgctttgat tctattcttt tcaacattct gtaacacatc ttctaaagct atgttttctg
    55621 tatttatata ttctttagca cgtgaaatca cataatcact tagcccaagt tttctagaaa
    55681 tctcaaacgc atttgatttt ccgggaacac ctataagtaa cttatatgtt gggcttaaag
    55741 tttctatatc aaattcaact gctgcgtttt ctacacctgg tttagtaaga gcataatttt
    55801 taagctcact atagtgagtg gtagctatac atttagctcc tacagaattt atatcttcta
    55861 atactgctat tgcaagggca gctccttcaa cagggtctgt tcctgctcct aactcgtcaa
    55921 atataactaa agagtctgct gttacgtttt gtaatataga tactatattc gtcatatgag
    55981 atgaaaatgt tgatagactc tgttcaatac tctgctcatc tcctatatct gcaaatacat
    56041 tatcatatac acacatacta cttccataat cagcaggtat atgaagtcca ctttgtgtca
    56101 taagtgcaaa tagacctaca gtttttattg taacagtctt accacctgtg tttggacctg
    56161 ttatcaccaa tgtatggaag tctcttccta aataaatggt atttgctact accttctttt
    56221 tatccagcag tggatgtctt ccatttttta tgtttaaata tttatcctca tttaaagttg
    56281 gttctattcc tctcatctgt atagaaagtt ttccctttga aaatgcaaag tccaatcttc
    56341 caagtatctc ttgattagag attaaatctt cgctcacttc tccaaccata gcagaaagct
    56401 ctgataaaat tctttctatt tcttcttgct cacctagttt taactgtctc aattcattgt
    56461 tcatctctac tatagtcatt ggctctataa aaagtgtcgc accagaagat gactggtcat
    56521 gcactatacc agctacttgc gacctatatt cagattttac aggtactaca aatctatcac
    56581 ctcttagaga aattattgca tcttgtaagt acttttgata agtagttgaa cttattattg
    56641 aatttaattt tgacctaatg gattgatttt tttgggctat tcttcttctg atatctctaa
    56701 gtgtacttga agcattgtca gatatttcta cttcactaat tatagcattg tatatctggt
    56761 cttctatgtc cttgtataca tatagagaat ttgatagaga ttgtattatt ggataattaa
    56821 aatcttcttc atcactacta gataagctat ttcttaatct tctagccact cttaaggtat
    56881 ctgctatcat aatcagactt cctgggtcaa gagaagcacc tatataagct ctctttacct
    56941 tatcttctat atcatgaata ccttccaacc ctactgaacc tctctttata ataattgatt
    57001 gagcttcact agtttcttct agcatagatt tcacttctac aaaatcagta tttggtacta
    57061 aattttctat atattttaga cctaaagaag atgaggcttt cttctttaac aaatctatta
    57121 ttttattgta ttctagaact cttaacgatt tctcattcat tttctatatt caccatcctc
    57181 gatattttaa cttttttatt taattttaaa aatgtatgtt ttcaagcatt tccaataaaa
    57241 actgcaagtt ttatttcttt ttcatttcta aaattattat attttttctc ttttagatat
    57301 attttacaca tattttgtat tatactatgt atgtagctat ctatatatta tacataaaca
    57361 tgctttattt gtaatataat ctgttaaaaa atcttcttta atatttttaa aggagtttat
    57421 tttagcttat aaaaaaaaat agctacttat atagataaat taaggaggaa catgagtcat
    57481 gggaaacgta ttagaaggat taaaaccaga aagtgttttt aaaaactttg agaaaatatc
    57541 tcaaattcct agaggttctg gaaatgaaaa aggcataagt gattttttac ttagctttgg
    57601 taaaaattta ggtcttgaaa ctattcagga tgaaagctta aacataataa taagaaagcc
    57661 tgctacaaaa ggatatgaaa attgtccagg tgtagttctt caaggacata tggatatggt
    57721 ttgcgaaaaa gaaaagaatg ttgaacatga ttttcttaaa gaccctataa aattaagaat
    57781 tgatggtgac atgatatatg ctactggaac tactctagga gctgacaatg gtatagctgt
    57841 tgctatgggt atggctatat tagaagataa tactttagag catcctgcac tagaagtttt
    57901 agttactgtt aatgaagaag atggtatgaa tggtgcagat gcactagacc ctagcttaat
    57961 taaaggtcaa tatatcctaa atatggactc cgaggaggaa ggatacttat tagttagctg
    58021 tgctggtggt aaaacttgtg ttgttagttt acctgttgaa tacaaggaag ttaaaggtga
    58081 caaacaagga ttattagttg aggttactgg actattaggt ggacactctg gtatggaaat
    58141 tgttttacaa agagcaaatg ctaacaaagc aatagctaga gtattaagtg tattgaatgt
    58201 tgattatgaa ttagcttctg ttgatggtgg tactaagcac aatgctatac ctcgtgaagc
    58261 taaatgtgta atagctgtta ataaagctga tgttgaatct gctaaaaaac aaataaatga
    58321 cattttaact gcatttaaac atgagtttac tacttctgac cctggcatga cttactcagt
    58381 agctgaaact tctgtagaca aagttcttac taaagattgt aaagaaaaag ttgttcaaat
    58441 gtcttgctta actcctcatg gagttcaatc agtaagtctt gatatagaag gattagttga
    58501 aagttctact aacttcgcta taatcgaaac taaagaatct actattgaat ttttaacttc
    58561 tgttagaagt tctgttatgt ctattagaga tgaaatcgct gatagaataa gacttttggc
    58621 tcaagcttta ggtgctaatt acgaccttat agctcaatac ccagcttggg aatttaaaaa
    58681 aggctcaaaa ttagaaaaaa tatgcagtga aacttatgag aaattaactg gtaaagttcc
    58741 tactgttatg gctctacatg ctggtcttga gtgtggatta ttacttgata aacttcctca
    58801 tgctgaagct atctctatag gaccagatat gtttgatgtt catactccaa atgaacatgt
    58861 tagtatccct tctgtagcta atgtttggga ttatgttatt gaaatattaa aatctatgaa
    58921 ccaatattaa atttaagttt tgattctaag tagattttaa ttttaagtaa tctatataaa
    58981 attaactctg taaattaata ctacggttag tataatattt actttcaata ttaacttata
    59041 gaaataaagc ctaatgatag ataataacat gtatcattag gctttattgt aatttaaata
    59101 ttattaagct ttattctgtt ttaaaattaa ttcttatact attttaaatc tatttcttcc
    59161 ttgtctctta gaatcataaa gagcattatc tgctatttga tacagttcat catactcttg
    59221 accaggcttt gctataacta cccccatact aatagataaa ctttctccat ttggcaactt
    59281 aatgagatga atatgattgt atacatcatt acatttatta ataagttctt ccttatttat
    59341 tatatcattg atataaacac taaattcatc tccacccaaa cgacctacta tatcattttt
    59401 tgcaaaactt tcatcaagtg cctttgcaac atcaataagt actttatctc ctaacaagtg
    59461 accataagta tcattaatcg atttaaaata atcaatatct ataatcaata aagcaccatt
    59521 tttttcagtt gataaattca acaaattttg tgaagttaat tttctaatat atgctgcatt
    59581 ataaatattg gttaaactat ccctttgagc cttatctaac aatctattct tttcctcttt
    59641 ttcagaatca atattacata ttttcccgat tacatacatt ggtatttttc tatcattata
    59701 aattattttt gaagtaactc taatccaatg aaaagtatca tcaagcataa tagaatacat
    59761 ttcagttgtt ccattttctt gtgattttat tacatcaaaa aactttttat tatattcttt
    59821 ccttatttta tctctttcat tttctatatt gccattttca ctttccttat catttttatc
    59881 caaaccataa taagtctcaa tacccttttc tggcatagaa attatcaatt tatcttgctt
    59941 ataatcatac tcaaaaaagt attcacttgt catttcatac aactgagtat gttttttgag
    60001 ttctaaagat atcttctcgt tcatacttgt acgagtatgt aaaccaaatg ccataaatcc
    60061 aataatacaa attaataaac tggctacaat aaaaatagtc tcaattggat tctgttgcat
    60121 aatataaagc aacgagaaat cttgtctata tgtagtattt tggtagacaa ttgattgcat
    60181 tttttctgat gaaatactta taatagcttt attaaatata ttcaataatt cttgtttatc
    60241 ttttttaatt ataccaaaac aaatactctt tgctttatag gtttgaggaa ctagacgcac
    60301 atttttatac ttaggttgat tcatatagta ctgcatagaa taaccttcgc cataagtgta
    60361 atctgcatag cctttattaa cagcttctat acactcttct aaattatcat accaaactat
    60421 attctcttca aactgaccat aataattaat acctttggct agagctacac gttttccatc
    60481 taaactattc tcatttaatt tattattaat catcatagta tattgagtag aaagaaaagg
    60541 ccgagacata gctatattat gttcacgaga gtattcataa tcataaatca ttcctgctac
    60601 aatatcaatt tcacgctttt tagtcatgtt ttctaaatct tcttgagatt ttacaggcac
    60661 aagttcaaat tctaagcctg ttttatcaca tatatgattt aacaaatcaa ttgatatacc
    60721 tcttaattca cctgtatctt tatctatatt ttgaaatggt ggtttgttta aaagtactcc
    60781 tacttttaat ttttctgtct ctttaatgta attagtttct tcatcagata aaaacatttg
    60841 attattctct ggtgtaaagt atttttcata taaagtagtt gaaaaatatg ggtctttctg
    60901 ttcaatatta ttaattgctg agttaagctt ttgagttaaa tttgtactac ctttaggagt
    60961 aatgaaataa aatggttttg atgaaaattt tgctacagta cgtacacctt ctattgggtt
    61021 catactcaca ttaagaagta catctgcttt accactcttt agtgcttcta gttgctcatc
    61081 atttgtttta caatatacca attcaggtga cataagattc attttacaat actcatctaa
    61141 ttctttaagt ctagtatcag aattttctat tacagcaata cggatttttt tcacatcttg
    61201 tgaactcatt atgttatcaa tatctgtatt ttcatataat gcttgtagta ctgtatttgc
    61261 tactccataa ttattccctg cataattata tattttttgc attggttcat tgtataacat
    61321 acctcccatt atatcaattt caccattttc taacatcttc attaattttg taatagattc
    61381 atttatctct ccaggaactt gaacaaattc ataattccat ccagtatact gtgcaatttc
    61441 tagtaaatat tcatatgtat acccaaaata gttaccttgc tcatctattt cagttaaacc
    61501 tttttgaata ggataagcaa cacgaatggt acgattatca ttacctatag cgttgatacc
    61561 aaaagaaaat atgcaagaag tgaatataga tgtaattaca ataattaata aaagtaattt
    61621 ttttaaattc actacacaat cctccatata gttaagattt acagtataaa ttactgcata
    61681 aacttgtaga catgtaaaaa tagattagta tataaaagga tatataaatc tacttagaaa
    61741 tttaaatatc cttaatatat acaatatatt taaattataa ctcatttata tgtctattac
    61801 aacaaattta ccctgctaaa tacctactct atatcccata attttacaag ttgtagtgaa
    61861 gtttaagttc tatctcacct caaatttagc agtgtttcta tttacagttc ttttatattt
    61921 cacatctgga taacccaata taaatgaagt gataactgct tcgttctcgc tcattcctaa
    61981 cagttctttt atctttggct ctacatttga tgccataaca aaaaatccat tataacaaat
    62041 tcctaatcct tgagaatagg ccataagttc catattagat gcagccaatc ctccatctac
    62101 ataagcactt ccacctagag acatatcacc tacaactacc ataagtagtg gggcatcata
    62161 aaataaagaa tcatttccgt tctctttgta tcttttgtac attttcttaa atgtattttt
    62221 atagatagaa cgtacagggt cattgtcatc agtatctaat gccaaattat aaagaccttg
    62281 tattgccagt tctttcactt cttttagctt ttcttttact aatatgtatc ttataggctg
    62341 tctattacca cctgttggtg tgtatctacc agcttccact atgtttttta ttttttcttc
    62401 ttctacctct atatctttat attgtcttat acttcttcta aatttaataa aattcaataa
    62461 cttttcgggt tctaattcaa aactttcacg attgtattct attacttcat ttttatcaaa
    62521 tccttgcatt tctattgcat ctgttgggca aattgccata cagtgaccac agttaataca
    62581 gagaatattt ttaacctttg ctttatcatc cttaactttt attgcattta caagacagtc
    62641 tgattcacac atcccacagc ctacacattt ttctaaattt atgtctatca ttccaaatta
    62701 ttcctccttt taaaaaattt actttagtat aaaacaaata gttgtctaag tcaattttta
    62761 tttttttaac aatatactag ccttaactac actttaatgg agaaaaatat acataaattg
    62821 gaacttaaat tagaacttat tcaaagtaaa agataaaaga agtagatgtc taaaagtgaa
    62881 ctttttagtt aattagatat tctttattgt atgaaatcaa gtggatattt atcatttcaa
    62941 caataaaaaa ggcttacctc tattttggat atgtccctgt caagtagaca gactaaaaaa
    63001 aggctatctt tatgcactga gatagcccct tttcccaact actattgttt atcttaatat
    63061 tagtcaataa atttaatttg ctataacctg taagttacat tttaatttga tgtaacttta
    63121 aattacattt ttaagtaata tctttaaatt atattcttaa aatatatcat taaactacat
    63181 tttaattaat ctaaaaacta tatcttaatt aatctaagaa acttacatta taatctttta
    63241 gtttcccatt atcatataca atctttgcac taaagatatt tccagtctta gcccaattta
    63301 agaacagcct gtcaccttcc cacatattta aatcgaatat tttatcatta tcaacccaaa
    63361 ttaaatttcc ttctttacaa tcatttaaat ctttttcaga taattcacct tcaaactcat
    63421 ctgagaaata caaaaacata taccaatctt cttcatcttt aaattttgga aaagttatca
    63481 accctttata tgacaatgat ttaagagtaa gaccagtctc ttcttttact tctctaatta
    63541 cacattcttc tggagtctct ccttgctcaa actttcctcc aactcctaca tacttacctt
    63601 catgtatatc atctttcttt ttatttctgt acaacatcaa agtcttgtca tctttttcta
    63661 tataacaaat agttgtaagt ttcatgtatc tcgccttgct ttctctttta tattctcaac
    63721 taattatttt cagcactttc aagcacatca cttatattct tcattgcatt aagagaaagc
    63781 tctgcaaatt catcaaatgg aatccctata tcttctatac ttctcattgc atctctattt
    63841 acatttgaag caaatctcgt ttctttcatt cttttaataa cagacttagt ctttacactg
    63901 gtaagttttt tatctggata aactaaagct atagcagtta taaacccagt tatagggtct
    63961 gctgcatata ttgctttttc cattaggcta gttcttgtaa ctcccattcc atcatgatga
    64021 ccaagtattg ctctatacat ttcttcgtta ccgaagtttt cttttttaag cagttctact
    64081 gttgttgtag catgtccttc acatatttct cctggctttc ttccaactat atctgaatcc
    64141 aaatcatgta aaagacctgc cactgcccac tcttcttctt tttctggctc tagtcttttg
    64201 gctaattctc tcataacagc ttcaacagca taacaatgtt ttctcattct atcagtttct
    64261 aaatacttaa ataaaatttc taaagcttct tctctcattt tcaccaatcc ccctgttttt
    64321 tcacatatat gatattttta tatattaata cacataaatt caagctttta tatatattca
    64381 tgtatatttt tcatgttata tctattctac acctttatag tagtgtcctg caactgttcc
    64441 aatttcatct aatttcttat acaagtctgt agcctttttt ccaagtttat aattattact
    64501 cagtacctca atgtgagctt caataacctt tttaaccatc tcactatctt caactgtaaa
    64561 atctatccta aatacatcta ttccaatttc ctctaactca tatagattgt ccaacataca
    64621 agtaaccttc gaattgtaaa ttgtacttct acagaatata tcttgtgtta tcctataact
    64681 tgcatccgta aagtctctta aagcataact actttcctta catttagcaa ctctcttgtc
    64741 ctttttacaa tctctaacaa ctactcccat aggacagtat tctgtaacca tcataggagc
    64801 atatccatat attactgctt caatttctac atctgtatat tttaaagttt gtttaatttc
    64861 atttaagttt agttcttgtg acaaacaaac tgtacttgct ccctcttctt tgaagtgatt
    64921 tatactttca ctattgaata catttaagta gcaatctata tttaagtctt ttttcttaaa
    64981 gtattccata gagccccagt taccaatttg aaccttatta attcctttta tattatccaa
    65041 tatctcaaat tgcttgtact ctttatttct aacaatcctt ggtgctgaat agattaattt
    65101 tttgtcagtg actgctgtat tagagtggtt cttattcaaa gtatctattt tacttgtatt
    65161 tgacaaagta tctatgttat ttgtatttga acccattagt tctttaggag tacctaaaga
    65221 cgaagaagta tctaaatcca tagaaatact tgaatctata gaaatatttg aatctataga
    65281 gatacttgag accatttgta aagcttttga taatgtattt atatcttcat aatagattaa
    65341 atctacatta taatttaata ctgcttctaa ttgctccaag ttcttaactt taactctaat
    65401 cttaggagct gtatcaagtt ttttctgttt atttactcta tttggctggt attttaatct
    65461 attcttctta aattttctat cttttatctt tattctttct tcatctaaaa gctcaataca
    65521 atcccttctc atttgattta acacacttat aggcatactt acaccttcat ccaggtcaat
    65581 ttctatctct tttaacttat atggtgtatt tccaagcttt tgaatttgtg tttctatttt
    65641 ttctttactt aaagccacct ttatagcttt ttcaactatt ttgtctcctt ctaccaccga
    65701 tttatttcca caaaaatcac ttataaaaag ctctggcttt ttacctagtt taatactcac
    65761 ttttgcacgt atattagttt ttatgaactc tttatcttgc tgaaatgtag cttgtactct
    65821 atccattaat tcgctgtcag aagtcttata tataagctga tttctctttg cttctccaac
    65881 aaaatctaat tctatcaaat cccctttatg tgctattgta gttatctctc catcctttat
    65941 tattcttcca atagtacctc cacctaagtt aattccatca cctttcttaa gcgtattttc
    66001 tagcattact ctaagtcttt tgttctttct attataatct aaaacttttc caatatatag
    66061 cccttgatta ttaggtctat tagagttcat tatatcctta ccaacttcac ctaatatata
    66121 tccttttgta aactttctat taaatatggt atacaaatta tttattattt cctcagaaat
    66181 acttattttt ttcttatcta caaattcatt aatcgcttct ctatatcctg aaaccacagt
    66241 agctacatac tctggctttt tcattctacc ttcaattttt aaagataata cacctgcttc
    66301 aattatttta tccaaatctt ctatagtact taaatctcta ggactcaaca aataatctcc
    66361 attacttttt actacttctc ctgtatttat atctatcaac tcatactttt gtctacatgg
    66421 ctgtgcacac ctacctctat ttccagacct attgcctatc atactactca taaggcattg
    66481 acccgagtaa catacacata aagctccatg tacaaaaact tctatatcta cagttgtatt
    66541 ttcacaaatc cattttatct catcaacatt taattctctt gctaaaacaa ctctatcaaa
    66601 ccctacactc tgaaggtatt ttacatcttc taaggaatgt gctaccattt gagtgcttgc
    66661 atgtagttca aagtcaggta atagtttttt tataagtctt gccattccta tatcctgtac
    66721 tattaaagca tcaatatcta tatcgtataa aaattttaca tactctacaa aatcttctat
    66781 ttcatactgt tttatcaatg tatttacagt aacaaagact tgcactcccc ttatatgaga
    66841 gtatttaaca gcctcaatca gctcatttct atcaaagtta tttgcagatg ctctagcact
    66901 aaagtcttta ccacctaaat acacagcatt tgctccgttt tgaactgctg cttttaatga
    66961 atcaaaagag cctactggtg caagtaattc tatttttttc atatttttct cctctattta
    67021 tacttgttag gttaatataa ttatatctat aacttaaata tttgtattaa atttatatat
    67081 ttcttactat attttaaatt cttcaatcaa tttatatatt agttatttat atttggttca
    67141 tttgagttaa catatacatt atttactata tttagtatat ttctattaac ttatatattg
    67201 attactgtat tttaaattct tcaattaatt tatatactgc ttactaaatt taaaacattt
    67261 taattaattt gtacactgtt tactatactt gaaatacttc tattaatcta tatattgcct
    67321 actatactta agacatt
//
LOCUS       DAEMGG010000006        64956 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEMGG010000006 DAEMGG010000000
VERSION     DAEMGG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64956
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.006"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(51..209)
                     /locus_tag="KPK24_000358"
     CDS             complement(51..209)
                     /locus_tag="KPK24_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298073.1"
                     /translation="MDNFLQNVLERLSVSLIVCLVSNLLKKRKKPLKVAAKSGWEFDF
                     KIKFRRFK"
     gene            complement(385..588)
                     /locus_tag="KPK24_000359"
     CDS             complement(385..588)
                     /locus_tag="KPK24_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298074.1"
                     /translation="MRESLLNKYNLKTDEDVEYFVKFANALYELKQNSEEKFQEYVKI
                     LREILIEQQENENILDNILKTLT"
     gene            complement(651..1472)
                     /locus_tag="KPK24_000360"
     CDS             complement(651..1472)
                     /locus_tag="KPK24_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HBG2298075.1"
                     /translation="MKNELMMFEEKKVEVLEYNGQVLFNPYDCGRCLELSDSAIRNHL
                     SKMNDTQAILLKNSAVLDKDIRKLNNAGEKFLTESGVYKLIFKSQKEEAEKFQDWISD
                     EVLPAIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLKELLDDAGFDNKSKLL
                     TAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPAQMAVCEIIKKIDLEE
                     NEVKGVWETNGSWTGTVNKYTKSVIDKVRTWIEENNRPVKIAGEKKNYHVVYKIE"
     gene            complement(1592..1780)
                     /locus_tag="KPK24_000361"
     CDS             complement(1592..1780)
                     /locus_tag="KPK24_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298076.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     repeat_region   1931..2482
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2061..2089
                     /rpt_unit_seq="atttacataccacatagttaatataaaat"
     gene            complement(2740..3660)
                     /locus_tag="KPK24_000362"
     CDS             complement(2740..3660)
                     /locus_tag="KPK24_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG2298077.1"
                     /translation="MNFFKRKDDIKNFKLMYIKGHPHFNNELTCTMYIDKKLVSVYTL
                     QQVPISEIKLLDIEKVFIDNDMLIIESKKSVLYFKSNSKDKICNIINQNIADMQDKKT
                     DTILRNKNKIVKQGVMKMDYYNSVGVPNCRGEAISIIIDNHNACLVIESKLLKEEKMR
                     IKFSDLYSVNKYSEKEIAYNNKSVVGRAAIGTLFGPMGTIVGGLSGTGNKKSKTEKLF
                     ISIEYFSHGELREIIAEDSILNFHSQEFIDNIRRKINISKNNLENNKEKQIKSNDDAI
                     QQVKGLKELLDMGAITEEEFNKKKKELLNL"
     gene            complement(3740..6925)
                     /locus_tag="KPK24_000363"
                     /pseudo
     CDS             complement(3740..6925)
                     /locus_tag="KPK24_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            complement(7082..8077)
                     /locus_tag="KPK24_000364"
     CDS             complement(7082..8077)
                     /locus_tag="KPK24_000364"
                     /inference="COORDINATES: protein motif:HMM:NF022337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBG2298078.1"
                     /translation="MAKKLTNEEFIERIRKIHKTEIKVLGIYKNKRTKVLVKHKCGYE
                     WEANPESLWSGSCCPLCSNNLRKTTDKFKSEISSLSGTEYEIIGEYINSSTPILFKHN
                     LCGKTFKMRPTDFLKGQRCPYERYKKSANSNTINFNIIKENVEQLGKGDYKIIGEYRG
                     ASKKAKFFHVSCGNYFYMEPTRFINGGIRCPHCYRSKGEEVIREYLNENKFDFKEQYK
                     IKECKNKRPLPFDFAIFKKSEIKCLIEYDGSQHFMPKFNCSKEDFQKIKYNDEIKNEF
                     CKKNNIPLIRIKYVRSENLNIIKNKIIKKLEDGFEKINMGIPSEASLETTGTCND"
     gene            complement(8180..9292)
                     /locus_tag="KPK24_000365"
                     /pseudo
     CDS             complement(8180..9292)
                     /locus_tag="KPK24_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            complement(9473..9946)
                     /locus_tag="KPK24_000366"
     CDS             complement(9473..9946)
                     /locus_tag="KPK24_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298079.1"
                     /translation="MGNLDKEFLNEGIEEERELTKDEIAKQQEDNIIMKLTEDAILPE
                     KTIFVKRLDIPLTLRALTEKEISALQKKYTKVTKVRGRRESKLMEDEFNIALIEKATI
                     VPNFGDTRLLNSMKVSNGVEFIRRKFLAGEIALISDEVLELSGFYEELSDGDIKN"
     gene            complement(10074..10388)
                     /locus_tag="KPK24_000367"
     CDS             complement(10074..10388)
                     /locus_tag="KPK24_000367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298080.1"
                     /translation="MKVILSQKMSMHNMFGKREELIITKQVDTDIIPRIGEYVRDEIN
                     ISNHDTFKDSLKVGEVIYNFKDNECYVNFEKRSINFSNDEDVSKKIEQLKEMATKHGW
                     KY"
     gene            complement(10501..10923)
                     /locus_tag="KPK24_000368"
     CDS             complement(10501..10923)
                     /locus_tag="KPK24_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298081.1"
                     /translation="MGKYDENIIDAANVVDGSNARIIIDGEEEGYGTEFTAEVENDKK
                     TFRVIGCKWELNKASTQKGTFSLTVLKTTSKWIKKGFNKFEIITEIENPGLVGYERIR
                     YKNCMVDKIQLASIKSDENIEIQIDGTFEGFELLDFID"
     gene            complement(10939..12360)
                     /locus_tag="KPK24_000369"
     CDS             complement(10939..12360)
                     /locus_tag="KPK24_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG2298082.1"
                     /translation="MATGTWNEKEKKEIPGFYNRFKTQAEKSTNKGLKGRLAIPVKAN
                     WGEVGKVVTIKNDLRQLKTLFGDDMNYSAYKLGKLALLGNVKELLLYRLVDGNQKKGT
                     LTLKDTTENTAKDVIKLETKYPTSRNFNVTIKSNLVDADKKDFIFFEGTKQLFSSSVK
                     GTIDEIVLEINSNLDNEYVIATKIADSDTTLANLVNVALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVADEALQETTKAWVTKNKELGKDILLFLGGKKEDNIKQINDKS
                     KGFNDENIVNVGSSAYYENVKYTPSEVAVYIAALSVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLVLDFDDGDVIIVDDVNTFKKYVDDKNEAMGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDSTGQTTVICALKKYFEELMSQGIISEFNVDIDTELQATAKADE
                     FYWKWDAIKVDVMKKIYGTGYLG"
     gene            complement(12362..12565)
                     /locus_tag="KPK24_000370"
     CDS             complement(12362..12565)
                     /locus_tag="KPK24_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298083.1"
                     /translation="MAETINKKTNVSKQEEKYLKSDFIKNSEALGYKKEVVAGALFNC
                     KKDELTKSEFEKLIKDFLEREVK"
     gene            complement(12578..13402)
                     /locus_tag="KPK24_000371"
     CDS             complement(12578..13402)
                     /locus_tag="KPK24_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298084.1"
                     /translation="MRAGIRKALIENIPKLKDCYEPTVPNKKTIKPYAVIVQGEDADN
                     EGDVIGFRRTINIWLYEKRTTFNKLDELTKEVIETLDFKTITDNTSNEVFTCIYEGAV
                     GQDVIDEEWEAIIRCLRFSVIALDDKEDITNDRWVEALSKYTKDLLEIESYKDNWKKN
                     FIAPCCLWRTTNIENKRINYHLIEITKTMKCHVVSKNKDEIVKLLEELETKLIIDKRV
                     RLREDKNMYLTLVSVVEDRESDMFSNGQLTVVFKMIGKIKREGPIMNKIYSNGNLR"
     gene            complement(13404..13814)
                     /locus_tag="KPK24_000372"
     CDS             complement(13404..13814)
                     /locus_tag="KPK24_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298085.1"
                     /translation="MSVFTKAINEIDRKKATMPLLCMNIAFMLEGEAKNSAKWTDRTG
                     NARQGITGTSLGGGNQYIVRLGHGVDYGTVLEEGSAPHIIKPRNAKALFWNGASHPVM
                     KVQHPGTKGTHSLESTISRNMPKIGKLIEGHWSK"
     gene            complement(13827..14210)
                     /locus_tag="KPK24_000373"
     CDS             complement(13827..14210)
                     /locus_tag="KPK24_000373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298086.1"
                     /translation="MRKLRADKIIKTINRGIALNPQTITIEQEVKNIVDGAIEVTNEV
                     KELTVVIYPEKTNDTVINSETIGTAYKNKNFGMVTDKEADLRLNTESNITFKCIEGTM
                     KLNYVNPVVVEGKICGYICGLEKLD"
     gene            complement(14207..14524)
                     /locus_tag="KPK24_000374"
     CDS             complement(14207..14524)
                     /locus_tag="KPK24_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298087.1"
                     /translation="MSITNLDKLKLNLQEEEYPYFTDEQLVMLLESNENNVLKASWRG
                     CLLKGATDDCIKIGPIETKSSNSSYWLSLADIYKTDYLEEKSKNETTNTGYKTSMIRV
                     DGQ"
     gene            complement(14527..14733)
                     /locus_tag="KPK24_000375"
     CDS             complement(14527..14733)
                     /locus_tag="KPK24_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298088.1"
                     /translation="MAKKKDNLMQVKALVYLKYDNGCYKIGDVFEIRKADQESMEEKG
                     YIEVIGEIEEKNNSDNELLEKDGE"
     gene            complement(14751..15779)
                     /locus_tag="KPK24_000376"
     CDS             complement(14751..15779)
                     /locus_tag="KPK24_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBG2298089.1"
                     /translation="MDWKDFIDSKEIAKYIKKLPLEMLIGEALFPRKKQIGMDLKYIK
                     GAKKKPVVLKQSTFDVAVKIRALKAQIEVKSKRMPFFKESVLVNEEDRQQLLLASQAQ
                     NKELLLMIISQIYDNYLALVDGGDMQMERMRMQALADGVINIVSEDGDLVFDFEVPSN
                     HKEVLTGSATWDNPDADIIGDIQRWMRIMRDEGNPLPKRMVMTSKTFGYFAKNKAIKL
                     DIDKDGRVILTDEMIKNYLKNKVGLSVAIVSGTYKLEDESEESYFPDNKITFIPDGDL
                     GKTYYGTTPEEADKVYGSKLDCSVVRTGIAITTMRLIDPVTVQTKVSQLGMPSFERAD
                     ECFFATVA"
     gene            complement(15803..16159)
                     /locus_tag="KPK24_000377"
     CDS             complement(15803..16159)
                     /locus_tag="KPK24_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298090.1"
                     /translation="MSIEKSEIYMGENKTILKFAGNLFQNVNIKVKKTDVAIVDEKRI
                     LKAGTLISKDGKLVDGTTVTNDKAFGLVYRDIDFTYSNGNESIPVFIFGFIDEKTLPT
                     AIPEEAKQAMKMIMFL"
     gene            complement(16171..16824)
                     /locus_tag="KPK24_000378"
     CDS             complement(16171..16824)
                     /locus_tag="KPK24_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG2298091.1"
                     /translation="MLEYFKKLLGDEEGQKVYEKLSKDKENKLLLDNIKSPRYVEKTE
                     LENANKEIKEYKKQIGDRDKQLNDLQGKVKDNKELSDEIESLKNANKEIRENAEKEIE
                     VLKFNTAFDRAIESYNPRNPKALAALINKENINFVDGKIIGLDEQIKAYQQSDSYLFN
                     NEKNKGGDEIGGTGGLEGGTTSLLDNDSEISSIGELLANSKVKSENIEAQKKFFGEE"
     gene            complement(16892..17044)
                     /locus_tag="KPK24_000379"
     CDS             complement(16892..17044)
                     /locus_tag="KPK24_000379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298092.1"
                     /translation="MLEEMRKDKKLLELRRLYKEKYGKNAPGFNYDEYNSYAEYKEKL
                     KELIQK"
     gene            complement(17046..18665)
                     /locus_tag="KPK24_000380"
     CDS             complement(17046..18665)
                     /locus_tag="KPK24_000380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298093.1"
                     /translation="MKDNAYTKKVLEARKKLLLLDKKVQLDILNVYKDASKTILSDIA
                     KNKELNLSTKYLKKLNKSIEKYINELNQRLVPITEKSVIEASNISKELQMYYYQSIVP
                     NKSINLACDAMCIKTTTSVVEKLVTGNFYKDKRSLDSKIWGYSNKNRKDIDRLIKANV
                     ARGANAKTLAKNLDNYINPVRKTEAKTLEVGMNKSISYQAQRLARTSITHAFVETSVQ
                     NAINNPFCVGLQWNLSSQHYIRQVKWRGEDECDEYAEQNRFGLGEGVFPPEKYPIPHP
                     NCLCYPVQVIITINEASKMMNDWVTGGDNDILDSWFDGVIYQNNNVPVIKKQRNKKNK
                     ITANSKDDRIKKNITNSIKNKSKKAPKQVQKYINKYVNPNRIIIDNSQKIPFVYYTKV
                     DLVGINPNIREFKSYNKEAALLHEFAHRIDIKEIKSYNNIKFQKAIESSSIYVMENIE
                     NLQSIYTNSNELYNNEFISDILGALSNNEFEDLLATHSEKYWSKNRNKEKEIFANLFT
                     LKYQNNKEINGFIKEHLNSLDKIFNELLGGI"
     gene            complement(18678..20201)
                     /locus_tag="KPK24_000381"
     CDS             complement(18678..20201)
                     /locus_tag="KPK24_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2298094.1"
                     /translation="MNIKNILLNLDEKELRDRKHAERDFLFYLGECRNKTMGLLDDDF
                     LGQSWITFDNLDYTPSQIVDNKVKPLINKQARFMFGKEPTIILKAYEKEHKEACEELR
                     QYIDSILNASKFWSNTLKAFKIATITKRVLLRLEAKPNQPIRLFYHSINDFKYQVDSN
                     DITKLKSVVFVRFDSSTIKEVTAKQIWYRYTYYMKKSNVSNQESCFIKIEKFKGDNLS
                     KPIEVKESDTKLSKIPCWVIVNEQSITNITGISDIEDLKPLQDTYNKRLSDFNDSLKF
                     LMFGQTVVVDATEETVNACKIAPNALMALKTLEEGSEKGKQAQAYRVESSFSNADPVN
                     SFFKRLEDSMYEKLAIPRPEQLQNIPSAKALKYLYTELIARCSEKWNDWEPAIRSMLR
                     LIVEACSKFNCYDDWNHDWDDLMFSIVLNKNYPIPEDEEDSKRLALEEVNNNVRSHRN
                     YIKEFGDDEDYEEAFNEVLEDNEKIQSVEQDQFRKDADIEVDDIDEELNSKSNNSDE"
     gene            complement(20201..21562)
                     /locus_tag="KPK24_000382"
     CDS             complement(20201..21562)
                     /locus_tag="KPK24_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG2298095.1"
                     /translation="MKLALLRRKQKRQEKRFEFKPFSKKQLKLLNWWREGSKYKDYDI
                     IIADGAIRSGKTIAMICSFLMFTQTNFEVENFIIAGKTIGSLKKNVIEPMKQILNAWG
                     WRFEYNRSENFLVIGSNTYYMYDANNEASQDKLQGLTAAGALADEVALFPKNFVDQMI
                     GRCSVDGSKIFMNCNPAGPYHFIKTEFIDKVKEKLICYLHFTMDDNLSLSEKVKNKFK
                     RMFTGVFYKRYILGLWCQAEGVIYDMFNEKIHKVLTKLREYTEHYVSCDYGTQNATVF
                     ILWGKCKDVWYAVKEYYYDGRKEGKQNSDNKYYTELVNFLGNIHPKAIIIDPSAASFI
                     TLIRDKGKYRVKKGNNDVLNGIRNVGTALNREMIKFNDCCSNIFKEFFSYVWDEKALE
                     YGEDKPVKVMDHAMDAIRYFVHTILFGGKEPNYDDEIYNKGLGLKKNNIQNQYNKKGG
                     TVF"
     gene            complement(21543..22304)
                     /locus_tag="KPK24_000383"
     CDS             complement(21543..22304)
                     /locus_tag="KPK24_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2298096.1"
                     /translation="MNEKADLAHEDYLKGLKYKEIAEKHNVSLSTVKSWATRYWKQKG
                     CNQPKKVATKKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELIQEISIKDKFD
                     ILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASF
                     LNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRIDTMRTKVELEKIKLFGDDKYD
                     NSEAIQSFLEATTMSEEDIKVLFNEEEGENEIGST"
     gene            complement(22395..23552)
                     /locus_tag="KPK24_000384"
     CDS             complement(22395..23552)
                     /locus_tag="KPK24_000384"
                     /inference="COORDINATES: protein motif:HMM:NF037255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298097.1"
                     /translation="MRNIFFEYNMRGNKKIEQILSNGLIILDTNALLNLYRYNEENKN
                     KFFEILSKVSERIYLTNHSVKEFYKNRSEILYNKAHFKEGMKKTVCEDIKKLRNTIEN
                     VNFKGKFKDSCCLLKYESDLKSQMLKDIDCFISSIENTIDSYNQSNLITDLGETDPIL
                     DKILDLFDGKVNQKFSENELVEIYKEGQKRYKSKTPPGYKDEDDKGEPGCYGDLVIWK
                     EMINISKEKGTDILFVSDDRKEDWCDNFKGKNVGTRKDLIREFFCETGKLFYSFTVAG
                     FIKEISEICDVTDINELEKESKLFESILYETVKETHSSLRRIGVPMTTQSSLKDMQIG
                     GVVKQLKDIQTKLPESAIKQIKDAQIGTPINAMRQNKKLEKTMRLLSNGHK"
     gene            complement(23715..24473)
                     /locus_tag="KPK24_000385"
     CDS             complement(23715..24473)
                     /locus_tag="KPK24_000385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298098.1"
                     /translation="MDSDKIFSKDKLKVHMFYIIVILILALVVVITDRGYDNKDLSQW
                     IAFGATLSGIILSVLAIILTLIGETKSDNTKDSLLNISKKLEDIVGNVEDATYKLENV
                     SDMKDEIKEEISVGLSNMVLKVDKENMPSSLNKDISNKENESNHIEFFREYFKFIRHD
                     NSMKLCILSVFYCGAKQIRHNNGSLGYDDLYSVVKTVLFFDDETMKIAWNVLTVFFSG
                     LNSDDNFYYYICNIFKEIYPYEKECLDLKLEDYL"
     gene            complement(24571..24843)
                     /locus_tag="KPK24_000386"
     CDS             complement(24571..24843)
                     /locus_tag="KPK24_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBG2298099.1"
                     /translation="MKFEEVLPLIKNGEKAFRVGWNGKGMFVVRQKGYPAGIPCNKQT
                     AEVWNLKEGDLFKCNPYLQIKNADNSHSMWVPSTGDIFAEDWEVIR"
     gene            complement(24902..25371)
                     /locus_tag="KPK24_000387"
                     /pseudo
     CDS             complement(24902..25371)
                     /locus_tag="KPK24_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25821..26327)
                     /locus_tag="KPK24_000388"
     CDS             complement(25821..26327)
                     /locus_tag="KPK24_000388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298100.1"
                     /translation="MSKTKKEFFNATKKQLSNYKQLSTNIIKLKNEIQMLKDNSVGDL
                     MKGISYDSVKTGKTNKTSNMIEDAIVNVSDLITEKEIELYEAEIIKSTIDLAIRNLKP
                     IHRQIIELKYIDGLMWQEMVDIVHLEERQLSVRASQAISSISIALFGKKALIEQEPLF
                     ELLDYKLN"
     gene            complement(26432..26608)
                     /locus_tag="KPK24_000389"
     CDS             complement(26432..26608)
                     /locus_tag="KPK24_000389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298101.1"
                     /translation="MKKFIYINRHRVYIRNLFKISAIYKENHYIREFKSECLSNNTLN
                     AVIESSVRIGFGSK"
     gene            complement(26631..27074)
                     /locus_tag="KPK24_000390"
     CDS             complement(26631..27074)
                     /locus_tag="KPK24_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG2298102.1"
                     /translation="MKVNFTIDGEPKAKARPRMSTANGKAYTPDQTILYENWIRLMYN
                     STVKHFFEGNVKMTVICYFDITKKDREALQKNKVNTKAYKNAIDKVEGLIRPNKKPDL
                     DNIIKSVADSLNGIAYKDDSQIVEVVSKKYYSDRPRVEVELEDVS"
     gene            complement(27064..27462)
                     /locus_tag="KPK24_000391"
     CDS             complement(27064..27462)
                     /locus_tag="KPK24_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298103.1"
                     /translation="MQKDVWLYSWDNKYLSSDEYESKEEAIQAAKEELKEFGNFGESI
                     YVGKKEEVSIPNIDIEEALECIQKKIDDEVGECGEDWFENICVEDMIILSNRVNEVFE
                     KWIDEFGYKPYWFKLTDKEEIELNEVANES"
     gene            complement(27616..27846)
                     /locus_tag="KPK24_000392"
     CDS             complement(27616..27846)
                     /locus_tag="KPK24_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBG2298104.1"
                     /translation="MIHELKILPQYFKEVVNGNKNFEVRKNDRGFKKGDLLVLQEFDG
                     EKYTGLEIRKEIIYLLDNSNYLQDGYVVLGIK"
     gene            complement(27869..28141)
                     /locus_tag="KPK24_000393"
     CDS             complement(27869..28141)
                     /locus_tag="KPK24_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298105.1"
                     /translation="MITTQMYKINNEIFLNDLCLEENKEMTIWVEKNKDTHEFIWLKI
                     ANVNGKLAIFIQDIENAVIKEWQGQIAYKKVIYQIEYEQVEKGEID"
     gene            complement(28226..28393)
                     /locus_tag="KPK24_000394"
     CDS             complement(28226..28393)
                     /locus_tag="KPK24_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298106.1"
                     /translation="MTNKEMCKSKNLDEREIYKEFGKEICASCISDRVDCESKDCDIA
                     YENWLEKDAER"
     gene            complement(28402..29406)
                     /locus_tag="KPK24_000395"
     CDS             complement(28402..29406)
                     /locus_tag="KPK24_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG2298107.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDMDLFFQKKIKTVSR
                     AKDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNKSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDEYHLRVLDKDAEKEEANSKFITEFLNATKVKDDKYKTKMFKSTAENWITNAL
                     GNDIKQAEDVRSILNYTLKEKHEIDINNFVDKTIKDDKLKDSFKEHMEEKGLDKGFSI
                     DKKWVEKKLKKRNIKTDNGFDIKGNLTDFEDPMKYTVRQNQNGSIDIIIKNVKFYEEK
                     "
     gene            complement(29403..29624)
                     /locus_tag="KPK24_000396"
     CDS             complement(29403..29624)
                     /locus_tag="KPK24_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298108.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSKEMLKQKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMTEYYCDGGIEI"
     gene            complement(29703..30776)
                     /locus_tag="KPK24_000397"
     CDS             complement(29703..30776)
                     /locus_tag="KPK24_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG2298109.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSGKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGEPMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVVYEIAKRMG"
     gene            complement(30886..31062)
                     /locus_tag="KPK24_000398"
     CDS             complement(30886..31062)
                     /locus_tag="KPK24_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298110.1"
                     /translation="MDDRLEMINASVNYIQMICESSNIAIIAERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
     gene            complement(31165..31614)
                     /locus_tag="KPK24_000399"
     CDS             complement(31165..31614)
                     /locus_tag="KPK24_000399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298111.1"
                     /translation="MSLIKYRGYDFENEKWIYSATIMWSNILECLVMLTEGCKWQKVS
                     NVGVCSGEWARNNQEICEGDILKGYDNSSDTSQYGVVKRDFNSIKLYLEWHYLKKFEG
                     EWIELINKTEIYHSRDYKVIGNEYENLEEVRTEFLERKESLENEYLS"
     gene            complement(31732..32097)
                     /locus_tag="KPK24_000400"
     CDS             complement(31732..32097)
                     /locus_tag="KPK24_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298112.1"
                     /translation="MLKVEKYFNGSVADNIFEDDLTLRNYLALYCCVYGVRKNGELVF
                     PTSEKMLIEFNVDKNRKKKKKGSKVKLINAKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIKEE"
     gene            complement(32111..32362)
                     /locus_tag="KPK24_000401"
     CDS             complement(32111..32362)
                     /locus_tag="KPK24_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298113.1"
                     /translation="MIIIRSQDRLDLMRVNRVEIYDKQVFVIFEDDVKRIGVYESNER
                     AIEVLNRIQEAIIAGTKFDIINKDGVRCNKEKVFEMPVE"
     gene            complement(32360..32608)
                     /locus_tag="KPK24_000402"
                     /pseudo
     CDS             complement(32360..32608)
                     /locus_tag="KPK24_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32682..33089)
                     /locus_tag="KPK24_000403"
     CDS             complement(32682..33089)
                     /locus_tag="KPK24_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2298114.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKAKPVYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            complement(33105..33278)
                     /locus_tag="KPK24_000404"
     CDS             complement(33105..33278)
                     /locus_tag="KPK24_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBG2298115.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            complement(33339..34289)
                     /locus_tag="KPK24_000405"
     CDS             complement(33339..34289)
                     /locus_tag="KPK24_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG2298116.1"
                     /translation="MNFNNETYFHINFDDPFTRVPNTILDNENLSYSAVGVVTQMLRF
                     QRSGSHKVYAKSLISYRKDSKTKVSNALKELMQEGFVIRTQIRDEKGQMKGYRYDIFD
                     TPQNVNSESVETTESQPCAVFPTPVKPEAGKTEVGETGSRQNRGREIGNIKENSIKKK
                     IGLKENDVITTVIAEQSEKNKTVYIKKYYESYIGVITPNNFLQLLTYLDDGMEADVII
                     RAIDEAVGSGVKNYKYVKTILNNWIEAGVKTVLELTEYQNEFERKKKSKQEKKQSNNK
                     AVNTHNVNKNKFANFNQTFTQYEEKELDEIIKKSQKEKFK"
     gene            complement(34298..34888)
                     /locus_tag="KPK24_000406"
     CDS             complement(34298..34888)
                     /locus_tag="KPK24_000406"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG2298117.1"
                     /translation="MEINNIYIKLMDVRVKFSKLNLKKSGENKFANFKYFELADFLPQ
                     ATGLLEEAKLCPIVTFTNEYATLTLINGENPSEQIVFTSPMRDLQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDTFDATSGKNEAKSNSNNRILTDKQLSRLYAIASNANVNK
                     ESLKEKVFKRFGKEIKDLTKQEYDTICNAYEKQNQE"
     gene            complement(34898..35407)
                     /locus_tag="KPK24_000407"
     CDS             complement(34898..35407)
                     /locus_tag="KPK24_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBG2298118.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKKVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            complement(35485..35802)
                     /locus_tag="KPK24_000408"
     CDS             complement(35485..35802)
                     /locus_tag="KPK24_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298119.1"
                     /translation="MKSLTIVRNAVEQQLNRANLEINKNEELYTKLRKKEKRDVLDEI
                     ELSNALREKSVNERLKIFAESLLKIIDTQIEIKEYEESEDYKIFELISEEIERDRPID
                     IQI"
     gene            complement(35805..35951)
                     /locus_tag="KPK24_000409"
     CDS             complement(35805..35951)
                     /locus_tag="KPK24_000409"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298120.1"
                     /translation="MNVRVLIAYVQFCNDKQIKASFEGLRKYNRDIELVNLYLPSKLK
                     IKGD"
     gene            complement(35952..36395)
                     /locus_tag="KPK24_000410"
     CDS             complement(35952..36395)
                     /locus_tag="KPK24_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2298121.1"
                     /translation="MSKLGNISNFNLDKQEDKSFNDLDNISICFSEGIRKVVEMNLNN
                     YKNKISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKN
                     GLLKSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKEIREGVI"
     gene            complement(36425..36622)
                     /locus_tag="KPK24_000411"
     CDS             complement(36425..36622)
                     /locus_tag="KPK24_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298122.1"
                     /translation="MAINDNINKILKDRDLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKIADYLDITLDELVGR"
     gene            36675..36875
                     /locus_tag="KPK24_000412"
     CDS             36675..36875
                     /locus_tag="KPK24_000412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298123.1"
                     /translation="MKVGDIMQFSKDILHTLTLEILKEKYDFKSSSEEELLKHYHEIF
                     LKLSEVNNSFSKGDGLSVFKQM"
     gene            complement(36862..37059)
                     /locus_tag="KPK24_000413"
     CDS             complement(36862..37059)
                     /locus_tag="KPK24_000413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298124.1"
                     /translation="MKISFESVVNNVNNETELILSKEELQIAKRILNTLNENEQSILS
                     SKDILDFCKEALKYNLVPTFV"
     gene            complement(37119..37910)
                     /locus_tag="KPK24_000414"
     CDS             complement(37119..37910)
                     /locus_tag="KPK24_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBG2298125.1"
                     /translation="MNNLQIFEKLEFGQIRMVEVDKKPYFVATDIAKCLGYANTSKAI
                     NDHCRWVTKSYIPHPQNENKILEVNTIPESDMYRLIVNSKLPNAEKFESWVFDEVLPT
                     IRKTGGYIHTTEDMTDDEIMARALQVAQKTIEKKSREIEEKDKVIQLQQPKVLFADAV
                     ASSDDSILVGELAKLLKQNGIDTGEKRLFAWLRDNGYLIKRKGEDYNTPTQKSVDLKI
                     METKKRAIYNPDGSTKITKTPKVTGKGQIYFINKLKLVNQLSMLS"
     gene            complement(37913..38083)
                     /locus_tag="KPK24_000415"
     CDS             complement(37913..38083)
                     /locus_tag="KPK24_000415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298126.1"
                     /translation="MEKVRMTVRLVPKLNQYVQNIAKECGKSKNSIIVDACWEFIEKI
                     KKENKFNVESEE"
     gene            38204..38515
                     /locus_tag="KPK24_000416"
     CDS             38204..38515
                     /locus_tag="KPK24_000416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298127.1"
                     /translation="MTTITVRIYTPLNENLEKISYQTGILKSSLILYAINDIIRNSKV
                     NELQSISYKSDDTVRSTLRIPGVLKELLEKTAKENNLSVNSLINNAVHSFCISHWLIY
                     L"
     gene            complement(38501..38638)
                     /locus_tag="KPK24_000417"
     CDS             complement(38501..38638)
                     /locus_tag="KPK24_000417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBG2298128.1"
                     /translation="MKHEQTTLRIPEDLYKALIDLSKEIGMPIASIVIIACWLYISKI
                     N"
     gene            complement(38693..39481)
                     /locus_tag="KPK24_000418"
     CDS             complement(38693..39481)
                     /locus_tag="KPK24_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBG2298129.1"
                     /translation="MKNLTIIKQNNQFLVESREVAELIEKKHDNLLRDIRGYKKILED
                     SSNLRSQDFFIESTYINTQNKIQPCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MEQELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADSVA
                     SSDNSILVGELAKLLRQNGIDTGQNRLFDWLRNNGYLIKRKGEDYNTPTQKSVDLGVI
                     ETKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKSSKQLSMLS"
     gene            complement(39450..39713)
                     /locus_tag="KPK24_000419"
     CDS             complement(39450..39713)
                     /locus_tag="KPK24_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2298130.1"
                     /translation="MYLNRLEGLMKENRHTQKNVADILGLSSYGFRLKLKGKNEFKAS
                     EIKKISKLYNVSADYFFSDEVAKIAIKEERGNNNEESNHNQAK"
     gene            39892..40287
                     /locus_tag="KPK24_000420"
     CDS             39892..40287
                     /locus_tag="KPK24_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298131.1"
                     /translation="MNRIKELREEKGISLDKLSEDLHINKSTLSRIENGLREPKKSTI
                     EEYANYFDVSTDYLLGRTDVRNSLFINKNEKDYDAENFKTEKELIENMYLDEDMKEVF
                     NIFSELSPDAREKALKVAELFLLDEKNKK"
     gene            complement(40324..40461)
                     /locus_tag="KPK24_000421"
     CDS             complement(40324..40461)
                     /locus_tag="KPK24_000421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298132.1"
                     /translation="MNKTKYYDILKLNLLMKKLKELDKNKINEYKIKVKEIHKINKKE
                     E"
     gene            40745..40966
                     /locus_tag="KPK24_000422"
     CDS             40745..40966
                     /locus_tag="KPK24_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298133.1"
                     /translation="MLKELRKKKKLTQIELAKRVGCHRSQISRLENNEDKDLTISILI
                     ELEIALGLEEKYLVNYFADEYIKKRKLHK"
     gene            41025..41231
                     /locus_tag="KPK24_000423"
     CDS             41025..41231
                     /locus_tag="KPK24_000423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298134.1"
                     /translation="MNLSFNKKKKFELSKEELELIENWLGNKYAGDMTIPAIVDFSLE
                     TDIEYEKIVVYLAEKVLESRDKKH"
     gene            41368..42534
                     /locus_tag="KPK24_000424"
     CDS             41368..42534
                     /locus_tag="KPK24_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2298135.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVERVGGNTKKEAEKSLR
                     DAINEYENAGIVFDETNMSLSDYLNFWYKEYVLLNCKYNTQESYRNLIENHIEPRLGK
                     CKLKSINPAIIQEFLNNKSKETYTQNGEEKHYTKGVLKAIYVVLNAALKSAVYPYKLI
                     KENPVQYASIPKNVLKVKNESDNKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRK
                     GEILGLCWDNVDLDNNIIKVRKNLIKRKVSEFELASPKTKTSIRDIKIGDTLSRILKE
                     EKLNQKKQKIKIGKWYKETEYDWVCRKKDGSFVNHNNIDACVRTINKKLNLDFNFHCL
                     RHTHATLLLENGANIKYIQQRLGHSQLSTTMDTYSHVTSKMESETVDILEGILQ"
     gene            complement(42444..42828)
                     /locus_tag="KPK24_000425"
                     /pseudo
     CDS             complement(42444..42828)
                     /locus_tag="KPK24_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(43068..44462)
                     /locus_tag="KPK24_000426"
     CDS             complement(43068..44462)
                     /locus_tag="KPK24_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG2298136.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(44482..44691)
                     /locus_tag="KPK24_000427"
     CDS             complement(44482..44691)
                     /locus_tag="KPK24_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG2298137.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(44720..46165)
                     /locus_tag="KPK24_000428"
     CDS             complement(44720..46165)
                     /locus_tag="KPK24_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2298138.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            46275..47069
                     /locus_tag="KPK24_000429"
     CDS             46275..47069
                     /locus_tag="KPK24_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG2298139.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            47120..48442
                     /locus_tag="KPK24_000430"
     CDS             47120..48442
                     /locus_tag="KPK24_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG2298140.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(48542..49759)
                     /gene="cdeC"
                     /locus_tag="KPK24_000431"
     CDS             complement(48542..49759)
                     /gene="cdeC"
                     /locus_tag="KPK24_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG2298141.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(49942..50122)
                     /gene="ssrS"
                     /locus_tag="KPK24_000432"
     ncRNA           complement(49942..50122)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KPK24_000432"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(50237..51142)
                     /locus_tag="KPK24_000433"
     CDS             complement(50237..51142)
                     /locus_tag="KPK24_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298142.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            51354..51752
                     /locus_tag="KPK24_000434"
     CDS             51354..51752
                     /locus_tag="KPK24_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298143.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(51875..52891)
                     /gene="ccpA"
                     /locus_tag="KPK24_000435"
     CDS             complement(51875..52891)
                     /gene="ccpA"
                     /locus_tag="KPK24_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG2298144.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(53144..53344)
                     /locus_tag="KPK24_000436"
     CDS             complement(53144..53344)
                     /locus_tag="KPK24_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298145.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(53378..53557)
                     /locus_tag="KPK24_000437"
     CDS             complement(53378..53557)
                     /locus_tag="KPK24_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298146.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(53739..53945)
                     /locus_tag="KPK24_000438"
     CDS             complement(53739..53945)
                     /locus_tag="KPK24_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298147.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(53993..54418)
                     /locus_tag="KPK24_000439"
     CDS             complement(53993..54418)
                     /locus_tag="KPK24_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298148.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            54590..54817
                     /gene="acpP"
                     /locus_tag="KPK24_000440"
     CDS             54590..54817
                     /gene="acpP"
                     /locus_tag="KPK24_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG2298149.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(54961..55965)
                     /locus_tag="KPK24_000441"
     CDS             complement(54961..55965)
                     /locus_tag="KPK24_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG2298150.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(55979..56881)
                     /locus_tag="KPK24_000442"
     CDS             complement(55979..56881)
                     /locus_tag="KPK24_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG2298151.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEDLINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(57077..58252)
                     /locus_tag="KPK24_000443"
     CDS             complement(57077..58252)
                     /locus_tag="KPK24_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG2298152.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(58339..59184)
                     /locus_tag="KPK24_000444"
     CDS             complement(58339..59184)
                     /locus_tag="KPK24_000444"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG2298153.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(59282..60079)
                     /locus_tag="KPK24_000445"
     CDS             complement(59282..60079)
                     /locus_tag="KPK24_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG2298154.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(60098..61108)
                     /locus_tag="KPK24_000446"
     CDS             complement(60098..61108)
                     /locus_tag="KPK24_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG2298155.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(61127..61909)
                     /locus_tag="KPK24_000447"
     CDS             complement(61127..61909)
                     /locus_tag="KPK24_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2298156.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(61968..63104)
                     /locus_tag="KPK24_000448"
     CDS             complement(61968..63104)
                     /locus_tag="KPK24_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG2298157.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(63813..64280)
                     /locus_tag="KPK24_000449"
     CDS             complement(63813..64280)
                     /locus_tag="KPK24_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG2298158.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(64359..64901)
                     /locus_tag="KPK24_000450"
     CDS             complement(64359..64901)
                     /locus_tag="KPK24_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298159.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
BASE COUNT        19771 a        11884 c         6858 g        26443 t
ORIGIN      
        1 tacaatctat ttgaaactag agtggagttc gtaatttaaa caattaataa ttatttaaat
       61 ctacggaatt tgattttaaa atcaaactcc caaccactct tagcggctac tttgagtggt
      121 tttttacgtt ttttaagtaa attgctaact agacaaacta ttaaactaac agatagcctt
      181 tctagtacat tttgtaaaaa attatccata catactcacc tcccttctat acgttgggag
      241 gataatcttt tgtatgaact ccactctata aattgtagat tacatcttct tgctacaatt
      301 attatatcat ataattctta catattttac ctgtttatta ctttttacgt tatagtcacc
      361 tactttcaac aaaaaaacac ttacctaagt aagtgttttt aatatattat ctaaaatatt
      421 ttcattttct tgttgttcta taagaatttc tcttaatatc tttacatatt cttgaaactt
      481 ttcttcacta ttctgtttta gttcatataa cgcattagca aatttcacaa aatattctac
      541 atcttcatca gtttttaaat tatatttatt aagtaaactt tcacgcattg tattaatccc
      601 cctcaaaact aaaataaact aaactaaatt atttaataca actgataaat ttactcaatc
      661 ttataaacca catgataatt cttcttctca cctgcaattt tgacaggtct attattttcc
      721 tctatccaag ttctcacctt atctattaca ctctttgtat atttatttac agtaccagtc
      781 caagaaccat tcgtttccca aactcctttt acttcatttt cttctaaatc aatcttttta
      841 ataatttcac aaacagccat ctgagctggt ttattactct tagaatatat tttcagttta
      901 gacgctattt gttttgtatc aaaataatgt tcttcttcgt ttatctcaat cggtaaatca
      961 atacctgcct ttttatataa tgttttagct gttaatagtt ttgatttgtt gtcaaatcct
     1021 gcatcatcca atagttcttt taacatagat gtactattat aagctaattg taacttttca
     1081 atctcacttg ctttttctct tagtttttcg ggattagcat tattagttat gtatgcacca
     1141 gtttgtcgaa tagctggaag tacttcatcg cttatccagt cttggaactt ctctgcttct
     1201 tctttctgag atttaaatat taacttgtat accccactct cagttaagaa cttttcacct
     1261 gcattattca gttttcggat gtccttatct aggacagctg aattttttaa caagatagct
     1321 tgagtatcat tcattttaga taaatgattt cttattgcac tatcactcaa ttctaagcat
     1381 cttccgcagt cgtacggatt aaataaaact tgcccattat attcaagtac ttcaactttc
     1441 ttttcttcaa acatcattaa ttcatttttc ataatattac actccttaaa attgattttt
     1501 ttcaaggaat gacgtatact atagttagtg tatataatta tacgtcaata agggatgttc
     1561 aatctttggt cggggagaac gtcccttatt tttattcctc tttttctagc tcttcattaa
     1621 tcttttcttc aagccaaata gttttagtct tgttctgctt ttttaaatgt tcttcaattt
     1681 tctctacttt ctctctatct agtagaacac taaaagtttt tttattctgt cgtctttgct
     1741 tgaagtaatc tgctctactg ctatcagtaa taattttcac ctcttttctg tatcgcgata
     1801 atataattat acattgtatc gcgacacttt tcaagagttt ttactaattt tttctaatta
     1861 ttttacccaa ccgaccaaat ttgagcaaaa caaaagcacc taccaaaagt aagtgctttc
     1921 tttttttatt tatttttttc cacatagtta atataaaaca attatacaaa gatacatcta
     1981 atactaagat tgataaattt acataccact cagttaatat aaaatcctgt aattttgttt
     2041 ttatcaagta atgttttagc atttacatac cacatagtta atataaaatc ttgagcaaat
     2101 atacttctta cagtctctaa agccttattt acataccaca tagttaatat aaaattgtat
     2161 ttgaaggagg aataaaaatt gtttcttctg ttatttacat accacatagt taatataaaa
     2221 ttcagacata gaaagaatgg gtgaaacttt taagtataat ttacatacca catagttaat
     2281 ataaaatttt ggaatgttcc ttttaattct gatactgaaa tttatttaca taccacatag
     2341 ttaatataaa atcttatact ttgttgcatc ttataccccc agataaagat ttacatacca
     2401 catagttaat ataaaattct gataatagaa tgtaaagaat ttattttaag cttatttaca
     2461 taccacatag ttaatataaa atcccaaaat aaatttagta tttccaatac ttacatatat
     2521 atagctttct taaatttgca gtgagcgacc agtagtgttt tttggcattt cataaagcat
     2581 acctgtaata tagtaatttc aatcactaca agcaatattt caaaaaatcg aacactgcta
     2641 aagccttacc tatattatac cattttttaa catataaagc acttgaaaca acagaatatc
     2701 caagtgcttt gtatgtttat acttatttat tttctcttgt tataaattca ataattcttt
     2761 tttcttctta ttaaattctt cctctgttat tgctcccatg tctaataact cttttaaccc
     2821 tttcacttgt tgtattgcat catcatttga ttttatttgt ttttctttat tattttctaa
     2881 attattctta cttatattta tttttctcct tatattatca atgaattctt ggctatgaaa
     2941 attaagtatt gaatcttcag ctattatttc ccttaattct ccatgagaaa aatattcaat
     3001 acttataaac aacttttcag ttttactttt tttatttcca gttcctgaca acccaccaac
     3061 aattgttccc ataggaccaa ataaagttcc tatagctgct cttccaacaa cagacttatt
     3121 attatatgct atttctttct cactatactt atttacacta tataaatcag aaaatttaat
     3181 acgcattttt tcttctttta aaagtttgct ctcaataact aaacatgcat tatggttgtc
     3241 aataattata cttattgctt cacccctaca attaggaact ccaacagaat tatagtaatc
     3301 catcttcatt accccttgtt taactatttt atttttattt cttaatatag tatctgtctt
     3361 tttatcttgc atatctgcta tattttgatt aattatattg catattttat cttttgagtt
     3421 acttttaaaa tacaatacac tttttttgct ttctataatt agcatatcat tatcaataaa
     3481 gactttctct atatctaaaa gttttatttc actaataggt acttgttgca atgtatatac
     3541 agatactagt tttttatcta tatacatagt acatgtaagt tcattgttaa aatgtgggtg
     3601 cccttttata tacataagct taaaattctt tatgtcatct tttcgtttaa aaaaattcat
     3661 tttaatcccc tcataaatca atagtctata atgttattat aacatctata aggaggattt
     3721 ttttaacaat aattcgacat tatccaatat cttgtaatgc ttctcttagc tcactttcca
     3781 cttgagacaa tatttcttgt atcatttctt ctttgttatt gctaccttga atatttatag
     3841 atattccacc aacattaatc gcattacttc caccagaaat tatgttttgt ggctgggctt
     3901 cttgataaat tctattttct gtattattaa attcttcttg tttggtagga aattgtctaa
     3961 cattattaat aatattagaa gtactatttt gaatactatt tgtagaatta aaactagttc
     4021 ctaatttttg agatattgga ataacattat tacttgcttt agttccaagc atctgtcctg
     4081 cttgtttata caaacttaat gctctacttc ttttactatt agaaagagga ataaccattt
     4141 ctggacctgc ttctccacaa atacttggtt tacttgcaac accaccatct gcaaaatggt
     4201 ctagtatatt gcttactcca gtttttacta tacttacaaa accagttatt ttggtagaaa
     4261 gtttcttttt aagagaatcc caagcagatt taattgaatc cactttactt ttaaatccgt
     4321 tttctacaag acttacaaat ccacttattt tgccagataa atttatttta agtccttgcc
     4381 accacattcc aacttgttga actttttgtt gaaaaccatt gcttacaaaa ctaacaaatc
     4441 cgcttatttt ttgacctaca ttagttttta aatcagacca ccattgtttt acttgcccta
     4501 ctttttctga aaatccatta tcaataaaat ttacaactgc tttaagtggt gctcctaaaa
     4561 ctccttttat accttcccat aatgatttaa ctacatctcc aatacctttg aaagcatcag
     4621 aaaatccttg ttttattttt tcgccatcac cactaattat cccacctatg atttcgaata
     4681 ttccttttat tatatcaatt acacctttta tagcacctgc tatagcattt ataatagatg
     4741 caattgcatt aataacagaa gttataacta aaactataga agttgctacg cctttaagta
     4801 atcctcctcc tatatctccc agagtagatg ttaaagaatc ttttatctgt tttaaataat
     4861 ctacaaaagg ttttgctgct tcttttaatt gattaaaagc attccctagc tctttgaaag
     4921 acgttcctac gctttgtgtt gattgtttta actcatccat attggtttta gttgtcttag
     4981 ttgctccatc atctttgatt ggcttaaaca aatttgaaaa gaaatctttt attccactaa
     5041 atgcttcttt tattggttca agagcttttc ctagttctgc aaagctagat tttaagttat
     5101 caaaaactgg ttttaaactt tcttttgttt ctaaaacttt ttgttttaaa ttttcaaaag
     5161 gcgttttaat attttcatca aatacagttt ttaaacctcc aaaagcttct tttatgctat
     5221 ctaaagaatc tccaaaaact tcttttaaat tacttaatga ttgtttaaat gtatctatag
     5281 caggttttat gccttctaaa agtttatctt ttaattcagt tgctttgcca cctataaaag
     5341 ttactatatt attaaatact tctgtcgcag atgttttgag ttctccaaat ttctctttaa
     5401 tctttccaat accttcacct attttttgac ctaatgagtt tatatatgct tttgctccat
     5461 tcgatgaagt ttgcagttcg cttgatgctt tctcgctaga taaattaaca ggctgaactt
     5521 ttggagctgc tttagcagga ttttttaaaa agtcttttaa ttcattccat tttttcttta
     5581 tgccttccac tttttttccg aatttttcgt ctagaatatc tacaacagct tgaattggtg
     5641 atgtcacaaa atctactaac ccactccaca atgacttaac aatatctata atccctttaa
     5701 aaatagattt aagcccgttg actacttggc tcatatctct atttataaaa cctttaacta
     5761 catcagctat ccctttaaat atctctatta aaccatttac tactactgtt gttgtattta
     5821 ctatagcttt taccttgtta acaattacat taaatgaata cataaatttt attatgaaaa
     5881 ctgtcgccaa aaattgtata actggtgata aagcacttac aatcattgag cctaatttag
     5941 aaaaaactgc aaacaaaggt tttaacgcat ttataagttc tttaaattta ccttttattt
     6001 gttctataaa aggatttaaa ggttttaaaa aattagatat tgatttacct atgtttttaa
     6061 taccatacct aaaagtttca gatttttgat aagcaaataa aaatgctcct gctaatacgc
     6121 ctatagctaa aactattgca ccaacgggtc caagaactcc aactattcct ccaattagcg
     6181 ttgatgctgt agctatcttt gtaattatac ttattacgct agaaattact gttaaagctt
     6241 taaaagccat aaatccagca ataacaccac caacaacaga agccacacct tgtaaaacat
     6301 tttttatttt atcaaagtta tttataaaat tactcacaaa atttacaatt gaatcaccaa
     6361 ctttaggcat actactaatt atattttcca taaagtttct tgttactgga cctaattttt
     6421 ctcctatact aatttttaca tcatttatag tgttttttaa tttagcgaac tgcccattca
     6481 aggaatccat cttcatgtca gctattcttt tagcttcttc ctcgctttca gctatggctt
     6541 tttttagttt attgtagtca ctttcactag catttactat tgcagcccaa ccacttgaag
     6601 cgttagctcc tactatatct cctaaagctc ctactttctt cgtgtcacta agcttaccaa
     6661 gcttatttct aagttcatct attgttgctg ctaaatctaa acttccactt ttagttgttt
     6721 tcatttctat tccatatttt ctcatagctg ttgcggctcg tttaggttca tttataagtc
     6781 ttaaaagacc cattcttaga gaagttcctg cttggcttcc cttgatagca ctacttgcca
     6841 tcaaacctgt agctaatgaa aggtccttca tcggtacacc caaagaacca cctaaggaac
     6901 caacaaattt caatgtttcc aattttgtta tcgtaaaggc tctttatccc ttgcttctat
     6961 atatttctat atagttcaga ctatatctta agttcaactt aaattgaact tgttgctgtt
     7021 cgtggatatt tctgcatata aaaaggcact tcatgagtgc cttatactta gcttacttta
     7081 tctagtcgtt acacgttcca gttgtttcca aactggcttc gctcggtatt cccatattga
     7141 ttttttcaaa tccatcttct aattttttta taattttatt ttttataata tttaaatttt
     7201 cacttctaac atattttatt cttataagag gtatattatt ttttttacaa aactcatttt
     7261 ttatttcatc attatatttt attttttgaa aatcttcttt actacaatta aattttggca
     7321 taaagtgttg agaaccatca tactctatta aacacttgat ttcacttttt ttaaaaatag
     7381 caaaatcaaa aggcaaaggc cttttattct tgcattcttt aattttatat tgttccttaa
     7441 aatcaaattt attttcgttc agatattctc ttatcacttc ttctcctttt gacctgtaac
     7501 aatgtgggca tcttattcct ccatttataa aacgtgttgg ctccatataa aaataatttc
     7561 cacatgatac atgaaaaaat ttagcttttt tactggcacc tctgtactca cctataatct
     7621 tataatctcc tttacccaat tgttccacat tttctttgat tatattaaaa ttaattgtat
     7681 ttgaatttgc acttttttta tatctttcat atggacatct ttgacctttt aaaaaatctg
     7741 ttggtctcat tttaaaagtt ttaccacata aattatgttt aaataaaata ggtgttgatg
     7801 aatttatata ttctcctata atttcatact cagttcctga taaacttgaa atttccgatt
     7861 taaatttatc tgttgtcttt ctcaagttat ttgaacacaa tgggcaacaa ctaccactcc
     7921 acaagctttc aggatttgct tcccactcat aaccacattt atgttttacc aatacttttg
     7981 ttcttttgtt tttatatatt ccaagcactt taatttctgt tttatgaatt tttcttattc
     8041 tttctataaa ttcttcgttg gtaagttttt ttgccatata atcacctctt aaaataatta
     8101 tacgttatta atttttaagt tgtaaacaaa atctaactta gggttcaccg aattaagcaa
     8161 cttttaaatg ggcaatacat tcacccattt tttcaatatc agtattagaa tttgttatag
     8221 ttgcagccat aatatcaaca aattcattag tatctttggc agccattccc aatgatgtta
     8281 atccatccgt cactatatca gacgttagtg ccaaatctgt tcctcctgtt gctgctaaat
     8341 tcaagatgcc tggcatacct tcgataatct gattagtttt ccaacctgcc atacccgcat
     8401 aatacatggc atcgctcact tctctagctg aatatgcagt tgttttccca agatttctag
     8461 cttttacagt taaagcttcc atttcttttc cgcttgctcc tgtgacagct tgaacgtttt
     8521 tcattccttg ctcgaaatta gcaaaagttt ttatcgaaga accaacccct atacctccaa
     8581 gtgcaacagt aattgctgtt gttaattgag cgaatttact tattgctcca cttataaatg
     8641 aatctatttt acttccaata ccactcaaag ttggactagc ttcatctttt agttttacaa
     8701 tcgcttgata agtcttatta gaaaactctt gtaatttact cttagtacga gaaatagtat
     8761 ttaatgcttc ttcacctttt gctttaatat ttattattgt attattcttg agctctccta
     8821 atttacttct agtttgagaa ataactctta gcgctgggtc agctttcata ttcaagctaa
     8881 ttattgtagc cgcagtcaaa ttttgtacct tagcttttac tttatctaca acttgactag
     8941 ctttgtctct agcctttaat aaaacttctc tttgtcgact tgtaagtaag ctatttactt
     9001 tgtttttaac tctatttaca acgctagatg ctctatcttt tgcgtttatg gttgtagata
     9061 tagttctacc cactcttttt aagttgttgc taattctatt tacaacacta gatgttttat
     9121 cttgagtttg tataattgga ttagctttta tcctattcag tactttcatt ctcttttctg
     9181 tctgtttcat gtatctttcc atagcactta atttactctt agtttgttca tctcctgtaa
     9241 catcaataac gacatcaata tgatacattt cttttttagc tatttctctc acctcacttt
     9301 tagtttaaat tttatttatt tttcatagct ttattttctt gttctatttc atattgagta
     9361 aaaactctaa gaagctgttg aggtgttttc tctcttttta gaaaatcttc tggaagaaca
     9421 ctatgtttaa catatgcgtt atataaaata gtaatcttcc cacctctctt tattagtttt
     9481 ttatatcacc atcacttaat tcttcataaa atccagacaa ttctagtact tcatcactaa
     9541 ttaatgcaat ttcgcctgct aaaaactttc ttcttataaa ttccactcca tttgatacct
     9601 tcatagaatt aagaagtctt gtatccccaa aattaggaac tattgtagct ttttctatta
     9661 gagctatatt aaattcatct tccattagct tactttctct tctacctctt accttagtaa
     9721 cttttgtata ttttttctgt aatgcactta tctctttttc tgttaaagcc ctcagtgtaa
     9781 gtggtatatc taaccttttt acaaaaatag ttttttcggg taatatagca tcctctgtca
     9841 atttcataat tatattatct tcttgttgct ttgctatttc atctttagta agctctcttt
     9901 cttcttctat tccttcattt aaaaattctt tatctaagtt tcccattttt aacttcctcc
     9961 aattttattt ttatttttca taaaaaaaga tatatacttt tggtatatat ctcaaaattt
    10021 ctttaattca ttttttgtaa tagatggttt aaaaactaat aataacttta taattaatat
    10081 ttccacccat gttttgttgc catttctttc aattgctcta ttttcttact aacatcttca
    10141 tcattagaga aattaatact tctcttttca aaatttacat aacattcatt atctttaaag
    10201 ttataaataa cctctccaac tttaagcgag tctttaaatg tatcatgatt cgaaatgttt
    10261 atttcatctc ttacatattc gcctatacgt ggtattatgt cagtgtctac ttgctttgtt
    10321 atgataagtt cttctctttt cccaaacata ttatgcatac tcatcttttg acttaaaata
    10381 actttcattt tattacctcc ataatatatt gtaattgttt ctttatataa ttatatcatt
    10441 aactatatca acgttctaca tattacgagt atataatatt tgttttttat ttcataataa
    10501 ttaatctata aaatccaaaa gttcaaatcc ttcaaaagtt ccatctattt gtatttctat
    10561 attttcatca gattttatgc ttgctagttg tattttatct accatacaat ttttatatct
    10621 aattctttca tatccaacta atccaggatt ttctatttct gtaattattt caaatttatt
    10681 aaatcctttt ttaatccact tagatgtagt tttaagtaca gtcaaggaaa aagttccttt
    10741 ttgagtggat gccttgttaa gttcccattt acaaccaatt actctaaaag tctttttatc
    10801 attttctacc tcagctgtaa attctgttcc atatccttct tcttctccat caattattat
    10861 tctagcattt gaaccatcaa caacatttgc agcatctata atattttcat catattttcc
    10921 catattttat aacctccttt atcctaggta tccagtacca tatattttct tcatcacatc
    10981 aactttaata gcatcccact tccaataaaa ttcatcagct ttggcagttg cttgaagttc
    11041 tgtatctata tcaacattaa attcagatat aataccttga ctcatcaatt cttcaaaata
    11101 tttcttcaat gcacatataa cagttgtttg acctgttgaa tcgttaaata tcttacctac
    11161 aaattccttt cgcttcaatg aagtatcttt atttatagta ttaataaaca tgatattaga
    11221 gatatatccc attgcttcat ttttatcatc tacatatttt ttaaatgtgt tcacatcatc
    11281 aactataatc acgtctccat catcaaaatc taagaccaat gtaccacttt tcaagcattc
    11341 tttaacttca ctttggctta atcgtggttc tacttcttca aatatagttt ttgcattgca
    11401 tatacttcct gttatacctt tgcttacaga aagagcagca atgtaaacag ctacttcact
    11461 aggtgtatat tttacatttt cataataagc tgaactccca acattaacta tattttcatc
    11521 attgaaacct tttgatttat catttatctg ttttatatta tcctcttttt tcccacctag
    11581 aaataataat atatcttttc ctagctcctt atttttagtt acccaagctt ttgttgtttc
    11641 ttgcaatgct tcatcagcta caccatcaag tacaaaagag tcaaagctat atctttcaaa
    11701 ctcctccaaa gcttttaaat aagactcatt agtaatagat gtgcaaccat cattaccacc
    11761 ttcaagtgct acatttacta agtttgctag tgttgtatca ctatcagcta tttttgttgc
    11821 aattacatat tcattatcta aatttgaatt tatttctagt actatttcat ctatagtacc
    11881 tttaacactt gaactaaata attgtttagt tccttcaaag aatataaagt cttttttatc
    11941 tgcatctact agattagatt ttattgttac attaaaattt ctgctagttg gatatttagt
    12001 ttctagctta attacatctt ttgcagtatt ttctgtagta tcttttagtg ttaatgtacc
    12061 cttcttttga tttccatcta caagcctata taaaagtaat tcctttacat tccctaataa
    12121 agctaattta cctaacttat atgctgaata gttcatatca tcaccaaata aagttttaag
    12181 ctgtctcaag tcatttttta ttgttacaac cttgccaact tctccccaat tagccttaac
    12241 tggtattgct aatctaccct ttaatccttt atttgtagac ttttctgctt gagtcttgaa
    12301 cctgttataa aaaccaggta tctccttttt ttctttttca ttccatgtac cagttgccat
    12361 tttacttcac ctctctttct aagaaatctt ttataagctt ctcaaactct gattttgtaa
    12421 gttcatcttt cttacaatta aataaagcac ctgcaactac ttctttcttg tagccaagtg
    12481 cttcgctatt ttttataaaa tcacttttca aatatttttc ttcctgctta cttacattag
    12541 tctttttatt aattgtttca gccaaccctt gcacctccta tcttaagttt ccattactat
    12601 aaatcttatt cataataggt ccttctcttt ttatcttacc tatcatttta aatactacag
    12661 ttaattgacc attagaaaac atgtccgatt ctctatcttc aactacactt acaagagtta
    12721 aatacatgtt tttatcttct ctaagtctta ctctcttatc tattattagc ttagtctcta
    12781 attcttcaag aagcttaact atttcatcct tatttttgct aacaacatga catttcatag
    12841 ttttagtaat ctcaatcaga tgatagttaa tccttttgtt ttcaatattt gtagttcgcc
    12901 acaaacagca tggcgctata aagtttttct tccaattatc cttgtaactc tcgatttcta
    12961 ataaatcttt tgtgtactta gatagagctt caacccatct atcattagtt atatcctctt
    13021 tatcatctaa agctattaca ctaaacctta gacatcttat tatagcttcc cattcctcgt
    13081 ctataacatc ttgaccaact gcaccctcat aaatacaagt aaatacctcg ttagatgtat
    13141 tatctgttat agttttaaaa tctaaagttt ctatgacttc tttcgtaagt tcatccaact
    13201 tattaaatgt agtccttttc tcatatagcc aaatatttat ggtccttcta aaacctataa
    13261 catcaccctc gttgtcagca tcttcacctt gaacaattac agcatatggt tttatagtct
    13321 ttttattagg tacagttggt tcataacaat ctttaagctt tggtatgttc tcaattaagg
    13381 cttttcttat tcctgctctc atattattta ctccaatgtc cttctattaa tttccctatc
    13441 ttaggcatat tcctactaat tgtggactct aaagaatgtg tacctttagt accaggatgt
    13501 tgaaccttca taacaggatg tgaagctcca ttccaaaaca aagcttttgc atttcttggt
    13561 tttataatat gaggtgctga accttcctct aaaacagttc catagtcaac accatgacct
    13621 aacctaacaa tgtattgatt tcctccacct aagctagttc ctgttatacc ttgtcttgca
    13681 tttcctgtcc tatcagtcca ttttgcacta tttttagctt ctccttctaa cataaaagct
    13741 atattcatac acagaagtgg cattgtagcc ttttttctat caatttcatt tatagcctta
    13801 gtaaacacac tcatactaaa ccacctctaa tctagctttt caagaccaca tatataacca
    13861 cagattttgc cttcaactac aacaggattt acatagttta atttcatagt accttcaata
    13921 catttaaatg ttatattact ttcagtattc aatcttaaat cagcttcttt atctgtaacc
    13981 ataccaaagt ttttattttt ataagctgtg ccaatagttt cactatttat tactgtatca
    14041 ttagtttttt cgggatatat aactactgtt aattctttta cttcatttgt aacctcaata
    14101 gccccatcta ctatattttt cacttcttgc tctatagtta ttgtttgagg atttaaagct
    14161 attcctctat tgatagtctt tattatctta tcagctctta attttctcat tgcccatcaa
    14221 ctcttatcat agatgtttta tatcctgtgt tagttgtttc attctttgat ttttcttcta
    14281 aatagtcagt tttatatata tctgctaatg ataaccagta tgaactatta ctactcttag
    14341 tttctatagg gcctatttta atacaatcat ctgtagcacc tttgagtaaa caacctctcc
    14401 atgaagcttt taaaacatta ttctcattag attctaataa catcacaagt tgttcatctg
    14461 taaaataagg atactcttct tcttgcaaat taagttttaa tttatctaaa ttggtaatag
    14521 acatatctac tcaccatctt tttcaagaag ctcattatca ctattattct tttcttcaat
    14581 ttctcctatg acttctatat atcctttttc ttccatagat tcttggtctg cttttctaat
    14641 ctcaaataca tcacctattt tatagcatcc attatcatac tttagatata ccaaagcttt
    14701 tacttgcatt aaattatctt tctttttagc cataattatc acccccttaa ttatgctact
    14761 gtagcgaaga aacattcatc agcacgttca aagcttggca tacctagttg tgatacctta
    14821 gtttgaactg ttactgggtc aattaatctc attgttgtta tagcaatacc agttctaaca
    14881 acagaacaat ctaatttaga accatatact ttgtcagctt cttctggtgt tgtaccataa
    14941 taagtttttc ctaaatctcc atctggaata aaagttattt tgttatcagg gaaataagat
    15001 tcttcacttt catcttctaa tttatatgtt ccacttacta tggcaacaga taaaccaact
    15061 ttatttttaa gataattttt aatcatttca tcagttaaaa taactctacc atctttatct
    15121 atatctaatt taatagcttt atttttagca aaatacccaa aggttttact tgtcattacc
    15181 attctcttag gtaacggatt cccttcatct ctcattattc tcatccatct ttgaatatct
    15241 cctataatat ctgcatctgg attatcccat gttgcacttc cagttaacac ttctttatga
    15301 ttgcttggaa cttcaaagtc aaatactaaa tctccatctt ctgatacgat atttattact
    15361 ccatcagcta gcgcctgcat tctcattctt tccatttgca tatcgccacc atctacaaga
    15421 gctaaataat tatcatatat ttgtgaaatt atcattagta acagttcttt attttgagct
    15481 tgtgaagcta aaagcaattg ttgtctgtct tcctcattta caagaacact ctctttaaaa
    15541 aatggcattc tctttgattt aacttctatt tgagctttta atgctcttat ttttacagca
    15601 acatcaaaag tactttgttt taacactact ggttttttct tcgctccctt aatatatttt
    15661 aggtccatac ctatttgctt ttttcttgga aataaagctt cgcctattaa catttctagt
    15721 ggtaattttt ttatatattt agctatctcc ttagaatcta taaagtcttt ccaatccatt
    15781 ttaattcctc ctaatattta ttttataaaa acataatcat tttcattgct tgttttgctt
    15841 cttctggtat agctgttggc aaggtctttt catctataaa tccaaatata aatactggaa
    15901 tactttcatt cccatttgaa taagtaaaat ctatatctct atatactaaa ccaaaagcct
    15961 tatcatttgt aacagttgta ccatctacca attttccatc ttttgatatt aatgtacctg
    16021 cttttaaaat tctcttttca tctactatag ctacatcggt ttttttcact ttaatattta
    16081 cattttgaaa taaatttcct gcaaatttta atatggtttt attttccccc atgtaaatct
    16141 cagatttttc tatactcata tattttcctc ctattcttct ccaaagaatt tcttttgagc
    16201 ttctatattt tcacttttta ctttagagtt agctaacaac tcacctatac tacttatttc
    16261 gctatcatta tcaagtaatg atgttgttcc accttctagt cctccagttc ctccaatttc
    16321 atcacctcct ttgttttttt cattattgaa taaataagaa tcgctttgtt gataagcttt
    16381 tatttgctca tctaagccaa taattttacc atctacaaag ttaatattct ctttatttat
    16441 taaagctgcc aaagcttttg ggtttctagg attgtaactt tcaatagctc tatcaaaagc
    16501 tgtattaaat tttaaaacct ctatttcttt ttctgcattt tctctaattt ctttatttgc
    16561 atttttaaga ctttcaattt catctgataa ctctttgtta tctttaactt tgccttgtaa
    16621 atcatttaat tgcttatctc tatctcctat ttgcttttta tactctttaa tctctttatt
    16681 agcattttct agctctgtct tttcaacgta tctaggactt ttaatattat ctaaaagaag
    16741 cttattttct ttatctttag ataatttttc ataaactttt tgtccttctt catctccaag
    16801 caacttttta aaatattcta acattcaatt tcctccttaa aattaagcat aataaaagca
    16861 ctcattaacc tttaattaat aagtgcttac tttacttttg tatcaattct tttaactttt
    16921 ctttgtactc agcataacta ttgtattcat catagttaaa accaggtgca tttttaccat
    16981 acttttcttt atataatcgt ctcaactcta acagtttttt atcttttctc atttcttcaa
    17041 gcaatctaaa tccctcctag caattcatta aatattttat ctaaactatt taaatgttct
    17101 ttgataaaac catttatttc tttattattt tgatacttta gtgtaaataa attagcaaat
    17161 atttcttttt ctttatttct gtttttactc caatactttt cgctatgagt tgctaataaa
    17221 tcctcaaatt cattattaga taatgctcct aatatatcac taataaattc attgttgtac
    17281 aactcattag aattagtata tatactttgc aggttctcaa tattctccat aacatatata
    17341 gagctacttt caatagcttt ttggaactta atattattat agctcttaat ttcttttata
    17401 tcaattctat gtgcaaactc atgaagtaaa gcagcttctt tattataaga tttaaattcc
    17461 ctgatgtttg gattaatacc aactaaatca accttagtgt aataaacaaa aggtattttt
    17521 tgactattat ctattattat tctgtttgga tttacatact tattaatata tttctgaacc
    17581 tgtttaggtg ctttcttcga cttattcttg attgaattag taatgttctt tttaattcta
    17641 tcatctttac tatttgcagt tattttattc tttttatttc tttgtttctt aatgactgga
    17701 acattattat tttgatatat tactccatca aaccaactat caagaatatc attatctcca
    17761 ccagttaccc aatcattcat cattttagaa gcttcattga ttgttataat tacttgaact
    17821 ggataacata aacaattagg gtgtggaata ggatactttt cgggtgggaa aactccctca
    17881 cctagcccaa aacgattttg ttctgcatac tcgtcacatt catcctcgcc cctccatttt
    17941 acttgtctaa tataatgttg tgagcttaaa ttccattgta atcctacgca gaaaggatta
    18001 tttattgcat tttgaacact tgtttctaca aaagcatgtg ttatagaggt tctagcaagt
    18061 ctctgagctt gataagatat acttttattc attccaactt ctagtgtttt agcttctgtt
    18121 tttctaactg gattaatata attatctaag ttctttgcta aagtctttgc attagcgcct
    18181 cttgcaacat tagcttttat taatctatca atgtcttttc tatttttatt actatagccc
    18241 caaattttac tatctaatga ccttttatct ttataaaagt tacctgtaac tagtttttcc
    18301 actacgctag tagttgtctt tatacacata gcatcacatg caagatttat agatttattt
    18361 ggaactattg attgatagta atacatttgt aattctttag atatattaga agcttctatt
    18421 acgctttttt ctgtaatagg tactaatctt tgatttaact cgttaatgta cttctcgatt
    18481 gatttattta gcttttttaa gtatttagta cttagattta actctttatt tttagcaata
    18541 tcacttaaaa tagttttact agcatccttg tagacattta atatatctaa ttgtactttt
    18601 ttatctaata ataaaagttt ttttctagct tcaagaactt tctttgtata agcattgtcc
    18661 ttcataacaa cacctcatta ctcatcacta ttattagatt tactatttaa ttcttcgtca
    18721 atatcatcaa cttctatatc tgcatctttt ctgaattggt cttgttctac tgattgtatc
    18781 ttttcattat cttctagcac ttcattaaat gcttcctcat aatcttcatc atctccaaat
    18841 tcttttatat aatttctatg acttctaaca ttattattaa cttcttcaag tgccaatctt
    18901 tttgaatctt cttcatcttc tggaattgga taatttttat ttaatacaat agaaaacatt
    18961 aaatcatccc aatcatgatt ccaatcatca taacaattaa atttactaca agcttcaact
    19021 attaatctta acatgcttct tattgcaggt tcccaatcat tccacttttc tgaacatctt
    19081 gcaataagct ctgtatataa atatttcaaa gctttagcac taggtatatt ctgtaattgt
    19141 tcaggtctag gtatcgctaa tttttcatac atactatctt caagtctttt aaaaaaagag
    19201 tttacagggt ctgcatttga aaaactactc tcaaccctat acgcttgtgc ttgttttcct
    19261 ttttctgaac cttcttcaag tgtttttaat gccattaaag cattcggagc aatcttacaa
    19321 gcattaactg tttcttctgt cgcatctact acaacagttt gcccaaacat cagaaatttt
    19381 aaagagtcat tgaaatctga taatctttta ttataggtat cttgtaatgg ctttaaatcc
    19441 tcaatatcgc taatgcctgt gatatttgta atactttgtt cattaacaat tacccaacat
    19501 ggtattttag aaagcttggt atcactttct tttacttcaa taggtttaga taagttatca
    19561 cctttgaact tttctatctt tataaagcag ctttcttgat tactgacatt actttttttc
    19621 atatagtaag tatatctata ccatatttgt tttgccgtta cttcttttat agttgatgaa
    19681 tcaaatctaa caaatacaac tgactttaat tttgtaatat cattactatc aacttgatat
    19741 ttgaaatcat ttatagaatg ataaaagagt cttataggtt gatttggttt agcttctaac
    19801 cttaataaga ctctttttgt tatagttgct atcttaaaag cttttagtgt gttgctccaa
    19861 aacttgctgg catttaatat tgaatcaata tactgcctta attcctcaca tgcttcttta
    19921 tgttcttttt catatgcttt aagtattata gttggctctt ttccaaacat aaaacgagct
    19981 tgtttattta taagtggttt taccttatta tccacaattt gagaaggtgt atagtctagg
    20041 ttatcaaaag taatccaact ttgacctaaa aaatcatcat ctaacagtcc catagtttta
    20101 tttctacatt cacctagata aaacaaaaaa tctctttctg catgttttct atctcttaac
    20161 tctttttcat ctaaattgag taagatattt tttatattca ctaaaataca gtccctcctt
    20221 tcttattata ttgattttgt atattatttt tcttcaatcc taagccttta ttatagattt
    20281 catcatcata attaggttct tttcctccaa ataatattgt atgaacaaaa tatctaattg
    20341 catccatggc atggtccatt actttaactg gtttatcttc tccatattct aaagcttttt
    20401 catcccaaac ataagaaaag aactccttaa atatattaga gcaacaatca ttaaatttaa
    20461 tcatttctct atttaaagca gttccgacat ttcttattcc atttagtaca tcattattac
    20521 cctttttaac tctatacttt cctttatctc ttatcaaagt tataaaagaa gctgcacttg
    20581 ggtctattat tatagcttta ggatgtatat tccctaaaaa atttactagc tcagtataat
    20641 atttattatc tgaattttgt tttccttctt ttcttccgtc gtaatagtat tctttgactg
    20701 cataccaaac atctttacat ttgccccata atataaatac tgtagcgttt tgagtaccat
    20761 aatcacatga tacataatgc tctgtgtatt ctctaagttt agttagtacc ttatgtatct
    20821 tctcattaaa catatcatag ataacacctt cggcttgaca ccaaagacct aaaatatatc
    20881 tcttataaaa gacacctgta aacattcttt taaacttatt tttaactttc tcagataaag
    20941 ataaattatc atccattgtg aaatgtaaat aacagattaa tttttctttt actttgtcta
    21001 taaattcagt tttaataaaa tgatatggac ctgcagggtt gcagttcata aatattttag
    21061 agccatcaac agagcaacga ccaatcattt ggtcaacaaa gtttttagga aataaagcta
    21121 cttcatcagc taaagcccct gctgctgtca aaccttgtaa tttatcttgg gaagcttcat
    21181 tgttagcatc atacatataa tatgtattac taccaattac taaaaaattt tctgaacgat
    21241 tgtactcaaa tcgccaaccc caagcattta gtatctgttt cataggttca ataacatttt
    21301 tctttagtga accaatagtc tttcctgcaa ttatgaagtt ttctacttca aaattagttt
    21361 gtgtaaacat taagaagcta caaatcatag ctatagtttt accacttctt attgctccat
    21421 cagcaatgat aatgtcatag tccttatatt tagaaccttc tctccaccag ttgagaagtt
    21481 ttaattgttt ctttgaaaaa ggtttaaatt caaatctctt ttcttgtctt ttttgttttc
    21541 ttctaagtag agccaatttc attttctcct tcttcttcat taaataaaac ttttatatct
    21601 tcttcactca tggtagtggc ttctaagaag ctttgtatag cttctgaatt atcgtattta
    21661 tcatcaccaa acaacttaat tttctcaagc tctactttag ttctcattgt atctattctt
    21721 gtcttctgct cctctgtagc caaattccaa tccttatgaa tcatttcatc atactgttta
    21781 attaaacttc taagttcact cattgcccta ctctgtgcat taagaaaaga tgcttgccta
    21841 tcccatgcaa attgaaattc atactctatc ttctcaccat tttctgtgct ttcatgtttc
    21901 tttaattcct taatcatttc ttctttatct ttaacataca ttatcttttg tgctcttatt
    21961 attgctgcat attgaattgt tatctgttcc caaagaatat caaatttatc ttttatagat
    22021 atttcttgta ttaattccct agtttcttca ggtaagtatt ttgagaagaa accaaatttt
    22081 tcagcgttct tatttccagg aggaccagtg gcatttttat tacctatggg tgcacctctc
    22141 tttttggttg caactttttt tggttggttg caaccttttt gtttccagta tctagttgcc
    22201 catgatttta cagtcgataa acttacatta tgtttttcag ctatttcctt gtacttaagc
    22261 ccttttaagt aatcttcatg ggctaaatct gccttttcat tcataccacc acctcgtttg
    22321 tttgtcgttt tgtgaataaa aaaagaactc tatttgtcga gtcctttgat tatatatata
    22381 ttatgttttt tattttactt atgaccatta gaaagtagcc tcattgtttt ttccaacttt
    22441 ttattttgtc tcattgcatt tataggagta ccaatttgag catctttaat ctgtttaatt
    22501 gcactctctg gaagttttgt ttgaatgtct ttaagttgct taaccacgcc tcctatttgc
    22561 atatctttaa gtgagctttg agttgtcatc ggaactccaa ttcttcttag gctactgtgc
    22621 gtttccttta ctgtttcata taggatactt tcaaagagtt ttgactcttt ttctaattca
    22681 tttatatctg ttacatcaca gatttcagat atctctttta taaaacctgc aactgtaaag
    22741 gaataaaata atttgccagt ttcacaaaaa aattctctta taaggtcttt tcttgtacct
    22801 acatttttcc ccttaaaatt atcacaccaa tcttcttttc tatcatcaga gacaaataaa
    22861 atatctgttc ccttttcttt tgatatattt atcatttctt tccatataac taaatctcca
    22921 taacaacctg gttcaccttt atcgtcttca tctttatacc ctggtggagt ttttgattta
    22981 tatctttttt gaccttcttt atatatttct accaattcat tttcagaaaa tttttgatta
    23041 actttaccat caaataaatc tagaatttta tctaatatag ggtctgtttc tcctaaatct
    23101 gtaattaaat tagattgatt gtacgaatct atagtatttt ctatactact aataaaacaa
    23161 tctatatctt ttaacatttg tgattttaaa tcactttcat atttgagtaa acagcaactg
    23221 tctttaaact ttcccttaaa atttacgttt tctatagtat ttcttaattt ttttatgtct
    23281 tcacatacag tttttttcat tccttcttta aaatgagctt tgttatataa aatctctgac
    23341 ctatttttgt aaaactcttt aactgaatga ttagttaaat atatacgttc acttaccttc
    23401 gataatattt caaaaaattt atttttattt tcttcattat atctataaag attcaaaagt
    23461 gcgtttgtat caagtataat taacccatta cttaaaattt gttctatctt tttatttcct
    23521 ctcatattat attcaaaaaa aatatttctc attagcccct cctaagtaaa atatactata
    23581 tttaaattaa tacaataatt gaccattgta attaaaaaaa tttttcagaa taacaaataa
    23641 aacatacaat ttgctttatt aacatgacac tcttctgttg cccagtattc atatttagcc
    23701 ataccatcat cccatcatag atagtcctca agtttcaagt ccaaacactc tttttcataa
    23761 ggatatattt ctttaaatat attacaaata taataataaa aattatcatc actatttaaa
    23821 ccactaaaaa atactgtcaa tacattccaa gctattttca ttgtttcatc atcgaaaaaa
    23881 agtacagttt tgacaacaga gtataaatca tcatatccta atgagccatt attatgtcta
    23941 atttgttttg ctccacaata aaacactgat agtatacata atttcattga attgtcatgt
    24001 ctaataaatt tgaaatattc tctaaagaac tctatgtggt tagattcatt ctctttgtta
    24061 gaaatatctt tatttaaaga agatggcata ttttctttat ctacttttaa caccatatta
    24121 gataatccaa ctgaaatctc ttcttttatt tcatctttca tatcacttac attttctaat
    24181 ttataagttg catcttcaac gtttccaact atgtcttcta attttttaga tatatttaaa
    24241 agactatctt tagtattatc tgatttggtt tctcctatta aggttaatat aattgctaaa
    24301 acagataata ttattcctga tagagtcgcc ccaaaagcaa tccattgtga taagtcttta
    24361 ttatcatatc ctctatctgt tataacaacc actaatgcta atattaatat aactataata
    24421 taaaacatgt gaactttgag tttatctttc gaaaaaattt tatctgaatc cataattcct
    24481 catctccctc caaagtataa aattcaactt caaaggtcaa tatccttcaa caatcgttcg
    24541 acaactacaa aataattcta aataatgctt ttatcttatt acttcccaat cctctgcaaa
    24601 tatatctcca gtactaggaa cccacataga atgcgaatta tcagcatttt ttatctgtaa
    24661 atatgggtta catttaaaca aatccccttc tttcaaattc catacctctg ctgtttgctt
    24721 attacaaggt attccagcag gataaccctt ttgtctaact acaaacattc cttttccatt
    24781 ccagcctact ctaaatgctt tttctccatt ttttattaat ggtaatactt cttcaaattt
    24841 cataatttta ttctcctttt aatttattgt ataaaaaaag actctccatc aagatagtca
    24901 tttaaatcat ttctattaat tccttaatct ttttatatac ctctttataa ttcatatctt
    24961 tatctattag cttaggtaat ttcatagata taattctttc gagtgcttgt atatcaaaca
    25021 gttcactttg atttaactca tctcttttca caccttttgg tatacctaat ttttttctta
    25081 caagttcagt aaaatgttta taatacatct gaggtttatt acttccttga ctagtagcat
    25141 aataaacaaa ctcttgtatt tcatctgtaa aatcttttct tactttttta ccttctgctc
    25201 ttatatccag ccattcttgg tctttttata ttccctatct atatttctta atgtgtcatg
    25261 aatatttact atgcctaatt cctctcctac atctccagca tgaaaccaaa cttcttctcc
    25321 gtttttggac catatcattc ttacattttt ttcttgtaaa attttcaaca tactactacc
    25381 tcctaaatta gttattaata aagaatattc agtttgaaca ctcttatttt caccccccct
    25441 caatacgagg gacattaaaa aatcgtatta aaaaagacct agaaattaat ctaagccttt
    25501 ttaatggggg atacatattc tttaagggac caagccccac ccgattttta gacttctgaa
    25561 ttaagataca aaattgtatg agattttaat cttaattcaa ctactacata tagtgtatta
    25621 ataggttttg aacatagtta gaattgaact aacagcgtcc tcacgccctg cctagtctgt
    25681 tcgtagtgac tagggcaatc ccttaaccct agtcaaatat taagttttga gagggaaatc
    25741 tttatttcca caatactatt atctcatgtc taaaacaaaa aaacctgcac attttcagca
    25801 ctcaaaatta ctcttacttt ttaatttagt ttgtaatcta acaattcaaa aagtggttct
    25861 tgctctatta atgctttctt cccaaacagt gctattgata ttgagctaat agcttgacta
    25921 gctcttacac ttaattgtct ttcttctaaa tgtactatat caaccatttc ttgccacatt
    25981 agaccatcta tatatttaag ttcaataatt tgtctgtgta taggttttaa atttcttatg
    26041 gctaaatcta ttgtagattt aattatttct gcttcatata actctatttc tttttctgtt
    26101 attaagtctg atacattaac aatagcatcc tcaatcatat tgctagtttt gtttgtttta
    26161 cctgttttga cactatcata acttatccct ttcattaaat ccccaaccga attatctttc
    26221 aacatttgta tttcattttt tagttttatt atatttgtac ttaattgttt ataattagaa
    26281 agttgtttct tagttgcatt aaaaaactct tttttagttt tagacatact cacacactcc
    26341 tatcaattat ttatgttata ataatatttg catataaaag ttttatattt ttgacaagta
    26401 ggagcgtgaa gtaatgctcc tttttttctt tttacttact accaaatcct attcttacag
    26461 aagattctat tactgcattt aaagtattat tagataaaca ttcactttta aactctctaa
    26521 tataatgatt ttctttgtat atagctgata ttttaaataa atttcttata tatactctat
    26581 gcctatttat ataaataaat tttttcatat tattaacctt aatattctcc ttaactaaca
    26641 tcttccagtt caacctcaac tcttggtctg tcactataat atttcttact cactacttct
    26701 actatttgag aatcatcttt ataagctata ccatttaaac tgtcagctac agacttaatt
    26761 atattgtcta aatcgggttt cttatttggt cttattaacc cttctacctt gtctatagca
    26821 ttcttatacg ctttagtatt taccttattt ttctgtagtg cttctctatc ttttttagta
    26881 atgtcaaagt aacaaataac tgtcattttc acattaccct caaagaaatg ctttactgta
    26941 gagttataca tgagtctaat ccagttttca tataatatag tttggtcagg tgtataagcc
    27001 ttaccatttg ctgtactcat tctaggacgt gctttcgcct ttggttctcc atctattgta
    27061 aaattaactt tcattggcta cctcatttag ttctatttct tctttatctg taagcttaaa
    27121 ccaatatggt ttatatccaa attcatctat ccatttttca aatacttcgt ttaccctatt
    27181 actaagtatt atcatatctt caacacatat attttcaaac caatcttccc cacactctcc
    27241 aacttcatca tcaatctttt tttgaataca ttctaatgct tcttctatat ctatgttagg
    27301 tatactaact tcctcttttt taccaacata aatactttct ccaaaatttc caaactcttt
    27361 aagttcttct ttagctgctt ggatagcttc ttctttactt tcatactcat cactacttaa
    27421 atatttgtta tcccagctat ataaccaaac atctttttgc atattaatac ctccactatt
    27481 tatttttctt attagccttc tttctacagt ccttacaaca ataaatctcc ttagattttt
    27541 ccttaagata aaacagcttg ccacaccaac tgcatcttct tcgtttcata aactcacttc
    27601 ctagtcacaa aacatttatt ttattcctaa aacaacatac ccatcttgca gataattgct
    27661 attatcaagt aaataaataa tttctttgcg tatctcaaga cctgtgtatt tttctccatc
    27721 aaattcctgc aacaccaaca agtcgccttt tttaaaacct ctatcattct tcctaacttc
    27781 aaaatttttg ttcccattta caacttcttt aaaatattga ggtaatattt ttaattcatg
    27841 tatcataagc tcacaccctt taaaagtttt agtctatttc acctttttca acttgttcat
    27901 attctatttg atatataact tttttatatg ctatttgacc ttgccattct tttattactg
    27961 cattttcaat atcttgtata aatatagcta acttgccatt tacatttgct atttttaacc
    28021 atataaattc atgtgtatct ttattctttt ctacccatat ggtcatttcc ttattttctt
    28081 ctaaacataa atcatttaga aatatctcat tatttatctt atacatttga gtagtaatca
    28141 taatctcacc tccttcctat aagtcaaagt aagtctataa cattctagtt tcattcacaa
    28201 acttaccttg actttatttt tataattatc tttcagcatc cttctctagc caattctcat
    28261 atgctatatc acaatcttta ctttcgcaat ctaccctatc gcttatgcaa ctagcacaaa
    28321 tctctttccc aaattcctta tatatttctc tttcatcaag attctttgac ttgcacattt
    28381 ctttattagt catatgctca cctacttttc ttcgtaaaat tttacattct taataattat
    28441 atctatagac ccattttgat tttgtcttac tgtatatttc attgggtcct caaaatcagt
    28501 cagattacct tttatatcaa agccattgtc agtttttata tttctctttt taagcttttt
    28561 ctcaacccat tttttatcta tactaaatcc tttatcaaga cctttttctt ccatatgttc
    28621 tttaaagcta tcttttaact tatcatcttt aattgtttta tcaacaaaat tatttatatc
    28681 aatttcatgc ttttctttca aagtataatt taatatactt cttacatctt ctgcctgttt
    28741 tatatcattt ccaagagcat tagttatcca attttcggct gtacttttaa acatcttagt
    28801 cttgtacttg tcatctttca ctttagtagc atttagaaac tctgtaataa acttagaatt
    28861 agcttcttcc ttttctgcat ccttgtctaa aaccctaaga tgatattcgt cattcattcc
    28921 actcaaacca accaaagcag caatttttac tgtcttagtc tcttgtatat taatttcatt
    28981 tttagacatt tgtatattaa atttatcatc tttaaactcg attgaatgag tatatgactt
    29041 gttgtaatca agctttaata tagcaacttt cttttcatct ttttgagagt ataaacaaat
    29101 tgctaagtcg caagattcta atgtagcatt caatttcata acatcaaata aataagctgc
    29161 aatctcttta gagttattta aaaatgaact ttcatcataa ataatttgtt cgcaacactt
    29221 cttaattaaa ttgttactat agtcattaaa tactgctgtt ctaatgtctt tagctcttga
    29281 tactgtctta atcttcttct gaaaaaatag atccatatct tgactaaccc taccctcaaa
    29341 gtcatttagt attggtgtat cgctattctt atctaaaaca tgtattataa atttgtgtat
    29401 tatcatattt caattcctcc atcacaataa tattcagtca ttttttgact tctagtatcc
    29461 tttataactt cttcaacttt atctattgtt acaagcagta tcttgtcatc tttagctaat
    29521 aattctgctt tttgttttaa catttcctta cttccacaag aataatttat cttacgttca
    29581 tccaacaata accctttttg ccatctaagc acatactttg acatttattc accccttatt
    29641 ttcatttttg agagttacaa aacacttcaa caatactcat actaaaagac attttgcaac
    29701 tcttagccca ttctttttgc tatttcatat acaacatttg cagtaacagc atttcccgct
    29761 tgtttgtaca gttgactatc tgagcataca cttgctgctc tttcgtaata tttatccgga
    29821 aatccttgca acctaaagca ttcctttggt gttaaccttc ttatatctcc atttttcaaa
    29881 attccatgtt tatcttgagc tgttagtgtg aacattggtt ctccgctttc tttaattcta
    29941 cgaccatttt gtcttttatt taccctatcg ggcgttaaaa ctgcattaac taaaactcca
    30001 ctattatcac aatttctatt tgtcacacct gcattatatt ttgctttaag gcatctagca
    30061 tttattgtta ctttagagtt tttatttaag tctataaagt atagacctgt tttagcacct
    30121 ccacctcctg cctgacttct aatacatcta gcaattccaa ctgcatcata aattctattt
    30181 gtactatgag ttggattatt tagttgctca agattttttc cactttttct ttcgatagga
    30241 aatacttttc gtgtacttcg tcctctaaaa tgtccaacaa tgaatattcg ttctctattt
    30301 tggggtactc cgaagttttt agaattaaga acttgccact ctgcatcata gccgatttca
    30361 tccagttcaa cgagaacttt gaggaaatca aatcctccat taacactaag tagattttta
    30421 acgttttcaa taagtaaata cttgggtcta tcttcttctt tgagttctct aataagtttt
    30481 gtaactgtaa aaaataaact tgaacgttct cctctgaatc caaattgttt ccctgcaaca
    30541 gaaatgtctt gacatgggaa tccaaaacac cagacatctg ctcttgggat attttctgtt
    30601 ctaatttctc taatatctct ttcaaaccat tcatcctcct tcggtttgtg catggcatta
    30661 taacttaaat ttgcgaattt atcatattcg caatgtccca aacatttatg tcctgctttt
    30721 tccatcccta gcctaaagcc acctatccct gcgaataaat ctaaaaatgt aagcaataca
    30781 acgcctcctt attttcattt ttgagagtca caaaacactt caacaataat ttatactaaa
    30841 agatattttg taactctcta aactgtctta attagatatt ttcacttata tttcttctaa
    30901 catttcctcg agtttatttt ttaataaatc atatttttct ttagtttcta aatctaatat
    30961 tctaactctt cctcgctctg ctataatagc tatatttgaa ctttcacata tcatctgtat
    31021 ataatttaca gaagcattta tcatttctaa tctatcatcc attcttatac acctctttta
    31081 ttgtcgcaat tttcacactc ttttagattc aatctatact cataaactct accagttata
    31141 aaacttccta ttactaatat cgcactagct aagatattca ttttcaagac tctccttacg
    31201 ttctaagaac tctgttctga cttcctctaa attctcatat tcattaccaa ttactttgta
    31261 atctctacta tgatatattt ctgttttatt tataagctct atccattctc cttcaaactt
    31321 ctttaaataa tgccattcca aatataactt aatactatta aaatctcttt ttacaactcc
    31381 atattggctt gtatcactag aattatcata tcctttcaat atatcccctt cacaaatttc
    31441 ttgattgttt ctagcccatt ctccagagca taccccaaca ttagagactt tctgccattt
    31501 gcaaccctct gtcaacataa ctaaacattc aagtatatta ctccacatta ttgttgctga
    31561 ataaatccac ttttcgttct caaaatcata acctctgtac ttaattaaac tcacttttaa
    31621 tcatctcctc atattcttct ctagccttat ctatagcaat aaatatgtcc tctccattgt
    31681 catacaactc ttttgctctt ttaattgtgt attcagtcct tgaaacttcc attattcctc
    31741 cttaatatat tcagctttcc agccacttct tgttttagtt ttctttttaa ttgtttggta
    31801 aactgcctga ctctgtagtc ttaaaaaaca cgctgcacta tctatagaat caaatatttt
    31861 ttcttcacca gttttggcat taatcaactt tacctttgaa cctttctttt tcttttttct
    31921 atttttatca acattaaact ctattagcat tttttcagaa gttggaaata caagctctcc
    31981 attttttctt actccataaa cacaacaata tagtgctaag tagtttctaa gagtcaaatc
    32041 atcttcaaat atgttatctg caacagaacc attaaaatat ttttcaacct ttaacatttc
    32101 agctccctcc ttattcaact ggcatttcaa acactttttc tttgttgcat ctaaccccgt
    32161 ctttatttat aatgtcaaac ttagttcctg caataatagc ctcctgtatt ctgtttaata
    32221 cttcaatagc tctttcatta ctttcatata cacctattcg tttgacatca tcttcaaata
    32281 ttacaaatac ttgtttatcg tatatttcaa ctctgttaac tctcattaaa tctaatctat
    32341 cttgacttct aattattatc attcctaata cccccatcat catttttctt gttataattt
    32401 ttaagacttt ccgcatttca agccacaata tatctctcaa taaagaatcc catccagata
    32461 ttattgtaaa agtcccttca tattttactc tctcaaacgg attgtctatt tctgtaccct
    32521 cgaatgttac ttcttcaatt cttgtatcat ttatctcaaa acttccctta tcgcattcta
    32581 agaacacttt ctcacactca tatttcactc ttagacctcc aatatttttc aactcctagg
    32641 aagtaatatt gtataatcac tccctagact atttaactta attaaaaagg tatatcgtca
    32701 tcatctattg cttgaaaacc ttgtgggtct aatcctggtg gtacatattc ttgtttagca
    32761 ttattatcat ttttactaga aagtagttct aaagcattta cattaacctt agtaatagat
    32821 ttccagcaac cattttcatc tttgtaatta tatatattta actctccaac agcataaaca
    32881 ggcttagctt taacaagata ttgcactaga ttctctacat gttttcctaa ttgctcacat
    32941 tgaataaaat cagttatttt atttccattt ttatctttaa accttctatc tactgccatt
    33001 gaaaaggtta tttttggagt acctgaattt ggaaggtact tcaattctgc atctgcaact
    33061 aatcttccaa ctaaagttat tgtattcatt taactagccc ccttctattt ttcttcctgt
    33121 tcttctgtat actcaacaaa gtaagtataa gttgtcttgc tattttgctt ctctctagca
    33181 acctttactg tatatccagc tttcccaagt aatcttaata actccaatct atcttgttca
    33241 tttaaagaac cacttctttg tgcatatatt ctcgccattt tatacctccc cttttctagg
    33301 aagcaatata ttgatattta cttcctagaa gtttaatttt atttaaattt ttccttctga
    33361 ctctttttaa taatctcatc tagctctttt tcttcatatt gagtgaaagt ctgattgaag
    33421 ttagcaaact tatttttatt cacattatga gtattcacag ctttattatt agactgtttc
    33481 ttctcttgtt tactcttttt cttcctctca aactcatttt gatactctgt aagttctaaa
    33541 acagttttta cacctgcttc tatccaatta tttaagattg tttttacata cttataattc
    33601 ttaactccac tgcctacagc ttcatcaata gctcttatta ttacatcagc ttccattcca
    33661 tcatctaaat aagtcagtag ttgaagaaaa ttatttggag taatcacacc tatataagat
    33721 tcataatatt tttttatgta gacagtctta tttttttcag attgttcagc aataacagta
    33781 gtaataacat cattttcttt taaacctatt ttctttttaa tactattttc ttttatgttg
    33841 ccgatttccc gacctcggtt ttgccggctt ccggtttcac cgacttcggt tttaccagct
    33901 tccggtttta ccggagtcgg gaaaacggca cacggttgag attcagtcgt ttcaacactt
    33961 tcagaattta cattttgagg tgtatcaaaa atatcatatc tataaccttt catttgacct
    34021 ttttcatccc ttatttgtgt cctaataaca aaaccttcct gcataagctc ctttaaagca
    34081 ttacttactt ttgtcttact atcttttcta tagcttatta atgattttgc atacacttta
    34141 tggctacccg acctttgaaa tcttaacatt tgagtgacta ctcctacagc tgaataagaa
    34201 agattttcat tgtcgaggat tgtattaggt actcttgtaa atgggtcgtc aaaatttatg
    34261 tgaaagtatg tttcattatt aaaattcaat atatcaccta ctcttgattt tgcttttcat
    34321 aagcattaca aattgtgtca tattcttgtt ttgttaaatc ttttatctct tttccaaatc
    34381 tcttaaatac tttctctttt aagctctcct tattaacatt tgcattactt gctattgcat
    34441 ataacctgct taattgttta tctgttaaaa ttctattgtt agaattactt ttagcttcat
    34501 ttttgccact agttgcgtca aaagtgtcat tctcagttat gttgagtaac tgaatgtaca
    34561 aatatctagt ttgataggtt tctatacctc ctagtgcctg taattcatta gaacctttaa
    34621 gttgtaaatc tctcatggga gaagtaaata caatctgttc tgatgggttt tctccattaa
    34681 ttaatgttag agttgcatat tcatttgtaa aggtcactat agggcatagc ttagcttctt
    34741 caagtaatcc agttgcttgt ggtagaaagt ctgctaactc aaaatacttg aagttagcga
    34801 acttattttc tccacttttc tttagattca acttactaaa tttaactctt acatccatca
    34861 atttaatgta aatattatta atttccatgg tcctcaccta ctctttttta gcttttggaa
    34921 ttgttagtgt agttccatat tcaatcctgc aaccttcaac ctcatgacct tttttaataa
    34981 aatctttaat agtgttctta tctactttta caacttgctc tactgtttta tatatagcag
    35041 gtatcttttc ttcatcttct atgactaagc tacctgctga ctttcttata cttatatttc
    35101 ctaaaactgt ttctactttt ttagtcccaa gtaattccat acagtctttt atattgcttt
    35161 ttaatctatc aagagtattc ttcttaacct tctttaactc ttgtaacctc ttaatctctg
    35221 aatctataga gtttatatca ctgtcaatgt ttaatattac tgacactatt ctagtgtttt
    35281 tattttgtat ctcttgtttt attatttctt ttatttcctc tagtttttca gcttcatttc
    35341 ctgttgtttc tgttaaacct tcttctattt ctaataaatc tgtagttaat tcatataaag
    35401 tactcataat ttccctccgt ttgtgctata attagcttat attttatatt attttggaat
    35461 cgagccactc ctaatggctc ttttctatat ctgaatatct ataggtctat ctctttcaat
    35521 ttcttctgaa attaattcaa atatcttgta atcctcgctt tcttcatatt cttttatttc
    35581 aatttgtgta tctataattt ttagtaatga ctcagcaaat atttttaatc tttcatttac
    35641 acttttttct cttaaagcat tacttaactc tatttcatct aatacatctc ttttttcttt
    35701 ttttctaagt tttgtataaa gttcctcatt tttatttatt tctaaattag ctctatttag
    35761 ttgttgctct actgcatttc ttactatagt taaacttttc atgattaatc ccccttaatt
    35821 tttaatttac ttggtaaata caagttaact aactctatat ctctgttgta ttttctaaga
    35881 ccttcaaaac ttgcttttat ttgcttatca ttgcaaaact gtacataagc tattagtact
    35941 cttacattca actaaatcac cccctctcta atttctttca tttctctaag catttctttg
    36001 atgttttttc cttgatttct agttataaaa tcatctaatt cataactaga aactttagtt
    36061 gcccctatat caactgactt caaaagccca ttttttatta actcatatcc aaatacttta
    36121 tctattttca atcttttact tgcttcttca acagacataa gataatcggg ataaccttta
    36181 cttataacaa ttgttagttc ttttggttct aataattcta ttttcgaagt ttcatttaag
    36241 tactttgaga ttttattttt atagttgttt aaattcattt ctacaacttt acggatacct
    36301 tctgaaaaac aaattgatat attatctagg tcattaaaac ttttatcttc ttgtttatct
    36361 aaattgaaat tagatatatt gcccaattta ctcactcctt ttcaaaatat tctgtattta
    36421 atttttatct tccaactagt tcatctaatg taatgtctaa atagtcggct atttttatta
    36481 atgtatctat agttggattt ttattttctc ctcttaaaat tgcatataaa ttccctgaat
    36541 ctacacctat ttcttttgct aatttccatg cttttaaatc tctatctttt aaaattttat
    36601 ttatgttgtc attaattgcc attattttcc tcctttgata tactatattt gtaggatata
    36661 tcctatatct ttttatgaaa gttggtgata ttatgcagtt cagtaaagat atattacata
    36721 ctcttacttt agaaattctt aaggaaaaat atgattttaa aagttcttct gaagaggaac
    36781 ttttaaaaca ttaccatgaa atctttttga aactttcaga agtcaataat agtttttcta
    36841 aaggcgatgg cctcagtgtc tttaaacaaa tgtaggtact aaattatatt taagagcttc
    36901 tttgcaaaaa tctaaaatat cttttgagga aaggatactt tgttcatttt cattcaatgt
    36961 attaagtatc cttttagcta tttgtaactc ttcttttgat aatattaatt ccgtttcatt
    37021 atttacattg ttcactacac tttcaaatga aattttcatt taattacaca ctccttttta
    37081 aaatattctg tatttagttt tcaaagtgct gttatatttt aacttaacat tgataattga
    37141 tttactaatt tcaatttgtt gataaagtat atttgacctt tgcctgttac ttttggtgtt
    37201 ttagtaatct ttgtacttcc gtcagggtta tatattgctc tttttttagt ttccattatt
    37261 tttaagtcaa cactcttttg agttggtgta ttgtaatctt cacccttacg ttttattaag
    37321 taaccattat ctctaagcca tgcaaataat cttttctcgc ctgtatcaat gccattttgc
    37381 ttaagtagtt ttgcaagttc tccaactaat atagaatcat ccgaggatgc tactgcatca
    37441 gcaaacaata cttttggttg ctgtaactga attaccttat ctttttcttc tatctctctg
    37501 cttttctttt ctattgtctt ttgagctact tgtaatgctc ttgccattat ttcgtcgtct
    37561 gtcatatctt ctgttgtatg tatatatcct ccagttttac gtattgttgg taaaacttca
    37621 tcaaataccc aactttcaaa tttttctgca tttggtagtt tactatttac aatcaatcta
    37681 tacatatcac tttcaggtat tgtatttact tctaatatct tattttcatt ttgcggatga
    37741 ggtatgtaac tttttgttac ccacctacaa tggtcattta ttgctttact tgtgtttgca
    37801 tatcctaaac atttagctat atctgttgca acaaaatatg gtttcttatc aacctcaacc
    37861 attcttattt gaccaaattc caatttttca aatatctgta gattattcat atttattcct
    37921 cgctttctac attgaattta ttttcttttt ttattttctc aataaactcc caacatgcat
    37981 ccacaataat agaattttta ctttttccac attctttggc tatattctgt acatattgat
    38041 ttaattttgg gacgagcctt actgtcattc ttactttttc cattcttatt cctccctcct
    38101 tatgacactt attcactgtc tataatcatt atattatgac actattttag tgtcaagact
    38161 tttttaaata ttttttgtat aatgtcatta gggaggtgtc aaaatgacta ctattactgt
    38221 aagaatatat acaccattaa atgaaaattt agaaaaaatt tcttatcaaa caggcattct
    38281 caaatcttcg cttattctat acgctattaa tgatattatt agaaattcaa aagttaatga
    38341 acttcaatca atctcgtata aaagtgatga tactgttcgt tctactctta ggattcctgg
    38401 tgtcctaaaa gagttgctag agaaaacagc taaagaaaat aatttatcag tcaattctct
    38461 aataaataat gctgtgcatt cattttgcat atctcattgg ttaatttatc tttgatatat
    38521 acaaccaaca cgcaattatt acaatagatg caataggcat tccgatttcc ttacttaaat
    38581 ctatcaacgc tttgtacaag tcttctggta tacgaagcgt tgtttgctcg tgtttcaatt
    38641 caatcacctc ttttgaatat tctgtattta gttttcaagg tactgttatg ttttaactta
    38701 acattgataa ttgtttgcta cttttaaatt tattaatgaa gtatatttgt cccttaccag
    38761 taatcttagg tgttttagta atacttgtat gaccatctgg atgtactctt gtaccttctt
    38821 ttgtttctat aactcctaaa tctacacttt tttgagttgg tgtattgtaa tcctcacctt
    38881 tacgttttat taagtaacca ttatttctta accagtcaaa taatctattt tgtcctgtat
    38941 caattccatt ttgtctaagc aattttgcta attctccaac taggattgaa ttgtcagaag
    39001 acgctaccga atcagcaaac agtacttttg gttgttgtaa ctggattacc ttatcctttt
    39061 cttgattttc taattgtagt tgttcttttt cttcaacttc gattaataac tgttgcagtg
    39121 cttctttata tgtagttggt aatttaggtt gttgttcttt taactcttgc tccatttctt
    39181 caaacttagt tacataaatt gctgtaaata taatcccttt ttctcctgtc attttattag
    39241 ctaccatatc gcaacctttt ttagttaata agtagcaagg ttggatttta ttttgagtat
    39301 ttatataagt actttctata aagaaatctt gactcctcaa atttgatgag tcctctaaaa
    39361 tcttcttgta tcctcttata tctcttaata aattatcgtg cttcttttct attaattctg
    39421 ctacttctct actttcaact aaaaattgat tattttgctt gattatggtt agattcttca
    39481 ttattattgc ccctttcttc ttttattgct attttagcaa cttcatctga aaaaaaataa
    39541 tccgcagata cattatataa tttagatatc ttttttattt cacttgcttt aaattcgttt
    39601 tttcctttta attttaatct aaaaccatat gagctaagac ctaatatatc tgccacattt
    39661 ttttgggtgt gtctattttc cttcatcaat ccttctaatc tatttaagta cattaaatca
    39721 cttccttttt gctattttgg caacttcata tttatataat atcaagtttt gtgattatag
    39781 tcaatacttt tgttgctttt ttagcaaaaa aaattgtaat aattaatttt gttgctataa
    39841 tataaataaa agttgctatt ttggaattaa tataaaaagg ggttgtgcaa attgaatagg
    39901 ataaaagaat tgagagaaga aaaaggcatc tcgctagaca aattaagcga ggatttacat
    39961 ataaacaaat ctacactatc aaggatagaa aatggtttaa gagaacctaa gaaaagtaca
    40021 atagaagaat atgcaaacta ttttgatgtg tctacagatt atttattagg aagaactgat
    40081 gttagaaata gcttatttat aaataaaaac gaaaaagatt atgatgctga aaattttaaa
    40141 acagaaaagg agcttattga gaatatgtat cttgacgaag atatgaaaga agtttttaat
    40201 atatttagcg agctaagccc agacgcaaga gaaaaagcat taaaagttgc agaattattt
    40261 ttactagacg aaaaaaataa aaaatagttt atttctgaaa atgaatataa taaaaaagag
    40321 gaatcattcc tcttttttat tgattttatg tatttctttc acttttattt tatattcatt
    40381 gattttattt ttatccaatt ctttcaactt tttcattaat aagtttaact ttaaaatatc
    40441 ataatatttc gtcttattca aatatatcat cccctataaa atattttatt tattaattcc
    40501 acgaaacata cgttcttaaa aatagttaca accacctctt tttcaaagct taaaactata
    40561 aaataactgt aaaatattat tatattttat agcttctatt atttttgctt tatctagata
    40621 aagttatttc ttaactacat tctagcacaa atttccaaca aaaagtgtgc gaatattgca
    40681 catttattac aagaaattac acaaactaac atatataaaa ttatctaaaa ggtaggttaa
    40741 atatatgtta aaagagttac gaaaaaagaa gaaattaaca caaatagagt tagcaaaaag
    40801 agttggttgc cacagaagtc aaatttctag gttggaaaat aatgaggata aagatttaac
    40861 tatctctatt cttattgaat tagaaatagc tttaggattg gaagaaaaat atttagtaaa
    40921 ttattttgct gatgaatata ttaaaaaaag aaaattacat aaataattcg aatgtttcta
    40981 tcaaatacta tttttaatat ataatattat ttgaggtgaa taaattgaac ttaagtttta
    41041 ataaaaagaa aaaatttgaa ttaagcaaag aagaattgga attaattgaa aattggttag
    41101 gtaataaata tgcaggagat atgacgatac ctgctattgt tgacttttct ttagaaactg
    41161 atattgagta tgaaaaaata gttgtttatt tggcagaaaa agtgctggaa tcacgtgata
    41221 aaaaacatta aatatgtatc taaataaaaa agactattaa ttataaacat aaaactttgt
    41281 ttcttatagt cttttttatt tattttattt aaattgatat atgttattat ataattacat
    41341 aaaaaagctt ttgagaggag aaattttatg aaaggcggcg taagaaaacg tggaaagaag
    41401 tggtattact actttgatgc aggtatagta gatggcaaga gaaaaaaggt agaaagagtt
    41461 ggtggaaaca ctaagaaaga agctgaaaaa tcgctccgtg atgcaataaa tgaatatgaa
    41521 aacgctggta tagtgtttga tgaaacaaat atgagtttat cagactatct taacttttgg
    41581 tacaaagagt atgtacttct taattgcaaa tacaatactc aggaaagtta tagaaattta
    41641 attgaaaatc atatagaacc tagacttggt aaatgtaagc taaaatctat aaatccagct
    41701 attattcaag aatttttaaa taataaatca aaagagacat acacacaaaa cggagaagaa
    41761 aaacactaca caaaaggagt tttaaaagcg atttatgttg tattaaatgc tgctttaaaa
    41821 tctgctgttt acccttacaa actcattaag gaaaatcctg ttcaatatgc cagtatacca
    41881 aaaaatgttt taaaggtaaa aaatgagtca gataacaaga ctataacact agatgagttc
    41941 aataaaatac tagaaatata tcctaaaaat acaaatatct atattcctct acttataggg
    42001 tttcatacag gcatgagaaa aggagaaata ttaggtcttt gttgggataa tgttgattta
    42061 gataataata taatcaaagt tagaaaaaat ttaataaaga gaaaagtttc agaatttgaa
    42121 ttagcatcac ctaagacaaa aacatcaata agagatatta aaataggtga tactttgtct
    42181 agaatattaa aagaggaaaa attgaatcaa aaaaaacaaa aaattaaaat tggaaaatgg
    42241 tataaagaaa ctgagtatga ttgggtttgt agaaaaaaag atggctcatt tgtaaatcat
    42301 aacaacattg atgcttgtgt gagaacaatt aacaaaaaat taaaccttga cttcaacttc
    42361 cattgtttaa gacatacaca tgctacatta ttattagaaa atggagctaa tataaaatat
    42421 atacaacaaa gattaggaca tagtcaatta tcaactacta tggatacata ttcacatgtt
    42481 acaagtaaaa tggaaagtga aacagtagat attttggagg gtattttaca ataatttgcc
    42541 accgaaaaaa aattatggtg gcaaacaggt ggcaaaacga taaaaaacat tctttttttt
    42601 gctcaaacac tgttattttg ctattttata taaaaaagtc gtatttctca cagttatacg
    42661 acttatatta aattctttac ataagtctcc ttcagatgga agtttatctc ctggtttata
    42721 tatgccagat gctatttgtt tttttatgtt gtcatatagt tgttgatata aaggaacaaa
    42781 ggaatttact tctaaatcca tttctttttt ttgtctcttt tcatccattt tgctcacctc
    42841 cctatgtaac atttttataa cattatataa caagttcttt attacatatt ataacaagat
    42901 gaaatcgttg tcaatttttt ttaaaataat ttttaatttg acagtatttt cttttatttt
    42961 ctctagtgtg acaaattgct atttttacat aaaaataaga ctgttgtaca acagattaaa
    43021 ttgtttatac aacagccttt attcacttat tacatttata tttttactta taaatttata
    43081 ttaaaaaatg ccttatatcc cttatacatt tcatatatgt ctactttaga gacaatctca
    43141 tgtggtgcat gcatatttaa aactccaaca ccacagtcta taacctctgc accttgattt
    43201 gccaatatat gggctatagt tccgcctcct ccttggtcta cttttccaag ttcagcagtt
    43261 tgccatacta catttccttt attaaaaatt ctctttactt ctgacataaa ttcagcatta
    43321 gcatcattac atccactctt tcctcttgaa cctgtatact tagttaaaac tactccattt
    43381 cccatatatg ccgaatttct cttttcatat gcttctccaa aatttgggtc atatccagca
    43441 gaaacatcag ctgataatac cttactattt gctaaagctc ttcttacttt aatatcacaa
    43501 taatctcctt ctaaagcaat cagttcagcc acagtatttt caaaaaactt tgagtgcata
    43561 cctgtattcc ctttagaacc aatttcttct ttatctacac ataatgcaac tgaagtaaat
    43621 tctggttgtt cagtttctag tatagctttt acagcagcat atgaacatac cctatcatca
    43681 tgcccatatc ccaacaccat agacctatca agccctaaat ctctagcctt accagctgga
    43741 actacttcaa tttcagcact taataaatct tcttcaacta tattgtattt ctcatttaaa
    43801 atctttaata tatttgctgt tataggttct ttttcttcat cttctaaagg catattccca
    43861 attaatatat ttaaagcttc tccactaatt ccttctgata gtttcttttg catttggtct
    43921 ccagccaaat gaatcaataa atcagttaca caaaatactg ggtcactatc ttcttcccca
    43981 atagatatgt ccacttttgt tccatcattt aatataacta taccatgtaa agcaagtgga
    44041 atagccgtcc attggtattt ttttattcca ccataataat gagtcttaaa aaaacctaga
    44101 ttactctctt gatacagtgg atttggtttt aaatctaatc ttggcgaatc tatatggctt
    44161 cctataatct tcataccttt ttctatataa tttttaccta ttacaaaaag tgctactgcc
    44221 ttctctttat ttataacata aattttatca ccacaactta tgcttccttt ttccatagct
    44281 tcatctattg aaatatagtt attttcttta gccaatttta taatttcttt tactgaaagt
    44341 ctttcagtct ttgacttact taaaaactcc atataattgc tagaatattg cataatttgt
    44401 tcaatagtat tttcttttct ttcaaaatcc caagcattct taaattcgta ctttaagttc
    44461 atattttcct cctactaagt tctaattttt attgtttttc atgtattgct catattcttc
    44521 tggtgaaact actttgacac tatctagtct tgatctaaag tgggctctaa agttttctaa
    44581 atattctttc cttaaaattt ctctttcttt gatttcatca gcagaaagga ttccctcttt
    44641 atttttcttt gctaattcat taatcctatc taatttcttc ttgtcaaaca ttttttcctc
    44701 ctaaaaaacc tctatttatt taataaaata atttattgct ttttatatct aacttgtttt
    44761 tatattcaca aaaaaactca tctatattat cacttattat agatattccc ttttctagtt
    44821 cttccatgct tggacgagct atatttatcc taaagaatct atcatccact agattgtcaa
    44881 aaaaataagt ccctggaagc attgacactc ctttgtttaa cataaaattt gtaaatgctt
    44941 gagatgtata tcctcttggt aattctaaaa aaaagtttat tcctccacaa gattttctaa
    45001 cttttaattt accactcaat ttgtcattaa tcaagttttt tgccaattta tacttatcag
    45061 tatatatctt ctctatatga ctcaaataat tcttccaatc aaaattattc atatagtaat
    45121 acattgattt ttgaattaaa cctggtgttg atatatctga tgaatattta gcccataaaa
    45181 ctttttttaa caactcattt ggcatttcta caattcctat cctaagacct ggcataagta
    45241 tttttgaaaa gctttttata tatactactc tatttttatc atcataactt ctaattgttc
    45301 tattgtcctt tgattcaaaa acaaaatcac ttataaaatc atcttctatt atataaaaat
    45361 catgttcttc tgccaattct atcaatttct tttttttata tgttgagtaa gatattccag
    45421 ttggattttg aaaattaggc ataatgtata taagtcttgg ttttattttt tccaacttta
    45481 attttaaaat acctatatca attccatcat ctaacatagg tatagatatt atttttgcac
    45541 ctctactttt aaacacttca attgctccat tgtaagaagg ttcttccata aaaacaacat
    45601 ctgaataact tataagtcct ttacaaacta tatctatacc ttgttgagca cctgatatta
    45661 tttgaatatt ttccttagta gtttttatat ttccatcact tagatactct accatttttt
    45721 ctcttaattc ttcagagcca agaccttcat catattcaaa tattgaacta ccatctttag
    45781 ataatgccat atttacagca tttttaaatg aatcgatagg aaacatatca ttagatggat
    45841 tcccattgtc aaaatgaatt aaatcttctc tttgcacaat aatcttattt ttcctgttat
    45901 ctataccgct tttataagaa tttaaacttg atacaaaagt accactccct ataattttat
    45961 atacatatcc ttcattttct aaaagctcgt ataccttaac tatagtagtg ttatttactt
    46021 gtaaaaaatt tgatagtttt cttattggag gcaatttttc atgttctact aactctttat
    46081 ttattatcaa ttcttttata tgattaaata tctgtatata tttagttgat tttttctcat
    46141 ttaaatctat tctgtatttt tccaaattta tcaccgctca ttgtatttat attttattga
    46201 tgcttttatg tatatttgat attatttagt atgtatatat aataccaaaa tttttataaa
    46261 aaggggaaat atttatgttt tacgactatc acatgcactc aagtttctct acagacggaa
    46321 aatctacaat ggaagaaatg gtaaaaaaat ctattgaact cggtttggag gaaatttgtt
    46381 tcacagacca tgttgactat gatgtatatg ctgatgattc tttttcaatt gtctacgaag
    46441 attattttaa gagtttagag actcttcaaa gcaaatataa agataaaata tcaataaaaa
    46501 aaggaattga atttggagtt caaactcaat taatggatac atacaaaaag gaagcacatc
    46561 aatatccatt ggactttata atatgttcta tccatgctat agatacaatg gacttatacc
    46621 ttggtaatta ttttaaagat aaaactcaac atgaagttta tgaaaattac tatctttatt
    46681 tatataatat tgttaagaat tataaagatt actctgtctt aggtcattta gatttgatta
    46741 aaaggtatgc accttatgac actattttag atgatagact attttctgat ataattgaag
    46801 aaactcttaa acaagctatt tatgatggca aaggtatcga gataaatacc tcttgttata
    46861 gatataatct tccagaccta accccatcta agtatatact tcaaatgtac aaagacttgg
    46921 gaggggaaat cataaccaca ggttcagatt cacatcatat aagtcaagtt gcatgtgagt
    46981 ttgactacat atattcatta ctaaaaaata tgggatttaa atatgtatct aaatttaata
    47041 aattgaaacc agaatttata aaattataaa tttttacacc tccttgtgaa tatatttaaa
    47101 agataaggag gtgatttttt tgaaagtacc ttatttagta ctatcaacag ttattttatt
    47161 tatatccaat tttattgtaa gaatatttgg ttttttatat aaaatattct tatcaagagc
    47221 actaggtgaa acaggtttag gtatctatca tatgattttt aactttttga tgatttgctt
    47281 agctgttaca acaacaggta tacctactgc acttagttgt ctagttgcaa agagaaaagc
    47341 tttaaacgat agacacaata ctaatgcttt atttatatca actttatata tatcattttt
    47401 cgtagcacta attatatcta tagttgcatc tttcaatagt tcttttttat cacttaaatt
    47461 tttaaaagat gctaaattaa atctatttat actagctgtt tgtcctgcta tagttataat
    47521 aactctttca aacgtactaa gaggatatta ctatggcata aagaatgtaa aaatccctgc
    47581 tattggacaa atcattgaac aaattggtaa aatattattt gtttttttac ttgttatgta
    47641 cataaataat aaatctatga attgttatat agctctatta ggtatatcga ttggtgagtt
    47701 gagtaatatt atatttatgc ttatttgctt atggcgagat tcatcatttg acaataggta
    47761 tattataagc ataaaagatt tttataattc ttctatggaa acattaaaaa tgtctattcc
    47821 cataacttgt aatagaatgt caaacatact tttgcaatct ataagttcta tgatgatacc
    47881 atcaagatta gcattatcag gaatgactta tcaacaatct ttaagtatgt atggtattgt
    47941 cagtgggatg gtaatgcctt ttatattttt accttttact gttggttcag cgcttattgt
    48001 aaatttaata ccaactattt cacaagaaat ggctctaaga aagcgtaaga gtgtaattaa
    48061 aaaaatcaaa tactccatac ttcttacttt atttgtaggc atactatcat caatattttt
    48121 ttatttcttt ggaaaagatt tatgtatatt agtatttaaa aatacacttg caggagaata
    48181 tctaaaagct atgtttttag ttccactatt tatgtcccta aatcaaactc tatctggcat
    48241 tttacattca ataagaaaag aattagcttc aagtataaat actattactg gcatgttaat
    48301 tcaactaata gctttatatg tatttcttcc aattccagga cttaatattt atgcatacat
    48361 atacacaatg acgatagtat caatctttac ttgtctatta catactatag tgctttttaa
    48421 atcactaaaa tctatacgct agttataaaa acgtgaaggc taccaatgta aattatacac
    48481 tttggtagcc ttccttagat gatttttatt tataatttaa actgtctata tatctaccta
    48541 tctatctgtg gcaacttggc tttccacttc catgattagg tccacattgc gaaaaatcac
    48601 atactttaga gaaagtatca acaagtcttg gttggattgg atttggtgat gctaaaacta
    48661 ctatttgttc acttattaat gtgctaactg tttcttgtaa gcaaagtact aaatctatat
    48721 catttggtat aattaattca tagaaaggtt cacaacattc gttgcattct aattttaata
    48781 tagattttcc tatacaatct acagttaagt tagtttgata ttcttgtctt aaagtaacag
    48841 ctcttccgtc agctggcaag catattggtg ctgaaagtgt gttaaaagta aattctacat
    48901 aatcaactct tctcttacat ccacctcctg ctctaactgc tgggaaagct aatgcaacga
    48961 actctgtaca tccacatcta cctcttagct ctagtactaa attacgaact gctacagtta
    49021 atcctctttg tttataagct actgatttgc cttttccttg tctacagcac tcagagtttc
    49081 tttctccaca aactgcaaat tcaaaagttg tttcacagtt tcttcctact atatctccta
    49141 atgggtctaa atcgaaatct tctaaagttg tcattcctgt gtctataacg attccatcat
    49201 tacttaagca tattttttct atagttacac ttgcttgacc tgcacttgga actgaaccaa
    49261 atacttctac tacttctgtt tcaaaagtta atggctctcc atttggtcct gttgcatctg
    49321 taaatgtttg gaattgcata gtatcaaata ttctattagc ctctattgca aatggcactg
    49381 catttgatac agagaagttt cttggagata taggattaca gcattctggt ccacatttgt
    49441 tttcaaaaca ttcatctgaa tccatttcac atggttcaca atcacattta caattgtcat
    49501 ggcatccaca attgtcatca catggattag gtttgcatgg attgcatggg ttaggtttac
    49561 atggattaca tgggttgcat ggattgcaat catcatggca ctcacactta ttatcagtat
    49621 tacatccatt atgatggtgt gattttttaa aacctctcat gtcttctgaa tttatttcat
    49681 ctgtatacac ttcttcttca ttcattgtag acattggctg attcatcatt cttcttttat
    49741 tttttttata atcttgcatt tttcattgcc ctccctgtaa gcgattctta ttaagtcttt
    49801 ggctttttga agcctaatta atactatgct tttataatct taattgttac ttgctttttg
    49861 aatttatcaa taattaggcg tatttagtag aaaaatgcat attaataaaa aaagtactac
    49921 tatttgaaat agtagcactt tttttgtccc gaaacaccgg tggctaagta aattcacacc
    49981 ctaggcactc ccaggtgggt gacttgccag taacctttaa cgtccaaact aactaggcat
    50041 gttataattt accagacacg gtctcccgta tttcatttgt aggttgaatt taaaaaagcc
    50101 ttatcgtatg tttcaggatt tcatattatt attatataat aaattttgaa ttttttaaag
    50161 tggttttata tagtaattga gataatctta ttctatcatt actttttctt ccttttttct
    50221 ttatgtattt agttaattat cgttttttct tagctctagt tttagtattt ttttcattat
    50281 aattgtattc ggattcttgc tcattaatgt tgtatagttc ctcatcgtat actatatctt
    50341 catcattttc tatattttca tagcctgtgt tcttatcttc tactaattca tcttcattat
    50401 cctccatttc accttcatct gttaactcat catcatccaa ttctttgtct tcacaatcta
    50461 attcttcgtc ttcataattt aactcatcat tattgtcttc atcatcatta ttactatcat
    50521 cacctcttaa aagacttcct aacaatttga gattattcat actaccaaga ttgtttatat
    50581 ttgccaaaga gctcaaacta aatccacctt caccaaagtc atctggtaaa aataattctg
    50641 caaataaatc tatcttcttt ttcattccta tagctttttc aactgaattt tttctttttt
    50701 ctgggaatac atctataatt tcctttatca tcatattctt tttatcatca ctatccattt
    50761 ttctgaaaaa atccgtttca tcttctgtat ctaccatatc tatattattt aattttttta
    50821 ttcctctaac taaatctaaa acactttcta tttttattag tatttttctt tcatctcttg
    50881 acatgtattt tttagttctt gttattattt ccattcccat ttctaaatct tcttcactta
    50941 aatccatgag ccttaaacct ctttttatat catttgcatc aaaccttaaa ttgccatgtg
    51001 tagactgtga aagtttatca tttgcatttt ttttgttttt tctaactcta ttatttcctt
    51061 ttgatttatt tttatcatca aatatagaga tattaccatt actcaatgca atcactccaa
    51121 tcaaaaatag taagttattc aagacaattc ccccttatat tatataattg ccctatatta
    51181 aagtatgttt ttcatatttt aatgttccat ttttaaagct cagtgcatat atattatcta
    51241 taagaatttt tatttatatt atatatcact cacaattcac cacttcaata ttatttctat
    51301 atataaatag tatgaatata attcaatata tatttataga aaggggtttt tttatgaaaa
    51361 aattagatac agagtcatta gaaaaaatgg ctaaacaaaa aaatattgac aaggataaaa
    51421 tagaaaagat ggctgatagt tacaaaggaa aatcagaaaa tgaacttatg gaggaactta
    51481 taaaaatagg taagaatcta gatggcagag atgaagtagt atcaaaattc aaagcatttt
    51541 tagatgaaaa tcaacgcaaa aaactggata atataatgga aaaaatctca gatgcagaaa
    51601 accaaagaga tactaaacca gctaaaacta agaaggctaa acctactaaa ggtaagtcaa
    51661 attcacctgc accaaaaaat acaccaggta gtcaacaaaa aaaatctaaa agtttattta
    51721 agaaaactaa aaagtctaat cctcatgaat agataaaaat aaactattta agcaattaga
    51781 taattatttt tagttaatct attattaaat ttgatattat tgacataaaa aatcaggtag
    51841 tgcctaaggc actacctgat tgattttaat agttttattt ttttctttct gtaacacttt
    51901 ctctatcaac aattctataa ggtaactcta ttttcttatt ttcaattaat tccttattta
    51961 ttaattttat aaccattctt attgctactg ctcccatatc gtatagaggt tgatatactg
    52021 tagtaagttt aggtctatac attctagcta tcttaacatc attaaatcct gcaactgata
    52081 tgtcttctgg aaccttatat ccagcatcac atatagcatt tatagcacct atggcagcct
    52141 catcacctgt tacaaaagcc gcatgaggta ttattccatc atctaatagt tctttcatac
    52201 tattgtatcc actctcataa tcagttccac catacttaat caaactcttg tctaattcta
    52261 tattgttatt tgaaagtgct ttctcataac cagcaagtct ttccatttct aaaacagtat
    52321 catctttact agtcattata aaagctatct tttcatgacc tttctttata agatgctctg
    52381 tcatatcaaa agtagcttct tcgttgctag tacttactgt atatatatca taatttctag
    52441 cagttttact tatatatgtc gctggtattc cacaattttg tatatagttg atatgttctt
    52501 gttcaactat ccatgaaatc ataactatac cttccacttg ttttgctctc aatagattta
    52561 tactcttaag ttcttgttcc ttatcagagt atgtattcgc taaaagaata tcatagtcat
    52621 acattttagc aacctcttct atcccattta acacctcatt aacaaaagaa tctgaaactt
    52681 ctggaactat tacccctatt aattgactct tctttgttac taagcttcta gcaagtggat
    52741 ttggtatata tccagtctct tttataacct ctaaaacttt ttgtttgact tcatcagtta
    52801 caggttttga atcatttata actctagata cagtagatat tgacactcct gcttgtttag
    52861 caacatcttt tatcgttata ttgcctttca tcttcatcct ccttatttta ctaaatttat
    52921 ctaaaactga accaaatttt tgaaggttcc tattttaact acatttatat aagtttttat
    52981 tttaccataa aagcaagtac atttaaatac attatatttt atattatacc ctatattcaa
    53041 caaatttaat aatattcatt cttaatttaa atcatattaa aaaataaaat aaaaaaatgc
    53101 tggtataaaa taccagcatt tttttattct aatgatttaa gtattagcag ccgcaaccta
    53161 cttcaccatc aaagaatcca ccacatgaac aagttattag caataatatt aagaaaggta
    53221 tccatgacat tatgtcaatg tctctccagc aatcttcaaa agctaagaat aggaagaata
    53281 gtactattat ccaccatcct cctccaccaa agcatccttc ttctccaaaa aatctatcta
    53341 acataacaaa gccctccttt ttttaataac tgtgttctta ttctaagcat ccaccacagc
    53401 agcaaagtat taataaaact attaggaatg gtatccatgc cattatgtca aattctgccc
    53461 agctatctcc aaaagctaag aataggaaga atagtactat tatccaccat cctcctccac
    53521 caaaacatcc ttcttctcca aagaatctat ctaacattat acgttacctc ctgttgatat
    53581 tttgttgagc ttttcgctcc taacatatac tatgcatttg gtttccattt tgctaatgta
    53641 tttttactat ttttttaaat tttttaattt gaactaaaaa aggtaaaacc ataacttgta
    53701 taagttactg gttttaccta tttaataatc aatatttttt agcaacaaca accatcatcg
    53761 aagttaaata ataacaatac tattaatata acccatatcc atatattgtc atcgcataat
    53821 aagtcttcta aaatgtctgt gcataatagt aagaatacta taactattat tatcaacatt
    53881 ttattcccaa aaaatccttc gcatcctcca aagaaacgtt ccatattgtc ttctaatttt
    53941 gtcataacta atcctccttg aatcattaag agactaagtt ttgtctcttt aattaaatat
    54001 atgtagtaat agacagataa gttacttcta aaaagttttt attgattagt ttatttattt
    54061 ctttttcacc aagtttattg tatctatcaa cttcatccag ataatctttt ttctcctctg
    54121 atatagtata gattaaactt tctattgatt ttattttgta ctccttatat ctttctatca
    54181 agaaggatgt ttttgaattt tctattcctt tctttacatg cattaacctt gatatgtaat
    54241 cttctttttc attagataca taactctcct tttcagctac atcttcaagg attaaattgg
    54301 tctccattac ataatcctct atacgttgca tatctaaagt tattgaatta taataaaaat
    54361 ttactgctat aagtaaaaac attcctactg aagttagtaa tagtcctaca tatttcatag
    54421 catttcctcc taagttatat ttgttatcaa taaagtttat tcatcaaata atactttatt
    54481 actacattta taaaatttta ttatttttta tagattccat attgaaaaat tcttgatatt
    54541 atattaaact aacattaaaa ttaattaaaa ctgctttagg aggcttaaaa tgtttgagaa
    54601 aataaaagaa ataatagcag aacaattagg ggtagacaat ttagatgaga taactatgga
    54661 agcatcttta atggacgact tagaagctga ttcattagat gctgttgaaa ttataatggc
    54721 ccttgaagat gaatttggaa tagaaatacc agacgaggaa gctgaaaact ttaaatgtat
    54781 tggtgatata tgtaaatata tcgaagaaaa caaatagtat cgttttgtct acaaatataa
    54841 atagtctgtg taaacttcta cataataaat tatattagag ttcgcacaga ctagattaca
    54901 tttatttaca atatataaaa tacacgctct tatatttaat ttatatacta agtatataaa
    54961 ttacttttct tcataaaacg taacattctt tattattata tctatagttc catcttgatt
    55021 ttgttttaca gtatacttca ttgggtcttc aaaatcagtt agattccctt ttatgtcaaa
    55081 accattatcc gtttttatac ttcttttttt aagctttttc tcaacccatt ttttatctat
    55141 actaaaactt tcgtcaagac ctttttcttc catatgctct ttaaagctat cctttaactc
    55201 atcatcttta attgaattat caacaaagtc atttatatca acttcatgtt tttccttcag
    55261 tgtataattt aatatacttc ttacatcttc agcttgcttt atatcattac taagagcatt
    55321 ggttatccaa ttttcagctg tatttttaaa taccttggtc ttgtacttat catcatctat
    55381 ttttttagca tttaaaaatt ctgttacaaa cttagattct gacccttctt tttcagcatc
    55441 cttatctaaa agccttagat gaaactcatc atttatacca cttagactaa tcaaagctcc
    55501 ttgcttttgt cttaaagtct ctgggatacc aatttcattt gaaaccattt gaatattaaa
    55561 tttatcatcc acaaattcaa ttgaatgagt gtacaacttt ttataatcta atttcagtat
    55621 tgctacactc ttttcatctt taatagtgta taagcaaatt gccaaatcac aagattctag
    55681 tgcagcatta attttcataa catcaaacaa gtaagatgca atttctttag aatttttcaa
    55741 aaaagtactt tcatcatata ttatttgttc acaacagttt ttaattaaat tgtcattata
    55801 atctttaaaa acaccttttc ttaaatcctc atcttttgca attcttttta tagctttctg
    55861 gaaaaaacca tctacttctt gattaacttt gccttcaaag tcatttaata ttggaacatc
    55921 actatttttg tccaaaacat gtattataaa tttatgtatt atcatatata tcaccccatt
    55981 atcttaattt cgatattaac tctttcatat cctctggtat atctgcttta aatttcaatt
    56041 tttcttttgt tcttggttgt tcaaactcta gcttatatgc atgaagtgct tgcctattta
    56101 ttagatcttc atctacataa ccatacaact catcacctat tattccatga cctatatgag
    56161 ccatatgtac tcttatttga tgtgttctac cagtttctaa gcttacttct aatacagttg
    56221 cattttctag tctttctatt actttatagt gagtaacaga tgactgtcct ctttcatcta
    56281 ttatcctctt tattgaatct tctgtaggtc tataaattgg ctcatctata gttccctcat
    56341 tttcttttat aattccatca actactgtta tatacttttt ttcaacttta ttttcactca
    56401 ttgctgtaga caaagtatga tgtgcataag cattttttgc tactataact aaccctgaag
    56461 tattcatatc taacctattt acaaatctta tttttacatt ttctttttga ttatctatat
    56521 agtaactaat accatttgct attgttttat catagtggct ttttgttggg tgaactacca
    56581 taaacggagg tttattcacc ataattatat caaaatcatc ataaattatc tgtaaattta
    56641 aatcttgagg ttcaaaattt gctttttcct cgtctatttt tacttctatt aaatcaccac
    56701 tatacacctt taaactcggt tttttatata ctccatttac taaaacactc ttttccctct
    56761 tcatttttga taatgaccta actgagaaat ttagcttgtc caataaaact tcttttaaag
    56821 tcatttcttc ttcatttgta tacgatataa gattatatct ttgattctct tttttaaaca
    56881 aacttttacc tactttcttt tatatcttcc tactttttta tatcttcaaa atcatgatat
    56941 tatatatttt acactatttt tgtttaatag ataataatta tttttaacta taaaaacctg
    57001 taagaataac aaatagttat tcttacaggt taaattcata attaaatatt taatttgtaa
    57061 atattataat ctaaaactat ctcttaacta ttaaagtagt tcccattcct ccacctatac
    57121 aaagtgtagc aagaccagtt ttagcatctc ttctcttcat ttcatataaa agtgtagtaa
    57181 gtattcttgc tcctgagcat cctattggat gtcctatagc tattgctcca ccattaacat
    57241 taactttgtt catatctata tttaagtctc ttattacagc tacagattgg gcagcaaatg
    57301 cctcattagc ttcaactaaa tctatatctt caatagtcat attagcagct tctaaagctt
    57361 ttttagttgc tggaactggt ccatatccca ttattttagg gtcaacacca gctgttccat
    57421 aagaaactat agttgcaaga ggctctattc ctagttcttc agctttttct ttagccatta
    57481 ctactagcat agcagcacca tcatttattc ctgatgcatt accagcagta actgttccat
    57541 cttttttaaa tgcaggtctt aacttagcaa gtttctccat tgtagtgcca ggcttaatat
    57601 attcatcttt atctactaca gtgtcacctt ttcttccttt tataacaaca ggaactattt
    57661 cttcatcaaa ttttccttca gcttgagctt tttcagcttt attttgactt gcaagagcta
    57721 attcatcttg ttcttctcta gttatattcc attgctctgc tatgttttca gcagtaatac
    57781 ccatgtgata gttattaaat atgtctgata atccatcttt tatcattgaa tcaacaaaag
    57841 cagcatcacc cattcttgca ccatatctcg cacttggtac taaataagga gacatactca
    57901 tgttttcagc tccaccaact aacattatat cagcatcacc taatgctata agttgagatg
    57961 ccattgaaac agatcttaat ccagaaccac aaactatatt tatagtcata gctggttttt
    58021 ctactggtat tcctgctcct aatgctattt gtcttgctat attttgtcca agacctgctg
    58081 taagtactcc ccctaaaaga gattcatcta tcatatctgg agttatgtta gctcttttta
    58141 tagcttcttt agctgctgtt acccctaact ctaccgctga aactgattta aatgctcctc
    58201 caaaacttcc tactgctgtt ctagctgcac tggcaattac tacttctctc atattcatta
    58261 cccccattaa attagatttt tcatatatcc gaaattgttt tttaaacaat tttatttaaa
    58321 cttttaattc ccaaattctt atttattata atcatagaat cctatcttag tttttcttcc
    58381 taattgatta gctctaacca ttttagctaa aagtggatga ggtctatatt tagtatctcc
    58441 aaactcagta tataaaacgt tcattatagc taaaacaaca tctaatccaa ttaaatcacc
    58501 taatgctagt ggtcccattg gatggtttgc tcctaatttc atagcttcat ctatttcttc
    58561 tttacttgca acaccatctg catatatacc aacagcttca tttatcatag gtataagtat
    58621 tctatttact acaaatccag gagattcaga tacatctact ggtactttat tgatactctt
    58681 agataattca aatactgtat caaaagtaac ttttgatgtt aactgaccac ttataacttc
    58741 aactaatttc atcataggaa ctggattaaa gaaatgcatt cctataactt tatctggtct
    58801 cttagtagaa gaagctattt ctgttataga taatgatgaa gtatttgttg ccaagatagt
    58861 atcttcttta cataattcat ctagtaactt gaaaacatct ttctttatat tcatgtcttc
    58921 tacagatgct tctattatta aatccatatc ttttaaatct tcatagttag tagttgaact
    58981 aacatgactt aatatttctg cttttgtagc ttcatccatt tttcccttag taactaactt
    59041 agttaaattt ttatctaata aagctaaaca tttatctata gcaccttgag ttctactctt
    59101 taaacataca tcatgtccac aacttgcaaa agtttgtaca ataccacttc ccatagttcc
    59161 actacctatt acagctaatt tcatagttat aacctcctgt tattttagta tttatattct
    59221 gttaactaga ttaaaatact atttaatcta gttaacagca aatatatttt taattagatt
    59281 tttacccttt tataaagtta gcttctctct tttcaacgaa agctgacatt ccttcttttt
    59341 ggtctttagt tgaaaaacaa agaccaaata aattagattc tatatctatt ccagtattta
    59401 tatcagtttg agcaccaagt tgtattgctt ctttagagta tctaactgca agctgagcat
    59461 ttttagctat tatcttagct aatttctcaa cttcttctat taaaatgtct ggctcaacga
    59521 ctctatttac tagccctatt ttttcagctt catcagcttt tataacttga cctgtaaaga
    59581 ttaattcttt tgcttttgcc attccaacca atcttgtaag cctttgagtt cctccatatc
    59641 ctggagttat tccaagagtt acttctggct gaccaaattt agctttagca gatgcaattc
    59701 ttatatcaca tgccattgca agttcacatc ctccacctaa agcaaatccg tttacagcag
    59761 ctatcactac ttttttacta ttttctattt ctccaaaagc ttttgctcct aagatactaa
    59821 aatctttagc agctacagca tctaaatctt tcatgtatgc aatatctgct ccagctacaa
    59881 atgcctttcc ttcccctgtc aatattacaa catcaatagt ttcatcatta ttaatatcta
    59941 caaatacttc ataaagttct tctaaagtct ttgaatttat tgcattaagg gctttaggtc
    60001 tattcatttt cactgtacat atatttccat ctacttcaac agctacattc tcataaactt
    60061 taacatcact tgtactcata ataccctccc caagaaatta gttagctaaa acttcacctt
    60121 tttcttttgc aacacttaac tgactgataa gttctggaag cactttatga acatctccaa
    60181 ctataccaac atcagcatat ttaaatattg gagcttctgg atttttattt atagcaacta
    60241 taaactcagc atcttccata ccagctatat gttgtattgc tccagatata ccacatgcta
    60301 tataaaggtc tggtcttaca gttttaccag tttgaccaac ttgtcttgct ttatctaacc
    60361 aacctgcatc tatagtggca cgagagccag aaacttctcc acctataatt tcagctaatt
    60421 cataaagtat gtctaagttt tcttttccac ccattccacg tccagcagaa actaatatct
    60481 tagcatcttc tattttaact tgttttttag cttcttttat tacttgtaca acttgaacta
    60541 atttatcagc atcattaaat tctaccttga aacggttaat tacagcttct ttagtttcat
    60601 caggttcatt tttcttcata acccctggtc taactgtaga catttgaggt ctgaaatctt
    60661 tacaaactat tgttgccatt atatttccac caaaggcagg tcttgtcatt aataataatt
    60721 ttgtatcttc agctactgca agacctgtac agtcagcagt aagacctgta tgtattctag
    60781 cagaaactct aggcgctaaa tctctaccta ttgaagttgc accaaataat acaactatag
    60841 ggtcagctgc ttttattgct tcataagctg cttttgtata tggttcagtt gtatacactg
    60901 ctaaagcttc atcatctact actattacct catctgcacc atagtgtgct aatgtatcta
    60961 ttaaaccttc taccttacta cctaaaagta atgcagaaac ttttgtatca taatcttttg
    61021 ctatttctgt agcctttcct agtaattcta aagaaacagt ttgaattaca ttttctcttt
    61081 gttctattac tactaaaacg ttacccattc tttatccccc cttaaattat atgatatact
    61141 tctcttttaa tttatctatg ataattccag ctgatgtttt cgcatcttca ttgtatattg
    61201 taccagctgg tttaactgat tttgtaaatg atttaaatac actagttgga gaacctttaa
    61261 gacctaaatt agaagggtca acttctatat cttttacagt ccatgtttct actacatcat
    61321 tttcgaaagc atcatatatt cttccaactt tcatgtatct tggtgtgttc atatctttaa
    61381 gagttgttat aaggcatggc attttaactt ttaagtcatg gcaacaatct tcaaattgtc
    61441 tttttactaa tacatattca ccttcagttt ttatttcttc agcatatgtt attgatggaa
    61501 gatttaaatg ttcagctatt tgaggtccaa cttgtgcagt atctccatct atcgcctgtc
    61561 ttccagctat tataatatca aaatctatat tttttaatgc tcctgctaat gctgatgaag
    61621 ttgcccaagt atcagcacct gcaaatgctc tatctgttaa taatatacct ctatctgcac
    61681 ccattgctaa agcttctttt aaagccatat ctgcttgagg aggtcccatt gttataacag
    61741 ttacatgagc acccatttct tcttttaatt ttatagcttc ttctaaacct gctttatcat
    61801 cagggtttat tatacttggt actccatctc taattaaagt acctgtatta ggatctagtt
    61861 taacttctgt tgtatctgga acttgtttta tacaaacgac tatattcata aatccttcct
    61921 ccatatctat tttaatatcg acctattgtg tatattttaa tatttatcta ttttaataat
    61981 tttcctgaaa taaccattct ttgaacttca ctagttcctt catatatttc agttatctta
    62041 gcatctctca tcattctttc tactggatag tcacgagtgt atccatatcc tccatgaagt
    62101 tgtacagctt ttgtagtaac ttccatagct gtttcagctg caaataattt tgccattgct
    62161 gcttctactc cataaggttt tcctaagtct ttatttatag ctgcttgata tacaaggtgt
    62221 ctagccgctt gtaccttaac ttccatatca gctaattgga attgtgtatt ttggaatttt
    62281 gataatggtc taccaaattg tactctttct tttacatatt taacagtttc atcaagagca
    62341 ccttgtgcta aacctaaagc ttgtgcagct ataccaattc taccaccatc aagagtagac
    62401 attgctatct taaatccttg accttctttt ccaagtaaat tttctttagg tattctgcaa
    62461 tcctcaaata ttaattcact cgtagctgaa cctcttatac ccattttctt ttctttaact
    62521 ccaaagctaa acccaggagt tcctttttca actataaatg ctgatattcc tttgttcccc
    62581 ttagatttat cagtcattgc cattactaca tatatgtcac cagctattgc gtttgttata
    62641 aatatttttg agccattaag tatgtattca tccccgtcta aaacagcagt tgtttgttgg
    62701 ccagacgcat ctgtaccagc attaggctca gtaagaccaa atgctcctaa tttttctcca
    62761 cttgctagtg gtcttaagaa tttttgtttt tgttcttcat taccatattg atatataggc
    62821 catgagccaa gagatgtatg agctgataat ataactcctg tagtaccaca aactctagac
    62881 aattcttcaa ctgccattat atatcctaca gtgtctccac cttctccacc atattctttt
    62941 ggatatggta tacccatcat tcctgctttt gccatttttt ccactgtttc ataaggaaat
    63001 ctttcttctt catcaagttc tgttgctaaa ggtttaactt cattttcagc gaagcttaca
    63061 tatagctctt taagcatctg atatttttta gaatttaaat ccataatagc ctcctcagta
    63121 ttttataaat attttatttt catttattaa ttaaaatctc ggcaacatct ataataaggt
    63181 atttgaatat acgtcgagaa atttataacc ttaatcaaac tacgctctat atttatatgt
    63241 gattatacta accaattatg acattaattt aatcataaat tgttaattta aaatcaacta
    63301 tacttaaaaa aaatctcgct ttttggaatt ttgtgaaatt tcaagttaga ataagatttc
    63361 tttcacactg gttacagata aaataatttg tttacgatat ttatattagg gtatattaaa
    63421 cattaaatat tgtctgtaca tctgtttttt agccatgtac agaagatttt tatcgtataa
    63481 aaattatttt aatagagcta aataatcgta tttttatctg actatttttt atttttaaat
    63541 atcttacatc tataatagta tcactcaaag gataattttt caataatttc taattatttt
    63601 tttaacaata tttattacat tttttccact tttttgtcat ttttttattg tttcgaaaaa
    63661 aaatcagaat tttttaactt tattttggat tattagttta aattagattt tcctcttaga
    63721 tttataaata tttacacaaa aaaactacct ctgtatagat tgtatacaga ggtagttttt
    63781 tactaattcc tactatagtt tactatattt ttttaccaat tcttacaaac atctacccat
    63841 ttttcacact tagaaaactt ctccatttct tcacatgtaa gttctattgc agagttacta
    63901 ctgccacatg caggaaatac tgtatcaaat cttttcattg aatcatctaa atagactttt
    63961 atagaatttt ttaaaccaaa tggacagact ccaccaatag catgtccagt aaactccaag
    64021 acttcttctg gtgtcaacat tttagcctta caattaaatt cagccttata cttcttatta
    64081 tcaatcttag catcaccagc tgtcacaatt aaaattgcat catctcctat tttaaatgag
    64141 agtgtcttag ctattcttgc tggaataacc ccagctgctt cagctgcaag ttccacagta
    64201 gcactagatt gttcaaattc taatatactg tcttccttgc caaattgttt aaaatactct
    64261 cttacattac ttattgacat taaatatccc tcctagtctg aacttttata aatttcatat
    64321 tattcttaat tatatctata attgatttat ttttcaaatt attccatgat attagcattc
    64381 catgtatcat tattttttcc aactactaga taaagtcctt ttgccattgg aatattgaca
    64441 atatattctc ctttatactc tacactcata ccagattcat taaagtacct ataatcaaaa
    64501 tttagtcctt ttggaattac aaaatcaaca taaccttgat tatttggttt tattccatat
    64561 ttcagagttc tttcagttat agaatttttt tcattaaata aatctacatt tacatatgca
    64621 gaacacttat tcattccctt tttattttcc acacaatttt caaacttcat tttagcactg
    64681 tttaatttat aactatattt actattattg cttattttaa atcttacaat agtaccaaca
    64741 tctctacttc tcctgctatc aaaatcaaaa acaatttcct ctaaatgatt tatttcataa
    64801 tctttgacac ttaccatatc tctatatact aatttattta tagctatgaa tagtgtaaaa
    64861 atccccaaaa taaaaaatga aacttttata aacttcttca tttttagtct ccttatattt
    64921 tgtgctattg tgctataata ttaatcaaaa ttttaa
//
LOCUS       DAEMGG010000007        64562 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEMGG010000007 DAEMGG010000000
VERSION     DAEMGG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.007"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            186..1382
                     /locus_tag="KPK24_000451"
     CDS             186..1382
                     /locus_tag="KPK24_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG2298160.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1475..1999
                     /locus_tag="KPK24_000452"
     CDS             1475..1999
                     /locus_tag="KPK24_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG2298161.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            2024..2200
                     /gene="rpmF"
                     /locus_tag="KPK24_000453"
     CDS             2024..2200
                     /gene="rpmF"
                     /locus_tag="KPK24_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG2298162.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2407..2964
                     /gene="fapR"
                     /locus_tag="KPK24_000454"
     CDS             2407..2964
                     /gene="fapR"
                     /locus_tag="KPK24_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG2298163.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2975..3997
                     /gene="plsX"
                     /locus_tag="KPK24_000455"
     CDS             2975..3997
                     /gene="plsX"
                     /locus_tag="KPK24_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG2298164.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            4023..5009
                     /locus_tag="KPK24_000456"
     CDS             4023..5009
                     /locus_tag="KPK24_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG2298165.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5118..6047
                     /gene="fabK"
                     /locus_tag="KPK24_000457"
     CDS             5118..6047
                     /gene="fabK"
                     /locus_tag="KPK24_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG2298166.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            6063..7013
                     /gene="fabD"
                     /locus_tag="KPK24_000458"
     CDS             6063..7013
                     /gene="fabD"
                     /locus_tag="KPK24_000458"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG2298167.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQVDLKYSACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQDTVPTGVGAMAAILGLDRSILENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLREAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            7013..7762
                     /gene="fabG"
                     /locus_tag="KPK24_000459"
     CDS             7013..7762
                     /gene="fabG"
                     /locus_tag="KPK24_000459"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG2298168.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7816..8040
                     /gene="acpP"
                     /locus_tag="KPK24_000460"
     CDS             7816..8040
                     /gene="acpP"
                     /locus_tag="KPK24_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG2298169.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            8069..9307
                     /gene="fabF"
                     /locus_tag="KPK24_000461"
     CDS             8069..9307
                     /gene="fabF"
                     /locus_tag="KPK24_000461"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG2298170.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9533..11251
                     /locus_tag="KPK24_000462"
     CDS             9533..11251
                     /locus_tag="KPK24_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2298171.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKHDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11282..13282
                     /locus_tag="KPK24_000463"
     CDS             11282..13282
                     /locus_tag="KPK24_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2298172.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSELSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13633..13878
                     /locus_tag="KPK24_000464"
     CDS             13633..13878
                     /locus_tag="KPK24_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG2298173.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13913..14638
                     /locus_tag="KPK24_000465"
     CDS             13913..14638
                     /locus_tag="KPK24_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG2298174.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14660..15973
                     /locus_tag="KPK24_000466"
     CDS             14660..15973
                     /locus_tag="KPK24_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2298175.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVVMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYDENLKKIV"
     gene            16179..16718
                     /locus_tag="KPK24_000467"
     CDS             16179..16718
                     /locus_tag="KPK24_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2298176.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16807..18792
                     /locus_tag="KPK24_000468"
     CDS             16807..18792
                     /locus_tag="KPK24_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBG2298177.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            19016..19984
                     /gene="spoIIIAA"
                     /locus_tag="KPK24_000469"
     CDS             19016..19984
                     /gene="spoIIIAA"
                     /locus_tag="KPK24_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG2298178.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19974..20495
                     /locus_tag="KPK24_000470"
     CDS             19974..20495
                     /locus_tag="KPK24_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG2298179.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20512..20709
                     /gene="spoIIIAC"
                     /locus_tag="KPK24_000471"
     CDS             20512..20709
                     /gene="spoIIIAC"
                     /locus_tag="KPK24_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG2298180.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20725..21111
                     /gene="spoIIIAD"
                     /locus_tag="KPK24_000472"
     CDS             20725..21111
                     /gene="spoIIIAD"
                     /locus_tag="KPK24_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG2298181.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21196..22401
                     /gene="spoIIIAE"
                     /locus_tag="KPK24_000473"
     CDS             21196..22401
                     /gene="spoIIIAE"
                     /locus_tag="KPK24_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG2298182.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22461..23078
                     /locus_tag="KPK24_000474"
     CDS             22461..23078
                     /locus_tag="KPK24_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG2298183.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            23090..23644
                     /locus_tag="KPK24_000475"
     CDS             23090..23644
                     /locus_tag="KPK24_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG2298184.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23671..24360
                     /locus_tag="KPK24_000476"
     CDS             23671..24360
                     /locus_tag="KPK24_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG2298185.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSYSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24470..24832
                     /locus_tag="KPK24_000477"
     CDS             24470..24832
                     /locus_tag="KPK24_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG2298186.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24917..25423
                     /gene="nusB"
                     /locus_tag="KPK24_000478"
     CDS             24917..25423
                     /gene="nusB"
                     /locus_tag="KPK24_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG2298187.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25407..26477
                     /locus_tag="KPK24_000479"
     CDS             25407..26477
                     /locus_tag="KPK24_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG2298188.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26474..27679
                     /locus_tag="KPK24_000480"
     CDS             26474..27679
                     /locus_tag="KPK24_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG2298189.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27681..27896
                     /gene="xseB"
                     /locus_tag="KPK24_000481"
     CDS             27681..27896
                     /gene="xseB"
                     /locus_tag="KPK24_000481"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG2298190.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27899..28786
                     /locus_tag="KPK24_000482"
     CDS             27899..28786
                     /locus_tag="KPK24_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG2298191.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28816..29253
                     /locus_tag="KPK24_000483"
     CDS             28816..29253
                     /locus_tag="KPK24_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG2298192.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29287..31152
                     /locus_tag="KPK24_000484"
     CDS             29287..31152
                     /locus_tag="KPK24_000484"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG2298193.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31285..32097
                     /locus_tag="KPK24_000485"
     CDS             31285..32097
                     /locus_tag="KPK24_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBG2298194.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32117..33796
                     /gene="recN"
                     /locus_tag="KPK24_000486"
     CDS             32117..33796
                     /gene="recN"
                     /locus_tag="KPK24_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG2298195.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            34011..34877
                     /locus_tag="KPK24_000487"
     CDS             34011..34877
                     /locus_tag="KPK24_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298196.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDIDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34970..35461)
                     /locus_tag="KPK24_000488"
     CDS             complement(34970..35461)
                     /locus_tag="KPK24_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2298197.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35522..35962)
                     /locus_tag="KPK24_000489"
     CDS             complement(35522..35962)
                     /locus_tag="KPK24_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2298198.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36123..37181
                     /locus_tag="KPK24_000490"
     CDS             36123..37181
                     /locus_tag="KPK24_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG2298199.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGGAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37323..38147
                     /gene="spo0A"
                     /locus_tag="KPK24_000491"
     CDS             37323..38147
                     /gene="spo0A"
                     /locus_tag="KPK24_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG2298200.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38560..39681
                     /locus_tag="KPK24_000492"
     CDS             38560..39681
                     /locus_tag="KPK24_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298201.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39699..40574
                     /locus_tag="KPK24_000493"
     CDS             39699..40574
                     /locus_tag="KPK24_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG2298202.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40598..41290
                     /locus_tag="KPK24_000494"
     CDS             40598..41290
                     /locus_tag="KPK24_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2298203.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41298..42122
                     /locus_tag="KPK24_000495"
     CDS             41298..42122
                     /locus_tag="KPK24_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG2298204.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42127..43194
                     /locus_tag="KPK24_000496"
     CDS             42127..43194
                     /locus_tag="KPK24_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG2298205.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43196..43732
                     /locus_tag="KPK24_000497"
     CDS             43196..43732
                     /locus_tag="KPK24_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2298206.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43800..44405
                     /locus_tag="KPK24_000498"
     CDS             43800..44405
                     /locus_tag="KPK24_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG2298207.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44417..45331
                     /locus_tag="KPK24_000499"
     CDS             44417..45331
                     /locus_tag="KPK24_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2298208.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45373..46545
                     /locus_tag="KPK24_000500"
     CDS             45373..46545
                     /locus_tag="KPK24_000500"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG2298209.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46592..47404
                     /locus_tag="KPK24_000501"
     CDS             46592..47404
                     /locus_tag="KPK24_000501"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG2298210.1"
                     /translation="MFEKIAQSSKFINSKSDIKPKIGLILGSGLGDLANDIEDPVAIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47608..48933
                     /locus_tag="KPK24_000502"
     CDS             47608..48933
                     /locus_tag="KPK24_000502"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG2298211.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49146..50225
                     /gene="mltG"
                     /locus_tag="KPK24_000503"
     CDS             49146..50225
                     /gene="mltG"
                     /locus_tag="KPK24_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG2298212.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYMDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50397..51071
                     /locus_tag="KPK24_000504"
     CDS             50397..51071
                     /locus_tag="KPK24_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG2298213.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            51058..52305
                     /locus_tag="KPK24_000505"
     CDS             51058..52305
                     /locus_tag="KPK24_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG2298214.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52377..54041
                     /locus_tag="KPK24_000506"
     CDS             52377..54041
                     /locus_tag="KPK24_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG2298215.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54138..54422)
                     /locus_tag="KPK24_000507"
     CDS             complement(54138..54422)
                     /locus_tag="KPK24_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2298216.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54445..55962)
                     /locus_tag="KPK24_000508"
     CDS             complement(54445..55962)
                     /locus_tag="KPK24_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2298217.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(56087..56908)
                     /locus_tag="KPK24_000509"
     CDS             complement(56087..56908)
                     /locus_tag="KPK24_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2298218.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIKIKD
                     YEVAYNLLSKDSKENVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            57099..58126
                     /locus_tag="KPK24_000510"
                     /pseudo
     CDS             57099..58126
                     /locus_tag="KPK24_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
     gene            58198..58350
                     /locus_tag="KPK24_000511"
     CDS             58198..58350
                     /locus_tag="KPK24_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298219.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRTKSDLRVEFKF
                     IFKSKK"
     repeat_region   59004..60152
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=59004..59032
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            complement(60439..60663)
                     /locus_tag="KPK24_000512"
     CDS             complement(60439..60663)
                     /locus_tag="KPK24_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298220.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRE
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            61342..61497
                     /locus_tag="KPK24_000513"
     CDS             61342..61497
                     /locus_tag="KPK24_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBG2298221.1"
                     /translation="MALEVKIEVKIIKDVVILNITHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     gene            61743..61994
                     /locus_tag="KPK24_000514"
     CDS             61743..61994
                     /locus_tag="KPK24_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298222.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            62636..62845
                     /locus_tag="KPK24_000515"
     CDS             62636..62845
                     /locus_tag="KPK24_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298223.1"
                     /translation="MMGLKLKRRYKNVLIYINIRTIININCKIKFIELYLTFNELKNK
                     LANNEKTTVINENNKAFSGLVDFSN"
     gene            complement(63214..63597)
                     /locus_tag="KPK24_000516"
     CDS             complement(63214..63597)
                     /locus_tag="KPK24_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298224.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            63724..64053
                     /locus_tag="KPK24_000517"
     CDS             63724..64053
                     /locus_tag="KPK24_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298225.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVF"
     gene            64250..64459
                     /locus_tag="KPK24_000518"
     CDS             64250..64459
                     /locus_tag="KPK24_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298226.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFESIATLVRGGSSWSFNFIV"
BASE COUNT        25806 a         6721 c        11565 g        20470 t
ORIGIN      
        1 tataatataa aaaaaaagta tatactttta tattaaaagg tgatatatta tttatgaaaa
       61 tcgtttaatg tatacaataa tatatctata acaaaataat aaaagataga gttttattta
      121 gacatttatt acaaatcaaa atttattaca tattatttat aaaaaaatct aaggaggaaa
      181 taataatgaa aatattagta ttaaactgtg ggagttcttc attaaaatat caattaatag
      241 atatgaataa tgaagaagtt ttatgtatag gattggttga aagaatagga atagaaggtt
      301 ctatattaaa acatgaaaaa gcaggtagag atgataaata cgttgttgaa caaccaatga
      361 aggaccacaa agatgcaata gcattagttt tagaagctgt tgctcatcca gaatttggtg
      421 cagttaaaga aatgaaagaa atagatgcag taggacatag agttgtacat gctggagaaa
      481 aatttgcaac ttcagtagta ataactccag aagtagaaga agctttaaaa gaatgtatag
      541 atttagctcc acttcataat ccagcaaaca taatgggaat agatgcttgt aaagctatac
      601 ttccagatgt accaatggta ggtgtatttg atactgcttt ccatcaaaca atgcctaaat
      661 cttcatactt atatggttta cctcatgagc tatatactaa atatggggta agaagatatg
      721 gattccacgg aacttctcat aattatgtat ctcagagagc agcagagata ttaggaaaag
      781 atataaaaga tttaaaaata gtaacttgtc acttaggaaa tggtgcttct atagctgctg
      841 ttgatggtgg aaaatgtgta gatacatcta tgggattcac tccattagaa ggattaataa
      901 tgggaactag atgtggagat atagacccag ctatattacc attcttaatg agaaaagaag
      961 gtttagatgc tgatggattg gataaattaa tgaataaaga atctggagta tatggaatga
     1021 ctggaatttc tagtgacttc agagatatag aagatgcagc taaaaatgga gacgaaagag
     1081 ctcaagctac attagaagcg tatgttaaaa aagtacaaaa atatataggc gcttatgctg
     1141 ctgaaatgaa tggattggat gttgtagtat ttactgcagg cgttggtgaa aatggaaagg
     1201 ctataagagc agatattgct tctaacatgg agtttttagg aatgaaatta gataaagaag
     1261 ctaatgatgt tagaggaaaa gaaacagtaa tatctactgc tgattctaaa gtaaaaatgc
     1321 ttttaatacc aactaatgaa gaattaatga tagctagaga tacattaaga ttggtaaaat
     1381 aattaaatta aacctatgcc tatcaagtaa aaatacttga caaacaaatg ctagatttgt
     1441 ataataaatt tggtgatatg ttgaggtgaa cttaatgaaa gttagcatag aaaaaataaa
     1501 tagaaaagaa actgacaaaa tagacttgaa tttttgtgaa aaaattgata ctattagtta
     1561 ttgtgatgag atttacaaat tagtatcacc tgtaaattta aaagggaaag tgtcaaagac
     1621 taataaaggt ttgtatctag atatagatgt caattttaca attgttgaca attgttcaag
     1681 atgtcttaaa gaagtagaaa taccactaga gtattctata caaggttttt tagtaaaaga
     1741 agaagattat gatgaagatg agtttgagga atttgatcct tttatattcg atggtgaaga
     1801 aatcgacctt attgatataa tagaacagac attagatttt aatgtacctc ataaagttct
     1861 ttgtagtgaa aactgtaaag gtctttgtca agtatgcggg gcaaacttaa atgaagaaga
     1921 gtgttcttgc agtgaaatta caaacgatga ggaatacata gatcctcgtt ttgctaaatt
     1981 aaaagattta tttaattaaa ttttgactaa ggaggtggcg taaatggcag taccaaagcg
     2041 taaaacgtct aaatcaaaca caaaaatgag aagagcagct aactcaaaga tggaagcaac
     2101 aggatttgta agttgcccac aatgtcatga gccaaaatta ccacatagag tgtgtccaga
     2161 ctgtggatat tataaaggta aagaagttgt gtctaagtaa tagcttctaa aaagcatgta
     2221 gagtatactc tacatgtttt ttttgtacat ataaaaaaat aagatgattt actagagaaa
     2281 ataagtggat tttatgcata ttaaaaaata gaaaaataat aatataagta ttgaaaattt
     2341 ttgtgtgtta ctatatacta ctattagtag ctggtactaa ttacagctat aaaatttgga
     2401 gggaacatga aaaagaaaag taaagcccaa agacaaaaag agcttataga tatgctaaag
     2461 acagatcctt tttatactga tgaagaatta tcaagtctgt ttgatgtaag tatacagacc
     2521 ataagattag atagaatgag tctaaacata ccagagctaa gagaaagagt caaatcaata
     2581 gcagaaactc agagttcaaa agtaaagact ttaggagtaa aggaaattac aggagaaatc
     2641 attgatttat ctgtagggcg attaggtata tctatgttag aagttacaca ggacatgatt
     2701 tactctaaga ctaatacttt aaaagatacg tatatttttt ctctagcaga ttcattggca
     2761 atggctataa tagatgcacc taaggttatt atgagagttg caaatgtaaa aagttttaag
     2821 ttgatagaac aacaagatag acttatagca aaagcagaag tgtatagaaa tatagataaa
     2881 aagcattatg taaaagttgt tatcaacaat aaagcccaag agcaaatatt tagaggaaag
     2941 tttatctttg aagaattaga ttaggaggga aattatgaaa atagtaattg atggaatggg
     3001 tggagataat gcacctaaat ctaatgtaga aggcgcagta aatgctataa aagaatatca
     3061 ggttgattta ataatcacag gagataaaga tttacttgaa aaggaatttt caaattatga
     3121 atttgataga aataaattag agatagttca tactactgag attatagaaa acgaagataa
     3181 accagtaaag gctataagaa gtaaaaaaga ttcatctatg gtagtagcat taaatcttgt
     3241 aaaagaagga aaggcagatg ctataatatc agctggaaat acaggagcct tacttgctgg
     3301 tggattattt gttgttggta gaataaaagg aatcgataga ccttgcttat gttcagcaat
     3361 accaaatgta aagcgaggaa tgacacttat agcagactgt ggcgcaaatg cagattgtaa
     3421 acctaagaat ctagttgaat ttgcagctat gagtaatatt tattcaagaa aagttttagg
     3481 tcttgaaaat cctaaggtag cattagcaaa tgttggacta gaagaaggca aaggcaatga
     3541 tttagtcaaa aggtcatatg aagaaattaa aaaattagat ttaaatttta tagggaatgt
     3601 agaagctaga gaagttataa atgcatacac agatataatc atatgtgatg gatttacagg
     3661 taatatatta cttaaatctg ctgaaggtgt agcactttca gttatgagtc ttataaagga
     3721 aacttttatg gccagtacaa aaagtaaaat aggagcatta cttataaagg atgatttaag
     3781 aaaattaaaa agttttatag attattctga gtatggggga gctccacttt taggtctaaa
     3841 tggaggagtt ataaaagctc atggtagttc tgatgctaaa gctataaaaa atgctataaa
     3901 tcaaggaata aaattttcaa agggaaaagt cgttgaagat attaaccaat ttataagcaa
     3961 atacaatgaa gaaaataaaa ataacgaaga tgaataaact cgttttggag gtttaatata
     4021 taatgaatac aaaggctgga atattaggag taggaagtta tctacctgag caatcatatg
     4081 ataattttca cttcgaaaaa ataatggata catcagatga gtggataagt actagaactg
     4141 gtattaagga aagaagattt gccaaagagt ctgaagcaac ttctgactta gcatctaaag
     4201 ctgccttaaa agctatagaa tgtgcaaagt taaacgtaga ggatatagag ctgattatat
     4261 tagctacaat tactcctgat atgtctttgc cttcaactgc atgtatagta caagatgcaa
     4321 taggagcagt aaatgctact gcatttgata tatcagcagc ttgttctgga tttgtatatg
     4381 gagtaactat tgcaaagcaa tttgtggaaa caggttgcta taaaaatgtg cttgtaatag
     4441 gagcagaaac ttgttctaag tttttaaatt atgatgatag aacaacagca gtattgtttg
     4501 gagatggagc tggagcagct gttattggcc ctgtaaatga gggtggaata ctgtctacac
     4561 atatgggctc tgatggtaaa ggaaaagatt gtttaaaagt acctgctgga ggttcaagac
     4621 ttaaggcaag taaagagact gtagaagcta atctacatac aatagaaatg gcaggtagtg
     4681 atgtgtttaa atttgcagtt agaaagatgg cagaaacttc tttaagagct cttgaaaaag
     4741 caaatctaaa tacaactgat atagactact tagtgccaca tcaagctaat ataagaatca
     4801 tacaagcttc ttctaagaga ttagagcttg atatgaaaaa agtttatgta aatatagaca
     4861 aatatggaaa tatgtctgct gcatctatac cagtagcatt agatgaagct tatagagaag
     4921 gtaaaattaa aaagggtgat aatgtagttt tagttggctt tggtggaggt ctaacttggg
     4981 gggcttcagt tgtcaagtgg actttatagt ataaaatatc taaaatgatt taattaacaa
     5041 aattaaactt atacacaaaa taaagttgat taataataaa aagtttactt ttatgatttc
     5101 aagtgaagga gaattgtatg aataaaattt gcaaaatatt aaatataaaa tacccagtta
     5161 tccaaggggg aatggcatgg gtagctactg catcattagc aagtgctgta tctaatgcag
     5221 gaggacttgg cataatagca gcaggaaacg caccaaaaga agctataaag aaagaaattg
     5281 ttgagtgtaa aaaattaaca gataaacctt ttggagtaaa tgtaatgctt atgtcgccat
     5341 ttgttgatga tataattgat ttgattatag aagaaaaagt tcaagttatt actactggtg
     5401 ctggaaatcc tgcaaagtat atggatagat taaaggaagc tggaacaaag gttattcctg
     5461 tagtacctac aatagctttg gcacaaagaa tggaaaagct aggagctaca gcagtaatag
     5521 cagaaggtac tgaaggtgga ggacatatag gagaacttac tactatggtc ttagttccac
     5581 aagttgctga tgctgtaaac atacctgtaa tagctgctgg aggaattgta gatggtagag
     5641 gaattgcagc atcatttgca ttaggtgcca gtgcagttca agtaggaact agatttattt
     5701 gcagtgaaga gtgttctgtc cattcaaact ataaaaactt agtactaaaa gcaaaagata
     5761 gagatgcaat tgtaacagga agaagtactg gtcatccagt aagaacatta aaaaataaac
     5821 tatcaaaaga atttttaaag atggaacaaa atggagctac tcctgaagaa ctggataaaa
     5881 aaggtacagg agctttaaga tttgcaacag tagatggaga catagaaaaa ggttcattta
     5941 tggcaggtca aagtgctgct atggtaaaag aaataacacc ttgtaaggaa attatagagg
     6001 ctatggtaaa tcaagcaaga gagattatgc cagcaataga actgtaaatc aggaggagca
     6061 agatgggaaa agttgcacta gtattccctg gtcaaggagc tcaatatgta ggtatggcca
     6121 aggatttata tgaaaataat actgttgcta aaagtgtaat agatgaggct agtgatgcct
     6181 taaatatgga tttaaaaaac cttatgttta atggaaatga agaagaactt tctaaaactg
     6241 aaaacactca acctgctatg gttacacata gtgtagctgt attaaaagca gtacaagccc
     6301 aagtagattt aaaatatagt gcatgtttag gtttatcttt aggagagtat agtgcattgg
     6361 ttgcagcaga tgcaattgac tttaaagatg ctgtatgctt ggtcaaaaaa agaggtaagt
     6421 ttatgcagga tacagttcca acaggtgttg gagctatggc agcaatttta ggtctagata
     6481 gaagtatatt agaaaatgta gttaaagata tacaaggagg tattgtggaa gtagcaaatt
     6541 ataattctcc tggtcaaata gttatatctg gtgaaaatga aaagatagaa gaagcaatgg
     6601 tgaaatgcaa agaagcagga gcaaaaagag ctgttaaatt aaatgtaagt ggaccttttc
     6661 actcatcaat gctaagagaa gctggtgtaa aattggccag tgaattagaa aaagttaaca
     6721 taactaaacc taaagttgat gtggtggcaa atgtaaatgc agattactat aaaaacgaag
     6781 gtaaagacct gcttataaaa caagtaagtt catctgttct ttgggaagat tctatagaaa
     6841 gacttttaaa tgatggatat gatactttta tagaaatggg acctggaaaa actttaaaag
     6901 catttattaa gaaaatagct tccaataaaa aagcaaatgt caatatttat aatatagatg
     6961 gtatagattc tcttaatgaa tttgtacaaa attatagaaa tggagagata taatgataaa
     7021 tcttacagga caagtagcag ttgttactgg tggctctagg ggtataggaa aagaaatagc
     7081 aaaaaaacta gcatcttttg gagctgatgt agtaatcaat tatacttcta aagaagatga
     7141 agcactaaaa actaaaaatg aaatagaaag tatgggggta aagtgtacct ctataaaatg
     7201 tgatgtgtct aaatttgatg aagtgaatca aatgatagat tctgttgtaa gcgaatttgg
     7261 aaaaattgat atattggtta ataatgcagg cataactaaa gatggtctgc ttatgagaat
     7321 gaaagaagaa gattttgata gagttataga tataaactta aaaggtgtct ttaattgtac
     7381 aaaagcagtt actaaaccta tgatgaaaaa gaagtatgga agaataataa atatgacttc
     7441 agtagttgga attatgggta atgcagggca aactaattat tgtgcatcaa aagcaggtgt
     7501 aattggattt acaaaagctt ctgcaagaga gttagcatca agaaacataa atataaatgc
     7561 agtagcacct ggatttatag aaacagatat gactaaagta ctaagtgatg atgtaaaaga
     7621 atcaacacta gcaaacatac caaagaaatc ttatggtaaa ccagaagatg tagccaatgc
     7681 cgtagcattt ttagttagtg acatgtcaag ttatataaca ggacaagtaa taaatgtaga
     7741 tggtggaatg gtaatgcaat aataatttaa aaagtagaga aatctattaa aataaaaaat
     7801 tttaggaggt acacaatgtt taataaagtt gtagaaataa taatagagca attaggagta
     7861 gaagataaag aaataactat ggaaacatca ttaatgaagg atttagaagc agattcatta
     7921 gatgcagtag aaataataat ggcacttgaa gatgaatttg gtatagaaat accagataca
     7981 gaagcagaaa acttcaaatc tataggagat atagttaagt atatagaagc taataaatag
     8041 tcgagcttta aaaaagtgga ggtatccgat gaataaaaga gtagtaataa caggacttgg
     8101 atgtgttacg ccgttgggaa caggaaaaga agagttctgg agtaatataa aatctggagt
     8161 atctggtata gataaaataa ctaattttga tgctagcaca tatcaaactc agatagcagg
     8221 agaagttaag aattttcatc cagaagaata tataagtaaa aaagaattga agagattaga
     8281 taaatttgct caatttgcaa tagtatctgc taaattagca gttgaagatg caaatcttga
     8341 tttagataaa gttgatagag aacgttttgg tgttataata ggttctggaa taggtggagt
     8401 tgaagctata gaaactcaac ataaaattct tttggaaaaa ggaaataaaa gagtaagttc
     8461 actttttgta cctatgatga taggaaatat ggcagctggt caagtttcaa tatttttagg
     8521 agcaaaaggg cctaacacta atgtatgtac agcttgtgca tcaggaactc attcaatagg
     8581 tgatgctttt aaggtaattc aaagaggtga tgcagacatc atggtagcag ggggttctga
     8641 agctgctgta acaggtcttg cttttgctgg attctgtaac atgaaggcta tgtcaactag
     8701 aaatgatgac cctaaaactg catcaagacc atttgataaa gacagagatg gttttgtaat
     8761 gggagaagga gcaggtattg ttatcttaga agacttagaa catgctttag caagaggcgc
     8821 taaaatatat gcagaagtag ttggatatgg tttaactgct gatgcatacc atatgacaac
     8881 tccagctgaa aatggagagg gagcagctag gtctatgaat atggctttga aagatggaaa
     8941 tgttccatta gaagaagtag attatataaa tgctcatgga acttcaactt attacaatga
     9001 tttatatgaa actatggcta taaaaactgt atttggagaa aaggcatatg acttatgtgt
     9061 gtcatcaaca aaatctatga ctggtcactt attaggagca tcaggtgcta tagaggctgt
     9121 tgtttgtgct atgagtatag aagatagttt tgtgcctcca actataaaca tacaagaagt
     9181 tggtgaagat ttggatttag attatgttcc aaatcaaggt aaagaaaaaa atattagata
     9241 tgcactatca aattctttag gatttggtgg gcataatgct acaatagtac taaaaaaata
     9301 tgtataaata aatattaaat ataaaactaa aaaacaagca cttgttttaa ctgtataaat
     9361 ttatattagc ttaaatttaa agtctcctaa attattttag gagacttttt tattaagatt
     9421 taagtctagc atatgttgta aatcattttg atatattgta ttattaatta ttaagaggtt
     9481 atttatatat gtaagttttc attctacaat atacaaaagg aggactaaaa ttttgaattt
     9541 tagactacca aagaaaaggt tacccaaaaa gataattttt atatgtataa tacttattag
     9601 tattattttg tttataaagc atgaccaaaa tggattgaaa gaaagagaaa ttataaataa
     9661 gggaaaatat atgtctgaaa gccttgtggc agataaggag tatgatgaag caaaaaaagt
     9721 ggttaagact tctttcgaat ctattccaga aaaaaaatac aataaagtat ataatgaagt
     9781 ttgggatatg ttaaagacta tttcatatgt tccagatgga acgaagatag tcattgattc
     9841 tttagaaaag atgagagaat cagatgtgaa attatctgat gaatgtagat ttaatataga
     9901 aaaaagatta gcatcagtgt atataatgga taataattat tctaaggcag tagatttaac
     9961 tgtaaagtct ataaagttgg ctgagaaatt ggggaataat tatgaaaaag caagactaga
    10021 tgttgatttg gcgagcatat ttttaaagat aggtggctat gaaacaggag agaaattaat
    10081 aaaggattct tttaaaatag atattgatga aggtgaaaaa aatggaatgg ttagattata
    10141 tgctattata aatctagcag aaatatatgt agaagagggc gaatatgata aagctattga
    10201 aataagtagc aggataaaag attataaaaa gtttgtaaat acggataatt acaatgattt
    10261 tgatataatg gcctctataa tgcagtcaaa tgcgtatgtg ggcaaaaata atatacaaaa
    10321 ggcaaagact tttttagaaa aagctgatta tcttataaaa tcagatagaa cagtttatat
    10381 attagataaa gatatgtact attatatggc aatgggaaac ttagaatata aaagcgaaaa
    10441 ttataattct gcaatagata attataaaaa aagtttagag atatctacta aaagaaagtt
    10501 aacccaggag aaaataaaaa atttaaataa gatattaaat atatatgaaa aacaaggaaa
    10561 tatagaatct ttaaatgagt atcaaaagat gttaataaat gaatataaag agaacaaaag
    10621 tataagagat tcagagacta gtttctatat aattgataaa gtatcaaacg aaagtaagct
    10681 atttgaaaaa actaaaaaag aaataaaata ttataaaata ttatttacag taattttaat
    10741 tgctatatta gggtctactt ttttatacaa ccgattgaag tattttaaag cacaaaattt
    10801 acatgatggt cttacaaatg tttataatag aaaaagtttt gatattatgt atgaaaagta
    10861 cagagagaaa gatgataatt ttgcacttgt gatgatagat attgataatt tcaagctcat
    10921 aaatgataat tatggacata aatttggtga cactgttata aagggaataa caaataccat
    10981 ttgtgttatg ttagaacaag atgataaagt ctttaggtac ggaggagaag aattttctgt
    11041 attaataaga aataaatctg gtgatgaagt agcagatata attgacagta taagggttgc
    11101 aattgcaaat aaaaagtgga atgatgatgt gactgtgact ataagtgcag gtgtagctca
    11161 tgtttcagat agcaaagatg tactagaaga agctgataaa aatttatata aagcaaaaca
    11221 acttggtaga aataaagtag tatataagta attatatact atataaataa gaggtataaa
    11281 tatgaaacaa tcaatagcta tagtaactga taaaataact aaactatcat ctttcttaga
    11341 agaaaatata cgtttggttt taggtaatta tattgatata aaccactatt atctggaagt
    11401 tttaaaagag aatgataaaa tacaaggtga ttttgtacta gtaatgaatg atgatagatt
    11461 aaacagtatg agaaagcatt tagtacaaga tagtaagatt atagtgataa ggagaacact
    11521 taaagaaaat gaaatatatg gattattttc aataccaaat ggaactgaag tattggttgt
    11581 aaatgataca aaagaaacta ccttagaaac gataagctta ttttacaaaa taggagtaaa
    11641 aaatttaaag tttactccat atattgaagg aaaaatttat aaaaatatag acatagctat
    11701 aacgcctgga gtaaagggaa aagtaccaag ttatataaat aaagtaatcg accttggaaa
    11761 tagatatatt gatatatcta cctttataga aataataaat ggtcttagaa tagatgttaa
    11821 agaaatacgt aaaaatttaa tggcatattc tgagagttta ataagtttag ataatggagt
    11881 taaagataat tataggcaac tgtttttaaa aatagaagaa caagatgtta tattgaatct
    11941 atcaaaagat ggaataatat ttacttcagc agatggtata ataaacacat ttaataaaga
    12001 agctttaaaa attttaggta taaatgaaaa tccacaaggc aaaaacatag aagatatcat
    12061 ttctgaagat ttaaatattt tacttgaaga caaagaaata attgatgaag tggtatttac
    12121 aaataaaaaa tatataaatg taaataaaaa aagtattttt tctatgggga ataaagctgg
    12181 gatatattat aacttacaag aaataacgta tataaaaaaa ttagaacaaa atcttactaa
    12241 taaattaaga gaacaaggtc agatagcaag atatacattt aaagatataa aaactaaaaa
    12301 taaaaatatg gtaaagtgtg tagaattggc taaaaaaata tctaaatcag aactttctgt
    12361 attaataata ggagaaagtg gtactggtaa agaattatta tcacaatcaa ttcataatgc
    12421 ttccaataga agtaatcaac cgtttatagc agttaattgt gcagcagttc cagatagtct
    12481 attagaaagt caactgtttg gatatgaaaa aggctctttt acaggagctt taagagaagg
    12541 aaaaaagggt ctatttgaac ttgctaataa tggaactata tttctggatg aaattggaga
    12601 tatgcctcca ttgctacaga caaagttact aagggtatta caagaaaagc aggttatgcc
    12661 tataggttcc cataatgtta taaacataga tgttagggtc atagcagcaa caaacaaaaa
    12721 cttactaaag atgattagag aaggaaaatt tagagaggac ctgtattata gactaaatgt
    12781 attgccaata gatgtaccat cattgaggaa tagaaaagaa gatataataa cacttatgaa
    12841 tttctttttg aaaaacaata taagactttc gccagaagtt ataactattt tagaaaaata
    12901 taattggcca ggaaatataa gagagttaca aaatgttgct tcttatgtaa gtctaatgtg
    12961 tgatagtatg gtttttagag atgacttacc accatatata agggtagaat gtgctaacaa
    13021 ttatgaagat ggatttggtt atacaagaaa agaatttgag gaaatactga ggattctata
    13081 tgaaagaaga gagtcatcct ctggaattgg aagagggttt ataatcgaaa agcttataca
    13141 ggaaggtatt gagatttctg aaagcaaagt tagaaaggtg ctatcttatt taagtgaatg
    13201 tggatacata tcatctaaat ctggtagagg aggaagtcaa ataacctcag aggggataaa
    13261 actttataat aatataaatt aagctaagta tttgatattg aaatcacata ttttgaattt
    13321 agatatttaa ataataataa acttttatat ttatagagat taatttatat tatgtcagaa
    13381 agaatattgt aatttataat taaatattaa taggttttta aaataggatt agataggtaa
    13441 taggttaagt tagaataacc cattacctat ttttatttgt aagaaaagtg tacatagaat
    13501 tagaagtaaa aaatacaact aataaaacta ttgaaaatac tagataaata ataaataaaa
    13561 ataattatag attttggcat acatattgct aattatagag ataagacgac aaaataattg
    13621 ggaggtttaa tgatgaaata caaaacaaca gggacttgtg ctacagaaat tgaatttgaa
    13681 gtaaaagaaa ataaagttac aaatgtaaac tttataggtg gatgtgatgg aaatttaaaa
    13741 gggctaaaag tattagttga aggcatgaat atagaagatg taatacaaaa gttaaaagga
    13801 atagagtgta aaacaaagcc aacatcttgc ccagaccaac tgtctctagc attggaaaat
    13861 tatatgaata taaagtaata cataaaacaa tcgattagtt aggagtttaa ttatgaaacc
    13921 aataataggg attttaggaa atttaataat aatggaaaat ggtatgtttc caggtcttga
    13981 aaggtcatat gtaaataatg attatataaa tgcagtgcta aaaggaggag gtagtccagt
    14041 aataattcct gtaaatacag ataaagaagt tataaaaaaa cagattgaaa tggttgatgg
    14101 agtcttaata tctgggggat gggatatcaa tcctcaactg tatggagaag aaacaagaga
    14161 agaaacgaca tttatatatc cagaagtgga tgaatttgat ttaattgcaa taagtatagc
    14221 attagaactt aaaaagccta tattaggtgt atgtagggga ctacaaattt taaatgtaag
    14281 tttaggagga actttatatc aagataataa tcttatagaa ggaagttata taaaacatac
    14341 acaatcttct aaaagacatg tagctactca taaagttgat gttaaagaag gaagtatttt
    14401 agagggaatt ttaggaaaac aattacttac aaatagttat catcatcaaa gtgtcaatca
    14461 gcttggaaaa ggattaaaag ccatagcata ctcaaaagat ggtataatag aagctattga
    14521 gaaagaggat gaaaattttg tagtaggagt acagtggcat ccagaaatga tggtagatta
    14581 ctgtgataaa atggaaaaac tatttaaata ttttataaat atttgttcaa atttataata
    14641 taccttgggg ggattgaaca tgaacgaaaa gaacaaaatg ggattaataa gtattatact
    14701 tttaggtatt aatgcagtag taggagctgg agtttttcta ttaccaggag atgcaatgaa
    14761 atcatttggt gtagcaagta tatttgtata tatatttgat atgttgttag ttttatctat
    14821 ggcattctgt tttgctgagg ttgcaggtaa attcaataaa aatggtgctg cctatgtata
    14881 tacaaaagaa gcttttggag atttttgtgg ttttgaagta ggtcttatga aatgggtaat
    14941 aggatgtatt tcttggggag ctttaatagt aggttttcct acatcattat cagcagtatg
    15001 ggctccagca ggtgaaccac atatacaaaa aattataatt gtagtcatga tagttgggct
    15061 tacaattata aacttattag gtgtatctct atcaaaaatt gtacaaaatg ttataactgt
    15121 aggtaaacta attccattaa tactatttat aggaataggt atatttttta taaaaggagt
    15181 taattttact tcttcaacaa tggtacctcc aggagctgga gcaacagagt ttggagcagc
    15241 agcactttta atgttttatt catttacagg atttgaatct atagcagttg cagcagaaga
    15301 tatggagaat ccacaaaaaa atataccaat agcaattata agtgtaatag taattgcatc
    15361 aataatatat atattaaatc aagttgtatg tgttggtata ctaggagatt cgttatctag
    15421 tacatctaca ccagtggcag atgctgcaag aatatgcttt ggaaatatgg gagctggatt
    15481 ggttaccttt ggaaccttgg tttctgttgg tggaatttgt atgtgtggtg cttttgtaaa
    15541 tccaagaagc tgtgtagctt tagctgatga taaaatgtta ccgagaatat ttgcaagaaa
    15601 agataaaaaa ggtacaccat atgtagcgat aatagctact atgctaatca ctataccaat
    15661 agctctatct ggaagttttg ctgaattagc agcaataagt gcagttgcta gattcataca
    15721 atacatacca acatctttat ccgtattagt gtttagaaag aaaagaccag aattagtagg
    15781 tacttttaaa actccttttg gagcagtgat tcctctaatt gctgtatgtg ttggaacatg
    15841 gttattattc caagcatcta tgcatcaatt aattatggga ttaggagctt tggcaattgg
    15901 agtaccatta tactttataa tgaagtctta taatagaaaa gtctatgatg aaaatttaaa
    15961 gaaaatagtt taattatgtt ataacaattt attgagttaa aattagacat tggcataaaa
    16021 ttaaatacaa ataagtatct acaaggctat attaaaatat tattaatttt ttattttaat
    16081 atagccttat attgttatca ataaatgaaa aatattgtaa caatagaaga acaatagtat
    16141 tatgtttata gacaaaaaac ttattaggag gtaaatttat gccaacaata acattaaaaa
    16201 atggtgtaga cgtacttatt agagaaggtg taagagaaga tgctcaaagc attatagatt
    16261 tttataatga agtgggtgga gaaactcatt ttctgtcatt tggaaaggat gaatataaaa
    16321 tatctttgga ggaacaagaa aatgctatag aatcagcaaa agcatccgat aattctgtta
    16381 aactgattgc atttatagat ggtgaaatag ttgggattgc aacgattgat tctaatcaaa
    16441 aagcaaaagg aaagcatgtt ggagtattag gaattgttgt aaaagaaaaa tattggggaa
    16501 ttggattggg taaaaggctt atgttagacc ttatagaatg gtgtaaatca aatggaataa
    16561 ccaagaagat aactcttgtc actaatgaag aaaattataa tgctatagga ttatacaaaa
    16621 aagttggatt tgaagtagaa agcatactaa agaaagaatg ttattataat ggtgtttata
    16681 ctgatttaat aggaatgtca cttttattgg gaatataatt agtgtacatt ttatctcgaa
    16741 aaaagtatat taagtatggt ataattaaaa aagaatataa cttaatataa ttttggagga
    16801 cgaattatga aaaatctgtg taaaatatca gatgattatt taaacagcaa gcttaagtat
    16861 ttaaagttat tatcaaaaca atatccaagt atatcaaaag caagtacaga aataataaat
    16921 ttagaggcta tattaaatct tccaaaggga acagagcatt ttataacaga tgtacatggt
    16981 gaatatgaac catttgtaca tgtattaaaa aatggttctg gtgtaataaa gagaaaaata
    17041 gaagaattgt tttcaaatac aataagagac agcgaaaaaa aaatgttagc aactcttgta
    17101 tattatcctg aacaaaagtt agacctaata attaagcaag aggaaaatat agatgatttt
    17161 tatagaataa atatttatag acttatagaa ctttgtaaat atgcttctag caaatatact
    17221 aggtctaaag ttagaaaact gttaccagaa aattttaaat acataataga agaacttttg
    17281 catgagcatg ttaaaagtga acataaagaa gagtattata aaagtatagt tgaaacaata
    17341 gttgacatag gaatagctaa ggagtttata attgctattt ctacagttat acaaaaattg
    17401 gttgtagata gacttcatgt aataggagat atttatgata gaggtccaag accagatata
    17461 attgtcgata agcttattga acatcattgt gtagatattc aatggggaaa tcatgatata
    17521 ttgtggatgg gtgcagcctc aggtgaaaaa acttgtatag caaatgcact gaggatatca
    17581 gctagatatg cgaatttaga cattgtagaa gacatatatg gaataaatct attgccttta
    17641 gccacttttg ctatagaaat gtataaagat gacccttgta aagaatttat tccaaaggtt
    17701 aatgaccaaa gtgtaacaac aacagaaaag tctttaatgg caaaaatgca caaagcaata
    17761 agtataattc aatttaagct tgaaggtgag gttatcagac gaagacctga atttgaaatg
    17821 gaacatagac tcttgcttaa tatgataaac tatgatgaag gtacgattaa tttaaaaggt
    17881 aaaacatata aattaaaaga tacttattta cctacaatag acaaaaaaga cccatataaa
    17941 ttaactatgg aagaaagaaa tgttattgat aaattagtat cttcatttag aggtagtgaa
    18001 aaattacaaa aacatgtttc atttttgttt tctaaaggaa gtatatattt aaaggctaac
    18061 tcaaatttac tgattcatgg atgtgtaccg ttaaatgaag atggaagctt tatgtctatg
    18121 aatataatgg gaaaagagta taaaggtaag gctcttatgg ataagatgga gtctctagca
    18181 agagaaggat ttttctttaa agataaggca gaagaaaaac tgtatggcat ggatattatg
    18241 tggtatttat ggactgggaa atgttcatca ttatttggta aagatgatat gactacattt
    18301 gaaagatact ttatagctga aaaagagact cataaagaaa ataagaaccc atattttaaa
    18361 cttagagaaa atgagatggc ttgtaaaaga ttatttgaag aatttgattt agaacttgat
    18421 gaatctcata taataaatgg tcatgttcct gtggagagta aaaatggaga gagcccaata
    18481 aaagctaatg gtaaaattct cgttatagat ggtggttttt ctagggctta ccaaaaaact
    18541 actggtatag ctggatacac tttgatatac aattctagaa ctttgcaatt agtatcgcat
    18601 gaaccattta attctgctga agaagccatt gcaaatgaaa gtgatatctt atcgacaact
    18661 gttgtagtag agcataaggc aaagagaaaa atggtgagag atactgatga aggagtaaag
    18721 atacaagagg aaatagaaga tttaaaacta cttttaatgg cttataagaa agggctaata
    18781 aaagaaatgt agatgttata gaggcacttc atgattgaag tgtctttttg ttaatactta
    18841 ttaaatatat tataaagatt aataatagta gtttatatta tacttactaa gtgaatccaa
    18901 gataaatggg ttcacttttt ttatttataa acattattaa aaaaatatag atgttttggt
    18961 tatattcctt gtcctttata aatagaatta tataaacaaa ggaggaggac aagagatgaa
    19021 taaactttct gatgaaataa taaactctct ttctacaact ataagagaaa aggtagaaaa
    19081 agtctcaaac aataatctga acatagaaga aattaggtta cgctcacaaa aaccattgat
    19141 actaaatgca aactctaaag attattttta caatcaaaaa actatgacac ttgatttaaa
    19201 tcaacaaaac tcatatgtag taacaagaga agatgtagag caaacttttc aaataatatg
    19261 taaatattca atacattcat ttatggatga cataaaaaaa ggattcataa ctttgagagg
    19321 aggtcataga gtagggttag ttggaaaagc aatagtagaa gatggacaag tcaaaaatat
    19381 aaagcatata tcatctttaa atataagggt atcaagggaa attattggat gctcggataa
    19441 gattttaagt catatcataa agggaaaaaa tcagattaat aatactttga taatatcacc
    19501 acctcaatgt ggaaaaacaa ctttaataag agatatagta agaaatttaa gtaatggtaa
    19561 tgaagattat ggatttaaag gtttaaaagt ggctttagta gatgagcgta atgagatagc
    19621 aggagcttat ctaggtgttc cacaaatgga tgtgggaata aggactgata taatagaaac
    19681 ttgcccaaaa gacttgggta ttacaatgct tttaaggtct atgtcaccaa atgttattgt
    19741 gacagatgaa attggaagtg aaaaagaaat aaaggcatta tacacagcat taaatggagg
    19801 tataggatta ataactactg tgcatggaga ttcaatagag gatatacaaa atagaaaaga
    19861 attaaataga cttctggata aagagttatt taaaaaggtt ataattcttt ctgcaaaaag
    19921 aggagcagga acaattgaaa aaatttatga tttagaagaa aagagatggt attttgcaaa
    19981 ttaaaataat tattatagct ttcttgatag gaagtagtta tttgataggt gaacaaatat
    20041 acaaaactta tactcgtaga cataaacaat taaacgattt aataagagtt ttagaaattt
    20101 tgaggatgga tttatctttt ggactttata cattagaaga aatctttaat aggataggtg
    20161 gaaataaaga gttttgtttt tggaaatttt tctatcaaat atcagaagga cttcacaatg
    20221 aacaaagtaa aactttggaa ataataatat cagaaaatat tgatgtctta agtaaagaaa
    20281 cttatctagg caataaagaa attgaggaac tgaaaaatct aatacttact ttagggaaaa
    20341 gcgatataga gtcacaacaa agaatgatag atttatctat agaaaatttg aaaaaacaaa
    20401 cctatgagac aaaggaagat ataaacaaaa aaggcgtatt gtataaaaaa ttagtgacat
    20461 tcataggtat aggtatatgt attatattga tttaatatgg ggaggtttat tatggaaata
    20521 tcattgatat taaaggttgc aggtgtcggt attctaatat cagtattgaa tatgatattg
    20581 gaaaaaacag atagaaaaga ttgggcaggt cttactacat tagcaggtgt aataatcgta
    20641 ttgggtatgg taataacaga aataagtgac ttgtttaata ctgtaagaac aatgtttcaa
    20701 ctttactaaa gaggagggag taatttggaa ataatgcaat taataggaat tgcaatcatt
    20761 tcaactacac tctgtttagt aattaaaaaa gatagaccag agatagcaaa tttcatagct
    20821 ataataactg gagtaataat actattgtca gttatgttta agctaaattt tatagtagat
    20881 agtatacaag atttggctaa caaggctaac atcccgacta tgtatatatc attaataata
    20941 aaattaattg ggatagcata cttaatggaa tttgcgatac aactttgtaa agattgtggg
    21001 gaaggaaaca ttgcttcaaa actagaattt ggaggtaaaa taatagttat gtcaatgtct
    21061 ttccctatac ttttgtccat tgtagaaatg gtggtgaata taattccata gatgtgtatc
    21121 ttggaatgaa tataagtata ttagagagct tttgttgtat tgaggtttat agagtgaaga
    21181 gaggcatggt tgcttatgaa aaaaagattt ttaccaatga ttataggttt tttatttact
    21241 ttcttttttg taaatatctt tgctattatg atatttgcta atgaagtacc aagtagtgaa
    21301 gataaagaat atggtgaaac aaaaaatagc atagataagt atatagatgg tcaattagac
    21361 aagctagata taaatgagat tcaagactat ataaataaag aaatagttat taatgatgtt
    21421 aatttaaaat catttgtaaa agatttaatt agtggagaaa aaaatatctt agatttattc
    21481 aataaagatg gattgaagat attgatgttt gatgaattta aagcaagttt aaaggtagta
    21541 gcagtaattt tggtactggc tctgttatca tctattttaa agagtctgga aaactctttt
    21601 tcatcaggtg cagtaagtca aatagcaaca tatataatat ttatcacaat ggtatcactt
    21661 acacttattg gttttaaaga tgtattacag atttgctatg atgcaataga ccatactgta
    21721 ggtcttatgc aagttataat gccaatatta ataacatttt tacttttaat tggattccca
    21781 ataacgtcga ctactttgaa tcctatcttc ataggaggag taacatttat aaatgtattt
    21841 tttaaaaatt ttctatttgt atctattact gttgcatttg gaattttaat aataaacaat
    21901 ttatcaaaaa atataaggct aaagagattt ttctcctttg taaaacaaat aaattatgtt
    21961 tctattggag ctatgtttac tgtttactta ggtcttgtat ccatacaagg attgtatgta
    22021 accagttttg ataaattcag tgtaaagact gctaaatttg ctataggtaa ttttattcct
    22081 gttgtaggtg gatttgtatc tgactctgtg gatattttat tatcttcatc tcagcttata
    22141 aaaaatatat ttggaggaat agggcttatc ttacttgttg gaatttgctt acttccagta
    22201 ataaaaattt tatcagtaat agtagtgtac aaattagcag caataattgt agagcctgtt
    22261 ggagaggatg gaatatctaa ttttttaaat gaagtggcaa atttaatgat aataatgcta
    22321 gcatcagtaa ttgctataac agtaatgttc tttgtaactg tagctatctt gacctcgata
    22381 agtgtcgtaa gtcaagggta atttatatat cagtgaaaaa taattacatt taatctaatg
    22441 tttttagaga ggaggatgct atgttagagg gcataaaggc atggatagtt agtgttttga
    22501 taggagcatt tatagtaaat atagtagata tgattttacc aagttctaag ataaaacctt
    22561 atgtcaacct agttttaaac tttatgtttg tatttattgt aataactcca gtagtaggat
    22621 ttttttcaaa agatatgagt ttagaagata gaatattaaa atctatggga aactacaaca
    22681 agcagtatgt agatagtaca aatgctttgg caaaagagac tggaaataat agtctatcca
    22741 agggatatga ggatgggtta aaagaagtac ttaaattaaa acttgatgaa tatggttatg
    22801 atttagaaga tatagagctt aatggggcaa atataaataa tataaaaata aaagaaaaga
    22861 ataatagtgc aaaaagtaat aacagttcca atgaagaaaa taaaaataat agtactaaaa
    22921 gagaggaaga aaacataaac tctaatgaca aagaaaattc taaacaagtc ttcaaaaaag
    22981 gtacagagta tgggttgaac ctaaatgaag aaaaattaaa aaacgactta ataaaggtgc
    23041 tggatgtttc tatagaagat atacaaattg ataaatagga gttgggagaa tgttgaaaaa
    23101 cttaaatgac aaagataaga gaaaggtata ttcactaata actatagcag gaatatgtgt
    23161 ggtttcatta gtattattat cttgttttcc tagtagcaaa agcgataaac aagttggaaa
    23221 aacagagact aataaaaatg ctgaaaagca agtcactaaa gaacaagaaa aagatgattt
    23281 agagtcaaaa ttaacagcaa tactatctaa aattgatggt gcaggagacg ttgatgtaat
    23341 ggttacattt gaatcaagtg aagaaataca acctgctttt aattcaaata atacaacaga
    23401 aacaacagag gaaaaagatg cacaaggtgg agaaagaaca gttacgactt ctagtgaaaa
    23461 taaaacaatg ataacttcaa attcaagtga ccctgtagtt ataaaaacta cagagccaaa
    23521 gataaaggga gtgatagtgg tagcaagtgg agcaagtgac ccaagtgtta aagagacact
    23581 ttatagtgca gtacaaacat cattacaagt agctggtcat caagtagaaa tatattctaa
    23641 ataaatacat ataaccgggg ggataaatga atgaagttta attataaggg aagaggattt
    23701 gtaataataa ctttaactgc aatgttagtg gtagtaggaa cagtgaatta tcaactaagt
    23761 aagaaatctt tattagaaac atcaaaagag tttaaggcat atgaagaagc acagttgcaa
    23821 aaaaatactg atgatagtta tagtagtaag acagaggatt ctagtaatgt agataaacaa
    23881 ggtggtaagg aaagtgctga tattgatata gtcgatagca aagctagtaa agtaaaagaa
    23941 aaggcaactg agacaagtaa ggaaataaaa gctcaattat catcagagaa aaatatgaaa
    24001 aaggcatctt atattttaga tatgaaaatg aatagagaaa aacaaagaaa tgagttagtt
    24061 caagatttaa atgaaatgat aaataatcca tctacaacag aagaatctag aaaagaagct
    24121 tctaatatga aattaaatat agttaaaatt caagaaaaag aacttcaaat agaaaacctt
    24181 ttgagtacaa aaggatatga agaagcgtta gtttatataa gcgataacaa agttaatgta
    24241 gttgtaaatg aagctaaatt agaaaagaaa gatgcagcaa aaatatttga tttagttgca
    24301 gaacaagcca atgtgaagta tgaaaatatt aaacttacaa ataataatag taataagtaa
    24361 agtataaacc tcagaattaa ctgaggttat tttcttgcaa agtagacttg tatttggtat
    24421 tattatactt ataaaaatag ttccttaaaa tcaggaggta agttttaata tggaagataa
    24481 taaatttgga caagtaaaaa tatctaatga tgtaatagct acaatagctg gtttagcagc
    24541 attagaagtt gaaggcatag aaactacagc aacattaaca gataagttgt taaaaaataa
    24601 tggagtaaaa atacaaatcg aagaagagga tgttaattta gatgtaatgg ttacgataaa
    24661 gtatggaatg tcaataccag atacagcttt taaagttcaa gaaaatgtaa aaaatactgt
    24721 tgagacaatg acaggactga aggtttctca agtaaatata cacattcaag gaatcagctt
    24781 taagaaggat aaagttgata aagaagaagc aaaagcaact aagaaaaatt aatattagcc
    24841 ctctgaaaag tcagagggtt ttttgctggt aattgatttt atatggataa ttaaacttaa
    24901 aggagaattt tgattgatga aaaaagatag ggcacaaaag agtacaacaa gagaatatat
    24961 aatgaaactt atataccaaa taaatattaa taaagaagat tttgaaactt tagaagataa
    25021 agtagataat tttttaaaag ataattcaga acatattata aatagatata aagaacttgc
    25081 acttcaatac tcaaaaaata caaatttaaa gttagaagat actgaaattg aagatgttat
    25141 agacaaaaaa tatataaata cagtatgcaa agcattgaaa gaaaatcatg acaaaataga
    25201 tgagttaata aataagcatg ctaaaaactg gacagtagat agaatgccaa aggtagatgt
    25261 ttctatactt agattgtctg tgtgtgaaat actttattta gacactccta ataaagtatc
    25321 aataaatgaa gcagtagaac ttgcaaaaat atattgtgat gataagtctc ctaaatttat
    25381 aaatggtata ttgggaagtg ttgttgatga aattgggaaa taatataata attggaattg
    25441 atactagctg ctatactact tctatagcag ctatctcttt agataagaaa gttatattca
    25501 atgaaaaaat aatgttagaa gtaagggata attcaaaagg tttaaggcaa agtgaagctg
    25561 tatttcaaca tatcaataat ttagggattc ttagtgatag gataaaatct tttaaagata
    25621 agtttaatgt agaaggagtt tgctcatcaa aaaaaccgag acctgttgaa aactcatata
    25681 tgcctgtatt caatgtaggt cataattttg gaaaattatt gtctagtata tatggttgta
    25741 gattttatga gactactcat caagagaatc atattgaagc aagtttatta aatagtaaat
    25801 taaaaaataa taataaattt atttctgttc atatgtctgg tggtactaca gagatacttt
    25861 taacaagcaa acaagatagt catcacaatg tatgtgatac taatttaggt aaaattgcca
    25921 aaataagtat taaaaaagat gataaaagta agttatacaa taattttgga tataatatag
    25981 atataattgg aggaagtaaa gatattagct ttggtcaact tatagataga gtaggaatta
    26041 agcttggata taaatttcct tcaggaaagt atttggatga aaatgcttta aattgtaatc
    26101 ttaaaataga aagtggtctg aaaacttctg taagagatgg gtatatgaat ttatcaggat
    26161 tagaaaatca ggtaaataag attataaatg ataatggaga taatactaat caaaaagaat
    26221 atatatctaa attagtatta gattcagtag ttagaaatat gtttaaatct ctagtgtatt
    26281 tatgtgaaac ttataatgta aatgaagtag tatttgctgg tggagtttct gcaagtaagt
    26341 atattcttag agaattaagc atgaagttaa gaaaaaagca tatagaagct tattttacag
    26401 aaccacaata ttcaactgat aatgctgtcg ggtgtgctat aataggattg aataactttt
    26461 taggagaaag agtatgaaac taagagcttt agatataagt gaagcaaact catatattaa
    26521 aagaatttta ataaacgacc caatactttc taatcttaaa gttaaaggag aaatttctaa
    26581 ttttaaggtt catagtagtg ggaatgtata tttatcactt aaagatgaaa catcaaagtt
    26641 aaactgtgta atttttaaaa gtaattttaa tcgcaattta aaattagata atggtgtaaa
    26701 aattatagct aatggatata tatcagtcta tgaaagagat ggtgcgtatc aattatacat
    26761 taatgaaatt gaaatagagg gaataggaaa tttgcacata gagtttaata gattaaagga
    26821 aaaacttaat aaagaaggat tatttgaccc taaatacaag atacctatac ctaaaatgcc
    26881 aaactcaatt ggcgttataa catctccaac aggtgctgtt ataagagata taataaatgt
    26941 aataaaaaga agatatccga aagttaatat aaagctttat ccagtaacgg ttcaaggaga
    27001 taaatcagct gaagaaatct gtgaagcaat tagatttttc aatcatatga agaatgttga
    27061 tacacttata gttggtagag gtgggggttc aatagaagaa ctttggtctt ttaatgatga
    27121 aatggtagca agagaggttt ttaattctca aattccgata atatcagcag ttgggcatga
    27181 aacggatttt actatttgtg actttgtgtc tgatatgaga gccccaacgc catcagcagc
    27241 agctgagatt gctactccat cattggatga tataaactat aaacttggaa acataaaaag
    27301 taggatgagt aagtctctta caaatcaaat agagctagac caatataggt tagaaacagt
    27361 atttaataag ataaataact atttagattc gtacacaata aaagataaag ttatacaatt
    27421 agacaaaata tatgataaaa taatttttgg aatagaaaat aatttaaaat tagaagatga
    27481 aaaattagta aaaattggag cattactcca taatttaagt ccattagcta ctatggatag
    27541 aggttatagt ataactcaaa aaaatggaaa agtaataaat agtataaaag gattaaaaat
    27601 aaaggatagt atagatatag tattaaaaga tggaaattta gaatgtatga tagataaaat
    27661 tgaaaataaa gagggatagt atgaatttaa cctacgaaga agcttataaa agattagaaa
    27721 gtattttaag tgaattagaa tctaaaaatg ctagcttgga tgaatcttta agcctatatg
    27781 aagaaggtat aagcctttat aaacattgta ataagctttt agatgatgct aaattgaaaa
    27841 taagtaaatt taatcaattg ggaatagaag aagactttaa aatagaggag gaataaaatt
    27901 ggaatttaaa caatgtctaa aagaaaaagc aagttttgta gaaaaagtac ttaaagaata
    27961 tatgcctaaa gaagaagggt atcaaaaaac tgttattgaa gctatgaact atagcttaag
    28021 tgctggaggt aaaagattaa gaccaatact tacattggag gcttgtaaaa tagttggagg
    28081 taatgaagat gaagctattc cttttgctat tgcaattgaa atgatacata catattcact
    28141 aatacatgat gatttgccag ctcttgataa tgatgattta agaagaggaa gaccaacaaa
    28201 tcataaagta tatggagaag ctatggggat tttagcagga gatgctttgt taaactatgc
    28261 ttttgaagta atgcttgcag gctcaatcaa taaagaaaat ccagaaaagt atctaaaagc
    28321 tataaatgaa attgccaaag gtgctggtat atatggaatg ataggtggtc aagtagtaga
    28381 tgtagaaagc gaaaataaac aaattgaaaa agaaaaatta gattatatac atatgaataa
    28441 aactgctgca atgatggtag gctgtatgag agcaggagct actataggtg gagcaaattc
    28501 tgaacaaatg gaagaaataa ctaaatatgc aaaaaatatc ggattatcat tccagatagt
    28561 agatgatata ctagacatag taggagatga agctaagtta ggtaaaaagg ttggaagtga
    28621 tatagaaaat cataagtcaa catacccatc tcttttaggt ttagataaat ctaaagaaat
    28681 tgcacataat ttgattgatg aagcaaagaa aagtatagaa aaattatctg atgatgtaga
    28741 ttttttaaag ggattggctg aatatataat agatagagaa tactaaaata tacagctaaa
    28801 atcgaggagg gaaaaatgga ttttttttcg gagattttta acaatggggc tttgggaata
    28861 agtctgattg cttgtttctt agcacaattt ataaaaatat ttactggtaa ggaaaaaaga
    28921 attgagttat caagaatact tatttcagga ggtatgccta gttcccatag ttcttttgtt
    28981 acaagtttag ctacagtggt aggtattgaa aaagggttta attctactga ttttgctata
    29041 attaccgtgc ttgccttaat tataatgtat gatgcagcag gtgttagacg ggctgttggt
    29101 aaacaagcta ctatattaaa tcaaatggtg gcagatatac agcatggaaa gcatatagag
    29161 cagaagaaat taaaagaatt aataggtcat acaccactag aagtttggtt tggagcttta
    29221 cttggaatag taacagcttt aatattgatg taaattgaaa atttacagaa taaaggtgag
    29281 gataatatgt ataaatattt agataaagta aattctccaa aggatataaa aaatatgagt
    29341 atagaagaaa tggatttact tgcaaaggat ataagaaagt tcttagtaaa atctgtttca
    29401 aagacaggag gacacttagc ttctaatcta ggagtggttg agttaacatt agcacttcat
    29461 aaagtatttg atagccctaa agataagatt gtatgggatg tagggcatca atcttatgtt
    29521 cataaaatag ttactggaag aaaagattgt tttgtatctt tgagacagtt taatggacta
    29581 agtggttttc caaaggagaa tgaaagtcct catgatattt ttgatacagg acatagcagt
    29641 acatctattt ctatagctac aggtattgcc tgtgctaggg atataaaaaa agaaaactat
    29701 agtgttatat ctgtgatagg agatggttct ataacaggag gtatggcact tgaagcttta
    29761 aatcaattag gatatataga taccaatatg atagtaatcc ttaatgataa tgagatgtcc
    29821 atagataaaa atgttggagg aatgtctaaa tatctgtcaa gtattataag aaactcaaca
    29881 gttgagaaaa tgacagatga agttgataaa attttaaatg ttacacaaac aggtgaaatt
    29941 ttatctaaga ctgcacatag atttaaagat aaactaatgt atagcttttc tcctcaggac
    30001 tgttcatttt ttgattcatt aggtataaga tattatggac ctatagatgg ccataatact
    30061 aaagaactaa tagatatatt gagaaaagct aaacacaaaa aagggcctgt tcttttacat
    30121 gtaattacga aaaaaggaaa agggtacaga tttgcagaag aacaacctga taaatatcat
    30181 ggagtatcaa agtttgatat aaagacaggt gttacatctg caaaagttaa gtctatgtca
    30241 attagtgttg gagaaaaact tgttgatatg gctaatagta atgaaaatat agtagcaata
    30301 acagctgcaa tgccatctgg tacagggtta aacctgtttg aaagtgcata tccaaaaaga
    30361 tattatgatg ttggtatagc tgaacaacat gcaacaggat ttgcagcagg tcttgcaaaa
    30421 aatggtatga aaccttactt tgctgtgtac tcatcatttt tgcaaagggc atatgaccaa
    30481 gttattcatg atgtatgcat aacaaaaaag ccagttactt ttcttataga tagagctgga
    30541 ttagttggaa atgatggaga aactcatcat ggtatgtttg atttaagtta cttaaattct
    30601 attccaaata ttgtagtaat ggctccaaaa gacacaagag agatggagct tatgatggat
    30661 ttgtcattaa aattagattg tccactagca attagatacc caagaggaag tagttattac
    30721 ctagataagg gagagtatgg agagattgta ctgggtaaat atgaggtatt agatgacggt
    30781 caagatacag tcatactttg tattggaagt atggtaaaac atgctttaga ggctaaagaa
    30841 atattatcaa gagaaggaat aaatccgaca atagttaatg ctagattctt aaagccaatt
    30901 gatgaaggta tgttaaaagc attattaaaa aatcataaaa atgtagttac tatagaggat
    30961 aatattgtaa ctggaggatt tggaagtaga ataaataagt ttattataga taatgaatat
    31021 aacgtaaata tacttaatat agcaatacca gaagaatttg taaaacatgg caatatagat
    31081 gaattatatg actttgtagg attatcgcca aaaagtattg ctgataagat aaggaaatta
    31141 gttattgagt aaaaatctaa tgtagatatt gtaaattaaa atttatataa aatatagaat
    31201 ttatcaatta gaataaatat caagtgtgtt acttcaagct actttagtta tcattaaagt
    31261 agcttttaac agaaaggtaa ttgtatgaaa aaaagaatag atttattatt agtagaacaa
    31321 gggtactttg aaagtagaga aagagccaaa aaagcaataa tggctggatt ggtttttgtt
    31381 gataatcaaa ggtgtgataa ggctggtaca gaagtaaaag aagattgtag tatagaagta
    31441 aaaggaaatc caataccata tgtcagtaga ggtggtttaa agcttgaaaa agcaatgaaa
    31501 aactttgatt tgactattga tggaaaggta tgtatggata taggggcatc cacaggtggt
    31561 tttacagatt gtatgctcaa aaatggagca ataaaagtat tttctataga tgttggttat
    31621 ggtcaacttg catggaaatt gagacaagat gatagggttg tgtgtatgga aagaaccaat
    31681 ataagaaatg tgacaataga agatacaaag cagtttgctg attttgcgtc aattgatgtg
    31741 tcttttatat cgttaaagtt agtacttcca aaggcaaagg agttagtacg tcatgatggg
    31801 gaagttgtcg ctttaattaa acctcaattt gaagcaggaa gagaaaaagt aggtaaaaaa
    31861 ggagtagtta gagaaaaatc tacacatata gaagttataa aaatgatttc tgatttctca
    31921 gtcgaaaatg gatttgagat tttagggtta gatttttctc ctattaaagg tcctgaaggt
    31981 aatatagaat atctaattca tttgagaaat ggaaatgaag gatatgaatt tgatggtgag
    32041 acatacaata acaaaatagt agaagttgtt gaagcttctc ataatttaga caaataggtt
    32101 aaaagggggc ttagatttga tcctcgaatt atatatgaaa aattgtgcct tagttgaaga
    32161 actaaggttg aatattgata aaaacttaaa tatacttaca ggtgaaactg gttcaggaaa
    32221 atctataata atagatgcac ttggactttg tttaggtgat aaatatgata ggtctttttt
    32281 aagaaaagga acagataagg gacttgttga ggctgtattt ttttcagaca atagatatct
    32341 taaaaaaata ttagaagaaa atgatataag tatggaagat gataatttat tggtaataac
    32401 taggttgata tactcagatg gtaagagtac tgctagagta aatggaagga ctgttaaagt
    32461 gtctcttctt aaagaaattg catctacact tatagatata catggtcaac atcaaaatca
    32521 agctttgttt aataaagata ctcatttaaa atttctagac ctttttggag aaaatgaatt
    32581 ggaagagttt aaaattgctt ataaaaatgt gtaccataag tatagtgaag taaaaaaagc
    32641 tttaaattgc ttgacagaaa ataaagatga gatgcaaatt caacgagaaa tagatttatt
    32701 aagatttcaa ataaatgaaa ttgaagctgc aaatctaaat aagaatgaat atgaagattt
    32761 attaaaacaa agagaagttt atagaaatag tgaaaagata tataataatc taaattcgag
    32821 ttatagcaag ttacataatg gtgagtataa tgttattgac ctaataggtc ttgcatctaa
    32881 ggaacttaat gacatatcta aatatgacag tgttttaagt gaatatagtg atactgtaga
    32941 aagaatcatg tatgagttac aagacatatc tggtgagata agaaattaca aagataatat
    33001 agactttgaa ccttatgagc tagagcagat tgagcttaga atcgacgaga ttaataactt
    33061 aagaagaaaa tatggagaca gtatagatga tatatttgag tattatggaa aaattaaaga
    33121 tagattagat gaaatattaa acagagatga aagagttgaa cagttaagaa gcaaactgat
    33181 gaatatagaa gaagatttaa aaataaaagc cagtaaattg actaaagcaa gaatagaagt
    33241 agcaactact ttagaaaaaa tacttcttga tgaattaaaa agcttaaata tgaaaaatgt
    33301 aatgttcaag gttaattttg aaaaaggtcc atttacttta gatggagtag atgatattga
    33361 atttatgata tcttttaact taggagaaga tattaagcct atatataaag ttgcatcagg
    33421 tggtgagatg tccagattta tgttagcgtt taaaaccata ttggctgata tagatgatat
    33481 tgatacatta gtgtttgacg agatagatac tggaataagt gggatagcag cacagatagt
    33541 tggtgagaaa ttgagtgata tagcaaagaa aaaacagata atatgtataa ctcatttacc
    33601 tcaaattgct gcaaatgcag acactcatta ttgtatagag aaagatactt caaataatag
    33661 gacgtttacc aatgttagta agttaaatga atctcaacga aaaaatgaaa ttgccagact
    33721 tatagcaggt aacaatataa cagaaaaaac tattgaacat gcaagtgaaa ttattgagtt
    33781 ggctaaaaaa tgttagtgaa aaacaagcat tttaataggt gtaaagaatt tttatagaaa
    33841 tgctttacat ctatttttta tgatgaaatt attttaaaat atctagtgcc tatattgaag
    33901 aaaagtaggt ttgaaaatgg aatgaacttt gtataaattt gtaactttga aatttctaaa
    33961 tgcttaagta taatattatt tattaaggta gtagtgaggg gaggcataaa atgaataaaa
    34021 aagatactat gcacttaatt tttataattg taatttttat tttattatgg tatattgtgg
    34081 tagcagtaag tgagattaat aaaaatattg aaagttgtag taacaaatta gattatttgt
    34141 cacaagagct tatgagtact aagtcggata ttagtaatac tataaatgaa gaaatgtcaa
    34201 aaagctatat aacaaaaagt attgacttaa aagtgaaaaa aatcgagaaa aaagaatgta
    34261 ttattgatat agatgttcaa ttaagtaaac ttggaaaaaa tggaaaagta ttttttatgt
    34321 ataaagaaga tagtgataaa tggaatgaaa ctgagatgac aaaacatgga gaattatcat
    34381 atgcatgtga aattaagata aatacaggaa gtgaatatga ctataaagta gttacaagtg
    34441 gagctatatc tgaaagttct gatgtgcaaa aattgactaa atctgattat atgcctgaac
    34501 aacccatatt taattctggg ataagagata atagagaata ctttattgaa atagtagaaa
    34561 atagtaatct tttcaatcat gagagtgaaa agtcaaaaaa taaagtttat gataatatta
    34621 gattagaaaa agtagatatt attgtaaatg aaggaaaaaa agacactata tacaaagcca
    34681 aattagctga aggcttagat gatggcaaaa ctaataattc aaatagaaat caagtgaatt
    34741 atgaagtaag ttttcctaaa agggatattg atgacataat ttatattaaa gctaaactta
    34801 catacaataa tggagtagaa tatacaaagg atataactga agatataact atgggattac
    34861 aagatttaga aaaataaata catatttaaa taaatattca aatagatttg ttcaataaaa
    34921 tagagtataa ataaaactgt agtagtttta cttatactct attaaaaaat tataatttta
    34981 attccatata tctagcccct tcaattggat tataagtata aggttctata tcataaaaac
    35041 caaacttttc gtataatcct tgtgcacttt tcatgcttgg aagagtatct aatctcatgt
    35101 gtgtatatcc aaatttttta gcttcttcaa tgatttcttc caatagtatt tttcctattt
    35161 ttaatcctct aaattgattt cttacatata atctttttaa ttcacatatt ttaccttcaa
    35221 gtttttttaa agcaacacaa cctgccaaat tttcatcaac aaatgccaat atcaatgagc
    35281 ctgaaggttt tttatatttt cctggaagtg tttttaattc attattaaag tcttgaaaac
    35341 ataagtctat atttagagaa tttgaatatt cagtaaatag aagttttacg ttttcaaggt
    35401 tttctaaatc tttttcttca acaaattgta aagttatatt ttgagccatt ttatccgcca
    35461 ttatatacac atgtttatat aatgacttca cctccttgat atatgtatat ttttcttaat
    35521 atcaattaga ttttaatacc attacttttt caccaccata gtcaagctct ccattaaact
    35581 caaatccaaa atctttataa agcttaatag ccattttatt gtcttcgaat atactaagat
    35641 atagttcatc acagttatat tcatttttaa gatgatttac aagaaaatct aatgatgctt
    35701 ttccatatcc tttaccttga tattttgaat caatcataaa tctatcaagc caaactctat
    35761 catcttctaa acgaccatac atagcaaatc ctattaaaga attttcatca tatattccaa
    35821 caggtaccca agctgttata aatttagatt cagcaataga aagtgcattg ctttctatat
    35881 aatctaactg acttttatct acagacaaac taactaaatc atgccaatta tttatgtctg
    35941 caattcttac atttaatttc aagatattac cgcccttcaa actttttgtt acaatctaat
    36001 actatcatat tatgtattaa aaatcactta ttaagatttt aaataaactt tttaaatgaa
    36061 taaattctca ggaaaaagga attataaata cataataata cagcatagga gtagatggat
    36121 atatgaacaa aataaataaa aatttcaaaa atatttggat aatattagga gctgtatcga
    36181 tttttaccat ggttataatt tttaataaaa attgtaatgt taataaagag actcaaatag
    36241 aagcttttac aattaagaat catgataaaa aatttgtata tcctatggga aatataattg
    36301 gagtaaaagc aaatacagat ggtgtattgg tcttaggata tgaggaagag gatgtagact
    36361 atattggagg catacaaatc ggtgataata tagtaaaaat agataataaa aggataaaaa
    36421 atagtcaaga tgtatcagag attttgaata agattaaaaa aagtaaagtt gaagtgactt
    36481 ttgagagaaa aaataagtat aaaacagaag taatagagac taaaaaagaa aatggcaagt
    36541 ataaacttgg attatgggta agagataaaa tatctggaat tggaactatg actttttacg
    36601 accctagtat ggaaaagttc aaagcaattg gtcatgccat aaaagattcg gatactaatg
    36661 aactattaaa gataaagcaa ggatatatat ataagccaga acaattaaaa atagtaaaag
    36721 ctagtaatga aaaagtaggt aaaataaaag gagattttaa tgacagcaat ttaatgggta
    36781 atttctcaaa taattcagag ttaggtataa gtggtaatat tacagaaaac cataataaag
    36841 agtttaatgt agcaaataaa gaaaagcaat taatagaagt tggaagaccg caagatgtaa
    36901 aaataggagg tgcagtaatt ctttttgagg ataaaaacaa aaatataaca agctatgata
    36961 taaaaataga aagtatagta tatgataaag gaaattatag agatatggta ataaaagtag
    37021 tagatgacaa gttattggaa tacacaggag gtatcgtaca ggggatgagt ggagctccaa
    37081 taatacaaaa taataaaatt attggtgcaa taactcatgt ttttagagat aatccgaaaa
    37141 aaggatatgg tatttttata gatgaaatga taaaattgta ggtgaggcat taaaaaattt
    37201 tattatttta tcaattatct aggaggaata taattttgga gtgtcgaata tgctttagag
    37261 tagataatta ggaagcaatt gtgtaaaaag tttagttttc tgtaataaga agatgttttt
    37321 taatgggggg atttttagtg gaaaaaatca aaatagtttt agcagatgac aataaggatt
    37381 tttgtcaggt attaaaagag tatttgtcta atgaagatga tatcgatata ttaggcatag
    37441 ctaaggatgg aattgaagca ttagacttag taaaaaagac acaaccggac ttattaatac
    37501 tagatgtaat aatgccacat ctagatggat taggtgtaat tgaaaaattg aatactatgg
    37561 atatacctaa aatgccaaaa ataatagtac tatcagcagt aggtcaagat aagataactc
    37621 aaagcgcaat aaatctagga gcagattact acatagtaaa gccatttgat tttgttgtgt
    37681 ttataaacag aattagagag ctagtttcta atagagttac acaagttgag ccaaaaccta
    37741 gaccagttca agagactcaa atgactagaa gtgattttgt taagaatgta ggaaatatag
    37801 agacagaaat cacaaatata atacatgaaa taggagtacc agctcatata aaaggatatt
    37861 tatatttaag agaagcaata aaaatggtta ttgataatgt tgagctttta ggtgcagtaa
    37921 caaaagagtt atatccaagt atagctaaaa aattcaatac aacaccaagt agagttgaaa
    37981 gagcaataag acatgcaata gaggttgcat ggagtagagg aaaagttgac acaataaatc
    38041 aattatttgg atatacggta cacaatacta aaggaaaacc aactaattca gaatttatag
    38101 caatgattgc tgataaatta agactagaac atagtatggt taaataaaca agacataaaa
    38161 agtaaggctt tttaattaag gcattggcta taaatgcgta ttacaagcag cgaaacggta
    38221 taaccactag ggttataccg tttcgctatt ttaataaata taaaaatttt atttattatt
    38281 tgcttactat atcaatataa taattttatt atactatgga tatagtatgt gtctttacaa
    38341 gttgtaaact gacagtggtt tattttttaa tataaatatt gactttgatg caggtaaact
    38401 ttgtattttt aagcgtatta tggaatatgt taaataaaaa aatgatgaaa tatagtattg
    38461 taaatgccaa agatgcaaaa caaacttaaa acatttattt tattgttaag taatgctata
    38521 atataatgtg attttaataa tgatagtgga ggtttaaata tgagagtcga ggcccctata
    38581 aaagtagatc gaaaaaccaa aaaacttgct aaaagagttg aaagtgggga aatagcagtt
    38641 ataaatcata tagacataga tgaagttgct gcaaactctt tagtagaagc taaaataaaa
    38701 cttgtcataa atgcggctcc ttctataagt ggtaggtatc ccaataaagg tccagggata
    38761 ttaactgaaa ataatatact aataattgat aatgttggag aagagttatt tgaagaatta
    38821 aaagaaggag aaactataga agttgtagat ggaaagatat atagaaaagg aaaattttta
    38881 ggtgcaggag aagttttaga caagtatgag gtttcacacc aaataaaggc tgcctatgag
    38941 aatttagctg tagaattgga tagatttata gataatacta tagattatgc taaaaaagaa
    39001 aaaggattta tacttggaga agtagaaatt ccaaaagtga aaactaatta tgctaataaa
    39061 catgtattga tagttgtaag aggtcaggac tacaaagaag atttaagtac tatgctttct
    39121 tatatagaag aggtaaagcc agtattggtt ggtgttgatg gaggagcaga cgctttaatt
    39181 gagtttggat atactccaga tgtgattgta ggtgatatgg atagtgtaag tgatgaagct
    39241 ttaaaaaagg ctagtgaaat agtagtacat gcttatacag atggaagagc accaggtctt
    39301 aaaagggttg aggaattagg cttagatgca gttgtatttc ctgcaccagg aactagtgaa
    39361 gatatagcta tgcttatcgc ttatgagtat aaggctgagt taatagtagc agttggtaca
    39421 cattcaaata tgattgattt tcttgaaaaa ggaagaaaag gtatggcaag tactttttta
    39481 gttagactaa aaataggttc aaagcttata gatgctaaag gcgttaattt gttatacaga
    39541 agtaaattaa aaataaagta catatgggca ttaatagcaa cagcattatt tcctgtgttg
    39601 gtagttgcat cattgtcacc aggagttcaa cagttcatac aactaatgca attaaagttt
    39661 agagtattat tacagatgta aattttagga ggataaatat gcatattaat atgaaatatt
    39721 atatagtcac tataggggct atatttatag ctttgggtat aggcatgtta gtaggattta
    39781 atttaaataa taatcaacaa ttaagtgaac aacaagcaaa cataataaat gatttggatg
    39841 ataagtttga tatcttaaaa gaaaaaaatg ataaacttga cagtgactta gcaagtgtaa
    39901 atagagatta tgaagaggca gttaatttta taaataaaaa tgtagataag attttagctg
    39961 gttcgctgaa tggtaaaagt ataggaataa tatctgctaa tgaaaatgac gattatacaa
    40021 aaaatataga agacataata aataaatcta atggaagcat tgcatttaat ataatattaa
    40081 aagaaaacat aacaaatcca gaaaagttaa aggaaatgtc aactaaacta ggtacagatg
    40141 taaaaaatgc aaatgatgca gttaattata taattgacac tcttaaaaag gaagatgcaa
    40201 gtgacatttt aacatatctt caagaactag atgtaataaa gtttaatttt attggagata
    40261 catatctaaa atatgattcc gttgtaatag ctggtggaaa tgatgcaaaa gacagtacaa
    40321 aacagtttga aaaaatagaa aaatttgtag tttcaaaact taaatcagag aataaatatt
    40381 tagtagaagt tcaaaataca ggagttaaaa cttcttatgt agaactatat tctaaaaaca
    40441 aagttgcaac tatagacaat attgatgaag gtataggaag tatatctcta gctattcttc
    40501 tacaacaagg taatatagtt ggtaattttg gtagattgga tactgcaact tcattgttgc
    40561 catctattaa atagaaataa ttttaaggag agaaaaaatg gattcttata taagtataat
    40621 aataccagct tataatgaag aaagtagaat aaaatataca ttagaaagca tattggacat
    40681 taagcaaata aatgaaataa tagttgtgga tgatggttca attgacaata ctgctaaggt
    40741 tgtatctgag ctagaaaatg aaaagattaa attttttaag ttagataaaa atagaggtaa
    40801 aggatatgct ttaaattatg gtatcaaatt agcaatgaaa aatgcagata ttataggttt
    40861 tctggatgga gatttaggaa gttctgctaa agaagttgaa aaattaatag tacctatatt
    40921 aaatggtgag gcggatgtta caatagccaa gtttcctcct gcaaagagaa aaggaggttt
    40981 gggatttgta aaaggtttag ctaaaaattc tgtgtttgag atgactggtg ttgagttaga
    41041 tgcaacttta tcaggtcaaa gaatatttaa aagagaagtt ttagaaaagt ttgatgaaat
    41101 accttttgga tatggagtag aagttggaat gaccattgat atattaaagt atggatttaa
    41161 aattaaagaa gtattagtaa atatgactca taatgaaaca ggaagaaatt taaaaggttt
    41221 tattcataga gggaaacagt attatcacat aaaaaaggtt ttaggacaaa aaaagaaaga
    41281 atggaggtaa tatcttagtg aaagattata tactttatac cattttatta ttaactggat
    41341 tatttggaac ctatgctgta attccactat ttaaaaattt attgataaat ggaaatgtac
    41401 ttagaccaaa ctataaaaaa gatatgatac cagtaagtat gggaattgta tttttgccta
    41461 tgattgtaat caatggaata atacttgctt tctttactac agaatttaag gatttagcgt
    41521 atatatttat gtttatgttt ggtatgatat ccatgttttt tgcagggata ctagatgatg
    41581 tcattggaaa tagagatgta agtggtttaa aggggcattt taaaagttta tttaaaggaa
    41641 gtcttacaac aggaggtttt aaagctttat ttggtggatt tgtagggctt atagtttctg
    41701 ttgctatatc aaaagacata attgatataa tagtaaatac actaataata gctctatcaa
    41761 ctaatttaat gaatcttttg gatttaagac caggaagggc aataaaagca tatttattca
    41821 taatggtaat tatattttta acattgacag gatttgttca agttcttcca ttacttatag
    41881 taccaaatgt acttgcatac tttaattatg atttaaaagc aagggctatg atgggagata
    41941 ctggttcaaa cgtccttgga atatctattg gaatgctcat ttcttttggg tatccattta
    42001 acattagatt aggatggtta atatttgtag ttattatgca tatattcact gagaaatact
    42061 cattaactaa gataatagaa aaaaataaac tattaaattt tatagataaa ttgggaaggt
    42121 agtattatga taagcaaaag agtggggatt gttgaatcaa tagtaagtca aactgaaaca
    42181 ctagatgata taagagtaaa tataaatgga gaaatacaaa gggcatataa ttatcccaaa
    42241 atatctggaa ctataaatat aggtgacgaa gtggttttaa atacaacagc agtagaactg
    42301 agcttaggta caggtggata ccattttgta ataactaatt taaataatat agaatctact
    42361 ttaactgaag gtggacatat aatgaaactt agatatacgc ctcttcaaat aaaagtggat
    42421 tctgttgagg aacaagaaag tatttatcat gataaatttg cgaattttaa aggattagaa
    42481 ggtctttctg ttgtagtagg gactcttcat agtatgttaa ctccatttgc agcttcattt
    42541 aaaagaaata atccaaataa aaaattagtg tatataatga ctgatggagc atctcttcca
    42601 atttatttaa gtaaaaatgt ggatacttta aaagaaaaaa aattaataga tagtacaata
    42661 actataggaa atgcgtttgg tggagattat gaatgtataa atatatatac tgcattaatt
    42721 acagcaaaag aaatactaaa agcggatgca gtatttgtca gtatgggacc tggaattgct
    42781 gggacaggaa caaaatatgg atttacaggg attgaacaag gctctattct tgatgctgta
    42841 aaaaaacttg aaggaagagc aattgcaatt ccaagaatta gttttgctga caaacgagaa
    42901 agacatcaag gtatttcaca tcatagtatg actgtattta aagaaattgt aaatgtaaat
    42961 gtagacatac caataactat atatgatgat gaaaaattga actttataaa agagcaaatc
    43021 aatttaaatg gtttagacaa aaagcataat ataatttata tagataataa taaaacgaaa
    43081 gaggatttag actattttaa tctaaaagta aagagtatgg gaagaaatta tgaacaagat
    43141 gaggcatttt tcaaagctgc aagcacagca gcatattact taatggaggt ttaagatggt
    43201 attagaagaa aaaactatta gtagtgatag ggtttataca ggtaaagtaa ttactttaaa
    43261 ggttgatact gttgaaattc cagggcaagg atatcaaaaa agggagttag tagaagttgg
    43321 tggagcagta ggaatagttg caatcacaga tgataataaa gttgtgttag ttaagcaatt
    43381 tagaaaacct atagaaaagc caatctttga aattccagct ggtaaactag aaaaaaatga
    43441 aagtccaaaa gaatgtgctg aaagagaatt aaaagaagaa acagggtata gtgcaaaaaa
    43501 tattaagcta atacataaat ttttcacttc agcaggtttt tctaatgaga taatgtttgt
    43561 atatttagca acaggtctta ctccaggaga gaataattta gatgcagatg agtttttaga
    43621 tgtctatgaa attgagcttg aagaagcata taacatggta ttaaaaaatg acgttgaaga
    43681 tgcaaaaaca tctattggat tattattagt aaaagatatg tttaaaaatt aatatttata
    43741 aaatgaataa ccctttgtcc taaagcatat tattttataa aggacaaggg ggaattaaat
    43801 tgagaagaac atatagaaaa aatgatttta gagaattaaa tagacaaata attattatag
    43861 gattgttgtt tatgatgtct attgttgttg ggtcttacat aaataaaata ttgccaggtt
    43921 ctagtaacaa tatattaaac aatataaatc ctgctgtaga gtattataat ctaaatataa
    43981 gcataaaaga tacagtgata caaaatttaa aatcagatgc aatctttatg ggctcaatag
    44041 ctctattaag tctatttgta gtaacaattc cagcagtgtt ggtagctttt gtattaaaag
    44101 ggatgtcaat tggttataca ataaatagtt gtatattggc attaaaattt aaaagtataa
    44161 aaatgatttt aataatatta tttaaaaatt tgattattat tccatgtgca atcatattag
    44221 cattaattag tttaagttat tttaaagaaa tggtttatga atttaaaaag aaaaatagaa
    44281 aaaatatgca atttttaatt aagaggtata tactaaatat tataattata atagcactat
    44341 cattggggtt gcaattaata ttaaatactg caagtattgg tattattaag ttcctagcta
    44401 aataaaggag tttataatgg atattataga gggatatata gattacataa aagataaaaa
    44461 aaaattatca gagaatacag tggcatcata ctttatggat ataaaaaaat atatggaata
    44521 tataaatcaa aaaagcatta aactagtaga tatagtagaa aatgatatta taagttactt
    44581 aataaagttg gaaaaagata atgtatcaat agctacaata gcgaggatga tatcctctat
    44641 aaaatctttt catgattatt tatttttaaa tcatatatgt acaaataatc ctgcaaagga
    44701 tataaaaaaa cctaaaataa aaaaagaaaa tataaatata cttacagaag aagaaattga
    44761 aagactatta aattttccaa agttaacaac acctaaattg attagggaca aggctatttt
    44821 tgaagtatta tatggtactg gtataaaagt atctgaatta gtagagatga atatagagga
    44881 tattgaccta gatattgatt atatatattg taattcatgt tgtaaaaatc aaagagtgat
    44941 accactttgt gatattacaa aactttactt agaaaagtat ctaaaagaag caagacctaa
    45001 aatggctgta gaaggtgaaa aatcattatt cgtaagttca ttaggtcaaa ggtttacacg
    45061 acaaggtctt tggaaagtca taaaaaaata ttctaatcta gccaatatag ataaaaatat
    45121 aaatcctaca atgcttagac attcatttgc tattcatttg ttaaatgaag gtgctaacat
    45181 tgctgttgta agtaaaattt taggaaatgt taatttatct agtttgcaag tatatttaaa
    45241 tcatatagat aaaaacgtga gaagagaaat aaaggagaaa catcctagaa atgatgttga
    45301 tgtagaatta caaaaagcag aagctaaata gactatatag caaattttat aatttagaag
    45361 gagcatattt atatgagcag agtaatttgg attgtaattg atagtgttgg gataggagca
    45421 ttgcctgatg ctgaaaaatt tggtgatagt aaggatgtaa gtacccttgg aaatatattt
    45481 aaggaatatc cagatattca gattcctaat atgagaaatt taggtatagg aaacatagat
    45541 ggtatagatt tttttgaaag tataaaagaa ccaataggtt gttttggaaa gtgtaaggaa
    45601 atgtctcagg gaaaagatac tactacaggt cattgggaaa tgactggtat aatagttgac
    45661 aaaccattta agacatttga acatggattc tctaaggaga taatagaaga gtttgagaaa
    45721 aaaactggaa gaaaagtagt aggaaataaa ccagcttcag gaactgtaat aatagatgag
    45781 tatggagaac atcaaataaa aactggagat gtaatagtat atacatcagc tgatagtgta
    45841 tttcagattg ctgctaatga agaagtaatt cccctagaag aactctataa tatgtgcaaa
    45901 atcgctaggg aaataatgat gggggataat gcagtagcta gagtaattgc tagaccattc
    45961 ataggtaaga aaaaaggtga gtttgtgagg acttcaaata gaagagatta ttcactagac
    46021 ccatttgaac caacagttct tgataatata aaagaatcag gacttgatgt tttagcagtt
    46081 ggaaaaatag aagatatttt taatggaaaa ggaattacag atgctatcca cactaaaagc
    46141 aatatggatg gagtggatga aactttaaat tatatgaaac aagataataa aggtctaata
    46201 tattcaaatc tcgttgattt tgattctaaa tatggtcata ggagagaccc agaaggatat
    46261 aagaaagctc ttgaagagtt tgatagtaga cttcctgaaa taatggctaa tatgagagaa
    46321 gatgatattt taataatcaa tgcagaccat ggaaatgacc caacatataa aggtacagac
    46381 catacaagag aatatatacc tgttatgatt tatggcaata agattaaaaa aggttttaat
    46441 ttaggagtaa aagatacttt tgctgatatt ggagcaactg ttgcagatat actaaatgta
    46501 aagttgccta aacatgggtc aagctttaaa gaagatttat tttaaactat aaaatgataa
    46561 aaacttagct taataggatg gagagattgg aatgtttgaa aagatagcac aaagtagtaa
    46621 atttataaat tctaaaagtg atataaaacc taagattggt ttgatattag gttctggact
    46681 aggtgattta gcaaatgata tagaagaccc tgtcgcaatt aaatatagtg aaattccaaa
    46741 cttccctgta tcaacagtag caggacatgc tggacaactt gtaattggaa aacttgaagg
    46801 aaaagaagtt attgctatgc aaggtagatt tcattactat gaaggatatt ctcagaaaga
    46861 agctactttt ccagtaaggg ttatgaaaga attaggagtc gagattctta tagtaactaa
    46921 cgctgctgga ggcgtaaata aagaatttaa agcaggagat ttgatgctta taagagacca
    46981 tataaacttt agtggaagta atccattagt aggaaaaaat gatgacagat ttggtgctag
    47041 atttccagat atgtcagatg catattcttc taaatatgta gatgtagtta aaaactgcgc
    47101 taaagaatgc aatatagatg tcaaagaagg cgtttatatg ttttttagtg gtccaaacta
    47161 tgaaacacca gcagaagtga gaatggcaca aattttggga gctgatgctg taggtatgtc
    47221 tactgttcca gaagtcatag tagcttctca ctctaatata ggagttattg gtatatcttg
    47281 tataacaaat atggctgctg gcatcttaaa tcaaccacta agtcatgagg aagtaataga
    47341 aactactcaa aaagttaaat cagaatttat gaatttagtt aaaacaacgg ttaaaaattt
    47401 ataagttaaa gcttattata gattttttga ggaagtttat aataaaatag ttaatgttta
    47461 atttgaaaag aattaataaa aagctgtctt gatatagaaa gttatttcta ataaaggaca
    47521 gtttttttat aatactacaa catttgaaag tgttttaaag tttaatattt atttaaatta
    47581 ttataggtta gaatggagat ttcaattatg agaatgtatg atattataaa aaaaaagaga
    47641 gataacctag agcttagtaa aaaagagata aactacttta tagaaaacta ttcaaaggga
    47701 gatatacctg actatcaagc ttctgcacta ttaatgtcga tttatttaaa taagatgaat
    47761 aaacaggaaa ctgtacatct tacagaagca atgatgaact ctggagacat gataaaccta
    47821 tctgagatag atggaataaa ggtagataag catagtactg gtggtgttgg agataagact
    47881 accatagctc ttataccttt ggttgcttca tgtggagcac cagttgcaaa gatgtcaggt
    47941 agaggtcttg gtcatactgg tggtactatt gataaattgg agtctatacc aggtttttcg
    48001 acagaaatgg agataagtaa atttattgat tctgtaaata agtctaaaat agcagtttgt
    48061 ggtcaaagtg caaaggtagc aattgcagat aagaaaatat atgctcttag agatgttaca
    48121 gcaactgttg ataatatatc attgatatca tcaagtgtaa tgtctaagaa gttagcttca
    48181 ggagctgatg caattgtgct agatgtaaaa actggtgatg gagcatttat gaagacatta
    48241 gaagagtctt ttgagttagc taaatctatg gttgatatag gtactggtat gaatagagat
    48301 acaataggga ttgtaacaga tatggatgaa cctttaggtt ttgcagtggg aaactcttta
    48361 gaagttatag aagcaattga gacattaaaa ggaaatggac ctaaagacct tgttgagcta
    48421 tgcgaagtat taggagcata tatgttggtt ttatcaaagg tagcacatga ttttgaagat
    48481 ggtgttgaaa aaataagaga atcaataaaa tctggtactg ctatacaaaa actcaaagag
    48541 tttatagaga atcaaggtgg taataaagat gtagtagatg attattcttt attcaaaaaa
    48601 tcaaagtata aatatccagt taaatcaact aaatcaggtt atataagcaa aattaaagct
    48661 gaagatgttg gtgtatcggc tatgattctt ggagcaggta gagaaactaa agatgatatt
    48721 ttagatttat cagcagggat aattttagaa aagaaagtag gagattatgt aaatgaaggt
    48781 gaagttttag cttacatgta ttataatgat gaacaaaagc tttcattggc aaagggaaga
    48841 tttattgatt cctactctat tgtagattca aaaatagaaa aaaataaatt aatatatggt
    48901 attgtaacta aagaagagat taaaaagttt tagaaaattt tagaaaagtg attataaaag
    48961 gcctaacatg tcaaaaataa tgttaggttt tttatttttt gatagactat aaattatacc
    49021 aaaaatatat attttaaaac attttttaca atataaataa gtttattcta caagatgatt
    49081 gaaaattaaa gtatctagtt ttataataga aaatggataa atatacataa ggagtagatt
    49141 atattatgaa ctttaaagaa aatagattga aaatagcagt attaataatt gttattttga
    49201 ttatcttagc aggtatattt gtgtttatac aaataggtcc ttatgataaa aataacaaaa
    49261 aagatgtaat tatcgacgtt ccaagtggag catctgttgg aaaaatatct gacatattat
    49321 atgaaaataa attaataaag aatgaactat tatttaaact tttggtaaag gttagcaata
    49381 aagctccgtc tataaaatca ggaacatact tacttaatca atcgtattca aacaatgata
    49441 taataagtct tttagtttca ggtaaaatat atcaggatgg tataaaagtg actattccag
    49501 agggagctac atctaaagaa attatagcaa tgttagtaag taaaaattta ggagataaag
    49561 ctacttttga aaatttaata aaaaaacctc aagaatttta tgataaattt ccatatttga
    49621 aggaagatgg aataacctct ttagaagggt tcttgtatcc agaaacttat tattttaatt
    49681 caaagaaaca atcagaagaa gatattttaa gcgaaatgtt aaaagtattt gacagtaaat
    49741 acatggacaa gtttaaaaag aaacaaaaag aattaaatat gactcttcaa gaagtaatgg
    49801 aaatggcatc aataattgag aaagaagctg ttcttgacaa agacagacct ataatagcaa
    49861 gtgtgtttta taatagactt aaggtaggta tgcctcttca atcagatgct acaatacaat
    49921 atatatttga agagagaaag aaaattgtaa catatgatga tttaaaaatt gattcaccat
    49981 ataatagtta taaaaataag ggattacctc ctacaccaat atcaaatcct ggtataaaat
    50041 ctattgaagc agcattatat ccagagaaaa cagactattt atattttgta gctaagattg
    50101 atggtggaaa taattatagt acaaactatc aagaccattt aaaatacgtt aaagaatata
    50161 aagaagcaag ggataaacaa tcaaaagata caaaagcaac aaataaagaa aatacaaaaa
    50221 aataaaccat gattataaaa agtctaacag ggtaagaata atgttgggct tttttattga
    50281 aaattgttat tcttagattt ataataaaga aagtgtaaat atatataaca atatgaattt
    50341 aaaaaataat aaattaatat aaaacttgaa tatttattat tttagggagt gtttttatga
    50401 gtaatatagt taatgatttg gttgaagatt atataagagt gacattaaaa gaaaaagaag
    50461 gatttttaaa agatttagaa atttatgcag aggaaaatag tgttcctata atacataaag
    50521 aagtttcaga tttattaaaa gtcttgttaa aggttcaaaa accaaagaga attttagaag
    50581 taggatgtgc aattggttat tcttctattt tttttgcaac tattatagga aaagatgcag
    50641 atataataac tgttgagaga aatgaaaaaa tgatagaaaa agcaaaagaa aacataaaat
    50701 tagctggatt tgacaataac ataactatac tagaaggaga tgcagaagaa aaacttagtc
    50761 aagtccaagg tgaatttgac cttatattta tagatgcagc caaaggtcaa tataagctat
    50821 tttttgattt agttatagat aaattaaaag atgggggact tttagtatct gataatattt
    50881 tgtataaagg tatggttgct catgatgatt ttgttgtaag aagaaaaaag actatagtta
    50941 aaagaatgag aaattattta gattatatat gtaattgtga ctatttaagc acatcattgg
    51001 taccaatagg tgatggtgta gcactaagtt ataaagaaag taaaagaggt gatgtagatg
    51061 gaattaaata aagtagaact tttagctcca gctggtgatt tggagagatt gaaaatagct
    51121 ataacatatg gagcagatgc cgtttatata ggtggagaaa tttttggaat gaggtctgca
    51181 gcgaagaatt ttagtaagga agatatggca gagggagtag cttttgcaca tgaaagaggc
    51241 aagaaagtat tcgtaacagt aaatataata cctcataatg aagattttct acaattagaa
    51301 gactatttgt tggaactaga agagatagga atagatgcag ttataatagc tgacccagga
    51361 gtgcttagtg tcattaaaaa agtaattccg aatatggaaa tacatttaag tacacaagca
    51421 aatacaacca attatctaag tgcaaacttc tggtacgagc atggaataaa aagagttgta
    51481 gtggcaagag aattatcttt tgatgaaata tctgagataa gagcaaaaac acctttagat
    51541 atggatatag aagcgtttat gcatggtgct atgtgtattt cttattctgg cagatgttta
    51601 ataagcaact atatgactgg aagagatgca aacaaaggtt cttgtgctca atcttgtaga
    51661 tggcaatatc atttggtgga agaaaaaaga cctggtgagt attttccaat atacgaagat
    51721 gaaaggggta cattcttctt taactcaaaa gatttatgta tgatagagta tataccagag
    51781 ttaatcaaat ctggtataac aagtttaaaa atagaaggta gaatgaagac tgcatactat
    51841 gtagctactg ttgtaagagc atacagaatg gcaatagatg aattttacag agacccagaa
    51901 aactggaagt tcaatccaat gtggatggaa gaactaaaaa aaggtagtca tagacatttt
    51961 acatcaggtt tttacttgaa taaaccaaca acagaagacc aaaattatca atcagcatca
    52021 tatgttagaa actatgattt tataggtata gttagagaaa cagaagatga agatggtctt
    52081 attgttgttg agcaaagaaa taaaatgtgt gttggtgatg aaatagaagt tatgggacca
    52141 tataaagaaa ctatgtttac taaaattgaa gaaatgtaca atgaagaagg agaagctata
    52201 gaatcagctc ctcatcctag acaaatagtg aagttaaagc tatcagtaaa agttggtaaa
    52261 gattatatgc ttagaaaagt tatagaagaa aaggttgaag aataataaaa aattgataaa
    52321 aatagtatta gccccttaga aattgtcaat aatgaaatta tctaaggggt gatttcatgt
    52381 caaaaaagaa gactccattt ttgaaaaaag tgggaaagag aagctggtgt atatttacta
    52441 taattttaat aatttatagt gttttaattt ataggctagt tgatatacaa gtattaaaag
    52501 gtgataaata caaacaaagt gttgaatcac aaagcgttga gaaagtcgag ttaaatagtg
    52561 gaagaggaat aatctatgat agaaacaaca agaagcttac agatacaagt aaatctcagg
    52621 tattaattgt tgaaaaagaa aaattaaata ataactataa aattttggag cttattaaga
    52681 aagctactaa gatgaatgat ttagatatat ataaagctgt acaagagcaa ttgacacgcc
    52741 ctataataca gattcagact aaaaatatcg ataagagtat gaaaaaagaa cttgagaaaa
    52801 atggaataat ggtagaagaa aaaactatga ggtacgcaaa agatggttta ttatcacata
    52861 caataggata tataaaagaa gatgataaat caggtcaatc aggaattgaa aagagtatgg
    52921 atagtgtact tagaaattca aatgaaaagt atataagtgc ttttaaagct ggagatgcag
    52981 gaaatgaaaa atctctaaac atactaaagg gaagtgtaaa aactgttgat aataaggata
    53041 aagatagaca tctaaaaact actattgatt ataacattca aaaaaaatta gaacaaatac
    53101 taaataaaga agaaaatcct acagcagcaa taatctcgga agcatctaca ggtgaaattc
    53161 ttgcaatgtg ctctagacct aattttgacc aaaatgacat atcaaaaagt ttaaaaggaa
    53221 aaaatggaga atttgaaaat agagttataa aagcgacata tccaccaggg tctgtattta
    53281 agatggtagt tttattttct gcacttgaaa atggtgttat agatgagaac tatacttaca
    53341 actgtacagg taagactaaa gttgggaata ctaatgaaat actgcgatgt aataaaagag
    53401 atggtcatgg tttccaaaac ttaagacaag ctttttctaa ttcatgcaat cctgcatttt
    53461 tagatatagc tatgaaactt ggaaaagaaa agatactcaa atctgctgaa aaattacatt
    53521 tatttgaaaa ggttgatatt ggtcttgatg aagaaaaaat tagagaagct ccaaaaaaca
    53581 tatcaataag aaatttagct ataggtcaag aaaatataga atttacacca cttcaaataa
    53641 atcaaatgac tcaaataata gctaataatg gaacattcaa accactatac ttatataaaa
    53701 gtcttgtgga caataatatg aatacaataa aaacttataa gtcttcaaaa aaagaagaac
    53761 ttatttctcc ttatgtgtgt acacaagtta aagaatatat gaagtctgta tctaggattg
    53821 gtacagctaa agacctaaaa gatattgaag gtggttgtgg agttaaaact ggtacagctc
    53881 aaagtagttt aaataaaaaa gctatagacc atggatggat aacaggattt tatcctgaag
    53941 aaagacctaa gtatgtaata actgtgttag tagaaggaac tcaaaaagga aataaatctg
    54001 caacacctat atttaaggaa atatgtgaaa gtataaaata aattatgtac tataaaattt
    54061 aaggatatat cgtaactttg attttagata gaaaattttg tctaatgatg tacgatatat
    54121 ccttttaaaa tatagtatta actttcttga acaagctctt tttctagttt tttaagagct
    54181 tttttttcga ttctagatac atatgacctt gatatatcga gtttttgtgc aatttctctt
    54241 tgtgtcttat agccataagg agttaaccca tatcttaact ggacaatttc tctttctcta
    54301 ggagtaagaa ttttgtttag ttgttcgtaa agttttccaa cttgaacttt tagttcaact
    54361 tcatcaagaa catagttagc ctctgtacca agtatatcta aaagacttat ttcattacac
    54421 actactcttt atatgttatt ggtattactg gtcttcatgt aatttcaaat ggaccattaa
    54481 ttcaaaagaa tctagttttt gttctttaga cttataataa actgctttat caattacaga
    54541 ttttaggagt ttattttttc caagggaatc tgaatcatgg taaagtgata acacagtttt
    54601 aatatcttct attattttag aattgtcttt tttaacagta cttaatctat tttttactgt
    54661 cttaattgaa tctttaattt cttcagttct tgaacttata ttgttggacc tttcaataaa
    54721 tgtatcttca tcatatattc ctcgttctag taaatcatat aacttatttt tttgcttgcc
    54781 taattctttt aattcagttt ctaagccctt taagactcgt ttttgaaatt ctatattact
    54841 atctttatta tttttatcta agtctatatc agatgctttt aattgatatt tttttacagt
    54901 gtcttctaaa gattttaaaa tggcagcttc aataaattca aatcgagaac ttttattgca
    54961 accaggatga taacaaatta tatagtcgtg gtctgcatat ggtctatata ctaatggtct
    55021 accacaattt ttgcaaatca ctattcctgc aagtggattt tttatttgat taccatagct
    55081 gacatgacca ttttttttga gtatattttg agcttttttc catgttattt cacttattaa
    55141 aggttcatgc tttccttttg cctcaatcca ttcatcttta ggtcttagtt taactgtttt
    55201 tattttattt ggatttttag attttttgta gtcctttttt tgccactgaa tgtagccaca
    55261 atatacctta ttttttatta tatttgtaat tgcagaattg taccaattag cacctgtagc
    55321 agttttaatg cctaagttat ttaggtattt tgatatttta ctacaaccca tatcttcatt
    55381 aatatataag tcaaagatag tcctaactac atcagcatcc ttatttggta caagtattct
    55441 ttcttttcta ccagttgtta cagcatcata tccaaatgga gcagaggttc ctatgaaatt
    55501 cccttcttca actgatttta ttctacctct ttgcatcctt cttgatataa gttttaattc
    55561 ttttcttgcc ataaatgctt caaattctga atactcctca tcaaattcat ctgttaggtc
    55621 gtatgttttt ctaggtgtga taattttggt cttactttct ttgaatgttt ctaatatgat
    55681 accttggtct ttcatgtctc cacgaccaag tctgtctaag tccattacta agactgcatc
    55741 atatttattt tcttcaactt cttttagaag ttcaagcatt ttgggtctat gtattatact
    55801 ttcaccagaa actaattctt cttttatctc aattacatct aagttttgtt cttttgctag
    55861 ttttagtagg gttgatttat gtctacttaa agtttcaaat tcgccttgtt tttctgcctc
    55921 ttcatcagca cgggattttc ttaagtatat ggcaacactc ataataagta atcacactcc
    55981 ttatgcacta ttatatagta aatcaataga gtttaaaata tagaaaataa gcatgtcaaa
    56041 agttaattta actttcaaca tacttatttt ctataatatt aatgccttat aatcctttaa
    56101 tatcatttag attagttttt gcatattcat tatccgggtt aagttcaagc gccttattgt
    56161 aagattctct agctttatta aattctgatt tactcataaa aataacacct cttaaattgt
    56221 aagcatcact agcgtctatt ttataagctt cattattttt atctagatat tctaagtatt
    56281 tatcaattga ctttaaagcc tcatcatatc gttgtaactc tatataaata tatgattgtg
    56341 tataatatgc aagtggattt ttatcatcaa tttctatagc ttttttgata tcaatagcag
    56401 ctttattata atccttatct tcatcttttt ctgtttctga cctagcgatt ttgacataat
    56461 tagagctaag agctctttta aagtctttat gccataataa tttatattca ttatcttcga
    56521 ttataaaata tctatcaaaa tttgcagtat cttctttatt ttcagtgata tactttatag
    56581 tatcagtttc ttttatttta acaagatttg tgtactcttt attagaatat ttgtatttct
    56641 ctactttatt ttcttcacca agtttataat cttttagggt ttgaagttta aataaagtat
    56701 cttgatattc ttcaaatttt tctagactga cattttcctt actgtcttta cttaataaat
    56761 tatatgctac ttcgtagtct ttgattttta tactatcaag gtacttttta gctacttctg
    56821 tagcactttc ttttggagca catccaacca ttgatatcat aattaaaaat gatactaata
    56881 ttgctgttat ttttttactg attatcataa tataatcccc cttatattgt ctctcattaa
    56941 ttttatttta tcaccatatt ctaccaaagt cattaattta agagtgatat ttttcattaa
    57001 tattatatta agtaataata accttttaaa tttatggtaa cattttcctg aaaagtatta
    57061 tataatttag gtgaaaatat ttaagagaag tagatattat gaaaattaaa aaattattaa
    57121 caatagggtt atctttatct atatttattg ctagttgccc tatatctgca aatgctcttg
    57181 ataaaattga accaattaaa ggtgcagata aatacgaaac tgctggtata attgcagaca
    57241 aataaaacta tactacagct atattaatta atgcagacag tacaatgtct gatggattaa
    57301 gtgcaagtgg acttgcagga gctataaacg ctccaattct tttgactaag aaaaataaca
    57361 taccaaatgc tacacttaaa agattagaaa aggcaaaaaa agtatatata attggaggag
    57421 aaaactctat tgataaatat actgaaactg ttctaaaagg caaaggaata gagattaaaa
    57481 gacttcaagg tagtgataga attaagacaa gctataatgt cgcaaaagaa ataaactcaa
    57541 ttaacaaagt aaataaagtc atattgacta atgcttttaa aggagagcct gatgcaatga
    57601 gtgctgcacc tgtagcagtt agagataaag cagctatagt attaactgat ggaaaaagtg
    57661 tccatttaat acaactggtg tagaaagtta tgtaataggt ggtacttcat caatgaatga
    57721 caaaatagtt aaggatacta attcaactag attaggtggg acagatagat atgatactaa
    57781 caaaaagata ataaataaat tttatagtgg agtaaaagaa ttttatatag caagtggtac
    57841 tgatttagtg tatgctcttg ttggttctaa cattgctaaa aataatgcta ttgtgttagt
    57901 tggtaatgat agtaataaaa gtgtattgaa aagtacaact aaattaactg ctataggaaa
    57961 tttgagtgat agtatattac agcagtgttt aaatgtaaca aaaaatatag gcgattctaa
    58021 tactggaaga gaatatacag tagaagatgc tgctaatgta gtgaaaaaac tattaggtgt
    58081 gaagaaattt tattacgaac ctaattattc agaagatgaa gatttaactt acaatctata
    58141 gagtggagtt catgtacaaa agattatcct cccaacgatt cgaagggagg tgagttcatg
    58201 gataactttt tgtttaatgt tttagctagt ttaacagcta gtgtggtagt ttacttaatc
    58261 agtaaactat tcaaaaaagc aaaaagccac tctcgcacaa agagtgactt acgagttgaa
    58321 tttaaattta tatttaaatc caaaaaataa actctgttta tgatgaactc cactctaacg
    58381 caaaatagat tgtagttctt cttgctttta ttataccaca aaacattata aataaaactg
    58441 ttgtttataa aaaaatgcaa agtaaaattt tttataaaca atgataaaaa ttttattata
    58501 gagctattaa cttatgaaat atctgtaaat gagcagatat tttataagtt aatatttatt
    58561 gtttacataa aatatttggt aaaaataagt aagttttata tgttataata gttgtagcaa
    58621 gaataataat ctaaagtggc aagcatgtca gctggtgtta tatcagttta tatttatgat
    58681 aaaataaaaa tctcccaaac gccaataaga atgatttaaa aaaataatat tttcacttaa
    58741 aaataatcac tctttatagg agtaaattat tttcttgatt ttattatact acaaattggt
    58801 acagatattt aaaaataata tattcataat attataaata gttttgcagt gagcgatatt
    58861 tttgataaaa tagggcttaa cagttgaaac ataaggcatt gagggtatat gataaatatt
    58921 atcatttgca ctactcgtgg ttcactgcaa atttgagaga attgtataga tgtaagtgtt
    58981 ggaaatactc aatttatttt ggggttttat attaactaag tggtatgtaa aggcaataaa
    59041 acctgctatt cttggtagaa attcatttgg ttttatatta actaagtggt atgtaaagtt
    59101 tgacgtgtta agtgatatag atagaagaat atgtgagttt tatattaact aagtggtatg
    59161 taaagtttgt ttctctgtaa catgattatc attcataatt aaagttttat attaactaag
    59221 tggtatgtaa agagaggata aattgtttac atagtaccag ttgggtcttg ttttatatta
    59281 actaagtggt atgtaaagtt gcacttgaat agttctgaag aacttaagaa ctatggtttt
    59341 atattaacta agtggtatgt aaagagcttt acaatatcaa agaaaaattg aaacatctat
    59401 gttttatatt aactaagtgg tatgtaaagg gaaaattatg ggaaaagttt acagatatca
    59461 tgagagtttt atattaacta agtggtatgt aaagtaaaaa taaacctcct aaacaaatat
    59521 ttcacgcata gttttatatt aactaagtgg tatgtaaagc ttgtaatcgc tgaataaaga
    59581 tttctagctt cgttattgtt ttatattaac taagtggtat gtaaaggact ctgccatccc
    59641 tgtaacgaac agccttacag caagttttat attaactaag tggtatgtaa agacttatct
    59701 gtaaccctta tatgttcaaa ttcatagccg gttttatatt aactaagtgg tatgtaaagt
    59761 atttctagcg acttagtcgg ttgctccatt tagcgtttta tattaactaa gtggtatgta
    59821 aagtaggatt gacacgttac gaccgtgcaa cgttggactg gttttatatt aactaagtgg
    59881 tatgtaaagg caataatatt atcaatttta atattttgtt tctcatgttt tatattaact
    59941 aagtggtatg taaagggttc tggcaagtca cttcatgttg ctagagatat ttgttttata
    60001 ttaactaagt ggtatgtaaa ggttttttct tcgtaacttt gagccttaca tccacaaagt
    60061 tttatattaa ctaagtggta tgtaaagaac aaaaaagata catctgtaac taatttttat
    60121 actgttttat attaattaag tggtatgtaa agacagatat aatgtcaaaa taaggttagg
    60181 acttttaaaa gcatagccat atcagtaaaa atttaattat catttaaaaa ataaattttt
    60241 atttaaagaa tacacataaa tatttccaga ctcaactctc ttaaactatt ttcatatctt
    60301 ttagtagata cctttgttgt tttttaatct ttagcaactt gttcttgagt gaacctctta
    60361 ttttttctaa gttatttcaa gcttttcaaa aaattctcta tttatattca tcacacatat
    60421 aatacaatta attcagtttt aattgccaat atttagtttt tctgtatctg ataaaccaag
    60481 aatataatca gtatataaac caaaaatttt agcaaatatt attaacccat cttctcttgt
    60541 tggtctttca ccagactcta ttctattcat aacacttgta tttatatttg ttttttcaag
    60601 caattctttt tgagaactat tcatattttc cctaatatat ttaatccttt gatttacatc
    60661 catacataat tatctcgaaa agtttctgct ttggaaatat tgttattgaa atatagaaat
    60721 taatgtgaat atatgaaaaa gatacactat gattttataa aaaagaagac atacataaaa
    60781 tttatactta caaagtgttg caaaagagtt aggttttaaa aatgcatcaa cttattttaa
    60841 gtacaggaaa gggtaatatt caattagagt tgatatatta cctataaact atctaagata
    60901 cttgattatg atatagaaaa tttaattaag aggatgataa ggatgaataa tttacaagta
    60961 atataaagaa ataataagag aattttaaat acatagcaac tagcagatat atatgaaaat
    61021 gatgtaaaca atatataaac tagcttcaat agaaataaag agaatgtaaa ttatgtttta
    61081 ttacaattcg agtatttgaa agaatttaag aactaaccta ctaatagtca gttgatttca
    61141 aaacattcta gtcaattata catttggacc gaaagatgtg caaatagaca ctgcaaaata
    61201 ttagatattg ataaaacttg agaacaattt gacaatttag agaaaatata tttcaagatt
    61261 aagcgagaga aactaagctg tatagaagat gtattaatag aaaatttaat atttaaggat
    61321 acaaattaat caagcaataa tatggcatta gaggtaaaaa tagaagttaa aataataaaa
    61381 gatgttgtta tattaaatat aacacattgg aaaaaagaaa caagtctatt aacagttaaa
    61441 attgctagag gtcaaggtga tttaagttat gtaaaatatt ttactaatga gagctaagag
    61501 cttcttaaca agagttttgg tgtgtctatt gaaataatgt taacaattaa taaaagacga
    61561 atggcagaag aaggcgtatg taagtttaaa agagataagt taaacaaact agatactata
    61621 taagaggata agaaacttat agaagaatat gttacttttg taaaggattt agctttaata
    61681 tatagaatat aaagtgattt taagtaaaaa ttgtaacaat taaataagta taagacaagt
    61741 ttatgagttc atattttata acaaggggtg atgttatgaa aatactacaa caatttaaac
    61801 catacaaata tgataaagaa aaaaatatag aatatgatga aaaaattata gctaatcaaa
    61861 acacagaagt ttatatagta tatccacgta tatctgatga agaaaatagg agaagatggg
    61921 aagagttcaa tgaagtagct aaagaagtag ctattaatat agctatgaag aaagttgaga
    61981 cagtagaaag ttaagagaat agagaagttg ctaatactta gcactttctc atagaagaaa
    62041 attggacaac tatttttaaa taattaggaa taaaaaagtt aaatcatatt tgaaaaaagg
    62101 cgataaaatt gttgagtatt gttattagta ataagaatgt attaactatg tattaaaaga
    62161 aattataagt ttttagtaaa tatagtattt aaggatatta aaactggtaa aaaatatcat
    62221 ttagactatt aaataaaaaa gtgctggtca aagtaagcaa cacaataaat aaaaaaactt
    62281 atagaaatta taagataaaa agatgagatt tgaaatggtt gaagttaata taaaaattaa
    62341 aggtgaagta agtgaaataa ttgactcaat tagaggtata tttaatagct ttgatgaaga
    62401 gttgatatta gatataaaat cagagtaaga atataaaggt agatacgtag aaagttataa
    62461 gtaattcaag acttaaatta atttctagtt tttttaatgc aaaaatttaa taaatttcaa
    62521 aatataataa ttctgacatc taaaaagagc tattaaaaat gaataatatt gactattaag
    62581 atatgaatag tgtacatata ttaaattaag atatccagta ggttaatttt atataatgat
    62641 ggggctcaaa ttgaagaggc gatataaaaa tgtattaata tatataaata taagaactat
    62701 aataaatatt aactgtaaaa ttaaatttat agagttatat ttaacattta atgaactgaa
    62761 aaataagtta gcaaataatg aaaaaacaac agtaataaat gagaataaca aagcattttc
    62821 aggattagtg gatttttcta attgagcttt aatcctagtt tgttcaattt cgatatttct
    62881 aatattatta gtattatcaa tttatttgta ataattatca gtttttgcga aatatttttg
    62941 ataatttata gaactttata tattttcctt gaagtatttt tcgcccttat tgtttggttt
    63001 ccaattttaa aactcataaa ctcccaataa attcatgtat aatatacctt aatttataat
    63061 atatttaaga gtttatttca gtaatttttc gaacgatttt gatatatttc atttatagtt
    63121 ttcaaaaact tatacaaaaa agagtgtttc atttttctaa acactctttt tataagaatt
    63181 tgaataataa ttttttaaaa attgataata tggttatttc tttttcaagt ttatagtttc
    63241 ctcatttcca ttccaagata taattagctt gtaactagat tctctatttg aataaagacc
    63301 tggtctttgt atttttattg ctttttcttg acctgcttct aaaatttcat tttttacaac
    63361 tacaactgca tcttcaccat ctataaattg ataggaataa ttaaattttt gttcaactac
    63421 atctttacta tttttatatt ttatttctaa ttttgagtgt ccttcagtag ttacaaattt
    63481 cgctttccaa ttttctgatt ctccaataaa gacattttta tcatttttta tattaagtat
    63541 agctatcaca gagattatta aaactataag aatacctata aaaaattttt tcctcatatc
    63601 aatccctcct ttgaataatt ataacatgag aatagttatt caatactaat tttaaaaata
    63661 ttgtggattt atattctatt acaatagaat gtctgttata acaaataaaa agtagggatt
    63721 aatatgaatt ataggttaaa aaaatctgta acaactattt tatctttact cataatgaca
    63781 atactattac caacaaatgc aagtgctagt aaggtagaag attcatctat gaaagtaatg
    63841 tattcagaga aacaagtaac agacatggac aaattatacg atatggctaa aaatggaatt
    63901 actgatgtta aaagttccaa tgaaaaaggt ataatcaaaa atgcaagaac aggagagtcc
    63961 atatacgttg attctatatc aacaactcaa ttattagaag ttaaacaatc taaaaattta
    64021 acatcaaaga tatattctaa gacagttttt tgatactaat acaataaagc ctcttagaaa
    64081 agcaagtata tcagaagatg gatgggataa aactggtggt gtaaaagcat ctggtacagt
    64141 tacatataca atatcaaaag gagatggagc atttcaatat atttccgtta gtaaagtttc
    64201 tggaaactgg tctattgcag acggccagat tcgtgtaaca aatagaaggg tgcatataaa
    64261 tcaaactgga tttatttcta atgggctagg aattaagcag caacaaaaga aagagccaac
    64321 atcaaattct ttctcatata atgcaccttc tagttggaaa ccagtaggta gagattcagt
    64381 tcaatacaat tgtgggtttg aatcaattgc aacattagta agaggaggaa gttcttggtc
    64441 attcaatttc attgtttaac cttaaaatat tttaagtgaa ttttattaaa attaaagata
    64501 tacatatact ttaaaattag aaaatgtaaa ttaatttgtg tttttaaaga taattcctaa
    64561 gt
//
LOCUS       DAEMGG010000008        61877 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEMGG010000008 DAEMGG010000000
VERSION     DAEMGG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.008"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            98..241
                     /locus_tag="KPK24_000519"
     CDS             98..241
                     /locus_tag="KPK24_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298227.1"
                     /translation="MDDDINMIAIRPEDIKVEFNRDFKDVDIKVREILDIEFRRFNYI
                     VTV"
     gene            579..1646
                     /locus_tag="KPK24_000520"
     CDS             579..1646
                     /locus_tag="KPK24_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2298228.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1838..2368
                     /gene="rnmV"
                     /locus_tag="KPK24_000521"
     CDS             1838..2368
                     /gene="rnmV"
                     /locus_tag="KPK24_000521"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG2298229.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPKSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2538..3407
                     /gene="rsmA"
                     /locus_tag="KPK24_000522"
     CDS             2538..3407
                     /gene="rsmA"
                     /locus_tag="KPK24_000522"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG2298230.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCETEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EEIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3473..4732
                     /locus_tag="KPK24_000523"
     CDS             3473..4732
                     /locus_tag="KPK24_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298231.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKEDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKIENYEDILFQEVEEEEYEELEEEDEEIEDNEESDRDEDSEEEEY
                     IIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKVQE
                     EYIDIEYDEEEEDEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQGE
                     SGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLGY
                     LNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFLR
                     YQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4883..5998
                     /locus_tag="KPK24_000524"
     CDS             4883..5998
                     /locus_tag="KPK24_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2298232.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGKVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAESRSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6184..7080
                     /locus_tag="KPK24_000525"
     CDS             6184..7080
                     /locus_tag="KPK24_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2298233.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7150..7947
                     /locus_tag="KPK24_000526"
     CDS             7150..7947
                     /locus_tag="KPK24_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298234.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVRVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(8002..9354)
                     /locus_tag="KPK24_000527"
     CDS             complement(8002..9354)
                     /locus_tag="KPK24_000527"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG2298235.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9573..10451
                     /gene="purR"
                     /locus_tag="KPK24_000528"
     CDS             9573..10451
                     /gene="purR"
                     /locus_tag="KPK24_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG2298236.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10607..10885
                     /gene="spoVG"
                     /locus_tag="KPK24_000529"
     CDS             10607..10885
                     /gene="spoVG"
                     /locus_tag="KPK24_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG2298237.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11098..12477
                     /gene="glmU"
                     /locus_tag="KPK24_000530"
     CDS             11098..12477
                     /gene="glmU"
                     /locus_tag="KPK24_000530"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG2298238.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYSYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12572..13522
                     /locus_tag="KPK24_000531"
     CDS             12572..13522
                     /locus_tag="KPK24_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG2298239.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14363..14448
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14610..15131
                     /locus_tag="KPK24_000532"
     CDS             14610..15131
                     /locus_tag="KPK24_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2298240.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLNVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGKDGEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGDQLKDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            15282..16958
                     /locus_tag="KPK24_000533"
     CDS             15282..16958
                     /locus_tag="KPK24_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG2298241.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESITIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            16970..18178
                     /locus_tag="KPK24_000534"
     CDS             16970..18178
                     /locus_tag="KPK24_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG2298242.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTPVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILVSAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVSILVVSIF
                     MPMLSIYDAM"
     gene            18193..19905
                     /locus_tag="KPK24_000535"
     CDS             18193..19905
                     /locus_tag="KPK24_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG2298243.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLILDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQLKAKQMKNILK
                     IVPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTADK
                     SSNNETKSNTKSDSNGDKTNNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFEDVKLHSIKSNAEDSTYTFSMTLYLKEGV
                     LTNE"
     gene            19898..20806
                     /gene="pilO"
                     /locus_tag="KPK24_000536"
     CDS             19898..20806
                     /gene="pilO"
                     /locus_tag="KPK24_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG2298244.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVREYEHFYGVSTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTSYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20833..21399
                     /locus_tag="KPK24_000537"
     CDS             20833..21399
                     /locus_tag="KPK24_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2298245.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFMMTIVLSISYKVFNGITSAVKRQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKH
                     TEVYSVTRIQKNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQENHKNSNLNNKTYTFDVMSRIG"
     gene            21435..21962
                     /locus_tag="KPK24_000538"
     CDS             21435..21962
                     /locus_tag="KPK24_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2298246.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSK
                     GDVSLYDTPYEKITTIKDEDGNLIGKENITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            21983..23521
                     /locus_tag="KPK24_000539"
     CDS             21983..23521
                     /locus_tag="KPK24_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298247.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSELEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEHILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPNSQEYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEVDKTEGISIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDSKKNIIGLGVEENSNSKYKVEAIVNFNLNIDTNVVGNYNIKDKILINHDISSY
                     KKVN"
     gene            23621..24703
                     /locus_tag="KPK24_000540"
     CDS             23621..24703
                     /locus_tag="KPK24_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG2298248.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPENIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKSGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24684..25466
                     /locus_tag="KPK24_000541"
     CDS             24684..25466
                     /locus_tag="KPK24_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG2298249.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSIIFNLLFKEIPVKDSIIGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     TSRIKGKEYNSVIPYGPFISIGALITILCGKQLLEFYFSMII"
     gene            25568..26218
                     /locus_tag="KPK24_000542"
     CDS             25568..26218
                     /locus_tag="KPK24_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG2298250.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            26232..26792
                     /locus_tag="KPK24_000543"
     CDS             26232..26792
                     /locus_tag="KPK24_000543"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG2298251.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            26809..30195
                     /gene="mfd"
                     /locus_tag="KPK24_000544"
     CDS             26809..30195
                     /gene="mfd"
                     /locus_tag="KPK24_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG2298252.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30226..31221
                     /locus_tag="KPK24_000545"
     CDS             30226..31221
                     /locus_tag="KPK24_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2298253.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31345..31884
                     /gene="spoVT"
                     /locus_tag="KPK24_000546"
     CDS             31345..31884
                     /gene="spoVT"
                     /locus_tag="KPK24_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG2298254.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            32004..33617
                     /locus_tag="KPK24_000547"
     CDS             32004..33617
                     /locus_tag="KPK24_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG2298255.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33724..35172
                     /gene="mazG"
                     /locus_tag="KPK24_000548"
     CDS             33724..35172
                     /gene="mazG"
                     /locus_tag="KPK24_000548"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG2298256.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            35261..35539
                     /locus_tag="KPK24_000549"
     CDS             35261..35539
                     /locus_tag="KPK24_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG2298257.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35611..35853
                     /locus_tag="KPK24_000550"
     CDS             35611..35853
                     /locus_tag="KPK24_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG2298258.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            35915..36169
                     /locus_tag="KPK24_000551"
     CDS             35915..36169
                     /locus_tag="KPK24_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG2298259.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36170..36703
                     /locus_tag="KPK24_000552"
     CDS             36170..36703
                     /locus_tag="KPK24_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298260.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36709..36993
                     /locus_tag="KPK24_000553"
     CDS             36709..36993
                     /locus_tag="KPK24_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG2298261.1"
                     /translation="MNLRKKFSGQIIVVSLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            37106..38026
                     /locus_tag="KPK24_000554"
     CDS             37106..38026
                     /locus_tag="KPK24_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG2298262.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            38116..40488
                     /gene="spoIIE"
                     /locus_tag="KPK24_000555"
     CDS             38116..40488
                     /gene="spoIIE"
                     /locus_tag="KPK24_000555"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG2298263.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40839..42731
                     /gene="pepF"
                     /locus_tag="KPK24_000556"
     CDS             40839..42731
                     /gene="pepF"
                     /locus_tag="KPK24_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG2298264.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42845..43810
                     /gene="galU"
                     /locus_tag="KPK24_000557"
     CDS             42845..43810
                     /gene="galU"
                     /locus_tag="KPK24_000557"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG2298265.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            44310..45914
                     /gene="rho"
                     /locus_tag="KPK24_000558"
     CDS             44310..45914
                     /gene="rho"
                     /locus_tag="KPK24_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG2298266.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            46067..46267
                     /gene="rpmE"
                     /locus_tag="KPK24_000559"
     CDS             46067..46267
                     /gene="rpmE"
                     /locus_tag="KPK24_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG2298267.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            46412..47305
                     /locus_tag="KPK24_000560"
     CDS             46412..47305
                     /locus_tag="KPK24_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG2298268.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            47323..48171
                     /gene="prmC"
                     /locus_tag="KPK24_000561"
     CDS             47323..48171
                     /gene="prmC"
                     /locus_tag="KPK24_000561"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG2298269.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYVENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            48215..49279
                     /gene="prfA"
                     /locus_tag="KPK24_000562"
     CDS             48215..49279
                     /gene="prfA"
                     /locus_tag="KPK24_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG2298270.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            49408..50088
                     /locus_tag="KPK24_000563"
     CDS             49408..50088
                     /locus_tag="KPK24_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG2298271.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            50117..51157
                     /locus_tag="KPK24_000564"
     CDS             50117..51157
                     /locus_tag="KPK24_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG2298272.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            51174..51623
                     /locus_tag="KPK24_000565"
     CDS             51174..51623
                     /locus_tag="KPK24_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG2298273.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            51640..52092
                     /gene="rpiB"
                     /locus_tag="KPK24_000566"
     CDS             51640..52092
                     /gene="rpiB"
                     /locus_tag="KPK24_000566"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG2298274.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            52128..52757
                     /gene="upp"
                     /locus_tag="KPK24_000567"
     CDS             52128..52757
                     /gene="upp"
                     /locus_tag="KPK24_000567"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG2298275.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            53058..53873
                     /locus_tag="KPK24_000568"
     CDS             53058..53873
                     /locus_tag="KPK24_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG2298276.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDTVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            54099..54536
                     /locus_tag="KPK24_000569"
     CDS             54099..54536
                     /locus_tag="KPK24_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG2298277.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            54721..54948
                     /locus_tag="KPK24_000570"
     CDS             54721..54948
                     /locus_tag="KPK24_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG2298278.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            54953..55339
                     /locus_tag="KPK24_000571"
     CDS             54953..55339
                     /locus_tag="KPK24_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG2298279.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            55342..56046
                     /gene="atpB"
                     /locus_tag="KPK24_000572"
     CDS             55342..56046
                     /gene="atpB"
                     /locus_tag="KPK24_000572"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG2298280.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            56115..56375
                     /gene="atpE"
                     /locus_tag="KPK24_000573"
     CDS             56115..56375
                     /gene="atpE"
                     /locus_tag="KPK24_000573"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG2298281.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            56498..56995
                     /gene="atpF"
                     /locus_tag="KPK24_000574"
     CDS             56498..56995
                     /gene="atpF"
                     /locus_tag="KPK24_000574"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG2298282.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            56992..57537
                     /locus_tag="KPK24_000575"
     CDS             56992..57537
                     /locus_tag="KPK24_000575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG2298283.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            57554..59056
                     /locus_tag="KPK24_000576"
     CDS             57554..59056
                     /locus_tag="KPK24_000576"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG2298284.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            59084..59962
                     /gene="atpG"
                     /locus_tag="KPK24_000577"
     CDS             59084..59962
                     /gene="atpG"
                     /locus_tag="KPK24_000577"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG2298285.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            59975..61369
                     /gene="atpD"
                     /locus_tag="KPK24_000578"
     CDS             59975..61369
                     /gene="atpD"
                     /locus_tag="KPK24_000578"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG2298286.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            61372..61632
                     /gene="atpC"
                     /locus_tag="KPK24_000579"
     CDS             61372..61632
                     /gene="atpC"
                     /locus_tag="KPK24_000579"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG2298287.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        25030 a         6614 c        11482 g        18751 t
ORIGIN      
        1 aacttaaaat tggaagtaag aatatttaac tagagaagaa ttatttaagt tcaggttagc
       61 tctaggagta gctgatttta taggaacaat agattttatg gatgatgata taaacatgat
      121 agcaattaga ccagaagata taaaggtaga atttaataga gatttcaaag atgttgatat
      181 aaaagtacga gagattttgg atattgaatt tagaagattc aactatatag ttacagttta
      241 atatcgttct aagcaaataa agttgaatgt agtttcaaat gtagtggaac aaatggaact
      301 atgtgtaggt tataaaataa acttcaagat acctaaagaa agaatcatat aatataagta
      361 atccattaat ttttttatag acctcaaact cagaccgcat cgatataaaa actaaagcta
      421 gatgatatag tgttgtatat aggaccatca gctgttatgg cagtattagt gcttttaaca
      481 aatataatag taaaactttt gtatgaattg ataataaaaa aattaaaaac caagacataa
      541 aaatggcaac aaaaagaagt tgaataaggg aggtaaaaat gaaatttaag aagttagtct
      601 ctttgggaat attatcaaca cttttattga gtgtaataac tggatgtagt aatgtttcat
      661 caggttcaaa cttaaagtct aataataaga caaaaggaga gttaacagtt tatacagcaa
      721 ttgaggaaga tagtatagat acatacttaa ctacatttaa agagaaatat ccagatatta
      781 aattgaatgt acttagatta tcagtaggag gtataacagc aagattatta gcagaaaaag
      841 ataatcctaa agcagatgtg ttatgggggg ttgcagctac aagtttgata attttagatg
      901 acaaagaaat gctagagcca tacgctccga aaggctttga taaggtaaat cctgcattta
      961 aagatgataa gaaagttcct tcttgggttg gaatagatgc ttggatgact ggtataacag
     1021 taaatactaa agaattaaaa gctaaaaata tatcagctcc tagttcttat gaagatttaa
     1081 taaagccaga atataaagat cttatatcta tgcctagtcc agcttcttca ggaacaggat
     1141 atttaacagt atcggaactc atacaaatta tgggtgaaga aaaagcatgg cagtacatgg
     1201 ataaattaca taaaaatatt ggtgtataca ctcagtctgg ttcagctcca gctatattag
     1261 cagcttcagg agaatatcct attggtatat cttttggaaa tagaggtata aagttaaaag
     1321 gtgaaggtta tccagtagaa gtggtatttc caaaagaagg ttctggatgg gagatagaat
     1381 caaatgcact tgtgaaaaaa gataatataa aagaagcatc aaaagtattt ttagattggg
     1441 ctataagtaa agatgcaatg aatgagtatt ctaagaattt tgcagttact acaattgaca
     1501 cgggaaatcc agtgccagaa ggatttccaa agaatccctt agaacagatg atagataatg
     1561 atttaaaatc agcagcaaga aatagagaag ctatactaaa taaatggata gaaaaatatg
     1621 atggaaagac agaaaaagag agctaagctt tacaataaaa tttagtataa aaagcgccta
     1681 gaatataaat tttatatcta ggctcttttg atatatttat ttaaaatgca tatttctata
     1741 taaaaataaa tagtttagta ccttgattaa attaaataaa aaccttaaaa taaagagatg
     1801 taaagaaaaa ctaaaaacga ataaaaggtg aatttatatg ataaaagaaa taatagtagt
     1861 agagggtaga gatgacgtca cagcagttaa gagagcaata gatgcagaac ttataactac
     1921 aggtggattt ggatttccaa aaggtgtaat ggagcgtata aaagctgcta atgaaagacg
     1981 tggtgttata atatttacag acccagattt tgcaggtgaa aaaataagaa aaaaaatagc
     2041 atcagaagtt ccaggatgta aacatgcatt tctaccaaga gaggaagcta aaaaggatgg
     2101 agatataggt attgagaatg ctactcctaa aagtataata gcagctttga ataaagttag
     2161 aactgaaaat acagaaaaaa gagatgaatt taagcaagtt gacttaatta gaaatggtct
     2221 tataggtaat gaagaagcat cacttagaag agatgcactt ggaaaaatac ttggaattgg
     2281 atatggaaat gcaaaacaat ttttaaatag attgaataat tatggagttg aaagagaaga
     2341 atttatagat gcattaaaac aaatctaaac gtaatgttta atgtatgaaa aatatgtata
     2401 taaaataaat tgtggaatta cggttaattt aagataaatt atttataata tataccttat
     2461 tttaaatcaa tgtaaataca tataataagt ataattaata aaattaaata tcaaaataag
     2521 ttaaaaagga gtaaataatg gatagactat cttcacataa tgcaacaaag gaagtagtac
     2581 aaaaatataa ttttaaattt tcaaagtctt taggtcaaaa tttcttgata gatagtaata
     2641 taattgataa aattttatcg ggagctagaa tcacaagagg agataatatc atagaagtag
     2701 gacctggtat aggaactctt actcgtgaga tgggtaaaat agcagaaaaa gtagtggcta
     2761 tagaaatcga tagaaattta attcctatat tgaaagatac cttgtcagat ttagataata
     2821 cagaggttgt aaatcaagat atactaaaag tagacataca ggaattagta aaagataaat
     2881 taaatggtgg accagttaag cttgtagcaa atcttccata ttatataact actccaatag
     2941 ttatgaaatt tttagaggaa gatatacctg taacagatat agtagtcatg gttcaaaaag
     3001 aggttgcaga tagaatgaat gctattccag gtactaaaga ttatggggca ttatctatag
     3061 ctgttcaata ctattgcgag acagagattg tggcaaaagc gccaagacat atgtttatac
     3121 cacaaccaaa tgtagattcc acagttatag gtttacatgt aagagataaa agaaaatatg
     3181 atgttcataa tgaagaaata ttttttaaaa cagtaaaagc atcctttgga caaagaagaa
     3241 aaactcttct taattcgttg ggaggtcttg gttttcttaa taaagatgaa ataagagaaa
     3301 tattaaaaga agctaatata gacgaaaaaa gaaggggtga aactcttagt atagaagagt
     3361 tttcagttct ttcaaatata ataaatacaa aagtatcttc taaatagaag gtactttttt
     3421 ttattttgtt aacatatatt gtttagagaa actgtgttaa ggggggattg cagtgggttc
     3481 taaaagaaag tttaaaagag actatataat actagaagct aaagacataa actttagata
     3541 caaagaacga gtactgccaa aagcttttgc aaaagttgaa gtaaatgatg aaaaagcaat
     3601 tattgcatta tatgtagaaa atctaaaata tgttaaagat gggtataaag cagtagcaat
     3661 aaaagaagac catggattaa tagacttagg aaatataatt ttaaatgaac aaggaagagg
     3721 tgaatttgtt ttagatttag aagattatga tgatgagata aaaggtattg cattattgta
     3781 tgaaagaaat atacctttaa ttggatttaa gggaaataag attgaaaatt atgaggacat
     3841 attatttcaa gaggtagagg aagaagaata tgaagagttg gaagaagaag acgaagagat
     3901 agaggataat gaggaaagtg atagagacga agatagtgaa gaagaagaat atattataga
     3961 agaatatgat gaagatgagt atgaagacta ttgggaagat gatgaagata ttgaagaagg
     4021 agagttaaag gcaagaacgg aagtagaaga ttctggtgat gaggaaatag aagagttcaa
     4081 taattataag gaaatagaaa attataatag ggttaaagta caggaagaat atatagacat
     4141 agaatatgat gaggaagaag aggatgaaga agaagttttt gaagtaaata gagaagaaga
     4201 caggtttgtt tcctcaaaac ctactaaatc aaagaaagtt caaaaagatc agaataatat
     4261 tgaaaaaata aaaagagcta atggagtaag tcaaggagag agtggaaata aacaaacaag
     4321 tacattactt atgccaagac aaataaaaaa aggtttgaaa ttatttaaag aagtaaaacc
     4381 ttttgtaaca gattatatag ataagacaag atggtggaaa atagagataa atcctacaac
     4441 tttgtgtgga tatacaatgc cttatttagg atatttaaac gtgctgaatt acaccatgta
     4501 tagtgatgca gttttacatt catataaata cagacactat ttatttggag ttcagtatga
     4561 tgaatataat aagagaaaac attatattta tgctatccca ggaaatagaa atgaacagcc
     4621 agacaatggc agcacaggat ttttaagata tcaagcttgt gataatagaa caaatagttt
     4681 aggatattgg ctatgtttta tagattctag acaaagaaat atagttagat aaaaataata
     4741 aaaatgaaaa aatttagact caattcttat tttactgaaa aaattaatga aatgtagtac
     4801 aaaactttaa aaactattga taatatttaa atattacact acaatatatc ttgaaatacc
     4861 tattatatta tggaggaaaa ttatgataaa cgagcaaaga attttaaatg agtttcttga
     4921 attagtacag atagatagtt tatctttaaa agaggggaaa gttgctaaag tattagttaa
     4981 aaaattagaa gaaataggtt gttcagtagt aatagacaat gctggtgaaa aagcaaatgg
     5041 agaaactggt aatataatag ctacattaaa gggaaataaa gaaggaaaga aaatattatt
     5101 tagttcacat atggatacag taactccagg tataggagta aaacctatag tggatgaagc
     5161 aaatggaata ataaaaagtg atggtactac aatattaggt tctgatgata aagcaggtat
     5221 agcagctata ttagaaggct taagatatat aaatgaaaat aacatagaac atactgatat
     5281 acaagttgta ttctctatat gtgaggaatg tggattagtt ggagctaaaa atttagacta
     5341 tggaaaaata gattctgaat atgcgtttat attagatagt ggtggttctc caggtgaaat
     5401 aatagtaaaa gctcctgcac aagatgtaat aaatgttaag atattaggaa agactgctca
     5461 tgcaggtctt gaacctgaag ctggaataag tgctataatg gtagctgcca gagcaataga
     5521 aaatatgaat ttacttagaa tagacgaaga aacaactgct aacataggaa ttataaacgg
     5581 tggaacagct acaaatatag taacaggaga agttaatata gttgcagaat ccagaagttt
     5641 aaaagaaaat aaattagatg ttcaaactaa acacatggta gagacttttg aaaaagctgc
     5701 aaaagatttt ggagctcaaa ttgagataga tgtcaataga gcatatacac caatagatgt
     5761 tagtgaagat tctgaaataa taaaacttgc aaagaaggct ttttcaaact taggtataga
     5821 aggtcatact gaatctacag gaggaggtag tgatacaaat atattaagta agaatggtat
     5881 cgaagctata actttaggaa tcggtatgaa aaatgctcat acactaagtg aacatatagc
     5941 tataaaagac ttatacgatt ctgctaaaat ggtagtggag ataataaagg aagcataaat
     6001 ttaaataata gtgtataata tatatatcgc ctagcttaag ctaggtgatt tttcatttta
     6061 tagaagattg ataatctcta ttgaaagtaa agagtataca tttgcatata agagagagtt
     6121 taatttatgt tcagatgtag gttcttaata atatttagat tgtttcataa gggaggaata
     6181 aatatggaaa caaggtatga agaagcaaat aaaataacaa tacaatcaat actttggaat
     6241 gtagtattaa caataattaa ggttatagct ggtgttattg gtaattctag cgctatgatt
     6301 gcggatggat tacattcagc atcagatatt ataagttcta taggagtact aataggcaat
     6361 tatgtatctt ctagacctgg agatagagag cataattatg gtcatgagaa agcagagaca
     6421 ttagtatcat ttgtgttatc aatattgctt attttcgttt ctataacaat aggtatagag
     6481 gcaattaaat ctttatttaa cttagatgca ttaagtgttc catcaatatt accattagta
     6541 gtatcagtta tttcaatcct gataaaagag tatcaatata gaataacaat aaaagttgct
     6601 aaaaagataa attcaccagc cctaaaagct gatgcttggc atcataggtc agatgcttta
     6661 tcttcagtag ctgcttttat aggtatagga ggttctattt taggatttaa gccattagac
     6721 ccaattgcat cagttgtagt ggctatattt gtagctaaag taggaattag tatacttata
     6781 agttctgtta atgaattgat ggatgtttca gttgatgaag aagagataaa agaattaaaa
     6841 tttattgtag cagatacaga aggtgttaaa aatcttggtg atataaagac tagaaaacat
     6901 ggagctatgg catatgtaga tttgactata tgtgtggatg aaaatttgac agttaagcaa
     6961 ggtcatgata ttgctactaa gcttgaaaaa catataataa agcatatgga atttgtaaaa
     7021 ggcataacag ttcacgttga accctgtact aattgccaag gaaataagtg taataattaa
     7081 ttaaaactta aaaaaaagtg taaaaagtta tataattgtt ttatgattat aaactctagg
     7141 ggtggttaaa tgagcaagaa aagaaaacta aagaaaaaag ctgcaatatt aataggagta
     7201 gttgttttct ttataatata ctttatatcc tttagaatag gaataaatat ggaaaaggat
     7261 aaaagtgaag tgccaaagaa ggtagaaaaa gaagaagaag ataataggtc tttaaggact
     7321 caaataaaag aaaagaagaa ggtttatata tcagacaaaa atatagaaaa cataaaaatt
     7381 gaagaagata tgtgggatga aataaagtta tttttctcag attttaagaa agtaaggaat
     7441 actacttctt cttacgaagc agtttatgaa ggttattctg atgatggagt taaattttct
     7501 actgacctta acttttttag agtatatact gtaaataaag aagaatatta taaagtacca
     7561 gttagttcta aggatgcttt taagaaactt ttagatgaga gtatatatac ttcgtttgac
     7621 cttttaagtc attataaaga ttggaaagag gttactataa ctcatggaaa agaaattaaa
     7681 aaagttcaca gatggaaatt tgatgattta gcacataaga tgatttcaaa aagggttgta
     7741 ggaaaagttc aacctgagaa aagtaaggaa agaagtgact ataattttac tataaatata
     7801 aaaggtgata attttgatgt aagagttgat acaatgggaa aagattatgt aaaagttact
     7861 tctaagaaaa atgtatctta ctatgaggtt catacaagtt tatttgatta ccttaaaaat
     7921 gagatatttg aaattggaaa gaaataagac tttaagaagg ctatcaaatt agatatttga
     7981 tagcctaaag tattttatat attatttttc aaccaataag tctgcaacct tataaattgg
     8041 accagcacca gcagttatta ctaaatcatt gtccttaaca ttttcacgta agtattttac
     8101 tatatcttca aattctttta tatagatggc atctacattg ttctgatata atttctcaac
     8161 caagtcctta gaatgtatat ctcctgggtc tttttcccta gcagcatata tatctgttat
     8221 gataacttta tcagcagcat agaatgcctc tgaaaattca cctaaaagag attttgttct
     8281 agtataagta tgtggctgga agatacacca taaagtggat ttttttaatt ttttagcagc
     8341 agctaaagta gcttttaact cagttggatg atgtgcataa tcatctacta ctaaagcatt
     8401 cttatagtac cctttagttt caaatcttct tccgacacct ttataaattt ttatattttt
     8461 tcttatagtc tctaagtcaa taccagaaac aagagctgcc attatagcag aagaagcatt
     8521 gtatatgtta tgaagaccat atacagaaag ttcaaattct cctaaatctc ttccttcata
     8581 ttctattcta aatattccat gaccactatt gtcaaagtga atatctttta ttacagcatc
     8641 attgtcagaa tctctaccat attttataat agtagcttta acatcataca atatgtcatc
     8701 aatattttca tcatcgccat ttattatgaa atagccttct gggggtaata attttccaaa
     8761 tttgttaaaa gaagccttga tttcatctat tccagagaaa taatcaagat ggtcttcttc
     8821 aacatttaaa actatagata tttttgggtt aaaattaagg aaactatcta catattcgca
     8881 tgcttcagtt ataaagtggt tggaattacc tatcttaaca ttaccaccta tcatacttaa
     8941 gttaccacca actagtatag ttgggtctaa gtctgcatat tcaaagattg tagataacat
     9001 agatgttgta gaagtttttc catgtgttcc agaaactgct atagaatttt tatattccct
     9061 catgatttgg cctaaaaaag tcgctctgtt cattaccaat ttattttttt ctttagcagc
     9121 cacaagttct tcattgtctg gatggactgc tgctgtataa acaaccatat ttacatcatc
     9181 tgatatatta tcttttttct gaccaatata aatagttgcc ccttggtccc taagtttgtc
     9241 taataggtat gattcgtttg aatccgaacc tgaaacttga taacctttat tgatacatat
     9301 ttcagctaat gcactcatgc tgataccgcc aattcctata aagtgtatat tcataataaa
     9361 accacctttc tttttaatta agtcctacaa tatgaactat ttctgtggta taataatata
     9421 atgttcaata ttttataatt aatatgcata taatattata tgtattttat ttttgaatat
     9481 aataaaacat aagtaatatt ataccacaat aagaattgtt atgcattagt tagtttaacg
     9541 ttatataaag gtacttgtag gaggaaataa taatgaaatt taaaagaaca gagagaattg
     9601 gagcaatagt taaaatactg tctgataatc caaataaaat atatacttta agttatttta
     9661 caaatcaatt taatgcagca aaatcaacaa taagtgaaga tttattagtt gtgaaaaatg
     9721 tatttgagaa gctacattta ggtaaagtta taactatctc aggagcagca ggaggagtaa
     9781 aatatatacc taaaacttct atagcagaaa atgcagagtt cttaatggaa ttatgtgaga
     9841 gaatatgtga caagtctaga atactatctg gtggtttttt atacttgatt gacctaattt
     9901 acgaccctac aatagctgct aagataggaa agatattcgc atcaaatatt gagtatgtag
     9961 atgcagatta tgttgtaaca atggaaacaa aaggtatacc tatggcactt atgactgcaa
    10021 aagctatgaa tttacctcta gttataataa gaaaagatat aaaggtatca gaaggtccta
    10081 cacttagcat gacatatgtg agtggaaaca gctctaaagt tgaaagtatg agtttgccaa
    10141 gaaaagctct taaaccagat agtaaagtta taattataga tgatttcatg aggggtggag
    10201 gtactataaa aggaatggta gaccttatga atgaatttgg tgcagaggtt ataggtactg
    10261 gagtattcat atctacaacg aatccttcag aaaaaatggt aaaagattat atttcattga
    10321 ttcaattgga tgttaatgga gataaaatag tagtagaacc aaacttaaag acttttaaag
    10381 atgaatatag aaatgaagat ttagacgaag aagaagattc agaatttgaa tttgagatag
    10441 atgaagatta ataaaaaaaa tatttagaaa aatatgtaaa aataaaaaga ggatatccct
    10501 agttgttcat agaattattt aaaaaacagt ttatattaat ttattccagc gataataatt
    10561 ttaatataaa ttaatttctg tcaaaatcaa agggggatat tttggtatga agataactga
    10621 cgtaagagta agaaaattaa cagaagaagg taagatgaaa tgtatagttt caataacttt
    10681 cgataatttg tttgtagtgc atgatataaa agttatagaa ggccataatg ggctatttat
    10741 agcaatgcct agcagaaaag taggcgaagg aaatttcaga gatatagctc atccaataaa
    10801 tgcagaaatg agacaagttt tagaagatgc agttttacaa gcatatcatg aagcattagt
    10861 tcaatgggaa gtagctgctg aataataata aatatataaa atggagagag cccgaacatt
    10921 taggctcttt ttaaatgtag aatttctatt aaaatatgta ctttttagta aaaaagtgct
    10981 atattattag tgataatgta tatgttgtat acaaattgtt agataaatgt attataatgt
    11041 atattgatta agagaataat acgaataaaa ttaaattaga ttgaatggag tgtatcaatg
    11101 aactttaaag ctataatcct tgctgctggc aaaggaacaa gaatgaagtc taaataccca
    11161 aaagttatac ataaagtatg tggaaaagaa atggttaacc atattataga tgtttcaaaa
    11221 aaatcagggg taaaagatac tgttgtgatt ttagggcatg aagctgatgt agttaaagaa
    11281 aagcttgcag aagaaattat aatagccatg caaacagaac aacttgggac aggacatgct
    11341 gtaaagatgg ctaaagaata tattaatgat gaagatacaa tagttgtact ttgtggagat
    11401 actccactta taaaagaaga gacactaaag agattatttg aatatcatat agaaaataaa
    11461 tatcatgcca cagttcttac aacaagagtt ggaaatccaa caggttatgg tagaataata
    11521 agagataaga agggtgacct tcttaaaata gtggaacaaa aagatgcaaa ttcagaagaa
    11581 aagatgataa gtgagataaa ctctggaatt tattgcttta atggaaagag tcttagagaa
    11641 gcactagatt tgctaaataa taacaattca caaggtgaat attatttaac tgatactgct
    11701 aaaataatga gagataaagg attaaaagtt ggtgcatttg caggttcaac tattgaagaa
    11761 cttatgggcg ttaattcaag agtagaatta tcaaaggcag aagaaattat gagacgaaga
    11821 ataaatgaat ctcatatggt aaatggagta actataatag acactaactc tacctatata
    11881 gaatctgatg ttatgatagg caatgatact ataatatatc caggtgttat gttacaagga
    11941 aaaacaagga taggttcaga ttgtataatt ggaatgaatt catctataac aaattcagaa
    12001 ataggtgatg gtacagaaat aaaaaattca actataattg atagtaaggt tggagaaaat
    12061 tctactgttg ggccatattc atacttaaga ccaaagagtg acttaggaaa taatgtaaaa
    12121 ataggtgatt ttgtagaagt aaaaaatgct attatagaag atggctcaaa agcatctcac
    12181 ctttcttata taggagatgc acatgtaggt aaaaatgtaa atattggatg tggagtagta
    12241 tttgtaaact atgatggtaa aaataaattt aaatcagtag taaaagacaa tgcgtttata
    12301 ggttcaaatt ctaatttagt tgcaccagta gttgtagaag aaaaaggata tatagcaaca
    12361 ggttcaacaa taacacatga tgtcccagat ggtgctttgg caatagcaag agaaagacaa
    12421 gttatcaaag aaggatgggt agaaaaaaag aatcaaaaag acgaccaaag taagtaatta
    12481 tgtagtttat tattttggct aaaaaattta gctcgaaatt attataaatt atattcatta
    12541 gtattttaaa attttcagga gggttacata aatgaatact agcggaagcg agattaaaat
    12601 tattgcaggt aattcatcga aagagttagc acaaaaaata gctgactata taggtgtatc
    12661 agtactagat tgtgaagtag gtacatttag tgacggtgaa atatgtgtaa acatgaatga
    12721 gacagtaaga ggttgtgatg tgtttgtagt gcaatctact aacagcccag taaatgataa
    12781 cttaatggag ttattaatct taattgatgc gttaaagaga gcatcagcag gtagaataac
    12841 agcagttatt ccttactatg gatatgcaag acaagacaga aaggctaagg caagagatcc
    12901 aatcactgct aaattagttg caaatttaat aactgctgct ggagcagata gagtattaac
    12961 aatggattta catgcagctc aaatacaagg atattttgat ataccattag accacttact
    13021 aggtggaaca atattagcaa attattttaa tgaaaagaaa atagaagatt tagtagtagt
    13081 atctcctgac ttaggaagtg ttactagatc aagaaaattt gctaatactt taaatggaga
    13141 agttccaata gctataatag ataaaagaag accgaaagct aatgtatgcg aagttatgaa
    13201 cttaataggt gatgttaaag gtaaaaatgt tatactatta gacgatatga tagatacagc
    13261 aggaacaata gttaatgctg caaatgcact taaagagttt ggagcaaaag atgtttatgc
    13321 ttgttgtact catggtgtgt tatcaggtcc agctattgaa agaattgcta attcagaaat
    13381 aagtgagtta atagttcttg atactataca acttcctgaa gagaaaagaa tagataagat
    13441 aaagataaaa acagtagctc ctttatttgg agatgctata agaatgatat tctctaatga
    13501 atcagtaagt aaattattct aatttattgt atttagaaga gttgtttaaa aggctgtaaa
    13561 ccttttgagc aactcttttt tgattttata cttatgaaat gctatttttc tcttgttaga
    13621 aaggtatata ttattgtaga aagaaattat agtttaaaat tatgtatatt taagcaaaat
    13681 aaacttaata aagggcatat tattcataat attgtgaata taaaaaatat tacaaaaaag
    13741 tattgaaaaa attcagatta tgtagtatta tgtaaatata aataaagaaa gaggtgtttc
    13801 caatgggaag gttagaagat gaacccctaa taattaaatg attcagtcac aatctaaagt
    13861 gaatataaaa atctattgaa agtttattta taagaattga aaatagtatc tattctatct
    13921 ttagtgtttg tgagagggtt ctggattata taagattact gtaaagaagt aattttgaac
    13981 aataagcata tctgtaaatg aagagtttcg tttatataat gattgtggct gaatcataac
    14041 tataatagat agaatgttta taatagatga ttttataaaa tactatattg taatagcttt
    14101 tagctttcaa ataaatatta gcaaaggatg gaaatgacaa tgataaaact aaatgataat
    14161 ttataaatat ctccttacga tatataataa ttgtagggag atgtttcttt ttttgtatag
    14221 tataattttt tctagaaatt ctgtttcaat atgtaaaaag ttggtatata taaatagtat
    14281 aaactgtgtt gctaaaatat ggtagatatt atataatata agtggatatg tatatacatt
    14341 atgagttata taattttgaa tttaataaaa tagaaacgtt gatttatgtt ctgtaatgtg
    14401 ggcaccttgg agcatattga gttagtggtg caaccggcta tgaaattgta tttatttata
    14461 gatactatta ttttcatagc tttttttatt taaaaaatgg ttatatgtta gaaataaatt
    14521 tggttagtac gtttaaataa ttttacttag atttttaacc agatatatta taagtaagta
    14581 ttaattaaaa aatttaaggg ggaataaaaa tgaagttaaa aaagaataaa aaaggtttca
    14641 ctttagtgga attattggta gtaattgcaa ttataggtat attagcagta gtggcagttc
    14701 cagctttatt tagtaatata aacaaggcta aggtagcaag tgttgagtct gattatagtt
    14761 cagttaagag tgcagcatta tcttattatt cagatactaa taaaatacca gttacaccag
    14821 atggtcaaac tggtttaaat gttttagaga cttatatgga atctcttcct gataaagctg
    14881 atataggtgg aaaatataaa ttgattaaag ttggtaataa attagtatta cagataggta
    14941 aagatggtga aggagttacc ttaacagaag cgcaatcagc aaaattattg agtgatatag
    15001 gtaaagataa aatatataca ggtgttacag gagataattt tggagaccaa ttaaaagata
    15061 ctacaaaaat agataataaa gctctatata tagtacttat agataatact gtgatggact
    15121 caacaaaata gttaaaacaa tatctttata aaaatattgt aaatttaatg aaaaatataa
    15181 tataaaagac aggaagaaaa tcttcctgtt tttttatact attttcttta atgttcaatt
    15241 atgaatgatg atataattga gatagtaaag gagtgaaacc tgtggctaaa aaagttagaa
    15301 taggagacaa actggtcgaa aaaggatata taacagaaga ccagcttaaa tgggcactat
    15361 cagaacaaaa aaacagtgga aaaagactag gagaatttct agtacaagaa gggcttatag
    15421 atagtaactt actaataagt gtattaaaag aactcttgga tatagaaagt atatttttag
    15481 aagggacaga aatagacact ttagctacaa agatggttcc agaaaatata tgcaaaagat
    15541 atactgtatt tccatttaaa atcgatggaa ataaaatatg tctagccatg tcagacccac
    15601 aagatagaga agcagttcaa gatgtaaggc gtatgtctgg taaagacgta gagatattta
    15661 tatcaagtac agaagatata aacaaagcaa taggtcatgc ttatgcacat tcagaaataa
    15721 ataaggcaat gactgagtac aacaaaaata gaactggtgg agtaagagaa acagtaatac
    15781 ttgaagaaga tgtaaatgct gctccaatag tacgattagt aaataacata ctagaaaatg
    15841 ctgttagaat ggaagcaagt gatatacata tagaacaaag tgaaaattac atgcgtgtta
    15901 ggtttaggat agatggaatg ttaagagaat atatgaggat gaactcagca ccatataagg
    15961 cagtaatcag ccgtatcaag ataatgtcag atataaatat atctgaaaag agaataccac
    16021 aggatggtag aatctattta aaagtagata ataaacccat agattttagg gtttctacaa
    16081 tgccaactaa cagggatgaa aagatagcta tgagggttct tgataagagt aattttatgg
    16141 ttagtaaaga agtactagga atagacgaac atggctctaa gatatatgat gaattgataa
    16201 acactcctta tggtcttata ttggttgttg gccctactgg aagtggaaaa accactacat
    16261 tgtattcaat gttaaatcaa ctaaacacag aaaatagaaa cctattaaca atagaagacc
    16321 ctatagagta tgaattacct ggtgtaaatc aatctcaaat aaatgaaaag gcagggctta
    16381 ctttcgccag cggacttaga gcttttatgc gtcaagaccc agatataata atggttggag
    16441 agataagaga tactgagaca gcagaaatag caattagagc ttctcttaca ggacatttag
    16501 ttttaagtac attacatgca aatacagcag ttggagcaat ttcacgtctt ttagatatgg
    16561 atgtggaaag ttttttaata acttcatctg ttttaggtgt aatatctcaa agacttacac
    16621 gaaaaatatg tgagcactgt aaggtttcat atgaagcaga tattggagaa aaaaaggctc
    16681 ttggtattga tgtaaatgaa tcaataacta tttatagagg aaaaggttgt gaaaggtgta
    16741 ataatactgg atataaagga cgtctaggta tatttgaaat gcttgaaatt actccagaaa
    16801 taaaagagtt aatagattca tctgcaaatc aaagagaaat tttaaagatg gctagaaaac
    16861 aaggtatggt tagcttaaaa gaggatatag ttaaaaaagt tttaaatgga aagacaacag
    16921 tagaagaaat gattagaata attttgatga ctgattaaag gatgatatta tgaaaagttt
    16981 taaatacaca gtaataaaag aggacggaaa aaagcaaact gatgtgattg aagccaatga
    17041 ttttaatgaa gctagaaatc ttcttagaaa gagaaatctt agagttattg aaatcaaaga
    17101 aagcaaaaat aaaaatataa gattatctag tagaaagaga aaaaaagact tgggagctga
    17161 ccagataagc cacttttgca gacagtttgc tattatagta tcttctggta taaatagtat
    17221 ttctggatta gaaacattag ctagaaggtc aaccaacatt acattaagag aagaaattaa
    17281 tcgtatagtg gcagaaataa aagtaggttc tactattgca gattcaatgc tttcatcaaa
    17341 atcaaagttt ccaaagttac tcggtgctat ggtagctaca ggtgaagcta caggtaaatt
    17401 agatgaagta ttaaaaagta tggcttcatt ttatgcaagt gaacacagag ttaaacaaaa
    17461 gcttagaaat gctgcaacat atccattaat agttttgatt tcttcattta taatgatttt
    17521 tatatttaca acatttatgg tacctaagat gattaattca attacaacag taggagcaac
    17581 tcttccactt ataacaagaa ttgttatggg gtttggtatg tttatgaaga agttttggat
    17641 aatagtactt ttagttatta tattatttat ctatcagttt aaaaaatatc taaaaacgcc
    17701 agttggaagg gctcataaag atagagttat aaacaagata cctgttttag gaaaaggaat
    17761 aaattgtatg gtagctacac gattttcaag agcattgtat ctatttgtat caacaggata
    17821 cccattagtt caagggcttg attatattat agatagtgta aataacacca tggcagaaaa
    17881 gattttagta tcagctaaag atggaataac aagaggagaa ggtttagcag aaaatttaga
    17941 aagatatacg tattttgact ctgtattggt acagatgata gctatagggg agcagacagg
    18001 ggaattagag aatataagta gacagatggc cgaattttat gagtatgaat ccgagattta
    18061 tttaaatagg atggcctcaa tgatagagcc tgttttaata attgcagttg gtattatagt
    18121 ttcgatactg gttgtatcaa tatttatgcc aatgcttagt atatatgatg ccatgtagga
    18181 gggaaatgac aaatgaacaa ggtttatgta tcatattatg gcgattctat ttccatagtt
    18241 gagggcagat ataaacacga taaggataag ttttatgtaa aaaattttaa tgtgttttca
    18301 atagaggata ttgcacaaga tttttctaag gaaaagtatg ctctattgag atacgcatta
    18361 gaaaatgtta agttaaaatc aagacatgta gttttttgtt taaatacaag agatgtaatt
    18421 ttaaaacccc aaaaacttcc taatatggat aaaaaggatt tagacggatt tatgaagcta
    18481 gagatggacg aaatgatggc tttggagtca gatgaatacg tattttcgta tgaagtatct
    18541 agtgaaacac ctgataattc agatggcgat gaatcaagct taaatcttat cattggagct
    18601 atacaaaagg aagaggtaaa tgcaattgta gagataatgc atgaatttga tttaatatta
    18661 gatagaatag atacattatc aacagcttac ttaagaatgc ttaaaaattt agattacaat
    18721 gatataatgg taaccaatat aagtgacaat agcacaatta taaatatata taagaaagat
    18781 actttattta ttagtgataa tatacctata aagataactg ctaaagatat ggacatgcaa
    18841 ttattaaatg tagtagaaga atcaaaaggt cttatgaatt actattcgtc aagaaacttt
    18901 ggtaaggtta cggatactat attaatatta ggaaaaaggt attccaataa attaatttat
    18961 gaaactttta gctctgcatt ttcaagctat gtttctcaag gtcttactga gataatggat
    19021 atatctgata tgataactgg agatatagat gaagatgata taaattcaat tgtagagctt
    19081 ttaggaagta tgatagaacc taaagggaaa agagattttg aaaatataaa ctttttacca
    19141 atggatatac ttagaaaaca actaaaagct aaacagatga aaaacatact gaaaattgtt
    19201 ccaatagtta ttttgattct tgctatacct tatttatgtt taattttttc aaataattta
    19261 gcaaataaag aacttgaaaa tgttaatggt gaaataagaa atattgaatc tgcatattat
    19321 cagattggga atatagaaaa aatgatacag agtaaaagcg aagaaataaa ggtatatgat
    19381 atgttaattg gaaaaaaggt aaagtgggaa aatttattgg atgatataga taaaaatact
    19441 ccaagtaaag ttgaactaac aagtttatca acgacttatc aaaatcttgg aactaataat
    19501 aaagctgata attcaaaaag taataagact acagcagata aatcaagtaa caatgaaaca
    19561 aaatcaaata cgaaaagtga tagtaatgga gataaaacaa ataatgattt aaatgaagat
    19621 aagactacaa atgtgtcaga agcattaact ggaaatagtg agactaatag taataacaca
    19681 gataaagatg ataaaactcc attgtatgat aagataccaa atgttataaa tatagaagga
    19741 aatgctaaag attcctctta tgtaggtcaa tttttatata aattaaaggg attgtactat
    19801 ttcgaggatg ttaaattaca tagcataaaa agtaatgcag aagattctac ctatacattt
    19861 agtatgacat tatacctgaa agaaggtgta ttaactaatg aataaagata tatttaaaaa
    19921 agatataaaa gatatattta aaatggaagt tagtctaaaa gggattctta taaaggttat
    19981 aattttatta gctattttat catatggtta ttttggactt taccctcaat atcagaaata
    20041 tattgctaca aaaaattctg taaaacaagc tactgaaaaa ttaaatatat ataaagagaa
    20101 aactgcactt atgccaaaat atgaaaaaca acttgaagaa gttgaaagtg aattaaaaga
    20161 aaaaagcaaa aatcttttat atgatatgca agatggtctt tttttaatag gtttagataa
    20221 aaaaatgaag gcttataata tagacttaac tagttatgac gttggaaatg ttagagaata
    20281 cgaacatttt tatggtgtat caacaacaat aagtgttaga ggtgattata gaaatgtcaa
    20341 aaaattaatg gcttatttag aagaacaaaa aaatgttact caaatactag attatacaat
    20401 gacttcatat atagaaccag aaaaagaaga ggaagaaaca acaaacaata aggatttaat
    20461 taaaaatgtg aacataaata aagatacaaa aatatactgg gtggaaggcg aaaataagtg
    20521 gcatttatat aaggattgtc cattgataaa agagggagag attctaaaaa atgacaaact
    20581 tttagatagg tctactagtt atctatgtaa tacttgtgta cttagagctg caacagaaaa
    20641 agaagcaaaa aaagaagtcg tagctccaaa ggcagaaggt gtagtagatg ctacatttaa
    20701 atttataatg tacactaaag agaatactac aatgatactt gatacagata atccatcttc
    20761 atggagaccg ggaaaatata atccatttaa gtctagtgcg aaataaagct aagtaagctt
    20821 taaagatagg tgatgagttt gtacaaaaat aatgaaaaag gtcttacctt attagaagta
    20881 ataatagctg tttttatgat gacaatagtt ttaagcattt cttataaagt gttcaatggt
    20941 ataacatcag cagtaaaaag gcaacagatt attacagatg ctcaagtaaa tattaatcta
    21001 attaataagt atctaaatag agatttggaa aactgtaaag agctaactaa aactggttca
    21061 ggtaataatt atgagtacaa tatagagatg ccagataatg ttgtaaaata tgaagtttct
    21121 atagaaacta aaaaacatac agaggtatat tctgtaacga gaatccaaaa aaatacaatt
    21181 gatacagaaa atgaagttag agaagagata atctataatc aaccactagt tcaaaacaat
    21241 aaagaaatga aggaaacacc atttaaaata gaaaagcaaa ctggtaaatc tatttatact
    21301 gtaagtatat actataatga atcagtgcaa gaaaatcata aaaatagcaa tttaaataac
    21361 aaaacttata catttgatgt tatgtcaaga atagggtaat aagaatttat taaaaataag
    21421 taggttgata aaatatgagt aaaaagaata gtgaaagagg attttcctta attgaagtgt
    21481 tagtagctat ggctattatg ggaattgtac tatttgcatt ttttaatatt atcaatacta
    21541 ataacaaagc aaacactaaa aatgacacag atataacttc cttaaactat gtccaaagtg
    21601 aaatagaaaa tctaagagaa aagataaaaa gtggagaatt tgattttgat agtttagata
    21661 aactagaaga tggaactgtc gtatatgaaa aattaataga taaatcaaaa aaagttgtat
    21721 atgataaggt tcttagtaag ggtgacgtga gcttatatga tactccatat gaaaagatta
    21781 caacaataaa agatgaagat ggtaatttaa tcggcaaaga aaatattaca aataagataa
    21841 agactattgt agaagataaa agtggtcaga tatataaaat agctgtaact ggaaaaagca
    21901 tgaatgacta ttcaagtaaa aaagaggtta aaattgtaac tgaaatattt aaagataaat
    21961 aattttactt gggggtgaga ttttgaggaa gtggaataaa tttaaaagtg aaagaggggc
    22021 agctttggtt ttagttttgg ttgtagtagc attgctttct attgttggat tgatattttc
    22081 aaatcagata gccaatagaa taaaatctac aaagactact aatgagggta tacaagcaaa
    22141 gtatttagca gagacatgtg tagaaaatag tattgataaa gcatatgaaa aattgtatga
    22201 tgaacttgag aaaatggata atgagtttaa aagtgaaaat caagaaaaat ccatatctag
    22261 gtcaaaatta agaaatatat cagatgaaga ttttaataat caagatgagg aaaatattga
    22321 agcagaaaga ctaggatata tgaataatat aaatttctat ttaaataaag cttcttcaga
    22381 attggaaaaa gctagtatgg aattgaaaaa gttatatgat ttagatatgt tggactatag
    22441 agacattgaa tatgtagatg caaatattat ttcacataga gattctatct tagaaatttg
    22501 caagaattat acttctggag atattagtaa aataaatgaa catattctga aagaagatat
    22561 agactcaact acattgattg aagcaaagtt agtaaataat gatattcttt taaaaatgtt
    22621 tttagaagaa aataaaattg aaaatgaaca tttaaattca gccttttcac atacttataa
    22681 ggcgttagat aacatttctt tggcaatgca aaatatgatt gagtatagac atacatttca
    22741 tatagatgag ccaaaagtag aagtatctaa tgggattcca aattctcaag aatattatga
    22801 actaatacaa aatcctataa taaatagtat ggagtatagc tggaatagta aatgggatac
    22861 attagaaaac ctattggaaa ttttaccaaa tcaaactcaa ggttttaatt ctttaagagt
    22921 tcatttgaga aataatgtaa gaaaatttga gaagttaagc gataatatat ctagtggtaa
    22981 aaaaaataca gcaaaaaatt tcttaaagta taaagagcta ttgtatgaaa tatcagacca
    23041 atgtaatcaa ctaaaaagta tgtcttatga gaaaatacct gttaaatatg ataacatggc
    23101 tttgataaca acttttgact atatacaaaa tgagctttta gcagagataa aatgtagatt
    23161 aaaagaatta aaaccccaag aagttgataa aacagaaggt attagtataa aaataccttt
    23221 ttataaggct gactacgaca tgacaaaaga aggatggcct aaattaaaag aaaatggttc
    23281 aggggcggag ttaagcttaa tggtaactgg tgataaagat ggaataaaag aagtagaagt
    23341 tacagatagt aaaaaaaata ttattggttt aggtgttgaa gaaaattcaa attcaaagta
    23401 taaggttgag gccattgtaa actttaatct aaatatagat acaaatgtag ttggaaatta
    23461 taatataaaa gataaaattt tgattaatca tgatataagt tcatacaaaa aagtaaacta
    23521 aatataagga gtaacttagt ataaataaat aataaaataa ttttattatt tactactaaa
    23581 aaaataagta aactaataga agatatggag agtaaatatt atgtatattt atgatttatt
    23641 agagcaaggt ataagactaa acgcatcaga tatacacata acagttggaa caaatcctgt
    23701 agctagagta aaaggtggat ttgtaaaact aagtgaacaa atattgacat cagaagtaac
    23761 aatgcaaatg gcaaaagaca tagcagggga atctatgttt aaagtaatag aagaacatgg
    23821 agaagctgat ttttcagctt ctctaaaaac aggagaaaga tttagggtaa atgcatatag
    23881 gcaaaaagga aattacgcaa tagcaataag gactataaca gcagaaattc caacatttga
    23941 aaaattaggt cttccagaaa atataaaatc ttttactgaa aagcataaag gattagtctt
    24001 agttacaggt ccaacaggaa gtggtaagag tacaacactt gcaagtatga taaacataat
    24061 aaatgaaaaa caacaaaaac atataattac acttgaagac ccgatagaat atgtacatca
    24121 ccataaacaa agtttagtaa atcaaagaga agtaggaact gatacagaaa gttttcattc
    24181 agcccttaga gctattttgc gtcaggaccc agatgtgata ctaataggag agatgagaga
    24241 ccctgaaact gtttcaatag cattaacagc agcagaaaca ggtcatttag tattttcaac
    24301 actacataca gtaggggcag ctaagacaat tgatagaata gtagacatgt ttcaaccatc
    24361 ccaacaacaa caaataaaaa cacagttatc tacagtttgt gaaggtgtgg tatcacagca
    24421 gttacttcct acagcagatg gaaaaggcag aatagcagca attgaattga tgtttgctac
    24481 tccagcaata aaaaacctga taagggaagg caaaacatat caaattccaa acatgataca
    24541 aactggagtt aaatcaggta tgaaaactat ggaccaagat ttaatggaac tttataaaaa
    24601 tggaaagatt acaaaagata tggcacttag taggtgtact gaccaagagt ttatgacaag
    24661 aatgataggt ggagtaaact acaatggata ttataatcgt taatctatat gtatttatag
    24721 ttggtatagt gtttggaagt ttctttaatg tctgtatttt taggattcca gaaggtatat
    24781 ctatttctag acctccctcc cattgtatga gttgtaatac tagactaaag ccaatagact
    24841 taataccagt gctaagctat ttaatgagtg gcagaaagtg tagatattgt tatgagaaaa
    24901 tttctagtag atatgctata gtggaattaa ttacaggaat tttattttta acagtattta
    24961 tgttatatgg aatatctaca tcaactattt attatttggt tcttgtgtct ttactgatta
    25021 taattacatt tatagatatt gaccacttta taataccaga taaaatactg atttttggag
    25081 ctatattttc tataattttt aaccttttat ttaaagaaat tccagttaag gattcaatta
    25141 taggtgtttt tatttgtgga ggaagtgtct ggattatagt tcttttgatt gaatttgtaa
    25201 taaaaaaaga gtgtatgggt ggaggagata tcaaactatt tgcaatgcta ggtctttaca
    25261 tgggtgttaa aaatggtctt ttgacagctt tactaagcgt ttatgtggga gcagtctatg
    25321 gaatctgtgt aataattaca agcagaataa aaggaaagga atataattct gttataccat
    25381 atggaccttt tatatctatt ggagcattaa taacaatctt atgcggaaaa caattacttg
    25441 aattttattt ttctatgatt atatagatga tagtcaagtt aaataaagtt ttaatttaaa
    25501 aaaatgaatt aaagtattta ataaggacaa tattattatt aaccctatta ctaaagtgct
    25561 acaaactatg gagtatataa taataatttc cttatttttt gcacatttga taaataaatc
    25621 tctatgtgtt ttttgtgata gagaaaagaa aaatttatat aatacgtttt ttatttacat
    25681 aataactgta gttttatctg tattaatata tctaaaattt gggattagtg tagaatgtgt
    25741 acaatatatg gttttaattc catttttaat aattatatcc attgtagatt actacacaat
    25801 atatgtatat gatataacaa tagttagtgg tataatagtt caaggtataa tttttttggc
    25861 ctttttcaat atagctcaat ttgattatat aaatcatata tttggactat tgataggctt
    25921 tattatacct tatattattg ctaaactaac aaaaggatta ggctatggag atgtaggtct
    25981 ctatagctta tgttgctttg cattgggaaa taattatggg ttttatgtga tagttttatc
    26041 atttatttta gcgtttttgt tttttattat tttatacata actaaaatat ttaaagatgt
    26101 agatataaga aagcaaattc cttttacacc ttttatttcc ttgtcaacaa ttttaataat
    26161 atttacagag tataatatat ttaattttta ttataatttg ttatacaaat ttatataaca
    26221 ggaggtaaga aatgtacgta gtagtagggt taggtaatcc aggaaagaag tatgagaaga
    26281 ctagacataa cgttggattt gatgttatag atattcttgc taaagaatac aatattagtg
    26341 ttactaaaat aaagcataaa gctttgattg gtgaagggag agtaggtact gaaaaagtat
    26401 tattagttaa accacaaact tatatgaatt taagtggaga aacattaata gatatatata
    26461 aatattataa ggttgattta agtaatatag tagtagtata tgatgatata gatttagaag
    26521 ttggaaagat acgtataaga aaaaaaggaa gtggaggaac tcataatggc atgaaatcta
    26581 taactaagtg cctaggttca aatgactttc caagagtgag agtaggtgta tctaaaccag
    26641 aagctggtca agacttagca gactttgtac tttctagatt tagaaaagaa gaatctgata
    26701 acataaatga agcattagaa aaagcggcag atgctattga ctcaataata agagaaaata
    26761 tagatatgtc tatgaacaaa tataatggat agtgagaggg agatttaaat gaatgatgtt
    26821 tttttatatc ctttgcaaaa ttcaaaagag tataaagata taataaactg tataaaaaat
    26881 acaaaaggct cattattggt taatggactt ttaccagtac aaaaacctca tatatcgtat
    26941 tctatattta atgatttatc acgacaaatg atttttataa cgagtagtga tttagaagca
    27001 aagaaagtat acgaagattt aagtttttat atggaagata aagttgaata tttaggtttt
    27061 caagatattt atttttatca cttggatgca aaggacagaa acgaagaagc aaagaaatta
    27121 aaagtattgt taaaactagc aaataaagaa aaaatcattt tagtaacttc tattgaggca
    27181 gttttaagaa aatacatacc aaaacaagtt ttgctcgata gtgtatctca ttataaggtg
    27241 ggagatagct tagacttaga aaagttaact gagaaattag tttcattggg atatgaaaga
    27301 gtttcaaaaa tagaggggtt tggtcaattt agtataagag gtggaattat tgatgtattt
    27361 tcacttgaat atacaaatcc aattcgtatg gaattatttg atgatgagat agattccata
    27421 cgaacttttg acgtatattc acaaaaatca attgataaat tgcaacaatt ctctataact
    27481 ccatctaggg aatttattta tcccgaaaaa actacagatg cattagttaa attaaaaaaa
    27541 gaaacaacta aaaatactga tgaaggtaca tttcaaaata tagattatat atcaagtaag
    27601 acttattttg aaggtgcaga gaactatata gattatattt atccagaaga aaacaaaagt
    27661 atatttactt atttagcaga tgatgcaata gtatttatca atgatataac gagacttaaa
    27721 gaaagatgcg aaaattatat aaatgagttt agagaaaatt ataaattaaa tcttgaaaga
    27781 ggtctggcta ttaaaaacca aggtaaattg ttatatcact atacagattt agagtactta
    27841 gtaaaagaca agtctgtggt attaaactcg ctattgccaa agtctataaa taacttttct
    27901 ataaaatcta taataaattt tgaaagtaga gaagttccta catttaatgg aaaggtagat
    27961 ttgcttgtag aagaattaaa tcggctgaaa tataatggat acaaaataat tttagcaaca
    28021 aataccttag aaagagcaaa taaattagga aaagatttat tggacaaagg cttagaaacg
    28081 accatttcta aagatagaga tattgaaatt aaatcttcac aagttataat tgtacctgca
    28141 cacataaaca gtggttttca gtataaatct ataaagtttg ttgttataac agataacgaa
    28201 atgataggtg tttataaaag agcgtctaag actagcaata aaaaagttaa aaaaggaaag
    28261 aaaatagaat cctttttaga tttaagtgtt ggagactatg tagttcatga aaatagtggt
    28321 gtaggtagat acacagggat tgaacaaatt acagtcaatg ctattaaaaa agattatatg
    28381 aaaatagtat atcaaggtgg agataatcta tatgtgccaa tagaccagat ggacaaagta
    28441 caaaaatata ttggtgcaga agtagaaaaa gttaagttaa ataagcttgg aacaaatgag
    28501 tggacgaaag caaaagcaaa agttaaaaaa gaaattgaag atatgacaaa agacttgatt
    28561 gagctttatg caaaacgtga aaaaatacaa gggtataagt tttcaaaaga tacaccttgg
    28621 caggcagagt ttgaatcgtt gtttccatat caagaaacag aagaccaatt aaaggctata
    28681 gaagaaacta aaaaagatat ggaatcaagt aaggtaatgg ataggctagt atgtggagat
    28741 gttggttatg gaaaaacaga ggtagctatt agaagtattt ttaaagcttg tatggaccaa
    28801 aaacaagttg ctgtattggt tccaacaaca atattggccc aacaacatta taatacattc
    28861 aaagagaggt ttgaaaatta tcctttgaga gtagaagttt taagtaggtt taaaacacca
    28921 aaacaacaaa aacagattat tgaagatgct aaaaaaggat tagtagatat tttaattgga
    28981 acacatagaa taatatccaa agatataaat ttaccaaact taggtcttgt agtcattgat
    29041 gaagaacaaa gatttggagt aaagcataaa gaagctttaa agaaaattaa atctacagta
    29101 gatgtactta cactttcagc aactcctata ccaagaacat tacatatgtc tttaagtgga
    29161 ataagagaca tgagtgttat agaagaacct ccacaagaaa gacatcctgt aataacatat
    29221 gttacagaga gtaaggaaag cgtaattcaa gatgagatag agagagaatt aagtagaggt
    29281 ggacaggtat tttttgtata caatagagtt gaacacattg aagaaatggc aagtatgatt
    29341 caaaaactcg taccagatgc tagagtggct gttgcacatg gaagaatgac ctctaaatca
    29401 ttagaaaata taatcctagg atttttaaat aaagattatg atgtactagt ctgtactaca
    29461 ataattgaaa ctggtatgga catatcaaat gcaaatacaa tgattatata tgatgcagat
    29521 aaaatgggat tggctcaact ttaccaattg agaggtaggg ttggaagaag ttcaagacaa
    29581 ggatatgcat atcttttgta tgaaaaagat aaaactttaa gtgagatagc ggagaagaga
    29641 ttaaaagcta tcagagaatt tacagagttt ggttctggtt ttaaaatagc aatgagagac
    29701 cttgaaataa gaggagcagg aaatatttta ggttctcaac aacatggtca tatggcagtt
    29761 ataggttatg atttgtacgt taaaatgctt aatgatgcaa ttaaaaaagt caaaggtgaa
    29821 cctattgtgg aggaaattga tgttgaaata gatttaagtg taaatgcata cattccagat
    29881 aattatatca aagatgagtt gattaaaatt gaaatgtata agaaaatagc ttctattgaa
    29941 aacaaagaag acatgttgga tatacaagaa gaacttgagg atagattctc tgatatacct
    30001 aaaccagtcc agacacttct tacaatagct tatataaaat cgttatgtaa gatattaaaa
    30061 atagaaaaga taagacaatt aaaagatgaa atattattag ttccaataac aaagtataga
    30121 actaaacaga aaataggtta taatatagtt acagaattag aagaattatt agaaaagatg
    30181 tgcaaggtaa aataacttgt aaatttaaga agggatgttt taaatttgaa aaaagtaata
    30241 acattggtaa tagctatgat tttggtagta tctgtgactg cttgcagttc tagtaaagga
    30301 gaaactgtgg caacagttga aggtacaaag atatcatcag atgaatttaa aaaaactata
    30361 gctctttata aagattctat ggaacaaact tatggaaaag atatatggga taaggaagtt
    30421 gaaaagggtg taaaatacaa ggacaagttt aaagatttaa ttcttgacca acttataact
    30481 acagaagtaa tatattctca agctaaaaaa gataacttac ttcctaaaaa agaggatgtt
    30541 gaaaaaagct ttaaagaatt aaaagacgct atgggtaaag atgaaaaata taaagaacaa
    30601 cttaagaaat taggtataga tgatgagttt ttaagagacc aacaagagaa agatttagca
    30661 atgcaaaact atcaaagtaa ctttgctaaa aagactaaaa tatcagatga agaaatgaaa
    30721 aaatactatg atacacataa agatgaattt aaaaaagatg aggtagaagc ttctcatatt
    30781 ttattgaaaa cagtagatga taataacaag ccactttcag ataaggaaaa agctgaagct
    30841 aagaaaaagg ctgaagaggc tcttaaagaa gttaaatcag gtgaggattt tgcaaaagta
    30901 gcaaaaaaat attcacaaga tacttcagca agtgatggtg gtaagttagg attcttttca
    30961 aggggtcaaa tggttgctga atttgaagat gctgcttttt ctatgaaaaa gggtgaagta
    31021 tctgatttag ttgaaactca atatggatac cacataatta aagtgacaga cagaataaat
    31081 gagcaaacat catttgaaga tgctaaagaa actataaaag accaactgtt gaaaaataaa
    31141 tatcaagaac aaatagaaaa attaactaaa gaagctaagg tagaaaaaga tgaaaaagta
    31201 ataaataaaa tcacaatcta agacttaaaa aaagagtaat atgcttaaaa aagctatatc
    31261 tggataagat atagcttttt ttgtatattt ttcttaacaa tggtaaaaat aaacttaaag
    31321 taatttatgg agggattaaa aaatatgaaa gcaacaggta tagttagaag aatagatgat
    31381 cttggaaggg tagttatccc aaaagagata agaaagacat taagaattag agaaggagac
    31441 cctttagaga tattcacagc aaaagatgga gaagtcatac ttaaaaaata ctctccaata
    31501 ggtgaattaa atgaattttc gcaagaatat gctgaaacat tagctgaagt actaggatat
    31561 ggagtgttga taacagactt agattctata atagctgtgt ctaagcttcc taaaaaagat
    31621 tataaagaaa aaagtataag tgatgagtta gaagcaataa taagtgaaag aacagtaaaa
    31681 tattcaaaag ataaaaacat tttaccatta ttcaaggaag accaaaaaga atatactagt
    31741 caagttatca tgcctataat tagtgcatct ggtgactgta taggttcaat tgtagttgtt
    31801 tcaaaggata aagatacaat tgcagaaagt gaagaaaaat ctttaaaaat tgctgctaac
    31861 tttttaggaa aacaagttca ataattttat cataaagacc ttttacatga aggtcttttg
    31921 aagtttttgt gtaaaatatt gaatatagca ttaaaaatat ggtatattaa agtttgttat
    31981 ataaacagta tggaggttat gttatgaaca ataacaaaaa taaagactcc tttctaaaag
    32041 gagcccttat acttgggcta gcaggtgtaa tcgtaaaaat aatgggtgga ttttttagga
    32101 ttccccttgg aaacatgatt ggttcagaag gaatgggata ctatcaggct gcttatcctg
    32161 tgtatactct atttttaaca ttagctactg ctggatttcc aacagctgtg gcaaaattgg
    32221 tttcggaaaa agttgcaatt ggaaatttta aaggtgcaaa taagatattt aaggtatcac
    32281 atacagtttt attcattacc ggtataatat cattttgtat attatttttt ggagccgact
    32341 acatagtaac aaacgtaatg aaaaatccag gtgcgttata ctctatgaaa gccattgctc
    32401 cagcattact atttgttcct gcaatgtctg catatagagg atattttcaa gggagacaag
    32461 atatgactaa gattgctgtg tctcaagttg cagagcaatt ttttagagtt gtattaggtc
    32521 ttactttggc ctacttgctt atgaagagtc taggtcagac ttttggtgca gcaggtgcta
    32581 tatcaggggc tacaataggt tcaatagcat ctatgcttta tttggtgttt gcttatatgc
    32641 tagggagaaa agagcgtaga gcagagatag atgcaagcca aagatttaaa gatgaaagag
    32701 tgtcttatat ttttaaaaag cttttaactg ttgccattcc tataacaata ggagcatcag
    32761 taatgccact tgtaaatatg atagataatg ttattgtaat aagaagactt atggaagcag
    32821 gctttacata caaagtggca aattcaatgt ttggtcaatt aacaggtatg gcaatggcca
    32881 caataaattt accagcagtc ataacaactg caatgagtat gagcttagtt ccagcaattt
    32941 ctaaagcata tgcattagga aataagtcaa aagctagaaa agatactaag agtgcagtta
    33001 aggtaacttt acttatagta ttgccttgtg cgtttggtat ggcttcttta gcaataccaa
    33061 taatgggtct tttatttcca catgaaccat cttctgtagg aactattttg tttactctta
    33121 ctccatgtgt attattttta ggattaatac agacattgac tggtataatt cagggaatgg
    33181 gaaaaccaat agtacctgta attgctttat gtgttggtat gctatgtaaa attgtaataa
    33241 gttatacttt aacaggtata ccagatatta atgtactggg ttcagcattt ggtacagtta
    33301 cagcatactt tgtggcagcc atgattaact tactatatgt taaaaaacac atgaatgtaa
    33361 acttttctaa aaaagaattt ataataaagc catttataac agttatgaca atgtttataa
    33421 tggttaagtt gagttatggt gctttagttg gattcttagg aaacagcata tctacaataa
    33481 ttgcaatatg tgttggtgga attgtatatg tagtagtaat acttggaata ggtggaatta
    33541 aaaaagaaga gattctaact atgccaaaag gtgataaatt atataaattg ttaaaaaaag
    33601 ttaaacttat aagataatat aggaatatat atacaaataa aggaaatact atacaaatag
    33661 taaatctcct tatttgtata ataatataaa aataaagtga aatttgctaa taggagtgat
    33721 gttatgggta agataagtat tgtaggtctt ggaccaggag actattctct tataagccaa
    33781 ggtgtgttag aatctttaat aaatagccct aaagtttttt ttagaacaga gaaacatcca
    33841 ataattgata aacttaaaga aactatacaa tacacttctt tagactactt ttatgaaaaa
    33901 gaggaaaatt ttgataatgt ttatttgaaa atatctgaat ttatcataga agaatcagca
    33961 aagggtgacc tagtgtatgc tgttcctggt catccaagag ttgcagagaa gactgtaagt
    34021 ataatagaga gattagccaa tgaaaagcac atagcagtag aaatattgcc ttctatgagc
    34081 tttgtagacg ctatgtttaa ctatttggct atagacccat ctgagggatt taaactggta
    34141 gatgcttttg aaatagaaaa ttcatatata gacttggata caagcatgat aataactcag
    34201 gtatatgata aatttatagc atctaatgta aagttaaaac ttatggaaca ttatgactat
    34261 aatcaagaag tatgtatagt aaatggagca ggtgtcaaag atttagaaag taaaaagttt
    34321 ataaagcttt gtgaattaga cagaaatgaa aacttttttg actatttaac tagcttatat
    34381 atacccaaaa gttctaaaaa gatgtataat acagtacatg acttagaaat aatagttaat
    34441 acacttagaa gtccatctgg atgtgaatgg gataaaaaac aaacacatca atcattaaaa
    34501 aattctgtta tagaggaagc atatgaactt tgtaatgcaa ttgataataa tgatatagat
    34561 gaaatggtag aagagttagg tgatgttctt ttacaggtta tattccattg tcagatagga
    34621 aatgaggagg gctactttga tttaaaagaa gtagtaaatg gcatatgtaa aaaattaatt
    34681 catagacatc ctcatatatt tagtggtgca aaattagata tgtctaagtt tgagaaaact
    34741 tgggaagaat taaagaaaga agagaaaggc gaaagtacta taacagatgg ccttagaagg
    34801 ataccaaaac atttaccagc tcttatgaag gctagtaaag ttcaacataa agcagcactt
    34861 gtcggctttg attgggacaa tgtagaagat gtatttaaaa aaattgaaga agaatatcag
    34921 gaattacttg acgaacacag ggtcgggaat ataaaataca taaaggaaga gctaggcgat
    34981 ttattatttt cgatagtaaa tctagctaga tttttagaca tagactcagg agaagccttg
    35041 aactgtacta cagaaaaatt cataaataga tttgacttta tagaaaaaag tgcaatgaac
    35101 ttgaataaaa aattagaaga catgacactt gaagagatgg atgagttttg gaatcaggct
    35161 aaaaaacaat aaaattgtgg aaggaaattt gtcaaaaatc tagaactttt atcacaaaga
    35221 tagaagtcta tattataaac atttttagga ggtaaatatc gtgaataaag ctgaattagt
    35281 atcaaagatg gcagaaaaaa gtggattaac aaagaaggaa gcagaagctg cgttaaacgc
    35341 atttatgagt tctgttcaag atgcactagt aaataatgaa aaagttcaat tagttggatt
    35401 tggaacattt gagacaagag aaagagctgc tagacaagga agaaatccaa gagatccaga
    35461 gcaagttata gatataccag cttctaaagc accagttttc aaagctggaa aaggattaaa
    35521 ggatataata aatggataat tagaaatctt gtgaaaaagt gtggataaat ttgccacact
    35581 ttttctatag aaccaaggaa ggtgataatt atgagattag ataaattttt aaaagtatct
    35641 aggattataa aaagaagaac tgttgctaaa gatgcatgtg ataaagggat tgtcactata
    35701 aatggaaaag ttgctaaatc ttcttcagag gtgaatattg gagatacatt agaaatacaa
    35761 tttggagaga agaagatgaa gtttaagata aatgaagtaa aagagcatgt attaaaaaat
    35821 gatgcaaaag aaatgtacga aattttagaa taaaattaat agtcctctag tatattaata
    35881 gtagtattat tgaaatattt actgggggga tattatggaa cagaacataa ctttgaaaga
    35941 tagatcaaag ctggttattt cgggagtcga gcacatatac tcttttaatg acaaaagagt
    36001 agagctcaaa acttctgtag gcgagatggt aattgaaggc gaaaacctag atatgagcaa
    36061 gctaagtata gacgaaaata taattagcat agatggaact ataaattcta tggtatatgc
    36121 aaagcctaaa aagccacaag agagtttctt gaaaaaggta tttagataga tgataccttt
    36181 tactcaagat gtaagcattt tttatgctac tatttatggt ggaatactca taggggtgtt
    36241 gtttgatttt tatagagggc taagagggaa ttttaaattc atcaattatt ttgctataat
    36301 ctttgatgta ttgttttggt ttctagctac ggttataata ttcgtaacaa taaaccttac
    36361 agaatttttt gatttaagat attatcattt tgtggctttg tttattggtt ttattttata
    36421 ctataacacc ataagtaaaa ttgttttatc aattatcaat aaaataattc gttttgtgag
    36481 aaattctttt aaaaaagtta cacattacat agtcagtttt ttaaataatt tgtattacgt
    36541 tataatctat tcactacacc tactatttga tattatattt tatataccaa acatatttat
    36601 agcaacaaga aaaagtataa aaagaaaatc aaataaaaaa ttaaagaaca agaaaaaatc
    36661 aaaacctaag aagaaaaaaa ataaaactaa aaaaagggtg taggaaaaat gaacttaaga
    36721 aaaaaatttt caggacagat tattgttgtc agtttatttt taggaatatc aatttttagt
    36781 atgatgacag gatttgtatt tgagtataca aaagctaagg aatataaaaa agaaatagca
    36841 agtctaaata aacaattaaa aaagactgaa atacaaataa acagcttaaa gaaagatgaa
    36901 aaatcatatg aaggagactt agaggatata gcaagaaaaa gattgaatat ggtaaaacca
    36961 aatgaaacag tttatgtaga cataaatcgt tgattaaaac ttgctaataa gtttacagat
    37021 gtattgattt taaaatttaa gatttaataa aaaagtcatt cagttaagga tggctttttg
    37081 tttttattga ggagggaatt tcataatgaa agtaggagct atagatattg ggacaaactc
    37141 aatgagatta ttgatagcag attatagaga tgggaatttt ataaacagaa aaaaatttgt
    37201 taatacaact agaattggtt atggtgtgga tagtaaagga tatataaaag aagatgctat
    37261 caaaagaaat ataaaagcat taaaagaatt ttctgataag tgtaatgatg aaaagtgtga
    37321 aaaaatttat tgtatgggga catctgctct tagagacagt aaaaatggag attactttgt
    37381 taaattagct aaagaagaaa ctggaataga tgttgatata gttacgggaa tagaagaatc
    37441 aaatttgggc tttaagggtg ttttagaagg aatacaaggt gatgatacta ttattgttat
    37501 agatattgga ggaggttcaa cagaatttat tgtaggtgat tcagaaggaa ttaaatttaa
    37561 taaaagtgag aatgtaggtg ctcttaggat gacagagagg tttttgaagc acgatattat
    37621 tgaagatgaa gagtttgaag ctatgagttc atttatatat gatgagattg acaaaacgtt
    37681 aaatcatata aaagacagag gattaaaaac tcttataggt ataggaggta cgattacatc
    37741 attatcagca atgaatcaag aattagagat ttactctatg gaaaaaatac ataatagtaa
    37801 aatacacaaa aaagaaatag aagaaattct acaaaaatta ataaagatga cattaagtga
    37861 taaaaagaac ttaaaagggc tacaagaaaa acgtgcggat attataactg ctggtgtaaa
    37921 aatattgaat ataataatgg aaaaattaga aatagaagaa ataaccgtaa gtgaatacga
    37981 taatttggaa ggcttaatat gtcaaaagtc aaaaaaaatg tattaatatt ttttgatttt
    38041 taaaaccgat tatgacaaaa aaaattactc ctctttgtta caattcttaa tataaacaaa
    38101 agaggaggtt tttttatgca aagaagtgta gcagttgtga agaataagac tgtaaaacca
    38161 aatgtcataa aattaataag cggatatatt gattttacta cggcggcatt cgctgtgatg
    38221 ggatttttac ttagtaggtc tattatagta gactctgtag ctcctttagg aattgccttt
    38281 tttatatgtg catcaaagat tgataagtac aagatacctg tattcatatc aacattatta
    38341 ggaatattgt tatcatctaa tgattttgga aatgtagcaa aatatgctgt gtgccttaca
    38401 atatttatgg ctatgaataa aaaattaaaa ttatcagatt caattgctaa ggttgcattt
    38461 ataggtgcaa taatactact tccaatctcc atgggacaag ctttcttttc aaataggtat
    38521 gtatatggtc ttcttatagc aggagtagaa gcaataataa tgtttatatc tatatatgtg
    38581 ttttcttatg ggataggttt aatggccaat gtcaacaata ggatgtctgt acggacagaa
    38641 gaagccatat caataagttt actgctaacc tttagcataa tgggaatagg agatatatct
    38701 ctgtttggaa tatcaataag gaccatttta tcgaccgtat tgatattgct gacctcaata
    38761 gtaggtggag ctaccatggg agcatctagt ggtgtaatta ttggtgtagc atccatttta
    38821 aataatatga catcagctgt ttatatgggg atatattctt tttcaggatt aataagtgga
    38881 gcatttaata aaataaataa atatttctgt atactaggat atattctaag ttggacaata
    38941 atatacctat atacttctgg tattacttct aatatgatgc aattaagaga tatactatta
    39001 ggttgtttga tagtactagt attgccagag cgattattta ataagattga aaaattaata
    39061 aaaagtaatg tggcttcaaa tgaaatcgta tacgattata ttatgagaag caaaaaccta
    39121 actaatagca gattgaacag tatttataaa acttatgatg accttgcaga tacatttgat
    39181 aagataagag aaaaagacaa agtgctagac caaagagaca tagctaatgt aatagatatg
    39241 attcataatg atgaatgtaa aagttgtagt atgagaagaa tgtgctggga gtcaagattt
    39301 aatcatacat acactatgat atataacatt cttgaaaaaa ttgaggagaa aggggagtta
    39361 agtctaaacg atatacctaa aaattttaga aaggaatgta tgaaaccaga gtcaatagtg
    39421 aaaatatcaa atcactatta caaaatgttt gtattagatt atgattggag tgtgaagttt
    39481 tcagaaagta gaaagttaat agcaaatcag ataagaagta tttcaaaatc cattaaatcc
    39541 ttatctcaag atttagaagg cgacataatg ctagatatag aaaaggaaaa aaatatatat
    39601 gaacagttag aaagatatga tataacagtt gataaagtaa gttacttaac aaaaagtaac
    39661 agtgagtttg aaatatcaat agaaaagaaa acttgccatg atggatgtat gtgtgaagat
    39721 aaaattgtaa atataatttc agacctagta ggggaaaata tgtctgttag aaaaatagga
    39781 tgtcactgtt taggaggtaa atgcaaggca acattcgtta aatctcaaaa atataaggct
    39841 gttacagaag tttcagcaat gtcaagggat gggcatatcc tttgtggaga caattacaca
    39901 tacatggaga taaatgatgg taaatacatg atggctataa gtgatggtat gggaaaaggt
    39961 aaaaaagcat atgaagaatc ttctgccact attgatatac tggaaaaaat gatagatgca
    40021 aaaattaaag atgagatagt cattgatact ataaataata tgttgttgtt aaaatcttca
    40081 gaagagatgt tttcaactct ggatttggga attttagatt taaaacgagg ttgtttagaa
    40141 actataaaga tgggagcgtg ttctacttat ataaaaagag aggatggaga agttgactta
    40201 atatcatcat catcattacc tgtgggtata ttatcagatg taaaaattga tagaaaaaat
    40261 gtaaaagtta aggaaggaga ctatgttata atggtttctg atggaattgt agatgcagga
    40321 agaaataaca atttaggaga caattggtta atatacttct taaagaatat agagactaca
    40381 aatccaaagg aaatttcaaa tttaatattg gatagagcat tagagttaca agcgttacaa
    40441 atcgaagatg acatgactgt tttagttacg aaaatatgta caaaataagg tttacaagat
    40501 tatgtgtata agatgtggat aacttgtgta taatatgtta atagactttt gaaaaaagta
    40561 tagttattaa caaaaataac atataaatca acaaaatttg ttgataagta tggcagaaat
    40621 gttaataaaa tgtaaaaaaa gaaaaaacaa aagctattat caaaagagat tcatgttgaa
    40681 ataactaaat ttctaagtaa agaaaacctg tgaatatgtt gattattatg tggataacta
    40741 ttaattcgat atttatttat atagtatgaa taattgaata atatatctaa aaaaagccat
    40801 cctattatta agtaaactta ttgaaaggat ggctttttat gaatagaaaa ttgtgcaaag
    40861 caataatacc tttatttatt tctatttttt gcatgttaat aataagtata aaagctgacc
    40921 aaggatatca aaagaataat gacagagcaa ttactgtcat gaataataat gatactgaag
    40981 atgatgctga aaaaaaacaa gaaatagact atgaaaaaac atgttggaat ttgcaatctt
    41041 tgtttaaaag tgatgaccaa tggaaaaaag aacttaagag ttttgataaa gatactaaag
    41101 agttgaaaaa ttacataggt aaagtaacaa aatcgccaac acatttgtct tttgccttaa
    41161 acattaaaga aaaacttgat ataagactaa ataaactttc tgcctatgta aaattaaaac
    41221 aagatactaa taaaaattcg tataagtact tagatatgaa tgatagtatg agtaaatcct
    41281 atgggaatta tatggggatt tgttcagatt tagagttaga aatactaaaa ttatcaaaca
    41341 aagattacaa gaaaattatt tcaaataaaa atataaatag gaaatatggt gcatatttaa
    41401 gagatataag aaggaataaa gtacattatt tggatgataa atcagaagat attctaagca
    41461 aagtatcttc aataagtagt cttccaagcc aagtgtatga attatttaga aatatggata
    41521 gaaaaactaa tttgacacca gctcaatatg cgagtgaatt agagtcatca gatagagaaa
    41581 atagaaaaaa agcttatcaa gatgaactta taacttacaa tgataatata aatacaattg
    41641 caggtttaat tactggtcaa gttaaaaaga atatttttta ttctcaggaa agaggttatg
    41701 agtcttcttt agaaatgtac ctggaatctg ataatgtaga tgaaaaagta tacaataatt
    41761 tgataaggac tgtaaatagt aatatggaca gtctacacaa atatatagat ttaagaaaaa
    41821 aagtattaaa attagataag gttcattcat atgatatgtt tgtgcctatg gttaaggctg
    41881 ttgataataa tattaattat gaaaaggcac aaagtatagt atattcagcg ttgtcacctt
    41941 taggaaaaga atacaatgat gtagtatata aagctttcaa tgaaaaatgg atagatgttt
    42001 attcacatga aaataaagta agtggtggat actgcttatc tgtttatgaa aatcatccat
    42061 atatactttt aaattataat aattcattag gttcagtttc gactttatca catgagcttg
    42121 gacatgctgt atatgaatat ttaagttcta agaatcagaa ttattttaat tcaaatccgt
    42181 ctatatttac tcacgaagtt gcatctacaa cgaatgaagc attactttac gagatgctta
    42241 taaaagaagc taaaggcaat gatgaaaaag cttattatat aacacagtat ttagacttga
    42301 taaaagatac tctttataca caaactatgt atgctgaatt tgaaaaaact atacacgagt
    42361 tagtagaaaa aggaaaaagt gtaaacgcat tagtattaaa tgatgtttgg ggacaacttc
    42421 ttaaaaaata ttatggacaa agctatgaat tagaccaatt atctaaggtc ggatgggcta
    42481 gaatcccaca tttttataat agtttctatg tctataaata tgctacaggt tgtagtgcag
    42541 gagtaagttt tgcccaagac atcctaaaga atggttcaga taactatatg agctttctta
    42601 aaaagggaag ttcagattat ccaataaact tgttaaaatc aagtggcata aatttaacta
    42661 atacaaaacc catagaaaat acaatcaaaa aatttgatag tttagtagca gaacttgaaa
    42721 agctattata aacattattg taatccacat ggtaaatata tcgaattaat gataaaatat
    42781 actaaaagtt taaagtccta tcaagatagg actttttatt tattaattag tgggagatga
    42841 ttctatgcaa gttaaagtta aaaaggctgt aataccagca gcaggtttag gaactagatt
    42901 tttgccagct acaaaggcac aaccaaaaga gatgttacca atagttgata agccaactct
    42961 acaatacata atagaagaag ctgtggcctc aggaatagag gaaatactaa taataacagg
    43021 tagaaataaa aaatctattg aagaccactt tgataaatct gtagaattgg aacttgattt
    43081 agagaaaaaa ggtaagaaag aattgttgga ggtagtacaa aacatatcta acatgattaa
    43141 catacattat ataagacaaa aagagccaaa agggttggga gatgcaattt attgtgcgag
    43201 gcattttata ggtgatgagc cgtttgcagt aatgttaggg gatgatattg tagataatga
    43261 tgtaccttgt ctaaaacaac taacagatgc atatgaggaa tatagaacta caatattggg
    43321 tgttcaaaag gtaaatcaag aagatactaa taaatatggg ataatagaag caaaaaatat
    43381 agaaggtaga gtttacaagg taaaagacat ggtagaaaag ccagagtcag gaaaagctcc
    43441 ttcaaatatt gctattttag gtagatatat aataactcct gaaatctttg atattcttaa
    43501 ggatttacca ccaggtaaag gtggagaggt acaacttaca gatgctttaa aaatattatc
    43561 taagaaagaa gctatgtatg catacaattt tgaaggcaag agatatgatg ttggggacaa
    43621 gctaggattt ttagaagcta ctgttgactt tgctctaaag aaagaagacc ttaaggaaga
    43681 ttttataaaa tatttgaaac atgtatgtag tgaatttgat aaaaataaca atgtgttata
    43741 caataataca gatgaaaaaa tagatttaat tgaagtcaat gaagctagag aacttgaaat
    43801 tcaaaaataa ttaatgaaag gtgatgtatg gtggcaaaat caagtcagaa aagaattaat
    43861 agaataatag ctgttgggat tgtgggaatc ctcgtaataa cgagtataac agctgttttg
    43921 agtatgattg caactatgta ataagataaa tgacagcagt tgatatacat aaatataaaa
    43981 accatatata acttgatgta gtcaaaaact tacaatattt tagttatata tggtttttag
    44041 aatttataaa tgtaaaaaaa aatttttata atagttgcat attttaaagg aaaatattat
    44101 tataagatat aaatatagtg ataaatttat aaaaacgtgc taagattgga cttgaaatac
    44161 agtaatatag taggtaataa tgttttaaat tttatttatt gaccataata tataaataat
    44221 gatattatta gacttaactg tttaaataaa acttttcttt ttcatgcctt caatttccaa
    44281 aataagtatg tatataaatg aatatataga tgaggaggat ttttttgatt ttagatgata
    44341 aagtattaga aggattaggt agtaagacct tagttgagct acgagagata gctaaagaat
    44401 taaaaataaa atcaattact acatataaga aaaatgagtt aattgagatt ataaattcca
    44461 aaagtaaaaa tgaggaaaaa gtaaacgaag agactaaaga aaaaaatgaa gatattaaag
    44521 aagacataaa gaatagaaaa gaaaatatag agcgtgaaag tatagaaaaa gaagttgaat
    44581 ataagagccc taccaaatca tacaacaaga aagaaataga tacccaaaat atttcccaaa
    44641 accaagaaca tagaaatcaa agaaatgact ataataagac aacgagtgta aatgatagta
    44701 ataagtcatc aaatatatct aatagaatgg tcagaaataa taataaaaac tattatatgc
    44761 ctaaacaagt tgatgaatct aagatagtag atgaatttaa cacatcaaaa gaggatgaag
    44821 tagtaggtgt acttgaaatt ctaccagatg gatttggttt tttaagaggt tcaaattatt
    44881 tgtctactga aggagatgtt tacgtttcac catctcaaat aagaagattt aatatgaaaa
    44941 caggagacaa gattaaggga ataactagac atccaaaaag tggagagaaa tttagagcac
    45001 ttttatatgt tcaaaaaata aatgatgaaa accctgacac agctatacaa agaaatgcgt
    45061 ttgagacatt aacaccaata tttccagaag aacgacttac actggaaact aatagaaatg
    45121 aaatagctac aagaattata gatttaattt cacctatagg aaaaggacaa agaggattga
    45181 tagtagcacc tccaaaagct gggaaaacag ttcttttaaa aagcgttgca aatagcatag
    45241 ctaaaaatca tccaaatgtt gaacttatag tactccttat agatgaaaga ccagaggaag
    45301 ttactgatat gaaagaatct attgaaggag acgttatata ttccactttt gaccaagtat
    45361 caagtcatca tgtcaaggtt gcagagatgg tgttaaatag agctcaaaga ttagttgaac
    45421 atggtaaaga tgtagtaatt ttactagata gtataactag attggcaagg gcatataatc
    45481 ttacaatatc tcctacaggt agaactttat ctggaggtat tgaccctggt gcacttcatg
    45541 gacctaaaaa gttctttggt gcagcaagga atattagaca gggtggttct ttgacaatat
    45601 taggaacagc attggttgaa actggttcta ggatggatga tgtaatattt gaggaattta
    45661 aaggtacagg aaatatggag ctacatttag atagaaaatt agcagaaaaa agaatattcc
    45721 ctgcaataga tatttacaag tctggtacaa gaagagatga tttattgctt gatgatgaag
    45781 aaaagactgc tctttggaga ttaagaagag aaatgagtaa taattcagta atggaaatta
    45841 cagataaagt cattgaactt ataaagagaa ctaaggacaa taaagagttt gtaaaatcaa
    45901 taaagaattt gtaaaatcaa taatacaaat gcaaatatgg attatataaa ctaatagaaa
    45961 aatcacttga atggcataat aagttgtgct ataataatgt agttgataat gtaaaaagaa
    46021 agttaatata attaattttt ttaaataaag agaagaggtg actataatgc aaaaggaaat
    46081 acaaccaaaa tacaatccag ttgaagtgcg ttgtgcttgt gggaacacat ttgttgctgg
    46141 ttcaactaag gacgaaataa aagttgaaat atgttcagag tgccatccat tctatacagg
    46201 aaaacaaaag aacatagaaa aaggtggaag aatcgacaaa ttcaagaaaa gattcaaaat
    46261 ggactaattt ttataaaagt ggagttggag gaagccaact cctattttat tacaaaaaaa
    46321 cattcatatt ctactttaaa ttgtcttttt gtattataat aataaatatg taaaattgag
    46381 tattctaaat acaataaaat gaggtgagtg gatggcaaaa caagctgtag gtgggcaagc
    46441 tgttattgaa ggtgtaatga tgcagtcaaa agacaaaaga gcggtagctg ttagaaaaag
    46501 tgacggagaa atagcactaa aagaagatag aataaaaagt tgggtaagag ataagaatat
    46561 agataagata ccttttgtta gaggttcttt tgttatgata gatacaatga ttcaaggcat
    46621 aaaaagctta aacttttctt cagagttttt tatggaagaa gctgaagagg ataaatttga
    46681 tttatttata aagaaaattt ttaaagataa ggcaaatgat ataattataa tattttcatt
    46741 ggtcatagca atgttacttt ctgctggatt attcattttt atacctacat tggtaggagg
    46801 agctttttct aaagtaatgc ctaatgattt tatgcttaat ttaatagaag gaatcataag
    46861 gatagctata ttatttgcat atatagtatt gatttcacga agtaaggata tagaaagagt
    46921 ctttcaatat catggagcag aacataaatc tatatattgc tatgaaaatg atttagaact
    46981 tacagtagag aatgctagaa aatttaaaag attacatcct agatgtggta caaatttcct
    47041 atttatagtt atggctgtat caataatatt gtttgcattt tttggttggc caaatccaat
    47101 tcttagaata tttatgagaa ttatatgtgt gcctatagta gcaggtttat cttatgaggt
    47161 aattagagtt cttgggaaat atgataatgg atttactaaa attattgcat atccaggaat
    47221 gatgttgcaa tattttacaa caaaagagcc tgatgatgag caattagagg ttgctttgga
    47281 agcattgaaa gcagtagtag attagagaaa aggatatttg ttatgacgat aaaagatata
    47341 attattaaat attcagataa actaaaagat ataagtgata ctccaagatt agatacagag
    47401 ttattgttac aaaaaacatt gggtgttgat agattatata ttcacttaaa tctgaataaa
    47461 gagctaactg aagaacaaaa aacaaaattt atgggttttg cagaagaaag attaaatgga
    47521 agaccaatag catatatagt tgaaaatagg gagtttatgg gattagattt ttttgttaag
    47581 gaaggcgtat tgattccaag accagataca gaaactttag tagaagaaat aattgaaata
    47641 tgtagagaaa aaaaagatgt aagtatatta gatataggaa ctggttctgg agctattaca
    47701 ataagtttag caaagtacgt tgaaaattct aaaatcatgt cttttgatat atcagagaca
    47761 gcattagaaa tagctaaaaa aaatgctatt ataaatgaag ttggtgaaaa aataaagtat
    47821 attaattctg atttatttac agcaataagt gatagtaaca taaaattcga tatcatagtt
    47881 tcaaatccac catatattaa aaaacaagat atagaaactc tgcacaaaca agttaaagat
    47941 tatgaaccat ataatgctct tgaaggtgga gaagatggat tagactttta tagaagaata
    48001 acagaacaag gtaagaaata tttaaataag tgtggaatat tagcatatga agttggtcat
    48061 aatcaagctg aagatgtaat aaatattatg aaaagtaatg gatataaaaa aatatacaca
    48121 aagaaggata ttcaaggcat tgatagagtt gttataggtt acaatatatg atataatcaa
    48181 caattgatat gttacattta atcgaggtga aagtatgtta aaaaaattag aggtattgga
    48241 agatacatat aaagacttaa gtgaaaaaat aggtgatccg gatgtaataa atgatcaaaa
    48301 agtttggcag aaatatataa aagaacatgc tgacttggaa cctattgtta tgaaatacag
    48361 agagtataaa agcgttttag atagtataaa agaatcaaaa gaaattttac aagaagagtc
    48421 agatgaagaa ttaagagaat tagctaaaat ggagttagct gagatggaag aaaaagtagc
    48481 acctctggag gaagaaataa aaatactatt attgcctaaa gaccctaatg atgataagaa
    48541 cgttatagtt gaaattagag gtggagctgg aggagatgaa gcagctctat ttgctggtga
    48601 tttatttaga atgtactcaa ggtatgctga aagaagaaga tggaaaatag aattattgag
    48661 tgcaagtgat actggagttg gtggatataa agaagtatct tttatgataa aaggtaaagg
    48721 tgcttattca agattaaagt atgaatctgg agttcataga gttcaaagaa taccatcaac
    48781 tgaatctggt ggaagaatac acacatcaac ttctactgtt gcagtattac cagaagttga
    48841 agatgtagag gttgaaataa atccaaatga tttaagaata gatgtttttc gttcttcggg
    48901 taatggagga caaagtgtca atactactga ctctgctgtt agggtaacac atatacctac
    48961 aggtgaagta gtatcttgtc aagatggaaa atcacaatta aaaaataaag agcaagcttt
    49021 aaaaatatta aaagctagac tttacgataa agcattggca gaacaacaca aagatatagc
    49081 agcagaaaga aaaagccaag tcggaacagg agacaggtca gaaagaataa gaacatataa
    49141 tttccctcaa ggtagaataa gtgaccatag aataaattta actttatata agttagatgc
    49201 attcttagat ggagatatag atgaaatgat agatgcttta attactgttg accaaacaga
    49261 aaaaatgact gcaatataag taatgtttta aagaagctgt ttcttcctag aaattatttt
    49321 ctattttgaa acagctattt tgcattttag gtcatgttat catattctat tgtatataat
    49381 tgttatatac aagtatagga gggttatatg atactaaaag taacaataat tgggcttttg
    49441 gcaggtgtta tagggacagg attaggagga gtaatatcag ccatttttaa aagagaggtc
    49501 gataaatatc taagtttttt tatgggatta tctggtggaa taatgttggc tgtagtggtg
    49561 tttgatttaa tgaaagaatc catggacaaa atggggataa tcaatacagt tatatttacg
    49621 tttgttggag ctctcataac tatgtacata aaaacaaaat tagatgtgtc tggaaatatg
    49681 gcatctggat atcttatatt cataagtata ttactacata atttaccaga aggactagcc
    49741 attggttctt cttttatgtc aactgaaagt ttaggtatta cacttgctat agttataggt
    49801 ctacataata taccagaagg gttagctatg gcattaggct tagtttgcaa taaaatgaaa
    49861 ctaagtaaag ttattttgtt tactgtaata gctggattac ctatgggtct aggaagtttt
    49921 ttaggtgttt attttggagg agtatttact tctcttatag gagtatttct tgctacagca
    49981 ggaggtacta tgatgtatgt tgtattggaa gaaatttttc cacattcaaa aagtgtgtat
    50041 tgtataattg gtttcttgtt aggtacaatg attgtaaatt atatataaaa ttaaaatgaa
    50101 agcaggcata caaaagatga aaactattat aagtaatatt gatataaata atattgatta
    50161 tgaggaaata aaaatacaag caaaacttct tagagaaggt aaaacagtta tttttccaac
    50221 tgagacagta tatgggttgg gtgcaaatgc attagatgaa aatgcagtaa agaagattta
    50281 tgaagctaaa ggaagaccta gtgacaatcc attgatagtc catatatatg aaaaagaaga
    50341 agtatatgac ttagccaaag atataagtga taaagctaaa cttgttatag aaaagctttg
    50401 gccaggacct ataacaatta tattaaataa aaaagatata attccataca aaactagtgg
    50461 agggcttgat acagttgcaa taaggatgcc ttctaatgtt attgccaggg caataataaa
    50521 agaagctggt atacctattg cagctccttc tgcaaatatc tcaggtcgcc catctccaac
    50581 aaaagcaaaa catgtatatg aagagatgaa tggaagagtg gatggaatag ttttaggagg
    50641 agacagtaat tttggattgg agtcaactgt attggattta acagaagaga ctcctatgat
    50701 actgagacca ggaagtataa ctaaagaagt tttagaggaa cttcttggag aagtaaagct
    50761 tgacccatca ttaagtaaaa aagaagataa tcaaaaagct aaagctcctg gaatgaaata
    50821 taaacattat tctccaaatg cagatgtata tataatatct ggaaaaagag aaaatgtagc
    50881 aacaaagatt aatgatatga taaaatctaa tagagacaaa ggtctaaaga caggtgtaat
    50941 gtgtattagt agaaaccgag agttttatga tggagaagta atcgatttag gaaactctct
    51001 tgaagaagtt gcaagtaatc tgtttgatgc tcttatagaa atggacaaaa agggtgttga
    51061 tgtcatatat tcagaagagt tccccaagac tggagttgga caagcaatta tgaatagact
    51121 actgaagtca gcaggataca aagtgataaa ggcttaagag ggagttgtaa aatatgaata
    51181 tacttattgt gtgtacaggt aatacttgta gaagtcctat ggctgaggct atactaagga
    51241 aagcaattaa agaatctgga agaagtattg aagaatattc tatatcatca gcaggtatat
    51301 ctacagcaaa tggaatgggt gcttctgaaa attctataga agtactaaaa gaaatcggta
    51361 tagacttatc aaatcataga agtaaagtaa ttactaaaaa attaattgat gaatcagata
    51421 taatactaac aatgactaaa tctcataaag aaattttagt acaagcagtt ccaaaatgca
    51481 aagaaaaagt gtatactttt aaagggtttg caaataaaaa tgaggaagat atatctgacc
    51541 catttggtgg aaatttggat atttatagaa gcactatgag agaaataatg tattctgtca
    51601 acgaaatagt aaaaaaaata tgaaacggag gataaacaaa tgaagatagg attaggctgt
    51661 gaccatggag gatataattt aaaaaaagaa ataataagct atttagaaag taaaggtata
    51721 gagtgtgtag attatggaac taataatgct acagattctg ttgattatcc tgtttatgga
    51781 gaaatagttg caaattcagt tataaataaa gaagtagatt atggaatatt atgttgtggt
    51841 acagggatag gaatatcttt agctgctaat aaggttcctg ggataagatg tgcagttgta
    51901 tcagatgtat tttctgcaaa aatgtcaaaa gcacataatg atgcaaatat gttatcatta
    51961 ggcgaaagag ttttaggaaa aggtctagca cttgagatag ttgaagcatg gattaatacg
    52021 gattttgaag gagatagaca tgctagaaga gtaaacatga ttaaatcaat agaagaaaaa
    52081 cataataaat aataaaaatt acagttttat caggaggaat tatcaaaatg agcaaagtgg
    52141 tagagacaaa ccatccatta atacaacata aattaacttt aatgagagat aaaaatactg
    52201 gttctaaaga ctttagagag cttttaactg aaattgcaat gttaatgggg tatgaaataa
    52261 caaaagatat accattaaaa gatgtagaaa tagaaactcc tattcaaaaa acatcatcta
    52321 aagtagttgc aggtaaaaaa ttagcaataa ttccaatttt aagagcaggt cttggaatgg
    52381 tagatggatt agtaagctta atgccagcag caaaagttgg acatgtagga ctatatagag
    52441 acccagaaac attaaaacca gttgaatatt attgcaagct tcctcaagac ataggagaaa
    52501 gagatataat agtagttgac cctatgcttg caacaggggg ttctgctgta gcagctatag
    52561 atttacttaa gtctaaagga gctaaaagta taaaattagc taatttagta gctgcaccag
    52621 aaggtatagc agaagtgcaa aaatatcatg atgatgttga tatatatgtt gcatcagttg
    52681 acgaaagatt aaatgaacat ggatatataa ttcctggtct tggtgatgct ggagatagat
    52741 tgtttggaac taaataaaaa taaataacta taaatattta ttagggactt tcttatatag
    52801 tgtatataat tttatgtaag aaggttcttt tttatatatt ttttctcaaa ataggaatta
    52861 ttgaattgat aaatttctcg aattgagaat aaaaagtttt taccaattaa aaaagattgc
    52921 ttattttaca aggttttata gaatagtagt ctattttaat gtggcatgtt agttgcttac
    52981 gattaagatt ataggtatta agtgtattag ttaaattgag aattgaatat taataatatt
    53041 attgggagtg gtaatacatg aagaattgta tagtagtaag aggggctgga gatttagcaa
    53101 cagcagttat tcataaatta cacaacagtg gttttaaagt agttgcgtta gaaatagaag
    53161 agccattagc tataagaagg caaatttctt tttgtgaggc tgtttatgaa aaaaaagtaa
    53221 ctgttgaagg tgtagaatgt atattttgca atactgaaaa agaaatagag gaagttttat
    53281 ctcaacataa agtagccttg attatagacc caaaaggaga aaaattatct aaattaaatc
    53341 caatcgtttt ggttgatgga atactcgcta agaaaaactt aggtacaact aaagatatgg
    53401 caccacttac aatagcttta ggtcctggat tttgtgcagg agaagatgta gatgttgtta
    53461 tagagacaat gagaggtcat aatctaggaa gaataataac taatggtaga gcagaaaaaa
    53521 atacaggagt accaggagca ataaaaggtt tttctaaaga gagagttatg cattcacagt
    53581 atgatggcat aatggaaaat gtatgtaaga taggtgatac agttgaaaag ggagacctac
    53641 tttgctatat taataattat gaaaaaaaac atgaagttag agcaacaata tctggagtcc
    53701 ttagaggttt attaaaggat ggagcaaagg taaacaaaaa tttaaaaata ctagacatag
    53761 accctagaaa agaagaagtt aagaatgctt ttacaatatc agataaagct agatgtatag
    53821 ctggaggggt tttagaagcc atactaaaca atgggattta tccccaaaat taatttatat
    53881 tagataaagc tattaatatt ttattaagat gatataataa atttaataga ttaaagagag
    53941 catatgacgg gtatttaaaa tttaaatggt aaaaatgctc tttttttatt ggacattcaa
    54001 ttaacatgtg tatataatat aaaaataagt atttatttag gttgaaatct aataaatata
    54061 taataccagt acataagcaa agaggagaaa agtaaattat gagaccatca tgggatgaat
    54121 acttcatgga aattgcagaa gtagtgaaaa agcgttcaac ttgtattaga agacaagtag
    54181 gagctgtaat tgtaagagac aaacaaatat taacaactgg atataatggt tctccgagaa
    54241 atcttgaaca ctgtgaaaat attggatgta agaggcagga attaaatatt ccctcaggag
    54301 aaaggcatga attatgcaga gcattacatg ctgaacaaaa tgcgataata caagctgctc
    54361 ataatggtat tagtgtagat ggggcaactt tgtatgttac tacaagacct tgtgttttgt
    54421 gtgcaaaaat gtgcataaat gcaggtatat tgaaaattgt ttatgaaggt gattatccag
    54481 atgatatgtc tacagaatta ttaaaagaag caggaataga actcataaaa ttttagtgga
    54541 taaaatttat atgacaataa atgtcaaatt atgacactga aatacaatgg tatcaaatga
    54601 ttaaaagaaa gtatttgaaa aatcatcaga ttagtagtaa gatatatttt ataattaaat
    54661 aaatttatga gatagattaa ttacatgtta tttattttat aataatgagg gatactattt
    54721 atgagtaaaa gaaatacaca tacagaaatt gcaaagttgt ttagtttaat aagtcaaata
    54781 ggattgatga ttgtaatatc aatattagga tgtactttct taggaaaatt tttagatagt
    54841 aaattgaata ctactccagt tctgacaata atatttttat tgttaggtgt tggaggtgct
    54901 tttagtggtg tttataaaac tctaatagta tatacacgaa ggaagtgata tattggatat
    54961 aaaattactt aaccaggtaa ggtcagtaac aaaaggtata gtaatatttg atgtagtttt
    55021 aattgtgcta atgttgataa catcaacatt aagtaaacca gtattattag gacttatatt
    55081 tggctctata atagctatgt taaattttag attattggcg atatcactgg aaaaatcagt
    55141 gacatttcct gctgggaagg cacaagctta tgcatcagca caatatgctg ttagattatt
    55201 tataatggca gtcgttctaa ttgcatcagc tacagtacca catttaaata taatcggaac
    55261 agcattggga ttattgagca caaagtttgt catattatca aaaaacttta taaataaatt
    55321 aaagaggaag gaggcataag tatgaatcaa gcgcattatt atctcatagg aaactttaag
    55381 attagtgaaa caatactagt aagttgggga ataatggcct ttttagcgat agcatcttat
    55441 tttttgacta gaaacttaaa aaaagttcca actagtaaag ttcaaatatt cttagaattt
    55501 gcagtaggtg gtcttgctaa aatggttaca gatgttatgg gtaaaaatac agtaaaaaga
    55561 atgccatata tgataccata cataggaagc ttgtttttat ttttcgcatg ttcaaattta
    55621 agcccattac ttggattaag gtcaccaaca accgacttag acacaactgc tgcatgggca
    55681 atgattacat tttttatgat ttattttgca ggagttaaat ttaaggggtt atcatacttt
    55741 aaagaattgg ctcaaccact tgccataatg ttaccaatca atattatagg tgagttagct
    55801 agaccaatat cattaagttt ccgtccattt gggaatattt taggtggaac aataataatg
    55861 ggtctgtttt atcaactttt aggttttata tctagtttaa taccaggatt atcaatacca
    55921 attggagaaa tattaatacc agtcccatta cacttgtact ttgacttatt tgcaggattg
    55981 ttacaagcat atatatttat aatgttaaca atggtattta tatcaagtgc aggagagtta
    56041 gattaagaaa tcaacttata ttaagataaa aataaaataa tttaaaaaat taaagtttta
    56101 aggaggaaat aattatggaa acagcaatag tagcggcagc atcagcaata ggagcaggga
    56161 tagcagttgc aacaggtata ggtgcaggta taggtcaagg tatagcagca gctaaagctg
    56221 cagaagcagt aggaaaccaa ccagaagcta aaggtgatat aacatcaact ttattactag
    56281 gagttgctat agcagagtct tcagcaatat atggtttggt tatatcaata atacttctat
    56341 tcgtaaatcc attctttaaa tacttaggaa tgtagattta gtccttaaat attttgagat
    56401 aggtttatag gtaaatcatc tcaaacttat aatcatgaga tgaactttat catctcgtga
    56461 ttataagtag ttatgattta tggaaaggag gataattatg gaaaaagcac tagtaggtat
    56521 aacgtgggaa tttgtgttcc aaattgttaa tacttttata atatttttac ttctgagaaa
    56581 gcttttattc aagccagtat taaatataat tgaatcaaga gaaaatgata ttaaaagtga
    56641 tttggcagaa ggcgaaaaag caaaaaatga aggtttagct ctgaaaaaag aatatgagtc
    56701 aaaaatcaat tttgccaagg atgaaggtca agaaatcata aaacaagcaa ctataagagc
    56761 agagcagaaa tcagatgata tagtaaatac tgcaaagaaa gatgctcttg atataaaaga
    56821 aaaagcaaac aaagatatag aacaagaaag acaaaaggtt ataaatgaaa tcaaaaatga
    56881 tatatctaac atagcactac ttgcagcatc taaagtcatt gaaaaagatt tagataaatc
    56941 taaacatgaa gaattgatag aaaactttat aaaagaggta ggggaagcta aatgataaat
    57001 gtaatagcaa atagatatgc cgaagcccta tttcaattag gagaagaaga aaattcaact
    57061 gatgtcttat tcaaagaact agaaaaagta gtagatatga tgactaaagt tagtaaagat
    57121 ttctacaaag ttttaaaatc accattagtt tcaaaatcag aaaagaaaaa cttggtagaa
    57181 attattttta gtaaagaagt aagttctaac ataaaaaact ttttaaaagt tttagtagat
    57241 aaagatagaa tatcatattt agaagatatc gaattagctt ataaagaatt attaaataag
    57301 aaaaacaatg taattgatgg agtagcaata agtgctattc caatgagtga aacagatatt
    57361 aaagaacttg aagtcaaact ttcaaataaa tacaacaaaa atgttactat agaaaatgtt
    57421 gttgacaaga ctatactagg gggagttcta gttagaattg gaaatgaaca aatagatggc
    57481 actgtgaaaa ctcgtcttga taagatgaaa gaaaagctat ctgaagtaat atcttaagaa
    57541 tggcggtgaa cccatgaact taaaacctga agaaataagt tctataatta aacagcaaat
    57601 aaaaaattat gagaataaag ttgagttgac agatacaggt agtgttttaa cagttgggga
    57661 tggtatagcg agtgtatatg gattagaaaa agctatgtct ggtgagttgc tagagttccc
    57721 aggtgaaata tatggtatgg cacttaactt ggaagaagaa gtagttggag ctgttatcct
    57781 tggagatgac agtgaaataa aagaaggcga tatagttaag agaactggaa gaatagttga
    57841 agttccagtt ggagaagctt taataggtag agttgtaaac tcattaggtc agccaataga
    57901 tggaaaagga cctatagcgt acacaaaaac tagaccagtt gaatctgagg cacctggtat
    57961 aatagataga aggtctgttt atgaaccatt acaaacagga atcaaatcaa tagattcaat
    58021 gataccaatt ggtagaggtc aaagagagtt aataataggt gacagacaaa ctggtaaaac
    58081 atctatagtt attgacacta tactaaatca aaaaggaaaa gatgttatct gtatatatgt
    58141 agcaattgga caaaaacgtt ctactatagc tcaattagtt agctctttag aaaaaggtgg
    58201 agctttggat tatacaatag tagtttcagc aacagcatct gaatcagcac cacttcaata
    58261 tatagcacca tatgctggag cagctatggg tgaagaattt atgtacaatg ggaaacatgt
    58321 acttatagtg tatgatgatt taagtaaaca agctgttgca tatagagaaa tgtcattgtt
    58381 acttagaaga ccaccaggac gtgaggcata tcctggagat gtattctatt tacattcaag
    58441 attacttgaa agagcagcta agttatctga tgaattgggt ggaggttcaa tgactgcctt
    58501 gcctataata gaaactcaag ctggtgacgt ttctgcatat ataccaacaa atgttatatc
    58561 tataacagat ggtcaaatat acttacaacc agagttattc tattcaggtg taagaccagc
    58621 agttgaccct ggtatatcag tatcaagggt tggtggttct gcgcaaatta aagcaatgaa
    58681 aaaagttgca ggaacattaa aacttgcata ttcacaatat agagaacttg cagcattctc
    58741 acagtttggt tctgacttag atgaagatac taaaaagaga cttgctcaag gtgaaagaat
    58801 cgttgagata ttaaaacaag gtgagcatca accaataaaa gttgaaaatc aagttatgat
    58861 aatatatgct gttataaata atcatttaga ggatattccg atagataata tagctagatt
    58921 tgaatcagaa ttgtatgcat ttgtagataa taattatccg gaaatatcaa gaaaaatatt
    58981 aggtggagaa gacttcactc atgatttaac agatgcaata aatgaattta aagaaaagtt
    59041 tgttgttgaa gtataatcct ttttaaaaaa ggaggtaaat taattggcag gagctggaat
    59101 caaagcaatc aaaacacgta tgggaactgt tgaaagtaca aagcaaataa ctaaagcaat
    59161 ggaattagtt gcatcttcta agcttagaaa agcaaaagaa aaggctgaag attcaagaga
    59221 atattttaat actttataca atacagtaaa agatattgcc caaacaacac gtggaattag
    59281 aaatgaattt ttaaaagaaa gagaagtaaa aaataaatgc tatgtagtag tagctggaga
    59341 cagaggactt gcaggtggat acaattctaa tgtattaaag gaatctgtct ctcatatgaa
    59401 aggtaaaaag gaaacagtga ttactgttgg aaagaaagca catgaatttt tttctaaaag
    59461 aaactacaat atagcaaaag atataaatag tgttgaagct tgtggatatg aagatgcgac
    59521 agaaatttca caaactatta ttgacctgta taaaaaaggt gaagtagatg aagtttatat
    59581 tgcttataca aaatttaaat ctgctttatc acaagaagtt gaaattttga aattgcttcc
    59641 attagtcttt gaaaaagata cagacgaaca aggaatggca gtaaaagaag aaaaacaaaa
    59701 tagatcaaaa gtacaatata taccatcagc agaagcagtt ttaagttata ttgtacctca
    59761 gtttatatca ggtacagtat atggtggaat tttggaatct tttgcatcag agcaaggtgc
    59821 tagaagaact gcaatggaat cagccacaga caatgcgaat gaaatgctat ctaatttaga
    59881 gttaagttat aacagagcaa gacagtcagc tgtaactcaa gaaataacag agatagttgg
    59941 tggagttgaa gctctaaaat agggaggtta ggaaatggca aatgtaggta aagtagtaca
    60001 gattgtagga gctgtactag atgttaagtt tgatagtgaa caaagcctac ctaacttatt
    60061 aaatgcgtta gtaataaagt taggagataa agaaatagtt gcagaagttg ctcaacatat
    60121 aggtgatgat actgtaagat gtatagctat gagtgcaaca gacggacttg taagaggtat
    60181 ggaagttgta gatacaggag ggccaataag tgttcctgtt ggagatgaaa ctttaggtag
    60241 aatattcaat gttcttggaa aaccagttga tgggaaacca gctccaaaat ctgcacctaa
    60301 attacctata catagaccag caccggcgta tgatgaacta gaaactacag cagagatact
    60361 agaaactggt attaaggtag ttgacttact agctccatat ctaaaaggtg gaaaaatagg
    60421 tctatttgga ggagcaggag taggaaagac agttctaata caagagctta taaacaatat
    60481 tgctaagcaa catggtggta tttctgtatt ttcaggagta ggagaaagaa caagagaagg
    60541 taacgacctt tatggcgaaa tgagtgagtc tggagttata aataaaacag ctctagtatt
    60601 tggtcaaatg aatgaaccac ctggagcgag aatgagagtt gctttaactg gacttacaat
    60661 ggcagaacat tttagagatg agcaaggaca agacgtttta cttttcgttg ataatatatt
    60721 ccgtttcaca caagctggtt cagaagtttc agcacttcta ggacgtatgc catcagctgt
    60781 tggttatcag ccaacattag ctactgaaat gggtgcactt caagagagaa taacatcaac
    60841 taagaaaggt tcaataacat ctgttcaagc agtatatgta ccagcagatg acttaactga
    60901 cccagcacca gctacaacat tctctcactt agatgcaaaa acagttttat caagacaaat
    60961 atcatcactg ggtatatatc cagcagttga ccctcttgaa tctacatcaa ggattcttga
    61021 ccctagtata gtaggaaaag aacattatga agtagctaga ggtgtgcaat caatacttca
    61081 aagatataaa gaacttcaag atataattgc tatacttggt atggatgaat tatctgatga
    61141 agataagtta atagttgcac gtgctagaaa gatacaaaga ttcttatctc aatcattcac
    61201 agttgctgag caatttacag gaaatccagg tcaatatgtt cctgttaaag agactgtaag
    61261 aggatttaaa gaaattcttg aagggaaaca tgatgactta ccagagtcag cattcttatt
    61321 tgtaggaaca atagaagacg ctgttagaaa agcgaaggga agtatgtagt catggccagt
    61381 gaattttcat tgaaagttgt aactccaagt aatacttttt atgatggaga tgttgaaatg
    61441 atagtagtta gaactacaaa aggagacaaa ggggtgttga aaggtcatat accatttgtt
    61501 tcaggtatac ctgtaggaac tttaaagata aaaaaagagg gaagttttaa agaggcgaaa
    61561 atagcagaag gattcattaa tgtaactcaa gataaaactg tcatattgac agaatctgct
    61621 gagtggattt aataaattaa tttagagtat aaaaagtcat tttgaccaaa aaaaactttt
    61681 gggtaaaatg gcttttttgc gttttggtgt taataaaata acgataaaat tgtaagtttt
    61741 tagtgataat aaattcacaa aatgtttaaa tttactgcca aattatgttg taattaataa
    61801 gataaagaag atttagatag cttttatgta agtgaaaaag tcttcatata agaaatacat
    61861 ataaaatttt tataaaa
//
LOCUS       DAEMGG010000009        56770 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEMGG010000009 DAEMGG010000000
VERSION     DAEMGG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.009"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(203..1042)
                     /locus_tag="KPK24_000580"
     CDS             complement(203..1042)
                     /locus_tag="KPK24_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2298288.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YISGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1066..1824)
                     /locus_tag="KPK24_000581"
     CDS             complement(1066..1824)
                     /locus_tag="KPK24_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2298289.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1871..2344)
                     /locus_tag="KPK24_000582"
     CDS             complement(1871..2344)
                     /locus_tag="KPK24_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2298290.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2373..2789)
                     /locus_tag="KPK24_000583"
     CDS             complement(2373..2789)
                     /locus_tag="KPK24_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2298291.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2836..3888)
                     /locus_tag="KPK24_000584"
     CDS             complement(2836..3888)
                     /locus_tag="KPK24_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2298292.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKVIAVDVFDEKLDLAKGLGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4284..4964
                     /locus_tag="KPK24_000585"
     CDS             4284..4964
                     /locus_tag="KPK24_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG2298293.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5097..5417)
                     /gene="sugE"
                     /locus_tag="KPK24_000586"
     CDS             complement(5097..5417)
                     /gene="sugE"
                     /locus_tag="KPK24_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG2298294.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5799..6626
                     /locus_tag="KPK24_000587"
     CDS             5799..6626
                     /locus_tag="KPK24_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG2298295.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7447..8121
                     /locus_tag="KPK24_000588"
     CDS             7447..8121
                     /locus_tag="KPK24_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2298296.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARISAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8109..9269
                     /locus_tag="KPK24_000589"
     CDS             8109..9269
                     /locus_tag="KPK24_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2298297.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKNQINNINLF
                     FDENIILRVVENIISNAFRYARNNISILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYKDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9486..10013
                     /locus_tag="KPK24_000590"
     CDS             9486..10013
                     /locus_tag="KPK24_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG2298298.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            10299..10967
                     /locus_tag="KPK24_000591"
     CDS             10299..10967
                     /locus_tag="KPK24_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298299.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKGYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            10970..13438
                     /locus_tag="KPK24_000592"
     CDS             10970..13438
                     /locus_tag="KPK24_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298300.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYTLSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITNSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13678..15102)
                     /locus_tag="KPK24_000593"
     CDS             complement(13678..15102)
                     /locus_tag="KPK24_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2298301.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15121..15813)
                     /locus_tag="KPK24_000594"
     CDS             complement(15121..15813)
                     /locus_tag="KPK24_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2298302.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15865..16644)
                     /locus_tag="KPK24_000595"
     CDS             complement(15865..16644)
                     /locus_tag="KPK24_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG2298303.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(16641..17411)
                     /locus_tag="KPK24_000596"
     CDS             complement(16641..17411)
                     /locus_tag="KPK24_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG2298304.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17408..18112)
                     /locus_tag="KPK24_000597"
     CDS             complement(17408..18112)
                     /locus_tag="KPK24_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2298305.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18367..18879)
                     /locus_tag="KPK24_000598"
     CDS             complement(18367..18879)
                     /locus_tag="KPK24_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298306.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSDCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            19205..19462
                     /locus_tag="KPK24_000599"
     CDS             19205..19462
                     /locus_tag="KPK24_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298307.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDCEQAESLINSKLR"
     gene            19605..20231
                     /locus_tag="KPK24_000600"
     CDS             19605..20231
                     /locus_tag="KPK24_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG2298308.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20478..21257)
                     /locus_tag="KPK24_000601"
     CDS             complement(20478..21257)
                     /locus_tag="KPK24_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG2298309.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21325..21786)
                     /locus_tag="KPK24_000602"
     CDS             complement(21325..21786)
                     /locus_tag="KPK24_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2298310.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21762..22439)
                     /locus_tag="KPK24_000603"
     CDS             complement(21762..22439)
                     /locus_tag="KPK24_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2298311.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22672..23058
                     /locus_tag="KPK24_000604"
     CDS             22672..23058
                     /locus_tag="KPK24_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2298312.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            23061..24860
                     /locus_tag="KPK24_000605"
     CDS             23061..24860
                     /locus_tag="KPK24_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG2298313.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25215..27089)
                     /locus_tag="KPK24_000606"
     CDS             complement(25215..27089)
                     /locus_tag="KPK24_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2298314.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(27094..28758)
                     /locus_tag="KPK24_000607"
     CDS             complement(27094..28758)
                     /locus_tag="KPK24_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG2298315.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(28772..29593)
                     /locus_tag="KPK24_000608"
     CDS             complement(28772..29593)
                     /locus_tag="KPK24_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2298316.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29692..30456)
                     /locus_tag="KPK24_000609"
     CDS             complement(29692..30456)
                     /locus_tag="KPK24_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2298317.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30453..31202)
                     /locus_tag="KPK24_000610"
     CDS             complement(30453..31202)
                     /locus_tag="KPK24_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2298318.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(31706..32311)
                     /locus_tag="KPK24_000611"
     CDS             complement(31706..32311)
                     /locus_tag="KPK24_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2298319.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            32490..33128
                     /locus_tag="KPK24_000612"
     CDS             32490..33128
                     /locus_tag="KPK24_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2298320.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            33260..33922
                     /locus_tag="KPK24_000613"
     CDS             33260..33922
                     /locus_tag="KPK24_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2298321.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            33922..35103
                     /locus_tag="KPK24_000614"
     CDS             33922..35103
                     /locus_tag="KPK24_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2298322.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(35284..37944)
                     /locus_tag="KPK24_000615"
     CDS             complement(35284..37944)
                     /locus_tag="KPK24_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298323.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(37941..38624)
                     /locus_tag="KPK24_000616"
     CDS             complement(37941..38624)
                     /locus_tag="KPK24_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298324.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(38968..39921)
                     /gene="blaCDD"
                     /locus_tag="KPK24_000617"
     CDS             complement(38968..39921)
                     /gene="blaCDD"
                     /locus_tag="KPK24_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG2298325.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(40462..42576)
                     /locus_tag="KPK24_000618"
     CDS             complement(40462..42576)
                     /locus_tag="KPK24_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBG2298326.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTCELTQLIDM"
     gene            complement(42660..43496)
                     /locus_tag="KPK24_000619"
     CDS             complement(42660..43496)
                     /locus_tag="KPK24_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298327.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            complement(44080..44442)
                     /locus_tag="KPK24_000620"
     CDS             complement(44080..44442)
                     /locus_tag="KPK24_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2298328.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(44676..45140)
                     /locus_tag="KPK24_000621"
     CDS             complement(44676..45140)
                     /locus_tag="KPK24_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2298329.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(45569..46990)
                     /gene="nhaC"
                     /locus_tag="KPK24_000622"
     CDS             complement(45569..46990)
                     /gene="nhaC"
                     /locus_tag="KPK24_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG2298330.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(47020..48084)
                     /locus_tag="KPK24_000623"
     CDS             complement(47020..48084)
                     /locus_tag="KPK24_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG2298331.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(48069..49478)
                     /locus_tag="KPK24_000624"
     CDS             complement(48069..49478)
                     /locus_tag="KPK24_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG2298332.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIKYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(49478..51682)
                     /locus_tag="KPK24_000625"
     CDS             complement(49478..51682)
                     /locus_tag="KPK24_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG2298333.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(51676..52050)
                     /locus_tag="KPK24_000626"
     CDS             complement(51676..52050)
                     /locus_tag="KPK24_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG2298334.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(52128..53558)
                     /locus_tag="KPK24_000627"
     CDS             complement(52128..53558)
                     /locus_tag="KPK24_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG2298335.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            complement(53551..53859)
                     /locus_tag="KPK24_000628"
     CDS             complement(53551..53859)
                     /locus_tag="KPK24_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG2298336.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(53980..55047)
                     /locus_tag="KPK24_000629"
     CDS             complement(53980..55047)
                     /locus_tag="KPK24_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG2298337.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKINFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(55303..56676)
                     /locus_tag="KPK24_000630"
     CDS             complement(55303..56676)
                     /locus_tag="KPK24_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2298338.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYTLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT        19254 a         9672 c         6740 g        21104 t
ORIGIN      
        1 atgaaaaagc acctcattct gattaaaaat ctttctgaga tactttttaa aatattaaat
       61 actacatata gcttacataa gtttaaaatt taatctatta taaaattacc ctaattgcca
      121 aactttttaa tataaatttt ccaacatcct acaaatataa tcaatagata tagaatccta
      181 acttacacca atatatattt atttaaaata gtcctaaaat acctgtaata gctcccacta
      241 ctatcagtat aaacattatt tttattggag aaagaccttt ttttaaagca aagagtacta
      301 ataaagtaag taccaaagga attaatcctg gaaatatctt atcaagtaaa tccgtttgaa
      361 gactaaattt aaaatcacca ctaatataac ttattggtgt agataaattg ataaacctac
      421 ctacaagccc ccccatgact aaggtaccta gtatagaagc cccttctata accttattta
      481 ctattccacc ttgaagtata ctttcaacag cttttttccc atacttatat ccatttgtcc
      541 acattccaaa agaaattgaa gtcattatta tatattgagc tattacaaat aatataggtc
      601 ctgcaattga tgctcctccc tcagttaaac caatacaaac agccaacact ataggagtta
      661 taactccttg agttaaagta tctccaattc ctgctagtgg acccatcaag cctgttttga
      721 tagcattcat accatctcca cttatttcat ctgctccatt agctttttct tcttccatag
      781 ctattgttat tccatgtata acacatccac atataggttc tgtgttaaaa aatgctagat
      841 gagtctccac ttcttgtttt aactcttcct tatctggata taatttttta agtactggca
      901 acattgaatg tgaaaaagct gttgattgca atctttcata attgtaatta gattgtgcaa
      961 agaaaaacca tcttaaccat gattttatta catctttctt gcttaacgtt ttcttagata
     1021 cttcattttt tatatctgac ataatatttt ccccctcaca tgtatttaag atgtagcact
     1081 atcttttccg tacttaattg aagtaattgt aagagcaagt attactccaa taacagatat
     1141 tactattata tctactttaa aataaacagc cattagtatt cctaatatga aaaaaggcat
     1201 agtaaatttg tttccaatca attttaaatt catagcaatc ccaagtgctg gcaacatagc
     1261 cccaattaca ttaaatacat gtaacacatt atcattcatt acttccaaca taccatctat
     1321 cgcatttgga ccaaaataaa caactaataa tgctggtata aaactcattg caaataacag
     1381 tatctgtggt ggtactatat ttataaatgc tactctatca acattccctt tttgaacctc
     1441 tctatcagcc caatgtgcaa aaaaagaatt tactgtcatc tttccaatcc aaagtacagt
     1501 tgcaacagtt cccaatggta cagctattgc cattgcagta cttacatcta gcttagatgc
     1561 tatagctaaa gcagtaccta catatcctgc aaaagctggg tcccccatct gagctccccc
     1621 tgctgatata aaacctagat atatcatatt tatacctgct cctataattg ctccttgaac
     1681 agggtctcct aatataattc ccgttacaaa tccagccaat agaggtctat tccatgtaaa
     1741 aaatccaaca taagtcaacc acggactcca agaaagataa taaaaaattg ctattaatac
     1801 tgcttgaatt agtgaaatgt gcatattctt cctccctatt ttcttttaaa gatttacaag
     1861 atagtcttct ttacaataat gttcctacat caattttctt tgcatctggt actatttgta
     1921 taaagcattt aacatctttt gatattagct ctctaaatat ttctttttct tcatctgaca
     1981 cagatatatt tttataaaaa ctttttctac caagccctgc tcccatacct ccaacattca
     2041 attcctttaa atctaccccg ctatttacta aacctaagac agttttaggg tatttgacca
     2101 atactattaa attttcatct ccattatgtg attttaagac ttcagcacct gattctacat
     2161 catgtacttc aaccttaaca gttgatggag ctgccatttg taaaacttga actataaatg
     2221 ggtcctttac tactaaatca tccacgatta ctatcctttt tgccgatgta atcttacacc
     2281 aagcagtaat aacttgccca tgtattaatc tatcatcaat tcttgttaat gctatattac
     2341 tcataactat tcctccagat ttttaattta ttttacaact ttaaaacagt tttaacatct
     2401 ttgattcctg atatcccaac ttcctcacat aaattcttca agtcttttaa cttcatagta
     2461 tctctgttta aaaataactc taaaagcata ggtaaattta cacctgtcaa acattctaac
     2521 tcacatacct tagcaaattt atttattata atagcagatg tattaaatgg agttccgcca
     2581 taaaaatcaa gcaatactaa aaccccttca tccttatatt tctcaattcc atcttctaac
     2641 tttctactaa actcatctat gctgtcacca tggtttaatc ctaaagtttc tacattttcc
     2701 tgtttgccta ctattaattc agcactttct aacattgctt tagccatatt tccatgtgtg
     2761 gctataatta ttcctgccat atgaatcata cctttcttaa ccttttttat atggattatt
     2821 atttagtact ctaatctaat tatctatatt aatcattatc ttaccaaaga aaatatccct
     2881 tttgtatact ttttcaaaat aagctggtac ttcttctaaa tttattctat gtgttactaa
     2941 tttttcaaca ttcatatcac ccttttgcat atgatacaat ccagcactcc attcttttcc
     3001 tgggaatgga aaggagtttc caaaccaagt tccctttaca gttaattcac ttcttactat
     3061 cttttcaaat tgctctctag tcaaagctac atccccatat ggtacacctg catacaatac
     3121 tgtacctccc tttttagcta gcaacagtac ttgaccacat gtcagtggag taccagccga
     3181 ttcaataaca atatctgcac catctccatc agttaacctt tttatttctt ctacaatatt
     3241 cttttctttt gcatttatac aaatatctgc ccctaaccct tttgccaagt ctaacttctc
     3301 atcaaataca tccacagcta ttacttttgt agaaccaaat atttttgccc attgtattga
     3361 aaataatcct attggacctg taccaagtac tacaacagta tctccaactt tagcttctga
     3421 cctaaaaaga ccatgtcctg ctatacatac tggttctagc gcagcggcag tctcataact
     3481 tatctcatct ggtattttta ttaaatttct ctctttcacc tttgtatact ctgcaaaaca
     3541 acctccaagt tctttatttc ctattattgc tacattatta catcttgagt acaagccttt
     3601 tttacattca tcacattcaa aacatggcat agctggacaa acagcaactc tatctcctat
     3661 tttaaaactc cttacttctt taccaacctg tgccacttct ccagaaaatt catgccctaa
     3721 aatttctcca accatatgtg gacctgtctt tgaatatttt gatatatcag aaccacatat
     3781 tccagctact tttactttaa ttattacatc atctttttct aatatttttg gtacatccac
     3841 atcctccact cttgtgtctc ttataccata gaatctaact gacttcacta ttaaccaccc
     3901 ctacttttta tttgagaata tatccctatt gtttatatgt taaatatcta aagcaacttt
     3961 catgccaata tatgtaagca ttgtattcat gcattttata aaattatcat taaatgtgtg
     4021 tcaaagtgtg tcaaaagttt cttatattaa aacttatgtg tgtcaatata aacaacacta
     4081 acattttata ttagttctct tattataagt tttataaaat gtactttttt tgttattttt
     4141 ataaaattgt tcggaaattt cccttctatt tgtgatataa tattaaatat aaggtatcaa
     4201 tataaatttt catgcgaata tcagaacttt tttgacacta atgccttatt caatataata
     4261 atttaacttt ttggaggttg tttatgaaaa aagtgtatca aggaaagact aaagatgttt
     4321 ttgaattaga taatggaaat tacttactta aatttaaaga cgatgtaact ggtaaagatg
     4381 gagtatttga cccaggcgaa aatagtgtag gtctaagtat agatggaatt ggtagagcta
     4441 atttagagac atctgttaag ttctttgaaa ttctaaacaa tgctggaatt aaaactcatt
     4501 atgtaagtgc taatttagaa gaagcaacta tggaagttct accagcaaaa gtttttggta
     4561 atggattaga agttatttgt cgttatagag ctgtaggtag tttcttacgt cgttatggtt
     4621 catgtgttga agaaggtgct aaactagatt gttacgtaga agcaacttta aaagatgatg
     4681 accgttgtga ccctcttata acatctgaag gtttagaagc tttaaacatt atgactcaag
     4741 ctcaatttga ttcaatgaaa agcatgactc aaaaaatttc taacatagtt cgtgatacta
     4801 tagctgaaaa aggtcttgag ctttatgata ttaaatttga atttggattc tacaatgatg
     4861 aagtaatctt aatagatgaa atagcatctg gaaatatgcg tgtttataaa gatggtgtaa
     4921 tagttgaccc tatggattta actgctcttt tattagataa atagtatata catataattt
     4981 acaaatataa taaacttata aataagataa gctatgaagt aaagactttt taaatgttga
     5041 ttatttttaa acattttctc gcctttaatt tcatagctta tttaaattaa tatactctaa
     5101 ttagtagata atagctttaa ccctacaatt cccaccacaa taaaacctat acatgcaatt
     5161 cttgatatac taataggctc atttaaaata gccatgccta caatagctgt accgacggta
     5221 ccaattccag tccatattgc atatgaagtt ccaagtggaa gatgttttag tgctaatgac
     5281 aaaaaataaa aacttacaat cattccaaca acagtaagta tacttggagt caatttagaa
     5341 aatccatgtg aattttttag ttgaaaagcc caaaacactt caaacaaacc tgcaacaaac
     5401 aaaaataacc atttcataaa ttcatccctc ttttcaaata tgtctcttat ttttccaata
     5461 aaataacctg agaaatatta taaaatatct cccaggtttt tgtccttccg tgttcacaac
     5521 aaaaactatg ttgtgggttt tatcttggac cagaccaatc attaataatt gcggaaccct
     5581 ataaaactct attaattatt ctattttatc tataataata ctatcaacat atataaaaaa
     5641 agtcaatata tgggttccat ctataataaa aataactttt atataccttt taatattctt
     5701 attcacttct ctccataccc atttgggtca atctaagaat atctaatttt tatttaatat
     5761 gttataataa cataaaaata ttgaaaggag taatttctat gaatttctat aaaattgcag
     5821 tatgtcagat gattactaca gaaaataaaa ttgaaaatat caatcatgct gtagatatgg
     5881 taacagaagc tgccatcaat ggagctaaaa ttgtagtttt gccagaaatg tttaactgcc
     5941 catatgaaaa taaatatttt cctaaatttg ctgaagaata tccaggtgaa acaacaacga
     6001 tattaagtaa attagctgag aaacatggaa tttatctagt ttctggtagt attcctgagt
     6061 tagaagatgg aaaaatctat aatacttgct atgtatttga taaaaatgga gctctaattg
     6121 gaaaacatcg taaaatgcac ttatttgaca ttgaagttac aggaaaagtt agttttaaag
     6181 aatcagatac tcttacagct ggaaatgatg tcactgtaat agataccgaa tatggaaaag
     6241 ttggtatagc tatttgctat gatattcgtt tccctgagtt atcacgttta atggctctta
     6301 aaggtgctga aatcgttatc ttacctgctg catttaatat gactactggt cccgcccatt
     6361 gggagttatc tataagaatg cgtgctttag ataatcagat attctatgtt ggtgctgcac
     6421 ctgctagaaa tatgaatgct tcttatatag cttttggaaa ttcaagaatt tcagaccctt
     6481 ggggaagaat aattgctcaa gctgatgaaa aagaatgtat tatttatgct gacattgata
     6541 gagatttaat tcctgacata agacaacagt taccacttct taagcacaga agaactgatt
     6601 tatatgaact taatactttg aaataaaaca gaacataacc ccataaatcg taaattaagt
     6661 gttaaaaaaa tttatggggt tctcattaat ttgatttaaa attaatttca taaaaataat
     6721 gctaaaatta atttaattaa ataatactaa taattactaa tgattatgct aattatcagc
     6781 atttactaat taatcactat tagttatttc ttttattaaa tatctttact tttttactta
     6841 ttatgtcttc tagctttata tttggattat ggtatttctt tctattttta gtcacatctt
     6901 tctattgaat tacttgctta aaacaccaat gcacacatgc cagataatgt tcacaattat
     6961 gtagaaatct tgctctacct tcttcaatgt tgatattatc tctcctagtt gatattatct
     7021 ttcctaaaat ttcttttaat taaaaaacaa cattagttga tatatcaagt atattttaag
     7081 caactgaaaa aaacaataat aattgatata tcaactaatt aatgtagatt aattcatttt
     7141 tataatttat atacttttta attaatctac cttatttaat taaaatcctt cctttctatt
     7201 tatccatttt caacttaaat tataagattt catcagctat atttttgcat accaataatt
     7261 attaaatatc aaatatatat tctattaaaa tttctaataa tttcatcctt gatttattta
     7321 atatataaaa atatactaac tttaatgatt aagttttgaa atataactaa aaatttgaga
     7381 atttattgag atttttatac taaaatatac atactataca aacttaattt agaagggagc
     7441 attcttatga acaataaaat actcgtcgta gatgatgagg aaggtataat aacactctta
     7501 aaagattact ttgaaatgaa tggttatgag gtttacactg ctcaaagtgg aaaagacgca
     7561 ttaaaaaaaa tatccaaaaa tccagatatc attctacttg atataaacat gcctgaaata
     7621 gatggaattg aagtttgtaa tagaattaga gattatgtat catgccctat tctatttctt
     7681 acagctagaa ttgaaaatac tgacaagata aatggttttc gtgctggtgc tgatgattat
     7741 attgtaaaac cttttgattt agacgaactt ggagctagaa tatctgccca tttaagacgt
     7801 gaacaaagaa agtcaaatca aactaaaatt aaattttata atgatttaac aatagattac
     7861 gaaaaaaggt taatttgtat atataataat cagatttcac tttcaaaaaa ggaatttgat
     7921 atagtagagc ttctctctat gaatcctggg caggtatttg ataaagaaaa aatatatgat
     7981 agagtctggg gatacaatag caatggaaat agtgatgtta taatggaaca cattagaaaa
     8041 atacgaatca aactatcaaa acatactctt gaaaattata tagaaacagt ctggggagtt
     8101 ggttataaat ggataaaata aataatatgt cattaaaaaa agctttcttt gttcttactt
     8161 tatcttcact aattatagca tcagtactta ctactttcgc atacatttta cttaataatc
     8221 tatataattc tatacaagat aagtatttaa aaacacctat aaatacaggt attataacca
     8281 tagttcaaaa caatgtgaac caatttagtg attatgataa aacattactt aatataatca
     8341 acatacttca gcttgtactt ccacttatat tttttatagg acttttatta ttagctgata
     8401 taatttttta caaggtaaaa ttaaaaacac ccatagaaat actcaataaa ggtgcaatgg
     8461 aaatatccaa caacaattta gacttttgct tagagtataa taataatgat gaattaggta
     8521 atctatgtaa tgcttttgaa aaaatgaggt ctgaactaaa taaaaacaac ataaagatgt
     8581 ggactatgat taatgataga aaacaactta atgctgcttt ttctcatgat ttaagaaatc
     8641 ccctaacagt tctaaaaggt tactcaaact atttaacaaa atacatacca acaggtaagc
     8701 ttagtgatga gaagatttta tcaaccacac aacttatgtc tgagcatatt aacagaattg
     8761 aatactatgt agaaaatatg agtaatgctc aacgattaga agatttagtt gtttcaaaat
     8821 ctatgtcgaa tataaacaag tttatagaaa acttagatga aaatatatct atcattgcta
     8881 aacaagaagg aaaatctttt acacttaaaa atcaaataaa taatataaat ctattttttg
     8941 atgaaaatat cattcttaga gtcgtcgaaa atataatatc gaatgctttt agatatgcta
     9001 gaaataatat atccatactt atatatcttg aacaagaact ccttaccttt gtaattgaag
     9061 atgatggtat aggatttagt aaggaatctc taaaattagc tttaagacct ttctataaag
     9121 ataagacttt aaatgacagc aatgcacact ttggtatggg tctttatata tcaaaaatat
     9181 tatgtgaaaa acatggtggc tctatttcta ttgaaaataa ttctactaac agtgctaaaa
     9241 ttactgctaa attctcaaca agaaattaaa atgattttgt aagctttcac tgttcttata
     9301 taagctttgt tagtttataa tttataaaat gtatttcaat ttacaaccaa tatatatact
     9361 attttataga agttgtaaga gttgcttcaa ttataatatt ttggcaaaag gcatttgaaa
     9421 caataataga aatatatatt taaatatgta gaatagtatg aaattagaat tttggaggtg
     9481 attatattaa aagttttgct aataatatta cattctctcg tattgtgttt gcattgctaa
     9541 tgcttagttc tacattctgt tcactgcctt tctggatatg ttatttttgg tgtggaatta
     9601 gtgatatact tgatggtctt attgcccgaa agttgagaca acaaagtgga ataggtgcaa
     9661 agttagatag tattgctgat ttcatttttg cggttacaat tttggttact gtcggaaaaa
     9721 atattgctct tcccatgtat ctatggtggt gtattgttgc aatagcattg ttacggttga
     9781 ttagttacag tattggtttt tttaagtatc gcacattttc atcacttcat actttgatga
     9841 ataaaatcac tggaatttct atctttgctt ttccattact ctatatttgc tttggtcttg
     9901 atgttgcagg aattatgata tgtatcgtgg cttttctttc ttcttttgaa gaaatagcaa
     9961 ttatgataaa atcaaaagaa ttaaatcgtg atataaaaag tatatttatg tgaaaactta
    10021 gaatttgtga caaaaataat ttatataata atgaggaagt aaaacataaa aaattatcca
    10081 caagtttatg tgtaaaatag ttttaaagca atagagttgt attgtaaagc ctttagtgca
    10141 taaattacat tttaaattaa aaatgatact gaaactgctt atcagcactg cgaattatcc
    10201 gcaaatagtg aagacttttt agatttaagc taaaagttgg gaaaaaattg agttttaaaa
    10261 cttacactct tcttatatag aaaaggagag tgttttttat gattataaaa gcaaaacaat
    10321 tatcaaaaat atatggttct aataacaata aagttatcgc acttaataat gtaaatttag
    10381 aaataaactc aggtgaattt gtttctgtaa ttggaccttc tggaagtgga aaaagcacac
    10441 ttttacatat tttaagtgga ttagataatc ctacctctgg acaagtgtta cttgatgata
    10501 aagatatata taaacacact gaaaaagaac tatctgcact tagaagaaaa tcctttggat
    10561 tcgtctttca acaatttaat cttttacctg ttcttacagc atctgaaaat atttcaatgc
    10621 ctgtacttct agataaaaaa caacctgata aaggctattt aaatgaaatt tcatcattac
    10681 ttggaattgc agatagactc aatcatcttc cccatgaact ttcaggcggt cagcaacaaa
    10741 gagttgcaat tgctagagca cttattgcaa aaccagatat aatatttgct gatgagccta
    10801 caggaaactt ggacagtaaa agtggcagtg aagttatgaa tcttcttata aaaacttcaa
    10861 aacagtttgg aaagacttta gtggtaatta ctcatgatga tagaattgca aaacttgctg
    10921 atagaaaaat ttcaattatt gatggtgttc ttatggaggt gaaatagaaa tgaagaatta
    10981 cctttcactt tcaataaaag aattaaaaag ccagaaactt atgactacat tcatcattat
    11041 agcaatagta ctatcaacta ttatgacaac tgtggttgga cagtcaattg gtatcctaca
    11101 aaatttaaga attgaacaag cccgttcttt taatggtgat agacatgtat cctttcacca
    11161 actcactaaa aatcaagttg atgacataaa gaaagatgat agagtttatc aagctggtac
    11221 ttcaataact attggcagta gtaaaataaa ggattctggt atctctgtac ttgtaaaaga
    11281 gtatgataaa acaggacttt ctaactaccc aaaattaatg aaattaaaat ctggacattt
    11341 gcctaaagat aaaaacgaaa tagccttaga tgaaaacaca ctaaagctta tgggtataaa
    11401 accaagacta ggagtgacta tacctatgaa tttagatatt tcacttttaa atgataccat
    11461 accaccatat aattacacag ctaactttaa attgagtggc attttagagg atgattatac
    11521 aggatatgta agtggtatag taaatggtat tgtaggtaaa ggtacatcag aaaatctatt
    11581 acctaagaga tatactttat cctctttgga ttttaaaata aaacagcaag aaaagtttca
    11641 agaaatagta aatcaattag ctaaaaaaat aaatctttct cacaattcaa ttcaatataa
    11701 ttggatttac ttaaatgctc ttaagataca gtttgaaaaa gatgaaaata gttctaatag
    11761 tgatggtata tctatgatta tattggtatc tttatttgtt gccttattgg ttttactggc
    11821 ttctggactt gtaatctata atattttaaa aatctctgtt acaaagaaaa ttaaagagta
    11881 tggatgttta agagcaatag gagctgaacc aaaccagata tataagatag ttattttaca
    11941 aatcttaatt ttatgtactg ttgccatacc tattggagct gtaattggaa ttatatcttc
    12001 gaaaggcata actggaatgg tcactaatat cttaaatcca gatatacttc tagcaaatga
    12061 taataaagaa attactgagc ttatacataa aaatacaaca gcatatatgt tcccattagt
    12121 attaagcact aatgtatctt tgattttttc acttatttca gcattaccat cagcaatata
    12181 tgcttcacat gtctctccca aaattgctat ggctggtagt actaccaaaa tcaaaagaaa
    12241 aattaaaaga gaaaaaacaa taaaaaactt tgaaagacat ctagcatggc taaacttaaa
    12301 aagaaataaa ggaagaacta taataactat actatcatta tttatgagta taacagtatt
    12361 cgttgcactg agtgagttct ctaatgtatt agatgtatct aggtcagtaa gtaatttaaa
    12421 agaaggagat ttttccttaa caaatgaaat atctggtttt gataaatcaa cactagataa
    12481 aatagctaaa atgaaaaacg tcaatcgcac ttcctttata aagtactctg aatacaaaca
    12541 gggtgaaata gatacagata taaattttga aaatagtggt gagatgttaa aaatcattgg
    12601 aattgatgag caaactttaa aagacttaat gccttctatt acaaattcta tactagaaga
    12661 ctttaaaaat ggttctatct gttttataaa aaatccgatt gctatttcta caccaggtgt
    12721 aaaaactaga tatacaaatt taaaacctaa agatagtatc acaatcaaca aaaaacaact
    12781 agatatatat agtacagtag ataaaatgtt ctttttacaa ggcaatggct ggataaatgg
    12841 tgttgatgtt attgtatatg attctgttta taacactttg acaaataaaa acaagttcaa
    12901 ccagataaat atttatgcta aagataaatc aaaattagaa caaataagat tgtctattga
    12961 acagatttgt gaaaataatc ctggctctca ttggatatca tatattgaat cagataaaca
    13021 acttaaagag agttttaaac aaatagaatt tctagcatgg gcagttatat tatttgtggg
    13081 attgattggt actttaaaca tcattaacac aactcataca aatataaata ctaggactaa
    13141 tgagattgga gttaaaagag ctattggtat gagtaatagg agcttatata aaatgttttt
    13201 atgggaaggt gtttattatg gaatttttgc tgctatattt ggttcaattg ctgggtatgc
    13261 cagtgctatt atcataaaca tggcaactat agaaaagctt gatttcacta atataccaat
    13321 tacttcaatt ttacaagcta ctattatttc tgtacttgca tgtattatag ctactcttat
    13381 tccgttaaga aaagttaaaa agatgaatat aattgactgt atagatatca atttataact
    13441 attttacaga taataatata aattaaatta tcaatgactg tctctatttt ctttcaaatc
    13501 taactaataa atctttttaa ctataatata agctttaaaa aaggcctgtc tcaaaataga
    13561 gataagcctt ttttcattgt cgaaataatg aaaatatatt taacttcaca aaaaaagagt
    13621 gcctcacgaa ttaaaaattt cgctttgaga cactctcctt taatttaaaa tatatagtta
    13681 aatgctatgc caaaatttca cacatgctcc acctgatttt gaattttcta ttttaacaga
    13741 acctccaagc tgttccacta attgtttgac aatgtaaagc ccaagaccag aatgtccacc
    13801 ttttgtctgc ctagcttcat ctcctcgata aaatttatca agagcctttt ttaaatcttt
    13861 agagctaaac cctctaccag aatcacaaat ctcataagaa atacaatttt tttcatcctt
    13921 tacagtaata ctaatattac cacctgatgg agtgtattgc agactgttgg atatgatatt
    13981 atcaaaaatt cgtgtcaacc tatcaacgtc gatttgaatt ggggattgta tattcccctg
    14041 cattttcaat ataagttcaa cttccttttg atgagcctgt aattcatagt cttgcacctt
    14101 ttgcctcaaa aattcaggta aatttatagg aacaagattt aattgtatat ttgacttttc
    14161 taagtcagac gtatattgca tcttttggac aagagcagca cttttttctg tattttctct
    14221 tatcactgta agatacctat gaagtttttc attgtcatct gtattattac caataagtgc
    14281 atctgtatat cccaaaataa tagaaagagg agattttaaa tcatgtgcta gagcttccac
    14341 catttcaaca cgttcctgct ccatcttcca ctgtgcagat aaagagcctt ttaattcgtc
    14401 tttcatttca gaaaaggcac tacaaagttt accgagttca ttatctgaat aatagtcgat
    14461 ttcaaaatca aggtcctttt cttttatctt cttggacgca tcaatcaata gatgtaatgg
    14521 ttgattgata ttctttacaa agcgcttaga aagccatcta gtaaaaccca cgatatatag
    14581 aaatggagaa aacaaagcca ttataataac tgcaaataca aatctccctc taatatttgc
    14641 aaaggtgatt ttcacttggt agaatagaat aacagcaccc tcaatctttc cattatcccc
    14701 tttgattgga acagtatgga tataaccacc tttacgaaca gttttattga taaaattatt
    14761 aaataattct tcttttgttt taaatagttt tttggggtta gtaccatata aaatattgcc
    14821 attattatcc acaacttgat atagcatatc atccccacga atcgttcttt ttaaaccttc
    14881 ttcattcgat tgtgataaca aagctatgtt tttttcattg atgtattttt caattctagg
    14941 aacttgtcgc tcataatagt ttggtggata gatatcctta tttaaggaat gaatatacaa
    15001 atagagagca aatacataag taatcactgt ggcaactata ctggctatta taataaaaat
    15061 aaaagtatgt ctaaattggg taataaggga ttgatttttc atttaataac ctctctctct
    15121 ttacatttta ttccacttat acccaatacc ccaaactgtg gaaatatatt ctttttctgg
    15181 tgtcacagca gaaaacttag ctcgtatttt ttttactcgt tcaacaacag ttgaggaatc
    15241 accttcagaa tcacaccccc atactttttc atagatttgt tctcttgaga aaacttgtcc
    15301 tgcatggagt gctagtaatt caacaatatc atactctatt tttgttaaat caatattctt
    15361 accatcaatt ttaattgcac gttccttcat atataagcat aacttcccaa aatacaaagt
    15421 agatctcttg ttctcttcat tgatatactg tgaacgcttt tccctacgca gattagcttc
    15481 aatccttgcc ataagttcac gcagtccaaa tggctttgtt acataatcat cccctccaaa
    15541 ggtcaaccct ttgattttgt cggtttctga ttgctttgca cttaaaaata taattgggca
    15601 taaaacatca tctctaattg cctgacatac ctcaaatcca ttcattttag gcatcataat
    15661 atccagtata ataaggtcag gctcctgttt tgataactct atacctgcct gaccatcata
    15721 agctatcaac acttcatgtc cttttgcctt caattcatcc tcaagtagca ttactaggtc
    15781 catttcatca tcaataatca atattttact caatataaca ccacctttct cttatgggaa
    15841 ggtcagtatt aaaacaatat tcgtctactc ataacttttc cttccctccc acattttaaa
    15901 ccaaataacg cctcctatca agaatataat caaacctatt gctactacta taagctctat
    15961 actagctgtt tgcatcactt tagagtaaaa tgcctcagtt gaaatagttc cagcttcttt
    16021 aataaaataa attcctggaa gcatacctag ttttactggc catgtccatg gaacaaatgc
    16081 ccatactttt tcaccaacgc tagttaatcc aagtattgct gccatcaaaa ttccaccaat
    16141 actaatacca atagatgcac ccatacccca tgcaaaactc acccaaagat gaatagctaa
    16201 tagtggaagt gtcccaatca ccacaagtcc tgctgctgac aggaataacc atattgttgc
    16261 tccagatggc actgcaatac tcattcctat gcaaagtatc aacgtagcaa taaatgtgca
    16321 tatagttagg caaaacagaa gaaaaagaaa ttttcccaga tataatctga cacgagaaat
    16381 acctacacat aaaaaaccat taaaatttcc agcaagctct tcctcctgca ctataaatcc
    16441 tgctaaaata cctactccaa taggaatgat gaatacagtc catactgtaa aaaatccttc
    16501 aaaagcaaat tcttgtgtac ttccagattt agttgtgaga taaaaaacca cacataatga
    16561 aaaaataata ggcatacaaa gagtaagcca ctggacagct gtcctctttg ctcgaagcca
    16621 ttctgatgat aatagtttta tcatttgtca tccctcctat tgaaccaagc tgcagtaagt
    16681 gctgttaata ctataaacac aaccaaagaa acaacaatcc caataggaat cactgaagta
    16741 ttcaaaagtg ggtctccagc ttctaatata gtaccgttag gatgtatgtt gatgattggg
    16801 cataccattc taactgccca actccaaggt actgcaatcc aaaatggttt tgtcgcagca
    16861 agaacaccaa atatcatacc taaagctcca actcccatac ttaggaacat acctttccat
    16921 gtagctagcc atagctgaag tggaagtata gcaagagaaa caacccagca tacaatactt
    16981 gcccctataa tctgtccaaa aggaactgga ccagctttca atattaaacc agttataatt
    17041 gttacgacta ttaaaactag cgttgaaata aaactgtaga ctgccatagc tataacttta
    17101 ttaatccaaa gtgtcatagg agacacattg tgtgtacgta aagcacgata atttccagct
    17161 tttttttcct ggaaagctac caaagtagcg aacaatgcaa atccaatagg cagaaataaa
    17221 aatgaccacc agttaaaaat taatgctaaa agattttgcc acgcccaaga agttgtttga
    17281 gataatggat tttgcatgag tgttgattgc attataaatg catatgctgc aaaagacact
    17341 gggaaaaaaa caataagttt acccataaat gttcttttat actttaaaag ttctgattgt
    17401 aaagcattca tctgttttgt cctcctttaa gaataacatc tgtaaaaaat tcttctagat
    17461 tttcattagg gtcaggcatt ccttgaaata atagtcgtcc tccatttata attcctatat
    17521 tatctacaac ttgagcaacc tctgatagaa tatggctaga aagaattaca gttatacctt
    17581 ttttgggaaa agaagcaatc aattcacgca attcttgaat gccaaagggg tcaagaccat
    17641 tggttggttc atctagtatt aacaactctg ggtcactcaa aagtgcaata gctattccta
    17701 acctttgttt catacccata gaaaattgtg aagctctctt tttacctgta tttttcaaat
    17761 ttactatttc caacacttca taaattttat ctttaggtat tcccaatatt tttgtatgta
    17821 ctaacaaatt ttcctctgct gtaaggtttc catacaatgc aggggattct attaaagaac
    17881 ctatttttgc aagagattct cgtttccaag gctttctatc aaaaataata cgaccatttg
    17941 ttgggcgaag aagaccaaca agcattttaa gtgttgtgga ctttcctgct ccatttggtc
    18001 ctaaaagtcc atatatacta tttcgttcaa tttgtagaga aacattgtta actgccattt
    18061 gttctccata tctttttgat aagttttttg tttcaagaat taaatctttc atattaattt
    18121 ctcccttctt gtaatacctt aaatctgtta ctaagagaat actaacatga aaaaataaca
    18181 attctataac acctactaat tttaataaaa tttaaaataa aaaatagagt taccaatttt
    18241 attttggcaa ctctatttaa gtttatgttt tacagttata ttagatttaa acttagtttc
    18301 attatcacac taacttcaaa tttactctat aatctttaaa tcaaacacta atttattaat
    18361 caaacactaa tctattaatt tatatttctt tagacatcta atatagtttg ctactaaata
    18421 ataattattt atatcatatt tatctaatcc tttaaaagaa gttatttcat cattaagctc
    18481 atcactacct tctacaaaaa cacaacctgt ttcaaattcc atacctacaa taatcttaca
    18541 gtcactataa atttcctctt gagtatcgta caattttttt gcatttcctt catttggaat
    18601 tgtaaataca ataggtttta attctttatc actggaaatc ttttgtgaaa aaggcttgtt
    18661 taacttaata ttattgataa ctacatgttt aaattcctct gaagtttctt ctactattac
    18721 atattttcgc tttaaaaact ctattatttc taaaacagac ttagaattta cttttaaaat
    18781 acttttatat ttttcatata aagatttcca ctgttcaatt ttttcctgtg gtatttcttg
    18841 gatatgtgta cttatttttt cttgagatat ttttttcaat taaactctcc tttcaatact
    18901 taaatatcct tatgaatctc acatgactaa agttacaagg tttctactta atagaatttt
    18961 gcatacttaa atatgtctta ttaattctct acaggatatc tccgtagttc ctacgattct
    19021 tacataagtt acttatttta aattgttatt agttatattc taaaaattat ttattttatc
    19081 actaatataa tactcttaaa aactttacaa atcaatttaa atttgctacc ttttatttat
    19141 aacaacttat agtacatttc tcctatgctt ttcaatatat tacttttaaa aggaggtttt
    19201 cattatgatt ttaaatagag ctgagctttt ccgaggatat tctttaaata gatttaacga
    19261 gatatgcaat atattatcag taaataaaat taaatataag tataaagtga aaaataatgc
    19321 ttactctaaa aaaccttttt ttaatgaaat aacacttggc aatttaggcc aaaaggaaga
    19381 tttctcttat gagtacttaa tttatgttca taaagacgat tgtgaacagg cagagtctct
    19441 aataaattct aagttgcgat agattatcta taaaacatga cattctgttg tcatgtttta
    19501 tatgttatta tactttcaaa tatgctacta tattttcaaa attgtgtgct ttaaaactag
    19561 ctttttttag taatttaaag cttaaaacta aggagactcg ttatatgaaa ataaatataa
    19621 aatcaaagtt aagtgaattt attaaacaga acaatctttt tgatgacagc agaataatat
    19681 cctatcttcc taatataaca atagaaacag acaaaataaa aactataatg attaatgagg
    19741 ttgtcccaac taacaccaat gacgattttt atagtgtaga taaagatgct gattatttaa
    19801 aaactactat tcctttattt gacagtgcag gaataaaagt atctaacata aatgatatcc
    19861 tagatatggg tatctatata actaatgcag taaaacttcc taaatcagaa tatacaataa
    19921 ctcgtgatac aattaaatta catatgccta ttttagaaga agaaattaaa ttatttaaaa
    19981 acctagaggt agtaatgtta atgggagatg ttgcaaaaaa atcttttaat atgattacta
    20041 aaaagcatat aaaaaagaat gtcatccctt ccatttcaac atataaactt cgtaataatg
    20101 aactttatta tgataattta agagtatttc catcatatat tatgactggt ggaaacatac
    20161 tgattgaaaa atcaaaattt gaaatggcat cagaagatat aaaaaaaatg ttcgagatta
    20221 taaacggttg atattacaaa ataataaaaa ctaatataaa ataaaaagtg cctcaaaaac
    20281 ttctatttat gaggcacttt tattttatat tagtgtaact atatactatt aacataattt
    20341 attatttata ttgttctttt taatctctat tccatttagt ctaggtaaca ttactcttta
    20401 caagattact ttatatttct tttatatctc taacgttttt atttaataaa acaattatta
    20461 atacaagtca aatatatcta tttatctaaa aatatatctt ttacagtaat atctggatgg
    20521 tgatacttct tcctattcgt taaaaaagat ttttttacat taatagcctc ttttggacac
    20581 cagtgaaaac aagctaggca tctttcacac tcattacccc actttggctt tttatcaact
    20641 atacttatat tctctacagg acaaaccctt ttacatgtcc cacatccatt acatttattt
    20701 tctgcaaaaa atttagtagt tgcaatttgc atattcttac ctactaaacg aagtacaaca
    20761 ctatctatat gtgactttaa attgtttttt ccttctatat ttccaatctc caagttattg
    20821 atacattttg caatctcagc taccctatta gtctgatttt tcaatctttc ttcttcaaca
    20881 tctggaggtg ttatcaaagc atttccaggc atattgatta aaaaaccagc agataatttt
    20941 ttcccccttt ttttaagtaa cttatctata gtaaataaac tatgtccagc taccccatta
    21001 caagtggcaa tggcatatat atgagtatta atattaaaat ttaatttctc tacaaaagac
    21061 tttactatat caggaacatc agcaaaataa acaggaaaaa caattaccaa tatatcttca
    21121 tttatgtcta tattattttc ttttctatgt atagctaagg gtataacttc tttacttcca
    21181 tttaacgttt cttttaaaat atttgaaact actagcgaat ttcctgttcc agaaaaataa
    21241 taaatcttac tattcattct tatccctctt tctatatttc tacatcatca atagttaaaa
    21301 agtctcttaa aataggattt atatttactt tccaattcta tccaaaatat cagtaaacaa
    21361 aggcaattct tcatcagaga tagatttaaa tgtctctgtt gcatatatac ttgcaatttc
    21421 atttaatggt tcttctaatt ctctacctaa atctgttagg aatatttgaa acttacgtct
    21481 atctttaaca tctactttac ggacaattaa tcccttattt ttcataacat ctaaaattct
    21541 tgttgtatta gatttatcct tacatgattt ttcacataaa tctatctgag aaatcccatc
    21601 ttgctcccat agaaacctta ataacgcata atggtcagtt gttatatcat atccattttg
    21661 tttaaacatc ttttttaaat catcttttac ttttaacgaa gtacttacta acttagttgt
    21721 agcttcatat agtttctcaa aatctttcat tttacctcca tttaaataat cccttgctcc
    21781 tcccatctat caattattcc cttatattgc tcaatatctt taaaagccat tggctttaaa
    21841 acagcattta tagcaataaa attatagatt tgagaaagca caggtggatt tttcttttcc
    21901 tctatgcaat aaataaattt tttagatacc ttttgtatct ttgattctac tttttcagtt
    21961 ataggatttt gattaaatcc agtttttata gataaactac cacaaacata acctcccgaa
    22021 tctaagacca tggattttat aaaagaaatt gcttttgaac cacgtgcatt ttcatatgta
    22081 gtaactgcta tacatggctt acgatacaat aatctttcaa gcatcatatg tgctctatct
    22141 gtaaacacct tgaaaagtcc agttacacta cttccataag ttgggctacc aaatataaca
    22201 ccatcactct tagtaattat atcatgaata tactctactt tatcattttg attgatacac
    22261 ttgcccattt tataacaatt taaacaacca atacaatatg atggatttac ctcacttaaa
    22321 tcaaaataat taatttttac atctggtatt aatctttcta tatctttata caagtaagtt
    22381 aaaacttttg atgtagctcc attttttcta gggcttccat ttataactgt aattatcatt
    22441 tctatatctc ctttttagtt gttatatcaa caataagtat atcaactata attttagctg
    22501 tcaatataaa ccatttattt aaagctatac ttacacagct taaataaaag ttttatttaa
    22561 ctacatgtgc ttatttaatc aactataaat gaattatttt taataattga caaactaaat
    22621 taatgataat atactttatg tattacaaat gtaagtagaa ggaggtatag tatgaaaaag
    22681 ttaccacata tttcagaagc agaatatcaa gttatgaaaa ttatttggaa atatgctcct
    22741 atcagcacta ctgaagtaat tgaaaagttg gtagaaacaa gtacatggag tcctaaaaca
    22801 attcaaacaa tgttattgag attagttaaa aaaggagctt taacatatga aaaaaatagc
    22861 agagtattcg tttatactcc acttgttaaa gaagaagaat acgtagctac agaaagtagt
    22921 tcttttttaa atcgttttta taatggtact cttaattcta tggtactaaa ctttttagaa
    22981 aatgataaac tatctgaaga tgatattgag gaactgagag aaatcttaaa taaaaggacg
    23041 actaaggggg ataaataaat atgtatgccc cattctttat acgattttta ctcagtacat
    23101 taaccttatc aatattaatt gctgtaattt tacttgccaa aaaagtattt aaaaaacata
    23161 tttctgcaaa gtaccaatac aatattggat ttatattgct tataatgtta ataattccat
    23221 ttgtccctct taaatatatt aatcttggaa atgtatttaa ctatttatat acttttaatc
    23281 aacttggaag cagtactaat aggacttcta tgataaaaaa tagtccaaat tctataatta
    23341 ataactcaaa tgcattaaac gatttttcag tatccctaaa ccaagttaat tttgatagtt
    23401 taaatatttt attactaata tcatggataa ttggaatttt aatcatgata gctattacta
    23461 ttcattgcaa cattaaaata aaaaatatga aaaaatcaat acaaatttta agagatgagg
    23521 agatagtatt tatatttgaa aagtgtaaaa aagatattgg tataaataag gattttatac
    23581 tatgcaaatc acctattata aatacaccta ttacatttgg atactttaaa ccacacatta
    23641 tattaccaga taagtctata tctaaattat ctttaaaaga tataaaatat atcttacttc
    23701 atgaattaca acactttaaa aataaagata tactgataaa ttatataatg tgcttccttc
    23761 aaatattata ttggtttaat cctcttgtat ggtatgcatt taaagaaatg cgtatagacc
    23821 gtgaaattgc atgtgatatt tcagttttaa aaaagcttga taaagatagt catatagaat
    23881 atggacaaac tctaataaac tttgtggata atatttcaag accatcaaat cttacactta
    23941 catcagatat tggtgggtca aaaaaacaaa taaaaaagcg actattgaag ataatggatt
    24001 ttcataatga atcaaatacg ttaaaattca aaagcatttt aatttttaca ttgatagctt
    24061 taattgtatt tggaaatatt cccaaactat ctattatggc agatatcaat gaaaaatata
    24121 gacctacaaa tgaacaaact gtgtacgagg acttaagtca gtattttaaa ggttttgatg
    24181 gaagttttgt tatgtatgat ttaaaatcca agcaatatca tatttacaat aaagaaaaaa
    24241 gtaaaacaag aatttctcca aattctactt ataagattta tagtgcctta ttgggattgg
    24301 aaaatgggat aataacaaga aataattcac atatcccttg ggatggaaag catcattcta
    24361 atagttcttg gaatgaagac caagacttat tttctgctat ggaaaactcc gtaaactggt
    24421 actttcagac tctagataaa aaaacagggc taaaaaacat acaaacttat cttaggcaaa
    24481 ttggatatgg caattatgat gtttcaggtg gtatctcaaa cttttggcta gaatcttcat
    24541 taaaaatatc acctattgaa caggtagaat tacttgaaag tttttataca aataagttta
    24601 aatttgaaga taaaaatata gaaacagtaa aagattcatt gttaatatca aaaaaggatg
    24661 gggtatcact ttttggtaaa acaggcactg ccaatataaa tggaaaagat gtaaatggtt
    24721 ggtttatagg ttatgtggaa aaaaatgaaa atacttattt ctttgctaca aatattcaaa
    24781 gcaaagatta ttctaatggt agttcagcat ctaagataac attatccata ttaaatgaca
    24841 aaaaaatcta taatccttaa tatatttact gtacattcta aaaaattcac ataattttca
    24901 tattaattat caattaaatt catatacaag gcttattata taaacataga agatagtaaa
    24961 actagcttaa aaacataata taatacccta tacaaatagt ggcaactctc cctcacatcc
    25021 tcaattgttg ccactattta attttttgta tgaagttaaa tatattttca tcatttcaac
    25081 aatttgggtg caatacaatc tctattttga aacaacctct ttaattgttt tagagtttat
    25141 gaattatttc tcatacttta aaaagattga attactcttg ataatcttct tttgtagtca
    25201 ttacatatca cctactactc aacacatatt ctttgaccta tttttacatc cttgtttata
    25261 tccaaaatat ttaccttctt accatctgga aacaatacca ttgttaaagt agactttcct
    25321 aattcatgta ttctctttaa atcaacatta acaagaacat ctccctgttt aaccatatct
    25381 ccttgtttaa cagcaatatc aaagccatct ccatttaatg caactgtatc aattccaaca
    25441 tgaattaata cttctccatt agttccttta atcccaaatg catgtccact tgaaaccact
    25501 gtttctaatt tacctgaaat aggagaaaca atttttccat ctataatatc aatagcaaaa
    25561 ccatcaccta acattttttt agaaaatgtt tcatctggta cattttctaa tgcaacaatt
    25621 ttccccttag ctggagacac aaagttttca ttattttcat taaacactaa ttcttcgtct
    25681 tcatcttcat ttatagcttc tttaggcact ccaaataaac atgttacaat aaagcagaat
    25741 gcaaccacaa atattgatgc taataagtat gtgattaaat tatatccatc atagatatac
    25801 attaaataag aaggtaaaac tgctaatcca tatgaattag attgaatatg taaaattgat
    25861 aatattgctg ctcctaaacc tgaagatgca cacataataa tcaaaggctt taaattatat
    25921 cgaagctgaa tcccaaataa aacaggctca cttataccaa aaagttgaga tagcattgct
    25981 ccaactgcag tttgtttaac ttgattattt ttagattttt tcataaatgc tagacatgtt
    26041 ccaatattcg caaatccata cattgcacaa agagtaataa gggcattaaa tcctgtattt
    26101 gctaatagtg atgtttcaat cattacataa gtatgatgta agccagtaat aacagctaat
    26161 ggatatgtaa atccaataat aaatcctcca attcctaaag gcaaatcaat caataagctg
    26221 atgatatcaa ccatttttaa ttctattgta tgcattatag ggcctacacc taacaaaata
    26281 actaacattg ttattagcat aacaaaaaat ggtgtgaaaa taaggtctaa tacattaggc
    26341 atatgtttac ggaaccattt ttcaagatta gctccaataa atgctgtaac aatagctgtt
    26401 aaaacacttc cttgacaacc tacaatagga ataaatccaa atgccataat tgcttcaatt
    26461 tcatttgatg ggtcagcaac agaatatgca ttaggaagta ttggggaaac taacataagt
    26521 ccaataacaa gtccaataac aggtgttcct ccaaatactt taaatgctga ccatacaata
    26581 attgctggta aaaaagcaaa tgctgtttct gtaagaaccg aaacaagtgt ctgaatatac
    26641 aatggaatat ttgcagaaga cattccaaat aatccaagta cattatcatt aaacaaacaa
    26701 ccctttaatc ccaagaataa tcctgttgct gcaataactg gaataatagg aacaaatata
    26761 tctgctagag ttctcataac tttcttaaat ccagtttcat tatttttaac taattcatct
    26821 tgttcttttt ttgataaagt ttttaatccc attttttcaa cttctgcata aactttatta
    26881 actattcctg ttcctaaaat aatttgatac tgtccagaag taaagaaaac ccctttaacc
    26941 tcatcaactt tttcaacttt cttatcatta ataatttctc tatctttaac catcactctt
    27001 aatcttgtgg cacaatgtgt aatagaaata atattatctt caccaataat tttaataagc
    27061 tcatttgcta ttttattata tttattcatt tttctacctc actttcaata atactgcatg
    27121 aaatggtttc aaacgcactg ttttatttaa tagatttata tcatctaaat tttggaatac
    27181 aatatcatat ttttcttcta ctcttatttc tacaggttta tcagaaaaat tataataaca
    27241 atcaataact accaaatcta attgccttct atacgcaata atatcatcac tttctatatc
    27301 aataggttct attgaaccat aaatcaaaca atcagaatac tcactatctt gtctcaactt
    27361 tatcatcttt ttataaaatt ctaaaatact ctctgaattg ttaatctgac tctgtgcatt
    27421 aatatcttta taattttcaa taacctttaa ccatggtgtt gtatctgaaa aacctgcatt
    27481 ttctttatct gcccatggaa atggcgttct tgcattatct cggcttctta aattcacaaa
    27541 ttctaacgct tcttcttctg agtaaccttc atctattgaa cgatagtatt ggtcaatgct
    27601 agataaatca ttaaaatcat ttatggtatc tcttttaaaa tttgtcattc ctaattcctg
    27661 tccctgataa ataaatgggg ttcctcttaa aaagaaatac attgctccca acatttttac
    27721 tgcatcatca ttcctttcgt attgcaaaag atactttgtt gtagctcttg gttggtcatg
    27781 attttcaata aaattagctg cccatccatg ttcctgaaca tacatttgac tatctaaaat
    27841 gcattttctt aaatctggaa ttgtccatga aattctttta aaccactcac taccagaagc
    27901 aacatctaag tcagcatgtt taaagtcaaa gaccattgaa aagaaaccat cttctccaat
    27961 aaaatcacct aactggtcat agctaatacc tgctgcttca gcaacagtca tacaatcata
    28021 ttttttaaac gtattttctt ttaattcata aagcattttt tctattccag gctgatttct
    28081 aactgttttt gtacatttaa caagaccatc aacaccatca ctttctaaac tttgaaaact
    28141 taaattcttt ttgatgaatg taatagcatc aactctaaat cctgccactc ccatttgaga
    28201 ccaggtatta atcatttggt aaagttcact tcttaatatt ggattttccc aattaaggtc
    28261 aggttgtttt tttccaaaag aatgaaaata atattcgtct ctattctcta ccttttccca
    28321 aacacttcca ccaaaaacag acctccagtt atttggcaaa tcgtttcctt ttttaaaaat
    28381 ataataatca tgataaggac tttctggatt ttttaatgct tcttgaaacc aaggatgttc
    28441 atcacttgta tgattaagaa ctaaatctaa tataattttg attcctctat ctttactttt
    28501 tttaattaac tcttttaaat cttccatact tccaaattca cttgcaatat ccaaataatc
    28561 actaatatca tacccattat catccatagg tgatttaaaa attggacata accacaatga
    28621 tgtaatacct aaatctttta aataatctaa acgagaaata attccctgta aatctccaat
    28681 tccatcatta ttactatctt gaaagcttct tggataaatt tgataaacga tttctttttg
    28741 ccaccatttc ttttccataa attactcctt tttatatgat atttcttctt tttaattctt
    28801 tataaattcc attatcgaat atgtcctcac atatagatgt cgctatatct ttaagttgtt
    28861 gatgactatt tttcattgca attgtaacgt ctgatgcttg aaacatgaca atgtcatttt
    28921 gaccatcacc aaaacatatg gtttcttttt gagttactcc taatctttct tgtaatctct
    28981 taattgcttt gcccttatga aaatcttttt gaataatttc ataatactga ctagaaatat
    29041 ctctctgtgc taattccatt ttatcttgta gtatatcttt cacttcgtca aatacctttt
    29101 catttgacca taaacaaatt ttatgtatat cttgtgactt atactcttca atgttatttt
    29161 catatgttat tttttcttgg atatgttgtt tatttataca agaattagtt cctttcaact
    29221 gattcattgt ttcaaatatt tctttggctt tttgattcat gaaaactttt tcctgactct
    29281 caatcgaaaa agcaacttct ctatttttta ataaacaaac aacttccttt atcaatcttt
    29341 gattaaaaga ttgattataa agtaattcac catgatattg aatgtaatta cctcctcctg
    29401 caatatatcc atcaactcct aatgataaaa catcatcttg tattgtaccc atacttcttc
    29461 cagtacaaat aacaacactg caatgattct tttgacataa acgtatagca tgctttgcag
    29521 attctgggat tccatatacc tcatctctaa gcgtaccatc tatatcaagt attaataatt
    29581 tgtacaattt catcttcctt tcttactatc aaaatcataa tagcattcaa aaaaaaagat
    29641 atcaatattt tgatatcttt taaaaaatat gttcattttt agcacaaatt ttcattttcg
    29701 gggaatatgt tcactttcta aatacatctt gtatttaata taaagcagtt caattaaaat
    29761 aaaataaggt gttgttacat cgtagtccag ttgtttatct acagaaatag atatagattg
    29821 aatatacagg ttttcatcac ataatcttgc taaaggatta cttatcattt ttgtaataga
    29881 aaccatataa attcctttca ttctcatttc tttagcaaaa tcaataattg tagatgcatc
    29941 tccattcaat gaaataaaga aaatcatatc atctgttttt ataaaatgat taagtgcctg
    30001 aatcatatca taaccataaa catcatatat aattttccct gttggtaaga aaatcctttt
    30061 catctcttta gcaactcttt cctgagaata accttctcca aagacgatta tacgatttgc
    30121 ctttttcatt ctttcaaaca taatcgaaca atccttcttc tctatatcat ttatcacctt
    30181 attatagcaa tccattaatt ggtgtttact ttgattttta tattctactt ctgtttcatt
    30241 taagcgtata ataactctta attcactata tccaggtaat tgtagttttt gagcaaaacg
    30301 agaaagtgca cttgtagata tatggtattt gtctgcaaat tcatctatag aaagtttaat
    30361 acaatcttct ttatgattaa gaatacactc acagatatac ttatcatttg ctgtaaatga
    30421 attataatat ttattgaaaa gtccttcaat tttcattctt ttctccttct atactctatg
    30481 taattcatat gaagcaaatc taatagagca taaaatgctc ctacgacttc ataagatata
    30541 gcttgtaatg tatcaagttt ttgagtttca acatacagat tatactcaca aagtgttgca
    30601 attgtattat tatcaagcct agttaaagaa atcgtatgta tatgattgtt gataaatttt
    30661 gcaatttcaa ttcctgcttt tgtttctcca gacaaagaaa tgataataaa taaatcattt
    30721 ttttgaaatg attctctcat aaattcaatt tcacctatat caaataagtc aataacacat
    30781 ttacctactg aaagaaatat tctctttatt tcttgagcta aacttttctg ttcattacct
    30841 gtcccataaa tataaattgt ttctgcatca tatattattt tacaaatatc ttgaaatgat
    30901 attttttgta attcattcat taatagtcga taattctcgc aaacatcatg aaaattatta
    30961 tcattattaa caaattctag ttgattattt ttaagtaaat actttaaatc agagaatgaa
    31021 tctaatccaa tttttctaca aagccttaat aaagtagctc tagaaacatt acattcattt
    31081 gcaatttttt cacacgaaaa attacggtat tctctttgac aaatcaatat ttgtttaata
    31141 ataattttat cattttcatt taaattatca taattttggt taactaacga ttctaatctc
    31201 attttttcca ctccctaaat attattttaa tatacattag ttgctttttc tagggaagag
    31261 ttttaaataa taaggataaa ataatcattt gattttattt ttattaaaat aaaaataacc
    31321 tgctaaaagc aggcaccaaa aagtacttat cctttcgaac atgtacttta gatattgctt
    31381 tcttcggaag cttcatctct tcatactcta aaatatagtt ttttctaact caaaagtatt
    31441 ttccatagta gaaattactt taattcatct gccatttact ttttatctta tttaataatt
    31501 actattactt gaatatttac ttctttaagt aaatttcata gttaacaaag ctaaaactat
    31561 tattatagat gttaaaatac aaaattgtaa gtattgttat aaatatgctt acagctacaa
    31621 atatacatga tatattaaaa agaagctgta aattattatc atacatacaa gcaactatgc
    31681 ctctattaat ccatgttaat ttttattatt taatacaagg agttccatga gacatacata
    31741 tccattcata ttttaaatta tccaattctc ttatagattt caccatcttt ttataatccc
    31801 aattccatgg attaggataa ggaactatat ttccattctt atttttgact aaatcacctg
    31861 caaataatac agcttcaaaa atcatgcaga catgccctgg agtatgacca ggagtatgga
    31921 taatttttat attcccaatc tgcatattct ctccataagg ttcaacatcc ttaatgagcc
    31981 ttctggaaat tgcctttcca ataaacttct taaacccagg tctatccttc tctcctataa
    32041 taaaaggtat atcctctaca tgagcccata cttttgctcc tgttttttct ttcaataatt
    32101 taatattaga tatatggtca acatcatgat gagttaataa aatatgctta atatctgtaa
    32161 gtttaatgcc aagtgttttt aattctttaa ttatattttt ccccataaaa ggaagacctg
    32221 tgtctataag aataatttct ttatcaaaca caataaaaca atttgagcca cgagtactat
    32281 ctaacattaa tacattttct tttatcttca taaataaatt ccccttattt ggtattttac
    32341 ctatataatt aattaataaa gttataaata gttataatac ttgatattaa ttttattttt
    32401 tattaaagta tactataggt atacaagtat tatatttaca taagtatacc atcagtatac
    32461 ttttttatca atagaaagga ataatttata tgtcagaatt atcaaaaaga gataaagaaa
    32521 aaatacaaag agaaaatgag attattgata aagcagaaaa attattttgt cttaatggat
    32581 ttgataatac aactatgaat gaattagcaa aagaagttga atatactaaa cgcaccatat
    32641 acaagtactt ttcatgtaaa gaagatttat tctttgctgt tgtcttaaga ggatataaaa
    32701 gactttggga taatgttaaa atagaaagtg caaaaggcaa aacagggttt gaaaaaataa
    32761 aactttcata ctttgctttt cataaatttt actctgtaga accttctcta ttatgcctta
    32821 tgggaatgat tggtattgta aactctgaaa aatcggatac tgatatgctg tttaaagaaa
    32881 agtttttttc ttttaataaa tttatgtttg atgaaataca aggaatgttt gaaattggaa
    32941 aaaatgatag aagtattagg catgatattg aaatacctac tcttatgtat tcatcaatat
    33001 ttacacttac aggatttttt aatttactat ctgtaacagg aaaatcatac ctgaacaact
    33061 ttaatataga tgaggaaaaa tttattgaaa caactcttgc tcttttaata gattcaatta
    33121 aagcataaac ctaagtttat aaattaaagt ttatatactt aaatgataat tttttctatc
    33181 tctaatataa aaaatattaa acataaaact aataacttag ttaatggaat aactaaatta
    33241 ctgaaaggat taaaagtaaa tggctaaaat acttctttta gaagatgatg atacaattac
    33301 atttggaata aaaacttccc ttgaaaagaa aaatcacact gtatgttgtt ataatacgat
    33361 tgctgaagct aaaaaaaatt ttgataaaac tattgattta attctgcttg atttaaatct
    33421 acctgatggt attggatacg agttttgcca atatgttaaa agtatagaag atactccagt
    33481 gatatttttg acagtccgtg atgatgaaaa agacattata cgtggattag atatgggtgc
    33541 agatgactat gtggttaaac cgtttttgct ctcagtactt cagtccagaa ttaatgcagt
    33601 tcttagaaga actcattcaa atactaacaa tactattaca tgtgaaaata taagcttgaa
    33661 taaggttgaa gtcaaagcat atttagataa tgttgaagta cctttgacag caggagaata
    33721 taaacttctt ctaagtctta tggaaaataa aaatagagct atgactcgta caaagttact
    33781 agaaatgctc tgggatgtag atggtaattt cgtaaatgat aacactctta ctgttacaat
    33841 aaaacgtctt agagaaaaat taaaaaatcc accttgtata aaaacgttaa gaggcatagg
    33901 atatagaatg gaggacagct agtgaaaact ccaagaaata tcaacagtac agttgttttt
    33961 ataatattct gcttaattgt aactttttta ttgagtgtaa ttctaacaac tataacttct
    34021 tttaatgaat acaatgtact gtctaaggct cttggtgcat catatgagta caatcctaag
    34081 tctacagaaa gtatactaaa atcactgaaa aattctagtg ataaggattt taaaacagga
    34141 aatgaaattt tatcaaaaca tggatattct aaaagtacat tttggagtaa aaactttttt
    34201 tattttctag tgatatcgtt tggtataatt tcaacaatgg ctacagttat atatataatc
    34261 aaacatagaa caaataagca aaaactgagt agaataaaca atcttactca atacttagaa
    34321 gaagtgaatt taggaaaaga gtctgtactt ttacgatgtg aagatgaatt ttctcacctt
    34381 gaagatgaga tatataaaac agttggggaa cttcgttgtt caaaagaatt tgctttaaaa
    34441 gaaagacaaa ctctttcaga taatcttgca gatattgctc atcaacttaa gactccaatt
    34501 acttctatgt cacttatggc acaattactt tcagaaaatt gtccagatga agaaatagat
    34561 tatattaaca gacttacaaa tcaaatctca agacttgaga gactagtttc atctttactt
    34621 acattatcaa aattggatgc aagtacttta atatttgaaa gcaaacttat agatgtacat
    34681 tctcttctta cttatgcaat tgagcctata gaaggttctt taagagaaaa acatcagaca
    34741 tttactatcg aggcttctcc ttcggtagaa tttgaaatcg atattaattg gactttagaa
    34801 gctcttttaa atattcttaa aaattgtagt gagcatatag aagatggtgg atatatagtt
    34861 gcttattata gtcaaaatcc tctatatgtt gagattatca tagaagataa tggtaagggg
    34921 tttgctcagg aagaacttcc atatattttt aatagatttt atcgtggtaa aaatgctagt
    34981 aaggacagta ttggtatagg gcttgctcta tcaaagtcta ttatagaacg tcaaaatggt
    35041 actctacatg cagaaaattg ccataatggt ggtgcaagat tcataattaa attttacaca
    35101 taaaatgctg gattataggt aaaataccta taatccagct atatctatat tctatgttat
    35161 ctattgtttt agtaatatac ttctatttac atttaaggtt ttattaaaca aacaacttaa
    35221 ctatccctat acttgtaagt agtcttaaat cgaaaccttt aaataagttc aaatagattt
    35281 tgtttatatt gtttcatttt ttatagattc tactatattt tcattcttca actgcttgta
    35341 tcctgtcata tatgatgcta ttacaataaa aagtatcaat atagtaaaac ctattattgt
    35401 agatataggt gcaaatggta aatactctat aaaatatatt tcatttatac gtagaaaagc
    35461 atacactatt cctatttgca caggaatact aagtagtaat ggtgtaattc ctaagaataa
    35521 accttccaaa ataagcatct tttttatgcc ttctggagat aatccaacag aacgcaacat
    35581 agcaaactct tgtcgtctac ttctaatatt tcctgacact gtagcaagta catttgataa
    35641 tccaatcata gccaataaac ctgtaataaa gcctactatt aaattcatgg tctttattcc
    35701 actagccttt tgtttttcac tttctaaaac atcctcaata ctataatctc ctgaaccata
    35761 gtctttatta taaatggact ctaatttttc tcttactcta ggaatgtcct cacgattatt
    35821 tactttaaat actcctatca gcctatttga acgtaaccta cttacttcat cataattttt
    35881 agatatttcc tgtaccatag acattggaac tatttgcatt aatgtatact ttgaactagt
    35941 atctactatt ttaggaagtg tgcttgtaat agctcccact ttcatgttgt aagtataatc
    36001 ccctttatct tcgtcatata ctttttcatt taatttaatg ttatctccta ttgaaagatt
    36061 taaataatct atatatactt tatttctacg tgtactacga ttaatatctt cctccttatt
    36121 atatacaatt gaggttggat tttttgtatt ataaaataac tttgggtcta cagaaatttg
    36181 cctacaatat tctgtaaaac tcttatcatc aagcccaatt atatttgaaa ttatacgaaa
    36241 cttgccatca cgttctatag gagagtattt tttagatgaa actatacttt taaatcctcc
    36301 atttttcgca agttcttttg atgcatcttt ttcagtcagc caagtggaag cagacatttt
    36361 ttgataatat agtgaacttt ttacaccttc tgtattatct aatttttcaa tgaaagcaga
    36421 ctctataggt tcactatttt ccaaataaac tgctatatct ctttggtcct tataattatc
    36481 acttccataa acagcttttg ctccttcttg atttgcaatt atatgcaaaa aacatgtaag
    36541 taataaaaaa gataatgtta aagatattgt tgcagtacga taagattttt tacgagcacg
    36601 tagtgcgttt gatgcaagtt ctccttctat tccaaatatc tttcctaatg aatatttatg
    36661 cgactttttg attttaacac tatctccttg tcgtattgct tctataggag atatctttga
    36721 tacttttcgt gctggtataa gtgcagaaat ccagactgtt actattgtaa gtaaaactgc
    36781 tggcagaaaa gcaggaatac cataagtaaa taccacttct ggaacatcag tatgataatt
    36841 aactgaatta acataaacta taagcctatt acacaataac catcctaaaa ataaacctat
    36901 tggtaatggt ataattgtca gtaaaaagcc ttcaaacact acagaccttt taatttgttt
    36961 tggtgatgca ccaacactag ataatatccc taactgagta agtctagcat ttgcagacag
    37021 tgaaaacgcg ttgtaaatca ccattacaaa cacggctact gtaaaaattg ctattgtaag
    37081 atacataata ggagttgaaa attttgatat actactcata gatgctttct gttctggtga
    37141 aaagactaac atctttaaaa gataattact attgtattta agattgtatt ttccatattc
    37201 attagtcttc catcccaatg atttagcaag ttttggaagt tccttatacg tgtctcttat
    37261 atttttaaat cgcaagtaca cagttatact atcattaggt tttatacttg ttttatcaag
    37321 atatccaaga gctgtatatg caggtattac tgatgaagta gaaacatcta cctttcctac
    37381 tattgtcaat gtagcctctg aaattttttt aaaactttcc cctttcgatt ttggggctat
    37441 gggttcaaga ggctttccat ttaagattct atatcctatt gggagagtaa ttttatcacc
    37501 aatttttaca cttggattat tctcaaaata ttgttttgat aatgcaattt cattagcatt
    37561 atttggtcga cgaccctcta ttatagtatc cttttctggc atagaattcc aatactctga
    37621 tgtagcattt ctataaatta tataatctct tttctcatca ttaattttgc ctacatacca
    37681 ttgtcctttt accattacat catcaactga agaaaaattt tcaattaatg gtaagtcact
    37741 tccataagta tagtcaaaca attcaccatg ccagttacct tctttctcaa ttgaaagtct
    37801 tataaaatct gtccacatgg tatacaacag tccacacaaa gaagacatca tggttgtagt
    37861 aagtaatata gctaacatta tagctatact actacgttta ttgcgtcgta caaagtctaa
    37921 ggtgtattct tgaagaatat tcatttcttc accacctttt cagagacaat cactccatct
    37981 tccatagtta caatacgatt tgcctctagt gctattttct catcatgagt aataagaagt
    38041 attgtctgat taaatcgttt atttgataag tttaacaagt ctacaatttc ttctgaattt
    38101 tttctatcaa gattaccagt tggctcatct gctaataaaa tagctggtct atagattaaa
    38161 cttctagcta tagataccct ttgttgttgt ccacctgaaa gttgacttgg aagatggttt
    38221 aatctatcag ataatcccaa tatagataca atctcagaga atctatcctc atccaccttt
    38281 cttttatcaa gtaacatggg taataatata ttttttctaa catcaagggt aggtataaga
    38341 ttataaaatt gataaataag acctactttt ctacgtctaa aaactgcaag agcttcttcc
    38401 tttaaacttg atatttctgt atcttcaaca taaatctttc cagatgtagg tctatctact
    38461 cccccaagca gatgtaacaa tgtagatttg ccagaaccag atgctcctac aatggataca
    38521 aaatcacctt tttgaactga taaatttaca ttattaaggg ctgtcactct ggtttgattt
    38581 gaaccatata tttttgtgag attttcacat tttaatattt ccatatacaa tcatctcctt
    38641 tatggttaaa ttttagcaac ctaaaatgac attatagtga catgaataca aatataaatt
    38701 actattaaaa atattgttta gtttttgcac attatttaaa attataatat aaaacaaaaa
    38761 ctatgcaata tattttattt atacttttaa tatattacat agtctttaaa aggtattaac
    38821 tttaaatcta taggtattca atttaataat caatagcaag tattttatgt actaaaggag
    38881 ttttgctcta tatagactca atttagtaaa attatttaat aaatattaca gactataggt
    38941 aaataatatt tacaaataaa attaagttta ttcccttaca ctataatatt tcttaattat
    39001 attaattgca atttccttta cctttggccc tgtaatttct ttattggaat catctgattt
    39061 aatagcaaaa taataggtgt caccttcacg ctctagcata ccaacaaacc atgcatccac
    39121 acgtttattt ttttcatcaa atcctgttcc agtttttcca tatacattta tgtttttatc
    39181 atttatatct attttcataa tatctcttaa aatattaata tgctcttttt taaagtttgt
    39241 atctccctca aatatttttg ccattgtttg cacttgttct tttggagata tttgtaatga
    39301 agattctaac caaaatccat ttaaatgtcc ctttccattt tttaaatcgc cttcccattc
    39361 gctaatatcg caattcccat actttaaatc atctaaaatg ttttggacag atttagcatc
    39421 aactttatct atcaattttt tgtaatacca aacacaagac tccttaaatg cctcttctaa
    39481 actaagattt ttattccaat ttttatttgg atactctgta ccatcatatc ccataactga
    39541 ttcttttgag tttattaccc ctttttctaa accaattaaa gttgaaacta ttttaaaagt
    39601 agaacatggt gaccttcttg tttcaattaa ttctttgtta tatatattat attctttatt
    39661 tttagtatta taaaaaatag ctcctccact aataccttca aaacaatcac tatagtcaac
    39721 catattaatt ttttcttttt tgtttttatc attgttttca attttggatt tatttaattc
    39781 agacttattt aattcagatt ttatataatt cttatcagtt ttttgattta tatcgttatg
    39841 ttttttccct acataataca ttaccataac tacaccaacc aataagatag ctatccaaat
    39901 aaaattcttt tttcttttca taatttatat catctcccat atttaaattt ataacctact
    39961 attaaaatag tagacattat ttgtaatata ctccaatcta ggttctactt ataagaaaca
    40021 agactaaatg aatagctgta tctcttatat tttcttcacc tagtacaatt atgttgatta
    40081 aacctattgc gaagataatt aggtaactat taataacatc ctgtcctcct tttaatttgt
    40141 gatataataa ttgattccac atttaaaact tactattgta atatttaaat attacaatag
    40201 taagttttaa atgtcaagtt agtttaggaa agttatttta attgtataat tgttattaca
    40261 tttataaaat atggcatgtt taaaattatt ttgctattgg gaggattaag ataagcagta
    40321 ttataaaagc tataaattat aaaatttaat atataattta tagcttttac ttttacaaat
    40381 attgttttta ttgatttatt aatgaatttt attgacaggt tatatttata taagtaaaca
    40441 cttacataat aagtatataa tctacatatc tataagttga gtcaattcac aagtatatgt
    40501 caacttaagt ttatgcatag ctcgtgtaca ggcaatatat aacaaactgc ggtcataatc
    40561 actataatat gttttattat ttactgaagg tacaataact tcatcaaact ctaaaccttt
    40621 tgacatttta atagaagtta tggatactcc ttttgtaaag ctagaacttt cagaagatat
    40681 taagtgaaca ctatattctt ctcttaacgc atcatataca gctttagcgt cattatctgt
    40741 ctttaatata ataccaagag tcgcattatc actcttttta aattcttcaa tttctatttt
    40801 tatcttattt atttcatctt gctcgttatt acattttaat aaagctggtt cttcaccatg
    40861 tcgttcaata ggctctaatg aagatatgtc ttgaatcttt ttggcgaaat taataatctc
    40921 aaaagtagaa cggtaactct tgttcaacat aactaattcc ccatcattat atagctgttt
    40981 catatcatct agtgtatgag tatgattagg attaaccaat tgtccaaaat ctccaagaat
    41041 agtcttttta cacttaaaaa gtaagtttat tacagcatat tgaataggtg tatagtcctg
    41101 catttcatca ataacaatat gccttataat cttatcttct tgtatacctt catatgcagc
    41161 atgaatatat ataaatgggt atacatctga ccattcaagt gttttctttt ctgccatcac
    41221 aaacatgttt gatatattca tttgtttaaa gaaatcctta tatagtgtaa gagtattttt
    41281 tattgtcaac attccattca aacttttaag tatacttttt acctttggta aatcttcttc
    41341 cataatatta tctgattcaa atttataatg aatgtcttca gctaccttct ctaatctctt
    41401 cttaactgga tatctgttat atgcctcaaa tctactctgt atccagtcac ttttagctgt
    41461 aaaacttcca aaagtgtagt cttttggaat aaaaatctta tttggcattt gttttatata
    41521 atcatcaata agttttacaa aatcaagtgt agatttaaaa cgcactcttt cagaccattt
    41581 agcatcattt atttctaatg ggtctttctc actttcaaaa ttaataactc tatctaattg
    41641 tacctctgct atattttcaa aacttaattc acataatggc tcttctccaa gctcaggaag
    41701 gacattggaa atatagtctc caaagacttt attaggtgat aggataatta cattatttgc
    41761 tgatatttta tctttaaatc gataaagaag aaaagcaatt ctgtgtaatg ctatagatgt
    41821 tttcccagaa ccagctaccc cttgaataat cagtgtatca gctttatcat ttctaataat
    41881 ttgattttgt tccttttgta ttgttgaaat gattgacttc attttttcat ctgaagtgtg
    41941 actaagttct ttttgaagaa tatcatcttg tatattaatg gaactttcta gtgcatattc
    42001 taattttcca tttttaattt taaattgtcg ttttcttgtt atttctccat ccacccatcc
    42061 tattggagca tcatatccag cttttccgat ttcataatca taaaacatac tggcaattgg
    42121 agaacgccag tcaagtatta taagctcatc ttcataatca aatgcaaatc taccaatata
    42181 atacttagat tcatcatcca tatctttcaa cctaaaatct attctagcaa agtatggaga
    42241 atctttgatt ttattaatct tatctcgaac ttttaccata aaagccccat agttatctat
    42301 ttgttttagt ccttgttcat tttgaaacat ttcatgtggg tctatttcac cacgattttt
    42361 taccatatac attttagcat ccatataatc cttatcaagc ttgtcaacaa gattttcagc
    42421 tttcttaaaa gcattgtcta gcttgttatt tatctctatt agatgtgcaa tctcatctgg
    42481 aaaaaaagtt aataactctg catcatattc acacatatta tgtttagaat tagatgcaat
    42541 aatatcgcaa tttactactt gtttattttc agacatttaa atcctccttt gttaattaag
    42601 tacttacact gaatcattat acttaattgt gttttggagt ttttaatcat tcttgctatt
    42661 taacttaaag gaagaataaa tttttgagta ggatatccaa attccacaag caattctgct
    42721 tctgcaaagc ccaacttctt tatcatatct ctatgtcctg tatcagcttt gtcaccctct
    42781 cgaaaggtag taattgaaat atctgtatct atcaagaaat cttcaataac tttcttcaaa
    42841 aacacttgtg caatgtctga attacgatac tgtggatgta ttccaaaaaa atcaatactt
    42901 ctagtttctc tattaaacac catatttgca atagcgacat tattaagttt taatattaaa
    42961 gctctttctt ctaaaatata ctgttctaat tgatatatat attcttcctc ttgcaaattg
    43021 ggaaatccat ctatgactaa tctcactaaa ttcatccata atggtatgtc agacatactt
    43081 gcaagtttaa tttctttttc caactctagt acattctttg aaaataggtt atcttttttc
    43141 aaaacaaatc ttagctgcaa aggataaaat tcttgttctt tcctatattt atttggagat
    43201 tttttataca tagctttaaa tatagaagtg aatgcctgtt gactttcata tccagctatc
    43261 aatgcaattt caataattgg tttctttgaa aataccaata attttgctgc ttctgtcaac
    43321 tttctccgct ttatgtaatc atgcattgtt aggccaacag aagttgtgaa agttctatgc
    43381 aggtgatatt ttgagtaatg aacagcatta gcaactatat ctaaatccag cttttctgat
    43441 aaatgctcct ctatatagtc aatagcagtt atgacattaa ttactttgta agacattgca
    43501 gcttccctcc ttttatccat gcaacattat atcaattatt acttagttat atttaatgaa
    43561 tcttgctatt ttataaacaa atttgttgtt taagttttca attcataaat ttaatatatt
    43621 actttttact ttctgattaa agactctaat cctttaattt tcaataactt ttcatttagt
    43681 aaatctaaaa agttatttat tattttcttc ttattagatt ccttaattaa atgataaata
    43741 tttatattat acgactaaaa acagcttttt acaattattt aatatgatta caaacttacc
    43801 ctatcttaaa gtttaatggg gtcctgttat gaatcttttt attttaattt aagctcttca
    43861 tcagtttatt ttatatattg atgtatttga tttcatacaa aaaagagtgc ttcatgaact
    43921 aaaaagttca tttttaaaca ctctctttta tgtaaataaa tttctattct attttgaaat
    43981 attataattt gacttataat ctattaatac caaaaataat aaattgtacg tctaagaata
    44041 gattctaatt agttgctcag tagtaaaatc tgtttttaac taatcctgct ttgtatgttg
    44101 aacatattct ataattgtac catctggatg tctcaatgtc atattatatc ctgtaggaac
    44161 ttttttctta tctcttacta caactgctcc attatccaat agataacttc tccattcatc
    44221 aacagaatca accataaaag tagattttgt ttctataaat ggtttaagag catcatcaga
    44281 accagataat agtaaaacat ttcctatttg cgctaattcc aacccaactt ctttatagga
    44341 aaaacgcaaa gaacattttt cttttaaaag attctcataa aaaggtatgg ctttttctat
    44401 atcttgaata taaaaacgtg ttaatatatc atatattttc atattttccc cctatatatc
    44461 tatataatat tacctctaaa ttataccact ctacaaatta tataacaata aattaagcat
    44521 agccttttga gcatcttaag tagaccttaa caatatattg gggttacatt agaatacata
    44581 aattaaatca ccttgatatt atttaggata aaatttatgt agtagcagta aatgttcttc
    44641 taattatcaa ctaacaaacg ataatcttta actttctata taatgggtaa catcattttc
    44701 gtaagatatt cactagctgg acgattgata tcatttaaat actggtaata tatatcgcca
    44761 tgaagtttat atccattgct atttgcccat gaaaataagt cttctaaagt agggtcttgt
    44821 aattcataag gtcctaaatc aattgcagtt attattttct gtgcaggaac aatcttttgt
    44881 agaatctcat ttttcccatc tatataagtg gctacaggaa accctacttc aacatccaat
    44941 tttactaaat ccatgttatg aaaacataca atcggtgctc ctgaagataa aattcctctc
    45001 tcctccagat atttagaaat tttttcaaaa ctttgttttg aaaactctga aaattctacc
    45061 ataaagtcaa ttgtttttcg tattgccaaa aaacagtact cagattgttc agctattgtt
    45121 atattggata ttctagtcat tttattcctc cattacttta ttttcatttc taatcttttt
    45181 ataccaaatt atacgaaaat tcactgtgta actaattatt tcatatgaat ataaaacata
    45241 acctccttcc atttcttata tcgaaagtat agcaaaaata atctcatcat tcttgtcaca
    45301 cagtgctctt ataatcagaa catacttaaa agacttgcct aatttttagg caagtctaaa
    45361 agaaatcttt ttctataaaa aatttataca aacaattaaa atatcaaaat atagactaat
    45421 ataaaactat aaatttacta atctgaacct cccatggcta aagtcacaag attactgatt
    45481 tatagaattt cgcacactaa aatatgtctt actaattccc cacaggctac ccccctagtt
    45541 cctacgttct tacataagtt atctattatc aaacatcaaa atttaaacaa gtttcaggtt
    45601 catcttctat tcttgctatt ttaaacttac atgcaattaa tactaaagat actattggat
    45661 ttataatatt taataaagcg tatggaccat atgttagtgc agatacacct aatgttgctg
    45721 ttatatacgc cccgcatgta ttccatggta ctaatggaga agttaaagtt ccactatctt
    45781 ctaaagctct cgacaacatc ttaggatgta tacctctctt tttatattcc ttattataca
    45841 ttcttcctgg tataactata gatagatatt gttcacctgc tattatattg gtaattgtac
    45901 aagttaccat agtacataat actgttccaa atacaccctt tgctaatctt agtaaagaag
    45961 atgctactac ttcaagcata cctgtttttt ctaaaatacc tccaagagat aaagcgcata
    46021 ttgttaagga tacagtggac atcatattca taatcccacc tttagataat aaagcatcta
    46081 cctcctgtat ttgtgttgac gaaacatatc cattttgtgc tgcatctaat atcgtattca
    46141 tagattctcc ttgaaaaact actgcaaaca atactcccaa tacagcacct actataagcc
    46201 ctggtatagc aggaacttta aatacaacca atccaattac tattgctgga gctaataata
    46261 atactggtga taaagtatta aatgtagaat ctaatgtaga cctaattaat tctatttgtt
    46321 ttatatctag tgcttgacct gaatacttca ttccaattac accataaatt actaaacaaa
    46381 ttaataatga tggtatagtc gaatatagca tatattttat atgtgtaaac aaatctgttc
    46441 ctgccattgc tggagctaag ttagtcgttt cagataaagg tgatagttta tctccaaagt
    46501 aagctccaga tattatagaa cctacgatta atccaattgg catacctaaa ctttgaccaa
    46561 ttcctaaaag agctactcct actgtaccca ttgtgctcca agatgaacct gtcgcaagag
    46621 atactattgc acagactaat gttgatgcta ctaaaaaaat atttggtgat aaaattttta
    46681 gtccccagta aatcatacat ggaactactc cacttactat ccaagttcct ataagtacgc
    46741 ctattataat tatgattaag attgcttgca ttgccatctt tatactatta aacatactta
    46801 attctaactc tttccattta aatccaagtc tatatacagc cacaagtcct gctactgctg
    46861 ctgctcctat tagtggtaca tgaggtgatg aaccatacac taatactcct agacttaaaa
    46921 ataatatcaa acaagtaatt ggtatcattg catcaaacag acttgctttt ctacattctc
    46981 gctctttcat aaaatagttt cctcctaatt atttaaacac tacactataa ttttttttga
    47041 aacatatttg ctcgtactag atgacatgat acccccataa gttaataaaa attctaaatt
    47101 gtcacctaat ttataatcat attttgtgtt acttacatct agtatcatgt ggtctgaact
    47161 tgcccctaat atttttatat cagtgtctat tggaattaaa gagtcaatat ttatatcttg
    47221 tttacctata gctatgattg ccctttttaa gattccctta tcttcataaa caggtttatt
    47281 tctaaaggca tcaactccta tttctccaat aggaactgat ggtttttctt tacattcaac
    47341 tacttggcat attaatttaa atgcatcttg atatgttcca tttatatttt caccgtatgc
    47401 cgtttctcta ccaagtagaa tggactcgcc tattctcaaa ttagtaatac cttctggcat
    47461 attattattt atcaacagat gaatagaact tgaattacct ccagatacta tttctagatt
    47521 tattccaaat ttttcttcca ttctttttgc tatacttacc agtctgccta tattttccct
    47581 agatggtata atagctccat aacaggttaa gtttgtagct ataccaatta tttcaatatt
    47641 ttttaatctt actatctctc ttacattatt aaaaaagtct tcttcataaa agtatccttc
    47701 tcgtaaatcg cctaagtcaa acataagaac tatcttatga ataatatttt tactttcaca
    47761 taattcattt aatttcttta ttgttcctaa ttctgagtta aaactaatat cgcaataatt
    47821 gataacttct tctaattcac ttaacattgg gattctaatc aatatcttag gtagatttat
    47881 atcttgtaaa ttttttaagt tttgtattct tgaatctgct atatggtcta ttccttcact
    47941 acatatttct tctactattt ctttttgtgc acaaaatgat ttagtaacaa aagatatctt
    48001 aatatttctt tcatgacaca tctgcgaaat taattttgca ttatgcttta acttttctat
    48061 atctatttct aacctaggat acataatctt tcctcctcaa aattcaaact ttttaacagg
    48121 agcttaagag atgcttcttc atatttttta gataaaacac ccatagatgc cataatatag
    48181 tcattatcta ttaatatctt tgttttttcc tttggaaata gtaattcatt atttttattg
    48241 tatttgagca tattatctaa tatttcaatt ctactaggga gcctagtaag tgctccccct
    48301 gtaccaacta tatatcttac ttctgttaaa tctttccctt ctgcaatttt gacttttcca
    48361 gttgttccat agatatttct tatttttcct gaatgtctta gcatagcttt aacaacagct
    48421 tcttttgtca atctttcaac aaataatatt tcttcttttg attttggtat tggaggataa
    48481 ttctctatta ctgcatcaat atctatagat agttcacttt gtaagttttc tttacctata
    48541 acttctacta tattcttcat atttacatat acacctaaat ccccctcaac acttctcttt
    48601 gcagtaggtt caggattaac caaaatttta ttaatatagt cacttccatc tgtaacagaa
    48661 tgtacatcag tagttgctcc acctacatcc agagttaata aatctccaat atttttatat
    48721 aaaagtttag aagcttccat tacagctcct ggggttggag taatattttc attaaccatt
    48781 tctttaatgt atttcattcc tggtgctgta gttatgtgtt cttcaaatac actttgaatg
    48841 attcttcttg taggttctat attcaataaa tctattttag ggtatacatt ttcagttata
    48901 taaagcttat aattagtatc ttcaaatatt aaccttattt cctcttgatt ttcaacatta
    48961 ccagcatata taactggtat gcctagattc atagaagcta tcatttctgc attataaata
    49021 gctgtatctc tttctccata atcgactcca ccagctatta aaattatgtt tgggtttatt
    49081 tcttttattt tatttaaatc agttctttta attctacctg atgttatttg acgaataaca
    49141 gctccagccc ctaaagctgc ttctttagct gcttttactg tcatatcata gactaatcca
    49201 tgaacagtca tttttaatcc accagctgca ctagaagttg caaacatatc attatacttt
    49261 atatcatcta cagataattt attagctaaa tctttaatag cacctgataa gccatttctt
    49321 acatcgccac cttcaaacac tgttgttgga gcttgacctt gacccaaaaa tataggattg
    49381 ttagtattaa tatcgtggaa agcgtttacc actgtagtag tacttccaat ttcagctacc
    49441 aacacatcta tttttaaaga aaccttgcta ttttccatta tttattactc atttctcttc
    49501 ttttttcaac taagaaagtg gctacatgta tgcctttgct gtttcttcca aatccagcat
    49561 ctatgccttg ttcaacagct atttcaggtg ttacttgtgt tcctccacac gcaatcatta
    49621 ctttatctct tatacctttt tctacacaat aatcgtgtaa ttttttcatg tttttatagt
    49681 gaatatcatc atgacttatt attgtagatg caagtattgc atctgcattc agttcaatcg
    49741 ctgcatctac caatttttct acaggtacag aagtccctag atagtggcat tctatgccat
    49801 atttttcaat acctccatgt tttatatcta ttatttctct taaaccaact gaatgttcat
    49861 cttctccaac cgtagctgaa actattttca taggtttttc ttctatatct gctcttattt
    49921 cttcatcagt tagtacctta ggttcttctg gtattactaa attgtttaaa tctatatcaa
    49981 acggaactct tcctttcact tctattctag tcccttcaga ttcatgcata acttctctac
    50041 ttatgacctc aatttcttgt aagttcattt ttttgacaaa ttccaaagct gcaaactctg
    50101 ctgttctttt atcagtagga agcatcattg ttatcattac aattccatct gcacaccatt
    50161 ccatttcagg cttaatttta gttccatttc tgtactcata tgattctttt agtctatttt
    50221 ctacattatc aaaatcgtca agctcatcaa tatatattat tttgtctgga ttttcaaatg
    50281 tacatccatc tattaacatt gatggattat ctatagcatt ttcatcatat tgagctacat
    50341 tattgtatcc aaagtgagca gttacaggtg ctaaataatc ttcttctctt tcataaacag
    50401 taccttcacc aactccacct tttatttttc tagatattcc atcaccattt ctttctggat
    50461 aatttccaga atcaacaaaa aatccttgtt ccacagattc aaaatatcca ccaacttcag
    50521 atatttcttc catatataat acagctcttt ctttaagttc tcttgctttg tctcttaatt
    50581 caccgacatc tttaagttct atcatatcca taagcccatc cataccaact aaagcttgct
    50641 ttgctgtatc acatgcttct atattgtata tatgccaagg tacatttcta ccttcatcag
    50701 gtgttatagt tgattgtatg tcagctctag ttaatactga tgttaataag tttaatacat
    50761 gagtaactgt tgcttctcta gttgatgatt ccatatattt agtattcatt tgagctctca
    50821 tcttataatc actaaataac tctctaagtg caaccgcgta aggtaagtct atttttaaac
    50881 atggtgctgg aggtgctgtt ggtggaactg ttgataagca tatattactt ttatctatac
    50941 ctattttttc tgagaatata gagtttaaag cgtgttgaac cataagttca ggcattacct
    51001 tccatgcttc tctagcagta gcattagcat tatgtgctcc atcaatttga gctatattgg
    51061 caaaagccat tattttcttt gcctcacaag catctacaaa agaccttacc atgtttatat
    51121 tcctatacag aacattatat tgtgggtctt gatgtgctcc attgactcct tcctcagcaa
    51181 acattactgc tacttcagga ccagcaacac cacttacata tgaatggtaa ttaataggtc
    51241 taccaacttc ttcttctata aaatcaagag ccttcctttg agctcttact tgttttcttg
    51301 ttattggaac tccacctata ccttgtggag ttccttctat aagcccatca aaatgtgatt
    51361 gtccagcagt tctgataacc attagatggt cagctccatg atgagctgcc attctcattc
    51421 ttcttatatc atcctcaaat ctaccagaag ctatttcagt agtaataact ggacttggtt
    51481 gagggtctat attattgaaa tacttagatg atggtagccc tacactcttc tttaaaggtt
    51541 ttgtacagtc tttatattta aaaggtccca tttcaagatt ttccaagtgt tctctccaaa
    51601 cccaacctct tcgttttggt ttatatttat ccaaatcttt taatatatag tctatatcca
    51661 attttttatt ttcctttaac atgattctac cccttctcta aataaacatg taacttcatc
    51721 ccaatattca cccttaacta ataacaaacc tgcttctcta accgttatat ttttagattt
    51781 agccaattta taaactacat gccctgctcc tttacctata agacctcttt ccataacccc
    51841 ttctaaaata ggttttactt caagagaaga aaatcccatt ctcaacaaga cagacctttc
    51901 tattgatggt gttgtattct ttttaccaag ttctatcatt ggttctacta tctgggtagc
    51961 taaactccaa aacctttctt ttaactcatc atcactcagt tcttgaagat gttttcttct
    52021 aacttcaaaa tcatcctctc tttttttcat gaacattcct ccattcgtat tgatttaatc
    52081 gaattttctg aatttttatt ttatattaaa ttaaaaaaca ataattacta taaattattt
    52141 aatacatcaa caacaaaatc tcttgaactt tttacttctt ccattaaaaa ttctatatct
    52201 cgattactta gcttatcaat attatcaatg tgattattag atatacaatt ttttataaat
    52261 gactttctta ttttattcat atcaatatca acacatttca ataattctgg tctttctggt
    52321 aatattaagt ttactcctgc aatttcttct cttggatttc caaattttat atctattcca
    52381 ttttctcttg caaaggtaag ttgtggacat atatgtttac ctgcacccgt atattctgtt
    52441 tcttgtacca ctatagcttt atcctcatct agctcttgag ctaaagagaa tgctgctgct
    52501 aaagatgtat ttcctgctgg acctctttct ataccttcta gctgtgccaa taattcagtt
    52561 gtataaaaaa cttctccttg ttttactaat acatatctat ccatatatct aagtggtcta
    52621 gctgcagacc taggtacatc tgacctatca ggccatgtac agtatggtat cccaaaacct
    52681 gtatgtcctg ttgtaaatga ttttttatta aattgattat cactagccat gtgtaatcct
    52741 tttaaatcta cacttgctcc tactacttta gtattaatag aaccagcttt tatcaatcct
    52801 cttgcagttc cagtaagatt accaccacct gcatttgtac aaacaactat atcagggtct
    52861 tttttatact tttctctaaa ttggatagct aactcataac ctaaagtttc tacacctgca
    52921 attccaaatg gtgaatataa tgatgcgtta aaatagcctg tttcttctaa taaaactaag
    52981 aacgtataaa ataactctgg tccaacagtt aactgaacaa cttctgctcc atatgcctca
    53041 cattttcgcg ctttttctat gatttcaggt tgacctacac cattagaatc ataacattct
    53101 tgaactatta tacattttaa tccctgcatt gccgcttgtg atgctacagc tgcaccatag
    53161 ttaccactag tagctgctat tacaccttta tatcccattt gtttggcatg atatactgca
    53221 gtagctgctc ttcttgcttt aaaactacca gatgcattca ttgcttcatc ttttacaaat
    53281 attctagctc ctttaccttc tttagcacat tttctagcta atttagtcaa atttttaagc
    53341 tctattaaag gagtatttcc aacagcatac tgtgattgaa ttgcttcaat atcttgtaat
    53401 gtatatcctg tttcactcat cattttctca taatcaaacc ctattccctc ttgttcaaaa
    53461 gaagaataat ccaatccgat tgcatttttc attatttcgt tatttcttcc cataacagct
    53521 tgataactca tatctttcat tgatgttgaa ttattcatta tatacacctc caaataatat
    53581 ttctttagct tggattccta tttggagtaa ctctggtata catttaccat aatcatgagt
    53641 ataatatgga tttattttaa gtaaattacc ttttacaagt ctgcctgtaa ttgttttaac
    53701 ttctactaag tcgcctatag aagcatcact ttgtataaaa cctttaaccc acatttctaa
    53761 ggatactttc ttagtatcct ctggaacttg tggagctctt tcttctggag tcaatactat
    53821 attgtgtata ataacccaat cattaatttt agcatccatt ttttcatccc cttattaatt
    53881 gactattgcc ttactaatta atttactgtt attttaaatt aaacattatt ttaccaatta
    53941 gctaactgtt atcttactaa ttagctagtt gctatcttac taattagcct ttgattcttc
    54001 ttccaattca acttctatct gatttcttat atctcccatt attgctctag gtactggtat
    54061 atccaacata gttttaagac ctggtctagc atttattatt aatggaatag agtttactat
    54121 cattgcataa gtacctactc ctccaggtat ttcaggattt atttgtaaat caatgtttgg
    54181 tatacctttt attgatacat agtctcctgt tttaatacct tctgcttctg gtattatctg
    54241 ttgtggatgc tccatttcaa ttaaaacttc tccatttaca tatccatatc cacattgtct
    54301 acaaccagca acatttccag ccaatgcctc tccgtactta gattttctat ctactgtagt
    54361 cattattggc tcttttgttt gttctatctt atctaatttc caacctaatc catcagctat
    54421 catatttatt gattctacaa aacctacatg acctgctata gtcttatctt ctacaccttt
    54481 tataaattct tcccttgtta ctccaacacc ttgttccacc ataacagact ttccaaatgg
    54541 agataaatca tttactctct tagcttttat actagtaact tcttcacaag tacctgaaag
    54601 tgccaatact agtaaatcta atacaaatcc tggatttatt cctgttccaa gtatacttac
    54661 tccattttcc tttgcaactt catccatttt tttagctaaa tctgcatcat cagcttgtgg
    54721 atatgccatt tgttctgctg ttgatataac atttattttt ctatttaata agaaatttat
    54781 tttatcaaaa gcctttttag taaatgaatc agttgctaac aaaacaacat ctacactttt
    54841 ttctctaaaa acttcttcat aatttttatt gataataact tctggtcttt ctcctctttc
    54901 aattcctaaa acatcataca tacttttccc tgaagtactt ctactacaaa cagatactat
    54961 ttctatacct tctttcttta gaatcatatt agccatacct attcccatag ctccaaatcc
    55021 ccatattcct actcttactt ttctcatata tacaccctcc taaaaattta tatattctca
    55081 ataattagtt aaagcataaa tcgtgccaac tttaaaatta aagtaaatat ttattggaac
    55141 gttgaaacta taattatttt attatgtatt ttcataaata aaaaataata tttcttatga
    55201 gaaataaaaa aaagagcaaa atattttgct cttttataat aattttgctc ctctattttg
    55261 cttttttatt ttactttgat tttgtttttt tatcttgctt atttataata ttttgatatt
    55321 atattttttt attttatact gtaaattttg cctattcata cctaaaattg atgcagcttt
    55381 agttatattg taagatgcct tttctaatga ttcatatata tacttttctt ctatctcact
    55441 gataatctta ctaagtgttt tgtcctccaa atcgtttaaa gaataattgt tactctcttt
    55501 attctcttta agagtgtaat agtcgtttag attaatcata cttgtttcta tttgtacctc
    55561 atcttccatc atactcatag tacaataaat tatgttttct agctctctta tgttaccttg
    55621 ccaatcgtaa ttttttaaaa ttttaatagc ttcatctgaa attcctacca cttttttatt
    55681 taacttttta ttatactttg aaataaattt tttgcacaat agaggtatgt cctcttttct
    55741 atctctaaga ggaggaattt ttatatgaat tatgttaagc ctataatata aatcttttcg
    55801 cattttatgc tcgtttaaaa ttacctttgg actttcattt gtcgttgcaa taattcttac
    55861 atctataggt atatccttag ttcctccaac ccttcttaca taaccttctt gtaaaaccct
    55921 taatagttta gcctgtaaca taataggcat ggaatttatc tcatccaata acaatgtccc
    55981 tttgttagct tgttcaaata agcctggcct atctattgct cctgtaaatc ctcccttgct
    56041 agttccaaaa agaatacctt cgaatagtga ttccggcaaa gctgcacaat tttgtgctat
    56101 aaatggatag tttctcctat tacttccata gtgaatggac tggctaaata actctttacc
    56161 cgttccagtt tcaccgtata taaatactga agcatcagac ttgcaagctt tcttagattt
    56221 agctattgct tctttaatca aattactttc ccctaaaata tcatcaaatt cataaccctt
    56281 tttttgtcta atactaactt tattgactaa atttttttta taatttctat ttagtaaatt
    56341 ttctatcgtt gtcatattct ttgatatttc aagtgcccct acaaactcgc ctttaatatc
    56401 aattggtatt gtagtattaa ttgctgttat ctcttttcca tttttatcca gatatttttg
    56461 aatattgtct ttgataactt cccttttttc caaaacttct aatagtgtac tcctgtttat
    56521 tttcattttg ttgactatat caaaaaatga attggttaaa atatcgtgtc tgttcatttt
    56581 ttccattttt tgtacagaat tattgtagat tatagttttc ccgtctttat taacaaaatg
    56641 taccccttca tcaatatttt ccaatatttc attcattatt ttaaaaaata tctcttggtt
    56701 caactatatc accccagtac ctaaatatat aaaaacccaa aaaattaata aaatgttgct
    56761 attgtattta
//
LOCUS       DAEMGG010000010        55927 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEMGG010000010 DAEMGG010000000
VERSION     DAEMGG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.010"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            100..2289
                     /locus_tag="KPK24_000631"
     CDS             100..2289
                     /locus_tag="KPK24_000631"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG2298339.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            2357..3124
                     /locus_tag="KPK24_000632"
     CDS             2357..3124
                     /locus_tag="KPK24_000632"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG2298340.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            3182..4198
                     /locus_tag="KPK24_000633"
     CDS             3182..4198
                     /locus_tag="KPK24_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG2298341.1"
                     /translation="MEYNELLKSARENLNGCCKVCKVCNGIACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            4264..4512
                     /locus_tag="KPK24_000634"
     CDS             4264..4512
                     /locus_tag="KPK24_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298342.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(4688..5185)
                     /locus_tag="KPK24_000635"
     CDS             complement(4688..5185)
                     /locus_tag="KPK24_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG2298343.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5429..6493)
                     /locus_tag="KPK24_000636"
     CDS             complement(5429..6493)
                     /locus_tag="KPK24_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298344.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            complement(6496..7593)
                     /locus_tag="KPK24_000637"
     CDS             complement(6496..7593)
                     /locus_tag="KPK24_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298345.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            complement(7607..8542)
                     /locus_tag="KPK24_000638"
     CDS             complement(7607..8542)
                     /locus_tag="KPK24_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298346.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(8671..9318)
                     /locus_tag="KPK24_000639"
     CDS             complement(8671..9318)
                     /locus_tag="KPK24_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2298347.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            complement(9311..10474)
                     /locus_tag="KPK24_000640"
     CDS             complement(9311..10474)
                     /locus_tag="KPK24_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2298348.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSIALYRLIQESLSNSSRHGKATEIRIFITFNTSNLIITISD
                     NGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            10659..11003
                     /locus_tag="KPK24_000641"
     CDS             10659..11003
                     /locus_tag="KPK24_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG2298349.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTSIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            11034..12635
                     /locus_tag="KPK24_000642"
     CDS             11034..12635
                     /locus_tag="KPK24_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG2298350.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            12654..13754
                     /gene="dprA"
                     /locus_tag="KPK24_000643"
     CDS             12654..13754
                     /gene="dprA"
                     /locus_tag="KPK24_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG2298351.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            13787..15874
                     /gene="topA"
                     /locus_tag="KPK24_000644"
     CDS             13787..15874
                     /gene="topA"
                     /locus_tag="KPK24_000644"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG2298352.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            16039..16824
                     /gene="codY"
                     /locus_tag="KPK24_000645"
     CDS             16039..16824
                     /gene="codY"
                     /locus_tag="KPK24_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG2298353.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            16931..18196
                     /locus_tag="KPK24_000646"
     CDS             16931..18196
                     /locus_tag="KPK24_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG2298354.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            18227..18997
                     /locus_tag="KPK24_000647"
     CDS             18227..18997
                     /locus_tag="KPK24_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2298355.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            19106..19552
                     /locus_tag="KPK24_000648"
     CDS             19106..19552
                     /locus_tag="KPK24_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG2298356.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            19570..20763
                     /gene="nifS"
                     /locus_tag="KPK24_000649"
     CDS             19570..20763
                     /gene="nifS"
                     /locus_tag="KPK24_000649"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG2298357.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            20817..21257
                     /gene="nifU"
                     /locus_tag="KPK24_000650"
     CDS             20817..21257
                     /gene="nifU"
                     /locus_tag="KPK24_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG2298358.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            21546..22625
                     /gene="mnmA"
                     /locus_tag="KPK24_000651"
     CDS             21546..22625
                     /gene="mnmA"
                     /locus_tag="KPK24_000651"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG2298359.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    22768..23006
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            23074..25713
                     /gene="alaS"
                     /locus_tag="KPK24_000652"
     CDS             23074..25713
                     /gene="alaS"
                     /locus_tag="KPK24_000652"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG2298360.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            25738..25995
                     /locus_tag="KPK24_000653"
     CDS             25738..25995
                     /locus_tag="KPK24_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG2298361.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            26005..28176
                     /locus_tag="KPK24_000654"
     CDS             26005..28176
                     /locus_tag="KPK24_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298362.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            28382..28807
                     /gene="ruvX"
                     /locus_tag="KPK24_000655"
     CDS             28382..28807
                     /gene="ruvX"
                     /locus_tag="KPK24_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG2298363.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            28885..29172
                     /locus_tag="KPK24_000656"
     CDS             28885..29172
                     /locus_tag="KPK24_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG2298364.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            29454..29912
                     /locus_tag="KPK24_000657"
     CDS             29454..29912
                     /locus_tag="KPK24_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG2298365.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(30029..30766)
                     /locus_tag="KPK24_000658"
     CDS             complement(30029..30766)
                     /locus_tag="KPK24_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG2298366.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFIAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            30983..32659
                     /locus_tag="KPK24_000659"
     CDS             30983..32659
                     /locus_tag="KPK24_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG2298367.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            32865..33065
                     /locus_tag="KPK24_000660"
     CDS             32865..33065
                     /locus_tag="KPK24_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG2298368.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            33119..34282
                     /locus_tag="KPK24_000661"
     CDS             33119..34282
                     /locus_tag="KPK24_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2298369.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEIISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(34493..34915)
                     /locus_tag="KPK24_000662"
     CDS             complement(34493..34915)
                     /locus_tag="KPK24_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG2298370.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(34936..35772)
                     /locus_tag="KPK24_000663"
     CDS             complement(34936..35772)
                     /locus_tag="KPK24_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2298371.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFGSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            36070..36666
                     /locus_tag="KPK24_000664"
     CDS             36070..36666
                     /locus_tag="KPK24_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG2298372.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            36696..37415
                     /locus_tag="KPK24_000665"
     CDS             36696..37415
                     /locus_tag="KPK24_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG2298373.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            37417..37956
                     /gene="scpB"
                     /locus_tag="KPK24_000666"
     CDS             37417..37956
                     /gene="scpB"
                     /locus_tag="KPK24_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG2298374.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            38117..38746
                     /locus_tag="KPK24_000667"
     CDS             38117..38746
                     /locus_tag="KPK24_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG2298375.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            38700..39128
                     /gene="ytfJ"
                     /locus_tag="KPK24_000668"
     CDS             38700..39128
                     /gene="ytfJ"
                     /locus_tag="KPK24_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG2298376.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            39376..39816
                     /locus_tag="KPK24_000669"
     CDS             39376..39816
                     /locus_tag="KPK24_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2298377.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            39832..41283
                     /locus_tag="KPK24_000670"
     CDS             39832..41283
                     /locus_tag="KPK24_000670"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG2298378.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(41374..42042)
                     /locus_tag="KPK24_000671"
     CDS             complement(41374..42042)
                     /locus_tag="KPK24_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG2298379.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFILSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            42311..42451
                     /locus_tag="KPK24_000672"
     CDS             42311..42451
                     /locus_tag="KPK24_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298380.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(42626..42946)
                     /locus_tag="KPK24_000673"
     CDS             complement(42626..42946)
                     /locus_tag="KPK24_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG2298381.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(43066..43803)
                     /locus_tag="KPK24_000674"
     CDS             complement(43066..43803)
                     /locus_tag="KPK24_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG2298382.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            44018..45841
                     /gene="acd"
                     /locus_tag="KPK24_000675"
     CDS             44018..45841
                     /gene="acd"
                     /locus_tag="KPK24_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG2298383.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            46056..50354
                     /locus_tag="KPK24_000676"
     CDS             46056..50354
                     /locus_tag="KPK24_000676"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG2298384.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            50558..51019
                     /locus_tag="KPK24_000677"
     CDS             50558..51019
                     /locus_tag="KPK24_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG2298385.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            51049..52203
                     /gene="nusA"
                     /locus_tag="KPK24_000678"
     CDS             51049..52203
                     /gene="nusA"
                     /locus_tag="KPK24_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG2298386.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            52210..52491
                     /locus_tag="KPK24_000679"
     CDS             52210..52491
                     /locus_tag="KPK24_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG2298387.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            52481..52792
                     /locus_tag="KPK24_000680"
     CDS             52481..52792
                     /locus_tag="KPK24_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG2298388.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            52814..54754
                     /gene="infB"
                     /locus_tag="KPK24_000681"
     CDS             52814..54754
                     /gene="infB"
                     /locus_tag="KPK24_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG2298389.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            54797..55177
                     /gene="rbfA"
                     /locus_tag="KPK24_000682"
     CDS             54797..55177
                     /gene="rbfA"
                     /locus_tag="KPK24_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG2298390.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            55158..55835
                     /locus_tag="KPK24_000683"
                     /pseudo
     CDS             55158..55835
                     /locus_tag="KPK24_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT        22421 a         6034 c         9839 g        17633 t
ORIGIN      
        1 agataattaa tatgaaaata tacaaatata taaaaagttt gtttagagtt atagttaatg
       61 tgtgtattaa tttaatacta atttattgga ggtttaaaaa tgaaatacat aataaaaaga
      121 gatggtacaa aagaactttt tagtaaaagt aagattgaag atgctgtcta taaagctagt
      181 ataaatagtg aaggtggagt agatagagag ttggcatatg atatatcaag aaaaatagct
      241 aatgactata attctatgga aaaatgctta tccgtagaag aaatacaaga tatagttgaa
      301 gtactactta tggaatcaaa tagaaaagat atagcaaaac actacatatt atatagagaa
      361 agacgctctg aaataaggac taaagcttgg gagtttgatg aattacaaaa atcaatatgg
      421 aagaacaagt atagacataa tggtgagagt tttgatgaat ggataaagag aatttcaaat
      481 aatagttcaa gaatatcaaa actaataaga caaaaaagat ttctatttgc tggaagaatt
      541 ttagcaaata gaggattaca gaaagaggga aaaaaagtaa catattctaa ttgttatgta
      601 atagaaccac caaaagatag catagaagat atatttgata ctgcaaaaaa acttgctaga
      661 acttttagtt atggtggagg agttggaatt gatatttcaa agctaagacc aaaaggagcg
      721 aaagttaaca atgctgcaaa acatacgact ggagcagtct cttttatgga actttacagt
      781 atgacaactg gcttaattgg tcaaagaggg agaagaggag ctcttatgat ttctatggat
      841 gtaaatcatc cagagataga agactttata gatataaaaa cagatttgga gaaggttact
      901 aaagcaaata tttctgtaag agtaacagat gaatttatgc aagctgtaag agaaaaaaag
      961 ttatataatt gtacttttga agttgaaact actggagaaa aaatagttaa gatagtaaat
     1021 gcttatgatt tatttaataa atttgtgaaa aataactggg attatgcaga accaggtata
     1081 ttgttttggg ataaaataaa aacatataat tttatgagtg aagataagaa cttttgcttt
     1141 tcaggagtaa atccttgtgc agaagaacca cttccaagtg gaggaagttg tctacttggc
     1201 tcaataaact tgtcagagtt tataatacaa ccatttggtg aacatgctac ttttgatatt
     1261 aataaattaa aatcttgcgt tagagaagct gtagtagctt taaatgatgt cttagatgag
     1321 ggattgaact tgcatccatt acaagaacaa agagatagtg tacataagta tagacagata
     1381 ggtttaggtg taatgggtat agctgatatg cttataaaaa tgggaataag atatggttct
     1441 gatgaatctt tagaactatg tgatttaatt ggtaagacta tgttaaatga agctgtgaag
     1501 caatcagcat taattgctaa agaatatgga acatttagtg agtataaata tgaaaatatt
     1561 tctaaatcta agttttttat agaaaatcta aatgaagaca ctaaggaaat ggttaaaaag
     1621 tatgggctta gaaattcaca acttttaacc atacctccta caggttccat atctacaatg
     1681 ataggtatca gtggtggaat agaacctata tttaatcttt catatataag aaaaactgaa
     1741 agcttgcatg atgaggatgt gtattataaa gtatatactc caattgttaa agagtacatg
     1801 gacaagaaag gtattacaga tgaagaatgt ttaccagatt tttttgttac tgctatgaat
     1861 ataggatatg aagaaagaat aaacatgcaa aaagtatggc aaaagtatat tgatgcttct
     1921 atatcatcta caataaatat accttttgaa actacagttg aagatgtttt caatatatat
     1981 attaaggcgt gggaggctgg tcttaaagga gtaacaatat ttagagatgg atgtaaaagg
     2041 agtggaattt taataaatga aaaaaaggaa gaaaataata aagaagataa aaaagaaaat
     2101 agtaaaaaag aatattataa aaataataaa aaagaaaaag ataataaaga tgaaaaagcc
     2161 aaagaaataa taaataaaaa ttttgaagaa gatgaaaaat ttgtgtgccc tgagtgtgga
     2221 agtgaaggaa ttgcacatac aggtggatgt agtatatgtt taaaatgtgg ctatagtgga
     2281 tgcaattaaa ctctaattaa aaaattttaa tatattaaaa aattaaattt ttaaaaacga
     2341 aagatgaggg aatggtatgc aggataaaag tgtgagggag attaaagaaa taatagagac
     2401 tttagaagta gaaaaatata tggaatatat agagctgtta agagttgatg aaagaaaatc
     2461 tgtacaaggt ctagctataa aactagcaaa aaagctggat aatattagaa aagaagaaga
     2521 aagattggaa actataaata tatttgagaa tgaaggctat gataaaggtt atttatatat
     2581 tggaggaatt gatgaggcgg gaagagggcc acttgctgga cctgttgtag cttctgttgt
     2641 agtatttaaa aaagatacca aaatagaagg tgtaaatgac tctaagaaac tgagtgaggc
     2701 aaaaagagat gaactttttg aagtaataaa agaagaagca ctagattatg gaataggtat
     2761 agtaaataat gaagaaatag atgaatttaa tatattaaat gctacatata tggcaatgaa
     2821 aaaggctata aattgtttaa aaaaagcacc agattattta ttagttgatg cagcaacaat
     2881 accaggtatt gacatatctc aaaatccaat tgtgaaagga gattcaaaat ctatatcaat
     2941 agcagcagca agtatacttg ctaaagttac tagggatagt ataatgtacc aatatgatag
     3001 agtataccca gaatatgggt ttaaatctca caaaggatat ggaactaaag aacactatga
     3061 agctattgaa aaatatggca taactcctat ccacagaaaa agttttttga aaaatatact
     3121 gtaaatataa tatgatataa tatatataat ataaattact atagttaatg ggggattaaa
     3181 gatggaatat aatgaattat taaaaagtgc tagagaaaat ttaaatggat gttgtaaagt
     3241 ttgtaaggtt tgtaatggga ttgcttgtgc aggtgaagtg cctggcatgg gaggtaaagg
     3301 aagtggttct tcttttatag aaaatagcaa gagtttagaa aaagtaaaag taaatatgag
     3361 agtaatacat gatgtatcaa atcctgatac atctatagag atgtttggaa agaaaatgag
     3421 tgcacctata tttgcagctc cagtaacagg aactacttta aatatgggtg gaaaaattaa
     3481 tgaaagagat tacatagaac cagtagttgc aggatgtgct aatagtggta tttatgcaat
     3541 ggttggtgat acggcagtag atgctttttt aatggaaaat ttagatgtag tcaaaaagta
     3601 taatggagct ggaattgtat ttattaaacc ttgggataat gaaaatatta taaaaaagat
     3661 aagattagct gaagaagctg gtgcatttgc agttggagtt gatatagatg cctgtggtct
     3721 agtaacttta tcattacatg gaaaaccagt acttcctaaa aatgttaaac aaataaagga
     3781 gttagtaaag tctactaaac taccatttat attgaaaggt ataatgactg ttgaagatgc
     3841 tttaatggca gtagaagcag gtgtggatgc aatagttgta tcaaatcatg gaggaagagt
     3901 gcttgactgt acaccaggag catgtgaagt tttaccaaaa attgctgatg ctgttaaagg
     3961 taaagttaca atacttgcag atggtggagt gagaactggt ttagacgtac taaaaatgat
     4021 aggtttaggt gcagatgcag tattaatagg aagacctttt gtgactgctt cttttggtgg
     4081 tgcaactgat ggtgtagaaa cttatgtaaa taaacttcaa tcagagctaa gtagttctat
     4141 gatattaact ggatgtcaaa ctataaaaga tattgatgga aaagttatat acaaataaaa
     4201 ttttatacaa aaaagaggaa aaatcatttt aatgtagaat aattgtacaa agggggaatt
     4261 tatatgagta gaaaagctta tgaagaagcc ttagtagaat tagaaaaatt tattgatgaa
     4321 agaaaagaaa taattaaaag tgcggaggac tgtattgata agtacatagt tgacagaacg
     4381 ctaccttttg attacaaaga taaatgtgtg gaatggcaac aagagttact tgatattgca
     4441 gaggcacaag ttttagaagc aaatgaatta agtgtattat tacaagagaa aaaagaactt
     4501 gaagaagatt aaattttatt agctaagagt aaatcagtaa tatgatttac tcttagtttt
     4561 ttttatttga ataaaataca tctatctaat tgagataact ttattaatat tagattaggc
     4621 agtttaaaat aagctaaagt agcatggatg attagtataa aaaataaaac cagtttaaaa
     4681 aactggttta atttgtattc tttgaattta atatatgttt tttcatttcc tctaatacca
     4741 ttgtatagga tttacattga gcagcagcta attctgataa agcaaatttt tgagctttat
     4801 ttaaaaactc cttatctgtg ctacacaatt gttttgaact gtcttgtatc ataagcattt
     4861 tgattaattt tacaatttca aaaacatctc ctgaatgaaa tttatcttgg tacagtttaa
     4921 gtctttcttt tctatcaaaa atccatacac catctgtttt attaatagat tggataaaat
     4981 tatcaacatc atttccatcc ataactggac gtatgccaat tctatctgca tttagaactg
     5041 gaatcgaaat tttaaggtta ctattaaaaa tgacacttaa ttcatagtat ttttgcatct
     5101 catgatttat tttctttgtt acaatatcat ttacagagca ggctccctct tttggataca
     5161 ttacactttc accaatttta tacatatgct agttatacct ccaaattgaa ttttagatat
     5221 tgcaacttag gtacaatgct ttaattatct aatgattaat attataacat aaataacttg
     5281 acaagtcaaa aatattaata tataatataa agtttagact tggtgatgtg tgaaactaaa
     5341 gagaaaaata taagataaaa taatttaata tgttgaaata ttcttttcca attatataaa
     5401 aatagatact aattatattt aataattatt agtttttagc aaaatctctt agtttaagac
     5461 ttcctgcagt tacaaatata attccaatta atattagagc tattatattt attaaaatac
     5521 taccattgtt tttaatagca tctatagtcc aataaaatgg tgtgaacttt gaaagattag
     5581 tcataaagga taatttatcc attgatggta ttagactaga gatagatata gctagaccta
     5641 ttaaagaata aaatgtagat ataagactta tagaactttc atttttaaat actctagcta
     5701 cccaaaatac aaatccagtg gaaacaaaac tatttgctag tacaatcatc aatacattca
     5761 gactcaaatt aaagtcttct ataattgaaa aacataatag agcaaaagtg tatacaattg
     5821 attgaataaa aaatagtcct aaaaatatac tgaaaattat ttttatatct ttattatctg
     5881 tagagataag tctttttagc acatttgatt tttttaatgt aaataaatca gaagcaagat
     5941 gtgccgcatt aatatacata aaatagcata tcaagaagac tactaaaaat gaacctttat
     6001 tcttatctgg tttgttatca attatatttg tctttgtcaa tttatcatct atatttttat
     6061 ccactttcaa ttttaacgat ttagtaataa aagattctat ttgtgattct gcccaaagac
     6121 taccaccacc attttcaatt ttaaagcatt taactacagg tcgttgtaat ttatctatac
     6181 tatttgaaaa atttttatct aaaacgaata cagcagaaac cttattattt tttaatagat
     6241 tcatagcctt atcttgttca tttaatctaa atgaattttc ttttaattta gaactattta
     6301 ttaacttatg ttcatacttt cctgaagagt cagatacaat tgctactgga gaaaagtaca
     6361 ttgattgatt tcctgagttg ttgataaaaa aattaagtga aaaaattagt ataataggaa
     6421 gcataaccat attaattaaa agagtatgat tttttagata tctttttata tttgcaatag
     6481 ttaatctaat cattattaaa cctccctcca tttatatttt tcctttacaa tagcaagtgc
     6541 aattaatatt gtagagataa ttaaacatgt gaaaattaaa tctcctattg aatctatagt
     6601 tttaaatgtt tcataattac taaacaattc tcctattaaa taggtaggag ataaacttgt
     6661 aagcttagta aatgtatcac ttatcatact gaagatacct ccaaaaattg tctgtaaaaa
     6721 gagtaatgca taaactattg ctgtaccgta ttttttacta aaaaagctgg ttacaaaggt
     6781 agatattgaa gtcataaaat aagatgataa agcacaaagt aggagcaata tggcaaaatt
     6841 acctttaaat gttattccaa gtattctaaa gaataaaaca tataataata agaatataaa
     6901 tgaataaatc cataaaccta caaagtcata aattaagaac tgtactctag ttataggcat
     6961 agaataaagt ctttttgaaa gtccctttga ttcacttata taatttgata aaatattgtt
     7021 cattataaaa attattacaa gaaatcctag tgaaactaaa gcaaagtatt cataagagct
     7081 ctgtttaaca ttgttattta ttatagaagt atcaatagaa tttttattaa ataatttaga
     7141 aaaatcttct gaacttattt tctgagaatt atttaaataa aatttttcat ggtaagtatc
     7201 taatatatct tgaagcaata ttgcaatatc atcacgagtt ccaagttttt ctatgtttag
     7261 agtatttgat ttttcattta ataaactact ctcatagcct ttaggtatag taagctttaa
     7321 ttctgcctta gaatcatctt ttttaactgt taatatataa gataaatcat ttttcaaata
     7381 ctttgtcaaa tcatttgata atcttgtgtt atctttgtca tatataacaa tgggggtaga
     7441 ttctgttttt atagggtttt gaaacatgct ttctgtaacc atcccaagaa accaagataa
     7501 aatcagaggc aaagctaaaa aatatattaa attaagtata aaggagcttt ttaaactctt
     7561 tagcatagtt tttacactca tgtataattt cataaaatca cctccattag tcccttaaat
     7621 tttttcctgt tagatttaaa aatacgtctt ctaggctagg ttcttcataa gaaattttca
     7681 ttatttttga attgtttttg tcaataacag agagtatatc tgtaagttgt aattttttat
     7741 ctatatccaa ttctaataat gaattatttt ctaaaacaga tataacacct ttagtatttt
     7801 ttaaatcaaa gataagttgg gcatttatat tttttatttc cataatcaat tttgtatttg
     7861 tagatacgag tgattttaaa taatcattat ctccaaaagc aatttcttta ccttcatcca
     7921 taataaaaat tttagaacaa aggtgttcaa cttcttccat ataatgggaa gtatatatta
     7981 cagtagtttc atgtttttta gatatatctt ttataaaatt gaaaatatga tttcttgatt
     8041 gggggtctac acctacagtt ggttcatcca atattaatat tttaggatga tttaagagtg
     8101 ctattccaat atttaatctc ctttgcatac caccagataa ttttttgacc tttttctttt
     8161 tgacttcagt aagacctgta acttctaatg cttcaatgat tctttcttta agtagttttc
     8221 cctttaaacc gtagaaagct ccaaagtact ctaagttatc aaaaggagta agagtttcta
     8281 taattgctaa ttcttgtgga actaacccta ttatagactt tgcttttata taatcttttg
     8341 ataaatctaa accattgatt tgaatactac cactatttgg taacataaga tttgtaatta
     8401 tatttataag tgtagattta cctgcaccat ttgggccaat aagtccaaat atctcacctt
     8461 ttttaacaga aaagctgatg ttatcaagta ctaatttatc attaaatctt tttgttatat
     8521 tatttactct gactatatcc atagtaatcc ctccaaaata attttgtata agttaaaaag
     8581 ctataatatt ataataaaga attgtgaatt ataattctat tactataagt cactaaaaaa
     8641 agtgacttaa gtcactttat atttttagat ttattctatt acaattttat ttttgaaagc
     8701 aaagattgct agttgagttc tgtcacgaag ttctagttta gctaaaatat tagttatgtt
     8761 attctttatt gtaccttcag ataagaatag ttcttgactt atttctttat tattaagtcc
     8821 atttgcaatt agtctaatta tagatatttc tttttctgtt aaattgtaca tgtcataaag
     8881 atgctcttgt ttaaccgttt tttcagaggt catgattttt tcagctacac ttttatcaag
     8941 cacaatattg ccaaaaaatg aagaacgaat cccttcatgc aatacttcat cagagctatc
     9001 ttttaaaagg tatcctgaag caccaaactt catagcatct tttatatatt catcatcatt
     9061 aaaagttgtt aaaattaata atgttataga attattatat tcctttataa gccttgtacc
     9121 tagaacccca tcacatactt tcattcgtat atccataaga actatgtcta catcattcat
     9181 tttacaaaat tctaaagctt catatccatt agaagcatct cctacaattt ctaaatcatt
     9241 ataagatgat aatataattt taagaccttt tctaattaat ttttcgtcat ctactataag
     9301 aactttaatt ttattcaaaa aaatcacccc ctgaaaattt aggaaaagaa gctctaattg
     9361 tgaaaccatt attagaatta tcaaattcaa ctttaccatt taattcatat attctctcat
     9421 tgatactatt aagtccattt ccttttttta tattgccaca tcctatacca ttatcactaa
     9481 tagtaattat aagatttgaa gtattgaatg taataaaaat tctgatttca gttgcctttc
     9541 catgcctaga tgagttggaa agggattcct gtatgagtct atataaagct atagattgat
     9601 ttctggataa gttccaagta ttttttgaaa tagttaattt aacattaata tttgtaagct
     9661 taataaagct tttaactaat tctttgattt taaataaact ttggtaattt tcatattcaa
     9721 caggttttaa ctcagatata gcccttctta catcttgaaa actttcagtt acaaattctc
     9781 taagttcatg tactaagtca taaattagag gtttgtcttt taagagtttt tctatagcat
     9841 ttaattgtat tattgttgta gaaagagaat gtcctacact atcatgtatt tctcttgata
     9901 ttcgatttct ttcttttaaa attgctaatt cttctataga acttgcataa gtttccaaat
     9961 cagcattaac tttttttaat tcatctttag aaattctaag cttatcataa agattttgac
    10021 tgtataattt tgataaattc tcattttcta tatatataga aagtacagtt ataattagca
    10081 atatagagct tgactccagt gccaaatata tatttttatc taaactaata ataagagaag
    10141 atattataac aaaagagaac aatatgtact tatatttttc ctttattaaa aatgatatat
    10201 ctaatattaa aggaacaaag taaatcgaat taaaagtttt tgtataatta caaagtataa
    10261 aaattaatat aagttctaaa aagtaagaaa taagtataaa tttattctca tttgataaag
    10321 aaaaaaatct aacttgatta tttattataa ttagaaataa tattatcata gttatgaccg
    10381 gatacaatgt attttcaaca tttttcaaat agcttacaac taatataaat aaagataaat
    10441 atcgtataaa agcaactaat ttttctctgt tcattaaagg cactcctcca aaaaaaataa
    10501 acttttccta tgaaaactag aataatatca taaaagaata aagtcaatag aagtatttat
    10561 taatattgat ggaaataaaa tcaatccaaa aacttgaata gtaaaattgt atactgacca
    10621 taatttaaaa gtgaaaattt aatgtattgg agtgaataat gaataataaa gaaaaaggtg
    10681 attttggaga aaaagtagca gttaattatt tgctatcaaa aggagcaaag atactagaaa
    10741 aaaattacag attaaagata ggggaaatag atataatagc aaaaatggaa gatgaaatta
    10801 tatttgtgga ggtgaaatca aggtctaaca taaagtttgg ctacccttgt gaatctgtaa
    10861 gcttcaaaaa aagaaaaaag ataattggag ttgcaagtta ttatattata aaaaataact
    10921 taaataacac ttccataaga tttgatgtta tagaggtata tttattagaa aaaagaataa
    10981 atcacataat gaatgctttt taaaaaagat aatgtttttg gaaaggatta aatatgttaa
    11041 gtattattaa ttcaagcaac ttagtaggaa tcgacagctt tttagttaag gttgaagtag
    11101 atgtaagcaa tggaatacct agttttaata ttgttggtct tccaggcaaa gaaattaaag
    11161 aagcaagaga aagagtaaag tcagcaattc ttaatagtgg atataaattt ccaagcacga
    11221 gaatagttgt aaatttatca ccagcagata taaaaaaaga aggagctttt ttagatttat
    11281 ctataagtat aggcctttta agagagttaa taaaaaaaga tgaaaattac ataagagaaa
    11341 gtatgtttat aggagaatta tcattagatg gaaaaataag aaaagtaaga ggtatattgc
    11401 ccataattat gggtgcaaaa actcagaaca taaaacgtat ttttatacca attgagaaca
    11461 taaaagaaag tctatttgta gatgaaattg atataattcc tattaaaagt ttaaaagaat
    11521 gtgtcgattt tttaaacgaa gagataaaag tcgataaagt aaggataatg tcttttttgg
    11581 atgataagtc aagaaaagag aacgaagaat tagaaaaaga taatagttat atagattgca
    11641 aatatactaa gattaataat gaagaaagta aatatgatga agactttaaa gatgtaaaag
    11701 gaaattattt tgtaaagagg agtgcagaaa ttgctgctgc tggaaatcat aatatgttta
    11761 tgattggacc tccaggctca ggaaaaacta tgattgcaaa aagagtaagg acaattttac
    11821 cagatataag tgtagaagaa atgatagaag taagtaaggt ttatagtata ttgggtatga
    11881 taaatgaaac taaaggaata attgataaaa gaccttttag agcacctcat catacaacta
    11941 caaagcaatc tttaattgga ggaggaatgg atgcaagacc tggagaaatt gctttagcac
    12001 atagaggtat cttattttta gatgagatag ccgaatttga tagaaaaata cttgagactt
    12061 taagacaacc tatagaagat ggatatgtaa atatatccag agtaaagtac agtgcaaaat
    12121 atccttgtag agtactgtta gttgcagcta tgaatccatg tccatgtggt tattatatgt
    12181 cagagacaga gtgtagatgt agaagtaacg aaattgatag atacataaat aaaatatcag
    12241 gtccattgtt agataggttt gatatttttg tagaagttaa ttctataaaa tattcagatt
    12301 ttaattcttt aaaacaagaa gaaagctctc aaaaaataaa gagaagagta gaaaatgcta
    12361 gaaaaataca gataaataga tttaagaagg ataatataaa aaataatagt gaaattaaag
    12421 cttacaattt atttaaatat tgtaaactgg aaaaagaagc ttcaaaaacg gctgagatga
    12481 tatttaataa atacaaccta agcagtagaa gttatacaaa acttttaaaa atggcaagaa
    12541 caatagcaga tttagaagaa agagacttga taaattcaca gtgtattata gaagcttttt
    12601 catttagaaa ggcttattat agttatttta aatagttttt tgaggtggat tttatggaaa
    12661 aaagagatac ttatttatgg ttaaaatcaa ttggtggaat aacaacaaaa acaattgaaa
    12721 taatagaaaa tgaaatagta aatattgaag atatatttga tttttctgaa aaagaaatat
    12781 ataatttaaa aaatataagc ttgaatatta gaaaaaatat agtaaaatat agaggtcacg
    12841 cttatttaga aaatataaaa gaactacttt acaaaaaagc gattaaatat atttgtaaat
    12901 atgataaaga atatcctgaa aatttaaaga atatatacaa cgctccaaag ttattattct
    12961 acaaaggtga cataggctta gttaataata attttaatat agctatagta ggttcaagaa
    13021 aacctacagc ctatggaatt aattgtgcaa aaacaataag ttgtcaacta tcccaatatg
    13081 gagttaatat agtaagtgga ttagctatag gaatagatgc atactcgcac ataggttgta
    13141 tgagtggaaa atctaaaact atagcagtac ttggttcagg agtagacaat cctcttccaa
    13201 aacaaaattt acatttatca aataaaatat tagaaaatgg gggattatta ttatcagagt
    13261 ataatattaa ctcgacagta gccccgtacc atttttctaa tagaaataga attatcagtg
    13321 gtttaagtga tggagtagtt gttgtagaag cagcaataaa aagtggtgca ctaatcacag
    13381 tagattttgc tcttgaacat ggaaaaaatg tttttgcaat acctggtaat ataaattctc
    13441 aaatgagtag aggttgtcat aaaattataa aagaaggtgc aaaattaatt gaaaatatag
    13501 atgatatttt aaatgaatat aacatattta atattattga taaaaaaata aatcaaaaat
    13561 atgataatat aagtctaaat gccaagtcta agcaaataat tgaagctata aaaagagaag
    13621 gaaacttgca tatagatagt atttgtgatt atactggtat agaaataaag tatgtaaatt
    13681 caataatcaa tgaattagta ctaaatgagt tagtagtaga aatgaataat aaaacgtaca
    13741 gtttaaatgt ataaattaag attaggtagt gggggtgtat ggttttatgg ccaaaacatt
    13801 agtcatcgta gaatcgcctg caaaagctaa aactatagaa aaatttttag ggaaaagtca
    13861 ttacacagtt aaagcatcag ttggtcatgt cagagattta cctaaaagta aattaggtgt
    13921 agatatagaa aataattttg aacctcaata tataaatata agaggaaaag gcgatgtaat
    13981 aaaagaatta aaaaaagaag ctaaaaaatc taaacaagta tatctagcaa ctgaccctga
    14041 tagagaaggt gaagctatat catggcattt agctcatata ctaaatttag atgaatctga
    14101 ggattgtaga atagagttta atgagataac taaggatgca attaaaaaag caattaaaca
    14161 tcctagaaat attgatataa atttggttga tgcacaacag gcaagaagag tgctagacag
    14221 acttttaggt tatcaaataa gtcctatatt atggcaaaag gttagaaaag gtcttagtgc
    14281 aggtagagtg caatctgtta caacaaagtt aatttgtgac agagaaaaag aaataaaggc
    14341 atttattcca aaagaatatt ggacaataga tgttgaggct aagacagtaa aagacaaaga
    14401 tataactttg aaattttatg gtaaaaatga tgaaaaaatt gaattagaaa atgaagaagc
    14461 tgtaaatgaa atactaaaag aaatggaagg taaagattta aaggttgaga agatagaatc
    14521 taaatcaaga cgtaaatcag ctccaaaacc atttacaaca agtatgcttc aacaagaagg
    14581 agcaaataag ttatttttta ctactaaaaa aacaatgatg atagcacaag aattatatga
    14641 aggtatcgac atagaaggtg aaggtactgt tggtcttata tcatatataa gaactgactc
    14701 taaaagaata tctgatgaag caaaagaaaa agctaagggc tatatcgtaa acaatttagg
    14761 agaaaactac tataaaaaca ctggtgataa aaaagaaaaa aaggatacta aaaaggttca
    14821 agatgcccat gaggcaataa gaccaacctc agttgataga actcctgaga gtataaaagc
    14881 ctcattgagc aaagaccagt acaagttata caatttaatt tggagaaggt ttgtagctag
    14941 tcaaatggaa gattcagtgt ttgatatact aaatgtagaa tgtaaaatag gcgatattgt
    15001 atttaaggca actggttcaa aaatgaaatt tgatggttat accaaggtat ataatttttc
    15061 agaaagagaa gacaaaattt taccaagtat agaaaaagga gatatattaa atatagaaaa
    15121 tatattgcca gaccaacatt ttacacaacc accagcaaga tatacagaag ctagtttagt
    15181 aaaaacacta gaagaactag gtataggtag accaagtacg tatgctccaa caatagctac
    15241 tatactgaat agagagtatg ttgaaaagca aggcacaagt ctatgtccaa cagaacttgg
    15301 aataatagtt actgatattt tggaaaataa tttccataaa tttatagatg tagattttac
    15361 tgctgatatg gaaagtaaac ttgatgttat agaagaaggc aatacagatt ggaaaaagat
    15421 tgtatcagaa tcatatgcac cacttaaaga ggctattgaa gaagctcttg aaaatataga
    15481 aaaagtaaat atggatgaag aaactgatga gatttgcgag aactgtggtt ctaatatggt
    15541 tatcaaatat ggtagatttg gaaagtttat ggcttgtaaa aactatcccg attgtaaaac
    15601 aactagacca attatgaata aaattggtgt taaatgtcca aaatgtgaag aaggagatat
    15661 tatacttaga aaatctaaaa gaggtaaagc tttttatggg tgttctaatt atccaaactg
    15721 tgactttgta gcttggaata agccaacagg agagttttgt gagaagtgtg gttcatatat
    15781 ggttgaaaaa gtgactaaat ctgaaacaaa aacagtgtgt tctaataaag aatgtaataa
    15841 agagacaaaa gagcataatg acggaaaaga gtgaaaattg aaaaaaatca cataattctc
    15901 ttgaaaaagg ttccaatcta tgataatctt tcaagtgtaa acaactaata gaaaaataaa
    15961 ttttcattaa atgtgaaaat aatattagaa aatataaaag gttattaaaa ggcctaaatt
    16021 tcatgaggag atgattaaat ggcaagtgaa gtgttacaaa aaacaaggaa aataaataaa
    16081 acattacaaa caagtggtgg aagcagtgtc tcttttgatt tactggccgg agcattgggc
    16141 gacgttttaa gttctaatgt ttatgtagta agtgcaaaag gtaaagtact aggtcttcat
    16201 ttaaatgatg ttcaagacag ttcagttata gaagatgagt atactaagca aaagaaattt
    16261 tcagatgaat atactcaaaa tgtgttaaaa attgatgaaa cattagaaaa tttaaatggt
    16321 gagaagatat tagaaatctt tcctgaagaa catggaagat tacaaaaata tactacagta
    16381 gttccaatat taggaagcgg tcaaagatta ggaacattgg tactttcaag atattcaaat
    16441 tcattcaatg atgatgattt agtaatagct gaatacagtg caactgttgt tggtcttgaa
    16501 atattaagag caataggtga agaattagaa gaagaaatga gaaagaaagc tgtagttcaa
    16561 atggcaatag gcactctgtc ctactccgag cttgaagcag ttgaacatat ttttgctgaa
    16621 ttggatggaa aagaaggtct acttgtagca agtaagatag ctgatagagt tggtataact
    16681 aggtctgtaa tagtaaatgc acttagaaaa tttgagagtg caggtgtgat agaatcaaga
    16741 tcattaggta tgaaaggtac tcatataaga atacttaatg acaaacttac agatgaatta
    16801 aaaaaattaa aaaacaatca ataatctata ttttataggt ttagattaga aaaggtatct
    16861 atttcaaata gatacctttt ttagacgaat tattaaggta atgataagta tattaaaaag
    16921 gagtgagaaa atgcctttag cagataaaat aagaccaaaa acaatgaatg atgtaatagg
    16981 tcaaagtcat ataataggta atggaaaaat attgtctaag atattacaat ctaatttttt
    17041 accaaatatg atattttttg gtcctcctgg tgttggtaaa actacagtag cggaaataat
    17101 agcagaaaga agcaataaaa gcttctataa aataaatgct actaattcat ctttagaaga
    17161 tataaaaaag gtgatatcag agcttggaag tataaataat gtaaatggag tcctattata
    17221 tatagatgaa atacaaagtt ttaataaaaa acagcaacaa tcaatattag aatttatgga
    17281 aaatgggagt ataactttga ttgctagcac tacagaaaat ccatatcatt atgtatataa
    17341 agctctttta agtaggtcta ctgtatttga atttaaacct ttagaaaaac ttgatataga
    17401 aaaaggactg aaaagagctg ttgaagtatt aaatgaagat agctacatgg atattgagtg
    17461 caataataat gctattgaag atatagcaat tctttctgat ggagatatga gaagagcctt
    17521 aaatattttg gaagtggtag tctattcaac taaaccaaat aaggataata taacttatat
    17581 agattcagat gtaataaggg catctacatt taataaaatt ataaactatg acaaaaatgg
    17641 tgatagtcat tatgatattt taagtgcatt tcaaaaatct ataagaggaa gtgacccaca
    17701 ggcatctata cactatctag caagacttat aaaaggtgga gatttgataa gtatatgtag
    17761 gagactttta gtaatagcta gtgaagatat aggtttagct tatccaaatg ctattgtaat
    17821 tgtaaaggca tgtgtagact cagctatgca acttggattt ccagaagcga aaataccatt
    17881 agcagaagct actattcttt tggctacatc tccaaagtct aattctgcat gtatagcaat
    17941 aatgaaagct atggatgaac tggataaaga gtttgttaag gatataccaa atagtataaa
    18001 ggatgctcac tattctggtt ctaaagaaat aggtaggggt tgtggataca aatatcctca
    18061 taattttaaa aatcattatg taaagcaaca gtatttacca gatagcataa aagatagtat
    18121 ttattacata ccacaagaaa ataaaactga gaaaaatata aaaaaatatt tagaatatct
    18181 aaatagtgat ttttagactg aactttatgt atcgggggta gatattatga ttataaaact
    18241 taactcaact tatcacagta gagttatgaa atacttaaaa aaagaacctg agtacaattt
    18301 gtttataata ggggatattg aaagatatgg atatggaaat aattttttaa atatatgggc
    18361 agatgtgggt gaacatggtg aaataaaggc aatattactt aaatattttg aatttatgat
    18421 gttttattct gatggagagt atgatgttga aggtttttat aatctacttc gtaatactaa
    18481 ttacgaagaa atatctggga aaatatgtgc tgtagatgct ttagcaaaga gattaggact
    18541 taataattta aaggtagttg atttttgcaa acttcaaact aaaaagtttt tgatagataa
    18601 taattgtaat gcaaaggtta agagaattag gcttggaaat ctaaaaaaga cagtaaaact
    18661 ttatgactta atagatgaat ttcacagtac aactttagag aatttaaaaa atggactaag
    18721 gacaggtaga ggatattgta tagagattaa caaacaggtg gtgtcaatgg caaagagtac
    18781 ttcagaaaat aggactcatg caatgataat tggagtagga acacatccta aatatagagc
    18841 taaaggactt gctacaaaat gcctaattaa actatgttca gagttactga gagaaaacaa
    18901 aattccttgt ttgttttatg ataatgaaga ggctggaaag atatataaaa aactaggatt
    18961 tgaaaacatt ggaaaatggg gaatttattc aaaataaaaa attaaaatta atcttgacaa
    19021 aattagtagg gtataataaa attatcttga aagtaattcc tactaaaaca gtatgaatta
    19081 gatttctata tagaggtgat aatatatgaa gttgtctact aaaggtagat atggactaaa
    19141 agcaatgttt gaattagcat taaatcaaga caatggacca gtatcattaa aatttattgc
    19201 taagaagcaa aaaatatcag atcagtactt agaacaaata ttttcatctt taaaaaaatc
    19261 gggtctagta aaaagtgtga gaggtgctca aggaggttat ttgctttcaa agaatgctga
    19321 agacataaca gtaggagata tacttgtagt attagaagga ccagttgcat tatctgactg
    19381 tgtactagat gaagatgtat gtgaaaactc aaacatgtgt gtaacaaaaa tagtttggga
    19441 aaagatgaaa aagggtatag aagatgttat agattcaatt actcttaaag atatgataaa
    19501 tgactataac aaaaataaat tagaaaatga tataacaaat attaaaaaat agatttggag
    19561 atgaatgtaa tggaaaaaag aagattgtat atggattatt ctgcaacaac acctattaaa
    19621 aaagaagtat tggatgcaat gatgccatac ctaacagatt attttggtaa tgcatctagt
    19681 tttcacacat tcggaagaga agcaaaagat gctttggata aagcaagaga acaagtagca
    19741 gcccttataa atgctgaacc tagtgaaata tattttacag caggtggttc agaaagtgat
    19801 aactggacac tagagggtgt agcttatgct aataaaaaca aaggtaatca tataataact
    19861 tcaaaaatag agcaccatgc aatacttcat acatgtgaat acttagcaaa acatcatgga
    19921 tttgaaataa cttatttaga tgttgatagc gaaggaaaag tagacttaaa gcaactagaa
    19981 gattcaataa aagatactac tatacttata agtataatgt ttgcaaataa tgaaatagga
    20041 actatacagc ctattaaaga aatatccgaa atagctaaaa aacataagat attattccac
    20101 actgatgcag tacaagctac aggtaatatt ccagtagatg ttaaagaatt aggaatagat
    20161 ttgatgagta tgtcttcaca taaaatatat ggaccaaaag gtgtaggagc tttatacata
    20221 agaaaaggtg taagacttca taactttgtc catggtggag cacaagaaaa aagcaaaaga
    20281 gctggtacag aaaatatacc tgcaatagtt ggatatggaa aagcagcaga attagctaag
    20341 gaaaatatgc aaaatcatgt agaaacttta actcgtctta gaaataagtt aatagatggt
    20401 gtgttagaaa gaatacctta cacaagagta aatggtagct tagaaaatag attgccagga
    20461 aatgcaaact ttgcattcca atttatagaa ggtgaaggaa tacttttatt attagatatg
    20521 ttaggaattg ctggttcaag tggttctgca tgtacttctg gttcattaga cccttcacat
    20581 gtacttcttg caataggttt accacatgaa atagcacatg gttcattaag acttacagtt
    20641 ggagatttta caacagatga tgatatagat tatatactag agaacttacc aaaagtaata
    20701 gaaagactta gaagcatgtc accactttat gatgatgcga agaaacaagg tttagtaaaa
    20761 tagacataaa gataaagcat aaaaaataga cataaattga gtaggaggaa aagataatgc
    20821 aatatagtga taaagttatg gaacatttta tgaatccaag aaatatggga gaaattgata
    20881 atgcaagtgg tgtaggtgaa gttggaaacc ctacatgtgg agatataatg aagatatttt
    20941 tagacataga tggagatgta ataaaggatg ttaagtttaa aacatttggc tgtggttcag
    21001 ctatagcaag ttcttctatg gcaactgaaa tgataaaagg aaaaactata aaagatgcat
    21061 tagaacttac aaataaagct gttgcagaag ctttggatgg tttaccacca gtaaagatgc
    21121 attgttcagt tttagctgaa caagctgtaa aagcagctct tatagattat gctcaaaaga
    21181 acaatatcca tataccagaa ttggatggat atgttataga tgatgctcat gaccatgatg
    21241 ttgaagaaga agaataaaaa taagtgattg tgttatgaga agtttacaat gattaaaaag
    21301 aagtactaat gatataatta ggacataaaa aatagctgtt tttaaaatgg ctatttttta
    21361 ttgcctaaat taagtatttt aacttgattt agaggttaat tagttaacaa aataatccat
    21421 aagattcaaa taataaagat gattattttt ttagaaaata gaacaagcta tattttaaat
    21481 aagtacaata cttgctctat atggtataat aataagtaat gcaaaagatg aggtgaaatt
    21541 tgtttatgaa taaaaaagta atgataggta tgagtggagg agttgatagc tctgtagctg
    21601 catatctttt aaaacaacaa ggatatgatg tcattggtgt tactatgaag ttgtggcaag
    21661 atgatgatgt tgttgaaatt gaaggtggtt gttgttcttt atctgctgtt gaagatgcta
    21721 gaagggtagc aaataaaata ggtataccat tttatgtttt aaattttaga gaagtgttta
    21781 aagaaaaagt aatagattat tttatagatg aatatttgga aggaaaaact cctaatccct
    21841 gtatagcttg taataaacat ataaaatttg atgattttta taagaaggct agacaaatag
    21901 gttgtgatta tgtagctaca ggtcactatg caaagataga aaaggatgaa agtacaggaa
    21961 gatatttgct aaaaaaatct gttacagata aaaaagacca aacttatgca ctttataatc
    22021 ttacacaaga gcaactagag catactttac taccaatagg tgactatgaa aaagatagag
    22081 taagagaaat tgctaaggaa atgggtatgg cagtacacaa taagcctgat agtcaagaaa
    22141 tttgttttgt taaagataat gactatgcaa attatgtaaa aaaacattct aaaaagcgta
    22201 ttgaagaagg tttttttgta gatactaaag gcaatatact tggaaaacat aagggtatat
    22261 tatattatac tataggccaa agaaaagggc ttggaattac ttttggaaaa ccaatgtttg
    22321 taatagacat aaatccaata aataatacca tagttcttgg agacaatgaa gatttattta
    22381 aaaaggaact tattgctaaa gatgttaact ttatatcaat agatacgtta gaagaaccct
    22441 taagagttca agccaaaata agatattcag caaaaccatc accagctact attcatagag
    22501 taggagaaga tactattaaa atagtttttg atgaagctca aagagctatt actaaaggtc
    22561 aatctgtagt aatgtatgat ggagatattg tagtaggtgg aggtataatc gagaaaagtt
    22621 tataaaaaca tctaaaaaat taaaaaaata aacaataaag tatattaaga gtaaataaag
    22681 tttataaaat aaatttattg tatatgaaaa tatagtattg attaattttg taaaatttag
    22741 taatataatt agtatattaa ttaaaaaaca aatacagtga taaggaatag taaaatattt
    22801 gatgtcttac agagagaggg aaatgctgga agcccttaga atatgatatt tgaagcagcc
    22861 ttttagtagc aatcttgaac aatttatctt ttataaagaa gatttaagta gagattgacg
    22921 gtagttacgt tatagctaag gttttccgaa gatatcatac tttgtatgat aattagggtg
    22981 gtaccgcgat tgtcctcgcc cctataaatt tatttatagg gacgaggttt tttatttaaa
    23041 cgaaaaaata aaatttatta ggaggaacat gatatggaaa agatgggatt aaatgaaata
    23101 agaagtaagt ttttagaatt ttttgagtca aaaggtcatt atgtagcaaa tagttattct
    23161 ttagtaccaa acaatgataa gagtttgtta cttataaact caggtatggc acctttgaaa
    23221 aactactttt caggtgtaga agtacctcca agtgtgagaa tgtgtacatc tcaaaaatgt
    23281 ataagaacag gagatataga aaatgtaggt ataactgcaa gacatgctac attctttgaa
    23341 atgatgggta acttctcatt tggagattac tttaaaagag agtcaatcaa atggggatgg
    23401 gaatttgtta cagaatggct aaatatacca gaagataaga tatgggtgac tgtatatgaa
    23461 gaagatgatg attcttatga tatatgggca aaagaaatga atttcccaga agaaagaatg
    23521 gttagacttg gaaaagatga taatttctgg gaaataggta caggtccttg tggtccttgt
    23581 tcagaaatat attttgatag aggagaagaa tatggatgtg acaatccaga ctgtaaacca
    23641 ggctgtgatt gtgataggta tttagaattt tggaaccatg tatttactca atttgataga
    23701 gatgaagaag gaaattatag tctattagaa aacaagaata tagatacagg tatgggtctt
    23761 gaaagaatgg gatgtattat gcaaggcgta gacactatct ttgaagttga tacaattaaa
    23821 tctatactag aagcagttga gaagttaaca ggagtaaaat atggagagaa tccaaaaaat
    23881 gatatatcaa taagaataat aactgaccac ataagagctg taacattctt agttagtgat
    23941 ggagtgcttc catcaaatga aggaagagga tatgttctta gaagactcct tagacgtgct
    24001 gctcgtcatg gtaagttatt aggagtaaaa gaattatttt tacagaaact aatagatgaa
    24061 gttataaaag taaatgataa agcatatcca gttttagttg aaaaagaaag ttatattaaa
    24121 aaagtagttg gaatagaaga agaaaaattc aatgaaacta tagaccaagg aacagaaata
    24181 ttgaactctt atatagaagt attaaaaaat gaaggaaaga ctgtcctaag tggtcaagaa
    24241 gcttttaaac tatatgatac ttatggcttc cctatagact taactaaaga aatattagaa
    24301 gaagaacatc tatctgtaga tgaagaagct tttaatgaag aaatggaaaa gcaaaaagaa
    24361 agagctagaa atgctagagg aaatatggat ggagaaagtt ggaaggaaga ccctctatca
    24421 aaattagaat ctacagttga cagtacattt aatgggtata gtgaaattta tggtgaagga
    24481 actattgaag ctatagttaa agatgatgag ctagttcaaa gtgcagaaga aggcgataag
    24541 gtatctatag tactagataa cactactttc tatcctgaag gtggaggaca agttggagat
    24601 tgtggattga taactaatga aaacttagtt ttagaagtat taaatactaa aaaaggtgct
    24661 aataatagta taaaacatat aggtataata aaatcaggaa gaataagtaa tggggacaaa
    24721 gtaaagacac tcgtagatag agaaactaga atgtcagctg caagaaatca cagtgctaca
    24781 catttacttc ataaggcatt aagagaagta ttaggagaac atgttaatca agctggttct
    24841 ttagtaactc cagaaagact tagatttgat attactcact ttgaagcaat atcaaatgaa
    24901 gaattaaaag taatagaaga aaaagtaaat aatgttattt tatcatcttt agacataaaa
    24961 tgtgatatta tgaatataaa ggaagctaaa gaaaaaggag ctacagcact atttggtgaa
    25021 aaatatggtg atgaggtaag agtagtttct atgggagatt attctacaga actttgtgga
    25081 ggaactcact tgacaaatac ttctcaagta ggaatgttca aaatattatc tgaaggtgga
    25141 gtagcagcag gtgtaagaag aatagaagca ataacaggaa aagctgttta tgaataccta
    25201 aaagaaagag atggaattat ctctgaagtt tgtgtaaact taaaatctaa agaagataat
    25261 ttaattcaaa gaataagttc tttattagaa gaaaataaaa acttatctaa agaattgcat
    25321 gatatgaaag ctaagatgag tttacaatca gttgattcta tttttgattc taaagttgaa
    25381 gtaaatggag ttaatttgat aactaataaa tttgaaggta tggatatgga tacactaaga
    25441 gaaacagcag ataatctaag agataagctt ggttctggtg tagtagtact tgcaaatgta
    25501 gtagatgata aagttaactt tgtagttact gccactaaag atgtattaga taagggaata
    25561 cattcaggta atattgttag agaagttgca aaaatagctg gaggaaaagg tggaggaaga
    25621 cctaatatgg cacaagcagg agctagtgat gtatctaagg ttgaccaagc tttaagctat
    25681 gcaagtgagg ttattaagac acaagttaaa taacattcac tcaaggaggt ttttagtttg
    25741 gaaaaagatt tagactacac tatgaaattt gaaggaatac cagaagatag gatgagtgta
    25801 ggagatacta ttgattttgt atataaagca ttagttgaaa aaggatataa ccctataaat
    25861 caaattatag ggtatctcct ttctggtgat tctagctata taactagtca taaaaatgca
    25921 agagctataa ttaaaaaatt tgaaagagat gagatacttg aagaagtaat cacgcactat
    25981 ctgaatagaa agtaggtcaa ataaatgaga aaaaagatta tgtcgttgtt tgcaatagtc
    26041 attatgattt ttgtatcaac tgctagctat ggttttgctg aagaaaaagg caaggtcata
    26101 tttattgata tgaataggac caatcttgga agtatgatga atattccaat cttgaaaaat
    26161 gagatagaaa agagaggcta tgtagctctc atgaatataa gaggggatca aggtactgat
    26221 gatagaaggt cttatgcaag tatgggtgca ggaacaagag ctagtgtaac tactgaagac
    26281 tatatcaatt ttcaaaaagt agaccaagat actgctaaag cttttgaagt cgcaacaggt
    26341 caaaaagcta aagaaataaa tgatttaggc attaatagat ctataaatga aaatcttgag
    26401 tatggagaat atggagcaaa tttagggtca ttaggaaaaa ctttatcaga aaatggttta
    26461 aagacatctg taattggaaa tgctgatata attgaaaata atcaattagt taaaaacaga
    26521 aacttatgtt tagtagctat ggaccaatat ggaaggattg attcaggtaa tattgataat
    26581 attaataaga aagattctaa aatgccattt ggaatatcta cagattatga taaattacta
    26641 gatgaaacta aaaaatccta taaaaatagt gatgctatat ttgtagaact tggagataca
    26701 tatagattag atatgtatag attaaatcta aatgataata cttatgatac aatgaagaaa
    26761 aatattgtaa aaaatataaa taaatatctt gaacaagtat tttctatggt tggagaaaat
    26821 gacactgttt atatagcaag tgcatttcca agtgacctag attataagaa taagagaaga
    26881 ctttctccaa taataaaatt tagtggagaa gggaaaggtc tactggaatc tgccacaaca
    26941 agaaaggctg gaattgttgc caatttagat gttggagtag atatactagg tacatttggt
    27001 cttgaaaata aagagatggt aggtagaagt tatactttag taaataaaga ggacaatatt
    27061 agctatctaa aatcacttta tgaaaggata gtgtctatat cttcaataag gtcaactatt
    27121 ataaatggct ttgtaggcgt aatatctgct tcttgggtaa ttgccatgtt tgcgattttc
    27181 tttagaaatt atatgccaca taaagagcgt gtattcaaag tacttaagga gtttataaaa
    27241 ttaggcataa taatgccact atcatttatg ttagctcctt tgatgaattt taaaactcca
    27301 atttcaatat ctttgggaat atttgcgact acgctaatat tatacttgtt aggtagaaaa
    27361 ttatttaagg acaatgatat aaagcacatg ggattttttg caacacttac tattttagtc
    27421 atagttatag attctgtatt tggaacatac ttaatgcaaa ataacataat gagttatgat
    27481 gctgtcatag gagctaggta ctatggtgta ggaaatgaat atgaaggtgt aactatagca
    27541 agtgcagtat ttggcttagc agtattatta cattataaga aggtatctaa attacttgtt
    27601 gtaatttttt cattaataat acttataact agtgcatatc cttcaatggg tgcaaatgtt
    27661 ggtggagcta tatcagaatg tgtagcatat ttgttgttta taatgcttat atatgacatt
    27721 aaaatagatt ttaagaaagc tgtattatta gctgtttcag ctgtagttgt cgtttttgca
    27781 tttgcagctt tagatatact ttcaggtagt gaatctcatt taggtatgtt tgtacagaga
    27841 attttcctaa atggaccagg agaaatatta caaacatttg gaagaaaaat tcaaatgaat
    27901 atgcaactag cacagacaag tgtatgggta aatattttac ttgtaggaat tggagtaatt
    27961 gcagtgctta tatttaaacc atctagacac tttagaaaga taatgaataa ttttccaatc
    28021 ttgtttaaag ggtttattgc atctatggtt ggttgcataa ttacactttt agttaatgat
    28081 tctggaatag ttgctgcatc tacagcatct atatatatac ttataccact aataataata
    28141 agtataaata tgataatatt tgaaaaaaat aactaaaaaa atttttactg ttcatatagg
    28201 atttaaataa aaaatgcaat atgattatta atgtaaactg gatgcttaca taaaaaatca
    28261 tattgcaatt tttatgattg attatgtaaa attatatgaa gcagtaagcc tgtgacctta
    28321 gttatgagag gttcagaata tgtatttaat ctatagaata taaatagata aaggaggaac
    28381 tatgttagat ggaagaataa tgggtcttga tgttggtgat aaaactattg gagttgctgt
    28441 gagtgatttg atgggattaa ctgcacaagg tgtaaaaaca ataaaaagag ttggcaagaa
    28501 aaaagatatt gaagagctaa aagcaataat aaaagaaaaa caagtaaata aaattgtatc
    28561 aggattacca aaaaatatga atggaacttt aggtccacaa ggagaaaaag taatcaagtt
    28621 ttgtgaattg gtaaaagcag aaacaggtat tgatgtagag ttttgggatg agaggctttc
    28681 tacagtagcg gcagagaggt ctttattaga agcagatgtt agtagacaaa aaagaaaaaa
    28741 ggttatagat atgttggcag cagtaataat tttgcaagga tatttagatt ttaaaataaa
    28801 ttcataaaag aactataaaa ttgaatactt atgatatata atataaataa taaaaataaa
    28861 aataaatttg aggtgaatga cattatggaa gaaaatataa taaatttaat agacgaaaat
    28921 ggcgttgaaa gccaatttga aataatatta acattagaag cagaaggaaa agagtatgca
    28981 atattaatgc ctttagatga tgaagaagct gaagaagctt taatctttag aattgatgag
    29041 gatgaagaag gtgaaatctt aatacctttg gaaagtgatg aagagtatga aactgttgta
    29101 gctgtgtata ctgctataat ggaagaagaa ggtttaaatt acgacgaaga tgaatcaaat
    29161 ggacttaact agataagtaa ttaagtttgt atataaaaat cagatattta ttatatttgc
    29221 tttaattaaa ccttattaat tgtaaaagca tttatagtat atatctgttt ttttgttttt
    29281 tattaaaaat accaaaaaat ttatgaattt tggtgaataa tatatctttt atgggtatat
    29341 atttatatta tgagaataaa ttaaatttat ttaaatgtct tgacaaaaaa tgaattatca
    29401 attacaatat tatttaataa agattatcaa ttgaaaatag taagggtgat gttatggcaa
    29461 atacaatgga tttattaaaa gacaagttaa aagaaactgg ttttaagatt acgccacaaa
    29521 gaagagcaat agttgaaata ttgcttaaac atgaccattc acatctaagt agtgaggaaa
    29581 tctatgattt agttagggtt gactgtcctg aaataggctt agctactgta tatagaacaa
    29641 tgcagctatt ggatgaaata ggattaatat caaaactaaa cttagacgat ggatgtataa
    29701 gatatgagat tagtctacat aaagaagact gtcataatca tcatcatctt atttgtaaaa
    29761 attgtggaaa gataatggaa gctaaagaag accttttaga caatatagaa aaagagatac
    29821 aatcattgta caaatttaaa atacttgatc atgatgttaa attttatgga ctatgtgacg
    29881 agtgtaatgg agtaagcgat tcagaagaat aaacttctat tgcttaatgt cgatggaaga
    29941 atagtataga acttttatat tctatgctat tcttccattt tttattataa accatctata
    30001 aaatgcaaat atacagtttt caagatactt atagttttaa tattgaaaat ataattaatc
    30061 ctataagtac aaaaatgaat gatttttcta tgaaattact aaatggtcct ccaacagtta
    30121 aaaaacctaa agaaattttc ttttcgctta gcggataaaa aattggtatt ccttgctttg
    30181 taaataaatc tcctaaaaac atatgacttg cataaccaat cgtaccataa aaagataaat
    30241 tattaatatt agtgattaat tctatctgtt ttagtaaaaa atatactatt atagttgcaa
    30301 atatgctatg agaaaaactt ctgtgcttta accagggaaa aattgacaac attagacaaa
    30361 aaattgtaaa atatatttca acttctatga tgtataagca tatagctaat agtataaata
    30421 ttaatgatag aaaaactttt cttagaaatg tgtgggttag ttttgcttca ataattataa
    30481 ttgcaataaa tgtaattatt gcgcttaaat aaaaattatc atttatattt attgaaataa
    30541 agaaaataat aatattaaca gcatatataa aaattctata tatgtatttg gatacattgt
    30601 tttttagaat gaaatttgag accatagaat tagatttatc caaatcgggt agtacagcaa
    30661 aagtagctga aactaaaata tcaactaaag atataggaat tttaaatatt aaagatgttt
    30721 ggatagctgt taagattcct attgcacaat gagttttacc acgcataaat cctcctaaat
    30781 tatccgatat taaatcatat aatatttaga ggatatcaaa ataaaatagg tttgtcaaaa
    30841 taatacgtta aatgtatttt aaatatagct taaaatatga tacgaattat ggtacaataa
    30901 gaataactgg tagcgagtgc gtgatttgat attatttatg attgtagaac gtgttaaaaa
    30961 attaaataga cagaaggaga tgatgcaatt gttcaaaaag aacaccaaca agataaaggt
    31021 aatggccttg ggtggactta acgaagttgg aaaaaatatg actgttgttg agtataaaga
    31081 tgaaataatt gttatagatg ctggacttag ttttccagag gatgaaatgt taggagtaga
    31141 tatagttata ccagatataa cttatttagt aaaaaatagg gataaaataa agggtatatt
    31201 tattacacat ggacatgaag accatatagg agctcttcca tatatattaa agaaaataaa
    31261 tgtacctgta tatggggcaa gacttagtat aggtcttata caagtaaaac ttaaagaaca
    31321 taaaatgaat aatgttaagt taaatgttat aggtcctaga caagttataa agcttgataa
    31381 tatggaagta gagtttttga aaaataacca tagtatacca gatgcttact ctattgcaat
    31441 acacacagac caaggtataa tataccacac aggagatttt aagattgatt tgactcctat
    31501 tgatggagat gttatggata tgcatagaat ctgtgagttg agcaaaaaag gagttctttt
    31561 aatgctggca gatagtacta atgcagaaaa accaggattt actatgtctg aaaagactgt
    31621 tggggtaggt cttgacgaat tatttgcaaa aggaaatggc agaagaatta tagttgctac
    31681 ctttgcatcc aacatacaca gattacaaca aataataaat actgctgaaa agtttaatag
    31741 aaaagttgct atatcaggtc gctcaatggt caatgtagtt ggagttgcaa aagaattagg
    31801 atacttagat atttctgatg acatgcttat agatttaaat gatatatgta agtatgaaga
    31861 tagtgaatta gtgataataa caactggttc acaaggtgaa cctatgtcag ctcttgctag
    31921 aatggctttt tcagagcaca aaaaagtaga aataaagagt ggcgatttag ttataatatc
    31981 tgctcatcca ataccaggaa atgaaaaatt aatatcaaga gtaatcaatt tcttatttga
    32041 aaaaggagca gaagtagtat acagtgatat agctgatata cacgtttctg gacatgcctg
    32101 tcaagaagaa ttaaaactta tacatgcatt agttagacca aaatttttta tgccagctca
    32161 tggtgaatat agaatgttaa aaagacatgc tgaaatagct gaacagcttg gtatggataa
    32221 agaaaatatt tttgtcatgc aaacaggaga tgttttagaa ttagataaaa actctgctaa
    32281 ggtagcaaac cgtatacaaa caggaaacat attagttgat ggtctaggag taggcgatgt
    32341 aggtaatata gttttaaggg atagaaagca tttatcagaa gatggtctta tgatagttgt
    32401 agttactatt tccaaggatg agggtaaggt cttagcaggt ccagatataa tttccagagg
    32461 attcgtatat gtaagagagt cagaagattt aatggatgga gcaaaggata taattaagaa
    32521 tgttctaaat gaatgtgaag agaaaaatat taaagaatgg gcttatttaa agaataatat
    32581 taaagaaaac ctaaaagaat atttatatca aaaaactaaa cgaaatccaa tgatacttcc
    32641 tatcataatg gaagtataat ataaaaaaac tataaatttg aaaattagac ctcttgtaat
    32701 ttttaaagta atatcaaaaa tccaattgat attatcttaa gaattacaag aggtcttttt
    32761 aatactcatt ttaaaaaaat atatattaca taaaatacca attataacaa tcttttatta
    32821 gtaaaaacta cttatatata aattttactg ataggaggat tcttatggat gatgtttcaa
    32881 gacaaaatgc tataaaagct ttaaaacaaa caaaaatgga aatagcagga gagtatggaa
    32941 tgaattatga agatgctttt gaaataatag aaaatgcgtc taataaaggt gttttagaag
    33001 gttactttaa aaagcttgaa aagaaaaaaa atttaggtca aggtatatct aggcatttag
    33061 aataaaaaaa gttaaatgtg agaaaataag tttaaataaa ctttaagagg tgacatcgat
    33121 gaaaaaatta gctagtactg ctttggctat attaattgcg cttactcctt taagcttttc
    33181 ttttgcaaat aataaggaaa atgctgatgc caaccaacta aatatatctt caaaatcagc
    33241 aattttgatg gatgttggta gtggacaaat tttatatgaa aaggatgctc ataaaaagct
    33301 tcctcctgct agtgtaacta aggtaatgac tatgctactt atagtagaag ctttggattc
    33361 aggcaagatt aagcttgatg atgaagttca agtaagtgaa actgcatcta gtatgggtgg
    33421 tagtcaaata ttcctagagc caggagaaac tcaaaaggtt gatacattat taaaaggtat
    33481 agctgttgct tctgcaaatg atgcttgtgt tgctatggct gaacacttag ctggaagtgt
    33541 tgaaggattt gttgatagaa tgaatgctaa agcaaaagag ttaaatatga atgatactca
    33601 ttttgcaaat acaaatggac ttccagttgc aaaccactat acatctgctc atgatatagc
    33661 tttaatgtct agagaactat taaaacacga aattataagt aaatacttaa ctacatggat
    33721 ggataaggtt gtagtaggga agaaacaagt tacagtaggt cttgcaaata ctaataaact
    33781 tataaagcat tatcaaggag caactggtgt taaaactggt tttactcaag aagcaaaata
    33841 ctgtttatca gcatctgcaa agagaggtaa tactcatcta gttgctgcaa ctttaggagc
    33901 agagacttct ccagaaagat ttaatgatgc ctctagcctt ttaaactatg gatttgctaa
    33961 ttatgaaagt gttaagttat gtagtaaagg tgataatata gcaacactta cattggataa
    34021 ggctgatgaa aacaaagtta aattggttgc taaagaagat ttaaatgctc ttattaaaaa
    34081 aggaagttct aaagaatttg agaagaaaat tgaaatagta aaaaatccta agatgccaat
    34141 taaaaaggga actgttttag gaaaaataaa gatatgtaaa gataaaaaag taataggcga
    34201 agtggaattg ataaatacta aggatataaa taaggcaagc tatctacaaa tgcttcaaag
    34261 aataattgat aatatgatat aaataattat cagatgaaat ataattataa tacacaaaga
    34321 tatgattata atacacataa aaaataccct tatgtaatta tttgccatag gggtattttt
    34381 atttaccata tagaagtatt ttataatctt atgattttta tagaaatata gaaaagttta
    34441 tgtatcttaa aatatcattt gatttaatat catatatagg gttgtttaac aattatctaa
    34501 gtaaaggcat actaccagta agtttattca ctttcttttt attcccataa attgctatcc
    34561 caaaatactg taactcatct tctggaacac tagcaatctt ttgtgtaaat tcttcataaa
    34621 gattgcaact ttgtgctaca tctgaaaaat ctacaacagt taaatcgtta aattccaaag
    34681 tatataattt tttacgtaaa tcttttatta tttctttgtt tccttttaaa ataggtacag
    34741 gaatgtcaat aattcctaag tggctattac ctgttttgtc aattacatct gggccaacag
    34801 tttctggggc atgttttcct agtgtaattc ccattatagc tgctgtattg gatataattc
    34861 ccattggtaa attttcgtct attaccatga cacattttga atttttagtt tccattttta
    34921 tttctccttt ttaccttatt taaagttgtt tgaatcatca tttataaata tattttgata
    34981 ctgctttggt gtaagaccaa taaaattctt aaaaaagttt gtaaagtgac tttggtctac
    35041 aaatcctgtt tgtatcgcag catctatagg ttctacgcct ttttctaaaa gctttttagc
    35101 tttgtttacc cgtatatttt ctaggtatct atatggtgta atacctttta atttcgtaaa
    35161 attacgaagt agagagtact tattcatacc tgctatagtg cttaattcgt caagtactat
    35221 atgttcagca taattatttt ctagataatc acatactgct tgaatttcta tatttgtatt
    35281 ttccaagtta gattgcaaat ttggctctgt atgttcttca attaattgtt caattaggaa
    35341 gaaaaaaagc tcttcctttt taaaatccaa ttcttcttcc atgattatat agtgtaattc
    35401 ttgtagtaga ggtactagct cactgccaaa tataacaggt tgattaaatt ttggtaaata
    35461 atttttacct gtaatttcaa agacggtttt tttcattatt tctggcttaa tattaataca
    35521 tctgtaatca agtactttat catctatttg ttcacaagta tgactatcaa aagggttaaa
    35581 aagtagcaaa tctccagtac ttgtggtata ctctttattt ttacaagata aatatctttg
    35641 acctttttct ataaaaccaa ttacataatg ttcatgaaaa tggtttggga atttttgcat
    35701 tatgccttta aatctatatg cttcaatttt taattcccta tcaaagcata ctgtgcgttc
    35761 ttcttgtctc acttaagtaa cctcctttca tgttatacaa agtgtaacac atattttttt
    35821 atacttcttg tataatattg actaagagac aataatgtca attcatgtca atataatatt
    35881 atacttacag taggtgtaat tattatattt tcacaatgaa tatgatataa taataaaagt
    35941 tataaaataa aaagcaaatt ttaattttat gcctagaagt taaaattatt ataatgtaaa
    36001 ataattttga ttataagcta tttaattgtt ataataatat taaaaattta atttaggaga
    36061 tgtacattaa tgttaagtat gaatatagga gaaatagtag caagtttagt tggtatagct
    36121 atggcaatat caatacatga gtttggccat gcttattcag ctcacttgtt gggagatgat
    36181 acagctaaag cttatggtag aatgacatta aatcctgtaa gacatgttga cccaataggt
    36241 cttttggcta tgtttatcct taagataggg tgggcaaagc cagtaccagt aaatccaaat
    36301 aactttaaaa actataagat aggaaacctg atagtttcac ttgcaggcgt tttttgtaat
    36361 gttttgactg ctatactgtg tgtaattatc aataagtatg tgcatatgta tgctataaat
    36421 acaatagctg gatatgtgct tttatatagt atgggatttg cagcttttaa cttacttccg
    36481 atacctccac ttgatggatg gggagttata tctacatttg taccatataa atggaatgaa
    36541 tatttgtata aatttgaaag tatgagttat ataattttat taattgcttt atttactggt
    36601 atatatcaaa taatactatc accaatatat acagtgttgc taaaaatagt atatttattt
    36661 gtttaatata ataaacagat taaagaggga ttgatatgaa gtataatata caattacaag
    36721 tctatgaggg accattagat ttactttatg atttaataac aaagcataaa atagacataa
    36781 aggatatatc tataatagat ataactaagc agtatctaaa ttatttgaaa atgctagata
    36841 aaatggattt agagataact agtgaattta taactatggc atcaaaactt ttagagataa
    36901 agtctaaata tctgctttat aagcaaaaag atgaagaaga agacccaaga atagaactta
    36961 tggaaaagtt agaagaatat agaaagttta aggtagcatc acaagatata aaagaaaata
    37021 taacttatgt taatgagaga ttttatagaa ataaagaaga gataattatt gatgacaatg
    37081 tggatttaga agatatatca atagaagcaa taaaaaatat acttccatac atatttaaag
    37141 ttaaaacatc acagatagaa aatgctaatg atgagaaact agataaaata gtaaggaaga
    37201 aaataatatc tgtagaagaa aaaatattat atataagaga tattataaaa gataagattg
    37261 aagttacttt tacaaatatt ataaaatcat atgaaaatga tgaaataata gctacttttt
    37321 tatctatatt agagcttata aaagaaaaag aaatagtcgt agtacaagat atattttttg
    37381 atgatattct aataagaaaa agttcggagt gttaaaatga aaagagaaga tataaagtat
    37441 attatagaat ctgttatgtt tgcatatggt gagccaataa gtataaaaga attaaactat
    37501 ataataaata aagaactttc atcaaaagaa atagagatta tgttaaattt acttattgag
    37561 gagtacagag aacaaaatag aggtattcaa ataatcaagt tagagaataa gtaccaaatg
    37621 tgtacaaata aagactatgc agaatatata aagaagatta ttgaaccaaa gaaaaagaaa
    37681 agtttgagcc aagctacatt agaaactctt actataattg cctacaaaca accaataaca
    37741 aaggtagaaa tagaggatat aagaggagta aaatgtgata aggtattaca aactttgttt
    37801 gaaaatgaac ttataagaga agctggtaga ttgaataaga tagggaaacc aataatttat
    37861 aaaacaacag atgaattttt aaagttgttg aatatagaaa gtcttgaaga attgccacct
    37921 atagaaaatt atcaagaagt ggcaacaaat gaataaaaat ataaaatcac aataatttag
    37981 tatgtaattt taaattttaa aagatttaaa atgctctaag ttgttgtgtt ttttgtataa
    38041 atataaaatt ataattctct taaaataaat atggataatt aagtataaat agtaattttt
    38101 tgatgataat ataaacatgg agcttaatga attatttaat tttaagtatt taataatagc
    38161 tgtaattttt attataagtg taactatgtt gattttaata tctcatataa acatcttaat
    38221 aatagctgat ataaaaaaca aagatatttg tttaaagctt aatataaaat acatgtttaa
    38281 tttaataaat ataaatagac aactttatcc agctgaaaat agtaaaaata atgataaaaa
    38341 agaggggatg aagaataata tagattcaag tatattatta gctgatgacc ttttgagcat
    38401 ttatagatta ttaaaaaaga taaaaataca tgaactttat tcaaatatta attttggaac
    38461 tggaaatatt ggcttaacat cttctgtata tgtattgata aatactttgt atggaaattt
    38521 atttaatatg attgatgcag aaaaaatgta tttaaatgta aatccagatt ttacaaaaga
    38581 ttatgtttta ggaaatataa ggatacatat aaggccaaga ataaaagctt tatttaatat
    38641 tataataatg attaataaaa ttatgaataa aaataaagga aataaggaag gtgatagtaa
    38701 tgagagcaac aggttcgata cagagtctta tggaaacaac tcttgaaaca attaaaggtt
    38761 ctatagatgc aaatactatt ataggagacc ctataaaaac agatactaca gtggttgtac
    38821 ctatatcaaa ggttacaatt ggctttggta taggaggagg agaatactca aaaggatatg
    38881 atgataaaga tagggaaatt gaattaaaaa atgaaaaatc agatacgaat tttgctggag
    38941 gtagcgcagg ggctatatct gtacaaccag tagcatttgt agtagttgaa agtggagaga
    39001 ctagaataat gagtttagat agtaatatca atctagtgga taatatatta tctataactc
    39061 ctagagtatt ggagaaaatt caaaatatat cacaaagtaa taagaatcaa gataaaaaca
    39121 atatgtaaga ataaaatatt tttaaatatg tatatagttt taagtaaagt agtggggtta
    39181 atataaatcc cactattttg ttgtgtactt taaacattta tatctaataa gattttagtt
    39241 taaaacaatt taaatagata ataaagttga tatatagtat aattataaaa agaaaagaag
    39301 tattatatta tagatatata aacgcatttg agatataaga gggagttata aatcattagt
    39361 tttgaggtga atgttatgag cgataaagaa ttatctattt tgttgttaga aagtatgaat
    39421 aattttcata atttaataaa gattattaat aacgaaagat ataaaaaaga taaggttctt
    39481 acagaaagac agttctttgc cttagtaaaa ataagaaagc atgataaaat agagcttaaa
    39541 aatctaagta gagatttgca tgtatctact tctagtctgt gtatactact taataaattg
    39601 gttgagcaag agtatgtata tagggaagaa gatagtagag atagacgaaa tactttttat
    39661 ggaataacta aaaatggaga aaagatattg gataatgaaa tactaaagtt tgtatctata
    39721 ataagtgata aaatggattg cctagatata gataataaag ataaattatt tacttctttg
    39781 gaagaatcta aaaatataat agaacaatta ttttaaaaag gagaatttat tatgaatata
    39841 aagtattcta aagagatatt aaaaagttta gaaaatgcta taacagtagt agctctattt
    39901 gagggagaag atgtttctac gacatttgat aaaataaata tgataagcag aaattttaat
    39961 tctaaagaaa atagtataga tactgtaaca ttaattgaaa ataacaattt ggtaaatgtt
    40021 tgtcttgttg gttttggtaa gagagagtta ttgaatagag aaaaaattag aatcataggt
    40081 gggaatttgt gtaaaaaatt aaaaaatata ttgaaaaatg aagcatataa gagtagtgat
    40141 gttaatgtac tgaatctcaa tttaaatagt gatgaaatag gttgttttgt agaagggata
    40201 ttacttggaa attataagtt tgataagtat aagacaaaaa gtaaggattt tgaaagaaaa
    40261 gaagttgata ctataactat atttactgaa aaagaagttg agtgtcaaat agaaaaggct
    40321 aaaatattag ctaattcaac tataatagct agaaatctag ttaatgaacc atcaaacgta
    40381 atatatccaa aaacattagc agtagaaaca gttaaacttg gtgcagagtt tgggtttaat
    40441 gttgatgttt atgaagaaga aaaaataaag gcattgggaa tggatgcttt tttcgctgtt
    40501 tcaagaggtt caattaataa accaagattc atagtaatga gatactttgg agataaagaa
    40561 agtacagata tattaggtct agtaggtaaa ggattgactt atgatactgg tggatattct
    40621 ttaaaatcaa atgcttctat gctagatatg aagacagata tggctggtgc tgcgagtgtt
    40681 ataggtgcta tgtgtgctat atctcaaagt aaattaaaga aaaatgttat agcagtagtg
    40741 gctgcatgtg agaatgcttt atcaggaggt tcatacaaac ctggagatat aattagttct
    40801 atggcaaaga aaactattga agttttaaat acggatgcag aaggtagact tacattagct
    40861 gatgcaatat actacattat aaacaatgaa aaagtaacaa aggtggttga tgtagctaca
    40921 ttaactggtg ctgctttaac attgttaggt aatgttgcaa ctcctatagt aacaaataat
    40981 gatgattttt attgtgaatt agaaaaagct gccactttat caggggaaag ggtatggaag
    41041 atgccaattt atgatgaatt taaagatatg ataaaaggag aagaagcaga ccttaaaaac
    41101 actggtggta aaaatgctgg atgtataaca gcaggagctt ttattggtga atttgttggg
    41161 aatactcctt ggatacatat ggatatagcg gggacaagta catcatcaaa atcaattgga
    41221 tacaaagcaa agggggctac aggagaacct gttagaacat tgtactattt ggcagaaatc
    41281 taaaaaacaa ttataaaata taatttataa agccacgtgg atatttttgt aatttaagat
    41341 tatctatgtg gctttatttt ttatttaagt aaattattta ctctttttct ttttttcaag
    41401 tataaaagta tctctattaa ataaaagagc aggtatgtac ataatactag taacaaaaat
    41461 aaacgtaaaa atagcagttg tgctatgact taaaaaacca taatagtacc tagagttggt
    41521 aaatgtgttt agtaaaaata tgaatgaaaa ataaataaaa catatagcta agttatctct
    41581 aagtgcatat ctgttaactc taaattttct aacatcaacc atatatatat ctgcataaat
    41641 tatcaagcac ttagataaga taaagataaa atatattaag ctaggaaatt catagcccaa
    41701 gtttttagaa aaaatgagtt ctccaactga acaaactaaa ctccaagaaa aaaatatatt
    41761 aaaaagatga tactgcttaa aaattagtat ggctatacac agatatatac aaatataatt
    41821 gattccaatt ggtaaagagt ttagagtact ataattacct gaagaaataa gtaataattg
    41881 ctctagagcc ataattatta caagtaatgt acaaattatc ttctcaactg tatagctata
    41941 aggtaaaata tttttggtta gttttggaca atagtataaa aataatccaa atataaagag
    42001 acttacaaat tgttcactag aaaaaagaaa aattttaaac atataacatt ctctcctata
    42061 ataaatttaa tatgtatcta tataaatata ttcattatat ctcaatttaa tatataaaaa
    42121 agataataat agttagattt tttatatgaa ttttaatagg taaaatatcc tattgttttg
    42181 tagatatatc ctaattatgt caaaaaataa aaaaattttt atttattaga gtttttggaa
    42241 ataataataa cagatattat aattaattaa cagtttttgt aagtatttga caatagagag
    42301 ggtgtaacaa atgggaataa tagagtcagc atcaaaattg gctgagatgg ttcatttatt
    42361 ggctgttgaa aaggggataa ctgatataga agcatgggat gaagctgtga aagaatacag
    42421 caaaatttat gaagaaagaa gaaatgaata gaatgataaa aaatgaaaac ttgatgataa
    42481 aaaataactt tatttaaaga aattcaattt ttatagaagt attaaaaata atataaataa
    42541 aaagatggta tttcaaatgt aagtaaaaaa tcattttggg ataccatcct tattatttat
    42601 tttaataata tataagtaat aaatcttact tatccatatt ttttatgtaa cctctttttt
    42661 ctaacttttt tatatgctcc ttacgtattt catcaagatt aataccatat tgttcttcta
    42721 gcttaaacat catagtaaca catgtctgcg ctacatccat tagttcctta gcagttttct
    42781 ttataatatc aacttcatct aaatcattat tttctccact tagatttctg aatttaccta
    42841 tacattcagc taattcacct tgttcttcac ccattttgat aaatgtagat tctagtgttg
    42901 ggtctaaatt attaagctct ggtaaagata tagtttttag tttcataaaa gctcctttca
    42961 agttattcgt atttatacta aaacatcata acaaaaataa tactaaaaaa aaactttaaa
    43021 ttcaacataa aaacttacat atataataaa aatcatgaaa taaatctata tttgtcttaa
    43081 tacagcctca tataaaactt ctccaatagg tttatttatt acaagagaag ctgacttatc
    43141 atagactgta tttgctttat taataagagc aatataatca cctttgtaat aatctataaa
    43201 acttgctgct gggtacacag ctaatgatgt acctcctata atcaataaat cagcattact
    43261 tatagcagaa attgttttag taatgacatc actgtcaaga gcttcctcat aaagtactac
    43321 atctggtttt actatagaac cacagttgtc acagtaagga atattacctc ccagatttaa
    43381 catagattct aagtcaaaaa acttaccaca tttagtacaa taatttctat gtacagaacc
    43441 atgaagttct agcacatttt tacttccagc catttgatga agtccatcaa tattttgagt
    43501 tatgacagct ttaagcttac ccatttcttc gagttttgct aaagctatat gagcattatt
    43561 gggttttgca ttaggatata ttaatttatc cttataaaat tcaaaaaatt cttctggata
    43621 tctaacaaaa aaagtatgtg aaactaattg ttcagcagta aattgtttat tgagtttttg
    43681 gctaaataat ccagtagaac ttctaaagtc tggaatatta gattcagttg aaacacctgc
    43741 tccaccaaaa aatacaatat tattgtgatt agctattaag tcttttaagc tatttgcatc
    43801 catagttaca cctccaaatt attatatata aattgtacta catattaggt cgtttatgtt
    43861 ttattaattt aaataaatat atagtttaat aagttaaata aagggaatat aaataaaatg
    43921 tatacagagt ataaatagac aatatttact aaaataacaa gttgtcgtat aattatatta
    43981 aaattgtttt agtgtataag aagggcgggg gaagtacgtg aagaaaaaag cagctttagc
    44041 aacattggct atgttaccct taggtgtggt aaatgcacat gctgatggag atataggtat
    44101 agtgactata aattatttaa atgtaagaaa tgaaccaact gccgaaagta gcatagcctt
    44161 tgttgctaaa aaagatgata aagttttgat taaagactct tctaatggat ggtataaaat
    44221 aaaagctgaa tctggacaag aaggttgggc ttcatcaaaa tatatagcaa aatcaaatag
    44281 tgactcttta agaacgtcta caaataaaga aaaacaagtt atttcaaata gtttaaatat
    44341 gagaaatgga gcaggtacaa gttacagagt aataactgta ctaaaaaaag gtcaaaaagt
    44401 agaagtaata tcagagagta atggatggtc taaaatcaaa tatgatggaa gactaggata
    44461 tgtatctagc tcttatttag gagacgtttc aaactcaact aataaatcaa aaactaaaca
    44521 agtgaataca acttcactaa atgtaaggag tggaccaaat acaagttatg gtttgttagg
    44581 caagttgcca aaaggaagta aagtagaagt aatatcagaa agtaatggat ggtccaaaat
    44641 aaagtataat ggaaaagatg cctatgtatc tagtatgtac ttatcggatg taagtcaaag
    44701 taattcagac aattctagtc aaagcaatga taaaaagaat actgataagg ttgtaaatac
    44761 agcttcttta aatgtaagaa gtggaccagg ttctacatat agtaagttgg gaaaagttta
    44821 taaaggaagt aaagtaactg tactatcaga aagtagtgga tgggctaaga ttaattttaa
    44881 caataaagaa gcatttgtag taggcaatta tttatctacc tcagcagata cttcaaataa
    44941 taactcaaat agtaactctg ataacagttc taatagtaat ggtaataatt cttcatcatc
    45001 aggccaagta aatggtatgt caggtataag tggggctaaa attgattata aatctttgag
    45061 ttatacttta gaatctcata taagtaaaca agttgagaaa gcagcatcag gaggaaatgt
    45121 gatagctcca agtaatagga aaagtactcc aagtcctgag tttagtactt tttcagctca
    45181 aagaacaagc tcatttgtaa atgcaagttc aagtgatata gaatattatt taaatcctaa
    45241 gaattttaca aatactacta aaggtatgat gcagttttta aagattaaca gttatagaga
    45301 tggtatttca gaatcaagtc ttaactcgta tcttaatggt ttatcttcta gtgtatttaa
    45361 aaaccaggga gcagccttta taaatgctgc caagaagtat aatatagatg ttgtatatct
    45421 agtatctcat gctatgtggg aaactgctta tggtaagtct acacttgccc aaggtcaaac
    45481 attaacatct tataagggac aacctcttag taagccagtt aaagtataca atttttttgg
    45541 tataggagca atagataaaa gtgctaatgt ttcaggtgca gaagcagcgt attccaatgg
    45601 ctggactagt gtagaagcta caatagatgg ctctgcaaag tggatatcac aaaattacgt
    45661 aaatagttct aagtacaatc agaatactat ctacaagatg aagtggaatt atgattatac
    45721 atggcatcag tatgcaactg atgtaaactg ggctaatgga atttctggaa ttatggaaaa
    45781 tttaattggt ctttatggag gaggaagtag tttggttttt gaagttcctc aatataagta
    45841 atacataaat aaaaaatata gactatgggt agttttcatt ttatttaaat tatctatagt
    45901 ctttttgtat ataatttata atctgtaaat ttaatgttaa caaaactttt atgttataat
    45961 attaattcta agtaaatata agtgagtaat aggtagaaat agataaaaag tggtaataat
    46021 atatataaag ataattttga gaaaggagga tatatatgga aagtattaag gaatatttgg
    46081 ataagttaga aattaataat agtggtcttg gaaagcaact taaagaagtt tatataaata
    46141 gagtagttta ttttaaggaa gataagatag tttattttta tctaacttct aaagatattg
    46201 tttctcatga acttttagat aaatttaaag aagaattgat gtataaactc gattatttta
    46261 aagatatgag gatgaagata agatttactg gtcttgaaag aaaatccaat aaagatgtta
    46321 taaaaaaata ttggaataat atattgtata tattaaagta tctttgtcca tctattgcag
    46381 ggtggtataa acaagtagaa tttctttgtt tagaagaaga attaaaaata aaacttccaa
    46441 aaggtatatt ttatgagcga ttaatgaaaa aaaatgtaac ttatgtactt aaaaccgtgc
    46501 ttagcgaaga gttgggatta gatttaaata taacaataga aaaagctgtt gatgaaaagg
    46561 taaataaaga aagactcata agaatcaatg atagagaaat ggaagaaaaa attagggctt
    46621 tagaaattgg aaaagttaat aactgtgaag aaaatgaaga ggaaagctat gtcattaagt
    46681 ctgatgtaga tgaaaattta atctatggag ataatgcaaa tgctatgatt gaaaatatag
    46741 ttgaattaaa tgcatcttct ggaacagttg cagttgttgg agatatattt gatgttgaca
    46801 ctaaagaact taaaaacgga aaaatactta tgatagcttc aattacagat tatactagct
    46861 caataagctg taaacttttt ttaacagata caaataagga tggagtactt gaaaatgtta
    46921 aaaaaggagc atacttaaaa atcaagggtg acatagttta tgatacatat caaagagaaa
    46981 ttagtatgat gattagtggg ataagaaaag aaactagaat agagagagaa gataaatcag
    47041 aggaaaaaag agtagaactc catgcacata cacaaatgtc ttctatggat gctatttgtt
    47101 ctgttaaaaa gcttgtagag cgtgcagcca aatggggtca ttctgctatt gctataacag
    47161 accatggtgt tgtacaagga tttccagatg ctatgaatgc aggtaagtca aataatataa
    47221 aaatacttta tggagtagaa gggtacctgg ttgaagatga ttctctcatt atagaggatg
    47281 caaatgataa agaactttcg cagacatttg ttgtatttga catagagaca actggctttt
    47341 ctaatacaaa tgataaaata actgagattg gtgctgtaaa aatagaaaac tttaagattg
    47401 tagatagatt tagtgagtta ataaatcctg aaaaagatat ttcttataag atacaggaat
    47461 tgacaggaat aactaatgaa ttaattaaag acaagccaac cattgaagaa gttcttccta
    47521 agtttatgga atttgttgga gatagtgtat tagtagcaca taatgcagag tttgatactg
    47581 gatttatatc gcaaaaatgt agggaacaag gcttgttata taataacaaa aaagtagata
    47641 cacttatgtt agcaagagta atgttgccaa acttaaaaag acatagatta aatgtcgttg
    47701 caaaagaact tggtattcct cttttaaatc accatagagc agtggatgat gctgaagcta
    47761 cagcacttat atttaataag tttttgcaga tgcttacaga aaaaggtgca aaaactctaa
    47821 gtgatgtaaa caatatactt ggaaagattg attacacaaa gcttagcaca aatcatataa
    47881 ccttgatagc aaaaaattct ataggtataa aaaatttata taaaataatt tcagatgcac
    47941 atgtaaatca tttttttaga gcaccaagaa tccttagaag tgtattaaat gaatataaag
    48001 aaggattaat aattggttcg gcttgtgaag ctggggtggt ttttcaagct gtaaagaaaa
    48061 acgtgtctga tgaggaaatg aaaaaaataa tagatttata cgattatatt gaagtaatgc
    48121 ctatagataa taatagattt atgattgata aaggagaagt aaaggacgaa gaagagttaa
    48181 gggagttgaa taggaaacta attgatacag ccaaaaaatt tgacaaaatt ccagtagcta
    48241 ctggggatgt tcatttttta gacaaacatg aagctgtctt aagaaaagtt cttaaatatt
    48301 ctcaaggttt taaagtagat gaagaagaaa cttatttaca ttttagaaca acagatgaaa
    48361 tgttggaaga gtttagttat cttggagaag aattggcata tgaagttgta gttgaaaata
    48421 gtaatctgat tgccgatatg gttgaagata taaaacctat accagatgat acttatcctc
    48481 caattataga gggctcagat gtggagttaa gagaaatgtg ttacaaaaaa gccaaacgaa
    48541 tctatggaga cccaatacca gaaatagttc aaaaaagact ggatagagaa ttaaattcaa
    48601 taataagcaa tgggtatgca gttatgtata ttatagctca aaaactggtt gcaaaatctc
    48661 tttcagatgg atatttagtt ggttcaagag gctcagttgg ctcatctttt gcagctacta
    48721 tgagtgatat aacagaagtt aatcctcttc cagcacatta tatttgtgaa aacgaagact
    48781 gtaaatattc attcttttat gaaataggtg aatggggctc aggtgttgat ttaccagaca
    48841 aggattgtcc aaagtgtgga agaaaacttg ctaaaaacgg tcatgatatt ccatttgagg
    48901 ttttcttagg ttttgaaggt gataaggaac cagatataga cttaaatttc tcaggaaatt
    48961 atcagccaac aattcataaa tacacagaag aactatttgg agaggggtat gtatataggg
    49021 ctgggactat aggtactgta gcagaaaaaa cagcttttgg atacgcaaga aaatatgtgg
    49081 aagaaaataa tataagtgta ccaaatgcag aagtacttag gctttcaaat ggatgtactg
    49141 gagtaaaaag aacctcagga caacatcctg gtggagttat ggttatacca gactataaag
    49201 atgtatacga ttttacacct atacagtatc cagcaaatga tacttcttgt ggtgttataa
    49261 ctactcactt tgattatcat tcaataagtg gtagaatact taaacttgat atacttggac
    49321 acgacggtcc taccattata aggatgcttg aagatatgac tggaataaat ataacagaaa
    49381 ttcctttaga tgataaagag actatgtctc tatttacatc tacagaagca ttaggtgtta
    49441 caccagaaga aataaactgt cctataggtt gccttgctgt accagagttt ggaactaagt
    49501 ttgtacgaca aatgttactt gacacaaaac ctactacatt tgcagaactt gtacgtatat
    49561 ctggtctctc tcatgggaca gatgtttggg taaataatgc acaagactta gttagagaag
    49621 atatagttgg attaaaagat gttatatcta cacgagatga cataatgaac tatttaatat
    49681 ttaaaggatt acctcccaaa atgagcttta caataatgga gagtgtaaga aaaggtaaag
    49741 gtttaaaacc agaacacatt gaagaaatga aaaaaaataa tgttccagaa tggtatatag
    49801 gctcatgtaa aaagattaag tacatgttcc caaaagcaca tgctgttgct tatgtaatga
    49861 catcatttag aattgcatat tgtaaagttc actatccaga agcattttat gcgacttatt
    49921 ttacgacaaa agttgaggat tttgatgcag atttaattgt taaaggatta gatgctataa
    49981 aatcaaaaat aaatgaaatt gagtcactgg gaaatgatgc aaccacaaaa gaaaaaggta
    50041 tgttgaccgt tttagaagtt gctcttgaaa tgtatgctag aggaataaaa ttattaccag
    50101 ttgatatata taaatcagat gcaactgagt ttatagttgt aggagaaaaa acattacttc
    50161 caccaatggc agcaatacaa ggtcttggag gaaatgcggc tataaacatc caaaatgaaa
    50221 gaaaaaatgg tgaatttata tcaaaagaag atttgagaaa gagaacaaaa atatcaaaaa
    50281 cggtaataga aactcttact aatcatggtt cacttgagaa tatgagtgag agaaatcaat
    50341 tgtcattgtt ttaagttagc ccaaaaagtt gcaaataaaa gaattttatg atatcatagt
    50401 atagaaaata gtatactttg cagaaactaa gagtgagaca gctgttctca ctcttttatt
    50461 ttaaatatat tattaaaaag ctggtttatt tttattaaat atattttatt taagattata
    50521 taaactggtc atttacaatt gaataggagg aactttaatg aaaaaaaatt tagaagctac
    50581 aattgaggag attgtaacta aaataacgga tgaacatgga tttgaaatgg ttgatgttga
    50641 atatgtaaaa gaagcaggag aatactactt aagagtatac atagataaag aagaaggaat
    50701 atctttaaat gaatgtgaat tagttagtag agaattaagt ccaatactag atgaaaaaga
    50761 ccctataaaa gaaaattact ttttagaggt atcttctcca gggcttgata gagcattaaa
    50821 aaaagacaga gattttgtta gatatcaagg tagagatgta gatttaaaat tatataaacc
    50881 attaaatgga tgtaagcaat ttgaaggtga acttgtagga ttaacagaag ataataatat
    50941 aaaaatcatt gcaaatggta aggaaatgga atttaataga aaagatgttg ctattgtcag
    51001 attagcaata aagttttagt ggtatattaa atagggagga tataaaaaat gaatcatgaa
    51061 tttatggaag cattagatga attagtagtt gacagaggta tagataaaga aatacttata
    51121 gatactatag aacaagctct tttaactgca tacaagaaaa actttggttc agcacaaaat
    51181 gttagagttg aatttgatag agacaaggga gatataaaag tattttctca aagagttgtt
    51241 gttgatgaat cagatttata tgatactttc ttagaaatag agttagcaga tgctagagag
    51301 ataaatccta attatgaatt aggtgatatt atagaacatg aagttacacc taaaaacttt
    51361 ggaagaattg cagctcaaac agcaaagcaa gtagttgttc aaagaataag ggaagctgag
    51421 agagaaatag tgtataatga atttatagaa aaagaaaatg aaatagttac aggagaggtt
    51481 gctagagcaa ataaaaatgt tgtttatgta aatcttggaa gaatagaagg tataatgact
    51541 caaagtgaac aaataccatt tgagaattat aaagcaggtc aaaaaataaa attctatata
    51601 cttgaagtta aaaagactaa caaaggacct caaatagttg tatcaagaag tcatccgggt
    51661 ctagtaaaga gattgtttga agaagaagtt cctgaaatat atgaaggaat agttcaaata
    51721 aaatctgtag caagagaagc agggtcaaga actaaaatgg cagtaagatc tatagatgaa
    51781 aaaatagacc caattggagc ttgtgttgga ccaaaaggac ttagagtaaa aaatatagtg
    51841 gatgaacttg gcgatgagaa aatagatata ataaaatata gtgaagaccc agcagagttt
    51901 atatcagcag cacttagccc atcaaaggtt gttagagtag atatagatga agatgaaaag
    51961 tctgccttag taatcgttcc agattatcag ttatcgcttg ctataggtaa agaaggtcaa
    52021 aatgcaagac tagcagcaaa attgactaac tggaaaatag atattaagag cgaatctcaa
    52081 gcagaacaag aaatgttaaa tcttgaaaat tccaaagaaa tagaagctga ggatgttgaa
    52141 gctgaattta tcgaccaaga agagttagat acttcagagg ataattttag tgaagaagag
    52201 taaggaggga tgattttgaa aaaagtaaaa aaagtgcctc aaagaaaatg catagcatgt
    52261 caagatagag actctaaaaa agagcttata agaatagtta aaaataaaga aggaaaaata
    52321 tttttagacc caacaggaag agccaatgga agaggagcat atgtatgtaa aagctctgaa
    52381 tgtctaaaaa aagctataaa gagtaaagca ttaaataaag cttttaaaat agatgttcct
    52441 gatgaagtct atgataatct acttatggag ttagaagaat atgaaaaata ataaagaaaa
    52501 agtactttcg ttcttagggc tcgcaactcg tgcagggaaa atagtgtctg gtgatgattc
    52561 aactttgctt gaccttaaaa aaggaaaagt aaatttgatt ttaatcgcag aagatgcatc
    52621 aaataatact aaaaaacttt ttaaagataa atcaactttt agaaatattc catatctatt
    52681 tttttcaaca aaagaggaaa taggatttgc aataggaaaa tctccaagag cagttgtagg
    52741 aataaaagat gaaaattttt ctaaaaagat aattgaacta atagaaattt aaaataaaat
    52801 agggggtgat cttgtgtcaa aaacaagagt ataccaaata gcagaagaat taaatatatc
    52861 aaatgaagag cttataaata agttagctga attagatata aatgtaactg acaaagattc
    52921 agtattagaa ggtgaagagt tagaattagc acttgaaatg ttaggtgaag atttatctca
    52981 agaaaatgga aatgttatag aaatagatgg aaagttgaca gtacaagtat tagcaacgaa
    53041 attagataaa tctccatcag aaataattat gaagcttatg aaaatgggaa ctatggcaac
    53101 aataaatcaa gagataagtt ttgagattgc tgctttggct gccaaagact atggatttga
    53161 attaacagta gctgaaagtg atgatacaga agctttagaa atagaagctt taatggaaat
    53221 tgaagaagac aaagaagagg atttaaaacc tagaccacca gttgttacag ttatgggaca
    53281 tgttgaccat ggtaagacat ctttactaga tgcaataaga aaaactgatg tgatctcagg
    53341 tgaagctgga ggaataactc agcatatagg tgcttcagaa gttaaaatca atggacacaa
    53401 aatagtattt ttagatactc caggacatga ggctttcaca tcaatgagag caagaggagc
    53461 acaagttaca gatatagcaa tattagtagt tgcagcagat gatggtataa tgcctcaaac
    53521 agttgaagct ataaaccatg ctaaagcagc aggtgtacct ttaatagttg ctattaataa
    53581 aatagataaa ccaggtgcaa acccagataa ggttaaacag gaattagcag accaaggatt
    53641 attagttgaa gactggggtg gagaagtaat agcagttcca gtatcagcta agaaaaaaga
    53701 aggaatagat acacttttag aaatggtatt attggttgct gaaatggaag agttaagagc
    53761 taatccaaat aaaagagcag taggaactgt tatagaagct gagcttgata aaggaagagg
    53821 gccagttgca acagtacttg ttcaaggtgg tactttaaca gtaggtgacc caatcgttgc
    53881 aggtgttgca tgtggtaaag ttagagcaat gataaatgca aaaggtaaga gagtaaaaac
    53941 agcaggacca tctacagcag tcgagatatt aggtctatca gaagttccac aaggaggaga
    54001 ccaatttgtc gaagttccaa ctgataagat tgctagaagt gtagcagcaa gaagacaaca
    54061 aatagttaga gatgaaatgt taaaatcaac tcaaagatta tcactagatg ctctatttag
    54121 tcaaatgagt gaaggttcta taaaagacct taatatagtt ataaaagcag atgtacaagg
    54181 ttcagtacag gcagttaaac aatctttaga aaaattatca aatgaagaag ttcaagttaa
    54241 agtaatacat ggtggtgttg gagctgtaac agaatcagac atattacttg cagcagcttc
    54301 taacgcaata ataataggat tcaatgtaag accagttcca ggtgcagaat ctctaggaga
    54361 aaaagaaaat gttgatataa gaacatatac tataatatac aaggcaatag aagatataca
    54421 agcggctatg actggtatgt tagaccctga atatgttgat gaagaaacag gaaaagctga
    54481 aataagagaa atatataaaa tatctggtgt tggaacagta gctggatgtt atgtgactaa
    54541 tggtaagata tttagaaatt gtaaagtaag attagttaga gatagcataa taatacatga
    54601 aggtgagctt gcagcactta agagatttaa agatgatgta aaagaagtta attctggata
    54661 cgaatgtggt atgagttttg taaattataa cgacataaaa gaaggcgata tagtagaagc
    54721 ttatataact aaagaagtag aaagaaaatt ataaattcgt atcaaagaaa atcagttagt
    54781 aaagaaggtc gatggtatgg catcatataa tagaacaaga aggatagcag aagaaataag
    54841 aaaagtcgta agtactatgc ttataaatgg agtaaaagac cctagaataa catctatggt
    54901 gagtgtaact gatgtagaag ttacaaatga cctaagatat gcttatgtat atgtaagtat
    54961 tctaggcgga gatgaagaat ccacactaac aggtcttaaa agtgctggag gatatataag
    55021 aagagaagta ggaaaaaata taaaacttag atatattcct gagatagtat ttaaacttga
    55081 tgattcaata gaaaaaggaa tgtatatgga tagccttata aaaagagtta atgaaaaaaa
    55141 tgctcaacaa aatgaggatg aaaattatga tgaataatat ggatagtatt ataaactata
    55201 tttatgaggg tgactatttt atagttacct ctcatatcag tccagatgga gacaatattg
    55261 gttctacact ttcaatgtat tatacattga agaatttagg aaaaaatgta tattatgtac
    55321 ttgatgatga agctccatta aatttaaggt ttttggttga aggtgtaaaa atctataagt
    55381 ctaatgaatt taatatgaag aactattctc taattgcact tgattgtggg gataagatga
    55441 gaatatgtgt gagtgatgaa atcaaaaata atgcatcaaa attaatctgt attgaccatc
    55501 atgctagtaa tgattcatat ggagatttaa attatgtaga tattgatgca tcttctacat
    55561 gtgaattagt ttataattta ttagttagat ttcagcaaat aaaagatatt aatattataa
    55621 atgaagatat agcaacatgt ttatatacag gtcttgtaac tgatactgga aacttttcgt
    55681 attcaaatgt acatgcaagt agctttgaaa tggcaaaaaa tttacttgtt ttaggtgcac
    55741 aaaaaaatac tataatacaa aatatatatc aaagcaattc ttctggttat tacaaattat
    55801 taggagaagc tctaaagggt ttggagatat ttgattaaaa aatagttacg cacactcgtg
    55861 actttagtca tgagttagta gctaaaatag tcagcatata gggaaactta tatgtagaga
    55921 taggata
//
LOCUS       DAEMGG010000011        52111 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEMGG010000011 DAEMGG010000000
VERSION     DAEMGG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.011"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(223..1266)
                     /locus_tag="KPK24_000684"
     CDS             complement(223..1266)
                     /locus_tag="KPK24_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG2298391.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(1287..1733)
                     /locus_tag="KPK24_000685"
     CDS             complement(1287..1733)
                     /locus_tag="KPK24_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG2298392.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(1911..3491)
                     /locus_tag="KPK24_000686"
     CDS             complement(1911..3491)
                     /locus_tag="KPK24_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBG2298393.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            4290..5192
                     /locus_tag="KPK24_000687"
     CDS             4290..5192
                     /locus_tag="KPK24_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298394.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            5179..5934
                     /locus_tag="KPK24_000688"
     CDS             5179..5934
                     /locus_tag="KPK24_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2298395.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            6031..7068
                     /locus_tag="KPK24_000689"
     CDS             6031..7068
                     /locus_tag="KPK24_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2298396.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            7877..8779
                     /locus_tag="KPK24_000690"
     CDS             7877..8779
                     /locus_tag="KPK24_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298397.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIISFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            8766..9521
                     /locus_tag="KPK24_000691"
     CDS             8766..9521
                     /locus_tag="KPK24_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2298398.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            9652..10674
                     /locus_tag="KPK24_000692"
     CDS             9652..10674
                     /locus_tag="KPK24_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2298399.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(10837..11394)
                     /locus_tag="KPK24_000693"
     CDS             complement(10837..11394)
                     /locus_tag="KPK24_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG2298400.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(11400..12476)
                     /locus_tag="KPK24_000694"
     CDS             complement(11400..12476)
                     /locus_tag="KPK24_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG2298401.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(12477..13160)
                     /gene="modB"
                     /locus_tag="KPK24_000695"
     CDS             complement(12477..13160)
                     /gene="modB"
                     /locus_tag="KPK24_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG2298402.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(13309..14121)
                     /gene="modA"
                     /locus_tag="KPK24_000696"
     CDS             complement(13309..14121)
                     /gene="modA"
                     /locus_tag="KPK24_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2298403.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDNGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            14314..15243
                     /locus_tag="KPK24_000697"
     CDS             14314..15243
                     /locus_tag="KPK24_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298404.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(15404..15853)
                     /locus_tag="KPK24_000698"
     CDS             complement(15404..15853)
                     /locus_tag="KPK24_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298405.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(16056..17036)
                     /locus_tag="KPK24_000699"
     CDS             complement(16056..17036)
                     /locus_tag="KPK24_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2298406.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            complement(17241..18101)
                     /locus_tag="KPK24_000700"
     CDS             complement(17241..18101)
                     /locus_tag="KPK24_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG2298407.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(18418..19746)
                     /locus_tag="KPK24_000701"
     CDS             complement(18418..19746)
                     /locus_tag="KPK24_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2298408.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(19894..20223)
                     /locus_tag="KPK24_000702"
     CDS             complement(19894..20223)
                     /locus_tag="KPK24_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2298409.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(20247..21536)
                     /locus_tag="KPK24_000703"
     CDS             complement(20247..21536)
                     /locus_tag="KPK24_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2298410.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(21552..21878)
                     /locus_tag="KPK24_000704"
     CDS             complement(21552..21878)
                     /locus_tag="KPK24_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2298411.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(22013..22642)
                     /locus_tag="KPK24_000705"
     CDS             complement(22013..22642)
                     /locus_tag="KPK24_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298412.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(22858..25515)
                     /locus_tag="KPK24_000706"
     CDS             complement(22858..25515)
                     /locus_tag="KPK24_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2298413.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKHYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(25874..26818)
                     /locus_tag="KPK24_000707"
     CDS             complement(25874..26818)
                     /locus_tag="KPK24_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298414.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(26811..27836)
                     /locus_tag="KPK24_000708"
     CDS             complement(26811..27836)
                     /locus_tag="KPK24_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298415.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(27911..29479)
                     /locus_tag="KPK24_000709"
     CDS             complement(27911..29479)
                     /locus_tag="KPK24_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2298416.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGVFWNIADWTIE"
     gene            complement(29592..30503)
                     /locus_tag="KPK24_000710"
     CDS             complement(29592..30503)
                     /locus_tag="KPK24_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298417.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(30487..31473)
                     /locus_tag="KPK24_000711"
     CDS             complement(30487..31473)
                     /locus_tag="KPK24_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298418.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            31833..32234
                     /locus_tag="KPK24_000712"
     CDS             31833..32234
                     /locus_tag="KPK24_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2298419.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            32237..32635
                     /locus_tag="KPK24_000713"
     CDS             32237..32635
                     /locus_tag="KPK24_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG2298420.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(33085..33309)
                     /locus_tag="KPK24_000714"
     CDS             complement(33085..33309)
                     /locus_tag="KPK24_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG2298421.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(33344..34711)
                     /locus_tag="KPK24_000715"
     CDS             complement(33344..34711)
                     /locus_tag="KPK24_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2298422.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTAMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(34795..36330)
                     /locus_tag="KPK24_000716"
     CDS             complement(34795..36330)
                     /locus_tag="KPK24_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298423.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDAILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(36353..36787)
                     /locus_tag="KPK24_000717"
     CDS             complement(36353..36787)
                     /locus_tag="KPK24_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298424.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(37047..37958)
                     /locus_tag="KPK24_000718"
     CDS             complement(37047..37958)
                     /locus_tag="KPK24_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG2298425.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            38254..38607
                     /locus_tag="KPK24_000719"
     CDS             38254..38607
                     /locus_tag="KPK24_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG2298426.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            complement(38826..39767)
                     /locus_tag="KPK24_000720"
     CDS             complement(38826..39767)
                     /locus_tag="KPK24_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG2298427.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(39868..41103)
                     /locus_tag="KPK24_000721"
     CDS             complement(39868..41103)
                     /locus_tag="KPK24_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG2298428.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSQSKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLI"
     gene            complement(41147..42001)
                     /locus_tag="KPK24_000722"
     CDS             complement(41147..42001)
                     /locus_tag="KPK24_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298429.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(42129..43304)
                     /locus_tag="KPK24_000723"
     CDS             complement(42129..43304)
                     /locus_tag="KPK24_000723"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG2298430.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(43403..44287)
                     /locus_tag="KPK24_000724"
     CDS             complement(43403..44287)
                     /locus_tag="KPK24_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG2298431.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            complement(44519..45205)
                     /locus_tag="KPK24_000725"
     CDS             complement(44519..45205)
                     /locus_tag="KPK24_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2298432.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            complement(45233..45544)
                     /locus_tag="KPK24_000726"
     CDS             complement(45233..45544)
                     /locus_tag="KPK24_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2298433.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            complement(45733..46254)
                     /locus_tag="KPK24_000727"
     CDS             complement(45733..46254)
                     /locus_tag="KPK24_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2298434.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            complement(46351..46845)
                     /locus_tag="KPK24_000728"
     CDS             complement(46351..46845)
                     /locus_tag="KPK24_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2298435.1"
                     /translation="MIRKANMNDLESIMQIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            complement(46879..47352)
                     /locus_tag="KPK24_000729"
     CDS             complement(46879..47352)
                     /locus_tag="KPK24_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2298436.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            complement(47678..48673)
                     /locus_tag="KPK24_000730"
     CDS             complement(47678..48673)
                     /locus_tag="KPK24_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG2298437.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            complement(48695..50620)
                     /locus_tag="KPK24_000731"
     CDS             complement(48695..50620)
                     /locus_tag="KPK24_000731"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG2298438.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREEAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            complement(50645..51838)
                     /gene="nifV"
                     /locus_tag="KPK24_000732"
     CDS             complement(50645..51838)
                     /gene="nifV"
                     /locus_tag="KPK24_000732"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG2298439.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
BASE COUNT        16965 a         9089 c         5651 g        20406 t
ORIGIN      
        1 ctatatgttt tatattatat tatataacac tttttacatt agtaaaggat aaattgtata
       61 aagattctgt taatttattt ttataatttc atctttctaa taaaaacaaa gaagaagact
      121 taaaaattta taagtcctct tcttttcaac gtgcattttt gtgctatatt taattaattc
      181 gacctttaat ttccatatcc ttcctatgat aacaaaactc ttttattttt ttaatgattc
      241 attatcattt aaaacttctt ttatatcaag tacttctttg ttatcatcac tcataacttc
      301 ctttattcct ttgaatgttc ctaggaaatt aactgcttct gaaggtaaaa ccagttttgt
      361 tgacttacct tcagccattt tttcaagagc ttccatagat tttaatgcta gaatattatt
      421 gtctatatca gcctctttca ttgctgaaaa aactaatttt aaaccttctg ctgttgccat
      481 ttgagttctt tttatcattt cagcttcacc ttgtgcctta gcaatagctg tctgtcttat
      541 agcttcagct tcaccttctg ccactaatat agcagattct ttttcacctt ctgctacacg
      601 taccatagct tcttttttgg cttctgctgt taatattgca gattgttttt caccttctgc
      661 ttgaagtatt gcagctgact tattaccttc tgcttgaagt attgcctctc ttctttctct
      721 ttctgctctc atttgttttt ccattgcaac ttgaatatcc tgaggaggca ttatattttt
      781 taactctact ctatttactt ttatacccca tttatctgtt gcttcatcaa ggatagttct
      841 catttttaca tttattatat ctcttgatgt taatgtttca tctaaatcaa gttcaccaat
      901 tatatttctt agtgttgtag ctgttaaatt ttcaatagca gctattggat tagctatctc
      961 aaacacatat ctaactgggt ctgtaacttt ataatataca actgtatcaa tctgcatagt
     1021 tacattatct ttagttataa ctggttgagg tggaaaatct attacaattt ctctaaggtc
     1081 aattacatat gccattttat ctaaaaatgg tattaagaaa tgtacacctg tttcagctac
     1141 tttttgaaac ttaccaagtc tcattattat acctacttta gattgtttta taactcttat
     1201 acaagttaaa cttattgcta cgactactac tattaataca atacttaaaa caattttagt
     1261 acccatacta taaatcctcc tttattctat tggtttgtca cattaactgt ggcgtctact
     1321 tttttaacaa ctaattttac cccttcaatt tttaatactt ctactgtaga acctttttct
     1381 atcttttcac catctataga aatagccgtc catgtttcat gttcaactac aactagacct
     1441 gtgttatttg gagaaatctc ttctgtaaca ataccttttt tagacatcat tgcttgtaaa
     1501 ttagtgttag atttatagcc tttatctttt tttactattt ttttagtagc tacgactaac
     1561 attgctatag aaattactgc aaatattaaa atttgtaaga aaatactctc tataaagcta
     1621 cttaaaaata taagtattac agctcctaca ctaaaccata tcaatgttaa gcttggtgtt
     1681 aacatttctg ctattccaaa taagactgct actatcaacc atattaactt catactatct
     1741 accctctttt catatgaatt ataaaaccct atataaatta tatgtttata caatgttttt
     1801 gaatataaat ttaaattaca aaacaataaa ataaattaat taattaatta atagtattat
     1861 atcaacatac tttgttaaat ttgttacaaa gtagtatcaa atatcaccaa ttatatatcc
     1921 aacaactctt tcattgttaa tgaaatgtta cttattaaat ccgtagcatt taccgtatac
     1981 attgctttgc tcagtttatc tcctatatag ccatgtatat atgctccact aactgtagct
     2041 tccaatatat ccataccttg acctataaat gaagtaatca ttccaagcaa acaatcaccc
     2101 attccaccag ttgccataca gctatttcct gtaggattta tatatgtttt tttaccatct
     2161 gttatgacag tttgataacc ttttaaaagc accaccagat tatatttttg agcaaattct
     2221 tttgcaacat ctactctatg ttctcttata tacccaattg aatctcctgt caacctggcc
     2281 atttctccca aatgaggtgt aattacaaat ctatttttcc attctaaaaa cttataacat
     2341 ctgtccttca taacgttcaa tccatctgca tctaatacta ttgggcaatt agaattttct
     2401 actatcttaa gtaatttatc aaatgtttga ctattatctc ccatacctgg accaaaacca
     2461 attgcatcac tagaattaag taatttattt aatctttctt cttcaaaatt taaagtcatt
     2521 gcttctgtaa gtctaataga taccttttct tgcacatctt cactactaat taatgttaca
     2581 agtccactcc cactcttaac agcagattca gttgctatga aacttgcacc ataaaatcct
     2641 tttgaacctg caactataga tacttttcca aaatcacttt taaaaccatg cttatctctt
     2701 cttctaatat tattttttac aaaagtctta tcagttatga actcttcatc atggtatttt
     2761 cttgtaataa aatctggaac acctatcttt tctacaacta catcacctat aagtgcgcta
     2821 gtgtcatagt ttagaaaacc tcttttataa aactcaaagc taatcgtttt gtttgctcta
     2881 atacaagttc ctaatacatt tccattatcg ctatctatac ctgaaggaat atctatagaa
     2941 tacgtatttt tgctattatc gtttactatg tcaataactt ctttaaaaat accagttata
     3001 tcacgtttta atccagtccc aaatatagca tctactacaa catcactttt agatactcta
     3061 tctttaaaaa ttgttaaatt tttatttttt ataatatttt gtttctcaac tttaataatt
     3121 ttatctactt cattttcctc tccataattt ttttcactta aatcattttt attaatattt
     3181 ataatattaa ttcccatatt taaaagtaca ttgtaattta ttttagagca ttcacttagt
     3241 ttttcaagat tacctactaa aaatacgttt acttcttttc catatgtatt tagatgtctt
     3301 gctattccaa gaccatctcc tccattattt ccaacaccac atacaactgt ataactatta
     3361 tacatgtcta tatccatatt tttcattgca gatataaccg cattttccat caatacaatc
     3421 attggaatcc ctacttcttc tacacatgca gtatcaacat ttctgcttaa tttagatgta
     3481 cctattagca taaattatca ctcctataaa gttaaagact tcttaaatac ttaccaaaaa
     3541 tatctattga aatcttttta atcccataaa tatcataaat aaaatcacta aaactaagag
     3601 ttttgaaata atttaaaata acaaaaaatt ttgaattaaa acaattattt aatttattat
     3661 accatataat ttaagttatt atgttgattt aaaacctatt tacactaatt atctaaatat
     3721 ctctttattt ttataatctt ttatgactta tttatatttt tcatttttat aaaaaaacaa
     3781 taattaagaa tataactata ttctacatgt aaaaattttt ttatttaaac tcaattttct
     3841 gataatttga taatttttca aaatttatag ttgactttta ttttttactg tattaaacta
     3901 attactatac aaaacagaaa acaaaatttt caaaattatt aaattaaaag gagaggtaaa
     3961 tatgaagact ttattaaaca aacaagtacg attttttagt agccaattta gttagggttt
     4021 gtacctaagt taagacaaat tgtcataaca tagatacaag ccccttagga ttatgatttt
     4081 taaggattgt atctatgttg gctatagtaa ttgttgttaa agtttttatt ttatcatcat
     4141 aatatagagc cacatggatt atactgtgtg gcttattcga atttttgcca tacagttata
     4201 tatactgtgt ggttttttta tatcaaaatc attttttaaa accaaatttt aaaaaccatt
     4261 taaaatattt tcaaaggggt gttttattta tgtcaggtat tatatctgta atgacgcaaa
     4321 gtttaatttt atcgattatg gcactggggg tttatataac ttacaaaatc ttagattttc
     4381 cagatatgtc tgctgatggt agctatacaa tgggtgcttc tattgtagca ttttctctta
     4441 ctaatggtat ttctcctgta gtagcaactt taatggctat cttatgtggt tgtactgctg
     4501 gtcttgttac agggattctt cacattaagt ttaagatatc taatttactt tctggtattt
     4561 tagttatggg aatgctatac tctatcaatt taagaataat gggaaaatca aatattcctc
     4621 tatttagctt taaacactta ttcaatgggg aaatatctcc tatagtttta gccttagctt
     4681 ttgtattcat ctgtaaagtc cttttagatt tattcctaaa aacaggtctt ggatacactt
     4741 taaaaggtgt tggtgataac tctcaaatga ttaaatctct aggtataaat ataggttcta
     4801 taaaaatact aggacttatg atttcaaatg gacttatagc tttatctggt agtcttatgg
     4861 ctcagttctt gggattctca gatgtaaata tgggtatagg aactttagtt cttggaattg
     4921 cctctatcat aattggtatt acactattta agaaatttac ttttatcaaa gatactactg
     4981 caataattgt tggctctttt atataccaat tcactatata ctttgcaatg agtttaggaa
     5041 tgttatcaac tgacttaaaa ctaataactg ccatagttat aatagctttc ctagctactg
     5101 gtaacttaaa tatttcacta aaaaaaaaca aatgcaaagc tagtaccaaa aataaatcag
     5161 aaaaaagagg tgttgtagat gttacaaatt aaaaatcttt caaagagttt ccctaatcca
     5221 tatggtgaac ctaatactat ctttgaaaat ttatcaatcg atatagaaga tggtgaattt
     5281 gtaagtatca taggtagtaa tggaactggt aaatctactt tattaaatat aatatctggg
     5341 cttataaaag aatcttctgg tcaaattact ttagatagta ctactataac aaatttagct
     5401 gagtataaaa gaactcaaat agtaagtaga gtttttcaag acccaagcct tggaacttgt
     5461 ccttctatga cggttagaga aaatttatct ctagcactta ataaagggaa attattgaac
     5521 ttaaaaaaat gtcttcgtca taaaacgaat gacttggaac acttacttga aggaatttct
     5581 ttggatttaa aaaaatattt agatattaaa gtacaatatc tttcaggggg tcaaagacaa
     5641 tcactatcac taattatgtc tagcttagca agtcctaagg tcttattact agacgaacat
     5701 acagcagcac ttgacccaaa aacatcaaat gaagtaatag agttaacaga taaaatcgta
     5761 agagagaaaa atataactac acttatggtt actcacaact tgaaacacgc tcttcaatat
     5821 ggagataggt tgataatgct tcataagggt gaagttgtat tagatgtaaa agataaagaa
     5881 aaaaaagatt taacagttga agaaatctta gaaaaatttg aatatgcagt ataagaaatt
     5941 aaaatcttag ttttaatcag aaatatttat aatctatcat atgaatatat gaacaaatat
     6001 aattattaaa ttttaaggga ggaaaataaa atgataaaaa gtaaaaaaat attaagttta
     6061 attatagcag gagtacttgg tgtatcaatg cttactggat gttctcaaaa tgatggctcc
     6121 aatgcttcaa atgaaaataa agaaacagat agcaaaaaac aaaaaaatat aggtattact
     6181 caattagtgg aacatccatc tctggataaa gcaaagaaag gattcatcaa agcacttgaa
     6241 gataaaggct ataaagatgg agataatata aaaatagatt tccaaaatgc acaaaatgat
     6301 atgcctacta cacaaagtat tgctagtaag tttgtatccg acaaaaaaga tttgatatat
     6361 gctatatcta ctccatctgc acaagcagct tataatgcta ctaaagatat tccaataata
     6421 atgactgctg ttacagaccc tgtagaagct ggacttgtta aatctcttga aaaaccaggt
     6481 ggaaatgttt ctggtacatc tgattatctt tcaattgata aaacattaga attggttaaa
     6541 acattgactc caaaagcaaa gaaaataggg gttatataca atactagtga agttaattca
     6601 aaaatccaag ttgattctct acatgattat gctaagaaaa ataattatga agtagttgaa
     6661 aaaggaatca gctcttcaag tgaagttaac caagctattt ctagtttagt tggtaaaata
     6721 gatgttttat atgtccctac tgacaattta atagtttctt ctatgccaat agtttctaaa
     6781 gttgctaatg aaaacaagat tcctataata gcctctgaag aaggttctgt atcttctggt
     6841 gctttagctt gttgtggaat agattatgaa aaactaggtt ataaggctgg agaacttgct
     6901 attgaagtat tagaaggaaa atctgttggc gatataccag ttactacatt agatgaaact
     6961 gaaataataa ttaacgaaga tacactaaaa gcacttgaca tgcaaaagtt atcagcagat
     7021 aatataaagt atataaagtc agatgaaaat gcaaaatcta caaaataaaa cttattaatt
     7081 tcaaaaaata ggtaatatga agtaggtaat atgaaaactt tatataataa gtactcttat
     7141 ccaccctaac cctttgaaaa ttgtgcttat tgtagcaatt tgaatattgc atataaagtt
     7201 attcattttc cataaaagtc gtctatattg acgactgatt atgaaagtta tataactttc
     7261 taatcactac ttgagctgtc caaaagaatt ttaatattct ccagacagct tttttttatg
     7321 atatttgatt gatatacatg tctagttatt ccatatttag taaatatttt gataaaattt
     7381 ccacattcac ttataactta ggataacgtt tttttcaaac aatacattaa tttaaaagta
     7441 ttctgagtat acattattta gtattttaat taaatttagc agttgacttt tattattgta
     7501 taatttataa tttataacaa gtcgagaata catataattt tctgactaat taaaatatta
     7561 aaagggagag ataattatga atactttatt aaacaataga aaatattatt attattatag
     7621 ccactatttt tagggtttgt acctacgttt taacatagat acagacccct taagactact
     7681 aaaattttgg gattgtatct atgttggcta tattgtttat aaagttttca tttaactata
     7741 taagagccac atagattaaa ctatgtggct tattcgtgtt tttaccacat agttttgtac
     7801 actatgtggt ttttttatac acaaaattag caaataatta aaattataaa aatattttca
     7861 aaggagtgtt ttatttatgt caggtattat atctgtaatg acgcaaagtt taattttatc
     7921 gattatggca ctgggggttt atataactta caaaatctta gattttccag atatgtctgc
     7981 tgatggtagc tacacaatgg gtgcttctat tgtagcattt tctcttacta atggtatttc
     8041 tcctgtagta gcaactttaa tggctatctt atgtggttgt actgctggtc ttgttacagg
     8101 gattcttcac attaagttta agatatctaa cttactttct ggtattttag ttatgggaat
     8161 gctatactct atcaatttaa gaataatggg aaaatcaaat attcctctat ttagctttaa
     8221 acacttattc aatggggaaa tatctcctat agttttagcc ttagcttttg tattcatctg
     8281 taaagtcctt ttagatttat tcctaaaaac aggtcttgga tacactttaa aaggtgttgg
     8341 tgataactct caaatgatta aatccctagg tataaatatc ggttctataa aaatactagg
     8401 acttatgatt tcaaacggac ttatagcttt atctggtagt cttatggctc agttcttggg
     8461 attctcagat gtaaatatgg gtataggaac tttagttctt ggaattgcct ctatcataat
     8521 tggtattaca ctatttaaga aatttacttt tatcaaagat actactgcaa taattgttgg
     8581 ctcttttata taccaattca ctatatactt tgcaatgagt ttaggaatgt tatcaactga
     8641 cttaaaacta ataactgcca tagttataat atctttccta gctactggta acttaaatat
     8701 ttcactaaaa aaaaacaaat gcaaagctag taccaaaaat aaatcagaaa aaagaggtgt
     8761 tgtagatgtt acaaattaaa aatctttcaa agagtttccc taatccatat ggtgaaccta
     8821 atactatctt tgaaaattta tcaatcgata tagaagatgg tgaatttgta agtatcatag
     8881 gtagtaatgg aactggtaaa tctactttat taaatataat atccgggctt ataaaagaat
     8941 cttctggtca agtattatta aataaacata acttgtctaa tttagctgaa cacagaagaa
     9001 ctcaaatagt aagtagagtt tttcaagacc caagtcttgg aacttgccct tctatgactg
     9061 ttagagaaaa cttgtcttta gctcttaaca aaggaaaact attaaatcta agaaaatgtc
     9121 ttcgttacaa aagagatttc ttagaaaatt tattggaagg tgtatctctt gatttaaaaa
     9181 aataccttga tgttcaagta aaatttttat caggaggtca aagacaatca ttatcactaa
     9241 ttatgtcgtg cttaacaaat ccatcagttt tattacttga tgaacataca gcagcacttg
     9301 acccaaaaac atcaaatgaa gtaattgaat tgactaataa gattgtaagg gaaaaaaata
     9361 taactacact tatggttact cacaacttaa aacacgctct tcattatggt gatagattga
     9421 taatgcttca taagggtgaa gttgtattag atgtaaaagg taaagaaaaa gaacaattaa
     9481 cagttgaaga aatcttagaa aaatttgaat atgcagttta aaataatata tctattgtat
     9541 taaactcggc tttaaaataa agttaaaatt acaataatta tggttacttt gtaataattc
     9601 atatgaatat atgaacatat ataataataa atttttgagg gggatataaa aatgattaat
     9661 aaaaaaagat tggcaagttt gattttagct ggtgctctta gtatatctat gcttacaggg
     9721 tgttcacaag gaggagactc tggtaattca aaacaagaaa gtaattctaa aggtaaagaa
     9781 gttaaaaaaa ttggtataac tcaactggtt gaacatccag cattagatgc cactaggaca
     9841 ggttttgtaa aagcattaga aaagaacgga tttaaagatg gagaaaatat tgatatagac
     9901 ttccaaaatg ctcaaaatga catgcctact acacaaagta ttgcgagtaa atttgcatct
     9961 gataagaagg acttaatatt tgctatatct actccatcag ctcaggcagc atttaatgct
    10021 actaaagata tacctatact tattactgct gtgagtgacc ctgttgctgc tggattagtt
    10081 aaaactcttg aaaagccagg cacaaatgta tctggaacat cagattttgt ttctgtagac
    10141 aaaggtttag aacttcttaa aatatttgct cctaaggcaa aaactatagg agttatgtac
    10201 aatactagtg aagtaaattc caaagttcaa gttgatgctt taaaagaata tgcatctaaa
    10261 aatggattta aagttgttga aaaaggtata actacttcaa atgaagttaa ccaaggtatt
    10321 tctagtttag ttggcaaaat agacgtatta tatgtcccta cagataactt agtggcttct
    10381 tctatgccta tagtctctaa aatagctact gagaataaaa tacctgttat agcagctgaa
    10441 tctggtccag ttgaaaaagg tgctcttgct tgtcaaggta tcaactacga aaaactaggt
    10501 tacaaaacag gtgaaatggc agttaaaatt ttaaatggcg aatcagtatc tgatatgcct
    10561 gtggctacat cagatgatac agatatcatt gttaatgaag atatattaaa agctttaggt
    10621 atggaaaaac cttctaatga aaatatttct tatgtaaaga ctaaacaaga atagaaagat
    10681 attttctata tacgattaat atgcctgttt ctataaatta atttatagaa acaggcatat
    10741 atttttattt taatgtatta tctatttaaa gattatatgt aaatgctctt aatttgtttg
    10801 tataaatatg attgtataaa ctttatacaa tcatatttat tcttctattt tctttattac
    10861 tctacatgga tttccaacag ctacagtatt tggtggtata tcttttgtga ctacacttcc
    10921 tgcacctatt actacattat cacctatagt aattcctggt gttatgatta ctcccccacc
    10981 tatccataca ttatcaccta tttttatagg agaaccatac tctatgcctg aatttctaag
    11041 ttgtgcatca attggatgat aggctgtata gatttgaacg tttggagcta acattacatt
    11101 gtcacctatt tcaattttac atacatctaa aaaaatacaa tcataatttg caaagaaatt
    11161 ttcgcctaca tgtatgttat atccataatc acacctaata ttttgttcta catttatttg
    11221 cttgccaact gaaccaaata gttgtcttaa aatatcttct cttttttcat attcatcctc
    11281 taaagtatta ttaaacaatc tagttaattt tttacaatac tctctttctt ttactaacaa
    11341 ttcatcattt gcatagtatc ctttcccaga caacattttt tctttttcag tcatatagtt
    11401 tactcctttt tgttataatt gtatctaaag caaaataaat attcctgtgg aaaatctaaa
    11461 tatattctgt cacctttctt aaatctcatt ttactttttt ccaattctat ttgaataggt
    11521 acacattcgt tagataaatc agtttttcta acatatatag tatatgtaaa aggattttct
    11581 acaatgttaa tcaattcgaa acataacttt tcatttaaat cttcattaga atccaagacc
    11641 ttaatatgat gctccctgat tcccacatat tctatattat cacctatttc tctatttaat
    11701 gtcaactcgc atccccagtc tgtagcataa atagtatttt tatccagtct atttaatttg
    11761 gatatatttt tacaccctgt tatcttagct tctataaggt actttggaga ttcaaagagc
    11821 tcttttttag gtctctttgg aagaccttct cctttgttgt acactataat atcatcacaa
    11881 actcggtaag cttcttcaat atcatgagta acaaatagta tatctccctt gtaatctttt
    11941 aacatattca taagttcttt ttccatattg cttcttaaat gatggtccag tgctgagaaa
    12001 ggttcatcta aaagtaatat atctggagat gttgccaatg ctctagctag tgctaccctt
    12061 tgttgttgac caccagataa ttgccaaggg tatctcccct caaacccatt taatttaagt
    12121 atatctaagt accttgctac tatttccttt ttttcactct tacttagttt taaaagccca
    12181 agttctatat tttgacttac agtcatatgt ggaaacaaag catagttttg aaataaaaat
    12241 cctacctttc tatcttgtgt tgataaattt atcttctttt cagaatcaaa tagaactcta
    12301 tcatttaata caattttacc tcttgttggt ttctcaagcc ctgctataca ttttaatgtc
    12361 atactttttc cagaaccaga ttctcccaaa aaccccagag tacctttttc ttgtttaatt
    12421 tctaccttta gtttaaaaga ggataaatct ttttcaatat ccacgtataa gctcaactat
    12481 ccacacctct ttcctataat tttctgctga aattccgacc aataatttag taataaaatc
    12541 attgctatag atattgctac aataatcata acccacatca tagctttatt catatctccg
    12601 ccttcaacag caaaaaatat agctattggc atagtttgag tttttccagg tatatttcca
    12661 gctatcataa gtgtcgctcc aaattcgcca agagctctgg caaacgacag caccaatcca
    12721 ccaattattc caggccatgc taatggaact gcaatcttaa aaaatacttt ccattcatta
    12781 agacctaatg ttctagcagc tgacaacata ttattatcaa tctgttcaaa agctgacctt
    12841 gaagttctat acatcatagg aaaagacacc actactgctg aaattacagt tgctgtccaa
    12901 ctaaatatta aagtcctatc gaaatttaat aaaaactttc ctataaatcc attcttccca
    12961 catagtaata ataaaaagaa acctacaact gttggtggca aaattagtgg aagtgtaaat
    13021 agtccatcta taagaccctt ccacttacct ctatagttag ccataatata tgacacaaat
    13081 attcctatta caaaagttat tattgttgac aaaatagatg tcttaagtga tatccataag
    13141 ggtgaccaat ctgttcccat ctttccctcc ttaattttaa atggaaatag tgaaactaat
    13201 aactctatta atttcactat ttccatttta ttataataca caattatttt caattttcat
    13261 ttatttatat ataatttata cttaattaca cataatttat ttctaagttt attctacttt
    13321 tttatatcca aattcttcaa aaatcttttg tcctgactca cttaataaaa attcttcaaa
    13381 tttcttagca gcatctacgt ttttactagc tttaattact gaaactggat atgtaatagg
    13441 cgaatgagtt ttttcgtctg ttttttcaac tacttttatc ttatcattat tgacagtatc
    13501 actgaagtaa acgaatccaa catcagcatt tcctgattgc acccatgcta aaacttcttt
    13561 tacatctttt gcaaatacta gcttatcttt taacttatct tttaaattta aattagttaa
    13621 cacctcatct gcatacttac cagctggaac actttcagct tcaccaacag caattttctt
    13681 cactttatct gtagtcaaat ctttcatacc tgatacacta ctgtcagcag aagaaataag
    13741 aactaaatca ttttttacta aatctttcat agtatcagaa actaataact tctcttcatc
    13801 caaagctttc atttgttttt gacctgctga aataaataaa tcacaaggtg ctccttgttc
    13861 tatttgttgt tgtaatgagc ctgatgctcc atagttaact gtaagtttta catttgaatc
    13921 aactttttta tattcttctt caatctttgc cattgcttct tttaaacttg cagcagccga
    13981 tatatttaac tctactgaat cactactttt agttgttttt tcttgttttc cattatcttt
    14041 tttgtcttca ttactattgc acccaactgt ccccaaagtt aaacatgcaa ctaatcctaa
    14101 tattcccaat atttttttca taacaatctc cctttttgta aatttgatac acattttaac
    14161 atgttttatt atgttttatc atgttttgtt atatatagtt taattatata tacatgataa
    14221 taaattttca agtaatttat ttacactact tttaaatatt ttgctacatt ttcctttttt
    14281 taatgttaaa ataaccttat atggaggtgg attatggact cattatcatc tttgacacct
    14341 gtcgaggtag ctgaattatt aaagataaca aaaaatactg tatatgaatt aatcaaaaga
    14401 ggtgaacttc cttcttataa agtagggaaa aaaataagag ttgatactaa agatgttgaa
    14461 gaatatatca acagccaaaa aactggtaaa gtaaaggata cacatttcaa caataaaaat
    14521 atagatataa ataataattt caaaattatt atttctggtc aagatgtaat attagacatt
    14581 ttagctaggt ctgttgaaaa aaaattggat ggaataagta catttcgctc ttatataggt
    14641 agttataatg ctttgtatga gttatataat ggcagagttt ctatagcatc atcccatcta
    14701 tgggatttcg aaacggatga atataatagc acttttgtta aaaagctatt gccaggaata
    14761 ccatgtgtac ttataaatct agcttataga atgcaaggtt tctatgttgc taaaggaaat
    14821 cctaaagaga taacaacctg ggaggattta acaaaaccaa gtataaccat tgtaaatagg
    14881 gaaaaaggtt ctggaactag ggttttacta gatggtaaac ttagacttct aaactttaat
    14941 ggtaaacata taaaaggtta tgataatgaa gaattatcac atcttggagt tgcaagtact
    15001 gtttctcgtg gaatagcaga tgttggtttg ggaaatgaaa aagctgcttt acaagtaaat
    15061 aatatcgact ttattcctct tcacaaagag cgctatgatt tagttattaa aaaagaagac
    15121 ttacaaaatc cagtttacca aactataatt aacataataa actctcctga gtttaaagct
    15181 gaacttcaag gtcttggcgg atatgattta actgatactg gaaaaactat agcaaaagtt
    15241 taagattaaa atcgcaatct attttataag atttataatt tttcacaaaa tttataactt
    15301 aaacacgaaa taaattgcga ttttagtgtc tcaaaaaatt tatgtctttc attttatcat
    15361 ccaatacaaa atataatata attaattgtg caatcatatt atattaatat atatttacat
    15421 aatagtattt ttctttaatg tagattaaca tatgatatgt tttaaacatc aatccccttg
    15481 ctttcttata tatatttggc aacacatatg agtttaataa attatataca atgttgcttt
    15541 cttcttgtat tttttcaacc aacaagttat ttataaaatt acctttctca ttttcaataa
    15601 tctcactcca atttatctta acttttaata tatatcttcc cactaggtat acacactcta
    15661 caataaataa taaaaataca atgcagtcag tacctgttgt cttatataaa ttacccgata
    15721 ctaatggctt tgcgaagaaa ttataaaaat tcaaagtatc acccgtataa tcaaaaagta
    15781 ttaatagcac tcctagaact ctcaatactt taatattcaa catgttttta tctttattat
    15841 ttttgttttt catatatact acctctcttt tctttagatt tgattgtaat ctaatactaa
    15901 ctcttttatt tgaggtataa atgaatttaa ttggtagtta ttttcatttt tactagaaat
    15961 taattcacat tactaaacaa aacttatatt taaacataaa aatagcctat ctatctttta
    16021 ataaatacaa aaataaataa gctactaata ttcaattaac ttttatcact taaaacatta
    16081 ctatctataa atttaaatat tttattgaag tatactgtac tatcatgttc aaatccatca
    16141 acatgaccaa taccttctat aagccatagg tcttttttat catgtttaac taaatcatac
    16201 attttcttgc tattttgata attacaaaca gtatcttttg ttccatgtat aaacagtatg
    16261 ggtacattag atttctcaac tgactcatat ggttttacat cactgtacca aaatccagat
    16321 ttaaacttat tatataaatc tccacaagtc accacataat tgattaatat atctggtatt
    16381 cgtttttcta gtactcccat tctaattgca tctttcattt cagaataagg agaatccagt
    16441 atataaaaat cgaccttatc atcttttgaa tttatctctg aatgcatagc agctgtccct
    16501 gcccccatgg aaaatccatg cacacctaat ctcccttcag gaaatttatt ttttacaaat
    16561 ttaactagac tatctaaatc atacctttca taaagaccaa atgtgtaatt atctccacca
    16621 ctattaccag tatttctttg attataaact aatacattat aacctttatc taaatatcta
    16681 tatgctacct ttaacatttc atagtaacta cttccaattc catgaacaag tatcatagta
    16741 tctcttgtct ttatatttga agttataaaa agactttcaa gcttgtaatt attagaactt
    16801 tgtatcatta attcagatgt cttgtagttt tttaatttct ccagtggttt atcttttctc
    16861 tcagaaaata tttttactat atcttcgttt tttattaact gctctaaacc aactgaacta
    16921 ttatacacta attttccaaa tatataagat acaattaata ggattaatag aaacacaatt
    16981 gatataatta taaatttatt tcttaaacta atacaatttt taaaactatt tttcatattt
    17041 atttaccata caattttcct atattatatt ttctaagtaa tagcataatt gattatacta
    17101 ttcttataat ttctattata cctacttatt tattaaaaat acaaattata ttttattaat
    17161 ttttactact tgtaaattat tatcttatac aatgaatttg tattatataa taagtatttt
    17221 taggattctg ttacataata ttatttaaat attcttctat atttctcttc ttcttcttca
    17281 gtaaacacat taaatacaac ttttaaattt ttttcaggat tttcttttag ccatagtcta
    17341 actctactta cagcaagatt agctgcctct ttttttggat aaccaaatac acctgtagaa
    17401 attccacaaa aaacaatatt ttttatttca tcaatttctt ttattgtatt taaacatgat
    17461 ttataacaat gaagcagttg cttttcttgc tctttacttg gctgaccacc agatacaata
    17521 ggtccaacag tatgtactac aaattttgca ggtaaacaat aacctctagt tatcttagca
    17581 tctccagtat attctaaatg cccttgtttt tttattattt tgtcacaatc ttctctaagt
    17641 ctaggacctg cgcatgaatg tatctcatta tccacgcata gatgtaatgg ttgtaaacac
    17701 cctaataact tgttatttgc agcatttact attgcatctg ctcttaaatt cgttatatta
    17761 cctctccata tggcaatccc ttcctctact tcttctatat catttacatc tactaactct
    17821 ctttcttcac attcaatcaa caataaatta tgtaacatat taagtatctc tggagcaatt
    17881 tcttttggtg gatatacatt caaaagtcct ctaagtatat ctctaggctt aatttcatca
    17941 tttgataaat tgtatatttt agaaggaact tcttttgaaa aatatgcaat taaagtgttt
    18001 atattttttc ttctttgttc gtcagttaga ggcttgatag ttttatcaaa atcctcaaat
    18061 aaatttacat catcagcata atctctccac ttcatagcca tctctccatt cttgtatttt
    18121 agacttaaaa ataattatgt atttatgtac atatttattt atttctataa acacctacat
    18181 aaatcctgtt aaaagtgtaa attttttatt atacaacttc tctggttatt tctattgatt
    18241 gatagtaaat tattgtacct aaatttaatc caactaacaa gtaactaaac ctactttcct
    18301 aactgcaaaa tacactatgt ttgccacttg tatctcattt gtagataaat aaagtcccta
    18361 caaatacatt aactaggcta tgtactaaac tgtaggaact ttaaaaatat attattctta
    18421 attaagtttt ggccaataat ttttatttgc ttctattaaa tcatctagta attcttttgc
    18481 aactgttgca ctaggaactg tcttagatag cataagtgct tgccacaatt tattataaga
    18541 cttctcaatc caagcatcaa ctactaattt ttcaactgct aattgttgtt ctattaaacc
    18601 tttttggaat gttggtattt tccctattga taatggttca tacccgtctt ttccaactat
    18661 acatggaatt tcaaccatag catcatcttg tatattttct atagctccat tattttgaac
    18721 tatcaataac atacgctcct gtgtattaaa agctagagct gtagctaaat ccactataaa
    18781 ctctgcatgt attcctatct taagcccact gcccttagaa tgtccttgct tttctatcaa
    18841 tgcacatgaa ccaaaaactt ctttttctct tccatcaata acttcattgg cacgagtata
    18901 atttacatca gaatgattta caacataatc tggaaataga tagtacttta agtaactact
    18961 tggtattgta gttaaatctg tttttactat ttctttagta aataaattag tttcaaacca
    19021 actatctttc ttgtcatttt tcattccttc ttcaccattt ataaaaccat attcttttat
    19081 atgttctact aattttggta aaagttcatt tcctttttta tctttaattg aagtatacca
    19141 accaaagtga tttaatccaa agtatcttat atccatatgt ttacgagatt tgagtcctgc
    19201 aatccttgct atattatgtt ccatacctat tggcatatca caaatattta ttaccctaga
    19261 attaggtctc aaccttctaa cagcttccgc tattattgat gctggattag aataatttaa
    19321 catccaacaa tttggagaat acttttccat ataatctatg ttttcaagaa caccttctat
    19381 agacctcatt ccataagcta ttccacctgg tccacaagtt tcttgaccaa ctaccccatg
    19441 ttttagaggt atcttttcat ctagttctct catttcaagt tttccaactc ttatatgagc
    19501 taaacaaaaa tctacatctg tataagcctc tttagggcat gttgttgcta aatactctat
    19561 attagggtcc ttttccttta ttagtatttc acaagcttta gctaccttat tttgtctttc
    19621 ttcatcatta tcatataatt tgattgctct taatggtaac ctatctaagt tatctagcaa
    19681 cattaatatg atttctgggg tatatgtact tcctcctcca gctattacta ctgaaaattt
    19741 ttccataata atatctcctt ttctatttaa aaatttactg tatttataaa taaatttata
    19801 aatactagta ctttaaagta aaagggagtt catttattaa atctattgat ttaataaact
    19861 atagccaaac ttcaagcact atttacaaga aatttaattc ttattaattc ttgggtccat
    19921 catctcaaat atatctacaa gttcagatac taaagaatga gcaaataatg catttgaata
    19981 atggtcttca gagtgcacaa ctaaaaaatc agtcccacta tcttcactat tcatagcatt
    20041 tgatagtaat tctaaatgat gctttgatgc tgtttgtatt ttctctgagg ctgaatttaa
    20101 aaggtgtttt gctttattgt agtcaccttt ttttatttcc ttaagagaca tcattacatc
    20161 acttttagca tctcctgctt ctgatattat actaaatact ttttcttcca tttcaggtac
    20221 catatccttc tccttctaat gacttgttaa aaatcaaagc tatcatcatc taatgttgta
    20281 gctaattctg catttttatt taattcttcc ttctccatta tcttaaagaa tggataatat
    20341 atcaaggtca taagtattat acttgcaaat actaatacga ttgccttcca atctaatgtc
    20401 atcaaaaatg ctgcaacccc tggaggtaaa aagcctggtg gttctgcaat aggtaatcct
    20461 actaatccta cttttaatgc cataaaatta agtgctccaa ccaatacagg tccaaataca
    20521 aatggtacaa acatatatgg gttcataact attggtgttc caaatattac cggctcattt
    20581 attccaaata gtgatggtaa aatcacaact tttcccatac tattaaaact cttacactta
    20641 cttcttgaca taaggtacac taatcccaaa gttattcctg aaccagttac ttgcaagaaa
    20701 ccatagtaaa atcctattgt aaacacatat tttgggtctc ctcctgctgc tatcagtgtt
    20761 gcattttctg ccatatatgt agctgctatt ggcatccata tacttgaagt tattgctgct
    20821 ccatgaagcc caaagaacca taataattga gttataaata gtattatagt tactgcaaaa
    20881 acattatcta cactgcttat agctggtgct aagaaattca ttataatagc tggtagtggt
    20941 tgacctgaga catttttagt tattgctgct attgctgtaa ataacattat acatactatc
    21001 attggagtta aatttctaaa acttttattt actacactag gtacttcttt tggaaatcta
    21061 attacgagat ttttatcatt cataaactta gtaatatcaa ctactaaaag tgctactaat
    21121 atacttacaa ataaaccttg tgcatctaaa taacttgtat ctaatacacc atctactact
    21181 tgtccacata caacaagttg tgcaacaact gctgtcatgc ctatttctac atgattaaga
    21241 ttatattttt tagctagtgc ctgagctata aaaaatgctg aatatataga taataaactc
    21301 atagtaaatt tattagggat aagtaatgta tccatatttt cagataataa tgtttttgca
    21361 cttccttcag gtaacatatt gtttaatgcc ataaataaca tacttaaaga accaacaatt
    21421 attattgggg taatgcttat cataccttct tttatagctt gaagatgcct ttgtttttca
    21481 atcttttgtg ctattggaag aagaaatctt cttaaattat tttgaagtgc ttccattttt
    21541 attcccccca attatctatt tttatataat tcgattgccg acttcataac agaaggagca
    21601 tccatacgac cataatgctt agtatctatt aaaccaactg ctattttgta attcttacaa
    21661 gcttcaacta catcatcttc cttaaaccta atttgtggtg ctacaagtac tacatctgta
    21721 tcattagctt caatgtatga ttttaaattt tcaaatgatg ttgcctctat atttatagtt
    21781 ttagttttta acttttcatg ctcactacaa aaacttttca tcttttccac aagtacacct
    21841 gttgaagctc catttgcaca tactaataaa atattcataa ttaccctcct acaatatcta
    21901 acctctttta ttttctaatt ttttatcagc caattcttac tctaattcct atcagttttt
    21961 actttaattc ttattatttt attttaattc ttattaatat ttactctaat tcttagtact
    22021 gccaatgatt aaacttgaaa gccgtatcta taacttcttc ttgcttatat ttttctaata
    22081 tttttaagta tctttcttta taatcattat ctacttcttt ttctggtatt actatactag
    22141 cttcatttaa aaagtggaat gaatcccttc ccatttctaa tcctctatat gtatgcttgt
    22201 caagtgccac gtccataact tctggaactt tacccattgc aaatcctttt ataattatat
    22261 tttttaataa atcacttgat ctatctttct cacatgcaca aagatatctt attgcatgta
    22321 tgaaaaataa aggtctgtct ccatctgcat atgaatattc ctttcttact tgatttaaat
    22381 tatttatgat tattacagct gatggatccc ccattcctat atcttcaact gatattgtaa
    22441 gtatccttct ccatagctta tcttctaatt ctggtgatga tatatatagt tcataagcaa
    22501 attcacaagc ttcttccacc atatttcttc ttatagcttt ttgaaatgac gatattattt
    22561 cgtctgcact aaatccgttt cttgtagtca ttcttgccca tgggtcattt gcctttttat
    22621 ctcttaatat tttatcttgc atattattta ctccttttta tttaaatttt aattatttat
    22681 attactcatt taataatata agcaagtatt atgccagttt ttttagacac tttaaaacac
    22741 tgttatttca atcattttta tcatcaaaac ttagatactt tgtctaagta tatttatatg
    22801 tgtctaatat taatattatt ttagactaaa aaaaaattac caaataagtt tttatactca
    22861 ttggtaatct ttttcgatta tttctgcata ttctaatatt aataatattt gatagcactc
    22921 tttttcatta atttctatat tatatttttt atctaattga gatactatat cttttatttt
    22981 gcttagactc ttgtgataat caagtgtttt ttctttatca agcttttcaa tttctctagt
    23041 atttccaatc aacccatcta ccatgaaagc taaatgcatc ataagtccaa ttactttatc
    23101 gtatttcttt tctatattaa atactaaaaa caattcatca gacacatatt ttatatcttt
    23161 aaatattcta ttataattag agctttttaa ggatgtcttt aaaccttcaa ctacatcatc
    23221 ataacctata cttatcttta taatattttt taatttattg atattttcat ccttatctat
    23281 atcttttttg ctaatatagt caattcctct aatattaatt ctttgttcat tgattactgc
    23341 aacaatatta ctactttctt taagtttcgt aatatttaaa cttaaatctt tttctgtttt
    23401 atatgggaca gatataatat ctattccatc taagtaccta ctcatctttt cttctaatat
    23461 ttttcttgtt ttattatcta caccttctga aacatgtgat gctataataa ttgttttaga
    23521 cagattttct ttattttgaa tttcattagt tccaaaatag tgctttctcg tgttagtgat
    23581 agagttgtat acgtcattta aaggtaatcc catattggcc ttctgaactg cttctaacac
    23641 agttaatgtc gtcaccatag gaattactct tactccacac tttttttctt tctttataat
    23701 ttcatcaaag gagtttaatg aacccatatc tgtaaatagt aatacatctt tattgtctat
    23761 attattaatt aaatttttaa aatctacaat aacttcatca tagcttttat ctaacttcat
    23821 atcgaaagaa atagcactgc actttccaat aatatcacca actgctttta ccatactact
    23881 tgctgtagtt tctccatgca tagcaactat aatattaatg ttattcacta ttttattttg
    23941 ttcatcttgt ttagcttgta ctatgaataa tgtaatatat gctacagttt ctatagataa
    24001 aaataaatca aattcatttt caattattcc aactattttt aatgctgttt taaattcact
    24061 tctgttattt cttctaattt gattcaaatc tatgttgttt gtagagctac ttttatccaa
    24121 tacgcttttt atataaattg ccattgactt aaatattgat tcacttatgt ctatttcttc
    24181 atcttcttta ataatttgtc ttattgattc acacatgctt ttaaacttaa cattattaaa
    24241 tagagaagca tctgctatat tcatttctac agatttatta agattagtat ttactgcatt
    24301 aataatattt atatcattgt agttaataac ttcttcttct cctaatgacg taaatacaaa
    24361 atattctatt ccatgtgtta taacttcatc tgctttaata gacttctctc tataatcatc
    24421 actcacttca tttatagatt tatcaaaata ttttttctct atataaactg gttctttatt
    24481 atctctttta tatcttagat atccatttgc aacagctaat tttatatcat tttgaagttg
    24541 acctacatta tttttacaat cataaagtaa tagtgattta attatttctt tatgtacttt
    24601 tattgaaata gaaatacttt ttgattcttc cttgaaaaaa tttttgacta attccattct
    24661 ttcaatcatt gtcctatctc tcaatgatgg tatttctatt ttcattggta ttcttctatt
    24721 gaatgtatct aatagtgttg atgtcggatt ttctgtagta gcacatatta ttaatacctt
    24781 agactctctt tctttcgttg catctcctag tcttttaaat gttcccttat ctataaaagt
    24841 aaacaacatt tcttgacctt ctgctggtag tctatgaatt tcatctaaaa atagtatccc
    24901 tccatttgca atttccaaaa gaccttttct ggaagattct gcacctgtat aagctccttg
    24961 ttctacccca aaaatatgtc ccataagtaa ttgtgcatta ttagcataat cagcacaatt
    25021 aaatgttaca aaaggagctt tattttttct aattccacta tctatagaat aatcgtacat
    25081 atatctagca atcatactct ttcctactcc tgtttctcca tagataatag tatgaagtcc
    25141 atttgtagga tagagcattg cgaccttgca tttttctatt ataggtctta aactatgttc
    25201 tttacctatt agtgtttcaa aactatcttt ttttattttg ttttctactt ttgttccttt
    25261 ttttatgcca agcaactcac tttgtgtatc aagcaattca ttcttgttaa ggttaagatt
    25321 tgcagggata taatacacag gtcttccttt tatcttcgta accataccct cattgaataa
    25381 ttggtttaaa tatgtactta tattgtttct tgcaattcct gtcttttctg ataattggtt
    25441 agctgtaatc cctttttcat tttccctata tgatgattct aaaactttca ttactttatc
    25501 ttttttatat atcattacaa gctcccctac attatagtat tttattaact aaaaccccta
    25561 tacacacttt attttttaat aaactcttat tctttaacta aggttataat ttatcaaact
    25621 ttccttactt taaattatag aaaattattg tagaaaaaat aagattattg ctcatttttt
    25681 ctacattact atttttaatt ttatagtact ttcttaacaa catactttac ttgtttttaa
    25741 ttttattata tttgaattta ttatatttga aaagtagaaa taaaaaaccc tattttggag
    25801 tagttttaaa atttctccaa aaatagggtt ttagtttgta tttcaaataa atacctgttc
    25861 aagtattttt atactaaaca acttttaaat tttgttgata tttatttatt tctgatttag
    25921 aagaatatac aaaatgtcca tctgaaacct caatccaact taatgacttt tcatttgaac
    25981 aatcatatcc attagaatta tactctattc tatttcttga cttagcatat ctagggtcag
    26041 gaacaggaat tgcagaaagt agtgattttg tatatggatg aataggatta ttatacaatt
    26101 catttgaatc tgccaattct actaattttc cattatacat aactcctata cggtcactaa
    26161 tatgtctcac catagataaa tcatgagcta taaataaaca agttaaattc ttttcttgtt
    26221 gcaatttttt aaataaattt actatttgtg cttgtataga cacatccaaa gaagctatag
    26281 gctcatcagc tattataaat tctgggtcta ctgaaagagc tcgtgctatg ccaatacgct
    26341 gtctttgtcc acctgaaaaa tcagatggat acttatttgc ataacttcta tcaagtccta
    26401 ctagctttag catctcatat actttattca ttctatctgt tttattttta cagatacctt
    26461 gaatttttag tggctctgaa attatatctg caattgtcat acgtggattt aatgatgaag
    26521 tagaatcttg aaaaataatc tgcatacttt tgtttacatc tttttttata aatttgtacg
    26581 tttttttatc tgatatgcaa tttcctttgt aaattacttc tccaccagta gattcatgta
    26641 gtcttattat agttttacca gttgtagatt ttcctgaacc cgactctcct accaatccaa
    26701 atatctcacc cttatatata tcaaaactta tatcatcaac tgcttttaca gtagtacttt
    26761 tgtccaaatg aaaatattgc tttaagtttt ttatttccag tattttttct ctattcatta
    26821 gaaatcaccc ttccacaatt tactctaact ggtacatcta cttcaggtgc atctgggtgc
    26881 aataaccaag ttgctgcact atgtgtatca ttaattttaa acattggtgg ctctttttca
    26941 taatctatct taagtgcatt tttattcctt attgcaaaag catctccttt tggtgggttc
    27001 aataaatttg gaggcattcc aggtatatta tataaatagt catcctgcga atctaaagta
    27061 ggtaatgaac ccaacaaccc ccaagtatat ggatgtctag ggtcataaaa tatatcctct
    27121 acagttccta tttctacaat ctttcctgca tacataactg ctattctatc agctatagtt
    27181 gcaactactc ctaaatcatg agttataaat attattgaca aacctatttt atgttgtaaa
    27241 tcctttatta aatcaattat ttgagcttgt atagttacat ctaaagcagt tgtaggttca
    27301 tcagcaatca atacatctgg attacaagca agtgctatag ctattactat acgttgtctc
    27361 attccacctg aaaaatgatg tggaaattgt ttaaatcttt tctcaggatt atcaattcca
    27421 accaattcaa taagctctat ggctctcttt tttgcctcac ttttacttat accttgatga
    27481 atgattacag cttctgcaat ttgctttcct attgatatag ttggattgag agatgtcata
    27541 gggtcttgaa atatcattga tatattcttt cctcttatat cttccatctc tttttcagac
    27601 ttcttaacca aatcacttga tttaagtaaa acctctccat ttttaatata tccattgtat
    27661 gggagtatcc tcatcaaact cttacacagt acagatttcc cacaaccaga ttctccaact
    27721 atagccaatg tctcaccttt ttttagatta aaagatacat ttcttacagc ctgaacttct
    27781 ccttcttcta ctttaaaact tacagataga ttattaacct ccaataaatg ttccaaaaac
    27841 catcaacccc tatgctattc aaaccttagg gatatatttt tatccctaag gtttctaatt
    27901 aatttcagta ttattcaatt gtccagtcag ctatgttcca gaatacacct acaccatgat
    27961 gacctaaaac tgtatcaggt gttaatcctt ttatgtttgg ttttccaaca tatattgcat
    28021 ctatataagc aataaatgta taaggcatat ccttagtcat ttctacttgg aattgtttat
    28081 ataactttaa tttttcatct ttatcttctg tttctcttgc tttttgaagt attttatcta
    28141 tagtaggatt agagtaagca ctgtaatttg cacctttatc tgttccaaat actttatatg
    28201 tatggtcatc tgggtcaaat ggacttcccc aacctattaa atgagcatct tgattagccc
    28261 aatctgtttc agttacaaca acagctttag catcaacacc aatttctttt aactgttgtg
    28321 cacatatttt agccatatca actctaactt ggtcactctc acctgctgtt atctcgaaag
    28381 ctaacttagt accttctttt tcatagattc catcagaacc taatttccaa cctagctttt
    28441 caatttcttg tttagctttt tctggattat attcaaactt ttctatatca ggattattat
    28501 atggtcccat ttgtaaaggt gaatatgctg gtacaccatg tcctaataat acactgtcaa
    28561 ctattgcttt tctatctatt gcatagctta aagcatttgg taatccttta gcttttttat
    28621 ctttaaagaa tttagaatta aaattgtata atatacctct atagtcggct gttttcatta
    28681 tatttacttt aaaattcttt tcgtcttttt caaagttaga catatcttta ggagttattt
    28741 gagctaggtc taattctcct gattttaatt gcattgcctt agccttatca tctggaacaa
    28801 tcttaaagac tactttatct aatcctggtt cttttacaaa ataatcacta ttcttaacaa
    28861 gtgttatact ttgacctttg tcccattttt caagtttaaa tggtccagta cctattggtt
    28921 tttgattaaa ttcatcagta gctatatcct taccctccaa agcatgtttt ggtaatactc
    28981 ctactgttaa ataatcaagc attgctgtat ttggagcttt taaagtaatt tttatagtat
    29041 tatcattaac cacatcaatt tttgttatat cttcataatt tgatgctatc tcagaagcat
    29101 tatctggatt cattatagtt tctattgtaa acttaacatc atttgctgta aatttctctc
    29161 catcatgcca cttaacatca tctcttaaat taaaagtata tgtatttgtt gcctcatcaa
    29221 atttccagtc ctttgctaga caaggaacca ccttattatt ttcatcatga gctgttaatc
    29281 cattaaatat taacgaattt atttctccat gttcatatag tgcaggattt atacttgtat
    29341 agtcattact tccataaaca agcacctttc catctttagg cgtatctgcc ttcttatctt
    29401 tgtctcctcc actagaacaa cctgccatta gactaagtat cattactaaa cttaaaactt
    29461 ttaacttctt taatttcatg atgctccccc ttttgctttt tataaaatat ttttcaaagc
    29521 tttgacccca tttatttaaa tagatatttc aaatacctca gtatttgctt atttaatact
    29581 caaatgctga tttataaatt actagatttt ttattattat tctttcttat ataattacct
    29641 atatttgtta tacacaccaa tgtggtaacc aaaaatatac ccggaatcag tataatccac
    29701 catctatttg aaagtaatgc ttcctctgac aaagataaca tacttcccca agaaacaatt
    29761 tctattggta aaccaattcc taaaaagcta agtgtcgatt cagtaccaat agctgcgcct
    29821 atgtttgtta caaccatgaa cattattgat gccacaaaat ttggaagtaa atgttgcttt
    29881 aatacataga agaatcctcc tcccatgctc ttagctgcta gtatgtattc gctatttttt
    29941 atttgtctaa cttctgttct cactatctta gatatattca tccaacttgt tatccctatt
    30001 actatggaca tagatactgg atttgaattt ccaagtatag cttgtacaaa tattataatt
    30061 aatattgaag gtatactaag tatgatttct gtaaacctca tcattgcatc atctacgagt
    30121 tcagatgccg aaccacttat actaccataa acaattccta taacagttga tattacagtg
    30181 gaaaatagtc ctataaataa agatattttt cctccatacc atatcataga atatatatct
    30241 ctacccattg agtctgttcc aaataaaaaa gttttatttg gagctaaatt agaacttgct
    30301 aaatccatgt atgttggttc atgtgtcatt ataaaagatg agaatacact tccaatgact
    30361 atgattgata aaataataat tgatatatat ggtaattctt taattttttt agataaggtt
    30421 ttctttgttt ctatatgttc atcttctgct actaaaatat aattttctcc tacaatttca
    30481 aaatcactat attgtggctt cattagtatc acctctatca tatttcattc tagggtctat
    30541 tttattgttt ataatttgag ctaacatatt cccaaatact actaaagccc ctgttattaa
    30601 gcataaaact aataacatat tataatcatg atactttgca ctttcaaaac aaagtgttcc
    30661 taacccagga taagaaaaaa ctttttcaac tacataagtt cctcctagta tatgaggtat
    30721 cgaaatggcc attatactaa tatatgatgg cattatattt ctcaggcaat gcttaaaaac
    30781 aatagttcta ttatttaaac cttttgcttt acataaaagc acataatctt ctcttatttc
    30841 ctctagtaat ttatttctta tcatatatgt gtaataccat agatggctta atataagtac
    30901 tgtaagaggt aagattagat gtgatatcct acttaatata ctggactctt ctcccattga
    30961 ataagcacca ctacttggaa gtatacttaa gtttatacta aatataagta tgagtactaa
    31021 ggcaacccaa aatgatggaa tacagttggt tattgttcct aatttgcata tgattctatc
    31081 aatgagctta tcttcatgta atgtacaaaa tattcctaac aataaggcta gcacaaaggt
    31141 aagtatatat cctgaaccac ctaatattat agtgtttata tagacatcat ttataacaga
    31201 agtaacattt tgtttatatt taaatgaaat tccaaattcc ccttttgatg catttgaaat
    31261 ccattttata tattgactgt atattggttc atccaaacca agttttttca tagcattttc
    31321 tttttcttgt gtactcattc tttctactcc atccccatag taagagatta atgggtctcc
    31381 tggagcaagt cttgccatgt aaaaaacaat aaaagataat aaaaacatta ctaaaataaa
    31441 ctgcaacaac tttttcagta tatcctttaa caaaccccaa cccccctttg ttgcaataaa
    31501 aaaaaccaca taaaagcaaa cgtaaagtaa cgttaaaacc ttcgtggttc cattatcatt
    31561 aaacaaaatt aaattttcta tatttatcta tgagttaatt ttagcatcat ttattaactg
    31621 tgtaaacaaa ctttttaaaa attgttaaaa tttcatcaaa ttattttatt tatacctaaa
    31681 ataaaatttt tatattctat tcttcattat tttctataaa attataaaat tttaggttga
    31741 ctatataaga aaagtatatt atattagttt gtatgcaaac catttaagag gtgattttat
    31801 tgaaaagtaa ttatgactgt tttagaatag ccatgctctt aaaagaactt tattctaaaa
    31861 ctatgtacac agtagaagaa aattttaaag aaaatggatt aacacaccaa cagattatag
    31921 ttataaaact gatagctcat aatcaagaac ttacaatatc acaactatgt gatgaaatgt
    31981 ctttagctaa aggtacggtt tcaggcataa tcagcagatt agaacagatt ggatatatag
    32041 aaaaattcaa gaaatctaat gataaaagaa atacatacgt taagtttaca acaacaggat
    32101 ttgaatttgc cacaaatttt aaaattaaaa tgcaagaaag ttttgatgat atttttaaaa
    32161 attgtgatga aaacgaattg agtgacttag taaaaaatct tagaaatata ttggcaaaag
    32221 taaaggagag ataattatga attataaatt aatacttgca atagtattta taacatcagc
    32281 tttaattttc tacactatag gtgtatttgg agaaagaaag gcaaaaattt taaaaaagaa
    32341 acatgtaatt atattttggc ttggttttat atttgatact ttaggaacat ttacaatgag
    32401 taacatagcc aacagccata cttttgaagt aaaatctgct ttatcacaaa accttcatag
    32461 tattacaggt cttcttgcaa ttgtgcttat gctttttcat gcaagttggg ctacttttgt
    32521 cctatacaaa gatgatgagg aaaagaaaaa attctttcat aagtttagta tagttgtatg
    32581 gactatatgg ttagttccat attttatagg tatgtttatt ggtatggctg gatagtaaaa
    32641 taaaaagtgt gtaagaaata ctgtaggtaa atttgtataa gacaaattta tctacatcct
    32701 tctttcacac ttttacatat ttatttataa tactccatta tttctttcat gtttcttaat
    32761 acctatatag aatatttaat tatttctata gtattttatt attttctcac actttttcat
    32821 atatatattg ttttactatt tcttcatatg tatatatgaa atattccatt atttctataa
    32881 atgttttatt atctttctca tactttttca tatatatatt gcttcactat tttttcttat
    32941 ttcttcatat acatatgaaa tatttcatta tttctataat gttttattat ctttctcata
    33001 ctttttcata tatattgctt cactattttt tttacttctt tattaaatac ttatgtataa
    33061 catacattta ataaacgcta actattaaac acctaatact tgagttactc ctggaacttc
    33121 actcaccaaa acattttcaa taatagcttt gattgtcata gtcgctcctg gacacccact
    33181 acatgcccct tgtagtctaa ctaaaaccac accattttca tttacatcta taagctctac
    33241 atctccacca tctctttgta atactggttt tatcttttcc tcaagcactt tttcaacttt
    33301 ttctctcata gctattcccc ccagaaatat tttattagca aaattatacc aatggtttaa
    33361 atttttgtcc atctaatgat ttatcaaatt caatttttgc ttcatcaata attttagtat
    33421 ccataaatac atcataagca ctacaagcca gtactttagc tgaatttatc atagcagaaa
    33481 atcctatatt tgaacctgct gatgaacatg actgccatgt atgacttgca attccaacag
    33541 gccaagctgc cattgcaaat tgagccactg gtattacata acttacatct ccaacatcag
    33601 tagaaccagc tagtccttta tctttgtcac cgtcaactat ttccttgtgt atatagcttt
    33661 ctacaataga tttatcattt tgtaatgatg ttgctacacc taatctttct tctctagtaa
    33721 gagtatcaca caactttttt gcaaattctt cttcttcagg agtattttta gggcaaccaa
    33781 caaaaaccat attgtcataa actaaatcag tcaaacattg gtttggtata tagtcatata
    33841 ttccatctgt aaccttatac tccatttcag tttctgtcat cattgctgct ccttgagcta
    33901 cttttattaa tctatcaaga acgtgttctg catcctttgc ttttttacct ctaataaagt
    33961 accaactttc agcaaatcca ggaactacat taggtcttcc accaccatta gttataacat
    34021 agtgcattct tatagagtca ataacatgtt ctctcaaata attagctcca acattcataa
    34081 tttcaacagc atcaagtgcg cttctaccat tatgaggagc ttgagctgca tgggctgtta
    34141 tccctttaaa tttaaatttt acagataagt ttgccagact tccacctctc caaggtgcat
    34201 ttatatcaaa aggatgccat gtaaatgcac aatctacatc atcaaaacag ccatttatac
    34261 acatagctgt ctttcctcca ccttcttctt cagcaggaca accaaaatat tttattgttc
    34321 cattaaattt attttcttct attaattttt ttatagaaac aactgctcct acacctgcta
    34381 cacccaacag gttgtgccca catccatgtc cagcttttcc ttctaccaat ggctttcttt
    34441 ctgcacttac acattgtgat agctctggta aagcatcata ttctccaagt atgccaatca
    34501 caggtttacc ctctccaaaa cttgctataa atccagtagg taaattttct acttcttcaa
    34561 ttttaaatcc ttttgacttt aggtattctt tttgaatgct acttgcatac ttttcttcaa
    34621 aggctaattc tggattttcc catattttct tagataaatc aattaattct tctcttattt
    34681 cttcaacatt atttatgata tactctttca taattttcct ccaaatatat gtatttttga
    34741 tatgctttac agattatatt atatactaac attctattta ttcacaattt tttgttaatt
    34801 aattttattc atatgctctt ctatggagta tatcttatct tcactttgcc tcttcataaa
    34861 tttaatgtac tctatcttcc ataaagtagt ttcagttttc tcatttaaca taaattctaa
    34921 tatattaatt tctctcattc taatatcttc ataaaatact acactaccca atattgccaa
    34981 ttctaattca cttaatgatg caaacaacat taaatactct tctccaaata aacattcaga
    35041 ttcaagaata tcgataaagt cttttttagc attgatattt tcctttatta ttcttatttt
    35101 ttcaattata gatgtactat ctaatatctc attgaatatc tctctaaacg attcatcatc
    35161 caatttactt tcatcatcaa caatataatc ttctaagtgt tcctttttat ttttactcac
    35221 agttaccata cttttaaaag tgttactttt tattgagctt agcacagatt caataaatat
    35281 cttttcatat tttttaatat atcttattac atcttcatct tctatgctta atctttttat
    35341 taatacttca attgcttcca taagtagtaa agttattttt ttatttattc taatttctct
    35401 gttaatttct tcaaaatcat tataatccaa attactatct acttcagtgt taaaactatc
    35461 atctacttca atcaattcaa ttatattact ttcttcagag tctataactt tatcttcagt
    35521 aatattattg tacatatttt taaattgttt ttctaaaaat tcatactcat attttgaaat
    35581 ttctaaatta gtcacttcat ttcctaaaat agtagaaaaa atacaattat ttataataag
    35641 ttcaaataaa tttacaagta tagcatcaca atctatttta taagtcaccc catagtcata
    35701 aaataatttt tctctatctt ttttcttaaa taggttacaa aatctatttt ctatatctat
    35761 actctcaata taattcttca cataataaat acccttaaca tccatatcat ctagtgccaa
    35821 tggataatct atacttactg gaacgtcctg agcttcaaat tttacaatat aacttttaaa
    35881 gaaacttgat aactccatca aggtatcatt atatgctatt gaattagtat ccaattttct
    35941 actcatcatt agtttataaa aatcttttag cctaactaac tcttcctcta atatttcaat
    36001 gcctccaagg tacacttttt taacattctt agcagtcaac tccacaactc ttttttctat
    36061 atccccaagc ttatttatat atgcatcaat agcataccat attgaaatca acagtttttc
    36121 ggcaacttct gtttttactg atgtgctttg accatttgta tattttttaa tcaaatccat
    36181 aagtattaga gatatttctt gttgtatatt atatacttct ctttcatcta tcactttaca
    36241 atttagacat tccttaagta gagatatgga atattggttc tttattaaat tattttcgtt
    36301 tatattaata ttacttaaat tatttaacat acaaaatctc tcctatcata tattaaactt
    36361 ctccaaattt aaatttcttt gcaaattcgt ctgcttctcc tcttacattg tccatattgc
    36421 ctgaaaattt ttcataaaat tcacccagta tttctataat ctcatcatca catatcttgt
    36481 catcagtttc attttttaaa taataaaaaa tttcttgcat atcattaata gcctctaaat
    36541 aattgtccac atttgtatat tgtgatgtat atatattttc tattaactgt tttgtaactt
    36601 ttatatctaa ttctattctt ccatacccct ttaaagtatt atctctcgaa tttattatat
    36661 cttttacatc agattcagaa agagctagac cataatctcc agattctata ttagtttcaa
    36721 gcagttcatt tctttctatt tcctttaaat tctgatttat agaactcaaa ataataggtg
    36781 atatcatttt taaattctcc ttttttattt tttccattga ctttttttgt tttttatgct
    36841 atacttaaat taatttatat tattttacca accacttata atagtagtgt taaaagtggt
    36901 ttttgtcaac tcaaaattac tatctcaatt aaacttttca attttctcaa caaaaaaata
    36961 ccatcattta gatatataac tttctataac aacttttaat tcagtagtta tttactaaaa
    37021 tctaaacaat gatatattta ttaattttat ttatcttttt tggctctact tctacttttc
    37081 tttacatttg agcaatttct acaagaaggt gaacagtatt gagatttaga atttgtagga
    37141 atataaattt taccacagta cttgcattta tttaaagaag aaggttcact tgtaattgca
    37201 aaggcataga tagtctcaat agctgttttc aaagaatcaa actcccatgc aataattggt
    37261 ttatctaatt ggcttataga aaatcctatc ttttgaggat taaaccctcc agacataata
    37321 gtaacacttt cagttaatag attagatgta tttttatata cacaaatttg attaaaatgc
    37381 ttacacatat ctttagcaaa acttacaatc caatctagtt gttcagtgta gaactttgaa
    37441 aatactaaat ctaaaatcaa tggtctcttt ccaaaaaact ttggagattc ttctctcttt
    37501 gatacatcta taccccttct atacttttta aaatacacat cttcatcact tgcaaatggt
    37561 ataaacatgt ttacatattc atctgtactc aatatattct cttttgtaat ataattcttt
    37621 tcaatcatca gtactctttc ttctccaact atatctctat tatatacact tgaatatatt
    37681 aaacccaata atccatactt ttttacaaat tcaagtactt ctttctttag tttcatttct
    37741 tttgtctctt ttgatatatc ttgtaatgcc tcttgtccta tgcgaattat atctactatt
    37801 atatcatctg ctacatcaaa tgggttatac atggtaaaca cagcatcttc tgttggaact
    37861 atatataatt catcttcttc agtaatttta tattcataat cactatacct aatccaatca
    37921 cttgcaaaat tccctattaa ttctattttt ttattcataa taatcattca cacctttcaa
    37981 tgtaatatat aatttcatat tactattaca cttttagttt attccatatt ttctacactg
    38041 tcaactattc tttttatatt tttagcatat taaaatttat aatagccttt ataatggatt
    38101 tctagagcta aatttcttgt ttattgatga ctaattcata attaaattta taaaaactat
    38161 ttacatataa aattaatctc tttataattg atatgtaata tatttttaca cttacatatt
    38221 acatatcaat tataaagaga ggtatctaaa atcatggata gaaaacgtga attaaaacaa
    38281 ttatacaaag aaatgaaatt tgacacaggt gtattcataa taaaaaatga tattactaaa
    38341 aagatttacc ttggaaaaag taatgacata aaaagcaaat ttaactctct caaatttcag
    38401 cttggtgctg gctcatgtat gataaaagaa ttaaataatg aatggaaaaa atatggtgaa
    38461 aaagcattta catttgaagt tttagaactt ataaaacatg atgataacaa aacagagaaa
    38521 gattatttag aagaactaga tattttagag atggtttggt tagaaaaatt aaaggaagat
    38581 aatacttatg aaatttattc catataaaaa atcatataat ataagaaaag gagatagtat
    38641 atcaaaatag acatttagtt cataagatac ttttttatat aaaataaagt atcttttcat
    38701 aattttaaca atgaaaaaga ggcttatctc catttgatag cctcttccca tatattttat
    38761 accattataa aatgaacaat ctactaattt tcaatgtgta tattcatttt actaataaca
    38821 ctccattact tctttacagc atccaaacat tgctctataa tactcttatc acttatacct
    38881 attgctgtta ctttactagc tccacttaat atactcttat cacttttatt tgaaactaaa
    38941 acaacaggag cattttttgc aagtggtgat gcaactaaag cataaactaa atcgtctcca
    39001 cttgttatat agaactcttt tgctccatta tagaactttt caataacatt tttattagtt
    39061 tgatatctat caacaccacc aattctagtt gctctatatc tgctcactat gtaatcactt
    39121 aatgtagact ctcctccaat agcatatact ggaacaggag aaactccaaa ccatgggtct
    39181 aagtctgtgt cttcttctgc tgaaggtata tcttttgtca atattatagg agctccatct
    39241 ctataagcaa cagatgcaac acttactgca tcagcttcat ccttaaatcc atttactaaa
    39301 aacatcttat cactgttttt aattttttct atttcacctg caattttata acttgtatct
    39361 actctatcta caccatcaat tcttataact tgcattcttc tcataagtaa cattttttct
    39421 acatctttac ttatagaatt ttctccacct attacatata tcttagtaac tctattcagt
    39481 ctttgaagcg ttgcttctgg tatatcatcc ttattagtta ataaaatagc tgcattattt
    39541 actcctgcaa gaccacttgc agcaatccca tctaccattg tagaatctgc attgataaga
    39601 actgctgttg taaaatcttg cttatctgaa ataagcccag ctgtttcata tttatctact
    39661 ccttgaatat tttcaatctt atctagtgcg tttattgatg ttggaaaact aacacttaac
    39721 agtaatgctg aaatacctaa cgccaatacc tttttactaa tcctcataaa ttacctccct
    39781 aaaacaaata tatttagtat tagtataata taaaatcatt taaaaatagt gtaaaaatag
    39841 ttacactttt ttataattta aataatttta gattaaacac aattcatcca ttaaaataac
    39901 ttcatttatt ctatatgcta agccatccca cgtttttttt gacatatcta cagatgtttt
    39961 cttatttgag ttattcaata aagattgtat cttagaatct attgcattat acaaattatt
    40021 ttcaaaatca tataactctt catcttttgg aataccttct ttctccataa aaggtagaga
    40081 aacatattct atctttccag aattattaat ctcactacca atccactctt taacaccaag
    40141 aatatcagta gtgattacat ctgtgccaca agataaagct tctaaaacta caactggtaa
    40201 tccctcataa aatgatggta acacaaatat ttgtgacctg ttgaatagct cagctaaatc
    40261 tctctgttct aactttccta ggaaattcat tttaaatgga gacttacttg ccagtttaac
    40321 tatctcatta tatgattcga tatcgcttcc agtacctgca aaattgactt ctattaaatc
    40381 tttactatat tttaatttag atatagcttt tataaatgga atcagtccct ttgatttaca
    40441 tatcttacct gcaaatacta tttttatttt atcatccttt attttataat ttttattata
    40501 aaatatatca tcattataac cactaccaat tacaactact ttactttgac ttactccaaa
    40561 agtctttata atatcatatt tctgattttc atgaagagca aatattaaat ctaatttcct
    40621 tatatttgct attatgtatt ctttctctaa atcaatggta tttaactgtc ttaaacaagt
    40681 tccatgacaa atagacataa cctttatatc ttttaccatt tccctaacaa atgcagttaa
    40741 aaggtacaaa tgatgacata ttataagttc tggtttaaaa tcattcatag ccttatctat
    40801 atttacttta aattgatatt tcaatctatt tatcatgtct atatttaaat ctttatatct
    40861 tgtactttca tatggcatag aatcactcat acctacaaca ggaaaattta actccccaca
    40921 attatacttt actggataaa aacttacttc acttggaaaa caatttacat catcatttac
    40981 atctatacct gcaattacag cttgcttatg accgattttt tcgaatcctt ttatcatacc
    41041 agacaaatat ataccactac ctgtactatt tggtttttgt gttattatat gaagaatttt
    41101 cacttttcac ttgcctcctt tctaaaatta aatagtttta tacagtctaa tcattatata
    41161 tatctaaata taaacaagat gaatcatctt ttactttaaa tcttggcact ttaaccgctt
    41221 tatagtcatt atcttcaaaa tcccttactt tattatgtat atatcctata ccaaattttt
    41281 ctgctatacg tatcaattct tcttctttta ttagattata tctatcacat gcacttgtaa
    41341 aaccatcact cgttagcatc aattgaaaat ctttttttat ttcaatgtat ccatatatag
    41401 aattttccac agcttcctca tcaaaatcca atatccaata gccctctttt gtatttttct
    41461 tcaatctatt gcttactatt gtatccataa cactagttct aatctcatca aatgccatct
    41521 cttccaagtt agaaagttta ctcatttcat caaatacagt attatcaaat ttacaaatac
    41581 tcctatcttt tataactttg gaatctccat ttttaataaa caaagcacaa tctcctaata
    41641 aaaaatactc tactttattt tcatgaaatt ttacaacaac tatagaacta gatggtaaat
    41701 ctaatttttc aaccttaaac ccatttaatc ttagttcata ctcttttttt acttctatta
    41761 ttccagtatt aataatactc tttaatgatt cttctttatt tatattttta tataaatacc
    41821 tattccacca ctgaacatac caccttgcgt cactttcagt tgacactaaa ttttttccat
    41881 taagtcctgt agcaccatct aaaacccatg ccccaaatgg atttataccg actacatctt
    41941 cattaaattt agctgttcct tgattacata ataggctata gtttcttata tttaaagcca
    42001 tatcaacctc caaattgaat atctaaaaat aaagttctat ttaaaattat acagcaaata
    42061 aaaaaatcta caacaaaact tgttgtagat ttttacttaa tctatattat ctttatactt
    42121 tcagtatttt aatgatggaa taatctattc ttagtaaatg ccatgactat gttatactta
    42181 ttacaagtta atataacatt atcatcacga atagaaccac ctggttgtgc tataaaactt
    42241 accccacttc tttttgcacg ttctatatta tctccaaatg ggaaaaatgc atctgaacca
    42301 agtgctactc ctgttaaagt tttaagccat gctctttttc cttctccagt tagaggctct
    42361 ggtttttctt caaaaaagtt ttgccatatg ccatcagcaa gcacatccat ataatcatca
    42421 gaaagatata catcaatagt gttatctcta tctggacgac ctatatcctt tttaaatttt
    42481 aagtttaata ctttaggatg ttgacgaaga taccaagtat cagctttgtt acctgcaagt
    42541 ctagtacaat gaatacgtga ttgttgacct gcacctaccc ctattacttg tccatcttta
    42601 gcataacaaa cagaatttga ttgtgtatat tttaatgtaa taagagctat aattaaatca
    42661 cgttttgcac tatctgtaaa aattttatta tcagttggaa tatcttttaa taggtcttca
    42721 tttattaata tttcatttct accttgttca aaagtaattc cataaacatc ctttgtttca
    42781 ataggttcag gagtataatt ttcatctatt tttacaatat tatagttgcc tttacgcttt
    42841 ccttttaata tttcaagggc ttcatctgta tatcctggag ctattatacc atcagaaacc
    42901 tctcttgcta taatagttgc agtctcctta tcacatacat ctgaaagtgc tataaaatcg
    42961 ccataagaag acattctatc tgctcctctt gccattgcat aagcacaagc taatggagaa
    43021 agctctaaat catcaacaaa atatatttgc tttaaagtat ctgataaagg tacccctact
    43081 gctgctcctg ctggacttac atgtttaaat gaagttgctg ctggaagacc tgtagcttct
    43141 tttaattcct tcacaagttg ccaactatta aatgcatcta aaaaatttat gtatccaggt
    43201 tttccattta atacctctat aggaagttct ccattcttca tataaatctt tgatggtttt
    43261 tgatttggat tacagccata ctttaattct agttctcttg ccatatactc atccctctct
    43321 ataataaatt aatcataagt ttgattaatc ctatattaat taaattattc gtactaataa
    43381 aattatatgg catatttatc cattaatcaa ctatattttt taaaactcct attccttcga
    43441 ctttacattc aactatatcg ccatgcttta aatatacttg aggattcatc cccatagcaa
    43501 cccctgctgg agttccagtt gaaattatat ctcctggctt tagagtcatt cctcttgata
    43561 aatcatttat tatagtaggt atatcaaata taaagtactc tgtattagaa tcttgtctta
    43621 cttctccatt tactacacta cttatatcta attttaaagg atgttcaaat tcttctttat
    43681 gtactataca aggtcccata gatgtatgtg tgtctaagct ttttcctcta aaccattgaa
    43741 catgtttatc ttgaagtgct ctttcactta tatcatttac tattgtatat ccaaatatat
    43801 attcataagc atcttctttt gatatattta taccttcttt acctatgatt actgcaagtt
    43861 ctgattcata gtccaacttc tctaccaaat ctccatgtaa gcttatctta tcatccactc
    43921 caattatttt gtctgctctc ttagaaaaat aaattggata atctggcata actacgtttt
    43981 ttataactcc atctgggatt tcattgacat ggtctttata gtttagacct aaacatataa
    44041 tatctttttt aggtcttaca attggggaac ataatttaac ttcttctaac ttataacact
    44101 ttgcgttttc aaatgaattg ttttttattt tttctaaatc tcctgtagat acgttttcaa
    44161 taaaatcatt catatcagta tataatttac ttaatcctaa actattaatt tcatatatac
    44221 tatttgtttc tgaattaaat acacctattt tttcttcatt gctactacaa aaagtcacaa
    44281 atttcatttt tattcctcca ttcaataagt tgattaatat tatttaattt ttattagttt
    44341 atataaattg taattcataa tatttatatg tcaatacata tttatatatt ttaaaaatat
    44401 tcaaataatt tttattaaat ttattagtta ttaatatata agtgctttat aaaacacaaa
    44461 taaaataagg ttgatttaaa attaaattta aaacaacctt ataaaccgat tatactatct
    44521 acctaataaa tatagcaaat aagctcctca ataatataca aaaccctaca tctctaaacc
    44581 tatacttaac taatccaagt acaataccaa aaacagtacc tatcataatt tttgaaataa
    44641 taacttcaaa ggtgtagctt gaattgttat acaatattac ataatttctt ataacatcta
    44701 tgtatcctat attataaatt ccagatagta tagatgttat acagataatt ttactctttg
    44761 actttataaa attacttaga tagttccata tatattctct aaaaaataac tcttcaaata
    44821 ttgggactat caatacagac aaagtcatca tagttacatt aaaaagtaca tatcccttag
    44881 tacaataagt tacaattatt gctaaagata aaaccacaac aaaaagtatc agcttacact
    44941 tttcaataag tatattgttt ttattttctt caaaagcttc tgtgattttt ctatttctaa
    45001 ccttgttata caaagaatta tcttttaaaa ataaaattaa cgataaacca actaatgtaa
    45061 aagaaattaa gtttataata ttaaaatttt caagagtaaa ttcaaattga cttagaagta
    45121 cataattcaa aatcatcctt aatatttgaa ctataaacat caataaaata gaatctatta
    45181 tgcatttttt gtaactatac ttcatcccaa tttaaatcac cccatgtata tgctaatcgt
    45241 aaagtccatt taatacccta cttacagttg ctttgctcca acttgtttga tttgaaattt
    45301 ctttatagct taagccatct cttcttaaac tctttatatt atttatatca tcatcagtaa
    45361 tttgtttttc ttttggctta aggctatttt tatagcttct tagtctatcc aattctgatt
    45421 tcaaactatc tacctcaaac tgtttttttt gaagtttact ctcataagaa tctctcattt
    45481 cattaaactc tttaatgtag tttactttcc agtcctcatc catttttgtt ttaaatatac
    45541 ccatacttta acctccttaa attttgttac tcaagttatg tatcgattat aaacattaat
    45601 ttctatattt atctttaaaa tccttgtaca taacccctag ataagttagt aaaattcaga
    45661 tatattttta caattttaag tatatattaa caatagaaaa aaatataatg tcttaataca
    45721 acatattata tactaaaagt tgttaaaatg aaccttttta tagtctaaat cagagtcacc
    45781 gtatgctttc ttttcttcat caggatatcc tatagaaatt atacattcta cactgtactt
    45841 gctaggtact tttaattctt ctctaatgta cttatcagct tctatattat catcatgcat
    45901 tctatttctt acttgtatcc aacaagaacc taaacctagt tcatgagctt gaagttgtat
    45961 gtatgaagca gctatacttg catcctctat ccaaacatca tttttttctt tatctgcaat
    46021 aattacaata gctaaaggtg cattttttaa gaaaaatcca ccacctttag ttctacattg
    46081 agataaatta attagctttt ctttatcatc taccactata aattcccatg ttctaagccc
    46141 ctttgatgat ggagctaaaa gagcagcttt taatatttta tctaattttt ctttttctac
    46201 ttcaacatcc ttgtattttc taatacttct tcttttcttt agaaatccaa tcatataaat
    46261 catccctttc ataatagcat acactaggtt atattatgcc cttactaatg ctgaaaatct
    46321 attatttatc ccttgtatta taaatgctct ttataatatt ttatcataac agtagaaaga
    46381 tttctccttt cctaaaaaac tcatttctcc gatttttata aaaccacact ttgtaagtaa
    46441 taaattcatt tttgagttta ttgaatatgt atcgctcttt aagtatgata cattgttttc
    46501 tacagctaat ttttcagcaa aatcaactaa cttagatgct attcctgtct ttctaaagtt
    46561 tggatttaca gccattctat gtattaccat tgctttttta ttagatgacc aatttaagcc
    46621 taaatattct tctggctctt cataattaag acatataaac cctgctactt ctccatccac
    46681 ttcataaata tataaatctt gtttcttaat atctgaaaca aagtcttttt ctaatggata
    46741 gttttcatcc cattgtgtat tattataagt cttcatttct tccactgtag attttataat
    46801 ttgcataatg ctttctaaat cattcatatt cgcttttcta atcataaaaa tatctcctta
    46861 agttaatgta tttatttact atctctttac tttaaaccat tcattttcta cgtttttgct
    46921 cattttttct aataacttag ttaccacttg tttttcttca tctgtaaaat tatcaaaaca
    46981 tatctctata cttctattct cttcttcaat tatcaactca taaacttcca aagatttatc
    47041 agttggagtt aaattatatc ctcttttatc aaacttatcc tgctttttat ctacatatcc
    47101 tgcctttatt aatttttgta tagccttggt tgttgtagcc ttgtctacct ttaacatatt
    47161 tgataattcc acaagattta taccaggatt ttcacagatt cttgtcaaaa atgtaaattg
    47221 tcctttttgt aactttaatt ccttgtactt aatatcattt attgaattta ctgctctaga
    47281 taaagtacct acttctctta atatattact atctaaagtc ttaatcaaac acacctccac
    47341 tgaaactact atggctaaag tcaaatagtt tctgttttat aaaatttcac ataatcaata
    47401 tattcatttt tagttgaaag ttcaactttt ttaaatttta ccatattttg gttgaaagtt
    47461 caattaaaaa taataaattt atttaatctt tctttgcata ttaatcaatt aattttaaaa
    47521 ttaaaatgga tatcccaaaa ttaacttttt cgtttatgag gaactctctt ttgtataaaa
    47581 tcaaatatat tttatcattc caataaaaaa ggcttatctc tattttgaga aagcctcttt
    47641 ccttcaatct atattttact ttttccctaa ataaaatcta tacaatatac ttacacacct
    47701 catccgcaaa ctctgtagta gaagctgagc cacccaaatc tacagtcaat ttacttcctt
    47761 caagaaatac tttttctaaa gctgtttcta ttttttgtgc attttcatat tcacctaagt
    47821 atctaagcat cattactgat gattgaataa gtgctgttgg attagcctta ttttgacctg
    47881 ctatttgtgg agcacttcca tgaacagctt caaatatcgc acaatcttta ccaatatttg
    47941 cacttggaat tatacctaaa cctccaacaa gcccagcacc taaatctgat agtatatctc
    48001 cataaagatt tggcattacc attacatcat aattttctgg atttaaaact aagttcatag
    48061 ctgctgcatc tacaatcaaa tcatcatatt ctacaccatg ttttgatgca acttctctaa
    48121 acacatctaa aaacaatcca tcagacattt tcattatatt tgccttatgt atagctgtaa
    48181 ctttttttct cttattatct ttaacatagt tacatgcaaa atcaacgatt ctttcacttg
    48241 cacttcttgt tattatcttt atactttcag ctgcataatc acctatttta tgttctattc
    48301 cagcatataa atcttctgta ttttctctta caaccactaa atcaacatct tcatatcttg
    48361 attttatccc cttaaaactt ttaataggtc ttaaattgac atacaaatct aatgcctgtc
    48421 ttaaagttac atttacactc ctaaatcctt tacctacagg agtagtaatt gggcctttta
    48481 tagctatttt gttctttttt atactatcta taatatattc aggaagtgga gtaccatact
    48541 cttcaataac tgcctctcct gcattaactt cttcccattc tatttctact ccagtagctt
    48601 ctactacctt tttcatagct ttcgcaactt ctggtcctat tccatctcct ggtataagtg
    48661 tcactttata cattcaatat tccctctttt catattaatt agcttgattt ttagtgtagt
    48721 ttaattttcc accagctttt aatacatcaa cttcttttgc tgacaattct actttgacct
    48781 taaaagaagt tcctttagtt gtattcttaa cttctaaaat tccactacct agattgtcta
    48841 aaatatttgg tatctccaga ttatctaaaa gagatatatt ttcataatct ttctcatcac
    48901 aaaactccat tggaattata ccactattta tcaagttagc cttgtggatt cttgcaaagc
    48961 tcttaactat taccccttta acacctagat ataatggtgc taaagctgca tgttctctac
    49021 ttgaaccttg accataatta tctccaccaa ctataaatcc accattattg tcttttgctc
    49081 tctgaggaaa ttctgtatct acagtattga aacaatagtt tgcaagatac ggtatattac
    49141 ttctaaaagg aagtaactta gcatttgatg gcattatatg gtcagtagtt atattatctt
    49201 ctgtctttaa tataactttt ccacctatag actgacttag ctctgtatta agagggaaag
    49261 gttttatatt aggtcctcta actacttcta catcacttcc tacatcagca ggtttaatta
    49321 tcatgctatc atctatcaaa aattcattta catctaaatt tgtaaattta atatcaaatt
    49381 ctcttgggtc tgtaagcact cccttgattg cacttactgc tgccacttct ggacttacta
    49441 aatatacttg tgctgacaaa gtcccacttc taccatagaa atttctattg aaagttctta
    49501 aagacacaga gtcagtacct ggagattgac ccataccaat acatggacca caagaatttt
    49561 ctagtattct agctccagca cttattatat ctgccaaagc cccatttcta gcaatcatct
    49621 ccataacttg tcttgaacct ggtgctatta ccaaactcac atccttatgt actttatttc
    49681 ctttcaggat ttgtgctact ttcattaagt cttcataaga agaatttgta caacttccta
    49741 ttgccacttg gtctatttta atcttgccta tattttcaac ttcatctaca ttgtctgggc
    49801 tatgaggttt tgcagctaat ggttttaatt catccaagtt tatagtaatc tcttcatcat
    49861 aaactgcatc tgcatctggt tttagctcta tccatgcctc ttctcttcct tgactcttaa
    49921 aaaattctag tgttttttca tcacttggga agattgaagt agttgctcca agttcagccc
    49981 ccatatttgt tattgtagct ctttgtggaa cagataggct tttgacacct tctccaccat
    50041 attcataaac tttacctact ccacctttta cagtttgttt tcttaacact tctaatatta
    50101 tatcttttga tgataccata ttatttaatt ttccaactag attcacctta cagacttttg
    50161 gtgcttttat atagtatgct cctcctgcca tggctaaagc aacatctaat ccaccagctc
    50221 caatagccat catgcccaca cctccagcag taggtgtgtg gctatcagaa cctaaaagtg
    50281 tatctcctgg tgttgaaaat ctttctaaaa atacttgatg acaaatacca tttcctggct
    50341 tagagaaata aactccatat ttatctgcca ctgtttgtat atacttatgg tcgtctgcat
    50401 tttcaaatcc ttgttgtaac atattatggt ctacaaatgc cacacttctt ttagtcttta
    50461 ctttatctat tcccatagct tctagttgaa gatatgtcat tgttccagta gaatcttgtg
    50521 ttaaagtttg gtctattttt ataccaattg aacttccagc aacagcttct ccatctacta
    50581 tatgtttttt aattatttta taaactatat tatctcccat attttcctcc taaacaatta
    50641 tatcttaaat atatgaacct ctaactgctt cttcttctgt tgtgcctttc tcatgtgcta
    50701 gtatatcaca atataattga agaatttctt tatccattaa acttcttttt aatcttatag
    50761 atgtagctct tatagcattt aatagcttgg tagcgttttc tggagaaata tacatttcat
    50821 attcactcaa cttatttaca actgcagctc ttcctgaatg tttacctatt actatttttc
    50881 tttcaagatt tacttcactt gggtcaaatg gttcatagtt atgtgggtct tttaaagctc
    50941 catctgcatg tataccagat tcatgtataa acatattatc cccaactact ggtttccatg
    51001 taggaagtat tcttcctgat gcctgtgcta catactcaca tattcctcta aattttctag
    51061 tatctatatt atttaaatca catttataaa cacacttaag tgccattaaa acttcttcta
    51121 atgctgcatt tcctgctctt tcacctaatc catttactgt aacaccaaca taatttgctc
    51181 ctgctgctat tcctgcaagt gcatttgcag ttgccatccc aaagtcatta tgtgtatgca
    51241 tttctatatc aaaattagtt ctttcataaa gagtttctat tgcattcttt atagaaaaag
    51301 gattcataac tcctactgta tcacagtatc taaatctatc tgcccctgct tgttttccaa
    51361 catttataaa ttcagttaag aagtctatat ctgctcttga agcatcttct ccatttactg
    51421 aaacatataa tccatttttc tttgcaaatt caactgtttt agccatattt tctaaaaccc
    51481 agcctctaga agttctcaat ttattttcta tatgaatgtc agatactgat attgatatag
    51541 ctactgcatc aacaccacaa gaaatagatt cttcaacatc ttttattact gctctattcc
    51601 aagccattat atctgatttt aaatttctac tacatatgtg ctttattaca tttttttcat
    51661 ctccacccat agtaggtata ccaacttcaa tttggtcaac acctaggtca cttaacattt
    51721 cagcaatcat tattttttct ctatttgcaa atacaacccc agctgtttgc tctccatctc
    51781 ttaaagtagt atctactatt tttatttcct ttgctctatc ttttgatata acgcacatat
    51841 taatgccccc cttagtatac ttttaatttt aaaacattaa gtcttatttt tcattgatac
    51901 ctctaactta tgattatttt tttatttttt attcatttta atttcatttg caatttattt
    51961 ttttagcttt tcatatcttg taatctatta tattactttg tatcatttat tgcactatat
    52021 tttttctttt ttgtgaaaac ttcttaaaaa tgaaagttat tatgtttttc ttgcatttta
    52081 atttaatttt taaatagatt tattttcaaa a
//
LOCUS       DAEMGG010000012        50773 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEMGG010000012 DAEMGG010000000
VERSION     DAEMGG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.012"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            169..978
                     /locus_tag="KPK24_000733"
     CDS             169..978
                     /locus_tag="KPK24_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2298440.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKIYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            1112..1351
                     /locus_tag="KPK24_000734"
     CDS             1112..1351
                     /locus_tag="KPK24_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298441.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1539..1715)
                     /locus_tag="KPK24_000735"
     CDS             complement(1539..1715)
                     /locus_tag="KPK24_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298442.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1769..1865)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2220..2894)
                     /locus_tag="KPK24_000736"
     CDS             complement(2220..2894)
                     /locus_tag="KPK24_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG2298443.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(3114..3668)
                     /locus_tag="KPK24_000737"
     CDS             complement(3114..3668)
                     /locus_tag="KPK24_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2298444.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(3746..4111)
                     /locus_tag="KPK24_000738"
     CDS             complement(3746..4111)
                     /locus_tag="KPK24_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298445.1"
                     /translation="MKIRAEYTCLLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4576..5361
                     /locus_tag="KPK24_000739"
     CDS             4576..5361
                     /locus_tag="KPK24_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2298446.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5458..6282)
                     /gene="pdaA"
                     /locus_tag="KPK24_000740"
     CDS             complement(5458..6282)
                     /gene="pdaA"
                     /locus_tag="KPK24_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG2298447.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            6503..9622
                     /locus_tag="KPK24_000741"
     CDS             6503..9622
                     /locus_tag="KPK24_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG2298448.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FLEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKYRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            9640..10239
                     /locus_tag="KPK24_000742"
     CDS             9640..10239
                     /locus_tag="KPK24_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG2298449.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10496..12625)
                     /locus_tag="KPK24_000743"
     CDS             complement(10496..12625)
                     /locus_tag="KPK24_000743"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG2298450.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            12891..13139
                     /locus_tag="KPK24_000744"
     CDS             12891..13139
                     /locus_tag="KPK24_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298451.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            13305..14696
                     /locus_tag="KPK24_000745"
     CDS             13305..14696
                     /locus_tag="KPK24_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG2298452.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            14811..16280
                     /locus_tag="KPK24_000746"
     CDS             14811..16280
                     /locus_tag="KPK24_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG2298453.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            16345..16938
                     /locus_tag="KPK24_000747"
     CDS             16345..16938
                     /locus_tag="KPK24_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG2298454.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            17120..18466
                     /locus_tag="KPK24_000748"
     CDS             17120..18466
                     /locus_tag="KPK24_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2298455.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            18648..19019
                     /locus_tag="KPK24_000749"
     CDS             18648..19019
                     /locus_tag="KPK24_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG2298456.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            19012..19701
                     /locus_tag="KPK24_000750"
     CDS             19012..19701
                     /locus_tag="KPK24_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG2298457.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            20221..21087
                     /locus_tag="KPK24_000751"
     CDS             20221..21087
                     /locus_tag="KPK24_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2298458.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            21329..22138
                     /locus_tag="KPK24_000752"
     CDS             21329..22138
                     /locus_tag="KPK24_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298459.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            22282..23346
                     /locus_tag="KPK24_000753"
     CDS             22282..23346
                     /locus_tag="KPK24_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG2298460.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            23374..24093
                     /locus_tag="KPK24_000754"
     CDS             23374..24093
                     /locus_tag="KPK24_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2298461.1"
                     /translation="MTSWKKKTVYKCLIAVALFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            24117..24701
                     /locus_tag="KPK24_000755"
     CDS             24117..24701
                     /locus_tag="KPK24_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG2298462.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            24703..26052
                     /gene="pabB"
                     /locus_tag="KPK24_000756"
     CDS             24703..26052
                     /gene="pabB"
                     /locus_tag="KPK24_000756"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG2298463.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            26042..26785
                     /locus_tag="KPK24_000757"
     CDS             26042..26785
                     /locus_tag="KPK24_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBG2298464.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            26819..27520
                     /locus_tag="KPK24_000758"
     CDS             26819..27520
                     /locus_tag="KPK24_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298465.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKILNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            27546..28118
                     /gene="folE"
                     /locus_tag="KPK24_000759"
     CDS             27546..28118
                     /gene="folE"
                     /locus_tag="KPK24_000759"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG2298466.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            28182..28973
                     /gene="folP"
                     /locus_tag="KPK24_000760"
     CDS             28182..28973
                     /gene="folP"
                     /locus_tag="KPK24_000760"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG2298467.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            28984..29349
                     /gene="folB"
                     /locus_tag="KPK24_000761"
     CDS             28984..29349
                     /gene="folB"
                     /locus_tag="KPK24_000761"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG2298468.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            29342..29848
                     /gene="folK"
                     /locus_tag="KPK24_000762"
     CDS             29342..29848
                     /gene="folK"
                     /locus_tag="KPK24_000762"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG2298469.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            29829..30614
                     /gene="aroF"
                     /locus_tag="KPK24_000763"
     CDS             29829..30614
                     /gene="aroF"
                     /locus_tag="KPK24_000763"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG2298470.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            30869..32659
                     /locus_tag="KPK24_000764"
     CDS             30869..32659
                     /locus_tag="KPK24_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG2298471.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            32677..33843
                     /gene="rpoD"
                     /locus_tag="KPK24_000765"
     CDS             32677..33843
                     /gene="rpoD"
                     /locus_tag="KPK24_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG2298472.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            34008..34697
                     /locus_tag="KPK24_000766"
     CDS             34008..34697
                     /locus_tag="KPK24_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2298473.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            34711..35808
                     /locus_tag="KPK24_000767"
     CDS             34711..35808
                     /locus_tag="KPK24_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG2298474.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            35871..36443
                     /locus_tag="KPK24_000768"
     CDS             35871..36443
                     /locus_tag="KPK24_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG2298475.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(36503..36970)
                     /locus_tag="KPK24_000769"
     CDS             complement(36503..36970)
                     /locus_tag="KPK24_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG2298476.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(37356..37832)
                     /locus_tag="KPK24_000770"
     CDS             complement(37356..37832)
                     /locus_tag="KPK24_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG2298477.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(37952..39043)
                     /locus_tag="KPK24_000771"
     CDS             complement(37952..39043)
                     /locus_tag="KPK24_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG2298478.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(39036..39425)
                     /locus_tag="KPK24_000772"
     CDS             complement(39036..39425)
                     /locus_tag="KPK24_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2298479.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            39617..40063
                     /locus_tag="KPK24_000773"
     CDS             39617..40063
                     /locus_tag="KPK24_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298480.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            40327..41052
                     /locus_tag="KPK24_000774"
     CDS             40327..41052
                     /locus_tag="KPK24_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2298481.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            41039..42283
                     /locus_tag="KPK24_000775"
     CDS             41039..42283
                     /locus_tag="KPK24_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2298482.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGQGTEFHIIFHKMYDSD"
     gene            42363..43040
                     /locus_tag="KPK24_000776"
     CDS             42363..43040
                     /locus_tag="KPK24_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298483.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            43041..45602
                     /locus_tag="KPK24_000777"
     CDS             43041..45602
                     /locus_tag="KPK24_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298484.1"
                     /translation="MNLYTLLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINNSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIITIVSIIIVLLTIF
                     ISILLPAISASNISHLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            45823..47097
                     /locus_tag="KPK24_000778"
     CDS             45823..47097
                     /locus_tag="KPK24_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2298485.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(47258..50299)
                     /locus_tag="KPK24_000779"
     CDS             complement(47258..50299)
                     /locus_tag="KPK24_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG2298486.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            50506..>50773
                     /locus_tag="KPK24_000780"
     CDS             50506..>50773
                     /locus_tag="KPK24_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG2298487.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYN"
BASE COUNT        20323 a         5373 c         8408 g        16669 t
ORIGIN      
        1 tatacctaat attacaatat aaaaatattt taataattaa ttattaggat gtttattttc
       61 ctatccttgt tttaaagatt tttttagtat attctaatac tatagtatta tagagtaata
      121 atgttgattt aaaataaaaa taagatttcg gaggagttta ttatataaat gaaagattac
      181 tataaaatag gagagatatc taaaatatat ggaataggta gagattcatt aatgtattat
      241 gaagaaatag gcattctaag accagttaga gatataaatg gatatagaat gtataatata
      301 tcagatatat ggaaattaaa tttaataaaa gaatttagaa gcttgaactt tccaatgaag
      361 aaaataaaag aatacctaga tgacagaagt atagagtcta cgaaaaatat tttaaatgaa
      421 gaactcgatt taattgataa aaaaatagct gaatttataa gccataagga aaatattata
      481 aaaagattga gttcaattga aagtgtaatt caaaatacaa aaatagatga gatagaggtt
      541 gtatatatag aaaaaagaaa agctttagaa ttaaatgctg atataaagag ggatgaagac
      601 ttcgattttt taattcaaaa acttcagaaa gaatatgaag atagatttaa tatacttgga
      661 aacaacaata ttggttctgc cttttctact gaagcaatta ataaaggtat atttaatgag
      721 tttaaatctg tattttgttt tctggaaagt aatgagaaag tatataatat agtttttgat
      781 gaaggatatt atgtaacttt aaattatact gggagtaatt ctaataataa gatatatatg
      841 gagaaggtgt ttaaatttat agaagaaaat aattataaga taattgctga ccctatagaa
      901 atctataaga tagatataca tgagactgga attgtagagg agtttgtaac ggaaattcaa
      961 gttcctataa ctaaatagct atatttcaat ggattagatg gtaaatagaa tgaaaaaatg
     1021 ttgatttgcc actgtgttat aaaatcggta gcaaattata ttttaaatta taagatagca
     1081 tgaaaaaata attgtttagg gggttataaa tgtgaaatgt tctaattgtg gaagtgtaaa
     1141 tattgaaaag ggaattttaa caactggtgg catatatgct gaaactattg gattaaaata
     1201 tagaggagat ggaaaattag ataaagttat aaataaaact tttccagtac aatctactat
     1261 atattcagat ttatgtttag attgtggaga aatagttaga acctatataa aggataatac
     1321 tgataggaat tggtgtaaag ataagaaata gaataatttt tgaagtatat taatatttga
     1381 agttgaattt tattaaagat acaagtgaaa aagtataaat gggaagaaat tgttatatgg
     1441 aagaaaactt tgcatatagg ggagattagt tttttaatat aataacattt ttaaatgtag
     1501 ataaaactaa aaagggttct ctagaaaaca atttctattt aattatactt acttaccaat
     1561 accatcactt gatgtaaaca agattctact acattttctc cgtactctag tataaaatca
     1621 tctagctgtg tatctataaa atcacaataa tcatataaac tactgatata agcagttaac
     1681 atgtgtgtac atttatccat ttcatctcta aacatatata ttactatccc cctaaaaaac
     1741 tttcataaaa caaaaagaac aatatctttt tggcaactgg ctgacataac tcatagttta
     1801 ttttataagc gttagtccct atagctttgc gtcactaaat ttctctagtt ttgccgattt
     1861 agttttattc tacaactaaa ataatacact aggataagtt attgttcaac atacttgtca
     1921 gaaaattgaa atatttaaca gtatcttaac aatatgaaat ctatttttca ctcaagaaat
     1981 aaactataca tgtagaatat ttaataaaaa ttatatttag ttttgtattg aagtttttgt
     2041 attaaaaagt ttagttcaca agagttactt ggctagataa tagatgaaca aaaatatctt
     2101 gaaaaaatat atatgaatgg caaaaaacaa gggatgaatt ttatatagac tcatcccgta
     2161 attatttata tattgacctt gattaaatat attattattt ttaagcttca atttttatat
     2221 tatatttcta aagtttctat aagaggatta tcgctaaata attttaataa atttgttttt
     2281 ttatatcctt taggtaactt agtatatata actacttcta accaatcccc atcaaggcgt
     2341 ttaatattca tgtttataat ttcaatatta gattttttaa atttaccttc aatatattct
     2401 atggaatcag tgctgttaga aattttaaga gaaacttgct ctgctgtagg gagtttcatc
     2461 catttcctat ctctatgaag tgttatctgt acaataacaa taatgatact tgaaacaata
     2521 ccaactaagt ataatccaga gccaatggca agacctgtac cagctgttgc ccatatacca
     2581 gcagctgtag taagaccact tactgattga tttctaataa atattaatcc agcacctaaa
     2641 aaacctatac cactgacaac ttgtgctgca acgcgggctg ggtctagacc aatacctgga
     2701 gtacccacta tatcattaaa tccatacttg gaaacaatga ttataagtgc tgaagctaat
     2761 gccactataa aatgagtgcg tattccagct tcttttagtc tattctttct ctcatatcca
     2821 attaaagccc cacagaaacc agcagtaatg attcttgcta tatattcaaa ttgaatcaat
     2881 atatgatggt tcatttatct agcaccccct tctatataat attatatata ctataatact
     2941 acatttttta tgtgaatata gtaaagttta aaatttatat aatagttata tatgaatata
     3001 aaatgtataa acgtatatta tgtatataaa aatgatagaa agcatctaat tttaaatgat
     3061 taaagagcaa gcttaaataa atcaaacttg ctcttttaac atagtgtatg tttttatttg
     3121 tgatagttag ataaaaattt atctgtgttt tttacatctg caaaactgtt ctgtagtgat
     3181 gccatgaata cttgatgtac agtatttgca ttaatttcta tattattcca agataaattc
     3241 ttcgttgtac atgcatcgct tattagagtt atgtcgtatc gaagtttctt tgctgttctt
     3301 acacttgtat caatacacat atggctcatc attccacaga ttactagatt agtaatattt
     3361 tttttctcaa ggatactttg caaatctgtt tcaaagaaac tatctggtgt gtgtttagtt
     3421 ataatttctt cattatctag tggaaaaaca attttattta tttctgaacc atgagtatta
     3481 ggaagaaaaa aagaagctgt tttatctgtt gatatatgct gtatatgaat aactggtaaa
     3541 ttgttttttc taaaaaacag tagtattttt ttagcatttt cagcagtttc ttctgattga
     3601 aataattcac attttccatt tttaaaataa tcattttgaa tatcaatcaa tattaaagct
     3661 atagacatga ttatacctcc tatttatttt tattataata ctattgtaaa aaatgtaaat
     3721 tacttacttt ttggtatgta taggattact tattgtagca aattccttta ttatctagaa
     3781 tttcttgttg accatgttca agcatatatt cgattcctat ttcttgcatt atttccactg
     3841 cttttaacat ttttttacca cgattagtaa gaaaatattc aacatgtaat ggatatccag
     3901 cagaacttat tttatcaata atcccaaaat cacgtaactc ttttaattgt tgaatcaaca
     3961 ttttttgatt tattccataa atattatgtt gcaattcaga aaatgtttta ttagattgtc
     4021 ttaattcaaa tataaggata gttttccatt ttcctttgat tatgtcatgt acaatttcaa
     4081 gaagacatgt atattcagct cttattttca tagcatatca cccccagtaa aatgataata
     4141 aattatatca cttgtcttgt gatatgacaa atatatatga actgtatata aattttatgt
     4201 tttattaagt tctaaatttc ttctattgta gttcatattt agtacataaa taattagtaa
     4261 gatataagta aataaatata taacatgact atagaaattt aactttaaaa taacttatga
     4321 taatttagag gaggaatata tatgaatata tcaagaaaag ctatgaaaat aatagaatta
     4381 gcacaaaaaa ttgcaaataa gagaggtatt tcagtagaag aagcttggag tgaagcagta
     4441 acagaatata aaaataaata cgaacatatt gcatagatat ttaaaaattt agaatatttg
     4501 gtagaatata atgattttaa atgatactat aaattataag gtatatcatt attaacatag
     4561 aaggaggata tctgcttgat tgtaaaaata gataatactt tagaaaaaga attttggcaa
     4621 tatgtatcac atgaagaaag tttaaattta ttcataattg gatacgttga gaattatgga
     4681 tttagctctc aatatcaaga tatatggtct caggtagaag atggaaatat aacctctata
     4741 atattaaaaa ataagtcaac acttataatt tatagtttta aaaataattt taatatagga
     4801 gaaatgaaga atcatataaa agatttagat gtagaaagca taagtggaaa aaaatgtgta
     4861 atagatagac taataagtaa atacaaagat ttttatgaaa aactagataa taaattttgt
     4921 gtattaaaag aaataaaaga aatagatttt tccaacatga aggagtataa aatagaaaat
     4981 gctcaggaaa aagatattga tgaaatagga aaacttttaa ataggtcaga ctataaagta
     5041 agtaaaaatt acatagaaga aagaaaagtt catttaaaag agggaaatgt aagagcatat
     5101 tttattagaa atgatgatac tatgattagc actgtgtcaa ctggtatgga aactagtttt
     5161 ttggcaatgg tggtctctgt aagtacagat aaaagatata ggggaaaagg acttgcaagt
     5221 tatatggttt acaatttgag taaagaattg ttgttagaag gaaaagttcc atgtcttttt
     5281 tataataatg atgtagcagg taaaatatat cataatatag gatacaaaga gataaatgaa
     5341 tggacaattc tttttaaata gctataaaaa ataaaaaact ctatcgagaa ttaaagtatt
     5401 tataaataac tatattttta ctataattca tgatagagtt tttttaaatt tatgatacta
     5461 ttttttatta tttaaataat caagtgtttt aagtttataa ccttcttttt cccaatgtgt
     5521 aagaacctca tctaatatct cagtatttgt tgatgaattt ggatgcagta aaactattgc
     5581 accaggatga gttcttgaat aaattttgtt tttggcaaat tcatgagtag gttgtttctt
     5641 ttcataccaa tctacataag caaaactcca aaatattgat ttatatccta aatcttgtgt
     5701 atattttaaa gattgttcgc taaatttacc cattggaggt ctaaaatact taggcatttc
     5761 ttttccagta acatctttgt atgctttttc tacacttgta atttcttctt tgaatttttc
     5821 aaaatctgta atacctgcca ttgatggatg gctttttgaa tgattacaaa ctaaatgtcc
     5881 ttctttttcc atacgcttta ttaactctgg attgcttttt atataacttt caactacgaa
     5941 aaattgagct ttagcattgt gttttttaag cgtatctaat aattttggag tgtttccact
     6001 ttcatatcca gcatcaaatg atagatatat aactttttca ttgggatttc ctacataata
     6061 agctccgtat tttttaaaaa aatcagcttc ttttataggt gatggctgtt taccatcctc
     6121 cctagggtta aaataccaat catattcatg tgtgtcgagt gttggactag aaatttgagt
     6181 ttttgtttta tctaatgatt taaaatttat actagcaatt ccaaaaagta ttagagcaaa
     6241 tgctcctatc ataatgtatt tttttaggct atcttttttc acaatttcac ctcttattta
     6301 aaatttaaag tatatttctt ataatatagt ttgaatattt atagttaaaa atattcacat
     6361 atacaaataa taaatgaaag aagtttactg taagatataa agttgatatt aattatctta
     6421 aataaagtgt atataaatga taaaatataa agagaaatga ttgtaaataa gttaggctgg
     6481 tttaacttga agggatataa aaatggataa tataataagt gtattgaatg atgttgtttt
     6541 tttagatata gaagttagtg gattagattg tttaaattca gaaattttag aagttggagc
     6601 tgtcaaggtt aaagactgga aaatatatac ctatgaatca ttaataaaaa acaaatttga
     6661 ggtacctgta gaagtattct cagtatgtaa aaatttagat aaaaatgatt tagaaatagc
     6721 taatgaaata gaactggtgg aagatagact tgtaaatttt gtagaagata gtttcattat
     6781 atgtcatgat ttaagtctga aaaaaaagtt tcttgagtat catatgccaa agttaaaaaa
     6841 caaatttatt gatttaattg aattagcagt tattttagaa ccatatcata aagattatag
     6901 cttagaatac ttaaaaaata ctttaacaaa ttgtaattct aaagtggaaa atagagcttt
     6961 atcagatgct attgatataa taaatattgt aaattgcctt ttagtaaaat ttaataacta
     7021 tgaaaaaact actttggagc cattgagttt taaaataaat tcatatctta aaaaatttaa
     7081 tcttccaaca tgggaatgga gcaaattttt agaagaggca aattatgatt tatcaaataa
     7141 tataaatata aaaaaagaat ataatatatt tgactcaaaa gaagaaaaga agaaggaaag
     7201 agaaacctta aaaattttaa atgaagaaga aaaaaattat gaagaattat taaaatacaa
     7261 aactatttgg gagaataaag aaggatttac atatgaatat agacctggtc aatatgagct
     7321 tactaaaaca ataagagagt tgtttagaaa tagcgaagat gaagaaaaaa ttgcatgtat
     7381 agaggcacca actggtatag gtaaaagtgt aggatattta ttgcctgcaa tattagaggc
     7441 tagaattaat aaaaaaagat tattaatttc tactgacaca aaggaattgc agatacaatt
     7501 aataaataaa gatattccaa atgtgttaga ttctttgggt ctaaatggaa aagtaagtta
     7561 cggatatata aagggaaaaa ataactatat ttgtatagat agactggaag catatataga
     7621 tgattatgag tctcaaaacc ctactaaagg agaaatttta tctttaatat ttttgaagag
     7681 attagttgaa ggtggcaagt atggagatat agaagaaata aattatttag tgtttgacaa
     7741 cttcaaggaa ataagtactc acttaagaaa tgtaagttgt gacccaaata tgtgcagacc
     7801 taaaaaatgc aaaaaagatt gtttatataa aaacagaata gaagaattaa aagaagaaca
     7861 tataacagta gtaaatcatt cactattagc taaatggcca tataaggatg aaaaaccttt
     7921 agaaaatata atagtagatg aagcacataa tttgactgaa aaaggatatg atttcttttc
     7981 aagcattata aattcaaagt ctttaaggta tttattacaa gaaatttatc cttacgagtt
     8041 tattcaaaat agtagcttta tatataaaaa atatagtaga aatatgagaa aaataaaggc
     8101 ttttgataaa ttttataatg tattaaaaat tggaagagaa gataaacaaa aaattgctag
     8161 aagtataaat ctcattattg aagagataga ttcaatatta aagtttggta attgtaatga
     8221 atataataat gtaagtaatt ataatttaag atgggaactg aatttgcaaa tagacgaaat
     8281 tgtgggaaaa ctaaaaaagg atggtataga tactgaaata agttatagag cctatagtga
     8341 aaagataaag ttgagttgtg agaaaataat aaaaaattta gtttcaataa taataataat
     8401 ttataggaat attgatgatg atagtataga taaagaagca gatatatata aatttggaaa
     8461 ggcaaagaca agagatttag aggatataaa gattatattt gaaatctttt tagagtatga
     8521 tgaaaaggat gattatgcaa ggatagttga aattgataag aattataatg actttgaatt
     8581 tcgagtagtg ccattaaaaa tagcagattt atttgaagaa aatattttat ctcaattaga
     8641 aaaaggaata tttttatcag caactttgag tttatctgaa agtatgtctt attttaaaaa
     8701 tacattagga atagacagag ttaagaatgt ggaaaagatt atagaaccaa tttttgatta
     8761 taaaaataga gtctctgtag ttggattttc tgatatttgt gagtacagaa attctgaatt
     8821 tccaaatgag atgagtaaaa ttatatctaa tatatcaaag attacagaag gtcatacatt
     8881 agctcttttt aatagtaaag atagacaaga aaaaacttat gaaatattga aaaaatactt
     8941 acatagtttt aacttagaaa tttatgctga taaaaagggg attagacacc ttaacgattt
     9001 aaatagaaaa tgtgtagtac ttggctcaaa aggatgtttt gaaggcgtag acattccagg
     9061 ggatggtttg gtttgtgtaa ctttagacaa gcttccgaat ctaaatccaa aagacccttt
     9121 gtattttact ataatgaaga agtatggtat agactactat actataaatt atcctcaaat
     9181 gaccataaag gtaaagcaag ctatggggag aattttaaga agcaaatatg actatggatg
     9241 ttttgtgatt tttgatgtag gaactaatat aagtgtgtta aagagattag agaaagattt
     9301 acacgattgt aaaatttcaa aggttaatag caatgagttt tatacttata taaggcgaca
     9361 tttaaataaa tatagaagtt taatactaaa atctgttata tttgatacta taaaagcgtt
     9421 gaatgttgat gctaaaatgg ataacaatga tgttgataag gatattataa aaaaagatat
     9481 aaatgaaaat ataagacaaa gagctgtaaa aggagaagtg taccatattg atattataaa
     9541 aaaagatatg aaagtaaaat attttgatag aaactatctt ataaatttag atatatttat
     9601 gagagaagaa gataaaaatt aagagtggga gtgatatatt tggcaaaaca aaagttttat
     9661 gctgtgaaaa aaggtaaaaa tataggtgta tataatacat gggatgagtg caaaaaacaa
     9721 gtaaatggat tctcaggagc tgaatataag agtttttcta cttttcaaga agctaaagaa
     9781 tatattgatg ggtcagaaaa attgagtttt caagaagata aggagtttat agaagcatat
     9841 gtagatggaa gttatgaaca ttcagtaaaa atgtatggtt cgggtgttgt gattttaaaa
     9901 aataatgaag ttataaaaac ttatagtgaa aaaggaaaag agaaaacttt agtaagtatg
     9961 agaaatgtag ctggagagat agaggcttct aaaattgcaa tgcagtactg tattgacaat
    10021 aatgttcaaa acttgatttt atactttgac tatgaaggga ttgaaaagtg gtgtactgga
    10081 gtttggaaaa caaataaaga aggaactata gcatataaaa atttctatga cagtattaag
    10141 aataagctta atgtaaaatt tacaaaggtt aaagctcatt ctggaaataa gtataatgag
    10201 gaagcagata aattagctaa aaaagctatt ggtgtataat aaaaaaatat ttatacataa
    10261 ttacatggta taagagtata tctaaattcc tactttataa ttaaacaact tgtaattaaa
    10321 caacttgtaa ttaaacaact tataattaaa taaattttga ttaaaaaagc agattgtatt
    10381 atgaaaaaat aagtatctaa tttataaaca attaataata gtattaatta gatacaactt
    10441 atttaatata ataaaatctg ctttttatat aactataaaa ttttcctaca ttctattaga
    10501 attgcccata aataccttca agttcttctt ttaatacttc agggtcattt ataccaatag
    10561 acatggttct agccagtata tttgcttggt cataagttga tgtccatgag aatacaccac
    10621 ctagacaatt tctaagaaca tattgagttt ttaagaatat tgataaagca tcatcatatg
    10681 acattgcaaa atctccatcc ttaactaaat atggaacttg agcagtatta tcaaatctat
    10741 attcatatcc atttttattt atataatccc ttctaagctc atcataagtt cttgttatag
    10801 tagcacctaa tcttccataa aatggtatac caagaaggat tttttcagaa ggcattccag
    10861 cattctcaag atttcttacc attgcatcaa cactatatcc tggcaaagat aagtctgaat
    10921 caaaaagatt tgcttgatgt tttctaccat ttgggcctgt ttcacctgct gtaaaatcat
    10981 aactcataag attaaaataa tctattatag gagctatttt atctatttca gcacttgaat
    11041 ttatatatcc tctatctcct gttccagcta cactaagcca tttatcatct cctataacat
    11101 ctcttatggc agttagtaag agtgtaaagt tttctctatc ttgaggtctt gatgttattc
    11161 cagatgcact acttccagga tattcccagt ctatatctat tccatctaaa gcatattcat
    11221 ttatcatctg attgacctgt cttgcaaaat tatatctaga tgtaggtgtt aatgctgcat
    11281 cagagaatcc ttcagctccc caacctccaa tagctacaat tacttttaag ctaggttttt
    11341 cacctttaag agcaagtaat tgatttaaaa atctaggata aggaataaac aaatttccag
    11401 actggtctat ctcagcaaaa gcaaatataa ctgcatctaa tagttgagca tctacattag
    11461 ttggatttcc aagaacatat tctgctacaa ttggatttat atctggacag tttgcatttt
    11521 caactattgg attgtatggg tctgttattt gtggtctaac taatttttta gaatctttta
    11581 aagttttcat agctattttt ttagtatttt tttcgctagt ataatcgtag tttttataat
    11641 tatgtttata gaaatcttca taactactat cttctttgtt atagctagaa tttattttgt
    11701 cacaagattc atctttatca tagttaaaat tcatttcatc acaagactta tctttaaact
    11761 tatcttgttt ggagttgtga actttgttag tatttttagt atgttttttc tcatgattgc
    11821 aatcatcttt aatatcagtc ttgtgttctt gtttacagtc aagagctttg ttcaggtagt
    11881 catgatgttc atgttcacag ttacattcat tttcaatgtt tttagtatgt tcttttttcc
    11941 agctacaagt attatcaatt ttcttgctca cttcttcatc attataatta tctggtacaa
    12001 aacataagta ccagtccata catgaatatt tcttattaca actgttaact ctatttttta
    12061 attccttata gtttttagga taaggtaaaa tcactggcat tccaggaaac cagtttgcag
    12121 gagtaactat attatctcta tcactagtct gaagtgcatc tactattcta agtatttctg
    12181 gaatatttct tcctgtagtt agtggataat aaagaatcgt tcttagaatt tgattattat
    12241 ctataataaa tacagagcga acagttgatg tatcactcat tggttttgat atcatgccgt
    12301 ataacttggc aattctcata tctctatctt ctataatagg aaatggaatt tctacacctg
    12361 taagtaaaga aatattatac atccaagcta aatgtgaact gttactatca acacttagac
    12421 caattagttc tgtatttctt tttttaaatt cgtcataata tttagcaaaa cataaaaatt
    12481 ctgtagtaca aactggtgta aaatcaccag gatgtgaaaa taaaacaatc caattaccct
    12541 tatagtcaga gagtctaata ggaccatttg ttgtattggc tttaaaatca ggagcctttg
    12601 accctaaact tggcaaattt ggcatgtaaa tcacccctca ataaattatc atttatttta
    12661 ttctattaat taatacatat tagtgttact attttattaa aagttaagta atccttatgt
    12721 caaaaaaaga tatttattaa aaagatttaa tattttccta atataaatga caatagtaat
    12781 tgctattttt ttactaattt ggtatagtaa tggataaggg gtagtgtcaa ttttagtgat
    12841 tatacgaggt gagtggattg aaaaaaattc taagaatttt agttatctcc atgatgatat
    12901 gttttgcaat gacttttgca ttagtgttaa taaattcagt tatgggagta catataggat
    12961 tggattatta ctttttgatg ggaagtgtgc ttacagtttg tttaacaatt ggaattgttt
    13021 tatgcaatgc tagaaaaata tttgatagca aagctataca caagacaaga acgaaaactg
    13081 tcagtaataa taaaaaacaa aagagtcatt ctagggatat aaaaagaaag atatcataat
    13141 ttatgtaaag gaaatattta tgtataaaat aatgattgat aggtaaaata aaaacacgat
    13201 ttaacttgtg tttttatttt ttttcaagaa ttatgatata ataaccaata agaaatttag
    13261 tttaccataa aatataaaga ttattaaatg gagagggaac tataatgaat tttagagatt
    13321 tatttgagaa ggctgtagat tttattgatg aaaaaaggaa atctatagtt gcgatttcat
    13381 tatcaacatt aggtgtaatt gctttgataa tagtattttt cttaagtagt aacgagttta
    13441 gtgtaggaaa tgaagctaat gagttgttaa agattataga gaaaagacag tattctatag
    13501 ctgttgatta ctacacaagt atagaaaaaa agttttctga ttcaaaaatg gaaaggttta
    13561 ataagtctgt ttctaaaaaa ataaataaat tgctattaaa tagtggtgat aaatatttag
    13621 atggagacat aagtcaagaa agttttattg gattaataaa taccgtgaaa gaattaaaca
    13681 aaataagtat agatgtggat gacttattag ctcaagctga tagagttaga gaaatgtata
    13741 aagaagaaaa tactacttat gatattgcca ttaactacat aagtactgtt tcaatattaa
    13801 atggtatggg tagtaaccta gatgtataca aacagaatat agaaacaatc aaagagtcta
    13861 gagatgttta tgactcagca gtaaaagacc aaaaagcata taagtattat gaagctattg
    13921 aaaagtataa taaagtatta aaagaagatg aaaagtatta cagtatggct caaaatggga
    13981 aagaacaatg tattgaagaa atgtatgatt actatataag tagagctgaa gaggcaaata
    14041 cttctggaga ttatgaaaga gcattacaat atattgagta tttaaaagac gattattcag
    14101 atgatgaaaa ggtacagagt ttagaaaaaa aatataaaaa gaatttatca ttgtatactt
    14161 taacatctga tgacattata aatgtaatag ctaaaaaaag tggcaaagac aaggcaaatc
    14221 tatctataaa ttcacttcca caaatgataa aaaataataa atactattat gctgaagtat
    14281 atgagtatga taaattaata aatgaagttt tggtaagtgc aaaaactaaa gatttatatt
    14341 catataaaga tggtaaaaaa gattacaaag tagattatgg tgatggatac tttagaatat
    14401 tagaagatgg aagttatcaa tttggtatta caaaagataa agctaagttt gtgttaacta
    14461 atactcttga tgaaaaagaa aataagtata aaaagattga gatattagat atagagaaag
    14521 cagatagata tgtaaaaagt aaaaagagtt tagaagaatt gtttggaaaa tataaaaata
    14581 tttattatta tgctgtagta aataaaggct tatttagagg caaagaagtt tatgctatta
    14641 atatttacaa tgaaaaaata tatgccatat cagaaaatgg actaaatgaa tattaattaa
    14701 taatattaaa ttaatatata attaaaaagt ttttggggtt ttaaggcgca ttatgtagta
    14761 atataataat agggatatat aaataatttt attgtaagga gaagtatggg atgagtaatg
    14821 tgaacaaaaa gctagttata gctattggag ctgtagcatt agtagtaata atttttatag
    14881 gcataatgtg catgcaattt aaaggggaga aaatagcaaa aaacacatat gttaatggag
    14941 taaatattgg aaaattaact aaatcacaag caaaacagga attagctaaa aaatacaaat
    15001 tagagaatgt tgattttaat tacaatgata aaagctggaa agtaaagagc aaagatttaa
    15061 atttatctac tgacctggat aaaacagtag aaaacgcata taatttaaat agaaagagtg
    15121 ctttctttgg caatctatca aaaactattt cagcaaattt tggcaaaaaa agcaacttag
    15181 tagttgcgat aaattatgat aaaaataaat taaaagcaga aatggaaaag atagctaaag
    15241 aaatagatgt agatgtaaaa gatgctacac ttgatataag tggtgaaaaa gtcaaggtta
    15301 taccagattc tgatggtctt aaaatggata tttctaagag tatggagaat tttgataatc
    15361 aaactaaaaa aggtaactat aaaaatgagt tagtagtaaa agctactcca gcaaaagtaa
    15421 aaaaagagca acttgctaat atagatacaa atttaggtac gtattcaaca acatttaaaa
    15481 cttctcaaat aaatagaagt ataaacataa aattagcaac agataatata agtaatgtat
    15541 tacttatgcc aggagaaact ttctcattta ataaacatac agggaaaaga agtaaggaaa
    15601 atggatataa aagtgcacct gtaataatgg aaggtgagat ggaagaagat tatggtggag
    15661 gagtttgtca agtttcatct acgttataca attctgtgct atacgcaggt ttagaaattg
    15721 ttaatgtaaa aaatcatact ataccatcaa gttatgttcc aaaaggaaga gatgcaactg
    15781 ttgcagatag tggaatagat ttcttattta aaaataatct aaagcatcca gtttacataa
    15841 aaaattacgt ttcaggaaat caaattgttt gcaatatata tggaagtgca gaagataaac
    15901 aaaatataac aatatcaaca aaattagatg gcgtttcaca aactacaatg aaaagagtta
    15961 atgaccctac aatgcctaaa ggtaaagaaa aagtagacaa aagtggaaga aatgcatatt
    16021 ctgtatcaac atatagaaca tttaatgatg caaatggtaa aaaaattaaa actgaaaaaa
    16081 tagctaattc atattatcct aagaaggaag gtattatatt agttgggact atggaaccaa
    16141 aaccagagga aaaaccaaat acagacgaaa ataagaataa tcaaaataca aacaatcaaa
    16201 acccaaataa tcaacaaaaa ccagaaacac caccaactga taataagcct aatgagacac
    16261 aaccacaacc gcaagcttaa agttctcaag aataggattt atgtagagta ataaaaaata
    16321 taaatctaaa taggagggat tactttgaat ttaaataata taacgaatta cgttcaaagt
    16381 atttgcgaga atatttctaa tgtcttggat gtagatgtta ctttagttac aaaagattta
    16441 actaggatag caggtacagg aatattcaga ggtaaaatag gagagacaat atcagagaag
    16501 tcagtgtact ctcttgtttt aaaagatggg aaatcctatg ttataaataa agaaatagaa
    16561 gagaactgta ataaatgtgc aaatagagaa aattgtaaag agttagctga tatatgtact
    16621 cccgttaaaa tgggagagca tatacttgga atcttgggta tagctgcgtt taatgaagaa
    16681 caaaaagaaa agatactttc aaaagataag gagttaactg cttttgtaag gagtatgtct
    16741 gatttaatat cttttaaact agatgaactt tataaacaag aagttaagac tatagatgac
    16801 cttgagaggg aagctataga aaatgccata aagaaatatg gaagtaatac agaaggtatg
    16861 aaaaaagtag ctgaggcatt aaatataggt atagctacac tctatagaaa agtaaaaaaa
    16921 ttcaacatta aaagttaata gtattgaaaa agaggtaacc tttttatgaa aagaagagga
    16981 ttaccttttt tattatgaga atggtggaaa aatatgtata gttttgactt gacatattaa
    17041 atatgttatc ttaaatatat attttagagt ggaggtgagt attattttag ttaaagatat
    17101 aaaacaagac atagaaagca tgcctagaaa aattttagat atatctttga tagaagataa
    17161 taaaagtgat ttgacttttt ttaatataga agaagaagac ggtgatgaaa cattaaaagc
    17221 attaaaacag agttatgtga ctacaaatta taaagaagaa actctattaa acttgcaagg
    17281 agctttaaat tcaaagtcag atacaacgtc taaggagtta aaattagaat catataaaag
    17341 atataacaat gcattggatt tagcatataa aaattatata actagtgcca ttaatatggt
    17401 taataaagct ctggagataa atcctaaaga cattgatata ctaaatctta gaggtctttt
    17461 aaaattatta aaatgtgatt ttgctaaatc atttgagagt ttttatactg ctttatgtta
    17521 tgaaaataat catatatcta gaaaatatgt aaatctaatt tcatctgaag attttaaaac
    17581 ttttttagga agatacaatc attctataag atttataaat gaagaattaa attatgagtc
    17641 aatacatatt ttagagaaca taattgaaga agaacctgaa ctaatagagc catatgtaat
    17701 tttatctttg ttatatgata aactaggtaa tgtaaaaaag agagaaggat atttagacaa
    17761 acttaaagag ctagataaag ataatcctgt atttgaaaaa aatgaagaag aaaaagaaga
    17821 tacaagtaaa aaggaagaaa taaaaaaagt aaaaagaaag aaaagtattt tgccatatat
    17881 tatagttggc tgtgttttaa taggtatggg gatatatttg attcaaagca aaaagagaat
    17941 agaaaacttg aataaccaaa taagtagtaa agaggaaaag ttaagtgaaa ctgataaaaa
    18001 gttaggagaa acgagtaaaa aattagaaaa aacgaataag gaattaaatg aggttaaaaa
    18061 taaagaacca gaaagagaga taactgtagc tgatgaggaa gatttatatt atcaagctct
    18121 aaatttaaag aaaaacaaag aatatgaaaa agccatagat aattttaaaa gtgtagtttc
    18181 ttctggtaaa actaaaaaat atatttctga gtcaatatat caattagcaa taacaaataa
    18241 attattagga aataaggatg aagctataaa atattacaaa aaatatataa atacttatac
    18301 taaaaatgac cagtattatg atgattcata ttacgagttg ggtatgcttt attatgataa
    18361 tggcgattta aaaaatgcac aacagacttt ttatagtttg agaagtgaag ttccagacag
    18421 tatgtataac aattcaaaaa taaaagaaat tttaagtgaa aaataggttt atatagttaa
    18481 gttttttgaa acaaccaagt atgtatttta ttaaattaaa ataaaaataa atttaaaaag
    18541 agattgtgtc aaattataac tttctatttt aaagaaagat atttgcttgt tgataaaact
    18601 acaaatatat gatttgaagc aatctcttta atttttggag gtggaaaatg aaagttttca
    18661 atcaattggt aataatttta tctatctggg taataggaga gtatataagt tcctttatac
    18721 aaggaattat acttattcct gggagtatag ttggtatgct attattattt ctactactac
    18781 aatttaaagt tttagacttg agtagtatag aaaatgtaag tggatttttc ctcgataata
    18841 tggctatatt ttttattcca gcaggtgtat cacttataaa gtcattagac ctaataaggg
    18901 aaaatgtatt agtgctttta ttagtcatat gtttaagtac actaatagta atgtatacta
    18961 ctggaattat tgtggagaaa atgataaaaa agaaggaaga aggacaaaaa aatgtataat
    19021 gtattacaga caccagtatt tggtataata gtaacaatag tttttttcaa tttaggaaga
    19081 tacattcaac gaaaaacaag taatcctata tgtaaccctt tattaattgc aatcgtagga
    19141 ataatattgt ttttaagcat atctaaaatc ccttatgaga attacaagat aggagctgat
    19201 agtctaaatt tctttttagg tcctgtcaca gtagtacttg cagttccact ttataaacaa
    19261 tttgaattat ttaaaaagca tatgtttgag attattgtag gaataggttg tggtatagtg
    19321 atttcttttg tatctgtatt gataattggc aaaatagcta attcagatgt aagtataata
    19381 aattcactaa ttccaaaatc tataacaaca ccaatgggaa tatctttagc aaattcttta
    19441 aatggtgtag aatcaataac agttgtagcc ataattttta caggtatttt tggtggtatg
    19501 gttgcttcta cagtattcaa actggggaat ataaatcatc cagttgctaa aggaattgct
    19561 cttggaacat ctgcacatgc tttaggaact acaaaggctt ttgaacttgg agaaatagaa
    19621 ggagctatga gtggtgtttc tattggagtc tctggtacaa ttacagtaat actaattcca
    19681 attataatga attttatata aaaaataaaa ttaagatgag taccttgaat acttaaaaca
    19741 aaataaaata aaaaaaattt aaaataaaaa ctgaacgaaa aaaatgcaaa taatgaaata
    19801 aaaaaatcat gtactgaaat tataaaaaga aaaatttcat ttaggaatta attataaaga
    19861 gacaattact aaacttgaat ttgctaaaat aaaataaatt actgctaaaa tagatattta
    19921 gaaaaaaata ctataaattt actatattgc aagttaaata ataagcagta tcatattatg
    19981 ctagaaaaag tttcatttaa ggcataaatg agatgatttg aacgttttta aagattagaa
    20041 tataaaagat tgcactagac atatatgaaa aagataatat aatagatgat gtaatagatg
    20101 acagttcaag aaattataaa aaaaatttat ttgaaatatt atctattaca tcaggaataa
    20161 tatatgtaag agtaagcttt taaaattcta aagatttaga aagattgaag ggagaaagaa
    20221 atgagtgaag ttaagaatac cctacaaaaa catccagttt taagatattc aacaatgcta
    20281 tttgggatgt tgatgacatc tataggaata aatgggttct taagaccagc acatctgctt
    20341 agtggaggtg ctacaggtat agcaacatct ataaactatt tgacaaatat aaatgtaggt
    20401 ttattaacat ttttaatcaa tattccaata tttatcttag ggttcatata ccttgaaaaa
    20461 gagttttgta tttcaagttt ggtaaatatg attgtatttt cattattatt aggagcaact
    20521 caggaaataa gtaatattat acctatacat gatatattgc ttcaaagtgt atatggtggt
    20581 atattatctg gtttaggagt cggagttgta tttagaacta ggtcttctca aggtggtaca
    20641 gatataatag ctgcaatact aaaaattaag aagaatatag aaatgaaaga cactgcttta
    20701 gcaataaatg gcttgatagt acttacagga agttttttat ttgggttgga tttagcatta
    20761 tacactctta taggtctatt tctaaatgcg tattcaatga gttttattaa agatgcaatg
    20821 aattaccaaa aatctgttat ggtaatgtca aatgaagtcg atttaatagc agaagatata
    20881 atgaagagtt tagttagagg tgtaactttt ttagatgcag aaggtgcata cactcatcaa
    20941 aaaaagaaga ttatatatac aatagtttca tcaaatgaga taccaaagat aaaagatata
    21001 gctttgaagt atgataaaaa ggcctttata tctgtaaatg atgttacaga agtaaaggga
    21061 agagggttta aagcaaaaga tttatagaaa attttacaag ctgggagttt ataatgtaaa
    21121 tttgtatatt atatctctca gcttatttaa atttagtata cgaaaataaa ggttttatta
    21181 atgatttgtg tatcacttta aaagatatca ttttaaaatt aaaattgaaa aaattactta
    21241 atatgtagaa aacatgacca ctattaggat ataataaatt taatagattt agtaaaatac
    21301 ctaggaataa cgttggagga atggcattat gaatttatct aaagaatatg ttaatttgta
    21361 tgataattat ttgaaatata taaatgaagt aaacaaagag attagaagta taaagaacat
    21421 gaagaaacgt gttttaaaaa ggggcgattt tgctccggaa aatgaagtat tagaattgga
    21481 aggaaaaaca atacgcgata tagatgatgt cgatacaaaa aaaccaaaaa ataaaatcta
    21541 taaattttca aatggggatg tatatgtagg gaaattctta gagggaaaga tggaggggaa
    21601 aggctcatat actttttttg tagatgaagg aacagttatg gagtacatag gagaatttaa
    21661 agaagataaa aaaaatggaa aaggaaactt tatattttca aatggaaatg aatatatagg
    21721 ttattttgag gaagatatga tgagcggtat tggaaatatg ttatatagta gtaaagatga
    21781 atatattgga acttggaaaa acggaaaaaa agatggaaaa ggtatttata agtggagtga
    21841 tggatgtata tatgcaggtg aatttaaagg tggaaagatg gaagggtacg gtatttgcta
    21901 taatagtaaa ggggaagtac tttatgaagg agaatggaag aataatttga ttcatggtaa
    21961 gggaacttat atatgggaaa aaggtaaaaa gtatataggt gaatttatgc atggtaaaaa
    22021 acatgggcaa ggaacttttt atctaaataa tgaattggtt tatgaaggga cttggaaatt
    22081 tgataagcca tctatatttg atagaacatt agatgaaata ttttccttga gactgtagct
    22141 ttatctaatt agtagataaa gcttttttct aatacttact gtaaataggt atgaatgata
    22201 ttgattagta tacactatca atattaactt aataaatgca atattaaaaa attttgattg
    22261 attttaagaa ggagagatat attgaagaga ttaattgctt tagtgttagt agtacttatt
    22321 gttttatttt atagtcctga attattaaaa gaaggatatg atttaaaaga aaagtttttt
    22381 caaagtgaaa tatggaatga atttttagac aaaataaata taaatgataa ttctaagaaa
    22441 gataaaaaag aaaaaagtca aaatttacaa tcaaataata ctgaagggga gaacttagga
    22501 gagtcagata taaagaattt tgaaaaaata agcttaggag ataatttaag ctatgtttta
    22561 tcaagtatcg gcaaacctgg tagaattgat attagtgaat atggttttga ttggtatgta
    22621 tataatcaat atggaaaaga atttgcaatg gttggacttg aaaatgatga agtagtagca
    22681 ctttatagta attctattaa ttcatgtgaa aatcaagata taaaactaaa tcaagataga
    22741 caaacagtga gaactaagat tacaccatta aaatataaga gaaaaggaaa taccagatat
    22801 ataataaact cagaaaatca atatgatata ataagtaagg aaggtaagta tattacaatt
    22861 ttctacgata ttcatgaaga aaatagagtt tgttcatatt taataattga taaaagcact
    22921 gaagatgagt ttaaaaattt atatccagat gatagcgaag aacttaaaaa atgttttgaa
    22981 cttgaagtta tagaccttgt aaatagtgtt agaaatcaaa gagggttaaa ctcattgaga
    23041 tacagtgaac aggcaacttt gagttcaaga aaacatagtg aagatatgag agataataat
    23101 ttttttgacc atgtaaataa gaagaatgaa actccatttg acagaatgaa aagagaaggt
    23161 atagtatata cttcagctgg agaaaacata gcagcaggac agattaatgc aatatatgcg
    23221 catgaagctt ggatgaactc tgaaggacat agaaaaaata ttttggggaa ctataataat
    23281 attggagttg gagtaatatt tggaggaagc tataaaacat attacactca aaatttttat
    23341 aaataactaa aatatttgaa aggaagttaa aaaatgacat catggaaaaa aaagacagta
    23401 tataagtgct taatagcagt agccttgttt tgtggaatag tgttaatatc taattttagt
    23461 aaagtttctg ctttgatgat ggatactaat ggaaatgttt tgattaaaca tggttcaagg
    23521 gaaaaaaagc taatagcaat tacctttgat gatggtcctc atcctaaaga aactagtcaa
    23581 gttttagatg tattaaagaa atacaatgta aaagctactt tttttatagc tggaaaacat
    23641 gcaaaatggt ataaagaacc attagtaaga gcaagtaaag aaggtcacga gataggaaat
    23701 catactttca atcatcctga tataagtaat ttaagttcta gtcagataga agaggagatt
    23761 gttaagtgtg aagacatttt gaaggaagta actgggaaaa aacctacatt atttagacca
    23821 ccattcggaa gttatagaga aaaagactta atagaaattg caaaaaagca tgactataaa
    23881 gttgttttat ggacaggtgt agatgtgaaa gattggaaaa atccaggagc taatagcatt
    23941 gcagataaaa taattaataa agtacaaaat ggagatataa tacttttgca tgattatgca
    24001 actaatgata cagttgaagc tttagatatg tttattccaa aaatgattga aaaaggtttt
    24061 aaatttgtaa cagtttctga gttgataaaa taatttttag gggggagatt tacagtatga
    24121 ttcttatgat agacaattat gattcttttg tatacaacct agtccaatat atagaagaat
    24181 taggggaagc agttgtagta aaaagaaata atgaaatcaa aataagtgac attgaagaac
    24241 taaatccaga ggtaattgtg ttatctccag gaccgtgttc tccaaaagaa gctggtattt
    24301 gcatagatat tgttgagcat tttaagggga aaaaacctat actaggaatt tgtttaggtc
    24361 accaaactat tggacatgtt tttggaggag atattataaa agcacaacag cctgtgcatg
    24421 gaaaagtata tagtatcaac catactaata aaggagtttt taggggactt aaaaatcctt
    24481 taaatgtcac taggtatcat tcactaatag ttgactccaa tacagttcca aaagaattag
    24541 aaattacagc aattactgat aaaggggaaa taatgggaat taggcataag aaatatttaa
    24601 tagaaggagt acagtttcat ccagaagcta ttttgtcaga gtatggtcat gaaatgctta
    24661 aaaattttat aacagaagct agagaaaggg tacatatgta atatgataag agagataaat
    24721 actaaattaa attcttttga gatatttact atttttagaa atgaacatga cagttttata
    24781 ttagatagtg ctatggataa agaaaaattg ggaagatatt cttttataag tagccaacca
    24841 tttaaggtat taaagtataa agatactgat gaaaatcctt tagaagtttt aaaagaagaa
    24901 ctacataagt atagggttgt aaatgatact aatcttccat ttgtaggagg tgctgtggga
    24961 tatttatctt atgatttagg taattacata gaaaatttac ctagaactgc tgtagatgat
    25021 attgagatgc ctgatatgta ttttggattt tataatcatg taatagttat agaccatctt
    25081 gtgcaaaaaa cttatatagc tactccaaac atagatatcg aattagaaga aaagataata
    25141 gatgatatag aacaaagaat attaaaagaa gagaaaaaag gtatagatag catatgttat
    25201 gaagaaaaag aagtaacacc tataagactt aaatctaatt ttacaaagga agaatttaaa
    25261 aatgcagttc aaagtgttag agagtacata agacaaggag atatatatca agctaattta
    25321 acacaaaggt tcagtggaga aacagaactt acaagctttg aattgtatag ggacttaaga
    25381 agatttagcc cagcaccatt tggggcattc ttaaactttg aagatgcaca tatattatca
    25441 aattctccag aaagatttat aagatgtgtt aataagagaa tagaaactag accaataaaa
    25501 ggtactcgtc caagagggaa agataaagaa gaagatttaa gacttcaaca agaacttaga
    25561 aatagtgaaa aagatagagc tgaactactt atgatagtag atttagaaag aaatgatatt
    25621 gggagaatat ctaaaactgg aagtgttaaa gttccagagc tatttgtaat tgaaccatat
    25681 gcaaatgtaa atcacttagt ttccacagtt gtaggtgagt taaaagatga taaagatgct
    25741 actgatgtaa taaaagcaac ttttccaggg gggtctataa caggagcacc taagattaga
    25801 gcaatggaaa ttatagacga attagagcca acgcaaagaa atgtatatac tggttcaata
    25861 ggttatatag gttttaatgg tgatatggat tttaatatag caattagaac aataatcaaa
    25921 aatgacaaaa aagtctactt tcaagttgga ggaggaatga cttgggattc tgacccagat
    25981 gaagaatatc aagaaacact tgataaagct aaatccataa tgaaggcttt gaggggatat
    26041 tatgaagaat aatgtaggtt ttgatagtag tttaagtaaa tttggaattg gtttgtttga
    26101 aactattaga gttaaaaatg gaatagcagt agatttagat atccatatag aaagaatgct
    26161 aagctcaata aattgtttgg atttagatat aaactataaa aaagatttct tgataaatga
    26221 aattgttaca tatataaaaa aagaaaatgt cattaataaa gcacttagaa ttactgtttt
    26281 tgatgaaggt tataatatat ctattaggga tattccatat aatcaagaga cgtatgagaa
    26341 ggggtttaaa ttagctatat ctccaatagt tagggggaat agtttaattc atagacataa
    26401 gactactaat tattttgaaa acatatatac caaaaatatc gcaaataaga ctggttataa
    26461 tgatggtata tttttaaatt cagaaggtgt aatattagaa tgttctatga gcaatatttt
    26521 ctttataaaa ggagaaagag tgtacactcc tagtgataga ttaccgatat taaatggaat
    26581 aataaaaaaa aggattatag agatttgtga cgaattacat atagagttga tagaaaatga
    26641 aattaatata tcagaaatta gtagttttga ttttgttttt gtcacaaata gtcttatggg
    26701 cgctataaag gttacagaaa ttgacaaaat aaaatttaat aaagaaaatg ttgtttttga
    26761 taaaatagtg aagttattag aataaattaa aatattaaca atttgttgga gacaaaagat
    26821 gataggttgt atttatgaag tatacaatga agagatgact aaaataatag aaaaccaaaa
    26881 cactttggct gtatatttag tgggttctag taaagatgta gattttactc tttatgatgt
    26941 agtgataaac gatattgatg tgttcgtttt tgtaaaagaa ggggaaaaac aagagagaat
    27001 actgtttaaa aaaaagggaa tagaatttga tgtaaattac ttttcaaaag atggtgtaaa
    27061 aaaattatta tcaaataggg aatatttctt tgtgaaagaa atgaaagatg ctaaagtact
    27121 gtttgatagg gagaatatat cacatattat aaaagatata agtagagata tttacttaga
    27181 aggacctagt aaaatgtcat tagaggaaaa gtcttttata aagcaagata ttggagcaaa
    27241 aatattgaat ttaaaaaata aagaaaaatt tgatgttttt gaatatcatt ttttgacgaa
    27301 tttatattta aaagatataa taattgggta ctttaatata aatgataaat gggtaccaaa
    27361 agacaaaaaa ttgctaaaag ttttaaaaaa agaaaataat gagctttttg aactagtgtc
    27421 aaaagtaagt gaaaattatg actatcaaag acttttagat gtttataatt atatttttaa
    27481 agaaattgag acaaaagagg ttataaaact aatcttctag tcacaaatca atggggggag
    27541 tttaaatgaa taaagttgat aaagaaaaga tacaacatgc agtaagagaa atactagagg
    27601 caattgggga agacccagat agagaaggtc taatcgaaac tccaaataga gttgctagga
    27661 tgtatgaaga gatattctct ggtcttagtg aagaaccaag agaccattta aaagttcttt
    27721 ttgcagatga aaaacatgaa gagttagtat tggtaaaaga tattccattt tattcatgtt
    27781 gtgagcatca tctagttcct ttctttggaa aagctcatat tgcttattta ccaaaaggag
    27841 gaagacttac agggctttct aaacttgcta gagtaataga tacattggca aaaagaccac
    27901 aattacaaga aagaatcact aaaaatgctg cagatataat aatggaagaa ttacaacctt
    27961 atggtgtctt agtagtagta gaagctgaac atatgtgcat gacaatgaga ggcgtaaaaa
    28021 aaccaggttc aaaaactgtt acatcagcag tgagaggaat atttgaaaag gacattgcgt
    28081 ctagagccga ggctatgagt ttgattacta tgaagtagta atttattata gatataatca
    28141 tttattgaga taaaatttaa tttaagggag tgtcattaat tgtgtttgat tatggtaaaa
    28201 gaacttatat aatgggaata ttaaatgtaa ctccagatag tttttctgat ggaggagatt
    28261 ttaataactt ggacatagca atacaacatg caaaagatat ggttgaccaa ggtgcagata
    28321 taatagacct aggaggagag tctacacgcc caggtcatag ctatgtagac tcagacgaag
    28381 agctaagaag agtaatccct gtaataaaaa aacttaaaca ggaattggat ataccaatat
    28441 caatagacac ttataaggca gatgttgcag aagaagcatt aaaattaggt gtcactatgg
    28501 taaatgatgt ttggggtctt agaaaagata aaaatatggc ttctgtaata ggaaaatatg
    28561 atgcagaagt gtgtataatg cataatcaag atgggacaaa ctatgacaaa gatataatgg
    28621 agtcaataaa agactttttt aaggtaagta tagaaatggc aatgagttgt ggtgtaaaaa
    28681 aagaaaaaat agtacttgac ccaggtgtag gatttggcaa ggattttgaa caaaatattg
    28741 aagtattgag aagattaaat gaattaaaag atttagggta tcctattttg cttgggacat
    28801 ccagaaaatc agtaatagga aaagtacttc cagttgagcc taaaaaaaga ttagaaggta
    28861 caatagctac tactgttctt ggtataagag atggcgtaga tatagttaga gtacatgatg
    28921 tttatgaaaa tttaatggca gctagaatga ctgatgcaat atatagaaag taggtataat
    28981 ataatggata aaatattact tagtaatcta ggattctatg gatatcatgg tgttcttaaa
    29041 gaagaaaatt ttctaggtca gaaattcttt gtcgacatgg aactttatat tgattcaaga
    29101 gaagcaggtc ttagtgacga tataaataaa tctgtaagct atgccgaggt ttacaatgtt
    29161 gttaaagata ttactgaaaa taaacaattt aatcttttgg aggctttagc ggaaaatatt
    29221 gctgaagaag tcttgaataa atttatactt ataaatggag ttatggtcag agttagaaag
    29281 cctgaggctc ctgtaaatgg tatttatgac tattttggtg ttgagataag gagaacaaga
    29341 gatgaataag gcttatttgg gaataggtac taacatgggt gatagatttg ataatttatc
    29401 aagagcatgt gaacttttga aaaatagtga ttctatatat aaagtaaaag aatcaagctt
    29461 atacgaaaca aaaccatggg gatatacaga acaagcagat tttctaaata tgtgtgtaga
    29521 aatagaaaca gagtttgaac cttatgagtt gttagaatat tgtcaagaaa tagagagaga
    29581 actacacaga gagagaatag ttcattgggg accaagaact atagatgtag acgtactatt
    29641 ttttaatgat gtagtttcca ctgatgaaag actaacaata cctcatccaa ggattcaaga
    29701 tagagctttc gttttgatac cacttatgga tttaaatgaa gaacttataa taaatgaaaa
    29761 aacaataaaa gaacatttaa acctcctatc tgctgaagaa agagaagaag taaaggagct
    29821 tgtaggttat gaaagaaaac ctatttaata ataattcaaa gatagagata aatacaaata
    29881 acaaaaagat aactgtaaaa aaagataaac ttataatagc tggaccatgt gcaatagaga
    29941 gttatgagca acttttggag actgctaaat ttgtaaaaag tcagggagca aatattttaa
    30001 gaggtggagc atacaagcca agaacatctc caaattcatt tcaaggatta aaaaaagaag
    30061 gtcttgaaat attaaaagcg gttaaagacg aagttggtat ggctgttatt acagaactta
    30121 tggatgtaag agatatggac gaactgtata gtataagtga tataattcaa attgggtcaa
    30181 gaaatatgca aaactttact cttttaagtg aagtgggaaa acaaaataaa cctgttatgt
    30241 taaaaagagg aatagcttcc acaattactg aatggatagg tgcttctgaa tatatagcaa
    30301 tagaaggaaa tagtaatatt ataatgtgtg agagaggtat aagaacgtat aatgattata
    30361 ctagaaatac attagattta gctgcagtac ccattattca aaaggaaaca ggtcttcctg
    30421 ttgtggtaga cccaagccat gccactggag ttagatattt ggtaaaacct atgtcactgg
    30481 catcttttgc atgtggagca gatggaataa tggtagaagt acatcctgac ccagaaaatg
    30541 ctttgtcaga tggtgctcaa tctctttgct ttaatgaatt tgaagattta atgaaatcaa
    30601 ttaataatta ttaaaagagg caagagcctc ttttttattc gtaatcgttg ctaaggtaaa
    30661 tttataagtt ataatttgat agttagatta tttaatataa ataaagattt aatattgata
    30721 gaattaatat aaaaaataaa aattttttga ttaataagaa ggatatcaat aaaaaaagtc
    30781 gaattgtgta tattgagtga ttgacaaatt tagaatatag tgtagaatag aaatgttaac
    30841 acaaaaaatt acaccagaag gtgataatat gaatgatata aaagacatta ttgaagaaat
    30901 aaaagctaga tgcgatatag caagtataat atctgaatat atgaatataa aacaatctgg
    30961 agctaattac aaaggattat gtccatttca tggcgaaaag acaccttctt tttatataaa
    31021 tacgtcaaaa cagatttata agtgttttgg ttgtggtgaa ggtggagacg taataaattt
    31081 tgtcatgaaa atggaaaatt tggattttat ggatgctgta aaaattttag caaataaatg
    31141 cggtatagaa attaacacta acatgaatga agaaactagg attaaaatag aaaaatctaa
    31201 gaaatttcaa gatattcata cggaagcagc tagattttat ctttcaaact tattgggaag
    31261 caaaaatctt gggtatgaat atttaagaat aagaggacta gatgataaaa ttattaagaa
    31321 atttggttta ggattttctt tggattcatg gaattcactt atgaatactc taatcagtaa
    31381 aggatataaa aaacaagact tattggagtg cggtctaatt gcaaaaaata gagatggaac
    31441 taattgctat gataaattta gaaatagagt tatgtttcct atattcgatt atagaggaaa
    31501 tataatagga tttgggggaa gagtattaga cgattctttg ccaaaatact taaattctcc
    31561 agacacgtta atttttaata aaaaacagaa cttatacgga ttaaattttg ctagaaaaaa
    31621 tttagaaagt aaaactatag tcttagttga aggatatatg gatttaattt cgctttatca
    31681 gtatggtata aaaaatgtag tagcaaccct tggaacagcc ttgactgaac aacaaggcat
    31741 tttaataaag agatatgcgg acactgctat tatatcttat gattctgatg aagcaggaat
    31801 aaaagcgact ttaagagcta tagatatact tactaaatta ggtatcaatg taaaagtgtt
    31861 agacttaaaa gatgctaaag acccagatga atttgtaaga aaatacggac ttagtgatta
    31921 taaaaaagct atggatgttt ctactcacta cataaaatat aaaatagatc atcttaaaaa
    31981 agaatttaac attcaaaaag atgaagaacg agtgaagttc gcaaaagaag cttctaaaat
    32041 tataaaacaa ttgacaagtc ctgttgaaat tgatttttat acaaagtatt taagcaatca
    32101 aatagatatt aatgtcgagt ctataaagag agaagtttat ggtaaaaatt acaataagcc
    32161 atataataac aaaaatcaaa agaaaataga agaaaaagta attgaaaaag tcgaagtcag
    32221 acaagacgga aaacaattag ttgaagaaac tctaataaaa ataatgttag aagacaaaaa
    32281 aattagagaa atagcgttac ttaaagtaga agaaagtgat tttttattga aagaaagtaa
    32341 agaaatttta aattacatga ttaaaaatca agaattggac aaaataacta ttgacaaatt
    32401 gaaaagttta aacatatccg aagaatactt aaaagaatta aattcaattt ctttgaatag
    32461 tataaactta gagaacacaa aagaaataga aggaataata acaaatatca gaaaaaactc
    32521 attagaagag caaattaata gcttgttgag agaacaacaa gaattagaaa ataataatga
    32581 tatgaaagag gtagatggta gagttatgga aattgcttta aaaatagttg agataaataa
    32641 aattttaaag agcttgtaga gaaaggaggt ttgagtgtgg aaaataaatc gaataaaaaa
    32701 gagttaaaaa aggttactgc taagacatta atagaaaaag gaaaaaagca aggttctctg
    32761 acacttgcag agataatgga agctttttcg gagactgaac ttgataagga tcaagtagaa
    32821 aatctttatg agactttagg aaatttggga atagaaataa cagaaacaaa aaactataaa
    32881 gctgatatag atttttcggt tgctgatgac gatttaagta taggccactt agatgaagat
    32941 gcagaggcaa tttcacatga tgactcttct gcaatagaaa tagaaactgt ggatttatct
    33001 ttaccaaaag ggataagtat agatgaccct gtaagaatgt acttaaaaga aataggaaaa
    33061 attcctctac ttaaaccaca tgaagaagta gaatttgcta gaaggatgca cgaaggtgat
    33121 gagatagcaa aacaaaggtt agttgaagca aacttaagac tagttgtaag tatagcaaaa
    33181 agatatgtag gaagaggtat gcttttcttg gatttaatac aagagggaaa tttaggtctt
    33241 ataaaagcag tcgaaaaatt tgactataca aaaggatata agtttagtac ttatgcaaca
    33301 tggtggataa gacaagctat aacacgtgct attgcagacc aagctagaac tataagaata
    33361 ccagttcata tggtagagac tataaataag ctaataagag tatcaagaca actacttcaa
    33421 gaacttggaa gagatccaaa accagaagaa attgcaaaag aaatggaaat gacagaagat
    33481 aaagtaagag aaataatgaa aatagctcaa gaccctgtgt ctttagaaac accaatagga
    33541 gaagaagaag atagccattt aggtgacttt attccagatg atgatgctcc agccccagca
    33601 gaggcagcag catattcttt attaaaagaa caaatagaag atgtacttgg ttcattaaat
    33661 gatagagaac aaaaagtatt aaagcttaga tttggtcttg aggatggtag agccagaact
    33721 cttgaggaag ttggaaaaga gtttgatgta actagagaga gaataagaca gatagaagca
    33781 aaagcactaa gaaaactaag acatccaagt agatcaaaaa aacttagaga ttatttagac
    33841 taaaaagata catcattgat gtttttgtga taaatgccat gagataagtg atatctcatg
    33901 gctattttgt agtaaagatt taattttatt tttgtagtta tttcagtcta atatctataa
    33961 cttggattaa atcctataaa taaagggaat tgaaaggaga attatatttg aaattaacag
    34021 atagattatt gaaaatagca tcattagtgt cagatggtaa aaaaatagct gatataggga
    34081 cggaccacgg atacatacca gtatatttgt taaaagaggg aaaagtacca tttgcagtgc
    34141 tagcggatgt taataaagga cctcttgaca atgcacataa agaagtcata cagaacaatc
    34201 ttttagataa agttgattta cgattgggtt ctggtataga gatacttgaa ataggggaag
    34261 tagaagaagt gataattgct ggtatgggtg gaatcttgat aagtgagctt ctcgaggcta
    34321 aaaaagaagt agcacataat gtagaaaaat taatattaca gccaatgcaa gcacaagaag
    34381 aacttagata ttacctttta aacaacggat atgaaatttt agaagaagtt ttagtgagag
    34441 aagattttag aatatatgag ataattgttg ccaaatatac aggtaaaaat actataatag
    34501 aagatgagat atattatgag gttggaataa agcttttaga aaacaaagat tctcttttta
    34561 atgattttat agaaaaaaag ataaagacat atagttctat agttaataaa ttagaaggga
    34621 aaaatgggga agctatagat aagaagagaa aggaaagcga agttgcaata aaaaaacttg
    34681 aaaatttaat aaaataaata ggaggtatat atgctattaa agagtctaac tagaaaaatt
    34741 gaaaaaaagt atcctctaaa cttagcagag gattgggata atgtaggtct tatagttgga
    34801 gattttgata tggatgtaaa aaaggtattg gtatctttag aagctaatga agatgttatt
    34861 gatgaagcta tatccaaaaa tatagattta attgtgacac atcatccttt tatatttggg
    34921 aaaattaata aaattaatag tggagattta aaaggcagac ttatccaaaa acttattaaa
    34981 aatgatatat cactttattc tatgcatacg aattttgata tagcatttga tggtctaaat
    35041 gactatttta tggaaattat ggaatttggt aattcaaagg ttttagatat aactaaaagc
    35101 gaaaatttgt ataaattagc agtatacgtt ccacataact attcagatga acttagaaag
    35161 gttttaagta attcaggagc aggtcatata gggaattata gtgattgtac gttttcaata
    35221 gaaggtgaag gacaatttaa gccattagaa ggctctaatc cgtttcttgg aagtgtaaat
    35281 gatatagaaa ctgtaaatga agttaaaatt gaaactgtag taccacagaa gcttttaggt
    35341 ggggtaataa gttctatgtt agatgcacat ccttatgaag aagttgcata tgatttgtac
    35401 aagttagaaa ataaaggtga gatatttgga ctaggtagaa taagtaaact tgataaaagt
    35461 atgacattag aaagtctttc aaggaaaata aaagagaaat taaatatgaa acacataaga
    35521 gtagttggaa accttagtac agacataacg aaagtagccg ttgtaacagg agctggttca
    35581 gaatttgtaa aaaaagctaa gagacaaggt gcagaagtac taattacggg tgatgtaaag
    35641 tatcatgagg cacaagatgc acttgatatt ggaatgtgta tagtggattg tggtcatttt
    35701 gatacagaag atatattcaa aaatgttatg aagagatttt tagatgaatt tagtgaaatt
    35761 gaagtaataa aaagtaatgt atatttgaat ccatttagta ctatttagca gtgtttgata
    35821 ataaaaatac tatataaaaa ataaatttga gtttaattag ggggataatg atgaaagtag
    35881 cagtaatttt tcatagtgtt tgtgggagta catatttatt agctagagaa tataaagaag
    35941 cgcttgaaga aatgaatatc gaggtagata tatttagagt aagtgacgaa gttgcaaaaa
    36001 cacttcctca atattatttg ataaattcca aagaatacaa agacgaattt gaaagtataa
    36061 atgtaattaa atctggaaaa gaaatattag attatgatgc tatatttatg ggttcaccaa
    36121 catattatgg aaatgtatct ggaccaatga agatgtttat ggatagtttt tctgatattt
    36181 gggtaggtac tcctttgagt ggtaaaatat ttggttgttt tgctacagca ggttcacaac
    36241 atggaggagg agaattagct ttgcaagcta tgaatatatt tgcccaacat atgggaatga
    36301 ctcttttgtc agttccatgt tcagtaagag gaggttatcc agcgtatgga atacttcata
    36361 tagcaggaga taattctgat ataagaccaa atgatgatgc caagataggt ataagagatt
    36421 acctaaaaag acttaacata taagaatatc atataaaaat acctaaaagc gactgtatat
    36481 acttttaggt atttttactt aattataaac gtgaagcttt accagaaata tggtcaattt
    36541 ctattttaac aacagttgtt aagttcatat ccttctctat gtacttcatg ccttcttcaa
    36601 agaatccttt agaatatttt tttattattt ctacaagagc atttttcttt tcttcatttt
    36661 ctacagtgca agcttttcca aaaactatag cactagaata agtagtacta aatttatctg
    36721 gaataacact ttcattagca actactaaaa aagaaacttt gttgtttttc gatatattat
    36781 ctaacttatg cccatttctt gcacaatgaa agtaaataga atcattaaag tatacataat
    36841 ttacagcaac tccataagga taaccatttt cagatatagt ggaaaaagta ccaaattcac
    36901 cattttttaa tacttcaaca gtatcttctt ttgtcatttc tctttttttc aatctcattt
    36961 ctttaaacat aatatcatcc tttctaattt tgatgcttaa attaaatttt agaacattta
    37021 taaagattat gctatgtttt ttacaaattt taagaaaaaa atatagttta agataaaagg
    37081 tagtgaaaga gaagctattt ggaggtttat ttatgatatt attgttagag tagtataaac
    37141 aatactaata aatttaatag ctggtttctt gttatttaag atagtaagca tgtctataag
    37201 aaggggaagt tatcgagggt agaagttgtt atttataact atattataaa gaaaatatta
    37261 ttatataaaa taataaatag taaagaggta tcaagttgaa tataatactt aatacctctt
    37321 caaaattaag ttataaaagt gttgtgggaa ttgtcctata catggtaagg agataataaa
    37381 gaatgtatct ttaaagtctc aactaaacag caaaatttat ccaacaaaca tacaataaat
    37441 gcttctttgt atctaggtgg cttaacagta agtggccaca tgtgttttaa aattatgtcc
    37501 gtttcttttt catttatttg aaaaaataaa gttgcatttt ttaatgcttc tctagggtga
    37561 gtaaatccat gtaatccttt tctatcacct ttgtaatgcc aatcataaag aaaaaagtcg
    37621 tgtagtaaag ctcctctaat aacactttga atatcaacac ttaagtgcaa ttttttacat
    37681 aatagataac tataatatgc tactgacaaa ctatgttcaa gacatgaaac attgccatga
    37741 tgaggaaatg ttctcataag ttgaactttt tcagaagaaa gtatctcaga agcacattca
    37801 ttaaaatatt tgatttcagc ttttgacaac attatgaaaa tctccattct tagtaatata
    37861 taaatactat acttcaacga taatatgaaa tatttcttat cctacaaaat ataataccca
    37921 ataatacaag taagatacct tttatggtat tttaaagtaa gagagatttt attaggctaa
    37981 acaatgaaaa aattaaaagt aaagctatag caatatatag tatttttcca agaggattgc
    38041 caaaatttat agtccatcca ataccaaacc ttttattaac cattaatgat gggtcatttg
    38101 gattattata catgatgcct gctatccaat acttctcatc ttcttctgga gaataagatg
    38161 agttgaattt taaatttggg gaattaatat atgttgctat taaataaatt ataactaaaa
    38221 gcgttgtaat attcatagca gttgatagtt ttgtttttat tgataaaaga gtagtaaaaa
    38281 actgtgttgt cattataatc atcaatatta aaaacaaata accaatttta tttaaatatt
    38341 taatgttttc aactctactt tcttcaattc tatttgtatc tatcttaatc cttgacttta
    38401 tggatccaat ggaagttata tataataaaa ccattaaaca aaagtcagta ccgattaatt
    38461 taaatacatt aagaaatgat ttatctataa aaacatcagg agacccattt atgttccaat
    38521 gagtggcaat tgagtctggc aattgattat aattaaatgc tataaaaata ctcattccca
    38581 aagtaaatag tatcggtatt aaatagatta ttttaaattt ttttattatt ttatttttct
    38641 tatttataaa atccatatct actattaatt ttgaatctat atctatatga cctaatttag
    38701 aataaatttc tgatttaagc ttcttagctt ttttatgtgt atgaatatat agtatacttt
    38761 catataataa aaaacctata ataccaataa aataggaaag ttccaattta ttaaatgtaa
    38821 agaccagaat aaaaactaat ataaagtcta taataaaacc taaagataat agttttttaa
    38881 attctttatc taactttata aagtaattat aatttttatg ctcttggtct atagaaactc
    38941 catagatgtg ttttttacct acaaaatagg gcataaaata aaatgtagca tacatcaaaa
    39001 ttagaactgg aaatattgta aatagacatt caatcttact cataatattt cacctcatac
    39061 ttgtcaaata aacttgaact tagtgacaaa aattcttctt tactaatacc tgataaataa
    39121 gattttgcca ccaataattc caagtcaagt ttattttttt gacgagttgt attatctatt
    39181 ttcattggta gttgagctac aatagcacct tttcttctat caatatttaa gaatccctct
    39241 tcttttaatt cattgtagga tttgttcact gtatgtaaat ttacaccaat attttcagcc
    39301 atacttctta cagatggaag tgattcattt attttcaatt ctcctgaagc tatagcctta
    39361 gttatttctt ctttaatttg tacataaata gatttatcac tattaaaatc taaatttaaa
    39421 accataaaaa acctccatta tgctttataa atgaaaacga ttgttatata catagtataa
    39481 cataaaaaat aataacaaac acaaaatata agtttatatt ttgtaaaaat tgtaatttaa
    39541 aaaataacat aatttttatt aaaataatac agatactatt tatagataga aataaaaatt
    39601 agaaaggaga aaaaatatgt taacatattt aatgaaaaga caaatgcata aaaaagatga
    39661 tggaatgagt aaaccaatga tgattacagg agctgtaatt acaggtgtag gagcatatgc
    39721 tgtatataaa atggtaaaaa gccataaatc taattctcaa atggtcaata caaattatta
    39781 tggaaatagc gattatgatg attataaata tgacgaattt gattatgact gtgattgtag
    39841 agacacaaaa caagattatg aatatgaaga attaaaaaaa aaagtaaaag aatttaattc
    39901 aagaagaata aattgtgaaa attgtccaca tgtttctcaa gaagaaatga tgcaatatgt
    39961 aaatagtgtt aaagatgtaa aggaagagat tgatttacat gaagatgcta aaaaagataa
    40021 tatttacaaa gaagatgaat acaaaaaata tgaagaagaa taagtaatat tttgtataac
    40081 tgaacaaata gtaatgtcaa attatttaat atctagtttt aatatgtaag ttgtaactta
    40141 acttgaaaga atcagtatca ttctttagat agttaagtaa caacttacat tttattttta
    40201 taaattttaa tagttctttg tgactatttt gtaattttag aaatatttta gtatccaggg
    40261 taattgtgta taataaaact tgtatagata gatagtatga aattaagcag atagtttaaa
    40321 attaaaatga aaagaaagga aatagttatg ttaaagttgt tgatagttga agatgatagt
    40381 actatatctt ttggaataaa gtatgcttta gaacaagaag gatttagtat agatatatca
    40441 aaggatttat ctagtggaaa agaaatgatt tcttctaatg aatatagcat gatactgttg
    40501 gatgttacat tgccagatgg aactggttat gagctttgtc aatatataag aggattctct
    40561 caggttccta taatattctt gactgcttgt gatgaagaag taaatattgt aatgggtctc
    40621 gatataggtg gagatgatta tataacaaaa ccttttagaa ttagagaatt aatatccaga
    40681 ataaaagctg ttttaagaag aaaaggtaat acttcagaag aaaataagaa aatacttaaa
    40741 tttggagatt taagtatata tacattagaa gctagagtat ataaaaatga taaagaaata
    40801 tttctgacat cagttgaata taaacttctt ttaattctta tacagaataa aaatactgtt
    40861 ttaagtagaa ctcaaatatt ggaaaaactt tgggatgtaa cttatgattt tgtaaacgac
    40921 aatactctaa ctgtttatat aaaaaggctt agagaaaaaa taggtgatga tttatgtgaa
    40981 ccaatctaca tagaaactgt aagggggatt ggttataaat ggataggaag tgaaaatagt
    41041 gtttctatat aatccagaag tgaagaagtt tttaagtaag tatgttactt tgatgtttat
    41101 tgttatcata atatctattg gatttagcat aatcaacgtg tccttaacta aagagatggt
    41161 tgtaagaaat aatcaggcta taataggaac tttatcaagc aagtatccaa acttggaaag
    41221 tcagatagta gacataataa ctcaagggaa gtctatggag aacatagatt atggtcagaa
    41281 aatattaatt aagtataatt atgataaatc tattaggata aactctgagc ctattatttc
    41341 taaattagtt ttagagacta tcaaaataaa tataatatta gtatgtataa tatttatttt
    41401 gatatttgtg ttggttgtta gatattttaa gtcaatttac aatgatttat cagatatgac
    41461 taagtatgtt tactatagtt cagagggtaa gccatttgat atgaaaaaca agaatcaaga
    41521 agggcagata ggtcttctaa aaactgaact cttaaagatg acaacaattc ttaatgaaaa
    41581 ggttgaactt ttaaaaagag aaaagatatt tttaaacaat acaatttccg atatatcaca
    41641 tcaattaaaa actcctatga cttctttaat aatgttgaat gatttattat ataatgatgt
    41701 accatacgaa gtaaaaatcg attttttaaa taagataaaa aatcaattaa atagaatgga
    41761 ttggttgatt aaaagcatgc ttaaattatc aaaggtagaa gcaaaagtga taaattttaa
    41821 aaaagataag gtaaaattta gtgaactcat acatagagca atgctatcta tgaaaatacc
    41881 tatggagatt aaaaatcaaa aactgactat agagggaaat gataatgtat cttatattgg
    41941 tgatattgat tggtccgtgg aagcattagt gaatataatc aaaaattgtg tagaacatac
    42001 tcctgaattt ggaaatataa ttataaccta taaagaaaat ccattatttt ctgaactaat
    42061 aataaaagat gagggtgaag gtatagataa aaaagatata ccacatgtat ttaaacgatt
    42121 ctatagagga agaagtagtt caaaagaaga tagtgtagga ataggtcttg caatgtcaaa
    42181 atctataata gaaagccaaa atggcgatat atatgtaaac agcgaaaaag gtcagggtac
    42241 agaatttcat ataatatttc ataaaatgta tgatagtgac taatctgtaa tatttaattc
    42301 actttatagt aatagccaaa acctaatata tgaagtatag aatataaata ttggaggtag
    42361 ttatggaaat tttaagagta gaaaatctaa ctaaaagtta tggtaaaaat gaaacaaaag
    42421 ttgatgctat aaaaaatgta agtttatcag ttgaaaaagg aacgtttata gcaataacag
    42481 gtccaagtgg aagtggtaaa agtaccttat tacatttatt aggaggagtt gacagaccaa
    42541 ctagtggtaa ggtttatata aatgatgttg atatatataa tttaaaaact aaggatttgg
    42601 cgatatttag aagaagaaat ataggtctta tatatcaatt ttacaactta attccagtct
    42661 taaatgtgaa ggaaaacata ttgcttccag cagagcttga caatagagat attgatagag
    42721 aatattttga tgatttaatg aaaacattag gtctaattga tagagaaaag caccttccaa
    42781 accaattatc aggaggtcaa caacagagaa cctctatagg acgtgcatta attaatagac
    42841 cttctataat attagcagat gagccaacag gtaatttaga tagtaaaaat tcaaaggaaa
    42901 tattagaatt gttaaaattg tcagtaaaaa aatacaacca gacactaata atgataactc
    42961 atgacaaaaa tatggcactt caagctgata gaattataag catagaagat ggaatcataa
    43021 aaagtgatga ggtgatgtag atgaatttat atacattact aaccattaga tacttaaaac
    43081 aaaataaaag aagaaccatt gtaaccataa tcggtataat actttcaaca gctttaattt
    43141 gtggtatagg aaatatattt gaaagtttta tggattatca aatgagagaa actataaaga
    43201 atgatggaag tttccatgtt acattttatg atgtaaatag aaaaaatgtg gaatatgtaa
    43261 cgaaatcagc tgaaatagaa aaacatgcat ttaccaaaca acttggatat tctaaattag
    43321 aaaatagtga aaatgctata ttaagtataa agcaatatga taaaaatgca ataaatggat
    43381 ataaagtttc tataaaagaa ggtagatttc cagctaagga aggagaaatt gttctaagtg
    43441 aaagtattat taatcttata gatgataagt taaaaatagg agataagata actttaaaag
    43501 taggggatat gtttgactca agcaagaaaa agattgatag tatgacattt catgaaggtg
    43561 attatatagc aaatgaaaaa gatagaactt ttaaagttgt tggtataatt gaaaaacctg
    43621 ggtttgaacg ttataatgga attgcaacag ctatagccta ctttaatcct atgaataatt
    43681 acaatgatac aattaatgta tcagtagcag taagaaatcc taaagatgtg tataagataa
    43741 gtaataaaat aagtaaaaat atatattcaa ataaagataa agaagcagtt tctgatatga
    43801 taaaatacaa tgaacattta ttgaggttac aaggtgctag taagtattca aatataaata
    43861 actctataaa gtctataatt atagcagtaa ctattttagt aatcatatgt acaattgcta
    43921 cagtgtacaa ttcctttagt atttctataa atgaacgaaa aaaacagttt gggatattaa
    43981 attctatagg tgctacatct agtcaaatta aaagattagt ttttatagaa ggtattataa
    44041 ttagtttaat tggtatacca ataggactaa tatctggaac agtagctata gacctattgt
    44101 ttaaaattat aaataagtat tttactgaat cggttgttac taagatgagt ttgcagatag
    44161 tgtataatcc aataataact attgttagta taataatagt tttgcttaca atctttatat
    44221 caattctact acctgcaata tcggcttcta atatttctca ccttaatgtg attaaaaata
    44281 ctggagagta taaagtgggc aaaatcaaaa gctcaagact tattaaaatg atttttaaaa
    44341 ctgaaggagt acttgcatat aaaaatatta ggagaaatag aaaaaaattt ataataactc
    44401 ttttttcttt aatggtaagt ataataatat ttatatcttt tagtggcttt acattacttt
    44461 tactaagagg tgaagaaatt agaaattctc aaagaaaata tgatttgtat ttaactgcaa
    44521 agaacacagc taggtctgta gaggatacta taagtgaact agaagatatt gatgggataa
    44581 aaaactttga attggcaaca ggacaatatg tgtctataag agtaagtgaa aataaaataa
    44641 ataaatccaa ggaagattta atcaggaaat attatcaaaa atataagatt ggagatagtt
    44701 atgaatatga tttcagtaat aatgaattaa tttttccagg agattttgca gtaaaaaaca
    44761 aaataaataa cttagtacag ggctctttta ataaagagag agcaattgaa gaaaatggtg
    44821 ttatattagt tagaaaaagt gcatttgaag aaaatgggaa gaaaggtgta gtagaactta
    44881 ccaactataa agtaggagat acagtaaatt gtgaatatct tgatgaaaat gccttaagta
    44941 aaaaagtaaa ggtcaagatt cttgctataa ctgacgaaga acgtctaggt cttggttatc
    45001 aaaatatggg gttacaattt ataacttatg atgaagttgc taaaaattta aatttaaaat
    45061 taagtaaaag ccttatattt attgattcag gaggaagcat taagactaag aaggaaatag
    45121 aagctttggc aaataaaaat aattttaact tttatgatga aagtgcaagc aatgaaagtg
    45181 aaaaacaaga tttaaaggtt attaaaatat ttgtatatgg ttttatagta gtgatatctt
    45241 tagtgagtgt tacaaatatt ctaaatacag taagtacaag tataaatcta agaaaaagag
    45301 aacttgcagt aatacaatct ataggggtta ctcctaaagg atttaggaaa atgatatatt
    45361 tagaaagttt tatatatggt atactgtcat tgttgtttgg aataccaatt agtattggta
    45421 taattctaat tatgaataaa cttatttcag gtgtaattga attttctccg attataccgt
    45481 ggacagccat agttatctgt atagtaagtg tttttataat aacttttata gcagggtata
    45541 taccaatgag taaattaaat aaagaaaata taatagataa tataagaaga gaaagtatat
    45601 agatataaaa ataattctta actatataaa atattattaa aatcatttaa gtggtataaa
    45661 atattattat atcacttaaa tgatttttta tttatataag aaatctaata ataaattact
    45721 agaggtattt aactatttag aaagaattgg tattatataa agtaagagaa tatataataa
    45781 gaaagaatat ttgattttat tggaaaacaa aagaaaaaga aggtgattac tatttctcat
    45841 ataactgcga agaatatgta taaaagttta gaggaaagaa taaacaagtt tccacaaggt
    45901 gctccaccat ctgatacatt atacaaaata ctaaatgtac tatatacaga acaagaagcg
    45961 aagttagttg ctcagcttcc aataaagcct ttcagagtaa aaacagcagc taaaatttgg
    46021 agtgtaagtg aaagtgaagc atatagagta ttggataaac ttgctagtaa ggctctgata
    46081 ctagatatag aagataataa agggaagaaa tatataatgc ctcctccaat ggctggtttt
    46141 tttgagtttg ccatgatgag aacgagacac gatatagacc aaaaactttt ggcagagctt
    46201 tactatcaat atatgaatgt agaagaagat tttatcaaag atttatttta ttcaacagaa
    46261 acgaagcttg gaagagtata cgttcaagag gaagttttaa ctaatgataa tgaggttagt
    46321 attttagatt atgaaagggc tactcatata attgatgaat caactcatat aggtataagt
    46381 atgtgttact gtagacatag aatgcaacat gttggaaaag cttgtgatgc tcctatggat
    46441 atatgtatga cgtttgacaa tgtagcaaat tctttaataa ataataaatt tgcaagacgt
    46501 gtggataaaa tagaatgtaa agaactactt catcaagcat atgaacataa cttagttcaa
    46561 tgtggagaaa atgttaggaa aggggttacg tttatatgta attgctgtgg atgttgctgt
    46621 gaagcaatgg tagcagctaa aagatttgga aatcttcatc cagtacagac aacaagtttt
    46681 ataccaaata taaaccatga aaattgtgta aagtgtggta aatgtataac tgcatgtcca
    46741 atagatgcga taagcaaggt taaggaagat ggtaaagaat atataaaaat tgatgaggat
    46801 agatgtctag gttgtggagt ttgtgttaga aattgccata aaaatagcat tatgttatta
    46861 aagagagatg aaaaaataat aactccagca aattcagtac atagagcagt tcttatggca
    46921 attgaaaaag ggcagttaca aaatttaatt tttgataata atgctctggc tagtcataga
    46981 gctatgggag ctatactgtc agctatatta aagcttgaac cagctaaaaa gattttagcc
    47041 agtaaacaat taaagtcagt atatttagat aaattgttaa gtatgaatga taaatagaaa
    47101 ctaacttaaa aaaggcaaag ttttagactt atctatatct taataaactt atcatttaat
    47161 atttaaattt aatttttaca catataaaac ttataactta aataaaaaaa ggctatattc
    47221 aattaatatt acttgaatac agcctttttt agttatgtta agctatttct tttatttgct
    47281 taaatgagtt ttcatttcca tttccaccaa tttgtgttac agatttaaat ctcatagttt
    47341 taaatagttc tttttgatta taatctagtg agtttcctac tagcattaca ggagatttag
    47401 tttttccagc caaaactcca acagataatc cgtctattaa atcatcttgt ttattcattc
    47461 catttttagc aacatataaa ttgtctatct tagaatcctt gtaaaattct tttattactt
    47521 ttgcattagt ttcatttcta gtgctacctg atattctctg tggattttga agtttacttt
    47581 ctatattttt agaaactgta tattctccac cgattacata agatttatca atctttttgt
    47641 ttttaaacaa gttatttatt tcagttatat tactattttc attagtaagt attataggca
    47701 tatcattttg agctgataca gctccaatgc ttactgcatc ggctaatcct ttttcgccat
    47761 taactactga aatctttgat atattgctaa tattgttcaa ttctttagca atgttcatag
    47821 aagtttgata tctatcactt cctgaaattc tagatacaga gatattgttt gattttatag
    47881 tatctaaaga tttttcattt atagaagctt ctccaccaac tacatataca tttttaactt
    47941 ttaatctctt taattcagcc aaagtttttt catttatttg agagcttcca gtcaataata
    48001 taggagcgtt tttcttgtat gcaaatggtg ttgcagccaa agcatctgca attgctgaat
    48061 cgtttattaa aactgcattt tcagcatttt cccaacctgc ttgacttatc ttaatagaag
    48121 tttcatatct atcagttcct gtaagtgcac ttgcatttac ttcatcttga tatgtaacct
    48181 caaatctagc atttggactt cctaagaaaa caattacgcc tccttttggc aatctaactc
    48241 tattgttctt tgtaactaaa gaatagttta caggaacacc tttatcatca taaacagcaa
    48301 aagctgaatt ttgaggaaga ttaacttcta tttttttatt tgcgatatcg tcaccaatct
    48361 tataccattt tgcataacca ttggattcta gttcacaagt aaaagatttt tctgctggca
    48421 aattagttat agaatcttca ctaacatacg ttagtgttcc aaaagataaa tactctgtac
    48481 cattttcttt atgaagttta atgtcggata aatcacgacc tattacacca ggtatttcta
    48541 taaaagcatt tgagttattc tcatccatta ttttagtgtt aactatataa cctggagttt
    48601 catcagaaag actaaaactt gcttttacag agccaaacat atagctttga gaagtatatt
    48661 tttcatccac tagatagtaa cctttgccac ttcttttttt ccacacttct ttgacactat
    48721 ttgatatatt gttgtcatct actttttgag ctacatagta taaacttgct gtctgaccta
    48781 atccagggac atcatcataa gagctcatat ttaaatatgt gatattattt ttttctttta
    48841 caaattttaa acaactatta cctttttcag atacaaatct atcttgacct atgtatacgt
    48901 atttatcttc aggtccattt tctatataag gagaagatac tgttaaagta ccattatcat
    48961 taacatctac ttttatcata tttgaagaag cataaagacc actattttct tttaaagagc
    49021 taggcatttt aacttgttgt ggcttactaa atgttttatc aggttttatt tctttaattt
    49081 tacctttttc ttttaatgca gatagtaaga tttcttgtcc aattatttca ttaagttgac
    49141 tacttccacc agaagaaaga actgcaactg ccatattttc atctggaaga actataagat
    49201 tactgtggaa taataaagaa tctccacctt ttgtaagagc ttttaaatta tactgattaa
    49261 atggataagt gttgacacaa tcccacccta aaccatatcc aagtatagag tcttctcctt
    49321 caggccatag accatttaaa tattctttgt tttccatagc ttttactgaa gcaggagaaa
    49381 gaataccatt tgaatttttc ataaatgttt gtgcaaatgt acatagattt tctgcacttg
    49441 aatataaacc acctgcacct atagcattaa ggttatcttg tggaactgca tcttcccaat
    49501 aaggtacata tgcttttgct agttttgaac tatcaaaact attttctgga gtttttgtat
    49561 tttgtaagtt aagtggatta tttatgtatt tatcaagaaa attagtaaat gacataccag
    49621 atactctttc tactaaaatt tcagctaaag taaatccatc attacaataa acagaaaaag
    49681 ctcctggttt agcttttaat ctttgttttt gtaattcttt taagaaatta tcatgaccat
    49741 aagaatcatt gtctgctaat aaaatagtgt ttttaaaact acttcccatc aaccctgatg
    49801 agtgatttaa taacattctt ggagtgattt ccttgtatct atcatcagcc attttaaatt
    49861 caggtatata ctttacaaca ggagtatcta aatttaattt tccatcatca accaacttca
    49921 taacagctgt tgtagtaaac attttactga tagatgcgat actatacata ttatccttat
    49981 ttaagttttt gttatcctgt ttgctgtaaa ctccaccatt accagaaatc tcaattttgc
    50041 cattatcaat caaagcatat tgagcactta cagcaccata ctttgatagc aaagtttgga
    50101 ttttctcttt agcaagagct ttagtttttg tgtaagattc cgcgttttta ctataatcat
    50161 gtaattctat atcagaactt tgatattttt cggaattatc ttttgtatta tcagcaaaag
    50221 taatatttgg agcagcagta aataacatac tcaatactaa tgtactagat aataatttag
    50281 agagcctttg cttaaacata ttacattacc tctttccctt tttaataatt tataattagc
    50341 ataacattct taattatttt aatcaacaga ttattatcaa taaaaaaagc ttttataagt
    50401 atagcttttt atataacaga gaaaaatata aaaataatat ttgttgttgt ataaagaata
    50461 aagggtatag gtaatatata aataaaatgg aggaatttta gacatatgta taagaatata
    50521 aataagaagc aacttaaaga aatgatgaaa aatgaaaagg atgttttatt attagatgta
    50581 agaactaaag atgaatttaa ggaatataaa atagaaaatg ctataaatat atctttacaa
    50641 gagttaatca ataatataga tgaaatttac gattataaag ataagaaagt agtagtttat
    50701 tgcagaagtg gtcatagaag tgttactgca tgtaatttgt tagctgaaga aggttttgag
    50761 catttatata att
//
LOCUS       DAEMGG010000013        50752 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEMGG010000013 DAEMGG010000000
VERSION     DAEMGG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.013"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(169..2139)
                     /gene="ftsH"
                     /locus_tag="KPK24_000781"
     CDS             complement(169..2139)
                     /gene="ftsH"
                     /locus_tag="KPK24_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG2298488.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2173..3561)
                     /gene="tilS"
                     /locus_tag="KPK24_000782"
     CDS             complement(2173..3561)
                     /gene="tilS"
                     /locus_tag="KPK24_000782"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG2298489.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3744..4172)
                     /locus_tag="KPK24_000783"
     CDS             complement(3744..4172)
                     /locus_tag="KPK24_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG2298490.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4189..4995)
                     /locus_tag="KPK24_000784"
     CDS             complement(4189..4995)
                     /locus_tag="KPK24_000784"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG2298491.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5197..5682)
                     /locus_tag="KPK24_000785"
     CDS             complement(5197..5682)
                     /locus_tag="KPK24_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG2298492.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5733..6392)
                     /gene="spoIIR"
                     /locus_tag="KPK24_000786"
     CDS             complement(5733..6392)
                     /gene="spoIIR"
                     /locus_tag="KPK24_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG2298493.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6537..7217)
                     /locus_tag="KPK24_000787"
     CDS             complement(6537..7217)
                     /locus_tag="KPK24_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2298494.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7201..8091)
                     /locus_tag="KPK24_000788"
     CDS             complement(7201..8091)
                     /locus_tag="KPK24_000788"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG2298495.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8209..9759)
                     /locus_tag="KPK24_000789"
     CDS             complement(8209..9759)
                     /locus_tag="KPK24_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG2298496.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9853..10119)
                     /locus_tag="KPK24_000790"
     CDS             complement(9853..10119)
                     /locus_tag="KPK24_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG2298497.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10267..11127)
                     /gene="yabG"
                     /locus_tag="KPK24_000791"
     CDS             complement(10267..11127)
                     /gene="yabG"
                     /locus_tag="KPK24_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG2298498.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11211..12149)
                     /locus_tag="KPK24_000792"
     CDS             complement(11211..12149)
                     /locus_tag="KPK24_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG2298499.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12259..12420)
                     /locus_tag="KPK24_000793"
     CDS             complement(12259..12420)
                     /locus_tag="KPK24_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298500.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12430..12927)
                     /locus_tag="KPK24_000794"
     CDS             complement(12430..12927)
                     /locus_tag="KPK24_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG2298501.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(13042..13752)
                     /locus_tag="KPK24_000795"
     CDS             complement(13042..13752)
                     /locus_tag="KPK24_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG2298502.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13928..14458)
                     /locus_tag="KPK24_000796"
     CDS             complement(13928..14458)
                     /locus_tag="KPK24_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298503.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14475..14705)
                     /locus_tag="KPK24_000797"
     CDS             complement(14475..14705)
                     /locus_tag="KPK24_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298504.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14720..16114)
                     /locus_tag="KPK24_000798"
     CDS             complement(14720..16114)
                     /locus_tag="KPK24_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG2298505.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAMPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16240..17493)
                     /locus_tag="KPK24_000799"
     CDS             complement(16240..17493)
                     /locus_tag="KPK24_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2298506.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17544..18698)
                     /locus_tag="KPK24_000800"
     CDS             complement(17544..18698)
                     /locus_tag="KPK24_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2298507.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(19258..19827)
                     /locus_tag="KPK24_000801"
     CDS             complement(19258..19827)
                     /locus_tag="KPK24_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2298508.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            20030..20257
                     /locus_tag="KPK24_000802"
     CDS             20030..20257
                     /locus_tag="KPK24_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2298509.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20476..21219)
                     /locus_tag="KPK24_000803"
     CDS             complement(20476..21219)
                     /locus_tag="KPK24_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG2298510.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNATSQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21387..22259
                     /locus_tag="KPK24_000804"
     CDS             21387..22259
                     /locus_tag="KPK24_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2298511.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22259..22924
                     /locus_tag="KPK24_000805"
     CDS             22259..22924
                     /locus_tag="KPK24_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG2298512.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22945..23802
                     /locus_tag="KPK24_000806"
     CDS             22945..23802
                     /locus_tag="KPK24_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2298513.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23958..26504)
                     /locus_tag="KPK24_000807"
     CDS             complement(23958..26504)
                     /locus_tag="KPK24_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2298514.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26504..27190)
                     /locus_tag="KPK24_000808"
     CDS             complement(26504..27190)
                     /locus_tag="KPK24_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298515.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27273..28262)
                     /locus_tag="KPK24_000809"
     CDS             complement(27273..28262)
                     /locus_tag="KPK24_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2298516.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28249..28935)
                     /locus_tag="KPK24_000810"
     CDS             complement(28249..28935)
                     /locus_tag="KPK24_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2298517.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(29435..32641)
                     /gene="carB"
                     /locus_tag="KPK24_000811"
     CDS             complement(29435..32641)
                     /gene="carB"
                     /locus_tag="KPK24_000811"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG2298518.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(32834..33883)
                     /gene="carA"
                     /locus_tag="KPK24_000812"
     CDS             complement(32834..33883)
                     /gene="carA"
                     /locus_tag="KPK24_000812"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG2298519.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(34140..37346)
                     /gene="carB"
                     /locus_tag="KPK24_000813"
     CDS             complement(34140..37346)
                     /gene="carB"
                     /locus_tag="KPK24_000813"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG2298520.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(37399..38442)
                     /gene="carA"
                     /locus_tag="KPK24_000814"
     CDS             complement(37399..38442)
                     /gene="carA"
                     /locus_tag="KPK24_000814"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG2298521.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(38468..39184)
                     /gene="pyrF"
                     /locus_tag="KPK24_000815"
     CDS             complement(38468..39184)
                     /gene="pyrF"
                     /locus_tag="KPK24_000815"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG2298522.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(39560..40195)
                     /locus_tag="KPK24_000816"
     CDS             complement(39560..40195)
                     /locus_tag="KPK24_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG2298523.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(40219..41133)
                     /locus_tag="KPK24_000817"
     CDS             complement(40219..41133)
                     /locus_tag="KPK24_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298524.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            41734..42963
                     /locus_tag="KPK24_000818"
     CDS             41734..42963
                     /locus_tag="KPK24_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG2298525.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWNIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(43168..45549)
                     /locus_tag="KPK24_000819"
     CDS             complement(43168..45549)
                     /locus_tag="KPK24_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG2298526.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(45521..46246)
                     /locus_tag="KPK24_000820"
     CDS             complement(45521..46246)
                     /locus_tag="KPK24_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG2298527.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKYVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(46236..46691)
                     /locus_tag="KPK24_000821"
     CDS             complement(46236..46691)
                     /locus_tag="KPK24_000821"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG2298528.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(46973..48154)
                     /locus_tag="KPK24_000822"
     CDS             complement(46973..48154)
                     /locus_tag="KPK24_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2298529.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(48144..48836)
                     /locus_tag="KPK24_000823"
     CDS             complement(48144..48836)
                     /locus_tag="KPK24_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2298530.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(48970..49686)
                     /locus_tag="KPK24_000824"
     CDS             complement(48970..49686)
                     /locus_tag="KPK24_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2298531.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            49811..50545
                     /locus_tag="KPK24_000825"
     CDS             49811..50545
                     /locus_tag="KPK24_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG2298532.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
BASE COUNT        16025 a         9271 c         5687 g        19769 t
ORIGIN      
        1 tataatttta cattttattt aaataatttt aaaataataa aaaaactgaa agtatcaatt
       61 aaattaatac atgcaaatat aatttgctta ttaatttaat attatacttt cagttttagt
      121 atttaatcca catcagataa taatcttaaa atgaccaagg tcttaaaact atttatcttt
      181 atcagattta tcttctaaat ttttatttat atcaataaca ttgtttcttt cttcttcatc
      241 tttttctaat tttatagtta attgttcttc aacttcttta gaagaatttt cctctgtaac
      301 tgcattttca atttcattca gaggtaactc tttattaaat gcttttacaa attgttctgc
      361 atctaaagtt tcatatataa gtaaagcttg tgctacatac tctaatctat ccatattttc
      421 ttgtaatata cttcttgtct tatcatatgc accatcaact atacgctttg tttcttggtc
      481 tatctcaaaa gcaacttctt cagaataatt tctcttactt gaaaaactat tacctaggaa
      541 tacttcatca tcactgtcaa atgacattgg accaagttta gaactcattc catactttgt
      601 taccatactt cttgcagtag ctgtaactct ttctaagtca tttgaagcac ctgtagatac
      661 atccttaagt actagctctt ctgctactct accaccaagt aaaactacaa tattttcttt
      721 catttcattt tttgtagcat agaacttatc ttcaacagga agttgcattg taaaccctcc
      781 agctcttcct cttggaacta ttgtaacttg atgaacagga cttacttctt caagtacatg
      841 ggcacaaact gcatgccctc cttcatggta tgctgtaagt cttctttctt tctcacttat
      901 tactttcgat tttttagcca cacctgctat aacctttgtt atagcttctt ctatagtttc
      961 catcttaatt ttcttttctc tttttctagc tgttaatatt gcagcctcat tcattaagtt
     1021 ctctatatca gcaggagtaa atcctggtgt tcttctagcc aagacatcca tttttacatc
     1081 atcacttaat ggtttatttc ttgagtgaac tttaaatata gcctctctac cttttacatc
     1141 tggtgtacca acaacaactt gtctgtcaaa tcttcctggt ctaagtaaag ctgggtcaag
     1201 tatatctggt ctatttgtag ctgccatgat tattatacct tgatttaccc caaatccatc
     1261 catttcaact agaagttgat taagagtttg ctctctttca tcatgacctc caccaagacc
     1321 tgcacctctt tttctaccaa ctgcatcaat ttcatctatg aatatgatag ctggagcact
     1381 tttcttagcc tgttcaaata aatctcttac tcttgaagca ccaaccccaa caaacatctc
     1441 aacgaaatca gaaccactta tactgaaaaa gggaactcct gcttctcctg caactgctct
     1501 tgatagatat gttttaccag ttccaggagg tcctaccata agcattcctt ttggtattct
     1561 tgcacccaat tcaatatatt tcttaggatt ctttaagaaa tctactacct cctgcaaatc
     1621 ttctttttcc tcatctagtc cagctacatc tttaaatgta actctagttt tttcatcatc
     1681 tttatgaacc ttagcttttg atttgccaaa actcataacc tttccgcctc caccttgaga
     1741 ctgattcata aacacgaacc aaataatcac catgaagaat attaacagta atgtaggcaa
     1801 catttcgaca aaccaaggtg tagatggctt agcttcacca ccaaaagtta gtttcccagc
     1861 cttagcttgg tctaacacct catctgcaag tttatttccc atcacttctg ttggaacata
     1921 tgacttaaac tttgtatttg tatcttttat agtaccttct acagaagttt gatttacaaa
     1981 gtataatctt gatatattct catctgttaa ttctctatat acttcagaga attttaaatc
     2041 ttttattttt tctgtaggct tacctgagaa ttgaactata cctactataa ttataaaaac
     2101 aagtagataa aaacctgccc cttttagcaa cttattcaat atagaagccc tcctttcata
     2161 cattgctttg ccctataatt tgttaaaatt aatttttaat acttcttttg tgttttcatc
     2221 aattttataa ttttcactga ttttataatc tccaacacat attattcctt cactatctgt
     2281 gattatagga attttacatc tttcttctct tgggattttt aaatctatga ataaatcttt
     2341 cactttttta cttcccatag caagctttat cttatcacct tgccttctgc ttcttattac
     2401 tataccccct tttaccttat taaaatcaaa ccctttagat gaattgtcta atttcatact
     2461 cttataccta tctatgctca ttacttgggt ctcaataatt tgttttaatt cttttatttt
     2521 aataaaccca ttactaggta cattgtaata aaattcaatt tcctcattaa caatttcctc
     2581 attcgttaat attatactgt ctttttttct ataaacaaat atatttcttg gaagagttaa
     2641 cattttattt acttttgaat tatcttctaa agacattata tcttctatat gtctttggtc
     2701 aacaaaatta gtatctccta gtatatgttt tatggaattt ctaagaactc taactttgat
     2761 agcatcatgt aagcaaacaa aatcgtctaa atttatctct acaaagcctt tttcttttat
     2821 atttgaaact tctctaaatt tagcttctgc ctctttctca atataatcgt tatcactttt
     2881 taaactattg ctcatcctca ctatagattc tattacattt gaattaaaat tatccttcat
     2941 atatggtaaa aggtctaacc ttatcttatt tctagtgtat atattttcta aatttgtttt
     3001 atctatcctc ggatttaaat tataagcttc acaatactcc tctatctcat ttctttccac
     3061 gtctaatatg ggtctaatta tgcaattatc tctaatataa tcaattccct ttaagccttt
     3121 taaacctgtt cctctcatga tacgcatcat aaccgtttcg gcttggtcat tgagattatg
     3181 acctattgct attttattgg ctttaatctt atccttaatt tcataaaaca tttcatatct
     3241 tagcttcctt gctccttctt ctaatgataa tccctcattt tcacaatatt ttggtacatt
     3301 aatagacttt acaaagaata taattcccat atcctcacaa agtttagata catataatgc
     3361 atctttttga gcttctatcc ctcttatttg atggtttaaa tgtgctgcat atatttctat
     3421 attaaaatct ttttttaatc tatttagtac atgcaataaa caaactgaat caggtcctcc
     3481 agaaagacct aaaactattt tatcgccttt ttgtattaaa ttatgtttat ttatagtact
     3541 tagtacctta tcaaatatca tatttatccc tcttacactt agttttattc acttttaagt
     3601 atactttcta taaaaaaacg ctaaaccctt gtatcaattt taagaaatta aagtttaata
     3661 taaatataat aataccaaat aaattaaata aaaggtagtt ttttcactac ctttataaaa
     3721 tttctggaaa atagccatag ttattattta gtacctatgt atatatctat tttattccta
     3781 cctaaaaata aatcttgtac ctttatatta tagtctattt tcaattttat gtgttcccct
     3841 tcttgtatat ttatatctaa ttctcttgta tcaatatcaa ttaataaagt accttgtggg
     3901 gtcacatact ttgtagtact actttctcca catttaaaca gcattgttga gttaatagca
     3961 ccaaactttt tgatactaac ttgattttta agtatcttta tagtattagt tatttctata
     4021 tcttcttctt tttccttgta aataacatat attccatcat ttttataaaa aattttacca
     4081 aaggcagtca tctctatggt gtccatattt cctttttcat catattgtct agtatttata
     4141 cttattttta cactcaacga tttttcctcc atagattctt catataaact agtatttttg
     4201 tatttctact ttttgaatct tatcaatttt tttctttaag aactctcttg ctatatctga
     4261 aaatttttca ggtatatctg atacataata ttgataagtt ccatgaattt ctgtattgtt
     4321 tataatattt tgcacctcta aaatcttttt taaatccttt gcagtctctt tagctggatt
     4381 aacaagtttt atatgctctc caacttcttc acctattgtt ctctttaaaa taggataatg
     4441 agtacatcct aacactaaag aatctatatt tttttctttc aattcttgta aatatatctt
     4501 tgctgtcagt ttagccactt ctgtatttgc ccatccttct tcaacaatag gcacaaataa
     4561 aggacatgct ttattaacaa tttctatact ctcatctatt ttagatattt ccaagttgta
     4621 tgcttttgaa cttatagttc cttcagttcc tattattcct acaattttgt tttttgtaga
     4681 acttacagct gttctagctc ctgcttctat aactccaatt atcggtatat catatttttc
     4741 ttgagcttct tttaagcttc ttgcagtagc agtgttacat gctataacta tagcttttac
     4801 accttttgtt tttaaaaagt ttatagcttg aaaagtatat ttaattatag tttctttgga
     4861 tcttgaacca tatggtattc tggcagtatc tccaaagtat attatatcct catttggtaa
     4921 tatcttcatt atctctttta gaactgttaa tccacctaat cccgaatcaa aaactcctat
     4981 tggtttattg ttcatcttta tacaacacct actttacatt tttgtgttta acaacaactt
     5041 atttatcgct aaaatttata tttatattca taattaatat attattacct aaatgattat
     5101 tatagtttat tttacacaaa aaataaagga gctatcttaa attagaaaat aatttctaca
     5161 aaaagacaac tcctttcact acttaaaaat ttaaatttat tttgcaaaca catgatttcc
     5221 tattctcata taaggattca atctatttat ccatttgcaa gtggctattt ctggattcca
     5281 aaaatatact gctttattag ttgggtcagt tccataaaga gcttcctgag ctgctttacg
     5341 tgctgagtct gttggtgcca tctttattga accatcaagc acaactgaga aagcattttt
     5401 ttgatatatg acttcttcta ttgtgtctgg aaatctaggg tccaatactc tatttattac
     5461 tacagctgct acagcaacct gtccctcata gctttctcct ctagcttctc ctgcaaccaa
     5521 cttagataat aacatcaagt cattatcaga taaatttatt acttctttct ttgctatatt
     5581 tttttcttgt ttgtttactt tcttaatatt ttctgttggt tcatatgcaa atacctcttt
     5641 aataggcata aatataaaag tcaaagctaa tagtgtagcc atcagtttac tccacctgtc
     5701 catgattaaa accactcctt acattcttat ttttaaaatt ttttctctaa atccttgact
     5761 acttcaagta ctttaaattt aatttttact ctacttgttt tttttggtgt atcttgtttt
     5821 attaacttat attctttatc agttaaaact tccttcaact tatcatctgt agatttatct
     5881 atcactccat tagattcatc aacaaaacat agtggaggga acataacaca ccaccagttt
     5941 tttccttcac ctttcccaat gataatcttt aatgctttat attcaccagc tggaagaact
     6001 atattagaat attgtttagt agggaaattg ctatattgta tacctacctt tacactgtaa
     6061 ttatatccat ttttaagaat aatattttta cttatttctt ccaaactact atattccttt
     6121 tttataatag cttcactttc ttttatactt tttgaatttt gaagctttgg ctgtaaatat
     6181 tttattactt catctctaac ttttagttta agttcttggt cttcatctgt attgctgttt
     6241 gctataacat gaaatcttat taatttatct ttataatctt ttgatatatt gtctactttt
     6301 tttacctcac catttattac tattgtcatt attgatatga ctgatataag acttaatatt
     6361 aatagtccca atctaatctt aatttttctc attgaaaatt ccccctcaag ataaatttat
     6421 atcatcaatt atgtccaatt aaatttaatt tattcataat attttttaat actaataaaa
     6481 aaagctatct taaaatgatt acaatcattt taaaatagct ttttaaacct tatattttat
     6541 aagtctttac taatcatagc cttatctatc ataaactgct ctgctttttc tatatgctct
     6601 gtagcatact tcttggatag ctcactatca cctttaatta tagcatcaag taaatattta
     6661 tgctcttcaa caatattatg tgatgtatca taagcagaca tataggtaac tctaaatcta
     6721 tatacttgtt cccacaatga gtttattaat tggattaatt ttttattatt agttgcttta
     6781 aatatacatt catgaaattc tacatcaagt tttaataacg catctacatc ggattccaaa
     6841 gtgctttttt taaattcttc tgatatatct ttaagctttt taatatcttc atcactaatt
     6901 ctttcagcgg caagatatgc tgctaagcct tctaggcttg ctcttacttc taatacatcc
     6961 ataatatcct ttaatgacat atttgccaca tacgcacctt ttctaggcaa cataacgact
     7021 aaaccttcta attcaagctt tcttattgcc tctctcactg gagtcctact tactcctaat
     7081 tgttctgcta gttgcacttc cattagcctt tgaccaggct ttaattttcc ttctaagatt
     7141 gcttctctta aattctcaaa gactacatct cttaatggct tgtaattatc caagttcagt
     7201 ttagttaaat tctccacaaa tttcaacccc tttttgactg ctattaacaa catacacctg
     7261 tttatatttt tttaataatt ctttttttcc aattagagca tcttctttat ttttaaatag
     7321 tccaaaaact gtaggaccac ttccactcat catagaacct aaagcattat ttttcatcat
     7381 tacttgtttt atatcactta tttctttgtg tttacctata gtaacatttt caagaatatt
     7441 taccatgctt tctgatacag cttttatatc ttcagatttt aaacactcta tcaaatattt
     7501 attatttggt ctttttttaa tattattcaa atctaatcct tgataaactt ctttagtaga
     7561 aacgaataag tctggtttgc atatcaatat atttgtatca catggtaaac ctttgatgtt
     7621 tgttagcttt tctccaatac cttgagctaa ggcaggtctt cctgatatac aaaaaggaac
     7681 atctgcgccc aaattaagtc ctatttcttt taattcatct tcagataatc taagttccca
     7741 taggtgattt aaacctacta atactgcggc tgcattagaa cttcctccag ccatccctgc
     7801 tgctacagga atattttttt ctatgaatat ttctacacct ttttttatat aaaatttatt
     7861 tttaagtatt ttggcagctt tataaacaat attatcttca tctaaaggaa tatctaaact
     7921 agtgcttttt acctttattt catcttcgtc taattctttt atctttacta tatcgtataa
     7981 atctatcgtt tgcattatca tttcaacaaa atgataaccg tcttgccttt ttcccaagac
     8041 atcaatggat aaatttattt ttgctctact ctttagtctt ataaaatcca ttctatcctc
     8101 cttgtttaaa gaataatgca tcactaacaa taatattata ctacaaaaaa taaaataata
     8161 gggtattata ataccctatt atacttaatt actttaaatt ttttaatatt aatctactaa
     8221 tacgactttt ttttctaaaa taagacactt accaggctta attggttcat caagttttat
     8281 atcattaagt tctgcaattt cattttctgt tgtattgtat ttttttgcta tattccagaa
     8341 agtatctcct tctttgcata tatatacgat tatgctagga gctttagata aatcatatac
     8401 accttggtct tctcccttga taataaaatt ttctgctttc ttatctaatg cttcagtaaa
     8461 tctcttaatt ttgataatca agtctatctg gtccctattc aaatcaactt cgactttatc
     8521 aatacatgct gtattaaaca cagatgctgt atcagttaga ttatccattt ctatatcatg
     8581 ttcaaatgga atttcctcac taattttata aaccaatttt aaaccttcaa ctgggacaaa
     8641 taaaatttca acttttatga ttccttgtat aatgctttta tttccttcaa tataactatt
     8701 ttctatagac atggttggac aaacgcttac aatatctttt atttgtatat catcattatc
     8761 atttcttatg ccttctctaa ccataaatgt ttcttcacta cgtcttaaag tcttatttaa
     8821 ttgaataggt ttgtggtcaa attttattat cttttgaggc gaatatgcat cttgtaatac
     8881 ttctcttgta acttcatctg taacttttac cttacaacaa gccatacaat ctatttctaa
     8941 aagacctgtg ttgctttcac tattttgctt gaaaatatga ttaaaatctg acatagacaa
     9001 caatacttct tcagtcatac catcactaac tccagggact tctacaaatt gagtaaactc
     9061 aatacctact ctatccaact ctacaagttc accctcatat gtacaagcta acggatttat
     9121 ttctaatact ccaccaataa taactttatt gtcagtcact ctactttctt ttactttaac
     9181 acatggattt aaacttatta tagattgaat ttcctctgtg tttattgtta tagtatctct
     9241 tatagaactt tctgcttttt ctattccaac tatatcttga aaacaaattt cctttctgtg
     9301 cttttgtatg ccttccactt gcgccacatc ttttacaatg tctaaccttt gcttttcaaa
     9361 caaacttccc cttatattca taagagctcc tactttaatc tttctctcat tcattatagt
     9421 acaatccata tgctctactt ctgaaaataa catgtattcc atatcttgaa caacattgtc
     9481 tttttctatt acttcgttta tatctacttt tccgtctaca ttagaaatag tatttttatc
     9541 atctgcaata tagattacat tataattaaa actacctctg caaagtatct tcccatctgc
     9601 catttcaatt tttttaagtg atatgtatcc ttctgttttt acaatttcat atacatctaa
     9661 ttttttgtct ggtacaactg cctccgcttc aatgaatgtt tgaaattttc caaagtctat
     9721 tctattgtca actttaatta catctttaat taattccata gtctacctcc ttaagtatta
     9781 taaaaatatc acactatatt tatatttaag gagcaaaaaa aaaattacac ctatttttaa
     9841 ataggtataa ttttaactta tttgtaattt atcttgatct ctaaaaactt gcaactttac
     9901 attagatgtt aataaatctg agtaactata agatactcta ggatatccat tactttcatc
     9961 atctaacttt ataacaaaca cgcttggata aactttttct agtataccct cttttgtaat
    10021 aatctgcttt cttcctttat tagctttcaa caatattttc tttcctatat gtctctctaa
    10081 gcttactctt atcttatcta gagtttgaac agtagccaca atatcacctt ctttaacgtt
    10141 tatatgttta cataatatca catataaaca tttttgtcaa atgtttaaac taattatttt
    10201 atatattttt tgcaaaaatg tcaatactat ttttgaagat atacagtata attatgttaa
    10261 tttttgctaa tgtaatattg tttttggcat tgcccatcta aattttcctc tggtttcaga
    10321 accaccaatt cccttaacac ctgtaatagt ctgctctata acctcatcaa taggaactac
    10381 tacatcaatt cttgaacaag caattttctc cactataaaa acagggtcta atgcatgtat
    10441 cattatcctt ccaggcgaac ttgcatagtt tgctcctgca cttattattt tttcataatt
    10501 tgattggcat gctcctgcga atatgacaag attatccaaa tttggttgcc atttacgtgc
    10561 ttcttttact gttttaacaa aatttgaaga gtttctatag ttactcatat cttctatatc
    10621 tcctcttttt attgtcattg catcatgacc tgttattact agtatatccg gattatattt
    10681 ttctagtagt gctcttactt ctttatactg attttgctct gaaacagcta cacctactgc
    10741 aggtattcca agttgagcat atacatccaa gcaaattttt aaatattctt tatctccatc
    10801 tatctgtagt acttttccag gcattccata tgtgttagga tttaattgaa gttttggaac
    10861 tgctctagtc attctttttt gtctttcttt agcttttctt actgacttat ataataaatt
    10921 ttctacattt ttatctatta atatatcttt tatatcaggt gcttttacta attctaggtc
    10981 atcaatataa gcatctgcta ttactctaaa tgcaattccc tttagtatag ctattttctc
    11041 attattttca tctacaccaa aagagactat tttaaatact atatctttgt tatgtgactt
    11101 tctagctaca atgtctccta ccttcatagt taaacctcca ataaattttt atcttagttt
    11161 atactatgtt cgatataaaa ctttgtttcc taatgtcaaa aaaacatttc ttataatact
    11221 tcttttatca ctcttaaagc atttttatag tatatctttt ctatatcatc ttcactaaac
    11281 ccttcttttt ttagaggttc atacagttta cccatctgag atatatcttc aatctcaact
    11341 tttgaatcta ttccatcaaa atctgaccct agagatacaa catctattcc tccaacattt
    11401 acaatatgtt ttatgtgtct aaccatatct tcaagtttac tttcactttt atcacttaaa
    11461 aataagtgga agaagtttat acctgtaact ccacctttat ttgctaatac tttaatcata
    11521 tcatcagtta aatttctaga atgattcatc attgctcttg aattagaatg tgtagcaatt
    11581 attggtttag aagaaatctt tgcaatttca taaaatccac catctgaaat atgagaaaca
    11641 tcaaccaaca ttcccaattc attcatctta tgtaccactt ctcttccaaa atcagtaagc
    11701 cctttttctc tatattctac tttattatga ggaaagctca attcattcac atgattccaa
    11761 cttattgtca tcattctaac ccctaagtcg taaaatttct ttaaattttc taatttacct
    11821 tcaagtactg caccttcctc aatagataaa agagctgtaa gtttaccttc tgattggttt
    11881 ttcataattt cttcatagtt agtagctaaa gctatttcat cactattttt tttcatttcc
    11941 tcatgaaatt tatttgccat actcatgcaa taatcaaaag gatgttttac ttcttcagta
    12001 tcaacaaaaa gagcaaatgt ttgagctaat gaatccccct ttttcaatct gtctatatca
    12061 acagaatatt tattagactt aagttcactt gtttctacat tttccattaa ttttgctata
    12121 gtatcacaat gtaaatcaat aaatttcata tttatacccc ctgtatattt tatatttata
    12181 tctaaaaata gaaatagttg tccttataga caactattga caactatttt ttaatattct
    12241 ttttaatatt acttaatctt attcttcttt taagttaggt attacttttt taacttcttc
    12301 aatttgatgc catgttttat ctatattatt ttcactaata tattttttta aatcagttaa
    12361 agatgtaaaa tctttaactg ataaattgtc atcatcatct gcaaatctta aaataaacat
    12421 gagactccct tactttccac aacatttctt atattttttt ccgcttccac atgggcatga
    12481 atcatttctt cctatttttg cttctttaac tattgtttta gacttcttat aagcctttat
    12541 tatttctttt ctttggtctg ctggtagtat atcttcccaa ccttgtaaat tatatagcca
    12601 atgagcctca acagttacca tattgttata taatttttcc caatctattt ttatagaaac
    12661 ttctttatca gcttctactt cttctaaaac tatatcttct tttaagcttt cacttattcc
    12721 atctatgaat cccatgaaaa attcattagt agttccaaat ttttcagcca attcacttac
    12781 tttaccttca acaacatcca ttttattatt taatatttca ttgtatattc cagcttcaac
    12841 tttcaaatat tcttcccaga attttacttc tgcttcttga ctttcatgat tatcacttaa
    12901 atttctccat tcagtatata aactcatact ttttcctcct aagtatacct atatttttat
    12961 agtttttata gtataaaatc ttttagttag attggaagtt tcttcttttt gattatacca
    13021 tattctattt aaagttaact cttatattat atcataatta aacaattctt ttaatacttt
    13081 tatagcatac tcttggttta taaaataagg gcttgtcgca attgttatta ctttggcttt
    13141 tttaattaag ttttttatct tgtaaaattt gagttcaaat ggtatatagt ccatatcact
    13201 tgaaaatata tccaaatcta tgtcaagaac aaactctgat tctacttcta aatcaaatcc
    13261 atatagacta tctattatta ttaattctga aaatatgtta tattttaaag caggttttat
    13321 aaaacttccc acatttaaaa cttcatttgt atatctaaat acatctttta gattatttac
    13381 atctacatca tagttctcag gttctctagt atccttatgt tggtctacat gcactaactt
    13441 gcatcctttt gtaaactcac cctttaacaa agactttgcc caaaaataaa atgcatggtt
    13501 atgattgtca aacacatata tctttttatt tcccaggaca tattcgacca tattctccaa
    13561 tccttttgct tttatttcct tattttcatc tacttcatta aatactacct cattgcctaa
    13621 ccttacatca tttaatgtac catttataag ttttggaacg tatatgctct tattttctct
    13681 tttatcatat gaaaatacat tatttcctac tggtttttct atataaaatc cactatattc
    13741 ataattttcc ataaaacttt atttcctcca ttgttatata aatatataag ttataatttt
    13801 aaaaattaaa atgcacttat tgtaaatcat atcatctatt atagaaaaca tcttatataa
    13861 ctaaatataa tatcttctct caatatgtat tatacaataa gtgtaaaata tatttatata
    13921 aaatatacta cattcctata acaaaaccat cagcttcaat tggaagataa ctaactcttc
    13981 tattcaactc cacctttcca aaaaatacta cccaatatgg tctaaaaata ctttcaattt
    14041 taactatttc aatctctggg attcctccca taaacctatg agttaatctc atagccattt
    14101 tatttgcctt tgcttttact cttcgttcgt ccttatcact atactgcaca atattacaat
    14161 ccacattttt tacaacttca tatgtaggaa aatcttgtac tatactacca gaacctgtag
    14221 aaccattcac taaaattttt aattcattta tatcattaga atatctccta ttcttgaaaa
    14281 tggatttcgt tttgtgaatt ttaagtgtga taattttaaa ctctatataa tgaagtctaa
    14341 cttcatgagc atatttgttc tttaatatta gcttactaaa taaagaattt tttccacttt
    14401 tagctatctc tatagcttct tcttttgtaa ttcttatagg taacgctctt atatccataa
    14461 aatcattctc tttcttattt ttttcttgtc atttttaaaa ctctatatat ataatcaaaa
    14521 aatattacaa ttacaactga cataactcca tatggaatta tttttccaac tataatatca
    14581 tttctagtca atataccata aaatataaat cctgttatta aaaagtacaa taatactaga
    14641 aaaccgtcta ttccaagcca ttttatttct aaatcttttt ttaccttaga cactaattta
    14701 gacatagcag tttctccttt tatagattat tttgtgtatt taaattttca cctattctat
    14761 taccaatcat ccatgcaata aatgaaaaag gcatagctat cacaagaggc tctaactcaa
    14821 agctctgaac tggaaatatt ttaaaataac caaaagtcac tccaccaaca atcattccta
    14881 aaataccacc catagatgtt cttcttccat ttccaagaag cccacctact ataggtacaa
    14941 atgccgaacc catccaaata cttcctacaa atatacatgc atcatataca ttttcaaatc
    15001 taaatgctac gctaagcccc ataactgctg ctaaacatac acctattcta gtaaacttta
    15061 ctaattgttt ctgagataac tctttttttg taattcttcc atatatatca tttgctaatg
    15121 tagccccagc tgctagataa tagctatcca atgttgatat tatagaacct aaaattccta
    15181 ttataaatat tactcttatt ccttgtggta atgtatctaa aactaaagtt atatatccag
    15241 cagctggagc catttctgga taagtagctc ttactataag tcctgtcata gttaacacca
    15301 tgtcaaaaca tatccatagt gaaaaacagg ttaataaggc tcttcttcca accttaggag
    15361 aatctgctgc tgaaaatctt tgataaaaag ttgggtctga ataagctcca aaagaatata
    15421 caattaacat caatgctttc atagctgaca taccctttgt agggtgcata agctgtggtg
    15481 catctttgag tgcttctgta agaccttgaa accctcctat ttgttcaaat tgacctggca
    15541 taaccatagt taaaccaaat accatacaaa aaaacaatac catatctgta atggcaactc
    15601 ccataagccc accaagtact gtaagtacaa cgactactcc taccattatt aatcctatta
    15661 attctttatc tattttccat gctacgttac ctatagttcc catagcagtt atttctccaa
    15721 gttgtgcaca atatataaac atcaaaattg ccccaattat agcaacatgc ttgccataaa
    15781 aactttccag taaatcagga actgttatat tggttcttat atgaatcctt ggaccaaccc
    15841 acaatgctaa aggcattctc ccaatatgtg cagctattga ccaaactgcc catgcagata
    15901 ttccatatat agccgcatac tctacactac ctactgtacc aactcctcca taccatgatg
    15961 caaccaaagt tcctactatt aaggaccatg gtaaacttcg atttcctagg taataacttt
    16021 ccatatcttt tacatttcta gaaaaataat aacctattcc taataatata aatgcaaata
    16081 taactataat cccattttcc caaaatgtta acatatctaa tctccacatt cttatatttt
    16141 aatttttaac tttaatatct aaatatgaac aactctaccc atttccataa aaataggtag
    16201 agttatataa taattataag tatttaagta atcttgttat taagcaattt tattgtttaa
    16261 acctttattt tctcttttat ttattttatt taaagcataa gtagcaaata tccctataac
    16321 tgcacatact acagataaaa taaatatata tgtatatcct gtttgtccat atttatctaa
    16381 ccaattacct gcaatagtgt aataaaacgc atctggagaa aatcctataa atgaagcaaa
    16441 tccaactact gctcctgtag tttctaaaga tatattagac tctgaaacag atgcaaagta
    16501 tatacctcta aatccaaata aaattacact tagtattatc atgtttatta cagctatcca
    16561 tataaatcct gtactcttag gaataaccaa gtaagcagct gtacttactg tcattaatat
    16621 aaatcctaca aacattactc ttatagatga accaacttta tctgttataa caccagcaac
    16681 tggagatgct cccattttta taacataagt tcttattatt gataaagcag aaactagagt
    16741 cattggcatt acataaactt ctgataagta tggacttaaa taagttaaac ttgagaatac
    16801 taaataagtt gaaaatatta taagaccaat tagccaagcc tttggcattg taactacttt
    16861 taacaatgac ttaatactta atccttcatc tgatgctcca ctatcttttt tctcttccac
    16921 taagaaaaat agaagtatac cacatattat agatgctgca gaatataacc atactacata
    16981 tttgaatcct actgttattt cagcaaattt accaaagaag taaagaccta taaaagaaat
    17041 taatgtacct gatacacctg ataacccttc ataaaaacca aatattcttc cttgctctga
    17101 ctcacttcct tgcattctaa caaccttaac agaggctgag aagaaagtta atattgttgt
    17161 aaatccccat aatacaaata ttaaaagtat catactatat gatgggaatg tagagaaata
    17221 aaatccaagt attcctgaag caattaaaga aaaagttatt aattttctaa ctgagaatct
    17281 atctgcaaac catcctccta taaaataaga taccattcct actaatccat ataaactcaa
    17341 taagttccct atttgtgtat gacttaatgc taaagcttgt tgcattgggt cataaaatga
    17401 tgatttcata tatggtaaac tatacatagc tgttgttcca aatgccaata tgaatattat
    17461 caaaaatctt ttaacattat ttttgctttc cattgattct ctctccttaa aattttattg
    17521 tttataatct cattcatata attttatttt aatttttcta ttgcttgttt aaaatcattt
    17581 attatatcat ttgaagattc taatcctaca gctattctta ttagattttc cccattcatt
    17641 tgaactaatg tatgctcttc ccctaagctt gttgctattg ttgcaagctc taaagaattc
    17701 attaatcttc tactagcttg gtctcttgtc aatccattga taccatcctt aagtttaaat
    17761 gaaactacac ctccacctaa accattcatt tgagttttac aaagctcata ttgaggatga
    17821 gattttaatg ctggatactt taccaattcc acattctctt cttcctctaa aaattctgct
    17881 actgctaatc cattttcaca atgctttttc atcctcatat ctaacgtttt catacccctt
    17941 aaaatcatcc atgcactcat tggtggtaaa gatgctcctg taaaacaaca taaacttggc
    18001 catctaacta aatctattaa ttcttttgaa cctgctactg ctccccctaa agtatcccca
    18061 tgtccattta taaacttagt aagactatgt attactaaat ctgcacctaa atctattggt
    18121 ctttgtacaa ttggtgtccc aaaagtacta tcaactatag ctaagctttt attttcatga
    18181 gcaatttctg ctattgtttt tatatctact aagtctaatg atggatttaa aggactttct
    18241 atataaacta ttttagtatt tggtctcatt gcttctttta cattttgtgg atttactgca
    18301 tctacaaatg taacttcaac tccaaattta gataacaact cagacatgaa taatttagtg
    18361 tgtgaaaata ttccatctga actaactata tggtcaccac tttttaccaa tgctaatata
    18421 gtagatgtta ttgcccccat ccctgatgta ctagatatgg cactttctgc attttcaagc
    18481 atagccaact tagcttctaa ctctgtaaga gtaggattac actctcttgt atacacatat
    18541 ccccatgtca tacacaactc ttcaaaatgc tctactgtct ttgcagcaaa tgttgctgtt
    18601 tgacatattg gtgtagccaa tgcacctgtt gttgggtctg cactatgtcc ccaatgtatt
    18661 atttttgtct ctaaatcttg attaatattg ttactcattt tgatttcctc ccattttgac
    18721 ttatttatac aacgttttcc ctaccaaatt tcatcaattt agcttagtct taatacattt
    18781 tttcaagtat acttttttat ttgttataat ttctaaaaat atgacactta ttttgttttt
    18841 ttacagttct aattataaac tctatagtaa ctatagagtc aatatgtttt tgatattttt
    18901 tttacaatgt ttaaaatagt cagtacaaaa ataggtatat cttagaataa ctaaagtcat
    18961 cctaagatat acctattttt atatcacaat ataaataatt acatataagc attagttttt
    19021 ttatcataaa tagatttttc aagatttatc atcttttcat aaatatatta tagttaaata
    19081 ttttaatatt acctaacatc tacaataaaa tatactctta aaataaaatt atataattaa
    19141 aaatactcta taaaagctaa aaagtttttc tattcgctac atctaaaaat aattagatag
    19201 aggtataaat gtatttagtt tatttatacc tctagtcttt tataaattat atctctacta
    19261 aattgatttt ttagcacgaa taagtgtgct cttggagata ccagtcatct tcacaacctc
    19321 tctatatggc ttagaattta ataaccccaa tgcatattgt atctgttgat tgctgtattt
    19381 ctttggccta ccatctttaa agtccttttt ctttttagca aattcttttc ctaattgagt
    19441 acgttctatt atcatatttc tctcaagttc tgcaactgca agtaatgtag tcaaaaagaa
    19501 tttacccatt gaagtatttt ctaaaaggcc tatatttaaa atatgaacac ttacttcttt
    19561 agcaaataaa ccttctatta gttgtattcc ttctgatgta cttctagcta gtctatccag
    19621 cttagttgca acaagtaagt ctcccttttt tagagatgat ataatttttt caaatttagg
    19681 cctgtttgta gtagtgcctg tataagtttc ctttattatt ttagcatcat tatatttatt
    19741 caaaatttct tgctgttgct gatacaaact gttgttgtct gtttgatttt ttgtacttac
    19801 acggcaatat ccatatataa taatcatcac ttcctaacat aaagattttg tacagaagtt
    19861 acccagaagc ttgtttttac ttatctttat ccattttact aaaacgattt ttgggtgtca
    19921 atactgttaa cttttgacac cataataatt cctttataat ttgtttgact atgtcaaaaa
    19981 ttaccaatat aatgaaatta atgagtttat acaaattgga ggaaataata tgaatagtct
    20041 gttaaaagaa gcaatagatg aattaaaaaa catccattgc ggggaagttt ttttattaaa
    20101 agaattgttc aaaggctatg aatggaacag gttggagatg aatatcagat tgaaattagg
    20161 aaaacttttt ttgtatgaag tggtaactaa tcccaattta aatgtagagg tcatcaataa
    20221 aaattctgct tatcagcaaa tgtataaaaa aaaatagtga cttaaatgta tataatagta
    20281 taatcttctt attgattcaa aagttaaaac ttttaataca aaacaatcat tctattttaa
    20341 catttatcaa tatgtctaaa tttatataat taacacatac gaatagtatt gtttaaatca
    20401 cattatttaa gatagataaa tcttaaccct actccagcat aaatactaaa atagggctta
    20461 gaattatcta tatttttatc ccataaaatg cttagctctt cctcttgatt gaacatatat
    20521 atctggggaa atacaataga agcgtaaatt tataaaattt ccaaagtcag aaaacttcag
    20581 ttcaaactta ggtataccaa aactttcagg tgctatttcg tcaacgccaa aatagatgat
    20641 aattccatta tctgtaagat aaaatgcttg gtcttcaggt ataactaact caacattaga
    20701 ataatacatt tctctatttt catttatttg agatttaatg taatcagtaa ttatattaat
    20761 ataatcaact ccatctctaa aaatgtcatt aagtaaaagt tcttttccag ttgtcaaatc
    20821 tatattgtaa ttattcaatg tattgtattt aggcccatag ttattagcaa accccataag
    20881 gtttaatatc atactcagta cataattttt attaaaagta actgcgtaac tggtagatac
    20941 ccaatagctt tgttttggaa gtgaattttg agaagttgcg ttattatatt ctcgttcttc
    21001 ttcctgtaag ccatttttaa aattatttac agtcattctt acagcattgt ttatgttatt
    21061 taaaataact gggtttacat acgtggattg atttctaata ttgtatataa caggatactt
    21121 tacatcatat gaaaaatact tacttctttc aaaatcttca ttaaattgta catataatat
    21181 attatatgta caggtatttg tagattgata attttccatt aaaatcctcc cccttttata
    21241 aaaaaatcag atacaatata atatgcttaa aacagtaata atgttaatgg taaatgtaaa
    21301 gaatagaaag tatctaaaac agcaaaaaat gactaatttt gtggtataat acataggatt
    21361 gagagttatt ttataggagg aaaaatatgt tattattagc agataaatgg aaagattatg
    21421 aacttataga tatgggaaat ggagaaaaat tagaacgttg gggaagttat gtacttagaa
    21481 gaccagaccc acaggttgta tggccaatgg aatctgaatg ggctttatgg aaaaaccctc
    21541 atggacatta tcacagaagc aataaaggtg gaggtcaatg ggaacacaaa aaaagatatc
    21601 cagaacaatg gactataaat tataaaaact taaagtttca cattaaacca actggcttta
    21661 aacatactgg attattccca gagcaagcag ctaactggga ttggatgata gagaaaataa
    21721 agaaagctaa cagacctata aaagtgttga atctttttgc atatacaggt ggagctactg
    21781 tagcatgtgc atctgctggt gcagaagtat gtcatgttga tgctgcaaaa ggtatggtta
    21841 attgggcaaa agaaaatata gaattatcag gtcttaaaaa tcaaacagta agatttatag
    21901 tggatgatgt tgtaaagttt gttgaaagag aaataagaag aggtaaaaaa tatgatgcaa
    21961 taataatgga ccctccttct tatggaagag gtcctaaagg agaagtatgg caaatagaag
    22021 aaaagttata cagttttgta gatttatgta tgggagtttt atcagataac cctttatttt
    22081 tcttaattaa ttcttataca acaggatttt ctccaatagt tcttgaaaat gtattgtcta
    22141 caacaatagg taaaaagaca aatacaggag atatatacgg tggggaatta ggaataccag
    22201 cttccagaga tggtaagata cttccttgtg gtatatttgg aagatgggag aaaaaataat
    22261 gataaaagtc atatatgaag ataaccattt attagttgta gaaaaaccag ttaacatatt
    22321 gtctcaagga gataatacta atgataaaga tatggtaaat cttttaaaag actatattaa
    22381 aaaaaagtat aacaaacctg gaaatgtata tattggttta atacatagat tggatagacc
    22441 agtaggtgga gttatggtgt ttgcaaaaac atcaaaagca gcatctagat tgtctgagca
    22501 ggtaagaagt aagactttca aaaaaactta tctagctgtt gttcatggca agataaaatc
    22561 taactcagat atactaaaag attttttata taaaaataaa aaaactaata tggtaactgt
    22621 agtgaaaaaa agccataaag atgccaaaga agctgaactt aactataatg tattagattt
    22681 taaagaaaat ctaagtttag ttcaagttaa tttaaagact ggtcgttctc atcaaataag
    22741 agttcaattt gcaagcagaa aacatccatt atacggagac caaagatatg gcaaagaaat
    22801 aaataagatt ggacaacaaa tatctctttg gtccaataag atagaaattt gccatcctac
    22861 tacaaaagaa aaaatggaat ttacatgtca tccacctgac aaatatccat ggaatatatt
    22921 ttaaatctca aatgaggtga ttatatgagt aaaaaatttt caataggaga aatgtctaaa
    22981 cttcataaaa taagcataca gacactaagg tactatgacc aaatagattt atttaaacct
    23041 gttcatgttg accaagaaag caattacaga tattactcaa ttgaccaatt ttcacaatta
    23101 gatattataa aatatttaaa atatttaggt atgcctttaa aagaaattaa gtcaaagctt
    23161 gatgaaaata atgaagaaat tctaaaatta ctaacaaata aaattaatat agtggatgaa
    23221 aaaattaaag aattagagct tataaagaag gtactaagca caaagaaaga aactatggaa
    23281 aaaggtatga aagtagatga agttggtaaa atagtgagaa aacatattcc acgaagaagt
    23341 atcttatcta tagagtatga agaaggatta agtagtgaag aaactatgga actttccaga
    23401 agacaaatag caaatatatt agaagataat atatctgtgt tctatggtgg agtaagtgga
    23461 cttgtatcta tagataaatt tttaaaagaa gataaagtta tttatgacaa tagttttgtt
    23521 actgttgaaa gagacttatt tgatataaaa tcacaaaaac atataacaga aatttcatct
    23581 ggtgatttta tttgtatgac atataaaggg ccttacaaag aaaattatac cgcttttaag
    23641 aaactggttg attatgttga atataaaaga atacctattg aaaattatat atatgaaata
    23701 cctataatag accctctgtc tacaaacaac gaagaggaac ttctaacaga aattcaaata
    23761 caagttaaaa agagagaaat tgacttaaga tgtctagtgt agtaatctag tttacttaca
    23821 catcttaata tagttgtcta ttatcaacat atgatttttc tagatagtga ttcctaacag
    23881 tgagattttc aaaaaatagg aggaaatatg gctaatgcta attccctcct atttttaatt
    23941 aagccatatc attttaatta agccatgtca tcacgaagtg catcaataat attttctttt
    24001 tttatcttat taatagtata caacattgta ataaatacta taaataaaac actaaatata
    24061 ctaattccaa tactaaacca tggaatcaca aaatcaatat catctgtaac cattacttta
    24121 tgaattagcc atgaagaaat tactgctata ggaagtccaa agaacaatgc tctcatgcca
    24181 taaaaggcac actcaaaatt catcatcttt tggaaatcat gctcagacat tcctacagag
    24241 cgaagcatgg caagttctct acgacgtaac ttaatattag ttgaaattgt attaaacaca
    24301 ttggcaatcg aaatcagaga aatcataatt atgaaagtat aagcaaaaac attggcaata
    24361 aaaatgtaat tacggttctc atccatcatt ccatacatat tgtacagatt gtactcattc
    24421 gtaactccct cactctcaat aatttgctcc atttctgcaa ctgactgagt aggatttttc
    24481 gattgaaagc ttaaactttc aattcctgta gaaacaccag gaatctcaaa cttatctttg
    24541 gctgaataag gagccaatac tctgaagctt gctttcttca catctgaact tctgattcct
    24601 ggaggagaat caggtggcac tactttgaca aatgaaatat tcacattttg tctctcaggt
    24661 atctccaact tgccattagt ttcaggagtg aggctaacat tcatagaaga actgttgaaa
    24721 atatcaataa tattattatc ctttgttacc atagaaacag caatcagttt tgcattttgt
    24781 ccagtatatt cttctgctgg taatccaaga cttttaataa tattcagata agttctatca
    24841 tcaagaaatt gcatatctac tggtaattca agcatttcac tcatagaatg tgaaccctca
    24901 tagttcaaat aatcgtctgt aagctcactt actttggcaa cacatgaata ggtcataagt
    24961 ttctgataca aaccttcgta aacaccattt gcagttttta gcttatcgta aagcttcaac
    25021 atttcgttgt ctttcatacc ttgtgtagaa aaaataatat cctggtcagt aacctctgac
    25081 ctctgatttg acatctgttt taggtctgct actaaagcac ttgtagatat aaatagaact
    25141 atgcttaaaa caagcgacaa tacaatactt cgataacgtt tcttgtttct tttaaagttt
    25201 ttcatagcaa gaactccctc aagaccataa actcgctgtg ttagttttga tgtcttcaca
    25261 gatttagatt caatttttac ctcatttgtc tgacgaatac actccatcac tggtgtgttg
    25321 gcagcctttc ttgctggtat gtaagcagaa attaagattg taaccatact taccactgct
    25381 gcaacgatga ttgcagggat agacaatgtt aatgtcaacg gaacgtttac atagagaata
    25441 tttgcaaagt ttttagctat gacagaaatc acaagtccaa tactgactat accgattgca
    25501 acaccaattg gtataccaat cgcaccaatg caaattcctt caaatagcac cgaatttcgt
    25561 aactgttttg ctgtagctcc aacagatgaa agtattccaa attgatgtgt tcgctcgttc
    25621 aatgacatgt taaatgaatt atatattaga aaaattgagc ctagcatgat tatggcaagc
    25681 acaatgccac caaccgagta caaaaacata ttgaacaaat tgttatcaga aacacctatg
    25741 aagcgcaaca cattgtcatt gaaaatggaa gcatggtctt tagtgctact tatgtaagag
    25801 cgaatctgat gtggattttt caatgtgaca aaaaggctaa gactgtcagt cttgacttgt
    25861 ttatcttctt tggttatcaa ggtatatcct ggtgcagaag attcttcaaa tacgggcttt
    25921 ctacagatac ccacaactgt ataagttttc ttatcctgtg gcacaaaaac ttcactccca
    25981 gcagtgtatg ggtcactctg accaagcttt tcattttcac tcattcgttt ccctacatcc
    26041 aaagaaatag tatcacctac tgcaaatttg acaccaccat ctgtcgcaac tttacttgaa
    26101 acaagaatct ctctactatt ttcaggtaat cttccagaaa ttaaagttat aggcaaagta
    26161 tcaaaagctt ctttgctaaa tccagcaata aaaagatatg gtttatctgg atttctgtta
    26221 ccttcaagtg ttgcatagcc aatattttca aatgttgcag tatttgcaac ttcatcattg
    26281 actctttgtt cctgtacaaa agaagaatcc acatccaaaa actggacatg ccaatcacca
    26341 tattttataa tagctccatt tgtcatatag ttcatcaaag aaactccaaa ggtagtaact
    26401 gctgtaatca tagcagctga cagaacaact cctataattg ttacaagagt tcgagtgcga
    26461 ttctttttca atccttgcag agcaactttg ttaaaaatgt tcattaacga ttcaccttcc
    26521 tctcatcacg cactactttt ccatctgaaa tacagataat tctatctgcc tgtaaagcaa
    26581 tgttttcatc atgtgtgaca ataatgagag tttggtgata tttactatgg ctctctttca
    26641 gtaactggat aatctcttgt ccatttttac tgtctaagct acctgtaggc tcatcagcaa
    26701 gcataactgc tggtgcattc atcaatgcac gaccaatggc aacacgttgc tgttggccac
    26761 cagaaagttg atttggtaaa tgtgtttttc gttcttttaa accaagcaat tctaacaagt
    26821 catttaaccg ttcttgattg accttacgct tatccataag tataggcaat gtgatatttt
    26881 ctactacatt taatgtgggt atcagattgt gaaactgata aatcaatcca acttgtcgcc
    26941 tacgaaaaat agcaagtttt tcattgcttt ggccatatac atcttgcccc tccagatata
    27001 tctttccact tgttggtacg tctactcctg caatggaatg tagtagtgta gatttaccag
    27061 aaccagaaga accaatgatt gcagtaaact ctcctttttc aatagtaagt gagacatggt
    27121 caagtgcagt aaccttattt tcattcttac cataaacctt acataaatct tcaatcttta
    27181 aaaacgccat tatataagtc tcctttcata ttttataaat atatcatagg actttcaact
    27241 tacatttaag tgacatttaa atgagagatt cgtcactttg gaaaacgaat aacaaatatt
    27301 gcaccacctt gtgggtgatt ttgtgcagta attgttcctc cttgtctcat aataatcgtc
    27361 ttacaaagtg ccaatcctat tccataaccg gctgtgcttg aatttttccc acgataaaat
    27421 ctgttaaata gacatggtaa atcttctttt ttaaagcctg ctccgttgtc atgaatagta
    27481 agttgagtaa acaaaaagtt gtcctcacaa atgatatcaa tttttccatt atcacccaca
    27541 ctctccatgc agtttttaaa tatattttga attgcttctg aaagccaatt taaatcaccc
    27601 aaaatcctta ctccttttgg tatatcaata tgtaaagtga tattgtgcag ttccatggga
    27661 atcaaaagtt gatgaagtgc agaactaatc aaattattca catcaatttg ttctttttga
    27721 aagactacaa tacctgcatc taaacgtgat aattttaaaa gggaagtaat cagccagtcc
    27781 attcgtataa gtaattcctc tgtttctcgt atcaactctt ttctctcatc ttcatcagaa
    27841 gtattctcta acagtgacaa aataagagtt actgatgtaa gaggagtgcg aagctggtgg
    27901 gctatgtcag ctagtgagtc agcaaggtgt tctttttctt ttttcaatgc gtaattttgc
    27961 tctctaatac gtaacgtcat tttagttatt tcactttgca gaatagaaag ttcaccctca
    28021 tctgattcag caatatacag atggttagca ttatgaagca caagattaat ttgttctgaa
    28081 atctgtgcaa tacttttgta tcgattttta gtaaacacaa aaaatgctat tccaaaggta
    28141 gtggcagaaa caatagaaag tattccagtc ataatactga ttgcaaatcc cagggctaca
    28201 gtgagaatag taattaataa gaataaaata gcaaattttc gaaactctct atttcgaaac
    28261 ataacttccc cccaatctat accctgtccc acgaacagtc aaaattattt gtggacttga
    28321 agggtcatct tctatcttct ctcgcaagcg tttgatgtac acagtcaatg tgttgtcatt
    28381 aacaaactca cctgccgcat cccacaattc atcaagtaat cttaccctcg taataatact
    28441 tttaggattg ctaataaaca ccaataacaa acggtattct aaagctgaaa gaaaaacctc
    28501 attgccattc tttttcacaa caccggttgc cgtatctaca taaagcccac aaatttcaaa
    28561 agttgaccca aaacgcccac tttttcgtag tgctgttcta attcgcgcaa tcagttcacg
    28621 tggacgaaaa ggtttagtaa tataatcatc tgcacccata ttcagtccag taacaacact
    28681 tgattcatca ccggaagctg tcaaaaagat aacaggaatg tcttgctttt ctttgatttc
    28741 tgtgcaaatc gtaaacccat ttccatcagg taaagaaata tcaataagtg ccaaatcaaa
    28801 tttattctta gcaatctcta caagtgcttc acttcttgtg gatacatggg ttactataaa
    28861 tccttctgaa ttaagcaaaa gaataagatt tttggcaatt gctttatcat cctcaactaa
    28921 aaatatccgt ttcatttact ttcatcctcc tttattttat tagttttata tattccagca
    28981 gagcaatagt acactactat tttacttatc attacacaat tttcagtagt ttcttttgtt
    29041 cttatataat ccatgcataa tacctccatt gatttattat atttcttata acataaatac
    29101 acaagataag acaaaaaaca taatgtaagt gcaagttttt taactcacta acttataaac
    29161 atacactcct actaacacta ctttcttgat gacacatctt acaagcttat attcatacat
    29221 caatgaaaaa ttaaatttat ccatttatta aaataaaagg caatagcttc tatagctaat
    29281 gccctttatc ttaaaatgat ttaaatacta tacaattttt ataaatggct ataaataaac
    29341 tcgtatagaa tataaaattt atctacagtc ttttgttata caaaatttat aacctcttat
    29401 agaacaaatt taccactttg caaacttatc acttttactc agctatatta taaactttta
    29461 atttatcttt gtttaaatgt ttcttcttaa cttctactaa agcttttagc gtatccaaag
    29521 atgtcattat ttcagtagaa aattcaatgg ctgttcttct catcttaaat ccatctcttg
    29581 tagagtcatt tcctttagtt ggtgtattta ttactatatc aacttgttta tttcttatag
    29641 catctaatat gtttggtcta ggctcttcaa ttctatttac tatatcagcc tctattccat
    29701 tttctcttaa actcttcgct gttccttctg tagcaacaaa agtatatcct aattctttca
    29761 tatcttttgc tatctctata aactcatctt tatcatgatt atttacagta gcaagtacta
    29821 ctcctctttc atcagacata tgtctacctg ctgccaagaa ccctttatat aaggcttctt
    29881 ctaaattttc tcctacacct agaacttctc ctgtagactt catttcaggt cctagactta
    29941 cttcaacttt agataattta gacattgaga atactggtac ttttactgat actaattttg
    30001 gctctttgta aactcctgta ctatatccta aatcctttaa tttagctcct aacatacact
    30061 tagtagccaa gtcaactatt ggtactccac ttactttact tatatatgga actgttctac
    30121 tagctctagg gttaacctca attatatata attcattttg aaattctata aattgtatat
    30181 taaccatacc aagcacatta aggtctaaag ctattttttt agtgtattct actatttttt
    30241 cttttatttc atcagatata ttttgacttg gatacattgt tgtactatct ccagagtgaa
    30301 ctccagctct ttctaaatgc tccattatac caggtatcaa tatatcttct ttatcacata
    30361 tagcatctac ttctatttct ctaccagtta gatatttatc tataagtact ggatttttat
    30421 ggtctctatc aaatgcatca tccaaatact gaattagttt ttcttcgtta taagttattt
    30481 ccataccttg accaccaagt acataagatg gtctaactaa tactggataa cctaactcct
    30541 tagcaatttc aacaccttca ttagttgtcc atacaccttt tccttttggt ctatttatat
    30601 ctagtctctc tagtaaatca tcaaattttt ctctatcttc tgctgcatct atatctctaa
    30661 agtctgttcc cagtataggt atattttttt catgtaaaaa cttagccaat tttatagctg
    30721 tttgtccacc aaactgtagt attacacctt ctggtttttc tttttctatt atatttaaaa
    30781 cttcttcttc tgttaatggt tcaaaataca acttatcaga tgtatcaaaa tccgtactta
    30841 cagtttcagg gttattgttt acaattatag tttctattcc catttttctt aaagatttta
    30901 cacagtgaac tgaacaatag tcaaattcta ttccttgacc tattcttatt ggaccagaac
    30961 ccaatactac aacttttctc ttatcactaa ctactacctc atcatattgt tcataagttg
    31021 aatagtaata aggtgataga gcttcaaatt ctcctccaca tgtatcaacc attttataaa
    31081 ctggttgtat attatataaa cttctcaatt catataattt ttctgggctt atcttcatta
    31141 agtcagatat acctttatca gaaaatccct ttttctttaa ttcatgtaaa tattctttat
    31201 ctaagtcttc tatttttaga acttttaact tttcttcttg ttctactatc catttaaatt
    31261 tatttataaa ccacttatca acaccagtta tttctgatat catttctact ttatagcctc
    31321 ttcttatcat ttcagctaag tcaaatagtc tctcatcatc aggtactact actctagcct
    31381 ttaattcttc taatgttcta ccttctgatg gagcatgaac caatgaatat tttcctgttt
    31441 caagtgatct tataccttta agaatagcag cctcaaagtt acttcctata ctcatgattt
    31501 caccagttgc catcatctta gttcctaatt ttctattggc atgtctaaat ttatcaaatg
    31561 gccactttgg tatttttact accacataat ctaatgttgg ctcaaaacaa gcataagttt
    31621 ttttagttac tgcattttct atctcatcca aagtataacc taaagctatc ttagctgcta
    31681 cttttgctat tgggtatcct gtagcttttg atgctagggc tgatgaccta ctaactcttg
    31741 gatttatttc tattatcgca tattctaaac tatgtgggtt taaagctatt tgaacgttac
    31801 atccaccttg aacctctatt gcatttatta tgtctattga tgcctttcta agtagttgat
    31861 actcttcatc acttagagtt tgacttggag caacaactat actgtctccc gtatgaactc
    31921 caactgggtc tacattttcc atattacata cagttataca gtttccattt ccatccctca
    31981 ttacttcgta ttctatttct ttccaacctt ttatactctt ttcaagaaga acttgtccaa
    32041 caggacttag ctgtagtcca tgtgaaagta tttctcttaa ttcttcctca gtatcagcta
    32101 tacctccacc agttccacct agcgtatatg ctggtcttac aactactgga tatcctatct
    32161 tattggcaaa ctcaagacct gcttctaaat ttgtaactat atcactaact attactggtt
    32221 gatttatttc tttcattact tctctaaata aatctctatc ttcgcccttt tttatagatt
    32281 ctatagatgt tcctataact tttacattat atttatctaa tataccagca tcatgtaatt
    32341 ctacagctaa gtttaatcct gtctgaccac ccatacctgc aagtaaacta tctggttttt
    32401 ctttttctat tattttttct ataaattcta ttgttaatgg ttctatatat attttatctg
    32461 caatttcttt atcagtcatt atagtagctg ggttactatt tactaataca acttcaattc
    32521 cctcttcttt taatgcttgg caggcctgtg ttcctgaata gtcaaactct gcagcttgac
    32581 ctattattat tggccctgac cctaatacta aagttttctt tatactatct aatttaggca
    32641 taactctttc tccttatcta aacaattttc acagttctta tcttcatcat ctatacaatc
    32701 aactttaatt actttatttt atcttaaatt caaatttact catcaataca tctatttaat
    32761 acatctatgt taattaaacc aaactttttg atttaagtta ttcttgtttt ttagttagct
    32821 attctaatgt tatttatttt actaattcta aaaatttatc aaaaatatag tcattatctt
    32881 ttggtcctgg gcaagcttca ggatggtatt gaacactgta tacatctagt ttctcatgtc
    32941 tcattccttc aacagtatta tcatttaaat ttatatgtgt cactttcatt tgttcaggaa
    33001 cttctgaaac ataataacca tgattttgag aagttataaa tacttttcct tcttctaaat
    33061 ctttaactgg atgatttcca cctctatgcc caaatttaag tttagcagtt tttcctccaa
    33121 gagctaaagc taaaagttga tgtcctaagc atattccaac tataggtttt ttaccaataa
    33181 gagcttttat attttctata acatcttcta ggtcttctgg gtctccaggt ccatttgaca
    33241 agaatattaa gtcagggttt atactcaata catcttctgg agatgttgta gctggaaata
    33301 ttgttatatc acatccacga gctataaatg atcttaatat atttcttttt actccaaaat
    33361 ccataacagc cacttttgga ccatttccct ttatatgctc tacttctttt cttgtaacag
    33421 ttctaactgc ttctgtattt gaaaacttat ccaatttatg tttaacatct tctaatttac
    33481 tgtcttctaa tgtaattata cctttcatgg ttccattatt tctaagtatc ttagtcaaag
    33541 ctcttgtatc tattccttca agtccgataa ctttattttg ctttaaatat tggtctatat
    33601 caatttcaca tctaaaatta cttggagaat cacttttctc ccttactatg aatcccttta
    33661 cttgaacttt ttcagactct gctgatgata aatttatacc ataatttcct acaagaggat
    33721 aagtcatagt aactatttga ccatagtaag aagggtctgt taaaacttca ccatatccta
    33781 tcattgaagt attaaatact acttctccta cactttcttc taaatatcca aatgcctttc
    33841 ctataaaaac tgttccatct tctaatatta accttgcttt catactacag tacctcctat
    33901 aattctcttt agacaaacta aagaacatta actaaatata agcttttaaa tatctatatc
    33961 tatttttcac ttttacattc tagttttata cttatttaca tttatttttt atctttttct
    34021 attatactac aaatttctta aaaaaaagaa gttatctcaa aagtaaaaac tcctagaaaa
    34081 aataacctct tttttttatt taataattaa attcttaaac tatacaatca acttaatttc
    34141 tattcagcta tattgtaaac ttttaactca tctttattta aatgtttctt cttaacttct
    34201 actaaagctt ttagtgtatc caaagatgtc attatttcag tagaaaattc aatagctgtt
    34261 cttcttatct taaatccatc tcttgtagag tcatttcctt tagttggtgt atttattact
    34321 atatcaactt gtttatttct tatagcatct aatatgtttg gtctagattc ttctactcta
    34381 tttactatat cagcctctat tccattttct cttaagctat tagctgttcc ttctgtagca
    34441 acaaaagtat atcctaattc tttcatatct tttgctatct ctataaactc atctttatca
    34501 tgattattta cagtagcaag tactactcct ctttcatcag acatatgtct acctgctgcc
    34561 aagaaccctt tatataaggc ttcttctaaa ttttctccta cacctaggac ttctcctgta
    34621 gacttcattt caggtcctag acttacttca actttagata atttagacat tgagaatact
    34681 ggtactttta ctgatactaa ttttggctct ttgtaaactc ctgtaccata tcctaaatcc
    34741 tttaatttag ctcctaacat acacttagta gccaagtcaa ctattggtac tccacttact
    34801 ttacttatat acggaactgt tctactagct ctagggttaa cctcaattat atataattca
    34861 ttttgaaatt ctataaactg tatattaacc ataccaagca cattaaggtc taaagctatt
    34921 tttttagtgt attctactat cttttctttt atttcatcag atatattttg acttggatac
    34981 attgttgtac tatctccaga gtgaactcca gctctttcta aatgttccat tataccaggt
    35041 atcaatatat cttccttatc acatatagca tctacttcta tttctctacc agttagatat
    35101 ttatctataa gtactggatt tttatggtct ctatcaaatg catcttgtag gtattgagat
    35161 aatttttctt cgttataagt tatttccata ccttgaccac caagtacata agatggtcta
    35221 actaatactg gataacctaa ctcattagca acttcaatac cttcgttaag tgaccataca
    35281 ccctttcctt ttggtctatt tatatctagt ctctctagta aatcatcaaa tttttctcta
    35341 tcttctgctg catctatatc atcaaaatca gttcccagta taggtatatt tttttcatgt
    35401 aaaaatttag ccaattttat agctgtttgt ccaccaaact gtagtattac accttctggt
    35461 ttttcttttt ctattatact taaaacttct tcttctgtta atggttcaaa atacagctta
    35521 tctgatgtat caaagtctgt acttacagtt tcagggttat tgtttatgat tatagtttct
    35581 atgtccattt ttcttaaaga ttttacacag tgaactgaac aatagtcaaa ttctatacct
    35641 tgacctattc ttattggacc agaacctaat actatcacct ttttcttatc actaacaact
    35701 acctcatcat attgctcata agttgaataa tagtaaggag atatagcatc tatctcacct
    35761 gcacaagtat caaccatttt atatgctggt ttgatattgt atagacttct caattcatat
    35821 aatttttctg gacttatctt cattaggtca gatatacctt tatcagagaa tccctttttc
    35881 tttaattcaa gaagatagtc tttagttaaa tcttcaattt tcataacttt taatttttct
    35941 tcttgctcta ctatccattt aaatttattt ataaaccatt tatctatacc tgtaatttgt
    36001 tctatcattt ctactttata gcctcttctt atcatttcag ctaaatcaaa tagtctctca
    36061 tcatcaggta ctacaactct tttctttaat tcctcaatgc ttctctcctc agatggtgtg
    36121 tgaactaatg aatattttcc tgtttcaagt gatcttatcc ctttaagaat agcagcctca
    36181 aaattacttc ctatactcat aatttcacca gttgccatca tcttagttcc taattttcta
    36241 tttgcttttt taaatttatc aaaaggccat tttggtattt ttgccaccac ataatctata
    36301 gttggttcaa aacaagcata agttttttta gttactgcat tttctatctc atctaaagta
    36361 taacctaaag ctatttttga tgaaacttta gctattggat atcctgtagc ttttgatgct
    36421 aaagctgatg acctactaac tcttggattt atctctataa ctgcatattc aaaactatgt
    36481 ggattaagtg caaattgaac gttacaacca ccttgaactt ctactgcatt taatatatct
    36541 aaagatgctt ttttaagcat tgcacattct ttgttactta aagtttgagt tggagcaaca
    36601 actatactgt ctcctgtatg aactccgact gggtctatat tttccatgtt acatacaact
    36661 atacaatttc cctttgaatc tcttattacc tcatattcta tttctttcca accttttata
    36721 ctcttttcaa ttaatacttg tccaacagga cttaattgta gaccatgtga aagtatttct
    36781 cttaattctt cctcattatc agctataccc ccaccagttc cacctagtgt gtatgctggt
    36841 cttacaacta ctggataacc aattgtaagt gcataatcaa gacctgcttg taaatctgta
    36901 actatatcac taactattac aggctgattt atttctttca ttacttctct aaatatatct
    36961 ctatcttcac cttttttgat agattctact gaagtaccta ttatttttac tccatattta
    37021 tctagtatgc ctttttcata aagttcaact gctaggttta gccctgtttg tccacccata
    37081 cctgcaagta aactgtctgg tctttctttt tctattattt tttctataaa ttctattgtt
    37141 aatggttcta tatatatttt atctgccact tctttatcag tcattatagt ggctggatta
    37201 ctatttatta atacaacttc aatcccttcc tctttcaaag actggcaagc ttgtgttcct
    37261 gaatagtcaa actctgcagc ttgacctatt attattggtc ctgaccctaa tactaaagtt
    37321 ttctttatac tatctaattt aggcataaat ttttctcctt atctaaatgg tttttactat
    37381 ctgtaaataa tactaaatct atattaatgc taagaattta ttaaatatac aatctaaatc
    37441 ttcttttgtt gtgtattctg gaatgtactg aacactatat attggtaatt ccttatgtct
    37501 cattccttct atagtattgt catttaaatt tatgtgagtt acttctgtat tttctggcat
    37561 ttctgaaaca ttatatccat gattttgaga agttataaat acctttccag tttctaaaca
    37621 tttaactggg tgattacctc ctctatgtcc aaatacaagc tttgaagtct ttcctcctaa
    37681 tgccaatgct aaaatttgat gtcctaaacc tattcctgtc aatggctttt tgcctattaa
    37741 tttttttata ttttcaacta tatcatttat atcctctgga tttcctgggc cattagataa
    37801 taagattaag tcaggattta tatttaaaat ttcttctact gtagtcattg ctggtactat
    37861 agttatatca cagttacaag ccttaagaga ctctagtaca ctatttttaa caccaaagtc
    37921 tataactaca actttagctc cagtaccttt tatattttca atttcttttc ttgtaacatt
    37981 ttttactgaa tctttgttac aatgctcttc taatttagac tgaacatctt gtaatgatac
    38041 attttctaaa gttataattc ctctcttagt tccattgttt ctaataattt tagttaatgc
    38101 tcttgtatct attccttcaa gtcctattac cttattttgc tttagatatg tgtctatatc
    38161 catttcacat ctaaaattat ttgggttatc acttttttct cttacaataa gtccttttac
    38221 ttgcacctta tttgattcaa catcttctaa atttattcca tagtttccta taagtggata
    38281 agtcatagta actatttgtc cataataagt tgcatctgtc aatagttctc catacccagc
    38341 cattgaagta ttaaatacaa cttctccaat agcttccttt agatatccaa atgcttttcc
    38401 ttcaaatact gttccatctt ctaaaattaa tttccccttc atgtaagcta cctcccaaat
    38461 attttatcta agcattttct atttctctta caatattaat agctgattct cttggatttt
    38521 ctgcttttgt tataggtctt ccaacaacta aataatgagc accttttttt attgcatctg
    38581 atggagttac aactcttttt tggtctccaa cctctgctga tttaggtctt attcctgggc
    38641 aaactgttat aaaatcagtt ccacatgctt ctactatttt atctacttct tgcgctgaac
    38701 aaacaacccc atctattcct gattcttttg ccagtttagc tctctttata gctaactctt
    38761 ctgttgtcat attacatcct atgtcctgta aatcttggtt gcttaatgaa gttaaaactg
    38821 ttactccaac tactaatggc ttttctatat ttaatctttc tgcttcttcc ctagcaatta
    38881 gtgcacattg tctcattcct tccatatctg atacatgtat tgtcattaac caaacattat
    38941 ctctcactgc agctttcact gcacttttca tagtatttgg tatatcatgg aatttaaggt
    39001 ctaaaaatat ttttttacct ttttctttaa gataatctat tgttttacct tttgtggcaa
    39061 cgtattgctc tagccctacc ttaaatatgt ctacactatc ttctaatctg tctattagtt
    39121 ctttagcttt atcaaattcg tcagtatcaa ttgcaactat caatttttct ttaccattta
    39181 tcatttttac atctcctttt taataaagcc taaataatca taaaaaaatc ctcacaccaa
    39241 aagaggcata aggattttat ctatacataa ctctattcat tagctatgag atatctttcc
    39301 cagtctctct ggactagttt aaaagacttt attatattta aatttttatt ttatactcat
    39361 caaaaacctc tagaagattt actagaggtt atatactaac attatctata accctttagt
    39421 ctctctggac tactttaagg aatttagctg taatattttt ctttatttat ataactttat
    39481 gtatctatag gataatttaa agttttatct tttttataat taaaccacta attgcttttt
    39541 atgtcaataa gtctttgaat tattcttcat ctttatcaaa caattttttc tcaaaatatg
    39601 attctgcatt atatattgca gcaactggat tatgcccagt tactctatct ttgacaacta
    39661 aagttgttat aggagcttcc atatatttca tggctaaagt atcgtgacca acacataatc
    39721 caagaattac attaaggtca accttagctt catttaaaaa taaagcttgt cctattggat
    39781 tgcacattac ctcatctgaa cactttgata ttgtttgaga tgcttctagt ccaattgtac
    39841 tcttaggcat tgccccattt ttacatataa cagaaaatac ttcaaaccca tgatgttcaa
    39901 atattttttg cactgtttgc atttctcttt ttagtccaat acaaaatgct atgccaatct
    39961 ttttataacc acacttttta gcaaactcta taatctcttt tagtctagta tattgacaat
    40021 acccctctgc ttcaactaat ccagcattat aagctatttt tctattctct tcttcttcat
    40081 aaagttgctt tgctttttgt tgtagttcaa tttctctagt tggacatatc ttcattaatt
    40141 tatccatatt tccattagaa cagcctctaa gattacattt tccacactta tacatatcca
    40201 atatcccctt ccaaaatttt attcatcttg cgattcttct ttttctagag ttacattttc
    40261 ttcttgggtt tcttcttctg gtttcacatg ctcgactaca gttataaaat atgattttcc
    40321 tattttattc cttataggaa gtttttcata tgaaacatct attttatttt ttatacaagc
    40381 tatatcaaaa tgtcttaagg ctacacctaa gctaacctta ttcatatctt cacagtgttt
    40441 cttaggtctc ttttcaaata aatgaagacc tcgttcatcc ttatggaaat accacggttg
    40501 agaatttttt acagatggag ctcttcttgt caattctaag atatctttcc actttctatt
    40561 cctatttatc ttatttatca tatcttttac tggctttcta tcaggtctag catatctcgt
    40621 cctaaacaac tcttcatttt ttgctggata tccaaatgct attattatat aaggattttc
    40681 tattttgttt tcatcatctt cttctgtttt tattccagct agataatcta aatcatcttc
    40741 ctcatcttct tcaaaattct tattcccaat atctacaaat tccaagatat cttctctctt
    40801 taagtttcct tccaaccagc aagtagctat tcccaatgtt gtaaggcgca aaactacacc
    40861 ttctgtagca aatcctatat tctgtaaata gtcctcacct ttgtctgatg taactactat
    40921 ataatgaggt gcctttactt tacattcctt acccatcaaa aaatgtatta tatgacctct
    40981 atcaacaaca tgtgctttaa tatttaaatc ttcatttaag tattctaaat ttttacaact
    41041 tctttttatt tcttccatca atggcttttt tatcttctta ctggaaaaat ttcttacaga
    41101 ttttctataa aaaatggctt cataaagctc catttatgcc ctcctaactg aaatcttctg
    41161 acgtataaac tctataaatt tatataaata ctttatattt tttatagttt tatttccttt
    41221 ttcctcctgc cctattttga aattcataag aatatctact ctagtttaat atagcattca
    41281 aaaggcttaa attctacctt atcaatgtct atacattagc ataatttttt tatttgattt
    41341 tctatgctaa tttaaataac aacactagct attacctttt taattaagct ttgccagaaa
    41401 aatttttttt catttataaa tatctatctc attattatat tataagtttt tttcggtatt
    41461 cttgtatagc ttttcactaa aaaagtattt ttatttctac tttaatatta ttatcaacaa
    41521 aaaacatact gcttaaataa gtttgttatc ctcataattt gttttaatta aaatattttt
    41581 tttatttctc aatctataaa gtataaatat tttgaatatg acccaaaatc taagtaaagt
    41641 tataatattt tataagttgt tcaatattaa aaacataagt aatgttataa tactttataa
    41701 gttattcaat attaaaaata taggaggata aatatgaatt taaatagaaa tttagaaaaa
    41761 tacgctgaat tagcaataaa agttggagtt aacatacagc caggtcaaat tctacttata
    41821 aagtcaccaa tagaatgtgc tgattttgca agaaatgcac ttaaagaagc atataaatgt
    41881 ggtgctaaaa atgtttatat agagtggtct gatgaagaaa gtactcttat taaatactta
    41941 tatgctccag atgaagcatt tcatgagttc cccaaatgga cagcagaaca atatgtagat
    42001 atagcaaaag aaggtggggc atttttatca gtctatgccc aaaatccaga tttattaaaa
    42061 gatgttgacc ctgaaagagt tgcaaatttt cagaaggcat ctggaaaagc tttaaaagaa
    42121 tggcgttctt acactctaac tgataaatgt aaatggaata tagtctctgt gccaacaaaa
    42181 gattgggcaa aaaaagtgtt tccaaattta tcagaagata aagcaataga aaaactgtgg
    42241 gatgctatat ttaaatgttc aagagtagat ggtcaggacc ctataaaagc atgggaagaa
    42301 cataatgaaa atcttaaatc taaaatggac tttctaaata aaaataactt taaaacttta
    42361 aaatataaat cctcaaaaac tgatttgact ctagacttgc caaagggtca tgtttggcta
    42421 agtggagcat ccaaagaccc taatggaata agttttaatc cgaatattcc aactgaagag
    42481 atttttggaa tgccacataa gtttaaagta aatggaactg tttatagtac aaaacctctt
    42541 gtttatggtg gaaacataat tgacaatttc ttcttaacat ttaaagatgg taaaataata
    42601 gatttctctg ctgaaaaggg actagataca ctagaaaaac ttattgaaac ggatgaaggc
    42661 tctcattatt taggagaagt cgctcttgtt ccatacaact caccaatatc caatacggat
    42721 ataatttttt ataatactct atatgatgaa aatgcttctt gccactttgc aattggttct
    42781 gcttataaga cttgtctgga aggtggaaat aatttaaaag atgaagaatt ggatgaacat
    42841 ggtgtaaatg acagtttaac acatgtagat tttatgattg gttctgctga tatggatatt
    42901 attggtgaga cttatgatgg aaagcaaata cagattttta aaaatggtaa ttgggctttt
    42961 taacatatga ttttaactaa aaagggctga tggaagttat accagacttc ccagcccttt
    43021 ttaatgttga tgataaatat tttttatatt aattaatctc attattatag attaattcct
    43081 attgttttaa acataagtat ctaaaactta tatttatatt atatataaaa tatcctattt
    43141 agtaaaacct ttattttaat ttatatgcta aaaatttaac tttgctattt caacagcaga
    43201 ctttgcatcc tttgaataat aatcagcacc catttcttca gcgtattctt ctgtcaaaac
    43261 tgcacctcct acaaatacct ttgcatttat ctcattatct ctcagtgcct gaattgtatc
    43321 tttcatactt tgaacagtcg tagtcataag tgcacttaat cctactaatt ttatatttct
    43381 ctttttagca acttccacta cttcttcaat agggacatct ttacctaaat ctataacttc
    43441 atatccataa ttttctagca ttatttttac tatatttttc ccaatatcat ggatatcacc
    43501 ttttactgta gcaactatta tttttccttt ggatacatta ttgctacttt tacttaataa
    43561 agtttccttt attgtattta atgaaacttt taccgtttct gctgattgaa tcaattgtgg
    43621 taaaaatata tctccactat catatctttt tcctacttta tcaagagcag gaattaaaat
    43681 ctcatctaat atataatttt catccttctc ttttaaaaga ttcagtgtta cattttttgc
    43741 ttcatctttt aatcctcttt caactaaaat atccaagggc aaatctttat caatctttgt
    43801 agatgaatcc gatttttttg aattatttat attgctatat tgatttatat agtttataca
    43861 tcctttatct gtgttattta atattttaaa agaattgata gcttccatca tactatattc
    43921 atttggattt ataattgcta aatcaagccc tgcccctaaa gctaatgtta gaaaagaagc
    43981 attaagtgct ttcctatttg gaagaccaaa tgaaatattt gaaacaccta atatagtttt
    44041 tactcctagt gtctttacca ttgttatagc ttttatagtt tctattgctt cttcttgttg
    44101 tgctgaaaca gttaatgata aacagtctat aaatatatct tttggtttta tcccataaga
    44161 aactgctcta ttaactatcc tttttgctat ttcaaatctt tcttcagcct ttttaggtat
    44221 ccccttttca tcaagtgtaa gtccaaccac acatgaacca tactttttaa ctattggtaa
    44281 tatactttct aaagactcct ctttgccatt tacagagttt actattgttc taccattata
    44341 atatctaagt ccttgctcta aggcctctac attggaagaa tcaacttgta aaggtgtatc
    44401 tactactgct tgaatctctc ttattaattt tggcattagt tttttttcat caatctctgg
    44461 aagaccaaca tttactccaa gtatttgtgc tccagcattt acttgctcta aacctaaatt
    44521 tatagcataa tcaacatttt cattttttaa tgcttcttgt agagattttc tacctgtggg
    44581 attcaatctt tctccaacaa ctgtaggcga ttgtacttcc acaaattttg atggtgaaca
    44641 gactacacat ttatcaattt tctcgataca tccctttgta acagaattta tattctcaga
    44701 tatttctttt ataaaacttg gattcgtacc acaacatcca cctattatag tagcaccaac
    44761 ttcaacgaac tttttaaccc cttcaaaaaa ttcttttgca tcgacatcat atatagcctc
    44821 tccatcaact atatttggaa gacctgcatt tgcttgaacc ataacaggga ctgtagctct
    44881 tgatgctatt ttttcaacta ttggtaaaag ttgtttaggt cctaatgaac aattaactcc
    44941 tattgcatct acacctagac cttctatagt agccaccata cattctggca tacatccagt
    45001 aaaacttctt ccaccttcat caaatgtcat agtacagaaa ataggtaaat cagagttttc
    45061 ttttgctgct aatacagcta ctttagcttc atataaatcc atcatagtct ctattactat
    45121 tacatcaact ccagatttaa cgccttgtag tacttgtctt ttaaatattt catatgcttt
    45181 atcaaaactt aaagtaccca taggctctag catttctcct ataggaccaa tatctaaagc
    45241 tacatatctt ggtttagtat tatctacatt ttctatagct tttctagcca ccaaaacagc
    45301 attgtcaatc acttcttcta cagtatactt actatctaat tttagttcat tacatccaaa
    45361 agtatttgta agaattacat tcgaacctgc ctctaagtat gctgtatgta tttctataag
    45421 tttatctgga ttttgaagac caaatacttc tggattttca cctaacttta accctttttg
    45481 ttgaagcatt gttcccattg caccatcaaa tattaatata ttatttttca gatattgtct
    45541 tatttccaca agagttcgct ccctttctat atgtacagtt tctatagttt ttacaatttt
    45601 cacacgactt ttctttctta gatttattta ttcttgtatt agaaatacct ataatagcca
    45661 ctactgactt tctcggtatc ataattccat tagaagtaat tgttagacct atttgttttt
    45721 gagcattcaa aacgtttaat atatctatat ttttttctat tggtaaatct ccatatcctg
    45781 ggctatatcg catagttata aatttatctt gctttaaaaa aaccttttct aaattatttt
    45841 gaacaatgtc acacacttct tctattgctg ttgttccaca tgaatcaatt attagtcctt
    45901 ttgttaaatt tgaataagag tgtcgtctta tttctttttc tatatctatc cctatagtag
    45961 ctgccataat tacacactca ttacattctt ttaaaagttc atatacatat ttactctcta
    46021 atgttaaatt tgttccctct aattctacaa cattatcttt atttattcta ataggataaa
    46081 tcctagataa atatctagga tttattttat ttttagttat agctacacat tcatcaatag
    46141 tatatttaag gtctttatct atctcttgac ctctgtattg taaatatctt aaaacttcac
    46201 tttgatttat gcttatatct tctcttccaa ttaaattatt ctccatatat atttaaagaa
    46261 aatcctttat ctaaaacatc ttttgcatat tcctttgccc caaataaaga taagtctctg
    46321 tagtttccac attctatagc tgtagctgct ggcattttat ctgattcaag aacttttttt
    46381 aatgcttctt ctaaaccaat cttaactgtt ttggcatcta catctcccca tagtattaaa
    46441 taaaatcctg ttctacatcc cattggagat aagtctatta caccatctaa tgtatctctt
    46501 aaataagtag ctaaaagatg ctctaaaccg tgcatagcag cagttccaaa actttctaca
    46561 tttggctgta aaaatctcaa gtcaaattta gttactttat ctccctttac tccatcaagt
    46621 acactacact ttcttacaaa aggagctttt acttttgtat ggtctagctt aaaactttct
    46681 actttttcca ttatttaatc ctccattata ctttaaaatt tgttacatta attctaaaaa
    46741 aaactcttcc tatctattat tgataagaag agtatattat ttcaagtcat ctcatcgttg
    46801 ttagcaggac cacctgataa aaggttggta taggatcact gagctaactc tctcccctaa
    46861 ctcttgataa gtatcttatt aaactgactt aaattatata ttactttttt atttttgtaa
    46921 acactttatt gttataatat ttatatcaca taatcgtact tttattttat aactaagctg
    46981 attgtattat tggcagttgt acttcaaaca cagtttgata tttatcgctt gaaacagtaa
    47041 tttctccatc atgtaaattt acaatatttt ttgtaatcgc aagacccaat ccagaacctc
    47101 caacatttct atttctcgat ttttcaactc tataaaatct atcaaatata tttggtaaat
    47161 ctaacactgg aattggttcc ccataattta tcacttttac aaccgccata tcttcctttt
    47221 tctctgtgac tatatctaca taatgcccat cttttccata tttcacagca ttagttataa
    47281 gattttcaaa tgctcttacc aatttatttg gatcagcttt tacaattaat ttttcttcat
    47341 taaaattaat tctactaacc ataaattctt gtttaaaata aatttcaaat tgagatacta
    47401 cttgacttaa taactctact aaatttattt ctattttgtt taattttatt gtattatttt
    47461 gcattttagt caattcaaac aaatcattta taagaacatt taaatttttc gatttttcat
    47521 atgcaatatc aacatagtac atcatctgaa cttcatcttt atacttacct tcttctatca
    47581 ctcctaaata acccattatg gatgtcaaag gtgttcttaa gtcatgtgac acatttgtta
    47641 ttaaatctgt ttttgtctgc tgagcttgtc tctcttcaat agttatattt ctaagttgat
    47701 ggactatatt attgatttta tgtgctagtt catcaatatc atccttttct ttatccttta
    47761 atatatctaa atcatactca atatctatac ttttttctaa gttaccttca tccattaaat
    47821 ttacagcact tttaatgatt tctaattttt gattttttct attagtcttt cttataaaat
    47881 ctacaatttg cttatttctc ttgtacgata taatggaata aattgctaat gatgtgattg
    47941 taaataaaaa tggataaaat aaatcatacc ctataagact tctgaaatct cttgcttgat
    48001 atacaagttg attaactaca gacatatcac tattgacata taatgatttt attatataca
    48061 tacttgttaa aataagcatt gaagttaaag cacagcttat tataagatct aatatgggtc
    48121 gaaacatatg atttaataaa cttttatttt tcaattttat atcctactcc ccatacagta
    48181 tgaattattt tttctccatc tgtatatttt tcaattttat cccttattct gctcatatga
    48241 accattacag tattattaga ttggtaatat ttctctttcc ataccttttc aaatatttct
    48301 tctgtactat atactctgcc tttattattt gctagtaaat ataatatatc aaattctctt
    48361 gatgtcaaaa ttatttcatt tccagccact gttacactat gtttttcctt atctataatt
    48421 actactccaa cctttatgac atctttatct tcattattag caccactaaa ataatatgct
    48481 cttctaatca gcgacttcac tctcacagta agttcaagtg ggttaaatgg ttttgtcaaa
    48541 tagtcatctg caccagtcat tatcccttgt attttatcca tgtcttctga ttttgcactt
    48601 aacataagta ttggtatatt caaactttct ctaacttttc tacaaacttc tatcccatcc
    48661 attttaggca tcattatatc caatatcatt aaatgaatat tttccttttt taaaatatct
    48721 agagcctctt ttccatcttc agctttaatt acattatatc cactatttaa aagatatatc
    48781 tctataaggt ctcttatttc cttttcatca tccacaacaa gaactgtatt attcattttt
    48841 acacatccta acttaatctt tgtatatagc tttattcata taaaattata ctatatttta
    48901 gttagtgtaa tctataaatt tattaatttc taaatttcca atataataca aattatttac
    48961 acatttatat tatttatttt gtaaatattc ttctaaagtt aatccttttg aataaatttc
    49021 ctcagcagca tccttaccaa catatctaat atgccatggt tcatatgctg tacctgttat
    49081 atcttcttta ccttcaggat atcttattat aaatccaaac ttgtgagcat tttctgccaa
    49141 ccatttagct tcttttgtac ttccaacaaa ccatctttct ggatttgtta aatcaataca
    49201 aagtccagtt tggtgctcac tgtaaccagg ctttgctaca tatgcatctg ccttttctcg
    49261 cccctgagat tttacccttc ttgtataagt atccagttga gtatcataag atctgtatgc
    49321 tgaagaccct aaaaactgta ttccttctga attagcttgc ttaactaaat cttcaactgc
    49381 ctttgcaggt tctcctgcca tttgtttttc ttcctctgtt gcttcttcaa caaaaggtat
    49441 gtttacctta gtaatatttt caggagtata gtttttgcta attccatttg tcttgtttac
    49501 caataatata tttgatatac tatttgagtg atttttattt tgagaatttg cctcaacatc
    49561 ttgttctgaa tcatgttgag cagctactat tggatcttta tctttcttaa gtaatctatc
    49621 tttagatata aatacactta taaatactac taaaataatg gctcctatta atttaaattt
    49681 tttcataatt aaatcttctc ctttttgtta tgtttgctat aatgttaatt ttagagtata
    49741 agacttaagt attttataag taaatcttaa ataaatctta aatttaatat ttttaaagga
    49801 tggtgataaa atgtctatac aaaaacttct aagtaaagct cgtaaggcta tacaagattt
    49861 tgatatgata caagaaaatg ataaaattgc ggttggactt tctggtggaa aagatagttt
    49921 aactctactt catatactta aaagttatca aagattttct ccacaaaact ttgaattgat
    49981 agccataaca ttaaatactg gaggtgttga taattctcca ctagataaat tgtgtaaaga
    50041 aataaatgtt cctttttatg aatttcaaac tgacataaaa gaaattgtat ttgatataag
    50101 acaagaaaag aatccttgtg cattgtgtgc taatttaaga agaggtgctt tgaacgataa
    50161 tgctaaaaaa cttggatgta ataaagtagc tcttggtcat cataaagatg atgcaattga
    50221 gacattttta atgtctatgt tttatgaggg tagagtaaat tgtttttccc caaagacata
    50281 cctagacaga caggatttaa cagttataag acctatggtt tacatagaag agtatatgac
    50341 taagaaaatt tcaaaagatt ctaactatcc tattatcaca aatccctgcc ctgcaaatgg
    50401 tcatactagg agagagtaca taaaaaatct tattgctaat ttaaataaag agatgcctga
    50461 ttttaaaaga aatgtatttg gtgctttaaa taattctgag aaattgttta tatgggataa
    50521 agagaagata aaaaatttta aataataatt aaaattttta aataaaaaag caagattgta
    50581 ttaattgtaa ataatcttta tataatctac acaatcttac ttttttattt attatatgtt
    50641 attatcttta atctatattt taatagttct taatagtatt tattttgtct aatacattaa
    50701 ttataactta aatattttat atattaattt aatctattaa ttcattcaat ct
//
LOCUS       DAEMGG010000014        46707 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEMGG010000014 DAEMGG010000000
VERSION     DAEMGG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.014"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(137..898)
                     /locus_tag="KPK24_000826"
     CDS             complement(137..898)
                     /locus_tag="KPK24_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2298533.1"
                     /translation="MEYTVQKLSKLACISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            1117..1593
                     /locus_tag="KPK24_000827"
     CDS             1117..1593
                     /locus_tag="KPK24_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG2298534.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1691..1843
                     /locus_tag="KPK24_000828"
     CDS             1691..1843
                     /locus_tag="KPK24_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG2298535.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(2003..2191)
                     /locus_tag="KPK24_000829"
     CDS             complement(2003..2191)
                     /locus_tag="KPK24_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG2298536.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            2552..2902
                     /locus_tag="KPK24_000830"
     CDS             2552..2902
                     /locus_tag="KPK24_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG2298537.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            2921..4564
                     /locus_tag="KPK24_000831"
     CDS             2921..4564
                     /locus_tag="KPK24_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG2298538.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            4645..6702
                     /gene="recG"
                     /locus_tag="KPK24_000832"
     CDS             4645..6702
                     /gene="recG"
                     /locus_tag="KPK24_000832"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG2298539.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            6761..7315
                     /gene="rsmD"
                     /locus_tag="KPK24_000833"
     CDS             6761..7315
                     /gene="rsmD"
                     /locus_tag="KPK24_000833"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG2298540.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            7317..7814
                     /gene="coaD"
                     /locus_tag="KPK24_000834"
     CDS             7317..7814
                     /gene="coaD"
                     /locus_tag="KPK24_000834"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG2298541.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            7818..8342
                     /locus_tag="KPK24_000835"
     CDS             7818..8342
                     /locus_tag="KPK24_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298542.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            8686..10605
                     /locus_tag="KPK24_000836"
     CDS             8686..10605
                     /locus_tag="KPK24_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2298543.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSIIELINKQN"
     gene            10618..10905
                     /locus_tag="KPK24_000837"
     CDS             10618..10905
                     /locus_tag="KPK24_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2298544.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            11673..11786
                     /locus_tag="KPK24_000838"
     CDS             11673..11786
                     /locus_tag="KPK24_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2298545.1"
                     /translation="MIMGRHLWNPSYFVATVLENTEEQIGKYINNQKKQVK"
     gene            11788..12906
                     /locus_tag="KPK24_000839"
     CDS             11788..12906
                     /locus_tag="KPK24_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2298546.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNVLKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQGRILNAAISKEPSGR
                     YYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNSKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            13057..14274
                     /locus_tag="KPK24_000840"
     CDS             13057..14274
                     /locus_tag="KPK24_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG2298547.1"
                     /translation="MLIGLIAAFGLVLQKKKASDVIKGSLLAAFGIIIMETGTGMLVS
                     SIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAIHLMIARFTPIKTIF
                     LTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPWLLRKYVFDVIGDDS
                     FTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREISISGGMAVFLMFIVV
                     GIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQIMPAFQGISEKVVPN
                     ALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILIYNYFNVFNLLLIPLVITSFFECG
                     AAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNWILIFGGNDLSLFGI
                     ISRFIGSLFGGIL"
     gene            14277..15017
                     /locus_tag="KPK24_000841"
     CDS             14277..15017
                     /locus_tag="KPK24_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2298548.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            15340..16650
                     /locus_tag="KPK24_000842"
     CDS             15340..16650
                     /locus_tag="KPK24_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG2298549.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            16748..17665
                     /locus_tag="KPK24_000843"
     CDS             16748..17665
                     /locus_tag="KPK24_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG2298550.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            17681..18490
                     /locus_tag="KPK24_000844"
     CDS             17681..18490
                     /locus_tag="KPK24_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG2298551.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDTMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            18548..20296
                     /locus_tag="KPK24_000845"
     CDS             18548..20296
                     /locus_tag="KPK24_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG2298552.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVSTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            20558..21580
                     /locus_tag="KPK24_000846"
     CDS             20558..21580
                     /locus_tag="KPK24_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG2298553.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            21585..23891
                     /locus_tag="KPK24_000847"
     CDS             21585..23891
                     /locus_tag="KPK24_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2298554.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            23863..24978
                     /locus_tag="KPK24_000848"
     CDS             23863..24978
                     /locus_tag="KPK24_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298555.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSINKKDKDGVPYTLVSPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            24996..26414
                     /locus_tag="KPK24_000849"
     CDS             24996..26414
                     /locus_tag="KPK24_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG2298556.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPNIILPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            26429..27637
                     /locus_tag="KPK24_000850"
     CDS             26429..27637
                     /locus_tag="KPK24_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298557.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(27711..29099)
                     /locus_tag="KPK24_000851"
     CDS             complement(27711..29099)
                     /locus_tag="KPK24_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG2298558.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            29440..30774
                     /locus_tag="KPK24_000852"
     CDS             29440..30774
                     /locus_tag="KPK24_000852"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG2298559.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(30916..31833)
                     /locus_tag="KPK24_000853"
     CDS             complement(30916..31833)
                     /locus_tag="KPK24_000853"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG2298560.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            32041..32925
                     /locus_tag="KPK24_000854"
     CDS             32041..32925
                     /locus_tag="KPK24_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG2298561.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            33138..34979
                     /locus_tag="KPK24_000855"
     CDS             33138..34979
                     /locus_tag="KPK24_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HBG2298562.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            35284..35991
                     /locus_tag="KPK24_000856"
     CDS             35284..35991
                     /locus_tag="KPK24_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2298563.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            35985..37295
                     /locus_tag="KPK24_000857"
     CDS             35985..37295
                     /locus_tag="KPK24_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2298564.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            37412..38095
                     /locus_tag="KPK24_000858"
     CDS             37412..38095
                     /locus_tag="KPK24_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298565.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            38088..40475
                     /locus_tag="KPK24_000859"
     CDS             38088..40475
                     /locus_tag="KPK24_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2298566.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAIYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            40755..41897
                     /locus_tag="KPK24_000860"
     CDS             40755..41897
                     /locus_tag="KPK24_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG2298567.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            41916..42440
                     /locus_tag="KPK24_000861"
     CDS             41916..42440
                     /locus_tag="KPK24_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2298568.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            42540..43358
                     /locus_tag="KPK24_000862"
     CDS             42540..43358
                     /locus_tag="KPK24_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2298569.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSQLFAKKLEESLNPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            43436..43747
                     /locus_tag="KPK24_000863"
     CDS             43436..43747
                     /locus_tag="KPK24_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG2298570.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(43874..45877)
                     /locus_tag="KPK24_000864"
     CDS             complement(43874..45877)
                     /locus_tag="KPK24_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG2298571.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYENMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITYPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDSSI"
     gene            complement(46030..46668)
                     /gene="recX"
                     /locus_tag="KPK24_000865"
     CDS             complement(46030..46668)
                     /gene="recX"
                     /locus_tag="KPK24_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG2298572.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        18281 a         5079 c         8008 g        15339 t
ORIGIN      
        1 aagattctgt ctcaaaatag agagagaaac ctgtttttca ttgataaaat gataaaaata
       61 tatctgattt catacaaaaa agagtgtctc atgaactaaa aagttcattt tgagacacca
      121 cctttattat tttatattat ttatctcttg tatatattac tatagcatcc cttaaaaact
      181 tagctaatcc aggttgttct ctatcataat atgatgtaaa acgttcatca tatacataca
      241 tctgtgctag agaggaatga gcttctttag agtatgtgtt ccaagtgtaa ctcaaccatt
      301 tatgatgtaa ttttgcaact tcctgagcca gttcactaga ggcatcacca gttttaaaag
      361 cttttttaag tttactaatt atttcgatat ttaggttttg ccaattttca taatcttgct
      421 tagacatatt tttaagtttt ttatttgatg tatctataat atctttacca tatttctctc
      481 taatttcagt tccataattt ttttcatttt catcaatcat cttttcttta aaaccttcaa
      541 atttttctat atctgtcata gtgtattttc cctccttcag agctatagtt ttagttacat
      601 tttctattag taaatcaatt tgctttcttt tggctagtaa tttattatgg tgttctttta
      661 acgcacttaa actatcgaag tttggtgaat ttataatgtt ttttatattt tctaagttaa
      721 ttcctaattc tctataaaaa agtatttgtt gaagtttatt aacttcattt tgaccatata
      781 tacgataacc tgaagaattt attttaagtg gtttaagaag acctatttca tcataatatc
      841 taagtgttct agtacttata caagctagtt tacttaattt ttgaactgta tattccataa
      901 gtagtacctc ctataaatta aatatacact tttacgcaac gtaaatgtca atacattaaa
      961 atataaaaat ttatatcttt taataaaaat ataaaaaaat atctttactg gatattttac
     1021 acacaactgt caatactcac taatgaaaat taaaaaataa aatttataga ctatctttac
     1081 taaaaaatag agggtctata gtctaggagg aactgtatga atttaaaaag aagcaatgaa
     1141 aaacgagaag agtacagaag aatgtattca gataaagaaa gttttttaag tttgattcaa
     1201 aattttacaa gtgtctcaat agcaaaagaa ttaactctta aaaatataga gttagaaaca
     1261 tcttttatat gtgaatataa aggaaaagaa gttgatataa tttataaggt attttctaaa
     1321 agtggcaaag tatcacatta tatagtgtta gaatttcaaa cagaaatgga tactgaaatt
     1381 gtcccaagat tgaaatcgta tagagaacaa atttggaaaa gttttattat gaaaaaaagc
     1441 cttgaagaaa tagaagataa aaattttaaa cttcctaaag ttataccagt tgttttatat
     1501 agtggacctg aaaggttaag tagaaaaaaa ggaattttgg atattgtaga atctatatcg
     1561 gagttcagta ataattctag tatgaaaaat taaaattaca taatgtgttt attggtctaa
     1621 aatggaaaac tgtcgcataa acttgaatat gaaaatattt atagatacag gttctatgtg
     1681 gaggtgttcc atgagaaata acaagtctaa ggtattgcta ggaatacttt gtctagcaac
     1741 aggaatggct gtaattctgt caatgttgct tcctggctgg atttggtcag cacttaccgc
     1801 attaatactt attggatgtg gagcattatt atttttttgt taaaatagtt ttaactataa
     1861 agtgtggggt gagtattgat gaaagtagta acaataaaat taccaaaatg gatttcaagt
     1921 ataatcctaa aatttatgag aaagagtaaa aatgattaaa ttaaaaagcc tacttacgga
     1981 agtaggcttt taaacgtggt tactaagctc tttcaatttt accagaacgt aaacatctag
     2041 tacaaacttt tactctctta ggagctccat ctataatcgc tcttacgctt cttaagttag
     2101 ctgaccatgt tcttttatta tgtttatttg agtgtgatac ttggtttcca gaaaccttac
     2161 ctttaccaca tacgctacat acttttgcca tctacgcaca cctccttaaa atgataacta
     2221 tttaaagact aaaaacatat ttatattatc ataaataaca aaaatttgca atagaaatat
     2281 atatttgcct tacacatatt gaagaatttc ttattatcat ataaaattat atatataatt
     2341 ttatataaag taaatatatt tggaaaataa ttattatttt tctttgaata tatatactat
     2401 agaagaaatt ttatccaaaa acttttctag gtttaaatta taccatattt atagtatgat
     2461 atgattataa ttttatataa taaataagta aaaatagtaa aaaataaatt aagaaatgtt
     2521 tttaataaaa ataagcttaa gggggtcaac catgagtgca aaggtaacga atcaatatgg
     2581 aaatatagaa atagataatc aagtaatagc tcaagtcgta tatagagctg caatggaaag
     2641 ttatggctta gtaggtttag catataagtc taaaggaata gtagagctat taaaaggcga
     2701 gaacgcaact aaaggtgtaa gagttgagga agtagaagaa gatgccatag caatcgaact
     2761 ttatgttata atacaatatg gtacaaatat atctactgtt gccaataata taatagatag
     2821 agtaaaatat gtagttgaaa aaatgactgg cgtaagagtt actagaattg atattaatgt
     2881 acaaggaata agagttaagt aatttcatag gaggaaacaa atgattcaat atatagatgg
     2941 taaaaggtta agggagatgt ttatttcagg tgcaaacaat ctccaaaaca ataaagagtt
     3001 agttgacaag ttaaatgtat tccctgtacc agatggagat acaggaacta atatgtcatt
     3061 aacaatatct tatgccttaa aagaattagc aaaagttgaa aatgataata tatcagatat
     3121 cggaaaagca ttatctaaag gttctttaat gggagctaga ggaaactcag gagttatact
     3181 ttcacaaata atcagaggta tagctaaatc tatagaagga aaaagtaaat tatcaacaga
     3241 agatttagca aaagctttta aaaatggttc agacacagca tataaagctg taataaaacc
     3301 aattgaagga actatactta ctgtagttag agaaagtgga gagtttgcaa taaaaactgc
     3361 gaaaaaagag aaagatgtag taaagttttt aagtatgctt gtgaaggaat caaattcttc
     3421 attagaaaga actccagacc ttctaaagaa tctaaaagaa gctggtgtgg ttgactctgg
     3481 tggaaagggt cttgtactta tatatgaagg aatgttagct tctataaaag gcgataacat
     3541 agaaataaaa aatgttgacc tagatactaa tatttcaaca agtatggatt ttgcaaagag
     3601 tactactagc acagatgata taaaatattg ctattgtaca gaatttattt tagaaagttc
     3661 taaggtagaa gatactaaaa taagagacat aatgatggct tatggtgata gcctagcagt
     3721 agttggagat gatggagtca taaaggtaca tgtacacact aatgacccag gaaatgtact
     3781 tcaagaagct ctaaagtacg gtcaattatt aactataaaa atagaaaata tgaagttaca
     3841 acatgaaaat acattactgg atgtagaaga aaagaaagaa aatgatagtg aacctttaga
     3901 agaagaaaaa gagtttgggt ttatagctac ttctatgggt gaaggtttgg caaatatatt
     3961 taaagatttc ggagttgacc atataataga aggtggtcaa actatgaatc caagtactga
     4021 agattttatg aatgctataa agcatataaa tgctaaaaat atatttattt tcccaaataa
     4081 cagcaatata ataatggctg ctaatcaggc aaaagaattg agtgataaaa atataatagt
     4141 tatacctaca aaaaatacgc ctcaaggatt tgctgcatta gtaactttta atggagaatt
     4201 aagcgaagat gaaaataaag aagctatgat gaatgcttta aactcagtta aatcaggtca
     4261 agtaactttt gctgttaggg atacagtgat gaacgaaatt gatgttaaag aaggtaatat
     4321 aattggaatt gcagaaggga atttattatc agcaggagat tatgtggatg aagtaacatc
     4381 aaaccttatt gaaaagttgg ttgatgaaga tactgctata ataacattat tctttggtga
     4441 agatgttaca gaatctcaag caaatgaact acgtacttct ttagaagaaa agtttgaaga
     4501 tgtagatgta gagttatatt atggaggaca acctctttat tattacttaa tttcagttga
     4561 ataatgatta taagaacctg tatgaccaag tagcttttat aaaattggtt gtacaggatt
     4621 tttgtgtagt aggtgatata tttgttggat ttatataaag atgttcaata tgttaaggga
     4681 ataggaccta aaaaagcaga taaattaaat aaactgggta tatttacact aaaagaccta
     4741 ttgtattatt ttccaagaca atttgaagat agaaataatc taaagaaaat agcacaatta
     4801 gaagatggtg agaaagttac gataaaagca gtaatatcaa gtataaatac atttagccca
     4861 aaagaaggta tgactttaac aaagattgat gtaaaagatg agacaggctc ggctaaatta
     4921 gtgtttttta ataagagtta tataaaaaat acttttcgtc caggagattc aatattagtt
     4981 tttgggaagg ttaagaaaaa atttaataac ttagaactta catcatgtga attagaatat
     5041 cttactaatt cacctaagaa tacatgtaga tttatgcctg tgtaccagct tacatacggt
     5101 gttacaaata aagaaattat gagtataatt agaacagtac ttgaagataa agaattaatt
     5161 atacaagaat atatgccaca aagaattata gaaaaatata ggctatgtag tatagatttt
     5221 gcagttagaa acatacattc accaagtagt aaggaatcat taaaaatagc tttatataga
     5281 attgtatttg aagaattact tatactacaa ctagggttgt ttgtatttaa aagtggcaga
     5341 aataaagaag atggaataaa gtttgaaaca agtaaagact taaaaaaaat aataagtgct
     5401 ttaccattta aactgactaa agctcaaaac agggcattag atgaaattat acaagatatg
     5461 aatttagaga aaataatgaa tagattggta caaggtgatg tagggagtgg taaaacagta
     5521 gtagctctct tagcacttgc aaattgtgtt ttaaatggtt atcagggcgc tttgatggca
     5581 cctacagaga tattagcagg gcaacattat atttcactta ctgagtctct aaaagatttt
     5641 ggaataaatg taggtctatt aataggtagt cttacaaaaa aacaaaagga tacagtattg
     5701 gaacaaataa agaataatga gattgatata ttaataggta cacatgcact aattgaagat
     5761 aaggttgaat ttaataatat tggtttagtt ataacagatg aacaacatag atttggtgtt
     5821 atgcaaagga gcaaactttc attgaaagga gcaaatcctg atatattagt aatgacagca
     5881 acaccaatac caagaacttt agcacttata ctctatggag atttagatat ttctattata
     5941 gatgaacttc ctccaggaag acagccaata gaaacaattg ctattgaaaa aagtaagcga
     6001 gatagagcat ataataacct tgtaagaaga gaggttgaat caggaagaca agtgtacatt
     6061 gtatgtccct tggtagaaga aagtgaagcc atagaagcca aatcagcagt tgaacttgtt
     6121 gaggagttaa gagcggaata ttttcacgac ttaaggcttg ggttgcttca tggtaaaatg
     6181 aagtcaagtg aaaaagatga agtaatgaga ctttttaaag ataaagaaat agatatttta
     6241 gtttcaacaa cagtaataga agttggtgtc aatgtgccaa atgcaacatt aatgataata
     6301 gaaaatgcag aaagatttgg acttgcacaa ttacatcagc ttagagggcg agttggaaga
     6361 ggtagtcata aatcgtattg tgtactaatt tatgattcta aaacagatgt atgtagacaa
     6421 agaatggcta taatggaaga gaccaatgat ggttttaaga tttcagagaa ggatttagaa
     6481 ataagaggac ctggggaatt ttttgggaca aggcagcatg gtcttccaga acttaaggta
     6541 gcaaacttat ttaagcatat aaaaatactg aagttagcac agcaagaagc tcgatatata
     6601 ttgggtgaag ataataatct acaattaaaa gaaaacatgg cactcaaaaa agaaattata
     6661 gataaattta aagatacatt gaaagaaatt tcattaaatt aattttaaat tgtgttaaaa
     6721 tattactaat gtaataatgt gctgaaggga gattgtcata ttgagagtaa tttcaggaaa
     6781 agcgagagga ttaaaactaa atactccaaa aaatgaagat gtaaggccaa ctacagatag
     6841 agtaaaagag tctctattta atatgataaa ttcatatata atggaaagtg aagttctgga
     6901 tttatttgct gggactggtt ctttaggaat agagtgctta tctaggggtg ccaagtcatg
     6961 tacttttgta gatatcagta aggaaagcat agatattgtt aagtccaata taaaaaaggc
     7021 aagagttgaa agtgaaagta ttattttaaa ccttgatttt aagactgcaa tagataaact
     7081 taaattacaa aatagtaaat ttgacataat ttttatggac cctccgtatt ataaaaatat
     7141 gtttatagaa gctattgaaa aaattgataa ttctaatcta ttgaacgaag atggaataat
     7201 agttgtagaa catgatacga atgatttatt tccagataag atatgtaaat tggagaaaac
     7261 tagagataag aaatatggaa atactacatt aactttttat aagatggagg cataatatgg
     7321 aaaataaacc tagaaaagct atatttgcag gaagttttga cccaattact aatggacatt
     7381 tagatattat ttgcagggct tcgaagttat ttgacgaatt acaaatagga gtattaaata
     7441 atcctaataa aaagggtcta tttagctttg atgaaagagt aaaacttata gaaaaaagta
     7501 caagccattt aaataatata aaagttgtga cttttgatgg tttattaata aattactgtc
     7561 aggaaaatgg gattggagca ttagttagag gtgtgagaag tggtgctgat gttgattatg
     7621 aacttcagat ggcgcatatg aatagagagc ttaatccaga tatagagact ataattttac
     7681 caagttgtac aaaatattca tttataagtt catctttaat aaaagaggtt ttgctctttg
     7741 atgctgatat aaaaaatttg gttccaaaga ttgttttgga agaattaaag aaaaaaacta
     7801 gtgggggtaa ttgatatatg aagatagatt tagaaatgat tgaactattt gagcagttag
     7861 aggagatatt tagaagtgct tctacaatcc ctttctcaca taaatgcaca gtggataaag
     7921 atgaggtttt agcaataatt aatgatataa gaactttaat accagaagaa gtaacacagg
     7981 cagtatggat taataaagaa agaaataaaa taataagtca agctaagcaa gatgctacaa
     8041 atatagttga acaagcgaaa aaagaagctg agagaataca acaagaatat caagataata
     8101 tagaggaatt aaagaaaaat tctgaagatg tagtaaaggc atatgtagaa tccagtgaac
     8161 ctgttgttca agcagaccaa agagcaaaag atatagttga tagagctgag agaatagcca
     8221 atgagataag aattgggtca attgaatatg cagaggatgt gctttctagt gttgagtata
     8281 atttaagaga aatattagaa gaagtaaaaa gagataaagc tgaacttaga cctaaaaaat
     8341 aaattttaaa taaataaaaa tggagctatc acacaaaaaa ttccaattta tgtgtgatag
     8401 ctctctttta atatagcaat tttttgttat ataattgtgc tttttagatt acttatatct
     8461 tattttgata ttataatgta gtttaatctt ttatttgttg aaatcaactt atttatttgt
     8521 ataaataata taggttattt tcaaattttt ttaataattt taccactgtc ttcttttaga
     8581 ttagagttag actgtgcaaa attataaaat aatactaaat catgtgcaat tatttggtat
     8641 ggcactacat gtagaaaata tagataataa aattaaggag gtagtatgaa ggaaagggct
     8701 aaaaaaatat tagattttat tattaaaaat aacaatgtaa catcacaaga actacaagaa
     8761 aagtttaatg tttcaaagag aactatttat tacgatattc tagctataaa tgaacaacta
     8821 ggaaaaagtg gtaatataaa aaatgtaaaa cacaaattta tatttgaagg taatttatgt
     8881 gatgcaagga aaataatttc aactgaagaa gataaatttt tagatagtga ttacagaaaa
     8941 acttttatat taaacaagat attacttggc gaaaaaatat caatagaaaa actaacaaat
     9001 gaaatgttac tttcaaaaaa cacagtagta caaacaataa ctgatgtaaa aaaatacctt
     9061 caaacaatgg ggttaagatt agagtataag ggaaagtaca aaattatagg tgatgaatac
     9121 gttattagag agctgtttct gataattgtt caagaaaatg tactggaaat taattctata
     9181 agtgaggaag taagtagttt cgatacaaaa ggacatataa aacttactga ttattcttta
     9241 ttaaatctaa ctaagtttgt ggaatttcta aataaaagga taagagatgg gaaaacatta
     9301 tactcatata aatatctaaa tgaagctaaa aaaattagtt actttagtaa ttgtaaagaa
     9361 cttttatgtg aagaagctaa tgaaaatgaa caagcctata tatgcactta cataagttca
     9421 cttcccagct taaatagtga agttaaggaa gatgttgttg aagaatatgt agataaactt
     9481 atagataagt ttgaagtaaa tacagcaata aaattagaaa gtaagcatga gtttaagaaa
     9541 aatattttaa ggcacctcca ctcttcttac aacagaatac gatttaagtt tcctattaga
     9601 aatccaatgt tagatgagac taagtataaa catgagtcac tttataaaat tataaaatca
     9661 attatagaga atgaagaaga gtttcctgta tttgagggga taagagaaga agaaatagga
     9721 tttatagcag cgtattttgg aggatattta agagggtcta gggataatgg tctaagaaga
     9781 aataaagtct tactagtttg tcctaatggt cttatggtat caaagagttt ggaaatacaa
     9841 ctatataaat acataccaac catagaaata gtaggtatag tatccattaa gcaactaaaa
     9901 gaagtaaatg tatactacga ttacataatt acaactatag atatacaaaa tgtaaacaac
     9961 gtaattgttg taaatcctct tttaacatca tcagatgttc aattacttat gaacaaacta
    10021 ataagtgtaa aagaaaacga gaagtatttc aatcttgaat taataataca agctataaga
    10081 aaaaatggag ttatcaataa tgaagaagca ctaaaagctg atttattaaa cataatacac
    10141 aagatagatg aaggagagat gtatcaacca atgttaaagg aattaattaa tgcagaaagg
    10201 gtcaatataa taaaaaatgt tagagattgg aaagaagcaa taaaaatagc ctcaaagcca
    10261 ttgttagaag ataactctat agaagagtta tatatagaaa atatgataaa gtcagttgag
    10321 aaatatggac catatattgt tttagctgat agatttgctc taccacatgc aagttctaaa
    10381 gaaggagtaa acaagcttgc tatgtcttta ctaatagtag aagatgaagt tgatttatta
    10441 ggaaaaccag taaatatatt tatggtatta gctgctgtag ataacactac tcacattaga
    10501 gcattagcat cactatcaga gatgatgtac gaggaagaaa atgtaaaact aataattaat
    10561 ggtgataaaa gttcaataat agaacttata aataagcaaa attaggggga atgaaaaatg
    10621 aagatattag cagtatgtgg ttttggtgta ggttcatcaa tggtattaaa aatgacaatc
    10681 caaaaggctc taaagcagtt aggtatagaa tgtgaagtag aaaatacaga tataaattca
    10741 gctagaggtg ctgattgtga ctgtatattc acaagtcaag aattgttaaa tgaattaaaa
    10801 ggaacttgta atgtagatgt atatccagtt aaaaagtaca tggatttagc agaagtcaca
    10861 gaagttgttc aaactttttt aagtgataaa aatgataaga aatagtataa gttttaaaaa
    10921 gtaaatttaa gttaatcaga ataaaataaa aacttactaa gttcaaaaat agtaagaaag
    10981 agaggaggga tttagtggta gaaaacttaa acagttgacc tatcactaaa aaatataatg
    11041 cttgattaaa aaatagctac gcacactcgt gaccttagtc atgagttagt agctaaaata
    11101 gtcagcatat agggaaacct atatgtagag ataggataga acctatccaa tactacttga
    11161 attgctggaa acccctaaag ctaaccaaac taaaacataa ggatgaaata aacctaagtg
    11221 tgaaagttac gaaagtagaa aaaattggtt agatggtata aggttaaacc ctaagtactt
    11281 taaaatgggc aatcagcagg taagctccga ataggagaaa cttcaacgac tattcctctt
    11341 gagggaagta cactacaagc tattggtagt ggaagtgggt agaccttaac ggataatgcc
    11401 gaaggataag atatagtctg tgcttacgtg aaagtgtaag aagttcataa gagaactgca
    11461 taggtagtag cgaacttatg tgaacgacaa cctcaaaaat gataaaagtt aagaacttac
    11521 ggttcttata tatgttattt aaaaataaat ataaaaaacc ttcaaaatac ttgttttaag
    11581 taattgcata ttttaatatt atgataagtg atgaaaaatg gaagttactc atggtagagg
    11641 atatgcatat agtatgtcaa atatagacat ccattattat ggggagacat ctttggaatc
    11701 ctagttattt cgttgctact gtattagaaa atacagagga gcagatagga aaatatatta
    11761 ataatcagaa aaaacaagta aagtgaggtg atagtagtgg aaaaagctta taagtttaga
    11821 atgtatccaa ataaaaaaca gcaagagtta attaacaaaa cttttggatg ttgtagattt
    11881 gtatataata agtatcttgc taaaagaata gaagtttata aaaacgataa agaaactttt
    11941 acttataaac agtgtagttc tgatttgacc aatttaaaaa aagaattaaa gtggcttaaa
    12001 gaacctgaca aattttcact acaaaacgtc ttaaaagatt tagataatgc atataaaaaa
    12061 ttctttaaag aaaaagcagg atttcctaaa tttaaatcaa agaaaattaa tagattttca
    12121 tataaaacta actttacaaa tggaaacatt atgtattgtg gtcaacatat aaaactacct
    12181 aaacttggta tggttaagat aagggataaa caagtacctc aaggtagaat acttaatgct
    12241 gctatatcta aagaaccaag tggtagatat tatgtatcat tatgctgtac tgatgtagat
    12301 attgaagcat ttgaaaatac taataatcag ataggtttag atttaggaat taaagaattt
    12361 tgtatctcaa gttgtggaga atttatagaa aatcctaagt atcttaagaa gtcattaaac
    12421 aagcttgcta aattacaaag agaattatcg agaaaaacaa tcggtagttt aaatagaaat
    12481 aaggcaagat taaaagttgc aagacttcaa gaacatatag ctaatcaaag aaatgatttt
    12541 ctacaaaaac tatctactaa attaataaaa gagaatgata ttatttgcat agaagattta
    12601 caagtaaaga atatgattag aaatagtaaa ctatctcgct taatttcaga tgtatcatgg
    12661 tcagaattta ttcgtcaatt agaatacaag gctaattggt atggtagaca aattgtaaag
    12721 gtaggtaaat tctttgctag ttcacaaata tgtaataaat gtgggtataa aaacgaggaa
    12781 gttaaggact taaatataag agaatggatt tgtcctagtt gtaatgaaac tcacgataga
    12841 gatataaatg caagtataaa tatattaaaa gaaggattaa gactaataac gattcaaaat
    12901 aaataacata tgtaagaacc gtaggaacta cggggatagc ctgtggagaa ttagtaagac
    12961 atattttagt atgcaaaatt ctatgaagca ggaaccctgt gactttagtc atgggaggtt
    13021 cagtttattt caaataatat tttacgtaat ccatctatgt taattggatt aatagcagca
    13081 tttggtcttg tactacagaa gaagaaagca tctgatgtta taaaggggtc actacttgca
    13141 gctttcggaa ttataattat ggagactgga acaggtatgt tagttagttc aatagcacca
    13201 attaatggag ctttccaaag tataagtggt ggtgcagtaa caaaaggttt aagtgatgtt
    13261 acttttactg caagttatgg tggaattata ggtttagcaa tgtttgcggg tttagctatt
    13321 cacctaatga tagcaagatt tactcctatt aagacaatct tcttaacagg tcacatgctt
    13381 tattggttcc cattcatatt tgtagcttca ggagttgaag ctggattaaa tggtggagta
    13441 ttaatagctt ttgcagcagt attatcagca ctctattggt caataatgcc ttggttatta
    13501 agaaaatatg tgtttgatgt tataggagat gattctttta cattaggtca tccatctggt
    13561 tgtttagcac ttatatcagg atttgttgct aaattagtag gaaataaaga aaaatctaca
    13621 gaagatataa acatacctca aagtttatca ttttttagag aaatatctat aagtggtgga
    13681 atggcagtgt ttttaatgtt tatagttgtt gggatagtaa taccaggtgc gtttaaagat
    13741 agtaatgagt ttattggaac gtatgcaata aagcaaggtc tttcttttgg agcaggtctt
    13801 ataataatgc ttcatggggt aagaatgcta ataaatcaaa taatgccagc atttcaaggt
    13861 atatctgaaa aagttgtgcc aaatgcacta cctgcttttg actgtccaat attatttaat
    13921 tataaaccaa atgctgtatt aataggattc ttagttgcta tgctaacatc tacagctatg
    13981 attttaatat ataactactt taatgtattt aatttgctac ttataccact tgttataact
    14041 tcattctttg agtgtggagc agcagcagta ataggagaat cacaaggtgg tatacgtgga
    14101 gctataatag gtacagctat agctgcaata tttatggtag ttttagtagg aatatctgct
    14161 gtaatttact caggaactat tcaaaattgg atactaatat ttggaggtaa tgacctttca
    14221 ttatttggta taatcagtag gtttataggt tcactatttg gaggaatatt atagatatga
    14281 aatttaaata catagatgag ataagaagat taattgatat aatagaaaac gaagaaaaag
    14341 tcaatatgga aaaagcagta caaatctttg ttgaagcaat agaaagtaaa aatgctatat
    14401 tttcatttgg agcttcacat gcaggaatat taacagaaga attattctat agagcaggtg
    14461 gctgtgttct tataaatcca gtatttgcaa aatctctaat gttagataca gcgcctataa
    14521 ctcacacaag taagatggaa agattagaag gatatggtac agctctagct gataaaactc
    14581 caattaaaaa aggagatgta gttatagttc attcagtatc tggtagaaat ccagtaagta
    14641 tagagtttgc tatgaaagct aaggaaaata aggcaatagt aatatgtata actaatttaa
    14701 gttactcaaa aagtgtatct tcaagacatt cttcaggaaa aaaactttat gaagtatgcg
    14761 atttagtgat agataatcat ggtcaaaagg gagatgcctg tgtagagata gatggattaa
    14821 accaaaaagt tagtccaact tctactgtaa tagcaactac tgtgatgaac tctattatag
    14881 ctcaagctac tcaggaatta gttaataaag ggctaaagaa tcctccgatt ttctatagtg
    14941 ccaatattga tggtggagat gagttaaata agaagatttt tgatgaatat aaaagtgtaa
    15001 ttcattatga atattaaggt taaaaaaata tgttaatgaa ctttgaagtt tatcaacaaa
    15061 gaatattaat aatatataat aaatgctatc atttaaaagc tgtaggtaaa aatctacagc
    15121 tttttattat tgtagattaa aaagatgcgt aattaaattt taaattggag aaaataaatt
    15181 ggaaggtatc aattataaat attagaaacg ttgtcataga aaaaaactat attaaaaaaa
    15241 agtacataga aaaaactgat tagacaataa atacatgctt tgataatatt ttaaatggaa
    15301 tgaaaatgtg tagaattata atgaaaaaag aaaataaaaa tgaaaaaaga aaataaaatg
    15361 gagggaataa aagtgggtat gttaaaaaaa ttatgttcac tagctatggt agcaattatg
    15421 actacaacac ttataacagg gtgttcaagt ggaaatggaa aagattcaaa aaaggatggc
    15481 gaaaaagaaa aactagttct ttggatgcca ccagtagaaa ataatatgaa ggaagtttgg
    15541 gaccctattt tagataaatt tgaggaaaaa aataactgtg aagtagattt acagataatt
    15601 ccttgggata attattctga aaaatttgca acagctataa gtgctggcga aggtccagat
    15661 gtaggatata tgtatgcaga aatgtatcca cagtttatag aaagtggagc agtagaagat
    15721 ttgactaact atgcaactaa agaggataaa gagcaatcta tttatataaa aacatctgaa
    15781 atgatgggtg gaatgtatgg tatgccattc caagcagcaa atccaggggt attatactat
    15841 aacaaggata ttttagagaa actaggagaa aaacctccta aaacatggga agattttaaa
    15901 agaatttgta aaaaagcaac gcaagatact gatggtgatg gaaagataga ccaatgggga
    15961 ttagctcagg gatggggagc taaaactttt ggtaacttaa actggaactg gtatccttat
    16021 ttatggcaag ctggtggaga tatatttaat gatgatttaa aatcggttaa atttaatgat
    16081 aagagtggtt tagaagcagc taatttctta aaagaattac aagcatatgt gcctgaagat
    16141 tctttatcta aagatagtaa tgaaatgata gaaagcgtat ttggaccagg gaaagcagca
    16201 tttacaataa tgttatcttc tgctgcaaca agtgtatttg atgaatcatt cccggattta
    16261 aattggggat ttgtaactgg actagaagat aagaaagcag caacttttgg agcagtagac
    16321 catttatctt taatgtcatc tgctaaaaat aaagaacttg catacaagtt aatacaacat
    16381 atgttaagtg ttgaatcaat gacagaattt cataaagcaa taccaagagc accaataaca
    16441 aaaggtgaac catatcaagg tgatgaaaga ttcaaagaaa tggtggaaaa tgacaaaaat
    16501 gtatatagac cattagtagt aggacctcat ggagttgaaa tatatgaata tttatggaaa
    16561 gaacttcaaa ctatgatttc aggagataaa actccaaaac aagcgttaga tgatgctgca
    16621 aaatattcaa atgatttatt agcacaataa aagtttaaag ctgtaatatg ttgtaaagga
    16681 ctttacagat taaaattagc attgtattta taaatacaat gctaatattt atattggaga
    16741 ggataaaatg gaaaacttag ttagagagtt tgaagaaaat tcaaaaagaa aaaaaacaaa
    16801 attaaatatt aaaccatata tgtatatatt gccattggga ataattttag tggcgtttta
    16861 tgtgatacca ataataatgt caatatactt tagttttaca aagtataata ttattagtcc
    16921 agctactttt ataggtttag aaaattataa aaaattattt acagatgaaa cattaaaagt
    16981 atctattata aatactatta aatttactgt tgttgtagta ccttgccaaa ctatattatc
    17041 attgattttg gctgtttgga ttacaggaaa aggaaatagt aaaatagctt catttgctaa
    17101 aggtgctata tttataccag tattatcatc tatggttttg ataggtatgg tatggagggc
    17161 tttattgaat ggagaaggtt ctattatata tcaggtatta ggtgcctttg gaatagaatc
    17221 atctaaactt ttaggagata gtaagacagc attacctaca cttatgttta tatctatgtg
    17281 gaaaaatata gggtatttta tggtaattta tatttcagct ataatgaatt taccaaaaca
    17341 ctgctatgaa gtagcgaagg ttgatggggc tacaaaactt caagaattta taaaaataac
    17401 tgtaccttta ttgaaaccaa ctacaataat ggttgtattt ttagggtcga tatggtcatt
    17461 acaagtattt gacttaattt atactgtgac aggtggaggt ccaggtatat ctactatgag
    17521 tatagttatg catgcattta acttaaactt taaaaacttt aattctggat atgcaatgac
    17581 tgtagcaaac gtattatttt tgttaatagc agtagtttcg attttacaaa ataagcttgt
    17641 aagacgagac aattcagatt tttagaaagg taggtataat atgaaaacag caagaagatt
    17701 attaagtgat ggtttgcttt tatttatagc gtgtgcaagt ttggtacctt ttatatacat
    17761 gttgataata tcattgaaga taacatataa ctcatatagt ttggatatat ctttttcaac
    17821 agtaaccctt caaaactata tagatatttt tactaaaaaa ggatttgcac aatatttttt
    17881 taatacagca atagtatctt tttcaggtgt attattaaat ttagtattta gtacattagc
    17941 aggatattct tttgcgaaga tggattttaa aggtagtgat aagttatttt tatttatgat
    18001 aatgacttta attatacctt cccaagttac aatgatacca ctatatataa taatgaaaca
    18061 cttaggatgg ataaatagtt atcttgcact tattatgcca ataccaactg catttggcgt
    18121 atttataatg agacaagcta ttttaggagt accaaaagag ttattagaat cagcaaagat
    18181 agatggttgt tcggatttta gaattttaat acaaatagtg ttgcctttaa ttaaaccagc
    18241 attaattaca ctagcaatat ttacttttat gggagcatgg aacgagttta tgtggcctct
    18301 tatagctaca actaaagata caatgagaac attaacagtt ggattatcta ctcttaagac
    18361 attccagata actaattatg gacagatgat ggcaggggct acaataacat ttttacctcc
    18421 atttattttc tatttgattt tacagagtaa atttgttgaa ggtgtgtctt tgtctggtat
    18481 aaaaggttaa atagtctaat ttaataatat ataaagaaac ttatataaat atggaggaat
    18541 ttaaactatg gaaatttcat atgtactgaa agattgcaca ttcaaacaca ataaatactc
    18601 agttgtagaa gctgaaactt ggaaagataa ggatttggat attgtagtag ctaaaggtga
    18661 aaaatttgca tttcaaatta tgttaaaagc aacagaagaa tttaattgta caattgataa
    18721 aagtagtgct atatcatgga aaggcctggg aaatagagta aggttagcat taaatatgcc
    18781 taatagttta gaaaataatt ttagtataaa tatattagga tatgttcaag atgatacaaa
    18841 ggcatttgtg aatgatgcta ttttaaggga taaagatgtg ttagtagagc aatatttgcc
    18901 tcaaacattt tggattgaag gtcaagtacc agaagatttt gaagaaaata accttgatat
    18961 aggtattgat atatttaaaa gctttggata tgaagacgaa gaaaaagttt gtacaattga
    19021 tgttcctgta aaagtaagaa atgttgtgct aaagccttta gatgaaagca agttcttttt
    19081 agatttatgg caacatccaa gctgtttagc tagaatgtat aaagttgagt tatggtctga
    19141 tatacatttt gaaatagtcg ataactattt aaaagaatta gcatctttag gagaaaaagt
    19201 tgcaacagtt atagtttcag attatccatg ggcagggcaa agttgttata aagtttataa
    19261 aaatccatca aacttatatg aatataacat ggtaagtgta tcaaaaggat tggatggaaa
    19321 gattaaatgt aactttgaga gtatggatag gtatataagc atagctgata agtataaaat
    19381 ggctaaggag atagatttat ttggtctatt aggtaattgg tgtgctggtg agtttggaaa
    19441 tcctgtagaa ggttataagg accctataag agtaagatat tttgatgaaa aggataaagt
    19501 atttaaattt ataaacaata caaatgattt aaaagaatat ataggattag tattaaatca
    19561 tttaatagag tgtggattat gggacagagt aaggattata gctgatgaac caaataatcc
    19621 agaagtagtc aaagagtgta tagaatttat aaattctact gtaagcactc atcaagttaa
    19681 atataaatca gcaactcacg accaaaactt tttagacaga gctaaagatg aaattgatga
    19741 tatgtcaata aatttaaaac ttactataca aaattataaa gatatagaaa gtcttaagaa
    19801 aaaaatcaat gacaagggtg ggatattgac ttggtttgta tgctgttttc cagagaaacc
    19861 aaatagtttt ttaagttctc cttttgtaga aaatagaata ataggatggt atacttatta
    19921 ctttggtctt gatggatttt taagatggga ctataattta tggacagaag acccttggaa
    19981 agattcaagt tacaaattcc ctatttggaa agctggagat atgttctttg tttatcctgg
    20041 aaaagattta aagccagtta gaagtgtcag aatggagaat ttaagatttg gaatacagga
    20101 ctttgagtta tttacaatgt tagaaaaaga aaagggaaga gagtatatag aagttgagct
    20161 tatgcaagaa ttattaaata aaaaagaaaa tgctgaaatt aaaggttttg gtgacataga
    20221 actaggatat tcacttgata attggggata tgataaagtt aaaaaatatg tattagagtt
    20281 gttagacaga gtataaaaaa tataagagtg tctcaaaatg aactttttag ttcatgaggc
    20341 actctttttg tatgaaatca atatagtttc attgtttcaa caatgaaaaa taggattatt
    20401 tctattctaa gacagccctt tagtttttta gttgtattca atgttttaaa actaactata
    20461 aacgctataa agtttataaa tgacaataat tatatataac atgatgtact ttaatttgct
    20521 ataataggga ttgaataata ttatatggaa gataacattg tttgggggga ttaatataaa
    20581 aagaataggt attattataa tgtttttttt aagtatatct tttattggat tttatcttgt
    20641 aataagagat aacagtgata cagttaagat gcaacgaggg ataaatatag gtaatgcact
    20701 agaatcacct aaggattttc cgtgggatgt aaaaatgtca aataagtttt ttgatgatat
    20761 aaaagatgca ggttttgaca cagtacgtat accagtaagg ttttctgatt atacgtcaga
    20821 ttctgataat tttaaaattg atgaagaatt ttttaaaaag attgataaat atgtagatta
    20881 tgcattggat aaagacttaa tagttgtgtt ggatttacac cattttgaag aaattatgaa
    20941 agaaccaaga gttcataaag aaaagttttt aaaaatatgg caacagatag ctaataggta
    21001 tcaaaaatat gataaaaaat tggtatttga attattaaat gaacctaaag aaaatttata
    21061 ttcacagtta ctaaacgaat acattgaaga agcaataaag ataattagaa aaactaaccc
    21121 taaaagaact attatagtag gaccatataa tttctatcaa attgattatt tgaatgaatt
    21181 aaatattcct aaagattcta atatagtagt ttcctttcac tattacgagc caaatgattt
    21241 tgcatttcaa ggaaatatat atcataaggg atttgagcac ttaagcaata ttacttggga
    21301 aggaacaaac gaacaaatgg attatttaaa aaaaaggttt gatactgtgg aaaattgggc
    21361 aaataaaaat aaggtaaaaa tcttcttggg tgaatttgga ataacaaaag aggcaccaga
    21421 agcttcaaga agagcatgga taaaagctgt aagagaagaa gctgaaaaac gaaacttttc
    21481 atgggcttat tgggagcttg catctggatt tggtatatac aatcaaattg aagggacttg
    21541 ggatagagat atattaagtg cattaataga aaagaggtga ttttatgaag aaaaattcaa
    21601 aaaagatact tataacttta actattatta ctaatgtcat atatatattg tggagaattt
    21661 tttacacagt acctaaagaa gaagggaagt ttgccttaat atgtgccata atacttcttt
    21721 ttgtagagat aatgggcatg atggaaatgt ttgtgcatta ttatggtatg tcaaatattg
    21781 agtaccctga aaaaccaatc ataagtgaag aactttatcc acatgtagat gtattcattg
    21841 caacatataa tgaatctgtt gatttggtga gaaaaactgt aaatgggtgt attcatatgc
    21901 agtatccaga taagaaaaag gtacatatat acatatgtga tgatggaaat cgtgaagaaa
    21961 tgtgtattct agcagaaaaa atgggtgtaa attacataac tagaactgaa agagaaggag
    22021 caaaagctgg aaatttaaat aatgcaatgc aacatacaaa ttcccctttg attgctacat
    22081 ttgatgcaga tatgatacca atgcatgact ttttaattgc tacagtacca tactttttga
    22141 aaaatgagca agctaaaaaa gatggagaaa aagaggagta tgaaaaggtt ggttttgtgc
    22201 aaactccaca gagtttttat aatccagact tgtttcaatt taatctgcac tctgaaggaa
    22261 ggattccaaa tgagcaagat tatttttaca gagacattca attggctaga aataggacaa
    22321 attctgtaat ttatggtggt agtaatacag ttatttcaag ggaagcactt gaagaagttg
    22381 atggatttta tacttattct attacagaag attttgcaac aggtatctta attcaaagta
    22441 aggggtatag atgttatgct attccagaag tacatgcatc aggtttatct cctacagatt
    22501 taaaaagtct tattaagcaa cgtgaaagat gggcacgtgg atgtatacaa actggtagac
    22561 gtctaaatat tttgtttagg agaggtttag gtttttggca gaaaataagt tatatttctt
    22621 caattactta ttggtatgca agtataaaac gctttgtata tataatggca cctattttat
    22681 tttctgtgtt taatgtgatt gtagtaaaat gtacgttact tcaagtattg gtattttggt
    22741 tgccaatgta tattttaagt agtttatctc ttaaaatatt ttctcaaaat ataagaaata
    22801 ctagatggac caatatatat gaaactataa tgtttcagtc tttaatgcca gcagtaatac
    22861 ttgaaacgtt tgcaatatct aaaaataaat tttcagtaac aaataagagt aaattagaag
    22921 aaaatagaat gtataagttt ttacagggaa taccttattt tatttacatg gtcttatcta
    22981 tcataggaat tttaaaaatg tttgtagcaa tatttaaaat gagttcaatg acatattcag
    23041 ttgtattatt ttggttgatt ggaaaccttt ttaaccttgt tatggctaca ttatttatat
    23101 cagggagaca gcagttaaga aagtctgagc gttatattgc tgaaattgat tttaagttaa
    23161 agcaaaattc ttatgttctt tcttcaaaaa caattgatat atcagaaaat gggtttgcat
    23221 ttttgctaga aaatccagaa tatatttccc cagaagaaga gtttgaggta gagtttagag
    23281 aaaaatctgg aaatgaaatg tatatagcta atatgaaagc aaagattgtt aatgttgtgg
    23341 aagtaaattc aaagtggaaa tatgcagctt atattacaca tattgaagat agtgaaatag
    23401 ataattggat gtgtattgtt cacgatagaa tacctacttt accaatgaca atatctaatc
    23461 aactaggttt ctttgatgat ttgcaaatta atgtaaaaaa acgtattgaa aaaactagaa
    23521 cattatcacg aagaagtcca agaatcaata tgaattttca gatggatata aaaaatacag
    23581 gtaaattaag aatagtgaat tttaattatc aatatgtatt attaaatttt gagaataaaa
    23641 gtatttatcc taaagaaata gcattggaaa ttaatgaaaa tattgtgtta gaatgtgatt
    23701 tgtgtgaggg taaaattgat gaaagaggta tcttatataa agtaaataac attgacagca
    23761 taatgcaaaa tttcttttta agagatgaaa tgatggattg gatattacaa aataagaaaa
    23821 ttcttgattc aaagcctagt gaaaagaagg aaaaaagtat agatgagttt gaacccatgg
    23881 aatatattta ggagagatga aatgaagact ttaaaagtgt ttttttgttc tacaaaatat
    23941 cttattttgg tgtttatttt ttttataaat actactttta taagttcagc aacaagtgat
    24001 aaaattttag atttatcctt taaaaagata gagactgact taagttctaa gattacatat
    24061 gaggatacag gtgtaaaaat agaaacggat agttctaaaa gcgataagga acgttattta
    24121 tatatttacc aaaatataaa agaaaattgg agcatgtata ataattttta tattgaaatt
    24181 caaaataaaa ataaaagttc tcagagaata aatttaagca tacaaagtaa aaatatgctt
    24241 gaatttagat taaaagaagg tagtgaagta tttttagaag gtaaaaatat aatatattca
    24301 gataaaataa aagaaggatg catagaagta ccaggtgaat ttgaaggcaa aatttatgtg
    24361 aactttaata gccttataaa tgaggaaagt aatgttgtgc tggactcaaa tatgctttca
    24421 aacattgtta gttggggaat tacgtttatt ccatctgatg aagaacataa tattgttatt
    24481 ataaaaaaga tatctttatt gtctgaagag aaattaagat ttttaaataa tattaaaata
    24541 attggcgatg aagaagtaca aataccagta ttgggtcaaa gtataagtca gtatgaggta
    24601 ttaggtctta aaagtgattc taaaattaag tactctctta tgggaaaaca agataatgtg
    24661 agtatatctc aaaacggtaa attaacatta agtaataaag ccaaacctgg acaaattatt
    24721 ttacaagtta atgttgatga taagtttaaa attggaaaga aaataacatt aactgaatct
    24781 tggagtatta ataaaaaaga taaggatggt gttccatata cattagtatc tcctgagcaa
    24841 tcaccaacag tacaagatat gaaaaaaata aattttatga ataatatcat aacgtttgtt
    24901 cgaattttat ttgtatctct agtaataata tgctttggaa tatatttata ttggaaaaaa
    24961 tgttcaaaaa ctaagtaaag agaggaggtt agattgtgtt attttcaagt ttaatttttt
    25021 tattttattt tttacctata actttggttt tatattatgt atttaggttt aaccgaacga
    25081 tacagaacat gatattgctt gctgttagtt tattttttta tgcttgggga gagccaaaat
    25141 ttgttgttat catgatagtt tccataataa tgaactatat atttggttta ctggtagata
    25201 gatataggga aagtaaaata aaagtaaaag tttttttagt tttgatgtgt gcatacaata
    25261 taggtgtttt atttatattt aaatatctag cttttgcgct aagaaatatt agtgaactta
    25321 ttagtactga attgactata cctaatatta ttttacctat aggaatttca ttttttacat
    25381 ttcaaggaat gtcttatgtt atagatgtat atcgtagaca tggagaagtc caaaagaatc
    25441 ctttttatgt gggactgtat atagcatttt tcccacaatt aatagcaggt cctatagtta
    25501 gatatgagag tgtggcagaa caaattctca atagaaaaga aacttggaat aaattttcta
    25561 ttggtacttg caggttcata acaggtctag gtaaaaaagt attaatttca aataacatgg
    25621 ctatagtagc agactatatt tacactatga atagtcaagg agaaatagct gcatcacttg
    25681 catggcttgg ttcaatagcc tatactctgc aaatattctt tgatttttct gcttattcag
    25741 atatggctat aggcttaggt cttatgtttg gttttaaatt tgaagagaac tttaattatc
    25801 catatatatc aaaatcaatt agtgagtttt ggagaagatg gcacatttct ttgggaatgt
    25861 ggtttaagag ctatatatac tttccgcttg gaggttctag agtagcaaat aaagatatta
    25921 tgattagaaa tatgtttata gtgtggttat tcactggaat atggcatgga gcagaatgga
    25981 catttgtaat ttgggggatt ttaaactttg ttttcctaat tatagagcga tttataatgt
    26041 ttgaaaagat tgaaaatcac aattttataa aacatattta tgctttatta gtagttaatt
    26101 ttggatgggt attgtttagg tcacctaatt taaaagaagc atataattat tttaaagcta
    26161 tgtttggagg caatggagct atatggagtg attatacata tatgttttta aaagaatact
    26221 gggttttctt tatatttgca tttattttta gtgtacctat tgcaaagaaa atcaataagt
    26281 ttgttgttga acaagctaaa tatagtatga tatttaacgt attttatcca atctcagcta
    26341 ttttattgtt ttttatttct gtgacatatt tagtaactgg aagctataat ccatttattt
    26401 attttaattt ttagttggga ggaaaagtat gaatagattt tttaaagtaa gattaataac
    26461 tgcaattatg ttcttaatta ttttattcgc tttctctatc tttaatgttt gttattctgg
    26521 aaaagatacg atacaggaaa taaaaaattc aatagctgaa aaaagtagtt taaaagatac
    26581 aatatcatct attgataata cagtgaatga caaagtaata tacaaaaata cgttcattga
    26641 aacttatggc tatttacaaa aattaatggg taaaaatgag ttttcaaaat ttagtgttgt
    26701 aaaagatact tctggaaaga tgcattatac atattttgct aaagctccaa atccagttgg
    26761 tgtgatagca gatagggtaa gaaaattttc tgatgaaatg gaaaagcaaa acactaaatt
    26821 ggtttaccta atgactccag ataagtatat aaaaggagtg acaaaatttc caaaaggaat
    26881 accttattct tatagtaatg agacagcaga taatttttta aatcaactaa aaaagtataa
    26941 tgtggactat attgatttta gagaaaatat tttaaagagt ggtattccaa aagatgattt
    27001 attttttaaa acagaccatc actggaaagt agaaacttct ttttgggcat ttggagaatt
    27061 agttgagcaa cttaataaaa aatacaatat gaatttagat gaaaatcatt attatagaga
    27121 taaggacaat tataattcaa ttgtatatcc taagtcattt cttggttcta tgggaagaaa
    27181 gactggtatc ttatatggtg gtatagatga ttttacatta atttatccta aatttaagac
    27241 taattatact tattatacag attctaaatc tcaaaaattt gaattaaaag gaaggtttga
    27301 agagagttta atattatcat atccttttaa tgcagacctt gatttaatgg atggtcaatc
    27361 agataagtat tttacatatt tattggggaa cagaccatta gttaaaataa aaaatataga
    27421 aaatccagat ggattaaaag tgctgtttgt taaagattct ctaattgtac caacagcagc
    27481 atttttttcc tctgtttgtt catctataga tatgatagac cctagatatt atgatggaga
    27541 tatactagaa tatgcaaaat cccatgacta tgactttgtt tttatatctg tatatcccca
    27601 aaatcttaca aaagaatttt ttccgttttg tgagtaaatt tctaaaaaag ttttctgata
    27661 aaataaatgg ctaactggta aaaagttagc catttatttt gagtgttatt ctaagaaata
    27721 ttttttttac taaatacttc tttatctatt tttccaagtg attttcctac agtaattgat
    27781 gataccatag caccattaac atttatcata gtacgtccca tatctagtat agggtctatt
    27841 gcaagtatac caccaactaa tggaaaataa gaaccaagac caactccaga gataacaact
    27901 gaaacggcca ttgtagcagt tcctggaaga ccagctattc caaacgaact aattgttata
    27961 acaattaaca acataacata aaagctcata tccatgttta ctccagccat attggcaagt
    28021 gttacagcca ttaaagcagg atatatacct gcacaaccat tcatacccat atttgcacct
    28081 aaacttgctg taaagcttga tacaccctca tcaactccgt gattttgtac tagactttct
    28141 atcgttacag gcaatgttcc taaactagaa cgtgaagtaa atgccagaag taaagcttca
    28201 gttgaatttt ttatataagt aatgggattt agaccattta aagttataaa aattaaatgt
    28261 ataataaata atattgcaac tgatatataa agagctaata taaactgtaa aacagatact
    28321 attgaactaa taccttgtga agttattgta tttgcaagca gagcaaccac agcataaggc
    28381 ataaatttaa taacagtcat agacacactt acaattattt tgtaagatgc ttctataaaa
    28441 tctataaatg gttttattgt ctcaaaatgt tttttactta atctctttat tgaggtagct
    28501 ataaatgtag caaatattat tgtagctaca atatttccat ttgccatagc cattacagga
    28561 ttagatggta gtaatcctct taaagtatcc acaagagagt ttatttcttt aagttcttga
    28621 ttttcaacta aagtaggcat attagctcct acacctaact tcattatatt tgcaacgata
    28681 attccaacaa ttgcagctat agcagtagta gcaagaagca tacctaaaga tttaaatgtc
    28741 agagttccaa gattgttatc tttcatattt ataattactt ttataatgga taagaaaact
    28801 aaaggtacta ctaacatttt taataagtcc ataaatccag aaccaaatag gctataccac
    28861 ttacttactt cttgtagcca agtgacttga gttgtatcat taggaaaacc agcaaaagct
    28921 tgtattatga ttcccaatat gagtcctagt actgtagcat atattgttct caatgaaaat
    28981 tttatttttt tcttttcaaa ttgtctaaca ataaagaacg tacctagaag taatattatg
    29041 aaaaaaattg ttttaaaatc tgtaatcata agaaattgtg ataaaaatga actttccaat
    29101 atttttctcc ttttatttaa aaataaattt ttctaagttt aaatgatatc aatatattaa
    29161 aaaaatgtca atattatatc acaatttact taaatagagt aaaacaagtt gattttgaaa
    29221 aatgtgatta agtacagaaa aatagggtat aataaatgtt taaagaattt gaattgtggt
    29281 aagaaaataa aaaatagctt ttataagatt tgatttcaaa ttaaaatatg ttaaataaat
    29341 aactaaaaat tattataaaa aatgttttca tagaatatat atcaaatgaa aagcttaaaa
    29401 taagtgaaga aattaactag acatcaaagg agagataaaa tgagagtaat aataattggt
    29461 ggtgttgcag ctggtatgag tgctgcagca aaattaaaaa gaataaaacc tgagtatgaa
    29521 gttgttgttt atgaaaaaac tgaaatagta tcttttggtg cttgtggatt accatacttt
    29581 gttggtggat tttttaatga tgctgatgaa ttattagcaa gaacaccaga gaaacttaga
    29641 gaagctggaa ttgatttaaa tatatttaga gaagtcgttg aggttgatag tgaatctaag
    29701 aaaataaaag taaaaaacat aaaaacagat gaaatatatg aagattacta tgataaattg
    29761 atgatagcaa ctggagcaag aagtattatg cctcctataa aaaacataaa attgaagaat
    29821 gtttctacat taaaaagttt atatgatgga gaatatctaa aaaaactttt aagtaatgaa
    29881 gataataaaa gagtgacaat aataggagct ggttttatag ggttagaagc agttgaggca
    29941 tgtaagaaat taggtaaaga tgtagatgta attcagttag aagatagaat attgccacaa
    30001 gtatttgata aggaaatgac ggatgtatta gaagaagaaa taaatagaca taatgttaat
    30061 ttgcacttag atgaaatggt tgtagaactt tgtggagaag ataaagtaga aaaagttata
    30121 acaaacaaag gggaaataga tacagatgta gtaataattg ctactggcgt gagacctaat
    30181 accgagtttt tgtcaaatac taatataaaa atgctaaaaa atggagctat agtagttgat
    30241 gagtatggaa gaacttctgt agaagatata tactctgctg gagattgtgc aactataaag
    30301 aatatagtaa gtaatgaaaa tgtatatgtt cctttagcta ctggagctaa taaattgggt
    30361 aggatagtag gagaaaacct agcaggaaga gaagtatctt atcaaggttc actatcttca
    30421 agctgtataa aaattatgga tatggaggca gcatcaactg gaattacaga aaagcaagct
    30481 aaagatttag gcataaatgt caaatctaaa tttatttctg attataatca aactcattat
    30541 tatccaggaa gaaataaaat atatgtaaag cttatttatg atgcagatac aaaagttatt
    30601 ttaggcggac aagtggctgg gtttaaggat gctgtccaaa gagcaaatgt attggcagct
    30661 gctatctttg gaaaaatgac cacaaatcaa cttggaatgt tagatttatg ttatgctcca
    30721 ccatttgcaa gaacatggga tgttttaaat gtagctggaa atgtatctaa ataatattta
    30781 gattatagct aatatagttg acaagcaatt aagggaatga atttacctaa actattttta
    30841 tcatggtaaa attcattcct aaatttttat tccaaatttt tattccaaat ttttagaaat
    30901 aaggattcca ttaaattatt catcaaaatt taaacgcaag cttaatctat cttctccaag
    30961 gatagtattt tcaaaaacat tagtagcatt ttctaaatac atactaggta tgtctaaaga
    31021 aggactaaaa tgagtcagta aaagttgttt gacatttcca agttttgcaa gattagcagc
    31081 ttctctaaaa gtcatatgtt tgtttctaac agcttttgat atatctaaat catcaccata
    31141 catggcttca catataaata aatcactatc ttttatgaaa tctggtattg attctatagg
    31201 tcttgtatcg gttgtaagac ttatcttaac tccctttctc aattctccta agaccatatc
    31261 tggagtgtat tgttttgaat ctaaaactat attttgacct tcttggagtt tgttccataa
    31321 tattttagga acttcgtttt tagttgcctt atctatatca aatttagggt ttcttctaaa
    31381 attaaaactg tatcctaaac actctataga gtgttctaaa ctgattgttg aaatttctaa
    31441 atttcttaaa actttattat ctaaagaaaa attgccttga gggttttcta taatttttaa
    31501 agtgtatggt acatattcta ctaaatttct catagaacgt atacaatcga ctattccaac
    31561 tggaccaact atagttaaat cacttgtacg accactattt ccaattgttg ataaaagtcc
    31621 taaaagtcca aatatatggt caccatgtaa atgggttata caaatcaagt ctatatcttt
    31681 aaagccacat ttttttagct tcatagatac ctgagtacct tcaccacagt caattagtat
    31741 ttttctacct ttatagttta taaatacaga ggatagaaat ctgtttggca taggtacatt
    31801 tccaccacat ccaagtaaag ttaaatctat cattttttcc cctttctaaa attaacacaa
    31861 atctgttgtt tgtttttatt atgatatcct attataatta ttttaacatt aaatataaca
    31921 ataaatgtgt atttgagtca agaaatattg tcaaataaag tttttatata gaaatgtata
    31981 aaaatttgga aaacaaaaag tgaaaattat atttaggagc atttaaagga ggaaaattta
    32041 atgaaagtat taaagaaaat tactattttg tcattaatta cattaacctt agttggttgt
    32101 agtaacggag aagataaaaa agtggttgaa ggttctaaga aaattcaaaa ttctaaaaaa
    32161 gatgaacagc ctataatagt acataaagat gcaaatatat tgaaagatag gtatgagcca
    32221 ccaaaaggat ttaaaagagt tgatgtagaa aaaggtagtt ttgaatcatt tcttaggaat
    32281 actaagctaa aaaattatgg agagaaagta aagtattatg atggaagaac taaggaatca
    32341 aaaggtattt atgatagtgt atttgatgtt gatattggca atagagattt gcatcaatgt
    32401 gcagatgcaa ttatgcttat gagagcagaa tacttgtatc agaataaact atatgatgaa
    32461 ataagttttg attttgttga tggttttaag gcgaagtatt caaaatgggc acaaggatat
    32521 agaatttcag taaaagatag tggttcaagc tggtataaag caactgaata ttcaacatct
    32581 tatgagagtt ttagaaagtt tatggatata gtgtttgcat attcaggaac gttatctctt
    32641 gaaaaagaat tggagcctgt aaaagttgag gatatgaaaa taggagatgt gtttatagtt
    32701 ggtggaagtc caggacatgc aagtataatt atggatatgg cagaaaatga aaagactggt
    32761 gaaaaggtat ttatgatagc ccaaagctat atgcctgcac aacaaacgca acttcttatt
    32821 aatcgtaaaa atccaaattt gagtccttgg tatagtttag attttgaagg tgatttgaca
    32881 atacctcaat ggacttttaa aagagaccag ttgaaaagat tttaaaataa taattttata
    32941 taataaaaga aaaatataga tgtgaaaaaa tgtaattaga ttaaaactaa ttggatatgg
    33001 acaaaattaa attgatagat aaatattaaa aattacacta attaatctta ctatagttta
    33061 atatttatct aaaaagtttt atgtaaaata taaattttat attaaagcta aaactaattt
    33121 gaggaggcta atttatattg aagattagaa ggtatttaat tttggtaatt ataattctaa
    33181 ttacaacaag tcagagtttt atttcttatg ggctagagcg atttgaagaa ataaccattt
    33241 ttcacactaa tgatatacat ggaagatatg ctgaaggtga tgaccatata caaataggaa
    33301 atctagccac attgaaaaaa gaaactccaa actcaatttt agtagatgca ggagattgtt
    33361 tgcatggact tccaatagta aatatggata gagggaaaag tgcaattgaa ttaattaaat
    33421 gtgctggata tgactatatt actcctggaa atcatgactt taattatgga aaagatagac
    33481 tgtttgagtt atctaaaagt gctaatttag gagagaatac tttaaaattt ttatcatcaa
    33541 atatttttga aaatggaaaa aaagtttttg atagtaatga tataaaagaa atagatggaa
    33601 taaaaatagg attttttgga ctctcaaccc aggaaacaaa gagcaaaact ggatttaaaa
    33661 atgtggaagg attagagttt aaaaatccta tagaaagtgc aacagaacaa gtaaatgaat
    33721 taaaaggaaa gggtgccaat gtaatagttg caatatgtca tattggtaca aatagtttaa
    33781 gtaagccaaa tagtatagat atagcaaatg aggttcaagg aattgatttg attattgatg
    33841 gacacagcca cactaagttt gaaaatggta aacaagtggg tgatacattg attgtaagtg
    33901 caggacaata tttagaaaat attggcgaga taaaactaac tttggatact aatgatgtag
    33961 aaaatataaa aatagaaaat aaatcagtta gattaattgg aaaagaagaa gcattaaagt
    34021 ataagcttga ctccgctgta agtaaaaagg ttgatgaaat aaaagaacaa caagaaaaaa
    34081 tattagataa agttataggg gtaactacaa atactctaga tggttcatat gaaaatgtaa
    34141 ggacaaaaga aactaatttg ggtaatttaa taagtgacat tttactagat aaaactaagg
    34201 ctgatatttc tttatttaat ggagggaata ttcgagatac gatagaaaaa ggcaatataa
    34261 caagaagaga tattgtagat gtatttcctt ttagcaatac tatagttacc aaagaactta
    34321 caggagcaca gattaaagat gtattggagc atggagtaaa actttatcca gaaaaaagtt
    34381 cagccttttt gcaagtggga ggaatttcat attactttaa tcctaaacaa aaagaaggcg
    34441 aaaaaataac aaatattcaa aaagaaggaa agccattaga tttaaataaa aagtatgtag
    34501 ttgcaacgaa tgattatata gcatcaggtg gagatgaatt tccttgtttt tcaaaagagc
    34561 cagtactaaa ggaatttgga aatttagaga gtgcagtcat tgaatatata gagtacaaaa
    34621 aagaaatatc aaaaaatgta gatggaagaa ttggtattaa atctaaagat gagttaataa
    34681 gcaagaatca tgagagtgat ttgatattaa gtgaaaatga aaaaaatatc aatgataatg
    34741 aagagaatct atctaataat ataaaaagct ctgatagtga taaaaataaa agcttagata
    34801 aaacaagtgc tagagaaagt tctaatctag gaatatctaa aagagaagct aaagaaaaat
    34861 ctcctaaaac aggagattta ggtttttcaa atagtataat tatatttata gtttcaagta
    34921 cattgatatg tttattgaat tttaatcaaa aagagttaaa ggataaaaag tcaaaataaa
    34981 aattaatgag caaaaattag tagatagtaa tttaatttta gataatagta aacagcaaag
    35041 gaaattagtt atataaatac taatttcctt tgttttatgt tttaacatga ttacttttaa
    35101 ataaaattgt attttatgaa aaataattac actaaatggc atagtattat ggggattttt
    35161 agtttttatt caagtaaatg aaatcatatt aacaacttct gatatattta caaattgata
    35221 aaaaaatgat tttattagta tgatataatt tagctagtat atatgatgat ggaggatata
    35281 gggatgaata atatattact tttggaagat gacaaatctt taaatagagg aattagtttt
    35341 aaattaaaaa aagaaggata caatgttttt agtgcatttg gtatagaaga agcaaaaaat
    35401 attttcgcaa aagaagagat atgcttaatt atttccgata taggtctacc tgatggtagt
    35461 ggttttgact tttgtgagga gattagaaag aaaagtgatg tttacataat aatgttgaca
    35521 gctcttgatg aagaggtgga tattgtcaca ggatatgatt taggtgcaga tgattatatc
    35581 acaaagcctt ttagcttgat ggtattaata tcaaaagtga atgcgcttat gaaaagagta
    35641 aatacagtaa aaaattatac tctattagtt tgtgatgatt tattctttta ttatatagaa
    35701 aataaattaa ttataagaac agatgataag gaagaagaga taattttaag taagacagag
    35761 acaaaactat taaaatattt gatggaaaat tctatgcaga cattgactaa agagcaactg
    35821 ttagaatctt tatgggatag tagtggtaat tttgtagatg ataatacgat tgcagtaaat
    35881 ataagaagac ttagacaaaa ggttgaaaaa aatccatctg aacctaaata tataaaaaca
    35941 gtaagagggg ttggatatat gtggggagaa aggagtataa aaaaatgcta aaaaatatga
    36001 acctcaaaga tggtttaagt gtgaaagatg attatataat gagaagagtt attgtatatt
    36061 ctgttttatc tgtactaata gtaacccttg tatttatact ttttgaatat agcagattac
    36121 ataaattgta tgaacatcaa ataaaaaaca ctcaaaatat tgctggaata ttgattgaaa
    36181 aatatcctaa tgatgaaata gatatagtaa attctattta taattctgat tatagtaagg
    36241 tgtctactgg agaaaaggca tttaagaaat ttggctatgg attggaaaat aaaatgtcaa
    36301 atgataaaaa ttttagctta tacttaaaat ctttttttaa ggaatcattt tttatttttt
    36361 taatgatgtt tatagtagtt ataggtataa tatattattt tataagatac ataaataaaa
    36421 gattgagtaa aatttatttt atagtagata agatgtcaca agaaaactac ttgaaggaca
    36481 ctactgagtc agaagaaact ggttatctag tagaatataa tgaatacttt aaagaaggca
    36541 tatttagtaa gataaataac tgtctatatg agcttaatag aagtttaaag ataaagttta
    36601 ttaaattaga aaaagaaaaa gaaagtgtaa aatcattagt aacagatatt tcccatcaat
    36661 taaaaactcc tttagcatcc ttaaaattat acaatacatt acttatagaa gaagaattag
    36721 aagaagatga agccaacgag ttcttattaa ccaataaggc ttctataaat aaattagaaa
    36781 atttaataaa ttctttagtg aacatatcaa ggttagaaat tagcatgata aacataaaaa
    36841 aagaagataa tgatataaaa aatactattc taaatgcagt aaaaagtgtt acccctaagg
    36901 ctaagagcaa aagtataaca ttaaatttaa atgaatttaa cagtagaatt ataccacatg
    36961 ataaaaagtg gactgaggag agtattttta atgtattaga caatgctgta aagtatacac
    37021 aggtaaatgg agaaataaat atatctgtag aagaaactgc taatttcttt aaaattataa
    37081 tagaggataa tggcataggg attgaaaaaa atgagtttaa taatatattt aaacgttttt
    37141 atagaggttc tgctgatgaa attgaatctg tagaaggtag tggggtagga ttgtatttaa
    37201 gtagaaagat tttagaagaa caaggtggaa atattatagt ttcttcaaaa gtaggaatag
    37261 ggagcaagtt ttctttgttt cttacaacta tgtaatatta aatgaaagga aaatgtaagc
    37321 ctctacattt attatataag tataaacagc tccagaaata aaaaattaat aaaatataga
    37381 gtaatataac ttagaatgag aggataattt tatgaaaata ctatatactg aaaatttatc
    37441 aaagcattat ggaaaaggag aaagcttagt aagagctttg gacaatgttg acttagaaat
    37501 aaatgaaggt gaatttgttg caataatagg aaaatcaggt tctggaaaaa gtacactttt
    37561 gcatatgatt ggaggtcttg atatacctac atctgggaag gtgtatatag ataataaaaa
    37621 tatatttaca ttaaaagaag aagaacttgc tgtatttaga agaagaaaaa tagggtttat
    37681 atttcaaagc tataatttaa ttccatctct taatgtatgg gaaaatgtag tgttgccaat
    37741 cggtttagat ggtagagaag tagatgaatc ttttataaaa gaacttttaa aatcattagg
    37801 acttgaaaat aaacacgacg ttttaccaaa tacattatca ggtggtcaac aacaaagggt
    37861 ggcaatagca agagcattag ctacaagacc agctataata cttgctgatg aaccaactgg
    37921 aaacctagat tctaaaacgt ctgatgaggt tatgagtata ttaaaatcta tgtctaagaa
    37981 gtacagtcaa actctagtta tgataactca tgatgacagt atagcccaaa tggctgatag
    38041 agttatattc atagaagatg gcagggtgtc aaaggttggt gataaaaatg attaatacta
    38101 ctaaaatagc aaaaagtaat ttaaagcaaa ataaatcaaa aagtattcta ataataatta
    38161 ctataatact ttcaacctta atgctttctt ctattggaat ttatattgtt gatgcaggag
    38221 catatcaaaa agaaaatact ataaagtatt caggcaatta tcaaggtata ttagcaaatg
    38281 tagatgaaaa acaggctgat atattgagta atcatgcaga tgttgaatta actggtgaaa
    38341 tgaatggagt aggtgttgaa aaactggaag atgattcaaa tatttctcta gcatacatga
    38401 atgaagatgc tttgaaatta aatagttttg aatttatcaa aggaaaattg ccatcaaaag
    38461 agaatgaaat tgtgttagat agtggagctt taaaggcact tggttataaa aataaagatt
    38521 taggtgaaaa gataaaaata tcttatgatg actataaaaa tgataaaaaa attgaaaaag
    38581 agttcattat aagtggaatc ttaaaaacta gcgaaatatc tgaagcaggt aaatattatt
    38641 atgctattat atcagaatca tatatgagaa atactagaga tatgtcacaa gaagatttta
    38701 atatttatgt gaaatttaat gataaaagta atttatcaat agagcaagca aaagaaaaat
    38761 tagataaaat tgctaatgat ataggactgg atactattaa tactgctgta aatgaaaatt
    38821 atataaatgc tttaaaacca gatatggaaa cgattatggg tggagttttt gtagggttag
    38881 taattgtatt atctagtatt ttagtaatat acaatatatt ttatatttca atagttacaa
    38941 aggttcaaga atttggaaaa cttagagcaa ttggtgcaac taagaaacaa ataaaaaata
    39001 tagtgtttaa agaagggttc atattagccg gaatagctat accaatagga ataattttag
    39061 gctatgtact tgcaaatatt ataataaaat catttatgga tatagatgta aaatcatcac
    39121 gattaccagt tatattatcg gttgtggtaa taagctttat atctgttgtt ttatcacttt
    39181 taaaaccaat gaaagtagca tcaaaagtat ccattgttga cgcagtaaga tatacaggaa
    39241 ataaaataag taataagagt aagagaaaag gatataaaaa tataaatcta aagagattat
    39301 cacatgccaa tttagaaaga aataaaaaac gtacttacat gacattggca tcacttatac
    39361 ttagtggaac tatatttata acagtatcaa ctgccttaga aagttttgat gctgagaaaa
    39421 tggcaagaga acactttcct tatgatatag aagttagact atctggttat gaaatgaaca
    39481 gtgataaaaa tcctaaagat aatttgaata ttttacaaat ggataatcct ctaagtaaag
    39541 atttttttaa tcaaataaag aacatggaag gaattaaaag aatagaatct gcaagaagtg
    39601 taaaaattgg tatggaggac tatgatgttg aatttaagta tgacctttta caaagtataa
    39661 atgaaaatga tgtaaaatca ttaaacaaga atataataga tggaaaaata aatcttgaaa
    39721 gacttcaaac tggagatgaa atagtaatta ctcatgttga tacagcaaaa gaaatgggag
    39781 taaaagcagg agataaaata agattaactt tatatgatgg agataaaaag ataaaaaaag
    39841 aatttaaagt tcaagcaata gctatggggg ttccaagttt tggtataggg aaagatttta
    39901 tagatagaac tttgaaatac gattcaacta gtagcttggg aatatataca gaagaaggaa
    39961 agtatcaaga aatcaaagat agtatcaaga agattgctaa aaataatgga tttttagaga
    40021 ctgattttat agatagtaga attgagtcaa ataaggctac tatttcattt ataaagataa
    40081 tgggatacac gctaacaggc ataataggtg tgattggatt tatgaatctt gtcaacacaa
    40141 tgataactag catagttact agaaaaaaag aacttggtat gcttcaagct attggtctta
    40201 ctaataaaca attagtgaaa atgcttaatt cagaggcaat atattacact tctggaatga
    40261 tgataggaag tattctattt ggaggtatat tagggtatat tgctgttata tttctaaaaa
    40321 agactggatt atcttatgca acatattctt tgccaatagt accaatcttg ttgatgattg
    40381 tgtgtattct tatagctcaa tttataacaa cttatttgat aggacgaagt tttaataaag
    40441 aatctcttat tgatagagta agatacagtg aataaaatgc aaatatttat ggttagttta
    40501 actagcaaaa aacatataaa aaaatatagt taaagagatt cataattaaa taggttaaag
    40561 catagtacag agcaaacaat ttaaacagat gtttgctctg tattttatta tgtaatcaat
    40621 aatatatcta atatactgat attattcatg ttagaaaatg taccggtaca attctaaaaa
    40681 agtataaaat tgtaccatat aaaatgaaaa ttttgtataa taatcgtata gaaaataaat
    40741 aggagctgat atatatgaat aagaagatta attatgcacc aggaccaact gaaactaggg
    40801 aaaatgtaag attagcaaga gcagaaaaaa caacaaatcc agatatagat attgattttg
    40861 ttgaatttta taaaaagaca tgcgagaagt ttggtaatat aattggaaca aaaaacgatg
    40921 tttacatatt gagtggtgaa ggaatattag gtcttgaagc agcatgtgca tcacttacag
    40981 aaaaaggaga tagagtactt gtaattgaca atggtatata tggagaaggt tttaaagatt
    41041 ttgtaacaat gtatggtggg gagtatgtgc ttttttcaag tgagtatact aaaagtatag
    41101 atattgatga attgaaagca tttttagatg aggatagtaa ttttaaatat gcaacagtag
    41161 tgcattgtga tactccaact ggcgtattaa atgatgtaag caaaatatgt ccattactta
    41221 aaaaatatgg aatattaact gtagttgatt ctgtagcagg tatggtagga gaaagattaa
    41281 gtgtagatga atctaaaata gatataatat taggtggttc acaaaaagct atttctgcac
    41341 cagcaggact tactatagtc ggaataagtc aagatgcaaa aaattgtata aaaaatagaa
    41401 aaactgatgt aataggtttt tattgtaatt taaatatatg ggaaggctat tatgagaaaa
    41461 aatacttccc ttatacaatg ccaataagtg acataatggg gcttgataaa gcacttgata
    41521 atatattaga agaaggaata gaaaatgtat taaatagaca tgaaaagata gcttatagtg
    41581 ttagaaaatc tattgaggaa tatggtctag aactattttt agaagaagga tattctaata
    41641 cagtcacagc tataaaaatt cctgaaagta taggagcttt agaacttact gattatatgc
    41701 ttaaaaatta taatacactt gttgcaacat cactaaatca atatatggat actattttaa
    41761 gaataggtca tatgggagag aatgcaaatc taaataaaat agaacatgta ctaaatgttt
    41821 tggataaaag tttaagtgca ttaggattca aaggaaatgg aaatttgtta aatttattta
    41881 ataagtatta tttttaatag atagggggat atagtatgga agttacagct aagcaaaaat
    41941 ttacaacgaa agacatggtg gaaacctcac tattaatagc attagtattt attgcaacta
    42001 aatttataaa cataagatta ccaatatcta taaatggtgg gcttgtgcat ttaggaactg
    42061 caatgttgtt catatcagca atagtatttg gaggtaaaaa aggtgcttta tcaggggcta
    42121 ttggtatgtc actttttgat ttgatatctg ggtggacttt atgggcaccg tttaccttta
    42181 tagttagagg agttatgggt tatttacttg gaaagattgc atgggcaaat ggtaataatg
    42241 ggaataattt tttaatcaat gtaataggaa tatgtgtttc atcaatttgg atgctatttg
    42301 gatattatgt aacagaagtt atactttatg gtaattttat agtacctctt acttcaatac
    42361 caggtaattt aatgcaagta ttgataggtc ttataatagc gcttcctatt tctaaggtat
    42421 tgaaaaaatg tataaaataa atataaagtg aactaaaatt tatgatagca taaattatac
    42481 tgtataatat taggatggag tacataatta aaaaatttta gtatagaggt tagttcacaa
    42541 tgttatcagt tacaagatat tcaatcaaaa atgttcgttt aaagccgtgg gtaaagttta
    42601 tttggtattt tgagacaaaa tcaaatatac tgttaaataa taagttactt ccgacagaca
    42661 gtatagatat tattctcaat ctttctgatg ttatggaata taaaattgaa aatcaagact
    42721 atactgctag taatatgcat tttaatggaa taagggataa acatggattt attattcaac
    42781 atggaaatat acgtgttata gggatttctt tttatccatt tggtctctat ccatttttaa
    42841 agataccaat ttcagagttt aacagacaaa ttgttgacct agaagcagta tcacagctat
    42901 ttgcaaaaaa attggaagag tcattaaatc caacacaatc agtagaaaaa attgttttat
    42961 atttagaaga agtattatta tccatattag aagaagattt aatttcaaat aaatatgtaa
    43021 agcttctaaa ttcatttata tatgttaata gatatagcaa tataaaagca ttttgtgatg
    43081 atacaaatat aaatattaaa acattggaaa gaatctgttt aaaatataca gggtatacac
    43141 ctaagatttt aaagagaatt tacagattta aaatggcaag caatcaactt atttataatt
    43201 ataaagatga tgaacttttt gattttattt atgaaaatga gtactatgac caagcacact
    43261 ttataaaaga atttaaaaaa ttttcaggga cgtcaccaat taaatttatt ggtgagaata
    43321 agacgattaa agaaaatata aagtacagct atttataatt atactgtcga ttttttacaa
    43381 tacctccaat ttattttttg atatgatata ggaaaataaa ttggaggttt tacatatgaa
    43441 aattattaca gaaatagttg agtttaatgt ggaagaaaat ttatcaaaag aagagtttat
    43501 agaaattgta aatgacttag aattaaaatt tcattctatt caatctggtt ttattgatac
    43561 tgagctttta tatgatgaaa agtctcaaaa gtggattatg atacaacatt gggaatctat
    43621 agaagacctc aagagtgctt ctagtaaaat gtttaaagat tcttcaactg agaaatttag
    43681 aaaagcttta aagaatcaaa ctgttaagat gaatataata ccacaaatta aaagttggaa
    43741 attatagaac ttaatgtaaa agatagttaa atagtatcaa actaaattag ccaaataaca
    43801 ttttgtattt aaattgttat ttggctttta gtgtttattt taatatgagt gttatatact
    43861 agaaaaacca accttaaata ctactatcta aaagcactat tgctgtacga gaggcaatag
    43921 gcattttaga agaaggagga gttgagctaa agtatacatc ataatctctt aagttttctt
    43981 tttttgtgtt tcctccaaac tctaccatat cagtatttaa tatcgttttg tagtttgtat
    44041 tttgaaactt tgaaatatca actgattgta ttagattagg gtggaaatta aatataaata
    44101 aatatttgcc cttactgtac accaaaatct ttttatcatt atccatatgt ataagttcat
    44161 gactattttg gataaaaata tcactgtatt ttgtaagttc gagcatagat ttatcaaaat
    44221 ttaataattg tttatacttt aagttgttat cttctgaaag gctccattgt cttctggcat
    44281 acttatagct ccaattgttg ccttctcgtg gaaaatcaat ccactcagga tgtccaaatt
    44341 catttcccat aaaatttaga tatccttcac ctgctagaga aaaagttatt aatttaatta
    44401 atttaataag agatattgct ctattgataa catggttatt tgagtttatt tccatattcc
    44461 agtacatttc cttgtcagct agccaaaaaa taattgtttt atcgccaact aatgcttggt
    44521 catgggattc acaatatcct atattttttt ctccaggtcg tctagtagta agttcatacc
    44581 acatttttcc caaatcccag tcttcatcag ataagttaga gatagttttt atccaaaaat
    44641 caggtactcc cattgcgagt ctatagtcaa atccaattcc gccgtctttt atagggatac
    44701 acataccagg cattccactc atatcttctg caatacttat cgaattaggt ttaatttctt
    44761 ttattaattc atttgcaaat tggagatatg ttatagcttc aatatctgta ttcatactaa
    44821 aatatttttc ataactatca aatgaaactc caagaccatg gttatgatat aacattgaag
    44881 tcacaccatc aaacctaaag ccatcaaaat ggtactcatt taaccaaaat tttatatttg
    44941 aaagcaaaaa atgtattact tcaggtttac catagttaaa aagttttgta ccccaagcag
    45001 gatgatttcc ctttgaacct gagtggaaaa attgatgttc acttccatca aactcattta
    45061 ttccttctaa agtattcttt acagcatgag aatgaactaa atccaataaa acagcaatcc
    45121 ccattgaatg agcagtgttt atgaggtttt ttaagtcttc aggagtacca aacctagatg
    45181 agattgcata aaagtttgaa acttggtatc caaaagatgc ataatatgga tgttccataa
    45241 tagccataag ctgaatggta ttgtatccag ctttttttat cttaggaagt attttttcag
    45301 taaattcatt ataagttcca atagattcgc tttcagtaga catacctata tgacattcat
    45361 agattagtgg ataagttatg ttttttaagt caaaatcatt gtcagtccag ataaatggag
    45421 tttttggttg ccatatttgt ccattaaatc caccaaaatc tttttgaatt actcttttta
    45481 tatataatgg tatacggtca aatgtttttc catttgcagt aacttgtacc tttacctctg
    45541 atttgtgagg taagctattt tgaccaggaa taaatatttc ccaattacca gacccaattt
    45601 ttcttagagg atgagatttt ctattccaat tattaaagtc accaattagt gacaaactat
    45661 ctgcatttgg agcccattcc ctatagaaga atccatcatt tgtctgatga aatccatagt
    45721 aaagatgagc atttgcaaaa gaactaaaat ctttatagtt tttcaatgtt tttgatttca
    45781 tattctcata atgtttcatt cgctttttta tatcagattc aaatggttgt aggtatacat
    45841 caatatctaa aattttaaat cttttttgac ttttcattgt atttctcctc cataataaat
    45901 attacatcta aaaaataata tttatctcat actatcttct ctatataatt gatatatatt
    45961 atataatcct aataaaaagc ttaagccacc aatgattaat ttcataattt acctccctag
    46021 tatttatact tatatatcgt attcatcaaa atttaaaagt ttgtttaaaa ctcttttaat
    46081 aatatcataa tcaaagcctt tataggaaag atgttgagaa attttttgat atattttctt
    46141 tttatcttct ttctttactc tttcatacct tttttttgct agatacatag cattttcaaa
    46201 ttcagtgtcc ttgtctaatt cagatactgc ttcattgatt gtacttctat tgataccttt
    46261 attatataaa ttctgcttaa ttctgttttt gccacattta tttaggttaa catttgtgtt
    46321 aacaattttt tgagctaata cactgtcatt tacgagatta taacttttaa gaaaatctat
    46381 gaccctctca attatatttt catcaaattc tgatgatagt ttttctttta attttttttc
    46441 agattggtca gcatgagata aaatatttaa tgctttattt ttagctttta tatacatttc
    46501 atcatctaaa ataggtttta attcttcttc atttatttca ttcccctttt ttaaattaaa
    46561 tgtataaaca agttctttaa atatagcaat aaaaaattta tcatctacat aaatattaac
    46621 tctatcatca tttctttttt gttgttctat ttttgtaatt atacccataa gtcctccttt
    46681 tttatatgta attatattaa caaaagc
//
LOCUS       DAEMGG010000015        46582 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEMGG010000015 DAEMGG010000000
VERSION     DAEMGG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.015"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(277..1527)
                     /locus_tag="KPK24_000866"
     CDS             complement(277..1527)
                     /locus_tag="KPK24_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG2298573.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1560..2501)
                     /locus_tag="KPK24_000867"
     CDS             complement(1560..2501)
                     /locus_tag="KPK24_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBG2298574.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2741..3817)
                     /locus_tag="KPK24_000868"
     CDS             complement(2741..3817)
                     /locus_tag="KPK24_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG2298575.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4190..4705)
                     /locus_tag="KPK24_000869"
     CDS             complement(4190..4705)
                     /locus_tag="KPK24_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG2298576.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(5068..6138)
                     /gene="leuB"
                     /locus_tag="KPK24_000870"
     CDS             complement(5068..6138)
                     /gene="leuB"
                     /locus_tag="KPK24_000870"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG2298577.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(6145..6636)
                     /gene="leuD"
                     /locus_tag="KPK24_000871"
     CDS             complement(6145..6636)
                     /gene="leuD"
                     /locus_tag="KPK24_000871"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG2298578.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6651..7928)
                     /gene="leuC"
                     /locus_tag="KPK24_000872"
     CDS             complement(6651..7928)
                     /gene="leuC"
                     /locus_tag="KPK24_000872"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG2298579.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAKLKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAIIGKISKPSEII"
     gene            complement(7885..9627)
                     /gene="leuA"
                     /locus_tag="KPK24_000873"
     CDS             complement(7885..9627)
                     /gene="leuA"
                     /locus_tag="KPK24_000873"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG2298580.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9751..9997)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10145..10591)
                     /locus_tag="KPK24_000874"
     CDS             complement(10145..10591)
                     /locus_tag="KPK24_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298581.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTNESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10612..11331)
                     /locus_tag="KPK24_000875"
     CDS             complement(10612..11331)
                     /locus_tag="KPK24_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG2298582.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11449..12723)
                     /locus_tag="KPK24_000876"
     CDS             complement(11449..12723)
                     /locus_tag="KPK24_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG2298583.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(12923..14290)
                     /locus_tag="KPK24_000877"
     CDS             complement(12923..14290)
                     /locus_tag="KPK24_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2298584.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(14540..15004)
                     /locus_tag="KPK24_000878"
     CDS             complement(14540..15004)
                     /locus_tag="KPK24_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG2298585.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(15009..15638)
                     /locus_tag="KPK24_000879"
     CDS             complement(15009..15638)
                     /locus_tag="KPK24_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG2298586.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRIIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNKFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(15672..16559)
                     /locus_tag="KPK24_000880"
     CDS             complement(15672..16559)
                     /locus_tag="KPK24_000880"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG2298587.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(16588..17826)
                     /locus_tag="KPK24_000881"
     CDS             complement(16588..17826)
                     /locus_tag="KPK24_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298588.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(18210..19352)
                     /locus_tag="KPK24_000882"
     CDS             complement(18210..19352)
                     /locus_tag="KPK24_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG2298589.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(19493..20359)
                     /locus_tag="KPK24_000883"
     CDS             complement(19493..20359)
                     /locus_tag="KPK24_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2298590.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(20693..22240)
                     /locus_tag="KPK24_000884"
     CDS             complement(20693..22240)
                     /locus_tag="KPK24_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG2298591.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(22233..23369)
                     /locus_tag="KPK24_000885"
     CDS             complement(22233..23369)
                     /locus_tag="KPK24_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298592.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(23632..24729)
                     /gene="dinB"
                     /locus_tag="KPK24_000886"
     CDS             complement(23632..24729)
                     /gene="dinB"
                     /locus_tag="KPK24_000886"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG2298593.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVDVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(25119..26624)
                     /locus_tag="KPK24_000887"
     CDS             complement(25119..26624)
                     /locus_tag="KPK24_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG2298594.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(26886..27236)
                     /locus_tag="KPK24_000888"
     CDS             complement(26886..27236)
                     /locus_tag="KPK24_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298595.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(27459..27650)
                     /locus_tag="KPK24_000889"
     CDS             complement(27459..27650)
                     /locus_tag="KPK24_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298596.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(27768..28682)
                     /locus_tag="KPK24_000890"
     CDS             complement(27768..28682)
                     /locus_tag="KPK24_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2298597.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTNESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            complement(28816..30312)
                     /locus_tag="KPK24_000891"
     CDS             complement(28816..30312)
                     /locus_tag="KPK24_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2298598.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            complement(30592..32082)
                     /locus_tag="KPK24_000892"
     CDS             complement(30592..32082)
                     /locus_tag="KPK24_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2298599.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            32549..32749
                     /locus_tag="KPK24_000893"
     CDS             32549..32749
                     /locus_tag="KPK24_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2298600.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            complement(32915..33793)
                     /gene="speB"
                     /locus_tag="KPK24_000894"
     CDS             complement(32915..33793)
                     /gene="speB"
                     /locus_tag="KPK24_000894"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG2298601.1"
                     /translation="MKNNFYHMNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(33783..34634)
                     /gene="speE"
                     /locus_tag="KPK24_000895"
     CDS             complement(33783..34634)
                     /gene="speE"
                     /locus_tag="KPK24_000895"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG2298602.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            complement(34664..35077)
                     /gene="speD"
                     /locus_tag="KPK24_000896"
     CDS             complement(34664..35077)
                     /gene="speD"
                     /locus_tag="KPK24_000896"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG2298603.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            complement(35164..36639)
                     /locus_tag="KPK24_000897"
     CDS             complement(35164..36639)
                     /locus_tag="KPK24_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG2298604.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            complement(37277..37930)
                     /locus_tag="KPK24_000898"
     CDS             complement(37277..37930)
                     /locus_tag="KPK24_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2298605.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPIAEMKKEKLL"
     gene            complement(38175..40040)
                     /locus_tag="KPK24_000899"
     CDS             complement(38175..40040)
                     /locus_tag="KPK24_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBG2298606.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(40045..42465)
                     /locus_tag="KPK24_000900"
     CDS             complement(40045..42465)
                     /locus_tag="KPK24_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG2298607.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     TEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(42479..43921)
                     /gene="glgA"
                     /locus_tag="KPK24_000901"
     CDS             complement(42479..43921)
                     /gene="glgA"
                     /locus_tag="KPK24_000901"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG2298608.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(43950..45080)
                     /gene="glgD"
                     /locus_tag="KPK24_000902"
     CDS             complement(43950..45080)
                     /gene="glgD"
                     /locus_tag="KPK24_000902"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG2298609.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(45082..46242)
                     /locus_tag="KPK24_000903"
     CDS             complement(45082..46242)
                     /locus_tag="KPK24_000903"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG2298610.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT        14984 a         8390 c         4755 g        18453 t
ORIGIN      
        1 tttattaact tttttataat tataataaaa atatataaca gtactattat aaatacacat
       61 ttaaaacctt ataaatatac aaatacaatt atatgaattt tcatcctcaa ttatagcata
      121 gaatatgact atctatatcc taaaattatc catttctaaa aaaaaggtaa ctattattaa
      181 gaatatcaac ttatagaatt tatattccac atacgatttt caatcaaaaa aacaagggct
      241 aaaaagtgaa ttgtgcactt tttagccctt tattaattat tcatttattt catgtaagaa
      301 tagaccacat cttactttag gttcaaacca tgttgactta gctggcatta tcttatttgc
      361 atctgctact cttattaaat cttcaatact agtaggatac attgcaaatg ctactttcat
      421 atcttcatta actctttttt ctaattcttc aagacctcta attccaccga taaaatcaat
      481 tcttttatct actctaggat tatcaattcc taaaatagca tcaataacat agtcttgtaa
      541 tatagaaatg tctaatgagt caacagggtc ttctatttct aataaatttt tatttgcttt
      601 cattttatac cacttatcac ctaggtacat tccgaaagtt ccttttttgt caggtttata
      661 tttttctttt ccttctattt cttctagttc aaaattttct tttattttat ttataaattc
      721 ttcctcgctt aaaccattta agtctttgat aaccctatta taatccaaaa ccattaaatt
      781 ctcatctggt gcaatcattg ctatgaagta attaaattct tcttttccag tataatttgg
      841 atttttagct ctcatttctt tggctacatt ttctgctgaa gctgtcctat gatgaccatc
      901 tgcaatgtat agataatcta atttttcaaa tttatctact aggttatcta ttatatcatc
      961 attatcaatc ttccatactg tatgctctat cttgtcatct gttatgaaat catataaagg
     1021 ttcattatta tccataaaat cattgattat atcatctatc accctttgat gtttgtaagt
     1081 tacaagtata gttcccgtat tagcatcaca gtattttatg tgtttagttc tatcaatttc
     1141 cttttctggt cgagtgtact catgtttttt tattgttcca ttcaagcttt catcaactga
     1201 tatacatgct actataccaa cttgagctct accatccatg atttgtttat atatatatat
     1261 acactctttg tcatctttta ctagaatttc ttgttttcga aactcatcta aattttctcg
     1321 cgcttttaaa taaacttttt catcatataa atctatattt tcatctaaat caatttctga
     1381 tttatctata tgcaaaaatg aatatggatt cccttctgca attcttctag cttctttact
     1441 attcataaca tcgtaaggaa gtgctgctac tttatctact atatatttat ttggtctaat
     1501 cgccttaaat ggtttaatcg ttgccattac taatttcctc cctaattata caaaatattt
     1561 tataatgcta cccctattag attgttgaag tcaagaaaga aatcttgaat cacatttact
     1621 atttcgtctc ctattctttc ttgagcttct actgtagatg ccccaatatg aggtgtagga
     1681 gaaactcttg gatgatttac taagtctaaa tttaatgtag gttcttgttc ataaacatct
     1741 atagcagctg ctgataattt accttcatct aatgctttta gtaaatcttt ttcatcaact
     1801 aaaccgcctc ttgcacagtt tattaaataa gcccctttct tcatcttatt tatttctttt
     1861 tcagttatta ctgccccctt attcttatca aatggtatgt gtaaagataa gaaatctgca
     1921 ttttctaaaa cttcttccat atcacagaat ttataattgt taaagccttg cgcctcaccc
     1981 attatgtcag tatatataac attcatacct aataattctg ctctctttgc aacttctttt
     2041 gcaattcttc caaaacctat aagtccaagt gtctttccat taatttctgt acctttatac
     2101 tttttcttct cccattttcc atctctcata gttacatttg cagtatttat gaatcttgca
     2161 agaacaaata attgacctat tgtaagctct gcaacagata tactgcttgc atttggagta
     2221 ttcataactt ttatgccatt agcttgagca tatttaacat ctatattatc cagacctact
     2281 ccacctctta ctatcaactt caacttttta ccctctaatg ctttatctat tacatcttta
     2341 gtaactttag tagctgaacg tactacaagt acatcaacat ctttaagttt atctcctaaa
     2401 acatcttttt cataaaactg ctctatcaca ttaaaatcta actctctcaa ttttctcgct
     2461 gcttctttct ctattccatc agtaactaat atattataca taacaaactc ctccaatttt
     2521 tattcactta atttttccaa tttttatttt ttaaagtatt ttcatcaata aaaaacttta
     2581 cttaatttaa ctttctgttt atataatttt caaaatattt ttttattaaa gtcatatcaa
     2641 attttcctat tgtttgttaa aaatttaata tgtaattttt ataaaatatt atttattata
     2701 tattctaaat ttatcttaaa acatacatat ttttacaata ttatttatta aaacctaata
     2761 tatcatcaat attatctaat aattcttgta catcttcgac agtatagtca cccatatgtg
     2821 aaattctaaa agttttttct tttaaatctc catagccatt tgctatttgg aatcctcgtt
     2881 cttgtaattt tgaatttaaa tttgatacac taataccttg agtattttct ataacagtaa
     2941 gtgtattgga taagtgattt tcattggtaa aaatttggaa gtgtttttta gcccagtttc
     3001 taactaagtt tgccatgtct tcatgtctgt tgaatctgtt atctaggcct tcatctaata
     3061 tattatctaa ttggaaatct aaagcgaaca tgtgagaaag agatggtgtt gaagggtatt
     3121 ggtagttttt cttgattaag tattcataca ttgctaacaa atcaagatat actcctctaa
     3181 aaggtacttg cttagctcta tcgattgctt tttgagaaaa tgtgcaaata gacattcctg
     3241 gtggtaatcc cagtgctttt tgtactgatg ttatacaaat atctattcca atcttatcaa
     3301 cctcgacttt tattccaccg gctgaactta cagtatctac acagtagatt acatcttcat
     3361 actttttaac aacttctcca atttcttcta ttggatttct tattccagtt gaagtttcat
     3421 tgtgagttac agttattaaa tcgtattctc cggtagaaag caccttatca accatttctg
     3481 gcgttgttgc ctctcctaat tcaactttaa atatatccgc tgggacatta ttagccactg
     3541 ccattttgta ccatctatca ccaaaggatc cacaggaaaa tactgctgcc tttttggatg
     3601 tacaggaacg aatggagcct tccattaaac cagttccgga tgacgtggat aaaagtatcg
     3661 tgttgtttgt atagaatagt ttttgcatct tttcacttat actcttttgg agcatagatg
     3721 catcttttcc tctatgtcct atcattggtg tagccatctt ttgcaagact tcttctttaa
     3781 catcaatagg accaggaata aataatttct tactcattat aattcccccc tttttagttt
     3841 gtaatatgga tagcgttttt actgtcctta tttttaataa ctataatatc aagctgaatt
     3901 aactatgtca acataaaatt tattttttat atataattta ttttatatat tttatgaaaa
     3961 cattcatttt ctaccttttc ataagtctga attttctgaa ttattctctc aaaaaaaata
     4021 tactttttat ctatataaat taattttttt agtatgtaaa tttgacgcta ttacgataaa
     4081 tctttttagt ataactaatt tatttttatc ttgtattttt attgtatttt taaattttat
     4141 tttttctaaa tataaaattt atgttttaaa atgtaaaatt tttatttaat caaagttcaa
     4201 tatattccac tttttctttc gattcttcaa aaaaccattc ttcattagaa aaatcacctt
     4261 ctgcaatttg tccaataggc attttcatat actttccagt tgctgtcact gctatatctc
     4321 catttggtag aattatttct cctgtacctt caaataattt tctgttttct ttagttattc
     4381 ttccaacaac ttttatagta gcatctgttg gtactggttt cttatatttc aactctaaag
     4441 atactgttac tccccatatt tggtcatcat ttatacttac agctcttcca atagtctcat
     4501 ccaatattgc agcagacata cctccatgaa ctctcccagg ataactttga tgccaatcct
     4561 ttgtattaca aattgataca agctctccat tttcaagttc ataaaaatct gctttaagtc
     4621 ctagctcatt ttgtgttcca caaactaaac aagatttact actattttgc ttcttcaaaa
     4681 ctttatactt tgccaaaaaa tccatctcct aaccgacatt agtagtcgct taatattaaa
     4741 taattctaaa atatattata taatattaat aaaatccctg caaacttcta ataataaaaa
     4801 taggctactt caatatgata agtttaattt aaagtattat tttccaaaaa ataactttat
     4861 caaaatagaa actcaaagta gatataagcc tctttttcgt tattaaaata gtaaaaatat
     4921 attttactta atataaaaaa gagtgtctca ttaactaata aagtttattt tgagacaccc
     4981 ctcataaaac aaattatact acaatagatt tttactaatt ctacaagtta tatatttaaa
     5041 ttatattatt caaattaaac tataattcta aatttcattt actattaatt cgcccattgc
     5101 tcttgttcct acaacatttc ccacaccatt atagatatca atagttctat aacctttatt
     5161 gagtacactt tgtacagcat cttcaataca cttagcttct tcttctaagt taaaactatg
     5221 tcttaacatc atgcttacag atagaatagt cgcaattgga tttacaatat cttttccagc
     5281 tatatcaggt gcacttccat gtattggttc atacatacca aaactatctt ctctaagtga
     5341 tgctgatggt aacataccta tagaacccgt aatcatagat gcttcatcag atattatatc
     5401 tccaaatata ttattagtca ctattacatc aaattggcta gggtctttta caacttgcat
     5461 agcagcatta tcaacataca tatgtgacag ttctacatct tcaaactctt tagctaagtc
     5521 ttccattata cttctccaaa gtcttgaact ctcaagcaca tttgccttat ctacacttat
     5581 aagtttttta tttctatttc tggctatttc aaaagctctc ttaccaattc ttcttatttc
     5641 attttcgtta tatatttcta catcataagc attttcgata ccatcaatga tttttcttcc
     5701 acgttctcca aaataaatgc cccctgttaa ttcccttaca acaacaaaat caatgccttt
     5761 ctcaactata tccgttctaa gtggtgaagc ttcttttata ctttcataca ttgtagctgg
     5821 tcttaaattt gcaaataaac ccaatgactc tcttaatttt aaaagaccag actctggtct
     5881 tttatctccc ttacatttat cccactttgg tcctccaaca gcaccaagca atacagcttc
     5941 gttttttcga caagtttcaa cagttttatt aggtagaggt tctccagttt catcaatcgc
     6001 gcatcctcca gctaatacat attcacaatc gaatctatga ttaaatttac aacaaatttt
     6061 atttagaacc tttatacctt catctataat ttccggtcca acaccatcac cttttatgac
     6121 tgctatatta caattcatac ttcactaccc cctatttgct tttattgagt taattaagcc
     6181 ttcatttaat ataatatttt gcataaactc aggaaatggt tctgctttat attcttttcc
     6241 ttttgttata ttttttatca cacctgtaga aaaatctact tctatattat ccccttcatc
     6301 tatgttattg gcagcttctt cacattcaag tataggaagc cctatattta tagagtttct
     6361 aaaaaatatt ctagcaaaag tagaagcaat cacacaggat actccacttt cttttattac
     6421 aattggtgca tgttctcttg atgaaccaca accaaagttt ttagttgcaa ctataatatc
     6481 tccttttttt accttagaaa taaatttatc atcaatatcc tccatacaat gcgttgctag
     6541 ttctttataa tcagctatat ttaaatatct agctggtatt attacatctg tatctatatt
     6601 atcaccgaat ttaaaaacac ttccattggc aatcataaat tttacctccc ttaaattatt
     6661 tctgatggtt ttgatatttt tccaattata gctgatgctg ctgctacagc tgggcttgca
     6721 agatacacct ctgaatcaac atgccccatt cttcctacga aattcctatt tgttgttgaa
     6781 atggattttt ctcctgctgc aagtataccc atatgtcccc ctagacatgg accacaagtt
     6841 ggtgtagaaa ctgctgctcc tgcctctata aaagtggtaa tatacccttt ttcgatagct
     6901 tcaagatata ctttttgagt tccagggaat atcataactc taactccatc tgctactttt
     6961 ttgcctttca ttatctcagc tactacttca aggtctgata ttcttccatt tgtacaagag
     7021 cctattacaa cttggtctat ctttatatcc ttatctatct catctactgt tctagtattt
     7081 tcaggaagat gaggaaaggc tactgtttct tttagtttgg ctaaatctat ttcgtatact
     7141 tcatcataga ctgcatcttc atctgcttca tatatattta catcttttgt catagttgaa
     7201 cacttatgtg aatttatata ttccatagtc ttatcgtcta ctggaaaaat tccatttttt
     7261 gctcctgctt ctattgccat attacaaata gtaaatctat catccataga taagtattct
     7321 actccatctc cacaaaattc cattgacttg taaagtgctc catcaacacc tatctcccct
     7381 attatatgaa gtattatatc tttaccactt atccatttat ttggtttatt cttcaaaaca
     7441 aattttattg ctccaggaac cttaaaccaa cattttcctg tagccatacc tgctcccata
     7501 tcagttgaac ctacaccagt agaaaatgct cctaatgctc catatgtgca agtatgagag
     7561 tcagcaccta taattacatc tccagcagtt actatacctt tttcaggaag aagaacatgc
     7621 tctattccca tatctcctac atcaaaaaaa tttacaactc cttttgattt tgcgaaactt
     7681 cttgtaaatt tacattgttc tgcactcttt atatctttat taggtgtaaa gtggtctaaa
     7741 accatcacta ctttagtctt atcataaaca tcttcaattc ctaatttttc aaattctcta
     7801 atggctactg gtgatgttat atcattacct aaaactacat ctaaatctgc ttcgattaat
     7861 tgccctttct taacttcatt taatttagca tgtttggcta atattttctg cgtcatagtc
     7921 atccccattt tttatttcct cctcaatata acttttattt attgcattca taagtgcatt
     7981 aatagctgct gtatttatgt tttccgatat acttacacca aaatagactt cattattgtg
     8041 ctctattcct acataagcta cccctcttga atgtgaccct tcctctaatg catgttccca
     8101 gtaatatcta aatttataat ctttcatgcc acattgcttt agtgcattat taaatgaatc
     8161 tacaggtcca tttcctatac caacaatcct ttgttcattt cccatataac ttattcttgc
     8221 agtcatctca atcatattag tatcattaga atcatcattt tcatagttta attcatccat
     8281 tgattttatt ttatacttct taactttata agggctctca acttcaatat attctttctt
     8341 aaacaaatta aatattgctt cactactaag ttcttcccca attctatcag attccatttt
     8401 gactacattt ccaaaatcag aatgcatatt tttaggtatc ataaatccat aatctgtttc
     8461 cattatataa acagctcctc cttttccaga ttggctattt atccttataa tttctttgta
     8521 ttctcttcca atatcatgag ggtctattgg taaatatgga acttcccagt aatcattatc
     8581 tctgtttttc attgctatca tgccttttct aatagcatct tgatgtgaac ctgaaaaagc
     8641 acaatgtaca agattacctg catatggatg tctatcatgt accataagtt ttgtacagtc
     8701 ttcatatata cctattatct tatctatatt gctaaaatct agctctgggt ctattccttg
     8761 tgtataaaga ttaagtccca catttactat atccatattc ccagttcttt caccatttcc
     8821 aaatagtgtt ccttcaagtc tatcggcacc agccaataat ccaagttcac ttgcagccgt
     8881 acaagttcct ctatcattgt gagtatgcaa acttagtata atagaatccc tacaacttat
     8941 atttttacaa aaccattcta tttggtctgc atatacgttt ggtgttgcca tttcaactgt
     9001 tgatggaaga tttattatta cttttttttg aggagttggt ttccaaacat ctatcactgc
     9061 ctcacatatt tcaagtgcat aatccatctc tgttcctgta aaactctctg gtgaatattc
     9121 aaaagtaaat tctgtttctg gatatttttt tacttcttca ttaaataatt ttgctccttc
     9181 aactgctata tctattattt cttgtttact catattaaaa accacatctc tttgaagaac
     9241 agatgtagag ttatataaat gtataattgc ttttttacat ccccttattg attcaaatgt
     9301 tttttctata agatgagccc tagattgtgt aagtacttgt atagttacat catctggaat
     9361 catattctca tctattaatt ttcttaaaaa tgtatactct gtatttgatg ctgatggaaa
     9421 tcctacctct atttctttaa atcctgtatc aataagcatt ttgaacattc tgactttttc
     9481 atttacactc ataggtgttg gcagtgactg atttccgtct cttaaatcga cactacacca
     9541 aattggagcc ttaaatattt cattatctgg ccatttacga tttactattt gcattttatc
     9601 atatttttta tactttccac acttcatgtt atttccccct ttttctgttt tttgtattca
     9661 gtatacctca tatcgacgca atcctgcaca attcataagg ataaatgtca attttatcat
     9721 tagaataaaa aaaacctcat ctctaataaa attagagacg aggttttgct cgcggtacca
     9781 ctctatttag ctttctaaaa agctccctta gtaatagcca atacaataat tataatgact
     9841 atatttccta taacgtggaa ttactccgat tattctaaga tttaaatctt agaaaacagc
     9901 tcctagacaa gttcagaact atcaactact aaatcacacc atcctttagc tctctgaaag
     9961 tttcattgtt cttactactt ctattcatca cttttttaat catttaaatt ttctaaatat
    10021 tttgatttaa tagttattat atacacatta aaatgtattg tcaacacaaa aaataattat
    10081 ctcaattttt tatttttgat aagcaattct ataaaacatg ctaactattg aaaacagtat
    10141 gatattatgc cttaaacttt ttcatctttt catctatgat attcaataat tttttttcat
    10201 cttccaaact caatgccctt aatctaattt tataattaga ttcgttagtg tatatattta
    10261 atatattttt tactacttct attttttcta ttatcttata attaaacttt tgactttgtt
    10321 tagattttat actgaatata cattgattat ttattaatat tgaatgatta tcgagagtaa
    10381 tatatacttt tccctcatat ttatttgaaa aatcacgacc ttgtttaaat agaaaaactg
    10441 ctgttattat acataaaata tataatattg tcgttgaata tataaaaatt gtagctaaaa
    10501 ttataagtgc taaaaccata tatatagtca ttcctttttt aaaataacca ttgtcataat
    10561 aattcatata gataaactta tttttactca aattttttca ctcccaagat tctaaaaatt
    10621 tatatcaaaa aattctataa tttcatcttc atgataattt atgactaatt catctggaaa
    10681 atctactgct tctaaaactt tttctgcatt ttcaaattct cctatagaat aacatatatg
    10741 agagtcagta ccaagaatta ctcttgcacc atatgtttta cacaatgtca acatatgact
    10801 tacattttcc caagtacccg ttctatgaga gtttgaactt aatgatgaat tatttatttc
    10861 taaaaggata tttttatctt tagctttttt tactataggt tcataatcta aaggatacct
    10921 actgtcatct ggatgtccaa ttatttttac cttaggttta tccataactt ttaaaatagc
    10981 atttgtattt tcttcttttg taccagaagc tatacaaggt ctatgcaaac ttgcgattat
    11041 ataatccaaa tgttgcaaca tatcttcttg aacatctaaa ttaccattat aatccattat
    11101 atttccttct attcctctaa gaatctttac gccttgcact tcacgtggaa gcaaatgcaa
    11161 attataaaaa tagaatggat gtggtcctcc tggcatatta ggtccatggt ctgatattcc
    11221 taagtatttt atattcttct tactcgcctc ttcgacattt tccttgaaag tactatgtgc
    11281 atgtccactt attagagtgt gacaatgtac atccataata gactttttca ttaaatttcc
    11341 tcctaatatt ttacataaat tcgtgaaaat agctaaaggg ctatttcaaa actaaagctg
    11401 tcataataga cagtgtaacg ttttaaaaca gccccatttt caattatttt acacttctaa
    11461 atctttaaat ttatctttaa ataattctcc aatacttcct aaagattcct ctacttctag
    11521 gtattctgtt atatcctctt ctggctctct agttgcttct tttattgaaa ccaacattct
    11581 cttagtcttt ggtttaaaat caagaatctt aactttaacc tcatctccaa tattaacaac
    11641 attagtaact ttagccactc tagtatcact caattcagat attggtaaat acgcatctac
    11701 accttcttta atttgaacaa aagccccttt ttctattatt ctaagaactt tagcattgac
    11761 aacatcatca atttgtactt cttttgctat taaatcccaa gggtcattat ttatgtcttt
    11821 taatgccaag gcaatttttt tagtctcttt atttacatcc aacacataaa cttctacttc
    11881 ttgtccctct gaaacaacat cttcaacact ttcaactctt gtccaagcta aattatttat
    11941 atgtgctaat ccttcaatcc caccaatatc cacaaatgca ccataaggca ttatctttgt
    12001 caccttacca tctctttttt gtccaacttc aagagaatta aataagtctt ctttagctgc
    12061 tttgatacga gctttttctt cttctctttc ttgcttaatt cttgctcttt cagcttcttt
    12121 ttctgcctta atttgagctc ttctttcttc tctctcttta tttatacgct cttgtaatac
    12181 atctctatga gatgctacaa gtctattttt tctaacatcc aattctttta tataaacttc
    12241 taagttaaga ccaacaaatt tagatgtatc tgtaataaat tttgtatcaa gttgagaaat
    12301 tggtataaaa aatgtatatg tattgtaatt agcaaataca cctttatcta tattcttttc
    12361 aacatttaca gttattattc tatgttcatc ataagccttt tgtaactcgg caaaatcatt
    12421 tcttttgtca agttgtaatt tagataaagt tattgtacca tctttttgag atacttttgt
    12481 tattacagca gttatttcat cacctatgtg gtatatatct tctatatatt gatttttttc
    12541 tatgttcaat tcacttatag gtattattcc atcaaaccca tagtctaatc ctaattgtac
    12601 ttcatcattt ctcactgcag ttatttttcc agttgtcaat tctccaactt tcactttgct
    12661 gaatacttgt tcttgctggt caagtaattc ttgcattgtt aaatcatttt ccattataaa
    12721 catcctttcc tcttattgat tatcatatct gtaaatatta taccattaat attgcaaata
    12781 cttacatttt tcttactata atttttaaaa taaattcaac ttcatatatt tatacattat
    12841 agaaaatatc ttattaaatt aaaatacaaa ttctaatttt tatctaataa aatattttca
    12901 taaaaaattt atattgtata tcttattctt ttattgcttt aggttttgat agaaaacatt
    12961 tataaaatat aaagaaagaa attgctgtta tagtagcagc tgttatatct gcaactggct
    13021 ctgctaaaat tactgcaaaa agtttttcat taaagaatgt tggaagtata aatattaaag
    13081 gtattaaaag tacaatcttt ctaagtagag ctaaaaccaa agatattgtt gcttttccaa
    13141 gtgctaaaaa tgtctgttga caagctattt gaatcccaaa cataaaagca ccagcaaaat
    13201 atatttttat actccaacta gtcatactaa gtaactgcgg attcttatta aatatactaa
    13261 caaaaaatgc aggaaatatc attaataaaa gccataaaat agtagaatat gtaaaacatg
    13321 acaacaaaca caacttaaat gtctttttta ctctatcaag ttgacctgaa ccataattat
    13381 aacttattat tggttgtgct ccttgtgtaa cccccatatt aggaagcata agaatttgca
    13441 ttatactgct cattatagcc attgaaccta ctgctaaatc tcctccatac atttgtaatt
    13501 tactatttaa ggctataagt actaaacttt ctgtagattg cattataaat ggagagattc
    13561 ccaaaccaag cactggtaag atgatacttg cttttggtat catatacttc ttttttattt
    13621 taagtatact tttctttcca aagagaaaca taagtaccca aatagcagaa acgccttgtg
    13681 caattatagt agctaatgca gctcctttta cacccatatt aaagccaaat ataaaaatcg
    13741 ggtcaagtat tatgtttatt aaagcaccta tagcaactgt catcattcca gttgtagcaa
    13801 acccttgggt atttataaaa gtattcatac ccattgaaat ctgaacaaac actgtaccta
    13861 ttaaataaat atttaaatag tccattgaat atccaattgt agattcactt gctccaaaca
    13921 tccaaagtaa aggctctttt ccaatcttaa aacctatagt caaaagtatt gctaaaatca
    13981 ccagtacaga aaaactgtta ctcattattt tctctgcctc atcattgttt tgctcaccca
    14041 tttttatagc agctaatgga cctcctccca taccgattaa ataactacat gcagttatta
    14101 taattattat tggaaatgcc actccaacac cagccatagc aacttctcca ttttccattc
    14161 taccaataaa aatcctatcg actatgttgt ataaaacatt tacaatttgt gcgataatag
    14221 caggtgtagc aagtttaaat aataatttac ccacactttc actacctaag ttagtgtttg
    14281 aattttccat tttaaaacct cctcaatcca ataattaaaa tgtatcacta aaattttatt
    14341 ctatataaaa atttcatttt attagcagac aaatttagag tactttccct acaagtaata
    14401 ctatactata tttcttttgc catagtaaac attttagaac taactgaagt taaatacttt
    14461 atctcatttt ttatttatta ttaaaaattt ttcacacata ctcataaaaa aactatccaa
    14521 gtatattact tagatagttt tatattatat gtaaagaaag agctgaaact ataacccctg
    14581 ataacaaatt gccaagaaat actcccataa aagcatcttt tattctcatt tttaatatgg
    14641 aagctatagc agatgcagtc cacgttcctg tagttggcag tggtatacct acaaatagta
    14701 ttataccaat tatcgaaaca ctttttaatt tcttcattct tgagtttatt tttctatcaa
    14761 ttacatcagc aatcccttta aatagtttct tccctcttaa aaattgtaat acatgtctga
    14821 atgtcaatat caagggaatc gataccaatg tagaacctat tacactaaat acataaaccc
    14881 atataggatt taagcccatt gcaataccta taggaatggc tcccctcaat tctgttacag
    14941 gaaccataga taaaattaat atgtgtaaat atcccaaact attccctcct attctaagtg
    15001 acattaagtt atgaatttat ttgctctata acctcaccat cctttttact aactaaaaat
    15061 gcatttttag tcaatgaaag cattggtaaa tcactcttag agtctgaata tgcatatgaa
    15121 ttttcataat caatctctat cccaagttta cctaatacag cctttattct ttttactttt
    15181 tcaattccct tacagttttc tccaatgaat ttatttttat atcttgaatt ttcataaaaa
    15241 aactccgtgc ctataacctc gtctgcaaga ccataatact taaaatattt catatatgca
    15301 tatggagatg cagttatcat aattataaca ttatcttctt ctttattttt gtaaataacc
    15361 tttttcatag attcgttaat ttttttcatt atgcaatcat caaaaaactt ttttaaatct
    15421 tcttcactaa aatctactat tggataaaag attgcctctt ttgccttctt caatggtatt
    15481 attctaatta aatacataaa tagaataaaa ataatgtatg gtaactttat aatagtttta
    15541 aaggttttat tttttaaaga gtaaataaaa aaatctatca tgctatcagt atttataaca
    15601 gtataatcta catcaagtaa taatacactt tttttcataa gtatttcccc atcctatcac
    15661 taaaaatatc actaaaaaat caatattatt aaacatgtaa ttatccataa gattacgttt
    15721 atttttaaag cattatcttt taagacaata tcagtaggat tattttcatt tgtagtgtta
    15781 attatataat tatatctaag tacaccataa actacaaata ttgttgtaaa tatcatatgt
    15841 ggcttttctg gattacttgt agaatacaat gcataacata caattgtaga acttaatata
    15901 actatcatca tttgatttag attttctatg tcataatctt ccaatatttt tctatgttca
    15961 actgcatcat ctcttagagt ttctatttct tttttacgtt ttccaagagc taagtacaga
    16021 gataagaaaa atgtacataa aattatccaa tttgataaac taacacttat ggctacacat
    16081 cctgcataaa ccctaagtat aaaaccaaga gatattgaaa acacatctaa tagtattata
    16141 ttctttagtt taagagaata taatatattg tttatcaaat agagactgag aattatagaa
    16201 agattaaaac taacaactgt tgataaacag actgaaatta aagctaatac tacaaatagt
    16261 atattagcac tttttttgct tattcttcca cttgccactg gtctattaca ttttttagga
    16321 tgttttttat ctttatccat gtctatagca tcattcagtg catatactga tgatgacatt
    16381 agacaaaaac acacaaacgc aataaacgta tttaaaaata aatttatatt tagtatgttg
    16441 tttgaaaaaa ttatagctga aaatacaaag aaattcttta tatattgctt tattctcatt
    16501 aactttatat aatctgaaat tttagtaaga agccttatac ttgtattggt ctgtttcata
    16561 aacttactac cctttcaata ttatttttta aacaggatat gattttatcc ctatgtttaa
    16621 aatttctata aatccattga cattactgat tactatagta accaataaca gcgatagaaa
    16681 tatattatac atattctttt cattttttct aagcgctttc gtaaagtaag ccaataaaat
    16741 tataactgca aaactaaaat ttatagccat aatacttgaa ttgactagat tatatcctac
    16801 tataccatgt aaagttagat tgaatataat tattcccata catagcttag gaactctttt
    16861 ttctgaattt tcaataactg ataaaacaca aaaacataaa gctaataatg taagaattac
    16921 tggtattgta gcaacttctc cattttgcaa aaataaccct tttttaatac ttgctccagg
    16981 aaaataaaaa accgaataca ataatctctc tataatcatt gcaatctttt ccaaaattgg
    17041 ttctccactt ataaatttcg tatcatactg tattacattt ccaaggttca taaaactctc
    17101 ataagacttt gttgaaacta cacaaatata tgccattagg ataaatgtaa ttaaatgttt
    17161 taacattttt cctattttta atttaaactc aaatatcata ttaaaaaaat acaaatatat
    17221 attactaata gttattccaa atagaggaat agctgctaat tctactaaat aagaattttt
    17281 tcctttcatc ttatttgcaa taactatgaa aaatattaat gaaaattggc tatatataaa
    17341 tctctctaca tttaaagtca taaataactg tggaaaagaa aatatcatta tcatagtaag
    17401 taaaattaaa gttaatctct gcatttttat ctttttaata tgattaaaga ctaaagttat
    17461 tgacatagca ctagttatta tttgtagaca aattagtcca aacccatagg cactattaaa
    17521 atctgttgtc ccaggatttg ctattgaagt acaaaatagg tatattggat atgcaaaaat
    17581 tgatataatt gctgaaaaca atatgtgctt actattgtct tgacttatag caaatacatt
    17641 taaattgaat aaaactcctg tatctgtatc taacaataca tcataagtat tgtttaaatc
    17701 ataaaatata gtagtcttag aaaaaaatat cttagttatt attaagtaag ttattacaac
    17761 taatataaac aaaaacatct cgtttttata ttttaatatt ttactcatca atatatcacc
    17821 atccattcat tatataggat ttgctgacac ctacaattta ttccttatat ttaatagcca
    17881 tcttcataat gttcttactt gttttattgt aattgaataa ctctttattt acaaccacaa
    17941 tatttacttt attgatgtta taatcagtca agtattgttt atataacata gattagtgtt
    18001 gtataaaatg agaatttgta agcaatatta atgtaaaata tttgaaaaac tttatataaa
    18061 aatatacaat gttgcttatg gaattaaact ataacaaatt aaagcataat ttaaggagac
    18121 aaaattataa tctgtctcct taaatattaa aactatattt taaaacatta ctaaatacta
    18181 tacaaatttc aaatatagta taaaattaat tagactttaa cactattagc tccatcttct
    18241 tcccattttt taatttcttt taatctatct attataggta aatgctgagt acattcaact
    18301 tcacattttc cacaagatat acattcttta gcttttgcct tactattcat tgtgtaattc
    18361 caatgtccta agccataaac taactttgtc atttcttcat cactcttttt aaacatctgc
    18421 ttttcattat aaaaaagcat ataggctggg gtattaatac cttttggaca tactttacaa
    18481 taaccacaac ctgtacatat ttcgttcata ttcttattta atctagtctc aatatcatgt
    18541 atatcctcat ctgaataaat tctattttcg tctgcaattt tacatgcatc atctatatct
    18601 tgttttttat taaaccctat caaagatata cttatttgtg aaattcctat gttgaatctt
    18661 aaagctgatt ctatagtact ttcgccatct tttgatagaa aagaaagttc tttattatac
    18721 tttggaatag ttccaccact taatgggttc attgccacaa caccatatcc attatcaaca
    18781 gcatatttgg caccttttag cctatatgga aagtttaata tatttattcc catagttaca
    18841 ccttcaaact tattttcact tagaatatct ataacctcat ctcctggttg atgagatgaa
    18901 aatactatat ggtctatcaa tccttcttct ttacatttca ataatccttc atattgacct
    18961 ccaggtctca ttgctagttc gtaatgttcc atctttctaa tattccaaac atgataaaaa
    19021 tttattttag atacgcctaa tcgttctatt gattttttta cagcctctat agctttttct
    19081 gatgtatcac atgcttttgg cttttcttta gttgatacat aaaaatcggt cttaccttcc
    19141 tttatcattt gtctaaaggc tagtccaaat atatcttcac ttctgtcatc acaataccct
    19201 ggtgctgtat caaagtaatt aatacctttt tcatacgcat atttgactaa atcagcattt
    19261 tcttgattac ttttttctaa atcaaacctt aatcctccat agccaactac tgaaaccatt
    19321 ttccctgtct ttccatattc aacgtatctc attgtaaatt cctccttaga tatattaaga
    19381 cttaatttct ttatagaaaa tattatacca taaataattt ttataaatta tttatggtat
    19441 aatatttttt ttataaatta tattttttta taacttgtat tttataacta agttaaaaac
    19501 tataaaaagg ttcaatatgg actattactt gagcattttc attaatttct tcttgtattt
    19561 gtttctcaat tttatgtgtt aaatcatgag attgctctac gcttataaat gggtctacca
    19621 ttacatgcat gtcaatataa atagaacttt tgctacctct acttctgata ttgtgaacat
    19681 cccttacctc attaaattca aacacaattt cttttatata atcctcatct acagccactc
    19741 tatcgaccaa aattccaata gatggcctaa atattccata agccgaataa attatgaatg
    19801 cagaaataat aataggaact atagattcta ttattactgg aaatcctaac ttaacaccaa
    19861 ctaaagtaac caatacacct agagacacaa atatatcact tcttgtatgc aatgaatccg
    19921 aaatcaaaac ataactattt agtttagtcc caattctata ttcatacatg catacaaaaa
    19981 tatttataaa aagtgtaata attaaagcaa ttaaactttc tatagttata gcaggtacaa
    20041 ctggattagc tattcttaac actgcactaa gaattatctt aattgctata acaaacaaca
    20101 taccacttat aaataaactg gttataattt caaacttttt atgtccataa ggatgatttt
    20161 tatcttttgg tttagatgca aagaaaatac ctataagccc cactatatta gatgctccat
    20221 ctgacaaaga atgaaatccg tctgctgtca tactatagct ttttatttga ttaccaataa
    20281 tgatttttaa taatgctact gcaaaattag caaataaaat tatccacaat acttgcttca
    20341 ctttcttata gttatccata acaaattccc cctcccttcg attcaataaa gaaagctatc
    20401 tatcaatatc aatcaaccag acaataaaat cgtatataag cacatttact atatttatat
    20461 tcaaaattgg ttgtacttgt tcattctatt tgaaacttaa ttataaataa aaatcgtagg
    20521 tatggttttg gtatacataa aaataagaga ctgtctcaaa ataaagataa tcttcttttt
    20581 tattgttgaa atgataaaat atatttgatt tcatataaaa aagagtgcct catgaactaa
    20641 aaagttcatt ttgagacact cccttaaatt tttgatataa actattaatt ttttatacta
    20701 agccttcttt aactaagaat gaatgagcaa cttcttctgg tgatttacca agttcatcca
    20761 cttgatagtt aagttctctc atagtttctt catttatttt tcctgaaagc atattcataa
    20821 ctttttctaa ctcaggatat ttcttcaaag tatcttctct tactaatggt gctgcataat
    20881 atggtacaaa gaaattttta tcgtcctcta atatcttaag attaaacttt ttaataagac
    20941 catcagttaa aaatgcatct ataacctgag attcattatt atctaaagct gtatatctta
    21001 aacttccatt cattgctttt acatctttga attttaaacc ataatctcta gaaagcccta
    21061 caagaccatc ttctctattt tcaaactcca aagttggtga gaatgtaaat tctttaccat
    21121 atttagtcaa atcagaaata gtatttattc catacttttc agcagtttca ggcatcatag
    21181 ctaatgtgta agtattatta aatccaagag ggtcaagtaa agttaactga ttttcttttt
    21241 ccatagtatc ttttacaaca ttatatgctt catcagcatc tttaatcgga gcatgtttca
    21301 ttacatttac caaaagtgta cctgtatagt caacatacat gtctaactca ccagatttaa
    21361 ttgcattaaa cgcaacagat gaaccaccta gatttaaatt cttcttaact ttaagattcg
    21421 tatttttttc tactaattca gcataaacat tgccaagtat aagttgttct gtataatctt
    21481 tagaaccaat agttattgta tcttttttac ttgaaaaact agaactaaaa acaacgaaca
    21541 acattgaaac aacaagcact acagatatta ctttaagtgc tatataattc ttcttaggtg
    21601 cttttggatt taatcctttt ggagtaactg caacttcaat tttactaaat aaataatcaa
    21661 ctaacagtgc aattatacat gatgggattg cccctgctaa aatcatatta ttatttacag
    21721 tttgaacccc tgaaaatact aaatatccca aaccaccagc acctataaat gctgcaagag
    21781 tcatcaaacc aacagctgta actgcagata ttctaactcc acccattatt ataggaagcg
    21841 ctaaaggaaa ttgtatttta aataatgtct gatttcttgt aagaccaata cctttagctg
    21901 attctaatac tactgggtca atacttgtta tacctatata cgtatttttt acaattggta
    21961 gtaatgaata aaccactacc ataaatatgg ctggtttact tcctatacca agtataggta
    22021 caagaagacc caaaagtgcc atagacggaa ctgcttggac taagtttaca aatccaagta
    22081 caaactttct aagagatgag attcttgaaa taattattcc aagaggaaca ccaactatta
    22141 tagctattaa aatagatgta acagttagac tcatatgttg tattaaaagc tctataatct
    22201 tatctttttg taatagtata aagttaaaaa aattagccaa gaacactcac ctccatttct
    22261 aagaattgtt cacttaaaac agaaagtaaa ctacttctag taataagccc taccagttta
    22321 ttttcatcag aaataaccgg aatatatcca accgaattac gattcattac atttagtatt
    22381 tctactaaat tatcaccttc atttacatgc aaaggctcac ttgacataat atcagcaagt
    22441 aatattgact tatcatcaat atctttcata tcttttacag taacaatacc ttttaatgtt
    22501 tttgctctat ctataattag taagctatca accttactcg ttctcataat ttctattcct
    22561 tgagttacag ttctagtaga attaacagca ataggatttt ttatcataat atctcttgct
    22621 tttatatatt ctggattatc ccaaactcta tcttctccga taaaatcttt tacaaagtca
    22681 tttgcaggct ttctaagtat attttcggga gtatcatatt gtgctattct tccatccttc
    22741 ataatacaaa ttttatcagc tatctttaaa gcctcatcca tatcatgagt tacaaatatt
    22801 atagtttttt tcaattcatc ttgaagagaa aataattgtt cttgtaaaga tgttctcgtt
    22861 attgggtcta aagcactaaa aggctcatcc atcaatatta tttcagcatc agtagcaatg
    22921 gctcttgcaa caccgattct ttgttgctgt ccaccactaa gttctgaagg atacttattc
    22981 aaaaattcat cactatcaag tcctaccatc tcaagtagtt gcaacgttct tttttcaatt
    23041 tcttcaacgg atttttcctt ttttaggcgt ggaattagct ctatattttc ttttattgta
    23101 agatgaggga ataggcctgt gttctgtata acatatccta tttttcttct aagctctatt
    23161 tcattttcct gagaaattgg tttgccatta atatatatct caccagatgt aggttttatt
    23221 aatttgttta taagcttcaa agtagtagtc tttccacaac cacttgaacc aatcaaaact
    23281 accaatgtac ctgtttctac aactagagaa atatcatcta atataacatt atttcctatt
    23341 ttttttgtta catttcttat ctctatcata atttatcatc ccctttttcg aacaagcaac
    23401 cttcttcaat attataagtt gtttttataa ttatatcata ctattttatt tatgtaaaca
    23461 aagtacgatt tttaggtcac tatttgtctg aatattgcta caaatataaa gtatttatat
    23521 atagtatata atacataatg tcaaaaaaca aaaaatataa aaatattcat aaaataacac
    23581 tagcctcaat tatatagtat taaagctagt gttatttcat ttaaacaaaa ctcacatttg
    23641 gatactatcc atgtattcaa ataatgtaat atctttataa gtttcttttt tatcacttag
    23701 gtttgatatt gtaactccca atagcctaat ttgttttcca ttaacatcaa catttctcaa
    23761 caaattatct acaccacttc ttatatcatt atattcgtca atatagtgtt ctaaactaag
    23821 gcttctagtt atctttgtaa agtcctcata tttaattttt aatgttaatg tttttccaaa
    23881 cttctcagag ttttttaatc tatgacatac ttcttcacaa agttcatcaa gaatgttgcg
    23941 tgtttcttct tcatcaatat ccaaattatg acttaatgta gtctcagccc caacagattt
    24001 tctaacccta tttggttcca ctggtctatc gtctattcct ctagcaaatt tatataattc
    24061 ataacctctt tttttgaata tattttcaag ttcaaataaa tttaaacacc ttaaatcata
    24121 tccagttttt atacctaaat ttttaagagt atttgaagta acttttccaa caccaaaaaa
    24181 tttattcaca ggtaatttat ctaaaaagtc ctgggcattt tcttcggtta ttacagttaa
    24241 cccatttggc tttcttaagt ctgatgcaat cttagcaaga aacttattgt atgacacacc
    24301 agctgaagaa gtaagaccaa cttctctaaa tatatctttt tttatttgct ttgctatctc
    24361 aattgatgaa tcaatatttt ttttattttt tgttacatct aagtaagctt catctaaaga
    24421 taatggttca actaaatccg tgtatctata aaatatatct cttatttgct ctgacacact
    24481 cttataaaca tcaaatcgag gtcttacaaa aactgcatat ggacacctat tgtaagctac
    24541 agctgatggc atagctgaat ggataccaaa ttttctagct tcataagaac acgttgcaac
    24601 cacacctcgg ctttgtggat ttccaccgac aataacaggt cttcctttta attttttatt
    24661 atctctttgt tctatagatg cataaaaagc gtccatatct atatgaatta tttttctata
    24721 atgtttcatt attatcacct tttaaagtat aaatagatta attttattta tattactttt
    24781 taattatacc aaacttatgt ttctttttgg tattttttca ttattttctt tatatattca
    24841 tattttccat aaataaaacc atctataatc cattaacttc aatataattt ataaaaaatc
    24901 cttaaaaaca cttgcaaaaa ctatataaaa gctttacaat aggtattcca aggaactaaa
    24961 tttaaaattc cttatggata acagtatata tatttaattt atgggatgtt ttaaaaacaa
    25021 aagactgtct tatgcaagac agcctactga ttttattata attgtaatgg actgctcaaa
    25081 ctattacttt aaaacagccc atattttttt atataatttt aactatagta aagattttgt
    25141 gaaggatatt ctgggtcaca agttacatct ataccaagct ttctaaatgt ttgttcatca
    25201 tctctattta atatagtagt agaatgagct tgacaccctt ttaataatgc taacttttcc
    25261 atagcaaatt gtgcagttgg attagttaca gcacaaatac taagtgcagt taacacctct
    25321 tggcaactaa gtgaaacatt tctactagct aaagtctttg tttttaagtt tatgataggt
    25381 tctaatatta caggtgatat taagtgcata tcatcagata tattggctaa atgtttaatg
    25441 gcatttaata ctactgctgc agttccattc attaaatcag aacccttacc agttaatatt
    25501 gtaccatctt ctaattctaa tgcaactaca ggacaagtat cattcttgtc agcactttct
    25561 ttcaatttat tactgtagtt tctagcaggt aatacaacat ttctatcttc tggctttaag
    25621 ttaagctctt ccataatcat tttcattcta tcataagcac ctttatcaac ttgtcccttt
    25681 ttatactcac aggcagtttt aaagtatctt cttattattt cttctataga agcttttctt
    25741 attacttcat catctattat tccaaagcca actctattaa cacccatatc agttggagat
    25801 tggaaaattg attcttttcc agttatcttt tctattattt ttttaagcac tggaaataat
    25861 tccaaatctc tattgtagtt tactgacatt tcaccatacg tttcaaggtg gaatgaatct
    25921 atcatattca catcttttaa atcaactgta gctgcttcat aagctatatt caaaggatgt
    25981 tttacaggta cattccaaac tgggaatgtc tcaaacttag aatatccaac tacattacct
    26041 cttttatttt catgatacaa ctgacttaga caagtaccaa gttttccact attaggtcct
    26101 ggtgctgtga ctactactat tggctttgta gtttctatat attcatttgc tccatatccc
    26161 tcatcactaa ctatagtctc aacatctgac ggatatccct ttgttggata atgcttataa
    26221 actttaattc ctctacgttc taatttattt ataaatacag ttgtagcagg ttgtccttca
    26281 aatctagtaa ttactacact atttacatct agttcataac ctcttaaatc atctattaat
    26341 cttaaaactt ccatatcata agtgatacca aagtctcctc ttattttgtt tctttctata
    26401 tctcctgcat agacacaaat tacaacttca actttttctt tcatatgttg taatactttt
    26461 atctttgcat tttcatcaaa tcctggaagt actctttttg catgaaggtc tcccatcaat
    26521 tttcctccaa actctaaata aagtttgtca aaattattta ctctttctaa gatatattta
    26581 gactgctctt ctaaatattt tttgtggtca aatcctactt tcatcttaac acctcactaa
    26641 attaaatttc ttattgataa cagtatattt atatatattt ttttagtaaa acatcttata
    26701 aaaaaatgtt aatacttttt cactcaaagt aatattatac aattaagatt aatttttttc
    26761 aagatagttt tatggtaaat aagatatacc taatataact aataaaataa gaatactata
    26821 tttttatata taagtataat attctcacct tagcattaat ttatttcaat attttcatat
    26881 atcttttatt ctaaattcaa tacttttcta atatcttcta taccttgata aacttcttgt
    26941 ttagaaaaat tcatagcttc taattcttca tcagtttttt ccataagcat agtataaaat
    27001 tctattgcta tatactgcat attttcacta tttacactct tagctttatc aaataaaatt
    27061 tcttctgctt cgtttacatt tccctgaaaa actaatcttt ttaaagttat aagtataact
    27121 tcttcttctg tattatacac taaatctttt tcttcatatt cactgtattt ttttcccaat
    27181 aaaagtcttg caaccataag ttttatctca ttgaaaatct tatccactat agccattttt
    27241 acatctccta taaataaatt ttattttctt tttaattata tcagacttta tcgtaaaaat
    27301 tattatatgt aaatgaaata attcaaaaat aaattttaac aataaaaaaa agaaggtagc
    27361 gggggatttc cgactacctt caaacactag attgtgattg gtttggtatt ttatttatac
    27421 ccaccataag ctttattaaa tcatctaaat tgattttttt agcatccaca tcctccatca
    27481 aaaacgtcac cacattcaca aagtattaat aataatatta agaaaggtat ccatgcacat
    27541 aagtccattt cagcccaaca atcttggaat gctaagaata agaagaataa tattattatc
    27601 caccaagcac atccaccaaa tccgccttca ccaaaaaatc tatccaacat aatatgttac
    27661 ctcctagtca ttttgtatgt ggagtttcag ctcctaaaat atactatgtt taaggggtaa
    27721 tatttgctaa tgattttttt aaattttttt agatattttt aaaaaaatta ttgattcaca
    27781 ctcaatgtct taccatcact attgataact acctctccat tagaagtcaa aaatgttttt
    27841 atattcaatc tttccaatat ttttaaaaca ccttcctctg gttctttttt ctctgaacat
    27901 gtaataacag catattgggg tgaaatagac tctaaaaatg tttcagttaa tttgtcatat
    27961 cttccatgat gaggcatttt aacaaaatca tattttaaag tatttccatt tataaactct
    28021 gacaatcttt cttcctctgc atctcctgca aataaaaact tatgtgctcc atagcttata
    28081 ttagtgatta ttgaataatc atttgaactc tcgtacttgc tttttgaagc cggatgtatg
    28141 tttatttctg cactattgat tacaatattc atattatctt ttagtagcac tggttctata
    28201 tcagcttttt taagtgaatc aatatattca tcatattgct tactatcttt cttataattt
    28261 gcttgtataa tattctttac tttaacacta gataacacac tatctactcc acctatatgg
    28321 tccttatcta aatgagttaa aattaaatag tcaagtgtat ttattccttt ttcttttaac
    28381 ctatccacta tttgttttcc atttttatct tctcctgtat caatcattat gactttgtta
    28441 cctatttcaa ccaatataca gtcagacttt ccagtactta atatagtagt cttaaatgca
    28501 gtattttttg tgctactctg aatattattt tgactcacat catttttgtc actttttaaa
    28561 taagattcat tcgttgattt tatcttacta ttagtagtta tatttgttac aagcaataat
    28621 attactatta tagataatgt agtaactaaa aacaggatat cttttaatac tattttcctc
    28681 atccaaaagc tcctataaat tttatttatt acatatttat agtatagcaa ccttaactct
    28741 gtaaaataca catataaaaa gggcaaattt taggcatatt tgtggcatga taaaaaaatg
    28801 actgtaccaa aacaattatc tttctaaatt tacatcttca aattctttaa tcttatcatt
    28861 agataaaaca ctctttttag tagattgtcc attttttcta tcaaaagtat ttttagcttt
    28921 tacacctgat aaaaacttac ctgctccatt aatacaacct tcttcacaca tcattccttc
    28981 tataaaatta ccttctaatc ttcccaattt agccattgtc attgtctttt taatttgtct
    29041 tggaccagat acttttatag gttcaaaatc aaaactaagc cccttttcag caatatagtt
    29101 ctgtactgat ttggcaagtc caccagatgc tccaaatccc cttccaaaaa tcgaaccatc
    29161 atttatatct tcatttgaac attttgataa atctatatta aatgcttcaa ataatgccat
    29221 tagttcttca aaagtaagta cataatctac tgaatctttt aaacttttct ctgttgcctc
    29281 actcttttta gctgtacatg gacctataaa tacaacttta gcatcagaat ctattttttt
    29341 tactaatctt cctgttgcta ccataggaga cactgtagat gatattctat ctttttcatc
    29401 tgggaacatc ttctctatat acttaaaaaa tcccggacaa catgaatttg tcatgtaatt
    29461 atctcctgat tttattcttt caataagttc attgctttca tgaactgtaa ctgcatctgc
    29521 tccacaagaa gcttctatca tatctacaaa tcccagtgat tttatagcat tttttaactg
    29581 accatatgta atattactat ctatttgacc tgttatagct ggagctacta ttgcatacat
    29641 attttctttc tttgcaagtt tttttgcaac aggtgctatc aaactcttat cagatattgc
    29701 tccaaatgga catgctgaca tacatgctcc acaatttata caattatctt catgtatcat
    29761 tgctttcata gtgtttctat taaaatccaa agctcctgtt ggacaaacgc ttttacaagg
    29821 tcttacaact tctgatatag catcatattg acatgaactt ttgcacattc cacaagcctt
    29881 acataaatca tgatttatat atgctctccc atttatataa gaaattgccc caaatttaca
    29941 tgcttcctta catctatgag ccaaacaccc tctacaaagc tctgtaactg taaattcatt
    30001 tattggacat ctatcacatg ctgcttcaac tatatatata atttgctcat ctgtgtccaa
    30061 actaactgaa tctggattta tatctccata tttactattt ggagttaaac ttaatgcaag
    30121 ttgcgccctt tcaagaacta cttctctctc tttataaaca caacatctat atctcgcctt
    30181 ttctccttta attatttcat atggaatttt ttctatattt tcttttgtta aattttcttc
    30241 aatagatagt cttgctactg ctgttaaaac ttcatgtttt aaccttttta gttgcgtttc
    30301 aaattcaaac ataagtaatc ctccctataa atcacacctt tttgctatat ttctactatt
    30361 atgtatattg tattttttat ttatttctat aaattattat aacataggca tatactttgt
    30421 taagttatat acaaaatatt atatatgtaa tttttatttt aagtgtatat aatttttatt
    30481 ttagttgttc aataattaaa tatataaata aaaatagagt tgtccaaaag ttaaacctca
    30541 acttagaaca actctattaa caatatgtat ttttgttgct ttgtattatc tttatttttc
    30601 taaatttata ttactaaaat cttttatctt tgtatttgct aagacacttt tcttagttga
    30661 ttgagaattt tttctatcaa aagtggcttt aacctttgca cctgataaga atttaccaga
    30721 accatttata caaccacctt cacacatcat tccttctata aaattacctt ctagctttcc
    30781 taaattagcc atagtcatag ttttcttgat ttctcttgaa ccagaaatct ttacaggttc
    30841 aaaattaaca ccaatacctt tttcagcaag ataattttct atagcttttg taagaccacc
    30901 agcagctcca aaacctcttc caaaaattga tgcatcatct atatcttcat ttgaacactt
    30961 agccaagtct atatcaaatg cctcaaataa tgccattatt tcttcaaaag taagtacata
    31021 atcaacagct ccttttaatt ctttcttagc tgcctcactc tttttggctg tacatggtcc
    31081 tatgaataca actttagcat ctggttcatg actttttata aatctaccac tagctatcat
    31141 aggagacact gtagaagata tttttcctac atgatttggg tatgtttttt ctatataacc
    31201 aacaaatcct ggacaacaag aatttgtcat aaaagcttct cccttttcca ttctttcagc
    31261 aaattctaag ctttcatgaa ctgtaactgc atctgctcca caagctgctt ctaatacatc
    31321 aacaaatcct agtgatttta tagcattttt tatttgacca tatgtaacat ttgcttcaac
    31381 ttgacctgtt atagctggag ctaccaccgc atacattttc tctttttgaa ctaactttct
    31441 agctacagga gctatcaaac tcttatcaga tattgctcca aatggacatg ctgacataca
    31501 tgctccacag tttaaacatt tatcctcatg tatcatagct ttcatatttt ctctatcaaa
    31561 tcctaaggca ttagttggac aaacactctt acatggtcta acaacctctg agatggcatc
    31621 atattgacaa gcttttttac acattccaca agccttacat aattcatgat ttatataggc
    31681 cattcctccg acatgtgtta tagctccaaa tttacaagct tctttacatc tatgtgctag
    31741 acatcctcta caaatatctg ttactgtaaa ttcatttatt ggacaactgt cacatgcagc
    31801 ttccaaaata tatataattt gttcatcttc ttctatatcc attgtatcta aatctatctt
    31861 atcttgacct ggttgtaacc ccatacttac atttactctc tcagatatta tagctctttc
    31921 tttataaaca cagcatctgt atgtaggttt ctcacctttt attatttcat actgaatatt
    31981 atccattgct tctctggtaa ggttatcttc ttttccaagt ctagctattg cagttaaaac
    32041 atcatgtctt aatttgtgta tttgtgtttc aaattgaaac attttaatac cacccccgta
    32101 aatgatttta tacgatacca ttataacaca atatgtcctt ttattaatat atacataaaa
    32161 tattatatgt ttaaatttta ttttatcgaa tatatatagt gtttttttgt tttattatat
    32221 aatattattg atatttctga aatttcacta tttttatttt tacttttcaa tctaatatat
    32281 aatatttttt attatatatt ttaattttac tttttactta catacctttt tgttacattt
    32341 tttgatatta aatttattat ctacatttac attttcgtca aattttacaa tacttctcac
    32401 tgtataattt aacaattttt attaaattct aaaagtaatt aattttttta aaaattttat
    32461 ttgcttttat ttaaaattta tgttatatta atagaacaga cttatttttt tagtttgtat
    32521 tttttaataa tgcggaggtt ttcttataat gaaaaacgga atagtaaaat ggtttaacaa
    32581 tgaaaaagga tttggattca tatctgtaga gggagaagat gatgttttcg tacatttctc
    32641 agctatacaa aatgatggat acaaaacttt agaagaaggc gaaaaggtta gctttgatat
    32701 aactcaaggt aatagaggac ctcaagctga aaacgtaaat agaatataat tatttaattt
    32761 aaatatatat attttagaaa aggagctatc gagggctctt ttttgttgtt tattatattt
    32821 atttttaatt acaaaaataa gaagttactt tagaaaagcg tcaaacttaa aataacttct
    32881 tatattataa tatatactat ttatataaaa tactctattt aattttatca gatattatta
    32941 agcataactc tcttaagatt ttacaagcta taactgtcga tacacctgtt gtatcgtaat
    33001 cagggcttaa ctctacaatg tcacaaccaa ctatatttat attagagttt tttataatct
    33061 taaaaatctc ttgaaactct ctataattta caccaccagg ttctggtgta cctgttcctg
    33121 gaaacacaga tgcatccaat acatctaaat ctatagttag ataaatattc tttccattta
    33181 gcatattaac tatattttca aacgtatcta ttcctcctat ttccatgtat gtgtgttttt
    33241 cttctgtagc aaatttaaat tcttcttttg tcccagacct tataccaaat tgaaatattt
    33301 tattatcacc tacaatatcc catattcttt taattactgt tgcatgagaa tttttactat
    33361 tattatattc ttccctcaaa tcagtatggg catcaaaatg aattacatat atatcattat
    33421 acttttcatg tactgcttta aaggctggta atgtaactaa atgctctcct ccaatcataa
    33481 agggtacctt tgaatctcta acaatcttat atgtctcttg atagatttct tttaagactt
    33541 gttctgtact tccaacacta atttctaaat ctccataatc acatatatta taatcctcta
    33601 aatccaaatc taaaaaagga ctatatgtct caagaccgta aaattctttt ctcatagagc
    33661 tacttgcaaa tctagcccca ggtctgttag aagtagttcc atcaaatcca acaccaaata
    33721 ctataagatt agattcttca taatttttgt tcatactcat aaaagtgttc atatgataga
    33781 aattattctt catccagcat ctccttcacg tattgaggta acataaacga acctaaatgt
    33841 atatcactat tataatattt agtttttaaa cttaatttct cccatccatt tctatcttga
    33901 ttttttactg gattaagttt ttttgaagca aatccaaaca accagtgacc agatggatat
    33961 gttggtatat gtgcttgata aacttctgat attggaaata tctgttttaa ctttttatta
    34021 gctcttttca tctctttagc attaaagtca aaatacggac tttcactttg atttactaaa
    34081 attccatcat cagtcagtat tctataacaa tccttataaa aatcagttga gaaaagccct
    34141 tcacctggtc ctattgggtc agttgaatct actattatca ggtcatatga tttatcatgt
    34201 gcacacttaa caaaattaac accatcttca aaatataaac tcactctttt atcatctaat
    34261 ttacatgagc atatatccat atactcttta gatacatcta caaccatttt atctatttca
    34321 accatgtcaa tcttttctat ttgaggataa cggcttaatt ctcttactgt acctccatca
    34381 cctccaccta tgactaatac tttctttatg tttaaatttg tagccatagg aacatgagtt
    34441 atcatctcat ggtatataaa ttcatcttta tatgttacca tcattaatcc atctattgtt
    34501 agaaattttc caaattcatc actatcaaac acatctatcc tttgaaattg agactttcct
    34561 tcaaatagat gtttatttac ttttatagaa aatcttacat tttctgtcca ttcttctgta
    34621 taccaaagtt ccatataata atcctcactt tctcttgtat gttttatact gcttctggtt
    34681 tatgagtaat cttacctaaa tctttttttg aatatttttt aatttttgta gttaagcctc
    34741 ttggaatttc tgtagactcg cttctttcag actttaaaag ttgctctaat aattcaaaac
    34801 ttttccatgg gtctatatca ccacaagtaa aaaaatctgc tgctgcataa ccatattcag
    34861 gccatgtatg tattgtcaaa tgagattcag caattataac agctcctgaa accccccaag
    34921 gattaaaatg gtggaataca gaatccacaa ttgtcgcttt tgcatttaat gctgaatcat
    34981 tcataaaatt ttctataaga cgtgggtctt taagtacgtt ttcatcacaa ttatagtatt
    35041 ctactagtat atgtcttcct agtgtttcaa atttcatttc aaccccaact cctttatact
    35101 tttaataagt tctatttgat aaactctact taacaaacac ttgcccttgc aaaataaagt
    35161 aatttatttt attactagta ttctatctaa gtttttatct tgtacatctg taagatatgc
    35221 attgctattt tttaagaaaa taatatactc cataatttct tctgttatca attctcctgg
    35281 agcaataatc ggtatacctg gtggatatgc cattattgat tctccacaaa ttctatctat
    35341 acatgaattg atttctacac tttcctttgg tgcataaaaa gcttctcttg gattaagttt
    35401 aataattgga tttatttgaa ccatattaaa ttcatttaat gttgattctt ttctataaat
    35461 ctttgcaatt atagaaagtg cctttactaa tctatctata tctgatttat ttgttccaat
    35521 ggacacaagt gccagtatat tatatgaatc tcccaattct acttgaattg aaaaattttt
    35581 atataataaa tcgtatactt caaatccagt caaatttaat tcttttacat ttatacacaa
    35641 ttttgtttca tctataaatt taacaccttt ttgtttcaat atttctgtag ataaaacttt
    35701 tattccatca attttgttca atttagattt tgcataccta gatagattta atgcttttga
    35761 caattgcttt tcaccattct caactagatt aactctagca ccatctatac ttgccataag
    35821 caaataagat gctgatgttg attgtagcat attgattgat tgaagaacct tttcaaaact
    35881 tacattatct ctattcaata agagtgctga agattgtgtc aatgctcctc cagttttatg
    35941 tatacttaca gctaccatgt cagctcccaa acttattgca gaaggaggta aatctaaatg
    36001 aaatggaaaa tgtgcaccat gagcttcatc aactaacact aaaacattat ttttatggca
    36061 tagttctata atactctcaa ggtctgcaca aactccatag taagttggat taaggaaaaa
    36121 taatgctttt atattacaat ctttttctat ctctgttcta actttttcaa gagtaatatt
    36181 taaacttata cctagttttt catcaaattc tggttgtata aatattggta ttcctccaca
    36241 aagtattaac gcatttatta ctgatttatg tatgtttctt ggaagtaata ctttttcacc
    36301 tggttttata acactcaaaa tcattgcatg tattgcttga gttgtgccat tcgttataaa
    36361 gaatgcctca tcagccatat atgcttgtgc taacaaatct tgtgcatttt ttataatccc
    36421 atttggcgca gatacattat ccatagttgg tgatgaattt atatccattt tcatgagact
    36481 ttcacctaga tatttattta atatagctac acctctattt tttacatgtc caggcacatc
    36541 aaaacaagct atatctttct ctgagtattc ctttaacgac tctaataatg gagcacactc
    36601 atagcttaat tcaactgcat ttgcaaactc gctatccatg atttcaaatt catctcctct
    36661 ctatattcaa atatttttaa tatttattat ttattatttc tattttttat ttttactttt
    36721 attaaactca tgatatttat tatattaaca tcccagcatt tttacaagtg tttttttgaa
    36781 gttactcaaa aacatataca ttagctatat caatatttat aaacttttta attttttgtt
    36841 gtttatatta atttttttaa aacttagtat ttataaactt tcttattttt tattattttg
    36901 gtttttttac ttttttgagt tttagtaagc ttttgagttt actattttta atttataagt
    36961 attttattac taaactttta tattattctt atttcacata tcaaaattct tggtttagtt
    37021 ggactaaata attttcctat ttttgtattt taattttaat ataaaattta ttaaattttt
    37081 taattgataa aataaattct atatttatga tattaaaatt ctataagttc attgatttat
    37141 atatgttaat ttaaatacat tattctatcg tagtgcctta gagtttttat ttgtatttaa
    37201 agttaaattt attcttgttt ttcctttaat atttaatagt aaaacaacaa aattataaaa
    37261 aaaataaaga gaaactttat aaaagcttct cttttttcat ctctgctata ggtatcgttt
    37321 tccaatgttc tctcaaaatt ctttcaacat tttctaaatc attctccctc atagatttta
    37381 ctacttcaat atgttgatta ttgctgtatg tcaacatttt atataactca tcttcattat
    37441 cactagtata tgactttgac ataaaattat atcttaaaga gttcaataga tttactaaca
    37501 cctgattatc tgatttagtt atgtatatat cgtgaaattc attacttaat tttaaatagc
    37561 tattgtaatt tttatacttt atagagaccg ccatcatttc tgttaattct tccattttta
    37621 ctaggtctga ttcagttaaa ttttgtaatg ctgaagaacc ggctaacgca tccaatactc
    37681 caattacgga ataggtgtct aatttctcct tttcctgaaa ttcttttact acaaatccct
    37741 tattaggtat tttttctagt aaattttcac ctgctaacct agttaatgct tctctcgctg
    37801 gtgttctact aactccgata gatttacaaa tctcagattc agtaattctc tgattagctt
    37861 catattctcc attttggatt ttctttgata tatagtcata tacttgagtt gtaagtggat
    37921 ctaccttcat aagacgttcc tccatattaa gattataaaa gttaaatctc cctacaactt
    37981 tggtagagag ttcagtttta tcttactaat ttatataata tcacaattat gatatttttt
    38041 atacttcttt gtaatatttt gaaatactaa aattcttatg ataatttata caaaaaaagt
    38101 agaaaaagtt ttgataaaac aaaactcttt ccacattcgc cttattttct atttattttt
    38161 ctaaaaaaag atatttaatt tactctaaaa attctaaatg atttagagtc taaatctact
    38221 ttcaactcat tattttcaat ttgtatagta ctatttgcat ttttaagact atactttata
    38281 ggtatttcct caataaaact actctctata cataatgata tactttccac attttcactt
    38341 ctatttacta aaattaacat tctatcatta ttttcattaa ctctaataaa agaaaataca
    38401 gaatcattat tagtatatat aaattctgta tttccagaac ttaaaagttt attcttattt
    38461 ctaattttta taacatctct ataaaaatta agcatatctt cgtcttcatt tttccatgga
    38521 taagttcttc tattatctgg atcaacacct ccacaaagac ctgcttcatc tccataatat
    38581 atatatggta ctccttcaaa tgtcatctgt atagctacag caagcttcac taaatctaca
    38641 tcatcattta attccgtttt agctcttgta acatcatgtg ttccaattaa atttaaatta
    38701 gacctaaatg attcttttgg ataattttct ttaatctgca tatatctatt acaaagttca
    38761 tatgaattaa tttctccttt taagaaagaa aacatgttat ttctaaaagg ataacccata
    38821 acagaatcta attcttcacc taataaataa cttcttctct gaccatagct aatcttattt
    38881 gatgcatctt cccatacttc acctataaga attgagtcat catctgcctt ttttaattcc
    38941 ttttttaact ctcttataaa cttagttggt aattcgtctg caacatctag tctccagccc
    39001 ttaataccca tatttaccca tttatttata acgctatcat tgtcatatat tatataatcc
    39061 atatagctat gttctaattc atttatattt ggcaatgtct taacatccca ccaactttta
    39121 tatttttgag gaaattcttc aaacatatac catgaataat atggagattc tttagattga
    39181 tatgctccta aactattata gtttccatac atattaaaat atttactatc tgctccagta
    39241 tgactaaaaa ctccatctaa tactattgat ataccttttt cttttgcttt atcaataagt
    39301 tctttaaatg tatcttcatc tccaaacatt gggtcaattt ttttatagtc acctgtatca
    39361 tacttatgat tactagaggc ttcaaatata gggcttagat atagtatact tacacctaat
    39421 ttttttaaat atccaagttt attgattatc cctcttaaat ttccaccatg gaaatcccat
    39481 cttataacat ccccttgact atcctttatg tacataggtg tatcttccca atttccatat
    39541 atgaaactat tttttttagg attatcaacc ttaccactac gatttccatt attgaatcta
    39601 tctacaaata tatgataaag tattccttct ttgtaccatg ttggtacttt aaaatcttta
    39661 taaacagtga tttgatattt attaatatca ctataattat attcacaaga atatccatta
    39721 tctctgtttt ttccataaaa taaagtcttt atttgaccat ccatttcaac atcaacttta
    39781 aagtaataaa aataaacacc tgtaccatca aacttttcaa tcttaccaca aaagcacttt
    39841 ccaagtttat cattatctaa ttcttcattt aaagttattg ttctcatttc ttcttctcta
    39901 tttatttcta gggaaataga cttgatattg tatccctcat ttactttaac acaaatggtc
    39961 aattcctcat caaattttaa agctccaaac ggagccttaa aatttttatc ccaagagtta
    40021 tattctatta cactttgcat ttaatcacac ccttttaaac caaatgtctt ttgcatactc
    40081 agaaattgtt ctatcagaag aaaaaaatcc agagttagct atattaacta aagacatttt
    40141 attccatttt tctttatctc tatataacct gtttatatct gcttgagctt gaccataatt
    40201 ttcaaaatct ctcaatacaa aatactcatc attgtaagta gtgagtgaat tataaatact
    40261 tcttccttct tcgccaagat taggaataaa tccatttatt aaatcatcta ctaccctttt
    40321 tatatccata ttagaatgat ataggtcaag tgaagaatat ccaccatatt tattataatt
    40381 tagtacttgt tctgcactaa gtccaaataa aaacatattt tctttaccaa cctgttcaca
    40441 tatctctaca tttgcaccgt caagtgtagc caaagttata gcaccattca tcataaactt
    40501 catattactt gttccagatg cttccttagt tgtagttgaa atttgctcac taacatttgc
    40561 tgctggtatt attatttctg ctaatgatac tccataattc tctaaaaata ctacttttaa
    40621 cttgtccttt actctaacat cattatttat agtacttgca acagaattta taaatttaat
    40681 tatacattta gccaaataat atcctggtgc tgcttttgcg ccaaatataa aagttcttgg
    40741 gtcaagattc aaattaggat tatcaagtaa ttcatgatac atatgaagta cattaaatat
    40801 attcagtaac tgtcttttat acgcatgcaa tctcttaact tgaacatcaa atatcgaact
    40861 tgggtctaca tttaaatcgt atttatcttt tataaacttt gctagatttg ctttattatt
    40921 gtattttata tcatcaagtc tttgtaaaac agaggaatca tttttaaatt tttcaatatt
    40981 ttttaattgt aatgtatctt tcttccatga atcaccaatt aaatctgtaa ttaaattgct
    41041 aagttgagga tttgaactta taagccatcg cctatgtgca attccattag tcttattatt
    41101 aaatttattt ggttcatctt gatagaaatc ttttaatacc tctgtttcaa gaatatgagt
    41161 atgaagctta gcaactccat ttacactatg acttgtgaca atacataagt ttgccatatt
    41221 gatattgtca cagtctatta tactcattct ttttattcta tcttggtcat atcctttatt
    41281 atttaattcc tctacatatc ttctatttat ttcttctatt atcatataaa ttcttggtaa
    41341 tagctctttc atcatactta caggccattt ttccattgct tctgacatta tagtatgatt
    41401 agtataggac actgtttttt tagttatttc ccatgcttca tcccaagata aattttcttc
    41461 atcaagcaaa attctcataa gttctggtat gcataaggta ggatgtgtat catttatatg
    41521 aattgctacc ttatcgaata aattattaat atttaatttg ttctttttat attttcttat
    41581 tatatcttgt atccctgcac ttacaaaaaa gtattcttgt ttcaacctca acaacttacc
    41641 tgcatagttt gaatcatcag gatataatac ctgtgatatt tcctcagtat aatacttatg
    41701 ttttaaagcc tcttcatagc taccagaata atttagagca tttgaggtaa gttcaccaaa
    41761 atctctcttt ggtatttcac tcttaaacaa tcttaaagta tttatacatt ggttttggta
    41821 tccgattatt ggtatatcat aaggcattgc cattacagga aggtagtttt tatgaattac
    41881 ttttaaatga ctaccttcct tgattaattc tacctcacca ccaaacttca ccattgttgc
    41941 ttcatttggt cttacagttt cccatgcata tcgtccttct gtaagccagt tatcaggcac
    42001 ttctacctga taaccattta caaatttttg ttcaaacaga ccatatttat accttattcc
    42061 atatccttga ccacttatgt tcaaagatgc catagaatct aaaaagcaag ctgctaatct
    42121 accaagtcca ccatttccta aggctgggtc tacttcagtt tcaattaaat catccaagtt
    42181 gattcctaat tcagataaac cttctctaac ttcttcttca atatttaaat ttaataaatt
    42241 agattttaac tgtctcccta ttaaaaattc taaactaaaa taataaactt cttttttctt
    42301 atctaacttt gtagcaaccc atttttttgc tatctcatct ttcaatagac tacataatac
    42361 atttaacaat tgttcatcag tagcttcctt gatagattgt gcatataagc tatacatttt
    42421 tacttcaaat ttttgcttga atttcttttt gcttattgtt accaaaaaaa ccactccttt
    42481 aatcacatat gtctctatat gtttctatat actccttagc tgattttttc caactattat
    42541 ctgttttcat tgcattttca actaatttaa tccatttttc tttgtcctta aatactttga
    42601 ttgcattttt caaacagaaa aacatctcat gagcattgta atttttaaaa ctaaatccat
    42661 tgccttctcc agtaaattta ttataaggtg ttactgtatc tctaagccct ccagtctctc
    42721 taactattgg taaagaacca tatctcatag ctaacatttg acctatacca caaggctcaa
    42781 ataatgatgg cattaagaac atatcacttg cagcatatat ctgttgagcc aatgatgcat
    42841 taaaagtaat tctagctgat actttatctg aaaaattaga atcgtaatag ttgaacattg
    42901 attgatattg atgttctcca gttcctaaaa cgacaatctg taaatcttct ctcataagtt
    42961 ctggcatcat gtatgctatt aaatcaaatc ctttttgaga aactaatctc gaaactatac
    43021 ctatcaacgg aatagatgaa tcttttttaa gtcccaatat atcttgtaat ctcaatttat
    43081 tttctacttt tttgtcaatt gagtctacat cataatgaac aaatatatct ttatcttttg
    43141 cagggtcatt taaatcataa tctataccat ttaatatacc ttttaatttt cctgactctt
    43201 ttctaagtaa tccatctaat tgttcaccat aaaaagatgt ttgtatttca tttgcataag
    43261 taggactaac agttataatt ttatctgcaa aatttatacc agctttcata aaatttattg
    43321 agccataata ctctatatct ccattatcaa agtatttttg tggtaagtcc aatatatcat
    43381 ataaaacatg cttatcaaat acaccttgat attggagatt atgtatagta tacatagttt
    43441 ttatattttt atacccctca agggttgcat atctttcttt tagaattaat ggtaacatcc
    43501 ctgtatgcca atcatttata tgtattacat ctggataaaa atcaattcta cttatagctt
    43561 ctaaaactgc atctgtaaag aaagtatatc tttctgcatc atctccatat ccatagatac
    43621 tctttctttc atcttctctt ctaaaataaa attcattatc tataaagtaa aattttacac
    43681 catctaattc catttctaaa agaccacagt attgatttct ccatccaaca cgtacactaa
    43741 acacagctat ttcttttagt tgatctttta aataacttgg tatagttgaa tatttgggca
    43801 ttattaccct tacatctaca ccttcttttt ttaattcttt aggcaatgca taagaaacat
    43861 ctcctaatcc tccagttttt gcaaagggcc aacactctgc tgttacataa aaaaccttca
    43921 ttagtcattc ccccttctaa aaaatttatt tatatatttg tattcttttc cactacaaga
    43981 ggatattttt catctcctct taattctttc ccatttgata cactcgtatt tttatctaat
    44041 attgcattat atagttttgc atttgacttt attacacaat tttgcattac tattgagttc
    44101 tctataactg ttcctttttc aacatttacc tttctaaata tgatactatc ttttacagta
    44161 ccttctatta aacaaccaga tgctataaag ctattttcta ctttagcact atctgtataa
    44221 attgtaggtg attcattctt ttcttttgta aatatctttc tttcagaata taataattca
    44281 tctgcaactt ctaaatttaa taaatctttg ctcatttcaa aatatgattc aacagaattt
    44341 acacatttta gatatcctgt atattcatag gctcctattt tcatattttc tgcttcttca
    44401 ataataaatt cttctaaata acaataattg cctttattta caatgttata tatacattca
    44461 acaaaatctt cacgcttcat aatatatgtc tccatacata tgtttgctct agggaactta
    44521 cctaaattat ttcctacatt tattacccta ttgttactat ctaaatttag agttaagtta
    44581 ccagcaaaat ctttatttgc attatttaca tttttatata ttatagttat atcattatga
    44641 gattttttat ggtattccaa tgcttgagaa taatttaaat tgcatagcat atagcttgga
    44701 gctattaaaa tatattcttc ttcactcttt tctatataat ctagattagc taagatagta
    44761 tatatatcac cttctctatg tgagtaagta tttctatact tagtgttttc tggactaaaa
    44821 attgataggc cacctttttt tctacttaaa tcccaatctt taccactacc tatatggtct
    44881 gttaatgatc tgtatttttc ttttgcgaat attccaactt tagtgattcc tgaatttatc
    44941 atattagata aagcaaagtc tattattcta tatcttccag ctattggtgt agatgctatt
    45001 ggtcttccat agttaagttt gtttattgct gggtctcctt ttttgtttaa atttattatc
    45061 cctaaacatt catttctcat cttaaagacc tccttctatt aatacgcatc taggttcgat
    45121 attttcatac tcagaaacta catttatttc attatttaca ttctttatct gaacattatc
    45181 atttatagtt gcacactcac ctatcatagc tttttctata gttacatttt cacctattac
    45241 aacatttggc attattactg aatcaattac tttacttcct ttaccaacaa caactccatg
    45301 tgataataca gagttaccta cttcacctaa aattctacaa ccatcagcta tcattgatct
    45361 atgaacattt gcatttttac ctatatattg tggaggcatt gccatagtat ttgtatatat
    45421 cttccactta gggtctgcta aatcaagtgt ttctggagat tttattatat ccatattagc
    45481 atcccataga ctttgtatag ttcctacatc tctccagtat cctttaaatg gataagcata
    45541 cattccaaca ttatcttcta acattttagg tattaaattt tttccaaaat catcataatt
    45601 tatttctgga tttttttctg cttccttaaa ataatttctc aacattttcc aatcaaatat
    45661 gtatactccc atagatgcca aattgctctt aggttcactt ggtttttctt caaattcata
    45721 gatacttgaa tcttcatttg tattcattat cccaaatctt gaagcttcat cccaaggaac
    45781 ttcaatcact gctattgtag ctttagagcc tttttcctta tgaaatttta acattttgct
    45841 ataatccatc ttgtatatat ggtcaccaga taatatcaat acatattctg gattataggt
    45901 atctacaaag tccatatttt gatatatcgc atgtgctgta ccattatacc aatttccttc
    45961 tttttcattc ataaatggtt gtaaaacata gacaccacca tttattctgt ccaaatccca
    46021 atgacttccc atgcctatat gtgaatttaa tatcaacggt ctatattgag ttaaaacacc
    46081 tacagtatca ataccagagt ttgaacaatt gcttaacaca aaatctataa ttctatactt
    46141 acctccaaat gatacagctg gtttggcaat tctttttgta aaaacaccta atcttgaacc
    46201 ttgccctcct gccaaaatca tagctaacat ctcttttttc atttaatgac actcccctca
    46261 tctagtaatt aatatttata attgcatttt tacttttata aactttatca gtcaataaat
    46321 gtcgtgtata aatgttgctt aaataatacc ttaataaaaa taaaataata tcaactatac
    46381 aattgatata atgtataccc cataatggac ttaccgacgt atacattatt ttccataagc
    46441 actatagtat ttattaatat tcaattattg gttaaaaaat aatacaaatt ttattttata
    46501 aatataaaaa cattttttat aaatcctttg tgtcatatga acatgcttat tttttaattt
    46561 gttaaattcg taatagcaaa at
//
LOCUS       DAEMGG010000016        44500 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEMGG010000016 DAEMGG010000000
VERSION     DAEMGG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.016"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..569)
                     /locus_tag="KPK24_000904"
     CDS             complement(<1..569)
                     /locus_tag="KPK24_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2298611.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIG"
     gene            739..2097
                     /locus_tag="KPK24_000905"
     CDS             739..2097
                     /locus_tag="KPK24_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298612.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            2090..2722
                     /locus_tag="KPK24_000906"
     CDS             2090..2722
                     /locus_tag="KPK24_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG2298613.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            3051..4208
                     /locus_tag="KPK24_000907"
     CDS             3051..4208
                     /locus_tag="KPK24_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2298614.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTLDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFILIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(4355..4990)
                     /gene="lexA"
                     /locus_tag="KPK24_000908"
     CDS             complement(4355..4990)
                     /gene="lexA"
                     /locus_tag="KPK24_000908"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG2298615.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(5094..6221)
                     /locus_tag="KPK24_000909"
     CDS             complement(5094..6221)
                     /locus_tag="KPK24_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2298616.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(6542..7387)
                     /locus_tag="KPK24_000910"
     CDS             complement(6542..7387)
                     /locus_tag="KPK24_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298617.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(7417..7788)
                     /locus_tag="KPK24_000911"
     CDS             complement(7417..7788)
                     /locus_tag="KPK24_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG2298618.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(7941..8201)
                     /locus_tag="KPK24_000912"
     CDS             complement(7941..8201)
                     /locus_tag="KPK24_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG2298619.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(8390..9334)
                     /locus_tag="KPK24_000913"
     CDS             complement(8390..9334)
                     /locus_tag="KPK24_000913"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG2298620.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(9355..10209)
                     /locus_tag="KPK24_000914"
     CDS             complement(9355..10209)
                     /locus_tag="KPK24_000914"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG2298621.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKLLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(10224..11585)
                     /gene="accC"
                     /locus_tag="KPK24_000915"
     CDS             complement(10224..11585)
                     /gene="accC"
                     /locus_tag="KPK24_000915"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG2298622.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(11601..12053)
                     /gene="accB"
                     /locus_tag="KPK24_000916"
     CDS             complement(11601..12053)
                     /gene="accB"
                     /locus_tag="KPK24_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG2298623.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(12323..12769)
                     /locus_tag="KPK24_000917"
     CDS             complement(12323..12769)
                     /locus_tag="KPK24_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298624.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            13044..13322
                     /locus_tag="KPK24_000918"
     CDS             13044..13322
                     /locus_tag="KPK24_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298625.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(13451..14197)
                     /locus_tag="KPK24_000919"
     CDS             complement(13451..14197)
                     /locus_tag="KPK24_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298626.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEI
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(14326..14970)
                     /gene="trmB"
                     /locus_tag="KPK24_000920"
     CDS             complement(14326..14970)
                     /gene="trmB"
                     /locus_tag="KPK24_000920"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG2298627.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(15108..15428)
                     /locus_tag="KPK24_000921"
     CDS             complement(15108..15428)
                     /locus_tag="KPK24_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG2298628.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            complement(15881..16129)
                     /locus_tag="KPK24_000922"
     CDS             complement(15881..16129)
                     /locus_tag="KPK24_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2298629.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(16160..16621)
                     /locus_tag="KPK24_000923"
     CDS             complement(16160..16621)
                     /locus_tag="KPK24_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG2298630.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(16659..17492)
                     /locus_tag="KPK24_000924"
     CDS             complement(16659..17492)
                     /locus_tag="KPK24_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298631.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(17766..18452)
                     /locus_tag="KPK24_000925"
     CDS             complement(17766..18452)
                     /locus_tag="KPK24_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298632.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(18467..20944)
                     /locus_tag="KPK24_000926"
     CDS             complement(18467..20944)
                     /locus_tag="KPK24_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2298633.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(21163..22566)
                     /locus_tag="KPK24_000927"
     CDS             complement(21163..22566)
                     /locus_tag="KPK24_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2298634.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            23059..23919
                     /locus_tag="KPK24_000928"
     CDS             23059..23919
                     /locus_tag="KPK24_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2298635.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(24158..24715)
                     /locus_tag="KPK24_000929"
     CDS             complement(24158..24715)
                     /locus_tag="KPK24_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2298636.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(24905..25792)
                     /locus_tag="KPK24_000930"
     CDS             complement(24905..25792)
                     /locus_tag="KPK24_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2298637.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            25911..27110
                     /locus_tag="KPK24_000931"
     CDS             25911..27110
                     /locus_tag="KPK24_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG2298638.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(27304..29859)
                     /locus_tag="KPK24_000932"
     CDS             complement(27304..29859)
                     /locus_tag="KPK24_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2298639.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWSLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(29856..30542)
                     /locus_tag="KPK24_000933"
     CDS             complement(29856..30542)
                     /locus_tag="KPK24_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298640.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGELL"
     gene            complement(30619..31527)
                     /locus_tag="KPK24_000934"
     CDS             complement(30619..31527)
                     /locus_tag="KPK24_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2298641.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(31529..32200)
                     /locus_tag="KPK24_000935"
     CDS             complement(31529..32200)
                     /locus_tag="KPK24_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2298642.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            32462..33526
                     /locus_tag="KPK24_000936"
     CDS             32462..33526
                     /locus_tag="KPK24_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG2298643.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            33684..34370
                     /locus_tag="KPK24_000937"
     CDS             33684..34370
                     /locus_tag="KPK24_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2298644.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYKDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            34360..35391
                     /locus_tag="KPK24_000938"
     CDS             34360..35391
                     /locus_tag="KPK24_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2298645.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITGDLKLIALLL
                     IYTMVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            35473..36138
                     /locus_tag="KPK24_000939"
     CDS             35473..36138
                     /locus_tag="KPK24_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298646.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            36151..38568
                     /locus_tag="KPK24_000940"
     CDS             36151..38568
                     /locus_tag="KPK24_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298647.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSST
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(38749..39540)
                     /locus_tag="KPK24_000941"
     CDS             complement(38749..39540)
                     /locus_tag="KPK24_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2298648.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(39573..40160)
                     /locus_tag="KPK24_000942"
     CDS             complement(39573..40160)
                     /locus_tag="KPK24_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2298649.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYSNSKSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMEEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(40337..41728)
                     /locus_tag="KPK24_000943"
     CDS             complement(40337..41728)
                     /locus_tag="KPK24_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG2298650.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(41918..43216)
                     /locus_tag="KPK24_000944"
     CDS             complement(41918..43216)
                     /locus_tag="KPK24_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG2298651.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGYPLPIPYIMPLKETLCMS
                     MSRGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(43573..44448)
                     /locus_tag="KPK24_000945"
     CDS             complement(43573..44448)
                     /locus_tag="KPK24_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG2298652.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
BASE COUNT        15774 a         6605 c         5324 g        16797 t
ORIGIN      
        1 ccaatatcat gtgtaaccaa aatagtagac ttattctcat tcttaataat actataaaca
       61 tcatcacaaa ctaataatct agtctgataa tcaagagctg aaaaaggttc atctaaaagt
      121 agaatatctg gatttacaga aagtgttcta ataagcgcaa ctctttgtct catacctcca
      181 gataattctt ttggatacat acttctaaaa tcccataatc cataagtctt taaaaggctt
      241 tcaactctat ctaaagattt tttatctttt ttgccttgaa tttccaatcc aatagttata
      301 ttatccatta tatttctcca ttcaagcaaa ttatcttttt gaaacatata tccaatattt
      361 ccagtaatct ttatatcacc actagttggt tttaaaagat tagtaagaat atttaatatt
      421 gtagatttac cacttccaga aggaccaagc aaagttaata tttctccttc tttcaagtta
      481 aaattgacat cctttaatac ttcaagctcg ccttccttag tgtagaagtt catatttata
      541 tttttaactt ctactatgtt gtcattcatt atgccacccc ctataacttt tagctcgtat
      601 atattatgtt attaacaata tgacctattt gtaccatttt tcataaatat aatataaata
      661 ttttgtttat ttacgtattt tcttagtaac ttgtacgtat aaattataga taaataaatt
      721 ataaggtagg ggataaattt gagtaacata ataacgaatt taatagagtc aatatttact
      781 aatccaataa ctatattgtt tttacttgca ataactgtgt attctgtttt taaaataata
      841 gaaaataaca gagtatattc atttgtaagt tcaaaattag acgaaatcaa tagagactat
      901 aagacaagtg agttttataa aaaggtaagg gatgactact atacatatag taaagaaaat
      961 ccttatgcag acgtaaatat aacttcattt atagaggaag ttgtatctga tttaaagcat
     1021 aataatttgc cattgttaga aaaaattaga agtataaaaa attcatcttc aataagtata
     1081 ttacttgggg ttttaggtac gtttgttggt ctttctacaa tgcttttatg tgttgatact
     1141 aaagacatta taaattcact tccttcaact attagtagta tgcaaactgc atttacaact
     1201 agtatatttg gagttgtatt ttctctaatt ataggttatt ttactaagat aaaagattgt
     1261 gaacatgttt tgatacaaat aatgttaaaa tctgaaaatt tactaacatc tgaaataact
     1321 cattttaagt cagaaagaat ggatttaaaa gtagaagagg taaaaaatac aataaaacaa
     1381 ataagtaagt ctatagaagc tattgaaaaa tttgataaaa tatctaaaga tttaaatgac
     1441 tttaatgatg aatttatcag tggtatagaa gctttaaaaa gccttttaga aggttctcaa
     1501 agttctatta agacatttga ccagagcgta agaaaattag ataaacagtt taatatttta
     1561 aatataaaat ttgtaaagct ttttgataaa tatgataatc aagataatat aaataaagag
     1621 attttatttg atataaaaga atcttcaaaa aatatataca atgccactga aagccaattc
     1681 aaaattagag attatataaa aaatataaat gcaggatttg ctctatatga aagaagtgct
     1741 caagatttat taactaaatt aatgacccat gaaaataaaa tctctcaaaa tcaaaaaatc
     1801 cttttagatg aaaagtttac cttagatgac tcaataaaaa atctctcaag tattattgaa
     1861 aacttttcta atgacctaca agttaaactg gatatgatgt ttgaaaattc tttagatatt
     1921 caagataagt tagatgttat gtttaataac tcttttatga atgatgaagt tcctcttgat
     1981 agtgaagaat tatttaagga tgatgttaat aatgtattta atccttttag tgaagaaatt
     2041 tatgagatag aggataaaga aataaaggtc ataggtgagg atgagttaaa tgaataataa
     2101 atatagaaga actgttaaca gagaatttga aaagagtagc ttttggccaa catttacaga
     2161 tttattatct actgtattga tggttgtaat tcttatctta tttagctcag aaagtatttc
     2221 aggttctgtt gaacaagatt tagctaagaa tgtcaatgct tctgttgaag aaacttttaa
     2281 aaaaagtgga atacctgtga aggtagataa atctaatggg caagtaactt ttggtgaaaa
     2341 aaccatgttt gatgtagata gtgatgtttt aaaaccagag gctaaagaaa tgctaaaaat
     2401 gtttgttcca aagtacatag agactattta taaagattat ggtgactata tatccaaaat
     2461 agttatagaa ggtcatacag atgatgtggg aagttatata tacaacttag atttatcgca
     2521 aagaagagca tatagcgttg ctaaatttat agtgggagat gaaataggtg actataaata
     2581 caaagataaa gtgactaagc atattattgc aataggaagg tcaaaagctg aactcatcaa
     2641 aaatggtgac aatagcgtga atagagatgc atctagaaga gtagaactaa aatacgaaat
     2701 aaatattaat caaaataaat aattaaattt ttataaaaaa aaagaaggca gttggtgtga
     2761 gctttaagcc ttctttaagg aaaaagttta tttatttttg ttgatactta acatattata
     2821 gtttttagaa gcagtttgca atatattaaa cgtaaatggt cgtttaaaat gcataactgg
     2881 atactactga tgtaaaatat ttcattttat ataatataaa aattatacag tatcaatttt
     2941 ctatattatt gtactataat ctattattta aaatattatg taaaaaaata tcataaaaac
     3001 gcatatcatg ttataattac tagagtatta tgaatatgga ggcgtagttt gtgatagaaa
     3061 taagtataaa aaataaaaaa aattctaaaa ttaaaacttt gatactttta ttgatagtat
     3121 ctatatcaat ttatttaact ggttgtacta cattagataa tggaaataat caatcaaata
     3181 ataatgattc tttgtctagt agtatatcaa gtgataagaa tgtaaaaatt cattttattg
     3241 acactggaaa tagtgatgca atattgattc aagatggtaa aacttttata ttgatagatg
     3301 ggggagataa tgatgatgag ggattaatgg tagattacct aaacaatcaa ggtgtaaaag
     3361 atattaaata tcttatagct acccattctc atgcagacca tttaggtgga cttgattctg
     3421 ttgtcaaaaa ctttaatata gaaaatgttt ttgtatcgaa tggttcagca gaaacaaaga
     3481 gttataggga ctttataaat gctttagcaa ataagggttt atccccaagt gtaccgcttg
     3541 aaaataataa gttctattta gaagattctt attttgaagt tctaaataca aatgggggag
     3601 atactactaa tgaacaatct ctagtccttg tatatactaa tgggaatgat aaagttttat
     3661 ttacaggtga tgctgaagaa ggtaccgaaa aagaaatact ccctaagctt gagaaagtag
     3721 accttttaaa agtagctcat catggttctc gtagtagttc atctcaagag tttttagata
     3781 aagtaaatcc tgaatatgcg gttcttttag taggcaaggg taattcatat ggtcatccac
     3841 atcaggagac aatgaataaa ttaaaaaaaa tgggagtaaa agtacataga agtgatgagt
     3901 gttcagatat aatatttgaa tcaacaggtg atggagtatt tacaagttgt aaagatggaa
     3961 gttataataa aggcgttaga gaagatggaa actctggaaa atcaaacagt aatacaagta
     4021 aaaatgattc ttttaaaaat aaacaaaata catccaattc agaagaaata gtgtatttta
     4081 ctccaaaagg taagagttat cactctacaa aaaattgtag tgggctttca agaagtaaaa
     4141 aaatattatc aggcacaatt gcagaaagta agaaaaatga cccatgtgat atatgttatg
     4201 gaaaataaca aaaaaacaac caatttatct ataaagcttc tgaactttaa atcatttaaa
     4261 aaataaaata aatgcataaa aaaataagct ataatcaata gataacacat tttatctata
     4321 cgaatagctt atttttttaa gtatttttta atagctattt aataactcta aatacaccag
     4381 ttacaatacc tacaatttca acttctgagt cattgagcat aattggttcc ataaaatcat
     4441 tttctggctg aagtcttatt aaatttcctt ctttaaaaaa cctcttaact gtagcagatt
     4501 caccatttat aagtgcaact actatctgag aatttaaggc tgtatttttt ttgtctacta
     4561 caacgtaatc ttcatctaaa attcctgcat taatcatact ttcgcctttt actcttaata
     4621 caaaattgtt actgcccttt actaaactgg cagggaatgg aatgtattcc tctatgtttt
     4681 gttgagctaa aatgggttct ccagctgtta tttgacctac taaaggaagc tctattattt
     4741 cttgattagc tccagaaaca tcatttactt tactacgttc taaaacttca atagctcttg
     4801 gtttagtagg gtcctttctt atgtatccca gtttttcaag tttatttaga tgagagtgca
     4861 cagttgaagt agatcttaac cctacagcag tacatatttc tcttacagca ggtggataac
     4921 cttttaatat gatttgagac tttataaatt ccaatatcaa gacttgcttt tcagttagat
     4981 ctaaatacat aacatcactc atttccaaac atattttgtt tttatttcca tacactttat
     5041 atttttaatt ataacataat ccatttatat ctgcaaacat ttgttcctga tttttaatcg
     5101 tcctcaatcc attcaaaatt cttgactata tcacgtttta catttttctt atgagctgca
     5161 taaagctgag tagttgtgac attatcatgg tcaagtaagt tttgaacatc atatatagaa
     5221 aatcctgttt ggattagaga agttgcagcc gttgctctta atttatgggg actatatcca
     5281 ttgtctctag tagtatcaag tggaatagat gtatattttt ttaccagtgt tcttatagcc
     5341 tttgctgtaa ttctcttatt ttgaagagac aaaaataaag catctttttc ttcatcattt
     5401 aatagttcat ctcttgttct ttcgttttgc aaataatctt ttattacatg ttcacaagta
     5461 tgatttatag gcattaaaac ttctttccca cgttttctat atattttaaa ttcacctctt
     5521 gaaaaattaa aagaggagat attgagttct ctaagctcat ttaatctaag cccgtatgta
     5581 acaaataatg ctagtatagc tttgtcacgt aatttagttt ttctccaata taccttttct
     5641 ttctctgtaa gcccttcacc tgtttctact gattcaagca tcttagcaac ttcatctatc
     5701 tcaaggcgct taatagcatc tggctgaggt ttaggaagtt ttattggatt gaatccatca
     5761 gttatattat tatctatctg agagtttctg tataagaatt taaatagagt agagatggat
     5821 gatttttttc ttgctaaagc tctattgtta ttttcaaaaa tcaatgtgtt tttttctgta
     5881 tttttgtaat atctactgca ataatcacct aagaataaat ttatatctct tgattttatt
     5941 gtattaaaat catcttctgt aatatctttt acacaatctg catttgttaa ttctttggtt
     6001 tcaatcaaat atgaacaaaa aaaattaata tcttctaaat aagcaagtcg tgtagaaact
     6061 gcaacagaac cttttaaata tataaaataa tccttcataa atacaggaag tttattttcc
     6121 aaaagtagac atttttctat gagttcattt tctttgataa caatattatc aggtttatct
     6181 gatttattat gtacttttat aattttctta tcattattca aaattaaacc tcctaaaact
     6241 aaaatataaa tcttttatat tattttttga tatttatacc aagtgagtat aaatatttaa
     6301 tttaatcatt ttttacattt ttaagagatt agcagtgctt tagatgaaat tatcactaac
     6361 tattccctaa atccacattt aaagactagt tgaatttaag tactcttttt tatttctcaa
     6421 tatatttata gaaatatttt aaaatatatt gagaaatttt ttatgctcta ctaaaatctt
     6481 ttttatataa tatttttata tgtttctaac gaaaccaaat attagtaaaa tatgtattta
     6541 tttaactaaa gtttttaaat gggctgacta agcttaaaat ataggatatt atgagagtta
     6601 ctattgaaaa aataaaccct attgccataa agaaacttat aaatatgtta tacttaacta
     6661 ttaaactgga tatattcaag ttatcaaatc caaataagta tgtaattgaa cttattacaa
     6721 aaataactat aactataaag ctcagaccat ttaagctatt ttttaaatca gcactactaa
     6781 gtgacatatg tgtagatata gaatacatta aaaacataaa tataaaatac ttcaatgaat
     6841 atacactagg tgttaacaac attattctta taaaattgga aatatcacct aaaataatgt
     6901 gtataaaccc atttatattt aaattattaa tcatacttac atataacttt aaatctatat
     6961 tttgagtcac tttcatataa ctatctggca gtaatatctt aaaaattaaa ataatcatta
     7021 ttgttccacc tataataggt gcaattccta taaagaaatt tcctatactt tgataaagat
     7081 tatctttttt atagctatga cttacatatc ctagtatacc atcattttta ctttctattg
     7141 gtctgaaaaa cttaactgaa tttattttat gcatgaaaat taaacacatt atcaaatgac
     7201 tcaattcatg aacaaacgtt cctatagctc cagttacaat aattccagtt tttccaaaag
     7261 atgaaaatat aaatttatta tttagatttt ctgtaatgct aaaaatcaat ccaaatacaa
     7321 taaatattcc tatgaaacaa aaaatattta ttacacttat ttttaaaatt tcgattatat
     7381 aaatcataag ttaagtcctc atgtaaaagt atttaactac tctacttcat atccattaca
     7441 gcttaaaatt ttacaagcat tttctatatc tttttctttt actaagatat agtctgtatc
     7501 ataagtagaa attgcaaata tgcttatttt gttgtctgct aacaaactac ttaacttaga
     7561 taaaattcct acaagtgaaa agtctaaagg acctagtatc ttaagtattc tccactcctt
     7621 ttctgacttg acatcagaag gaatattatt atttaaacat acaacactta attcatcatc
     7681 agttctagtt atagagtaaa acttctctgt atctatccat gttggtattt tactgtcatt
     7741 atttaatcta catactgcat actcttctac caataatttt aatttcataa tgtatatttc
     7801 ctccaatctt atttgtttac ttaagattat acattaaaag ttacataaaa taaaacatat
     7861 aaagtaaaaa aggttcacta tagtgtccta tttctatagc caacctttaa aacttaatta
     7921 aattctaata aagagatata ttatctaggt tcaattataa gtttaatggc tgttttctta
     7981 tcaccttcga tttctatatc agtaaatgcg ggaatgcaaa ctaaatccat tccactaggt
     8041 gcaacaaaac ctcttgctat tgcaattgat tttatcgctt gatttagagc tccagctcct
     8101 atagcttgta tttctgcaac gcctcgttct cttagtactc cagccaaggc tccagctaca
     8161 gaattaggat tagattttga tgaaactttt agtacctcca tttcaacatc tccttgttaa
     8221 tttgttaaag tatccattac ctaaagtact aaatcataat tgccatttac taattacaat
     8281 catcatttaa agtatacttt tataaaaata tacttctata atcgttttaa gaaaaccttt
     8341 atttttttta accattttat tatgaaaaaa ctacaaattt ctttatattc tataagcatt
     8401 ttccaatatt tctaaatttt ttatatcttt gagctataat ttcctcttta tccatatctt
     8461 tgtaatgatt taatctttct aaaatataag cttttatttc ttttgaaaca gcttctacat
     8521 ctttttgtgc tcctccaaga ggttctttta taatcttatc tattattttt aaatcatata
     8581 aatcattact tgtaagcttc ataacatcac aagcttcttt agctttagat gaatctttaa
     8641 atagaatact agaaagtcct tctggagaaa ttacagaata aactgaatgt tctaacatac
     8701 atacatcatt tcctactccg atacctaaag ctcctccact accaccttca ccaattacaa
     8761 aagtgattac tggaacagaa agtttactca tttcaagtaa gtttcttgca atagcttctc
     8821 cttgtcctct ttcttctgct tctagaccac aaaaagctcc tgatgtatca ataaacgtta
     8881 ttataggtct attaaatttt tcagcttgtt tcataagtct taatgctttt ctgtatcctt
     8941 ctggatgagg cattccaaaa tttcttttta tattttcttt tgtatctctt cctttttggt
     9001 gtccaactat agtaacatta aactttccta tctgaccaat tcctccaacc atagaagggt
     9061 catctttaaa aagcctatct ccatgaagtt ctaaaaaatt agaacaaata tgttctatgt
     9121 attccaatgt cgtaggtctc tttatatctc tacttaatgt taccttttca tatgctgata
     9181 gatgtgaaaa agcatcttcc ttaagctttt ccaatttctc attaagtgaa tttattttat
     9241 cacttaaatc tatattattt gccttagaaa tagttattaa ttgtttaata tcattttcta
     9301 agacttttat tttttcacta ttattttcaa tcaaaacagt tcctccttta tgccctattt
     9361 cttgtgcata gcaagaattt gatataatac ttccttcatt tttcttctat ctactatcat
     9421 gtcaataaaa cctttttcaa gtaaaaactc agaagtttga aatccttcag gaagtttttg
     9481 atttattgtt tgctctatta ctcttggacc tgcaaaacct atcaacgtat ttggctcaga
     9541 aattataata tcaccaagca tagcaaaact agcagttact cctccagtag tagggtcagt
     9601 tagaactgat atatatggca ataatttttc ttcaagctta gaaagtgctt gagaagtctt
     9661 tgccatctgc ataagagata ccataccttc ttgcattcta gctcctcctg aagcagaaca
     9721 aataattata ggtaaattat tttcagcagc atactctatt gaataagtta ttttttcccc
     9781 aacaatagaa cccatacttc ccatcataaa atcaggattc attacacaaa taactgtttt
     9841 aattccattt attcttccat aacctgtgac aacagcttct gactcttttg ttttttcctt
     9901 ataagagtct actttttctt tgtatttagg aaaatttagt ggattttgaa attctacatc
     9961 agaattaaac tccataaaag ttcctttatc aatcaataaa tttattcttt ttcttgctct
    10021 taagccataa tgtcttgaac attttggaca tacaaatgaa ttttcttcta tgtcctttct
    10081 aaatacatga ctatcacaac ctttgcaata ggtccaaaat ttatcatcta cactattctt
    10141 tttaaagttg tcatctaaag aaatcgtaac atacttagat tcttttgatg ataaaaattt
    10201 ttttatcata atctatttac tccttataat gtagcaaatt ctttcttcat aaagacatct
    10261 tctataaatt tggtatgatg agtatcttct ctaaactcct cacttgttac aagtgccttt
    10321 tgaaattcta catttgtatt aataccttct acaataattt cgtctagagc tctatatatt
    10381 ctacttatac attcttctct agtttcagcc caacatatta acttacctat catagaatca
    10441 tataaaggtg gtatagtata tcctgcatat acaaaagtat caaatctaac accaaaacct
    10501 ccaggtaaat ttaatgattc tatcttacct ggtgatggag caaaattctt tgatgaatct
    10561 tctgcattta ttctacattc aatagcatga cctctaaaag taatatcttc ttgtttaaaa
    10621 ggaattttat ccccattagc tatagatatt tgaagtttta taaggtcaag atttgttacc
    10681 atctctgtaa ctggatgttc aacttgtatt cttgtattca tctctataaa ataaaagttt
    10741 ttatctttgt caactataaa ctcaatagta cctgcaccga tataacctac tccttttact
    10801 atatctactg caattttatg aagtttttgt ctttcttcat ctgaaaggta tggacttaaa
    10861 gattcttcta ttactttttg atttcgcctt tgcatagaac aatctctatc acctaaatgt
    10921 attgcatttg aatgtttatc tccaaatact tgtacttcta tatgtctagg attttctata
    10981 aacttttcca tatataaatc actattagaa aatgctgcta gagcttctga acttgccata
    11041 tcaaaatttg aaaaaagttc ttcttctttt ctaacgattc tcataccttt tccaccgcct
    11101 ccacttgagg cttttatcat aacaggatat ccaatttctc ttgcaacttc taaagcttct
    11161 tccttagttt tcaaaacaga ttctgaacca ggaacaacag ggatattcaa ttccatcata
    11221 gtttctcttg ctctagattt atctcccatc aaaatcattt gttctcttgt tggacctata
    11281 aaaataatat tattatcttc acattgcttt gcaaattcag gattctctga taaaaatcca
    11341 aagcctggat gaatagcatc acactttaat ttaagagcaa gatagattat attttctata
    11401 ttattataac tcttacttat attatttggt cctatacaaa tagattcatc agctatatat
    11461 ctatgaaaac aatctttatc tatttcagaa tagatagcaa cagtttttat acctagttct
    11521 ttacatgttc tgatgattct aactgctata tcgcctctat ttgcaactag tatttttttt
    11581 atcaacttaa atacctccta ctacaatggt tttatcttaa ataaaggttg attatattct
    11641 actagttctt cattactaac taatacttca attatttcac ctttaatttc acttgttatc
    11701 tcattcataa gcttcattgc ttctaatata cataaagtgt cgccttcttc aactacatcg
    11761 ccaactttaa caaagctatc tgcatctgga cttggagaat catagaatgt tcccatcaaa
    11821 ggagctacta ctactgatag attctcatta gatgtgacag gtgtatcgtt tacaactggc
    11881 ttttctaaag aaagatccac tactgattct tgttgtacac ttaaaacagg tgaacttgta
    11941 gattgagctt ttttagatac ttctaatctt aaatcgctac tttcaagttt tacatattct
    12001 aagttagttg agtctatggc ctttaataat tccttaattt cattgatgtt cattttgtta
    12061 cccccttgta ttattacctg gtgctaatat tatatcacaa aaaacaaatt tataaaagaa
    12121 aaagtgctga ctagagaatc aacacatcaa tatatctttt aattttaaat taacaaccag
    12181 taacttatat ttgttttaat tttccattat taatataaat tataagtata aaatgaataa
    12241 aattcaatat tttttgcaaa atacaagaga atacaaatta atacctcggg aatttttaag
    12301 acaaatacca atattatgca atttagactt cgctttcttt caaattagta tcattttctt
    12361 tatttttaca taggatgtac tttaatttag ctatcaaaac attgctatat aggttaaaag
    12421 ttattagaat tgcaggaatt ataaagtaaa ctctagcacc tataaaacca aaaactactg
    12481 taataagtat taatctttta tttaaagtaa aaaaaggtat attaaaaata cttttgtcgg
    12541 atgtttctgg agtataagaa tatataactc caaaaatcaa agtaagcata caaagtggaa
    12601 gtagtgtata acttgaaatt attcccagaa atcctatact ttcaaaatat ttgctagcac
    12661 tatttgtata aacaaacatg tatccattta aatatgcgca tatacatgat ataatgccaa
    12721 aagttccccc taaggattga gtgtaccaat taaaaaattt agattccata tacctcacct
    12781 cctaaaactg tatatataat gatatgcatt taaatagtaa aatatatctt tatatgtttc
    12841 tgaataaaat tttgttaatt tcattaattt atgtttaaga aatttgaagt atagtgtagt
    12901 atatattaat gtagatgaag ttttatagta cttatttcat agtgcttaat caagttatct
    12961 tattatggat tcaaaagttg tttgtagggt tttatatttt gttgagctga aaaatttatt
    13021 ataaatgaag ggatgttttc gctttggtag atacattaga atttggtctt aaaattttat
    13081 tttttatatt atcaataatt tggatgggta aaataatgat tttaagaact gataagcaaa
    13141 tagtaatcaa tcctttgctt attggtatat ctgcagtcct tgtgatgttg catacctcac
    13201 agagtaacat tgaatttttt ggattagatg tccaatatat aagaatagtt ttgtatataa
    13261 tatattcact gataatttta ataggaatat gggctactaa cagaagaaat ggaatttttt
    13321 agataatcta tttctataaa atttaaaggt tgctagtaac aaagcactaa tattagcgtt
    13381 ctgttactaa caacctttat tttgattata cacttaaatt gaaaatagaa tgattgaata
    13441 agttgacaga ttattttaca ttgacagtaa ttagaacttc ttgagatgta tttaaatcat
    13501 ttttgtcgct tagtataact tttaccactg cttgacctgt tttattacct ttaattatca
    13561 tatcttcaca tgaaagtaca ctcttatcac ttacagatac aataaaattt aaatcttttg
    13621 gaaggttatc agtaacttcc cagtatttat aataactatc aattttcttc gtaattattt
    13681 ttctcaaatc aatactttca ccttttgcta tatcaacgtt atgatattta gagtccagtt
    13741 tatactgatt atcttttgta tcaataaaat ttaaatcgca atttacatcc acaccattat
    13801 gagaaagaga atttactaca tcgtcatcaa tcaaataatt attggcaaga tttattgaat
    13861 ccatatcttt tagacttaaa gcttccttag gaatctcttt tatattatta ttccaaaaaa
    13921 taaaattgtc tacattttca agatatgata taccttttaa atttgaaatg ctctcaccag
    13981 ttgcataaaa ttctccatct aaagccttta tattgcttat agtcatctca tctggattta
    14041 tatatctttt tacaactgct cttaaatttg catcaggaaa aatatcttta agttgatttt
    14101 cgctcaaaac cttatcattt gaatttattg gacttaaagc tgctgcagaa ataataaatg
    14161 gtattaaatt agctaaattc atagtaccct cccacataaa ttttttatta taataattta
    14221 atttctatga gagggttctg tttccttgtt atatatacct actttcgtac actttattaa
    14281 tataaattaa aataaatttc tactattaaa cttataaaca tattactagt tacgtttttt
    14341 agcttcacat ctaaatattc gcataccttg tgacataaat ttgtcttcat actctgttgt
    14401 aacattattt tcatactcac tatttcctaa atcaagagaa atattcttta atagccaatt
    14461 atttgcagca atttcattta aactaaactc gaataatttt tcattatctg ttttaaagtg
    14521 aatttcgcca tcatcttcca aaaccctatt gtacatttct aaaaatccag aatgagtgag
    14581 tcttctttta ctccatctat ttttaggcca tgggtcacag aaatttatat aaattctcga
    14641 taattcttta gcttcaaagt aatccaaaat attgcttaca tctccccata aaaatagtat
    14701 attattcaaa ttagaagcat ctgctttttc aacagccttt aaaagaactt cttccttaag
    14761 ttccatggca atataattta tgtcagggtt ctgcttagct aaagttgtta taaacttacc
    14821 ttttccagtc ccaaattcaa catgtattgg attatcattt ctaaacttta aattccactt
    14881 gccttttaat ttgtctatat cgtctcttaa aacatatttt gtataactta aaagcttttc
    14941 atctgcacct ttttttcgtc ttcttctcac aataattctc cttacatata aaaaattagt
    15001 aaaatacatt tttattatac attatctcac caaaaaataa aaagaactct cttaaaatag
    15061 aaatttattt ctataaagag agttctaaac ttactaaaaa cttaatctta gtggcaccct
    15121 gaacaaccag agcatcctcc tccaccttga tctttcatat tttcaggaat aactctaaat
    15181 ccaaatcctg tatcttctat tattatatct ccatactgac tatattcatc agagctcatt
    15241 acaaattgta attctccaac ttcataagtt aagtcatctt cttttttctc atctaaagct
    15301 aatccaaaag aaggacctcc acaaccaaca cctgcaaagt atactcttat attattaggt
    15361 ttatcttgat tgtcttttaa gatacttttt aaagtatcaa tagctgtttc tggtaaagta
    15421 attttcatca aatcattccc tttcaaatat tttattaaaa caatatctat tatataccca
    15481 tacaaggtac ataatcaatt agtttttgca tattttacta aaacttctac ctaatattac
    15541 ctaaggcttt aaaatatttt atataatatt ttattacctt aaataaatac cctatcataa
    15601 acttagtaaa caaataaaat tattattcta aaaaatataa taattttatt tgttgtttta
    15661 atattataac acatttgata atgtccaatt catatatgca aaatttatat actttaaaat
    15721 atctaagaaa ttattcaata aaaaaatact attttttaaa aattatcatt attgataaag
    15781 ctctactatt tatatttttt agtgtattca tgtataataa gtaaaagcac aacggtatat
    15841 actgttgtgc ttttttgtaa ttaataaaca atattttggt ctataacaat gaaaaatggc
    15901 tactttccat aattgatttc atctgacata agcaaaaagg gtgtccagct gggtcaatca
    15961 taactctcca gtcatcagta aattgctcct ctgcgattgt agctccacaa tgaattgcat
    16021 attgaactgc ttcttctaaa tcattaacag cgaaatccat atgagccatt tgctgttgag
    16081 attcaggttt ttctggttct attatgcccc aagcctttgt tttttgcaat ataaagaaaa
    16141 aattatatat tttaatattt tataattgtt gaaattgtgt aataaattca actggataca
    16201 tagtgcgttt tgaaatttct tctaaagcta aaccttgttt taaaagcctc tttatgacgc
    16261 tttccatact ttctgaaatt tctataatat ttttatcaaa atcataaaac ctaaaagttc
    16321 tttgacccca tgggtattct tttatataat gcaaaaattc aatatttcct attctattta
    16381 tcttactttc ccattgtttg atgtcattaa cctcaaaata caactgaaag ttatttgatt
    16441 gttgatatat tggtaaatca tatccaatta tagctttata attactttgt aatccaaatc
    16501 cattttcaaa tgatacatgt tctcctaaat ctaatgtgat tttttgctcc ataacatctt
    16561 cataaaactt ttttgattta tttatgtctt tgacaattag caatactcct ccaaatttca
    16621 tcttatcact atcctttctt tgttataagt attataactt aaaattttgc aatttcactg
    16681 taaaaatcag acatattagc tcggtatttt tgaggagtta ttccacagac aattttaaaa
    16741 tctttaataa aatgtggtat atcatagaat cctaattcat tacatataga catcaatgaa
    16801 gttttattat tgtttagaag ttttatagat ttattaatac gtaccaaacg agaaaatgac
    16861 ttcatactca accctaggta ctgattaaag agtctgttta aatgcctaga actataaaat
    16921 acttttttag aaatttcttg tgaagatata tttccagagt tttgaataat caactgaatt
    16981 gccaaaataa actcatatga gtattcaatt ttaatattag aaatcaataa ttcttccaac
    17041 tttaaaatca atttatctat agataaagat tcattaaata tttttttaat ttctaaatct
    17101 aatgaagtat taattataga aaatggaaca actttatcaa taagttcact ttgctgtata
    17161 cctataagcg ggaaaaaacc agcaggttga aattctataa ttagtataac atcacatcta
    17221 ttagcaatat ttcctactgt ttttggttta gtagttggac caaataatag actatgtaac
    17281 cctgtggcat cataaaaaaa caccaaagtt acacttccat gaggaataat cgtgtaatta
    17341 tcagaaatta ttgtttgatt tggaaacgat atagtaaaat tcgatatcca atttcttaat
    17401 tgtatacaag gcaagagata aacaaaattg tcttcacaaa ctataagtga cctattttta
    17461 tttataaaat gaatactttc tttttttaac atatattata tcctgtccta tcgtgaatat
    17521 ttattaaagt aagaagaatc catattatct ctatatatca ttaactgctt ttaataatag
    17581 agatagtatg aatactcatt agatatttaa taaacaaata aaattaaaat aatcaataaa
    17641 aaatatagta cttttatctg ttattttaat attatagcac atttatagta tttaacttat
    17701 atccacttta catatgtcta atttatataa ttaaaaatgc ttatctcaaa tacaataatt
    17761 agtccttata caattaaacc atctgaaact gtaatagtac gactactact tttggctgct
    17821 tctggtgagt gagttaccat aattatagtt tgtccattgt ctctgtttat tttattcagt
    17881 aaatctatta tttctatccc tgttttacta tctaaatttc cagtaggctc atcagcaaat
    17941 agaatctctg gttttccaat taaagctcga gctatagcaa cacgttgttg ctgaccacca
    18001 gataactctc gtggtgtatg tttacgtcta tcagttaaac caactatatc taagatttca
    18061 tctaattgat ttttataatc ttttaaattt ttaccatcta ataacaaagg aagcattata
    18121 ttttcctcca catttaaatt tggtattaag ttataaaatt gaaatacgaa tcctatattt
    18181 ctacgacgta ttatgctcat tttctcatca ttaaaatgtg atatatcagc tccattcata
    18241 tacacttttc cgctcgttgg agtatctaaa ccacctaaaa tatataaaag agtacttttg
    18301 ccagaaccag attgacccat gatagaaaca aattcacctt tcattacttg taaagatacc
    18361 tcttttaaca ctcgagttgt tgtatttcca attttatatt ctttgataat attttttgct
    18421 tctacagcta aactattatg tgtaattttc atattaacct ccataattat tcaaacttaa
    18481 tttcttctat cagtttcatt ttcttccctt ttataatagg cactatcgaa cctattaaag
    18541 ttactataat tcccaataac ccaacaataa taaatgtttt aaaatctaaa tctggtttca
    18601 ttgaaatttt tggtcctgca actataaaga tagtttgaat ctcaagtata gatatcacaa
    18661 taccaataac agctccaagt aaacctgaag taaacccttc aatcaatgtc acttttatat
    18721 tttgtttatt acttagtcct atagatttat acatagctat agaacgtctt ttttgaatat
    18781 aattaatcag tagattatta ataataccta ctgtagcaag aagaaaaatg aaataggtca
    18841 tcttattcat aggagacaaa aaactactta tagtatttaa agaatcatta ttaaattctt
    18901 caactgtacg actccaatta tatgtatctc caaataaata tcttatctga atcataacag
    18961 catctggatt gactgctgta tatactaaaa aaccataatt agttttatca aaatcattaa
    19021 ctgcataatg tgatggtata acagcttcta catcatttgc gcgggattta aaactaccta
    19081 ctattttata agaaaaatca tacttatcat tagatagagt tattgtatct cctatagata
    19141 agcctacttt ttttagagta tcttcattca aaataacaga acgtttatca ttaaaagctt
    19201 ctattacttg tttttttatc tcaaaatttg tataattgat tccaaacata gagttatata
    19261 gttttatatt atctgtaccc tctagtcttg ataatgttac accattacca gaaatctcat
    19321 tattcataac ataaagaggt aatatttttt taattccatc catatgtcta acatcttcaa
    19381 taaactcttc attcatcttt ccatctgcaa aaccttgtaa ttctgcgtca cgaaacacat
    19441 ctgttatata agttttgaca aagttaccaa caacacttat tgcaataact gcagaaatac
    19501 ttataaatag taaagtgata ttctgtatta tatttttgtt atttttcata tttctagcag
    19561 ctaatttacc ctcattccct aaaatatttt tataaacaaa ctcaaaaact atagacatga
    19621 tatcagtaat aagaggaatc aacacaatag tagctacaat taatcccagt aatgaaaatc
    19681 caccagctag atacaatgta ttttcagggg atattcttgg aagtaaaata gataaaataa
    19741 acattatact tcctataaaa agtatagaac gatttgaaac attcttttct tctactgttc
    19801 ctagtacaat attttttatc ggcaaatggc tagctttttt aacaggtata tatgcactaa
    19861 atgaagaaac gattattgct actataacag atataatcac tccaatgggt gatattacta
    19921 ctggaattga tattccttgt tcgagagaat ttcctaaccc atgaagcatt aaatttaaaa
    19981 caactacacc aataggaata gcaattaatc ctcctatact tccgtataat atactttcaa
    20041 gcatcaatat acgagttaca gttttttcat tggcaccaat acttctaaat gttccaataa
    20101 aaggaagacg ttctaatgta ataaccttat aactgctgta tattataaat atactaaggg
    20161 ttaatgcaaa aaaacttatt aaaaagaaag gcattgtctt ttgtctagca tctgaaatta
    20221 tcttagtttc atttatagta tttgatacca tatatttttc agtagaaagt tctttggata
    20281 atttatttac tagattttct gtaatttctt cttctgactc tataagaact cttgtatatc
    20341 cactgccttt atttataatt tttgatagag tctctttggg tagcagtgca tttacacctc
    20401 ttgtgttcct caaaaatact gtatcataag atgcaatatc actaacttga aaagtataag
    20461 actttccata aatctgcaaa gtaatagaat cacctttttt tattttatat tttgatgtaa
    20521 agcgatttgg aagaataatt ttatcaccag aaaaatcagt aatactatcg ccattttcta
    20581 atcgtggctt gttgatttta tttagttgac ttaagtctgc tgatataaga ctaaattctt
    20641 catagtatcc ccctttatta taaatagcag agctttccaa tacaccaacc tttgatttta
    20701 tagcatttaa atcaggtatg tcttctaaat tggttaatat atctggattt ttactttgga
    20761 cagatatagt cgctgtacca tacatacctt ttgccatttt tctttgtgca ctttcataag
    20821 ataatccaat tgagaatgaa acaaaaagta aaactgttga taaaactatg gacaataaca
    20881 taactgctgt tctaattttt cgttctttaa tatttgtgaa aatatacttt aatataattt
    20941 tcaaatttac accttctttc cttatctaag atatttcaat ctaatatttt tatactttaa
    21001 tctaaattaa atctctaata gactcctcca tacttttatc attctattta taatattttt
    21061 aatcaaatca ccccttaaat aaaaaagatt gataaactac tatataaata taataatcta
    21121 tcaaccttaa tatatttttt atttgacctt aattaacctt aattaaatag gaaaggtgat
    21181 ataaaatgag ctacctaatc caggtatact ttcaacagat atattccctc catgaacttc
    21241 aataatttgc ttcgttattg ctagtcctaa accagttcct tctatttttt gttttgtatt
    21301 gcttcctcta taataacgat taaacaaatt tgatgcttct tctcttgaca tcccttttcc
    21361 attatcagat ataatgattt caattctttc atttaatgtt atatgtacac aaactttagt
    21421 atttttatct ccatatgtaa aagcattgac aataagatta ctaaaagctc gttttaatag
    21481 tgtacaatca aaatcaaagt taatattatc tatattactc ttaaattcaa ctatatttga
    21541 ttcatattct ggatagttta aaatatcaat ggataattct ttcaaaaatc ttactaaatc
    21601 ataattattt ttatttaaag ggattattcc attttttaat tgataggtta attttaaatc
    21661 gtttataagt gtatttgtat attctatatt ctttaacatg attgaagcat atttatatac
    21721 tttttcaggt gaataattag tactactatc tgtaagaagc tctgcatacc cttttattgg
    21781 tgaaagtgga gtttttaaat catgagttat atttgaaatc cactcttctc tcataatatc
    21841 agttttattt tgtatctcat cacttctatg aatttcttca tttaaaatat ttaaactatt
    21901 atatatatca gcaaaaacac ctttaatatt aactggaaga tagcttcgtt ttgaaatatc
    21961 gctaacagca gatgtaatat ttgatatgca ctttgttacc cagtaaccat aagcaattcc
    22021 agaaaccaaa attattaaaa atataatccc tatgattgaa aatacaattg acttaccacc
    22081 tataaattta cctccatcaa aaaacatatt cactttagat atatttaatg gaaaatatat
    22141 aatataaata tagtcatttt tattattttt aattgtatta aagtatacag ttccattact
    22201 tgaattattt tggttagcta atgctaataa ttcagaatta gaataataat ctttatgctc
    22261 ttttggttct aaaaacccat aaactctatt tccactagaa tctaaaattt gcaaccacaa
    22321 ttgattttct cgaagttttt ctaatccaga ctgttttact tgtggcttat tatctataaa
    22381 tacgatttgt tcattaaagt tttctgtaaa ttttttcggc cagtcactac gaataaattc
    22441 cccatttggt ttctgaatag taataagtga taagaaaata cctattactg tcaggattaa
    22501 gcaaataaaa atcatgaaaa aaataatgta tgaatgaaat actgttttat aactaggttt
    22561 atgcatttaa tcaactcttt tcttaaactt ttattttgat tattgcttat ataaaataga
    22621 caataattat tgatatgtaa aaatttattt aaaaatacaa ctatctacat aaattatatt
    22681 tatataatta tatgttatca agctatctgt tgtatttaat aaatcctttt gataacatat
    22741 aaaagtaaga taaatttttc cattaaatac cttataatta tacacaaaat ctctatatac
    22801 gttaaacagt atagagaaaa ttttcataga accatcttta taggggatac tttataaaag
    22861 aataaaaaaa ttataatata taaaaacttc ataggagagt aacaacatca gtaaaaatat
    22921 gaaaacacta tataaaaatt caaaaatatt aagaataatg aaatacttga aattgcttat
    22981 atcaagaaat tttatataat ataaactggt caaatatgtt ataatattaa attgtgacaa
    23041 gttcatggag gtgtttatat gaaaaaacaa aagtcgccaa ttttacttga aatgcttgga
    23101 ctattttttg gttgtatatc aatgagtatt ggtataaata tgtttttaaa accacataca
    23161 attgctcctg gaggacttag tggtctatca ttagtactaa acaaggttac aggacttcca
    23221 gtatcagcta tcatgttaat tataggtgtc ccattagtaa tacttgcttt tagaattatg
    23281 ggtacaaaaa attcattaaa aactttattt ggaacagttg tattttcagc tatagttcaa
    23341 ttaacagacc cattatcaaa actaagattt actaatgact tattattgtc ttctattgca
    23401 ggaggaatcc tagttggtat aggtttagga ataatgttta aatcagatgc ttctactgga
    23461 ggaacagact taattgcact catacttagt aagaaattcc caggaataaa agctacaaaa
    23521 ttcatgtcat gcctagatgg aatggttgtt atatcttctg gtatagtaaa tcgtagttta
    23581 gaaactgctc tttactctgg tatagcactt tgtgtattaa taaaaatagc agatatgata
    23641 atggaaggat ttgaccattc aaaagcattt tttattatct ctgatgagcc tgaaagttta
    23701 agacaagcta taactgaaga acttgataga ggtcttacta ttcttgatgg taaaggtgga
    23761 tacactagag aaaataaaga agttctatta gtcgtagttt ctaaaaaaca agaactttat
    23821 ctaaagagat tagttaaaag aactgaccct actgcatttg ttattgttac agatgtacat
    23881 gaggttcttg gagaaggatt taaaaattta gagaactaat aaaaattaat attttaagta
    23941 tatatactat tttaattaaa atacgattta ttcaaaaaga gtgtctcata aactttttag
    24001 ttcatgagac actctttttt gtataaaatc aaatgcattt tcagtatttt aacaataaaa
    24061 acaggattat ctatattttg aggcaaccac tttattgaaa ttttatttag aaaacaatca
    24121 ttactaaata accagcatag atattaaatg attatatcta ttgttttttc aattcatatt
    24181 gataacaagg aacatttttc acagtatttc cagtatgaat ttttttgata tactttcctg
    24241 tacatttaaa tcctaataaa tctaatgatt ttgtagaatt ttggttttct tcataggtta
    24301 ttgcaattat tttatttaag tttagttctc taaatccata ctgtattact ctttttgtta
    24361 tctcagtagc aaaatcatgt ccccaatagc ttggatttat acatatacct atttctgctt
    24421 ctaatgcttt aggatttata ttattaaacc ccatattccc aataacttta ttgtctcttt
    24481 tatatactat agcaaagtgg ccaattttcc cttttttata tggatttaga aaaaatgatt
    24541 ttataaactt tcttgtatct tctaaagatt tatgctcact aaatggtaaa tgttttacta
    24601 ctattttatt tttataacag tcaaaaaaat cttcaacatc atctaatgat gcaggtctta
    24661 gtatacaatt tctagtttct aaggttttca tttgaggata ttcagaatca gacatttcta
    24721 atcccccttt atacattaat ctaaatatac tgttcatatt taaaatacga tattaattat
    24781 ataaatgttc tcaaattaac cttattttta tttttaataa aaactaatta ctatatgaat
    24841 ctttcaaatt tacgaattta tctctatttt tatattaaat tacttgtgaa atatgtattc
    24901 taaattatat attattagat atcatgctta aaaattcttt ggttgctaag gataaagata
    24961 tatcttttaa atggcagtaa cctatatttc tcttaggtat tggctctttt atatctaatt
    25021 taaacaattt ctcattttcc aaataatcta tgctaaattc ttctataaca caagacactc
    25081 caagatttat atacgcaaat tctaaaagta attcatgaga acccaattct atatccgggt
    25141 ttagttttat accttttgat aaaaaatatt catttacata aagtcttgaa tttgctttat
    25201 tttctagcat tattaaaggc aatgtgttta attcttccaa tgatattttc ctacctttgt
    25261 attctatgta atcatttgca catacaaata tatcatgtat ttcaatacac tcaacaatat
    25321 ttagtgtatc atcttctatt ggcatgttta taattgctat atctatattc ccatctttga
    25381 gtaaagatat aagttctcta ctcgttctat ttattgtaga tacatgtata tgtggatact
    25441 tcttatgaaa aatttctaaa tattttaaca agaaaaaacg tgcagctgta tctcctactc
    25501 ctattttcag agaaccgtat tctaattttt taaatttatt tattctatct tctcctgatg
    25561 atattaactt taatgcagta gtgacatgtt cacttagaat tttcccctct ggagttaatt
    25621 ttactccctt attgtttcta taaaataata aagtatctaa ttgttcttct aattgcttta
    25681 ttgactggct tacagctggc tgtgacataa acaattcctt agcagcaaga gaaaaactct
    25741 tattatttac aactgcatta aaaaccttat aaagttcaag ttttatattc atatataatt
    25801 actccttatg tcaaatatta gtattattta ttttacttat aattatattt gaattataat
    25861 atacatggta ctaatatgac aatattaatt ctttaggagg attttagata atggatagat
    25921 tagttggaac tgtttcaaga ggggttagag cacctattat aagacaagga gatgacttag
    25981 ttaaaatagt tgttgattct gttttaaatg cgtcaaaaag tgaaaacttt gaagttaggg
    26041 acaaagatgt aattgccgtt acagaagctg ttgtagctag agcacaggga aattatgctc
    26101 acgttgacaa tattgcaaaa gatgtaaaag ataaatttgg agatgatact gttggggtta
    26161 tattcccaat tttaagtaga aatcgttttg ctatatgttt aaaaggaatt gcaaaaggat
    26221 gtagaaaagt cgtattaatg ttaagctacc cttctgatga agttggaaat cacttaataa
    26281 gtattgatga acttgatgat aagggaataa atccttggtc agatgtttta acagaagaaa
    26341 aatacagaga actatttgga tataacaagc atacatttac tggtgtagat tatgttgatt
    26401 attataagaa tttaatcaag gattgtggtg cagaggtaga aatagtattt gcaaacaatc
    26461 caaaaactat tcttaactat acaacaagta ttttaacttg tgatatacat acaagacaaa
    26521 gaacaaagag aattttaaga caaaatggag ctaaaaaagt atattcactt gatgatataa
    26581 tgacagcaag tgtagatggt agtggttaca atgaccagta tggactttta ggttcaaaca
    26641 aagctacaga agaaacagta aaattattcc ctataaattg tgatgaagtt gtaaacaaaa
    26701 tccaaggtaa tataaaagaa ataacaggga aagatgtaga agtaatggtt tatggagatg
    26761 gagcttttaa agaccctgta ggtaaaatat gggaattagc tgacccagta gtatctcctg
    26821 cttatacaaa agggctagag ggaactccaa acgaagtaaa attaaaatac ttagcagata
    26881 atgattttgc tgacttatct ggtgatgagt taaaagaagc aatttcaaaa tacatagttg
    26941 aaaaagataa taaatctgat gatttaacag gaaacatggt ttctcaaggt actactccaa
    27001 gaagattaac agaccttata ggttctttag cagatttaac atctggaagt ggagataagg
    27061 gtactcctat tatttacata caaggatatt tcgataacta tacaaaatag tatgttttaa
    27121 taagatttaa tttttttgga ttatatatat taacatgaaa atttattaaa acctcttaat
    27181 agatatgact tttcaagagt tatatctatt aagaggttta tttaatgttc acagataaga
    27241 tatatttaaa taatttaaac aagattgtca gaaagttata taatcttaaa aatttatata
    27301 actctaattc ttatttattt ctgaaataat atcactcttc aaaatttgtc ttactggaat
    27361 aattacagca actatactaa tagtgatatt taaaataatc atacctatgt atagaggtgt
    27421 atttatttta agaattggat ttatatataa atatacacgt tttaccataa aataaatcat
    27481 aaattgtcca ataacacttc caattaccat tataatactt gcatagaatc catataaaaa
    27541 accttctctt atcatcataa gtaaaaactt attatccgta atacccatag ctcttaaaat
    27601 tccaaactca tgtcttcttg aaaaaattaa gtagcttact gtattgatta tatggaacaa
    27661 acttattgca agaagtacaa atacaatacc atatataaat aagagttttt gatttaagta
    27721 ggtattattt ttttcaatag caatggtata atctgttacg atacatcttt ttacatcact
    27781 tacaacactt tttatgttct cagacaattc ctcactgtta ctatcttctg tcttttttat
    27841 acttactgaa ttataatttt cgatatcaaa gttatttttc atttgcccat tcgtcataac
    27901 tatatccata ccataatcac caatgaaata tatgttattt gccatcacac gttttacagt
    27961 agcagcaact acaaattctt ttgttgtata atcattctta ttttcttctt taaatttaac
    28021 aatttcatct gttggtttca ttgtctttgg cactttaact tttattgtat ctcctggatt
    28081 tatatcaact gcatcataca tcccagttcc atccataggt agtctaacta taaccttatt
    28141 atttttaacc atatcatggg tattaatctc cccatctata atataagaat ttaatccttc
    28201 taacatacca ttgttataac cataaatatt tgtttttaaa agatagccac cttcattttg
    28261 ctttgtacaa ataccatcaa aaaaagtctt tatcctgtaa tctttttcaa gaggtttaaa
    28321 gaaatttttc caaaatagtt gttcttcttt aatttgcata gctccaaata aatatcttac
    28381 tggatagact tttgatattc cctcaacatt gtttaatctt gaaattttat cttttggtat
    28441 acctacatca aaatctgttg tttgcatagt tatttggtag tcagaattta aaccatcatc
    28501 agctttcatt gtaagttcat tattattttt agtaagctgt atagagtaac tagaacatag
    28561 aaatataatt ccaccaagtg ataatgaaaa caaaattcca ataaacattc cctttttttg
    28621 agtcatgtat ttatgagata atacttttgt catgttagag tgttttacac tttctaatat
    28681 tctctttttc ttattagtaa tcttaaagtt tttcatagat tctatactag agtatttatt
    28741 caactgatgt acaacacgta cagcaattaa aattaacaac aataagataa ataaaaagcc
    28801 taaataaata gatttttcac ttataaaaaa tgctcctgga acaatatcac tactcaaaaa
    28861 tatatgtgga aattgttcat atattgtaga cgctactcca atacctaaga agcaacctat
    28921 aggaaatcca acaataaaaa gtaaaaataa ctctaaaaat agtagaaata aaagttgttt
    28981 attattagcg cctaatactt ctaaaacccc atattgtgat attctctgaa gtattgaaac
    29041 atggaaaata ctatatactg ctgctgcacc aaacactcca acaagcaata taattgcatt
    29101 tgtatttagt tctaaatttt ttaagaactc aaatatatta tatttttcta tattttttgg
    29161 tggttttact ccaaattcaa ctgaaagaga ccagtttata taagtttttt cttttgatat
    29221 gttgaatttt ttcataaatt tataattttg ctttttaata tttttatttt catcatattt
    29281 tacataaact atataagaat ccttttcttt aggctgaaaa taatcgttta caaatccctc
    29341 cagagcagtt tctgggtttg aaagacttga taaaatacca cataacttat agattttacc
    29401 atctaaattg atttctgttc caatttcttt gtttacattt aaattgttta gagaatatgt
    29461 gtcaagtact atctctccat ctttactagg aagagttcct tttaataatt ttctacctgt
    29521 catgtctaaa tatgaagaat cagcatttaa aaattctata ataaaaggtt catctatctg
    29581 atttttaggt tttgtaacgc caagtctatc tacagtgtat tcctttgatt ttttattttc
    29641 tttaatttta ttaagttgtt ccttatctac tttatagaaa taatgataat ctccactatc
    29701 ttcccttatc ttttcaattc tacttttgtc tgcactatga actaaagaac caattcccgt
    29761 caataatgct actgatagta ctatgcttaa aactatagat atagattgtt ttttataatg
    29821 ctttatatat tgaattgaaa attttactag agacatcata gtaattcacc accagtatta
    29881 accacaactc catcttcaat atggataatt gagtcgcata cttgtgctat attttcattg
    29941 tgtgttacca taagtatcgt ttgatggaat tttgcactac tttcttttaa taaacatacc
    30001 acttccattg tagttttact atcgagattt cctgttggtt catctgctaa tataagcgca
    30061 ggtttatttg ccaaagctct agcaattgct accctctgtt gttgaccacc agatagttcg
    30121 cttggaaatt ttaaacgctt atcccaaatt cccaatgtct tcatcagcat ttcaatatat
    30181 tcatggtcta tatggtttcc tttatctaat gtaacaggaa gagctatatt atcataagtg
    30241 tttaatacgg gcattaaact gtagttttga aatataaatc ctatatttct tcttctaaac
    30301 actgtcaaat ctttttttcc tattttagat atgttctttc ctttaattat tacatcacct
    30361 gaggttggat tatctaatcc acctataata tttaacaatg tgctttttcc agaaccagaa
    30421 gttccaacta tagcagtaaa tgtatctcgt tcaatttcta tacttactcc atttaaggct
    30481 tttactttag tctcaccttt tccatagtgt tttactaaat tattcgtcac taatatactc
    30541 atttaatttc tcctttcaat acagatatat gttctataaa atattgtaag tgacgataat
    30601 tacactcatc ctacaaaatt agtttaaact cttacaattc tgtaagaaga tactaaaaca
    30661 acatccactt cctagtttag attctactat aatgtttcca ttttcttttt ctaaaattaa
    30721 tcttgtaaga tatagaccaa tccctgtgcc attttggttt tcaacatttt tacttctgta
    30781 aaatctttta aaaatatcat ttagttctct actatcaata cctattccct catctttaat
    30841 agtaatcttc gtatatagtt ccatttttat tacagaaata gtaatcgtgc tatctgatgg
    30901 tgagtatttt attgcatttt ctaaaacatt tacaattgct tctatggtcc actttttatt
    30961 atgaactaat tttatgtctg aaaatggttc gagatttatc cttatatttt tagcttctgc
    31021 tttattaaag atagtactga tactttgaat aagagtatct ttaattagta gttcttctac
    31081 ttcaaattct ataactccat cctctaattt agtcatttta aataaggatt gtaacagcca
    31141 gtcaatctta aaagtttcat ttttcatctt ttgtaaaaac tccttcttct gtaatgtgga
    31201 aatatttata ttctccagaa gttcacaata aatggttata tttgaaagtg gagtttttaa
    31261 ttgatgtgac atatttgata ttaaaccttt aatcgcttct ttttctgact ttgactgttc
    31321 tacctctatt tctatcatat ccttaatttt ttttacttgg tgagaaattt ttgacgctct
    31381 tgtatccttt gtatctggat aagtaacttc tttaccttcc aatatttgat ttaacatcaa
    31441 atttaattca tcatatcttt ttcgatttat tttagtagat actataaata taacaactgt
    31501 acatattata aaggctaata taaacatatt attctacctc attccataaa taacccattc
    31561 cataagctgt ttttatatat tttggattag atgggtcatc ctctactttt actctaagtc
    31621 ttcttatatt tacagataca atattatcat caacataatt ttcttcgctg tcccaaatat
    31681 ggtgtaaaat ttgttccttt aataatattt gacctttatt ttcaattaga taacttagaa
    31741 gcttatattc tacagagcta agttccaaac attctttatt tttatatact tttagtaact
    31801 tttggtccaa ttttattcca ttggaatata ttactttatt attcgatttt tttcttattg
    31861 ctactttgac tcttgccttt aatacagata aagaaaatgg ttttgtaata taatcatcaa
    31921 gccctatttc taatcctttt acttcatcaa tttctgagtc tcttgcagta agcattataa
    31981 ttggtatatc tgacttttcc cttattattt gacatacatc aaatccatct ccatctggaa
    32041 gattgcagtc taagattata aaatctatat ttttactatt aaatatattt aatccttctt
    32101 gttttgtata agccttaaat acattaaatc catcattctc aaatgcataa aaaagacctt
    32161 catttaagtt tatatcatct tcaataatta ataagttcaa ttttactctc ctttagattt
    32221 tttagataaa taattatttt cttgcattat catactaaaa atattttaat tttatttatt
    32281 tcactaaaaa gattgtattt aaatttgttt tcaaaataaa aacattataa tcaagcatat
    32341 agttatatat ttcccaattt aattacgctt aaattgaaaa tataacttta ttcttatata
    32401 attaaattat acaacaataa agttatattt tttaaatata tttttactag ggggaataga
    32461 attgaaaagg gttgatgaga gaaatactat gtttgcaaga gcaaattaca aaaaagatag
    32521 tactgcttat aatgactatt ataagaaaaa tcctgacaaa aaagaaattg atgatagtat
    32581 aagaaataga ccaaatttat gtagtgaagg aacaatgact tacaatgagt taaactctcc
    32641 aatggcaagt agtgcattcg attttttatc tgatatcaaa tctttatgcg aaggaaaagt
    32701 ttctgatact aaagtagatg tagatgcaaa gtcaatgaca aaaaaaatta agggtcttgc
    32761 aaagcagtat ggagcttctg tagttggtat aactaaatta aaagactatc atatttatac
    32821 aaataggggt agacatgaag aaaattacgg tgaagaaatt aatctaactc ataagtatgc
    32881 aattgtattt ggatgtgaga tggataaaga aatgataaat agagctccaa tgatatgtga
    32941 agttatagaa acatcaaagt gctatgtgga tgcatctatc gtcggcatga ttttaagtta
    33001 ctatataaga aatttaggat atgatgctag aaatcatatg gatgctaatt atttagtaat
    33061 gcctgttttt atagctcgtg atgctggttt aggagatata ggaagaaatg ctatacttac
    33121 aaataaagat tatggctcta gacttagatt aggtgttgtt actacagata ttcctctttt
    33181 agaagatgaa tatgttgatt ttggactaga agatttttgt aaggtctgta aaaaatgctc
    33241 ttttaactgc ccttctcatt cgctttctaa tgatattaaa attggtgatg atggaaaata
    33301 taattgggta attgaacatg agacatgcta tattaaatgg agatatttag gtacagattg
    33361 tggtatgtgt atatctgctt gcccatttag ccaaaatctc gaaactataa agaatactac
    33421 ttcatttaag ggtaataatg agcttattca aaaagcttta gatgagtata cttcaaaatt
    33481 tggtaaaaga atatttatac ctggaaatcc atcttggttg aaataatttt atttaataaa
    33541 gaatctgtac tagaatttat cataggacag gttctttatt aaatatataa atttttaaat
    33601 actttttctc gtttataaca attcagcaat gtttttagtc tataattgac attataaaat
    33661 attaaactaa aaggagtaac tgtatggaaa aaatattagt agtagaagat gattctatat
    33721 taaataaaac tctatcatat aacctaattg aagatggata tataattact tcaaaattta
    33781 cagctaaatc tgcactaaag tctatttttg aatgtgaatt tgatttaata attttggata
    33841 ttaatttacc agataaaagt gggtttgaat tatgtaatga aataaaggaa aattacaata
    33901 taccaataat ttttttaact gctaatgata tggaatgtga tatgattaaa ggctatgaac
    33961 ttggagcttt ggattatata acaaaaccat ttaatatcaa tatatttaaa caaaaaatta
    34021 aagctttttt aaatcatctt acaataaaaa caaaacaaga ttactataag gatggatatc
    34081 ttgaaattaa cttttctgag ttatctgcca atataaatgg aaatcaaatt atatttacac
    34141 cattagaata tcgtacttta aaactgctta ctgaaaatcc taaaagtatt ttaacaagga
    34201 aagtactttt agaaaaactc tgggatatag atgctaactt tgtagatgaa catactttaa
    34261 catctgttat aagtaggatt agaagcaaaa ttgaaaaaga caatcttaag tatatcaaaa
    34321 cggtatatgg tatgggatat atgtggttag gtgaaagaaa tgaattttaa aaaattttct
    34381 tttagttcta tttatttaat aattgctgtt ggtataattt ttacagctat tttatttagt
    34441 atctcaattt atctaataac tggtgattta aaactaattg ctctattatt aatatataca
    34501 atggtacttt gtggatttat agcattaata atatttttac ttcataaaaa aatagttata
    34561 ttttgttcta aaatatgtca cactttagat agtatgatga atagtgaaga taatattgaa
    34621 attgaattag aagaagaaac tattttatca cgtatcaatc acagattaat tagaatgtat
    34681 gaagttattc aagaaaatcg caatagtatt gctatagaga aagctgattt acaagaacta
    34741 gtttctgata tatctcatca aattaaaact cctattgcaa atttaaaaat gattaatact
    34801 actctactaa atcaaacatt taaaacagaa attcaaaagg attttttaat ggatatggaa
    34861 aaccaattag ataaattaga ttttttaatg caatctatgg taaaaacctc cagactagag
    34921 acaggtataa tcacactatc taaaaagaaa aattcaattt atgaaacaat tgctacagca
    34981 cttagtggaa ttttgtttga tgctgaaaaa aagaatattg aagttacagt tgattgtgac
    35041 ccaaatttat atataattca tgacaagaaa tggacttctg aagctatttt taatatatta
    35101 gataatgctg taaaatacac aagttctaat ggtaagatac gtgtagctac agaatgttgg
    35161 gttatgtata caaaaataga tattattgat aatggaaaag gaatatcaga aagtcgccaa
    35221 gcagaaatct ttaaaagatt ttatcgtgaa gaagatgtcc atgacataga aggtattgga
    35281 ataggtctct atctttcaag aaaaattata tctcttcaag gtggctatat taaagttaca
    35341 tcagatattg gaaaagggtc gactttttct attttcttac ctaataaata aatttttaaa
    35401 aatttcattt gtaatgattc tgcaacattt tggttttata ctgtatttac aagatgttaa
    35461 ggaggtatta ttatgagtat tttacaagta attaatttaa agaaatatta tggacaaagt
    35521 tcaaacatta caaaagctct tgatggtgta actctatcta ttgaaaaagg cgaatttatt
    35581 tctattgtag gtacatctgg tagtggcaaa tccacattat taaatatgat gggaggatta
    35641 gataatccta catctggaaa ggttattata aagggtaagg aattatctac tatgtctgat
    35701 gaacagctta caatctttag acgcagaaat ataggtttta tatttcaaaa ttataatctt
    35761 gttcctttac ttaatgtata tgaaaatatt atcttaccaa taggattgga tggagagacc
    35821 atagataagg attttataaa tgaaattata aatatgcttt ctcttgaaga aaaattatat
    35881 gatataccaa gtaatctttc tggtggtcaa cagcaacgtg ttgctatagc tagagcactt
    35941 gtaacaaaac cagctattat actagctgat gaaccaacag gtaaccttga tagtagaact
    36001 agttcagatg tactgagttt acttaaagtg acaagtaaaa aattcaacca aacaattgta
    36061 atgattacac acaataatga aattgcacaa ctaactgata gaattattcg tattgaagat
    36121 ggaaaaattg ttaactagga ggtgtagtat atgtttagaa ataataatcg cacatttata
    36181 agaaaaatag ctttaaatga tttaaaaatt aataaactta aaacatatct ttcaggtatc
    36241 attataatga tttctacatg tttattatta acagtgacct tagtttcata taatgcctct
    36301 gtagatttag ttaatgcatc tccatatcat gctatatata aatctgtaga tgaaaaagca
    36361 aaaaatatac tatataaaaa taaaaacttt agtggcgtag gaacttacaa attaattgga
    36421 agtaataaaa agacagattg tattatgtcg attgtttatg cagatgatac agcaattaaa
    36481 cttatgaatt ttcaaccatt gaaaggcaaa ctacctacaa ataaaaatga aattgctatt
    36541 tcagaaaaat atttacagga atttggttta gataaagata ttggagatag cattaaatta
    36601 aattattatg atgatattac gaataaagaa gttcaatgtg attttattat tgttggattt
    36661 ctagaaaatt attatcaaga taatgccaag caatactata cagtcgtttc aaatgattat
    36721 tttaagaata tagcttctac acctttacaa aataaaagta atacttttga cgagtcaaga
    36781 cctgatactg tagatgtatt agttaaacta tcagaagaaa atacagagaa agattcagct
    36841 tcaattaaga cacagttaaa taaaattggt ttatctttag gtattaagaa atataatata
    36901 tacttaaatg ataactatat tgaatcaaat ttaattgatg gtgagcagat tattacagtt
    36961 ttagtagttg gaatagttgt tcttttttct agcgtttttg ttatatatag tattttttat
    37021 atttcagttg ttaattcagt tcagatgtat gctaagttga aatcgttagg tatgacttct
    37081 tttcagttaa aaaaaattat ttctctacaa ggaaatattt tgtcaattat ttttattcca
    37141 ttaggggtta tagcttcttg tataattgct tacataattc aaccattagc atggcaaatg
    37201 atagctgatt tatttataat tttaatttta tcactggtaa tgtttttaac tgttagaatt
    37261 tctttattta aaccagctag aattatttct aaaatatctg ctattgaagc catgcaatat
    37321 acagaaacta aatttagaaa aaaagcaaaa aattttagtt atataaatat aaaaaatcta
    37381 gcattaaaga atatcgagtc aaatagaaag aaaaatttga ttgcattaat atcgctaagc
    37441 attagtggag ttttatttat ttcaatcgct aatcttgcta attcaataga tttcaaaaaa
    37501 caactatctc aacaatttgt ctataacgaa gattatataa ttagcattaa gagtgataac
    37561 ttatatgagc atattacaga aatccaacaa aataatccac taacagattc tcttaagaat
    37621 gaaattaaat ctattcatgg agtaaaaaaa ttaattgaat ccaaatcaat taaaagtaat
    37681 attatagaac cttacatcaa agatagagac gaaaaacaat ttattactct aattaaaggt
    37741 attacgcctg aactttcaaa atcacttgaa gaatatattg tcagtggaaa aatagactat
    37801 acacatttag attcagatag tttaattata aataaatata gaacaaaata ttatggatta
    37861 aatttaaaag ttggagatac agttacattt aatatcttta gtggaaatga tattattaaa
    37921 aagaaaatga aaattatagg aatcattgac aattccaata caggaggtat gttttttgct
    37981 tcagacaaaa ctttagaatc tctaacacca tataatacaa atttagattt ttctatttta
    38041 atagataaaa agcattctaa agaagttgaa gaaaagttgt taagtataat tcagaaaaat
    38101 tcaaatttaa tgctttattc atatgaagat gattatagaa tgattactag agcatttcaa
    38161 tatataatta tagcttcata catttttgtt ggtttaattt cttgctttgg tatactaaat
    38221 atgaccaata ccctaataaa cagcgtttta attagaaaaa aagagtttgc attacttcaa
    38281 gccgttggaa tgacaagaaa gcaactgcga aatatgcttt atagagaagg tctaaacata
    38341 agtatcaaag ctatatgtac ttcaagtaca ctaggttatt ttggttcaaa tctactttgc
    38401 acttttataa aagatgtaat tagattagac tttattaatt ttaaattttc tatatttact
    38461 attttaattt tttcattcgt attaattggt atacaagttt tagtgactga attacttgta
    38521 agaaatatag aaaaaaaatc acttacagaa cgcttacgtt ctcagtaaga atgtatataa
    38581 aaaatagcca ttttttatac aagttttaat atgatttttt aaaagctata gggatagaaa
    38641 attttgactt tacataattt tctatcccta tacttgtttt tattttatac agatacactt
    38701 acttatatct ccgagcaaca tcaccagaga cataaacaag catattcctt attttctaaa
    38761 tatgtcaatt gcatgacttg taacccctac taaatcttca cgttcacaag tagcaaggtg
    38821 atattttaaa tataatgcaa atgcatcatc atccatagag tctactgctt cacgcatata
    38881 taaagccaaa ccatctgatg cgacataatg cagtctagtt acattaaatg cagacatcaa
    38941 atcatcaatg ttttctttac gtacaagctc aaacaaatcc tttggttcag acttagcagc
    39001 aaatgtctgt ggatcaatca aacctttttc aatatattca gatacattaa tatttcctct
    39061 atggaacccc tcgtcaatga gacagccatc agatataaca tatgcagcaa aaaccacacc
    39121 accaggtttt gttacacgaa ttgcctcatg taaagcttgt tgtttatctt ctttgttata
    39181 aagatgatat agtggtccta acagtaacgt aatatcatat ttattatctg ggaaaacaga
    39241 taaatccata gcattaccct gagtaatgct tatattttca tttgattgag tatttttatg
    39301 gaaaacctct atattatgtt cgactaattc cactgcgtca acatcatatc cctgacgtgc
    39361 cagtgtgtga gaataacgac cagttgcagc gccaatttca agcacacaat cacctgattt
    39421 tatgtactta tcaatgtaat gcatagtagt gagaaattcc accattccat gttttaatgc
    39481 aagtcgacta tcttcatcat aagtattata aaagtcaatt aagtattgtt ttgtttccat
    39541 aatttcacct cttctatatt agttaataaa catcaaatag acaaattcca atctggtttt
    39601 tcttgtctta taaaagctgt taatatattt ttcaacattt tttcagcact atatctatct
    39661 aatgttaatt tttttgttgc tgttaagaca gctatgccat gagtataaag aaataaatct
    39721 aaaaatatta cttttgcaca ttccaactct attccaatgc tttccgaaaa aagtcttgcc
    39781 tttttttcat tgtcaatttc tttataaaaa tcatttgcct cttccatcat caaatctaaa
    39841 tcatttataa ataacaactt aaataaatga gtttcttctt gagcaaattc aatgtatgaa
    39901 agcggaagca gtaaataagg acttacactt ttagaattac tataattaac aacatactga
    39961 ttatagtatt catatgcaaa agcaagaaat tcatttttta attcattcat gttttcgtaa
    40021 caagtaaaaa ttggtcgagt ggaacactgt aatttacttg caatactcct cgcatttaca
    40081 gcttcaaatc ccacttctct tgtaatatcc aagactgtat tcaaaatcat ttcttttgtt
    40141 atctttactt taggtggcat atatgttcac ctctccatta aatttttata aaacacatat
    40201 gttatataac tatagtaaca tatcaagaaa ttatgtcaag aaaaagcttt gcattttttt
    40261 gcaattccta aaacttataa ataaaacatg agttgcaatt aatctattaa tagataacag
    40321 caactcagat tttcctttaa atactcttct ttttgtctaa tatttcatat acacaatctt
    40381 ctattgaatt aatatctttt tcttttcttt ttttagaatc aattaaaatg aaatacagta
    40441 taaaacctac agcaagtccc catgttccgc ctttagcagc aattacacct cccataagtc
    40501 caatcaatcc cttctcaaca cctgtattac atagctccat tgatagcttt gtacataaat
    40561 atccttgaac taaaagtgtt actgacaatg ctaatggtat aagaggtgta gcaatctgcg
    40621 caattggata aaaacagata aaaattgctg aaacccatac taatgtaccc atgccactaa
    40681 aaacagactg catggcttcc tttccaacct tgtacctttg agcaacactt cctgtaagtg
    40741 tcgcagctaa tggaccgcac attggtatat atggagaaaa gattgccata aaaatatttc
    40801 ttatcccact tataacatta gacctatttg aattgaaatc tatatattca tcttttctat
    40861 tttcactttc agaaagtact aagttttcac ctgttataaa atcaccaaag gcaattatgt
    40921 atatcataaa agccattggt atcccttgta taaataacat tacacttgga aaacctattc
    40981 caaaaggaga tactgtattt atcagatttt taaaatctac tatgtaaact aaattgtcaa
    41041 aactaaatct aggaatttct atttctttac taaatatacc tactattaaa ctcactaaaa
    41101 tagctgatac tacaccatat tctcctaaat gaaataaaaa tttattctta acttttagag
    41161 tatcaaatct ttcagaaaac attacaaaac aagcaaccaa aattcctact gttatagata
    41221 taggataaga atcaaatctc cctttaggat taaactctga tattatagtc gatataccga
    41281 cacccattag tattccacct ttaatagaat ttggtatctt actaactaac tttccagcta
    41341 caccagttat acccattaaa ataaatatta ttcctaaatc tatttgtaaa gctgttaatg
    41401 cttgtattct ttcaatgccc atgctgaact ttaacaaata tgcagttgta agagttatag
    41461 aagctgtaat ccagccaggt acaactgggt ctcccataaa aacatgcaaa cagtataata
    41521 ttgagttaat gatagccata ctccatgcca cttcataggg taatcccaaa atttgtgtac
    41581 taaggccaat tgtgcctaaa cttgtcatac ttgatattat accttgaata gcttctggtt
    41641 tctcaaatct gtaatgtata aatggaattc ttatcttaaa cattccaaat tttatatgag
    41701 gttgttccct tctagcgagt ctttccataa cttcactcca cctttcaata actcttataa
    41761 gtttacaact ttataattgt ttataaatta tcaatgtcat taaaattagt atataacatg
    41821 caaattatat aaacaataaa ttaaatctat aaaattgata gattttgtgc atatatagaa
    41881 agaccgcttc atttttagtt acttaaatca cttaatatta gttatttaaa tcacttgata
    41941 aaggtttttt ataaaattca atagttctat tccatgattc aaactgtcct tttgaatttc
    42001 cttctacagt tccacctgaa gaaaaaatcc ctctactcat actcatacat aaagtctcct
    42061 ttagaggcat aatatatgga attggtaacg gataacccat attattgtat gttaataatt
    42121 ctacgttata tggataatta ttttcttcaa agatttctaa tgcctctaaa caagcttcat
    42181 aactattcca tatgttatct tcttttccag caatcataag tatatctgct ttggaatttt
    42241 ctatttttat tctagcctct tttctattct ctgctctttt aagacttttc ttataagtac
    42301 tattaaatcc aaatggaata tcttttgata tatttttttt ctgttcttca aagaatatat
    42361 cattgtctac tttgatatat ggaaatggct ctcctttata tgaccatgat gaagtatcat
    42421 ttccactcat caatccatta agagcttgga aacaataaat gtgaggttgt gatactacaa
    42481 cctttttaat ttgttcatat cttgaagcaa gtaaaaaggc caattctcca ccctttgatg
    42541 tcccatgtac aaatatttct ttagtactgg taatttcatt ttcactaatc cactcaaata
    42601 ctttttcaaa atattctaat ggaacctctt ctaatttatc tggtaaccct tctaaagcaa
    42661 agtatgcaac tgttagtaca ttgaatcctc tagatgcaag tggagctgct aaaaggtcta
    42721 gagcgtctaa atttccatca gaaccaccta gcattaaaat tgttttatta ttagaatttt
    42781 gtttataaaa taattttcct ataaactcat cagatatgta ttttgtggta atatcttctg
    42841 atttaaatat tctctttaat tcaacttctt ttctttcaat agaagtttca aatatcatat
    42901 tcataattat tggtttatca atagatatat tttctgcaaa atttttacct tcagttttag
    42961 atttttccag cgaataaatt aaccccatac aattatcagc cttataagta ccttctatag
    43021 gttcaactaa atctaaatct acttctcctt tttcatttga acaaaacact ccataagatg
    43081 aaaattcttc tcccttacac catggaaatt ccattttcac acttatttta agtttagtat
    43141 ttggaattaa gtctgaaacc ataatcttaa gtctttcatc cacaaatgat tctacattgt
    43201 atacatttac tttcataatc tatttctcct tataataatt atttatgttt tatataaaac
    43261 agctttatct aatacaagat aaataaattt ttaaattaag acaccatatt gactatttca
    43321 ttttatcaac atatttcatc tttgtcaaca tattgtcttt tttagtaatt taaaattatg
    43381 gtctatatga aatccagttg atttttggct aattaaagat aggaatgcta ttaggtttag
    43441 aatatatata ttcaaaataa aaaaccactc atttaaagaa tggtttttaa tataaactat
    43501 ttgttattaa taaggatatt tttttattta aatttatata atcgaacatc taatatttaa
    43561 tgtttaataa aattatgcat ttgcaaaagt atatatggca ttaactcttg ctacatataa
    43621 attctcatca ctacatccaa tatatctagc atatttttca atcttttctt ttgcattttc
    43681 aacatcattt tcatcgctct ccattaactc atttatataa atatccatta atgacgcttt
    43741 atattctctt atatcatagc ctaaaaattc agtcaatctc tcaatttcct tactaaaatc
    43801 tccatcttct tttaccaaaa attcgtatac atcttcccta tatggattta aagttattat
    43861 ttgttttact actttgcatt cgtcagcctt cccaataaaa ccatctttat aattattaaa
    43921 taaggcatta gattttttta tagattcttc atcaatataa gaatgtaccg cagttatacg
    43981 attttctata agggcatcta caacagcata atgaatttta aaacaatcat tgtataaata
    44041 attgctcatt ttctttattg tcgattctat ttctaagaag ctcgcgtggc catttttttt
    44101 atttttattt ggtataataa aagttttatt caaattatta aaaagtctct taaaatctaa
    44161 atatttttca caataaacac ttttaaaggt atcgatatct atagttataa ttccaaaact
    44221 tactatagtc tcaattcctt tcttaaaaga ctcttctata tatttttctc ctaaacttaa
    44281 acctctatca gatatttgct ttagattttg aaaactattt aaacaatctc tttcaaagtt
    44341 tttagatatt tctaaagcat ataatttaaa gattttcctt atcttattat aattattttt
    44401 tgtatcatca aatctgataa tagctccatt tacatcaaaa gtaatcattg agttttaacc
    44461 tccccattag tttcatcaat aaaataaatt taatataaac
//
LOCUS       DAEMGG010000017        43481 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEMGG010000017 DAEMGG010000000
VERSION     DAEMGG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.017"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            73..702
                     /locus_tag="KPK24_000946"
     CDS             73..702
                     /locus_tag="KPK24_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2298653.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRSVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(816..2330)
                     /gene="glpK"
                     /locus_tag="KPK24_000947"
     CDS             complement(816..2330)
                     /gene="glpK"
                     /locus_tag="KPK24_000947"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG2298654.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2574..3116
                     /locus_tag="KPK24_000948"
     CDS             2574..3116
                     /locus_tag="KPK24_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2298655.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3130..5622
                     /locus_tag="KPK24_000949"
     CDS             3130..5622
                     /locus_tag="KPK24_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2298656.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            6043..6441
                     /locus_tag="KPK24_000950"
     CDS             6043..6441
                     /locus_tag="KPK24_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2298657.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(6658..6867)
                     /locus_tag="KPK24_000951"
     CDS             complement(6658..6867)
                     /locus_tag="KPK24_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298658.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(6899..7108)
                     /locus_tag="KPK24_000952"
     CDS             complement(6899..7108)
                     /locus_tag="KPK24_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298659.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            7407..7826
                     /locus_tag="KPK24_000953"
     CDS             7407..7826
                     /locus_tag="KPK24_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298660.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            8054..8509
                     /locus_tag="KPK24_000954"
     CDS             8054..8509
                     /locus_tag="KPK24_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298661.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(8716..8976)
                     /locus_tag="KPK24_000955"
     CDS             complement(8716..8976)
                     /locus_tag="KPK24_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298662.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(9239..9592)
                     /locus_tag="KPK24_000956"
     CDS             complement(9239..9592)
                     /locus_tag="KPK24_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298663.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANVEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(9609..14930)
                     /locus_tag="KPK24_000957"
     CDS             complement(9609..14930)
                     /locus_tag="KPK24_000957"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298664.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNALISQYT
                     EISLSNVTDAYSSYNATFIVVDEKKVYATGINTNYLLGFSTSDGSNVNLGLLSDWYYI
                     NISGSSYSRVSCTNNITKINNIIIYEYVTVFCTNIGSFLTGYHGTSWTKPTDSSYRVQ
                     YQGISYAGYLDSYIYNYYPTRCTQSSSSTTFAYLYNGESSSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNIFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYLCNDVSIIVKNDNTCYVCGLVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTRDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(14947..15930)
                     /locus_tag="KPK24_000958"
     CDS             complement(14947..15930)
                     /locus_tag="KPK24_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2298665.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(15931..16629)
                     /locus_tag="KPK24_000959"
     CDS             complement(15931..16629)
                     /locus_tag="KPK24_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG2298666.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(16626..17678)
                     /locus_tag="KPK24_000960"
     CDS             complement(16626..17678)
                     /locus_tag="KPK24_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG2298667.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(17671..18099)
                     /locus_tag="KPK24_000961"
     CDS             complement(17671..18099)
                     /locus_tag="KPK24_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG2298668.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(18099..18425)
                     /locus_tag="KPK24_000962"
     CDS             complement(18099..18425)
                     /locus_tag="KPK24_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG2298669.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(18441..19970)
                     /locus_tag="KPK24_000963"
     CDS             complement(18441..19970)
                     /locus_tag="KPK24_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2298670.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGIIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(20252..20674)
                     /locus_tag="KPK24_000964"
     CDS             complement(20252..20674)
                     /locus_tag="KPK24_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298671.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(20674..23127)
                     /locus_tag="KPK24_000965"
     CDS             complement(20674..23127)
                     /locus_tag="KPK24_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298672.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(23127..23294)
                     /locus_tag="KPK24_000966"
     CDS             complement(23127..23294)
                     /locus_tag="KPK24_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298673.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(23321..23767)
                     /locus_tag="KPK24_000967"
     CDS             complement(23321..23767)
                     /locus_tag="KPK24_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2298674.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(23881..24309)
                     /locus_tag="KPK24_000968"
     CDS             complement(23881..24309)
                     /locus_tag="KPK24_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG2298675.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(24323..25387)
                     /locus_tag="KPK24_000969"
     CDS             complement(24323..25387)
                     /locus_tag="KPK24_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG2298676.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(25392..25835)
                     /locus_tag="KPK24_000970"
     CDS             complement(25392..25835)
                     /locus_tag="KPK24_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298677.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMKEINLNLGE"
     gene            complement(26084..26524)
                     /locus_tag="KPK24_000971"
     CDS             complement(26084..26524)
                     /locus_tag="KPK24_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298678.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(26629..26826)
                     /locus_tag="KPK24_000972"
     CDS             complement(26629..26826)
                     /locus_tag="KPK24_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298679.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            26995..27315
                     /locus_tag="KPK24_000973"
     CDS             26995..27315
                     /locus_tag="KPK24_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2298680.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            27397..27903
                     /locus_tag="KPK24_000974"
     CDS             27397..27903
                     /locus_tag="KPK24_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298681.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            28604..29359
                     /locus_tag="KPK24_000975"
     CDS             28604..29359
                     /locus_tag="KPK24_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2298682.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(29502..29930)
                     /locus_tag="KPK24_000976"
     CDS             complement(29502..29930)
                     /locus_tag="KPK24_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG2298683.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(30351..30551)
                     /locus_tag="KPK24_000977"
     CDS             complement(30351..30551)
                     /locus_tag="KPK24_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2298684.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(30734..31903)
                     /locus_tag="KPK24_000978"
     CDS             complement(30734..31903)
                     /locus_tag="KPK24_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG2298685.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            32139..32960
                     /locus_tag="KPK24_000979"
     CDS             32139..32960
                     /locus_tag="KPK24_000979"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG2298686.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRHEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(33036..34424)
                     /locus_tag="KPK24_000980"
     CDS             complement(33036..34424)
                     /locus_tag="KPK24_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG2298687.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGSLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(34559..35317)
                     /locus_tag="KPK24_000981"
     CDS             complement(34559..35317)
                     /locus_tag="KPK24_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG2298688.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFASSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(35319..36035)
                     /locus_tag="KPK24_000982"
     CDS             complement(35319..36035)
                     /locus_tag="KPK24_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG2298689.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(36037..36744)
                     /locus_tag="KPK24_000983"
     CDS             complement(36037..36744)
                     /locus_tag="KPK24_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2298690.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEKLFMKVVAENRKRGQ"
     gene            complement(36860..37396)
                     /locus_tag="KPK24_000984"
     CDS             complement(36860..37396)
                     /locus_tag="KPK24_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2298691.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(37498..38568)
                     /locus_tag="KPK24_000985"
     CDS             complement(37498..38568)
                     /locus_tag="KPK24_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG2298692.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(38561..39619)
                     /locus_tag="KPK24_000986"
     CDS             complement(38561..39619)
                     /locus_tag="KPK24_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG2298693.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(39606..39938)
                     /locus_tag="KPK24_000987"
     CDS             complement(39606..39938)
                     /locus_tag="KPK24_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG2298694.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            40137..41258
                     /locus_tag="KPK24_000988"
     CDS             40137..41258
                     /locus_tag="KPK24_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG2298695.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(41386..43284)
                     /locus_tag="KPK24_000989"
     CDS             complement(41386..43284)
                     /locus_tag="KPK24_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG2298696.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT        14403 a         7195 c         4838 g        17045 t
ORIGIN      
        1 ctataaatta tacaatagta ttgttaagat tatatatttt tttaacataa attttgtatt
       61 gggggaaaaa caatggataa aaactatacg atgccaatat accaaaaaat agcgttggat
      121 attgcaaata aaatatacac gggtgaaata caagaggatt cagtactatt tggacgctct
      181 gttttggctg gtaaatacaa tgtatctcca gaaacgataa gaagatctgt caaaatttta
      241 gaggacattg gcgttgttaa aagcataaag ggtaaaggtg ttattgtact atctccagac
      301 aaagcttctt cttttataaa aaaatataga gatattacaa atatatcatc atataaatca
      361 acactttata atttaataga tactaaatct aatttagaaa atgaaatatt agatactata
      421 aataaaattt tagactattc taacagactt gaaataatca atcctttagt gcccgttcag
      481 ttcaccataa attctaattg caaatatatt ggtcaaacag ctgcacaaac taaattttgg
      541 caaaatacag gtgctacaat agttgcaatt aaaagaggtg aagaacttat aatatctcct
      601 ggaccatata ttgaattttt agaaggagac attctcttag tagttggtga ccaacacatt
      661 tacaattcta taccaatgtt tttatatgaa aacgaaaaat aaattatata atttagtatg
      721 attatataaa aatgcaaaat taacaacaaa aaaagagctt catattttac ataaagctct
      781 ttttaaattc aaataaattt aactttgatt aatttttaaa tatccaattc atcttctttt
      841 tcccagctta aagctctcga tacagctttt ttccatcctt tatataattt acatctcttc
      901 tcttcactca tatttggatt aaactctttt tctactgacc actttgaagt aatttcattt
      961 cttccaatat agaatccaac agacaaccct gctaaataag cagctcctaa agctgtagtt
     1021 tctactattt tgggtctatc tatatctaca tttaatatat ctgattgaaa ttgcattaaa
     1081 aaattattat tacttgctcc accatcaact tttaatgatt ttagttttaa ttttgaatcg
     1141 ttttgcattg cttctaaaac atcctttgtc tgataagcaa ttgattccaa tgtggctctt
     1201 actatatgtt ctttttttgc tcctcttgta agtcctaaaa ttgaacccct agcatacata
     1261 tcccaatatg gagctccaag acctgtaaat gctggcacta catatacacc attagtatcc
     1321 tctaccctat ttgcatacat ttcactatct tttgcagatt caattatctt taattcatct
     1381 ctaagccatt gaacagatgc tcctccaata aatatacttc cttctaatgc atatgttact
     1441 tttccatcaa ctccccaagc aattgttgta agtaacccat gcttagattt aactatgtta
     1501 ctgcctgtat tcataagtaa gaaacaaccc gttccataag tattttttgc agtaccttct
     1561 tcaacacaat tttgaccaaa taatgctgct tgttggtctc cagcacatcc cgcaataggt
     1621 attttagctc ctgataacat gcgctcatct gtatgaccat aaacatagct agaaggtttt
     1681 actaatggca acatgcttat tggtatgtcc aattctcgta atatatcctc atcccattct
     1741 aaatcattta tattgtacat cattgttcta gatgcatttg agtaatctgt tacatgtact
     1801 tttccacgag ttaaattcca tattaaccat gtatctatag ttccaaataa taactctcct
     1861 tgttctgctt tttccctagc tccttctaca ttatctaata tccattttac ttttgttgct
     1921 gaaaagtatg catctattaa aagacctgtt ttttctttta ttgattcttc caatccttta
     1981 tttttaagtt catcacatat ttctgacgtt ctcctacact gccatactat tgcgttataa
     2041 ataggtttac cagtatacct actccaaaca accgtagtct ctctttgatt agttatccct
     2101 attgaagcaa tttcttctgg tcttatacct gctgtttcta taacttctct cattacccca
     2161 ctttgtgacc cccatatttc catagggtca tgctctaccc atccagcttt tggataaatt
     2221 tgattgaact ctttttgtgc aattttcact atttcgcctt ttttattaaa tagtatagct
     2281 cttgaactgg ttgttccttg gtctaatgcc attacatact tcttttccat caccatattc
     2341 tccttatcat ataatattta atttattaaa aagaaaaatt taatatcaca tatttcaata
     2401 aataatcata cttaagtaca aatttaatat aatagatatt tagtaagaag acacttattt
     2461 tctatcatat ctatataata atattataca ttatagctta catattgatt aataaatttt
     2521 aaaggagtat aataaacagt gttcataata attttaataa aagaggtggt aatatgccaa
     2581 agattttaga aaatgtaaaa gaagatatat taaaagtgtc tcgggatatg attttagaag
     2641 aagattactc gaatattagc ataaggaaaa tagctcaaag gtgtggtata agtgcaggta
     2701 cagtgtataa ttactttaat tcaaaacaag aaataataga atatatcaca aaatctgaat
     2761 gggatttatt gataaggcga attgaatact ccaataaaaa cactgaagat tacataaaaa
     2821 aactaaacat tatttttact gaaataagaa attttataaa caaagttcat aatatccaat
     2881 acaatgactt tttaaacact tttgaaacag agagattttt tgaaatgaaa aaacacaaag
     2941 acgactttca tgaacagctt tcaaataaag tatatgaagc tttagaaaaa gaaagttttt
     3001 ttaataatga caaattagta tgcaatatca taataaaaat gtttttttca tattcaacat
     3061 atcttcatat agaatttgaa gatttaaaac cctatattga aaaattaata cattaaggtg
     3121 gtgtttacct tgaatcataa tactagtatt aagaacaagg acagtaatcc aaaaatattt
     3181 ttagtactta cactttatct attaggtata tttatgggag ctattgatac tggaatagta
     3241 tcaccagcaa gaacgattat acaaaatagc cttggagtaa atgaaaaaac gggaatttgg
     3301 atgattacca tatacacatt aacatacgct tctgtaattc ctatctctgg aaaattagca
     3361 gataaaatag gaagaaaata tgtgtatcta gttagtatat ttctatttgg tagtggttca
     3421 ttaatatgtg gattatcatc actattttcc aatttctata ttttattaat tggtcgtgtt
     3481 attcaagcaa ttggtggtgg tggaatcatg ccaattgcta cagcagaatt tggaactacc
     3541 ttcccagaaa acaaaagagg catggcttta ggtttagttg gggcaacata tggtattgcc
     3601 aatattttag gttcaagcat tggtagcaca attctaagta tatttggaac tcaaaattgg
     3661 aagtggctat tttttgtaaa tcttccaatt tgtttaatca tacttattgg tggagttttc
     3721 tgtattaaaa acaataagtc tgaatctaaa gaaaaaatag ataagcttgg tacattgatg
     3781 ttgatatgta taatcgtatc actattatat ggtcttatga atatagactt ttttaatttt
     3841 aaaaattcta tacaagatat atcagtttat ccatatttat tgacatttat tgtacttata
     3901 cctattttca tatttattga aaatagagct aaagacccta ttttaagctt tgaatatttt
     3961 ttaaatccga gaatattgat agtacttatt ttatctttaa ttgttggtat tggaatgatg
     4021 ggtatggtat tcgttccaca gtatgctgaa aatgcattaa agataaatgc tggttctggt
     4081 ggatattttg tagctatatt gggattattt gcaggtattg ctgctccttt aagcggaaga
     4141 cttattgaca agtttggggc aaaaaaaata ctattactag gattttctgt atctatgata
     4201 ggctctcttt acctagtttt aattgctcta aaaaccaata ctgtatttag tgtatgtgta
     4261 agtttaatat ttatgggatt aggaatggga tttactatgg gaactcctct aaactatatg
     4321 atgttatcaa atacaagatt agaagaatct aattcagcac ttgcaacatt atccttgata
     4381 cgctcaattg gtacttcaat ttctccagct ataatgattg gatttatagc acatgcagga
     4441 ttatctgtgc aagataatat tatggactta gttggaaagc cctcaactcc taaaatagtt
     4501 caacttgaag aacttgaaaa catgatagat gacttaaaat cagaccctga aatggcaaaa
     4561 aatttaaaaa atgtctctat cccaaatatg aatgatactt caaatataaa tatggacatg
     4621 acaggtggca aacttccaaa atccttactt aataaagttc agtcagcaga tacaaccaac
     4681 attaccaata taacaaaaga aatatcaaca acaatgtttg atgaaaaagt tccatctata
     4741 attactaaaa tacagaaaaa tgtacaaaaa ggaatagatg gaacacaaag tggaataaac
     4801 ggtattgaag atggtgaaaa taaactaaat agtggtatta aaggagttca aactggtatt
     4861 gacaatatgg taaaagctag agctggtatt aatcaaggaa tagatggtgt aaaaaaaggt
     4921 attgctggtg ttgacaaagg tataaaaggt atggaacaag gtcttgaaaa acaagatgag
     4981 gccatcaagg aacttacaac tgcttataat aaaataccta ctacagaaaa aactcctact
     5041 gataacgaaa ataactcaaa cgaacaaaac cagagtaata ccagtaattc tgaatcagat
     5101 gacactaaga atgattctac agaaaataag aataacaatt ccagcgagaa taaaccaaat
     5161 gtaaatgaac aaaaagaaag tttaaatgct caaatacagc aactaaaaaa gacaagagat
     5221 gacttaaata caaagttaca aaaaaataaa actcaaaaga acgaattaag taaaaaacta
     5281 agatcaatgg aatctcaaaa gaaagagcta caaagcaagt taaataattc tataagccaa
     5341 aagaaagaca tggaaaaaag ccttgataca atgcaacagc aaaaaaaatc actccaagca
     5401 gttttagcaa agacacaaga ggtaaaaact gaaataccaa aggcttttga taaatcaaaa
     5461 ttggattata taaattccat agaaaataat agaacaaaaa tagaaaacac actacaatct
     5521 actctaaact caggatttaa acagatgtat ataacagtat tctgtgtaaa tctactagca
     5581 tttataatat tattgttcta taaagaaaat aaaactagat aaaagagtgt tatagtatta
     5641 tattgtaaat ttatcttatt cttaaatatt ttattctaaa gaataaattc ataaaatttt
     5701 tgtaattgat taatataaat tacaatataa tactattttt ctcttaatct ttttctcttt
     5761 tacagacaat caaaaagcaa tcttagttta tcgaaataaa tttatacaat ctattcatac
     5821 acccctttac atttatataa caattaaacc tattgttata tatacaaatc aaaataaacc
     5881 atatattttc aactataata tgttaaaatc atattatgag catattaatt taagcaaaat
     5941 gtcatttaga ctttaattta gtagttttat attataaatt tatatatttt taattttaaa
     6001 tgtcatttag acattactca taaaaagaaa ggagtgtatt ttgtggatac tttaggaaaa
     6061 agaattgctt atttgagaaa ctccaaaaaa cttactcagc gcaaacttat ggatatttta
     6121 aaatttgaaa acttaggcaa gtatgaaaca ggagatagaa aacctaattg tgatatacta
     6181 atgtcaatag ctgattattt taatgttacg actgactggc ttctctatgg taaagagaag
     6241 gtaaatgtca atagtagtgt aaaagaagat aaagaagatt acttacatgt tactaatgat
     6301 gaaatgacga tattaaatct atacagacaa ctaaacgaac gtgataaaat aaaaatagaa
     6361 ggaatacttg aacttaagat atctgaatat aaagacttaa aaaagcattc ctctaataat
     6421 aatgaagata aaatggttta aacatacata atttgatacg attttacatc atctattaac
     6481 cttctttttt tgtagtatat tactaaaata tggatattgg attatttaaa aacaagtata
     6541 gtctaatttc aagcacattt ataatattta gattatctta tccatatttt agtttaagta
     6601 ctttgaaaaa tataatacat ctctatgtat aacttatatt cctaagaaat gcattactca
     6661 tgtttttaat tcttccaaca tctttctata cacaattgta catccttcat aattcccctc
     6721 tttaagaagg attgaacatg tactagaata tttagttaaa gtatttaatc tttccatatt
     6781 taagcttttt atattactga gtgctgtaat aaaatcatca attgctctac aacactctat
     6841 aaccccatct gaatccaatt tacacacaag aatattcacc tactttatat actatttatt
     6901 aatcatcttc tgaatattct atattaagta attgataaat tgatgattta tattttttac
     6961 ctgttcttct tccatttaaa atagctgaca aatagttttc attaacacct attttcttcg
     7021 ctaattccct ttgagtcata tttaattcaa taagtctttg cttgacttgc actcccaaaa
     7081 aaggtatcca tttttttcga gcatccattt atcaacttcc tttctaagcg atttatattt
     7141 acttttatat tattttagct tacgcacccc ctctatttag tatatatttt taatatgtac
     7201 acatattaag tatataaatt tatttaatac taaataagat tttttgatta ctttgtgcta
     7261 aaattaggat gtaaattcgt atcattaagt taattataaa gtcattttga ccttaagtca
     7321 atggttttta tgtcattttg acctttaatt tgaaactatt tacatcttaa aaagtctttt
     7381 tgacattaat tgaatggaga gtgtttatgg atactttagg agaaagaatt gtttatttga
     7441 gaaaagcaaa aaaccttaaa caatatgaac tagaagaaat gttgggctgt gacaacttaa
     7501 gtaaatttga gagaaacatt agaaaaccaa attatgaaat attaaaatct atagctgaga
     7561 tatttaatgt ttcagtagac tggctattaa atggagataa tctatcacat aaatcagatt
     7621 taatttgcga ttcctcctca aactatccac ttaatagcat aaactctaat gaaataaagc
     7681 ttttaaacaa ttttagaaaa ttaaatgatt atgataaagc taaaatagaa ggtatgattg
     7741 aattaaaact tcatgaatat gaaaaagaga aggatttagg aaaaattgag tataacaaaa
     7801 ataaagatga gaaaatagat aaataatttt tatgttgctt ttgatacgat tttacatcat
     7861 ttctttgttc taatattttt atttcattta ttttcttttt tataatttta tgataaaata
     7921 attagtatat tttgctttac ttgaatgtaa ttatttatac aaaagtcaaa cataatataa
     7981 atgtagtaaa tttttagttt caagtaatat ctcgttatta taactaaata tttattaaaa
     8041 ttaggaggat aaaatgggct taagagacaa attcgcgcaa tcatttgcta gatcaaaaac
     8101 tatgagtggc cctgagaaaa aggctaatga aataatgggt aaattgcttc ttaagaaagc
     8161 tatattacct atcgttttaa tgttcgtgat aataatcgct ggagctatgc ttaaaatcaa
     8221 ttcttgggtt acacttggca taaatttagt tattgctgtt ggtgctttct tctacataag
     8281 aaattctagt aaaaagtatc agaactttaa accttatgtt ggcaatttga ttagtttaga
     8341 gaaaaaaggt aaaaaagagt acgttgctat tataaaacaa ggtaagttac cagttaagtt
     8401 acaaatagct tatggtggtg aagaccttga acatgttaag aaaaatcaaa tggttcaaat
     8461 aagttataat ccagatgcta aaatagctat tttggttaat agacaataat ttaataaaaa
     8521 aaaggagttg tcttaaaatt gattttttct aatcaaattt aagctcaact ccttttttaa
     8581 tttttatatg ttatttttat tacttttttt aattaatatt ttaaatatta aatccttctc
     8641 aataaaatag tgaactatct acaatactac tttatctttt cctaacaata tataatctgg
     8701 tgctactctt tcatgttata caccattttg taacatacct tttatatcag taacatcttc
     8761 cttaatttcc tctacatctg ttttcattat acccatttga acaagtatat ttttgtttat
     8821 ttcttgttgt tgtgtagata attctataaa attttctaca gtttttttat acatgtctct
     8881 atcttctttt ttctcctgca tagtattttt aaataataga gcacatagta tacctattgc
     8941 ccctagacta ctcaattcgg ttattaattt atccattata gcctcctatt gtctaaattt
     9001 tttatactta tagctttaat ttttgttgta ttcttacatc atgcaatatt atattttcag
     9061 gattatcaat ttaatcttct gtactttaaa ttgtaggtgt aatttataga ttgaataaat
     9121 aataaatata tatacaagtt ctaatgtaac aaaaaataag tacaaggctt acttattaaa
     9181 gttaagacct tgtactttac ataaaaaaaa taactaatac atagttattc taaatttact
     9241 attcaactgg tatttctaat tctttacatc taattctaac ttgctctcta attactgctg
     9301 gtacttcttc tattgttttt ttgcctctta aaattaaagc tacatatact tctaccattc
     9361 cactctcacc tccagatttt cttacactaa aagtgcttat attacttgct tcattgacag
     9421 aactttgatt taacaacatc tcatatattt ctacatttgc tataaaatta tttgttatat
     9481 catcatcttg ctttacttga cctatactta tgtctgaaat gtctttatac tgcatttcta
     9541 cttttctacc ttgtttgatt tgttctattt tatttttatc gaatttaaac aattttcaca
     9601 cctccatttt aagcttgtgg ctcgttgttt tcagcatcat atctaaaaat catagatgga
     9661 cgttttatag ctgtccattt atctcctttt tctactatga ttttaggctg agcacttttt
     9721 atgttggctt cttctaattc atagatttct tggtaacttg agtctgattt tttgatttca
     9781 actagtttca tgtctttgaa taagtcagat ttatttattt ttacttgaac tttagtttta
     9841 tttattaatt gatatatagg attgttttcc atcatatcat cctttaatcc ctcatttaat
     9901 gttactatta agtcagtgtt attttcagta atgtttatga ttgaatatac tttattgttt
     9961 atcattaaaa tagaatcctt ttttacttta agccctgttt ctgatttaaa tataaacata
    10021 cttgaatata aatcgcctgt aggactactg gctttaatca gtatcttatt ttctcctaat
    10081 actattttat tttgtggtat ctcaaatata atgctattta tttccaaatt agtcttagta
    10141 gatattaatt catcatttat aaacatttca agtttttcta taacattatt agggtcttgt
    10201 actacaactc tcacttgaga tattgatttt tcaactattt ctatagatgc tttacttgta
    10261 tctacttttg ttatcatgta tattgtatca tctgaaagct ctatatctaa aatatcattt
    10321 agatgttcag gaattggtat tttagtaaat tcatataaat cagtaattga tatagatatt
    10381 ttatacatag atttattttt accagttatc catagactat tatcattttt aattactagt
    10441 atgtggtcat cattatgtga cttaatttct ttaacattag ttaatacttt agtaaaagtt
    10501 cttattatct tatctttttc actttctcct agtccaaata atccatttgt attgtaccct
    10561 tgagcgtaca actctccctc attagttaaa acaagtgtca ttgagttata tatacttata
    10621 tctttaacag aaggtaatcc agtaaccttt tgtaactctc ttacatcatt attgtggcca
    10681 actcccaatt ggtactcagt attctttcca tatgaccata gagtattatc tgtcttaata
    10741 atataggagt agttatctcc acaagcaact tttttgacat ctgatatatt tacttcagta
    10801 agagttaata attctatatc ttcaagggtt tgattaattt ctttatatga attacttcct
    10861 attccatata acttgccatc cttagcaaca gccaatgaat gtgtttttga tatatcaata
    10921 tcagatacct caaatggcaa tattattgaa gtatacttaa aattatcttt aaatatgttt
    10981 ttataattta tatacttatt atttgttttt atatacttat cattaaacat tactagcttt
    11041 tcatttttct tattaaaaac tattttatct gatgtgttat ctgttttaaa atatgaatgt
    11101 agggaacctt caagagcttc atttttatca ttatcatatc tatggtgaat acctaaatga
    11161 ttatatctat tatctcttgt tgaagaataa aattttccat atatatcttt aataaatata
    11221 gtattgtaac tagaaaatac agtatcaaca tctttcttta tagatacttt ataatatgga
    11281 actgacatat ccctataaga ttcatcagat ataccataag tatctctacc tgatgatatc
    11341 aaattgttat tattatctat ataaagtgta ttattagctg ttacaaaaac atctttaaca
    11401 tcttccacac tatttgtatt attattattt gctatacttc catatccact aagacctcca
    11461 acaaatagtt tattgtcatt tgttaaaatg gctgaagtat atccattaac tactattttt
    11521 tttgcattta taatattatt aattactcgt atccccttta tatcattagt ctctgttgaa
    11581 aagaataccc tctctttttt ccctgtagta taaatcatat tgtcatttgt tataaaaaat
    11641 gtagcttcac ttcctgctgt cacaacagat tttacattct caatatttat ttttgtaaat
    11701 gtacttatac ttccttcagt aaacccaaaa tagccattta caagtccaca tacatagcat
    11761 gtattatcat ttttaacaat gattgataca tcattacata gatatacatc tctaacacca
    11821 tttataggta tacgtgtaaa tatattccta cttgtagtat cacccaatcc taactgtcct
    11881 tttccattat tacctgctcc atataaaaga ccatcattag ttaataatag agatgtgttt
    11941 ccatatacag ctacatattt tacattttca acatttattt tagtaaatat atttctattt
    12001 acattatctc ctattcctaa ctgaccagaa ttatttaaac caacagcata acaggtatta
    12061 tcatttttta tcgcaaatgc atgtttacta ttgtcagtaa atctatttac atttatagat
    12121 tttatattat caatatttat tttagtaaat acctttctat ttatctcatc tcccactcct
    12181 aattgaccta catcatttaa accaacactg taaagcatat tattattagt taataaatat
    12241 gtagactttg aatggctata tatttcttgt atattagaag atatttcact actgctattc
    12301 tttttattgt aatttatctt attatttaaa tttaaactac tattaactga tataccatgc
    12361 gtacccttag agttatatcc acatataaat aagctcttat cagttaatat tgcaaatcta
    12421 gttttatctc tacttaaatc ataaggaggt ataaatatat tttttatatt tttcattacc
    12481 tttttataat tagtggcatt atatccatct tgtgtatctg aaacatctag ttcttttgca
    12541 ctttcagata tattcccaaa accatatagc gtgttagaag ataaatacaa tagagcttta
    12601 tataaaaata tggctttatc agaggtaata ggacctgaat ttatattaga tgctgcaata
    12661 ttattagatg attgattgtt aagataattc cttccatatt gatgtatata agatgaacct
    12721 ccagaaataa gtaaattatc tggattgaca ttttttaaat tacttgacga ttccccatta
    12781 tataaataag caaaagttgt agaagatgat gattgtgtac atcttgtagg ataataatta
    12841 tatatataag aatcaagata tcctgcatat gaaattccct gatattgaac tctatagctt
    12901 gaatcagttg gttttgtcca tgaagtacca tggtatccag ttagaaaaga tccaatgttt
    12961 gtacaaaata cagttacata ctcatatatg ataatattat taattttagt aatattattc
    13021 gtgcatgaaa ctctactata acttgaccct gatatattta tataatacca atcacttaat
    13081 aaacctagat ttacattaga tccatcagaa gtactaaaac ctaacaggta atttgtattt
    13141 ataccagttg catatacctt tttttcatca actacaataa atgttgcatt atatgaacta
    13201 taagcatcag ttacattact aaggctaatc tcagtatatt gacttattaa tgcattacta
    13261 agtccattaa cataacttga actctttcca gaagtgtata tttgcttatt attagttaca
    13321 attaatgaaa accagtttga taaatcagta tttataacaa ctttttttac attatctata
    13381 gatattttct taaatacatc tgaattagaa ggaagaacac ctgctattga gttactattt
    13441 ggtccagaat aataacaagt atcatcattt ttaatgtaga atattcctcc agaaatattt
    13501 tttgatgcta ctatagactt tacattagag atacttaatt ttgtcaactc atttaattga
    13561 cttggtaagt cagaacccat tcctttgtat ctattatttc ctagaccata tacatctcca
    13621 ttccctaaaa taaccagagt acaataagga ctaaaaacga catctcttac agaatgtata
    13681 tctagtggaa gttcaatatc ctttatttcc atttcttctt ttaataaagt taatttatcc
    13741 tcagatttaa ttaaatttat ataattacta taactatatt tttcactatc cattttctta
    13801 aattctacaa tgtgcatatc atcaaaaaac gaacctaatt gtccataagt attatctcct
    13861 gtacaatatg aatcattatt tgaatcaatt agtatgctat gtgataaacc aatgaaaata
    13921 ttcttagcta caatattttt taaatctata aatgaattat ctatattctc agagtaattt
    13981 gctataccta actgatatat atcattattt ccagcaacct ttaaagtacc tttgttatct
    14041 ataagcatag tgttaccatg accacatgca atagttttta tatttacttc ttctatctta
    14101 gtaaatagtg atgattgaag tgcatttcca aaacattttt cctttccaca accatatata
    14161 tcattattat acgtcattaa cacagtgtgt tcagaaccac aagctatagt cttaacatca
    14221 ttaatagaaa ctaaactaaa taaatctcta ttattcttat ctcctaaacc tagttgacct
    14281 tgattattaa aaccagtaga atagcattta ttgtcattct ttattataaa agctgattta
    14341 ttatatgaaa atacagcctt tacattgtca acatctactt ttgtaaatgt atttacattt
    14401 gcagatgctc cattagttcc tagacctaat tgaccataca aattatttcc agcaacatat
    14461 aaagttccat cattgtataa aagatatgtt gattgaagtc cacatgcaat atcctttacg
    14521 ccttttattt ctggaacctt ctcaaatgta gaaactgttt tccctacttt ttctaatccc
    14581 aatcccattt gaccatctgt atttatacct gcgacaagta attctccatc tgaagtaagt
    14641 ataactgcat gagattctcc acatgcaact tttttaatat tggtagatat atttggtatt
    14701 ttcgtaaata attttcttgt tgtattgtct tctcccaatc caagttgaaa gttactatta
    14761 tctccacatc catacaagat attattattt tttagaccaa atgtatagtt acaacatcca
    14821 gtaaaaaaat taataaagtc ataattatca tacccttcat caaattcatc aactaccata
    14881 tttttatcgc caaagaaatt acctctttga agtagtttag ttcttttcat tataaaaacc
    14941 tcctaattat cttatttcat acgaatattt atttaaagtt atatctgtct gaccaattga
    15001 atatgcccta ttagattttg tttcaacaaa gttattatcc atttcatgta tatatctaac
    15061 tctatttgca ttaaatggta gcaccacatt cttaaatgta acactaccaa gttctccagt
    15121 tatacacttt ctatttaaat ctaacttaag gccttctata gctattatgt ttttagcatt
    15181 agttaaggta tcataccaat atccagcttc atctacatct atttctgact ctaattctaa
    15241 gtatattaag atatctaata ctttttcaga taatttattg taactatttt ttaatacaga
    15301 tagttcagtt tgtatttgac ctcctattac atcatcaata gatatatttt taattttttc
    15361 tactacctca tttatagctc ctgataagtt tttagcttct gtagataaga gttcttcgcc
    15421 aatttttact tttatttctt ttatattttt atcaaactta tcatctaaat cttgaatatc
    15481 cttttgagtt acaaaaacca acgtcgggtc tacttctaat gtaacatttg aagtattgga
    15541 caattgtaaa atcattttta caaccaaatc tttaatactg ccatcttcag cacgtggctt
    15601 atacgtctct ggatacttac ctatagctat tatattattc tcattatcca gaacagcagc
    15661 ttctcttatc ataaatccac caactgttcc tggtaaaata gtctctataa ctatccaatt
    15721 aggatttttt tcatcaattc ttagagaatt tatatttcct tcccaaacta cattctttaa
    15781 agcagtttgt tcctctgttg gctcataata ttctccatta ctatctccaa cttgaaactt
    15841 ggttaatcca acaagctctc ctagtgcagt tgcatttgct attttttctc taccaatttt
    15901 ggttaatatt atataaaatt tttcttccaa ttataccacc tccgatttag gataaactac
    15961 tacattttct acacctgtat catttgcaat agctatatta atcttacaat tattttcaat
    16021 gcatttaatg cttaaaggat aaacctttat ttcttctcta cttatcattc cagctccaca
    16081 gtttacaaca gcactacatt ttataattgg ttcaaagtat ggatgaactt ttacctcttc
    16141 actacatacc atacctccac cacaatagac catactttta tctagtatta ttggttctaa
    16201 atagtgtaat aagtgagcag gttttactct ttcaatcata tcgctacaat ctaaaagagt
    16261 tttatagtca cagttatttg ctataaaact caatacgaat gtaaattcat cactataaac
    16321 ttttatatct gtttctgatt ttgtatatgc ctcacatata ctttttataa cttcaatagt
    16381 actagtacct ctgcttctca ttttagcttt tatattgcta cgtcttgtgt caaaatcaag
    16441 ttcttttttt tcaatgcaga gtatgtcttc ccacatatct aatccccatg ttgcagtgtc
    16501 aacaaataat tggttaaagg tatcatcaat atcttcttta agaatattta attctgtatc
    16561 gtatgcacct tgtatctcct ctacaatgta atttctatca aatgatggta gtttatcaat
    16621 tagcttcatt gattttccac ctcactaaaa ttaacagttg ttacactagg tattttatct
    16681 tcattaatag ttatattatc tgtacttcca tttataagta ggttacttaa atcgtgtaca
    16741 ccagtagtat ttataagtat tcccattact tttgtataga ttatctctcc tctaatatct
    16801 ctaaagtatg tatttatact ttctaggaaa gattctttta cattgtctaa tgtatatcca
    16861 tcttctagtt tcattactgc acttatactt atttctattg gtaatggtgt cacaactgtt
    16921 atagttggtc caataggctt ctcttcatct atatgttgct gacacctttc aattgtttct
    16981 gtatcaacag cttgattatt tttcccaaat atcaagacct taactgttcc cggaccatcc
    17041 catcttggat aaaccttaac attatagact ccatctactt ccaaagccca ttcttcatag
    17101 tgagctttat ttccacttgt agcttgattt ctttggattt tataaaatct ttcttttagt
    17161 tcttcatctg tctctatatc tgtaccacct tcaaaacttc ttgtgttagt aatctttgtt
    17221 actccagata tattatcaac tagtttaaat tcacaatttg taggtaaatt atatttctta
    17281 ccaacttcca gagcttgaac tgggctattg tcaccttctt cactaccaat agttacatct
    17341 tttattacta caaatagtaa atctctatat gatattattg tgccattatt tattacagta
    17401 cctttctctc caataaattc aacttctcca tttgactctg tacctaactt tctatataca
    17461 ccaaattcat tgactctttt atcaagaaac tggttatatg tgtcttgtat aaaagccatt
    17521 ttatgcatct ttgaaagttc tagatatatc ttcgaaagtt ctagattatt tccagatacc
    17581 atgttgttta gaaaagaacc ttctccttta taaatatcaa gattaatatt ttcaagagtt
    17641 ctatttttta ttacttcata tgtctggtca ctatacattt atttccacct ccccatagat
    17701 agtggatact tttacatttg cactcaatat gtctctgtta aagttagcac tttttacgtt
    17761 tacttctaat atatatggat ttattagaag ggcctctttt atgaatctac tagcttcact
    17821 ttctgtaaga ccttttgtat atttttgtcc tattagttct gaaagctctg tcccatattc
    17881 taaagagtat atctcatgtt catatctatt tgtctttata cacttgtata tccaaacttt
    17941 taaggcttct tttttttcaa ttattttaaa gtcacctttt tctaaaatag gttcatcttt
    18001 ttcaaaatcc catgccactt cacgaaagat tggcaattct tctgttttag gtaagatata
    18061 atcctctggg acacctataa aaggaaatat tgtactcatt ataagctcac caacttactt
    18121 acaacagcaa atttttcgcc tattctaaac attattacca tatccccaga ttcaaagtta
    18181 tctataaaag gattttttac ttcatgctgg tgttcttgat ttgtttctgt gtcaaatgtt
    18241 tctagctgtc tatcaatcat ccaactatct atcaaaatat cttctttttc taatatgatg
    18301 ttatttgtct ctatttttaa atctggtaat ttacttttaa tttttccaat aaaaaaagaa
    18361 ggttgattat gatactttcc ttcttctctt attattccta taaattcatt tattggatta
    18421 gccattatat caccatcctt ttataatact ctcctagctg tgttaaaatc tcttctacta
    18481 cttaacttac ttattttaac tacatcacct ttttgtggag catgtaaaaa ttctccatta
    18541 cctatatata atccaacatg gctgactgga ttatgaaaaa atactaaatc tcctgcttga
    18601 agattttttt gttctacttt cttgcctttt ttagattgtt gatttgacgt tcttggcaaa
    18661 ctaacattaa cttttttaaa acaatagacc attaaaccag aacagtcaaa actacttggt
    18721 ccatttccac cccatttata aggtttacct agatgttttt ttgcttctga tattactatt
    18781 ttagctttat cagatacatt accagaataa ccaccaataa taatttttcc ttttcttctt
    18841 ccaaatttac tagcttcttc aacatttcca aataaaatat ctatatggta tgttccattc
    18901 ttttctatag ttattgcagg tcctacgtca tttactttat agactccatc acgaccagaa
    18961 actcctgtaa ttacctgtat ttcatctcca tagtttaata aaggatgttt atttaaaaac
    19021 tcttttgtat aataagtttg ctcatattta ccaaccatag gagcagcgca agttttttta
    19081 gatgggtcaa gttttttctt tctacaatct gtatctccac cttcttcttt tctaggacag
    19141 taagctgtaa attctgctgt aaactctttt cctcctgcat aatcttctcc ccctaaatta
    19201 ctttcttcct taggttcatc ctgtcctgct gacttttcat ccataagatt ttgaaaatta
    19261 agttcaagct caatttgata ttctccattt tgccaagtat gtttgtctgt atctatataa
    19321 aatagtccta caagctttgt ataagaatct ttaactttta ctcctctacc agttatacaa
    19381 cttacatctc cataaccttt aagagaacag cttttttcta tcccattaaa ctcgctatca
    19441 atatctacat cttgattttc ttgttgctga attacttttt gcattattac atttacttcc
    19501 ttaaaaattt cattatctat tttttcgcta atcttgcttc catactggtc taccactatt
    19561 actttatttt ttacattttc catgctctcc gaaaaggtgg tatttataat attaaatccc
    19621 tcttcaaaca taacacttaa tgtaacagtt cctttttcaa taacattaaa cttatctaaa
    19681 ttggcctcta tcatatactt tttctttgtc tttttacttg cttctgtata tgcactcatt
    19741 attgtgtcat aaccatttac tcctataaac atctttgtat acttgacatt ggtctttgct
    19801 attattccaa ctgaaagcct attttcagca aatacttgct ttgctatgtc ttcaactaac
    19861 ttatctttaa aattgtatga cacttcactt tgtgtaagta aaaatcccat atctttagat
    19921 acaaaactaa tttcattact gcttgaatct ttagacctat taattatcat tcctctaaag
    19981 agttctttct catctacata gaaacagact gtactagcta ttggtatatc gatttgttgg
    20041 aaatttacat caaatgatga ttgtattatt gaaaactcta gtgtccttga tggagattta
    20101 taatcacctg accatgatac cttgtctact atgtcagtta tgtcatatat acttccgttt
    20161 tttatgtgta cccaaatctt aataataaaa aatcacctgc cttttcattt aattctatca
    20221 gcagttattt ataacttatt atctataata gttattgttt tggagttact tttggtatct
    20281 ttattctttt atactctttt aaatctaatg taaaatatac atctcgagtc ccatctcttt
    20341 cttcataact aaacccttca attatgactt ccatatttat atttgtttcc gtaattgtaa
    20401 atcttaatat aaaaccttcc ttcatccatt tttctatctt atttacacaa tcatatggtg
    20461 atggaaaacc tgtatagtca cagaaagtat agtctttatt tggaaaaaaa cttgatattg
    20521 aaatgctttt aagagctaca ccaccaaaag ttgctatttc acctattttg agtatactag
    20581 aagagtttac agcagctttt ccattaatac taaaggaaga tggaaatact ggaaatctaa
    20641 atctatcttc agcttgtcta agccacattt ccattataag acacccccta actgtgccaa
    20701 ttggatactt gcaactaatt ttgatgttat cttttctata tcagcttctt ctctaatcac
    20761 tattgtatca gctaacttag ctatgtttat atttccacca ttttttctgc ttctatattg
    20821 atttgcttct tgtttagtta aaaccatttc accttcatgg agtctggttt gatagtcatt
    20881 ataaggaaca tagttcaatc caaatgcatt attacctttc ttacctgaat aagctttacc
    20941 agaatcacca gcctttgttc cagcatatgg attatctttg taattttttt tactcttttc
    21001 cactagattt aaaactgttc ctgtgatgct tccatttttt atcttattaa tcatagtttg
    21061 ccaccattta catatatctt taactatttt acatacttta tctaatatgt taaaaaaagc
    21121 tcccaatatt ggctctataa ttttccatcc agtttccaac agaggtccta aggtctccca
    21181 tacagttttc caaataacac ccagtgtctc tataaaattg tttatttgag gtgcatattc
    21241 acctaaaaaa gtaaaaattc ttgtgataat aggactaaca gcttcaacta caggtccgat
    21301 tagttggaat gctgtagtta ctattgtacc aaatatactt attaatcctt gtgcaattgg
    21361 tgatttattg tttatactat ctagtaaatt atttactaag tttactactg gaagtaatga
    21421 atctattaat gctgtaaaat cgatattttg taatagtgta cttcctgcgt taacaattgc
    21481 ttttggtgca ttccctatca ttgttgctgc atgattttca aaacgttctt gcttattttc
    21541 aaccatcatt ttacgctttt cagcctcatt agaaattcga tattgagtag aatcagaaaa
    21601 ctcttttcca ttttttacac ttctagattg catctggtca tatttgttgc caataattat
    21661 cttaccattc attacattgc ctattttacc agccattttt tcagataagc cagctaaatt
    21721 gcctaatcca ctttctataa aagtttttat tttctttagt ccttgaccta aaatatcatc
    21781 aaaaggttta acaatactct tcaagccatt gttaatgctt gtttgagctc ctgatattat
    21841 acctggtatt gtattactca tttctttagc taaccccatc tgctttacac ttatgccttc
    21901 caatgcttca tcaaaagttt tataattttc tcctaccaga gtattaagag attccatgtt
    21961 tccttttgct gcacttaaga aagctgctga tacatcttgt tcactacttg tattcatatt
    22021 taaagctcta acatctacca tagcttgagt tatatctttt tcttttttgc caccagtaat
    22081 attactaatc attttagctt tagttcccat tgcaacaaca tcttcagggt caaaaggcgt
    22141 atcatttgcc attcttacta aactgccata ataatcatca acatctattt gattaacttt
    22201 tccagtattt cttttcatta cactttctag aacaactttc tgattttgtt cctgaccaag
    22261 taaatcaaca ccctttttca cagcgacacc tatagtagca ccatttgtca aatcagatat
    22321 cttccctttt atcgaaccaa gtattccact tgccatatcc tttacagcta ttgcaccact
    22381 ccatacctta cctgcaaata atctcaattt gggaaatact ttacttataa tgccacttgc
    22441 catatctttt atagctatta caccactcca aaaagtattt ttaaacaaca ttaacttagg
    22501 caaaattcta cccatagcac tactcaccat atctttaaga ttaatacttc cttgataagg
    22561 acgtttctct acttgtttta attttcccaa gatactactt atcactctct tagtcatatc
    22621 ttttgctttt attacgatat tccaaatctt gccaccaatc ttttttaaat ttccaagtaa
    22681 tttattttta gttttttcta cttcatcact tttagcttta aatattaata taatcataat
    22741 atttgaatta aactcatttt tcttttgaat caacttatta aaataatcaa taatcatagc
    22801 tatagttatt gctagtctca taatacttcc atttaaactt ccaataactc ctgaaacagc
    22861 atttatgcta gaacttaatt ttgtcatagt tgaacttaca ctactagcag tagtttgaac
    22921 ttttcttaac gaggaattta aactgtttac tctattactt gcacttccaa tagaattttt
    22981 tatgctacta gccattacat tagtggaaag tgaaacagcc acagttgctc tcctaaaatt
    23041 atcagttact gatataattt gattcatttt tggtgtgtaa ttatctgtaa ggtacatatt
    23101 tataccttct tctctcacat ttcccattac ataccacctc ctgtcaaagg acacactcca
    23161 tatccatgtt tcattttttc aacttcttcc tcaatttcta aggcgaaaaa agcttcaata
    23221 agctttaatt cgcctttatc cattgcataa aaaagggacg gtcttatacc ttttttcttc
    23281 caataatagt acattattgt agtcctggta tccgtcccta ttagtttttt acctcttcta
    23341 tcactgcatt tttaccataa cccataacat cttgaagtat tctatataag ttggtaattt
    23401 cacctggtaa taataatttt cttgctaatt cttttggtgt tggagcctta aatttatcca
    23461 ttagctcctt atttttaaat aaaagctttc catccaaatc acaaacacct tctataattg
    23521 taagcatctg catctccggt aagtctatat ccgttttcat attttcatca attttaagac
    23581 aagtattttg tatttcttca tctttactta ttgttaaagc tctacacatt actttaaatt
    23641 cctgcccaaa tatagttgat aatctcttaa gttcaactat agtacttggt ctttctaagt
    23701 tttctgtatc tgaatttaaa agtaaatcta ctatgtttat attttttgat aatccatttt
    23761 cactcattat tatttgctcc tttatcaatc aaattcaatc aactaacttt tttacttatt
    23821 aactatctga atttattaag tatatactta ctaaatatca gtataaaact aaatataaaa
    23881 ctaaattata tcaagaaaat catactcagt aaatgtgaaa ggagcttcta tctctccttt
    23941 tactccaacc tcccaatcag ctaaagttaa atcatcaaat gctatatttt ttactgctat
    24001 tctttctgct ccataagaat ctgggtcatt taattttgat atagcaacaa atctaggttc
    24061 agaacctctc tttatctttt ctccaattaa cttgtgcatt cttgaactaa cgtggtatag
    24121 agttattgag ccttttcctt tatatcccat atactttgta tcagtaccca tttgacctgc
    24181 tattataata tcttcttttg taaattccat ctttgcttga aatttcttta cttctgctac
    24241 tttgtttcca tcaagccaaa gttctcccca agtaccactc attacatttc tagcttccat
    24301 atttgccata ttaataatcc tcctatattt ctattgataa atctatatct tccatagcat
    24361 caagtacttt tatttttgct tttaaaaata ctttagaacc tgtgttagct tcttttattt
    24421 cttgtaatgt catataagat aaatctactc cttttgattt taaatacgat ttttgtgctt
    24481 caaaatctat ttcaacagta gaatcagatt ctataagtgc tgatttttct aattcttcta
    24541 aataactttt tatagctact atcaataaac atttgttgtc ataactgtta gtaacctttc
    24601 ctatatagtc atctattatc acctttctta tgtcactatg tataatatct aaagtgtcaa
    24661 ctatttttat tttctggaac atttctcctt tttctgctgt taattcagtt aaagaattta
    24721 ctcctctagc aattcttata gctcctgctt ctttaataag tataagctct cctttattaa
    24781 cccttgattc tgcatcaact ttcgtcatct taggtatatc gactacatca ctaagtttag
    24841 tataagttac tgattgactt aaaggtgtac ctgctataag tccagccacc ctgcttgtaa
    24901 actcatcaac actgtatttc ttttctccaa ctaacacatc ttctgtagta aaattaatta
    24961 tcccttcatg atttcctaca acttttccta atacagcttt aaccttaacc ttatctatat
    25021 ctctaagttt aattatccaa ttttttatag cagtcttatc agcttctact gcttttggca
    25081 tacatagata attaaattcc ttagtctcta aaaaatctaa tgcagtttga atatctgctt
    25141 ctccttctat tacataaact aataatttat ttggagtgtt aacatttccc atcaaagcta
    25201 aatttatata ttctttattt tcagcagata aatcaactgg tatatcctct ttttcatgta
    25261 tttcattaag acctagtgcc ttagcatctt taagtaccat tgcaattatt cctctagctg
    25321 aacgttcttt aacagttgta gctagctcct taaatgatat gttgatactt ggtaatccta
    25381 tagccatact atcactctcc taaatttaaa ttgatttctt tcatattttc atatactaca
    25441 tcttcttcat atacactatc cagatagttt agtgtaaatg taaagttcaa tacataaatt
    25501 gactcttctt ttttaatact ttgttctagc tttgttatat ttaattttct gtctgttacc
    25561 tttatatttc tgttaaatag cttatttaat tcatctgaaa tttcatacat tctaatactc
    25621 tttgactgtg gttttggtaa gtattttata gagataacaa tatctttgtt attcgtattt
    25681 aacattacag gtgtacaaat aactgatagt atacttacaa aaaaacaaga cccttcagag
    25741 ccttgcacac tttcatcatc aataaaaata ctttcagtaa agtttttttt gagaatctca
    25801 ataattgttt ctaatatttc cttgtattta agcaaataat caccacacta tcactaaata
    25861 ttcttgctta atctgataat cttactataa atgatataag tcatttttaa atatatatct
    25921 catttattcc tgtcaagtat cttctatatt tttaggtaat attattcttt tcaatctatc
    25981 acctccttca atatggattt ctttcacaat aacattatct cacatatttt attgcctatt
    26041 gttgcaatgg tgtatataaa gtgttgtaaa attgtcctat tttttataat atctcactca
    26101 atttaaatat catctttttc tttaaattgt agcaagatgt tctatctaag tgtagtttca
    26161 tagaaatata tgtcatattg ttttttgtct tactattata aaaaagatta aaaaattcca
    26221 tttctatatc atttagacac gttagagcat tttctatctt ctctttttct atttctatat
    26281 cttctttttc catttttaat tttcttaatc tttcctctct ttttaacacc tcattttcca
    26341 cagaagagct tatgttatat gtgttactag tcttttctgt atataccatt gctccacatc
    26401 cagagtattc attctcacat ctttttatgt ccaagttaat attctttatc tttatatcca
    26461 aatacttata attatgtaat cttttatctg tttctttaaa taatatatct tttttgtttt
    26521 ccatagtact tccccttttt tatatactta ttattagctt caaatttaca ataatctctt
    26581 tagtgcacag ttttttacta atctcacaac tttcaagaac atattgttct ataactgtat
    26641 aaaaaaaact tcttctatat tttgttctaa aatactagaa atcttcttag ccataagaag
    26701 tccaggattt tttatacctc tttctatttg agatatataa tcatttgtaa ttccagcctt
    26761 tctggcaagt tgaatttgtg tcataccttt gcattttcta taatatttca agttattttt
    26821 aaacattgtt ttatacctcc attactatta agaatatttt gttcttcctc acttagatta
    26881 tatagaatta tttgttcttt atcaatagat tttagaaaaa aatattcttt tatattttat
    26941 tagttttatt ttttagaaca atttgttata atctatacat aaggtggtgt tataatgttt
    27001 gccaaaagat taagagaact tagaaaggaa tttggattga ctcaaagaga acttggagaa
    27061 aaagtaggcg tttcccaaag agtcttaggg tactatgaaa cagaaaacag atttcctgat
    27121 gaacacattt taaataaatt ggctgatgta tttaatgtat cagttgatta ccttcttgga
    27181 agaaccttag ttaaggaaaa tattgataca gtagctgcac atagaaaaaa tccacatgaa
    27241 gagttacctg aagaagcaca agaacaactt aatgattata tagaattttt actaaataaa
    27301 tataaaaaaa aataaaccat atgatgagca gtctattttt ctgctctttt aatataattt
    27361 aaaggcaaac atacattcta ttttaggggg atttttatga ataatttgga taagcttttt
    27421 gaattagctt ctcaagaaga gataataatt cattatacca cttatattgc aggtgattta
    27481 gaaggactat atataaacaa acatggtatt aaaattatat cattactcag taatttaaaa
    27541 caaaactcaa aaaaattgac atccatctta gctgaagaac ttggacatca tttcactagc
    27601 cttggatact atgtatcctc ttataacgat tattatacaa aaattattat agataagtgc
    27661 gaaaataaag ccttaaaatg ggcttgtgag ttcttaatta ctgaagaaga tataataaac
    27721 ataattaact ctggcatcac ttgcgtttat gaaatggcag acatacttaa tgtggatatt
    27781 actttctttc aaaaaagatt agaatttcta tcattaaaaa aacagtcttt acaacttgga
    27841 aataataaat atttaatatt aactaatttg ccgtatttct acatatttga tccaatttca
    27901 taataaaaat agtagtaatt ttaactggct taacagtaaa atctatatat ttattttaac
    27961 aaattttggt ttctataaat tcaatattaa tatataataa tgatatcata ttatttaaaa
    28021 ttctaaaaac aaatatatgc ttttgttaag aaaggttgtt tgatgaatgt ataaagaaaa
    28081 gttgtttgat aattatttta agttcttagc actattattt tggccaatta tgtggtataa
    28141 gtggatagta atttcaaatg gtactcttga aaatatgtta tttaccatat atgcaatcgt
    28201 cgcaattgtt ttcatcattt tatattcggt gtttatgata aaatataaag acattacaca
    28261 aatagatttc ttttacagga taagtactct acttgctttt atatttacac tttttagctt
    28321 tttaatatac ccaaagagtt tatttttctt gtatttaaag attattttta ctggaatata
    28381 cctttattat tcaatagtaa aaactcttaa atttaaagat gacgaaggtg tagtgggtat
    28441 tatgagctca cttttattaa ttgtaataac tttgttctac taaatttaat tctatttgac
    28501 ttaatatggc tttaaatatt agacatctac attattttct gatattattt aagatggtag
    28561 tcttatatta ataatgaaat acactatgaa ggagttaact attatggaaa aaaaagtttt
    28621 agcaacagtt ggagaaaaag agataactaa tattgatatt gaaaatgcat taaaatcatt
    28681 agacccttat caagctatgc aatttaaaac tgaagaagga aaaaaacact tattaaatga
    28741 tttagtgaat caagaattat tcttcttaga tgctaaagaa gaaaaattag atgaagaaga
    28801 aatatttaaa ttagaaatga aaaagataga agaaaatgta ttaaagcaat ttgctataaa
    28861 taaagtttta tctagtgtta atgtaacaga agatgaaaaa gtaaaattct ttgaagctaa
    28921 taagagctct ttctctaaac cagaaagtgc aactgctaaa catatattag tagatagtga
    28981 tgaaaaagct aaagaaatac ttgctcaaat aaaatcagaa gaaatatctt ttgaagatgc
    29041 agctcttaaa cattcttctt gcccatctaa agatatgggt ggtgatttag gaacattcgg
    29101 tagaggacaa atggttcctg aatttgaaga agctgtgttc tctatggcta aaggtgaagt
    29161 aagtgaacca gttaaaactc aatttggata ccatataata aaattagaag accttcaaga
    29221 aagtacagag tctacatttg atgaagttaa ggctgaagtt gaaaaaagtt tattatatca
    29281 aaaacaaaat gaagtttatg gaaataagat aaatgctctt aatgctaagt acggaaacct
    29341 tgtaaaatac aacgattaat cataaggtca tatataaaaa agctgtcttt tcacaatatt
    29401 tattagaaaa gacagctttt tatattaaag tttaaatatt cagtttcata ttatcattct
    29461 tttattgtaa agaactcagc ttaaaagttt aaataagatt tttaattatc attagctaaa
    29521 tttttatatc cacgtattat ctcataacag taacctatta catctcttct ttttattatc
    29581 ccaataaatg tgtcttggtc atctataaca ggaatgaaat tttgatttaa agattttata
    29641 actaaatctt caatatctgc gttgatggaa acaggagaat tatccatttt tctatttata
    29701 tctgtcactg gaacatcttc caatcccttt aagtccagat ttaaatcatt ttttaatgtc
    29761 cataaaaaat caccttctgt tatagttcca acatatttac catccttact tattattgga
    29821 attgcagagt atttgtgata ttccattttt tctagggctt gccttatagt gtagtcttca
    29881 tatatatatg ctacttcact ttttggtgtt agaaagaata atatgttcat tacactaccc
    29941 accgttactg aaaacttata aatattactc aatatttata atgttacttt tttgcagtaa
    30001 ctttctcctt tcttattttc taagtattct taatactata cgctgtaaca agcgtttttc
    30061 ctttgctcat tttaatctta ttatgcttta ttaatatttt tatatggtct atcagtatca
    30121 cttattctct ttttagaaaa tttactaaaa attaattgac ttaaacttag ccataataat
    30181 tttactctac actattaatt ttaacatatt tatctcatta ttaatgattt tattttaata
    30241 attttaattt attttgctat taattttatt tacaaaaact gaaaaaaccg ctaaatgatt
    30301 agcggcttat ctctcatatt actagaacac taatatatat gaatttttaa ttataatcta
    30361 actacgttag cagcttgagg acctttagct ccatctacta cttcgaactc aacgctttgt
    30421 ccttcttcta atgttttgta tccatctcca gttatagctg agaaatgtac gaaaacatct
    30481 ccttcacctt ctacagatat gaatccaaat cctttttcgt tgttaaacca tttaactaca
    30541 ccagttttca ttaataaatc ctcctaaaaa aacacaaata tttagctact cttttctgta
    30601 accttattac tactctatca tatatagcta tgtttgtcaa ttattattat ttttatacat
    30661 taataatcaa tttttttact ttataatttt aaatgattat tatcttttac actttatttt
    30721 tctttataat tttttaataa tttattttta ttatatggaa tcttatggac ttatcactcc
    30781 aacctaaatc ccatggcacc aacattctat cagtattatg acaagttacc aaaggatacc
    30841 ctcttgagtc taatcctgtc actgtcgaaa catgtgttat tctgcctttc ttctcataac
    30901 caacaaaatc ccctggacgt aaattataag cttctttgta tacttcttca taggaacctt
    30961 tagcaattaa tgaacctctt ccactataca gtgcataatt tttaaaagct tgtgcattta
    31021 cccatgcttt tgtacctgct ctctcgctat aattccaaat tgagttcttt ttgaaccttc
    31081 cactttcaaa cattatctgt gatgcaaagt ttgcacaatc tccaccttct ggattataat
    31141 ccctatagtt tggattaaat ttcataccat gttcttcatc agctgcggct ccacagtatt
    31201 tatgtgcata atctatagcc tttttttgtt catctgtaag ctgtaactct acaccatctt
    31261 gttgaccaat ataatttctt atatcatcag attttatatt ttctaaattt aaagagtctg
    31321 caaaagggtc tgtataccac tctttagtta tgacatattg attatctata atttttacat
    31381 ttagataatg atatgtacct attctaaagg cattttcttt gtccttttga ttttcatatg
    31441 aatacttata ttcagtcgat actgaacaga ttataccata gaggtctttt tctttttctt
    31501 ttattttttt aatcttaacc cttgtcttta tgtctttaaa ttttacacct tgcttactag
    31561 accaattttc taaatacttc atctttttta cttcattttc ataagcccac agaccaaatt
    31621 ttttatccgt ctcataaaga ccttttaaaa tgttttcatt tttctctaat attgccttat
    31681 ttctgtaatc aaataagttt tcaagcaata gtttatattg gtcttttaca tcatcacctt
    31741 caacatttgc agattccatg actttttctt ccatattttt catacttaaa tcattgatta
    31801 aaaatacacc tgctgtagta gctaaagaca aaaatacaaa tgccattata cctttgatgt
    31861 acttattctt atttttcagt attttcaaag ctacacctcc caaatagtac accttacaaa
    31921 tcaaattaca aagaatattc tgtcacatat agtaattttc atatttaata attctctatt
    31981 ttattatgtc caatattaaa aattaattgt atcaatacac tgtttttttt acaatttttt
    32041 attttataat ctactttttt ctttttttta tagtaatata tatgtatata aatattttgg
    32101 tatcattata attaatacat actaatggag ggttttttat gcaaaaaaaa gtcgcagcaa
    32161 ttaatgactt atctggaata ggaaaatgct cattatcggt tgctatacct atactttcag
    32221 cattaaaggt tcaatgttgt ccatttccaa cggccatact ttcaagtcaa actggttatc
    32281 ctgaatttac ttttttagac tttaccgatg aaatggtaaa atattcaaat gtttggaaaa
    32341 atttaaaagt caattttgat agtatatata gtggtttttt gggttcaaag catcaaattg
    32401 agatagtggc taattttata aacgattatc ccaatgcttt tatcgtagtt gaccctgtga
    32461 tgggtgataa tggagttatg tatccaatat tcacagaaga aatgagacat gaaattaaag
    32521 aattagttaa acattcagat ttaactactc ctaatttaac agaagcttgt ttcttaactg
    32581 gaaatgatta taccaaaagc gattataata gagatgagct tatatatatt gcaaaatctg
    32641 tatctgattt gggacctagc aaagtcgtta ttactggtat attagaagat gataatatat
    32701 taaatttggc ttacgataga gataatgacc atgtattctt tacatcagtc aaatataata
    32761 attgttctta tagtggaact ggagatatat tcacatctat actttgtggt atgttagtaa
    32821 ataaacatga tttgggagta gcggttaata ctgcaacaga ttttatatat aaaactataa
    32881 attacacatc tcaatttgat actgatagaa atgatggagt tatgtttgaa aactttttat
    32941 cagatttaac taatatataa agtcaaaaaa actatccatt ttataaatac ctataaatca
    33001 taaaatttag gtatttacaa aatggatatt atttactaaa ctattggtat ttcaattgtt
    33061 accatagcac caccagtttt cacagaattt tcaatatata aaatcccatt atgtttttgt
    33121 acaatattat ttacaatata gagtcccatt ccataatgag atttagaatt tctactcaaa
    33181 tctcctgtat aaaactgttc tgtagctttt gacaaatctg ctttagaaaa accacttcca
    33241 caatctgtaa ctacaaattt taagtattta ttacgtactg atacacttat gtatagttta
    33301 ctctggcttg gtgaataatc tatagcattt gaaattatat tcataatagc tctgaataac
    33361 agttctctat ctatagttat atttttaggc aaattatctt gtttataaaa ctccacatct
    33421 aagtttttag tactagctaa tgcttctatt tgcactaaga catcttttat aaactcactc
    33481 atactgattg cttctttatt taatatatag ccattctctg tttttgatat atctattaaa
    33541 gtattaatat atttttctat ttgagtgaca ccctttgaga tatattccat gtattctaat
    33601 aactcaggat tttcatttat ttcaattaat aagtcagtat ttccatgtat aattgttagt
    33661 ggtgttttta tatcatgtgc cagtgcagat atctgttcct ttctattttc ttctagcatc
    33721 cattgttttt ctaaagaatc ttttaacgcc aacttcattt tatccataga aaaaaggaca
    33781 ttattaattt cataaatttt actagattct acaacaaaat ctaaatcctg ttgttctatt
    33841 ttttctgtag tattttttaa aagctccatt tcaatattaa atttcttgcc aaatactttt
    33901 gataatataa caatttcaat aaaaaatatt atactaaaaa taatcatacc caatgtctct
    33961 ggttttggta aatatgctct taaaattgga gaactatact ctgatactat tttgtatttt
    34021 attataaaaa cttcatcttt cttaaaaaac tttatataac tttctgaata tgcatgcctt
    34081 tcttcaatat ctctcattaa attccatact tcttttgatt cttttttatt aaaggtccct
    34141 gaaattaaac tcccatccaa agtataaacc ccatactcat atatacttgg aattatatct
    34201 tcagttactt tttcacttga aataatctgt tctttatatt tattaaattg tttttctgca
    34261 taattagctg gtaaaattac acctgatgat aataatacag aaaatgataa cattaaaagg
    34321 agtacaattg aaattgtcat aataaagaat aaagataaat attttataaa aatagttctt
    34381 aacttttcaa cttttttgtt gttagatttt ttacctccaa gcatttgtac cctgtcctcc
    34441 aatagtttaa ttaattcata attaatatct aaccagtttt ttatttgatt attttatatc
    34501 tttttattgc taagttatat tttaacataa taatataaat aaacagtcaa tttttactct
    34561 attcttctga ttttttacct tcccatttag aaaaccaaat acataaaatt acaaatgcta
    34621 aaattgttac aaaaccacac attaaacctg ttgattgata tgcttttatt gattcaacct
    34681 ttatatattc aaccgtttta gaactcgaaa aagacgacca aatagttata aatctaacac
    34741 cccatgcaca aggaaaatac tgccaaatac cagaacccaa tccagttaat aataatgctg
    34801 aaagtaacat ttcaactata cctactccaa ttgatgttcc ttttccgaac cttaaactta
    34861 agaataaatg taatatgtat tcaaataaat tacacccaat taaaattaga attgaaatca
    34921 tataaaagct tgatgcaaag taagccttag atataccaaa tttaaaacct aatacagaca
    34981 ctaatgttgc tacaaaacca agacatacta agattatata tttacttata agagtaagat
    35041 attttggatt agatgttgtt aacatatgtt tataattccc tgcataatat tcttgttcaa
    35101 caaacattga acacacaaca cctgacaata aaggaaaagc tattgctata acttgtaaat
    35161 atgctactat ctttagtata ttatcagctc ctgaaacctt ataataccat aaaaacaatc
    35221 ccatccccat taaagcaatg caaatgtgca ttagtattat aaaatttctc tttaatttta
    35281 ttaaatcaga acaaaatatt cttattaact gtctcatatt acttcacctc tctattattg
    35341 aaccaaaccc ttgtagctaa agcaaataat acaaataaaa caatagatat taatactcct
    35401 ggcaaaataa cagcattatt taaaaattca cttccctctg gtacaggaag accatttggc
    35461 agaatatgca atacaacagc cattaatctt gaaggaatcg aatatggaaa catataccac
    35521 atttctgtag ttgagaaaac agcaactcca acaatagtaa agaacatatt tattagaact
    35581 gtaacaaata taccaaattt tgaagctaaa aacatacaaa gaggaatctg ccacataaat
    35641 agtaaaataa ttaatatagt tgcaatgata ttttgaccta ttgaaatagt ttctttatag
    35701 attatcccaa caaaaactac acctattgca gaaataattg atgctataaa acaacaaata
    35761 atacagtaaa tcatttttcc agtccaaacg tctttcattt caattggcaa tggtaaaata
    35821 ccacgatatt tacttttttc atcttttaat actattaaag aagatattaa tgttaatgct
    35881 cctggaagaa atgtcgtata ccaccagttg aaagcagcat tttgccctcc acctagtcca
    35941 cataaaagta tacaaaacac tggaactatc catatcaact tcattccaaa agttcctttc
    36001 atcttttgaa attccactct aataaatctt tccatattac tgccctctct ttctattttc
    36061 agctacaact ttcataaaaa gtttttctaa gttttgttct tttgatactt ctccttgata
    36121 acccattact ccattagaaa ttattcctat ttcatctaca acttgttcaa cttcagacaa
    36181 gatatggctt gataaaataa ctgtaattcc ttgttttgga aaagaacgaa tcaattctct
    36241 aagttcttca ataccaatag ggtcaagacc atttgtaggc tcatctaaga ttaaaagttt
    36301 tgggtgattt aataatgcaa tagcaatacc aaggcgttgc ttcatgccta gagagaattg
    36361 acctgaacgc tttttaaagg tatctcttaa atctacagtc tctaaaactt cttcaatgcg
    36421 agagtcagga agaccaagta aagtagtatg gacttttaaa ttttctcgtg ctgttaaatt
    36481 ctcatatata gcaggagact caataagaga accaatattt gataaatcct ttctagtcca
    36541 tctatgtccc tcaaacatta tttttcctga agttggattt aaaatcccag ttatcatttt
    36601 taataaagta gatttcccag ctccatttgg tcctaataat ccatatatag aattagattt
    36661 aattgctagt gaaatgttat caactgccat ctgctcctta aattttttag aaactccttt
    36721 tacctgcaaa atataattac tcataatttt catcctttct tttgtcaaac ttatttacaa
    36781 gaatcattct aaatcataaa tataaggatt ttataagaat taaaaaacaa aaaaagatgt
    36841 gttaaaaaca catctttttt tatcttaatt ctattaattt tccattgtag tatatctcta
    36901 cttcttcaat ccaaatgcta ttatcttctt cttggatgac atttttaata atattattta
    36961 tagtatatat tatattttgt tcattcaaat aatcatcttt ccaagaaact ctactttttt
    37021 tatctggttc tatcaagttt attttaacaa gattatcttt ttccttaact tctacttcca
    37081 tgtctaaacc attaaaatac tcatttgata atgtatctaa gattaatcgc agtttatcct
    37141 taagtgaact ttcttcatca atctgtaagt actttactat tttctcatcc tttgaaatta
    37201 tctctatagg tattttagta gtttttttag aattaatcat tttactagtt gtttcttctt
    37261 ttgcatcttc atccttcttt acaactgctg attctttaga atcaactagc tcaactgcac
    37321 tgatgctata atccatacat gccgtactca ataaagtagt agcaattatt aaaaaactta
    37381 ctgctatttt attcataagt atccctctcc taattctctt tcgatactta ttataccata
    37441 gtatagatta tatttttata cttttaaatc aatttataaa ttattatagt aaattattta
    37501 caattctaag ctatttgaat ttatttttat agtatattct ttctctaaat tcgacagatt
    37561 tttattcaaa agccctttta tttctctcgt atttctatac ttgttgctat gttcttcact
    37621 atgttcataa tcatatgaat cattgtcata ttcttcatca ttattaaatt cttcaattgc
    37681 ttcttttgac tttatatcac aattaaattt atacttattt actggtatat caagtcttac
    37741 cttagaattt ggaacttcta tgtccaggaa cttagcaatt ggcatatcag agtacaagta
    37801 aaattcactt tcataatttg aatttctacc tattttctct tttatattta cattttcagg
    37861 tatatactct tctatattat tgataaatac atcttcattc tctgaagata tatacaatga
    37921 ctctgattct atgtttacat ttttaacgcc taaaattgat tttgttgaca agtgaagatt
    37981 gttattagat attaagttta ggttttctaa tctatttact ttgttctctg ttatagctct
    38041 actaaaacct ccataagaag ttttatatgt tatagtattt gaagctacat tatcgtatac
    38101 aaataagtgt gagcttatac ttgatgcatt tatgtctaca ttatttggta catatattat
    38161 aatttctcta taatcatgtg aaaacatact atttatagat gtatttaata attggtcgaa
    38221 atttttaatc tttttaattt tattttcata ttttctttca ccttttacaa ctaaagtctt
    38281 atcttttaaa gttacattgt atttgtaaaa ctcattattt cctcttgttg ttacacgcac
    38341 atcttcactt gaacttttct ttattgtaac gtttatattg tcaacagcta tatctaactt
    38401 gtcaatattt actttttctt gataaagttt attttctttt ttaaattcag cttccttttc
    38461 taaaccaaag tttattaggc ttggcataac taaaatccct gaccatgttg tccctattat
    38521 acctattacc aataaaaccg cagcaaatat agcaagtttt ttattcatgt ttatcttctc
    38581 ctttcttatt ttctatatct tcaaggtcct tatatatcgc ctcaaaattt atactatcat
    38641 cattatattc atcagtttct tttatgcttt cttcttctct attgtagtaa tcattattat
    38701 atgaatcttc attaatgtct ttattcaaat cttcatcatt taaagtagct ttttttcttt
    38761 tttccatctt ttcatttgct tttatataaa tattttttct ctttatccaa tgtatataat
    38821 tatttagtaa ttgtttacct agttttatgg caaataataa aatttgccat gcaagtatct
    38881 caagccctac aaatgctatg gttgcgaaaa tacaaaacat cataacatct ggaaccattg
    38941 ctataaattt aatcacaaat ggaattaata taacaaaagc aattaatgag catactaatg
    39001 caatagtagc agatacaata gcacatacaa acgcaaatgc tggaacaatt agaaataaag
    39061 ataacactac taataacccc tttataactt gtctggataa tcctcctaca ccttcttcta
    39121 tacttggtgt aagtttatca tttatatttt cttttgattt attgtacttt tccttagctt
    39181 catctttcaa gatagaataa tcctttttta catttgattt aaatctacta aatttaccat
    39241 ttaatatgtc tgtattcatt atttttccat ctatattttc ttttgattta ctatactttt
    39301 cttttgcttc atcttttaag atagagtaac cttttttcat atctgattta aatttactaa
    39361 acttatcatt caaaacatct gtatttatta ttttttcctc ttcaatattt ttttctttag
    39421 tttgtaagat aagttctttt gctatagatt ttggtgagcc aagtgatgct attatctcca
    39481 tatcactctt tccctctata agaccatcta caaaatactc ttcataatct ctcaatatat
    39541 cattaacttc gctctcagaa aaatcttttt ttagataatc ctttaaaatt tcaagaaact
    39601 cagttttatt caagttcatt acctccctca tcttcactta ttaaaatctc aacagccttt
    39661 acaaattctc tccactcttc catcagattg gataaattct cttttcctag tgaagttatt
    39721 ctatagtatt ttctagcagg accttcatta gattctagta tatatgtttc gaaatacaaa
    39781 tcttttgtaa gtcttcttag aataggatat attgttcctt catttacatc tattacttta
    39841 gagatgttat gtactatctc atatccatac atatctttct tagatatgag agcaagtaca
    39901 caaatttcta aaacaccctt tctaaattgt gtgttcatat actccatcct ttctttaaat
    39961 acacagtatt gtgttttaca aggtactatg ttaaaacttc ttatatcctc ataataacac
    40021 agtactatgt tctgcacaat agttattttt aaatattctt gttgattaaa tccttctata
    40081 aatgtataat actctaatca gatatatttt atgaaagtgg tgataaattg gctaaaatga
    40141 aagatgataa aacaatatta atcctaactg ctcaatttgg tgctggacat ataagtgctg
    40201 caaaagcagt caaagaatgt attattgaaa aatatagtaa ctataatgtt gttatacaaa
    40261 actttataaa tgcaagtatt cccatgatga ataagcctat ggtaaaactt tatgaaaaca
    40321 atacaaaata tactcctgga ttatataatt actattatta ctttaagaaa tcgtttgatt
    40381 caagacatga tttatctcat aaattatata cacctaaact ttctgagtat attgctgata
    40441 taaatccaga tttgattatc tctacatttc cactagctgc tgcttgtgta aataacttta
    40501 agataaaaaa tcctgatata aatataccta cacttacagt tataacagat gttgtagata
    40561 gtatggaatg ggtttttgaa aatacagatt tgtattttgt tccttctcct gaaataaaaa
    40621 atagattttt tcagaaagga ataaatcctg actccataaa ggttactgga gttcctgttg
    40681 ataagagatt tcaaattgaa agtaaagaac tatgttgtga taaatataga ctattacttc
    40741 taggtggagg aagaggtcta tttgatatag atgaggattt tatgcattgg atagatgaat
    40801 ttattgaaga acatagtaat tctatagaaa ttacaatagt tactggtaaa aacaaaaaat
    40861 tatatgacaa tctaactcat aaaaaacctc taaaaaatat taaagtactt ggatttgtaa
    40921 acgacatgta taatctaata agagaatgtg acctaatgct aactaagcct ggaggagcaa
    40981 ctatatttga agctattcaa tcacaaactc cagtactcgt aaaaatgcca aaagttggac
    41041 aagaaattga aaacgctaaa tttataatag ataagggtct tggcatgatt tatagtgatg
    41101 acctagacct taaaaacata ttttataaat tagtatcaaa tgaatttgat tctataataa
    41161 actttatgaa aaaaaattta aaagaattta aaacagtcat tcatccagag aaaattgctg
    41221 attatatatc agaattaata gataatcatt acagttaaat tgtactatta aaaaatttca
    41281 aacataaaat taaaggcaat caagtatgat tttacacttg agaatttact catagtgttt
    41341 aataatactt gattgccttt atcatatcaa aatcaaattg ttatattagt atatattttt
    41401 cttgtactgc atatacagtt gttgtatttc ttccatactt ctctttaatt ctatttgtaa
    41461 atctgaagca gtctggtaat ccttattttc tatagcttct ctcactctag tgaatagtga
    41521 ttctctagtt aaagtatcct tcataatatt gaactttaaa tccgatattg agttccaaac
    41581 tacctcatca agcgcatcca tattttcttt tgtgtgtatc ttgacaatac ttcttatttt
    41641 ttgaatccca gcctctatta ttctagtttt taatttcttt tgccacttat ctatagctcc
    41701 aatcttaaat gattctatta tactatcagt aaatacatca ccttgtttta aagattttaa
    41761 ttttgaagca tatatttcta agtttttcat attttcataa actgtagctg gtggtattcc
    41821 aaatcttgca tttctttctt ccaaactata atgttcaaat acatcatttt catctctata
    41881 tgctctatct ttttctaaat agaaactttc ttcacctaca tttttagata actctttttc
    41941 aagttctttt gtactaagac cactcttagc tgctgctttt ataccatcca acattgtttg
    42001 ataacatcct gctattacta aatacgtgtt agatagaggg cttggtgacc ttaattcaaa
    42061 tcttacagtc tttggatttt tcatatctct tattaatcca acaagcactg acctatttct
    42121 tgatggaatc tcataactat gccctaaaga tgtaactata catacaggag cttcaaatcc
    42181 aggaactaat ctattaaatc catcattaga agctgttaca attgggttta atacttcata
    42241 attgtaaaga agtcccataa gagccccata tcctagttca cttagataat cttcttttaa
    42301 atcttttgga gcaaatagat tttttatact tccatccttt aacttcgcac ttactcctac
    42361 atgtgtatgt ccaccacttc ctgctactcc atgaattggt tttgctttaa atgtaacttc
    42421 tagtccatgt gatgtaaata catcctctat aacatctctt actaataatt cattgtctgc
    42481 tgcttgtaat ggtgtagaaa acttccaaga aacctctaat tgctccatag catggtttgt
    42541 tcttccatct atactaatag aactttgtat tcctccgacc tctttgtgag ccatttcagg
    42601 gtttacacct agcttttgaa gtataattaa acttttctct aaacaagttc ttataatacc
    42661 atgagttctt ttccagtatt gctcttttag gctttgtgat acatataatt tctctaagtc
    42721 agctttatcc tcaggagtat taacccagaa ctcaagttct gtagctgctg ttaacattat
    42781 ttcctctata tcatctactg aatctattcc tatgttattt attacatgtg gatattctct
    42841 aaatatttca tacattgact ttttaaagta cttatctgct ttttgaagta ctcctctaga
    42901 acatactttt ttattatcat gtattaaaaa tgcaggtatc tttaatgttc caacaggtag
    42961 acctacttct tcgtctatat attccatatt gtaatctaca taccaatgac aacttttatc
    43021 tggcattaaa tctactttcg cattatttaa tgtagctata ttataaagct caacacttga
    43081 accatcagtc tgtatagctg attccaaaaa tttatctata tcctccaaaa ataattctac
    43141 aggaatcttt tcatctgttg catttcctcc taaatcaacc cccattaagg aaacaaatct
    43201 tatattttta ttgcttttta ggatttctct taattcttca ttagtgtgtt tatctttctc
    43261 tattacgtat aatagactat ccatttttcc actctcccat aatttttatg tatattttat
    43321 tttgttaaat acaagataaa tttcattttc cttacattta tgaatttttc ttgcaaatta
    43381 ttcgtgtttt attcgtgttt ttatttatat tattatatat caagtataaa aagttcgcaa
    43441 tattttttat tggaaaaaat attatacata caatagttta a
//
LOCUS       DAEMGG010000018        42900 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEMGG010000018 DAEMGG010000000
VERSION     DAEMGG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42900
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.018"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            37..498
                     /locus_tag="KPK24_000990"
     CDS             37..498
                     /locus_tag="KPK24_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298697.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            complement(591..761)
                     /locus_tag="KPK24_000991"
     CDS             complement(591..761)
                     /locus_tag="KPK24_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298698.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(950..1171)
                     /locus_tag="KPK24_000992"
     CDS             complement(950..1171)
                     /locus_tag="KPK24_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG2298699.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(1255..2481)
                     /locus_tag="KPK24_000993"
     CDS             complement(1255..2481)
                     /locus_tag="KPK24_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2298700.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(2543..3475)
                     /locus_tag="KPK24_000994"
     CDS             complement(2543..3475)
                     /locus_tag="KPK24_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG2298701.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(3564..4091)
                     /gene="hpt"
                     /locus_tag="KPK24_000995"
     CDS             complement(3564..4091)
                     /gene="hpt"
                     /locus_tag="KPK24_000995"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG2298702.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            4320..5396
                     /locus_tag="KPK24_000996"
     CDS             4320..5396
                     /locus_tag="KPK24_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2298703.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(5482..6690)
                     /locus_tag="KPK24_000997"
     CDS             complement(5482..6690)
                     /locus_tag="KPK24_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG2298704.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(6851..7240)
                     /locus_tag="KPK24_000998"
     CDS             complement(6851..7240)
                     /locus_tag="KPK24_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG2298705.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            complement(7344..8345)
                     /locus_tag="KPK24_000999"
     CDS             complement(7344..8345)
                     /locus_tag="KPK24_000999"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG2298706.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     misc_binding    complement(8404..8659)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8793..9983)
                     /locus_tag="KPK24_001000"
     CDS             complement(8793..9983)
                     /locus_tag="KPK24_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2298707.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     gene            complement(10264..11385)
                     /locus_tag="KPK24_001001"
     CDS             complement(10264..11385)
                     /locus_tag="KPK24_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG2298708.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            complement(11446..11931)
                     /locus_tag="KPK24_001002"
     CDS             complement(11446..11931)
                     /locus_tag="KPK24_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG2298709.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            complement(11933..12601)
                     /locus_tag="KPK24_001003"
     CDS             complement(11933..12601)
                     /locus_tag="KPK24_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298710.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            12991..15015
                     /locus_tag="KPK24_001004"
     CDS             12991..15015
                     /locus_tag="KPK24_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2298711.1"
                     /translation="MDKKKLILITHDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            complement(15245..16432)
                     /locus_tag="KPK24_001005"
     CDS             complement(15245..16432)
                     /locus_tag="KPK24_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG2298712.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(16634..17905)
                     /gene="brnQ"
                     /locus_tag="KPK24_001006"
     CDS             complement(16634..17905)
                     /gene="brnQ"
                     /locus_tag="KPK24_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG2298713.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILSYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            complement(18046..18699)
                     /locus_tag="KPK24_001007"
     CDS             complement(18046..18699)
                     /locus_tag="KPK24_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG2298714.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     regulatory      19028..19129
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            19202..20545
                     /locus_tag="KPK24_001008"
     CDS             19202..20545
                     /locus_tag="KPK24_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG2298715.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            20724..22088
                     /locus_tag="KPK24_001009"
     CDS             20724..22088
                     /locus_tag="KPK24_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2298716.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(22266..24131)
                     /locus_tag="KPK24_001010"
     CDS             complement(22266..24131)
                     /locus_tag="KPK24_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG2298717.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(24367..25584)
                     /locus_tag="KPK24_001011"
     CDS             complement(24367..25584)
                     /locus_tag="KPK24_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2298718.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(25784..26647)
                     /gene="aroE"
                     /locus_tag="KPK24_001012"
     CDS             complement(25784..26647)
                     /gene="aroE"
                     /locus_tag="KPK24_001012"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG2298719.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(26729..28651)
                     /locus_tag="KPK24_001013"
     CDS             complement(26729..28651)
                     /locus_tag="KPK24_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2298720.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(28715..29563)
                     /locus_tag="KPK24_001014"
     CDS             complement(28715..29563)
                     /locus_tag="KPK24_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG2298721.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            29976..30857
                     /locus_tag="KPK24_001015"
     CDS             29976..30857
                     /locus_tag="KPK24_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2298722.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(31065..31928)
                     /locus_tag="KPK24_001016"
     CDS             complement(31065..31928)
                     /locus_tag="KPK24_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG2298723.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(32083..34017)
                     /locus_tag="KPK24_001017"
     CDS             complement(32083..34017)
                     /locus_tag="KPK24_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG2298724.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(34129..35142)
                     /gene="galE"
                     /locus_tag="KPK24_001018"
     CDS             complement(34129..35142)
                     /gene="galE"
                     /locus_tag="KPK24_001018"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG2298725.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(35166..36035)
                     /gene="galU"
                     /locus_tag="KPK24_001019"
     CDS             complement(35166..36035)
                     /gene="galU"
                     /locus_tag="KPK24_001019"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG2298726.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(36233..36553)
                     /locus_tag="KPK24_001020"
     CDS             complement(36233..36553)
                     /locus_tag="KPK24_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298727.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(36543..38534)
                     /locus_tag="KPK24_001021"
     CDS             complement(36543..38534)
                     /locus_tag="KPK24_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298728.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(38657..41200)
                     /locus_tag="KPK24_001022"
     CDS             complement(38657..41200)
                     /locus_tag="KPK24_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG2298729.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(41478..41812)
                     /gene="rnpB"
                     /locus_tag="KPK24_001023"
     ncRNA           complement(41478..41812)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KPK24_001023"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            41944..42621
                     /gene="srtB"
                     /locus_tag="KPK24_001024"
     CDS             41944..42621
                     /gene="srtB"
                     /locus_tag="KPK24_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG2298730.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(42781..42855)
                     /locus_tag="KPK24_001025"
     tRNA            complement(42781..42855)
                     /locus_tag="KPK24_001025"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42821..42823),aa:Thr,seq:tgt)
BASE COUNT        14191 a         7364 c         4761 g        16584 t
ORIGIN      
        1 taataaatat ttttataatt agaaggaagt ggttttgtgc aaatagaaca actcgattta
       61 gaaacgagaa ataaaatata ctgctatact aaaaaaattc ttagaaaata tcaaaaaggc
      121 ataacatctg gaaaattaac agcagataaa tttgcagata atatattgtc tcaagatttt
      181 atcagtagta tacttaatga aaaaatagtc aatgagacaa actttaagat atcttataga
      241 aactatatag aaactttaat aaatattcaa aatgaaaatt tatctaactt aagaaaaaaa
      301 tctactaaaa ctgcaaaatg tttcaatatc tctcaaataa cccaattaaa aaacttacta
      361 agtaatactg gttataattt attaattcca tataaatact taactgctag agatatagaa
      421 ggaatagtta ctcttattaa tactgggtct attgaacttg gaaatgagag aatctataat
      481 tatatatcaa aagcataata atttaaacct attataaaaa ggcaatttat ttaataaaaa
      541 atatttttaa tattaaataa attgcctttt taattttgga ttaaatactg ctatccaagt
      601 gctttttcta gtttagataa atgttgtatt ggataatatt ttagtagctc tttaaattgt
      661 tctactgata aaccttctgt tgttgtatat attcttattt tttcatctaa gtcactatta
      721 ataaatttgt tttttaaatc ctcaaaattt acactactca ttttacatca ctccttatgt
      781 aacatattca tttgtattat tttatccaaa tacataaaat ttattacata aaaatatatc
      841 tatttaataa tttaataaaa aaaagctatc tcaatttaga aattactttc tatatttgag
      901 atagctctag ctaaactaat agccaatagt tataaataat tatttataac tatctgttgc
      961 tttttccagc catttgttgt tcagccattt caactaattt tttagtcatg tatccaccta
     1021 cataaccatt ttctcttgca gtaaggtttc ctttatcaac ttgttgataa ttactcatac
     1081 caagttcatt agctatttct aatttcattt ggtttaaagc ctcttttgct cctggtacta
     1141 ctgttctgtt gttgttattg ttacttgcca tgttgattac cttccttctg tttaatttat
     1201 tttgtgttgc tgataatatt atgtgatttt aaacattttt aattcaggta atttttagca
     1261 attaaaacct gccaaatatc cagtagaaaa agctatttgc aaattgaatc cacctgtata
     1321 tgcatctata tctattactt ctccagcaaa aaataaacct tctactaatt ttgattccat
     1381 tgtacttgaa tttatttcgt ttacttttac accaccagaa gttattatag cttcttcaat
     1441 aggtctgtat ctctttactg taaactttaa attttttaat aaatggacta agttttttct
     1501 ctgctctcta gaaatttggt gaacaaccgt atttggatta atttcactta agtttattat
     1561 tataggtatt agtttttttg gaagtaaatc atctaatgct ttttcaaatt ttttatttgt
     1621 atacttctga aaatcttttt gaactctttt atccaatttt tcctcatcta atgcaggttt
     1681 taaatctaat aatatagtgt aattttcttt ccttgtatct ttcattctac aacttgcaga
     1741 cttgattata ggtccatcta aaccaaattt agtaaattca agttctccaa aatcactata
     1801 aactttcttt tgtttgctat taaacaccct tatttcaaca tttctcaatg ataatttttc
     1861 taaatctttt gtaaaacttt cttgtacttc tataccaatt aaagatggtt ttgtatctat
     1921 aattgtgtgc ccctgtgaaa ttgcaaattt atatccatca ccagtagaac cagtaagtgg
     1981 ataacttaat ccaccagttg ctactacaac tgaatcacac ttaatttctt ttttatcgtt
     2041 taaaattact ttttcaattt tattattttt agatattatt ttttctactt tcgaatttaa
     2101 taatatattt actttcttac ttttaagttg tctttcaaga gcatttacaa tatcaaatgc
     2161 tttatcactc tctggaaaaa ccctttttcc tctttctgtt ttagtcttta ctccaagatt
     2221 attaaacata cttattacat catcatttgt aaaagtataa aaagcactat aaagaaattt
     2281 tccatttgta ggtacatttt ctattaattc ctctatttca caagcgttag taatattaca
     2341 tctacctttt cctgttattg caagtttttt tcctaattta tgatttttct caattaatgt
     2401 tacatcatag cctttctcac atgcagttga tgcggctatc atccctgctg gtccaccacc
     2461 tattattact accttcttca aataatcact cttttctacc cttaattttt ttaatttaaa
     2521 ctattgactc tataccttaa aatcaattta attttaaaag tttaaataat gctggagctc
     2581 ctgctattcc ataaaaaaca actactgtat tcttaataaa attcataaaa attatactca
     2641 tagtagagtc acttatattt aaagtgacta ttaacattga aataaacaac ttaatgccaa
     2701 tatataacat tcctattgtt actatcccaa ccaaaaacct acatattctt gaaaaaatta
     2761 tatttttact ccccttattt ttattactaa aatttatttt tttgttatta ttatctgtat
     2821 taaatttaac aaatctatcc tcaaccatat atccaaatac aaatcctact attataccaa
     2881 atttttcaaa atattcaggg ctatttagaa atatagccac tattataaat ggtataagta
     2941 aaactacaag taaaatataa tttttattat catcaatata gtcaaatatt tttacaaata
     3001 caactgtaaa aaatattcct aatatccaac ccgctatcac atctattggc caatgaacag
     3061 ctaaatacag cctagacaat cctactccta aaaccataac tgcacctatt aaatgaagat
     3121 atctcttttt aaatatagtc ataatactaa cccaaaatgt tgttgcagtt tgagtatgag
     3181 cacttggaaa agaataacca ccagcagtag aaactctcaa tgattcaaga ccttttattc
     3241 ctattggtct gggagctttt acaaactctt tgatgcctaa atttacaaca aaatttccaa
     3301 ttatagcaaa cagtattttc tgtccacatt ttttatttat acaccagtat ataattgttg
     3361 taattaatat taataacggt gcttctgtgc ttattgtaaa gacaagaaat ataaagttca
     3421 aaattggatt tcttaaattt tggaaaaata ataatatatt taactgaata tccatataga
     3481 tgtctccttt actaaaattt tatataatta actaaaaagc actaactccc ttagaaatta
     3541 gtgcttttga ttcacattta aatctattct acatcctcaa ctatacctat ataaggtaaa
     3601 tttctatact tttcagcata gtctattcca taaccaacaa taaatttgtc ttctataaca
     3661 aatccaacat agtctacagg tatattagct tttcttctct gaggtttgtc aagtagagta
     3721 catagtttta aagattttgg atttctagtt tttaaagctg caactagatt acttaatgtt
     3781 aaaccagaat ctattatatc ctctacaatc aacacattct ttccttcaat atctacatct
     3841 aaatctttta atattttaac tgttccagaa gactctgttg agtttccata acttgtaaca
     3901 ctcataaaat ctatatttac atccaaatct atacatctta ttaaatctga aacaaataca
     3961 ctagctcctt taagtatacc taccactagt aaatcttcgc ctttaaagtc ttcttctatc
     4021 ttttttccta attcataaac tttttctttt atttgttctt ctgtcaacat ttttcctgta
     4081 actttgtaca ttttgtatcc tcccgctttc cttctattaa cttatatatt atagcatacc
     4141 aaaatatcaa ttaaaatctt tttcatcatt tttaaaccta aattaacagc ttatccatac
     4201 ttttttgctt tttccattaa aaatttggaa taacgattcc tttgtattat aatctatatg
     4261 tacatatatt aatcagttat ttttatcgat tatataattt tatttttgga ggtactttta
     4321 tgagcacaaa ccatggagct aatttatata gtttatctag taagtatggc ttttcaaagg
     4381 aagaatttat ggattttagc tcaaatataa atccttttgg tacatcaagt ttagccaaac
     4441 aatacattgt aaacaatata gatatggtat ctatgtatcc tgaccctgat tacatagact
     4501 taaaaacttc tatatccaat tactgcaaat gttctataga taatatagta cttggcagtg
     4561 gagctacaga actaatttca tcatttatac atacaattaa ccctaaacag gctcttttac
     4621 tctctccagc ctattcagag tatgaaaaag agttatctaa aattaattgt tctatagaaa
     4681 aatactttgc aaaagaagaa gataattttc atataaattt agaaaatctt ataaaaacta
     4741 tcaatgctaa agattatgat ttggtagtaa tctgtaatcc tagtaatcca actggatttg
     4801 catttactaa agtagaagtg agagaaattt taaaaaatac agactcattt ttaatgattg
     4861 atgagactta tgtagagttt actgatacag atacatactc ttgtactcaa ttagttgatg
     4921 attattctaa tttattcgtt ataagaggta catcaaaatt tttttcaacg cctggaataa
     4981 ggcttggata tggtcttata tctaacacta atgtcaaaaa tgaaataaat aaaaatctag
     5041 atttatggaa tataaacata atagcttcta aaatgggtga aatcatgttt agtgatttag
     5101 attttatatc aaatactatt tccttaatga acactgaaag agattacctg cttaaagagc
     5161 ttaagaatat aaaaagctta gatatataca atactaaagg caatttcata ttgtgtaaaa
     5221 tcaagacaaa ggaattgact gctaaatctc ttcgagaaca attgcttcca caaaaaataa
     5281 taataagaga ttgttgttca tttgaaggtc tagatgagta tttctttaga gtatgtatat
     5341 taaaacctaa tgaaaataaa ttattaatat catctcttaa agcaattttt aaataaaaaa
     5401 atcactttat tttataattt tttataccat aaaaaatgag ctatctctat ttgagataac
     5461 tcatttttga ttattaaaaa actaagcact ttgctcttct ttatttcctt ttttactcat
     5521 tccatcattt attttatcta ctactactgc tattgcatta tctcctgata cattacatgc
     5581 tgtaccaaaa ctgtcttgag ttaagtataa agctatcatt aaacttgcaa gcccaccatc
     5641 tgatggtatt ccaaccattg gtaggaaagg aagtgcactc attatagccc cacctggtgc
     5701 tcctggtgct gcaaccattg ctatacctaa cattgcaata taagggaata atacacctat
     5761 tccatgtggc ataccattca tcattaaaac tgcagttgta aatcctgtta ttgatatcat
     5821 actacctgat aaatgtattg ttgcacaaag aggtacaaca aattctctta tttgcttact
     5881 tactccattt ttttctgcac aaccaacatt aactggtata gttgctgctg aagattgagt
     5941 tcctatagct gttaaatatc caggtacttg attttttaac attcttactg gattttttcc
     6001 tgaaaataca cctgctgctg tgaattgaca tgccatatat acaaaatgta aaggtattac
     6061 tactaagtat actttccaga atacccctaa tattgtccat acttcccctg ctatagttat
     6121 atttgcaaaa gttcctgcta tgtataaagg taataaaggt ataatcactg ttgacaacgt
     6181 tttcgttacg attccttgaa attcttggaa gaaattaaat aaagtctctc cttttccatg
     6241 atttcttaaa gctgatattc caagtcccat tacaaatgca aatacgattg cagctgttac
     6301 atctaccata ggctctaatg gaattgtgaa tattggagct aacattcctg cctctgggtc
     6361 tccaatttga cttgcaacac ttgcatcaat aaaatttggg aagattaatg tagatactgc
     6421 aaatgataat aatcctgcta ttaaggtaga accatatgct attcccgtag ttatccccaa
     6481 taactttcct gcacctgttg ctaaatcagc tatacctgct gttacaaaac ctataattat
     6541 aaatggtata acaaatttta ggaatgctga gaataaagta ctaactgtta ctaatatttg
     6601 tactattatt tctggtgcat aacttccgat taaaataccg actatgatac cgatgattag
     6661 tttgggcact aaacctattt ttttcttcat aatatttacc ctccataact tttttaaatt
     6721 aagcattctt gcacattgaa tacttagtta aatattggat atccacttat tatgataata
     6781 gagttttacc tctccgtcaa caatttaacc attttttgtt aataaaaaaa agaataacat
     6841 atataaattg ttattcttct tcatctttta tagaaaattc tacattttca attatatttg
     6901 aatgtttatc tttttttgcg ataactctat acttattaat ttcttcattc actataccta
     6961 tactccaaat taaaactata gcatcatcta taaaaccaaa tgcccctgca atcatctctg
     7021 gaattatatc aaccccagat acaaagtagg atatagtaaa aattaataca gaaattacct
     7081 ttattttatc aactatattg attcttttat ctgtcataaa atcaggtagt ttaagtaaat
     7141 caaatacaaa aaccaaacca aatttagcat ttttaaatct gaccaaaatt ttttttaaaa
     7201 tatctaaaac tatattaatg attattttta aaaactccat atttatcacc tcttataatt
     7261 ataggttatt ctacattata accgatataa ttaatttgat gaacatatta gatataaatt
     7321 ttatcaatta gtttatattt aaattataat aattcaattc ctgcactact acaattttga
     7381 atcatatcct gtggccatac tccaacttgt acttctccta tatgagcttt ttgtaagaaa
     7441 tacatacata ttcttgactg accaattcct ccaccaattg tatacggaag ttcgccattt
     7501 aaaagcatct tatgatagtc aaactctttt ctctcttcac atccagcaag tttcaattgt
     7561 ctatcaagag attcttcatc aactctaatt cccattgaag ataattctaa tgctgaatct
     7621 aaaacaggat tgtaaaataa tatatcccca ttcaattccc aatcatcata atcagggctt
     7681 cttccatcat gtttttctcc tgatattaat gtttttccta tttgagttat aaatactgct
     7741 ttcttctctt ttacaatttt atcttctctt tcctttggtg ataattctgg atacatatct
     7801 tctaactctt gtgatgttat aaaagttatt tcttctggta atatttcttt tataccttca
     7861 tacatactac atacaaaaat ttcagtatct ttaaatactc catacaatct ttttacaaca
     7921 tcttttagag tcttttcatt tctactactt ttatctataa ccaattccca atcccattgg
     7981 tctacataaa gagaatgtat attgtctaat tcttcatttt ttcttattgc attcatatct
     8041 gtatataaac cactaccaac ttcaaaccca tattttttaa gggctaatct tttccatttt
     8101 gcaagtgatt gaacaatttc tgcattttta ttcataaatg gtatatcaaa gcttacagcc
     8161 ttttctccat ttaaattatc atttgcacct gtttctggca atacaaataa cggagaagaa
     8221 actcttataa gatttaattc attacttagt ctcatttcaa aaaaatcttt taattttttt
     8281 atagctattt gtgtatcaat tacgttcaaa ctacttttat aatcctttgg tattattgtg
     8341 ctcatatcat tcactcccta tatttatata tttttccatt aaaaaaaccc ttcatcccta
     8401 tataaaaggg acgaaaggtt gcttttccgc ggtaccaccc tgattagttt gaaataaatt
     8461 caaacccagc tcacaatgta cagatattac tcgatacacc ccaatatgat aacggtttgg
     8521 ttccgactaa gcctactctt ttaaaatttc ggttagcaac tccaggatgt tcttcattat
     8581 agacttcata ttggtctcac accatcacca actcgcttta attacattct ataactactc
     8641 ttcctttcaa agtctttaag agtttaattc aataaaattt agctctctta tgttttctat
     8701 ttattcataa tagtatgata agtgattagt taaaatttgt caagtttatt atttttttat
     8761 ttttaaacac tttataaatt tttacctata ctttataaat tttcaaatat agtctttaat
     8821 ctgtccatac attcttttaa taaagactgt ggacaagcta catttattct ttcaaatcct
     8881 attccttctt ttccaaatat ataaccttca tcaaataata cattagcttt tttaaacatt
     8941 aaatcttcaa gttctacttc atttaatcca taagcagtaa aatctaacca tgctagatat
     9001 gttccttctg gataaacaag ttttactttt ggtaggtttt cttttaagta ttcatcaata
     9061 aattgtatat tattatctaa gtaatcattt aattcattaa gccagtcttc actttcatta
     9121 tatgctgcta ttgtagctac aatagcaaag ggatttggtg aagttgcaac tccaacagct
     9181 gtaacctctt cttgatattt cttttttagt tcatcattat tcactattat attagacaac
     9241 tgcattcctg ccagattaaa tgttttactt ggagctatac atgtaactat tttatccttg
     9301 tactctgggc ataatgactg taaaggagta tgtttaaatc cttttctaat taaatcagaa
     9361 tgtatctcat cagctattat ccataggtca tattttttac atatttctac aacctttttt
     9421 aattcatctt ctctccaaac tcttccaaca gggttgtgag gactacaaag tatcagtact
     9481 ttattgttgc tacacttagc tttttcttct aaatcatcat aatccatcct ataagtacca
     9541 ttctcatata tcagaggatt gtttacaaca tccctattat tgctctttat cttagcttca
     9601 aatgggtaat acactggttt ttggactatt acaccttcat tagaattagt taatattctt
     9661 actaaaatag caacagcagg tacaacacca ggactaaaaa taatatcttt tctatttatt
     9721 tcccagttaa atctcctttt ataccaatca caaactgcat taaagtattt atcactatca
     9781 tttgaactat atccaaatat tttatgttca acttttttac gtaatgcttc tattattggt
     9841 tctgcacatt caaaatccat atcagcaatc caaaaaggca aaccatcttt ttgtgctttt
     9901 gttgaacaat tagttctaaa atcccatttt acacaatctg ttcctactct atctattact
     9961 ctgtcaaaat tatacttact cattttttcc ctcctaagaa taaaattttt ataattgtta
    10021 agtctattac tagatgcact ttggtattat tttacaattc ttaatattat aataatatca
    10081 cttttttgtc caatagattt aaaatataat tacttttcca tcatattttt tcaatattcc
    10141 aaaatcagtt tcattatatc aaaataagat tgctcatatt aattattttc taattaacat
    10201 aagcaatctt tttctataac ttctataatt ataattttaa atgtagaatt attagtttgg
    10261 atgttatccc aatctctttt caaccaatct acaccatata tctacaccat attctaaagc
    10321 tttcgtatca aatgttacat ttggattatg aaatcctggt gtagcatcag ctcctaatcc
    10381 aatatatgta gttttacatt ttagtttttg tgtataataa tggaaatctt ctccaccaga
    10441 atttttaaat atacctatag atgcatcagc tcccaataca tcttttatag tttcttcagc
    10501 caagtttaca agttcttcat caaattctgc tgctggtact ccatctttgg aaattagtac
    10561 agcttctgca tttatagaac ttacagcagt ttttacagca tgtattgctt tttcttttaa
    10621 ttcttctatt atatcattac tttctgccct tatatctaaa gccataaatg cttcatctgg
    10681 tatattatta taagtttctc ccttaactga tatatttgtt acttttatag aatttggtat
    10741 agctggattt actcttattg catttattgc attcactgct aaacagacag catctactgc
    10801 atttactcct aaatgtggtt tagaaccatg acaagtgata cctttaatcg taaattccat
    10861 tatacagcta gcacaatggt acattgcagg tatcgccttt cccaacctac aatcttgtcc
    10921 aggtcttaaa tgcatcccta ccatttcttc tacatcatcc aacaaaccag aatcaattac
    10981 tcttaatgct ccatataatt tttcttcacc tggttgaaaa agtattttta acttacctct
    11041 ttttactaaa tttctctctt ttattaactt tgcagctgtt aaaagcatag aagtatgacc
    11101 atcatgacca caagcatgta tcattttctt ttctccatct atgataaatt ccaatgcatc
    11161 catatctgct cttatagcaa atactggtcc ttctttttta gaatctatag ttactataat
    11221 accagttcct gcaattcctt tattaatatt ttcaaacccc attttttcaa gctcttttaa
    11281 aacaaactct gatgtcttat attcttcaaa tcctttttct ggtatagtgt gtagataatc
    11341 atacacttta tgtacatact caatatagtc tttgttagtt tccataatct agctcctcct
    11401 taaaatttaa actttccatt tatattaaat ctttattata attacctata ctacgaaata
    11461 attcattata agcattgcaa tacaaccatt taaagttgct ataacaaaca tatgtggata
    11521 atatttagtt tttaactcag ctgttcctaa aagtctcccc ataaattgta tctgcgctcc
    11581 tgaaagcaat aacattggca atagaattgc aacatgtgct tgacttattt gacctgctgt
    11641 aaataaacct gcgattactc cagatgctcc tcccattgat ataaatattg tcattatagc
    11701 tgttgctgca acacctggaa gtccaaagat actcattaca ggtgtaaata ttgtccctat
    11761 tacatccaaa agacctgtta aattgaacac agaaattata gcaaaaccga agatgacatt
    11821 tggagccatt gaatttgtac ttatttggaa tccatttcta gctcctccaa taaacgcatc
    11881 tatcacattt ccttttttaa cttttacttc tttttttgct acctcactca tgttaagctt
    11941 cccccttgtc aaatttattt aaatataatc taagtaagtt cgccccaaca aacttcataa
    12001 ttaatattaa agcaagtggt ataaatattg gtacatctcc tataaatgga aatagtgctg
    12061 ctccagaagc aaagaaattc gtaattgcag acgcacctga aaattgaaat gcacaaaata
    12121 cagctttttg tttttcatct atatacccat catctttaag ttctttagtc atagatgcag
    12181 ctgcatctgt agactgtaaa ctagctatca aagttagtcc tgcaaatcca ggtaacccta
    12241 ataatggctt taatagtgga ctaagaagct tttgagctgc cttcattcca tctaagtggt
    12301 ctataactct aactactcca agtgcaaaca taactgatgg tattaatgtc aatgcatata
    12361 gccatccatc tcgagggcca gtacctccat cacctctaaa gtttgcagct aatgtaccta
    12421 caccatcatt aattgttccc aatgttccaa aagaacctaa tacattgtta aaatccaata
    12481 cttttaatgg tcctggtgca tctttaaata ttcctgaaaa aaataatgct cccaatatta
    12541 atgctacata cccataccat tttacctcaa ctttttctgt tgttgattct aatttactca
    12601 tataaattct ccctcctgtt taaatgaatt ttattagtat acttgtttaa taagcaaaac
    12661 ttatgccaat accttaatga taaagccttt gtatttattt ttttattgta aatttattcc
    12721 agtgattaaa tagcttataa ttacttactt ttcaagttta aaaatataat atttttttat
    12781 taattattta aaatatacat tacaatataa aaagctattt tccttatctt gcaaaaataa
    12841 ttttaaaata ggtttttata ggataaaatc ctaaaaaaac ctattattta atcaaatata
    12901 taaatttata ctactatttg ttgtataata taacttgcct tatactttaa atactttaaa
    12961 aataaatact attttgttag gagaaataaa atggataaaa agaagttaat ccttataaca
    13021 catgataaat tgaatagtga ccactacaaa gaagaattaa caaacttttt tggagacgaa
    13081 ataatcatag aaacacaaaa tatacttgat ggaataaaag aaaatttaga gggagatgta
    13141 gtgctttctc tatcaccttt aactagtaat tttttaataa aacattttaa ggaagatatt
    13201 gaaattattc atggaacgaa agcactttca aaacttggat atgaaaaaat gatgaaacta
    13261 cctccaggta caaaatcgtt acttatgaca accaataaaa cttcagcatt tgagatggct
    13321 acttatctat ataaaattgg tataaatcat atagattttg taccgacata tccagattgt
    13381 gatgaaatat atgatttaga tacagctata acaccaggtc agattagatt tataccaaaa
    13441 tacataaaaa atattgttga tttaggttgg agaaaaatct cattagatac atatatgtca
    13501 ttattagtag tattaaaact aaaaaatgag aaatttatag aaaaactata caaattgtcc
    13561 aaagagacac taagtcatga tttcttaaat acatcattag ataatatatc taaattaaaa
    13621 actatacttt acatgaccat tgatgaaata ggtgatggtc taatattttt caacaccttt
    13681 aacaaggtta cttttgtaaa taagtcttta cttaatatgt tgggactaga tgaaaaactt
    13741 ataaaatcac cttccttaat ggaatatatg ccaaatagtt tcttagataa aattacaaag
    13801 aacttaaata tcgataatat gattatatac atagacgaaa tagataaaaa attcatactt
    13861 tctaaaaaac cattttatct ttataaaaat atagagggtt gcttaattac attgaaagat
    13921 gtaaacaata tagaaatatt agaacaaaaa ataagaagtg attctgtcaa aagaggttat
    13981 gtagctaaat ataaatttaa taatattatt ggaaatagtt ctattataaa agattgtatt
    14041 aaaaaggcaa aaaaaatggc tcttacagat aatcctatat taataacagg tgaaacagga
    14101 acaggtaaag aagcctttac tcagtcaata cacaatcatt caaatagaaa aaataaacct
    14161 tttgtagcaa taaattgtgc ttcattacca agtgaattac ttgaaagtga actatttgga
    14221 tatgaagatg gttcttttac tggagcaaaa aaaggtggaa aaaaaggatt attcgaatta
    14281 gctcacactg gcactatatt tttagatgag ataggagata tgccacatga tttacaggtt
    14341 aaacttttaa gagtcttaca agaaaaagaa attagaaaaa ttggtggtac aagtattatt
    14401 ccaattgatg tgagaatatt agctgccaca aataaagatt tagagaaatt aattatagaa
    14461 aataaattta ggatggattt attttatagg ataagtatgt tcactttaga tttacctcct
    14521 cttagaaaga gattagaaga tatacctctt cttttagaat catttttaaa agaacttccc
    14581 tataaaaaca taaaactcga taagagtctt ttagaagctt taaattcata cacttggatg
    14641 gggaatatta gagaactgag aaattgtatt gaatatatgg cttacatggg ttccaattat
    14701 cttacaataa atgatttacc tcaaaacata tcttcaaaat taaataataa tcatatgtca
    14761 agtaatatgt ccatattcaa tgatttaaac caatatgaca aaaacatatg catttctatc
    14821 ctaaaatcat tgcacatgaa accaatgggg aggacaaagt taatgaaatt tatggaatat
    14881 aatgtcacag aatacgaagt aagaaatatg ctagaatatt tgacacgcaa tggatactta
    14941 atttctagta aaggaagaaa aggctcatca ataactgaga aaggcataaa aataatagaa
    15001 aataatataa tttaaaccta tagtaattca tctttacttt atcaaaattt atatataatt
    15061 ataatttttt atttttctat aaatttttta taaaataata aaacttattt atattaaaaa
    15121 cgaagctatc tcaatatagg gataagcttc tttttaattg ttaaaaagat aaaaatatat
    15181 ttgatttaat agaaaaagag tgcctcataa actaaaaagt tcattttgag acacttcctt
    15241 aatattaata aattaattgg cttaaaatta ctgattgata attataaagt acctctgtta
    15301 aaggagtaaa ttctgaaccg attccaaatc ttaattctgt aaacgcctct aaataatctg
    15361 ggtcacatac atcttcttgt gttaaatctt gtttcaccat ttcagtagca agataaattg
    15421 ctactgatgg tagaaaagca tccattggca taggatatcc accaagtcta tttgacatgc
    15481 caaaagcctt agccttttca cttatcatgt cgtttatttt atcataattt tgagcatctt
    15541 tttcagatat ttttagaccc ataacactag gatatgtttg tattggtgat ggattgcttt
    15601 gctctgcaac tatatacttt aattctaaag ccttagtcaa tataacttca tccatatact
    15661 catttacacc aaatacattt atatcttttc catatttttt aacttgtttt tcaatatctt
    15721 catttaaaaa ctgcttaact ttgtttttgt cttcttcagt atttatattt ggagtattaa
    15781 cttgtacaaa tggcaatcct atatttttgc aagtctcttt aatcatttct aatcttttgg
    15841 ctatattaac atctttcaaa tcatctgtag aggcataatg aataaaagct tttgctccca
    15901 tttcttttga acgttcagct aatacttttc ctctttcctc tgcactaacc cctagattca
    15961 catcaacaaa ttggcttaat tggtttttat catctcccat aggggcagaa atagttatta
    16021 tttctggacg cttttccttt accttttgta aagcaggtaa aagccctgct tggtcagttg
    16081 aaactacaat agcctgaact tctttatcgt cagccaactt tactatctta ttaatagcac
    16141 tgtctatatt agaagtaaaa ttctctggca atacaacatg ctttatagtt tgtttaactt
    16201 tagtttgacc atctttgtct ttatcaattt tattctcttc ctcatattct tttgccatct
    16261 cttccaccat attgtattgt actttatttt cagataatgg ttgagttacc actgcaactt
    16321 taaaatcatc tataattttc tctttatctt tcttgcctac aagtaaacta caacctgtaa
    16381 gtccaataat taaacatatg gatagcataa atgctgttat ttttctaagc attaattaaa
    16441 acctcctaaa tttgtacatt cttatcacta ttactaattt tatcatactt caaagaaaaa
    16501 ttatattata aaaaattata tacatgctat tataatccaa tctaaaaagt actatgggta
    16561 gattaggttt agattaaacc tacccatagt actaaaaatt aatatattat aaaattaaat
    16621 taattacaaa ttattatgct acttgtttgt tgctttttat aaagttacca actattccac
    16681 ccaatatagc tggtattacc cagttaaagc ccaaacctgc aagtggcaag ctttgtataa
    16741 atggaacagc tcctgtcatt ccatttatag ctgataataa actaatcact aatgtaacat
    16801 atgctgcacc tttaaaagca ttatcattct ttatatgttt accagccaac tccataacaa
    16861 ctattgctaa taaaacagga tatatagttt gtaggattgg aactgatatg gctattatag
    16921 aatcaacacc taaatttgat ataagcatac tagctacaca tatacctata accagttgtt
    16981 tataactcgc cttacttatt ccttcaaaat aagttgatat tgatgctgtt aaacctgtag
    17041 aagtagtcag acaggcaagt gccacaatta cacctaaaat tacctttcct acttgaccat
    17101 atgacaatat actagttatt tcaactaata aagatgcttg aggtatacta gtatcatata
    17161 aagttgaaac tgttgcacct aaataagtaa gaccaccata tactactgct agaaatacac
    17221 aggctacaaa accagcttta atagtcattc ctatttggtc tttatctgat gtatatcctt
    17281 tagctattat agacatcaac attatagttg ccatagatgc tgcacctaga gcgtccattg
    17341 tttgataacc ttgtgatata ccttcaccaa atagattttt atctatcata tgctcaggac
    17401 tcataggtcc tgctggagaa attataccct ttactattaa aacaaataat actaataata
    17461 acgctggagt taaatactta cctattatgt ctataacctt agaagaccta atagtaaatg
    17521 ctaaagttag cccaaagaag attattgaaa atacagcttt agctactgga ccatggtctc
    17581 caaatatagg cataataccc atttcatatg ttgttgcgca agttcttggt attacaagaa
    17641 gtgctaaaca tgctagagca gctacactca ttactcttgc taacccttta ccagctctcc
    17701 taagtacatc atccatgctt cccttacatc ttgctgttgc tataataaca agcattgcta
    17761 taccagcatc tgcaacaaca aatccaataa gaggaattaa ccagtctttg ccggattcca
    17821 tccctaaaaa tggaggaaac ataagatttc ctgcaccaaa gaacatagaa aataaagcaa
    17881 aaccaactat aaaaatatct ctcatctgct tactcacact aaattacccc ctttgaaaat
    17941 attttgaata ttaagataat cttactttat tttcagaaaa atgtcaatct ggtaaaaatt
    18001 cacacattgt taacgttttc ccaatttatt ttatgaatat ttttttcatt ttatgataat
    18061 tcccatcaat ttcgttaaat caattgcttg tgacacatca aaaattgctc ctctaatatc
    18121 atctatacca atctcaattc cctcaatatt attagtagta aaatctatgc cagaaagttt
    18181 agttccatta aagtttgctt tttttaaatt tgtatactca aatactagat tgtcaccttt
    18241 tacttcttga aatacagaat tttcaaaatt tgaatctaag atagaaacta tcttaaattt
    18301 agagaatgaa aagttagaat acttacctaa aacatccttg aacaaaacgt ttttaagtat
    18361 acaatcatca aatctaactc ctatcaattt actattttta aattcaactc tgtatatact
    18421 tcctccatca aaaaatgtat ttgataaatt gcagttttca aataccacat caagtaaatc
    18481 aatctttttt aaattacaat tttcaaaaga cacatttcta aatatacaag attcaaagct
    18541 aactcttaca gaatctaaat cacttataac ttcattttct ataattttat taaatatttt
    18601 ttcatcatta aatatatctt caagtatatc ttcaagattt tcaataattt ctaattctcc
    18661 tgtaaattta ggtttttgca ctttaataat tttagtcatt ttcccctcca ctataaatta
    18721 tattattttt gatattattt tttttaatat attaacttaa tatacatatt tgatatatta
    18781 aaaagttttt acgaaatatc catgtattca gtacttttat aattatatca caaactcatt
    18841 tataataaac ttccattaat agtataaaac aagttagaat aattgacatt atattcatac
    18901 atattcaatt attggaaaaa gtaatttttt tcaataaata accttaattt tttataaaat
    18961 cgacaaaaaa acgaataatt ttattatatt tattgtaaat aatacaatat tacgatataa
    19021 tacaacagaa aaacaaatag aaaatgttca tataatatta gtaatacggt ctaatagttt
    19081 ctacaaactc accttaaatg ggttttctat gaatagaatg tcaactctga atatatgttc
    19141 tattctatct atatgaatat atattttttt gtttatatct taaagttttt tggaggtaaa
    19201 aatggtacca aacactaaca atgtacaacc aggtggaaat aattcatcat taagcttttt
    19261 agacaaaatg tttaaattta gcgaacgtgg ctctagcctt aaaacagagg taataggtgg
    19321 agttactaca tttttaacaa tggcatacat agttttcgta aatccagcaa tattatctga
    19381 agctggaatg gataaagccg cccttataac agttaccata ctagctacag ccattggtac
    19441 acttatattt gcgattttag gaaatgcacc atttgctcta gctcctggta tgggattaaa
    19501 tgcattcttc acatactctt tggttatagg tgcaggaatc ccatggcaaa caggtcttgg
    19561 ggtagttttt ctatctggat gtgtcttctt tacactcgca gtaacaggtc ttcgaaagac
    19621 aattgcagac gcaattccta gagaattggc agtagcatct tcagcaggta taggtctttt
    19681 ccttgctttt gttggtttaa aaaatatggg tgttataata gctaatccag atactatagt
    19741 ttcacttgca gattttacac caacagtttt aatagctctt tttgctctta tcttaatggc
    19801 aatactagaa tataaaaaag ttaaaggtgg aatattgata agtatagtag ttgcaacaat
    19861 aatatctgtt atatttggat ttgtggagtt gccaacagca ataatctcga cacctcctag
    19921 tatagcacca gttgctttta aattagatat tgtaggtgca ttgaaattct cattaattgg
    19981 acctatattc tctttcatgt ttatagacat gtttgattca ttagcatttc ttattacttg
    20041 ttgtaaacaa atgggtcttg aagatgagaa tggtgaaata aaagatttag gaagaatgtt
    20101 atatgcagat gtttcttcta ctttaatagg ctcattctta ggaactagta ctgttacaac
    20161 atttggagaa tctgcttctg gtattgcagc aggtgcaaga acaggacttg cttcaatagt
    20221 aacagcagca ttattcttag ctacacttat atttacacca ttacttggta tagttcctac
    20281 ttacgctgca gctcctgcac ttgtaatggt tggtgtattc atgtttgaaa gcataaaaca
    20341 gattgatttt gctgatagca aaatagctgt acctgctttt gttacagttt tattgatgcc
    20401 tttaacttat agtataagta ttggtttatg ctttggattt atttcatata taataatgca
    20461 tgtagtagct aaagaaactg ataaaatcag tattacactt tggattatag gtttacttgc
    20521 agttgtaaac ttagttctaa gttaatattt ttaaatagtt ctaggtcaat attttaaatt
    20581 atatagaata aaatcttatt tattttaaat ttataaatag caaaatcact ttatatgtaa
    20641 attgattttg ctattaactt tgtatagaaa atttattatc ataatatata gatataaaaa
    20701 tatatagaaa aggagtggtg tttatgtcac aattacttat aaaaaatgca tatctaatta
    20761 ctatgaatgc agatagagag gtatatgaaa atggaagcat acttattgaa aataatatta
    20821 taaaagaggt tggtaagttt gatattaata ttgtagataa agatgcagag atatatgatg
    20881 ctaaaggaaa aatactaatg ccagggctag tcaatactca tgttcactta tctcaacaat
    20941 taggacgtgg tcttgcagat gatgttgtac ttttaacttg gcttagagaa agaatttggc
    21001 catacgaaag tagcttcaat tatgaagatt ccttgatttc atctactgca tgttgtgttg
    21061 aattgataaa atctggtgtc actacatttt tagaatctgg tggtcaatat gtagatgcta
    21121 tggttgaagc tgttgataaa actggactta gagcctgttt agctaaatca gttatggata
    21181 caggagatgg tcttcctgag gcatggcaaa aaactactga tgaggaaatc aacactcaat
    21241 tagacttatt ccataagttt aataatacat ctaatgagag aattaaaata tggtttgcat
    21301 taagaactat ttttaataca actgaagaat tacttgttag aactaaagaa ctagctgata
    21361 aatacaatac aggtatacat atgcatattg ctgaaatagc tgatgaaatt tcatttgtaa
    21421 agcaaaacaa tggagttgga acagttgaat accttgataa actaggtgta ttaggaccaa
    21481 atcttttagc tgctcataca gtttggctta ctcataagga aattgattta tttagattac
    21541 atgatgtaaa agtttctcat gacccaggtt cagctatgaa agtggttcta gggtttgcat
    21601 caattcctga aatgttagaa aaaggaatac ctgtttcaat tggtactgat ggagctcctt
    21661 caaataatag aatggacctt atgagggaca tgtatcttac atcactaata cataaaggaa
    21721 gaactttaaa cccaactgtc gtaccagctg aagaagtctt agaaatggct actataaatg
    21781 gagcaaaatg tgctttacta gaaaaagaaa ttggtagtct tgaagtagga aaaaaagctg
    21841 atatgattat tctaaatcca gatacaattc attgtttacc aatgcataat ccaattggaa
    21901 atatagttta ttctatgact agtgaaaatg tagactctac tatttgtgat ggtaaatggt
    21961 tgatgaagga aagaaaagta ttagttgtag atgaatctga attattaaat aaagttaaaa
    22021 aacaagcttc taaaataaaa gataaagcag aagttaaaat tccatctaaa tttaaaatta
    22081 taaaataaca taaatttcaa ttgttttgta aatatgaata tataaatata tttatataca
    22141 tcatatatac aaaacaattg tttttttatt ttatattcat tagcattgct cataaaatac
    22201 accataattc cagaaaaaat aaccaatcag ttaggattac atcatctaag caatatataa
    22261 acgtcttagt ttataagttt tattctactc ttatctaaaa ccatctttaa agagttttca
    22321 actgtttcaa ttacatcttt ataactcaag tacacatttg cgtagataca atcttcatat
    22381 aaattatatt cagttactct acaatacagc agttttcctc catctaataa attgctcaaa
    22441 atatagtttt cttttcttgg gacatagcct atcacatatc cagtttcacc aactatttta
    22501 atagcttttt catcatatag attatctttc tctctagtta aatagtatat cttacttttc
    22561 tccaattctt tttcaagata ttctctattt ctaaaccttg aaccagcaac agctattgaa
    22621 gtcaaataga tatccttacc atgttcatct acttttattt tttcaatatt tacaaattct
    22681 ttttttaaat tattactttc atataatact cttcttaatt catccttcaa tataacattt
    22741 ttttcttttt catacgcttc tttaacaatt tttaattctt caagagatag tttctctctg
    22801 tatcttttta agatatttat ggattctttt cttacttcat ttatatttcc aaatattcct
    22861 tttaaagata tattctttcc ttcaggatat agtgatttac ttcctttaag cacaataaaa
    22921 aataacatat catcataata ttcttgagtc aatttatctt tttctatatc tttcattttt
    22981 ccaattaaat catctatttt aaacgtctct tcaaaaaaat ttaataattt taatctagaa
    23041 cttgtcgttc cctttttaga tatatgccaa tatacctcaa aatctctaat atctctattt
    23101 agatatggca acaagtcatc aaaagataag tttacatcat aaatttctga catttttata
    23161 atatcttctg tttttccact agcatctctt aatgcttcaa aatatatatt tttccatttt
    23221 tcactgagca agataccatt tatttctttt tcaaattcaa tcttattgca ctctatattt
    23281 tcatctttta agacgtttat agctattagg aaaattgaat ataaactttc aaagttagtc
    23341 ccataattta ctacagtagg taaaaatcga ttcactaatt ctaatttatt acctatatat
    23401 ttaaattcta cacttagcaa gtaattgcat ataagacgtg ctaaattatt tatcttttct
    23461 ttatctaaat ctcgtttttc aagatattca ttcaaatcta caattgaaat aatatagttc
    23521 atcaataatc gttcgtactt tgtatcctta taaccatctt caattagata ggaatttatt
    23581 ttagaatcta aattttccat attaacaata gcaaatactt ttattgaacc tgtagccttt
    23641 ttagataaat taaataaata tgtattgtaa aattcaagtt ttttaatagt attactcaga
    23701 taaaatacgt attctccaga tttagaaaat gtatctacaa cttctcttaa gttttctaca
    23761 tttagatatt tctcacttaa aactaaccct agttttacat actcaacttt tttactctta
    23821 tatattaatt ctttagttaa tttatataac catttctcat caataatcat ttcttttacc
    23881 aaagtattta tctttttcat aaaaatttcg aaataacaaa ttaaagactt agaattacta
    23941 ccctccaata aataaatttc attagattca cttttataca tatttaaaat ttcatctact
    24001 agattatttg caagctctac ttgttctctt aacggtatac tttcatatat acataggtaa
    24061 ttcatttcaa atgattcttt gtatccatca taaccattaa attcctgcaa tcgcttaaaa
    24121 acattctcca ccaaattatc ctcctttaca tgatgtaatt tatattgatt atactactta
    24181 tttcatcaag tttacattta tacgcaaaaa aatattacta gaaattgaaa ttctagtaat
    24241 atccttatta ttaattatgt aaatacccta tcttataact gtaaataata tccttattaa
    24301 ttttaattac ttttaataaa aatctatcct agatttagac ttctctatgc atatattata
    24361 ctaatattag acatcataat catacacttt ttctttttca tatctcatgt ttacaaacaa
    24421 tgcaagtaaa attccaacaa atgttattgc cacattaaac aatacaacat attttggtcc
    24481 attaactccc cctgattttg taataatacc tgctatattt aaaataacat agtttgctat
    24541 actagatgca atcataacta tagatgttat ttttccttta ttagttggaa acatttcatt
    24601 tgcagttgac gttgttaact gaagaacacc tcctgctgct gaataaccta ttacaaatcc
    24661 ccctaacata caaatagttg gagtttgaac aaaatataca actaatagca taagtaaagc
    24721 tattatagga tatataacta atattcttat tggtttaata tattttttaa taagtaaaga
    24781 agttattaat actgcacata ttactcccat tgagtaaaag gattgaattt ttgtagggtc
    24841 agccatccca tacaatttac ccaattcctg attacaattt aaccataata caaatgtaga
    24901 tgtacatgta aatccaatac aaacaagagc tatacttgtt ggtgtaaatt tcatcttctc
    24961 tgatttaact gttttgcctt tgttatcaat aacattatta gctggtggaa atggtaacac
    25021 agctatcaaa aaggcatcta ctacaataag aatcgctgtt actataaaaa taactttaaa
    25081 tgatattgaa tttgctgcaa caaatccaat tataaaagga agcaaaaatt gtgctaaagc
    25141 tattgtaaat ttagtaaaca tatttgctat tgttcctaaa ctagcaaata tctccataca
    25201 agaaggagtt acacaggtat ctaagaatga atttgctgcc cctccaatta ttgcaaatac
    25261 atatgctata taaaaatttg gtgaaaatac aattccaaca aagaaaattg catgtagaaa
    25321 gtttcctata agaccactta cttttcttcc atacttatct gaaataggtc ctgcaaatgg
    25381 ataagatata agtctcccta atcctagtgc agcaataaca gctaaaacaa tacttacatc
    25441 caatgttcca tctgataatg ttttagcccc ccacattgat gaaaattctg gtttgtattg
    25501 tcctaaaatt gaaattccaa taccaagtaa aaaatatgaa aaatacaacg ctatggcagt
    25561 aggataatat tttttattgt tcataatttt tctcccattc ttgttaattt attaacattt
    25621 ttgtgtacaa taaactcatc cccataatgg atttaaagta tagtcagtta aaacttcaaa
    25681 tttaactgga ctatgaggca aaacccacta tgacaatttg taatttatta ttaaaaatat
    25741 tgaaatacgt atgttttttc attgtttaag atattactct tatctattta aagcataatt
    25801 cattaacttc ttctacaggc atttctaatc cagtatatag attaaaagct tctgctcctt
    25861 ggtataataa cattcctaat ccaccaatag ccttacatcc attagcttca gcatcttcaa
    25921 tcatttttgt tttcttagga ttataaacga catctgttac tactaaatct tttcttaaaa
    25981 cactagtatc ttttatattt gtttcattgt cataaggctt cataccaata agagttgcat
    26041 ttgtcaatat atcacttgat tgtatctcct cataaagttt gtttgtatct tctaaatcat
    26101 atagattaac aacacattct ggtacatctt tttttatatt ctcaactgtt tgctctgctc
    26161 ttttataaaa atcatcctta ggattaaaga tagatatttc tcttgctcca tctaaagcac
    26221 attgtacttg tatagcagtt gctgcaccac cagcacccat aattgtaatc tttttacctt
    26281 taacttctac cccattttct tttaaattac gaacatagcc tactccatct gttatatgac
    26341 caactaactt tccattgtca tttacaattg tattacatgc tccaattata cgagctgctg
    26401 gagaaagttc atccatatat tctgtaacag cagatttaca tggcatagta acattggctc
    26461 cttttatatt aaaagttttt attgctaaaa gtgcctcttt taccttatct actgtgatat
    26521 caaaggctaa atatctataa tctaaatcta atttcttaaa actataatta tacatcactg
    26581 gtgatttaga gtgaccaacc ggtgtaccta ttaatgcaaa taatcctgtt ctacctgaaa
    26641 tttccataac tataatctcc tctcaatatt atcattttct ttgcaaataa cctattcagt
    26701 tatttacaaa gaaaaattta tttattattt atatatttaa tgctacttca aaagcttctt
    26761 ttgttgcatc taatgcccta cgagctttta ctgcatcacc aacaatataa gtttctttta
    26821 caacttcttt tataacttca cttaatgggt cgtaattacg agaacccata gcaagtacta
    26881 ctgaatcaaa accttttaaa tcatgctctg tgccatctgt aagtaaatag tcgacaccat
    26941 catcataaaa tttagaaact tttgcaccag taatggtatg tatattataa ttttcaaaat
    27001 cattcataag gaatttacgg tgttctgaaa ttacatctgc accaacttct tctctaagct
    27061 caataacagt aacctcatgt tcttgttctc caaggaaggc tgccacttca catccaacca
    27121 ttccacctcc aacaacaagt acttttttcc cacaagattt tttaccatca agaaggtcta
    27181 cagcatgaat taatcctgaa tcattgattc ctggtatagg aagtacaagt ggagttgctc
    27241 cagtagctat aataactaca tcaggtgatt tctcctttat aagttctact gtagcttctg
    27301 tattagtaca tatttttaca ccatattgat tacattttga aatataactt ctaactaagt
    27361 tagtaatatc tcctttccct ggaggatatg cagctaaacg catttgccca ccaagaactt
    27421 cagatttttc aactaaagtt acattatgtc ctcttttagc acatacccat gctgcaagca
    27481 tacctccaac acctccacca acaacaagaa cttctttgct aatttcagct ggcttaagct
    27541 ctgcttcatg tcctaatata ggatttgcaa gacaggctat aggtttgcct tggaacatat
    27601 ttggtacaca tccttgtaag catgcaatac atggtatcat ttcgtctagt tttccatttt
    27661 tagctttatt aggcatttct gggtcagcca agctttgtct accgaatgct actaaatcac
    27721 atcttccttg tctgaccatt aactcagcaa actgtggctc tgtataacgt ccaactgtta
    27781 ttactggtat actaacagct ttttttattt cagtaactaa ttctgcactg aatccagcat
    27841 gtaaagtcgt aggagcccac atatattcat catgtatatg tatactacgt gaaacatgaa
    27901 gtccatctat tccacaatct tctagatatg ctgctattac tgaactatcg tgtacatcaa
    27961 ttcctcctgg aacatcatca gtgctgttta tacgacataa tattgctaga gaatttccta
    28021 cttttttacg tatattttca atgataagac gaggtaatct cattctattt tcaaaacatc
    28081 caccaaactc atctacacgc ttatttgttc tttgtgatat aaaactacta accagatatc
    28141 catgagcgca gtgaacttca acagcatctg caccagcttt ttgtgcacgt aatgcagcat
    28201 ctccataaga ttctataagt tcatatattt cttctgttgt tattgctagt ggtgtattac
    28261 gaccatttga tgcaggtatt gcagaagcag cttttaatgg atgtcctgct actttttcat
    28321 ttccttcaga accagcatgt tgtaattgta tagatacctt cgaacctgca tcatgacaag
    28381 catctattac atttttaaaa ctatctattg tgctatcatc atataaacaa ggcttacgag
    28441 gacctccttt tgcttttttg tctacaacac tagattctat agttattaat ccaaaaccac
    28501 ctaaagcacg ctcaagataa taagccttag aagtttcact taaagtacca tctgtgtttg
    28561 caaaattgtt tcccataggg ggaactacaa aacgatttgg aactgtcatt gaaccaattc
    28621 ttataggtgt aaacatagaa ctaatcttca taaggttttc cttcctttca agttttatat
    28681 tataatactc tgtatatttt tatattatat aagtttattt tggagatact tcagaccatg
    28741 ctttatccaa aactttttta gttgcattaa atacagtttc agcagcagtg gataaccctt
    28801 tagatattag agaatcagat attacttctg cccctatgac tcttggtgat atattgtgtt
    28861 cttttattat tttcacaaat tcctctgtgt ttccatatcc ttcacctgga gccaaacggt
    28921 catgtagtga ttcatcccta agtttttcat aaggagtttc taaaacatca cacacttgaa
    28981 ttgatactat cttatctgct ggaacaggtg caaggtcctc tgctgtctgc tttgctctag
    29041 cccagtgcca agtatctaat ataagcattg cattttcacg attacatcct ttcacaacac
    29101 gccatgcagt agccaaatct ggtacaccac tatatggcat aaactcaaga cctataataa
    29161 gttcacctgc tctgtcacat aattctccca atgcagttat aatttgttct tctggtatca
    29221 tctctaataa tccacaattt atatgtttaa cattaaataa gtgagccata tgataaacat
    29281 tttgctcttt ttctctttga gcttccgttc tatcttcttc tcttccccat tgagttatat
    29341 attctacttc tgtaactttc atatcatatt cattaagaat tgcaatcatt tcttcatctg
    29401 taacacctgc attttttgca tcaatataat tttcagcacg caatccaata ccatcaaacc
    29461 cagcttcttt tgcaatacga actctatcac ggaatgaaac ttctgttccc aatgtataag
    29521 aactaattgt aattggaata ttaagatttt tttgattatt catagcttaa atttgccccc
    29581 tttattttct ttataattaa tagtattttt gattttatat ttttatttat cctactagta
    29641 gtattatttt accttaaaaa taaaagtatt ttttgatttc tatttatcta gattttataa
    29701 atttattgta tcacgagact ttactaataa caaattgata atttcctatt aattcataga
    29761 ttttgtttat agattttatt tataagataa ttcttgtatt tttctattag gtcgtataaa
    29821 tatatttatc ttataattca gacaaaaaac gataaatttt tttaaaagct tttattctta
    29881 ctctataatc atatataata aaaatgtaaa aatatcttaa aggttaactt ctacaaatat
    29941 ataaataaaa atacctatga aaggatgaac aaaaattgaa tttatatcac ttacgctatt
    30001 ttgttacatt ggcacattta gaacactata caaaagcggc agaaaattta tctataacac
    30061 aaccaagctt aagtcatgct atttctttac tagaaaatga acttggggtg gctctatttg
    30121 aaaaagaagg tagaaatata gtccttacta agtatggaaa aatattttta aaagatgtgg
    30181 aaaaatcatt agaaatttta gattcaagtg taaaatcatt aaaaataact ggtactggtg
    30241 aaggacaaat agacttagct tttcttagaa cacttggtac tgattttatc ccagacatag
    30301 tgcacaaatt tttaaaatct aatcctgcta aatcaataga ttttaaattt catactggtg
    30361 taactactga cattattcaa ggtcttaaag aaagaaaata tgatattgca ttttgttcga
    30421 aacttgaaaa agaaaaggga attgaattta tacctgttgc caaacaagat ttagtactaa
    30481 ttgtaccata tagtcatcca ctagctgcaa aggatactat agatttaaaa gaaaccatac
    30541 cttatcctca aatagtattt aatcagagaa gtggtcttag atatataatt gatgatatgt
    30601 ttaaaaaaat aaatcaacag cctaatatag tctatgaagt agaggaagac caagtaattg
    30661 ctggattagt tgcaaaaaat ttcggaattg ctgtagtccc aaatatgaat atgcttagct
    30721 ttacaaaagt aaaagtaatt cagattattc acccaagttg ggaaagaaac ttttacttag
    30781 catttataaa agatagatat ttgcctcctg caattaaaaa ctttaaaaac tttgtcatta
    30841 aaaacgctca attataattt attaataaag gggttatttt taaatatcct cttttattct
    30901 ttttataaaa taaaaaaatc tatactttta aaagtataga ttcctaactg acagtcggca
    30961 tccgagtctc agtaattatc gttatacaac agcaactgga ttcagttcct gaataactag
    31021 gttacttaca ttatatcaga ttttttaaaa tttgtcaatt gtatctaacg cattttttca
    31081 agccatggtc caacaggctc tacaatagga acttctttaa atccccaatt tcttatatca
    31141 ttcacactcg ttggagtata tattttgttt ctgacagatt tgaatatttt gtttttttct
    31201 ccatattgca tacactttgg attagcttct attaaccctc ttcttgctct gctaagagga
    31261 ttacataaaa tatctgctat ttctaaacct tctctatagt cagcatcttc ttttttggca
    31321 attataaaac ccttaacctt atctcttaat tcttcatcta tatctaagta tattgttcca
    31381 ttattaaata catcaaaaaa cgccttttgt acttttaagt ttgagacatc atcccttgac
    31441 tctataacta ttcgtgcaga ttctacagtt tcattattta caaatgaata aaaattctga
    31501 agaacatgta taaaagccac aacatacggt tcctttgaag gtgcataata tttctttaat
    31561 ctagctttat ctactgttac tgatattata gtacactcta atctagatag aaataaaggc
    31621 aactcatcat agaataactt aatctgtgaa tcatcaaact tctcaaatgc tcttgatttt
    31681 cttgatatat ctgtcaaatg tattcctatg ttactggttc caaaacaata ctctttaaat
    31741 gcatccattt ctttcctcaa agaacataca taatcgcctt gacttactgc atatttatat
    31801 tcaaaaatta ctcctgtcaa tgtaaatggg tcttcatgat ttatatcagc tcttccactt
    31861 tcatcaataa acattatata ttttttcttt ataatcttac tcaccctctt aacatatgat
    31921 gatagcatct atattcattt tatatgagtt ttaaaaatat tccaagattt ttatataact
    31981 taaacttaat tttatactaa aaaattgaga tgtaaaacaa tcaagctttt attgattgaa
    32041 tttcacatct caattataat tatttttcaa tatatatttt tactattttg caagaatatc
    32101 cattaattta tccattacta tataagatac tccgcctcct atttgataag gagtttcaac
    32161 ttttttattt gcaaatactc tttgttgctc tgatgataat cctgaattat ccattaaaac
    32221 tattggtgaa tgtaatttta cagctaatgg tccagctgat aaagcatcta ctaaagcatc
    32281 tgatttacta actaatacag cctgtaaatc agtatctcca tagaattttt ctatcacttt
    32341 ggcattagta tctactctac tatctccata tattctattt cctgaaatat cttcagtagt
    32401 tatatcattc atttttgcta ttactgaatc gttaattgca cttggtccac ctataaagta
    32461 tgcattttct aattttcttt ctgttagcca tttataagat tcttctgtaa ttccatcttt
    32521 attgactaat attataggtt gtttttcttc cccagcttta gaagctacag ataaagcatc
    32581 tgcctcccca tttccagaaa ccatatatgc agtttttatt tcttctcgat ttccaagttc
    32641 ttcagctatt cttgtagcag tttgatatct atcttgacct gctattctct ttgtttctgt
    32701 gttgaaagta gtttttattt cttgctctaa tttagaactt attgcatttt ctccacctat
    32761 caagattact gttttaggtg ctaataactt catttcttca acagtttcag ttggtaaatt
    32821 agctgtatta gtaagtagta ttgttgagtc atagctactt gctaaaggag ttgctgttat
    32881 accatcagct attgcattac catttactaa tattacagta tctgaactat cagcattcca
    32941 gtttccaact gaacttatct ttttagctgt atcaaatcta tttgcaccta taattgtatc
    33001 ccatgtagga tttatagttc catcttcatt aaaagtataa tctttcccat ctattttttg
    33061 agaaccagtt agcatctttc catctttgtc taaatagtaa gacttaccat ctatttgttg
    33121 ccatcctgtc tgcattattc ctgttttgtc aaagtaatac ttattaccat ctatttcttg
    33181 ccatcctgtt actgctaaac catcagatcc tagatagtat ttctttccat tcttttcttg
    33241 ccatcctgtt tgcattactc catctgagtt aaagtaataa gtttttccgt ctattacttt
    33301 ccatccagat actcttgctc catcagttcc taaataatat tttttgttat taattgtttt
    33361 ccatcctgtt tgcattactc ctgaagcatc aaagtaatac atttttccat ttacttttag
    33421 ccaactattc ttttggtatg ttccatcaga ttttacataa tacttcttac cattttctgt
    33481 tttccatcct gtttgattta gatttatctt atattttgcc gctatcttta catagctgtc
    33541 gttactataa tctataatta catctgaacc tacaggaact tggtcaaata accatctaac
    33601 atctttattg tgcattctta tacatccacc agaaatatgc tttcctatag aactctcatt
    33661 attatttcca tgtattccat atgtatctcc ataagttcct tttaatgcta atcccatcca
    33721 tctatctcct aatggatttc ttggacttcc tccaggtata ttacctttat aatatggtct
    33781 attttttatc ttgttaacta cttttgtttt ccctgttggt gtttctgacc cctttttacc
    33841 tgttgcaact ctaaattctt ttacaagttt attatttaca aaatatccta gagtattttt
    33901 tcttgaattt actataatca catgtttact ggctgcgctt gattctggta cgccacatgc
    33961 aaatactaaa actaaagtca gtgctagtac taaatacttt tttagacttt ttttcactat
    34021 aacttccccc tcttttagaa acattactac atgtgtctaa ttataacata aaattaccaa
    34081 taccttatat ttacaaattt gttacattta tacatataag tatgtattct actcttcata
    34141 cccattagga ttcatacttt gccatctcca tgaatcttca cacatctctt caagttcata
    34201 cttagctttc catccaagct ctttttctgc ctttgaagaa tctgcataac acatggctac
    34261 atctccagcc cttcttccta caatcttata tggtatttct tttccacttg ctttagaaaa
    34321 tgcttttact aaatcaagaa cactataccc ctttccagtt cctaaattat aaacaacaag
    34381 tcctggattt tcttctaatt tttgtaatgc ttttacatga ccagcagcta aatctaatac
    34441 atgtatatag tctctaactc cagttccatc atgtgttgga tagtcattac cataaacact
    34501 caattccttc aatttaccta ctgctatttt tgttatataa ggcattagat tgctcggaac
    34561 tccatttggt tcttcaccta ttctaccact tttatgagca ccgactggat taaagtatct
    34621 taaaagagct atatccaatg acttatctgc ttttgatata tctactaaca tctgctctat
    34681 cataagttta gttcttccat atggattggt tacagaaagt ggaaaatcct ctaatatagg
    34741 acaagtatgt gggtctccat atacagtagc tgaagaacta aatacaaact ttttaacacc
    34801 atattctctc attaattcaa ataaatttaa ggtacttatt agattattgc tgtaatattc
    34861 aattggtttt tctactgatt caccaactgc ttttaaagct gcaaagtgta taattgattc
    34921 aatattattt tctttaaata caacatgcat ttcatcttta tttctgatat caatattata
    34981 aaattttact ggttttttag ataattcctt gattctatca accactattg agttgctatt
    35041 acttaagtta tcaactataa caacttcata acctgattct aaaagttcta ttgctgtatg
    35101 gcttcctata tatcctgctc ctccagctac taaaactgcc attgtaatta cctcttttct
    35161 aatatttatt tttcacttac tttttttctc atatagtcca ttaaatcatc ttttaaattt
    35221 tctctcttta gagcaaagtc tattgttgcc tctaaaaaac caattttatc tcccacatca
    35281 tatcttctac cttcaaagtt ataagcataa atagcctctt gttttccaag agtttgaagt
    35341 gcatctgtca gttgtatctc tccaccttta ccaggtgtct gtttctctaa tatgctaaat
    35401 attgcaggag ttattatata tcttccaagt atagctatat cagatggagc ttcttcaatt
    35461 gcaggttttt caaccatatc tttaacttta tatactctat cttcgatatg ttttacatcc
    35521 aatataccat atttattagt atcttcttta gctatctttt gaacacctaa tacagttgtt
    35581 ttatactcat tataagtatc tattaattgt tttaagcacg gaacatctga atctactata
    35641 tcatcaccta ataatacggc aaaaggttca tcacctataa aactttttgc acagtaaatt
    35701 gcatgtccaa gaccttttgg ctctttttgt cttatataat gaatatttac catatttgat
    35761 atgtctctta ccatttctaa catctctgtc ttaccttttt gctctagttc taactctaat
    35821 tcaactgatt tatcaaaatg atcttctata gattttttat ttctacctgt tattataaga
    35881 atttcttcaa ttcccgattc tatagcttct tctattatat actgtaaagt tggtttatct
    35941 actataggaa gcatttcttt gggctgtgat tttgttgctg gtaaaaatct agttcctagc
    36001 cctgctgctg gtattattgc ttttttaact gtcatctgca ttttttcacc cttcttcgta
    36061 aattttctat tgtaccaaat taatatacca tatttatact ttttcttcaa actctatatt
    36121 atcccaatat ttaatgctat aagataatat aaggtagatg aaatttcatc tacctttagt
    36181 tttataagtt aatattgtat tgtttatatt ttatccaaaa cttatacagg tactaaattt
    36241 catctaagat gccttttata tcactatcta tatcttcatc tagataagaa tctaaatctt
    36301 ctaagtcaac ttctagttct tgatatgtca tatcaacact gacatcctca tctatatcca
    36361 tttctgatat taattctcct aaaatactat ttccatcaga atctaaacca ctatctatat
    36421 cttcatctat atagttttca tttaaaattt catctaaatc aatctcgtaa taaacttcat
    36481 tatttttttc atcccctgag tccaatccta agaactcata gttaaaagtc caaacattgt
    36541 atttacttga catactttat tcctcctgtg aaaaacatat tttcatctag tttattggtt
    36601 ccataatcaa cagagttata tattttttca attatgtctt ttcttctttc ttttgtacta
    36661 ttatttaatg tcaattgttt aaatacatat gtatttgtta ttctctcgaa taatatttct
    36721 tttatactat catcatcaat atttctatag tttttccaca ataactcaaa taacttaaac
    36781 atttccctac tagtttcatc tacataataa ggatacatat tttctactaa atggtttaat
    36841 cttgatatgg cttgttttaa aaatacatca agacttgcac tttcactttc ttttaacatt
    36901 ctataattgt atttcttctt atcttctcct gaaattacaa agatgatatt gtctaaactt
    36961 ttttcaactt catttaaata actgcttata ttatatgata aatcagacaa tagattttta
    37021 atctgcttaa acacttgtac atcaagttta gtattatact tttcaaacaa atctatttct
    37081 tgattttcta atattcttgg catatatgct gacaataaat ctaaattgtt tctttgttct
    37141 tgtatttgct tataaagtga atttataact tctattctta tttcatcatt taactctttt
    37201 tctaaaatct tatcaaattc agatttcata tagtaaatat ttccaaagaa attaaatatt
    37261 atcatatcat tcatagcttt agaccaattc ttatatatca cttcaataga attttcttta
    37321 agtagaggtt gctttgcata tataaataca tcttgcataa gtttttgaac catactttta
    37381 cttaagttta aaagtacttt ttgattatta aattttattt ccgtttctac aaaattagaa
    37441 ttttcgtttt caagttcttt attaattaca atgtctttta tatcactatt ttttatagat
    37501 atgatttctt tttgaaaaga ctttagaata gtcaagctat cgaaatcttc ttgaataaaa
    37561 aatggagcat gttttaatgt atatcccact atttcatttt ctatatcttt taggtcatct
    37621 atttgcataa tttctctatt tttatcattt atatgtaggt taaatacatt tttattatca
    37681 tataatatta tatcttctcc taaatttaac ttagaacaat tatctttcgc tgactttaca
    37741 atagtcttct tagatttctt atttataaaa attaattggt cttgatattt tactaaaagc
    37801 atgtttgtgt ttttataatc aaatccatct atatttcctg atatcttgca gaatactatt
    37861 tcacaatctt taggaagatt tcctattttt tggttatctt taactctctc atttaaatca
    37921 gatgggacta cactatctat attgttatta aatatagata cagaccttac tataatcccc
    37981 tctctttcca tatgaaagta tcccttcaat gacactcttt tatcttctaa aatgtatctt
    38041 tctatatcat caaaatttat ttcaaatcta tttttcccac tgataaatat aaacttctca
    38101 ttatctttat taattccacc catataaaac ttattgtcat ctaaacttaa tatagtcata
    38161 ttatccttta gatagaatat aaattctctt tgacttttgc agattaaatc atattcatcc
    38221 aaagttattt tcatagtcaa atttgctgta acatttttac ttgaattttc actttttatg
    38281 taaaaaggca agttatttcc atagacaatt atttgagaat cttctaaaac tgcctctaat
    38341 ttttctttat tgtctgaaaa actatatata tattttacac ctgtatcctc attaacttca
    38401 tctgatttgc tgattattat attccctaga tttgttatat gaagcttttt atattctttt
    38461 gagtatctac ttatgccact aagctcaaac ccatctgaaa gctcatatat gattttttct
    38521 cctaatttca acataccttc accacctaaa cagaattata agtattttat aaaggtgaat
    38581 aaaatgtctc aaaacaaata atattttttt taatactttt tattctgaga catagtttct
    38641 ccttttttat aatgctctaa gcaccttcaa ctatttcttc ataaagttta tctaaatgtt
    38701 taacatattc acgatatcca gatttgttct ttaactcact tcttaaatct tcatattttt
    38761 gtttaagttc ttcagcactc aaagaaggag ctgattcttt taattcttct aacttataat
    38821 catagtactt atcatacata gctatatatg tatcaataga cttcttagct agttcattac
    38881 atttttcttg tgcttctcca tattgagaat aatcacctat ttttatttta tagtatccat
    38941 tttcttgaat tattgttgaa gaaatacctt tagcttcata ttcttttgct gtttcttttg
    39001 ctgttttttc aactttaagt gaacctgcat aaacagtgta atatacacct ttaccatctt
    39061 tatatgcttc catttctttt gctttctttt cagcttgtgc tttttcttga gcctcttttt
    39121 cagcttttgc cttttcttct gcttcttttt tctctttttc agcttgtgct tttttctctt
    39181 cttcagttaa tttattgtta gcttgttgtt gagttgatga ctttggaata ttctctttat
    39241 catttttctt attgttgcct actgttataa aatatcctac accaacaata accactataa
    39301 ctactgctat aatagtttct tttaatctac tcttcttaga aacaacctct tcatcatcta
    39361 aagaatcttc tttatctgat acataatttt tcatttcttt cttgttttct ctatatttgt
    39421 ccttagaaga atttttagat tttttattac tattttcttt ctttttaata ggtattacta
    39481 ctgatttttc attttctaat ttttcttctt ttatttcttt tgctttttta tcttctaagt
    39541 ttttactttc tttttcaagc ttcgattcat tatttgaaga tactttttca tctaagttat
    39601 tatttattgg tttttctatt tttacactac gttcttcttc tatatgttga cttggttctt
    39661 cttgttttat ttctttcaat tcattttgct ctatttcttc tgatttcact tgatgatttt
    39721 caactggttt atctaaaatt atatctggag aaatatcatt taaattctct tcttcttgtt
    39781 cagcttcttc attgtctttt ttaataaatc caaataattt agccttcttt ttactctttt
    39841 tattttctgg ttcaattata tcttctttat tgctgtcgta ctctttatct gtagtatctt
    39901 tatcttctac aacgtcacct atatcttgat tattactatc tacttcttct ttagtccctt
    39961 cttcagcatc atcttctatt gaaatactac ttctaaagca aatatcttta tctgcatttt
    40021 ttatcatgta aatgtattct tcaatatcga tatctttatt tcttctagtt ctttttgatg
    40081 caatttcatg attttctaat tcactatagt tatcttttat tagattctgt gctttattcc
    40141 atgtatcttc actaatttct gttttatttt taattacata gtccacaaat tccttaaaat
    40201 tttcaggacc aaattctcca attatagtca gtctcattgc accttctata aggtctctat
    40261 ttcttctctt taaaaattct aatacaactg ttgcacaagc aaaatcttta ataatttctt
    40321 tgccaaaacc atatgcttca acagaatgaa gttcttctaa tccacataac ttcttatatt
    40381 cattgtgtat tgtttgagat atactttcat tatttaagct atttaactta tctataaact
    40441 tcgattctat atcatttgat tttgattcat gttttacttt tgaattttta atttcattta
    40501 gtagcatgtc attttctgaa ttatctttac ctaattgcaa atcctcaatt tcagttgctt
    40561 ttatttctac tttgttttgt tcgttattct cttccacatt attaggtacc acatctttat
    40621 tactagctaa tattttgttt acacagaata taatttctct ttttaattct cttttcgcat
    40681 ctgcttgatt tttaagaaat aaatctccac attctttctt ttctaaactt atttttctaa
    40741 gagatatatt aagaaggtct ttatataggt ttaaaacctt ataatccttt tctaaacgtc
    40801 ttcttctctc tgatagttcc attttatcat aagctaaaaa actatttaaa tcattggctt
    40861 ttagagagtc taatccctct ttcatagcct tatttacata ctttctacat tttgctgtag
    40921 ttaactctaa atctttttct aattcatcta tttcaggtat gtacttatct actatccatt
    40981 tgccaataag tggttctgta tttcttgcta atgtcttata aaacaatata ttaaatttta
    41041 aaagctctgc atattttgct tcattaaatt ctactttttc aaatttcttt tccaaaacac
    41101 catctagctt ttcatttatt ttagactctt gttttaaaaa acttgctgtg acatttaaaa
    41161 cagtgtttct tattatatta ttttcactca aaaccttcac tccttaaatc aattatttta
    41221 ttgtaatatt tacattgtgc aaagttgcta atttgattat gatatctaaa taattattta
    41281 ctaaatttgc tacatcttat atcatatcat attatttaca tcattttact ttttcttttt
    41341 atatctatct tatattatac catttttaat atctgtacta acttatatta caatattcct
    41401 caaaatttta aaagtagatt agacaaattt acaaataaaa aaacatcagt ttaaactgat
    41461 gtttttgact atcgaaataa ttaagcaagt ctataagcgg agttctgtat tagataatca
    41521 tctttctagg cctattgtca ccaataggct caagcgacct acctaccgga gagtacgagc
    41581 agcactcttt attcctatct tggtcttgct ccaggtgggg tttacatagc caactagtcg
    41641 cctagctgct ggtgagctct tacctcgcct ttccatcctt accacaatag tggcggttta
    41701 tttctgttgc actatcctta ggatcgctcc cactaggcgt tacctagcac cctgctctat
    41761 ggagctccga ctttcctcat gtaagattac atgcgattat ctggcttact taatatttat
    41821 tttacttagt tatgttaaca caaattcttt tacatgtcaa agtaatatta tactaaaaat
    41881 ttaaatttat tcaaaaatag tgtaatatag atatataaac tacatttaac tcgaggtgat
    41941 ctattgaaaa aattatatag aatagtaatt aacattatac tagtattagt aatactttac
    42001 agtggattta atatatactc aaaacttacc aaatacaatc atgatactaa aatatcctca
    42061 gaacttcaaa aaaaagaata taaaaaagag gatttatcaa agattaacag tgattttaaa
    42121 ttttggcttt ctgttgagaa tacaaatata aattatccag ttgtacaatc taaagataat
    42181 tcttactatc ttgacaaaga tttttataaa aaagattcta tttctggtac actgtttatg
    42241 gattatagaa ataaatctat tgatgacaaa aatatcataa tatatggaca taatatgaaa
    42301 aataaaacta tgttcaacaa cctaaataag tttaaagatg ctgacttctt taaaaaaaat
    42361 aataaaataa aaataacctt gaatggaaaa gaatttttat atgatgtttt ttctgcttat
    42421 attgtagaat ctgattatga ttatctaaaa acaaatttta acaatgaatc tgattatcaa
    42481 aattacataa atgatattac ttctaaatca ttgtataaat ctcctattaa ggtaaatagc
    42541 aatgataaaa tagttacact ttctacatgt acctatgaat ttgatgatgc cagaatggtg
    42601 attcatggta gattgattta aaactcacag acacagttta aaacaataat tttaaactgt
    42661 gtcatctttt atacctataa tttgtaaatt agaccactaa aagtatttaa tacaaaataa
    42721 caaatacatc aaacaataaa aaacaataaa aaaagactat gctaaaaaca tagtctttac
    42781 tggagctggt gataggaatc gaacctacaa cctgctgatt acaagtcagc tgctctaccg
    42841 ttgagccaca ccagcacacc tattttattg taaattagca aaactaattt ttaaaagtta
//
LOCUS       DAEMGG010000019        42802 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEMGG010000019 DAEMGG010000000
VERSION     DAEMGG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.019"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            456..4004
                     /locus_tag="KPK24_001026"
     CDS             456..4004
                     /locus_tag="KPK24_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2298731.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            4189..6123
                     /locus_tag="KPK24_001027"
     CDS             4189..6123
                     /locus_tag="KPK24_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG2298732.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6229..7224
                     /locus_tag="KPK24_001028"
     CDS             6229..7224
                     /locus_tag="KPK24_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2298733.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7196..7981
                     /locus_tag="KPK24_001029"
     CDS             7196..7981
                     /locus_tag="KPK24_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG2298734.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7969..8667
                     /locus_tag="KPK24_001030"
     CDS             7969..8667
                     /locus_tag="KPK24_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298735.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8790..9263
                     /gene="ybaK"
                     /locus_tag="KPK24_001031"
     CDS             8790..9263
                     /gene="ybaK"
                     /locus_tag="KPK24_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG2298736.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9460..10299
                     /locus_tag="KPK24_001032"
     CDS             9460..10299
                     /locus_tag="KPK24_001032"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG2298737.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10312..10860
                     /locus_tag="KPK24_001033"
     CDS             10312..10860
                     /locus_tag="KPK24_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG2298738.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10926..12107
                     /locus_tag="KPK24_001034"
     CDS             10926..12107
                     /locus_tag="KPK24_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG2298739.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12402..12788
                     /locus_tag="KPK24_001035"
     CDS             12402..12788
                     /locus_tag="KPK24_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2298740.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12938..13201
                     /locus_tag="KPK24_001036"
     CDS             12938..13201
                     /locus_tag="KPK24_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2298741.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13330..14172)
                     /locus_tag="KPK24_001037"
     CDS             complement(13330..14172)
                     /locus_tag="KPK24_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2298742.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14467..15201
                     /locus_tag="KPK24_001038"
     CDS             14467..15201
                     /locus_tag="KPK24_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2298743.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            15211..16344
                     /gene="nagA"
                     /locus_tag="KPK24_001039"
     CDS             15211..16344
                     /gene="nagA"
                     /locus_tag="KPK24_001039"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG2298744.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16443..17192
                     /gene="nagB"
                     /locus_tag="KPK24_001040"
     CDS             16443..17192
                     /gene="nagB"
                     /locus_tag="KPK24_001040"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG2298745.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17367..17639
                     /locus_tag="KPK24_001041"
     CDS             17367..17639
                     /locus_tag="KPK24_001041"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298746.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            18299..19003
                     /locus_tag="KPK24_001042"
     CDS             18299..19003
                     /locus_tag="KPK24_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2298747.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            19005..20492
                     /locus_tag="KPK24_001043"
     CDS             19005..20492
                     /locus_tag="KPK24_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2298748.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20697..22310
                     /locus_tag="KPK24_001044"
     CDS             20697..22310
                     /locus_tag="KPK24_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2298749.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22673..23503)
                     /locus_tag="KPK24_001045"
     CDS             complement(22673..23503)
                     /locus_tag="KPK24_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2298750.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23576..25303
                     /locus_tag="KPK24_001046"
     CDS             23576..25303
                     /locus_tag="KPK24_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298751.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25340..27202
                     /locus_tag="KPK24_001047"
     CDS             25340..27202
                     /locus_tag="KPK24_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298752.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27535..28224
                     /locus_tag="KPK24_001048"
     CDS             27535..28224
                     /locus_tag="KPK24_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG2298753.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28320..29006
                     /locus_tag="KPK24_001049"
     CDS             28320..29006
                     /locus_tag="KPK24_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG2298754.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            29023..30930
                     /locus_tag="KPK24_001050"
     CDS             29023..30930
                     /locus_tag="KPK24_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG2298755.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKHSGNQSDEKEVNAELTSSVVKA
                     NTDNENKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            31382..32071
                     /locus_tag="KPK24_001051"
     CDS             31382..32071
                     /locus_tag="KPK24_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298756.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            32507..33046
                     /locus_tag="KPK24_001052"
     CDS             32507..33046
                     /locus_tag="KPK24_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298757.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            33065..34108
                     /locus_tag="KPK24_001053"
     CDS             33065..34108
                     /locus_tag="KPK24_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2298758.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            34110..34946
                     /locus_tag="KPK24_001054"
     CDS             34110..34946
                     /locus_tag="KPK24_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298759.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            35051..35725
                     /locus_tag="KPK24_001055"
     CDS             35051..35725
                     /locus_tag="KPK24_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2298760.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            35725..36777
                     /locus_tag="KPK24_001056"
     CDS             35725..36777
                     /locus_tag="KPK24_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2298761.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            37007..37855
                     /locus_tag="KPK24_001057"
     CDS             37007..37855
                     /locus_tag="KPK24_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2298762.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            37845..39509
                     /locus_tag="KPK24_001058"
     CDS             37845..39509
                     /locus_tag="KPK24_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2298763.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLNKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            39558..40814
                     /locus_tag="KPK24_001059"
     CDS             39558..40814
                     /locus_tag="KPK24_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2298764.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            40837..>42802
                     /locus_tag="KPK24_001060"
     CDS             40837..>42802
                     /locus_tag="KPK24_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG2298765.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSMKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDS"
BASE COUNT        17009 a         4344 c         7406 g        14043 t
ORIGIN      
        1 ttactagaat tattatctgg taacttaagt tttgagtatt aatctaaagt atatgttttt
       61 atatggttta aattttatgt atacaaaatt aatttgctaa ctgaatcata taaaaaaaaa
      121 caaaatattt tttagaggga atgattagat agaatttttt tgttatgaaa ttaattgaag
      181 agtaattaat tagaaaagga atgaataata atgaatatat tacattatta ctttttgata
      241 aatcaaaaat aaagttataa tttttaatat taattaagaa tgatgaaata tgaataaaga
      301 ttttacaaat aaatcttaag ctatcttatt taattctaat acaggaagaa ggtaaatgta
      361 tattgaaaaa atcatagaaa tagaagtaaa caatttaaat aaatacaaag gtttgcaaaa
      421 gaagtaatat aattatatac aatgaggatg tgagtatgat gaacagacta aagccaagaa
      481 ttttaggtat tagtatcttg tttattatta taattatttt aacatgtgga gctgttttca
      541 agtttaaatt taaattagat aataaattaa aagaaaccac acatacatat ttgtctgagt
      601 ttacagaaca caatataatt aatctcaaga caagacttag tggtcaattt gacatgcttg
      661 aatctattgc taacttcata ggaaacttga ataatataaa tgatgaaatg gtgattgatt
      721 taatggattc tgatgtaaag cgaagttctt taattagaat gactatctct acattagatg
      781 gggaatgtta ttctaatgat ggaattaccc ataatgttca agatagagat tattttaaaa
      841 aagctaaaaa tggagaaaag aatatatcag agcctttaaa atctattgtt aataataaag
      901 aaataatagt tttatctacc ccagtttata aaagtgggaa aatagttggt gttttaagtg
      961 gaatttatga ttcaaaaaag ttaaatgagt tattaggatt atctgcattt gatgaaaaag
     1021 cagatgtttt tgttgcaaag aaaaatggag atatagttgc tcaaaaaaca aataaaaata
     1081 gaaagacgct atcatgtata tttcagcttt ttgacaaaga taattgtgat gaagatgatt
     1141 gtctaaaaac tataaaagaa aatatgaaaa atgataagtc tggatatttt aattataact
     1201 tagataatga aaaatttatg ttaaactatc agccacttgg agttaatgat tggtatgttt
     1261 tttctgttgt gccaaataaa atagtatcta ttcagtcaga gaatatatct tatgatgtat
     1321 atatttttgc attagaaata ctactagtta tattgatttt tataatatat atagcatgta
     1381 ttataaataa taacaccaaa attattataa atgaaaaaga aaaatttaaa gtactcactg
     1441 ataatatatt aggaggggtt aaaacctgtt taaaagatga atatatgact atgacatatg
     1501 taagtcaggg gtttttagag cttacaggtt actcttgtga agatttaaat aaaatattta
     1561 aaaatagatt taaagacatg atttataaag atgatttaga caagtttaat tgtattattc
     1621 taaatcaatt agaaaaacaa gataaaattg aagttgaata tagaattgta agaaaagatg
     1681 gaagtataat ttggattttg gagagaagta agtctataaa tgataataaa ggaagtgagt
     1741 gtattcaggg agttttaact gatataacag aacttaagaa aatacaagaa gaattagatt
     1801 caaaaagatt tgaaatacag gctatcaatg atagtattgt tggtggaata gtgataactg
     1861 agataaatga tgattttgat agtatatatt taaatgaagg ttatttaaat atgattggat
     1921 atacacgcaa acaactggaa gatgagctaa ataataaatt aatcaaactt atttttaaag
     1981 atgatagaac agaattaatt aatagtataa acaaacaact caaattaagt gatacatttg
     2041 catctgaaca aagagttgaa aaaagggatg gcactattat atggatttta ttaaaaggaa
     2101 gaaagatgat ttatgataat ggaaaatctg tactttgttg tgttattgta gatattactt
     2161 ctgctaaatt attagagcaa gaacttaaaa agagtgaaga acgtcatgaa atcattctaa
     2221 agcagacgca aaatattata atagaatggg atatagaaaa agataaaatg aatatatcac
     2281 ctaattggta taataagttt aaatatgacc ctataactga aaacgcaaca aaaaatataa
     2341 ttaaatcagt agatatatat catgatgata tggataagtt tatttcatta attaaactta
     2401 cttcacaagg tagtgagtat gaagagtgtg aaattagact tagaactaga gatactattt
     2461 acgcttggtt tagtataaag gcaacaactt tatttgataa taatggtaaa ccttataaag
     2521 tagtaggaag tttgactgat attgatgaat caaaacgaga agctgaaaag ttgaaagaaa
     2581 gggcagaaaa agaccctctt acagatctgt ataataaaaa agcatcacaa tcacttatag
     2641 aggagtacat aacattggat aaaaaaagaa aaggtgcttt aatgattatt gatattgatg
     2701 actttaaagg aataaatgat aatttaggac atttatatgg ggatgctgta ttaagtgaaa
     2761 ttgcaaatga cctaataaat ctatttagag caaccgatat agtaggcaga attggtggag
     2821 atgagtttat tgtatttata aaagatattt ctgaaacaag agatattata aataaagcag
     2881 aagaattggt aaaaacattt gaaaggtctt ttttaggtaa aggacgaaat tataaaatat
     2941 cattaagtgt aggaattgct aagtttccac aagatggtat ggactatatg acactattta
     3001 gaaatgcgga tatagctctt tactcagcca aagggaaggg caagaattgt tatacattgt
     3061 atgataaaaa atttgataaa gtccaatata taactaatac aagaaatgca ggagatagtg
     3121 ataatgtaga gatgtctttt aaagaacata tcacaaatta tatatttgat atactatatg
     3181 aatcaagtga tatagaagtg gcagtaaatt taattctaag tattgtaggt aaacacttta
     3241 atgtgagtcg catgtatata tttgaaaatt cagatgataa tcgagtatgt aaaaatacat
     3301 ttgaatggtg taacgaaggt gtaacctcgg aaattaataa tcttaaaaat atttgttaca
     3361 aggatttagg caactatctt ttaaatttta atgcagatgg gttattttat tgtaatgata
     3421 ttgaaaattt aggtagtgat gtgaaaaaag tacttaagcc acaaggcata cgttcaatat
     3481 tacaatgtaa aattttagag ggtaatgaat ttagagggtt cataggattt gatgaatgtt
     3541 catataatcg tatgtggact aatgatgaag tgaacacttt gacatttata tctaaaacgc
     3601 tcagtatgtt tttattaaaa atgcgtattc agagtaagct agaagagtct ttaacaattc
     3661 aaaaatctgt gatggataat atggatatat gggcatatgt tgttgacagg aatacctatg
     3721 aattactatt tatcaataaa aaaatgcttg agtcagcacc aaacactcat gtaggtgatt
     3781 tatgttataa agcgatatgg aataatcaag ataaaccttg taaaaaatgc cctatgatat
     3841 atttaaaaga tggtgatgat acttataaat cattcatgca taataaaaag tttgaggtac
     3901 aggcaaatat aactacaact actttgaaat ggtttgatgg taaagatgca tgtttaatat
     3961 ttggaagtga aataactagt attgaagctg aagaaaatac ttaaaatact gtgttatata
     4021 tatacttaaa atcgcaattt gaatttatta gacttaaaat gtctatataa atcatttaag
     4081 ttgtggtttt aagtattttt tatgatataa ttaagcgtta agaacaaaat taaaagatac
     4141 ttttgtataa aaaaatattt gttaaatata tattgagcag gtgataatat ggaaaataag
     4201 tttttaccaa taagcaaaca agatatgata gatagaggat gggaagaatt agattttgtt
     4261 ctagttacag gagatgctta tgtggaccat catagttttg gaacagcaat aatatctaga
     4321 gttttagaaa gtgctgggta taaagttgga ataatagctc aaccagactg gagaactaca
     4381 gatgatttta tgaagctagg aaaacctaga cttgcatttc tagttaatgc aggaaatatg
     4441 gattcaatgg tcaatcatta ttctgtaagt aaaaagcata gagaaaagga tatgtattct
     4501 ccaggtggga aaatgggtta tagaccagat agagctacta tagtatattg caataaaata
     4561 agagaagcat acagtgatgt tgcaatagta ataggtggaa ttgaagcgag cttaagaaga
     4621 tttgcacact atgattattg gagtgataag gttagaaagt ctatgttaat agatagtgga
     4681 gcagatttat tagtttatgg tatgagtgaa aagcagattg tagaagtagc caatgcactt
     4741 aatgatggat atgacccaaa atacattaga catattgatg ggacatgcta tatttcagat
     4801 acattggaag aaatatatga taaatatata ttaattccat catataaaga aatttgtgaa
     4861 gataaaatga agtatgtaga agcttttaaa attcaatatg atgagcaaga cccatttaga
     4921 ggtaatataa ttgttcaacc tcatggaagt aagtatctgg ttcaaaataa gccagaaaaa
     4981 ccacttagta gagaagaact agatgaagta tatggattac catatcagaa gacataccat
     5041 cctgtgtatg aaaaatttgg aggaatacca gctatagaag aagtaaaatt tagtatagta
     5101 agttcaagag gctgttttgg tagttgttca ttttgtgcaa taacatttca tcaaggtaga
     5161 gcagtgcaaa gtagaagtga aaaatcaatt attgatgaag caataggaat aacaaattta
     5221 gatgatttta aagggtatat acatgatgtt ggaggtccaa cagctaattt tagaagacca
     5281 gcatgtaaaa aacaaattac taaaggtgct tgtaaaaata gacaatgctt atctccatca
     5341 ccatgtaaaa atttagatgc agaccacagt gaatacttac atttacttag agctgtaaga
     5401 aaattaccta aaataaaaaa ggtgtttgtg cgttctggaa ttagatacga ttatgtaatg
     5461 gcagataaga acaataaatt tcttagagag ttgatagagc atcatgtaag tggtcagtta
     5521 aaggtagccc cagaacatat atcagaagaa gttttaaagt atatgcaaaa accagcagga
     5581 aaaacttatg ataaatttag acagaagttt tttgctataa atgaagaatt aggtaaaaag
     5641 caatatttga taccctattt aatgtctagt caccctggtt ctaccttaaa tagcgctata
     5701 gagttagcag agtatttaag agatactcac taccaaccag agcaggttca ggatttttat
     5761 ccaactccag ggactctatc tacaaccatg ttttatacag gtattgaccc gcttacaatg
     5821 aagccagttt atgttcctaa atcaaaacgt gacaaggcga tgcaaagggc tttattacaa
     5881 tatagagcac ctagaaatta tgatttggta tactctgcgt tagttgaagc aggaagagaa
     5941 gacttaattg gttttggaca tagatgtctt ataaagccaa aaaatgaaaa gccttatttt
     6001 aatagaaata attctaaaaa aaatgtatct aaaggaacaa ataaaaacaa aaaaactaat
     6061 acaaataaca gaagtaataa aaatcaacaa aaaagttcaa ctaaaaaaaa gaaaaaacat
     6121 taaagtataa ataaaaatac ttaaaaaaga aggattattc gataaattaa cagaaattag
     6181 tataaataaa tataattatt ttaatgacgt tacctttggg aggaagagat gaaaaagtat
     6241 atttctataa tattattagt ggtgttgact atggttgttt tggtaggatg ttcaccagga
     6301 aaagataacc ctaaaaataa ggagctatca gttgtcaaac aagtaaaagt agctgttcca
     6361 gatggactac cagcagtggc aatagctaaa cttgcaaatg aaaatccaga gattaaagaa
     6421 ggatatgaaa cagtgtatag cattgaaaag acacctgaag cgatttccac tagagtgatg
     6481 aaaaaagatg cagatatagc tatagttcct tcaaacatgg ctgcaatagc gtataataaa
     6541 acttcatcat ataatattgt tggaactgta ggaatgggtt cactttattt ggtgtcaaca
     6601 gaaaatataa aagattattc tgatttagtt ggtaaagaag ttggatgtac tggaaaagga
     6661 cttactccag acataactat aaaaagtctt ttgttacaaa aggatataaa ttattcagat
     6721 ataaaattta attatgtaaa ttcagcaagt gaactggtac cattattggc tactggtaaa
     6781 gttaaaacag gtattgttcc agaacctgca cttagtgctt tgatgtctaa aaatccagac
     6841 ataaaaatta taaaaagttt aaatgactca tggaaggaag taagtggttc aaaagatggt
     6901 tatcctcaat ctactttaat tgtaaagtct gaatttttaa gagataataa agattttgtt
     6961 gattcttttg taggtcaatt atcaaatagt atagattggg caaacaaaaa tcctgaagaa
     7021 ttaggtgcat attctgaaaa aataaaaata tctactgaat caaagataat aggaaaatct
     7081 cttgaaagag ctaatttaaa atatattcca gttaaggaca tgattaaaga ttataaaaat
     7141 tactatgaga aattagctaa ttttgatgat aagacattag gaggcaaagt accagatgaa
     7201 gcaatatact ttgttgagaa ataaaaaaaa taaagaacaa tttatagata aaatggaggt
     7261 aacactttct tgtgttatcc tcttattttt atggcaaatt attgctttaa aaattgataa
     7321 tgatattttt ttaccaacag taggtcaagt atttaatagt ataaaagaga ttatgctaaa
     7381 ttctagtttc tatatagata tactttactc tatggggaga tgttttttta gttttttact
     7441 ggctatgatt tttgctatta tactttcaat agtgtcttat ttaaatagat tttttagaaa
     7501 cttattaaag ccaatcaatg ctttgacacg ttcaatacca actatgatat tggtagtact
     7561 tgcacttatt tggtttgaaa aggatagtac cccttttata gttggattta ctatagtttt
     7621 tcctatattg tatgataacg ttttgggcgc tatattgaat atagacaaga acttattaga
     7681 aatggctaat gtgtataaaa taaggtttat agataaggtg ttaaaaattt atttaccagc
     7741 cataaaattt caaattatat ctatactagt atcaacattc tctttaagtt tgaaagttgc
     7801 tatagcagga gaggtatata gtcaacctac atatggtatt ggaactatga tacagactga
     7861 aaaaattaac tttaacacat ctggaatatt tgcatggata attattgttc ttttaatatc
     7921 tgctatttta caaatagctc aaaagttttt agcaaggaga gcttttttat ggaaaagata
     7981 aagttgagta ttgaaaatat aaataaaaga tatgatagtc gtattatatt tagagatttt
     8041 aatattgatt tttatgtaaa tgaggtaaat tgtatccttg gaaaatctgg ttgtggaaaa
     8101 actacactct tgaacataat aagtgggatt attaaaaatg acaccaacaa tctaaatatc
     8161 aaagaaaatt taaatagagt aggaaataag cttgaagcta gttacatatt tcaagatgat
     8221 agacttatag actggttaac agtagaagaa aatataaaga tagttgtaaa taaatactat
     8281 aataaaactc aattaaataa aatttgtgat gaatacttgg aattggttgg aattagtgat
     8341 tataaaaaat tctatcctca gatgcttagt ggaggtatta gacaaagagt aaatattgct
     8401 agagctttta tatatccatc aaaaaatatt ataatggatg aaccttttaa atctattgat
     8461 gcaaagaata cacagcttat aatggataat ttcagaaata tattaagaaa agaaaaaaga
     8521 actgtgttat ttgtaactca taatattgag gaagcattgt ttttagcaga taggatattt
     8581 attttaggag actctccaat tagaataaaa aaaatattaa aaaattctaa ggaattggaa
     8641 aaaaatgaag ttttgaagtt gatatagagt tataataata ctgtcgtttt aaaatatatt
     8701 tggttaaaat ctataagact atattaaaat agtacagaat attcaaaact gtgtataatg
     8761 ttcgtatttt ataaaaagga agtgaatgta tggttaagac taatgctatg agaatattag
     8821 attcaaataa gattgattat aaagtaatgt cttatgaagt gaaaagtgaa catgtggatg
     8881 gtgttgaggt ggcacatgat ataggaagag atgtaaatga agtgtataag acattagtaa
     8941 ctcaaggagt tagtaagaat atatatgtat atgtaatacc agtacatgaa aacttagact
     9001 taaaaaaggc tgcaaaagta gctaaagaga aaagtgtaga gatgattcat gtaaaagata
     9061 taaataagct tacaggttat attagaggcg gatgttctcc tataggtatg aaaaagcttt
     9121 ataaaacttt tgtaaatgaa agtgcaaaga atttagatac aattattgtg agtgcaggaa
     9181 aaataggata tcaaatagaa ctatctccat ttgatttaca aagacttata aaagtagaat
     9241 ttgtagatgt aataaaaaaa taagaattac atataaaata tagagcttat attcttaata
     9301 ataggtgttg tagtattaat aaaatgaata ataattttaa aataaatatt cagttactta
     9361 tgtgtaattg gtataaaaat attgagaaat tttaaaaaca atggtaaagt gtaagtataa
     9421 tcacgaatat tttttataaa gtaatggagg gatgttcgct tgagaaaaat aaaatctgaa
     9481 caaatcgtcg agcaggtaaa aaaactttgt atagaagcga gcctatattt aggagaagat
     9541 gttttaagct gtataaaaga aaaagcgaaa agtgaaaaga gtgaagttgg aaaaaatata
     9601 ttgaatatat tagttgaaaa tgctgaaata gctaaagaaa aaaatatacc aatatgccaa
     9661 gatactggta tggctgtatt ttttgttgaa ataggacaag aagttttaat tgaaggagac
     9721 acacttactg atgctataaa tgagggagta agacaagggt atgaagaagg ctacttaaga
     9781 aaatctgttg taagtccaat taacagagta aatacaaaag ataatacacc tgctgttatt
     9841 cattatgata tggtaaaagg tgataaaatt aaaatagaat ttgcagctaa gggatttgga
     9901 agcgaaaata tgagtaagat gaaaatgtta aagccatcag atggattgga aggaattaaa
     9961 aaattcataa tagatacagt ttcagaagct ggacctaacc catgccctcc tatggtaatt
    10021 ggagttggta taggtgggac tgtggacaaa tgtgctcaaa tagctaaaaa ggcacttttt
    10081 agagaactag gtgaatttaa taaggacgaa aatatagcaa aacttgaaag tgaactatta
    10141 acagctatta ataaacttgg tataggacct caaggcttag gtggtactac aacagctttg
    10201 ggattaaaca tagagacttt tcctacacac atagcaggtt tacctgttgt tgtaaatata
    10261 aactgccatg catcaagaca taagaaggta gtaatttagg aggaaaataa aatgataaaa
    10321 ataactacac ctgtaaatga aattgatata gcaaaattaa attgtggaga tacaattagt
    10381 ttgagtggta tactttatac agcaagagat gctgctcata aaagattgat agattgtata
    10441 aataaaggtg aagaattacc atttgacgta tatggtcaag gaatatatta tgtaggacct
    10501 actcctacaa agccaggaga agttataggt gctgcaggtc caacgacgag ttatagaatg
    10561 gatgatttga ctatacctct tttagaaaga ggtcttagat tgatgatagg caagggaaaa
    10621 agaagtgatg aagttataga aggtatgcaa aaatacggag cagtttatct tgctgcaata
    10681 ggaggagctg gagcatatat ttcaaattca ataaaatcat gtgaaattat tgcatatgaa
    10741 gatttaggag ctgaagctat aagaaagatt gtagtagaag accttaagct tacagtagca
    10801 atagatagct atggaaataa tatatatgag caaggtcgag caatttatga atgtaaataa
    10861 tgtttagaaa taaatatgaa tatcaaaatt aaaaatagtt gtgtatattt taaggaggaa
    10921 aattgatgtc taaggattat gcaaaattgg cattagaaat gcatgaaatc aacaaaggga
    10981 aagtaagtgt agaaagcaag gtagaagtta aaactaagga tgatttatca acagcatata
    11041 caccaggggt tgctgaacct tgtttaaaaa tacatgaaaa tcaagatgat gtttatagat
    11101 acacttctaa agggaatcta gtagcagtag ttagtgatgg aagtgctgta cttggattag
    11161 gtaacattgg tgctgaagca tctatacctg ttatggaagg taaagctata ttatttaaac
    11221 aatttgcaga tgtagacgct tttccaatat gtttaaagac taatgatgta gatgagatag
    11281 ttaaatctgt agagttgatg gagcctgtat ttggaggtat aaatttggaa gacatatctt
    11341 ctccaagatg ttttgagata gaagagagat taaaaaaatc tttaagtata cctgtttttc
    11401 atgatgacca acatgggaca gcaataatag ttgcggcagc cataataaac tcaataaagt
    11461 taattgaaaa taaaaaaata gaagatttag aaatagttat aaacggagca ggagctgctg
    11521 gtatagccat agcaaagata ctacttaata tgaatgttaa aaatataatc ttatgtgata
    11581 gaagtggagc attggaagct agtatagaaa atctaaacta tgttcaaaaa gaaatgttaa
    11641 aagtaacaaa tataagaaat gaaaaaggac ctttaaaaga tattataaaa ggaaaagatg
    11701 tatttattgg tgtttcaggt ccaggagctg ttacaaagga aatggttgaa agtatgtctg
    11761 agaagccaat aatattggca atggcaaatc ctactccaga gataatgcca gaagaagcaa
    11821 aattaggtgg agcatttata atgggaacag gtcgttcaga cttccctaat caagtcaata
    11881 atgtactggc ttttcctgga atatttagag gtgcacttga tgttaaggct aaagagataa
    11941 atgaagaaat gaagatagct gctgcatatg caattgctaa tacaataagt gatgaagaaa
    12001 tatgcccaga gtatatatta ccagatgtat ttaataagaa tgtagtaaaa aatgttgcta
    12061 aggcagttaa agaagcagcg ataaaaacca aagtaaatag aatataaatt tttatatgtt
    12121 catatatcct tatattatcg ggtatatggg catttttatt ataggtttat actcatatat
    12181 ccttactttt atatacatat ctttatgatt aaaggtgtta gaaaatgtag taaattttat
    12241 actatcagac tctatcttta ttactttagt agatacatgg ttgtaaaggt ttatagaaaa
    12301 aaatatagga aggcagattt ttattgttgc gtatatatga gaatgatata atatgaatat
    12361 tataaattaa taaatttaaa tttatggagg tacaagagat gatggaatat aaagttaatg
    12421 accaagaact taatgcttca atgtttatcc cattcgtcaa caggatatgg ccaggagatt
    12481 atgatatgga taaaacacag actgcattat caaaaacttt gaatataact gcttatgatg
    12541 ataaagtgct tgtaggatgt ctgcgagttc tttcagatgg ttattatttt ggaacaatta
    12601 cagaattgct tgttcttcca gagtatcaga aacaaggcat tggtagtaag cttttccagc
    12661 ttgctaaaga gaacacccca actatgctat attttggtgc acagccagga gtagaagagt
    12721 tctatgaaaa aaatgggtgc caaagaagtc tgcaatccta cataataaaa aagaaagata
    12781 gttattaaag ttccagcttt caataatctt aaaagtacaa tattttagtg gaagcatttc
    12841 attttttatt actaatatga cataaacatg tcatattagc attgatatac ttttaatatg
    12901 gttaagagcc aaagaaaaat ataaggagtg atttaaaatg gaagaaacta aattttgcca
    12961 atgctgtgca atgccaatgg gacaaactga tgaattatat gggacaaata aagatggaag
    13021 taaaagtact gattattgta gttattgttt taaagatggt gagtttacag ctgatataag
    13081 tatggaagag atgatagaag tttgtatccc tcatatgctt caaagtaata aggatatgac
    13141 tgaagatgaa gcaagaacaa tgatgaacaa tttctttcca acattaaaac gctggaaata
    13201 attttatgca aaattagtta tatgcaaaac atgaaatatg gtgttgagaa taaggggtgg
    13261 tgtgctttat agcataccac ctgttttgtt acttggagtt cagttatttt cctaaatatt
    13321 ttaaatatac tattttagat aagttccctc ttcatcttga taaattaatc cttttttcat
    13381 aagcactcca agagctcgtt taaaattttt cttacttgaa ttaaataata tagaaatatc
    13441 tttaggatca gatttatcat taaatctcat atatccatca gaaccttcta aataacttaa
    13501 aatcttattt tctaaagtat caagttcatc ctttctattt cctctaggag aaagaccaag
    13561 ttttccatct tcatatattt ttatgacatg taaattttca agaacatcac cagtttttag
    13621 ttcagtaaag tactcattat gaagtattac accagcatat ttattgtcta cacatatcat
    13681 tgcagaatta ttagtttgga atccatatac aactccagtt acaatatctc ctacattgta
    13741 agtatggtct gttaataaat atgaatctat atctgtagta gcagcaattc ttttgctttt
    13801 gtctaaatat atataaaata aatatttttc gtctctaaat agtggataag tcttagcttt
    13861 aaaaggaact aatatgtctt taggaagtcc catatctata aaagtaccaa tgtctgtatt
    13921 atcaactact tttaaatgtg ctacatcccc aaccttagct aaaggcggta ttaaagttgc
    13981 agcagttctg tcatcagaat ctttaaatat gaaagcattt acttcatcac ctatgtttat
    14041 ttcatttttt ccgattaatc tattatgaag aagtatatcg tcttttgtgt tattagtttg
    14101 tccatccaaa aaatatccaa attcagtttt tctttttact gtaagtttat taaaatcacc
    14161 tatgtttatc aaattaaaat catcctctca ttttatattt ataatttagc cataatatac
    14221 ttatgtagta gtttgtcaca gtatctatat tagtattagt gaaatttaca ataaataata
    14281 tagttattta tgtatttttc aattctctag cttcttatat agtataaact ccaatttctt
    14341 ttttgtcttt aggttttatt aaaattttta caaaataagt taaaaagtat tgaaataaaa
    14401 tttcttttaa agtataattg gtatatacca aaattggtat ataccatatc gaaaagtggt
    14461 gactatatga aaatagtaaa taaatctagt aatataccat tgcatactca attaagttca
    14521 ataataagag agatgataga aactggtgag cttaaagagg gtgatgcaat aatgccagaa
    14581 agagaacttt gcaacataca aaatgtaagt cgtatgacag taaataaaac cattgttgga
    14641 ttagttacag aaggacttct ttatagggta caaggtaaag ggacattcgt tgcaaagcaa
    14701 aaaaagaagt atcagttttc aaatgtaaaa ggttttactg atgttatgaa agaaaaggga
    14761 gtaaacataa aaacggatat attatccttt gagatggaac taccagatga tttagttaaa
    14821 agaaagcttg gaataagtga taatactaca aatatatata agattgtcag acttagatat
    14881 actgatggag agccatttgg attagaaata gtatatctat cagaagaaat gtgtaagggt
    14941 ctaacaaaag ggatattgga taattcttca ttatataggg ttttaaatga aaaatatggc
    15001 tataagatac aaaaagctga acaggtaatg gagccagtaa tactttcaga tgaagaaagt
    15061 aagcttcttg aaacagatga aggtgcttta gctcttaaac ttcatagaaa ttcctataat
    15121 agagaaggta gccctataga atataccata tcaatattta gaacagataa atatcaatat
    15181 gaaatagtat tgagtgagta ggtgaataat atgaaatgtt taattaatgg aaagataata
    15241 ttaaaaaatc aaatcttaga aaataaagta ttagtatttg atgaaaaaat aattgatata
    15301 gctgatagtg taccaaaaga ttgtgaagtt atagatgctg atggaaaata tatatctcca
    15361 gggcttattg atattcatat acatggaaac atgggaaaag atacaatgga ttcgactgat
    15421 gaatctattg aaacaatttc aaagtcaatt atgagacatg gagtaacatc ttttcttcca
    15481 actacaatga ctatggataa agaacatgtt tatgatgcac ttgaagttat taaaaaagcc
    15541 caaaatagaa agcttgaagg ggcacaagta ttaggagcac atttagaagg accttttata
    15601 aatgagaatt ataagggagc acaaaatgaa aaatttataa taaattcaaa atacgaattt
    15661 ataaaagaat ataaagatgt tataaaagtt ataacttatg ctccagaaaa agatattgat
    15721 tttgatttta ctagagaaat aaaaagatgt acagatatag ttttatctat tggtcatagt
    15781 aatgcaaatt atgaccaagc taaagaagct ataaatttag gagtaactaa tgtaactcac
    15841 atgttcaatg caatgactgg tttaaaccat agagacccag gcgttgttgg tgcagcactt
    15901 acaactaatg tgtacagtga gttaatagca gatacaatcc atataaataa agatttattt
    15961 caatttatac taaataataa aggaaaagaa agactgattt taataacaga ttctatagaa
    16021 gctggtggtc ttgaagatgg caattattct cttggaggtc agaaagtcat agttaagggt
    16081 aatgaagcta gacttgaaaa tggagcatta gctggaagtg tgttgtcttt aaataaaatg
    16141 gtatttaact tcttagataa tacaaacctt aaagtgaatg aagcaattaa tttagcttct
    16201 ttaaatccag caacatcttt gggaataaat gacaaaaaag gaagcttaga aataggtaag
    16261 gatgcagata tagctgtatt tgatgagaat ttagattgta aaatgacact ttgcttgggg
    16321 gacgtagtat ataaaaatat atagtataca aaacaaacct taaagacagt aaaaacaata
    16381 taaaaaataa taaattaaac ttacattagt aaaagtagta gttaaaattt aggaggaata
    16441 agatgagagt attagtgtgt aaagattatg atggaatgag taaaaaagcc gctgaaatga
    16501 tagcagctca aatagtttta aaaccaaatt caatactggg actagctaca ggaagtacac
    16561 cagtaggtat gtatagagat ttagtaaaaa aatacaatga taatatagta gatttttcag
    16621 atgtgatgag ctttaattta gatgaatatt ataagttacc aatatctaat gaccaaagct
    16681 atgattattt tatgaaagag aatctattta atcatgtaaa cataaagcca gaaaatactc
    16741 atctaccaaa tggaatggct gatgatattg aaaaagagtg tatgaactat gaagcttcta
    16801 tagatgcagc tggtggtata gatgtacaag tacttggaat aggtcgtaat gctcatatag
    16861 gttttaatga acctgatact aaatttgcaa agagaactca tgtagttgaa cttacagagt
    16921 ctacaataga agctaatgca agattcttta aatcaagaga agatgttcct aaaaaagctg
    16981 taagtatggg aataggttca atattgaagt ctaagaaaat attgctttta gcttcaggag
    17041 aagaaaaagc tgatgcagta tataatactg tttatggaga tataactcca gaagttcctg
    17101 gttctatact tcaacttcat aaagacacta tagttatagt agatgaagca gcagcaagta
    17161 aattgaatcc aaaagattat aaattagtat aagaatagaa tgtagtttaa gtaaaaaata
    17221 gctatcattg agtaatcaaa tttatttaat gatagctatt tttatttaaa ttttttactt
    17281 ctttttattt aaattagtta ttattgttta aattacttgc ttattacttt ttacgaaaac
    17341 tcgttatcaa gaagtatcca aataagatgt taaaaattat tatgtttagt gggattctat
    17401 caagcataat aaaattacct ataattggga tattaccaac tggaaacata aagaagaata
    17461 caggcatcag tgatagtaaa aaagatggaa aacaaagaag aagtaatctt ggaatatcaa
    17521 tataccaact accatccttt ttgtattctc taatcaataa ttcagttcct agtaataaac
    17581 caataccacc agaggaaatt aaattaataa aaatgtatct tttaaagtct tgcatataac
    17641 cagtgtaaaa ttgagtatag aaatcataat ttaatttaac ggctaaaaaa tacataagca
    17701 atagaatgat taaaaataaa agatatctaa gagctttttt cattttaaat tctcccttca
    17761 gaaatacttt atttagtttt attttgttgg aatctgattt tatcatttag tttaagaaat
    17821 ccataatatt agtgaagaat gaaataagta ttatatatga ataaatattt ttagaattaa
    17881 ggtgaattgt agtgcttaat ataactataa tattaattgt ttgaatattc aataaaaagt
    17941 ttgaaaaata tcaattctga tataaactga ataaatacag ttgaatatta aattattatt
    18001 ttatcaaaaa tatgcaaagt actacacctt caaatatcac ctaaagtaat taaaaggcaa
    18061 ttaaggtgaa gtagttcaag tatttctaaa ataagtttaa taaattctat aataatttat
    18121 tttttgcctt aaaattgcct taaaatccaa acaattttaa aactaatgta ctataaaaag
    18181 ctggtataat ttaaatatag taaaaaataa gatttatctt atctattggg atttaattat
    18241 ttaatcaata tatttatacc atttaattta aataaaaagt ctattaagga gtgagcttat
    18301 gagtaagtta atttatatag cagatgatga agataatata agaaatttgg taaagacatt
    18361 cttaaaaaat gaaggtcatg atgttatgga ttttaaaacg ggtgatgaac ttttagaaca
    18421 atttaatata aaggagtgtg acttagtaat actagatata atgatgcctg gctcaagtgg
    18481 ttttgaagta tgtacaaaac ttcgtgagaa aagtacagtc ccaataatca tgcttactgc
    18541 aagagataca gatatagatt atattacagg gataacctta ggtagtgatg attattttac
    18601 aaaaccattt agcccaatgt ctttggttat gagagtaaaa tctatattta gaaggataga
    18661 gtttgaaaag aaacaaaatt atgataaata tagtaactcg atagatatgg aattaaaatt
    18721 tggtgatgta ataataaata aaaagaataa aatcgttaca agtaaaaatg taaatataga
    18781 ccttacacca aatgaatata acctattgac ttatttgttt gaaaatatag atagagcagt
    18841 ttccagagat gaattattaa acaaaatatg gggatacgat atagaagttg agacaagggc
    18901 tgctgatgat acagtgaaga gattacgtaa aaaaatttta aatacaaata ttttaataga
    18961 aactgtatgg gggtttggat ttaggttgaa ggagaaaagt taagatgaag aatggaaatg
    19021 acaagaatag aattggaaag attaaatcca aatttaaatt aaagttctca aagaaaaata
    19081 taagcataaa aactaggatt ttgttgagta tactttgttt gataattata gtatttatga
    19141 taatattagt atcttttaat gtatttcttg atacatatat aaaaacaact gcgaatgaag
    19201 aattgacaaa gtctacagaa atagtagaac atatggatag taattttaga cctattaagc
    19261 caccacaaga tgatggaaag cttcctccca aagtattatc taattttatg agaaatgtac
    19321 aagataaagt taggatggca gaaacacaat cagatgctga tgctatggta gttgattcta
    19381 agtataacct gatatttcca acaagagagg atgatttctt aaaaaatgtt gatgagatgg
    19441 agctgattag ggagtgtatt caaaatgaaa aattagattt aaattcagat gaaaatacac
    19501 ggatatctac cagaaataag gattattata tttcaactgt aaaaattact agtatatatg
    19561 gtgaacaaga caagtatctt atacttttta tagacatatc aaaaactcta aatctagcac
    19621 aaaagataaa tattgtatta atatctgtaa tgtgttttgc tggaatatta gcaatattta
    19681 cagctgtgat tttatctgag aaaattgcta aaccgataaa agagttgtgt gagtttgcaa
    19741 aaaaaatggg tcaaggagat ttcaaaagaa cctactttga cttttcagat aaagaattag
    19801 tagagctctc aactgttatg aataagagtg ctgagtatct ggataagtat gataatgaac
    19861 aaaaaatatt ttttcaaaat gcatctcatg aattaaggac accacttatg tctattaaag
    19921 gttatgcaga agctataaaa tataatgtta tagatagtaa acatgcaagt gatataattc
    19981 ttgaagaaag tgataggtta agtgatatgg ttgaagattt attatacatt tctaaaatag
    20041 ataatataac taaagatttt gaacttgtag aatgtgattt aagagaagta ctttcaaatt
    20101 gtggagctag gcaaaatgta agagctataa ataaggggat aaaatttatt tatgaatttg
    20161 atgaagaaat ggttttattt gaatgtgatg aaaaaaacat ttccaaagca tttatgaatc
    20221 ttatagaaaa tgctttgaga tacgctaaga gtgaaataaa aatagtgtgt aagtataatc
    20281 aaaaaaatat agttgtgata atagaagatg atggaattgg aatcaagaaa gaagatttac
    20341 ctcatgtttt tgaaagattc tataagggag taggaggaaa tcatggaatt ggattatcaa
    20401 tagttaaatc tattgtaaat aagcatggtg ggcgtatata cgtagaaaat ggcaaaaaag
    20461 gagctaaatt tactatatta tttaaattgt aaaaaaatta gagaaaatat agagatgttt
    20521 gaagttgttt tttatatata tctgttgtat actgttgaaa aaagaacaat attaagctga
    20581 catatattac gatagttgac atttaaagta aatgcttaaa tatagatacg aaaggaaaag
    20641 taaaatctga agtgtattta ggtttaagta agcttcttat tttataaaaa atatgtatga
    20701 aagaacagct gatttttaat attacaaatt tagtaacggt tgtatttgaa gcttttgtca
    20761 tccatatgtt tttatcagac tttttaggtc agaaagaagg gtatataaaa gttgtaagat
    20821 atgctaaact gggttttata atatgtttag gattctgtaa tttaataact ttaaacccta
    20881 aaatcacaat gcctttaatt ttcatcctta ttttttcaac atcattttta tataaaggaa
    20941 atttaaagac acgattgttt actactgttt tattaagtat attctttata ctgtcagaaa
    21001 ttgtagttac aagtattttt gttttatttg taaaagaagg ctttgaaata atgcttgaaa
    21061 ataatagtat aagagtattg gcgacaattt tatctaaaat tgtctttttg ttgacttgta
    21121 aaattatttg tctatttaaa aaggatgtac atttagatat gcccataaag tattggctcc
    21181 ctttattttt aatacctata tttagtttat ttctgtctgt ttctatcttt gatgtaagta
    21241 aattttttag tcttgaaagt ttaaaatttc taagcttaat atctagtgtt ggtattttat
    21301 atattaattt tattgtcttt tatttattta aatttattat agataaaact aaactatcta
    21361 tgaaatatga actacttgaa aaagaaataa ttcataagga agaattaaga cttagtaatg
    21421 agtgttataa aattattgtt gaacagactg attctgtcgt tttcgaatgg aatatcaaag
    21481 aaaataaatc atttgtatcg caggcttgga ctgaaaaatt tggatataac aatgcatgta
    21541 agaatatttt taaagaaatt aaagataaag acttagttca ttctgaagat aaagctatat
    21601 ttgaaggttt tcttgaaagt attaaaaaaa agaatatgca caatcaagca gtatatcgtt
    21661 taaaaaaatc taatggtgaa tatatttggt gtagaacatc aattacaagc atttataatg
    21721 atgaaaatga acttttaaga gttgtcggag taattgtaga tgtagatagt gatataaaaa
    21781 aatatgaaga gttaagaact agagcagaaa gtgactcatt gaccaatata tacaataaag
    21841 gtacatttga aaaattagtg gaagaaacta tagttatgaa tacaggtgat aaaaaagatg
    21901 cattattcat tattgatttg gatgatttta aagaaataaa tgataatttt ggtcatccat
    21961 ttggtgattt tgtactaaaa acatttgctg ataagataca aactagtttt ggtagtaaag
    22021 acttagtcgg aagaattggt ggagatgaat ttgttgtata tatgcaagat tatgttacag
    22081 aagtaaattt gcataaaaaa gctaaagaat taaatagagt actttcggat aattacactg
    22141 atttgagttt tagttttgat gcttctgtca gtataggtat tgcaaggtat cctcaagatg
    22201 gaacttcatt ttttgaattg tttaaaaatg cagatagagc actttatagc ataaaagcat
    22261 caggtaaaaa ttcatattgt ttatttgaag aagaactata tgtacagtga atttgaattt
    22321 agattttatg tgagagtaag atttatatat acttgtggct gtctgattta taaaatatgt
    22381 tactttgtat atttttatat gtttcagtaa ggttaattaa catgagcaaa ggtgtaagta
    22441 aatttatatt agtttaagtt tacttacacc tttataaaaa tacaaataat agattcattt
    22501 acattcttaa atgattaaag ctctttacct tctaaacatg cataacatat cgttataatt
    22561 ccagtaaata agactaaaat gcccattact aagattacaa tttcatcatc tattactcca
    22621 aatgttttta aagtaactta tttcgctttg tatcataata atttgtctct ttttacattt
    22681 taacttctgc tgagatttga gttagatatt cattagaatc tcgcacactt atatcatata
    22741 gaagatattc ttcataaaca aaatccccaa tacgtaaatt attctgatta aaaaaattta
    22801 ataacttttc ataagccttg taaatagttt cataactccc tttatgataa gcagttacat
    22861 aaagtccctt tggtttgatt gatacaggat atttcttgta ctctttatta acttttgtaa
    22921 ataaatatga atatgattca aagtctttat taattatatc ttcaccttta atgattgagc
    22981 caatcgtacc ataatcactc aattcatagt tgtccataaa gttaatacat tcatataaaa
    23041 aattttcttc ctcatctcct aaaaactgtt ctttatatgt aacaggtgtt ttgaccaagt
    23101 actcttcttc atgttctttt aaagtaatag tattcgcatc tatattacat gcacttttag
    23161 taaatgaaat agtagattcc attagagctt gaatattatt tagtttttca atttcatttt
    23221 taatatcaat ggttttcttt tctaaaagct caattagtaa attaggtgtt cttttgtcta
    23281 tataagcttt aatttctttt aaaggcattt ttacttccct aagtgaagaa attataccaa
    23341 aaatgctaag ttgatggtat gagtagtatc tatatccatt ttctttcttt aattctggtg
    23401 agaaaagacc tatatcatca taatggaaaa gtgttttttt attgattcca caaatctttg
    23461 caaattctcc agttgtaaaa taattttttg aaacttgact cataattata attcctcctt
    23521 gactatcctg ttacagtata gtttatcata gtattttgta aaggagtgtg attatatgaa
    23581 aaaattaatt cattttttaa aaccatatag agttttaatt gttgtagttt tgatatttac
    23641 gtttttacaa acacttggaa ctttgtatat accaacactt acagcaaata tagtaaataa
    23701 tggtgtagta aagggagata tagattatat tgtaaaaaca ggtttaatga tgatgatagt
    23761 agctggtata acagctttat cagctgtgtt agtttgtaaa gtatcagcaa atctttcatc
    23821 tggattttgt agagatatta gagaagcagt atttataaaa tcacaagatt tatcaataaa
    23881 tgattttaat aatataggta cagcatcaat gattactagg agtactagtg atattacttt
    23941 aataggtcag tctgtgttta tgtttattca attagtatta ccagcaccaa ttataactgt
    24001 ttctggtcta ttcttagcat attctataga taaagcaatg acaattatta ttgttgtagt
    24061 aatgttttta ttcatgcttt cagcattttt agttggtaaa aagcttataa agttgtttaa
    24121 aatgatgcaa ataaagatgg ataatatgaa tagggtgtta cgtgaggtag taactggagt
    24181 aagagttata agagctttta ataggagtcg ttttgaaaaa aagagatttg atagaactgc
    24241 aatagattat tctgaaacag caatttcaat aaataaaata tttgcagtac ttatgcctat
    24301 tgtaatgtta ataatgaatt taggtatagt aagtattatt tggtttggtg ggatgagagt
    24361 cagtaatgga aatatggaaa taggtcatat aatggcactt gttgaatatt gtatcttaat
    24421 tttattctat ttaatcatgg gagtaatggt atttatgtac atacctagag caggtgcatg
    24481 tgcagataga gtaaatcaga tattagatat agaacctgaa attgtagatg gaaatggtca
    24541 taaagatact gtctcagaga gaggacattt agtatttaaa aatgtaacct tttcatatgc
    24601 acaatcagaa gaacctgtgt taaataatat aacatttgaa gcaaaatctg gagaagtaac
    24661 tgcaattata ggaagcactg gctcaggcaa atctactatt gcaaatataa taccacgttt
    24721 ttttgaaatt caatctggag aaatatctat aaatggtcag gatattaaga agattcctca
    24781 aaaagaactt agagataaga ttggatttgt accacaaaaa gcttttttat ttagtggaac
    24841 aatagaagaa aatataaggt atggaaaaga agatgcaagt attgaagaag taaagcatgc
    24901 tgcaagtata gctcaagctg atgaatttat ttatgatatg gaagacaagt atgactcatt
    24961 tgtagcacaa ggtggaaata atttgtcagg tggtcaaaaa cagagaatat ccattgcaag
    25021 agcgttagtt agaaagcctg aagtatatgt ctttgatgat agtttttctg cattagattt
    25081 taaaacagat aaaagactta gaaaagcgtt gaaaaatgaa atcaaagatt catcagctat
    25141 aattatagca caacgtataa gtacaatcat ggatgctaat cagattatag ttttaaatga
    25201 tggaaaaatt gtaggtattg gaaaacataa agatttactt gaaaattgtg aagtttataa
    25261 gcaaattgcg gattcacaac ttagtaaaga agaacttgca taaaatgaag aattaatctg
    25321 atatttggag gtgtatgata tgagtgaaaa aagaatagat gaacaagata tagaagtact
    25381 tagttttgaa gatggcatgt cagcagaaaa agctaaggat gcaaaaaaaa ctactaagag
    25441 attattaaaa tatatggcaa aacaaaagtt taaaatatta attattttta tttcagtatt
    25501 gataagttca gcgcttacag ttttagcacc tatggttatg ggaaaagcta ttgaccaact
    25561 atttaatgga ataaaaactg cagtccaaac aggtacaaaa ttcagtgtga attttagtac
    25621 tatgggaggg atagtatcaa tacttctagg gttgtactta attagtgcag tatttatata
    25681 tattcaacag tatattatgt caggagttgc acaaaatcta gttcttagca tgagaaaaga
    25741 tttaagtgat aaactcaata aacttcctct taagtactat gattctcata aaaaggggga
    25801 aacacttagt atagtaacaa atgatttaga aaaagtagca gatagtttac aagaaggatt
    25861 aatgcaactt attacagcag tagttacagt agttggttct attgtaatga tgataagtat
    25921 aagtgtacca cttacaatag tatcagctat aacattactt gtttcattgg gtattactgt
    25981 tgtaattgca agaaaatcac aagaaagatt ctctgaaaat caaaaggcac taggagagtt
    26041 aaatagcaat atagaagaga tatttactgg tcaaatagtg gttaaagcat ttagtaaaga
    26101 aaaggatact ataagaaact ttaaaatatt aaatcaaaat ctttataatg caagtagaaa
    26161 agcacagttt tcgtcatatg caatatctcc aattattaga tttataaacc taattggcta
    26221 tgttattata gcagttgtag gtgggatatt tgcatcaact ggagcaatga ctcttggtag
    26281 tatacaagcg tttattcaat atgtaaatca agcatcagag cctactacag aaatatctta
    26341 tattgtaaat atgcttcaag cagcaatagc atctgcagag agagttttta cagttatgga
    26401 cgaggtggaa gagataaagg atgaagaatc ttcaaaggtt attagcatgc ctaaaggtaa
    26461 agtacaattt gagcatgtta aatttggata tagtgatgat tttattctta tgaaagatat
    26521 taatatcaat ctaaatgcag gagataagat tgcaattgtt ggacctacag gagctggaaa
    26581 gacaactctt gtaaatttac ttatgagatt ttatgagata caaggtggaa gaattactat
    26641 tgatggtgtt aatataaaag atttaaagcg tggagaactt agaactatgt ttggtatggt
    26701 tcttcaagat acatggctat ttaatggaag tataaaagaa aatattgcat atagtaagtc
    26761 agatgccaca atggatgaaa ttgtaagagc agcaaaatca gcaagagtag accattttat
    26821 aaggacttta ccacaagggt atgacactat attgactgag gatgcttcta atctatctca
    26881 aggtcaaaag caacttctaa caattgccag ggcaatactt tcagacccat cagtattgat
    26941 tcttgatgaa gcaacatcta gtgtagatac tagaacagag gtagaaattc aaaaggcaat
    27001 gaataacttg atgaagggaa gaactagttt tgttattgca catagattat caactattcg
    27061 agatgctgat ttaatattag taatgaaaga aggaactatt attgaacaag gaagccataa
    27121 ggaacttatt gaaaagaaag gattctatga agaactttat aacagtcaat ttacaagtga
    27181 atatgatgaa gatgttgtat gattatcaag ttttaggcaa ctaaatgatt gataaatatt
    27241 aagttaaata tagtaacatg taaggttata tggaataaga gtatttctct atttcatata
    27301 acctttttta tttatcaaaa taagataaaa tttcctttct aaattagatt gaagaatgta
    27361 tttatcaata taaaagtaaa ataatcatta ttattagtga agtaaatatt gtttgccaca
    27421 tatatgccat aaagatgcac ttattttacg gagatttaac aaagtagtgt aaagtatact
    27481 ctaaatataa aataaatcaa atagagttaa ataaattgag aggtgatata aatagtgaat
    27541 aaaaaattga actatagatt tgaaatgaag cacaaaataa ctgaagcaga tgtgttagct
    27601 ttaaaatcaa gactctatcc aataatgaag aaagatgaaa atgcatctaa agatggaaaa
    27661 tatcttatta ggagtttata ttttgatact cctgaagata aagcactttt ggataaatta
    27721 aatggcgttg caataagaga aaagtttagg ataagatttt ataataatga ctgctcttat
    27781 ataaggttag agaaaaaaat caaacattac aacatgacat caaagctatc aactaattta
    27841 accaaaaaag aagtaataaa tatactaaat aatgatattg aatttctaaa agagtcgaca
    27901 aattgtttat taagagaatt ttatttgaaa ttaaaatgtg aaagattaga ggcaaaaagc
    27961 atagttgatt acaatagaga agcttttatt tatccagttg gaaatgtaag agttacaatc
    28021 gatagtgata ttaaaacttc tgtaaattcg gtagatttat ttaataaaga tttgccaact
    28081 gtttcggtaa ttgatgagaa tatgactgta ttggaggtta aatatgatga atttatacca
    28141 gattttataa aggatttaat tcaaattaat aagactacct ctacagcagt ttcaaagtat
    28201 gcttcaagta gattatatat ttaagaataa aggctatgat ttatataaag atatacataa
    28261 ataagattaa aaaacaaata agttaaaaat aaaaaataaa taagttaaaa ataggaagga
    28321 tgattttaat tatgacattt aatgatattt ttaaatcaag ttttatagaa aacgtatcag
    28381 gtttttcatt tgtggacagt gctttagcac taggttcagc ttttttagta ggtttgttta
    28441 tatatatggt ctataaaaag acttatatgg gaattatgta ttctcgacca ttcaatgtgt
    28501 cgcttgtagc acttacaatg cttacaactt ttgtaatatt ggcagttact tcaaatgtag
    28561 ttttatcctt aggtatggtt ggtgctttat ctatagtaag atttagaaca gcaattaaag
    28621 acccaatgga tttagtattt ctattttggt cattgggttc aggtatagtt ttaggagcag
    28681 gtttgattcc cttagctgtc atgggttcaa taattatggg attgattcta atattctttt
    28741 caaataaaac aatatctgaa actccatata tattgatggt aaattgtaat aatgaagatt
    28801 cagaagatat agcaactgat aaaattaaaa aagtatttac taaatatcaa ataaaatcaa
    28861 aaagtgttac accagaaaaa ggaatagaat tagtgtttga agtaagaatg aaagatggag
    28921 aaacaagtct tataaatgac ttgagtcaag tagatggagt tactaatgca gttcttgtaa
    28981 gttataatgg tgactatgta gcatagagag gagtaatctt ttatgaaaga taaaaaattt
    29041 acccttctta tctcgattat gattatattt ttatgtgctg tagttggagt ttatagtaca
    29101 tctagcaaca aaagtgttga tttatatagt gatgtatata ttgaaaaata ttttaacaga
    29161 gacaaggtta tggaagttaa tatagagata gatgaaagtg acttgaagga tatgaatgaa
    29221 aatgctataa aagaagaatt taaggttgca aaagtaactg tagatggaga tacatatgga
    29281 aacgtaggta taagaactaa aggaaattca agccttatat ctgtagcaaa tagtgatagt
    29341 gatagataca gctataagat taattttgat aagtataata ctagtcaaag tatggaaggg
    29401 cttactcaat taaatcttaa taactgttac tctgacccat cttatatgag agagttttta
    29461 acatatagta tttgcgagga aatgggatta gcgactccag aatttgcata tgctaaagtc
    29521 tctataaatg gcgaatatca tggtttgtat ttggcagtag aaggattaaa agagtcttat
    29581 cttgaaaata attttggtaa tgtaactgga gacttatata agtcagatga aggaagctcg
    29641 ttgcaatata aaggagatga cccagaaagt tactcaaact taatcgttga aagtgataaa
    29701 aagacagctg attggtctaa aattacaaaa ctattaaaat ctttggatac aggtgaagat
    29761 attgaaaaat atcttgatgt agattctgtc cttaaaaata tagcaataaa tacagcttta
    29821 ttaaaccttg atagctatca agggagtttt gcccataact attatttata tgagcaagat
    29881 ggagtatttt ctatgttacc atgggatttt aatatgtcat ttggtggatt tagtggtttt
    29941 ggtggaggta gtcaatctat agcaattgat gaacctacga caggtaattt agaagacaga
    30001 cctctcatat cctcgttatt aaaaaatgag acatacaaaa caaaatacca taaatatctg
    30061 gaagagatag taacaaaata cctagattca gactatttag agaatatgac aacaaaattg
    30121 catgacatga tagcatcata tgtaaaagaa gacccaacag cattttatac ttatgaagaa
    30181 tttgaaaaaa atataacatc ttcaattgaa gattctagtg ataataaggg atttggtaat
    30241 aaagggtttg acaacaataa ctctaataac agtgattcta ataataattc taatagtgaa
    30301 aataagcact ctggaaatca aagtgatgaa aaagaagtta atgctgaatt aacatcaagc
    30361 gtagtcaaag ctaatacaga taatgaaaat aaaaataaaa ctacaaatga tagtgaaagt
    30421 aagaataata cagataaaga taaaagtgga aatgataata atcaaaagct agaaggtcct
    30481 atgggtaaag gaggtaagtc aataccaggg gttttggaag ttgcagaaga tatgagtaaa
    30541 actataaaat ctcaattaag tggagaaact tcttcgacaa agcaaaactc tggtgatgaa
    30601 agttcaagtg gaattaaagg tagtgaaaag tttgatgagg atatgagtgg tatgccagaa
    30661 ccaccggagg gaatggatgg taaaatgcca ccaggaatgg gtaatatgga taagggagat
    30721 atgaatggta aaaatggcaa tatgaatatg gatagaaatc aagataatcc aagagaagct
    30781 ggaggttttg gcaatagagg aggaggctct gtgagtaaaa caacaacata cttcaaatta
    30841 attttaggtg gagcttcaat gataataatg tcgattatgt tagttggtgt atcaagggta
    30901 aagagaagaa gatttataaa gtcaaaataa gattaaaatt gaatataaat aattttaata
    30961 ctgttaagtc taaatattta cttagcagta ttttttagaa aataatgtat ttggttacta
    31021 atataaggta ataaattgat aaaaattaaa tacagcatat taataataca taataaagta
    31081 ataaaaaaac tgtttaagta taataactta tatttagaca gtttttttca tttataaata
    31141 atataaatga aattgaaaac atttgaagga tagaaattgg ttggataaat gtataaatta
    31201 cttcaccaaa aaataatgac aaaaattgta actaagttgt aataaaatgg tagttattta
    31261 gcataaaata atgtatcata gaagtatatt ataaaattaa taaaataaga tttttgtaag
    31321 aaaactgctg gcttttgata tttagagtga gtagttaaat tatgtttagg aggggtagaa
    31381 tttgttgcaa aaagtattat ggaaattttt aaagctagta gtcggtttat ttatatgttc
    31441 agttggtata gtattgacga taaattgtaa ccttggatta tctccctggg atgtatttca
    31501 tcaaggactt tcaaatcata taggaataac tataggaact gcttctataa ttgtaggctc
    31561 tatagtagtt attgctgatg tcgttcttgg agaaaatgta ggatggggaa ctgtttttaa
    31621 catgctacta attggatttt ttatggattt acttttatat agtaatttaa ttccagaggc
    31681 tgataattta tttgtaggaa taattatgtt gatattagga ttggttcttt tgtctgttgg
    31741 aatggtattt tatatgggaa gtggtttagg aagtggacct agagatggtt tgatggttgc
    31801 aattcaaaag aaaacaggaa aatcacttaa attaataaga ggtactatag aagttggagc
    31861 attaatagta ggatttttat taggtggaaa agttggtata ggaactataa taagtgcatt
    31921 tggattgggg tatttcacac aaatggtatt tggcttattt aaattagatt gtagtaagat
    31981 taaacataga tttatagtag atgatataaa atttatcaaa gtgtatatga gtggtgagaa
    32041 aaaagctgct gttcaaaata tagttaagta atttaagaat aaattcaacg aaatgttaag
    32101 tattttaaat taacattttg tttagataga taaattgaat attaaaagaa tattaaaata
    32161 tgaggattgc tagaacacta taaaatatag atttctagta attttttatt tgttcaatat
    32221 ttttttagta ctagaagtct tattctctat ttaatgaatg aattaattta taattagaga
    32281 tactaaactt tatagatatt aatataaaca cctttaaact gtgtattttc caaagccttt
    32341 aaaatgcttg tatttattct aaataaaaaa ataaaaaaat gtcaaaaaaa ctcttgtatt
    32401 atatccaaat tttatatata attacctatg ttgtttagaa attttaaact caatgtttag
    32461 taaacaaaat gtttagtaaa ataaaacaga aactaggagg tgcaacatgg atataggtga
    32521 aaagataaaa agattaagaa cagaaaaaca actgacccag gaagagttag ctaatagatg
    32581 tgaattgtca aagggattta tttctcaatt agaaaataac ttaacatcac catccatagc
    32641 tacccttata gatatacttg aaatactagg tacaaattta agggaatttt ttaatgaaat
    32701 agatgatgaa agaatcagtt ttacaaaaga agatatgttt gaaactgaag atgaagattt
    32761 aaaatataaa ttaaagtggt taattccaaa ttcacaaaaa aatgaaatgg agccaataat
    32821 aataacattg tatccaggag gacagtataa agaagaaaag ccacatgaag gtgaagaatt
    32881 tggatatgta ttagcaggtt caatatatgt ccatataggc gaaaagaaaa ataaagtaaa
    32941 aaaaggagag agtttttact ttagaccgaa agcaaatcac tatatatcaa acgagggcaa
    33001 aactacagca aaagtgattt gggtaagtac gccaccatca ttttagaata gaggtgttta
    33061 ataattggta gaaaatataa tagaattaag ggatatatct aaacattatg aagaacttac
    33121 tgttttagat aatttcaatt tagatataaa gaaaaatgag tttctaacat tgcttggacc
    33181 aagtggatgt ggaaaaacta caacattaaa aataatagca ggatttgagt atgctgatga
    33241 tggtaaagtt ttatttgaag gtaaggagat aaataatttg ccaccatatg aaagacaagt
    33301 aaacacagta tttcaaaaat atgctttatt tccacacatg gatgtttatg aaaatgtagc
    33361 ttttggatta aaaattaaaa agactccaaa agatgtaata gatgctaaag taaaagaggt
    33421 tttgaaactt gttgcactag aaggttttga aagacgacaa gttgaatcat tgagtggagg
    33481 acaacaacaa agaattgcaa tagcaagagc tctagtcaat gaaccaaagg tattattact
    33541 agatgaacca cttggagcac ttgatttaaa gttaagacaa gagatgcaaa tagaattaaa
    33601 gagaatccaa aaaaaattag gaataacatt tgtttttgta actcatgacc aagaagaggc
    33661 tcttactatg tcagatacta ttgttgtaat gtataagggc aaaattcaac aaatgggaac
    33721 accacaagat atatacaatg aacctaaaaa ttcatttgta gctaagttta taggtgaaag
    33781 taacatattt gatggaatta tgatagaaga ttataaagtt aatttctgta atagagattt
    33841 tgagtgtgtt gataaaggat tcgaaaaaaa cgaaaatata gaagtagtaa taagaccaga
    33901 agatataaaa atggtaaagc cagaagaagg aatgttaaag gggaaagtta catctacagt
    33961 gtttaaagga gtgcattacg agatagaatt gaatgaaaat ggaagattgt ggatacttca
    34021 taatactaaa aatgctgagg ttgggactga actaggcatg gatatatatc cagaagatat
    34081 ccatataatg agaaaagaga ataactaaga tgaaaagaaa atctttttta gcatatccat
    34141 atgtagtttg gagtgcgata tttgtaataa ttcctttaat attagtagta ttttttagtt
    34201 ttacaaaaga atcaggtgga ggttatgcct ttacacttga gaattataaa gaagtaattg
    34261 accctatata tatgaaagtt tttgggcgtt ctattctttt agcaggagga gctacattaa
    34321 tatgccttat agttggttat ccagtggcat atataatttc aaaggctcgt gtaagtagaa
    34381 gaggttcatt aatattattg tttattcttc ctatgtggat gaactttctt ttaagaacat
    34441 atgcatgggt tgctatatta ggaaaaaatg gacttttaaa tacattttta ggttggtttg
    34501 gaatacaacc attagcaatt ttgtatacta attttgctat acttttaggt atggtgtata
    34561 actttttacc atttatggtt cttccaatat atacagcact atcaaagatg gataatgatt
    34621 tgataaacgc tgctcatgac ttgggtgcaa ataatatgac agtttttaga aaaattatat
    34681 ttcctttgag tttgccaggt gtaatgtctg gaataactat ggtatttatg ccagcagtca
    34741 ctacttttgc aatatcaaga ctgttaggtg gaggtaaaat aatgctggtt ggagatttga
    34801 ttgaacaaca gtttacagtt gttggagatt ggaactttgg ttcagctata tcgatattta
    34861 tgatgatagt aatattaata tctatgtcta taatgtcaaa atttggagat gaatctgaca
    34921 aagaaggcgg tgggttacta ttttaaaatt aaagaaaata acttctaata tatatttagc
    34981 attagtattc ttatttttgt atgcacccat atttgctttg gtactatttt ctttcaatga
    35041 ttcaaaatca atggcaagat ggaatggatt tacatggaag tggtatggtc aattacttca
    35101 aaatgagagt ataatgagtg ctttatatta tactattgta atagctatat tagcgtctgt
    35161 aatatctaca atagtaggaa ctataagtgc tataggaata cataagatga gaggtaagag
    35221 taagaaatta atactaaatg taaactatct tcctatttta aatacagaga tagttacagc
    35281 agtagctctt atgagtctgt ttgtatttgt aaaaatggaa tttggattta caacaatgct
    35341 attggcacat ataatgtttt gtttgccata cgttatactt tcagttttgc caaaaatgaa
    35401 gcaattacct gataatatag aagatgcagc tatggacttg ggagcaacac ctatatacgc
    35461 tcttagaaaa gtaattttac cacaaataaa accaggtata gtttctggat ttttaattgc
    35521 atttactatg tcaatagatg actttataat aagtttcttt aatgctggaa atggtgtaag
    35581 caatctttct atagaaatct atggaatggc tagaagaggt ataaaacctg aaataaatgc
    35641 attatcaaca ataatgtttg cagtggttct aggtttactt ttactagcta ataaaaaaga
    35701 atctatagta agggggatta aataatgatt aagtttaaaa aagcaatatc ccttatagct
    35761 gtaggtatga tgacagttac tttatttaca ggatgtaata aagcaactga ttctacaaaa
    35821 gttttaaatg tatataatgt tggagattat atagatgagt ctcttataga taagtttgaa
    35881 aaagaaacag gaatagatgt tcaatattca acttatgata ctaatgaaat gatgtatcaa
    35941 aaagtaaaga gtggtagtac acactatgat ttagtatttc cttcagacta tatggtagag
    36001 aaaatgaaaa atgaaggtct tttggaaaaa ttagatttta agaatatacc taatatgaaa
    36061 tatttagata agagtttttt aaatcctgta tacgacaaaa ctaatgaata tagtgttgca
    36121 tatatgtggg gaactcttgg aatattgtac aataagaaag aagttaaaga ccctatggat
    36181 agctggaata tactttggaa tccaaagtat aagggaaata taatgatgtt tgactctgtt
    36241 agagatacta tagggataac tcttaaaaaa ttaggatata gcatgaatag tgtaaatcca
    36301 aaagaaataa atgaagctaa aaatctttta atgaaacaaa aggatttagt attggcttat
    36361 gtaaatgatg aaggtaagga tagattgctt ggtggagaag tggcaatggg tatgctgtat
    36421 tctggagatg cagttacact aatggagcaa aatcctgacc ttgattatgc tatacctaaa
    36481 gagggaacta ataaatgggt agatgctatg tgtgtaccta caacagcaca aaataagaaa
    36541 gaagctgagc tgtttataaa ctttttatta gacccagaga atgcaaaagt aaatgctgag
    36601 tacattgggt attcaacacc taatacagga gcactaaaat tattagaccc agaaataact
    36661 cagaatcctg ttgcatatcc atctaaagaa gtacttgata aatgtgaaac ttttgaagat
    36721 attggtgaaa atataaaatt atatgacaag gcttggatag agttaaaatc aaaatagggt
    36781 cttgttgttt taagtaaatt tacttaataa atacttaata aaactgttct tttttagaaa
    36841 atttattttc taattattga acagttttat tttttgttta agttatattc atatgcaatt
    36901 taaatagttt attaataaat gaaaacttat ttgtataatg actttaataa taattagaaa
    36961 aaatgggcaa cttaatagaa taattggagg tgtaaattta taaaaaatga ataggataaa
    37021 taaaaaaata gatttattaa tattggcttt gctcgttttt atatttttaa ttgttggaat
    37081 agtaatacta tcgattagga cagaaaataa attgaatgtg ttcataatgt tagcaataat
    37141 tttttttata ataatgttaa cttatttatc aaattcagta gtggggttga taacaagttc
    37201 aataattata tttatgtata catcctatat tttatataac aatattactc acaatatgga
    37261 tgttgaattt atatcatata tgtggataat agcaacacca gtatcttcaa ttataatggg
    37321 taatctgaac aaaagcataa atgaattgca aaatacaaat aaaaaactat ctgaacagta
    37381 taaggaatta gtgactattg atagtgaaac tggacttaga aatttaaaga ttttttataa
    37441 tgatgtaaat atggagataa gtaaatctat tagacataat actgattttt ctcttatgat
    37501 agtaaaatta ccatactacg gaaacctaca gactatattt ggtgaaaaca aaactaataa
    37561 aatagtaaaa tatattggtt caaatattat agaatgtaca agaaatgaag atataattta
    37621 tagtttacaa aaagatatga tagggatact aatgccaaat acatctttgg aaggttcaaa
    37681 agttgttaaa gacagaataa agaaaaggat aaaagaatta aatttagatt taaacaatag
    37741 gggaaaatat gtaaatatag atgtcaaaat tgcattttta caatataaga gtagttttgg
    37801 agatagtata aattttaaga atatagttga agaggaactt caatatgatg tttaaaatat
    37861 ttaataaaag tctattaaaa ttagtagttg tattatcaat agtactgaca atatcaaatt
    37921 ttagcttatc ttcatatgca tcagaaatta cttccagcac agataaggta ttaatcatat
    37981 atgattctaa gaaagaaaca gcttataata gagatatttt aaatattatg aggactctat
    38041 tgggaagatt tagttcagat atagagctat taaaattgag taactatgat ggtgagataa
    38101 ataaaaatta ttacagtcat atatttattt taggaatcaa tgaaaactca tataataatg
    38161 ataaaaacac taaaaacttg attagttcct taaattcata taaaggaact atatgttggt
    38221 taggatatgg tatagaaaat cttttggaac ataaaaaata caatttagac tatgtaggaa
    38281 aaacaaacaa tatagttagt gtaaattata gaggtaaatc atataacttg gatgaacatt
    38341 atgtttttaa cattgtagaa tcaaaagata caagtaataa agtaatagga agtattaatg
    38401 ataccttaaa taagtatcca tatataataa atgataaaaa tcttttttat gtatcaaaat
    38461 tagatttaga tggagtttta ttttatatat tttgcgattc acttaatgat atttttaata
    38521 ttaagacgtt tgataaagga cgtatatttg taagaataga agatgtacat gcttttagag
    38581 aaccaaaaaa tcttgtagag atagcggatt atttaagtag taagaatata ccatttacga
    38641 ttgcattaat accagcatat gtaaatccta aaaatcataa ggtaataact ttatcagaaa
    38701 gccctgaaat tgtcaaagca ataaaatata tgcaagataa aggtggaact gtgatattgc
    38761 atggatatac tcatcaatac aagaaagaag aagtttctgg agaagggtat gaattttggg
    38821 atggtaaaaa agatgaaccc ttaaaagaaa atatgaagat atttgtcaaa gatagagttt
    38881 tgaatggtct tagagtatgt atagagaatg gaatttatcc tcttgcattt gaagcgccac
    38941 attatgcaat ggaatcagag ggatataaag aattaaaaaa atatttttct acttatatgg
    39001 ggcaacatca aaataatgat aaaaaattta gcacaaatac atatccttat attattaggg
    39061 atacagagga gtttaatata tttatacccg aaaatttagg atatatagac ccagaagata
    39121 agtttacttt tcaaaatata aaagaaaatt taaataaatt atccattgta agaggtttta
    39181 gtggagggtt cttctttcat tcatatttaa acatagagta tctaaaaaac acaatagaat
    39241 atttagaaaa acagaatata gaatttatga atttaagaga ttttaataat tgggtaaaag
    39301 ttgatgaaat acaaataaga aataatggag atgaaattat agtcaactat gataaggact
    39361 tagatgaaat aactaaaagt gatactagat ttaaatccat atccaatatt agtaaagttt
    39421 taatattcat agtatctata agtgttttaa tatttgtgat tatatttata tattttaaga
    39481 gaatagataa aaagaaattt ttaaaataaa tgattttata ttagaatcaa tattttaaaa
    39541 tattttagga gaaatttatg agagatttaa atgtatcaga gtatttgttc gtatttagtc
    39601 tgtttagtat atggtcttta ttgcttataa atattatttt agctatgggt ggatatatat
    39661 tttattttaa aaactttgat aaagaaataa aagaaattga tgaatatcct atgatatcaa
    39721 tacttgttcc tgctcataat gaagctaaag tgataggtag aactgtggag tctttgctat
    39781 tactcaatta tcctaagagt aagatggaac ttattgttat aaatgacaat tcttctgata
    39841 atagtaaaga aattttagaa aatataaagg ataggtacaa caattataat ttcactataa
    39901 taaatacaga tagtttaaca ggtggaaaag gaaagtctaa tgcattaaat attggatata
    39961 cgatttcaaa aggagatttt atagctgttt atgatgcaga taacacacca gataaaaatg
    40021 cacttagata tctagtacaa actatagtaa tgaatgatga attaggtgct gttattggga
    40081 aatttaggac tcgaaataaa aataaaaacc tattaaccaa atttataaac atagaaacat
    40141 taagctttca atggatgtca caagctggta gatggcaact ttttaatcta tgtacaatac
    40201 caggaactaa ctttatattg aggagaagta tcatagaaga aattggagga tgggatagta
    40261 aagcaattgc agaagataca gaaataagtt ttaggatata taaattggga tacaagataa
    40321 agcttgtacc acaatctata acatgggaac aagagccaga aactgttaag gtgtggataa
    40381 agcaacgtac tagatgggca aaagggaata tatatgtatt aatgaaatat ataaaaaata
    40441 tatttaaaca gggaagaaat aagattgtgt ttgatatagc ttactttttt tctgtatatt
    40501 ttctattttt aacatctgta attatatccg atattttatt tgttttaagt atatctaaac
    40561 tagtagaaat ttctattcca attaactttt ttctaatatg gatactatct tatcttttat
    40621 ttattataga agtgagtata agccttacta tagaaaaagg tgaagcaaca attgaaaata
    40681 tattcatagt ggcaataatg tattttacat actctcaact atggctgttt gtagctataa
    40741 aaggaatgat agagtatcta aaagatataa tttttaaaag agaggttaaa tggtataaaa
    40801 cagaaagatt ttgattcatg tacaggagga attaacatga aaaaatttat tatatcaatt
    40861 atatctttag tattgttttt ttcaaatata tcacttatat acaaagtaaa tgctgatgag
    40921 actaaagtta aaaattataa gtttgaaaga gatataacta ttgatggtgt tataggaagt
    40981 aatagtactt tttttgaagt aaataaaaat tgggatattg aagaagtttt attgcatctt
    41041 aattttagta agagccaaat attaaatgga gatgtttcaa gtttaactgt attgataaat
    41101 aatgtaccaa taaaatctat taaattaaat gctaagacaa attataaaaa tacattagaa
    41161 gtattagttc caaaagatta tataattcaa ggatataatg aaataaaaat aaaaacatac
    41221 aaaacaatat ctgataaaat ttgccaagat gattccaata ctgggaactg gatggttatt
    41281 cacaaagaat catatatatc aattagatat aagcagaaaa aggtggaaaa ctcaataaat
    41341 gaatatccgt atccatatgc agaaattgaa aataatcata aattagatac aactatagta
    41401 gtaccagata atatgactag aggagagact acagcagtgt ttaatttggc atcagcattt
    41461 ggaaaaataa caaaaaatga tgatttaaag ttagatgtta aattatattc agaaatgaaa
    41521 aattggtcag atgataatat tatatatatt ggtaaacctg aaaatacagc agaagaaatt
    41581 ttggatattt taagtataaa ggaacaaacc ctattatctt caaactgtat aatcaagcaa
    41641 gtagattcac catacaataa gaataagaag atgatggtgg taattggtag taatgaagat
    41701 gatttaataa aggcatcaaa tttattaata gaaaatagac tttctaacca agttttatca
    41761 tcatcagtac ttgtaaacaa agagactaat ataaaaatta atagggaaca aaaattaaat
    41821 ttaggtcatt taacattaaa agacttagga tattctgact ttttgttgga aggagcattt
    41881 aatcaacaag ctttgtttga tgtcaaaata ccaacgggta aggtattaga tgatggttca
    41941 aagattatct taaatttaag atattctgac aatttagatt ttgaaaaatc tttagttaca
    42001 gtgtctataa atgatgttat tgtgggaagt aaaaaattgg ataggtcaca ttctaataat
    42061 gataagttag aattaaaaat acctaaagat atagataata aaaactatta tcaagttaaa
    42121 ttgacattta atttgagtat aaaaaattcg aattgtgtta ctagagagag taataatcct
    42181 tgggcatatg tatcaaataa ttcttatttg gcgttatcta tgaaagaaaa tgaaacctta
    42241 tcttttgaaa attatccgta tccatttgtt agagatgatg aatttaatga tttaactgtg
    42301 ataatgccag attattcagg gtcacaagct atgacttgga tgtttagatt aggtgttact
    42361 ttaggtgcga atataaattc acataatgga aatataaatg taattagagg aaaagaattt
    42421 agtgacaagt ataaggatac taatattgta gtttttggag tgccacataa taattctgtt
    42481 ataaagatgt taaataataa tttaaatata aaattcgata aaaattactc caattttata
    42541 tcaaatgata agataagctt tattgatgat tatggaaaaa atatttctac cattcaatta
    42601 ataaaatcac catataataa tcaaaaaaat atcatggtaa ttagttcaat gaatgaaaaa
    42661 aacttatatt taggaatgga ttatttgttg aataaaagta aagttaatga tttaaaagga
    42721 gatacattga taattgatga atacggagag gtagaagatt tagcttataa tttaaaatct
    42781 aagaaagaag taaaagattc aa
//
LOCUS       DAEMGG010000020        42619 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEMGG010000020 DAEMGG010000000
VERSION     DAEMGG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.020"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            176..1252
                     /locus_tag="KPK24_001061"
     CDS             176..1252
                     /locus_tag="KPK24_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2298766.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1355..2197)
                     /locus_tag="KPK24_001062"
     CDS             complement(1355..2197)
                     /locus_tag="KPK24_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG2298767.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2381..2953
                     /locus_tag="KPK24_001063"
     CDS             2381..2953
                     /locus_tag="KPK24_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2298768.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3116..3388)
                     /locus_tag="KPK24_001064"
     CDS             complement(3116..3388)
                     /locus_tag="KPK24_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298769.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3502..4374)
                     /locus_tag="KPK24_001065"
     CDS             complement(3502..4374)
                     /locus_tag="KPK24_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2298770.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4670..7777)
                     /locus_tag="KPK24_001066"
     CDS             complement(4670..7777)
                     /locus_tag="KPK24_001066"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG2298771.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGAKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7870..8102)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8277..8804)
                     /locus_tag="KPK24_001067"
     CDS             complement(8277..8804)
                     /locus_tag="KPK24_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG2298772.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8839..9624)
                     /locus_tag="KPK24_001068"
     CDS             complement(8839..9624)
                     /locus_tag="KPK24_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG2298773.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYERAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9634..9891)
                     /locus_tag="KPK24_001069"
     CDS             complement(9634..9891)
                     /locus_tag="KPK24_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG2298774.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9910..10368)
                     /locus_tag="KPK24_001070"
     CDS             complement(9910..10368)
                     /locus_tag="KPK24_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG2298775.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10408..11112)
                     /locus_tag="KPK24_001071"
     CDS             complement(10408..11112)
                     /locus_tag="KPK24_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG2298776.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11122..11757)
                     /locus_tag="KPK24_001072"
     CDS             complement(11122..11757)
                     /locus_tag="KPK24_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298777.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVNTLVLKNEKVDAK
                     IHVKGSIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IIVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12138..13178
                     /locus_tag="KPK24_001073"
     CDS             12138..13178
                     /locus_tag="KPK24_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2298778.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13641..14996)
                     /locus_tag="KPK24_001074"
     CDS             complement(13641..14996)
                     /locus_tag="KPK24_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG2298779.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15022..15291)
                     /locus_tag="KPK24_001075"
     CDS             complement(15022..15291)
                     /locus_tag="KPK24_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG2298780.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15581..17551)
                     /locus_tag="KPK24_001076"
     CDS             complement(15581..17551)
                     /locus_tag="KPK24_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298781.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGEDFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENNNNENDNSD
                     EKQPNTPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLS
                     SKSVFGTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIF
                     LFCILTPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17734..19209)
                     /gene="spoIVA"
                     /locus_tag="KPK24_001077"
     CDS             complement(17734..19209)
                     /gene="spoIVA"
                     /locus_tag="KPK24_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG2298782.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19381..20676)
                     /locus_tag="KPK24_001078"
     CDS             complement(19381..20676)
                     /locus_tag="KPK24_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2298783.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSVINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASVA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENATKVS"
     gene            complement(20860..22269)
                     /locus_tag="KPK24_001079"
     CDS             complement(20860..22269)
                     /locus_tag="KPK24_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2298784.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(22321..23460)
                     /locus_tag="KPK24_001080"
     CDS             complement(22321..23460)
                     /locus_tag="KPK24_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2298785.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            23779..24429
                     /locus_tag="KPK24_001081"
     CDS             23779..24429
                     /locus_tag="KPK24_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2298786.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAE"
     gene            complement(24586..25605)
                     /locus_tag="KPK24_001082"
     CDS             complement(24586..25605)
                     /locus_tag="KPK24_001082"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG2298787.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNIIISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(25636..26250)
                     /gene="plsY"
                     /locus_tag="KPK24_001083"
     CDS             complement(25636..26250)
                     /gene="plsY"
                     /locus_tag="KPK24_001083"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG2298788.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(26285..27604)
                     /gene="der"
                     /locus_tag="KPK24_001084"
     CDS             complement(26285..27604)
                     /gene="der"
                     /locus_tag="KPK24_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG2298789.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(27638..28966)
                     /locus_tag="KPK24_001085"
     CDS             complement(27638..28966)
                     /locus_tag="KPK24_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG2298790.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(29204..29404)
                     /locus_tag="KPK24_001086"
     CDS             complement(29204..29404)
                     /locus_tag="KPK24_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298791.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(29406..30338)
                     /locus_tag="KPK24_001087"
     CDS             complement(29406..30338)
                     /locus_tag="KPK24_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG2298792.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(30383..31315)
                     /locus_tag="KPK24_001088"
     CDS             complement(30383..31315)
                     /locus_tag="KPK24_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG2298793.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(31370..32659)
                     /locus_tag="KPK24_001089"
     CDS             complement(31370..32659)
                     /locus_tag="KPK24_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2298794.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(32688..33380)
                     /locus_tag="KPK24_001090"
     CDS             complement(32688..33380)
                     /locus_tag="KPK24_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2298795.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(33395..34156)
                     /gene="pgeF"
                     /locus_tag="KPK24_001091"
     CDS             complement(33395..34156)
                     /gene="pgeF"
                     /locus_tag="KPK24_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG2298796.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(34153..34614)
                     /gene="nrdR"
                     /locus_tag="KPK24_001092"
     CDS             complement(34153..34614)
                     /gene="nrdR"
                     /locus_tag="KPK24_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG2298797.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(34636..34899)
                     /locus_tag="KPK24_001093"
     CDS             complement(34636..34899)
                     /locus_tag="KPK24_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG2298798.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(35003..35776)
                     /gene="sigG"
                     /locus_tag="KPK24_001094"
     CDS             complement(35003..35776)
                     /gene="sigG"
                     /locus_tag="KPK24_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG2298799.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(35868..36590)
                     /gene="sigE"
                     /locus_tag="KPK24_001095"
     CDS             complement(35868..36590)
                     /gene="sigE"
                     /locus_tag="KPK24_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG2298800.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(36615..37487)
                     /locus_tag="KPK24_001096"
     CDS             complement(36615..37487)
                     /locus_tag="KPK24_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG2298801.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(37778..39052)
                     /locus_tag="KPK24_001097"
     CDS             complement(37778..39052)
                     /locus_tag="KPK24_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG2298802.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(39336..40493)
                     /gene="ftsZ"
                     /locus_tag="KPK24_001098"
     CDS             complement(39336..40493)
                     /gene="ftsZ"
                     /locus_tag="KPK24_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG2298803.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(40683..41036)
                     /locus_tag="KPK24_001099"
     CDS             complement(40683..41036)
                     /locus_tag="KPK24_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG2298804.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(41135..41809)
                     /locus_tag="KPK24_001100"
     CDS             complement(41135..41809)
                     /locus_tag="KPK24_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG2298805.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(41814..42512)
                     /locus_tag="KPK24_001101"
     CDS             complement(41814..42512)
                     /locus_tag="KPK24_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG2298806.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
BASE COUNT        13320 a         7708 c         4620 g        16971 t
ORIGIN      
        1 attttcacaa taaaaagtca ttttacttcc gtatttaatg caaaagtaga aattaaactt
       61 aatataaaaa atatataaca aatttttttt atatttctag aagaaaagta tactcttttt
      121 tgtttaataa tgctaaaatt gatttgtatt gaattttgat taggaggaaa ttattatgaa
      181 agctcaaaga ttaaatgctg tacttgaaca aatgaaaaaa gatgatattt ctcaaatgtt
      241 agtaagtgac cctacttcta tattttatct tacaggagta ctaatccatc caggcgaaag
      301 actattagca ttatacttaa atttaaatgg caataataaa ttatttataa atgaattatt
      361 cccagtttca gaagatttag gtgttgaaat ggtttggttt aatgatactc aaaatccagt
      421 agaaataata actgaacata tagacaaaaa tgctactatg ggtgttgata aaaactggcc
      481 agctagattc ttattaaact taatagaatt aggtggagga agtaagtttg taaattcatc
      541 ttatataata gatactttaa gaatgtgtaa agatgaagaa gaaaaagagt taatgagaat
      601 agcttcaaaa ttaaatgaca aggctatgga acaacttaaa gctactgttt ctggtgaact
      661 tacagaaaaa caacttgttg gtaaattatc taaaatatat gaagacctag gtactgatgg
      721 attttcattt gaccctatta taggttttgg acctaatgga gctaatccac atggtgaacc
      781 tggaaatgca cttgtgaaac caggagatgc aataatctta gatataggtt gtataaaaga
      841 taactattgt gctgatatga caagaactgt attctataaa gaaatacctg aaaaaggtag
      901 agaaatattt gaaatagttc ttgaagctaa taagagagct gaagctattg taaaaccagg
      961 tgttagattc tgtgatatag atgctgctgc tcgtgactac ataactgaaa agggatatgg
     1021 acagtacttc actcatagaa ctggtcactc tataggactt gaagttcatg ataaaggaga
     1081 tgtatcttct ataaatacag atactgtaca accaggaatg atattctcta tagagccagg
     1141 tatatattta ccaggtgagt tcggagttag aatagaagat ttagtacttg ttactgaaga
     1201 tggttgtgag atattaaaca aacatgacaa agaaatatgt gtagttggtt aattctaaaa
     1261 tcaatttgat aaaattaaaa agccatataa aaatgtttta aacattttta tatggcttta
     1321 atttctcaat ctatgattta tttcaatcca ttatttatat tgcagttatt aagattgcac
     1381 cctttgaagt atatgtcccc atcactgaac caatttcaca gattacaaca tctttgtatt
     1441 ttacatcctt ttccataact tcccttacct tttgagcctt ttctgggcaa ttagcatgtc
     1501 ctataaataa aaccttttct tctttatctt taatttttga ctctactaaa gtaatcagct
     1561 ttttcaaact gtttccttca cctcttgcct tatctatagg agttacaacg ccatctgcaa
     1621 cttgtattat cgccttaaaa tttaaggtat ttataatttt tcctgctatt ggatttattc
     1681 taccaccctt aatagcattt tctaaagttt caagtgttcc aaaaaatgga ggattatctt
     1741 tatatttatt tattattgaa acaatctctt caatttcttt tccttctttt attaatattg
     1801 ctgcttttaa cactaattgt cctattccaa ttgacccact aagtgaatct ataacctcaa
     1861 ctttcttgtt atttccttcc ttttcaaaca tatcttttgc taaaagtgca ctattataag
     1921 tcccagataa tcctgaactt atattaatca ctaatacact ttcttcctga caatggtatg
     1981 cttctaaaaa tttatcagga gaaggacatg atgtttttgg tagtacttta ctctcactca
     2041 tttttctata gaaagtttca ttatctattt cctttccaga tatataactt tcttctccaa
     2101 aagatacatt taaacctaca acttctatgt tgtatttttc tattatatca tcaggtaagt
     2161 cacatgcact gtccactatc aattttattt tatccatatt aacctcacta taagatatta
     2221 ttttatttat tcagtcggtc gtacgaccga tttaattatt attttatata ttgaatgaaa
     2281 aaaagtcaat agatatagat tctcgcttta ttgacaataa atatccataa gcttataatg
     2341 ttattaattt gttataaata tacatggaaa ggtaggtttt atgaaaacaa ataatatgaa
     2401 acaggctatt ttatcatctg caaccaattt aatagccaaa aatggagttc aaaatactag
     2461 ccttgctgat attgcaaaag atgtaaatat aagtaaaggt actttgtatt atcactacgc
     2521 atctaaagat gatattatat atgatattgc ggatactcat ttagaagtta taactagtgc
     2581 tatactaaac tgtgtaaaaa atgtcaaatc aaaaaattct caaatagaaa tggttaactt
     2641 aatcttagag aaaatatcta ctatagaaag tcgtggtagg gtacatatgt atcttatttg
     2701 tgaggcaatt acttcaaata atgatttaaa ggaaagaata agattaaaat atattgaatg
     2761 gagaactact cttaaatctg aaattgttga atcactagaa aaatataata gtagcgaaaa
     2821 tgaacaggat gctgaatcat tttctttttt actcatgtca atagttgatg gtttagttgt
     2881 ccaaagttta ttaaaaacag aaaaaatacc ttatgaaaat atagcttctt ttttagttaa
     2941 tcattggatt taaacttaat acttagataa ttaataaaag tgaactattt aagttatcta
     3001 ctttaacagt tcactttctt atttattctc aataattttt acttaaaaca ttcaattttt
     3061 atttaaacct tcatggaatc acaaaataaa aatctcttat aaatttaaaa tgtttttatt
     3121 tatgttcatg tgtatgccca tctgaatgtt catgaccatg tgtatgtgta tgctcatggc
     3181 tatgctcatg agtacctgaa aaaccatcat catgattatg gtcgtgacct acaccattta
     3241 catgctcatg ttcatgccca tgactgtgag aatgttgatg tgaatgttca tggttatctc
     3301 ctccacagca tccttctcct ccagtcccac aacacccact tgtatgtgga ttatgatgat
     3361 ttcttactac aaatctctct cttaacataa ttcttatacc tcctagtata ttattgagta
     3421 cttcatgtac cccattaact acagtataca tctaactagg tataatctca atacatatgg
     3481 cattttttaa catatataat cttaaagaaa tttatcctta aaacctccac cttttacttc
     3541 ttcaacgtta ttgacagaaa taaaagcttc actatccata tcataaatta aatctttaaa
     3601 tttacctatt tcatttgcag atattataca ataaatcatc tttttcttat tttgtgtata
     3661 tgcaccttct gcctccaaaa aagtgactcc tctacccatt ttattcatta tcactttgga
     3721 tatttcatca tatttatttg atattaaaag tatagatttt tttctatcaa agcaatcttt
     3781 tcctatatcc atagtaaaag aaattatata cattgtaatt aaagtgtaca tcaccaattt
     3841 agctccaaat aaaaaagctc ctgcacatac tacaaaaaaa ttaataaata aaaatgtatt
     3901 tttcataggt atatcatact ttcttttcat tatggctgct attatatcaa gtcctccagc
     3961 agtagctctt gctttaaaag ttaaccccat acctatacca gttaatgcac caccataaat
     4021 acactgtagc atagtatcat ctattgggaa atatttagca atatcttgtg ttagccccag
     4081 tgctagagaa aatagtaaca tattgacaaa acttattagc aaaaatttat tatcaaaata
     4141 ctttctagct ataataaata ttggtatgtt catcaagaaa cttgatattc cctgattaat
     4201 accaaataat ttgtataata taacacaaat tccagctact cctccactta ataacttggc
     4261 tggtgctaga tataaattta ttcctaaggt tgatagaatc atacccaata taatcagaca
     4321 atattgtaaa aatatattat tatcaatact tagcttttta ctttcaccat acatcaaaac
     4381 gcaccccttt gcataataaa taaaccaccc tgcataacac acgataaatt tacaaataaa
     4441 tttatcagaa gtagtttcct aaaaatatta ttcatcctca cttaaacgtt tattcattaa
     4501 tattctatca catgacataa ataatttata tgatttatat agatttattt attgtaaatt
     4561 ttggaatatt aaaacatttt attgaataga taattatatt acataaaaaa tccctataga
     4621 aatctataaa ctaatttcta tagggatttt ggttaactaa ctattatttt tattttttta
     4681 taacttttat acctgtcata tggtcattta agttttgttc ctctaaagat ttatcatcta
     4741 ctctgtttat ttctaaagct aaagtttcag acttgatata ttcattaaaa tggtcaacag
     4801 ccttagcaat ttcatcatca ccacagtatt ctattactat attatctaat acttcaaagt
     4861 tattagattt tcttaattgc tgtactttag atataaattc tctagcataa ccttcattaa
     4921 tcaactcttc agttagctta gtatctagta taacaaaaag attgttttca acagatacat
     4981 taaatccttc cttagcagtt atacctatta atactaaatc cttattaaat tcaaaagctt
     5041 caccatctaa atcaactgtt aaagtctcac ctttttcaag tttattagca gtttcatgag
     5101 catttaattt acttaatgct ccaacgaata aattcatctt agcacctaat aatggccctg
     5161 cttccttaaa gtttggtttt aaactaaagt tcatatactc atctaaatcc ttagcaaata
     5221 ttacctcttt aacattaagc tcttctttta ttaaatcaac tacatcactt attgtatctt
     5281 caaacttacc atctactaac actttttgta atggttgacg aacttttatt ctttctactt
     5341 ctcttgaagc tctacctaaa gtaactaagt ttttagctaa atccattttt tcttctaatt
     5401 tagtatctac taactcttca ttacatttag gatattcagc taaatgaaca gatacttctc
     5461 ctgttaagtt tctatacatt tcttctgaca tatatggtgc aaatggagct atagctctac
     5521 aaagaccatg taacaattca taagttgtat tgtacactgc ttttttatct tctgttaatt
     5581 cagttgccca gaatcttctt cttgaacgtc ttatatacca gttagataaa tcatcattta
     5641 taaaatcttg tatcattctt aaagttttat ttaactcaaa tatagctaaa ttttcttcta
     5701 catctttcat taaattattg aacttagata atatccatct atcaagttct ggtctatctt
     5761 tatattctac aaaaaattca gttggattta tatcatctgt gtttgcatat aaagtaaaga
     5821 agttatatac attcttcata gtacctataa atttactttg gatttctttt aatccatcca
     5881 tatcaaattt agttggagtc catggtggag aaacatatag taaataccat cttaatgcat
     5941 ctgctccata ttggtcaaat aattccattg gatttactgt atttcctctt gatttactca
     6001 ttttcttacc atctttatca agtacaaggt ctggaactaa aacactctta tatggtgctt
     6061 ttcctaatac aaatgttgat actgcaagta atgagtagaa ccaacctcta gtctggtcta
     6121 taccttctga tatatagtca gctgggaaca attggtcaaa gttttcctta ttttcaaatg
     6181 gatagtggtg ttgagcaaat ggcatagccc cactatcaaa ccaacagtct ataacctctg
     6241 ttactcttgt cattggtttt ccacattttt cacattttaa gtgtatatca tctacatatg
     6301 gtctatgaag ttcaactgtt tctggattta catcttctat agctttttca gctaattcag
     6361 ctcttgaacc aactgaatct gtatgaccac attcacatct ccatatagga agtggagttc
     6421 cccaatatct agttcttgat attgcccagt catttagatt ttctaaccag tttccaaatc
     6481 tcttctctcc tacaaaactt gggaaccatt ttacaccatt attattttct attaatttat
     6541 ctttaagttt agtcatctct atataccagc ttggttttgc atagtatact aatggagttt
     6601 gacatctcca gcagtgtgga taattatgaa ctactttttc tttgctatat aatttatttt
     6661 cagaagctag ccattttatt atttcaacat ctagcccatc ttccattaca aatctacctt
     6721 cccaaggagt agatgtaaat ttaccagttt catctactgg ttgaataact ggtaaatcat
     6781 atcttttacc tgtgttgtag tcatcttcac caaatgctgg tgctgtatga actataccag
     6841 ttccatcttc agtagttaca taatctccta ctgtaataaa aaatgctttt ttgtcaactt
     6901 ctacaaaagg cattaattgt tcatattcta aaccttctag gtctttacct ttcatttctt
     6961 ctaaaacttc ataatcttca cctagtactt tatcagctaa aggtttagat acataatata
     7021 cctcatcacc ttttttaact tttaaataat ctacatcagc atttactgtt aatgaaacat
     7081 ttgatggtaa agtccatgga gttgttgtcc atgctaagaa gtattcatca gcatcttttt
     7141 tcttaaattt agctataact gtattagttt ttacttcttt atatccttgt gcaacctcat
     7201 gactagcaag accagttcca catcttgggc aataaggaag tattttatgc ccttcatata
     7261 tatatccttc tttgttgaat ttgtttagta tccaccatac agactctata tagttattat
     7321 ctaaagttat atatggatta tctaaatcaa cttcataagc cattctctca gtcattattc
     7381 tccattgttt ttcaaatgag aaaactgact ctctacattt ttggttaaat tcagctattc
     7441 catattcttc tatttgttgc ttactagtaa gtcctaactc tttctctact tgtatttcaa
     7501 ctggtaatcc atgtgtatcc caaccagctt ttctctttac ttgataacca gacattgttt
     7561 tatatctaca aactgaatct ttaagcgttc tagataaaac atggtgtact ccaggatttc
     7621 cattagctgt tggaggtcct tcatagaata cgaaacttgg gtcatctttc cctttttcta
     7681 aagttttttc aagtatattt atgtctttcc aataatcaaa aacttgtgct tctgcttgtt
     7741 ttacagaact gtctaccaat ggcttaaatt tcgccatgta aatcaccctt tcctactaaa
     7801 tattataatt ccaaaatttt ttattctttc acataaattt aaatactaaa aaactcgccc
     7861 cctttaaacc aaagggacga gttataaatc gcgttaccac cctaattcta tatgtaaact
     7921 aaatactagc tacatataac actctaatag tataacggta ctaaccggca cagcttagta
     7981 gaatttaatc gttcagcctg cagctcaaga gtgattttta tctttataaa ttcattggtt
     8041 ctcagcatac ccaattctct gtaaaattat ctaaagataa atgtctctat caacgctttt
     8101 aaaaatttat ctgtgtttat atgaaattct ataacaaata atttgaatag tcaatatatt
     8161 tttgaaaaat ataataattt ctatttttct tacattttta tcctattttt aatctttgtt
     8221 atagatttct caaactcaca caattattct acataaaaaa taaacaaatt acaagcttat
     8281 tctaaagttg tagcagcttc atcattacta taattataca ctgttgttcc ttcaaatgcg
     8341 tttacacatt tttcatctac atcataaaaa atctcatcaa cacttcttat ttcatcttct
     8401 aacagtgatt taaatttatt tctaaatatc ttaaattctt tgactaaagc atcatactct
     8461 tttcttatct ctataacacg gttattagct tgttctataa tttgtctagc ttttaaatca
     8521 gcttcttcaa caattatctt agccttttta ttagccgcac tacaagtgtc ttctgctgca
     8581 ctttgagcag ttattaaagt ggcttttagt gtttcttcaa tattttcata cttgtttact
     8641 tggtcttgat ataaccctaa tttttcttta agagctgtat tttccctaca tagagattca
     8701 taatcttcct tgatgatatc taaaaattca tcaacctctt catctctata ccctcttaaa
     8761 ccttttttaa actctttatt ttctatctca attggagtta gcattacatt tcctcccagc
     8821 tataatttaa attagtcctt atacaattaa tcttgcttga acttttattt ttcctttttt
     8881 agtaatatct cctatttgtg ttaccttagc tcttcctttg cctctaatag aaatcaaatc
     8941 atcatttttt actattttag aagttgaagt tatcttttca taattgacat ggactttttc
     9001 tccatttatg tattttgcac tatcttgtct agatatattg taaataccac ttatcacaca
     9061 atctaaccta tctgaagata ctgtaaatga tacttcttta tattttgaag aactattaac
     9121 aatattttct ctggaaattt cctttacaat tacattattt cttgaaacct tttctaagtt
     9181 tacaattata aaatcgcaaa tatcactact tacaactact tgacaaaagc tatcatgtat
     9241 aattatatct ccaatttttt ctctttttat acctagaccc agtattgacc ctaagtaatc
     9301 tttatgagat atatttttaa atttgaaatt cccttctatt tgtaaaaatc taagagtatc
     9361 ttctatatct tcatattcca tgtaaaatgg ataaataaat acagtgcttc tttcagctcg
     9421 ttcataacct ccatccacac tgtattttat ttcatttgta gaatttaata ttgctacagc
     9481 atttttcact tcatatggat taagaaactc cgtagactta acatcatagt tttttataca
     9541 actattagcc ttgtctatca ctttaaacat tttatttttc aagtctatat cctttatgtg
     9601 atttgttagt tttaatttat ccatcctatc accttagaat ataaccaata ctaattgtct
     9661 taggaacatc aataatacta ttgcaattaa aggagaaaaa tctataggtc catttgtata
     9721 tttatacatt atagacctta ttggcccttc tattggctct gtaagatttt ccaaaatatc
     9781 aaaaatttta ctttgtcttc catttggaaa ccaagtcatc aagcttttta taactatcat
     9841 ccatgctatt atatctaaaa gataataaag tgcaattctt attgttccca tatattaacc
     9901 gcctttttac tatttatttt gccaagggaa gaatgcttta ctttctaatt cttttttcat
     9961 atttgcatct atatccacat tattaggagc tagtataaat attgatttag aaaccttttg
    10021 tatacttcca tctaagacat aaactgcccc attcataaag ttaaatatag atttacgaac
    10081 ttcagaatta gtcaatcctt ccaaattcac tataactgcc cttctttgtt ttagatggtc
    10141 tgctatagtt gcagcttcat cataaacctt aggttcaact attacaactt tcatttggct
    10201 agttgtatgt aaatttacaa ttttatttgc ttttgcagta ctaaagccac tgttcatttc
    10261 ttcttcttga actatatcgt ccatgccact ttcatattca tcttcaatgt aatcttcttc
    10321 ttcatcaact atccaattct taaatttaga tattattcca tttgacatta ttaacttcct
    10381 ccttaaaatt tattcaaatt tgagtaatta ttttttattg tagtttcttt ctccaaagat
    10441 tgatgttcca actctaacaa tggtagcccc ttcctctata gcaatttcat aatcgtgact
    10501 cattcccatt gacaaaacat ccatccctac attagggatg tttagttctt ttatttgttt
    10561 tgataaatct ttaagacctt taaatacacc tcttatctca tcttcatctt ctataaaagg
    10621 tgccattgtc attagaccct ttatttttat atttttatat ttttctgaca cttctttgac
    10681 aaagtctaca acttcttctc tatcaagtcc atgcttagac tcttctttag atatattcac
    10741 ttgtactaag caatctattt ctctgtttat ttgttctgca cgtttttgaa tttcttcgca
    10801 taatgcaatt ctatctaaag agtgtatcat acaaacttta tcgattatgt acttaacttt
    10861 attggtttgc aaagttccta tcaggtgcca gtttaatttg tcacctataa tatcatactt
    10921 tctagctaat tcttggggtt tattttcacc aacagaagtg attccttccc tcattgcctc
    10981 attaactaag tctacatcaa cagtctttgt gactgccaaa agggtaatct ctttgctgtc
    11041 tctatctact ttttttgcag ctttatcaat tttgtttctt atctcagaca aattatcttt
    11101 aatagaaccc attattatca cctactttat ccttatattt gtgtttatac tgttaggtct
    11161 taaaattatt ttgtcatata gattaacact tgtaactcca tttatgtcat ttttataata
    11221 atccacaaat acagaagatt cattctcata agttattcct tctaatatga caaattttac
    11281 actttgtgat tgttcatcaa ttacatatac accacgttta ttgtcttttg tttttataga
    11341 agttttaggt atttttaaac attccatctg attatatatt atatcaaatt cttcgacact
    11401 tgtatcctga tttgcaacaa tttggttact tatttttaat tttactatta ttttattatt
    11461 attttcatat attttgtaaa cttttgcatc taatttttgc tcatgatatt caattattac
    11521 attttgattt ttttcaaagt tattagaatc ttctttactg atgtatgtag atagataatt
    11581 actctggtca ttggcatctt tttctatact tccttttaca tgaatctttg catcaacttt
    11641 ttcatttttt aacaccaacg tatttacatt tttgctaatt atcattacta atatattaaa
    11701 tactaaatat ataagaatac caagtatcac taatctggta tattttacct ttttcaaaga
    11761 ctctccccct ataaaattac tttcattata agtacttctt ctaaaaaatg tttattcctt
    11821 ttttataagc taatatttta cgttctaata attatttttt ccatattaaa tttatttttt
    11881 atcaatcttc ctatacaaga ctgcattatt tatatataat tgtattataa agtataagtt
    11941 taatttacat tttatacgtt aagcttactt tatatgtttt tatagttatt tttattttta
    12001 gatacaatat aaaaccaatt aagatttaaa ataacttttg ttatgttgac atttatttag
    12061 gtaatcattt acgaatcagg ataacgttat cataacaaaa gtttatgtcc taaaaaaaat
    12121 acaaggagga ttttcttatg tcagaaattt tattaggaac aggtttactt ttattggttc
    12181 tatgttgttt cacaattttt agttacaagg ccccatatgg tatgaaggct atgggagctc
    12241 tagcaaacgc tgcatgtgct actttcttag tagaggcatt caacctttct ttacttggtg
    12301 atgttttagg tatggaattt tttagaagtg ttggagctgc taatggtagt cttggtggag
    12361 ttgctgctgc tatacttgtg cctttagcct taggtgtatc tccagtttat gcagttttaa
    12421 taggattatc ttgtttagat ttttctatat tatctggatt cgttgctgga tactttgttt
    12481 ctttcttaat taagtttata gagaaaaaag tgccacctgg agtggattta gtggttgtaa
    12541 ttcttctatg tgcaccacta tctcgtctta tagcatcaaa tgtagaccca ttagttaata
    12601 gtacactact acaaattgga caagttttga tagaagctac caacattagc cctataataa
    12661 tgggaattat actaggaggt ctaataacag tagttggaac gtctccactt agttcaatgg
    12721 ctcttacatc tataattggt cttactggtc ttcctatggc aataggagca ttaggtgctt
    12781 ttggttcagc ttttatgaac tcaacactat ttgcaaaatt gaaattaggt tctaaaaaag
    12841 atattatatc tgttgctatt gaaccattaa cccaatcaga cataatttca gctaatccaa
    12901 tacctatata ttctactaat ttctttggag gagccttttc tggtatcatt gtagccttga
    12961 tgggtcttgt aaacatgaca ccaggtacag ctacacctgt ggctggtttt gctataatgt
    13021 ttgcttataa tccagttcaa caagttctta taacagctgg tttatgtgct ttattcaatg
    13081 taattgcagg atttttaggt tcaaaaatat tcagtacctt taaagttctt actgctggtg
    13141 aaatacgtgg tactgatgta gaagaagaaa ttgcttaaaa aacaataaga tttataaatt
    13201 tatatctcaa gtagtagagt tttatattgt aggcaatttt attaattgca aacgttttat
    13261 tttttattta tgtttatgat tattatgtgt gttgaatatt tagataaatt tttatttatt
    13321 atttttattt agatttttgt ttagtttttt atttggttga tatattagga ataaaaatta
    13381 gtatagttta ttactagtta gatggcataa ctttttagat acataaaaaa ttttatatta
    13441 attagatatt attaaaataa aagcgataca tgaattttta aatgtccatg tatcgcttta
    13501 tttttgccat aatcttaata actgattttt aaagtttatt atcttaacaa agttcattat
    13561 cttatataat atttaaatgc ataggcttct ttaatttaac catctaaata tttaattaaa
    13621 cattattata tgtaatataa ctaattttta aatgaatgta taggagctgg aatccttcct
    13681 cctctttgag taaataattc agatgaattt gtatttatag gcattactgg tgctcgtcct
    13741 aaaagtcctc caaattctac catatcacct acatcacatc ctggggctgg tataattcta
    13801 acggctgtag tcttattatt aatcatacct attgctgcct catcagctat catggctcca
    13861 agcgtacctg ctgtagtatt tcctggcaca gctatcatat caagacctac agaacaaata
    13921 gcagtcattg cttcaagctt atctatactt aaagcacctt taataacagc atcaatcata
    13981 cctgcatctt cagatactgg tataaacgct ccactaagtc ctccaacatg tgaacatgcc
    14041 attactccac cttttttaac agcatcattt aaaagtgcta atgctgctgt agtcccatga
    14101 gtaccaacaa cttcaagacc catttcctct aatatatttg ctaccgaatc accaatagca
    14161 ggtgtaggag ctagtgataa gtctattatc ccaaatggaa catccaatct actagaagct
    14221 tcctttgcca caagttgtcc aactcttgta attttaaatg cagtcttttt aactgtttct
    14281 gaaacaacat caaaaggctc tccctttact ttctctaaag ctctcttaac aacaccaggg
    14341 ccacttacac caacatttat aattctatca gcctcaccaa caccatggaa agctcctgcc
    14401 ataaatggat tatcttctac tgcattacaa aatactacta attttgcaca accaaaacct
    14461 tttgcatctt ttgttaattc tgcagtttct tttatgattt ctcccatttc tttcactgca
    14521 tccatattta taccacatct tgtagaacct acatttactg aagcacatac tttattagta
    14581 cttgctagag cttttggaat tgacttaatt agtatcttat caccttttgt atatcccttt
    14641 tgaactaatg ctgagaatcc accaataaaa tctataccta aatcctctgc agccttatcc
    14701 aatgtctgtg caaactttac atagtcattt tcatctgttg cacctgcaat aatggaaatt
    14761 ggagttactg ataccctttt atttacaatt gggataccaa attcacgctc tatatctttt
    14821 ccaaccttca ccaaatcttt agcagaactc ataatcttat tgtatatttt ttctctagct
    14881 ttatctccat cagggtctat acaatctaat aaagaaatcc ccatagttat agttcttata
    14941 tccaactgtt cttcatctat catctttatt gtttcaagta tatttcctac attcactatt
    15001 atcacctccc cttactgtct actacagatt atgcattgaa ttaaatattt cttctcgctg
    15061 aatttttaca tccactgata atttcttacc tctttcaacc aagtcctcaa ccgttttctc
    15121 aaaagaattt aatgatttct ccaaatcaac tatcataatc atagtaaaat aatcctgtaa
    15181 tattttttgt gatatatcta ctatgttgat attttgcttg tataattcat cactaattcc
    15241 tgccactata cctggtttat cctttccaat tactgttaaa attgctttca taataatatc
    15301 ctcccttatt tttttataaa tcttatattt ttacaaattg aactatgtgt taacatatat
    15361 ttcatttatt tgttagtaaa ccacttttta tgtatatatt taatatattt taatacctat
    15421 acattcattt gtcaattttt tttcaatatt ctattatttt tatactattt agaaaattca
    15481 tatattatat atgttttctc aacaataaat acagttttca taataatgaa aggtattact
    15541 tagcaatctc atgattttct aaataatacc tttctaaaaa ttattttttt aattttatga
    15601 atttaattat actttttcta gtatcaggat ttacaattaa tatagaacca atcataattg
    15661 gtgttagtat acaaaataaa aaaataatat ctttttttag attatattct ttagattcta
    15721 atttataatt attatttatt attccatcat aatcacttat atttgactta tttttatttg
    15781 tatcttgatt tttgttagaa atactttccc tataaggaaa agaatgttca ttggtaccaa
    15841 atactgattt tgagcttaaa tcataattat catcattttc tgtaattgaa tttttattgt
    15901 aaaatccttt ttctttatca gaattatttt ctttattacc gtctttaggt atatcatgag
    15961 gatttttatt ttcattgcta tcagtacttg attctacatc atctttagga gtatttggtt
    16021 gtttctcatc agaattatca ttttcattat tattattttc attattatta ttttcattat
    16081 tattgttttg attgttatta ctatcattat tttcatttcc attattccca tcttgattgt
    16141 tatcatcctc atctctatcc gggtcgtctc tacctcctcc tccacggtca ccatcaatat
    16201 catcattttc ccgttcatca tctggtgtct cttggttgtc tccgcctccc attataaatc
    16261 ctggtttaaa aactatattt gaataccctt cttttaatcc tcctaccact tttactctaa
    16321 aacaacgcaa cttaaaatct tcaaaaaaaa gtatcgcttg atctctaata tcctcctgtt
    16381 cttcagatat ccaattgatt ccatcgcttg aatattcaac aaaaacctta cttgcactat
    16441 acggcatatc ataaaataaa acagctacat atccattttt tgtattttga aattccagtt
    16501 ccaaattgtc tattggtttt ttctctataa cagatacatt caaaatagga actacatcat
    16561 catttattat aattttttct ttatttaaca tttctgtttt aaactttccc gttatattaa
    16621 aatcttctcc cttttccaac ttttcataat aatcagtaaa atcccattct acatctatat
    16681 caagtcgatt accactagat gtttctaata ccatatattt gggaaatttt atattttcta
    16741 ttttatcatc tttacttact tttatttcat cactaaatac accaattccc ctatgacaac
    16801 atttcacaat attatagcca gaatcttcag ctatagaggg tataagctca ttataatcat
    16861 caattatata aactttctta ctttctgata taattgattg tgccaactta ttattatctc
    16921 ttagtaaccc actttcattg ttgctactat atgcttttat tgaagtttct tctatctcaa
    16981 catcaccttt agaataaaca cctatagcat gttctccatt cacttcaata tccttattta
    17041 gtattctaat atcattttca gtataaattc ctattgcatc ttttccattt gcctttattt
    17101 tttcaacaga ttcgtaggat gatttaatat gtaagtctcc tccttcttca acgtataagg
    17161 ctgtcccatc agtagcaatt atgttgttta taccaatact tatacttcca ccattttcta
    17221 tatgtattac tccttctttt cctccttcac ctattatatt caattcatta tcatcaataa
    17281 taaatctccc atgatttttt atcaatattt tatatttgtt ggtatctatt gtaaaattac
    17341 tgtcataagg aatataaaat ctaaactctt catctattac catatcacaa gaaagagtat
    17401 aaactatttc ttctttatta tttttatttt cttgaaccca cctttcaaaa tcttcaagac
    17461 aattttcctc ttgtccaaaa actttatgtg gaaaacttat aaaacacaaa actattatac
    17521 tcataaaaat aaatatctca tactttttca tcttacactc ctagaaattt ttagctttga
    17581 aacaatcctg tcgaaacata tacttcaccc actataattt ttccacaatt atcaattatt
    17641 gtcaatttat ataagtatct ttattaattg ttataaataa aaagctactt actaaagcaa
    17701 acagacgttt cactttaata agtagctata tttttataac aaaatagtta taatattaga
    17761 acttccttca tttacaattt tttgtaaagt cttttgcatt ttaactctta tctcttctgg
    17821 catagtatat aatttacttt gtaattgctc ttttactaag tcatgtagtg atttaccaaa
    17881 catatttgat tcccataaat ctgctggttg ctcttcaaat acttccataa gatattttat
    17941 catctcttca ccttgatttt gatttccaac tattggagaa acctctgtag atatatctgc
    18001 ttttataata tgaagtgatg gtgcattggc ttttaacttg ataccatatt gttttccttg
    18061 tttcattatt tctggttctt ccagactaag ttcttctaaa gaaggagcta ctactccata
    18121 tccttttatc ttggcatcta ttaaagcact ttctatttta tcatactcat ttttaacttt
    18181 agatagtcta gttatcagac taagcagttg ataatctcct tcaattttaa atccactttt
    18241 ttcttcaagg acattataga ataaatcttg ctttgtctgt aaatcaatat ttataacacc
    18301 ttctcctagc tctacattat caacacctgt atcttccaaa aactctaact cagaaaatcc
    18361 ttgtataata ccttctatat cccttatttt acctatatct ataatacttt gttttaatgt
    18421 tgttattatg ctacttttta tccaatgatt tctttcaaga ccttcaaccc attttggtaa
    18481 gttgattctt atttctgtaa gtggaaaatc atataataca gcttccataa cctcctctat
    18541 atcttcttct tccatttcaa ccacattcat tggaagaact ggaacttcat acttttcttc
    18601 caattcactt ctaagcatac tcgtttcctc actttttgga cttaacgtat ttaaaaccac
    18661 tgcaaatggt tttttaagat ttttcaattc ttgtataact ctctcttctg gttctacata
    18721 gctttttctg tcaattccag taactgaacc atctgtcaag acaactatcc ctattgtaga
    18781 atggtctttt ataacttttt tagttcctat ctctgctgcc ttttcaaatg tcatagcttc
    18841 ttgtgaccaa ggagtggata ctaatctttg ttttccacct tcctcatgcc ctaaagctcc
    18901 ttcaactatg tatccaacac aatctaccat tcttactttt aaagatactg tgtcttttat
    18961 ttttatttct acaccatctg ctggtacaaa ttttggttct actgtcatta tcgttttacc
    19021 tgaaccactt tggggtatct catctcttgt tctatccttt ttaaattcat tgtctatatt
    19081 aggtataacc aacttttcca taaattttct tataaaagtt gattttcctg ttcttacagg
    19141 tccaactact ccaatatata tatccccctg agtccttttg gatatatctt cgtatatgtt
    19201 attattcata ttattcctcc tatacatcta atcttattaa attatattta gagttaaggg
    19261 ttaatattct taaaatagaa aaataaagac cagcaatatt aatttaattt acatatagaa
    19321 atttgtaaat ttcaatgtaa attaaattaa tattgctggt ccaaatagta gtgtattata
    19381 ttaactgact ttagtagcat tttcatttga attattattc atctcaataa gttctttacg
    19441 tctttctttt gtcatgttca tccatataaa tgtaaacacc aatccaatta tggctactat
    19501 agtaagaact atgaagtagt acctatatcc agttacaccc ggaaatgcat ccaatatctt
    19561 ccctgctgct aaaggacaaa gtacttctgg aagatatcct aacgtagcaa ctattcctat
    19621 agcagttcct gatattgata atgaataatt accttcttca agtaacgaat agtgtaaacc
    19681 ttgaattgca tacatagata catatattgc aacacatcca atcaatagta tataaatcat
    19741 agatgatttt gttggtgtaa atattacacc taaaagacct gcaatcataa gtataaatcc
    19801 ataagatata atctttgatg aaccaacttt atctccaagt aatccagaag caactgatgc
    19861 tactggtcta caatactgag ctaatattga gattgcagat gcaaacacca cagtagctcc
    19921 aaatgcttca gttgaataag gcgtaaaata ataaaatgac atattagctg aatatgaaca
    19981 aaacattatt attgttatta tccatgtatg aggcattttt attactgcaa aaaaggcttc
    20041 tttatcaaat tttgctgata cctctttttc attatccttt aatttgaaaa atactaatac
    20101 tccaactatt acatttatta ccgagtataa tactataata gctgtaagac cagatttatc
    20161 acttactttt gatgaaatat acccaaatag tatcaatact agagatagat gaattgcatt
    20221 ggtaattcca cgaccacttt ccataaatcc aaaagctttt ccttgttcat tttcagatgc
    20281 taacattcta acagctttta caagagctgg ccaaaaagta agtattgttg ttattcccca
    20341 taaggcatgg attaaaaaca caacaatata tggaggataa agtgttaata caagaccaga
    20401 tattcctgta ataattaaag aacctgaaag caattttctg gctgaaatac ggtctgctaa
    20461 aaaaccacca aaaaggtatg ataccattgc aagtagacca taaatgcttc ctaagcttcc
    20521 catttgtgta tttgtaagat taaatgcttg tgcaaaagta tcataataat aattctttaa
    20581 atatggtaaa gcatatataa atgctcctgc tacaaagagt attatcaaga ctttgaaatt
    20641 tttccaaatg cttggtttat ttacttcaac tgacatactt cctccttatg taaatacctg
    20701 taaattttca tgataattaa ttattgctta ttgtattgtt tattcaatat ttcggaatat
    20761 ttattaatat attaggttaa tctatcaata atttatggtg agttatgagc taaaagtaaa
    20821 ctcttttagc tcaccataat aattatattt ttatttatac taacgtccag agttattaga
    20881 tatctttttt tcatagttct tccatagcat taaacctgtt attataacaa aaagacctcc
    20941 tcctatcatt tcaatctcat agattatatt atcttttaaa gtctccataa ctggtgccgt
    21001 tgagaaaagt atgccaacta cagttactac aagtaccatt ctggcaatat tgatacaaac
    21061 tgaatcactt tttgcaattg aatatggtct ttcttcattt ggtcttgtct ttctaagtct
    21121 aatataagat gtaaaaagca agacatatgg aaataatgct gttaatgctg tcatagtaac
    21181 aagaacatta tatatattat ctactgatgg taataagttt gttcctacaa gaagtagact
    21241 cactattata gcctgaacta ttactgcatt tgatggtata ttgtatttat ttgtaacagt
    21301 caattgctta ggaaatacac cttctcttac atttccaaaa agcatcttta ctggacctgc
    21361 tatataaagt attaatgctc ctagtactga taaagttatt ccaaatgcta ctattcttat
    21421 aaaccaactt cctataccta aatcttgtgc aactttagct aatgcatcta gtatccctgt
    21481 agatgctgca attttatctg gaggaagtat cattgttatt gctactgaac ctaagatata
    21541 gattcctcct attaaaacag ccacagttaa tatagcttta ggaaagtttt tcttagcatt
    21601 atccatttcg gttatgaaat tagcagtcgt ttcagctcct gataatgcaa atattatagc
    21661 agatatagaa actaatgaat tggcatctat ttttggtatt atattttgga ttgtatatat
    21721 cgatgctgat ggagcttttt tgagaataac tacagacata aatgccatca ctataagtaa
    21781 tatagcagga attgtagaac ctaatgctcc tgtattcgta aaaaattttc caaatgccat
    21841 cccacgagta cttactaagg acaacagcca aaatattatg agtgaaagtg ctaaaataaa
    21901 catcttattg tttgataaat ctggatttcc aagcatatat gcaatattta tagctaaaaa
    21961 tgttaagaaa gaagaatacc aaaatatctt tgatgtccaa tttagccatg acaccataaa
    22021 tccccacttc tctccataag cttgtttaac ccattctcca agtccaccat cttttggata
    22081 tgttgcagcc aactctgcac atataagtga tgcaggaaca aagaatataa aagcaaacaa
    22141 taaccaaact ggtatagaac caagacccaa tccaaaacta gaggcagtag atttagctat
    22201 ccatctaatc ccaaatagtg ctgatatatt cataaatact aaatcccaaa atccaatttt
    22261 ctttttcact gtaattcctc caatttcaaa tttattcaaa ctcatacact atgtattgat
    22321 ttaaatatca agtataagtt cttcaacact tcttggataa ttagttataa ttgtagcacc
    22381 attcttacct actattgctg tatctgagtg tctaaatcct ccaactccag gtatatatat
    22441 ccctggttcc atagaaaata ccattccttc ttctaatatt aattcattgt caaatcttaa
    22501 atatggctct tcatgttctg ataaacctaa accatgacca attctatgat ttgaatataa
    22561 ctctagacca tactttgcaa caacagctct tgctgcttca tccaccattc ttgctggtat
    22621 accagctttt attgtatcta atccagcttg ttgagcctct actgcaatct taaacacttc
    22681 tttttgtctt tctgttggtt tgcctataat aaaagttctt tcattttctg ctctatagtt
    22741 ttcataccaa acctgtctac tatgtattac tatatctcct ctttgaagta ttctagtatt
    22801 agaatctaaa tgaggttgtg ctgttctatc aatccctgaa caagtccagt ttgcaaatcc
    22861 tatatatgta tcaggataat tttcagaaac atattttagt aaagcattat cccctgcaac
    22921 gtcaaactcc aattcactca tcccaactct aacattttct aaagaccctt ttattcctat
    22981 atcagatagg taacctgcta tcttcaaaaa ttctatttct tttgcatctt ttacatatct
    23041 catttcaaat attttacttc caacatcttt gatagcaaat gacttatcag ttatatactc
    23101 tgaaaagctt gcagatacta tatctttttc tattccaaca gttgttcctt tagggtattt
    23161 atttaaaatt atatctaagt aatgtttatg tgatattcca tgttctttca tttctggtat
    23221 ttcataatac acatatacat tatcaacttt tgcaacttct ttagcatgta attcttctaa
    23281 tccaggtact attaattcta ttttatcttg agttactact gttacaattg gtctagaata
    23341 agttattgct ttaaatccac taatataaaa ttgattgtct ggttcaaata ttaaagcaat
    23401 ttcaatgctt ttttcattca tgtactttct caatctttct agtctcatat tatttgacat
    23461 aatgcacctc tttcttaata ttcagataat ttattcaata caacaaggat gaatatatta
    23521 ttttaaaatt cttaatattt ttagtacttg tcgtatttgt atgtaaatta attttatttg
    23581 tgattatttt atcataatca tgattatttt gtcaatcatg attttatttt aaaaaatttt
    23641 ttatattcta gtgattttaa attatctaaa atataacgag tatacattgt attttgtaat
    23701 attattcttg atttatggta agtttaatga taaaatcacg attatataaa ctatattgat
    23761 tatttgtagg tgaataaaat gacaaataaa gaattacaaa agaaaaggat attgatgtat
    23821 ttcatagaag ctgctcaaaa tattatggaa aacgaaggta tagaaaatat tacacttcgt
    23881 aaggttgcag atatggctgg ctataatagt tcaactctat ataaatactt taaaaattta
    23941 gaccatttaa tatcatttgc atctataaaa tattttaaag attataactt gaatatttca
    24001 agatgtatag aaaatgtcaa tgatgaatat aagaaatata taattatgtg gaaattattt
    24061 tgtaagcatt cttttgataa tgctcaagct ttttaccagg tattttttaa cttgtctagt
    24121 gatgaactta gctatataac aaaacaatat tatgatatgt ttccagaaga tttaggtatt
    24181 catgatagtg acatatcttt aatgattaca ggagaaagta taaaggaaag aaataagata
    24241 ctattaaaca accttgtaga tactggatat ataccagcta aagatttaga cataatcaat
    24301 gatataatta tttcttttta tcaaaatcta ttattcttaa aaaagaacac aggaaaaaat
    24361 tctgatgatg atgaattaac tatgaaaatg atttcttcta ttgaatacat aataaaacat
    24421 gctgaataaa attaaaatat ggctgtctca aaatagagat aagccttttt tattgttgaa
    24481 ataataaaaa tatatttaat ttcatataaa aaagagtgcc tcgtaaacta aaaagttcat
    24541 tttgagacac ccctaaaata aatttattat ttattataca atagtttata tatcatcacc
    24601 taaaactact tcttccattt catgcttttt acttctcatc attagttcta ttccaacttc
    24661 tttaggattt gaacctttct ttattacaga atatatagca ttagttatag gcatatctat
    24721 atctaatttt tcagcaacat catgtgctac ttcagtagca gttatgcctt ctacaaccat
    24781 ctttacttct tttaaagtat cttctaaact cataccttta cctatgagta taccagctct
    24841 tctatttcta ctatgcatac ttgtacaagt aactattaaa tctccaatac ctgaaagacc
    24901 tgcaaaggta gacatattag ctcccatagc aattccaagt ctgctcattt cactaatacc
    24961 tcttgtcata agggcagctt ttgcattatc accataccct aatccgtcac atatcccagc
    25021 tccaaatgct ataatatttt ttaatgctcc tccaagttct actccaacta tgtctggatt
    25081 cgtataaact ctaagtttag ggctcataaa taaatcttgt atcatctgtg ctatttttaa
    25141 atctttagaa gcaactacta cggtagtagg aatatccctt gctacttctt cagcatgaga
    25201 tggcccagat agtattacat atggattttg tggtaattct tcttcacaga cttgagataa
    25261 tctaagccca gtcccttttt caagaccttt agctacatta acaataactt gtccttcttt
    25321 tataaatggt tttatttttt ggcaagtact tcttacagcc tgtgatggaa ctgctaaaac
    25381 tatcacctta caatctttta tagttttttc tatatcagta cttataatta tattatttgg
    25441 aaataatact cctggtaaat agtctgtatt ttctttagtt ctgtttattt ttttagcttg
    25501 ttcctctttt cgagtccaca tgctcacatc attacctttt tttgcaagcc ctaaagctaa
    25561 tgcacttccc cagcttccag tacctaatac acatactttc tccatgttta acaccacctg
    25621 agtataatat atttactatt ctttcttttg accaattttt ctctctgtcc catttaataa
    25681 tcgctttata tttgctctgt gattatatat tactgatgca gttaagaaca gagctactat
    25741 taaaccagct ttatttttta ccataatcat aaatattggt gaacatccta ttcctacaac
    25801 agaacccaat gaaacatact ttgttatagc taccaccaat ataaatactg ctagacacat
    25861 tagtgttatt actggattta cagctagcat tgcccctagt gaagttgcaa cacctttgcc
    25921 tcctctaaat cctaaaactg ctggccaatt atgacctgct acaacacata taactgccag
    25981 atatccagct aaaagagtat ctattccaac caatcttgct gcaaattctg atattaaaac
    26041 agccattaca cctttaagaa catcacctaa aaatgtcatt gctcctgctc tttttccaag
    26101 tgtcctaagt acatttgtcg aacctgcatt accagaacct tgagtcctta tatcaacccc
    26161 agcaattcgc tttgcaacta tgtatgaggt agatatattt cctaaaagat atgcaactac
    26221 agcaataatt atataactaa atatctccac tgaatttccc ccctttagtt atattttaag
    26281 atatttattt tttatttttt tgtctatact caaactttat ggaagttcct tcaaatccaa
    26341 aattttctct tatcttattc tcaaggtatc tttgatatga gaagtgagtt aaatccttat
    26401 catttataaa caatgtaatt ttaggaggtc taatgtcagt ttgagtccca tagtaaattt
    26461 ttaatcttct acctttgtca gatggtggtt gatttaacat tacagcttct ccaattactt
    26521 cattcagtgc tgaagttgat attctcttag aatgttcatt tgacacatat tcaactgtat
    26581 ctagtatctt gttcattctt tgattagttt ttgcagaaac aaatacgatt ggagcataca
    26641 tcataaatgg aaatttttct tttatatctt ttgtgtaatt actcatagtc ttattatctt
    26701 tctctattaa atcccattta ttgattacaa atatgcatcc ttttccttca tcatgtgcta
    26761 tacctgcaac ctttgtatct tgttctgtta caccttctaa ggcatctatt actatcaata
    26821 ctacatctgc tctatctaca gcactcatag ctcttattac actgtatttt tctattgtct
    26881 catatatttt actttttctt ctaagccctg ctgtatctat tagaagaaat ttctgtccat
    26941 ttttttcaaa ataagtatca attgcgtctc tagtagttcc tgcaattgga cttacaatta
    27001 ctctttcttc tccaagtatc ttattgagta tagaactttt tcctgcattt ggtttacctg
    27061 ttatagctac tcttattata tcttcttcat actctgtatc taatcctgct ggaaaatttt
    27121 caactatctc atctaataaa tctccaaatc ccatactatt ggctcctgaa actgcaaaag
    27181 gcgttccaaa tcccaactca taaaaatcgt atatattatc aaattgactt tgactatcaa
    27241 tcttattaac cactaagatt actggttttt ttgtctttct aagcaattgt gcaatttctt
    27301 tatcagcagc tgttattcca gctttaccat caactataaa aagtattaca tgtgacatgt
    27361 ccattgcaag catggcttga tttctcattt gagataatat tatatcttcg ctatcaggtt
    27421 caattcctcc tgtatccact aatgtaaaat atttatctaa ccattctact tcagcaaata
    27481 ttctatctct tgtaactcca ggtgtatttt caactattga tatccttttt cctgcaaatt
    27541 tattaaatat tgtagactta ccaacgtttg gtctcccaac tacagctaca actggcctac
    27601 ttatactcat tttatttctc cttccatctt ttgttttcta ttttaaaatt ttatccacaa
    27661 aatcttttcc ttcattcaaa caagggataa tttttattct cataagttct tctaattgat
    27721 ttaattcaat attatctaag aaaatttctt catctgcttt tagcatactt cttgttatat
    27781 aaagggcttc acctaattct ttatcttcaa gttgttcttt taaatcctta gctgttaaaa
    27841 gacctgatac agttattgtc tctccaaaaa acttgttttt gatttcatat acatttactt
    27901 gaagattttt aaacttgtcc atcatagcat ctgccatact ctgtataaat tcatatgcag
    27961 aatgaccagt agctattgat actttcttct ctctttttag aatattctca ggcaatatat
    28021 ttagatactc ttcaatctct gtttttaact tacgtatcat acctacacca tcttcaaact
    28081 gtaaaaaacc ttcatattct tcatatccag gcaattcact attagatagt atataaaatt
    28141 catctgataa aaatgcaaat cgagtaccta atttttcaag atatttctgt tgtatttgat
    28201 gtatttgctc tatagtttta cttgcgctct tatcattaaa tatatttaat tctactagat
    28261 tttctctatg ctttgtaatc ccaactggaa ctatcgcaac actgtttaca tatggataaa
    28321 gttgagctag ttcttttatt gttctatcca attcctttcc atcatttata cctggacata
    28381 aaacaatttg acagttcatt tctatatgag catctgccaa tctcttcatt ataccatata
    28441 attttcctgc aaatttattg cttatcattt tttgtcttaa ctctggatta gttgtatgca
    28501 cagatatatt tataggactt attctatatt ttataatatt atttatatct tcttcactca
    28561 tatttgtaag tgtgacaaaa tttccttgta aaaaagataa tctcgaatca tcatctttaa
    28621 aataaagagt ttctctcata ccttctggca attggtctat aaaacaaaac atacacttat
    28681 ttctacagct tttagcttgg tcaattatcg gatttgtaaa ttcaacaccc aaatcttcat
    28741 catagtcttt ttctatctcg tatatgtaga cttcgccatc ttttttttgt atttctacat
    28801 ctaaatattc atcacttagt aagaatctat actctattat atcatgtatt ggctgttcat
    28861 tcacagatat aagtaaatct cctacctcta tacccatttc ttctgcaata ctatctttat
    28921 aaaccttact tataatatta tttacttttt ttacatttac ttccaatatt ttcaccactc
    28981 ttttgtattc atcaatgctt attataatgt attttaatat gaaatttatt tatttattat
    29041 attttatgat agcattttta agcatttcat gtcataataa cacattttaa ttctaatagt
    29101 caatataagt aaatagctat aaaatagatt agaacaaata atattcaaat ttaagtttct
    29161 taaaaattta aatattattt gttcttaaaa aaattttata aatttaatgt tttactatga
    29221 ttaacgtccc atttgtcata tcatgaacca ttccacctac acactttgac atcaacaatg
    29281 ccttattttg taagtctttc tcatcatttg cataaaatat tggacaacct ccaccatttt
    29341 tcatattctt atcaagtgta actatagcta gggtatattc atttaaacct atatccattc
    29401 ccatcttatg cacttccttt ctttcctatt ttataatttt ttagtgataa gttttttcct
    29461 ttagagcttg aaagtattgg gcatgacttt acagcttcca ttactttttc cacattctta
    29521 tctactggta aataagcaat cattatactt tcatcctctt tatttcttct aggtaatggt
    29581 acgaatgaag gttctccata atctccatat aaacctaacc ttgaatatat attgtatact
    29641 attgcttgtc tttgaccgga gtcaaataat attcctgcat tagcataatc cttattttta
    29701 ggaattattt gtattcctac acctctatta atatatactt ctctatcgct ttttagacct
    29761 atatttgtta taccttttaa atctcccacc tttagtatag attcgtccac aaaactaatt
    29821 tcagcaggca ttacatcagc tatgtctcca atactttttc tagtaagaag tttttttaat
    29881 ataaaggcta acactaatcc acaaattgaa cttattacca aactcatccc ttcacttatt
    29941 ttcgtttctg caagtattac ataatacaat cctattgtta aaaaagatgt aactatacac
    30001 atataatttc ttacttcata ggttctagct atctcttcta tgaatgcagc tcctctcttt
    30061 acaagttgaa cttcctctaa gttttgcaat gtatttcttc tatttgctct cacttgtcta
    30121 aattgttctg ctgctagaga taaaaatgtt atggatgtat aagaacgatt tataagagct
    30181 ggaaccaata atgctcctaa tgaagaagcg acgaatgcta atataagttg agatagtaaa
    30241 acatttggtt gcgttggata ctgtttttgg tctaaattca acattataat tctagatgtt
    30301 ataccaataa taacagcaac aaaaaatact ctatccatta aatcactcct aaaatattta
    30361 atttgaaatt ttacatgtaa atctacaaag tctttctaga aaaatcactc tgctttcctt
    30421 ttgatgtttc tatgataggt gtacttttca ctacatctat catgacatct atatctttga
    30481 gtattggcat atatggtata accacagtac ttttttctaa attggtctta gatatagcaa
    30541 caatatcaac ctcatctaca tctctatcta tccccatatg aataaataga ttatgtatta
    30601 tagcttgtct ttgccctata tcatttacta ttccaaatgc acctaaatct cttggtataa
    30661 cttctatagc cagacattca tttctatact tttctcttgt actttctaga cctatgttag
    30721 ttattattac accatttacc attaaaatag gtccttcaaa acttattttt gcaggaacta
    30781 tatctgctat atctgctata ctgttccttc ttaaaaatct tctaaaaatc aaaccaacaa
    30841 tagctcctga cacaatagct aagattgtgc aataaaaaaa gctaaggcca tactttcttg
    30901 caaaaacgat ataaactatc gaagatacca gtgcagaaaa aagacttata taacttctag
    30961 attcatacgt agatgctatt tcttcaacgt aagcgtctcc ttttggtact aattcttcat
    31021 tatcgatatt ctttagtgtt attctttctt gttctgcaag accttgaaac tgttgtattg
    31081 ctactgcaaa aaatgtcaag gctgcaaact ccttatctat aagtgcaggt aatgctatag
    31141 cacctaacga agctgaaagc ccagatataa ttatctgttc taaataatct tgtggtcttg
    31201 atgggtattg tttgtctgta actcttaaaa ctaatcctct acataatatt cctacaacta
    31261 atgcaactat aaaagagtgt ctaaataaat ttgcattcaa taaactttct tccattttct
    31321 cagctccttc tcttatatag tttgtgcaaa aaacaaaaaa aattcccttc tatttaagga
    31381 aatttttttg taatattgta actataaatt cgctaccaat gccaagttca ctttttactt
    31441 ttatacatcc atttagtgat ttgactatat gctttgttat agccaatcct aaacccgttc
    31501 ctcctacatc cctacttctt gctttatcta ccctataaaa tcgttcaaat atgcgttcta
    31561 catcttcttt tggtatccct acaccattgt ctataacttt aattacgatt ttttcttcat
    31621 cttttaatac tacaacttgt acaatcccat tatcttgggt atactttata gcattatcaa
    31681 ctaggtttaa gaacacttgc tttatatagt ctttattgga tagtatactt attgatttat
    31741 catcacattt atataaaact tctatatttt tttcactagc tgaatgttga gttatttcat
    31801 atacctcttt aaatacatca tatactacaa cattttccat aagaagagta tctttatttt
    31861 ctacaaatga taacaacaat atatcatcaa tcaacctttt taatctatct gattcgcttt
    31921 ctattatttc taagaatcta tttctagtat tcacatctat attttcattt gactttagtg
    31981 tttcaacaaa cccacttata gatgtaagtg gtgttttaag ttcatgactt acatttgcaa
    32041 caaaatcact tctcatattt tcaagtttaa cttttttagt aatatcttct atatttacta
    32101 tcgaacctat tattacgttg ttagtattct gaagataaac tgggtctact tttatcttat
    32161 atacgatatc atctttagta gtaatattag cagtttcatt atctttagaa ccaataaatt
    32221 gaagtatttg ttttaatatt tcttcttctt ttattacaac attaatatta tgaccttcaa
    32281 ctaattgatt tggttcactc tttatcattt cttttgcttc atcatttatc agaagtatat
    32341 ttccatttac atctatagct aaaattccat gagatatact ctttaatata gaccttaact
    32401 gtaaatgttt atactgtact tcttctaagg ttttatccat attctttatc atatgattaa
    32461 aatttctagc taattcgcca agttctcctt tggctgatat gttaagccta gcatgatatt
    32521 cattattact tacttttttt gagacgtata taaactcttt taaatatttc cttaatgtta
    32581 ttgtgtatct tacagctaat ataacaatgg ctatggacaa cataacaata aaaaaatata
    32641 tactatttaa atcttccaca agataacact cctaaaatat aaccttttta atctattttg
    32701 tatcctacac ctcttatagt ctctatatat ttttccgatt tatcttcatc ttctattttt
    32761 tttcttaaat atcttatatg aacatctact gttctagttt ctccatagta ttcatacccc
    32821 catatcttat ccaaaagata atttcttgaa agtacttttc ctttattttg aattaataat
    32881 tttaacaatt caaattcctt aagagttaac tctatctttt cagagccttt ggtaacttca
    32941 tgttttgata aatctagttt taaattacct gtagtaagta tattgttttc ttcacctaag
    33001 ctagtcaaat tatatctcct taaaacagaa ctaatcctag ctaacaactc ttttatacta
    33061 aatggcttag ttatatagtc atcagcacct atttcaagcc cttcaacctt gtcattttcc
    33121 atatttttag cagtaagcat tataacagga atatttttta agtccttatc acttcttatt
    33181 tttctcaaaa cttctattcc acttatattt ggtaacatcc aatctaaaag tattaaatct
    33241 ggtttaattt ccttagcctt tataaatccg tcaaatccat catatgaata actaacttcg
    33301 taatttgaca cctccaaatt gaattttaat agctcaacta tatgcatttc atcgtctata
    33361 acaagcactt tagtattcat taaaaagctc ctctctattt tatctgtagt accgttccta
    33421 ttcgttctga tgccttatta tgttttctat aagagtggaa tttatctgct ctacaactag
    33481 tgcataaatt taaatttatt atattttctt ttttaactcc actacatcta agcatatact
    33541 cattaatttt ccataaatct aaatagtaac ttccctcttt tattatatag aacttttcat
    33601 cattatttgt aagaattgtg ttaaattttt ctactaaatc ttcagacact tcataacaac
    33661 atggtccaat tgatggccca attatacata ctaaatcttc aggaactgtt ttatagcatt
    33721 cagacattaa ttttattgtt ttatgaccta tattactaaa agtccctctc caacctgcat
    33781 gagccagtcc tatggcttta tttttagcat ctataattga tataggtaca cagtccgctg
    33841 taaacagtaa aagtggtact ttttccaagt ttgttataag tgcatctcca tctcttcttt
    33901 tccctatagt atctttattg actatattaa ctatatctga atgtatttga gaattactag
    33961 ttagattttc aaatgtcaaa tcacattctt tacatacttt taacatatct tctctatttt
    34021 ttgcatctat attcttacat gttataacta gatttacaga atttaatttt tcatcaagtt
    34081 cttcacaatt accacttaaa ttaacatact ctttaatatc ttgtacattt tttatattta
    34141 tataatcttt catcatttgt ctcctttttc tatcagtata cttttaagtt catttacaaa
    34201 agtatttata tctttaaatt gtctatacac agaagcaaat cttacatagg caacctcatc
    34261 taactctttc aatttatcca ttaccatctc accgattttt ttggtttcaa tttcttctat
    34321 aaaacattta tttatttcat tttcaatatg tacaacaata ctttcaatct cgtcaataga
    34381 aacaggtctt ttttcacatg cttttagcaa tccatatttt atcttttctc tatcaaaata
    34441 ttctctacta ttatcctttt taatgaccat taaaggagtt gtttctattt tctcatacgt
    34501 agtaaacctc tttttacatg attcacactc acgtcttctt ctgatagatt ttaaatctgt
    34561 atgtctagaa tctattacct ttgattcttt ataattacaa taaggacatt gcatctatat
    34621 tcctcctttg tttaactata tctcaaactc atctaagctt aattctgaac ctatatttac
    34681 tattattgta tcacaaccta ttttaagtat atcattccaa aatataactt cttcatcccc
    34741 tcctcttgaa aaaaaacttc tacttccatc tccaaaaatg gtaaatgaga ctaccttacc
    34801 ttcgtgaata tccatgtcta tgtctattat aaaccccatc ctttttccat tttttacatt
    34861 tataatttct ttttccatta tatcagaaac tctaatcata ttgtactcac cctttcttta
    34921 aaaataaata tgtatcgaag taaaaaaaaa tgacctatat aggtcattta aattaaaata
    34981 atgtttacag tccatggatt acttatacat attttctcat attttttaaa gcattttttt
    35041 caattcttga tacttgtgct tgagatatac cgatttcatc agcaacttct atctgagttc
    35101 ttcctttgta aaaccttaaa tctaatacca atttttctct actatttaat ttcttaatag
    35161 cttctttaag tgatatttct tgcaaccaat tttcatcagt atctttttta tcttgaacct
    35221 gatccataac gaatattgca tctccattgt cttgatatac tgggtcaaac agagatattg
    35281 ggtcttgtat ggcatcaagt gccattacaa ctgattctac ttctaattct aattcctttg
    35341 ctatttctga tactgtaggt tctttagaat ttgttctaat taatctttct ctaacttgta
    35401 gtgccttata tgctatatct tttaatgacc tacttactct aattgggtta ttgtctctta
    35461 gatatcttct aatttctcct ataatcatag gaacagcata agttgaaaac cttacatttt
    35521 ggcttaaatc aaaattatct atagctttta taagaccaat acaacctatt tgaaataaat
    35581 cgtctatatt ttctcctctg ttgttaaact tttttattac actcaagact aatcttaaat
    35641 tccctcttac aaactgttgt ctggcttctt catcaccatt ttttatttgt aataaaagtt
    35701 ccttcatttg cttattttta agaactggaa gttctgatgt attaacacca catatttcaa
    35761 ccttattaac ttgcatatat tttcagtcct ctctcaaaag tattcatcta ttgaaattat
    35821 ttacatttcc aattttttta tactttttaa gaagaattaa tttagcatta tacaaatttt
    35881 ttcatttctt tttgcaacct tgagattatt ttcttttcaa gtctagatat gtaggactgt
    35941 gatattccaa gcattccagc aacttctttt tgtgtttttt caataccttt atcaataaga
    36001 ccaaatctta attccataat ttgcttttct ctgtcactta atctgtcaag tgccataacc
    36061 aacaaatctc tatctatttc ttcttcaatt attttgtata tttcatcatt ttctgtaccc
    36121 aatacatcag ataataaaag ttcatttcca tctaaatcta tatttaaagg ttcatcaaat
    36181 gatacttctg ttttcttttt attatttttt cttaagtaca ttaatatttc attttctata
    36241 catctcgaag cataagttgc caattttata tttttattca gtttaaaagt attaactgct
    36301 tttatcaaac ctatagttcc tattgaaatc aaatcttcta catctatacc tgtattttca
    36361 aattttctcg atatgtagac tactaatcta agatttcttt ctattaaaat tgactttaca
    36421 ctttcatctg tttctagctt ttgtaaaagc tccatttctt cttctggttt caatggagga
    36481 ggaagtatat ttgcccctcc catatagtat attccctttg gctttattat ttttaattta
    36541 atacctaaca tagtaataaa gcttattatt ctttctttca atcgtaacat tccttgtccc
    36601 ccttaaattt tcacctaaaa tccaggcaat atatttggat tcattattgc tccatattct
    36661 ctatcattaa aatttgaaat accaagtatt atatttccta tcttttgctc atctacttct
    36721 atatagtcag cttttaagcc tattattatg cttgtcttat tacttccagc atgtttataa
    36781 ggtattattc taactctacc tgaagtttta tcatctaaac ttcctataat ttcttgtgct
    36841 ttcttcatat ctatatcttc ataattaata tttaatagtt tttctggaat aaatccttct
    36901 aacacactag acttaactat tattacatca ctttgactta ttgggtcttt taacaaatta
    36961 ccactatcta ctaaagcttt acagcaaaaa cttttttcta gtaagtttat atttatagtc
    37021 tttgtaagct ccttaatgta cttaagcatt ttcaaatctt tataaatgta ttttaataat
    37081 tcacaactca cataagtaca tgcaattaag aaagatattt ttaagtgact tattcctgta
    37141 aaataaatta taaaataagt gcttccagaa ataaaaatgt ttactagata aaaaactaac
    37201 gctacatgaa cataatcttt tttgctctta cttctaaatg aaattaaagt tattatactc
    37261 atgattatta ttttaaatgg tacagtaaat agtatattca ttgatggata caaataagca
    37321 acagaatagg cacctcctaa actagccccc aataacttat tttttttagg attatatctt
    37381 ttagtaagaa tagaagtaca acttataata atataattta ttactaggtt ttctataaca
    37441 tagtactcaa tatacaccat gtatcccccc ttaaaacctt taaacatatt ataagatagt
    37501 atgtacaaaa ttttatgtca cttttgatgg tcgaatccaa aacaaattct atttaaaaag
    37561 aacaatgata tatgtcttat actttatgtc gtcaaaagat atttttttaa taccaaacaa
    37621 atagactttt ttgtagaatc caatatatct aggtagaagt tattcgctct acttttggta
    37681 tatttacaaa aaaaagacta cataatttaa taatatatca agtaaatctt ttacttatat
    37741 aaaatcaaat tatatagtct gtaaattcaa atataaatta ttgtgctagt aatccatttg
    37801 catattttgc agattcattt agtgcttgtt ctggagttat gtctccagct atcatcattt
    37861 gaagttgttt ccataaatat tcatatatct ctactccatg aggtccaact gtcaatggtc
    37921 tatacatatg tttatctctt gtcaccattt cttcaaatct tgggtctcct tggtattttt
    37981 cacctttagc aactggagct ctaggtattt ctttgtggaa agcttccatt gattctttgc
    38041 ttagcatata ctcaagaagt ttaaatccaa gttctttatt tttacatgct gacattaatg
    38101 ttaaatcatc tactgaagca aaacttccaa ctgtcttatc ttctaatgat gttacatatc
    38161 cccagtttaa atccgggaat gatttatcaa atgtacttgt agcagcagag gatagcatta
    38221 tagtaaatgc agcttttcct ggtccaaata cactttcaat catttcatta ctatcttttg
    38281 ataaagcatc ttctggaata tactttttca tatcatttat aaagtttgca gctttcaagc
    38341 cttctgcatt attaaattta actgatttta aatcatcatt atatatatct ccaccagctt
    38401 gccataaata cggataccag ttccagttta agcttccaaa tactttagaa ccccaacctt
    38461 gtgcaagacc ccactggtcg atttttccat ctccatcagt atcttttgtt gctttttcac
    38521 agattctttt aaaatcatcc catgtttttg gaggtttttc tcctaatttt tctagtatat
    38581 ctttattgta gtacaatacg cctggattag cagcttctat aggtaaacca taaataccac
    38641 ccatcatttt tgcatcatct aagtatagat aattatctga agttcctgat ttttctaaat
    38701 aaggtgttaa atcttctacc gcacccattt ctataaattg aggaaacatc tctgcataca
    38761 tgtatcctat atctggacct tcacctgcac ttattgctgt tgcatatttt tctgcgtagt
    38821 tatcccatgg tactatctga aattcaattt cacagttatt ttctttttca aatttttcaa
    38881 atatcggctt ccatgcatct gcatcatggt ctgttaatgg tgcagaccat acaactaatt
    38941 tttctttacc ctctttgcct ttagaatctt ctttttttga acatcctgca agaattgata
    39001 ctgatagaat ccctaccata actaaagaaa gaaccttttt tagtttaatc atgcttaatc
    39061 ccctttcaga ctttaatttg tctttttcct caaatgttta catttaattc agatttaata
    39121 ttaacaaata agattactct tgtctaatca taaaaaacta tactaactcc tataactata
    39181 ttttttttct atacctatta gatttaattc atactaaaaa cttacttttt aaattttatc
    39241 aatatttaaa aattttttta agtctagcca caaactaact atctaaattg agcaaaataa
    39301 aaagaagtct attgacttct ttataagctt acatattatc ttcttcttct taggaatgta
    39361 ggtatctcca tgctctcatc atcatcttct tcaattatac ttgccttttt aggaggctct
    39421 attttttcct cagcaattac ttcttcttca atttctttta cagcttgttt tactgttgta
    39481 tttaatgaag aacgtatgct tgatttaggt ttagtatcta aatctaaggc accattttgt
    39541 aatccttcaa atcctgtcgc gattactgta atcattattt catcacctaa atcttctttt
    39601 atagaagctc caaatataac atttgcttct gggtcacagg attccataac taaagttgat
    39661 gcctcattaa cttcaaataa tcctaagtta ggaccacctg taacattaag aagtactcct
    39721 ttagctcctt gtattgatgt ttctagaagt ggagattgaa tagcttgtct tgcagcatca
    39781 attgctctag tttcaccact agcactacct atacccatat gagcaagtcc cttgtctttc
    39841 attatagtag taacatctgc aaagtccaag tttattaacc cttcaactgc tataaggtct
    39901 gaaattgatt gtataccttg ttttaataca tcatctgcaa ctgcaaaagc atctaacatt
    39961 gatgtatttt tttgtactat ctgtaaaagt ctatcatttg gtatcgttat aagagtatct
    40021 acttttgatt taagttctgc tattccacct tcagcgttct tcattcttat cttaccttca
    40081 aatgcaaaag gtttggtaac tacacctact gtaagtatcc ccatttcttt tgccaagcct
    40141 gcaaccactg gagcagctcc tgtaccagta ccaccgccca ttccggctgt tacaaagacc
    40201 atatcagctc cttgaagtaa ctttactatt tcatctttac tctcctcagc agctcttttc
    40261 cctacttctg gatttgctcc tgctccaagt cctctagtta atttttctcc tatttgaact
    40321 ttatattcag cttttgatgt atataaagct tgtttatctg tatttactga tataaattca
    40381 acgcctttta gttgagcttc taccattcta ttgacagcat tgtttccacc gccacctaca
    40441 cctattactt taatttgtgc acattcttct aattctacgt caaagtttag catcataaac
    40501 ccccttctct catgattcaa agttatcgta tgtattaatt atatcatttt cttcttttat
    40561 tttgttaacc tttaagtaac atattccaca aaaacgtaaa ttttcctttt taaatttctt
    40621 acttatattg tcaaatatta ctctttaagt ataaaataat ttacgtatat attaaatgct
    40681 ctttaatttt tacgtttttt gttcataata ttactaataa tatatcgtct aattatagat
    40741 aagttattaa acaatctcat accaaaaact aatacagcaa catagtaaag agaaactcct
    40801 aacttttctc ctatataagt caataatata gccaatatag cattacctat gaaaccagta
    40861 ataaaaatta cattatcata gttatcttca aaattggatt ttatcgctcc aaaaatagaa
    40921 tccattattg caagaatagc tactgacata tatatggaat aatccataga atatgtaaat
    40981 ggaatatagt aacctactac tacaccaaat gctaatcctc ctaaaaccca taacataaat
    41041 cttcacctct ttattttact ttgaaacaca actacattac aatccttttt attaattttt
    41101 tttacttttt ctttattttt ttgtattttt cgttttattt tgcaaataaa ttcactttat
    41161 ttccattatt ttcctgaact tttatattaa ttccatatat atccttaaga gatttagcat
    41221 atgattctgg agagtttata gctgcactca ataaatctat atctcctata gcttttatta
    41281 taaatggttg tccatatgta gtatcattta ctgtaatagt agctcctgaa cattttatct
    41341 tacttgttgc tagtactctt tctccattta tagaaattgc tcttgctcca gcctttttta
    41401 aatcatttaa aaccctcaaa atatctatat catgtataat caaatcgttt ggattctgtc
    41461 cgtcttttaa ctctctctca ctatccctca tagtaatagt tatgcctggc cctgtaacag
    41521 ttgaataacc acttaattct ttaacttctt caagctcttt ttgcataagt gtttggatag
    41581 attcatttcc attctttttt gcttctttat attcattcaa agtttttttg ttgtttttaa
    41641 gagtttcttt taaatttttt atttcttttt ttgtattttc tacctcactt tcaatacttt
    41701 cagtttgacc taatgttatg tatactctat ttgggtctag tgattttata taagttgata
    41761 tgaaaacgcc taatatgaat gctcctaata taactatttt ctttttcata ctattatttc
    41821 ccctctgctt tgtttatatc actatgacca tttatttcta tattgtcttc tttggtcact
    41881 tttactttgg cattataata tgtttctatt tcccagacaa taccatactt tatattaagt
    41941 gctgaagcca atgtatcaga atcaccaatt gcttttatta taattggttc ttttatgaca
    42001 gtgtcattca tataaagctt gtcttctttt aaatacatat atgtattaga aactattctc
    42061 tggtcgttta tagatatggc acttgcttgt gctgaattta atttatttat cattgataat
    42121 aataaatcat aattatatag tatactatca tttttatcag agtttcctaa gtcttttgta
    42181 gaatttatct caatttttat gcctgttcct tcaacatctg tatatccagc taatgtttca
    42241 tatttttcta tttctgactt aagtatgtct tgttctcctt cattgttttt gcctctataa
    42301 gtctctatat ctttcttcac actattaact tctgattgta gattctcttt ttcttttttt
    42361 aaagatttaa gttcttctgt aagttgctct cctcttttaa aagttgtcat ccctctattt
    42421 tctatattaa taattttaag ttgtaaacta acaagaacac ccaatacaat acttcctatg
    42481 attatacttt tatttataaa tctttttttc aatatatcac ttccttacaa ttgtctatcc
    42541 ttctggacta tacactgcca actttccata gttaagattt atagtccctc cattagtatt
    42601 tttattttgc aggtcaatc
//
LOCUS       DAEMGG010000021        41301 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEMGG010000021 DAEMGG010000000
VERSION     DAEMGG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.021"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            86..946
                     /locus_tag="KPK24_001102"
     CDS             86..946
                     /locus_tag="KPK24_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2298807.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            1020..2936
                     /locus_tag="KPK24_001103"
     CDS             1020..2936
                     /locus_tag="KPK24_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2298808.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGVFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            3023..4456
                     /locus_tag="KPK24_001104"
     CDS             3023..4456
                     /locus_tag="KPK24_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG2298809.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            4850..6859
                     /locus_tag="KPK24_001105"
     CDS             4850..6859
                     /locus_tag="KPK24_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2298810.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            7103..7849
                     /locus_tag="KPK24_001106"
     CDS             7103..7849
                     /locus_tag="KPK24_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG2298811.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            7890..9209
                     /locus_tag="KPK24_001107"
     CDS             7890..9209
                     /locus_tag="KPK24_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2298812.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRQTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(9305..10972)
                     /locus_tag="KPK24_001108"
     CDS             complement(9305..10972)
                     /locus_tag="KPK24_001108"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG2298813.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYYLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(11169..11891)
                     /locus_tag="KPK24_001109"
     CDS             complement(11169..11891)
                     /locus_tag="KPK24_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2298814.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            12282..13709
                     /locus_tag="KPK24_001110"
     CDS             12282..13709
                     /locus_tag="KPK24_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2298815.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGVRLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            13711..14481
                     /locus_tag="KPK24_001111"
     CDS             13711..14481
                     /locus_tag="KPK24_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG2298816.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            14744..15469
                     /locus_tag="KPK24_001112"
     CDS             14744..15469
                     /locus_tag="KPK24_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2298817.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEVAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            15599..17524
                     /gene="mngA"
                     /locus_tag="KPK24_001113"
     CDS             15599..17524
                     /gene="mngA"
                     /locus_tag="KPK24_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG2298818.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AVLLIITRKQKNQRKIH"
     gene            17668..20283
                     /gene="mngB"
                     /locus_tag="KPK24_001114"
     CDS             17668..20283
                     /gene="mngB"
                     /locus_tag="KPK24_001114"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG2298819.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            20543..21697
                     /locus_tag="KPK24_001115"
     CDS             20543..21697
                     /locus_tag="KPK24_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG2298820.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKDTIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(21820..23733)
                     /locus_tag="KPK24_001116"
     CDS             complement(21820..23733)
                     /locus_tag="KPK24_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2298821.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     DLFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            23975..24292
                     /locus_tag="KPK24_001117"
     CDS             23975..24292
                     /locus_tag="KPK24_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2298822.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            24300..25547
                     /locus_tag="KPK24_001118"
     CDS             24300..25547
                     /locus_tag="KPK24_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2298823.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFTVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            25576..25929
                     /locus_tag="KPK24_001119"
     CDS             25576..25929
                     /locus_tag="KPK24_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2298824.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            25951..27396
                     /locus_tag="KPK24_001120"
     CDS             25951..27396
                     /locus_tag="KPK24_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG2298825.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            27788..28540
                     /locus_tag="KPK24_001121"
     CDS             27788..28540
                     /locus_tag="KPK24_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2298826.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            28642..29562
                     /locus_tag="KPK24_001122"
     CDS             28642..29562
                     /locus_tag="KPK24_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG2298827.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWLEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            29565..31481
                     /locus_tag="KPK24_001123"
     CDS             29565..31481
                     /locus_tag="KPK24_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2298828.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKRKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGL
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            31501..32370
                     /gene="gatY"
                     /locus_tag="KPK24_001124"
     CDS             31501..32370
                     /gene="gatY"
                     /locus_tag="KPK24_001124"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG2298829.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            32879..34342
                     /locus_tag="KPK24_001125"
     CDS             32879..34342
                     /locus_tag="KPK24_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG2298830.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIVDGLEELERMLEV"
     gene            34346..35506
                     /locus_tag="KPK24_001126"
     CDS             34346..35506
                     /locus_tag="KPK24_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG2298831.1"
                     /translation="MEYRLNEVFEFIRNGASIKQFDTKFGYPITRIETIANSYIDTNK
                     MGYADIKELGKLEDYLLKNGDILMSHINSEKHLGKSAIYEDINKKIIHGMNLLCLRPD
                     KEVCYPKYLNYFFNSNEFRRQLPRIIKKSVNQASFSVNDLKNLKVDLPEMEAQKDISK
                     VLDKAQELIDKRKEQIEALDELVKSRFIEMFGNFKEQKFVLLKDCTNFIDYRGKTPIL
                     SENGIRIINAKSVGNGVFKYINEYISENTYNSWMKRGFPVAGDVLFVTEGHTFGNVCR
                     IPKDLKKFAMGQRIITIQGDKKILNNAFLAQYMQTKAFRVEIDKYKTGSSAQGIRSKE
                     LQKILIPVPSIELQNKFEEFVNQVDKLKVGIKTSLKELEDNFNSLMQKAFKG"
     gene            35551..35859
                     /locus_tag="KPK24_001127"
     CDS             35551..35859
                     /locus_tag="KPK24_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298832.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            35892..36272
                     /locus_tag="KPK24_001128"
     CDS             35892..36272
                     /locus_tag="KPK24_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBG2298833.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYKSKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            36262..36690
                     /locus_tag="KPK24_001129"
     CDS             36262..36690
                     /locus_tag="KPK24_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298834.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            36740..40075
                     /locus_tag="KPK24_001130"
     CDS             36740..40075
                     /locus_tag="KPK24_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG2298835.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKTETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            40456..>41301
                     /locus_tag="KPK24_001131"
     CDS             40456..>41301
                     /locus_tag="KPK24_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG2298836.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDI"
BASE COUNT        16109 a         4397 c         7519 g        13276 t
ORIGIN      
        1 ttttatgtaa taaattacat tttaaatatt ctaaataatt aaaaaagatt tattttaact
       61 taatgctaat aaagggggca tcacaatgat tattgaaaaa atactcaata ataatgtaat
      121 tattacaact gatgaaaatc ataaggaaat agtagttatg gggagaggtt tagcttataa
      181 aaaacgtaca ggagagcata tatcaaagga taaaattgat aagatattca aactttcaga
      241 cccaaatata tctgataaat ttaaagaact aatagcagat attcctataa gatatatgga
      301 attatcagat gaaataattt tatatgctaa agaaaaacta gggaaaagat taaatgatag
      361 tatttatatt tctttaacag accatatgta tactgcaata gaaagagctc gtgaaggagt
      421 aagtgtaaaa aatgtattac tttgggatat aaaaagattt tataagagtg aatttaaaat
      481 agggttagag gcattggatt atatagaaaa aaaatttgaa attagactat cagaagatga
      541 agctggattt atagcactgc atatagttaa tgcacaaatg gatcaaagta taaaaactat
      601 atatgagatt acacaaataa tacaagaaat ttcaaatatg gttaaatacc attacagaat
      661 agtatttgat gaagattcag tttattatca tagatttatt actcacttaa agtattttgc
      721 agaacgaatt gttagtaata atttacatga aaataatgaa gatgatttat taaatgtaat
      781 aaaagttaaa tataaaaatg cttataaatt catagaaaag ttggatgaat ttatacataa
      841 aaaatacaac tacgatttaa cagatgagga aaaattatac ttaactattc acgttgaaag
      901 agtggtaagt aaaagtatta agtcggatat aaatcctaat aattaggttt atatatattg
      961 ggtggtgaca ttaagttggc caaaccttca tatttcaaaa agaaaatgga ggagaaaaaa
     1021 tgagaaagta tgaacaacta tcaaaagaaa tcattgcgaa tgtaggagga aaggataata
     1081 ttagcagttt atctcactgt ataacgaggc tacgttttaa attaaaagat gagtcaatag
     1141 caaatgatga tgtgctaaaa aaaatggatg gtatagttac tgtaatgaaa agtgcaggtc
     1201 aataccaagt agttattggt aatcatgttc cagatgttta tgaagttgtg tgcgatttgg
     1261 ctggattagg aggagataca atagataatt cagactcaga tgcgccaaaa ggattactta
     1321 ataagtttat agacataata tcaggagttt ttcaaccaat attaggagtt ctgtgtgctg
     1381 ctggtatgat aaaaggattt aatgcagtat ttacagcatt gggtttgtat ggagaaactg
     1441 atggtgcata tattatgtta aatgcaattg gagatgcaat gttccagtat atgccaataa
     1501 ttcttggata tacagctgct aagaaattca aattaaaacc atttattgga atgcttatag
     1561 gtgcagtttt atgctattca ggtattcaat tagatactct agcatctaca ggtcaacctt
     1621 tatacaatct atttactgga acaatatttg aatcaccagt atatataact ttcttaggat
     1681 taccagtaat agcaatgaac tacacttcaa cagtagtacc agtaatttta atagtattat
     1741 tagcagctaa gttggagaaa gtgtttgcta aaataatacc agatgtagta aagaatttct
     1801 ttgtaccaat ggcagtttta ttagtttctt taccattggg atttatggtt atagggccta
     1861 tagctaccta tgcttctaat atagttggaa atggattttt agctttattt aacttttcac
     1921 caattttatg tggtgcatta actggtttat tgtggcaagt gcttgtaata tttggtctac
     1981 attggggatt agtaccaata gctatgtcaa accttatgac tatggggttt gacacaatat
     2041 tagtgggagt atttgtacca tcttttgcac aaacagcagt agtagcagca atgtacttta
     2101 aattaaaaga taagaagata aaagagctgt gtatacctgc agttatttca gggatatgtg
     2161 gtgtaacaga gcctgcaata tatggaataa ctttaccaag aaaaacacca ttcatatata
     2221 gttgtgtagg tggtgctgtg gcaggagctg taatggggat aatgaatgta aaaagttatg
     2281 taatgggtgg acttggaata tttggtatac ctaattatat aaatccaaca actggagata
     2341 tgaaaggtgt ttatgcaact ataatagcaa taatagtagc tattgtagta ggatttgttt
     2401 taacattatt tttttggaaa gatgatgcta tagtagaaat ttctaaagat gataatgggc
     2461 aaggcgttat agaaaaaaac aaaagagaag tagtatatag cccaataaaa ggtgaagtaa
     2521 aggctttgga gcagatacaa gatgcagcgt tttcaacagg tatattggga aaaggtgtag
     2581 caataacacc taaagaagga aaagtagtgg caccatttga tgggactgta atgactttat
     2641 ttccaacaaa acatgcaata ggaattatat ctgataatgg ttgtgaacta ttgatacata
     2701 tagggttaaa tactattcag ttggaaggta aatatttcaa atcttttgtt aatcaagggg
     2761 ataagattaa aaaaggtcag acattaataa cttttgacat agaaagtatt tcaaaagaag
     2821 gatattgctt agaaacacca gtagttgtta ctaattattc tgattatgtg gatattgtag
     2881 aaaatacaca aaagcatgta aataatagtc agaaattgat aactgtttta gcgtaaaaga
     2941 gagttctatt aattaagaat tatatatatg tatttttatt ttaaaataaa gttactataa
     3001 ataaattatt ggaggtagaa ttatggcact tagaaaagat tttttatggg gtggagcaac
     3061 agcagcaaat caatgtgaag gagcatggaa tttagatggg aaaggtcctt cttgttcaga
     3121 tatgtgtact ggaggttctc aaaaagtaag taaaagaata acgagagtta tagaaaaagg
     3181 aacattttat ccaagtcatg aagcaataga tttttatcac agatacaaag aagatatagc
     3241 tttatttgca gaaatgggat ttaaggtatt tagattttca atagcatgga ctagaatatt
     3301 tccaactgga atggagaaag aaccaaatga agcaggactt aaattttatg aaaatgtgat
     3361 agatgaatgt ttaaaatata atatagaacc attaataaca atatctcact atgaaatacc
     3421 atttgcaata acagagaaat acaatggatg ggtttcaaga gatgtaatag acttgtatat
     3481 gaattactgt gaaacaatat ttaaaagata taaaggaaaa gtaaaatatt ggctgacatt
     3541 taatgaaata aattcagcta caatgcctat gggaggctac ttatcacaag gaatattaaa
     3601 tgaagggact acagatttta taaaccaagt agatatacct caacttcgtt ttcaagggtt
     3661 acatcatcaa tttgtagcaa gtgctaaggc tgttaagtta gctcatgaaa tagattcaaa
     3721 ttatcaagtt ggatgtatgc aaatatttgc aacaatgtat ccttatactt gtaatccaga
     3781 tgatgcagtt aaaactcaaa gagatagtag agtaatgaat tatttctgtg gagatgtaca
     3841 agtgcgtggc gaatatccaa cttatatgaa tagatatttt ggagaaaata atattgaaat
     3901 aaaaatggaa aaaggagatt tagaaatttt aaaagaaggt tgtgtagatt tttatacatt
     3961 tagttattat atgtctacat gtgtaagtag tgattctaaa gaagataata caagtggcaa
     4021 catattaggt ggagtgaaaa atccatatct taagtctagt gaatggggat ggcaaataga
     4081 ccctgaaggg cttagatatg cactaaatga aatttatgat agatatagaa tacctatgat
     4141 ggtagttgaa aatggtttag gagcttatga taaaaaagaa tctaatggag tgataaatga
     4201 tgactatcgt atagagtatt taaaagcaca tatagagcaa atgaaggaag ctgtagaaga
     4261 tggcgttgat ttatttggat atactccatg gggatgtata gatttagtaa gtgcatctac
     4321 tggtgagatg gccaagagat atgggtttat atacgtggat aagtatgatg atggaagtgg
     4381 agacttgtct aggattaaga aaaagagttt tgactggtat aaacaagtaa tagaatctaa
     4441 tggtgaaagg ttatagtatg tagatactgt ttgtacttag aagagtagta aatagtgttt
     4501 ataaatacaa ttttttataa tgcatttaat aggtaaaagt gatatatgat ttgtacaatt
     4561 aacctataaa aatagattaa taatataaag acttataagc tataagtgct aagtctgtaa
     4621 atactattga ggaactttag aataaaatta agaaaattat taatttggca caatttttgc
     4681 tttatagaag tttaagaaat atgatagata ttgcaactat gatatttatg gacataaaac
     4741 taataaagga atatgttagt atactaaatg tacaattttt tgtgttatta aagtgaaaat
     4801 caaatgtata ttataaaaga gtagagtata ttttaaaagg agtaatcaaa tgaaaacaat
     4861 agcagtagta actgatgggt tgtctaaatt agaatgtttc ttaaataaaa atctagaaat
     4921 gatctttaaa gataaagtaa tgataaaaaa ttattattta aataaaataa cagagaagga
     4981 attaataaaa gatgacataa tattggtaat gatagatgat agagtattaa aaataaaaaa
     5041 atatgtggaa gatgtctcaa agataataac tataaatagg agcataaagc aaaaggatat
     5101 atataagtta ttttcattgc cagaaggaat agatgtatta gtagtaaacg ataataagca
     5161 tactgtattg gaaacaatat ctaatttata caatatagga ataaatcatt tgaattttat
     5221 accatacaat cccaatgaag aatatagaaa tattaagata gctataacgc caggagagtc
     5281 aagtttagtt ccaaaatata tagaagaagt tattgattta ggccataggt atattgatag
     5341 ttcaacgttt attcaaataa tagcaaaact caaactggat gataaagaaa ttactgagag
     5401 gctaattaag tatacagatg aaataattag tttatattca ggaataaata ctaaatacaa
     5461 agaacttact ataaaagcag atgaactaaa ttccataata aatttatcaa acttaggtat
     5521 ggcaatggta tcagataagg gaaacataat tatttgtaat aatagtttaa gggatatact
     5581 agatattcaa agtaatatta tagggaaaaa tatagatgaa ttagagaatg agaatattaa
     5641 aaagattttt agtttgagta aggcacatga tgaagttata aaatttaata ataagtactt
     5701 aaacgtaaat aagtataata tagagagttt tggaagggtt actggatatt atttttgtat
     5761 tcaagaaata acttacataa gaaaattgga gcaaaaccta tcaaagaagc ttagagaaaa
     5821 gggacaagtt gctagatata cttttgatga tataaaaaca gtttcaacga agatggaaag
     5881 aactaagaaa ttaggtatga aaatagctga atctgattat acaattctta taacgggaga
     5941 aagtggaaca ggaaaagaac tcatggctca gtctattcat aatgcatcac caagacatag
     6001 tcagccattt atagcaataa actgtgcagc tatgccagaa aatttacttg aaagtgaact
     6061 ttttggatat gaagaaggtg cttttacagg agctttaaaa ggaggtaaaa aaggattatt
     6121 tgaacaggct aacaatggaa ctatattttt ggatgaaata ggagacatgc ctatatactt
     6181 acaaagcaaa ttacttagag tattacaaga gaatcaagta atgagggttg gaggagaaaa
     6241 tgtaatagat atagatgtta gagtaattgc ggctacaaat aaaaatttat tagaaatgat
     6301 aaaaggagat agatttagag cagatttata ctataggata aatgtattgc ccataaatat
     6361 accaccttta agagagcgaa aagaagatat agaagttatg ttaaagcatt ttatgaaaag
     6421 gaagagagca attagtcaag atgttcaaga cattattaat gcatatgaat ggccaggtaa
     6481 tataagagaa ttaaaaaata ctgctatgta tattaacatt atgagttctg aggatgttat
     6541 tttaataaat gatttacctc ataacttatt aaatataact gaagatttta gtaaagaaat
     6601 aagcatatta aaggatagaa catctataga gaaagtaaag ttagtaatgg agtgtttaaa
     6661 aaatgccaat aatcttaata aaaatatagg tagaaatggt attgttaatt caataaatga
     6721 attaggctac aatataacag aaggtgaggt aagaggaata cttaatatgt taaaagagct
     6781 ggatttgata atatgtgaat ctggtagaaa gggaagtgaa ttaaatagaa ggggaaaaaa
     6841 tatattaaac agtatttaat aggttgataa aaaggaaaat ctaaatgcct attttaccta
     6901 ttagttgtac ttaaaacaat ttaaaaaagt caaactaaac aggattaaaa taaaatataa
     6961 gtaaataaag ttatttatat tactcgtatt caaaaaaagt atttctaaaa taaattataa
     7021 tgttcaaata aataaaatct tggtatgcta cttgctcttt aagagaagta gcatttttta
     7081 ttgttcacaa ataagttaag gggtgaagaa attggctaaa aagaaatatc caataatagg
     7141 aatatcaggt aatttactaa ttgatgaagg aggaatgttt cctggctatg aaagggctta
     7201 tgtaaataat gattatatac aatcagtagt tatgtgtaaa gctataccat atattgtacc
     7261 gattgtatat gatgatgaga ttataaagga gcaggtatct aatatagatg cgcttatact
     7321 atctggggga caagatgtaa atccactcat atggaaagaa gaacctcata ataagttggg
     7381 ggcaatatca ccaaagagag atagttttga tatgaaatta ttaaaacatg ctttagatat
     7441 gaaaaaacca gttttaggta tatgtagagg agaacaaatt ataaatgtaa ctgaaggagg
     7501 tagtttgtat caagatttat ctttaattga aggtgcttat attaaacaca atcagcaaca
     7561 tctatctaat gtacctacac atacagtaca gataaaggag gggacaaaat tatatgaaat
     7621 actaggagaa aaagaagttt tagttaatag ttttcatcac ttagttgtca acaaagtagc
     7681 accgggatat attgtttcag ctacatctaa agacggttta attgaagcta tagaaaagga
     7741 aggaagtgag tttgtaatag ggatacaatg gcatccagaa atgatgacta gagattatga
     7801 taatatgaaa aaaatattta tggcaatagt taaagaagca tctaaataaa aaatacaaat
     7861 atattaagtt ggctttgggg ggaataacaa tggaaaaagg cattaagtta ggtttttgga
     7921 gtattgtact attaggaatc aatgcaataa tagggtcagg tatattcggg ttacctggtg
     7981 atgcttatac agatataggt ccagcaagta tattagtatt agttttttgt atgcttttag
     8041 ctgtttctat tgctttatgt tttgctgaag caggtagttg gtttgatact gatggagggc
     8101 cctatcttta tgcaaaagag gcatttggag attttgttgg atttgaagtt ggatttatga
     8161 agtggatagt tagtatgatt gcttgggcta ctatggcaaa tttctttgct gtaacactat
     8221 catctgtatg gccacaagca gcagaaccac ttattaaaaa tataataatt ggtatattag
     8281 ttgtagggct tgggattata aattttatgg gaatgaaaca atctaagcac ttaaataata
     8341 tcatgactat aggaaaatta ttaccaatag ttttatttat agctgtagga ttattcttta
     8401 taaagggttc taattttacc ccatttgtaa taatacaaaa aggtcaaagt gcgtcatcag
     8461 cttttgtagc agtatctata actttatttt atgcttttac aggatttgaa tctttagctg
     8521 ttgcagcaaa agatatggag aaccctaaga aaaatgtacc taaagcattg gtaatggtaa
     8581 tgtttgtagt atcagtaata tatatgctta tactgggaat aagtataggg gtgttgggta
     8641 atggcttagc agggtcagct actcctgttg cagatgctgc cattaagatg ttaggtccta
     8701 taggtgggta tataattaca ataggaacta tagtatctgt tggaggtata aatattgcat
     8761 catccatatt tacaccaagg tctgctgctg ctttggttga acaaggatta atgcctaagt
     8821 ctataagaca aactaataaa aatggagctc cttatatagc aattatagta tctgtcatag
     8881 gaactttact aatagcatgg tcgggttcat tcacaacatt atctcaaata agtgttgtat
     8941 ctagatttat tcaatatatt cctacatgtt tagctgtact aatattgaga aagaagtatg
     9001 ctggtaaaga tgtaaatttt agaatacctg gaggagcaat tataccaata tttgcagtta
     9061 ttataagtat attattactg ataaaagcag gtattgatga acctatgaag atagtttggg
     9121 gattaggtgg gatgataatt gtagttccta tatatttcta tatgactaaa gtatattcaa
     9181 agaaatataa tgatgtagaa gtcaaataat tattgtaatc tatagatatt ctataaacat
     9241 tactttgaat ttagtaaaat tggtataaca ccagggtgtt atacctattt ttacataaat
     9301 aactttattt ataatatatc attatacttt cgtaaggttt tatttccatt ttattttcaa
     9361 ttatatctct agtgtaattg gtttcaagta ttgtgtagtt taatttattc aaataagttt
     9421 caggtaattt atatactaca ttatcatgag tgaaattact tataacaaga agattttggt
     9481 ttttatactc tctttcataa acaaatagat ttttatgatt ttcatctaac aatctataag
     9541 taccttctgt aaaaatatca tattgtttac gatatttgat taatctttga taagtataaa
     9601 atactgaacg agaatcatta agctcatttt ctacactaat attgtcatta ttaactttta
     9661 accacggttt atgattagaa aaccccttat aaatagtatt atcccattgc ataggagtac
     9721 gggcattatc tctggatttt tgttgaagta taagcaatgc ctccttttca tcaataccag
     9781 atttaatcat atgatagtat gcattcttac tttcagcatc atcatattca tctattttag
     9841 taaagtaggc atttgtcata cctatttctt caccttgata tatgtatgta gtaccttgta
     9901 gaccaaatag tgttatggct aacatcttag cactttcttg acgaaaaaat ctatcatctc
     9961 caaatctaga taaagctcta ggttggtcgt gattagagta aaatagagca ttccatcctt
    10021 ttccttcata cattttatat tgaaagtcag attgtgcttt ttttaattta tccagagaga
    10081 acatttcatt agtccatttt gcgcctcctt tataatctgt tttcatatga tggaagctaa
    10141 aaaccataga tagttcttct cgattttctc tagtatagtt gatacactct tcaggtgtag
    10201 tagatgacat ttcacctaca gtaactatat ttttaggttt gaaaactttt tgattcatat
    10261 tatgtaaata ttcgtgtata cgaggaccat cagcgtagtg agttcttcca tctccttttg
    10321 gtcctgttat agggtcatca ggtaatccag gtgttttgga gagtaggttg actacatcta
    10381 aacgaaaacc atcaatacct ttatcagccc aaaattctag tattttgtaa cattcttcac
    10441 gaaccttttc attttcccaa tttaaatcag cttgagtttc ttcaaataaa tgtaaataat
    10501 attggttagt ggtttcatca tatttccaag cagttccact aaatctggat atccagttat
    10561 taggaacaga accatcaggt ttagcatctt tccagaaata ataatcacga tatggattgt
    10621 ctttgctttt tttagattct ttaaaccatt tatgttcagt agatgtatgg tttaggacca
    10681 tatccatcat tatttttatg tctcttttat gagcttcttt taagagtttt tcaaagtcac
    10741 tcattgtccc atattttggg tctatattat aataatcttc tatatcatat ccattatctc
    10801 tttgaggaga aacgtacata ggagtcaacc ataataaatc aacacctaaa taatacagat
    10861 aatctaattt ttctataata ccatttatat cacctatacc atcattgttg ctatctttga
    10921 aactttttgg atatatttga tagacagttg cttttttcca ccaatttttc ataattgata
    10981 cctcctgtaa tttataagtt aattataata tgtttaaaca aattgtcaag cattaaacta
    11041 atattaaagc taaaatatac aataaataat tatttgtata aacacataat tgtatataga
    11101 atagtgaggt tagtatggga taaagtgtct aaacgtaaaa aaattatgtt taattaaaca
    11161 taattttttt aatatctttg agtaacatcc atgaatacaa atgtttcagg tgtatgcttt
    11221 gattcagtat attcaaaaag ctttccatca tcagtatata cactattttt aataacacca
    11281 acacaattat aatcatttag aggtagcgtt tttaactcaa gctctgtagc tttttcaatt
    11341 ttaaatatct ttctggatga aacaattttt gtacctagac actcttctat atatttataa
    11401 atagagcctt gtgctataga aacatcaagg tctttaacta tactacatag aaaatagtta
    11461 atgtcgagaa ttttaggtat gttatcaata tatcttagtc tgtgaaggta atatacttca
    11521 ctaccaactt taaaacctgt ctttttagat aacttattat caattaagat tttgctgaag
    11581 tggagaacac tagtagcagt attaaccttt ttatcttcta caatcgattc tacacctttg
    11641 aaattgtgca tatttagaaa aaaatcatta gaacaactat tttctaacac tacaacaccc
    11701 ttgcctttta tactttgaac atatccttct gtgtttaatt gagaaattgc tctacgtaca
    11761 gtgtttctgc tacatgaaaa cctttcaata agttgaagtt cagtaggcag catcatatta
    11821 cttgtatatt taccatcaag tatttcctct ttcaatactt catatatctc tgaaaattta
    11881 tttttagcca tataccccta tccctcctta aaggtcttta gtaatattat tgaactgata
    11941 tgaacaaatt atatcaaatg tgtttagaca tgtatagtga aaatagaatc atatcaacca
    12001 taaaaaggac aaaatagaat tattttgtgg gttgtttcaa atacatggtt aaacatattt
    12061 ttaaaataaa aaaaatgaat taaaaaaata gactagatat agcaatacac ttaaaaggat
    12121 ttatatgtta aactaaatga taaaaataaa atgaataaaa aatattgaca atttgtttaa
    12181 acaaatataa tatttaaaat ataaaatgtg aataaatcgt tttcacaaaa aattaaaccg
    12241 aaatagaaag gggattggat atgaagaggt tcttaaacaa aatgatgcaa caactgtcac
    12301 ttttaggaaa gactatgcta gtgccaatag ctgtaatgcc agcagcaggt atattaggat
    12361 tattatttgg tccaaatatg ctcaatgtgc cagctatatc taatatatct aatatagtat
    12421 tttcaaatgt ggactactta tttttattag gagcagctag tgcctactca aagtctaaag
    12481 acaagacaag tgtaatggtt gcagccgtag ttgcatatat gatatttaag tcaaatttag
    12541 agttattgaa tgatacatta aatgcaggtg tatttggtgg gataattgtt ggagcttgta
    12601 tagcatggac ttacaataaa acatataaag ttaaagttcc agcattttta agcttctttg
    12661 caggagaaaa atgtgtaatt acactatcac cagtggttgc tttaatttta tcatatatat
    12721 ttagtttatt atggatatat ccacaagctg caatgaatca atttggtttt tggttgggag
    12781 gagctggtgc aataggaata ttcttgtttt tattctgtaa ccgtgcacta ataccaactg
    12841 ggttacatca agttttaaat gcttacatat tatttgaaat gggtgaatat acaacatcaa
    12901 caggagaagt tataagagga gaaattccta gatttatggc aggagaccca aatgcaggat
    12961 tcttctggac aggattttac ataattatga tgttttcaat acctgctatt tcttatgcta
    13021 tatataaaac agcacaagat aaaaagaagc aagaagtaaa aggtgcaatg accgctggag
    13081 cattgacttc ttttatggca acagtaactg aaccaataga attttcgttt ttatttgcat
    13141 ctccaaagtt atatataatt cattcttttt attctggtct aggtggagtt gtactatatt
    13201 tactgggtgt tcgcttgggt tcatttaatg gagcaagtat aattgactat gtcttaagtt
    13261 ttagttatgg agataaagcg tggttagtta tacctgtagg agttgtattc tttgtattat
    13321 attatgtaac atttaaatat ataataataa aagataatgt gcaaacacca ggacgtgaag
    13381 ttgagataga gataggtgat aaagttagtg aaaaagaaaa gaatttaaaa ctttctcatg
    13441 gtaatttttc atatatggct aaacagataa ttaaaaattg tggtggttat gaaaatatag
    13501 tgactcttaa taattgtatg acgagattac gtttagaagt gaaggatgct actattttga
    13561 atgatgataa tattaaaaaa acaggagcca aaggggtaat taaattgagt aatacatcag
    13621 ttcaaatcat cataggaacg gatgttgtta aagtaaagga tgagatggaa atgcaacttg
    13681 atgagttgag aaagcaggca gacgcataaa atgaagaaac tagtagttag ggcagatgat
    13741 ataggatatt cagaaggtgt tacactaggg ataatatctt cttataagaa cggtattgtt
    13801 acaagtgctg gaatcatggt gaatatgcct ttttcaagac aagctataga aatggtaaaa
    13861 aatgatgatg tatgttttgg tttacatgtt aatttagtaa taggagaacc ttgttgcaaa
    13921 gaacatatta agataaagaa tttgttagat gaaggtggaa aatttgtttc tagcaaaata
    13981 agaagaacgc aactactgac aggtaaagag gtttttaatt atgaagaagt atatgaagaa
    14041 gtttgtgctc aaattgaaac ttacaagttg tatatgagta aattgcctga gtatattgat
    14101 atacatggta tagaagaaga tgtatcaata tcagcaatgt gtgatgctgc aaaagaatac
    14161 ggtataccaa gttgtcccta ttatttggat tcatgtatta gcatgccaag taaatatcct
    14221 cagaatgatt tttacaagtt aaataaatct tttgttaaat tatttgaaga aaaatatata
    14281 aatttagata aagatataac attgatggtt actcatccag gatttataga ttatagtctt
    14341 ataaaatcat cttctcttat tcaagagaga gtatatgaac atgcaatgct atgtgataag
    14401 gaagttaaaa tgtggttaaa aagaaataat attgaattgg ttgattttaa agatattttc
    14461 aagtctaata attatatata aagaatagat tacctaaagt tttaattaaa aataagtatt
    14521 tgaaaaaaat gtgtattttc taaaaaaata ttgatagaga tgtatcaaaa taaaaacttg
    14581 aatcatctct atcaatatag gaagtacaca ttttttatgt ataataaaaa gagtagtatg
    14641 cattaataaa ttaaaaatat taaattataa ttatattaac gaaaatttta gctaatattt
    14701 ttgaatttta cttatgaata ataaaataga tggaggagaa actatggaat tttatgaaag
    14761 agcaaaaaaa tatgagtata aatttacaga tacagaagac atgataattg aatatatgag
    14821 aaaaaacaaa gaagaaatag taaaattatc aatacaatct ttagcagata aattttttac
    14881 tgtcccaaat accatagtta ggctatgtaa aaaaatggag tatgatggtt tctctcaact
    14941 aaaaaataat ttaaaagaag agttaaaaaa tgaggataaa gagccaaaaa atggttttga
    15001 atcagatata aaaaagacat tagagctaat agattatgaa aaaataaata aagtagcaga
    15061 tataattcaa aaatctagga gtattatagt ttatggaata ggtcaaaaca ttcctttatg
    15121 tgagatatta gtaaaagaaa ttagaggtgg gcatgataat ataacttact atagtcaaag
    15181 acatgaagta cttcatgata taggaaaatt aggtgaacaa gatatgtttt ttgcacttag
    15241 tttaagtgga gaaacgaaag aagttataga agtagcaaag acagcagtta ataagggttg
    15301 taaggtagta gctctaacac atatatgtga aaatcaattg caacaaattg cagatataag
    15361 tttatattgc tattcaacaa ttaaacaagt aaatgggtac aatatatcag ataaggttcc
    15421 aattatgatg attatgagag caataataga gcaatactgg ttaagataga gatttgtgta
    15481 taaatacaca aatctttatt ttttaagaag aaattgtgtg tataatttca aaaaaacatt
    15541 ttcataattt attttatgct atattcatag cattaaatga tttaaggagg gcgtaatttt
    15601 ggaatttaag aaaatcacaa atgaaaacct tgttttttta aaacaaaaaa taaaaacaaa
    15661 gaaagacgca ataaagttct tagcaagaaa gctatatgat gaaggcaagt taacatctta
    15721 tgaggaatat ttagaagctg taatgtacag agaaacttta tctccaacag gttttgaaag
    15781 agggcttgca attcctcatg gaaaatctaa gtcagttaaa gaagctgcat ttgcagctat
    15841 gaccttagag acaccaatag aaaattggga aagcgtagac ccaaataata aagtagagat
    15901 aatattttta atagcagtac cagaaaatga aggtggaaat acacacatac aaatactttc
    15961 tgagttagtt actagactgt caaatgaaga ttatatgaat atgttgttaa attcaaaaac
    16021 taagaaagag ttatttaata acttagatat aaaagaaaag aaagaacaaa taaaagaaga
    16081 gattaggatt caaaatgaat caaaaaaaat tatattagca ataacagcat gccctgcagg
    16141 tatagcacat acatatatgt cagcagaagc attaattaaa gctggtaaag aaatcggtgt
    16201 ggatgtgtat gtagaaaagc aaggagctaa tggtatggtg gaccctcata caccagatat
    16261 aataaaaagg gcagatgcaa taatatatgc aacagatgta gcacctaaaa atacagaaag
    16321 atttgaacat ttgccaaaca taaaaacgtc agtagcagct ccactaaaaa gagcaaaaga
    16381 aataatatac gaagctttag aagtagcaca aaaacaaggt aaaggtgatt atatagaaaa
    16441 atcatctgat atatcatatt gtgaaaaatc atcatggaaa aaagatgtaa aatcagcaat
    16501 acttacagga gtatctcata taataccact tattgtggca ggaggtatga tacttgcatt
    16561 tgcagtatta gcatctcaag catttggtct acaagaacta tacaatcaag aaggttcttg
    16621 gttatggcaa ttaagaaagc tttctggagg tatgttaggt acattgatgg ttccagtatt
    16681 atcagcatat atgtcttact cactagctga aaaaccaggt cttggaccag gatttgcagc
    16741 aggtctagca gctgatttaa taggtagtgg atttcttgga ggtatgttag gaggtttact
    16801 tgctggttat atagtaaaat ttttaaagaa gaatatacca gccaaaggca cattagctgg
    16861 attcgtaagt ttttgggtat atccagtagt tggttctttg gtagtaggtt tattgatgtt
    16921 atttgtagta ggaaagcctg tagcagcact taacaatggt cttataaatt ggttaaatgg
    16981 gatgactgga gtaaatgcaa tactactagg tgcaataata ggagctatgg tttcatttga
    17041 cttaggtgga ccaataaata aagcagccta tacatttgca ataggagcaa tagcaagtgg
    17101 aaattttata ccttatgcaa tatttgcatc agttaagatg gtttcagctt ttggagttac
    17161 aatagctact gttttaggca gagatttatt ttcagaatca gaaaaggaaa taggaaagca
    17221 aacttggata ttaggattgg ctggaataac tgaaggtgca ataccattta tgatagagga
    17281 cccaatccgt gttataccat cattggtagc tggttcagcc ataactgggt ctatagtagc
    17341 attcttcaat ataggtcttc aagtgcctgg agcggggata ttctcattgg cgcttttaga
    17401 gggaagtatg tcaggattta ttatagcatc aatatggttc tttgcggcag tacttggagc
    17461 tataatatca gcagtattat taataataac taggaaacaa aaaaatcaaa gaaagataca
    17521 ttaaaagata cgttattaga tatttaaatc aaaggtttaa ttatagtgat aaaataaata
    17581 ttaaaaaatt ttgtaaactc agtagtattt ttattggatt tttaattaga cagaaaatat
    17641 tatcagataa gttttggagg ataagcaatg aaaaaaaatg tacacatagt tcctcatatg
    17701 cattgggata gagaatggta cttctcaact gaggaatcta gaatattact tgtaaataat
    17761 atggaagaaa taatggatat gttagaaaat aatcataact atccatatta tgtgatggat
    17821 ggtcaaactg ctatattaga agattattta gcagttaagc ctgaatgtaa ggagagaata
    17881 aaaaaattag ttcaagaagg aaggctaata ataggtcctt ggtatactca aactgatgaa
    17941 atggtagttg gtggagaatc catacttaga aatttactat atggaataaa agattgtgat
    18001 gaatttggag aatatatgaa aataggatat ttaccagatt catttggtca aagtgctcaa
    18061 atgccacaaa tactaaatgg gtttgatata aaatactcaa tgttttggag aggatgttct
    18121 gaaagaaaag gaactaataa aacagagttt aattggaaga gtgatgatgg gtcttctgtt
    18181 ttggttcaaa tacttccatt aggttatgca ataggaaaat atctaccaat gaatgaagat
    18241 gagctaagaa caagaatgga taaatattta ccggttcttg ataagggtgc aactactgac
    18301 catataatac ttccaaatgg acatgaccaa atgccaattc aaaagaatat atttgaagtt
    18361 atatataaat taaaagaatg ttatccagag agaaaatttt tcttaagtag atatgaaaac
    18421 atatttaaag agctagaaaa aaatgaggat atagatacaa taaaagggga atttttagat
    18481 gggaaatata tgagggttca tagaagtata ttctcaacaa gaatggatat aaaatcagca
    18541 aatgctagaa tagaatcaaa gataaccaac atattggagc cactagcttc tattgcatat
    18601 tctctaggat ttgaatatca tcatggatta atagagttaa tttggaaaga aataatgaaa
    18661 aatcatgctc atgacagcat tggatgttgt tgttcagata aagttcacca tgaaataatg
    18721 aatagatttt tcttagctga ggaaaaagta gaccaattaa taacatttta caaaagaaga
    18781 atagttgatg caatgagttg tgaaatggct ttagataagt tagtagcatt taacttaatg
    18841 ccttacgata ggaatgaaat agtaagcgct caagttataa ctaaaatgaa agcatttgaa
    18901 atatttgata aagataataa taagttggat tttgaagtgg tacataaaga agtaatagat
    18961 gcaggattaa tagaccgtca aattgttcat tatggaaact atgattcatt tatggtatat
    19021 acaataaact ttaaagataa aatacctgca atggggtata aggcgtatat gataaaagaa
    19081 gttgaacata tgttagaaaa agcttatgaa gtatgtgaca tggtagataa tgacttttat
    19141 actattcaag ttaatgaaaa cgggacatta aagatatttg ataaaaaatt aaataaaact
    19201 tttgaaaatg tattattgat ggaaaatgga ggagatgaag gagatgagta tgatttttca
    19261 ccacttccaa atgagaaatt aatatttaac gataatatag tagcaaattc cactataaag
    19321 aaaaatagat ttaataatga gataaagata aattatagat taaaagtacc aaaagatata
    19381 caagctagaa aaaataatag agcagattca tttattgact ttgatatagt tataaatgtt
    19441 ccaaatgata aagctataat agatgtagta ttcaacataa ataaccaagc aagagattgt
    19501 agggttagaa cttatatacc aacaaatata gcttcaaagt tttctgtgtc tgataatcag
    19561 tttggacata taaagagaag tgtgtatgat gatgctatgg aagtttggca acaggaagaa
    19621 tggagtgaaa gaccagattc tatatatcca atgctaacat tcgttggatt gtctgacaaa
    19681 gagcatggag tagctgttct tacaaacagt acaagagagt ttgaaatagt aggggaaaaa
    19741 tttgacacaa tagctataac attatttaga agtgtaggat tcctaggtaa agaagaaatg
    19801 gcaagacgcc caggtcgtcc atcaggaata aagttgccta ctccagattc tcaaatgata
    19861 ggagatataa aaatagactt agcaatagtt actcatgaaa aatctacact agaagcaaat
    19921 gttgcaaata tggctaaaga gtatttaaca aaaatacaaa cttataataa aatgccatat
    19981 aatgctatga aattgaatca atcagaaata gaagtagatt attcatatag tttacttaag
    20041 gaaactaata aggaccttgt gttaagtgtt gtaaaaaagg ctgaaaaaca agaggcatta
    20101 gtagttagat tttacaattc taaagaagaa acaaaatcag ctaacatagt atttaacaaa
    20161 aatattaaaa cagctaaatg gattaaccta aatgaaaaag aattagaatc tatatcaata
    20221 gataataact ctgtaaatgt agagtgctta atgaaccaag ttaaaactat actggttaaa
    20281 tagtgctaaa aaggaattat cttaaaatag agataatcct ccttatcatt gttgaaataa
    20341 taaacatata tttgatttta tacaaaaaga gtgtctcatg aactaagaaa tttattttga
    20401 gacactccct ttttataaga taaaagaata ttaatggtat aagttgtata caaagagaga
    20461 tgtagagtat aaaataaaag ataaattaag cttataaaat tatatgttta aaataaggag
    20521 taataaaaag gagtagataa atatgaaaat attaatttct atagattctt tgaaaggaag
    20581 cttatcttca atagaggcag gaaatgcaat aaaaaagggg atattgaagg ttaaagaaga
    20641 tgcgcaagta aagatattgc cacttgctga tggtggtgaa gggacagtag atgccttagt
    20701 tcaagggatg aatggtaaaa aagatactat agaagttact ggaccaatag ctaaaaaagt
    20761 ggatgcaaca tatggattat taaaaaatac aagtacagca ataatagaaa tagctcaagc
    20821 atcaggacta acattggttc cgactgaact tagaaatcct ttatacacta caacttatgg
    20881 tgttggggaa ataataaaag aagcaataaa taaaggctat agaaatttta tagttgggat
    20941 aggtggaagt gctactaatg atgctgggat aggtatgctt caagccttag ggtttgagtt
    21001 ttatgatgaa aataacaagt tagtaggatt aggtggaaaa gttttaaatg aaataagaca
    21061 tataaaaata gaaaatagat taaaggaatt agatgaatgt aagtttaaaa tagcctgtga
    21121 tgtaaataat ccgctgtttg ggaaaaatgg agctgcatac atatacgggt ctcaaaaagg
    21181 tgcaacatca gaaataattg aggagttaga taatgggctt agaaattttt ccaaagtagt
    21241 taaaaattac ttaagtaaag atgtagctaa tgtagaagga gcaggagcag caggaggatt
    21301 aggttttgct tttttagcat ttttaaattc aaaattagaa tctggaataa aaataatatt
    21361 agaagaaatt aaattagagg aagagttaaa agatgcagat tttgtcataa ctggtgaagg
    21421 tagattggac aatcaaactg ctatgggcaa ggctccaata ggagttgcaa aactggcaaa
    21481 aaaatatggg gtaaaagtaa taggtttggc tggagccaca acagaagatg cagttaaatg
    21541 taatgaagag ggtatagatg cttatttttc tatagtaaat agagctatga caatagaaga
    21601 agcaatggat aaagcaactg ctagtgagaa tatgacagct acaacaacac aaatattcaa
    21661 tcttataact agtatacaac agtcaaaaaa tatgtagtat taaaacaagg tatattaggt
    21721 aaaaagctgt cacatattaa tttagacttt ttagtgtgac agcttatcta ttttatgtta
    21781 ataacttaag aaacatatat tggaaataaa ttaattgatt tagatatttt gaataagata
    21841 agctattcta ttcataaaac tactatagtt atctgcctta gtaagttctt taacattatt
    21901 ccactcagat aatatttcaa taaagctttc aaatatgtaa ttgaactttt ctttttcttt
    21961 ttcatttata ggtatcatag ctataatttg aactttttcc tcaccccatt taactggttt
    22021 atcgttaaca attaaacata ctccggtttt taaagcatcc attttcatgc tatgaggaat
    22081 tgctacattg ttattaaatg cagttgaaga cattttttct cttataagaa catcatctat
    22141 ataattgggt ttgacatatt tattttcgta cagaatattt cccataaatc ttatataatc
    22201 tttagcacta tctaaatata tatttttcat aaacaaagat tcactaaagt acatttctaa
    22261 atgattttgt acagtaagta tattttttct atctttaatc tgaataaatt tactttgaat
    22321 attatcataa tcctttctat ttaggtaagg atttacataa acaaaaggta tagggcatct
    22381 atcgctaatt ggtaaagtag taataaccat gtctacctta ttgtcaaaat caagattatg
    22441 agtatctgaa aaaatctgaa taatttctat ggattcatta aatctcttct caaggtctct
    22501 tactattttg aattgtaaat catagtaatt tggagtaatt aatgcacaga gaattttatc
    22561 ttctagtaat ttttgtctat caaaaaatga acctacatga agagatatat acgatatttc
    22621 atcttcatct atttctatgt tttctaaaat ttgaatctga ttacaaatat aaacagatat
    22681 atcatatatc agagggtagg aatcttttaa cttctgaggt atttgatttc tcaaaacttg
    22741 attattttta gccctactga ttaaattttt tacatgaaga gtaaatttta caacaaattc
    22801 atcatcagat aaatctataa agtataagtc atatacattt ttaattattt ttttagttaa
    22861 tttaacaaaa tgttcatcta tataatttgg caatgactta gtatccattt catgataatt
    22921 taagactgtg gttttgtttg ttaactgaaa tactaagtag tataattctg cactagttaa
    22981 agttatattg tattcagact ctaaaaaatt tgatatatct cttgaaatgc ttatatcatt
    23041 attattatct atgtagttta ctacttcgac agattctatg tagttattct tttttattct
    23101 gtctatagtt atcattaaat gaagaagtat gttatttata gcatactcat ttataaacaa
    23161 attgtatttt tttaatatat atattatatt ttctttcagt ttggtcaaat tatactcttg
    23221 atatattttt tgtacatcaa ataagcttaa aaaatcactt cccgtttctt taaatattat
    23281 agagctcatt aatttgcgct ttgcagactc atttccaatt agtttcaata aaaatttcga
    23341 ttgctttatc tttaaattat aacttcctat aaatttatta gctttgacta tatctccttc
    23401 aatagtagct ggactaacaa acaagtcttc acttaaatca aatatattaa ttccattact
    23461 attaactata agttctttta aaattaaatt taacctgtta tcaggtgata tattttctga
    23521 actatcaacc ttggtttgac aattattatt gagatttact ttatatcctt ttttagagga
    23581 taatataaat tcaccattgg aattaatttc gtttacatat tttctaattg aacgcgttga
    23641 gacattaaga taattagcca atgtagatga atctatccaa tcatgttgtg tttttatgaa
    23701 atttaaaaat agttcttttt tagatgtttt cattagattc cctcgcatag ttttaaaata
    23761 aaatctggat gatatgataa ttatataatt gaatgtacgt attataagat acattatact
    23821 atattaatat gtttaattaa gagtaactga tttcctatta ttaggaaata tatttatgtg
    23881 aaatgaactt atttggtaag tacaatataa acataataaa ataaaaagtt cattaagaaa
    23941 taaattgatg gataaaaata ttggaggtat cattatgaaa aatataagag tgcttttggc
    24001 gtgtggttca ggagcaagta caggttttat ggcaactagt atgagaaaga gtgcaaagaa
    24061 aaaaggtata gaagcagata taaaagcgag aagtgagtca gagatagata agtatttatc
    24121 agaaatagat tgtttattac taggaccaca tttagattat atgaaggaag aaattcaaca
    24181 aaaggctaat gaatataacg ttccagtagc atgtatatct aaaactgcat atggaagact
    24241 agatggggca aaggctttgg aagaagcact agaactaata tcaaacaaat aaaatattta
    24301 tggggagagt gtttatgaat aaatttatag attttttaag tgaaaaattt actcctgttg
    24361 taaataatat gactaagaat atatgggtac aatcagttca atcaactata atgaaagttt
    24421 tgccaatggt atttgtaggc tcattagtaa ctatagtttc tgtcttgaag aattacataa
    24481 gctttttacc tgatttaagt ccaataaatc aatatacatt tggactttta ggattattta
    24541 tagcattttt attgcctatg gaaataatga agaataagaa atttgaaagt atgagtgtag
    24601 tcgctggtct tgcaggtgct ggattaatgt taatgatgat tcgtccagag attacgaatg
    24661 aaggagctat atttaacttc aacaggtttg gaggagaagg aatgcttgta tcgctagttg
    24721 caggattatt ctctggatta ataatgtcct tatttggttc tttttcattt ttcggagaag
    24781 attcagcact tccggatttt gtaagcaagt ggtttgataa tatgttacct ataacagtga
    24841 taatgtttgt aggctgggga ttaatttatg gactcaactt tgacatgttt gaaataatag
    24901 ttagagtatt taaaccagta tcagatatag cacagacatt acctggattt gtactcttaa
    24961 actttttagt agtatttata tattctttag gaataagtgg gtgggtacta tctcctataa
    25021 cttattcaat tcaacttggt gcaattgcag ctaatgcagc agcagtagca tcaggaggag
    25081 tagcaactaa tatatatgtt tacgaagtta ttcaaacagg ttggataact ttaggagggt
    25141 tgggtgctac attaccatta gttataatga tgtgtttatc cagagttgga agaataaaag
    25201 caataggaag agcatgtatt gtcccttcta taatgaatat aaatgaacca gtaatatttg
    25261 gagctccagt ggcatggaac ccatttttaa tgttacctat gtggatagga acagtagtaa
    25321 tatcaataac tacttactta tcaatgagta tgggatttgt gacaattcca agtaaggtgt
    25381 ttaatttatg gtatgttcct tttggtatat ctacttttac ggtaaatcaa gatgttagag
    25441 gtcttattgt tttagcaata aatatagtat tgctatttat gatatggtat ccatttttta
    25501 aagcttatga gaaagaagag ataaaattag agagccaaga ggtttaaatt tagaaattga
    25561 aaggaaatta taaatatgac aattgaagaa catataatag atgaagaatt agtagaggtg
    25621 gcaatgcaaa taatacttcg tgcaggtgag gctagaacag aaattaaaca tgctcttaat
    25681 gatttggaaa agtttgatta tagaaatgct gatttaaaac tagctaaagc aaaagggttt
    25741 atgacagaag cacatagagc tcaaaccaag attatacaag gggaagctgg tggagagaaa
    25801 agagcacatt cgctgttatt tgcacatgct caagatactc ttatgaccat attcagcgag
    25861 ttaaatataa ctatgtcttt agtaggaata gtaaagtcta tagaatataa ggtaaataat
    25921 agtaaatgat ttaaataaat aaaataagga gtgattaaat tgggtaagtt tcctaaaggt
    25981 tttttatggg gaggagctgt agcagctaat caattagaag gtgcatggaa tgaaggtggc
    26041 aaaggttggt gtttagctga tgtacatatg tatgataaag atagagatat atctaaacct
    26101 tatgagtctg atatgactat agaaagaata aagtttgcta tgaaagataa taatggatat
    26161 tatcctaagc gtagtggaat agacttttat catacataca aagaagattt aaagcttttg
    26221 aaagagctag ggttaaagtg ttttagattt tcaataaatt gggctagaat atttccaaat
    26281 ggagatgatt tagagcctaa tgaagaggga ttaaagtttt atgataatct tataaatgaa
    26341 gtaatagata atggtatgga acctattgta acactacttc actatgagac tcctttgaat
    26401 ttagtattaa aatataaagg atggtctaat agaaaagtgg tagatttctt tgttagatat
    26461 gcagaattaa tactaaatag atataaaaat aaagttaaat attggatagt tataaatcaa
    26521 ataaatttaa tacattatga atcatttaat tcagttgcat tttgtgtaga ccaagtagat
    26581 aatgtagaag agactagata tcaagctatt catcatcaat tcatagctac agctttgact
    26641 aagaagcttg gaaaagaaat aaattcagat atgatgatag gtatgatgat ggcagattgt
    26701 acatcttatc cagaagattg tgaccctgaa aatgtagtat ttgctttaaa aagaaatagg
    26761 ctacaatatt ttttcactga tgtaagtttt aggggagagt atccagtcta tattaagaga
    26821 tttttcaaag aaaataacat aaatatagtt atggaggaag gtgatgataa aatcttaaaa
    26881 gaaaatacta tggattttct agctttatca tactattatt ctacaacagt atcagcaagt
    26941 aaaaacacca tgaatccaac tgacaatata aagaatccat atttaaaagc aaatccttgg
    27001 gggtgggcaa ttgacccaaa aggattatac aatactttat ctcaatatta tgatagatac
    27061 caaaagccaa taatgatagc tgaaaatgga tttggaatgc atgataaatt tgaagatgga
    27121 aaagttcatg atagttacag aatagattat ttaggaaaac atatagaaca aataggtgaa
    27181 gctatagctg atggaaccga aatattagct tattgtatgt ggtcaccaat agatatagta
    27241 agttgttcat ctcaagaaat ggaaaaaaga tatggattta tatatgtgga tatagataac
    27301 aatggaaatg gaagtaaaga acgttataaa aaagatagtt tttattggta tcaaaatgta
    27361 ataagaacta atggagaaga attaagagaa aagtaaatag tttttaaata taaacaaccc
    27421 cccttataaa tactgtatat attattaata tagttttata aggggggtta taattgtaaa
    27481 taaaatgatt tattaattat atgattcata gcgaaattta tatttatact tttaatcata
    27541 aacatatcag tatgaggaat atctattgta cttcatccaa attttggtag aggtacaatt
    27601 cttttttatt ttaaaatgct ataagataac ataataaact ttaagcaaaa aattattata
    27661 ttataaattt acatatatag acattgacta aatcttaatt gtatataaaa ataagtagtt
    27721 aagaggtaat atattatatt actcgataaa tttagacaaa taaaacttat ataaggggag
    27781 ataacatatg ataattgagc taaacaaaag taattctgaa aatctaacga agacggaact
    27841 tgaaataatt aaatttataa atgaaaatga atcaatatta ccagaattat ctatagtaga
    27901 gattgctcat gaaacatatt catctccagc aaccgtttca agagcaatta gaaaatgtgg
    27961 tttaaatgga ttcaatgaac ttaggtatag attaacagta aaagatgaaa atgatgatat
    28021 acaaaatatg ggggatataa taaataaatc ctttatagaa gcacagagag ttatagaaca
    28081 aatatcttta actacactaa tcaatataat aaaaactata aataaatcta gtaagatata
    28141 tattttagca agagggctta ctgaatatgt agcaaaagag tttagtctta aattgcaatt
    28201 acttgatttt aatgttgttt ttataaatga ccccaacata atgaaaataa aatctaaatc
    28261 aataaaaaaa gacgaactta taattatatt ttcattgaat ggaaatacca tagagcttgt
    28321 agaatcagca cagaatgcca atttagttgg agctaaggtt ataacttgct gttgtagtga
    28381 aagtgcaaaa cttataaagt attcggattt atatattgta ggatacaaac atgaacatat
    28441 atcaatttct gaatatgaag tatcttccag attgcccttg tatattatat ctaggataat
    28501 tatagattat atggtcagat ataataattc tgaaaattaa attgaaaagt ctatgaaaat
    28561 cgattttcta tttgtgaaaa gattttcata gactttttta ttataatgaa aacatacaca
    28621 gcacaagcaa gggggaagac aatgatatat acattaacta cgaatcctgc aatagatatg
    28681 aatatatcta ctaatgggat aaagagaaaa ttagtaaatc gtacatcaaa tgctgtatat
    28741 tctcctaatg gaaagggatt aaatgtaact tttgtactag gtcattatgg tatagaatct
    28801 aagatattag gattttttgg aggattttca ggaaaatata tagttgaaga aagcgaaaag
    28861 agaggatttg acgtattgcc tacttggtta gaagatacaa ctcgtataaa tatattttta
    28921 aatgatggaa gtgatgaatt taaatttgta aactctgggt catatgtaaa cgaaaaacaa
    28981 aaactagata tgctagataa gatacagtca ttagaagata taagttactt atcaataagt
    29041 ggaagtctac ctcctggtat tgatgataat tactatgagg atatatttaa aatttgtaaa
    29101 aataaaagta taaagacaat actcgatata agttcaccaa aattaaagga gttactacag
    29161 tataatccat atttgataaa accaaatgat gaagaaatca aagatatatt tggaattatt
    29221 gtaagagatg aagaagatat taaggatgta cttaaattac tacatttaaa aggtgctcaa
    29281 aatatattgc taactttagg agaaaaaggt tcttattttt ataatggaaa atcaatttat
    29341 tatgctagtg ctcaaccagt tacagtagtc agctctgcat gtgctggtga ttctgcatta
    29401 gctgcttttt taagtatatg gttggaaaat ccagaacata ttgaagaagc attaaaaagg
    29461 tcagcagcaa caggagcaag tgtggctgaa agtaatggta taggtaattt ggagaatgtg
    29521 gaagattata taaaaaatat aagagtcaga aaggtggtat aaaaatggga aagaaaatga
    29581 ttctaggggt aacaggttgt ccaactggaa tagctcatac ttttatggca gaagaagcat
    29641 taaaaaattc agccagggaa ttagggtgtg acataaaagt tgaaacgaat ggagctatag
    29701 gtgtagaaaa taagttaaca gcaaaagata tcgaaatggc agatgcaata atagttgctt
    29761 gtgataaaaa tgttgatatg gacagattca atggaaagcc agtaatagaa gtacctgtca
    29821 aagaaggtat acataaagct aatgaactta tacaaagatg tattgatgga aaagtagcta
    29881 ttagaaaagg aaacttatca agcagtaaaa ttgcagaaga aggaaattta tcgtttggtc
    29941 aaaagttgta taaagactta atgaatggtg tatctcacat gttaccttta gtagtagcag
    30001 gtggagtact tactgctata tcatttttat gggggattta ttcatttgac ccaaattcag
    30061 aacaatataa tcaaatagca gctacattaa aatctgttgg tggatattct atgaacttga
    30121 tggttcctgt attggcagca tttatagctc aatctatatc gggaagagct ggtatgttgg
    30181 caggacttgt tggaggaatg atatcatttg atacaggttc ggggttttta ggtggaatca
    30241 tatcaggatt tttagcaggt tacttagtta aactatttgt acatttgtta agaaaattac
    30301 cacgacaatt agaaggtctt aaatcgattt ttataattcc tattgtcagc gtaggtcttg
    30361 tgggtgtagc aatgttgtta cttggtgggc catgttcagc acttaacaat gctatgatga
    30421 actttctatc ggggttacaa aattcaagcc ctataatttt gggaatagta ataggatgta
    30481 tgtcagcatt tgatatggga ggtccagtaa ataaagcagc atatgtaact ggaactatgc
    30541 ttctaggaca gggaaattat ttatttatgg caggtgtatc tgcagcttgt ataacaccac
    30601 cattggtaat agcaatagca tctactttat ttaaaaatag atttacagaa gaagatagag
    30661 cagcaggatt aatcaattat atactaggta gtactcatat aacagagggg gcaatacctt
    30721 ttgcagcaaa aaatccatta aaagtgttgc cagtacttat gattgcatca tcaatatcat
    30781 caattttaac ttatataatg aaaatagaag taccagctcc acatggaggg tttttggttc
    30841 taggattagt caataagcca cttttatggg tagggtgtat atttgtaggt tcattagtag
    30901 gtgctgtact ttatatgatg gttacaccta aagtagtaaa tgagaaaaat actatagaaa
    30961 atacaaatgt aacaaaagat gaacataaaa atgaaagtgc agaatcggaa aacgtatcta
    31021 ataatatctc tttatatagc gaagaaacag taattttaga tgttaagaga aaaaataaaa
    31081 cagatgtaat tgatgaaatg attggaatac ttgataaatc tagtgttctt ttagataaaa
    31141 agaagtttaa agaagaaatc cttaaaagag aagaaatatc atctacaggt tttggtatgg
    31201 ggatagcgat accacatgca aaaacagatg cagtaaaggt accacgtgta gcagtaggtc
    31261 tatcaaaaga agggtttgac tttgaatcag aagatggaag cttggcacat attatattta
    31321 tgatagcagc tacagataat ggtgataatt tacacttaaa aacattatct caactatctt
    31381 caaaattaat gaatgaagaa tttttaaatg agttattaaa tagcaaaaca agtagagaaa
    31441 tagtatcaaa attaaataac gaagaaatta aatcagtata aacatatagg aggagaaatt
    31501 atggcattag tgacaactaa acaaatatta ttagatgcac aagaagggca ttatgcagta
    31561 ggtgcattta atgttgaaaa tatggagatg gttatggcag taatagaggc tgctgaagaa
    31621 cttaaatcac cagtaatatt gcaaacaaca ccttcaacaa taaagtatgc aggactagat
    31681 tattatttag cgaatgtaaa ggtagcatca gagaaggcaa gtgttccagt agcaatgcac
    31741 ttagaccatg gttcaagctt tggactagct atgcaggcac ttagagttgg atatacttct
    31801 attatgattg atggttctca tgaaagttat gaagataata tttcaatatc taaatctgtt
    31861 gtagatgcat gtatgccttc agatatacct gttgaagctg aattaggtaa agttggaggt
    31921 aaggaagacg atttagatgg tggtgatggt ggagcatata cagacccatt agaagcaaaa
    31981 gagtttgtag agagaacagg ggtatcatct ttagcagtag caataggtac ggcacatggg
    32041 ctatataagg gagagccaaa acttgattta gatagattat ctgaaattag agaggttgta
    32101 tcagttccat tagtattaca tggtgggtct ggaataccag atgaggttat taaggagtct
    32161 ataaaaagag ggatttgcaa ggttaattat gcaactgaat taaggattgc ttatagcaat
    32221 ggggttaagg atgtgcttaa ttctgaccca gaggtaattg accctaaaaa atatggtaaa
    32281 aaaggtttag agtcagttaa agattttgtt aagagtagga tggaagtttg tggatgtgta
    32341 ggaaaagcaa tagagatagt gaagtgttaa taataaaggg aaagaataag taagattaaa
    32401 ttaaggggtg tctcaaaatg aacttttagt tcatgaggca ctcttttttg tatgaaatta
    32461 aatatatttt catcattccg acaataaaaa agaagattat atctattttg agacagcatc
    32521 ttattttatt ttttgattaa gattgtaatt gagaagtttg aaataaggaa gatgatataa
    32581 ttaaataaaa aatttctttc taagtttaaa ttaatattaa aaagttataa tatggtaaga
    32641 gttatccagg tagatgactt ctatgatgga tatatgataa aataattttg tatggtatat
    32701 ataataatca taaaataaga gtattgcatt agttttttag ctaaaatatg taaataggaa
    32761 aaataattta ataattgtgt agtaaaaact tggatttaac ataagacact aactattaag
    32821 gcgtacgact aaaatatata tttaaattat tttatgaaca ctggagggaa agtcatatat
    32881 gattacagga gaattaagga gtaaagtgga caagatttgg gagacttttt ggactggtgg
    32941 attaactaat ccattagaag ttatagaaca atttacatat cttttattta ttaaaggatt
    33001 ggatgaagta gaaacaaaaa atgaagcaga agcaactctt ttgggtgttt cttttgaaaa
    33061 gatatttgat gatgaacatc aacatttaag gtggagtaag ttttctaatg aaggaaattc
    33121 agaaaaaatg tatgaaatag tacaaaatga agtgttccct ttcataaaaa aattacatgg
    33181 aaataaagaa tcagcttatg caaagtacat gggagatgca atatttaaaa ttcctacgcc
    33241 tcttatgtta tctaaaatag ttgatggtat tagtaattta gaattaagta aggatagaga
    33301 tacaaaggga gatttgtatg agtatttatt atccaaagtg gctacagcag gtactaatgg
    33361 tcaatttagg actccaagac acataataga tatgatagta agacttatta aaccaacacc
    33421 agaagatatt atagtagacc cggcagcagg ttcggcaggt tttttagtat catcacaaca
    33481 atacttaagg gataatcatt caagtctttt cttagtgcaa gggctgaagg agcattttaa
    33541 taatggtatg ttctatggat ttgatatgga tagaactatg cttagaatag gtgctatgaa
    33601 tatgatgttg catggcgttg acaatcctaa tatagagtat aaggattctt tatcggaggt
    33661 taatacagat aaagataagt ttacattggt gttagcaaat cctcctttta agggaagttt
    33721 agattatgaa gcagtgtcag cagatttatt aaaagtgact aagacaaaga aaacagagtt
    33781 attattcttg gcactttttt taagagtttt aaaaattggt ggaagatgcg catgtatagt
    33841 gccagatgga gtattgtttg gttcaacagg tggacataaa tcgataagaa aagaaatagt
    33901 tgataatcat aaattggaag ctataatatc aatgccaagt ggagtgttta agccttatgc
    33961 aggagtatct acagctataa tgatatttac taaaacagga acaggtggaa ctgataaggt
    34021 atggttctat gatatgaaag ctgatggtta ttctttggat gataagagaa atgagataaa
    34081 ggatagtgat atagaggata ttattaagag atttgataat cttgatggtg aagtagatag
    34141 aaaaagaaca gagcaaagct tttttgttga tgtggaagag ataagagaga atgggtatga
    34201 tttatctatt aataagtata aagaggtagt gtatgaggaa gtagtttatg atgctcctag
    34261 tgttattata ggtagagtta gggagttaga gaaggaaatc gtagatggat tagaggaatt
    34321 agaaagaatg cttgaggtgt agagtatgga atatagattg aatgaagttt ttgaatttat
    34381 tagaaatggg gctagtatta aacaatttga tacaaaattt ggatatccaa ttacaagaat
    34441 agaaactata gcaaactctt atattgatac taataaaatg ggatatgctg acataaaaga
    34501 gttaggaaaa ttagaagact atttattgaa aaatggagat atactaatgt cacatattaa
    34561 tagtgaaaaa catttaggga aaagtgcaat atatgaagat ataaataaaa aaattataca
    34621 tggaatgaat cttttatgtt tacgaccaga taaagaagtt tgttatccaa agtatttaaa
    34681 ttattttttt aatagtaatg aatttagaag acagttacca aggataataa aaaaatcagt
    34741 taatcaagca agtttttcag ttaatgattt aaaaaattta aaagtagatt tacctgaaat
    34801 ggaagctcaa aaagatatat caaaagtttt agataaagct caagagttaa tagataaaag
    34861 aaaagagcaa atagaagcat tggatgaatt agttaaatca agatttattg aaatgtttgg
    34921 aaattttaag gaacaaaagt ttgtattatt gaaagattgt acaaatttta ttgattatcg
    34981 tggtaaaact ccaattcttt cagagaatgg aattagaata ataaatgcaa aatctgtagg
    35041 gaatggtgta tttaaatata ttaatgaata tatatctgaa aacacctata attcttggat
    35101 gaaaagaggt tttccagtag ctggagatgt actttttgtt acagaaggtc atacatttgg
    35161 aaatgtatgt aggataccaa aggatttaaa aaagtttgct atgggtcaaa ggattataac
    35221 aattcaggga gataaaaaaa ttttaaataa tgctttttta gcacaatata tgcaaacaaa
    35281 agcttttagg gtagaaatag ataaatataa aactggtagc tctgcacaag gaattagaag
    35341 caaagaactt caaaaaatat taataccagt accatcaata gaacttcaaa ataagtttga
    35401 agagtttgtt aatcaagtag acaaattgaa agtaggaata aaaacaagtc taaaagaact
    35461 agaagacaac tttaactcat taatgcaaaa agcttttaaa ggatagttat tcaactaaaa
    35521 tacaattatt aaaatggggg aataaaatct atgaatcaaa tagagctaga tttataccat
    35581 aacatggtaa atatctataa agaagctgat ttacaatgta actataagcc aacaagattt
    35641 ttacaactga taaataacaa aggagcctta ttagctgcta aagaattaat caataaagaa
    35701 ggagctactg aaggatttac aaggctatgg gagtgtaaga gattagattt atcattagaa
    35761 gtattagtat taaaagaaaa gtataaagag ttgtttactg atgaagaaag agaaatatgt
    35821 ataaatagat taaaagatta tggatataaa ttcaattaag caatattacc taataaacaa
    35881 gaggtgatta aatgagaaat ataccaaatg agattaaaaa ctcaataata aatgtatttc
    35941 caaaagaaaa tactctagca atcataatat ttggtagtta tggaacagat aaacatacat
    36001 cagaaagtga tatagacata gcttggattc ccatcaaaaa agtaccaatt acagaacttg
    36061 caactaagac agaagctcta agaaatatat tagacattga tgtagaccta aaaattgtta
    36121 cagataatta tacaatagca cttaaaaaag ctattttaga aggtgaagtt atttataaat
    36181 ctaaagattt taaggattat ataaataaat tctattttga aaacagtgat gtaatagata
    36241 tcttagattg gagggacatg tatggtaatt agaaacaaat tatcttggaa ctcaatagaa
    36301 gaagtaaata gggattttgg ttcttgcttg tatgacctgc aaaacttcaa aatcttatat
    36361 gataaggaag aaatgcctga gttatgggct agatacataa aagaaggctt taaaagctat
    36421 atggtatcat ttctagaatt aacaaaagca atgttgtatt ataaatcaat tgatttaaac
    36481 ataaaatcta aaaactttta tgactacctt ttagcttgtg agtatcacaa tttattacct
    36541 aaaaattctt ctatagttat agaaacactt agaaaattga gaaatgatga ttctcatgga
    36601 tatgatatac cacagtttga agatatgtac gaattgttta cagaaaatga agaagtattt
    36661 gttagtataa gaaacagctg taagaaatag tttttagaga gatttatttt aactaaggga
    36721 caaaaggggg ataaaagcct tgggtaattt tgatttttta caaaagaaga aaaatttcaa
    36781 tagttttaat actgcttgct tagaagcaga aaaaagtata ctagttagcc catctacttg
    36841 tgcaactata acaagaagag ctttagagct tgcagtaaaa tggctatatg ctaatgatag
    36901 tgatttagta ttgccttatc aagataatct tagtacgttg attcataata atagttttat
    36961 agaacttata gactatgata tgttaccttt attaaaatat atagttaaat taggtaattt
    37021 atctgtacat actagtgcga acatagagag aggagaagca atactttcac ttaacaattt
    37081 gcatcaattt gttagttgga tagactattg ttattcagat gaatatacag cagaagactt
    37141 taatgaagat ttattacttc atggggaaga gaaaaggacg agaccagacg aacttaaaaa
    37201 tctttatgag agattaagtt caaaagataa aaagcttgaa gaagtaataa aagaaaatga
    37261 ggaattaaga aagtcactaa ctgaaaagag aaaagttaat actgtaaact atgatttcaa
    37321 gatagacgaa gtaagcgaat ttgagacaag aaaaagatat ataaatatag aattaaagct
    37381 tgcaggttgg gaatttggaa aagatatatg ggaagaaata gaagtacaag gtatgcctaa
    37441 tgaaagtggt gtgggatatg tagattatgt tctttatggt gaaaatggta agccattagc
    37501 agtagttgag gcgaaaagaa cgagtaaaga cccgaaaatt ggtcaacaac aagctaaatt
    37561 atatgcagat tgtttggaaa agagatatag tcaaagaccg gtaatatttt tgactaatgg
    37621 ttttgatatg tatatttggg atgactattc agataggaaa gtttatggat tttataaaaa
    37681 atcagaatta caacttatga tagatagaag aaagagtaag aagtctttaa gttctgtaac
    37741 aataaatgat gaaatatcca atagatatta tcaaaaagaa gctattaggg ctgtatgtga
    37801 ggctcttgaa aataagcaac gcaaaactct tttggtatgt gctactggta caggaaaaac
    37861 aagaattgct atatctatag ttgatgtact ttcaagacat aattggatta aaaatatatt
    37921 atttctagca gatagaaaag cattggtaaa gcaagctaaa aaaagtttta ctaagctatt
    37981 acctaacttg gcgttatgta atttattgga tagcaaagac agcccagaag atgctaggat
    38041 gatattttct acatatccaa caatgatgaa tgcaattgat gatacgaagt ctaaggatgg
    38101 aaagagattg tttacacctg gtcattttga tttaattata atagatgaat cgcatagaag
    38161 tatttataaa aagtataagt caatctttga ttattttgat tcttacttaa tgggtcttac
    38221 tgctacacca aaggatgaga tagataaaaa tacttattca gtttttgaca tggaaaatgg
    38281 agtacctaca tatgcttatg agtataataa ggcagtcgag gatggatatt tagtagatta
    38341 tactagtatt gagtttaaga ctaagattat ggaagatgga attaagtatg atgagctttc
    38401 tgatgaagag aaggaagaat atgaaaatac atttaatgat gatgagagta ttggagacga
    38461 aatagggaat aatgcagtaa atgagtggct atttaattct gacacaatag atttagtttt
    38521 aaataagcta atgacagagg ggttgaaaat agaaggagaa gaaaagattg gtaagactat
    38581 aatttttgca aagaatacaa agcatgctag ggcaatagtt gaaagattta ataaactgta
    38641 tccaaagtat ggtgggaact ttgtaaaagc agttgactat agtataaatt atgtagattc
    38701 tataatagat gatttttctg ataagaataa attacctcaa atagcagttt cagtagatat
    38761 gcttgataca ggaattgaca tacctgaaat actaaatctt gtttttttca agaaagtgag
    38821 aagtaagact aagttttggc aaatgatagg tagaggaact agattatgtc cagatttatt
    38881 aggtgttgat atggataagg aaagattctt aatatttgat ttttgtaata attttgaatt
    38941 ttttaaattt aatcctaaag gatttgaggg aaataaaaca gaaacactta ctgaaaaatt
    39001 atttaatata aaggtaagta tggttaaaga acttcaagat attaaatata tagaaggtga
    39061 gtatgcagaa cttagaaaag aattgttaga agaacttata acttctgtaa aagctttaaa
    39121 tgaggatagt tatatagtta ggatgaattt aagttatgta cataagtata aaaatgaaaa
    39181 tgtttggagc aatattggag cagtagcaca aaatgagata agagaatata tatcaccact
    39241 cataacttca tatagtgatg atgaattggc taaaagattt gatgtagtta tgtataatat
    39301 acaacttgca tacttacaaa ataataatgc aagtaaaggt ataaggcatg taatggctac
    39361 agcagaaaaa ctttcaaaac ttgggactat accacagatt cagcaacaaa aatatactat
    39421 tgaaaaagca atgacagagg atttttggga agattcagat atatttgagg tagaagaagt
    39481 acgtatatca ttaagagagc ttattaagta tcttgaaaag tctagtcaaa agatatatta
    39541 tacaagtttt gaagatatga ttgtagcaga agatagaaat gattctgtat ataatgcaaa
    39601 caatcttaag aattataaaa agaaggtaga gtattattta aatagccata aagatgaatt
    39661 agcaatattt aaattaagaa ataataaaaa gataacaaag caagatgttg aaacgttgga
    39721 ggaaatactc ttaaaacagt taggtaattg tgatgattat aagaaggagt ttggagatac
    39781 tccagtaagc caattagtga gaagactagt gggattggat agagaagctg caaatgaagc
    39841 tttttcagag tttttaaata ataagagctt taatactaag caaatacatt ttgttaaact
    39901 tatagtcgat tatgtagtca agaatggatt tatagaggat aacaaagtgt tgatggaaga
    39961 cccatttaga actgttggaa gtataattga tttatttgaa aatcatattg aagagagaaa
    40021 taaattgatt aaaactataa ataaaattaa agaaaatgca tcagagatag gctaatgatg
    40081 ttatttagtg caaaattatt gttaatgatg tagagaataa atttattagt aggtagtcta
    40141 atattgggtt gaaatagata actagatatg gataatgtaa atatacaaag agaggtattg
    40201 aataattatg caatatctct ttttaattga taaataacta tgaattactc atgaagaaga
    40261 ttaaataata atttttaaaa ttaagtattt gtatatttta tagaataatc atgtatttac
    40321 tttaataaaa taaaaacagg tataaaaagg tatataataa ggctttcata tacatttatg
    40381 agtcaaaatt ttgatataat atatagatac atggagttat gaatggaatt tatttaactt
    40441 atagggggag ttaatatgaa agcaggagaa attgaatttc ttagttattt agaggggtca
    40501 aacaaaagtt ttgtagtacc tgtatatcaa agaaattata actggaagaa agaacaatgt
    40561 aaaagattgt ttgatgattt agaggatata atagattctg gatttagaac tcatttctta
    40621 gggtcagttg tatctatata tagtatggga aaagaatatt taataataga tggacaacaa
    40681 agagtgacta ctgtgtcaat attattgatt gccatgtgtc aaataataaa agagtttggg
    40741 gaatcaaaag gaattattac aacagaagaa cagattaagg agtgttatct tataaacaag
    40801 cactcaagag ataaagatgg aaaaataaag ttaaaaccaa tagcagaaga tagaaaagcg
    40861 tactatgaac tatttgaaga agaaatgaat acagatagaa attctaatat agtttcaaat
    40921 tatttatatt tttataatag aataaaagaa gataatatat caatagatga tttgtttgat
    40981 gctatacaga aattaactat agtagaaata gagttaaaaa atggtgaaga tgaccctcaa
    41041 ttaatctttg aaagtctaaa ttcaactggc ttagatttaa gtgaagctga tagagtaaga
    41101 aactttgtgt taatgggtag aagctctaag ttacaagagg aattttataa taattattgg
    41161 tataaaattg aaaaaaatac aagtagcaac gtaagtagtt ttataagaga ctatttgact
    41221 ataaaggaga gaagtatacc gaataaaaat aaagtatatt ttagttttaa aaggtatgta
    41281 tcagataatg gtatagatat a
//
LOCUS       DAEMGG010000022        40916 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEMGG010000022 DAEMGG010000000
VERSION     DAEMGG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.022"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            179..1096
                     /locus_tag="KPK24_001132"
     CDS             179..1096
                     /locus_tag="KPK24_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG2298837.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            1174..1911
                     /gene="cas6"
                     /locus_tag="KPK24_001133"
     CDS             1174..1911
                     /gene="cas6"
                     /locus_tag="KPK24_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG2298838.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            1917..3767
                     /gene="cas8a1"
                     /locus_tag="KPK24_001134"
     CDS             1917..3767
                     /gene="cas8a1"
                     /locus_tag="KPK24_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBG2298839.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNMIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFVKENKKIQSIYDYKLKSN
                     VLDLILKSKDLKHLINNTLRNIVKSNLESNNKSNISGLSCFNVVQLRTILNNYKRGVY
                     KMDYKKLRKIYESGCEIHDYYIDKNAKNKIEGITYKILNLIKVGNKNDFMQTILRIFS
                     PSEKTPPEEILQIFVEEDLDFESVGYAFVTGLISGKYEGKDKLPNEKEEK"
     gene            3769..4683
                     /gene="cas7i"
                     /locus_tag="KPK24_001135"
     CDS             3769..4683
                     /gene="cas7i"
                     /locus_tag="KPK24_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG2298840.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            4685..5386
                     /gene="cas5b"
                     /locus_tag="KPK24_001136"
     CDS             4685..5386
                     /gene="cas5b"
                     /locus_tag="KPK24_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBG2298841.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            5397..7718
                     /locus_tag="KPK24_001137"
     CDS             5397..7718
                     /locus_tag="KPK24_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG2298842.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTYLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFANSDRNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            7733..8284
                     /gene="cas4"
                     /locus_tag="KPK24_001138"
     CDS             7733..8284
                     /gene="cas4"
                     /locus_tag="KPK24_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBG2298843.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            8287..9267
                     /gene="cas1b"
                     /locus_tag="KPK24_001139"
     CDS             8287..9267
                     /gene="cas1b"
                     /locus_tag="KPK24_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBG2298844.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            9270..9536
                     /gene="cas2"
                     /locus_tag="KPK24_001140"
     CDS             9270..9536
                     /gene="cas2"
                     /locus_tag="KPK24_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBG2298845.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   9720..11448
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9720..9748
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            11509..11937
                     /locus_tag="KPK24_001141"
     CDS             11509..11937
                     /locus_tag="KPK24_001141"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG2298846.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(12392..13171)
                     /locus_tag="KPK24_001142"
     CDS             complement(12392..13171)
                     /locus_tag="KPK24_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298847.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            13495..14040
                     /locus_tag="KPK24_001143"
     CDS             13495..14040
                     /locus_tag="KPK24_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG2298848.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            14072..15208
                     /locus_tag="KPK24_001144"
     CDS             14072..15208
                     /locus_tag="KPK24_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG2298849.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            15212..16603
                     /locus_tag="KPK24_001145"
     CDS             15212..16603
                     /locus_tag="KPK24_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG2298850.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRIIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            16679..17554
                     /gene="dpsA"
                     /locus_tag="KPK24_001146"
     CDS             16679..17554
                     /gene="dpsA"
                     /locus_tag="KPK24_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG2298851.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            17557..18147
                     /locus_tag="KPK24_001147"
     CDS             17557..18147
                     /locus_tag="KPK24_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG2298852.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(18354..18554)
                     /locus_tag="KPK24_001148"
     CDS             complement(18354..18554)
                     /locus_tag="KPK24_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298853.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            19058..21712
                     /gene="adhE"
                     /locus_tag="KPK24_001149"
                     /gene_synonym="adhC"
     CDS             19058..21712
                     /gene="adhE"
                     /locus_tag="KPK24_001149"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG2298854.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            21988..24207
                     /locus_tag="KPK24_001150"
     CDS             21988..24207
                     /locus_tag="KPK24_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2298855.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFVAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            24461..25123
                     /locus_tag="KPK24_001151"
     CDS             24461..25123
                     /locus_tag="KPK24_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBG2298856.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            25494..26903
                     /locus_tag="KPK24_001152"
     CDS             25494..26903
                     /locus_tag="KPK24_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG2298857.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            27018..27293
                     /locus_tag="KPK24_001153"
     CDS             27018..27293
                     /locus_tag="KPK24_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG2298858.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            27923..28234
                     /locus_tag="KPK24_001154"
     CDS             27923..28234
                     /locus_tag="KPK24_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298859.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            28236..30161
                     /locus_tag="KPK24_001155"
     CDS             28236..30161
                     /locus_tag="KPK24_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG2298860.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVIFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            30164..30655
                     /locus_tag="KPK24_001156"
     CDS             30164..30655
                     /locus_tag="KPK24_001156"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG2298861.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            30707..31270
                     /locus_tag="KPK24_001157"
     CDS             30707..31270
                     /locus_tag="KPK24_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG2298862.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            31285..32262
                     /locus_tag="KPK24_001158"
     CDS             31285..32262
                     /locus_tag="KPK24_001158"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG2298863.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            32249..32572
                     /locus_tag="KPK24_001159"
     CDS             32249..32572
                     /locus_tag="KPK24_001159"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG2298864.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            32689..34467
                     /locus_tag="KPK24_001160"
     CDS             32689..34467
                     /locus_tag="KPK24_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG2298865.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            34464..35837
                     /locus_tag="KPK24_001161"
     CDS             34464..35837
                     /locus_tag="KPK24_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG2298866.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            35843..36511
                     /locus_tag="KPK24_001162"
     CDS             35843..36511
                     /locus_tag="KPK24_001162"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG2298867.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            36788..37837
                     /locus_tag="KPK24_001163"
     CDS             36788..37837
                     /locus_tag="KPK24_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2298868.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            37933..38922
                     /locus_tag="KPK24_001164"
     CDS             37933..38922
                     /locus_tag="KPK24_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2298869.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            38927..40645
                     /locus_tag="KPK24_001165"
     CDS             38927..40645
                     /locus_tag="KPK24_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG2298870.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLFTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(40727..>40916)
                     /locus_tag="KPK24_001166"
     CDS             complement(40727..>40916)
                     /locus_tag="KPK24_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2298871.1"
                     /translation="NNIINNINLKIVEENSTNELKLSMSIGYAIFDEKVDVSLFDTYR
                     RADSQMYDNKRKINKQYQ"
BASE COUNT        16192 a         4375 c         7076 g        13273 t
ORIGIN      
        1 attaaaattt tgtacaaata taaaatataa tatggtaaat gtcaaaatta taaaaaaatt
       61 ctacttatat aaaatataaa gaggttatat gtacatatgt gtagaaataa ataataaaaa
      121 tatcaattaa tgttattagg gggggttact cattagtaaa ttagctaata cacttagaat
      181 gatgatatta attcaaagca gaggcaaaat gaaatgcaaa gaattatcag aagagttgga
      241 agtaagtgaa aggcaaataa aaagttacaa gatgtattta gagcaagctg gtatatttat
      301 aaattcaaca cctgggattt atggtggtta tgaaatagat aaatgtaata gcatctcatt
      361 aattaaatta ttagatagtg aagtttcaat tctagatatg ataaattcac agttagagta
      421 taataatgat atttacaaaa atgaatttaa taatattgta gaaaaaataa aagcagtatt
      481 aaatacagga gaaaaaagcg atacatatat ggattatttt acagttcaag cacaacgtaa
      541 ttgtgattat gagtctgaaa aaaataagtg taatgaaata attagagcat ataccacaaa
      601 acacaaattt tggatagaat attattcatt aaattcagga aatagtgaaa gaattgttca
      661 tccatatggt ttatttaatt ataaatcaga tacttatatg gtagcttttt gtgaaaaaag
      721 atttaagttt atagacttta aattatgtag gattaaagat tataaggttt tagaagaaaa
      781 atataatgta gataaaagtt ttagttggga tgaatatagt aaaaatagta taggaattta
      841 taagggagaa gaaattaatg ttgttataaa aattagtcat ccattttcaa caattataaa
      901 ggagaaagtt tgggttaata atcaacaaat catagaatat gatgataaat ctataatgtt
      961 taaagctaaa atgagaggtt atgaggagat aaaaagttgg attttaagta tgggagctta
     1021 tgttgaggtt gtagaaccag atagacttag aaatgatata ttgttagaga ttgaaaaaat
     1081 gaaaaaaatt tactgatttt ttcaaataat ttatacttgg accaatatac ttcacggtct
     1141 agtgttaata tattaattga gagaggtgat attatgaaaa tgaaaattga atttagtaca
     1201 ggttgtatac caatatctta taattcgctc tttatgagta ttataaaaga agcaataaga
     1261 aaatctaatg aagattacta taaaaacatg tactattata aagaaaagaa taataaaaaa
     1321 actaaaaatt ttacattttc ggtttatata aaaaagtata gtattgaagg tgacaatttt
     1381 attatagaag ataaagtaat attaaatata agtactccag atttagaatt gggttttcat
     1441 atatacaatg ggttaatgac ttctaaaaaa tgtttatata aagactacga acttacaaga
     1501 attagaatcg atttatcaag agaaaagaag gtaacagaag aaagggtact atttaatgca
     1561 ttgtctccaa tctgtgtaaa gtctaaagaa ggaaaattct tggagataac tgatgataga
     1621 tacatagaag aactaaacta tataactaat gaagtggtta aaaattatag ggggaatgga
     1681 ttaaaaagaa agttagaatt tgaaaatata ggtctcaaaa aggtagtagt taaagagagt
     1741 ttaagagaat ttaaaaaaat aacaggtaaa gagtatcaat atattaatgg ttacaaaggt
     1801 aaatttatat tgaaaggtga tattgatgat ttaaatctta tctacaatct aggaattggt
     1861 tttagacgtg ctcagggatt tggagatgta gatattttag aatggaggtg agataaatga
     1921 agttaagagt ttatagagga gattggtttt ttaatatggg tatagttgga tttttaaata
     1981 taataaagaa agcagataaa caagctgaaa tatttattat ggaagattat atcgaatttg
     2041 atagcttatt tcttgaaaac tttcatgagt attattttaa ttactttatg gatgaatatg
     2101 atgtatcaaa aagaattaaa aaaaatattg attacagtat aaattttatt aaatctaagc
     2161 ctgatagaat caaagatgga attaagaaaa ttaaagatag tgtgaaacaa caaaatgata
     2221 agattaaaaa atttgatgaa gaaaaattta acatgataaa agagaagcta gatagtatgt
     2281 ctaagataaa aagttatgat gaatttgata gtttagaagc tttagtagat gagactatag
     2341 atatatttaa aataaaatca ataaatgaca ggcttacagc aaatctttat aaatatgtag
     2401 ttcaagataa ttattttgga caagttagtt tttttaatgt tgctaaggcg aaattagatt
     2461 tagatggatt aaaacaggta atgtttaatg attatttaag acaaattata tattttggag
     2521 agttagaaga tttattagaa gagaatgact atgacaagtt gaaaaactat ttaaatgata
     2581 gattaaatag tatagctaag gatataaatg aaaaaaaagt tagtaagtct agtataaata
     2641 ctatagaaaa aattatgaag gaaataaata aaaactttat aaagaaaaat aaaagcatag
     2701 aagatataaa aaagtatatg gatagtttag aagtatgtga aatgtgtggt ttatataagg
     2761 gaatattaga tgaatactca gaaagtaatt ttgctccact tggagtaagc accaataatg
     2821 ctaggaatat gttttggaat caggcgtatg taccatcaat atgtgatatt tgtaagctaa
     2881 tattgttctg tactccagca ggggctactt atacaagaaa gaattattta attaatgaag
     2941 aaaatgagtt ttatttattt gttaacatgg atacttgtat aaatgagctt tttgaaagaa
     3001 ataatagttt aaaagctcaa aaaagtgagt attcagattc caaagatgaa aatcctttta
     3061 atcagcttat aaaaagtata gtagaagaaa atacattaaa aagtgaatgg caacttcgta
     3121 atatactttt tgtggaattt aaagcaagta tagattctaa aaaatgtaaa ataaattatt
     3181 ttaatattcc aacttattta gcaaagtttt ttgtaaaaga aaataaaaaa atacaaagta
     3241 tatatgatta taaacttaaa tcaaatgtgt tagatttaat tttaaaaagt aaagacttaa
     3301 aacatttaat taataacact ttaaggaata tagttaagag taatttagaa agcaataata
     3361 aatcaaacat atcagggtta tcttgtttca atgttgttca attaagaaca atattaaata
     3421 actataagag aggagtgtat aaaatggact ataaaaagtt gagaaagatt tatgagtcag
     3481 gttgtgaaat acatgattat tatattgata agaatgctaa aaataaaata gaaggaataa
     3541 catataaaat tttaaattta attaaggttg gtaataaaaa tgattttatg caaacaattt
     3601 taagaatttt ttcaccaagt gaaaaaacac caccagaaga aattttacag atatttgttg
     3661 aagaagattt agattttgaa tctgtagggt atgcatttgt aacaggatta atttctggaa
     3721 aatatgaagg aaaagataaa ctaccaaacg aaaaggagga aaaataagat gaaaagaggg
     3781 ttaacattta cagtaatagc acaggctcaa agtttaaact atggggaagg gataggtaat
     3841 atatcagaat taaaaaaatt aagtagagat gatggaaata tatatacatt tgcatctcgc
     3901 cagtgtttaa gatatgacat agtacgatta gcagcagaat tgtttaattg gaatttacag
     3961 gttgtcgata aagaaaaagg cactattcaa tttaaagaca atataagtat aagagaatca
     4021 gaagaaatgg atttatttgg atatatgaaa acaaataaaa aagatgagaa agataaaaaa
     4081 ggaggttctt tgactagaga agctactgta agattaagta atgcaatttc ccttgagcca
     4141 tatagaagtg acatggactt tttaaataat aaaggattag cagatagaat aggagaacat
     4201 cctaatcttg ctaacataga gcaacattta agttattata catatacagt gactatagat
     4261 ttatctaaaa taggtaagga tggagatata gaacttgata ataaagagaa atgtagaaga
     4321 gtagtagaat ttttagaaat aataaaagta ttgaatagaa atattagagg tagacaagaa
     4381 aatttatctc ctctatttgt agtaggagga gtatatgaaa tagcaaatcc atttttcttg
     4441 ggtagaatta aattaaaagg agataaaaat ggatttaaaa taaataagca agctatagaa
     4501 gaagtaattc aaggtacttt tttaggaaaa gatttaaaag aatttaccta tgttggaatg
     4561 gtagatgggg tatttataaa taaagaagaa tttaagggat tgtttgaaga taatttttta
     4621 agtgtagata aattttttgg acaactagtt aaagaagtaa aggaatatta tggggtgaat
     4681 taatatgaag gttttgaaat tagatttatt tcaagaaact gcatgctaca aaaaaccatt
     4741 tgctatgaag attacagaga catatccact gcctccatat tctacaataa atggattaat
     4801 tcacaaaata ttaaatgcta cagaatatat accatttaat ataagtgtac agggaacata
     4861 tgaaagtata tttaataatt accagacaac atatttttat aaaaaagata gtattacatc
     4921 tatgccaatg aatagtcata tgctattgaa tgtaaatttg ataattcata taggtgggga
     4981 ttttgattta ttggaagaaa tatatgatag tttattaaat tatgacgagc acttatcatt
     5041 aggtagaaaa gaagatttag taagaattaa tgatattaga tttgtagaag tcaataaatt
     5101 tgaagtagat gaggatatac aattagatga ttatgaaaat tataagttat cagaatgtaa
     5161 tataagaatg cctatatata taccaaaaac aagtttacaa gatacagata tagatggtat
     5221 aagttacaga ctcaataatt actatgataa taatgctgtg gaagataaaa aaagggtttg
     5281 gaataaggta gatagttatt atgttgagga tggaaatact atatcaattg gagatatatt
     5341 attagataat gaaggagatt tattatattt taataatata aattaggagg gagattatgt
     5401 tatatgctaa atctaatcca gtggaaacac ttagagaaca tactgatgaa cttttaaaac
     5461 aaatgaatgt attaagagaa agttatggaa aaaatataaa tagcttagat tttctagaag
     5521 aggagatatt ttggcaatta cttgactttg ttatagaatt tcatgatatt ggaaaagcat
     5581 tttcaccgtt tcaagagtta ataaaaagca gaatggatac taaaataaat gaacctaaaa
     5641 tagtaactta tttagaaaat aatgtaggcc ataattatct ttctcctgca tttattgatt
     5701 atagttatat agacaggaaa aaaaataaag aacttagagc agtattaaat caagttattg
     5761 tatatcatca tgaaagagat atctttatag ataaagattt taaaaattta atacaaaaaa
     5821 tattagatga agatttaata aataaagttt atgaactaca gcatgaattt aaagttagat
     5881 atccaataaa aacagaaaaa ttgagtaaag tatatctaca aagtgtagaa aaaagaatag
     5941 ataaaaatca caagtactat aatttatata ttatgttaaa aggaatacta catagactag
     6001 accatagtgc ttctgctcat gaagttgtgg aatgcaataa tgttataaat ataggagaac
     6061 agacagaaaa ttatttaatg aaagagtttg gtagtttgag agaggctcaa tcatttgcta
     6121 aatctaatag aaataagaat ataatactga ttgcatctac aggaatggga aaaacagaaa
     6181 ctgccttaat ttggatagat aaagacaagg cattttttac acttccatta agagtaagta
     6241 taaatgcatt atttgataga gcaaaaaata tagttggtgt tggagaatca aatgatacat
     6301 tcttaggttt gttacattca acagcaatag attatctaga agaaagtaat caagaaaatt
     6361 caagtgagat tgtagattta gcaaagttat tatcttgcaa actgacattt tctacaatag
     6421 accaaatatt taaatttcct tttttgtata gagggtatga aaaagtgtac tcaacattag
     6481 catattcaaa agtagtaata gatgagatac aagcatattc accagaaata gcagctgtat
     6541 tggtaaaagg tatagaaatg atacataaga taggtggcag atttatgata atgacagcta
     6601 ctatgccaac catatatata gatgaattaa aaaaaagagg tgtaatgaat agtaacttag
     6661 ctgaattgac atgtaataca gaaaaaataa gacattgtgt ttctattgta gaaaattcaa
     6721 tagatgaaaa cttaggtaaa ataatacaaa gtggaatgaa taaaaaagtt cttgtaatag
     6781 taaatacagt taaaagtgct gtagaaaaat atgagttaat agaagaaata gttaaaccaa
     6841 aaggaataga tgtcaactta aatcttttac attctatgta tatacaagaa gatagagcaa
     6901 aattagaaaa atatataaaa gaatttgcca atagtgatag aaatggaatt tggattacaa
     6961 cacaattagt agaggcatct cttgatattg attttgatga gcttcataca gaaaattcaa
     7021 cacttgacag tctatttcaa cgatttggta gatgttatag aagtcgtgag tatgaagaaa
     7081 atagcccaaa tatatttata tatacagaaa aagctacagg aataggtagt atttatgata
     7141 aggatattgt agaaaaagga cttgagctac tgaaaacttt tataaatgga aaagaatgtg
     7201 tgaagatgaa agaaaaatat aaggtagaaa tggttaagat actatactct aaagaaagtc
     7261 tagaaggtac agcttttgaa aagagattta ccagtgcttt aaatatttta gatactataa
     7321 cgccatatag tttaggttct aaagatgcac aaaatatact tagaaatatt gaaggatata
     7381 cagtaattcc agaggatata tataataaaa ttgaagaaac tttaatcaaa gattatgaag
     7441 agttgggaca agagcttata aatgcatata gtaatgatga tagacaattg ataaataaaa
     7501 taaagtctaa gagaaaaaaa gcaagaagag agataattaa aaaaactgta aatttaccaa
     7561 tatataaagc taaacaaaat gtaattgaca tacaggtgaa aggtcttgaa gatttaaaaa
     7621 tacttttata taaatatgat atatacgaaa ataagcatac aaaacagata tttggtaaag
     7681 gtgtgcttat aagtaatgaa atagaccaat ttatttgatt tttatattaa caatgtggct
     7741 aaaatttact aaagaaggtg attttgtgcc aatagattta gaagtattaa aagttcaagg
     7801 tgtaaaaata aattattatt atgtatgtaa aagaaaacta tggctatttt ctaaaggaat
     7861 aaccatggaa caaaatagta gtagagtatt gtctgggaag atagtacatg aagattcata
     7921 taaaagaatg aaaaataaag aaatattgat tgatgatatc ttgaagctag atataataaa
     7981 ggatgagtat gtaaaagaag taaaaataag ttcaaagatg aaagaagcag atgaaatgca
     8041 gttatactat tacttatact atttaaagaa tagtatgggt atagagaaaa aaggtactat
     8101 aaattatgtt aaggaaaaga aacaagaaga attgatattg acagaagaaa aagaaagaat
     8161 tattgaaaac acacttataa atataaatga aataaatatg cagttatctc ctcccaaaat
     8221 tgagaaaaaa cattattgta caaaatgtgc ttattatgaa ttttgttttg tgaaggagga
     8281 gtaacaatgt ataaagacta ttatattata agtaatgggg atataaaaag gaaagaaaat
     8341 acgatttact attacaattt agaaggtgag aaaaaaatat taccagtaga acaagttgat
     8401 gatattcata tatatggaga agtagattta aatacaaaac ttataaatta cataagcaaa
     8461 tatggggtga tgcttcattt ctacaattac tatggatatt attcaggtac atattatcca
     8521 aggaagaaaa atgtatcagg attcagttta gtatgtcaat cagcctgcta tttagataaa
     8581 aatgaaagat tatacttagc aaaatccttt gtggaaagtg catcatacca tattttaaga
     8641 aatatgagat attacaaagt tgatgaaagt ttaattgata ctataaagga tgaactaaat
     8701 gatttgaatg atgtcaaaga aatatcagaa cttatgggta aagaaggcag aataagaaaa
     8761 acatactata aatcatttaa tcaaatatta aaagatgatt ttgaatttta taaaagagaa
     8821 aaaagacctc ccaaagaccc agtaaatgct cttatgtctt ttggaaatag cataatgtat
     8881 acgaatgttt taagtgagat atataagaca caacttgacc ctacaataag ctttttacat
     8941 gaaccatcaa caaagagatt ttctttaagt ttagatattg cagaaatttt taagccaatt
     9001 ataattgaca caataatttt tagcctgatt aataataaaa gaattaataa gaatgatttt
     9061 gaatatcaag acaaaatttg ttatttaagt gaaaaaggaa agaagaaatt tttgcaagaa
     9121 tttgaaaata agatgcagac aactataaaa catagaaatc taaatagaaa agtttcatat
     9181 agaatgttta taaggttaga gtgttataaa ttaattaaac attttataaa tgatgaaagg
     9241 tacaaagttt taaaggtgtg gtggtaagta tgtttgttat tgttacttat gatattgttg
     9301 aagcaaggtc gttaaataga attagaagga tacttagaaa atatttgact tggacgcaaa
     9361 attctgtttt tgaaggcaat attactgaag gaaagttaca taaatgtatt tctgaaatag
     9421 aaaatattat tgataatagc gaggattcaa tctatgttta tgagataaaa aatcctaatt
     9481 caattaaaaa gaaatgttat gggattgata agtattctga tgaaatgttt atataggttt
     9541 gcagtgagcg atatttatgc taaaataggt gttaacagtt ggaatataag ggattgaagg
     9601 tgtatgataa ttgttatcaa ttgcactact gctcgctcac tgcaaatttt gatgttttta
     9661 ttgaattata attgcttgat tgaagtgttt tcaatgtatt caaatatacc tattttgggg
     9721 ttttatatta actaagtggt atgtaaataa ttttttcgga tatttaataa tttattttaa
     9781 atttagtttt atattaacta agtggtatgt aaatctcaac agtatctaca agaacttcta
     9841 tatcttctaa gttttatatt aactaagtgg tatgtaaatt aatttagggt aacttttata
     9901 ttattttctt ctatcgtttt atattaacta agtggtatgt aaatttggtt gatatagata
     9961 ttagtcctat tatttctgct tgttttatat taactaagtg gtatgtaaat aaattagata
    10021 atttataagt caataaaata agtccaagtt ttatattaac taagtggtat gtaaataaat
    10081 ttgtaatatt atataatcat cctaaaacaa aaggttttat attaactaag tggtatgtaa
    10141 attcgaatgc tataatttta atatataaaa taaatggagt tttatattaa ctaagtggta
    10201 tgtaaatatg gattttagtc gtttttaaat ttttctcgta atagttttat attaactaag
    10261 tggtatgtaa ataccattca tttatatttt tatctgtatc tcttagccat gttttatatt
    10321 aactaagtgg tatgtaaatt aagttttctt aaaaaagtac ttgctttaaa tatgttttat
    10381 attaactaag tggtatgtaa attttatttt ctctttatta gtcattagtt tttgccttcg
    10441 ttttatatta actaagtggt atgtaaatat gaagaatttg cgaaactaat aagaaaagat
    10501 attgagtttt atattaacta agtggtatgt aaatctttct ttatcaaagt aagctaaatt
    10561 tagttcctcc gttttatatt aactaagtgg tatgtaaatc gtcattactt tttattgctt
    10621 ccattaaatt tgttcgtttt atattaacta agtggtatgt aaatatcgca agataggcta
    10681 aatctatcaa ggagagttga gttttatatt aactaagtgg tatgtaaatg actctgccat
    10741 ccctgtaaca aacagtctta ccgcaagttt tatattaact aagtggtatg taaataaata
    10801 ttttacgatt agttgatgct ataattctat tagttttata ttaactaagt ggtatgtaaa
    10861 ttcttttgat ttagcgttgt ttgtccggaa ttttaaagtt ttatattaac taagtggtat
    10921 gtaaattcgg gctagaatgt attttaaacc ctagttcatt gaagttttat attaactaag
    10981 tggtatgtaa attttctaat atcgctattt aaactcaatg cttcatttgt tttatattaa
    11041 ctaagtggta tgtaaattca gaacaaatta aatatgtttc gttttccatg taaggtttta
    11101 tattaactaa gtggtatgta aatagaactt ttttatatta gaagtacttg acatgattgc
    11161 cgttttatat taactaagtg gtatgtaaat cttgataata tttcagcata tgatactgaa
    11221 gatggtttta tattaactaa gtggtatgta aatttgccaa tgtaacatct ttagatatta
    11281 cagtatcatt gttttatatt aactaagtgg tagataagta gaatattagc aatatcaacg
    11341 agtatttaga aacttatttt atattaataa agcagtatat aaaattgtag aatcaacaat
    11401 agcatatact aaaacatcca ccttatatta actaaatcat atctaaataa aataaactta
    11461 cctataaaca ttataaaatc aatacaaaaa tgaggtgaaa taaaatttat gataaagaaa
    11521 ttaaacaata aagatataaa taaaatcatg gaaatatggg aaaaaagtac aatcaaagca
    11581 catgacttta taagtaaaga atactggcaa aataactata atactgttaa aaacgaatat
    11641 atacctatat cagatacatt tgtatatgat gatggagatg aaataaaagg atttataagc
    11701 ataatagata aaagctttat aggagcttta tttataaagc ccaaatacca aaatctaggt
    11761 atcggaggta aacttttaga ttatgcaact aaaaaatata aaagtctaag cttagcagta
    11821 tataaagata ataaaaaagc agttgtgttt tataataaaa aaggttttaa tatagtaaaa
    11881 gaacaagtaa atgaagattc aggatttaaa gagtacataa tggaatatag taaataatat
    11941 gattacattc tattaaaagg ataattaatt tatttgccat ttgatttttt aagagtaatt
    12001 tttaaaatag actatatgaa gataaagata tttattatag atgtaagtta atcagaattg
    12061 atatagctac aaaagaggaa aataaaattg tatggtgtat ttcagattta acaaatttac
    12121 tatttaaaga taataagttt gatataatat taaagatact ttcacctata aactataggg
    12181 tatttgaaag aattttaaat aattatggaa tgctaataaa ggtttgttaa aatttaataa
    12241 ttattagatt atagaaaatt gccatgaaat tagttcatca agaactagtt tcatagcaat
    12301 tttaattctt ataagtgtat ttatttttac agatttaaaa aacttataca gtatctaata
    12361 atgtaatctt attactttag cctataaata tttaaatctt aaaatgttct ctttgagata
    12421 agtttatttc agagtcacaa tttccacaac agtaagatgt tgagacatct ttaaattttt
    12481 cagtattctc atcaaagttc atttttttct ttgcaataat tcctacctta gttaaattag
    12541 aaccacaatt attacagttt tgaattttgc ataggttata tagtttatca agtgtaatat
    12601 cttctaataa ttcatcacaa ttacaacatc tgatagaaga aacttcacta cacatttcaa
    12661 taggttcagc ttctttagaa gaaaatttat atgttatgtc atttgatact ttataacctg
    12721 tatcaaatat attagaatta caacttggac ataagtactt tggaagatat atatcttttt
    12781 tttcaaaagt atcactacta atattacagt ttaattcatc taaaattggt ctttcatcta
    12841 ggttattacc tttatttaag cttagaagat aattataaat ttttttatct tcattctgtt
    12901 ttgatgtatc taacaaatgc tccattatat tagtgtattt tttgtttttt gaaggcatgt
    12961 ttaaatccag ataattgttt tcaatagcaa agcatactat attgaaatag ttatatttaa
    13021 ctgcaaaaaa taataacatt tcatcaaata ctttatctat tattttgtca gaaatactta
    13081 tccaaaaatc tggtgactct aagtaaactt tcctactaag ttcatctaac tcttcatcta
    13141 tattatttat attattttca ttaactttca tatcatcact tccttttaaa tatcttgtgt
    13201 ataaactatt tgttactatt ttttatataa aataaaatct tatataaaaa tatatcatat
    13261 tataatttac aagtatagtc taaatagagc tgtttttaag caaagtttta taaatgacta
    13321 ttaaaatata aagtaacagt ataaagttta cttataaatt aaatgtaaac ctaaatgctt
    13381 ataatagttg acaaaaaaac gtctttattt tatactaaaa caaagcttta atagaagctt
    13441 tgtatatgta catataaact aaatgaaata agacatatga agggaattat aaaaatgaaa
    13501 aatactaaag taaatattca agatttaact aaaatgtcaa tatgtgtagc attattgtgt
    13561 atatcatctt acatatatat accattgcca tttactccag caggagtaac agcacaaaca
    13621 ataatgataa atttaatagc tttgatttta acaccaagac aagcatttag tacagttggt
    13681 gtatatataa tgataggttt aattgggatt ccagtatttg gtggaggtac atctggtatt
    13741 ggaaagttat taagcccaac aggtggtttt tattttgggt ttttgtttgc agcactaatt
    13801 attagtttat taaaaggacg aaataatgat attaagagat atatactaat aacaattttt
    13861 gttggaatgc ctattatata ttttatggga acagtattta tgtgctattt taatcaaatg
    13921 acagtagaag cagcactttt tgcagcagta gtaccatttt taattggaga tacaataaag
    13981 tcagttattg catctttttt aggcactaaa ctgaacaatg tactcagaag aaatctatga
    14041 taaatctata atataaggaa gggtgtaatg tatgaaggaa gtttttatat taggaggatt
    14101 gagaagtcat ataggtctaa aaaatggaat ttttcaattt gttcaacctg aattactagg
    14161 agcaagtgtt ttaaaagatt taattaaaaa gtatgaaatt gataaaattg atgagattat
    14221 atgtggaaat gcagttggga caggagggaa tatagctaga ttaatgactt taacagcagg
    14281 agtgtcaaat gaagtacctg cttttactgt ggatatgcag tgtgcttctg ctatgatgag
    14341 tatagacata gctttttcaa aggttaaatc tggtcagtgt gatttaatta ttgcaggtgg
    14401 ttttgaaagt agttctctac aaccaatgcg cacatatcat aagaatgata agcgatataa
    14461 tacaaataat ccaaattata cagttgctca attttctcct gatgataata gccaaaatag
    14521 tatgctcgaa ggtgcggaaa gagtagcaga gttatatcaa attgagaaag cagacttgga
    14581 tttttgggta aaggaaagtc ataaaagagc aaaagaagca agagaggaaa aaatacttga
    14641 agatattata tctcctataa ataatagtac ctatgatgaa ggtatacgtg ataagatgag
    14701 tcaaaggtta ttagatagga tgccatcaat tttaggaaaa gaaactatta caaatgcagc
    14761 aaattcatgt cttattaatg atggagcttc atttattata atatgttcta agaagtattt
    14821 agaacatgta aaaaagaagc ctaaagcaaa aattattaat acatgcacaa taggaacaga
    14881 tgcaacctta agtccaacat cagcgataca agcaatggat aaacttttag agatagaaag
    14941 tcttaattat ttagatgtat ctgctgttga atttaatgaa gcatttgcag ttatagatgt
    15001 gttgtttcaa agaaaatatc cagagttaat agatagatat aatatatttg gtggagcttt
    15061 agcgtatgga catccatatg gtgcttcagg tgcaattatt gcattacatc ttttaaaagc
    15121 attagaaaag actaagggta gatatggaat ttgctctata gctgcagctg gtggtctagg
    15181 ttcagcttta ctgcttgaaa gggtgtagat tatgaactat tatgaacttt taagaataaa
    15241 aaaagagagg cataaaaata aaaatttttt aattatagat ggagaaaaat atacttatga
    15301 aaatatattg agttatagtg aggaactagg aaaacaaatt tctttaggtg aaaatatccc
    15361 aattttaata tattctaaaa atataatgtt tcagttgatt agtttttttg ctattaatta
    15421 ttcaaaaaat attcctatta tttgtcatta taatctttct aaaaaagtat tagatgatat
    15481 tttattaaaa aataatattt caatcattat atctgatgaa tctatggacg ttattgattt
    15541 atatgatgat aacaataatg aagtagcaaa aaaagttaat ttttctattt ttaatcctaa
    15601 atttaatata tatatgtatc aatataagaa ccttataaat tatttagata aaagtatttg
    15661 tatgggagct ttatcttcag gttctacaag tgttccaaag gtattatata gaacatatga
    15721 gagttgggct ggattttttc ctatacaaaa tggtattttt aatatctctg gggaatcaat
    15781 cttgtttgtt aatggaacac ttagttttac agggaatcta aattctataa tgtcagtatt
    15841 atatgaagga ggtagtattg taataagttc aggtcttaat tgtaaatcat ggataaggat
    15901 aataaaacaa tataatgtaa ctaacatata ccttatacct acaaaacttc aacttttggt
    15961 taaacactta aaagaaccag tactcaaagt tgtaagtatt tttacagggt ctcaattatt
    16021 atttgaagat actgcaagaa atctaaaaaa atatatgcca aagtctgaaa ttattttata
    16081 ctatggggca agtgaattaa attatattac atatttatgc tatgatgaac taattgaaaa
    16141 acctctaagt gtaggcagac catttccagg aatagatata tatattagag aaggaaaaat
    16201 ttttgtgaat acagaatatg cagtatataa tgcaaccaaa ccttattctg tcaatgatat
    16261 tggatattat gataatgatg gatacttaat atttgaagga agaagtgatg acattttaaa
    16321 tattggagga tttaaagtta gttctactaa gattgaaaat gaagttaaaa aaattcctca
    16381 aattgaagat gcaattgtat tgccgtactc agacactata agaggaaatc aaattgtatt
    16441 atttgtaata actatagaca aaattacgaa gaaagatttg ttgattaaaa tgaaagataa
    16501 tctaatgaaa aatgaaatcc caaagaaaat tatcttttta aattcatttc catatacctc
    16561 ttctgaaaag atagatagat tagcattatt aaaaatgtta taagtataat atttagtaat
    16621 tttagtgaac caatgtattg ataaaaacat attgtatata gtaaaggagg cttacaatat
    16681 gtcaaatcaa tacgatataa ctgttattgg gggagacctg cgacaagtgt acatggtaaa
    16741 aaaactaatt aataaagggt attctgtagt cacttatggg atatcaaatg aaattataaa
    16801 tggaatcgtt ggaaaatcaa cttcactagc tgaagcacta tccaaaagtt ccattatttt
    16861 atgtccaatt ccatttacaa agaatcaagt taatattgaa aatacggatg attgtcttga
    16921 tgctactatt gataacttgc taattaattt atctcctaat caaatattat ttggaggtaa
    16981 cataccaact aaagtttgta aattttgcga gcaacactct attcagattt atgattttat
    17041 gaaaatgaat gatgttgcta ttttaaatgc agttgctaca gctgaagggg ctatagcaga
    17101 agctattaaa agaagtataa tcaatataca tcaaagtaac tgtttagtat taggttatgg
    17161 aagatgtgca caaatacttg caaaaaaatt atacgctttg gatgcaaatg tatgtgttgg
    17221 cgcaagaaat aatgatgcaa gaagtacggc taatgcattt ggatattcca gtatttcttt
    17281 aagtcaactt gatgaaaaac ttttacaatt tgattatatt tttaacacca taccagagtg
    17341 tattttaacg aaagaaaggt tagaaaaagt atcacaagaa gtaactatta tagatatagc
    17401 ttcttctcct ggaggagtag attattctgt tgttaaagaa gcagggatta atgcgactct
    17461 ttgccttggg cttcctggta aatattcacc aaagacatct ggtgaaattc ttgttaacgc
    17521 tatagaaaat attataaatg aaagaaggga ttagaaatga gacttgaagg acttacaatt
    17581 ggagtaggat ttactggttc gttttgtact tatgataaaa tatttataga gttagagaat
    17641 ttggttaaag aaggagctaa tgtacacact atattttcag atgtatcaca aaatatcgat
    17701 tgtagatttg gaaattctga agagtttatg aaaaaagcat atgaacttac aggaaataaa
    17761 cctatagtta cgattgaaga ggcagagcca tttggaccta aaggaattgc tgatattata
    17821 ataatagctc cttgtactgg aaatactgct gcaaaattag caaatggtat tacagattca
    17881 ccagtattaa tggcagcaaa aggtcatctt cgtaatgata aaccactagt aatatcaata
    17941 tcaacgaatg atgctctaag ctttaatttc aaaaatattg gcattctcct taattctaaa
    18001 aatatatatt ttgtaccttt tggtcaagat aattgcaaag caaagcctaa ttctatgatt
    18061 gcacatactg agctaataat tccaactata gaacttgctc tagagaacaa acagctacag
    18121 cctgttataa aaagtccaca tcaatagaat tggttcttat aatattatgt attagataca
    18181 atactagcaa agaggctgtc tcaaaataga gagaagcctc tttttcttgt tgaaatgatg
    18241 aaaatatatt taatttcata caaaaaaatg cctcatggac taaaaagtct attttgagct
    18301 aatctccgta atttttgcta tattaaaatt atttacttaa tccaattttt aaattagaat
    18361 ttatatcgtt ttgactcttc aaatatcaat ctaactgttg ttgatacaaa agaagaggac
    18421 ataattaaaa caaatactga tagtcctcta ataaacaaat tatctacata ataagttact
    18481 attccaatac ctccaatcgt taaaattaca cttataatca aagaaaaaac gctaattgtt
    18541 tttaattttt ccatgcttat cctccttctt ataaacttca aatttatcta tataatatta
    18601 aatatatttt atttcatatt atataaaaat catgaatata tattttttta attatactat
    18661 atgttgtaaa tgtttgtaca tttaactata tttactttaa cttgttaatt aagttttaat
    18721 tttcattttc aaggattttt atttttaaac ttgatagagt atcatattaa aaattctttt
    18781 tataaagttt tatttgcaaa aaatacaaaa atataaacgt taaaaaaata acgttatcct
    18841 taagttttta gatgatttca ttagtgattt gcgtaaaaaa taaagtgata tctagttaaa
    18901 ttaagtattt gtaaagctag atattattag tagaaatgat gttatattag ataaaaaata
    18961 aaatgttaaa aaaataactt gaaaatagga aaagtaaatg ctataatgtt aataaattaa
    19021 cataataaat aaaaaactat ctcaggaggt tgttaaaatg gctcataaag aacaagtaat
    19081 aatagaaaat aaaaaagaaa atgaagttat gtcagttggt ttagatactg taaataacgt
    19141 tgaaagctta gttaaaaaac tagcaaaaat cagagaagct caaaaaatat ttgctacata
    19201 tagtcaagag caggtagata aaattttttt agcagcatct ttggcagcta ataagcaaag
    19261 agttcctcta gctataatgg cacaaaaaga aactggtatg ggaattgctg aagataaggt
    19321 tataaagaat cattatgcct cagagtatat atataatgca tataaagaaa ctaaaacttg
    19381 tggtgttata gagaaagacg aagcatttgg tatgactaaa attgctgaac caataggtgt
    19441 tattgcagca gttgtaccta caactaatcc aacttctact gctatcttta aagcactttt
    19501 agctttaaaa actagaaatg gaataatttt ctctccacat ccaagagcaa aaaattctac
    19561 tatagaggct gcaaaagttg ttcttgaggc tgctgtttta gctggagctc ctgaaggtat
    19621 aataggatgg atagatgagc catctttaga attaactaca actgttatga aagaagtgga
    19681 tttgaccctt gctacaggag gtcctggaat ggttaaatca gcatattcat ctggaaaacc
    19741 agctattgga gttggtgcag gtaatactcc agcaataatt gatgatagtg cagatataaa
    19801 aactgctgta aactcaattc ttgtatctaa gacttttgac aatggtatga tatgtgcttc
    19861 tgaacaatcg gttatagtat tagaaaatat atacaatgaa gtcaaaaaag aatttaaaga
    19921 gcgtggtgct tatttattag ataaagatga aacagaaaaa ataagaaata taattttagt
    19981 aaatggagga ttaaattcta aaatagttgg gcaaacagct tgtactatag caaaacttgc
    20041 aggctttgaa gtcccagtag atactaaggt tttaataggt gaagtagaat cagttgaaat
    20101 agaagaagct tttgctcatg agaaactttc accagtactt gcaatgtaca aggcaagtga
    20161 ttttgatgat gcagttagaa aagcagaaaa attagtagaa gatggtggat ttgggcatac
    20221 atcttcatta tatattgatg atgtaaatca aagagaaaaa cttgataagt ttacttcagc
    20281 catgaaaact tgtagaattt taattaatac tccttcttct caaggtggta taggggactt
    20341 atataatttt aaactagctc cttctcttac tttaggatgt gggtcttggg gtggaaattc
    20401 agtatctgaa aatgtaggag ttaagcattt acttaatatt aaaacagtag ctgagaggag
    20461 agaaaatatg ctttggttta gagcaccaga aaaagtttat ttcaaaaaag gttgtttagg
    20521 agtagcatta aaagaactta aagatgtaat gaataagaag agagctttta ttgtaacaga
    20581 tacattcctt tacaataatg gatatactaa agcagttact gatttattag atgaaatgaa
    20641 tattaagtac actacattct ttgaagtaga accagaccca actttggaat gtgctaagat
    20701 aggcgctaaa gctatgagag aatttaatcc agatgtaata atatctatag gtggaggaag
    20761 tgcaatggat gcaggaaaga taatgtggac attgtatgag catccagatg tagatttcca
    20821 agacctagct atgagattta tggatataag aaaaagggta tatacattcc ctaaaatggg
    20881 tgaaaaagct aattttgtag ctataccaac atcagcaggt actggttcag aagtaacacc
    20941 atttgctgtt ataactgacc aagatacagg agtaaaatat ccattagcag attatgaact
    21001 gatgccaaat atggctatag ttgattctga tatgatgatg aatatgccaa aaagcttaac
    21061 atctgcttct ggtatagatg ctttaactca tgcattagaa gcatatgtat ctatgcttgc
    21121 aaccgattat acaaatggat tagctttaca agctattaag agtatatttg aatatcttcc
    21181 acgtgcatat gataatggtg caaaagaccc agaagctaga gaaaaaatgg caaatgcttc
    21241 tacaatggca ggtatggctt ttgcaaatgc atttttgggt gtttgtcact ctatggctca
    21301 taaattaggt gctttccacc atgtaccaca tggtgttgca aatgctcttt tgataacaga
    21361 agttatgaaa tttaattctt cagatgcacc aaagaaaatg ggagcattct ctcaatataa
    21421 atatccagaa gcattaaaga gatatgctga aatagcttca tttttaggat taaaaggaaa
    21481 ttctgatgaa gagaaattcc aaagtttatt ggtagctatt gaagatttaa aacttaaagt
    21541 tggtattcct aaatctataa aagagtttgg cgttgaagaa tctaagttta tggattcaat
    21601 agatgaaatg gtaattcaag catttgatga ccaatgtaca ggagcaaatc caagatatcc
    21661 acttatgaat gaaataaaag atatgtattt aaattcatat tatggaagat aagtttgcta
    21721 taaaatagat aaaataaaaa ctcttataca taaaacaaag ttttattgta taagagtttt
    21781 tttgtttgag aaaatggata gtttttattc tttaatttaa aattcaatta aatagtgtta
    21841 ttataataat aaaagaatgt ttttatggtt attatgttga ttttatgtgt aacatttaag
    21901 aatagttata tacaatataa atgatgaatt ggtataaaaa ggatatatta tctatatgaa
    21961 gtattgacta attaggggag gagtcgaatg acagggagaa ataaaagttt tacattaatt
    22021 acaattcttt ttttaacatc attatttttg acaatggggt atatctatat agaagataca
    22081 aaacatcttc ttatgtcaga ggcaaaaata catataaaag aagttgcagt acaaggctct
    22141 caactagcac aaagacaaat tgaaaaagat ttagatgttt taaatatttt ttctaactat
    22201 tatgcttcaa atccagatat acctaatgag gaaaaaatga aaaatctttt agatgaaatg
    22261 gaaaatcaaa aattttacac tatggcaata gttgatataa atggtaatgc tgaaaatacc
    22321 aaaggtgata agttttcagt gaaagataga gaatttttca aaaactcaat taaaggtaag
    22381 aagtatgttt caagtccata tgttgatgaa gtaaataagt ctataaagaa gattgctatt
    22441 tcagtaccat tattaaataa tgataaagta gttggagttt tatattgtac ttacaatatc
    22501 aatactctga tgaaacttat taatatatct ttttatgaaa ataatagtat atcttatgtt
    22561 gtaaaaaatg atggtacaat tatccttcat ccacaagggg atagcctatc aaaaaatatt
    22621 tataaattgt tagaacagga taatgatatt caagaagtaa atagattaaa aaaagaattg
    22681 caagaaaata aaacaggtgc aactgtatta aatatgttgg aagaaagaag atatttaggg
    22741 tatgccacta tggataatgg gaatagtgaa aataattata taaaagactg gaatttaatt
    22801 ttttcaattc cagaaactgt tgtatttagt aattcaaaac aaataatcaa cagagctgta
    22861 tatgcagtgt tatctattgt attaatattt gttgcaatta ttttttatat aatttatata
    22921 aaaaaatcta atgaaaaaga aatattaagt ttggcttatg aagataaagt tacatatata
    22981 ggaaatcaaa ataaatttta tagagaatgt agtaagtatc ttttagataa accatcttta
    23041 aattatatta tagtatattt tgatattaat aactttaaga tgattaatga tacttttgga
    23101 tatgaatttg gtgacaattt attaattaca atagcaaaag ctttaaaaga agaattaaca
    23161 gaaggtgagg tatatgcacg actttcaagt gattattttg caattttctg tgattataaa
    23221 aatggaagaa ataaaattat aagaaaactt gataatatac gaagtaatat tgaaagtaat
    23281 ttgagtatag tctttgaaat ttctctgtgt gtgggtattt attttgttga agaaggtgaa
    23341 gtagatattc aaaaggctgt aaataaagca aatatggcta ggtctgttgc taaaggtaaa
    23401 aatataaatt atgctattta taatgaagat gttaggaata agcttagtga ggaatcaatg
    23461 attttagacg atataaaaat agcattggta aaaaatcaat ttgaagtgta ttatcaacct
    23521 aagttttcac ttgtaactgg agaaatgata gggagtgaag cactaataag atggaatcat
    23581 ccagaacatg gttttataag tcccgcagta tttattccaa tagcagagaa aagtaagttg
    23641 attttaaaaa ttgggagatt tgtatttgaa agggtttgca atgatttata tgaatggaaa
    23701 aagcaaggta aaaagatagt tccagtttca gtaaatttat ctagggtcga gttgtatcaa
    23761 ccagatattg taaaatttat caataaaaca ataaaaatgt ataatttaag ttcagatttc
    23821 attgaaatag agataacaga aactgtggct attaatgagt taaatatttt aaagaatgtt
    23881 ttaaatgagt tgaggacgta tggattttct atttctatgg atgactttgg tacaggatat
    23941 tcttctatta gttgtttaag agatatgcca attgatatat tgaagctaga taagtctttt
    24001 cttggtggca ttgaacatga tgagagaagt cgcaatatag caaaatcaat agtgtctcta
    24061 gcaaaatcac tagatttagt tgtaattata gaaggtgttg agagtaaaga acaagctgag
    24121 ttaatgaaac aatttggatg tgatttagtt caaggatttt attttgcacg accaatgcca
    24181 gctaagaatt ttttagattt actttaaaac aaaatttgat ttttatataa agcactatta
    24241 tgaatgcttg attttaataa atcattgcta aataattagt gtttttttat tttatcaatg
    24301 tattgtctaa tataaagcaa aaaataattg tattttataa aaattatatt aaaaataaat
    24361 ttattttgct aataaacatg ttattaacaa aagaatagta ctataatgta tagatgataa
    24421 aaactttaat aaaaatttag tgtataggag ttgtttaagt atgatagata taagtattga
    24481 tgacaaactt aaggaaagat ggcctaatgc taagctgggt tgcattcaag caaaagtagc
    24541 agtaaataaa agctcagaaa aattaattaa tgaaataaat gagttttgtg atactataaa
    24601 tcaaagtcta aaagttgaag acattacgaa actagataaa attaaagatg caagaaatgc
    24661 ttataaagag cttggaaaaa atccaagtag atatagaaca tcttcagaag cattggttag
    24721 aagaattatt caagggaagg gtctttatac aataaataat atagtagaaa taaacaattt
    24781 aatatcaata aaatcattat atccagtagg tacatataat gtaagcaaat tacattcacc
    24841 agtatgtttt actgtaggag atgaaggaga acagtataag ggtattggga aagaattaat
    24901 aaatatagaa aatcttccta tattgtctga ttcagtgggt aaatttggga gtccaactag
    24961 tgattctgaa agagcaatga taacagaaga tgcaaatgaa atattaattt gtatattttc
    25021 ttttagtcaa gaatctgata ttgaagaata ttttggatat gctaaggaat tacttaaaga
    25081 atatgcagat ggaaaagata tagaaactaa aataattaaa tagaatctat ataacaacgt
    25141 tatgttaagc taaaattcaa tataaaaata agttaagata aagaaactaa cacaaaatta
    25201 gtttcttttt taaaatagta gtttagtgtt acaaaaatag atagatagtc ccacgattat
    25261 taaaagtatg gtaatatatt aatatagcag agattgtgtt taagggattt tgaaaggggg
    25321 gatttataat aatcatttta tctaaaagat aaaatggttt tatagaaata actatatatt
    25381 taaaaatata aataatgata cagtaatttg ggtatataaa ataaggcata catggttttg
    25441 tttaaaaacc aataaggtac ataggtaatt acaatgtaag gaaggattta attatgattg
    25501 atggaaaaga ggtaaaacaa gaaagtggac aaaaaaatct aaaatttaag atagctatag
    25561 tagtaggtgt tttgtttgta gcttatatag gaatggcaat cttttttaga aatcattttt
    25621 actttggaac gactattaat ggcgtaaagg cttcaggaaa aacagttgag caagtaaatg
    25681 atttgttagc atctagtact aattcctata ctttaaactt agaggaaaga aacaataaga
    25741 aagaacaaat aaaagcaaaa gatattgatt taaaatttac accagatgat aaagttcaaa
    25801 agataaaaga tagtcagagc gctattggat ggattggtgg aatatttaaa tctaaagagt
    25861 ataataatat gataactctt tcatataata aagatttact agaaaaaagg tttaatgctt
    25921 tatcttgttt tgatagtaaa aatgttgtag aacctaagag tgcttatcct aagtatgata
    25981 gcactaaaaa cgcttatgtt atagtaccag aggtacttgg aaacaaagtg aaaaaagatg
    26041 atttttataa gttagtacaa aactcaatta attctgggaa tacttctatt tctctggatg
    26101 aaagtaaagt ttatgaagca ccaaacaaca caaaggattc agaaaagttg aaacaagcta
    26161 ttaagacatt agataagtat gttggtgtag tcgttacata tgattttagt gaccgtaagg
    26221 aaactttaga tggttctgaa attaataaat ggttaaaagt agactcagaa aagaattatg
    26281 acataacttt tgataatgat gctatggtag aatatacaag aggtttaagt aaaaaataca
    26341 gtacttttgg tgactcaaga ccatttaaaa ctgccacagg taaagatatt acaatttcag
    26401 gtggaattta tggttggttg atagataaaa agaaagaagc tgaagcatta gttgatgttg
    26461 taaaagctgg taaaaatgtt acaagagaac ctatttacat acaaaaagct gtatctcgta
    26521 ataaagatga tattggaaat acatatgtag agattagtct ttctggccaa catatgtggt
    26581 tctttaaaaa tggagaaatg cttgtatcaa cagacgttgt aacaggtgat ttagctagag
    26641 gatttgctac accatcaggt atatatcctc ttaattataa ggctagaaat gtttcattaa
    26701 ctggtcaagg ttacagttca cctgtaagtt attggcttcc atttaatggg aacataggca
    26761 ttcatgatgc tacttggaga aattcttttg gtggaagtat atataagtct tcaggttcac
    26821 atggatgtgt aaatacacct ttttctaagg caaagaaaat atatgaaaat atagaaccag
    26881 gtacaccaat tattttatac tagtaaatgt gttgctaaca gttaagcgaa gtcatcttag
    26941 tttatttagg atggcttcct ttttatataa agcttattga agtttacaga aagactactt
    27001 aaaaggagaa atacataatg aatgaaaaat taactaaaat tttagaagaa gctaagaatg
    27061 atgatgaatt tagaaaggaa cttataaaaa caagagaaca acgtgatgtt atggattcat
    27121 tttgtaaggt tgttacatct agaggttatg atatgacagt aggggaactt ttttctatgg
    27181 gagaagaata tacatccaat ctactgaaaa gtgtaaatgg aggagcagca tatcctattg
    27241 aagattggag tgattggtac gaggattttt ttataatgtt gtcgaatata tagagtaact
    27301 aatgatatta tctacttaag attaatacat tgccttaaaa taaaactatt agaactaggt
    27361 gtaagtttta tcaatcttta aaaatattga tttatttcta actagatata aaaaaattag
    27421 attaaaaata aggtgacaaa aatcgatata gcttgatgat ttataaaagt tagataaaat
    27481 agtgcgagat atttctaaaa attcaaaaaa aacaataaac ttcaatggtt agaggttgaa
    27541 aaagaatata aaaaactttt atatgtaata aattaaatta aaattataaa aaaaataaaa
    27601 taaaaattta aaatgtcgac ataacgttaa ttttaaaaca aaataacgct attgacaaac
    27661 tatatcgctt taaattataa ttattgaaaa taatggtatt taaaaaccat actcatgtca
    27721 tagtctaaaa gtaagtcaaa aaaggatgta aatctaggca agtactattc taaaatatgt
    27781 tttattaaga aataaacgtt gaaaaatatt aattattata taaaaatgct gtttatactt
    27841 taacaatgag gtagtaatct aattagaata aaaaaatcta attggttata tacatagtaa
    27901 aaaggtaaga gaggtgattt ggatggcttt tgaaataata aaaagcattg ttgaggcaga
    27961 gcagacagca gacagtatca aagtaaaagc tgttactgat gcagagtcta tcagagctga
    28021 tgctgtaaac aaatgtgaaa gcatatttgc tgatgtaaaa aaacaagcaa agcttatgga
    28081 agaaactctt attgagaagg cagtcaccga cagtagagca gaggttgata aaatcttagc
    28141 taatgctaaa agtgaatgtc tgaaaattga aaaaactgct gaagaaagaa aaagtaaggc
    28201 tattgaagcg gttattggaa aggtagtgag ataatatggc aatagttaat atgaacaaaa
    28261 tatctattgt tggcttagaa gctcaaaaaa gccagatttt aaagctactt atgaaccgtg
    28321 ggtttgttca gattgatgat agtgcctttc ttactgagga ggaggaatta aaaaattatc
    28381 ttgtaaaaga tagtgaagaa tctgaagtta taatgctaga gcagaaaatg tctcatgtaa
    28441 accaagcaat taatattgtt tcttcactag tgaaacaaaa aaaatcccta cttgcaccaa
    28501 aaagagagtt taacaaaatt gatagtgaaa aagaagggca agtttatgct gaagctctgg
    28561 agttaaacaa tttgcacaag gagataggta caataaggtc taacataaac ttacttaaca
    28621 acaataaaag tatgttagaa ccatgggtga actttgatat tcctattgaa aatatggaaa
    28681 ctaagtatac aaaaactata cttggaactg tgcccataag cgcaaacatt gatgctttaa
    28741 gagtaaaatt agaagatgaa ataccagaaa gtgttattgg gattgtcaat tctgacaagt
    28801 taatgaacta tgtttattta attactttaa aagaaacttt tgatgatgtt ttagaagttt
    28861 taaaagaatt taatttttca gtggtttcat taccaaatga agaaggtaca ccgttacaag
    28921 ctatcaagaa atacaatgaa atgataatta gacaggaaaa cgtttctaag gacaaaacag
    28981 aggaaattaa aagacatgca gatagtatac acatgcttga aaacctatat gatttttaca
    29041 ctatagaaag agaccaaaaa aagattatag agagacttgt caaaacaaaa acaacattct
    29101 gtcttaatgg ttggttacca agcaaaaggg cagatgaatt aattaaagaa attacagaaa
    29161 agtttgattg ttgtgttcag actgaagaag gaagtaaaga agaaggcttc ccaattctgc
    29221 ttgaaaacaa taaattagta acaccttttg aaagtgtaac taacatgtac agctatccaa
    29281 gtacaaaaga tattgaccca aatacaattt taactttctt ctttgttata ttctttggaa
    29341 tgatgctaag tgacgccgca tatgggttaa taattgcagt agtttgtgga tttgttgtat
    29401 ataaattaaa gatacaaaaa ggtgaaggaa atttaattaa acttattggt atctgtggag
    29461 tttcaactac tgtatggggt cttatattcg ggggtatttt gggggattta attcctataa
    29521 aagcattaat taatccttta gaggatgtta tgacacttat gggaatgtcg ttactgttcg
    29581 gtattatcca tatatatgta ggtttgggaa taaaagcata tacattaata agaagtggaa
    29641 aggtaataga tgcaatcttt gacataggtt tttggtattt gtgtataaca ggtgtatgtc
    29701 tacttataat accttttgtt gcaggggaca taggtgtatt ttcagaagta ggaaaatatt
    29761 tagcaattat aggtgctata ggtcttgtac ttactcaagg aagaagcttt aaaggtattc
    29821 ctgttaagct ttttaaagga ttttcaagtc tctatggtat aacaagttat tttgctgata
    29881 tattatctta tacaagaatt atggcgcttt gtttaacaac aggagttata gcacaagtta
    29941 tcaacctttt aggggctata gctggtccga tacttgcagt tgtaattggt gttgtaggac
    30001 acacaataaa ccttcttata aatgcactag gtgcatatgt tcatacaagt aggttacagt
    30061 atgtagaatt tttcaataag ttttatgaag gcggaggagt tccttttgtt ccgtttaagt
    30121 ataaaacaaa atatacaagc attaataaaa aggagatgta aatatggaat ttttaaacgc
    30181 gttaggaaat ggtcaatttt tggcaatatc aggagctgct atagcagcat tatttgcagg
    30241 tatgggttct gctaaaggta cttcaatagc aggtcaagcc gcagcaggtg ttgtaacaga
    30301 agaccctagt aaattcggtc aacttctagt tttacaactt cttcctggta cacaaggtct
    30361 gtatggattt atcatagctt tccttatttt atcaaaagca gggattattg gtggagatgc
    30421 aataccaaca tcagctcaag gtttacaact tcttatggca ggacttccaa ttggtttagt
    30481 tggtttagta tcaggtatag cacaaggtaa agcagcagca gcaggagttg gaatagttgc
    30541 aaaaagacct gaagaacttg gtaaagctat aacatttgca gcaatagttg aaacatatgc
    30601 tcttttagga ctacttgttt ctttcttagc ttacaatgga attgcaattg gttaattata
    30661 aattataatt cataagtaaa aaaaatgaaa aggaggaagc actcctatgg gtaatgaaca
    30721 aaaaatgatt gacagaatta tagctgatgc caaacaagaa gcacaagaaa tattggataa
    30781 agctaaaagt gaagcagatt taaaagtaaa ttcagctaat gaaaaagctg aaaaggaaat
    30841 ggcttcctat acaaagcttg cagaggcaga agcagaaaaa gcggcttcta aggaaatatc
    30901 aggagcttat atggaggcta aaaagcaaat attatcaaaa aaacaagaga ttttagaaga
    30961 ggtaatttta gaagctaaaa acaaactttt aaatcttaaa gataatgaat atgaagagat
    31021 tatactaaac atgattgaaa aatcaaattg tacagatgat tcagagatag tattatctaa
    31081 aaaagataaa aaaacattaa aagatgtgct atcaaaaaaa ggcattaaag tgtctgatga
    31141 aactagagat attactggag gtttcatagt taaaaaaggt gacattgagt acaattattc
    31201 atttgaagcc ataattgcag tagagcatga atatattgaa caaattgcag cagaaatttt
    31261 gtttaattaa ggaggggtta aaaaatggca gaagaaaaaa cttatcccta tgccgttgca
    31321 cgaataagag tgcttgaaaa acagcttctt aacagacaaa tgtttattca aatggcagaa
    31381 gccaaaagcc ctgaagactc tttaagaatt atagctgagg caggttatgc tgatacatca
    31441 aataataata tacataattt tgagaatatt ttgactcaag aattatcaaa gacatatgaa
    31501 attcttaaaa gtctagctcc tgaagaaaaa tttgttgatg tatttcttta taaaaatgac
    31561 tatcacaatt taaaagtgct gattaaagaa gaaatttctg gtgtagatgg tgagaaatat
    31621 ttaattgatg gaggtacaat tcctcttatt aagcttagag aatctttagc aaatcgtagt
    31681 tttagtgatt tacctaagat aatgtcatca gctatattag aggcatttga tgtttataat
    31741 aagacacaaa atggccagat ggttgatatt gttctagata aagcaacctt taccagtatg
    31801 aaagaaactg ctaaagaaag ccaaaacaaa tttgtaatag attatgttat gaaagtttgt
    31861 gatttaacaa atctaaagag ttttataaga gtcaaaaata tgaagaaaga ttttgatatg
    31921 tttatgaatg tgtttgtttc aggtggctca cttgataagg aaaaattctt agaagctttt
    31981 tcctcagata ctccagcatc atgttttaaa tctacttctt actctgatgt ctgtaaaaat
    32041 ggcatggata gtggttttac tgtctttgaa aaactttgtg atgactatct tatggaatat
    32101 gttagaggag caaaatttaa gcctcttaca ttagaaccac taattgcata tttatatgct
    32161 aaagaatcag agataaaaac aattagaatt attttgacta gtaagcttaa taatatagac
    32221 gcagatacta taaaagaaag gctaagggat gcttatgtat aaggtaggag tagttggaga
    32281 taaagatagc atcatgggat ttttagctct tggaatagat atcttcccag cctatgatag
    32341 tgacgaaatt aaaaaaagta tacataagtt ggttgaagat gaatatgcga taatttatat
    32401 tacagaacag gcatcattgc ttgcaaaaga atcaatagca aaatacaaag attaccaact
    32461 tcctgccatt atagttattc caggtattgg aggaagtatg ggattaggta tgaatgaagt
    32521 aagagaatct agtaaacgtg ctataggtgc agatatatta tttaaagaat agatgaaagt
    32581 tgaataaaag acaatctcaa attttattgg tgctaaatat atgtgctaat aaaatatagg
    32641 agggaatatt taaattttga taaagtatat gattgcaggt gagagaatat gaaaacaggc
    32701 acaattgtaa aggtgtcagg accgcttgtt gtagcggagg gcatgagaga tgcgaatatg
    32761 tttgacgtcg taagagtttc agataaacat cttataggcg agattataga aatgcatgga
    32821 gataaagctt ctatacaggt ttatgaggaa acttccggtc taggaccagg ggaagaagta
    32881 gtttctcttg gaatgcctat gagtgttgaa cttggaccag gtcttatttc aactatttat
    32941 gatggtattc aacgtccact tgaaaaaatg tatgatattt caggtactaa tataactaaa
    33001 ggtgttgaag tatcttcact tgatagaaca actaagtggg aatttaaacc taaaaaatct
    33061 gttggtgata aggttgtagc aggagatata attggtggcg ttcaagaaac agcagtagta
    33121 gaatgtaaaa ttatggttcc ttatggagtt aagggtacta taaaagaaat atattctggt
    33181 gaatttactg ttgaagatac agtttgtgtt attacagatg aaaaaggtaa tgatatacct
    33241 gtaactatga tgcaaaaatg gccagttaga aaagaaagac catatagaga aaagaaaaca
    33301 ccagattcac ttcttgttac aggtcaaaga gttatagata catttttccc aatcacaaaa
    33361 ggtggggtag cagctatacc gggacctttt ggaagtggaa aaactgttac acagcatcag
    33421 cttgctaaat gggcagatgc agatatagtt gtatatatag gctgtggcga gcgtggaaat
    33481 gaaatgacag atgttcttct tgaatttcct gaattaaaag acccaagaac aggcgagtca
    33541 cttatgcaaa gaactgtgct tatagcaaac acatcagata tgccggttgc tgcacgtgaa
    33601 gcttctatat acactggtat tacaatagct gaatatttta gagatatggg atatagtgtt
    33661 gcacttatgg cagactctac atcaagatgg gctgaggctc ttagagagat gagtggtcgt
    33721 ttagaggaga tgcctggtga agaaggttat cctgcatact taggttcacg tcttgctcaa
    33781 ttctatgaga gagcaggaaa ggtaaattgt ctaggtatgg atgaaagaga aggaacactt
    33841 acagcaattg gtgcagtttc tcctcctggt ggtgatacat cagaacctgt cagccaagca
    33901 acgcttcgta tagtaaaagt attctggggt cttgatgcat ctttagcata taaacgtcat
    33961 ttcccagcaa ttaactggtt gactagttat tcactctatc aggaaaaagt tgatgtatgg
    34021 gcagataaaa atgtaaatga taatttctca gctcaaagag ctcaagctat gaaactttta
    34081 caagttgaat ctgaacttca agaaatagtt caattggttg gggttgattc actttcagat
    34141 ggagatagat tgatactaga ggttactcgt tctataagag aagacttcct tcatcaaaac
    34201 tctttccatg aagttgatac ttatacatca cttcataaac aatatagaat gatgggactt
    34261 atattaaaat tctataagtc agcacaagat gctatcaagc aaaatgttac tataaacgat
    34321 attatcaagc tttcagttct tgaaaagatt ggtcgtgcta agtatgtaga agaaggcgat
    34381 atagatgcag cttacgattc aattgaagat gaaatagaca acgagcttgc agaccttatt
    34441 aaaaagaggg gggctgaaga actatgataa aggaatataa gtcaatacaa gaggtagccg
    34501 gtcctcttat gttaataaac ggcgtagaag gtgtaaaatt tgatgaactt ggagaaattg
    34561 aactttcaaa tggagaaatt cgtcgttgca aagttctaga ggtaaatgga gatactgcac
    34621 ttgttcagct tttcgaaagc tcaacaggta taaaccttgc tgaaagtaaa gttcgttttt
    34681 taggaaagag tcttgacttt ggagtttcac cagatatatt aggtcgtgtt tttagtggaa
    34741 tgggtagacc tattgatgga ggtccagaaa ttataccaga taaaagactt gatataaatg
    34801 gagctccaat aaatccagca gcacgtgatt atccggccga gtttatacaa actggagtct
    34861 ctgctataga tggacttaat acattggtta gagggcaaaa acttcctata ttctcagggt
    34921 ctggtttgcc acatgccaat cttgcagttc agattgcaag acaggcaaaa gtacgtggaa
    34981 ctgattctaa gttcgccgtt gtatttgctg cagttggtat aacttttgaa gatgctgaat
    35041 tttttataag cgattttaaa gcaactggtg caatcgaccg ttctgtagtg tttgtaaacc
    35101 ttgcaaatga cccagctgta gagcgtgtat ctacacctag aatggcactt acagcagcag
    35161 aataccttgc tttcgagcaa gatatgcatg tacttgttat aataacggat attacaaact
    35221 atgcagaggc attgcgtgaa gtttcagcag ctaaaaaaga agttccagga cgtcgtggat
    35281 atccaggata tctttatact gaccttgcta ctatgtatga aagagcagga aaaatgaaag
    35341 ggcatgaagg gtctataact atgattccaa tacttacaat gcctgaggat gataagacac
    35401 atccaattcc tgaccttaca ggatacatta cagaagggca aattatactt agccgtgaac
    35461 tttataaaaa agatatacag cctcctatag atgttttacc atcactttct cgtctaaaag
    35521 ataaaggtat tggtaaaggt aagactcgtg aagaccatgc agatacaatg aaccagcttt
    35581 ttgcagctta ttcaagaggt aaggatgcaa aagaacttat ggctatactt ggtgaatctg
    35641 cactttctga gattgattta atgtatgcta agtttgcaga tgaatttgag aaagaatatg
    35701 tttcacaagg atttagaact gatagaacta ttgagcagac attagaaatc ggatggaagc
    35761 ttcttagtat gttaccaaaa agtgaactta aacgtatccg tgatgaatat tttgaaaaat
    35821 atatgcctaa ggagtgatat ctgtggctag attaaatata aatccaacac gtatggaaat
    35881 gactagactt aagaaactcc ttaaaactgc tacaagaggt cataagcttc ttaaagataa
    35941 acttgatgaa cttatgaaac aatttcttga gattgtaaga gaaaataagc gattgcgtga
    36001 agaagcagaa aatgctcttg atactgctta taaaaacttt ataatcgcaa gagcagttat
    36061 gagtcaggag tatctgggtt cagcacttat gatgccaaag caatcagttt cagtagatgt
    36121 ttctacgaga aatataatga gtgttgatgt tcctgtgttt gactttaaaa ctgaaaacaa
    36181 tcaaagtgat atttatcctt atggtcttgc ttttacatca ggtgaattag attctgctat
    36241 ggaagctttc tcagatgcta tgcaaccact tttaagactt gcagaaagtg aaaaaagtgc
    36301 acagcttctt gcacaagaga tagaaaagac acgtagacgt gttaatgctc ttgaaaatgt
    36361 aatgatacca aattacattg agactattaa gtatatagct atgaagcttg aagaaaatga
    36421 gagagcaagt actactagac ttatgaaggt taaggatatg gtgcttaaaa aagcacttga
    36481 agaaaagaaa aaaaatgatt tggttgtata attaaatact tataaaaata agtctaacat
    36541 tctactttat agaggttaga cttatttttt atgtttaaat taaaatgtag atactataaa
    36601 aattaatata ttgtttaaaa acagactgta tttaactaaa aataatatat ttaacaaaaa
    36661 tttctaaaaa ttaacgaact gttaatttac acttaacttt atattaacaa attaggtgta
    36721 ttatataaac ataagcaaaa acaataaaaa acaaataaaa aaaggaaaaa acacaaaggg
    36781 ggaagatatg aaagaattta tatttacttg tctgtgcatt gtagtgatta catttatgac
    36841 aagttgcata tcaacaacac cacaaactac tcaaggaaaa ggaaataata aacttacaat
    36901 atatgcagca ctagaggaag agcagatagc ttattatatg gaaggtttta gagaagagta
    36961 tcccaatata gatgtagaga tagttatgga ttctcatggt gtaatagctt caaaactatt
    37021 agcagaaaaa gataatccac aagcagatat tatatgggga ttatcagcaa tgaacatgat
    37081 agagctaaaa aaagatggtg cacttgaagc atataagcct aaaaattatg ataaagttga
    37141 taaaaaattt aaagatactg aagatgatgt atcttgggta ggaatgagtg tcccagagac
    37201 agcaatagta gttaattata aggaacttaa gaaactaggt atagatattc caaaaagtta
    37261 tgaagattta ataaaaccag aatataaagg acttataaca atgccaaatc cagcatcatc
    37321 tggaacgggg tatcttacag tatcagggct tattcaaatg atgggtgaaa aagatgctta
    37381 caattacatg gataaattac ataaaaatat agccacatat acacactcgg gttcaaaacc
    37441 tgcgaagtta gctggctctg gagagtatcc aataggtatt acacttggat atagagctac
    37501 acaacaatta tctagtggag aacctataga ggttgtattt ccaaaggaag gttcaggttg
    37561 ggacttagaa gcaaatgcct tagttaaaaa agataatata aaaaaagaag caaagctatt
    37621 tttagattgg gcaataagtg attcagctat ggatagatat gcacatgaag tagcagttac
    37681 ttccataaag acagataatc cagttccaaa aggttattct aaacaaccat ttgaacagct
    37741 tataaaagaa gttgatttaa actctgctgc caaaaacaga agtacagtac ttaaaacttg
    37801 ggaatcaaaa tacggttcaa aatctgaaag taagtaaagt ttaaattaat aatttagatg
    37861 aaagaatttt agaagaaaaa gtaggatata aaatttaaaa taaaaaaata actatataag
    37921 gagagataag atatgagtta cttaaaaata aataatgtgt ttaaaagtta tgaccaaaaa
    37981 aaggttttaa ataatattag tttggatatt gaagaagggg aatttctatg tttactcgga
    38041 cctagtggat gtggtaaaac tacattactt agaataatag caggtcttga agatgtaaat
    38101 agtggaacta taattctcca ggataaagat attacaaatt tagagccatc aaagagaggt
    38161 tttggtatag tatttcaatc atatgcatta tttccgaata tgactgctta taacaatata
    38221 gcttttccac taaaagagag aaaagtttca aaagaaaaaa tagataataa agttaaggaa
    38281 gtacttgaaa cagttggatt gactaatgaa gctcataaat accctaaagc attatcaggt
    38341 ggacaacaac agaggattgc aatagcaaga gcattagctc ttgaaccaaa gtttttactt
    38401 ttagatgaac caatgtctgc attagatgca aaggttagac acaagcttag aatggacatt
    38461 aaaagattac aaaaagaact taatataacg acaataatgg taacacatga ccaagaagaa
    38521 gctataacta tggcagataa aatagctatt ctaaatggag gagatataat gcaaataggg
    38581 actccagaag aaatatacca aaatcctcaa aacctattta cagctcaatt tataggagat
    38641 acaaactgtt ttgataatgg agattctata ttaactgtaa gaccagaata tgttcaaata
    38701 gagaaatcga caaaagaaaa ctaccaaggg ataatatcaa atatagagtt tagagggaat
    38761 ttattgagag ttgaaataaa agataaatta aatgaaaatt tcataataag tgatgtttct
    38821 ataaaagagt gggttaattt aaatttagta gaaggtgacc ttgtaaaaat aagtatagac
    38881 gaaaaatact atttaaagta tccaaaagtt aaaggagcat aagtgtatga ataataatgc
    38941 tataagttct aaaagtataa gtcttaaatc aaaaatgaat ttagatataa aaaaatgtat
    39001 gcttacagta ttagttatgt ctttagtaat atttttatta gcacctataa tatatctatt
    39061 tgtacaggct tttttagata aagatggagt ctttgtagga ttacaaaact ttcaagaata
    39121 cttggcttct gaatctctat taaattctct aaaaaataca gtatttgtat cagttatatc
    39181 aactgtaata tcactaacac ttgcattagt atatgcatat gcattaacta gaacaaagat
    39241 taaatttaaa ggttttttta aatcattagc tatgtttcct atgtttgctc caacaatgat
    39301 gcatgcaata ggtctagtat atttatttgg aaatcaaggt ttggtaacta atttacttgg
    39361 agtgcatgtt ccaatatatg gagcagtggg aataatattg gcacaaatta tatatgtatt
    39421 tcctcaatca tttcaaatac tttatatatc tctaagttca accgattata gactatatga
    39481 agctagtaat actatgggga taagcaagat aagagaattt ataagcataa caatcccaag
    39541 tataaaatac tctttaataa gttctgcatt cgtatcgttt acattatgtt ttacagattt
    39601 tggagctcca aaggtgatag gaggagagtt taatgtatta tcaatagatg tatataagca
    39661 aattataggg caacagaata tgccaatggg agcaactgta tcaatattac ttttaatacc
    39721 tgcaataata tcgtttatag taactaaaaa aattgaaaaa aatcaaaatt catatataac
    39781 ttcaaagtca attccatata aggttaaaac taataagtta agagatagta ttttaggagg
    39841 ggttgtagga attattgcat ttggtataat agctgtaatg ttaactgtaa tcttatcttc
    39901 attaatatca gtttggccat atgatttaag actgacactg gaacattttt ctatgaaaaa
    39961 cagtattgat gggattaaac catttataaa ttcaataaaa atgtcactat ttacagctat
    40021 tctagggacg agctttgttt tcttatttgc atatttgaat gaaaaaacag aaaaagctcc
    40081 atatttaaaa tcatttgggt attttttaag tacattgcct atggcattgc caggattagt
    40141 tttaggtata gcttatgtat tattttttaa taaattagag tttaatttta tggatacaat
    40201 atacattaag aatttgttta atggattata tggaacatta cttataatgg ttatatgtaa
    40261 tatagttcat ttcttttcag tagcatttat aacagctact actgctatta agaaagtaga
    40321 taaagaatta gatatggtag ctaaatcaat gggaataaac agcgcatcaa ttcttaagaa
    40381 cgtaactata ccactttgtt tgccagcagt tttagagaac tttatgtact ttttcctaaa
    40441 ctccatggtg acaatatcag cattagtttt tatatatact gcaagttcaa aagtagctgc
    40501 aatatcaatt gtacaactag atgataaggg gagtactgca caggcagcag cactagctgt
    40561 gttaatatta gtcacaaata tattagtgaa agtaatatat gaatttataa agaatcgttt
    40621 agaaaaaaga acaaacttta attaaataat ttatcttata aaaacacaaa tttaaaaaag
    40681 tctattcttg tgatttatta tttaaagaat agacttttta aatttattat tgatattgtt
    40741 tattaatttt ccttttgtta tcatacattt gagaatctgc acgacgatat gtatcaaata
    40801 aagaaacatc tactttttca tcaaaaatag catatccaat agacattgat aattttaatt
    40861 cattagttga gttttcttca actattttta aattaatatt atttataata ttattt
//
LOCUS       DAEMGG010000023        40197 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEMGG010000023 DAEMGG010000000
VERSION     DAEMGG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.023"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            37..774
                     /gene="cas6"
                     /locus_tag="KPK24_001167"
     CDS             37..774
                     /gene="cas6"
                     /locus_tag="KPK24_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG2298872.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            779..2254
                     /locus_tag="KPK24_001168"
     CDS             779..2254
                     /locus_tag="KPK24_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298873.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFCELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMEKNVKRIVDIVIKSN
                     IEIDEIDKLDLKQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRIDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     MNTRNLGKKMINNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGREEYKMKNYIESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2274..3290
                     /gene="cas7i"
                     /locus_tag="KPK24_001169"
     CDS             2274..3290
                     /gene="cas7i"
                     /locus_tag="KPK24_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG2298874.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYRDKVHA
                     KTSIATITYDLRRILHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKEKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEREENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEEDGKLLLPEERKFMSKELREK
                     AVKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGV
                     IETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMW
                     YLKE"
     gene            3306..4103
                     /gene="cas5"
                     /locus_tag="KPK24_001170"
     CDS             3306..4103
                     /gene="cas5"
                     /locus_tag="KPK24_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG2298875.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4130..6538
                     /locus_tag="KPK24_001171"
     CDS             4130..6538
                     /locus_tag="KPK24_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG2298876.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            6980..7927
                     /gene="prmA"
                     /locus_tag="KPK24_001172"
     CDS             6980..7927
                     /gene="prmA"
                     /locus_tag="KPK24_001172"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG2298877.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKRKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            7946..8704
                     /locus_tag="KPK24_001173"
     CDS             7946..8704
                     /locus_tag="KPK24_001173"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG2298878.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8706..10004
                     /gene="mtaB"
                     /locus_tag="KPK24_001174"
     CDS             8706..10004
                     /gene="mtaB"
                     /locus_tag="KPK24_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG2298879.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10047..10397
                     /locus_tag="KPK24_001175"
     CDS             10047..10397
                     /locus_tag="KPK24_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG2298880.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10560..10739
                     /locus_tag="KPK24_001176"
     CDS             10560..10739
                     /locus_tag="KPK24_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG2298881.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10769..11212
                     /locus_tag="KPK24_001177"
     CDS             10769..11212
                     /locus_tag="KPK24_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG2298882.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11323..11820
                     /locus_tag="KPK24_001178"
     CDS             11323..11820
                     /locus_tag="KPK24_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG2298883.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12013..12576
                     /gene="raiA"
                     /locus_tag="KPK24_001179"
     CDS             12013..12576
                     /gene="raiA"
                     /locus_tag="KPK24_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG2298884.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12789..13025
                     /locus_tag="KPK24_001180"
     CDS             12789..13025
                     /locus_tag="KPK24_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG2298885.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13038..14381
                     /gene="yqfD"
                     /locus_tag="KPK24_001181"
     CDS             13038..14381
                     /gene="yqfD"
                     /locus_tag="KPK24_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG2298886.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14320..15435
                     /locus_tag="KPK24_001182"
     CDS             14320..15435
                     /locus_tag="KPK24_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG2298887.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15492..15953
                     /gene="ybeY"
                     /locus_tag="KPK24_001183"
     CDS             15492..15953
                     /gene="ybeY"
                     /locus_tag="KPK24_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG2298888.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            15959..16678
                     /locus_tag="KPK24_001184"
     CDS             15959..16678
                     /locus_tag="KPK24_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG2298889.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16797..17198
                     /locus_tag="KPK24_001185"
     CDS             16797..17198
                     /locus_tag="KPK24_001185"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG2298890.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17262..18155
                     /gene="era"
                     /locus_tag="KPK24_001186"
     CDS             17262..18155
                     /gene="era"
                     /locus_tag="KPK24_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG2298891.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18167..19546
                     /gene="mgtE"
                     /locus_tag="KPK24_001187"
     CDS             18167..19546
                     /gene="mgtE"
                     /locus_tag="KPK24_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG2298892.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19619..19774
                     /locus_tag="KPK24_001188"
     CDS             19619..19774
                     /locus_tag="KPK24_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG2298893.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19740..20513
                     /gene="recO"
                     /locus_tag="KPK24_001189"
     CDS             19740..20513
                     /gene="recO"
                     /locus_tag="KPK24_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG2298894.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20506..21105
                     /locus_tag="KPK24_001190"
     CDS             20506..21105
                     /locus_tag="KPK24_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG2298895.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21265..22143
                     /gene="glyQ"
                     /locus_tag="KPK24_001191"
     CDS             21265..22143
                     /gene="glyQ"
                     /locus_tag="KPK24_001191"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG2298896.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22143..24209
                     /locus_tag="KPK24_001192"
     CDS             22143..24209
                     /locus_tag="KPK24_001192"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG2298897.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKILIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(24375..25922)
                     /locus_tag="KPK24_001193"
     CDS             complement(24375..25922)
                     /locus_tag="KPK24_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG2298898.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            26202..26969
                     /locus_tag="KPK24_001194"
     CDS             26202..26969
                     /locus_tag="KPK24_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG2298899.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            27252..27449
                     /locus_tag="KPK24_001195"
     CDS             27252..27449
                     /locus_tag="KPK24_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2298900.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            27478..28554
                     /gene="vorB"
                     /locus_tag="KPK24_001196"
     CDS             27478..28554
                     /gene="vorB"
                     /locus_tag="KPK24_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG2298901.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            28557..29276
                     /locus_tag="KPK24_001197"
     CDS             28557..29276
                     /locus_tag="KPK24_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG2298902.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            29289..29828
                     /locus_tag="KPK24_001198"
     CDS             29289..29828
                     /locus_tag="KPK24_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG2298903.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            29985..31043
                     /gene="buk"
                     /locus_tag="KPK24_001199"
     CDS             29985..31043
                     /gene="buk"
                     /locus_tag="KPK24_001199"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG2298904.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            31061..31954
                     /locus_tag="KPK24_001200"
     CDS             31061..31954
                     /locus_tag="KPK24_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG2298905.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            31984..33192
                     /locus_tag="KPK24_001201"
     CDS             31984..33192
                     /locus_tag="KPK24_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2298906.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            33216..34475
                     /locus_tag="KPK24_001202"
     CDS             33216..34475
                     /locus_tag="KPK24_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2298907.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            35120..36583
                     /locus_tag="KPK24_001203"
     CDS             35120..36583
                     /locus_tag="KPK24_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG2298908.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            36639..37775
                     /locus_tag="KPK24_001204"
     CDS             36639..37775
                     /locus_tag="KPK24_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2298909.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            38011..39126
                     /locus_tag="KPK24_001205"
     CDS             38011..39126
                     /locus_tag="KPK24_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG2298910.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            39278..40147
                     /locus_tag="KPK24_001206"
     CDS             39278..40147
                     /locus_tag="KPK24_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2298911.1"
                     /translation="MDYLKYFKKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT        16126 a         4081 c         7448 g        12542 t
ORIGIN      
        1 aataatgaat tatactagaa agtaggaggt gaaaatatga gcagaatttc aatttttatg
       61 gaatcatgta atggcaaatt tccaaatgaa aattcaatgt tgtcagtttc ctttataaaa
      121 aatattttaa atgcagaaaa caaagatttt gctaaaaaca tatttaacta tggggaagaa
      181 agaaaaaaca ataagcaaat aaaggatata aatacagcca tttacatacc aaatcttata
      241 agaagcaaaa atgagttact tgttgatgga gatataatat tcaatataag tttttataat
      301 tattctatgt tcagtaaatt atacaatgga attttaaaag taaaggagct agaatataaa
      361 ggttatagat ttaaaataaa aaatataaaa atacataaag aacacgagat aaaagaaaat
      421 ggtgttatat ttaaaactat gtctcctata atagtaaaga ataaagaagg taaatattta
      481 gatgtagaag attcaaatta tatagaatgt ttaaactata tagctaatct aactctggag
      541 agtataagag gtagtgggct tagaaagcca ttggaattta tacctcttaa cttcaaaaaa
      601 agagtgttaa aagaaaagat tcgtggattt aaagagagag aattttacta tataaatgct
      661 tatgcaggaa catttttttt aaaaggaaat atggaagatt taaatgcact gtataaaatg
      721 ggaattggat atagaaggac ggaaaatgca ggaatggttg atatattaaa gtagcgaggt
      781 gagagaaata aaagctaaat tttatagatc aggtgatatt gtaagagata ttggcattgt
      841 ttacttttgt gaattactaa tggatttaaa aaacgaacta gaaaaaaatg attataagct
      901 aaaatttact tgtgagttga atagaaatta tttatcttta gaatctgcag aatatataga
      961 ccctaagtat atatctgatt atatattaga aaatcaactt tttaaggtat ttaaaaatgg
     1021 gaaaaaggaa accttagaaa aaatatttag taatatagat aatttaacat atgtaaattt
     1081 tattgaagaa ttggataaat cttctgcaac tgagaaacaa aaggaaggga ttaaaaaaga
     1141 ttttaaaaac agaagatttc catatgttag aaatagtggt aagtatggat taaacacgag
     1201 tctagaaaat atggaaaaaa atgtaaaaag aatagtcgat attgtgatta aatcaaatat
     1261 agagatagat gaaatagata aacttgattt gaaacaatat gaagaaaaag atagtgtatg
     1321 taatgtttgc aatataaata aaacaacaaa actagatatt gatttaagag aacgaaagga
     1381 ctctaagtat aactttttat ttagaggtgc agaaaaatca ggttttaaaa gaagtggaca
     1441 agttgaaagt aatatttgtt ttgagtgtga atttttcaac ttaatgtgtc ttttatatat
     1501 aaatttaaaa agaccaatgg tttttgcata tacgaatgat ttaagagaat tagcattttt
     1561 aaatcataag ataatgctta aaagaaaaat gtattcagat aaatcatttt ataaaaaact
     1621 tttgcatgaa aaaataagtt caattagact atataggatt gatattgata cgaataaagg
     1681 tataattcta aaatttgact ctattataga gtataaagaa ttattaaaaa aaattgaact
     1741 tatagatatt atagataatt ataatttttc aagagagcca atgaatacta gaaatttggg
     1801 taaaaaaatg attaataatg ggaatttatc aaatttaaaa gaattattac ttgataatat
     1861 aagtgtttta aggcaactaa ctggaacttc tcgagaaatg gattttgtat ctagttcata
     1921 taatatcggt ttatatataa aattgtgttg gattgtagat ggaagggagg agtataaaat
     1981 gaaaaactat atagaaagta atttaatata ttcaagagta ggtaaagact tatttaataa
     2041 aatgacagat gaatccagaa gaaaaaattt ttctatgaaa gtgattcaac tacttaagtc
     2101 taatgataga actcagctat ttcaaactat aatgcatgtg ttagtttcta atggaatctt
     2161 aataggagaa gggtttgtag aaggtctaat gcagagttct gaattagaat taaatacgaa
     2221 tgtaggtcta tttattcaag aaatcatgaa ataaaaaagg gggaaaattt aaaatgaatg
     2281 aaaaaagagt aaaaggagta acattaacta tactgacaga aagcccagtt ccactttcgt
     2341 atgaccaagg gtatggaaat tatacaccaa taaaaaaaga acaatataga gataaagtac
     2401 atgcaaaaac tagtatagct acaataacat atgatttaag aagaatactc catcaagaat
     2461 atggttggaa tttaagtaat attgtttttg gaaaaaaagg aaatatatat ccatctatta
     2521 agaaaaatgt aggatgtgta gatgaaaatg gattagagac tgatgtattt ggttatttaa
     2581 taccacttaa agagaaaggt tctataagta aggcttctcc gcttagaata atacctttta
     2641 ggtcactaaa ttcatataga ggttcaacac agttgataac aaatagaggt tttttaagta
     2701 gcgaatttgg tagaaaatat tatgatgaga gggaagaaaa tgaagtactt agagatgaaa
     2761 actttccaac aacacaagca ttagcaatag aagagacttt atcagattac tacgtttata
     2821 caataacatt ggagcttgat agaataggag tagtagaagt agaagaagat ggaaagttac
     2881 ttttaccaga agaaagaaaa tttatgagta aggagttaag agaaaaagca gtaaaagata
     2941 tattagatgc aattactgtg tttacaagaa acataaaaca ttcatcagta ctattaaaac
     3001 cacttgcagt aatgggtgga gcttttgata aagttgtacc atttttttgg gatgatgtag
     3061 attataatgc tgatagtggt gagataaatt tagagggagt catagaaact atagaaagtt
     3121 atagccttaa agaaagtaac acaatacttg ctatcaatga cagattaaaa atttctaata
     3181 aaaaggaatt aaataagttt aatttaagta aataccctgt aaaagagatt aaaaatctag
     3241 cagatagatt ggaaattgga gaagataata tgtggtattt aaaagagtag gaggtttttt
     3301 gaattatgaa agtattacaa tgtaaactta agcaaccaac tgctcactat agagacccaa
     3361 aagtatttca aaatgaatat ataagtactt taaatttacc atctaagacc acaataatgg
     3421 gaatgataac gtatttatgt gatagaagat taaactctga tatagatata ggaataattg
     3481 gaacacatca tcatagagaa ctagaatttt caagaggaga gaacattgat ttttggaatg
     3541 aatatacaaa tatgagaaaa ggcaaagata aagaaaagtt tttgcttcaa ggaaactact
     3601 atgattacta taaagaacat aaagctcaaa atagcatatt aaactatgaa gttcttaaag
     3661 aagtagaact gactatattt atttcttgca aggatgatga agaacttgaa tttattagaa
     3721 aaaagcttga aagtccatgt aaatatgcaa atttaggaag aaaagaagat tttgtaatac
     3781 caagtgaaaa agggtgtttt gtgaaagaag ttgagttaaa agaggttata cctatgaata
     3841 ctcgtgatgc tataaaagaa aatataaaat taaagaatac atatgtaaga gttgacttaa
     3901 gagataaaga gaatgtcgag actataataa atcaaggtgt attgattgca ttaccttata
     3961 aatataaaga tttagaagca aatagagatg atagagttta tgagttttgc cattatatat
     4021 atgttgataa tgatggtata tatcctaaaa atattaaggt aaatgtatgt attgatacaa
     4081 aagaagtttt tacatggctt taaattattt tgaaagatag gtgtttatca tgaaaaatat
     4141 catgcaaaaa gaaatattag ctaagtcata taatttaaga actaatcaac aagagcaaac
     4201 attagttgaa catattaagg atttatttga agtattagaa tctatattag aactaaatct
     4261 atattcagat aaagatgtag agatactaaa aatatgttgt gctttgcacg atttaggaaa
     4321 aattaattca attatgcaac aaaaaatgga gattaataat aaaatatcgt atagttgctc
     4381 agaagaagag aggaaaaagt tagagtctga caaaaaatct ttaaaaaaga tagcaagaca
     4441 taatatattt tctggagcat ttttaaaaga tatattagaa aaaatgaact tgtcagaaga
     4501 agataagtta tatatatata aaagtataat gttacatcat ggaaactatg aagattatat
     4561 gaggttaagt attagtaaag ttcaagaaga aatttatgag tatattgaaa aaggcatttt
     4621 agaaaaagaa gagtttaatc tgaaagatat agaaagttat ataaatagca ttttaaatgt
     4681 agactttaaa tttggtgaag atgtattgga ttatgattac atagataaat taagtgaaag
     4741 tatgtttata gacagtgatt ataacaatgg tcaaatagat gattcagttt taaattatag
     4801 aaagtttaag tatattttat ataaagggat gctgaactta atagaccata gtgcatcaag
     4861 tagacagaaa ggtataaaat tttataacga ttttactgat gaagaaattg ataatatgat
     4921 tttaaatgag atatataaga gtcagggtaa tttggaaaaa aataatatag agttcaatac
     4981 tatacaaaaa aggttgagag aactttctgg aagaaatgtt ctgactgttg catttacagg
     5041 ttcaggaaaa acagctgcag attatagaaa gacttttaaa agaaaattct ttttagtgcc
     5101 aaataaaata tcagcagaaa gtttttatag aaaaaatatt tttcaaaata aaaatgattt
     5161 agatacaaga agtagtaatg attacattgg attacttcat ggagatataa acttatattc
     5221 agagaatgaa gataatggag aacatgattt tgtattgaca ttaagggata tagatttgag
     5281 tataaacttt tgtaaacctt gtgtaattgc aacagttgac cagttattac ttagtatgtt
     5341 taaatttccg ggatatgaaa aaatattcgc agccgtaaaa aatgcatcta taactgttga
     5401 tgaagtacat ttattggaac caaagatgtt tttgattttg atatatttta tgcagtttgc
     5461 atgcaagtac ttagatgttg attttcactt aatgacagct acacttccaa aatcatataa
     5521 agagcaaatg ataaacaagg gaattatttt tcaagaagaa agtaatgaaa atgttactga
     5581 taaaggagaa atagttttta ttgagagcaa caaggaagaa gagatttgtg aaggtaaaga
     5641 tgtaaaggtt tcttttataa aggaaaaaga aatcaagtca atagttgaag atgctttaga
     5701 aaataaacaa aaaattctta taataaagaa tactattgat tcagtcaata gaacatatga
     5761 gtttttaaaa gaaaatttat ctgataagta tggtggtgta gatatagatg ttttacatag
     5821 tagatttaag ttcaaagata aaaaagagaa atatagtaaa atattaaatg gagagggtga
     5881 tatttggata tcaactcaat cagtagaaat atccttggat ttggatttca atataataat
     5941 aagtgattta gcaactatgg atagcttgat acaaagaatg ggtagatgta atagaaataa
     6001 taaatatgaa tatggaaact tttatattct acctagtgaa gataaaattt atgataacaa
     6061 acttaagaac acaactaaaa acatcttaaa ggacatatta aaaactaact ctatttttac
     6121 aatgggaatt agaaaaacta ttttggatga ttactatgat aatagtgtag tcaaaaaata
     6181 ttttgaagag aattttataa gttgtgataa agaaattaag aatatttatg gtataaataa
     6241 agaaattttt gatggattgg atttaatttt taattttgaa ccttacaaaa atattgttga
     6301 tagtaagaag gaagcagcta aaatttttag agatgttgat gttagttaca aaattattct
     6361 ggaagaagat ttttataagg aagatagaga gttacaacaa gattcgattc aagtatcagg
     6421 gtttatattt aatagattat attattatgg gttaataagc aaagtagaag gatacatggt
     6481 actaaaatct agtagtaagt ttgagtataa tagtactgta ggcttaattt tgaagtgagc
     6541 atatgtttaa atataatata cctttagaca ttgaaaatac aaaaagtact actgtaagct
     6601 cactgtaaaa actatttttt taaaaggaaa aataagttgt gagatagagt gtttacaagt
     6661 gtatatagat aataaatttg aggtttaaga ttaactaagt gggtttcagg tacatcgcga
     6721 aaataattat aagttggaga ttttgtatgt ttaagattaa ctaagtggat ttcaaatatt
     6781 gatatagtag atataataga agttttatta ttaggtttaa aattaactaa tcggattttt
     6841 aaatattctg actattatat aaggatatta ttagcttgaa tttaaaacca ataatataaa
     6901 acaacaaaat attatattta tgatataata aaagttatgg aaaaacagga atattactat
     6961 agagaagaga ggtaaactta tgaataattg gatagaagta actataaaaa caacaacaga
     7021 agcagttgaa cctataacga atatactata tgaacaagga gctggaggag ctgtaataga
     7081 agacccaaaa gactttttat tccaaaagaa aaatgaacta gactgggatt atgttgaaga
     7141 agaagtattt aaaaagaatg aagaagatga tgttcttata aaaacttatg tttctgaaga
     7201 aaaaaatgtg atggaatttg tcgaaataat aaagcgaaaa gttttagggt taaaagattt
     7261 tggtatagat ataggtgaag gaagtgtatc attggaccaa gtaaatgaag ctgattgggc
     7321 aaatgcatgg aaagcatact acaaaccaac aaaagtagga caaagggtag tagtaaagcc
     7381 tacatgggaa gattatgcta tgcaagaggg ggatttaatc atagagcttg accctggaat
     7441 ggcatttggt acaggtacac atgagactac tagtatgtgc attagagaat tagaaaagta
     7501 cgtgaataaa gattctaaag tatttgatat tggctgtggt agtggaatcc ttgcaatagc
     7561 agcagctaaa ttaggagcaa aagaagttgt agctgtagat ttagatgaag tggctgtaaa
     7621 agttgcaaaa gaaaatgttc ttgaaaataa agttgaaaaa agtgtatctg taatgcatgg
     7681 taatttaact gatgtaataa aagataaagc ggatgtaata gttgcaaata taatagctga
     7741 tataataaag atacttgcaa aagatgttca aaactttatg aaagaagatg ctatattcat
     7801 atcttcaggt ataattttgg ataaagtaga agaagttaaa gaaagcttaa ttgaaaatgg
     7861 atttgaaata gttgaagttc aaaaattagg tgaatggtca gcaatagttt ctaagcttaa
     7921 aaaataaggg atatggtgac gtataatgga tagatttttt gttgaaaaaa ataatataaa
     7981 tctacaagat aaaacttgca ctatagaagg tgaagatgta aaacatattt caaaagtttt
     8041 aagatgcaaa ttaggtgaga aacttgaaat atgtgacaag aataataatg agtatatttg
     8101 tgagataatg aatatagata agagtatagt taatttagaa attttagaaa aagttgatat
     8161 aaatagagaa tcagaactta aagtaaggct ataccaggga cttccaaaag ctccaaaaat
     8221 ggaaatgata cttcaaaaat taactgaagt gggtgtagaa gaaataatat tagttcaaac
     8281 taagagaagt gttgttaaag ttgatgacaa aaaagaagat aaaaaatttg aacgttggga
     8341 aagaataatt tatgaagcag caaagcaaag caaaagaggt aagataccaa aattaagagg
     8401 tgttttaagt tttaaagaag ctttagaaga tatgaaaaaa aataatgtaa atatttgccc
     8461 ttatgaaaat gaaagaacag tttcaataaa acatgcacta aaaaaatgtg attctaatat
     8521 agatagtgtt ggaattttta taggtccaga aggtggattt tctgaagaag aaatagaaca
     8581 aatacaaaaa aataattgta atgttgtatc attggggcct agaatattaa gaactgagac
     8641 agcttctgtt gtagcatcaa caatagcttt gtatgaacta agtgatttag gaggagaaaa
     8701 ataaattgaa aaaagtagct ttttatacac ttgggtgtaa agttaatcaa tatgaaacag
     8761 aagctatgct agaactattt gagaaagatg gttatgaaca agtcaatagt gaagagtatg
     8821 ctgatgttta tgttataaat acatgtacag ttactcatat gagtgataga aaatctcgtc
     8881 aatatataag acgagttaaa aagaagaatc cagatgcaat aatagccgtt gtaggatgtt
     8941 attctcaggt atctccagaa gaaattttgg atatagaaga agtaaattta gtcatgggca
     9001 caaatgatag aagaaagatt gttgaagaaa ttaaaaagat aaattcaagt aagaaagtta
     9061 gtacagttga tgacataatg aaagttaaag cttttgaaga aatagaaatc agccaaacta
     9121 atggaaagac tagagcattt atgaaaatac aagatggatg tgacagattt tgtacttatt
     9181 gtatcattcc ttatgcccga ggacgcgtta gaagtagaga tattgatagt atagtcgatg
     9241 aagtgaaaaa attggctaat aatggctata aagaggttgt attaacagga atacatgttg
     9301 cctcttatgg aaaagattta aaggataggg atattaaact tcttgatgta ataaaacaaa
     9361 taaaccagat agaaaaaata gaaagaatta ggcttagttc tgttgaacca attttattta
     9421 cagatgaatt tgtaaatgaa gttttaaaga tggataaagt ttgtcctcat taccatttat
     9481 cacttcaaag tggatgtgat gaaactctaa aaagaatgaa tagacgatac acaactttag
     9541 agtacaaaac tattgtagat agattgagaa gtaaaatgcc agatgtggca ataactacag
     9601 atgtaatagt aggttttcct ggagagacaa atgaagagtt taaaaaaacg tacgagtttt
     9661 taaaagaaat agagctttca caaatgcata tttttaaata ttcaccaaga aaaggaactc
     9721 cagctgcaac tatggaaaat caagttgacc ctcaaatgaa acactttaga agtgaacaac
     9781 ttttaaattt aagtaaagta aattttaata aatttgcaac taaatttatc ggccgtgagt
     9841 tggatgtttt atttgaacaa aatatagaag gcaataaata tgagggatta acttcaaatt
     9901 atataagagt tgttgttgaa agtgacaaaa atatacaagg tcagatatta aaagtgaaaa
     9961 taaatgatgt aaaagatgaa tatgtagaag gaattttgct ataacatgta gaaattataa
    10021 tatttataag ataggaggtg gaaagaatgg attgtatttt ttgtaaaata gcgaacggag
    10081 aaataccttc aactaaagtt tatgaagatg atagagtctt agcttttaat gacttgaacc
    10141 cagttgctcc atatcatata ttagttgtac ctaaaaaaca ttatgatagt ttaattgata
    10201 ttcctgataa agagatggac attgtgtcac atattcatgt tgttattaat aaaatagcta
    10261 aagagaaagg ttttgaccaa actggattca gagtcataaa taattgtgga agtgatggag
    10321 ggcaagaagt taaacatttg cactatcata ttttagctgg taaaaagctt cctaattacg
    10381 aagcgggaca aaactaaact aaattattga aaaattgagt tccttcttga taaaaatact
    10441 tgataaaagt aaaaatttag tttataataa acaaagtgtt aggttgtaat gcatattatg
    10501 caaaaagaac atagttacag tcccagcata attgcgctat tggagggagg gaaaaagaaa
    10561 tgtcagaagt aagagttaga gagaatgaaa cattagacag cgctttaaga agattcaagc
    10621 gtcaatgtgc aatgtctggc atcatgtcag aagtgagaaa aagagagcac tatgataagc
    10681 caagcgttaa gcgtaagaaa aaagctgaag cagcaagaag aaaaaacgct aagaaatagt
    10741 agtaaataag aataaagagg tgaaggggat gtcccttaag caaaagttac aagaggattt
    10801 gaaatcttcg atgaaaaaca aagatacagt gagaaaatct gtagtaactt taataagagc
    10861 ttctataaag cagtatgagg ttgataatag ggtcgagctt gatgaagatg gaattatcga
    10921 tgtaatagca aaacaattaa agcaacgtag agatgcatta gttgaatttg aaaaagctgg
    10981 aagagaagac ttaataaaag aaacagaagg tgaaattgaa gttttaaaag agtacctacc
    11041 tcaacaatta agcgaagaag agttagaaga aattgtaaaa tctactatat ctgaagtagg
    11101 ggcaacttct atgaaggata tgggaaaaat aatgtcagtt atccaaccaa aagttaaagg
    11161 aagagctgac ggaaaactta taaataagtt ggtaaaacaa aacttacaat agattgatat
    11221 agcctagaga gaaatctcta ggctttttat agtttttggg aataacgagt catatttgag
    11281 catatctata atcaaagaaa attttgaaag aaggggaaaa atttgattat atcagtaaaa
    11341 gagattagga attttttgat aaacatattt ataccactaa taataggtta cttaagtagc
    11401 atcttaacaa tgattatcag tggaacagat atttcaacct attatttaca actcacaaag
    11461 ccaagctttg caccaccagc ttttatcttt cctatagttt ggactatatt gtacatattg
    11521 atgggtatat cttcatacct aatattaaga aagggttaca acttacctaa agtaagagat
    11581 gcaatttttt attatggatt acaacttgta ttaaacttta tttggagtat actatttttt
    11641 ggatttggac ttagatttac agctttgata gatataataa taatgatttt ggtttctata
    11701 ataatgattt cgaagttttc taaaatagat aagagatctg gatacataaa tttgatttat
    11761 ttaatgtggc ttgtttatgc aggatttttg aactatttta tatggtttat taataaatag
    11821 aatgtaaaga ttttgtaaat taccctgtac ttttaataat atcataacaa acgaaaaata
    11881 aatagactat taaaaataat cattttcata tacaataata ttaatttaaa tacaataaat
    11941 ttataaaaat tgtatttaaa gattaacata ttaaagtatt agaattggaa aggcttgaag
    12001 ggagaggttt ttatgcaaat aatagtatct gggagacaaa tgaaattaac tgacggaata
    12061 aaaggatacg tagatggaaa attaagtagg ttagaaaaat accttgatcc tgaatcagaa
    12121 gtgaaagtaa cagtaagtgc gaaaaaggat agacaaaaag tggaggttac aataattcct
    12181 attaacggtc aaataataag agcagaagac gtagaagatg acttatatgc agcaattgat
    12241 attgtttgtg acaaattaag tagacaagtt gtcaaatata aaactaaagt taaagacaaa
    12301 gttcaaaata ataagagcat aagatttgaa aatcttgact ttatagataa tagcagtgaa
    12361 tttgatgatt atgattatga tgatgaagaa gatgagaata tagtaataga aagaagaaag
    12421 aaatttaatg taaaacctat gagttctgag gaggcgatac ttcaaatgga actagtagga
    12481 cacaatttct atatgtttag aaaccaagat aattttgaaa taaacatagt gtataagcgt
    12541 aaagctggtg gatatggttt aatagaacaa gattaataaa aattgtatac gtttagattt
    12601 tatataagaa aaaataaaaa ggtaatcatg ttgatttttt atcaatatga ttaccttttt
    12661 cttgtttaaa taatatatta gatattaggt atttattata taaaaatttt aaaactaatc
    12721 tgttacaaca tcaataaagt gcataaatac ctatttcaag cataaaatta attaaggggg
    12781 taatgcttat gttagaaata tccactgatt tacaagtaaa tcaaccagtt attactgtta
    12841 caagtaatac gtttattagt atagaaaatt atctatcaat attagaatat gaagttgatt
    12901 taataagaat aaagactaaa gttaaaacaa ttaaaatatc tggggataaa ctttcactta
    12961 agtatattac tgatagtgag ataggtataa aagggattat ctacaatgtt gagtatgttg
    13021 attaggagga gatacacttg ataagtttta taaggggata ctatgttata gttgtggaag
    13081 gagtaggact cgaacaattt ttaaatcatc ttataagaaa tgggataagt gtttacaatg
    13141 taactagaat aaaaaatact aaaatggagt ttcatataga tagacaagat ataaaagaat
    13201 ttaagaatgt ttatagagga agtaagtttg atataaaggt aaagcaaaaa actggggtcc
    13261 cttttattat caaaagagtt tataagcaca aaggtatgtg gatttgtgct cttgtttctt
    13321 tatttttatt gatgtctaca tcacagtttg tgacagatgt atatatacaa tctcctgaag
    13381 gtataaaaaa agaagctctt agaaatgagc tttataaggt tggagtaaga ccaggagtct
    13441 ataagaagag tattgataga aaagaagtta gagaccatat gatgtcaaaa tttaatgatg
    13501 tggcatattt atctatcaat gtaaaaggaa caaatatatt tgttactgtt acaaaaaagg
    13561 cagaatccct aaaatctaca gaccaatcaa attattgtaa tgtaattgct ttaaaaaatg
    13621 gtataataga aaaggtaatc cctagaagtg gtaaatcagt tattaaatct ggagacatag
    13681 tccaaaaggg agatgtctta ttaaatggag ctaataccaa gtcaatacca gaagtatggg
    13741 caagtacttt ttatgagtcc acaaaaaagg caagttatgt agatactgta aataaaaaaa
    13801 ctggagaaaa gaaaaatata tatacactaa gtttttatga taaagagttt acaatgagaa
    13861 aaaatataaa atataaagat tatgttgttg aaaataaaga aaaaaaacta tcaataggaa
    13921 actatacttt tccaataaag ataaaaacga gtacttttta tgaaacaaaa aaggttgaag
    13981 taaaaaggaa taaagaagag ttaaaaaaag aattatctga aaaagcttta aaagagttag
    14041 aatacataat cccagcttcg gctagaataa tagatgtaaa acataacttt aaggttaata
    14101 aaaatatgtt agaatattta ataacagttc aaactagtga aaatattgct aaaatttatc
    14161 ctcttagtaa gtcagaagca gaaaggttta taaaggaaga aagtaaacca gatgaaggtg
    14221 aagaggaagt tccttccaat ccagaaaaaa gaccattaaa tgatataaga aatgagtttg
    14281 atgaggataa taaggataat aaagatcaaa acaattctga tgaaaataat agtaatcaaa
    14341 atagtaataa tagtcaaaat aataatagca ataacaacta ataaaattaa acttaaaatc
    14401 ttaggaggac taattcaatt gataatacaa aagaaattta caatagcaga tggtaacttt
    14461 gaaagagagt tatttggcaa ctttgatgaa aatgtaaaat taatagagaa gactttaaat
    14521 atagatgtaa tacttagaga aggcaatata attttaattg gagaagaaaa aaatgtagat
    14581 tctgcattaa aacttatgaa tgaattacac caaactgtat caaatggtaa gcatttagat
    14641 aagcagagta tttcttattc attatctctt ttgcttgaag gaagtgaaca aaaaataaaa
    14701 gaacttgagg ggacaatcgt cataactcaa aggggaaaag ctgttcaacc taaaacttta
    14761 ggtcaaaaag aatatataaa acttatagaa aataatgata taacttttgg tgttggacct
    14821 gctggaacag gaaagactta tcttgcggtt gctatggcag taaaagcttt taaaagagat
    14881 gaagttagta gaattatact tacaagacca gctgtggaag caggagagag tctaggtttt
    14941 ttaccaggag atttaaagga caaagttgac ccatatttaa gaccactata tgatgcctta
    15001 tttgaaatgt tgggtgctga taaatttaat aagtatttag aaagaggtac tattgaggtt
    15061 gcaccacttg catttatgag aggtagaaca cttgataatt catttataat attggatgaa
    15121 gctcaaaata ctacttctga acaaatgaaa atgtttttaa ctagacttgg atttggctct
    15181 aaagctgtag ttacaggaga tgtaactcaa acagatttac cacaaaataa gaagagtggt
    15241 ctaatacaag ctacagagat tcttaaaggt gttccaggta ttgggtctat aatgctaaca
    15301 gatagagatg ttgtaagaca tgagttggta caaagaataa taagagctta tgagaagcac
    15361 gacaaaagag aagaatttaa aaaagaagaa cgtaaaaaag cgaaaatgca ggaaaagact
    15421 ttcaaaagaa agtaaaagtg tagtaatata gatatatagc attattaatg aataaaaagt
    15481 aggtgtagtg aatggatttg atactagatg atagacaaga taaattagaa gtaagtgagg
    15541 agttaattga aaagataaag gatataatca tagaatgttt agattatgaa ggatatgatg
    15601 ataattatga agtgagtctt tcttttgtag ataataaaga gattcatgaa ttaaatagag
    15661 aatatagagg agttgataga gtgactgatg ttttatcatt tcctctttta agtgatgatt
    15721 ttgaggatgt tgagttagaa gaagaatccc taggtgacat agtagtatct cttgaaagag
    15781 ccttagaaca gagtatagag tataaccata gctttgaaag agaagtatgt tttttaatat
    15841 gtcatagtat gtttcatctt ttaggttatg accacgatac agatgaaaat acaaaagaaa
    15901 tgagagaaaa agaagaacac atattaaata agttaaacat aacaagggag tagtttttat
    15961 gaaaccagaa aaaactcgtc aaggtataat taaagcattt aatgcagcta tagaagggat
    16021 attgtataca tttaaatttg aaagaaatat gaaaatacat tatttaggtt cagttgctgt
    16081 tcttataata agtttgtttt ttaacttttc aaaattagaa atgataatgt tacttatgtc
    16141 catatgctta gttgtagttg ctgaaatgtt taatacagct atagaaaaag ctgttgattt
    16201 agtaactgat gaatatcatg ttcttgctaa gatagcaaaa gatgttgcag ctggaggcgt
    16261 tctagtggca gcactaaatt cagtagtagt tggatatatt ttgttttatg ataaattgac
    16321 agatatatct ggaatattaa tttataaaat aagagaatca gaattacata taaccttgat
    16381 atgtatatta ttggttctta tagctgttgt agttgtaaaa gcattgactt ctacaggtac
    16441 tcctctaaaa ggaggaatgc caagtggtca tgctgcctta gcatttgcta tagcaactgc
    16501 aattacactt atgactgaaa gagttgttgc ctctactctt gcatatataa tggctgtatt
    16561 agttgcccaa agtagaattg aaggaaaaat acataccttt tgggagacta ttgctggagc
    16621 tttgttaggt gtgttgatag ctatattagt atttcagctt ggaatgttct ataattaaaa
    16681 taaatgtaca taaaatattt aaaataaaac aaatctgtgc taattaaatt tttatgatat
    16741 aatgtagagt attaaataaa aataactttt aattattgta tacaggagga gttgttgtgg
    16801 ataacataga attgttaagg ttggcagaag atgccagaca acattcttat gctccttact
    16861 caggttttag agtaggtgca gctcttttaa ctaagagtgg taaagtttat actggatgta
    16921 atatagaatg tgcatcatta ggtggaacaa attgtgcaga aagaacagct atttttaaag
    16981 caatatcaga gggtgataag gatatctata aaatagctat agcaagtgat aactcagaaa
    17041 ataatgagca aacatatcca tgtggaatat gcaggcaggt aattatagag tttggttctg
    17101 atataaaaat aataacggga tatactaaag gagaggtgtt tgagcatagt ataaaggatt
    17161 tgttgccaaa ctatttctct ggaaatgatt taaaataaat ttaaaagtat aaataaattt
    17221 aagtaattaa aacaagaaga taaaaagtta ggagacagtt aatgttcaaa tcaggttttg
    17281 tcagtatagt gggtagacca aatgtaggaa aatctacttt aatgaataat gtagttggag
    17341 aaaaaatagc tataatgagt gataagcctc aaactacaag aaatacaata caagcagtat
    17401 atacagatga agaaatgcag atagtattct tagatacacc aggtattcac aaaccaaaaa
    17461 ataaattagg tgaatttatg gtaaaggcag caacagaagc ttttaaaaat gtagatttaa
    17521 tattatttgt agttgatgat tctaaaaaaa taggtcctgg agatagaaag ataatagagg
    17581 atttaagaag tgttaagaca ccaataattt tagttgtaaa caaaatagac caactagacc
    17641 aaaaagatga gctatttgat ataatcaaaa tgtatgatag agaaggcatt tttaaagaaa
    17701 tagtaccaat atcagcatta aaaggaaaga atacggatac cttaataaaa gttatacaaa
    17761 attatttaga agaagggcca aagtattttc cagattatat gattacagac cagccagaaa
    17821 gagtattaat agcagaactt ataagagaaa aagttcttca ctatcttaat gatgaaatac
    17881 ctcatggtgt agctgttgaa attgaaaaaa tgaaagcgag aaatgataag gaaatagtag
    17941 atgtatctgc tgtaatatac tgtgaaagag attcacataa gggaataata ataggaaaaa
    18001 atggtaggaa actaaaagga ataggtaagt cagcaagaca agatatagaa ttgcttttag
    18061 gttcacaaat aaatcttcaa ttatgggtta aagtcaaaga aaattggaga aatttacaaa
    18121 attatataaa taattttgga tataatgata aataaaggtg ggttatatgg ataggaaact
    18181 ggatgcttcc caagatttat tatatgaagt aaaatcatta attgataata ataaagtatt
    18241 ggagttaaga gaattaatag aggaatatca tataattgat atattcgata taatggaaaa
    18301 tttagaggaa gacatgaaaa ttcaattatt tgaagtactt cctttagata tggcatcttc
    18361 tatattagaa gaaggtagtg tagagttttt tattagtatt ttatctaaac tggatgtaga
    18421 acactcgaaa aatatacttg aacttatgtc tcttggagat atggcagaca agttgagtga
    18481 acttgaagaa gaagaaagag aacacataat aaatcttttg aatcaagaga atgccgatta
    18541 tgtaaaagag ttactattct atgatgaaga ttctgctggt ggaacaatga ccactggata
    18601 tatttctata aataaagata tgacagctct tgaggctata gaccatatga gagaagaagc
    18661 tgaagaagct gaaactattt attatatata tgtggttgat gatgaagaaa aactagttgg
    18721 agtattgtca ttaagagaat taataatagc tagagacgca aatatagtag aagatttgat
    18781 gagtgaaaat ataatctctg tttatgtaga tgaagataga gaagaagcag taagattagt
    18841 atctaaatac aatttaatag caattccagt agtagacaga caagaaaaat taaaaggaat
    18901 aataacagta gatgatataa ttgatgtaat ggaagaagaa gcaactgaag atatgtataa
    18961 atttgcagga agttcagaac atgaaagaga agttgctgaa aaagaaaacc ctacattgag
    19021 agagcaaata atatcagcac ttagaggacg attaccttgg ttaattatta ccttagttgg
    19081 agggttgttg gcttcgttaa tactttctaa cttagactat ataatgaatc ctgtatatgc
    19141 tccattagta ttttttatac ctgtagtaat tggtatgggt ggaaatattg gaactcaatc
    19201 atcatctgtt acagtaataa cattatctaa taaggattta aattttagca atgtggttag
    19261 agaaggtatt gtaggtatta ttacaggtct tttgtgtagt ataataacag gtattgtaat
    19321 ttactttgtt atgagaaagt tagacattgt attaattgtt tcaatatcat tatttataaa
    19381 tatggtttta ggtgcaacaa taggtgcgtt tatgcctgtt ttattaaaaa aaatggatgc
    19441 agacccatct acagtttcat caccaattat atctacagct cttgatataa ctggtatagc
    19501 agtttacttt ataataacaa cggcattatt gtcaaaaatt gtttaataac tttatgtatg
    19561 atttatcctc ttcttactaa aaatataagt ataaatgtaa gaagggggtt ttttaaaaat
    19621 gaacaatatc agttgggaag tttttaaaaa aacaggtagt atagatgcat atttatattt
    19681 ctgtgattgc aaaaacatac atgaagaaat tgaagaagta agggagaaaa aagaagacga
    19741 tgataatcct gaacacccag gggatagtac ttaaagctat aaggtataag gaaagtgata
    19801 taatacttac actatttaca agaaagcttg gaaaagtatc tgcaatagca aaaggagcta
    19861 aaaaaaataa gagctctttg ctttcatcat cacagttatt ttcttatagc aattttacct
    19921 taaaaaaaca aggtaatatg tacaaggtta ctcaaagtga aataataaaa agtttttata
    19981 atatatccta tgatatagag gcattttctt atgctacata tattacaaaa ttagttgaaa
    20041 attctatact tgaaaatcag actaataata ggctatttat attacttgcg cagactctgt
    20101 atttgtatac acaggataat acagacaata ggtttataac agcagcattc gagttaaaat
    20161 ttttagacta tataggattt aaacctatag taaataaatg cattaattgt gaatgtaaaa
    20221 ttttacaaaa ttctgtgttt aatatctacg aaggtggtat actatgtagt aagtgtagta
    20281 aactttttga taataatata aaattagact taacaactat aagtcttatg gaatatgtat
    20341 taagaaatga tattttaaca tgtagtaaag ctaaagtatc aaaatatata acacatgaac
    20401 ttgagaatat tctagacaag tatttgaaag tttatgttga taatattaat tttaaatcat
    20461 tacacgtatt gcaaagtgta aaaaataata agggagtgga taatgatgag tagtaatata
    20521 actattgaaa tggttgattc agtctttgaa agagttccag gagcaactta tgcagaagca
    20581 aaagaagcat tagtaatgtg caatggagat gtaattgagg cagtaatata tttagaaaat
    20641 aaaaaaaata cttgtaattg taaagcaaaa tctgctaaag aaactatgga agaagtattt
    20701 ggcaaagatg gagaaaatat aaagtttcaa ttaaaagagc ttcttagaaa aagtagtgta
    20761 gtgagaataa tagttgaaaa agatggaaaa actatgatga atattccttt aacagttgga
    20821 gttgttggat tagcttttgt accattagct actttagttg gattatcagc tgctgttata
    20881 agtaaatatc gtataaaagt tcaaaatgaa gaagatggag aaattgtaga tttaggtgaa
    20941 ttgaatgaag aaaaacttca cgttttgaag aatatgataa ctaatgcagc aaaagatgtg
    21001 aaggatgtag tagttgacaa aaaagaagat gacaaagatg taactgctga ccttatgaaa
    21061 gaagaatctg aaaagaataa agaagacgaa gacgaaaata aatagatata aagtgacttt
    21121 taaaaaatga agagaagata attataagaa ttatcactaa cattgacaaa ttttatagaa
    21181 tttgatattc taaaagttgt agaagaacta ttaaaatagc ctttcgtgta gacgagaggc
    21241 tttattgtta taggagggat ttttatgaat tttcaagaga tgatactggc gttgcaaaaa
    21301 tactggtcaa agcaaggttg tatcatgatg caaccttatg atattgaaaa aggtgcggga
    21361 actatgaatc ctaatacttt tttaagatca ttaggacctg agccttggca agtatgctat
    21421 gtagagcctt caagaagacc agcagatgga agatatggtg aaaacccaaa cagactatat
    21481 caacatcatc aattccaagt tatactaaaa ccttctccag ataatataca agaattatat
    21541 ttggaaagtt tgaaagagat tggaattgac ccaagtgagc atgacataag atttgttgaa
    21601 gataactggg aagctgctac agttggtgct tggggacttg gatgggaagt ttggctagat
    21661 ggtatggaaa taactcaatt tacgtatttt caacaagtag gtaatattga atgtgagctt
    21721 gagacaggag aaattacata tggattagaa agattagcta tgtacattca agaagttgat
    21781 agtgtttatg atttaaaatg gaatgataaa ataacttatg gtgaagtatt taataaagct
    21841 gagtatgaaa actcaatgta cgcatttgaa ttatgtgatg cagatatgtt gtttaatctg
    21901 tttgatatat atgaaaaaga agcattaaga cttatggaaa atggccttgt aataccgtca
    21961 tatgactatg tgttaaaatg ctctcatgca tttaatacat tggatgcaag aggtgcaata
    22021 ggagttagtc aaagagcttc atttataggt agagttagaa atatggcaaa gactgttgca
    22081 gaaacttttg taaaacaaag agaagaaatg ggattccctc ttttaaagga cggtgacaaa
    22141 taatgaaaaa ttatctttta tttgaaatcg gtgttgaaga aatgccatca agatttgtag
    22201 gcagtacact agaacaatta aaaaacaatc ttagtaaatt atttgatgaa aatagaatag
    22261 cttttgataa aataaatgct tatgggacac caagaagact tactttagta gtcgaaggat
    22321 tgagtgatag acaaagtgat ttagaagagg aaataaaggg tcctgctaaa aaaattgctg
    22381 ttgatgcaga aaataattta acaaaaccag cattaggatt tataaaaagc aaaggattaa
    22441 gcgaatctga catatatttt aaacaagttg gaaaagaaga atatatattt gcgactataa
    22501 agcaagatgg aaaagaaact tcagaagtac taaaagacat attaccagag actataaaat
    22561 ctgtagtatt cccaaaagct atgcgttggg gaggaaaaaa tcttaaattt gcaagaccta
    22621 taagatggat agttgcattg ttaaatgata aagtattaga gtttgattta gaaggaataa
    22681 tatcttctaa tgtaacaaaa ggtcatagat ttttaggtga aagtacaata gaagttgatt
    22741 ctatagaaga ttattttgaa aaattagaaa aaaattatat agttttagac caaaacaaga
    22801 gaaaagaaat gataagaaaa caatgtatag aagtagcaaa ttctttaggt ggagaagttg
    22861 aatttgatga agatttactt gaagaggtaa cttatttagt tgaatatcca actgcttttt
    22921 atggagaatt tgatgtggat tatataaaac taccaaaaga agttgttata actcctatga
    22981 aacaacatca aagatatttt ccagtactta aagaaggaaa actgttacct aactttatag
    23041 ctgttagaaa tggtgattct tacagaatag acaatgttaa agctggaaat gaaaaagttt
    23101 tagaagcaag acttgcagat gctttatttt tctatagaga agatactaag agaaacttag
    23161 aaagctacat agaaaaatta aaaactgttg tattccaagt aaaattagga actatttacg
    23221 acaaaacttt aagacttgaa aaattaagtg ctgatatact tgatagttta ggtttagtag
    23281 atgaaaaggc agatactata agggctgcta aattatctaa ggcagattta gttactggaa
    23341 tggtaggaga atttgatgaa ttacaaggtt ttatgggaaa agaatacgca aaagttggag
    23401 gagaaaatga tgttgtaagt gaagctattt ttgaacatta tcttccaaga tttgcaggag
    23461 atatacttcc taagactaat gcaggggttg ccttatcaat agctgataag ttggattcaa
    23521 tagcaggttt ctttgctata ggaatacaac caacaggttc tcaagaccca tatgctttaa
    23581 gaagacaagc tttaggtata cttagtatac ttatggacag aaaagttgct gtggatataa
    23641 aaatacttat tgaacatgct ttagacaatt attctgaatt agattttaat aaagaagaag
    23701 ttgttgagca aataatgaat ttcttcaatg agagagtaaa aaatctattt aaagatttag
    23761 gaataagata tgatgttgtt gatgctgttt taagttctga tataaatgat gtttctgata
    23821 tgcatatgag agctttagag cttaataatt ggctagaaaa agatgaatta gtggagatgc
    23881 ttactgcatt taatagagtt tctactctag ctcaaaaagc agaaagtgat aaaattgatg
    23941 aatctttatt aaaagaagat gctgaaatta aactttacaa tgaatttaaa caagtaaagg
    24001 taaaagttaa tgaattatta aaggataaga aatatggtat tgctttagat gaatttgcat
    24061 ctttaagacc agtagttgat aatttatttg atacagttat ggtcatggat aatgatgaag
    24121 ctattaagaa caatagactt gctttactta aacaagtata tgatactatg ctaggaattt
    24181 gtgatttatc taaaattgtt tataaataaa atttaaacta tttataagaa ataaggctgt
    24241 ctcaaaatag agatagtctt atttttcatt gttgagatga tgaaaatata tttgatttca
    24301 tagaaaaaga gtgtctcata aactaaaaag ttcattttga gacacccatg ttttttgtaa
    24361 gtaaatataa taaattatat tttatactta tcagttattt tgtttaaaat attatcattt
    24421 gaatttaccc attcataaaa attgcctgaa gatgcgtccg atattttata aagcatttca
    24481 ggaatatcac tagctttaaa atctccaagt gattttccca atctagtctt acctatttca
    24541 aaactgcctc caacaaataa ttcaaatgca tctgtagata taccatcaac attcttcttt
    24601 ttaccacata aacctatgct accaacttga tgaacaccac atgaatttaa acatcctgat
    24661 atatataact taggtgcttt atttaaaatt tcttcattat tttgtaatct aaaataatct
    24721 atgatttcat gtaacatttt ctgactattt tgtatgccca tttgacaaat tggaacacct
    24781 atgcaagata tactttgttc taattgagaa ttacaactaa tagttttact tatttctaat
    24841 attctttttg cttcatttcc atttaaattt aaaatatata atccctctgt cattcctagt
    24901 ctaatcatag gattttcaac attatctaat tctttaagta aagtacttaa atcctttgtt
    24961 aacaaaatac ctccaactgg atgtatatat acagtgtata aaccatcctg tttttgaggt
    25021 attagtctag aatcttgtat atctatttta actccaggtt tagagtaatc tatttcttca
    25081 atattgagtt caagactacc attttctttt tctaatttat agtattcttt aaatttttct
    25141 aagaatagtt cttcaccaag tgattcgacc atatatctaa ccctagctct gtttttattt
    25201 tcataatttc cataatccat aaagaattta atcatacctt caacacaata taaggcgtct
    25261 ttcggcttta taagttcatc aagttctaaa gcaactttgg gttgtttacc tagacctcca
    25321 cctaagaaaa ctctgaaata tggttcatca tttttcaaag tagcaacaaa tcctaagtct
    25381 tgtatactac aatgagcagt atctgtgtaa caattagaat aagaaacttt taattttcta
    25441 ggtaaatggt atgtagtagc atttttgata aaatatttat cagtagcaac tgcatatgga
    25501 gtaacatcaa agacttcact agggtcaact ccagataatg gagataatcc gacatttcta
    25561 ggataattac caccaccacc tctagtgaat atatcatttt ttattccttc ttccatgata
    25621 tcaacaattg aatttataga taaatcgtgt aattgtatag cttgtctagt tgttaagtgt
    25681 atcttatcta agttgtgttt aatagctatt tcatagattt tattaagttg atactgagat
    25741 aaaactccag cagatactct aagtcttatc ataaaagatt ttttgtctct ttgagcataa
    25801 acaccatatc caccagaaaa tcccttatat tgcattttat ttatttctcc atttaaaaat
    25861 tttgaagaat attctttaaa attaggtagt tcatttaaca atatcttttt aaaattatcc
    25921 attatttcac ctcattgtta gtcttgtata aaaatatatc tattgtataa tagatatata
    25981 tattattatc tagtcaggat aaaaatataa cagaataatt ctattaaaag taagaataat
    26041 aaggacatag tgagtatttt gtttttattt tacaaattaa gtataatatt tttgttttat
    26101 aaaatataat taagataaat agataatttt gtaattttaa tatataatat ttagtagagt
    26161 gtgggagtag cacttaatat ttgcaggagg agtttttgta tatgggagaa ataataaatg
    26221 cattagatag agccctagac ataattttgc tgttatatca tgaaaagaga gaaatgggaa
    26281 taactgaaat aagtaaggct atgggagtat ataaaagtac tgtacataga actttggtta
    26341 ctcttgaaaa caaaggtttt gttatacaaa atgcagagaa tggtaagtat tggctaggaa
    26401 taaatttata tgcaataggt atggttgtgg gagaaaaaat gtcattgact gaaatagtaa
    26461 aaccatatac gaaaaaatta aatcaagaat ttaacgaagt agttaatgta tctatattag
    26521 aagaaagagc tcaagattct ccaagaagta tcataataca taaagagtat ggttcaaatc
    26581 aattactttc agtaaatcca tccgttggtt caagtagtga atgttattgt tcagcagttg
    26641 gtaaatgtct tatggcattt aatgattcta tagattttga aaaatataga aaaacaccaa
    26701 tacacaagta tacagaacat acaattgata attgggatga tatgatgctt tttttagcaa
    26761 aaataaaaga acaaggatat gctatagatg atgaagaact agaacatgga ctaacttgta
    26821 ttggagcacc tatcttagat aaaaataata aagctattgc agccataagt ttgtcaggtc
    26881 caactataag aatgagagaa ggggactttg agtataagat taaaagagtt atagaaactg
    26941 caaaaagtat atctgaactt tttagatgat ttttaatctc gataataaaa ataggctata
    27001 tttaaattgt tatttgtaaa tatcagttta aatgtagcct atttttcttt aatctaaaat
    27061 taaatattct taaaatttaa aatgttattg aaatatataa gcgaatgtgt tatcttttaa
    27121 ttaagcaata aaaatagaac aaggttctat actatagaac aaaattgatg catatatagt
    27181 atttatacaa aatataaata ttatatattt aaaattaagt aaaaatatta cataaagggg
    27241 agatttgaga aatggaaaaa gtcaagttga gtgttgaaag atgtaaggga tgctatcttt
    27301 gtattgaagc ttgcaaaaaa gatgcaataa gtctttctga ctacacaaat aaaaaaggct
    27361 ttgtaactat taaagtcgat gaagaaaaat gtattcaatg tggcgcttgt tatacaatgt
    27421 gccctgattt agtatttgag atattataaa ttatttttat tagaggggag aaaaggtatg
    27481 caaagtttat taattaaagg aaatgaagct ttagcagaag gtgctatacg tggtggttgc
    27541 agattttatg caggttatcc aataacgcca caatcagaaa tattagaaat gatggcatgg
    27601 agacaaaaag aagttggagg agtatttatc caaggtgaat cagaaatagc cagtgtaaat
    27661 atggttatgg gagcaagtgc acttggagca agagctatga cttcttcatc tggtccagga
    27721 ttttcattaa aacaagaagg tattgcatat tgggtatcag ctggtttgcc agcagttgta
    27781 gcatgtgtgc aaagatatgg tataggtgat ggatttatag gagctggtca agataattat
    27841 tggcaagcag taaaaggcgg tggaaatgga gattatcatt taatcgtgtt agcacctaat
    27901 agtgttcaag aaaatatgga tatggcatat gaatcttttg aattggctga aaaatatatg
    27961 catccagtac ttatactttc tgatgctaca ataggtcaaa tggtagaacc ttgtatagca
    28021 ccaccaatga aagaatacga tatggataaa gctccatggg ctgcaaaagg aacacctgta
    28081 ggagaaaaaa ataaagtaat aactgatata acatattttg aagaccaaga tacatggcaa
    28141 aaagggtata tagaaaaaat gagaaaagtt caagaagatg aacaaagatg ggaagagata
    28201 gaaatagaag atgctgaagt ggtatttgtg gcatacggta tttcatcaag aatagcagaa
    28261 ggtgcagtta gagatgctag agcaaaagga atgaaattag gtctaataag accaaaaaca
    28321 ttgtggcctt acccaagaaa agcatttaag aaattaagtg attcattgaa gggacttgta
    28381 acagttgaaa tgaacatgat ggcacagatg aaggaagatg taattatagc atgtgataat
    28441 agatttccag tatattcagt agcaacagct caatatatgg cagaagtaaa tcaattaata
    28501 gattttgctc aacagattat agatggaaaa gctaagcaag aggaggtatt ttaattatgg
    28561 ctaaaaaagc accagcaata atgatgtgtg aaaatatgtt ctgtggaggc tgtggacact
    28621 ctatagtcga tagaattatt ggagaagtat taacagaaat ggatatatgt caagatacaa
    28681 ttatatgttc agatataggt tgtaatcata tgtttcaatt tagtatgaat gtagatgttg
    28741 tcgttccacc tcatggtaaa atgggagcag caatggcagc tatgaaaaga gttagaccag
    28801 ataaatatat atttacaata gctggagatg gtggagctta tgcaataggg cttggagaaa
    28861 ctatgtcagc tgctactaga aatgataatg taatatttct tgttatgaac aataatatgt
    28921 ttgcaatgac aggagggcaa atggctccaa ctactataga aggtcaatat acagcttcta
    28981 ctccagcggg tagaaattac gcagatcatg gttcaacatt tgatgttgta aaagttatgg
    29041 gaaatttaga tatagcatat ctagcaagag gaacaataac taaccctaaa gaaataaata
    29101 caacaaaaag atacatcaaa aaagctctag aaaaacaaaa agaaaagaaa ggtttttgct
    29161 taatagaagt gcttgtacca tgtccaacaa actggggatt aaagccagta gatgctatga
    29221 agagaattga aaatgaactt gttccaaagt atccacttgg agaatttatt gattagggag
    29281 gaaataatat gttgaatgaa attatatgtg ctggttttgg aggacaggga gttcttacag
    29341 caggaaaaat tataatgtat gttgcatata aggcagaaaa gaaaattaca tggttcccat
    29401 cttatggaaa tgagatgagg ggaggaagtg caaattgcaa tgttgttgta agtgatgaaa
    29461 aaattgcaaa tccttgtgta aaaaatccag atatactatt gggaatgaac agcgtatcag
    29521 tagataagta tgaaagttta atgaaaaaaa atggttatat gtttgtcaat gaatcactag
    29581 taagtaagga tagaaagtac agagaagaca ttaatgttat aaaagtacct gttactgaaa
    29641 tagcacaaaa tttaggcaat gaaaaagctg ctaacatttg catgttaggt gctatggtaa
    29701 agagtttgga tttatttaca aaagaagaat ttgaaagtgg tatgtgtgaa tactttgaaa
    29761 atcaaggcaa aggtaaattc aattctaaaa atgtagaagc atttgaagct ggatacgact
    29821 acgaatgaat attttaaata tttaaaataa ttattgaaat acttattttt atatgttagt
    29881 atttaattaa taaaatatag aacgtcgttc tgaacaatag gacgaagtct ttaggtcgga
    29941 atacaagaac gacgttctgt atagtagaac tagggggtgt taaattgaaa gttttagtaa
    30001 taaatcctgg tggtacatcc acaaaaattt ctgttttcca agatgaaaat gaaattttga
    30061 aaaaaaatat aacccataca agagaagatt taaaaaattt ttctaaagta tttgatgaat
    30121 atgattatag aaaacaactg atagtagata ttttaagtag tgagaatcat agtataaatt
    30181 cttttgacgc agtagttggt cgtggtggac ttatgaaggc tataaaaggt ggtacctata
    30241 ctgtgagtga agaaatgatt gaggatatga gaaatgaaat aaatggggaa catgcatcaa
    30301 atttaggtgc attattagct aaaactatag ctgatgaaat aggagtacaa tcatttgtag
    30361 tagacccagt ttctgtagat gaatttgatg atgtatcgag gattacagga atatcagaca
    30421 tagaaaaagc aagttggtta catgcattaa accataaagc tgtttcaaga aaaattgcac
    30481 aaaaactagg gggaaaatac gaggattata actttattgt agctcattta ggttcaggaa
    30541 tatcgattgt agcacataaa caaggcaaaa tgattgatgg aagtggtggt aggtctgatg
    30601 gtccattttc tccagaaagg agtggtggat tacttgcata tcctcttata gaattgtgtt
    30661 actccggaaa atatactcat gatgaaatgg tcgcaaaagt aagttcgatt gggggaatgt
    30721 acgattattt aggaacaaag gatatgatag aaattgaaaa cagaatcaag gagggtgatg
    30781 aaaaggcaaa acttataatg gatgcattta tttatcaagt agcaaaagaa atttctatgt
    30841 atggagcttc actttcaggg aatgtagata gagttatctt gacaggaggg atatctcact
    30901 cagaaatggt agtcaaagga gtagcagaaa aagtttcata tctagctcca atagagatag
    30961 taccaggaga gatggaaatg gaggcacttg ctctaggggc acttagagta cttaatggag
    31021 aagaaatagc aaaaacttat taatatttag gagaaagagt atgttaaaag attttaataa
    31081 tttattagaa gaggctaaaa agcttgaacc tgttattgta agtgttgctg ttgcagaaga
    31141 caaagaagta ttaaaggctg tgaaatcagc aacagattta aacattataa agccaattct
    31201 tgtaggagac aaggataaaa tagaaagtat atcaagagaa attggattaa aaggtcatga
    31261 aataataggt tgtcaaaatc ccgatgaatc agttaaaaaa gctgtagaga tagttaaaaa
    31321 taaggaagct aaagttctta tgaagggatt agtaaataca agtgtctata tgagagctat
    31381 attaaataaa gaaacgggtc ttagaacagg aagattatta agtttgcttg cagtatatga
    31441 attacctcaa tatcataaac ttctttattg tactgatagt ggaataaatg tatcgccaaa
    31501 cttaaatcaa aaaaaagata taatgacaaa tgcacttctt actatgaaaa gtataggcat
    31561 ggaaaatcct aaaactgcga tacttactgc aaatgaaatg gtagacccta agatacaatc
    31621 tacagtggat gccaacctac ttgtagagat ggaaaaatcg ggagaaatac caaagtgtat
    31681 agtggaaggt ccaatagcat ttgatgtagc atttgatgca catgcagcag aacataaagg
    31741 tattaaaagt aatattgctg gtgatgttga tttattagta tttccaaata tagaagcagg
    31801 gaatatatta ggaaagtcat ggcttagatt taataaagct aagtgggcag gtatagtttt
    31861 aggagcaaca aatccgataa ttttaggttc acgttctgac acagctgaaa taaaaataaa
    31921 ttcaatagca ttagcgtgcc tagcatcaca ataagaaatt attattaagg aggaaaaagt
    31981 attatgaaat atgcaaaaag aatggatatg gtaaaagctt cagctataag agattctcaa
    32041 aagaaaatag ctcagaaggt agcaagtggt ggaactgtta tatcttttgc tgcagggctt
    32101 cctgacccaa atttatttcc aatagaagag ataagtgaaa taactcaaaa agtacttaat
    32161 gaaaggggaa aatttgccct agcatatgga cctactaaag gtgaagatga gttgttagat
    32221 ctattagtaa aaagaatgaa agaagaagaa aatattgata caaaagcaga aaatattata
    32281 ataactactg ggtcacaaca agggatagca ttaagtgcta tgattttatt agaaaaggga
    32341 gatgtagtag ttacagaaaa tccaagttac ttaggtgcaa taaatgcatt tagaccttat
    32401 gaatgtgatt ttttaggagt agatacagat gaagaaggaa tggtaacaga tgatttagac
    32461 aaattattat ctgaaaattc taacataaag gtgatatatg taataccaac atttcaaaac
    32521 cctacaggaa aaacatggtc tttacaaaga agaaaagatt ttatggaagt cgtgaataaa
    32581 tatgatgtca tagtaataga ggataatcct tatggagaaa taagatttac accagaacct
    32641 ctacctacac ttaaatctct tgatactaaa gataaagtat tgtaccttgg gtcattttca
    32701 aaggtattat gtccaggatt tagagttgct tggatgtgtg gtaatgtaga catcatagat
    32761 aaggcagaac ttttaaaaca aggtatagat ttacagtcaa atcaattttc gcaagtacaa
    32821 gtaatagaat ttttaaagaa atacaactta aatgaacata tagaaaaaat aagagcagag
    32881 tataagaaaa gatgtctgct aatgttagat gctatgaaga aacatttccc agaggaagct
    32941 aaatatacag aaccagatgg aggaatgttt atatgggttg aacttcctga aagtataaat
    33001 gtagatgatt tattagataa agccattgat gctggagttg cttatgtttc tggagagtcc
    33061 ttctttgcaa ataatggtcc aaaaaatact atgagactta actatacaac aatgtcagaa
    33121 gaacaaatcg ttaaaggtgt tgagatactt gcagaagtta taaaaaaaga actcaattat
    33181 gcttgtgaat aaatataaaa agtgagggtt taaatatggg agaaaaagaa aacggtataa
    33241 agtttggaaa gttaatgttt caacttgtta ttatagcaat atcttgggaa ttagtttata
    33301 ttataccatt tatccaatat acattatatg atccaattct aaaagcatta gaatgtacaa
    33361 acactcaact aggttttcta cttacaattt atgggttagg taatatattt ggagctccta
    33421 taggaggctg gttagcagat agatttgatt atagaaagat aattttaggt tctgtatttc
    33481 ttaatggagt agtatcattt ttatttgcat ttaatatgaa ctatccattt gcagttgcta
    33541 cttgggtagg ttgtgcaatc acatcattag ttatgaacta tccttctatg gtaaaaatac
    33601 ttagagttat aggaaaagat aatcaaggta aggtttatgg atttaatgaa gctatggttg
    33661 gagtttctgg agtagtaatg ggagcaatat tcttatatat ttatacatgc tttgcaactc
    33721 ctacattggg tatgagatgg gtaatgatat ctttaggaat attatcaata gcaatgtgtc
    33781 cagtattatg gtttgtaatt aaagatgtag atattaaaga agaaagagaa gaagcaggtg
    33841 aaaaaatgtc tgctggtgat tttataacag tactaaaatc accaaatact tggctagtag
    33901 gaataagtat attctgtgta tattcattca cagttacaat gtcttatttt acaccatata
    33961 taacatctgt acttggaggt tctgtagcat tatctggtgc attagcaata ataagacagc
    34021 atggtctaaa attatttgga gcaccatttg gaggatattg tgcagacaaa ttaaaatctc
    34081 ctacaaaagt acttttacca atatatgtat ttggtatagc agtaatagtt ttattcctag
    34141 tattacctgc atcaactcca atgactatat tcatagcttt aacatttgta gtaggtatat
    34201 taggttacat gggaaaaggt atatattatg cagtacaaga tgaagtaaaa gttccagtta
    34261 aatattcagc aactactata ggtatagctg cagctttagg attttcacca gatgtattcc
    34321 aatttgcatt aataggacac tggatagata cttatggcaa taaaggttac acatatacat
    34381 ttatattcca aatagcaatt ctagttattg gaatactatc ttgtttatat atattaaagg
    34441 ttaaaaagag aagagcaagt aaattagaag cttaaatatt caatttggta attaagtata
    34501 tataaataca atacttattt tgatgaatgt gattgtataa ataacataaa aaatatcaat
    34561 agattaagtt ttataataat gtttagagaa taaagtataa aaatcaaaat gttatcaagt
    34621 tttctaggtt ttaacataaa gttacccctg aaggtgtaaa attgagtggc aattattatc
    34681 tattttctta cctacataaa cctatttaat ttaaattcta agagatttta aaaatagatg
    34741 gtagttgtac tcaattttaa acctcataat aaatacgaaa atttaaaatc taactagata
    34801 ctatttaatt atatattaat aaatatatat tgtactattt atataataat aatttcaggc
    34861 ttttcattat atgtattcca atttatattg tttgattatt attcaaacac acgtcgttaa
    34921 aaattttata aatttatgtt tagatatgat taatactctt gattttagaa ataaatattt
    34981 gtagattgta gattaaagat aatataaata aatagaaggt agatttttta gatatattaa
    35041 tataaagatt atataaaata ttgaaatata gctgtttaaa ttattgtcta taaatataaa
    35101 atgaatggtg gtgagttaag tgaccgattt agttaacacc attaatggaa ttgtttggtc
    35161 acccgtatta gttgcaatgt gtttgtgtat aggattatat ttttcattga aattaaaatt
    35221 ttttcaaata agagatgtaa aagaaatgtt tactctcttg cttgaaggag atagttctca
    35281 tgttggtata tcatcatttc aaggttttgc tacatctatg gctggaagaa tagggactgg
    35341 aaatatagct ggtgtagctg tagcaatagc aatgggaggt ccaggagcat tagtatggat
    35401 gtgtataatg gctacattag gttcagcaac tgcatttgta gaatctactc tagctcaaat
    35461 atataaagat gagcatgatg ggcagtatag aggaggacct ccatattatt ttgaaaaagg
    35521 tcttgggtgg aaaaagtgtt cagttttatt ttcaatagtt gcaataataa gttatacatt
    35581 ttttatgcct ggtacacaat caaacacttt atatctagca atgaatcaag catttggatt
    35641 ttcaaaaatg acaatagcag tagcatcttc tattgcatta gcgataataa tttttggagg
    35701 aattaaaagg attggtttat ttgctgaaaa agtagtacct ataatgggtg gagcatacct
    35761 tattatgaca ttagtaataa ttataacaaa tattagttta gttccagagg ctattgtaac
    35821 aatgtttaaa tctgcatttg gatttggttc agtttttgga ggaatgctag gttcagcaat
    35881 atcttgggga gtaaaaagag gtatatactc aaatgaagca ggagaaggtt ctgggactta
    35941 tggtgcggca gcagcagaag tgagtcatcc agccaaacaa ggattagttc aagcattttc
    36001 tgtttatgta gacacattat taatatgtat ggctacaggt attatgattg taataacagg
    36061 aatgtataat gttacaggtg tttgcacagc actgccaggt gtagaaccgg gaccacaata
    36121 tgtacagagt gcaataaata cattacatcc acaattaggt gatattttta tagcaatagt
    36181 tttattatta tttacattta caactttatt agccttctca tatatgatgg agactaatgt
    36241 atcttactta gtaactagaa ttaaagcaga aaaacatgaa aagtacataa ttaatgtctg
    36301 tagatttatg cttatagcat gtgcagcaat gtgttgtttt atagaaccac ttacaagctg
    36361 ggcattaggt gatatatgta taggtgtatt aacatatata aatttaattg ccatctttac
    36421 actaaaaaaa ccagctataa aggcatatta tgattatata agacagaaga agatgggaat
    36481 aaaaaggagt gatagaactt ttaaccctat agagttagat ataagaaatg ctgagttttg
    36541 ggaaaataga tacttaaaag aggataagca tgagttaaat taaagttata ataaaatata
    36601 aaaattacta aataaattta gtgggaggta agattggtat gaaagaaaga ataatacaag
    36661 tggctgattc aaagaaagaa aaaattctag ggctatgtca atctttatat gatgaaccag
    36721 aaattgcttt gcaagaatat aaatctgcta aaaaaatatc agaattttta agagaagagg
    36781 gttttgatgt tgaggaaaac ttggcaggta tggctacagc ttttaaagca accaagaaaa
    36841 atggagatgg tcctaaaata gcatttatag cagaatatga tgctcttcca ggcaatggac
    36901 atgcatgtgg acatcattta attgcatcta tgggagttgg agctggaata gctctttcaa
    36961 gtatactgga tacttacaag ggagaagtaa gtataattgg aacacctgct gaagaaactg
    37021 gagatggaaa gccttacttg atagaacatg gtgtatttga tggctatgat gcagcaatga
    37081 tgatacatcc aaattcaaaa acttgtgtaa cacctgaaat tatagcaata ggaggtctag
    37141 atttcatatt tactggaaaa gcatctcatg caggagctaa gccatataac ggaataaatg
    37201 ctttagatgc agtagtatta ctatataata atataaatgc actaagacaa caattagtag
    37261 atggaacgag aatacatggt attatactag aagcaggaac tgctgcaaat gtaatacctg
    37321 atatgggaaa agtaagatta gagataaggg caaaagaaca aaattacttt gatgaagtag
    37381 tagaaaaagt gaaaaattgt gctagaggtg ctgcaatagc cactggatgt gaacttgaat
    37441 tttatcactt tgaaccaact tgccaaggat taaatgaaaa taaagttttg gtagacatat
    37501 ttacaaagat tatggaggaa tttggaatat atgaagatga acaagtaatg ttaggttcaa
    37561 cagatatggg aaatttaagt caaataatgc catgtataca tcctttgatg aagttttctg
    37621 aaaatggaga agaactacat acaaaagaat ttttggaggc ttctataaat tcatatgcaa
    37681 aagatagagt aattgatggt ataaaaatat tagctttaac aggatttaat ttatttgaaa
    37741 atccagagtt attgaaaaaa atgaaggaag agtagaactt agactatttt taattatact
    37801 ctttaaaata agaagagtgt ctcaaaatga actttttagt ttatgagaca ctcttttttg
    37861 tatgaaatta aatatatttt tattatttta acaatgaaaa agaggcttat ctgtttttat
    37921 atgacctttt ttaacttgta gcacaaaata tcatgaatga ttacaattcg aatgataaaa
    37981 tcgattataa gatatatagg aggggtacat atggaaactt tagaagtaag aaatattgaa
    38041 ttgagtggta caaattatga gataggatac agactaggag aattggttgc taatatgcca
    38101 gagataatag aaggtcagat taataaatct aatctggtaa gcaaaaaaga agaaaaagag
    38161 atgatagagt tgtttgataa atattgtcca ggtcttaatg aagaattgca aggttttgct
    38221 gatgcaattc aagtgaattg taatcaaatt ttatattata ctatgactta tcttaaacca
    38281 ggttgtagtc aagttgcatt agcaccagaa ttaactgaaa atgggcatgt actatttgca
    38341 cgtaatttcg atttctcaca caatatggag gattttgttt tatgtaaaac taaggtaaat
    38401 ggaaaatatg cacatatagg tactaccgtt atgcaatttg gaagaggtga aggtatgaat
    38461 gaatgtggct taggtgtaag tcagtcatca tgtggtattc ctgtaggcaa ttcagatggt
    38521 ttaagaaaac cagcaattgt aggtttacag ttttgggctg ttattagata tttgttagaa
    38581 aattgcaaag atgtagatga agcattagaa tatttaaaag atatgccaat tgcatacaat
    38641 attaatttat tgcttgcaga taagtctgga aacattgcac ttgtggagac tttagatggg
    38701 aaaaaagaag taaatataat caatagttta gaaagtaaaa gagaatattt tttacactct
    38761 actaatcata cacatattga taaacttcat aaattagagc cacaatcaat gaaaaattct
    38821 attcatagat ataaattaat taaagaatat ataaataagt ctaaaaaaat tggagagaaa
    38881 gagttaatga atttattgtc ttcaaaatat ccaaatggct tatcttgtaa ttactataat
    38941 gatttttttg gaactttaaa aagtatagtt atggatttaa atataggaaa gtttaatatt
    39001 ttatggggtg gactagagaa taaatgggag agctactatt taaaaaatga cattaaatct
    39061 ataacacaga ggataaacat aaatattgaa aaagcaccat ctgacttttt tgatttcatt
    39121 gaataaaaaa tgtatttaat tattctaaat aattaggata tataaatcaa ctaaaagttt
    39181 ttatttatcg tattgaatta gttataatat tttagtatag taaaaaatag tgctatttta
    39241 catatttaaa gagtttaagt agaagagagg tgccatattg gattacttaa agtattttaa
    39301 aaaagctgta atgtatattg agaataattt acatgaaaat ataactgtaa atgatgtggc
    39361 aaaagaaact ggatattctt attatcattt aacacgctta tttaagtcta tgtttggtga
    39421 gagtgtagga agttatataa aaaaaagacg attagtaagt agtacaaaag aacttttata
    39481 tagtgataaa aaagtgatag atatagctat tggtagtgga tttgaatctt cggaagcatt
    39541 tagcagagca tttaaatcta tatacaaggt tagccctata gaatatagaa aaaatagaat
    39601 tgatgtattt gttggtaaaa agaaaaaatt agagttagat tttatgagac atcttgttgg
    39661 aaatataacg attaaaccag ttataaaaga aatagaaaaa ataaaggtaa taggtataaa
    39721 ggataaagta attttagaaa ataattcatt acctgacttg tgggagaaat tcagaaaagt
    39781 acatcatatt gtcccaaata ctttgccatc aaagagagtt tttggaatct gtgaagcaat
    39841 ctctgaaata cacctagtta gtgaaagtat ggaatttaat gagataatag gacttgaagt
    39901 taatagttat gatgtgattc caaattcatt tgtttcaaaa actattaagg aaggaaagta
    39961 tgctatattt actcatactg gtagcttaga taatttagac aaaacttatg aatatatatg
    40021 gggtacttgg tttttaagtt caaaagaaga acttgatata agagatgatt ttgaagtata
    40081 tgatgaacga tttttaggtc ctagtaatgt gaactcacaa attgatattt atatacctat
    40141 aaaataatct ataagaaaac taaaaaataa tttgattaag aaatagatgt tcgtaaa
//
LOCUS       DAEMGG010000024        39088 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEMGG010000024 DAEMGG010000000
VERSION     DAEMGG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.024"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            40..115
                     /locus_tag="KPK24_001207"
     tRNA            40..115
                     /locus_tag="KPK24_001207"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:73..75,aa:Val,seq:tac)
     gene            121..197
                     /locus_tag="KPK24_001208"
     tRNA            121..197
                     /locus_tag="KPK24_001208"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Asp,seq:gtc)
     gene            207..281
                     /locus_tag="KPK24_001209"
     tRNA            207..281
                     /locus_tag="KPK24_001209"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:239..241,aa:Thr,seq:tgt)
     gene            296..380
                     /locus_tag="KPK24_001210"
     tRNA            296..380
                     /locus_tag="KPK24_001210"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:330..332,aa:Tyr,seq:gta)
     gene            390..473
                     /locus_tag="KPK24_001211"
     tRNA            390..473
                     /locus_tag="KPK24_001211"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:424..426,aa:Leu,seq:tag)
     gene            498..572
                     /locus_tag="KPK24_001212"
     tRNA            498..572
                     /locus_tag="KPK24_001212"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:530..532,aa:Gly,seq:gcc)
     gene            597..673
                     /locus_tag="KPK24_001213"
     tRNA            597..673
                     /locus_tag="KPK24_001213"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:631..633,aa:Arg,seq:tct)
     gene            683..758
                     /locus_tag="KPK24_001214"
     tRNA            683..758
                     /locus_tag="KPK24_001214"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:716..718,aa:Gln,seq:ttg)
     gene            767..842
                     /locus_tag="KPK24_001215"
     tRNA            767..842
                     /locus_tag="KPK24_001215"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:800..802,aa:Lys,seq:ttt)
     gene            845..933
                     /locus_tag="KPK24_001216"
     tRNA            845..933
                     /locus_tag="KPK24_001216"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:879..881,aa:Ser,seq:tga)
     gene            937..1012
                     /locus_tag="KPK24_001217"
     tRNA            937..1012
                     /locus_tag="KPK24_001217"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:Phe,seq:gaa)
     gene            1019..1092
                     /locus_tag="KPK24_001218"
     tRNA            1019..1092
                     /locus_tag="KPK24_001218"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Met,seq:cat)
     gene            1107..1183
                     /locus_tag="KPK24_001219"
     tRNA            1107..1183
                     /locus_tag="KPK24_001219"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1141..1143,aa:Ile,seq:cat)
     gene            1201..1277
                     /locus_tag="KPK24_001220"
     tRNA            1201..1277
                     /locus_tag="KPK24_001220"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1235..1237,aa:His,seq:gtg)
     gene            1286..1361
                     /locus_tag="KPK24_001221"
     tRNA            1286..1361
                     /locus_tag="KPK24_001221"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1319..1321,aa:Lys,seq:ttt)
     gene            1369..1442
                     /locus_tag="KPK24_001222"
     tRNA            1369..1442
                     /locus_tag="KPK24_001222"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1401..1403,aa:Cys,seq:gca)
     gene            1449..1525
                     /locus_tag="KPK24_001223"
     tRNA            1449..1525
                     /locus_tag="KPK24_001223"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1483..1485,aa:Arg,seq:acg)
     gene            1538..1613
                     /locus_tag="KPK24_001224"
     tRNA            1538..1613
                     /locus_tag="KPK24_001224"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1571..1573,aa:Val,seq:tac)
     gene            1689..2102
                     /locus_tag="KPK24_001225"
     CDS             1689..2102
                     /locus_tag="KPK24_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298912.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSTFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2411..5842
                     /locus_tag="KPK24_001226"
     CDS             2411..5842
                     /locus_tag="KPK24_001226"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG2298913.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLLAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            6084..8012
                     /locus_tag="KPK24_001227"
     CDS             6084..8012
                     /locus_tag="KPK24_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG2298914.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAITPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            8469..8933
                     /locus_tag="KPK24_001228"
     CDS             8469..8933
                     /locus_tag="KPK24_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG2298915.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8940..9434
                     /locus_tag="KPK24_001229"
     CDS             8940..9434
                     /locus_tag="KPK24_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG2298916.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            9450..10475
                     /locus_tag="KPK24_001230"
     CDS             9450..10475
                     /locus_tag="KPK24_001230"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG2298917.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLKAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            10462..12909
                     /locus_tag="KPK24_001231"
     CDS             10462..12909
                     /locus_tag="KPK24_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG2298918.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            13089..14462
                     /gene="radA"
                     /locus_tag="KPK24_001232"
     CDS             13089..14462
                     /gene="radA"
                     /locus_tag="KPK24_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG2298919.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSEKLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            14465..15535
                     /gene="disA"
                     /locus_tag="KPK24_001233"
     CDS             14465..15535
                     /gene="disA"
                     /locus_tag="KPK24_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG2298920.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAITNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            15624..16718
                     /locus_tag="KPK24_001234"
     CDS             15624..16718
                     /locus_tag="KPK24_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG2298921.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16847..19024
                     /locus_tag="KPK24_001235"
     CDS             16847..19024
                     /locus_tag="KPK24_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298922.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKIIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESMKANIEKNIDVYLKNIFSMVGEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGITIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNMPKWSVIVFSLLILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGAGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(19210..20055)
                     /locus_tag="KPK24_001236"
     CDS             complement(19210..20055)
                     /locus_tag="KPK24_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2298923.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            20272..21438
                     /locus_tag="KPK24_001237"
     CDS             20272..21438
                     /locus_tag="KPK24_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG2298924.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLTEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            21460..23106
                     /locus_tag="KPK24_001238"
     CDS             21460..23106
                     /locus_tag="KPK24_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG2298925.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNTESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            23203..24576
                     /locus_tag="KPK24_001239"
     CDS             23203..24576
                     /locus_tag="KPK24_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2298926.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            25021..25974
                     /locus_tag="KPK24_001240"
     CDS             25021..25974
                     /locus_tag="KPK24_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG2298927.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            26012..26974
                     /locus_tag="KPK24_001241"
     CDS             26012..26974
                     /locus_tag="KPK24_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG2298928.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            26999..27985
                     /locus_tag="KPK24_001242"
     CDS             26999..27985
                     /locus_tag="KPK24_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG2298929.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEVAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            27998..29044
                     /locus_tag="KPK24_001243"
     CDS             27998..29044
                     /locus_tag="KPK24_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG2298930.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETILGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            29211..30941
                     /gene="lpdA"
                     /locus_tag="KPK24_001244"
     CDS             29211..30941
                     /gene="lpdA"
                     /locus_tag="KPK24_001244"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG2298931.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDTV
                     KENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSMPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            31359..34307
                     /locus_tag="KPK24_001245"
     CDS             31359..34307
                     /locus_tag="KPK24_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2298932.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEVISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            34285..34749
                     /locus_tag="KPK24_001246"
     CDS             34285..34749
                     /locus_tag="KPK24_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2298933.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            34829..35131
                     /locus_tag="KPK24_001247"
     CDS             34829..35131
                     /locus_tag="KPK24_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2298934.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            35193..36557
                     /locus_tag="KPK24_001248"
     CDS             35193..36557
                     /locus_tag="KPK24_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG2298935.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            36592..37065
                     /locus_tag="KPK24_001249"
     CDS             36592..37065
                     /locus_tag="KPK24_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298936.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            37237..37887
                     /locus_tag="KPK24_001250"
     CDS             37237..37887
                     /locus_tag="KPK24_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG2298937.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTASDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            38061..38993
                     /locus_tag="KPK24_001251"
     CDS             38061..38993
                     /locus_tag="KPK24_001251"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG2298938.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
BASE COUNT        15747 a         4314 c         7353 g        11674 t
ORIGIN      
        1 agctgtgagg gttcgatccc cttcacccgc tccaaaaatg ggaccatagc tcagctggga
       61 gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc caccatttgc
      121 ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg agggttcgaa
      181 ccccttccag gtcgccaaaa taaaacgctg gtgtggctca acggtagagc agctgacttg
      241 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      301 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      361 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      421 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      481 ttaattttaa taaacgagcg ggaatagttc agtggtagag cgcaaccttg ccaaggttga
      541 agtcgcgagt tcgaatctcg tttcccgctc caaattttgt gtttatattg taatgtgggt
      601 gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc
      661 tgtgcgctca ccacatatat attggggatt agccaagtcg gtaaggcaca tgactttgac
      721 tcatgtatgc gtaggttcga gtcctgcatc cccagccaaa tagtatggtc tattagctca
      781 gtcggtagag cacctgactt ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac
      841 caatggagag gtgtccgagt ggtttaagga gctggtcttg aaaaccagtg acttcgaaag
      901 gggccgtggg ttcgaatccc accctctccg ccaaatgccc agatagctca gtcggtagag
      961 caggggactg aaaatccccg tgtcggtggt tcgattccgc ctctgggcac caaaatgtgg
     1021 cggtatagct tagttggcta gagcgttcgg ttcatacccg aaaggtcata ggttcgactc
     1081 ctattaccgc tactaaaatt taatgtgggc cattagctca gttggttaga gcgcccggct
     1141 cataaccggt aggtctgggg ttcgagtccc tgatggccca ccacattaaa ttaacttatg
     1201 gtgggtatag ctcagttggt tagagcgcca gattgtggct ctggaggtcg tgagttcgac
     1261 tctcattatc caccccaaat tatatggtct attagctcag tcggtagagc acctgacttt
     1321 taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc aaaaaggcgg cgacatagcc
     1381 aagtggtaag gcagtggact gcaactcctt gatccccagt tcgaatctgg gtgtcgcctc
     1441 caataaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1501 ggttcgagtc cctttgggcg caccatataa atagcgaggg attatagctc agctgggaga
     1561 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1621 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1681 taagaaactt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1741 ttatagataa aaatagtaca tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1801 aagatgtaat agcatatcag ttaagtatag atttaaatta cttagattct cttataaatg
     1861 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttat
     1921 ctgatgaact tgcactggat ttagaagagc taatgccaaa atatacaata ataaatgcta
     1981 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     2041 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     2101 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     2161 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtaggaa ttttcacata
     2221 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2281 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2341 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2401 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2461 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2521 ggaagataaa tatggattgt ttagaacaaa agcagatgaa tcatatttga taggagaagg
     2581 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2641 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2701 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2761 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2821 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggcta
     2881 tcctgttatg ataaaagcat ctaatggtgg tggcggtaga ggtatgagaa ttgtacatcg
     2941 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     3001 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     3061 attaggagat aactatggaa atatagttca cttatatgaa agagattgtt ctgttcaaag
     3121 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3181 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcaggaac
     3241 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3301 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagtgc aaagtcaaat
     3361 actaatagcc caaggttaca gtttagatag tgaagaaatt aatataaaat cacaagatga
     3421 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3481 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3541 actagacgga ggaaatggat ttacaggggc aaacataagt cctcactatg atagtttatt
     3601 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3661 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3721 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3781 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3841 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3901 aagaatggat aaggatggct caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3961 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttattattaa cagatacaac
     4021 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     4081 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     4141 agctacttat gatgttgcat atagattctt aaaagaatct ccttggcgta gattacaaaa
     4201 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4261 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4321 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaagccatc
     4381 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4441 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4501 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4561 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4621 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcat cagaagctgg
     4681 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4741 taatgctata gtagaggcac ttaaaaatac agagagagat acaggtatag atttatttgg
     4801 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4861 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4921 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4981 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     5041 agttgtagga gatttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     5101 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     5161 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5221 agctataaca gtaagaccag gttcattatt actagcagaa gattttgatg aaatagctaa
     5281 atatctaaat gaaaaatatg atataaatgc taatataaga aatgttataa gttatgcttt
     5341 atatccaaaa gtatatgaag attatatcaa acatttacaa cattacaatg atatatctaa
     5401 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5461 agaagaaggt aaggtattaa caataagatt agttgaaatc ggtgaagtta aggaaaatgg
     5521 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5581 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5641 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5701 agcaaatcaa ccattaatcg tcatagaggc tatgaagatg gaaactataa tagttgctaa
     5761 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5821 attattaatg ataatgaagt aattgaatta aaataaatta aaaagcactc tgaaatgttt
     5881 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5941 ggacaatcac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     6001 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     6061 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     6121 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6181 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6241 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6301 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcggcaata atagtaatag
     6361 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6421 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6481 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6541 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6601 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6661 accaaatatt agataaatat tttaatggag aagaactaac tacggaagag atacaaaaag
     6721 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6781 ataatatagg cacaaaagaa atattagata caatatcaag ttatttagaa cctatcttta
     6841 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6901 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6961 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     7021 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gtcaattctc
     7081 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     7141 ttcctaaacc acaaatatat tatgcaataa ctcctaagaa taaaggtgat gaagaaaaag
     7201 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7261 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7321 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7381 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7441 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7501 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtc
     7561 taaaagactc tatgcaaaaa ggtatattag caggttatcc tgttacaaac ataaaagcta
     7621 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7681 caagtgcagc ctttaaaaaa ggcatggaag aagcgcaccc aatactacta gaacctataa
     7741 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgttatgggt gatataaata
     7801 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7861 cagaagctcc acaggcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7921 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7981 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa taataaataa
     8041 ttaatagata ctattaaaaa tatttttagg gttttaacta ggtataagtt tatgttagat
     8101 aaacttatac ctagttttta tatatttttg atggataaaa aaatatataa tactttctta
     8161 aacgttttaa taaaaagaaa gtattttttt attattatct ttacaaatgt ttagcaataa
     8221 agatgagaag gtatatttaa aatataaagt atttaaagtt ctgaaatcta atcataaagc
     8281 aaaaaattag catttggtta tcaataaata taataaataa aaactcataa ttaatgaaag
     8341 aataaatcca tatctaaatt tctataaaaa atttaaattt atacaattta gggctttaaa
     8401 aatttaaata ataggtgtaa aataatagtc aaagaaagtc aaaaataaaa aggtgtgtgg
     8461 tgatattaat ggcaactatg acagatataa ttgaaaaatt tattaaagat ttgatggaag
     8521 aagataatag catacaaatt caaagaaatg aactagcaaa cttgtttagt tgtgcaccat
     8581 cacaaataaa ttatgtctta acaacaagat ttacaataga tagaggatat tacattgaaa
     8641 gtaaaaaagg tggcggagga tatgttcaaa tagaaaaaat acgaaaaagt aaagatggac
     8701 atattagaga actactaaat gaaaaaatag gaagtcagat atcctataaa aaagcaaagg
     8761 aattattaga aggtttaaaa gagtcggatt tgattaacga aagagaatta aaattaatat
     8821 tatatgcaat agatgataaa tcattgtgta tgccaatata tgaattaaaa gagaaggtaa
     8881 ggtctaatat tttaaagaat ataataatag gattatttag tatagaggag tgataattta
     8941 tgctatgtca aaaatgcaat aagaataaag catctgtata ttataataag atagtcaatg
     9001 gagaaaaaac agagatgtat ttatgtagtg agtgcgcaaa agagaataca gaaatgaatt
     9061 ttaatttaga tatgcctttt tcaatgatgg atatattttc caatttaggt tttcaaccta
     9121 aaaaagaact tgaggaaaag ctagtttgtc ctaaatgtaa tacaacatat agtgagttta
     9181 agaataatgg aagatttgga tgtagtgaat gttataatgc atttagtagt caagtaaatc
     9241 caatgcttca aaatatacat gggcatatag aacatacagg aaaagcacct aaaaaatctt
     9301 tttataagat aagtgtagaa aatgagatta aagaactaaa agaagattta gatagagcta
     9361 taaaaaatga agagtatgaa ttagcagctc aatttagaga taaaattaaa tatttaaaag
     9421 gttctattga ctaagattgg agtgatatta tgaaagagaa catagtcatg aaatcaagag
     9481 ttaggctagc tagaaattta aataactatc cctttccaaa taaattagat aaagaatgtg
     9541 ccatggaaat aatagaaaaa gtaaaaaatg cgtttattaa ttctaactta gaacaaaaag
     9601 aagaatttga tttctataaa attgaagatt tagaccaatc taaaaaaatg cttatggttg
     9661 aagaacatat aataagccca gatttagcag aaaatgataa aagtgcagtt atagtaaaaa
     9721 aagataaaac tataagcata atgataaatg aagaagacca tataaggata caaactatat
     9781 gtgatgactt aaacttagaa tatgcatata gtgtagcaaa tgaaatagat gatttgttag
     9841 aatcaagttt agaatatgca tttaacacaa agctaggtta tctaacatct tgtccaacta
     9901 acacaggaac aggaatgaga gcttctgtaa tgatgcattt accagcatta agtcagctag
     9961 gttatatgga tgaactttat aaaatttcat cacaaatagg tatagcaatt agagggatat
    10021 atggagaacg aacagaagca ttaggaaata tatatcaaat atctaatcaa cttacacttg
    10081 gaagaacaga gtctaatatt atagaaaatg taagtggact tacaaaagat gcaatttcaa
    10141 aagaaataaa agctagagaa atattgcaaa aaaaattagg tataaaacta gaagataaaa
    10201 tatttagatc gatagggaca cttgaaaatt caagagtaat gagtagcgca gaggcaatga
    10261 gccatctatc aaatataaaa atgggaatag aaatgaacta tatagataaa ctagatttaa
    10321 aggctataga acaacttatg ataggtatac aaccagcaca tcagtcaata atgtataaga
    10381 gtgatgatgt tgagaataga gatataaata gagcaacata tattagagaa actttggaaa
    10441 aattaagagg agggatgaat tgtgaacttc aatagattta cacaaagagc taaaaaggca
    10501 attgatttag cgtttgaatc tgctaaaagt ttaggacaca atattgtcgg aagtgaacat
    10561 atacttttag gtcttttaag agaagaagaa ggtatagccg ctaaagttct aagtaaagtt
    10621 ggatttacag aagcttattt agaaggcaaa atagttgata tggaaggtaa gggagaagaa
    10681 atctcagaag acatagtatt aagtccaagg agtaaacaaa tacttgaatt atcaggtatg
    10741 tttgcaaata aattaaaaac aaattatatt gggactgagc atatattatt agcaattatt
    10801 caagaaggtg aaggaatagc taataagatt ttaaattatg ctggagtaaa tgataggact
    10861 ttagcccaat taacaattga tatgatgggt attagtgata aaaatcaata caaagcagaa
    10921 aatagttaca cgggcaacca gaatcaagca gaatctaaag tacttgataa gtatggaaga
    10981 aatcttacac tatatgcaaa acagaataaa atagaccctg taataggtag agaaaaagaa
    11041 atacaaagag taatacaaat attaagtaga agaactaaaa acaatccagt gttgatagga
    11101 gacccaggcg tagggaaaac agccatagct gaaggtctgg ctacaaatat agccttagga
    11161 aatgttccag aaacactaaa aaacaaaact ttatactctt tagagatggg ttcattattg
    11221 gctggggcta agtatagggg agaatttgaa gaaagaatta aagaagttgt agatgaagtt
    11281 gttaaaaatg gaaacataat cttatttata gatgagatgc atactataat aggggctggg
    11341 tctactggag aaggctctat agatgcttca aatatattaa agccagcctt agcaagagga
    11401 gaaatacaag ttataggtgc aactacaatt gatgaatata gaaagcatgt tgaaaaagat
    11461 tctgctctag aaagaagatt ccaaccagtt atggtagatg aaccaactaa agaggattca
    11521 ataaaaatat tagaaggatt aagagataaa tatgaagctc atcataaagt taaaataact
    11581 gatgatgcta taaaggcagc tgtagaatta tcaactagat atatatcaga cagatatctt
    11641 ccagataaag caatagactt aattgatgaa gctgcatcaa aagtaagatt aaaagaaaat
    11701 acacctcctt cagaaataaa aaaattagaa ctagaaatag aaaatataga taaagaaaaa
    11761 gaagaagcag taagatgtca agactttgag aaagcagcca aaataagaga tgaacaaggt
    11821 ctacttaaaa aacaattaga agatgttaga gaaagatgga acaagtcatc taaacattca
    11881 gatttagttg atggagaagt tattgctgaa gtagtgggct tatggacagg aataccagtt
    11941 aataaaattc ttgaagaaga ggctgataga cttttgagac ttgaggaaat actgcacaat
    12001 agagttatag gtcaagaaca agcagtaaaa tctatctcaa aggcaatcag aaggtcaaga
    12061 gcaggtctta aagaccctaa tagaccaatt ggttcatttt tatttttagg tccaacagga
    12121 gtaggtaaaa cagagttatc taaggcatta gcagaagtac aatttggaga tgaaaatcaa
    12181 ataattagaa tagatatgtc tgaatatatg gaaaaacatg ctgtgtcaag aatgataggt
    12241 tcacctccag gttatgtagg tcatgatgaa ggtggtcaat taactgaaaa agtaagaaga
    12301 aatccatatt cagttatatt atttgatgag atagaaaaag ctcatccaga tgtatttaat
    12361 attctgttac aaattctaga tgatggtaga ctaactgatt caaaaggaag aactgtagat
    12421 tttaagaata ctattgtaat aatgacatca aacgttggag catctacaat tggtagacaa
    12481 aaaactttag gatttagtat agctaaagga gacgaagaag aaaaatctca atatgaaaaa
    12541 atgaaagaaa atataatggg tgaattaaaa caaagattca gaccagagtt tttaaacaga
    12601 atagatgata taattgtttt ccactcatta aatgaaaatc atatatcaaa aatagtactt
    12661 ttaatggcag cgaaactaca agagagatta aaagaaatgg atataaaatt agaaatgagt
    12721 gatgaggctg ttaaattaat ttctaaatct ggatttgact tagaatatgg agcaagacct
    12781 cttaaaagag ctttacaaaa agagttggaa gatgagctat ctgaagcaat cttaaaaggt
    12841 aatgtgaaaa agggaagtaa tgtagtagca aaagtaaaag atgaaaaaat agtttttgaa
    12901 actaaataat ttaatagagt tgcattttaa aatattgcaa atataagttt tataatctaa
    12961 acaaaatttt aagggcaatg aatctataat aattcattgt ccttaattat tttatttaat
    13021 ctttacatat tgtctttcag atatgctaaa attatagtat agaataaaaa attaaggatg
    13081 ataaaagtat ggcaaaaata aaaacaaaat atgtatgtca atcttgtgga tatgaaacag
    13141 ctaagtggct tggtaaatgt ccagaatgta caaaatggaa tacatttgta gaagaaatag
    13201 accaaaaaag caccaaaaaa gaggtcttta taatagataa atcttcatct aaaccagtca
    13261 gtataaactc aatagaaagt aaagaagaag agaggtttac aacagatata aacgaattag
    13321 atagagtgct tggaggaggt atagttaaag gctctttagt gcttgttggt ggtgaccctg
    13381 gtataggtaa atcaacactt ttaatccaag tatctagtaa tgttgccaat ttaggaaaaa
    13441 ctgttttata tataactgga gaagaatcag aatctcaaat aaaaatgaga gctaaaagat
    13501 taggtattaa ctcagaaaaa ttatatatat ttgctgaaaa taatttaagt ataattgaat
    13561 cttatttaga aagtgtaaat ccagaattaa taattctaga ctcaattcaa actgtcttca
    13621 gtccagaaat atcctcggca ccaggaactg tcagccaaat taaagaaggt acttcaaaat
    13681 tcatgaaaat ttctaaaaaa atgggaattt caacgttcat agttgggcat gttacaaaag
    13741 aaggttcact agcaggacca aaattattag agcatatggt tgatactgtt ctatattttg
    13801 aaggagaaag atataatact tatagattag tgagagctgt taaaaatcga tttggctcaa
    13861 ctaatgaact tggagtattt gaaatgaggg atttaggatt agtagaactt gacaatccat
    13921 ctaaaatttt aatctctgaa aaaccaaaag atgtagcagg ttcagtaata atatctactg
    13981 tagaaggtac gagaccaatg ttacttgaat tgcaagcatt agtttctcct acaagttttg
    14041 gtataccaaa aagaacatct acaggagtcg attacaatag agtaggtatg ttattagcag
    14101 tattagaaaa acgtgttggt ttgcaaatac aaaatcaaga tgtctatata aacatagttg
    14161 gaggaataaa gataaatgaa ccttcaattg atttagggat agccatttct gtagcatcta
    14221 gttttagaaa tataccgata gatgaagata tagctgtcac aggagaagtt ggacttacag
    14281 gagaagtcag agcagtaagc tttatagaaa agagaatagc tgagtgtaaa aaacttggat
    14341 ttaaaaagat agtagttcct agaagtaatt atgatgttgt aaaagagacc aaaggcatag
    14401 aaatatggcc agtggataac ttaagacaag ctataaatat agtacttggg aggaatcaat
    14461 aaatatggag aattttctag ataataaaaa tatgctatat gcattaaaaa tgatatctcc
    14521 tggaactcca cttagattag gtttaaacaa tgtactaaga gctaagactg gtggactaat
    14581 tgtaattgca acaaacgaag atgtaatgaa aatagtagat ggaggatttg ctataaatgc
    14641 agaatattca ccatcatatc tatatgaatt agctaaaatg gatggagcta tagttttaag
    14701 tggtgatgta aagaaaatat tatttgctaa tgcacaactt atacctgact attttataga
    14761 aacatcagag acgggaacaa gacatagaac agcagaaaga gtagcaaaac aaactggtgc
    14821 tatagtcata ggaatttcac aaagaagaaa tgttataaca gtttatagag gaaatgagaa
    14881 gtatgtagtc gaagatatat ctaagatatt tactaaggca aatcaggcta tacaaactct
    14941 ggaaaaatat aagacagtat tggaccaagc tataacaaat ttaaatgcct tagagtttaa
    15001 tgatttggta actatttatg atgttgcatt agtcatgcaa aagatggaaa tggtaatgag
    15061 agttacaagt ataattgaaa aatatgtgat agaattgggt gatgaaggaa ctttagtaag
    15121 tatgcaatta gaagaattaa tgggtacaac cagaatagac cagaaattaa tattcaaaga
    15181 ttataataaa gaaaacacag aaataaaaga acttatgaaa aaggtcaaaa atttaaattc
    15241 agaagaacta atagaattgg tcaatatggc aaaactatta gggtatagtg gtttttcaga
    15301 aagtatggat atgcctataa aaacaagagg ttataggatt cttagtaaaa tacatagact
    15361 accaacagcc ataatagaaa acttagtaaa ttattttgaa aactttcaac aaattttaga
    15421 tgcatctatt gaagaattag atgaggttga aggaataggt gaaataagag caacatatat
    15481 aaaaaatgga ctcataaaaa tgaaacaatt agtcttatta gatagacaca tatgatgaat
    15541 tttaaatgaa ttttactact tttttcttga atataatata cactttggtc tataatagat
    15601 aagtataact agggggtgaa gcattgataa ggaaagtaac aagaatattg tttattttac
    15661 ttggatttac aataggtata acaacctatt taacattaat gaaagatttt gaaatattaa
    15721 catttggaaa agaaacatat ggttatatcg cagctgtagt agcaggtata ataatagcca
    15781 ttttaggata tcttatagaa ccatgggttg taaataaagt aaaagaaatt gctaaaatag
    15841 tggataaaga attgtcaaaa tatcctcaga ctgatatatt gttagggtct atgggtctta
    15901 tagtaggatt tgttattgcg tatttattaa gtggattagt aaatagaata ccaatagtag
    15961 gtggaatact atccttacta ttatatttat tcctaggata tcttggaatg aaagttgcgc
    16021 ttaaaagtaa aaatgattta tttaatattg gaaaactagg aagattagca aatcctatta
    16081 aggataaaga taaggataaa gaaaataaaa aagaagtaaa agccattcct ccaaaagtat
    16141 tagatacaag tgtaataatt gatggaagaa tagctgatat ttgtaagact gggtttatag
    16201 aaggaaaact tattattcct gcttttgttt tggaggaact aagacatatt gcagattctt
    16261 cagatgattt aaaaagagta agaggtagaa gaggtctgga tatattaaat ataattcaga
    16321 aagaattaaa tatagaagtt gaaataagtg agagagactt tgatgatata gcagaagttg
    16381 atagtaaatt attaaagctt gctcaggttt taaatggaaa agttgttaca aatgattata
    16441 acttaaataa agttgctcaa ttccaaggtg ttgaagtatt aaatataaat gagttagcta
    16501 atgctataaa accagttgct ataccaggtg aagatatggt tgttcaagtg gttaaagaag
    16561 gaaaagaggc tcaacaaggt gttgcatatc tagatgatgg aactatgata gttgttgatg
    16621 gaggaagaaa atatatgaat gagactataa aagtactcgt tacttctgtt cttcaaactc
    16681 ctgcaggtag aatgatattt gcaaaaccta aaaattaata tgttcaaaga aacctataat
    16741 ttgggctgtt tattttaaat agatggtcca aatttattta tttataacga taatgtttaa
    16801 tgtattctta aagatgggga tttaaatcag aaagcaggga gaatttatga gaaaaaagat
    16861 tatatctttc ttagcaacat ttatcataat attaacatca gcaagtcagt atagttttgc
    16921 agatgatata tcaacacgtg gaaaaataat atttatagat atgaatagaa ctagtatgag
    16981 taatatgtta agaataaaat cattaagaga agaactagac aatagagggt atattggctt
    17041 gatgaatata agaggcgata aaggaagtga tgatagaagg tcatatgcta gtatgggtgc
    17101 tggtggtaga gctaatgtgg ctaatgaaga agatattaat tttgaaagct cgagtaagga
    17161 tagaaatata gtttttgaat cagctacagg aaaaagtgca aaaggtataa ataatttaac
    17221 tataaataag tctataaatg aaaatttaaa ctttggagag tatggttctg tattagggtc
    17281 attaggacaa agtttatctg ataatggatt aaaagcatct gtattgggaa attcagatat
    17341 aattgaaaat ggccaactta taaaaaacag aaacttatgt ctaactgcta tggatgaata
    17401 tggaagaata cctaatggaa atgtagatac tataaataaa aaagacttat ctatgcctta
    17461 tggaatatca actgattatg ataaattgat tgtagagacc aaagaagctt acaaaaataa
    17521 tgatgtggtt tttgtggagc ttggagatac atatagatta gatttatata aaccaaattt
    17581 aaatgaaaaa acatatgaga gtatgaaagc taacatagaa aaaaatatag atgtatattt
    17641 aaaaaatata ttttctatgg ttggagaaaa tgacactgtc tacatagcaa gtgcatttcc
    17701 aagtgattta gattataaaa ataaaagaag actctctcca ataataaaat taaatggaga
    17761 agggaaaggt atattatcat cttcaactac tagaagagaa ggtattgtaa caaatttaga
    17821 tgttggggtt gaaatattag acaactttaa tataaaaaat caaaatatgg ttggaagaaa
    17881 atatgaactt attaacagag atgataataa agaatttttg atggatgaat atcaaaagat
    17941 agtatctatt tcaagcataa ggtctacaat actgaatggt tttgtaagca tagttttctt
    18001 gtcatggata gttgctatga ttgcaatttt atttagaaag catatatcaa aaaattataa
    18061 agaaacaaca ttttttatat taaaggaact tttaaaaata ggaatagtaa tgccattgtc
    18121 atttatgata acaccaataa tgaatttcaa aacaccatta gcaatatctt taggaataat
    18181 tataataaca cttactatat atcttataag taaagtttta ataaagaacg atttaaaaaa
    18241 catgctgttt tttactggaa taaccatagt aattatggtt attgatgctg gatttggctc
    18301 atatttaatg aaaagcaatg ttatgagtta tgactgtata attggagcta gatattatgg
    18361 agtaggaaat gagtatcaag gtgtggcaat tggtagtgca atatttacat ttgcaatttt
    18421 acttacatat aaaaatatgc caaagtggtc tgtaatagtt ttttctttgc ttatcttaat
    18481 tacaagtgca tctccaatta tgggagcaaa tgttggaagt gctatttctg aatgtgttgc
    18541 atttttacta tttatactat tgatatataa tgtaaagata gatttcaaaa aaatcgtatt
    18601 attaggtata gcagtagtat ttgtattagg agtttttgta gcaatagata tgattttggg
    18661 aagtaattca catttaggca tgtttgtaaa agaaatttac tttaatggag caggagaaat
    18721 aatacaaaca tttagtagaa agatagagat gaatttaaag ctagctcaaa ccagtgcatg
    18781 ggtaaatata ctgttaactg gaataggtgt aattttagtt cttatgatta atcagattag
    18841 atactttaag caactgatgg atgagtatcc aattgtattt aaaggattta tagcatcaat
    18901 agcaggatgt ttagtaactc ttttagtgaa tgactctggg attgtgtcat cagctacagc
    18961 ctttatttat gtaattgtac ctatgataac attaagtata aatctcacag cattaaaaga
    19021 ataagtactg tatgaataat atgtaaatat taatacatta atttaacttc tttatttaca
    19081 aaataaagaa gaaagtttat aaaaaataaa ttataataaa aaagagttta tctcaaaatt
    19141 gattattcta atcaaatttt aagataaact ttttttatta taaagataaa taacatataa
    19201 cctcatattt taggtagatt tttttcttat atgtttagga gtgcatttaa atttttcctt
    19261 aaaaattctg taaaataatt ttgtattagt aaatccatga gcttctaata tactgcataa
    19321 ggtatcattt gtgttaatta aatctttata aatatgagaa atacgcacat catttaaata
    19381 ctctaagtaa gtttgtccca tatactgttt aaattttcta caaaaatatt ctggttgtaa
    19441 gtgtgctacc ttagcgattt tgttaataga aataggcatt ttgtaattaa tctttgtata
    19501 ttctaaaact ggttcaaatt ttgacaaatc aagtactgtt tttttcatat ttttattacc
    19561 tacagatatt ttaaagtttg tatataattc aaataaaagt tcaaataata agcttgtaaa
    19621 gagcagatta gctgcaggag gagaactaag tttaatttct ctcatgtttt ctaatataag
    19681 tttaacttta cttgaatttt ttttatagtt attgtcattg gcattaacct taaagtcaaa
    19741 atagcaattt ttaaaatcag ggatatattt gtttagaaaa gacaatggta cttgcaataa
    19801 tatagaggta ttttgataaa tacatctagt tgagtgaata attccagaat taatgaacat
    19861 acactcatta gcttttaaat tataagtgta accctccatg ttaatttgta aatcaccaga
    19921 agttatataa attatttcta atgagttatg ccaatgattt gctacataac taccactatc
    19981 atcaaatagt ttgaaattaa catctaaatt atcaataggt tcaattattt catgtgaaaa
    20041 attatttctg ttcataataa tatcctcctt ataattttaa attataaata gtaaatttat
    20101 aatgttcaat attaaaaaga taatatagac taataaatta gataataaac atacttaaga
    20161 aaacggataa aatcaaatat aataactgta tattaaaata acttaaaaaa attattttat
    20221 tataagtgca aaatatacct atttaaataa gataagaggg gaggatttta tgtgtatata
    20281 aaaggtgtaa atttaggagg ttggttagtt ttagaaaaat ggatgacctc aagtttattt
    20341 gaaggcacag aagctgaaga tgaatactat ttaccaagac agctttcaag agaggcatat
    20401 gaatctagaa ttaagactca tcgtagtgaa tacatcacag agagagattt tgcaacgatt
    20461 aaatctatgg gatttaattc agttcgtata ccagtaccat actttatttt tggagattgt
    20521 gaaccattca taggatgtgt taaagaactt gataaagcat ttgcttgggc agataagtat
    20581 gggcttagta tattgataga cttacataca gttccaggaa gccaaaatgg atttgataat
    20641 ggaggaatat caggtatatg tagctggtca caaaatccag aatatgtagc gtttacatta
    20701 aatgtattag aaagacttgc aaaacgttat gggatgaggc atgaattgta tggaatccaa
    20761 attttaaatg agcctataac agaaagaatg tggaacataa tgaatgttcc aaatcggttt
    20821 aaggctgtag ataaggagat ggcaaggggg agtaaaccta attcattaga gtttttacgt
    20881 gatttttata taaaagcata taaggtaatg cgtccttata tgagagagga aaacgttata
    20941 gtgtttcatg atgcatttga attaaaagca tggaaagact ttatgagaga ggaagaattt
    21001 aaaaatgttg ttctagatac acaccaatat ttgatgttaa ctgaagcaga tggatgtgaa
    21061 caaagtattg atagctatct caaatatatt agagaaaatt atgctaaaga tattttacaa
    21121 atgcaaaaat attttcctgt tatatgtggg gagtggagct tatttaattc atatgcttgt
    21181 ggtatagata caaatggagg tcaatcacct ctaaatggga ttgaatcaaa tatagataaa
    21241 ttatcaaaag acgataaaag agaattatat aggaagatag caaaagcaca gcttgatgca
    21301 tggagaaatg gaagtggtca ctactattgg aattataagt tattactaga tacagttaat
    21361 gaagaaggtt ggataggatg ggatagttgg gacctaggca aatgtgtagc tcaagaatgg
    21421 tatcctatag aatattaaac taaaattgga ggaaaagata tgattaaaaa ccctatactt
    21481 ccaggtttta atccagatcc ttgtatttgt agaaagggag atgattatta tcttgtagtt
    21541 tcatcttttg aatggtttcc aggtatacct gtttatcact ctaaagactt aaaaaattgg
    21601 gagttatata ctcatatttt gacagatgaa acaaaaatag atttgaagaa gcttccttca
    21661 tctaaaggaa tttgggcacc ctgtttgact tattgtgaag aagaagattt attttatata
    21721 gtttatggaa ttatgaactc tatgaatgca agatattttg atgttgataa ttatttaatt
    21781 acatcaaaag atataaaggg agagtggagt gaaccagtat atctacactc atcaggtttt
    21841 gatgcgtcta tttttcatga tgatgatggt aaaaagtgga ttgcatcatt agactgggag
    21901 acaagagaag gatatgaaaa acctggtgtt atatgtttag tggaatactg tacaaaaaag
    21961 aaagaaatag taggatatcc aaagagaata tggtcagggg gaactgatag aggttgtatt
    22021 gaagcaccac atataacaaa gcgtggagat tactattata taatgtgtgc agaaggtgga
    22081 actggatatg gtcatagtgt aactatggga agggctaaaa atatatgggg accttatgaa
    22141 aaagattcaa tgaatccaat tgtaacttca attccaggcg atttttatga aagacatgac
    22201 cctgaccatc taaagcctaa atactataac acagaatcaa aacttcaaaa atctggtcat
    22261 ggaagctata tagaaacaac atcaggtgaa gtatatttag ttcatctaac atctagacca
    22321 tttgtaccag agttaaggtg tactttaggt agagaaacag ctattcaaaa aatgaaatgg
    22381 acaaaggata attggcttcg aatggaagat gaaagtaatt tggcaaaaga atacgtatca
    22441 gaaagtaaac tagaagaaca tttagtttca tctataccaa gttttgatga ctttgattca
    22501 aatgaattag gtttacagta ctatgctcct aggatttcac cattatcatt tgcagatgtt
    22561 aagtctagac caggatatgt aaggattaga ggacaagaat caagaacatc attaaataaa
    22621 gtgagcatat tggcaagaaa attaacttca gtatatgcaa gaataaccac taagatggag
    22681 ttttacccag aggtacatca gcatagtgcg ggtttgataa tgtattatga taatatgaat
    22741 tatataaatt taagaaagta ttatagtgaa acattgggtc aaagtgcact atcaataata
    22801 catcttgaaa atggagagaa aacggaattt ttaaatacta ggataccaat aaaagatata
    22861 ccaatatatc ttcgtctgta tatacaaggt cgtaagtcat attttgaatg gagttatgat
    22921 gaaaaaaact accagagaat aggaaaggta tttgatacaa ctaagttttc agatgagtac
    22981 tgtaaatatg gagaatttac aggtacgttt ataggattaa cttgtgcaga tcgtgtaaag
    23041 cataaacact atgctgattt tgattttttt gaatatatag tagatgaatc aaaagatgtc
    23101 gattaataaa atcattacta aaattttcag agcaaaggta aatcttatgc tctgaaataa
    23161 gaaacattag aaggcaaatc ttacaaaaag gagtatggta ttatgaatac agaagttaat
    23221 gttaaagcaa ctacgattag atatgaaaag atttcatttt ttgaaagaat ttcttatggt
    23281 tgtggggatt tggggtgcaa tatcatatac tcagctatgt cagcattttt gctgttttat
    23341 tatactaatt atgcagatgt tagtgcagca gctgtaggta gtattatgct tgtatctagg
    23401 atactggatg ggtttagtga cttaacaatg ggaattatag tggatagaac taaatccaaa
    23461 tacggtaagg ctaggccttg gattttgaga atggcgattc cttttgctat agcagcagta
    23521 ttattatttt ctgttccatc aaatcttggt atcacttcta aattaattta tatttttatt
    23581 acatataact tagtttctac tgttatttat acagcaatca atgtacccta tgcaactcta
    23641 aattcactta taactcaaaa ccagtatgaa agaggagtat taagtatatt tagaatgata
    23701 ttagccactt gtggtacctt actaataaat ggacttacac tacctctagt tgaatacttt
    23761 ggaaataatt tatcagcatg gactaaaaca ttctttgtat ttggaatagc atctataatg
    23821 gtatttttta taacatttac tggaaccaag gaaagagtaa aagcagttaa gcaagatagg
    23881 aatgaagtaa taccatttaa aataggtatt aaatcgttat ttagaaataa gtactggata
    23941 caaataacat tatgtttagt ttgcatattt attgtatttg cgataaatgg tggttcatcc
    24001 gtatattatg caaagtttat tttgggagat gaaaaattat ttggtcctat aaatatggtt
    24061 tcaaatatat cccaaattat agctatgttt atggttgctc cttttataaa aaaatttgga
    24121 aaaagaaatg tattaatagt aggttcaata atattaatat cgtcaaatgt tatgtttata
    24181 atagttggac aaaactatat aggagtaata tttgcaagtg ctataaaggg aataggaagt
    24241 gctggaatag catcaacaat gtttgctatt gtttcagata ctattgaata tggagaatgg
    24301 aagactggat atagaactga ggggcttata aatagtgcat ctagttttgg gtttaaagtg
    24361 ggaaatggat taggttcagc aatattaggt gcagtattat caattggtgg ttatgtagga
    24421 acatcagcta ctcaaagtga tttagctata gtatcaataa aagtatgttt tatataccta
    24481 cctatattta taacaatatt acaaattatc ataatgtctt tttataagct tgataaagaa
    24541 tatagtacta tacttaatga attaaatact aaataatgaa aaagtttctt ataaagtctt
    24601 aagtttaatt aaggtctatt gtaaatgttc cctataaaaa ctattatata tttatgatta
    24661 ttgaagaatt tttagagaag cgaataataa aaatataaga agactttaaa tgatgaaatg
    24721 gttggttttt tagaaatgat ttaataagtt attgttattt aaagcattat aagtggacaa
    24781 ttaattttta atttatggag atgatattta acaattagaa gtaatatata atatgcacta
    24841 agaagcaatt tttcaactat ttatgaaggt ggtggtacaa gaaaagtatc accttttttt
    24901 tgttatacaa aaaaacagtt gactttttat atgaacccct tctataatgt tactgaaatt
    24961 aagtaaaatg attgaacaaa tgtaaagata attgaaaagc ttgattttgg aggaaagcaa
    25021 atgaaaaaaa ttggagactt aagattaatg atgaagtgtt gttcacttta ttacgaagat
    25081 aatttgaatc aacaggaaat agcaaatcaa ttgggaatat caagaccaac aatatcaaga
    25141 atattaaaag aagcttttga acaaggaata gtaaaaatac agatagttga tgttttgaaa
    25201 aatgattacc aaaaaataga gagaagtttg gagagaaaat ataaattaaa agaagtaata
    25261 gtagtagatg ataaacagga tgcactaaca caaaaacaag aactagcaag agctgtatct
    25321 gaatatttaa ctagagtagt aaaggaaaat gatataatag gtgtttcaat aggaacaaca
    25381 ctaaaagaaa taccaagata tgtggaaaaa agtaactgta aaaatgttac atttatacct
    25441 ctattaggag ggattggaga caatgaaata gatattcatg caaatcaaat agccgtaagt
    25501 ttagcgaggg cttttggagg tgattttaaa ttattacatg ctccagctgt tatgtctgac
    25561 ttgtctacaa aagagaaact ttgcaaagac gaaaaaatta aagaagtact agatttaata
    25621 gataaaacaa ctatagcaat tgtgggtata ggaaatccta tgagtttaaa ctcaacaata
    25681 atggcttctg gatacatgaa tgaagcagat atagaagatt taaaaaaata caactcaata
    25741 ggtgcaattt gtttacaagc ctttgataaa gaaggaaaaa cttctatact tgaatttaat
    25801 caaagagtat taggagtgaa actggaagat ttaaaaaaga taaaaagaac aataggtgtc
    25861 gcttcaggag atgaaaaaat agaggcaata aaggcatcat taaaagcgaa atttataaat
    25921 agtttagcaa ttaatcacag tttagcactt aaattattag aagattgtga ctaatagaaa
    25981 gatagataaa agttaacagg agggaatgta tatgtcaata tcaaaagaaa cattgcttga
    26041 aatgtataag agaatgaatc aggctagaaa atttgaagag aaagttagtt ggttttttgc
    26101 aagaggtatg gttcatggaa caactcactt atctgtaggt caagaagcgt caagtgtggc
    26161 ggctgtaatg gctttagaaa aaggagattt agtatcatta actcatagag gacatagtca
    26221 atttatagga atgggaatag acctaaataa aatgatggct gaacttatgg gtaaagaaac
    26281 tggattttgt aagggaaaag gaggctcaat gcatatagca gatattgagt caggtaactt
    26341 aggagcaaat ggagttgtag gtggaggtct tacaatagct ccaggagctg cacttacaca
    26401 acagtataaa aaaacaggca aaatagtttt atgtagtttt ggtgatggag catcaaatga
    26461 aggtactttt catgaaggaa taaatttatc ttcaatatgg aaattgccaa taatatttta
    26521 ctgtgagaat aatttatacg gtatgtcaac aagcataaaa cgtcatatga atatagaaag
    26581 tattgccact agagcagctt cttatggaat agaaggaata tcaatagatg gatataaccc
    26641 aatagaagta tacgaaacag tacaaaaagc tgctgagaag tgtagaagag gagaaggacc
    26701 tgtattaatt gaaagtagaa cttatagatg gttaggtcat tcaaaatcag atgcaaatgt
    26761 atatagaaca aaagaagaaa tagaatcatg gaaagcaaaa gaccctattg aatttttaaa
    26821 aaattatctt atagaaaata atctatctaa tgaagatgaa ttagataaga ttcaagaatt
    26881 tgcaaagcaa tcaatagaag atgcagtaga gtttgctcaa aatagcccaa atccaaaaat
    26941 agagtcttta ttagaggatg tatatgcaga ctaagaatgg taaaggagga aaataaaaat
    27001 gagtacaagg gaattaacat atgcacaagc aataaaggaa gcaatgtcag aagaaatgag
    27061 aagagatgaa aatgtaatat ttatgggaga agatatagga atatatggag gagcatttgg
    27121 tgtatcagtt ggtatgatag atgaatttgg accagaaaga gtaagggata ctcctatatc
    27181 agaagcagct atagcaggag cggcagcagg tgcagcagca actggcttaa gacctattat
    27241 ggaagtaatg tttatggact ttgtaacaat ttctatggat gcaattgtaa atcaagcggc
    27301 aaaaatgaga tatatgtttg gaggaaaagc acaagttcca atggttgtta gatgcccagg
    27361 aggttctgga actggttcag cagagcaaca ttcacaaagt ttagaagctt ggttttgtca
    27421 tgtaccagga gtaaaagtag ttgcaccttc tacaccagca gatgctaagg gattgttgaa
    27481 agcagctata agagataata atccagtcat atttgttgag aataaattgt tatacagaaa
    27541 aaaaggtttt gtgccagaag atgattatgt aattgaaatt ggaaaggcag atattaaaag
    27601 agagggtact gatgttacag taattactta tggaagaatg ttacaaagtg ttgaagaagt
    27661 agcagagact ctatcaaaag aaaatataaa tgttgaaatt atagatttaa gaactttata
    27721 tccattagat aaagagacta tagtaaagag tgtatgtaaa actgggagag ttttaatttg
    27781 ccatgaagca gctaagactg gtgggctagg tggagaaata tcagccctga taacagaaag
    27841 tgaatcattt gattatttag atgctccagt aaaaagaatt tgtggaaaag atgtgccaat
    27901 accatataat ccagagttag aaaaagcagt tgtcccaaga gttgatgaaa tagaagaagc
    27961 aataaaatct ttaattgtta gataaggagg gatagatatg gtggcaaata aaataaaagc
    28021 cacaccagca gctagaagtc aggcaagaaa agacaatata aaattagata ggctgattgg
    28081 aagtggagaa aatggaagga ttcatttagt agatgtgtta aattatttaa aagataataa
    28141 agcaaatact actcctttag ctagaagaat agcagaagat ttaaatatag atttagaaac
    28201 tattttaggt actggatata atggaaagat aagaaagtgt gatgtggaaa aattaactgc
    28261 aaaagaaact attgtatcaa ctaatacatc taaatctagt gagaaaaaag agctgaaaat
    28321 tgaaaatgaa aattcaagca tgtttaacac agtagaagga atatttgaaa agccaaatcc
    28381 aatgagagct actgtagcaa aacgaatgtc agaaagttat tttagtgctc ctgtatttac
    28441 attcaatata gaagtagatg ctacagaatt gaaggtactt agagcaaagt taatcgacac
    28501 agtaaaagaa tcaactggag taaaattaac tatgacagat ttaatagtaa tggcagtatc
    28561 taaaatactt ccaaatcatc aagccttaaa ttcagcatgg acagatgagg gtatatttag
    28621 atataaagat gtaaatatag ctattgcagt tggtttagat gaaggcttat atgttccggt
    28681 agtaaaaaat gcaaacaaaa agtctttaaa agaaatagca aaagaaagca aagaacttgc
    28741 ggaaaaagta aagacaggga aacttatgcc agcagaccaa gaaggtaata catttacaat
    28801 aagtaatgtt ggaatgtatg gtataactac atttacacct ataataaata tgccttcaag
    28861 tgcaatctta ggggtaggtg ctacacaaga taagtttgtt ccagtaaatg gagaggctaa
    28921 aataaaacct ataatgaact tatcattaac atcagaccat agagtaatag atggaacagt
    28981 agctgcaaaa ttcttaaaag atttaaaaga attgttagaa aatcctttat caatgcttgt
    29041 ataggttttg atattttgtt actgatataa atagtttgaa tagattatat tattataatt
    29101 gtttcagtaa atttaaagct tttatatatt aagaattatt tttaacagta actatatgtg
    29161 aatatgattt gcttaattaa aatataagaa aataaatagg aggagagcct atgtcagtag
    29221 aagtaataat gccaaaagca ggagtagcca tggaagaagg tactatagta tcttggctaa
    29281 agcaagaagg cgaagaagta aagattggag aacctatact agaaataaca acagataaag
    29341 ttaatatgga gatagagtca gaaggagaag gtacacttgc tgtaatcata cataaagagg
    29401 aaggtgaagt attaccagta tttacagtaa taggtgtaat agcagaaaaa ggggaaaatc
    29461 aagaggaagt aaaagctaaa tatttatctg gaaatgtttc aaaagaagat acagttaaag
    29521 agaatcaaaa tatagaagtt aaagaagaaa aaataaacaa aaaagagtgt aatcatgatt
    29581 atgatgtagt ggtaatagga ggaggacctg ggggatattt atcagcatta aaagcagctc
    29641 ttttaggtgg aagagttgct ttagttgaag aaaatatact aggaggtact tgcttaaata
    29701 gaggttgtat ccctacaaag acttatataa aaacagcaga aatattagaa gaaattgacc
    29761 aattatcaaa gagaggtgta aaagtaactg tagataaaga acaggacatt aagaaggcca
    29821 taaaatataa aaatagagta gttaaaaaac ttacagctgg tgttggcgga ttattaaaaa
    29881 gtagagatgt agatgtgttt aatcttaaag ctagtgttaa agaagagcat aaagtaattt
    29941 tatcagatgg aaaagtttta gatacagaaa atataataat tgctacaggt tccaaagtaa
    30001 ggatattacc tataaaagga atagaatcaa atttaataat aactagtaca gaagcattag
    30061 atttggaaac agtgccagaa gaattagtaa taattggtgg tggagtgatt ggttgtgaat
    30121 ttgcagagat attcaattca agaggttcta aagtaactat agtagaaatg gaagatagag
    30181 taatcccaag aatggataaa gagttaagcg aatctttaaa atattctctt agtaaaaaag
    30241 gtataaatgt attaactaaa aagaaagttt ctgaatttaa agaagaagga aataacatat
    30301 tagtgtgcat tgaaggtgaa gagccaataa aagcagattt atgtctatat gctataggaa
    30361 gagaagctaa tctttctggc atagaggact tagatattaa aatagataaa ggttcaatag
    30421 ttgtaaatag taaaatggaa acaagtatgc caagcatata tgcagtaggt gatgttactg
    30481 gaggtgtaat gttagctcat gctgctttta aaatgggaga ggtagcagct tcaaatgctc
    30541 taggtgtgaa taaagaagtt gacttaggtg cattacctag ttgtgtttat acaattccag
    30601 aagtagcatc agttggtatt acagaagagg atgctagaaa aaaatataat gtaaaagttg
    30661 gtaagtttaa ttttgctgga aatggaagag ctttagcttc tggtcaagaa caaggatatg
    30721 tgaaggtagt agcagatgct aaatatggag aaattttagg tatacatatg tttggatgtg
    30781 gtgtagcaga acttataaat catgcagcat catttaaagc gttagagata cctactgatg
    30841 aagcaagtga gttgatattt ggtcatccat gtacttcaga agctttaatg gaagctttgg
    30901 cagatgtaaa tggagaatgt ttacacttac ctaaaaaata aatctaaaca taaattatta
    30961 atttaagtct attattaata ttaatttata tcataaaatt ttaaaacact tacttagcca
    31021 taccccttat tatgcaaata atttatttgc ataataaggg gtatttttta tggaaaaata
    31081 tagaatatag aatccttgta gtttttgttg tacaaaaaag atagtaatat aaaataacat
    31141 tatcatttta gaatgatttc aattgtaatt aggaaaagtg atttttttgt ttttaaaaaa
    31201 agtattattt gtaaaatata aaaaaaaatt tcttaatatt tgaataaaag aatagcttaa
    31261 tttaaatgtt attactttat aaaaaaataa taagtacaaa aaaaacaata tatggcatat
    31321 aagttgctat ataaataata aggagggaac aattataaat gaggcaaaga ataaaaaact
    31381 taattgaaaa tgaagataag aaaaatcctt taacagatga agtgatttca agtaggttaa
    31441 atattcgaag agaagatgtt acctttttga gaaatgagtt aaaaatagaa gattcaagac
    31501 aaagaagaaa agttgtttta attaaagcta taaaagatat tttaaaagaa gaaaaaacta
    31561 taaataaaaa tgatataaca agaagattaa acagcattgg gtttacagta tcaagattta
    31621 cagtcataca atacttaaaa gagattcaag attctggtga cctaaaactt gataaagaga
    31681 gtgatgataa aaaagaagaa ataatcaata atagagaaaa taaagatata gcatttgata
    31741 aactcatagg tagtagtggt agcttaagta caattataaa actagccaaa gcagcaattc
    31801 tttatccacc tcatggtctt catactatta ttatggggcc aactggtgtt ggaaagagtg
    31861 agttagcaga gtgtatgtat aaatttgcag tagaatcatc cagattccct aagaatgcac
    31921 catttatagt ttttaatgca gcagattatt cagagaatcc acagcttttg ctatcacagt
    31981 tatttggaca cgttaaaggt gcttttacag gagctgatga gaacaaagaa ggattagtat
    32041 caaaggcaga tggaggaata ttgtttatag atgaaataca tagattacct catgaaggtc
    32101 aagaaatact atttcaactg attgataaag gtatggtcag aaagttagga gaaacaagac
    32161 taacacataa aatagatgta atgattattt cagcaacttc tgagccaata gattctcatt
    32221 tacttaatac atttaaaaga cgtatacctg taacaattga aataccagaa ttagttgcaa
    32281 gaccattaaa tgagaggttt gacattataa ataatttctt cttaatagaa gctcaaagaa
    32341 tgggagcaaa catacatata aagtcagatg tattaaaagc tcttatgctt tacgattgta
    32401 taggtaatgt aggtcaactt agaagtgata ttcaagtagc ttgtgctagg ggactattaa
    32461 accaacttac aaacaaacta aaagaagtaa atataagcat atcagatgta cctggatatg
    32521 taaaacaagg tcttataaaa atcagaaatt gtagaggaaa aatagaaaat tatgtagatg
    32581 gggatttaat tatagactca aatcttcaag gtgagataga aaagaaaaat gaagatatat
    32641 atacttttcc agatgaaata tacaaactta ctgaaagaag acatattgat ttattggaac
    32701 aaggtttaga ttttgatgtt ataaatagaa tcattggagg agaattagaa agttgcattc
    32761 aaaaatacat aaaacaagtt aaaaaagttt ctattagtaa caatcttcca gatataagtc
    32821 caattgttgg aaaatatatc gtagagctaa cttatgaagt aatcaaaata gcatcacaat
    32881 acctaagttc actagaaccg aatctacatt tatgtttagc agttcaccta aaagcagctt
    32941 ttgagagatt aaaagaagga aaagtaatat caaatgctca tttgaaagaa ataataggaa
    33001 attatccagt agaatattct gtagcagaaa aggtagctca atatttaaag gaaagatata
    33061 acataaactt accaaaagaa gaaataggta taatagcaat atattttaaa atgagtagta
    33121 gaagagatat gtctgaacaa aaaaagatag gtgtacttct tatggctcac ggaagagtag
    33181 catcttctgt atgtgaggtt acaaataaac tattaggaat taatcacgca aggtatttag
    33241 atatgccatt tgagaagaaa ccggaagaaa tgattgaaga agctactaca ataataaaag
    33301 atattgatga aggtagaggt gtgattatat tagtagatat gggttcactt tcatattttg
    33361 gagatattat ttctggaaag actggaatta atataaggac aatatctaga tttgatacat
    33421 tgctttctat agaggttata cgaagagcag ttttaccaga tttaacaata gacgagatag
    33481 ttgatgaagt taaaggtata gagccacaag taaaagaaaa ggttagtaaa tgtaataata
    33541 caaaagtaga ttttagaaaa cctataattt taacattatg tattactgga ttagggggag
    33601 cttgtaagat aaaaaaaatc ctagaggaaa atattaaaag tataactgaa aaggttgata
    33661 tagaacctat aggtatgatt gaatatccaa gtcttgattt agagataaaa caaataagca
    33721 atgataaaaa tgtaatcgcc atagttggaa caattgaccc tcagatagca aatatccctt
    33781 tttttagtct agtagacata aatgataaag caaagttaaa tcaaataaat aacctaattg
    33841 atacaaatca aattggtgaa acaaataaaa aatatgaatt atcagattta atacataaag
    33901 aagatatatt tgtggactta caggttaaat caaaagaaga agttctaaaa tatatgagta
    33961 aaaaacttag agagaaaggt tatgtgacaa aaaatttcta taaaaaagta gtggaaagag
    34021 ataacttatt tccaactgaa ttatcagatg aaatagcaat acctcacaca gatggaatag
    34081 atgtaattag gccagctata agtatagcca tcttaaagaa gcctattgtg tggagtaaaa
    34141 gtaaagtaaa aataatactt ttattagctg tagaccaaaa gtgttttaat ccattaagca
    34201 ctctactctc atttatagaa acagatgaat ttaagaagat aaaaaacatt aaagatagag
    34261 attttattag ggagatgata ttagatggag tcaatgaaaa taattagtag caatttaatt
    34321 tttaaaaata ttgaagtgtc taacaatgaa gatgcattaa agtttttggg acaacgtttg
    34381 tttgacgaac agtatgtaaa agaaagctac atacaagctg ttgtagcaag agaaaaaaag
    34441 tatgctacag ggttaccaac agaaatatac ggagtggcaa taccacatac agatatagta
    34501 catgtaaatg agccaggaat agcaattgga atactgaata aacctgtaaa atttataatg
    34561 atgggtacag acgatactga aattgatgta aaagttgtat ttatgttagc agtgaaagag
    34621 ccacaagaac aattgcaact attagaaaaa cttatgacta tatttcaaga taagaatata
    34681 ctaaataata ttgttgactt aagtgaagaa agtgtgagtg atttattaaa ctctaaattg
    34741 aaaaattaaa catgtaaagt atattaaaaa tgtaaaaaga ttattaaaga tttctaacta
    34801 tagatatctt taaaataaaa taatttcaat gggggtgact aatgtggcta aaaagatatt
    34861 agttgcatgt ggaacaggag tatgtacttc aacaattgct ataaacaaat taaagaaagc
    34921 acttcaagac attggtaaat tggatatggt taatattaca caatgtaaag tagtagaagt
    34981 tgcatcaaaa gcacctgatt atgatttaat aatttgtact acacaagtat ctagtagtat
    35041 aaaaacacca gtgataaatg gattaccttt tttaactggt gtcggaatgg ataaattaat
    35101 caatgatgta ttagaagagt tagaattata attaaaaaaa ttaaggaaca tggtctctaa
    35161 aattataagt tttagagact taaaattaaa taatggaggg tctttatatg gagatattaa
    35221 atttcattgt tggtctaggt gcacaagtaa tgatgccaat aataatatgt atttttggtt
    35281 tgatattagg tacaaaacta ggaaaatctt taagagctgg tttaactgtg ggagttgggt
    35341 ttataggtct taacactatt atagctttgc taacagataa tttaggtcca gctacacaac
    35401 aaatggttaa gaatttaggg ctaagtttat caataataga tgtaggatgg ccagcagcat
    35461 cagctatagc ttttgcatca acagtagggg ctttaattat tccaataggt cttgtggtaa
    35521 atatagttat gctaattact aatactactc aaactgttga tgtagacata tgggattact
    35581 ggcattttgc ttttacagga gctttagtgg caggggctac tcaaagtgta atgtggggag
    35641 tatttgcagc agtagcaaat atggtaattg tattggtaat ggcagattta actgctccag
    35701 gtatagagga atatttaggt atgccaggta tatctcttcc tcatggattt acacaggcat
    35761 ttgttccaat tgctatagta gtaaataaat tattagacct aataccaggc ataaataaga
    35821 ttgagataaa tgctgataca ttgcagaaaa aatttggatt atttggagaa cctttaataa
    35881 tggggtctgt aataggtgta ataattggta ttgctgcgaa atatgatata aaaggaatat
    35941 tacaattagg tgtaactatg ggagctgtat taatattaat tccaaagatg gcagcattat
    36001 taatggaagg attattacca gtatcagaag ctgcacagga atttattgag aagagattta
    36061 aaaacagagg caagatttat ataggcttag attcagcagt aggaataggt catcctgtta
    36121 cattatcagt tgcactagtg ttggttcctt tgactatact tatagcagct atattgccag
    36181 gaaataaagt tttaccattt gcagatttag cagtaattcc atttgcactt gtattaatcg
    36241 taccaattac aaagggaaat gtgtttagaa cattaataat aggaataatt ataattactt
    36301 ctggattatt aatcgcaact aacttagctc cattatttac ccaaatggct ttaaatgcta
    36361 gttttaaaat gcctgaagga gcaactatga tttcaagtat ttgtgatggt gcgaatccat
    36421 taagttgggt atttgtaaaa gttatgaact ataaagttat tggaggagta gtttttggag
    36481 ttatagcttt aggaatggct atttacaata ggaatagaat aataaatgaa aataaaaaac
    36541 tatctataga agaataaggt taaaacaatt ttattaagga gtgtataggt tatgaaaaaa
    36601 tataaatatg attctaaaag gtacttactc agtgttacaa taccaggatt ttttatgatt
    36661 ttaatattga tttatgcact gtttaataac tatacaaatt ttaatttgaa tatatatagt
    36721 ttgttaataa ttgtctgtac ttatggtata ttcaatactt tcatatcatc aagtaatcct
    36781 aaaaaaatta ttattgacag caaatgtata cattttacct catttatgac tacacataag
    36841 tatgaaatta aagatataga aaagataaga ataagagaat tttataataa aaaaatttat
    36901 ctaaaaatta atgatggaaa acttttcaaa ggtaggtatt ggataagaac aaatatgttt
    36961 aataatagta tagcattata tagctatttc attgagttgg aagagtgctt atatccagat
    37021 tcactaaaat ttagaaataa aaaatttgag aataagaaca tataaaaata aaaaattgaa
    37081 ttgaagcctg gcttaatctt ttttgataag atataagttt gaactttata ctaaattttt
    37141 agaattttca atcaagacaa taacagaata gctaagcaat tagaacacaa ttaagatgta
    37201 taaggcatgt atatgatgaa tgggaggatt taattaatgt tattagagaa attaagaaaa
    37261 gaagtattac aagcatcttt agacctttta aactataatt tagtgacatt aacaggagga
    37321 aatgtaagtg ggagagatga acaaactggc tatatagcaa tcaccccaag tggaatggat
    37381 tatagaaatc ttacagcaag tgatattgtt atagtagatg tagatggaaa tattatagat
    37441 ggaaaatgga aagcatcagt ggatttatca gaccacttgt atatatataa gcatagagaa
    37501 gacataaata gtataattca tacacattct acatactcaa gttgttttgc tattttaaat
    37561 gaacctatag aatgcgcaag tacaactctt gcaaatgaag tagggggttc agtaccagtt
    37621 gcaaaatttt caccaccaac atctaaaaag atgggaaaat gtgttattga agcaattggt
    37681 gacaaaagag catgtctttt agcaaatcat ggcgtaattg cagttggtcc atctgtagga
    37741 catgctctta cagccgctgt aatgttagaa gattcagcaa aagtatatta cttagcaaaa
    37801 tcaattggca caccagtgct actgcctgat gaagaaattc aaagagcgag agatgtattt
    37861 tttaatgtct atggtcagga taaataaatt agataaaatt ttaaaagtaa tatgaaaata
    37921 tagatattca atgatattaa ggagatttat aatgatataa taagtttaat agttaagaag
    37981 taaaatatga aatgtaatag tatataaatt catttaagac taaaaagaat aagataaaat
    38041 aaaaaggagt ctgtatttct atgtattact tattaaacaa aagcacaaat ccttacttta
    38101 atttggcatt agaagaatat ctatttttaa atgataaata taatgatgat attataatca
    38161 tatggagaaa tgaagaatcc atttttatag gtaaaaatca aaatccatat caggaagtat
    38221 atcatgatgt tattgaaaag ggagaaattc ctattttaag aagaatatct ggaggaggaa
    38281 ctgtttatca tgacttaggt aatatcaata tgtcattcat acaaaaagat aggcaacttc
    38341 atgagataga ttttttggag catactaagt ttatgcaaaa gatgctttca accttaggac
    38401 tagatgtatc tataactgaa agaaaagatt tatttttaaa tgggaaaaaa atttcaggtt
    38461 ctgcacagtc tataaaaaga aaaaattctc tttatcatgg aactcttctt tacgattcag
    38521 atttaaataa attaactaaa tatttaaatt caaataaagc aactgaatca aatgcaacta
    38581 aatctgtttc aagtaaagtt acaaatatta agtttttact cgaaaaagat ataaattact
    38641 ttttagatta ttgtgttgaa tatttaaaat ctaatataaa gaatataaaa gaacttgaat
    38701 tggatgaaga agatattaaa agtgtataca atataggaga agataaatat aaaaagatag
    38761 agtggacata tggtaaaacg ccgaaatttc aagtagtgct accatttaat aatgaaagta
    38821 aaataaatat acatgttaac agatggaaag tatcaaaatt ttttatttct tatgatgata
    38881 atgtaattaa tatggacaag ttgttagatt tgaatttctt taaagaagaa ataaaagaga
    38941 gtattttaga aaattatcca cattacatag atttaatcaa tttaatattt taatataaaa
    39001 cctctaattt gataaaattg tcttatatca aactagaggt ttttaatatt cttgcatatt
    39061 aacaaccatt acaaaattgt aaaattaa
//
LOCUS       DAEMGG010000025        38654 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEMGG010000025 DAEMGG010000000
VERSION     DAEMGG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38654
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.025"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..896)
                     /locus_tag="KPK24_001252"
     CDS             complement(<1..896)
                     /locus_tag="KPK24_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG2298939.1"
                     /translation="MKLKHIAIIGSLFPILFSIVLFFGVLMSADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAKEYGISEYVNVLLAIIQVESGGTAKDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQMQYDATQHIPLSQA
                     KAGELVFFHSTYNAGSYVTHVG"
     gene            complement(893..3085)
                     /locus_tag="KPK24_001253"
     CDS             complement(893..3085)
                     /locus_tag="KPK24_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2298940.1"
                     /translation="MKERIQSAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPENEDGKTREEVVKTEIESNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYMAHRARRMERRLAGAVTAGGVAGAVAGSTVSNARTTRNNSAKDNRGNTSSSMGQR
                     AGSKVGAVLDTKNKVKDKANAMKETVKDIPTQTAYAVHSAKEKAKTSVSDFKRGMVQE
                     QQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQARKTDGSATTGATRPHERPVTASA
                     IPKTNVEKTQEVKRPATTSTSKAGEPVKTNSVKERPLSSGTSDGKIPQSAQTVHSKNV
                     EKVVSHETHQNYKAERTTKAQTVEQAKRTTEHTEKNRNLVTKKGQKKK"
     gene            complement(3085..5535)
                     /locus_tag="KPK24_001254"
     CDS             complement(3085..5535)
                     /locus_tag="KPK24_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2298941.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNLKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEHQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRCEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGEILSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPIQRNEVE"
     gene            complement(5522..5911)
                     /locus_tag="KPK24_001255"
     CDS             complement(5522..5911)
                     /locus_tag="KPK24_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2298942.1"
                     /translation="MKKIRSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPAFLTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLQKEIFNETITAVRSVSYVPD"
     gene            complement(6029..6532)
                     /locus_tag="KPK24_001256"
     CDS             complement(6029..6532)
                     /locus_tag="KPK24_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2298943.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIEIGEYVSIDELNEMYEMIEELPDYIVDCLDEFIGHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            complement(6535..7038)
                     /locus_tag="KPK24_001257"
     CDS             complement(6535..7038)
                     /locus_tag="KPK24_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2298944.1"
                     /translation="MQETRVLVETKRTTGEETTSYWFDLPIEVAEFEEKLGVSTESQD
                     YRIIEKLLPYADEVHEHTSVYQLNELDYMYRQLSSDMQEEYTMLHTVYENLEALYICR
                     NVITLYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFETYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(6957..7253)
                     /locus_tag="KPK24_001258"
     CDS             complement(6957..7253)
                     /locus_tag="KPK24_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2298945.1"
                     /translation="MNDIFRDMQAKVGCENISDLPSYKRKVWHEMKQLNLADYEEKQL
                     EDFSKYVFGMSCQTLKDVMKQQKGREEQCRKQGYWWKRKEQLAKKQHHTGSTCR"
     gene            complement(7350..7952)
                     /locus_tag="KPK24_001259"
     CDS             complement(7350..7952)
                     /locus_tag="KPK24_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2298946.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            complement(8037..8198)
                     /locus_tag="KPK24_001260"
     CDS             complement(8037..8198)
                     /locus_tag="KPK24_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298947.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALVAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
     gene            complement(8365..10275)
                     /gene="ltrA"
                     /locus_tag="KPK24_001261"
     CDS             complement(8365..10275)
                     /gene="ltrA"
                     /locus_tag="KPK24_001261"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG2298948.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNHILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11017..11151)
                     /locus_tag="KPK24_001262"
     CDS             complement(11017..11151)
                     /locus_tag="KPK24_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG2298949.1"
                     /translation="MWELLKDLLLVSFGMGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            complement(11159..12358)
                     /locus_tag="KPK24_001263"
     CDS             complement(11159..12358)
                     /locus_tag="KPK24_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2298950.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPNNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIGYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIRKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAELTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTQPEPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHYQIIKQQTATVEE
                     MVANVKE"
     gene            complement(12560..13954)
                     /locus_tag="KPK24_001264"
     CDS             complement(12560..13954)
                     /locus_tag="KPK24_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2298951.1"
                     /translation="MKMILNKGHRIRASDKNLVYHFSIGTLLFVFIAVILLLNMKQLM
                     RTDWEHFSLLDNGLTLSTYNFITLLIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIIWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTMLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFSSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            complement(14071..14556)
                     /locus_tag="KPK24_001265"
     CDS             complement(14071..14556)
                     /locus_tag="KPK24_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298952.1"
                     /translation="MAKEKGSFKEFLSAIAPEHQAFVEKLNTKLIEQGCDLVIKEAKS
                     GYTASYQLEKKTVMNWVFRKTGVFARIYGDNAGKYEDIIASLPADMQKKMTTSRDCKR
                     LIDPNACSDTCVKGFVYALNGDTHKKCRNDGMLFVLTNETAEHIAGLVCAEVNVRKSA
                     L"
     gene            complement(14654..15037)
                     /locus_tag="KPK24_001266"
     CDS             complement(14654..15037)
                     /locus_tag="KPK24_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2298953.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRAENDDGSTSEEITRRT
                     YDLKCSVQERMIQVSIPADIPVKDYPYNAEVELVNPVADTVANASYRGVNVDWYIKAD
                     DIILKNKGGQVGNPQNHAPQGQPKK"
     gene            complement(15052..15378)
                     /locus_tag="KPK24_001267"
     CDS             complement(15052..15378)
                     /locus_tag="KPK24_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2298954.1"
                     /translation="MELKYVVPNMAQTFGNLEFAGENKIEQQRINGRFTPVSRSYNLY
                     SDVQRADDIIVTIPAKAGEKHFKPEQRVKLINPKITADGKAVKGRGFTNYILTADDML
                     PAEESK"
     gene            complement(15614..18658)
                     /locus_tag="KPK24_001268"
     CDS             complement(15614..18658)
                     /locus_tag="KPK24_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG2298955.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPTTLVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTDNHNGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITNGNG
                     NYSIAGATYGVFSDKDCTKQIATLTTDDSGNTEVAEVKATTVYIKELSAPLGYKVDTT
                     VYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTTAETDSNGTTHYITKLADAYKVSGDSFYMQDGKAFLPL
                     GTLTVEETKSPTGYLLDGAYMQANGSEEQIKALYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETKDTKPQGSATLKDTAFDIISLNDNAVLVEGKIYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPNGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSSKWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGMELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEITIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAEIIIDGKRVESDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPMKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTKYKLVGWQMLKEENAELLINGKRVESDYTFIADSETMKVEVTFTFDATSL
                     DGKQLVTFEELYDLSNPNEPKKVTEHKDIEDEGQTITFKEKPEVPEKPETPTTPEKPS
                     RPTDSPKTGDNTNVMAFLVMLLTSAGGLAGTYLYKRRKMKKS"
     gene            19186..20193
                     /gene="asrA"
                     /locus_tag="KPK24_001269"
     CDS             19186..20193
                     /gene="asrA"
                     /locus_tag="KPK24_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG2298956.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKDVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            20194..20985
                     /gene="asrB"
                     /locus_tag="KPK24_001270"
     CDS             20194..20985
                     /gene="asrB"
                     /locus_tag="KPK24_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG2298957.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            20998..21969
                     /gene="asrC"
                     /locus_tag="KPK24_001271"
     CDS             20998..21969
                     /gene="asrC"
                     /locus_tag="KPK24_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG2298958.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            22026..22802
                     /locus_tag="KPK24_001272"
     CDS             22026..22802
                     /locus_tag="KPK24_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG2298959.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            23073..25091
                     /locus_tag="KPK24_001273"
     CDS             23073..25091
                     /locus_tag="KPK24_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG2298960.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            25306..26163
                     /locus_tag="KPK24_001274"
     CDS             25306..26163
                     /locus_tag="KPK24_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2298961.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            26260..27129
                     /locus_tag="KPK24_001275"
     CDS             26260..27129
                     /locus_tag="KPK24_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2298962.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(27500..28132)
                     /locus_tag="KPK24_001276"
     CDS             complement(27500..28132)
                     /locus_tag="KPK24_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG2298963.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(28204..28557)
                     /locus_tag="KPK24_001277"
     CDS             complement(28204..28557)
                     /locus_tag="KPK24_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG2298964.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(28635..28838)
                     /locus_tag="KPK24_001278"
     CDS             complement(28635..28838)
                     /locus_tag="KPK24_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2298965.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            complement(29228..29458)
                     /locus_tag="KPK24_001279"
     CDS             complement(29228..29458)
                     /locus_tag="KPK24_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2298966.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(29460..29867)
                     /locus_tag="KPK24_001280"
     CDS             complement(29460..29867)
                     /locus_tag="KPK24_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2298967.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            complement(30227..30676)
                     /locus_tag="KPK24_001281"
     CDS             complement(30227..30676)
                     /locus_tag="KPK24_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2298968.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            complement(30690..31616)
                     /locus_tag="KPK24_001282"
     CDS             complement(30690..31616)
                     /locus_tag="KPK24_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298969.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(31741..32145)
                     /locus_tag="KPK24_001283"
                     /pseudo
     CDS             complement(31741..32145)
                     /locus_tag="KPK24_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            complement(32295..32915)
                     /locus_tag="KPK24_001284"
     CDS             complement(32295..32915)
                     /locus_tag="KPK24_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2298970.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(33014..34282)
                     /locus_tag="KPK24_001285"
                     /pseudo
     CDS             complement(33014..34282)
                     /locus_tag="KPK24_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            34374..35189
                     /locus_tag="KPK24_001286"
     CDS             34374..35189
                     /locus_tag="KPK24_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBG2298971.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSSRAEMERL"
     gene            35861..36628
                     /locus_tag="KPK24_001287"
     CDS             35861..36628
                     /locus_tag="KPK24_001287"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG2298972.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(36956..37633)
                     /locus_tag="KPK24_001288"
     CDS             complement(36956..37633)
                     /locus_tag="KPK24_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2298973.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(37783..38100)
                     /locus_tag="KPK24_001289"
     CDS             complement(37783..38100)
                     /locus_tag="KPK24_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298974.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(38122..38460)
                     /locus_tag="KPK24_001290"
     CDS             complement(38122..38460)
                     /locus_tag="KPK24_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2298975.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT        12050 a         6817 c         6649 g        13138 t
ORIGIN      
        1 attcccacat gggtaacata cgaaccagcg ttataggttg aatggaaaaa gaccaactcc
       61 ccagcttttg cctgcgataa agggatatgc tgggttgcgt catattgcat ttgtgccgtt
      121 ctcggtaaag agataccagc ttttccataa caccattgcg tcagtcccga acagtcaaag
      181 gaagtgttgg gattgctacc gccatacaca tatttccagc cttgatattt caacgcttca
      241 ttcattacct tttgtgccag ttctcccgat acctgcggaa cagttaaata ctgattgact
      301 aattccacat aaaacatatt tccatagcca taccgccagc ccccattttt cgcaacggct
      361 atcgggttgg tgtaggttac ttttttaccg ccagatttct cacgggcaaa gctctctgca
      421 aggttaaaag tatgtttctt tccttttcct gccacatatc ccacatagcc accgccatag
      481 ttataggact gtatcgccac attcaaatca tttattcctt gatttttgca ggaagaaagc
      541 agggacgcaa aatatttaca cccctgcttg attgagcttt ctgtatctaa cgagttgggc
      601 ggtaaaccaa gactttctga actctgcatg acatcttttg ccgtaccgcc actttctact
      661 tgaatgatag ccagtaacac attcacatac tcagaaatac cgtattcttt agcgtacttt
      721 tccaccatag gctgatgttt caagacttct gcggataaat tcatacccgt aatgcccgaa
      781 gaaaagttgc tgttctcgtc gtcgctgtcc gcactcatca gtaccccaaa gaaaaggact
      841 atggaaaaga ggataggaaa cagactgcca atgatagcga tatgtttcaa tttcattttt
      901 tcttctgtcc tttcttcgtt acaaggttac ggttcttttc agtatgttct gttgtacgct
      961 ttgcttgttc aactgtttgt gcctttgtgg tacgttctgc tttgtaattc tggtgtgttt
     1021 catgcgatac cactttttct acatttttac tatgtactgt ctgtgccgat tgtggaattt
     1081 ttccgtcaga tgtacccgaa gataagggac gttctttcac actatttgtt ttgactggct
     1141 cgcctgcttt ggaagttgaa gttgtagcag gacgtttgac ttcctgtgtt ttctcaacat
     1201 ttgtttttgg aatggctgaa gctgtgactg gtcgctcatg gggacgggta gctcccgttg
     1261 tcgctgatcc gtccgtcttt cgtgcctgcc ttgcttcttg tgctttttga agttccatac
     1321 gcttgtcagc gatattcttc ttatgctgtt cctgcttttc caagcgtccc gactgtctgg
     1381 attgttgttc ctgcaccatg ccacgcttga agtccgatac acttgttttt gctttttcct
     1441 ttgcggaatg gacggcataa gcggtctgtg tcggtatatc cttgaccgtt tctttcatag
     1501 cgtttgcctt gtctttgacc ttattcttcg tatctaagac agcacccacc tttgaaccag
     1561 cacgttgtcc catgctggaa gatgtattcc ctcggttgtc ttttgctgaa ttattccttg
     1621 ttgtccttgc attagaaacc gtactgccag ccaccgcacc agcaacaccg ccagcggtaa
     1681 cagcaccagc aagccgtctt tccatacgcc tagccctatg tgccatataa agatacggtc
     1741 tgcggaaaat tcttcgtccc atgctttgac tgtcgttagc gttcaaagag aacatactca
     1801 ttaaatcgcc taacttcatg tagataccag cgaaacacac tatctgcaag aacgccacca
     1861 taaagaacgg gtagtctgtg gaaatgttat aaaacatact ggaaatgcta aatgccaccg
     1921 ttacaatgag cgtaattcct gcccttgtca ttatggtatt aaataccctc acgattgcct
     1981 gctttgccat gctttcatag ctcggtatca tggatagtaa aaagctgata ggtaaaaaca
     2041 tggcgaagat aataaaaagt atctggctga acaacatcat acccgtaagc aagaatacaa
     2101 atatcgtgat acctaagttg aagaacaaga ggaagaacac catacctaaa cggtttacga
     2161 cttgcggtat cgtcagattg ttgttgtcgt tactttcgat ttctgttttc acgacttcct
     2221 ctctggtttt gccgtcctcg ttctctgggc ttgccgatac aagagcttct acacggtctg
     2281 tcccgatttc ctctgcgttg ctgttcccaa actgtaagag tagccacggc tgttccactt
     2341 gaatagagaa taggctgtcc cttatcaagt ccacgctgtc cttgccctcg ctgtcagagt
     2401 ttgggagcat gatttttgtt cccaagtcca aagaagcggt actgatgtcg cttgaaaatt
     2461 catttatctt tttgatgtag tcgggagcat aggcaataaa tgaagccgac aaaataaaca
     2521 ccacaacaaa gttgataacg gcgtgaagtg ccttgctggt ttctctttta atcagtcccg
     2581 tataggcaac atatagtccc accactaaga taatgagaag caggaaaccg acatagaagc
     2641 cagaggttga aaaaccgctt gaggtaacgc ctgcaagggt ctgtatgctc tgcccgatac
     2701 tgtctgccat atcattgatg aagtccagct tgtaggcttc ctgcactaca tagcccgtgg
     2761 cattacttaa atataaactg atagtccaga caaaattagt gatacagtac aaaccatatt
     2821 gtactgattt tccaatcccg tccagccagt tccacggcaa ccaagcccag ctattatcca
     2881 catagaagtc aagctgatag ttggaaagcg gatatttgga atatagattt tctgcgttta
     2941 tcgtatcgtc cacaagcccc gtcgcatgag ccaccgtccc cagaagtgaa agcaggataa
     3001 gggagagtgc caccacgaac agagccattt tgagaaagtg gaaaatcttc ttttttgtga
     3061 acgcactctg tatcctttct ttcatcactc cacctcattt ctctgtatag gcggtctggt
     3121 atcaaaggcg tgtagcagtt cttcaaagac ggggtgtatc tgtaccacgc cgacacgccc
     3181 gtataagtcc tgcaacaagc attgtccgtt ctccaaatca cgaagtcgct tctgattgtt
     3241 ttcgtcctct ttgtcgatac cgaaaaattc taaagtctgt tttatctcgt tgatgtcagt
     3301 agaacggaaa gcgaatttta agccgatatt gtttttcaga ctttcctttg acacgtcccc
     3361 agaagattgc gtaacgaaat acacacctgc ctgcatagct cgtccagcac gcaccagctt
     3421 gttagataaa atttcgcctt gtgccacatt taagaacgcc cacgcttcat caaggtctac
     3481 gattttgaag atagagcggt cgctgtggat aaaatctaag gcaaaggtac taatcacaat
     3541 cagcatggaa accgatagta attcaatggt cgtgtattcc tcaaaagtcg tgtccttatc
     3601 tggcagtaca aggtcggcta cttgaatgat attgagctga ttatctaaac tgatagcatt
     3661 ttccaccgtt ccgtctgaaa acagaagatg tgcaaaatca tagtctgtaa agctgtcgat
     3721 atggtctgcg atattgcgtg aaattgcggt atcttcacag cgtaactcgt ctatcacatg
     3781 aagcaagccc cttgtttcac tctgggtaac agaacgcacc gccttacgaa gtacggggaa
     3841 tttttcgccg tccctagagg aaataccagt taaaaaggtt agtatgtcga ttgccagact
     3901 ttcagcgtct ttcacattct tcataatcac aaacgggtca agaagccctg cgttgtcctt
     3961 gtcgcttgtt aggtttacga tattgatttc atgggcgatt tctggaagtg tttctttcca
     4021 gtttccacgc tctgattttg ggtctaagat aaccgcttga ccgccaaaca aaacagaata
     4081 gtacacaagc aggttattac agaatgactt cccaccgcca agcgaaccaa caaaagcaga
     4141 agcaagggca tttgtaactg taccttttac gccttgactg gcaagggacg gttgcagata
     4201 tacatttctt cccgtatcaa cggaatagcc catatagata cccgtgtttt cccctaactg
     4261 ctgtgtcgca ccaaaaccaa gcccagctaa gaagtctgat tttacatact gcacataatc
     4321 attgatatat cgcttgctgg ctggtagaaa ttcagaatga agccccagca tatccccagc
     4381 aggacgtact aacttcacat tcaaatcgtc ataaaaatct ttgacttcat cacaacggcg
     4441 tttcagttcg tccaaatcat cagcagacac acggataaca taagagagct tatacatact
     4501 ttcttttgtc tggtctaaat cggtttccag ctcgtccacg ctgtctaaag cgtctactac
     4561 atttgagctg gtttcacttc cagcttgata agcgtgatta tcaaggtctt tcagttcctt
     4621 tttcttgttg cggacggtcg ttaatgcttt tcggttttcc acgatttcta cattcatgct
     4681 cgtatcaacg ggaaaggtaa attgctgttg ctggaaatag aagatttcac tagacggaaa
     4741 atcaagctcg cctacaatcg cattgacggt aaaataggac acataacttt ccttgtcctc
     4801 atgttccaat cgtaaatatc gctggctttc ctcaatcaca caccttgtcg ggcggataag
     4861 gtcgtaatac ttgataagtg tttctttctt atactttttc tttggtagct ggtgctcata
     4921 atcttcatag gcgataccgt ccctgccgta aagatgttcc ataagatacc caaagtcatt
     4981 gatttccaga cggcgaacct taaagcgacg ggagatttta ttttccagta acttttccat
     5041 tttcatgtaa cggttgattt catcattcgg catggaaaca aagtcgttca tcagcgtgtg
     5101 gttcacttca tggagaaact ctttgaacgt cagccacgcc gattttttaa ggttcttcag
     5161 attgagctgt tcttccgtaa ccatgagttt aaagccaaga aaaaagcggt agtccacttg
     5221 attgtcccca atcattgata ctaacgcttc ggtctgttcg tctatcttct ggtaagcgac
     5281 ttcctttaat ttccccgtaa ccaatctttt tgactgctcc tgcatacttc ttatagagct
     5341 ttccgtggca atctgcaaag catgaatttt accctcacgg gactgtgcga ttagctgtcg
     5401 gaaactgtca tgcacgataa atttctgttc tgcggataag aaagaatagt tatacggtat
     5461 cagctcatag taggcgaaca cctcattgtc cttgttccag acaaggttgt tgtcaatata
     5521 tttaatcggg aacataactc acactcctta ctgccgtgat tgtttcgttg aatatttctt
     5581 tttgcagttt tacggctttt cctgcataag tgattttcgg tcgcaacgca taggttatct
     5641 gtgatttcaa aaagctgtat ggcttctttc cgtcaaaggt tttctgcgac ataaaccaag
     5701 tgaggaaagc aggaataccg aagtatttta aaaatgctcc ctcaatcatg gaaagtgggg
     5761 gcatatcccc aaacagaatg atgataaatt ctgtaatcac aaaccatgta atctgtgtaa
     5821 aggtaacggg aaagggcaag ttaaagtcat tgattgcata caagactttt tctacattcc
     5881 agatacccgt gtagcttcta atctttttca agttctttca actcctctca taagtttcta
     5941 aatggaaaag gacagccctg tttcagactg tcccttaaag agaaatacgc cgtcagtagc
     6001 aacaatgctt gtcgctgatc ttccagcgtt aatatggtat ctcgcacatt cctcggcttg
     6061 ttgcaatgaa gcaaccgccc atatccaaat ctcgcccgta tgcttcatag tcaatatagt
     6121 tctgcaagct agggggaata tcgccaagtg cctgcaattc atctatgtag taataggcaa
     6181 catctgtcat ggtttcacag tcgggataat agtaaatatc gtccttgtgt tccacgactt
     6241 cctccagcgt cccataatga ccaataaatt cgtccaaaca atccacgata tagtcgggaa
     6301 gttcctctat catttcatac atttcattga gttcatcaat ggaaacatac tccccaatct
     6361 caatcgggaa gttatcggta tcatggattg cgtattcttc atactgttca ttcaagccga
     6421 ttttttcttt cacatcttcc tcgtcaatag ggaaagtaaa ccaagcaccg actaaatagc
     6481 cctcattata cttgccaaga ttagcgatat aaactgccat atcatcaatc atacttactc
     6541 tggcaattca aagataccgt gttccgttac taaaaatttc ccgtgttcct gcaagtgtga
     6601 agcataggtt tcaaagtcaa aatagtattc ttgacagtcc tcggataaat gtttgaatgt
     6661 agggtcattc atcagctttt gccttgcaac atcaatcatg cttttacagt cgggataaag
     6721 cgtaattaca ttcctgcaaa tataaagtgc ttctaaattt tcatacaccg tatgaagcat
     6781 tgtatattct tcctgcatat cgcttgaaag ctggcgatac atataatcta attcattgag
     6841 ctggtacacg cttgtatgct catggacttc atcagcataa ggcaacaatt tttcaataat
     6901 gcggtaatct tgactttctg tactgacacc taacttttct tcaaactcgg caacctctat
     6961 cggcaggtcg aaccagtatg atgttgtttc ttcgccagtt gttcttttcg tttccaccag
     7021 tacccttgtt tcctgcattg ttcctcacgt cctttctgtt gtttcatcac atcttttaaa
     7081 gtctggcatg acataccaaa cacatatttt gaaaaatctt ctaactgttt ttcttcatag
     7141 tcggcaaggt tcagttgctt catttcatgc cacaccttac gcttgtaaga gggcaggtct
     7201 gaaatgtttt cacaaccaac ttttgcctgc atatctctaa aaatatcgtt cattacatca
     7261 ctccaatctg aaatttaggt aagaaaaaag cagtcaatcc taagactaac tgctttgtgt
     7321 gtatggatat gaagttgtca aactgggatt tatctggcag acttgtttat ccactcatca
     7381 agaaatttca tttgttcttc cgtgtgaaac caatgctctc cgtttctcat aacagtaagt
     7441 gttgcattag tttgttttgt aaattcagat atagttgcat aagaagtcaa gttatctttc
     7501 tcaccaaata aaatatttgt tggaattttc catgtagttg gattttcttt tacataacag
     7561 agatattccc atgagagtgt ttctccaaaa gttgttttaa tttctttttt atctcgaagt
     7621 tcatcttctg taacatttgc ccaatacatc atatctgcaa tcagtttttc catattcaca
     7681 ataggtgaaa taaaatatgc tttttctatt tgcttatctg ataaggcgtt catagcgaaa
     7741 taggcaccta tgctatttgc aactatttga acagatttgt aatgtttgca aatcaaatca
     7801 aaaagtgatg gaaattcttc ttttgcttcc catggatatt gtgcagtata gttgagtcca
     7861 accacatcac aatcactaaa aagaggtttg taatgtgaag actcctctgc atttccacct
     7921 ttaccatgta tataaataac tactttatcc ataatagttg tcctcctcaa cttcaaattt
     7981 gtcgctttct tcatatccac attatatcat tctcatatca gcaccacaat aaaattttac
     8041 gcaccgataa tcttattgaa cagctctaac agtacgtctt tcacaccgcc agcattgaat
     8101 accaatccaa cggcaacaag ggcaactacc aagaagccga taagtttaga aaactcacgt
     8161 ttgaacccta agtagatacc aataaccaca atcgccataa gcactaagct ctgggcgttg
     8221 cttaaaaacc atgtgtatat ggataggtaa gttcctgata ttataaatat ctcaaacttt
     8281 tccccccatt cacacggagc gtgcgacttt caccgcactc cgcgttccat cgtgtttata
     8341 ttgaaaagta tttatgtagt agacctatat caagtttaat tcttgattgc cagctaatac
     8401 tctatattta tttagtctta tgagctggac tttatttagc ttcaatagag ctatatattt
     8461 ttgtattgtt tcttctgttt tagcgtgtat tagacggtgt accgcctctt ttaccaaaat
     8521 atgattttta taatcgtcat ttccaccagc tccagttggt acaatatgat gacaatgtat
     8581 ttcattagct tgttgaaatt cttccagagt tatagcacat tttccatgtt gagcagaaaa
     8641 tagcgaaatt cgattgtctg cgtattctat tgtgcgattt tccaaagata atttcattaa
     8701 ctggtacatc agtaatttat caacacttag attggtgtgg ataaattttc ttccctctag
     8761 agtgtagcaa ttcgctaaat acacctttgc cattggtttt ctgtgttgga tatatccaac
     8821 agggaaaata ggttcgccct cgccagcaac ataccggagc attttagatt taccatatct
     8881 tgttgcctcc tttgtcgtaa gttgtctgcc tgtttttctc aatctgcccc ttttgtttgt
     8941 acctaatctg ttagtgaaga tagccattac agctctattc agtttcatac aatccagatt
     9001 tacattcgtt gcaatctgat aataattttg catacctaat accattgaat tataaagtct
     9061 tgtttcttct aattccgttt tcccttttga gggagaagca attttctttg cctgtgatac
     9121 cagcttgtct ttttggtttt tgaattgttt atcacttata tgtgatttaa caactaactt
     9181 ctttcttctc ggtatcattt taatcttgaa accaagaaag tcggagtatc tttttctaac
     9241 atttacaatt cttgtttttt cttgtgatac ttccaatttt aatcgttgtt caatccattg
     9301 agtgattgct attttggctt tttcagcact ttccttgtag cggcagaaaa ctctaaaatc
     9361 atctgcataa cgaaccatat acatttcctt taactttgtt ttcttcattt ctttgtaagc
     9421 gttgctttta catgggctac cgcttttatt gatatgaacc acatagtttt ttgtaacagg
     9481 attttcctgc cattggcttt ctacccaatg gtctaactca ttaagaacaa tgtttgctag
     9541 tagtggggat attattcccc cttgcggagt tcctttctct ggatatgtta ttgtaccgtt
     9601 agtcattttg attggagctt ttaatatcct ttttaaaatg aatatcaagt gcttgtcccg
     9661 tattcccata gcccatatct gttttatcag cttggaatgg ttcacgttgt caaagaaacc
     9721 cttaatatca aattcaatca cataatgtag ctggctgatt tgtaggcggt tataggttgc
     9781 ttttattgca ttttcaactg acctattagg tctgaaacca tagctgtttt cactaaattt
     9841 agcttcgcag attggttcta atacttgttt tatacattgt tgtataagcc tgtcccacat
     9901 acaaggaata cctagaggtc tggttttacc atttggtttt ggtatttctt ttcgcctcac
     9961 tggcttaggt cgatacccat gtggactgcc tgcaacaatg tatcttactt ttttgacaac
    10021 tgtttctgct ggcaatttgc caatatcttt aattattgta ccgtctgttc ctgctgtttt
    10081 actgcctgca tttgctttga tatttcgata tgccagtaat atattttctc tcgataggat
    10141 aatatccata agatgagtga aaatatctcc attcttactt ttggcatata aatcatcaaa
    10201 ggtgttttgc atttcgtaat attcagcgtg tcttaaatct tctacacaca atgtttcatt
    10261 tttattttta ggcataaggc ttcactcctt tgcttgaaat tgagtgagct tttttatctt
    10321 actcgtaatc cttattgaaa caacataata cttttctaat atatacacga cttgagggca
    10381 ttcctccatt ctcattacaa gaatttctta ggttcgccct ctgcttttca gcattagttc
    10441 aactgcttat tgttcttcgt cagtttattc tcttgatgag aacccaatac ctttcctcgt
    10501 tccgataatc ttatctatac atatatatcc ttaggtcact agctataagc ctgctggctt
    10561 gataccgcct gtaacgttat atggcatttc ataacctata ttcttacttt gccacaccag
    10621 ctacgatttc tcgtaccttt gcatttctac aaagttcaaa tttagacccg tacattcgct
    10681 gtttcgtcag ttccttatag aacattctaa ccatagatat tttatagacc cccgacctgt
    10741 tggatacgct acccgcctct ggatagtttt cgatagaaga tatatgttca tcaactatta
    10801 cggtatcttt cagccgtctt taccgagctt caaacattct gagtttcctc atacaatgct
    10861 tgtcggaggc tcagggcagg cgtttcagag cgttacttca tcattccacc tgtcagatta
    10921 tcagttctcc taataaccaa actttttcgt tcttttatta gtgccttagc ttttcactaa
    10981 ggaacgagtc gcacataaat tttgtccgaa attcatttaa ttgtcctctc tttctttgat
    11041 ttcttccata catctgtcag cttctttgcc gacagttaaa atgcacatca agactacacc
    11101 gatacccatt ccaaatgata ccagcaataa gtctttcaat aattcccaca ttttttcatc
    11161 actcctttac gttggcaacc atttcttcaa ccgtggctgt ctgttgcttg attatctggt
    11221 agtgtcgttc ggtcagtttt gcctgctttt caatcgtttc cagatactcc gtctgattgt
    11281 ccttgtctat ctgcttcaac atcttcaagg tcggtgcaac ctgcctttgt agccagttga
    11341 gtgtacgctc cagcgtgtaa ggttctggct gtgttgtcag ctttacgggc ggtcgcccct
    11401 cgccaatgaa ccaagcccag cgtacattgg ttttccagtc gctttttcgc ttgtctggtt
    11461 ctttatcagc gaagcggata tattgattga taatagaaaa tgctgtcagt tctgcgtcat
    11521 aatgtgtcag cagttccaca acagcataat aggcacgttc atttttcaaa cgtatctcaa
    11581 atctattttt tatggggact tcctcaatcg gtatgccaag tttggcgtac tgttcgtaat
    11641 tcttttcata acagcagaag tacacttcac ttgaaaatga accaatatac agcgtgtgtc
    11701 ccataccgta tttatccgtt tcattatgct ttaccagctc tccagaacca tagcttttga
    11761 aagaacggaa tttagaaata cattcctcat tctcgcattt tcgtatcagc tctggaatat
    11821 caagtaagcc cgttcggtca ttgattgcca aatcaagacg cttcattaca ccgccctcaa
    11881 ccaatgcgtc cgtgaagaag tcataccaac ttcgctcttg tgccagcaga tagctttcaa
    11941 actggcgaca gcctttgcct ttcagttcca gcaggacacc tttttcttcg tcctgcgata
    12001 catacacaaa cacatctccg atatggtaat gctcggtgta tttgtaatgt ccgtaatctt
    12061 cgtgaagcat atagccgatg ttgagcttca atatatcctt gatgatatgc tttatatcca
    12121 ttgtgggaaa acggatacgc acataatcaa acagcaggaa catgggattg ttgggattga
    12181 atttttccac cgcttctaaa agcgtatgtt tcatgtcctc ggtcagcgtt acctttcctg
    12241 cttcgatacg gctcaaatgt tcacggctga tatttgccat gattgcaagc cttgtctgcg
    12301 atacgccgta tgcacagcgt ttttctttca aggcggttaa aaaatcagtt tcattcagtt
    12361 gtcatacccc caatcgtgat aattttgtga tattgtgata atttcaaggc tttatcacaa
    12421 aaggcgaaat gtacgaaaaa cccttatttc atgcggtctt gcaaaggttt ttgtggaagt
    12481 ttccgccgta attgtgcccc tctgttagat aatgaggggc ttgtatctgc tggcgtggct
    12541 aacgccacac cagcaacggc tagtccgtgc cgtcgccttt cgcttcgcac gtcgccgtcc
    12601 cgtcctgcct tgcgtatgca agtgagccta tggtctgcaa aaaatcatgt ccttttggaa
    12661 ctaagggtgt gtaaaattca cttatgacgc ttgttcccac atcacaatag ccacgtccct
    12721 tgatacgctt ctggaaaaac tgttttttca catcagaact gaacagcata ccgtaaccta
    12781 attcgctgat acgccccaag ccaacacgga aattaaaatt atctctgata ccgtccgaga
    12841 aatattttgc gtctggacgc tggcaggcaa caataaggaa atatcctgct tgtcgtccta
    12901 acatgacgat tttctttagc tggctaagta ggctcacact ttcttttgtc cccagcattt
    12961 caaaaaacgc cacatattca tcaaagataa gaaagcaggg cggtaaacca agataggcat
    13021 agttttcgcc cgttttatag tctgggtgtc gcttcatttc ttcgcttctt tgtaccatgc
    13081 cctcataaaa ggcattgaca caatcaatca tttcttcttt ggtgtgatac acatttccca
    13141 ttaccgtacc taaatctgca aggtcagcgt tcttcgggtc taatatgtaa agaacagcgt
    13201 tcgtatgtaa cagagcttca ataagcgtca gcagaaaata cgttttaccg ccaccagtcc
    13261 caccagcaat cagagcgtga gggagtgcgt catattccca tacaagattt ttcatcagcc
    13321 taagacagcc gttttctgct ttcacttcat caatggtaat gcggttcgct atcatatcat
    13381 aaagcatggt gtattcgata taaccgtcat gtaacgtctt gtcggtcagc tcacaataca
    13441 agccactttc caatttatcc tctaaccgta agagctggtc ttgatatttt cctaatgtaa
    13501 tttcacagcg gatatgaagc agtccttttt ccatttgata ataaatcttt ggaaaccaga
    13561 tgattttttc ccttgatctg ctttgcaggt cagtaaaaaa accgctgtct tgtacggtgt
    13621 ctgcttcata ccacttgttt tccagtatca ttcgtgccag cttttgacgg tgcaggagct
    13681 tcttgaaact gtcataacag aagcggtaat acagaaaagc gaccaatgca caaacaccag
    13741 tcgctatcag tagggttatg aagttgtaag tggaaagcgt caaaccgttg tctaataagc
    13801 tgaaatgctc ccaatcggta cgcatgagct gtttcatatt cagtagcagg ataaccgcaa
    13861 tgaatacaaa cagaagcgtc cctatggaaa agtggtagac aaggttcttg tcgctggctc
    13921 tgatacggtg tcctttgttt aaaatcattt tcataaatca atcactcctt tctgggtacg
    13981 aaaaaagcag tcaatcctaa gactaactgc tttgtgtgta tggatatgaa gttgtcaaac
    14041 tgggatttat tgttcttaac ttttctgtcg ttataaagct gacttacgca cattaacctc
    14101 tgcacagacc agcccagcta tatgctccgc tgtttcattt gtcagtacaa agagcattcc
    14161 gtcattacgg cattttttat gtgtgtcgcc attcaaggca taaacaaaac cttttacaca
    14221 ggtatcacta caggcattag ggtcaatcag tctcttgcag tcacgagaag tagtcatttt
    14281 cttttgcata tcagcaggaa gcgaagcgat tatatcctca tactttccag cgttgtctcc
    14341 gtaaatccgt gcaaaaacac ctgtcttgcg aaacacccaa ttcattactg ttttcttctc
    14401 tagttggtat gaagcggtgt aaccgctttt tgcttccttt attacaaggt cacagccctg
    14461 ctcaataagt ttggtattaa gtttctcgac aaacgcttgg tgttctggcg cgattgccga
    14521 tagaaattct ttgaatgaac ctttttcttt tgccattatc tttttcctcc acaaatttca
    14581 atttatccgt ttcttcatac acatattata acagttgctt atatccgcta caatgaaaat
    14641 ctagtttgcc agcttatttc tttggttgcc cctgcggtgc atggttctgt ggattgccta
    14701 cttgaccgcc tttattcttc aagataatat cgtcagcctt gatataccag tccacattga
    14761 cacctcgata gctggcgttt gctaccgtat cggctacggg attgacaagc tctacctctg
    14821 cattatacgg atagtccttg acgggaatat cagcaggaat tgatacttgt atcatacgtt
    14881 cctgcacact acatttcaaa tcataggttc ttcgtgtgat ttcttcactt gttgaaccgt
    14941 cgtcattttc tgctcgcacc tcatgtctga gggcagagaa cttcaataca ccgaaagttt
    15001 tttccttgtc taatacaata ccgtttgcta atctcataat catttacccc cttacttgct
    15061 ttcctcggct ggcaacatat catcagccgt taagatatag tttgtgaacc ctcgtccttt
    15121 gaccgcttta ccgtctgcgg taatctttgg attgataagt tttactcgct gttctggttt
    15181 gaaatgcttt tcgcctgctt tcgctggaat ggttacgata atatcatcag ccctttgtac
    15241 gtcagagtac aagttgtagc ttcttgatac gggcgtaaag cgtccgttaa tgcgttgctg
    15301 ttcaatcttg ttttcgccag caaattccaa gtttccaaaa gtctgtgcca tgttcggcac
    15361 tacatatttt aattccatag tgttttacct cgtttctttc ttctttttta agtttgttga
    15421 ttgtgctttc ttattctggg aattagggaa ataccagtaa tcccccagat agtaaagggt
    15481 aaatcaatgc ttcgcaccct tgactatccg tgtgcttgca ggttgttggc agacaagcca
    15541 gaataagtaa acgtctgccg tttgacagct tcggcagaga gcgtgatttt tcttttctca
    15601 taccgttacc gccttatgat ttcttcattt tacggcgttt gtaaagatat gttccagcca
    15661 gtccaccagc agatgtaagc aacattacca gaaatgccat gacatttgtg ttgtcgcccg
    15721 ttttaggact gtctgttggt ctgctcggtt tttctggtgt tgttggcgtt tcgggtttct
    15781 ctgggacttc tggtttttcc ttaaaagtaa tcgtctgtcc ctcgtcctca atgtccttat
    15841 gctcagtaac ttttttcggc tcgtttggat tgcttaaatc atacaattct tcaaaagtta
    15901 caagctgttt gccgtcaaga gaagtagcgt caaaggtaaa ggtaacttcc actttcatag
    15961 tttcgctgtc agcgataaac gtataatcac tttccacacg ttttccattt ataagaagct
    16021 ctgcattttc ttctttcaac atctgccagc ccacaagttt atactttgta ccaacttcca
    16081 agccctctaa ggttacagta tcaatgattg ttacgtcttt tcctgcttca agctctttgt
    16141 taccgtcctt atcggtagca gttgtatgta ttttgatgat acgttctgta ataagtaccg
    16201 tctgcccgtc gtcctcaata tctttatgct ccgcaacttt catgggttcg tctggattgc
    16261 ttaagtcgta taattcctcg aatgttacca gatttttacc gccaagctcg gacgcattga
    16321 atgtataaga aatttccact ttcatttctt catcatcagc aacaaaggta taatcgcttt
    16381 ctacacgttt cccgtcaatg ataatctcgg cgttttcttc ctttaacatc tgccagcctt
    16441 ttaactgata cttagtgcct tttgtaagtc cgtctaattt gacagtatca acgattgtta
    16501 cctcttttcc tgcaaggatt gtcttttcgc cgtctttact ggttgctgtg gtatggatag
    16561 tgatttcctt ttcgtattca tcagtcaaag tccctaaatc aatcacaaga ttatttcttg
    16621 ataccacgat ttcaaatggt gggataagtt ccatgccttt gttgctgtca gagcgtaatt
    16681 cttcaataat gtaagtatcg taaggcaacg cacctttgct gtcgtctggt tcagaagttc
    16741 caaaccatac accgtcctcg cttgttttgc cagcgttggt attgtgcttg tgcgaagccc
    16801 attttgaaga agtggagaat tgcccattat cgtcagttac cacaacatga ctttcgcccg
    16861 ttgtcttgct tgtgatccta aagggaacat cagcaagacg cttgtgtgtg cctgcaccga
    16921 ttttcacgcc ctcaatatcc ccacgcttaa tctgattgta gatagagtgg gcttcgtctg
    16981 ttaagtccac gatttttccg ttttctgtga ttgtaaaatc aatcggcttc gcaccgtctg
    17041 tcaagtaacc gtttggagct tgtttctcaa cgatacggaa attcccgtaa ggcaaaaggt
    17101 cagcagaagt agaagcgata ccctcaatat ccgtatggat tgtttttaca acttcgtttt
    17161 tcttgtatat cttgccctca accaataccg cattatcatt taaggaaatg atgtcaaagg
    17221 cagtatcttt caaagtggca cttccttgtg gtttggtatc tttggtttct aaatctcgtt
    17281 tctgaatttt gacaccgcca cgaatgactt tatctgatac ggaaaactgg ttacttccag
    17341 ttaatacggc aaggtcgccg tcctcggtaa tctgcgtaag gtataaggct tttatctgtt
    17401 cttcactacc attcgcctgc atatatgcac cgtctagtaa gtagcccgtt ggcgatttcg
    17461 tttcttcgac cgttagtgta ccaagaggta aaaacgcttt tccgtcctgc atatagaagc
    17521 tatcgccaga taccttgtat gcgtctgcta attttgtgat gtaatgggtt gtcccgttac
    17581 tgtctgtttc agcggttgtc tttgtaaccc atgtacgagt agcttcggca ggaagattgt
    17641 ctttattgta gaagcctgca taatatttcc atgtaaactc cgccccctct aaagaagcgt
    17701 tcccttgtgg gttggctttc tgtgtttcca tgtcaatttt aaaaagctca atcaaagtat
    17761 cggtaacttt tggcgtatca gatactttca aagtcgctgt ctttccagct tcgactttta
    17821 aggaatacac cgtagtatca actttatacc ccaatggtgc agataattct ttgatatata
    17881 cggtagtagc tttgacttct gcaacctctg tatttccgct gtcgtcagtc gtaagggtag
    17941 caatctgttt ggtgcaatcc ttatcggaaa agacaccgta agtcgcacca gcgatagagt
    18001 aatttccgtt accgttagta atgctggtat tattggaagt cttttgaagt ttggcatttc
    18061 cgacatttaa cttcgcccag aattgcccta attcctgccc ctcgccagag tagatataac
    18121 cgccgcattc atagcgtcct ttgttttctt tcacaaaggc ttttgcacca gcaaacactt
    18181 catcttgtgt tgcctttggt atttcatcat aagaagctcg cacgttatca cattgccagc
    18241 ctaagtgaac actcaatctt tgccacacaa caagctgtct taaaaggtag gcgtgatttt
    18301 tgcttatccc attgtggttg tcggtgtatt gcttcacata ttcgatagat aaggcaacat
    18361 cacttatctg gtcggcactc atacgggagc ttgcgtcagc tctggtcttg tagccgttct
    18421 taaaatctgt gttaatatcg atacaataag cgtcctcgcc ctcaacggtc aaatgcccct
    18481 cgttaaaggt agaaccaatc gaaccgtcat tcattacttt ttcaacgata ccgacacgtt
    18541 cttcactttc cgtccagtat tgcttgcttt ctgcatggac aagtgtagtc ggtaaagcag
    18601 taacgacagt cgcaaaggca agtacgcccg tacacaatcg tttcaatatc tttttcataa
    18661 ttctaaatgc tcctttcatt ttgggtaata aaatagccgt ccataatgaa cggctggaaa
    18721 tagaaaagga acgtcaattt aggcgttcca tagtctatga aatattcaat ttttttcttg
    18781 cttcaatact gatgtgccgt accatatctt tgcatatcta ggaaaacttg cgtatcaagg
    18841 ctcattcatt ggtagtaaat tagtagtaaa ttgatgtgtt tgtttccttg aaaatgctga
    18901 tagatactat gttttagcgg ataatcaact ttttagtatg ttttttgcat aaaaattgag
    18961 tagatagtag ttttattgta cttaatgatt gtatttaagt gattagattt gaaattgaat
    19021 aatgtagata ataaaaaaat attaacagaa cttaataaac tccattaata tttttttatt
    19081 atttttgaaa ctgtgataat tatcacggaa gcatagaaaa taaagtgtta agatttaaac
    19141 aagatagtaa gtaagtaaaa ctaaaatata agaaaggtga taaaaatgaa actaaaatta
    19201 gattcatcta agttcaatga aggtctgaat tatctaaaaa aagactacaa gatatttgca
    19261 ccagttttaa taccatttaa aggtactttt tcagatactg atgttataag atacaaggag
    19321 gtaaacgctt ttgaagaaat ggaatttgct aagaaagcaa acttttcacc aaaagatgtt
    19381 gtacttccca taaatcaagt attgttttat tttactgaaa aagagtttaa ggaaagcgat
    19441 ttagacaaca agaaaatttt aattttctta agagcttgtg acataaatgg tataaaaaga
    19501 cttgatgaaa tatatctaaa taatggtgaa gaaaaagatt atttttataa aaatatcaga
    19561 gataaagtaa agtttgcact tgtagggtgt aaggaaagtt ttagaaactg tttttgtgtg
    19621 agcatggatt ctaacaaaac tgataattat tcaattggat taaatataga aggagataca
    19681 ctgtacttag atataaagga tagtgaattt gaagtattta atggagaagc aactgaattt
    19741 aatgttgatt atgtaactga taatttaata tctgtagatg tgccagagaa tattgattca
    19801 aatgaaatga taggaaattc aatatgggat gaatatgata caagatgtat aggatgtggt
    19861 agatgtaatt ttgtatgtcc aacatgttca tgttttacaa tgcaggatat tttctataag
    19921 gaaaacgaaa atgtaggtga gagaagaagg gtttgggctt cttgtcaagt agatggatat
    19981 acagatatag caggtggaaa ttcttttaga aaaaaacaag gcgaaagaat gagatttaaa
    20041 actatgcata aaatacatga ttttaaaaaa agattcggat atcatatgtg tgttggctgt
    20101 ggaagatgtg atgatgcctg tccgcaatac atatcatttt caaagtgtat agaaaaaata
    20161 aatgatttag taattagtaa ggaggaagta taaatgaacc catatatacc agtaagtgca
    20221 aaaatactag atatagttaa acatactgat aaagaatgga cttttagagt aaactgcaat
    20281 acaaaaggtg tattgcctgg aaagttttat gagatatcta tacctaaata cggtgaaagt
    20341 ccaatatcag tatcaggata tggagaggat tatattgact ttacaataag aaatgtagga
    20401 aaagtaacta gcgagttgtt taattataaa gaaggagatt ctttttttat aagaggtcct
    20461 tatggaaatg gatttgatgt atccctttat gaaggaagag aaattgtagt agtggcagga
    20521 ggaagtgcat tagcccctgt aagaggaata gttgaatatt tttataataa taaagaaaaa
    20581 tgcgaaaagt ttaaattaat agtaggattt aaatcaccaa aagatatttt atttgcagat
    20641 gatttaaaga gatggagtaa aaaacttgat attttagtta ctgtagatgg agcagaggaa
    20701 ggatatagtg gtaatgtagg attagtaaca aaatacatac cagaattgga tatgaaagat
    20761 ataaacaata cttctatagt tgtagttgga cctccaatga tgatgaaatt tacagtagaa
    20821 gaatttttaa aaagagattt agatgaaaag aacatttggg tttcttatga aagaaaaatg
    20881 tgttgtggaa taggtaaatg tggacattgt aaaatggatg atacttacat atgtttagat
    20941 ggaccagtat ttgattattc atatgctaaa aatcttattg attagggggt gaagtttgtg
    21001 ataagagatt taaataccaa aaaagtaatg aaaaatgctt atagaattac aaaaacaaaa
    21061 tatgagactt ctttaagagt tagagtacct ggaggtctta ttgaccctga gagtttagct
    21121 gtagtttcta aaattgcaag tgagtacggc aatggacagg tgcacataac tacaagacag
    21181 gggtttgaaa tattaggaat aaatatggaa gatatggaag aaataaataa aattatacaa
    21241 cctgttattg aaaaaatgaa tataaatcaa agtgagaaaa acgcgggtta tccagcagca
    21301 ggtacaagaa atatagcagc ttgtatagga aataaagtct gtccaaaggc tcaatataat
    21361 acaactgaat ttgctaaaag aatagaaaaa gctatatttc caaatgattt acattttaaa
    21421 gtggctttaa ctggttgtcc aaatgattgt ataaaagcta gaatgaatga ctttggaatt
    21481 attggtatgg ctttaccaat atatgaaaag gatagatgtg taaattgtgg agcatgtgta
    21541 aaaaaatgtt ctaagatatc tgttggagca ttaaaaacag aaaataataa ggtggtaaga
    21601 gataaagata agtgtatagg ctgtggagag tgtgtactaa attgcccaac aaatgcatgg
    21661 actagagatg agaaaaaata ctatagatta gctataatgg gtagaacagg aaagaaaaac
    21721 cctagactag cagaagattt tcttttatgg gtagatgagg acagcataat aaagataata
    21781 cttaatactt atagatatgt agaaaagtat atagataaag atgcaccagg aggaaaagaa
    21841 catataggat atataattga tagaacagga tttatggaat ttaaaaaatg ggcacttgaa
    21901 ggtgtagagc ttccagaaat aactaaaatg tacgaaaaca tatactggag tggaattaaa
    21961 tatctataaa ttgagtaaaa catatatttg aataaattaa aatttaatag aaggaagagg
    22021 gagttatgca taaagaaact ttggataaat taactaatgc agctataaat aagataaatt
    22081 tattaaacac aagtaaagta aaatatttgg tatcttctgc gtttgcagga ctttatgtag
    22141 gtataggtat acttttgata tttactattg gaggactttt gactgatgca ggttcaccta
    22201 tgactaagat agtaatgggg ttatcatttg caatagcact aagtttggtt ataatgacgg
    22261 gaacagagtt atttactgga aacaatatgg ttatgtctgc tggtatgtta aataaaggtg
    22321 taagtataaa agatacttct aaaatatggg catacagttg ggtaggtaat ttaatcggag
    22381 ctttggtttt aggcattata tttgtaggaa ctggtctagt agacaaagga cctgtagctg
    22441 aattttttgc aaatacagca gctagtaaag catccatgcc atttacagct cttttcttta
    22501 gagggatttt atgtaatatt ctagtatgtg tatcagtgtt gtgttcgttt agaactaata
    22561 gtgatactgc aaaaataatt atgatattct tatgtttatt tgcttttata acatcaggtt
    22621 ttgagcatag tgtagcaaat atgacaattt atagtgtttc attattttca ccaacaataa
    22681 gtacagttac tattggagga gctatttata atttagtagc tgtaacactt ggaaatattg
    22741 ttggaggagc tttatttatg ggtcttggaa cttatatatt aggtaaagaa aagcttaatt
    22801 aaatttattg tataaattat aactctcttg ataaatacat ttttagtatt gttgagagag
    22861 ttatttttat atttttgaaa aaatatttga aacaaaaaaa atatgggagc atacgaaaat
    22921 atatcggtta tatatataaa aaatacgttt atatatgaat tatatagttc taaatataaa
    22981 aaaagttaaa gtcctaccgt aaaaaagcgc attttgaata agataacaaa tatgataaaa
    23041 ttggtcttgt tgaaattttt aggggggata tgatgacaaa gaaactagta atatcaaaaa
    23101 caaagttgtt tatttttata atagcattta tttctatatt tatatattta tttggaagta
    23161 aaaatacact tataggtgta ggtatagtaa cagctatgct aacactcttg gaaagagatt
    23221 taactatttc tccaattaaa aatttactga agtatttagc aataaatata atcttaggaa
    23281 tactatcatt ttttgcagta caaaatatgt atttaggagt tttattaaat tttatagcat
    23341 tatttataat agggtatgta tttagttatg acttaaaaag agcagtatat gtaccctttg
    23401 gtctaatgta tatatttatg gtaagtatac ctgtaggaaa aagtgaattt cctatgagat
    23461 tgtcggcttt agctgttgga gcagttataa taatgattgc tcaatttgtt atgaatagaa
    23521 ataggatgaa aaatgtcgga gataaagaac tgatatctat atgtgatgaa ttattagaaa
    23581 aaattagcct tttaaaaaat actattaatg atgatagttc tataattaaa tctagtatga
    23641 gaaaaataga tagctgtaat tatagaataa attctatatc taaaaatttg aaaatggtta
    23701 tttttgataa tagaaaagat gacttttata tcagtattag aggtatagac ataatgaata
    23761 ttttattttc attagaaaga ataagtttaa tcctggaaag atacaaaaag gatagtaaag
    23821 aatttgaaga tgaagatata aaaaatattt tagaagaaag tagcataaat agtaaatcag
    23881 atgttttagt tgtagcaacg aaagaaataa actatattaa gatgtgttta gaaaataaag
    23941 ataccataac tgataaagag atattaggat ttagagatta tgttattgct caggatacta
    24001 aaaatataaa tttaaaagaa atttatagtg ttttagaaaa tttatatgag tttttagtag
    24061 attataaaaa agtaaaatca agagacgaaa agaaagctga aagaaaatca aagatacctc
    24121 atgaatttaa acgactatca atatataaga aaaactttaa tttaaattct ataagatttt
    24181 cttatgcagt aaagattgct ttagctacag ctgtagcggg ttttattatg gattatttcc
    24241 atttaagaga tggaagatgg ataatgttaa ctgtcttttc attaacacaa ccttacgcag
    24301 aaaattgtat acaaaggtct agaaaaagaa tagaaggtac atttatagga gctgtaatat
    24361 ttattgtatt attttcaata ataaaagata gtacattaag gtcgttaata gttcttttag
    24421 cagggtatat aaactcttat gtagttgatt atagaaaatt aatggtatgt gtaactgttt
    24481 cagctcttgg ttcagcagta gttatgggag acccaaatgt tttaacaata agcaggattt
    24541 catatgtagc attaggagca attattgcat taatagtaaa taagtttata ttaccatatg
    24601 atgctaaaac aggctatcaa catgttatag aaatgtataa aggaatagtt aaaaatataa
    24661 ttgatgaagt caataagtct atagaaaatg tagctgatgt ttactatata aaaaatttat
    24721 tattgatacc atcacttatt gaagatagac ttatgttgat aaatgctata tataaagata
    24781 aacatcaaga ggatttttta gaaaatcaga aattactaat aagtaatatg tataatctat
    24841 atataaatgt taataaaaat aaaataaaag atgaagatgt tgaaaaaata ttgagagata
    24901 ccaattatat ttcaaattac aatgtagata agtatgatga aggaagaagt gtaattttag
    24961 agagtattgt aaatactaaa agtctaggtg acaagataat atgtctaaac ttactgcaaa
    25021 ctctaaatgg tgtcaaagaa atgtatagaa taagtaatat aactaaagtt tcaataaaag
    25081 aagcagctta gatattaaat tatagaatag ccatatattt aaaataaagt atatggctat
    25141 ttgttttata aaatactaaa agaaaataat atataaaaaa caaaaaacat atatttatga
    25201 attttatatg aaaatagtta aaatattgat ttttttcatt ggttgaaaaa acaatttact
    25261 taggataaac tataatagta ttcaaatatt atgggggtta gatttatgag taaagatgta
    25321 aaggtaaagc gtaataagaa atatgataca tttagtataa ctatggaact tgagagacct
    25381 ttactttatg atgaggttga gaacaaagat gataaaaata ataatagaaa atttaacata
    25441 aagaataaga tatttaaggg aataggtata ctatttgtat tgattttgct tggctttttc
    25501 atatggataa ataaaacata tgaacctcaa aagctggcaa aagaagctct agtcagcaat
    25561 agtaaagttg aagtaactgt aaatgaaaac atttcattta caccaaaagg aagaaaagtt
    25621 tctaaaggtt taattttgta tccaggagct aaagtagaaa tagaatctta tgctccactt
    25681 gcaagaaaaa tagcagaaag tggatatgag gttgtaatcg taaagatgcc acttaatcta
    25741 gctatacttg gaactaataa ggcacagaaa gttatggatt cttacaacaa catagaacat
    25801 tgggtaatag gaggtcattc attaggagga gttgcagcat caaattttgc acgtgataat
    25861 aaattaattg aaggcgttgt atttttagct tcttatccaa tgggtgatga gttgaaagaa
    25921 ttaggaaaaa aagttatatc tatatggggg agcaaagatg gagtagtcaa ttttaaaagt
    25981 cttgtagaat caaaacaaaa acttcctgat gatacaactt atgttgagat tgaaggagga
    26041 aatcatgcac aatttggaga ttatggaaaa caaaagggag ataatgatgc tattataagt
    26101 caagaaaaac aacttaatat tactgccaat agtatcatta aatttttaaa gaatatatct
    26161 taaactaaaa tttaaaaaat ataattttat aagttattat ttaaagtaaa tattaataat
    26221 ataatagaat gaatatttta ttacaagggg gattttaata tggaatacga tatgccactt
    26281 tacagaccac caagtgaggc ttatagtctt ataatacaag tcacactagg atgttctcat
    26341 aacaaatgta cgttttgtag tatgtacaaa tctaaaaaat ttaaaataaa gccactagaa
    26401 gtaataaaaa atgaaattga tatatttaga agatattata agcatgtaga aagaatattc
    26461 ttagcagatg gagatgcatt gataatacca atggagaaat taagagatat tatcttatat
    26521 ataaaagaag tttttccaga gtgtgaaaga attactatgt acggctctcc taaatctata
    26581 gagaaaaaat ctgatgatga acttaaagaa ttaagaaaac ttggtgttaa aatgatttat
    26641 ctagggcttg aaagtggaag caatgaagta ttagaagata ttaagaaagg ttttagtagt
    26701 gaacaattaa taaaagttgg tagaaaagtt aaaaaaagtg ggattaaatt atcagccact
    26761 gtaatagcag gtcttggagg aactaaaaaa acacatcagc atgcagttga tacaggaaaa
    26821 atgttaggag caatttcacc agattatgtg ggtgtactta gtttaatggt agaacctaat
    26881 actgaacttt acgatttact taaggcagga gagtttacaa tactagagga taaggctgtg
    26941 ttacaagaaa ttaaagagat gataaagaat atagatgcta atgaaaaaat tgtatttaga
    27001 agtaatcatg catcaaatta tgctaattta aaaggtgtat tacctgatga taaacaacgt
    27061 ctaattgatg aaattgatta ctatctaagt aaccaaaagc taaaaagaga agaatataga
    27121 agactatagt ttttgatata tcaatctata ttgataggta aatactaaga ttatttagat
    27181 acaaaaaatc atgctacatt aatagttgct tactattgat gtagcatgat ttttttatta
    27241 aaaaacaaaa tggtatattc atatataata tatcaataat aatgaaaatg tgaactagtt
    27301 taatagaatt ttacctatta taataaaaac aggaatttaa ataataaaaa gtaaagtaac
    27361 tttttattaa gtaactttac tttttatttt atttaatgtt gtattaaatt tatatatatt
    27421 aaaataatat acaaattttg aaaaagctct tataaaatga ttattaaagt aaagtttaat
    27481 ataaataaat tataaaatat taaactaatt tatcaatatt tcctgattga atatgcttaa
    27541 tgtttgattg aattttttta tatggccaat cccaccattt aatttctaat aattttgaaa
    27601 ttgtatcatt attaaatctc ttttttataa tttttgcagg tactcctcca acaatagaat
    27661 acggtggaac gtcattagta actactgctc ttgttccaat aattgcacca tcaccaatat
    27721 ttacacctga cataattata gaatcataac ctatccacac atcatttcca attataatgt
    27781 tacccttatt gtcccaagcg tccgttatat ggttgatatc gagtttccat tcttcataaa
    27841 agatgggaaa agtatatgtg gattttgatt tcattgagtg atttccacta gtcattagaa
    27901 attttgcatt acatgctatt gagcaaaact taccaattat taatttatca ttgtttatag
    27961 ggtaatgata caatacattg tttttctcaa aatctcttgg gtcattataa aagtcattat
    28021 atattgtgta atctccaact tctatatttt cccttgttat tacattttta agatatacag
    28081 tttgataatc attacttctt ggatatacct tatttgaact tggaatagtc atatttaaat
    28141 ctctccttta atatatatta tgtttttgac tattccacta ctacaataca tctcttcaca
    28201 gaatcactcc ttgttaagaa tcttcataag ataatggcgt ccttttttag tgttaagtcg
    28261 gatattcttt cttagtccat tctttttagc gtataactga caaactgtca ttttcttacc
    28321 aatagtgtca atgctatcat caaggtcatt ttgtattgct ttttccttat cccttaatga
    28381 tatatcatca cgttttccct ttgggggttt atcagtaggt acaagtttcc aagagtaaac
    28441 aaattgtggt ttaccaaatg aatatacata cttatatgcg tatcttccat cttttctctg
    28501 gctctctcca gttcttaaaa ttctatcttt attatctcgt cttttttcag ttgacatgat
    28561 aagaagctcc tctcaataac cgaattgagc ttgatacgtc atatgaatat tataacatat
    28621 aaggctcaat ttcattagat aaagtcaagt gtatcaacga ttttctcaaa ttgttttctc
    28681 tttatttgaa tgcgattgct attcataatg acccagccag aatcaaggtt ttcatcagca
    28741 agtttacgta attttttctc tccaatacga aagtattttg acgcttcttc aatcgtcaag
    28801 gtatattttt cccaaatagg tacatcagta ttgttcatag aatgtatcac tcctttctat
    28861 gttcgtagtt cagaagtaga tagatggctt tggtaagctc cattggtatt ttaaccaatc
    28921 ccattcttat gggcagaacc gtgtcataca ggtgtatcat tatactgtgg tacaggttac
    28981 ggcaaaacaa ccgttccaaa ggttattcac aaatcactat atagtagctc tcttgattaa
    29041 ggtcatggcg tatagtattt gttggctatc tgtatcacaa acgtatctat ctactttatt
    29101 cagttgtcaa agaacagtcg gcattgattt gtctataaaa aatcacaata gcttatgggc
    29161 ttttatagac aaataattag tgattctcca tgtaccaaat agcttgatac atggagagag
    29221 ggcgagatta agacaaatta aatctcaaaa tagcagtgat tagttttgtt cctagcattc
    29281 gctttgtttc ttcttctaca tgaatatagg gatttccgta ttcatcaaat aaagttttct
    29341 gtgaaagacg aatgatatag tgttcaaaat gtttaagtat tccgtttatt gcatttacat
    29401 cgccatgtac agcaagtaaa ataattgtaa aaggtaatac ttctttagta ggtttcattt
    29461 caatgttcct ccatgagttc tttcaactct tttagtgcat ttgttttgtg ataatgtatg
    29521 gtattttctg ctaaatccat caatgttgct atgtcagcat ttgtcatgtt gagaaagaat
    29581 gaaagtagaa ttatatcttg ttttcttttt gtaagttttc cgattgcttg tgccattaat
    29641 acatcttcaa ttcgtatgtt gtagtcgtaa gaaaccatac agatgtagtt agaatcataa
    29701 tcatctaaaa tactcaattt acttaattct gcaagtgaca aagattcgag agataccaaa
    29761 taatcatttc tgcgtttatt ttcgtcataa atatctctag catagttacg caaaactgtt
    29821 ttacagaaac tgtcgaattg atgttcaatt gtcttttcat caaccataag atttcaccct
    29881 ctttccatcg caaggagtta aataaccttg cttactccta tcacggtggg agggcgttaa
    29941 atcttcgata aatttcaaaa aaaagagaaa aaaatacaca ctctgcctat agggcaaaaa
    30001 tgtgtattct ttaaaacgta tttaatttat attggttgta agccaccacc atattgatgt
    30061 atggaaatat gcattggcta gcttttcata aaaatacaaa gatgcataag aaattaatag
    30121 aggggaaaaa cttattctgt tattaaaaag taggttaatt ctaataaatg ctattgcggt
    30181 cgaattaaac tatatacatg gcaatcaatc atgtttctat tctttattat gaattgtgac
    30241 agtattcttc aatcttaaaa ccacattttt ccaatacttt acttgaccct ctattgtagg
    30301 aatatacatt agcatgaatt ttgttaaggt tgtatttttc aaaggcaatt ttgcatattt
    30361 cttttactgc atttgttgct atccctttgc cccaatagtt cctagcaagc cagtaaccta
    30421 attcagcatt tttattaata gaggtatctt cttcaaatgt taggctgata cttccaatag
    30481 ccctttcttg ataaaaaata gcatagctaa gttgtttaac atctggagtt gccaaacaaa
    30541 aactaacaaa atctctagcg ttttgctccc tataagggtg ggggaagttg tcacgaagat
    30601 tctttgcaat ttctttatca ttcgcaaact caactaaatc ggtaatataa gcataattcc
    30661 actctataag ttccattttt cttctccttt tatgaaacag ctacaagaaa tctttctaag
    30721 ccaataccac ttatcaaaca gcgttctttt ttatttccaa gcatttgata agtccagtca
    30781 acaaatccac cgtcaactat ttcaacggtt tcatttcctt gtttgaccga taatttgaaa
    30841 ttgatacctt tataataatt attatcaaca tcatcattat gaattgataa ctctatatta
    30901 ggaaaaataa gtttgatagt ttcaaccatt ctgtcgaaaa atccgtcatt atctttgtat
    30961 ccgtttcttt tgcgtagaat aatagagaaa cgaacatttt ctatttcact taatatatct
    31021 ttataatagt ttaagtgctt ttctaacaac attttttcac aagtgtaaga ccctgtatct
    31081 ataccagatg aaaccataca atataagcca aaatgtgaat ataaaccttt acctacaaaa
    31141 ttttgtgctc ttattaccct agcagtagta gccatgtgga taggtatggt attattttct
    31201 tcttttcttt ttaatttatc agcaataatt attgccaaca tatttgaggg gtctgcaagt
    31261 gtttctgtcc ctcgtagagc acttactaca ttatattgat ctacggagcc aaaaacgcta
    31321 cagctaccta atggagcaga gggagaaagc ataatcgtct tgatatccat attgaatgct
    31381 tttctcaata attttgactc caaagcatga atttcttctg gatctatacc gctagggata
    31441 gtaaatctat ttagttggta tgactttaga atatcagttg cagttaaagt atttgcttgt
    31501 ctatcaaaca cttctaatag aagtgagttc aaatctgatt ttgatagagc tgataactta
    31561 tcaactatat tcttatcccc aattttattt gtaattctac ttaaaatatc actcatagtt
    31621 atcctccata ctgttacttt caaaataatt atagaatggt tacaacaact aatctaggtc
    31681 atttgaccaa aaatgaaaaa acactgaata tttactattc aatatttttt tactttgggt
    31741 ttatttgatt aaatcttgaa aatactgtaa tgcaaccctt tcagcttcaa cctttcggac
    31801 aataggactt tgtgataatt cctctcttaa ctcaagatac aggttgatag tagcctgcaa
    31861 tgcattaaca cagcttttat attcatggtt agcaaccgtc ttttctaatg cttcaatata
    31921 ttggggtgcg tccaattcaa tttttctaac tccacgaggt aatttaccta agagaagctg
    31981 tattaatggg gcaagtactg attgtcttaa aaaagataaa aaatcaattg tttcaaatag
    32041 ttctctacgt ccaatcttag tgcaagcgta atgaacccaa atccaaaatc tatcttcaat
    32101 ccactgtaag tctggagttg ggaagtatgc ttctttttta gtataacacc tctcactttc
    32161 ttttattagc tatcaatcca acataattat gcataggagt attatataat gaaaaaataa
    32221 aaaaaaatag gtcacttgac ccgaaataaa aaagcattga ataattatta ttcaatactt
    32281 ttttgaaatt atacttatgt tgtaggaaga ttctttaata cctctcgctg attaattaca
    32341 ctaaatcttt tgttttcata agcaattaga tgttcttgta cacattcatt tatagcacga
    32401 ttaatgcttc taattggagc gtttacttcg ctagcaagct gttttttggt aagcagattt
    32461 aattcacttc tataatagtg taaggatata cacctcagaa gtcgttcttt aacacttaaa
    32521 gatgataatt cttcaattaa ttcagcattt acaactaatt tactagatat ttctcgaatc
    32581 agaaattttg tagcttcaaa atctcccctt aaccagttta aaaaatcatc tctatgaagt
    32641 ttatatacat tacaatctgt gaaagcgaca atttcaacgg gttttttccc atcattaaat
    32701 tgttccattt ctccaaaaaa actaccactt ctataaagat gaatagttga gatgtttcct
    32761 ttcacattta agatgaatgc ttctgcaata ccgtcaatca taagaattac atattcattt
    32821 tcttgctcag caaaaagaat agtctctttc tttttatacg tttttttaac aagcttcttt
    32881 ttaatatctt cattcataaa atcaattaaa tgcatattca tccctcctta ccaaatcttt
    32941 gattaaatta tatcatcttt caattagtta ggtaagatag taaaaatata tttacataga
    33001 aactattcca ttattatttt ccagttgtca tttttctgca aagtaagctc aaactgagaa
    33061 agctgttgac aatagaacta gtaggaatat atgagcgaac catttcagta ttcaactgtt
    33121 gtaggttttc agtcagataa ttcttcaaac gttcaattcg tttgtcctgt gaatcttgcg
    33181 atgctctaac cagtttcata gatagcgttt taccttgtgc cacctgcaaa aaagaccatg
    33241 cttcgtcaag gtcaactatc ttgaagctag agcggtcagt atgaataaag tctaatgcaa
    33301 aggtactaat aacaatcaac atagcaacac taagaagttc tattgttgta tattcttcaa
    33361 agctagtgtt ggcatctggc aatacgaggt cagcaacttg aataatattc agctgtttct
    33421 ccaagctgat agactgcttc acatttccgt caccaaacag tagatgtgca aaatcatagt
    33481 ctacaaaact ttcgatatgg tcggctatgc tgttactgat agaagtatct tcttttcgta
    33541 attcatcaat gacaagtaat aaccctcgct gttcactttg ggttacattg cggattgctt
    33601 ttctaagcac tgggaactta tcactgtcac gactggagat accagcaagg aatgttagga
    33661 tatcaatcgc taaactttca gaatcatttt tacggctcaa aatgacgtaa gggtcaagca
    33721 atcctttgtt ttcatcatta ctggttaaat tgacaatgtt tatctcatgg gctatttctg
    33781 gtagcgtttc tttccatttg ccgcgttctg ctttagggtc aactatgacc gcctgtccgc
    33841 cgaatagtac cgcatagtaa acaagcatat tgttactgaa taatttacca ccacccaatg
    33901 aaccgataaa cgcagacgct aacacattgg taatagaacc ctttacccat tgactagcaa
    33961 gggcaggttt tagatagaca ttctttccag tgtccagatt gtagccaaga tagatatcct
    34021 catgttcgcc aagggtttgt gtcgctccaa aaccaagtcc agccaaaaaa tccgaagtga
    34081 catattgaat ataatcattg atgtaacgtt tgctgacagg aataaattcg ccatgcaatc
    34141 ccaacatatc cccaaaggga cgcacgagct tgagtgataa gtcgtcgtaa aagtctttga
    34201 cttcatcaca acgccttttt agctcgtcaa gatcctttgt agatacacgg acaacatagc
    34261 taagtttata catagattct ttacagttaa aagtctgttt ttaaaaaaat gtataaatta
    34321 taaatcaaaa tatagatact atcaatgacc cactaggtgc aaaaagcatt gatatggtta
    34381 aattatcaga cgctaaaagc tgggctatcc gaatgagtga agatgggtgt gcttataaat
    34441 ccattgacaa ttataagcgc tcactgaaag cgtcgttttt catggcaatt caagacgact
    34501 gtattagaaa gaatccattt aattttcagt taagtgatgt tttggaagat acaacagaac
    34561 caaaaatcgc tctaacacca gaacaggaaa tgggcttatt atcctttatg gagagcgata
    34621 agacttattg taaatattat gatgatgttg ttatcctctt agaaacagga ctacgcattt
    34681 ctgaattatg cggtttgact ttagaattgg atatgaaaaa taaagccatt atggtagacc
    34741 atcaattgtt gagtgatact aagattggct attatgtaga agttccaaag accacacagg
    34801 gtaagcgaga aattccaatg actgaaagag cttatcaatc atttgaacgt atcgtggaac
    34861 ggcgtaagaa cgctaaagca gaaccaatca ttgttgatgg ttaccagaac ttcctatttc
    34921 ttaaacaaga tggattacca aaacaagcaa ctcattatca aagcatgtta agagcgttag
    34981 tcaagaagta taataagacc catgaggaca agctaccaaa gattacgcca cataccttta
    35041 ggcatacact ctgtacaaat atggctaaca aaggaatgag tcctaatata ttgcaatata
    35101 ttatggggca taaaaacatt actatgacgc ttggctatta tgcacatggt tcatttagtt
    35161 cttcgagagc tgaaatggaa cggttataat cctagtagta tgactagagt ttactactta
    35221 tttactactt ttgatggcat tcttatacgg ttaaatgcca acttatgcaa agtatcttcc
    35281 aaaaagaaaa tgccgatact ccttgtagat acaggatata tcgacattta ccaacttatg
    35341 aaaagataat caaatttaat ataagttttt caaataaaaa gataccaaat tctttttcga
    35401 gaatctatat aacattactt aagcttggtt ggggtataaa caattgagta gctacttttg
    35461 tatggtgttc aactttagtc aaaactctta agtaatgcaa atgatttaga ttcattaata
    35521 ttcaatctct tcataagtag tgaaattcga gttatttctg cataattata ctatgattta
    35581 tagattaatt ctattagcat ccagaataat attgtttata tgagtaaaat ttaaagaata
    35641 ttgtgaaaat tataggatgt taaaataaat ttaggtaaaa aagaagtgtt tgtatattat
    35701 agagtacctt ttctaaaata tgtgggattg ttttaagaat tagaataaaa attattttta
    35761 aattagtatt tttataatgt atagtatttt taatgtataa ttaaattgtg cagaaaatag
    35821 aatttaaagt cctatagaat ttctgattat ggaggagaat atgaaaagaa aagttcaagt
    35881 aaaaaatatt actattggag aaggtagacc taaaatttgt gtaccaatta ttgggaaaaa
    35941 taaaaaagat attataaaag aggctaaaga gttaaaagat gcatgtttgg atattataga
    36001 gtggcgagtt gatttttttg agaatgtaga aaatattaaa gaagttaagg aagttctcta
    36061 tgaattaaga agttatatac atgatatccc tttattattc acatttagaa gtgtagtaga
    36121 agggggagaa aaattaatat caagagatta ttatactact ttgaataaag aaatttctaa
    36181 tacaggttta gtagatttaa tagatgtaga gttatttatg ggagatgagg tcatagatga
    36241 agtagttaat tttgctcata aaaaagaagt gaaagtaatt atatctaatc atgattttaa
    36301 taaaactcca aaaaaagaag agattgtatc tcgtttatgt agaatgcagg aattgggcgc
    36361 agatttaccc aaaatagctg ttatgccgca aaatgaaaaa gatgtgttag tgctacttga
    36421 agctacaaat gaaatgttta aaatttatgc agatagacca ataataacaa tgtcaatgtc
    36481 aggaatgggt gttataagta gattgtgtgg tgaaatattt ggttctgcat tgacttttgg
    36541 agctgcaaaa agtgtttcag caccagggca aatatcattt aaagagttaa attcagtact
    36601 taatctatta cataagagta ttaattaaat taattatata gaaatattag acggtatttt
    36661 tgaagatata aattaaatat aaaaaacttt tcagatagta aaatttaaat tgggcaatta
    36721 tatctgaaaa gcaactattt agtgtgtatt ttgaaagtga tatagaaaat cttataaatc
    36781 aactatggca tatttaaaat atgccattaa aacctactag acattagatt atagatggta
    36841 tactagcatt tctattattt atagatagta agttttcata ataactgttt tatctaaagt
    36901 agaagatata tttgtattat gtatatttgt cattattttt ttgtaattat gtatattaat
    36961 ggttatatat gtaatataat attatcactc tattttttgt atattgtaca ttacaacatt
    37021 tatatctatc atcatcaatt acagtagaat gtatgggata cttattttca cttaaccaat
    37081 atttttgcat attctttgtc caagactttg gaaacttcaa tactttcttg tttattataa
    37141 catcctcatc ttcaaaaata ttaacttctc tttctatttt tatttttctg ttgtatatct
    37201 tattgataga ttctttgtcg agttttatgt ctctatacat atcagggcaa tagcaacaaa
    37261 cataatttct tttttttcga cgtttaaatt tcacaacttt ctctatgcta atttctaatt
    37321 tttttgtacc atcttttata aatttaatat cttctatgtt ctcgcttgaa aaaatttctg
    37381 tcaatcttaa agtatctaca gtttctctaa ttatttgctt tttataagaa gagaatccat
    37441 atccaaaaga aaggtttaat ttattaggga tgtagccatt tttatattta aacatcttaa
    37501 ttttttccaa ttcagcatta tctttaaatt tcaacttttc atacctaaaa gttactttgc
    37561 taaaaagagt atattctaca tcaatatatt cttttgcaat cttaaattta tcgctacctt
    37621 caatcacaat catagcctat ctccaatcgt tttatattaa aataaggcca tagaacatgg
    37681 tctaatagta ctcattagca ctataagaca atgttaacac aaaaagacaa aaaaaggtta
    37741 tttatttcaa ataacctttt tttttatata aaatataaag aattatattt tattcttttt
    37801 tatgatgtct ataatctgtg atatcagatt tagttctctt gaatttaagt cttttaactc
    37861 ttcatttata tcattttcta aatatgtttt tccagtggaa gtaatatctt ttataagaac
    37921 atctgcagaa atatttaata tatttacaaa ttttatgaat gttttaagac ttggattctt
    37981 tttaccactt tcgatttgag ttatatacca aggcgatact ccaaccatat ttgcaaattc
    38041 ttttgaagtt aaatttgctt ttttccgttc ttctttaagt cttttaccta tataattcat
    38101 tattgttact atttccctca cttatatttc tttgtattta atgatgcact ttattaactc
    38161 tagcattgca atttgttctt tttcagttaa tttattaatc atttcataac attctaaagg
    38221 tacagagtta tcaacgcctt ctgttgtaat aatatttgca aataacaatt caagtggtat
    38281 gtctaaagca gtagcaattt tcattataat attgatagat gtatttgtct cagctctttc
    38341 aatctgacca atatatgttg gatgtaaacc tgccttttct gctagaaact cttgactata
    38401 tcccaatttt gttctataga cttttattct atctcctatt atttttccta tatttgccaa
    38461 ttattatccc tccactttag attatattca tagtttaagt tgttttatat agactattta
    38521 tactttttta gtctaaatat agactatata tgtttttata ctgaaataag tgctatttat
    38581 ttttgtatag tctgtatcta caataaacaa atatggataa atcatatttt taattagaga
    38641 aatattttca taat
//
LOCUS       DAEMGG010000026        34845 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEMGG010000026 DAEMGG010000000
VERSION     DAEMGG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34845
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.026"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(174..830)
                     /locus_tag="KPK24_001291"
     CDS             complement(174..830)
                     /locus_tag="KPK24_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2298976.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(885..1514)
                     /locus_tag="KPK24_001292"
     CDS             complement(885..1514)
                     /locus_tag="KPK24_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2298977.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(1645..2286)
                     /locus_tag="KPK24_001293"
     CDS             complement(1645..2286)
                     /locus_tag="KPK24_001293"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG2298978.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(2295..2966)
                     /locus_tag="KPK24_001294"
     CDS             complement(2295..2966)
                     /locus_tag="KPK24_001294"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG2298979.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLSDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(2971..3855)
                     /gene="rsgA"
                     /locus_tag="KPK24_001295"
     CDS             complement(2971..3855)
                     /gene="rsgA"
                     /locus_tag="KPK24_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG2298980.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(3953..4690)
                     /locus_tag="KPK24_001296"
     CDS             complement(3953..4690)
                     /locus_tag="KPK24_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG2298981.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(4798..6813)
                     /gene="pknB"
                     /locus_tag="KPK24_001297"
     CDS             complement(4798..6813)
                     /gene="pknB"
                     /locus_tag="KPK24_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG2298982.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(6830..7582)
                     /locus_tag="KPK24_001298"
     CDS             complement(6830..7582)
                     /locus_tag="KPK24_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG2298983.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(7602..8633)
                     /gene="rlmN"
                     /locus_tag="KPK24_001299"
     CDS             complement(7602..8633)
                     /gene="rlmN"
                     /locus_tag="KPK24_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG2298984.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(8641..9966)
                     /gene="rsmB"
                     /locus_tag="KPK24_001300"
     CDS             complement(8641..9966)
                     /gene="rsmB"
                     /locus_tag="KPK24_001300"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG2298985.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(10028..10729)
                     /locus_tag="KPK24_001301"
     CDS             complement(10028..10729)
                     /locus_tag="KPK24_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG2298986.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(10798..11550)
                     /locus_tag="KPK24_001302"
     CDS             complement(10798..11550)
                     /locus_tag="KPK24_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG2298987.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTVVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            complement(11560..12489)
                     /locus_tag="KPK24_001303"
     CDS             complement(11560..12489)
                     /locus_tag="KPK24_001303"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG2298988.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(12504..12944)
                     /gene="def"
                     /locus_tag="KPK24_001304"
     CDS             complement(12504..12944)
                     /gene="def"
                     /locus_tag="KPK24_001304"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG2298989.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(12962..15487)
                     /gene="priA"
                     /locus_tag="KPK24_001305"
     CDS             complement(12962..15487)
                     /gene="priA"
                     /locus_tag="KPK24_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG2298990.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(15726..16925)
                     /gene="coaBC"
                     /locus_tag="KPK24_001306"
     CDS             complement(15726..16925)
                     /gene="coaBC"
                     /locus_tag="KPK24_001306"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG2298991.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(16930..17196)
                     /locus_tag="KPK24_001307"
     CDS             complement(16930..17196)
                     /locus_tag="KPK24_001307"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG2298992.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(17199..17816)
                     /gene="gmk"
                     /locus_tag="KPK24_001308"
     CDS             complement(17199..17816)
                     /gene="gmk"
                     /locus_tag="KPK24_001308"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG2298993.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(17846..18727)
                     /locus_tag="KPK24_001309"
     CDS             complement(17846..18727)
                     /locus_tag="KPK24_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG2298994.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(18731..19561)
                     /locus_tag="KPK24_001310"
     CDS             complement(18731..19561)
                     /locus_tag="KPK24_001310"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG2298995.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(19848..20882)
                     /gene="ltaE"
                     /locus_tag="KPK24_001311"
     CDS             complement(19848..20882)
                     /gene="ltaE"
                     /locus_tag="KPK24_001311"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG2298996.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            21178..22953
                     /locus_tag="KPK24_001312"
     CDS             21178..22953
                     /locus_tag="KPK24_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG2298997.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(23168..24820)
                     /locus_tag="KPK24_001313"
     CDS             complement(23168..24820)
                     /locus_tag="KPK24_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2298998.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(25119..26330)
                     /locus_tag="KPK24_001314"
     CDS             complement(25119..26330)
                     /locus_tag="KPK24_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG2298999.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(26614..27147)
                     /gene="pyrR"
                     /locus_tag="KPK24_001315"
     CDS             complement(26614..27147)
                     /gene="pyrR"
                     /locus_tag="KPK24_001315"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG2299000.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(27507..28454)
                     /locus_tag="KPK24_001316"
     CDS             complement(27507..28454)
                     /locus_tag="KPK24_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG2299001.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(28423..28869)
                     /gene="lspA"
                     /locus_tag="KPK24_001317"
     CDS             complement(28423..28869)
                     /gene="lspA"
                     /locus_tag="KPK24_001317"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG2299002.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(29179..29958)
                     /locus_tag="KPK24_001318"
     CDS             complement(29179..29958)
                     /locus_tag="KPK24_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2299003.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(30013..30495)
                     /locus_tag="KPK24_001319"
     CDS             complement(30013..30495)
                     /locus_tag="KPK24_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG2299004.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(30761..32182)
                     /locus_tag="KPK24_001320"
     CDS             complement(30761..32182)
                     /locus_tag="KPK24_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG2299005.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(32328..33098)
                     /locus_tag="KPK24_001321"
     CDS             complement(32328..33098)
                     /locus_tag="KPK24_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2299006.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(33103..34305)
                     /locus_tag="KPK24_001322"
     CDS             complement(33103..34305)
                     /locus_tag="KPK24_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2299007.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        11112 a         6377 c         3488 g        13868 t
ORIGIN      
        1 ttaaatatat ttaaaattaa tatacaaaaa aaagtagttc ttattgtatt aattaaatta
       61 atgatttgaa atataaaatt aatatcaata aaacctactt tttaattttt taaatattta
      121 tttttaattt attttgtttt taatttactt cgattttaaa atgtccttga cttttatttt
      181 ataaatgtac atatttcttc tactggtttt ttagatggac tcttcaaatt gtcctctggc
      241 actccaagag ctaacattgc acctaaaaaa tactctccct tttcaaaacc agtttctgct
      301 tctaaaacag cttctatctc atctgctgca tagttttgac ttgtcaacca acaagaacca
      361 tatccaagtt caattgctga tagtgtaaag ttttcaattg cagcacctag actctgcata
      421 cctggatttc ttataaataa tttatctata gtctccttag gtgcatctat aagctctaat
      481 tcataatatc ctgatggatt atataccttt gtaaaaacta atactaaaac tggagctttt
      541 aagtaaaata gtgtaaaatt tttaacaaat tttctaaatc tattagcctt atccactgat
      601 actttatcca tttctacaag tatttcttgt tgctttttag ttatcacatc tgctatcttt
      661 tccattaaat cacggcgttt tataacaaca aaatgccagt tttgaatatt cttgcctgat
      721 ggtgctgcac ctgctgcttt tatcattttt aaaatatctt catctgatac atcttgattt
      781 ttaaactttc ttacactttg tcttttaaaa atagtatctt gtaattccat ttttaaatcc
      841 ccccataact tttttattat tttaatataa tttttaaact catcttattt taatatatca
      901 tgttctaaca aatctttaaa tgaatctata aaataatcat atacatctac attcccaaac
      961 ccatatttgg catatataaa aggtatacct acaaacttag cactatttgc atcaccttgc
     1021 gtatcaccaa catagattgg atttattaat ttatttcttt caattataag tttattattt
     1081 tctcctttat gcaaaccagt attaccagga cattcaaaat ctataaaata ctgttctagt
     1141 ttatgtgctt ttaaaaaaga ttctatatat ccagcttgac aattacttac tataaataat
     1201 ttatattttt tagatagtaa tttaagagta tcttctatat catcaaataa tttagctcca
     1261 ttttcagata aatagtcaca ttctttcaaa gaacattcat tcaataaatt cattctttct
     1321 tcttcactta aatttggaaa taaacgattt gctatttcct caaaagaaag ccccataaga
     1381 cttctaaata aatcttttgt gacacttaat tctatttcct tatgctcact taaaacatcc
     1441 tgccagactt tgcatacttc ttcagttgaa tcccacaatg ttccatctaa atcaaaaata
     1501 atactatcta ccacaaaaaa tcccccttat gtcaaagttt attatatatt tataattata
     1561 tactaaaaag cagatacaat cataaatata ttttacttga ttgtatctgc tctacaatat
     1621 aaaaccaatt gtcaaaatca aattttataa atttctaact acaagtacac agccttgctc
     1681 tactttaatc ttacaagaat tactcaacat aacatttgat attccaatag gtacagagta
     1741 tttcatataa taattatcga gagggtattc taagccagta agtgttacac ccttagcatc
     1801 accatttata gctattacag aaatagtgtc ccctgggttt ccatacaatg aaatttcctc
     1861 attttcaact atatacatct cttctttatc agaaagtatc ctagaatata taccatcttc
     1921 ttttaaataa tataatagct ttatatttgc aagagtgtgg tcaatccttc cccctaaagc
     1981 accaaaaaaa tcaatatgat ttgctttaag tgtcttagct aaaaaaagac aaagctctgt
     2041 atcagtctca tcttttttag atggaaattt ttcaaattta acacctttat ttttatagaa
     2101 gtttatcttt tcttcttcta ctgaatctaa atctcctaat atataatctg gcattatttc
     2161 cattttataa gtgtgatttg cccctccatc tgcacatatt atgcagtcat aacattcctt
     2221 aattataata tctcttgtaa cttcgtaatc ttcaatttcg ccatttaaaa caatacagat
     2281 tttcattatt aaatttaaac taattcttca agagaagcat tctttctaaa taaattaact
     2341 gtttcttgaa tgtcatcact tccaaatatt gcagaacctg ctactattac attagctcct
     2401 gcttgtacaa cttcggctac gtttttaggg cttataccac catcaacttc tatatcaatg
     2461 tttaatcctt gactatctat aatagctttt aactctttaa tcttatctaa aacacaagga
     2521 ataaatgatt gtcctccaaa tccagggtta acagacatta ataataccat atctaaatca
     2581 gatagtacat gtttaatcgt atctactggt gtagctggat taagtactac accagctttt
     2641 attccatgtg attttatatt ttgtatagtt ctatgtagat gaacacaagc ctcctggtga
     2701 acagttatta tatcacatcc tgcttttaca aattcttcaa tatactggtc tggattttct
     2761 atcataagat gagcatcaaa taccatattt atattttctt tctttaaact tttgactaca
     2821 agtggtccta atgtaatgtt tggtacaaag tgtccatcca ttacatcgat atgaagatat
     2881 tcacatcctg cactttctac ttttcttaca tcttctaata attttgcaaa atctgctgat
     2941 aatattgatg gtgctaattt aatcatgttt ctaatacctc ctactattat tttgtcttat
     3001 ttcatgtagc agctgtatat aactatcata tctttcttta gatatttctc catttgtaac
     3061 agcttctttt atagcacaat taggttcatt ttcatgaata cactttgaac caaacttaca
     3121 atcattaaat ttatcaaatt ctataaaata atctttaagt tcaacttcct ctatatcttc
     3181 aagtgcaagg gaactaaaac ctggtgtatc tgcaaccatt ccaccaaatt ctaattttaa
     3241 aagttcagcg tgacgagtag tatgttttcc cctttttatt ttatcactta caactcctgt
     3301 ttgaagttta aaattttcat ctatttcatt taataagctt gattttccaa ctccagatgg
     3361 tcctgcaaac actactacat tttcttttaa ttcttctttt actttatcta tatttaactt
     3421 agtaatattg cttacaggaa tgactttata accactaagt tcatatatat ttttaacagt
     3481 ttctaatgta tcattatcat ctaaatctgc ttttgtaagg attattacag tttctaaatt
     3541 ttctttttct gccaaaacta taaatctatc taaaagagat aagtttggtt taggattttt
     3601 tattgcgaat actattaatg ctttatctac atttgcaata ggaggtctta taagttctgt
     3661 atctctactg tcgatttctt ctaagatacc ctttttttca tcttcatcaa caatgcttat
     3721 tttgactcta tcaccaacca aaggtgttat tttctgcttt ctaaatatcc ctctagcctt
     3781 acattcataa ataccattat aagtatctac ataataaaat ccactaatcc cctttataat
     3841 ttttccttct agcatcaaat gcctcccttt ataaaattta tataaacaca tactgtttat
     3901 gagtagtata tcgtgttatt ctttcctatt tttatataat accacatttt ttttaaataa
     3961 caaatcctcc gtttggacta attacctgac cagtgataaa atttgatttg tctgatgcca
     4021 agaaaatagc acaatttgct atatcttctg gagttcctag cctcattaga ggggtttctt
     4081 ccacaagagc gtctatatct tcctcattat atccagataa catgtcagta ttaatgacac
     4141 ctggagctat gctatttact ctaatatttg atggtcctac ttctttagct aatgcctttg
     4201 tcatacctat tatacctgct tttgttatag aataatgcac ttcacaagac gcacctgata
     4261 taccccagat tgaagatata ttaattatat ttcctttttt ttctgatatc atgtatttta
     4321 aagcaacttg tgaacaataa aagcttcctt ttaaattaat attcatcata ttatcccaat
     4381 cttcatcagt tatatctgta aacaacttat cttgacttac accagcattg tttacaagta
     4441 catccaatcc tccaaattct tttatacaat aatctaccat atcctctaca tcttctctat
     4501 ttgaaatatt agccttaaaa agttttacag aaaaattttt ttcatttaat atagtgtaca
     4561 actctttagc ttcattttct gacttattaa aatttactaa tacattgtaa ccttcttttg
     4621 caaaagcttt agacatagct ttaccaatcc ctctagctcc tcctgtaatt aaaactgttt
     4681 tcttcttcat aaatactttt ctccttatat tataaccaat tcagtatttt atacaaaaca
     4741 aactgccctt taaagaaaat attttctata aaaggacagc tcttatttta ttaatattta
     4801 ttctgtttta ccaatgtttg cgccagttcc tgatgaagtt cctccattat ctcctgagtt
     4861 tgagcctcct gaactatctc cattgtctcc tgagtttgaa cctcctgaac ttcctccatt
     4921 gtctcctgag tttgagcctc ctgaacttcc tccattatct cctgagtttg agcctcctga
     4981 gtttccacca ctatttcctg agtttgaacc tcctgagtta cttccattat tgcctgagtt
     5041 tccaccacta tttcctgaat ttgagcctcc tgaatttcca ccactgtttc ctgagtttga
     5101 accaggttct gttggtgtat tattctctgg tgttgttggt tttttatctg gtactgttgg
     5161 agtctgtgta tttgattttt ctgaaccttt gcttacatat agatttacct catcaccttc
     5221 ctgagctctt gatgagccag cacctggact ctgacttaaa actgtacctt ctttatatat
     5281 tgagctgtat tcatacttaa cagttcccac ttttaagtta ttttcttcaa taagttgttt
     5341 tgcattactc aaagtaagtc ccactacatt aggaacagat gcctgacttg aacctttact
     5401 tacgacaaca tttatagtat cacccttttt gacatttgta cttccacctt caggtgtctg
     5461 tgatattata caattttcat caacttcact attatactct tcacttttta cactcaggta
     5521 taatccttct ttttcaacag cactttgagc ttcttctaat gtcatattta caagattagg
     5581 aacagtcaaa gatttattgc caaaccctcc agcaaataaa aacttatatg ctaagaatac
     5641 ttgagcacac aatatcagta tcaaaacagc cgcaattact tttaatcttc ttctagattt
     5701 tgggctttcc tgttttttct tctttttctt agttctctta cttggagtac tcttaggtct
     5761 ctgattcttt ttagctctca ttatctcttc ctcttcttca tcttcatcat catcttcttc
     5821 ataaaaatca tcatagtaat cttcatcatc atccaaatca gctacttcaa caggcttaac
     5881 cactttttca ggagctggtt ttgcaagtgt tggatttaca actttattaa tttctttttc
     5941 atcaattttc ttagttgcaa aatcatcata ttcttttata aaatctaaat caatattttt
     6001 ttctatatac tctatatctt ctatcaattc ttcagcagtt tgatatctat ctgcactaga
     6061 tttttctgta agctttttta ttgtagttct tacactttga ggaatcctaa ctttttcttc
     6121 tgatgtaaag tctatatcat cattaatgtg ttgaagtgct atagaaattg gactatctcc
     6181 cctaaatgga acttttccta tcaacatttc atataaaact atacctagag aatacaggtc
     6241 agcattatta gttacaaatt tacctttagc ttgctctggt gaaaaataat gcactgaacc
     6301 tattatacta ccaatattag tcattgttga atttgaaaca gctttggcta taccaaaatc
     6361 agccactttt actactcttc cttcatttga tatgagaata ttatgaggtt ttatatctct
     6421 atgtataatc ccctttttgt gcgctgcact aagtgccata gctatttgtt ttgttatatc
     6481 taatgcagta tattcatcta atataccttc atcttgtata atctctttta gattcttccc
     6541 atctacaaac tccattacaa tgtagtgaac ttttccatct tcaccaacat cataaacatt
     6601 tactatattt ggatgtgaaa gacttgctac tgcttctgcc tctcttttaa actttgttag
     6661 aaattcatca tcatctacaa attcaggtct aagtacttta agtgcaacag tcctgtttaa
     6721 taatctgtct ttagcctcat atacaaatgc cattcctcca tcaccaatct tcctgatgat
     6781 ttcatatcga tttcctaaaa ttgtatctcc cacaatatca ccgtcctaat tatactttta
     6841 tcactataac cgaaacatta tctttaccag aaacatcatt ggccatactt atcaattctt
     6901 cacttatatt actagttctc tcatattcat aatctaatat taaatctctt atatcttcat
     6961 catcaataaa cccagttaat ccatctgtcg caagaagtat tatatcactt tttttgattt
     7021 ctttcttaaa aatatctaca acaaccatat catctgttcc aattgctcta gttattctat
     7081 tcctctgtgg atgtcttctt gcctcttctt ctgtaatgac atgggccatc ataagttctt
     7141 caaccacgga atggtctata gttattttat caaatttttc atctgttaac aaataacatc
     7201 tgctatctcc aacatttgcc acatataaga cattattata cactatagcc gtaactaatg
     7261 tagttcccat tccttcaaat tctatatctt ccatagattt tttgtgcaca attgagttta
     7321 cattattata agcctgtttt aatatatcat ctatatagtc tatttttaca ttatcatgtt
     7381 gtaataagtt ttcctttaga aaatcaatta tattttctac tgctaattta ctggcgactt
     7441 cacctttttt atgtccaccc attccatcag ctatggcaaa tattcctatt ggaccatgtt
     7501 ctgtatctat aacttctccc tcacaatagt cttcattgtt ttttcttatt tttcctatat
     7561 gggaggcaca actataaacc atacgacttc ctccttacaa attacttgta ttttcttctc
     7621 aattgcccac aagctccact aatatcagaa cccatagaca tcctaactgt ggcaggtata
     7681 ttgttcttct ccaaactatc cctaaattta taaataaatg ctttatctgg cttttcatat
     7741 tctctttctt caactttatt tattggaatt aaatttacat gacatagcat accttttagg
     7801 agcttagcta atgcctttgc ttctttttct gagtcattta ccccttttat aagagaatat
     7861 tcaaatgtaa cccttctatt tgtctttttt atataatatc tacatgcatt caatatttct
     7921 tcaatagaat aagcatttgc tacaggcata atttttcttc gttcttcatc atatggtgaa
     7981 tgaagtgata aagctaaatt tatagctatt tctaagtctg ccaactcata cattttaggt
     8041 actattccac aagttgaaag agttatatgc ctatacccta tattaagacc atttttttcg
     8101 ttaattattt ttaaaaattg ttttgtattt tcaaagttat caagtggctc accactcccc
     8161 atcaatacaa gatttgatac cctcttacca gtatcttctt gaattttaat aacttggtct
     8221 aaaatttccc atggctctaa gtttctaatt aatccatcca ttgtagatgc acaaaaatta
     8281 catcccatcc tacaacctac ttggttagaa acacagacag taactctact atcataatcc
     8341 atcatcacag tttctattat atttccatca tctaataaaa acaaatattt ttttgtatta
     8401 tctaccttag attctaactt taattctata tctatatgcc ctatacatga aacctcttct
     8461 aatttatttc tcaaactttt tggtatattg ttcatatcat caaaagtttt tgcaccctta
     8521 tatatccaag agaatatttg acttcctcta aagggctttt catctatagt tttcataaat
     8581 tctttaagtt catcttcagt aaaatttttt aatactatct ttttcttttc cattctctca
     8641 ctaccttatt ctctttaatt ttgctatgaa aaaaccatct ataccatgaa tattaggata
     8701 tatctttaaa tatcctttat cttgattgtc taagtctaca tttactgtat caattggaac
     8761 aaattcaaag ttgtgatttt cttcaacaaa agaagtaact atatttatat tctccacatc
     8821 ttgaattgta catgtactat atactaaagt tccaccaact ttgacatatt tagatgcatt
     8881 ttctaaaatt ttcttttgta tagaagtgat gtcttttaac tcttcttctt tcttatactt
     8941 aatttctggt ttacgtctta ttataccaaa accagagcaa ggaacatctg ctaaaacata
     9001 atcaaattta cttatactat tttcatcaat ctcagaagca tcaaatcttt ctacaaacac
     9061 atttttaagc cccaatctat ttacagtaga gtttataagc tttagtttat gctcaaaaat
     9121 atctctagct actacttgac cagtattgtt cattaaagta gctaagtgag tagatttccc
     9181 tccaggagca ctacatacat ccaaaacaaa tgagccttct tttggattaa ttactttccc
     9241 tactatcata gaacttatat cttgaattgt aaataaacca gccttaaata attcattttt
     9301 ttctatattt ttcatttttt cgactttaat agcttcttct accataggaa cttttaaaca
     9361 tacaataccc atttcactta atttttcaat aagttcttct ctacttattt taagtgtatt
     9421 cgttcttata tagatactag gcttttcatt atttgcttcc aacaaatctt cagcaaattc
     9481 ttgtccaaat ttatctatcc agttttttat aatccaagag ttataggaat actttataga
     9541 taagtatttt acactatctt cctcagttat gtccattata gtctctttag ctcttatttc
     9601 atttctaaga atagcattta tgaaaccaga agatttttta tcatatttct tagataattt
     9661 tacagtttca ttaactgctg cataatctga tatactatct aaaaataaaa tctgatatgt
     9721 tcccattctt aaaaagattt taacataagt tgacattttt tttaccttta tttttgaaag
     9781 tttattgatt atataatcta aataatattt attttctatt actccataaa ttaactctgt
     9841 tgcaagtcct ctatccatat cacttatttc taaattttta aaatgcttat ttattgcaat
     9901 atttgaataa ttgtcatttt tttctatatc acataatact ttaaatccaa cttctcttgc
     9961 atccatataa atttctccct tttattaaac ataaaaaagg gttgtttcta aacaacccac
    10021 tttactttta gtcatttctt ctttgagcta ttagtaacaa tctaagtaat tgaagtattg
    10081 aaaccaatgc tgctgcaaca tatgttaaag ctgctgctga aagtactctt ctactttgtc
    10141 ttgactcact ttcatctata atacctaaat tcccaagttg aacgattgct ctacttgaag
    10201 cattaaattc tacaggcaat gttattattt ggaataacac tgaagccgaa aacaatagta
    10261 taccaatttt taaaaatgga ccagccataa taaatcctaa aaatattaaa aaccaagata
    10321 tacttgaagc aaaattcact actggtacta aactacttct tatttgaaga ggtgcataac
    10381 ctttagcatg ttgtatggca tgaccacatt catgtgcagc tactgcaaca gaagttatag
    10441 atgttccata atatacatct tctgacaacc ttacagcctt tcttcttggg tcataatggt
    10501 cacttagatg ccctctaacc atttctatct ttacatcata caaaccattt gaatcaagta
    10561 ctcttcttgc tgtttgctct ccagtatatc ctcgacgtgt atttactctt aaatacttat
    10621 ttgttgttga actcacttta aattgtgcat atatagtaag taatatagct ggaattaata
    10681 caaccattgt tgggtcaaat ccccaaaaac cgccatatac agggtacata aataacactc
    10741 tcctaaaata tatttttatt atacatcctc ttaatattca cttaacttaa aaacagttta
    10801 attacaaact aatatatttt ttcctgttaa aaagcctata gctttttcaa tatccatcat
    10861 gtctacatta gtatttacac aaggtccatt aggtcttttg ttaaatacac ctaaaacagg
    10921 aattttctta atatcttgta ttccagatgt caaatctctt tcacatgcaa cagctacaac
    10981 agctttaggt ttagtgtcca ttataatttt tctagctaga gttcctcctg tagctacaaa
    11041 tatttttaca ttagtttttt cctttagctt aattaattct cctatgttgc atcttccaca
    11101 ttctttacaa ttatctatat cattagtaac ttttaattta caactattct cttgaataca
    11161 atgaggtatt aaaattatta tatcctctgg attaaaatta tatttattac tgtatatata
    11221 ttcattattt aactttatat atattcttct aatatcactt tttgacatac caaatgatga
    11281 tgcaatgaaa gatataatag gaaataatcc actgacaata ttaaaattta ttttcattat
    11341 atttgagtta acatactttc ctttaattac tctataagta actactgttg aacatagtat
    11401 tataagtatt agtgatatta ttataatatt tatcatcaag gagaatattt gagttaaact
    11461 tattataaaa aaattaacaa ctcctatcaa aattcctaat attaaaatca aaatcccaac
    11521 acttgttaaa tacttcttta tatctgtcat acttttcacc taacccaata ctaaaccagt
    11581 ttctatagta ttccctttta tatattcacc aaccaacatt ctctttttat tcggcatctg
    11641 aatttcttca attaaaagta cattatctat agtacttact tttatcccat ctttgcttac
    11701 atcaattatt gtacctacat cttttgtgct tttttcatct aacactttag ttttccaaac
    11761 tttcattatg acatcattat aggtagtata agcacttggc catggattta ctcctctaac
    11821 taaattgtgt atttctctgg cactttttga aaaatctatg ttacctaatg atttattcat
    11881 tattggcgca taggaaaatt cttcatgatt ttgtacttct cttggagcat ttccttcttc
    11941 aattaatctt aatgtttctt ttaaagtttc tgctcctata ttcatcattt tatcatgaag
    12001 ttctcctgct gttatatttt catcaagatt tacttctgtc tttagtatca tatctccagt
    12061 atctaatcct tcatccatat acatagttgt aacaccagtc ttttcttcac catttattat
    12121 tacccaattt ataggtgccg cacctctata ttttggaagt aaagaaacat gaacatttat
    12181 acatccaagt ttaggaatct ctaatatttc ttttggaagt atctgtccaa aagctacaac
    12241 tactattaca tctggattta aagatttaat tttatctata aattctttat ctctagcctt
    12301 tactggttga tatacaggta tattattttc aattgccagt tcttttactg gactcatacc
    12361 tagtttttta cctcttcctt ttggtttatc tggttgagtt acaacaccca atatttcata
    12421 cttctcatct attatttttt gtaaacatgg aactgctata tcaggagttc ccataaatac
    12481 tattttcatc aaatcaactc ctactacttt tctattttgt caacaaataa aattccatct
    12541 aagtggtcta tctcatgaca aaatgctcta gctaaaagtt cttctccctc taactctatt
    12601 gtttcaccat ttctatttaa agctctgacc tttacataat ttggtcttct aacatttccc
    12661 gcttctccaa caacacttaa acatccttcc tcatctattt gctccccaga tgtttctatt
    12721 atttcaggat ttataagttc tataagctct tctccaatat ctattactac tactcttttt
    12781 aatattccaa cttgaggtgc agccagtcca acaccatctg catcatacat agtttctgcc
    12841 atatcatcta ataattgtat tatcttttca tcaatttttt caaccttttt tgattttttt
    12901 cttaatactg gttctcctat ttgaactatt tgtcttaaag ccattataat tcctcctaaa
    12961 tttataatac actgtttgga ttaatatcta tagatacatt gatattctta tcaaatatca
    13021 aatctcgttt tgttatacat atatatttta ttataccctt taagagatta atttcaatat
    13081 tttcatcttt aaagagtatt tgccatctat aattttgatt tatttttgat attgaacatg
    13141 gatttggacc taatataaat cctaaatcat ttatacccct tttttctaat aaataaatca
    13201 atgatgcatg catatttttt atatttttta tcactaatct ttcatcttca ccactaacaa
    13261 ctacacttaa catattatta aaaggagaat atccaaatgc ttttcttatt tttatctcat
    13321 cttcataaaa accctcataa tcatattcaa tagcatgctt aatagcatag tgttcagtgt
    13381 cataagtctg aagtactact tttccctctt tatcagctct ccctgctctt ccagagactt
    13441 gagttatcaa ttgaaatgta gtttcaaaac ttttaaagtc tggaaaattt aatatcatgt
    13501 ctgcagacaa aatacctact aaagttacat tctcaaaatc cagcccttta cttaacatct
    13561 gtgttccaat aagtacatct gcttctttat ctttaaattt atttagtatc tcatctaaag
    13621 cacctttttt tgaagttgta tctttatcca ttctcaaagt ttttatatct ggaaaaatat
    13681 attttaattc ctcttcaatt ttttgagttc caactccaaa tggcttaaca taagtacttt
    13741 tacattcagg acactctttt ggaatttctt tctcatatcc acaatagtgg catcttccag
    13801 tattgctttt cttatgataa gttaaagata tatcacaatt ttcacattga aacacatatc
    13861 cacactttct acaagataca aaatttgcat aacctcttct gtttaaaaat agtattactt
    13921 gattattttc ttcaatcgca taacgtattt ctttttgtaa tttcaaacta aatatacttc
    13981 tatttccttt atctaactca tctttcatgt ctacaacttc aatgtttgga agaggcttat
    14041 catttgctct actttttata tttataagtt tgtattctcc agattttgcc ctataatact
    14101 cttcaattga gggcgttgct gaacctaaaa taagagttat attatttttt aatgccataa
    14161 atctagcaac ttctatagca ttaaattttg gattcttctc agacttatat gatgactcat
    14221 gacattcatc aattattatt aagcctaagg aattaaaagg tgcaaataaa gctgacctag
    14281 ctccaattaa cactcttact ttacctgctt tgacagcttt gtaaacgtca tgtttttctc
    14341 cttcagatag tttactgtgg aacactccaa ctaaatctcc aaacctactc tttagcctat
    14401 ctattgtttg aggagttaat gctatctcag gtactaagaa tatactgtct aagccttgat
    14461 ttagagcaaa ttctattatt tccatgtaaa cttcagtttt tccacttcca gttactccat
    14521 gaattaagta tggtttttta tcatctacaa acatattcga ctttatctca tcaactgctt
    14581 cttgttgctc attgtttaat tttatacttt ttagtacgga tttgtaaata ctttttggct
    14641 ctctataata atccttaaat tctagggtta ttagcttttt cttacttaga gaatttatac
    14701 tttgttttga agcatttaat aaatccaata aatcatttat ctctacattt tcattatttt
    14761 taagaaaact taatatctct ttttgtttac ttcccacatt tattttattt tgctctatat
    14821 aatcgtcaat cttatcactt tctaaactca aagatatata acatactttc ttttcatttt
    14881 tgtgattttt atattcccaa cataatttta taattccatt tttattcatt gtgtacaaag
    14941 aactatttaa gtttggtata tattttaact tatcaacctt aattttacct ttactatcaa
    15001 tcactttctc tactatttct ctatttttat cacttaaagt tgatattttt tccttcaatt
    15061 caatatcatt taatccactt aaactttcac ctaataggac tactttataa ttatttaatt
    15121 tgtatccctt tggatatatc aaatttatac attctatata agtacacaaa tatctatttt
    15181 tcatccaata aaccaactct aaatcttctt ttctaaatat cggattttca tctaatatat
    15241 caataatttc ttttattttt attttttcat ctaagctatc tgtaaattga aatacaaagg
    15301 cttctgttgg tttattacct tttccaaatg gtactaatat tctgtgccca atttttatta
    15361 catcagataa aaatacagga atttgatatg taaataaatt atctgtatat atagtattac
    15421 ttctaactat tacctttgca tattttttca ttataaaacc tctacttaaa tttaatatat
    15481 tgttcatatt ttaatatact ttacaagata ttaaaatctc agaatttata agcaatttca
    15541 agttattaat attaacttta ttttaataat taactattaa ttatatttta tactttatga
    15601 caaaataaat gctgaaaact acaatttaac tgacatttgc gagtttttac tcgctaaaat
    15661 taaattgcag ctttcagcca ctttagctgt gataaaatac acaaatatca tatttaaaaa
    15721 aggtcttatt tctcaagcaa cgctttaact ttatctaaaa taacattggc tacttcttct
    15781 tttttcatct taggatactc agtaatattt ccctctacat ctattatttt tacaatatta
    15841 gtatcagctc taaaaccagc accttcttta gttaaatcat ttgccacaat aaaatctaaa
    15901 tttttctttt ttatttttcc tttggcatgt tctataaggt cattagtttc agctgcaaat
    15961 ccaattagta tcttatcttc ttttattttt cctatttcat aagcaatatc ataatttcta
    16021 cttagttcta ttgataaatc atcattcttc tttttaattt ttttgtcaga ataagttttt
    16081 ggtttataat ctgcaactgc tgcactttga attactattt tattttcact taaatttgat
    16141 actactgctt catacaaatc ttgtgctgac tctatttgaa caaatttttt aagattttgt
    16201 ggaatagaaa gatttgtagg acctgatatt aatgttacat cagcacctct agctatagct
    16261 tctcttgcta tagaatatcc cattttacca gaagacctat tagttaaata tcttactggg
    16321 tctatactct ctacagttgg accagctgta actatcatac tttgaccttt taaatcttgc
    16381 tctttagtaa gattagtcaa aacttcctct actattatat ctggattagc tagtttacct
    16441 gttccaatat ctccacatgc aagccttcca ctttccggtt caataaattt atatccaaag
    16501 ctctttaaag tttgtatatt tttttgaaca attggatttt catacatatt agtattcata
    16561 gctggtgcta taagaacctg tgcttttgtt gccataacag tagttgtcaa catatcgtca
    16621 gctataccat ttgcaatctt gcctataaca tttgcacttg ctggcactat taagaataaa
    16681 tctgcttttt ttgctaatga tatatgttca acttcccaag tttttggttc ttcaaacata
    16741 tcttctacta cataattttg actcagcgat tgaaatgtaa gtggtttaac aaattctgtt
    16801 gctgattttg tcattataac atggatattt gcatttagtt tttttaactt acttacaaca
    16861 tcacaagcct tatatgcagc tataccgcca cttatgccaa ttacaacagt tttatctttt
    16921 agcatgacct tactcctctt ctgaaatttg ttggtgttgc tcagcatgat gtctagcttc
    16981 ttctatctct atttcctctt ctgtcaataa tctatatgtt atttttccac ttgcaacttc
    17041 tttagttgct acacatacag gcttttcctt agttttattt gaaacatatg gttcttctcc
    17101 atcaattaat tttcttgctc tctttgaaac agtccccact aaatagtatc tgttgtctat
    17161 tttttctagt acctcattga tagatggttt taacataatt ataattcctc cttaaatttc
    17221 tctattataa tatttttata tctgctaact ttattctttt cagatgatat tataccttct
    17281 atttctttag cagatgtttt tacatcttca ttaaatataa aataatcata atccttaatc
    17341 attttaattt cttcatatgc acagctaaat ctcttctcta tttcagcttc tgtttctgta
    17401 cctcttccaa caattctatt ttttaattct tcaagtgatg gtggtagtac aaatataaat
    17461 actccctctg ggcaaacttc ttttatttgt tttgctcctt gcatttcaat ctccagtaat
    17521 acatcttgtc ctttttctaa acattccatt attgccgctt ttggagttcc ataaaaatta
    17581 tcataaattt gagcatactc caaaaactca cctttttcta tcatctcttc aaacttttcc
    17641 ttacttatga agaaataatt tactccatca acttctccag ttctgggttt tcttgtagta
    17701 gctgatacag aaagttttat tgtatcattt tctttaagta gttctttgca aatagtacct
    17761 tttcctgcac ctgatggtcc tgatacaact agcaataatc cttttttcac aaacatttaa
    17821 gcattcccct tctaatattg tattcttact ctatattttg tatttgttct cttatttttt
    17881 ccaattcgct ttttacttct actactaaat tagttatatt tatatcagat gacttcgaac
    17941 ctatagtgtt agtttctcta ttcatctctt gtattaagaa atctattttt ctacctatag
    18001 agtcgtcttt aaatatagta tttttaagtt gccctatatg acttttaaat ctaactattt
    18061 cttctgttat actgctttta tctgcatata ttgcaacttc ttgtgctaat ctattctcat
    18121 ctattatact tgggtcatcc agtaattcac ttattctcaa attcaatttt tctctataat
    18181 cttctacaac actacttgaa tatttttcaa tctcttctat atgatttttt aataaatcac
    18241 atctcatggc aatatcttca gctaattttt taccttcttc acttctcatt tctcttaatt
    18301 tgattaatgc atcttcaact gcttgattca acattgacca caataaatct tcatcttctt
    18361 ccttttcttc tattttcaca atatcaggaa actttgcaac attcataacg ctaatatcat
    18421 ctataatatc aaatttattt tttatttctt ttagtatgtc tatatactga gatgctaatc
    18481 cctcatcaaa atttaagttt acatcttctt tgcctaataa gtcaaacttt atgtataaat
    18541 ctactcttcc tctttttaca taatttttta ctaaatttct aactttatcc tctaaaaatg
    18601 aaatttttct tggaagacgt atgtttatat ctgaatactt atgatttatc gttttacact
    18661 ctactaaaaa gtaataatta tcatccttgt attctcctct tccaaaacca gtcatactta
    18721 tagccattaa ttaaacctcc aaaatacctt cacatatttt tactgctgga cctgtcatat
    18781 aagagaaatc tcctacatct attttaactg ttccaccttg agacgttaca tttacactac
    18841 tttctaccat attaagtaaa ttgcacacta ttgctgatgc agtcatacca gtaccacaac
    18901 ctaaagtata tccacaacct ctttcccaag tataaacatg tatattatac ttatcttcta
    18961 cctttacaaa atttacattc gttttttcag gaaatatttt attattttca atctcttttc
    19021 catatttaca aacatcatct acatctaatt catctactat aactactgta tgaggaaccc
    19081 ccatcaacat agaagtaact ttaagctccc tatctaaaac ctttatactt tcttgcacaa
    19141 aacgttcttt atttgtaagt acaggaacgt ccttagctat aaaacttcct tttcccatat
    19201 tgactcttat agagtttatt tctctattag taagatttac ttttatatct tttataccat
    19261 ctaatgtatc tactgtaaaa ctttcttttt taactataca attatcataa acaaatttac
    19321 aaaaacatct aaggccattt ccacacatag ctgctcttga accatcagaa ttataatata
    19381 ccatttctac atctgcaact tttgaatctt tcacaactaa caatccatcc gctcctacag
    19441 aaaatcttct atggcatact ttttttgcta aagcagtata atcagatgga tttatctggt
    19501 cgaatcttcc atctattgct ataaaatcat ttcctattcc atgtagtttc caaaatttca
    19561 taagtatcgc tccttcgtaa attaacgctt aaactattat aacataaaaa aattcccata
    19621 aacttataat ttatgggaaa agttttgtaa tgacaactat aatttgagga tgtgagggag
    19681 agttgccatt acaattaggg taattatatt atgtttttaa gctagttctt tctatgttta
    19741 tatattaata tttctatatg aattttatgt atgttttatt taaactgtct atgaattttt
    19801 taattaaaca tctggtaact agctataatc cttctagtag atatctatta acatacacct
    19861 attaattctt taagtgtttt taaaacatag tctaaatctt ccttagtaat atctacatga
    19921 gttacaaatc tatattcacc attttccatt ttattaattt ttatatttct ttcaaaaaat
    19981 tcttttatta aaacatcctc ttttataata tcttcactca tcttaaaaaa taccatactt
    20041 atatctggat taacattgtt tacttcaatg ccatcaattt tatttaattc acaagctaaa
    20101 tattttgcat tttcatggtc ttcattcaat cttaaagtca ttttttcaat ggcaattata
    20161 ccagctgctg caactatacc tacttgtctc attcccccac ccataacttt ttgctttctt
    20221 ctagcttttg ctataaattc tttatttcca gcaagtatag aacccattgg tgcagctaaa
    20281 ccttttgaca cacagaacat aacactatca caacactttg ctatttcttt agcttctaca
    20341 cccaaagtta atgctgcgtt aaataatctt gctccatcta aatgtactgg taagttatgt
    20401 ttctcagcta tttttttaat ttctcttaaa ttatcaagac tcacaactgt tccacctgaa
    20461 tatgcatttt ctacacatat aagccctgtc tgtggttcat gtatgtcatc tcttctaatt
    20521 gccttttcaa caagttcagg atttagagct ccactttcgt cttcatctat agttctaagc
    20581 tgaactccag ctattacagc aggtgcccca acctcatgag tcacaatgtg ataaccttta
    20641 ccaataatta tttcttgccc tcttaggcaa tgagtaaata gagctaattg attcccaaaa
    20701 gttccacttg gaaggaaaat agcatcttct tttccaactt tagctgctgc taattcttca
    20761 agtttattta cagtagggtc atctctaaag attgaatctc caacaggagc ctttgccata
    20821 gcttctctca tttcgtcagt aggcatagta acagtatcac ttcttaaatc gataaatctc
    20881 attttttcaa ccttctttca tatttatata ctatagaacc ttaaaattga ttttttctgt
    20941 aaagttcata tactttcata gcttatactc tacacaataa ttatactata tttactttat
    21001 atttaatcta ctttaaatta ttaattttaa aaataaagtt tcctctctaa taaatataaa
    21061 taattataca atttaaatat taatctctat tgtttaaatt ccccctagtc acaaagcgta
    21121 tattatgtgt ataattaatt tactgactca ttttggtcaa agaaaggggt gctataaatg
    21181 gcattagatg gattagttat acattcaata gtcgatgaat tatcatcaaa acttacaggt
    21241 ggaaaaatag ataagataca tcaacctgaa gatgatgagg taatttttaa tataagaaat
    21301 aataaagaaa attttagatt ggttttaagt gcaagtgcct caaatcctag agtttatctt
    21361 acaagtaatt atcaaaaaga aaaccctcta aaagcgccta tgttttgtat gttacttaga
    21421 aaatacatac aaggtggaaa tatagtagaa atttctcaaa taggttttga aagaataata
    21481 aaaataagtg ttgaatcttt agatgaatta aaagaaaaga cagttaaaaa tattatgatt
    21541 gaaattatgg gaagacatag caacataata ataactcatg gtgaagaaaa taaaattata
    21601 gattctataa aaagagtccc ttttagcata agtagagttc gtcaagtttt acctggtcat
    21661 gattattcat tgccaccaga acaaaacaaa ctcaaccctc ttgatgatat atctaaggat
    21721 ttgtttatta aaaacctaga ggaattggaa ggacctatct ttaagtctat atattctaaa
    21781 tttctaggta taagccctat tatagctaaa gaaatttgct acagagcagg tgtaaatcaa
    21841 aatgctatca taaaggatat atctgatgaa caatttgatg ctttacacaa agtattttgt
    21901 aatttattca atgatataaa ttctaacaaa tactctcctt gcattattat agataaaaag
    21961 gtggataagg tagttgattt cagttgtata aaccttacat tatttagcga tttatcatat
    22021 ataaataagg atagtatgtc tcgtattctg gaagatttct atagaactaa ggatattaaa
    22081 gatagaatta atcaacgttc atctgattta aagaagagta tatctgttaa gcttgataga
    22141 ctttataata aacttaaaaa acaagaagaa gaattatcag aatccgaaaa tgcagacatt
    22201 tataagataa aaggagaatt gattacttca tatatttata tggtagaaaa aggtatggaa
    22261 agtattgaag ttgctaactt ctatgatgaa aattgtaatg atgtaacaat agaattaaat
    22321 aaaaacttga caccatctga aaatgctcaa aaatacttta aaaagtataa caaaatgaaa
    22381 catgctaaag tcgagatatc tcatcaaatt tccttaaata aggaagaaat agattactta
    22441 gaaaacataa ttttaagtat tgaaaactgt gaaaatttag ctgaattaca agatataaaa
    22501 gaagaacttg ctaaagttgg atacatcaag actcaaaaga aaaacagtaa aaaggatact
    22561 atcccatcta caaaacctca tgaatttgtg tcttctgatg gatttaaaat attggttggt
    22621 aaaaacaata agcaaaatga ctatcttaca ctaagacttg ctgataatga tgacttatgg
    22681 atgcatacaa aaaatattcc tggttctcat gtaataataa agtgtgctgg taaggaagtc
    22741 cctgataata ctgtatttga aggagctatg ctagcagcat tctttagtaa gtcaaaactt
    22801 tcttctcagg tgcctgtaga ctatactaag agaaaaaatg tgaaaaaacc tagtggttct
    22861 aaaccaggta tggttattta tgaaactaac agtactattt atgttactcc tgaagaagaa
    22921 acagtagcca agcttaaggt taaatctgaa taaattctgc actaaataca aaataaatgt
    22981 tagacaacta cacattatca ttctaaataa aaatagtggc cttcttataa agtacaataa
    23041 taaggccact ttctaattag cttactttta attttctaac aaaatacttt tctgttttaa
    23101 taactatact tatattccca acaaatattt tcagtcttct tatatagatg taattaaaaa
    23161 gttaacacta aaagctattt tttaatttat tgatttgttt tagtaattca ttatattctg
    23221 attcatacaa ttcttttttt agattatctt tttctataga taacatagat ataacagttg
    23281 taagtctatt ttctaatact agaagatttt cttttttgat ttggttttct ctactaggag
    23341 tttttttatt tctgctaatt gtatattcgt cataattaca atcaaactcc cttattttag
    23401 catcttttat ctctataata tagttacaaa tatgtgatac aaatactcta tcatgagaca
    23461 ctataagcat agttttatca gtatttatca gtgccttttc cagtgcttcc atagatttta
    23521 tatctaagta atttgttggt tcatcaaata ctaaaaaatt attatcttcc aatattattt
    23581 tacatagtgc tatttttacc ttttctccac cactaagtac ttttactttt ttgtagacat
    23641 catcaccttt aaacccaaat aggcttaagt ttatccttat aaaagattca tcaaatgaac
    23701 tattaacttt agtattgtat agaacactct tttcctcatc taataaactt tgattttggt
    23761 caaaataacc tacttttacc ttgttattta tttttatatt gtcattttta tcagccaata
    23821 tttcttttat cagagtactt tttccacacc cattatcacc taacaatgct atctttttac
    23881 ctctttttat cttaaaggaa acattatcta acaggagctt attttctaac tttagagtca
    23941 tatcttttac ctctactaga tttttgctaa ttatctccat accatcttgc atcttaatgt
    24001 tcatttcttt agaaacttta ggtttattct ttacctcaag tttatcaatt ctgcttttta
    24061 tagccttcac atttgagtcc aactttttct tattagtctg accacccatt ttgtgcaacc
    24121 tagcttctga attacccatt ctttttggtg tttttcttat gccatcactt aaagcttttt
    24181 tatataaaag agctttttca agcctttttt tctctttaac atattcttga tactcaaaat
    24241 cagctctttt tacttcttca tctttttgca ttaaataatc agtgtaattg cctttgtaag
    24301 cttttagttt tccatcttct agttctaata tagttgtaca taactcatcc agaaaacgtc
    24361 tatcatgaga tattaataat aatgctccct catgcctttt aaacatatct tccaaaaccc
    24421 caatgctttt tttatctaag tttgaagttg gctcatcagc tattatgata tttttcttat
    24481 tactcagcgc atctgctatt tttaacttaa ccttttcccc tcctgataag tgttcttcaa
    24541 atttatctgg agcatttaaa agactcttct ccttactatg actacatgtt tcaatattat
    24601 tttcactctg acttatataa gaatagcttt ctgtaaggta agtatatcct tcatcaattt
    24661 ctatttctcc caatattgct tttaataatg ttgtttttcc ttgaccattt gaaccaacta
    24721 gacctatttt gtcattttct aatatttcaa gtttatctat atccaaaatt aatttatctg
    24781 cataatattt ctttaaattc tctactttta ctaacaacat aaaaaatccc tccttaacac
    24841 acttaaatat aaggagagat tctataaata ggcataacaa ataaacgtat agactataat
    24901 ctactctcct taatttaagc attgcaaata aagccaccaa aataggcttt tcaaaaatta
    24961 caatacttta aattataaaa aatagattat attctcattg tcctagttac cttgcccttt
    25021 ctttcattta tttttaatca ttatatcaac attaattaca aatgtctata gaaaattaac
    25081 taattttagt tattcaatat acaacttatt ctttaaattt attcttcttc aactttaaag
    25141 tcctgattat ttaaaattct gtttaataca attccaacaa ttgcagctaa actaagacca
    25201 gttatcttta ctgtacttgt tatagggatt ccaacagaaa tacctttatt agataaatat
    25261 gttgttccaa gacctattaa taatataact gctatgatta atatatttat tttactttct
    25321 acacaagaac tgtcccttat agtttttaat cctacataag ttatcatcga aaatagcatt
    25381 atactaattc cacccataac tgaacctggt atactttgaa gaaatccacc aaacttgcct
    25441 acacatgcaa gcaatatagc aaatattgct gtacgtctaa gtattgaagg gtcatagttt
    25501 ttagttatag caagtactgc tgtattttct ccataagttg tattggctgg tcctcctaaa
    25561 caccctgcaa ctatagtcgc aaatccatcc ccaagtaatg ttctatttaa tccgggattt
    25621 tctataaagt ttttacctac tacagcacca ttagtagtca tatctcctat atgttccata
    25681 aatacagcta atacaactgg agatataatc actattgctc ctaaactgaa cttaggaact
    25741 gtaaagttag gtattgcaaa taaacttgct tctgtaatag tagttatatc tactaatctt
    25801 aatatcaaac agattatata cccagaaaaa actgctatta atattcctaa ttgttttata
    25861 aatcctctac caaacttatt ggttaaaatt gctattgcta aagttataaa tgctatcaca
    25921 aaattggctg aagccatact aaaagctgtt ggcaataaat ttaatcctat aactactatc
    25981 atagctcctg taacttgtgc aggaaaatat aatttaattt tatttacacc aactactttt
    26041 actataatcg acattataat ataaattaat cctgctacta ttataccacc ttgagcatat
    26101 gctaaatctc caccatatgc ctctttagct gctagtataa ctggtataaa cgcaaaactt
    26161 gaacctaaaa aggcaggtac ttttccttct gtacaaaaat gaaatatcaa tgtaccaaca
    26221 ccagcacaga aaattgcaac tgacggatta aaacctgtca aaatgggaac taatactgtc
    26281 gctccaaaca tggctaatag atgttgtaaa gacattatcg tttttttcat aaaaacctcc
    26341 aaaattatta tatataaata aatcctctgt ttaattaata tcttttaatt aattatcatt
    26401 ccctagattc tattttttaa cctctatata cttattatat aatttatcat tattttacat
    26461 tttacgacat caaaaactca aataaaggag aataaaaact acattaagac tcacatccat
    26521 aaaattataa tatttatttt gtgcaatcta tgtaagcttt atcctcctcc ctgactattc
    26581 aatgctaagc ttttatataa tacttaaaaa taattattct ttgattgtaa ctgaatcttc
    26641 tccatcaatt tctaataaac tgacagatat aatttcatgt ctagaagtag gtacattttt
    26701 tcctacataa tctgctctta taggcaactc tctatgccct ctgtccacta gcactgctaa
    26761 ttgtattgat ttaggtcttc ctatatctat aatagcatct agtgaagctc tcacagttct
    26821 tccagtgtat aaaacatcat caactaaaac tactgtctta tcatttacat taaaatctaa
    26881 ataagttcct ttaactacag gttcaacatg aattttttcc aaatcatctc tatacaaagt
    26941 tatatcaata tctcctgtat ctacttttgt tccttctatt tgttctattt tcttagaaat
    27001 tctatttgca attggcacac ctctagtctt tattccaact aaaactagat tttctacacc
    27061 tttatttctt tcaattattt catgacttat tctagtaatt gctcttgcta ttgccttttc
    27121 atccattaat tgagcttttt ctaccatatc ataattattg tcaaagatat aatatatcta
    27181 tttgacaaat ctactccttt cttttaattt tttataaatt tatttttatt taaaatttca
    27241 atttaaacaa caaaaaaact tctcatcaca agacaagaag ctcttatata aaaaatatac
    27301 agtgcaattt agcaattgtc ttacgatatc tctgtatcgt ttaaagataa ttttttaatc
    27361 tatcaaatca aattatatat ttatatttca aatttttcaa gcttttttaa acaaaatact
    27421 ttaatataag atattttatt taaatttcac taaatttatt tacttatata aaaacaaaaa
    27481 atgatttata agtattatac aaatagttat tttatctttt taataacttc cttaaaatac
    27541 tcaggtaatt ctgaattaaa ttcgatatat tccttagtag taggatgtat aaatcccaac
    27601 ttcttagcat gcaaagtttg tccttttaat ttaaatttag tttcttttgg accatataca
    27661 taatcaccta aaagtgggtg atttatagac aaagcatgaa ctcttatttg atgagttcta
    27721 ccagtttcaa gcctagctct catatgtgta aacttatcaa atctttctac aacttcaaag
    27781 tgagtgattg cattttttcc atcttttact acagccattt ttaatctatc cttaggattt
    27841 ctccctatag gtttatcaac agttatttta tcttctttaa ccacaccata gcatataaat
    27901 tcatactccc tagttatagt atgttccttt aactgttctg ctagaaaatt atgtgcataa
    27961 ttgttcttag ctatcataag aagaccagaa gtatctttgt ctattctatg aactatacca
    28021 ggtctaataa cgccatttat tgatgacaac ttatctttac aatgatataa aatcccgttt
    28081 actaacgtat tttcatagtt tccaggagca ggatggacaa ccatatcctg aggtttattt
    28141 attattaaga catcatcatc ttcatataca atatctattg gaatatcttg aggtaaaact
    28201 tctaatactt taggttctgg tatctctata acaatattat cttcctcttt aaccagatat
    28261 ctagcttttt ctactttatt attaaccttt acttttccat cttttataat tttttgtata
    28321 taacttcttg acatatcacc taattgttct gctagataaa tatcaagtct aacttcttct
    28381 tcctcttcaa gtactaaaaa ctcttttact tcatccatta tatcacctac ttttactttc
    28441 aaaaaataaa acatatatac ataaaaacac agttcctaca actacaaata catcagctat
    28501 attgaataca tattcccata taattctaaa gtcgaagtaa tctactacaa aacctaatcg
    28561 tactctatca attagatttc ccaatgcacc agatataatt aatataattc ccaaccttcc
    28621 aaatatatgt actttccttg tattaagata gtatagtcca aatactgttg caagtaatgc
    28681 aacaattata aatatccatt gattattttg taataatcca aatgctgcac ctctattttc
    28741 gacataagtt aaatgaaata cattatttat tattggtatt gttgatacat ccaccaaatt
    28801 attcaatacc catattttag acagttggtc taaacctatg agtagaatta ttattaatat
    28861 atatagcaat gatttttccc cctataaatt atttaaaaac caattttatt tgaagcacac
    28921 tattattatt atacaaaaaa aatgctctaa agagcaactt tattaaaatt gtattttctt
    28981 aataaagcta gaacaaaatt taaaatagta ctactttttt tatattgtag atttctacat
    29041 atttaaaatc atatcattat gtataaaaaa tttaaaaata ccatactaat caatattttt
    29101 tgtgtatttt atagattaaa gattaatgat gtctgtaatt taaacttaca atcaccacta
    29161 atcttcaaac aaatattttt acttttgcac ttgcttattt ttatctaaat gttgtataag
    29221 ttcagatact gttacgaatt tatatcctct ctcttttaat ttaggaatta ctacttctaa
    29281 agcttgtata gtttgtgatt tatcatttct tttagtgtta taatcatgta aaagtattat
    29341 attaccattt tggacctttg taaggatagt atttataatt gcatctactc cagggttaga
    29401 ccaatctcta gggtctaagt ttgaccaaag aattatattc atgcctttag aaactataat
    29461 atcacagaca cttttactca tggctctata tggaggtcta aatatctttg gttctactcc
    29521 tattacactt tttataatat tttgcgtatc tgttatttct ttttcaacac tactataact
    29581 attttttgca acatttatat gtgtaaatgt atgattccct atttcatgcc cctcatcata
    29641 ttgtcttttt aataattctt tgttatattc tactttttct ccaactacaa aaaaagttgc
    29701 ttttacatca tttttcttta atatatcaag tacttgtggt gtaaaatcct catctggacc
    29761 atcatcaaaa gttaatgcta aaattttttc atcctcattt ccttttttta ttattacatc
    29821 ttcataatcg tcttttatat tgtcatttaa actatatgct tcttttgaac tatcttgatt
    29881 atgtgtatta tgattatatg tatatacacc tactatagat aataaacata tacatatacc
    29941 ccatattatt ttcttcatgt cattacctct tttctaattc attctagcaa tatattttat
    30001 aatgtcagtt ttttaatctc tcaatattcc atttttattt aaatcagtta aatttgttag
    30061 gtcttctgaa taaaagttag aatcttctac atcagggttt tgcattgcat cacctaataa
    30121 attattttct ttctttgcac aatcattaca taaacttgta tctggcaata tatccaatct
    30181 ttctaaatct atttgattat gacataaatc acaaatccca tatgttccat tctctatttt
    30241 agataatgct aagtctattt gatacaatct tccctcttca tgatttatta gactgttttg
    30301 catattttgc atatataaat ctgttcccat gtctcctata tgattatcat aactagataa
    30361 ttctcctgat gaatactttt cacttgtatg tttagttgta tctccaaata aagtattatc
    30421 tttcatatcc tctactaaat tagttaaatt ttctctttct ttcaataatt tatctttata
    30481 ttttttagta tccattttta acacctctaa ttttaatatt tacatcataa aaatagtatg
    30541 tggaaaagta aattaaacat acaataaaaa taagatttga ttcgaaaaat ggaatgtctc
    30601 aaaatgaaat ttttagttca tttaaccctc ttttttatat gaaatcaaat atatttttat
    30661 cctttcagca ataaaaaaga gacttatctc tattttgaga tagtctcttc tttgaacata
    30721 atttaaatac aattttttaa tttttatagt tattatttta ctaacttgcc aatttatgat
    30781 tattttgttc tttaatatgt tttgcttttt taaagaataa aagaccaaga attattgcta
    30841 caataacacc tattccatta gatatattaa ctggtagtct aaatccttct tttgcttgca
    30901 ttatataact aaatgttact acagtcataa aaagtgctgg taatgtagtt atataatggt
    30961 ttttatttgc tttgactaaa tatgcagaac cagtccacaa cattatcatt gcaagtgttt
    31021 gatttgccca agagaaatat ctccaaatta tattaaaatc tataaatgta agagcaacac
    31081 caactgcgaa taatggtaaa gctattaaaa atctattttt gactgcatct tgattataat
    31141 tcattgcatc tgctattgtc agcctagcac ttctaaatgc tgtatctcca gaagttatag
    31201 gacatgccac aactccaagt atagctaaag ctcctccaat aggccccatt aatccaacag
    31261 atactgtatt tactactgtt gctgctccac catcattaaa tatcacatta agttgaggta
    31321 tgccatggaa atatgacatg gctgctgctg cccaaactaa agctattata ccttctgcaa
    31381 ccattgaccc ataaaatact ggtcttgcct cactctcttt ctcaacacat ctcgccataa
    31441 gtggtgattg agttgcatga aaacctgata ttgctccaca tgctattgtt ataaataagt
    31501 atgggaataa aggtgttcca tttggattca tattttgtaa atttatattt ggtatgttat
    31561 acccctttat tattaatcct ccagctacac ctactgccat aattaataat gctgctccaa
    31621 ataaaggata tattttacca atcaatttat ctattggtaa aacagtagcc ataatataat
    31681 aaagtattat cactccaacc catatttgtg cgtttacacc tgtcaatgtc tttaataatc
    31741 ctgctggacc tgttacaaat acaactccaa ccaatactaa aagaataaca ctaaatattc
    31801 tcattgctat ttttgcacca tttcctagat aaaggcctac caactctgat gcagatgtac
    31861 ctttgttttt cattgataga tatcctgaag caaaatcgtg tactccacca gcaaagattg
    31921 ttccaaacac aatccataaa aatgctgatg gtccaaataa tgctccttgt atagctccaa
    31981 atatcggacc taaccctgct atatttagaa actgaattaa aaagattctc ttccatttca
    32041 ttggcacata atctactcca tccctacaag ctatagctgg agtttgattt ttgtcattgg
    32101 ttccaaaaac cttttctaca aaagtaccat agacaaaata acctactata agtactacta
    32161 ttgaacctaa aaagcttacc ataaattcat ccccttagtt tttatataat tgctttataa
    32221 atttttatga gtttaagaaa actatttcca gttcaaatag taatactttt ttatgctaat
    32281 ataaatatta attacattaa gtgcatattt aagtacatga aatgacttta tatatgcatt
    32341 aacagcctaa attcttttac tttgcttcta cttacagtta cttcataatc actatttttt
    32401 aattttaata tgtatgtatt attaaaccat ggtataattt cttttacttt ggttaagttt
    32461 acgatataag acctatgtgt tctaaaaaat gttttatcat ttataagatt ttcgacttct
    32521 gatatgcctt cttttaatat aaacttttct ttctcagtat atataaatgt ctgcctttca
    32581 ttagcttcac agtaatatat atcatcaata tctattacaa ctaatttatc acctttccaa
    32641 aggctaattt tatgtgttat ttcttctatc tcctgattta ctgcttcctt cttatatttg
    32701 tataaattac tatttacatt aaatagttca atatcactca tctcactttt ttctaattta
    32761 ttcagcatag aaataattct ctcttcagaa tatggtttca atatataatc aaatacatcc
    32821 aaagaaaaag catctactgc ataactttca tatgctgtta taaaaacaat tttaggtctt
    32881 gatttaaact gatttaatgt ttttgcaagt agcattccat caagatgagg tatatttata
    32941 tctaaaaaaa ttacatctat tttattttcc tgaataaagt ctaacacctc tataccattg
    33001 gtaaattcac ttactacctc gattccactt ttattttcaa taaaatatct caattccttt
    33061 cttgctggaa attcatcttc cactattata gctctcatac ttctactcct ttacataaaa
    33121 tactatcaaa gtcccattat ctattcttct tatattaagc cctcttccat acatcagttt
    33181 aagccttaaa tgtacattat aaagcccaat cttattttct ggcatattat cttgatatac
    33241 attatttata atttcctcac ttatacctat accattatcc tctattgata ttctcacagt
    33301 attttctaat gaatacggta acttttttat ggatattttc actacaccag ctctaccact
    33361 ttctaaaata ccatgtatta ttgcattttc cactaatggt tgtataatca aacttggaat
    33421 ctttatattt acatcttcta tatcataaga tacagttagt aattctccaa acctagcttt
    33481 ttctatctct acaaaagatt taacttgttc aatctcttta tttatatcaa ttaagttatc
    33541 actatattca agattatatc ttagataagt tgataaattt attattaact ctctagcctt
    33601 atctggacta aatcttataa atgaagctat tgtgtttagg gaattaaaca aaaaatgtgg
    33661 attaatttgt ctttgaagtg cttttatttc tgctttattt ctcgcttcta tatctttttt
    33721 atctttctca atcccctcta ctatagatat aacaaatcct attccaatct gcccaactat
    33781 taaaggtaaa tatattccac cgattatttg aattgctaaa gaatgtggat atgatattaa
    33841 gtatataagt aatatagtaa aactttcaga aatcatgcct accaatattc catacattac
    33901 tctatgttgt tttggtatcc tcttatatag aaatcctgat aatacacctg ctagtatact
    33961 tgataaaaga catggaacag atgttatccc cccaatatcc ataaaatatc tatgaatccc
    34021 tgaaaaaact cctgctgtaa tacctaccat cggaccaaat aaaatgccac ctgatactat
    34081 agatattatt ctagtgttta ctattgaccc catataattt ataccactat aagttccaaa
    34141 tatggctaaa aaagtaaaaa ctaatgctat acagaccaag tcattaaaac tatattcttc
    34201 cttttgaaat atctgcttaa atatttttaa ctttgttatt agaagaaaca atactacaat
    34261 aaggcttgtc ttttctatta atgcgattaa aattgatgtt ttcataatct ccctttaaaa
    34321 aataaatttt tacacactct aaatttatta tggttgttat aaattacata tttgaagtaa
    34381 tcctatcaca aaactttcca catttaatct tttaattaaa taagaaatag ttgtacttct
    34441 attaacaact aatactagct agcataagct tgtaataact tgtagttatt attaacttta
    34501 caattctagt atttaatttt atactttaga aatctctttt cttagaaata atactttcga
    34561 atatcatata atacaatatt atgtagattc ttactcatac aacatatttc tttaaaaatt
    34621 ttatactatt ctttgttcaa atatctcatt tgatttaatt gtgttaaaca cacattatca
    34681 tctctctgtt atatatttat tatatagccg tatatcgata aagtaaataa atcccatatt
    34741 taaaataata tgtttgacaa taaattatta taatgtatat ttaccataat aatttgtcaa
    34801 ccttgaaagt tacttctata attattgtta aataattctt ctaaa
//
LOCUS       DAEMGG010000027        34726 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEMGG010000027 DAEMGG010000000
VERSION     DAEMGG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.027"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="KPK24_001323"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="KPK24_001323"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG2299008.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="KPK24_001324"
     CDS             complement(2851..3216)
                     /locus_tag="KPK24_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2299009.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="KPK24_001325"
     CDS             3415..4263
                     /locus_tag="KPK24_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299010.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="KPK24_001326"
     CDS             complement(4700..5788)
                     /locus_tag="KPK24_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299011.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="KPK24_001327"
     CDS             5913..6641
                     /locus_tag="KPK24_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG2299012.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6843..7334)
                     /locus_tag="KPK24_001328"
     CDS             complement(6843..7334)
                     /locus_tag="KPK24_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299013.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7345..7788)
                     /locus_tag="KPK24_001329"
     CDS             complement(7345..7788)
                     /locus_tag="KPK24_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299014.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="KPK24_001330"
     CDS             complement(7778..8617)
                     /locus_tag="KPK24_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299015.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="KPK24_001331"
     CDS             complement(8836..10221)
                     /locus_tag="KPK24_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2299016.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="KPK24_001332"
     CDS             complement(10394..11137)
                     /locus_tag="KPK24_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG2299017.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="KPK24_001333"
     CDS             complement(11157..12419)
                     /locus_tag="KPK24_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2299018.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="KPK24_001334"
     CDS             complement(12603..13601)
                     /locus_tag="KPK24_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG2299019.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="KPK24_001335"
     CDS             complement(13585..15096)
                     /locus_tag="KPK24_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG2299020.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="KPK24_001336"
     CDS             complement(15150..16112)
                     /locus_tag="KPK24_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG2299021.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="KPK24_001337"
     CDS             complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="KPK24_001337"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG2299022.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18105)
                     /locus_tag="KPK24_001338"
     CDS             complement(17092..18105)
                     /locus_tag="KPK24_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2299023.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18333..19310)
                     /gene="bioB"
                     /locus_tag="KPK24_001339"
     CDS             complement(18333..19310)
                     /gene="bioB"
                     /locus_tag="KPK24_001339"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG2299024.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19516..19779)
                     /locus_tag="KPK24_001340"
     CDS             complement(19516..19779)
                     /locus_tag="KPK24_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299025.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19849..20922)
                     /locus_tag="KPK24_001341"
     CDS             complement(19849..20922)
                     /locus_tag="KPK24_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG2299026.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21181..21939)
                     /locus_tag="KPK24_001342"
     CDS             complement(21181..21939)
                     /locus_tag="KPK24_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2299027.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            complement(21936..22853)
                     /locus_tag="KPK24_001343"
     CDS             complement(21936..22853)
                     /locus_tag="KPK24_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299028.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22853..23053)
                     /locus_tag="KPK24_001344"
     CDS             complement(22853..23053)
                     /locus_tag="KPK24_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG2299029.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23058..24152)
                     /locus_tag="KPK24_001345"
     CDS             complement(23058..24152)
                     /locus_tag="KPK24_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2299030.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24662..24802)
                     /locus_tag="KPK24_001346"
     CDS             complement(24662..24802)
                     /locus_tag="KPK24_001346"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG2299031.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            complement(25112..26419)
                     /locus_tag="KPK24_001347"
     CDS             complement(25112..26419)
                     /locus_tag="KPK24_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2299032.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIVHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26484..26963)
                     /locus_tag="KPK24_001348"
     CDS             complement(26484..26963)
                     /locus_tag="KPK24_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG2299033.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27010..27873)
                     /locus_tag="KPK24_001349"
     CDS             complement(27010..27873)
                     /locus_tag="KPK24_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2299034.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27873..28628)
                     /locus_tag="KPK24_001350"
     CDS             complement(27873..28628)
                     /locus_tag="KPK24_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2299035.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28655..29143)
                     /locus_tag="KPK24_001351"
     CDS             complement(28655..29143)
                     /locus_tag="KPK24_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2299036.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29161..29562)
                     /locus_tag="KPK24_001352"
     CDS             complement(29161..29562)
                     /locus_tag="KPK24_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG2299037.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29565..30062)
                     /locus_tag="KPK24_001353"
     CDS             complement(29565..30062)
                     /locus_tag="KPK24_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2299038.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30086..30490)
                     /locus_tag="KPK24_001354"
     CDS             complement(30086..30490)
                     /locus_tag="KPK24_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2299039.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30720..33287)
                     /locus_tag="KPK24_001355"
     CDS             complement(30720..33287)
                     /locus_tag="KPK24_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2299040.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33453..34196
                     /locus_tag="KPK24_001356"
     CDS             33453..34196
                     /locus_tag="KPK24_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG2299041.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT        11254 a         6357 c         3560 g        13555 t
ORIGIN      
        1 cttaaaaata attcgtcaat actttttaaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaacgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatacttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaaaaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctagaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgttaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcatgcc taacaatgtg cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggca tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctgtac aaggtatacc acaagctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtgttgg tgattcagaa
     4501 tagtatggta ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gcaaaaaaat
     6301 ataatgtaac cgattttcta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatgtttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagtttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaaaccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgatcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaat cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcatagttca aagaaaacct agagatttct ccatgatttc
     8521 tttttaagta tttacatagt ccacaatagt aagctttgta ataatcatat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttatgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttgttta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcctt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg tagctaacat ctccaatatc taatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg cacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg gtattcttat tggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcttct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta ccaagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttagta gtggatactt tttgagacaa
     9961 accatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccataact
    10021 agccacaaat gctgtatcca taccttctaa tccctctata ctaaaaccat gtttttttaa
    10081 acattctttt tgtaaatttg aagcatagta ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattatcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctacc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgttcag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccaaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaaccc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattac tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 ttttttccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gaccataaag ttttccatta tcttctgtag cacgatatgg attatatttc
    12061 cacttactta tatcacccgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatccacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattct cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaatat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcctcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcacc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattttgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatattt ttcaacatct tctttataag
    16201 gcataccaga ttgtgcacct agtttagtta cagacagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tgtttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattgcact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg tagccttctt cattagcttt gtcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct tttttccatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgaa aaaaaaatta atatactttt ataattatct
    18301 aaaagtatat taattaattt aagcagtatt tattaaagtt caaatccttg ctctataatc
    18361 atttttttat cattttcaat cccacttcca acagttgtca aataatcacc aacaattgca
    18421 gaatttactc ccccattaaa tccaactttg tcatatccct ttaatgatag ccttccacca
    18481 gcatacctta tgtacacatt aggaattata aacctaaata ctgctgttgt ttttagtatt
    18541 tccattggtt ctaatacatc ataattttcc attggtgtat tcttaatagg atttaaaatg
    18601 tttattggaa atgattttac atttaattct cgtatttcaa aagccatttt tagtctctgt
    18661 tcttcactct cattcattcc aattataccg cctacacata catctagccc agctttttta
    18721 gcatctttta tagtttttat cctatcttta tatgtgtgtg ttgtacatat attatgaaaa
    18781 ttatctctac acgtctcaac attgtggtga tatgtagtca cacctgctga cttcaacatt
    18841 ttagcttgct cataatctat aatacctaaa gatgcacata attttaaatt agtattttcc
    18901 ctcaatcctt catagatatt taaaatatta ttaaactcct ttcctgacat acctttacca
    18961 cttgtaacca atgagaatct atgcactcct ttactctcca tctctttagc tctatttaag
    19021 atttcattgt aatctaataa tgaatattct tctacaccag ttttataata agaagactga
    19081 gcacaaaatt tacagtcttc actacatttg ccagatttag cattcattat cgtacataaa
    19141 tctgcttttc ttcccataaa atattctctt atttcatttg cagattttaa tagaatatca
    19201 aaatcgtttt tattatttgt atcaaggctt attaaattaa gagcttcttc ataacttatt
    19261 tctttttctc tcaatacttt attttttaat tttattatgt atttttccat atatcctcct
    19321 atttgtcaac ttaatatttt tatgtagttt acttattata tatagtattg catatataac
    19381 atttattggt caactttatt ttagacacaa gtttactata cacaaaaaaa tactgcttca
    19441 aaacaatatt agtatataga ttatacatat tattattaaa gcagtatttt ttattagaat
    19501 ataattctta ctggtttata aaactatcct tgtcttaact ccatccaagc tttccattgt
    19561 aataacttct aagccttcat attctctaaa tagccatttt aaatccttca tggcttgatt
    19621 aacctgactt tttgttagat tatttataaa atattctttt ctagtttcct catactcttt
    19681 gctagtcaaa aagtttctta gaaaattaaa cgcaaataca cttggaatat tgtttacatt
    19741 agccttcttg ctgttttcct tctcatagta aattttcata aatgcatact cctttgatac
    19801 ttttagattt ctcttacaat aattatatgc atttaagtgc tactattctt ataattttat
    19861 taattcatat tctttacttc ccatacccat ttcacatgca tagtctatta ttctagttcc
    19921 atttacatga ggatgttcat gtttgaaaac atcatgaccc atttccttac aaatcaaatc
    19981 atagcatgct tgttcaatag ctacagggtc tgttgatgct aatattccta tatcatgggc
    20041 aattggtcta cctgaccaag gtacacaatc acaaagtgga gttacattca taacaaaagt
    20101 tatataccct accttgtctt ttttatttga tactgctcca taagcgtatt ctgccatctt
    20161 ttcgacaaac acatctgagt cactttccca ttgtatagtt actgctctag ttggacagac
    20221 agttggacat tctccacatc cataacatac atcagaatct ataactgctt ttttatccac
    20281 tatacttata gcttttgtag gacaagaatt aacacacttt ccacaaccta cacacttctt
    20341 ttcctttact actggagtaa catcagaatg ttgcatttgt tttcctgctg cacttgcaca
    20401 tcccatagct aagtttttta atgctcctcc aaaacctgct gcttcatgac ctttaaaatg
    20461 actcattact atcattgcag aagaattata aatttctcca cctattttta cactttcaaa
    20521 atgttttttg tctattttta cattctcata atttctacta tataatccat ctgcaattat
    20581 tactggtgca tttacaactg cataagcaaa tccattttct atagcagttg taagatggtc
    20641 tacagaattt gttctactcc ctgtatacag tgtatttgta tctgttaaaa atggctttcc
    20701 ttcgcagtct ttaaccttat caaccacttg tctaactgcc acaggactca tatatgtagt
    20761 atttcccttt tctccaaagt gaagtttaat tgcgacttgg tcattttttt ctattaaatc
    20821 tttgaatcct gcttcatcaa ataatcttct tacattgttt ggtacgtttt tattttgcga
    20881 atttgagtat aaatcacaaa aatatacttt tgacttttcc atatctacac ccctttaaaa
    20941 tatttactta tactaatttt atcactttta aagtaatttt tagtatattt taacataatt
    21001 aaatattctt atatttattt gatacaaata atattatttg tattattttc tacacttaat
    21061 tctatatatt aaactacacc taaaaatcaa aaagacttga aacttaagtc caagtctttt
    21121 tgatttttag gtgtagtttt taacaacata agattacttt accttgtgat aaaggtatta
    21181 ttaaactggt tttttattaa ataaaataac agctgctagt aaacatacta tagaaataag
    21241 aactgttata ataattggaa taataaattc tggcattgcc actttccctg aaatcaagtc
    21301 tatatttttt gacatcaaga aattaggagt ataaggtgca atctgtttag gaatagaaat
    21361 taaactcaat aagagagttg ctaccaatac aagtaaaaca ctggcaaatg ctggtctaaa
    21421 tagtacacac ccaagaatta atacactaat atacataatt cctataagcc ataaattaaa
    21481 tgctgaaaat ataatatgat taagatttgc agttggccaa aaatacattg tatagccata
    21541 agcaaataaa aaactcaacc aaaaaccaat tgtcataata atcaccgtaa cagagaattt
    21601 agacaatact atagatgaac gtgataaacc tttagtcagc ataataacaa gtgtcccttt
    21661 agaatattca tttgataaaa tattacaaaa aataattaga gttaaactca taccaagacc
    21721 agagatattg ctaaaaaatt gtgtccaaga atctaaagca gttggtgcag cagtaacttt
    21781 taaagtcggt gcaaaatgag caatcaagtc tggcataaat tttgcagaaa taggactact
    21841 tataccaaat attagaaata aaataattaa actaaataat ttataatttc ttaaattctc
    21901 tttaaattct ttttttgtaa atgctatgta ttctctcact ttactacctc caaaaataaa
    21961 ttttcaagtg ataattctat tagttccatc tttactggac aaagattcat ttctgcgagc
    22021 gtagatataa ctgttttttg tattgctttt atatctttac catgcaaaac aatttccata
    22081 ttagtttctt ctgaatcttt tagtaaggat ttaataccat cactagactt aaacttttga
    22141 atttcatcat tacttgcaaa ctccagtaaa agtctatcct ttccgtgcat actttttatt
    22201 tctgaaagtg taccactaag ggcaatgctt cctttgttta atatagcaac atggtcacag
    22261 attctttcaa catctgatag tatatgtgtt gaaaatatta cagttgttga gtctttgatt
    22321 ttaagcataa tatctaatat ttcttttcga ccaacagggt caagtgcact tgttggttca
    22381 tcacaaataa gtaatttggg gcgtgaaagc aaagcttgag ctataccaag tcgttgcttc
    22441 attccacgag aaaacccacc tattcgtctc ttttcatttc taagacccac aagtgataac
    22501 aactcttcac tccttatctg tatttctttt tttgaaagtc ccgtaatttc accacaaaga
    22561 gaaagatatt caagtggtct catataattg taaaactctg gaacatcagg cagataacct
    22621 acatgacgat tagaacttgt tttcccatag gtcactttct caccacaaac atctatagtt
    22681 ccactatctg gtttaagtaa cccaagtacc attttcattg ttgtagtctt acctgcacca
    22741 ttcttaccta caaacccaaa gactgaccct tctggtacag taaaatttaa attatctatt
    22801 atctttcgat taccaaaacc ttttgataat ccatttattg agacaatatt catcattctt
    22861 cacctcttcc tattgtaaaa tacaaaattg gtcctattaa actaataaac agtactacaa
    22921 taatccatat tgccttattt ccaattttat aatttggatg tctcaacaca tgaacaagtg
    22981 cagtaattat aagtattaaa tctaaaataa taacaggaac caaaagtggt aagtattcca
    23041 tcaaattttc cataatctta ttcctccaat aaagataatt tttctataag aaatttgtat
    23101 tctttgtttt cacttaatac agaaaacatt ggattatttt taatagcagc aacaatacct
    23161 gcttttacga ttgtatcatc acgtgtaata cctgttccaa tttcagaaat ccatctgtca
    23221 agtttattaa aaaagtcatc cccatgtatt ataattgggt atatatcacg tgttgctaaa
    23281 tctacatatt ttcgtaatga tttaatagct ccttcattat caccatgtat taaattaatt
    23341 tgtgcctgtg ttaaatatga agaaagcata atagcaggag ataatgtatc aagctcaaat
    23401 atatctgaaa ttgctagaat acgtctttct atttccttca tttgtaattt atctgcattt
    23461 tgcaaaatac ccactagatt ttggactaat gatataaggt tctgatatgc accaagttga
    23521 aatgtttctc ttgcttcatc catttgacca ttcatcattt gcccttgtgc aagaagtata
    23581 gattcattta tcatgggaaa attgctgttt tgcaataaat caatcacttg tatgggttga
    23641 tttaacaata tataacaagt tgcctccata ctttttgctt gacggcaaat atctatgtca
    23701 ttacaagttt cctgtatacg aataaatatt tctaatgctt cactaattaa tagttcacgc
    23761 tttttttcat ctacaatatc atagtgatta actattaaaa gacccatccg aaaaatcaaa
    23821 ggaaaacatg aataatattt ttttatgatt tctcttattt catccatgac ttcatcaaat
    23881 ggtttaaccg taaagtcttt gcacagttta ttatacagtt tattaatatc ttccttcatc
    23941 atttgaggtt cataacatat taactcatca acggtaatgt tgaaatatgt cgcgagttgc
    24001 ggtaaaaagg ttatgtcagg atagctttgt ccagtttccc atttagatac agaagcttta
    24061 gaaacgccta tataatttgc aagttcatct tgtgtaattc ctttttcttt acgctttaaa
    24121 atcagtgttt ttgcgatgtt taattcattc atgtcaatcc ctccttatgt ataattatat
    24181 ccttaaacat gtaaagctaa caagcgattt gaagttgact tttgacgaaa aaatcaacca
    24241 cagagaaaca aataaacaag tatataaaat atatatttaa atttatcttt aatctccttt
    24301 gtaaaatatg taaactttgt aaataaaatc catattatat aaaactaata gttaatctat
    24361 ctaaatacag tttttattta tcacgttttt aaatattttg tgtaaaacct cttttaatat
    24421 aaacacctta ttttcatttc tcatttcata ttctttttaa aatactgaat ataactcttc
    24481 gattttccat ttaatccttt tataaagtta ttattcgtat ttattcctct aattataaca
    24541 ggcaactatc atgtattggt atttgtctat tatataaaaa tctataaaag aaaagcagtc
    24601 aatcatttag attaactgct tgtacatata tcttattaaa ctgtataaac tataattttt
    24661 attattctcc attttcatcc caagtcataa atccccatcc actatctgga taaatatcga
    24721 tagacttttc tctaatcata taaccaactt tataacactc ttttgttatt ttattagata
    24781 gtatacatcc ttctccattt atctaattcc atttaattaa cttttctttt caaccttaac
    24841 ttttataaaa tcatcttttt taataatttc atacttattt tctcttcaac atcatcaaat
    24901 gagggtgtct caaaattaac tttttacttc ataaggcact cttttttgta tgaagtaaaa
    24961 tatattttca tcatttcaac aataagtaag aggcttatct ctattttgag acagccccat
    25021 ttgatggtgt tgaacattta aataaggttg aactcgtttt atctctcttt ttaagatatc
    25081 tgtaaatttg cacataaagt ctcttcttct tttacaattc taatagtttt attaaagcca
    25141 aagtatatat ctccatattc tttcgtatat gatttattgg tactctttca tctactccat
    25201 gaccaccagt tttatacgct aagaacttag gtccaaacgc caccatatct ttaaaccaac
    25261 ttgcatatgt gccaccaccc atagcaattg tatatccatt ttcatctgtt acttcttcat
    25321 aggcttctaa taatttttta agtactaaat tactctcatc cacataatgt aagtctttat
    25381 gaacaataac ctctattttc caaccattac aaaaaacctt tttgaatcta tctattatat
    25441 tgtttaaaac tatattttta ccatacctta tatctaactc acatacgatt ttttcctcat
    25501 ttacttttaa tattcctaaa tttaaggtag tgtttttgaa tacctcatca tattgattaa
    25561 tccccaatgc atctccataa taatcactac aaaggtattt tgaaactaat tctatatctt
    25621 ctctatgctg aaaatccata ttatcctcaa ctatctttat taccatttta gatatagcat
    25681 tttctccctt ttcaggatta cttgcatgta cagctactcc tttagtagtt aactctgaaa
    25741 taattccatt aataattttt gcagtacaat gagatggaac aacattttct ttagttcctc
    25801 cacttactat tatgtaaggt ttacttttgt caattttaat ttctttttct atccttatat
    25861 gaacccttcc tttttctcca ttaaccactg gatacatagc atctggagta aatcccatac
    25921 ttggaatttc ttcaccttta tctaagtaat atttgataca tttcatacct gtctcctcat
    25981 ttgtaccaaa aattaatctt attcttttat taattgtagg atacatatcc tctatgtttt
    26041 tcaatacatg tataagcata gatacagcac ccttattatc tatgacacct cttccataaa
    26101 taatattgtc aattactgct cccgaaaatg ggtctacact ccacccctct cctggtggaa
    26161 ctatatctaa atgcattgga atccctatta attcctctcc ctctccaatt tctgcatatc
    26221 caatataatt atcaatattt ttaacactaa aacctaaact agcacagtaa tctaaagttt
    26281 tatctaaaga ttttttaggt cctataccaa aaggacattg tgaaataggt tcaccttgtt
    26341 gtgaatcaat ttttatcaat tcttttagaa attctatggt tctatttaaa ctattatcta
    26401 cacttctgct tatatccata aatcaaccta ctttctatct ttttaaagcc tgtccttgaa
    26461 gaacaggctt ttagcattaa aaattatttt tttgtaaata ctaattcaca aacttcatca
    26521 tcatgagcac atttttgagc aaaatctaat ttaacacctt gtgcacagtc aactcttcca
    26581 taatccccat aacaagctaa tctacaaatt tcttttcttt cttctttagt taatccatat
    26641 tctttccaag cagcatctaa tggacagtga tggaatttta aaacgccatt ctcatctgta
    26701 ttttctacta attccatttc aaacactttt tgtcctttac tgttatataa catttctgcc
    26761 atttttcctg gagtatctgc tacgttatat ttttgacctc tcattttacc aaatccatat
    26821 attgcttctt tacatatatc atctggatta gctcctaatt tttcagcttc tttcataaga
    26881 agatagaacc atactgctct ttctcctgtt gctcctctaa ttccttctaa atattccttt
    26941 tctgtataca ttttttctga catttctttc tcctcctaaa attaaattct atattttaat
    27001 cactgtgaat tagaatattc caatgaatga ccctaccaat gctaatacaa ctaaaattac
    27061 taataactta gttattgtcc acttcttgta tttcattccc caatatacga agaatacaac
    27121 accaagtggt aataaacctg gagcaatcat atttaatgta tcttgaacaa caattgcttt
    27181 accaccaata tcaaatgata gcggagttga aagcttaact gtagttgcag ataatgctcc
    27241 catcataaac attcctagaa ttgaagcacc atcaattatc ttattgacaa taccacctga
    27301 tagaattttc ttaattgaat ctctacctaa agaatatcct aaatgaaata agtaataacc
    27361 ttcgcaaaat agtaatatac taaacattat tgggaaaaat gcacctataa cacttccttc
    27421 tgctccaaat gaagcaccta aagctaagaa tattgtttga aatgtaccaa aatctaaagt
    27481 atctccaatt ccagccaaag gtcccataag accattctta acacctgaaa taacttctcc
    27541 tgggattttt ccttcagtag ctttctcttc ttccatagct aaaactgtac cgtgaattaa
    27601 tcctccccat atagcttgag tattaaaaaa ttgtaagtgt ctctttaacg catctactaa
    27661 atcttctttt ctcttatata acttttctaa agccggagtg aaacttgcac aaactgctaa
    27721 tgcttgcatt ctttcaaaag agttggataa ttctgctgtc caccaccatc ttagatatgt
    27781 ttttgttaca tctttctttg taagtactct tgtcttatta acttcagttg gaccttgtcc
    27841 atttttaact tctttatttt ttatttcttg cattatactt cacctaccct tttgtcttct
    27901 ttcataaagg taattaaaag agctatacaa acaccaaata ttgcagctgc agtaattgat
    27961 atttcaaaat attgaaccat aaaaaatcct ataataaaca ttggtaaaaa tttattttta
    28021 cctatagtaa atattgttgt tgcaaaacct aatgcaggta atataccgcc cattactgtt
    28081 aatccattca ttaaccattg tggtataaca tctaataatg attgaactaa atctgctcca
    28141 aaaaagttac atacaaaaac tattggaaac cttaaaacaa acccaaatat taaagaataa
    28201 agtgttgcac atctccagat gccttttgta ttaccttctt cagcatactt atctgcctta
    28261 tgaaccaata cagcattacc agttcttctt aagttattaa ctaatactcc tataacacct
    28321 aaaggtaccg caatagatac tgcaacttct gcattaacac cagtttgaag tgctactggt
    28381 attgcaataa gagcagctag acatttatct gatggaatat ttccccctgc tgcgaccatt
    28441 cctagataaa ccatttctat acttgcacca acaatcatac cagttgttac atcaccccaa
    28501 agtaatccaa ggctaaaacc tataaataaa ggagacttta gtgttgagaa aagggtatac
    28561 cctgcaagac ctgctgcaaa ccaataccat aatcctgtta caattgccaa catcaaaaca
    28621 gattccattt ataattcctc ctaatttata ttttttattt aaatttcttt aacgcatttt
    28681 catatgtcat agatgattcc tctggtataa cttgtaggac tatttcaacc ccattttcat
    28741 taagttcatt aagttgttct acatcctcat ttcctaatga aacagctgta aacaccattt
    28801 tctttcctgc ttcatttgga atacctccta attgaatctt ttcatattta agtccagctt
    28861 tataagcctt atttgccata tcaacatttt taaatagaat aaatatattt ttatctccta
    28921 aagtgttgtt ttggaatgct tgtcccgcat cttctgctgt taaaataact acttctactc
    28981 ctgatggtgc tgccatttta tatatgtcag ccataaattc atcttgacct aagaagtcat
    29041 caactattat tattttttga gcttgagcga tttttaccca ttttgttata acttgcccat
    29101 gaattaatct gtggtctact cttactaatg ttatttttgc cattatattc acctcataat
    29161 ttatattttt ttaaattctt taattaaatc cacacaaagg ttattatttt ttttgacgat
    29221 tctttctggt aatgtatgta atttatcttc tttgcttaat tcttcatcta cgattaaaat
    29281 tgtctgtaag ctaagtccat ttattgcatc tattcctctt cttatagcaa cagctattgc
    29341 gacacttgat gtagatcctc catataaatc tgatattata attttcacat cttctttatc
    29401 tactacttct tcaacctctt tcataaatac agaaacatct ttttcagaac ttagtgataa
    29461 aacttcatat tgatttgaca atccaaagtt tttatttgtt tcttctacta agacttttcc
    29521 ccaagttcca tggcacagta tgattacctt atatttattc attgctaaat aaactctcct
    29581 ttatagcgat taatattttg gtttaattct acttcttctt catcaggtac catttgaatt
    29641 gatacattaa tattcttttt aataaattca tctattaaaa gaaactcttt tttattaatg
    29701 tatacatttt gagatatttt cttttttcct attcctgtag gtattcttcc aacaataagt
    29761 tttttgatat taatcccttc ttctatcatt ctatttgcag tatctaaatt tttaattaat
    29821 acaaaaacgt tatcatcagc ttgattatta taaaaatatc tccttgattc acttacagtt
    29881 aatatcgata aatcaatatt ctttggtgct gccattttta tcaaaccttt agcaataaca
    29941 tcattggttg tttcatcatc tactactact atctttttta cacctagatg cttaacccat
    30001 ttatatataa cttgaccatg tattaatcta tcatcacatc tgcaatattt tactaaagac
    30061 atttttaatc aaactccttt ttctattaca ttgagctaag aactttatta atattgttta
    30121 taccagtttg tcctgcttca atacatgctt ctgctaactc ttctaaactt tttccctctc
    30181 tattaattaa agcttcaagt agcattggaa gatttactcc agtaagtgca tgaaacttgc
    30241 cacctttttt aaggtttaaa cttgcaacat tacttggcga acctcctaat aggtcagcta
    30301 atactaaaac tccgtcacct tcatctaatt catttaactt ttctacaaaa tttttattta
    30361 agtcttcaat attatcattc ctatttagtt taattgctgc tgttctttct gctggtcctg
    30421 caataagttc acagcttttt ataatttctt cactaaaact cccatgagtc ataactatta
    30481 ttcctatcac caatttcacc tcacttttct tgtcactttt tatataagca attgccgtgc
    30541 caacttatgt gtatcaataa caaatggcta ttttacagga tttaatcaaa actttttact
    30601 caattttttt taaaaactta ttatttctat atgtatcaaa agattttttc aacctgtatc
    30661 aaatctgtat caaaaaaagt aaaaaaaaga tagccttttg gctattttta aattttgtat
    30721 caacttttga atttatctgt atcatattgt gattcaaata tatctaatat atttgcatat
    30781 tccatatctg aaataattat atcgaatgtc tcttctacta gcttaaaact ttcttttata
    30841 actaaatata attctttatt tctactaatt ctttcttcat atgatttata aactaaagac
    30901 tcttttatta ttactctttc aatcatacat gaacaatgaa atataaactt tactaaaagt
    30961 tcatcttgaa ttactaaaga atagtgttca ctaattctat taattacttc ttttaatata
    31021 aaataagcct tttccccatt taaaaaagtc aaagttttac taatattatc aattagtaat
    31081 ctatcaaaat atctaccacc attatctttt tcagcatatg taacttccat atctccataa
    31141 tgtttcttta tagattctat aatattaaaa tataatttct ctaatgtcat ttctggagtt
    31201 agagcctttc tagttgcttc tattacaata ggtgtactta ccatatctat tgcttttact
    31261 tcttttcctg tagcatcacg aataacttta gcaaatgtta aaatagaccc catatcagtt
    31321 aaaattaaaa ttccttttcc tttatcaatt ttattggcta tttctacagc ttgtactaaa
    31381 atatcctgaa ctctatcttc taatcccata ttaattgcca ctgcatggtt acaatccagt
    31441 aaagtattgg ctacattcac catgctagat gctgtagatt caccatgcat tatgactaat
    31501 attgctatac tctcctcatt taatcccaca tacgataaat aaagaaatgt tgctaaaaaa
    31561 attgcttcat cttctggaaa ctctactcca tacattaatg acatgttaga taatatttct
    31621 tttgctatgt tatattcata tgctctttcc ttcataattt catctttatt aggatgcttt
    31681 actatatttc caatctttat tctttcaatt aaattctgaa tatgtaaagc tatagctttt
    31741 attaatttat ctaatccttc tcttttgctc catttatcat gcttttgtaa aatatatttt
    31801 acagtagttg caattgaatc attaacaatg ttgttataag ctgtttgatt actactatta
    31861 tatataaaag tattcattaa attataggaa tacttctgta tgtctccatc tataacactt
    31921 ctaatttgtg actctttaat accttcttgt tgaagtttat tccaagtttc ttttatcatt
    31981 ccataaaaat ctaaatcata ttttttattt tctattaata aattttcact ttcaaaatcc
    32041 ttttcttctg gtaaaaaaat aatatcatct tttccaataa aactgaaatt ttgtacaaac
    32101 tcctcttttc tattatttcc atataatgat tctctaatat tatttggtag aagagagagc
    32161 cttacatata cacattccct tttataggtc atataatcta aaaaagctct tgcacaaatt
    32221 aattgtatat cgctttttaa ttggcctatg tttcccttac acttatataa aataaatgcc
    32281 ttaataactt cttttgaaac ctttaactta acaccaatcc taacactctc ttctttaaaa
    32341 aaataacata taagcttcat tctctcttga aagcctcttt cctctaaaga cggaatcttt
    32401 atagttacag gtattcttct taaaaacgtt tccaacattg ctgtttctgg gtcttctgta
    32461 gtggctgcaa ttatcataac tctacactcg tgtaccttat tagcctctcc taaccttcta
    32521 tatatccctt tatccataag cagaaatagc atttcttgtc cctctgcaga taatctatga
    32581 acttcatcta aaaacaatac tccattatta gcttcatgta caataccata cttatcttta
    32641 tcagctccag taaatgctcc ttttacatat ccaaaaagct gcgctaataa taattgctga
    32701 ttatcagaat aatcagcaca gttaaatatg ataaatggtg cattatcact atcttctttt
    32761 atagttaaac tatacttata catatattct gcaaatatag atttaccaac tcctgtaggt
    32821 cctattatta atgtactcaa ccccttaggt ggatatatta ctgctgcttt agcctgttct
    32881 atttgggacc ttaaacttcc atcacatcct actaaattaa aaatattgtt ctcttttttt
    32941 atactctcta aaatatactc atctttgata gaatcatttt cactatattc gtttatagag
    33001 ttaatattat caaatatccc tttatttagt ttctttccta aaatactctc aatactttct
    33061 ttatataagt actgaacagg cttacccttt atctttataa taatactttc ttttattaat
    33121 tgatttaatt ccttactagc attacttcta tttataccta cttcagtagc tatatattct
    33181 gcatcaaatc caaatctaat gccattatta aagttcaagt gttcttctgt taaccttttc
    33241 aagcaaatta aaactttttc tttccttgat agcttctttt gtgtcatagt taacctcccc
    33301 tataagcagt aatactgtta aacatttatt attaagctta attctacaat attttaatat
    33361 ttttgtctaa ttatgtaact attgattttc tttcctattc gttttataat ataaattacg
    33421 ttaccctcat cttttataga ggaggaattt atatgaattt tttatcagat ttacacaccc
    33481 actctatagt cagtggtcat ggctatagca ctttactaga aaatattaat tattgtaaag
    33541 aaaatggaat taagatacta ggtacatctg aacatggtcc taagatgcct ggttctcccc
    33601 acagatggta ctttcacaac attcaaaata tcccaagaat cataaataac attataattt
    33661 taagaggctg tgaagcaaat atactagata ttaaaggaaa catagaccta gagccttttg
    33721 taattcctcg cttagattat cttattttgt catttcacga agctgtgttt tctccaaata
    33781 cattagagaa taatactaaa gctttaataa atgcaataaa taagcatgat aatattgaaa
    33841 tattaggaca ccttggaaac ccaaactacc caatcgacta tgaattaatc attaaattag
    33901 ctatagaaaa gaatatctta atagaaataa ataattgttc tattaaaggc gtgtcaagaa
    33961 atggtagctc ggataactgt aagtatattg ctacactttg caaaaaatat ggtgctaaaa
    34021 tcatactgac ttcagatgct cacatttgtt ttgacatagg taactatgag tattctgaaa
    34081 atatcttaaa ggaaattaat tttccagatg aattaataat gaattatcct aaaaaactta
    34141 ttaatcattt tcaccaaaaa ggtaaattac tagatgttga ttatacaaat atctaagtaa
    34201 taatacatgc ttactttaac tcccataaaa attttaatac tatttatatt atatgaaata
    34261 aagaaattat aataaagaag ctatttaata aagcttaatt acttttatga acttgatgga
    34321 ttgatatggt ctatgatagc aatagaaatt ataacatttt taataaactt agttttatat
    34381 accttatagt ataaatctaa aaaagcatca tctaaaaagg agctgtcaca tgagcaaatt
    34441 aaatttgttc tatgatagct ctttttttac ttattttaat taggtatatt tttaattatt
    34501 ttatataaag cttatgttct aagtaaaatt atgcataaca aacaaaggta taaaaatacc
    34561 attttttcag ataatattta attataaata ataaatttag aatacttctt ttaaatgatg
    34621 agagtctaga aatttttatg tgtattcttt aaataaaaat ttccagactc tagccccttt
    34681 ttgaatattt taaaatttaa cttaaactaa gcaaattcta taatag
//
LOCUS       DAEMGG010000028        34631 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEMGG010000028 DAEMGG010000000
VERSION     DAEMGG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.028"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(278..1879)
                     /locus_tag="KPK24_001357"
     CDS             complement(278..1879)
                     /locus_tag="KPK24_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2299042.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTAINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGINGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1990..2667)
                     /locus_tag="KPK24_001358"
     CDS             complement(1990..2667)
                     /locus_tag="KPK24_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299043.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2680..3375)
                     /locus_tag="KPK24_001359"
     CDS             complement(2680..3375)
                     /locus_tag="KPK24_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG2299044.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3427..4626)
                     /locus_tag="KPK24_001360"
     CDS             complement(3427..4626)
                     /locus_tag="KPK24_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG2299045.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4887..6932)
                     /locus_tag="KPK24_001361"
     CDS             complement(4887..6932)
                     /locus_tag="KPK24_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2299046.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKYFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7343..8152)
                     /locus_tag="KPK24_001362"
     CDS             complement(7343..8152)
                     /locus_tag="KPK24_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2299047.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8236..8769)
                     /locus_tag="KPK24_001363"
     CDS             complement(8236..8769)
                     /locus_tag="KPK24_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2299048.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8919..10268)
                     /locus_tag="KPK24_001364"
     CDS             complement(8919..10268)
                     /locus_tag="KPK24_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG2299049.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(10280..11662)
                     /locus_tag="KPK24_001365"
     CDS             complement(10280..11662)
                     /locus_tag="KPK24_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG2299050.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRVRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            11906..13120
                     /locus_tag="KPK24_001366"
     CDS             11906..13120
                     /locus_tag="KPK24_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG2299051.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            13297..15210
                     /locus_tag="KPK24_001367"
     CDS             13297..15210
                     /locus_tag="KPK24_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2299052.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYKNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            15481..16455
                     /locus_tag="KPK24_001368"
     CDS             15481..16455
                     /locus_tag="KPK24_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG2299053.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            16476..16751
                     /locus_tag="KPK24_001369"
     CDS             16476..16751
                     /locus_tag="KPK24_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2299054.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            16764..17927
                     /locus_tag="KPK24_001370"
     CDS             16764..17927
                     /locus_tag="KPK24_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2299055.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGSAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKTESLGHREFGIYRISSTF"
     gene            18264..20543
                     /locus_tag="KPK24_001371"
     CDS             18264..20543
                     /locus_tag="KPK24_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2299056.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVVAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVMNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(20638..21978)
                     /locus_tag="KPK24_001372"
     CDS             complement(20638..21978)
                     /locus_tag="KPK24_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2299057.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(21990..22772)
                     /locus_tag="KPK24_001373"
     CDS             complement(21990..22772)
                     /locus_tag="KPK24_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299058.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(22787..23809)
                     /locus_tag="KPK24_001374"
     CDS             complement(22787..23809)
                     /locus_tag="KPK24_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG2299059.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(23815..24828)
                     /locus_tag="KPK24_001375"
     CDS             complement(23815..24828)
                     /locus_tag="KPK24_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG2299060.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTKFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(24956..25894)
                     /locus_tag="KPK24_001376"
     CDS             complement(24956..25894)
                     /locus_tag="KPK24_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2299061.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEDKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            26164..26628
                     /locus_tag="KPK24_001377"
     CDS             26164..26628
                     /locus_tag="KPK24_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299062.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILDGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(26740..27057)
                     /locus_tag="KPK24_001378"
     CDS             complement(26740..27057)
                     /locus_tag="KPK24_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2299063.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(27070..27801)
                     /gene="chbG"
                     /locus_tag="KPK24_001379"
     CDS             complement(27070..27801)
                     /gene="chbG"
                     /locus_tag="KPK24_001379"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG2299064.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(27827..29137)
                     /locus_tag="KPK24_001380"
     CDS             complement(27827..29137)
                     /locus_tag="KPK24_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2299065.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(29234..30547)
                     /locus_tag="KPK24_001381"
     CDS             complement(29234..30547)
                     /locus_tag="KPK24_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2299066.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(30569..30880)
                     /locus_tag="KPK24_001382"
     CDS             complement(30569..30880)
                     /locus_tag="KPK24_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2299067.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(31080..31784)
                     /locus_tag="KPK24_001383"
     CDS             complement(31080..31784)
                     /locus_tag="KPK24_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2299068.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCEYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(31875..33236)
                     /locus_tag="KPK24_001384"
     CDS             complement(31875..33236)
                     /locus_tag="KPK24_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2299069.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            33735..>34631
                     /locus_tag="KPK24_001385"
     CDS             33735..>34631
                     /locus_tag="KPK24_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2299070.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKK"
BASE COUNT        11932 a         5854 c         4215 g        12630 t
ORIGIN      
        1 ggggttattt agttatcatt ttttatttct aaaaatcttt tctcaaatga tttagttatc
       61 tatttatcca attttttaca taatttaatg attaaaaata ttaatttttt cactataaag
      121 tataaatatt ttttgtataa atattatctg attattaaaa taaatattaa tactataaat
      181 aaaaacgaag catgattttt atttaaataa atctcaaact tcgtctttac ttacttagat
      241 atttatttac tttctaaatc ctattatttt taaaacctta tctttttaat acttcaccac
      301 atttattctc agtaatctct ccattttcca acgcaatttt tccatttaca attacatatt
      361 caacaccatt ggctagtaat tgtggctttg aaaaatcaat atcttctgac ctagaataac
      421 tatagttatc catgtcaata actgctatat cagctatcaa cccttcttta agaatacctc
      481 tattttttat tttaaattgc ctagctggta atgaagtaac tcttcttaca atctcttcca
      541 atctaacacc agactttctg tacatattga agaaaattgg aaaagctcct cttctttgta
      601 gttcaaacca tgacatatcc ccacccacac taaacaaaca atctgaacca accataacaa
      661 atggattatc aattatttta tcatcatgta gtttatctgg gaaatcagct ctatacattc
      721 ctcctaaaca aaagataagt tcttcgtctc catctaaaag taaatcgagt aataacttgt
      781 cttcatctat tcctttttgt gtagctattt cacgtattga tttcttttcc atattaatat
      841 ctttagtgtt tagaagtatt atactccccc tatctccaag tataaatgct tcttttaata
      901 taagtgttct tccctcttca tttctaagag catccattac cccatttata cctaattcaa
      961 atagactaga tgatattgcc ataataaatt gaattactct ttttttcttc atacaatgcc
     1021 cagaacttct tggtactgtg tcaaccatta tttcaacacc attataccta gcctttaata
     1081 tctcatcaaa tgcttctatt tcaactggac ttaagtgaga tacttgaacc ctagcaccac
     1141 ttttttgccc aacctctatg gcttctttta ctgcattata tcttcctatg taatctctaa
     1201 tatgagatgt atatatacca tcatactctt taatctgcct tgcaatatca acaagttcat
     1261 caatatcagc atacttactt ggtatatatg ccaatccagt agatattcca aaagcaccac
     1321 ttttcattcc atcatttatt atatctgtaa tttcattttt ttccttttct gtaggaggtc
     1381 tatccttaga acctcccata acactccatc ttatagtccc atgccccaac aagaatccca
     1441 tattgacaga aataccactt ttagatataa ggtcacaata ttctgtaaaa ttattccaac
     1501 atttattttt atctataaga tacttttttt cttcttcaag taatagacca tttttataca
     1561 tatattcata cacattttca gaagaatatg gtgttataga atgtccacaa ttaccattta
     1621 tagctgttgt aactccttgt tttagaaact tttcaaattt accatctagt attgctacta
     1681 tttcttcatg aacatgaggg tcaataaatc caggtgttat aacttttcca caacaatcaa
     1741 tctctttatt tgtattgcta tttatcttag aatctatttt tgctattaag ttgtctttaa
     1801 ttgctatatc tccataaaaa ccaggattac ctgtaccatc tattataaat ccatttttta
     1861 agattaaatc atatatcaaa atattccctc cttaaaaacc aatttttaaa taatcttaat
     1921 gtaattttta tatttgaaaa tcaaattatt aaatttcata tttgaaaatt cactattatt
     1981 tcattgtaat tagtcactaa gcttatcttt atacatagct cttgcaatta atatagtacc
     2041 aaatacacat ataagaacta ctagagactg tggtattata ccttgctttg ataatataat
     2101 agtcaataca gatattacaa ttgctactag acctaatttt ttatcttgta aaaatacttg
     2161 tccaaaaact gacccaaata gagctggtaa aattaagttt aatttttcta ctacttcagc
     2221 tggaatttta gatagtacgc ttcctcctaa aattacccct atagttagta tacttatatt
     2281 cactaaaata gatacagcta ttcctattgt agatattatc gagccttctt ctgtacctga
     2341 ttcaacttct gctgcctttt gagctgctat tgaacatggt agcctcatat tagatatatt
     2401 accagaaaga aaactcatat aagttcctgg tatacctaag attggtgaat aagaaactgg
     2461 ttctattatg taatttggag cactcatact gacaattgcc attgtacttg ctaataatgc
     2521 tgatattgga ggcataatac caaatccaaa gctcaaaatt attccaggaa ctaatgctgc
     2581 tataataccc aaagatagtg ttagtcttcc ataccttatc ataaaaggta aatattcttc
     2641 attgtatata gattttactt tactcataaa actcccccct tatattactg aagctattat
     2701 cattccgcta aacattgata ttgtaagccc ccactctctt aaccatttta tatttttctt
     2761 cttttcaagc atacacagta ttaccattat agccattcca cttatacatg caattgttcc
     2821 attatcaaaa cgcaacaccc tatcagcatt tagatatgca aatgaaccca acattgctcc
     2881 tagagatatt attggtataa atgatttctt gccactagat ataacattgg ttatcttatc
     2941 tagtttatgt ccaaatagta atgtgaatat tatccatcct aaagaaccta atatcatagt
     3001 ccaaactgca tttgtaaatg caagttttgt catttcagga gaacctaaag ttatacctaa
     3061 tgcatctgtt ccaaatccgg ctgacattga ttcaaaaatt acagaaccta taaatgataa
     3121 tctcatccat gctataggcc cacccatagt tactataagc gataccattc cagcaaatat
     3181 agtcattgaa ggcccaatag ctgaaatagc actactctta acagctgact ttatttgttt
     3241 atctgttatt cccattgttt taccaacttt gcaagatttt atagcaaata taagggattg
     3301 taccactact attgatactg ctactgtact cgctatccac aaaagtggat gattagctaa
     3361 ttttaaataa tccatttata aaacctccct ataaatcctc aaattttatt aatatttcta
     3421 atatatctat ttccaaacct gttttagtac tctaagtata tttccaccta gtaccttctc
     3481 tatttcctca tcactatatc catgctttac taaatatcta acaatgtttt ttgaagtttc
     3541 tgttgggttc tcaagacctt ttacatattc tactttggga tgttcttttg ttcctgttat
     3601 tttttttata tcaaatttag cagctagtac tgagtgtaat tttacatggt cgccatatac
     3661 tgtatctggt ccaaaagcta catggtctat tcctactaaa tttttaatat actcaaaatg
     3721 ttccataaat gactctattg aatgttcttt attcttctct gttattgtag tatgaggtgc
     3781 tgcttctaca cctataaccc ctcctttttt agcacatgct attaatactt catctggagt
     3841 tagtctcttt gtgttccata aatttcttgc cccaacatgg cttaatatta ttgggtcctt
     3901 gctatattct attacatcaa gtgtagtttg aactccaacg tgagagcaat ctattgccat
     3961 tccaatttta ttcattcttt caacagcttt tttcccaaaa tttgtaagac ctccatcttt
     4021 ctcttctttt agtcctgaac ctaatgcatt ggattcgctg tatgttatac ccattaacct
     4081 tataccaaat ccatacagta tatctattct atcaagttca ttttctattg gtgctgcacc
     4141 ttctaatgtt ggtatcaatg ctactttacc ttcactttta gctctgtata tatcttctac
     4201 tttttcacac tttatgacaa aatcttgatg agctaaatca cataatctca tgcctaaatc
     4261 tattaatata tcatcccatt tccaccctga atttgatgtt atcgtacata caccatccat
     4321 taaattatca aatatagcat cataatatcc tcttgataat gcttcataag cacatctttg
     4381 tttaccttcc ctattatatt caaatatatc tctttctagg tgttctggaa ataatactgg
     4441 atgttcatgt aatgatataa atattttttc atccactata cttttaacct tagcttcttg
     4501 aatatcatct aattcaataa ggtattcttt aactctttta tctcctttac acatctcaat
     4561 agatgaaata tcttttcctt tttctaaata actataggct tcatatcctt tgtaagctga
     4621 cttcataaca acatcctcca agttctacaa ttttttatat ttttcctatg tagcaattga
     4681 tgtgccaaaa tataaatttt aattgtataa taaatcttgc aaaaaataaa aacagtagta
     4741 aggttgatat ttactactgt tttattaaaa ataatcttct tagattattt attcaatatt
     4801 tttgaattat cttaatctta aagataattt tatttatttc tatccattat agggattttt
     4861 ttagggtgtt ttgccttttt agtcccctat atcactccct taatttttac atatgatata
     4921 aagttctttc cttcatcagt tataaaacta ccttgttttt ttactccaga taaaagtaaa
     4981 ccatggctct ttaacttatc tattcttttt ctaatttggt ctacacttaa aactatatct
     5041 ttttctttta aaatctcttg cagtttattt cttcctaata atatattttt attattccaa
     5101 gtttctatac tggtcaatat acatatactt tcctcaaaga aattagattg tttgaaatct
     5161 aagataattg aatcgaaatt ttcattaact aaagtattgt ttttttcaaa tctaaattgt
     5221 tctggcaaat gctcgtaatc tactctatct tcctcaacta tagtatcaat ataataaatt
     5281 aaatttttaa gctctcttac atttcctggc cattcataca atgttaaagc ttgcattgat
     5341 tcctctgtaa aatatttgtt gctatttatt tcatctaaaa aatacttact tataagaggt
     5401 atatcttcct tacgttctct aagtctaggt atctctatat gtaatacatt aatcctataa
     5461 tacaaatctt ctctaaaaga accttcttta atttttttct ttaaatcttt atttgttgca
     5521 gctataattc taacatctat tggtataatt ttacttccgc ccattctcat aacttctttt
     5581 tcctgtaata ctcttagcaa tctctgctgt acatctaaag atatatcacc tatctcatct
     5641 aaaaaaattg ttcctgtatg tgctctttca aatataccca tttttccacc ttttattgct
     5701 cctgtaaaac ttccttcatc atagccaaaa agttcacttt caataagtgt atctgacaaa
     5761 gaagatagat taactgcaac aaatggcttg ttttttctta gagattcatt atgtattgcc
     5821 tgggcaaata tttcttttcc tgttccattt tctccaagta tcaacacaga aaagtctgta
     5881 gttgctattt ttcttgcaat attaattttt tcttttatta ttttactctc acctacaaca
     5941 ctctcaaatg tatactttga tacaaaacct tttgactgaa gtttattttg aatcttgtcc
     6001 tcaagcactt gaattgcact tatattttta atgcttataa tactctttat tcgttcatta
     6061 ttttcataaa catttacttt attaattatc agttttttgt tatttaaatt tactacttca
     6121 tcattaactt catcttcttg aaaaaatatt tttttgacaa ccttatcact aaataagtcc
     6181 ataaagttat tagatataat ttcattttgg tctattccaa ctagattaga aaatactttg
     6241 ttgcaatata taaattttcc taatttatca acagaagcaa taccttcatc tataatctcc
     6301 aaaaaactct tcatggtttt attcatagtt gtataatacc tatatccact tactgtatct
     6361 tcattgtatt tttccttaat aatatgtaat ttatctatag atatatttag ttttgtaaag
     6421 atttctataa ctgtatttat atcaattacc ttatcaccta tgtcaattat ttgctttatg
     6481 ttttgtggta tgctgtttct gcttcccgta ataattccaa tctcacattt agacttatca
     6541 caacctggat aatatggtat tagattaata tgaattaatc ctagttttct aattatcttg
     6601 gcagtttcat ctgctgactc tttataagca tctataacca taacatcaga atcattttct
     6661 atacttatta tctgatttat attttcaata tttatagtac gatgtactat tacaataggt
     6721 atatttttat caatattatt atgtatatat tcctttatat cattgtctga acatactaca
     6781 agctcatagc ttgacagttc aatatttatc ctatttacat ataccaagtt atctatatta
     6841 caaaattctc caaatatatt ttccaattgt tttgatatat ctatatttat tttgtcatca
     6901 gttgatacaa ataaaatatt atgttttttc atagtcccac ctcctgttta acgagtatag
     6961 aaagtaataa tttggtctat tgtagcactt tttaaaaaag ttttaaatct taaaaatcaa
     7021 attttgtacc atttactatt attttccata ataaaataat gtaagctatc ttttttcaca
     7081 tataatttaa tttctaaaac ggttagtttg acattattgg aatataaaca aaaatttaat
     7141 ataaattcgt taatatatta atacattttt aagaatgaca aggaataaat tataacaaat
     7201 tttaggaata tataataata gggtcttaga acttagttac aagttctaaa accctatttg
     7261 tttagtgtaa aaaacatcat aaaatttagc ttaaatcttt tcacataata ttactctttt
     7321 tctaagtgat aataaaatat atctattcct ctaaaaactg taaaaattcc tgattaaata
     7381 gtttaagctc atcatagaat atagcatgac cactatactc aaaagtatat aaaattgaat
     7441 cttctatttt ttcattcata aaaactgcaa attcatacgg acatatttca tctaatttcc
     7501 catgaaatat accagtaggc actcttacac attttaaatc attaaataat tcttcatctc
     7561 ttagagatac agcagttcca atagtgccaa tacctgatgc actaaaacca atatcattaa
     7621 accatcttct aaaactttct ggtggatttg atgcaaatac tttttcgcca aaatcggtta
     7681 ccatttctgg tctatcagta catgcttgtg ctatcaattt atttacatct tcacgtgtca
     7741 taccatatgg aaattctgga ggtctttgta caaatgatgg tgcagcagct gcagctaata
     7801 ccaattttga cactccatat ccattaaata aactcatata cctaagtacg attgctcctc
     7861 ccattgaaaa accaacaagg gtaaagtctt ttaatccaat agcatgtacc accttataaa
     7921 tatcgtctgc taattggcta taagtatatc ctcctgatgt tgcatcagat ttaccaaaac
     7981 ctcttaaatc aattgaaact gttctatatc cgagttttgg caatatattt gtttgatatt
     8041 caaatatctt atgtcctaag ggccaaccat gtataaaaag tacagttttt ttagcactcg
     8101 gattcaaatc atatactgct atgtttatat cattcacata tacataatac atatcaacag
     8161 caactccctt caagaaaatg tatgatggtt attttatata tatgaataaa atgatgaata
     8221 actaaatttt caaatttatt tacaatactt ctctattgcc ttttcaaata aacttacatg
     8281 aagtttttca tctaggatta ttctatttaa tatagctttt atataagggt cttcaataag
     8341 acttatatct ttataataat tattaatagc cacatgctcc aaatgcaaat ctagttttaa
     8401 tctgttacaa atagaatttc cataacatac aaaaccacca ttccaatatg caccacaaga
     8461 acttgtagaa cctctatata caggatttcc acccaataat cttatagtct ttgctaatat
     8521 ctccatatgt aacatttctg ttatagatat atttatccac atttcactta actctctatg
     8581 cgtttcatct aaatcaaaat tatggtatag atattgattt acactcgtaa attcacttga
     8641 tactccggca tagtcatcca tcaataattc tgcatagtac ttatttggac ctaaaacttt
     8701 tatctctgga tatggctcat cacttgaata tccatttcgc ttgtgcatgt cataatgtgc
     8761 atcactcata atatctcctc ctttaaattt aatgtatgat attaattgta tgcatcacaa
     8821 ctttttcata taacttagat aataaaatct acttaaataa taaaaagcca tattaattaa
     8881 aattaacatg accttttatc cacaaaatta ctttttaatt attcatcttt tccagctact
     8941 gatagctctt taataacaac tgaaggtgaa cctatactac tcattggaaa ttctaaatct
     9001 tctcctatag cttcaatatc tttcaacagg tcaaaatagt tccctgctac tgtaatttgc
     9061 tccactggaa atactttttt tccttctttg atataaaagc ctttggcagc taaagaaaag
     9121 tctcctgtta ctgaatttgc acctgaatga agtcctgcaa agctagtaac catcaatcct
     9181 tcatctatat cctttataat ttcatctaaa gatttatctc ctttctctat ataaaaatta
     9241 gttggatata tgcttatagg tgatgaatat gaagatttta ttccatttcc tgtagtcttt
     9301 acacctgcct tattagccgt ttttaagtta tgaagtaaag ttataagctt tccatttaaa
     9361 acaacttctt ttttaaatgt agctactcct tcatcatcaa aaggagtaga tgccatacca
     9421 ttttctaaaa gtgggtcatc aactattgta actataggag atgctatcat gtctccttct
     9481 ctatttttca ataaagataa tcctttttga gctgaatctg cattaaatat cccagaaaat
     9541 gtgctaagca aagataccat agcttcatta agcaatatag ttttatattt acctgatgga
     9601 atactcttac cacctacttt agataaagca ttatcaacac caagttttgc tatctcacaa
     9661 ggttttattt cttcaataga atttgctata ttatatccta ttccatcata tttttgtcca
     9721 ttgtcttcta tgataggaac tacaaaccct ataagcatat tagttttatt gcttaaatta
     9781 agacctttag tattagatat accattactt gatacagaat aagatatagt acatttagat
     9841 aaatttacca ccttatctga ataagcttta gtctctcttt ccatttctaa agctaaatca
     9901 atcaacttat ctgcctctaa attttctagc tctttagaat aagtctttac atcattatag
     9961 tgcttatctc cttcatatat aaattgtaca tcttcatttt caatagtgtt tactccatct
    10021 tttacatttt ttatcaacat atcaacagct ttatcatcta aaatttctgt ataagaataa
    10081 cctatctttc catttacctt acctctaaaa gataaaccaa aagatttatc aagattgtat
    10141 ttctcaactt ctccttcata tatatttata cttaaatttt ctcctgtata ataatatatt
    10201 tcacactctt caaaaccttc acttagagcc tttttaaata atatatcttt aaaattttta
    10261 agttccacaa ttatccctcc tatcttccac ctacagtaat ttcttttacc cttatcatag
    10321 gttggcctac attagttggt atacttcctg atgatgagcc acacatcccc tgagcttgtt
    10381 ttacgttatt cccaaccata tctatattca taagtatatc gctacctttt ccaattaaac
    10441 tagcacctct aactggctct tgtatttctc cattttttat taagtaacct tctgaaactg
    10501 caaaattaaa ctctcctgtt acaggattta cagaaccacc acccattttt ttagcataaa
    10561 gtccatctgg tattgacttt ataatatctt ctggtttatc ctctccagca gcaatataag
    10621 tgttagtcat ccttgaagtt ggtgcaaatt tgtagttctg tctacgagaa cttcctgtag
    10681 gattcattcc cattcgtcta ccattaagtt tatctatcat ataagacttc aaaattccat
    10741 tttcaatcag tatattcttt tgagttggat ttccttcatc atctatattc atagaacccc
    10801 agtagtttgg tattgtacca tcatctatag ctgtaatctt tgtggaagca atctgttgac
    10861 ctaatttatc tgcaaataca gagttcccct ttgcaactga tgtagcttcc aaagaatgtc
    10921 cacaagcttc atgaaatatt actccaccaa atccattatc tattgctacc atcatattac
    10981 ctgcaggaca atttttagca tgcaacatag tatgcgctac tctagctgat tcttttgcat
    11041 aatattctgg gtctatttct tcaaatattt caaacccttt acaaccccca ggaccttcaa
    11101 atccagtttg attttcatta cccttagatg caattgaact tatccccagt ctggttctta
    11161 ctcttctatc ctcaacatat ataccttcag tgtttgcaat taatattttt tggtctttat
    11221 ccaaatagct aacacttact tgagatatat cattactaaa ttcttttgca ctcttatatg
    11281 caaccttcat aacacttact ttatctctat ttcctattga atttggataa atttttatat
    11341 tatgtatgtt atttatttta atactgtcat ttaatattac actttttcca tcttccaatt
    11401 tacctaaagc aacagcagct ttgtatgcta catccaacag actatttaat gaattgtcac
    11461 ttgtataagc atatatactt ttcaatcctt taaatattct tattcctata ccataatttc
    11521 ttccacctat tgcattttct actttattat ctattaaact aatagaatta tttatagtgt
    11581 cttcttcaaa aatctcagca aaatctcctc cagtaattag acacttttct aataccttag
    11641 aagctatatt ttttgaaagc ataaatttac ctcctacttt aaatttttaa ttaactttta
    11701 ttcaatcttt ttacttgtta acaaacatta tatactatat tacttaaaat aaggtaatta
    11761 tgaatatatt atacatatgt tttatactaa aatgttatca cattttatct atacataccc
    11821 atataactag ataattaaaa tgtataatat tatttgctat agaaaataat acactcatac
    11881 ataataaatt ttaggagtga agtatatgaa tcaagaatat gtaaaaggaa atcctatttt
    11941 tactaatatt aataaagttc caagacaatt tccatacctt actgatgata ttgatactga
    12001 tgttataatt gttggtggtg gcgtaactgg atgtatttgt gcttactatc tagctaaaaa
    12061 caatataaag tctgtcattc ttgaaaaagg aaggattgca catggaagca ctagcgtgac
    12121 aacttctcta ttacagtatg aattagatga caatttgata gatttaacag aggttatgac
    12181 tctaaaagat gctctaaaag catataacct ttgtacttca gcacttgaag aattagatac
    12241 atttatagag ctatatggaa ataagtgtga ttatgccaaa agagatactc tactttatac
    12301 tgccaacaaa cttgaagtta aggctatcaa agaagaatat aacttaagaa aagaaaatgg
    12361 atttgatgtt gaatatattg atgaatccac aaaccctttt agctttgatt taaaatcagg
    12421 attgatagca aagaatggag gacgagaatt agacccatat aaatatagtc accatttgat
    12481 tgatgtaagt ttaaaaaatg gtcttcaagt atatgaaaat acagaagtaa aaaaagtaga
    12541 tttatcaaat gataaggtta ctgctgaagt atcgtatgga tacaaagttc atggtaaaaa
    12601 attaattgta gctacagggt acaatacaag tttatttaca aaaagaaatt ttgcaactaa
    12661 gtcaaatact tttaatatag caacgaaacc acttaaaaac atagcttctt ggaaaaacaa
    12721 cattttaatt agagataact gtgacccata taattatcta agaactacta aagacaatcg
    12781 attaattata ggtggtgaag atgtaagttt tgatgatata gaaaatgaaa ctttagccaa
    12841 tgaaaagtac gatattttag aacaaagatt gaaaagtatg tttaaggata taaaagacat
    12901 tgaagttgaa tataaatatt gtggttgttt tgcttcaact ttagataatt taggttttat
    12961 tggtccagat aataaaaata acaatctatg gtattgctta ggttatggag caaatggtat
    13021 actctttgca atattaggag gtataatgtt aagtgaatta tatcttggca aacaaaataa
    13081 aaatatgaaa ttatttaaag tagatagatt tgataaatag atattatata ttaaaagact
    13141 gtaaaaagat gaaactttat aattctaaaa gttaactttg atacagtctt ttataatgtt
    13201 ccttttaaaa atactttatt tataattaaa tataaagtaa aatgttaaaa tataactgat
    13261 aaaatttagt cataagataa tatttaagtt tatttgatga aatatggagg aattattatg
    13321 aaggaaatac tcgttattgc tcccactaaa ggaacctatg aaaaatcaat tcacatagtt
    13381 aaaaaaaaca aatataaaaa tattgatgtt gtatttggca atctaaaaga aggtatacct
    13441 cttgctgaaa aaagtattaa tcatggtact agaattatca taagccgagg tggaacttac
    13501 aatatgttaa aagctactta caatatacct atagttgaaa ttaaagtaga tgcttatgat
    13561 attataaaaa gctacaaaga ggtaaaaaat tccaacgaac catttggaat aattggattt
    13621 aataatgtca tctacggttt cgatattata gaagaaatat tgaataagaa aataactatg
    13681 attgaaattg aaaaagaaga agaaatttat gatgctatag aaaagtacag aaagaaaggt
    13741 ataaacacat atataggaga taccactgtt gcacatattg ttaagagact taattgtaag
    13801 ggaatattaa tcgaatctag agaagaaaac atacttagag ccattcaaca agcagaacaa
    13861 atactcgaag caaccaagga tgaacaaaaa agaagattac agatagaagc tatgacagat
    13921 tttgtacatg atggaataat aacagttgat aaaaatttta aaataacatt atttaataag
    13981 agtgctgaaa aaatatttgg tataaaaaaa gaaaatgcac tttatcataa tgttgtagat
    14041 gttatcccaa acacagttct tccacaagta gttgagacta tggaacctca aattggtgaa
    14101 atacaaaata tagggagtac aaaaattatt acaaatagag ttccaatcat tgttgatggt
    14161 cagattcaag gagctgtttc aacatttcaa gatagcaagg agataagttc ttttgaacat
    14221 acaataagaa ggagcttatt aagcaaaggt ctatctgcta aatacacttt tgatgatata
    14281 attcatgcta gtgaaaagac aaaagattgt atagaacttg caaaaaaata tgcttgttat
    14341 gatactccta tgattattac tggagaatct ggagttggta aagaactatt ttgtcaaagt
    14401 atacataact atagtaagcg taaaaatgca ccttttgttg ctgtaaattg tgctgccata
    14461 cctccatctc taatagagag tgagtttttt ggttatgagg aaggttcatt tacaggtgct
    14521 agaaaaaaag gtaaagcagg tgtattcgaa ctagcacatg aaggtacaat cttcctagat
    14581 gaaataagtg aaatccctct tgaacttcaa ggtcgattat tgagggtatt gcaggaaaaa
    14641 caagttatga gaataggtgg tgataaagtt attcctatag atgtaaaaat tatctgtgct
    14701 tcaaataaag acttaaaaaa tatgatgcgc aatggtttat ttagacaaga cctttatttt
    14761 agaataaata tactaacatt atatcttcct tcattaaaag aaagaaaaga agatattata
    14821 aaattatcag aattttttat acataaatat tcaagcaaat acaataaaac gcctttaatt
    14881 atcactccaa atattaaaaa ggcacttctt gaacgagaat atgaaggtaa tataagggaa
    14941 ttggaaggta tgatggaaag ggctgttatt gtggaatcat ttgactcgat tttattagaa
    15001 aaggttagca atgaatatac aaataatata gttgataaag ataatagtga ttttgaatgc
    15061 agtaatttag atttaaaaac tttagaaaaa aaatatatct ataaaattta taacgaaaat
    15121 aatagaaata ctacaaaaac ctgtgaaatt ctaaagataa ctcgctccac tctatggcgt
    15181 aagttaaaag aaatagaaga tgatgtttaa aaataataca tacctatata tatcaaaacg
    15241 ttttatgttt aattttaata cataagacgt ttatttgttt tcaataaaaa atacaattat
    15301 atgataaaaa gaatctttgt aagtgaaatt ctcgtcaatc gtacttttaa ttataagtaa
    15361 tctacagcat ttcaatcgat tattaaaaat aatcatgata actaaagata caaactctat
    15421 atattggcat attaattgct tttataatac ctatataaaa atgaaaggga gtgatactag
    15481 atgcacatct taaaatcagt gaaaaagata ccaggaggac ttatggtctg tcccttgtta
    15541 ttgggagcac tatttaatac agtatgtcca caagctcttg aaattggagg attcacaaca
    15601 tccttattta aaactggtgc aatgagtata cttgctctat tttgtctatg caatggagct
    15661 caaatcaata tcagacaagc tggtaaacca ttgactaaag gtatagtact tacagcaaca
    15721 aaatttataa ttggtgcagt attaggtatt attgttagta aatttattgg acctaaaggt
    15781 ataattggta ttacacctct tgccatagtt gctaccatga ctaattcaaa tggaggtcta
    15841 tttgctgctc ttgcaggtga atatggagac tccacagatg ttggagcaat atctatctta
    15901 tctctaaatg atggtccttt ttttacaatg cttgcttttg gagtaactgg acttgcaaat
    15961 gtacctatag tagcactatt tgcagcactt ataccaatac tacttggatt tatacttggt
    16021 aaccttgatg aagatatacg agaatttctt gcacctggta caacattatt aattcctttc
    16081 ttcgctttcc ctttaggagc agcattaaac tttaatcaaa ttataactgc tggactacct
    16141 ggagttgtac ttggtttagg agttactata ataacaggta tgggtgggta ctttgtaatg
    16201 aaacttatac gtgcagaaca tccagcagta ggtgcagcta caggtacaac agccggaaat
    16261 gcagctggta ctcctgaagc tttagcagct atagacccta ctctagctac aatagcagct
    16321 gtttcaacag tacaggttgc tgctgctatt attgttacag caatatgttg tccaatgtta
    16381 gtttcctatc tagataaaag agaaaaaaag aaaaaatcta tttttaaaac aaataaaaat
    16441 attgaagtaa attaatactt aaggaggtaa aattaatgaa tgcaattatt catgcaaatg
    16501 aaaaagataa tctagttaca tgtgtcagac ctttagttaa aggtgaaaaa gtttcaattg
    16561 atggaaagtc ttacatagta aatgataata tacctgtctt tcataagatg gctacagaag
    16621 atgtaaaaaa aggtgatgtt gtatacaaat acggtgaaat cataggaatt gcaacagttg
    16681 acataaaaac tggtgactat gttcatgtac ataacattga aagtacacgt ggtcgtggag
    16741 ataaaaaata ggaggaagtt aaaatgaaat taatgggtta tttaagagaa aatggtcagt
    16801 ttggtattcg taatcatgtt ttaattattc caacatctgt atgttcaagt gagacagcta
    16861 caagaatagc atcacttgtg cctggagcaa tagcaatccc tcatcaacat ggatgttgtc
    16921 aaattggttc tgatatagag cttacagcaa agacattaat aggatttgga aaaaatccta
    16981 atgttgccgc agttttagta gttggtcttg gatgtgatgg tattcaagca aaagaattag
    17041 catctgaaat agctacaaca ggaaaaaagg tagattacgt tgttatacaa gaatgtggcg
    17101 gaacattaaa aacagtttct aagggagctg aaatagtaag taaaatggct cgtgaagtat
    17161 caaaagaagt aagagttgaa tttggtatga gtgaaattac attagcactt gaatgtggtg
    17221 gttctgaccc aacaagtgga atagcttcaa acccttcaat aggcgttgca tcaaaccttc
    17281 ttgtagatga aggtggaagt agcatactaa gtgaaactac agaagtaatt ggtgcagaac
    17341 atttgttggc tacccgtttt gaggatgaag aaatgaaaga taagtttctt aaatttgtaa
    17401 gtgatgtaga aaaaagagca attgctatgg gagaagattt acgtagtggt caacctactc
    17461 caggcaataa agctggtgga ctttcaacaa ttgaagaaaa gtcacttggg tgtatgtata
    17521 aggcaggcaa taagccattc aaaggtgctc ttgaatatgc tgatattgta cctcctgata
    17581 aaaaaggatt atattttatg gatactccag gtcaagatat agattctatc actggtatgg
    17641 tagcaggtag tgcacaaatt gtaatctttt caactggtag aggtactcca acaggaagcc
    17701 ctatttcacc agttattaaa atcactggga atagtgatac atacaacaaa atgccagaca
    17761 atatcgatat aaatgcaggt agaattataa cagatggtgc taaaatagca gatataggtc
    17821 aagaaatatt taatgaaata atagaagtct gtaatggtaa acataccaaa actgaaagtc
    17881 ttggtcatag agaatttggc atttacagaa tttcatctac attttagatt gtaacaaaat
    17941 atcttttata aatataatac ttatgtagat acataagcta aaagttcttt ctaatactat
    18001 aaaaattttc tatactgaaa aggaactgta tcaaaattat tttgaaaaca gttccttttt
    18061 taacctcttt tactcaaaat attataaaat aagatttaaa caataaacgt atcttgatta
    18121 atctctaaga agacataaat aaagctaaat gtaaagtggg ataaataaaa tttaatatct
    18181 ttgtatgaaa agatatttgg tataatatgt ctgagagaat tcttatttgt aaacaaataa
    18241 aggatggagg atgaaacaaa agaatgaact ttgaaaacac aaaaaataaa aaaataatag
    18301 ctactttact attgacactc cttttaatcg tgtcattaac atgtgtcact tttattatga
    18361 atctaaataa agtaacacat aatgaaacag aacgatatct tgtagaagtt tcacaaagta
    18421 tttccactac aattaatgtg aaatgtaaaa gtatatttag gacactatat tctatgggag
    18481 atacttattt tgagattaat gatagtgaaa aagaagtcaa tagatactta tcaaaaaaat
    18541 taaaattatt tgaatttgat tggttaggta ttatagattc taatggaaat tttgtaagtg
    18601 cccttaaaga tacaggtaat gttaaaggac aagactttta tagaaaagcc cttcaaggca
    18661 aagaaacaat tactcctgtt aaatcacaat ctggtacata tggaatttta tattctgtgc
    18721 cactatacaa tggcgataaa attgttggag ctgttgttgc ttggaatact attgatacta
    18781 tgagaaattt attaagtgta gaaagctttc atggtgaagg attttcccaa attatcgacc
    18841 aagatggaaa ttttattgta agttcagcaa acaaaaatgc tccaaaaaat gtgatgaatt
    18901 tttttgacat gattaaacac caaggaaaac aggttgataa aaataaactt gaagaaatga
    18961 aaataaactt aaaaaagaac aaaactggtt ctatgtatta tacattaaat gatggtattt
    19021 ctaaggcaat gcactatctt ccattacaag aagaaaactt atacctcctt tcaattgttc
    19081 caacaaaaac agcaagtgca cagtttgatt tacttctaaa aaaagcaatc ttcatcaata
    19141 ttttaattgt tatactattt ttattgctta ttatattgat tatgtttata aatcaaaaga
    19201 acagggcaca acttataaaa atcgcctatg tagactctgt aactaaaggt tacagtaaag
    19261 cacgttttga tattgaagct aaaaaaatta tacagtcatc ttcatcagaa agctattcat
    19321 tggtatctat aaatattcag aaattcaaac ttattaatga ttcttttggt agtgaagcag
    19381 gagataaaac attaaagtac atacatgatg tcattagtaa ttatctagaa ccaggagaat
    19441 tgctttgtcg agtttctaat gaccagttta atgtacttat taaaactatt cctcaaaaag
    19501 aaattcttaa gtacatagaa aatatcacca atgatattaa tagtttcaat aaaaatgaag
    19561 aatgtaaata ttttatagct ctatctatag gtgtatataa aattgatgac ccaaatctac
    19621 ctttagtttc aattcaagac cgtgcaaatg ttgcacgaaa aaataataaa ggaaacattg
    19681 gaaatcatcc actttataca tgtgtttttt atagtgattt agagcgttta aaaatgatta
    19741 aagaaaaaga aatggaaaat agaatggagg aagcattaaa taataacgaa tttatagtat
    19801 atcttcagcc taaaataagc ctcaaagata atactattgt aggtgcagaa gcattagtaa
    19861 gatggcaaga cccaaataaa ggccttatac caccagacga gtttattcct ttctttgaaa
    19921 aaaatggttt tattacaaaa ctcgaccact atgtttttga aaaagtatgt agtatgataa
    19981 gaaaatggat agataatgga gaaactccaa tacctgtttc agttaatttt tcacgtgcta
    20041 atttaaataa tatagatttt ttagaaaaat acagaatgct tcgtaataaa tacaacattc
    20101 ctgctaagct cttagaaatt gaattaactg aaacacttgt atttgaaaat ctccaaaaat
    20161 taattaatat aatagaccaa atccatgaag aaggttttca atgttcacta gatgactttg
    20221 gaagtggata ctcttcactc aatatgttaa aagaaataaa agttgacaca ttaaaacttg
    20281 accgtgcttt ttttagctca cctaatgcag ataatattgc tgaaaatcat gtaatagaat
    20341 ctatagttaa tttggcaaaa aaattaaata tgaatagtat atcagaaggt gttgaaacaa
    20401 tcttgcaaat ggattatctt aaaaaaatta attgtgatat gatacaagga tatgtttttt
    20461 caaaacctct acctcaagaa atatttgaga aaatgacttt tggaaaaaaa ctagaagaaa
    20521 ccagtatgga tagttccgtt taaataaaat aaaaaacttg tctctataaa ataattttaa
    20581 tcattttata aagacaagtc tttttatttt ccaatttaat tttattttct aaatttctta
    20641 cactcggctt gttttaaatt ccctttttaa gaataataat acgattatca tagcaataaa
    20701 atctgcaact ggctgtgcca accaaacacc atctaaatta aattgaattg gaagtacaat
    20761 gattaatggt ataaataaaa taaattgtct taaaagagtt aaaaacattg aatgtttaac
    20821 tttacttata cactgaaaat acacaggtcc tacaatagaa atccccatta taggcaatgt
    20881 aattagattt aggcttatac ctcttactgc tatatccatc aaatctgaat ctttattaaa
    20941 tatacctaca aatacttctg ggaaaacttg tactactaaa aatccaaatg acaatatgac
    21001 aactgacact atgatagata atattaaagt tttctttgca cgctcatatt gttttgcacc
    21061 ataattgtat gatattatag tttgcatacc ttgattcaat ccaaacacag gcatcataaa
    21121 catcaataaa atagatgtaa gtgctgtcat agctccaata gctaaatctc caccataaac
    21181 ctttagagaa ttatttgtaa acatgtgagt tatacttata gctacttcca tagcaaaagg
    21241 tgtcattcct attgcaaata ccttctttac tatatcttta ttcaatttta agttacattt
    21301 ttttagtttt aaattggact tccctcttat aaaataatgt attacccaaa aaaatactac
    21361 tatttgacag agtactgttg ctatagctgc tccttttata cctaaattaa atacaaatat
    21421 aaatataggg tccaatatca ggtttaatac acatcctaca atcattgtcc ttgctgctaa
    21481 ttttggatta ccttctgccc taattgcatt attaagagaa aatgcaacta aattaaatat
    21541 acttccaacc aatatgacac tcatatagtc ttttgcataa aatattgaat ctttacttgc
    21601 acccaaaata aaaagtatct cttctaagaa aactaatcct aatatagtta taattaaaga
    21661 gataattata gataatgtca aagcattacc taatatcttc tctgcttctt ctctatttct
    21721 ttcccctaac tttatagaca atcttgtgct agcacctact gataccaaca ttccaaaagc
    21781 tattattaga gtaaatactg gcatagttac accaagacct gcaatagcta tagagccaat
    21841 accttccata gaaccaataa aagctctatc tactacatta taaagtgaag ttaccatcat
    21901 tgctattatt gcaggtatcg aatacctaaa taataaaatc catatttttt catgtcttaa
    21961 tgcttcttga ttctccatgt tgtcaccatt tacaccatgt cataagttat acaaattggt
    22021 ttatttgttc ttgggtctgt tgctatttct gcatcaatat taaataattc ttttagatta
    22081 tccttcgtca taacatcata tgcatcacct tcacaaacta ttttaccttt cttaatacct
    22141 atcatgtggt cagcaaatct tgctgcatta ttaagttcat gtattaccat gacaatagtt
    22201 cttccttgtt ttttatttag ttcatctaat aattttagta tctccaattg atgtgccaaa
    22261 tctaaatatg ttgtaggttc atccagtaat aatatatcag tttcttgtgc caaagccata
    22321 gctatccatg ccctttgtct ttgtcctcct gataaatctt ccatatttct atccttaaat
    22381 tctgcaattc cagtaacttc taaactccaa tttacaatct ctctgtcttc tttactagaa
    22441 ttaccaaatc cacttttatg aggaaatctt ccataagaaa ttaattctga aacggtaaga
    22501 ccactaggag cttgagggct ttgtggaagt acagccatat cttttgctat ttctttagac
    22561 ttctttttgt agatgtcctc tccatttata tatattttcc cactattagg gctaataatt
    22621 ctacctatag ttttaagtat tgtcgatttt ccacaaccat tagcccctat gatggtagtt
    22681 atttttcctt ttggtatatt taaatttaaa ttttttataa tatctgtgtt tccatatgat
    22741 acatttagat tcttagtatt tatacagttc ataacaacac cctttcctaa tcatttacca
    22801 acattaaata aataaagtac ggcactccta ttatagaaac tactatacct actggtattt
    22861 ctataggtgc tagaagattt cttgatatag tatctgctat taaaagtaat aatgttccta
    22921 tcaatgctga tactggtatt aatcttttat gctttggtcc tattattttt ctacctatat
    22981 gtggagctat taaacctaaa aatgaaatac tacctgctac tgctgtcgat acaccagata
    23041 aaattactgc atatattata agtcttcttc tttgtttttc tacatctaca ccaagactta
    23101 tagatacttc atcacccaaa tttaaaacat ctatatattt agatttataa atagttatac
    23161 ccataaaaat cactataaat ggaagcacag ctattacata tttccaactt gttccccaaa
    23221 tgcttcctga tgtccatgcc ataaccctat taaattcttg tgttgtaaat ttcaattgga
    23281 atatcgtcaa aattgaagta aaagctatgt ttacacctat accaataagt agtaatcttg
    23341 acgaatttat accacctttc catgcaagag aatatataag aaatgctcca aataaagctc
    23401 ctataagtgc aacaataggc attgtaaata cagtcaagtt actcatacct tcatatacat
    23461 taccattcat aaggtatata taaactacta caaacaatgc agcacctgaa tttattccca
    23521 aaattccaga atcagctaaa tcattctttg taactccttg aagtattaca cctgctgttg
    23581 ataatgcaga accaaccaaa agtgctatta ctattcttgg taatcttaat ttaaatattg
    23641 caatttcatg attacgctga ccctgcccta aaaaagtctt tataacatcc attggttcta
    23701 tagaaaatga gcccatattt aaactcaata aaaaagtgat aaaaataagc accattaaaa
    23761 ttgatataac aagtaatgtt ttagaattta tttttataat atttttcata gtacctattt
    23821 ctcccccttt ctaacaaggt atataaatat tggcactcct ataagtgctg ttatagaacc
    23881 tactggtgtc tcatatggag gatttatcat cctacttaat atatctgtat atacaagcaa
    23941 tacagctcct aatactaaag agctcggtat tatatactta taatctgaac ctactattcc
    24001 tttagctatt tgaggaacta taagacctat aaaaataata ttaccagaca ctgacacaga
    24061 tgctccagtc attaatatta caagtactat acatataaat ctaacaaact tagttttttg
    24121 tcctaatcct attgccactt cttctccaag acttaatata gttattcttg gagccataat
    24181 aatagctaaa ataaatccaa taccaccagc tacaaatagc aattttatac cttcccactt
    24241 cactcctaca agccctcctg atatccagaa acttaattgt tgagataggt taaaatacat
    24301 agatattgct gatgccaaag aaattagcaa tgtaccaaga gcgactcctg caagagctaa
    24361 ttttacatta ttaactcttc ctcttgattt agaacttata aaatatataa ataatccact
    24421 tatacttgct ccaataaatg caaacatcat tacactaaaa cttccatgta taagttgagg
    24481 aagttttttt tgaagtacca gtgatatcga aattgcgaat gtagctcctt gtgtaattcc
    24541 tataacagat ggttcagcta taggatttct cgttattcct tgcattattg ccccagaaac
    24601 tgctaaaaac cctccaacaa gtgcagctgc tattgccctt ggaagtctaa catcctttac
    24661 tattctcata tttattccat cttccatttt tatcaaacta tctataacag ttgatagatt
    24721 catatctttt gccccaatag ttatggataa tacaattcca aatacaagta caactatact
    24781 tactaatatc aacaaataag ttatattttt tttactcagc ttagtcaatt atcctacctc
    24841 cttttccctt ttaatcgtca gtataatatc atctattaag tattttaatt ttaagattta
    24901 ttaaaaatag gtgtgaaaaa tgaatatcat tatctcacac ctactaagtt aagaattact
    24961 ttgttaattc atttttaact gattctaata ataactcttt tccaattgga ttgtatgatt
    25021 gactgaaaaa tggagcagca tcaagtattg ttacattacc atcttttact gctcttattt
    25081 gagcccagat agcactattt tctaaatctt ttttatctgc ttctgtagca attacaacta
    25141 tatggtctgc atctatatct gttaaacctt ccatacttac cattggtagt gttattccat
    25201 cctgttttgg catattctta ggtctagcta atttcatatc atcattgatt agagtaccta
    25261 ttccaccatc actaaatacc ataaattgac ctgaacttgc caacactggt aaataagttt
    25321 tttctccatt cttttctata acttccttac ctaactttgt agctttttca tcatacttct
    25381 gtagccaact tttatcttct tcttctttgt caaataactt tgaaacatca gttaacttgc
    25441 ttctccagtc atttgcataa tctttcatca taactacagg agctatttct tttaattggt
    25501 catatatttt ttcttgtctt tgactcatta ttattaaatc aggatttact tctaatatag
    25561 cttccatatc catagtatcc atcattgagt gcccaactat ctttacacct ttaagctctt
    25621 ctcttatgta acttggtaat ttatcagttt catatgagtc tacatttgct gttgcaacag
    25681 gcttataacc tgctaataat agttcttcac tacttcctga tatatcaact atttttttag
    25741 gatttgatgg tatcttaacc tctcctttaa cagattgtac aactcttgtc tctgatttat
    25801 ctttatcctc tgtattctta tctgaacatg ctgtaacaag tactaacgta gttataatag
    25861 atataaatat tgcaagtgat tttatttttt tcatgattac ttctcctact tgtttattat
    25921 acttacatga tattgattat cataagcaca tatttattat agcatttttc tatttttagt
    25981 caatattatt ttgtaaaagg ataaaaattc ctaattaatc ttaattatat atagcttaga
    26041 taaggttttc ttatattaaa cagtattttt tttaagaatt ggacacaaat acaatgactt
    26101 ttcagttaaa caatgataaa atgcaattta tagataagga atttagtagg gaggattcaa
    26161 aacatggtac atttagtata ttgtgataat gcaggaaaaa aaggagaaaa ggtcttagat
    26221 aaaatcttgg atggaacaaa aacaatggtt attagaggtg ctgctggtag aaaaatacca
    26281 catagtagag tatttgaaga tgagttactt tattttatgg aaaaaggaac tgcaaaaata
    26341 tctgcaaaag ccactgttaa aaaggtagaa aactatgtta aattaacaga agatgaaatt
    26401 tctaaaattt taaatgaaaa tcaaccaaaa ttaaatttat ctgaaaagca aaaagttcgt
    26461 tggcataaaa aatgtttatg cttagttgaa tttgaaaatg ttgaagaaat aacacctctt
    26521 gattttgacc atcaaggaaa tatggatgat tggcttattg ttgaaaaaat tgaagatgta
    26581 gttgttggta ctagtatacc ttacaattat gagaagtcta aattctaaag tatcaataac
    26641 ataatactat attgctaaac tctacttaaa taaaaaaaga gtgcatttaa taattataaa
    26701 aattattttt atacactctt ttttaataat aattttaaat tacttacttt ctaatctttc
    26761 atatacatct ataaattctt ctgctaattg ttgaaaattc atagctgtca ttaaatggtc
    26821 ttgtgcatga ataagtataa cacttatttc agttttatta ccattagctt cattttgtat
    26881 caattctgtt tggaatttat gagctttatg aattgattct ttcgactttt caatacattc
    26941 tctagcttta tctatattcc cgctctttgc ttcttttatt gcttcaaatg ctagcccttt
    27001 cgcatcacct gcaaatccaa ttattccaaa agatatctct atcatcttct catccattta
    27061 ttcgacctcc taaatatctc tatagcttga tatgactaat ccattctttt ctaaaaattc
    27121 tttgactttt ttagatgtaa gtatcttgtg ctctttcgtt ctatcaatag cataagatgt
    27181 agaatttaaa atataatcat ctaaaaaagc tgggtgagac ataatatcca ctacatcata
    27241 cttcattatt tcgtctatat tttttataaa atattcttca cttacatttt ccttataaaa
    27301 actatctatt aaaggaacta ctttagaata ctctaaatta tattcaaatc cacctcttat
    27361 tggaagatta tacttggtta caatcttctc tattactggc tttaaactta ctaaagtatg
    27421 gatatgatgg tgactatcta aatgagatat atctattcca agccctttta ccctatctat
    27481 ttgagcacaa agttctctat aaatctcatc acaatccatt ttttcaataa tttcgtctgt
    27541 aatcctccta ataaaaaaac cgtctttatc aacaatagtt tttaagttag ataataatgg
    27601 tctaccacaa cttaaagtca tatgaactcc acaatttaaa gttttatttt ctttaagtaa
    27661 gcctactcca tgttctaccc caggcatatt tgccatcata gaagtagacc taactatacc
    27721 attattatga gctgaaatta ttccatagtt cacagcttca caatatccaa aatcatctgc
    27781 atttattata attttagtca taccataacc tactttctat agacaactat ctattaaatc
    27841 taggaagata atctttatga gcttccaaca tttcgtctaa tatagttttt gctaagtcat
    27901 cagatggaat caatgggttt atacaaagtg ccaaaagagc agattcataa tcgccatata
    27961 cagctgcttt agctgccaat acttcaaatg atttgatttg acttactaat ccatgcacag
    28021 aatctggtaa atatcctata cttattggtt ttggaccatt ttttgttatt acactactta
    28081 cttctactgc ttgttcatct ttaaaatctc ttatagaacc cttatttaat gtattaacta
    28141 cttggatatc ttttttgtca ttgtatatag aacttattaa attacaagca gcatcactat
    28201 agtaagcccc tcctcttttt tcaagttgag gtggttttac atctaatttt tcatccttat
    28261 ataattcaaa taattgttct tccaattctt taactacttg acctctagct tttccctctt
    28321 taaactctct caactcatcc tcaagcattt ctttagtttt atagtagtat ctatggtatg
    28381 gacaaggtat tgcacccaaa gattttacaa attcagcatt aaaatcaatt gcctttatat
    28441 tttgcatact tatatctgct ttgacaaatt tatcaattgc ttcttttgta acatcttctc
    28501 catctaaaga tacatttaga ccataaacca tatgattaag acctgcaaaa tccatactta
    28561 ttctgtcact ttctacatta aatagtttgg caacatcatt ttttaaatga attggcacgt
    28621 tgcatagacc tatatatctt ctaaaattag tatatttgaa tacagcctct gttattattc
    28681 ctgttggatt tgtaaaattc accatccatg catttggaca taattcttcc acatctttta
    28741 ttatatcaaa tataactggt attgtgcgaa gggctttaaa taaacctcca gcaccattag
    28801 tttcctgccc cataactcca tgagataaag gaatagtttc atcttttatt ctcgcatcta
    28861 aaagacctac tctaagttgt gttgtcacaa agtctgcatc tcttagagct tctcttctat
    28921 ctaaagtaag atttattttc atatctactc cagctttttt taccatcctc ttagctagat
    28981 ttccaactat ctctaatttt tcttttccag cttctatatc tacaagccat aattccttta
    29041 caggtaactc ttcatatcta tttataaaac cctctacaag ctcaggagta taacttgaac
    29101 ctcctccaat tgtaactatt tttagtccct ttttcatctc attataccat ccttacctat
    29161 aatttaatat actctacaaa accccataca tttatttaaa actctataca tttatttacc
    29221 gaaataaaac tacctagttg ttaattttca atttattagc ttccattttt tcagacgcta
    29281 tgacaaatgg aatatatatt aatattgata atattaaatt aaatagtgct aaagctccgc
    29341 cagtccagtc acctgtagcc attgctccac ctaatatagg aggtgttacc caaggtattt
    29401 taggtaaaat tactggtgct actaatccaa aatctattgc taagtatgat acagcactac
    29461 atattactgg tgcaagtaca aatggaatca taaatatagg atttaaaact attggaagtc
    29521 caaatatcaa tggttctcct atgttaaata ttgcaggtgg accacctaat actatcatct
    29581 ctttacgtct tcttccagct attaataaag ctactattaa tcccaatacc attccagaac
    29641 cacctaaata tacaaatgca tcaaagaatg cacctgcaag tacatggtat ccttccgttg
    29701 ctcctgcttg agccaaggca gcattttctc cacctaattt cataagtata gtttctgtaa
    29761 aaggaagtgc tatatttgca ccatgaagtc caacagtcca aagtatatga attataaatg
    29821 caataagcat tgtagaacct agactatcag ctacatttga tagtggtgct cctaaataag
    29881 tatttaaaat atctgttaag aaacttccat tagaaagcat ttttataaat attcctatta
    29941 cagtaaatat cattatagtt aacattcctg gtattaactt tgcaaatgat cttgaaactg
    30001 ctggtggaac tccatctggc atttttatag ttaatttctt taccttagat aatcttacaa
    30061 atatctcagt tgataataat cctattaata tagaagagaa taaagctgct gtacctaagt
    30121 aggtttgttg tatcattccc catccttcaa ctactgtagt tcctccctca ggaactattt
    30181 taccaatttg tggagccatt ataaaaaaga tagataaagc tataagccca gattgtaacc
    30241 catcactctc ataagatttt gctagaaaat aagcaacacc tattactaag aatactgcca
    30301 ttgtacctat tgctccccac caaaggtcgc ctccaaatgt ggtccagttt tctccaaata
    30361 tacttgccat caaatttttg tatgcttcca atggtaagtt atttatcaat gttcccaatg
    30421 cacctaccat agttataggt atcattacta taaatgcatc tcttactgct actaagtgcc
    30481 tttgagctcc tattttagca gctacaggca ctatatattt atccataaat gacatgaatt
    30541 tttccatttt acatcctcca gtacaacttt attttgaact atttaactct acagctctat
    30601 ctaacacagc ttttccatta catcttccat aatccatcat atttataact tctactggaa
    30661 tattatatgg ttttgccatt tcagttgctt ttcctaatac atatctaact tgaggcccta
    30721 aaagtaagac atcacaattt tctatttcat tttttaagtt cacttctgat atagcccata
    30781 tttttgcttc tataccgttt tcctttgctg catcctccat ttttgtaact aataaactag
    30841 ttgacattcc tgctgaacaa gctaacatta tttttatcat tttttcccct ccaaattaga
    30901 ttcattaaat attttttaag tcataagttt tttaaactat acatttaaaa tatgtttttc
    30961 tattaatttt ttcttaatgt atatatctta tttttaaatg tatatacatt taattcttga
    31021 tattattata taccaaatgt atatacattt caagaaaact ttttcatttt atttaaattt
    31081 tattcgacaa cagcattcaa agttaatgta tatccatagt gatatgtgtg tgagtactca
    31141 aaaatctcac tattttgtaa cttacaaatt tggcttatat ataaaagtgg ctctggttct
    31201 tctaatttta gattatccat acactcttca ttcgatatta cagcttgtac ttctctctct
    31261 gacatggcta tcttgtattc acacacttct tcaacaaaat tataaagaga attttcacaa
    31321 tgagtcttat ttaaatttgg aaataactta actggcatat aagtaatcat atatgaaact
    31381 cttgcatcat tggctaatct tactcttttt atttcccaaa cattttcttc taaacttata
    31441 ttcaacttac ttgataaatt ttcatctgcc tgaactattt caagagatac caatatatta
    31501 gatactttat agcctttact ttccatctct ttagtaaatc cacttacaga cgatatatta
    31561 tcatatatat tatttgcaat aactatacta cctacacctc tttgtctagt tatatagcct
    31621 tctttaacta gattattaag tgcagacctt acagtcattc tagttacatt aaattcttca
    31681 ctcagctgtt tttcagaagg gattaaatcc ccttctttca aactgtctga atttattaac
    31741 tctctaacat gattttctat aactttataa attggctctt tcatttaatt cactccttat
    31801 taaatctttt atataataag ataattttat atacaaacaa agaaaatgtc tatacattta
    31861 agataattta aattttattc agaaatcttg tacaactttc cgagttttaa taaagatata
    31921 aatagactta tcattaatat accttttaat atacttgata aggttatact ccaccataca
    31981 ccatttagac ccaacatttc tggtctagat ataaagtaag ccaatggtac tcttgcacca
    32041 gtcagtatag taattataat agaaggtata taggttctcc ctaatccttt gaaagctcct
    32101 gttgttatta tttctaaaca cataaaaaat tgagaatagc ctaatatttt aagataaact
    32161 gctccttttt ctattgcctc actttcatct ataaaaatag aaaatatact ctctcctgca
    32221 aacactaata ctaaagtagt tattatacca agtacagaag atacagttat agctattttg
    32281 caacctttat ttattctaga atacttcttg gcaccataat tttgtccaac aaaactgcct
    32341 aatgcaactg atagcccttc agccgtcatc caggaaattg cttctatttg tgagcctact
    32401 ttttgtactg caacagccac aggcccccat gatgctacta ttactcctaa tagcattgag
    32461 aatactgtaa acataccact ttgtattgct ataggtaatc ccaatttata cagaacttta
    32521 taatatttag tctctatttc tttaaatagt cttaatttaa aatattcttc tttactttta
    32581 actattatat atataaaaca tgtagtaact actatctgag caaatacagt tgcaatagca
    32641 gctcctgcaa caccaagttt tggagcaaat ccccatccaa aaattaatat agggtcaagt
    32701 ataatattag ttattaatcc tactgtatta atacgaaaag gagttttact atttccaaga
    32761 ccattgaaaa ttgcagtaaa tactggatta ataaaataaa ataccatacc tagagctact
    32821 attatcaaat actgtctaga catggttata acttctaagt ctcctaatct aaaaaaacct
    32881 atcaattgtt tattaagtac tattaaagaa attgtatata ttattgctag tataatattt
    32941 atttctattg ctgacttcat atatgattta gtagtgctta tattatttgc accaatactt
    33001 tgtgctactt ttatctctcc acctactttt gaaatcataa taaatgccat tgcaagccat
    33061 ggaaaaaaac cagctgttcc aactgcagct actgctttac ttccggcttt accaacccat
    33121 atcatatcta tcatgttata ccccatctgt ataaatgaag tccccattat tggaagtgat
    33181 agcttaagta atttttctgt aattcttcct tctgttaaat taagtctgtc tttcattttc
    33241 cacctctttt atattaaatt tatttgctta tatagctaga ttaatcattt tatttgattg
    33301 agttcactat ccttcaattt atattatcat atctcaggat tgcatctcaa tcatacttat
    33361 gtttaagata cagtatttta cctatctatg ataaaattaa tacttatgaa tataaatcta
    33421 gccatttcat caatacatat tctaaccctt aacttaactt caattaagta tacatatcgt
    33481 aaccaattag aatattttat acggtatctt ccattaataa acaaccaata tcaatgattt
    33541 atatataatt ttatttatac ataaaatagt atactataat aataaaaaaa ctatatttga
    33601 atgctttaag catgtttact aattgtaatt tccacacaaa tttgataata tatgaatata
    33661 ttatggtgaa atttatagat atatattaat tatggggaga ttgtgggagg ttcttttcac
    33721 aagacattta agctatgaaa ataaataaaa aattcaatag agttgatatg caggtttcaa
    33781 tacttactgc aattattgta ataatatctt cctcatgtgt atattttttt aattatacac
    33841 taacttataa gagcatgata tactctttaa gtgagaggtc aaaacatatc tatgaatatg
    33901 tagaaagaaa tattgataaa aataccttta ctgaaattaa ttctaaagaa gaccaatcaa
    33961 aaaagtctta taaaagttct aaaatccttt tagaatctgt aaaaaatact actggagtca
    34021 tgtatctata tactgctaaa aaaacaaaag atggatcact agtatatgtt gttgatggtt
    34081 taaattcatc taggagtgat tttagaaatg ctggtgattt gatagaaaaa gagatttggg
    34141 gagaccttaa caaagcatta gataatcata tattgttacc taaaaagatt aaatcaacat
    34201 cttggggata catatttata tcgtattttc ctattcacaa tgatgttggt aaggttgttg
    34261 gagtaatagg aattgaattt gaagcaaaac atcaatacaa tacatttaaa tttataagta
    34321 tagttacacc aataatagca ttattaactt gtctaatatc agcattgatt gcagtgattc
    34381 tatttaaaag aatatctaat cctacatata ctgattttgc taatacagat tatttaacag
    34441 gcctaaaaaa tagaaatgct tttgaaatag atatgaataa cttaaacaat tcaaatctaa
    34501 agaatctaat atctatcata tcaatagatt tagacggttt aaaaaagatt aatgataaat
    34561 ttggccatca gacaggagac ttatatatta agcaagcaag taaaattata caatatgtaa
    34621 taaataaaaa a
//
LOCUS       DAEMGG010000029        34514 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEMGG010000029 DAEMGG010000000
VERSION     DAEMGG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.029"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            188..1525
                     /locus_tag="KPK24_001386"
     CDS             188..1525
                     /locus_tag="KPK24_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2299071.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(1861..2430)
                     /locus_tag="KPK24_001387"
     CDS             complement(1861..2430)
                     /locus_tag="KPK24_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG2299072.1"
                     /translation="MKKRLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYFNRYN"
     gene            complement(2782..3795)
                     /locus_tag="KPK24_001388"
     CDS             complement(2782..3795)
                     /locus_tag="KPK24_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2299073.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQVNVY
                     IDN"
     gene            complement(4407..5309)
                     /locus_tag="KPK24_001389"
     CDS             complement(4407..5309)
                     /locus_tag="KPK24_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG2299074.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKDRGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            complement(5436..5555)
                     /locus_tag="KPK24_001390"
                     /pseudo
     CDS             complement(5436..5555)
                     /locus_tag="KPK24_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5718..5867
                     /locus_tag="KPK24_001391"
     CDS             5718..5867
                     /locus_tag="KPK24_001391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299075.1"
                     /translation="MQAQTISYPSALSVKVYPLPLPIACSVLPSRGIVVEVSPIRSLP
                     ADCPF"
     gene            complement(6101..6763)
                     /locus_tag="KPK24_001392"
                     /pseudo
     CDS             complement(6101..6763)
                     /locus_tag="KPK24_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(6923..7459)
                     /locus_tag="KPK24_001393"
     CDS             complement(6923..7459)
                     /locus_tag="KPK24_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG2299076.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEETKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(7532..7945)
                     /locus_tag="KPK24_001394"
     CDS             complement(7532..7945)
                     /locus_tag="KPK24_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2299077.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            8438..9076
                     /locus_tag="KPK24_001395"
     CDS             8438..9076
                     /locus_tag="KPK24_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG2299078.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEGTI
                     SDMQSNINGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISS
                     MDGTINSMQDSITDMQVEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIK
                     SDVKINRNTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(9684..9863)
                     /locus_tag="KPK24_001396"
     CDS             complement(9684..9863)
                     /locus_tag="KPK24_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2299079.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDKSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(10089..10622)
                     /locus_tag="KPK24_001397"
     CDS             complement(10089..10622)
                     /locus_tag="KPK24_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG2299080.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQITVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            10939..11289
                     /locus_tag="KPK24_001398"
                     /pseudo
     CDS             10939..11289
                     /locus_tag="KPK24_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11920..12492)
                     /locus_tag="KPK24_001399"
     CDS             complement(11920..12492)
                     /locus_tag="KPK24_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2299081.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(12554..12817)
                     /locus_tag="KPK24_001400"
     CDS             complement(12554..12817)
                     /locus_tag="KPK24_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG2299082.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(12817..13038)
                     /locus_tag="KPK24_001401"
     CDS             complement(12817..13038)
                     /locus_tag="KPK24_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG2299083.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            complement(13551..14087)
                     /locus_tag="KPK24_001402"
     CDS             complement(13551..14087)
                     /locus_tag="KPK24_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG2299084.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(14105..15418)
                     /locus_tag="KPK24_001403"
     CDS             complement(14105..15418)
                     /locus_tag="KPK24_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG2299085.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            15773..16213
                     /locus_tag="KPK24_001404"
     CDS             15773..16213
                     /locus_tag="KPK24_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG2299086.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQIPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(16398..16586)
                     /locus_tag="KPK24_001405"
     CDS             complement(16398..16586)
                     /locus_tag="KPK24_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299087.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            17590..17985
                     /locus_tag="KPK24_001406"
     CDS             17590..17985
                     /locus_tag="KPK24_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299088.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            18028..20127
                     /locus_tag="KPK24_001407"
     CDS             18028..20127
                     /locus_tag="KPK24_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG2299089.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEDSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(20352..25562)
                     /locus_tag="KPK24_001408"
     CDS             complement(20352..25562)
                     /locus_tag="KPK24_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG2299090.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKEIM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNALYEEHKDWIISKREDSGSA
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YTEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVNLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(25577..28072)
                     /locus_tag="KPK24_001409"
     CDS             complement(25577..28072)
                     /locus_tag="KPK24_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2299091.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(28755..29042)
                     /locus_tag="KPK24_001410"
     CDS             complement(28755..29042)
                     /locus_tag="KPK24_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299092.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(29062..29337)
                     /locus_tag="KPK24_001411"
     CDS             complement(29062..29337)
                     /locus_tag="KPK24_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299093.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(29974..30153)
                     /locus_tag="KPK24_001412"
     CDS             complement(29974..30153)
                     /locus_tag="KPK24_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299094.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGHAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(30438..31913)
                     /locus_tag="KPK24_001413"
     CDS             complement(30438..31913)
                     /locus_tag="KPK24_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2299095.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
     gene            32982..34160
                     /locus_tag="KPK24_001414"
     CDS             32982..34160
                     /locus_tag="KPK24_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2299096.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFRNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
BASE COUNT        11957 a         5820 c         3770 g        12967 t
ORIGIN      
        1 tttaattttt tgaaacttaa tttttaaaat ttcaatcttt taaaactcaa ttttttgaag
       61 cttaatttct agaattttat ttttcaaaat caattctttg aaatctaatt tccagaattt
      121 taatttttaa gatttaattt tttaaaactt agtttccaga atcttaatta atagaaaggt
      181 atattaaatg ggcacttatt ctttaatctc tacaagcata aattttattc taatattaat
      241 caatattttc atagcctttt ttctttataa gttgacttcc tattttatag actttaggaa
      301 tcattggatt ttcaaaatca tattgatttt gggctactct attattagtc aaatgataat
      361 atggctttcc gaccccgtaa atattttgtt tacactattc ggatatttta tactacttat
      421 aatatgtagc aaaagcaaat taattcctat aatatcagtt gtcatgatac tttatcctat
      481 tataattggg cttaattttc tttttataaa caaccctata tatgaggaac ttatacactc
      541 aaaagagtat attttaatac tttcaatagt ccagataatt tctgctctaa ttaaattatt
      601 attttggttt ttaatacata ttttcctaaa gaatagattg gtcaatataa aacaatactt
      661 atcaaataaa atatggattt ttattgatat ggtgtgtata tcttcatttt tatcaatatt
      721 aattgctatt attctgccac ctactaattt aatccctatg ggtagtaata agaatattat
      781 cttaggcaac ttaccttatg gcacttatct aatcatcttg tcagcaattc tggcaaatat
      841 cggtgtgata cttcttttac agccattgat tgaaaatgta aagatgaaga tagaaaaaca
      901 agcaaatcac ctaaaagaaa aatactataa cttactagaa aatcaacaaa ttcaaatacg
      961 caaaatgcga catgatatga ataatcactt tcaaatgatt gaatcgtatt tagaaattga
     1021 agatattgct ggagctaaaa agtattttaa tcagttaaaa ataggtatag gtactcttag
     1081 tggcaaacaa ttttgcaaaa accaagctct taactcaatt ttaaatcttc gtcatacaag
     1141 tttggaagaa aatggtgttg atgtgcactt taatatagat atagacaatc acttgggtat
     1201 agaacctata gatttatgta ctattttttc aaattcttta gataacgcta tagaagcatc
     1261 tttgaaaatt cctgatttgt caaatagaaa agtggtatta aaggcacgtt gtgagaagga
     1321 gtattttagc tatttattga caaacaacaa ggtaaatcct atcaatagaa aacaagaact
     1381 atttatttca gataaaaaag atagtaattc tcacggatat ggtattgaga atattagaga
     1441 aattgttaat aagtacaatg gaaaattgga tatttcttat actgagaatg aattttcttt
     1501 atttctttat attagattat tttagtctaa aattagatta tcttatataa tacaaattag
     1561 attgtttgat actatgtaaa attataataa cttataatta aatatttaca catatattca
     1621 ttaacttaaa atttaatcaa atgtgatatt ttatttacct tagtaaaaat gtgtgtgtct
     1681 caaaataaac tttttagttg atgaggcact cttttttaca tgaaatcaaa tatattttta
     1741 tcctttcaac aatgaaaaag ggatttatct ctccattttg aaacaagccc tttttctatt
     1801 aattactttc ttaaaaaata taatacaata tataagataa atatctaatt gtacctattt
     1861 ctaattatat ctattaaaat acaacacctt tgccttttca gtaaactccc taacaaaatc
     1921 tagtttacta tctgtataag catcacgatt atgctcatac ttcttccaaa ggtccaactt
     1981 aagtttttca tactcctttg caacatctgg gtggtcaatt aagtaatctc taaaaaacaa
     2041 ctcatcatta tcacctacat accttatatg caaatgaaaa actcgttctt caaacccatc
     2101 tactgtatac cctttattaa gtgaaagcct actactgctc tcagacatac aaatatatcc
     2161 attcgactca atcaaatctt taatatcttt catattataa tcaggaatta tctcaatcag
     2221 tatgtcaaca atgggcttgg cataaatcgt actcacagaa gtacttccaa tatgacttat
     2281 tctaacaatt ttttgcattg gtaatattcc agttagtcta tattgttctt ctttaaacca
     2341 atttttccaa cattctttgt gagtagtcaa aaatatagga aacaagttcc ataactcctc
     2401 taaagtcata tcttccaacc tctttttcat tagttacctc cttttaaatt tgtactttat
     2461 attttttctt tttcaaattt acttagttaa tatttaactt tattttttat caaatcattt
     2521 ttctaattat gtaaattttt ctaaatttag tttttaatgt attataacag atttttaccc
     2581 ttcttacact tttgcatttc tttaaatatt agaacatttt taatctacaa ttgtagtaat
     2641 tttttcttta cctgtattta ctggcattgg agcttctaaa gaataattca ttggctttaa
     2701 tataattaaa gtaaatttaa catatgtgaa agatacttat taaaattatt tatattaatc
     2761 tcttgttaaa ctttgtattt tttaattatc tatataaaca ttaacctgat atgtaggttt
     2821 tgaattagtt ctgttttcaa taggtgtttg ttttgaaagt ggataaaata ttgatttagt
     2881 agtaattaaa tcaattttaa aatatgtaat agaagataat aaacataaaa taactgctat
     2941 caaaatacca tttctgtact tttcattaaa catactaaac actctttttt taatatctga
     3001 ctccttatgt aatccagcca caaatttatt tgaactattt gaaccttttc tcattgcact
     3061 aattattgtc aatgcataat cctgcttctc ttctatcgta caattcttca aaactctttc
     3121 atcacaataa aattcacaat ccaaatctat aaacttctcc attatataca caaaaggatt
     3181 aaaccaataa acacatttta aaaatatcac caaatactta atataaaggt ctttactctt
     3241 aaaatgcatc aactcatgcc taaatatcca gtttaactct tttaatgtat aatcacatgg
     3301 tggtaaaatg acacacgaat taaaaaatac tggactttca acttcatatg aatatttaag
     3361 ccgtattttt ttatttatct taagctcttt taataaattt tcataaagat tttttatctt
     3421 atcatcctca acatcataag acaaatcatt tatcatattt ttaagcttaa tatatttcaa
     3481 taaagtataa actaaataaa atatcgtaat agcaatccaa acagagacta aaacagtact
     3541 atatacaata gaaatactat taaaattgcc taaacttatc ttattaatat taccattata
     3601 ttttgactca ttaaatataa aagtataatt cataaatggt aatgatagct taataatcac
     3661 aataagccat atatagtaat taaatttttt actgaaaatc ttaaataaag ttattctaaa
     3721 gactaataaa ataactataa aaatacaact tactaaaatt gttctaaaga tatcatttaa
     3781 aaatgaacta accacagata ctcacctttc ttatactttt atatttatat aatcatctac
     3841 acgaatacta aactgatatg taggttttta attaactttg ttttcaatag gcgattgtct
     3901 tgaaagtgtt ttaaaagttg atagaacaat gatttaggag taataaggtc aattttaaaa
     3961 tatgtaataa aatataataa gcatgaaaca actgctatca agataccatt tccaaactct
     4021 ataagcaaaa ggggctaata caaccttaac cccttttaat tattttcatt tataaataaa
     4081 ttctattctt cccaaaatat cgcaattaaa atatattaat taaaatatcc caataagaat
     4141 atctgaaaaa tcttctacct attttttcaa accctttatt cccattcctc atacaatagt
     4201 acaactttta gcacagtata aatataccat tagtccatat aatctcatta acaaatatat
     4261 acaattattt tcttcaaact cttttacatc taagatatca cattttcatt ttcttatatt
     4321 aattccttaa ctctctttca aattaccaat tttttaatca aatcttcatt aaatattcta
     4381 tcattattaa acattcatca ttctccctaa aaaatatgtg ccgtatgttc tttacacaaa
     4441 ttttccatct tatcatacac agcaccacta tctctctcat ctgtcatagt cacaattaca
     4501 tcacttggtc taagaattgt cttacctcta ggaataattt cttctccacc tctttgaatt
     4561 gctaccaaaa gacaattttc aggccactgt acatcctgaa ttgccttgtt cgcaacagga
     4621 gagccaaaca taacagagaa ctggtctagt atcttttcgc ccctatcttt atgtacaggt
     4681 tctccatttt tttcaagtaa cctctcaaga agactttcat aaataggttt agacttcata
     4741 agtgtagcca caatatatgc cacaattgaa attactgata gtgaaagcat ttgactaaca
     4801 gaacctgtca tttcaaaaat taatatgata ccagtcaaag gggcacgcac aatagctgta
     4861 aaatatcctg ccatagccaa aagcacaaag ttatttacat aatcgggatt cattccaaaa
     4921 actttaacac ctaccatacc aaatattcca cctataaatg ctcctaaaac aagcaatgga
     4981 aaaaatattc cccctggtgc acctgaccca aaactaacag ccgaaaatat aaaacgtata
     5041 acaaaaatca aaacaaccat tccaaaaata tattcatgac ttgtaagttc ttcaactaaa
     5101 ttgtgtccac tccccagaac atcaggcata acaagcccaa gaataccagc cattataaat
     5161 ggaatcaaca gttttccaaa ggtaccaatt ttaggaatct tcttgtataa agactgcaca
     5221 tatagagtaa accagttgta aaatgccccc ataactccaa gaataacacc taaaatcact
     5281 attaatccat aataatcttg tggcaacaca tttccaatct gaacctccca tgactaaagt
     5341 cacagggttc ctgcttcata gaattttgca tactaacata tgtcttacta attctccaca
     5401 ggctatcccc gtagttccta cggttcttac ataagttatt tattttgaat tgttattagt
     5461 cttaatcctt cttttaatat atttatactt gcatttatat ctctatcgtg agtttcatta
     5521 caactaggac aaatccattc tcttataata atgttaacaa atgtaattat ttaaaacaat
     5581 tatttagaag gttttatatt taattttaaa taacatattt aagaaccgta agtcttaact
     5641 tttatcattt ttgaggttgt cgttcacata agttcgctac tacctatgca gttctcttat
     5701 gaacttctta cactttcatg caagcacaga ctatatctta tccttcggca ttatccgtta
     5761 aggtctaccc acttccacta ccaatagctt gtagtgtact tccctcaaga ggaatagtcg
     5821 ttgaagtttc tcctattcgg agtttacctg ctgattgccc attttaaagt acttaggatt
     5881 taaccttata ccatctaacc aattttttct actttcgtaa ctttcacact taggtttatt
     5941 tcatccttat gttttagttt ggttagcttt aggggtttcc agcaattcaa gtagtattgg
     6001 ataggttcta tcctatctct acatataggt ttccctatat gctgactatt ttagctacta
     6061 actcatgact aaagtcacaa gtgtgcgtag ctatttttta atcaaactgg aatacagact
     6121 ccattcctag gaaattggct gaaatatagt ctgctgtcaa agatgctgtc atcacagaaa
     6181 ttaaaacaga tactgaaaag tttttatgga cttcttcaag tgaaaacata acacctgcta
     6241 gtggtgcatg aaaggctgct gataatccag cacttgcccc acaggttaac aggaattttt
     6301 cctctgtctt ccctcggtca agagctctag aaataccttt acctgccata gcaccaagct
     6361 gaatggaagg accttccctt cccaaagcaa gaccacccat aagtgataag aatcctccaa
     6421 taaattttgt tggcaacact cgccaccata cttgttttat tttccctgtc atttccccct
     6481 caagctgagg gataccactt ccagaaatca ttggctccca gtccaccaac tttgatacaa
     6541 tccacgcaag tactgcaagt actgcaaacc aagctattat ttttattgta gaaccttcta
     6601 caaattttaa aatttggcta agccaagtac cagcctgccc taaaagtatc ctgtataaaa
     6661 gcacaatcaa accagccaca gccccaacca taagtccttc accaatcaaa attacctgaa
     6721 agtgctctgc ccgtttgata atatgagatg tatccttttt catactgttc ctccttcatc
     6781 caataattct acacaatctg aatctccaat gactaaagtc atgagtgtgt acagatattt
     6841 tttaatcaac aaaaatagat ttttatttac taaatataag aaaataattg ctcaatttct
     6901 caataaatgt aaatttattc ttttaggtat ttattctcca cagtcgtgct tcttccagca
     6961 aaatatttat tagttcttgc tttctctgtg tatagatttt acgattgtcc tcaaactgta
     7021 atgccaattt ttgtttatga ctatcataga gttttgcttt gtctgtaaaa gtatttagat
     7081 aatctcgaaa attgatataa ttattccatg caacactatt ccattcaaca acatgaatat
     7141 ggtgagtacg agtatttttc tcaaaatctc ccatagcaaa aagtaattgt tttggtacat
     7201 cctgacctcg aaaaacgatt ccacattgct ctaacctttc gaggtataat ttagtttcct
     7261 ctaaagaagc aacacctaca acaatatcaa taataggctt tgcgctaata ctgggaatag
     7321 aagtgcttcc aatgtgttga atatcaatgg cagtcttatc ccatatactt ttcaataata
     7381 taatgacatc ttttgcactt ttatcccata tcatctgatg ttccaataat ttaaccatac
     7441 cacattttaa acctatcact atataccacc tcaatataat ccacctttat atatattaca
     7501 attttttcta tcttgataaa cagtgattta gctatttcac tttttcttga cactcgctac
     7561 attccctatc atgaatagat ataattcctc cacatttaga acaagtatat ctctccttct
     7621 gcttttgcat aaacttctct aaaccgtact gctgaacaaa tttactattt tccataaggc
     7681 ttgcttgata tctcttgtta taactttttt caagattttt tatgagttta caaggaaaat
     7741 tgaaacattc aaaacaataa aacaaccctt tttgacagac acaatccttt attttacact
     7801 tacggcagtg ttctggcttt cctgtatcgc cattcaaaca accagcacat ggctttttgt
     7861 gataacaatg cttatagcaa accatacaat tcattccaca aggagcaaac ataatcgtat
     7921 caatactctc ttttggcatt ttcataacat tttactcctt tcaaaaatcc aatttgtcga
     7981 attgttatat attcatattg taacataaaa aatagctaaa aaattcatgc actttcattg
     8041 ccaataaatt ttcaaggaaa tatgaaagtt taaaactaag aataaaaaga gataaaaagg
     8101 aagctactaa tcaaaatatt caaatacaag taatcaatat aaataactta aggtatattt
     8161 aaaagtatat gatattacca gcagttttaa atacaaatat aaccacacat gcctcaaaaa
     8221 cacttattac agtatcttag aaacttagac aatacataag attcattaat aaaaaagttc
     8281 tcaatatagt cattaataaa aacgttctca atatagatat atttaaccat gttttgaata
     8341 gcagataatt atatgattaa aaattaaata tgacatagcg tttgtggaat ataacaccta
     8401 aattatgtta taataataaa acaggagtgt gatagtgatg gatgaaaaaa tattagagtt
     8461 attacaggaa atgcaaggta atattagtaa tatgcaaggt agtatcagtg gtatcgaagg
     8521 cactattagc gatatgcaaa gcaatatcaa tggtatcgaa ggcactatta gcgatatgca
     8581 aagcaatatc aatggtatcg aaggcactat tagtagtatg caaggcaata tcaatggtat
     8641 cgaaaacact attagtggta tgcaaggcac tattggcgat atgcaaggta gtatcagtgg
     8701 tatgaatggc actattagcg atatgcaagg cagtatcagt agtatggatg gcactattaa
     8761 tagtatgcaa gacagtatta ctgatatgca agttgaaatt aaagaagtca atagtaggtt
     8821 agacaatatg gaacctgata ttaaagactt aaaaactgga caagttgaaa ctagtagtag
     8881 acttgatact attcagagtg atgttacaga aattaagtca gatgtaaaaa ttaacagaaa
     8941 tacattagat acagtgatta aacatacctc ttcattagta aaagatacat cagatattaa
     9001 aaatgattta aataatatag agatagtcac atctaagaat tcgaatgata ttgctaaatt
     9061 aaaatcagtt aaataatata ctatagtaaa aaatacctga aaatttcaca tacttcttta
     9121 aagttatacc aaattttata cagaaatact gattatccga caacacactt tacttataaa
     9181 taatttatta aaaaaatact cattttatcc taaaattaac acgactaaat gagtcttgat
     9241 ataataataa aaaataaggg agattggaca ctaaaaagtt gtgtatctcc cttatattct
     9301 ataatatagt tatataattt ttcagcctaa aataaaagtc catctttgat taaaacacat
     9361 ccccttatca cttataaaag gataaaatct tgttttcatt tttccattta tatcttcttg
     9421 cttaagagta ataatttttt cttttgatac tttaagtgtt tggttaaatt taaccaatcg
     9481 actaagtttt gttcaacatc tatttttcat attttccaat acttaatcta ttttttataa
     9541 ataattcttt catttctaac tcacttacat tttttacata aaacgaagca tataaatttc
     9601 aaccttatct tgtatggttc ttaatactta atgagtagct taagtatcaa acattataaa
     9661 ttttcccctt acattcaaca ctcttaccat ttccaaaata tacaaaatat attttgaaca
     9721 tttgtacaat gatgaaatac aatacatgaa aatacaatat cataactctt gtctaaaaat
     9781 agaagttctt caacataacc tttcacaaaa aacatattat ctaagtattt tttgtctgct
     9841 ccttgttttc ctgtttgtag catataaatt actgcatcta aacatataac tactcacacg
     9901 aaaaaagaaa ataaacttcc aaaatacaag ctctaaaaac tacctctaaa gttgtataat
     9961 gtttactagg aataaggttg tataacatat agtttaaata atcatttttt aaaattgcaa
    10021 gttattgctt tcaaaattag atgtttgtat atcaaataaa tgattaagaa tttctaaatt
    10081 taatggcttt ataatgcact ccctttcttt gggataaaat aatgttgcac agatactttt
    10141 tctaaagata acaattttat ttttctatca attccaccag cataccctgt caaactccca
    10201 tttgttccta caacacgatg acatggaaca ataatagaga ttccattatg ccctactgca
    10261 ccaccgattg cttgtgctga cattttagat attccttttt gttgagcaac tttatatgca
    10321 atttctccat aagtgattgt tttaccatat ggtatttgtt gtaaaatttt ccacactgta
    10381 atctgaaaag gagtacctac catatggagt gatggtgtaa aattgggttc ttttcccgaa
    10441 aaataaatat ctaaccattt ctttgtctgc tcgaaaattg gcaattcttt ttcttgatgt
    10501 tcttggtcaa gattataagc ataatacttt tgattctcaa accaaagacc agttaaccca
    10561 atattatctg atgctaaaag aatatttcca attggactat ggtaatagct aatatattgc
    10621 atacaattcc tccttatatt ctgaaaactt cactcaaatc attagacacc tcttttctat
    10681 ctaccattat ataaaaacat actaatataa tttccttcta actacacttt cgttataata
    10741 tctatcctca tatcatatta gttttatatt tacctgcatc tgttcatcct tttttccctt
    10801 tccctttgat gaatataaaa ttcatacatt taagcttcct actgtattaa cttttataga
    10861 agtgtatatt ataattttat caccaaatag accaaaaaaa tatcgatata acatcaagta
    10921 atatcataat actttcaaat gggaaaagta agaataggct gtaaaaaaga aaagtatttt
    10981 tatgttagaa atccagtaaa atcgttagta gatagaataa cagaaaacga acaatttgct
    11041 atttatcatt ttacacataa atataattga cgcaataaag tgttttctat tctacaacga
    11101 ttaataaggt tagaaaaaag taaaaatgaa ctgtatatgt atgaagcatt agtttctgtt
    11161 tctttccttt ggcttattat gataaaaaat attgttttgc caccaataaa acacaaaaat
    11221 ataattaata tccatatgca aaaaatttta cgttatattg aaaaatacta ttgttttaca
    11281 ttatattgaa gggtactatt gtgaaaactt aattttggag tactatcaag cagtggtaat
    11341 atcaataaat cagagtgctc cagatgattt gaactaagtt taaatactat accttataaa
    11401 tattttactg agttttgttt attaaaagca actcaatttc taaaaggcac aaatgagcta
    11461 ataagtgaca ttactataaa agttgttttt catcaaataa tctattttag taaatgtttt
    11521 aaagaaaaaa cgggatattc tccaaaagtg tacagaaata taaaaaaaga gatatgattt
    11581 tttatttctt ttgattatcc aaagagtaaa taaaaaatat tgtaaaaaac ttactaacaa
    11641 cggcaatgct ttgaactatg gtaatacttt gaacttttga ttggactttt tttgctaaaa
    11701 taatatattt aaatagcaaa gtaatctgtt taatcaataa aaaataagag ataaaagaaa
    11761 tcaactaaaa cttttacctc ttattttgat agtttatcag agctaatatt tacactcttt
    11821 ttatttttga tacttcatta aaaatgatgc ttactctcca ttcactttta attcttcatt
    11881 aaaaatgatg cttattctcc attcactttt aattcttcat taaaactgat gcttgcactc
    11941 tgtaaactca taaacatcca ttaatgcttc accaaatctt tgatagtgaa ctacttccct
    12001 ctcacccaaa aatctcaata tatctttcaa atcagggtca tcagttaaat ttattaattg
    12061 atagtacgta gccaatgctt tttgttcagc agccatatcc tcatgtaaat cagttaccga
    12121 atcgccaaag acattaatat aacttgtagt aaagtttact ccattagaat ctactggctg
    12181 aagaccatgt ccaaaattag cataattact accaagtcca gcagccttaa gctcttctgg
    12241 agtacaattg cctattaatt gatataacac tgaagaaatt atctcaacat gagccatttc
    12301 ctctgtacct atatcagtta aaagtgcacg agattttcca gttggcatag tatatctttg
    12361 ttgaagatat ctagttgcag ctgccaactc accattaggt cctcccaact gagtcatgat
    12421 atattttgcc attctagggt ctgcttgtct tatgttaact ggatgttcca gtgttttttg
    12481 ataaatccac atattatctt actcctcctt aatatatatt taataataaa tctaatttta
    12541 aagtttaata attttattta taaaatgatt tgtcccatgg ccaaggttca gatatccatg
    12601 accaaggata ttcacttgat tcatatccaa aattagttaa tgctccatat ttagattcat
    12661 aatcacacac agcttgtgta aatttatcgc agtatctgtt gtatgaatca agagctttct
    12721 tatcccttgg attgttatct aaatataaat ttaattctat acaggcaaat tgatattctg
    12781 aaattctctc taaaagttct cttctattta atgacattag tacatgttac cccctttttt
    12841 agctctaact ctatttttag gatttgcata tagccaatca ttatagttat ttgactctat
    12901 taaatctaat tcaggaaata aagtacctct ttttaatgct gtttttggat tgtatgtgtc
    12961 atcataaatt tgacgattga tgtatggtct agcaaattca caacaatagt cttgtttatc
    13021 ctcttttaaa tatcccatta attatcctcc ttaattttta gaattttaaa atagcattat
    13081 ttttaaaaat tgtatactgg ttctatatta taactggtag ttttaatagg tctttaataa
    13141 gattaaatga ttaaataagt atcttttttt cttattttac ctctaaaatt tttctaatta
    13201 atctttcata aatatctatt ttaaataaat aatatagagt ttatacaatt ttttatagct
    13261 taattactat ttactatata ttattcattt ttaaataaaa tgtgattatt ttttattaaa
    13321 aacaatataa atatacaatt taatactatt ttttaaataa gcttatatta aaatacattt
    13381 agttatataa ttttatttat tctaagagta taattataaa aataaaaaaa gaataggata
    13441 attttcctac tcttttttct gtacttacat ataatattaa aactctttat tttttaatat
    13501 atattttaaa ttttcttttt taataaatct ttttctttta ataaatctat ttaattttct
    13561 tcttctatat cagagccaaa gggaatccat gcattaccaa actctttatc tttaaataaa
    13621 taagctaatc cagttatttg cttcaatcta agaatttcat caacttccat acccaggtgc
    13681 tttgatatcc attcatcaga ttttccaatt tcacaaagtt cagttataat tttactcatt
    13741 aaatccacat tatgagaacc tcttgcacga ttgtgtcgaa tagtacttgc cattctttca
    13801 tcaagagatt tatcaattac tgaaacagga agaagtccat tttctctatc atatatatcc
    13861 tcattttcaa gcattactct atatctatga aaaccatcta caataatata cttatccttt
    13921 tcctcattat aataacagac aattggcatt gtatacccat cttcttttat acttttatac
    13981 aacaactgca tttctggtgg ggctactgca tttggattat agtcgttaga ttctatcttt
    14041 tcgattggca ctgcgatgat tccatataca ggacttttat attccatagc atcttaactc
    14101 ctatctaatg aattttttca tatttcttct taatatattc cgttctaatt ctctgttctt
    14161 ttgtaggacc aaatcccata aatctacaca tatggtcatt ttttaaaata caataacaca
    14221 ttcttttcca acttggtata tctttggtaa atttaatatc atccgtatca tcaggtatat
    14281 catccaaaaa tatgactcta ctctttttat cttttgtatg atttgaaact ccatttaatt
    14341 ctattttata accttttctc aacaattcac gtataactat ttctgacaaa ccaccacctg
    14401 tattctgcca aaactcaata gatgttgaaa attttttaat atagctattc cttatatttt
    14461 caggtaaggt atcaagcaaa aaatatgtga aacttttcca tgtgtgtcca tatggcaatt
    14521 taacactacg atatcccata gcttttgttt tgccataaat agaagtaaaa ttaactcctt
    14581 gtactctagc aaccaatttc acccatacat ctgggtccaa tattctgtat aaatttaaac
    14641 tctcttttgc tgaatcatta aaaggtgatg ccactctcat ttttcttgga gctactccag
    14701 ctttatagaa taagtcataa attttattat agtcatagtc aaatttatag tgtgctaacc
    14761 atatatcttc tatttcccaa tcatatatag gagatgccgt ccagacattt ttaaattcct
    14821 tagttatcca gcactttcct ttatatccat gctgtttatt cacaatagaa caataacgtt
    14881 gtagagattc tgctgctcgt gttcctaata agcaaacaac ttttgcacct tcatgtatat
    14941 ctctaatcca tctaccaaat tgttttgcca aactttcttg tctcatttta tatttataat
    15001 aagtaaaagg attatttttt tgatttatta tgtattcttt gactggcatt tcccttaccc
    15061 ataactcttc tttctcatca tcccatggat accaatacat ttcataattt cctacagcag
    15121 ttcttgtagc cattggtaaa cacacccaat atggttcaat atcttcaata ttatcttcaa
    15181 atactttttc aacatattca gtagtcaaac tatattgagc ctcaaaatct tgatgaaata
    15241 ctcctatttt ggaacttata ttattctttc tcttataatc caaaactaaa tttagcagta
    15301 aaccactatc ttttcctccg ctaaaagata catagatata atcaaattca ttaaaaagat
    15361 aagaaagtct ttcttgaaaa gcttcaaata catcggattc taaatatctc ttatccattt
    15421 gaattatccc ctttcaatta gaagtttata aaaccttaca atgcaatatt attatgcttt
    15481 tttagcagta atcagcataa attatacatt atttaaatac acaaatccaa ctaactacat
    15541 ataattataa tgtaattata cttttgtatt ataaactata ttttatcatt attttacatt
    15601 tttttccaat atatataaat atcatatttt tctattagct aaaactaatt actttatatt
    15661 tctacgtatt agtataataa tacataaaaa cttgaataat gcataaaaac ttgacatact
    15721 gttgtcataa ttactttgct ataattaaaa gtaaaaatgg aggtaaattc taatgtatga
    15781 acgtatgctt gataaaagca ataaaccctg tttgtctcaa ataatagaat atataggtaa
    15841 agatggatat gcacgattgt gtaagttgga aacttacttg aaaactcaat ataatttatc
    15901 aagagaactt cgatttcctt ttggaaacaa ttatggttgg ggttacaaat atagccataa
    15961 atcttttcat ttattttatg ctttttttga aaaaggtgca tttactgttc tgattcagat
    16021 aggtgacaag caaattccag cagtagaaaa aacgttggta gctttgacac caaaagctaa
    16081 agaactctgg gtcactcgtt atccttgtgg caataatgga ggttggatta agtatcgtat
    16141 tttagaagat gcagacttac ttgatattat tcaatatata agagcaaaaa aatctccacc
    16201 taaacaaaat taacttttat tttttgtgat ttaaatatag tgtatataaa ttccattctt
    16261 tccatataaa aaaaataata taaaaaatat ctattagtat aacttattta gtatgtctta
    16321 ttctactttt tacagataat taaaaagttt tatcataaat aaatcataac tagacatcct
    16381 agtaaatcaa aattaattta ttctttttta ttagaatttt tgataacagt ccctatacat
    16441 atgccaatta tcattccaaa aattagacca taacacattg tgatacctac atttccttta
    16501 gaaataccta tagcacaacc tatagccatt ccaagtatca tgcctataaa catacatata
    16561 ataactaaat tgttgttttt attcatagtt ttctccattc aatatatttt aaattgccta
    16621 gctcgccttc ttagcttaaa acaaagaaat ataaattaac tttctttcat tctaatatct
    16681 gcttgtataa atgtcaaaca acacatttct tgtcataacc tcctcttttt gaagtaagta
    16741 caatctccca gacgcaataa tatcaatttc ctaaaaccat ttaagtaact tttatattta
    16801 cataatattt acaatcataa actatattgt tattcattta tacatcataa acactccctc
    16861 ctaatcatta ttatacttaa aataatgttc atttataata atatatctaa cgcactttaa
    16921 ttgaaaaaac taaaactttg cctttcattc ccatatataa ataagtatac tttttgatgc
    16981 tctattacaa atcaaccaaa ttaattgata taaattatca ttttctgttg aaaataccat
    17041 tcgcacatgc tatacttaat tgcataaatg gtaataaatg tcatatgatt ttacaaatct
    17101 attaataaca attattactt ttactgtatt ttaaattttt tggactaaat atcaagaact
    17161 ttctaaaata caaagtacaa aaaatttata taagttataa atttatatga actagataag
    17221 ctcaaacaat atgttatgta taaactgtgt agtattcggt tataaattta tataaattat
    17281 ataagctaga tatataaatt gcttatataa gctagataag aattatttca actatataaa
    17341 ctacatatat aaattacata cataagctag atatataaac tagatatata aactgcatat
    17401 ataagctata taagaattat ttcaaccata taaactacac atgtaaaatt catgtaaatt
    17461 tactaatata aatcaaataa aattactaaa ctctctaaac taattttgta aaacatactc
    17521 tatttttaac atttgttacc ttatacataa gtacaaacaa aatctaacta aatgaaagga
    17581 taatgtaaaa tgaatatttt aaaaatgaaa aaaacaggga tatttttagg aggagtactt
    17641 tttggtactg taggtgttaa gatactttca agtgattgtg ttaaaaaaac ttgcactcgt
    17701 gcaacagcag gagcattgaa agttaaagat tctgttcttg aaacaactac gaaaattcaa
    17761 gaaaatgtag aagatatttt agctgaagca aaagaaatca acgagcaaga agacttatct
    17821 acatctaagg taaacgttgt acaagatact cttaaatgcg aacaagaagt tcttaaaact
    17881 gaaactataa aaactgtaga agatactaat tctaatagca ttaaatgtga agaaatcaaa
    17941 gataacgtaa ttgaagaaaa aaatagtact tgtacaacag aataaattta atgattatta
    18001 tgattaataa tatcaggagg aagtaaattg aatttttata taaaacacga aataagaggt
    18061 cgtatacgct ttgacttaca acttggcaaa ttaaacaaca agcagtccga tactctctta
    18121 tattatttaa cttctataaa tggtattacc aaagcaaaag tttacgatag aactggtgat
    18181 gcagtcattt attacactgg tgaaagagaa tacataataa gagaaatcac taaattcagt
    18241 tttaacaata gcaaaataga aaacctagtt cctgaacata ccagcaggga attaaacaat
    18301 tattatagag acaaattagc aaataaactg ctagtcaaaa gtatctccaa aatatttttt
    18361 ccaaagtcct taagattttt tttaattgga cttcattcta taaaatattt taaaaaaggt
    18421 ctctcttccc tgctaaataa aaaaatagaa gtttctgttt tagatgccac tgctataggt
    18481 atttctattt ttagaaaaga tatcaatacg gccggttcag ttatgtttct tcttggtatc
    18541 ggtgaactat tagaagaatg gactagaaaa aaatctatag acgacttggc tcaaagtatg
    18601 tcactaaaca ttgaaaaagt atggctaaga aaaggtgaca ctgaaatctt aattccaatt
    18661 tcagagataa aggaaggaga tttagtaagt gtaactatgg gaaatatgat tccattggat
    18721 ggagttatag tatcaggaga aacaatggtc aatcaggcat cacttacagg tgaatccctt
    18781 gcagttaaca aaaaagaaga ctcttacata tatgctggaa ctgtaataga gcaaggcaat
    18841 attgttatgt gcgtcaagga aaaagctggt actactagat tccagaaaat agtaactatg
    18901 attgaggaat ctgaaaaact aaaatcttct gtagagagta aatttgaaca tctagcagat
    18961 acattagttc catacagctt tttaggaagt atattaacct atgcaattac tagaaaccct
    19021 ataaaatcac tctctatatt aatggtagat ttctcttgtg cattaaaact ctctatccct
    19081 atttcagtac tatcagccat gagagaatgt aacaataaca acattactgt aaaaggtgga
    19141 aaatttcttg aaggtgttgc atgtgcagat actattgtat ttgataagac aggcacatta
    19201 acaaaagctc aaccaactgt ttcagatata attacattcc aaaattataa taaggaggat
    19261 atgttaagac ttgcagcttg cttagaagaa cacttcccac actccatagc aaatgcagta
    19321 gtttatgaag ctgaaaaaca agggcttagt cacaaagaaa tgcatacaga agtagaatat
    19381 attattgctc atggcatttc aacaaaagtt gatgataaaa aggttgtaat aggtagcaaa
    19441 cattttgtat ttgaagatga gaagtgtact atcccagacg gtgaggagga taagtacaat
    19501 aatctatcag atgagtattc acacctattt atggcaatat caggtaaatt atcagcagtt
    19561 atttgcataa atgacccttt acgaaaagaa gcaaaatatg taatttcaaa tttaagagaa
    19621 tgcggaataa aaaagattgt gatgatgact ggagatagcg aaaaaacagc taaatctata
    19681 gcaagtaaag ttggtgttga tgaatattat tcagaagtct taccagaaga caaagccaat
    19741 tttgttaaaa gtgaaaaact caaaggtcgc aaagtaatta tgattggtga tggaatcaat
    19801 gattctcctg ctatttcaga atcagatgtc ggaattgcaa tgagtgaagg tgctgaaata
    19861 gcaagagaga tttcagatat taccatatca gcagataatt tgaataattt aatcgtttta
    19921 aagcagatta gtaataagct tatgaaaaga gttgatttaa gttctaagtt tattatcggc
    19981 tttaatttag gtttgatttt acttggtgta ggtggatttg ttagaccaag tacatctgcc
    20041 tttttacaca acgcatctac tgtcggaata agtttaaaca gtatgacaaa tttattggaa
    20101 aacaccaata cttataacta ccattaatct ccattacata aaacatatga tataaaaaat
    20161 ttataattca tcttttaaaa agaatatgtc ttaaaattag cttttttcta atcaaaatta
    20221 tataaagcat actttacaaa ttttaaactc aacttatttc tattttttac aaaaaaggag
    20281 atgattcaaa ttttcatttt gaaacatccc cttttgtaaa tttctttaaa atttatatat
    20341 taattatatt ttcaaccaat aattgtattt tcaaccaatc tttcttcata gcattttaaa
    20401 tcattttgct ttagataatt aaattcagac tctacataaa taccacagcc taccaattca
    20461 attgtcaaat taggtatatt ttcctttagt atactagtct gctcataaat aattctttcc
    20521 aataaattaa cttgtaatat agaaaaatgc ttcataatct ccccatcact atctatatct
    20581 ttcttgattt cataccaact ctttgacaaa attcccttca aatcaatata ccctagactc
    20641 ctcaatatat ctatactatc gctccatgca tacttactag gtttatcaaa gtatgattct
    20701 agtttcttct tctgctcatt tgtaagtgaa tcataagaaa gcttcctatt aaaatgtgcc
    20761 ttcaaagaaa tcttagccat agaatatgga cttctaccag agtttaatcc tgcatttttg
    20821 aacttttcaa ttcctttatc ttctactgaa gaccatattg agtttattga atctgaatca
    20881 ttgtatttaa tatcaagacc atgtgtaata tttatatagt tttgtggaaa tacatattga
    20941 taatagacat cttttttatc atcatataga gagaagtatc cactatcata taaatgaata
    21001 tgcaccttta cttttaaact atcctctaaa atatttcgtc taaggttcgg gatatttgca
    21061 tacatggtta catattttat ggcatcaaag tttttattta atggaacttc aaaactaatt
    21121 tcatctcctg tcaaaagctt tttcacttct tcttcattac aatcatatag agaatatgta
    21181 ttttcaaatc ccacacgtga tttcccatct aaactgcctg ttaatgctac cttcaaaata
    21241 tggttatctg ttttttgcca ttggtaagca tcttggtcaa gaaactctct aatctgtcta
    21301 ttttgctctt tcgcctgatt tataacctcg ttttcatctt ctgtaatccc tacatggcat
    21361 ctatttaaga tatttactat attgcttcca aatgggaatg tccatagttg aaaagctgct
    21421 gcctcaacct gacacaagta tctaacctga tttaattcaa tttgatggat aagttttttc
    21481 tgatattcat tcatctgatt accactatct gacatttgat aaatcaattc tcccattgca
    21541 agattatctt ttttcttatc aatcttatca gtaatgataa tttgagtttc catcatatcc
    21601 tgtacttttt taaatattat agaaatctcc tctgaatttt ccatttttaa gcttccaaac
    21661 tttgtgctta attcagttaa aacttccttc atctttaatt tacttatctc attttcaaaa
    21721 atatcttgat ttttaactgt actatcaaat ttttctacaa atggtataac agattctttc
    21781 aaagttttat ctttaaattc caccatttga gtattcaatt catctaattt cttaatatta
    21841 ttttctttat ctttaattgc ctctttaact acctctattt tactctcaaa actagatttt
    21901 acattaagac aattattaac accactaatc gcagtattta taagagaaca agcagaactt
    21961 ccaatactca atgcattttt aattttagat gtcttttgct gtctttcaat agcttgttta
    22021 cttttctcta gtgcagctaa cttatctttt tgctgtttat tattgtaatt tgcctccaac
    22081 atacctattt cttttttctg gatttttgtg taaatttccg tatactcgct ttcacttaac
    22141 ccctcttttg ctctttgaat atcgtcagct ttgtctgcat aattatcatc atctatagac
    22201 ttaactataa tctccttaat ttggtcaaat ttagttcttc tttcttcatc aacacctgcc
    22261 aaattataag tcattttatc taaagcattt tttctatctt cacttagctg ttctccaagt
    22321 acaaatctca taccatcaga atccaattta aaatctgaaa aatcaaccat cctgttggtc
    22381 cattggtatt cctcttcttt agctgaaaga ttttccttca aagattttgc actgtcaaat
    22441 gcccctagac aagcttgacc aacaccacta aatgcagtca gtaaactcaa tgcgctttgt
    22501 attccagaaa gaattaactg tgttttcatt tgttcctcaa gttttgcctg tttctttttt
    22561 agttcctcta tgtcttcttg ttcatcttct ttcatcttta taatttcttg aatcaacagg
    22621 ttcatttttg cattaacaga aagcatttta ttctcaagtt cattttttaa aacctctatt
    22681 tgttgatttc cagattcaat ttgagttagg atactttcct tatatgtatt gaaatatcct
    22741 tcttttaact catctttctt cccttgttcc aattctctta aggtcttatc cagataatct
    22801 gtcaaagaac gaatatctgt aacacgattt cctccaattt cactttcttc aatcatctgc
    22861 acataaaatg ctgtcttctg ctcaagtgca aataaaatca tactccattc gctaaaattc
    22921 tgattttttc ctacaaaaga ttctattcta tcagactgca tcaatttatt tataaggtat
    22981 tcatagattt cctttgcctc tttataaaat tctttctttg tattcatatc atccagagaa
    23041 agtactgctt tatttttctt ctcaaaacta tagtacttct gaagtatttc tctatttaat
    23101 tgcatctcta ttttctcaac tgcataaaaa atttcataaa tatgtgaaaa ttctaaaata
    23161 tcagcttcta aaattcctac tgcacacaac agtctaagat ttctataacc tttaattcca
    23221 gaaaaaccaa atctctcgaa aaattcatag aagaatgtat ctattgacaa agaatactcc
    23281 tgctttaaca attccttttc ttttagtgca tctccagagc gaccatcttt tccctctgct
    23341 ccggctgaac ctgaatcttc acgtttgcta attatccagt ccttatgttc ttcataaaga
    23401 gcatttaatt tctctccatc atctgctgct tgcttctgat ttaaagcatg attaaacaca
    23461 tatctatcaa aataatagta gtttctatca ctattatcca ttggaggaga ggtacctata
    23521 tatcttattg tttcttcata tatacctcta attccaccaa gacctcctgc accaccagct
    23581 cctccagagc ctccttttcc tgctaaacca tgatttccat cttttttact tatagcactt
    23641 tttaataaag gagcttttcc aaggagataa acctgtccag acttgcttcc aagacctgca
    23701 tttccaccat tacctcctgt gcctccagtt ccaccttgtt ttcctttttc tgcttttacc
    23761 acatagtatt ttgtaccttt aacattagca aattcttttg actctttttt agaacctatt
    23821 ttagcatttg ccccagcttc tccagttatc cctgtatctc ctttttttcc atcttgtcca
    23881 tttccaccac gtccaccatc tacgtaaagt ttaagttctc ctcttttttc tatttcttta
    23941 aaagataata tgatatcacc tgaagtacct gcattattgc catttcttcc attcattccg
    24001 tcgtaaatag aactttctcc aaccaatttt tctgcatcct taccagatac gttgattgaa
    24061 taagtccctt ctggtatatg taatttttct gtaagtagta caatattctt acctgagaag
    24121 acttctttct ccatattaca gtcaattttc aactcacaat ctccatacat atatatagat
    24181 gacaaaatat taagttgata accagatatt attttagtca aatccgataa attcacttta
    24241 ggtataggaa tatatataga aagtacattt tctttctcat atgattttat atatgtacaa
    24301 ctttgaatct tctctatcct atactctgca tcttcaagct ttttggcttc ttcttcccta
    24361 tattcttctt ctgctatcca atatttgata tcttttaatg tatcttctaa agtatttttt
    24421 agcaatgatt ttaacaactc acttagacca acaatatgag tctcactaca gtaactattt
    24481 aactctaaaa gaagacgtgt ttcttctaat atattcactt tctgtacgcc atcaccatat
    24541 gaaagttctt gtacaaactc cataatctct ttcatattat tagtcctact acataaatct
    24601 gtatacatct tatctaattg aatcttttta tcttttagac tatttataga agatagaatg
    24661 gaaatagaat cttccatacc ttttttaaca ctttctatat aaggaactaa aagtttttct
    24721 aggtctttat aggttacatc aacagctttt tgtatttcta ttttacaaga agcagtaagc
    24781 atcaacccca aatctgtttt ttcaaatgtt tcaggtagat ttttcatttc atccaacaac
    24841 ttttctggct gtttatctag tggattacat aatttaatag atacaccatc tgataatgct
    24901 acatttaata agtacactaa ttccttacaa ccgtcgtaat ctttctcttg actatctagg
    24961 tcttcttttg ctttttccaa tatatttctt attttttctt ctgttacatt accaattagt
    25021 gttttaggta ttttattgat aagaaagaag aaattttctt tatactttgc ataatcacca
    25081 agcatatctt tcaaattttt gcctgtttct atcaataatg tatgtctaga gtccagtagg
    25141 gattgatagt ctaaaataac taccatccct tttacctttg aagatgtgga aatagtttcc
    25201 cttacaagca tagcaccagc aatcatatac tctgctccac ggttatcaaa aaaacctggg
    25261 caatcacata atgaaaaatc tctatttgca atatctacaa cttctggaca taaagtctta
    25321 gaatatacac catttccaat ttcgacagct gaatcttttg cagttatata tatttgtcca
    25381 gtctcttcat ccgttttttc ttccatcaca cagcctttta aataatttat acaagtactc
    25441 tttccaacgc ctgaagcacc taagaataac aaaattgact tttcttttaa gtcatatttt
    25501 ctaagctcgt ctcttccttc ttccaaaagg tttacgattt cactataaag tttatttgcc
    25561 atacatccat ccctttctat atttcggaac aatagccttg atatttttct ttaataagat
    25621 tctcttctac ctttgaaaca gctgttgata acttagtgtt tggagcatta aatcctccat
    25681 tgtctcctct atctccatat tcagcgtatt ctcttgctgg tcctgtttta ccttcattcc
    25741 agcttccacc actaaattct atatgatatt taattatact atctaactta taatcccaac
    25801 ttgtaaaata tcctgtatag tatacagtct tatctatacc acttggagaa aatgaaagtg
    25861 tattatatcc gtgatagcca ccacttcctc catatccacc taaacctggt ctaccatttg
    25921 gacctgcttc tccatctttc actgttatat cacaatgatt aattattcta tttttttgtt
    25981 cctctgaatc tgaaatgaag taaatattac ctttcttacc agcatttcca cctcttccag
    26041 catttccacc agctccacct ttttcacctt ttttgccact tgtacctttt tcaaaatacc
    26101 ctttaaacat ctgtccgtgg aaataacttg tatatcctgc atcagctcca ttacttccag
    26161 aagctcctgt ttcaccattt tccccatcta ttcctttaga accatttcct ccatttgcaa
    26221 tcagtgttag acgataattt ctaatttctt ctttaatata taagtaaata ttacctgcat
    26281 ctctaccatc ttcaccattt ttcccataca tagaattatc tacaagttct ccgctctttc
    26341 cacttacatt aatttttaca ttactaccta catcaagatt gttagaagca agaataatat
    26401 ttttctctga tgcaaagttc attccaaggt tatcatccat ataaatagta tctttagcaa
    26461 agaacataaa tgtagaaaca ttgtcatcta cttttctatt tttgaaatat tccaatacac
    26521 tgctaattac taagtttgga attaatgctc ttatttcaat acctttctta tcattaatat
    26581 taattgtttt tactataaat ggatttaaaa tgtatttacc aataaattct ctaattatct
    26641 cattaccttc ttctccatat gcacttaatc ttgttatact gtcttctaac tcttcatttt
    26701 gtgtaactgt aacatttgtc aaaattgaat taaaatcact tacactatat acataatcct
    26761 ttttatttgc aacttccatt aatttttctc tatttggtaa tgtaaatacc tgtttttgca
    26821 cttcataaga aactaaaact ttttctaaat tctttcttat tataatgaag ttttgttcca
    26881 ttatcttctt acttgccttt tctataataa attccatatg tacagaaggt aataaaattt
    26941 ccccagttcc atgttctgta tatttactta aagcatcaat tatatctaag tttcctttta
    27001 tcttattttg taatttcatg caactatctt gtaagaataa tccactttgt ttggctaata
    27061 cgttgataaa tctctctata gtaagacttg ttgtactttc caaccagttt ttaaatactt
    27121 ttacaacctc atcatatttt gctatttctt gaatcattgt tatacttcca ataatattga
    27181 taaaatcttc tgtatacgcc ttaacataat cttctaggct agatacgata ttgttttgaa
    27241 tttcttttat caacgtatct aaaactaatc ttgcatctgc actaattgga tattggaatg
    27301 ctgatgtctt atctttaact tcatctaatt ctagtacttg ctttaataag ccttccctct
    27361 catctgatgc aagtggctta caaagatgaa tattttttcc attatctttt agcaattctt
    27421 caaataacca agctttttcc tctatattac tgtctaaatt ttcgcataat tgttcatatt
    27481 ttctttttat aacattatat atttgttttt ctgttcttcc taagaaactt tttgaaatga
    27541 aaaaagttat actaccctta aaattttctt tatgaagaca catatttaac tgttggaaca
    27601 ggtctagtac ttttgtaagt ctagaatcta atatatctgc ttctggtatc aatacaatta
    27661 ctgcacatat agatgcagaa tttttaaata cttcacctaa tgaaactgct gcactaatat
    27721 catgggctac tcccctattg tcgctaaaac ctgcagtatc acataatttt gcatccattc
    27781 cttctattgg ttttctcata tttacaatgt tcgggtataa agtttgagaa acagctcctg
    27841 aaccaatctc tgccacaggg tctaagcaat caatatatga tataccagta tcttcatctt
    27901 ctacttccat cattacacat cctgcaagat agttaattgc agtacttttg ccacatcctg
    27961 tcccaccaag aattagaatt aaattttgat tttcagcctt atttattgct acttttcctt
    28021 catttaataa gagtgttatt tcttctaaaa gttgattgtt attttctggc atttattttc
    28081 ccccttggtt atatatttta tttaaataaa gtttaatccc ttaagtagat tgcaaagttt
    28141 ttatactata tttaaatttg ttttttattt tttattttta ataaatttgc attttcacta
    28201 aatttgtatt ttcactcaat aattattctt ctttgtttat taaaataact gtgttgtata
    28261 atataatctt tatcaacaca gttattttat ttaatgcgct tattttattg tattttatgt
    28321 aatacagttg ttttttatat tatatgtcca ttattttcct gtggttatta catatagatt
    28381 tttaattata tttgtaccat taatttaaac attcctatac tactgcttaa gttttacacc
    28441 tataccagct tgaatatttt atttttattt gcttaaatag ttcaactata ctggtttaaa
    28501 taggttatct ttatttgctt aaatagttca actatactgg tttaaatagg ttatctttat
    28561 ttgcttaaat agttcaacta tactggttta aataggttat ctttatttgc ttaaataagt
    28621 tcaactatac tggtttaaat aggttatctt tatttgctta aattagttca actatactgg
    28681 tttaaatagt tttcctttag tttaaataat tcacctatac ctacttgaat agttcacttt
    28741 tatttgctta aattctatac caatactggc ttaaacatta tactaccatg attgtattgt
    28801 ctatcttcaa ctacaataaa aacaggttct aaatctttag tagatgttat ttttaattct
    28861 aaaaaaacat tatcagttgg taatattttt acatcataat tatttaaatt attattcgtg
    28921 atactattaa atacatcacc ttgacgttca aaaaaaacat tatcaactat tacaaaataa
    28981 acatcttctt ttctactatt ttcaacctct atagtatttt gtttagttac atttagtctc
    29041 atcttccacc tcctattaat attacttaat tatattaata cttgtaattg ttgcattatg
    29101 ttcatcacaa ggaagaggtt taaaaacaac tatctctgct aaataattct gtggaggatt
    29161 tgtaactgaa gtagcattac aaataaattt aaaatttgtt cctgctacaa cttgagtaga
    29221 aacaataagt ggttcataac caaccccttc taacattcct acagcttcat taaatacttt
    29281 taaatcttct tcttcgacag ctctgaattt tgaccatcca ccaacaaaaa gactcatata
    29341 aaaacctcct ttatatatat atttatttat atatatagta gtaccaattt tctcaatatt
    29401 caatattttc tgtgtaactg gtcaatgaaa atgcataact ggataaaaat acttataaaa
    29461 taacttaact ttacatataa atagcttaat ttacatgtaa taagattatt ctacaatatg
    29521 aaatacatct tattttgaat atattctaaa tacaataatg gatattttca attattatac
    29581 ttagaaaata tccattactt aatttgtttt atttaaaatc acatagagac tttattattt
    29641 tttttattgc atgaatagct atttcaggta tcataaggtg atataccccc tccttgaaac
    29701 cacaaaaaac ataacaaact ttacgaatct tgtcttaaaa gaccacaggt acattcctta
    29761 tttgtggatt ggagtagcca taacaagcaa attttacaat ttttatgatg acaaaaatca
    29821 ataaaatcgc ttagtatata acacgaaata ctacttgttg ctattaagaa tagttagata
    29881 ggaagtatgc attataccaa taaaggaatc aataaagaaa gttaaaacat ccctacaaat
    29941 taaaaatctg ttacattttt ctatatttaa acgttattta cttacaattg gagaaataac
    30001 aggttttcct tctcggtcaa gtaaaggcgt aaatccggct gcatggccat aatagtggaa
    30061 tacatagttc acacctgttt ctttatccac ccaaatctcc gttgcattta tttttccttg
    30121 tgaatatact ttttcaaatc tatcttttcc cattttataa tcctccatat ttttgattgg
    30181 tctatgcctt cttcacttaa gtagaaaaaa tacataactt atttttttaa ttctaatatg
    30241 agcacgtaaa aatgtcaaac agcttattta atttcataac ttcatgttac tgttcgtaac
    30301 cttgtctgtt tacttacact gcattacagc accattttat cctttacatt attgtattgc
    30361 acacagtaaa aatgcttact ttattggatt tttcaactaa aaaaaggatt ttttctccac
    30421 agaacattta ctatttttta taatatatct aattttactc tcatatcaac atttaatgaa
    30481 gatataacct ttttcaaata ttgtaaagac tccttaaaaa tattagaaaa agattctgca
    30541 aatctctcaa catctccttt ttttaaatac ctaagtatct caaaacatac atcaaaacgt
    30601 gttttcaaaa tttcattata ttcttcatga aaaaatctct ctaaatagat tccaaatatc
    30661 atatattgtt ttaaatgtaa aaatgtttcc tgtaatacat aaatttgtgt ataatctact
    30721 atcttacaaa gtaaaacaaa atctggataa accttacact cttcacaatt attttgtaag
    30781 taccttaaat gagtttctat tttatctaca gctatcaaat ccattttatt tcctagtaaa
    30841 agtactacat cctcaagagt aatcgtaaca aactccatag catccaaaag caaactcaac
    30901 cttttagctc cttcttctgt atttatataa tcatcatcat attcagaaaa aagtgttata
    30961 tatgttccct tgccattaat tgttttagca attcccaatt catttagctc attataggca
    31021 cttctaactg ttggaagaga tacattatat ctattaatta tcgaagtaat tgatggtaga
    31081 caatctccta ttttaatatt gccttcaaaa atttgacaaa ttacatctga aacaattaag
    31141 tcatataaat aacatttttc caacgttaca gaattaaaac tacttgtatc tatactttgt
    31201 ggagcacgat tttctaccat tttaagtatg gattctctac aagctttata catatatgtt
    31261 aatagttttt taaattcaat tagattcccg tccaatccat ataagtaagt ttttcttacc
    31321 tgctctatgt atttattacg aaaaaagtct atacaacaat tatccaagtt atccaagtta
    31381 tcgaaattat aggccaattg tgaaataatg acatcatgct gaataatctc acaaaaatgg
    31441 tgaatcagga aattatttgt tttacttatt agtttattta tatattttag cctttgttct
    31501 ttaaagacta aataattaga actatttttt tccatacaat cgataatctc gtttaattct
    31561 tcaaagtctt tagatgtaaa tcttttaata ttgtacatag caatagaagg aaatattaaa
    31621 ctaaaaatct tatatatttc tgatatttgt aaaatcttct catataataa gtcagattct
    31681 attgattgtt tactcgtact ctggattaaa actggtctag aaccagcttt tgtctctata
    31741 aagccctttt ctgagagcat ttttattaca gtttttatag tattttttga taccatatat
    31801 tcttctgaca tgctttttat agaatcaagc ctatctccaa tagtaatttt accagtataa
    31861 atttgttgaa ttatatgctc aaaaataatt aaatattgtg gttttctctt catatttttc
    31921 ctcttcgata aattagattt attgtttttt gtaaggtata tttactttta tttaataggg
    31981 caaaatagtt gcttattttt tcctcataat ctataattaa tatagaccat ttatatatat
    32041 ggtctaagtt ttataatact gtaatgcagt aaaatagcat attgtagcta tactacagca
    32101 aattaacata ccattgtagt atattaattg gaataatttt aagtacttca aaattccagc
    32161 aaaacatata tgtgtttttg atattatgta tattcgttaa tagaatagct atacttgttt
    32221 tacataataa attatatgat acttgctata tgtaattact atatttaata cacataacta
    32281 ttatacttac tatatgtaat tactatattt gatacacata attattatac ttactatata
    32341 taattactat acttggacac ataactatta tacttgctat atgtaattac tatactttat
    32401 acacatagtt attatacatg ctatatataa ttactacact tggacacata actactatat
    32461 ttactatatg taattatact atacgtggac atatagttat catacatact atatgtaatt
    32521 actatacttg gacacataac tattatattt gctatgtgta attatactat acttggacat
    32581 atagttatca tacatactat ataattacta tacttggaca cattatcata catactatat
    32641 gtaattacta tacttataca catagttatc atatatacta tatataatta ctatacttgg
    32701 acacatagtc atcatacatg ctatatgtaa ttactatact tatacacata actattatac
    32761 tttccataca taattactat atttggacac atagttatca tacatactgt atattaagat
    32821 ataatacttt ttaaatttta tataacacag acacatgcaa taaaaaagaa ggtatccata
    32881 ataagctctt tgcatgtacc taaataaaaa ctgtaaaata gaatatataa aatctaatct
    32941 attttaagta aatacaattt cactttaaaa gaggatgtac tatgaaatac catatatttt
    33001 taatcataac tactatatta ataatcgaac tatgtaacat ttacatatgc aaaatagcat
    33061 cttttagaaa caaaaatata actatacata agaattacaa atatattttt ttaataaaac
    33121 cattaacctt cttgataata atattcacaa aattaccatt accactatat tatatattgt
    33181 tttacatagt aagtcataaa ttgctcttaa gcagaaaaag ttctactaac aaccatttta
    33241 ttcccaatat ggcagtaata agtttttgta ttttacacct tattactgta agtacaatat
    33301 cacttgttaa aaatacaagt ccagatataa tatttgcaaa tatgacctat aagttaatta
    33361 gtattttaat agcttctatt ttagatttta tcttatggta ctctatgaat aaaataaaag
    33421 agcatattta tataataact caaaatcaaa tagagttcag tctattttta aaatttatct
    33481 ggttcacttt aggatatata ttatttgata gtatacaaat tacatttgct ccaccaagtt
    33541 ctctaacttc ctactttata ttgggaagta acatattatt aatcttacaa ctaattatat
    33601 tcatcaatca tatatattcc attgaaaaaa atatttattt ggaaaaagaa cataatagac
    33661 taaaaatgat aaaacaagaa caattactaa aagtaaattt attaaagaaa aaagccaaca
    33721 ttgatgaatt aacatcagca tataatcgaa aatttgcttt tgaaaaaata cagcattaca
    33781 ttgataacaa tttattattt tcattgatat atatcgattt ggataaatta aagacgataa
    33841 atgatacata tggacattta gctggggata actatattat taacttctca agtatagtta
    33901 ttgaaaactt aggaatagat gacatatttg ctagaatagg aggagatgag tttatagtta
    33961 ttatgcctaa atctaatgaa aacaccacaa aagaatttat agagaaaata agtaaaatta
    34021 tggataatac gatatctttt agctatggta ttgtagaagt ttctagcgaa aacaaaatga
    34081 ctgtagaaga aattattaat attgctgata ttagaatgta cactaataaa caaaataatc
    34141 ataaaaggaa caataactaa aatgaatatt tacatgagct tacttgtagt ctcactatgt
    34201 tttttatctt attatctcta tcattataat ttttttaaat atctggacat aatgattaat
    34261 aactctaaaa ataaatacac agtatatatt atatattcta tatttaatta tttaattttt
    34321 attatttgta cttatttaaa gtttcatcta attataaatt ggacaatttt tatgttgatt
    34381 ttattcatac aaattaaagt aacttataaa atctccacaa taaaaaacat tttttattca
    34441 ctaaactgct ccatcattgg tttagcaata aatatatttt ttaggtcttt aatgtcaata
    34501 gtgttaaata tgcc
//
LOCUS       DAEMGG010000030        33580 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEMGG010000030 DAEMGG010000000
VERSION     DAEMGG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.030"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            21..890
                     /locus_tag="KPK24_001415"
     CDS             21..890
                     /locus_tag="KPK24_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2299097.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            1116..1982
                     /locus_tag="KPK24_001416"
     CDS             1116..1982
                     /locus_tag="KPK24_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2299098.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            2174..2371
                     /locus_tag="KPK24_001417"
     CDS             2174..2371
                     /locus_tag="KPK24_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2299099.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            2374..2817
                     /locus_tag="KPK24_001418"
     CDS             2374..2817
                     /locus_tag="KPK24_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299100.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(3004..3091)
                     /locus_tag="KPK24_001419"
     tRNA            complement(3004..3091)
                     /locus_tag="KPK24_001419"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3055..3057),aa:Leu,seq:caa)
     gene            3243..5891
                     /gene="polA"
                     /locus_tag="KPK24_001420"
     CDS             3243..5891
                     /gene="polA"
                     /locus_tag="KPK24_001420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG2299101.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            5900..6502
                     /locus_tag="KPK24_001421"
     CDS             5900..6502
                     /locus_tag="KPK24_001421"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG2299102.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            6495..7049
                     /locus_tag="KPK24_001422"
     CDS             6495..7049
                     /locus_tag="KPK24_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG2299103.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            7049..8764
                     /locus_tag="KPK24_001423"
     CDS             7049..8764
                     /locus_tag="KPK24_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2299104.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNITSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            8837..9049
                     /locus_tag="KPK24_001424"
     CDS             8837..9049
                     /locus_tag="KPK24_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG2299105.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            9250..9849
                     /locus_tag="KPK24_001425"
     CDS             9250..9849
                     /locus_tag="KPK24_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299106.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            10077..10448
                     /locus_tag="KPK24_001426"
     CDS             10077..10448
                     /locus_tag="KPK24_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2299107.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            10580..11854
                     /locus_tag="KPK24_001427"
     CDS             10580..11854
                     /locus_tag="KPK24_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2299108.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            11965..12909
                     /locus_tag="KPK24_001428"
     CDS             11965..12909
                     /locus_tag="KPK24_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299109.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            13125..14462
                     /gene="rsxC"
                     /locus_tag="KPK24_001429"
     CDS             13125..14462
                     /gene="rsxC"
                     /locus_tag="KPK24_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG2299110.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            14485..15462
                     /locus_tag="KPK24_001430"
     CDS             14485..15462
                     /locus_tag="KPK24_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG2299111.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADISSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            15462..16031
                     /locus_tag="KPK24_001431"
     CDS             15462..16031
                     /locus_tag="KPK24_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG2299112.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            16044..16637
                     /locus_tag="KPK24_001432"
     CDS             16044..16637
                     /locus_tag="KPK24_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG2299113.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            16652..17227
                     /gene="rsxA"
                     /locus_tag="KPK24_001433"
     CDS             16652..17227
                     /gene="rsxA"
                     /locus_tag="KPK24_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG2299114.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            17243..18214
                     /locus_tag="KPK24_001434"
     CDS             17243..18214
                     /locus_tag="KPK24_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG2299115.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            18434..19018
                     /gene="maf"
                     /locus_tag="KPK24_001435"
     CDS             18434..19018
                     /gene="maf"
                     /locus_tag="KPK24_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG2299116.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            19063..19722
                     /gene="radC"
                     /locus_tag="KPK24_001436"
     CDS             19063..19722
                     /gene="radC"
                     /locus_tag="KPK24_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG2299117.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            19843..20853
                     /locus_tag="KPK24_001437"
     CDS             19843..20853
                     /locus_tag="KPK24_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG2299118.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            20863..21756
                     /gene="mreC"
                     /locus_tag="KPK24_001438"
     CDS             20863..21756
                     /gene="mreC"
                     /locus_tag="KPK24_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG2299119.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            21771..22259
                     /gene="mreD"
                     /locus_tag="KPK24_001439"
     CDS             21771..22259
                     /gene="mreD"
                     /locus_tag="KPK24_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG2299120.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            22271..25249
                     /locus_tag="KPK24_001440"
     CDS             22271..25249
                     /locus_tag="KPK24_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG2299121.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            25347..26030
                     /locus_tag="KPK24_001441"
     CDS             25347..26030
                     /locus_tag="KPK24_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG2299122.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            26054..26851
                     /gene="minD"
                     /locus_tag="KPK24_001442"
     CDS             26054..26851
                     /gene="minD"
                     /locus_tag="KPK24_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG2299123.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            26869..27153
                     /gene="minE"
                     /locus_tag="KPK24_001443"
     CDS             26869..27153
                     /gene="minE"
                     /locus_tag="KPK24_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG2299124.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            27238..28368
                     /gene="rodA"
                     /locus_tag="KPK24_001444"
     CDS             27238..28368
                     /gene="rodA"
                     /locus_tag="KPK24_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG2299125.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            28409..28822
                     /locus_tag="KPK24_001445"
     CDS             28409..28822
                     /locus_tag="KPK24_001445"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG2299126.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            29307..29636
                     /locus_tag="KPK24_001446"
     CDS             29307..29636
                     /locus_tag="KPK24_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2299127.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            29640..30431
                     /locus_tag="KPK24_001447"
     CDS             29640..30431
                     /locus_tag="KPK24_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299128.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            30995..32077
                     /locus_tag="KPK24_001448"
     CDS             30995..32077
                     /locus_tag="KPK24_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG2299129.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(32289..33482)
                     /locus_tag="KPK24_001449"
     CDS             complement(32289..33482)
                     /locus_tag="KPK24_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG2299130.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
BASE COUNT        13181 a         3726 c         6344 g        10329 t
ORIGIN      
        1 agtttgggga ggataaaatt atggattata aaacaatcat attaaataga aagtctgtta
       61 gagaatataa gaacacagat attaaagaag aatatcttag tgaaattaag aaatactcag
      121 aatcttgtaa gaaactagtt ccagaaatag atgttgatgt aagaattatg aatagaagtg
      181 aagtttttaa taatttagat aaagtagcag ggtacaaagg aaatatgata gattctccaa
      241 gttatattat aatattatct gacataaaag ataattacat tgaaaattca gggtacatag
      301 gagaaagtat aatctttaaa gtaacagaac ttggaattgg ttcttgttgg gttacttttg
      361 aagatggaaa aaaggtaaaa gaaaaacttg gaattaattc agataaagag gtaacaggaa
      421 ttatagctat tggatatgga gaaaatgtaa gtaatgctaa agtattaaat gctacaaaaa
      481 ctgggcaaaa ttattcaaaa tctgatatgc aagttgtaac agataagggg tctactagac
      541 taggtgtaga agatgttgtt tatatgaacg aatggggaaa tgttgcaagt atagaaatct
      601 tagaagaaag agcacttctt gatgcatttc actatgcaag attagctcca tctacattaa
      661 atagacaacc atggagattt atagttgatg gaggcactgt agttttagct gttagaaaag
      721 atggtcatac taatttatat gaagaaaaaa tagacattgg tatagttatg ctttattttg
      781 caacaataat aagtgcaact atgtttgatt taaaatggaa tttaggtact ccagacaaag
      841 attataaagt gccagaagac tataagatag taggatattg taatatataa gatagtgtaa
      901 tacttaattt aactacactt aaattaattt ttataaaaag ttcaataaca ttttacaata
      961 taatgttatt gaactttttt attgtataat acaattttta tatttattgt attgattgaa
     1021 aaatgcttat aaatttaaat gcttacaatt ttgtgcatga atagagaggt atattatttt
     1081 attaaactgt ataatttgct tggaggtaat atcaaatgga atggataaat aaattaaatc
     1141 aagctattac ttatattgaa gaaaatctcg aaagtggagt ggattatgca gaagcagcaa
     1201 aaattgcatg ttgttcaacc tttcattttc aaagaatgtt ttcatatatt gcagaagtac
     1261 cattatctga atatattaga cgaagacgta tgactaaggc tgcatttgaa cttcagaaca
     1321 gtaacatcaa aattttagaa ctatctgaga agtatggtta cgattcacca acatctttta
     1381 atagagcttt tcaaaatata cataacattt ctccatctgc agctagagca aagggtgttg
     1441 ttttaaaagc atatcctaag atgacattaa gcatttatgt aaaaggaaat atagaaatgc
     1501 aatatcgtat tgtagaaaag aaagggtttc gtattgttgg tataaaagaa agtatgaaca
     1561 tgattgtaga agagtgcttt gagaaagttc ctaagttgtg ggctaaatgc ataaaaaatg
     1621 gaacaattga taaactttca gagttaataa ataatgaacc ttgtggattg cttggagtta
     1681 gtgtgtgtac aaatagtaag ggtttagatt attatattgc tgctccaaca gataagcctg
     1741 tattagaaga tactcatgaa tacttaatac catcaggcac atgggcagta tttgaatgtg
     1801 tttctccaat gccaaatgct ctggcaatac aggaattgca aaaaagaatt attactgagt
     1861 ggttaccaag ttctgggtat gtgtatgcta atttaccaga tattgaactt tatcccaatg
     1921 gcgatatcaa tgctcctgat tatactacag aagtttggat tccaattcag aaaccaagtt
     1981 aaaattaata aataatatgg aattaataaa taacattaaa attaataaac aacatgaaat
     2041 tctaaaatta caggctgaaa atagtctgtt atttttttgt aaatatggaa agtaagcttt
     2101 acataaatgg aatatagttg tataattatg gaaagtagac tttccgtagt tgagatatta
     2161 aagggggata gatgtgaaaa atagattaga agaaataaga aaagaaagag gcataaaaca
     2221 agaagaactt gcttctattt tgcaagtatc aagacaaact ataggttcat tagaaaatgg
     2281 taggtataat ccttctatca tcttagcctt taagatagct agatactttg atatgagtat
     2341 tgaagaaata tttatttatg aggaggagta aaaatgaaaa agaatatgtt atatgttgga
     2401 attatatatt ttatttgtgg aattttactt gttttattgg ctacatttac tgagttttca
     2461 tttgaggctt ttatttgggg attatctgga gctgctttag gtccaggtgt atgtatgatt
     2521 cttcaatata tgtattggag taaacctgaa agagcagtag attatgaaga aaagataaaa
     2581 aatcaaagga tagagatgaa tgatgaaaga agaattatgt tacgtgataa agctggacgt
     2641 attactaatc aaatcatgtg ctatgttctt gtaattctta tatttattgt ttctatattg
     2701 agtgtatttt ctgtaatggt aataagcaag tgggttttag ttgttcttgg attgcttatt
     2761 ttatttcaat ttatttgtag tgttgttgta tacaataaat tagataaaag gttgtaataa
     2821 gttatgatat aaaatagaaa attatataaa agtaaattag tttttaaatt aatgctagaa
     2881 gataaattta tataattata taaattttaa aaagacacag taatttcaaa aaataactta
     2941 aaatttaaat caaaaataaa aaaactccta atctattgaa taaatagctt taggagtttt
     3001 taatggtgcc cgaggccgga ctcgaaccgg cacggagtat tagtccggtg gattttgagt
     3061 ccactgcgtc taccaattcc accactcggg catgtcatgt atattattat agcaaaaaaa
     3121 tatattgttt gcaacatatt taattttaat atactattat tattatggga atattaaata
     3181 acactatatt ggtatataat atatatgtta caaaaataga taatttttag gagtgatata
     3241 atttggaaaa aaaattaatt ataatagatg gaaattcaat aataaataga gctttttatg
     3301 ctcttccaga aatgaataat aaagaagggt taaagaccaa tgctatatat gggtttacta
     3361 caatgctatt taaaatgata gatatatata aaccaacaca tataagtgta gcttttgaca
     3421 gaaaagcacc tacatttagg catttagaat ataaagatta taaggctgga agaaagggta
     3481 tgccagatga attggcagaa caattacagc cattaaaaga ccttttagat aaatttagaa
     3541 taaatagact agaaatagat ggttatgagg cagatgatat tataggtact gtttctaaaa
     3601 aagcagaaga aaatggatat aaggtataca tagtaactgg agataaagat gctattcaac
     3661 ttgcttcaga taatacaact acccttataa cgaaaaaagg ggttggagaa gtagaagaat
     3721 acaactttaa ttctgtaatt gaaaaatatg agatgactcc aacacaattt atcgatttaa
     3781 agggtcttat gggagacaag tctgataata tacctggagt acctggaata ggtgaaaaaa
     3841 caggtataaa gcttataaaa gagttttcta gtatagagaa tctaatagag aatacagata
     3901 agcttaaagg tagtgtaaaa aagaaaatag aggaaaacaa agaaatagct attcaaagta
     3961 aaagacttgc aacaattata agagatgtgc caatagaagt ggatttagat agtatgattt
     4021 ttggcgatta tgataaagat gaacttttag ataaatttag atactttgga tttacaagtc
     4081 tcttatcaag attagttgat ttggtcgatt cagatgacac agaacacgta gaagaaaaaa
     4141 tagaaatatt aaaattaaaa gatatagaaa aatttataga tgaagtaaac aaaaaaggac
     4201 aagttatttt aaaaactgtt agaggacaag gtaatatact tgaaaaaaat ataatatata
     4261 tttttataag tgtagatgga gaaaaaatat attatgtaga ttcaaatgaa gtgtcaaagt
     4321 tagatagtat attttcaaat aatgaaatta aaaagtatgg atataattta aaagatgatt
     4381 atatatcttt aaaaccatat aatataaact tagaaaatat gtattttgat ataacaatag
     4441 ctgagtactt aatagattca acttcatcta cctcatatga ttgtagtgca atagctatga
     4501 agtatttatc aaaaaaggtt aaatcaaaag aagaattatt aggaaagggt gttaaatcta
     4561 aaaactatga ggatttagaa tttgaggatt tagcagagta tatgggtcaa ataatatgca
     4621 cagttaaaga aacagttcct atgatggaaa gaagtctaat ggatatgagt atggatggtc
     4681 ttttttatca tgtagagatg cctttagtag aagtcttagg tcatatggag tatgaaggta
     4741 taaaagtaga caaaagtata ttggatgaat tgagtgttga atttaaagaa ataatagcta
     4801 cattagaaaa ggaaatttat gaattatcag gagagccatt caatataaat tcaccaaaac
     4861 aattgggtgt tatattattt gaaaaattag agcttccaat aattaaaaaa actaaaactg
     4921 gatattcaac taatgcagat gttttagaaa aattaagaga taagcatcca ataatagata
     4981 agataactga gtatagacaa atagtaaagt taaattctac ttacgttgaa ggacttttaa
     5041 atataataaa tccagaaagt aatagaatac attcatcatt taatcaaact ataacgacta
     5101 caggaagaat ttcatctaca gaaccaaatc ttcaaaatat acctataaaa atggaaatgg
     5161 gtagaaaaat aagaaaagtg tttattgcag atgataactg taagttggtt gatgccgatt
     5221 actcacaagt agagctaaga gttcttgcac atatgagtca ggatgaaaat atgatagaag
     5281 catttaaaca tcatgaagat attcatacca aaacagcttc acaagttttt aatgttccta
     5341 tggaagatgt tactagtgaa ttgagaggtg cagctaaagc tgtaaacttt ggtattatat
     5401 atggtaaaag tgactttggt ctagctgatg atttaaatat acctgttcca aaggcgaaag
     5461 aatatatcga aagttatttt gctaaatacc ataaaataaa agagtttatg gacacaacag
     5521 tagaaaaggc tagtgaggat ggatatgctg taacaatttt aaacagaaga agatatatac
     5581 cagaaataaa gtcaagcaat tttatggaaa gaaatagagg aaagagattt gcaatgaatg
     5641 caccaataca aggaagtgca gcagatataa taaaaattgc tatgataaat gttcataaaa
     5701 aattggaaga gaacaatttg aaatcaaaac ttatactaca agttcacgac gagttgatag
     5761 ttgaagcaat tgatgatgaa atagatatag ttaagaaaat agtaaaagaa gaaatggaaa
     5821 atgctgtaaa tatggatgtt cacttagatg tagatttaaa tgtaggtagt tcttggtatg
     5881 agacaaagta ggtgatttta tgttgatatt aggacttaca ggaggaatag gctgtggaaa
     5941 aagcagccta tcaaatatat ttagaaatct taacattcct atagtggatg ctgatattat
     6001 atccaggaag atatttgagg ataagttgtt attggaaaaa gtatttgtcc attttgggca
     6061 aagtataaaa aatgatgatg gaactctaaa tagaaaagct cttgggaaaa tagtttttag
     6121 tgatgaagaa aaacttaagg aattaaataa cttgactcat ccacgaataa gagaaaaaat
     6181 aataagtgaa atagaaaaat tgagaaaaaa aggcgaaaat atagtagtgt tagatgcggc
     6241 aatattggta gaaagtggtt ttttagatat ggttgacaag ctattagtag ttacttgtaa
     6301 acaagaagta caaattagta gaatacaaaa gagagataat tgcagtgagc aggaagcact
     6361 tagtaggata aattcacaga tgagtcagga agaaaaatcc aaatatggtg attatataat
     6421 agataattcg ggaacaataa ctgaattaga aagtaaagca cataaattta ttgagtacat
     6481 gaaggagaat tggcgtgaat agtaagaaag tattgattct atctattttt ataatcttat
     6541 ttggggcact attaatggaa agcaaagtaa tacataaatt tttatatcct aaaaaatatt
     6601 cagagtatgt agaaaagtat tcgaaagaat ttaatttaga tgaaaatata gtttacagtg
     6661 ttattaaagc cgaaagtaag tttaatagtt ctgctgtttc aaaaaaggaa gcaaaaggat
     6721 taatgcaaat attagacata actagagatt ggggagcaga ggaactaaat ttaaaaaatg
     6781 tggatatttt cgacccagag actaatataa gacttggctg ttggtattta agtaagttat
     6841 acaaagaatt tggtaaatta gatttagtga tagctgcata taatggtggt tcaggtaatg
     6901 tgaaaaaatg gttagaaaat aatgaatata gtaaagatgg cgaaaatcta catgatatac
     6961 cttttaagca aacttcaaaa tatgtagaaa aagtaaaaaa taattacgaa cattataata
     7021 agatatatgg caagaaagga aaaaactaat gaagagaata aaagtcttaa cagtcgtttt
     7081 agctataact cttatggtag ctggttgtag taatactaaa acaaaacaaa gtagtagtga
     7141 ttcaagttta caaagtcaaa gttctataga tgctaagtat ataaacttaa caatggtaac
     7201 tccaaaaact ataaatccta taactaatac tgataagtct gttggttata taatgaattt
     7261 agtttatgat agtttattta ctatagatga gaactacaat gtaattcctc aattagttaa
     7321 ggagtataat atagctcagg atggaatgag tatagatata aagctaaaag atgcaaagtg
     7381 gcatgatgga aaaaatatta cttcaaatga tgtaaactat actattggtt taattcaaaa
     7441 aagtgcagat agtccatata atgaatttac taaaaatata gcttctgtga atattaaatc
     7501 agataaagat tttacaataa aatttaaagc tagatatgct ttttcaattg atagtttaat
     7561 ttttccaatt gtatctcaaa atcatttaga ttcaaaagat gtaaatgaca agaataataa
     7621 tatgatagga aatgggaaat ataaaataga atcatataca gaaagagaag gaatggtttt
     7681 atctgtaaac aaagattact atgaagaagt tccaaaaacc atgaagaaca taaaagtagg
     7741 tatggtgcct aatgaagatg caaggacatc tatggttatg gctcttgata gtgatataac
     7801 caatgttaca ttaaatgatt taagtaagtt tcaagaaaaa gagtttaata taacaaaata
     7861 tcaaggaaga gattatgagt gtgtattatt taattataat aatccttttt tcaaagatgt
     7921 taattttaga aaagcaattg cacactctat agataaagat agaattatta gtgaaggtta
     7981 tatggatgat gctactccag ttaattttcc acttaattca aaatcaaaat actataatag
     8041 tgaaatgaag gatttagagt ttaataaaga taaggcgata gagtgtcttg ctaaggtaga
     8101 atatgcaaat gtaaactcag taaatcaaaa tgacaataaa gtaaaaaatc agaaaaaatc
     8161 agctaaaaag aactcaaaga agcttacgcc agaagaagaa gcaaaagcta aaaaagcaga
     8221 agaagataga atcaaaaaag aagctgaaga gaaaaaaaat agagaaaaag aggaagtaaa
     8281 gaaaagtctt tctgaaatga acttaaagat aatagtaaac agagataata gtgagagaat
     8341 aaaaactgca aacataataa atgaaaactt aaaagctata ggaataaata gtacagtaaa
     8401 tcagctgtca gataaggata tggaaaatgc tcttaattct aaaaattatg atttagcatt
     8461 agttggatgg aaactatcaa ttataccaga tgcaagtagt attatagcga gtagtggata
     8521 tactgatgat aagttaaatg gatatatgtc tgcacttaca agttctacgt ctgaaattga
     8581 aacaaaaaaa gcatacaaag atgtacagac atatataaaa gataatgcaa catttataag
     8641 tttagctgtg agaaacaact atattgtaag taatagtaga ttaaaaggta aaattactcc
     8701 aaatgatttt gatgtatatg agggaatatc taatttagat ataaaatcga atgaatcaaa
     8761 ttaaatgatt aatttctatt taaaaagtat aaactatatg atagaatacc cttatagggt
     8821 ataaggaggt aattaaatga agaagaaact attagtagaa ggtatgagtt gtggtcattg
     8881 tgtaaaccat cttaaaactg cattaacaga agatatagat ggtattgaag ttttagatgt
     8941 agatttagaa aataagtgtg catctgtaga tatgaaagat gatgtatcta ttgaaaaatt
     9001 aaaagaagtg atagcagaat taggatttga actaaaggga attgaataga aattgataaa
     9061 ctaaaaaaag acctctattg ggggagtaat agaggtctaa aaatggggga gattgagtat
     9121 gtttgatttt acattactat tatctcccct tttaaattaa tatatacata aaaagtaaaa
     9181 aaattttgta atgactcaca tttcttgagc tttttgaata tactataaat agctaaggga
     9241 gggagtatca tggaatctag gggatataaa gaggatttaa aaaaaatgat acttgatatg
     9301 gcccacaaag aacttgagag ttatgtatca caaaaaggag caaaaaaata ctttgaaggt
     9361 aacttggata agattattaa taataatata aaaaaattca atcaaagtat tggtcttaat
     9421 agagtatatt taaaaatgtt aaaaaagtgt gcatatcaag aaagtgtaac ttcaatatca
     9481 agaactataa agaaagaagc attatttaaa tcaaaaaaag agctatttgc tttttcagag
     9541 tacttaaaac ttgatattaa caagcgactt tcatataatc aaattttaag aaaaatatca
     9601 agatatatat actacaatag aagttcatat gctcaaaaat acgttgtttt caaaagagga
     9661 gatgaagaat atcttttaga accagagaaa ataaaagatg aattaatatc aagctatagg
     9721 tcaaagacta gagaagatat gaggtcaata gcaaaacttt tggatataga agtagaagat
     9781 gattataatg ctgaagatat tagaaaaaaa gtaataaatt atattattaa agaaaaactt
     9841 aaaagttaga gttttaaaga ataagttctg ccaatttgca tgattaagta taacttgggt
     9901 ataaattttt tatgcatttg aaagagctta ttcttttttt gacatattct tttaaataat
     9961 ttaccatgat ttggtaattg gtcatcatca ttcaaatttt taacttaata tgatttaaat
    10021 aaatttataa taatgatata atataaaaaa aataaaaatt ggagagtgat ttcaaaatga
    10081 agtttaattt ttgtcataac aatttcaatg ttactaattt agaaaaaagt ttagattttt
    10141 ataaaaaagc tttagggctt aaagaggtaa agcgcaagga agcagaagat ggaagtttta
    10201 tattagttta tttaggagat ggcattacta gtcatacatt agaattaact tggcttagag
    10261 attgggatag accatataat ttaggagaca atgaatttca tctggcttta gaagttgagg
    10321 agtttgatga ggcaaaaaaa cttcataaag atttagattg catatgtttt gaaaatgaaa
    10381 gtatgggaat ttattttata gcggacccag ataattattg gattgaaata cttcctaaaa
    10441 atcactaatt ttaaggatat gtttataatt tctttacaat taataagaaa ataagttatc
    10501 attaaattgt agggactaaa aatggataat aatgttatga atttatgact ataaaaattt
    10561 gttgggggga gtgatagtag tgaaaaaagc tatagcagct ttaggaattg gggcagtagc
    10621 agtatctgtt agctccataa atgcaagtgc tcttgaaaag ggaacagtaa cagcaagtgc
    10681 cttgaatata agaagtggac caagttctga ttgcgataag gtagcaaaat tatacaaggg
    10741 aaaaactgta gagattttag aaaagtctaa tggatggtat aaagtaaggg tatcgagttc
    10801 tgttgttgga tggggaagtg caaaatacat atcaacaagt ggttcgtctg aaggtacatc
    10861 aagtcaaaat aatccaactt caagtgggac aactattagt ggaaatggta aagtaaatgt
    10921 gagttctaga ctgaatgtaa gaagtggtgc aggtactaac tattcattag ttggtaaagc
    10981 aaataatgga gatgtagtaa agttactaga acaaagcaat ggatggtata aaataaaact
    11041 atcaaatgga gttactggat gggctagtag ccaatatatt tctaaaacat cagaagatgt
    11101 tggaacgaac aattctagta attcaaatag tactaataat tctgataaaa aaccatctag
    11161 cgaagaatca attgaaggca agaatggtaa ggtaacatct gctgtgagtt taaatgtaag
    11221 aagtggtcct gggacaagct attctataat aggaaaatta aatggtggag atgttgttga
    11281 attaaaagct aaaagtaatg gttggtataa ggtaaagtta tcgagtggaa caattggatg
    11341 ggtgagtgcc agttatattt ctgagactaa tgaggataca aaagaaaaac caaattcatc
    11401 atctaaccaa aattctcaat ctaatagtaa ctcaaagcca tcatttactg gaaattcaga
    11461 taaatctaca gcaaaaggtt caacaatcgt agattttgca tatactctta ttggaatacc
    11521 atatcaatgg ggagcttcag gacctgataa atttgactgt tctggattta cacagtatgt
    11581 ttttaaacat tctgtaggcg tttctattcc tagagtttct agagaacagg ctaattttgg
    11641 aagtgcaatt tctatgggaa attatgcacc aggagattta gtttattttg acactgatgg
    11701 tgatggaaca actaatcatg taggtatata cgtaggaaat agtaaattta tccattgtag
    11761 tggtactcag actaatccta ataaggttaa agtggataat ttaactagtt catattggtc
    11821 taaagtatta cttggtgcaa gaagatttgt ttagtaagta caatttctta ttaaaaaatt
    11881 agaagaaata attatttata ttcataaatt gtgactctta aaaatatact ttattaataa
    11941 tataattttg ggagggacaa actaatgaat agaagaaaaa cgatatacag aggatttaat
    12001 aataatagac ataagattga tttacgtaaa tatgttataa cgattgcttg cttgtgtcta
    12061 attggctatt attcttatac aaaaataaaa gatagcaaga tattggagta tgtatcggct
    12121 aaaataccat ttctaaataa ttcatcagat ataacttata aagatatatc tgatgaatta
    12181 aattcaataa aaaaagggaa aaaatctaaa tcacaaacca attcagatga taaacaagaa
    12241 actaatccag aaaaagcggt aaataacact aaagagccag aagaagtcaa attagctact
    12301 atagaaggct gggatatgta tactattcaa gttgctgcaa tagataataa tgatgatttg
    12361 aaaaaaatac agacatcatt agttaataat gacatacctt tttcagttat ggagaaggat
    12421 ggagtaaaaa aaatacagac ctattcctct tttgatgaaa atgatgttag aaagcagata
    12481 agcagtgtaa gaaaggtgtt tccagatgca tttttatcac acttagatgc acctatgttg
    12541 tctttagagt atacaagtaa ctacgcttat atagagagta tatctaaaga attaaataaa
    12601 ttaataacaa actttaaaga agaatcaagt ttttggtcga atgctgaaaa taatgtagat
    12661 atggaaaaat ataatactat tttgacaaat agaaaggcaa tatcacaaaa tatatcaaaa
    12721 gaagctgaaa agattgatta ttctgaaatg cgtttattta aagataattt aatagagtat
    12781 gtaaagaatg tgaatgaaaa aattgataca gcttcaaaag cagcaaatga agaaaaatat
    12841 agtgtaagta aaagtctgct tttatcatcc atgcaggagt actctatgtt tataaattct
    12901 ataaaataaa atacagaaaa tttggacttt agaaagctat atgaaaagga ttgtttaatt
    12961 aagcaatcct tttcctcaat ttgacttaat atagaggtgt tgatttataa aataaattat
    13021 aaaaatacgg atattttttt aacaattgtt aagatttttt aatatatata gtatacttat
    13081 agaaggcaga aaaaatgttt ttttatagat aagggggtaa aacaatgaaa ctcttaactt
    13141 ttaagggagg gatacatcct ccatatagaa aagagtactc taacacaaag gcacttgaaa
    13201 aagctcaagc tccaaagata gtttacattc ctcttcagca acatataggg gcaccagcta
    13261 aacctattgt tgaagttggt gatgaagtaa agtttgggca aaaaataggt gaacaacaag
    13321 gttttgtttc atgtaacgtt cattcttcag tatcaggtaa agttattgct attgaacaac
    13381 atgaagtacc aggaggttca gctcaatgtg tagttatcga aaatgacttt aaggaagaat
    13441 tacatgagag tgttcaacca aaaggtcagt tagaggattt gagcaaagaa gatatagtag
    13501 gtatcataaa agaggcagga atagtaggta tgggtggagc tacattccct aaccatgtaa
    13561 aagtatctcc tccaccagat agcaaggcag aagtagttat attaaatggt gcagaatgtg
    13621 aaccatactt aactgcagac cacagattaa tggttgaaaa tccagaagat gttgtgtttg
    13681 gtttaagagc attaatgaag gtattagatg tcaaaaaagg atttatagga attgaaacga
    13741 ataaaccaga tgctatagaa gcaattcaaa atgtagctaa agactatagc gaaatcgaag
    13801 ttgttggcct tcaagtgaaa tatccacaag gagcagagaa acaacttatt tatgcgtgta
    13861 caggcaaaga agttccatct ggtggattac caatagcagc aggtgcagtt gttgacaacg
    13921 ttgccacagc agcacagata gctaagtcta ttaaaacagg tatgcctttg gttgaaagaa
    13981 tcactacaat aacaggtagt tgcatcaaag agccaaagaa cctaataaca aaagttggaa
    14041 cattagtttc agaaataata gaacaatgtg gtggatttaa agaagataag aagattggta
    14101 aggttataat gggtggacct atgatgggaa tagctcaata tactactgaa atagcgacta
    14161 acaaaggttc ttcaggaatt ttatgtttgg atgaggaaga atcacgtact ccagatattc
    14221 aaaactgttt aagatgtgga agatgtacag atgtatgtcc atcattctta caaccacttt
    14281 tcataagtgc atattcttta aaagatgact atgacacagc tgaatatcat agagcaatgg
    14341 attgtataga atgtgggtct tgctcattta tttgtccagc aagaagacca ttacttcaat
    14401 ctataagatc agcaaagaga gaaataggag caaagagaag aaagcaagct gctcagaaat
    14461 aaagaaagtt aagggggaaa aacgatggaa aataagttga tagtatcatc ttctcctcat
    14521 gtgagaagta atgaagatac ttcatatata atgaaacaag ttattatagc actccttcca
    14581 gcagcagtag caggagtata cttctttaga cttaatgctt tgagtgctat gtttttttgt
    14641 atacttggta cagtaggtac tgaattttta tatcaaaaat ttacgaagca aaaaagtact
    14701 ataggagatt tttcagctgt tgtaacagga ttattattag catttaacgt accagcatca
    14761 cttccttggt ggatgtgtct agtaggagga atatttgcaa tattagtagt taaaatggta
    14821 tttggtggaa ttggatgtaa ctttgtcaat ccagcacttg ctgcaagagc atttttatta
    14881 gcatcattcc cagtagcaat gactgcttgg actcaaccag gtgttaactg gataggtaaa
    14941 aatttagatg cagttactac agcaactcca ttaagctttt tgaaaaatgg agcagcagga
    15001 ctggcagata tttctagcaa tggaattagt cttgctgaca tgatgatagg gaatattggt
    15061 ggatgtatag gtgagacatc agcaatatta ttattattag gtggagtata cctaatgtat
    15121 aaaggcataa taaattatgt tataccagta ttttacatag caactgtatt tatattaaca
    15181 ttccttttag gtggatttaa tataactttc gcaatatatc aactgtttgc tggaggactt
    15241 atgttaggtg gattcttcat gcttacggat tatacaactt cgcctatgac taaaaaaggt
    15301 caaataatat acgctgtatt agctggtctt ataacaactg ttataagaat gtatggtgga
    15361 tatccagaag gtgtatctta ctcaatacta ttagtgaact gtcttgctcc acttatagat
    15421 aagtttgtta gaaatagagt atttggggag gtggctaaat aatgaacagt atggtgagat
    15481 taggtggtac tttacttgct ataagtgcaa tagctgcatt agctcttgga gctactaatc
    15541 aggttacagc tccagtaata gaacaaagaa atatacaagc aaataatgaa cttagaaaag
    15601 cagtccttcc agaagcaaaa gaatttaaag aaatgaagga agatacttac aaaggccttg
    15661 gagatggtct tatagctgaa gtgtatgaag gtttagatgg ttctgaagtg gttggatata
    15721 ctttaaaagc taaaccaagt ggatatggtg gagaaattga agttatggta ggtatatcat
    15781 ctgaaggtca agttacaggt gttgacattg gtaatatgtc agaaacagca ggtcttggag
    15841 caaaagctaa ggatgatgca tttaagggtc aatacaaagg aaaaactgct gagcctcttg
    15901 aagtagcaaa aggaagtaca acagcagata atcaaatatt agcaatatca ggtgcaacta
    15961 ttacatctac agcagttact acaggagtaa atgctgctat agatgttttc aatagtgctc
    16021 ttaataaata aggagggtta attatgaatt tagctaaagt atttaaaaat gggctaatag
    16081 atgagaaccc aacatttgtt caagttatag gtatgtgtcc aacactagcc gttacaactt
    16141 cagcaataaa tggaattggt atgggtcttt caacagcagc agttttaata tgtgcaaatt
    16201 tagttatatc tttaataaga aaaattacac cagataagat aagaatacct atatttatag
    16261 tagttatagc tacatttgtt acaatagtag gtatgctttt aaaagcatat gttcctgctc
    16321 ttgataaggc tcttggtata tatataccat taatagttgt taactgttta atattagctc
    16381 gtgcagaaag ttttgctttc aaaacaggag ctatgccttc aatagttgat ggtgtaggtc
    16441 aaggtcttgg atttactgtt gcacttacaa taataggtgc agtaagagaa ttacttggaa
    16501 atggtagttt atttggaatg acattatttg gagcatcttt ccaaccagta ttaatattta
    16561 tattaccacc aggtgcattc ttaacattag gattcttatt tgcaggattt aataaattaa
    16621 gaagtaaaaa agcatagtgg aggtgtaata aatgaattta atacttttat ttttaagtat
    16681 cgttcttgtt aataacgtta taacatctca attcttgggt atatgtcctt tcttgggagt
    16741 ttctaagaag gttgatacag cagtagggat gggagttgca gttacgttcg ttttaactct
    16801 agcttcaatt ataacttatt ttatacagat tttattagta aaaactggaa ctggattctt
    16861 acaaacaata gcttttatat tggttatagc ttctatagtt cagttcgttg aaatggtaat
    16921 tcaaaagatg agtccatctt tatatcaagc attaggagtt tatttacctc ttataactac
    16981 aaactgtgca gttcttggta tagctctagt taatgttcaa aaaagttata atttagtaga
    17041 aacaataata aacggatttg gagccggagc tggatttact ttagcaatag taatattcgc
    17101 aggtataaga gaaagactag aattagctga tataccagaa gcatttaaag gtttccctat
    17161 aacgcttata tcagcaggtt taatgtcaat agctttctta ggatttacag gacttataaa
    17221 gctataaaaa agaggggtga aaatggtgat acttacagct gtattagttt taggaattat
    17281 gggcttaatc ttcgggatag tactagattt tgcatctaaa aagtttgctg tagaagtaga
    17341 cgagagagtg gaagctatac taggagtact tccaggagcc aactgtggag gttgtggatt
    17401 tccaggttgt ggaggtcttg caaatgcgat agttgaaggc aatgcaccag ttaatggttg
    17461 ccctgtaggt ggagcagatg ttggtgcaaa agttggagaa ataatgggaa taagtgctga
    17521 agcaggtgag aaacaagtag caaaagttat ttgtaaagga acttgttcaa gtgcaaaaga
    17581 taaatatgag tatgaaggaa tatcagattg tagagcagca aatgttttaa actcaggtgc
    17641 taagatgtgt aaatttggat gtttaggtct tggaacatgt aaagatgctt gtaaatttga
    17701 tgctatttct atagttgatg gaatagcagt aatagatgaa gaaaagtgtg taaattgtgg
    17761 aaaatgtaaa gaagtttgtc ctaagggtat aataatcaca aaaccagaaa gtcaagaagt
    17821 agttgtagag tgtaatagca aagagtttgg taaagctgtt aaagaaaaat gtactgctgg
    17881 atgtattggc tgtggaatgt gtgtaaaagc ttgtaaattt gatgcaataa tatttgaaga
    17941 caaaatagct aagatagacc ctaataagtg tgtaggttgt atgcaatgtg tagcaaaatg
    18001 ccctacaaaa gtaatatcag gtgacataac taagaaaaag aaagttacta ttgaccaaga
    18061 actttgtgtt ggatgtacag tgtgcaaaaa acaatgtaag tttgatgcaa tagagggaga
    18121 attaaaagaa aaacataagg tagatgcaga taaatgtgta ggatgccatt tatgtatgga
    18181 aaaatgtcca aagaaagcta ttaaaatttt gtaatatgga caaaataagg atatggaata
    18241 atttccatat ctctaatgag tacataaaac agaggtatgg aataatttcc atacctctat
    18301 tttatgtata tttattctaa tggtaataaa tataacaata gtgtaacaaa aaaggttcga
    18361 catttctgtt gaatttttta caatatagtg atagactaaa taggtacaaa ttacgtacaa
    18421 atgaggtgaa taaatgaata taatcttggc atcagcctct ccaagaagga aagaaattct
    18481 agagaatacg aatgtaagat ttgatataat taaaaatgaa attgatgaga ttatattgga
    18541 aggtgaaaca ccaaaacatt tagttatgag attagctttt gaaaaaagta tgtctgtagc
    18601 atctgaacat aatgaagatg ttgttattgg agctgataca gttgttgttt tagacaatgc
    18661 tattttagga aaaccaaaag atgagtcttg tgcaagagat atgttaaaaa gactatctgg
    18721 aagagagcat caagtcataa caggtattag cttaataaac ttgtgcgaag ataaaaaggt
    18781 aattgactat gtaatcagca atgtgaaatt taaaacgtta tcagagcagg atattgagga
    18841 ttatctgaaa actaatgagt catttgacaa agcaggagcc tatggtatac agggttatgg
    18901 agctttgtta gttgaagaaa ttagaggaga ttattttaat atagttggtc ttccaatttc
    18961 aaggttaggc gacctattaa aaaaatattt tagtataaat cttttttatg gagtgtgatt
    19021 tatctgaaaa agtcatttaa ttcaaccatt aaggtaaaag aaatggcacc agaggaaaga
    19081 cccagagaaa agatgcttgc caaaggcgta aaaagcttat caaatgctga gttgttggct
    19141 atacttttaa gaactggcaa caagaataaa aatgctattg aattagccaa ctatataata
    19201 aatagagata ttcagggtat tagacattta gaagatatga caatagagga gctgtgtaat
    19261 atagatggaa ttgggttatc aaaatcaacc caaattaaag cagctttaga actaggctct
    19321 agagtagcta gttttaaacc tataaagtat aaaataagga atccttggga tatacagagg
    19381 tattatatgg atagcctaag gtatttaaaa aaagaggttt ttaaagcggt tcttttaaac
    19441 acaaaaaatg agataatatc tgatgtagat gtatctatag ggactttaag ctcgtcatta
    19501 gtccatccaa gagaggtttt taaagaagct ataagaagaa gtgcaagtaa gataatagtg
    19561 atgcataatc acccttcagg aagtgtagaa ccatcaagag aagataaaaa tatcacatca
    19621 agacttatta aatgtggaga aataattgga atagaaataa tagaccatat aattattgga
    19681 gatggattat actttagctt taaagaaaat atgataattt gactttatat gtgataaaat
    19741 aataggagaa tgttttaaac atgattctta atagtaggaa ggagtaaaat catggctaag
    19801 gaaaaaaaga aagaaaaaaa aggttttttt agttttaata agatgacaaa ggatatggga
    19861 atagatttag ggactgcaaa tacattagtt tacataaaag gacaaggtat tgttgttaga
    19921 gaaccatcag ttgtcgcaat aagagatgat agtaaagagg tattagcagt aggtgaagaa
    19981 gccaaaaaaa tgatagggag aacaccagga aatatagtag ctataagacc tatgaaagat
    20041 ggagtaatag ctgattttga cataactcaa tcaatgatta gctattttat acaaaaagcg
    20101 gctgataaaa aaggtgttgt aagtccaaga atagctatat gtgttccttt tggagttaca
    20161 gaagtagaaa agagagctat agaagaggct gcaagacaag caggagctaa agatgcattc
    20221 cttatagaag agccaatggc agcggctata ggggctggat taaaagttga agaaccagaa
    20281 ggtaatatgg tagttgatat tggtggaggt acttcagaaa tagctgtaat atctttagga
    20341 ggaatcgtta ctgctaaatc tataagaata ggtggagacg aatttgacga gtctatagtg
    20401 gcttatgtaa aaaaagaata taatcttatg ataggtgaaa gaactgctga aaatgtaaaa
    20461 attaacatag gttctacatt taaagatgat gaagaaataa atatgcaaat aagaggtaga
    20521 gatttaatct caggattacc taaaacaata gaaatatgct ctacagaagt tagagaagca
    20581 ttaaaagaac ctgtaagttc aatagtagat gcaataaaat ctacactaga aagaactcca
    20641 ccagaattag cctcagatat aatggaaaac ggaataatgc taacaggagg aggcgctttg
    20701 cttagaggtc ttgataagct tattacacag gaaactggta tgtctgtaca aatagcagaa
    20761 acacctcttg attgtgttgc acttggaaca ggtaaatctg ttgaagacca agaaatcttt
    20821 gaaaaagtat taatgatgaa tactaagaat tagaagttgg tgatggcctt gagatttgac
    20881 aagaacaaaa atgagaaaaa aaggattaat gttaaagtga tagcaacggg cgtagttgct
    20941 atcactttaa ttggaattgt gggtatatca attggtaaat tttctagtgg tagtccagta
    21001 aatttaggag tagcttcaga tgctattaca agtgtaggta agggaataaa tgatggattt
    21061 tcttttatca aaaatggttt taaagatgta gcaaatttta aagataattc taaaaaggta
    21121 aaaaaattag aagaagaaaa tgaaaaactt aaaaaaaata tgatagcttt aaatgccaaa
    21181 ctagataaaa cagaatcgct agaagagtta aaaaagacac tgaactttgt acaagaagag
    21241 tataaggcga caagtatttc agcaagtgtt gttggtaaaa atgatggaaa ctggtatgag
    21301 agttttgtca taggtgcagg aaaaaattca ggtgtaaaaa aagaaagtat agttatgaat
    21361 ggtagtggat tagttggcat agtttatgag gtttccaaca attatagtaa ggcaatttca
    21421 ctattggatt ctaaggcatc tgtaagcttt aaactagcaa aggatgccaa tgctaaaggt
    21481 acaattactc aaaatactac tttagataat aaagatagtt ataatagtaa aggatatttg
    21541 caaggatata tgtttgactc atcatataat gtaattcaag gagatataat aacaacatca
    21601 ggtcttggat tcttcccaga tggtatacca attggcgaag ttgaaaaagt ggttgatgat
    21661 aaagataagt cattaaaata tgtagttgta aaaccttatg ttgattttaa gaatataaat
    21721 gacgttgtag tcatagaacc aagaaatata ggataaaggg gataaaaaca atgaaaaaag
    21781 ttttactttg tctgttgggg attgtgctta tgactataga aaatagtata acaaattata
    21841 tagatatttt tggcataagc tttaatttag ttcttatata tgtaaccata atctctcttt
    21901 atttagatga attagaaggt gggattatag cagcaataat agggcttatt aaagacataa
    21961 ccgttggtgg aatttttggt gtaaatgcat taattttgtt tattattgca tatgcaattg
    22021 gatacatgag agatcagcta tataaaggta gttatattat tactttcgtt ttaattttta
    22081 taggcagttt aattgattca attgtcaata taggtacatc tagtataatt tatcaaagct
    22141 acaacatatc aacacttttt gtaaaaggtc tactagtagc accaatagtg aatagtttaa
    22201 ttggtttagt aatatatcgc ttatctaaaa gagcagtttt aaaacttaaa gaagattagt
    22261 tggtgatttc atggaagaaa ataaacaagt tgatagacta aaaataatta agatagtcat
    22321 gggcgtgata tttttagcta ttttagtaaa aataatctat atgactacat ttaaatatga
    22381 atattacaat gagttagctg agaataaaac atataaaaag ttggcaatag aagctccaag
    22441 aggagaaata aaagatagat atggaagact gttggcagga aataaaaact tatttactgt
    22501 tcaagtttca ggtaacgata ttaataagaa agatgcaaat aagcattcaa gagcaaatga
    22561 aatatcatta aaacttataa atttgctgga gagaaatggt gaagaatatg tagatgagtt
    22621 tcctatatat gtagaaaatg gaaagtacta ttatacatat gacagagata ttagggaata
    22681 taaatcagaa aatggtattc caaatgatta taatgctaag gaaagttttt attacttggt
    22741 ggacaaactt atatcagctg gaatattatc tcaagaagat aaaagattgg atgcgacaag
    22801 attacaggca aaactaaatg aaaatggata ttatccacct attttagtta gtaaatggat
    22861 gttcacagca gaaagagata agagagattg gttggctagt tataagataa aagaaactaa
    22921 actgagtgcc aaagaagcgt ttgaaaaagt aagaaatagt gatgcattag aaattgataa
    22981 aagtttgagt gatgaagatg caagaaaaat aatggtcgta agggatttga taaaatcaaa
    23041 aggatattct caatataatc cagtaactat tgctaaggat gtgggagaaa ctacgattgc
    23101 tcaaattgaa gaaagtgcta tggaccttgt tggtgtttca atagcagtag aaccagtaag
    23161 atattatcct aatgggtcat tagcttctca tatgttagga tatgtaggaa aaatgccatc
    23221 cactcaaata gaatcatacc ttcaaaaagg ttatgaaact ggagatatgg taggtcttgc
    23281 aggagtagaa aagagtaatg aaagtagatt aagaggaact gatggttata aaatggttaa
    23341 agttgatgca ttgggtagaa tcagtaaaga aattgaatct aaaaaaccta aatcaggaga
    23401 tactgtgtat cttacactag ataaagattt acaagaagtt tcagataatg cattaaaaca
    23461 gataatcgaa gttgcttcta aaggaggtac ttttaaaagt aaatttgggg acaaaccaat
    23521 tagtgcatat gcaggaaaag cacagtcagc agctcttata gctattgatg ttaaaaatgg
    23581 agaagtatta gcttcatcaa gttatcctaa ttatgaccca aataaatttg caaaaggtat
    23641 ttctacagag gattataaag cacttcaacc aaaaaatcca aatgacttac tagcaggaag
    23701 tccactttta aacttggtta cacaagggga atttcagcca ggttcttcat ttaagatggt
    23761 aacttcaatg gcagcgttag aaaatggtct ggatccaaac tttactataa atgaccctgg
    23821 ggttataatg ttaggtaaaa aatcttttgg tgactatgta tggaaccatg gtagaggaaa
    23881 ccatggtatg acaaacttat ataaagcaat acaagagtct tgtaatatat atatggcaac
    23941 aattggtaca ggtaaaacat ggccagatgg aaaaagcatt ggtatagata tgaatgcaaa
    24001 taaaatactt gagtatgcaa aattatttgg gcttgaccaa aatacagggc ttcaagatga
    24061 agtagaagaa agagcaggta aagttcctag cacagaagat aaattgaaat caacacaagc
    24121 tcttctaaag tcaaatttag aaagagaaat ggcaaatgat tttgttgata taactagaga
    24181 aaaaaatcct aaagaatatg agaaaagaat caatgagatt gtaagctggg cagcagaaaa
    24241 gaaaactcct ggtagagttg aaacaatgaa taggctaaaa aaattggatg ttaaagaaga
    24301 tagaattgaa gaagtagcag atttagcagt atttagttat tttaattttg caaaatggag
    24361 tactgcagat acattcaact tagctatagg acaaggtgaa aatgcttata caccagctca
    24421 aatatcaaga tatgtagctg ctatagcaaa tggaggtaat ttagtagaat tatctgtagt
    24481 tgatagagct gtttcaagtg attattcatc agttaaaatt aatgaccaga agaaagtcga
    24541 aaaaatacct tttaaaaatc cagataactt aaaagaatta acaaaaggta tgaaattggt
    24601 tgcaagacaa ggtacagcga agagtgcttt tgctgatttt ccaatagatg tagcagcaaa
    24661 aactggtaca gctgagaaaa gtgggaaaat acctacagat aacgagtatg aatatttaaa
    24721 aagtcatatg tcatcttata atgtcaacct aaatgatgcg attaaactag ctgataagat
    24781 gaaagcagaa aaagaaaaag agctttcatt agctaaggaa aaggaaataa aaaagaaatt
    24841 agaaaataaa gatttgaaag acgaagaaag aaagaaatta gaggaagaat tagaagatgg
    24901 agtaaaagtt agattagagg acactgataa ggtgaattct tcatatctaa gaaaagctat
    24961 aaaagaatta aatccaaaaa taacagatga ccaaatagat agatttaaac aagattatgg
    25021 ttcttttacg tggactgttg cttttgctcc agcagatgac ccagaaatag ctgttgtatg
    25081 tgtaattcct caaggtgatt caagtgtatt ctcactttta ccaactagag aagttattgg
    25141 aacttatatg ggattagagc caactaatag taaaaatgac aacaagacga atgatgttaa
    25201 caattcttct gacgaaaata taaatttcga gtcacaaatt aatagatagc tcaatctatg
    25261 tataaacttt agctttatat agaaaaaata aaagggttta aagcgtattt gaagaatata
    25321 aatgatatat cactctggag gttgttatgt ctttaagaga aatttgtagt caagaactag
    25381 tagagttcaa gggtaataaa agagggataa tagtaaatat aaaaagagaa gctccatttg
    25441 aggaaataca ggaaaaaata ataaacaaat tggaagcata tgttggcttt tttaatgggg
    25501 ctaaaattag taaaattaat agtgattgtt taactgacat ggagattcta gaacttaaag
    25561 aaggaattac ttctagattt gatgtggaat ttgttgaaga tcaaaaaatt gaagaaaata
    25621 gtaattttcc aacaaaatat gtcaataccc taagatctgg tgaaaatatt gaatttgaag
    25681 gtgatgtagt tatattgaat gatatgaaac ctggttcaaa agtcctttca aaatcaaata
    25741 cagttgttat gggagatata aatgctggtg ctaaggttgt agcaggaggt aatgtttttg
    25801 taatgggtaa aatagaaggt tttgtacatg caggtgcaga aggcaatgaa tttgcatatg
    25861 ttgttgctgg aaatcttaat cctaaaatat tacaaatagc agataatatt gcagaagctc
    25921 cagatgatga agaaaattat gaaagtgaat cagaaattag cccagaaata gcctttgtaa
    25981 gtaatggaag aattgtaatc gaaagctatt tgtcaaagtt agacaaatag aaagaaaaat
    26041 agggggtagt attatgagcg aagttatagt tataacatct ggtaaaggtg gggttggaaa
    26101 aactactacc gcagcaaact taggaactgc gctaagccta gaaaataaaa aaacagtagt
    26161 tgtggatgca gatattggac ttagaaactt agatgtagta atgggtcttg aaaatcgaat
    26221 agtttatgac atagtagatg tagttgaagg aacttgtagg cttaagcaag ctctaataaa
    26281 agacaaaaga tttgataatc tatatttatt accagcagca caaactagag ataaaaatgc
    26341 tgtctcagtt gagcagatga ttgacctgtg tgagaaacta aaagaatctt tcgagtatat
    26401 aataatagat tgtccagcag gtattgagca aggctttaaa aatgcagtag ctggggcaga
    26461 tagagctatt gtagttacta atccagaaat atcagcagta agagatgctg atagaataat
    26521 aggtctatta gaagcaaatg aaataaaaga gataagatta gttataaata gaattagaaa
    26581 tgatatggtt aagcgtggag acatgatgga taaacaagat ataatagaaa tattagcaat
    26641 agatttgtta ggtcttgttc ctgatgatga aagcataatt atatcaacaa ataaaggaga
    26701 gccagctata cttgattcta agtcacttgc tggtcaagca tacaaaaata tcgcaaaaag
    26761 gatactaaat gaagaagttc ctctacttga tcttgaagtt gaagatggat tctttggtag
    26821 acttaaaaaa atgtttagca tggctaagta gcaaaggagg atacatctgt gttagattta
    26881 tttagagttt tttctaacga ggctaaaact agtaaatctg ttgctaaaga gaggttaaag
    26941 ctagttttag ttcatgatag agtagattgt tcaccacaac ttttagagat gataaaaaca
    27001 gacattttaa aagtaatagc taattatgca gaaatagaag atgatggtct tgaaataaaa
    27061 atgtctaaat gcagaggtga gcatgatgac aaacctgtat ctgcattagt tgctaatata
    27121 ccactaaaga atataaaaga tagatgtatg taattagtgc ttaaaagggg gatatcctta
    27181 ttggtatatg ccccttttat ttaattatat gtttaaagat agaaagacgg tgattctttg
    27241 aaaaaaatca atattaatta taagtcgaca attaagttga taaaacagtt agactggaaa
    27301 cttatagtta cagtattagc tatctttatt tttgggcttg ttatacttag tagtgcaaca
    27361 catgctaatt caacaggttc atataatcaa ttgattaaac aaggattagc ttttgtactt
    27421 gggataggaa tgattatagt catattattt tttgactata accttctagg caggtattat
    27481 aaagcgcttt atataataag tcttattttg ttagccatag ttttattacc tggaattggg
    27541 acagtaaaag gtggagctag gtcgtggatt aacttaggac cacttgactt gcagacatct
    27601 gaaatagtca aattaacatt tgtactaagt tatgcaaaga tacttgaatc caaaaaggat
    27661 aaattaaata cgttaaagga agttatgcct gttgtagttt attctcttcc atttatcgga
    27721 ttattgattg cacaaccaga cttgggaaca ggaattgtat tttgttgtat gatatttgca
    27781 atgctgttca cagcaggtct tagctcaaag ttgataaaaa gaggaataat catactatta
    27841 gtgtcaatgc cactgatgta tttaatgatg gcagaccatc aaaaggtaag aatagaagct
    27901 tttctaaatc cagaagatgt aactctaaaa ggaaactatc aagttatgca atctctgatt
    27961 gcaattggtt ctggaggcgt aactggtaaa ggactttaca atggaagtca aaatcaagaa
    28021 gattttttac ctgtacaaga tagtgatttt atatttgctg ttgtaggtga agaacttggt
    28081 gtaataggaa tggcagtgtt gataatctta tttatgatat tcctattaag gcttttagca
    28141 attgcaagag atgcaaaaga tttttatgga accctcatag tggttggtgt tatgggaatg
    28201 tttggttacc agataatcca aaatataggt atgacggttg cgttaatacc agtaactgga
    28261 gtaacattac cttttgtaag ttatggagga agttcgttat tgacatcttt agcaaactta
    28321 ggattagtac taaatgtgtg tatgagaaga aagaaaataa atttctaaat ttaaatacat
    28381 ctaaacataa attgaggaga ttagatatat gaatatagca ttagtagcac atgaccaaat
    28441 gaaaaacact atggtcggat tttgtatagg ttatgaatca attttaaaaa agtatggact
    28501 atatgcaact ggaactacag gaaaaagaat aatggatgaa actgaattaa atataaatag
    28561 attagcatct ggtcctttag gtggagacca acaaataggt tctttaatag taacacaaga
    28621 aatagattta gttatttttt taagagaccc attgacatct caggctcatg aaacagatat
    28681 acaagcgtta ataagacttt gtgatgttta tcatgttcca atagctacaa atctagcttc
    28741 agcagaaata tttataaaag ctcttgatag aggagaacta tcatggagag aagttagaaa
    28801 aagcaaaagt caacgtattt aaaaaatatt aatattacag tctaaagaaa cagatatata
    28861 agtgtttaat gccctagtct ttctgttgga ataaatctag tacctgtaga aatcttatga
    28921 aagatttaaa tgatgaagag ctaaatttga gattaaataa ttaggaaata gattatagat
    28981 agagtagtag aatttaaact attactctat tttaataata tgaaatagaa tcaagtctat
    29041 tattatatat atttaaactt ataggtaata ctatttcaac caagtaaatg ttgttatgac
    29101 aacattaata aaacaaaaat ttatttttaa aatttgataa atagctgtat attagtattt
    29161 atagattaaa tattaatata cagctattta ttatttttaa gtctattgta gaatataaac
    29221 cttgcaatat agagtactat gtattattat agtaaatata agtactatac attatagagt
    29281 agtaggtgaa aaggcggtgg tacataatgc agttaaataa agaagtgtta aaaggttata
    29341 tcgatatatt aattgtttct atattagaaa agaaagattg ttatggatat gaaattgcaa
    29401 agcaagtaag agaaagaagt gagtttgaat taaaagaagg aacaatgtat cttgcactta
    29461 aaagaatgga atctaaaaat ttaattaaat cttattatag caatgaacaa agttcaggtg
    29521 gaagaagaaa atattataat ttaactaatg aaggtaaaga ttttttagaa ataaagaaac
    29581 aagaatggag atttattaaa aaggttatga atcaattttt gggagaggtg gattaattta
    29641 tgaaactaat agatgaatat ttagataaac tatataaaaa atgtgacaat aaaagtacta
    29701 tagaattaaa acaggaaatg agatgccatc ttatagaatc tgcaaatgaa tttaaattag
    29761 aaggattaga tgaagaagaa gcatgtaaaa aagcaattga aagatttgat gatggtgatg
    29821 aaatgcagta tgaattatgt aatataataa aagaactatc tttatcattg gataggcata
    29881 aatcaattgt aatggggttt aaaaaagtat taggttacat aagtataatt gcatttttaa
    29941 tttctggatt tatgtggtat tacaataata gtttacaaca taatatgtat aatttaggaa
    30001 aagaacttga tggagaaata aagcaattgg cagaaagaca tgatatgaca aagattggtg
    30061 aatataagtt agaacttgaa aaaatacttg ataaagataa gtatagtaaa gtcaaagctc
    30121 ttagactata tgtaatagat atgaaagatg gaaacactaa tttaagttct tctggattga
    30181 atgctaatat ggtatatgag agggaagcag attataataa cataagtaat tttatacagc
    30241 atttaggata taatggaaaa gattttttag acaagaatgg gaatatagta aatccagata
    30301 ttttcttaga atatttcttt tattttgaat cggagatgtt aattccagta gcatttgcat
    30361 ttggattatt atgtataata gcgtatttta tattaagatt taaaatttct ttaataaaaa
    30421 ataataatta aataattcac caaataatct taatttgtag agtaaataag atgtttcaaa
    30481 ataaaatttg aatcatctta ttttttattg tttgttatac tcttttcttt tatatttctc
    30541 catagtggat tgtatctata cataatcaca tattttgata taattatact ttccaaaaaa
    30601 tgtattgaat atacaaatta taatctgtat aatgttgtta ggggagaatg tatgaagtgt
    30661 atacaatttt aattttaaat gtattattga caattctcct cactctcgcc attaccaata
    30721 attggaaaat tgacatacct gttacagacc aacatttttg aatatattaa ttattatcat
    30781 tttattgtga tgatatcaaa aatgcaggtt tgtgagttgg attgtatacg aactatatag
    30841 gaaatataga acagattttc caagaaatct aaatattcta tgaccacgtg aattgctatg
    30901 catacatgtt gataatccat attttaaatc taaaatgaac ttaattaatg gtagggaatt
    30961 gaaagtaaaa tatattataa aggagaatgc taatatgaaa aaaatcagga aattagctac
    31021 taaactattg attgcaatga tggttttaac ctcatgtatg agtgtaacag tttatggtat
    31081 gacagctaaa gatgttactg caaagacacc gaaaagttat gtagctggaa ccaacagtat
    31141 atatggtcca aagttatcac aagctcaact taattcggta gcacaagcaa cagcagactt
    31201 tatgaacaag aaaattacaa agaacatgac cacagatgct aagattaggg ttgcttataa
    31261 tcacattaaa aacaacacaa catatgttaa ctggaacgca gcagagggag ccaatactgc
    31321 ttatacattg gttactaaaa aaggtgcttg ttctggaatg tgtaggagct tgaaagcttt
    31381 atgtgatgct atgggaattg aatgttatta tgtacatagt acaacaaatg accatcagtg
    31441 gaatcttatt cgttttgagg atggacgttt gtatcatgta gatattgatg ccaacaaatc
    31501 agcaggagca gacatcatat acaagagtct tactcaccca ttaccatttg acaagaaagc
    31561 atatccagct gttggcgaag tagtaaagac acagaatcca acgccaactg ttaagaaacc
    31621 tacgccagct aagccttctg cgccagttgg acctgggaat cacactatga tagcagatca
    31681 acaaggggca atacttgatg aagtttatgg aaataatcta cgtccaaaaa ttgaaatgta
    31741 tgagggagtt ccgtgtagtt taatcaactc atcaataaat acaattaagt ttacaagcaa
    31801 aaattcaaat ttagacccat caaagcatac agttactgta atagggttta tgaatacacc
    31861 atatggtatg gttgcattag ctccagatgg tgtacctgat gagtatcctt ttgaatatga
    31921 taagccattt tctttagatt ttactggtaa gaacctttct gcagcagaat tatcaaaaaa
    31981 gtgtaaagaa aataatgtaa ttattaaagt ggaaattggt gaaacggcat ctaattctgg
    32041 aagagggatg gtagcatacg ctagatatgt gaattaatac aaatatacta gtgtacaaat
    32101 agaacatatt ataaataatg caccctcgca caaagggtgc atttatcatt atatcagtca
    32161 ttttagaaaa taaaaaagga gttgaactta aaattagatt agaaaaatta attttgagac
    32221 aactccattt taatttattt ttaaatataa aatcatttat tttaaataat attatgtttg
    32281 attaatggct atttaactaa ttttgcaaat tctgcaccaa atgccataca ctcttttaat
    32341 gtctcttcac tagggaagaa ttttttctta agagattcta caggcatttt gaatgacata
    32401 gatttaagta aagtctcagc catagttata ccttcaccac tccaaccaaa tgaaccaaat
    32461 actcctgcta tcttgccttg gttagccatt gggtctataa cagagaataa atcccacata
    32521 ggtttaacca tagttttgtt tatagttgga gaacctagta atattacttt tgacactact
    32581 aatgtatcgt gcatttctgt taatgtcata tcttctaaat catataattc agcctttgca
    32641 ccttctttat caagtccttc ttttatcttt ttagccatct ctaaagtatt actataagct
    32701 gataagtaga atatagaaac ttgattttga ttagttgtat taacagcttc tgtagaccac
    32761 tcaacatatc ttttaacagc agccattggg tctttagtaa gcattggacc atgagaagtc
    32821 aatatagtat caaattctat gtttagtcct accaccttat ctacagcact taaaacatgt
    32881 ttagcaaatg gcttaactat acaatcatag tagtgtttag ctgattttag ataatcttca
    32941 ctattcacta cctctgcatc aacacttgca aagtgacaac caaatgcatc acaagttaaa
    33001 agagtcttgt cctcctcaat gtaagtaaac attgtatcaa cccaatgtaa gaatggagca
    33061 gttataaatc taagatttct tttaccgata tttaaaattt caccatcttt tattacatga
    33121 cagttaaaag gtctatttat ttgaccatct aagtatagtt tagcagcttt agtacaataa
    33181 acttctatat taggatttat atctaataag tattttaaac tacctgcatg gtctggttct
    33241 gtgtgatgaa ttacaacata gtcaatttta gctatatcag taacctctga taaattactt
    33301 aaaaactcat ctttaaaatt tgcctttaca gtgtcaaata aaaccgtttt ttcgtccttg
    33361 attaaataag agttgtaagt tgtcccaaac tcagtttcca ttattatgtc gaatactttt
    33421 aaaccttcat ctactactcc agtaaagtag atatcttttt ttacttcaaa aactttactc
    33481 atttttaacc ctcctaaaat ttatcattat ataagtaaca taccccattt tgaggtgaat
    33541 gaaacaaaag atgtcatgat aagtcatgta aaacaatagt
//
LOCUS       DAEMGG010000031        32870 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEMGG010000031 DAEMGG010000000
VERSION     DAEMGG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.031"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(90..1097)
                     /locus_tag="KPK24_001450"
     CDS             complement(90..1097)
                     /locus_tag="KPK24_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG2299131.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            1325..2209
                     /locus_tag="KPK24_001451"
     CDS             1325..2209
                     /locus_tag="KPK24_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2299132.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            2411..3961
                     /locus_tag="KPK24_001452"
     CDS             2411..3961
                     /locus_tag="KPK24_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2299133.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETEDLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            3990..4637
                     /locus_tag="KPK24_001453"
     CDS             3990..4637
                     /locus_tag="KPK24_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG2299134.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSILLFVMYKLCKSLSIKIFQC"
     gene            complement(4779..5039)
                     /locus_tag="KPK24_001454"
     CDS             complement(4779..5039)
                     /locus_tag="KPK24_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG2299135.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMSKFQDLVKITSKK"
     gene            5256..6539
                     /gene="hflX"
                     /locus_tag="KPK24_001455"
     CDS             5256..6539
                     /gene="hflX"
                     /locus_tag="KPK24_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG2299136.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            6547..7224
                     /locus_tag="KPK24_001456"
     CDS             6547..7224
                     /locus_tag="KPK24_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG2299137.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            7310..7753
                     /locus_tag="KPK24_001457"
     CDS             7310..7753
                     /locus_tag="KPK24_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2299138.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            8058..8702
                     /locus_tag="KPK24_001458"
     CDS             8058..8702
                     /locus_tag="KPK24_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG2299139.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(8788..9165)
                     /locus_tag="KPK24_001459"
     CDS             complement(8788..9165)
                     /locus_tag="KPK24_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG2299140.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(9165..9551)
                     /locus_tag="KPK24_001460"
     CDS             complement(9165..9551)
                     /locus_tag="KPK24_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG2299141.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            9730..10485
                     /gene="nadE"
                     /locus_tag="KPK24_001461"
     CDS             9730..10485
                     /gene="nadE"
                     /locus_tag="KPK24_001461"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG2299142.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            10568..11287
                     /locus_tag="KPK24_001462"
     CDS             10568..11287
                     /locus_tag="KPK24_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2299143.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(11454..12458)
                     /locus_tag="KPK24_001463"
     CDS             complement(11454..12458)
                     /locus_tag="KPK24_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG2299144.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQEFILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            12724..13620
                     /locus_tag="KPK24_001464"
     CDS             12724..13620
                     /locus_tag="KPK24_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBG2299145.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREMNLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            13758..15020
                     /locus_tag="KPK24_001465"
     CDS             13758..15020
                     /locus_tag="KPK24_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299146.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVDYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            15096..16289
                     /locus_tag="KPK24_001466"
     CDS             15096..16289
                     /locus_tag="KPK24_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG2299147.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            16918..17712
                     /locus_tag="KPK24_001467"
     CDS             16918..17712
                     /locus_tag="KPK24_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG2299148.1"
                     /translation="MESVMENLNNLKVELKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            17807..19147
                     /locus_tag="KPK24_001468"
     CDS             17807..19147
                     /locus_tag="KPK24_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2299149.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            19284..20516
                     /locus_tag="KPK24_001469"
     CDS             19284..20516
                     /locus_tag="KPK24_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG2299150.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            20556..21698
                     /locus_tag="KPK24_001470"
     CDS             20556..21698
                     /locus_tag="KPK24_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG2299151.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            21709..22503
                     /locus_tag="KPK24_001471"
     CDS             21709..22503
                     /locus_tag="KPK24_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2299152.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            22514..23536
                     /locus_tag="KPK24_001472"
     CDS             22514..23536
                     /locus_tag="KPK24_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG2299153.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            23635..25011
                     /locus_tag="KPK24_001473"
     CDS             23635..25011
                     /locus_tag="KPK24_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG2299154.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(25343..25687)
                     /locus_tag="KPK24_001474"
     CDS             complement(25343..25687)
                     /locus_tag="KPK24_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299155.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            26175..27185
                     /locus_tag="KPK24_001475"
     CDS             26175..27185
                     /locus_tag="KPK24_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG2299156.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            27321..27809
                     /locus_tag="KPK24_001476"
     CDS             27321..27809
                     /locus_tag="KPK24_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2299157.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            27985..29913
                     /locus_tag="KPK24_001477"
     CDS             27985..29913
                     /locus_tag="KPK24_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2299158.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            30090..30500
                     /locus_tag="KPK24_001478"
     CDS             30090..30500
                     /locus_tag="KPK24_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG2299159.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            30522..30824
                     /locus_tag="KPK24_001479"
     CDS             30522..30824
                     /locus_tag="KPK24_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG2299160.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(31041..31733)
                     /locus_tag="KPK24_001480"
     CDS             complement(31041..31733)
                     /locus_tag="KPK24_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG2299161.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(31759..>32870)
                     /locus_tag="KPK24_001481"
     CDS             complement(31759..>32870)
                     /locus_tag="KPK24_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2299162.1"
                     /translation="PFQNPYLSACNVSTNMIGIIIGAYGISQCLLRLPVGVFADSINK
                     HKIFILSGIFFAGLASLIRVFSPGGNGFLIANILSGISSSTWISYMVHYTNFFSTEHQ
                     QKATSTIILVNNLGMLIGFVMSTLFYEKLGMTALCIMSVLSSVIGTILALNIKNHNSK
                     KIPKKISIKKYLSICKDKRLVFFSFMALIQQGIQMSTTMSFTTQILKNLGSSSTLIGL
                     SSIFYMVCAVASSGFASTKFCTKYGPKFWIPLVFILVSAYCFLVPTIKNINIIFVLQV
                     LPGISTGILLSYLTSESMKNVPKHQKSTAMGFFQAIYAIGMSVFPMIVGKISEYTSIQ
                     YGYFILAIIAILASILSYTYYKKVDLTLNYDIIQS"
BASE COUNT        12470 a         3772 c         6031 g        10597 t
ORIGIN      
        1 tttggtctgt aggttaaata aaagtacctt tagaccaaat atattattaa taacttaata
       61 acatcttaaa acttatttat ttatattatt tacaacatcc atttaataaa tacaccataa
      121 cctacagctg ggtcgttttc aattgcatgt gacactgctc cgattatttt attatcttga
      181 actattggag taccactcat accttgtaca ataccaccag tttgagctaa aagcttttta
      241 tctgtaattt taattttaaa acttttactt tctggatgtt tttggttttc tatatctata
      301 atttcaatat tatatttttt acacttatta aagttatctt gtaaaattat ttgtgctttt
      361 cctaatttaa cttcatctaa agatgctact ttatattttt ttaacttgga aatatcaaag
      421 ttattaattt gacctcttat tccaaaatca gtattttctg taaagctacc aataatattt
      481 tctcgttttg catttataca tccaacacta cctttataag atttttctat attaaggtgc
      541 ttagttgtag aaattgaacc tgttttaata ggtatttttc ttgcattacc aacacttatt
      601 ggatgaccta cagctccaaa ctctgatgta tctggattga tatacgttaa agtagcaaat
      661 ccagaaagta aattgttaaa actaattttt tctaatatgt gtttggtagt tttcattgtt
      721 ataagttgtc cacctctatt taataaaata gatacttgtt tttcttctgg taaagcattt
      781 aaggtattgg caaagtcact atatccatca acttcagcat tatttacctt tactatagca
      841 tcacctaatc taaatggaga tgcttctcca ggatttctaa ctatgatatt ttttaattct
      901 gcgtcaattt gaagtacgtt tccaattggt attaaatatt caggttcatt tttttgtaaa
      961 ttttgtgcat aaattaaatt atttgagaaa aaatataata aaaataaaaa tgttaaaaca
     1021 attgtaaatt ttgacttagc taagttggtt tttttactta aagtattatt tttaaaatga
     1081 aaatttttaa gttgcataaa acacctcttt cttattaaat tattttaata atagaattga
     1141 agttttcttg ctgcttctat attatgttgt gcaagaatca tgttttgatg cttggaaaac
     1201 tagctcaata acaccttagt tttcataaaa ataaataaaa taaatagtgt atatgataaa
     1261 ataaatacat attcatataa ataaaaataa acaataactg tctagcataa gaaaggagca
     1321 aaaaatgaaa aaatatagat tgttagttac ttgtataatt gccatgggaa tcataattat
     1381 aggaacatca aatccagtta gcaattttta caagactaca aatttgatta gttcaggaaa
     1441 tatcacaaat gaaaaagcag ataaagatga aaattcaaac aaaaataata aagataaaaa
     1501 tgaagacaaa tctaattcta aaagtaataa agataagtca tcagaggaca aatcgtcaga
     1561 taataaaaat gaaattcaaa aaaataaaaa acttttaatt tgtatagacc cagggcatca
     1621 aggaaaagga gatagcaatt tagaaccagt agctccaggt tcttcctcta aaaaagcgag
     1681 agtatcttca ggaacagagg gtattgctac aaaaaagcca gaatatgttt taaacttaga
     1741 agcatctctt gttttaaaga gtatattaga atctaaaggt tataatgtaa taatgactag
     1801 agagacacat gatgtaaata taagtaattc agaaagagca atacttgcta atgacaaaaa
     1861 agcagatatg gttgtaagaa tacatgcaga tagtttaaat aattcttcaa aaactggagc
     1921 atctatattg attcctgaaa aagatggaaa atatacagct cctatatatg aagaaagtaa
     1981 taaatgtgct gaatttatca aacagaatat ggaacaatca ggtatacaaa tcaatggaat
     2041 cgttcaaaga ggtgatttga ctggattcaa ctggtcaaaa gtaccagctg tattagtaga
     2101 aatgggattt atgagtaact ataatgaaga ccaaatgatg tcaaatccag actaccaaag
     2161 aaaaatgatg cagtgtatag ctgatggatt ggatgcgtat tttaaataag atatgaatag
     2221 agaaaaagtt aggttggtat tattttccta atttttatct ctatttttta ttgtacataa
     2281 cacaaagaag aagtttattt taggttaaat tgtcgtaaat ataactaaaa atattgggaa
     2341 tacttgattt aagtttaaaa agaatatatt atagtataga atgtgtaaaa tttttttgag
     2401 aggtgttgaa atgctagtag tagaaaatgt gagtcatgga tttggtgcga gaacaatatt
     2461 agaaaatgta tcctttagac ttagaaaagg tgaacatatt gcactagttg gtgctaatgg
     2521 agaaggaaaa tcgagttttt taaatataat aacaaagaag cttatgccag atgctggaaa
     2581 tataaaatgg tcttcaagag ctacagtagg ttacttagac caacatacgg tattaagcaa
     2641 gggaaaaact ataagagaag tgcttagaga ggcttttaag catatgtttg acttagagca
     2701 agaaatgatt gctatgtatg acaaaatggg agaagctagt gatgatgaaa tgagtaagct
     2761 tcttgaagaa acagcagaga tacaaactat acttgaaaat agtggttttt atatgataga
     2821 tgcaaagata caagaagttg caaatggtct tggtcttgga gaaattggtc ttgataaaga
     2881 tgtaactgac ttaagtggag ggcaaagaac aaaagtactt cttactaaac ttctacttga
     2941 aaatccaact attcttatat tggatgagcc tacaaactat ttagacgaag agcatataac
     3001 atggcttact aagtaccttc aagagtatga gaatagtttt gtcttagttt ctcatgatat
     3061 tgaatttata aataatacat gtaatgtaat atatcatatg gaaaatggag agttaaatag
     3121 atataaagga aattatgatg aatttgtaag acttaatgat ataaagaaac gtcaagaaga
     3181 acaagcttat gataaacaag ttgaagaaag aaaaagacta gaggattttg tagctagaaa
     3241 taaagctaga gtagcaacta gaggaatggc taacagtaga caaaagcaac ttgataagat
     3301 ggaaatatta gaaagaccaa aagaaaaaat aaaacctact tttgctttta aagatgctag
     3361 agcagctagt aaaataattt ttgaaacaga agatttagtt ttagggtatg atgaagcatt
     3421 gacaaaacca cttaatttcc atttagaaag aggaaaaaag attgccttaa aaggaatgaa
     3481 tggtattgga aaatctactc tattaaaaac tttacttgga ataataaaac catttgaagg
     3541 aaatgttaaa ttaggtgact atttggaagt tggatatttt gaacaagaga gttcaagaga
     3601 aaatagtaat acacctatgg atgaagtttg gagtgaattt ccagggctta caaactttga
     3661 agttagacag gctcttgcaa aatgtggact tacaaatgaa catataacga gtcaaatgag
     3721 agtattaagt ggtggagaag ctgctaaggt tagactttgc aaagtaatgt taaaaaatat
     3781 aaatttctta gtattagatg agcccacaaa tcatttagat gttgaggcaa aagatgaatt
     3841 gaagaaggct atcaaagagt ttaagggaac agttctttta gtatgtcatg agccagaatt
     3901 ttattcagaa attgttgatg atgtatggaa tatagaagat tttacaacta aaatcgtgta
     3961 gtgatttaac ttattgctgg ggggatttca tgaaaaagtt tctaaatgta cttttttcag
     4021 tttgtattag tattgttata atagtaggtg ttattaattt tactgttgga tttaaacaac
     4081 tttattattt tgacattgat tacttaaata tatcagagtt atctggttta tctaaagatg
     4141 atataaaatt gaactacgat tatctaattg attataactt aaataagaat gtgtctgagt
     4201 tcaaattacc aacattaaaa tcttctccac aagggaagat acattttgaa gaagtcagga
     4261 atatatttca aaatataaat aaattagcta aattattatt ggtagtttct ttagtaggaa
     4321 ttatattgag tgtaaaaaat aaaaatataa aaatcttaaa aactacatca ataacactta
     4381 taattatgcc attattatta acagttccaa ttttattaaa ttttgaaaag tcatttatta
     4441 tttttcataa attattattt agaaatgatt attggatatt taatcccgat ttagacccag
     4501 ttataaatat attgccagag gaattttttt ttcactctgg aatgatgatt ttaatattaa
     4561 ttttgttagt aagtatatta ctgtttgtaa tgtataaatt atgtaaatct ttatcaataa
     4621 aaatatttca atgttaaata gagggatatt tttatttcca aaataagata ataaaaaagg
     4681 attagattaa aagttataca ataaaaatag ctgtgcaata atatttagac tatgaaagta
     4741 gttaaaaaat atttatgtac agctatttat aatagaattt actttttact agtgatttta
     4801 actaaatctt gaaatttact cataatgtaa ttgtaattat cttttttgtg aggtaacatg
     4861 caactactgc aatctttgat acctttatct gtatacttaa agtttccacc acagttttct
     4921 cctaaagcat acaaaggaca atagcaaaat agacagttaa actcttctgg tttattagtt
     4981 ttatgacaag ggaaaaattc gcaatctttg tgattaaaaa acttataatt ttcgcccata
     5041 ttttcctcct aattaaataa aatttaaatt ttttatacta tataagattt tggtgataat
     5101 ataattatac taaataattt tatgaatagt cgataatata gtattattaa tcatagtatt
     5161 aatggtataa ttagtcaaca gaaaaataat gtaaaaataa tgtaagtgta ttttgtaatt
     5221 agaatatgtt ttataacaaa aaatgaggtg attaagtgaa agaaattcaa gaaaaagcac
     5281 ttcttgtagg tctgaatttg actacaatag tgaaaaaaaa tgatgatata gacactaatg
     5341 aatctatgga agaattaaaa gaactaacaa aagctgctgg ggcagaagtt gtaggtagct
     5401 taattcaaaa taaacactct gtagatgcag cttattatat aggaaaaggt aaagtagaag
     5461 aaataagagc ctatagtgac tctttagatg ctactttagt aatttttaat gatgaattat
     5521 ctggtgctca aatcagaaat atagagaatg ttgttggaag aaaagtaata gatagaacta
     5581 ctttaatttt agatatattt gctcaaaggg cacttagcaa agaaggtaaa ttacaagttg
     5641 aattagcaca attaaaatat agattgccac gactttatgg aatgggtggt gagatgagta
     5701 gaacaggtgc agggattggt actagaggtc ctggagaaca aaaactagaa atagataaga
     5761 gacatatact taataaagca gcagatataa gacgtgaact taaagaagta aaaaagaata
     5821 gagagacaca gagagttaag cgtttaaagt caaatatacc gattgtagca cttgttgggt
     5881 atacaaatgc aggaaaatct accctgttga acgaattaat aaagacacac aaggattatg
     5941 aacaagaaaa agaagttttt gtaaaagata tgctttttgc aactttagat gtaactttga
     6001 gaaaggctct tttgccgaat aaaaaagaat ttttagtagt agatactgtt ggatttgtaa
     6061 gtaaacttcc ccatgattta gtagaagcgt ttaaggcaac attggaagaa gtacagtatg
     6121 cagatttaat cttacatgta attgatgcaa caaatactag ttatgaattg caaaaaagta
     6181 cgactgaagg tgtattaaaa gaacttggtg taaatgataa aaaacatata cttgtatata
     6241 ataaagtgga caagctagaa ttggatatat atcctaaaag tcaagaagat attgtgtata
     6301 tttctgcaaa acaaggcata aatatggata agttattaaa tatgatagaa attgctttaa
     6361 tggaaaatac ttattcagta agtttgatgt taccttatga gagaggtgat atatttagta
     6421 gaataaaaga taaatataat gtggagaatt ttgagtatgg ggaaagtggt ataacgctag
     6481 atgtagattt agatgaagaa gattttaata tctatagaga gtacatacta gaaaaatagg
     6541 ttgaatatgg atataaactg ggaaaatata gataatcttg aagattattt tataacctat
     6601 cttctttata aagaatctaa aacagtatct caaattagta aaattagaaa tatttcaagt
     6661 actgaagcaa gtgagcaact tatacaagca aagttaaaaa taaaggaaat gcaaaaagat
     6721 gattttgagg catctaagga tattttggat aaatttctag aactagataa aattaagaga
     6781 cttgatttta tggatagctt agatgacgaa aaaatggtat actttaagag aaaggttttc
     6841 aaaagaatcc ttgtagaaaa aaatgctgaa gatttaattg ttttaatttg ggcgacaggt
     6901 gagctaaaag atgataggtt tttgaaattg cttcatcagc tcacaaacca cagacactca
     6961 gatattagaa gaataactta ttcagcaatc agaaagattg aatcaccaag tagtagagaa
     7021 gtactgcaaa aaggtctcta cgacaaaaat gcacagacta ggcagtactg tgcaaaagct
     7081 ctttcaaaat tgggagatga aaacagttta aagattttac agcagttgaa agacaagaat
     7141 aagaattttg aaaaagaata tgtgcttaga gcttatgacg aagccattaa gtcattggaa
     7201 aatgcaaaaa atttgaaaaa gtaatttttt attaaagcaa tttgtttact taatgatgta
     7261 tttctaaagt ttaacaaggt aataaataaa ttaatgggag gggtttcaaa tgatagtaag
     7321 acgttgtgca ggtggagtag tgttttatgc taataaagta cttattgtaa aaaatgatag
     7381 gggtgaatgg acacttccaa aaggaaagat actaggaggg ggacttccat atgagagtgc
     7441 tgttcaaaga gttaaagtag aaactggcat agatgctaaa atgatagatg tggctggcga
     7501 tactatgtat gaatttttct caagaagtag acaacaagaa gtttgtaatg caataatgtg
     7561 gtatgttatg gaagcttgta acactgattg tgtacttgct cctgaatttc aagaaggtgg
     7621 attttataaa gtaaaggatg ctttagaaat gttatcacat cataaagagc aagctttagt
     7681 agaagtatct tataaaaagt ttaaggaatt aaagaaatta gtttcagagg gcgatgatgt
     7741 tccagcaaat taattgtgaa tttagaggat tctgaggaag ggatagcccc ttcctttttt
     7801 aattgaagca gttttaagcg taaattatat agtctcttta aactatgatt tatcaattta
     7861 gagatttagt agtttatatt taaaatcagt aagatatatt taaaataaaa attaaaaagt
     7921 atacaatttt attatataat tgtcttatga tgttgattaa agaattatta aaagtccatt
     7981 tatttttaaa tagaataatt ttataaatat atatttgatg tgtatattag aaaatgatta
     8041 aaataggagg aaatacaatg agcaattaca ggacattaca tgaatttgga acggatgaaa
     8101 taacgattga aaagtctgtt tttattggat atgctaagcc tataaagagt gaagaagaag
     8161 cattagagtt tataaatgaa ataaagaaaa agcataaaga tgcaactcac aatgtgtggg
     8221 cttatactgt aggtaaaaat atgaatatac aaagatatag tgatgatgga gaaccacaag
     8281 gaacagctgg gatacctaca ttagaggtta taaaaaaaga agatttgaga gatgtagcgg
     8341 ttgttgttac taggtatttt ggaggaacaa aattaggtgc aggtggtcta gttagagctt
     8401 atacaaaagg tgctaaactg ggacttgaag caggtaagat aatttataag gtaatgtatc
     8461 aagaagttaa ggttaagata gattatactc aacttggaaa agtgcaaaat gagttaatga
     8521 atttggggta ttttataaaa gatactgtat atgaggataa tgttgaaata gtagtatatt
     8581 caagattaga agatgtagag aaattatcag aaaaaatgat tgatataaca agtggaacag
     8641 gaaaaatagt tttaggagag gaattttatt tgtctgaaca aaatggagaa attttaatat
     8701 aatctaaaag gttattagaa tagttaatac ctcaaatagc caattagtat ttttaagtga
     8761 aaagccatta aattgattta atctaaatca atttaatggc tttttatcgg attttgcaca
     8821 gaatcctttt tttacttcaa ataaaccgac taaaagtaaa aatatcccaa atattttttt
     8881 taatatatct gaagacagat aattagctaa tagtgaacca aatacagctc ctaaaacccc
     8941 aaacacagct ataggtataa ccaatttaaa aattacattt ttatttttta tatgtatagc
     9001 aagtgctata agtgtcatag ggatagatga aagtaaattt acgctttgtg ctattttagt
     9061 gtctatgcca gcaagtaaaa cgagtgcagg tataaggatt gttcctccac ccattcccat
     9121 tcctccaata attcctgcaa aaaatccaat tagagcaaat aacattaaaa caccatcctt
     9181 aatgctgcaa ttatcataat tattccaaaa gaaattctaa gaaattttcc agtaacctta
     9241 tttagtaatt tagctcctat aataccacca agaatacttc caactgcaac tttaattgtt
     9301 aagttaacat cataagtacc tttagataca tataaaacag aacttgctgt cgttaagaat
     9361 attattatag aaagagcagt tgcatgagat ttatgttctt ctaccttaac gatgtcattt
     9421 aatattggaa ctaaaagagt acctcctcca gaaccaaaaa ctccatttat aaatcctgta
     9481 aaaactccaa ttatcgtatt ttttaagtta aaatcaaagt attttttctt taaatcatca
     9541 gtttttttca taaaaatcac cttctgaaat agttttacca aaataggcaa attatattta
     9601 tataaaataa gattaaatat aatctttcat gtttgtttaa ttttgcataa aaaaaaacat
     9661 attaaaacaa catattaata tatggtataa tatattaaaa caaagtagca aatttgagag
     9721 gagataacaa tgtcaaatat aaaaatacaa atagacaaaa ctgtagaatg gcttataaat
     9781 aaggttaatg aggcaaatgc aaaaggatta attgttggtg tatctggagg aattgattcg
     9841 gcagtagttg ctaatttaat taaaaaggca tttccagaaa actctatggg agttataatg
     9901 agcataaaaa gtaatcctca agatagagaa gatgcactta aggttataga gggatgtgat
     9961 atagaatacc ttgatttaga ccttatagaa ccacaaagtg ctatattaga catggtagta
    10021 ggaaatttaa aagataaaca tttatataga gaagaatatt taaagatgac agatgcaaac
    10081 ttaagagcaa gagtaagaat gagtacaatt tatacaattg ctaacaatct aggatatctg
    10141 gttgttggta cagacaatgc tgcagaaata cacacaggat attttactaa atttggtgat
    10201 ggtggagttg atattttacc tattgcaaat ttaactaaag gagaggtgta tgaatgggca
    10261 aaagagcttg gtgttcatga agatttaata aacaaagctc cttcagcagg tctttgggaa
    10321 ggtcaaacag atgaagatga aatgggaact acttacaata tgatagatgc tgtgttagaa
    10381 ggtagattgg atgaagttcc taaaagagac caagagataa ttgaaagact tcatagatta
    10441 agtgaacaca agagaaaaac gcctgcacaa cctcctaagt tctaatttac tatttataag
    10501 atttgtgata aaatttacaa gtgatgaaaa ttagaataat taattgtacg tattaaggag
    10561 tggaaatatg ggacgtatag gtaatataat taatagaaaa ggtaaacaag atgctcaaag
    10621 agcaaagata tttactaaac atgctagagc aatagcagtt gcagctaaag aaggtggagc
    10681 agacccagag tacaatgcag cgttaaaaac agctatagaa aaagctaagg cagataatat
    10741 gccaaatgat aatatagata gagctatcgc taaaggtgct ggagcaggtg ctggagaaga
    10801 ctatgaaact atagtttatg aaggatatgg acctggtgga gttgctgtta tagtagaaac
    10861 tttaactgac aacaaaaaca gaactgctgg taacgtaaga tactattttg acaaaaacgg
    10921 aggaaactta ggaactagtg gatgtgtgtc ttttatgttt gataaaaaag gacaaatatt
    10981 agttggttta ggagatggag tttcagaaga agaattgatg gatgttgcat tagaagcagg
    11041 agctgaagat ttcataacag aagaagatgg ttatgaaata ataactacac cagaagattt
    11101 ttctagtgtt cgtgatgagt taaaagctaa aggatatgag tttatatctg ctgatgtgaa
    11161 gatgatacct caaactacaa ctgtattgac tgaagaatct cacttaaaaa tgatgaataa
    11221 attagttgat atgcttgaag aagatgatga tgttcaagat atatatcata actgggaagt
    11281 agaataaata gtataagcat attgaaattt ataagtattt aatcaatttt aacatgtgat
    11341 aaattttata gaataagaaa cactaaaata gacatacgca cctctaacct atatttgcat
    11401 aataatatgt aagcataggt aaggggtgtt tttatattta taggttaaga aatttaaagt
    11461 aaattttgag ctggactcga ttgtctataa ataattgatg ggtatataac ttgttgctga
    11521 ataccattta tacgcccagt tactgtatca ataatcatcc taagactacg ttctgctgtc
    11581 tcttccattt ttaaatctat aactgtcaaa ggaggaatac ttcttgccat tagtcgtgaa
    11641 ggtccattac ttgctgcaaa tatttgaata tcctctccaa ttacaattcc cgattcatga
    11701 aatgcattca tcataccaag agctaccaca tcatgattac aaaaaattac tttttttagt
    11761 gaattttttg caagcatttt ttgagcaata tcatatccac tatctatttt attagaacaa
    11821 taatatatag aatctgatag atgtattcca tgttctttac atgtttttaa aaaagattta
    11881 ccccttaaag tcatacatgt aaaggaagtt gggttgaata caacagcaat atcattttta
    11941 ccattttgaa tagcatgtaa agcggctata cgaccagttt cctcattatc aatagataca
    12001 cttgaaaaac caggtatttc acgatttaca acaactacag gaattttagg tttatttttt
    12061 actagatatt ctatatcatc agcattgata cctgttaaaa caattccatc ataataatta
    12121 tttgcaaaaa agtattcatc atcacataga tgaccaactt tgtaaggtcg tatgatgata
    12181 tttatgttta aatgttctaa agaaattatg tggtttattg cttgaagtat attggttgat
    12241 gccaactcaa atccctcatc ctgccaataa aaagctattt taatattatt atgtttaata
    12301 ggagtagagc gtttatatcc aagcttatca gctgaattta atataaactc ttgtgtatgt
    12361 agcggaatcc tgcgttcatt tcctttacca cttaagacgt acgagacagt tgctgaagaa
    12421 agaccacagt cttttgcaat atctttgatt gttgccatga taaaatcctc cttaaatagt
    12481 tgcttaaatt tgcttatacc aataatctta acatatattc aactaaagaa caatattcta
    12541 caaggtgcat attttacagg attagaataa ataaatttaa aaaaacttct tacttatgtt
    12601 gacataattt agtttatagg attataatat gcttaaataa gcttattatt aacttgaatt
    12661 aagcaaagtt aagcaggaac atgcaatgct agcttgcaat gtgactgtaa agggggaaaa
    12721 ggtatgagta aggtaaatat atttgaagtt ggtcctagag atggatttca aaacctgaaa
    12781 gagtttttag aaactgataa aaagcttaag attattgatg gtcttattgc ttcagggatt
    12841 aaacaacttg aaattagttc atttgtaagc ccaaaagcaa tacctcagat gaaagatgca
    12901 aaagaagtag caacttattg tgtagaaaaa tatcctgatg tgaaacttta tgcattagtt
    12961 ccaaatttat acggtgctaa agttgcatgg gagtgtggta taagagatat atcctatgtt
    13021 atatctttga gtgaaactca taataaaaaa aatataaatc gtacccatga agagtctatt
    13081 gatgagctta aaaaaataat gaatacttat ccagacatga atatatgtat tggaatggct
    13141 actgtatttg gatgtccttt tgaaggttta ccaaaaattg aagatgccat aagatttgct
    13201 aaacgccttt gggaaaacgg tatcagggag atgaatctag cagatacaat agggattgct
    13261 gaccctaagc aagtaagaga aactattagt gcattagttg aagctttacc agagtgcaga
    13321 tggcaggtac atattcatga tactagaaat atgggaatgg ttaatactct tgtggctatt
    13381 gaatctggaa ttactactgt tcagtctaca cttggaggat taggaggatg cccatttgct
    13441 cctggagcat ctggcaatac tgcaactgaa gatttagtat atatgcttga acgtatgggg
    13501 tatgatacag gagttgattt tgttaaactt cttgaattat caaagtatca gaagagtttt
    13561 gtaaatggag tatatagtgg tcatcatgta aatataaagt ctgatagttg taattgttag
    13621 ttctattgat taatcgatta aaaaaatatg ttgattaaaa aaatatgctg attggtaaaa
    13681 tagatttatt gataaaaaat ataaattata aaatacatta tctaataaaa caaataaaaa
    13741 aattgagggg gattagtatg gatattatag ctttactttt atttttagga gttattatta
    13801 ttgcatttgt acgtaagaat aatgcaggta ttttggctct agctattggt gtcatagctg
    13861 ttagaatttt tggaatggat gataaagaac tttatggagc tataagcaac tcaatgttta
    13921 ctacacttgt tggtataaca ctactctttg caatagtgaa ctcaacagga gcattaaagc
    13981 ttgttgcaga aaaaattgta gctgcttctg gtaaacgtgt ttggcttata cctatagcag
    14041 tttttatagc aggatttgtt gttgctggtg ttggacctgg ggcaattcca gcacttgcaa
    14101 ttatcccagc aatggctgtt cctgtagcca taacggttga ttataatcct gtaatgcttg
    14161 cacttatagg tgaatgtgga ttaatggcag gtcgtatgac tcctattact ccagaaggtc
    14221 aaattataac aaccgttgca gaaggggttg gaattgaaaa tgttatgcct actatcttag
    14281 cctgtcaaac tctttctaca attgtatttt caatagtatt gtttataatt ttcaaaggat
    14341 ataaacttaa aaaacctata aatgttatag atagtaagca aatagaaaaa ttcaatataa
    14401 agcagataat ttctctaata agcatagtag ttatgattgc ttttatcata atttttgata
    14461 tgaatgttgg gcttgcagct tttgcagttt cagctgtact tatttttttc aatattgctg
    14521 atgaaggaga atgtatcaaa tcaattccat ggagcacgat agtaatggtt cttggagtag
    14581 gtgcaatgat gacaatcgta gatgcagcag gaggtattga cttgatgagt aatgcattat
    14641 catcactgat gaatagtaaa acagctactc caattatgag tatttcagca ggacttatgt
    14701 ctatggttag ttctgcttta gcagtagttt atccaactat gatgcctatg tgtgttgata
    14761 tagctaaggc agttggagga gtagacccac tagcacttat tgctgcagtt ggtgttggag
    14821 gttctcttgc tggtgtaagc ccactttcta ctggtggggc attgatttta gcagctatgg
    14881 gttcaagtaa gaaagacttt aataaggaag aacaaaataa ggtttttgta cagcttttca
    14941 ttatgagtgc agttggctta ttggttattg cagtagtatc aatattatca tttaatgcaa
    15001 taacacattt aatgaactaa ttagaataac tgaaaataaa tcattaaatt taacttttaa
    15061 tattaatcaa tataatataa tttggagggg aagttatgaa aaaaggagca ttaagcaata
    15121 taaaggtact tgaccttaca agagttcttg caggtccata ttgtacaatg atgttggcgg
    15181 attttggagc tgatgttata aaaatagaga tgccaagaaa aggtgatgat acaagaggtt
    15241 ttggaccttt tgtaaatgga gagagtttat actatgcaaa tataaataga aataaaaaag
    15301 gtatcactct aaaccttaaa tcagaagaag gaaaaaatat atttttagaa atggtaaaac
    15361 atgctgatgt tgtagtagaa aattacagac caggtgtaat ggaaaagtta ggtctgggtt
    15421 atgatgagtt ggctaaagtt aacaatcaaa taatttatgg agctgtatca ggatttggat
    15481 gttatggacc atattcagaa cgtccaggtt atgatattat tgcacaggca atgggtggtc
    15541 ttatgagtat aacagggcaa gaaggaggag aaccaacgag agttggtaat gctatgggtg
    15601 atgtactagg tggaatgaat ttaactattg gtatactcac agcactcaac gctagaaaaa
    15661 taataggaaa aggacaaaga gtagatgttt ccctcgttga ttctgttgta tcgtctctcg
    15721 aaactggtat acagagatat tttgattcag gacaacctga acttatgggt aatagatatg
    15781 cttcagccta tccttatgac tcttttaagg caagtgatgg aagttttgta ataggctgtg
    15841 gtaatgacaa actttttgaa aagttatgta aaaaagttct ttttagagaa gaccttttaa
    15901 tagatgagag atttaaaaca aatcttaatc gttgtgaaca ttacaaggct cttaaaccag
    15961 aaatagaaaa atggtctgtt aagcatacaa taaaagaagc agttgactta atatcagagg
    16021 ctggagttcc tgctgctcct atctataatc ttgattgtgt tacaacagat gaacatatag
    16081 caaaagcaag agaaatgatt gtagatatta gtcatcctat tataggaaat atgaaagtca
    16141 atggaaatcc agttaagttg atgacaacaa aagctaaaat tgatatgcca gcaccatctc
    16201 taggggaaag taacgaggag atttataatg gttggcttgg ttttgatagt gataaaataa
    16261 aagatttaaa agaaaatgga gttatttgat taaaaaatag ctaaacacac ttgtgacttt
    16321 agtcatggga ggttcagtta gaataatatc aaaaaatatt aaatcaatac tgtaaataaa
    16381 atatagaatt gtcagaagca tttaagatgg gaaaagctac ttagacattg agtctttgtg
    16441 gcttttccta ttttcattaa gaattttata ttatcagaat agatatgtac taaactaaag
    16501 tattaacaat ttaaattaat agaaaataaa ctacctaatt atgttgaata aagactactt
    16561 tagtgaaaat aaacttatag agatgctatt taaattattt taatggaaat atcaaaaatg
    16621 agactgcatt ctcaaaaatg agactgcgtt ttttaagtat tttctcgaaa atgagacaaa
    16681 aatattgttt tatgggtttt attgaagaaa aattcttctg tgaattacaa tttaatcaat
    16741 tttaaagttg aaaattatta aaaatttagt gaaatttatg tatatttcgt ttattttatt
    16801 aaaaataaca attttttact aaaaataaag aacttcaatg gtgattggca tagtacttgc
    16861 tattttatat taacaagaaa tgtagaaaaa ataacaatgt ataatggggg gaatatgatg
    16921 gaatctgtaa tggaaaattt gaacaatctt aaggtggagc ttaaggacaa agtctgtgtt
    16981 attactataa atagacctaa agctttaaat gctcttaata gtgacactct tagggaacta
    17041 tcacaagtta ttgatgtagt atcagaaaat gaggcaatat taggagttat tattactgga
    17101 gaaggtaaag tttttgtagc tggtgctgat atacgccaga tgcaaaacta taaaagtgag
    17161 gaaggcagaa aatatgcagg ttatgctcag ggaatttttg ataaaattga agctcttgaa
    17221 aaaacagtaa tagcagcagt taatggatat gcattaggtg gtggatgtga acttgctatg
    17281 agttgtgata ttagaattgc atcagaaaag gctatatttg gacaacctga agttaattta
    17341 ggagttattc catgttttgg tggaactcaa agattatcaa gacttgttgg aactggaata
    17401 gctaaagagt taatttttac agggagacaa gtaaatgcag aagaagctaa atctattggg
    17461 cttattaata aagtggttcc aagtgattta cttttagaag agtctatgaa gatgatgaac
    17521 caaatagttg aaaaagcgcc aatagcaata agatatgcaa aagttgttat taacaaaggg
    17581 atagatatgg atttaaagaa tgctcttgaa ctagagaaag atattgcagg tttaactttt
    17641 gctacaagag ataagcaaga aggaatgaat gcatttatag aaaagagaaa gccagttttt
    17701 gaaaataagt agctattata actaaaattg gaacaatgta gaatatattc aaaatctaaa
    17761 tttcgtgaaa aaatacatat agtttaattt taaaaggagt taatttatga atacgggagt
    17821 aattataagt ttagtaggga ttatcatagc tctatctctt ctagttgtaa tggttatgaa
    17881 gggaataaat atttttatta tagcaattgt atgttcatca ttagttgcta taacaggaag
    17941 cctaaatgtg tatgatgcac ttaaagtgga ttatatgaca gggtttgttg gatttcttca
    18001 ggcaaatttc tttattttcc taacaggtac attaatggga aaaataatgg agattactgg
    18061 tggagcaaaa tctattgcta agatgattgt aagatggatt gggaaggata aagcactact
    18121 ttctattcca atagcttgtg gaatattagc ctatggtgga gtaagtgttt tcgttgtaag
    18181 ttttgcagtg tttccgatag ctttagaaat ttttaaagaa gctgatttgc cacgaagatt
    18241 tataccagca gctttgactt ttggatgctc aacttttgct atggttgcac caggggcacc
    18301 acaaattcag aatattgttc cagcatcaac attagggaca gatattatgg ctggagcagt
    18361 aaatggtttt atatcatgtg cagtaatgtt tatagttgga agtaccattt tgtacaaaat
    18421 ggtttcaaaa gaaaaagcca atggtggtca ttttatagca catgaatcag atatatttga
    18481 tgaagcagaa gcaactaatt ctaagagaga aaatggtccc aatggtgtaa ttgccttaat
    18541 tccattgata gtgtcaattt taataattaa tgtaaaagtt aatggaaaag cgatagtacc
    18601 aattgaggtg ggagtattta ttggagcttt attagtatat ttattgttaa ataaatatca
    18661 agataatact aaaatagttg gacatgttgg tgatgcatgt aaaactacag tagtagcaat
    18721 atgtaatact tgtgctgtag ttgcatttgg ttcagtagta aaatcagcag ttggttttga
    18781 cttcatagtt aatgctatga ctaatattcc tggtcctcct attgcagggg ctgcagttgg
    18841 aactacagtt attgcaggta tttgtgggtc agcttcaggt ggactaggaa ttgcagctcc
    18901 acttcttgga ccagtatttt tagccaaggg tgtttccgct gcagcattaa ctaggactat
    18961 ggcaatttca tcagcagcat tagattcact tccacacaat ggatatattg ttactgtaac
    19021 aaatggtctt tgtaaagaaa cacataagga tgcctatatg ccaatattct gggtaactgt
    19081 tttaacccca tttattggaa ccatagtagc agtcctactg tttacaatgt tcccgggatt
    19141 gccataagag aaaagataaa aggtataaat tatattttga tattataaaa tcaattttca
    19201 aaatatatac aataaatttc atgttttaat actgtaaata tgtatttaaa ataacaaatt
    19261 gaccatataa ggaggaattg aaagtgttaa ataaaccatt agagggagtt aaggttattg
    19321 acctaacgta ttttgtagca ggtccaggga cttctaaaat attagctgac tggggtgctg
    19381 acgttattaa ggtagagcca agctttggag acccaggaag aaagacaggg gcaacaatga
    19441 caatgccaat agatgattat aataatcctt tttacagcac atataattca aataaaagag
    19501 gtttgtctat aaatcttaag agtgaaactg gaattgaaat tatggataag cttttaagcg
    19561 aagcaaacgt atttgtttca agttatagaa caggagcatt aaaaagacta ggtcttgatt
    19621 atgaatcatt aagtaaaaag catccacacc ttatctgggg tcagataaat ggttttggcg
    19681 attttggacc tgctaaggat aatgcaggat ttgatacagt tgccttttgg gcaagaagtg
    19741 gggctatgct tgatatagca gaaaaagata caagtccaat aaatccacct atagcctttg
    19801 gagatgcaac aacttcttgt agtttatctg gaggaatatg tgcagctttg taccagcaag
    19861 ctaaaactgg aaaaggacaa aaagtaatgg catctttatt tgggcaagca atatggaatg
    19921 catcatcttt attagcatct acacagttta gtgatgagta tccaaaaact agaaaaaatg
    19981 ctatttcacc agtaataaat tcttatcgtt gtaaggatgg aaagtggata ttcttaagta
    20041 ttttagagca tgaaagatat tttgaggcac tttgtagatt atttggaaga gaagacttaa
    20101 taaaagaaga gaaatttgca tcttcagtag aaagtaagaa aaatgctaca gaacttatta
    20161 atattttgga tggtgaattt gcaaagttta ctcaaaatga gatggttgaa aaacttactg
    20221 ctgctgatat agctcatgaa aaaattcaac atgttaatga tgtagtgact gatgaacagg
    20281 ctattgctaa taactatata tatgaacata caagtaaaaa tggaaataag acaatgcttg
    20341 catctactcc agttaaattt ggaaatattg aagttaacat gacttgtgat gctccactta
    20401 taggagaaca ttcagatgag atattgagag aacttggata cagtgattct gatatagaag
    20461 ctctaattga atctggaatt gtaacaatga ataaagaagc atgtgaaata tgttaataaa
    20521 catgtgaagt atgttaataa ataggaggga attttatgga ttttagatta acagaagcac
    20581 aattaatgct tcaaagagta gcaaaagaat ttgcagagaa tgaaatagca ccaatagctg
    20641 ctgaaacaga caaaacaggt atttttccaa gggagctttt tagcaaaatg gctaaaatag
    20701 gatttaatgg aattggaaca cctgtagagt atagtggttc tggtggggca gatatagaaa
    20761 aggtaattgt tgttactgag attgccaaaa aatgtgcagc atctgcagca atattatcaa
    20821 tacacacaat atatgctcaa gctattttga aatttggtac tgaggaacaa aagaaaaaat
    20881 accttccaat gatggctgaa ggtgggtgtg taggtgcttt tgccttaacc gaacctaatg
    20941 ctggttcaga tgctgcgaga gctgcaacaa ctgcaattat tgatgaagaa actgatgaat
    21001 atgttttaaa tggaactaag tgttttattt ctggtggagg tcaagcagaa tcattaataa
    21061 tatttgcact gacagaccca tcaaagggaa taaagggaat gtctgctatc attgtcgaca
    21121 aaggtactcc aggtttttca ataggaaaaa ttgaagaaaa aatgggtatt catggttcag
    21181 aaactgcaga gttaatattt gataactgta gagttccaaa gtctaactta ttaggaaaag
    21241 aaggcaaagg atttaatata gctatgactt gtttagatgg agcaagaatt ggagttggag
    21301 ctcaagctgt aggaattgct gaaggggctt tagaagaaag tatcaaatat tctaaagaaa
    21361 gagtccagtt tggcaagcca atttcagctt tgcaaggtat tcaatggtat atagcagata
    21421 tggcgacaat ggtggaatca gcaaaattat tagtatatta tgctgcagat ttgaaagcta
    21481 gaggagaaaa acacactaaa gaagctgcta tggcaaaata caatgcgtca cgtactgcaa
    21541 gagaggttac aaatttagcc cttcaaatcc atggtggata tggatatatg aaggattatc
    21601 cattagagag aatgtacaga gatgccaaaa taacagaaat ttacgaaggt acttcagaaa
    21661 tacacaaagt tgttatttca agagcagtac taggatagga ggaaaagaat gaatatagtt
    21721 gtttgcttaa agcaagttcc agatactaat gaagttaaaa taaataaaga gacaggtact
    21781 ctaataagag atggtgtacc aagtataatc aatccagatg atagaaatgc tttagaagaa
    21841 gctctaaaaa tgaaagatga actaggagca gtgattaagg ttataagcat gggacctcct
    21901 caagctaaaa gtgctttaaa agaagctttg gcaatgggag ctgatgaagc ttatttaatc
    21961 tcagatagag cttttggtgg ttcagatact tgggctacat ctacaattat tgctgctgct
    22021 atagagaagg ttggaaagta tgatgtaata ttctgcggaa gacaagctat agatggagat
    22081 acagctcagg ttggaccaga ggttgcagaa tttttaggga taccacaagt tacttatgcg
    22141 aaagaagtaa aggttcaaga tgacaaatta ctagtaactc gttatacaga gacaggggat
    22201 tatttaatag aagcaaagat gccagtttta ttaactgcta taaaagagtt aaacaatcca
    22261 agatatccaa gtgtcaaagg aatcttagaa gcttataata atggagatgc aaagattact
    22321 gtattaacac ttgcagattt agatgttgat acaactcaaa ttggattaaa gggttcacct
    22381 acaaacgttt ataagtcttt tgtaccagtt aaagacaagc ataatgaaat aatagaaggt
    22441 ataaataaaa aagaaaaagc tgagaaacta atagaaatac tattcgattt gaaactagta
    22501 taggagtgtt aagatgatga gagcaaaagt taatcaaggt ataaatttaa atgattataa
    22561 tggtgtttgg gtaataggag agcaaaggga aggtaaaatt aatcctgtta cgatagaact
    22621 tattggtgaa ggaagaaagc ttgctgacca attaggaaaa gagcttgcag ttgtgatagc
    22681 tggatatgaa gtagagaaag aagtgaagga attacttcac tatagtgttg ataaagtata
    22741 ttacatcaat gaccctcttc ttaaagattt cactactgat ggatatgcaa tttcaattgc
    22801 aaatttaatt gaaagaaaaa agccagaggt tgtattggtt ggtgctactt ctataggaag
    22861 agatatagcg cctagaattg caggtaaggt tggaacaggt ctaactgctg actgtacaaa
    22921 acttgaaata gattcaacag ataataagtt attacaaaca agaccagcat ttggtggaaa
    22981 tttaatggcc actattgttt gtccaaaaaa tagaccacag atgtcaacag ttcgtccagg
    23041 agttatggca aaagctgtaa gaaacgaatc agagactgga attttagaag ttgttactcc
    23101 agaacttact gaaaaaatga ttagaactag attagtagag atacttccac aagaaaagaa
    23161 atctgtaaat ctaactgatg ctagaataat agtatcagga ggaagaggat taaaaagagc
    23221 agaaggattt gagcttataa aagaattggc tgataaattg ggggcagaga ttggtgcttc
    23281 aagagcagca gtagattcag ggtggataga acattctcat caagtgggac aaacaggaac
    23341 tactgttaga ccagaactat acattgcatg tggaatatct ggagcaatac aacatttagc
    23401 aggtatgagt gattctaaat atatagttgc tataaataaa gatgcaaaag cccctatttt
    23461 tagtatatgt gactatggaa tagtaggaga tttatatgaa attatacctg aaatgataga
    23521 atcattaaat aggtaacttt tagaaaacat tgaatatgta gaatgataag tcttataata
    23581 ttgtattgta agacttatta tttttgctga aggttatagt ggggagatga aacaatgaaa
    23641 gagcagtggt ataaggatat atttgcaaga gtattatcta tgactgatga tggttttatt
    23701 gttgtaaata caagtggagt aataatagat attaatgata aatattgtga ctttttagga
    23761 aaagaaagaa aagatataat aggacaaaat atacaaagta ttataccaaa tacaaaaatg
    23821 ctagatgtta tgaaaaataa gtattgtgaa gaaggagcta tacatcatta ttcaggtgga
    23881 aatacaaaag aaaaaagtgt aatagtaagt aggtcttatg tggaaaatga caatggagaa
    23941 gtagttgcag gagtagctca agttaaattt agattgcaat catttgatgt agctaaaaag
    24001 ttaatgtcag aatatatgga gctccaatat tataaagagc agtttaagga taactgtggt
    24061 tttgacaagc tcataggtga aaatagagac ttcatagaac ttaagaaaac aggtgtgaaa
    24121 gcttctaaaa caaactttcc agtactttta acgggtgaaa caggtacagg aaaagaagtc
    24181 tttgctagag caattcacaa taatagcagt aggtcagata aacctatggt tagtataaac
    24241 tgcgctgcaa taccagaaga attattagag tcagagctat ttggctatga cgaaggagct
    24301 tttacaggag caaaaaaggg tggtaaaaaa ggtaagtttt tagttgcgaa taatggtaca
    24361 atctttttag acgagatagg tgacatgcct ttgaccatgc aagcaaaact tttaagagtt
    24421 cttcaggaaa gtgagataga accagtggga ggtcttaaaa ctataaagat agatgtaaga
    24481 gtaatctctg ccacaagaaa gaatttaagt aaaatggttg aagagggatt atttagagaa
    24541 gacctatact atagattaaa tgtaataaac attcatatga tggaattgaa ggacaggcaa
    24601 gatgatattt tattgctagc aaattatata ttaaacaaat taaatgttga atacaaggaa
    24661 ttgaaggttt taagtgataa ggttaaaaat tgttttatta actatacttg gccgggaaat
    24721 ataagagaac tacaaaatgt aataaaaagt gcctatgctg taagtgatga tatggtcatt
    24781 atgatgtgtg accttccatc taagatggat aatatcagca gagtagctca atgtaatgtg
    24841 gatagcaatt gttcaattca tgaaatggtt gagaattatg aaaagagctt aattattgat
    24901 gtgttaagga agtataattg gaaatgttct aaggcagcag aggttatggg aatacacaag
    24961 agtcttttat ataagaaaat aaaaaagtat gaaattgaat taaataatta agataaattg
    25021 aattaaggca atgctaaaaa agcgccttta ttcaaagcga actgtataaa ctgttttatg
    25081 cagttaaaac gtgtaaaagt agatgtgaag tactaatgtc aatcattagt acttttttat
    25141 tatatttata attactatct atatttcagt atcttttgat atctaagatt attatgttta
    25201 atttaggata taacaaaacc ctcttagtta tggacagttt tttatatttt aactgtctac
    25261 taagagggga tagtttagtt taaatttaga gtaaatttta tctagcattt ataaaaaata
    25321 ttttgaatag cttttttgtt atttagtcat aattttctac ttctttatca agttctttgc
    25381 cacaagaatc acacttatat atagcattgt atccgtaatc atattcatca actattttct
    25441 tttgtctaaa tacaccacca cactcacata ggaaaccaat ttttataagt gtaacgataa
    25501 attgtgattt tataaatttt atatcattac atttatcgtt agtgtaaaat gtataaatat
    25561 aagagttatc gcactcatct cttaattcaa atctttcaat atatgtgtta ttttttaatt
    25621 cctcaaattc ttctttagtt aaatatgatt tttgttgcaa ttcatgtaaa cgcttaattt
    25681 ggtccattat ttatatcctc ccaataattt tagtatttta agcttagata aggaatctaa
    25741 gttttattaa atttatccca aggaattatt taaatcatat ttagttttat taattacaac
    25801 atatataaat tacacgaaaa taaaactaat tgtaaaaata atatcctgtg aaaacctaaa
    25861 ctactaaccc cttactaacc cgaaaactaa aaaaagaaaa tatatccaaa tataagtgat
    25921 atataaataa agatgtttca tataaattta actttaaata ttctgacaat aatatatata
    25981 aatatattat agttaaaata taccacatat tttgggtatt gtatactgga attagagtaa
    26041 ttaatttatt ttttaacata caaatgattt tatgaaaaaa aacaagactc aaaaattttt
    26101 atattctttg atagaataaa attgagagta aatttattga attttataaa atttataagg
    26161 ggtgagtcta agatatgcat gaagataaaa atgatatatt tgaatcaata gatactgagg
    26221 attttgataa taaaacagaa gaagaatgtt tagagctagt aagaaaaaat ggattgaatt
    26281 taatatacat aaaaaatcaa acagaagaga tatgtctaga ggctgttaga caaaattgta
    26341 atgcaataaa atgtgttcag aatcagacag aaaaaatatg tatagaagca gttaaacaag
    26401 attggagaat gttagaattt gtggaagagc caacagaaga aatttgcatg ttggctatta
    26461 accaagatgg aacagcatta aaatatatag aaaatcagac agaggagtta tgcttaagag
    26521 ccgttgaaaa aaatggtgcg gctttggagt atgtaaaaga acaaacagaa gaaatatgta
    26581 tagaagcagt tagaaacagc gaatttggat tggcaagagt gaaaaatcaa acagaaaaaa
    26641 tatgtatgga agcagttaag cattgtagct ataatctaaa atgggtagaa aatcaaacag
    26701 aagaaatatg tatggaagca gtgagacaaa atgggcttga tttgaaattt gtaaaaaatc
    26761 aaacagaaac aatctgttta agagcagtga gacaaaatgg aatggcatta gaatttgtaa
    26821 aggaacagac tgttggtata tgcttaaagg cagtaagaca atatggaatg gcattaaaat
    26881 ttgtaaaaga acaaacagaa gaaatttgta cagaagctat aaaacaagat aaaagagcac
    26941 ttagttttgt aaaaggagat aaggaaaaat acaaggctct atatgataat aatgaaccat
    27001 ttgcaaagag atatgttaga aatgtaatag aaaaagaaaa cagagcattg attaaaaaag
    27061 gagaagaaaa tgcaaaaatc aaaattgcag gaaaacttta tgatagaggc atgtcttttg
    27121 aagatatatc agatatagtt gagattgaaa taagcaagtt aaaagtgagt ttaggtgttt
    27181 tttaaagtaa taaatgtagg tgttttttat gggaaatagt taataaaaaa ataatcgatt
    27241 agctctttgt aggacagcaa aattagtgat ataatttgat gtataataaa ttatttttat
    27301 tagataaggg ggaattaaag ttgaagatta cagtattata ccaaacaaga agtggaaaca
    27361 cagaaagggt cgctaagctg atagaagagg gcgctaaaaa ggtggatgga gtagatgtaa
    27421 agcttatgag acttgataat attgatttag attatttaaa tgaaagtaaa gcagtaatat
    27481 ttggaacacc tacttattta gcaaatacaa cttgggaagt aaaaaaatgg tttgatgaag
    27541 attcaaaaaa agttaattta gctggaaaat taggagctgt ttttgctaca tgtgattata
    27601 tatgtggagg tccagatgtt gcgatactta ctatagtagg acatttgatg gttaaaggta
    27661 tgcttgtgta ttctggaggt ggttctttag gaaagccatt tatacattta ggacatgtac
    27721 attcaattga aggtccagag ttacaagatg agaaggctat aatatttggt gaaagaattg
    27781 ctactaaggc aaaagaacta tttgcttaat ttctatatgc ataataatga ttagagtaaa
    27841 tttagggttt taagggagat ttgaagtaaa agttataaaa taaaaaattt tccataaaat
    27901 tactcaagat gaattataat ataattataa ataaattata ttattaaaat atttaaaatc
    27961 aaacgtcaat agcgagggaa gtttatggta tggcatatag aatatgagat tttttctgca
    28021 attatagtta tttttttaat ggtatacttt tttagaagta aatttatacc tactttacaa
    28081 aacaaaatat attgtgcact tttaatattc tcatttttat ttataatttc aaatatttta
    28141 ggttcatttt gtctaaataa tatagacaaa attccaattt ttattacttt tttattgaat
    28201 cagatatact tattgttatt gccaatacca gcagcacttg tatcatttta tgttatggct
    28261 attatttatc aagacataag gtatatgaaa aaaagattat tatttttatc tataccactt
    28321 ataatttgta taatcttatc tattacaaat ccttttacgc atattctatt tggattgtca
    28381 aaagaaacag gttatatacg aggttgtgga tatgttgcaa cttttgcatc ttttttcttt
    28441 tatgcaatat attcagcatt tttatctttt aaatatagaa aagcaatgca tgaatcaaaa
    28501 atatgggcta ttagaggttt tcttatagtt tcaattgtgg caattatctt acaggcaatt
    28561 tttttacagt atcttcttac aggaactgca tgtgcatgta gtttactttt gacatatctt
    28621 tctatgcaga atcgaggtct tattattgat gaccttactg gtgtactcaa tagacaatct
    28681 tttattcaag agctagattt gaatatcaat actgaacaat caggatttat tattacaatt
    28741 gcgcttgatg attttaagtt tatgaatgaa acttttggaa caaaaaatgg agatattgca
    28801 ctaaaagaag ttggaaaata tttgattcag atacatgatg taaaccatgt atatagaata
    28861 gggggagata tattttctat tgttgtcaat agtcaattag gtatagaacc agatgatatt
    28921 atcagaaaga tagaagaaag atttaaaaag ccttggctag tagaagagat aagttttaat
    28981 ttagcaacta gtattgctgt tgtttattac ccagaaaatg cagaaacgac agaagatgta
    29041 attgttgcta tagatttctc tatacatgaa gcaaaacgtt ctgagtcaag gcgagttgtt
    29101 tatgcagatg cgtctataag tgaaaagata aagaagaaac atgttattaa aaattgttta
    29161 aaagaagcat taaaaaatga tgggtttgaa gtgtactacc aacctatatt ttctaatagc
    29221 aggggcaggt ttacttcagc agaggcatta cttaggctag aacacaaaga gttaggtttt
    29281 atacctcctg atgaatttat tccaattgca gaacaaacag gattgattaa ctcaattgga
    29341 ttagtagtct ttgagaaggt atgtagattt atagctagtg atgagtttga aaatttaaaa
    29401 ttggataata tagctgtgaa tctttctgta gtacaatgta tgcaaaaaaa cttagctgat
    29461 gatttacttt atcttatgaa taaatataat gtatcaccaa ctaaatttaa acttgaaatt
    29521 actgagactg tggctatggg ctcttttaat ataataaaag aaacaatgga aagattaatt
    29581 gatttaggtg ttaagtttgc acttgatgat tttggaattg cttattctgg agttactaat
    29641 atgctttctt taccattttc agtaattaag ttagataaga gtttgatttg gtctatgaat
    29701 gaagatagta ggcataaact tacagttgag acgattatag cacttataaa taaattgaat
    29761 atgaaggctg tagcagaagg tgttgaaacc attaagtgtg ctgaagattt aatagctatg
    29821 gagtgtgaat atttgcaagg gtattatttt tctaaaccag ttccagaaga tgtttttaaa
    29881 aatttgttga aagaaaatac ttttacaagt taacaccctt tattttttta attacaactt
    29941 tgttcattga cactgccagc gatatattat aaaattaaca caataataaa tattaaattt
    30001 attatacata ttattaatat taattaatgg agtaaagatt tattatatat tttatttaat
    30061 tatcctaaag tgagaaagga agatgtgaca tgaataaaaa gaaaatatta gtgccaattg
    30121 atgggacaga aagaagtatg cattctttgg aatttataaa aggaatattt aaaaaagatg
    30181 aagttgaagt agaaataatg aatgtaaaag aactagtatt tattgatggc atatccctgg
    30241 ctgaagagat aaaaaattct gaaaatttag gaagaagaat cttggataaa gcagcagaga
    30301 taatgggaga gtatgatgtt aaagtacatt ttacttttgg atatccagga gatgagataa
    30361 ttagaaaagc aaaagaagat aatgtagatt ttatagttat gacaaaatct actaaaaaag
    30421 gtcttacaag aatgataggt tcagtaacag caagtgtagt taaacaagca aaatgtatag
    30481 tgatgattgt accggagtag aattagaaag gtatttgaaa tatgaataaa aagatagtag
    30541 cagtttgtga aagtgaaaga agtttaaact ctcttaagac agctgcaaat gatttaggat
    30601 ttgatttagt ttatgaaatt caagaaaatg ataatataaa aaatgaactt tctatttatg
    30661 atatagaaga tgcatccata gtgttatttg ttacaagtga ttctatagaa agcatagaga
    30721 agattgatag atttatagat agagaatttt atgaagttga tccaaagtat ataatagagg
    30781 atgcaaaatc tatagtaagt gaaattttaa tagacttaaa ttaatcgtgg gtgtcgcact
    30841 aagaatagag cgacgccttt ttatatattt tacacaagaa ataaagaggt tgtatcaaaa
    30901 tatagataag actctttttc attgttgaaa tgatgaaaat atatttgatt tcatacaaaa
    30961 aagagtacct cataactaaa aagttcactt tgagacactc catttttata tgttagttgc
    31021 atttaaggta aaatataagt ttaatcatgt tgaaataact gaattacatc ttctaaatga
    31081 ttgtacattg atgtataacc taagtaagag tctttactat caaatgcaat cattatcttg
    31141 ttatggtggc taatcatatc cttgagagaa tttctatatt taaaaccgaa gtattggtta
    31201 gaagaaactg ttttaggtat taactcataa tattttataa aaattggatt atgagaagct
    31261 aaaattatct ctttgtgaaa ttgcaagtca gcatcataac attcttctat caaaccatga
    31321 gaaaccttat ccaattcctc tattgacttt aggtaagtat attttatttt ttgaagttct
    31381 ttagatgtag catttaaaat tgctagttta gtagcttcac tttcgataag ttgtcttgct
    31441 cttaaagatt ctacataaaa ttcttgtatg ttatgatttt tatagatttc accatcatca
    31501 aataggtcaa cttttttaaa tccttcaact ataggacttg aataaggcac agctctaagt
    31561 attccttcct tacagagtat atttatggca tttcttacag gtattctact tatgttaaat
    31621 aggtcagcaa tctttctctc acttggtaat ttactattaa ttttaaaagt tccatttaga
    31681 atgtttaatt taatataatc aataattttt tcagtggcat ttaagctttt cataaaaact
    31741 cctaaacata taattttttt atgattgtat tatatcatag ttaagagtta aatctacttt
    31801 tttataatat gtataagaca ggatagaggc caaaatagct ataatagcca agataaagta
    31861 tccatattgt attgaagtat attccgatat tttaccaact atcattggaa atacagacat
    31921 tcctatagca taaattgctt gaaaaaaacc cattgcagta gatttctgat gttttggtac
    31981 atttttcata gactcgctag taaggtaaga aaggagtata cctgtagata tacctggtag
    32041 tacttgtagt acaaatatta tattaatatt ttttattgtt ggaactaaaa agcaataggc
    32101 tgaaactaaa ataaaaacta aaggaatcca aaattttggt ccatacttag tacaaaattt
    32161 agtagaagca aagccagaag aagcaacagc acacaccatg taaaatatag aagatagacc
    32221 tatgagggta gaactagaac ctaagttttt aagaatttga gtagtaaatg acattgttgt
    32281 agacatttga ataccctgtt gaattaatgc cataaaggaa aaaaatacaa gtcttttatc
    32341 cttgcagata gataaatatt ttttaattga tattttttta ggtatttttt ttgaattatg
    32401 attttttata tttaaggcta gaatcgttcc aataactgaa gacaatacac tcatgataca
    32461 taaggctgtc attcctaatt tttcataaaa taaagtactc ataacaaatc caatcaacat
    32521 tcctagattg tttaccaata ttatcgtact tgtagctttt tgttgatgtt ctgtagaaaa
    32581 aaagttggta tagtgaacca tgtaagaaat ccaagttgat gatgatattc cagataaaat
    32641 gttagctata agaaaaccat tgcctccagg agaaaagact cttataagag atgctaaacc
    32701 agcaaagaaa attccagaca gtataaatat tttatgttta tttatagaat cagcaaatac
    32761 tccaacagga agccttaata aacattgtga gattccatat gcacctatta ttataccaat
    32821 catatttgta gatacattac atgcacttaa atatggattt tggaaaggta
//
LOCUS       DAEMGG010000032        32614 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEMGG010000032 DAEMGG010000000
VERSION     DAEMGG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.032"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            75..542
                     /locus_tag="KPK24_001482"
     CDS             75..542
                     /locus_tag="KPK24_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299163.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            585..2348
                     /locus_tag="KPK24_001483"
     CDS             585..2348
                     /locus_tag="KPK24_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG2299164.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            2356..2964
                     /locus_tag="KPK24_001484"
     CDS             2356..2964
                     /locus_tag="KPK24_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2299165.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            3036..3605
                     /locus_tag="KPK24_001485"
     CDS             3036..3605
                     /locus_tag="KPK24_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG2299166.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            4123..7662
                     /gene="nifJ"
                     /locus_tag="KPK24_001486"
     CDS             4123..7662
                     /gene="nifJ"
                     /locus_tag="KPK24_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG2299167.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(8054..8950)
                     /locus_tag="KPK24_001487"
     CDS             complement(8054..8950)
                     /locus_tag="KPK24_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG2299168.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PGNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPEDNNNSDSTNKPD
                     DNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKKSQDMIDNNYF
                     SHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFS
                     KIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(9446..10780)
                     /locus_tag="KPK24_001488"
     CDS             complement(9446..10780)
                     /locus_tag="KPK24_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2299169.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            complement(10842..11618)
                     /locus_tag="KPK24_001489"
     CDS             complement(10842..11618)
                     /locus_tag="KPK24_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG2299170.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            complement(11723..12388)
                     /locus_tag="KPK24_001490"
     CDS             complement(11723..12388)
                     /locus_tag="KPK24_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG2299171.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            complement(12388..13044)
                     /locus_tag="KPK24_001491"
     CDS             complement(12388..13044)
                     /locus_tag="KPK24_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG2299172.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIIEGGNL
                     "
     gene            complement(13053..14231)
                     /locus_tag="KPK24_001492"
     CDS             complement(13053..14231)
                     /locus_tag="KPK24_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG2299173.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTITVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQSKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            14586..15488
                     /locus_tag="KPK24_001493"
     CDS             14586..15488
                     /locus_tag="KPK24_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2299174.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            complement(15704..16516)
                     /locus_tag="KPK24_001494"
     CDS             complement(15704..16516)
                     /locus_tag="KPK24_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2299175.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(16519..17478)
                     /locus_tag="KPK24_001495"
     CDS             complement(16519..17478)
                     /locus_tag="KPK24_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2299176.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            complement(17584..19143)
                     /locus_tag="KPK24_001496"
     CDS             complement(17584..19143)
                     /locus_tag="KPK24_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2299177.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            19362..20339
                     /locus_tag="KPK24_001497"
     CDS             19362..20339
                     /locus_tag="KPK24_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299178.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            20340..21311
                     /locus_tag="KPK24_001498"
     CDS             20340..21311
                     /locus_tag="KPK24_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG2299179.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKKIKSVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            21463..23505
                     /locus_tag="KPK24_001499"
     CDS             21463..23505
                     /locus_tag="KPK24_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBG2299180.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNIMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVIKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATILLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            23766..24608
                     /locus_tag="KPK24_001500"
     CDS             23766..24608
                     /locus_tag="KPK24_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2299181.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            24911..26983
                     /locus_tag="KPK24_001501"
     CDS             24911..26983
                     /locus_tag="KPK24_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2299182.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVSTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(27196..28065)
                     /locus_tag="KPK24_001502"
     CDS             complement(27196..28065)
                     /locus_tag="KPK24_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2299183.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            28229..29683
                     /locus_tag="KPK24_001503"
     CDS             28229..29683
                     /locus_tag="KPK24_001503"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG2299184.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEAYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            29954..32233
                     /locus_tag="KPK24_001504"
     CDS             29954..32233
                     /locus_tag="KPK24_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2299185.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
BASE COUNT        12105 a         4104 c         5234 g        11171 t
ORIGIN      
        1 ttttttatta ttaccaagta taaaaaatat tataattgta aaacttacaa atatatgtaa
       61 aatatttagg agtgatgaaa atgttaaatg ggataataaa gcttttttat tctgaaattg
      121 taaaaacaaa gattaataac ttagattcaa gtggatattc tttaagagat gaatgtaaag
      181 aaatcagaaa tataactcta aagatagata gtgtattttt taagctttta aagttagctt
      241 ttggttcaat attgcttatg gtagctttta atttaaacat ggttttaggt ataggagtgt
      301 ttttagttgg tgtagtttat atactatata aaagaaatat ggaagaacaa gtaaaagagc
      361 atataaataa tgtgaaaaat aatatcgaca tctctaaaat aacttttatg gatgaaaaag
      421 ggagaacagg tctaaatgca ttaattacat tacttcttat aggattattg acaggattta
      481 actggatatt agttgctagt tttgtcgttg tttttgtttt tactataaag aatgtatgtt
      541 aaaatactga taaatagttt aatatatagg gggagaaatt gagtatgact aatggcagtt
      601 ataaaatgaa gaatttagtg gaaatatccg ctatggtgac tgaatctaca gattttttta
      661 atataaaaga tgacataata gaaaaaatgt tagaagttgt tcaccctaca aaggcatgtg
      721 tgaatctatt ttataaaaat gattctaaat atgcctactt agtatgttcg caaaccttag
      781 aatatgtacc tcaaattttt ccaataaata gtcttaaagg tgcaaaaatt gattttgaca
      841 catatccaga gtatatacat gaggctgtaa atgaaaaaaa aataatatat gtggaaaatg
      901 tatttgaaga ttctagagca gaaggtgaac gagatttagc aaaagctgaa ggttatattg
      961 ggagaatagt ttttcctttt ataataaata acgtagttgt tggatttatg acttgttttt
     1021 taaaagagga tgattatata acagaacaag atatagattt tatatcatct gttgcatcac
     1081 tcttaagttt atcaatagaa ataactaata aaaataataa tgtacaaatt ttaattgata
     1141 aactaagagg ctcaataagt gccataaatg aagctactaa aaaactatac cttaataaga
     1201 gtattgatgg atttttggaa aatttaagta aacaagcgtg taatattact aatagtaaag
     1261 aagctttaat aattataaat agtcatcata ataaggatgc tatagttagt tgttataatg
     1321 ttgagcaaaa aaagaaaaca aatttatatc ctatgattga tatgttttta aagaaagagt
     1381 cattgggggg gtatttaaat aatttaaaac catgctcaaa aaagggaata aatttggaaa
     1441 gttatatata ttacaaactt caggataaaa atgagactat aggatgtata gtttgtgcaa
     1501 atagcaaaaa ctatactagt gatgatttaa atattcttag tatattgtcc aaacaagttt
     1561 ctgttggtat gcaattatat gaacataatg aaaaggaagt aaaacacaaa gttcttgaaa
     1621 acgagttgaa catattaaat aaacaacaac agcttattat ggataaaggt aaaatggatt
     1681 gtaatgatga gaaagaactt tatttttacc ataaaccagc aagggtagta ggtggagatt
     1741 tttattatgc tacaaaagtg gatgatgaac atatagttta tattgttgct gatgtaatgg
     1801 gacatggaat ggtatcaaat tatgttgttg caatgataaa aggtgctttt aaagtattat
     1861 gcaatcaata caatacacca agagaaataa tgacaaattt aaataggatg ttatatgatg
     1921 aatttgacaa gatgggagta tttacaactt gtttgatagg tattatagat actaaagaaa
     1981 atatgattac tgtgtctaat gcaggtcact atagtcctgt agttgtaaag aaagatggca
     2041 caatagaaaa taatttaaat tgtaaaaagg gcatacctat tggaataatg gaagatgcta
     2101 cttatgaaaa taatactctt tcaatggaag attatgcaat ggtatgtatg tacactgatg
     2161 gaattttaga gataaaaaat tcatcaaaag aagagtatgg tataaacaga ttagaaaact
     2221 ttctaaaaga aaattttaga ttaaatcaac agttaatagt agaaaatcta aaattggatt
     2281 taaagaactt ctcatcaaaa gataattatg atgatgatat tttaatagtt atgttaaagg
     2341 ataggtagat tgattatgga ttcaaacgat aaaaaaggac ttaaaagtcc agagattata
     2401 ataataaatg gaagtccaag aattaataaa aattgcagtt ttatagcaaa tgaagtaaaa
     2461 gctatgctag aagagtgtgg attaagttgt aaaatattta atatctacaa tatggatatt
     2521 gaatattgca cagcatgtgg attttgtgaa aaaacagggt attgcaagtt taatgatgat
     2581 atgaagccat tatataaaat gtttgatgat tcaaaaggaa ctatagtaat aagtcctgta
     2641 cattttgatt caatatcagc taaattaaag actgttgttg atagaactca agctatatat
     2701 gcaagtaaat atgtattaaa taaaccttca attgatagaa gtaagaaacg gataggaatg
     2761 tatattgcag tgggagggag taaatcatat gattcacagt ttaaaggtgg acagattgtt
     2821 atggattttt tctttaagag tataaatact aaacttttac acaatatata tttaaacaat
     2881 acagatgatg tatcttatat agataatcaa gaatttaaag atataatata taaaaattta
     2941 aagatatata ttgattcaat ataatttgat tttgtaggta atattatgta taattgtatt
     3001 gcaataattt aaataattaa aaggagggaa tttcaatgaa attaccaata gcattatcta
     3061 ataaacatgt tcatttgagt caagctgata tagacgtatt atttggagca ggtcatgagt
     3121 taactaaatt taaaccatta tctcaaccag ggcaatatgc ctgtgaagaa aaagtggatt
     3181 tagtaggacc aaaaggaact ttaaaaggag ttagagtact aggaccagct agagctcata
     3241 ctcaagttga aatatcttta gcagatggtt tcccattagg agtaaaagca ccaataaaag
     3301 aatctggtga tatagctgga actccaggtg ttaaattagt aggacctgct ggagaagttg
     3361 aaatgaaaga aggagttata gttgcttcta gacatataca tatgagcaat gaagatgctg
     3421 ctaaatttgg tgtaactgat aagcaaatag taaaagttaa aacatctgga ccaagagcat
     3481 tagtatttga aaatgtatta gtaagagcta gtgacagctt taatctagaa atgcatgttg
     3541 atatggaaga gggaaatgca gcaggcgtta aaaatggaga tttagtagaa ttaatagttg
     3601 attaatatta taaatagact ttatgctagt agatatttta tttgctagca tatttttaaa
     3661 tgtaataaat ttctatagga taaatttaat caattagatt aatttataaa ataaaggggt
     3721 attataaaaa atgtaataaa aacttacaaa aatatgatgt aatatggtaa aattatatat
     3781 ataaaaattt acatgtaata aggataagca ttttactttt tatactaata tgaacaaata
     3841 gataaaaaag tttgaaaaat ctaacttaat ttattatata ttgtatacaa aattatgaaa
     3901 aaaacttaac atatttaatt ttaaaaaaaa tctacttaaa tatgtaatta atttacaatt
     3961 ttcaaaggaa ttttatctaa tctgtagtat tatattagaa gatagagtat ttgcgcgact
     4021 atatgtgtgt ttatagtttt gttgtgtaaa ttatatgtaa atataaaaaa atagacttgt
     4081 tataaacaag tacaattgta gctatcaagg aggatacata ttatggctaa gtttatgaaa
     4141 acacttgatg gaaatacagc tgcagctcat gttgcctatg cgtttacaga tgtagcagct
     4201 atatatccaa tcacaccatc ttcaactatg gctgaagtag ttgatgagtg ggcatcacaa
     4261 ggaagaaaaa atatatttgg acaaacagtt aatgttgttg aaatgcagtc agaagcaggt
     4321 gcatcaggaa cattccacgg ttctttacaa ggaggagctt taacttctac ttttacagca
     4381 tctcaaggtt tattattaat gatacctaac atgtataaag ttgcaggaga gttgttacca
     4441 ggagtattcc atgtaagtgc tcgtgcttta gcatctcaag ctttatcaat atttggggat
     4501 caccaagacg ttatggctgc tagacaaact ggatgtgtat tattagcttc tggttcagtt
     4561 caagaagtag ctgatatagc acctgttgca catttagctg caatagaagg tagattacca
     4621 tttatacatt tctttgatgg attcagaact tcacatgaaa tccaaaaagt tgaattacta
     4681 gaaaatgaag attatgcaag tttattaaac tttgaagcag ttcaagcatt tagagataat
     4741 gctttatctc caaatcatcc agttgctcgt ggtactgctc aaaacccaga tatatatttc
     4801 caaactagag aagcttctaa taaatactat caaaatatag taggtatagt tgaaaaatac
     4861 atggaaaaaa tgagtaattt aacaggtaga aaacatagtt tatttgatta ctatggagca
     4921 gaagatgcta aatacgtatt aatagctatg ggttcagtaa cagaaacaat agaagaaact
     4981 atagattact taaatgctaa gggagaaaaa tatggtttag ttaaagttca tttatataga
     5041 ccattctcaa tgaaacattt cttagatgct atgccatcta ctgttgaaag aatatgtgta
     5101 cttgatagaa ctaaagagcc aggttcaact ggtgaaccat tatacttaga tgtacgtgat
     5161 gtattctatg gaaaagaaaa tgctcctatg ataataggtg gaagatacgg attaggttca
     5221 aaagatacta ctccttcaga tataaaaact gtttttgata acttagtttc agaacaacct
     5281 aaaaatggat ttacagtagg tatagttgat gatgtaacta atacttcttt aactccatct
     5341 gagccaataa aaatagcttc taaaggaact ataagatgta agttctgggg attaggttct
     5401 gacggtactg ttggagctaa taaacaagct atcaaaataa tcggtgacca tactgaaaaa
     5461 tatgctcaag cttactttga ttatgactct aaaaaatctg gtggtataac tatgtctcac
     5521 ttaagatttg gtgatactcc aataagagct acttatttaa tagatgaagc tgattatatt
     5581 gcctgtcata aacaatcata tgtttatcaa tatgatttat taaaaggtct taaaaaaggt
     5641 ggtacatttg tacttaacac tatatgggac caagcaggac ttgaagaaca tttaccagct
     5701 catatgaaac aatatatagc taaaaatgat ataaaattct atacagttaa cgctgttaaa
     5761 ctaggacaag aaataggtct tggaaacaga ataaacatga taatgcaatc tgcattcttt
     5821 aaattagctg aaataatacc agaagaagat gctgttaaat acttaaaaga ttctatagtt
     5881 aaagcttatg gtaaaaaagg tgaaaaaata gttaacatga actatgctgc tgttgatgca
     5941 ggaataaatg ctttagttaa agttgaagtt ccagcttctt gggaaaatgc tgttgatgca
     6001 gataaaggag aagttaaaga accagaattt ataaaaaata tactaagacc aatgactgca
     6061 caagaaggaa acaacttacc agtaagtaca ttcaatggta tagaagatgg aacattccct
     6121 tgtggtactg cagcttatga aaaacgtggt atagctgttg atgttcctga atggataata
     6181 gataactgta ttcaatgtaa ccaatgttct tttatatgtc ctcattcatg tataagacca
     6241 gtattagtta ctgaagaaga attagctaat gctccagaag gatttgaagc taaaaaagca
     6301 ttaggtaaag gattagaagg attaaaatat agaatgcaac ttagtccact tgactgtaca
     6361 ggatgtggaa actgtgctga catatgtcca gctaaagaaa aagctttagt tatgaagcca
     6421 atagatactc aacttgatac agagttagat aattgggcat ttgcagtaaa tcctgatgag
     6481 gtagctccaa aaggtgatgt tatgcctgca aacactgtta aaggaagtca atttagacaa
     6541 ccattaatgg agttttcagg agcatgtgct ggatgtggag aaactgcata cattaaagta
     6601 gttacacaat tatatggaga tagaatgatg atagctaatg ctactggatg ttcttctata
     6661 tggggagctt ctgcaccatc aattccttat acttgtaacc atgaaggtaa aggtccatct
     6721 tgggcaaact ctttattcga ggataatgct gaatatggat ttggtatgta cacagctgtt
     6781 aagcaaataa gaaataaaat agtagatgct atgacagagt tagtttctat ggatatctgt
     6841 gaagatgcta aagcggtatt tacagagtgg ttagattcta gaaatgatgg agaagcttct
     6901 aaagtagcaa gtgctaaggt tgttgaatta ttagaaaaac cagcttgtga ttgtacagat
     6961 gaaaaagcta aagagttagt aaaagctata aaagatagaa aagattatct agttaagaga
     7021 tctcaatgga tactaggtgg agacggttgg gcatatgata tcggttacgg tggattagac
     7081 catgttcttg catctggtga agatgtaaac gtacttgtat ttgatacaga agtatattca
     7141 aatacaggag gtcaagcttc taaagctact ccagctgctg ctatggctaa gtttgctgct
     7201 tcaggtaaaa aatctagaaa gaaagattta ggaatgatgg ctatgacata tggaaacgta
     7261 tatgtagctc aagttgctat gggtgctgat aaaaaccaat ttatcaaagc cgttatagaa
     7321 gctgaaaaac atgatgggcc atctttaata atagcttatg ctccatgtat aaaccatgga
     7381 ttaaaagaag gtatgggaag aactgtagct aacgaagcac aagctgttgc ttgcggatac
     7441 tggcatttat acagattcaa tccagaagta aaagctgaag gtaaaaaccc attcacttta
     7501 gattcaaaag aaccaactgc aagcttcaaa gatttcatac ttgcacaagt aagatattct
     7561 gctatagcta aacaattccc agaggaagct gatggattat ttgctaaagc tgaagctgat
     7621 gctaaagaaa gatatgaagg atacaaaaaa ttagctgaat aatattaaag tattataatg
     7681 cttatataga attgataaaa gagagtgtct caaagtgagc tttttagttc atgaggcact
     7741 ctttttgtat gaaatcaaat atattttaat catttcaaca atgaaaaagg ccttatctct
     7801 attttgagac agcctttttt tatgaagata ttaagatcct gtttttacct attaaatatc
     7861 tttttaagtt ttccattttc atcttgccaa tactttttat atattgctct acacactaaa
     7921 attaagagaa caaataatat ttatagtatg tatatttgga tttgataatt ttaaaataga
     7981 tacaaaagag tatctcattt gtgtttttta tcacttagag ataatctttt gatatgtatg
     8041 gaaaaagatt attttatcct acaaagattt gtgtccaata aattgaacca ccactttttt
     8101 gtgctactcc aacacctatt ttactgaaat ttggattcat tatatttttt ctgtgacctt
     8161 cagaattcat ccatgcattt acaacttctt taggagtttt ttgacccata gctatatttt
     8221 cacctgctgt cttatagctt attccaaatt ttttcaacat gtcaaatgga gaaccataag
     8281 ttggagaatt gtgtgaaaaa tagttgttat caatcatatc ttgagatttt tttgtagcaa
     8341 catttgatat agaagaatca agagtaagag gatttaatcc agctttagct ctttctatat
     8401 ttactaagtc aacaacttct ttttggtaag ctgaaaaact ttcacttgta gagcctgagt
     8461 tgttattatc atcaggtttg ttagtagagt ctgaattatt gttgtcttca ggtttatcag
     8521 tagagcctga attattgttg tcttcaggtt tattagactc aggtttatta ttatcaggtt
     8581 tattagtaga gcctgaatta ttgttgtctt caggtttatt agactcaggt ttattgttac
     8641 caggcttatt agtagagtct gaattattgt tgtcctcagg tttattagtg ttgttgcaat
     8701 tcttgtcatt atgattctta tccttacaag agttatcttt atcaggctta agttctggtt
     8761 ttacagaaca atttggtttt gcagaaaact tgtatttagt attactatat actaaacttt
     8821 tgttacttat atttacatta ttttcaagtg atgaagcact tgcagtaaca gcacttgaag
     8881 ctgttgctaa agttaacact cctaaagcta atatagtttt taattttgtt ggtatatttc
     8941 ttctcatcat taaacacctc ttaatctaaa aattctaaaa atgttactga tttgtaacct
     9001 ttagtgtagg gggttacccg tgaatctata ctaacataag attaagactt tttttactgt
     9061 aaagatatgt aaaaaagata tatatggaat aaataggtat atttcagtgt gtttgacaat
     9121 ttgtattaaa agcctgtata gtatggaatt acttagttag agaaaataat atttttataa
     9181 taattccata acgtaaattt atagttatga taaatttttt tttatttggt taaatttatt
     9241 cagtagatgc tttcgtatta gcaacaaatt ataattattt aaatctttaa gaaataaaaa
     9301 gggtctgtct caaaatagag ataaggcctt ttttattgtt taaatgatag gcatatgtta
     9361 tttcatataa aaagagtgtc tcattaagta aaaagttcac tttgagacac cacctttcat
     9421 attatgaatt ttaatttgtt ttatattaat acattagtaa tcctaatgta attacaataa
     9481 tttctgctat tagtgaacca actgcaacta caatgaaagt ttctttaaat ccagttttat
     9541 gtgttaactt agataagctt aagaatgtaa gaaaaacacc actttgtgga actatagtta
     9601 gtatatttga agctattgta gcaacacggt gaattaattc tggatttaga ccagtactta
     9661 aataataatc tgcaaagttt ggcattacta ttcctaatgc accagaagag gaccctgtta
     9721 ttgctgacat tgttgaagtc aaaacagtaa gacttataag tggactacct ggtattgaca
     9781 ttataagttt gctaaataca tcaaaaccag gtgcagccat tattactgct ccaaatgcaa
     9841 ctgcagaaga tgtagcaaat ataggaccta tagaaccact agcacctatg ctcaatgtct
     9901 gtatcttaga tgatagaaat ttatggaata aaaaagcaga taaaagaatt gcaactatta
     9961 aagctatgta aattacattt tttttcataa attctgaacc aaaagtactt ccagttatag
    10021 ctataattac gagtgctaat agaggaaaaa tacttgcaaa aaatcctggt aattctctat
    10081 ctaaagattc ttcttctaat tctgaagtat aagttgcata agtttcacct tttctaatac
    10141 ttttgtttag agcatatttc atatagacaa gtccaaacgt agcacaacct atactaccta
    10201 caatacttgg tatagtagct gcagtaagag acgtatttag atattgaata ggtataacat
    10261 tttgaatggc tggtgttcca ggaattatag tcatagtaat tgttgcaata ccaagccaaa
    10321 gtggtatctg aataaggttc caagaaatat caagtttttt aaatagagtt cttgcaagtg
    10381 gaatgaccgc aaacataact acaaataagc ttataccacc atacgttaat ataaaactta
    10441 ctataaatat agcaactaaa actctataag gatgattaga gccaaattta tttaaaataa
    10501 aatcagctat tgatacagta gctccacttt cttccataaa tttagcaagt attgagccta
    10561 ataagaatat tgcaaaataa ctcattatat agtttccaag tgcacccata taattgtttt
    10621 tgtcagcacc tagcattgat gttacaacat ccattttatt taaaataaca actattaaag
    10681 ttgctattgg agcagctata gttatatgaa tttctttaac tgaaaaatat ataatagcta
    10741 tgataccaat aattatacca attggtgcga taatactcat aaatatccct ccttagtaga
    10801 aggacctgaa gaatactttc ttcaagccct aaatataata actattgtgc agtatatcct
    10861 ccatctagta agcaagcttg tcctgtaata cccttagcat tatcacttgc taagaacata
    10921 gcatagtcgg caatttcttg aacatctaat aggcgttttt gtggtacaag tgggtataaa
    10981 acttcctcta agacattttc caatggtata ttacgagttt ttgaaaggtc attgaactga
    11041 cctctgacaa gtggagtatc tacatatcca gggcataaag cattaactgt tataccagaa
    11101 tcggcagctt caagagcagc aacttttgtt agtccaataa gtccatgttt tgctgagtta
    11161 taagcagcct ttccagcaaa tcctattaca ccattgattg aggccatatt tattatacga
    11221 ccaaagcctt gttttttcat tataggaaat gcttttttag tagctataaa aggtgctgtc
    11281 agcattattt ttatcataaa ttcaaattta tcagttggga aatcttctat catagaaaca
    11341 tgttgaagtc cagcattgtt tattaatata tctaatctgc catacttttc aacagttttg
    11401 tctatagcgt ggttaatttc ttcttcctta gttacatcac actttacact catgcaatca
    11461 taaccttttt tttgtaactc tgttatggta ttatttaaag cttcttgatt tatatcagag
    11521 aacattactt tagcaccatt ttttaagaac gattcagcta tttgtttacc tattccactt
    11581 gcagctcctg ttacgaatac aactttatct ttaaccattt tcatttcctc caattttatt
    11641 ttatttttat attaatattt taagcaatac aaatttatat gtaatacgtc taagagattt
    11701 ttattttcta gttttaatta ttttaaacaa cagatatttt tccatttaat gaatctgaca
    11761 atattaaagg agcctcagta gcagaaataa catcatctaa tgtgaaagct ggatttattt
    11821 ctttaagtaa aattccatct gatgttactt ccataactcc catttcagta actatcaaat
    11881 ctacagcatt tgttgcagta agtggaagag tacaattatt taatattttt gcagaacctt
    11941 ttgaagtatg ttccattgct acaataactt ttttagctcc agttactagg tccatagctc
    12001 cacccatacc tggaaccatc ttacctggaa tcatatagtt tgctatatta ccatatttgt
    12061 caacctgaag tgcgccaaga acagttatat caacatgacc tcctctgatt attccaaaag
    12121 aagtagcact atcaaagaaa catcctccag gaagtatagt aactggttga ccacctgcat
    12181 ttacaatagt ttcatcactt gaatttccat cttcggctga acctaaacct aaaaagccat
    12241 tttcagattg taagataacg tttacattat ccggaatata atttgctact ttggttggta
    12301 gacctattcc aaggtttaca acggctccgt cttttaattc tttagatact ctatttgcaa
    12361 tatattcttg catttctaat ttatccatta taaattacct ccttcaatga tgtaattcac
    12421 aaaaatacct ggtgtattaa cttcatttgg gtcaatatca ccaatttcaa cgatttcatc
    12481 tgcttcaact attgtaattt tggcagcaga tgccattaaa ttattaaaat tgttcataga
    12541 gcctttataa actagattac ctgctttatc gacctttgat gcaaatagaa gagcaacatc
    12601 tgcataaatt ggtttttcaa gaaggtattc tttgctatcg acaacaattt tttgctttcc
    12661 ctcctcaaca agagtaccaa taccagtcgg tgtgagtata cctcctagac cataaccagc
    12721 tgcacgtatt tgttcagcaa gagttccctg aggaactaac tcaacatctg tttcattgtt
    12781 attcatttgt cttccagttt ctttatttaa tcctacatga gaagcaatta tctttttaaa
    12841 ttgcttattt acaaccattt tgccaactcc tttgtcaata aagccagtat cattgcaaat
    12901 tagtgtcaaa tcttttatat ttttactaca taaataatca attaaatttt ctggtgaacc
    12961 attggccatg aatccaccaa ccattattgt atctccatct tttacatgag atagagcttc
    13021 atcaatgcta actattttat tcatatttcc tcctatcgtg aaaccaccat tgatattcct
    13081 tgaccaccac caatacaaag tgttgcaagt ccagtttcaa ctttacgttt ttgcatttca
    13141 taaattaagg taacaagtat tctagctcca cttgctccta ttggatgtcc taatgctatt
    13201 gcaccaccat taacatttaa ctttgatgaa tctatttgaa gttcattttt aacagctaat
    13261 gcttgggcag caaacgcttc atttgcttct attaagtcta tatcattgat gctaagtcca
    13321 gcttttttca gtgcttttct agttgcaggg attggaccag tacccatgac ttcaggttca
    13381 acaccagctg aagcatagga ttttatttta gcaagtggtc taatacctag ttcgtctgct
    13441 ttttgttggc tcattagtat taacatagcc gcaccatcat taatacctga ggcatttcca
    13501 gctgttacag taccatcttt tttgaatgca ggtttaagtt tagctaaacc ttcaaatgtc
    13561 atacctaatt taggatattc atctttatca atagtttcta tttttcctcg acgtattaaa
    13621 acatctactg gaacgatttc ttctttaaag cggttgtttt ttatagcatt ctcagcctta
    13681 ctttgacttt ctaatgccaa tttatcttgc atttctctag taaattcaaa tttagttgca
    13741 atattttctg cagttattcc catatgatac tgattgaaag catcaataag gccatctgtt
    13801 agcatactat ctaccatggt tatgtcaccc attttactac caaagcgagc agttggaacg
    13861 atataaggag cttggctcat attttcagta ccacctgcaa ttaccacatc attttcacca
    13921 gatgtgattg attgggcagc tagctggaca cttttaagcc ctgaaccaca taatttattt
    13981 actgtgtagc ttggaacaga atttggtata cctgcattta tagcaatttg tctagcaaca
    14041 ttctgtccaa gccctgtttg aagaacattt ccaattatta cttcatctat ttcagataaa
    14101 ttaagtccta cacgtgatat cgcttcttta acagtgatag ttccaagttg aacagctgaa
    14161 gtatttttaa atacacctcc aaaacttcct ataggtgttc tgactgctga tacgattact
    14221 acatctttca tatctaatag ttacctttct tgaatgataa ttaaaaattt tctgaataaa
    14281 gaaattagga aacactataa taaagtactt ttttaataca agcttgtttt taagtatttc
    14341 aattactttt attcctaatg ctttaggttt tcctgtttct aattatagat tacggcgaaa
    14401 aaagcgtttt gtctaagtct aattttttat atagtgtata agaaaaattt ataggataaa
    14461 aatttatagt agatttagaa aaatattttg ttaaaatatt aatgaaatta tgttgatttt
    14521 cctataaaat atcattagaa gaaaaaatat aatctaaatt atacttttgg ggaagggggt
    14581 ttaaaatgga tataaaacag ttgaaatatt ttgttgaaat agtgaaatct ggttttaatt
    14641 tatcaatagc atctaagaca cttcacatat ctcaaccagc attaagtcaa attataaaaa
    14701 attttgaaga aagtgaagat gtatatttat ttgaaaggta taaaggcagg ttaaatgggt
    14761 taacaccagc aggagaaaga ttttatgtaa atgcagaaca tatcataaat gaatacaaaa
    14821 atatgatgga agatttaaga gaagattcag ttcaatttaa aggaaaagtt agaataggaa
    14881 tacctccact tatacttgga atagcatttt cagatgtagt tgcacagtta gttgcaaata
    14941 atccagatat agaatttgat atagtagaga agggtgcata tgacttaaat agaatgctta
    15001 tcttacagga attagattat gcagtattgt tacatccaca taaaattgat aaaagtgtag
    15061 taactgaaca tgtgcttcaa gaagatgaac ttacagcatt tttaaatata aatcatccac
    15121 ttgctaagaa ggataagata gattggaacg atttgaatga gcagttactt gcaatattta
    15181 atcctacttt tatgatacat cataagctaa tgcaaaaatt tactgatgaa aatgttaagt
    15241 taaaaagata tataatgtct ggttcatggg attttttgtt gctatcaacc acaaattctg
    15301 attttataac aattctacca tcaccagttc atgatttttt tgaaaatagc aaaattatag
    15361 aaaagccttt taatcatcct attacatgga aagttatatt atgtagacct aaaaaagaac
    15421 gttatagcca tgtagaccaa catgtattta aatttattat agatttcttt agtaaaaaga
    15481 actcttaaaa atgttattaa aataacaaat aaataaatca atttttatta taataaacta
    15541 ttagtttttc atctatatgt ttgagagcaa taataggttt tacataacat ggtagagtag
    15601 tatttaaagt tataatatac aaaatatgta tttttttaaa cgaaaaataa aaacagtgta
    15661 tatctttaga taatcactgt ttttattttc tatataaaat ttgttaagta ttactagaaa
    15721 attttttgct tattatagtc aaactcatga ctgttaaaaa tataagtaat cctggaaata
    15781 cagctaaata taacttgtca aaaatatgtc tttgagcatc ttgaagcata ttaccccatg
    15841 atgcaagagg caattggatt ccaagaccta aaaaactaag agcagattca gttaagatag
    15901 cattagcaat atttaacgaa ccagcaacta caatagtctc ttttaagttt aaaactatat
    15961 gtcttaatag tatatttcta gtcttgatac cagtagcttt agcagctaga cagtattcat
    16021 tttcctttat ttttaatgtt tcgccacgta caattcttga aacttccatc caacttaaga
    16081 atccaataat tagtataata gtggaaactt taggtgttag ataagtattt aatacaatca
    16141 aaactaagaa acttggtata ctcataagca tatctactaa tctcatcaag aattggtcta
    16201 gcttacctcc aatatatcca ctaataacac ctacagcagt accaaatact acagaaataa
    16261 gcattgataa aataccaaca ctaagtgaaa tccttccacc ataaagagct ctagagaagt
    16321 aatctctacc aagttcgtct gtaccaaaga tatgctttaa gctaggttct tgaagtttag
    16381 aagtagtatc aattgcatta gagtctatat ttaatataaa agaagctata attaatatga
    16441 ttattatgct aagagtaata catgcaaaag caaaggattt atcttttttt aattttttta
    16501 agatacttgt aaacacaact agaatcctcc tttgctagat tttattttag ggtcaattaa
    16561 taagtatagt atatcagcaa ctaaattacc taaaattaaa aatagtgctg ataacatagt
    16621 tattgccata attacagtat aatctcttga aaaaattgaa tcaatcatta atcttcccat
    16681 acctggccaa gagaaaacta cttctgtaac aaatgcacca gtgactaatt tttgtatact
    16741 catgcctagg attgtaacca atgatgtcag tgaatttttc aagacatgtt taaatagtat
    16801 gactttttct gataatcctt ttgctcttgc tgtaataaca tattgttttt ttaattgttc
    16861 atttacacta gcttctacat aatttgtaaa aatagcagta ttatatagac ctaaagttat
    16921 tacaggcaaa actaaatgtt ttactaagtc tccaatacta tcatttccaa tagtatgcat
    16981 acctcctgat gggaaaatgt taagtttaac tgaaaaaatt gaaatgagta tcatacctat
    17041 ccaaaaagaa ggtatagaaa cacctatata attaaaaaca gatgatactt tactagttat
    17101 tgaattttta tttacagcta aaaatattcc aagaggtata gctaccaata ttgaaaatat
    17161 caaagaagta cccataagaa taaatgtatt tcccattttt tccataattt gttctgtaac
    17221 gggcatatgg tttatataag aaatacctaa gtttccactt aaagtgtttt tcaaccagtt
    17281 taaatatctt atatatactg gtttatcaag ccccatatta acccttatat tttcaatatc
    17341 ctgtgaagac attaaaggat ttatatatgc atctgctggg tcaccaggag ctaattctaa
    17401 aagtccaaat gataatatag atattaagat taacataggt attaaaactt ttactctatt
    17461 caatattaat tttaacataa gtaaattctc ctaattaaaa gagatgtctc gttttaaaat
    17521 aataaaacaa tattattttt agacatctcc ttttctaatt aataatataa aattttacat
    17581 caattattct acatacagtt tagaccaatc ttcaaataat attgcaggag atggtttagc
    17641 atcttttatt ccttttagat ttttttgagc tgccattaaa ttttgttcat aatctatagt
    17701 gtaaataggc gcatccttag atatatcctg ttgtatttgc ttatatattt cttcacgttt
    17761 ttccttgttt aactcagtaa atcctttttc ccataaagca tctatttcag cattagaata
    17821 tttagtttgg ttgcaatatt gaccagtttc aaacatacct ctatatgggt ttggttctaa
    17881 tccccaagca taaccattta catacatatc acattcagtg ctattactaa atagtatatt
    17941 gaagaatgcc ttactttcat aaggaactat ttcagcttct ataccaattt ttttcaactc
    18001 ttgttgagct actaaggcat agtttttatg cccaaatcta cctgtgttat aacctatttt
    18061 taatttatca attttaacac cacttttatc caataagtct ttagccttat ttgtatcttg
    18121 gtcatatcct tcaacacctt cttctgtata gaagtctgct tctggaacta atatagattt
    18181 agcaggtaca gaaccttctt ttccatatgc tgattctatc atttcatttc tatttaaggc
    18241 atatgataag gcttgtctta cttctttgtt agccatgttt gatatatttt ggttaaatac
    18301 aatgtagttt aatctttctt cactataagt ataagtttgt aagtttgagt ttgattttgc
    18361 cttttcatat ccttcagcag atgtcttcat aagtgaaatt tcgccattat ttaaagctgc
    18421 ttcttgagat gcttcattag gaattatttt tagcgctata gaatctgcct taggttctcc
    18481 accaaaataa tctgcattct tttcaaatac tatgctttca ccttttttcc attccttaaa
    18541 cttgaatgca ccagaaccaa ctggattatt atttttttct gacttagcta tatttgattc
    18601 tccttcaaac acatgttttg gtattggaga aattttagat ataccatata agaaagattc
    18661 acttgcagtt ggtaaagtaa tcttaactgt taaatcatca attttttcta ctttaactgg
    18721 tttctcaccc accaataagt attgtcttga tggtgaattt tgtttttcgt ctaaaactgt
    18781 attaaatgta aataagacat cttcagcagt tattggcttt ccatcatgcc attttaaatt
    18841 ggattttaat tttatagtta attccttact atctttaaaa tctacttttt ctgctaaata
    18901 atttactaaa tttccttttt cataagtata aagaggagca taaactatat ttgatgcaag
    18961 agagtcttct ttaacgtttg caaaagctcc attgattata tctgggtcac ttgccatagg
    19021 tattacaaca gttccaccag atttgcttcc actagacttg tctttatcat tggaagcaca
    19081 ggctgtaaat gtaagcatta atgatgaaac taagattaat gatgccaatt ttttaaattt
    19141 catattctta taaaacctcc ataaaataat atttaacaca tattttttat tgtataatag
    19201 taatcattat taaatcaata tatatagaaa ttaactatta tattattata attaatagaa
    19261 aaaaataata aaatgtaaga aaatgttgta atttaaattt ttataaaata caatcaaaag
    19321 gtgtatgtta tcttaagtaa ttttgctgag aggagaaaat aatgagtaaa gcactaattg
    19381 aggttaaaga tttaaaagtt tattttcata cagataaagg gattgtaaag agtgtaaatg
    19441 aagtaagttt taatataaat gaaggtgaaa ctattggaat agttggagaa tcaggatgtg
    19501 gtaagtctgt aacagccatg tctttgatga agttacttcc aacatcaaaa atagaaggtg
    19561 gagaaataat ctttcgaggc aaagacattt taaagatgaa tgaagatgaa cttatgggaa
    19621 taagagggaa tgagatatca atgatttttc aagaaccaat gacatcttta aacccagcgt
    19681 ttactgttgg aagtcaaatc attgaaggta ttatgataca tcaagattta tcaaaggaag
    19741 aagctaagaa aaaggtaatc gatatgataa aactggtaga aataccaaga gctgaagaaa
    19801 tatataattc atatccacat gaattatcag gtggtatgag acaacgaata atgatagcta
    19861 tggctttatc atgtaatcca aaattactta tagctgatga accaacaaca gctttagatg
    19921 ttactattca agctcagatt ttagatataa tgaaaaatat aaaagaaaaa ttaaacacat
    19981 ctataatgat gattacacat gatttgggag tagttgcaga aatgtgtgac aaagtattag
    20041 tgatgtatgc tggcaaaatt atagaagtgg cagaggttgt tgaattattt aaaaatccaa
    20101 aacatcctta tactattgga cttctaaaat caaagcctgc tcttggaaaa aacaaagata
    20161 aaagattgta ttcgattcca ggacaagtac caaatccaat tggaatgcca gattcatgtt
    20221 attttagtga tagatgtgaa aaagtatgtg ataaatgtag aactcagata cctcctttaa
    20281 tagaattgaa tagtggtcat agtattgcat gttggttata taaaaaggag gagatataat
    20341 tgaaaaaaga agagagttta ttaaaagtag aaggtcttaa aaagtacttt gaagttaaat
    20401 ctggattatt tgataagaaa attaaatctg taaaggcagt agatggaatt tcttttgatt
    20461 taaaaaaagg agaaactttg gctatagttg gagaatcggg atgtggaaaa tcaactgcag
    20521 gaagaaccat tcttagatta attgaaaaaa ctgaaggtaa agttgaattt aatggtgtag
    20581 atatatataa gttagagaag gaagaactta gaaagttaag acccaaaatg caaatgatat
    20641 ttcaagaccc atatagttca ttaaatccaa gattaaatgt ggaacaaata atatccgagg
    20701 cagttataga acataattta gtgccaaaga atgaaataaa agataatata ttaaatgttg
    20761 tagaaagttg tggtctttct aagtaccatt taaatagaaa accaggtgag ttttctggag
    20821 gtcaaagaca gagaataggt atagcaagag cattagcatt aaatccagaa tttatagttt
    20881 gtgatgaacc tgtttctgca ctagatgttt ctattcaatc tcaaattata aatttactta
    20941 tggatttaca ggataaatat aaactttcat atttatttat atctcatgat ttgagtgtag
    21001 taaatcatat tgcacataga gtttgtgtta tgtatctagg ttctataata gaaattgcaa
    21061 ctaaaggaga actttactta cattctcaac atccatatac aaaagcactt atgagcgcag
    21121 taccaataca agaccctaca attaagaaaa atagaattat attaaaagga gacataccat
    21181 ctccaacaaa tccaccaagt ggatgtaagt ttcatacaag atgcccttat tgtaaggaaa
    21241 tatgcaaaaa agagattcca gttttaaaag aaatatcaag aaatcatttt gttgcatgtc
    21301 atctagtata gatatttcaa aaattaaatt tgatgtatat aataagaata atagaataaa
    21361 ctgaaaattt ttttgattaa gatgggattt catgctgtta atattaagtt agttgatata
    21421 taagtttaat ctataatatt attattgaaa agagaggtag ttatgtcaat tttagtaaca
    21481 ttgttgggat tattggtttg tacaacgatt tcaactgtat ttagcaaaaa gtggagtaat
    21541 attcctcttg caatatatca aattgtacta gggattatat tgtctatttt gccatttaaa
    21601 ttctcatttt catttaatcc agaaatattt gtaatatgta tcatagctcc attactattc
    21661 agtgaaggac aaaatgtatc gagaaaagag ctattagagc ttagaaaacc aattttgctt
    21721 cttgcatttg gtttagtttt aataaccgtg tttgcaggtg gaatctttat acattttctt
    21781 attcctggga tgccattatc tgtttcattt gctttggcag cagtaatatc tccaactgat
    21841 ttagtagcag taaaatcaat tacacaagga ttaaattttc ctaaaaatat aatgagtata
    21901 cttgaaggtg aatctttatt aaatgatgct gctggagtag tagcatttaa ggttgcagta
    21961 cttgctacag ttacaggagt tttttcaata gaagaggcag ggattcaatt tatgattaca
    22021 gcttttggag gaataatagt aggaagtatt ttaggataca taattataaa aataagactt
    22081 agtttacaca agtggaattt ggaagaaatt ccaatggtaa tagttataca aataatgact
    22141 ccactttttg tatattttgt agcagaagaa ataggagtat ctggaatcct agctgttgta
    22201 atggcaggga tagctcatgg aattgaaaaa gagcatttac aaaatacaac tactaaatta
    22261 aggataatat cagacaatac ttggtatgtt ttggagtatg tattgaatgg atttgtcttt
    22321 acactgcttg gttttttgtt accaagtata tatacaggac ttagtagtaa aaatgaaaat
    22381 atggcattgg aattgacttt tatttctact ttaattgtac ttattttatt tgttataaga
    22441 tttatatggg tatatttatg gcataattct tttataaaac ctaaaaagaa tcctttaaat
    22501 aatttttttg taggtttttt aggttttaag gatgaagaag ttataaaaga gagtatttct
    22561 aaatgtaaat actcactaat agttgctact tgtggtgttc atggtacatt tacactagca
    22621 actgccttat ctataccttt ttatttggct gataaagatg tatttccaat gagagatact
    22681 gttttattta tatcatcaga agttattctt ataagtttag tattggcaac aattttactg
    22741 ccaagattat taaaaaataa cgtacaagag tcagaacctt tattatctga taaagaggca
    22801 tataaactaa ttttgaaaga agccatttta aaacttagta atgaaaaagc acaagaagta
    22861 caaccagtta tacatgattt aaatgaacag ttggtagact cagaaaaagg aatactaaat
    22921 actccagata ataaacttat aagtatgatg attgcagaag cagaccaaca gggattatta
    22981 gctgttttga aactattaga agatggtaaa atttctgata gagcctttaa attgtataga
    23041 ttttatatta caaaatcaag aaaatctgtt caaaaatcta tatttaaaat tgtaaaatta
    23101 aaaataagca tgtggattat aaatagaaaa gtaaagaaag aagtaaaaga aaaaattaca
    23161 aaaatagtat ctgagaataa agaaataatc aaagagtttc accatgcata tgcactttct
    23221 actaaaactg ctatatcatt tataaataaa aatactgatg aagataacag acatgaggcc
    23281 ttaatagcaa tcagattcta taatcgttat ttgaattgga ttactagaaa gattcaaaat
    23341 gatgataagt ttgaggcaag agtagaatac tacagagtaa tggcgataca aagtcaaaga
    23401 gacagtgttc aacaattagt tgaaactaag caaatatcaa gagaagtggc taaagagtta
    23461 ttggaaaata ttctctatga tgaaatgatg atgatagaat cataaatata atggaataaa
    23521 atttgatttt tgatgttaaa attaatacta aaaaatagga aatagttttc aataatttac
    23581 ttgacatata tatttattta taatagaata aaaccataaa ttgaatacaa tatataagca
    23641 ttaacaagct ggattgttac tggtaagcag gcaataccta ggttaaggta tacaggtgaa
    23701 ttgtatctta atttaggtat ttttttatga ataagggaaa ttgtattttg ggagagatat
    23761 tgattatgat tattcaacaa atttataata ataatgttgt attagtttta gatgaaaata
    23821 taaaaaaaga attaatatta acaggatgtg gaattgggtt tcaaaaaaag aaaggtcaag
    23881 aaatagataa aagtaaaatt gaaagaactt ttgtaataca agatgaaagc tttttagata
    23941 aaattagtaa actagcaagc caggttgatg agaaattttt tgaggttagt actgaaataa
    24001 ttgcttatgc agaggagaac ttgaatacta agctttatga atatatttat gtagcactta
    24061 ctgaccatat tgcatttgca attaaaagat atcatgaaaa cataactata aagaatgatt
    24121 tactacatga gataaaaaga atacataaaa aagaatatga gattgggaaa tgggctgtag
    24181 attatataaa taaagaattt gatgtgaaat ttccagtaga tgaagctggt tttatagcaa
    24241 tgcatatagt aaattcaaac tataagggaa gttcaaagga atcactatta attactaaaa
    24301 ttgtaaaaga catactaaat attataagat attattatag agttgaattt aaagaagatg
    24361 atataaatta tgataggctt ctgactcatt taaaattctt tgcaaaacga ttagtaaaaa
    24421 aagaaaaaat caatgataca aataatgaaa ttatagatat aattaaagta aagtatgaaa
    24481 aggattatga ttgtgcatat aaaataaaaa ctcatgtaga aaagaattat gattactatg
    24541 taagtcaaga tgaactactt tatttaacat tacatatcaa aagagttata tctgtactga
    24601 atttataata aagagtagtt taatacagaa tgtatagatt taatatatta tgtttaattt
    24661 taatatatta tttaaaattt aatataaaat aataatttta aaacattaag attgttactg
    24721 ataatgcagg caagacttat gtttataaaa aaattataat agtttatttt taattctgaa
    24781 attaggttaa tataggttta tttatattaa cataagttta attttagaat taagtatact
    24841 aatgtaactt tttataaact taagtctttt ttatttaaaa aaatataaaa taataaggag
    24901 agaaaagtaa atgaaaaaag tatttggtgt tttacaaaaa gtaggaaaat ctctaatgct
    24961 accagttgca ttacttcctg cagcaggtat attacttggc gtgagtaatg ctttagcagg
    25021 agacgcattg ttggctcatt ttccagcttt ggcgaatcct acatttcaac ttataataag
    25081 tataatggaa agttcaggtc aaattatatt taataactta ccacttatat ttgcagttgg
    25141 ggtagctgtt ggtttaactg aaggagaagg tgttgcagca ttggcagcca tagtaggttt
    25201 tttaattctc aatacatcta tgggtgttat ggcaggtgtt acagaagata tggtagacaa
    25261 ttctatgtat gcaaatattg ttggaatacc aacaattcaa acaggggtat ttggtggcat
    25321 tattataggg atagtggctg cttatcttta taaaaagttt tataaaattg aattacctca
    25381 gtatctaggt ttttttgcag gtaaaagatt tgtaccaatc gttacttcat tagttgctat
    25441 agcaatagga attatattgt cttttatttg gcctccaata caagagggat tacttagttt
    25501 ctcaaaaaat gttatagaag caaatcaaac actagcagca tttatggaag gttctttgga
    25561 aagactatta ataccatttg gtttgcatca tgttttccat aatccatttt ggtatcaatt
    25621 tggagaatat gtaaataaag ctggtgagct tgtaatgggt gaccaaaaga tattctttgc
    25681 tcaacttaaa gatggagtaa atttcacagc aggtactttt atgacaggtg catttccatt
    25741 tatgatgttt ggtcttccag cagcagctct tgctatggtg catgaagcaa agccagaaaa
    25801 gaaaaaattt gtagcaggta taatggcatc agcagcatta acctcttttt taacaggaat
    25861 aaccgagcca atagagtttg cgtttttatt tgttgcacca gtattatttg caatacattg
    25921 tttattttca ggtttatcat acgcaattat gcaattatta ggagttaaaa ttggattcac
    25981 attttcagga ggattaatag atttcttatt atttggtgtt cttcctaata gaactccatg
    26041 gtacttggct atcttggtag gtattggttt tgcagtatta tactatagtg tatttagatt
    26101 tatgataaga aaatttaatt taaagacacc aggaagagaa gatgagtcag aaactaacgc
    26161 tgaagtcatt gaaatgtcta agaatgaatt agcatcagag attttgattg ctttaggaaa
    26221 taaagacaat ttaaagagcc tagatgcgtg tataacaaga cttagattgg ttgtaaatga
    26281 tataaataaa gtagataaag aaaaattgaa atcacttgga gcagcaggtg ttatggttat
    26341 tggcaacaat gttcaagcta tatttggacc aaaatcggat tcaataaagt ctgatataaa
    26401 agatataata gagggaaaaa caaaggttga aagtactgtt actaaaaatg ttgataaaaa
    26461 taaagtgaaa aaaaatatgg aagttgcaaa tatggaaaat gctttagtag aaaagattga
    26521 ttcattaaca aatggagaga tattagatat agaagaagtg ccagattctg tattttcaag
    26581 taaactgatg ggggatggat ttgcaattaa atctagtgat ggattgattt attcaccagt
    26641 tgatggaact attggagtta tatttccaac taagcatgca ataataataa aatcccaaaa
    26701 aagtggagta gaaatactta tacatttagg gatagaaact gtaaatttag aaggaaatgg
    26761 atttgaagta tttgtgaatg taggggatga agtaaaagcg ggagaaaaat tagttaaaat
    26821 ggacttagaa tatattgaaa aaaatggtct atcaacaata agtcctgtag tatttacgaa
    26881 tttagaacaa aatcagaaat taagcattaa aaaaggagta agcacagcaa aagaggagaa
    26941 tagagtaagt atattaagag atgatattga aaaaatagga tagctatatg atagtgtaac
    27001 taaaagttat gtgggattta ataaatatat aaataggttc agttaacatg caatttattt
    27061 aatatatatt ttaagtaata gaaatatata tcattatatt ttgatatatt ttaaaacatt
    27121 aaaaaacttg aggtattagt aagataaata cactaatact tcaagtttta ttaaaataaa
    27181 atttacataa attatctatt catctaaaag agtaatatcc aaaggatttt tattgtttat
    27241 ataattttta ggagataccc cgtagttttt tttaaatgcc cttacaaaac tagaataatc
    27301 gttgaatcca catttattac aaacttcact cataaggtga ccattggtta ttagtgactt
    27361 tgccaaaatt aatctttttt gaactatata attgtgtata gacgttccag tatgagattt
    27421 aaattttctc ataagataat acttacttat aaaaaattca gacgatatgc tatctattga
    27481 aagcttatca tctatatttg aatttatata gtttaatact ttcttaatag tttcatcata
    27541 aaaaacatct tctgttgatt ccatattttc attttttagg aaaattctgt ttaagaatat
    27601 cataagttgt ataaataatg catttctcaa tatttcattt ccaaattcag tattggattc
    27661 ggaaatattt atttgctcaa taaaactctt aatagattca atatcatgca tatttaaacg
    27721 caatctattg catttattct ctgtagctat tttaaaacat ttttctaagt cactactagc
    27781 atcactattt tttttcataa attctgggct aatccatatt gcaattctct cataaaagac
    27841 atttgaatct ataactggtt tatggatttc attattgctg ataaagagta tatcccaagg
    27901 ctttagttta tagggaatac catcaacaaa ataagttaca tttccattta taaatataat
    27961 tatcttatta aaatcatgat tatggtattc aaattgtata tcttttttat ctctaatatt
    28021 aaatatttta aagttatcgt ttaagtaccc gattttatta tccatagttt ttatagaaga
    28081 cctttcctca gttatattat agaaaattat agagcatttt ttgcaatgtt tcaagcattt
    28141 ttaaccattc acatagtaaa aaattcttaa tataatatta tatgtagtgt aattttagca
    28201 gaaagagttt aaagggagga aagcatttat gaaattaaat gatattatac aggggttgga
    28261 tataatcaat gtcaagggtg aattaaatat agatattaat aatgtacaat atgattcaag
    28321 aaaggttaca aaaggtacat tatttatatg tataaaagga tttgtgtcag atggtcataa
    28381 gtatataaaa gatgccatag aaaagggagc aagtgcattt cttgttgaag aagatgtagc
    28441 aataaaagga tgtacattta taaaggtaaa agatacaaga aaagatatgg caaaagtagc
    28501 tgataatttt tacaatcatc catctcaaaa atttaacgtt ataggagtta caggtactaa
    28561 tggaaaaact agtataacta ctatactaaa tgagatatta actttaaata agaataaagt
    28621 tggacttata ggaactataa aaatttttga tggagaaaaa gacattgttt ccaattcaac
    28681 aactccagaa agtatagatt tacaatatca ttttaataat atgttggata atggatgtga
    28741 ctactgtgcg atggaagttt catcacattc attggcatta aatagagttg atgaaacaga
    28801 ctttaaacta ggcatattta caaatttaac accagaccat ttagattttc ataaagattt
    28861 agaggcttat agaaaagcaa aagaaaaatt attttttaag actactatgg caaatataat
    28921 aaacatagat gatgaaggtg gaaaaaagat atatgaaaat ataaaaggta taaatgtacc
    28981 ttgttacact tatggtgttg atactaaggc tgattttatg gctagagata taaaatcaga
    29041 ttcggatgga gtatcttata gattaataac accttcatat gaagaagtaa tatttatacc
    29101 agttccagga atgtttacag tatataatac tttagcagtt atagctgctt gctatgtact
    29161 tggcatacct aaaccaatat ataaagaagg ccttagactt tctaatggag tatcaggaag
    29221 gtttgaaaca gttccaaatg ataaaggtat aagcgtaatt gtagactatg ctcatactcc
    29281 tgacgcactt gaaaatgtac taaagacaac tcaacaattt gcagaaggaa aaataatttc
    29341 tgtgtttggg tgtggtggag atagagatac tgaaaagaga cctttgatgg gagcaattgg
    29401 acaaaaatat tctgatttat gtattataac ttctgataat ccaagaactg aagaaccaga
    29461 ggcaataata aaagatatac ttgaaggaat tgacaagaaa aaagaaaatt accatgttgt
    29521 tgttgataga gaacaagcta tagcagaagc tataagtatg gcaaaaaagg atgatgttgt
    29581 aattataaca ggtaagggac atgaaactta tcaaattata ggtaaagtta agcatcattt
    29641 tgatgataaa gaagttgcaa atgagtgttt aagtaagatg tagcataaaa attaaacaaa
    29701 atacaagaac aaaaatgtaa tatcaagctt cactttgata ttacattttt ttatatgtaa
    29761 ataattgtaa aatagataat gcaataatgt aaacactaat atctatatta attaatttat
    29821 tgttgtaata aagtttataa ttttgtataa ttacagtaaa tatggaaaaa taagtattat
    29881 tataaaatgt ttacaataat tgtcataaga taatgattat acatacatag attcgagaaa
    29941 ggcaggaagc ataatgttaa gtgatgcaaa aaataatagg tatggtttgt ccataattgc
    30001 tgtcataagt atcttagtaa ttacaatttc ctgtttgatt tatggttcaa ggttgaataa
    30061 gacattaggt gaagaaacga atcaatatct ttcagaaata gcaaatcaaa gcgtcaatgt
    30121 gttaaaaaaa caaattaatg gagatataaa gttactagag tcaatttcaa tatttattga
    30181 aggagaagag tcttttgaag tagataatat cctttctata ctaaaaaggc aagctattaa
    30241 tagttcattt aaaagaatgg gagttatttt accaaatggt acagcataca ctacagatgg
    30301 ttatatagaa gacttctcta aaagagatta tttcttaaaa tcaatgcaag gagaagtggt
    30361 tataacagga aaattaaaag atgtaataga agatgataaa aataccaata gtaatattaa
    30421 tgtatattct gtaccaattt ataaagataa tgaggttaaa ggagttatat ttgccacaca
    30481 tagtacgaag ttgtatgaaa aaatactttc tgtacctaca tttaatggaa aaggatattc
    30541 ttatataact agaagtaatg gtgatattgt tttaaatcca agcagtaaaa acgccaactc
    30601 tactaaagaa aatttatttt cttacattgg cagaagtgat tcaatatcaa aatctagtat
    30661 caacaatatg aagtcaaata taaaaaactt taaatcaggt tcattaagtt atagtatgtt
    30721 tggaaaagga tattatttaa gttatgcccc aataggaatc aatgattggt atcttttttc
    30781 tgaagttcct aaaactgcta tatctgagaa atcatatgct attataaaac ttacattatc
    30841 agcatgtgta gtcttgatta taatctttac ggcattaaca atttatatac tatttataca
    30901 taaaaagagt aaggaaaaat tggaagaatt tgcttttaaa gataacgtta caggtattgg
    30961 caattcaaat aaatttaatc ttgaaggggg aaaattttta tcctctcatg agaaaaagaa
    31021 tctagtttta atttattttg atatagataa gtttaagcta gtaaatgata ggtttgggta
    31081 tgaagaagga gatagagtat taaaggaaat agcagagata attaagaata tatttaaaga
    31141 gcaatctgta ttttcaagaa tttcaaatga caattttgct ataatatttg aaaagaaaaa
    31201 taatagagag tcaattatag aaatatgtga acttatacgt aaaaaattgt caatgataaa
    31261 aacaagtttg ggagtagaac tcaatttaat tccatccata ggtgtatatt ttgttgaaga
    31321 gggagaaaca aatatttcta cctgtcttga caaagctatg attgcaaaaa ctacagtaaa
    31381 aagaaaatat ttaaaagttt atgagattta tgctgaaaat ttaaaagaaa ctctcataga
    31441 agaacgtgat attgaacaag aaatgcatga tgctttaaaa aatgaacaat ttaaagttta
    31501 tttacagcct aaggttgatt tatcaactac taaaattgta ggtgctgaag cacttgtacg
    31561 ttggcaacat cctaaaaaag gtctaataag tccaggtgta tttattccta tttttgagaa
    31621 aaatggcttt ataacagaat tggatatgtt tgtatttacc caagtttgta aaaattttaa
    31681 acgttgggaa aatgaaaatt tccccacatt tcctatatca ataaacttat caagagtaca
    31741 tcttgagaat ccaggcttta tttctgagct tgaaagaata acaaaagaat atgaaattga
    31801 accaaattta attgagattg aattgacaga aagtgcaata tttgacaata caaaaatatt
    31861 atttaaaata atgcaaactt tgaaatctgt tggatttaaa atttctatgg atgattttgg
    31921 ttctggttat tcttctttaa atatgcttaa agatatgcca attgatgtat tgaaattaga
    31981 taggcaattc tttataacag tgggaaatgc aaaaaagagt caaattgtag tttcaagtat
    32041 tgttcaaatg gcaaagcaac ttgatataaa agttgtttca gaaggtgttg aaactgtaga
    32101 gcaagcagag tttttaaggt ttataggttg tgatatggct caaggatttt tatttgctag
    32161 accaatgcca atagaagaat atgaaaaatt aatattagat agatatagtt taaatagaaa
    32221 agaaacaaat taaataaaat tgtgaattat aacataaaaa tttataaaaa agtgaacttt
    32281 gttaatcaat gttcactttt ttatgtaaaa atatactatt ataaaaacat aaatcattgt
    32341 taggatatta ttttaggaat tattgggcaa agtttattaa ttatgtagta ttttttctat
    32401 ttgaatgttt ttgaggtaag agtatatggg attatagcaa aatactttag atagtaataa
    32461 tagtaatttc tataggtgtg ttatacatct tttaaaaaat aaaaagataa tttataaatt
    32521 taaatgttat atatgtaagt aattttatta tatcaaaaaa agagtttttc ataataattt
    32581 atacaaatca tttgaaatac tcttctttta tttt
//
LOCUS       DAEMGG010000033        32514 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEMGG010000033 DAEMGG010000000
VERSION     DAEMGG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.033"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(270..986)
                     /gene="dapD"
                     /locus_tag="KPK24_001505"
     CDS             complement(270..986)
                     /gene="dapD"
                     /locus_tag="KPK24_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG2299186.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1210..1959)
                     /locus_tag="KPK24_001506"
     CDS             complement(1210..1959)
                     /locus_tag="KPK24_001506"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG2299187.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(2026..2913)
                     /locus_tag="KPK24_001507"
     CDS             complement(2026..2913)
                     /locus_tag="KPK24_001507"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG2299188.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(3030..4031)
                     /locus_tag="KPK24_001508"
     CDS             complement(3030..4031)
                     /locus_tag="KPK24_001508"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG2299189.1"
                     /translation="MKKVNVAIIGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4456..5337)
                     /locus_tag="KPK24_001509"
     CDS             complement(4456..5337)
                     /locus_tag="KPK24_001509"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG2299190.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGIISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            5660..6853
                     /locus_tag="KPK24_001510"
     CDS             5660..6853
                     /locus_tag="KPK24_001510"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG2299191.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            7059..8177
                     /locus_tag="KPK24_001511"
     CDS             7059..8177
                     /locus_tag="KPK24_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG2299192.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            8308..8553
                     /locus_tag="KPK24_001512"
     CDS             8308..8553
                     /locus_tag="KPK24_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG2299193.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            8540..9286
                     /locus_tag="KPK24_001513"
     CDS             8540..9286
                     /locus_tag="KPK24_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2299194.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKSEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            9571..10446
                     /gene="hslO"
                     /locus_tag="KPK24_001514"
     CDS             9571..10446
                     /gene="hslO"
                     /locus_tag="KPK24_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG2299195.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            10837..11637
                     /locus_tag="KPK24_001515"
     CDS             10837..11637
                     /locus_tag="KPK24_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG2299196.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            12164..14404
                     /locus_tag="KPK24_001516"
     CDS             12164..14404
                     /locus_tag="KPK24_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2299197.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            14818..16353
                     /locus_tag="KPK24_001517"
     CDS             14818..16353
                     /locus_tag="KPK24_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG2299198.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            16369..17130
                     /locus_tag="KPK24_001518"
     CDS             16369..17130
                     /locus_tag="KPK24_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299199.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            17468..18337
                     /locus_tag="KPK24_001519"
     CDS             17468..18337
                     /locus_tag="KPK24_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2299200.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEVARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            18666..18917
                     /locus_tag="KPK24_001520"
     CDS             18666..18917
                     /locus_tag="KPK24_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG2299201.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            19029..19808
                     /locus_tag="KPK24_001521"
     CDS             19029..19808
                     /locus_tag="KPK24_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2299202.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(19943..20593)
                     /locus_tag="KPK24_001522"
     CDS             complement(19943..20593)
                     /locus_tag="KPK24_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299203.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(20590..21093)
                     /locus_tag="KPK24_001523"
     CDS             complement(20590..21093)
                     /locus_tag="KPK24_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2299204.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(21142..21657)
                     /locus_tag="KPK24_001524"
     CDS             complement(21142..21657)
                     /locus_tag="KPK24_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG2299205.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            21937..22461
                     /locus_tag="KPK24_001525"
     CDS             21937..22461
                     /locus_tag="KPK24_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2299206.1"
                     /translation="MHEQIDCCERKKRTIDIKGENMRELLKWVSIINRYNTNYIDRKL
                     ADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVK
                     DKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMN
                     EEAKLEAKENGRKI"
     gene            22445..23848
                     /locus_tag="KPK24_001526"
     CDS             22445..23848
                     /locus_tag="KPK24_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2299207.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(24412..24723)
                     /locus_tag="KPK24_001527"
     CDS             complement(24412..24723)
                     /locus_tag="KPK24_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299208.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(24779..25372)
                     /locus_tag="KPK24_001528"
     CDS             complement(24779..25372)
                     /locus_tag="KPK24_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2299209.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            26223..26873
                     /locus_tag="KPK24_001529"
     CDS             26223..26873
                     /locus_tag="KPK24_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG2299210.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEYIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            27076..27747
                     /locus_tag="KPK24_001530"
     CDS             27076..27747
                     /locus_tag="KPK24_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2299211.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            27744..28874
                     /locus_tag="KPK24_001531"
     CDS             27744..28874
                     /locus_tag="KPK24_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2299212.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLIDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            29305..30150
                     /locus_tag="KPK24_001532"
     CDS             29305..30150
                     /locus_tag="KPK24_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299213.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            30150..31178
                     /locus_tag="KPK24_001533"
     CDS             30150..31178
                     /locus_tag="KPK24_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2299214.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            31181..32194
                     /locus_tag="KPK24_001534"
     CDS             31181..32194
                     /locus_tag="KPK24_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2299215.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVF
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
BASE COUNT        12120 a         3804 c         5395 g        11195 t
ORIGIN      
        1 tcactttatt aaattataaa aagtttaata gtttattaca ttgatatatt gaaaaattga
       61 aggaaataaa aatacttgtc agcaagaaat aaaaatgata taagaatgaa taagagaatt
      121 gtaaaaaaat aaaatgatta taaataaaag ataaaaatat tgtaaaaaag agggtgcctc
      181 tggtgaatta aattcacaca gagacacctt tttaattaat tttttaaatt agtatattat
      241 tgtttaatta atagcatagt actatttaat taatctaaat ttctaaggtc ttccataagc
      301 ttagttttgt cagccgtttt ttcatctttc atttttataa ctttagcagg agaaccagca
      361 acaacggcac cagcttctac atcagtagtt acaacagcac cagctgcaac aacagcacct
      421 ttaccgattc taacaccttc aagaataact gcattagctc ctattaaaac atcatcttca
      481 actataacag gagtagcaga aggtggctct aaaacaccag caacaacagc accagcacct
      541 aagtgaacat ttttaccaag agttcctcta gctccaataa cagcattcat atctaccata
      601 gaaccttcac caataacagc tcctatattt ataacagctc ccatcattac aacagcattt
      661 ttttctatag taaccatgtc tcttattatt gcacctggct caattcttgc atgttcactt
      721 aaagtatttt ttagaggaat agcagaattt cttctatcat attctacatg agtatctgtt
      781 atatcatttc catgtttatc taatacttcc attatatcct catagtcacc tacaaatata
      841 taagaaccat tacttccaaa taccttaaat ttatcagttg attggaagtg taaagagtta
      901 gtatttacat ataccttaac aggtgttttc tttttaactt cttttatata ccttgctatt
      961 tcataagcat cgtttaaatt taccatttgt taattctcct tgtatttcat tatatttctt
     1021 ttattatata cttatttgtc aattttagta tatacttact tcttattatt tatattttat
     1081 tttagttagc ttttattgta tagacatagt attaaataaa tttattatat atgtttgatt
     1141 tgtattgcat attattatat atatattgta tatgtttaat ttatattgtg tattattata
     1201 tttatactat tatcccaaca tgctgtccat attataaaat cctggttttt gcttaactaa
     1261 gtatttggca gcagctatag aaccacttgc aaagatacca cgagattgag cttgatggct
     1321 tattgtaaga acctcattgt ctccagcaaa tagagcatca tgttcgccaa caatatttcc
     1381 acctcttata gcatgaatac caacttcatt tggttgtctt tttgcactac gaccatatct
     1441 accataatta ttttcaacgt ttggcaaaac ctcttttata gcgttagcaa tcataacagc
     1501 tgtgccactt ggggcatcta cttttctgtt gtgatgtttt tctattattt ctatatcaaa
     1561 tccttctagt aatttagctg catctttaac caattttact aatacattta caccaagaga
     1621 catattatat gattggaata caggaataac tttagccgct tcacgtattt tgttcatttc
     1681 gtcatcatta tatccagtag tagcaattac taaaggtgtt ttagtcttta aaacgtaaga
     1741 taagatatta tctaaattag agtgatgaga aaaatctata actacatcag cttcctcaac
     1801 tatagtactc atatcttcat aaattttaag gtcgccttca tagctatcca gacctctagc
     1861 tgcaacacaa accaattcta attcattatc ttctcttata gtatcggcta aaatcttacc
     1921 catttttcca tcataaccag ttgctataac ttttaacata atttcctcct atgattaagg
     1981 gctaactttt gaccaaactt actttttacc atgcattaat ctttactatg cattaacttt
     2041 tagtccaaag ttaactaatt ctttctttaa tacttctaaa tttgctgggt ccatttctgc
     2101 tagaggtaat cttaaatctc caacattgaa tcccaataaa ttcattgcag tcttaactgg
     2161 tataggattt acttctataa ataaagctgc gattagagca tccataccaa gttgtaattt
     2221 tctagaacct tcaatatcgc cttcaaagaa tttagctacc aaatcgtgag tttcttgagg
     2281 gcaaacatta gcaagtactg atataactcc ttgacctcct aaagataata aaggaagtat
     2341 agtatcatca ttaccagagt agatagcaaa gtcatctgga actaaactag ctatttctgc
     2401 aacctgtcct aggtctccac tagcttcttt tacagcaact atgttatcaa ttttagcaag
     2461 ttcaactata aggctaggac ttatatttac acaagttctt ccagggacat tgtaaagtat
     2521 aattggaagt ttaacactgt tagctattgt ttcaaaatga cgcttaagtc cagctttatt
     2581 agctttattg taataaggtg ttattattaa tagagcatct actcctaatt tttcagcttc
     2641 ttgacttaag ttaactgagt gcatagtatc attagaacca cttccagcta taacaggaac
     2701 tcttttatta actttttcaa caacatactt aatagtagca agttgttctt catcagtcat
     2761 agtattagct tcaccagtag taccacaaga tactatagca tcagtaccat tagctatttg
     2821 gtattcaact aattcaccta atttgtcaaa atcaactttg ttatcctttg taaatggtgt
     2881 aactagagca acagcagaac ctttaaatat catataaaat caatcccctc ttttttattt
     2941 ttgtgtttta tattacagtt acttctaatg cttgtttttg agttactatt aatattttac
     3001 aaaattatat atttatacaa gtttgtatat tatactaaat cgtatgataa agccatttca
     3061 gctatttgaa ttgcgttagt tgcagcacct tttcttatat tatcagcaac aacccaaaga
     3121 tttacaccat tatcaacact aaaatctctt cttattcttc caacaaatac ttcatctcta
     3181 tcatcaaatt ctacagcagt tggatattcg tttttagcag gattatcaac tactacaaca
     3241 cctggagctt cttcaagtgt ttttactaaa tcatctaact caaatggatt gttaaactca
     3301 acatttatag attcactatg accatttttt actggaactc ttacagtagt tgcagttatt
     3361 tttaaatcgt agttatcaag tattttcata gtttcattta ccattttcat ttcttcttta
     3421 gtgtatccat tttcagtgaa tgaatctata tgaggaagac agttgtaaga gatttgatga
     3481 gggtagaatc cagtaggatt accgttcatt cctttctcta aatcttcaac acctttaaca
     3541 ccagaaccag atacagcttg gtaagtagag taaacaactc tcttaatttt gtatttatca
     3601 tcaagagctt taaggggaac catagcttgt atagtagagc agttaggatt agctattata
     3661 cccttatgat tttttatggc ttctggatta acctcaggaa ctactaaagg cacatctgga
     3721 ttcattctcc attgactaga gttatctact actaagattc catttgaagc agctattgga
     3781 gcatactttt cactaatact tccaccagca gagaataatg ctatttgtat gcctctatca
     3841 gtaaatgaag tttcctttaa ttcttctact atatattctt taccacaaaa cattacttta
     3901 gaaccagctg atttagaaga agagaataaa aatagatttt ctattggaaa atttctttct
     3961 tctaatactt ttaaaaatgt tctacctacc attccagtag caccaattat tgctacattt
     4021 acttttttca tataaattca ccccaatttt aaattattaa aacattattt tattttagtt
     4081 atacaagtaa gatatgttaa tatttttata tatccaaaaa ctttaatatt atattaacaa
     4141 ctttaattat atacaaaaaa cgtacaaagg tatactttgt acgtctaatt tatcactcaa
     4201 agtaaaccct gtagctcatc acctacaaaa ataagtgaca gtattacaca tgttatgtgc
     4261 aatcccagaa aatattattg caagaaatat tttcttcggc tgtaaatcct ttttccaatc
     4321 attaacacct gccagctact attggatact cttagttaca gctacctcta ccctaggtta
     4381 ataggatatt aaaatttgat tattctaata ataatacata tttaagaaaa aatcaactta
     4441 tttatataaa tattgctaaa atttaaatcc aaaatttgtc aattcttgtt ttaacacatt
     4501 aagattagtt tcttccattt cagctagagg aagtcttaaa tcaccaacat taaaacctaa
     4561 aatgttcatg gcagttttaa caggaactgg atttacttct ataaataaag cagcaattaa
     4621 agcatccata tcaagttgta atttttttga gccttcaata tcaccttcaa agaatttagt
     4681 tactaaatca tgtgtttctt taggacatat gttagcaagt acagagataa taccacttcc
     4741 acctaatgat aatagtggta gtatagtatc atcattaccg gagtatatag caaagtcttt
     4801 aggtactaat ttagctattt ctgcaacttg agctaagtct ccacttgctt ccttcacagc
     4861 aactatatta tctattttag caagttcagc cacaacagat ggctttatat tcactttagt
     4921 tcttcctggt acgttgtata aaataattgg caacttaacg ctatttgcta ttgtttcaaa
     4981 gtgaagttta agacctttct cattagtctt attatagtaa ggagttatta ttaaaagacc
     5041 atccacccct aatttttcag cttcttgact catatgaaca gacaacatag tattgtttga
     5101 accagtacct gctataacag gtattctctt attaactttt tcaactgtat attttattac
     5161 agcaaaaatt tcagattcac tcatagtagt cgcctcacct gttgttccac aaacaactag
     5221 tgcatctgtg ccattctcaa tgtgatattc gattaattca cctaattttt caaagttgac
     5281 attattatcc tctgtaaatg gggtaactaa agcaacagca gaacctttaa ataacatata
     5341 atcaaatctc cttttttatc taaatttaat tttttaagta caataataat tatagtacaa
     5401 attaattaat tttaataggt atagttaata tcttaactgt ttaaataaga tatttatatt
     5461 atattaatat tagaaaaatt ttaaataaat gttttattct caaaatatgc atattaattt
     5521 aataaaatat accaaaatgg aaataaaaaa taaattttat gagaatttaa taactaataa
     5581 ttaatatatt taggttataa taaatatgtg cgtaaaatct aacaataagc aataataccg
     5641 taaatttagg aggaataata tggatacttt aaaggaactt tttaaaatag gaagtggacc
     5701 ttcaagttct catactatgg gaccacaaag agcagcagag agatttaaga atgaaaaccc
     5761 agatgcagaa agttttagag caatattata tggtagtttg gcagctacag ggaaaggtca
     5821 cttaactgac tatataattg aaaaaactat agcacctaaa aaagtcgaaa ttgtttggga
     5881 agaagatatt ataaaagatt ttcacccaaa tgggatgaag tttgaagctt tagataaaga
     5941 taaaaatgta acagctgaat ggactgtata ctctgtaggt ggaggaacta tagcagaaga
     6001 aggtcaaaga aacagtaaaa gcaatagtat atatcactta gatactatgg atgagatagt
     6061 aaaatggtgc aaagaaaata ataagacttt ggtagatttt gtgttggaat gtgaaccaaa
     6121 agacataaaa gattatataa aaactataaa agatgctatg agaaaatcta ttgatgatgg
     6181 actaagtaca gatgaaataa taccaggcaa gttattatta aaaagaagag caagtacatt
     6241 ttataatgca tataaaaaag ataagagctt ctcaacactt gtatatgcat atgcactagc
     6301 agcttcagag caaaacgctt ctggtaatat aatagttaca gcaccaactt gtggttctgc
     6361 tggagtaata ccaggaatat tctttgcaat gcaggatttc tataattatg atgacgaaaa
     6421 aataattgaa gcactacttg tagctggaat aataggtaat ataataaaaa ctaacgcttc
     6481 aatatctgga gcagaggtag ggtgtcaagg tgaagttgga gccgcttgtt ctatggcagc
     6541 agcagcagta gcatacttaa aaggtggaac tatagaccat attgaatatg cggcagaaat
     6601 agcacttgaa catcatttag gaatgacttg tgaccctgta tatggatatg ttcaaattcc
     6661 atgtatagaa agaaatgcaa tggcagctca aagagcttat gatgcagcta attatgcatt
     6721 attaactgat ggctcacatt ctgtctcctt agaccaagtt gttgaaacaa tgaaagaaac
     6781 tggtatagat atgatggata agtacaaaga aacagctaag ggtggacttg ctaaacattt
     6841 cttctcttgc taaaaataaa attttccaat aataattcct ctgttttgat ttatgaaaca
     6901 ggggaattat tattaatggt gaattattta ataatgcaaa atatttttat attcaattta
     6961 gctttttatt tttaattagt ataacatgct atataatatt ttaattataa agaaatatag
     7021 aaatattttt taataaatta gaaattggag gagattacat gagagaggca tcatatttac
     7081 aagaacaact ttataaacat aggaagtcat taaatcctat tgcagagatt gggagaaaag
     7141 agtataagac ttcaaaatat ataagagatt atcttgataa attaggaatt gagtatgaag
     7201 tttatttaga aacaggtata gtaggtgtaa tagaaggtaa aagacctaaa aaagatattg
     7261 cttttagagc tgacatagat ggtcttcata gtgaagaagg ggtaaaacat ttatgtggtc
     7321 atgatggaca tgctactatc ttacttggat taattgagta tttaaatgat aataaagaag
     7381 acttaaatga taatatagta tttatattcc aaccggcaga agaaggtcct gggggagcaa
     7441 aagctttaat agatgaagga gtgcttagaa aatacaatat tgatgaaata tatgggcttc
     7501 atatatatcc agagttacca gaaggttatg taggtacaag agagggctat tttatggctc
     7561 aaataggaga tattgacata gaaatagtgt ctaaaagtgg acatggggct atgccacaaa
     7621 atggtattga tggtatagtg atagcttcaa actttgtaag ctcacttcaa actatagtgt
     7681 ctagaaatat aagcccaata gacaatgcag tgttgactat tggcagaatt gaagggggag
     7741 ctagaagaaa tataatagct gaaaatataa agatggaagg tactatgaga tgtttcagcc
     7801 cagaagtgta tgagaaaatg aagcttagag ttagagaact tgctagaggg tttgaattgg
     7861 cttataactg taaggttaat ataaatatta tagatgatta tatcgctgtt aaaaatgaca
     7921 aggatttgta tcaagagttt gtagaggcta ttggaagtga tactgttgta gagttagagc
     7981 cacttatgat atcagaagat ttttcttatt atcaaaaaga agttccagga ttatttttta
     8041 tgttgggttc aagaaacgaa gaaaaaggat ttgtaaatgg tcttcataat ataaacttta
     8101 attttgatga aaagatatgt gtaaatgcat tgaatgttta ttcaaaatta ttaaaataca
     8161 aagaagcaat agattaggaa gataagttat aaagattaaa aagcataaat acctaaattt
     8221 aaaaaaagtg tattaataaa taccccttac atatttgtat aaatttattt taattgacaa
     8281 aaatacaaat aagaaggggt gatttttttg aaagaaaaag tacaaagaga aaagaagata
     8341 actaatggca agaatactaa aatattgaca aatgatgata taatgaaata tgagatagcc
     8401 tcagaactag gtcttatgga taaagttggt gaacgtggat gggcaggact tacagctaag
     8461 gaagcaggaa gaattggagg tatgcttaca tcacgcaaaa agaggcttaa aaagattgtg
     8521 gaggagcaga ataaaaagga tgagtcaata tagtgatttt gcgtttgtat atgatgaact
     8581 tatgaatgaa gttgattata atggttgggt taaatatata gaagatataa tagatagtga
     8641 aggtgtaaaa gtaaaaaata tattggaatt ggcatgtggc acgggtaatc ttacaattcc
     8701 attgactaaa aaaaattacg atatagctgg tatagatata tctgatgaga tgcttagtgt
     8761 agcaagagaa aagtcagaaa aagaaggagt agaattagtt ttacttcaac aagatatatc
     8821 agagcttgat tttgaaatat ctgatttgga ctgtgtactt tgtgcttgtg atggattcaa
     8881 ttatataact tatgatgatg atttggaaaa tgtattttca aagacatatg aattattaaa
     8941 gaaagatgga atattcatat ttgatataag ttctttttat aaattagcca acatattggg
     9001 aaataacatg tatggagaaa atagagaaga tatagcgtat atgtggcaaa attactttga
     9061 tgaagaagag aatctaattg aaatggaact tacattcttt attaaagatg aatgtggaaa
     9121 atttgaaaga tttgaagaga tacaccaaca aagagcatat actgaagatg agatagttga
     9181 tatgcttgaa aaatctggat ttaaggatat aaaatattat ggggatttta catttgacaa
     9241 acctagtgaa gaaagtcaaa gaatcttctt tgtatgtaaa aaataagata tttttgacta
     9301 tataagataa ttattaaaaa taaccatgat ataaaaatat agaaaatgca gttttataga
     9361 ttaaaagtaa agtgatgata ctttatgact tttatttttt aataattata aagttctata
     9421 gataaaaatt aaaatataat aactattgct tagtaaaatt aaaataaaat gggttttgcc
     9481 caaggttaag atagataagt atttatagat taaaattaag gtagtaatat aataaaaata
     9541 agacaattaa cgggacatgg aggaatatta atgagagatt atgtactaag agcgacatca
     9601 ggaaatggtc aagttagagc atttgtcgca actactagaa atacagtaga agaagctaga
     9661 agattacatg aaactacaaa agtagcaaca gcagcacttg gaagaacatt aacagccaca
     9721 tctataatgg gattaatgat gaaaaatgat agtgataaat tgactgtaat aataaaaggt
     9781 ggaggtccaa ttggaactat tatcgccaca tcagactcta agggaatggt aaaaggatat
     9841 gtaggaaatc cacaagtaga ggttgaagac tatccaaatg gaaagttaaa tgtagcagca
     9901 gcagtaggta cagaaggtgt tgtaaaagtt ataaaagact taggtcttag agaaccatat
     9961 aatgggactt atccattggt aagtggagaa attgcagaag actttactta ttattttgct
    10021 gtatcagagc agactccatc agtagtagct ttaggtgttc ttacaaaaga agatgaagta
    10081 gagtttgctg gaggatttat agttcaatta atgccagatg cagaagaaga gactattgct
    10141 aagttagagg aaaatgtagc taaattacct tctataacta acatgttaaa agaaggaaaa
    10201 tcaccagagg atatattaaa tattgttttg gatggtttgg aacctaaaat acttgatacc
    10261 tgtgaagttg ggtttatgtg tgaatgttca aaagaaagag taaaaactgc tttggttgca
    10321 attggtaaaa aatcattggc tcaaataata gaagaagata aaaaagcaga agttggttgc
    10381 cagttctgta ataagaaata tatgtatagt gaagaagagt tgcttgaaat tttaaaggaa
    10441 atgtagacta taaaaatagt tgtattgata taagtaatgt tatagtgata tagtaaagtc
    10501 gtattgatat agtaattaaa gtataattta ttaataatag acatatttat attaaaaaat
    10561 aagtttaata aatttttaag cttaataaat tttaagaatt aagaagcttt ctggctgtaa
    10621 tgtttgcaat gttgcagtta gaaggctttt ttattttatt atagttcgtg tcaaagattg
    10681 tatgaaacaa taaaatattg taaaataatt tgtatattta gacctgattg gcacaaaggt
    10741 ttataaggta tttgagttga ttatgcgaaa ttctatgaag tagtaaccct gtgactttaa
    10801 tcatgggagg ttcagaagaa ggagaaaatt ttaaatatgg aaagtaaaac gaagtacata
    10861 ggaataagta tattaatatt attgatgata tttttaattg caaaaaattt taacggaaaa
    10921 cttgaaatac ctattcctga gagtaatagt agaaaattta aatcagcagc agtgttttat
    10981 cctcaacatc aagatgatga ggttttatgg ggaggaagtg ccattgtaga tgctataaaa
    11041 cagtgtggtg ttgataatgt atatgtggta ttagtatcag atggttctgg agttaacgtt
    11101 ttcaaagcta acacaaaatt tagaaattta actaggaagc aaaaggaaga acttagaaat
    11161 aacgagttta aatcagcttt gagagagctt ggagtgaaac ctcaaaatgt gattatactt
    11221 gcagatatag ataaaaagga aggcacacat tatgaattaa tggaaaagac tatattggat
    11281 tttgaacata agtttaaagg caatgtgacc catatagccc atcactataa gtatgatgac
    11341 catataatgc atagaaagaa tggggaagtt ttaaagaaac ttaaacgtga aggtaagata
    11401 aaagatgact tgtattttgt taagccaaaa tatgtaaagt atataccaag aaatgaaaga
    11461 gctatctata aagcaatgga ttttgatgac tacaataagg ttaagagtgc ttgttatgaa
    11521 tataaggttg taaattcaaa gcgtaaaatg tatggtatag gatatatttc ggcacatagt
    11581 tattttgaac atcttctaaa tgaccctgaa cttacgtctg tattaagtga agattaattt
    11641 cttataagta gtaagttatg tttgataagt tgattggaat tttctatagt tttgaaggta
    11701 ttggaaattt agttttattt aaagatttaa agatagaggt ttttagtgtt taaaatttta
    11761 aagttaagtt gaataattgt atagaattat ttgtgaaaga agaaacagtg taaaacatag
    11821 tatcttattt atggttttaa aatcataggc aagatacttt ttttatgtaa ctaataccat
    11881 tttgagatta ttaacttaag aattttatat aatttaacac tcatatataa tgatgatatt
    11941 taggtagaat agatgcaatt acgcttaaat aaaataactg tattttttat aaaaatcaaa
    12001 ccctacacta tactaccaga taaaatctac atgctataat ttgacctgtg tttataaaaa
    12061 aaggagtaat ctaataaaat agtatttcac ttatgtataa ctaacaacta ttattacgat
    12121 tttctctaaa taatttaaag aaaaataatg caggaggtaa aatatgaaca atgaatttat
    12181 aattttaaaa ggttgcaaag agaacaattt gaacaatatt tccttaaaaa taccaaaaag
    12241 aaaaataacc atattcacag gggtgtcggg ttctggaaag tcatctattg tatttgagac
    12301 aattgcaaaa gaatcacaac gccaactaaa tgaaaggttt agtacatttg taagaagttt
    12361 tttacctaag tatggagagg ttaaagctga ttgtatagaa aatttgtcaa caccaattat
    12421 catagaccaa agtagacttg gtggaaattc aaggtcaacc ctaggtacta ttacagatgt
    12481 aaattccttt ttaagagctt tatattcaag gtttggtagt gagtatatag gtaatgctaa
    12541 tatgttttct tttaataata ttgatggtat gtgcccacac tgtcatgggc ttggaaaaaa
    12601 gctagttcct aatatgaacc aaattcttga tatgaataaa tcattaaatg aaggtgctat
    12661 attgctttca gggtttggag tgggttcttg gcattggaat atattcgctg aatcaggatt
    12721 ttttgatatt aataagagga tatgtgatta ttcagaagta gaattggaaa agtttttgca
    12781 tggtaaagca gaaaagatta agatagaaaa tgctggtcaa actaacatga catatgaggg
    12841 attaatggtt aaatttaatc gcctatactt aagtagagag ggagaaatat ctgaagctac
    12901 taagaaaaaa ttgagtaaac ttcttataga agatagatgt cctatatgtg atggaagaag
    12961 acttaatgaa agagtatata ggtctttaat taatggttat aatatagcag atttaactag
    13021 tatgcaaatt gatgaattgg ctgaagttat taaaagtata gatgagccag aagcacaacc
    13081 tttaattaaa ggaataatag aaaagttaaa cagtatcata gatataggtc ttggatattt
    13141 aacactggat agagagacat cttctctatc aggaggggaa tctcagcgta taaagatggt
    13201 taagtattta aatagtaatc ttgttgattt aatgtatatt tttgatgaac caagtgtagg
    13261 acttcatcca aaagatgtgt ataaattaaa taatctttta aaaaaattgc gtgataaagg
    13321 gaatacaatt atagttgttg aacatgaccc agatgttatt aagattgcag accatattgt
    13381 agatgtagga ccaaaggcag gtaagtatgg tggagaagtt gtgtatgaag gcagttatga
    13441 aaatctactt acaagtggta ctttgacagg gaatgcttta agtaaatttc ttcctattaa
    13501 agaaagtgta agagaacata gtggatattt agaagttaaa aattgtaata aaaacaattt
    13561 aaaaaatgta tcgattaaga tacctaaagg tgtattgact ctagttactg gagtagcagg
    13621 agcaggtaaa agtacgttga ttaaggatga atttttaaag caaaatccta gtgctgtact
    13681 tatagaccaa agccctgttt cagcaaattc aaggtcgagt ttggcaacat atagtggaat
    13741 tatgaacaac ataagaagca tattttcaaa aacaaatggc gtaaatgctt ctttattcag
    13801 ttcaaattca gaaggagctt gtgataactg taagggttct ggaatagtag aaatgaatct
    13861 tgcttttatg gagagtataa agagtacctg caatgtttgt gaaggtaaaa aatttaaaaa
    13921 agaagtttta gaatataggt ttcaagggaa gaatataatt gaagttcttg aaatgtctgt
    13981 tttagaggca attgagtttt ttaatttaaa acagattaaa acgaaattac aatgtattga
    14041 aaaaatgggt attggatacc ttactttagg acaaactctt gatactttgt ctggaggaga
    14101 gtgtcaaaga ttaaagcttg ctagtgaatt aaataaggaa agttcagtgt atatattaga
    14161 tgaaccaaca acaggtcttc atatggctga tattgagaat tttataagta taattgaaga
    14221 gattgtagat aatggaaata cggtcataat aattgaacac aatatagatg taattaaaag
    14281 agctgattgg attattgagc tgggacctga aggaggaaca aaaggcggga gagtaatatt
    14341 tgaaggtatt ccaaaacaat tatgtaacaa taaaatatca ttaacagcaa aatatatagt
    14401 ataataaata aaatataata aaattataat gatattttaa taaatataat gggtatataa
    14461 aatgataatt aaacttaaca gtaataaata atgtgagaaa tattcaaatt taattaaata
    14521 aagtgtcaga aatttgttta aattttagta ttttaacggt tctgaccttt tttcttattt
    14581 ttatggagaa tatcgaggat ggagtctcta atagttactt ttttgtgtct acttgcaagt
    14641 ttgattaaaa aatggctata cacacttgtg acttcagtca tgggaggttc agattataat
    14701 cttaatatat aatttgtgaa ataatttgat gacgatttgc tgatgtataa acaataaaga
    14761 ttaactacat taaaatttaa atcttgttaa gtaaaactta taaaaggaga gagatggatg
    14821 ataaatgata ttctaacctt attatctgaa agaagtgatt tttttataaa gctatccata
    14881 gaacatttga tgatttctat tatttctata ataattgcga ttgttttggg tggtataatt
    14941 ggtattattg taagtgaatt tcataattct tctaaaccta tattgggaac tatcaatttt
    15001 ctatacacca ttccatctat ttctatgcta ggatttctga taccattttc cggaattgga
    15061 aatactactg ctgttattgc acttaccatt tatgcattat tgccaatggt tagaaataca
    15121 tatactggaa ttactaatgt ggataaaaat attcttgaag cagctagggg aatgggaagt
    15181 acagattttc agatattgta taaaataaaa cttccgttag ccttacctgt tattatgtct
    15241 ggtattagaa atatggtgat tatgacaatt gcattagctg gtatcgcttc atttataggt
    15301 gctggaggtc taggtgtagc catatataga ggaatcacga caaataacac aataatgaca
    15361 attactggaa gtatacttat tgctctatta gctttaatat ttgattttat tttagggctg
    15421 gtagaaaaat tttttcaacg aaaaagaaaa aagaaaaata ggaaaaaagt ttttgccatt
    15481 ctaggaagcc ttattcttat agcaactatc tttacgtctt atttatttaa tccttcaaag
    15541 actcaaacta ttcatattgc tactaaacct atgactgaac aatatattct tggtgagatg
    15601 ttggatattt taattgaaaa aaacacaggt ctaaacgtgg aaattacaca aggtgtagga
    15661 gggggaacat ctaatatcca gccagctatg gagaatggag aatttgacat ttatccagag
    15721 tatacaggta caggctggaa tatggtactt aagaaaaatg aattatatac tgaaagtatg
    15781 tttagtaaac tacagtcgga atacaaaact aaacttaaaa tgcaatggac tggtatgtat
    15841 ggtttttcta atagctatgc attagcagtt agaaaagaag ttgcagataa atacaattta
    15901 aaaaatataa cggacttaaa agatgtatct tctaaattga catttggagc tgaatatgac
    15961 ttttatgaga gagaggatgg atatactcca ttgtgtaata catatggact gaactttaaa
    16021 gatacagttg atttagatat tggattaaaa taccaagcta tcaatgaagg taagattgac
    16081 gtaatgccta tatttacaac tgatggtcag tatagtgttt cagatgttgt agttctaaaa
    16141 gatgataaaa atttctaccc atcttatcaa tgtggaaatg ttgttcgttt ggaagtatta
    16201 gaggaaaatc cagaactatc aaaggtattt aagatggttg gagatatttt gactgataaa
    16261 gaaatggcta aaatgaatta tgacgtagaa aacaacaatt tagaacctag agaagttgct
    16321 cgtgagtttc tgaataagaa aggtctttta taaattggag gaaatataat gacacctatt
    16381 atacaattta aaaatattaa aaaacaatat aatgataaaa ctataataga taatcttaat
    16441 ttagatatag aaaaaggaga gtttttgact gtaattggtt cgtctggttc tggtaagaca
    16501 acactcttaa aaatgattaa tggtctgatt ttgccagatg gaggaaatat tttgataaac
    16561 caaacagata tcaaaaacga ggacttaatt aaattaagaa gaagaatagg atattgtgta
    16621 cagggaagtg ttctttttcc tcatatgact gttgaagaaa atatttcata tgtaccaaat
    16681 ctcctaagta aaaagaataa attagaagta aaaagtgctg taaacaagtg gatggaaata
    16741 gtgggattgc caaatgatat gaaagtgcgt tacccatcag aattgtcagg tggtcagcaa
    16801 caacgtgtag ggattgcaag agctttagct tcttctccag aaattttatt aatggatgaa
    16861 ccttttggtg cagttgatga gattacacga aagcaattac aaaaagagat aaaagaaatt
    16921 cataaaaaaa caggagttac aattatcttt attacgcatg atatttatga agctttgatt
    16981 ttgggaacaa agactttggt attaaatcat ggagttgttc aacagtatga tactcctgag
    17041 aatattttaa atactcctgc taaccaattt gtagaccagt tattgaatat aagaaaatct
    17101 ataatagatg atgataattt aaaagaataa atttaaattt aaaatcaaat tgttgtgata
    17161 aaaactattg atttagagct aacttcaagt tgtatacttg aagtggaaaa gttagattaa
    17221 ggtatagaag ggcatttagc agttggctgt agacgaggta actgctgaag gtgctttcaa
    17281 taaatggcta gaacctgtat ctgttgaaga ttataataaa ttgaaataag aagctcaata
    17341 tacagagtag ataggcactt caaatagaag tgtcttttgt gtgccataaa ataaatcaaa
    17401 atattagcaa agaatgtgaa aaacaaaata aaataatatg ctatctttaa aataggaggg
    17461 atgctatatg gataacaatt tgattaccat tgaaaaaact ttagattata tagaaaaaca
    17521 tcttttagat gagatgttag atttaaatgt aatttctaaa gagataggct attcaaaata
    17581 ccatttacat agaatgttta catcagttgt cggatttagt gtacataatt atatacaaag
    17641 acgtagactt actgaagtag cgagaatgct aatttttaca gaaaaaccaa ttattgagat
    17701 tgctttaatc tcggggtatg atacacaacg ctctttttca agagggttta aaaaaatgtt
    17761 taaacgtagt cctaattttt ttagaagaca tcaagatttt ttaccactac aattaaaatt
    17821 tgatattcat aatagaaaaa aattacgtgg agatatggtt ttagatgtta aaaaagttaa
    17881 ttgtgcagat ataaatattg ttggctattc tggtaaagta agaaaaggat ttcatattat
    17941 tggaaaatgc tggagggatt tgcataaaaa taaagagatg atagaaaata aggtagattt
    18001 aggtttttta attggagtta gtgattatag caattttaaa gagattgaga atactcctac
    18061 atttacatac attgcaggtg ctcaagtagc tacttttggc aaattaccta atggaatgga
    18121 aaaattcgta ttaccgaaaa gtaattatat tgtttttact tttattggga aaaatgagga
    18181 tagtatgcaa aatgttgtag aatatatcta tcaagaatgg ttccctaatt caagttgcca
    18241 gtttaatgaa aataatttat atgactttgt aaaatatgga gaaaaaactg atgagaatgg
    18301 tttgagtgaa attcagtttt gggttccaat catttaaaag aaggtattat gtataaagta
    18361 gatggattta tataggcaaa gttttacagc agtatgtatt tataaataat ttatagtttg
    18421 taggaaagaa ataattttac tataagatta tgataaaata cacaatacta tgacaaaata
    18481 gtgttaaata atagatttaa ttttgcatag gaaaagttct ataatggtgt gtattcataa
    18541 ataatttata atctgtagga aagaaatagt tttactataa gattatgata aaatacacaa
    18601 tactatgata aaatagtact aaataataga tttaattttg caaaggataa ggtgttttaa
    18661 taaaaatgaa tgtagaaaac gaattactta aaaacctaga tagattgcat acaacagagt
    18721 taggcgttgt aagaattaag aaaaatcttt ctttggagac aaatgatgta gtgaactggt
    18781 gtaaaactaa aatagaatct ccaaatgcca ttattaatag aaagggaaaa aactggtata
    18841 taagtgtata tgattgtatt ataacagtaa acgctcacag ttatacaatt attacagctc
    18901 ataaagaaaa gaaatgaatt gcaaaataca aaggtcaaaa gcattttcca ctgtttgtag
    18961 tacctatgaa gaagtatata aaataatatt acttttgaat aataatttaa attttggagg
    19021 ttgaaaaaat gaatgatact gtaaaagaaa aattggcaga gtcactaact tctgatacta
    19081 ctgatatttt accttatttg ccttatttat tacaagattt ttgggaatta ggaagttcac
    19141 cagaacaaat gttacaatta tttagaaagc atataccttt taatgaggat acaaaagttt
    19201 tggatttagc ttgtggaaaa ggtgctgtat ctgtgaagct tgcaaaagag ttgaatatac
    19261 atatcaaagg tattgatttg atacctgaat ttattgatta tgctagaaga aaggctattg
    19321 aatataaggt tgatgctttg tgtgattttg aaatgaatga tgctaatagt tctgtaaaaa
    19381 ttgagcatga ttatgattgt gtgatttttg gagctgtagg ggatatttta ggtaattata
    19441 aagaaactat aagtaagcta ttacctacaa taaaaagtaa ggggttcatt ttaattgatg
    19501 atgcatatgt agtagataaa tctacaaatg aacagcttaa ttttgagagg gtgtatccca
    19561 cttacaatga gtggtgtata ttgttcaaag aattaggtct gaagctaatt gaatgtatac
    19621 catgtttaga acttgaaaat gatgttgaag aggaaatgaa ttggattata actcgtgcaa
    19681 atgagctaat taaaaaatat ccttctcaaa aagatatgtt tgaacaatat gttattagcc
    19741 aaaaaaatga atatactgat ttacagcagg accttgttgg agcaacttgg attttacaaa
    19801 aagaataaga taaatattag actagataat caatgttata gtgaattatc ccatgtctag
    19861 tgaatataaa taacataaaa aaccgtcaag atgaatagtt tattcgtgct tgacggtttt
    19921 tagtatttgt tgaagtaaaa ttttatttct ttagtatatc acttttagtc aatccatttg
    19981 atttagcttg agtattccat tgatgattcc ccataagttt atataggaat ctagggaagt
    20041 taggtgaaat aaaaatagta cttttgttgg aatcatctat atgtatatta tcactaagta
    20101 tattttttga aagttctttt aatgcaattc ctaaatcttt tttaggtcct ttacccaaag
    20161 gtacggattc tagtgaacca aacatctccc cagctccaat tccaatacct tgaacccatg
    20221 taaagttgat ttttttacac caatgctcta tcatttgaat tgccaaatga ttatgttcac
    20281 cttcattgaa tccacagtta actattgcat acactggaat aggtttttta ttttgtgttt
    20341 ttaaaaattt ttcacatttc tctaaaaaat gaagtaaatg agatggaata ccatctacat
    20401 aaagaggaga tgcgattatt aatctatcac aattaagtat ggcattaaga gcgtcaggat
    20461 acttagatgt gttatctact ttaaatgtaa aaactttatt atcattttga ataagttgtt
    20521 ttaattcaga taaaaaatac tgtgaattat ttttatttac ttttggacta ccatttatca
    20581 ttactatatt catatggatt cacctcttat atcttgtata ttttcataga aacaaacaga
    20641 gtgttctttt atattgaaat ttatagcatt tccatttact aatttcgtag cagtttcttt
    20701 ttcagcttct gtaatacaat ctccatagaa tatagctttt aaatttatct gtttatcata
    20761 tctgctttta tgacgcatca tattatcttt tattacaaag tatggaagaa tataattaat
    20821 gcttttatca agtacgtttt tgacaaaagg gctaaaccct ccgtaacagc atctgcttat
    20881 tataataatt ccatcagagt catttataag tttacccatg ttgctgtaat aatcttttac
    20941 aacacattca gaaggagttt taatccagca accaaaacaa ccaatacatt tgtggatttc
    21001 ttttgtgttt ggaagtattt ttatattgtc atcaagtttg ggtaataact tttctaattc
    21061 tttgtcagtt aaatcatgta ttaaaagcct cataaagtcc atctcctttt attatgtatg
    21121 tttacttcta aaaattatat cttatagatt tttcagtaaa tctttacacc aattaataac
    21181 aaaattataa tagtcaactc cataaatcat tgttgcatac atatatgtat attggtcaac
    21241 acaatgattt tcttctgcat catttttttg tttacttaat ttatttagaa gtaattctac
    21301 ttcagttatt aacattttta agtttgtttc aattatttct ttgggtagat acttgtaaaa
    21361 gaatatatta acaaggtggt cttgctttgt tttagaaaaa tttataggtt tctttaacca
    21421 ttcaagaaat acttctttac cagtattagt tatggaatat agttttttta atttgttacc
    21481 atctataact tcgtgatagt gtatatatcc ttttgtttct aatcttttaa gtgcaggata
    21541 gatacttcca aagctggcgt caaaaaagta cgaggtactt tctgacatta tttgttttaa
    21601 gtcgtatcca cttaattctt tttccattaa aaatcctaaa ataatgtatt ctaacatatt
    21661 acctcctggt atcaattaga tatatatcga ttcgatatat ttattattac agataataaa
    21721 tcttacgtca agatgctttt taagataagc taaaaagaat taggagttat tgataaatat
    21781 gcaaaaaaat taatataaaa ggtaattaaa tatcaataaa gatttaaaaa tagttaaatc
    21841 aaagtaagag tctaatatat aaaatttata gtaactttat ggtttaaata taaacttgtg
    21901 tgctattgac aaagtataaa attagcagta gaataattgc atgagcaaat agattgttgc
    21961 gaacgcaaaa aaaggacaat agatattaaa ggagagaata tgagagagct attaaaatgg
    22021 gtttccatta taaatagata caatacaaat tatattgaca ggaaattggc ggatattgga
    22081 atcaataata gccaatattt ttatgtatta cacatatgta ataatccagg tattacacag
    22141 gacactatat ttgagaaaat tcttgtgaat cctagtaata taacaagagc tttggcaaat
    22201 ttagaaaaag aaggctttat aacaagagaa ccaggtgtaa aggataaaag aacatggcat
    22261 ttatatccta cagaaaaatc tttaggatgt tatgatgaaa tactaaaaat aacaaatggg
    22321 tatgttgagg agttgttgag ttcatttgag aaagaagagc aagagttgtt tatgtcaatg
    22381 ttaaagaaag ttgcattttt ggctattgat atgaatgaag aagcaaaatt ggaggcgaag
    22441 gaaaatggaa gaaaaatata aggagccatt ggaatatgaa aaggtttcaa agttattaac
    22501 taagtacgca attcctagta ttatagcaat gttagtaagt gctttatata acattgttga
    22561 ccaaatattt attgggcaag gtgttggtgt attgggcaat gctgctacaa atgtcacttt
    22621 tccactaaca acaatatgta ctgcaattgc attattgtta ggaattggag gagcgtctaa
    22681 gtgttcattg gaattaggtg ctaaaaattc agaaaaagct acaaaagcag caggaaattc
    22741 tatatgcttg atggctgttt ttggaataag tttatttata gtagtatcaa tatttttaac
    22801 accactatta aaattctttg gctctacatc agaaatacta ccttatgcac acacatatac
    22861 aaaaattaca tcaattggat taccatttct tataatgtcc actggaatga gtaagttgat
    22921 acttgcagat gggagaccga aagcttctat gttttgtatg ttaataggag cagttattaa
    22981 tacaattttg aatccattat tcatttttgt ttttaatatg ggaattgctg ggtctgcttt
    23041 agcaactgtt attgggcaac ttatatcttt tggaataagt gttcattata taaagaattt
    23101 tcaacatatt aaattacata agaaaagttt taaattagat agtataattt ataaacatat
    23161 atttgcgcta ggttcaagct catgttttaa tcaattagca atgactgtaa cacagatagt
    23221 tatgaataat acattggcat attatggggc taggtcagtt tatggaagtg agattccact
    23281 tgcttgtgtc ggtatcatta ttaaagtaaa tatgattttt atatctataa ttattggtat
    23341 atcacaaggt atgcaaccaa ttatagggta taattatggg gctgaaaagt ataatcgtgt
    23401 aaaagaagca tataagtttg cagtgggaag tgcaactatt atatctgtta ttgcattctt
    23461 atgtttccaa ttattcccaa gacaaattac gtctatattt ggtaacggaa atgagactta
    23521 ttttatattt gcagaacatt attttagaac tttcttgttc ttaacattta taaatggtgt
    23581 tcaaatagta tcatcaactt tctttacatc aattgggaaa tcagtgttgg gactttttac
    23641 ttctttgact cgtcaaattt tatttttaat tccattgata gtaattttac caatgtttat
    23701 gggaattgat ggagtaatgt ttgcaggacc aatagcagat gcagcagcgg caatggtatg
    23761 tatatacttt gcagttcatg aaatgaaatc attgactata aagcaaaata atttgatgag
    23821 taaccataat aagattgcag aggtataaga caatatttaa gaagtataga aaattaattt
    23881 aaagaagtat aataaactaa tttaggaagt atagaaaatt aatctaaaga agtataataa
    23941 actaatttag aaagtataga aaattaatct aaagaagtgt aataaactaa tttaggaagt
    24001 atagaaaatt aatctaaaga agtgtaataa actaatttag gaagtataga aaattaattt
    24061 aaagaagtat aataaactaa tttaggaagt atagaaaatt aatttaaaga agtataataa
    24121 actaattcag gaagtataga aagctaatct aaagaagtat agtaaactaa tttaggaagt
    24181 atagaaaatt aatctaaaga agtataataa actaatttaa gaaatataga aaattaattt
    24241 aaagaaatac aatacattaa tttttaagta acattaaatt aatgtaagta aaataatgtt
    24301 catatgaaga tttaaaggtg cattttctga agctaagtat atgttgtaga gtgttaggtc
    24361 tctctatcct gtgaagttta atagctcaag aaaatgcact atttatgatg attattttgt
    24421 tttatttcca attttggaaa taactattct aattaaatct aaaattatac cataaataag
    24481 aaaagaaagt atagatgcta tgtaccaata aatcgaaatt gaccctgtaa ttgttctaca
    24541 tgggatttgc atacctgaat ccataaaaac aaaaaaacta tcaactctgg aaataataga
    24601 caaaatagga tttaaaccta ttagaacaat actttttgag tcttgaccaa tttgatgcat
    24661 ataaatctca aataatgcaa ttaaaaaaaa ccaaaatgta aaagatttaa taattttttt
    24721 catcaaaata cctcacataa tataaattct gtttgcacta tttgcttact acaactattt
    24781 atctccattc agtcacatca cttaattgta atctaggtct tcttgatggt tcttcatcag
    24841 gataacccaa agatattgct gccactaatt gactatctgt atttaaccat tcacataatt
    24901 cacgatatgc aaagtataca tcacaaatcc aaagactacc tagacctaat tccacagctg
    24961 taagcgacat attctgaatt gcagctccaa ttgattgcat gtttgccatt tcatagaatc
    25021 gttcttcagg ggatagcttt tctaaaggtg atttacctaa tatatttaaa ataaaaattg
    25081 ttactggagc ttgtttcatg atttcaactg tgtaatttgc accagcgata tgttgacgac
    25141 ttcctggaag aagcccatta tcattaattt cattttgaat acctttgctc atggctttaa
    25201 gcatagattc tttttccttt tcagtgatga caacaaaacg ccatggttgc ctgtttttag
    25261 atgaaggtgc atttattcca gcttcaatta ttttctctat agtttcatgt gaaatgcttt
    25321 gatttttata ttttctgata cttcgtcttt ttgatataga atcacttatc attttaagta
    25381 acctccgttt attttttgga aaaatctatt taatttttat tttctatttt aattatttta
    25441 gcattaatca gaaaaaaagt cattgatttt agtatacaat tattaatgat tatattaaga
    25501 attgtatcaa aaagtatgtg attatactaa gggattatat taaacattat atttaaaatt
    25561 gtatgaaagg ttgtatttaa tattatattg agagatatat atattaggat ttatatatat
    25621 tcagatttat tattatattg agccttatat taaattaagg actataattt gaaaataagc
    25681 tataaaaact taatattgtt ttgttcagag ataaaaaaag aatactttag gtgattttta
    25741 ttaaaaatta agagtgataa aattatatac tttataaatg taaatatgac gaataaaaat
    25801 gataaatata ataatatata tatttacatt aggagttttc tatactataa ttatttaaaa
    25861 ctaaatataa atgttggaat tatttaggac ttgaaattgt ctaaaaataa atacaagtga
    25921 tgaagaggtg atgtgatgag attagatatg gatagtatgt tatatgtact aatttatatg
    25981 acattattgg tatctatcat tggaagtata ttttattttt gtaaagacct cttaaatcat
    26041 aaaattaagt atgttttaat aagagacata tttgtaaata gaattacata ttctgaagaa
    26101 cgactgccta aacagtatat agtttttcag aaatatgata tttggaggta ttgtagtttg
    26161 ttttaatttg aaatgaatct attataaatt ctaaagttta aaaataaaaa ggagagaata
    26221 aaatggattt catttcaagt atattgcaag gattattaag tatattattt aattttacag
    26281 gtgattttgg aatttctatt gtacttataa ctttaatagt taaattagta cttatgccac
    26341 tatcattaaa acaaagattt tctgtaaaaa aacagcaaga attagctgaa aaaatggaat
    26401 atataaaaga aaagtataag aatgatacta aagaattgga aaagcaaata caagtaagtt
    26461 ctgtagaaag tatgaagagt atgctgggat gtcttatagt tttggttcaa atgccagtag
    26521 tatatgcttt atatcatgta tgtttagata tgacaaagga atttacaaca attataattc
    26581 catggattgc taatttaggt acatttgata atttgtttat attaccttgt atatatacat
    26641 taattatgtt agcttcaaat ttagtgaatt atataccata tttaaaaata aattctcaag
    26701 taaagtttaa caaacaaatg gctgtggtga ctaccataac aagtcttata ctaactgtta
    26761 gaactccagt agccataggt ctgtacttta taacaagtgc aatttattca ttaattgaag
    26821 atatttgctt tagaatatat tttaatcgaa aaaatagaat agtgacaaaa taatataatt
    26881 attattagaa atattttagc ctgctaacat agaaaaagtt agtaggctat ttttatctat
    26941 gatatcaatt ccattaagaa tagtctaaaa aatactatca aaatgctata aaaaatacta
    27001 taaaaaaata gttgaaaaat aaataaatta atataaacta aaataaagcc tacaaaaaag
    27061 aggtgataaa tagaaatggc taaaatacta gttgtagaag atgaaaaaag aatgcaaaat
    27121 attattgtag aatatatgca aaagggagga tatacttgta taactgctga tgatggagtc
    27181 gaggcactta caatattaaa gagcaacaat atagatttaa tgatattgga tattatgatg
    27241 ccgtatttag atggattttc agtgtgtaga gtttcacgtg aaatgactaa tatacctatt
    27301 attatattga cagctaaagg tgaagaggag gataaattaa agggttatga atatggcgca
    27361 gatgattata taacaaaacc atttagccca aaagtacttt tagcaaaagt taatgctttg
    27421 ttacgtagat acactactga tattcccaaa aattcattga gtttaggaaa gatttttatt
    27481 atggtagcat caagacaagt gtatgtagaa gataaattga ttgatttaac atataaagaa
    27541 tttgaattat tgcgtttatt tatggaaaac cctaatcaag ttttttccag agagaaactt
    27601 ttaaattgta tatggggata tgattttgaa ggaaacacac gaactgtaga tacacatata
    27661 aaaaccttac gcaaaaagct tggtagtgaa ggacaccata tagttacatt aattcgttca
    27721 ggatataagt ttgaggttaa ggaatgaata gtttaagaaa gattatagaa aaactgaaaa
    27781 atgtagatat ttcaaattgg attaaacaaa attttggact atattcagtt agaaaaaaag
    27841 tgtttttact ttctaaatta tcaggagcgt taattatatt attttatctg gttgttgaag
    27901 aattttcgat aaactctaaa tttgaatttt ggatttggtt tataatactg gttatattta
    27961 ttatgagtat tgatttctta ttgggaaaat ttatttcaga accaatcaca tctataaata
    28021 aatctgcaaa aagtatgtcc caacttgatt tttctaatcc atgtactgta aatactaatg
    28081 atgaatttgg agaactttct aggagcctga atacaatgtc tacaaatcta caacaggcat
    28141 tatcagacct agaaagtgca aatattcaac ttgaaaagga tgtaaataag gaaagaatgc
    28201 tattggaaca aagaaaggag ttggtagata caatttcaca tgaaatgaag actccacttg
    28261 gcattatacg tgcgtataca gaggggctta tagatgaagt tgatgaagaa aaaaagaaaa
    28321 attatatgaa tgttataata gaagaaactg atagaatgaa caatatgatt gtatcattat
    28381 tagacttatc tgcattggaa gcaggagttt ctaaacttaa cccagagcgt tttgatttta
    28441 tagagtttgt tgaaactgtg gcaggtcgtc ttttaattga tataccagat gccaattttt
    28501 attttacata tgatttgcca gagtatgaag tttttgtagt ggcagataaa atgagaatgg
    28561 aacaagtagt agagaatcta gttattaatg caaagaagca tgttgtatat aatggtaatt
    28621 tagatttatc gattacatgt gaaaatggct tgcttttatt tgaaatttat aatgatggta
    28681 ggtttataga gcaagatgag atttcaaaga tttggtctaa gttttatcgt agtgttcaat
    28741 ctcagcgtac aggaggttca ggtctagggc ttgcaattgt ttcacaaata cttacaatgc
    28801 aagggttaga atatggagtg aataatcgtg atagaggagt agagttttat tttatgattc
    28861 caattgatga ataatttagt attaatttat aaattataag tatctgatgt aaaatatttt
    28921 tatttgtata cgtaagtatt taaagagaat aaaataaaaa aacttaatga aaaagcttaa
    28981 tgaaaaagtc taataaaaag cttaatgaaa agcttaataa aaagtcttgt tttcctgatg
    29041 aaaacaagat tttttattaa gctttttatt atttcacttc gatttcacaa gagagacact
    29101 ctgatttcac caaatagaaa taaaataggt taaataaatt aaaaaataga agcgtaatta
    29161 aataaggtta caagggggag tacttatgaa atggtataca tggttgacta ttatattgat
    29221 aatattgctt tctattccgc ttaaaattaa atttataaaa tggtgggata gacgacaaaa
    29281 aaatgataaa ggaggttatg atagatgata aaagtaagag agttaaaatt ttcttatgga
    29341 aaagataaac agatattgca tggacttaat tttgaagtaa aagaaggaga aatctttgga
    29401 ttccttggac ctaatggttc aggaaaatcc acaacacaga aaatcttaaa tggtgtttta
    29461 aaaggttaca atggtcacgt atccattttt ggtaaggaag ttaaagcata cacagaatct
    29521 ttatatcaaa agattggtgt tttatttgaa tttccttatt tatataccaa tttaagtgca
    29581 attgataatt tagaatattt ttcgtctttt tatcctaaaa atcaaaggag agacatatat
    29641 gaactcttag atttgttaga gtttaagaaa gagtttataa ataagcctgt atcatcctac
    29701 tcaaaaggta tgaagcaacg tattagcatg gcaagagcat tggtaagtaa tccaaagttg
    29761 ttatttttag atgagcctac tagtggactt gacccatctg gagctgtact atttcgtaag
    29821 attattgaag aagaacgaaa aaaaggaaca actatctttt tgactactca taatatgcta
    29881 gatgcagatt taatgtgtaa caatgtggct tttattgcag atggaaagat tatggtaata
    29941 gataaaccca aaaatttaaa aatgaaaaat agcaataata aagttgaagt ggaatttgta
    30001 tataatggaa atcgagatat tgaaattgta gatatacaag agttagaatc tggaataact
    30061 tttaaatatg atgaaatctt aagtgtacat tcaaaagaac ctacactaga agaagttttt
    30121 attaaataca caggaaggat gttagtgtaa tgtattctaa attttttacc ttagtaaaaa
    30181 aagatttttg gatgatgatt tatggtaaat tttttgtgct gacacttggc tctctcatac
    30241 tttattcgtg ttatattaat tttggttatg taaagtttat ggatgaagat agttatcaca
    30301 ataatgtata tctatataat ccagagaata taagcatgag tgcatctcca ttaattaatc
    30361 aagtttcttc tataggtgaa ttaaaagcaa aacttgatga agatacaagt gcagtttgta
    30421 ttgatatgag ttccagtaag ccgaaagtaa ttttacatga aagtattaaa ggagttgaca
    30481 attatagagc tgactatgca ttgtcattat tatcatcaga aaaagaccat aatagcaaaa
    30541 tatcagacaa caatgcaata attcaaatta atgataagga tacatctgag ataaaacaaa
    30601 gaaaagacat gacttgtgag attttattct ttgaaattgt agctattgga tttttaggga
    30661 ttgcctcttt attatttaaa gaaaagcaga tgggagtaat gcgtattact ggtattttac
    30721 ctgtaaaaag aagtcttttt atattatcaa agatatttgc tttttttata tgtgacctat
    30781 gttttacagt tcttttagta gtatttaata ttggattttt agaaggaata aaaatacttc
    30841 cacaagtatt agttcaagct agtattttat caattattat gtctttgatt ggaataggat
    30901 gctcaatgtt attgagagat tttaagcaat tttctcttat atatcttgtg gttgctgtat
    30961 tcataactac acctatattt ctatctgcaa atacatcttt tgaaatagat tggattaaat
    31021 atcatccctt ctatcatgtt tatagtcagc ttaaaaatgc gtttttcatg aatttttcaa
    31081 aagaattagt ttattttgta gtgtgttctt tgacaataat agttctattt ttacttgtaa
    31141 aatatatttt taataaagaa ttggttaggg agggataatc ttgataggat tacgttacca
    31201 gttaaagagt ataaaaaaag ataaaatgtg tataatttcc ttctttttac cagttgttat
    31261 ggcgatttta ataaatttta tgggaacaat tgatatatct tcattaggag agatacgttt
    31321 tggtatagtg aaggatacac ttcctgtaca aacagagcag tggttgaaga agtatggtat
    31381 ggtaagtgta tatgacacaa aaatgcattt aatacgtgaa attaaggata ttaaaacgga
    31441 cacaattgga gttgttagag atggtaaagg aataaaaact gtgttttatg gaaatgagat
    31501 taatgctata aaaaatgttg gaaagacatt gccagagatg tttagactaa agggtgattt
    31561 aaaagatgtt gaggttacaa tattgcctaa agatagtatt ttaaaagagt ttcaaaatgt
    31621 ttttattgct atgattttaa ttactgctat gtttatggga tgcaccttta atgcaatgaa
    31681 tattatatcg gaaaaagaag atggaatatc atttataaat gaagttttgc cacagagtag
    31741 aagtcagtat attatacaaa aagtattttt gggtctttta tgtgggtgtt tatcttcaat
    31801 gataactgta tttatatgtt tggatatttc aatattagat atgataatga tgttgttact
    31861 cgttgtattt tcagcattta tttcatcact tataggttta tttattggaa agttgtcaga
    31921 aggtcttatg gtaggaattg tttatattaa aatagtaatg attttattta taggtactcc
    31981 tttgttaata tatcttctag gggttaatac cagtggtata gggaatttat gctatataat
    32041 gccttcagtt gctacattta atggtattat ggagattatt aatggtaatg tcattattat
    32101 caaagatatt gtaattttgg ctgtacattg tgtagtatgg tttatgatat atattatttt
    32161 agataaaaaa agaaagttat cttttagtag ataactcagt ataattataa ttgggacaat
    32221 ttaataggtg atttaatata ctttgtatag tgtaatttat gtagtgcaat ttgtatactt
    32281 taatttatgt agtgtaatag ttagttttat tttataatta ttcaatataa tttagtaaga
    32341 agttcagtac aattagagct acttagtgga aaacttaata ggtacttaaa ctatataaat
    32401 actttatttt cctccatttg tactataaat ttaatttatg atataaatgg aggtattatt
    32461 atatgatttg ataaatttca tcattataac ctacaagtaa aggtattatt atat
//
LOCUS       DAEMGG010000034        32491 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEMGG010000034 DAEMGG010000000
VERSION     DAEMGG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.034"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            197..1123
                     /locus_tag="KPK24_001535"
     CDS             197..1123
                     /locus_tag="KPK24_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG2299216.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(1506..3158)
                     /locus_tag="KPK24_001536"
     CDS             complement(1506..3158)
                     /locus_tag="KPK24_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG2299217.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            3757..4878
                     /locus_tag="KPK24_001537"
     CDS             3757..4878
                     /locus_tag="KPK24_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG2299218.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILIPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            4914..5327
                     /locus_tag="KPK24_001538"
     CDS             4914..5327
                     /locus_tag="KPK24_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2299219.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            5320..5859
                     /locus_tag="KPK24_001539"
     CDS             5320..5859
                     /locus_tag="KPK24_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2299220.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(6059..6544)
                     /locus_tag="KPK24_001540"
     CDS             complement(6059..6544)
                     /locus_tag="KPK24_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299221.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            7449..7826
                     /locus_tag="KPK24_001541"
     CDS             7449..7826
                     /locus_tag="KPK24_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG2299222.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            8222..10027
                     /locus_tag="KPK24_001542"
     CDS             8222..10027
                     /locus_tag="KPK24_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2299223.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            10104..10641
                     /locus_tag="KPK24_001543"
                     /pseudo
     CDS             10104..10641
                     /locus_tag="KPK24_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            11255..12469
                     /gene="dpaL"
                     /locus_tag="KPK24_001544"
     CDS             11255..12469
                     /gene="dpaL"
                     /locus_tag="KPK24_001544"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG2299224.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            12802..13989
                     /locus_tag="KPK24_001545"
     CDS             12802..13989
                     /locus_tag="KPK24_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG2299225.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            14005..15588
                     /locus_tag="KPK24_001546"
     CDS             14005..15588
                     /locus_tag="KPK24_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG2299226.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            15616..16944
                     /gene="ssnA"
                     /locus_tag="KPK24_001547"
     CDS             15616..16944
                     /gene="ssnA"
                     /locus_tag="KPK24_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG2299227.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            17089..18441
                     /locus_tag="KPK24_001548"
     CDS             17089..18441
                     /locus_tag="KPK24_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG2299228.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            18633..19742
                     /locus_tag="KPK24_001549"
     CDS             18633..19742
                     /locus_tag="KPK24_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2299229.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            20025..21416
                     /gene="hydA"
                     /locus_tag="KPK24_001550"
     CDS             20025..21416
                     /gene="hydA"
                     /locus_tag="KPK24_001550"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG2299230.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            21410..23971
                     /gene="xdh"
                     /locus_tag="KPK24_001551"
     CDS             21410..23971
                     /gene="xdh"
                     /locus_tag="KPK24_001551"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG2299231.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            24123..25469
                     /gene="rpoN"
                     /locus_tag="KPK24_001552"
     CDS             24123..25469
                     /gene="rpoN"
                     /locus_tag="KPK24_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG2299232.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            26267..27289
                     /locus_tag="KPK24_001553"
     CDS             26267..27289
                     /locus_tag="KPK24_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2299233.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            27349..28356
                     /gene="gap"
                     /locus_tag="KPK24_001554"
     CDS             27349..28356
                     /gene="gap"
                     /locus_tag="KPK24_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG2299234.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            28578..29780
                     /locus_tag="KPK24_001555"
     CDS             28578..29780
                     /locus_tag="KPK24_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG2299235.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            29806..30549
                     /locus_tag="KPK24_001556"
     CDS             29806..30549
                     /locus_tag="KPK24_001556"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG2299236.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEIKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            30558..32090
                     /locus_tag="KPK24_001557"
     CDS             30558..32090
                     /locus_tag="KPK24_001557"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG2299237.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT        12862 a         3684 c         6050 g         9895 t
ORIGIN      
        1 atttttatat taaaaataat attaagttta ttgtttaaat aaaaaagtta aatctacact
       61 attaacttat tatacttaaa actttaaaat gaaaatatca aagaatactt tttaataaaa
      121 ataatcattt aataagttat ttaaaaatct aaaaacttta caacaaatta tatatttaaa
      181 ttggaggagt gggaatatga gtaatagttt ttatcaatta aattcagcag tattatattt
      241 taatgaaaaa agagatgtaa atagaataag gtttaatcac tatgtaatga gaaaagaaaa
      301 tatacaaaga ttaaaaggaa ctctgccaag aggacaattt atgatgacag taaggaaagc
      361 tgcatctgac cttgatgtat ctatatctac aataagtaga cttgtaaatg aatttataga
      421 tttaggtata ttgagattaa taagtagggg agtaaaggga aattgttgtt ctgtatatag
      481 ttatgtttgt tctgaaagta gtaatgcaga aattgtaaca aagaatggtg atattttcaa
      541 aaatatagag aaatacattg atgcaactat taacaataac caaaaaacaa tgaaatataa
      601 acatagaaat gagttgttta agaacagtaa taccaacata tacagccgta taaatcaaga
      661 gagtatagac aaagattatg atttgataat tcatactggg tatgaaacag aaaatgagac
      721 aaagaaaaaa gaattattaa aaaataattt aaataaaaag aatttaaata aaaatacctt
      781 tgaaaattcg gatgaagatt tgactgaaga ttctacttac aaaaatccag aaagcataca
      841 tgaaattatt ataagaaaat taaatagtga atcaggaaaa aattttaaag ctgatttgag
      901 tataactaag gcacttatag ataaaagatt aaaagaaggg tatagtttag aagattttta
      961 caaggttata gaggcaaagg taagaagatg gagatataca gatatggaaa tatatataga
     1021 accaacaatt ctgtttgggt atgaatttga gtcgtattta aatgaggaga gttctttatc
     1081 tgcttgtaat tatggtgata tgtgttatga agatatgtat tgagatattt attaagatat
     1141 tagttgcttt ttagttgttt aaaatatgtt tatgtataaa tagtgtatta aaatggagtt
     1201 gtctcaaaat agagagaagt ctcttcttaa ttgtttaaat gatgaaaata tatttgattt
     1261 aatacaaagg agagtgcttt atgagttaga agatttattt tgagacactc acttttttta
     1321 atatactgga gaaatataac ccattttaca agagtgcata gatatatata atggcataat
     1381 gtggatttaa attaaaaaaa taaaataaag ccataaatat tgaaaatttc aatatttatg
     1441 gctttattgc ttgtaataat taatagaata tatatttttt attaatcatc tatttatatt
     1501 ttttattaat catctatttc aaattctaat attttaccag taattgcatt aattgttatc
     1561 tcatattcag tttttccttt tattatttca ccttcatatt ctttgtcatc ttcatcatac
     1621 ttaaatttta ctattttccc accaggcact ttatctagca ttattgattt agctttttca
     1681 gcactaataa tagaactgtt gtcattagta ttagagttat tcttatcatt atcatcaaag
     1741 tcatcatcag aatcattgtc catgtcaata tctacaattc ttccatcaaa tgcacttatc
     1801 tctaattcat atgttttctt attttttatt actttagcct catattcgtt atcttcagca
     1861 tcatatttac aatagattaa attaccatta ggaatagcat ctaatgctat agacttagct
     1921 ttatccaatg atatattttg tgtacctaaa ttattatcat ttaagtcatc atctttttca
     1981 atttctatta ttttaccaga gtaggcatca atttctatct catataggat attatccttt
     2041 ataactttgc agtcatattc attatcatct tcatcataat aacaaactaa aatattgctt
     2101 cctggaagag cttgttttaa tatttcttta gcctgtatta aagatatttt tttagtttta
     2161 tttcttatgt tcacattttt tagttttaaa atatcttcaa atgctttttg attatatcct
     2221 cctcctactt gtgttatttc atctatatct ttatttttaa taaattcttt ttgactattt
     2281 acaaggctac cattagatat cattatagga gaattttctt tagcagctag tactcctatt
     2341 gataatgaat ctactaatgc attttcttgg ttaataccat ctttagctac aaatacatta
     2401 tttatttttt tatctttata aaatttatca ataatttttg cattagtatt gtttctatct
     2461 gaaccactga ttctttctct gtttggatat ttctttactg tattgtctga taaaatctta
     2521 tttcctccaa ctatgtaaga tttttttata gacctattgt ttaagaaatt tatactacta
     2581 tcattgctgt tttcatctga tactaatatt ataggcatat tatgtattgc tgctggagaa
     2641 gctatactaa ctgcatcaga tagtcccttt agtccattta caactgcaac attttctatc
     2701 ttttttatct tatctatttc tttaagtaca tttgtagaca tttcatatat attgtttcct
     2761 actattttat ttatttttat gttaagtgat tttatttcat tttctacttt agaagaaatg
     2821 ctatttccac tatctacaat gtacacatta tctacaccaa gccttttaag ctcagcctta
     2881 gttgaatttt caatactgtt gttgtttact aaaagtatag gtgcattctt tagcttagca
     2941 aatggatttg cacaaagcgc actttgaata gaattaccat taactataat ggctgttttt
     3001 gattgaaccc atcccaattt acttacttct actgctgttt catatcttgt tttacctatt
     3061 aatctttctt gctttgtagt attgtctgca aaaattgctg aagaactaga aaaaaacatc
     3121 atacctgata ttaatatagc taaatttttc ttattcatat ttttacctca caatctttaa
     3181 aaatttgtta tttagcatac ttaataaaat ataatcttaa aatcaaattg atactgagtt
     3241 tgaagttaca aaatcaacaa ataagatttc tttaatacaa aattttcctt tttatattta
     3301 acattgtatc acaaatataa aaaggaaaaa actttttaaa aagggtatac actattcatt
     3361 ttttatctta taaataagtc tttatcactg ttttatatat tttaaaatag acacaagacc
     3421 tatttctaaa tcttttataa atattttagg tgaaatttct ttttaaacat atctaaaaag
     3481 tatttgtagt aatattaatg gactgaattt cttaaagtat atatttcttt acatgttgga
     3541 gtttgtgaag tttgagaatt ggagggtgaa agaaacatgt aatggattaa tattaaagaa
     3601 ctgtattttt ataatatttg tcttaaaaat aaaaaaatct attgacttga agttaactcc
     3661 aagtagtaaa gtataattat acaagaatta tatacttgta agaatagtta aagtagagag
     3721 aaaaaacaaa caagttttaa agggggaagt aaaacaatgg aggcaaaaaa attaggattt
     3781 ggattaatgc gtttaccaat aaaagatgaa aatgatgtta caactattga tatggaacat
     3841 ttaaataaaa tggtagacac ttttttagaa aggggattta catattttga tacggcctat
     3901 gtgtatcaca tgggaaaaag tgagatagca cttagagaat ctctagtaaa aagacacaaa
     3961 agagagagtt ttactatagc tacaaaactt ccattaatgg ctttaaagaa aaaagaagag
     4021 caagaaacca tttttaatga gcaattagaa aaatgtggtg tagactattt tgactactat
     4081 cttttacaca atataggcgt gtcacattat gaagtagcta aaaaatttga tagctttaag
     4141 tttattgaag aaaagaaaaa agaaggaaaa ataaagaata ttggtttttc ttttcatgat
     4201 agtgcagaat tattggataa agtcttgagt gagcatccag aagtagactt cgtgcaatta
     4261 cagattaatt atttagattg ggataatgaa agcatacaat ctagaaaatg ctacgaggtg
     4321 gcagagaagc ataataagcc tgtaattgtt atggagccag taaaaggagg tacacttgca
     4381 aagataccag aaaaagcaga gaaattattg aatgactaca atccagatat gtcaatttca
     4441 tcttgggcaa tacgttttgc agcaagtaaa gataatgtta tgatggtctt aagtgggatg
     4501 tcaaatatgg aacaattatt agacaataca ggatatatgc aaaatttcaa accattcgta
     4561 caagaggaat ataatattat agatgaagca gtagagatta tcaacgaatc aattttaatt
     4621 ccttgtactg cctgtcagta ttgtgtagaa ggctgtccaa aaaatatcgc tattccaaac
     4681 tattttgcgt tgtacaatgc agaaaaacaa gctcttaata ctggattttc aacacagatg
     4741 gtctactata ataattatac taaaacttat ggaaaggcct cagattgtat tgaatgtaaa
     4801 caatgtgaag aaagctgtcc tcaacacatt aaaattatag atgctttaaa gaatgtagca
     4861 gaaacctttg agaaataatt caatctatac aatatcaaga ggagaagata tgtatgacga
     4921 tttcagaagt tagtaaaaaa tacgagcttt cagcggatac tttacgatat tatgaacgaa
     4981 ttggtttaat accacctgta aatagaaata aaagtggaat taggtctttt acagaagaag
     5041 attgtgagtg ggttaatttc ataaagtgta tgagaggtgc aggtctttct attgagacat
     5101 taattgagta tgtagctatg tttcaacaag ggagcagtac tatcaaagct agaaaagaac
     5161 ttttaataga gcagagaaac caactagcaa aaagaataga agaaatgcaa aaaactttgg
     5221 aacgattaaa ttttaagatt gatagatatg aagaaggtat tattgagaaa gaaaaagtat
     5281 taaagagcag tagaaataag aaaatggagg cagtatctta tgagtaaaaa tgtattaata
     5341 gtatcagcta gtcctagaaa aggtggaaat tctgatttat tgtgtgacca gtttatacaa
     5401 ggggtattag aatctaaaaa tcaagtggag aagatatttt taaaagataa agatattaac
     5461 tattgtactg gatgtggtgc ttgttatgaa aaagaagcaa attgttcaca gacagatgac
     5521 atgggagaaa ttcttgaaaa aatgattggg gcagatgtga ttgttttagc gactccagtt
     5581 tatttttata ctatgaatgc gcaaatgaaa actatgattg atagaacttg ctctagatat
     5641 actgaaattt gtgataaaga tttctatttt atcgttacag cagcagatga aaatgtggct
     5701 gatatggaaa gaacaataga aggatttaga ggatttacat catgtctaaa tggttctaag
     5761 gaaaaaggta tagtatatgg tgtaggtgct tggaatgtag gtgatattaa gggaagtaag
     5821 gcaatgaaac aggcatatga aatgggtaag ttggcgtagt atgtttgtat aaaattgtat
     5881 aaaattgaat atattcttat catttcaaca ataaaaagag gcttatctgt attttgagac
     5941 agcctctttg tgctatatca tttattttta ttctatatcc ttattatacc ctttattttt
     6001 ttatgcctat ttaagtatct gttgttactc atatatttta ataattaccc ttaaaaaact
     6061 atacagattc ctgcataata ttattttctt tagtaattaa tttttctagg tttttccaat
     6121 ccatttcttc catatttgca aaataatgac cacttcctat gcaagtcagg taagactttc
     6181 catctaattc aaacgaagaa cctccaatac actttgaatt acatttctta caagtacgag
     6241 ttttagttat actatcttta atagttttag agcattcctc cacagcatct ttatatgtat
     6301 ttagatgaaa aagatttgct ttaactctcc attttttttc attacattca atagtaaata
     6361 agctacgttt tggtttctta gttgaatata ctattttgta agaacgagca cctgtttttg
     6421 gttcaatttt acaatctaat gatttaccaa gttcatgtaa atgacatgca atatcataat
     6481 ctttaccact tagtacttct aacatttctt ctgttgtttc tgctgttaat tcacttttgc
     6541 tcatattaaa aaaatccccc tatataaata ataatttcat atgaaaataa ttaatataag
     6601 tatattaaat aaaactatta aaatcaataa aattactgca atcttacatt attgggcata
     6661 gaatgagagc taatgattta ttagaaataa gacttaatag tatgaaaatt tacttagacg
     6721 tggtacaatt ctataaaaga tttaaaacta ggaaaggaga aagttgttga gatacggcca
     6781 atataacaat ttatttataa gttgtaaatt tattatgaga ttaattagat tgttaagtaa
     6841 tactatcaaa aggtagtttt ttggattttc agcaacagtg gtagatattg ataatttttt
     6901 ggttattatt atatttagct gttatatttt caataggctc tatgataact agggtaaata
     6961 aggtatgggt aaaggtttta ttatttataa tagtgggatt gtcaactata gttctttcat
     7021 atgtaataac ttgattatgc aaaattctat gaagcaggaa ccctgtgact ttagtcatgg
     7081 gaggttcaga atttaaggtt aaaaaataac tttaatctgt aaattctcaa aacaagaatt
     7141 ctcatgttgg taaatttcta gatatgagaa aaaaattgac atcataaaaa agtaatttta
     7201 gagcgttttt ttacgaaaaa attcagaagt aactattttg aatgacttca caaaatttta
     7261 tatataaata aagtttggca cgttaattgc tttaataaaa gttaacaaga cacaaataaa
     7321 cataatcatc taagtattga ttatttgaag ttttagattt gaagtttaat ctaaaggttc
     7381 gtattaatat tggtgtttag gaaattttat tttcctgtaa atataactta ataataggag
     7441 gtgaagtcat gtcaaaagta gtaatagcta cagagaaagc tccaggagca attggaccat
     7501 attctcaagc aattcaagtt ggaaacatag tttctacatc aggtcaactt ccaatagata
     7561 tgaagactgg cgagttagta tctgacccaa aagaagcaac aaaagcttct ttagataatg
     7621 taaaagcaat attagaacaa gctggtacaa gtatggataa aatatttaag actacagtat
     7681 tcgtaaaaga tttaaaggat tttaatgata ttaatgaagt atatgctaca tattttaaag
     7741 aaaatccacc agcaaggtca tgtgtacaag ttgctcaatt accaaaagat gcagttttag
     7801 aaattgaagc aatggcagta ttataagtat tataacaata aaaccaataa caaaactaat
     7861 aaaaattaaa aactaataaa aaaagtctac aattaaaaaa gtttatatta aaaaagtcta
     7921 tataaaaatc tatctaaata tttaactatt gagcttttct catataatgt atatttttat
     7981 gtcaaaaata tatttgttat atgaggaaag ctttttttta ttattgtatt gtgatttatt
     8041 ttattgtctg catattataa ttaaggttac tatataaaac aaacttttta taaattaata
     8101 ttgtctgttt taacgcaaat atataaataa gtaatgtttt atatatattt taaagagtga
     8161 ctatatgtta aaatatagct aaactgataa tttggtaaat taaattctaa tgggggaaac
     8221 tatgttaagt aaattaaaag aatttcaaca agaaatgata aaatacactg aaacagtagc
     8281 tagtgtgtta gatgtagata ttgaaatagt tgacgataga ttaataagaa ttagtggtac
     8341 aggtctgtat aaatcaaaga taaatgaaag cgttgttact gagggcttta tttatgacaa
     8401 tgttatacag acaggtcagg aattagtcgt attggatata tgtgataatc agctttgtat
     8461 agaatgttcc cattacatga aatgcctaaa taaagtaata atagcagttc caataaagta
     8521 taataatcga actataggag taattggtgc tattagcaca gataaaacta aaaaagtaga
     8581 aataagtgca aaaatagata attatttaaa atttgtcaat catatatgcg atttaatttc
     8641 tatgaaaata gaagaacatg aagcgtcaaa aaatagcagt agaaaaatgg atatgatgat
     8701 agaaatcatt gaaaatgttg aaaaaggcgt tataatacta gacataaata gcaaaatatc
     8761 ttatataaac aatatagctc taaaaaaatt agatatagat aaaaatataa tagaaaatat
     8821 agtaaatata gtatctgttg aatcttcctc aaatggacat gaattactag aaattgatat
     8881 tgataacaaa atttataata taaatgccaa aataattccg gtatatccat atataaatca
     8941 atacgacaaa ataataatat ttgacaagac atatatcaat cataaaggac atggaaaagt
     9001 gaatagtggc tggggaaata gtgatataga gtctataatt ggaaattcag aagcaatgtt
     9061 aaaagtaaaa gagagaacta agaaattagc aaagtcaaat tcaactgtac ttataactgg
     9121 ggaaagtggt acgggtaaag agctaattgc tagggctata catgctgaag gtagcagatg
     9181 gaacaagcca tttattgcga taaactgtgc tgctattcca gaaaatctcc ttgaaagtga
     9241 gctatttggc tatataaaag gtgcatttag tggagctagt agtggaggga aagtaggtaa
     9301 atttgaactt gcaaatgaag gtgttatatt tcttgatgaa ataggagatt tgtcaatgcc
     9361 tttacaagca aagttactta gagttttaca agagagaaaa tttgcgagaa ttgggtcaaa
     9421 taaacttata gatttagata taagagtgat tgctgctaca aataaaaact tattgaaatt
     9481 agtaaatgaa ggtaagttta gggatgattt atactataga ttgaatgtaa ttcctataaa
     9541 tttgccacct cttagggaaa gaaaggatga tattgaggca ataatgatga agtttgcttc
     9601 taaatattct ttagaattag gtattcagtt aaataaaata gaagaaaatg taatgaatat
     9661 gttgattaac tataattggc cgggaaatat aagggaactg gaaaatgcag ttgaatatat
     9721 gatgaatttg gttggagatg atggaataat atataaagat atgcttccat tagatatttt
     9781 aaactattat aatattaatg gaaatattta taaaaataag gatgtaaata taatatttga
     9841 agatgatata gttggaggga tagtagagaa tcaagaaagg atactttcaa ttaaagaatt
     9901 agagttgact tacataaaca agttgttgaa taagtatgga agagatacaa agacgaagaa
     9961 gaagatagca aaagacttag gcataggttt ggctacacta tatagaaaat tggaagagga
    10021 acagtagtaa tataatatta aaatgatagg tatttgtgtg tttttataga taggtatttt
    10081 tactttatgg tggaggtgtt attatgcaat atgcgataga gctatattat gataaaaaaa
    10141 cggaaaagca actttttgat ttatctaaaa aaatcgctga tgaaaaaatt agtacgaagt
    10201 acttagaatg gaaaacaaga cctcatctta ctttggcttg tttcaatgat gtggatgagg
    10261 cttgttgcat agacaagcta aaggggtttg cacaaaacca taaggtaata cctgcttaca
    10321 ttggttcagt aggcatgttt aatgatacta aaacggtgtt tgtatcacct ataatgaata
    10381 gcaatgtatc agtttcagag agaattatat gaggtactac aagattttga ttcaaatggt
    10441 tgggaatggt accaccccaa tcgttgggta ccccattgca caatagcact tactggagat
    10501 gatgaaagtg aggctttttt tcgggcaagt gatgtattac tgcgtgagtt caaaaaaata
    10561 agtggtgagt ttgtctcaat aggattggtt aaaattacat ttcctgtgga agaaattttt
    10621 acagtgaata tgaataggta actttaactc tgttgtggat gttatgaata ttttgtatag
    10681 tgcatttcat cagataaatg cgaagttgcg aatttcgaca gctcaatagt cgcaagtagg
    10741 gcacaatggt acacatattg cacttttgct tattgggggg acgaccatgt aaacttctgt
    10801 aagaagctat gaaaagattt aagccatttt ggaggctttg ggtaataaag aatatttggg
    10861 taataaagta tataaattta tagagtttat gcgtaagaag gttatagcca tatatttata
    10921 gaaaataaaa gtagtaatat atctgacaaa acaatagaca tataaaaaat aagagcataa
    10981 taaaggaaga attaagataa ataaacgatt tctcgaattg ataattctcg tattgataaa
    11041 aatttcccaa tatgagaatt ttttctttta taaatcttat ttttcacact caaaatttat
    11101 aaactttaaa gaaattttat aaaagtatgt aattccaaca taaaaaaatt gtttttagaa
    11161 taaataatat tggcacggta attgctttgc tataaggcac aaagataaaa aattaaaatg
    11221 acaccgaaaa atttgaaagg ggaatataaa ataaatgaga gaaataaaat ggaaattaaa
    11281 taacttacct aaatcagagg aaaaagaaaa aggaattgaa tttttaaaag atgaagagat
    11341 tgcaaaggct aaagcttttc atgaaagctt tccacaatat gaaaagactc ctttagttaa
    11401 attagataac ttagcaaaat cactaggcgt aagcggaata tatgttaaag acgaatctta
    11461 tagatttggc ttaaacgcat ttaaagtttt aggtggttct tactctatgg gtagatactt
    11521 agctcaaaga ttaaatacag atatatctga gttagggtat gacaaattga cttcgaaaga
    11581 aataaaagaa aaattaggag aaataacttt ctttacagct acagatggaa atcacggtag
    11641 aggagttgct tggacagcta ataaattagg tcaaaaatct gttgtattaa tgccaaaagg
    11701 ttcttctgaa tttagattaa ataaaatcaa aggagaaggt gcagatgcta gtataacaga
    11761 cttaaactat gatgatgcag taagattagc taatgattat gcagaagctg atgaccatgg
    11821 agtaatggtt caagatacag catgggacgg atatgaagaa ataccagcat ggataatgca
    11881 aggatatgga actatggcta aagaagctat agaacaatta aaagaatatg gagtagatag
    11941 accaacacac gtatttgttc aagcaggagt tggttcactt gctggtgcag ttcaagggta
    12001 tgttgcttca atatatgatg aatgcccaat aacagtagta gtagaggctg atgaggctga
    12061 ttgttactat aaatcagcag aagctggaga tggaaaacca agatttgttg gaggagatat
    12121 gccaactata atggcagggc ttgcttgtgg agaaccaaac actataggtt ttgaagtgtt
    12181 aaaaaattat tcatcagcat tcgtttcagc accagactgg gtatctgcta aaggtatgag
    12241 aatattagga aaccctctaa gaggagatga gaaagtaatc tctggagaat ctggagcagt
    12301 tacaactgga gcattagttt caatccttga aagtgaagat ttaaaagact taagagaagc
    12361 tttgaaacta gatgagaact ctaaagtatt attaataagt acagaaggag atactgaccc
    12421 tgataaatat agagatatag tttggaatgg tgaatgccaa agtaaataaa agacttaagg
    12481 aaaataaaaa cttaacaaat aaatgaaaaa tttaaggaaa ataaaaactt aaataagtga
    12541 aaactttaaa taaaataaaa actaaaacaa atgaaaaatc taaataaatg aaaaagtaaa
    12601 gcaaatgaaa aattcaaaat aaaaactaaa taaaattaaa taaaaccaaa taaaattaaa
    12661 ataaagtcta aataaaaatt taataaataa aatataaaga aataaaaaat caatagatta
    12721 agtgaaatgc taataaatta aaaaagtgaa atatcaaaat aaaacgtcaa caaactaaaa
    12781 attatatggg ggaatatcaa aatgttaaat gaaacaagaa aaaaacaatt aactgaagta
    12841 tgccaagact taataagaaa tgctagttat tcaggtcaag aagaaaacgt agtaaaagct
    12901 atagaagaaa attttgcttt actaggtttt gatagttggt caagagatag atatggaaat
    12961 atagtaggat gtataaaagg aaataaacca ggtaaaaaaa tattatttga cgggcatata
    13021 gatacagttc cagttccaga tgcaagtaaa tggagtgttc ctccttttga agcgagaata
    13081 gtagatggga aaatatatgg tagaggaact tctgatatga aggggcaagt aagtgctatg
    13141 atggctgctg tttcatactt tgcagaagat actaacaaag actttgaagg tgaattgtat
    13201 gtagcaggtg ttgttcatga agaaatattt gaaggtgttt cagctagaga gataagtaag
    13261 gctgttcaac ctgattatgt tgtaataggt gaatcttcag aattaaactt aaagataggt
    13321 caaagaggta gaggagaaat agtagtagaa acttttggta aaccagctca ctctgctaat
    13381 ccagaaaaag gtataaatgc agtttataaa atggctaatg taatccaaaa gatacaacaa
    13441 ttagtacctc caactcatcc agttttaggt gatggaatat tagttttaac agatataaaa
    13501 tcttctccat atccaggagc atcagtagtg ccagattatt gcaaagctac ttttgataga
    13561 agattattag ttggagaaac aagagaaggc gtattagctc caatacaagc tttattagat
    13621 gaaatgatga aagaagaccc agaattaaat gcaaaagttt cttatgcagt tgaaaaagct
    13681 gattgctata caggaaatac tatagaaagt gaaagattct tcccaggttg gttatatgat
    13741 gaagaagatg aatttgttca agcagcgtat aagggattaa aagaagctgg aatagattca
    13801 gaaataactc aatactcatt ctgtacgaat ggaagtcact acgcaggtga ggctggaata
    13861 agaactatag gatttggtcc ttctaaggaa aatcttgctc atacaataga tgagtatata
    13921 gaattagacc aattattcaa aggtgcagaa ggatattatg gtatattaaa atcagtttat
    13981 aaaaaataat taggagatgg ttttgtgaga actttaataa aacatgggct tatagtagat
    14041 ggaaataaaa caccagcata tgaaggagat attttaatag aaaacgagaa aatattaaaa
    14101 atatcacaag atttaaacga agaagctgac aaagttatag atgcaaaagg tagagttata
    14161 tgtccaggtt tcatagatac tcatagccat tcagacctag ttattttagt taatccatat
    14221 aatgaggtaa agattagaca gggaattaca acagaagtgt tgggccaaga tggtatatct
    14281 atggcccctc tacctcaaga gcatatatct tcttggagaa aaaatttagc aggtctagat
    14341 ggtgaaagtg atgaaattga ttggaaatat gaaacaacag agaattattt aaaaatgatg
    14401 gattacaatg gggtaggttt aaatgaaact tatttagtac ctcatggaaa cgttagaatg
    14461 gaagctatgg gacttgaaga tagacctgct actaaagaag aaattcaaaa aatgtgtgaa
    14521 atcacagaga gagaattaaa agcaggagct ataggattgt ctactggact tatatatata
    14581 ccttgtgcat actcattgac tgaagaaatt atagagatgt gtaaggtagt agcaaaatat
    14641 gacggagtat ttgtagttca ccaaagaagt gaagcagata caattttaac ttcaatggaa
    14701 gaaatcatag aaattggaaa acaatcagga gtaaaagttc atttttcaca ttttaaggtt
    14761 tgtggtaaag caaactggaa gtatatacca caagttatag agcttttaga aaaagcagaa
    14821 aaggaaggaa taagagtatc ttttgaccaa tatccatatg cagcaggaag tacaatgctt
    14881 ggagtagttt tacctccttg ggcacattct ggaggaactg ataagttaat agaaagatta
    14941 agtgatgaaa acgaaagagc aaaaatgaaa aaagatatag caaatggaat agaaggctgg
    15001 gataatttta tagaatttgc tggaatagac caaatatttg taacaagcgt aaaaacagaa
    15061 aagaataaag atacaattgg taagagctta ttagaaattg gaaaaatgag aggaaaagac
    15121 ccattagatg caacgtttga tttattaaaa gaagaagaaa atgcagttgg gatggtagat
    15181 ttctatggat tagaagaaca tataatagga ttcatgaaaa gagacgagca aaatgtgtgt
    15241 acagatggtc ttttagcagg taaaccacat ccaagagctt atggttcttt ccctaaaata
    15301 ctaggaagat atgttagaga gttgaatgtg ttaacaatcg aagaagctgt ttataaaatg
    15361 actaaaaagg cagctacaag ctttagtata aaagatagag gcgaattaaa agaaggatat
    15421 tttgcagata tagttatatt tgacaaggat acagttagtg gttgtgatga ttttatcaac
    15481 tctatgcagt accctacagg tatagattac gtaattataa atggaaattg tgttgttgaa
    15541 gaaggaaaat acaataaaat taaagcagga aaagtactta aaaattaaat cttagcttta
    15601 aggaggaata aatttatgat tttagtgggg aatgggaggc taataagcca agattcttta
    15661 aatccctata tggaagatgg atgtgtagta ataaatgata atctaattga agatataggt
    15721 actacagaag aaatgaaagc taaatatcca gtacatgaat ttatagatgc aggcaagaaa
    15781 ataatcatgc cgggtctaat aaattcacat atgcatatat acagctcatt tgctagagga
    15841 atggcagttc caggaaaacc ttctgagaac tttttagaga tactaaataa tttatggtgg
    15901 agacttgaca aacagcttac actggaagat acaaaataca gtgcatatgc aacgtatata
    15961 gaatgtataa aaaatggtgt aacaacagtt tatgaccatc atgcaagtcc aaatgcaatt
    16021 gaaggcagtt tgtttacaat atcagatgtt gcaaaggatt tgggaattag aacgtgtctt
    16081 tgttatgaag tgtctgatag agatggtgga aatacaatcc aacaaggtat caatgaaaac
    16141 gtaaacttta taaaatacgc tcaaaaagat gatacagaca tgataaaagg aatgtttggg
    16201 cttcatgctg catttacact atctgatgat acattaaata aatgtgcttc agagatggct
    16261 ggtcttgata caggttacca tgtacatgtt gctgaaggac tagcagactt acaatataat
    16321 gtagataaat atggaaaaag ggttttagaa agattcaatg attttggagt actaaaagaa
    16381 aaaacaatag cagttcattg tatatatata agtgcagaag aaatcagtct tttaaataaa
    16441 acaaatacat ttgtagttca taatccagaa tctaatatgg gaaatgcagt tggatgttct
    16501 cctgcaatag agttattaaa aaggggagtt acagtaggat taggaactga tggatatact
    16561 agcgatatgt ttgaatcatt aaaagttgcc aatataattc acaaacatca cttaaaagac
    16621 ccaagagttg gatttgaaga atcacaaaca atgttatttg ataacaatag aaaaatagct
    16681 aaagcttatt ataaaaatga cctaggagta cttaaaaaag gtgcttatgc agatgttata
    16741 gtagttgatt atataccaca tactccattg acagaaaata caataggggg acacactatt
    16801 tttggaatga gtggaagaag cgtagataca actattataa atggaaaagt acttatgaaa
    16861 gatagaaagt taataaacat agatgaagaa agtattctag caaaaagtag agaattaagt
    16921 caaaatctat ggaatagaat ttaaataatt gatattaata tacaagtaag aaacaagaat
    16981 ttagatgtta aaaacagagc ttaaacaaaa ataggaattt aaatgaagta tataaataaa
    17041 aaatatacgg aatagaatat taaattaaat atagaaggga gacccaaaat gtctcgagat
    17101 aaaagcccta aaatagctcc agtagatgaa aggttacctg tatccaaatc ttgggtattt
    17161 tcactacagc acgttttagc aatgtgtgca ggagcagtag cagttcctat gatgatagga
    17221 agtgctgcac gactagatca tgctagtatt gtttttttaa taaatgcttg cttgtttatg
    17281 gcaggtatag gaacactcat acaaagtgtt ggtataggaa atattgtagg tgctaaaata
    17341 ccagtaatag aaggaactag ttttgccgct gtggcaggta tgacagcaat agcctcatca
    17401 tatagtgatt cacaagttgc aatacagaca gtatttggct ctgtaatagt agctggattg
    17461 ttttgtatat tacttgctcc agtatttgaa aaattaataa gatttttccc tagtgtggtt
    17521 actggaacag ttgttatggt aataggtatt tcactatttc cagttgggat aaaatggata
    17581 actgacaaca aattggaagc ggttgaacca aactcattag cattagcttt agcagtattg
    17641 gcaataactt tactttcatt taaattctta aaaggaattt ggaatagcac agctatattg
    17701 gtaggtatag tacttggtac actactttcc atggcagttg gaatggcaga ttttagtgca
    17761 gtaaatggag ttggttggct taacttaaat gtacctttaa aatttggaat gcctagattt
    17821 gaaccatctg caatactatc attgtgtttg attatgttgg tacttatgac agaatcagtt
    17881 ggtaatatga tagctattca tgaaatggta gacaaagaag taacaggtaa aaatattaaa
    17941 agaggtctat ttggagatgg aatatctaca gcattatctg gaatattcaa ttcatttcca
    18001 ataactccat ttggtcaaaa tacaggaata gtaggtctta caggtataaa atcaagattt
    18061 atagcagttt acgcaggggt tatattgcta gtacttggat tcttaccaaa atttgcagca
    18121 ataataggag caatacctaa gcctgttctt ggaggcgtag gatttgcaat gtttggaatg
    18181 gtaatggttg gaggagtaaa aactttaagc aaagtgaaat ttgatggaac taaaaatggg
    18241 gttatagtag cagttagtgt tggtttgtct atgataccac taacaaatcc tgatttctat
    18301 cataatttcc caacttgggt acaaacaata ttccatagtg gaataacaac aggtagttta
    18361 tcagcagtgc ttttaaacat attcttcaat gaactaggtt ctaaaaaaga aaaaaataat
    18421 aaagttgaag aagcaaaata aaaataaaac aagtaaaaat ctatattgac ttaaagtcat
    18481 taagtattaa taaaaataat tcagagatat taataattca acaaaactat cttatatttt
    18541 aaataatata aaaaagtaaa taaataacga atacaaataa aagaatacaa tctaaatatt
    18601 aaaaatattt aatttaaggg agagataata atatggctaa tttaaaagtt aatttatatg
    18661 ggatggaatt tttaaaccca attatgacag cggcaggacc tggagcaaaa gatggtgctc
    18721 tctgtcaaga ggcagcaaaa ggtggagcag gagctatatg tacaaaaact atatctgtat
    18781 taccagctga tgtaccaaga ccatgtatgg caaaaactaa tagtggtttt ttaaacacag
    18841 aattatggtc agagcttcca aaagagcaat ggatagagga agaatacaaa aaggtaaaag
    18901 aaacagggct tccagtaata gtaagtatgg gatattcagc agagcaaata gctgaggttg
    18961 ctcctttagt aaagccttat gcagatgcag ttgaattatc tactcattat gtaggtacag
    19021 acgttgcacc aatagtaaat gctttaaaag ctgcaaaagc tgttttagat gtaccagtat
    19081 ttatgaaaat gagtcctcat actgacattc aaactattgc aaaagctgtt gaagaagcag
    19141 gtgcagatgg acttgtaatg ataaactcat ttggaccatg tatggcaata gatgtaaata
    19201 caggataccc tataatggga agtaaagctg gatacggatg gttatcaggt gcaccaataa
    19261 gacctctagc aataagatgt atatatgaag cttctcaagt tgtaaatata ccaataatcg
    19321 gtgttggtgg aatcacttgt ggaaaagacg tggctgaaat gatgatggct ggagcaagtg
    19381 ctgttcaagt atgtacagaa gcaatcctta aagggccttc tatctatgga aaaattgcta
    19441 aagaattaaa tgaattttta gatgagaatg gatatgctga tgttaatgaa ataaaaggat
    19501 taactcataa gaaaatggca gctagagggt ttagaactca ttcagtagct ccagtagtgg
    19561 atgataacaa gtgtatcaaa tgtaagcaat gtgtaacaag ctgtgtatac gaagctttag
    19621 aagttacaga taagttgaac atagatgcag acaagtgttt tggatgtgga ttatgtgtaa
    19681 ctagatgtcc aaaaggagct ttaagcatct gctacaatca agtagaagct atgaatgagt
    19741 aataattata tagaattttg gtaattaact tagagtaata ggtattagga taataaaata
    19801 tatcagataa acaaacacac attatgcatt aataatacag tctaaagtta ataattttat
    19861 tatattgatt taactaaata ttagtttagc tgaatattaa tttaattagg tattggttta
    19921 atcttaatat agatttacta cgtataagtg aactaactag attactttaa gttaattttt
    19981 tcatataaaa taaattgata cattacaagt aaggtggagt ctatatggga attgttttaa
    20041 aaggtgggaa agtagtaaca agtgaaaaaa cttactgtgc agatgtaaga atagaagatg
    20101 aaaaaatagt cgatataggt cttgatatat caaaagaaaa tgatgaaata gttgatgtta
    20161 gtgattctta tgttatacca ggaggtatag atacacacac acactttgac ttagatacag
    20221 gcgttactaa gacagcagat aactttgaga caggaacaaa agctgctata gtaggaggaa
    20281 ctactactat attggatttt gctacacaag ctaaaggatg ttctttgaaa gacggattag
    20341 aagaatggca tgaaaaagct aaggataaat catattcaga ttatggcttt catatggcta
    20401 taacagattg gaatgactcg gtttgtaatg aaatggagga tatggtgaaa gagggagtat
    20461 cttcatttaa actgtatatg gcgtataaag gaagccttca agttgatgat ggtgtaatct
    20521 ttgaagcatt gagaaaagca gaagagatag gagggattat tggtttccat tgtgaaaatg
    20581 gggacataat atgtgaactt gtggataaag caaagtcaga aaataattta tctccaaaat
    20641 atcatcaatt aactagacca gctataatgg aggcagaagc tacatcaaga cttatgaaga
    20701 tagctgaagt tgcaaactct aaagtatatg tagttcactt aagctgtaaa gaatccttag
    20761 aggaggtttt aaaagctaag caaagaggtg tggatgtaat agttgaaact tgtcctcaat
    20821 accttctatt aaatgatgaa ttatatgatt tagatggatt tgaaagtgct aagtatgtaa
    20881 tgtcaccacc tttaagaaat aaagaaaata atgaaatatt atggaatgct attttagata
    20941 atcaaataga cactatagga acagaccatt gttcttttaa ttataaggga cagaaggaac
    21001 ttgggataaa cgatttcagt aagataccaa atggttctcc aggtgtagaa cataggatga
    21061 gtcttttata cacatatgga gtctgtaaaa ataaaatatc tatcaacaag atggtagaag
    21121 taacatcaac taatgcagca aagttgtttg gaatgtatcc taataaagga actatagaaa
    21181 taggcagtga tgcagatata ttagtcttaa atccaaacaa aaaatccaaa atagaagcaa
    21241 aaaaacaatt acaaaatgta gattatacac cttatgaagg gtatgaagtg gattgtcaaa
    21301 tagaacatgt ttatttaaga ggaaaagaag tagttagaga tggagaatta atttgtaata
    21361 ctccaagtgg gagatatata aataggcgtc taacaaaggg ggctagagaa tggtagaatt
    21421 tatattaaat ggagagaaag ttgttgcaaa agaaggcaaa aaacttttag acttccttag
    21481 agaagatatg aatataactt cagttaaaaa tggatgttca gaaggtgctt gtggaacatg
    21541 tatggttata atagatggta aagctactaa agcatgtgta tttaaaactg ataaattagc
    21601 tgggaaaaac ataacaacta tagagggatt atcagagaga gaaagagatg tttttgcata
    21661 ttcttttgct agtcaaggag ctgtacaatg tggattttgt attccaggta tggtaattag
    21721 ttcaaaagca cttatagacg ttaataataa tcctactgaa gatgatataa agaaagcttt
    21781 aaaaggcaat atatgtagat gtacagggta tgtaaaaata attaaagctg tcgatttagc
    21841 ggcaaagatt tttagagaaa atatagatgt gccaaaagta aactgtaagg gtttagtagg
    21901 tgaaaatctt cctagagttg atgcagttgg caaagcactt ggatattctg aatatgtaga
    21961 tgatatgaga attgagggta tgatttatgg ttcagcagtt agagctaagt atcctagagc
    22021 tttagttaaa aaaatagata tagaagaagc aaaagcttat ccaggagttg ttgcagttct
    22081 aacagctaaa gatattccag gaactataaa ggtagggcac ttaaagaagg attgggatac
    22141 tttaatacca gaaggtgaaa taactagata cttaggagat ggaatagttt tagtagcagc
    22201 agaaactaga gaagcgttag aagaggccaa aaagctagtt aagatagatt atgaggaatt
    22261 aacaccatta agtactacag cagatgcact tagagaagat gctccaaaga tacatgagac
    22321 agggaatata cttgttaagg aacatttagt aagaggaaac gctaaagaaa agatagaaaa
    22381 ttcaaaatat gttgtaacta aaagatatac tacacctgct acagaacacg catttttaga
    22441 gcctgaatgt gcagtagctg gtccttatga agatgatgga gtaataatct attcaacaga
    22501 ccaaggggtg tttgccactc aacatgaatg tgcagatatg ttaggattac catatgagaa
    22561 ggtaagagtt ataaacaaaa tggtaggagg agggtttggt ggaaaagaag acatgagtgt
    22621 tcaacaccat gcagccttac ttgcatatca tacaaagaga tttgtaaaag tacagttaac
    22681 aagaaaagaa agtattattg ttcatccaaa acgacatgcc acagaaatgg aatttacgac
    22741 agcatgtgat gaaaatggat acttaacggg tatgaaggca acgataatat ctgatacagg
    22801 tgcatatgcc tctcttggag gaccagtgct acaaagactg tgtactcatg cagcaggacc
    22861 atacaattat caagatattg atataacagg aacagcagta tatactaata accctcctgg
    22921 aggagcattt agaggatttg gagttacaca gagttgtttt gcaactgaat gtaatctaaa
    22981 tttacttgct gaaatggttg gaatatctcc ttgggagata agatataaaa atgcaataag
    23041 acctgggcaa gttttaccaa atggtcaatt tgcagatgaa ggaacagcaa tggcagagac
    23101 actagaagct gtaaaagaag agtatgaaaa gcatccacgt gcaggcatag cttgtgctat
    23161 gaaaaatagt ggggttggag ttggtatacc tgatgttgga agatgtagat tggtgataaa
    23221 agatggcaaa gttcacataa gaacaagtgc ttcttgtata ggtcaagggc ttggtactgt
    23281 tatgactcaa gtagtttgtg aaactgctaa cctacttcct gaaaatatag tgcatgaagt
    23341 tccagatact catttaacac cagactctgg taatactacg gcttcaagac agacagtatt
    23401 tacgggagaa gctactaagg ttgcatcaaa taagttaaaa gaagctttag actctggcaa
    23461 aactttactt gatttagagg gagaagagtt ttatggagaa tatgaaagta ttacagataa
    23521 aatgggttgt gacaaagaaa atccagtaag tcatgttgct tatggatatg caacacaagt
    23581 tgtaattctg gatgaaaatg gaaaactaga aaaagtagta gcagcacatg atgttggaag
    23641 agctataaat ccaatcagtt tagaaggtca aatagaaggt ggagtagtta tgggtcttgg
    23701 atatgctcta accgaagatt acccagtagt caactctgtt cctacagcta agtttggtac
    23761 attaggttta tttaaatcta cagatatacc agaaatacaa gctttaatag ttgaaaagaa
    23821 caaagattta ttggcttatg gggcaaaagg tgtaggagaa atttgtacag ttcctactgc
    23881 accagcagta caaaatgcct attatgtatt tgataaagaa tttagaactg agctaccact
    23941 acaaaatact ccatatagta aaaaaaagta acaaaaaaaa gaaatattaa ataaaaaaat
    24001 agatgaaatt gtaaataaaa aaaacccttt tgcataaggt atattttaaa tgatagaata
    24061 tacatataca ggagggtttt tcttttataa aataagttta ataaaattga gaggtgagca
    24121 gaatgaattt taataatagt ctagaattaa ctcagtctca gaagctaata atgactacac
    24181 aacttaagca atcattatca atattaaata tgagtaagtt agagttagaa gaagagataa
    24241 aaagagaagc agagaataac ccattagttg aagtcgagaa aagtggggag ataaattggg
    24301 aagagtatat aaaagacatg gataggtcaa gacgtttaga tagaacagag ataagctata
    24361 atcctgataa tgaagttaat ctagaaaatt tagttaaata ttcgtcaaat ttatatgaag
    24421 atttaaaatt tcagataagc ctgtataaac ttacagacaa agaattggaa gtctgtgagt
    24481 atataattga tagtttagat gaggatggat atttaagaac tgaggaaaaa gtaattgttg
    24541 ataccttaaa gatagatgaa gaactttttg aaaaatgttt aattagtgtt cagcagttgg
    24601 aacctagtgg agtaggggca agaagtcttt cagaatgtct gatgatacaa atggctagtt
    24661 taggaatata taatgaaatt ttagaggaaa tcgttacaaa agacttaaat ttaattgcaa
    24721 ataataaata caaagagata agtaaaaagt acaatatgtc attacaaaaa tgtgttgaac
    24781 taataaacat aattaagaca ttagacccca aaccaggaag aacttgttca gtagagaaaa
    24841 gtgtatacat tcaacctgat gtaacagtag aaaaaattga tgatgaattt attgtatatt
    24901 taaatgagaa agattcttat caaataagaa tcaatagtta ttataaagac atattaaaaa
    24961 attcacagtc tgatgaaagt gcaaaagaat ttataaaaga aagactaaat tcagcaacag
    25021 gacttatgaa aaatatagag agtaggaaaa ctacagtact taagattgca gaagagatag
    25081 taaaagctca agatgaattt ttgagaaaag ggacaaaata tataaaacct ttgaaaatga
    25141 aagacatagc agaaaaatta gaatttcatg aatctactat aagtagaggt gtaaatggta
    25201 aatatatgct aacacctttt ggcgtatatg aatttaggta ctttttcagt agcgccattg
    25261 agactgagaa taatgaaatg gcatctagta caagcataaa aaaaattata aaggaaacaa
    25321 taaaagacga aaataaaaag aaacctttaa gtgatgacca tatatctaag ttattaaaag
    25381 aaaaaggaat caatgttgca agaaggacag ttgccaaata tcgtgaagaa ttaggaatcc
    25441 catcttcaag taagagaaaa gaatactaat aagtaaaaga caaatatcag tatggtaagg
    25501 ggaaaaggct aattaagaga tgaatgtaga ttgggctaaa aattttataa agtaaaataa
    25561 tagaatatgg aaaagtatta aaatgctata aggtattaaa aaactgggga acattaaaac
    25621 attaagatag caaagaacat aaaaaactaa agaagattaa aacattaaag aacattagag
    25681 ctttgaggaa tattagaact ttaaagaata ttaaaattta taaaatataa aaatactaaa
    25741 aagaattgaa aaactgggga acattaaaat attgtaaaat gctaaaaaac tagaaaacat
    25801 aattaaataa gtgcttataa agaatgatta aattaaataa gtgtttgtaa agaataatta
    25861 gatattagaa aaagaagaat acctagataa ataaactatt attaaattta ataatcacaa
    25921 aaggaaaaca cgtagggtat aaataaaaat cttaactaga taaaagtaaa cttagttgta
    25981 gataaaaata actagaaata aaagtaacta gaaataaaat tatttatagt aataaatatt
    26041 agaatgaaaa tataaaaaaa tatatataaa aagccttata taaaaagaaa aaatactgtg
    26101 atttgaaaat ggtggagaaa tattttataa aataaaaaaa taaaaaagtt tttgaaaaac
    26161 tattgaaatc tatacgcaaa tcctataaaa taaaaatatc ttgtgggact taaataaaca
    26221 ctagggacaa aaaaagtccc gaaaggaaag caataaggag tatgttatga agaacttatt
    26281 agaaattcaa aagaaattga ttccacaggc aattgaactt atggaaagaa gatactctat
    26341 tttaagacaa atatctttaa gtgaaccaat aggtaggaga acattatcaa atgtattaga
    26401 gattagtgaa agaataatca ggtcagaaac tgagttttta aaagaacaag gacttataga
    26461 cgtagctgta tctgggatga ctatcactaa agaaggttct gatttgttag ataaacttaa
    26521 ggacatcatg agtgacatca tgggattgtc taagttacaa gatagagtca gagaaaagct
    26581 tggaataaaa agggtaatat tagttcctgg aagctgtgat gaaaatggaa gtttactaaa
    26641 agatgtaact agatatggat gcgaatattt cttaggcatt ttaaaagatg gagatatcgt
    26701 ttcaataaca ggaggaagca ctatgttgga gttttcaaac gttattaaaa ctgataagag
    26761 atatccaaac tctacaatag ttccagctag aggaagtatg ggaactgatg tagagatgca
    26821 atctaacaat atagttgctt caataagtaa gaaattacat tctaattgca aacttttaca
    26881 tataccagat gagttggatg aaagtgcaat gaaaactctt agccaagtgc cagagattaa
    26941 gacaactttg gagtatatta aaagaacaga cattttagtt tttagtatag gtagagcaga
    27001 tgtcatggca aagagaagaa aacttccaga agacaaggtt gatgagatat tatctaaaaa
    27061 tgcagttgga gaagcatttg gatattattt caacaaagat ggagaaatag tttacaaact
    27121 gaacactgtt ggaattgatt tagaaacagt taaacaagta aaagaaacta ttgcaatctt
    27181 tgcagggact gaaaaagtag aagccttatt agctatatca aatatgcata aaaacatggt
    27241 tcttgtaaca gatgaagaaa gtgcttataa gatattgtca ttattataaa agtcaagacc
    27301 tagcacagag ctagaaaaca tacatttact ttataggagg tagcgaaaat ggttaaagta
    27361 gctataaatg gatttggaag aataggaaga ttagcattaa gaaaaatgat ggagcaacaa
    27421 gataagttcg aagttgttgc aataaacgac ttaacagacg ctaaaatgtt agctcattta
    27481 tttaaatatg atacagctca aggaagattt aacggagaaa tagaagttaa agaaggtgct
    27541 tttgtagtaa acggaaaaga aataaaagta acagcagaaa gaaatcctgc tgacctacca
    27601 tgggctgaat taggagtaga tatagtatta gaatgtacag gattctttac atctaaagat
    27661 aaagctgaag ctcatataca agcaggggct aagaaagttg ttatatctgc accagctact
    27721 ggagatttaa aaacaatagt attcaacaca aacagtgaca tattagatgg ttctgaaaca
    27781 gttatatcag gagcttcttg tactactaac tgtttagctc caatggctaa agtattaaat
    27841 gataaatacg gtatagaaaa aggattaatg actactatac atgcatacac taatgaccaa
    27901 aatactttag atggtcctca tccaaaaggt gatttaagaa gagctagagc tgctgctgga
    27961 aacatagttc caaacactac aggagctgca aaagcaatag gcttagtaat accttcatta
    28021 aaaggaaaat tagatggggc agcacaaaga gtaccagttg taactggttc aataactgaa
    28081 ttagtttgta cattaggaaa aaatgttaca gttgaagaaa taaatgcagc tatgaaagaa
    28141 gcttcaaacg aatctttcgg atatactgaa gaaatgttag tatcttctga cataatcgga
    28201 ataagctacg gttcattatt tgacgctact caaacaaaag ttatggaagt agatggaaaa
    28261 caattagtta aagttgtttc ttggtatgat aatgaaatgt cttatacttc tcaattaata
    28321 agaactttag gatactttgc acaattagca aaatagtcag ttttgagaaa atagaaaagt
    28381 agattgacaa agtccgagtt tgtagtttaa tactacggac tcaggacttt ttttaagaaa
    28441 aaatatataa aatacaccta aataatgtta catactatat ataaaaatat gataatatga
    28501 tatatttaat agggtaaaag tatgtactat attatacttg gcaataaaaa attactttat
    28561 taaaaggaga gatatttatg tcaatgctta acaaaaaaac tatagaagat attgatgtgt
    28621 gcggaaaaaa agtattagta agatgtgact ttaatgtgcc attacaagat ggtgtaatta
    28681 ctgatgaaaa tagattgaac ggagctttac caactattca atatttaatt tctaaagggg
    28741 caaaagttat attatgctct cacttaggaa agccaaaagg agaagctaaa ccagaattat
    28801 ctttagcacc agtagcaaag agattatctg agatgttagg aaaagaagtt gtatttgcag
    28861 cagacgataa tgttgtagga gaaaatgcaa agaaagcaac tgaaaaaatg gaaaacggag
    28921 atgtagtatt attagaaaat actagatata gaaaagaaga aactaaaaac gaagagaatt
    28981 tctctaaaga actagcatca cttgctgaaa tatttgtaaa tgatgcattt ggaactgctc
    29041 atagagcaca ttgctcaaca gttggtgcag gcgagttttt acaagagaga gtttgtggat
    29101 acttaattca aaaagaatta aaattcttag gagaagctgt tgcaaaccct gtaagaccat
    29161 ttacagctat acttggagga gctaaagttt ctgataaatt agctgttata aacgaattat
    29221 tagaaaaagt tgataatcta ataataggtg gaggaatggc ttatacattc ttaaaagctc
    29281 agggttatga agtaggaact tctttacttg aaatagataa agttgaatat gctaaagaaa
    29341 tgatggaaaa agctaagaat aagggtgtta acttattatt accagtagac gttgttatgg
    29401 cagaccactt tgcaccagat gctactccaa tagtaacaga agatgctaat gtaaaagaag
    29461 attacatggg actagatatg ggacctaaga ctatagcaaa ctttgtaaaa actataaaag
    29521 aatcaaaaac tgtagtatgg aatgggccaa tgggagtatt tgaatttgaa aactttgcaa
    29581 atggtacatt atcagtagct agagctatgg cagagttgac tgatgcaact actgtaattg
    29641 gcggtggaga tagtgcagca gcagttaatc aattaggatt tggtgacaag atgactcacg
    29701 tatcaacagg tggaggagca tcactagaat ttttagaagg taaagaatta ccaggtattg
    29761 cagcattaga taataaataa actaatatat agaggagatt gtaaaatgag aaaacctata
    29821 attgcaggaa actggaaaat gcataaaaca ataaaagaag ctttagaatt tgtaaatgaa
    29881 attaaagata aagtaaattc tgataaagta gaagctgtta tatgtgcacc atttacttta
    29941 ttaaaggatt taaaagaagc tactaagggt acaaatataa agataggtgc tcaaaatatg
    30001 cactttgaag aaaaaggagc atttacagga gaagtttcac ctcttatgtt aaaagaaata
    30061 gatatggact atgttgtaat aggacattca gaaagaagac aatacttcaa tgaaactgat
    30121 gaaactgtaa acaagaaagt tttaaaagca ttagaagtag gaatagaccc aatattatgt
    30181 gttggagaaa ctttagaaca aagagaagct ggaaaaacta aagatgtttg taaagttcaa
    30241 gttgaaaaag ctttagaaaa tgttcttaaa gatgatttag caaaagtagt tgttgcatat
    30301 gagccaatct gggctattgg aactggtaaa actgcaactg ctgaagatgc taatgacgtt
    30361 atatcttata taagagaagt tataaaagga ttatatggag aattagctaa tgaagttaga
    30421 atacaatacg gtggaagtgt taaaccttca aatgtagccg aaataatggg tcaaagtgat
    30481 atagatggag ctttagtagg tggagctagt ttagctagta atgattacct agaccttgtt
    30541 aatttctaag gagtgaaatg atgaaaaaac cagttgcttt aattattatg gacgggtttg
    30601 gatacaataa ggatgttaag ggaaatgcaa ttgctgagtc caaaactcct aatttagata
    30661 gaataaaaaa agagtatcca aacacattga taaatgctag tggtcttgat gttggtctac
    30721 ctgatggtca aatgggaaat tcagaagttg gacacactaa cataggtgca ggtagaatag
    30781 tatatcaaga tttaactaga ataacaaaat ctataaaaga tggtgacttt tttactaata
    30841 aagttttatg tgaagcaatg gataatgcaa aagaaaattc tcttcatgta atgggacttt
    30901 tgtctgatgg aggagtacat tcacatatag accatcttaa ggctataatc aaaatggcaa
    30961 aagataaagg cgtacaaaag gtgtatgttc acgcatttac tgatggtaga gatactgacc
    31021 ctcaaagtgc attagaatat gccaaagaag tacaagctag tatggatgaa attggagtag
    31081 gtgaatttgc tacagtttct ggtagatact acgctatgga tagagataag agatgggaaa
    31141 gagtagaact tgcatacaat gccatggtta gaggtattgg tgaaaaagca aattctattg
    31201 aggaagctat tcaaaactct tatgatgatg gaaaaaatga tgagtttata atgccaacag
    31261 ttattatgaa agatgataaa ccagttggaa gtataaagga aaatgattca attatattct
    31321 tcaactttag accagataga gctagacaaa taactagagc tttagtttgt gaagagtttg
    31381 atggatttaa aagagaagat attaaaaact tctttgtatg cttaacagag tacgatataa
    31441 ctatagagaa tgtgcatata gcatttggac ctcaatcttt agccaacact ctaggtgagt
    31501 atctagctaa aaatggaaaa actcaactta gagctgctga aactgaaaag tatgcacatg
    31561 taactttctt cttcaatggt ggagtagaag agccaaataa aggtgaagaa agactattga
    31621 taccttctcc aaaggttgca acttatgatt taaaaccaga aatgtcagca tatgaattga
    31681 cagataaagc tttagataaa ttaggagaag ataagtttga ctttatagta ttaaactttg
    31741 caaatcctga tatggtaggt catacaggaa gtatagaagc ggctataaag gctgttgaaa
    31801 ctgtagatac ttgtgtagga aaattgatag ataaaatagt agagttaggt ggaagtgcaa
    31861 taataactgc tgaccacgga aatgctgaat atatgttaga cccagaaaca ggaaaaactg
    31921 taacagctca ctctattaat ccagtaccat ttattgtagt tggtcaagaa tatgaaagtg
    31981 caaaattatt ggatggagga agattatctg atatagcacc gactgtttta gatatgatga
    32041 agttagaaaa gccagaagaa atgactggtc attcacttat ttcaaaatag tcgattttga
    32101 taagtagaga ataatatata aactacaata taaatttaaa aataatgcag tttaggttta
    32161 ttttgtaaag tttagacttt atgacataag gtttaaattt tgtaagttct ggatattgta
    32221 aggtgtagat gaagatatat tttataaaat ttacaagata gacaatttat aaaataaaaa
    32281 attacaatat agataataat gtaaataatg atataaataa taacaagata gacaataaca
    32341 atataaataa taataattaa tatatattat ataagcaaaa attttagaat cattagttgt
    32401 acattttatg tttaatgttt taaatctagt tataatattt gtgtatttaa gattggtttt
    32461 ggattttaga caagtttttt tatattatat a
//
LOCUS       DAEMGG010000035        32391 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEMGG010000035 DAEMGG010000000
VERSION     DAEMGG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.035"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..884)
                     /locus_tag="KPK24_001558"
     CDS             complement(<1..884)
                     /locus_tag="KPK24_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG2299238.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQTMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPSS
                     LDTESSIKQGCKYFASLLSSCKNQDIDDLNVAIQSYNYGGGYVGYVAGNGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVFGGSSPTTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVT"
     gene            complement(881..3085)
                     /locus_tag="KPK24_001559"
     CDS             complement(881..3085)
                     /locus_tag="KPK24_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2299239.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            complement(3085..5535)
                     /locus_tag="KPK24_001560"
     CDS             complement(3085..5535)
                     /locus_tag="KPK24_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2299240.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYEMIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(5513..5911)
                     /locus_tag="KPK24_001561"
     CDS             complement(5513..5911)
                     /locus_tag="KPK24_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2299241.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            complement(6029..6532)
                     /locus_tag="KPK24_001562"
     CDS             complement(6029..6532)
                     /locus_tag="KPK24_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2299242.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            complement(6550..7053)
                     /locus_tag="KPK24_001563"
     CDS             complement(6550..7053)
                     /locus_tag="KPK24_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2299243.1"
                     /translation="MQETRVLVETGRTNGEETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSADMQEEYVELLTVFENLEALYICR
                     NVITVYHDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENKRYLVTEN
                     SIFELPE"
     gene            complement(6972..7268)
                     /locus_tag="KPK24_001564"
     CDS             complement(6972..7268)
                     /locus_tag="KPK24_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2299244.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     KDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGYWWKREEQMAKKQHHTGLTYR"
     gene            complement(7367..8140)
                     /locus_tag="KPK24_001565"
     CDS             complement(7367..8140)
                     /locus_tag="KPK24_001565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299245.1"
                     /translation="MKGIKVLMISDEMRVDILHAVFINKNFIKDEENCNYEKYLLELV
                     NKSIYFREKSNFAEYVPPKSENHGECDCNSPNYQMDFKLLESTTRLHASKELTGQIQK
                     FCDGVIGKCPPRRPNTQMTVTRLFASLRDYDCESLHSCLTEKYEYGTIEFDIQTYVKL
                     LTFKKNLFFFFPYKFSFNTCYNFKYALDSIRIALEKDFRESNLFREKYYAEYDTFLAY
                     IYEDNLIISKFEQDGKLQMIDCIYLFKSQTYSKLYEYTW"
     gene            complement(8200..8421)
                     /locus_tag="KPK24_001566"
     CDS             complement(8200..8421)
                     /locus_tag="KPK24_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299246.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(8422..8556)
                     /locus_tag="KPK24_001567"
     CDS             complement(8422..8556)
                     /locus_tag="KPK24_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG2299247.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSISKEADYEMKQLEESEDN
                     "
     gene            complement(8569..9765)
                     /locus_tag="KPK24_001568"
     CDS             complement(8569..9765)
                     /locus_tag="KPK24_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2299248.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGIMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKYYQSGELIKHREDDR
                     EYMGSTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFIDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQAILSEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(9950..11344)
                     /locus_tag="KPK24_001569"
     CDS             complement(9950..11344)
                     /locus_tag="KPK24_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2299249.1"
                     /translation="MRMILNKGHRIRASDKNLVYRFSMGTLLFVFVAVILLLNTKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSVFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEQMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNLRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGFLAQARQ
                     DGTATCEAKGDGSD"
     gene            complement(11438..11875)
                     /locus_tag="KPK24_001570"
     CDS             complement(11438..11875)
                     /locus_tag="KPK24_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299250.1"
                     /translation="MCEECYSDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPN
                     RGLQRWNFPFKSLEIAKLYLRTADYTTKSSCGIYEIENSKGRVSYKIFAGNEDLHLFL
                     KKNKDKKCKQMTPVFNVGEYKEYPHTEVRKLTSDEIKQYMSER"
     gene            complement(11977..12360)
                     /locus_tag="KPK24_001571"
     CDS             complement(11977..12360)
                     /locus_tag="KPK24_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2299251.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNTGTSQNHTPQGQPKK"
     gene            complement(12376..12702)
                     /locus_tag="KPK24_001572"
     CDS             complement(12376..12702)
                     /locus_tag="KPK24_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2299252.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRKVIARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVEEK"
     gene            complement(12828..15872)
                     /locus_tag="KPK24_001573"
     CDS             complement(12828..15872)
                     /locus_tag="KPK24_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG2299253.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPSTAVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDKNGNTDVVEVKAGTVYIRELSAPAGYKVDTT
                     VYSLKVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAILPL
                     GTLSVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETSDTKPQGSATLKDTAFDVISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSAEWTSHK
                     HNTNAGKTSEDGVWFGTSEADDSKGALPYDTYIIEEMRCDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDSEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFIADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKEIEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKAEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDEGQTITFNEKPEVPEKLETPQTPEKPN
                     RPSDSPKTGDSTNVMAFLVMLLTSAGGLAGTYLYKRRTMKKS"
     gene            complement(16115..17479)
                     /gene="rlmD"
                     /locus_tag="KPK24_001574"
     CDS             complement(16115..17479)
                     /gene="rlmD"
                     /locus_tag="KPK24_001574"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG2299254.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHIETCVLLQ
                     RRTM"
     gene            complement(17654..19414)
                     /gene="pyk"
                     /locus_tag="KPK24_001575"
     CDS             complement(17654..19414)
                     /gene="pyk"
                     /locus_tag="KPK24_001575"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG2299255.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(19466..20425)
                     /gene="pfkA"
                     /locus_tag="KPK24_001576"
     CDS             complement(19466..20425)
                     /gene="pfkA"
                     /locus_tag="KPK24_001576"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG2299256.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(20634..24203)
                     /locus_tag="KPK24_001577"
     CDS             complement(20634..24203)
                     /locus_tag="KPK24_001577"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG2299257.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(24360..25307)
                     /gene="whiA"
                     /locus_tag="KPK24_001578"
     CDS             complement(24360..25307)
                     /gene="whiA"
                     /locus_tag="KPK24_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG2299258.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(25324..25749)
                     /locus_tag="KPK24_001579"
     CDS             complement(25324..25749)
                     /locus_tag="KPK24_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2299259.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(25818..26924)
                     /locus_tag="KPK24_001580"
     CDS             complement(25818..26924)
                     /locus_tag="KPK24_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG2299260.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(26936..27793)
                     /gene="rapZ"
                     /locus_tag="KPK24_001581"
     CDS             complement(26936..27793)
                     /gene="rapZ"
                     /locus_tag="KPK24_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG2299261.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(27824..28537)
                     /locus_tag="KPK24_001582"
     CDS             complement(27824..28537)
                     /locus_tag="KPK24_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG2299262.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(28541..29455)
                     /gene="murB"
                     /locus_tag="KPK24_001583"
     CDS             complement(28541..29455)
                     /gene="murB"
                     /locus_tag="KPK24_001583"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG2299263.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(29728..30498)
                     /locus_tag="KPK24_001584"
     CDS             complement(29728..30498)
                     /locus_tag="KPK24_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG2299264.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            30816..32297
                     /gene="cls"
                     /locus_tag="KPK24_001585"
     CDS             30816..32297
                     /gene="cls"
                     /locus_tag="KPK24_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG2299265.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSIFSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         9154 a         6852 c         4811 g        11574 t
ORIGIN      
        1 gttacatacg aaccagcgtt ataggtggaa tggaaaaata ccaaatcgcc agcctttgcc
       61 tgcgagagtg gaagatgttg ggtagcgtca tactgggctt gtgccgttct cggtaaggag
      121 ataccagctt ttccatagca ccattgcgtc agtcccgaac agtcaaagga agtggttgga
      181 gaagaaccac caaacacata tttccaccct tgatatttca acgcttcatt cattaccttt
      241 tgtgccagct ctcccgatac ctgcggaaca gttaaatact gattgactaa ttccacatag
      301 aacatatttc catagccata ccgccagcct ccgttcttcg caacggctat cgggttggta
      361 taggttactt tctttccacc cgatttctca cgggcgaagc tctctgcaag attaaaagtg
      421 tgtttctttc cgtttcctgc cacatagccc acatagccac cgccatagtt ataggactgt
      481 atcgctacat tcaaatcgtc gatatcttga tttttgcagg aagaaagcag ggacgcaaaa
      541 tacttacacc cctgcttgat tgagctttcc gtatctaagg aactaggcgg taaaccaaga
      601 ctttctgaac tctgcataac atcttctgcc gtaccgccac tttctacttg aatgatagcc
      661 agtagtacat tgacgtactc ggagataccg ttttctctgg cgtatttttc taccatagtc
      721 tgatgtttca aaacttctgc ggataagttc atacccgtaa tgccagaaga aaagttgctg
      781 ttttcgtcgt cgctgtccgc actaatcagc accccaaaga aaagcaccag agaaaagaga
      841 ataggaaaca gacttccaat gatagcgata tgtttcagtt tcattttttc ttctgtcctt
      901 tcttcgttac aagattacgg attttctctg tggtctgctg gttcttctgg acagtctggg
      961 tctgctgaac ctttgtccta cggtctttgg tgtaattctg gcgtgtttcc tttgacacgg
     1021 taactttttc tacattctgc ctatatgtcg attgggtcgc tttcccgtca ggagtactag
     1081 aagatagcgg acgttctttg acaccgcttg tcttaactgg ttcgcttgct ttcgtagtcg
     1141 gagttgtggc agggcgtttg acttcctgca ttttttccgc acccgtcttt gaaacggttg
     1201 aagctgtggc tggtcgctca tggggacggg tagctcccgt tgtcgctgat ccgtcagcct
     1261 ttcgctgtgc ctgccttgct tcttgtgcct tttgaagctc catacgcttg acagcgatac
     1321 ttttcttatg ctgttcctgc ttttccaagc gtcccgtctg tctggactgc tgttcctgca
     1381 ccataccacg cttgaaatca gacacgctgg actttgcctt ttcctttgcg gaatacaccg
     1441 cataagcggt ctgtgtgggc atatccttga tattctcttt gacagcgttt gccttgtctt
     1501 tcactttatt tttcgtatct aagacagcac ccacctttga gcctgcacgc tgtcccatac
     1561 tggaagtggt attgccccga ttttctttag aagccgtatt ttttctttcg gctctcttgc
     1621 cagcaacggc acttccagct accgcaccag ccacaccgcc cgaaataccg ccagcactta
     1681 cagcccttgc aatacggtgt tccatacgcc tagcccgatg tcgcatgaac agatacggtc
     1741 tgcggaatat cctgcgtccc atgctttgac tgtcgccagc gttgagagaa aacatactca
     1801 ttaagtcgcc cagcttcata tagataccag cgaaacatac tatctgcaag aacgccacca
     1861 tgaaaaacgg atagtccgtg gatatgttat aaaacatact ggaaatgcta aacgcaaccg
     1921 ttacaatgag cgttattcct gcccgtgtca ttatggtatt aaataccctc acgattgcct
     1981 gctttgccat gctttcatag ctcggtatca tggaaagtag aaagctgata ggtaaaaaca
     2041 tagcgaagat aataaaaagt atctggctga acaacatcat acccgtaagc aagaatacaa
     2101 atatcgtaat accaagatta aagaacaaga ggaagaacac catacctaaa cggtttacca
     2161 cctgcggtat cgtcagattg ttgttgtcgt tgtcctcgat ttctgttttc acgacttctt
     2221 ctctggtctt tccgtcctcg tcctctggac ttgccgatac gagagcttcg acacggtctg
     2281 tcccgatttc ctctgcgttg ctgtttccaa actgtaagag tagccacggc tgttgtactt
     2341 gaatagaaaa taggctgtcc cgtatcaagt ctacgctgtc cttgccctcg ctgtcagagt
     2401 tggggagcat gatttttgtt cccaaatcaa gcgaagcggt actgatgtct gatgaaaatt
     2461 catttatctt cttgatgtag tcgggagcgt aggcaataaa cgaagcggac agcacgaaca
     2521 ccaccacaaa gttgataaca gcgtgaagtg ccttgctggt ttcccgtttt atcagtcccg
     2581 tataggcaac atacattccc accactaaga taataagaag caggaaacca acatagaagc
     2641 ccgtagaaga aaagccgttc tcggtaacgc ctgcaagggt ctgtatgctt tttccgatac
     2701 tgtctgccat atcgttaata aaatcaagtt tataggcttc ctgcaccaca tagccagttg
     2761 cattgcttaa ataaaggctt atcgtccaga caaagttggt aatgcagtaa agcccgtact
     2821 gtaccgattt tccgataccg tccagccagt tccacggaag ccacgaccag ctattatcga
     2881 cgaaaaaatc aagctggtag ttggaaagcg gatattttga atatagattt tctgcgttta
     2941 tggtatcgtc cacaagcccc gtcgcatgag ccaccgtccc cagaagtgaa agcaggataa
     3001 gggaaagtgc caccacgaac agagccattt tgagaaagtg aagtatcttc ctttttgtga
     3061 acgcactctg tatcctttct ttcatcactc cacctcattt ctctgtacgg gcggtctagt
     3121 atcaaaggcg tgtagtaatt cttcaaagac tgggtgtatc tgcaccacac cgacacgccc
     3181 gtataagtcc tgcaataaac attgtccgtt ctccaaatca cgcaagcgtt tctggttgtt
     3241 ttcgtcgtcc ttgtcgatac cgaaaaattc taaggtctgc tttatctcgt tgatgtcagt
     3301 agaacgaaat gcaaacttca aaccgatatt gtttttcaga ctttcctttg acacgtcata
     3361 ggcagactgg gtaacgaaat aaacgcctgc ctgcatagct cgtccagcac gaaccagctt
     3421 atttgatagg gtttcgcctt gtgccacatt taagaacgcc cacgcttcgt ccaaatctac
     3481 aatcttaaaa atgcttctgt ccgaatggat aaaatcaagg gcaaaggtac taatcacaat
     3541 cagcatagac accgacaata attcaatggt cgtgtattcc tcaaaagtgg tatctttatc
     3601 tggcagtaca aggtctgcta cttgaatgat attgagctgg ttatcaaggc ttatagcatt
     3661 ttctaccgtc ccgtctgaaa acagcaggtg tgcaaagtcg tagtccgtga agctgtcgat
     3721 atggtctgcg atattgcgtg agatagcggt atcttctttt ctcaattcct caataacgtg
     3781 taacagtcct ctattttcac tctgggtaac agaacgcacc gcttttcgta acacggggaa
     3841 tttttcgccg tccctagagg aaatacccgt aaggaatgtt aagatgtcga ttgccagact
     3901 ttcagcgtct ttcacattct tcataatcac aaatgggtca agaagtcctg cattgtcctt
     3961 gtcgctggta aggtttacga tattgatttc atgggctatc tctgggagcg tttctttcca
     4021 gttgccacgc tcacttttag ggtctaacag aagtgcttga ccgccaaaca gcaccgcata
     4081 atatacaagt agattgttgc agaatgactt tccaccgcca agacttccca caaaagcaga
     4141 agcaagggca ttagtaactg tgccttttac tccctgcgaa gctaaagacg gttgcaggta
     4201 cacatttctt cccgtatcaa cggaatagcc catatagata cccgtgtttt cccctaactg
     4261 ctgggtcgca ccaaaaccaa gcccagccaa gaagtctgac tttacatact gcacatagtc
     4321 attgatatat cgcttgctgg caggtaaaaa ttcagaatga agtcccaaca tatcgccagc
     4381 aggacgcacc agttttacat tgaggtcgtc gtaaaagtcc ttgacttcat cacaacggcg
     4441 tttcagctcg tcaagatcgg gtgcagacac ccgtatgacg tagcttaact tatacatact
     4501 ttctttgctc tggtctaaat cggtttccag ctcgtccacg ctgtctaaag cgtccaccac
     4561 atttgagctg gtttcacttc ctgcttgata ggcgtgatta tccaaatctt tcaattcctt
     4621 tttcttgttg cggactgtcg ttaatgcctt acggttctcc acgatttcta cattcataga
     4681 agtatcaacg gggaatgtga attgctgttg ctggaaatag aagatttcag aggacggaaa
     4741 atccaactcc ccgacaatta cattgacggt aaaataggac acatagcttt ccttgtcctc
     4801 atgttccaat cgtaaatacc gctggctttc ctcaatcaca caccttgtcg gacggataag
     4861 gtcgtagtat tttatcagcg tttctttctt caatttcttc tttggtagct ggtactcata
     4921 atcttcatag gcgataccgt ccctgccgta aagatgttcc atgagatacc caaaatcatg
     4981 gatttccaag cgacgcacct taaagcgacg ggagatttta ttttccagta acttttccat
     5041 tttcatgtaa cggttgattt catcattcgg catggaaaca aaatcattca tcagcgtgtg
     5101 gttcacttca tgaagaaatt ccttgaacgt cagccatgcc gattttttga tgttcttcag
     5161 attgagctgt tcttccgtaa ccatgagctt aaagccaaga aaaaagcggt agtccacttg
     5221 attgtcccca atcattgata ctaacgcttc ggtctgttcg tctatttttt ggactgccac
     5281 ctcacgaagt ttgcctgtta ccagtttctt tgactgctcc tgcatacttc taatggagct
     5341 ttctgtggca atctgcaacg catgaatttt tccctcacgg gactgtgcga ttagctgtcg
     5401 aaaactgtca tgcacgataa atttctgctc tgcggataga aaagaatagt tgtacggtat
     5461 catctcatag taggcgaaca cctcattgtc cttgttccag acaaggttat tgtcaatata
     5521 ttttatcggg aacatagttc acactcctta ctgccgtgat tgtttcattc aatatctgct
     5581 tatgcagttt tacggctttt cctgcataag tgattttagg tcgcaaggca taggttatct
     5641 gtgatttcaa aaagctgtac ggcttctttc cgtcaaaggt tttctgcgac ataaaccaag
     5701 tgagagcaac gggaataccg aagtatttga gaaatgctcc ctcaatcatg gaaagtgggg
     5761 gcatatcccc aaacagaatg atgataaatt ccgtaatcac aaaccatgta atctgtgtaa
     5821 aggtaacggg aaagggtaag ttaaagtcat tgattgcata taagactttt tccacgttcc
     5881 agatacccgt gtagctttta atctttttca agttctttca gcccctttcg taagttcttc
     5941 aaagagaaaa ggacagccat tttcagactg tcccttaaag agaaatacgc tgtcagtagc
     6001 aacaatgctt gttgctgatc ttccagcgtt aatatggtat ctcacagata cctcggcttg
     6061 tttcaatgaa tcgaccaccc atatccaaat ctcgcccgta ggcttcatag tcaatatagt
     6121 tctgtaagtt agctggaatg tccccaagtg cgttcaattc gtctatgtag tagtaggcaa
     6181 catctgtcat ggtttcacaa tcgggataaa aataaatatc gtccttatgt tccacgactt
     6241 cttccagcgt cccatagtgg ctgataaatt cgtccagaca ctctacgata tagtcgggaa
     6301 gttcctctat catttcatac atttcattga gttcttcaat ggaaacatat tcccctatgt
     6361 cgataggaaa gttgtcggta tcatggacag cgtattcttc gtactgttca ttcaagccga
     6421 ttttttcttt tacatcttcc tcgtcaatag ggaaagtgaa ccacgcaccg actaaatatc
     6481 cctcattgta tctgccaaga ttagcaatat aaaccgccat atcatcaatc ataagcacca
     6541 cctcatttcc tattctggca gttcaaagat actgttttcc gttacaagat agcgtttgtt
     6601 ttcaagtaag tgagaagcgt aggcttcaaa atcgaaatag tatgcttgac agtcctcgga
     6661 taaatgtttg aatgtcgggt cgttcatcag cttttgcctt gcaacatcta tcatgctttt
     6721 acagtcgtga taaacggtaa tcacgttcct gcaaatataa agtgcttcta agttctcaaa
     6781 cacggtaaga agttctacat attcttcctg catatcggct gatagctggc ggtacataaa
     6841 atctaattcg ttaagctggt acacgctggt gtgttcgtgg acttcatcag cataaggcaa
     6901 caccttttcg ataatgcggt aatcttgact ttctgcaccg acacctaact tttcttcaaa
     6961 ctctgcaaca tctatcggta agtcaaacca gtatgatgtt gtttcttcgc catttgttct
     7021 tcccgtttcc accagtaccc ttgtttcctg cattgttcct cacgtccttt ctgttgtttc
     7081 atcacatctt ttaaggtctg gtatgacata ccaaacacat atttagaaaa atctttcaac
     7141 tgtttttctt catagtcggc agggttcagc cgtttcattt catgccacac cttacgcttg
     7201 taagagggca ggtcggaaat gtattcacag ccgacttttg cctgcatatc tttaaaaata
     7261 tcgttcattt catcactcca atctgaaatt taggtaagaa aaaagcagtc aatcctaaga
     7321 ctaactgctt tgtgtgtaag tatgaatttg tttaagtgta actgatttac catgtgtact
     7381 cgtaaagttt actgtatgtt tgacttttaa ataaatatat acaatcaatc atttgtaact
     7441 tgccgtcttg ttcaaattta cttattatca gattatcttc atagatatat gctaagaaag
     7501 tatcatactc tgcatagtat ttttctctaa aaagatttga ctctctaaaa tctttttcta
     7561 aagcaattct tatactgtct aaagcatatt taaagttata acaagtatta aaactaaact
     7621 tgtatggaaa aaagaaaaat aagtttttct tgaaagtaag caatttcaca taggtttgta
     7681 tatcaaattc aatcgttcca tactcatatt tctctgtaag acaagaatgt aaactttcac
     7741 aatcataatc ccgcaacgaa gcaaataacc tagttacagt catttgtgta tttggtcttc
     7801 tcggtggaca cttaccaatt acaccatcac agaatttctg tatttgacca gtaagttctt
     7861 ttgaagcatg aagtcttgtt gtgctttcaa gtaatttaaa gtccatttga taattaggtg
     7921 agttacaatc acattctcca tgattttcag atttaggtgg tacatattct gcaaaatttg
     7981 atttttctct aaaatatatt gatttattaa ctaattccaa caagtatttt tcataattac
     8041 aattttcttc atctttaata aaatttttgt ttatgaatac tgcatgtaaa atatccactc
     8101 tcatttcatc tgaaatcata agcactttta ttcctttcaa aaaataattt atcgtaccca
     8161 cattataaca ttctcatatt acagcaacaa taaaattcgt tatgctccaa taatcttatt
     8221 gaacagctct aacagtacat ctttcacacc gccagcgttg aatactaagc cgacagcaat
     8281 aagggcaact accaagaagc caataagttt tgaaaactca cgcttgaacc ctaagtagat
     8341 accgataacc acaatcgcca taagcactaa gctctgtgca tttgataaaa accattgata
     8401 caaattttgt ccgaaattca tttaattgtc ctcgctttct tctaattgtt tcatttcata
     8461 gtcagcttct ttgctgatac tcaaaataca catcaagact acgccgatac ccattcccat
     8521 agataccagc aggaagtctt ttactaattc ccacattttt tatcactcct aactttccac
     8581 caaatctttt gcaggggtcg tctgctgttt gattatcatt tcatgctttt cactcaagat
     8641 tgcctgcttt tcaatcgttt ccatgtagtc tgtaccgttt cccttatcaa tctttttcag
     8701 cattttcaag gtcggtgcta cctgtctttg tacccaacgc aatgtgcggt ctaaggtgta
     8761 aggttctggt tttgtcgtca gtttcagact ttgccgatta tcgccaataa accaagccca
     8821 gcggtcattg agtttccagt catttttccg cttgtctggt tcttcatcaa taaaccgcac
     8881 atactggttg atgatagaaa aagcggtctg ctctgcgtca tagtaggtca gcaaatctcg
     8941 tactgcatag taagcacgtt catttctaag ccgtatctca aaacggttga taatgtcggc
     9001 ttcttccaga ggtgtaccta atttgacata ctgctcatag tccttttcat agatacagaa
     9061 atacacatct gatttcagcg aaccaagata aagggtactc cccatatatt ctctgtcgtc
     9121 ctctctgtgc ttgataagct cgcccgactg gtaatactta tagcttctgg actttccgat
     9181 atattcccgt ttcctgcatt tctccgcaag ctctggaata tccaaaatgc ccgtatggtc
     9241 gttgatagca aggtcgatac gcttcataat gcctccgtct atgagtgcgt ccataagaaa
     9301 gtcataccag cttctttgct gtgccagcag gtaactttca aactgcctgc aaccccgacc
     9361 ctttaactct aaaaggacac ctttttcttc gtcagccgac gtatagataa agatgtcccc
     9421 taatgaataa tgctccgtat aactgtaatg tccgtaatct tcatggagca tataattgat
     9481 attcagtttt aatatgtctt tgatgatgtg ctgtatatcc agcgtgggaa aacgaatttt
     9541 cacataatca aacagcatgg taagcggtgc ttctggattg aaccttgcca gttctttatg
     9601 caaagtttcc agaagttctt ttgacggctt catttttccg ctttctatct tgtttagata
     9661 ttcccttgtg ataccagaag ctaccgccag cctgccttgt gagataccgt aagcaatccg
     9721 cttctcccgt aattctttta tccattgttc ttcattcaga accatacccc caatctgtca
     9781 agtgtgaagt tttttaaggc gacttcacag ccgatttttg ctaggaaacc atgccagaag
     9841 ccttatgttt cctatgtttt tttgtctatt gtctatttgc aaacgtaccc ctctgttaga
     9901 taccgagggg ttttacctgc tggcgtggct tacgccacac cagcaacggc tagtccgagc
     9961 cgtcgccttt cgcttcgcac gtcgccgtcc cgtcctgcct tgcttgtgca agaaaaccga
    10021 ttgtctgcaa aaaatcatgt cctttcggga ctaaaggcgt gtaaaattca cttatgacgc
    10081 ttgttcccac atcacaatag ccacgccctt tgatacgctt ttggaaaaac tgctttttca
    10141 catctgaacc aaacagcata ccgtaaccta actcgctgat acgccccaat cccacacgta
    10201 aattgaagtt atctctgata ccgtccgaga aatattttgc gtctggacgc tggcaggcaa
    10261 cgataaggaa ataacctgct tgtcgtccta acatgacgat tttctttaac tggctaagta
    10321 agcccacact ttcttttgtc cccagcattt caaaaaacgc cacatattca tcaaagataa
    10381 gaaagcaggg tggcagtccc agataggcgt aattttcgcc cgtcttatag ttcgggtgtc
    10441 gtttcatttg ctcgcttcgc tgtaccatgc cctcataaaa agcattgaca cagtctatca
    10501 tttcttcttt ggtgtgatac acgtttccca tgactgttcc caagtctgct aagtccgcat
    10561 ttttcgggtc taagatgtaa aggacagcgt tggtatgcag tagggcttca atgagcgtca
    10621 gaagaaaata tgttttaccg ccaccagtcc caccagcaat cagagcgtga gggagtgcgt
    10681 catattccca gacaagattt ttcatcagtc taagacagcc gttctctgcc cgtacttcat
    10741 caatggtaat gcggttcgct atcatatcat aaagcagggt atattcgata tagccgtcat
    10801 gcagggtctt gtcggtcagc tcacaataca agccactttc caatttatcc tctaaccgta
    10861 aaagctggtc ttgatatttc cccagcgtaa tttcacagcg gatatgaagc agtccctttt
    10921 ccatttgata ataaatcttt ggaaaccaga cgattttttc ccttgatctg ctttgcaggt
    10981 cagtaaaaaa aacgctgtct tgtacggtat cagcttcata ccacttgttt tccagtatca
    11041 ttcgtgccag cttttgacgg tgcagtagct ttttgaaact gtcgtaacag aagcggtaat
    11101 acagaaaagc gaccaatgca caaacaccag tcgctatcag tatggttatg aagttgtaaa
    11161 gggaaagcgt aaagccattg tctaataagc tgaaatgttc ccagtcggta cgcatgagct
    11221 gttttgtatt taatagcagg ataaccgcta cgaacacaaa caggagtgtc cccattgaaa
    11281 agcgatagac aaggttctta tcgctggctc tgatacggtg tcctttgttt aaaatcattc
    11341 tcataaatca atcactcctt tctgggtatg aaaaaagcag tcaatcgtat agactaactg
    11401 ctttgtgtgc atggatatga agttgtcaaa ctggaattta acgttcactc atatattgtt
    11461 ttatttcatc agaagtcaat tttcgtactt ctgtatgtgg atattcttta tactctccaa
    11521 cattaaacac aggtgtcatt tgtttgcatt tcttgtcttt attctttttc aagaatagat
    11581 gtaaatcctc attcccggcg aatattttat aagatactct gcctttactg ttctctatct
    11641 cataaatacc acatgaactt ttcgttgtat aatcggctgt tcgtaaataa agttttgcaa
    11701 tttccaatga cttaaaagga aaattccatc tttgtaatcc tctgtttggg tcatgttcaa
    11761 tacaaatgca acgtccacaa gttccacaaa tatattgagt gtggttttct aagcagtcca
    11821 agggatttag taatggtgtt attctatttt catcagagta acattcttca cacatttttc
    11881 ttttcgctcc ttatcaaatt ctaatttgtc gctttcttca tacccatatt ataacagttc
    11941 cgtatatcct ctacaacgaa aatctattca gcaagtctat tttttcggtt gtccctgcgg
    12001 tgtatggttc tgtgaagtgc ctgtgttctg attgccttta ttcttcagta caatatcttc
    12061 tgcctttaca taccagtcca catctgcacc cgtataggtt tttcgtgata ccgtatcggc
    12121 tacgggattg acaagctcca cgactgcatt gtaaggaaat tcccttaacg gtacttctgg
    12181 cggtacggac acggggataa ttccaccatg cagactgcac ttcaaatcat agatacgctt
    12241 tttaagctgg gtactcggtg tcccgtcctc attttgcaag aacacatcac gcaccgctgt
    12301 aaattttaat tctccaaatg ttttctcttt gtcaatgaca aatccatttg ataatctcat
    12361 aaattcttca ctcctttact tttcttcaac tgctacaata tcatcagcta ctaatacata
    12421 atcggtatgt cccacatccc cgattgcgta ccctctgccg tataacttcg gattgacaag
    12481 ttttactttc tgttcatact tgaaatgctt ttcgccagcc tgcacgggaa tttccaccac
    12541 aacattttcg cctttctgta cgtcagaata aaggttatag cttcgtctgg ctatgacctt
    12601 gcggttgttt ttatcccttt caaagatagg ctcgctttcg cctgcaaatt caagagttcc
    12661 aaaagactgt gccatatctg gcacgacata tttcatttcc atattgtttt acctcgtttc
    12721 tttctatatt tgataagttt atgattgtaa ttttttattc tggcttgtct gccgtttgac
    12781 agtttcggcg gagaacgtga tttttctttc atcataccgt taccgcctta tgattttttc
    12841 attgtacggc gtttgtaaag atatgttcca gccagtccac cagcagatgt aagcaacatc
    12901 acaagaaatg ccatgacatt tgtactgtcg cccgttttag gactgtcgct aggtctgttt
    12961 ggcttttctg gtgtctgcgg tgtttcgagt ttctctggga cttctggttt ttcattaaaa
    13021 gtaatcgtct gtccctcgtc ctcaatatcc ttatgctcgg taactttctt cggctcgtct
    13081 ggattgctta aatcatacaa ttcttcaaaa gttacaagct gtttgccgtc aagggaagta
    13141 gcgtcaaagg taaaggcaac ttccgctttc atagtttcgc tgtcagcggt aaaggtgtaa
    13201 tcactttcca cacgtttccc attgataaga agttctgcat tttcttcttt caacatctgc
    13261 cagcctacaa gtttgtactg tgtaccgact tccaagccct ctaaggttac ggtatcaatg
    13321 attgtaacgt cttttccagc ttcaatctct ttgttgccgt ctttgtcggt agcagtcgta
    13381 tgtatcttga tgatacgttc tgtaatcaat acagtttgtc cgtcgtcctc aatgtctttg
    13441 tgttccgcaa ctttcacggg ttcgtctgga ttgcttaaat cgtataattc ctcgaatgtt
    13501 accagatttt taccgccaag ctcggacgca ttgaatgtat aagaaatttc cactttcatt
    13561 tcttcatcat cagcgataaa agtatagtca ttttccacac gttttccgtc aatgataagc
    13621 tctgcatttt cttcctttaa catctgccag cctttgagct gatattttgt accttttgta
    13681 agcccgtcta atttgacagt atcgacgatt gttacctctt ttcctgcaag gatagtcttt
    13741 tcgctgtctt tgctggtcgc tgtggtatgg atagagattt ctttttcgta ttcatcagtc
    13801 aaagtcccta aatcaatcac aaggttattt cttgatacca cgatttcaaa aggtgggata
    13861 agttcaaagc ctttgttact atcgcaacgc atttcttcaa tgatgtaagt atcataaggc
    13921 aatgcacctt tgctgtcgtc tgcttcagaa gttccaaacc acacaccgtc ctcgctggtc
    13981 ttgcctgcgt tagtattgtg cttatgagaa gtccattcag cagaagtgga gaattgcccg
    14041 ttatcatcag taactacaac atgactttct cccgtcgtct tgcttgtgat cctaaaggga
    14101 acatcagcaa gacgcttgtg tgtgcctgca ccgattttca ctccctcaat atccccacgc
    14161 ttaatctgat tgtagataga gtgggcttcg tcggttaagt ccacgatttt tccattctct
    14221 gtgattgcaa agtcaatcgg cttcgcaccg tctgtcaagt aaccgtcggg agcttcactc
    14281 tcaacgatac ggaatttccc ataaggtaaa aggtcagcag aagtagaagc cacaccctca
    14341 atatctgtac gaattgtctt taccacttca tttttcttat atagcttgcc ctcaaccaaa
    14401 acagaattgt catttaagga aatgacgtca aaagcagtat ctttcaaagt ggcacttcct
    14461 tgtggtttgg tatcgctcgt ttctaaatct cttttctgaa ttttcacacc gccacggata
    14521 accttgtctg atacggaaaa ctggttactt cccgtcaata cagcaaggtc gccgtcctct
    14581 gtaatttgtg taaggtataa gccttttatc tgttcagact tatcgccagc ctgcatatat
    14641 gcaccgtcca gcaagtagcc gtttggagct tttgtttcct caacagatag tgttcctaaa
    14701 ggaagaatcg ctttgccgtc ctgcatatag aagctgtcgc cagatacctt gtaagcgtcc
    14761 gctaattttg tgatgtagtg agttgtcccg tcactgtctg tttcagcgat tgtctttgta
    14821 acccatgtac gagtagcttc ggcagggaga ttgtctttgt tatagaagcc agcataatac
    14881 ttccatgtaa actctgcacc ctctaaagaa gcgtttccct gcggattgtc tttctgcgtt
    14941 tccatatcaa tcttgaaaag ctcaatcaag gtgtccgtta cttttggtgt atctgatacg
    15001 ttcaaggtcg ctgtctttcc agcttcaact tttaaggaat acacggttgt atcaacttta
    15061 tatcctgctg gtgcggataa ttccctaata tagactgtac ctgcttttac ctctacaaca
    15121 tctgtatttc cgtttttatc agtcgtaagg gtggcaagct gtttcgtgca gtccttatca
    15181 gaaaagacac cataggtcgc accagcgatt gagtaattcc cgttaccgtc tgtaatgctg
    15241 gtattactgg aagtcttttg cagttttgca tttccgacat ttaacttcgc ccagaattgt
    15301 cccaattcct gcccctcgcc agagtagata taaccgccac attcatagcg tcctttattt
    15361 tctttcacaa aggctttagc accagcgaaa acttcgtcct gagttgcttt tggtatttca
    15421 tcataagaag ctcgcacgtt gtcgcattgc cagccaagat gtacgctcaa tctctgccag
    15481 acaacacatt gttccaacag atagacttgc ttgtagttca attccttgtg tgcttcgcca
    15541 tactgtttga catactctaa ggataacgcc acatctgaaa tctggtcggc actcatacgg
    15601 gtgcttgcgt cagctctggt cttgtagcca ttcttaaaat ctgtattgat gtctatacag
    15661 taagcgtcct cgccctcaac agtcaaatgt ccctcgttaa aggtagaacc aatcgaaccg
    15721 tcattcatta ctttttctac gataccgaca cgttcctctg attccgtcca gtattgcttg
    15781 ctttctgcat ggacggcggt actcggtaaa gcagtaacga cagtcgcaaa ggcaagtact
    15841 cccgtacaca atcgtttcaa tatctttttc ataattctaa atgctccttt cattttgggt
    15901 aaaaaaatag acgctcaata ttgaacgtct tacattgaaa agggatactt tttacaagta
    15961 cccctcaaat ctaatattta attgtttgtg ttcccacact ctatatcaac aactactgaa
    16021 acattgatat agttttttat ctctttattt ctgattttat cgccctcgct cgtaaagact
    16081 atatgcttaa aaaagtgcct aaaattaagg atttctacat agttcgtctt tgtagcaaca
    16141 cacaagtctc tatatgtgta gaatgaggga acatatctac aggttgtgcc tcaacacatt
    16201 taaatccgtg aacatttaga taatctaaat cccttgctaa agtagaagga ttacaagaaa
    16261 catagacaat tctttccgga ttcattgaaa caatagtctc taataccttt tcgtcacatc
    16321 cctttctagg tgggtctaca acgactacat tggcagtctt accctctcta tacatcttag
    16381 gtacaatttc ctctgccttt ccaacataaa attcagcatt atgtatattg tttaatttag
    16441 catttatctt agcatctttt atggcatcat ctataatttc tattccataa acctttttag
    16501 ccttttgtgc caaaaataaa gatattgtac ctatcccaca gtaaatatca aaaacagtat
    16561 cagtatcatt taaatttgca tattcaagag ccttatcata caatacttta gtttggacag
    16621 ggtttacttg gaaaaatgat aatggtgaaa tctcaaacac taagtctcct atataatcgt
    16681 ttatttttcc atctccatat ataactatat tttctcttcc tagaatagca ttcgtatctt
    16741 tttgatttac atttacaacc aaagtcttaa atccaggtat attttcctta agcactgaag
    16801 ctaattcttt aagatatgga agtttgtttc catttgctac taatacaacc attacttctt
    16861 tagttgtaaa acctattttt gttactaaat gcctaatcaa ccccgtgtga gtcttttcat
    16921 catatatgct taccttatag gcccttatat atgtttttat tattttaaca attttatcat
    16981 ttatctcatg ttgtattacg catttttctg tcgatattac atcatgagtc ttctttttat
    17041 agaagccaat aattggaata ccattattct tttgtattgg aaattgtgct ttatttctat
    17101 atctatatgg attatccatt cctatagtgt catgaacttc tacatcctct aacttaccta
    17161 ttctcgaaag tacttgttta acttcatttg ttttcatttc aagttgttta ttataatcta
    17221 agtcttgtat ctgacaccct ccacaatccc taagattgtc actacacact cttttcaccc
    17281 tgaatggaga cttttctatt atctcaacaa tatctcccac agcataattt ttctttactt
    17341 tattaatcct aactttaact ttatcatgta ttaatccgcc ctctacaaaa actgtaatac
    17401 cattgtactt tcctactcct actccgcctt gaccaatgtc tactatatct acaatataca
    17461 tctcgttttt cgaaagcata aatcaccctc caatcaatct attatattta aatctttaca
    17521 aacttttttc tttaaattta tattaaaata ttgctattaa ttaaactata ttttttacta
    17581 agctacaata ttttatttta ccaaaaaaaa aggagcttat aaagcccctt agtaaataaa
    17641 tttattattt ttcttataaa actctagttg aacctctata taccacgcct cttgatgcat
    17701 ccacagttac aacttctcca tcttttacta gatttgttat atcagctgca gacacaataa
    17761 ctggtatatc taaatttata cctacaatag ctgtatgtga tgtcattcca ccattctcag
    17821 ttactacagc tcctgctttt tctatataat tgttcatttc agcatcagta gcagtagaaa
    17881 caagtatgtc gccttcatta aagtctacac aagcatttcc attctttatt atacgaactt
    17941 taccttctac tgtttgagta cctacaccta taccttgcac gatttcttcg cttattacat
    18001 gaacctttat taaattagta gttccactta ctcctactgg tactccagca gttataacta
    18061 ctaattcccc attatctata taatcagctt gtctagctgc ctcaattgcc ttttctataa
    18121 cttcatctgt atttcctgca acagaagact taactggata tactccccat gtaagagcta
    18181 atttattcat agtcttttca ttatttgtag tagctattat tggagatttt ggtctgaatt
    18241 tagataccat tctagcagta tatcctgaag aagtagatgt tattatagct gatgcattta
    18301 aatctactgc tgttgtacaa gttgcatggc ttattgcatc tgttacagta acattgttag
    18361 taccattttc ctttagtaat ctatcataat ctaaagtctc ttctgttctt tttgctattg
    18421 tagccatcat ttttactgcc tctacaggat attttccagc agcagtttct ccagataaca
    18481 ttatcgcatc tgttccatca tatatagcat tggcaacgtc tgtaacttct gctcttgttg
    18541 gtcttggatt tcttatcata gaatcaagca tttgagtggc agtaacaact ggctttgcta
    18601 attcattaca tttttttatc atcatttttt gaactattgg catctcttca gttggtatct
    18661 caacacctaa gtcacctcta gccaccatta taccatcaga aacttttaat atttcatcta
    18721 ggttctctac accttcttgg ttttctattt tagatattat ttgtatatca gttgcattat
    18781 tgttctctaa tacttctctt attgctagta catcagatgc ctttcttaca aaagaagcag
    18841 ctatataatc tattccttga cttattccaa actctatatc acttatatct ttaggagtta
    18901 ttgctggtaa atttatctta acaccaggta catttactcc tttatgattt ttaactattc
    18961 ctgaattttc aacaacacaa actatatctt ctccatttat ttcttttact cttaagccaa
    19021 ctaaaccatc atctattagt atagaatctc ctgatttaac gtcttcagca agacctttat
    19081 agcttactgt acacatttct ttagtcccca taatatcttt catagttata gtgaactttt
    19141 gcccttcttc taaaaatacc tctgggtctt caaagttacc tgttctaatt tctggacctt
    19201 tagtatctaa taatatagca actggcttat ttaatttttc tctaactttc tttgccatat
    19261 caattctttc tttatgttct tcatgcgaac cgtgagaaaa gttaaatcta cacacattta
    19321 gaccattttg cataagttga gttaaaactt cctcactttg tgatgctgga cctagtgtac
    19381 aaacaatttt tgtcttcttt acattattta acatatttgc tcctcctgag atattattcc
    19441 acagatttat ttttaatact aaaacttata tagataatat ttttgccatc tcataagctt
    19501 ctttatcaaa tactttcttt tgagctaaag cttcacttat ttctaaatca attattttgt
    19561 tttcttttat tcctacaact ctagctgatt ttccatctaa taataattct actgctctta
    19621 ctccaagtct acttgctagt attctatcag atacagttgg gcttcctcct ctttggacat
    19681 gccccaatac agtgactctt aaatctgctc ctgactcttt tttaagttcc ttttctaaat
    19741 cactagcact tccgactcct tctgctaaaa cgattatact atgtctctta cctctttttt
    19801 gagtagtctt taatcttaga gcaacttcat ctactgtaat ttctacttct ggtactatta
    19861 tagtttccgc tcctcctgca agacctgcat ataaagctaa atctccacag tgtcttccca
    19921 taacttctac tatgttaact ctttcatgag atgatgatgt atctcttatc ttacctatag
    19981 catctataat agtattcatt gcagtatcaa atcctatagt ataatctgta tatgctaaat
    20041 cgttatctat agttcctggt atgcctatag ctgggaatcc taagtcacta agtttttggg
    20101 ctcctgcaaa tgaaccatct ccaccaatta caactaagca atcaatttta tactttttaa
    20161 gaatttttac tgcttttaat ctaccttctt ctgttttaaa ttcttcacat ctggaagatt
    20221 ttaatatagt tcctcccctg tgtataatat ctccaacaga agataagttc atttcagtta
    20281 aatcttcttc tagtaatccc ttatatcctc tattgattcc atatactttg catccataat
    20341 atattgctga tcttacaacc gctctaatcg cagcattcat tcctggagca tctcctccac
    20401 ttgttagtaa tcctatagtt ttcatttttt acctccttgg attttatgtc aattttttat
    20461 tataatcatt gttattgtat tctaatggac tttttttgtc cctatatcac tttttaatcc
    20521 catcaagact aaaaaaatta aatgtcctta aatgtccatt ttgtgatatt atatcacaaa
    20581 acggacaata tttttcatat tattataata tatttagatt tttatataaa aaatcaagct
    20641 tttactttta tgttatcttt aggaagtaac tcttctaagt tatttataaa ttcatcacat
    20701 atacaagtca atgccccggt cattttatat gctttacgtt taccatttat attttcatcc
    20761 atagggtata agtatatact gtcattacct ggatattgtt tggctatttc tacaatatta
    20821 ttagttgtat cagtatcaga catactatct attttcaaat acaatttttt accactttct
    20881 ggcttaatat tttctacatt tctagttctt cctgaattgt agtttctatc tgcaaagtca
    20941 gtatcatcat ttatatcttt tatctctcta gctataagtt ttgcattttc gccttctttt
    21001 atacttaatg ttccttttac atatattata ttatcttcat tcaaaatgac attatacttt
    21061 tgtaataatt gaggaaaaac tattatttct atagcaccat acaaatcttc taattcaata
    21121 aatgccataa tttcatttcg ttttgttgtt ttaatagttt tagttacaat cattccaccc
    21181 attataacat ctgtttcatt catttgtagg tatgactctt cttcctcttt taatgaattt
    21241 aattttccat tatccataga tgttttttct tgtagttctt tttcaaattg tgataaagga
    21301 tgtccactta catacattcc caagacttct ttttcaaggt taagtctctc tctttcttcg
    21361 tactcttgcc ttttaggcaa actgtatatt tgttgaaaat cactatcttc ctcactatct
    21421 ccaaaagcat caaataaaga aatttgacct tgaacatttt tctttctatc catagataca
    21481 ctttctaata aactttcata accagccatc agactagctc tattttcact tatattatca
    21541 aaagctccac attttattag actttctatt actctcttat ttgtatcttt ttggtctaat
    21601 cttttgacta aatctactaa atctacaaat aatccattag attctctttc agcgattata
    21661 ttgtttatta tatttacgcc aacatttttt acggctgcta gaccaaatct tatattgtcg
    21721 ccttcaactg aaaactttga aaaacttttg tttatatctg gaggtaatac atctatttct
    21781 agtgctttac attctcttat atactcaact accttatcag tatttcccat tacactggtt
    21841 atcaatgctg ccataaattc aactggataa tatgttttta gataagctgt ctcatatgct
    21901 aaaaccccat atgcagctgc atgtgattta ttaaatgcat atctagcgaa gtctatcata
    21961 tcatcaaata ttttattagc tatttcctct tctacaccat ttctgataca ccctgcaatt
    22021 tctatattac catcttcatc aaatttacca tgaataaaat attgtctttc ttcttccatc
    22081 acgtccatct ttttcttact catggctctt ctaactaggt cacttcgtcc gtaactataa
    22141 ccacctaaat ctctaacgac ctgcataacc tgctcttgat ataccaaaca accatatgta
    22201 acttccatta taggctttaa tttttcatgt agatatgtta catcttttgg gttgctttta
    22261 ttttttatat atgcaggtat agaatccatt ggacctggtc taaataggga tattcccgct
    22321 acaatatctt caaaattgtc tggttttagc tgtttcataa aacttctcat gccagcactc
    22381 tccaattgaa acacccctag tgtatttcct gatgaaagca tttcatatac tttcgggtcg
    22441 tcataatcca tctttgaaaa gtctattttt tctgtgtaat tttttataac tctatttttt
    22501 tctattaaat caagagtatc tcttataact gtaagagttc ttagcccaag aaaatccatc
    22561 tttaaaagcc caagttcttc aagtgttgtc atagtaaact gtgttgtgat tgcatcttga
    22621 tgtttgtaca aaggtacata ttcatctacg ggattttttg atataacaac tcctgctgca
    22681 tgagtagaag catgtcttag cattccctct atctgttttg atatatttat tatttctttt
    22741 gtttccgggt cttggtcata caattcttta agattaggat ttgtatctag ggccttatct
    22801 attgtcattc ctaaagcaaa tggtatttcc ttggctacct tgtcaacttt attgtagcct
    22861 atatccaata cccttccaac atctcttatt gcagcttttg cacccattgt tccaaaagtt
    22921 attatctggg ctacatggtc atcaccatat tttctcttta cataatctat aacttcttct
    22981 cttctctcat aacaaaaatc tatatctata tctggcatgg atatacgttc tggatttaaa
    23041 aaacgctcaa ataatagaga gtactttatt gggtctatat cagttatttt aagtgtatat
    23101 gctactatcg aaccagccgc actacctcta cctggtccta ctataatatt attttcttta
    23161 gaaaaattta taaaatccca aacaattaaa aaatactcta cataacccat tttttcaatt
    23221 acatctaatt cataatttaa tctctctagt atctcattac taggattatc atatctctct
    23281 tttaatccat taaaacacaa ctcccttaaa taagtttctg gtgtatatcc ttctggaaca
    23341 tcatacttag gaagatgtat agtattaaaa tcaaactcca cattacacat atcagctatt
    23401 ttcacagtat tgtcaatagc ctctaatgca tatggaaata attcttccat ttcttctctt
    23461 gattttaaat aaaactcatc actaccaaat ctcattctat ttgggtcatt taatgttttt
    23521 cccatttgaa tacacataag tacatcatgt attttcgaat cttctttatt tacatagtga
    23581 acatcattag tagcaactag tggtatacca gtctcttttg ataattttac taaaccagag
    23641 tttacttctt tttgctcagg taaattgtga tcttgaatct ccaaaaagaa attttcttct
    23701 ccaaatatgt ctctgtagtc aagagcaaat tgttttgctt tttcataatt tctattcatt
    23761 aaagcacgtg ctacatctcc tgcaaggcat gcagaaagtg ctataatccc tttgttatgt
    23821 tttttaagtt cttccatgtc aactctaggc ttatagtaaa aaccttctac gtatgaagtt
    23881 gaaactattt ttattaaatt tttatatcct tccatattct tagctaacag aactaaatgt
    23941 ccttgatatt tgtcatagtt tgggtcttta tctctcaatc cacgtgcagc tgtatagact
    24001 tcacacccta taattggctt tattccttct tttatagctg ttttgtagaa atctataaca
    24061 ccaaacatac aaccatggtc agttatagct attgagctca tgtttagttc cttagccctt
    24121 tttataagtt ttttcaccct cgaaaaccca tcaagtaaac tatactcagt atgtacatga
    24181 agatgagcaa aatctttgtt cattaaatca cctcctgctt tataatttta attttaccac
    24241 aaatatatgt cctaaaacaa aaacagagac ttttatctca aaataggaat taacttcaac
    24301 atataccatt atatgttata aattaatgct tattctgaga ttgtcccctc aatttattgc
    24361 tatgattttt ttttcaaatc tgttgcaatt tcttctattt ttctaagtct atgattgact
    24421 ccagattttc ctattggtgg ttctaacatc tctccaagtt ctttgagagt catatcctca
    24481 tactcgattc taatcttagc tatctcttgt aaattttctg gcaaactgct aattcctata
    24541 gtctcttgta tgagcttaat attttctact tgtcttactg ctgcatttac tgttttagaa
    24601 aggttagctg tctcacaatt tactatcctg tttacattat ttctcatttc ttttactatt
    24661 ttagtatttt gaagacttaa aagagcgtgg tgtcctccta taatactcaa caaatctgat
    24721 atttgttcac tctctttgat ataaacaacg tgattatttt ttctagccac aattttacta
    24781 ttaaaaccta aagagtttat aagttctttt agagaatctg caaactcctc attattagtt
    24841 acaaattcca tatgatagtt cttttctggg tcgcttatag aacctccacc aagaaaagct
    24901 cctcttatga aagctctctt acatccttca tgcttaatta agttctctga aattttgttt
    24961 atagtaaaaa atccttcttt atcttcaagt atacctaact tttttaataa catttctgaa
    25021 cccatttcgc ttgttaccat taatacataa ctgttatttc tctttagcat ttgattctta
    25081 tttactgaga ttgtagtatt gatattaaaa ttctttttta ataatttaaa tactttacgg
    25141 gctattgaat ttaactctgt tgttatcttt agatttagct tcttatatcc aacaatttgt
    25201 atacttcccc ctgatttaac tagagctgac aattcagcta tattacaaca ttcattccca
    25261 gatataactc ttgctagttc atttttggtt tcagttgaaa aagacatatc tatcacctat
    25321 taactaattt ttctttaacc ttttttttat ttcttgtaag gcaaccttta ttatctcctt
    25381 ttcatcatca tacctagcta agtctactac tctatctagg tggataaatt tagcatcaat
    25441 aaagccttcc tctttttgtg gaatagtatc catattcttt gcttgcatca aataccaaaa
    25501 aactgtttta tgaactgctc tattttcatc ccagttttct ttgaatgtat agtgtatttc
    25561 acctaaatat ttcaatatat ctgcttttac tccagtttct tcacttactt cacgtaatgc
    25621 tgcctcttgg ttattttccc cttcttctac ttttcccttg ggtaacaccc aatctccatt
    25681 gaacttcctt agtaaaagaa tcgtattgcc aaacaggact acaccaccag cactaacctc
    25741 ttctctcatc gatgttcact ccttaaaact aacataatga taaggatttc tacacaattt
    25801 tttaaaatcc ttttttacta acttttgttg taattatgat ttagggctaa ctcaataact
    25861 atgttagata tatactttgc atcatgtctt atataattat tttttatttc aattaaattc
    25921 ttctctacag tttttatacc catctctttt aatttatctt tttgcttttt atctaacaag
    25981 acttgctctg ctccatcttg tttatataag tcaagcattt cttctggcaa tatctcatta
    26041 tttgcgatta cataatctat taaattctca tcagtgtgtt ttactatagc attaacatgc
    26101 tctaatacat tgtacccttc tgtctcacct ggttgagtca ttatatttgc tatatatact
    26161 tttggagcta cagacgattt aatagcgtct acaatcccct ctacaagtaa attgggtatt
    26221 atacttgtat ataaacttcc aggacccata attataatgt cagcatcata aatagatgca
    26281 ataacatcct ttaatggttt tacatctttt ggatttaaaa aaattttatc aatagagctt
    26341 ttttgaattt ttgactcttc aggtatacta gattctccat ttattatatt gccattttct
    26401 aatttggcaa ctaaatttac atcttccaaa gttactggta aaactcttcc tgttatagca
    26461 aaaatttcac ttaatttata tacagctttt tcaaagtttc catacaaccc attcatagca
    26521 gccaaaaaca aatttccaaa actctgacct tttaaaagcc cttctgtaaa cctatattgc
    26581 ataacttcat tcattgttgg ctctatattt gcaagtgcta ataaacagtt tcttatgtct
    26641 cctggtggga gcattcctaa gtcttctctc aatacacctg aacctccacc atcatcagca
    26701 acagttacaa ttgcagttat attttgcgta gtgtgtttaa gacctcttaa aaataccgat
    26761 tgaccagtac ctccacctat cacaaccaca tttggttctc tattttcaac aatagacctt
    26821 tgaaatagtt ttactttttc attttttgct tttttatata taaataacga tacaattaat
    26881 gcaatccaac ctaaaattcc tacaataata agtaatttag ccatgtttgc ctccattatc
    26941 gcaacataga gtctctatgt ttctttacaa ctctatatcc cttattagat aaatcctccg
    27001 ctataagatt tgttatagta actgacctat gtcttccacc tgtgcatcca acaccaatta
    27061 ctagatgttg ttttccttcc tctatatatt gaggtactaa aaaatgaatc atatctaaaa
    27121 gtttcacata aaattcttca ctgattttag aattcataac ataatctcta acttctttat
    27181 catcaccagt tttagctctt aactcttcaa cataataggg atttggaaga aatcttacat
    27241 caaatactaa atctgcatca gcaagtatac catgtttaaa tccaaaagat actacagaaa
    27301 tagttaggtt tggattatct tcacctgaag aatatatttt ctttatttct tccttaaggt
    27361 ctttaggttt catgttagtt gtatcaatta tacatgtaga taactccttt aaaggttcca
    27421 ttatctttct ttccattttt ataccttctg gaatctgcat atcttttgcc aatggatgat
    27481 ttcttcttgt cattttatat ctctttaaca atacatcatc attacaatcc aaatatacca
    27541 tttcatattc ataattttct ttttctaaat aatttaagct ttcatgaagc gcttcaaaaa
    27601 actttctccc tcttatatct atccctagag caactttatc aatacttgaa tttggttgat
    27661 aacataattc tgcaaattta gttatcaaag caggaggtag attatctaca caataaaatc
    27721 ccatatcctc taaagctctc attgtctcac tttttccaga tcctgaaaga cctgtaacta
    27781 taacaaattt cattgctaac tcctcccttt tatcttatgt ttattatatt ataatgtttt
    27841 ctactaacga tatatccaga ttagagtcag atatagtctt catagctagt tcaaaatcac
    27901 caactctttc tggacaatgt gcatctgagc ctattataaa tttattttct agccctttta
    27961 ttttatttaa ttgatttaca ttcaaaaaag aatggctact atttatctca agtctagtat
    28021 ttgtcttttt tgctgtcttt gctacttctt ctatatacac atcgccttta tctcctggat
    28081 gagtaattat aaaaatatca ttattactta tcgcatttat tacagctttt gtattatatt
    28141 cttttgcttt ttcagtttta aaaatatagt tattacaatg atttaataag cttcttaatg
    28201 atgttggagt agaaccaaaa tgatatccag ccataatata atcaaaatct ctaataaatt
    28261 tatcatccat atctatattt cctttatcat ctaatatgtt acactccata cctaaaagta
    28321 tatttatatc agtgtactta tcatttaaat aatctacttc ttctctcatt ttgagaatat
    28381 cttttttctt tacaccatat gcaatatgtt tataactatg gtcagatatt cctattgttt
    28441 ttattccttt agaaatggct acctttacat tatcctcaat agtccctttg ccatgactat
    28501 ataatgtatg tgtatgataa tcagccagta ttttcatttg ttaatcctct ccaagtattt
    28561 tgacttcttc ttccaacata accccaaact tggcatatac agcacttttt ataacatata
    28621 ttaaatccaa aatatcttta gcacttgctt ctccttgatt gactacaaat ccacaatgct
    28681 tctcagatac ttgtgctcct cttaaagtaa gacctctcaa tccagtttct tctattaatt
    28741 tacctgcaaa atatcctgtt ggtctcttaa atgtacttcc tgcactagca aaatttaatg
    28801 gctgtttagt tattcttctc gtcgctaaat catccattaa actttttatt tcatcataat
    28861 ttccttcttg taattccaat tctgctgata aagctatata tccgtttttc gatattatac
    28921 tttttctata cccaaattcc atctgttcat ttgataattc aaatatattg ccttccatat
    28981 ccatcagtct tactgacttc actatgtcct tcatttcacc accatatgct ccagcattca
    29041 tggctaatgc tcctcccaat gtgccaggta ttcctgatgc aaactcaaac ccttttaatt
    29101 cttgcttttg gagagctttc cctaaaactg ataataaagc tcctgactga gcagtcatcc
    29161 tagtatcatt tatctcataa gaattgaaat tatcagctag ttctattacc actcctctta
    29221 ttccaccatc tttaattaaa atgtttgaac catttccaat tattaaatac ggaatagatt
    29281 cttcttttac caacttaagt acatttttta attgttcttc agttctaggt ctaactaata
    29341 tatctgctgg tcctcctacc ctaaatgaaa tatgtttttt cataggttca tcgactttta
    29401 tatcctcttt acttaatata tttaataaat ttatatatat atcttgatta ttcattgtat
    29461 ttcctcccaa attatactga actttagctg tcattattat ttaaattaca ttagtatata
    29521 gtattaacta tattcaataa tatatgattt tgcttatcat atggatttta tattttaagc
    29581 ttcttaatta caaactatat tcaataaata tataataaag taaaaaaaaa aaaataacaa
    29641 catataaaag aatataaata atttaaaaaa agcatcaata tcactgatat caatgcttta
    29701 aaaggcaatt taattcgtat tatttatcta actatttttg acatagcaaa gataataaat
    29761 ccctccaact actatggaac ctccaacgat atttccaagc gttacaaata ccaaattagt
    29821 tataaattga accatagtta tatttgctcc taatgccatt cccattggta taaaaaacat
    29881 gtttgcaaca ctatgttgga atccacataa tacaaatagc attattactg tccaacatga
    29941 agccaatttt gacattatat cttgagatgc tgtagccatc catacagcta aaacgactag
    30001 aatattacaa agtattgctt ttaaaaacat tgatgctata gttgaattaa ccttagcttc
    30061 tgccacagct atagcttttg tagctgcatc tcctgtcaat gtagaagaat aaaacacaag
    30121 tgcaactagc aaaattgaac ctactaaatt tgcaaaataa accagtcccc agtttcttaa
    30181 catttttcct aatgttattt ttttgtccat aagtgcaata gccattaagg tgtttccagt
    30241 gaataattct gctcctgcca ctactacaag cataagacct actggaaaga ccatagcact
    30301 taaaaagcta gctacccctg ggtctatctt gccaaaagtt tgactaacta tcatactccc
    30361 caatgcccca aaacctataa atgctcctcc aaataaacct aatagaattg cgttttgtgt
    30421 cgaaatattt gcttttttaa tgcctgcact tatagttgct tgtgcagttt ccccaggtgt
    30481 caagaatcct ttactcataa atccttcccc cttaaaaatt atactttccc ttgtcttttt
    30541 atggcaataa ttatataata acaatatgat atataataaa ttttattaac ataaaaaata
    30601 tactatatac ttatatatac ttgtctcata taattatact atttattata taacaacttt
    30661 gttaatttcg aaataattta tattgaattt gaaaaattta ccttttgatt atttatttta
    30721 ctttttaatt attgaggtat tatatataat agatatatgt ttgtgttatt atatataatt
    30781 acttatttta ataaatattt gaaggaggga attttatgtt tgatatatca tttttcgaaa
    30841 tgtcaatata tgaaatagca gttactacaa tatatataat taatttcatg gttatactaa
    30901 accttatctt tagagaaaaa agaaacataa atacaacttt aacatggctt cttatattag
    30961 tattgattcc tgctcttgga ttcatacttt atatagcttt tggcagaaat atttcaaaaa
    31021 acaatatgtt tagattgaaa gaaaaggatg ataagataat caaaagtaat atactagata
    31081 ctcaggttaa actccaatct acatctgaaa ttgattcaga tatacatcaa cataaggata
    31141 tgatttatgc attggctaat tcaaataatg ctcattatac caataataac gatgtgtgga
    31201 tttatgctga atctagtcaa ttttttaaca gtcttcttga agaacttaaa aaagctaaaa
    31261 aatatataaa catacaattt tacattttca aagatgacaa aatcggaact gaaataatag
    31321 acattcttat agacaaagct aaagaaggtg ttgaagtaag acttttattt gatgcagttg
    31381 gtggtagaac actaaaaaat agtacacttt ccagacttaa agaaagtggt gttaaagttg
    31441 gaagcttctt cccatcattc ttgaaaattg taaactttaa cttaaactat agaaatcacc
    31501 gaaaaattgt tgtcattgat ggtaaggtag gatttgtagg tggatataat gttggtgatg
    31561 aatatttagg tagaaatcca aaatttggtc tatggagaga tacacatact aagcttactg
    31621 gtgattgcgt aattgaccta aatatgagat tcatccttga ttggagatat acaactaaag
    31681 aagacttaga tttagaaaag tactttatag aagaagatac aaattctaat catccttctg
    31741 aaaatattgg gattcagata gtatcaagtg gtccagatat atcagaactt gatgagatta
    31801 aatatggtta ccttaaaatg attcagaaag ctagaaagta tatatacata cagagtcctt
    31861 atttaatact tgactcaact tttatagata ctttaaaaat agcttgttta tctggtgttg
    31921 atgtgagagt tatgattcct tctaaacctg accatccctt tgtatactgg gcatcatatt
    31981 catatgctgg agaactttta aagtttggag ctaaaatcta tacttatggt caaaatgcat
    32041 ttttacatgc taagacaata gttatagatg actctatatt ctctatagga acagctaata
    32101 tggatatcag aagttttgag cttaatttcg aagttaatgc atttatgtac tcttcaaaaa
    32161 aagcattgga acaaagaatt atatttgaaa atgatatttt aaattctaag gaaataacat
    32221 tagatatata taattctagg tctacttata taaaaatcaa ggaatcaatt tcaagactat
    32281 tatcagcagt tttataaatc ttttatttta taaaaatttt acattttggt ttatttttta
    32341 aagtttttta tatgtattat ttttttttta ttgaatttag ctcatttttt t
//
LOCUS       DAEMGG010000036        31407 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEMGG010000036 DAEMGG010000000
VERSION     DAEMGG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.036"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            325..2220
                     /locus_tag="KPK24_001586"
     CDS             325..2220
                     /locus_tag="KPK24_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG2299266.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            2682..4085
                     /locus_tag="KPK24_001587"
     CDS             2682..4085
                     /locus_tag="KPK24_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG2299267.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     regulatory      4257..4342
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4466..10429
                     /locus_tag="KPK24_001588"
     CDS             4466..10429
                     /locus_tag="KPK24_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBG2299268.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDGLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQEISKGNSDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     gene            10639..12519
                     /locus_tag="KPK24_001589"
     CDS             10639..12519
                     /locus_tag="KPK24_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG2299269.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVIGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVGYENINSDEGLVFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTIKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISKNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            12886..14487
                     /locus_tag="KPK24_001590"
     CDS             12886..14487
                     /locus_tag="KPK24_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG2299270.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSVSGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNRQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            14706..16295
                     /locus_tag="KPK24_001591"
     CDS             14706..16295
                     /locus_tag="KPK24_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBG2299271.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKA
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGAPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNINTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVIDGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            16508..18688
                     /gene="slpA"
                     /locus_tag="KPK24_001592"
     CDS             16508..18688
                     /gene="slpA"
                     /locus_tag="KPK24_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG2299272.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPVFAAQTDYQNTYTVVQSKYEKAL
                     KDIQKGITDKKITEVGISYEGKPVTTITVANMDTTGTTSTKEELASALLKTTVNDKLD
                     NLGDGDYVDFDITYVGAADALTAGDLNAFKAALAADTTLKIPVASTTKFGAVVLGTGD
                     TKLDPASSAVNVSTAIEANIVGNTLTVSKKASDATKIGKEDEDNSTATDVTFKDDAKI
                     SVSVGDPKIDLAKSFAFDDTTGKLDGIVEKENTATSHAYVRVINAKEQTIDLDASSYK
                     SAEDLAKAYAFDVNELKTLYTEIEAYQKDSNNKTDKVQIVDGKYQTILYAEGKRLTTK
                     SATQASKLADENSPLKLVIKADKLKDLKDYVEDLRNANNGYSNTVTVAGDDRIETAIE
                     LSSKYYNNSDEDNAITEDAVNNVVLVGSQAIVDGLVASPLASEKKAPLLLTSKDKLDS
                     NVKSEIKRVMNLKTTTGINNSKKVYLAGGVNSISKEVENELKDMGLKVTRLSGDDRYA
                     TSLEIADEIGLDDDKAFVVGGTGLADAMSIAPVASQLNQKGDATPIVVVDGKAKELSS
                     AAEDFLDDSQVDIIGGKNSVSKDMEDAIDDATGKSPNRVSGDDRQETNAEVLKESDYF
                     PDGAVNYFVAKDGSTKEDQLVDALAAAPVAANFGRTYNIKDNDSSGTVSPAPIILATD
                     SLSSDQNVAISKALPSGKSGNNLVQVGQGIANSVINKIKDLLDM"
     gene            18936..21281
                     /gene="secA"
                     /locus_tag="KPK24_001593"
     CDS             18936..21281
                     /gene="secA"
                     /locus_tag="KPK24_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG2299273.1"
                     /translation="MSVLDTILDKADEQEIKKLNLIVDKIEALEEEIQNLSDDELKEM
                     TNVFRNRLNNGETLDDILMEAFAVVREVSKRKLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYESLGMTVGVIISNQD
                     PNIRKQQYKCDITYGTNSEFGFDYLRDNMVPDLSYKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGDGDEDLKLYELANSFIKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEIISKAGKLDAITIATNMAGRGTDISLGAGDKEEEQEVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            21696..23567
                     /locus_tag="KPK24_001594"
     CDS             21696..23567
                     /locus_tag="KPK24_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBG2299274.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEAIEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGINLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFIIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNVVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKDT
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            23674..24381
                     /locus_tag="KPK24_001595"
     CDS             23674..24381
                     /locus_tag="KPK24_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG2299275.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            24414..26249
                     /locus_tag="KPK24_001596"
     CDS             24414..26249
                     /locus_tag="KPK24_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG2299276.1"
                     /translation="MKISKKIVALLTITFLTITLYGNTSNASTKYTLTGSGRRETAIK
                     ISQAGWKKSEKAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENINFERISGNSRYDTSLKLAEKLNKEKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSDNGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRNKNDLIDSLAVGV
                     LAAKNSSPIVLAGNKLDTVQKDVLNTKIIDKVTQIGGLGNENVVEDILDIQEETKYTV
                     ETIDELNVVIKKADANDIIKFVPEKDRSINSSFKIETDKAIEVELKGVYKQSITINMP
                     NGKISNLGEINGTTEIKNIKNNSFINKGSIFDLDISSNNECKIENESSGEIWLITIFQ
                     NAKGVYIVNDGDITKISNNSSDVIIKNSGEIDTVTGSKEPAISGKKPRINDTKVDDTL
                     ARGLYVKADSCYNPEKDYVMVTILSEPKSSDYSIYYRVVENKPSAMYVGNKINPADWD
                     IVTKYNKEFIEKAKDGSYIEVVEVNSLTNKVSRWGKSNVTNDGVR"
     gene            complement(26292..26687)
                     /locus_tag="KPK24_001597"
     CDS             complement(26292..26687)
                     /locus_tag="KPK24_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG2299277.1"
                     /translation="MIFKKHKETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIKFLFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKN"
     gene            26915..29326
                     /locus_tag="KPK24_001598"
     CDS             26915..29326
                     /locus_tag="KPK24_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBG2299278.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTYKRAPLNHAVAIVGWDDNYSKD
                     NFASDAKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVANGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            29623..31200
                     /locus_tag="KPK24_001599"
     CDS             29623..31200
                     /locus_tag="KPK24_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG2299279.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGAAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERLSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPNENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKFGFLKKFLS"
BASE COUNT        13210 a         3512 c         5759 g         8926 t
ORIGIN      
        1 gatattaatt tagtgatata tatttaagta ataagagtaa agcaaaaatt agtgcatagt
       61 agtatataaa aatggagttt gaatactttt gttagtatat gattgtttag tagaaaatca
      121 ctataaaata ggatttctat ctttgagcat aattatagag agcctaagta gggggaaggg
      181 tttcaagaag atattattag tgtttgtgtt agtaaattat gtaaacacat aaaatatatt
      241 gaaaaataat attatatata atattattta tgtgtgggaa atacgaatat aaaaaataaa
      301 aaataatatt cggaggacat aaatatgtta agcaacaaaa agagaagtat ggcaatagta
      361 atggcaggtg ctacagttat gagtgcagca gcaccaatat tcgcagacaa taccgtgaca
      421 gaaaatgttg ataaaaatta cacagtaagt gcgaaagatt ctgctaaact aatcgaagag
      481 gttagaaaag cattagaagt taagtttgaa gatacaaaag caggagcaaa tgtaaatgat
      541 agagtttacg atataaaagt agacaatgta aacttaacta atgcaactca attacaaaat
      601 aaaataaact ctttaacaga agggcaaagt ttaaaagtta ctattcaaga taaagggcat
      661 caagtgttag gtggaaaagt agttgactat aaaattgaaa actacaaaac tgctcaagaa
      721 atagtagatg cagttaatgc ttacaatgca actttagcag aagattctga taacaagtta
      781 acagcaacta taaaatctac aaatacagtt gaagtaaaaa gagcaaaaga ttcagcaaat
      841 gttattacat taaatgtagg agaccaacat ttagatttct caaaagtgat aacatcagaa
      901 gaaggtactt ttgaaggtta tgaaaagagt tatagtgaca tagattcaaa agaattacat
      961 acgataacag ttaaaaacgc agatttacaa gacatatcag cagaagaatt atttgatgga
     1021 attagattaa ctacattagg aagagaaata gttaataaag ttaaaaatgg atatgcttta
     1081 acatttgaaa atgaagcaat ccttactcaa gaacaagaag atagtgatga taaagataaa
     1141 ccagaaaaat caagctttga tatagtttta agcaaggcaa atgaaaagcc agaaacaata
     1201 agtgtttcaa gtaaaaacca taagttagtt agagacttac acaaagtatt aactgatgta
     1261 aaagatggaa aagaattaaa agtagaagtt ttatctggtg attcaagatt tacaacagca
     1321 gtagaagtta gtaaagagag atttaaagat ggagaagcag aagctataat tttagttgga
     1381 gaagatgcta tagttgatgg attagcatca gcaccacttg cctctcaaaa aaatgcacca
     1441 atattattat ctaaaaaaga ttcactacca tcagaaatag aagctgaaat attaagagta
     1501 cttggaagta atctatcttc taagaaaata tatatagtag gtggagaatc taaagtatca
     1561 aaagaaactg aagaaaaact ttctaaatta ggtgtaagta aagttgagag agtttctgga
     1621 gaagatagat ttgaaacttc tttagaaata gcaaaacaat taaaagatac atttaagact
     1681 gcttttgtag taggtggaaa tggagaagct gatgctatga gtatatcagc tagagctgct
     1741 caatttggtg ctccaataat agttacaggt aacgaattag atgcaaatgc tgaaaaatta
     1801 ttaaaaggaa aagaattaga aatagtgggt ggagaaaatt ctgtatcaaa agaagttgaa
     1861 gacaaattag ttgatataga tttaaataat aaagttgaaa gattagctgg agaaaataga
     1921 aaagatacta atgctaaagt aatcaataaa tactatgcag gtgcaactaa agcatatgta
     1981 gcaaaagatg gttatgtagg tggaaatgga caattagttg atgcacttac agcagcacca
     2041 cttgcagcta gttcaaaggc tccaatagta ttaactacag aagaactttc taaatcacaa
     2101 gaagaagtag ttgagttaag acttaagaat gctactaagt tagtacaaat aggtgaagga
     2161 atagctaaaa acgctattga aaaaatagca gaaaaaataa acttatttac taagaactaa
     2221 taagatatag gaattaatat taaaagttta tttagataat atatgattat acaatgcgtt
     2281 aatatgtaag acatagtgta gatattataa aatatacaaa taacaagata aaataataaa
     2341 aataaggtgt ctcatttaga attagattga gataccttat tttatttttt gattataatg
     2401 ttgagaaatt catattgcaa aaattattat aaaaagatac ttcttataat gattaaacat
     2461 attactaaca taaattattt tgaacttata acgtaattaa ataaaataaa ttataatgtt
     2521 ttgaatttat agagaataaa aactttaggt atattgtttg agttgtatgg ataactatgg
     2581 tatcatatat aaaatatggt aacaaaattg taacttttga ttaaaaataa gtatgagtac
     2641 taataaaaaa tatgaaaagt acattggggg tagaaaataa tatgaagaat agtaaaaaga
     2701 tattagctat aggacttaca ctatttttag taatggtaaa tactcctatg gtaaatgcgt
     2761 tgacatcagt tgagcaaata aaagggaatg acagatatga gacagcagca aaaatagcag
     2821 ataagcaaaa ctacaataca gcaatactaa tcaactcaga taatagctta gcagatggtc
     2881 taagtgcaag tggtttagct ggagccttaa atgcgcctat tttgatgaca aaacaaaacc
     2941 agattccaaa cacaactatg gaaagattaa acaaagcaaa gactgtgtat ataataggtt
     3001 cagaatcaac aataagtaaa aatgttgaga atcagttact atccaaaaag aaggttgtac
     3061 aaagaatatt tggtgaaaat agatttgata caagtataaa aatagctgaa aaaatcaaag
     3121 aaattaagcc aatagataaa gtaattatag ccaatggatt tacaggagaa gcagatgcga
     3181 taagtgcatc accagtagct gctagagatg gagtacctat aatacttaca gatggaaata
     3241 gtgtgggatt tgacacgaca ggcttaaaga gttatgcgct tggttctagt gaaataataa
     3301 gcgacgaatt agttaagagc acaaattcta ttagattggg tggcacagat agatttgaaa
     3361 ctaataagat agttatacaa gagttttata aaaatagtaa agaattttat ctaagtaaag
     3421 gcttacaatt aacagacgct ttggctgcct ctacaatagc aaaaaatgct cctgtagttt
     3481 tagttgaaaa tggcagtaac aagagtattt taagtggtgc agacaaattg actgtactag
     3541 gaggcataaa tcagaatgtt atcaagcaat gtataaatca agcttcacca aatcaacaag
     3601 gtttatatta caatccaaat gatagagcat ttaaagagag aataaagggt aaagtatatg
     3661 ctcttacaaa gcagtataga aaagagaatg gagtaagagc gttatctgta gcaagcagat
     3721 tagagggtct agcaaatgat tggtctaatt taatggcaaa taagaagact ttatctcata
     3781 cgataaatgg aaaaaattca tattctactt ttttgaaata cttagattgg agtgagatta
     3841 aacctggata tatagcagtt caaggtgaaa atataattaa atataaaatt ccagataaac
     3901 ctgtatatac aaatagagat gcagatgata taggaaactt tatttttaat gagtggaaga
     3961 caaatccaga agaaggaact aatatgttgc ataaagggta tgaaataatg ggatttggta
     4021 tagctataac aggagataag aatctatatg ctacacatga attttatgga agatataaag
     4081 aataagaaaa tatacacaaa attatgcttg taaggaagct atataaataa ttaaattata
     4141 atggaaaata gcatttatat taataaatta atataaatgt tattttttta taatttaatc
     4201 taaaaaaatt atattaaaaa gtttaaatat agtataaaat gggtaagaaa gtagtgaaaa
     4261 ttaaatggca aaattaggga aatctaatga cgcaaaacta tagggattaa agcttttaaa
     4321 gccatatcag ccagttgcta aaaagagtaa agtcttttta ttttataatt aagattctct
     4381 ttgagtgtct ttttttattg tctgaaactt atagtattta aattcatgaa aaattagata
     4441 tttattaagg gaggattgtt atttcatgaa aaaggcaata tcttgtgtac tagcagtatc
     4501 tatgtgtagt gcaccactaa acgtttttgc agaacctata ttagagggga agctaagggc
     4561 agttgaaaaa tcagttacag aaaagttaag ggggagtctt gaagtagatt taaatttttc
     4621 actgccaata aaaaatagcg agtcagaaag tatgactaat atgcagttac gattaaaaga
     4681 tgatagtaac aatagtggaa taataaagct tggagaaaaa gattcaggaa cagtaaatgt
     4741 tgggacagat aatatagaat atactataaa aaaattatct gcaagtagaa gtgaaataaa
     4801 aaataaagat gagaatatat catattataa tattgtattt aataatttac ctgttggaaa
     4861 atacaatgta gaagtctctg gagatggatt taagaacaaa gaaattaaag acattgatat
     4921 atcaagttat tcacaaagag tcttattaag caatagagca agtgtagata ataaaaataa
     4981 acctttaaat tatgaattgt tcttaatggg tgatgtaaat ggagacggta aagtagataa
     5041 gagtgattat aataaggttt tagagaatat agattcaaat aaaagagaat ttgatttaaa
     5101 tagagatgga aaggtagata ttgttgactt agattatgta cagaaaaatt taggacaaag
     5161 tgaagatgca aaaagcctag aaagtatagt atctaccaat cctatagtag atacaagtaa
     5221 ggttgaatta aaaggaaata gtgatgtaga tattaatggt aatgtagaaa atttatttag
     5281 tggagaaaat aatggagtta caatatcttc aaaaaatgat gaaatatctg aggaaaaacc
     5341 agcagtaatg gatatagctt tatcagaagc acttaatatg gaagtcatca atataaaagc
     5401 tccagaaggt actgctccag caaaaggaat agttgaggtt actgacaaag atggtaatgt
     5461 tcaacagatt ccttatgaaa acaaagaata tatgaaaaac ttaaaagatg aggagcaaca
     5521 agaggaagta aataacgaac aagaagaagt aaatcaagaa gaagtaaata ataaagatga
     5581 aaatataaac cagactgata ataactcaaa taataaagat gaaaataatg ataaacaaca
     5641 ggaaaatagc gataaacaag aagcaaactc taataccaat caagataaaa ataatgataa
     5701 agaaaattca tcaatggata aacctaattc agatgaagat actccaaaag aagagggtaa
     5761 tatcagtaaa gatgataact ctacaacatc tacatatagt aggtcagcat tagaaacata
     5821 tgcagatgtt caaaatagtg atatagtaat aaatcttggt aaacaagtag cagtaaaaaa
     5881 gataactata aaaataacag ctacaacagc aaacgataga aatcttgcag aaatatcaaa
     5941 agttgaattt ttaaacaatg tatataaaga aataccaaag ccagagatga acatacctaa
     6001 aataaataaa atatcgacta gcactgctgt tggaaatgaa aaagtatctt tttattggaa
     6061 taatgaagta aatgtatcag catacgaact tatactagac aaaattgata gtaagggaaa
     6121 agtattatct actaaaaaat tacaaactag taaaaataca atagaagtta gagatataga
     6181 agcatatgga ctatatagag ttagcataca atctttaaat ggagattggt ctagtggtta
     6241 taaagatgca acaccagaaa acttagacgg agtaccagaa aatgtagatt caaactataa
     6301 acctatacaa tttagcccag atagtatatt agaatttcaa gttgttccag acacaaaacc
     6361 agaaccacca gaaggtataa atgttaaagg taaatttaaa tctcttgatg taagctggaa
     6421 aaatcataaa caagctaaag attttgactt gtactataaa gaaaaaggct ctaatgggtc
     6481 ttggataaaa tacaatgata agcttattcg tgggacaagt tcaaagatag aaggtcttaa
     6541 ggataataca acatatgaag tcagaatgac agctactaac cacttgggaa ctagtggaat
     6601 gtctaaaaca tatataggta caactgaaag catggatgca ccaacaacac ctaactatag
     6661 attaattaat actcctaaag aaaatggtgg atttggaata ccaacaaatc acattgtaga
     6721 tgttgtttat ccagatggat atgacaaaag tgaatatagt gataaaaatc catttgataa
     6781 attcaatgta gtagatgggg actttacaac acattggaca ttcccaacat ataatgcagc
     6841 tgagggaacg aatagaggac caataataac ttttgataaa tcttatacta tggatacttt
     6901 tatggctaca cctcgttttg atgtttctga taagacaata aatgactttg aggtacgata
     6961 ttgggatgaa aatggtcaat tacacaatct gggggatgtt ccttttgaaa caaagacaga
     7021 ccctgtaaat aataataagt atgtaatggt tagattaaaa gaaccaataa ctgctaccaa
     7081 gatacaagta agtgtttgtt catcctattc atataagtct aatatatcag aacttaagtt
     7141 ttatgagtat gacagtatag aagatgatgt aagaaatcta tttatggatg gcctacaagt
     7201 tgaattaaaa gaagatgtaa ctcaagaaaa aataactgaa ttaaaagata gattaaatac
     7261 tccagataaa gctagtggag aatatcatcc atataagaca gttattgaga gagaattaaa
     7321 attagctcaa gacttatata atgatagagc cacacttact gatgtaacta ctgtaaatca
     7381 agaaataaat aaattaggaa aatataaaga taaaaacgga cagtatactg taaattccaa
     7441 taatttaggt atgcaaaatg actggcaggc attaggagta gctgctagag caggagatga
     7501 aatcacagta tatgttggaa gtaaaagtgg aaaaactcct aagttgatat atacacagta
     7561 ctatggagaa tctggtgcat ataagagtgg agaaataaat cttaacactg gaaaaaatgt
     7621 aattcaatta agcaaacttc atagtttaga tatagaatgt ggaggaagtt tatacataag
     7681 atacgctgaa gatactccaa gtggaggaga tataaaagta cgtgtagcag gagctactaa
     7741 gataccacat cttaatctaa atggaacaat aaatgataag agtcctgcgg gagtttctga
     7801 atctaagaaa aagataaaag catatataga agaattagct aaatataaag atgctgtaaa
     7861 aggagaacca tattatccag gaacaaatgt tggtaattca tatggatatt cagaaaaaac
     7921 tggtgtgtta aacactacag atattgaaag tgataaagta acattaaatg ttcctgcaac
     7981 agcagtatat caagagatat caaaaggaaa ttctgattta aatacacaag tagatagatt
     8041 atataatagt atgcttgcat gggaacaaat aatggattta gtttactcag aaagaggagt
     8101 atttaaaaca caagatttag atggtaatgg aactgtagat gataatgaat ttaacttaac
     8161 taaaaatgat atggctccaa aatctcgtat gaacataaaa taccaaagaa tgtttattgg
     8221 agcatttatg tatgcttctg gattacatgt aggagtagaa tatggttcag ttccagggct
     8281 tatgaatgga gtacctttcc aaatagatga tagtggaaaa gctacaggag gaaacttatt
     8341 tggatgggga ataggtcatg agataggaca tgttacagat attggaaaga tgacatatag
     8401 cgaaactagt aacaatgtac ttgctttact tgctcagacc tttgatgata agacacattc
     8461 aagacttgaa ggttcaacaa tggataagat atatgagcat gtaacttcaa atagcttagg
     8521 tataccaagc aatgtatttg aaaggcttgg aatgttatgg caattacatc tagcttatga
     8581 tgatgatttt acgggttcaa tgttaaagaa taactcagat gctgatttaa gtaatgatac
     8641 attctatgca aagatttcaa gaaaatacag agcactttca agtagtgacc caattaatag
     8701 tttaccaaaa gaccaaatgc tagtagcaat ggcatcaagt gttgttgaaa aagatttaag
     8761 agattacttt aaggcatggg gagtagaaat tacaccagaa ttaaacagta taatggatag
     8821 caagaactac gaaaaagaat caagaaagat acaatatgta aatgatgaag ctagaagaaa
     8881 gattttaaat gaaaatatag ctagtatggc aaaagatact aaagtaagtg ctagtttttc
     8941 tgatggaata caaagtggtt ctctagttaa atctaataaa atatctatag acctagatgt
     9001 agataaggat aaagataaga tactaggata cgaaataata agaagtgatg gaaattattt
     9061 agatggtgaa aatggaacac aggtaaaata cagaacagtt ggatttgtgg atgcaaaagg
     9121 aaatagcaag actaaattta ctgataatat atctcctctt aataacagag catttacata
     9181 taaggtagtt gcatatgatt atcacctaaa tccaacagaa gaatttgaag ttggtactgt
     9241 aaaattatca gatgaaggga aaataaataa atcagcatgg tcattcataa caaatactgt
     9301 ttctgacggt gatgttagaa ctgaaaatga ctcacatgga ccattacaaa atcctgaaat
     9361 tgataatata aaagataatg atgcaagtac tacatataaa ggaaagataa taagtaaagc
     9421 agagtggaac aaagaccctc aaaaagaccc tgatataaat gtagaagaaa atccatatat
     9481 aatagtagac atgaaagaaa cattacctat agtaggacta aaatatacaa aaccagagac
     9541 agaagctaga aagttctcat taaaaggatt atttaacttt agaaagaata ctagcacaac
     9601 atacaatcct ttaactaatt acaagataca agtaagtaat gataaaaaga actgggaaga
     9661 tgtaagtcaa ggaacttttg aatatggaca agatttagtt ggtggaggaa aagaccaaga
     9721 taatgaagct agagttacat ttaataaaga tggtaaactt tggacatacc aagctagata
     9781 tgtaaaacta atctcaaata ataagtctaa tatagaagtt gcagaattag atataatagg
     9841 acctccaggt gataatatag aaattggcat aataaataat gcagacgaaa ctcgtactaa
     9901 tggaattgga aaattagcag aagattatgt atatcaaagt gatgactcta gcacaagtga
     9961 agatgaaaca ggtaagattc cagcgggttc aatagttatt acaggtgaat acagtggaaa
    10021 tccagcattt aatttaccac tacttataga taaaaacaat aagactatat ctggagaagc
    10081 acttttattt gcagaagttc cagaaaaagg tgaactaggt gaaatcagta aaggtacatg
    10141 ggtttactat atgccaaaag aaaactttga ttctttaagt gataaggtta aggctgaact
    10201 atatagatac aatgaccttc aaggagatac tccagtaggt caaagatttg taagtgatac
    10261 aatgtatgta cctgtaggag ctaagaatta tgatgagtta aagacaataa gtcttactga
    10321 tagtaatagt aaagctagaa aagctacaag aaattctatt gatatcagca ataagaaggt
    10381 tataactgta agcaataaag taaagggtaa tattgttaga gataaatagt aggtggaact
    10441 ctaaaaacgt gaaaatacta acactaacat tgtatatcta atatgattag gtactagaat
    10501 agttttaaaa tagtagatat taaaagtaaa gtatataaat gtaaccataa gtttagggta
    10561 atttataaat taaatataaa agctaggtgg ataagtaaat ttatccactt agttaatgat
    10621 agataaggac gggtgttaat gagtaaaaga aaatctttta taagaactat agcagtatca
    10681 actatggcag tagctgtaat tggtagtgct acatgtgcat atgcagctcc agttttacaa
    10741 ggaacaaaaa cgtatgagaa agtaaatact atagatatat ctgtggactc agtagaaaat
    10801 atagtatatt catttcaagc cagtataaaa gtacaaggtg aggttgaagt agtagataat
    10861 gagcagaaag aaaagattac ttggtctgat aatataaaat ctcagataaa aagtggaaat
    10921 gcaaatgcta cttgtagagc agaatacaac aaatcaagta atacaactac attagatata
    10981 tatgttacat caaatgaaga tttactagac ggaaatagat tgaatatagg aagaatatct
    11041 gtaaaaaaat ctggcagtaa ctcaaatgct gactataaag ttttaggaaa aggaacaagc
    11101 gataaaccag ccttaaaaat tgtaacatat aacaacaaaa cagtgggtta tgagaatata
    11161 aactctgatg aaggattagt atttaccctt attaatgaga gtgaagtaaa accaattggt
    11221 ggaactggtt ctagtaaaaa tgaccctgaa aagtataagg tagagaaaag tgaagcactt
    11281 gaatatttat taaacaatat taggataaac tattctattg ttagtaaaga aacacaagaa
    11341 agtggttcaa atgtaatatt aaaacttgga ttagcacaaa aaacaactaa gggaagaaaa
    11401 gctacaataa acaagtatgt tgaagttaca cttccaaaaa gtttagaata catagttgaa
    11461 aatgaattat caaaaccgga tgaattacca ccagataatg gcaatggtgg aaataatggt
    11521 ggaggaagta attcaggtgg ctcatctagt ggtggaagct ctggaggagg aaattcaagt
    11581 gattctacat ctaatgtcac aatcaaaaaa ttaaaaggtg ctgatagatt tgagacagct
    11641 atcaaaatat ctcaaagtgg ttggacaaaa tcagatacag ttgtaatagt aaatggagaa
    11701 gataaaagta tggtggatgg gcttacagct acaccacttg ctagtgtaaa aaactcaccg
    11761 attcttttat catcaaatga aaagcttcct caaaaaactg tagaagaatt gaaaaggcta
    11821 aatccatcaa aagtaattgt gataggtgga aacaactcaa tgccaaattc agttgttgaa
    11881 gctatcaaag ctgtaaattc aaaaatatca gtacaaagaa taggtggaga tacaagatat
    11941 caaacttcta ttaatatagc aaaagaaata gataagacga ataatgtaag caagttgtat
    12001 ataggggcag gaaatggaga agctgattca ttatcaatag catcactggc tggtaaagaa
    12061 aaaactccaa tagtacttac acaaaaagat ggtgtagata atgaagctga acaatttatt
    12121 aaatcaaata aggtgtcaaa tatatacttt attggtggaa tagaaaaaat atctaacaag
    12181 gctattgaac aagttggaaa aatagcaaac aaagatatat caaaaaatag ggtggcaggt
    12241 caaactagac aagagacgaa tgccaaagta atagataaat tctactctca atctaaacta
    12301 gatggagttg tagttgcaaa tcaagataaa cttatagatg ctttagcagt tggaccatta
    12361 gcagctaaaa acaattcacc agttatactt gctacaaata cgttagataa atcacaagaa
    12421 tcgtcattaa aaggtaaaaa ttcatcaaag ttgtttgaag taggtggagg aatagcctca
    12481 tctgtaatag acaagattaa gagtttaatt gaaaaataaa tattaagtaa atagaaaata
    12541 agaatgtatt tagatataaa gttatatatt taaatgcatt cttattttat tttcattatc
    12601 ttaacaatct aataatattg aggttaaaat gtataataat ttgagctata ttaagtttaa
    12661 ttttaaataa agcagatatc ttttatatat ataaatataa attcaatttt gaatatgttc
    12721 aaaaagaagt taaaaataat tttttaaatt taataaaata aaacactaat atatagacga
    12781 gatatattca tatcatatat actctataat atatggaaaa taaagagaaa ataaatggaa
    12841 aataatcaca atataaatat aaaatataaa aatcatcagg gggatatgaa aatgaacaaa
    12901 aaaatattat cattaggtct agcagtatca ttaatcttag taaacttcaa aagtgtaaat
    12961 gcatcatcag tagtagaaaa aatatacggt aaagatagat atgaaacagc agcaaagata
    13021 gctgatagac agacttatga aacagtaatt ttagtaaata cagaaaaatc acttgcagat
    13081 ggattaagtg taagtggatt atcaggtgct acaaaagcgc caatactatt tactcaacaa
    13141 aataagatac cagcagatac aaatagatgt ttaaaaaata tcaaaaaagc atatataata
    13201 ggtacagaag ataccataag taaatcagta gaaaaagaac tagattctaa aaatatagaa
    13261 gttaaaagaa ttggtggaga agataggcta aaaacaagct atttaatagc taaagaaata
    13321 gcaactataa aaaaagtaga taaagtacta ttaactaatg catatagcgg agaagcagac
    13381 gcaatgagtg tatcatcagt agctactaga gatggagctc caatcatact tacagatgga
    13441 aagagtgtgc cttttgatgt aaaaaatatt caatcatatt gtataggttc agaggagata
    13501 atgagtaatc ctttagttaa aaatacaaac tcagtaagaa tagaaggaac tgaccgattt
    13561 gaaactaaca aaaacgtaat agactacttt tttaatagtg cagatggatt ctatgtatca
    13621 gatggatacc aattagtaga tgcaatagca gctgcaccac ttactaaaaa ctctccaatg
    13681 gtactagtaa atgatggaag cgataagact gtattagaag gagctaagaa tataacctct
    13741 gtaggtgaaa taaatgagaa ggtaatacaa cagtgtataa atgcttcaaa gtcaaataga
    13801 caaccaccaa caattacagt tggaagtaca gagatatata agggtgaaaa gtttgacact
    13861 ggcaagttaa atatagtagc taaagataac actgggaagg tattaccaat agaagttgat
    13921 gggtttatag atacaaatag agtaggtaca tacatattga cattaaaagc tacagacgaa
    13981 tggggaaaaa gcacaggaaa aagagtagaa ataaaagtgt tggatgacaa atctcatgat
    14041 tacaacagcc cagaatttaa aaaaatggta tctactgaaa tgtataactt aatcaattcc
    14101 tatagaaaag aaaaaggcaa agagcctttg gtagtatcta gtaagctaga aggtatggca
    14161 aatgcatggt ctaagtatat gatggataaa aaagtatttg cacattatat agatggaaaa
    14221 aatgctccac aagtattttc tgagtttgga atgagaagtg aggaaaatat agcatatatt
    14281 tatatagatt ctaagaacgt tcaaacgaca caagatgcaa aagacttggc aaaggctata
    14341 tttgaggtgt ggaagaagtc tccagagtac aatgcaaaca tgcttagtaa tgagttttat
    14401 agtactggat ttgggcttta tatattatct gatggtcaag tgcatgctac tcaagagttt
    14461 ttaaatggca atgaaggtag cttgtaaaaa attccataaa tcttgttttt ttgttaattt
    14521 actgatatta taaaatgaaa atcacatcta aagtactttt tgtaatctgt tttgttattg
    14581 atttacaggt tatggtattt taggtgtttt ttgttttagg agtttataaa ttttgaggga
    14641 atcactactg aggatgtaat ttatgattag ctgaattata taaaaaaatt agtaggagga
    14701 gttcgatgaa ggtaaataag agagtattat caataggatt gacaatatca ttaataatgg
    14761 caggagcacc aaatatcaat gcactttcaa gtatagaaaa gatacaaggg aaagatagat
    14821 atgagacagc tgccaaaatt gcacagaaac aaacatatga aaatgtagta ttagtaaata
    14881 cagataatac actagctgat ggattaagtg caagtggatt agcaggaaca gttaaagcac
    14941 ctatattatt atcacaaaga aacagtatac catcagacac agaaaaaatg ttaaaagatg
    15001 taaagaaggc ttatataata gggactgaag attcaatagg aaaatcagtt gaaaatgaat
    15061 taaagcaaaa aggaatagaa gttaaaagaa taggtggtaa tgatagaatt gaaacaagtt
    15121 atcttatagc aaaagaaata gcttcaataa aacctataga taaggttttt ataactaatg
    15181 gttatacagg tgaagcagat gctatgagtg cgtcatcagt agcatcaaga gatggagcac
    15241 ctataatttt aactaatggt aaaaatgttc catttgagaa aaaagaaggt gtgcaatgtt
    15301 atgctcttgg ttcagaggag ataataagta atgatttagt taaaaaaact aatgcagtta
    15361 gattagctgg agaagataga tttgagacta ataaaaaagt tataaaacat ttttatagtt
    15421 cagcaaaaga attttatctt tctaaaggtt atcaattagt agatgcagtt gctggttctt
    15481 ctatagctaa aaatgcacct attgtattgg ttgatggaaa tagtgataag tctgtactta
    15541 gaagtgctga taagattaca gcactaggag gaatagatga aaaaacacta gaacaatgtt
    15601 taagtgcatc atcactggat gcaagtgcac caacaataac tgttggaaat ttaaatattt
    15661 atcaaggtga taagtttgat ataagtaaac tgaatatcgt agcaaaggat agcaatggta
    15721 atgatttgac accagagctt ataggaaata ttaatactga taaggttggt aaatacaaag
    15781 ttactataaa agctactgat attggtggaa agactacctc aataagtgta gaagtaaatg
    15841 tacttgaata taagactaat gatatgaata gtagtgagtt taaaagaatg gtttctagtg
    15901 aaatgtatag tttggtaaat tcatatagaa aagaaaaagg gaaagaacca ttacaagttt
    15961 ctaaaaactt agaagggctt tctaattcct ggtctaagta catggcagat aaaggtcagt
    16021 tttcacatgt aatagatggt aagaaatctg ttgaagtatt tactggatat gggttgagaa
    16081 gtgaagaaaa tatagctttt gtaccacttg ttacaaagag cacttatacg acaaaggatg
    16141 ctagagaagt agcaaatgta atatttacag tttggaaaaa atcggataaa tataatgaaa
    16201 atatgctaaa cagtgatttt gtatatactg ggtttggact gtatatattg cctaatggag
    16261 aagtgtatgc tactcaagaa tttttaaata agtaaaatta aattttttaa gttattaaat
    16321 attgaaattt aagggtataa aaactcatat gcttgggaaa aataataatt tcaaaaaaat
    16381 gccttgcaaa aagaattaaa atggattatt atagagatgt gagaaatatt aggaatatat
    16441 ggatgattat tctatgtaca taataaagag atgtaatttt aatataatgt tgggaggaat
    16501 ttaagaaatg aataagaaaa atttagcaat ggctatggct gctgttacag tagtaggttc
    16561 ggctgcacca gtatttgcag ctcaaactga ttaccaaaac acatacactg tagtgcaaag
    16621 caagtatgaa aaagcattaa aagatataca aaaagggatt acagataaaa aaataactga
    16681 agtaggaata tcttatgaag gtaagccagt tactactatc actgttgcaa atatggatac
    16741 aacaggaact acttcaacaa aagaagagtt agcaagtgct ttattaaaga ctactgttaa
    16801 tgataagtta gataatttag gtgatggaga ttatgtagat tttgatataa cttatgttgg
    16861 tgctgctgac gcacttactg caggagatct taatgccttt aaagcagcct tagcagctga
    16921 tactacattg aaaatacctg ttgcttctac aacaaaattt ggagctgttg tattaggaac
    16981 aggagataca aaacttgatc cagcaagtag tgcagttaat gtaagtactg caatagaagc
    17041 taacattgtt ggaaatactc ttacagtaag caaaaaagct tctgacgcaa ctaaaattgg
    17101 aaaagaagat gaagataatt ctacagctac agacgttaca tttaaagatg atgcaaagat
    17161 atctgtatca gttggagatc caaaaataga tttagcaaaa tcatttgctt ttgatgacac
    17221 aactggtaaa ctagatggaa tagtagaaaa agaaaatact gcaacatcac atgcatatgt
    17281 aagagtcata aatgctaaag agcaaacaat agacttagat gctagttcat ataaatcagc
    17341 tgaagattta gctaaagctt atgcatttga tgtaaatgag cttaagactc tttacactga
    17401 aatagaagca tatcaaaaag atagtaataa taaaactgat aaagttcaaa tagttgatgg
    17461 taaatatcaa acaatacttt atgctgaagg aaaaagatta actactaaat cagcaactca
    17521 agcttctaaa ttagcagatg aaaattcacc acttaagtta gttataaaag cagataagtt
    17581 aaaagattta aaggattatg ttgaagattt aagaaatgct aataatggtt actcaaatac
    17641 tgttactgta gcaggtgatg atagaataga aactgcaata gaattaagta gtaaatacta
    17701 taataattca gatgaagaca atgctataac agaagatgct gttaataatg ttgtactagt
    17761 tggatctcaa gcaatagttg atggacttgt tgcatcacct ttagcttcag agaagaaagc
    17821 tcctttatta ttaacttcaa aagataaatt agattcaaat gtaaaatctg aaattaaaag
    17881 agttatgaat ttaaaaacta caacaggaat aaacaattct aagaaagttt acctagctgg
    17941 tggagttaat tctatatcta aagaagttga aaatgaattg aaagatatgg gacttaaagt
    18001 aacaagatta tcaggagatg atagatatgc tacttcatta gaaatagctg atgaaatagg
    18061 tttagatgat gataaagcct ttgtagtagg tggaacagga cttgcagatg ctatgagtat
    18121 agctccagtt gcttctcaat taaatcaaaa aggtgatgct acaccaatag ttgtagttga
    18181 tggaaaagct aaagaattaa gctcagcagc tgaagatttc ttagatgatt cacaagttga
    18241 tataataggt ggtaaaaata gtgtttctaa agacatggaa gatgctatag atgatgctac
    18301 aggaaaatct ccaaatagag ttagtggaga tgatagacaa gaaactaatg cagaagtatt
    18361 aaaagaatct gattatttcc cagatggtgc agtaaattac tttgttgcta aagatggatc
    18421 tacaaaagaa gatcaattag ttgatgcatt agcagcagca ccagtagcag ctaattttgg
    18481 aagaacttat aatataaaag ataatgatag ttctggaaca gtttctccag caccaatcat
    18541 attagcaact gattcattat cttcagatca aaatgttgca ataagtaaag cattaccttc
    18601 tggaaaatca ggaaataatt tagtacaagt tggtcaaggt atagcaaatt cagtaataaa
    18661 taaaataaaa gatttattag atatgtaata taaattttaa atatagagta aaaaagattc
    18721 cttaaatagg aatctttttt atatttgaaa tttgtatcaa aaataaaaga gagtctagat
    18781 atctagtctc tctttttgtg agaaatactt aaatgattaa aagatgtaat tcatttaagt
    18841 atgattatta tactataaat aaaaaaagtt gtctactatt ccacattttt aaggtataat
    18901 aatagttgta tgataaaaaa ggaggtaaga aatacatgtc agttttagat acaatacttg
    18961 ataaagcaga tgaacaagaa attaagaagt tgaatttaat agtagataaa atagaggctt
    19021 tggaagaaga aatacaaaac ttatctgatg atgaattgaa agaaatgact aatgtattta
    19081 ggaatagatt aaataatgga gaaactttag atgacatatt aatggaagct tttgcagttg
    19141 taagagaagt atcgaaaaga aaattaggaa tgcgtcaata tagggtacag ttaattggtg
    19201 ggatagtaat acatcaaggt aaaatcgctg agatgaaaac aggtgaaggt aaaacgttag
    19261 ttgaagtagc accagtatat ctaaatgctc ttacaggcaa gggtgtacat gtaatcacag
    19321 taaatgatta tctggcagaa cgtgataaag aacttatgag accagtttat gaatctcttg
    19381 gtatgacagt aggagtaatt atatctaacc aagacccaaa tataagaaaa caacaatata
    19441 aatgtgatat aacttatggt acaaatagtg aatttggatt tgattattta agagataata
    19501 tggtgccaga tttatcttat aaagtacaaa gagaactaaa ctttgccata gtagatgagg
    19561 tagactcaat attaatagat gaagctagaa ctccacttat tattgcagga gatggagatg
    19621 aagatttaaa gctttatgag ttagcaaata gttttataaa aactgttaaa gaagaagact
    19681 ttgagatgga tagaaaagac aaaactatag cattaacagc aagtggtata agtaaagctg
    19741 agtcattttt tggtataaca aaccttactg atataaagaa catagaatta tatcatcata
    19801 taaatcaagc tttaagaggt cataagctta tggaaaaaga tgttgactat gttatttcaa
    19861 atggagaagt aatgatagtt gatgaattta caggaagagt aatggatgga agaagatata
    19921 cagatggact tcaccaagct atagaagcta aagaaggtgt tgaaataaaa aatgaatcta
    19981 aaaccatggc tacagttact tatcaaaatt tctttagatt atatgaaaag ctttctggta
    20041 tgactggtac tgcaaagaca gaagaagggg aatttgagtc aatctataaa ttaaatgttg
    20101 tccaaatacc aactaataaa ccagtgatta gagctgattt gcatgataag gtatttaaaa
    20161 cagaagaaga aaagtatagt gctgttgtag aagaaataat aaggatacat aagactagac
    20221 agccaatact tgtaggaaca gtttctgttg aaaaatctga gaagctgtct aaaatgctta
    20281 aaaaacaagg tattaagcat caagtcttaa atgccaaaca acatgataaa gaggccgaga
    20341 taatttctaa agctggtaaa ttagatgcta taacgattgc tacaaatatg gcaggtagag
    20401 gaaccgatat ttctctaggt gcaggagata aagaagaaga gcaagaagtt aaaaatttag
    20461 gtggacttta tgttatagga acagaaagac acgaatcaag aagaattgat aatcagctta
    20521 gaggacgttc tggtcgtcaa ggagatccag gtacatcaag attctttgta agtcttgaag
    20581 atgatgtaat aaagctttat ggtggaaaaa ctatagagaa acttatgaag agaacaagtt
    20641 caaaggaaaa tacggctatt gaaagtaaag cacttacaag agctatagaa agagcgcaaa
    20701 aaggtgtaga aggtaaaaat tttgaaataa gaaaaaatgt tcttaaatat gatgatacta
    20761 ttaatgagca aagaaaagtt atatataatg aaagaaataa agtgttaaat gatgaagata
    20821 tacaagaaga tattcaaaaa atggttaaag acatcataca agaagcagga gaaacttatt
    20881 taattggaag aaaaagagat tattatggat attttaaaca tttatatagt acatttatgc
    20941 cagcagatac attactaata cctggtgtag ataaaaaaag tgtccaagag ataattgata
    21001 gcacatatga aatttcaaaa agggtttatg acttgaaaaa gatgatgatt ggtattgata
    21061 aggttgcaga gttagaaaaa acagtacttt taaaagtggt tgatcaatac tggatagacc
    21121 atatagatgc tatggaacag ttaagacagt atataggtct taaatcttat gctcaaaaag
    21181 atccatttaa ggaatatgct ttagaagggt atgacatgtt tgaagcttta aataaaaata
    21241 taagggaagc aacagtgcaa tacttatata aatttaacta aaaaagttca tgtaattttt
    21301 attaaatgaa aaataaagta ttttgattct ttatattgta attacaatta aataattaag
    21361 gaataaaaat gaaaaagaag ctgaaaatca gcttcttttt ctgtgtgaga aatacggaaa
    21421 aaagagatgc ttgaaatttc tttcttccat ttaatattat actataaaga agatgaaaag
    21481 tctattagtt ataaactgga ttattaaaaa aaattacaat tttgttactt ttttgtatac
    21541 ataaatatta aaaaaataaa atataagaaa aagtttgtat cttttggtta attattacaa
    21601 taaatctcat ttattgaaat aatatcaaat atatattata attggtaaat aaggaaaaaa
    21661 taataaaaat ttgaattttt tagggggaaa ataccatgaa taaaaaaaat ctttctgtaa
    21721 ttatggctgc tgcaatgata agtacatcag tagctccagt ttttgctgca gaaactacac
    21781 aggtaaaaaa agaaacaata actaagaaag aagctacaga attagtttcg aaagttagag
    21841 atttaatgtc tcaaaagtat acaggaggtt ctcaagttgg acaaccaata tatgaaataa
    21901 aagttggtga gactttatca aaattaaaaa taataactaa tatagatgaa ttagagaaat
    21961 tagtaaatgc tttgggagaa aataaagaac ttattgtaac tataacagat aaagggcata
    22021 taacaaatag tgcaaatgaa gtagttgcag aagcaattga aaaatatgaa aattcagcag
    22081 acctttccgc tgaggctaat tctataacag aaaaagctaa aactgaaact aatggaattt
    22141 ataaagttgc agatgtaaaa gcttcatatg atagtgctaa agataagtta gttataactt
    22201 taagagataa aacagaaaca gtaacttcta aaactatata tgtaggtatt ggtgatgaaa
    22261 aagttgattt aacagcaaat ccagttgatt caactggaat aaatttagac ccttctgcag
    22321 aaggatttag agtaaataaa atcgataaat taggtgtagc aggagctaaa aatattgatg
    22381 atgtccaatt agctgaaata actataaaaa atagtgacct aaatacagtt tcaccacaag
    22441 atttatatga tggatataga ttgactgtta aaggtaatat ggtagcaaat ggtacatcaa
    22501 agtcaattag tgatatttca gcaaaagatt cagaaacagg aaaatataaa tttattatta
    22561 agtatactga tgcatctgga aaagcaacag agcttactgt agagagtact aatgaaaaag
    22621 atttaaaaga tgccaaagct gcattagaag gtaattcaaa ggttaaattg atagctggag
    22681 atgatagata tgcaactgca gtggctatag caaaacaaac aaaatatact gacaatgtag
    22741 ttatagttaa ttcaaataaa ttagttgacg gattagcagc tacaccactt gctcaatcta
    22801 aaaaagcacc tatattgtta gcatctgata atgaaatacc aaaagtaact ttagattata
    22861 taaaagatat aattaagaaa agcccatcag ctaaaatata tatagtaggt ggagaatcag
    22921 cagtatcaaa tacagctaaa aagcaattag aatcagtaac taagaatgtt gaaagactag
    22981 ctggagatga tagacatacg acttctgtag cagtagcaaa agctatgggt tcttttaaag
    23041 atacatttgt agtaggtgca aaaggggagg ctgatgctat gagtatagct gctaaagctg
    23101 ctgaacttaa ggctcctata atagtaaatg gctggaatga tctttcagca gacgctatca
    23161 aattgatgga tggaaaagag attggtatag ttggtggctc taacaatgta tctagccaaa
    23221 ttgaaaatca acttgccgat attgataaag atagaaaagt tcaaagagtt gaaggagaaa
    23281 caagacacga tactaatgct aaagttatag aaacatatta tggcaaatta gataaactat
    23341 atatagcaaa agatggatat ggaaataatg gtatgctagt agatgcatta gcagcaggac
    23401 ctctagcagc aggtaaaggt ccaatacttc tagctaaaac tgatataaca gactcacaaa
    23461 agaatgcact tagtaaaaaa ttaaatcttg gtgcagaagt aactcaaata ggtaatggag
    23521 ttgaattgac agtaatacaa aagatagcta aaatactagg ttggtaataa cttatactgt
    23581 ggaaaagatt ccttaattaa tacaggaatc tttttctata attatgctat ttattgtaaa
    23641 ttataacatt ttataaagta ggaggatgta gtagtggtag atgcaacttt attttttaca
    23701 ccacatcaag atgatgaaac cttaagtatg ggaagtgcta taattgaaca tgttgaaaaa
    23761 agtgatacac atgtcatatt atgcacagat ggtagtaaat ctataataag aaaagttttg
    23821 gatgatggag ggtcatgttc ttatcatatt aaagatgttc ataaatattc tttatcagaa
    23881 agtgagtttt caaaagatag agatgaagaa tttaaggata gttgtgaagc aatgggtgta
    23941 aaagaaagta atatacatat agaagataat agagcccatg atggtgagct aagtaaggaa
    24001 aaggcaaggg aaataatact taagtactta gaagaatatc cagatgcaaa agtaaaaact
    24061 gttactccat ttaaagctag tggaatacat gaagaccata gagcattagg agaagctgca
    24121 ctagagttat ataaagaagg caaaataaaa gatttaaggt tttatgtaga accatatgac
    24181 tataaggatt ttaaaaaagt taatccaaat gtagaggtat ggaaggtatt acctagtcaa
    24241 gaagagaaat tattaagtgc aatgaatgca tataaaaaat ggaatcctga aagtggacat
    24301 tatgctatag gatatcattc agtaaaatct cattttgatg agctagctat aaataaaata
    24361 cagtacgtac atgctccata aaatattata aaagaattag gaggtaaatt ttaatgaaaa
    24421 tatcaaaaaa gatagtggca ttattgacta taacattttt aactattaca ttatatggaa
    24481 atacatctaa tgcatccaca aaatacacgt taacaggttc tggaagacgg gaaacagcaa
    24541 taaaaataag tcaagcagga tggaaaaagt ctgaaaaagc tgtgctagta aatgataatt
    24601 caatagcaga tgctttatca gctactccat ttgctaaagc aaaagatgcg ccaatattat
    24661 taactcaaag taataaattg gatagtagaa caaaagcaga attaaaaaga cttggtgtga
    24721 aaaatgtata tttaataggt ggttcaattg cattaagttc agagattgaa aagcagttaa
    24781 atgcagaaaa tataaatttt gaaagaatat ccggaaatag tagatatgat acttctttaa
    24841 aattagctga aaagttaaat aaggaaaaat ctatatctaa aatagtagta gtaaatggag
    24901 aaaagggtct tgctgatgca gtgagtgttg gagctatagc tgctcaagaa aacatgccaa
    24961 taatactttc tgattcggat aatggaactg aagtagctga taattttata gatagtaaag
    25021 atatagaaaa atcttatgta ataggtggta catattctat ttctaattct gtagaaagaa
    25081 gcctaccaaa tgcaacaaga atagcaggta gtagtagaag tgagacaaat gcaaagatta
    25141 tagaagagtt ttataaagat actgatataa aaaatattta tgttacaaaa gatggtacaa
    25201 ggaataagaa tgatttaata gattctttag cagtgggtgt attagcagct aaaaatagtt
    25261 ctccaatagt attagcagga aataaacttg atactgttca aaaagatgtt ttaaatacta
    25321 agattataga taaagttact caaattggtg gtttaggtaa tgaaaatgtt gtagaagata
    25381 tactagatat acaagaagaa actaagtata ctgttgaaac tattgatgag cttaatgtag
    25441 ttataaaaaa agcagatgca aatgatatta taaaatttgt accagaaaag gatagaagta
    25501 taaatagctc atttaaaata gaaacagata aagctataga ggttgaatta aaaggtgttt
    25561 ataagcaatc aattactata aatatgccaa atggtaagat tagtaactta ggagaaatca
    25621 atggtactac agaaataaaa aatataaaga ataactcatt tataaataag ggaagtatat
    25681 ttgacttgga tatttcttct aacaatgaat gtaaaataga aaatgaaagt tctggtgaaa
    25741 tatggcttat aactatattt caaaatgcaa aaggagttta tattgtaaat gatggcgata
    25801 taacaaaaat atcaaataat tcttctgatg ttataataaa gaattctgga gagatagata
    25861 cagtaactgg tagtaaagag cctgccataa gtggtaaaaa gccaagaata aatgacacta
    25921 aagttgatga cacacttgca agaggtcttt atgttaaagc tgattcatgc tacaatcctg
    25981 aaaaagatta tgtaatggtg accatattaa gtgaacctaa aagttcagac tatagtattt
    26041 attatagagt agtagaaaat aaaccatctg ctatgtatgt aggaaacaag attaatcctg
    26101 cagattggga tattgttaca aaatataata aagaatttat tgaaaaagcc aaagatggat
    26161 catacataga agttgttgag gtaaactctt taacaaataa agtctcaaga tggggaaaat
    26221 ctaatgtaac taatgatggt gttagataat gtaatcaata agcagataat tattgtttag
    26281 ttatctgctt attaattttt aaaaattatc attttactaa acaaataatt taaaactata
    26341 acgactatat tagtaaatat ttttacaata acatcattta caaaaagcaa atcaatcatt
    26401 acatacataa ttaacatttc aacgacacca gaaagtagcc taaaactaac aaaagacaaa
    26461 aattccttaa ataagaactt tatttcaatt cttttactct caaacacaaa aaacttatta
    26521 gtaacataag caaataaaac agcaagtatc catgccaaag catttgcaac cataaaatta
    26581 aataaaataa ctctagtaaa aaataaatac gaaactatat ttacaagagt agtaaatgct
    26641 ccaaaaaata aatataatat tgtctcttta tgttttttaa aaatcaaata taaccctcct
    26701 aaactaatca aatataacta gctttaaata aaattaacac attttagtat aacacatcta
    26761 ttaataaatc aatatatctt aaaatattat aaaaataaaa ctataatatt ctataattaa
    26821 tatgtactca taatccatat tttatcaaaa taaaatatga ccttaattta gaaatctgat
    26881 ataataaaaa ttaaatacat ataagggggt aaacatgaga aagtacaaat caaaaaaatt
    26941 gtcaaagcta ctagcattgt tgacagtttg ctttttaata gtgtcaacaa tacctgtctc
    27001 agcagaaaac cataaaactc tagatggagt agaaactgca gagtattctg aaagctatct
    27061 tcaataccta gaagatgtca aaaatggaga cacagcaaaa tataatggag taataccttt
    27121 tccacatgaa atggaaggta caacacttcg aaataagggt agaagtagtc ttccatcagc
    27181 atataaatca agtgtagcat acaacccaat gaatcttggt cttactacac cagcaaaaaa
    27241 tcaaggaagt cttaatacat gctggtcttt ttcaggtatg tcaactttag aagcatatct
    27301 taaattaaag ggatatggaa cgtatgactt atcagaagaa catttaagat ggtgggcaac
    27361 tggtggaaaa tatggatgga acttagatga tatgtcaggt agttcaaatg taacagccat
    27421 aggatatctg acagcatggg caggtcctaa gttagaaaag gacataccat ataatcttaa
    27481 atctgaggca caaggcgcaa ctaaaccttc aaatatggat actgcaccta ctcaatttaa
    27541 tgtaacagat gttgttcgtc ttaataaaga taaggaaact gtaaaaaatg ctataatgca
    27601 atatggttca gtgacatcag gttatgcaca ttattcaaca tattttaata aagatgaaac
    27661 agcatataac tgtacatata agagagctcc attaaatcac gctgtagcga tagtaggatg
    27721 ggatgataat tattcaaaag ataactttgc atctgatgca aaaccagaat caaatggagc
    27781 atggttagta aaaagtagtt ggggagagtt taattctatg aaaggattct tctggatttc
    27841 ttatgaagat aaaactctac taacagatac agataactac gcaatgaaat cagtatcaaa
    27901 accagatagc gataaaaaaa tgtaccaact tgaatatgct ggtcttagca agataatgtc
    27961 aaataaagta acagcagcaa atgtatttga ttttagcaga gattctgaaa aacttgactc
    28021 tgttatgttt gaaacagact ctgtaggagc aaaatatgaa gtatattatg caccagtagc
    28081 aaatggagtt cctcaaaaca attcaatgac aaaacttgca agtggaacag tatcatattc
    28141 tggatacata aatgtaccta ctaattctta cagcttacca aaaggtaaag gagcaatagt
    28201 agtagttata gacaacacag caaatcctaa tagagaaaaa tcaactttag catatgaaac
    28261 tgatatagat ggatattatc tatatgaggc taaagcaaac ttaggtgaaa gctacatact
    28321 tcaaaacaat aaatttgaag atataaatac atatagtgaa ttttctcctt gtaattttgt
    28381 tataaaagct ataacaaaaa catcttctgg acaagctact tcaggagaat ctttaactgg
    28441 agcagataga tatgaaacag cagttaaagt tagtcaaaaa ggatggactt cttcacaaaa
    28501 tgcagtatta gtaaatggag acgcaatagt agatgcttta acagctacac catttacagc
    28561 agcaatcgac tctccaattc ttttgacagg aaaagataac ttagattcaa aaactaaggc
    28621 agagttacaa agattaggaa ctaaaaaagt ttatctaata ggtggagaaa attctttaag
    28681 caagaatgta caaactcaac ttagcaatat gggtatatca gtagaaagaa tttcaggtag
    28741 tgatagatac aagaccagta tatctctagc tcaaaagtta aatagtataa aatctgtttc
    28801 acaagttgca gtggcaaatg gtgtaaatgg acttgcagat gcaataagtg ttggtgcagc
    28861 agctgctgat aacaatatgc caataatact tactaatgaa aaaagtgagt tgcaaggtgc
    28921 tgatgagttt ttaaattcat ctaaaataac taagtcttat ataattggtg gtacagctac
    28981 tttatcatca aatttagaaa gtaagctttc aaatccaaca agacttgcag gaagtaatag
    29041 aaatgaaact aatgctaaga taatagataa attctaccct agctcagatt tgaaatatgc
    29101 ttttgttgtt aaagatggtt caaagagtca aggagacttg atagatggac tagcagtagg
    29161 agcattgggt gctaaaactg attcaccagt agttttagtt ggaaataagt tagatgaaag
    29221 tcaaaaaaat gtacttaagt ctaagaaaat agaaactcct attagagttg gtggaaatgg
    29281 aaatgaaaat gcttttaatg aactaaatac tcttttagga aaatagtgaa tggaaagata
    29341 gtaaggatat acattttatt attaaaaaat attcaataga agtttataaa tataaaataa
    29401 caaaaagggg tgtctcaaaa tgaacttttt agttcatgag gcactctttt ttgttgtttt
    29461 atatttttat catttaaata atgaaaaata ggattgtcta tatttttaga tagttttctt
    29521 attattgatg aaaaatgtaa taattttaaa ctgtaaaata gtgtaaagtt attttataat
    29581 ataaatatag taagatttta aacaaaataa gggggaataa taatgaaagc accaaaaact
    29641 attttaacaa tactgacaat agcgcttact ttaagtagca tttctataat accatcatat
    29701 gcacttacag aggaaaaatt aataggtaat ggtagatatg aaactgctgt aaagataagt
    29761 caaaaggcat acagttctag ttctaatgta gtgctagtta atgacaattc attagctgat
    29821 gcactttcag ctacaccatt tgcaaaggct aaaggagcac caatactttt gacagaaagt
    29881 gataagctag atgataggac tgaaaaagag ataaaacgtc taggagctaa agatatttat
    29941 ttgataggag gtgctgctgt acttaataaa gatatagaga acaagttaaa aggtaatgga
    30001 cttaatgtag aaagaattaa tggtaaggat agatatgaaa cttcactgat tttggctaat
    30061 aagcttaagg atataaaaga tattaaggaa gttgctgtag taaatggtga aaaaggattg
    30121 agtgatgctg ttagtgttgg agcaccagca gctcaaaata agatgcctat aattttgtct
    30181 agtcctaaag atggagtaga agcatttgat aaatttataa gagatgagaa agttataaaa
    30241 gcatatgtaa ttggaggtac aaattctgtt tcaagagctg tagaaaagag tcttcctaat
    30301 gcagaaagat tgagtgggaa agatagaaat gagactaatg ctaaggtcat agaaaagttt
    30361 tatactgata caaacttaag taatttatat gtaactaagg atggtagtaa aaatgagaat
    30421 caactaatag actcgttagc tgttggagta ttggctgcaa aaaatgagtc tcctgttgtt
    30481 ttggttggta ataaattaaa cacaaaacaa agagatatac ttagtactaa aaaattaaat
    30541 actataacgc aggtaggtgg aaatggaaat gaagaagctt ttgatgaaat taaaagccta
    30601 caagaaaaaa ctgtgtttga agcaaaaact gttgaagaat taactgatat gataaatata
    30661 gcaagtccta atgatattat aaattttaaa ccaaatgaga atactgtaaa tgaagctttt
    30721 agaatggtaa caaataaacc aataactgtg aatataaagg gtgattgttc aaagactctt
    30781 acagtagata tgcctaatgg ggaagtgaat aactatgcta ctttggtaaa tgttatagtt
    30841 agaaatattg gagaaggtgg atttaataac catgatacaa tcactatatt gtcagttcgt
    30901 gacaaaaatg gtagagttat agaaaatact agaaattcag atatagatac gttgatgata
    30961 ttagcttctg ctaatgatac aaaattgatt aatgatggat atataggaaa acttatagat
    31021 aattcatcta atagtgatat aactaataat ggtactatag ataaaaaagt taatcaggtt
    31081 gaggatttag aggctaaggt tgattcaatt gaaaaagcta tagattctat aagtcagaaa
    31141 gtcaataaaa tacaagatat actagataag tttggatttc taaagaaatt tcttagttaa
    31201 gaatatgtat atttaagtga agtgtgtgtt gtaattgttc taaagttata tagttaaaca
    31261 ggtttatata caaacagact tatagataaa tatgtagttc aatatatatt tgaataagct
    31321 ttggggaggt aacatgggga ttggaattga atgtaacaaa aaaataaata aaagtattga
    31381 caaaactaga ataaagaaaa ataatct
//
LOCUS       DAEMGG010000037        30999 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEMGG010000037 DAEMGG010000000
VERSION     DAEMGG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.037"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            68..141
                     /locus_tag="KPK24_001600"
     tRNA            68..141
                     /locus_tag="KPK24_001600"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Gly,seq:tcc)
     gene            150..266
                     /gene="rrf"
                     /locus_tag="KPK24_001601"
     rRNA            150..266
                     /gene="rrf"
                     /locus_tag="KPK24_001601"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            540..2558
                     /locus_tag="KPK24_001602"
     CDS             540..2558
                     /locus_tag="KPK24_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2299280.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            3066..5078
                     /locus_tag="KPK24_001603"
     CDS             3066..5078
                     /locus_tag="KPK24_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2299281.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKIYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5320..7233
                     /locus_tag="KPK24_001604"
     CDS             5320..7233
                     /locus_tag="KPK24_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299282.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKVTTYISFVKKELVKYWPLIIEKCDGISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            7226..9445
                     /locus_tag="KPK24_001605"
     CDS             7226..9445
                     /locus_tag="KPK24_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG2299283.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKSGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLSLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9554..10384
                     /locus_tag="KPK24_001606"
     CDS             9554..10384
                     /locus_tag="KPK24_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG2299284.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10374..13841
                     /gene="addB"
                     /locus_tag="KPK24_001607"
     CDS             10374..13841
                     /gene="addB"
                     /locus_tag="KPK24_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG2299285.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNQTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIINFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13841..17668
                     /gene="addA"
                     /locus_tag="KPK24_001608"
     CDS             13841..17668
                     /gene="addA"
                     /locus_tag="KPK24_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG2299286.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKSDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17714..18925
                     /locus_tag="KPK24_001609"
     CDS             17714..18925
                     /locus_tag="KPK24_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG2299287.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18912..22439
                     /locus_tag="KPK24_001610"
     CDS             18912..22439
                     /locus_tag="KPK24_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2299288.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEIPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22570..23823
                     /locus_tag="KPK24_001611"
     CDS             22570..23823
                     /locus_tag="KPK24_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG2299289.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            23972..25024
                     /gene="ytvI"
                     /locus_tag="KPK24_001612"
     CDS             23972..25024
                     /gene="ytvI"
                     /locus_tag="KPK24_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG2299290.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            25114..26340
                     /gene="pepT"
                     /locus_tag="KPK24_001613"
     CDS             25114..26340
                     /gene="pepT"
                     /locus_tag="KPK24_001613"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG2299291.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPKIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            26547..27599
                     /locus_tag="KPK24_001614"
     CDS             26547..27599
                     /locus_tag="KPK24_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG2299292.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADNISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(28017..28586)
                     /gene="yfcE"
                     /locus_tag="KPK24_001615"
     CDS             complement(28017..28586)
                     /gene="yfcE"
                     /locus_tag="KPK24_001615"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG2299293.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(28611..29411)
                     /locus_tag="KPK24_001616"
     CDS             complement(28611..29411)
                     /locus_tag="KPK24_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG2299294.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            29546..30406
                     /locus_tag="KPK24_001617"
     CDS             29546..30406
                     /locus_tag="KPK24_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299295.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            30406..>30999
                     /locus_tag="KPK24_001618"
     CDS             30406..>30999
                     /locus_tag="KPK24_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG2299296.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYIN"
BASE COUNT        12948 a         3125 c         5378 g         9548 t
ORIGIN      
        1 gatatatatg tagtttttag agtgctaact ctaaaaaatt agttttacta atttataaaa
       61 atttaatgcg ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt
      121 cgatcccctt cacccgctcc aaaagattat gtggttacaa tagcagagag gatacacctg
      181 ttcccattcc gaacacagaa gttaagctct ctagcgctga tggtacttgg tgggaaactg
      241 cctgggagag taggacgtag ccacgtaatc tttttttgtt gtattaaatt taataaacct
      301 acaattgtta tatgttttta tttgtctttt atagattttt ttggcaataa atgatatagt
      361 gaagataatt aaatatattc aatatattta atttttttat tttttgtttt tataattttt
      421 ttagaagttt attttttatg tacttattta taattttggg taaaaacact ttgatataat
      481 ttcaaaaatt aaaaggttaa aaaacaaagt agaaatttat gttaaataag gagattaata
      541 tgagaagaaa tacaaaatta ttaacaacag ggattctttc aatggcaatc gtcgcaccta
      601 caatggcatt tgctactgaa tctaatgcta tggaaaataa cgctgattta aatataaact
      661 tagagaaaaa aagtatcgtt ttaggtagta cctcaaaagt cagtgttaag tttaaagaaa
      721 aaccagatgc agatagcatt acattaaagt ataaatgcta tgatatgcca ttggatacaa
      781 ctttaaacta caatcaatca actgagtcat atgaaggaac tatcaattat aataaagacc
      841 cagaatatct aaatgtttgg gaactacaag ggataacaat aaacagcaaa aataatccta
      901 aaactttaaa caaacaagaa ctagaaaaga tgggattaaa tttaaaagac tataatgtaa
      961 cacaggaatg tataattgaa gatataactt ctagaaaaga tgtaaataaa tatttaagaa
     1021 aaacttctgc acctattaca gaacttacag gaagtgatag atacgaaaca gcagttaaaa
     1081 taagtaaaga aggctggaaa aatggttcag ataaggtagt tataataaat ggggatgtaa
     1141 gtatagatgg cattatatca actccactag caactacata taatgcgcca atacttttgg
     1201 ttgaaaaaaa caatgtacct aatagtgtaa aatcagaatt aaagcgttta aatcctaggg
     1261 atgtaattat aattggagat gagaatgcta tttctaaaac tactgctaat caaattaaat
     1321 cgactgtaaa tgctagtcaa acacgtctaa agggctctaa tagatatgag acatctttat
     1381 tgatagcaaa ggaaatagat aaaaatcatg atgtggaaaa agtgtacata acaaatgcta
     1441 atggcggaga agtggatgca cttactatag cagcaaaagc aggtcaagac aagcaaccaa
     1501 ttatattaac tgataaaaat agtattacag acaacacata taaatggtta aagagtgagg
     1561 atttacaaaa tgcttatttt ataggtggcc ctcaaatgat atcaacaaat gttataaata
     1621 aggtaaatga tataactaaa gataatgtta ctaataatag agtatatgga gcagatagac
     1681 acgaaacaaa tgcaaacgta ataaaaaaat tctatacaga tgatgagcta gaggctgttt
     1741 tagtagctaa atcagatgta cttgttgatg ctttagcagc aggtccattg gctgccaact
     1801 taaaatctcc aatacttata acaccaaaga cgtatgtatc tgcataccat aaagaaaact
     1861 tagaagctaa atcggctaat aaggtatata aaataggagg aggattgact tctaaggtaa
     1921 tgaactctat agcatcatca ttatctaaac acaatacgac tccaacagaa ccaggaaata
     1981 gtgggggtaa gacagttatg attgacccag gacatggtgg ttcggacact ggaacaacag
     2041 gtaaaccatt agggggtatt aaagaaaaag actatacatt aaatacttca cttgcgacaa
     2101 ctgaatattt acgttcaaag ggattcaatg taataatgac aagagataca gataagactt
     2161 tatctcttgg aaatagaact gctttatcta actctttgag accagattta tttacgagta
     2221 tacactataa tgcatctgat acaactggga atggtgtaga agtattttat aagcttaaag
     2281 ataaagatgg aggaactact aagactgtag ctacaaatat attaaataga atcttagaga
     2341 aatttaatct aaaaaataga ggtgcaaaaa caagaacact atcaactgac cctacaaaag
     2401 attatttata tgttttaaga aataatgata tgccagctgt acttgtagaa tgtgcatttt
     2461 tggataatga gaaagatatg agtttactta atacatcaaa taaagtaaaa gaaatgggta
     2521 cacaaatagg taaaggcata gaagattcat taaaatagtc tacaatgaga gcatagaaaa
     2581 atttaaaata aattaatcta taattttaaa tttaaactaa agctataaga taagaatgta
     2641 attataatag taataaagac tgtatataca ttgtgcatat aaatgcgaaa tgaggtatat
     2701 acagtctttt tataattaat tgtaaaatga tttatgttat atgaaaaatt tacatatatt
     2761 aaggctacag ttaattgtca agagctggag tattcatttg taatacacaa tataagctta
     2821 attactatat tttattgtgt gttttttgtg gatttttatg tcaataaatg atatagtata
     2881 gataattaaa tgtattaaat atatttaatt tttttatttt ttgagtttta taagtttatt
     2941 tttatatatt acttatatat tacttatata ttatttatat tttgggttag atacactttg
     3001 acatattttc aaaagttaaa agattaaaaa taaagtagaa atttatgtta agtaaggaga
     3061 ttaatatgag aagaaataca aaattattaa caacagggat tctttcaatg gcaatcgtcg
     3121 cacctacaat ggcatttgct actgaatcta atgctatgga aaataacgct gatttaaata
     3181 taaacttaga gaaaaaaagt atcgttttag gtagcaaatc aaaagttagt gtcaaattta
     3241 aagaaaaacc agatgcagat agcattacat taaagtataa atgctatgac atgccattgg
     3301 atacaactct aaattacaat caatcaactg gggcatatga aggaactatc aattataacc
     3361 aagacccaga atatctaaat gtttgggaac tacaagggat aacaataaac agcaaaaata
     3421 atcctaaaac tttaaacaga caagacctag aaaagctggg attaaattta aaagactata
     3481 atgtaacaca ggaatgtata attgaagaca taacttctag aaaagatgta aataaatatt
     3541 tgagaaaaac ttcttcacct attacagaac ttacaggaag tgatagatat gaaacagcag
     3601 ttaaaataag taaagagggt tggaaaaatg gttcagataa agtagttata ataaatgggg
     3661 atgtaagtat agatggcatt atatcaactc cactggcaac cacatataat gctccaatac
     3721 ttttggttga aaaaaacaat gtgcctaata gtgtaaaatc agaattaaag cgcctaaacc
     3781 ctaaagatat aattataatt ggagatgaga atgctatttc taaaactact gctaatcaaa
     3841 ttaaatcaac tgtaaatgct agtcaaacac gtttaaatgg ttctaataga tatgagacat
     3901 ctttattgat agcaaaggaa atagataaaa atcatgatgt ggaaaaaata tacataacaa
     3961 atgctaatgg cggagaagtg gatgcactta ctatagcagc aaaagcaggt caagacaagc
     4021 aaccaattat attaactgat aaagatagta ttacagacaa tacatataaa tggttaaaga
     4081 gtgaggattt acaaaatgct tattttatag gtggtcctca aatgatatca acaaatgtta
     4141 taaataaggt aaatggaata actaaggata gtgttactaa taatagagta tacggagcag
     4201 atagacacga aacaaatgca aacgtaataa aaaaattcta tacagatgat gagttagagg
     4261 ctgttttagt agctaaatca gatgtacttg ttgatgcttt agcagcaggt ccattggctg
     4321 cgaacttaaa atctccaata cttataacac caaagacgta tgtatctgca taccataaag
     4381 ataatttaga agctaaatca gctaataaag tatacaaaat aggaggagga ttgacttcta
     4441 aggtaatgag ctctatagca tcatcattat ctaaacacaa tacgactcca acagaaccag
     4501 gaaatagtgg gggcaagaca gttatgattg acccagggca tggtggttca gcacctggaa
     4561 attcatctgg aggaatgatt gaaaaagatt acaatttaaa tacttcactt gcaacaactg
     4621 aatatttacg ttcaaaggga ttcaatgtaa taatgacaag agacacagat aagactttat
     4681 ctcttggaaa tagaactgct ctatctaatt cattgaaacc agatttattt acaagtatac
     4741 attataatgg ctcaactaat aaacaaggtc atggtgtaga agtattttat aagcttaaag
     4801 ataaaaatgg agggactact aaaactgtag ctaccaatat attaaataga attttagaga
     4861 aatttaaact tacaaataga ggtataaaaa caagagtact tcctagtgat tctacaaaag
     4921 attatttata cgttttaaga agtaatgata tgccagctgt acttgtagaa tgtgcatttt
     4981 tggataatga aaatgatatg agtttaataa actcatctgc aaaagtaaaa gaaatgggta
     5041 cacaaatagg taaaggaata gaagattcat taaaataaat ggaaataaaa atatttaaaa
     5101 acttaatgta aatttatagt tgtaaattta tactaaagtc aaaaaatgaa atataagtgt
     5161 atcaatagta agactgtatg tacatttgca catgtgaatg caagtatata tacagtcttt
     5221 ttttgtagtt aaatgcaata atttgattgt gttttgtaaa atacacaata tgatataatt
     5281 agataatata cacttaattt aattgaggaa ggtgattata tggaatattt atttgaaaaa
     5341 agtagtttaa aagaatatgg aaatgaagct ttagataaat atagagaatt attagataaa
     5401 actggtaata attcaaggtg tattcttttg ttagtaccaa acaataacac aaagatacga
     5461 tatgaaaggg ctctaagatt aaattatagt gaagaactga aagtaacaac atatataagt
     5521 tttgtgaaga aagaattagt taaatactgg ccactaataa tagaaaaatg tgatggaata
     5581 agtacaaagg ttgtatctcc aacatttata tcaaatagtt taagtgaata tttaatagtg
     5641 caaaaagtta aggaaaaaag gatacaagaa ggatattttg aagatattac aggaaccaat
     5701 aaatccatag caaatagtat aatgactaac ataaataagt ctgcatttaa tttaattgat
     5761 tttgatttta ttggagaaaa aatatattca tcaaagaaaa ataaagatag tatatataga
     5821 ttttcataca ctcaaatgga tgaaattata tcatattata taaattcctt actatcaaat
     5881 tcaattttag ataatgcatt aagcatatat ttatacaatc aatacctact taatgatgat
     5941 ttttatttaa aaaacttatt tacagaagtg agatatataa ttgtggatag ccttgaaagc
     6001 tcaagtaatg cagaagttga ttttataaca gaagcattgg ataatacatt acaaagttat
     6061 atatattttg attatagtag ggattattct gtatttaata atgtggatat agactatata
     6121 aatgagaaaa taatatcaaa aattaagcct aaagaagaaa atattaaaaa gaaggagatt
     6181 agaatagaag atttatactt attacctgca aatatagaat taaatcaatc aagtcaatta
     6241 tataatgaaa tgttagattt aataagtgaa aaagttatat ctcttataga agaagatgta
     6301 tctccaagag atatagcaat tatatctcct ataaacaata gtatattgga gcatcaaatt
     6361 agagattctt tgatagaaaa aaatatagat gtttttagta ctaagaagga taaaaaagct
     6421 gtggattatc catatgggaa tgcattggta gtggctacat gtatattcta tggatattta
     6481 gattttataa aagatgatga atacttaagc tttttagaaa ctcttttaga aataaatagg
     6541 ataaaagcat ttaagatttt taatgaaaca agacatttaa gtattgataa tatagatgaa
     6601 catagtcagt atcgtgatat tttacagtat attgaagaaa aaaagaagag tgatataaaa
     6661 atacatgaat ttttaactca attttacata gataaaatgt taaatttaaa agaaggtaaa
     6721 aagaatgttg ggttatgtaa aaagataata gctgaaagtg agtcttttag tgaaagttta
     6781 agtctacttg ggatgaaaaa agaaaagata tttgtagaag ctttaaaaag tacaataaac
     6841 gattattact ctgttgtcga tatagaagag ttgaaatcca aagacaaaat agtaataact
     6901 actccttatt catatatatc atcaaatata gatagaagta tacaaatatg ggtagatata
     6961 ggaagtaatg cttggaatat gaaaatagaa aaagatataa gtaatttaat tgtacttaga
     7021 aaatcttttg aagaaaaaaa gatttataca aatgaaatgg aagaatatta taaaaaatat
     7081 tatctataca atacagtata taatttgtta ttaaatgcaa agaaaatcta tgcatttaaa
     7141 agtgaatatg cgattaatgg ttatatccaa gagagtatat tatatagtat acttttaaaa
     7201 atctcacata aaggagataa aaattatgat taatataaat tatagagaag accagatacc
     7261 aataataaat tacgataatg gtactatggc agtaccagca gtaccaggtg ctggaaaaac
     7321 atttataata acaaatttag tagctaaact attacttgaa cagaaacata agagtggtaa
     7381 aatattaatt cttacatata tgaatagtgc tgtaaataat tttaaaggta gaataagaaa
     7441 gattcttgaa gagaataaaa tagatgatac taattcgtat gaagtaatga ctatacacag
     7501 tttggctgta aaaataataa aggaaaagcc agaagttgta atgttaagtg aggattttaa
     7561 catagctgac gatttgcaaa aaagtattat attaaatgaa tgtataagta gatttagatt
     7621 tgaaggtgga gaaagagctt ttagatggtt tttaaaggag caaaaagatg aacgctggaa
     7681 agaaataact cttgaagcct gggaaagagg tttttttgaa tttgcaggaa atgcaataag
     7741 tgaacttaag tataagggaa tttctccaga taaacttgaa gaaatattgt caagaggaca
     7801 caaaggaatg cttaaaatta ttttaccaat atataaattg tatgatagaa aattaaagca
     7861 aaatggattg ctagattatg atgatatatt aattttagca gaaaaaactt taagcttaga
     7921 cgaagggctt agaaagaagt ttcaaactag atacaaatat atatttgaag atgagtgtca
     7981 ggactcaaat gaaattcaag gtaatataat aaaaattata tctagcgaaa ataaaaacct
     8041 agttagagtt ggagatataa atcagagtat aacaggaaca ttttcatcat cagaccctaa
     8101 attttttaaa gaatttataa atagtgcaga tttctgttat aggatggata tgtcaaatcg
     8161 aagttcaaaa gatatacttg atttagcaaa cactcttgtc aagtatgtta caaaggaatt
     8221 tagacaagaa gaatgtagac atgctcttga agatatgcaa ataaaaactg tgccaaatgg
     8281 aatgggatat aaagaaaacc ctaatccaga acattataat ataaatgtaa agtggtatga
     8341 gagttggaaa attgaaatag agcaaactgc tagatacgtt aagggtataa aaaataaata
     8401 tccagataaa agtataggta tattggttcc atttaatgaa caagtgacac aagttgctaa
     8461 agaacttaga gagaataatc taacctttga agaattagga ccaaattctt tgaataaaag
     8521 gaaggttata aacaacatag catatattat tgattttatg ttacattgtg atgatgtaga
     8581 aaagttaata gttgtactta ataaagtttt tatagatact gataatgaag aagaaataaa
     8641 gtatttttta gaagaattaa aaaactattc taccgaagaa attatttata attttgaatt
     8701 tagtaagtta gataatgatt taatagaatt taaagaagaa agtgatatat acttaagttt
     8761 taaaagagga atagatatta taaaacagat attagaatat ccaataataa ggttagacct
     8821 cttagttttg tttattggga aaaaacttag tttagaaaaa gaagataaag ctgtactgga
     8881 ttatatatct ttttatataa agtatctagt atcagaaaat ataaatatgg atttagaaga
     8941 tgtatataat ttattgtttg atgtaaagaa cagggtgttt aatcatataa tagatgtggt
     9001 atatgagata aatggatatg aaccagagcc tggtagtata acactatgta actatcataa
     9061 atcaaagggg atggaatggg attgtgtgtt tttactaggt cttgttgaat ataatttccc
     9121 tgacaatatt aatcaaaagt ttcagagtga taaatggtat ctaaaagaaa aatataaaaa
     9181 ccctatggca attattaaat cagaagttga agctatatta aaaggaagta tttcaacaga
     9241 ttatgctcat aaaacgaaga ttgattctat aaatgaaaag attagacttt tatatgtagg
     9301 gattactaga gcaaaggaaa tgttagtact ttcaggaagt gcttatagag atgaaagtga
     9361 tatagggaat aagagaaaag agcaaaaacc atgtatatat ttaagcagac ttaatcaaca
     9421 tattattgaa aaaaggtcaa attaatatat aataagttat taaaagattg tttgaaatat
     9481 atctaaaaga ggataaaaca atatgtacag taagtaacta ctaaaaaata aagttaagag
     9541 agaagtgata tgagtgaacg ataaattaaa atatttttct tatagtcaaa attcaattaa
     9601 cacatataaa tcatgtccat tgaaatttaa atataaatat attgatagaa taaattggaa
     9661 aaatgatgat gttggtagta gagagtatta tgaaacttta aaaacaggaa gagattttca
     9721 tttgatttgt gaaagatatt ttagcaatat acctcttgga atctatttta atgaggatga
     9781 taaaaatagt aaaaagtttt taaagtggat ggaaaatata aaaaaagttg ttcctattga
     9841 aaaaggaaaa acgtatttgc cagaatatga agtaaggatg acattaaatg gggatataat
     9901 acaggcaaag tatgatttag ttgtaattgg aaataacaat atagaaatat gggattggaa
     9961 aaccgagtct aaaaagattg actataaaca cgtagaaaat agaatacaaa ctattgttta
    10021 tatgttttta gcaaaagaag taatacctaa agttttaaaa atggacataa atgttaaaga
    10081 tataaatatg agatattatc aacctgaatt tgatgactta ccaattacca ttttatatga
    10141 tgaagaaaaa catgaagcaa atagaaataa aattcaaaat tatataagta tgataaaaaa
    10201 cactaattat gaagaacata catacgaaga caatctatac aatgatatag aaaaggtata
    10261 taataaagaa gaacgtatgt gctatagaaa taaaaagcat tgcaagtatt gtgaatttaa
    10321 taagttatgt aatggcaaag aaatagatta tagtattttg gaggcggaaa tttatgggac
    10381 ttaggtttgt attaggtaga agtggaagtg gaaaatccac ctatatacta gatgagatta
    10441 aaaaagaagc tcaaaaaaat gaaacgacat caattatact tttagttcca gaacagtaca
    10501 catttgaggc tgaaaataga gtaagtaaac tatttttagg aaaagaaaaa gataagtatc
    10561 taagagttag ggtacttagc tttaagacac taagtaatat agttttttca caagtaggag
    10621 gattgacaga tgtaaatata aattctagtg gaaaagcaat gatggtatat agagctattg
    10681 aagatgtaag tgaagaatta aatgtatttt caaaatcaaa atcgcagtct ggttttgtaa
    10741 gctcaattac agatatgata tcagaaatga aacaatacaa tataagccct gaaatgttag
    10801 aaaatatatc tggagaattg gataatgaaa ctttaagttt aaaattaaaa gatataagta
    10861 aaatatataa ttcttttgaa gggaaattgc atgaaaatta tgtagatgct caagatatgt
    10921 taacttcatt ggcaagtaaa attgaattaa gcagttattt agatggagct tgtgtttata
    10981 tagatgaatt tacaggtttt acaccaaatc agtacaatgt cataaagagt atattaaata
    11041 agtctaaaag tgttaatatc tctttgactg tagatgatat caactatatt gggtacagta
    11101 aatctgatat gttttccaga acgaaattta cttattcaaa attgactcaa ttgtgcaacg
    11161 aagaaggcat aaaaatattg ccacaagtaa atttaaacac aggtgttata aaaagatttg
    11221 aaaaagttaa ggaattacag cacttagaaa gattttataa tgcttatcca tataaaatat
    11281 attcaaatca aactgaaaat ataaagataa aagagtttaa caatttatat tcagaggtgg
    11341 aagagatagc cagagaaatt gtacatttag ttagagataa gaatgtcaga tatagagata
    11401 taaccattgc tacaagagat ttaaatagat atgatttttt agtacactct atatttaatg
    11461 aatataatat accaaacttt atagataaga agagagaggc aaaaagtaat ccaatagtta
    11521 ttttaattat ttcagcttta gaaatgaaga atagacgata tgggtatgaa actatgttta
    11581 ggtatttaaa aagtggactt ataggaattg ataatgatga tataaattta ctagaaaatt
    11641 atgtgttggc taatgggatt aaaggtaaaa aatggtttga tgaaaaatgg gattatagaa
    11701 taacccaaag tctttcaggt caagaaagtg aatttgaact ggaattaaaa gagaaaataa
    11761 atgaaataaa aaatagagta ttagagccta tagtaatttt acaagaaaaa ttaagaggaa
    11821 aaaatagagt aaaagaaatc tgtagatata tatatgaatt tttattagat ataaatatgc
    11881 cagaaactat agaaagttta ataataaatt ttaaggataa aggggaactt gatgttgcaa
    11941 atcaatactc acaagtttgg gacatagtag tggacatatt agaccaaatg gtggaactta
    12001 tgggtgatga aataatttct ttagaaaaat ttataaaatt aataacctta ggttttgatg
    12061 aatatgaatt aggactggtt cctcctagta tagaccaggt acttgtaagt agtgtggata
    12121 gaatgaaaaa tccagacacg aaatatttat acctagttgg aactactgat ggtgtgtttc
    12181 cgctgattac aaaggatagt ggaatattga gtgataatga tagggaaagt cttggaaaca
    12241 aaggaatcga agttgatatt gatagtaaga ctaggagttt tgaagaacag tttcttgtat
    12301 ataaagctct tacttcgaca tctaagaatt taaccataac atatccaata tcagaccatg
    12361 aaggtaaaac gcttagacca tcaataatta tatcaagact taagaagata tttccaaata
    12421 tagaaaataa aagttattta gtggaagaaa ataaaagcac tgataaagat atacttaaaa
    12481 aaataacggt taaatcccct acatttaatg aacttataaa cgtaataaaa aattatgaca
    12541 gtgatggtta caatacagaa gagattaata gtatttggtt agatatatat aggtactatt
    12601 taaaagatga aatctacagt tctataacaa aaaaagttat aaaaggtctt agttatacta
    12661 atcaagttca taagattgaa gagaaaaaaa taaggtcatt gtataaaagt aattctctta
    12721 gcatatctag gcttgaaaaa tatgctgaat gtccatttgc ttattttata caatatggtc
    12781 taaaagcaaa aaagcgtaaa gagtattcat ttacaccacc agatttagga acttttattc
    12841 ataatatttt agataggttt tcaaaagaac ttttacaaga taatttaact tggagagata
    12901 ttgatgaaaa atatatagaa ttaaagatag gtataatagt tgatgaaata attttaaaga
    12961 taccaggata tatattaaat agttctgaga ggtataaata ccttgcatat agattaaaga
    13021 atatgttgac aactgctata actataatta gtcagcaaat aaaacaaggt tcatttgaac
    13081 caatagatta tgaagttaaa tttggcaaca atggaaaata tccacctata aaaatggtgt
    13141 tagaaaatgg acaagaagta agtttaattg gacaaataga tagagttgat gaatttgaag
    13201 aaggagaaaa taaatatata agaataatag attataagtc aggcaataag tccataagtc
    13261 taacagaaat atattatggt ctacaattac aattacttgt gtatttagat gcaatccttg
    13321 aaagtgcaaa ggatgaagat ataaatataa atccagctgc aatactatat tgtagaataa
    13381 ataatccaat agcaaaattt aatgaagata aagatgatga agaaatacaa gaagcaatat
    13441 taaaagaact tagaatgaag gggcttgtag taaaagactc tcacatagtt aaagagatgg
    13501 ataaatcatt aatagatgga gaaaggaaaa actctttagt aataccagta ggtcttacta
    13561 aggatggaaa tgtaggtaaa agtacttcag caataagcta tgaggatttc aaactgctta
    13621 gaaaatatgt aaggcatgct ataaaggatt tatgtgaaga aatgttaagt ggagaaatac
    13681 gtatagctcc atataaacat aaagatggaa catcatgtga cttttgtgat tactctgcta
    13741 tatgtcagtt tgattcaact ataaaagata acaagtataa aaatttaaat aataagagta
    13801 atgaagagat aataaaaatg atgaaaggag atgttaatta gtgagttctc ctaaatggac
    13861 aaaagagcaa ttagaagtta tagaaagtag agaatgcaat ttattggttg ctgctgctgc
    13921 tggttcagga aaaacagccg ttctagttga gcgtataatt caaatgataa caagtagaga
    13981 aaatccaatt gatatagata agttattagt tgttacattt actaatgctg ctgcgtcaga
    14041 aatgagagag aggatagggg atgctatagg aaaagcctta gatgaaaacc cagaaaataa
    14101 acatttacaa aaccaacttg ttttattaaa taaatctagt attacaacaa tacactcatt
    14161 ctgtttggat gttataaaat caaactttca tagaataaac ttagacccta atttcagaat
    14221 aggagaccaa actgaatgtg ctattttgaa acaagaagca atagaggaag tatttgaaga
    14281 tttatatgaa gaaagagatg aggggttttt aaatttagtt gaaagttatg cagaaagagg
    14341 aggagataaa gaagtacaag atattatctt gggaatatat tcatttgcaa tggcttctcc
    14401 tgaacctaaa aaatggttga ttgactcagc agaacgattt aatattgatg aaaattttga
    14461 tttttcacag tctatttggg ctagagctat attagatact gtaaaaatag agattaatgg
    14521 tctttgtcta aatatggaaa gggcattaaa agaagtggaa tcaatagaag aattagaaac
    14581 ttttgcagaa aagttatccg ttgaatataa aaaaattgct gacatttcac aagcatgtaa
    14641 taaatcttgg gatgaggcat ataaaaagat ggcaagtatg tcctttgaaa attatgtaaa
    14701 aggagtaaaa agaatttcaa aagatgctcc ttcgtatata aaagaatcaa aagaaaaggc
    14761 aaaaactata agagataaga ctaagaagtc tttagaaagt atagtaagtg ctacattcaa
    14821 taaagataat gattctatta gagaagaaat aaaatatctt tataacatag ttaaaccaat
    14881 atctagtgtt gtactgagat ttgaagagga atatagcaat aagaaaagag aaaaaggaat
    14941 aatagatttt aacgatatag agcattttgc attaaatata cttacagatg tagatgaaaa
    15001 aggtaatatt gtaccttctg atatagcagt tggatataga aacaagtttt atgaaatatt
    15061 tatagatgaa tatcaggata gtaatctagt gcaggaagta ctgcttaaag ctgttgcaaa
    15121 tacagaaact ccaaatagat ttatggttgg tgatgtaaaa cagagtatat atagatttag
    15181 acaagcaaag ccagaattat ttttgcaaaa atataataac tataatgata aaaagggaag
    15241 ctcccataga aaaataatgc tttacaaaaa ctttagaagt agagaagaag ttgtagatgc
    15301 tgtaaattat atatttgaaa atataatgaa tgagaatata ggagaaattg aatatacaga
    15361 aaaagaaaga ctaaatttag gtgcaaattt taatgtggat acagacgaga aatctattat
    15421 tggtggagca acggagatac acctaataca aaaagataat aaacttgatg atgatattat
    15481 aaatgataaa gacgatagaa ttaataacaa agaaaatgaa atagaggaag aagaaaaatt
    15541 agataatata caacttgagg ctagaatggt tggaaacatt atcaaagatt taatgaaagt
    15601 caatgaagat ggaaaaattc aaaaggtcta tgataaggga atagatggat atagacctgt
    15661 ggaatttaga gatatagtta ttcttttaag agctacatct gcatgggctc cagtgtttgc
    15721 agatgaattg atgaatatgg atataccaac gtatgctgat gttggagtgg ggtattttga
    15781 tacgattgaa ataaaaacaa tattgtcatt acttcaaata atagataatc caatgcaaga
    15841 tatacctctt atatctgtat taaaatcacc tatatttgga tttacaccag aggatttgat
    15901 tgatattaga gttcagagta aagataaaat tttttatgag gttttaaaaa gtacagcaga
    15961 gtatgatgga tttacagatt ctcaaaatga aaatgagtct gagtttatac caagtgaaga
    16021 atgcataaac aaaagtaaag attttttaat taagctaaag gaatttaaag aaaagtcaat
    16081 gtacatgagt acagatgaat ttatatggta cttgtacaca aggactggat actatgccta
    16141 tgttggagca ttgccaggtg gaagtcaaag acaagctaat ttaaaagtat tgtttgagag
    16201 agcaaaacaa tttgaagaaa ccagtcttaa aggaatattc aactttgtaa attttataga
    16261 gaaattaaaa aaatctagtt ctgatatggg aagtgcaaaa acacttggtg agaatgcaaa
    16321 tgttgttaga ataatgagta ttcataaaag taaaggtcta gaatttccag tagtcatatg
    16381 ctctgcaatg ggtaaaaatt tcaatactca agattttaaa aagagtattt tatatcatca
    16441 taatttaggg tatggacctc aatttgtaga ctatgagaga aggatttctt ttccaagtat
    16501 agccaaagaa gcattaaaaa gcaaaataaa tatagaaaat ttatcagaag agatgagagt
    16561 tttatatgtt gcatttacaa gagctaaaga aaaattgatt ataactggtt ctactagaaa
    16621 tatacaagat agtataaaga gatggtcaaa tggaattgaa agtttagata caatatcaca
    16681 atacgagata ttaaaaggta aaaatttttt agattggata atgccttgtg tattaagaca
    16741 tagggattta tctaatctac tagaagaagt aggattagac gcagttttta atgttgaaca
    16801 taattcaaaa tggtatggaa agttgtggaa taagagtgat attttagtgg aaaagaagag
    16861 tgatgaagaa aaagaaagta ttgaagaaat tcttgaaaaa atagatgtag ataatcctga
    16921 tagtgattat tacagtgaaa tagaagaaaa attaaactat atatatccat atgaatttag
    16981 tacaagaaaa cctgctacta tatctgttac agaaataaaa aagatacaaa ataattatga
    17041 agaagagtta ataaatacta tatttgaaca aaaagtaata ttaaaaaagc ctctatttat
    17101 tcaaaatgaa gaagaaagag aaaaaataag tggaacagaa agaggtacaa tagtccattt
    17161 agttatggag gttttagact taaaaaatgt aagtagtgta aatgatataa aatcacaaat
    17221 aagaggtttt gtttcaaaag gtataataac agaaaaacaa gctagtatag taaatccata
    17281 taaaatatat aaattttttg cctctaacat aggaaaaaga atgttgaatg ctgaaattat
    17341 aaatagagaa aaatcaattt atgcacaagt aaatatgaaa gacatatata tttatgaaaa
    17401 gcttataaac aatgacgata aaaaacttta tgataatgaa agtgtcatgt taagaggtat
    17461 agttgatgct tattttgaag aagacaatca aatagttttg gtggattata aaacagattt
    17521 tgtaaatgag gaaaatataa atcaaataat agagaaatat aaaaaacagt tggatttata
    17581 tgcagatatt attgaaactt taactggtaa gtcagtaaaa gagaaatgta tatatttatt
    17641 tggagttgat gaagctgttt gttattagtt tttatatata aattaaacct aaaagataga
    17701 ggaaggggaa aaaatgagat ttattcatac atcagattgg catctaggga agtccctaga
    17761 aggccattct agaattgagg aacaagcaaa gttttgtgag gaatttataa aaatagtaga
    17821 atcaaatgaa atagatatgg ttataattgc tggagatgta tatgatactt ctaatccacc
    17881 agcacaagca gaaaaactat tttatcagac tgtatcaaga cttgccaata atggtcagag
    17941 gtgtgtatta ataatttcag gaaatcatga taatccagag agactctctg caataacacc
    18001 tctagcacat gaacagggga tacttatata tggttatcca ttaagtgcta cgatagaagc
    18061 taagtataaa gggtttgaaa taacatatgc aactcaggga tgtacaaaac tgaatataaa
    18121 tggagaaaat attgtggttg ctacattacc ttatccaagt gaaaaaagat taaatgaagt
    18181 tttttcaagt gaagatgagt ttgagaaaca aaaaaattat tctgaaaagg taggagatat
    18241 atttagaagt ttagaagaaa attttagaag tgatactata aatatagccg tatcacatat
    18301 atttgtaata ggtggagaga gtactgaatc agaaagacct atacaattag gtggaagttt
    18361 tttagttgaa agaaaagatt tgccagaaaa ggctcaatat actgcactag gtcatttaca
    18421 taaacaacaa aaagcttccg aacgtttaaa tgcatactat tctggttctc cacttcaata
    18481 tagcaaggat gaaagagcgt ataccaaagg ggcatacata gtggatataa aagctggtga
    18541 aaaaccaata attgaagatg tatattttaa taattacaaa cctatagaag tatttaaatg
    18601 taatggaata gaagaagcac tagatatatg tgagcaaaat caagatagag agatttggtc
    18661 atattttgaa ataaatactg atgagataat atcacagaat gaaattaaaa aaatgaagga
    18721 actattaaaa gacataatag aaataaaacc aataataaca tcttgttatg aacaagaaag
    18781 tgtagatata aaagaaaagt ctatggcaga gttgtttaga gagttctatt cttttagtaa
    18841 aggtgtagag ccaaaaggag aacttatgga tttatttcta gatataataa gtgaagaagg
    18901 tgaatcagca gatgagacca attagattag aacttacagg gcttaatagc tatattgata
    18961 aacaagttat agactttgaa aaattaattg aaagaggttt gtttgggata tttggaacta
    19021 ctggaagtgg aaaatctaca atcttagatg caataactat tgctatgtat ggaaatatat
    19081 ccagaaatac aaaagaatat ataaacagtg tgtgcgataa ggcaattata tcatacgagt
    19141 ttgagatagg aagcaaaaac actagaagaa gatacattgt agatagaacc attgctagaa
    19201 gcaaaacagg aacaaagacc tctaatgcta gattaataga agtattgaat gacaataccc
    19261 aaaatgtttt agctgataaa gttgtggaag taaatgaaaa agtagcgcag gtcgtaggat
    19321 taacggcaaa tgattttact aggtcagtag ttttaccaca agacaaattt aatgagtttc
    19381 taagattatc acgtgcagat agaagagaca tgttagagag aatttttaat cttgagaaat
    19441 atggaagaag tcttggcgaa aaagtgaaaa agagaaaaaa tatacagtta caaaatttaa
    19501 aagatttgaa atcaaaatta agtcaatatg atggaattac agaggaagta tataacaata
    19561 taaaccaaga attaatagag ttaaaaaatt tggaaaaaga taagaataat gctttagacc
    19621 tagctcaaaa atcttatgaa gaaagtaaaa cagtatatga agaacaatta aagttagaaa
    19681 aaaatgaact tagaaaaaat gagcttgatt taaaaaatag tgagataaaa gaaaagattg
    19741 ctcttgtaga aaatgatagt aacgctaaaa aggtagaccc atacataagt tcagttcaaa
    19801 atctagaaaa aaagatagat gaagatagct tcgcagtaag taatttagaa aagaaattag
    19861 caatattaaa tcaggagctt gaagttacaa aaaatagata tgaaaaaata aacaaaatta
    19921 agaatgaaga aatccctaaa ttgagtgaag aaaaaataag attgcaacaa gcaataaaat
    19981 tagaagaaga gttagtttta ttagatagag agttaaaaga gctaaaagaa agtggaatta
    20041 atttaaataa gactaaagtt gaattagaaa aagtaaagca ggtgtcagag tccagaaaag
    20101 atgcagttac taaatctata aaagaagttg aaggaaagat tgataaagta aatataagtg
    20161 cagaattaaa acaaaaaata tttctagcat atgaatacga gaaagattac aacaacgtat
    20221 tggaagaaaa aaatcaaaag ctaaataaac tagaagaaat actcaaagag acagaaaata
    20281 taaatttaaa agttagatat attgataaag ataagaatga tgtaaatcgt aatttagaaa
    20341 atttatcact tcacttggat gttcttctta aaaaatgtcc tggtaagagt gctgatttac
    20401 ttttaaaatc tgaatatgtt actgaattaa gaaataaagc aaataatact aaagaaaatg
    20461 aaattaagaa aagcagtata caagatgaat taaagataat acttgaaagt aagtttaata
    20521 ctgaaaggga agtaaattta ctaaatgaaa aacttgaaaa taatagaaag aacagagatg
    20581 acttagaaaa agaattagaa gaactaaagt atttaaattt agcctctgaa cttagacgtg
    20641 aattaaaaga aaatatgcct tgtccagttt gtgggtcaaa acatcatgaa aatcatgata
    20701 taactaagta tgatgaaaat atatcttttg tcaaagaaaa actggagaaa ttagaaaaag
    20761 aaaaaataag tataagaaac aatatagagg aattaaatgc taaagtaagt gggtatttat
    20821 ctatagaaaa aatgaagact aaagaacttg aagatgtaaa aggaaaacta ggggggattc
    20881 catctagtca attattaaaa aaacttgatg aagaacaaag aaaacttgca ttgttaaaat
    20941 ctaatattca ggagtgggag aaagaaaaag agagtactga aaataaagta acattagcta
    21001 aagaagaaaa aaataaaatt gaaaaagaag aattaaaaat aagagagagt ctgaataatt
    21061 ataagaaatt aacaagagat ttaaacatag aaatagaagg tttagaaaat aaatgtaaaa
    21121 aacttaaaca ggaataccta ggattaaaga cgattactaa agtctctaat ctattgtcaa
    21181 aagtaaaaga aataagggag aatgaaaaag aattagaatt actaagtact gggcattcaa
    21241 atttattaaa aaatagagac tcactagata ttaaaataag agaaaatgag agtaaactac
    21301 atgaaataga gatagaatta ataaaggcta gagaattata tgtagaaaag aaattgagta
    21361 gagataataa gtataaagaa gttattagca taacgaaagg tgatttagct aaaaatcttt
    21421 tacataatgt tgaagaaaat atatgtaaga tattagaaca agaagaatca agtaagaaaa
    21481 aattggaaga gcaaagatta gaatatgaaa aaaacattgc tgaaaagcac aatatagatg
    21541 gtagacttaa aactgctaag gaacaatata aagaccaaaa atcaacacta aataaactat
    21601 tggcagaaaa taaatttgaa agtatatatg cagtaaaaag agctttatta gatactgatg
    21661 cagtaaaaaa attgattgaa gaaatactag agcatgaaga agaacaaaag ttattatcat
    21721 ttaaaataaa atcatcaaaa gaaaaattaa atggaagaag cataaagaag gagtactttg
    21781 aacagttaaa agatgaaatt tataatttga aagtagaaat aggaaatata tccaaagata
    21841 ttggagctaa tcaaaatcag ttaataacat taaaagactc tttagataag ataaatgatt
    21901 ttaataaaca acttaaggta gttgaacaca atgtggactt attggaagaa ttagataaat
    21961 gtgtacaagg aaataaattt gtagaatatg tttctacaaa ccaattaaaa tatatagcgt
    22021 tggaagcatc aaaacgattg gatggaataa caaaaggaag atatgcactt gaaattgact
    22081 caacactaaa ctttgtaatg agagataact ttaatggagg agaaagaaga agcattgata
    22141 ctctatctgg tggagagaca ttcttgacat ctctatcatt agcacttgct ctttcatctc
    22201 aaatacaatt aaagggaagt gcaccattag aatttttctt tttagatgaa ggttttggtt
    22261 cattagatag tgaactttta gaaattgtta tagaatcctt ggaaagattg catagtaata
    22321 atctaagtgt gggtataata agtcatgtcg aggaacttaa aaatagagtt cctgttaagc
    22381 tattagtttc atctagtgag gctggaatag gttcaaaagt aaaaattgaa tatagttaga
    22441 aaatatatgt taattgaata tattatctaa tatttccaaa taatatgtaa ttgtgatata
    22501 ataaaaacta atattaaagt tatttataat taatattaat ataaaaacat gtaagtagga
    22561 gtgtggtaag tgttggaatc gcccaataat ttgattcaga ttattttttt aatagtatta
    22621 cttatagggt ctgcattttt ctcagcatct gaaacggcct taatgtcgtt gagtaaaatc
    22681 agaatcagat atatgcaaga tgaaggagtt aaaggagcta agttagtaag ttcattaata
    22741 gaaaatccaa ataagctttt aagttctata ttggttggaa ataacgtcgt aaatatagca
    22801 gcaacatcca tatcaacatc tttgtttata ggattaatgg gagaaaaagg agtggcactt
    22861 gctacagctg ttatgacagt attagtatta atatttggtg agattactcc aaaaacaata
    22921 gcagcaaata attcagaaaa ggtttctcta ttagtatcta aacctataaa agcaattata
    22981 tttatattaa gaccaatagt atggatattt aatataatta caaatattat atttaaatta
    23041 tttggtataa caaataaagg agctaaatca ttcataactg aagaagagct aaaaactatg
    23101 gtaaatgtaa gtcatgaaga aggcgtcctt gaaatggaag agagagaaat cataaataat
    23161 gtttttgaat ttggagatat gcaagctaaa aatgcaatgg tacaaagaat agatatggta
    23221 gctatagata tggaagatag ttatgatgag ataatacagg tatttaaaac tgaaaaatta
    23281 agtagaatgc ctgtgtatga agaaactata gatgatattg taggaatact taatataaaa
    23341 gatattatat ttttatctga tgaagaaata gagtcatttg atattaaaaa ttatatgaga
    23401 gaaccttttt tcacgtatga gttcaaaaag ataacacaac ttcttgaaga gatgaaactt
    23461 gaaaaaagtc agatggctat agttgtagat gagtatggtg gaacatctgg acttttaact
    23521 atagaagatt tagtagaagt cattgttgga gatatagagg atgaatatga tgaagaagag
    23581 gatgaaatac aagttataaa agaagatgag tatattgtag atggaagtac taaaattggt
    23641 gatgttaatg aacttattgg tgtaaacttg gaatctgagg agtttgattc cataggaggt
    23701 tttataattg gacacctaag cagattgcca gaagaaaatg aagtaattga agttgataat
    23761 ataagatttt gtatagaaag tatagaaaaa aatagaatta aaaaaataag aatatataca
    23821 taatgtaata atgttcttaa atttattaga agtgtataaa tataaaagat atcttttata
    23881 tttatacact tctttttatt ttgtattcaa ataaaactag tatgaaatgg acaaaataaa
    23941 aatataaatt agaagaaagg agatttttaa gatgagcata ctagaaaata attttttata
    24001 caaattaaaa aacaacacta tctttttttg tatatataca ttgttattta tttttattta
    24061 caaagcgttt ccatacatag ctccattttt tctaggtgct ataatagcac ttatgattaa
    24121 tcctatatca caaaagttag aaaataagtt tcatataaat aaaggtatct ctactcttgt
    24181 tctaagtttt cttgcagtgg ctatagtcag tacagttact acaataatag taataaattc
    24241 aatgaaagaa ttaatgggat ttttaaataa tatatctgct aatcctgagg atataagtaa
    24301 tactattatg tattttttaa ataaaataaa tgactttatg aaatcatttc aagaaatagc
    24361 caattttgat gtagaacaat tagtaaacaa attcagtggt gaagtaatgc aaattacaaa
    24421 aaatcttctt actagtatac ttggattggc tacttctatt ccatatatta taatatttat
    24481 aattaccttg tttattgcta cgtattttat agcaaaagac cttgataaga ttgaaaatag
    24541 tttttataat atgtttactg ttgatgttag aaagaaggta aaaaacgtaa agaaagaggc
    24601 agggctttca cttgttggat atataagggc ctatacaata cttatggcta taacattttt
    24661 tgcaatctgg ggaagttttg ctttatttgg attgaaatac ggtctaatag tagggtttgt
    24721 aggagcttta atggatttaa ttccattcct aggtataata acaatatatc ttccagtaat
    24781 tgtttattat tttcttataa aaaattattt tatagctata agtatgactg tgatattttt
    24841 tgttttgtct ttgattagag aaatacttga acctaaacta gtatcagtaa atgttggact
    24901 aaatccatta gctaccctag cagcgatttt tattggaatc caagtaaaag gaataattgg
    24961 agtaattttt tgtttgggtt tggtatgtat gcatgatata cttaagaagg tggatatttt
    25021 ttaattataa taattgatag attttttaaa aataaaattt tattcacagg taaataaaaa
    25081 aatattaatc ttattaagaa agagggtata tacgtgaaac aaaaagtagt ggaaagattt
    25141 ttaaaatatg tatcatttga tacaacatct aattctcagt gtgagaactg tccatcttca
    25201 gaaggtcaaa gagttttagc taagtatata gttgaagagc taaaaacaat gggtgttgat
    25261 gatgtgagtt tagatgaaaa tagttatata atggctactt taaaaggaaa tactgatggt
    25321 gtggatacta taggattcat atcacattta gatacaattg aagatgttag tggaaaagat
    25381 attaaaccaa aaataataga aaattatgac ggaaaagaca tagttttaaa tgaagcgtta
    25441 aatgttatta cgtatgtaaa ggattctcca gagttggaag aatttaaagg tgacgattta
    25501 atagtcactg atggaacaac tctacttggg tctgatgata aagctggtat agctgagata
    25561 gtgactgcaa tagagtatct aataaatcat ccagaaatta aacatggaga tataaaaata
    25621 ggatttacac cagatgaaga aattggtaga ggtgctgatt tatttgatgt tgaaaaattt
    25681 ggagcaaaat atgcatatac tttagatgga ggaatagttg gagaacttga atgtgaaaat
    25741 tttaatgctg caaatgcaac tattactata catggtagaa atgttcatcc aggttcagct
    25801 aaaaataaaa tggtaaatgc tatacatata gctgctgaaa tatcagaaat gttcccagca
    25861 gatgaaagac cagaaacaac agaaggatat gaaggatttt ggcatttaaa ttctataggt
    25921 ggaaatgtcg agaatgtaag tatggcttat attattagag accattgtaa agaaaaattt
    25981 gaaaatagaa aatctattat gatagagaat atagaaaaaa taaataagaa atacgataat
    26041 agagtggaat tggatttaaa agactcttat tacaatatga aagaaaagat tgagccagtt
    26101 atgtttatag ttgatatagc taaagaagct atggaggaat taggtataaa accacgttta
    26161 gttcctgtaa gaggtggtac tgatggagca agattatctt ttaatggatt accttgtcca
    26221 aatatattta ctggtggatt aaacttccat gggaaaaatg agtgtatacc agttagttct
    26281 atggagaaag ctactaaact aattgtaaga atagctgaaa aatatgctga aagagtttag
    26341 ttaaaaagta tttaaaaatt aagcatttaa agttataata gctatatcat tctttataag
    26401 tcaagctgat tataagcttg tgttgtactt aaaatggtat agctattttt gtaattattt
    26461 ttataaaaga taattatttt tagtttaggg tatcaataaa aagtatgaat atcaataggt
    26521 attaacattt aaaaggggga attgtattga aaattaaatc taaaaaaata atagcatttg
    26581 cactttcact tgggattatg ttgccaagtt gtgtatatgc agacaatatt agtaaaaatc
    26641 aaaatgcaca ggtagatatg aacaattatc ttcaaggtag aaaatctcca aaggaggaaa
    26701 aacttattgg tgaagataga tttgatacag cgataaaaat atctcaaagt ggttggaata
    26761 atggttcaga aagggtgttt ctagttaact caaattcatt gccagatgca ttggcttcaa
    26821 ctcctttggc aagtaaattg gatgcaccaa tactgcttac aaataaaaat agtataccat
    26881 ataatgtaac agaggaaata aaaaggctta atccagcaga agttatttta attggaagtg
    26941 aaggagctat ttcatcaagt gtaaaagaat ctcttgaaga tatggaagta ttagtcagta
    27001 gaatcggtgg agcagataga gaagaaacat ctttacttct tactagacag ctagatgata
    27061 ctggtgattt aggtgtctca aaagtggcag tggttaatgg gtataatgga ctggcagatg
    27121 caacttcaat atcaagccca gctgcaagtg ataatacagc tataatatat acaggtaagg
    27181 atagcataag aagtgaggca aagaatttta ttacacaaaa ctcaactaag acatacatag
    27241 taggtgggga gtacagtata agtaaaaaac ttgaaggaca gttagtaaac tcagaaagac
    27301 tagcagggac agatagaaaa gatactaatg cgaaagtttt agaaaagttt tatggaaaat
    27361 caagtaaggt taataatatg tattttgcaa aagatggaag tggtagagaa gctgatttag
    27421 ttgatgggct tgctgctgga gtattagcat caaaaacaaa gtcacctgta gtacttgcaa
    27481 gtggaagttt aagtagtgca cagaaaagtt ttataaaaaa agtaaaggct gataaatttg
    27541 tacaagttgg tggaggtaaa aatagtaaac catatgcaga agcattagcc ttacagtaat
    27601 attgtgtaaa tcaaaattaa tatataataa tagcaagtaa accaagttgc actttaatat
    27661 atataataaa ataagtaaac taagttatat ttcagtataa tatataataa aataagtaaa
    27721 ctaagttata tttcagtata atatataatg aaataagtaa actaagttat atttcagtat
    27781 aatatataat gaaataagta aactaaatta tactccaata taatatataa tagaaacaag
    27841 taaactaagt tatatttcaa tatagcatat aataaaacaa gtaaaccaga ttctatttaa
    27901 ttagaagttt aaggtattgt acttaaactt ctaattgaga tagaaataaa atggtttact
    27961 tgtctttatt atgaatttaa taaattaaag cttattttat ttttattaaa aatttactaa
    28021 tcattgatat ttattatatt tccagtattg atgtcaatta agtttatatc aagttcaagc
    28081 tcatcctcat catcagtttt tatgatatct ataattgcga cagaatggct tccatctttt
    28141 gggatagaag tacttcctgg atttattact attaaattat catccatgta tagttttttt
    28201 acatgagtat gtcctaaaac taatatgtca ccacccattt tctttgcttt gtcaataatt
    28261 tcttcttctg attctatata accatgattc aatataattc taatttcacc aaattggctc
    28321 atgacataag gactctgaat aggatgttct ataaccatct ggtcaacatc tgcatcacag
    28381 ttccctttta caatcacaat gttttctaat ttatttaatg tctcaataaa ttttttagga
    28441 ttatatcctt ctggaatatc atttctaggt ccatgatata agacatctcc accatgtaat
    28501 aaaacatcac aatcagataa tacatttaac gctttttcaa agtaaagtaa gctaccatga
    28561 gtatcactca ttattcctat tttcatatta aaaatcctcc taagtacagt ctataaaaat
    28621 ttttcagata ctctatgcca aaactcatag tttgacattc taagtaattt tatttttgca
    28681 tcagatatac taacttttat attttcgatt tgtctaaatc tgtgctcaac tccatcaacg
    28741 acaatcaata cagaatcttc aaatctatat tctggtgcaa tctttataac agattcattt
    28801 gaacatatta tacttgatgt aaagcaccta tatgcattgg tatttatcgg atgtaaaggt
    28861 gtaatctgca taagttcaag atttatatca acaatactac ctccagcaga atagttatag
    28921 gctgttgaac ctgtggaagt agaaataatc ataccatctc cactaaaatt ttgtatatgt
    28981 ttattatcaa ggcttaaatt taggtgtata gtcctagact tgtctcctct tataacaacc
    29041 tcatttacag caagcatatt agaaccacta gtagttgtat atacttccgc attaagaagt
    29101 gacatttctt gaataatata atcttttttt gtataagctt caataaagct atcaatctta
    29161 tctggtaaaa tatcaggaaa aaaccccaga tgacctgtat ttattcccat tatgggaatc
    29221 tcaggaaaat cgaaatctct aactgttctt aaaaaagagc catcaccacc aattgatata
    29281 ataagctctg tgtctggata aatatctgaa tacacttcaa agccagcatt gataagtttt
    29341 ctagttaaga gttctttagt ctcaagggac ttatttaatt ggtttgtatt tatagttata
    29401 attcttttca tatattcacc tcttcatact ttgtattata acatagtttt ataattctta
    29461 atttataaat ttataaatga cctttgtagt gaatgtgtta aaatatgtat aggtgggtat
    29521 aaaaaaatgc ataagaggtg aagatgtgtt tagcataagg aatatcacaa aaaaattagg
    29581 aaaattcaag ttaaataata taaacttaga gttaaaggaa ggggatattg ttgggattat
    29641 aggaccaaat ggttcgggaa aaacgacttt aataaaaatt ataatgggga ttattgatgc
    29701 agatgaaggt gaaattgaac tttgtaatga aactattgaa aattctccaa taagctttaa
    29761 aaacaatatt ggttttgtat atgattcttt acaattttat ccgcatttaa aggttaagga
    29821 atttagaaaa atagtttctt tattttataa gaattttgat agagaaaggt ttgatgaata
    29881 ccttaataag tttgatatag aagaaaatat gcatattgaa aatttatcta aaggtcaatc
    29941 tgaaaagctt atgttatcaa gtgcacttag tcacaatgct aagttattga ttttggatga
    30001 accaactgct gggatagacc caattgtaag aactgaaata atgcagtact tacaagattt
    30061 tgtgaaaaat ggaagtagta gtgtaataat ttctactcat aatactgata atttaattaa
    30121 aattgctgat tatcttgttt ttataaatag aggaaatcag atttttactg ttaagaaaga
    30181 attaatagaa caagaatata agataattag ggcaaataaa gcagagttag aagcaattaa
    30241 agaaagcata gttggtgtga aagagtataa atattataat gaggcacttg ttaaagttgg
    30301 agatagcttg aaagtaaaaa gcctattgat agaaattgat aagcataagg tcaaaaatcc
    30361 tactatagaa gaactaatgt attattatgt aaatgaggtg agatgatgtt caatttaata
    30421 agaaaagatt taataatagg gattagttca gatggtatcc gtaatttaaa gtacatattg
    30481 ctattttttg tgttttattt tttccttaat tcaatttcat attatacagt aagtattgtt
    30541 gttagttatt tgatatttat aaatacgttt gagtgtgatt atgaaaatga ttcaaggatt
    30601 tttataagga gtatgccagt tagtatagaa gatatagttt actcaaaata tttattaggt
    30661 gtaggattaa taatttctgt aacaataatt gtgtccttac taagtaaatt gacatcacta
    30721 gtgttcttta gaaatatggt tttaaatgat gtattttttt ctgtaaatat atttttagca
    30781 attttgacga ttttactgcc tttaattttt aagtttggat atggaaaaat gaaagtttgt
    30841 ggttttatag taagtatttt aatttacttt gtatatggaa gtcttttgaa gatgattagt
    30901 atgattgtat atcaagtaaa gcattttaat tatagtaaag ttggaggagt atatctttca
    30961 aattatgtaa ctgatatagc taatactaag tatataaat
//
LOCUS       DAEMGG010000038        30922 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEMGG010000038 DAEMGG010000000
VERSION     DAEMGG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.038"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            253..1074
                     /locus_tag="KPK24_001619"
     CDS             253..1074
                     /locus_tag="KPK24_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2299297.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    1153..1364
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1451..2767
                     /gene="argH"
                     /locus_tag="KPK24_001620"
     CDS             1451..2767
                     /gene="argH"
                     /locus_tag="KPK24_001620"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG2299298.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            3016..3354
                     /locus_tag="KPK24_001621"
     CDS             3016..3354
                     /locus_tag="KPK24_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG2299299.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(3481..4770)
                     /locus_tag="KPK24_001622"
     CDS             complement(3481..4770)
                     /locus_tag="KPK24_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2299300.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            4850..5554
                     /locus_tag="KPK24_001623"
     CDS             4850..5554
                     /locus_tag="KPK24_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBG2299301.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQRNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            5646..6692
                     /gene="selD"
                     /locus_tag="KPK24_001624"
     CDS             5646..6692
                     /gene="selD"
                     /locus_tag="KPK24_001624"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5694..5696,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG2299302.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            6710..8122
                     /locus_tag="KPK24_001625"
     CDS             6710..8122
                     /locus_tag="KPK24_001625"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG2299303.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            8236..10119
                     /gene="selB"
                     /locus_tag="KPK24_001626"
     CDS             8236..10119
                     /gene="selB"
                     /locus_tag="KPK24_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG2299304.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            10224..12962
                     /locus_tag="KPK24_001627"
     CDS             10224..12962
                     /locus_tag="KPK24_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG2299305.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(13081..14025)
                     /gene="manA"
                     /locus_tag="KPK24_001628"
     CDS             complement(13081..14025)
                     /gene="manA"
                     /locus_tag="KPK24_001628"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG2299306.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            14198..15559
                     /locus_tag="KPK24_001629"
     CDS             14198..15559
                     /locus_tag="KPK24_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2299307.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            15647..17536
                     /locus_tag="KPK24_001630"
     CDS             15647..17536
                     /locus_tag="KPK24_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2299308.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            17551..18006
                     /locus_tag="KPK24_001631"
     CDS             17551..18006
                     /locus_tag="KPK24_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2299309.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            18065..18382
                     /locus_tag="KPK24_001632"
     CDS             18065..18382
                     /locus_tag="KPK24_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG2299310.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            18407..19669
                     /locus_tag="KPK24_001633"
     CDS             18407..19669
                     /locus_tag="KPK24_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG2299311.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            19774..20838
                     /locus_tag="KPK24_001634"
     CDS             19774..20838
                     /locus_tag="KPK24_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2299312.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            20892..22070
                     /locus_tag="KPK24_001635"
     CDS             20892..22070
                     /locus_tag="KPK24_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG2299313.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(22261..23319)
                     /locus_tag="KPK24_001636"
     CDS             complement(22261..23319)
                     /locus_tag="KPK24_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG2299314.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSKIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            23386..23757
                     /locus_tag="KPK24_001637"
     CDS             23386..23757
                     /locus_tag="KPK24_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG2299315.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            23794..24468
                     /locus_tag="KPK24_001638"
     CDS             23794..24468
                     /locus_tag="KPK24_001638"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG2299316.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            24717..25556
                     /locus_tag="KPK24_001639"
     CDS             24717..25556
                     /locus_tag="KPK24_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG2299317.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            25800..25896
                     /locus_tag="KPK24_001640"
     tRNA            25800..25896
                     /locus_tag="KPK24_001640"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25834..25836,aa:Sec,seq:tca)
     gene            26051..26758
                     /locus_tag="KPK24_001641"
     CDS             26051..26758
                     /locus_tag="KPK24_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG2299318.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(26900..27736)
                     /locus_tag="KPK24_001642"
     CDS             complement(26900..27736)
                     /locus_tag="KPK24_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2299319.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSKAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            27924..28829
                     /locus_tag="KPK24_001643"
     CDS             27924..28829
                     /locus_tag="KPK24_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG2299320.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            28838..30022
                     /locus_tag="KPK24_001644"
     CDS             28838..30022
                     /locus_tag="KPK24_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2299321.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            30137..>30922
                     /locus_tag="KPK24_001645"
     CDS             30137..>30922
                     /locus_tag="KPK24_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG2299322.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIM"
BASE COUNT        12387 a         3168 c         5282 g        10085 t
ORIGIN      
        1 tcactttatt taatatgatt atatatcata tattgtaaaa tttctagcat tataagacta
       61 taaattgaaa atattcagaa aaatactaca agaaacgatt tattttgctc ttaaaataag
      121 ttaaatgaat gttattaatt ttgtaaattg aaatagttta agtataaagt tatgatataa
      181 tctaataagg gtaattcttt agattatatt aaggaaatat aggtatacaa taaaaagtta
      241 tggagttgga ttatgggata taaattaatt gtaacagata tggatggaac tttattaggc
      301 aataatcata aagttacaga tgaaaataaa actgcactac aaaaagttat aaaaagcggt
      361 attaatgtaa ctttagcaac aggaagagcc ttcgattcag ctaaatgcaa tgtagatttt
      421 cttaaagagg acatgccaat aatagcttgt aatggttcat taataagaga gcaaaatgga
      481 aatataatat attcaaataa gattgataca agaacatgtt taaacatttt agatgtatta
      541 gataaatacg atatttacta ccaatgtaat agtatagatt ctatgctttc gaaaaaaata
      601 gaaggacgtg aagatagatt aagtgtattt ttaggctctg aaacagaagt aattgtaaaa
      661 gatgatttga gagaagaaat ctttaaaaaa gatatactaa aatttgtaat catagaagaa
      721 aaaaatccat ctattttaga tgaaataaga aaagaattga gaaaagttca aggaataaag
      781 ataacttctt catggccaaa caatattgaa gtcatgaatg aaggtgttga taaaggtaat
      841 gcagttaaaa ttttagctga aaaaatgaat atagacagag aagatataat tgcatttgga
      901 gacaattaca atgacataga aatgataaag tttgctggtc ttggggtagc tatgggaaat
      961 gcagaagaat tgattaaaca agaagctgat tatgttacag atacaaatca agacagtgga
     1021 gttgcaaagg caatttataa attcctagag ttaaaagaat cacttagctc ttaaaacagt
     1081 atataatagg aaaaaatagg ttgactattt tgtagatagt aattataata caaatagata
     1141 aaacataaaa atttagattc agtgaaaaag agagtagata tagagaaaat ctcagcgaat
     1201 tgggggatgg tgtgagccta atgataacac tttatattga aaagaacttt ggagaatact
     1261 acttgaaatt cagtatggta gtaggtaatc tcgccttaag agaatagagt gggtaaacat
     1321 tgtttaccaa ctagagtggt aacacggaat atttcgtctc ttggtctttt tgaccaagag
     1381 attttttagt ttcattgagt tacaagactt ataattaaaa tacaaaaaat taaaatatga
     1441 ggtgaaagtt atgaaattat ggggcggacg ctttagaaaa gcggaaaatc aacttatgga
     1501 agaatttaat aaatcttttg gctatgattg tgtactgtac aaaaaagata tcgaaggaag
     1561 tgtagcacat gttcatatgc aagtaaaatg tggactttta acagaagaag aaggaaagtc
     1621 tataacagaa ggtttaaaag ggatacttga agatgtagaa aatggaaagt tagtattgga
     1681 tggagagtat gaagatatac atagttttac tgagataaat ttaattcaaa gaattggaga
     1741 tgttggcaag aagcttcata cagcacgtag tagaaatgac caagttgcag ttgatatgag
     1801 attgtatgct aaagaaaaag caaatgattt ggtgggttta atatctgaat ttaaagctac
     1861 tataaaagat gttgctgaca aaaacccagt gatgatgcca ggctatactc atcttcaaag
     1921 agctcaagta gtgacattta agcatcactt aatggcatat tatagtatgt ttgatagaga
     1981 tgagaaaaga ataaaaaatg ctatagaaat attagatgaa tcacctttag gctgtggagc
     2041 tttagcagga actacgcatg atatagatag aagtataact tgtgaacaat taggatttaa
     2101 aaaagtagta gataatttta tggatggagt aagtgataga gattatttac tagagctaat
     2161 gagtgatttt tcaattataa tgatgcactt aagtagatta agtgaagagt tgatactttg
     2221 gagttctcaa gaatttggat ttgttgagat agatgattta tatacaactg gaagtagtat
     2281 aatgcctcag aagaaaaatc cagatggtgc agagcttata agaggtaaga ctggcagagt
     2341 atatggaaac ttatttggtc tttttacagt aatgaaagga ataccattag catataataa
     2401 agacatgcaa gaagataaag aaggtttttt cgatagtgta catactcttg aaatgtgtat
     2461 acaaataatg gatagaatga tagccacact aaaggtaaat gaggataaaa tgaaacaagc
     2521 agtaaaaaat ggttttttaa atgcaacaga agttgcggat tatttggtta aaaataatgt
     2581 tgcttttaga gatgcacatg gtatagtagg aagtatcgtt atatattgtg aagataataa
     2641 aaaagcaata gaagatttaa cattagaaga attacataag ttttcagatg cttttaagga
     2701 agatatttat gattttatcg actatgagag tatattaaat aagggaataa aaaagaattt
     2761 aaaataatgt atttattttt aaaagctgta aatttgaatg ttatcattta aatttacagc
     2821 tttttattgt gtaagatttt attatataaa agtaagaaac ttaaaagtag tagtatttaa
     2881 aataaataca aataaaaaat tgttattaaa atgataatta atatcaataa gtattgacaa
     2941 aattatttct aaatactatt atatataata taaataataa tgaaaatcaa tatcaaataa
     3001 atgggaggaa atctaatgaa cttattagtg attggtggtc atgaaagaat ggaaaaagat
     3061 tatatggtta tggccaagaa aaaaggttat aagacaaagg tatatactac catgtcatct
     3121 aaattaaata attctatagg aagaccagat gcagttgtca tacttacatc aactgtttca
     3181 cataagatgt ctagaacggt tgagtctcaa gctaaaaaac aagatatatt aatcgtaagg
     3241 cataaaaata gtagtaaggt agcatttaat gaatgtctag atatggttga tgaatgtctg
     3301 ggaaattgtt caaaatgtaa gcttaaagac aaacaaaaac aaaaaaatat ataggtataa
     3361 aaaatacata aacataaaat taaaagtact gagataatag ttataattat tatctcagta
     3421 cttttagatt tataaggata agtatattat aaatttttta ttataatttc caactacttg
     3481 ttactttttc atattttttt tacttctagc tttttttctt tttttcttct ttatatttga
     3541 agctattata aataaaacta aaagcactag aatacctgca ataatcttaa caaaagtcat
     3601 taaaatatta tttccagtaa aaaatggaag actgctatca agattatttt ttgcaattaa
     3661 gtctatactg ttttctagtt taccattatt atatacattt aaagtaccaa ccttagcccc
     3721 tttttttata ggcaaatcaa ttttatttaa ctctgttttg gtatcataat tgccttgaga
     3781 ttcatctttt tctaagaata tcctataact attttttggt tcataaatca attcatgttg
     3841 ttttgtaaac aatacttttt tagtatctgt gtatttttct ttatcaacaa tagtagcaca
     3901 cttaaagtta tcaaatccat aatcaatcag aatacgtgag tctaaatata aatcagcatt
     3961 agttgaatta aatacagcaa cgataactct tctgccatct tttacagcac tcgataaaag
     4021 acatttacca gcagcatcag tatatccagt ttttatacca tcaactatat catattttat
     4081 atttataggt tgacctttgt atgtaatctt atcatgtgat gtcaaaaact tattagtgtt
     4141 tacaaagtat ctctcataag gatatgcttt tgtagcttca aatttaagtg acttagtttt
     4201 tacaatttgt ctaaaagtat cattattcat agcttctcta gctatgagag ccatatcatg
     4261 agctgttgtg tagtgatttg ggtctggtaa tccatgagga ttattaaaat gagtattagt
     4321 agctccaatt ttttttgcct cagagttcat aaggtcaata aatttttgta cattgccacc
     4381 accaacatat ctagctaata caaaagcagc atcattagca gaatgaacca aaagagcatc
     4441 tagtaactgc tttacagtaa aagattcccc attttttaga tacatactag agccatctat
     4501 ttgaggtaaa tctttaattt ctattacttg atttaaatct ttaacctcct ttagaactaa
     4561 acaagctgtc catactttag ttgttgaagc tggataaagc ttagaatttc catccttatt
     4621 atatagtatt ttgccagact catagtccat taaaactgca tgtttagcaa ctatagctgg
     4681 ttcattatct gcaaaagaga tattagagct tcctaaaaat gaagtaaata tcaataaaca
     4741 gattaataaa agtgataaat ttcttttcat tgtgagcctc caaattttaa attcatcttt
     4801 tagtatagca tatatttgtc aataattaaa agaaaggagt gattaatata tgaaaaaaaa
     4861 agtaaaattt atttcgtttt ttgttttatt tattttaata atatcatcaa taatcgttta
     4921 taaatcgctt attttcaaag atgatattta tgttgtaagt aaaaaaaatt tacctaatga
     4981 aaaaacacat gttgtagaga atgaaattaa aggtgaagat aataatcaca ataaagtaac
     5041 taaagacata gataaaaatt ttcaaaataa taaaataact atatacataa gtggagcagt
     5101 aaatagacct ggaattgtaa ctatagagtc agacaagaga ctttatgatg ctgtagaatt
     5161 actaggagga acgacaaaag aagcagattt aaatggagtc aatctatcag taagattaga
     5221 agatgaacaa cattacataa tacccaaaat aggtgaagca acatctgtta caagtaatga
     5281 tgattccaat aaaccaaatc agaggaatga gtcaagaggt gagcctaaaa ataaatccaa
     5341 taaaaatata tctaacgaat caaaagtaaa tataaatgta gcgactatag aagagttgga
     5401 ttctctacct ggggttggag aagctacagc caataaaatt ttacaacaca gagaagaaaa
     5461 tggacagttc agttcaattg aggaaataaa aaatgtaaat ggtattggag ataaaaagta
     5521 tgaaaatata aaagatttaa tttgtgtaga ctaaaatata atacaaaact tacatataga
     5581 tatgttatta taaatatgta tgtatatgtg tgtacatgtg gaaatttagt aaaggaggca
     5641 cttttatgaa aaaagaacaa aaaaaactta cagagatgac aacagcaggt ggttgagccg
     5701 ctaaaatagg gccagaggtt ctggcatcag ttttatctca attgcctaaa aatgataata
     5761 tagaaaattt attagtagga ctagatacag cagatgatgc tgcagtttat aaattaaatg
     5821 atgatatggc acttatccaa actttagact tctttacacc aatggtagat gacccatacg
     5881 tatttggtca aattgctgca tcaaattcac tatcagatgt atatgctatg ggaggcaaac
     5941 ctttggttgc tatgaatatt gtttgttttc catcatgcca tgatatggat gtacttgcag
     6001 aaatattaaa aggtgggttt gataaagtga aagaaagtgg agcacttttg gttggagggc
     6061 atactgtaga tgacaaagag cctaaatatg gtctttcagt ttctgggatt gtaagtccaa
     6121 ataaagtttt atcaaatgct actgcaaaac ctggtgataa gttaattata acaaaaccaa
     6181 ttggagtggg tgtattaaat acagccatga aagaaggtat ggtagaacaa catatagcag
     6241 ataaagtaat agaaataatg atacatttaa ataaatatgc agcaatgagt tttgagaaat
     6301 ttgatgtaaa ttcggttaca gatataactg gttttggtct acttggtcat actttagaaa
     6361 tggccaaagc ttctgaagta agtattgaaa ttgaatcaaa acatgtacca attattgaag
     6421 gtgcaataga aatggcacaa atgggaatta tacctgctgg tatgtataaa aatatgcatt
     6481 atgtatctaa agatgtagaa gttgtaggaa atatagaagt tgcagttcaa gatattttat
     6541 acgacccaca aacatcagga gggttgttaa tttcagtaaa agaagaactt gcagaggaac
     6601 ttgtaaaaga tatgaaatta aatggagcaa tagaagctaa aattataggt tctgtagttc
     6661 caaaagggga aaaatatatt acagttttat aaaatattat agagaaagag tgattgtttt
     6721 gagcaaaaga gaattgtttg caatgttgcc ttcagttgat gaagtattaa gtgataatag
     6781 aattgtagaa ataataaatg aatatcctag aagtttagtt ttagaatctg tgagagaagt
     6841 aatagattta aaaagacaat ttatattaag attaaaagaa gatgcatcaa actctgtaac
     6901 aatagagttt ggtgaaataa tagaatctgc aatagagaga gttaagttaa attactcatt
     6961 atctttaaaa aaagttataa atgcaactgg tacagttatt catacaaatt taggaaggtc
     7021 tttacttagt gaagatataa aagatgaatt atggtgtgct gcttcaaggt attcaaactt
     7081 agaatacgat ttggataatg gagaaagagg ttcaagatac tctcatttaa ctagtacaat
     7141 aaaaagactt actggagcag aagatgtact agttgtaaat aataatgcag cggcagtact
     7201 tcttgtactt agcacaatgg ctaaaggagg agaagcaatt gtatctcgtg gagaattagt
     7261 tgaagtaggt ggttcattta ggattccaag tataatggct cttagtggtg cagaattagt
     7321 tgaagttggt tcaactaata aaactcattt gaaagattac aaggaggcca ttacagaaga
     7381 tacaaatgta ttgatgaagg ttcatacaag taattataga ataatgggat tcacagagag
     7441 tgtttctata gaagaattgg ttaatttagg taaaaaatat aaactacctg taatagaaga
     7501 cttaggaagt ggagttttta tagatttatc aaaatatggt ttatcttatg aaccaactgt
     7561 attggattct attcgacaag gtgcagatgt agtaactttt agtggagata agatgcttgg
     7621 aggtccgcaa gcaggtatta tagttgggaa aaaagagtat atagaaaaaa tgaagaaaaa
     7681 tcaacttact agagcactta gagttgataa gcttacaata tgtgctcttg aagcaacatt
     7741 gagaatgtat ttagatgaga caaaagctat agaaaatata cctactttaa aaatgttgac
     7801 atataaaatt gaagaattag aagtaaaagc aaataaacta tttgaaaaaa taactgcttt
     7861 aaacttaaat gcaaacataa atatagaaga tggattttca caggtgggag gagggtctat
     7921 gcctcttgaa actataagta caaaggtaat atcaataact cctgaacata tgaatgtttc
     7981 atctttagaa aaaaaactaa gacttagtga agcacatata atagccagag tgtatgataa
     8041 taaatatgta ctagatgtaa gaactatatt tgatgatgaa tttgatgtaa tagttgagga
     8101 attgagaaaa gcatttaatt aaataaattt gagttaataa atattaaatt tagaagtata
     8161 agtatgaagt tttgattaaa aaatagctac acacacttgt gactttagtc atagcaggtt
     8221 tagagggggc aaaaaatgaa aaatgtagta attggaacag caggtcatat tgaccacgga
     8281 aaaactactc ttataaaagc attgactgga agagagactg atacgttaga tgaagaaaaa
     8341 aagagaggta tttcaataaa cttaggattt acttattttg atttaccaaa caatagaaga
     8401 gctggaattg ttgatgtccc aggacacgaa aaatttataa aaaacatgtt agctggagca
     8461 tcaggaattg atatagtttt acttgttgta gcagcagatg aaggggttat gccacagaca
     8521 gttgagcatt tagatattct aagcttttta aatatcaaaa atggaatagt ggttctaact
     8581 aagtctgata ctgtagatga tgaatttaga gagctagtaa aagaagatat ttgtgaaaaa
     8641 ataaaaggaa cttttcttga agatgctgaa ataattgaag ttgattctat atctaaaaga
     8701 ggtatagaca ctcttattga aaaaatagat aaaatgagtg atgatataga agataaaaat
     8761 gaaaactctc cagcaagact taatatagac agagtatttt caataaaagg gtttggtact
     8821 gtaataacag gcactttaat agaaggaaag ataagtatag aagatgattt ggttatatat
     8881 ccacaggtat taaaaacaaa aataagaagt atacaagttc atggagaaaa taaagataca
     8941 gcttatgcag gtcaaagaac agcaatcaat atatcaaatg taaaagtgga tgatataaaa
     9001 agaggagatg ttttagctgc accaaactca ttggaggaat caatgatgtt agatgtaaaa
     9061 ttatcattgg taaatcattt agataaagga ttaaagcact gggatagatt aagattgtat
     9121 catggaacta gagagatact ttgtagagca gttccactag ataaagaatt aattgaaagt
     9181 ggagaaagtg gctatgttca gttgagactt gaagaaagca tagtatctaa gaaaggtgat
     9241 acgtttgtag tgagacgtta ttctccaatg gaaactatag gtggaggtgt aataattgac
     9301 ccatctccta aaaaacataa gaaatttgat gaaaaagtta tagaagcatt aaaaataaaa
     9361 gaaaaaggcg aactaaaaga tataatagaa gagtacttaa aaaggaattt aaaaaattat
     9421 ccaaatataa aagaaataat gagctatagt ggtgctcatg aagaagatgt aaaaagagcg
     9481 cttgaaacac ttatatcaga agataaagta tttattatag gaaatatgta tatgcatata
     9541 aatcaataca ataaattaaa agaaaataca ataaagcttt taagtgagta ccataagaag
     9601 tataggctta gaaaagggat actaaaagaa gaagtaaggt ctaagataga gagtaatttt
     9661 aagactaggg agatggatat tttattagaa aaattgtcta cagaaaatgc tataaaaata
     9721 gaaaataata tagtatctct attagatttt gaggttattt taaatgataa gcaaaaagaa
     9781 atagcaaaaa aaatagaaaa aagattaaaa tcatgtggag tttcttctat actgacaata
     9841 gatgaagttt ctgaaggaaa tcataactat gctgaagtat tggagtcaat gataggtaat
     9901 aaagtagaga aattagatga tttgtatata atggacaaag atatttatga aaatgcaaag
     9961 aacattttga taaattatat aaaagaaaat aaagaaataa cattaggaga atacagagat
    10021 ttgattgatt ctagcagaaa gaattgcatg attatattgg aaaacttcga tagaaacaaa
    10081 ataactaaaa gagtagaaaa caaaagaatt ttgttttagc agaaaatgtc tgtttttgct
    10141 ttaaggtata aagaaggaat tataatgtcg agtatcgtat aattaattag tataaaatat
    10201 taattaagag gaagtgtaaa gtattgaaga atataatacc tgatgtaaaa ccagatataa
    10261 aagaaagaga agacatgagc cttttattta taataatggg ctcagtactt ttattattta
    10321 ttataaaaga tatagagaaa tatgaaactt tattacatgc catatatacg atatttttag
    10381 tagctacagg agtaattata tttaatacta caaaatttaa agtagattca ttttctacat
    10441 ttttaggatt aatttttata gctactggag tattggagtg tatatattta tttaattcct
    10501 tggggattaa aactaagtct ataatggaga ttaacatcac aatatcagca ataacagatt
    10561 tatttcctat actgggtgta tacttaagtt ttaaatttgt taaggataac aaacaaattt
    10621 attcaagtgt aattttattt atagcaactg ctacattaac aataacatct ttattcataa
    10681 tatcaggaat ttgtgactat ctgggaaatg gtaaatcaca atatgcttta ggagttataa
    10741 tatctatatt cataataatt atttctatca ttagtggaat tgaattaaat tcaagttcaa
    10801 ataaaggtaa atgggaatat gatgaaaaaa agatttttaa cagaataata gtaataataa
    10861 tcttatcaag agttccaaat ctattgcata tagttattga taatagaaat gtagagagga
    10921 tattatcaca aataataata aatgtagcct tatattattt atataattac atagtatcta
    10981 agaatattaa gaaaacagca ctggaattgc atgatacgaa tgaagaatta acaaaaaaaa
    11041 cggaaagttt aaaagaaaag aataaaaaat taatctatga gacagaaaag atagaggaac
    11101 ttaagggaat acttatgaaa agagaatcta ggctacaatc tacattagat gtagcagtaa
    11161 atagcatagt agtatttagc aaagatggag aaattacata tgcaaataag agctttagaa
    11221 atacttttgg agactataaa gaacaagata aattgactga taaaataaaa aacttcaatg
    11281 atttgataga aagcattcat tatgtgtttg ttcatgagaa gaatgcagag aaacttatta
    11341 gtacaagcga taataaagtt tatcaagcta tatttgcacc tttaattatt gcttcacaaa
    11401 cagaaggagt attgtgtgta ctagaagata agactaagaa aaaggagtac gaacgcaaac
    11461 ttattgaagc aaataagaga agtaaagatt ttttagaaag tattggagat ggcatagtag
    11521 tgcttgaagg taataacaaa atatatgtta ataatgcatg tagggaaatt tttaaaaata
    11581 atttagagag tattgatttt tcattagtat gtaaaagtga agaaagtata gaaaaaagat
    11641 atgtaataga tggtgaagta aagtatgtag agatgagttt ttctcagtat actaatgctg
    11701 gtactaataa aactattatt gtaatacgtg atacaacctc tcgaaaaata tctcaaataa
    11761 aacttgagga aagtcaatca tcatattcaa gatttataga tatattacct gatggcatat
    11821 gcttattaaa agaagattta agtataaact atgcaaataa gtcgttttta gatatgctag
    11881 cttttacaaa catagatgat ataaaagatt ctaatattaa actattaatg aatgcgaata
    11941 ctgaagaaaa aatgaaattt acggataata tggctaaagt tttaaatgaa aatagaagta
    12001 tacttttact ggaatatgaa ttgataaata gttatgatga tatagtagaa gtagaattaa
    12061 gtgcattacc ttttgctata tacaatacta gatacataat gcttattata aaagatttag
    12121 tacataagaa atattccgag caagcagaaa aagaactgct tgaaagattt aaaacagata
    12181 aaatcaaaac ggagtttttt gcaaatatgt ctcatgaact aaaaactcca ctaaatgtta
    12241 tatctagttc aaatcaactg gtagattctt tctatagaaa tgaaaagata aaagattaca
    12301 ataacaatat taaatctcat gtggatttag ttagacaaag ttcttatagg cttcaaagat
    12361 taataaataa cattatagat ttaacaaaga tggagtctgg gttctataca ctaaaacttg
    12421 cgaaatacaa tattgtatct gtaatagaag acttatttat gaatatagaa gagtatgcac
    12481 ttagaaaaga cataaaaatt ctttttgata cagaccttga agaaattaat gtttatatag
    12541 acaaggttga aattgagaga attatgttga atctattgtc aaactgtatt aagtttactg
    12601 ataatggagg ctggatatat gtaagtattc attataaaat agataaagta atcataagtg
    12661 taaaagacac tggagttgga ataccacaag ataaattaga gttgattttt gaagaatttt
    12721 cacaagttga taagacttta tccagaaata ctgaaggaag tggaataggg cttgctatag
    12781 ttaagaatct agtaagtctt catggtggag atatagaagt agtaagtgaa gtgaataaag
    12841 gcacagagtt cttaatttca cttccaatga agggatttag tagtgaacat tacacagaag
    12901 ataagagaat atataatatt caagaaaaaa taaaaataga attttctgat atatattatt
    12961 aaaaatgatt atgagcaaaa ataaaataaa aatactattt gtttaaatat ttaagttgta
    13021 agaaataggc tatcataagg taaattatta tgatagccta tttttagcta ggttttatat
    13081 ctatatgtga gaacatataa tctctaaatt accttcaaaa ctaaaatctc caaagtcgtt
    13141 tggtagtata aaatgcatac ccttttttaa attgtattct acagaattgt gtataagttt
    13201 tccatttcca tccaacacac tgtataaaga aaaaggtgta ttgtgtgtaa aattacattt
    13261 tccaaaaaca tctaatttat aaacactaaa aaattcgttt gacacaaatg ttgtgcattt
    13321 aaagtctgat gttgatacga ttttatagtc agtatcgaaa tttatatgag gaacattcgt
    13381 aacatctatt gatttttgaa catgaagttc acgcttatta cctgaattgt cagttctatc
    13441 ataatcatag actctataag tagtatctga gttttgttga gtttctaaaa tcaatgttcc
    13501 tttgcatata gcatgtatag tgccacttgg aacatagaaa aagtctccct tttttatatt
    13561 aacctttcta agtagtttgt cccattcatt attatttacc atatttacaa attctttatg
    13621 agattttgca ttatgaccta ttataatttc tgcatcatca gaacaatcta ttacatacca
    13681 acactctgtc ttacctaact ctccattttc atttttttta gcatattcat catttggatg
    13741 aacttgaact gataaattat catttgcatc caatattttt gtaagtaaag gaaacttatc
    13801 accaggtgtg tttccaaata attctctatt tttattccac aattcagata gttttttacc
    13861 tttatatttt ccattttcta ttaagcaatc tccattttta tgagagctta tagcccaaca
    13921 ttcaccagtt gttggcgaat ctatttcata attaaattta tctttaagtg cagtaccacc
    13981 ccatattcta tccataaaaa taggttttaa aaataatggt tccataatcc tgttcctcct
    14041 atgatattaa atttatccta caaataatta tatataaata gattataaaa tcaatagtaa
    14101 aaaagttttg tcccttgaaa tgacataaag tataaggagt tattaaataa gataaactat
    14161 gatatataat ataaagttct aaattggagt gataattatg gagttgttta aggcagaaag
    14221 caaaaaatat aaaaaagaat ttttaaaatt attggagcaa tgggttagta taccttcatt
    14281 ttatgataga aaaactgtat ctaaagacat gccttttgga aaaggagtat atgatgcatt
    14341 aaactggttt gaaaatctag gtagagaaaa caatttcaaa gtaaaaaata tagataatca
    14401 tgctgttcaa attgaatatg gaaatggtaa agagtatgta gatatatttg gacattgtga
    14461 tgttgtaaat ccaggtgaag gatgggatag tgaaccattt aaactaaata ttataggtga
    14521 taaattagtt gctagaggtg tatctgataa taaagggcct atgatagtga attttttagc
    14581 actaaaaatg ataaaggatt tagatattaa tttaaaaaga aaggttagat taatagctgg
    14641 gggaaatgaa gagagtggat ttaaatgtat aaagcattat tatagcaaag agccatatgg
    14701 tgtatgtgga tttactccag atgctaaatt tcctgtatta aatggagaaa aaggtggagc
    14761 aataataaag ttaatttcaa atatagacga taaatcttta tatataagtg gtggaataga
    14821 gtttaataca ataccagata aagtttatat aaagaatgta gaaaaattgg gtaaagataa
    14881 tatatgtttt gatattaaca atatatcgat aaattatgat aatggcaatt atattgtgta
    14941 tggaaaaggt ggtcattcat ctaaacccga aaaatcaata aatcctatat tagctacaat
    15001 taaacttttg tcagaaaata ttgatgaaaa atggacaaaa gatttatata agttgataaa
    15061 tcaagataat ataaatggaa acttatttgg tttaaatatt gaggggaaat gtggaatttt
    15121 atcaatggtg ccaacaataa taaatatagt agatggaaaa ttagaagtag ttttaagcgt
    15181 tagatatcca gaaatattaa ccattgaaga tataataaaa aaatttaatt tgtatatgga
    15241 gcaaaataat attaataaat ttgagcttat tggggaaaat ttaaagcaag caaattatat
    15301 agatagaaat tcaaaacttg taagaagttt gcatgatata tatataaagt attctggaga
    15361 tttaaaaaat gatgttagag taactagtgc aggaagttat gcttctgaaa tgaataattc
    15421 agtaatattt ggttgtgaat ttcctgatgg tagctttggc aatgtccata gtgcgaatga
    15481 atttgctagc ttagatagat ttataactgc aataggtata tatgcagagg caataattac
    15541 attatgtaat aaaatataaa tacaaaatgt cccttatata gacatattat atatacaatt
    15601 aaaattttac ttgatatata attgaaatca aagagaaggt gatacaatga taactaagaa
    15661 acatgcaata atagtaaaga gtttaagcaa taaagatgga tatatgacat caaacgaatt
    15721 agctatcaaa ttggatgtgt ctactaaaac tattaaaaga tatatagcag atttaaatag
    15781 tgttttaagc aaatatgatt tggtaatagc ttcttctaga ggtataggat acaagttaag
    15841 cggttctaaa aacaatatag ccagagctgt taaagaagca aataaatata ttgatggttt
    15901 tttagatgac tctgaagagt caagaatgtc aaatataata tgtatgctta ttaatagaaa
    15961 ttatatgagt atagaagcaa tggcagaaga attaaatcta agtatagccg ctataaataa
    16021 attgtctagt aagcttaaaa gaaaacttga aaagtatgac ttagttataa aatctaaacc
    16081 ttactatgga agctatatac atggtgaaga aattaatata aggcaattaa ttactgacta
    16141 tgcaataaaa ttagatgaaa aaagtaaagt gaaggtgttt ttagatgaca ttagtgaaaa
    16201 cgatatacac tgtatagaaa gtattttaga aaagcactta agagaaaaag atacaataat
    16261 ttcagataaa gattttaatc ttttgctttc aaaaataatc gtttctgtat ttaggagtaa
    16321 gagaggtcat agtaacaata taaacttaat ggatacaagt tatagatttc ataactacta
    16381 ctttatagaa aatctaatga aagaaatatc tgataaaatt ggatttaaac ttattgaaga
    16441 tgaagtaatt tatatttcaa attattctgg tgtaatagcc tataaaggta cacaaggaag
    16501 aaaaaacaca agtgaaattg aggaacgaat aagtactgtt ataagtagtg cacttcaaga
    16561 tatattcctt atttcaggta gtgattatac aaaagatgat gagtttatgg ttgcaatctc
    16621 tgaccatata aagagatttt taaatagagc tagagctaat gtaaaatcaa ataatccttt
    16681 acttcatcaa ataaaagaga agtttcctat agcttttaat ttagccgtgt ttttatcaaa
    16741 taaactagaa actgagttta atttaaaact tgatgaagat gagcttggat atatagctat
    16801 ccactttgct gcttcaaatg aaagaatgaa aaaaaatact agtaagaaaa tttgtatagt
    16861 ttgtcattat gggataggaa caggacaact tatttctgaa aagctaaaac agaatataag
    16921 tgatttaagt gttgtgggtg tttatccagt tagatatctc gatatggcta taagtcaaga
    16981 tgttgatttg atagtatcta cagttgaatt aaaaggatat gaaaaacctg tcttatatat
    17041 agaaaatata tttgatgata gcttaattga aaacgtaaac aaagcctttt atgaaaagga
    17101 agaaagaaga aagataataa gtaacatgtt tgatgaaaaa gcttttttca gtataaaggc
    17161 tagtactaaa gaagaggtca taatttcact ttcaaataaa ctaaaagaaa gagattttat
    17221 agaggaaagt tctataaaaa gtataataga tagagaaaat atttcatcaa ctgaaatagg
    17281 aaatctagta gcaatacccc acacaatagt gaagggagat aaaaaatcta taataggagt
    17341 tggaatactt gaaaatccaa taatttggga taagcaagag gttcagctag tatttatggt
    17401 attttttaat actaaagaaa aacataattt ctcgatattt aaataccttt ataattttat
    17461 aaaagatgaa ggaggagtaa gaggaattat taaaatatgt gactttaata aattaatggc
    17521 acttataggt aattaaggag gaaattaata atgggaacaa acatatttaa caaagaatat
    17581 gtatttttaa atgttgatgc taagtcaaag gttgaagttt taaaatttat atcaaaaaaa
    17641 gctaaagact taaacttggc agaagatgag agcttagtat atgaaggttt aatggctaga
    17701 gaagaacaat ttactacaaa tttaggagaa tctatagcaa tacctcacac taaaaatgat
    17761 gcaattgaaa atccagcagt agtagtattg aagtttaatg aagatgttgt ttggaatgaa
    17821 ggtgaggata aggttaaatt agcaataagc ttacttatgc caggaaaaag taaagagaat
    17881 attcatctta aattattatc atctctttca agaaaattaa taaataaaga atttaaagat
    17941 tctcttttaa aaagtgataa tgttgaagaa atatctaact taataaatga agctttaggt
    18001 ttataattaa acactaaggt tataaaataa aaaaataatt aactaaaaat aggaggaatt
    18061 tgaaatgaat aaaaaattag tagcattatg tgcttgtcca atgggactag ctcacacttt
    18121 catggcagca gaagcaatag agcaagcagc aaaggcatta ggttatgaag caaaagttga
    18181 aactcaaggt gctgatggtg tacaaaatga gttaacaaga gatgatatat taggagcaac
    18241 tatgataata catgcagtag caataacacc agaaggtatg gaaagatttg atggttgtga
    18301 agtttatgaa gttgaattac aagaagctat aaaaaatgca gaaggtgtta taaaagaaat
    18361 agaagaagat ttaggaatat aataaattta ttgtggagga aatagaatgg caattaagaa
    18421 aaaagttata aacagttcag gtgcttctac aacaggagga aataatgtag gaaaatctac
    18481 tccatctagt ccaactcaat caaagggaaa tggaaaatgg aaagaaataa gtaaacatat
    18541 aatgacaggt atatcatata tgataccagt tttagtaatg ggtggactta taggggcctt
    18601 atctcaatta ataccatatg ctatattagg acttgaccca tctgtaggta tagttgatgc
    18661 tatgaactca ggagaattta cagggttcaa gcttagtctt ttaaatatag cacaattaat
    18721 gtcaaatttc ggatttactt tgtttggatt tgcgatacca ctgtttgcag cattttgtgc
    18781 aaactctata ggtggaaaaa cagctttaat tgcaggattt ataggtggat atatagctaa
    18841 taaacctgta ggtgtaccac aattcgttga tggacaatgg actgaagttg ttccagttgc
    18901 atcaggattt ttaggtgcaa tactaatagc atttataata ggatattttg taaaatggtt
    18961 aaataaatct ataaaagttt ctcataactg gttagctttt aaaacaacat ttttaatacc
    19021 attaatagcg tcattagctt gtatggtatt gatgatattt ataataactc catttggtgg
    19081 attaattaat gaaagtatga aaaacttctt aacagcagca ggagcagctg gagaatatgt
    19141 atatgcaaca gctttggcag cagctacagc attcgactta ggaggaccaa taaataaagc
    19201 agcaggattc gttgcacttg gtttaactac agagaatgta cttccaataa cagcaagaac
    19261 aatagctata gttgttcctc caattggatt aggattaaca acattattag ataaaagatt
    19321 agtaggaaga agagtatatg atagacaatt ctatcaagca ggaaaaactt ctatattctt
    19381 atcatttatg ggtatatctg aaggagcaat accatttgca cttgagagac caggttttgt
    19441 tataccttta aacatagttg gaagtgtaat aggagctatt acaggtataa ttctaggagc
    19501 aattcaatgg ttcccagagt cagctatatg ggcatggcct ctagtagata acttatttgg
    19561 atatataata ggtatagcag ttggtgctat attcatagca gtaggaaata tattctatag
    19621 aaataaatta ataaaagatg gtaaacttgt tgtagattat atcgactagt atttaatttt
    19681 tactatctat aaataacttt ggctggtaat atggttttaa tcaatattac cagccttacc
    19741 tatatgatta attaaaggcg gtggagataa gatatggata agatagaaaa agtaagagag
    19801 tattttagag aatatgatat agatggattt cttataaata gtcctacaaa caagttttat
    19861 gttggaaatt tatttagctc ttcaggttat gtttttataa ctaaagaatc acaatatata
    19921 atagtagatt ttagatattt tgaagaaatt aaaagaaaat ctagtttgtt taatgtagta
    19981 ttaatggaca aaactagaac tcattttgat ataattaatg atatttgtag agagcagaat
    20041 ataaaggaaa ttggatttga gggaaatgaa gtatcttttg atttatatag aagtatgagt
    20101 aataagttaa gtgcaacctt aaaatcagtt gatttatcta cattaaggga aacaaaaaat
    20161 gaagacgaaa taaaatatat aaaaaaagct tgtgaaatag ttgatgctac attttatcat
    20221 atagtagact ttataaaggt tggtatgact gaaaagcaag ttgaaaatga aattgttaga
    20281 attataaaag atttaggtgg tcaaaaagaa tctttcgata caatagttgc atctgggctt
    20341 agaggagcat tacctcatgg aaaagcaagt gaaaaagtta tagagtatgg agattttgtt
    20401 acctttgact ttggtgctaa gtataataat tattgttcag atattaccag aaccatttgt
    20461 atgggtacta taaataaaga gttagaagaa atatataata tagtaagaaa agctaatgaa
    20521 gagtgtataa gggttttaag accgggaatg actactggag agatagataa agttgcaaga
    20581 gacataattg gttcatatgg atatgccaat aattttggtc ataatctggg tcatggtgta
    20641 ggaattatgg ttcatgaata tcctgcatta gctcctgaat caaatgaagt attaaaagaa
    20701 ggaatgattg taactataga gcctggtata tatgttccaa gtcttggagg cgtaagaata
    20761 gaagatgatg tacttataac acaagatgga tgtatgagat tgactacatc tactaaggat
    20821 ttaatagttg taagctaaaa ataaattaga taaatatttt ataacaataa gaaaagaaga
    20881 ggtaaattta aatgtataat tttgatgata aaccagatag agtaagtgaa aagtgtagaa
    20941 aatgggattt aaatattata agagataaat ttggagatat tagagaagat tttataccaa
    21001 tgtggattgc tgatatggat tttaaaatac caacagaaat agaaaacaaa tttattgaag
    21061 ctgtaagaag aggtgtattt ggatatacat actgttatga tgaattttat gattcagtta
    21121 ttggttggca aaaaaatatg catgaggtaa atgtagaaaa agaatggatt acattaactt
    21181 atggaactgt atcaacactt cattatgtag tacaagcttt ttgtaagaaa ggtgatagta
    21241 taatattaaa tacaccagtt tatgacccct ttgaatcatc agcaaaaaaa caaggtgtaa
    21301 atgtaatttg taatacatta gatgttgtaa atgatagata ttatataaat tttgataaat
    21361 tagaagttca aattaaagaa aataagccta aattaatgat gttttgtaca ccacataatc
    21421 catctggaag gatatggaca attgaagaaa tgacaagggt agcaactata tgtaaagaaa
    21481 ataatgttat tttggttgca gatgaagtac atgcagagca tattcattat ggtacattta
    21541 attcaatact taagattgga aaagagcttt tagaaaatgt tattctattg acatcaccaa
    21601 ataaaggatt taatcttggt ggattaaaga cttcttattc aatagttata aataaggata
    21661 taagggacaa atttagaaat aaattaaagc aaaattctat aacttctcca aatgtgtttg
    21721 gaataatagg attgataacc gcttataatg aatgtcatga gtggttaagt ggtgtaaatg
    21781 aatatataaa atcaaattat gaacttttag aaacttgggt aaataaatat aataagataa
    21841 aattgatgaa aatggaatca tcttacttag cttggatgga tataagtgga ttaggtatta
    21901 gtgcaagtga gtttacagat aaattggcta tagatacagg agtattatta gaagatggaa
    21961 gtcattttgt aaaagatggt gagaagtatg ttagaataaa tttaggaact caaaaggaaa
    22021 atgtaattga agccttaaat agaatggatt tatttttaaa gtctttataa aaatttggtt
    22081 gaattagtat tttatataaa tttaaactat taatatggta tatacataag tagaatttgt
    22141 tttagattac ttaagtatat accatatttt atataaaaat taaatttgca ttagtcatgt
    22201 gataactatt taaattcata tgtattttgt atgtataata gatataatta ttatttaaac
    22261 ttattgttta tttaattttt caatagaaaa agatttatct gtaagtttaa aattatcttt
    22321 tataccctta gttaagtata ctttcttatc cttagttatg aatatagcat ctacattatc
    22381 aattttatta attaaattca tacctttttc aagaccaaga ccaaatgtag aagtggataa
    22441 ggcatcacaa ttaatagatt tatcagagat tattgtaaca ctacttaaat tgttttcaaa
    22501 aggataccct gtaaatggat ttaacatatg atgatatatt tttccgtctt tttcgataaa
    22561 tctttcataa attccagaag taacaacaga cttatttgta gtttctatgt ttcctatgga
    22621 ttcaccattt ttactagttg ggtcttgaat acctatttta aaaggtttat catttttacc
    22681 attttctaaa gtaaatatat ttcctcctaa gtttactaaa ccttttttta catcctctga
    22741 ttttaaatag gctacaattt tatcagcagc atatccttta gctatggcac ctaaatcaat
    22801 tttcatattt tctttggtta attttataga tttatttttt tcatccaata tgatgttctt
    22861 ataatcaata agaggcagaa gtttttgtat cttagaatca cttggaactt tagctttatc
    22921 tgttccaata gcccataaat ttgataatgg accaattgtt atgtcaaaat atccatctga
    22981 taatttggaa tattgtatag cttcttttac gacgaaaaat gtatcatcag aaactctgac
    23041 aaatttttta ccagcattat catttatttt agaaacatca cttcctggta tatgagtgct
    23101 cattttgttg tcaatatatc ttaaaattga atcacattca tttaagattt tatcactttt
    23161 ggattctttt acattaaata cagtaacttc gtttactgta ccaaggtagt agctagtttt
    23221 actatattta gtattctggc ttgaactcct aaaaaatgaa aataaaatta atgctaaaat
    23281 caatatcaca aaaataaatg tgattttttt cttattcatg gtatattcct cctatgaaat
    23341 tgtgttatta ttttattata acataatttc ataggaggac aaaaaatgaa gaaaaaagac
    23401 attcttctta ttttaggcat tttggttgtt attacagcat gttatgggat aataaatgtt
    23461 ataaattcta aaaatgctgg aaatattgag atttatgtag ataataaatt gtataaaact
    23521 gtttcaataa atgccaaaga agaatttaaa atagagagta gaggggggta taacatagta
    23581 aagattcatg ataaaggggt tgagatagta gatgcttcat gccctgataa ggtttgcgta
    23641 catactggat ttataaacaa gccaagtcaa agcatagttt gtatacctaa tagagtgagc
    23701 ataaaaataa atacaaatga aaagaatgat aatcaagaag atataatttc taaataaaat
    23761 aataaaaata attgaaagga attggtgata attatggtta acttaggaaa cgattgggat
    23821 gaattattga aagaagaatt tgaaaaagat tactacttaa acttaagaaa atttcttata
    23881 gaagaatata aaacacgcca aattttccca aatatgcaca atatatatga agctttaaaa
    23941 cacacttcat acaaagacac aaaagtctta atattaggac aagaccctta tcatggagat
    24001 aatcaagctc atggtttagc tttttcagtt caaccacaag tgaaaactcc accatcttta
    24061 ttaaatatgt ataaagaatt aaaagatgat ttaggttgtt ttatacctaa taatggatat
    24121 ttaatgcctt gggcagacca aggagtgtta cttttaaata ctgctcttac tgttagagcg
    24181 catgaagcta attctcataa gaacaaagga tgggaaattt tcactgatag agtaatatct
    24241 attttaagtg agcgtgaaga cccagttatt tttgtactat ggggaagtaa tgcacgtaaa
    24301 aaagtagagt tgattgatac aagtaaacat tatattttgg aagcacctca tccaagccca
    24361 ttgtcagcaa gtaaaggctt ttttggatgt aaacacttct ctaagataaa tgaaattttg
    24421 aaaaagctag gaaaagaacc tattaattgg cagatagaaa atatataaaa gtaaaaggta
    24481 acaaaatgat aacaaaacaa caataaacaa tgacattgtt gtaactaata gaaatatgct
    24541 taaatagttg aaaatactat gtatgcatat ttctattttt ttgtcaagtt gacaactctt
    24601 gaaaaaatag ttacaattta gttacacaaa aaacacaaat aaaaaaagaa acttacaaag
    24661 cagaatttta tgaagcaaaa tatatagtgt aaaaaatctt agggaggaaa ttttatatga
    24721 attttaaaaa attgagtaat gctaaagtct cggtaatggc agttgtttta tcagttggaa
    24781 tattatcggg atactctgcg cttaataaac aagtaacatt agtagtaagg ggagaagaaa
    24841 gaactatatc aacatttagt tctaatgtag gagacctttt agaatctcaa aacattagct
    24901 atgattcaaa tgatatagta actttagatg aaaatacaaa gttatctaat ggagataaaa
    24961 tagaagtaat tgatgtaaag gaaaaaacag ttgtagaaac taaagacatt ccatttgaag
    25021 ttacagttgt tgaagatggc tctttattaa agggtgattc taaggttaaa gaagaaggaa
    25081 agtctggtaa aagtgaatta atatataaag aaacttatca caatggaaag aaagtagaaa
    25141 aggaatttgt gaaaaaggtt gttaaagctg agccagtaaa taaggttgta agcaaaggga
    25201 caaaggtaga aatgcaagta gcctcatcaa ggggagaaag ttcaagaaga atggcttcat
    25261 catcatctag tagcagtagt agtaacaatg gtaaaaatat gagagtggta gcaactgcat
    25321 atactggtca cagtataact gcaacaggaa caaaacctaa gtggggaaca atagctgtag
    25381 accctaaggt tataccatat ggaactaaag tttatatacc tcaatttgac atggtgttta
    25441 ctgcagaaga ctgtggtgga gcaataaaag gaaataaaat tgatatctac atgaatgatt
    25501 caaaaacagt ttataactgg ggaagaaaaa ctatagatat acgtatatta aaatagttaa
    25561 ataatttata tttttactgg aaattatatt ttgattatat agagaagaaa ctctttatag
    25621 attctaaaaa tataatttcc agttttttat tttggatatg taataaaaca ggtaaatttt
    25681 aaccatgtat agataaaagc tatttaaatt ataatttact atcatataaa tctataaaaa
    25741 ttgttgcaaa gagtcaaaat atatgatatt atagatttcg ttgagaagga tttcattatg
    25801 ggagtggatg ggtgccggtg tgccctctag tcttcaaaac tagtatgagg ggttaagagc
    25861 ctcttaggtg ggttcgattc ccacgcattc ccgccaatat gtaaaaggta tcttttaatt
    25921 aaaagatacc tttttaaatt tattttaaga taatttattt ttaataatat ataatgtttt
    25981 gtattatata atgtaaattt tctaatacaa aacattatat atttagataa aaatctagga
    26041 gtgtggaaag atggaaaata attttacaac gagaactagt tttctagttg gagatgatgg
    26101 gataaaaaaa cttaataatt caaatataat agtatttgga gttggaggag ttggtagttt
    26161 tactgttgaa gctttagcaa gagctggagt aggcaatatt actattgtag attttgatga
    26221 tgtagatata accaatataa atagacaaat accagcccta cattcaactg taggaagata
    26281 caaagttgat gtcatggaag aaagaatatt agatataaat cctaatatca atataaaaaa
    26341 aataagaagt ctttataata aagatactag tgacgaaata ctaactgaaa gatatgatta
    26401 tgttgtggat gcgatagaca tggtaagttc caaaatacat ctaattgaaa cttgtgaaaa
    26461 aaaaggattg aaaataataa gttctatggg tatgggaaat aaattagacc caactaaaat
    26521 agtcgttaca gatatacaca aaacaagtac ttgtcctcta gctaaagtta tgaggaaaga
    26581 acttagggat agaggtatca aaaagttaaa agtagtatat tctactgaac aaccaataga
    26641 acttaagaag aaagtaatga atggtagaaa agtaacacct ggtagtgtat catttgtccc
    26701 atctgtagga ggtttaataa tcgcatctgt aatagtaaat gaactattag gacaataagt
    26761 tttaaatatt ttaatttata aattttatat acaaatttga aggctagaag catgaataca
    26821 aagcaatttt atattcatac ttttagccta attattttat attaccttat tttatgtagt
    26881 tgtagtttta ttttttagat tatgtatcta tttcataatt atattttgta aatataattt
    26941 ctccatcact ataaaaaaca atatctccta ttctatctgt tctataaatt gatacatggt
    27001 tttgttttag attattaata acctccctat gtggatgacc atattgattt ttatatccac
    27061 atgatattgc agctatatct ggactaattt tttttataaa ttctaaagat gaagaagaat
    27121 tacttccatg atgacctatt tttataaaat taatattatt taatttaaaa gaatgtaaaa
    27181 tttctttttc attttcttct tcagcatcac ccataaataa aaaagaatta tcattaaaag
    27241 ttaatttgaa aactatagaa tttaaattag attcttcttg tatatatgat ggacttaata
    27301 catatatatt tatattgtta tctatgttta aaacatcatt tttatagaga tgctgaatag
    27361 ataagttttt atccgtacaa gaatttatta gattttgata agccttacta tcagttgatt
    27421 gttcaggcat atatatggag tttacattaa atttttttat aatattatca agacttccta
    27481 tgtggtctga atctggatga gttgctatta taatatcaat agtttttatt cttttttgtc
    27541 taagataact aataattata ttttcgctat cttcatcacc tccatcaatt aatatattct
    27601 tatttgtggg tgtttgaact aaaatactat caccttgtcc aacatcaatc atatgtatag
    27661 atagtagaga ttttttgtca cagccgatta aaagtgatat tataataagt attgataaaa
    27721 aaattttatt tctcaataaa aaatcactcc attcattaca tgtttatata aatttatgat
    27781 gggtaatata agatatatat gacttattat attttatatt ttaaacaata taaatgaaat
    27841 taataccata ataaaattgg agttttttat atatacaaaa taaaattttt aaaattaagc
    27901 aataaaaaag gtaggaggat tttatgtttt tcaaagatat tgaactaaat tcaaaaaaag
    27961 agttagaccc atattttgat ttagtagatt atgaagcttg tgagtactgt tttagtactt
    28021 tatatatgtg gcaacatgtt tataagacag gatattatat aggtgaagat tttgccgttt
    28081 tagttgggga atacgaagga gatagcttct caatattacc acttgctaaa aaagataaac
    28141 ttccagaagt tgtagatttt gtccttgaat atttttctaa aaataataaa aaaatatact
    28201 taagaggtat tactactgaa gtagtagagt ttttaaaaga aaaatatcca ggtagatttg
    28261 agtatataga agaaagagat ttatttgact atatttatga tgcagaaagt ttaagaactc
    28321 tagctggtaa aaagaatcaa aagaagagaa accatataaa ctatttctta aaagagtatg
    28381 caggtagata tgaagctaaa ttattagata aagaaaactt tgatgagtgt ttagtactaa
    28441 tgaaggaatg ggaaagtaat aaggaagaaa ataatgaatt tgatgaaagt atggatgatg
    28501 aattaatagg tatcaaaaag atatttaacc attatgacat attaaaagat aaggtaaaag
    28561 tgtttggtgt ttatgtagat ggaaaattag aagcttttag tataggagag cttttaaatc
    28621 caaatatggc attgattcat atagaaaaag ctaatcccga tataagaggt ctgtatccat
    28681 ttataaatca acaatttttg gtaagtgaat ttaaggacgt tgagtttgta aatagagaag
    28741 aagatttagg tattgaagga ttaagaaaag ctaaactttc ttatcatcct tgcagatttg
    28801 ttgaaaagta cagtgttagg gaggcttaat agactatatg gagattaggt atgctaaaga
    28861 agaagagata gaaagtataa aagatatttg gagttattgt tttaacgata cggagagttt
    28921 tatgaagtac tattttaatg ataaatataa atctgaaaat actgtagtag ctttagatga
    28981 aggaaaaatt atttcttctc ttcagctaaa tcagtataag ttattactaa atagcaaggt
    29041 atataataca tcatatgtgg taggagtttc tacacttcca gagggaagag gagctggata
    29101 tatgaacaaa gtaatgaaat ttactttaaa cgaattgtat aaaaaagggc aattagtttc
    29161 tatattgatg cctattgact atagacttta tagaagattt ggatatgagc attgttacga
    29221 ccagattgaa tatactatta acacagatga tttaaaaaat tttaagtcaa gtggaaaaat
    29281 gataaagtct aatttatcac aaattgatga tttgatacaa atagatagaa cttttttaaa
    29341 tgaggttaat ggtaatgtgt taaaagatga acattattat gaaaacttat ttaaagagat
    29401 tcaaagtgaa gatggatttt tatacattca tgaaggaaat gaaaaagatg gatatatagt
    29461 ttattttctt caggaagata aaatgtttgt cagagagtta ttttataaaa atatagatgc
    29521 ccttaaatca atgcttaaat ttatatataa tcacaatact caatgtaaga tagttactat
    29581 atctactcct acaatagata agataaggtt tatattagac aaccctaaag atagtgatat
    29641 aaaaataaaa ccttttatga tgggaagagt tataaatgta aagaagttta ttgaagatat
    29701 agatattgag aaggatatta atagctcatt taatttatta atagaagaca agtttataga
    29761 tgaaaataat ggtctattta aaatttctat acagaataag aaggtatctg tagagcagtt
    29821 agataaaaaa ggtgcagaaa agcctcaaga agattttgat attaaattag atataaatac
    29881 cttaacacag ttatcatttt cttatataga tgtaaatgaa gctattttct taaatgatat
    29941 aaaagatgtt tcagaagaaa cactagaaac acttaattgt attttttcta aaaaaaataa
    30001 ctatataaat gaatatatat aggtagagta cgttaaaaga ttctaaaaat cttttgacgt
    30061 atttttttgt ttagataaca atttatcttg tttaaaaatt ttctatttga aaataatcta
    30121 aataatatac ttttatatgg gagtgattat aattcaaata agaagaccat tattaattat
    30181 attaatgttt gtggtagcta tatcatttat cattacaaat aacacaaaaa acgacgaaga
    30241 acttaatgat aagataataa ctatacaagg agttgtcaaa ggaaaaattg aaaagaaaag
    30301 atacaatcag tacaaggtag gggtttttct aataaatgat tatacaaaaa ataaaaattt
    30361 gaagattggt cagaaagtta atattacagg taaattcaaa agtttagata aaatgaagta
    30421 tgatgatttt gattatggaa ggtatataaa aagtactgga tataaaggta tagtgtatat
    30481 aaataactat aaaattattg ataaaaataa actttactct ttaatagggg aaataaagtt
    30541 ttatattagt aaaacttata ggtacttata taagaaaaac tcagatttta taaattctat
    30601 actattagca gaagttgaaa atttgactga ggaacaaaaa gaaattttta ccagaactgg
    30661 aactagtcat gtaatatcta tatcaggtct tcatacagga atattatgtg taattatatc
    30721 ctttctatta agaggtataa ataaattgta taaattatta atactgtcga tttttataac
    30781 tttatattgt ataatggtag gtgcgtctcc atccataatt aggtctatag catttgttat
    30841 gattttttat ttatcaatat ttattgatag aaagaaagat ggaatatcgg cattgtctct
    30901 aattgggata attttaatta tg
//
LOCUS       DAEMGG010000039        30874 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEMGG010000039 DAEMGG010000000
VERSION     DAEMGG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30874
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.039"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..374)
                     /locus_tag="KPK24_001646"
     CDS             complement(<1..374)
                     /locus_tag="KPK24_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG2299323.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIK"
     gene            complement(427..786)
                     /locus_tag="KPK24_001647"
     CDS             complement(427..786)
                     /locus_tag="KPK24_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG2299324.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            complement(840..1925)
                     /locus_tag="KPK24_001648"
     CDS             complement(840..1925)
                     /locus_tag="KPK24_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG2299325.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKAKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            complement(2096..3475)
                     /locus_tag="KPK24_001649"
     CDS             complement(2096..3475)
                     /locus_tag="KPK24_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG2299326.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(3468..4148)
                     /locus_tag="KPK24_001650"
     CDS             complement(3468..4148)
                     /locus_tag="KPK24_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG2299327.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(4540..5619)
                     /locus_tag="KPK24_001651"
     CDS             complement(4540..5619)
                     /locus_tag="KPK24_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2299328.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      5907..6022
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6197..7567
                     /locus_tag="KPK24_001652"
     CDS             6197..7567
                     /locus_tag="KPK24_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2299329.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFVGLILTVFFMRK
                     TDKSIS"
     gene            complement(7833..8444)
                     /locus_tag="KPK24_001653"
     CDS             complement(7833..8444)
                     /locus_tag="KPK24_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG2299330.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(8434..10797)
                     /gene="lon"
                     /locus_tag="KPK24_001654"
     CDS             complement(8434..10797)
                     /gene="lon"
                     /locus_tag="KPK24_001654"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG2299331.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            10977..11489
                     /locus_tag="KPK24_001655"
     CDS             10977..11489
                     /locus_tag="KPK24_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2299332.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            11477..12004
                     /locus_tag="KPK24_001656"
     CDS             11477..12004
                     /locus_tag="KPK24_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2299333.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(12075..13325)
                     /gene="clpX"
                     /locus_tag="KPK24_001657"
     CDS             complement(12075..13325)
                     /gene="clpX"
                     /locus_tag="KPK24_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG2299334.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(13344..13928)
                     /gene="clpP"
                     /locus_tag="KPK24_001658"
     CDS             complement(13344..13928)
                     /gene="clpP"
                     /locus_tag="KPK24_001658"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG2299335.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(14078..15364)
                     /locus_tag="KPK24_001659"
     CDS             complement(14078..15364)
                     /locus_tag="KPK24_001659"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG2299336.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(15558..16028)
                     /locus_tag="KPK24_001660"
     CDS             complement(15558..16028)
                     /locus_tag="KPK24_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2299337.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(16028..17377)
                     /gene="rph"
                     /locus_tag="KPK24_001661"
     CDS             complement(16028..17377)
                     /gene="rph"
                     /locus_tag="KPK24_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG2299338.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKIIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(17537..19561)
                     /gene="ligA"
                     /locus_tag="KPK24_001662"
     CDS             complement(17537..19561)
                     /gene="ligA"
                     /locus_tag="KPK24_001662"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG2299339.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(19747..20685)
                     /locus_tag="KPK24_001663"
     CDS             complement(19747..20685)
                     /locus_tag="KPK24_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG2299340.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(20787..21209)
                     /locus_tag="KPK24_001664"
     CDS             complement(20787..21209)
                     /locus_tag="KPK24_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG2299341.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            21397..22680
                     /locus_tag="KPK24_001665"
     CDS             21397..22680
                     /locus_tag="KPK24_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG2299342.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(22758..23273)
                     /locus_tag="KPK24_001666"
     CDS             complement(22758..23273)
                     /locus_tag="KPK24_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2299343.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNLDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(23273..24649)
                     /locus_tag="KPK24_001667"
     CDS             complement(23273..24649)
                     /locus_tag="KPK24_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG2299344.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(24674..25225)
                     /locus_tag="KPK24_001668"
     CDS             complement(24674..25225)
                     /locus_tag="KPK24_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2299345.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRQCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(25283..25498)
                     /locus_tag="KPK24_001669"
     CDS             complement(25283..25498)
                     /locus_tag="KPK24_001669"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299346.1"
                     /translation="MQIHRELSKSENEYMCTKKEEQRYSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            complement(25467..26300)
                     /gene="fdhD"
                     /locus_tag="KPK24_001670"
     CDS             complement(25467..26300)
                     /gene="fdhD"
                     /locus_tag="KPK24_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG2299347.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(26349..28493)
                     /locus_tag="KPK24_001671"
     CDS             complement(26349..28493)
                     /locus_tag="KPK24_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(28077..28079),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HBG2299348.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(28744..29802)
                     /locus_tag="KPK24_001672"
     CDS             complement(28744..29802)
                     /locus_tag="KPK24_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG2299349.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(30013..30672)
                     /gene="cprR"
                     /locus_tag="KPK24_001673"
     CDS             complement(30013..30672)
                     /gene="cprR"
                     /locus_tag="KPK24_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG2299350.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT         9328 a         5573 c         3328 g        12645 t
ORIGIN      
        1 tattttattg aatagttatt tatgtttatt ggaatgtatt gtgttatttc aaattcaatc
       61 atgcttaata tgtctctttt tttcttagta tttagaactt caacatttct aatgactata
      121 tcctgaattt gaatgttaaa gtataaattt ttatataata tttttgattt accaatctcc
      181 tcttttacaa atctaactac atcttctata tatttaatct tctcaatcga attactatca
      241 ttttttatat caaagttaat ctcttttata attgatttat ctataaaaac ttttttatga
      301 ctttttctta aaaatacaag ccttatataa tcatcattta tttctaagtt tagaattttt
      361 ttaattatag ccatctcctt cttaaataaa aataatttta aaacttacag caatcaatat
      421 aaaatattat tttggataaa attgcttaga atttatattt acgcttatat ctgttccgct
      481 atcatttatg acaagttcga atttacctga tgtactctga ggaactggaa cagtatttag
      541 gtatcccttt tccttcaatg tctctacact tagagagtct attgtctttt catcatcatt
      601 aattgccatg ctagctgcgg ttactatatt agcagcattc gtatagtctg catttaattt
      661 ggcactttct tgaaccttac tatacttaac taatgctata ctagatataa cgcctaaaat
      721 agttactact acaatcattt caacaagtgt aaatcctttt cgttttttat ttatcaaatt
      781 tatcaattaa atattctctc cttttcaaaa gtttcatcta ataaaattta tttttctctc
      841 taaaaattag taattgcatc aaacatagga acaaccattg ctaaaataaa agtaccaata
      901 attaatccca taaatacaat tagcataggt tctataaatt tcattatttg ctctattttg
      961 ttatttaatt cattttcata aaatttattt attacattta gacttttatc tagtttccca
     1021 ctttcttctc ctgtatttgc cattgaaata aataattttg gaaaaacatt tgctaaattc
     1081 aaagatttac ctatgctatt tccttttttt atataattat tggaaatctt taacctttca
     1141 taaataaatt cgttgtctat aacactagct gacatgtcta tagcttccac aatttgtatt
     1201 ccactgctaa ttaaaacgga caatgccctt gaaaatcttg ttgtagctac taactgatta
     1261 atttttttta taaaaggtat tttgaatttt aacgaattaa tcaatttttt tatattatca
     1321 tttgttataa agaaataaat gcttcctatt gaaaacaata taaaactaaa tattatataa
     1381 gctaagttat ttgtaaccac tgtagatatt cccattaaaa ctctagttat taaaggtggt
     1441 tcaattccat tgttggtaaa aaccacctga aaattaggta ttacaaatac gaatataaac
     1501 aacattgata agatagataa tattataagt attattggat atattgaaat agttattatt
     1561 ttcattttta atttgtattc tttatcataa taattagata aatcactcat tattatatct
     1621 aagtttccac tagtttctcc tgcttttatc atacttataa agaatttaga aaatatacct
     1681 gttatttgaa atgattctgc tattgagtta cctttttgaa tatgatttga tactatacta
     1741 agcaaattct ttgctttttt acttgactga ctctcaatga tttcaaatat ttttgtaatt
     1801 tcacatccag attccagcaa tatgcctatc tctttgcaaa ttactctaat ttctttatca
     1861 ctgattctat ttaatactaa ataaaacttt tctaaattat aaaatatatt tttagataat
     1921 ctcatgtact ataatacctt ccaaattata ttaattacaa ttaaataaac ttaatatttt
     1981 ctaatttaat gcatttaatt ttatttcact taaatataat ttatactcaa acatcttaat
     2041 taaaatagat taaataaaga caactgacca aacgaagttt aactatacaa ttcagttaaa
     2101 tcatttcatt tactactaaa cactcttcta atgtcgttat tttttcattt attagtcttt
     2161 tacaactatc ttcaaatgtt atcattccat tattttgtgc tacatcttta atagtactag
     2221 aatcctccat atttcttata catgtcttta tatcatcatt aatttctaag atttcataaa
     2281 ttgctgttct tccaaagtat cctttatcat tacatttttc acaccctaca gctaccttag
     2341 tatttacatc tccactaaaa ttatctttta tcataatctc atggctacaa tgattgcaga
     2401 cttttctaac caacttttga gatataacac ctataagaga agcatttaaa agatatggtg
     2461 gtatttgcat ttctagtaat ctagctatgg aagatactgc atcattagta tgtaaagtac
     2521 ttataactaa atgacctgtt atagctgctc ttactgctat cttagctgtt tctgcatctc
     2581 ttatttctcc gaccattatt atatctgggt cttgtcttaa tatcgctcta agacctacat
     2641 caaagctcaa ccctactttt gaatttactt gaatctgact tatgccatcc atcttatact
     2701 ctacaggatt ttctatagtc ataatatttt tatttattcc ttttaaatca tttaataagg
     2761 aatatactgt tgtagtcttt ccactacctg taggacctgt cactaataat attcctgctc
     2821 ttttatttat tatacgattt atagatttta tagcttcctc tgaaaaacca agttctaatt
     2881 tgtcttttaa aaaagtatct ctattaagta tcctcaaaac tattttttca ccatagacag
     2941 ttggtattga tgaaactcta atgtcaatta aatttccacc taactttata tccaccctac
     3001 catcctgagg aagccttttt tctgttatat ccattgatgc ctttaatttt ataaccgtag
     3061 acaatgatgg atagacatca actaaaaact ttgatacttc ctttagttct ccatctaccc
     3121 tcattctcac tattaaatgt tctttaaatg gttctatatg tatatcactt gcgttttctt
     3181 ttatagcttt ttctaaaaga ttttcaaata atttttttgc ataatcctca tcaagttcaa
     3241 agctattata atatttttca atcttttcac ttataagttc ttttttctcc ttcttcaata
     3301 taatttcttt acctgtagct aatctcaagt cctgtagagc ataaatatcc tcatcttcta
     3361 tttctacaac taaattatca tcttgaaact caactggaaa tacattatat ttttttgcaa
     3421 gtttttctgg tattaacatt ggcaaactgc tttcctctaa tataatctta atcacccttt
     3481 atatacttta atttattagg tatattcatt tttttcatag agatattttc ttcagttttt
     3541 atgcttattt tcattggata attggtttgt atgctatcta aatatttact acctttatct
     3601 acataattag atagtttctc cttatctcca atgcacttta tttcatatgg ttgtgctaca
     3661 ggaactccat ttatatttat atggcttcct gcaagtacaa cttctgaata ttgattaatt
     3721 ctttgaccat ttatttctat gtattttcct ccgtttactt ttaattcatt tataactttt
     3781 acaagaatct tattatagtc tatactatta gctatattcc ctgttttttc atctatagca
     3841 tctatactta atattatccc actacccttt acatctgaat atgatagact atctctaagt
     3901 gtttctattt ctgatatttc ttcagaattt ttttgatttt gtttcattga attaatttct
     3961 ttatctaact tgtctttctc tttggttaat tcttttatag attttcttgt catttttatt
     4021 tcttttctta ttgacttgtc tttgcttgta taaactgttt cttcattgct ataatctcta
     4081 aatatggttc caacaagtat acctaaaata gtacttgcta gtattataaa cacatattgt
     4141 tttttcatac tgtattgctc cttacaaatt tatttttata tgataatttt ctttaattaa
     4201 atattaaaca tttacatatg cttattatat caaagttata tattaaaatt catctttagt
     4261 atataatatc atacattgaa ctcctatcac taatttacca atatcaagta tgcattttta
     4321 gatgttctta aaattatata aactttcaat aaacattgtt ttgatattat aaaaaaacat
     4381 aaattaggaa aggaaataat aattatgaaa aacaaataat tgctttggag attattaata
     4441 tcatttttta gcaaaaaaaa gtaaaatggc atagcctcaa agctacacca atttactaaa
     4501 aattatatca ttattaacat taattttaaa catatacgtt tataagtttt cctcatattt
     4561 taaaattctt ttaattaatt tatctcttaa tttccctact tcatcaaatc tcatattagg
     4621 actatagtaa ctttctataa tatcatgttt agacttggct ctcttaagat ttagtactgc
     4681 ccaacaaatt aaatcatcta aaactttttc atcaaattta atatcttctt catatttaag
     4741 tagtttttct ctatttaaaa actgatttaa atcaattgta tcttcttctt taaaagtcgt
     4801 acttatagtt atgcctatat ctaaatcagt taatagtaca gtttcaattt tctctggaat
     4861 aagtggataa tgataaactt ctacatttaa acctttttca atagctttct tatatacttt
     4921 gtttaataat gtagttttcc cagttcctat ttctccatct aaataataga ctttctttac
     4981 atcttttaaa atgttttctg tataatctaa acaccctaca ggagttatag ctgaaccaaa
     5041 taaatgcttt tctcttttta gatttcctgt gcttttaact ccatcaaata acctttttat
     5101 aaattcttct gtgaccaaat ttactttgcc ataatccata caatcagaat atttttcttc
     5161 tatgtctcta aagattggtt ctgctgattt taaaaactta aatgctcttt taaatgaatt
     5221 actaatatct ttatcataaa ctgatatttc ttgtttattc ttttctaatt cttctgtatt
     5281 ccagtaatca cctaaattaa ttatctcatc caaagctcca ggtagctttg ggtccatagt
     5341 atgtggggca gttgcatcca taagtaccac gcctaatttc tttataacta aaccatccaa
     5401 cgaacttggg tcagaaggac aatgatataa atcaatatca tatcctctat gcaaaaactc
     5461 tttataaatt tttttcataa gtgaggattt tcctactcca ggtccaccct ttaaacaaaa
     5521 aactcttttt acattttcag gaattatata gtcaaaaaaa gaatatgagc cttgtgatgt
     5581 atttccaccc gggaatgcct tctttacctt tccttccata ttagcgcctc ctcataattt
     5641 acaatatctc ttaattaaga tattcatatt ataagaattt tattacagct ttctgatttt
     5701 gacaaattta aaacttgcca tctaatacta ataacaggtt aattttatct aattattata
     5761 gattctcata ctgtgcctta ttctatctat aaatacaatt taagtgtcca tattgaaata
     5821 tttgtattgt aatacagctg gatattactt aaatccaatt gtttccatta taattttatg
     5881 ttaaaataat attacaaaat acatctgttt ttcttcataa acgggtgaaa ttacctatcg
     5941 gcggtaaaag cccgcgagcc ttatggcata atttggtcat attccaaagc cgacagtaaa
     6001 atctggatgg tagaagaaaa tagtatatga gtacctttat gtaattttac atgagtaatc
     6061 tatacaaatc cttcaactac cgtatttatt catgaaatta gacacattca aggtacctaa
     6121 tatacaggtg ctttttttgt tgtttatttt acaattatat cgtacttata aaatctatta
     6181 agattggagt gttatcatga aacaaaaatg gatagtattg attatcatct gtattggtgt
     6241 atttatgtct actcttgatg gaagtatact aaatatcgca aatcctacaa ttgctgctga
     6301 ctttaaaatt aacatgtcac aaattcagtg ggttgtaaca gcttatatgc ttgttgtaac
     6361 agctactatg ttatttttcg gaaaacttgg cgataaagta ggttcaaatc gtctatacac
     6421 attaggattt tttatattta caataggttc ttttctgtgt agtatgtcaa acaatttatc
     6481 aacattaatt tcttcaagaa tctttcaggc agttggagca agtattttaa tggctactgg
     6541 gcttggtata gtttccaatg catttccagc aaatgaaaaa ggaaaagcta taggaatcac
     6601 tggagctgta gttggtatag gaaatatgag tggtcctgta attggaggta taattttaga
     6661 acattttgga tggccttcta tttttattat caatatccct attggtatta tagcagtctt
     6721 tcttggcatt aaatttctgc ctaaacctgt tttggatgaa caaaacaaga gctttgatat
     6781 tccaggtctt ctacttttcg catcttgcac tacgcttata ttacttgcaa tgaatgagaa
     6841 gggtaataca agattgtatt taggtataac agcacttata atcttcttat tgttggcatt
     6901 aagagaagta aaatttgaac aaagctttat tgacctacct ctatttaaaa atagaaactt
     6961 tactgtaggc aatatcattg gagtagcttg ttattttcct caaatggcag tatcttttct
     7021 acttccattt tatttagaac aattaaaaaa tttatctcct atgatggctg gttatgtaat
     7081 gactgtacat ccacttatta tggtacttat agcacctatt gcaggttcac tttctgataa
     7141 acacggtgca aaaaacattc taacagcatc tttttcattt atgaccatct cactagttgg
     7201 aatggcactt ttaaaagcag attctccatt gtacctttta attgtatgtt tagttatttt
     7261 tggacttggg ctaggagcat ttagttcacc aaacaatagt agtattttag cagatgttcc
     7321 tcctcaaaaa caggggtacg gaggcagttt tcttgcaact attcgtaatt tatcatttgc
     7381 tcttggaaca gcatttttta gtagtttttt tgcacaaagt cttacataca atcaaaagtt
     7441 taaaagtcac acatcagcct atgttatagc tagcaatcag tcttattgga ttgcagcatc
     7501 agtatgtttt gtagggctta tactaactgt tttctttatg aggaaaacag ataaatctat
     7561 ttcttaaatg ctatttatta ctgactacat aaatcaataa ttttttagtt taactatttt
     7621 atactaattt tcccatacct tactaaaatt tattagtatt tagcaaaatt actgatattt
     7681 attgtttata aacaataagg atgccaaaaa aaagttcatt aaaactattt taaggcatcc
     7741 tatattttac tttaatatta atataattaa aactaactat tattacattt attattcaaa
     7801 taaatcttat gtcaatattt aacattttaa atttaatcta ctgttatttc atatccatgt
     7861 tgattgtatt gatttacaat ctcttcccat aattcttcaa caccagtctt ttttagtgaa
     7921 gaaacaggaa ttactttttc actactttcc atttgcaatt tttttcttat tatacttatg
     7981 tgtttttgat attgtcctct agatatttta tctgccttag ttgcaataac tacacagtta
     8041 tatccaaagt gctttatcca ttcatacata agcttatcat ctgctgtagg ctcatgtcta
     8101 atatctacaa gtaggaatat agaacataac tcttgtctac tttctagata tcgttccatt
     8161 atacctcccc acttttcttt ttcagactta gctatctttg catatccata gcctggaagg
     8221 tctacaaaat aaaattcatt atttattaga taaaaattta tagttctagt ttttcctggt
     8281 gtttgactgg ttctagcaaa gtttcttctg ttaagcaatg tatttattat tgatgatttt
     8341 ccaacatttg acctaccagc caatgctatc tctgggattc cttctgctgg atactgtgat
     8401 ttattcacag cactcatagt tatctctgaa cttctaattt tcattatttt caccactctt
     8461 taataaagct tgttctaata cttcatccat atgttctaca agcacaaact ccattttttc
     8521 ttttacattt tctggtattt catctaaatc agcttcacat tctttaggaa ttaatacttt
     8581 tgttatacca gctctatgag ctgctaataa cttttcttta actcctccta ctgctaagac
     8641 tctacctctt aaagttatct ctcctgtcat tgctatattt ccaggcacag gtcttttagt
     8701 aagtgctgag attactgcaa gagccatagt tataccagct gatggtccat cttttggaac
     8761 agcaccttct ggaatgtgta tatgaatatc attcgtctta taaaattcag ggtctatatc
     8821 aaacttgtcc actattgacc ttatataaga gatacctgtc ttcgcagatt ccttcataac
     8881 atctcctaat tttccagtta atacaatttc gcctttacct ttaagtacgt ttacttctac
     8941 ttcaagtgta actccaccaa cttctgtcca cgcaagacca tttactaatc ctatttgagg
     9001 gttaacttct gcaagttgat acttaaacat gtcttttcca agatatgtct ctaggtcaga
     9061 cttatttata acaacctctt caagagttgg gtcttcaacg tattttttgg caacctttct
     9121 acaaatctta ccaatagttc tttctagtgt tcttacacca gcttctcttg tataatggtt
     9181 gattatactt cttaaagttt catcatctat ttttataaag ttttctttta aagcatgttc
     9241 tttaatttgt tttggaagta ggtatttttt tgctatattt aatttttctt cttctatata
     9301 tccagatact tctattactt ccattctatc taataatggt cttggtatat tacttagact
     9361 atttgctgtt gtaacaaata gtatttttga taaatcaaaa ggtatttcta agtaatggtc
     9421 cacaaaatcc ttattttgct ctgggtctaa tacttctagc attgctgaag ctgggtcacc
     9481 tttataatct gctgccatct tatctatctc atcaaataag aatacaggat tttttgtttg
     9541 ggcttctttt accccattaa ttattcttcc tggaattgaa cctacataag ttcttctatg
     9601 acctcttatt tctgcttcat ctctaactcc acccaaagat attcttacaa attttctatt
     9661 taatgctctg gcgattgatt ttactataga tgtctttcct gttcctggag gaccaactag
     9721 acatattata ggccctttaa gtgacttagc aagagttctt atagctagat actctagtat
     9781 tctctcctta actttttcta atccataatg gtcttcatct aatatatctt ttgccttagt
     9841 tatatctaat ttatcttttg tctctttatt ccatggcaat gagaatatag tatctaaata
     9901 gtttctactt acagatacat ctggagacat agaagatatt tttgaaaatt tatctatctc
     9961 tttttctatt ttctctttag ttgtcttagg tgcttttatt tttttaagct tttctctata
    10021 ctcatctgct tcagagttta tatcttcttc ttcacctaat tctttctgta tagcttttaa
    10081 ttgttctcta agataatatt ctttttgaac cttattcatc tgttttttta ctcttaaagt
    10141 tattttcttt tctattttta atatatctat ttcctctaat aatatagaat aaataagttc
    10201 aagtcttttt ctaatatcaa attcttctaa tatttcttgt ttttggcttg attttagata
    10261 tatgtttgca gctatcgtat ctataaatct atctacatct tctatatcag ctaaagatat
    10321 taatatttct ggtgataccc tatttccaat atttatatac tcttcaaagg catcaaatac
    10381 atttcttaca aatgcttcaa tttcaacttc tgtttcacta tccaaattat cactatcaaa
    10441 tactatttct tctattactg ctctgaaata cccatcttct tgttctattt tctttactct
    10501 accccttgat acaccttcaa ccaaaacacg aactgtatct cctggtaatt ttatcatttg
    10561 cttaacttta cagattgttc ccacatgata aaaatcctct tctcctggct catctacctc
    10621 tgcttctttc tgagaagtta aaaatataag ttcttcatcc atcatagctt ggtctaaagc
    10681 tttaagagat atctctctac ctatatcaaa atttaatatc atatatggaa atatagctaa
    10741 tcctcttaga ggaattaatg gtaattcatg atctatttta gtataatttt gttccatcaa
    10801 cattactcct tttctagtct aattgctttg cttttaatat tatatatttg aacaagttaa
    10861 ttttcaacat taatgataaa ttcttagaat actatcatac tatattatct ttttattatg
    10921 atataatatt aatattagta tacttagatg ttatttatct tttggaggtt tctattatgt
    10981 tgaaattgtt tttaaccttt ttaaaaattg gtgcgtttac atttggtggt ggatacgcta
    11041 tggttcctct tatacaagat gaacttgtaa acaaacaaaa acttttagag caggaagaat
    11101 ttattgatta tttatccata gctcaaagtt atcctggagt actggcagtt aatatatctg
    11161 tattattagg atataaactt tatggtatac caggagttct tatatgtacc ttaggctctg
    11221 ctttaccatc attttgtata gtcttagtct tatcgtatct atactttaaa aattcatcat
    11281 ctaaaatttt agatggattt tttaaaggag ttgcgcctgt tgtaatagct cttatattgt
    11341 attcttttac gagcatgttc aagaaactac caaagtctaa aactaacata ctactattaa
    11401 ttatagcagt tttatctgtt ggtgtattca atataagccc tatttattta ataattggag
    11461 gaggtgtata tagtctatgt cagaaataat actactcttc ttaatattcc ttaaaatagg
    11521 ggcatttagt tttggtggcg gttatgccat gctaccattt atccagcaag aatttatatc
    11581 taaatatcac tttataacta atcaagagtt tttggattta cttgctatat cacaatctac
    11641 accaggtcct gtagcaataa atagtgctac atttgtagga tttaagactg ctggcttctt
    11701 tggttctcta gccgcaacta ttggtgttac aatttttgca ttaataggtc taactattat
    11761 atcaaagtta ttttctaaat ttaaaaataa taaaaaggta gaaaacttat tgacagttct
    11821 tagaccaatt actctaggat tcatattatc agcagctttt tcatctgcca aagaagttaa
    11881 ttgggattta tatggaataa ttgcatttat agcatcattt tatcttttat ataaaggaaa
    11941 aattggttcc attgcaattg tattcgtctt tggtattcta ggtatattac tcacattttt
    12001 ataataatga tacctatttt atatttagca tattaacata aaaaaaggag cttttaaagc
    12061 tccttttcat catcttatgc actttcctct tgatccttaa gtactatgat aggatttact
    12121 gagtcttcgt tcacagattt ctctgttact ataaccttct ttatattatc tctagatggt
    12181 acttcaaaca tagtttccat cattacgctt tcaactatac ttctaagtcc tctagctcct
    12241 gtatttcttt ctatggcctt tttagcgatt gcacgaagag caccttcttc aaattctagc
    12301 tcaacatcat ctaactccaa taacttttta tactgtttta ctagagcatt tttaggttcc
    12361 tgtaatatct gcattaatgc atcttcatcc aacaactcta aagtagctaa aactggtatt
    12421 cttcctataa actctggtat tataccatat tttaataaat cctctggaag tactttttca
    12481 tatagcttcc ctaaatctaa ttctttttta ctttctattt tagctccaaa acctagtgtt
    12541 ttatctccac cacgtttttg aattatcttc tctaatccat caaaagctcc acctaatata
    12601 aataatacat tagtagtatc tatctttaaa aattcttgat gaggatgttt tcttcctcct
    12661 tgtggtggaa catttgcaac agtaccttct aatattttta gaagtgcttg ttgaacacct
    12721 tcaccactaa catctctagt tatagatggg ttttcagatt ttcttgcaat cttatctatc
    12781 tcatctatat atattatacc tctttcagct ttttctatat caaaatcagc agcttgaata
    12841 agttttaaaa gtatattttc aacgtcttct ccaacatatc cagcttcagt caatgatgta
    12901 gcatctgcca ttgcaaaagg tacatttaga gttcttgcta gagtttgagc aagtaatgtc
    12961 tttccagaac ctgtaggtcc taataaaagt atattactct tttgaatttc tatatcctta
    13021 ctactagatt ttttactgta tattctctta taatgattat aaaccgcaac tgataaagct
    13081 tttttagctt tttcttgacc tattacataa tcatttagaa tctccatcat ttcctttggt
    13141 tttggtaaag atgtagtatc ctcatctata gtatcttcta tctcttcttg aataatttcg
    13201 tcacaaagtt caacacactc gtcacaaata tatacatttg gacctgcaat taatcttctt
    13261 acttgatctt gatttttacc gcagaatgaa cactttaatt gtcgtttttc ttcatattta
    13321 gacatattaa cacctctctc tatctacggt ctctttgtaa atacttcatc tattaaacca
    13381 tattctttgg cttctagtgc actcatgaag ttatcacgtt cagtatccat tttaattttt
    13441 tctaatggct gaccagttct ttcagataat atctcattta aagtttcttt tatttttaaa
    13501 attctctttg catgaatttc tatatctgtt gcttgacctt gagctccacc taatggttga
    13561 tgtatcatta tttcactatt tggtagtgct agtctttttc cttttgctcc tgctgccaac
    13621 aagaatgctc ccatagaagc agccatacct atacatatag tacttacatc tggtttgatg
    13681 tattgcatag tatcatatat agccattcca gaagttatac ttcctcctgg agaatttata
    13741 tataaatgta tatctttgtc tgggtcttca gcttctaaaa ataatagctg tgctactata
    13801 agccctgctg tggcatcgtt aacttgatct cctaagaaga ttattctgtc ttttaatagt
    13861 ctagaaaaaa tatcataaga tctttctcct cttcctgttt gttcaacaac tacaggtact
    13921 aatgccatgg tttcataccc cctttattct atataattta cagtagcttt ttttaagcta
    13981 ctgtaaatta ttatacgcat ttaactaaat tatatttgca catcattata tactaaacat
    14041 ttcataacgt caactatatt agttagcgtt tattttatta agctattgtt gcattatcaa
    14101 ctaataaatc tattgttttt cttattttta tttgaccttt tatatcttct ttgtcggcat
    14161 ctcttaaaga agcttctatt ttttctactt ccatattgta agcagaagcc attttttcta
    14221 actcagcctt aaattcttct tctgttgctt caattccttc agctttagtt attgcttcta
    14281 atactaaaga agacttaact aattttttag catcttcttt tctctcttct cttaattctt
    14341 ccatagttct accagtcatc tctagtaatt gttgtaatcc aaatccttga tattgtaatt
    14401 ggtagttaag ttcatttaac atattatcta tttgatgttg tatcataact tctggtattt
    14461 ctatttctgt attttcagca actttttcta ctactgaatt tctagtttct gcatctgctc
    14521 tatttttagc ttcttcttct aacttagctc ttacatctgc ttttaattca tctaaagaat
    14581 caaattcact agtatcttta gcaaattcat catcaagtgc agataattct tttactttta
    14641 catcatttat ttttacatta aatacaactg gttttccagc taaatcttgt gagtgatatt
    14701 cttctgggaa agtaacatta acttctactt cttcaccagc ttttttacct actaattgtt
    14761 cttcaaatcc tggtataaat gtgtttgaac ctataactaa gttataattt tcacctttac
    14821 caccatcaaa agctacacca ttttcaaaac cttcgaagtc tattatagct gtatctccat
    14881 cttctaaagc tttgtcttct acacttacta atctggcatt tttttcaacc atttcttgta
    14941 gtttagcatc aacattttcg tcacttacag tattctcaac cttagctatt tctataccct
    15001 tgtagtttcc taattcaaac tctggtttaa cttctacatt aagaaccata accaacccat
    15061 tgtctttact tatttcttct atatctaaat ctggattgtc tattgggtct atgtctaatt
    15121 ccttgatagc ttctggataa acttctggga ataatatttc tatagcatca ttgtagaata
    15181 taccttttcc gtattgagtc tctataacaa ttcttggagc tttacctttt ctaaatccag
    15241 gtatattgta tttatttctg cttttattat aagccttatt tactgctgct tcaaatttat
    15301 tgttatctac tgttattttt aaagtaactt tattaccctc ttttttaatt agttctgctt
    15361 tcatccttct aatcctccta gtgatattta tgtaataccc gataacggta ttataattca
    15421 ttctatttat aattcaacca cttaattata tcacataagt ttttttattt acaagtgttc
    15481 cattaatatc gttgtactta tttgataaaa tttaattttt tgagcgtttt ttgccatttt
    15541 tttagataaa tacaccttta tatgcgtatt tcttcaattt ttatgtcatc ttcttctata
    15601 tccataatga caaaactttt atacgatact cctcttggaa gtgaagtgct tccaggattt
    15661 atgtaaagta caccatcttt aatttctcta aatggggtat gggtatggcc aaatataact
    15721 atatcagcat caacttcagt tgcctttttt tctaacatat gagttccata ttttacgcca
    15781 tacttatctc catggcataa gaatatggtt ttatttgcca cttcaaagac aacttcttcc
    15841 tcaacagcat caaagtcaca attaccttta actgcaataa tacctacatt agtcatgcta
    15901 tgaatatatc ttgaatcagt aaagttatct cctgcatgaa ttattaggtc acattctttc
    15961 aaatatggta ttgttttttc catattttta atcatcatat gtgtgtcgct tactatgcct
    16021 attttcatta cttcactacc ctttttataa actcttgttt cataagtttt agtgcgtttg
    16081 ctctatgact gattgaattt tttataacac taggtatttc accaaaagtc ttatcatatc
    16141 cttttactat aaataagcta tcatatccaa aaccattttt acctttttct tcaaatccta
    16201 tcattccttc acaaattcct cttactacaa attcttttcc atcaggaaaa actactgcta
    16261 ttgcagagac gaatcttgca tttcttttgc tcattggtat attttgcata gcttttaaca
    16321 gtttttttct attttcttca tctgttgcat gttctcctgc atatctagct gaataaacac
    16381 caggtttttt gcctaaagca tctacttcta agccagaatc atctgatatt gctataagtt
    16441 tagtcttttt tgctattgtt ctagctttta tcaacgcatt atgctcaaat gtttttccat
    16501 cttctactat ctctatacct gctaaatcaa catcttttaa agagtacact ttatattcaa
    16561 aatcttttaa gatttcacct atttcttcta gcttatgagc attattagta gcaatcacta
    16621 cttcattacc ataatccata cccaatatct catctgatat ttcacctaaa gcttctcttt
    16681 gaagccttat taattcttta ttcccttttt cacctaactc taacaataaa tttaaatctg
    16741 ctctcgaaaa aggtttctct tctcctgtcc cctgtacttc tacaaactca cacttatcag
    16801 tcattattat attcatatct acctgtgctt tagaatcttc ttcataacat aaatctagca
    16861 tgtgtattcc atttacaata ccaacactaa ttgcagacac aaaatttgtt attggcatct
    16921 cttttattat ctttgcttta tataatttat atattgcttc tgcaactgct acaaatgccc
    16981 ctgttataga tgcagttcta gttccaccat ctgcctgaat cacatcacaa tctacccaaa
    17041 tagttctttc tcctaattta ttcaagtcta caacagacct tattgctctt cctattaacc
    17101 tctgtatttc ttgtgttctt ccatcaattt ttcccctaga tgcatctctt atctttcttt
    17161 gttgtgttgc tcttggtagc atagaatatt cagcatttat ccatccagta ccactatttc
    17221 ttaaaaaagg aggaactttt tcttccactg atgcagtgca tataaccttt gtctctccca
    17281 tttctataag aacagaacct tctgcatact ttgtataatc tctagttatc tttacaggtc
    17341 ttatctgttc gttacttcta tcataacttc tggtcatttt ttatatcctt tctcatattt
    17401 tttatttttt ataaatttat ttttataatt tttaattttg tcaaatatta attattgtca
    17461 tttttcgtaa tcttattagc ttactgaact attctatcat taattatagt agttttctac
    17521 taaattaagc gactttttaa ctctctaata cagcttttac ttcatctttc gatgataact
    17581 ttaataattc taaaaatctc tcttcatcta taactttaat tcctaaatca tttgctttag
    17641 taagtttaga accagcttct tcacctgcca aaacaaatgt tgtcgattta ctaacactag
    17701 atgtagcttt tccacctctt ttttctatca tatcttttgc atcatttctt tttaatgtag
    17761 gaagtgtacc agtcaatacg attttcatcc cttcaaatat tttttctact ttttcacctg
    17821 tattatccaa tgattttgta tttactccta cattttttaa cttgtttata acgttcatat
    17881 ttttgtcttc cttaaaaaac tcgacaacgc tgtttgccat tatacttcca aattcctcta
    17941 attcttctag ttgctcacta gttgcagaaa ttacttcatc taaatcctta aagttatatg
    18001 caagtatctt agctgcttta actcctataa atttaatacc taatccattt ataagtctcc
    18061 ataaatcatt ttcttttgat ttttcaattg aatttattaa attgtcagca gatttttcgc
    18121 ccattctctc taggtcgact acatcttcct tttttatata atataaatca gatacatctt
    18181 gaataatttt ttcatttaat agcagtgtaa ttatagattc tcccaatcca tctatattca
    18241 ttgcatccct tgacacaaag tgtataattc cccttcttat ttgggctggg caagacatat
    18301 tgatacattt tacagcagct tctccttcta atcttattgt tggctctgaa caaactggac
    18361 acttttctgg cattttaaaa tctatttcat ctccagtacg ttcttctttt ataacttcta
    18421 aaacttgagg tattatgtcc cctgcttttt gaactaatac tgtatcattt atttttatat
    18481 ctttttctct tatataatct tcattgtgaa gtgtagctct acttacagta gtacctgcaa
    18541 gtctgactgg ttcaagaatt gctgaaggtg ttattgtacc agttctccca acttctacta
    18601 ttatatctac tattttagtt ttcttctttt ctgctgggaa tttatatgca attgcccatc
    18661 taggactttt ggctgtatat cccatttcgt ctctttgttt tatattgttt acttttatta
    18721 ccataccatc tatttcgtag tctaaattac ccctgttatc tgtccagtat tcaatatgct
    18781 caataacttc atctatactt ttacaaactt tataactctg acttactgaa aatcctagtt
    18841 tttctaagta ctctagtgat tcactgtgac tttctaaatc gtattcttct atattttcca
    18901 tattaaatac aaatatatct aatggtctct ttgctgttag ttttgggtct aattgtctca
    18961 atgaaccagc tgcaagattc cttggattag caaataatgg ctgttcttgt tcttcttgtt
    19021 gttcatttac ttttctaaaa ttatcttttg atatatatac ttctcctcta actattagtt
    19081 cttttttatc atttatctta agaggtatgc tttttactgt cattaagttt tgagaaatat
    19141 cttctcctac aactccatct cctctagttg ctcctttttc aaactctcca ttgttataag
    19201 ttagacctac agaaagacca tctattttaa attcaacaac atattcaaca ttttctccca
    19261 catattcttg aactctctta tcaaaatctc tcaaatcttg ctctgaaaaa gcatttgata
    19321 aactcaacat aggagtttta tgtacaattt gattgaactt atctaatggc ttacctccta
    19381 ccctatttgt tggagaatca attctcttta gttctggatg ttcttcttct aaagaaatta
    19441 actccttcat taacttatcg tattcataat ctgatatttc tgggctatct tgattataat
    19501 acctttcatt atggtagttt attgtctcta taagtttatt aatttcatct tttacgctca
    19561 ctttttgcac ctcttcatat ttttaaaatt aaattatttt gaaatttccc tataattaat
    19621 tatctcctat ttatatattt tttagtatat cactttttgg gctttaaatt tattacaaaa
    19681 aaaactctta ttagtatctt taatacgact aataagagtt tttattttta tcaaaatttt
    19741 ttatttttaa tagcctcttg atatgtctac aacattatct acaggtttat tctccattat
    19801 atagtgtttc aaattattat aaaccatatt aaatgttctc tctttgtttt tatctgacac
    19861 ccaagaatta tgaggagtta ctatgacatt ttcgcactcc cacaacttat tgtccttgtt
    19921 taaaggttca ctttcaaata cgtctaatgc tacccctcta aatttctcta cacattcttc
    19981 taaatctttc tgattaactg tatttcctct acctacattt ataaatacag aaccatcttt
    20041 cataagttta aacatatctt tgtttactat tccatctgta ctttttgtag aaggcattgc
    20101 tacaactact atatcacatt cttcaaatac aatattcata tcttcagtag caaaacactt
    20161 atcaaagtat tttttactac ttccatcagt attgacaccc catatttcta caccaaaagc
    20221 ttttaatctt tttgctgctt ctgaagatat agtacctgtt cctaaaaatc caactctttg
    20281 tccatacaat tctgatacag aaaaatccat cttccattct ttattttgtt gttgtttaaa
    20341 aaattttgca ctatttttat atatttctaa aatatacaat acaatacttt caccaatagg
    20401 tacactatat ccgcctctat tattagctat cttaatattt ctcttcaata cctcttcttt
    20461 tggaagttgg tctataccta cactagttgt ctgtatatat tgtaaatttt tcatcttaga
    20521 aatatctaat ctctcaaatg gattgtatgt aactagtaca tctgctgtat ttacatcctc
    20581 attattttca atactatctt cagagtaatg tatgacatca tagcctaatt ctcttatttt
    20641 atcaaatttt tcttttccat aatttattgt aaataaaact ttcatctcaa atctcctcca
    20701 tctacccaca tttaaaattt tatattctaa aatataatat gatactcaat gtacattagt
    20761 taaaattact ataagatatc ttaaatttac ttatgttcaa ttgtttttcc taaagacata
    20821 gcattacgtt cttgaactaa attttctatt aaattattca atgtccaatc aatagatgtc
    20881 tctgttacca tagctgtaaa aggtctattt attctcactt tttttagccc ttctaaaaat
    20941 gcatttactt gtgtgcttgg agtgttatta aatattacag acttactctt aatcccattt
    21001 tcacattcat ttgatatatt tccatctaaa atgtctctta ttatattctc tccattttta
    21061 tcacttaaga tgatatggtc tcttattcca gcaaagttac ttacgttttt aattaatgat
    21121 aattcttttt tattaaagtt tacaagcata atacacttcc tgttgtgtat tttatctagg
    21181 ttattattat ttattttttc aaatgacatt ttatccatcc tttcatttat ttatatactt
    21241 taatttaccc catacattta aaataatcaa tttttatagt taatttttat attaataccc
    21301 aaattatatt taaatagttg ggctatccaa aactaatata taaaattaga ataattagat
    21361 taatatctac aaatcataac ctaaaggaga gataccatga atacattact aaacaataaa
    21421 tttagcatta aaaacttttc aaatgcttgg gttatagtac ttggttgtat gttgatacaa
    21481 gctgttccat ttagtattgt agcaagtata cagcctcaat ttataagtta tgttgtagaa
    21541 ggagaaggct ttagtttagc ttctttttct ctaatattca ctattggtac tattgcctca
    21601 gcaattgcat caccttttat aggtgtatta ttttctaaag tcaatattaa gatactatat
    21661 ttattaggtt gtataatttc tagtattgct tttgctactt tttctatatg tactgaacta
    21721 tggcaatttt acactgtatc atgtattgtt caaattggcg ctacaattat atctgctata
    21781 ggaatacctg ttttaataag tgcttggttt gatgaagaaa gcaaaggaaa agctttgggc
    21841 tttgcttttg ctggtggttc tattggtagt atatttctac aaccacttgc agttcgctta
    21901 ttagcaactc aaggatacag tcgttcttat ctaatatttg gaattatagc attaatagtt
    21961 ggtatacctg taatattatt catactcagg atgcctaaag atattaatga aaaggtaaaa
    22021 tctaaaggta aaaaagagaa agcatctgat acaatttctc cttcagtatg gggttataca
    22081 ttaaaagaag ctaaaaaaat taaatatttt tggattatga ctattggatt tatatttgtt
    22141 ggtatatatg tttcatcatt atccgtacaa tatccaactt atcttagaaa ttatctaaat
    22201 cctattctaa taggaacagt aggctccaca tttgctttat ttgctctcat tggtaattta
    22261 tttggaggat tattgtttga taaattcggt caaacaaagc cacttattat atcttttgta
    22321 ttggttctta tagcaacttt atcattacta ttggtagata aatcttctac ttttgcattt
    22381 gtatttgcca ttttgaaagg tctttctgta tttgcttata tgattggtcc tgcttattta
    22441 actggctctt tctttggtca aaaggagttt ggtagcatat atggaatcgt acaattaatt
    22501 tttgcagtgg gatttgcatt aggttcaaca ttatttggat tgttagttga taattttgga
    22561 tacagatttt cttggtctat tataatttta tcagttataa ttgcctatac tttacttatt
    22621 ataacttcta taggaatgaa aaaattgaat aaagaaagat tagataaact aatgcaataa
    22681 ttgattttta gatatataaa tattataata aaaaagatgg agcttttaat cataaataaa
    22741 actccatctt ttttatatta caatatagct aattttctct tatttttaac tttactatct
    22801 atatcctcat ccttaactat agtaaatgcc ttagttggac aaacatttac acatgcaggc
    22861 ccttctggcg attctataca aaaatcacac ttatttgctg tcattctttc tctaaagcaa
    22921 agctttcctt catctacatc ttttaatgta actatgtcta ttgcacctat aggacatgct
    22981 aacatacaag tcttacatcc tatacaattt tcttcattta tttttattgt gttcccaacc
    23041 ctaactatac caccatttgg acatgccttt gcacaaggag catcttcaca atgtctacac
    23101 tgtattggag cacttacttc ttttgtcttt atgacagaaa gttttggatt aaaatttatt
    23161 tcatctagat tttggtaaaa aatatcttca tctgaatgtg caactacaca tcctatcata
    23221 caagttctac atcctatgca tttatcaggg tctgctataa caaaatagtt catcacttag
    23281 ccacttcctt tcctatacaa tatgtagtgt gtaatagatg atgagatttt tctcctaatg
    23341 gttctaccag atattcatca tacagttttt taatatcagg attctcatga gattttctaa
    23401 ttgctaaatg ttcatcatgc acgtatgttg cttttgttct attttcataa gccaattcct
    23461 tatattcttc tagtaaaatt tttggttgac ctccaccact tacacaccca acaggacaag
    23521 tcataacttc aataaaatct acatctaatt ttcctttttc caagtcttca agcactggta
    23581 ttacattttt taatccactt actactccaa ctcttaagtc taagtctcct attttaatag
    23641 ttgactttct aaaaccattt tctcctctta cttgttttac ttctacatca ggaatacttt
    23701 ctccagttat aagctcatat cctgttctta tagcagcttc cataacccca ccagtaacac
    23761 caaagatagt tccagctcca ctataactac ctaaaggact atcaaatttt tcttctctta
    23821 aatttttaaa atcaattccc atgtctttta ttaaataagc tagttctcta gtagttaata
    23881 ctacatctac atctctatat ccactagaat ccatttcttc tctatcacat tcatattttt
    23941 tacatgtaca tggcattata gaaacactaa atatctttga tgcatctaca ttatttatct
    24001 ctgcaccata agttttaaat attgcaccac ccatctgctg aggtgattta caagaagata
    24061 aatgattagt taatttagga taattttttt ccatgaattt tacccaagct ggacaacaag
    24121 atgtaaacat tggaagagtt ccaccttctg tcactctttt aattaattca ctaccttctt
    24181 ccattatagt taaatctgct gcaaaattag tatcgtagac tctatcaaaa tttaaagctc
    24241 taagtgctgc tgccattttc ccagcaccta agtatcctaa ataatatccg aaatcttctc
    24301 ctaaagcaac tctaactgct ggtgcacatt ggaccatagt gaatacattt gggtcactta
    24361 aagctttctt caccttatct atatcacata cattgtatgc tgcaaatata ggttcattta
    24421 tagactctgg tatctctctt tcttgttttt tctcttgtaa aaactcaaat ccttcatcta
    24481 taactgaagc ataagattta caagtttgaa cacattgacc gcacataaca catctatcta
    24541 aatttatctc ttgtggcttt ccctcttcac cttcaatagc atttactgga cagacctttg
    24601 aacattctct acatccagta cataaatcct tatctatatt tattatcata aacgtcacct
    24661 acccctgtat cctttattta tttgttaaca ataaactaag tgcagcttgt ctattcttag
    24721 cttttttgtc atctattgga gtaacaagtt ttaatgcttc ctgaggacat gcacttatac
    24781 aagctatttt ttcattgtct ttacataagt cacatttata agctatcttc ttattttcat
    24841 ctaaaattac ttgttctact tctttaccat cttcatattg tggaagtaag tctatagctc
    24901 caaaaggaca tgctagcaga caagttttac atcctataca cttttcttca tttacagaca
    24961 taatattgtt ttctttaact atagcttttt gtggacatga gtttaaacaa ggagcatctt
    25021 cacactgtct acactgtatt ggcatacaga aattgtcacc ttttactaag taaagtctag
    25081 gtataacagg tatatcaact gttccaactg tataacccac attattatgc tgattatgca
    25141 ttgtaaaaca agctacttca caagctctac aacctgtaca tttgtttggg tctgctatta
    25201 caaaagagcc tattttattt ttcatattat cccacctttc aatttgtata tttccaaccc
    25261 tctagttatt ttcatcatat gtctacttac tttcatcata tatatttata tatttcacta
    25321 ttttctcagt acttatattt cgtttacatt ttttgcacaa attgtatgtg ttattataca
    25381 ccttatcagt atcatcaatt aatactccac agatttcaca ctttgaatat ctttgctctt
    25441 cttttttagt acacatatat tcattctcac tttttgataa ctctctgtgg atttgcataa
    25501 atattcatcc gtcttcctct tacaaatcca ataagcgtta tatttaattt tttagaaagc
    25561 tctattgata aattagtcgg tgctgatttt gatactacaa taggaatttg agtcatagct
    25621 gcttttaata tcatttccaa tgatattctt ccacttacag ccacaatttt atcttttaat
    25681 gttatgtcat ttaaaacaca aaatccaata gccttatcca ttgcattatg ccttgctaca
    25741 tcttcacaag tgactatttt tttatcaaaa ttaaatacag atacgctatg taccccacca
    25801 gtatttttaa ataattctga tgaatttaaa tttctatcca ttatactgta aatcgtatca
    25861 taatttattt ttatattgct ctttacagga gcacatgtta tatagtctag tgaatttaaa
    25921 aatataattt gctcatcttg atttttttgt ttctcttgaa tagttacttt tgcaaaacgt
    25981 tcactctcat ttatctcaaa ttttaataag ctttttttgt tttctatgta tccttttgtt
    26041 ttcaagaatc ctactataag ttcctctaaa ttatatgggg tacataaaaa tgtatttata
    26101 tatttatcat ttaaaataaa actaagtgga tattctgcaa ttatttcttc attatctatt
    26161 gatacttcat gttcatttat tttgcttact tctacattaa aagaatttaa atatttgtag
    26221 tccactagac tataactatt ttcaaaaact ttatccttac tattttcttg taatttagtt
    26281 tttgagtata tattgttcat aaaatattag cctttctgcc ttttataaat cttaaatcta
    26341 ataaatcatt agatatttgt agctgtcatt ttgtctttca aactttgata ctcttctctt
    26401 acacattttt ctgctaattc ttggtcttca agtttttcaa gtttaacagc acaatattta
    26461 tactctggtg ttttagatat aggatctaaa tttgctattg taagttcatt gcaagctcca
    26521 acccaccact gataagtcat atatgttgca ccttccttaa ctctatcagt aactgttgct
    26581 cttgttatta cacttcctct tcttgaacta attctaacta attcatcatc ttcaattcct
    26641 aatttttcag catcattaga atttatttga acacgtcctg gttcatcttc taatgaacta
    26701 agtgttctac aattaccagt cattgttctt actgaatagt gtccaacttc tcttacagta
    26761 cataaactaa atgggtattc atcatcttca acttccatag ggggtctcca ttcagcagcg
    26821 aacaaattac ctttaccatt aggagtagaa aacttttgac cttcatacag atacatagtt
    26881 cctttatctt ccatactttc actcttacaa ggccattgaa cgcatccttg agtttctatc
    26941 ttttcataag tagcacctaa gaaacttgga cataattgtc taagttcgtc ccatatttcc
    27001 ttagtatttt tataattcat aggatatccc attgctgtag aaatttcgct tataatttgc
    27061 cagtctggtt ttacatctcc ttgtggctct atagcttttc tcatcaattg gaatcctcta
    27121 tctgcacatg tatatactcc ctcatgttct ccccaagaag tagctggaag tataacatca
    27181 gcgtgaagtg ctgtcttatt cataaatata tcttgaacaa tgacaaattc caattcatct
    27241 aatgcttctc taacttctga tgcatctggg tcactttgca ctgggtcttc tccaaatata
    27301 taataagctt taagcttttt ctcttttagt actaaatttg gaacttgagt taaactataa
    27361 ccattattag gagataattt taccccccaa gctttttcaa acttttctct tatcttatca
    27421 tcagttacat tttgatagcc aggatataca ttaggaagag ctcccatatc acaagctcct
    27481 tgaacattat tttgtccacg tacaggtcct attccaacgc tttctctacc aaaattacct
    27541 gtaagtagag caattgatgc aagtccctta actacatcaa cagcttgacc aaattgacag
    27601 acacccattc cataaagtat catagctttt ttaccttttg catattctct catagctttt
    27661 cttataagct cttctgggat tcctgtaatc ttttcagcat atttagctgt atatggttta
    27721 acaatttctt tatactcatc aaatccttgt gtatgatttt gaacaaattc tttattgtaa
    27781 agaccttctt ctattaatac atttccaaaa gcatttacta aaaccatgtt tgttccacct
    27841 tttataggta gatgtatatc agcaattctt gcagattcag ttactctagg gtcagtaaca
    27901 attaatttag caccattttt tttagctttt actattctat ttgccactat tggatgtgaa
    27961 tctgctccat tatatccaaa tataaataaa acatctgcat tttctatctc tggtatagaa
    28021 ttagacattg caccactacc taatgagtaa gccagaccgg ctacagatgg tgcatgtcag
    28081 acacgagcac aatggtcaac attattagtt cctattgtag ctctcataaa tttttgcatt
    28141 atatagtttg cttcattacc aggtcctctt gcagaacctg ttcccataat agaatcagga
    28201 ccatattttt ctttaatttc atttagtctt gaagctgtat agtttatagc ctcatcccat
    28261 tcaacctctt ctagtacacc ttgttttctt atcattggct tttttagcct aggtgttaga
    28321 attttagggt catttagaaa atcccatcca taacgacctt ttaaacacaa actttcttga
    28381 tttgttctac cattagccgg ttcagctctt aatatcttat tatcttttac tactagatat
    28441 aattggcatc cagctccaca gtatggacaa actgttaaaa tttttttctc catacaattc
    28501 cccccttaga tttatcccct gataagtaaa atttatctta aagataatga gtgtttttat
    28561 aatatgcaaa atcataaata ttttgcaata ctatctatta aattcaacaa ttttctcaca
    28621 attcctctta aatttcaaaa aaaatatgtc ataataatct ttatatttca accatttaat
    28681 taaaatattc tagttcttat gacatatttt ttaatatact caatatatat tctttaaact
    28741 tatttagttt tcacttaaaa caatatttct gccaattcta tttttcattc tatagtgagt
    28801 aattacagta aatattccac ttccaatcag aatcaaatag tacataaatc caccatataa
    28861 aaatactgca tatcccatca aaggttgagg gaatactgat ttgaatatag tgaaaaaggc
    28921 taattcatta gctcctacat tcccaggtgt tggtattgga gatattgcca tatataaaaa
    28981 tgcttgtaat gtcagtatat aaatatagct ataaccttgt aaattaaatg ctttatatat
    29041 ccaaaatgaa acactaaagt aagcacttaa ttgaataaaa gttactatta tagttgaaat
    29101 taataccttt ttattctttg caaaaaaatt tattgcttta ctataatcat ctataaaact
    29161 ttctatggat tcaatcttat tatctaaaaa ttttaaaaac ttaatttttg ataaatgctt
    29221 tatagtaatt ctcataaaat attttatttt tttaggattt aaaataacta atattatcat
    29281 aattaagaca aatgaattaa tggctatccc tagaaacact agaggcataa tcattttcat
    29341 ttgtttttct aacatagcaa aattcattaa aacaagtatt gtacaataaa aagtaactac
    29401 tatttggaag ataatagact tattggtaac aactgcactt gccttactaa gtggcatttt
    29461 acatttttta agtacatata tctgtactgg ttggcttcct gaagcaaaag gagttattaa
    29521 attataataa aatcccataa tggctagctt aaaaccaata aatctaatat taactttgtg
    29581 agtgctttct ataattatct tcattactac tccttcaagc attatgtgac acattattgc
    29641 caaagcacct atgaataaaa acttcttatc taccataacc ataacatttg atagcatttt
    29701 aatatccaat gttttaaaca ctaggtaaat tgttatacct attaacaaaa ataaaaatgt
    29761 atactgtagt aaactctttc taatattctt tagtctatcc attagaaata accatccttt
    29821 gctcaagatt tatactaaat actatattat tagtatacac tatgaatctt aatattttat
    29881 aacaaaatac cttacaattt tgtcatttac aaaaaatatt aatctataaa ttaaatttta
    29941 atatatagat taatatttta caaacatttt gtagatatat ataatatttt cactaaacaa
    30001 cacttatact tattacaccc atttataccc tatcccccat acagtttcta ttgtataaat
    30061 atcatattgt tttaatttgc ttcgtatatt ttttatatgc tctgtgatag tacttatatc
    30121 actttctcca tcaaaaccat ataccgactc atatattctt tcttttgaaa atacttgtcc
    30181 cttatttttt gctagaaatt cacatattag atattcactt ttagtaaaat taattttttt
    30241 accatcaatt gaaacttctt tgcctaaaaa gttaaattga acatttgaga tagttaataa
    30301 attctttttt tctctacttt ctcgccttaa atgagcagtt atcctagccc ttaattctcc
    30361 tacaccaaaa ggtttggtta tatagtcatc tgcacctata ccaagaccgt acataatatc
    30421 actttccata ttttttgctg tcaaaaacag tattggacaa tcaaccttat ttcttatagt
    30481 tttacatact tcaaaaccat ccatcttagg catcataaca tctaatatta ttagattaaa
    30541 ataattaaaa tccatgttca tagctttttc tacatcacta attaccttta cttcatgtcc
    30601 atctcttttc aacacatttt caatcaactt taacatatct aactcatcat ctactactaa
    30661 tatcttagac ataataactc ctccatttat ccgtttaata gtattctaat ttatatattt
    30721 aataatatct ttaaaattca cattaaccat aatactctca tattatagct tacaataatt
    30781 aattactatt ttacaaataa aatattttag atacaaaaac tttatatttc aaaaacttta
    30841 tattcatttt acacgtattt acttaaatat aaaa
//
LOCUS       DAEMGG010000040        30305 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEMGG010000040 DAEMGG010000000
VERSION     DAEMGG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.040"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            177..866
                     /locus_tag="KPK24_001674"
     CDS             177..866
                     /locus_tag="KPK24_001674"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG2299351.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            867..1439
                     /gene="yqeK"
                     /locus_tag="KPK24_001675"
     CDS             867..1439
                     /gene="yqeK"
                     /locus_tag="KPK24_001675"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG2299352.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            1556..1906
                     /gene="rsfS"
                     /locus_tag="KPK24_001676"
     CDS             1556..1906
                     /gene="rsfS"
                     /locus_tag="KPK24_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG2299353.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            2260..4680
                     /locus_tag="KPK24_001677"
     CDS             2260..4680
                     /locus_tag="KPK24_001677"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG2299354.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            5516..6184
                     /locus_tag="KPK24_001678"
     CDS             5516..6184
                     /locus_tag="KPK24_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2299355.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(6502..6774)
                     /locus_tag="KPK24_001679"
     CDS             complement(6502..6774)
                     /locus_tag="KPK24_001679"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2299356.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(7005..7426)
                     /locus_tag="KPK24_001680"
                     /pseudo
     CDS             complement(7005..7426)
                     /locus_tag="KPK24_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7445..7543)
                     /locus_tag="KPK24_001681"
                     /pseudo
     CDS             complement(7445..7543)
                     /locus_tag="KPK24_001681"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            7826..9196
                     /locus_tag="KPK24_001682"
     CDS             7826..9196
                     /locus_tag="KPK24_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG2299357.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            9555..9713
                     /locus_tag="KPK24_001683"
     CDS             9555..9713
                     /locus_tag="KPK24_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299358.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     repeat_region   9999..10356
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9999..10027
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     regulatory      complement(10498..10595)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            10821..11204
                     /locus_tag="KPK24_001684"
                     /pseudo
     CDS             10821..11204
                     /locus_tag="KPK24_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(11393..12388)
                     /locus_tag="KPK24_001685"
     CDS             complement(11393..12388)
                     /locus_tag="KPK24_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG2299359.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(12509..14137)
                     /gene="aspD"
                     /locus_tag="KPK24_001686"
     CDS             complement(12509..14137)
                     /gene="aspD"
                     /locus_tag="KPK24_001686"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG2299360.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            14796..16013
                     /locus_tag="KPK24_001687"
     CDS             14796..16013
                     /locus_tag="KPK24_001687"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG2299361.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            16108..16488
                     /locus_tag="KPK24_001688"
     CDS             16108..16488
                     /locus_tag="KPK24_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG2299362.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            16772..17254
                     /locus_tag="KPK24_001689"
     CDS             16772..17254
                     /locus_tag="KPK24_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2299363.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            17275..18090
                     /locus_tag="KPK24_001690"
     CDS             17275..18090
                     /locus_tag="KPK24_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2299364.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            18143..19693
                     /locus_tag="KPK24_001691"
     CDS             18143..19693
                     /locus_tag="KPK24_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2299365.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGYIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSVIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            19790..21142
                     /locus_tag="KPK24_001692"
     CDS             19790..21142
                     /locus_tag="KPK24_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2299366.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            21273..22031
                     /locus_tag="KPK24_001693"
     CDS             21273..22031
                     /locus_tag="KPK24_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2299367.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLISNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELFNLLHTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            22078..22797
                     /locus_tag="KPK24_001694"
     CDS             22078..22797
                     /locus_tag="KPK24_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG2299368.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(22921..24321)
                     /locus_tag="KPK24_001695"
     CDS             complement(22921..24321)
                     /locus_tag="KPK24_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG2299369.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(24325..25233)
                     /locus_tag="KPK24_001696"
     CDS             complement(24325..25233)
                     /locus_tag="KPK24_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2299370.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPASEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKMIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            25494..26141
                     /locus_tag="KPK24_001697"
     CDS             25494..26141
                     /locus_tag="KPK24_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2299371.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            26465..29104
                     /locus_tag="KPK24_001698"
     CDS             26465..29104
                     /locus_tag="KPK24_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG2299372.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(29162..30265)
                     /locus_tag="KPK24_001699"
     CDS             complement(29162..30265)
                     /locus_tag="KPK24_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG2299373.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIHLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
BASE COUNT        11397 a         3718 c         5067 g        10123 t
ORIGIN      
        1 aaaatagtaa aaagttattt ttattaaaaa gtaaagtaga atcatgttaa ataattattt
       61 ttttaagtgt tttttagcat ataaatataa ttatataccc aaaaaaacaa attataagct
      121 ttaagaaata aaaaataatg gtacaatagt attataacat taaaaggaga caaaatatga
      181 gaagtgaaaa tctagttaag atggcagagt tgaaaaatac tgaaaaacta gagaaattta
      241 atcgccataa aggcaaaatc aaaataggta tattgggagg tacttttgac cctatacact
      301 atgctcattt agcaacagca gaatttatta gagacaagta tgacattgac aaaataatct
      361 ttataccatc aggaaatcca ccacataaat tatgtatcac tacagataag tatgatagat
      421 ataatatgac acttctagca actgaaagca acgaagattt tttagtttca aaagtagaaa
      481 tcgaaagaaa gaaaaggact tatacaatag atacattaaa atacttaaag aaaaaataca
      541 aaaatgcaga catttatttt ataacaggtg cagatgcaat ttgtagtgtt gaagagtgga
      601 aagatgtaaa gaaaaacttt gagttagcta cttttatagc cgcaactaga ccaggaatta
      661 gtttgttaag gtcacaagag actatagaaa aactaactaa gaagtataat gctgacatta
      721 taacagttta tgttccatca ctggatatat cgtccacata tataagggaa cagctaaatg
      781 aaggcaagtc aatacgttac ttagtacccg aaaatgtaga aaattattta tatgaaaata
      841 aactatatca atatggagat gattaaatga acttagaaaa tattaaccaa agactaaatc
      901 aaatgctacc agtggggagg cttagccatt caaaaaatgt tgcaaaatgt gctgaaaaat
      961 tatgtgagat ttatggatgt gacaaagaaa aagcttactt agcaggaatg attcatgatt
     1021 gtgcaaagta tttaagtgac aaggaaatag aggattatgt aaataagtat gaaatatatt
     1081 tagacccaat agaggatgga aatcgttcct tgtctcatag cgtaattggg gcgtacatat
     1141 gcgagtatga atttgaagta gaagatgaag atattataaa tgcaatcaaa tatcatacaa
     1201 cagggagaga ggacatgtct cttttagaaa aaataattta catagctgat ttaattgaag
     1261 aaggaagaaa attcccagtt gtagatacat taagagaatt agcatatgga gggaagcttg
     1321 atgaagcact tttaacttct tttaataata ctttaatgtt tgtcatcaat aaaaaagaag
     1381 agatacatcc aagaacagtt atggcaagaa actatctaat taaagaaaag ttattataaa
     1441 aaagctatat aaaggcaaac atattaataa atttaaattt gactatgtta caaaatcgta
     1501 ttaatatgtt acaataacta agtgtatttt aattaggaaa gtgaggaatt tttatatgac
     1561 agtggaacaa atgacaaaaa tagcatatga tgccatagaa gataaactag gtcaagatac
     1621 agttataata aatataggaa aagtttctag tttgtgtgat tattttatta ttactacagc
     1681 atcttctcag agacaggtaa aagctattgc tgacaatgtg gaagatgaat tagcaaaact
     1741 aggattagag ccaagaggta aagaaggaca aggaactcaa acttgggtac tccttgatta
     1801 tggagacata atggttcatg tatttaatga ggaaaataga gggttttata atttagaaaa
     1861 attatggaaa gatgcacctt atatagatat tgacacatta gcataaacat gataatatat
     1921 gttattataa ttagaaaatt tcaaaaatta tattaaattg aatagactag agcagtaaaa
     1981 gattatattt tttcagagag ttagtggaag gtgagaacta atattatata actttgaact
     2041 tactagatta aaatctattc tccatggctg gattaaaagc tatctaagag agtctatata
     2101 tgtagtaggc taattagggt ggtaccgcga agatttatcc tcgtccctaa acgtaagttt
     2161 agtgacgagg attttttatt ttcaaagaat tatttggaaa ttataagtaa ataatactaa
     2221 ataaggtagt tatacacaat taaaaatagg aggcaaaata tgagcgtata taattttaaa
     2281 gaagtagaat cgaaatggca aaaaatctgg aaagataata atcagtataa aatggatact
     2341 gcacaaactg aaaaacctaa ttattacaca ttagaaatgt tcccttatcc atctggaaaa
     2401 atacacatgg gacatgttag aaattattct ataggtgatg ttgtcgcaag attcaaaaaa
     2461 atggaaggat ataatgtact tcatccaatg ggatgggatt cttttggatt accagctgaa
     2521 aatgcagcta taaaacatgg aatacatccg cataaatgga caatggaaaa tatagaagaa
     2581 atgaaagagc aattaaattt attagggatt agttatgatt gggataaaga agttgcaact
     2641 tcaactccag aatactatag atttacacaa gaaatatttt tgaagttttt agagcatggg
     2701 cttgcatata agaaaaaatc atatgttaac tggtgtcctt cttgtgagac tgttcttgca
     2761 aatgagcaag ttgttcaagg agcatgtgag agatgtaaag caacagtatt aaagaaagat
     2821 ttagaacaat ggtattttaa gactactgag tttgcagaag aattacttaa tgacttagat
     2881 acattagatg gatggccaga aaaagttaaa acaatgcaaa aaaattggat aggtaaaagt
     2941 acaggagcag atttagtttt tgatatagat ggaactgata aatctatgac agtatttaca
     3001 actagaccag acacaactta tggagttact tatatggtac ttgctccaga gcatgaatta
     3061 gttaaagaat tggttgcagg gactgaatat gaagcagatg ttgaagcttt tgttcaaaaa
     3121 atgcatacta tgacagaaat agaaagaaca gcagctgatg tagaaaaaga aggaatgttt
     3181 ataggtagat atgttataaa ccctttaaat ggcaagaaag ttcctctatg gatagctaac
     3241 tatgtattag ttgagtatgg aacgggtgct ataatggcag ttccagcaca tgatgaaaga
     3301 gatagagatt ttgctgaaaa atataattta gacataattg atgtaataac agaagataac
     3361 aaaatgataa attcagaaga atttaatgga ttagatgctt cagaaggatt tgaaggaata
     3421 atcgataagt tagaaaaaga aggtagagga aagagaacaa ttaattatag acttagagac
     3481 tggttagttt caagacaaag atactggggt tgtcctatac ctgtggttta ttgtgatgaa
     3541 tgtggaatag ttccagttaa aaaggaagat ttaccagttc ttttaccaac agatgttgaa
     3601 tttactggaa aaggggaatc tccacttacg acttcaaagc aatttatgtc aacaacttgt
     3661 cctcactgtg gaaaaccagc tagaagggaa gttgatacta tggatacatt tgttgattct
     3721 tcttggtatt tcttaagata tgtggatagc aataatgaaa atgaaccatt tagtaaggaa
     3781 ttagttaata gatggcatcc agtagaccaa tatataggtg gagtagaaca tgctataatg
     3841 catttacttt atgcaagatg gtttgtaaag gcatttaaga gtatgggaat ggtagacttc
     3901 aatgagccat ttaaaaactt acttactcaa ggaatggtac ttatggatgg ttctaaaatg
     3961 agtaagtcta aagggaatac agtatctcct atggatataa tagatgagta tggagcagat
     4021 actgcaagac ttttcgtatt atttgcagca ccaccagaaa gagatttgga ctggtcagag
     4081 caaggtgttg atggatgctt tagattctta aatagggttt atagattggt tgacgagtta
     4141 gcagatgtgg ttaaaaagga tgtagagttt ggagaattaa attcccaaga taaggatatg
     4201 agatatacta tacactctac acttaaaaaa gttactgctg atttaagtga aaaattcgga
     4261 tttaatactg ctatatcagc tcttatggaa ttaataaatg atatgtataa atataaagag
     4321 ttagataata taaacgaagc agttataaaa gaaggtgtac aaactatagt aactataatt
     4381 gcaccatttg cccctcattt aggtgaagaa ctatggacta tgataggtaa agaaggaagt
     4441 gtatttgata tagactggcc taaatatgac gaaaaagctt tagtaaaaga tgaaatagaa
     4501 gttgttgtac aagttaatgg aaaagttaga ggaaaattaa ctgttaactc aaatatatct
     4561 aaagatgaga tggaaaaggt agctttagaa gatgaaaaaa taaaaggatt agttgaaggt
     4621 aaaactatag ttaaagttgt agctgtacct aaaaaattag taaatatagt tgttaaataa
     4681 ctcaaaaata tgaacaaaaa tataaattta taaaataaat gaggctgtct caaaatagag
     4741 ataagccttt tttcattgtt gaaattataa aaatagattt gattttatac aaaaaaatga
     4801 ttaaagaact aaaaagttca ttcttatata ccctagttgt tgttttaaat attattataa
     4861 ttaatgttaa atcgagttaa tgtattaagt ggtatgtaaa tcattcagtt tccaaagtgt
     4921 tgttcgtcaa ggaactgctc taagcttaaa cgaaaaaaat ataattattg tattttgaaa
     4981 tttttttcta tataatgaaa acaggcaaaa aaggtgcata tctatgcttt atatgaaaca
     5041 cccagacaac gaaattcaga ttcatataat agctaccatg tttctaaaaa ggaacttgta
     5101 aaaaagatag cgtttcaact tcccagttga ttaaaattat cagcaggaaa acaagtatgg
     5161 tcagcagtgg tattctaatg ggtgttgtcg atatatccaa gttgggattg tctgaggatg
     5221 ttatcaaata acttgtgata ggtgcagtag atgtggagat tcctgatacg gatttattct
     5281 caggggatgg ttgcttcggg agatatggag cgtaaccagt ttattgatat ggtaaccatt
     5341 ttttgtctaa tttgatgaaa gaatcccaag aataccatac agaagtaaga caggaattaa
     5401 ctggtggtat aaactgataa aggcaaattt aaaaattttg ataggatgat tttaagattt
     5461 atcttctatt ttgtttttta gaaataattg aaatgttatg aggaggtatg tacaaatgga
     5521 gactgtaaca aataatcaat caggtgtaga aattcaactc agtctgtttg aagctatcaa
     5581 agatttattg ttaattaatc ctattttctt gttgggaatt atcttgattg gtattttctt
     5641 agttgtaata ttcaagaaaa atagtgatat tccaaaggtc aaaacatcta ttttatcact
     5701 ggcaatgtat tactacttat gtttaacact atcgcatatt gttggtatac ctacattcgg
     5761 tgaatatata agactgtcac agttagggga aacgttcttt aatccaaaca taaatcttat
     5821 tccttttagt gatggattta gtttgagttt tattttgaat attttacttt ttgtcccatt
     5881 aggtttttta tgtccgctta taagcaaaac ctttgaacgt ttgagaaata cattcttgat
     5941 tggttttgga ttatctcttt ttatcgaaac tattcagctt tttacgttac acagagcaac
     6001 cgatatagac gacttactca cgaacgtaat tggaacaata ataggctatt tctgcttcaa
     6061 actgattgct aaattgcgaa ttgtaaaact acattcagat gatgactcta tggaacagga
     6121 ttacacagta tgtattccga ttgccattat aatagttgcc cttatattag gattttttag
     6181 ttaagttcca aatagctaat ttgggaatcc agacaatata acagatacta tgttaccttt
     6241 tacaggagca tactatctgt tatattgtca acatcctatt ttatggtttg tgtgtagttt
     6301 cctgtaaact gaactaaata attaaataga agctattaga attaataata acagatagat
     6361 attaataagc ataaacaaat agtaaaatgt gtagatatag acgtttttaa gtgaaactat
     6421 tataaaataa ttgactgata atcaactgac aaaacctatt tttttaggtt taactgacat
     6481 ttaactgaca gtcagttttt actatttaaa tagaagttcg cttagattgt cggaagcttc
     6541 tttgtccatt tctttaagta catgagaata tctattcata gttattttta tatctgtatg
     6601 acccaatctt tcagaaacaa ctttcatatt agttccccct aaaagcagca gagtggcact
     6661 tgtatatctt aaatcatgta ttctaatctt ttttaaatta ttttttttgt aaaattgtga
     6721 aacgtttttc ttagtgaaga aggtagaaac ggatttaaat tggtgtttag acaaactaag
     6781 ttatttctgt tttccaggat accttacaat tttaatttgc tttgtcttag tttttcaatt
     6841 ttcaattttt caattaattc ttttggagct gataaagctc tttttgattt tggtgttttt
     6901 ggctctttaa aagatatttt gccattagaa tagacaagaa tttgattaac atttatcaga
     6961 ttgttatcta agtcaacatc tgaccaccta agcccaatag cttctcacat tcttaaacct
     7021 agaagtaaca ttaaaagaat aggtatttct atattattac caagtaactt ttctattagc
     7081 aataatactt cttctctatt gtatatttca tttgtagaat atttttcttt agaaggaagt
     7141 tttataaaat cacatgaatt ttcttgtatt tctcttaatc tatacgcttc ccttattaca
     7201 gaacttagaa agctatacct tactttagca ctagtaaagg tagaattatt aaagttataa
     7261 tttacaaaag cttgtactga actaggattt acttctgtga gttttagatt cttaaaaaag
     7321 gttctatgta atttttaacc caagattttg tatttgtaac agtatatgga gaccaattat
     7381 ttttattcat gtctatatat ttataacatc tttctacaaa tgtcatacct tttggagcaa
     7441 caaacttatt attatttata gtagacttta tttcaattaa atgtttttca gcatcctttt
     7501 tctttttata gcttccataa cttttttgtt tctttttgcc agtttcttat tccacatata
     7561 catagaattt ttcccatctt tttcttataa aagctgattt gatattcata tatgtacgct
     7621 ccctttataa gttagtttta taaaagaaca gttggactgc tctaattatg tcattatctt
     7681 tttaattctt tacagttaca ttataacacg tatttgtata cacttaaatt aacaggaaga
     7741 taaatatcgt taaaggagga aaagaaaatt taatgtagaa ttatgtaaga tatatagtat
     7801 ttcatatata aaagggggag atattgtgaa attaaagaaa ttattatcac tagggttagc
     7861 cgtgtcaatg tttgctgctg gtcctatatc agctaatgca cttgatacaa tttatactat
     7921 tcaaggaaaa gataaatatg aaacggctgc tcttatagct gatgaacaaa attatactac
     7981 agctatatta attaatgcag acagcacaat ggctgatgga ttaagtgcga gtggacttgc
     8041 aggagcaaca aatgcaccaa tttttttaac taagaaaaat aacataccta atgcaactct
     8101 taaaagagta gagaaagcta aaaaggtata cataataggt ggagaaagtt ctattgataa
     8161 agcaacagaa actcttctaa aagataaagg aatagaggct aaaagacttc aaggtagtga
     8221 tagaattaag acaagctata atgttgcaaa agaaataaac tcaattaaca aagtaaataa
     8281 agttatattg actaatgctt ttaaaggaga gcctgatgca atgagtgctg cacctgtagc
     8341 agttagagat aaagcagcta tagtattaac tgatggaaaa agtgttccat ttaatacaac
     8401 tggtgtagaa agttatgtaa taggtggtac ttcatcaatg agtgacaaac tagttaatga
     8461 tactaattca actagattag gtggagtaga tagatatgat actaataaaa aaatagtaaa
     8521 taagttctat aatggcgcaa aagaatttta tatagctagt ggtactgatt tagtttatgc
     8581 tttagttgct tcaccaatgg ctaaaaataa tcctattgta ctagttgata ttggaagtaa
     8641 taaagatata cttaaaaatg ctgcaaaagt gactgggata ggaaatctaa gtgataaaat
     8701 tatgctagag tgtgaaaata ccatcaataa tattagtctt ccgtcttcaa ataaaaatat
     8761 aatatgtgga ttaaagattg attttgattc aaagcacata atttggcaag ataaaggaag
     8821 ttatcttgaa tgttatgatg ataaccttga atattatact gaaaaatata aagacccaac
     8881 agttcctata aatatagaaa atacaagtac acaaaattta gatgtaacaa aattaaaagt
     8941 ttatggagaa gctaattcaa cattatatat ggggcaatat gctaaaataa aaatggattt
     9001 taatagtttc ggtgtattaa agccaggaga aaaatgtact atttatttag atataatttc
     9061 attagatcca tcaagaagca tgacattaac attttcttat gtaggtgata aagaaaatta
     9121 taaatatgaa caaggattgg caagtagaat attattttat aacccagatg gagaagattt
     9181 ttttgaatat aaataactag tgggtatgta aattaataag ggttttataa gtttaatttt
     9241 tttattttcg ttttatatta actatgtggt atgtaaatgt ctcgatattg ctcatatgtt
     9301 gagttattat atttagtttc atatgaacta tatggatttt aaaattaaaa atacttaaaa
     9361 acatttactt taatagtagg tgtttttttg attaaaagaa tattattata atgtaaaaag
     9421 taataaatag gtaaaatatg taagaattat atgttataat atttttagca agaagatgta
     9481 atctacaatt tatagagtgg agttcatgta tcaaaaaatt atcctcccga cgctaagaag
     9541 ggaggtgaat atacatggat aattttttac aaggcatact agcaagtcta tctgctagct
     9601 taatagttta tatagctagc aaactattta gaaagcgtaa aaaaccactc aaagcggcaa
     9661 ctaagagtgg ttgggaattt gatttaaaaa tcagattcca taaaactaag taatttctaa
     9721 attatgaact ccactctaca gcaaaataga ttgtagttct tcttgctttt attatatcac
     9781 aaattggtac agatattcaa aaataatatt tttatgatat aataaagtca tagaaatttt
     9841 gcagtgttcg attttttgaa gaaattaagg cttaacagtt gaaatataag gcattgagga
     9901 cttgtgataa gtgttatcaa ttgcactact catggttcac tgcaaatttg agagatttgt
     9961 atatgtgtag gcattggaaa tactcaattt attttggggt tttatattaa ctatatggaa
    10021 tgtaaatatt ttttttatat ataaacttct aaacttttac aaaagtttta tattaactat
    10081 atggaatgta aatacacttc caacaagcat tgtagggttg aagtctacag gttttatatt
    10141 aactatatgg aatgtaaatt aaatcgtatt tataattata tctacactta gtaaatgttt
    10201 tatattaact atatggaatg taaatccaat agaccctcca ataactccag atattccttc
    10261 tggttttata ttaactatat ggaatgtaaa taatgaacta tctaaaccaa ggtcataatt
    10321 taattgagtt ttatattaac tatatggaat gtaaattgta tccaaaaaat tacaataatc
    10381 atacgaattg ctaatataag cagtcaatat atgtgtacat ttatccattt tatctctaaa
    10441 catataaatt attatccccc taaaaaactt tcataaaaac aaaaagaaca atatctcttt
    10501 ggcaactggc taacataact cataagatat ttatataagt cttagtccct atagctttgc
    10561 gtcactagat ttctctagtt ttgccgattt agttttattc tataactaaa taatacaata
    10621 gaattagtta ttattcaaca agattgtttg aaaattgaat aaatttatag gtatgaacgt
    10681 aatttcacac ttaaaaacag actaaatacg tagaatatat agaaaaattt acctgtagtt
    10741 tttgtgataa aaagctccta ttattaattt aagctagata agtttgagga agtgacataa
    10801 attgaaagaa ggaatatatt atgttgaata aaaaattacc agatgcagaa ttaaaaatta
    10861 tgaaatacat atggaatact ggttataaaa ctgtaatatc aaaagatgtt gcagattaaa
    10921 tagaaaaaac atatgcttgg aagcatacaa ctacaattac acttttagct agattaacta
    10981 aaaaaggttt tttcatatcg cagagaattg ggaaacatgt acactataga gtattaataa
    11041 aagagaaaga atatctcaaa atagagggaa aaagaatttt tggaggtcta cataacaatc
    11101 cattatcaga actaatttca aaattacatg atggagaaga aataactgaa gaaaaaataa
    11161 tagaaatagg aaattggata aaaagctgga aagatgaaga ttaataaata aaaggtagct
    11221 actaatataa gtgctacctt ttttcttgtg gataatattg gggataagtt gtggaaaatt
    11281 tgtgtaaaaa ttgtggataa aatattttgc tgacatttaa ctgacataag tattagattt
    11341 tgatttaaaa atgttgaaat atcaataaat aattaaatag aagttattag aattaacaat
    11401 agattccttt ttcaaagaag tctttgattt catcaaggtt atcagttttt ccaagtgcta
    11461 agataagttt tattctagct ttttgacctg gtaagtcccc accaaatata caaccaagat
    11521 tttttaaatc tctaccagaa ccaaagtaac cataactatc gaatactcta cctgaatgac
    11581 atctagaaac tataactaca ggaagtcctt tttctctagc atattcaact ccagaaagca
    11641 tttgaggagg aatattacct ctgcccattg cctcaataac tataccttta taaccactat
    11701 caacagcaaa tcttataaag tcagcatttt ctcctacata tgctttaaat aaggcaactt
    11761 tagattctac cttatcagta tctataattg ttctatttac aatatctctt gtaaggacaa
    11821 gttcgttgca gtcaattatt cctaatgggc cactagttag agatttaaat gtatttagag
    11881 aaagagtatt tgttttagta gcttcagaag ctaataatac ttcattgttt aaaactacaa
    11941 ggactccttt tcccatagca tctttggata ctgcagtaca aactgatgca gataaattac
    12001 ttgaaccatc atatccaagt tcagaactac ttctcatagc acctgtgact attacaggtt
    12061 tgatgttgtc aattgtaagg tctagaaaat aagctgtttc ctctaaacta tctgtaccat
    12121 gtgttattac aacacctgta atatcttttc ttgctaataa atcatttaca taatttttca
    12181 attccatcat tctataaggt gttatgtgag gtcctggaat ttcatcaaaa ttaaaaactt
    12241 caacatctgc aactttatca atgttggtaa ccattgatag tatttgttca cctgataaag
    12301 ttggtatagc tgctccgact ttatcatcta ctgtcataga tatagttcca ccagtaaaaa
    12361 ctatggcaac ttttttctta atactcatac tattctctgc ctttcataaa taaaatttat
    12421 aattccgata ggattatatc aaaaaaagta gttaaggtct acataaactc ttttattcat
    12481 ataatttaga tatttcagtg taatataatt atttcttaat tttttgccat tcaacgatat
    12541 aaccatttaa tatctctctt atggcttttc ctatcttagt ataagcttca tcatatagat
    12601 ttgctagaga tattctaatt gaccattcag ggccatagaa tccatttcca ctaagtaaaa
    12661 ctactgaata gtcattagct aatttataaa gaacatctac tggagatgca gttttactta
    12721 taaatttaaa taagtcttct ccgtagttta atttagccca ttcttcaata tcaaattgag
    12781 tgtaatatgc agcattgttt ttgttttcat taattggaag ctctaaagct tcaaataata
    12841 gtttttgacg tctatggcag atactcatat taagtttttt gtaggcatct actttgtcaa
    12901 tcaatgcaaa agcagagaaa aacgccattt gtacttgctg tggagtagaa agaccagcag
    12961 tatggttgag agcaacaagt ctgctatcag caacaactct ttccataaag gaaagactag
    13021 aagggttaag actcatatca ctgtaacgct tatctactga tttttttaat tctccagtta
    13081 aatcatttat tttcttatca aaaacatttt tcttatgaag ggcaaatgta ccaagtctcc
    13141 atcctgtaac tccaaaatat tttgaataag aatatactcc aacagtgttg taaggtagtt
    13201 ttgacattaa ggatgaaaaa tcttctacaa aagttccata tacatcatct gatattatca
    13261 ttaaatcttt attataattt tcaattacat ctattagatt attacaagat gtttcatcca
    13321 tagctataga agcaggatta ttaggattaa caacgaataa agctttaata gatttgtcac
    13381 gaagtttctc taattctttt tttgtatatt gccatgtatg agcacctttt tcatcaactt
    13441 cattagcatt tatatttacc actttaaagt cgtaacgagg aaggttaggt atttcaagat
    13501 aaggtgtaaa tattggagtc attaaggcta tggtatctcc tttttttaga agttcatttg
    13561 ccattaatga atcaaatata tagcacatag cagctgtagc accttcaaca gcaaaaattt
    13621 ctacatcacc atatttacct gatatatcat atttcatttc ttgaactagg taatcgtgta
    13681 ctattttttc catatgaggt aacattctat ctggaagtgg atagttatca ccaataattc
    13741 catcagctag ttcatgaaca aattcatctc catcaaatcc aagttcattt ataccaaagt
    13801 ttataatatc tctagctaaa tatgctccag gcatatctat attttcattt ataaatttaa
    13861 aaaatctttc tttaataccc tttttttgag gcattccagc caaatgacct aaatcccaat
    13921 tagaacgagt ttcagtaata gcgaaatgac caaaagtaaa aaaggcttct ctagcagttg
    13981 aacaagtcca gtttggattt ccacgacctg catcaagtaa ttctctgtta ttttcttttg
    14041 cagtgtattt tgcaatatct attaatttgt ccttaaattc aaaagggctt atttttccat
    14101 atatgttttt gatttcttta taagtatgat ttttcatgtt aaattcctcc ttatgtaata
    14161 agtttcacat ttttataata aaataaacaa gttgcaaaaa tgattatact ttaattataa
    14221 taggtttata gaaaaagtaa agtatgaaat taaaataata actctattta gccttattaa
    14281 atatatgtta aaataaaaaa taccttttaa taaaaaatga ttgttatata aaataaatat
    14341 tcaatttaat tatcacctta gtttttttat tgtattttat tatatatgta tagttttcta
    14401 attttgaata aaaataaagt aaaactttct tttatattga agttgtttag taagatatgt
    14461 attttattaa tttattaatc ttaataatgt taaataaaat caaaaaaata gattttgggt
    14521 aaataaaata atataaaata taaattttaa taaaaataat aaagtatata ttattgcttt
    14581 aataagaatg taaaaaatat ttttaaatta tcatttaatg taatttaaat gaattgaaga
    14641 tatatgttta ttgtatgcaa ttgttgtatt tttaggtttt ttgtgattaa ttaaaaaatt
    14701 ataaaaatcg tttacatgtt gatttttaaa aaaaaaagat atagaattat atagaaagta
    14761 ataaacaaaa attggattta cgggagtgga atgaaatgat gaatgttacc ttaacagatg
    14821 taaaagaggc tagagagacc attaagaaca tagtaaaaag aactgactta cttgaaagtg
    14881 ttaaacttag cgaaaaaaca ggagcaaatg tgttctataa atgtgagaat cttcaaaaaa
    14941 caggttcttt taaattaaga ggagcttgta acaaaatagc gagtttaaca gatgaagaaa
    15001 aggcaaatgg agttatagct tcaagtgcag ggaatcatgc tcaaggagtt gcattgggag
    15061 caaaaatgac tggtataaaa tctacaatag taatgccagc aactgcgcct ttagcaaaag
    15121 ttagtgcaac taaaggttat ggagcagaag tagttttaaa tggtgcggtt tatgatgatg
    15181 catatgcaaa agctgttgaa atacaaaaag aaacaggagc aactttctta catccattca
    15241 atgataagta tgttatagca ggacaaggaa ctatatctct tgaaattttc gaacaattag
    15301 acaataaagt tgatactata ctatgcccag ttggtggagg aggaatcatc tctggtgttg
    15361 ctgttgcagc aaaagcatta aatcctaatg taaaaatagt cggtgttcaa actgcaaata
    15421 taccttcaat gaaagagtca ataaaaaatg gaaaagtaac aacagcattt aatgatacta
    15481 caatagctga tggtatagct gttaaaactc caggagattt aacttttgaa ataataaatg
    15541 aattggttga tgaaatagta gtggttgaag agacagaaat tgcagaatca atattattta
    15601 tgatggaaag tcaaaaaata gtttcagaag gtgctggtgc tgtatgtaca gctgctatat
    15661 taagtggtaa gtatgtacca gcaaaagatg aaaatgtagt ttgtataata tctggtggta
    15721 atatagacat aaatacgtta tatagaataa taggtgttgc tttagctaaa gaaggaagaa
    15781 gatattcatt tagtacaatt atggaagata agccaggaaa ctttgctgag ttaactagaa
    15841 taataagtga gaatggtgga aacattttaa gtgctaatca aggtaaatta tcagcagggg
    15901 aagctctagg aaaacaaagt gcggaattta tattagagac aatagattat gaccatatag
    15961 ctagaataaa gaaagctata gaagaaaaag gttttaaaat tatagaatta taataaaaag
    16021 tgtatataat atacaagaaa aatagtataa tagtgtatga agatacattt tatgtgtatt
    16081 agattaacga attaggagga ttttaaaatg aaacatgaag taatacatac taatgatgca
    16141 ccagcagctt taggaccata ttctcaagct ataaaagctg gaaacttgtt atttgtatct
    16201 ggacaagttc ctcttgtacc agagactatg gaagtagttg aaggagatgt acaagctcaa
    16261 actgctcaat ctttaaaaaa tcttaaagct atattagctg aaagtggagc tgatttttca
    16321 aatgtagtta agactacagt atttataaaa gatatgaatg aatttggggc tataaacgaa
    16381 gtttatgctg aatattttgg agaaaataaa ccagcaagag cttgtgtaga agttgctaga
    16441 ttaccaaaag acgttaaagt tgaaatagaa gtaatagctg tattataaaa atacaatatt
    16501 tttaatttga aaaggctgaa aaactatatt ctagtgattc agccttttta taaaaaaata
    16561 aatcacaata aagaacatga taaatattgt cgaattaaaa aataaaatta aaataaatat
    16621 tgcaatattt ttgagaaggt tttataatga gtatataatt tgaaacgtgt aactattaaa
    16681 ttaggcatga gttcttggat atttttatcc tgaggattca tgcctttttt gtattgaaat
    16741 tcaaaaatct atttaataag gaggaagtac tgtgttaaat tttttcaaga aaaataaaag
    16801 ctataagtta catgcagtag tgagtggtaa cagcattaat attgagaaag taaacgattc
    16861 tgtgttttca aaaaagttaa tgggagatgg agttgcaatt attcctaata gtaatgttgt
    16921 tgtagcccct tgtaatggta aagtaactgt gcttacagaa tcaaagcatg cctttggtat
    16981 ggtctcagat gagggtgttg aaatattagt ccacatagga attgatacag ttagtttaca
    17041 gggtgagggg tttaaaaatg aagtgtctca aggagatact gttaaaaaag gtagtccaat
    17101 aataagtttt gaaagagaaa aaataaattc tcagggaatt gattgtacaa caataataat
    17161 tgtattaaat catagcgaat tttcagaaat aaattgcatg gtagaaaatg aagttgttgc
    17221 agggcaagat actgttattg aaattatgaa ataatagaaa gggattaatt caatatgaga
    17281 gtagtaaaaa tctttaataa taatgcatta tctacagtaa ctaatgatag gaaagaagct
    17341 attttactag gtcttggtat aggttttaat aaacgtccag gagataaagt taatgaagat
    17401 aaaatagaaa agatttatta tgttcaagac cacatgcaaa caaaattttt agaattatta
    17461 aaaaatgtta gtccagaagt gatggatgct tcacaacaaa ttataagtct tataccagga
    17521 aatgagggaa aatttaataa taaagggata ttatctttag tggaacatat tagttttgca
    17581 atagatagaa tgaaaaataa tgttttttta cctaatttaa tgcttttgga tataaagatg
    17641 atgtattcaa aagagtttga attaggagta aaagctttag aaattatata tagagtatgt
    17701 catatccatt tgcctgaaga tgaagcaggt tacatagctt tacattttgt caatttacaa
    17761 tcaaatgata atttggcata tgatacttta aaatttgtaa aaggtagtat tgatttgatt
    17821 aaagaatgtt atggattaga attagacgaa tcgagtttaa gtacattacg ttttagaact
    17881 catcttaaat ttcttgctca aagaattttt aagaatgaaa tttgtcagga tgataaaatg
    17941 attgaaatgt atgattattt aattaacaat catcctaaaa ataaagaata tttagaaaag
    18001 ttaaatgtat atattgaaaa ggagtttagg tataaattag ataaacctga aaaaatttat
    18061 ctactactac acctaacaaa aatcttataa gaatgagatg aattagtaat agaaatatat
    18121 ataattttag ggggggataa gattgaaaga aaaactactt ggaggactgg aaacatttgc
    18181 aaaggcaatg gttcagccat taatgtattt atcgagtgca ggaatattaa tggtggtagg
    18241 tgtactactt acaaatgata tgttattagg agtacttcca ttttttaaat gggagccaat
    18301 tcaaatattt ggaagcctaa tttataactg tataatggct attattaata atcttgctgt
    18361 tatatttgct ataggtattc ctgctgcact agcaaaaaaa gagaagcaca aagcagcgtt
    18421 aataggattt atgagttata taatatacct tacagcttcc aacactatgt taacatcact
    18481 aggtcaacta gcaaaagaaa gcaagatgct tggactgata gggactggac aagctactat
    18541 tttaggtatt caagttgtag atacaagtgt atttggtgga attattttag ggtatatagc
    18601 tggatatgta tttaatcgta caagcaacaa aacatttaaa ggagctttac agatatactc
    18661 gggagcaaat tttagtttta tatgcatgtt ctttgtatcc attttaattg gagttgtgag
    18721 taactttata tggccattag ctcaacaagg aatagcttct cttgcaaatg tcatcaaaga
    18781 tacaggtaca tttggtttat tcttatatgg ttttttagag agaatattag taccaactgg
    18841 acttcatcat ttaatttata caccatttca gttttctgat ttaggaggag tgcttaaagt
    18901 tggtgcagat acagtttcag gagcctatgc tatagttatg acagaaatga atttaccagt
    18961 tgagcgtttc tcagatagta tatactacat ggcaactgga tttacaaaga tgtttggtta
    19021 tataggtata ggagctgcct ttatttatac tgcatataag caaaataaga agaaggttaa
    19081 ggcaacgatt attccactaa taattacggc agtattagcc tctgtaacag aaccattaga
    19141 ctttatgttt gtattctgtg caccactttt attctttgta catgcagtta tagctggtct
    19201 ttttatagct ttattaaaaa tatttgatgt tactgcatta tgtggaggga acttattagt
    19261 atcagttata atgaatacta cagtaggcgt agaaaaaaca cactggccag taatgatgat
    19321 attaggactt gcacaaatta ttgtatattt tgtagtattt agttttttaa ttaagaaatt
    19381 taattataaa actccaggtc gtgaagatga gagtacagct acaggcgaag aacaatcaaa
    19441 acaacttaat ttagatgcag ggatagaaaa tataattgat gggttaggcg gaaaagaaaa
    19501 tattaataca gtagaaaatt gcatcactag attacgtgtt aatgtaaaag atgaaagtaa
    19561 gattaatgaa gatataatta atttaacgcc aaatagcggg attgtaagga aaggaaaaga
    19621 tatacaaata atttttggac ttcatgtgca tgaggtaaga cgtgcagtag aagatttttt
    19681 agaagcttat taaagatata tatttatata gcaagggtaa ataacagctt taagtgctgt
    19741 ttactattta ttaaagatac atataattta tagaggagga ataattatta tgattattac
    19801 tattgttggt ggaggaagca catttactcc aggtattgtt aagtcaattg cattaagaga
    19861 aaaagagtta gagcttgagg aaattcgttt atttgatatt gataaaaaac gtcaagataa
    19921 agtagctgta gttgtcaact ggatactaaa agaagatgtt aaaacagatg ttaaactaac
    19981 tgtaactaat tgtgaaaaag aagcgtatac agatgctaca tttatatttg cacaaatgcg
    20041 tgttggtaaa tatgaaatgc gtgagcagga tgaaaaaatt ccattgaaat atggttgtgt
    20101 aggtcaagaa acttgtggtt gtggagggat ggcatacgga ctgcgtacaa tacaaccaat
    20161 gataaaggtt attgatgatg tagagaaata tgctaaaaaa gattattgga tattaaatta
    20221 ctctaatcct gctgcaatcg tttctgaagc ttgtcgtaag ttaagaccaa atgcaaggat
    20281 tataaatatc tgtgatatgc caatagctat tatagatatg atagcggggt cattaaatat
    20341 caatgacgtt cataatatac gctatgatta ttttggtcta aatcattttg gctggtttac
    20401 ttcaatagat tataaacata gagacttaat ggaagagatt aaggaataca taaaagaaaa
    20461 taaaatatta ttaccaaaat cttatataga aaacttaaat aaaagtcgta gtacaggaaa
    20521 tcgccatgct gtaagtagct ggatacatgt ttgggaatct gtttatacaa tgttagaaag
    20581 cttcccaaaa tatctgccaa acacttattt aacatactat cttttaagta aagaaacagt
    20641 ggaacattca aacataaatc gtactcgtgc aaatgaggtt atggaaagcc gtgaaaaaga
    20701 cttatttgaa ggtgtgagac attacttaga aactggagaa atttctgaaa aagcatttta
    20761 tgcaggttca catggagatt ggattagtga tttggcagtt tcaattaaaa atgatacacg
    20821 tagtcgtttt ttagttataa ctgaaaatcg tggagctata ccaaacatgc catatgatgc
    20881 aatggtagaa attccagcat atattggtaa agatggtcca gaagttattg ctcgaggtcc
    20941 aatacctcta tttcaacaag gattaatgat gcaacaactt aacagtgaaa aattgatagt
    21001 agaagcttat atagaaaata gttatgaaaa agctttacaa gcattcacat taaataaaac
    21061 tattccaagt atgaatgttg ctaaagaaat tttggatgaa atgatagaag ttaataagga
    21121 atactggcca gaattaagat agtaggataa aagaaagggt ttttatttaa tcattagata
    21181 tgttttcaga gtcttttatc aaaatgtatt cacaaacata tggcaatata ttaaaataaa
    21241 gagtgatatt tttagaagaa tggggtgatg caatgaacat tataaataaa attttttggc
    21301 aagaagatag gattggcatg attacaaata atttagaggc agatacacat agccactgta
    21361 tgttacaact gttcttaggg atagaagaca gcattgaaat aacagttaat gaaaaattag
    21421 ttaaatgtaa ttgtatcata gtagataaaa atatctctca ttcattttca gcaagaaaaa
    21481 aagtttatta ctctgctatt attgaaccta catctatata tgcagaacag cttacctcaa
    21541 aaatgaatga ttttggctac tggatatgtg acaatgatgg attagagaaa ttacgtcaac
    21601 aaggaacttt tttgatatct aactctagta aagaacaata tttaagattt atggaaatgc
    21661 ttaataacta tttgaatata cctataactc tcaaacatta tgatgacaga attactgaat
    21721 tgttcaattt attacatact tgtaattgtg ataatcatac tatttcaagt tttgcagata
    21781 aagtgtctct atcagcaagt agattatcac atctgtttaa agaacaaata gggatacctt
    21841 taaaaagcta tatcttattt catcaaatgg agtgtgcttt tagagaatta ctttctggaa
    21901 aaaatgttac cgaagcttct atgactgctg gattcgatac tccatcacac tttgcaggga
    21961 cagttaagcg aatgatggga atgcctgttt ctttatcttt gaaagatagc gagtttttga
    22021 aagtatatta aatattctcg ttatataatg aagttgtact aagaaagggg ctttgttatg
    22081 aataaagaat atttagaaga aacaaaaatg ttaaattata atgaaccaca acttaagctt
    22141 ttggtatcat caaagaattg gttggaatta gatgattttc ataaaataaa atctatttat
    22201 gagtttgtac agaatgatat tttatttgga tataatgctt ctgatatgtt aagcgcaaca
    22261 caggtattaa atgatggtat gggacaatgc aatacaaaag ctactctttt aatggcatta
    22321 ttacgtgcag tgaatatccc ttgtaggctt catgcttttg atgtaacaaa agattttcaa
    22381 cgaggagcta catccaaatt gatttcacta ttagcaccaa aatatattct tcatacttgg
    22441 gtagaggtat tttatcaaga taggtggatt gctctggagg gtgtcataac tgataaaaaa
    22501 tatttagaag caattcagaa gaaatttttt aatcatggtg gaacgtttaa aaagtatgca
    22561 atagctacaa atgatttgaa aaatacatct attgattggg atggtaaaga tacttttatc
    22621 caaaaagaag ctatcgtgta tgattatggt atttttccat caccagacgt atttttttct
    22681 actcattcac aacatatgtc aaaattaaaa aatttcatat atgtacatct tatcagaaaa
    22741 ataatgacaa aaaatgtatg taaggctaga aacaattata ttgataaaaa tgaatgagtg
    22801 tttaagaggt aactatatag gaaatatata gaataattaa aactgtactg gttaaaatga
    22861 ataaaaagga gttatcacag agcaaactta aattgttcat aggataactc cttttctgta
    22921 ttaacttcta ataatttgat gtctttttat aaaacatata gatacaatta aaccagtaaa
    22981 tgcaagagta gctgctattg aaaatgcaac atctattcca tgaatgttag acagcatgtt
    23041 tgaactcata tgagctttct tagtagcact tgtcattata gtaataagta tagcagaacc
    23101 tatagctcca gatatctgac ggagtgtgtt attaattgct gtggcatgag atataagttc
    23161 tctatctaat gttttaattc cccatgtagt taatggcatt aaaaccattg aaataccaat
    23221 catacgaata caatacatca atgacacatg aattaaagat gtatctctgc ctaaaaaagc
    23281 gaaggataaa gttcctaaaa acaaacatcc tgttcctaat attgatagag catgaggtcc
    23341 atatttatct aaatgacgtc ctgcaattgg attgagtaca accatcaata tagcaccagg
    23401 taacattaat gaacctgaag ataatgcaga aaatcctctt acagattgaa cgtacagagg
    23461 aatcattaat gtagcagaca tcattgaggc atatacaatc ataattaata ttgtagaaat
    23521 aagaaatggt ttatttttaa aaaccctcaa ttctaaaaaa ggttctttag attttaattg
    23581 acgcaatgta aataaaatta atgacataat tcctattaat attggtaaat atgtggcaat
    23641 attagtccac ccataattac cttgattagt aaatccaatt aataaaccac caaaacctag
    23701 agatgatagt actattgaag gtatatctaa ttttagtttt tgagtttccc caacatttct
    23761 taataaaata aatgatagta taacatctaa tatagcaata ggggaaagaa acagaaataa
    23821 actatgccaa ccaaatgaat ctactaacca tcctgataaa gttggaccta ttgctggagc
    23881 aaatcctact gtaataccaa ctaaactcat agctgcacca cgtttttcct cagggcataa
    23941 atagagtata ataacttgta aaagtggcat tagagaacca gaacccattg cctgcaacac
    24001 acgagcaatc aacatagttg aaaaattatt agaaaaaagt gcaataatac atcctataga
    24061 aaagatagac attgatgtaa taaataactt tctagtacta aatcgattta ttaaataagc
    24121 tgttgtgggt acaataatac ctgcaataag tagataaatt gttgttaacc actgaccaac
    24181 atcagcagta attttaaaat ctgacattat attaggtaat gctgttgaca atagtgtttg
    24241 actgagagaa cttataaaag caccagtcat tacaattgct attattaaat ttcctttttt
    24301 attgttactt tgggtttcca taatttataa cgtctccttt tttaattgat ttttaatgct
    24361 gttcaataaa agtacttttg gtcttaaagt gtcttctttt tctaaatatg caatcattgc
    24421 atccataatt aaagaatcat attgttgatt ttttccctcg acgtataatt tttttattgc
    24481 ttctagtaat ttaggatttt caaggagaga agtatcagtt ttgattagtt cttctatatc
    24541 ttgtatcatt ttaaaaaata gcgatttatt atgaagggaa aagtaaactt catcttcttg
    24601 tttatgtaaa aacattgatt tttcaattaa aggatttaga ttatccatat tttctacaac
    24661 taaatctaga atttgtatag taaatttggc aataaagtat acatttattt ctcttttttg
    24721 tacatgaata atgtatataa actgatgaat tagagcatcc aataagaaaa ctaaatcaaa
    24781 tataattttt tctattttat caccatagtt atcaaataag cttaatttgt attcattcaa
    24841 taagttagct cgtgattttg tttgcaattt acttgtagcc ttacgttgct cttcagaaaa
    24901 attttctctt atataggtat ttatggtagt tctagaaaaa atatttttcc atacagcaat
    24961 tatctttctg tttaactttt ctgatgctgg aatgtcatta ttttcattta tggaatgtat
    25021 aatttctaaa aacttttcat cataataatt gattatttcg cagataaaac tttccttagt
    25081 ttcaaaatgc ttgtaaaaag tagctttaga cattttacac tcttttacaa catccgatat
    25141 tgaagtattt tgaaaaccct tttggtcaaa aattttttgt gcaatagtta taatgagcct
    25201 ttgtttttct gtcactttat tatctccttt cacgcaaaaa cgtttagtac tatagagtac
    25261 ctacaagttt taataaatat attatacatc atttggaaaa aaaatcaata tatagactgt
    25321 aatatgaaat ttttgaaaaa tttgttgtta tttttagtcg tataaaaaag aaacttaaga
    25381 gtttcttaag aattgtttaa agaagttgca ataaattagt ggtatgatta tagtatgaaa
    25441 gagaatgtct tgaaattttt ctgtatcaaa ttccaattat ggaggtgtag tatatgaaaa
    25501 agaaaatttg tgtagcaata atttttgctc tatttatagt ttttgtagaa atgtttattg
    25561 atagctctag cgaaggaagt aaaattatga cagctgaatt aagtaaatac aggaaaggcg
    25621 taaatgtatt atcaataaac aatattatag tagcgaataa aaaatatagt ttgcccaaag
    25681 attattctcc tcaggaaagc agtgaagcga gagatgcttt ttataaaatg aataaagatg
    25741 ctcaaaagtc aggattaaat ttaaaagctt ttagtacata tagaagttat gaatatcaag
    25801 atagattatt taagtcatat gtaaaagaac atggagaaaa agaagcaaat agattttcag
    25861 ctaaacctgg agagagtgag catcaaactg gacttgcttt tgatattgga ggagatgacc
    25921 agtcttgttg ggcaaataaa aagttcaaca atacaaaaga agctaagtgg ttgtatgaaa
    25981 atgcatataa atatggtttt atacttagat atccagaagg taaagagaat ataacaggat
    26041 atatgtatga atcttggcac tatagatatg ttggaactga acatagcaaa aactttgcaa
    26101 tgaataattt gactttagaa gaatatttac atataaatta agagatggtt tgttaaaatt
    26161 aggttaaaat aaaaaataac ctagactatt agctgtgtaa agtgatataa tgtaaataca
    26221 atttagataa ctactcgaag gggagtagct tacacgaaag tggtaattaa tcgtcaagac
    26281 agtagaaata ctcggttaat tagcaaggga ttccttgata gcaagacctt caaaataagc
    26341 attaggtatg tttgttttgg ggtctttttt tattcaagga atttttattt aaaaaaattt
    26401 aaatttcaag tatacataag agcccccaaa cataaaataa atttattgaa gaggagggca
    26461 acaaatgtct tacaaaagta cttatcaaga agtaattaaa aatttagact caagtttatc
    26521 tggtttgagt aaagaaagag cagaagagtt gttagaaaaa aatggagcta atgaattaaa
    26581 agaagcagat aaggttccta cttataagct tttcctagaa agttttaaag acccattagt
    26641 aataattcta ttgatagctg ctttagtaca aatattttta ggtgaaacag tggagtctat
    26701 aataattttt gcagttatta ttataaactc tgttctgggt gttgtacaaa caaaaaaagc
    26761 agaaagttca ttagaaagtt tgaaaaattt atctgcacca aacgcaaagg taataagaga
    26821 caataaaaaa atgacaattc cagcaaaaaa tctagtagtt ggagatattg tattcttaga
    26881 agctggcgat tacataccag cagatggaag gcttatagaa gcacaatctc tcaaggttgt
    26941 agaagggatg ttaactggag aatcagaacc agttttaaag catacagaaa aaataagtga
    27001 agatgtaagt ttaggtgacc agaaaaatat ggtatttagt ggtgctgtag ttgtttatgg
    27061 tagaggttct tttgtagtga ctggaacagg tatggatact gaaatgggta aaatagctgg
    27121 gcttttagaa agtgctgaaa ataaacttac accattgcaa caaaaaatag aggattttag
    27181 taaaaaactt ggtgtaggta ttgtaatact tgctctttta atatttataa ttcaagtagc
    27241 aagaaattac tttatggctg gaaatggaga aatgacacaa attattttag attcattcat
    27301 gttctctgta gcagtagcag tagcagctat accagaagct ctatcatcaa tagtcactat
    27361 agtattggct gttggaacaa atagtatggc aaaaaaacaa gccataataa gaaaattacc
    27421 agcagtagaa actttaggtt caacaagtgt aatatgtact gataaaacag gtactcttac
    27481 acaaaataaa atgacagtag ttgattatta tatgtatgga actaataatg atgagttaga
    27541 taaagaaaaa gtaaactatt cttatcatgc taaattattg actatggttt ctacattgtg
    27601 taatgactct catataacta gtgatggtaa agaagttggt gacccaacag aagtagcttt
    27661 aataaattat tcaagtaaaa atgatttaga ttatgataaa ttaagaagta agtatatcag
    27721 aataaatgaa ataccatttg actcagacag aaagcttatg tctactgtaa atagtatata
    27781 tggagattat ataatgttta caaaaggtgc tccagatatt gtgtttagta gatgtaaata
    27841 tgctttgaaa aatggttcaa aagtagatat aacagatgag ataatagaag aatataaaca
    27901 taaaaatgaa gagttttcaa atagagcact tagagtatta gcttttgcaa taaaagatgt
    27961 cccagaagaa gattttatac cttcaattga tgacgaacat gacatgacat tggttggaat
    28021 aatggcaatg atagacccac caagagaaga agtaatggat gcagtaaaag aggcaaagag
    28081 tgctggtata aaaactgtta tgataactgg tgaccataaa acaacagcag cagcaattgc
    28141 aaaagagatt ggtataatgg aagaaggaga cttggctctt actggaaaag agcttgatgc
    28201 tttgagtgaa gaacaactat atgaaaaact agaaaatata agtgtatatg ccagagtctc
    28261 gccagaaaat aaaattcgta tagtaaaagc atggcaacat aagaataata taacagcaat
    28321 gactggtgat ggtgttaatg atgcacctgc attaaaacaa gcagatatag gaatcggtat
    28381 gggaagtggt actgatgtag caaaagatgc ttctgctatg gtattggttg atgataactt
    28441 tgcaagtata gtaaatgctg ttgaagttgg tagaacagta tacagtaata taataaaatc
    28501 aattacttat ctatttgcag gaaacttagg agctatagta tccatagtat ttgcagtgtt
    28561 tgcaggttgg tcaaatccat ttacagcttt acaacttttg tttataaact tagtaaatga
    28621 ctctttacca gcaattgcat tagggtttga aaagtcagaa aaaaatataa tgaataaacc
    28681 tcctagacac ccggatgaaa gtatactagc aggaggaaca ttgaaggcag ttgctataag
    28741 aggttgtata ataggtgtag ttacaataat tgcacaatat ataggattac aagtatctcc
    28801 agaacttgga gtagcaatgg cattttcaac attgatatta gctagaataa tgcaaacatt
    28861 acctgcacgt tctaataatc aaacagtaat aaaattaggt tttttcagca ataaatacgt
    28921 tataggagca gttttagtct gtcttgggct ttactcttta acactaatac cagtgctaag
    28981 aggaatattc tctataccaa caacgtttgg attgtaccag ttggggattt gcctagggtt
    29041 agcattaatt tcatcaatat taatggaact taacaaatta ataaatagaa gtgaagtaaa
    29101 ataatcaaaa aattaaaggg catattgatt ttaacaatat gccttttata aatagtctaa
    29161 tttattctat attatcgatt atttggaatg cttttataac ttcaggtaat tctttttctt
    29221 caggcattct aaatcttatg aaatttttat caagtccagt aaaatcactt ccagatataa
    29281 ctcttatttt taatttagca aactcttgtt gtaaatctat gtccttgttt ttatgttcaa
    29341 ctgtcataat tgatacagta tcactagttt cagctatatt aaggtttttc catggaatta
    29401 ataattgatt ttttatatct tttgtttttt cttttaattc ttctataaat tgaacatcat
    29461 ctaataaatt tgctgcaata ctacgagata tttcagacac ttcgtaagga gtagaaatct
    29521 ttttaatagg gctaacaagt tgttctggaa gaactgcata tcctgctctt agtcctgcta
    29581 aaccgaatcc ttttgaaaat gtttttaaag caattacatt atcataatta tttagaagct
    29641 tgacagctga attttctttt ggcatgtatt caccataagc ttcatctacc ataactgcta
    29701 tatcatattt agctgcttcc tttactatat tttcaattga tgataaatgt ataatttgtc
    29761 ctgtaggatt gtttggattg tctatgtaaa ttactttata ttctggattt attttttcaa
    29821 taaattcttt ttcattaaac ttgaaattat cctctttctt taaaagaaca taatcataag
    29881 ttgcaccatg catttttata tctgtctcat attctgagaa ctgaggtgaa tatcctaaaa
    29941 ctttatctcc cttttcaata aatagtctat ttaaaaggta aataacatgt atagaaccat
    30001 ctgccaaaca aattctgtca gtatctaatg ctataaaatc tttccaataa tcgactatat
    30061 tatcttttaa agcaattgaa tgaggatatc ctcttatcat atcaaatttt aaagcattaa
    30121 aagcttctac agctttgttt gagtaagaca cggtatttat accttcacca cagtctatct
    30181 cgaatacatc ttctgaatgt ccagattcta ttgcataact ttttttcaac tgcatttcaa
    30241 tagctttgtt aatttttagt ttcatacttt atcactcctt atttagtcaa taataaatgt
    30301 tataa
//
LOCUS       DAEMGG010000041        30236 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEMGG010000041 DAEMGG010000000
VERSION     DAEMGG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.041"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(189..794)
                     /locus_tag="KPK24_001700"
     CDS             complement(189..794)
                     /locus_tag="KPK24_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2299374.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPFVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(918..1035)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1095..2261)
                     /locus_tag="KPK24_001701"
     CDS             complement(1095..2261)
                     /locus_tag="KPK24_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2299375.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2409..4550)
                     /locus_tag="KPK24_001702"
     CDS             complement(2409..4550)
                     /locus_tag="KPK24_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG2299376.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DNEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKTIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNICEIDEKIKEIGLRELSEKLEVGQVTLKDIITEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4694..5801)
                     /gene="prfB"
                     /locus_tag="KPK24_001703"
     CDS             complement(join(4694..5725,5727..5801))
                     /gene="prfB"
                     /locus_tag="KPK24_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG2299377.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(5816..8491)
                     /gene="secA"
                     /locus_tag="KPK24_001704"
     CDS             complement(5816..8491)
                     /gene="secA"
                     /locus_tag="KPK24_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG2299378.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8623..9174)
                     /gene="raiA"
                     /locus_tag="KPK24_001705"
     CDS             complement(8623..9174)
                     /gene="raiA"
                     /locus_tag="KPK24_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG2299379.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9727..10401)
                     /locus_tag="KPK24_001706"
     CDS             complement(9727..10401)
                     /locus_tag="KPK24_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG2299380.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(10404..11456)
                     /locus_tag="KPK24_001707"
     CDS             complement(10404..11456)
                     /locus_tag="KPK24_001707"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG2299381.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11505..12464)
                     /locus_tag="KPK24_001708"
     CDS             complement(11505..12464)
                     /locus_tag="KPK24_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG2299382.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12557..13078)
                     /locus_tag="KPK24_001709"
     CDS             complement(12557..13078)
                     /locus_tag="KPK24_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG2299383.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            complement(13089..14423)
                     /gene="celB"
                     /locus_tag="KPK24_001710"
     CDS             complement(13089..14423)
                     /gene="celB"
                     /locus_tag="KPK24_001710"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG2299384.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(14462..14788)
                     /locus_tag="KPK24_001711"
     CDS             complement(14462..14788)
                     /locus_tag="KPK24_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2299385.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14812..15120)
                     /locus_tag="KPK24_001712"
     CDS             complement(14812..15120)
                     /locus_tag="KPK24_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2299386.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15261..17174)
                     /locus_tag="KPK24_001713"
     CDS             complement(15261..17174)
                     /locus_tag="KPK24_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2299387.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(17506..18288)
                     /locus_tag="KPK24_001714"
     CDS             complement(17506..18288)
                     /locus_tag="KPK24_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG2299388.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKIVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKDNVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18291..20510)
                     /locus_tag="KPK24_001715"
     CDS             complement(18291..20510)
                     /locus_tag="KPK24_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG2299389.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20774..21871)
                     /locus_tag="KPK24_001716"
     CDS             complement(20774..21871)
                     /locus_tag="KPK24_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299390.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTGGYKENKSEIS"
     gene            complement(22187..23380)
                     /locus_tag="KPK24_001717"
     CDS             complement(22187..23380)
                     /locus_tag="KPK24_001717"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG2299391.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23517..23620)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23809..24318
                     /gene="yyaC"
                     /locus_tag="KPK24_001718"
     CDS             23809..24318
                     /gene="yyaC"
                     /locus_tag="KPK24_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG2299392.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(24429..24857)
                     /gene="fabZ"
                     /locus_tag="KPK24_001719"
     CDS             complement(24429..24857)
                     /gene="fabZ"
                     /locus_tag="KPK24_001719"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG2299393.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24873..25892)
                     /locus_tag="KPK24_001720"
     CDS             complement(24873..25892)
                     /locus_tag="KPK24_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG2299394.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(26089..26349)
                     /gene="spoIIID"
                     /locus_tag="KPK24_001721"
     CDS             complement(26089..26349)
                     /gene="spoIIID"
                     /locus_tag="KPK24_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG2299395.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(26447..27115)
                     /locus_tag="KPK24_001722"
     CDS             complement(26447..27115)
                     /locus_tag="KPK24_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG2299396.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(27181..28245)
                     /gene="spoIID"
                     /locus_tag="KPK24_001723"
     CDS             complement(27181..28245)
                     /gene="spoIID"
                     /locus_tag="KPK24_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG2299397.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(28332..29585)
                     /gene="murA"
                     /locus_tag="KPK24_001724"
     CDS             complement(28332..29585)
                     /gene="murA"
                     /locus_tag="KPK24_001724"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG2299398.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(29606..>30236)
                     /locus_tag="KPK24_001725"
     CDS             complement(29606..>30236)
                     /locus_tag="KPK24_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG2299399.1"
                     /translation="ALEISEANFKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDK
                     KGINWNQKGSGDKIQLCATIKGSDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIY
                     SNVENVLNKYSSQVDVSACIVGEYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFL
                     SITAYSKVLNDYELEYLGEKINLNIGIKYNEDDGKTLIYIATPIIKLDY"
BASE COUNT         8991 a         5691 c         3201 g        12353 t
ORIGIN      
        1 tagttaatcg ttttaattct tcctttattt taaaatttaa atttgtctaa atatttctta
       61 ttttatatac attttttgat aaattaataa agccataata tatttaatat atcaatcaca
      121 atataggact gaagttataa ataaattatg gctattcaat gcatttaatt ttatattttc
      181 ttgtctaatt atttctttat tatatttccc atttttttat aaagtggcaa tgttactaaa
      241 gatattataa cagccttcag cacattaaat ggagctatca tccaaattac aaaagtgaat
      301 aaatcatgaa cttgaggatt tatagctgaa cctaaaccta ttatagcatc tagtcccata
      361 agttgaccat aaagtggcaa cattacaaaa taatttacta atgaaccaat tatagtcata
      421 gatattgttc ctaatacaaa accaatcaat acacctttta aatctccact tctcttatat
      481 gaataacaaa gtatcattac ataagaacca ccaattaata tatttgcaaa ttctcctatt
      541 cctccagttg ttgttgctgt tattgcttgt aacacatttt ttactacaac tattccaaat
      601 ccaacaaatg gtcctagaga cattcctccg aatattgctg gaatatctga taagtcaatt
      661 tttaagaaat ctggaaatat acctggtatt ggtgcttgaa taaacattaa taaataagat
      721 attgcagata aaattgacat tactactaaa gttcttgttg aaatcatatt gcctcttttg
      781 gctgtgtttt gcataattat ttccccctaa tcaatatgtt tatatttttt aggaaaaaca
      841 aaaaactcga agaccctatc ttcgagtttt tatggatata gtttaaacaa actaaaataa
      901 acttatttat cttccataaa tatcttctct catccagact ttactgtcgg ctttggaatc
      961 tcaccaaatc aaccacttaa gtagttcgcg ggctataccg ccggtaggga atttcgccct
     1021 gccccgaaga tcttctttga ttttttaatt ttccttacac tcttatatta gttaattttt
     1081 taataattgt caagttattt tttctcatac actactttgc catttattat agtgtaaagt
     1141 acattacttt gaatctctag tggagaatta tcccaaatta ctaggtctgc atctttacca
     1201 acttctatag agcctactct atcttctatt cctaaagtct tagctggatt tatcgtaatt
     1261 gattctattg ctttttcttc tttcatacca tgtttgactg caatacctgc acatattgga
     1321 agatattgaa ctggtattac tggatggtct gtcattatac atacatcaag acctgcattt
     1381 gaaagtattc ctgctgtatt aaatgtcaaa tttctaagct caaatttaga cctttctgac
     1441 aaagatggcc ctactattac aggaaaccct tcttccacta attcatctac aataagatga
     1501 ccttctgtac aatggtctaa tgtaagcttt aaattaaatt cttttgcaat tcttattgct
     1561 gtaaacatat catctgctct atgagcatgt gccttaaaag gaatttctct tcttaaaact
     1621 ggtattaaac tttccatttt tatatcatat tcaggttttt cacagtcttc atcatcatga
     1681 ctctcataca tatcaatatc ctctaaatat tcttctgctt tttttaggtt ttctctaagt
     1741 aaagcagcta tagccattct agtttgagga gatttatcat cctgaccata acagctcttt
     1801 ggattttctc caaaagcgat tttagatgca actggatttt ttattaccat tttatcaatt
     1861 ctttttccac atgtctttat agctatacat tgaccaccca taacatttgc acttccaggg
     1921 gttgtacaaa cagaagttat tccaccttca aaagcttctt taaaagttct atccattgga
     1981 ttaattccat ctatgggatt taattgtggt gttattgggt cagtctcttc atttccatct
     2041 gctccttcaa accctatacc atcttcccac aaacctaaat gagtatgagc atctataaat
     2101 ccaggaaaca caagtttatt actagcatct attacctcta catctaaagg agctatcaaa
     2161 tcttctccaa tttctattat ttttccttca tctataagta tatctccatg aattatccca
     2221 tttgttatgg tatttatttt accatttttt ataaaaatca ttcattttcc tcctgatttt
     2281 caagactatc gttcttattt taatatattt tcttactgta tacttttaca aatttttaac
     2341 atttatacac tcgagtaacc tcatttacaa acatagaatt taagtactat aaccatacat
     2401 aaacttatct attttttcat tgataaggca actctttttt tgtttaagtc aattccaatg
     2461 acttttacat ctactatatc acctactgta acaatactca tagggtcttt cacaaaacta
     2521 ttactcattt ctgatttatg aactaatccg tcatttttta ttccaatatc tacaaatgca
     2581 ccaaaatcta caacatttct tattgtacct tttaaagtca taccctcttg tatatcttca
     2641 atttttaaga catcagttct aagtataggt tttatacctt cttctcttgg gtctcttcct
     2701 ggttttttga tttcagttat gatatctttt aacgtaactt gccctacttc cagtttttca
     2761 cttaattctc tcaagccaat ttcttttatt ttttcatcta tttcacatat attcttattc
     2821 tttacatcat ctaaagaata tccaattatc tcaatcattt ttttacatat atcataactt
     2881 tcaggatgca cacctgtatt atctagtgga tttttaccct ctaagattct cataaatcca
     2941 gcacattgag taaatgcttg tggtcctagt ctctttactt ttttcaattg tgctcttgac
     3001 gtaaaatctc cattttcttc tctatatgct attatatttt ttgctatagt tttacttatt
     3061 cctgcaacat gttctagtag ggagtatgat gcagtattta aatctactcc aacactattt
     3121 acagaatctt caacaactcc atctagtact tcttctaatc tctttttgtt taaatcatgc
     3181 tgatattgac caacgccaat acttttaggg tctattttta caagctctgc taatgggtct
     3241 tgtaatcttc tagctataga tatagctcct cttattgata catttatatc tggatgttcc
     3301 tcatttgcaa gttcagaggc tgaataaaca gatgctcctg cttcactaac tatcacatac
     3361 tgcacttcgt tatctatttc ttttatcatt tctgatacaa aagtttctga ttctctagat
     3421 gcagtaccat ttccaattga aattatatct atatcgtatt tttcaatcaa tccttttaaa
     3481 actttctttg ctccctctac atcattttga gggtctgttg gatatactgt tgtataatct
     3541 agtagtttac catttttatc tactacagct attttacatc cagttctaaa tgcagggtca
     3601 aatcccataa ctactttgtc tttaactggt ggttgaagca ataaactttt aacattcttt
     3661 ccaaatacac ttatagctct ttcttgagct atttctgtta aatgatttct tatttctctt
     3721 tctattgatg ggaaaattaa tcttttataa gaatcttcta ttgaacttac gatttcttct
     3781 ttatttttga aatttttatc atttacatat tcgtttatta tgtaatttaa aactttatca
     3841 ttatttattt ccaacttaac ctttaggaaa ctttcttttt ctcctctgtt tattgccaaa
     3901 actctatgtg gagccatact ttttactgct tcgctataat cataatacat atcataaacc
     3961 gatttctcat cagtagcact tttgctaact atcatacctt ctcttaaagc taattctctt
     4021 atatactttc taatcttagc atcatctgag actagttctg caattatatc tctagcacct
     4081 tttagagcat cttcaataga aagaacttct ttctcttcat caagataatt ctttgcctct
     4141 atttctatat tatctaaatt attctctagt attgacaatg ctaaattttc taatcctttt
     4201 tcttttgcta ttgttgctct agttcttttc ttttgtttat atggagcata tatatcttct
     4261 acttcttgta atgtttttgc tttttctata ttttgtgtta tttcatctgt taatttccct
     4321 tgttcatcta ttagtctaac tacatcatct tttctacttt gaagatttct caaatacatt
     4381 aatttttcat aaaattctct tagtgtaaca tcactcattt cgccagtcat ttcttttcta
     4441 tatctagcta taaatggtat tgtatttcct tcatcaataa gttttaatgt attatttatt
     4501 tgttcatctc taagattaaa ctcttttttt aagatttgat ttatatccaa tttattcact
     4561 cctaatgtta gtttttcata agtataacaa aacagaacga aggctgaggg ttatcgtcct
     4621 cagcctaagt gctgtaagtg catattaagc actaaccttc ttatacatat atcttaatta
     4681 cagtgcatat ttattaaact attttattca tttttaagta ttcattaata aacaagtcta
     4741 tatttccatt cattacacta tctacatttc caaactcagc atttgttcta tggtccttaa
     4801 ccaatttata tggttgaaaa acatatgacc taatttgact accccaagtt atttgtgagt
     4861 atttgccttg tatatcttct attttttcct tttgttctaa ctcttttaat tctatcaatt
     4921 tcgccattaa aagtctcata gctctgtcct tatttagatg ctgagacctt tcattttggc
     4981 actgaactac tacaccagta gggatatgtg ttattctaac tgctgaatca gtcgtattta
     5041 catgttgccc tccagctcca gaagctctat atgtgtctat ctttaaatct gatggattaa
     5101 tttctacttc tatattttca tctaactctg gtgttacatc tatagatgca aatgaagtat
     5161 gtctttttcc tgatgggtca aatggagata ttctcactaa tctatgaact cctttttcac
     5221 tctttaaata cccataagca tttgttcctt ctacaagaat tgtagctgtc tttatccctg
     5281 cttcagggtc tgatattata tctaaaagct taactttata acccttactt tctgaccatc
     5341 ttatatacat tctcaaaagc atttgagccc aatcttgagc atctaagcca ccagtaccag
     5401 catttataga taatatagca ttatttttat catactctcc atttagaaga gtctttattt
     5461 tcatttcaga taatttttct tccattgatt ctatggactt ttctatatct ctttctacag
     5521 aatcatcatc ttcttctaaa cctatttcaa ttaatacttc tatatcttct aataagcttt
     5581 ttaagctttc atattcttct aatgtctctt ttaaagattt attttcttga agtactcttt
     5641 gagcgacttc attatcattc caaaagtctt gttgattcat cttttcttca ttaatttgta
     5701 tatttttcat caatgaagct atgtcaaaga gaaaccctca attcttctaa atttgaagcc
     5761 atctgctcca tactatgtct atactcttgt aactttaaca tttaatcctc cgagattatc
     5821 ttccacaaca gtttttatat tttttaccac ttccacaagg gcataagtca tttcttccaa
     5881 ccttttcatc ttttttaaca gttttacttg ttggaagtgt tccatctgat ggagaagata
     5941 gattttcaac atctacaact gcttttcttt ccactggagt ttctattgtt atattaaata
     6001 aatatcttac agtatcttct ttgatatgtt tattcatttc atcaaacata tcaaaacctt
     6061 ccattttata agcaataact gggtcttgct gacctacagc acgaagacca ataccttggc
     6121 gtaattggtc catagcatct atatggtcta tccaatggtt atcaacagct tgaagtaaga
     6181 ttactctctc aacttctctc attctctcat agccaacttg ttcttctttt gaagtatata
     6241 tcttcatagc tatttcataa actttttctg ttatttcaac tggattaagt ttttctattt
     6301 ctggtatttc tatgctacct tttggcaaga acaaattata catatgttct ttgaatcctt
     6361 cttcatcaaa tcctttatct tgtgtataaa gagtaactgc ttcttctatt atagaatgag
     6421 tcattgattg tatttgttct tgtaaatctt caccttctaa tacacgtttt ctctctgcat
     6481 atattatttc tctttgttta ttcataacat catcatattg aagtacatgc tttcttattc
     6541 caaagttttt accttcgact ttcttttgtg ctccttcaat agacttagat aatattctat
     6601 gttctatcgg catgtcttct tcaagaccta ttttatctac aataccagat attctatcac
     6661 taccaaacaa tctcataagg tcatcatcaa gacctatata gaatcttgaa ctccctgggt
     6721 caccttgacg tccagcacga cctctcaact ggttatctat tcttcgagat tcgtgtcttt
     6781 ctgtaccgat tatagcaagt ccacctgctt ctacaacttg tttttgttct tcttgtgttt
     6841 gttgcttaaa cttttcaaat agtttttcat actcttctct agcttcaaat agaatttcat
     6901 taccttttac aggtatacct tctattggag tatctactct atttactatt tcttctttaa
     6961 atccatttct tctcatttct ctcttagtta aaaagtctgg atttccacct agaactatat
     7021 ctgtaccacg accagccata ttagtagcta ttgtaacagc tccaagtcta ccagcttgtg
     7081 ctattatttc tgcctcttta tcatgatgct tagcatttaa tacctcgtgc tttatgccct
     7141 ttttctttag tatttgtgat aataattctg atttttctat tgatactgta cctacaagta
     7201 ttggttgatt gactttgtgc ctttcaatta tttcttgtgc tacagcatta aatttaccta
     7261 tttcactttt gtatacacaa tctggtaaat cttcccttat cataagctta ttagtaggta
     7321 cttggaaaac atccattttg tagattgctt taaactcttc ttcttctgtt tttgctgtac
     7381 cagtcatacc agaaagtttt ttgtacattc taaagtagtt ttggaatgtt acagtagcta
     7441 gtgttttaga ttctctttgt attttaagac cttctttagc ctctattgct tgatgtaaac
     7501 cttctgaata tcttctacca aacataagtc tacctgtaaa ctcatctact attactattt
     7561 ctccatcttt agcaacataa tcaacatctt tcttcattat tacatgtgct cttaaagctt
     7621 gatttatatg atggtataat tctgtatgag atatatctgt tatattatca acattaaaat
     7681 acacttctgc tttttgtata cccgatgctg ttagagatac agccttatct ttttcttcta
     7741 attcataatc atctggtttt aaggttaaaa caaatgtgtt tgcatctgaa tataagtgag
     7801 ttgatttgtc tccaggtcca gatatgataa gtggtgttct agcctcatct attagtatag
     7861 agtcaacctc atccactata gcataattta atcctctttg aactctttgc tctttgtgta
     7921 ttaccatatt gtcctttaga tagtcaaatc catattcatt atttgtacca taagttatat
     7981 cacaatcata ttgttctttt ctaacttttg gattttgacc gtgtataata acgcctacac
     8041 tcattcctaa aaactcatag atttttgcca tttggtctct atcacgtttt gcaaggtaat
     8101 catttactgt tacaacatgt acaccttttc cagtaagcgc attcaaataa acaggagcag
     8161 ttgcaaccaa agttttacct tcacctgttt tcatttctgc tattcttcct tgatgtagta
     8221 ctatacctcc aatcatttga actctgtagt gtctaagtcc taacactctc ttagaaactt
     8281 ctctaactac tgcaaaagct tctggaagta tatcatctat actttcacca tttgctaatc
     8341 tttctttaaa tatatttgtc atatttttaa gttccgagtc tgccatagat tcaaatttag
     8401 gttctaatga atctattata tcaacagttt tattgaactt ttttaattct tttttatctg
     8461 ccatgttgaa taaattatcc ataaagctca tattttacat ctctcctcgt tctttattgt
     8521 actattattc attttaccat aaaaactata cttttaatat taatttaaaa agctatcgat
     8581 aatttattta tcgatagcta atatgattaa atctaaattg ctttattcat gttctattat
     8641 accatatcca ccattattac gcttataaac tactgctatt tcatctgaat ctatgttctt
     8701 gaacatataa aaatcatgtc ctattaattc catctgtaat acagcttctt cttcactcat
     8761 tggcttaaca ctaaatttct tacgtctttg aattactaat tcttgatttt catccccata
     8821 atcctcttgt tcatcagcta gatttatctc tgcatcttgg aatcttatac tcttgttgtc
     8881 tttatgttta tcttgtaatc tctttttata tttttttaat tgtttactta atttatcata
     8941 tacaacatct atagctgtgt ataaattttc ctgcatatct tctgctctaa ttataggacc
     9001 atttacagta gctattgtta cttctatttt ttgtcttgct tttctcgcac ttactgtaac
     9061 ttttacatca gtgtctggat gaatgtaatt atctaactta cttagtttag tttcgatttt
     9121 attttttatt gcatcagtta attcgatttg ttttccagat ataattatgt tcataaatac
     9181 atctcccttc aattatgcca gtataagaaa atttatactt taataatata ttctacacga
     9241 actctcataa tcctcttttt attatttact tttatgattt tttctattca attgatagaa
     9301 ttatttatca tttttagtta cattgatact tatgtggatt aatattatta caaacacctg
     9361 tggataatgt ggataaaact gttaataact cttgtttttg tttatttaaa gctattttta
     9421 ctcctatttc tatccacaaa aatctccatt aattgtttat taatatgtga attcatgtca
     9481 taagatggta attttaggaa attatgtgta ttatatgcca atataccttt ataatttttt
     9541 aataaatata aaccctctaa aacctccata atctatcttt tctttcatta tttatattta
     9601 aaactactta aaagttataa agcatactca acaataattt catatttaat acctattaac
     9661 attcatatac ccaaattata attaacttac tctctttatt taaactaagt gttttatatt
     9721 catatactat cttgaaatta tatttacgag ttgtttaagc ttttgtctat ctatatcttc
     9781 aaaaggactg ttatttattc taatcgctgt accaaaatga aaattacttg ccttagttaa
     9841 ttcctttatc ttttctatat tattaaaatt aagacctcca cctaaaagaa tttttatgtg
     9901 attagaacta agcatcatat tctttatcat ttgtatgtta tcaactatac ttcctttccc
     9961 tcctgatgtt aatatattcg taattttatc ataatccttc aaaattttaa cactatctat
    10021 tgtatttgat tcatctatgg ctctatgaaa tgtaacatca agattatcac aacatttcaa
    10081 taaaacattt agatttttct catctatact attgttttta tccaatacac caaatacaac
    10141 tccattagca ccaatctctt taaccacacc tatatctttt tgcattatac ttatatcttc
    10201 tttactgtat acaaaacttt tcgcatgatg tcttatcatt acgttcacag gtatctttac
    10261 actgttaaca acactttcta taagaccaaa acttggtgtc aatccacctt cagttaaagc
    10321 actaactaac tctattctat ctgcaccaca atcttctata attttagcgt cttctactga
    10381 cattcctatt atttctaaca taactactcc tttgctaatt catatattgc atttgcaaat
    10441 atctttgcat ttaatattat atcttctata tccatgtatt catcaggatt atgagctatt
    10501 cctttttgtc ctggaaatga gggtccaaac ggaactatat ttggcattac ttttgcatac
    10561 gtacctccat tagttgttac tggagttcca tctaatgatg taaccctttc ataagctagc
    10621 tgaagctttt taacaagatt actctcttta tcaaaatata caggattaaa attagaaatt
    10681 acctctatat cgatactatt tggaaaacta gcttttattt catctaatac atctttcaat
    10741 ttataactaa aatcgtattt tacctttata tctaatatag tatcactatt ttctatattc
    10801 agctctttat atgtaacaag cacattttca gacatatttt ctataaaacc taggtataca
    10861 tcttcttcat aattgatttt actttttatt ctaatctttg ccatgccttt ttcaccatat
    10921 acaacaggat acttacaatc tggtgtaaat cccataatag gtgctttttc tttttctaaa
    10981 taatatggta tatcttcaaa tccactttct tcatttgtac caaatattat tctaactctc
    11041 ttatctaatt ttaagtttaa ttctttaata gcatataatc catataaggc agacattata
    11101 ggtcctttat tgtctagtac tcctcgtcca taaattcttc cattagtttc ttctccctta
    11161 taaggctgat gcttccaacc atctccttca tgtacaacat ctacatgtcc aattacacaa
    11221 acataatcat cactatctcc atgctctgca taaccaatat aattatccaa gtttctaacc
    11281 ttaaatccca atttttctga tatttctaat gctttattaa gtgcctcccc tactttttcg
    11341 ccaaaaggga agccattttc tgactcccct ttgacactag gtatttttac aatatctata
    11401 atatcactta ataaatcttc ttttaactca tctattttat tacttatgat attcataaaa
    11461 atctccttat tataaaactc tattttaatt atgtaaccac tactttactc acttacttct
    11521 attggtgctt ttattctagc ctcatatgct tccatccact catttgatat aaccttatgt
    11581 atagtctttc catctactct atttgaaata tatacagtag gagattcttc tttagttact
    11641 actgaaaaag taaaatcagc tatatttgta gctactcccc attcaccttt attattcatg
    11701 gcaactaatg ataaatcacc agcttttccc cttcttttga taagctgttt atcaagttca
    11761 tttactgcaa tatcacatgc ttcttgtgga tgcttccctt ctttcataag tctaactatc
    11821 tcataagata tacatccctt cattaaatct tctcccaaac cagtagctgt agctcctcct
    11881 atactactat caacatagaa tcctgaacca gatagtgcag aatctcctat tctacctctt
    11941 ttcttcataa acaatccact tgttgatgta gctgcacaca tttttccagt tttgtctaaa
    12001 gatatcatac caacagtatc atgaccagca tatgggctta atcctttgtc taaagtctct
    12061 ttttttctct ttttataatg taacttcgct ctatcagtca acatattttt tctttcaaat
    12121 ccatttttat gagcataatc ttcagcacct attccaacta aaaaattatt taccttctca
    12181 taacttaact tcctagctat gctgacagga ttagaataat ctcttatacc agcaactgcg
    12241 ccaatatcta atgtgttacc atccataaat gcagcgtcaa gttctacctc acaattttca
    12301 tttggaagtc caccatatcc aaccgattta tagaatggat agtcttcaac tcttcttaca
    12361 gcaagctcta cagcatcacc tgcttccatg cctttttcta aatgttctga tgcctcagtt
    12421 attccttcta gggccattct ccatgtcgct atcattcccc acatgttaaa attcccctcc
    12481 ttaaattata tagattttac taatgtatgc ttattataaa tagtataata tttaaacttg
    12541 ctaataatta atactcttat tgataacaaa actcttcttt catcaaagtt atatcttctt
    12601 tatttgctat tgcttttgcc aatttacata tgttatttaa tgaatcatta agaccaattc
    12661 cacctttttt tggagtctta actatacata tgtcattttt atattcgttt atcacatcta
    12721 tattttcttc tgacatagct gcaaaagctg gtatatctgt tttatcattt atatttttag
    12781 acagtattgc tgacatctta gaaaagtttt cgtaattaaa gcttggtcca cagattacta
    12841 catcagggtt taattttttt accatagcta tcatcttact cgttacttct tcttgattat
    12901 ttacaaaaaa ttcatcccca caaaataagg tagctattac cttagattca tttaaaaatt
    12961 gctccatcat tacacctggt cctaatgggc ttgattttcc agcaggaggt atattacttt
    13021 tctcttttcc tccagctcca gcttgtattt ggtccaatat cataactatt ttcttcataa
    13081 tcttcctcct ataatactaa atcatcaaaa gatatatcat catcttcctc ttcaacagtt
    13141 tcattttctt ctctcaagta ttgcttatct agcattttta taaatggtat atacataaat
    13201 attcctagta taagaagtac taattgtaag actgcacctt gccatcctgt tgttaaaaaa
    13261 ccagagaata taattggagt tgtccacggt aactgaactc catttgtaag aggcactaat
    13321 cctgctgaca tacagaagta agctattatt atatttattg ttggaactat tgcaaatggt
    13381 atcaatagta ttgggttaag cattattggt agaccaaata tcaatggctc atttatacca
    13441 aatattcctg gtaaaattga tagttttccc attgacttta tacgtttact tttacatatt
    13501 aataacatag ctatgactaa tgatagtgta ctacctgctc caccaaatgt cgcaaacata
    13561 tcttggaatt gacctgttat tatgtttggt atttttgaac cagcttgaaa agcagctaag
    13621 ttttcagctg atagaatttt taatattgga ttatacacag ccccaacaac actacttcca
    13681 tttattccaa agaaccagaa taaatgtaag aatatataag cgattgccat agcacctaaa
    13741 ctatttccta attttaataa tggcatttgt aaaaacttaa atataaattc atgtatactt
    13801 ccatatggtg tagcttcaaa agctatctta actaaaaaga aaactaccat aactattgca
    13861 cttggtatta atgctgcaaa tgattttgat acagttggag gaactccatc tggcattttc
    13921 aatgtccatc ctttttttac aactgcagca aataatttta cagaggttat tgctgttatc
    13981 atacctacaa acattccctt agaacccatc caacctagtg gtatacctgt taccatgtaa
    14041 actgattctg ttccttctgg tgtatatggt attacaaatg gtgttattat aaaaaaggca
    14101 actattgcaa ctgctgctga tgctattggg tctacatcta tttgctttgc ataagaatat
    14161 ccaatcccta aaactgcaag tagagtcatt acttcaaatg ttgctacagt tggttgttgc
    14221 aattttacag cccaatctgg accaaaaatt tgacctacaa attcattcca tccttttata
    14281 ggaaaatttg ctactaataa aaataatgaa cctattatta ataatggtga tgatactaaa
    14341 aaaccatctc taattgcaat taataattta tttttaccta ttttttcagc caatggcata
    14401 agtattcttt ccaatctatt catctatcca ccctcatttc ctattatttt ttactataat
    14461 tctacgcttg tttagatttt attagcttta tagcaagttt taatactttt tccccattca
    14521 tagttccata atcaacttca ttgattatat ctataagttt tccttgagct tcaaactttg
    14581 gttttaactc ctttagcatg tatttaactt gtggtcctaa taatatacaa tctggatttt
    14641 tttcttcaac tattttgtca atttctgcta tcggaaatgc ctctacttct attggtaaat
    14701 tgtgttcatc tgctaccttc tgcattttat ctgcaagtaa acttgttgac ataccaaagc
    14761 tacaaaataa atatactttt cttttcattt tttaaatccc ccttaatttt tttattttaa
    14821 tttttgaatt tcttttctta acataatcat ttcttttata aggtttctct cactcataca
    14881 agtcattaaa tggtcttgag agtgaatcaa taaaatagat atatctgatt tatctccccc
    14941 agcttccttt tgtataagtt cagtttgtac tttatgtgct tttaaaattt cttcatttgc
    15001 attttcaatt aattcatctg cttttttaaa atcgttttcc tttgcatagt caagtgcttc
    15061 ataaagcata gattttgcat ttccagcatg aattataatc tttaaaacta tttcttccat
    15121 tttttcctcc taagttgctt ctctactttt agattattta taccttacaa acatataata
    15181 tcttataaaa acaaaaaaac atgcacaact ttttccatca catggaaaat tttgtgctta
    15241 tccatagttc actatgaatt ttatataaga atatctttta ccttttcaat gtcacattta
    15301 cttagaatat ctagcatatt ttcatcctct gttaaattag atatttcttt aaataattct
    15361 ctaaatactt ttctatcatc atctttttta tacgctaaca aaagtataat ctttatatcc
    15421 tttccatttc taatttttat tggtgttttt aaccttgcaa aacataaaac agattgtttt
    15481 atatatttca tgtctccatg agggaataat attccttttc caacattggt tggatatgac
    15541 ttttctcttg ttataataga ttgtaaatac tctttttctg cataatgttt ttcaattaat
    15601 aaattagtta aatatgttat agcttcataa atatcatcat gttctaactc tcttattaaa
    15661 taatcagaca tcattaatct gttcataagt ttatatgaag tgtctttttc ttttattttg
    15721 tttgtgaact ctttaagttt attctcatcc tcatcagtaa ataatggtgt tgttataaaa
    15781 attggttttt ggatattttc atactcttga aatgcaatta taaaatctat agtcttatta
    15841 aagttatctc tttttaaatc ttcctctctt aatgtttcaa taatttttat gttatcaaat
    15901 ctcttttcta tctttacttt taatagcata cttactccaa aactaaatgg acatacaata
    15961 cttgcctttt tatttgattc ccttttgctg tattttcttt ctagagaggt ttgaaagtga
    16021 agtgtaagat aaccaatttc atcttccgtt atttgttttc cttttaattc aatttctatt
    16081 gaacttgata ttactgtaaa taaaaaacaa aatttagttt ttatgttatc taaaagtgga
    16141 ttttctaagt aaacattgct tttcatttga tgcattgtaa cattcaaatg acatattaat
    16201 tgttcataaa gtaatatatc attattaaaa tttattcctg attcttctga tactaaattt
    16261 ataatttttt ttgtataact tgctaattct tcatttagat ataaagattt cttaatatta
    16321 ttactattta tttgtttgtt catacccaat attaaagatt ctataaacaa tttttcttca
    16381 taatttaata ctatggataa attagatttc aattcatctt ctatatccaa tataagcatt
    16441 tttagcttaa gctctttctt agtatttgat acttttaaag aatttcccag ccttattcgt
    16501 attatattta taagcaaaaa tagcatcaat tgcttgaatg atatatctgt aagtcttata
    16561 tccatatcct cctctatagt aagcattatg ttttttacaa ttattatatc tactatatgg
    16621 aagtactcta tttcatctat gtttaatctg tatttatcta tatatttaaa taagaatgat
    16681 actaacatta ttcttatgtc tttctcatcc ccttcaatat atataccact gcctttttta
    16741 gtcgagatat ttaaattata attatttaat gctactgata atttacttat ttcttctctg
    16801 acaatatttt tatttatgta caacttatta gataaatcat ttaaagtaac ttttttatta
    16861 ttacaaagta gtaaatttaa tatctcaact tgcttatgaa gctcactatt tactctttta
    16921 acttcacctc gcaacttctg atttaccagt attttgtctg ttccatctcc actaaattta
    16981 atcccaatat taggttttct tttgatataa gcattacaaa aagtagataa ccattcatca
    17041 atatatttgc aatcatttct tattgtcctt tctgaacact caaaatcatt ggcaatctta
    17101 ttaacattta taaactcttc ttttaatagc aatgttgtta ataaattttc ttgtctttta
    17161 ttcaaacata tcacctcttt atgactcaac ctaaatttat tatatataaa aaagagctat
    17221 acataaaggt atgctagctc tcttttaata tgatatttag ctgacctggt ttttgacccc
    17281 acactcgcct actggagggt tcgcttgggt tcgccgcact actactctct ctcgatttta
    17341 ttatcggtag gtcttacttc taagccgcac catgatcatt aacccaattt ttagtgttaa
    17401 cttacgtgga tccctttgcc tgcgactggc ttggactttc aaccacagac ctaaatatca
    17461 cttattcatt ttaatatttt aaattaaaat tgtcaatata taagattatt ccataatata
    17521 attatcattt gcttttgtaa gtagagtcaa tacaaaaacc ttattagcac ccgataactt
    17581 taaaagttta gaaatttcgt ttgttgttag accagtagta aatatatcat ctattaaaag
    17641 tatattttta ttatttatta atttaacatt gtctttaaca acaaatacat tttttaattc
    17701 ttcttttcgt tctttcttat tcaatttata tagcattctt gtatattttt ttctacttat
    17761 gcaatctaat aatggaatat ttactatttt acttaaatta aatgctattt tttgtgcctg
    17821 attaaaacct cttttattta gccttttttt atgaagtgga acaaataata tataatcaaa
    17881 ttttatgttt tctaaatata atttttcttt cattatttga gctatatact tagccataaa
    17941 ggttttttgg ttatacttca gccctagcac tatctttttt gtcacatcat tgtattcaat
    18001 acaagatatt gatttatcaa aataaaagtc tttattaaaa caataactac attcttcaat
    18061 aaattctttt tcaattgaat gacgtattat tggttttcca cacttcatgc aaccatcttg
    18121 tataaaattc atttctttaa aacaactttt acatattgaa taagtatttg ttttctttat
    18181 ggatttatca caaattatgc aacttatatt ctcaggatat ataaaatcca aacatttatt
    18241 aatcatattt ttaaaattct taataaaatt atcattgcat attttcatat ttattctatc
    18301 aacaaccctt cttgtttaaa tttatttagc ttatatccta aatttgaata cctttgatta
    18361 actctattat tttttatcat atattcaaga tatttcacat ctccaactaa tacaactaat
    18421 ttttttgctc ttgttactgc tgtatataat aaattacggc taagtaacat aggaggagcc
    18481 cacgctattg gcaatactac aactggaaat tcacttccct ggcttttatg tattgttgta
    18541 caaaaactat ggtcaatttc atctaattca tcatataggt atgatacaat tttagtctgg
    18601 tcaaataata tataaatagt tttcttatct ttatctatgt gataaacata tcctatatct
    18661 ccattataga taccttctcc actttctttt tggtcttctg tttcccattt ttttgtataa
    18721 ttgtttttta cttgcattac cttatcgcca actctaaata atcttttaga aaaactttct
    18781 tcaactttaa atttttcttt tttgttaaga tacttctgta actctatatt gagattagta
    18841 actcccaatt caccttttct cattgaagat aaaacttgta tatcttttaa tttatcaact
    18901 ttataaaatt taggtaatct ttcattaacc aatcctatta tttcatttaa aatttcttca
    18961 ttagttgatt ttcttataaa aaagaaatct ttaccttttg tatttagata aagtggttct
    19021 ccattgttta tcttatgggc atttacaatt atcatacttt cttgcgcttg tctaaatatt
    19081 tcatttaatt taactacatt tatgatatta gaattaatca tatccttaag tacatttcca
    19141 gcacctacag aaggtaattg gtcactatct ccaactaaaa ttactcttgt tcctaacttt
    19201 atagctctaa gtaaattgta cattaagata atgtctacca ttgatacctc atcaacaatt
    19261 attacatctg aatttattgg gtcttcttca tccttaaaaa aagttaaatc atcatctgtt
    19321 gaaaatccca tttctagcaa cctatgtata gtttttgctt ctttattaga agtttcactc
    19381 attctctttg ctgctcttcc cgttggagct gctaaagtca cacttttacc attattttca
    19441 aatatatcta tgatagcatt aatggtagtt gttttaccag ttcctggtcc accagttata
    19501 attaatactc cactattaac agattctttg actgctaaaa tttgtttttc tgctaactta
    19561 attttcttat cttcttctaa aacattaatc tcgttatcta tatctatttt taagtcttca
    19621 aattcatact gtgataattt tactatttga ctacatactc cattttcagc taaatagtat
    19681 ggcataaggt atataagatt ttcattgttt actttctcta tatgtatttt ttgattataa
    19741 actagcatca ttacacactc ttttattatt tctccattta gctccaataa tttttccgaa
    19801 tcttgtatta gaacatgctc tggtaaatat gtatgcccat tacttaaaga tttatttaaa
    19861 gtgtataaga ttccctgaca aattctatct ttagagtttt tatctatacc aattttactt
    19921 gctatactat cagctacttt aaatccaatt cctcttatat cttcagcaag ttggtatggg
    19981 tttttattta taacttctat tgcactgctt ttatattttt tatatatttt taggcagtaa
    20041 tttggtgtta ttccaaaagg agatagctgt attattatat ttctcaattc tctgtcttct
    20101 tcataacttt ttactatttg ttttactttt ttactcccta tgccttctac ttcttgtagt
    20161 ttttctggtg aattttgtat cacctctaac gtatctacac caaacttatc aattattctt
    20221 tttgccattt tttcaccaat accatgtatc attccagaag ataagtaaac ataaattcct
    20281 tctaaggatg atggtgttac tggcataaaa ctattcactt caaattgagt cccataaatc
    20341 ttatgattta cccactttcc ttcaacttct atgctttctc caattgccaa agtaggcata
    20401 catcctacaa ccacaatttc atcattttca tttaccaggt gagctattgt ataaccatta
    20461 tcttcgtttt taaagactat ttcacttatc attccttcta atttttccat ttttatctcc
    20521 taaaaatact aataatcgct ccatttaaat gttagtataa ttttatatat taaaactatg
    20581 ataatcgtag aaaagtttta tatttttata cttaacgtaa tttccaacta ttatacattt
    20641 tatcagaaac aaaaaaaatc tgcatccata aaatatggta ccatgaaata caggtaccat
    20701 atttaaatag ctattttatt ttagttactc tatatttaga gtataaatta aatttcataa
    20761 gtatattaca gtactaactt atttcagatt tattctcctt gtacccccca gttttagtaa
    20821 ttcttctata aagcatacat agtaaaatta aaattcccat gtaacctgta tatggatata
    20881 gtgtacctac taacttatca aatggcagta aacctcctat acaagctaat atacttacca
    20941 ctattgttat aattttaaac ttagggtggt catcttctac aaatcggttt gttactgacc
    21001 ataacaatgg tacagctgta gtgtatattc ctagtaaaag tactactgag aataaaatac
    21061 ctattattgg agatatctta tcagctaagt aaagtgctgg tatctctttt gtgtatatat
    21121 ttcctatgtc tgaaagtaat gccaaattca tcattatagc tgctgccata agtgctactc
    21181 cacctactat accaccccat acagcatctt tcttatttac tgcagaagct cccattcctg
    21241 tcaagaatgt tattactatt attacattgt aacaagtata aagtactccc gagaaatatc
    21301 catttgatgt agcagtttta atatttaaac tatcaaccat atttccagca tttgataaag
    21361 catcaatatt gctaaataaa ctaattgctc ctactaaaag tgtaaatatt attattatag
    21421 gtcctatatt acctagtatt ttagaaagcc ttgtcaaacc taatgaaaca gttattaggg
    21481 aaacgattgc cataccaatc ctacctacat atggatttaa tccatagtac tctgataatg
    21541 tagctcctgc tcctgcaatc attattacta atgttccaaa taaaaacaat ggaccaaacc
    21601 attcaaaaaa tgttccaaga tattttccac aatatacttg ataaattttt attggctcct
    21661 taagtttcaa ttctcttccc ttatctataa cttctgctcc aaaccatgaa aatagtacca
    21721 tagagattat tccacctata atacccgcat atccataaaa cgtaaaaaat tgcagtattt
    21781 cttgaccagt tgcaaatcca gaacctatta cagtagcaac ataagctcca gcaaaactta
    21841 taaccgtttt aatatttact ttgcctgaca tacactactc ccccccaatt aaaattattt
    21901 ttaaagtgta agtttattaa gattatatta gactcattat tgtgatattt caatatattt
    21961 ttaattattt aacattagtt aacacattct taaccatcct ttaacatata tcccataata
    22021 tgtataaatt tagtagagta ttatataaaa tgtattgacc agattaaata ctgtttattc
    22081 attatatgat gataaattaa catttattaa aaatatttaa ctcataataa aaaagctgtt
    22141 agaaagtaat cataaaatta cattctaaca gctttttagt taaaaattat tcacctaaag
    22201 catcttttct taactgctca accttgtcag ttctttccca aggtaaatct atatcagttc
    22261 taccaaagtg accataagca gcaacctttt tataaagagg ctttcttaaa tctaagtctc
    22321 ttattatcgc tcctggtctt aaatcaaagt gtttatttac taattcaaca agtttttctt
    22381 ctgaaacctt gcctgtccca aaagtatcta taaatataga taatggtcta gctataccta
    22441 tagcatatgc aagttctatc tcacatttgt ctgcaagacc agccgcaaca atattttttg
    22501 caacatatct agctgcgtaa gcagcagatc tatcaacttt tgtagggtct tttcctgaga
    22561 aagctcctcc accatgtcta gaatatccac cataagtatc tattattatt tttcttcctg
    22621 taagacctgt atctccttgt ggtcctccta taacgaatct tcctgttgga tttatataaa
    22681 ttttagtctc ttcatcaagt aattctgctg gtataacttc cttaataaca tgattaatta
    22741 aatcttctct tattttatca tttgatactg tttcacaatg ttgagctgat ataagaactg
    22801 tatgtactct tacagcttta cttccttcat attcaacagt aacttgagtt tttccatctg
    22861 gtcttaaata atcaactaaa cctgtttttc taacttctgt aagtcttctt gataatttat
    22921 gtgccaaaga tatcggaagt ggcattaatt ctggagtctc attacatgca aatccaaaca
    22981 ttataccttg gtctccagct ccaacttttt ctatttcttc ttctatctct tctccagtct
    23041 tactttctag gccttcatca actcccatag ctatatctcc agattgttca tcaatagaag
    23101 ttataactgc gcaagtatca caatcaaatc catattttgc tcttgtatat ccaatttctc
    23161 ttactgtttc tcttactaat ttaggaatgt ctacataagc agatgtactt atttctcctg
    23221 ctactaaaac taaaccagta gtaactgtag tttcacaagc tactctagat tgtgggtctt
    23281 tttctaataa tgcatctaat attgaatctg atatttggtc acatatctta tcaggatgtc
    23341 cttctgtaac tgattctgac gtaaataaat gtcttgccat ttttattccc ccttatttat
    23401 tcttaatttt tattataggt tttaccttat ttttaatttt agaaatttac tcaaaataaa
    23461 aaaacctctc ctttataaga agaggttaat aatcgtaatt acatactaat taactgacct
    23521 catctttcaa gacttttgtc ttgtaggaat tagcaccttt tcccatttgg gtggttgctg
    23581 ggcttcacag ggcctatccc tcagcctctc ttgataaggt aatcaagatt taagttttgt
    23641 tttactttac tagcttacta tttttttaaa attttgtcaa tatttttagt catatttttt
    23701 ctattccttt ttttgtaaga caaataaaat tttctatcta tctgaaattt aaagttaggt
    23761 ctttttaatg ctttttattc atatattttt ttgaaggggg tgttcctatt gtatttagct
    23821 aaagtaaact acagcgatga tgatgttata cagatgctaa gttcagttct aaaaactata
    23881 attaatgaaa atacgattgt tgtttgcata ggaacagaca gggctatagg tgatacttta
    23941 ggccctttag ttggaacaat acttaagaac agtaacttta aataccccgt ttacggtaca
    24001 ctagataacc ctattcacgc cttaaatata tatgagtctt tagatactat aaaaaataca
    24061 catatacaag gtaacttctt ggctatagat gcttgcttgg ggtcgcagag taacataggt
    24121 aatatacaga ttagagaagg acctatcctg cccggaaaag gtgttggtaa aaaactacct
    24181 caaataggaa attattctat agtaggaatt gtagataaaa ttgatgaaaa taataaactt
    24241 tcatttaata atatacgcct tagctttata ttagatttag ctgaaacaat agccttggct
    24301 ttattagtat ccacctaatc taaatataaa ctaaatgata aaattcttta tataaaaagg
    24361 tagatatttt agagtatctg attaaatact ccaaattatc taccttttta cttttatatt
    24421 tcaagttttt atttttctac taaagcaaac attaattcac ctttacagac aagttcttca
    24481 cctacatatg ccttagcatc tgccttagct atattttttc taagagttgt catttcaact
    24541 tccatagtta atgtatctcc tggtcttact tctctcttga ttctaacttt atctatacct
    24601 gcaaatacac ctagttttcc tttgttttcc tctacagaca tcattgccac tccacatact
    24661 tgtgctagag cttctactat tagtactcct ggcatcaaag gatattctgg aaagtgacct
    24721 tggaaaaatg gttcattaac tgttacattc tttataccaa cagctctttt cccaacttct
    24781 agctctgtaa tcttgtctac taataaaaat ggatatctat gtggtattaa tttttttatt
    24841 tcatctatat ttaacattct aaactctcct aattattttc ttttgaaaga cccaccagtt
    24901 tcaagtaagt ctattgaacc taaagctatt cctgtacctt ttgcaacaca agataatggt
    24961 tcatctgcta ttgtaacagt tattcctgtc ctctgttcta ttattttatc taatccatat
    25021 aataatgaac ctcctccagt cattattata ccgacatctc ctatatctgc tgctaactca
    25081 ggaggcgttt tttccaaaac agcatgagta gcaactacta tttgctcaac acattctcta
    25141 agtgcttcca acatttctgt tgaatttata gttatagaac taggaagtcc tgtaattaaa
    25201 tttcttcctg ttatcttcat atatttttct tcttctctct taaatgcaga acctatttca
    25261 aacttgattt tctctgctgt tctttctcct ataagaagat tgtgttgttt tttcatatat
    25321 ttaactatag catcatcaaa tctatctcca ccaaccttta tagattcact tacaacagct
    25381 cctcctagag atattacagc tatatctaca gtacctccac caatatctat aatcatatta
    25441 ccattaggct gtgatatgtc tacaccagct cctatggcag ctgctattgg ttcttctatg
    25501 atatatactt ctctagctcc agcttgtctc gtagcatctt ctactgctct tttttccact
    25561 tctgtgacac ctgttggtac acataccata attcttggca taaaaaatct tccaaatccc
    25621 tttttatcaa ctatcttatc tataaaagat tttaacatct tctcagttac atcataatct
    25681 gaaattacac catctcttaa aggtcttata gcgactatat tacctggtgt tctccctatc
    25741 atctttttag cttcttctcc cacagctaaa acagaatctg ttcttttgtc tatcgccaca
    25801 acagatggtt cttctaaaac gattccctta ccactaacat aaacaagcac atttgcagta
    25861 cctaaatcta tgcctatatc agctccagcc attttttcaa ctcctttatt tataatatta
    25921 gattatatta taagcctatt ttaatactcc tataataaag catatacaaa aatctcgctt
    25981 taatcaagag aaaataaatc ttttgagagt attattattt tgctatttat aattacttaa
    26041 tatatttctt tttataattt aattcaataa aaaaagtagg tttttaacct acattttttt
    26101 atgttccttc tcatatttta atttcgttgc taatccccct ctaatatgtc tctctgattt
    26161 atttttgtcc agcacatttt taacttcttt ggcaagggat ggatttattt ccttcaatct
    26221 ttctgtaaca tccttatgaa ttgtactttt acttactcca aatgtcttgg cggtttgtct
    26281 tactgtagtg tttttttcaa gtatatattt tgctaccact atggcccttt cctctatatg
    26341 agatctcaaa gattaccccc ctcacataat agctttaatg atatatatgt aaaaacacca
    26401 aaaaatataa caagcttaac caaatatgat taaacttgtt atactattac ttaattagac
    26461 tcattgggtc tatagatttt tctccactat acgcctctaa atgcacatgt ataccttctt
    26521 cactttctat ttgagaagta ctacctacag tacctagaga ttgtccttct gttaccttct
    26581 gttctttctt aacactaaca tttttatcta gatttgaata tactactaca atgttattgt
    26641 cactttttat tttgactgat tgtccatatt cttcatcatc aaatacatct actactattc
    26701 cgtttaatag agattttact tctttgcctt tagtacaact aatatctaca cctttatgag
    26761 tttcccaaac atcaagtgtt ttagaataac taggctcttt ttctgaatat cctcttatta
    26821 ctttatctcc aatgtaattt aattttgttg tactcgattt attttcctct tttgacttag
    26881 ctttttctaa gttttgtttt gaatccgttg cagtagggat agcatctttc ttgtcttttt
    26941 caattaaatg tatttcctct tcactatctt tattagcatt ttccataatt cctttattag
    27001 aagccaattt atctacatta ttatttgtaa accaaactcc acctactgct aataaacaaa
    27061 cacatacaaa caaagataaa taaaaaccat ctttttctaa cagctttttc ttcattttgc
    27121 acctccaaat atatttctac atttggatta ttgacgtgtt tttggcattt tatacaatta
    27181 ttagtatata tcttttattt ttgtatctgt gtagtaatgt gataatatat cataatattt
    27241 atagccctct tctgccattc cttctgctcc ccactggctc ataccaacac catgaccata
    27301 tccttttacc acgaaatcta tgtatccttc tccaaatttt atgtcaaagt ttgcagagtt
    27361 caatttaaaa acagttctaa tgtcttttcc agtcaattct ttatttccta attttatttt
    27421 ttctacagtt ccggcatcac ttcttttagt tattgaaact tgtttactta aattattcac
    27481 atctatcaca atagtgctat atgcccttct aagactttta acaaaatctg tgttacttat
    27541 tttaagtgtt gacgcaaatt taggggaata cttatcataa ggactttcca cagactttaa
    27601 ataaggatat tttgcagaaa atacttcttc actattttct gtttttcctg aagatgtaga
    27661 aaaataaaga ggaagtatcg ccttatcatt ataagttatt atttgacctt tagttcctct
    27721 aactgcttct tggattttgg aatatttatt cttaatccat tcttcaccat ttaatttttt
    27781 aagagtatca taactcttat attcttgaca atgtttataa tcagtacata ctactgcatt
    27841 tttatgttta ctagatttac catgttcctg tttatatact acatatgttc tagctgctac
    27901 agattgagct tttaaagctt ctatatcaaa ctctgaagac atctcaccag ctagtactcc
    27961 acataaatag ttctccatgt ccatcttttc ggttttccct gttatgtgat tatatacatt
    28021 gataattggt gcttttttat ttacagtttc atagttcact gtattttttt cttctttatt
    28081 acctttgtct tcaatatcac tttttttaat agttttgttt atagatactg ggctctctgg
    28141 tttttcagtt aattctacat ttttataaga aactaaagtt ataagtgatg gaactaacac
    28201 agagcaagtc acaaatccta acaaaacaac caatgggttc ttcatatact ctcctcctta
    28261 gcttttaaaa ccttgtattt aagtatatga acaaccctat taatttataa ctttcttcta
    28321 taaaccaatt attaatcttc tactatttcg atttgaccac ctagtgctgt tatcttctta
    28381 tctatatcaa cataacctct ttggatatgg tatatttctc ctatttgagt ttctccttct
    28441 gctataagac cgcataaaat aagggctgcc cctgctctta aatcagtagc gtttactgct
    28501 gcaccgtgta gctcatctac accattaaca accgcacttc ttccatctat ttttatatta
    28561 gcacccattc tattaaattc agcaacatgc ataaatctgt tttcaaaaac agtttctata
    28621 actacaccag ttccattggc tacagtaagc atagccataa attgtgcttg aacatctgta
    28681 ggaaatcctg gatgtggtaa agtttttata tctattggtt ttaatacttt tggacctaca
    28741 actcttacag aattgtccat ttctgttatt tcacaaccag cttctcttaa tttagctaca
    28801 actggtttta aatgctccat taaaacattt tcaacagtta tatcaccttt tgtcatagct
    28861 gctgcaacca tataagttgc tgcttctatt ctgtctggaa ttacattatg ttctgctcct
    28921 tttaactctt taacaccttt tatttttata gtattagtac ctgcaccttt aacatctgct
    28981 cccatttcgt tcaagaagtt tgctaaatct actatttctg gctcttctgc tgcattctct
    29041 attattgtag tcccttctgc taaagaagct gccatcatta tgttttcagt tgctccaaca
    29101 gatgggaaat ctaaatataa tttatttcct actaactttt ctgttgctgc ttcaacaaaa
    29161 ccatggtcca tttctatctt tgcacctaaa gctttaaatc cttttaaatg taaatctata
    29221 ggtcttgttc ctatagcaca tcctcctggc atagaaattt tcgtactatt aaatctagca
    29281 agcaatggtc ccataaccaa aaaagaagct ctcatctttc ttacaagctc atatggagct
    29341 tcacaagttt ttatattgct tgcatcaaca gtaagtgtat tttctttata ttcaacttct
    29401 gctcctacat gtcttaataa atctgatata acatgaacat ctcttaaatt tggtactcca
    29461 tttaatgttg attttccgtt tgctaataaa gtcgctgcta ttattggtaa aactgcattt
    29521 tttgcaccat ctattttaac actaccttta agtggattac tcttttttac tattatctta
    29581 gccatatttt ttgatctcct cataattaat aatctaattt tataattggt gtggcaatat
    29641 atatgagtgt ttttccgtca tcctcattat atttaattcc tatatttaaa ttaatttttt
    29701 cccctaaata ttctaattca taatcattta atactttact atatgctgtt atagataaaa
    29761 agtttacgtc tttaacttca tctatttgct tagcactcat attatataat attttttgta
    29821 aaatatcatc atatttgttg ttttgtaacc tttttgtgta ttctccaaca atacaagcag
    29881 acacatctac ttgagatgaa tatttattta acacattttc tacgttacta taaataacct
    29941 ctttatgttt atatactttg ttatccaata tgtcaactac tatgtaactt tctttgttat
    30001 ttttatttat tccttttata gaaatttctt tatcgctacc ttttatagta gcacataatt
    30061 gtattttatc gccagaccct ttttgattcc aatttattcc ttttttatcc aactttagat
    30121 tgttaattat ttctataaaa atattatgaa attctttatt tgataaatta tgatttataa
    30181 cagaattaat tttaacatta taaaatttaa aatttgcttc tgatatttca agagct
//
LOCUS       DAEMGG010000042        29988 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEMGG010000042 DAEMGG010000000
VERSION     DAEMGG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.042"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            825..2495
                     /locus_tag="KPK24_001726"
     CDS             825..2495
                     /locus_tag="KPK24_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG2299400.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHKMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESSKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2617..3351)
                     /gene="pflA"
                     /locus_tag="KPK24_001727"
     CDS             complement(2617..3351)
                     /gene="pflA"
                     /locus_tag="KPK24_001727"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG2299401.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3511..5742)
                     /gene="pflB"
                     /locus_tag="KPK24_001728"
     CDS             complement(3511..5742)
                     /gene="pflB"
                     /locus_tag="KPK24_001728"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG2299402.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6035..7096
                     /locus_tag="KPK24_001729"
     CDS             6035..7096
                     /locus_tag="KPK24_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG2299403.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7187..8800
                     /locus_tag="KPK24_001730"
     CDS             7187..8800
                     /locus_tag="KPK24_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2299404.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9075..11060
                     /locus_tag="KPK24_001731"
     CDS             9075..11060
                     /locus_tag="KPK24_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG2299405.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            11065..11499
                     /locus_tag="KPK24_001732"
     CDS             11065..11499
                     /locus_tag="KPK24_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBG2299406.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11528..12088
                     /gene="srlA"
                     /locus_tag="KPK24_001733"
     CDS             11528..12088
                     /gene="srlA"
                     /locus_tag="KPK24_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG2299407.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12152..12520
                     /locus_tag="KPK24_001734"
     CDS             12152..12520
                     /locus_tag="KPK24_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBG2299408.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12545..13111
                     /locus_tag="KPK24_001735"
     CDS             12545..13111
                     /locus_tag="KPK24_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG2299409.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13152..13517
                     /locus_tag="KPK24_001736"
     CDS             13152..13517
                     /locus_tag="KPK24_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG2299410.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13618..14391
                     /gene="srlD"
                     /locus_tag="KPK24_001737"
     CDS             13618..14391
                     /gene="srlD"
                     /locus_tag="KPK24_001737"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBG2299411.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14740..17094
                     /locus_tag="KPK24_001738"
     CDS             14740..17094
                     /locus_tag="KPK24_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2299412.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSI
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDMALHKVNCVSDELDMALHKVNCVSDELDMTSNKVNYVSDELDMT
                     SNKVNYVLDELDITSNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVE
                     LERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVK
                     PAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFT
                     IHNPVAWEDKKFETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNH
                     HADTLFSQHFMDKKSIAQAYINQRMAKKVGIEEKDIVILRSKKAKINVQINIDDGVGN
                     YIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            17182..17517
                     /gene="spoIIAA"
                     /locus_tag="KPK24_001739"
     CDS             17182..17517
                     /gene="spoIIAA"
                     /locus_tag="KPK24_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG2299413.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17531..17959
                     /locus_tag="KPK24_001740"
     CDS             17531..17959
                     /locus_tag="KPK24_001740"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG2299414.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17982..18746
                     /gene="sigF"
                     /locus_tag="KPK24_001741"
     CDS             17982..18746
                     /gene="sigF"
                     /locus_tag="KPK24_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG2299415.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            19026..19463
                     /gene="spoVAC"
                     /locus_tag="KPK24_001742"
     CDS             19026..19463
                     /gene="spoVAC"
                     /locus_tag="KPK24_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG2299416.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19630..20643
                     /gene="spoVAD"
                     /locus_tag="KPK24_001743"
     CDS             19630..20643
                     /gene="spoVAD"
                     /locus_tag="KPK24_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG2299417.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20657..21019
                     /gene="spoVAE"
                     /locus_tag="KPK24_001744"
     CDS             20657..21019
                     /gene="spoVAE"
                     /locus_tag="KPK24_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG2299418.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            21202..22218
                     /locus_tag="KPK24_001745"
     CDS             21202..22218
                     /locus_tag="KPK24_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2299419.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            22593..23435
                     /locus_tag="KPK24_001746"
     CDS             22593..23435
                     /locus_tag="KPK24_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG2299420.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            23450..23914
                     /locus_tag="KPK24_001747"
     CDS             23450..23914
                     /locus_tag="KPK24_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299421.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            23901..25073
                     /locus_tag="KPK24_001748"
     CDS             23901..25073
                     /locus_tag="KPK24_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2299422.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            25117..26052
                     /locus_tag="KPK24_001749"
     CDS             25117..26052
                     /locus_tag="KPK24_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG2299423.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(26326..29019)
                     /locus_tag="KPK24_001750"
     CDS             complement(26326..29019)
                     /locus_tag="KPK24_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG2299424.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKDDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            29233..29931
                     /gene="yunB"
                     /locus_tag="KPK24_001751"
     CDS             29233..29931
                     /gene="yunB"
                     /locus_tag="KPK24_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG2299425.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
BASE COUNT        11375 a         3578 c         5182 g         9853 t
ORIGIN      
        1 taattaaaat aaaaaatatt attttatatt tataaacatt gacattctaa ttttgtagtg
       61 taatcgtatt ttttattaat tcttacaaaa tcaatgaaaa taagtatatc cattttaaag
      121 tacataaatt taaaaatcca gaaaaatata cttaaattat gtaagtaaaa gaggggttga
      181 atcagttgaa aatgtaatat tagcaaatag tacatcaatc ctatataccc tgtaagcaac
      241 tctattttca tgtactaaaa atgtacctat tttattaact gaaaagaata aattaaatgt
      301 aagtacataa gaagatagaa aaaattaaaa acaaaaaata tttatataaa taaatataaa
      361 aaatgatata atccctatta aaaaatttat agatgatgaa gaaatatcaa aatcttatat
      421 tataggaaga gacacttctt tgccaaaatc tatagaaaaa caataccaaa tccaaagaga
      481 ataggcggag aaaaaaataa aataaattca agagtaatag aaaagtttta tagaataaag
      541 aactgataac tctttatata acgaaaaata gaattttaag agaaaatgat ttaataactg
      601 atttaacatg gtgttttagc aggtaaaaat aaatcaccag taatgattat agaaaaatta
      661 ctttatgaat ctcaaaaaac ttagttaaga gtaagagatt tgataagata actaaagttg
      721 gggaaatgtg tttgatgaga caaaaaatta gtaaaatagt ataatctaaa taaggatatt
      781 ttgttgatta aactacacta atagtaatat tgtaggggga aaagatgaga gaagaatcga
      841 actctttaag tttagaccat atagaaattt tctttgaact tttatctcaa agtacagaag
      901 attatatatt tttttgggat ataaatagga ataaatttaa aatttcttca gcaatttttg
      961 atgaatttaa cttaagtaaa gagatagaat ctgatttggt caattgttgg agtaaaattg
     1021 tttatccaga tgatgttcaa atatggaaag atgatataca agaattacta catggaaaaa
     1081 aaggtgagca caatttagaa tataggttga ttaataaata taaagaaata gtatggattt
     1141 cttgtagagg taaagtttat gtgtcggatg acccaaaaac tatatttcta gtcgggagaa
     1201 taaagaatat tggtgaaaaa aataaatttg attctatcac aggaacgtgg aatagagaac
     1261 aatttgaaca taaaatgaat tatcttataa aagagaaaat ttataaaaat ggtgctatgt
     1321 ttattatgga tattgataat tttaaaaata taaatgaaaa atatggacat tcttatggtg
     1381 acaaagtgtt gcgtgcaatt gctactgaag tactggaata tcttccaaaa gatgtaagat
     1441 tgtataggtt agatggagat gaatttgcat ttttttaccc tatgtgtact aaagaaacta
     1501 tagagaaaat atatgaaaag atacagatgt atacaaatac tcagcatgaa attgaatcaa
     1561 gtaagtacta ttgcacagtg actgcaggcg ttgctatgta tccagaagat ggagataatt
     1621 atttagattt atttaaacat gctgatattg cattggatat agcaaaaatt agtggtaaaa
     1681 atagaattaa gtttttttca caagagctct atgaaaataa actaaaggta atttcaatgc
     1741 aacaaaaact aagagagtgc gttgaaaata attttaatga ctttgagctg ttttttcaac
     1801 cccaggtaaa tgctgttaca aaagaggtaa taggggcaga ggtactttta agatggcatt
     1861 cgagtactta tggagaagta tctcctgttg aatttatacc aatactagaa cagagtaatt
     1921 taattatacc agttgggaag tggattataa aagaagctgt aaaacagtgt aaagaatggc
     1981 ataaaataaa tccagacttt aaaatatctg taaatgtatc gtatattcag ttaaaggaag
     2041 atttttttag agattttata gtagaatgtt tagtagagta tcaattaaga cctgaatttt
     2101 taattttgga attaacagag aattgttgga taccagatat aaatttacta aatgataaat
     2161 ttattagttt aaagggtata ggagtatata ttgcaataga tgactttggt acaggatatt
     2221 catctttaaa ttaccttaaa gaactgtctg ttaatataat aaaaattgaa agaagttttg
     2281 ttaagaatat tacatataat agttatgaat atacttttct tgaatatatt attaaattag
     2341 ctcatattat aaatttaaaa gtttgtgttg agggaataga gtcttatgaa gagtatgata
     2401 tagtaaaaag cttaggggta gatattattc agggtttttt atttggaaga cctgtaagtg
     2461 cctctgaatt ttataaattt cagctatcaa agtgatgtat ataaaatgag atatatagta
     2521 tataaaaata gggcaaagtg gaagttttga agtttattaa aaatttacac tttaccctat
     2581 tttgttatgt cttctataaa ggattaatat actacactaa ctattatata ctttcactcc
     2641 aaatttagag attatttcac tagctttttg gacatcttcc ttagtggcat caggaacatc
     2701 ttttagttca taatcgatac ctaaactttc ccatttgtgt attcccatag aatggtatgg
     2761 tagcaattca aatctttcta catttttcaa tgtagctaca aattgaccta gtttttctaa
     2821 attatctata tcatcagtaa tgccaggaac taaaacatgt cttatccata caggaatatt
     2881 tcttgattct aagtgtctag caagttttaa tgccttatcc ataccaacac cagtcaaatc
     2941 aatagatttt tcctcaacca tatgttttaa atcaagtaaa accaaatcag tattatctaa
     3001 aactgggtct atagtttcta tatctacaaa tccagatgta tccaagcaag tgtgtatatc
     3061 attttctttt gcttttttaa ataattcact taaaaattct ggttgaagag tggattcacc
     3121 accagaggca gttatacctc caccagaaaa cttcataaaa gaagtatact taagagcatc
     3181 cgttattatt tcatcaactg tgtattcttt tcctgattta atatcccatg tatccctgtt
     3241 atgacagtat ttgcatctaa gaggacaccc ctgaaaaaat aatatatatc ttattcctgg
     3301 tccatctaca gtaccaaacg tttctataga atgtatcttt cccttaatca tagagttcac
     3361 ctcacaatat attttaataa ccttatcaat agttataata tttctctaaa aaaggccatc
     3421 ttataccaga aataattcaa atataagata gccttttatt tttatttttt acacagtagt
     3481 aatcttagtt aaagtataaa aatttaaaga ctaagccatt ttaccgtgga atgttctgtt
     3541 tataacatct aattgttgct ctttagttaa ttttatgaag ttaactgcat atccagatac
     3601 tcttatagtt aattgtggat attcttctgg atgttccata gcatcttcta aagtagctct
     3661 atcaaataca tttacattta agtgatgagc attttgagcg aagtatccat ccatcattgc
     3721 agaaagattg tttattcttt cagtcatatc ttttcctaaa gcaccaggta ctatagagaa
     3781 agtattagat ataccatctt gagcatgttc atatggtaat ttagcaactg aagataatga
     3841 agctaaagca ccattagtat ctctaccatg cattgggtta gcaccaggag caaatggagc
     3901 accagctctt ctaccatctg gtgtattacc agttttctta ccataaacaa catttgaagt
     3961 tattgtaagt atagattgag ttggataaga atttctataa gttttattct ttcttatttt
     4021 attcataaag ctttcaacta actctacagc tatactatca actctatcat cattatttcc
     4081 gtattttgga taatctcctt ctatttcaaa atcaacagct attccttgtt catttctaat
     4141 agttttaacc ttagcatgtt ttatagcaga taatgagtct gcacaaactg ataatccagc
     4201 cataccacaa gccatagttc taaatatatc tctatcatgt aatgccattt ctaatgcttc
     4261 ataagaatat ttatcatgca tataatgtat tacatttaaa gtatttacat ataatgttgc
     4321 taaccaatca gtgaatggtt caaatttact cataacttca tcataatcta agtactcaga
     4381 agttataggt tcaaatcttg gtccaacttg aacaccagat ttctcatcta caccaccatt
     4441 tatagcgtat aataaagttt tagctaagtt aactctagct ccaaagaact gcatttgttt
     4501 accaattctc attgcagata cacaacaagc tataccatag tcatctcccc agtatgctct
     4561 cattaaatca tcattttcat actgtactga acttgtatct atagatactt tagaacagaa
     4621 atctttgaat ccttgaggta atttagttga ccatagaact gttaagtttg gttctggaga
     4681 aggtcctaat gtatatagag tatttaacat tctaaatgag tttttagtaa ctaatgttct
     4741 accatctatt cccatacctc ctatacactc agttacccaa gttgggtctc cagagaatag
     4801 gttattgtaa tcaggagttc ttaggaactt aaccattctt aatttcataa caaaatggtc
     4861 catatactct tgtaattctt cttctgttac gattccagct tttaaatctc tttcaaagta
     4921 tatatctaag aaagtagatg ttctacctaa agacattgca gcaccatttt ggtctttaac
     4981 agcacctaaa taaccaaagt ataaccattg tacagcttct tttgagttag ttgctggttt
     5041 tgagatatca aatccataag attcagccat ttttttaagt tcatttaaag ctgatatttg
     5101 ctcagttatt tcttctctta atctgataac ttcctcatcc atgcaagaaa cttctaatga
     5161 ttttttttgc tcatttttat cttctattaa agcatcaaca ccgtatagag caactcttct
     5221 gtagtcacca attattctac ctctaccata agcatctgga agacctgtta ttataccaga
     5281 ttttctagca gctctcattt ctggagtata aacatcaaaa actccttggt tgtgtgtttt
     5341 tctgtattta gtaaatatat cttttatttc tgggtctaat tcatatccaa aagcttcgca
     5401 tgaattttca accatcttta taccaccata tggcatgaca gctcttttta aaggggcatc
     5461 cgtttgtaac ccaactattg tttctaattc tttatctaaa tatcctggag catatgcagc
     5521 tattccagaa acagtttttg tatcaacatc taaagtacca ccattttctc tttctttttt
     5581 aaataggacc attgcttcgt cccataattt tttagtattt tcagtggcac cagctaagaa
     5641 agagtcattt ccctcatatg gagaatagtt taattgtata aactctctaa catttatttc
     5701 tttagtccat cttcctgttt taaatccttg ccatgcattc attaagtatt tcctccttag
     5761 atttaaatga taaataatac tattaaaatt aatttttata tacttaattt attttatata
     5821 tttttttaaa aatatatttt ctaatataat aatagcatat tattcgtatc ctgtatacat
     5881 aaaaaataaa aaattgtcgc tttaaagtat aaaacagatt atatgttaat aatgtggtga
     5941 aattataccc atatataaat aaaataaaac aaataaatag ctaattaagg agaaaatatt
     6001 ttttattttt tgtttaggat aaataaattt tattatggaa aaaagaaaaa gcatacatct
     6061 tcacaaactg aatatttatt taataggggg aggtgataga ttgtttatca cagtggtatt
     6121 atttttagta ggtttcctat taattactaa aggcgcagat atctttataa attgtactgt
     6181 agagataggt aaaaaaacga atatatcaga aattatactt ggagctacta tagttagttt
     6241 tgcaacaaca ttacctgagt ttacagtgtc tctacttgct tctattgatg gtcatactac
     6301 tatgagttta ggaaatgcag ttggttctat aatatgtaat acagggttag cattagggtt
     6361 ggttgttttt ataagtccat ttaatgtaga taaaaaaatg tttttttcta agtctttact
     6421 attaatagta tccgttatag ttttaatttt attgagttta gatggagtta taacaagagg
     6481 tgattcatta ttacttataa taatattaat cttttatatg gttaataatt atagaagtgt
     6541 cgttggaaaa tctgatacta aaaatagaaa tattaaaaat aacactatta aagacatatc
     6601 tactaaaaaa gggaaaaatt atagaggttt ttccgtatta gaaattgtaa aaatactatt
     6661 actttttgct acaggattga taatgatgat aataggttca caaatcctta tagagagtgg
     6721 agtaataata gctagttttt taaatatacc tcaaggtata gtaagtttaa ctataatagc
     6781 acttggaact tctctgccag agatagtttc gtcgattact gctataagaa aaaatcatca
     6841 tgaaatatca gtaggaaata ttttgggtgc aaacatattg aatatagtat cagtaatagc
     6901 tgtatcagca ataccaaata acataccaat actatctcaa aatagacaat tggatatacc
     6961 attcatgata ttattattat taatagtaat aataccaaca cttaaatcta ataaacttag
     7021 tagaatacaa ggtatattga tgttattcac atattttctg tacatatcaa ttttgtattt
     7081 catgtatatt atttaactat actgtttaaa atattaattg cagaatatcc taaaaaagta
     7141 tataatggat aggttaagat taattgataa cggagaggaa taatatatga atataacaaa
     7201 gtttgaagat ttaccaataa gtgagggaat aaaaaaagct atagctgaaa tggggtttga
     7261 agaaccatct cctatacaag cacagtctat acctgcgatt ttgtcaggta aagacgttat
     7321 aggacaagct caaactggta cgggaaaaac agctgctttt agtatcccta tactagaaac
     7381 aatagaccca aataatagaa gtttacaagc tgtagtgctt tgtccaacaa gagaactggc
     7441 aatacaagta tcaacagaaa taagaaagtt agctaagtat tcgcacggaa taaagacatt
     7501 acctatatat ggtggtcagc caatagatag acaaataaaa tctctaaaga gtggtgtgca
     7561 agttgtaata ggtacaccag gacgtacaat agaccatatc aatcgtaaaa cattaaagat
     7621 ggataatgta aaaatgatta tattagatga agcagatgaa atgctagata tgggatttag
     7681 agaagatata gaaatgattt taagtaagat tccagaagaa agacaaacaa ctttcttttc
     7741 agctactatg ccaagaggaa ttttggaatt aactaagaga tatcaaaaag acccagagca
     7801 tataaaagtt gttagaaaag aacttacagt ttcaaataca aagcaatact atatagaaac
     7861 taggtcatca aataaattag aagtattatg tagattagta gatgtatatg accctaaact
     7921 atctgttgtt ttctgtaata ctaagagaaa agcagacgaa ttggtaggag atttacaagc
     7981 aagaggatat tttgcagatg cattacatgg tgacttaaaa cagactcaaa gagatatagt
     8041 aatggataag tttagaaatg gtacaataga tatacttgtt gctactgatg ttgcagctag
     8101 aggtatagat gttgatgatg ttgaatgtgt attcaactat gacttaccac aagatgaaga
     8161 atattacgtt catagaattg gtagaactgg tcgtgctggt agagaaggta tgtcatttac
     8221 ttttgtattt ggaaaagaaa tgagaaagat gaaagatata gaaagatata ctaaatctaa
     8281 actaataaag cataatatac caacaataac tgatgttgaa gagaaaaaag taggtacttt
     8341 ctttgcacaa gttaaacaaa ctatagaaga aggtcactta actaaacaat tacaatggtt
     8401 agaaggtttc tgtaatgatg aagattatgc aatggttgat atagctgctg cacttgtaaa
     8461 actatctcta ggtgaagaaa tgaaagaaga aataatagaa gaaaaaccta gaagagagcg
     8521 tggagataga aaaggtggta ctggagctaa agatggtatg attagattat ttatcaatat
     8581 tggtagaaat caaagagtac aagcaaaaga tatagttggt gccatagctg gagaagttgg
     8641 aataccaggt aaagtagtag gtactataga tatatatgat aagtatactt ttgttgaaat
     8701 acctaaaaaa gatgcaaaaa cagtaattga aaagatgaaa gatattaaga taaaaggtaa
     8761 taagattaat atagaaaaag caaataagaa gaaaaaataa cgtatagtat acaaaactat
     8821 aattagaaaa tatataaaat aatatattgg atatttaaaa agcctgatgt gttttcaaaa
     8881 cagatagtag ataatctgta agagttaaca gcataaggct tttcttgttt tatatgatgt
     8941 tataattttc cagattgttg tgaaaaatgt atattagttc ctttttttca aatctgattt
     9001 gaaaaagaga atattaatta tctatatagt ttagattata ataaaaaatg taagataaca
     9061 aaggggagaa aaagatgtca atgacttata tggaccctag ggtttttttg attttagaaa
     9121 tggtgttaga agaagacaca aatactctag aacaattttc aaatgttttg ggtgttagta
     9181 ctaggactat cagaaactat ataaaccaga ttaatcgaga attaaaaggt ggtattgcta
     9241 aaatagttaa aaatgaaaaa ggtacatata gtataagaat tgaggatgaa agtaaattaa
     9301 ttgagattgt aaattttaat agaggaaagc tatcaaattt tataaattta aattcacctg
     9361 atgaaagaat aaattatgtt cttgatattt taactatgac caacaatcca attaccatag
     9421 atgatttggc tgaagagata tgtataggaa gaacaacatt aataaaggat ttaaaaaaag
     9481 cagacagaat tttagctgaa tatgatttag aattaaaaag gaagccaaat ataggtatga
     9541 ttttagttgg tgaagaaatg gatataagat tattaatcct tgatagattg tatgaaaatt
     9601 atattgatgt cttggaaaac gttaatcaga taaatttagt taaagattat gatattgaat
     9661 gtcttagaga tgaattaaaa aagttattta aaaaagaaga actctacata acagaacagg
     9721 tcttgagaga tgttgaaaga tatataatca tgtctgtctt aagaaatcta aatgggtaca
     9781 aatttgaaaa gatagataag agatttgaag tgataagatg ttcagatgaa tatactttag
     9841 gattaaagct caaagcttta ttggaaaaga tatttaatat agttttaaat gaaaaagaaa
     9901 ccatattttt gacaatgcca ttaataggta gaaaagctcc tagtacaaaa ttagcactta
     9961 gcagtataaa gataaatgat agcgtaaagg aagttgtaga tgaagtaact agttttttat
    10021 tagagacctc agggattgat tttaaagaag ataaaaagct tatagaaaat ttggaatatc
    10081 acttgtattt tgcacttaat agaatgagat tcaacataag ggtaaaaaat cctcttttac
    10141 aagaaataaa ggaaagatat acatttccat atagtgttgc aaagatagct gcaatgataa
    10201 ttgaggaaaa gtttgacttg aaaatatgtg accacgaaat aggatatata gcattacatt
    10261 ttggaagtta ttttgaaaaa aattcaagaa aagttctaaa tgtaaatagg gtagctgttg
    10321 tttgtggaac aggtcttggg actgcacaac ttattagaat aaagatagga aaaatacttg
    10381 gagaaagtgt agaagtaaat actttttctg atatagaagc taaagctaat ttagatttat
    10441 taaagtatta tgatttagta tttacaacag tagatttcaa aacagactta gatgttccaa
    10501 tttttagagt aaatgcgtta tttaatgaag aaagtataaa gaaagagata gaaaatgcaa
    10561 tacttttgaa aaatagtata aatcttaagt ctataaaaaa gattgaaaaa aatatccctt
    10621 ttataggtaa attaattgaa gaggataagt tctttatatt gaataagagt acgtttatgg
    10681 aaaatttaga agatatgtta gaaaaattag ttcataaagg aattattgat atagagttca
    10741 aaaaaaaggt attaaaaaga gaaaagaaat cttcaacagc attagataat tatatagcat
    10801 tgcctcactc agttaatact aatggggaaa atctttatct ggcatttgga atattagaaa
    10861 aaccagtgat ttgggataca aaggaaatta gaataataat attgatgata attccagata
    10921 agaatgtaga tagtacagac ctgataataa aaagttatga agaagtactt gaagtaggga
    10981 gagataaaaa gatggtagaa gaacttgcta aggcaaaatc atttgatgaa ttttataaaa
    11041 taattaccaa gaggaggtaa taatatggac ccagtaattt ttattatagc atcacttatt
    11101 acagtgtatt tattaaatat ttttcttgga tacttacagc ttaaagattt taataaaaat
    11161 tatattgaac taaaaagaaa aggaagagtg gcaattggca gaaaaaaagg aagaatttca
    11221 tcgggtacaa ttgtgttgat acttataaat gatgatggtg tgattgtaga gactagaaaa
    11281 atgcaaggtg ttacagtatt ggcaagagta aagtcatttg aaggtttggt aggaaagagc
    11341 ttagagtata tagaaaaatc agatttaact gtttacaata aactacttaa aaaagcaata
    11401 ctagatgcag tcaaacagta cataacattt gagaaaaata aatgtgaaga tacaaataat
    11461 ctgagtactg atgaagagtg taggatggaa gtagtttaaa aaaatatatt tataaggagg
    11521 agttaatatg acgcaatttt ttcaagtatt agcaagtggt gcagatagtt ttatgaattt
    11581 atttagggca ggtgggcaac aatttgtagg ttttgtaaca gatatagtac cacttttagt
    11641 agcattatta gttacaatga atgcaattat caactttatt ggtacagata gagtcgagag
    11701 acttgcaaaa aaatgttctg caaatatatt tactagatat ttgatattac cagtccttgg
    11761 tacatttgta tttgcaaacc caatgacact atcattagga agattcttgc cagaaaagtt
    11821 taaacctagt tactttgcag cagcaagctt ttcatgccat acaatgaatg gattattccc
    11881 acatatcaat ccaggtgaac tatttatata tctaggaatt gcaaatggta taacaacttt
    11941 gggattctca acagcagatt tagcagttag atatttgtta gtgggtatag ttgcaaactt
    12001 tattaaaggt gtagttactg actatacaac aaaatttgta gagaaacaac aaggaatagc
    12061 attaaattca gatataaaga ttggataatt actttaaggt ataaataaat ttttcaaata
    12121 aaaagatagt gtatagagga ggataaatta tatggcattt aacaaagtta aaatagtaaa
    12181 aggttctgga ggatggggtg gaccattgat aatagaacca acagaaaaga agaataaggt
    12241 agtatatgtg actgggggag caaagccaga aactgcagta aaaatagctg aacttacagg
    12301 atgtgagtta atagatggat tttcacaggg tgttaaagat gatgaaatcg catgtgtaat
    12361 aataaattgt ggtggaacat tgagatgtgg tatatatcct caaaagaaaa taccgactat
    12421 aaatataatg aaaacaggaa aaagtggacc attagcaatg tttataaaag aagatatata
    12481 tgtttcaggg gtaaaaccaa caaatgtaga gcagttataa ttgaggtagt aggagggaag
    12541 aaatatggct tatgatacta aaaagaagat aagtgagcag agtcaaggaa ttgttgcaaa
    12601 aataggtata gcactaggaa atgtgacaag tactcttttt caagctggta gggatactat
    12661 agatacatta atacatacta tactgccttt tatggctttc gttgcagtcc taataggtat
    12721 aataaatcaa tcaggtatag gaaattgggt agccaattta cttactccat taatcggaaa
    12781 tgttggaggt ttagttgtct tatcaataat atgttcattt ccgttgttgt ctccgtttct
    12841 tggaccagga gctgttatag ctcagatagt gggggtattg gttggtacag aaataggaaa
    12901 aggaaatatt ccaccaagtt tagcattacc agcattgttt gcaataaact cacaagcagc
    12961 ttgtgacttt ataccagtag gtcttggtct tgcagaagct gaacctgata cagttcaagt
    13021 aggagttccg tcagttttat attctagatt tttaacaggt gcaccaactg ttctattagc
    13081 atggttagca agctttgggt tatataaata gaagaatact ttataaataa ttagagagag
    13141 gttcagttga tatggaatta atatacagta ctcaaataaa taaaatagga gataatgcaa
    13201 atgaattttt acaacataat atgtttgtaa ctttcaaaga taatgctcct caagagttag
    13261 aaaattactg ttatatacat tctgaaaata atttagtaaa agaaatactt acggatgatg
    13321 tgctatcaat aaataatgtt gattacaaaa ttacttctgt tggtgagtta gtaaaccaaa
    13381 atctttcaga attaggtcat ataacattta agtttacagg tgaaaaagat ggagcaattg
    13441 gaggaacctt atatcttgaa gaaaaagaaa tagcacctat tggcgtaggt actatactaa
    13501 aaataattag aaaataaaat ttaaaacgaa tctaatcata ttataagtaa attatagata
    13561 aaagtcaatt taactaatca agttaacttt taatgtgata aaaaagggga aatgaaaatg
    13621 agaaaagtgg ctttagtcgt tggaggagga agaagtttag gtgaatattt atctaaacat
    13681 ttagctaaag taggttatga tgttgctatt gcagatttaa atgaagacaa cgcaaaaaaa
    13741 gtagctgaag aaataaaaac tgaatttggt tgtaaatctg taggatatag ttgtaatgcc
    13801 atagatgaaa gacaggttat aagtactgtc aaagctattg aaaatgattt tgatagaata
    13861 gatttattag tgtacaatgc aggagtggct gtaagtaaaa aaataactga ttttggatac
    13921 aaagatttta aatttattgt agacgtaaat ttaaatgggt actttctatt tgcaagagaa
    13981 gtatctaaag tcatgatagc aaaaggtatt gagggtagaa taatacagat aaattcaaaa
    14041 tcaggtagag tgggaagcaa gcataattca ggatattcag caggtaaatt tgctggagta
    14101 ggacttactc aaagtttagc gctagatttg gcagaacata agataactgt aaatgcactg
    14161 atgctcggta atttacttga ttctgatatg tttgaaagtt taattcctca atatgcagta
    14221 aaattgggaa tacctgaatc agaagtcaag caagtttata tagataaagt acctttaaaa
    14281 agagggtgta gatttataga tgttgcaaat atgcttacat tttatgcttc aaaggaagct
    14341 gaatactgca ctggtcaatc tataaatatt actggtggac aagttatgta aggtttttat
    14401 ggagtgtcta aagatgaact ctttagttca tgaggcactt ttttgtataa aattaaatat
    14461 attttcagca tttaaacaat gaaaaagagg tttatcttta ttttgagata gcctcatttt
    14521 tgtaattaaa aaacatagtg tttaagtaaa catataagat tgttagtttt ttaaaagaga
    14581 attttttaat aatattatat ttatgaagta tttttaaact taatttgata aaatatagct
    14641 atgttttata tactgtagtt ttgaacatag aaagtttaat aaattatagt atatatgtta
    14701 atttttaata aggtaagaaa gtagaaagaa ggatatttta tggatataat aaaaaaatta
    14761 agtcatgggt gtacattaga ttgtcatgac tgttgtaaat tcaatgttta tactaaagga
    14821 aataatgtag taaaaattga aggagataaa aatcatcctt atacaaaagg ttttatatgc
    14881 aaaaaaggga tggcccattt agatagatta aatcataaag atagaataaa gactcctatg
    14941 cttaaagttg atggagtatg gaaagagatt tcttttgata aagctataga aataatggca
    15001 gaaaagctta catactataa agaaaagtat acatcaaagt ctatcatgca ttatgaccaa
    15061 tatggaagtg ggtctgtgct aaaatacata ggagatatat tttttaattt ttatggaggt
    15121 gttagcagac ataagggggg tccctgttgg agtgcaggga tgcatgctca aaaatatgat
    15181 tttggagtag ctaagagtca tgctatagaa gatatgttaa atagtaaaag tatatttgtt
    15241 tggggaaaaa atccagcata tactacaatt catactatgc aaataattaa aaaagctaaa
    15301 gaaaaaggga taaagatagt agtgattgac cccatatata caaaaacagc tcaaatagca
    15361 gataagtatg tacaagtaaa tcctggaaca gatggagctt tagctatagc tatggcaaaa
    15421 attatagtgg aagataaatt atatgatgaa gaatatataa actcgtatgt tattggtttt
    15481 gaagaatata aaaaatattt atcttcattg gaattaagtt ttttaattga tgaatgtggt
    15541 gttaaagaga atgatataag agagttagtg gatttatata ctaacaaata ttcaagtata
    15601 aatgttggat atgggcttca aaaatataaa aatggtggaa acacaataag agcaatagat
    15661 gctttaggtg ctataactgg tcaaatagga tttagtggtg gaggggtaaa ctatgctaat
    15721 aaagtttatc cgagtgttat aaattctgac ccttacaata gccaatcata tggtgaagat
    15781 agggagtttt atgtaagtaa tatcagtaaa tttatagaag aatctttaaa aaatacatca
    15841 aataaagtaa attatgcatc agatgaactt gatatggcat tacataaagt aaattacata
    15901 tcagatgaac ttgatacaac atcaaataaa gtaaattatg tatcagatga acttgatatg
    15961 gcattacata aagtaaattg tgtatcagat gaacttgata tggcattaca taaagtaaat
    16021 tgtgtatcag atgaacttga tatgacatca aataaagtaa attatgtatc agatgaactt
    16081 gatatgacat caaataaagt aaattatgta ttagatgaac ttgatataac atcaaataaa
    16141 acagattata tatcaaatga attgtataat ttatctaata agtcaatcaa agataatatt
    16201 cctataaaga tggctgtgat aactaaaagt aatatgctga atcaactgcc agatttagta
    16261 gaattggaga gggtgttttc aaaaattgaa tttaaagtat gttttgatat gtttataact
    16321 gatacagcta ctttatgtga catgtttata ccgtgtacaa atacactgga gagtgaagat
    16381 ataatttata gctcaatgac caatccatat ataacctata atgaaagagc tgtaaagcca
    16441 gcacataaat tgatggatga atattacttc tttatggagc tagctaaaaa gatgggatta
    16501 aatgattatc cttttgtaga aaagagaact tatttagaaa aagtgattga accacttaag
    16561 cgttttgaca aaaatctgga tatagaaaaa cttaaaaaca attactttac tatacataac
    16621 cctgttgctt gggaagataa gaagtttgag actccatctg gaaaatacga gctttattca
    16681 gaaagtataa aaaacttggg aataagtcca acacctgtat atataagcaa taaatataaa
    16741 gaaatagaag ataaaaatat ttcttttaga ttattaacta atcatcatgc agatacttta
    16801 tttagtcagc attttatgga caaaaaatct atagctcaag catatataaa tcagagaatg
    16861 gctaaaaaag taggtattga ggagaaagat attgtgatat tgaggtcaaa aaaagcaaaa
    16921 ataaatgtac aaataaatat agatgatgga gtaggtaatt atatagtaaa aatgtatgtt
    16981 ggttggtgga agaaacatgg aaatcctaat agtttaacag acactggtat atcagatttt
    17041 gggggtcagg taacatataa tgaaagtatg gtagaaataa tacgacaaaa ctagttaaat
    17101 ttcattgaag gaataaaaat ataattatag aattgattaa aagttactat ttttacaaaa
    17161 ctattgaagg agggattttt gatggtaaat tattccttag aacataagaa tctatacata
    17221 gaatttatgt gctcagaact agaccaccat gtagccaatg aaataaggga agaaatagat
    17281 aacttattaa gtgtaaatca agtaaaaaat gttgtattta attttgaaaa tattaatttt
    17341 atggattctt caggtatagg cgtcatcata ggtagatata aaaagatttc taatgaaggg
    17401 ggacgagttt cagtaattaa tataagttca cgagttaaga agatttttga tttatctggg
    17461 ttaaataaaa ttattggcat ttatgatacg tatgaagaag ctctaagttc tttgtaggga
    17521 ggagtatgtg atgaataata ttatggaagt taaattttcg gcaatatctg aaaatgaaag
    17581 tttagcaaga gttatagtag catcctttgc agctaagtta gacccaacag tagatgagtt
    17641 agtagatata aaaactgctg tctctgaggc tgttacgaat gctataatac atggatatga
    17701 agaagacagt tctaagtttg ttttcttaag atgtgagata gaaggaaata ctataaagat
    17761 tatagttgaa gatgaagggt atggaataga aaatgtggag aaggcaatgg aaccacttta
    17821 tacatcaaaa ccagaacttg atagatcagg aatgggattc acagtaatga aaagctttat
    17881 ggacgatgta gaggtaagtt ctgtaaaaga taatgggact agaattgaaa tgactaaaaa
    17941 aataaatgtg cctaaataaa gtccaaaagt gaggttttat tatggaagta actgttgcca
    18001 gagaagaaaa aaaacctctt cttagtcatg aagagactct agaacttatt gaaaaagttc
    18061 aaaatggaga tgaagaggca aaagaaatat taatatcaag caatttaggt ctagttagga
    18121 gcgttgtatc tagattttta aatataggat atgatagaga agatttattt cagttaggtt
    18181 ctataggctt aataaaatca atatataaat ttgacccaaa atttaatgta aaattctcta
    18241 cttatgcagt accaatgatt ttaggtgaaa taaagagata cttaagagat gatggtatga
    18301 taaaagtatc aaggtcgtta aagcaaatag ctgtaaaggc aaagatggaa agtgaagctt
    18361 taactaaaaa attaggtaga gagccgagta tagaagaatt agctaaagca atagatgtag
    18421 aaaaagaaga tttagtaatg gctatggaag ctaatttcaa tgttgagtat ttgcaaggag
    18481 ttattcatga agaggaaggt tctccaatat gccttattga taaaataagc atgaaaggag
    18541 aaagtgaaga agaaaaagta gtggataaca tattgttaaa agacatactt ggaagactgg
    18601 ataagagaga gcgtcaaata atagttctca gatactttga agacatgact caaagtgaaa
    18661 taggagagat gctcaatata tcacaagttc aagtatctag gatagagaag aaagtattgt
    18721 ctaagttaaa agaatatata tcgtgataat acttatgata tgtagaataa caaaatataa
    18781 taaatatatt actaaaataa aatgtaataa ctttttatgt taagttttta aattattaaa
    18841 agatattaaa ataataaaaa gcattaaaaa agtaaaaata taaaaacaag gtgtctttat
    18901 ggaaaaatag attttttctt tattaggcat cttttttatt ttttgaccaa actttagata
    18961 gtcaaaataa ataataggtt tattttttta aaattaacaa ataatacttt agagaggtgt
    19021 ttagtatgga taaaaattat aaaaaatatg tagaccaaat aagcccaaaa cctacatatt
    19081 taaaaaacta tacacttgca tttattgtag gtggaattat ttgtatgata gggcaagcaa
    19141 taaatgattt gtatatgaaa gttggaggtc ttgataaact aggtgctagt tctgcaacat
    19201 caataacatt aatatttata ggggcatttt taactggatt aggagtttac gacctaatag
    19261 gaaaaagagc tggagctggt tctataattc caataacagg ttttgctaac tcaatagtat
    19321 ctccagcaat ggagtataaa agagaaggct atgtattagg tgtaggtgca aacttattta
    19381 aaattgcagg accagtttta gtttatggta taggttcttc tattttatgt ggaattattt
    19441 attatatttt taagatgttt tagatgattt ttatttaatt taaattttat taattatgaa
    19501 gaaggtataa aatctgaaaa tagctactaa agtaactaat tgaaaaataa ttgtgcagat
    19561 ttaattaata tttttaatta atattaaaaa tttgaatagt aaaatgcaaa attaaaaagg
    19621 tgtgaaaata tgaaaaataa aagaattgga aaaagaacag tcaagttaga aaataaacca
    19681 accataatat caactggaac tatagtaggt cctaaggaag gtgaaggacc actaaaggac
    19741 tattttgata tgattatgac tgacgattta tatggtgaaa agacttggga attagctgaa
    19801 agtaaaatgg tagagactgc atctcaacaa gctatccaaa aggcaggtaa aaaattgtca
    19861 gatgtaaatt atatgctagg tggcgattta ataaatcaaa tagttccagc atcatttgca
    19921 gcaagagaac tggctattcc atttttaggt atatatggag cttgttctac aatggcagaa
    19981 ggtctttgta taggttctat gcttatagat ggtggttttg ctgatttagt actgtcaggt
    20041 acatcaagtc actattgtac agcagaaaga caatttagat tcccacttga acttggaaat
    20101 caaaaaccaa tgacagccca gtggacagtt acaggagcag gaagtgtatt attagctcct
    20161 aatggagacg gtccaaaagt aaagtatgtt actgttggta aagtaataga tgagggtatt
    20221 gatgatggta ataatatggg accagctatg gctccagcag caatagatac tatctactct
    20281 tactttgaag atactaatga tgacccaaat agctttgata taattgctac tggagattta
    20341 ggtactcttg gaaaacagat agcagaagac tttctaaaag aaaaaggtgt agatatatca
    20401 aaagtttaca ctgactgtgg tatagagatg tttaacctta aagaacaaga tgtccactgt
    20461 ggtggaagtg gatgtgggtg ttcagctaca gtgtttgcag gatatatata tgataagttg
    20521 agaaaaaaag aatttaacaa agtaatgctt gtatcaacag gagcactact ttcaccgaca
    20581 agtacactac aaaagcagac aataccatgt gttgcacatg cagtagtaat agtaaatgag
    20641 taggaagtga tataatatgt taatggacta tgtaagagta tttattgtag gtggaattat
    20701 atgtttaata gcccaagtaa tgatggatta tttcaaaatg caaactccat atgttatggt
    20761 aacgtatgta acaactggtg tagttttaac ttttctagga ctttatgagc cattagttaa
    20821 gtttgcagca gcaggtgcaa cagttcctat aataggattt ggatattcac ttgctacagg
    20881 agtaataaaa tcaattcaaa gtgatggttt tttaggtatt tttttaggag gtacaactgc
    20941 tacagctggt ggaatagcag cagctatatt ctttggatat attatgtcag tagtatttac
    21001 tccaaaagca aaaccataaa tctttattaa aagaataata agtttttgaa ataataactt
    21061 tttataaagc aggtggatat cttttagatt ccacctattt tttatataaa gaagcgttta
    21121 cataattgaa gttgacaatt atttacaaca tagtgtagta ttatattatc aaaataaaat
    21181 gaagtgaagt aggggataaa gatggtaact attaaagata ttgcaaggaa tctaggtatc
    21241 agttattcaa ctgtttctag gtgcttaaac aataatccta atgtatctga aaaaacaaaa
    21301 aagaaagtgg tagaagaggc aaatagacta gggttccatt ttaatgtaaa tgcaagaaat
    21361 ttagcaaaaa aagaaactaa caggataggg gtaatctttt caaataattt taatcatcaa
    21421 gatactagaa agttttttag tgatataatg gatagttcta taaattctat tgagactaat
    21481 aaatacgatt ttataataca acctaataat aacattagcg gtgatagtaa tgtatataaa
    21541 atggtaaatg gacaaatggt tgacggatta gtaattgtgt ctcaatcaat aaaaaaagaa
    21601 gagtatgatt ttttaaaaga caataatttt ccacatgtat ttatatattt taaaccatct
    21661 tttataggag aagtagacaa tttcttttgg gacgataacg tgtatggagg ttatatagct
    21721 accaagcatt taattgacca tggtcataaa gatataataa cagtaacttc agatgataag
    21781 agttcaaaaa tgcatgaaga tagaaccaag ggatatttga atgctatgga agaggcaaat
    21841 ttaaagaccg aagtcattaa atctaaaatg gattttgaat ctcaagttga atttttagaa
    21901 aaatacattg ataaaataaa aaaagcttca gctatatttg tacaacaaga tgtaccagcg
    21961 atttctataa tacaagagtt aaaaactact tatggtataa atgtaccaga agatatttcc
    22021 ataatagggt ataataatat tgaattaata tcatatttta ggtcgcattt atcaacaata
    22081 gatgacccaa gagagcaagt tataaaaaat ggagtagata gtttagtaaa tataataaat
    22141 aaaaagagta cagaacacat tccaagaaaa ttatatccaa ggttaataat acgaaatagc
    22201 gttaaaagaa taaagtagta agcttttaag tatcaaacgt ttattaaaaa tattatagat
    22261 tatgtgaagt tttttaacaa ataatataaa taaaaattaa aaggagtagg tgtatataag
    22321 tacattgtac tcctttattt attgagatag tttttataat atgaatgaat aattttattc
    22381 aattatttga aaatccgtta ttttaacaat ataaattaat aaattatttg tattttatat
    22441 catgaatcta taatattttt ttgattcata actaaaagta tataaaaatg atgaataaat
    22501 atatattata cattatcaat gataggttat ataataaaat cactaacaat tatattataa
    22561 gaaaattata aaaattacaa ggaggtatag aaatgaaaga aaaagcaggg attagaaatg
    22621 ccataatagc agcctttata atcacaatat tggcagaatt aattggacct atttcattta
    22681 aagttatggg aattaatgta actttactta gtatactatg ggctatattc ataggaatgg
    22741 ctgtttctcc tcatttatta gggagagtta taccagcctt aaaaaagttt ataggggata
    22801 atgaaataaa tgtatctcca tatttattat ctttgacact atatccatta gggataatgt
    22861 ttggaattaa tgcagggcct aaagttggta tattacttca agcaggacca gcacttttat
    22921 tacaagaatt tggtcatatg ggtacaatgt tgatagcatt acctgttgga gtagcattag
    22981 gtcttggaag aagtgcatta ggtggtactt tttcattatg tcgtgacaca gcactaggaa
    23041 ttattggaga taagtatgga ttaaactcac cagaaggtat aggaacacta ggaacttaca
    23101 taagtggtag tatatttgga acattattat tttcattttt agcaccagtg ggtgtacttt
    23161 taggatttca tccatatgct ttagcaatgg catcaggaat gggtagtggt tcaatgatgg
    23221 gagctgcaac agcatcatta atgaatacgg ttccacaaat gtcagagcag attttagcat
    23281 attcagctac tagtggattg attacagctg ttactggaat atatatagaa ttattcttat
    23341 ctttaccagt agccaactac tattatagca aagtagctcc agttattgaa agatttagag
    23401 gaagaaaaac aagaagaaca agtaataaat tgtaaaggag gatatatata tggaaaaaac
    23461 ttttaagact attatggagc agataaaaat agtattgttt atatatatta ttatttgctt
    23521 tggacaaatg atagcactta agactcaggc tcaagatttg ttgattggaa gtgtatctgg
    23581 atttttgttg gtgataattg ctattataat aaagaatttt gttaaaaaac ctaatttacc
    23641 aggttttgca tgggctacat tagttgcatt tgttttatcg cttccaatat ctccagtggg
    23701 ggatatcata gcaacaaatg tagggaaaat caattttatg tctacagtaa caccattatt
    23761 agcttttgca ggaatctctg taggtaacca attagatata ttaaagactt tgagttggaa
    23821 gttagtagtt atatcattta tagttatgac atctacttac tttggttctg cttttatatc
    23881 tcaaacagtt ttaagattaa atggaatgct ttaaggaggt agtaaatatg aatgaattaa
    23941 aagcaaaaat taaagaggaa ataaagttat tatctgaaga gaaaaagaca tcatttgaaa
    24001 aagtttctga ctatttattt tcaaatccag aattagcatt tgaggagtat aagtcacaaa
    24061 aagcattatg tgatttgtta gaagaaaatg aatttaatgt aactaaaggt gtaggaggat
    24121 tagaaacatc ttttgaagct gtgtattcaa atggaactaa tgggaaaaca gttgcttttt
    24181 tagctgaata tgatgcactt ccaggaatgg gacatgcatg tgggcacaat atcataggga
    24241 caagttctgt tggtgcaggt atcatattaa aagaaatcat gaaaaaacat aatatagaag
    24301 ggactgtaaa ggtttttgga acacctgcag aagaaagagt aggtggaaaa ataactatga
    24361 ttaaagaagg tgtatttaat aatgtagatg ctgcattaat tttacatcca agtgatgctt
    24421 caatgcctga tgatatatca tttgctcaag ttaatttaaa atttgatttt actggtaagg
    24481 catcacatgc tgctgctttt ccttgggaag ggaaaagtgc cttaagtgga gtaatagcac
    24541 tatttaacag tgtaaatagc atgagattac atcttaaaga ttatgcaaga gtacatggaa
    24601 ttataactga tggaggtagc atacataata taattcctga aaaatctaca gctatattta
    24661 atgtaagagc tttaagcata gaatatttaa atgagatttg tgaaatgctt aaaaattgtg
    24721 ctaaaggagc tgcaatttct actggaacta atgtagaaat agttcaatta gatgaaatat
    24781 acaaagagat taagaatgat tctgaattag tgaatatagt tagacaaaat tttgaagttt
    24841 tgggtgaaga ctatgttgaa agagatttaa gtcaaggtat aggttcaact gatacaggaa
    24901 atttaactca tgagatacct gcaatacaag catatataaa attaaaagaa aatactgcaa
    24961 ctcatacaga tgaatttgct gttgcagctg gtggtgaaga aggcaaagta gcacttataa
    25021 aagcaattaa ggtgctagca atgtgtggag tagatatttt gtatagtaaa taatatataa
    25081 ttttaaaata tattaagaaa ggaaatcaga ttagacatga tattaaaaca agtaatagag
    25141 gtttatgaca ttttagataa agcaaatgct aatggggaag aagtaaaaac ttacttacaa
    25201 ggttatggtg aagtagatgt aactgtaaaa gaattgtctt caagtaaagg ctcaacagat
    25261 ttagtaaagc ttacaattcc tgggaaaaat ggaaaattaa aaggtggaga tgctcctact
    25321 cttggcattt taggtagact aggagggatt ggagcaagac cagaagttat aggttttgtt
    25381 tcagatggtg atggagcatt ggtagcaatt gcagtggctg ctaaactttt ggatatgcaa
    25441 cgaaaagggg atatattaga tggtgatgtt gtaatttcaa ctcatatttg cccagatgct
    25501 ccaactaaag aacactatcc tactcctttt atggattctc caattgatat gatgactatg
    25561 aatgaaaatg aagttgatag tagttgtgat gcaatacttt ctatagatac tactaaagga
    25621 aatagagtaa tcaatactag aggatttgca atatcgccaa ctgttaaaga aggctacata
    25681 ttaaaaacta gtgataacct tttagatatt atgcagactg taacagggaa atctccattt
    25741 gtattccctt tgtctattca agatataaca ccatatggaa ataatctgta tcacttaaat
    25801 agtattttac aaccagcagt agctacagat gcaccaatag taggagtagc aattacaaca
    25861 gagatgcctg ttgctggatg tgctacaggt gcaactaatt ttagtgattt agattcagct
    25921 gggagatttg caattgaagt tgccaagcta tttggtagaa ataagtgtga tttctatgat
    25981 aaagaagagt gggaaatgct tattaagaga tatggcaaat taaatagatt tcaaacattt
    26041 ggtatacagt aattagaaaa atattaagtt tatataattt gtgatataac tatttaagaa
    26101 tatataaaca attaggaata aaagtaagaa taaagcactt agttaaaaac tttaaaaata
    26161 aaatatttat ttgtttatat aaaaaggagg attctggtta ttgggattct cttttttatg
    26221 aatacaaata atatattgta attataaaaa aaggagcgtt tcaaagtgat ttttttaatc
    26281 accttgagac aactccttat atttttattt aaattctatt ttttattaag ttgctggagt
    26341 tccaccacca tcaggaggag tagtagagcc accaccatta tttccaccat tatcaggagg
    26401 agtagtagag ccaccaccat tgtttccgcc attatcagga ggagtagtag agccaccacc
    26461 attatttccg ccattatcag gaggagtagt agagccacca ccattgtttc cgccattatc
    26521 aggaggtgta gaaccgccac cattatcacc tgtgttatta ttgttattat tattttcttc
    26581 tggttggtta ttgtctggtt tttttgtatc ttcagatgat ggttgagaac ttactttagt
    26641 aggagcagta ccttcaataa atgctgcttt agagccagaa gattgctttc ctccatcctc
    26701 taaattaatt tttgtaaaat atacattttt aggaacttca aattcagtaa ccgttaaatt
    26761 agcatgtatt ttttccataa ttttttccca tagttttgca gtactagtac ttccatgtgg
    26821 tacttctcta cgttttatag tgttaccaga atcatctttt ctaccagcat catctccaat
    26881 ataagttgca ccaacataat aaggagtgta accgacaaac caagcatcat atgcactatt
    26941 tgtagtacct gttttacctg ctacaggcat tccctttgga agtgtagcaa caccaccagt
    27001 accttcagta attacgcttt ctaacatatc tgtaacaaca taagctactt cagggtctac
    27061 aactttatgt tcctcaggag catttttaac taataattga ccatcaaagg tagttatagt
    27121 agtaaaaata gaaggttcta cataaatacc gtgatttgcc aatgtaccat aagcagcagc
    27181 catttgaagt ggagtaatac cattcgccat accacctagt gtcaatgatg ggaattttct
    27241 atcacttgct tcagatgaac ctgagtgatt attctttaaa gttgttatac caaagttctt
    27301 taagtaatcc atcattatat ctacacattc atcataagag tctccaagca tttcagcagt
    27361 tttgacagct atcgtgtttg aagatttagc aagtgcttta cgtaaactca tagaaccatg
    27421 accagcagta gtagttcttg gattccattt attattttct tcaaacttat atcctcctct
    27481 agaatcactt agagcagttg cttgtgttat ttttagagta tcaatagctg gcgtatatat
    27541 tgctaaaggt tttatagaag aacctggctg atgagggtta gtagcacgat ttaaagtttt
    27601 tctaccactt atatttcttc ctccagctag tgctcttatt tcaccatttt tataatctaa
    27661 gattaccatt gcagcttgcg gttggctaac tccatcagga ccaaccatat gtcctgggaa
    27721 attgctattt ctatcaaatt cttcttctaa agcatcttgc attttagggt ctatagttgt
    27781 gtttacaatt agaccaccat tgtagaataa gttttgagct tcatcatttg tatgtccttg
    27841 ttccattaaa gcatttataa cttcactttc gataaggtct tctacagatg aagaaacttt
    27901 atctgatgat tgaggcaatt ttatttctat tttagctttg attgcttcat catattcact
    27961 ttgttttata tatttcaatt ctaacatttt ctttaaaaca atttcttgtc tttttttagc
    28021 ttcaggatta cttacagcct ttctaacaac taatgtacct gcttttaatt gtctataagt
    28081 atcattagat ataagctccc aagattttat tttttcaatc atatcaaagt ccacttgagt
    28141 agggtcatct aaatcatcat cagttgtgtt aacaaaaaat aataatttat tctctaaatc
    28201 atccttagtt tcatttccct ctaatttaga agttttatag gctgaaaatc ttgatggatt
    28261 cttagttgaa ccagcaagaa gtgctgactc tgctagagta aggtcagctg cagacttatc
    28321 aaaataacca cgtgcaccag cctcagcacc tgctagacct ctaccaacga agaagttatt
    28381 taaataagtt tctagtattt tatctttact tactgtttta ctcatctcat gtgcatagta
    28441 aatatccttt attttacgag gtatagtttg ctgttcagtt gttaacagca ttttggatac
    28501 ctgcatagga atagtactac caccttgctt agtacctgta agtgtcttta atactgacct
    28561 aaataaacct ttaatatcga cacccttatg ctcataaaaa cgctcatctt ctattgcaat
    28621 tacagcatgt tgaagataag gggaaatctt atctaatgga acgggtgtct ttttatttac
    28681 acttggagct gtagataatg tttttccatt agcgtattta atagtagttg tttggttcgt
    28741 ctgtttatct aaaacagctt ctgtaactgg agttacatct cttagtgacg caaatactag
    28801 accagcagta cctgctgtac caacaactag taaaactaaa aatactatcc caaatactct
    28861 tagtttttta aatttatcgc tttttttaga ttttttcttg ttttttgttg aacgagtctt
    28921 gttagctgaa gttctattaa ttggtttatt tgtattacca gatgagctaa ctttttttct
    28981 cctgatttta tttccatttt tatcattatc gttattcata agattttaac ctccttactt
    29041 aatgcaatat tataacacaa ataggagtag ttttgtaatt aatagaaaaa atgaatttgc
    29101 tataatattc tgtacattgt atatgtataa acgtaatata ttaaaaagtc tatattttta
    29161 ttttatttat ttttgacaaa tattataaaa attatgccaa agctacatat aaataaatag
    29221 gaggggataa ttttgaatag aatagtagat agaaaaacaa aaaatgaatt tagaaaaatc
    29281 atagctattt ttttagtaat tcttttcttg tctgtattta taggcagttt tatatatata
    29341 gataaaactt taagaccgac aataacagtt ttagcagaga ctaaagctct tgaactagca
    29401 aataggtcga taaataaagc tgttgctgag atggtagaag gaaaaataaa ttatgaagat
    29461 ttaatggata tacaacttga taataatggt aaaataacta tgatacaagc aaacactata
    29521 atgatgaatg aaattgcatc tgcaatagct ttagaaatac aagatgaatt aaaaaaggat
    29581 aaaacagcaa gttcttacat acctatagga acagcattag gaagtcctat attagctaaa
    29641 tacggtccta aacttgaagt atctattgaa cctataggaa cagtatcagt aaactttaaa
    29701 acggaatttg agtcttcagg tataaatcaa actagacata gaatatattt agaagctcaa
    29761 acacaagtta aagtagtaat accacttata acttcaacaa aacaaattaa agctcaaata
    29821 cctatttgtg aaactataat agtaggtgat gttccagaaa gttatgtaaa tattccagaa
    29881 aaaaatctag gaaatgtttt acctaacact ggaaagaata ccaacaagta atataaagtt
    29941 gtatattttt aaataaattc aaaataatag tttataaata aaattttt
//
LOCUS       DAEMGG010000043        29475 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEMGG010000043 DAEMGG010000000
VERSION     DAEMGG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.043"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..392
                     /locus_tag="KPK24_001752"
     CDS             <1..392
                     /locus_tag="KPK24_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299426.1"
                     /translation="SKLILELLVITYFFIAVLATKYIFKGPSTASLPGLSWLIVFGCY
                     SIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNINISNLDTGI
                     ILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            393..605
                     /locus_tag="KPK24_001753"
     CDS             393..605
                     /locus_tag="KPK24_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2299427.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            1648..2481
                     /locus_tag="KPK24_001754"
     CDS             1648..2481
                     /locus_tag="KPK24_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2299428.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGREELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKNIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            2607..4448
                     /locus_tag="KPK24_001755"
     CDS             2607..4448
                     /locus_tag="KPK24_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2299429.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLSANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDIISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDRIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            4471..5904
                     /locus_tag="KPK24_001756"
     CDS             4471..5904
                     /locus_tag="KPK24_001756"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2299430.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGVIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPKGDESTPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     NRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAMPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     HTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNDSAGKA
                     DVVENPYLKISDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     DYRIEYLASHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            6609..7487
                     /locus_tag="KPK24_001757"
     CDS             6609..7487
                     /locus_tag="KPK24_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG2299431.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            7523..9418
                     /locus_tag="KPK24_001758"
     CDS             7523..9418
                     /locus_tag="KPK24_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2299432.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            9686..11593
                     /locus_tag="KPK24_001759"
     CDS             9686..11593
                     /locus_tag="KPK24_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2299433.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVSTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            11583..12032
                     /locus_tag="KPK24_001760"
     CDS             11583..12032
                     /locus_tag="KPK24_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2299434.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNANTKKEILDIFYEV"
     gene            12132..13019
                     /locus_tag="KPK24_001761"
     CDS             12132..13019
                     /locus_tag="KPK24_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2299435.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            13422..14870
                     /locus_tag="KPK24_001762"
     CDS             13422..14870
                     /locus_tag="KPK24_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG2299436.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMIIGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            14896..17286
                     /locus_tag="KPK24_001763"
     CDS             14896..17286
                     /locus_tag="KPK24_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG2299437.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVEDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSVGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            17288..17959
                     /gene="pgmB"
                     /locus_tag="KPK24_001764"
     CDS             17288..17959
                     /gene="pgmB"
                     /locus_tag="KPK24_001764"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG2299438.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            17998..19368
                     /locus_tag="KPK24_001765"
     CDS             17998..19368
                     /locus_tag="KPK24_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2299439.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQDVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            19715..20557
                     /locus_tag="KPK24_001766"
     CDS             19715..20557
                     /locus_tag="KPK24_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2299440.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            20702..22630
                     /locus_tag="KPK24_001767"
     CDS             20702..22630
                     /locus_tag="KPK24_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2299441.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            22691..24118
                     /gene="ascB"
                     /locus_tag="KPK24_001768"
     CDS             22691..24118
                     /gene="ascB"
                     /locus_tag="KPK24_001768"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2299442.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            24565..25341
                     /locus_tag="KPK24_001769"
     CDS             24565..25341
                     /locus_tag="KPK24_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2299443.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            25357..25722
                     /locus_tag="KPK24_001770"
     CDS             25357..25722
                     /locus_tag="KPK24_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2299444.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(25888..26637)
                     /locus_tag="KPK24_001771"
     CDS             complement(25888..26637)
                     /locus_tag="KPK24_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG2299445.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            27095..27520
                     /locus_tag="KPK24_001772"
     CDS             27095..27520
                     /locus_tag="KPK24_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2299446.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            27552..28739
                     /locus_tag="KPK24_001773"
     CDS             27552..28739
                     /locus_tag="KPK24_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG2299447.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            28931..29335
                     /locus_tag="KPK24_001774"
     CDS             28931..29335
                     /locus_tag="KPK24_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG2299448.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPLIYLKYKPDK"
BASE COUNT        11720 a         3024 c         5207 g         9524 t
ORIGIN      
        1 gatcaaagct catcttggaa ttacttgtaa taacttattt ttttatagct gtattagcta
       61 ctaaatacat ttttaaaggt ccaagcactg cttctcttcc agggttgagt tggttaatag
      121 tttttggatg ttattcaatt tataataaaa aatataatga tttagttgat gaatcatctg
      181 aatttatact atcaaaggtt aataagattg caataaaaat aatgatatat tctatagtaa
      241 taatatctat tttctttgca acaccttata caagtaatat aaatatatca aacctagata
      301 caggtataat cctcattgca gttttattta tacagtcttt atctaaatta gtgttgtata
      361 tatactttga taaaaggggg atatgtaact aagtggcaat attgatgaaa acaaaaatta
      421 aagaatacag agaaaaactc cttatgacgc aaaatgaact tgctaaattg gttggggtaa
      481 gacgtgagac tattgtacac ttagaaaatg ggaaatataa tccttctttg aagttagcta
      541 tggatatcgc taaagtattc gatactacag tagaaaattt gtttgaattt attgaagaag
      601 aatagtggaa agtttataaa catttataag atggataatg aaatatagtt atttaattat
      661 taagtttatt aaaaatattt atataatatg tgattaaggt tagattataa ttgttttaag
      721 ataataagaa gagtattata aatgataaaa aatagtgaat atacagttaa ttaataaaca
      781 atagataaaa atttaaagtt taaatgtaaa gaggatgtat aattttatga aatctaataa
      841 gacactgtta taatcgctat gtaaggaaat catattagaa ttttctaaga aaacagttaa
      901 gaatttggaa gtaagtatgt gttatttata tacttctgaa acatataaac taataagtga
      961 ggttgtatca gatatgtctt gtaagggtaa tttcttcagg ttcatcaact atcataactc
     1021 tacagagggt ataagatatg ctttgtaagg gtaatatatg cttgattcaa gaattagtgt
     1081 tataaaatgg attagcatac caaaaggtct attaaagata tacagttaaa aagctatata
     1141 gtaagttagg taaggttaat aatccaaaca atgatttgat gattaaactg tttagagcct
     1201 tagatacaac agtaaaagaa ttatttttta ataaggagaa gtaaaagata aaagaaaatc
     1261 aaaacttagg cagttattgt agttacaaaa taaaggattt ccttatatat ggataaataa
     1321 tcgagtatat ataataaaaa atatatgacc ttaattttgt tatgaaataa cttcaaaact
     1381 tattttagca tgtttagatt atataaatca tataaaaaag tagaatgagt gaatacttat
     1441 gacaataatc tagtagagat atacatgaaa ttatatattg actaagtaca gttattattt
     1501 tataatgaaa ataaagttat catacagata aataaacgga tcgttactga gatagatgta
     1561 atcaggcgtg acctaaaaat tctagtaaat gcttaaagta ttattagtct tttttaggtc
     1621 tctttttgtt tacagtggag gtagtttatg gttataaaga aagtatttaa taataatgtt
     1681 gtatttgcca gagataattc aggacaagaa gtaatattaa tgggaaaagg aattggattt
     1741 aataaattta ctaaagattt agttgaaata gataaaattg aaaagaaatt tatattcaat
     1801 aatgaggaat cattaagtga attacattca ctactagata ggataccttt aaaagatgtt
     1861 gagcttgtaa gtgaaattat tcaaatggga agagaagaat tgggtgataa tcttaatgat
     1921 agtatattaa tcacattatc agaacatata agttatatgt taaaaagaat ggatgaagga
     1981 ttagtatttc aaagcccgct tcaatgggaa ataagacaaa tatatcctaa tgaatataat
     2041 tttagtaaga aagctgtaga atatttaaga aaaaaattag ataaaaatat tcctgattct
     2101 gaaatagcat ttattacgat acactttgca aatgctcagt tagaaacaaa agatatgcaa
     2161 gaaactttat tgttaactaa gataattgat aatgtattag atatcataaa gtatcactat
     2221 ggaatcgaaa taaatgaata ttcctttgac tacacaaggt ttataagtca tttaagatgt
     2281 tttataaaaa ggcaattaag tggtgagaat acaaatgtag acacatcatt gttagatata
     2341 ataaaaatga aatataaaag tgattacaat tgtgcaaaaa aagtaaaaaa ctttcttgaa
     2401 aagacgtata aatggaaaat tagcgaaaat gaattgttgt acttaacatt gcatttaaat
     2461 agattatcaa atataaagta aatagtgtag gattgttact gtttggcagg catgacctaa
     2521 gttggtataa ttagttttta atatctaatt gtattgattt aggtctattt ttatacataa
     2581 aattatgaaa atggaggata gattgtatga aatttgaaag agagtctgaa gaaattataa
     2641 aatatgtcgg aggaaaagaa aatattatta gtttagttca ttgtgcaact aggctacgtt
     2701 ttaagctgaa agatacttca aaaactaaca aagaagagtt attaaaaatg aaggatgtac
     2761 tatctgtagt aaatagtgga ggacaatacc aagtggtaat tggaaataaa gtaactgatt
     2821 actttgaaac tattataaaa aaagcagttt tacaaggaca aaatactgaa gaacagggag
     2881 aaaaagtttc aatagtttct aaaatatttg aatcaatttc aggtgcattt tctccattaa
     2941 tacctgcact agctggagca ggaatgatta aggcactgct tacaatacta actactatga
     3001 aatggatgtc tgatacaagt agcacttatg ctatattatc agcagctgga aatgctgtat
     3061 tttacttttt accaatattc ttaggtatta ccttagctat aaaacttagt gcaaatccat
     3121 atgttggtgg agtgatagga gcagctttgt tagatcctaa ttttacagct ttaattggag
     3181 caaaaggtgg tgctaacttt tttgggatgc cagtaacagc aattgattat tcttttggtg
     3241 tattccctat atttgttgct atatttattt attactatgt tgataagctt ttaaagaaga
     3301 ttatattaaa ggatttacaa ttattcttag taccgatgat atcattatta gtcatggttc
     3361 ctttaactgt gattttattt ggaccaatag caactaatgt tggtgatata atatctaata
     3421 tttttatggg attatttgaa tttaacagag taattgcagg agctgcaata ggtatagcta
     3481 tgccattctt agcagtatta ggactacact ggggcttcac accaattact ttgcaaaact
     3541 tggctacaat gggtggagac ccaatagaag gtgcagctgt tgcagcagta tttgctcaaa
     3601 taggtattgc aataggttta tttatgaagg ttagaaaatc taaaaaaaat tcagaattaa
     3661 aggtattagc aggttcaaca gcagttacag gaattttagc aggagtaaca gaacctataa
     3721 tttatggaat tatattaaga tacagaagaa tgattccagt attggctatt gcaggaggta
     3781 ttggtggtgg tatatgtggt gtatttggtg ttacttgtaa tgcatatgta tttcataata
     3841 tattctcaat gccagtttat acaccattcc taggatatat attaggaata ggaacagcat
     3901 tagtgctagg agcagtattg tcatatttct ttggattgag taaagaagaa tgtgaagaag
     3961 gtaatgaaga agttgctttt gatgaagatg atggtataga ggagatatta tctccaatag
     4021 aaggcttaca aatagagtta tctgaagtag aagatgaagt gtttgcatca ggaactatag
     4081 gtaatggcat ggcaataata ccttcaaagg gagaagttgt gtctcctgta aatggaacaa
     4141 taacaactat ttttcctaca aagcatgcaa taggaattac ctctgataaa ggtgtagaaa
     4201 tattaataca cgtaggtctt aacactacta tgttagatgg aaagtattat actactcata
     4261 tgaatgcagg agataaagtt aaaaaaggag ataggattgt ttctttcgat aaggataaaa
     4321 tagaaggtga agggtatagt attgttacac caataatact aactaacaca aatgatttag
     4381 cagatgtaat agttaaagaa aataaacata ttaaaattgg aacgcctgta ataaaagttg
     4441 tttattaatt atactggagg agatatttct atgaaatcta atcaagaatt tttatgggga
     4501 ggggcagttg ctgcccatca agttgaaggt ggatataata aaggtggaaa aggaataagt
     4561 attgcagatg tgatgactgc aggtactcat actatttcaa gaaagataac agatggagta
     4621 attgagggtt taaattatcc taatcatgaa gccattaatt tttatgaaaa ttataaagaa
     4681 gatattagat tatttgctga aatgggatat aagtgtttta ggacaagtat tgcttggact
     4741 agaatttttc caaagggcga tgaatcaact ccaaatgaag atggattgaa attttatgat
     4801 gatttatttg atgaattatt aaaatacaat atagagccag taataacttt aagtcatttt
     4861 gaaatgcctt atcatttagt aaaaaactat ggtggatgga gaaatagaaa attaatagat
     4921 ttctttgtaa acttctgtga agtggtaatg aatagataca aggataaagt taagtactgg
     4981 atgactttca atgaaataaa taatcaaagt ataactacaa atcctattta tgcatttact
     5041 aactcgggaa taatctatga agagcaggaa gacaaagaag aagttatgta tcaagcagtt
     5101 cattatgaat ttgtagcaag tgcaaaggtt gtgaagatag gtcatgaaat aaaccctgag
     5161 tttaaaattg gatgtatggt ggcagctatg ccaagttatc cttattcttg caatccagag
     5221 gatatgatta aatttgtaga atctaataga gaacaactta tgtttactga tgtacatgta
     5281 agaggtcatt atccaagaca tacactaaaa ttatgggaaa gaaaaaatta taatttagat
     5341 attacagagg aagataaaaa aatcctaaaa gaaggaatag ttgattttat tggatgtagt
     5401 tattatttaa ctactgtagt aacagctgat aaaactatga aaacaacagg taatgactcg
     5461 gcaggaaagg ctgatgtagt tgaaaatcca tacttaaaaa tttctgattg gggatggaat
     5521 atagaccctg taggtctaag gttttacctt aatcaactat atgataagta tgaacttcct
     5581 atattcattg ttgaaaatgg atttggagca gaagatgtat taaaatcaga taatactgta
     5641 gatgatgatt atagaataga gtatttggct agtcatataa gggaaatgaa aaatgccatt
     5701 gaaattgatg gtgttgatgt tatagggtat actgtttggg gatgtataga tccagtatca
     5761 tttacaacag gagagatgaa aaaaagatat ggatttatat atgtagataa aaataatgat
     5821 ggaagtggga ctcttaaaag atataaaaag aaatcttttg attggtataa aaatgtaata
     5881 aaatttaatg gagaaatttt ataggtagat aaattttaat atttataaga ttatgttaag
     5941 gaaaccatta aaaattagat taaaatcgtg ggtatttaaa tccactaaaa gatattgact
     6001 atttagtcaa tatctttttt tattaaaaag taaatataaa gaggtaaggt taaaaattgg
     6061 gatatatatg atttatatag tatgatttaa ttagaaggag ctatattagt attcatttgt
     6121 atagttggag aataaatgtc agtcttacct tacgattttt ataatagtcc tatttaattt
     6181 ctattgtaac taatatgcaa gaatttaaga tagaagtatc tatgacttat ggtgtattaa
     6241 taattggtat ctgctatcta gtatcttttt aatttttttg attccaatat agtgctaact
     6301 acgatacgaa tatctttaaa tttatagcta aactatctaa ttgtttattg gaaagagtta
     6361 agaaggctaa agcataaaac acttaaaaag agaagatgta tatgagaaat ataattaact
     6421 atgatgcaga cttctaaaaa ttatttagtg aaaaggaact tagagtgtat aataaaagtt
     6481 ccttttcact aaggaaaacg attgttattt tctgatcttc attaccctga tattatttaa
     6541 atataaacaa caacaaaatt aacaagaagc aaattcaacc taaaatttaa aatatcggga
     6601 ggtacaatat gttaataaat atgaaggaaa tgctaaaagt ggcacaagaa aatcaatttg
     6661 ctgtaccagc atttaatata ggaagtgggc aaatacttaa agcagtagta cagagtgcta
     6721 acgaaaaaaa tgcaccagta atacttgcaa tacacccaaa tgagctaagt tttctaggag
     6781 atagctttgt agctagttgc atagaagaag caaataagag taaagtacct atggtaatac
     6841 acttagatca tggggaaaat aaagaacaaa tattaagggc aataagatgt ggatttacat
     6901 cagtaatgat tgatggctca catttaccat atgaagagaa tgtagctata tctagggaaa
     6961 tagtagaaat agcaaaagga ttaaatgtgt ccgtagaagg agaacttgga acaataggaa
     7021 caacaggaac atcaagtgaa ggtggtactg atgaaataat atatacagat tcaaaattag
     7081 ctaaggattt tgttgaaaaa acaggagtag aaacactagc tattgcaatt ggtacagcac
     7141 atggtatata tccaaaaggt tttaaaccag agcttaaact agatttatta aaagaaataa
     7201 gagaagtagt agatatacct ttagttcttc atggtggttc atcaaatcca gatgaagaaa
     7261 tagcacaagc agttaaacta ggagtttgta aaataaatat ttcgagtgat gttaagtcgg
     7321 catattataa gaagtgcaga gaactattag aacaaaaccc aagcttatat gagccagata
     7381 ctatatatcc accatgtata aaatctgcaa gagaagttat agagtttaaa atgaatctat
     7441 tcaatgctat agataagtta aagtgctttt ataaaaataa aaattaatta tgaggtaaca
     7501 aattaaaagg aggggagaat ttatgatagg taaaatacta cgaagcgatt gtattgtact
     7561 agataaacaa ttacaaacaa aaaaagatgt aattaattat atggcaaatc atttgtacca
     7621 tggaaattat ataaataata aagaaaagtt tatagctgat attaataaac gcgaaaaaga
     7681 atcatcaact aatatgggta atttaatagc tatacctcat gctaagtcaa atgtagtaaa
     7741 tcaacctgta atgatgtatt taagaacaga tagagagatt acagataatg atggtcaaaa
     7801 acttaggtca ttttttatga tagcagttcc agaatcaggt tcagatgaac atcttaagat
     7861 tatatctaaa ctagctacat atctaatgga agaaaatttt cttaagcatc tactaagtgc
     7921 agaaactcca tacgaaatat taagtatatt taataataaa gctgcagaat caggagattt
     7981 tagtaataat caaaataaag gtcttatagt tgggataaca ggatgtccaa caggtatagc
     8041 tcatactttt atggcaaaaa aagcattaga ggaagcaggt caagaacttg gatatgaagt
     8101 taaaatagaa actcatggag ctataggtgt agaaaataag ttgactaaag aagatataaa
     8161 aagagctgaa tacgtaataa tagcttgtga taaagaagtt caactgagta gatttatagg
     8221 aaagaaagtg ctacaaaaga aagtagtaga tgctaagaat gtttcattag ctaaaaattt
     8281 aattataaac gctgaaaatg gaatggctaa agaaattaaa ccaggtggaa agactttaga
     8341 agattcaaaa gaaatgcttc aagggtcaga gaaaaagggt gtttataagc acttaatgag
     8401 tggtgtatct gaaatgattc catttgtagt gataggtgga attggtatag ccatagcttt
     8461 tatgtttggt atatttgcat cagacccaac tcatgaaaca tacaacccta tagctggatt
     8521 ctttggacaa cttggaggcg attcagcatt taagttattt ataccgatac ttgctggttt
     8581 tatagctaaa tcaatagcag gaagacaagg atttgctcct gctatgatag ctggttttat
     8641 ggcagttatt ggaggttcag gttttatagg tggtatggta gctggttttg caacaggata
     8701 tatagcttta ggagtaatga agataacaga aaatttacca gatgtattaa aaggagtaaa
     8761 tgctgtattg atatgtccac ttttgattgc atttacgagt ggagctataa tgttctttgt
     8821 agttaatacg ccagcatcat ggttaaatga agctttacaa tcttggttac aaagtatgtc
     8881 tggaactaat aaagtattat taggtgcaat attagcaggg atgatggcat cagatatggg
     8941 aggacctatt aataaaacag cttcagcttt tggattggct atgttttcaa gtaatatatt
     9001 tgaaccatca gcagcattaa tggtaggagg aatggtacct ccattagcaa tatcattagc
     9061 tatgttaata ttcaaaaata agtttactaa agaagaaaga aagtcatctc taccaactgc
     9121 tataatgggg gcatctttca taacagaagg cgcaataccg tttgcagctg cagacccatt
     9181 aagaatgatt ccagcaaata taattggttc ttgtataggt ggggcaatat gtatgagttt
     9241 agatataact ttaatggcac cacatggggg aatatttgta ataccatttg catgtagtgc
     9301 accagttgca tatatagctt gtatattgat tggtacagta ataacagcat tgattatagg
     9361 ggtaacaaag cctactataa ggagtgaaaa tagtgaagtt gaattacttg gtgcttaaat
     9421 tataaataat agtaaaaata attaatggaa taacaacaag tatatagaat aataataagg
     9481 atatttagaa tcagttaact acttctaaat atccttaatt tttattttga aattatatta
     9541 tctattatat caatttagta aggtgtatca taaattcttt tatgatacag aagtatctta
     9601 gtaattttac atagttagta aatatgttaa aatagtactt aatataaaca tagaaatata
     9661 taaacatagg tagggagggc taagtatgct tgtgtcgagg caaattgaat ttcttaaatt
     9721 gatactaaac gaaaataaat atagacctat aaaatacttt aaagataaat taaatgtatc
     9781 tgataaaact ttacaaaaag atttgaagat gattgagaaa tatttagaaa cttttaatat
     9841 aaaaattgat ataaaaagag gctatggaat attaatagaa gatttagcaa agaagaatat
     9901 agacttaatt aatagtttaa atatacaatc gaaggaaaat aaatctttat cagtagagca
     9961 gagaagaatg gagattttaa agtatttatt actaaattca aataatataa cttcaataaa
    10021 tcagttagca gataaatatt atgtaagtaa aacttctata gtaaatgatt ttaaatatat
    10081 agaggcatgg ataaaagaat acaatttaaa acttaataaa actttagaag gaactaagat
    10141 tataggtaaa gaagtggata taaggaaatc gattgccaaa atgatggatg atttattaga
    10201 tgaaaattat gaacaacatg atatatctga gttaacaaga ttagattctg ctacatttag
    10261 tgcattgata aatttatttg atattgattc tattatattt gtagaaacta taattacaga
    10321 attagaatac aatttaggat atactataaa tcagccctac tatataaatt taattaccca
    10381 tatacttata tgtttaaaga gaatcgaaga aggtaatcaa atagaatctc aggaagaaag
    10441 agaaatttct gtagataatt tggacgagtt agtatataaa aatgtcagta cacttataga
    10501 taaaatagaa aatagataca gagtaaaaat aacgaaagat gaaattatgt acatatatat
    10561 ttttttggta tcatctggat ttagtagcga aaataatagt agttacaatg atgaatcaga
    10621 tagattaagt gaaagtgaaa aagtttcatc tataatgata gagaatatgt cacgattttt
    10681 aaatataaat ctcaaaaatg atgacttact acagaaaagt ctagcatctc atgtaaggcc
    10741 tatgttaaac agattaagat atgatatcca aataaaaaac ccgttgttag gagagataca
    10801 agagcgattt tcagatgtgc tgggattatg tatgatgact ataaatatta tgacagagta
    10861 tgataatctg aagaatatta gtatagatga agtttcgtat cttgccacat attttcaagc
    10921 ggcaatagaa agaaatatga gtagtaaaag agtaatagta gtttgtcata caggatatgg
    10981 aacatctcag ctccttgcag ccagattaaa aagagaattt cctgaatgga ctatagttga
    11041 tatagttcct atgcatcagt tgagtaagcg aaatttagat gatgtagact ttgtaatatc
    11101 tactgtaaac ttagatttga aagataaatt acatatagtt gtatcagtac tattaatgga
    11161 aaatgatatt aataatataa aaaatgcttt actttctaaa cctaaaaaaa gtatgaactt
    11221 agatacaaac ttattaggaa tgtatttaga agataatata tattttaatt ttgatgtagc
    11281 tcagatggaa agtttaatag gtgtgaattt agggagtaaa tatgaatcta tttcattagg
    11341 aaaagattta aatatatata tacataagca aagtaaatta aataaaggga taatgaatat
    11401 caataatgtt actagaacga ttgatattta tttagaaatt agtaatcaaa gttttttaaa
    11461 gaatatttta atggaaatat caaatttata taggcaaaag aactatataa actatataat
    11521 agattgcaag agcaaagagg atattaaaat attatttaag aataatctat tggagaaaac
    11581 aaatggaaat tagagaaata ttaacagaag aaagcattat tttagatttg aaatcaacta
    11641 caaaagaagg cataatacag gaattagtag ataaattata cgatacagga agtataactt
    11701 taaaaagtaa gtttataaag gcattaaaac atagagaaaa aataggctct actagcatag
    11761 gttatcaaat ggccatgcca catggaaaaa caaaatatgt aaagaaggca tccatagtaa
    11821 taggtaaatc taaaaaaggt gtagattggc caagtataga tggcttacct gtaaaggtaa
    11881 taattatgtt tgctatgcca caatctggag gcaatgaaga tatgatatta atatctaaaa
    11941 tagcttacat tttaggtggt aaaaatatag gtgaaagact tttaaatgca aatacaaaaa
    12001 aagaaatatt agatatattt tatgaagtgt aggtattgta taaattataa atttgaggtt
    12061 tttaaaataa aatgtgtatt cttttaaata gtataaatta atttttagac actagagagg
    12121 agaggatata tatggcaata tatttagaaa tgcctaaatt aatacatgaa tttccttttc
    12181 gtagtctatc aaatgaagga gaagtattaa caacaccaca ctggcataag gaaatagaaa
    12241 tattacttat aacagaaggt gtagtaaatc tatttataaa tgacaagcct atgcaattaa
    12301 aaaaaggcga gatagtgata ataaaaggag gagatattca ttatatactg ccatcaccgg
    12361 gaagtgaaag attagtattt caatttgata tatctttttt taatgattta attttattaa
    12421 atgaggaaaa agaaagctta attaatttat tcagtagtat aaaaaaatgt agtagagatt
    12481 ggtcagcaaa aataagaaag tcagttttcg atatattgat agatatctac aatgaagacc
    12541 tatacaaaat agaaggatat agttatgcaa taaaaggtaa aatgtataat ttaattccta
    12601 tattatatag acagattcct agagaaattg aagaaaatac agtagaatat gaaataaact
    12661 ctaaagagat attagagaga ttgaacaata tattcaaata tgtagaaaaa aattataaac
    12721 aacctataaa attagaagat gtgtcttatg aagtaggatt tagtgtatat tattttacta
    12781 aatttttcaa aaaaaataca ggaaaaacat ttataacttt tttaaatgaa tatagattag
    12841 aaaaagcaaa gtggatatta cttaataaca atatatccat tgtagatttg gttgaagaag
    12901 ttggttttgg aagtacaaaa actttttaca gagtatttaa agaaaaaatt ggagtctctc
    12961 cattagaatt taaaaaaaga tataatagct tggaaaaaaa agggagtgtc tcaaaatgaa
    13021 cttagttcat gaggcacttt cttttatatg aaatcaaata tattttcagt atttcaacaa
    13081 tgaaaattcc attcatttat aaagtctaaa ttttagttct caatacaacc tctatttcga
    13141 aataaatcct tttatatata aatatacagc aatatatgag aataagataa caataatgag
    13201 gatataaaaa tgtatttgct atatatataa ttatatacat agatatctaa tattgatatt
    13261 taatatgaaa gtattgaaaa tagacaatat taattatagt gagtaaaaaa tataattgga
    13321 tttttaaatt gaattattta aaaggtatag tatgaaaaaa atcttataac taaatatgag
    13381 atagaaatta taaaacttta tattgtgaaa gaggtagatg catgaaatta gggaaagatt
    13441 ttttatgggg aggggcaatt tcagccaatc aaagtgaagg attttattta gatggaggaa
    13501 aaggtttatc tcagtttgat aaactcccat taaatgataa gagattaaaa gatgtatgtt
    13561 tagatgagga taatatttta aatgaaaata atcaatacta tccaagtcat atagggatta
    13621 aattttgtga aaattacaaa gaagatatag caatgttagc agaaataggt tttaaatgtt
    13681 ttagattttc tatatcttgg tcaagaatat tcccaacagg agaagaaagt gaaccaaatg
    13741 aaaagggatt acaactttat gataatataa taaaagaatt aaaaaaatat aatatagagc
    13801 cattaataac gataagccac tttgatatac cttattctct agttgaaaaa tattctgggt
    13861 gggctgatag aagagtggtt aatttatatg taaaatatgc tgaaattttg atgaaaagat
    13921 ataaagatga tgtcaaatac tggattcctt ttaacgaaat gaatatgata atgcatattc
    13981 catatatagg tgctgggtta acttttatag ctgatgaaaa tcaacttcag aaaaaatatc
    14041 aagcagcaca ccatcaatta ttagctaatg caaaaactat agaaatagga aggtatataa
    14101 ataaagaatt taagtttggg tgtatgttag ctgctggaaa aacatatcca tacacatgta
    14161 aaccagaaga tgtatttgca actttagagc atgaaaggca taatttgttt ttttctgatg
    14221 ttcaagttag gggtgtgtat ccaagcttta tgaattatta tttaaaatca aatcaaataa
    14281 aattaataat aaaagaggaa gatttatcaa cacttaagga taatactgta gattttgtat
    14341 cttttagcta ttattctagt gcttgtagtt cagctagagc tgaaggttta gaaaaatcaa
    14401 aggcaaacgg accagaaacc ataaaaaatc catatcttcc aaaatcaaat agtgtatggc
    14461 aaaacgaccc tgtagggtta agaataacta tgaatcagtt atatgaaagg tatcagttac
    14521 ctttatttat agtagaaaat ggactgggta ctcaagatat tgtaaatgaa gatggaaaaa
    14581 taattgatga ttatcgtata gattacatga aagaacatat taaaaatatg attattggag
    14641 taaatgaaga tggaatagag cttataggat atcttatgtg gggatgtata gatgtaacca
    14701 gtgtaagtga aggaaagatg acaaaacgat atggcataat tcatgttgat tctgatgact
    14761 atggaagagg ctcatttaaa agaactaaaa aacaaagtgc tttttggtat aaaaaagtaa
    14821 tagaaacaga tggagaaatt ttatttaatg acatggaaac tcatgcataa ttcattttaa
    14881 gaaaggagtg ttattatgag tttaatattt actactaagg accaacttat agagcaaggg
    14941 tttagtaagg atacaataaa atccaatgaa acgctgttta cactagctaa tggacattta
    15001 ggagttagag ggaatattga agagtctgat tttaataatg agtatgtaga taatatgggt
    15061 acatatgtaa atggatttta tgaagaatct ccaattacat atggagaaag cgcttatggc
    15121 tatgcaaaac aaaatcagac tatctgtaag ttacctaatg ctcatatagt aaatttctct
    15181 attgaaggag attgctttga tttatcaaaa ggtattacaa gtgaacattc taggatacta
    15241 gatttaaatg aagggattct taaacgttca tttatatggg aaaactctcg tggtagaaaa
    15301 gttaaagtat ctatagaaag attagtatca caagatatag aagaattaat gatgatttca
    15361 tatacaataa caccattgaa ttttaatgga aaaataaaat ttaaaaataa aatggaaaat
    15421 agtatggaga agtttaattg tgaaaaagta gaggacccaa gagtggcatc tataaataag
    15481 aagcaattta aaattaatgt aaaaaaatat aacaataagt attttatgaa tttagttaca
    15541 gaaagaagta gattagggtt atggtgtggg tttgattcat atattacttg tggaaatatt
    15601 acaaatgaga cctataatgt ttcagatgaa tgtattgagc gagaaataga agtctatgcc
    15661 aaaaaagatg aaaggttaaa tttagaaatt gttgtaggct atagtagagt atattctgat
    15721 ttaaatctaa atgaagaacg tttaaaatca ttagaatgtc tggagtcgtt aaaatgttta
    15781 gaatcagaag cacatttaaa atcattagaa ttggttttaa gagaagcatg taatattgga
    15841 tttgataaag tcaaaaatat tcagagaaag aaaatgaatg agttttggac aacctctgat
    15901 gtagagattg aaggcgataa aactcttcaa ctggggataa aaaccaattt atttcatatt
    15961 tatcaatcag taggaagaga tggaaagact aatatatcag caaaaggtct tacaggtgat
    16021 ggctatgagg ggcattattt ctgggataca gaaatgtata tattaccatt ttttatatat
    16081 actcaaccat ccattgccag agctttatta gattatagat actcaatctt agatgcatca
    16141 agagaaagag ctagagaact tggaataaat aaaggttgct tatattcatg gagaactata
    16201 aatggtaatg aatgcagtgc atattatcca gcaggaacag cacagtttca tataaatgca
    16261 gatatagctt atggtattag aacgtatttt gaagcaacta atgatgaaga atttatgatg
    16321 caaaatggct tagaaattct tatagaaaca gcaagatttt gggttgaatt tggagattat
    16381 atagaatcaa aaaataataa attctgtata aatggagtaa caggaccaga tgaatataca
    16441 gcaatagtaa acaataatta ctacactaat ttaatggcta agtataattt aatatatgca
    16501 gtacagatgg tagataaatt taagcataat ttattttcaa atgaagtatt tagaaaagtt
    16561 aaattacaag aatatgaagt gaaaaactgg ataaatgccg ctcaaaatat gtatttacca
    16621 tatgatgaag agaagaaaat aagcaaacaa gacgattcat ttttagaaaa atcagtatgg
    16681 gattttaaaa atacaccaaa agaaaaatat ccattacttc ttcattatca tccattaaca
    16741 atatatagat atcaagtaaa taaacaagca gatactgtac tttcagcgtt gcttttccct
    16801 gatgagttta gcttagaaca aaagaagaga gattttgaat actatgaaaa aataactact
    16861 catgattcat ctttgtcaag gtcaatattt ggaatgatgg caagtgaaat tggaaatcat
    16921 gaaaaagctt ataactattt tatggacaca gcattaatgg atattaataa cctacaaaaa
    16981 aatacaagtg atggaataca tgctgctaat atgggtggaa catggatgag tttagtatat
    17041 ggatttgcag gaatgaaaat aaaaaatgat aaattaagtt ttgaaccaag gatacctaaa
    17101 cactggaaaa atattaaatt taatatatta tttagagata atttagtttc tgtaaatata
    17161 gaagaatata aatcttatta taagttaatc aaaggtagaa atataaccat attacatgat
    17221 aataaagaaa taaaattatc aaagaacata atagaagtta ataattgttt taaagggagt
    17281 aattaatatg aaagcagtct tatttgatct tgatggagtt ataacagata ctgctaagta
    17341 tcattttgaa gcttggcatg agatagcaaa atcgctagat atagaaatag atgaaacttt
    17401 taatgaaaaa ttaaaaggca taagcaggga agaatcatta gataaaatat tgttatctgc
    17461 taataaagta gatagttata ctaaagagga aaagttaaag gtgtgtgaag aaaaaaataa
    17521 catttatata aagttaataa actcattaac atataaagat atattaccag ggataaatga
    17581 gcttataaaa gaacttaaaa aaaataatgt gaaattggga atagcatctg ctagtaagaa
    17641 tgcacctagg atattagaat ctttgggaat aaaagatgaa tttgattgta tagtaaatcc
    17701 atctttatta aaaaggggaa aaccatatcc tgatattttt attgaaggga gcaagattct
    17761 aaatttaaaa caatatgatt gtgtaggaat agaagattct tattcaggta ttaaagctat
    17821 aaatgctgct aatatgacat caattggtgt ggggaaaaaa gaagtactag gaaaatgtga
    17881 ccatattgta gaagatacta gcttattaaa ttacaataca ctattacaag cttggagtaa
    17941 aaagattaat ttggattaaa attatattaa aaattatttt aaaagagagg ataaggtatg
    18001 aagaataaat atgaagaagt agctcaattg ataattgatg gacttggagg aaaggataat
    18061 ataattgact taactcattg tatgacacga ttaaggttca tcttaaaaga tgaaaagaaa
    18121 gtcaatgcag ataaattaaa atctatagat aaggtagttg gagtaaactc aacttcaact
    18181 caatatcagg tcataatagg taatgaggta ggtgttgtat ttaatgcaat aataaataag
    18241 gggataaata ccacaggaga tattaaaagc gatataaaaa ataaaaataa aaaaaatagt
    18301 attttaaata atataataga tacaataact ggttgtatga cacctatgat accagccctt
    18361 acagcatctg ggatgataaa agttatatta tctctttgca ccacttttaa ttggctatca
    18421 tcaaattcaa gtacatatag agtattagat ataataggag atgtagcatt ttactttatg
    18481 cctataatac ttgcaataaa tgcagctaaa aaatttaatg ttaatacatc aatagcagtt
    18541 atagtagtag gtgtgttttt acatccaaat ttctctgcat gggtatcaag tggggaccct
    18601 atatcattta taggagtacc aattcaagat gttatatatg cagcatctgt tatccctgca
    18661 cttttaacag tatggatgat gtcatatata gaaaaattca ttgataaatt aacacctagc
    18721 atgttaaaaa caattttaaa tccaacttta gtattattaa taagtgcgcc aatagcatta
    18781 atagtaattg gaccaatagg aaatttctta ggagagggat tagctagtat aataaattta
    18841 ctacaaggaa ggttaggatt tataatggtt tgtttattag cagctgcaat gccatttatc
    18901 gttatgacag ggatgcatca tgcattgact cctatattca tatcagcatt tgcaactaca
    18961 ggacaagaat cgctaatatt aatatctcaa gtttgtgcta atttagcgca aggtggcgca
    19021 acattagctg tagcattaag aagcaaagat agtaagatga aacagttagc atcagctgca
    19081 tggatatcag ctatcatggg tataactgaa ccagctcttt atggagttac attgaaactt
    19141 aaaaaacctg ctatgttagc atctatagga gcaggaatcg cagggttatt tgcaggaatt
    19201 actcatgtga ctttatatgt tccacaaaat agtataatgg cagtacttgg attatcaggg
    19261 gaaaagggga tttcaaatat agtaaatgga cttatagtta tatttatagc attggttgct
    19321 ccatttatta tgacatatgt ttttggagtt gaaaaaaatg ttaaataaag tacagataat
    19381 gtagtagagg aagtataata ttaactattt acattaatag aatttttatt attattgact
    19441 ataacttgtt ttgtatttat attgatgcaa agaaataagt taatattaat aagaataatt
    19501 attagaactg acaaaaagca acataaaaga ttgatgaagt atatggaaat aaaaagttga
    19561 caaaatatgt ctgatgtttt atcatattaa ttggaattag gtagtaattg tttaagtaca
    19621 agctaagctt attcattaat aatagcagta tcctaggaag gagtatgccc attattatgg
    19681 ataagctttt tttatgctta aaaggaggat aatcatgata attaataaga tattaaacaa
    19741 taatgttgta atcacattag atgataatga tgaagaagtt atcgttatgg gtaaaggaat
    19801 tggttatcaa aaatcaaaag gcaatctaat agataaaact aaagtaaata aagtatttag
    19861 aatatcaaat aaagaaatat caaacaaact acaagaatta ctcaataata ttcctataga
    19921 acacatgaaa ttatcaagtg aaattataga atacgcacaa ataaagttaa acaagaagct
    19981 aaatgaaagt atttacatat ccttaagtga ccatacatat tctgcaattc aaaggatgaa
    20041 agaaggaata aatgtaaaaa atgcaatcct ttgggaaatt aaaagatttt ataaagaaga
    20101 gtttgaaatt ggaatgaaag cactagatat tattgaaaac aaaacaggta taaaattacc
    20161 agaagatgag gctggattta tagcctttca tatagtgaat gctcagttaa gtgaaggtca
    20221 tactttggca tctgatatta cgaagttaat acaagaagta cttagcatag ttaggtatca
    20281 ttttaggata gaatttcatg aagaatcagt gttttattat agatttataa tgcatttaaa
    20341 gttttttgcg caaagattgc ttttagatag tgcacatgaa ggtgagacag acaaagaatt
    20401 actaagcatt attaaatcta aatataataa agagtttgaa tgtgtagtta aaattaaaaa
    20461 tttcattaaa aaacaatata actacatatt aacagatgat gagcttatat atttaacaat
    20521 acatttagct aaagtagtta aagactcaaa tatgtaagtg aaataatttt tcagtagtat
    20581 attaagaatg attgaaagga gtgatactaa caaaattata cttatggttg gatggtaaca
    20641 ttgagttggc caaaccttca aatttcaaaa aattaattta aacgaaatat ggaggagaaa
    20701 aatgggaaaa tacaaacaat tagcacaaga gatagtaaac aatgttggtg gaaaagaaaa
    20761 tattagtggg ttagtacact gtattacaag attacgtttt actttaaaag atgaaagtat
    20821 agcaaatgac aatgtattaa aaaacatgga aggtattgtt actgtaatga aaagtggagg
    20881 acaatatcaa gtagttatag gaaatcatgt tgaagctgtc tataaagatg tagttgaaat
    20941 aattggttta gatgattcaa gtacaagttc agaaactaaa aaatcaggta atatactaga
    21001 taaaggtata gatataatat caggaatttt tcagccagtg ttaggaataa tggcagcttg
    21061 tggtatgttg aaaggattta atgcattatt tgtagcaatg ggattatata gtgatgtatc
    21121 tggtggttat atggtaataa atgcagcagg agatgcatta tttacattcc ttcctttgtt
    21181 cttaggatat acttcagcta agaagtttgg attaaaacca atgttgggat tagcactagg
    21241 agctgcagta tgttatcctg ctatacaagg tagcagtata tcagcaggag cagatgtgat
    21301 gtacacttta tttaaaggaa caatgtttgc atcaccagta tatattgatt tctttggact
    21361 accaattgta tcaattgatt atactggaac agtcgtacca attatattta tagtgtattt
    21421 tgcttctagg tgtgaaaaat tatttaataa gtgtgttcca aatttagtta agtttttctt
    21481 tgtgccaatg cttacattat taataacagt gcctgttgca cttatagtta taggccctat
    21541 agcaacattt ggttctacac ttatttcaca aattgtaatt tcaataagag attttagtcc
    21601 actattggca ggtgctatag taggatttac ttggcaaatt cttgttattt ttggtatgca
    21661 ctggggattt attcctgtat atataaacaa tgttatgact ttaggatatg ataatgttat
    21721 gatgccattc tttgcctgta catttgcaag ttcggcagtt gtgttggcaa tatttttcaa
    21781 gactaaagat aaaaaactaa aagagatggc aataccaaac tttatatctg gtatatttgg
    21841 agttacagag ccagcaattt atggaatatt attgccttta aagaaaccat ttataataag
    21901 ttgtattgct agtggtattg gaggcgcttt ctatggattc tttaatctta gaaaatttat
    21961 aactggagga atgggaatat ttgaacttcc agcaatgatt gaaccagatg ggggaatggg
    22021 taacttaata gtagctcttt ctggtatagc tatatcaatg gtcgtagctt ttgtattaac
    22081 tatgatttta tataaagatg agaaagtaga agagtctaaa aaagtaagaa ataagggtaa
    22141 agaagaaata aaagaagtca agtctactaa attagaaaga gaaatagtaa caagtcctat
    22201 aaaaggtgaa gtattaaaat taagtgatat agaggatgca gcatttgcat ctggtgtact
    22261 aggtcaaggg gtggcaataa tacctagtga tggaaaagta gttgctccag tagatggggt
    22321 agttacaacc ttatttccta gtctacatgc tatagggatt ctttctgata caggtgttga
    22381 agtgcttatt catattggat taaatactat tcaattagag ggaagaggtt ttgaggcatg
    22441 tataaaacaa ggtgatagaa ttacaaaagg tcaaactata ttaaactttg atgtttatgc
    22501 aataaaagag ttggggtata gtactgtaac tccaattgtt attacaaatt catcacaatt
    22561 tttagatgta gtagaaacag aaagtaaaaa tattgaatta gaagacaatc taattacagt
    22621 cttattttag taaagattga ggtattagca taatctagtg ctttggttat aattttagga
    22681 ggctttgact atgagtttta agaatgattt tttatgggga ggagctactg cggccaatca
    22741 atgtgaaggt ggatacaatg aagacgaaag aggattggca aatgtagatg tatgtccaac
    22801 aggaaaagat agaacagcag ttattacagg gaaactaaaa atgtttgatt ttgatgatga
    22861 gcattattat ccagctaaaa ctggtattga tatgtatcat aattataaag aagatataaa
    22921 gttatttgct gaaatgggat ttaaagtata tagaatgagc attgcatgga gtcgaatatt
    22981 tccaaaaggt gatgaagaaa caccaaatga aaagggactt caattttatg aaaatatatt
    23041 taaggagtgt aaaaaatata aaatagagcc tttagtcact attacacact ttgattgtcc
    23101 aatgcattta gttaaaaagt atggtgcatg gcgtaatcga aaaatgatag gtttctatga
    23161 gaaattatgc aatgttatct ttagaagata taaaggatta gtgaagtact ggctaacttt
    23221 caatgaaata aatatgattt tacatgctcc atttatgggt gctggtattt gctttgaaga
    23281 aggtgaagat gttgaaaaga taaaatatca agctgcccat catgagcttg tagctagtgc
    23341 aattgcaact aaaatagcac atgaagttga ttcaaataac ttaattggat gtatgtttgc
    23401 agctggaagt gtatatcctt atagttgtaa tcctaatgat gtatgggaag ctacaaagtt
    23461 agatagagaa aattacttct ttgtggatgt acaatctaaa ggtaaatatc ctaactatgc
    23521 gttaaagtat atggagcaaa aaggtattac accagaaatg gaacctggtg atattgaatt
    23581 attgaataaa tatacagtag attttatttc attttcatat tacaatagcc gttgtgttag
    23641 aaccgatgaa aatgcagatg atatggctga aggaaacata tttacatctg ctaaaaatcc
    23701 atatctttca tacagtcaat ggggatggcc aattgaccca ttaggtttga gaattacact
    23761 aaatcatgta tatgatagat atgaaaagcc actatttatt gtagaaaatg gattaggtgc
    23821 taaagatata gctgatgaaa atggttatgt agaagatgat tacagaattg attatctaag
    23881 agaacatata aaagctatgc atgatgcagt ttctatagat ggggtcgatt tattaggtta
    23941 tactacatgg gcacctattg atttagtaag tgctggtaca ggtgaaatgg aaaagcgtta
    24001 tggatttata tatgtagata gagacaatag tggtaatgga agtctaagaa gaatgaaaaa
    24061 gaaaagtttt gaatggtata agaaagtaat tgcttcaaat ggtgaagatt tatattagac
    24121 aagaaatcta tgttaaataa gaaatctata ttagacaaga aatctatgtt aaataagaac
    24181 tttgtattaa ataagaagag tataaaaatt aataataagt ataattttta acttttttaa
    24241 ctatagaaaa tcaatataga attattttac attgtattga tgcaatagta tgattcacaa
    24301 ataaaatttt agaaaataaa attgttaaga agtatgggaa gtaagtgcaa tttactttgt
    24361 aataaaagat gaaaaaggca agcttaggtt ttaagcttgt cttttttatt atgtataaac
    24421 tatgatattt taaaattcta tgaaaaattt atttaataga gaaattaaaa atttaatggt
    24481 aatcaaattc tttaccaatg aatataaaaa atgtctacgt gttataatag tttaaactaa
    24541 taaacgaaag gtagatgaat gaatatgaaa taccaacata taatagaagt tgacaaagaa
    24601 ctttgcattg gatgtgggct ttgtaaaaat gattgtccag taaacaatat aattatagaa
    24661 aataaaaagt cagtaattaa aaaacaagat tgtctaatgt gtggacattg tgcagcaata
    24721 tgtcccacaa aagctattac cttgacagga tttgatgaac ctcctataga acttacaaat
    24781 aagccaaagc tagattcaga tgaattacta atggcaatta aatctagaag aagtatacgt
    24841 aaatttaaag ataaagaagt ttcttcagaa attataaagc aaattattga ggcaggtagg
    24901 tataccccta gtgctaaaaa ttcacaagat gtatcatata ttgttttaga caataaaaag
    24961 tcaatttatg aaaacgaagc agtgaaattt tttagaaaaa taaaacctat tgcaaatata
    25021 gctattaaat attcaaaaga agttgagatt gacgataact ttttctttaa gcatgctcct
    25081 attgcaataa tgataattac aaaagataag attagtggtt cacttgcagc atcaaatatg
    25141 gcattaatgg cagagtctta tggtctaggt gttttattta gtggatattt ttcagatgta
    25201 gcaaataatt cgccaaaatt aaaaaagctt ttaggtctaa aacgtagtaa tcatgttctt
    25261 acaacattag taattggata cccagatgtt aaatatagac gtactgcaca gaaagaagta
    25321 gcaactgtta gatacttata agagagggtg gattttatgg aaaaagaatg tcaacaaaga
    25381 atgcaagaaa taacgtctaa atttaaagaa tgtcgcaaag ctatctctgc tatgggtgat
    25441 gagactagac aattgatttt aattgctctt ttagagagtg attttaatgg tatacgagtg
    25501 ggagaaatca caaaaaaaac acatctttct cgtcctgctg tatctcatca tcttaagata
    25561 ttaaaagaag cagagattgt taatttgcgt agagaaggaa cgaaaaatta ttattatctt
    25621 gattcaaaaa aatcacaatg gaagtcacta acagagctaa taaatcttat atttgttgga
    25681 atacaacata ttagtgaaaa tgatataaga aaaggtgatt aaattatact tgatttaata
    25741 caaaaagatt gatttaggaa ctaaaaaatt cactttgaga cactatcttt tgaactatac
    25801 ccattttagg agacggttaa aatatgtaaa actgtataaa acaatgggta tagttcattt
    25861 tataatggtt taaatattaa gaatatatta ttctaaatag tatctttctc tgttttgagt
    25921 tttattttta tactcaatag cttcagtggg gcaaccacaa atacatgcca tacaatgagt
    25981 acagttattt ttccaaactg gttttttgtt ttttaaatta atattattta aaggacatag
    26041 ctctacacat tttccacagc caatgcattt atcagtataa tgaaatcctt tggcactaat
    26101 aattgtctta taaaaaactg tatttattat accactttta attgtacctc ctaagccact
    26161 aggagtaata gtttcaaatt cattttcatt gcgtatatca gaaataatcc tatatatttg
    26221 tttatctgct tctttaatca tttgttttga tgtttctttg tctgttgtag aaaataaagc
    26281 tatataattc tctggcattt taatttctgc catacctttt aattgccatt ccttgtattt
    26341 acatagtttt tgaatatagt ttatggattt tgctgtatct ccaccacaag tcattacaaa
    26401 gtaaactctg ttgcttccta gaaattttgt ttcttttata aaatccgtaa ctactattgg
    26461 caatctccat gcataagtag gacatacaaa tatgaatggt ttatctgctg atattaattc
    26521 atctgttttt tcctctttta taagttgatt tagtgatatc agttcatcat ttaattcttg
    26581 tgcaatttta cgagccacat atcggctatt accagttccg ctaaaatata aaatcataat
    26641 aatatctccc taactatttt ttatttatta tagtaattta ttgatatatt ctattcaaat
    26701 gtatgtaaaa tttagtagtt aagaaaatta taacacatta gtaattttta tacttattgg
    26761 taaagaattt gattaccatt aaatttagca acaagacatc aactggactt gtatatgaga
    26821 agggataata atttgcttat gaaagattat aaatataaag gttattttaa aggtgtagaa
    26881 atatttgata atgtaaatga ccttaactta aatactttaa caacagttag tagatttgtg
    26941 cttgtttttt tgtttacgat aacttaagga aatatgttac ctgaataata aatttatata
    27001 ataaaagtat ataaatatat atatgaatgt taagattagt tgatagaatt taaataataa
    27061 atccaatatt ataaacatag gaggtatgaa aaatatgatt aatgagaatt taaaaagtct
    27121 aagaaaaatc aaccagtata cacaagaaga attagctgaa aaacttaatg tttctagaca
    27181 atcaatagca aaatgggaaa gtggagaatc tattcctgat atatgtagtt gcattaaact
    27241 tgcaaaattg tacaatgtca aactagatga cttagttaat cattcagaag aaaaaacagg
    27301 tattatagta cctcctaaag gaaagttttt ctttggagca gttgtagttg gtgaacgtgg
    27361 tcagattgtc attccaaaag aagcaagaga agtatttaat ataaatgcag gagataaatt
    27421 attggtactt ggagatgagg aaagaggatt aggaattgtt catcaacgtg atttgataaa
    27481 ttttataggt catgtgggag tagtacgtaa agaagaatga tttaaaaaaa ttataaaagg
    27541 aggtatgaaa tatgagtaaa atagtatttt ttagcattcc tgcatatggt catactaatc
    27601 ctactattga agtagttcgt gaattagtgg atagaggaaa tgaggtattg tattattctt
    27661 ttaatgaatt taaagataaa attgaaggtg ctggagcaaa atttatttgt tgtgataaat
    27721 accttccaga attactccct ggagatgaga aaaaaattgg taaggatttt cctggattaa
    27781 ttgagatgat agtagatact actatttctt tagatgaaaa agtttgtaga gaacttaaag
    27841 attttaatcc agattgcatt gtatcagatt cattaagttt ttggggaaag ttatttgcac
    27901 aaaaacttaa tattccatat gtatgctcta caacaacttt tgcatttaat aaacatacag
    27961 caaagttgat gaaacaaaac tttatagaaa ttatacgtat gatttttggt gtaagacgca
    28021 ttaataaaaa aataaagctt ttacaaagaa aagggtatga agtaaaaaat tttatttcta
    28081 tagtttctaa tgataatgat acggatacca ttgtatatac atcaaaagaa tttcaaccaa
    28141 tggtagaaac cttctctagt aagtattcat ttgtaggtcc atcagtatca aagttgatta
    28201 ttgaacctaa agagagaaaa agaaagctaa tatatatttc gcttggtaca attaataata
    28261 aaaatgttag cttttataaa aactgtataa gtgcatttaa agattctgat gttgatgtga
    28321 ttatgtctgt tggtagaagt actgatatta aaagtttagg aaatatacca aataattttg
    28381 aggtcaaaaa tagtgttgaa cagattgcta tacttcaaaa aactgatgtg tttgttactc
    28441 attctgggat gaatagtgtt aatgaaagtt tatattatgg agtacctatg gtattatttc
    28501 ctcaacattc ggagcagaga atggtagcta agagagttgt tcatttggga gcaggaatta
    28561 tgttaaaaga ggataagtca gaaagtatta aaaaagcagt atttcaagtt ataaatgaca
    28621 atgaatataa agaaaatgca actaaactat caaaaagctt ttataatgca ggaggctcaa
    28681 aaaaagcagc agatgtaata ttacagatta tatgtaatag tagacaagtt gacatataat
    28741 agatttgtta aaaatatata tacatacatg taatttaatc aaagtactga cagtggggtc
    28801 ttttaaccta ccagttgggg catatatatt gatgctaaat atgagggatg ttatatttta
    28861 ataaaaaaga taacatcctt tttttagtgc attatatcga gttttaaata ataaatgaag
    28921 gaggattaat atgaaaaaat ataaagttat ttttatagta ttaattatag gtattataag
    28981 tgtaacaggg tttgttgtat ataaaaactg cattaaacca tggaaagatg ctgaaacata
    29041 tattgataag tatatggtaa aacaagatat ttcaaaagat aatataaaaa gtattacaaa
    29101 agaaaaagcc aagaaggcaa gttacgaagg aatactatac aaagtgtatt acaaagatga
    29161 ccctcgatat gagtatcaat atttttatag tgataattat tatgctctat atgtaaataa
    29221 agtaatgtta caaatttatg atatggaaaa taataataaa cttttaagaa gtaatgagga
    29281 attaaaaagt gtaaagtatc cactaatata tcttaaatat aagccagata aatgatttat
    29341 gacaaaatag aatgattttt attaaaattt atttaaaaat atcactagag cgtttaaata
    29401 aaactaaatt taagtattta tatattagta tatcttacta gtgtttaaac aaaattaaat
    29461 ttaagtattt atata
//
LOCUS       DAEMGG010000044        28891 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEMGG010000044 DAEMGG010000000
VERSION     DAEMGG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.044"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(345..1436)
                     /locus_tag="KPK24_001775"
     CDS             complement(345..1436)
                     /locus_tag="KPK24_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299449.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTLMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQ
                     EMTVMKEDMSEVKQEINVLKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDMSEVRQEI
                     NTMKEDMSEVRQEMTVMKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1666..2751)
                     /locus_tag="KPK24_001776"
     CDS             complement(1666..2751)
                     /locus_tag="KPK24_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG2299450.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFDPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2787..4100)
                     /locus_tag="KPK24_001777"
     CDS             complement(2787..4100)
                     /locus_tag="KPK24_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2299451.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4101..6392)
                     /locus_tag="KPK24_001778"
     CDS             complement(4101..6392)
                     /locus_tag="KPK24_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2299452.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGSDKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEQFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6382..7176)
                     /locus_tag="KPK24_001779"
     CDS             complement(6382..7176)
                     /locus_tag="KPK24_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG2299453.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7215..7661)
                     /locus_tag="KPK24_001780"
     CDS             complement(7215..7661)
                     /locus_tag="KPK24_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG2299454.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7733..9139)
                     /locus_tag="KPK24_001781"
     CDS             complement(7733..9139)
                     /locus_tag="KPK24_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2299455.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9157..10527)
                     /gene="hydA"
                     /locus_tag="KPK24_001782"
     CDS             complement(9157..10527)
                     /gene="hydA"
                     /locus_tag="KPK24_001782"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG2299456.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLETKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10660..11847)
                     /locus_tag="KPK24_001783"
     CDS             complement(10660..11847)
                     /locus_tag="KPK24_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG2299457.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(11974..13188)
                     /gene="dpaL"
                     /locus_tag="KPK24_001784"
     CDS             complement(11974..13188)
                     /gene="dpaL"
                     /locus_tag="KPK24_001784"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG2299458.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(13551..14222)
                     /locus_tag="KPK24_001785"
     CDS             complement(13551..14222)
                     /locus_tag="KPK24_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2299459.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(14305..16521)
                     /locus_tag="KPK24_001786"
     CDS             complement(14305..16521)
                     /locus_tag="KPK24_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2299460.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(16524..16994)
                     /locus_tag="KPK24_001787"
     CDS             complement(16524..16994)
                     /locus_tag="KPK24_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG2299461.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(17056..18450)
                     /locus_tag="KPK24_001788"
     CDS             complement(17056..18450)
                     /locus_tag="KPK24_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2299462.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(18523..19884)
                     /locus_tag="KPK24_001789"
     CDS             complement(18523..19884)
                     /locus_tag="KPK24_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG2299463.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(20181..21503)
                     /locus_tag="KPK24_001790"
     CDS             complement(20181..21503)
                     /locus_tag="KPK24_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG2299464.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            complement(21859..22899)
                     /locus_tag="KPK24_001791"
     CDS             complement(21859..22899)
                     /locus_tag="KPK24_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2299465.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            complement(23226..24494)
                     /locus_tag="KPK24_001792"
     CDS             complement(23226..24494)
                     /locus_tag="KPK24_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG2299466.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIHIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
     gene            complement(24487..26421)
                     /locus_tag="KPK24_001793"
     CDS             complement(24487..26421)
                     /locus_tag="KPK24_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2299467.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLEIIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            complement(26450..27172)
                     /locus_tag="KPK24_001794"
     CDS             complement(26450..27172)
                     /locus_tag="KPK24_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG2299468.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            complement(27222..28208)
                     /locus_tag="KPK24_001795"
     CDS             complement(27222..28208)
                     /locus_tag="KPK24_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG2299469.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSGKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            complement(28265..28765)
                     /locus_tag="KPK24_001796"
     CDS             complement(28265..28765)
                     /locus_tag="KPK24_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG2299470.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
BASE COUNT         8813 a         5648 c         2973 g        11457 t
ORIGIN      
        1 tttttatagt aaaaatttta ttcatattac tatttataat ttttatagag caattttagt
       61 gccaatattt gcatacattt gtatagtata aaatttttat gttctcattg ataaaatttt
      121 acttatttca agcgttctat tagattttat catctaaatt ttaggtttta tttttataaa
      181 ttaattgtat ttacataaaa aaaaagacct aaaaacaaat ttttaacggt cttttctcaa
      241 ttttttattt tcatttctaa aatttataat aaatagtaag aacactataa attcttttat
      301 ataaaattct tagtttgtgg atgataaatt agtgaattaa aatactactt tactgatttt
      361 agtttgacaa tatcactcca atttttacta gtggctaact ctactaaatt taagtctctt
      421 cttatttcag ctatatcctc ttttatagaa ttagtgtcac cttttatata tgctatgtta
      481 tgttctatat tatcatgagt agctttattt atttcagaat tatgtaataa acttcctaaa
      541 atattattct gttctatctg tatctctttc attgatttat cttcacattc tagactatca
      601 atcttaattt caattccata taatttttca tttgctatgg atacctcgtc cttaattcca
      661 ttcatatctt cattaatgtt atttatttta ttttgcataa agtttacttc tgatttaact
      721 tcggaaacat cttctttcat tacagttatt tcttgtctta cttctgacat gtcttttttc
      781 attacattta tctcttgttt tacctctgat gtatcttctt tcattacagt catttcttgt
      841 cttacttccg acatatcttc tttcattata tttatctctt gtcttacttc cgacatatct
      901 tctttcatta cagtcatttc ttgtcttact tccgacatat cctctttcat tgtatttatc
      961 tcttgtctta cctcggacat atcctctttc attatattta tctcttgtct tacctcggac
     1021 atatcttctt tcattacagt catttcctgt tttacttccg acatatcttc ttttaataca
     1081 tttatctcct gttttacttc cgacatatct tctttcatta cagtcatttc ttgtcttact
     1141 tcggatatat cctctttcat tatatttatc tcttgtctta cctccgacat atcttctttc
     1201 attacagtca tttcttgttt tacttccgac atatctcctt ttaatacatt tatttcctgt
     1261 tttacttccg acatatcttc tttcattaaa gtcatttctt gttttacttc cgacatatct
     1321 tcttttaaca catttatttc ttgttttact tccgacatat cattttttat gctaactaca
     1381 tcttcattta tagtatataa taactctaaa atcttctctt gcacttgcat caccatccct
     1441 attaattatt ataacatttt acacaaatgc tatacaatgt taacatatat atcagaatta
     1501 ttaaaatttt aattaaacaa aattgattca ctttattact tcaaaactaa ttaatatcta
     1561 aaataactac aaataaacaa aaataaggtc aacaacaaaa aaggcttatc tctttgagat
     1621 agcctttctt atttttattt aaaactttgt tttttataaa ataatctata cattcagctc
     1681 tgaacatgaa atttttcttc caagtgcttt tggtttacaa actgatatac aaagtccaca
     1741 atatctacac tcttcttcat tcaattccat cttatcattc aattttctcg cttgatatgc
     1801 acaaactctc tcgcacatct tacatttagt acatttttct tcatcataag taaactcaaa
     1861 gctaagtctt tcattaacat ctcccatatg attatgtact ataccacaag cattttctat
     1921 agtattatat cctaatctat taagattaga ttttaaatca tcatgtattt tagatattac
     1981 ttgtgcgcct tttaaaatta ctactgaaca tataccaaca aagtcagcac ctgccataat
     2041 catttctaag gcatcatctc cacttacaca tccacctaag ccaattattg gtatattaac
     2101 ttgttttttt atttcatata ttctctgtaa agttattggc ttgatagttt ctccacttaa
     2161 atatcctatt ccatttccac ctaataaagg ttgccctgtt tctatgtcta ttctaaatgc
     2221 tggtcctaca gagtcacaag cagttattgc atctgctcct gcttcttcta attttttagc
     2281 aaaatcgcct atctcatcta tcataggagg aagtttaact attacaggta tatttactct
     2341 ttcctttgca tctttaagca ttggtatcaa atctctatag tcataagata ctaattcaat
     2401 aaagtcagct cctgcatttg caactttctc tactaattcg ctactttctt caattgtatg
     2461 acctattgaa gctatacaga cagtcccatc tcttttcatt tgtggtattt caaaatctat
     2521 ccattgctct ggatcaaaat ctgtccattt ttcattattt aaaagagcat tagctgtatt
     2581 tctaaccata tgtggtgctg gatttatagc tctttctttt cttatagtct tagtcacaac
     2641 tgcccctgct cctgcatcac ttaatatctt gcatccagct gcactatatg ttaatggtcc
     2701 tgaacctatt ataagtggtg attttaattc tttacctaaa aaattataca ttatctgctt
     2761 ccccttttca tatatattta cattcgttaa aatttccaaa attcacaagc aacttctttc
     2821 atttctttaa caatagcttt tttatctaaa tcaactaact gtttttcttt taaaatatgt
     2881 ctaccatcta tgtatacatt tgatataaat tcttttttac cctgaactac tatctggtca
     2941 aatatattat caagagtcac aggagtttta aattcatctt ccataactat tatatcagcc
     3001 ttattaccaa ctgctatttt accagaattt tgaagtccta atacataagc accatgctct
     3061 gttgccattc taaatactaa atttgctggc attactgatg catcttcttc aactgattta
     3121 tgcattaaaa atgcacctct cataacttca aaaaaatcat ttatatatcc atcagtaccc
     3181 agtgccactt ttatgccttt tttaatcatt tttggtactg gagaaaaacc tccacctact
     3241 tcacagttgc taagaggcat atgaaccact tttacacctt tttcttttaa tatatctatt
     3301 tcttcatcat ttacttttac acactgagat gctaatacag tttcatccaa tatatctaaa
     3361 tcattatagt agtcaactgc ttttttgcca aaatgttttt cagcatagtt tggctcatat
     3421 atactctcac ataaatgaaa ttgcattggt gcatttaatt tttttgcatc ttctttagct
     3481 ttcttaataa atctatcaga acatgtgaag cttgtatgtg tacacattat tccatttatt
     3541 aatttactat tttctctact ccactttatt aaattagaat tttcatctaa acatctaagt
     3601 ccattttcaa agcttattct ttcacaactt tctaaggata aaatcgcttt catgcctatt
     3661 tcttctagta tttttccttg ctcaattaaa gttccttctt ctgtatttgg agcttctagg
     3721 gtatcacaaa aagctaccac accagattct ataagttcta ttgctgatgc tttagttgtt
     3781 gccttcactt ctttcaatcc aattctattt tctataaatg gccaccaata gtcatttaag
     3841 aaaccttcaa agtcggtaaa atgtacattt gccggtattc ccctagaaag aactccatat
     3901 tgatgcatat gaccattgac gaatcctggc attattatag catctgattt atctattatt
     3961 tcttcaatat ttttatattt tatttcaact tcttcatttg gcaatatatc ttttataatg
     4021 tcattttcta ctacaacagc agaatttgta tatatttcat cagcagaaga tattaagtat
     4081 ttacttttaa tagcaatcat ttatttacct ccctgattta attttttcat ggccatataa
     4141 actttctcag aagttatagg aagttctctt atacgaactc ctgtggcatt gtatattgca
     4201 tttgctattg ctggtgcagg tgtatgaata actatttctc ctaaagattt agctccatat
     4261 ggacctgttg gctcataatt atctacaaaa tcaacaatta tgtttcctat atcttttttt
     4321 gttggaactt tgtattgtaa tagattagaa gtttgaagct ttccatcaga accatgtcta
     4381 gggtcttcat acattgcaag ccctatacct tgagttacac caccttcaac ttgtattcta
     4441 gcaagtgcag gattcataac tgtaccacaa tctgtagaac aagcataatt tattacttta
     4501 aactcccctg ttgttttatc taactctatt tcagcaaatc ctgctaagaa tggtttagca
     4561 ctctcttgta taccataaga ttcagatgaa tataaaactt cttgatttcc accaccaaca
     4621 gattcttttc ctaattcact taacaaaaca aactgctcta catcttcact ataacaaact
     4681 ctgtcttcat atagtattaa atcatctatt ggtaaatcga gtttcttctg tgctactttt
     4741 aatatctttt gttttaaact ttcagcagat tttattgtag catttccagt tacataagta
     4801 gtacaagaag cgtatgcacc agtatcatat ggacacatat ctgtatctcc tgtataaacg
     4861 gaaattctat caatagatgt attcaatgct tttgcagcta tttgtgcaag tattgtgtca
     4921 gaaccagttc ctaagtctga agacccagaa aacaacttat aagttccatc ttcatccatc
     4981 ctcatattta ctattgccat gtctatgttg gctataccag aaccatgagt tcccacagac
     5041 attccaacag ccctcacctt attgttgcca atatccctaa cagggtactt ttcatcccat
     5101 ccaataagtt ttttacctct aattatacat tcatcaagtg cacaacttct tattggaaaa
     5161 ttcattattc cgccttcagt ttgttttttt ataatattct ttaactttat ttctgttggg
     5221 tccatattaa gtttatgcgc taattcatca attgcacaat caagtgcaaa tgtaccttgt
     5281 gttgctccat atcctcttaa tgctcctcct ggaactaaat tagtatatac agttctccca
     5341 tcaaatttta ttgcaggtac attattatat gttggaagta cattatgacc tgattcagag
     5401 caaactgaag gtccattatc accataagcg cctgtattat tcaatgcttt catatctata
     5461 gcttttatgt ttccttcttt atctgaccca atttttatat caaaaaacat ctgatgtcta
     5521 gtattagtca ttgcaaaagt ttcttctcta gtataaatta gttttgctgg tctttttgtc
     5581 atccaagtta caaatgatgc atatatctct gttacagcta tgttttttcc accaaaacct
     5641 ccacctattc ttggttttat tactctcact tttgaaagag gtattcccag tgctttagaa
     5701 acctgtcttc tcatatgata agctgattgg tttgcagaag ttactactaa tctgccattc
     5761 gtatctatat acgtataagc tctatgagtt tccatcatac aatgggcttg tgattgagtc
     5821 atataagaat gttctactat tacatcactt tctttaaact ctttttctac atccccttta
     5881 cccatttttt ctctcgatac aatatttttt gtagcatcca atccaaaatc aaatggacaa
     5941 aacaaatcat cttcttcatg gattaaaatt ttatttcctt ccgattctct tgcatccaat
     6001 ataggagtca ttacttcata ttcaaccttt ataagtttca tagctttaat agcagccctt
     6061 tcatcttcag cggctattat agcaacttca tctcctacat atctaactac gtcttcaagt
     6121 attaattggt catgaggtga tgcttctgga tatgactctc caaccattga atatctataa
     6181 tttggaacat ctttgtaagt gaatatgttt actacacctt ttacctttaa tgcatttttt
     6241 atatctatgt tttttatctt cgcaaaagca tgtggacttc taagaagctt aattgttaaa
     6301 acatctttat gaaaaattaa atcttctgta tatattggtt ttccagtaac aatggcttca
     6361 ctatctattt ttcttacctc tttatttacc atcacactca cctccaatac tattatacat
     6421 tctcactact aaggcttttg ccatatcttt tctgtattct ttacttcctc ttatattact
     6481 tccaaatgaa agttcttcac ttgatacttc accagctttt tctatatcat tttctaaact
     6541 taatatttca cttgctttat aggcaatttt tgctcttctt ggtctagcgc ctattgcaat
     6601 tttataaata cttccttctt ttaacattgc tccatttaat actgcaaaat cacctgtaca
     6661 ttttcttata gaatcaaata cacctatagc atctttttta ggtaatataa cttctattaa
     6721 tatatctttt tcaagctcac tttctaaaaa atcagctaag ttcataaccc cttttttata
     6781 taacttaacc tttgcatcta ccactagtaa tgaaggtatt aaatctgaaa atccatactt
     6841 agcaaatacg ctagctccta ctctagctcc acttctaaat tgaactccta ctatattaga
     6901 aacagcgttt gaaattaccc cattacagta atttttaata accttattta attctaaagt
     6961 tcttaaactt gtgtctgctc caattaatat gctatcatca gtttcattta tataatctaa
     7021 agctaagtct tttaaatcta ttgctgagcc aatattttta ctacctagtt ttaaaaatcc
     7081 acatcctcca agtattatgt tgttttttct tgatattagc agttcataag cttcatctaa
     7141 actttttgga actacatatt cctttatagt tatcatactc tctccccata tcaacttaat
     7201 tttcacttaa aagtctaagt gtttaaatat ttatgtattg ctcttaattg acctttatat
     7261 cctgtacatc tacaaaggtt accatttaca taatctataa cttcttcttc tgttggattt
     7321 tgaagctctc ttttcattgc caaaaccatc attataaatc caggagcaca aaatccacac
     7381 tggtcagccc cttcgtctgc aagaaaatct ccaaacttct ttgcttcatc caagacaccc
     7441 tcaagtgttg taatatgctg accgtctact ctagcagtaa gataattaca tgaaagcacg
     7501 gccttgtcat ttaccatcac tgtacataac ccacatgagc ctgtatcaca ccctttttta
     7561 acacttacat atccattttc tcttaatgta tctgctaaaa aatcatctgg tcttatactt
     7621 aattcttttt ctttattatt tatggtacat ttgataatca aataaatcac ctcaaaactc
     7681 atatttttaa gctaaagttt tactatactt ttctatgaat aaattacatt tattaataaa
     7741 ggctattcca tttatgacct tccattctat tttttatagt tcctcttaca caaagtctct
     7801 cagctgtatc ttgagcatct tttaatgctt tctcttcatt agctgtcaat atttttgaat
     7861 cttccattat tatatttcca tctactataa cactctctat atttttcata ctagaagaat
     7921 atactaatgt agataccgga ttatgcatag gtatcgcttt tggtgaaagc attggattaa
     7981 atattagtaa gtctgctttt ttacctatct ccaatgaacc tatctcatct tccattccaa
     8041 tagctcttgc tccatctata gttgccaact caagaacttt ttctgctgac attgctaatg
     8101 ggtcacactt attaacttta tgttgaagtg ctgttaattt cataagttca agcatatctt
     8161 gagagttatt actagcggct ccatctactc ctaaactaac tgttattcct tttttaagca
     8221 tttctggtac tggtgcaaca cctgaagata agtacatatt acttgctgta ttatgagaca
     8281 ctttcatatc gtatttttta gtaagttcca tatctttttc agttagatac acacaatgta
     8341 ccataagcac atttggtcca agtattccta atttttctaa aacatctatg tcatattgcc
     8401 catgcaattc ttttgctgct tctctatcaa aaggagtctc tgatatatgt actgtaaata
     8461 aagcatcatc atattctttt actactctcc atagcatttc tagcatttct tgagaatttg
     8521 accatattgc tgcaggagct actcctattt tgattcttcc attttctgta ttatggtgtt
     8581 tttcaaacaa tcttcttaca tctttttcaa ccgtttctac atcttgcatt attccaggat
     8641 gaactccaaa ttgagctcct gtattcatac atcctcttcc aagtattcct cttattccta
     8701 attctttata agcatctatt attccatcac aaagacctgg cttactatga ggatacatgt
     8761 agtcaaccat tgttgttata ccacttctaa gtccttcaat acatccaagc attgcagcat
     8821 cataagtatc ttttggctct aaatagttag ctgctggaaa tgtcatagtt tctaaccagt
     8881 ctttaagtac catatcatct cccaatcctt ttaataaagt ttggaataaa tgattatgtg
     8941 tatttataaa acctgggaat aaaactttac ctttagcatc tataattttt ttaacatcag
     9001 tatatttact ttctatctct ttagaatttc caatatcagc aatcttgtta tcttttacta
     9061 ctaatgcccc atcaaaaata acttctctct ctttattcac agttactatt atggcattct
     9121 taataagtat atccatcatt cttatttccc cccgcattat ttagtaattt ttcttttaat
     9181 atattgacct gttggatgtt ctttaacatt acaactttgt actacatgat ttcctctcaa
     9241 aaatacatct tcaactttac atttaacctt aaatccttca tatggagtat aatctacatt
     9301 ttgtttttga gttttataac taattgtttc ttctttattg atatttaaaa tgactatatc
     9361 tgcatcactt cctacagcta tctcaccttt tttgggatac attccaaata tttttgcagc
     9421 attcgtagaa gtaacttcca caaacttatt tacacttatt ctattttcta agaccccata
     9481 tgtatataaa agtgctaacc tatgttcaac accaggtgct ccatttggta tcttactaaa
     9541 atcatcaatt cccaagtctt tttgcccctt aaaattaaat gaacagtggt ctgtgccaac
     9601 tgtttgaata tctccactag acaatccttc ccaaaggtaa tttatgtctt ctttttttct
     9661 taaaggagga gacattacat acttagcacc ctcaaattta tccttattat ataaattgtc
     9721 ttctaataat agatactgtg ggcatgtttc tactatcata tctatgtttt tttctctagc
     9781 catttttaca gtttcaagtg actccctaca acttaagtgg actacatagg attttgaatt
     9841 agataattta gttatatcag caagtcttga aacagactct ttctcaacta tgttaggtct
     9901 tgtaagtgga tgatattttg tctctaaatt accattttgt atattttctt ctatcaatac
     9961 atctagtaaa tccccatttt cacaatgaaa tccaactata caacctaaat cccttgcttt
    10021 ctttaaaacc ctgtatattg ttccatcatc tactttcatg ccatcatatg ccatatacat
    10081 cttaaaagat actatcccat ccgaaataag tttttccata tgattaaatg tatctttatt
    10141 taaacatgta attgtcatat gaaaaccata atcacaataa caatttccac ttgctttttt
    10201 atgatatgct tctacacctt gcagtaggtt ttccccctca ttggcttctg caaaatctag
    10261 tattgtagta gttcctccag ctatagctgc tcttgttcct gtttcaaaat tatctgcagt
    10321 tgtaatagaa cctgcattca tatcaaaatg agtatgtgtg tctattccac caggaattac
    10381 aattttacct gtagcatcaa taaccttatc accatttatt tctaagttat ttcctatttc
    10441 aactatcttt tcatcttcta tccttaaatc acttatataa gtcttatcag aggtaattat
    10501 agttccacct cttagtattg ttcccatata tacctcctaa cttttaagct ttaaagtatt
    10561 ttctaagtat tgtaaaactt tatttaaaat tgtattttct aaaggtgcta tattcagcac
    10621 ctctagacta tatttaaata atttattgtg aactaaatat tattttccgt atacagattt
    10681 taatattcca tagtaacctt ctgttcctat aaataactgt tcttgctcta tatactcatc
    10741 tattgtgtga gcaagatttt ccttagaagg accaaatcct atagttttta ttccagcttc
    10801 acctgcatag tgacttccat tcgtacagaa tgagtattga gttatttctg aatctattcc
    10861 agcttctttt aatcccttat atgctgcttg aacaaattca tcttcttcgt catataacca
    10921 acctgggaag aatctttcac tttctatagt atttcctgta tagcaatcag ctttttcaac
    10981 cgcataagaa acttttgcat ttaattctgg gtcttctttc atcatttcat ctaataaagc
    11041 ttgtattgga gctaatacgc cttctcttgt ttctccaact aataatcttc tatcaaaagt
    11101 agctttgcaa taatctggca ctactgatgc tcctggatat ggagaagatt ttatatctgt
    11161 taaaactaat attccatcac ctaaaactgg atgagttgga ggtactaatt gttgtatctt
    11221 ttggattaca ttagccattt tataaactgc atttacacct ttttctggat tagcagagtg
    11281 agctggttta ccaaaagttt ctactactat ttctcctcta cctctctgac caatctttaa
    11341 gtttaactct gaagactcac ctattacaac atagtcaggt tgaacagcct tacttatctc
    11401 tctagccgaa acaccttcaa atatttcttc atgaacaaca cctgctacat atagttctcc
    11461 agcaaaatct ctattagtgt ctttagcaaa atacgctaca gcagacatca ttgcagtata
    11521 ttgacctttc atgtcagaag ttcctctacc atatatcttt ccatcaacta tttttccttc
    11581 aaaaggagga acactccatt tacttgcatc tggaactgga actgtatcta tatgtccatc
    11641 aaataatatt ttcttacctg gcttgttacc ttttatacat cctactatat ttccatatct
    11701 atctcttgac caactatcaa aacctaattt ttcaaaagac tcttctattg cttttacaac
    11761 accttcttca tttccagaag aactaggatt ttgaattaat ttttggcata tttcagttac
    11821 ttcttgtttt ctttgttcgt ttaacatttc attatccccc taaatttttt ataatgtatg
    11881 tattttatat acttatatat tttctaaatt ttagaactta tatatcttct aaatatgtaa
    11941 accaatatct aataattgca ttttagtaat tccttatata cttggatatt ctccatccca
    12001 aactatactt ctatatttgt cagggtcagt atctccttct gtgcttatta aaagtattct
    12061 tgaattttca tctaatttta aatcttttct taaatctgct aaatcttctc tttccatagc
    12121 tgctactaaa agaccagttg taactgctcc agattctcca gaaattacct tttcatcacc
    12181 atttaaagga ttacctaatg ttctcatacc tttagcagaa acccagtctg gtgctgaaac
    12241 aaatgctgct gcatgatttt ttaatacttc aaatcctata gtatttggtt ctccacaagc
    12301 aagccctgcc attatagttg gcatatctcc tccaacaaat cttggttttc catctccagc
    12361 ttctgctgat ttatagtaac aatcagcttc atcagcctct actactactg ttattgggca
    12421 ttcatcatat attgaagcaa catacccttg aactgcacca gcaagtgaac caactcctgc
    12481 ttgaacaaat acgtgtgttg gtctatctac tccatattct tttaattgct ctatagcttc
    12541 ttgagccata gttccatatc cttgcattat ccatgctggt atttcttcat atccgtccca
    12601 tgctgtatct tgaaccatta ctccatggtc atcagcttct gcataatcat tagctaatct
    12661 tactgcatca tcataattta agtctgttat actagcatct gcaccttctc ctttaatttt
    12721 atttaatcta aattcagaag aaccttttgg cattaataca acagattttt gacctaattt
    12781 attagctgtc caagcaactc ctctaccgtg atttccatct gtagctgtaa agaaagttat
    12841 ttctcctaat ttttctttta tttctttcga agtcaatttg tcatacccta actcagatat
    12901 atctgtatct aatctttgag ctaagtatct acccatagaa taagaaccac ctaatacttt
    12961 aaatgcattt aatccaaatc tataagattc atcttttaca tatactcctg caacacctaa
    13021 tttttttgct aaaccttcta ggtttgctaa tggagtttct ttatattgag gaaagctttt
    13081 gtgaaaattt ctaaccttag ttatttcttc ttcatttaaa aatttgatac aattttcctt
    13141 atcgcccttt ggcaaattat ttactttcca tttaatttct tttaacatct aaaatacctc
    13201 caaattattg ttaaattttt atactttaat atataagcaa ttaacatgcc aacactaaat
    13261 atatactaga tatttttcta tgactcttta gtatagaaat tgcaatgctt ttattatttt
    13321 ttcttttcta tctttagttc atacaatgta tgtagaattt aataactaaa aaaccttttc
    13381 tatcttctca ctttttaatt ctcattttta aaatttaaaa ataaatctca aaacactttg
    13441 tttctatatt ttttaatata aaatctataa aatctaaatt aaaacataaa atagtgatgc
    13501 aataatctat tacatcacta tttgtatata ttcaataata taataaatct ttagtggtct
    13561 ctaagaggtg atttttcatc ttttttaaca tctaattctt cttttacgac attccatttt
    13621 tcaagaccaa acttagaaac cataactgcc tctgctgcac cttttccaag tctttttact
    13681 gtgtccatac ctgtctcatc ctcttgggca ccttttgctt ttccatcttt actccatatt
    13741 gtaccaccta tatagcatcc acctaaacct ccagacacta acatttcatg cattaggaag
    13801 aaatttatta taggaagttt tgtcaattct tggccaccat atctagttcc accagtagtt
    13861 atagctgcac caaccttatt ttgaagtttt cctggatgaa ctaaatacat tggacgaagt
    13921 ctattaaata ctgctgtaag ttgagcagtt atattcatat cataaactgg agaacctact
    13981 ataattccat ctgcctccaa tattttttgc tctaattctt ggatatcatc ttttataata
    14041 cacatagttt cttttcttat acatgcatca caatgtacac aaggacttat ttttttccct
    14101 ctaactgacc aatactcagt ttctatacca ggtatagttt ctgcttcttt caaagcttct
    14161 aacactgcat attctgttgc tcctttacgt ggacttccac aaatacctaa tatctttgcc
    14221 ataataatat ctcctcacca aataaatcta tttttattat ctatttacaa atacaataaa
    14281 ttgttatttc tgctttaatt tctactattt taattttatt ggtaatgaat gtatctctac
    14341 atcagttgca ttatatactg catttgcaac agctggcgca actggtacaa cggcacattc
    14401 tccaatactc ttaccaccaa aaggcccagc tttttcataa ccatctacaa atgcaacttg
    14461 acattttggc atatctacag ttctaaataa tttatatgtt ttaaaatcag tagctttagg
    14521 tctaccttgt tcatcaaatg ccaattcttc actcaatgcg tatcctagac ccatatgaat
    14581 cccaccttct aattgtcctt ctaaatttag aggatttata actctaccaa catcatgtac
    14641 agctacaaaa tcaactactt ttatatcctt agtttcttta tctactaaaa cttcagcaaa
    14701 atgtacacca taagaagtac gtccagctgg tgaagaataa tcttcaacaa cagttaactc
    14761 tcttaatgat ttactttgac tatatactgc gacatcagaa agtgatgctt ttacattttc
    14821 atcaagttta ctaattacac atccattgtc tagatataaa tcttcttttg atgtttcaag
    14881 taatttttct gcttcttcta atataagatt tttcaatttc tctgctgtct ttttagctcc
    14941 tccaccttct acaaataccc ctctacttgc atagtcacca aggttgaatg tacatgcatc
    15001 cgtatcagtt tcaaatgtat caactttatc aggtgttatt cctaaaactt cagctaccat
    15061 cattgtttgc atagttacaa caccattacc catatcatga gctcctgtta gtagtgtcaa
    15121 cgtaccatct tcatttaatt taagagtcaa tgtaacaaca tctctatgtg caccaaaaac
    15181 tccattacca tgtgcaccta ttgccatacc tatacctctt aaatattttc cttcttcttt
    15241 tggaagtttt ttcttttcat cccacttaaa catcttctta ccttgctcta tacaatccaa
    15301 tactctagga tttccaagag aaccgtggaa gattatatcg acatcatctg gctctactgc
    15361 atttttattt tgaaaatcga ctaaatccat acctatttcc tttgctattt tcgcaaattg
    15421 agcttgttga gccataaata tttgaggtga cccatagcct ctcatagctc ctgctattgg
    15481 tgtatttgta tatacaggca tcccagtaaa ctttatattt ggtattttat aaactttaaa
    15541 tactttatga ctaagtgctc ctataacatt taaagcactt gatgcatatg ctcctgtatt
    15601 tgtatatatt tttatgtctt gtgctattac ttcaccatct ttatttacac cagtttttaa
    15661 ttttacaaaa gacccatgac gagttcttgt cgatatcata gattcttttc ttgtaagaac
    15721 cattttaact ggtcttcttg tcatttttga taatgcggct gctactggtt caattgtaag
    15781 ttctaatttt ccaccaaatg agcctccaat agcaggtctt ataactctta ctttatttaa
    15841 tgacatccca aaaatttgag atagtattat tctaaatcca aatgtatttt ggcaaggtgt
    15901 atgtacagtt aatttatcat tataatcata gtgtgcaatt acactatgag tctctattgc
    15961 actatgatgt attgctggag tagagtattc atcttcaaat atatagtcac attccttgaa
    16021 gcctttttct acatctcccc catttatgtt gttttgagaa actatatttg aatctccatg
    16081 tatgcagata gcatcttctt ttattgcatc ttgtatagta aatacagctg gcaattcttc
    16141 atattcaact tttataagtt ttacagcttt ctctgctatt tctggagtct ctgcagctac
    16201 tgctgcaaca cggtcaccaa caaatctaac tgtatctgaa aatataactt cattttcagg
    16261 tatattgtgc tcataaaatc ttaatgaacc attatatttt ctttgtgatg tattgagata
    16321 agtagctata gctctaacac ctactacttt ctcagcttca cttgtatcta tactttttat
    16381 ttttgcatgt gctacaggac ttaaaagcat cttagcgtaa agtacatttt gtggtttcat
    16441 atcagccacg tatttcattt gaccagtaac ttttaaagct gcatctttta gagggtatga
    16501 ttttcctatt accttttcca taattatcta ccccctaatc tagctgcaga taattttaca
    16561 gcttctataa tctttacata tcctgtgcat ctacagagat tttctttaaa agcttctcta
    16621 atttcatctt cattaggaga tgcatttcta tttaaaagag cttgtgttgt tataatcata
    16681 ccaggagtac aatatccaca ttgtacagct ccaacttcta taaaagcttc ttgtattgga
    16741 tgaagatttt ctccattagc aaaactttct atagtgtata taactttacc ttcgcagttt
    16801 tttgcaagaa gtttacaaga tttcatgtca actccatcta tcaaaacttt acatgctcca
    16861 caactgccac tattacaacc ttgtttcgct ccagttagat ttaatttttc acgaagtaca
    16921 tgcaaaagag tttcattctt ttcaacttct atttcataca tgtaatcatt aatatttaat
    16981 tttatttttt ccattttctc ctcctacaga taaactaatg ttgtaataga atattattac
    17041 aatcttattt taatattagt ataagctatt ccacttatgt ccttccattc tattagttac
    17101 attagctctc ttacataatt cttctgctgc atgttgtgca ttaagcaaca cttgttcttc
    17161 actttcaatt gtagttactt ttccattttc aagtattaca tttccatcaa ctaaaacact
    17221 ctctacattg tgcatactag ctgagtatac caaagttgat acaggattgt gaagaggtat
    17281 tgattttgga gaaagcattg agttaaatat tactaggtca gcttttttcc caatctccaa
    17341 agaacctatt tcatcttcca ttctaagagt tcttgctccc tcaattgtag ccatttcaag
    17401 aactttctct gcagaaattg ccagagggtc acaattattt actttgtgtt gtaaagctgt
    17461 taatttcata agctctacca tatcttgtgc gttattactt gctgcaccgt caagacctaa
    17521 actcacattt ataccttttt ttaacatttc tggcacaggt gcaacaccag aagcaagata
    17581 catattactt gctacattat gagaaacttt tatgtcatac tttttaagca tttccatgtc
    17641 ttcatcagtt atacatacac aatgtactgc tacaacttct ggacctacta taccccattt
    17701 ttcaagtaat tttatatcta tttctccatg tatatccttt gttgctgttc ttgcaaattc
    17761 tgtttcagat atatgaactg taaaatatga atcatattct ttaactatat tccaaagcat
    17821 cttacccatt tcttcagata tagcccacat tgaagaagga gctacacaga tttttattct
    17881 tccattttct gaattatgat atttttcgaa tacccttctc aaatcttttt caaccgtttc
    17941 cacatcttct ataagctctt tgtgaatacc taaatctata caacctctac ctacaacccc
    18001 tcttatacct aaatctttat atgcctttac tattccatct gtaagtcctg gtctattgtg
    18061 agtatgcata taatcaacca ttgtagttat accacttttt aaaccttcca tacatccaag
    18121 cattgcagca tcataagtat cttgttctgt taagaatttt gctgatggaa acatcattgt
    18181 atttaaccac tcatgaagtg ccatatcatc acctaaacct tttaataaca cttggaacag
    18241 gtgattatga gtatttataa agccagggaa tattactttt ccattagcat ctataacttt
    18301 tttagcatca agatacttag gctcaatatc aactgtattt cctatgtcca agattctgtc
    18361 accttgaata gctattgctc catcaaatat aacatctctg tctttattaa ctgtaacaat
    18421 tattgcattt tttattagta tatcaatcat cttaaatccc cctttgaata ctttcattac
    18481 aacattttac catatggttt tctcatatta tatctaatac atttatccaa tattttcctc
    18541 aagatttgaa gtaacgtttt cagcactttt acgtgacttt aatttaaata atgtttgaag
    18601 taatatagct attattgctc cagaaactat acttgaccct ccaaggacag ttccaattgt
    18661 ttctgggaat tggtctaaaa cttgtggagc agttgtaaga cccatacttg ttgctataga
    18721 tacacctgct ataagattgt tttcttcagt gaatccatcc atactcataa tagagaaacc
    18781 agacattgct attgatgaga aaactactaa agttgctcca ccaacaacac aagatggtat
    18841 agttgtcatt attgcaccca atgctggaga tatccctgct agtaataata caagtgcccc
    18901 tagtgctatt acaaatctac ttgtaacttt gttcatagat acaattactg tattttggct
    18961 aaatactcca gttggaagag ctgcaaatag tgatgaaatt atagaagtta tagaatttcc
    19021 taaaacaact gatgctaatt cttcgtcagt tacctctcta tccattgctc ctactgtagc
    19081 tattgtacag gcacccatca tatcagctat acctattatg taaataatag taaacattac
    19141 tattaactct ggtctaaatt ctaatccaaa agctgctggt gttggtaaag atactatagc
    19201 tgctccttga acggctgaga aatcaaccaa tcctaaaact aaagatatag cataacctac
    19261 taatattcct attaatattg aagaattttt tactaaacct ttaccgtatt tatttatcat
    19321 tattattaca aaagcaacta taactcctac tgtgaagttt attgggcttc caaatgtttg
    19381 tgtgccttct cctccagcta aattctttat agctgttgcg aatagtccaa gtcccataca
    19441 tgctattata gttccagaaa ctattggagt gaatatatgt ctaattttct taactccaaa
    19501 tcctacaaag aatacgatta ccccagcagc aatctgtgct ccaaatagag ctgctaaacc
    19561 atctttagct gctactgaaa gacaagctcc taaaaataca taactcattc ccatcattac
    19621 aggtaatctt gaacctaact taaatccttt aaataaaggt attggatata actgtaaaaa
    19681 cgttgctaaa cctgctgcta acatagaacc ctgaatcaac attgttgctt gcccctggtt
    19741 aagtcctacc acgtttgcta ttaatatcgc aggtaccata tttcctacta tcattgctaa
    19801 cagatgttgt aaggccaaag gtattgattc ctttagtggt ggtatcccat cgacatcata
    19861 ttttgaagta ttacgtgttt tcataaaata aatcctccca tacataaata tcttgactaa
    19921 atatttttat aattattttc tataaatatt tttatgacca tgaatttatt gctcaaccga
    19981 taaacacagc taaactgagc tatttttata aaaaattaac aaatttctaa atattccctc
    20041 cccgattttc aacttaaatg aacattaatt gcctggtata tatttatttg ttaaattttt
    20101 tattaaaatg aatagctatc aaaaataatc taacaatctc atactaaata atttaaacaa
    20161 ttcttttaaa ttattatatc ttaattagtc tcttccataa cagctctttc tttagcctca
    20221 tcctctagac ttttatttgg tattattaga tttaaaacga atgcaaccaa tgttgcaatt
    20281 actattggag attctccaaa tactaacttt acaaatgaag gaaatgcctc ttgggctgct
    20341 ggtactgatg ttattcccat gcctaaggca attgccaaac caacaatggt aatatttctt
    20401 gatgacaact cttctgtaat aattaacttt atacctgtca ttgttatcat actaaatata
    20461 gatattgtag ctcctcctaa aacagaatat ggtatagtag tcattaatgc accaaattta
    20521 ggcacaaacc ccgacaataa cataaatata ccagctattc caagcacaaa tctacttatt
    20581 actttgttca tagctaccat ccccacgttt tggctatatg atgatggtgg taaacctcca
    20641 aacaaagctc ctatcatagt acaaattcca cttcctatta ctccacttga taattcctta
    20701 tctgtgatat tcctatccat gcctcccatt gtagtagctg ataaatctcc aattgcttga
    20761 acagcattga ttatacttac aattactact gagattatta caggcatatc aaattccatc
    20821 ccaaattcaa gcggtttagg tatcgcaaac caagttgcct ctctaatagg gtcaaaattt
    20881 attataccca gaactaaaga tacacaatat ccaactataa ttcccaaaaa tattgctgcc
    20941 aattttgcat atccttttgc aaattgacta agacctataa ctaccactaa tgttatagcc
    21001 gctactgccc agttttgcat agaggcataa gtaggactat ttacaccacc tgccatataa
    21061 tttattgcta taggatacaa agataatcca attgtaaaaa ccactgttcc tgcaactata
    21121 ttcggaaaaa attttcttat cttttttatg aatatcccta ttaatactgt agccactccc
    21181 cctattaatt gtgcacccaa aatgcttgct aatccataat ttcctccgac tgctacaagt
    21241 gttggtacat aagtaaaacc aactccaaaa attataggca atcttgaacc aatatttcct
    21301 attggatata gttgtataag tgttgatata ccagctatta ttaatgcata ttgcactaaa
    21361 agtgttattt gtgtagggct aacacccaca gctcctccta cgactatagg tactgtaaca
    21421 gttccaacta tcattgctag tatatgttgc attgatagtg gcaaacactt taaaattggt
    21481 ggccttccat caatatcaaa catagaaaca ttcttttgat atttactcaa ggttatcact
    21541 cccaactatt ttttaatgac agttattatt atctcacaca acatcctttc tttttttatt
    21601 ttgtcatctc aacttaatat gaagcaatta taatgccaat tttttaacat aattgtttta
    21661 catatataat ttttattata tttgttagta tttgataatt tattaaagca tttatttttt
    21721 taaattcttt taaatttttt ttatttttgg ataataaaat gctattacac aaaaaaacac
    21781 ttagaaaata gttttccaag tgttttttat caattttata atttcaaatt ttagaattga
    21841 gaataaaaag cctaattatt atacatctat ttttctgtac aatgtagcta gtgatattcc
    21901 aagttttttg gcagccagtt ttttagactt agtatcattt ccgtaaaact ttaacacttt
    21961 ctttatatag gctctttcaa actcttcaag tgtaagtatg tcttgttcca tacttatctc
    22021 atagtcactt tcctcaagta actttatatt tatattattt aacttaaaat attcaagtat
    22081 ctttctattt attattcctt tatgtattat tccatcttcc tcaagaaggt ttaatgccag
    22141 ttctatagta ttttccaact ctctaatatt tcctggccaa ggatacttaa gcaacatttg
    22201 ttccacatct ggctctatat ggtacacgtt tttcttcatc tttctcgaat atttgtctat
    22261 aaaattgttt attaatattt gtatatcgtc tcctctttct ctaagtggag gcacttctaa
    22321 aggtattaca ttcaatctat agtataagtc ttctctaaat ttattttcct taaccaactc
    22381 aaataaatct ctattagtag ctgatatgat tctaacatct atatcaatta gttcatttga
    22441 acctattctc gtaaacttct tttcctgtaa taccctcaat aactttgatt gtagttgaat
    22501 tggcatatct cctatctcat ctaaaaatac aacccctcca ttggccaatt caaacttacc
    22561 aattttacct tttgaatctg ctccactaaa agctcctttt gtatatccaa aaagttcact
    22621 ttctagtaaa ggttctggta tagcagcaca gtttatagca acgaatgttt tattccctct
    22681 gtttcctgcg agatgaatgg ctcttgctat taattctttt ccagtgccac tttctccagt
    22741 tatagctaca gtagaatcac tatttccaat tttacaaatt ctattcttaa gagcacgcat
    22801 tacatcagac tctccaatta tggagtctac tgttgttgtt ccccaaatat tatatacttc
    22861 cattccacca tacattactg aatcactgtg tttgtccata aaatatcaac tccaaataag
    22921 tattctatat aataatcatt cacattatct atttaattat atcataacag aaaagcttag
    22981 ttataattta ataatattat tttaaaacaa aacttaataa aaagtatatc tacttagttt
    23041 taatttgata gatatacttt cttcatttta caaaaatcct ctattaaata agcaattaca
    23101 atgccatatt ttatatggaa ataattttta aaatttttaa tttaagttct tattaaattt
    23161 tttatactac tcgtaatttt tatacctata taaaattact tttataaatt ttaaaattct
    23221 tatttttaaa atttataatt aaaaattatc ttttttaaat cattttttgt gttttttata
    23281 tcatctaacc tcacagtttt tgcttctata aacttaccct taaatgattc tgataatttc
    23341 caggaactat aattcttatc tttttgaata caaggatata ataatataca tctttttgca
    23401 ttttcatatg ctgttatata tgcatacatc tggtatatat cacttgacct ataaaaaaca
    23461 tttgaatcta cttcaatagc tttccattta gtgtcaatta taatttcata ttcatttttt
    23521 aagtcatata aaactatatc cggtcttaaa ttaaagtttt tcttacctgt ttgttcattt
    23581 ttcaaaagaa atttactctt atcttgtata tatgtttctc taaatgaatt gtcccatata
    23641 cttttgatta aaattcctat atattcttca tacaaagtat ttatttcaaa taatatagaa
    23701 aatgcctctt gattattttg actattatcc atagacaagt ttaaaagtag aagtctagct
    23761 agtaaataac aatctttaaa tctatcattg tttttataaa atttataatc caacagtttt
    23821 tttctatcta tatgaattaa atctacattt tgaaaatagc ttaaaatttt tttaatcttg
    23881 ccttgaattg aattgtcatt tattctacat aaaatagata tacatgcttt tttaagaact
    23941 tgattcaaga aattattttc actatactca tcatattcac atcttatttt cattggagaa
    24001 atgccttttt cttttgcata tgtacttaat aatattttac ccctcataac atttaagttc
    24061 tctattttat ttatatattc aaaataaata ccttttttca tctgtgtttg catactctca
    24121 atgaaataca taacaaaaaa atttaagaga ttataatttt tcaaactcaa tcttattttt
    24181 tcattcattg taattgggat tttattacat atagaaagca tctgtaatag tatctctcta
    24241 tcttttacaa gattatcact taatgacaat ttaggaagta tttcaagtat tacattttca
    24301 aaacatatga tgcctacata gtttataaat ctaagttttt tgtatttaat atctattata
    24361 ttacttttta atacatcatt attgttctct agatacttaa gcaatttttc atattcaatt
    24421 tctgtaagct cattttcacc attacctatc cttatccatt cataggtttc cttaacatat
    24481 atatttttac tcatatatat tttttagctc ctcactagat atgttttgtt ttatacaata
    24541 gttaacttta ctctccatta ttgaggcatt attattttta aataactcag aagctataag
    24601 tttttcttta taaacaatgt atttatcttt ttcatttctt ccaattcctc ctaaaatcaa
    24661 acctactttt tctgaatcat aataaaaata ctcttgaaga agtggtatta ttttacttct
    24721 aaatactact actatatcat taatattttc tacagcaagt agataagctt gtccaataac
    24781 atggtctcta tcatataaaa actctatcct tttattgatt gtatatagca tttttctaat
    24841 atctatacca tctacaatag acaataaatc ataatctggc ataatttctt caaaattaaa
    24901 ccttcttctt aaagctatat ctaaaagtgc tattgattta tctgatgtgt tcatagttcc
    24961 aactatatac aaatttggag gtagagaaaa tttttccttt gaatatggta gtttgacaat
    25021 agtttgattt gatttagtaa gtcttttatc atcttccaat aatgttatta gctcaccaaa
    25081 tattttagat acattccctc tattaatttc atctattatt aaaacatagt tttctgattc
    25141 ttcaaaatta aaactctcac tatattctat attttctaat actaatttct ttttcctgtc
    25201 ttttataatt tctaaagtca agctatcttt ttccaactca aagctcatct ttttttcaaa
    25261 tttcaatgca ttataacatg cttctataga tatatccttt aatatcccat cttctggctt
    25321 aaaattgcct tcaccatcag acttaagacc ctctataaat tcctcatatc catatgattg
    25381 atgaaatgta caaaattcta cattgttttt ataatctaag taacctgtat agtatctgtt
    25441 tcttagactg gtttcactta ctagattgta aaccatctgt tttgtattgt atgttttacc
    25501 agttcctggt ggtccataaa gtatagtatt taatggcatt ttattagtaa atctttttac
    25561 atacttttca actatactat tattttcaaa tataacccat gcatttccgt ctatattctc
    25621 cttttcatac tctatccatc taacaggaaa aaaatatcca ccctccaaag cacttacttt
    25681 ctctttatcg aacccttcca taacttgaca tacgccaaat agactcttca ttgtatggtc
    25741 attttcacaa attactttct cgctattata caataaaata taatcatctg tctttatatt
    25801 ttttaggctt tctaattgtt ctagattaat gggctgaata aataattctt catcatctac
    25861 acacttatct acatttttat aataatagtt ttttaaatac catttattat tttcactttc
    25921 ttctattctt tttctcaata atttcttatc ttgtgactta ataatttcta aatatgagct
    25981 aatatcatta ttatttaatt tataaaattc acttgtatat actttacaat ttgaaactgt
    26041 atcaaaatta tgcatccatt ttactggtag tgtatgccct aaaatatgac ttaattcata
    26101 tccattttca aaatcttctt caactatgcc aatagacaca ttacatttaa ctttttcttc
    26161 tatattttga tatgctttat ataaaaaata atctcctttt ttaatcgaca taaacttttt
    26221 tataaaatct ttatcattac aattctcatt aatatacttt tctgattttt tccccatact
    26281 gaatactttt tttaaattta tttttatatc aaatccaagt gcaattatgc tttcttctct
    26341 aaaaatctgt ggtgtgtctg tttcctgata aaaaaattct tccattaacc aatactctgt
    26401 acctcttata ttttcagaca tatacactct ccttaatatc taaaatatac taattataca
    26461 ataagcctac tctaatattt ccatttaaat tttcctttat atataaagaa aatattactt
    26521 catagctttc ttctaccttg ctctgaatat tacttattaa atcattcaat tccattatag
    26581 aaaattctgg acttccttca acaaatacaa ttcctttctt acttataaat tcctcaccaa
    26641 tatattccat cctgtcaaat aaaatgtcag ctatctcgct atatgacttt gtatcttcaa
    26701 actcttcaaa agaatactta attcctttat ctcctacaat agcttctttt aaatcagtaa
    26761 tatctatatt tatcatgcaa gaatcagaaa tagactctac catgatattc attaaatcaa
    26821 caaatttaaa tatactatca ttattgattt taaactctct cccaagctct aaatcttctt
    26881 tattttcttt ctctgaacaa tccattccta tagatagaac tcttctttcg tttgacatat
    26941 atgaaacagc ttttataata tctctaatat gtttatcttc agatgaatat atcaaaaata
    27001 atatatctac accatctaaa agacttctaa catattcttt ttcaatctct tgatttatgt
    27061 ttattttttc tatatccata tttgctttta ttttttcttc tacttcattt attatattaa
    27121 taccttcagc tccaatacca attattttag cattcttatt aaaattttcc attataacgt
    27181 atatctcctt aataattatt taatttcaac atttctaaat attataccat attttcttca
    27241 taaaaatatc ttaactcatt ggccttatca taaagtagct tattcaaaaa ttttggctct
    27301 ataaccttag cattatttcc aataaaaaat aaaatcgaag cataacaatc tatatcttca
    27361 acctttatga atgtactaat tattccacta ccatcttcat ttagctccat atctaaattc
    27421 caagaactgt cctttatttt agaaacacct tctttagtta ataatacatt tagataaata
    27481 atttcaccaa attttttagt agttgaacta tttccatata aattgttata tacttcatgt
    27541 aaccattctt ccaagtttat atgtattttt ttactttctc ctgttctttt tatatcaatt
    27601 atattatcta tatgaactaa tattatttta ccacttttac aatcatatgc tggtgaaaac
    27661 catgctccct tatttaaata tatacctata gggcatacgt ttttttccaa aatctgatta
    27721 ccacacttat acttaatatt taaaattaga gattctgaag aagctctaaa aaactcagtt
    27781 acattactac tcttaaaatc tccatcatta gtatcattag ctacaaagcc aatgtatctt
    27841 ttaatatttt ctatacctac ttttatactg cttttacgtt cataacttac ttttctcatt
    27901 atagaattaa tttctatttc cattaactta taattataaa tctgaagtga cttaagtgca
    27961 aatagtataa tcataacttc tttttctgta aatataacag gaggtatata gctattattt
    28021 agcaccctat aacctccatt tctaccttgt tctgcttgaa ttggaagtcc cattttattt
    28081 aattctttta aatatcgttg tattgtttta gctgaaagat taaattcctc tgacaactct
    28141 tttattgtaa acttttgctt atcattaata tatatccaaa catcagtcaa tctttttacc
    28201 ttcgacaaat aaacacctct tttcactaat aatactctca attaattaaa ttactataca
    28261 ctatttaaaa tttaatatta atgacatttt tatatttttt cattaaaaaa cagaaaaatg
    28321 gtactcctac aattgttact actacaaatt ctccaaaacc tacctgtatc atagatagaa
    28381 ttaatggaag cccaaagaat acatttaaca ttagaccaac cacaatcgaa tttataacag
    28441 ttggccataa agaagctata actaaagtac ttttattctg tttacctaac tttctagtaa
    28501 tataaatcat acttgcactt ataaatgtgg ctaatgttcc aaaaattata tcaggtacac
    28561 catatggtcc tattacattt gctaaaaaac aacctaatgt aagacctact atatatcctt
    28621 tatctaaaaa tgccaaaagc atcataatct ctgctaccct aaattgtatt ggtccataac
    28681 ttataaatcc caatgataat gttaaaactg catagattgc tgtaattgct gcagttagtg
    28741 ctatattaat actatttttt ctcatatctg tctcctagta aaagttttat ttttaaaaaa
    28801 attaataaca taactttatt ttatacaaaa tctataaact taaacagtaa ttttataaaa
    28861 acaaattatt ttagcgaact attttcccaa t
//
LOCUS       DAEMGG010000045        27673 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEMGG010000045 DAEMGG010000000
VERSION     DAEMGG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.045"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(120..728)
                     /locus_tag="KPK24_001797"
     CDS             complement(120..728)
                     /locus_tag="KPK24_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG2299471.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(837..3575)
                     /locus_tag="KPK24_001798"
     CDS             complement(837..3575)
                     /locus_tag="KPK24_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG2299472.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINVCISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3723..4514)
                     /locus_tag="KPK24_001799"
     CDS             complement(3723..4514)
                     /locus_tag="KPK24_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2299473.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4550..5206)
                     /locus_tag="KPK24_001800"
     CDS             complement(4550..5206)
                     /locus_tag="KPK24_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2299474.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5199..6164)
                     /locus_tag="KPK24_001801"
     CDS             complement(5199..6164)
                     /locus_tag="KPK24_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG2299475.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKNLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISNKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6270..6376)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6666..7610)
                     /locus_tag="KPK24_001802"
     CDS             complement(6666..7610)
                     /locus_tag="KPK24_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG2299476.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSASIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATVIEPIELKHLIKSKVLELYNHHCI"
     gene            7721..8188
                     /locus_tag="KPK24_001803"
     CDS             7721..8188
                     /locus_tag="KPK24_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299477.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8358..8957)
                     /locus_tag="KPK24_001804"
     CDS             complement(8358..8957)
                     /locus_tag="KPK24_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299478.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            9203..9799
                     /locus_tag="KPK24_001805"
     CDS             9203..9799
                     /locus_tag="KPK24_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG2299479.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(9897..10856)
                     /locus_tag="KPK24_001806"
     CDS             complement(9897..10856)
                     /locus_tag="KPK24_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2299480.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(10890..11621)
                     /locus_tag="KPK24_001807"
     CDS             complement(10890..11621)
                     /locus_tag="KPK24_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299481.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(11614..12384)
                     /locus_tag="KPK24_001808"
     CDS             complement(11614..12384)
                     /locus_tag="KPK24_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2299482.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(12469..13179)
                     /locus_tag="KPK24_001809"
     CDS             complement(12469..13179)
                     /locus_tag="KPK24_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG2299483.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(13359..13511)
                     /locus_tag="KPK24_001810"
     CDS             complement(13359..13511)
                     /locus_tag="KPK24_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG2299484.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(13537..15693)
                     /gene="feoB"
                     /locus_tag="KPK24_001811"
     CDS             complement(13537..15693)
                     /gene="feoB"
                     /locus_tag="KPK24_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG2299485.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(15793..16014)
                     /locus_tag="KPK24_001812"
     CDS             complement(15793..16014)
                     /locus_tag="KPK24_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2299486.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(16027..16239)
                     /locus_tag="KPK24_001813"
     CDS             complement(16027..16239)
                     /locus_tag="KPK24_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2299487.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(16560..19457)
                     /locus_tag="KPK24_001814"
     CDS             complement(16560..19457)
                     /locus_tag="KPK24_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2299488.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLNSGYEYSVLYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKYKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSNLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKANMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSIESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLKKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(19784..20392)
                     /locus_tag="KPK24_001815"
     CDS             complement(19784..20392)
                     /locus_tag="KPK24_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG2299489.1"
                     /translation="MVDNLIKYIHKSYVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(20652..21197)
                     /locus_tag="KPK24_001816"
     CDS             complement(20652..21197)
                     /locus_tag="KPK24_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG2299490.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(21315..23138)
                     /locus_tag="KPK24_001817"
     CDS             complement(21315..23138)
                     /locus_tag="KPK24_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299491.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(23151..24926)
                     /locus_tag="KPK24_001818"
     CDS             complement(23151..24926)
                     /locus_tag="KPK24_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299492.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
     gene            25219..27447
                     /locus_tag="KPK24_001819"
     CDS             25219..27447
                     /locus_tag="KPK24_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG2299493.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         9266 a         4663 c         2825 g        10919 t
ORIGIN      
        1 attaaatatg tttgtaattc tataaagtta tttttatcaa actaaaaatt ctataaaaaa
       61 ataagataca tacctataaa ataccttcct atagatacat atcttaccta tataaaaact
      121 tattctatat aaaaacgcca taaaaaatct tttgcttctt ctgcataatc tatatttata
      181 cgtttactag tttttatatc taactcatct ttactaaata ctttttttcc tttatcatca
      241 taataaaaat cacttatata taattcttca cccattatac tcttactatt tagacttcta
      301 tctattttta aagctttgca aagttttcct ggtccgttag taatattttt tacttgatat
      361 ttactcaact cagtgtaagc tttgttgtat ctattcatag atatctcatc taaagaagtt
      421 ataggctcta cccctcttat taatacacac tctggcacat tttctatatt tgcagataaa
      481 ttcaagcaat tatacatacc ataaattaaa taaacatata tatgtccacc atctaaatat
      541 aatggcttag ttctatctgt ctttttattg ccatatacat gtgctccttt atcatttaca
      601 cccatgtatg cttctgtctc tatgattttg gtaacaatta ctttattttc atatttcctt
      661 attagatatt tacctaatat tgatttggct aaatcaattc cattttttct aaaaaaatcc
      721 ttttccaaaa tttcacctca taaaatttat ttaaaacgca tcaaatacaa ctaaagtaat
      781 aaataaaatt aactcttatt aatgtgatat atataaacaa taaatttaga catttattat
      841 ttatatatat caaaaaattc tatctcaaac ttttcaatat tatcattgta atttgaatta
      901 tcatatactt cttcaacaac ctctttaatt gggaatataa cttttacatt agttccttct
      961 cctaccttac tatctatgta aatcttccca ttttgaattt caatcaatga ttttgtaagg
     1021 cataaaccta taccacttcc aaattcattt gatataccac tgttttcaat ttgtttaaat
     1081 cgattaaata tatcatttaa tttttctttg tctatgccca ctccagtatc ttttataatt
     1141 atctctactg tttcgtcttt tttatatata cctacaagga tttctcctcc ttctaaagta
     1201 aattttatag catttgataa tatatttagt attattcttt ctatcgattc tgggtcaagc
     1261 cctaccataa cagtttcttc atctgtatca aaaattaaat ctattccttt agacttagca
     1321 taactactaa tagatgtaac tattctttct gttatgtcaa taatatcaaa attaactgct
     1381 ttaatcttat atatgtctga cttaacttga gaaatattta ttatattatc tattaatctc
     1441 aataatctaa acatattttg tttagttatt tttatatgtt cttttagtat atcctgaaaa
     1501 tcaattattt ttcctttttt ataaaggttt tcaataagtt gaactgaact atatatatta
     1561 tttaaaggtg tcttaagttc atgagatata ttagcataaa actccatctt catttggtca
     1621 tactgtcttt gttttattaa tgctttttta agtctttcag actctctttg cacacttaaa
     1681 tctttaataa ttctcactct acttcttttc tgtccaaagt ataatgttct tgatatcatt
     1741 tttacttctt taatgtgatt attttcattt actatatgga tactcttctc tctttctcct
     1801 atatttaaat tgtttatgta tttttttact aatatatttc tatatttttg tgttgtaact
     1861 cctaatacat cactagaaat tccgaacaac tctctaaagg ctctatttgt atatagtact
     1921 cttgatgtac ttaaatcctc aagaaaaata gcctctggta taacatccag taatttttca
     1981 tatattattt tttgttcatt tactgttcta agaatattta tctctttagt tatatctcta
     2041 aatgtaagca atttaaagag tttattatct gaccaatagt ctccaactct aacttcttca
     2101 acatgctctt caccttctga gtcagacatc acaaaattaa ctatacagtt tgctgtgtca
     2161 ttgtaactta ctatttcagc tttcttttct tgtgttagat ttaatctttt aaattcccta
     2221 ttgtcaaaga aatctatgtt aaaagtttca aaatattgtt ctaatgtcat atcgactaaa
     2281 tcacttgaaa cacatttaaa actcttttca tattttttat ttatacttat taccttattc
     2341 ttctttatgc caccttcagc acttcttaca agtataggag atggaatatt tagtataatg
     2401 ttttcatagt ctttatttat atttcttatt tcttcatcta ttttgatttc atttgtaata
     2461 tcatttaaac ttattaagta attgctttca tctttattat tacttaaact taacaaataa
     2521 attttatcat ttaactctac cagtttatca attatactta tatcgttatc aaatttcttc
     2581 aatttttcag caactttttc atcttccttt ataaaagtta atattttata ataattattc
     2641 atatcctttg aaaattcatc tttaaattta ttatttgtgt aacttatatt aaaatcatag
     2701 tctgttacta tccatacatt tggtattgta tccagagctt gtctaagtaa ttcttttctc
     2761 tggtctatgt agttttttaa tgttctttgt ataatcatat ccttttctat tgctaaattt
     2821 atctttccta attgctctga ctttttactt aattttttat tgattgaatc aagttcataa
     2881 tatggataca ctactctatt attaagactg tatacatata cataataagt atggttacat
     2941 aatagtaaat atattactcc tattaatcta tctgaatcta ctatattaat cttagaaatt
     3001 gctacaaaac atataatagt tgtaaaaaaa acaaaatatg ttaaatcttt tattaactta
     3061 tagtgctcaa tgttactatc aagtttattc aacaaatata gtaaattaaa catagatata
     3121 caaacattaa ttaatataat aatcatattc cagttaatat cactaaaaat atttcttata
     3181 aagtaatcta atatcacaaa agcactataa aaaattagta ttgggcttat tcttagtttt
     3241 tttactttag aaatggttaa gtatataaaa gtagacatat taattatgct taagttaagc
     3301 aatacaattt ggaatttttg aatttttaaa tttactactc ctaaaatact tacaaaaact
     3361 aatacaagac accctacttt caatatattt ataaattcat ctgttttaaa gctacttata
     3421 taaactatag aatatccaaa tatccaaagt aaaattattc tatacatatt tatagaaact
     3481 gtatacaaat ttatatttaa aagtgcaata accaaaaata acccattagc tagtatatag
     3541 accttattct tacttaaagt tcctttagat tccatattcc ccaccacttc ttcattttta
     3601 tatagtttta cctataaata tgttaccaca attcttccaa aaaatttgtc actttctagt
     3661 tataaaaaaa aatgagtaaa tatttttaat tttactcatt ttgtagattt tgtatattca
     3721 gtttaaaaag ctggaactat acttccttta tattcatctt gtataaattt tttaacttct
     3781 ttactattca tagcctctga taaagcctta atcttatcac tatctttatt attttctcta
     3841 caagctatta tatttacata tggagaatca cttgattcta ttactattgc atccttagta
     3901 ggatttaagt tagctgttaa agcataatta gaatttatta ctgctccgtc aacatctttt
     3961 aatactgttg gtaattgctc tgcattcatt tcttttatta ctatattttt aggattttta
     4021 gttatatctt ttttagttat aagctctcca tcttttactt ctatcaattt attttttgct
     4081 aataatttaa gtgctctagc tccattagtt gcatcatttg gcacagctat aacagctcca
     4141 tcttttaatt catctaatga cttaactttt tctgaataga atcccattgg ctctatatga
     4201 actttagatg tataagtaag tttatatcct ttctccttca cagtttcctc taaaaatgga
     4261 atatgttgaa agaagttagc atccaaagac ccttcatcta aagctgtatt tggaagtaca
     4321 taatcatcaa atatttttac ttctaaatca tatccttttt cttttaacaa aggttttgca
     4381 acttccaata tctttgcatg tggatttgat gaagctccta ctagtatttt tttatcctct
     4441 ttgtttgaac aacctactgc tgatattgct attgctgaca ccaatgctac tgataataat
     4501 ttctttaatt tcatttttac tccccctctt taaaatgttt ataattaatt tattttagtt
     4561 tcttataagc cagatttcct actccctgga ttatctgaac taatattata agtgctatta
     4621 cagtatatac cattactgct gtatcaaatc tttgatatcc ataaataagt gctatattac
     4681 ctaatcctcc agctcctact gcacctgcca tagctgtata accaagtata gatattattg
     4741 ataatgtaat tccagatact attgaaggca tagcttcctt tatcataact ttaaatacta
     4801 tttgtctttt tgtagctcca aaagattttg ctgcttctat caatccttta tcaacttcat
     4861 ttaatgagct ttctataatt ctagcgataa acggtgctgc cccaatagtt attggcacta
     4921 ttgcagcagt ttcccctata cttgttccaa cgattaatct agttattgga atcattgcaa
     4981 ctataagtat cataaatggg aaacttctaa atatatttac tataaatcct aatgcactat
     5041 ttattgtgct atttggtttt aatccatctg gttttgtaac tactaatatg attgctaata
     5101 taaatcctaa tattgttgcc actatagttg gtactataac catatacaaa gtttgtaaca
     5161 atgcatttgg aaatagtgtt gttaaaaagt caattaaact attcatttat catttcctcc
     5221 catcttatgc ccttactagt tagatattgt tttacttgct ctccactttt atttgaaatg
     5281 tttataataa gtgaacctaa aatatcatct ttaaattgtt ctaatttccc aaatattata
     5341 gacacatcta tatttagttc tcttgccata gtcgtaataa tagcttcatt agaaatatct
     5401 ttagggaata gtatctttat atttgttcct tttggcaata ttatactttc ttcacctatt
     5461 aattttctaa gtcctttagt atttcttaag aatatttctt ctgtatctcc tacctctaat
     5521 acttctccat tttccattat tgctactctt ccacaaatct gctttataac ttccatttga
     5581 tgagttacaa ctattattgt tattcccaag tttttattta tatcttcaag caatgaaaga
     5641 attgactttg tagtatttgg gtctaatgct gaagttgctt catcacaaag aagtacttgt
     5701 ggatttagtg ctaaagctct agcaattgca accctttgtt tttgtccgcc acttagattt
     5761 ctgggtttat catttttctt ttcagatatt ccaactactt ctaataactc taaaactctt
     5821 tttttaattt ctgcctttga gtatccaaaa cattctaatg gcaatgctac attatcaaat
     5881 acagtttttc tttcaagcaa tgaaaagtgt tgaaaaatca ttcctaactc ttttcttaaa
     5941 tctctcattt gcttttcatt caatgacttt acttctttat cagaaacaag tacactgcct
     6001 tcttggtatt cttctaatcc gtttatacat ctcagtagtg ttgatttccc tgcaccacta
     6061 tgacctatta ttccaaaaat ttctccagat tctatttcaa tacttacatc tttaagcact
     6121 tgaatctttc cataatactt attaacattt tttatgctaa tcataaaatt tcctcccata
     6181 tattctgctc agtttttatg tttatcctct cttatgtatt acaacaaaat aaaagcccga
     6241 gtataaactc aggcttagaa agctatttat actcatctat caactatatt agttgcagga
     6301 tttagcacca atgtatataa aatatactgg ttgccgggtt tcaaagggcc agtccctcca
     6361 ccactcttga taagacgtta tttaatttaa ttctattatc atttgttgct atgttatata
     6421 tgatatattc attaatagta tttgtcaata aatttattta aattttttat tttttattat
     6481 cgttaattaa ctatttttaa ttagttttct agctcctaac actttatatt ttaatgtcta
     6541 tataactata gaacatcatt ataagaaata aattaaataa catcaatcta tctaaatcat
     6601 ctgtaaagta aattaagaaa attaacattt tatccaaact ctatcctact attaaataaa
     6661 ataatttaaa tacaatgatg attatatagc tctagtactt tagactttat caaatgttta
     6721 agttctatag gttctattac tgtagcattt ttcccaaaag ttaaaaaata ttcagccacc
     6781 cagttaagtg tagattttgg tatatacata tctatgtatc cacctttaga caagattttc
     6841 aaccctcttg ccaacaaaaa ttctgtttca caacgtttta ctccctcatc tgtcaaatta
     6901 attttaatat gataatctgt acctacttcc atattcttaa gataatcatg tatagaagat
     6961 aaaaccttat atttcttact tgataagttt tctatactca aaatcttcac aattctgtct
     7021 acacgaaatt ccttaataga tgctgatttt atacagtaag ctggacaata ccaaagtcca
     7081 ttcatggcat acaaaccaat aggaacaatg gtacgtacac tatttttttg ctttgatgaa
     7141 tattcaattt taattgtaaa ttcattcatt actatattaa acaattcttt caataatggt
     7201 gttgaacagt gcctatctgg tgtccaaaaa acaaatttac gccttatatt ctcaatatta
     7261 tattgaatat catttggtat acaatttaaa aattttttta aaacagaaat agtttcttgc
     7321 tcaaatggca aatcattata atattctagt gactgatatg caaagaatat agattttacc
     7381 tcactttctg aaaatgcaat cggtggaagt atacgctctt taagtacatg atatccacca
     7441 gccgctccaa cttttgaata taatggaaat ccaatactct ccagcacctg taagtctctt
     7501 agtatagtac gttttgatac agaaaattct tctgctaatt ctcctgctgt aaaacttctt
     7561 tttgcattta ttgttatcat cagttcaatt aaacgctcag ctcttgacat atttatactc
     7621 ctaatttaaa tatagtgaca ataattgtca ctattatatg ataagatatt cttacattta
     7681 aagcaataat aatagtaaag aaagaaggga ctgatacttt atggaaaata atactattaa
     7741 ggctgatgat attatcaatt attgtctatc aaacttagat gatgttttat taatgaatag
     7801 ttggggggaa cgagcaattt actacaatcc taatggtatt ttaaagcgag gagtatatgt
     7861 tcttaccatt aaagaaaaag acagtaatca tgataaaggt tcgttggtta gtcgcccaaa
     7921 tgtataccgt gtgaatatag gattaaaaaa agaaactttt attgaaatgt ttggatatat
     7981 tccaaagcgt ccaggtgtag gtcaaatagt tgatatggat tttgatttta caaaattgga
     8041 cacaatcatg ccacatccta tctactcatg gatgggatgg atatgcgtcc taagccctac
     8101 tgaaaagaca tttgagaact ttaaaacttt aataggagaa tcttacaatt ttgcaaaaca
     8161 aaaatttaaa aagagaaaaa acaagtaaaa acgtatgaaa ataatcccta aataaattat
     8221 aataaaaata ttaaggttac aagataaaaa ttaatatctt aataacatgc aaaaaagcga
     8281 agccaaaaaa tgtattattt ataaggcttc gctatatctc aaataattta tttcaaattt
     8341 caaaactatt tattctatta tttcttatta tttcctgtta ttcctaatct atcattcata
     8401 tcttttatca tttttctaaa accactacct atatcatttc caacatcttc agttccatga
     8461 actaatttta taaagtcatt ttgacctttt ttcatatctt catcagatac cttatctaat
     8521 ttttctattc tcttatctat gtcttttact aatctttccc cttcagcgat taggtcatca
     8581 ttcattttct ttaactctgt atcttctgtt ttaccatctt ttttaagaga tgttacaaat
     8641 gattgtattt tatctctact atctttatat gcagccttta catcacttag atatttttca
     8701 tttcctggat attctttatc tttaaactga ttatttatac tttctggagt atcataaata
     8761 gaatatctat ttagacctgc tatataatca ttatagcttg ttgaataata attcatatat
     8821 ccttcattat tatttacaga ttcattagga ttttttcctg tattttcact tggtttttca
     8881 ttatttttag tacatgcagt tatacctata gctaatacta gtgctaatcc tacaaataaa
     8941 gttcttttta atttcatttt tattaccctc ctagatgttt tatactataa aaaattttaa
     9001 taattttctt tcttaattta gtatttctat atttttatat tttatttact aaaattaaac
     9061 aagttataat ttattttttt ggaaatcata atgaataaat aattaaataa aaaaattata
     9121 attttattat tgatattgat tatcattcat agtataatct aattacttga tattaatttt
     9181 catttgaagg aggttatctt ttatgtcttg ttgtaattca caattttgtt gtaaaaataa
     9241 aattgaatca tcatatataa aatggctttt agaagtttta ggtccagtct ttttaggctc
     9301 taaacctgct gaaataataa atatttcgtt tactgatgta aatagagagg aaaaaattaa
     9361 tgatatttat aaatatctat ataagtgtaa gaaaatagat tttatagtta ttgataaaga
     9421 aaaaaaggga ttaaaaatct tatttgtaaa taaaaaagca ttatcagaaa aattaaaatg
     9481 taaaaaaaca gtaaactttt taaagttttt aggatacaaa caaaatacga atgttaatgc
     9541 ttatttagag catttagtag ataaacttaa gagtgatgtt tttcctgatg aaataggtat
     9601 ttttttagga tatcctctta aagatgtgat tggatttatg ggatatagta attatgaagt
     9661 ttctatgatt aaatattgga aagtttatgg tgacacaaaa caatccgagg acacatattc
     9721 gaagttttta cttcaccgta agaaaatgag aaaattacta gactacataa gtgtagataa
     9781 aatagtaagc tgtttttaat tgtgtttttg tataataata aaaatacacc ctcttttacc
     9841 atacagtttc cataataaat aagggtgtat tttccacttt aatattttaa agtattctaa
     9901 ttttgattgt acaacatatc tttaatattt attttctttt gttgcattcc attagacatc
     9961 aaaaattcct gtgtatcttc taattctttt atatccttgt ccgttatttt agaactaaag
    10021 tcataccaag aatacatttc ttttgtttca tctaaagata aaccaacttc tttagataca
    10081 acatccatca cttcatcctt gttttcattc atatatttta aagtttcttc atgaaccttc
    10141 ataaatcttt ttactaattc aggatatttt tcagcaaagt catcacttac agcagtaact
    10201 attattccac ttactaatcc ttctccattt acaatcattt tggcacctga atttatcgtt
    10261 ttcaatgcca ctggacctgc aagcattgct acatcagcac ttttactttc taatgcagaa
    10321 gcagcactag gaatatccat atttacatat tcaacatcat ctatagtata gccttcttta
    10381 gcaagtgcag caatcaacag ttgatgaagt atagtccctt ttggacctac aattttttta
    10441 ccttttaagt cttttataga tttaatatta tcattattag ttataagcat atatcctttt
    10501 ggtgaacggc tataaatatt cgtaatttta agaccaacac catttgatgc agctattatt
    10561 gcagacgttc ctcctaatgc atgtaaaaag tctagctcac ctgctgctaa tgcttgagtt
    10621 tgctctggtc ctgttgtcaa ttcatggaaa ttgactttta tattgtcctt tttaaattcc
    10681 tttccaaata aatcatcctt tttttctaat atactaggaa cattaagtgg tgattttaca
    10741 taagttaaat ttatttcctt aggtaaacta gcctcttttt ttgcaggttc atctttctta
    10801 tcatttgttg ttgaacatcc tacagtccca accaacacca ttattaaaga tgtcattact
    10861 gctaaaatct ttttgtttct tttaatcaat taaaactccc ccaattccct taatatttct
    10921 tttttcaagt ttatataata gtttttagtt aaatctctat tatattcatc ttcgacacta
    10981 aattgtttaa tccttttatt tttactaaaa acaattattt tctttgctat ttccatagct
    11041 tcttcaatat tgtgagttac aaatactaca ccttttttag tatttttatg gatatttaca
    11101 acttcttttt gcatttttct tcttgtgaag taatctagtg ctgcaaatgg ttcgtccatc
    11161 agcaaaatat cgggattgaa ggaaagtgct cttgctatac ttactctgtg agccattcct
    11221 cctgataatt catttggata tgagttttta aacttttcaa gcttcatcat ttttaaatac
    11281 ttgtctaaat caactttatt tctattatct ttttcagtat gtaaaagtat attttcactt
    11341 acattcagcc atggcataag tctactttct tgaaatacca ttcctacttt tggagtacac
    11401 tttttatcat tattaaaaaa atatatgttt ccactagtag cattttcaag ccctgctaaa
    11461 attctaagta gcgttgtttt cccacatcca ctttcaccta atataacagt tatctcttca
    11521 ctagatatat ttaaagatat attatcaaga actaaatgct ctttattatc aactaaatat
    11581 ttcttatata tattctctag caaaaatcca cttctaacca tttaaatcaa cctgcttccc
    11641 tttgctaaat tttgttatta tctttgaaaa tagataatct gaaagtatac ctaaaaatcc
    11701 aataacaaaa attcctacca atacaacatc agttctagac atatcttttc catcagaaat
    11761 caaatatcct agtcctgaag aagctgcaat aagctctgct ccaattatag ccctaaaact
    11821 atacccaagc cctaaactaa gtccaacaac aatatccagc atagaattag ggattattat
    11881 tttataaaaa attctctttt tagataaatt aaaagattta cctacttcta ttaatttatt
    11941 gtcacaattt tttatacctt tcaatgtatt taaaaatatt ggaaaaaagg atgccaaaat
    12001 aataattata attttagatt cttcacctat accaaaccat aaaataagca ttggcaccaa
    12061 agctagtgga ggtgtacttc gcataaattc aaatagtggt ttgaaatatt catacatttt
    12121 tttatttact ccaaataaaa ctccaagtgg tatagccatc aatgaactta ttaaaaaacc
    12181 tattaatatc cttcttacac tggcataaac atttaagaaa atagaaccgt catatatcat
    12241 ttttataaaa gtatctaaga ctatttcagg aggaggcaga atatagctac tccatatacc
    12301 caaaacacat actatcttcc ataaaattaa aattattaaa aaaatataat atcctttaag
    12361 ccttttatga attttctcaa acatataaaa ctccttttaa catatctatc cctgctaccc
    12421 tttttatttt ttaatatctt aaataataat taagctatat cataagattt aatctatgcc
    12481 aagtcttaat ccacctgaac tcattctatc agaaggtgta gaatctgttt ttaagccaga
    12541 caatctttca gatggttgta taatgactat ttgtccaggt tcaacaaatt ccatatgata
    12601 agattctcct gaagtctgac caataaaagt cttccatgat agttttgata ctttaggata
    12661 tggttctctt ccagtccaag ctactaatgc atctgggtca acacaacaag gtccttcaag
    12721 aattagagga ttaccatctg ttattatagc aacatcttga ttttgacctg ttttatttac
    12781 taaatgtgta gtaaaaagac ctttttgaga tattacccct gaaccaatca attttacact
    12841 gtaatttaat tgttcagaaa atgccaataa gttttcactc tcaacataaa tggaatcacc
    12901 tgggtcaatt gatattacat ctacatggta agcattttga gctaggtaaa tttcaccttc
    12961 accttcaacc accattattg gcatttgttc tcctgtcaat gacctctgaa catgacctaa
    13021 taatgctctt cccaatccac cgccattact tggacctaac agcaattttt caaaattaaa
    13081 atttccttta aaggctacca tagcaccttt ttttgcaaaa aatttaccac ttccccttgc
    13141 tatacacata agctcgtttg taacatcaaa tttaaacata atattcactt atattgctta
    13201 atttagcaat aatccctcct aatcatatat ttatatataa ttatacagta aaattacagc
    13261 atagtctaac ctacttcaaa tatgaaattt gttatttata caaaaaataa gaacctgata
    13321 ataaagcaat ttaacttttt atcaggctct tatcctattt atctcttagc gctatgacaa
    13381 ctcttactac ttgagcatcc agaacaacca catccacaac tgctaccatt cttttttgaa
    13441 tttttaacca tatacattac agctagtgcc attaatacta ctacaatagc tgctataata
    13501 aatgtagcca tattcatcac tccttattta tatgaattaa tttttcatat aacttaattt
    13561 acccattttt tcttttttca tatttttatt tgaataagtt aaaactacaa agactactgc
    13621 tactgctata aatatagata ttattgcccc agcaatactt cctccagtac ctaaaactaa
    13681 acttccaact tgatttataa gaagagcaat tatatacgct gttaaagttt ggaatcctaa
    13741 tgtaatccat gtccatttcc atgaacccat ttctcttttt atggctccaa tagctgcaaa
    13801 acaaggtgca caaagcatat taaatgccat aaatgcaaac gcactagcta ctgtaaacat
    13861 gcttgcaact gaagcaagaa gtgttgggtc ttgctctgtt gcatctgata taccaaataa
    13921 aactccaaat gttccaacta cattttcctt agctactaga cctgtaactg ttgctactga
    13981 tgattgccaa tttccaaatc caagaggagc aaatattggt gcaactatat ttcctaagct
    14041 agctaatata ctatctccag catctaccat ttgtaatgac caattaaatg attgtaagaa
    14101 ccagattact ccacaagcaa caaatataat tgttcctgct ttgattataa atgcttttcc
    14161 tctatcccac atatgtatta aaacaccttt tgcacttgga atatgatatt gaggtaattc
    14221 cataacaaat ggagatggtt ctcctgcaaa taaactagtt ttctttaata taattccaca
    14281 aatgattatc attgcaatac ctaagaaata cattgatgga gctacccatg aagcacctcc
    14341 aaataatgcc cctgcaaata atgctattat tggaatctta gctccacatg gaatgaatgt
    14401 agttaacata atagtcattt ttctatctct atcattctct atagtacgag ttgacataac
    14461 tcctggaact ccacaacctg aacttataag cattggtata aatgattttc ctgaaagacc
    14521 aaacttacgg aaaattctat ccattataaa agctacacgt gacatgtacc cacaatcttc
    14581 taatattgat aataataaga ataaaagcat gatttgtggt acaaatccta gtactgctcc
    14641 aactccacca attagaccat caactacaag tccttgtaac cactctgcta catttaaaga
    14701 agctagaaaa ttacttacat tgccttgaat aatttctcca aacagtacat catttgtcca
    14761 atcagttgct atagtaccca atgaacttac tgctatatag taaactcccc acattataag
    14821 tgcaaaaata ggtaatgcta gtatacgatt tgttacaatc ttatctatct tatctgatac
    14881 agtttcctta ccttttcgat tcttctttat tatacttgaa attattgaac taataaattc
    14941 ataacgattg gctgttatta tactttcact atcatcatcc aattcatctt cacaatttct
    15001 agttatttct tcaatactat tcaatattgt tttagaaata tcaagttttt gtataacatt
    15061 ttcatctctc tcaaatagct ttatagctaa ccagcgtgtt tctacatcta tatatggagt
    15121 cttttcttct attatttttt caattttttc tattgcatcc ttactttcat caacaaaggg
    15181 taatttgaaa tttaatttat tgttactact tgcaagttca actgcctttt cagcagcctc
    15241 cataattcct tgacctttaa ctgctgttac ttctaccact gggcaaccta taacttcaga
    15301 tagttcttta atatttatct tatcaccatt cttatttaca atatccatca tattaagagc
    15361 tataacggtc ggtataccta gctctaaaac ttgagttgta agatataaat ttctctctat
    15421 atttgatgca tctacaatat ttattactgc atccggctta tcatctagca taaaattacg
    15481 tgtaactact tcttctaatg tatatggaga taaagaataa atacctggta aatctactat
    15541 ttccacatct ttatttcctt ttaattttcc accttttttt tcaactgtta ctcctggcca
    15601 gttaccaaca tattgtgatg agcctgtaag cccattgaac attgttgtct taccacagtt
    15661 aggatttcct atgagaccta tttttattga catttccatt ccctccatta ttatttaaat
    15721 acatcactat tcatgatata gaaaatcatt ctcattatat aattacatta actctactct
    15781 aattaatctt tattattcca caataatttt ttctgcatct gacttacgaa ttgatagttc
    15841 ataatctctg actgttacct caattgggtc accaagagga gctacttttc ttataaatat
    15901 attaacgcct cttgtaatcc ccatatccat tatacgtctt cgtgtagctc cttctccttc
    15961 taactttttg acttttacag tctctccaca cttaatatcc ttcagtctgt tcatgtcatt
    16021 acctccctat attattattc tacttgccat ttttttatct aatgcaactc tagtatcttt
    16081 tacgtctatt attaagttac catctatctt agaaactatt ttaacatttg tacctactac
    16141 aaaacctata ctatttaaaa aagtcctcgt cttatcactt cctgaaattt tctttataac
    16201 tacttcttca cctgtatttg atagtatcaa tggcatcata taatatccct cctcttaaac
    16261 taatttaaat ctacaagcaa tcgttatctt atatataaat tacaatacct taaatcttaa
    16321 tctattaaaa taatatccta ttttttcctt ctttttacaa ttcctaattt tgatttaagt
    16381 ctatcacaat agtatatttt ttgcaagtga ttattgtatt cttttgattt tttttctcat
    16441 ttgatttatt ttattaataa aatcgtaaaa attaatacaa aaataaagat gctctaaaat
    16501 agtatactca actaatatta aagcaccttt attatatata tcattactta cacattgctt
    16561 tattcttttt taaaagcaat tttttcaaac tcttttacag gcattggctt agaaaacaaa
    16621 tatccttgta ccatgtcaca cccaatttgc tttagaaatt ctacttgcga aatagtctct
    16681 acaccttctg atattacttt cattttcaat tcctttgcca ttttaatgac attagatata
    16741 acaatttttt cattattact ctcatctttt tctgtaaaaa atgctctatc tagttttaat
    16801 atgtcaacag gcaattcttt taaaagatta agagaggaat atcccgtacc aaaatcatcc
    16861 atagagatta caaatccctt ttctttcaaa ttgttcattg tatcaagtaa cccttctata
    16921 ttatctaata ctgctgtttc tgttagctct aattctataa gtcttgtcgg aattttgtat
    16981 ttatctacaa ttaatttaaa cttctctata aaattactat ttacaaaatg cattcgagac
    17041 aagtttacag atataggtac tactttttgt ccttcatcca tccattctct aatcttttta
    17101 cacacttcct caaatacata gaaatcaata tttacaataa aaccattcct ctcaaataca
    17161 ggaataaatt ctattggtgg tattagacct ttttgtggat tatcccatcg aacaagagcc
    17221 tctgccccta taatttgata atctgaaaga ctatatttag gttgaaaata aacaataaat
    17281 tctccattat taagagcatc taccattgaa ttttcaattt ctttctcttt taatattttt
    17341 ttatgcattt ccttatcgta aaaagcaaat gaatttttat gaccaccttt tatagttttt
    17401 cttgcagtat tagctctatc cataatagta tttatgtcat tatcactttt acttatttta
    17461 tatataccac aatctaaaat caatttataa tagtttattt taggattact aagtatttga
    17521 accttattgt aaatattgtt taaccttgtt ttaatatcat catctgtctt atacttaata
    17581 agaagaacaa aatggtcagc agaaactctt gcaaaaattt catcttcctt taattcactt
    17641 gcaatagtat ttgatatatg tatcaaagta tcatttccca tatcatatcc aaacatatca
    17701 tttatatatt taaatttgtc aacatctata taaaacaatg catattcttc tggattattt
    17761 ttagcaaaca gtttattcga atttatttta aatttatcta tactatctgc tcctgtcaat
    17821 gggtctgtat atgctatctt ctttagataa tcctctttct tctttttatt ataaattata
    17881 tatttaatag caattaagaa taaaatacat acaaaagaaa taatttgtat tgaatattct
    17941 ttaaaaatca caccaaatgg tactttataa ggtttgtcaa tagtattaga atatatacaa
    18001 tcacttaatt cttcttcagt aattttgtct attgatttat ttaaaataga tactaatata
    18061 gggtcagttt tatttgatac ccctatacgc atcccatagc ttgtatctaa aacagatata
    18121 acagataaat tctcactctt tccttctctt aaaagttcat caaactgata agaactaagt
    18181 atcattaagt ttgctttacc ttcatttata gcgtttagac aactttctat actcttataa
    18241 gatgttgtct tagcattttc aaaacttttt tctatataag aatcgataga cttataacta
    18301 ctcgttaaag caatatttaa atcattattg agattggtat ccagttttcc taccataatc
    18361 attggaatat ttatgtatgg atttgtcaat ttcatattaa atcgttttgc cttatcattt
    18421 tcattgattg agccacatag catatttgcc ttaccacttt ttatgtaatc aagactctca
    18481 ttataatttt ttgttttaat atattcaaat tttataccac atttttttga tataattgac
    18541 attaaattgg aacttatacc tttaaacgaa ttgctttcct tatcataata ctctacagga
    18601 gaccactctg aatcatatac catagtaagt tttgggtttg ctttaataaa attcatttct
    18661 tgccgagtaa atgctatgga attattgacc tctcttttga aatatttttt atataattcc
    18721 aactcataat acatatcatt tatttttaat tcttttaaag cataatctaa ttcttttacc
    18781 aaattaggtt tatattttgc agtagcaaaa taaaaaggct ctactgaaaa tttgctaact
    18841 attttcattc ctttagcatt tataatactt ccacagacaa atgcatctgc ctttttttca
    18901 ttgacagact caattaattc atcaatagaa ttacagtaga tatattcaac tgaaaaatta
    18961 ttttcttctc tatacttctc aaatgcaata ttatgaaagt ttcctctaag tacagctact
    19021 ctcattccct taaaggaact tatatcttca taaaacatat tattgttact atcttttgta
    19081 tatagtacac tatattcata tcctgaattt agtttaggaa aattaaatag tttatttctt
    19141 tcatcattct tctgtaaagg tccaagtaag tctatttcac ctttttctag tctgtctaaa
    19201 cattcttccc aagacccttt tacaaattca tattcccatc ttgtatattt tgagatttct
    19261 cttagataat cataaccata tcctgtataa gaatgctctt ttgatgagga tattattccg
    19321 tagtcatcac aatatccaac tcttattttt tttgaatttt cagactcatc agcaaagcat
    19381 agtatattgt taaaacatga tataataagt aaaaatatca aagaaagtat caagaagtgc
    19441 tttttaaatg ctttcatatt taaaaacctc caatttgcca acttacttta acttatttgg
    19501 ttttctagtt gtaactactt ttattgtaat ggtaaatata tcaaattgca atattctcat
    19561 ataatattct tattcaacta taatgaattt attattatct atttaaatac actatctaat
    19621 attagtttct tataagtcat aaaagcacaa ctaacactat aattctagtg attaatttta
    19681 tttcaaaaaa tataataaca ctactaatat tttaaatatc aatagtgtca taaaaacttt
    19741 tcagattaat taaatctttc tatatatttt ataataatct aaatcatgct aaatatttag
    19801 acagaaaact tttttcttta atagtaaata cttttgtttg aatatcagaa taattatttg
    19861 aatatctatc tataaaacca tgatttgcat ctaaaatctc aatttggata ttttgtttcc
    19921 tagaaagttg gtaagaaata gaatcaacat caaatgaatc gtgctttgca aatactaata
    19981 aggtaggaca ttttggaatt acatccatgt aatctcgaat ccgagagcca taacaacaaa
    20041 ttatgccatc acaaaaagaa tcttcactgc atctccatgc aatagttgct cccacactat
    20101 acccagcaac aaatacatat ttatactctg attttaaatc atttataagc ttttcaactt
    20161 ttttataaat atcaaaacca acattattta taaaaaaatc atatgcctct tggacttttg
    20221 aatatggaaa aactcttttt tctcccagaa gttcaggaca aaatacatca tatccatatt
    20281 tataatactt ttgacaaaca tcttctataa agctattaat cccatagatt tcatgtaaaa
    20341 caacaatagc tacataactt ttatgtatat actttatcag attatccacc acctttacaa
    20401 aacagcaaat tttttatact tagtttaata tattcttctt tacatgtcaa tttacttttc
    20461 taaaatcaaa tttatttttg gattaatcat ttatttttaa aagttgtgca ttattactat
    20521 taaattattc tctattatta aaaatataat cgttagaaat aaaaaataaa gtacaaataa
    20581 ttaaaaaata tctcactcat aactttaaaa attgtaagtg agatactcct aaaatctact
    20641 ttatattaaa tttattttcc aaaatatcta tttaataatc ctaagaatgc tttaccatgt
    20701 ctagcttcat ctttacacat ttcatgtact gtatcatgta tagcatctaa tcctaattct
    20761 tttgctttct tagctaattt taattttcca tcagttgcac catactcagc atcaactcta
    20821 actcttaagt tttcttttgt atctgcaact acaacttctc caagaagttc tgcaaattta
    20881 gcagcatgtt cagcttcttc aagagctatt ctcttataag cttctcctac ttctggataa
    20941 ccttctctat cagcttgtct actcattgct aaatacattc ctacttctgt acactcacca
    21001 acaaagttag ctcttaatcc ttcgattatt tcttcatcta caccttgagc tactcctatt
    21061 ctgtgctcat cagcccaatt tttttcgcct ttcatttctt caaatttatc agctccaact
    21121 ttacatactg gacattgtgc tggtgcagca tctccttcat gtatatatcc acatactgta
    21181 caaacaaatt tcttcataat tactttcctc ctaaatttta tatatcaaat taaatttttt
    21241 atttattaag ttagtctata tatacccgca ccatatacct ttaaacaatt tttttaaaag
    21301 ttttttctaa atacttattt tccctgtgtc aataatatgt ttctataata atgtcccttt
    21361 aattttatca attctttatg atttcctgat tccacaattc taccattttc taaaactatt
    21421 atattatcac aatttgatat ggtacttaat ctatgagcta taattatact tgtctttcca
    21481 taagttaaat tatttattgc ttcttgaatt ttagcttctg ttgctatatc tatgttactt
    21541 gtagcctcat ccaaaataag tattggagca ttttttaata ctgctctagc aatacttatt
    21601 aattgttttt gaccttcact taaatcttca cccctatccg ataatacagt actatatcct
    21661 tcacgcattt taactataaa tttatgtgca tttgctattt tactagcata tataacttct
    21721 tcatcagtag cctccagatt tccatacctt atattttcca taactgtatc attgaatata
    21781 taaccttctt gtggaactac tccaaaacaa gttctcaaat agtttctgtt aatagagtat
    21841 atatccttac catctaaaaa tatacttcct tcatctatat gataaaaatt tgtaattaga
    21901 tttataatag ttgtttttcc acttcccgtc tctcctatga tggcatttga tgtgccagat
    21961 ttgattttta ggtttatatt atctaaaatt ttcttttttg aatcataaga aaaacttaca
    22021 tctttaagct caatttctcc ctttacaaaa tgagagttta aattaccatc tttattatcg
    22081 ttaatctcac ttttaaacat gttatgcact tcttgctctt cttgttcatc tataatttca
    22141 aaaactcttt cacatccagc tatggctgaa agtaatgtat taaatagatt tgctaattca
    22201 tttaaaggtc ttgtgaattg tcttacataa cttaaaaaac tagctataac ccctatggtt
    22261 ataatatctt tagatgcaag aatgcctcct ataatacata tagttgcaaa tcctatatta
    22321 ctaatcacat tcataatagg cattaaaaga gaagtgtaaa tttgcgattt aagggaaata
    22381 tttagaagtc tatcattgac ttctttaaaa tcatttacag attttctttc ataattaaaa
    22441 gattttacta catttatgtt tgatatcatt tcttcaatat gtgcatttag ttttcccaac
    22501 tcaacttgtt gttgcttaaa aaaaactcta gttctctttg caataaaatt actcacaaac
    22561 attactatcg gtattaatat tacactgcaa aaggttaaaa ttgggcttaa tacaaacatc
    22621 ataataaaag tcatcaatat gtttaagatt cctgtaatta actgtactaa tgaatttgat
    22681 attcctgtac tgatattatc gacatcattt gtcactctac tcataatatc tccagtagaa
    22741 cttttatcaa aaaaggttat tggtagattt ttaaatttta aaaataaatt acttctcatt
    22801 tctctgacta tactttgaga taatctagct gtacatattc ccttagacag tttaactgaa
    22861 aactcaatta tataaaatga aagtaaaata attattcctt ttaagaaaat ggattttgaa
    22921 taagtatcga tatctattat gtcaactacc tttccaatag tatatggtat tacagttgat
    22981 ataattgaat caattattac tagcaaaaaa ataaatctag taatcttctt atctttttta
    23041 aagtacataa aaattctttt aaacgttttt ttaaaatctt taggcttttc ttttttcttt
    23101 aatctactga ccttctgatt tccagtactt aaattcaata tacttcctcc ttaatttaca
    23161 tcttcactta ctaaattttg caacgcaaaa atttttttat aaattggaga actaaccagt
    23221 aaatcttcat gtgtagcaaa tccaaccacc tcaccctcat ccaagactaa aatcttatca
    23281 cagtcaatta tagaactaat cttttgtgaa atagttatta cagtacattt cttactttcc
    23341 ttttctctta aattacttat atactctttt atattctttc ttataattgt ctctgtatta
    23401 atatccattg cactaaaaca atcatcaaga attaatattt gtggtttttt ggctatagct
    23461 ctagctatag taattctttg tctctgtcct cctgataaat ttttacccct ttggaataaa
    23521 acttcatcat aagtattttc gagttttgtt ataaattgat ttgcatttgc tatttcacat
    23581 gattttacaa catcctcttt gcatatatct ctatttccca taactacatt ctccaatata
    23641 ctttttccaa acacactact attttgaaat actacaccta ttcttgccct taaactttct
    23701 tcagaaatac tgtatatact acgtcctcca atcttaatat cacctttatc aattatatat
    23761 aatttttgaa ttaaatttac taaagaagtc tttcctgaac cagtttgacc aatcacacca
    23821 atattttgac cttcttctat tttaaaggat acgtttttta atattttttc atctgtattt
    23881 ttgttatacg aaaaatctac attttcaaaa attatatcac agtttacaaa ctttaaatct
    23941 ttttctccaa catctaaact ttcttctctc tcaattactt cttcattctc atcaaccaaa
    24001 acctcactta ttctctcata agatgctttt gccctaacaa aaacattaaa aacatgtgaa
    24061 atcattataa gagatgttaa tattctagtc atatagttta cataagcaac tataatccca
    24121 attttaattt ctcctgaata aattaaatgc cttccaatca gtaatattat tattatggaa
    24181 acattaatga ttattgtaat tgtcggtcta aaaaatgaca tcatagttgt agcttttcta
    24241 ctatttttaa aaagttcatc atttaactct tcaaagcttt ggttctcata atcgtatcta
    24301 caaaatagtt taactacttt tattccactt aaaaactgtc ttaattttga atttacttta
    24361 tctgtacttt cttgtacatt tttaaataat ctatacccta tcttcatatt aagcaatatt
    24421 ataataaata taattaaaac tgcaattaga ataattattg acatctttaa atttaatttt
    24481 atagctataa ttaaacttcc tatacacatt attggtgact ttataaaaac tctcatcatt
    24541 ccattaacga attgttgcat ttgattgata tcattagtaa ttcttgttat taatgaagct
    24601 ctaccaaatt tatctatttg ttttaatgag tatgtattta tcttttcaaa tacatcctgt
    24661 cttaaatcat aagaaaaatg ctgagaaact cttgccgaga ttacacttct tattattgca
    24721 aatattgcac ctaaaaaagt tatacctatc attatagctc ctgttgatag tatgtagtct
    24781 aagttcttat ttgaaacccc tatatctatt atttttgcca ttatagttgg ttgagctaaa
    24841 tcacaaaaag cttccaatgt taaaaaaaat actgctgtta gaaataatac tatatatttt
    24901 tttatgtatt ttttaaaata cctcattcaa tcatcccttc atagaatttt ctcattacca
    24961 aaagtatatc ataatggcta atttaattga ttatatcaat tataaatatt tgatttatta
    25021 ttaattttta taccatatat tttataaatt atttgttttt atttcctata tttatatata
    25081 tttatagcat actattatat ataatatata tgtattaaaa ttaagacaaa attttaactt
    25141 tactatttgt gtaaaaagta atataattta aaattgatta taaatctaac ttttttcttt
    25201 atcaaaatat tttaggaggt gagacatttg aaaaaaacta atagtttaaa agaaatttta
    25261 aactggaata atatcaagaa tttaagacct tcaaagggtt taaaaaatat attagaaatt
    25321 tttaaaagtg acctaaaaga tatacgtggc aattatgccg tacttgtaat agttttaggt
    25381 cttgcttttt taccatctct gtatgcttgg ttcaatataa aggcatcttg ggacccttat
    25441 ggaagcactg ctaatatgct tgtagcagta acaaatgaag ataaaggaaa tgaaatagca
    25501 ggacaaaaga ttaatgtagg agaccaatta attgaacaac tcaaagataa caagagttta
    25561 ggttggcagt ttgtagatga aaaaactgct cttgaaggtg ttaaaaaagg tacttattat
    25621 gcatcactta agattccaaa aaattttaca aaagatttga catctttaat tacagatgat
    25681 gttaaaaaag gcgaaattat ctatactgtt aatgaaaaaa taaatgcagt tgcaccaaag
    25741 attacagata aaggtgcttc tacaatacaa ctacaggtta atcagactat agtaaaaaca
    25801 gttagtgaaa tcgttcttgg tatatcaaac ggagttggtg caggaattga acacagtctt
    25861 ccaaaattga caaaagttga aaattccctt attgaccttc aaaagagatt tgaccatata
    25921 aataagactg ttgatttagc ttctgatgct acttcaaaat tatctgatac agttaaagat
    25981 attaaatctg atttacctac aattaaaaag actctgaatg atacaaaact attatcaagt
    26041 gacttaaaaa aattcttaga ggatacaaat gataatttag acgaactttc tcctcttatt
    26101 aagtctgact taaatttaat ggttgacctt tcatcttctg cttcttctct tacattaaat
    26161 ttaattgatg cagtaaatag tggcagtgaa gatgtaccta agttgataga taatttatca
    26221 gaaaaacttt ctaatttgca atctttaaat gatactttgg tggatttttt aactaaatta
    26281 aaccaactta catcaaataa tagattggat gatgtcatag ataatcttga agactctagc
    26341 aataaaatag attcatctat atcaacatta aatgatatta aaaacaaagt tatatctgga
    26401 caacaaccat ctataagtgc tttgaataat gttttaagtc tatcaaatgg tattggaaga
    26461 attaatttaa atattttaaa taattttgac tcaaaaatat caaaacctat aaatagtata
    26521 tttgcaaaca gtataaaagt agcaaatgat ataattactg ttttagataa ggcagaagca
    26581 aaacttccaa aggttgaaga aatacttact acttctctaa aactttcagg aaatgcacag
    26641 gaaagtatat cattgataag agaaagactt cctttagcaa aaggaatgtt ggatgatttg
    26701 attgatactt taagcaaaat tagtaatggt gaagatatga aaaaattagt cagcttgctt
    26761 gaaagtgata tattgacaaa gtctaatttc ttaaaagaac ctgttgaggt gaaaacagag
    26821 aaattatatc caattgaaaa ttatggttct gcaatgacac cattttatag tgttctttct
    26881 acatgggttg gtatattact tatttcagca ctattgtcta catctgtaca tggaaactat
    26941 aaaccttatg aaatttactt tggaagaggc cttacttttt taagtatagc tcttgtccaa
    27001 ggttttataa taggaactgg agatatatta ttgttaggag taacagcaaa acatccaatt
    27061 ttattgatag ttctgcttat gtttactagt atggtattta actttatagt gtattcttta
    27121 gtttctgtat ttggaaatgt aggaaaagca attgcaatag tactattagt cgttcagatt
    27181 ggttcttctg gtggtacatt ccctatacaa gttacaccta cattcttcca aagaataaac
    27241 cctataatgc catttaccta tgcaataggt gctacaaggg aagcagttgg tggtatatat
    27301 gctccaaatc taactaaaga tatactagtt ctattactat ttatgattct gtctatttgt
    27361 ttaaatgtac ttctaaaagg acctataaat agagttataa aaccatttaa tgataaattc
    27421 ggcgatagta atttaactgg tcattaaaaa attaattctg tctttaaata tattttttta
    27481 ataatattct aagttcatat taaaaggtag tagctaaaaa gctactacct ttttccaact
    27541 taaaaactaa tatttactac aaaaactgtc atagaaaaaa acgaaaaaat tcgttctttc
    27601 ctaaatttat cttataaata tttagatata ttcctactta ataagcagag ctatacatag
    27661 tatttaatgt ata
//
LOCUS       DAEMGG010000046        27590 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEMGG010000046 DAEMGG010000000
VERSION     DAEMGG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.046"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            2..319
                     /gene="flgB"
                     /locus_tag="KPK24_001820"
     CDS             2..319
                     /gene="flgB"
                     /locus_tag="KPK24_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG2299494.1"
                     /translation="MYTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            327..731
                     /gene="flgC"
                     /locus_tag="KPK24_001821"
     CDS             327..731
                     /gene="flgC"
                     /locus_tag="KPK24_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG2299495.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            741..1058
                     /gene="fliE"
                     /locus_tag="KPK24_001822"
     CDS             741..1058
                     /gene="fliE"
                     /locus_tag="KPK24_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG2299496.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1083..2633
                     /gene="fliF"
                     /locus_tag="KPK24_001823"
     CDS             1083..2633
                     /gene="fliF"
                     /locus_tag="KPK24_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG2299497.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2646..3716
                     /gene="fliG"
                     /locus_tag="KPK24_001824"
     CDS             2646..3716
                     /gene="fliG"
                     /locus_tag="KPK24_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG2299498.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3709..4440
                     /locus_tag="KPK24_001825"
     CDS             3709..4440
                     /locus_tag="KPK24_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG2299499.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4444..5763
                     /gene="fliI"
                     /locus_tag="KPK24_001826"
     CDS             4444..5763
                     /gene="fliI"
                     /locus_tag="KPK24_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG2299500.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            5794..6240
                     /gene="fliJ"
                     /locus_tag="KPK24_001827"
     CDS             5794..6240
                     /gene="fliJ"
                     /locus_tag="KPK24_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG2299501.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            6269..7474
                     /locus_tag="KPK24_001828"
     CDS             6269..7474
                     /locus_tag="KPK24_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG2299502.1"
                     /translation="MKIQADSYNIIKDIGDRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            7492..8100
                     /locus_tag="KPK24_001829"
     CDS             7492..8100
                     /locus_tag="KPK24_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG2299503.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8127..9110
                     /locus_tag="KPK24_001830"
     CDS             8127..9110
                     /locus_tag="KPK24_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG2299504.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9123..9326
                     /locus_tag="KPK24_001831"
     CDS             9123..9326
                     /locus_tag="KPK24_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG2299505.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9326..10144
                     /locus_tag="KPK24_001832"
     CDS             9326..10144
                     /locus_tag="KPK24_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG2299506.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10134..10829
                     /locus_tag="KPK24_001833"
     CDS             10134..10829
                     /locus_tag="KPK24_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG2299507.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10841..11284
                     /locus_tag="KPK24_001834"
     CDS             10841..11284
                     /locus_tag="KPK24_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG2299508.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIADLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11299..11673
                     /locus_tag="KPK24_001835"
     CDS             11299..11673
                     /locus_tag="KPK24_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBG2299509.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11674..12339
                     /gene="fliP"
                     /locus_tag="KPK24_001836"
     CDS             11674..12339
                     /gene="fliP"
                     /locus_tag="KPK24_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG2299510.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12352..12621
                     /gene="fliQ"
                     /locus_tag="KPK24_001837"
     CDS             12352..12621
                     /gene="fliQ"
                     /locus_tag="KPK24_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG2299511.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            12633..14447
                     /locus_tag="KPK24_001838"
     CDS             12633..14447
                     /locus_tag="KPK24_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG2299512.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            14456..16531
                     /gene="flhA"
                     /locus_tag="KPK24_001839"
     CDS             14456..16531
                     /gene="flhA"
                     /locus_tag="KPK24_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG2299513.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGDLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16506..17522
                     /gene="flhF"
                     /locus_tag="KPK24_001840"
     CDS             16506..17522
                     /gene="flhF"
                     /locus_tag="KPK24_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG2299514.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17519..18397
                     /locus_tag="KPK24_001841"
     CDS             17519..18397
                     /locus_tag="KPK24_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG2299515.1"
                     /translation="MIDQAQILRKIAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18411..19112
                     /locus_tag="KPK24_001842"
     CDS             18411..19112
                     /locus_tag="KPK24_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG2299516.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19128..19550
                     /locus_tag="KPK24_001843"
     CDS             19128..19550
                     /locus_tag="KPK24_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG2299517.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            19535..19720
                     /locus_tag="KPK24_001844"
     CDS             19535..19720
                     /locus_tag="KPK24_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG2299518.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19761..20534
                     /gene="flgG"
                     /locus_tag="KPK24_001845"
     CDS             19761..20534
                     /gene="flgG"
                     /locus_tag="KPK24_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG2299519.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20555..21316
                     /gene="flgG"
                     /locus_tag="KPK24_001846"
     CDS             20555..21316
                     /gene="flgG"
                     /locus_tag="KPK24_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG2299520.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21339..22220
                     /locus_tag="KPK24_001847"
     CDS             21339..22220
                     /locus_tag="KPK24_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG2299521.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22223..22597
                     /locus_tag="KPK24_001848"
     CDS             22223..22597
                     /locus_tag="KPK24_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG2299522.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22629..24032
                     /locus_tag="KPK24_001849"
     CDS             22629..24032
                     /locus_tag="KPK24_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2299523.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLYMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24148..26085
                     /gene="htpG"
                     /locus_tag="KPK24_001850"
     CDS             24148..26085
                     /gene="htpG"
                     /locus_tag="KPK24_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG2299524.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26472..>27590
                     /locus_tag="KPK24_001851"
     CDS             26472..>27590
                     /locus_tag="KPK24_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG2299525.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYH"
BASE COUNT        11400 a         2922 c         5167 g         8101 t
ORIGIN      
        1 tgtgtataca tatgacatta taaaaatggg attagatgca actaatctaa gaagtcagac
       61 aatagcaaat aatatagcaa atataaatac tgaaggatat aaaagacatt atataacttt
      121 tgaagaaaca ctaaatgatg ctatgcctaa aatagaagaa aaaacagaca attctaaagg
      181 tttaagagta gatggtaata atgtagacat agaagaagaa aaagtaaatc aagcaatggc
      241 aacattacaa tataatgctt tgataagttt tgcaaatgga aaaatagcaa tgaccaaatc
      301 aataataagt gggaggtaat tagaagatga gtgtgttcag tggaatgaga atatctgcta
      361 gtggattaac agcagaaagt ttgaaaatgg atatcatatc atccaatgta gctaatataa
      421 atgctacaag aggtccaaat ggagaagcat ataggagaaa agttgtttca tttgaagaaa
      481 actatgataa taaattaggt atgctaggag taaaagttac atctgttgaa gatgataaat
      541 cacctcttaa agaagtatat gacccatctc atccagatgc aaagccaaat ggatatgtag
      601 aatatccaaa tgtaaatata ttggtagaaa tgacagattt aataacagct tcacgagcat
      661 atgattctaa cgtagataca ttgaatgctc agaagtctat gatatcaaaa gctttggaaa
      721 taggaaagta gggataaatt atgccaatta cacaaattaa caatttaatg caaggtgcaa
      781 agttagataa tttgattgga gaagcaaagc cattaactct taataataaa gaagaaaaaa
      841 agaattttga agatgtaata atagatacta tagataaagt taattttcaa caagtaacag
      901 cagatgatat gaaagaaaaa tttataaaag gcgaagatgt ttctatgcat gaagttatgt
      961 taataggtca agaagcacaa ctatctatgc aatatttaat agaagttaga aataagattc
     1021 atgatgcata tcaagaaatg aataaggtgc aaatttagcc ttttgaacag gagtaataat
     1081 caatgataac cagttttaaa aataagatta aggagcaagt tgctaaatct aggacaaaga
     1141 taagttcttt aaaaaaggga actaaaatag cattaggtat aggggttatt gctatatgtt
     1201 tagcgctagt atttacagca atatctgcga gcaaaaataa gtatgcagtc ttattttcag
     1261 aactagatgc tcaagatgct gcagatatat cagcagagct agagtctaaa aagatagata
     1321 ctaaggtaaa aggaagtacg atttatgtac ctagaacaga agttgataag ttaaggctgg
     1381 agttagcgcc aagtcttaca ggtggttcta aaggatttgc actaatggat gatagttcat
     1441 caatagggct tactgatgaa gaatttcaga ttaagaaaca aagaatgata caaggtgaaa
     1501 tagaaaaaac aataaagagt tttccacaag tggaagacac tagagtacat ataacatttg
     1561 gagagtcatc agtatttgag aaggaggctg ttccagggag tgcagcagtt tatttgatgc
     1621 taaaaccagg gacgacttta gaaaaaaacc aaataaagtc tgttatggca cttatatcag
     1681 gaagcgttac aaatatacca gagaaaaatg ttcaggttat tgataacaaa atgaatttat
     1741 tatctgaagg tattttggag gatgaaaatg ttgatgaaaa taatcaacca ataaatagtg
     1801 gcattagtgt tgataaaaat aaggcatctg aacaagcatt gaataaacaa ctagaaaagt
     1861 ctattttaga aatgctagaa ccgatatttg gtaagggaaa ggtgaaagct acagtaaatg
     1921 ctgatttaag ttttgataca gctgaaaaaa ctgagataaa aattgaccct gataaagttg
     1981 caataaagga gacaaggtcg aaaaattcat ctaaatctgc tgcaaataat gttcaaggtg
     2041 tagatgctaa catgaataac caaggaaata acaatggtac taatacagaa gatagtttag
     2101 atgagagttt tgagtatgaa acaggtaaga ttgaaactca cactatagtt gcgcctggag
     2161 aacttaatag attgacagct tctgttgcaa tagatggaaa aatagctaaa ggtgttcaag
     2221 ctgatgtaga ggatattgtt aataatgcta taggtatgga tggagcgagg ggagatactc
     2281 ttagcgttgt ttcaatggta tttgacccag aaggtaagaa agaggctaaa caagagctgg
     2341 aagagattaa aaaagaagga tttaaaaaga acataataaa agcagttatt ggagtagtag
     2401 cagtaatagc agttggagct attggatact taatatataa gaaaagaagt gaacgagaag
     2461 atatggatta tgaagatgaa gattatattt ctaaatctga ttctgttgat aggaaggata
     2521 atcctctatt aggagatgat ccagaactga cattagagga agctgttaaa atgtatgcag
     2581 aagaaaagcc agaccaagta acagagataa taaagacttg gttaaatgag taagggggat
     2641 agaaagtgtc cacggaaatt acaaatgtta atgcgtttga cttaagtgat ataaatgaaa
     2701 gtgataagaa tgagaaggta gaaggtgagg gtttaaaaaa agctgctgta ttattaatga
     2761 cattaggccc aaatgtgtct agtgatataa taaaaagctt gcctgataaa caagttcaaa
     2821 aaataggttt ggaaattgca aatatttctt caatagcttc aaaagaaaga agagaaatct
     2881 taaaagagtt tgtagaaata aatagggcaa aagattatgt tatagaaggt gggcttgaat
     2941 atgcaagaga gttattaagt aatgctttag gaaatgttaa agcaaacaaa ttacttgaag
     3001 gtatatcaat aaatacaagt acaaagcctt ttaaacatat tataaaacta gatgtaaaga
     3061 agatattaga aatacttagg aatgaaagtg cacaaactat agccgtttta cttgcacata
     3121 tggaaccaga aaatggagca gaagttttat catacttaga ttttgattta caaaatgaag
     3181 tagctatgag aataagctca atgggttcaa tatctccaga agtaataaag gtaattgaaa
     3241 gttcattaga agataaatta cattcaataa gtggtgatga ttttaatgat tatggagggg
     3301 tgtctacctt agttcaaata ttgattaatt ctgatagaaa aacagaaaag aatattttgg
     3361 gaactattga agaacaaaac ccggatttag cagctcaaat taaatctcat atatttgtct
     3421 ttgaagatat tgtcaagtta gatgatataa gcatacaaaa agtgttaaaa gaagtgacta
     3481 tgaaagattt agtatatgcc ttaaaaggtg tagatgatga gatttcagat gctatatata
     3541 gaaatcaatc ttctagagca gcagatgcac ttaaagaaga aatggaaatg gttggtgtta
     3601 taaaggttac acaagttgag gcttctcaac aaaagatagc taatatcgta agaaaacttg
     3661 aaagagaagg tgccataact attgtaagga attttggtgg tgactttgtt gaataataag
     3721 attattaaaa gtaaaaaagc attatataaa ggagtgctag atttaaccat aatagataaa
     3781 gttagagtgg gagaatcaaa tgtaaataaa gaaaatgatt gtagatttag ggatgaggta
     3841 ataaaagaag ctatagaatc tgctgagata aaaaagaatg agatattaga agaagcaaaa
     3901 ataaatgcac aaaatataga aaagcaagca tatgaaaaag ggtatagtca aggtcaaaaa
     3961 aatggatatg aggatggata taaagagtca tatgatgaat atacccaaaa agctaaagat
     4021 gaagcagatt ttataaaaaa tcaggcaaat ttaatcttat tacaagctaa tgagaagatg
     4081 actgagtaca taaaagaaaa aaataaagag attgtaagac ttgctattaa tatggccagt
     4141 tctgttttaa aaaagcattt cgaagatgaa gaatctatga gtgatctgtt aaagcaaatt
     4201 attcaagatt atgaaggaag tagttcattt atagtaagat gtaattcttt ctataaagat
     4261 aatatagaga aagagtttga attattcaaa gaggaaacat cactaaaatc aagtgtattt
     4321 gttattgcag atgatagtat agataaaggc aatgctatta tccaaaagga aaatggaaga
     4381 ataatagttg gtattgattg tgctatggaa aaactaaaag aagaattgct tggtgaataa
     4441 agtatgtata aaactgatat atctatagat tttgacaaaa taaataagaa aatagaaaat
     4501 ttagaactca ctatatcaga aggtagagta aagaagataa taggacttac tgtagaagtt
     4561 gaaggaataa aagcttttgt tggggagtta tgcgttatat acaaccaagt taataaacca
     4621 gtgaattgtg aagtagtagg ttttaaagat aaagaagtca tattaatggc gttgggagaa
     4681 ttaacattga tagcacctgg ttgtaaagtt atttctaagg ggataccttt aagtgttatg
     4741 tgtagtgata atttactagg taaagtactt gatggtcttg gaaatcctat agacaactca
     4801 gatgctgttt taggagatag atacaacctg aacaatgaac ctcctgatcc aatgaagaga
     4861 aaaaagatac gaaatataat ggaaacaggt gtaagagcaa tagatgcatt tactacttgt
     4921 ggtgaaggac agagaatcgg catatttgca ggaagtggag taggtaagag tacgactcta
     4981 ggtatgattg ctaggaatgc aaaagcagat gtaaatgtaa ttgctcttat tggagaaaga
     5041 ggaagagaag tactagattt tattgataaa gatttaggtg aagagggaat gaaaaaatct
     5101 gttgttgtgt gtgcaacatc agataaagcc cctctagtta ggttaaaagg tgctcttact
     5161 gctactgcaa tagcagaata ttttagagac caaggcaaaa aagtaatact tatgatggat
     5221 tcagtaacta gatttgctat ggcacaaaga gaagttggtg tagcaatagg agagccacca
     5281 gctcaaaaag gatatacacc atctgtattt gcaatgttgc caaaacttat ggagagaaca
     5341 gggacatcag ataaaggttc tataactgca ttttatacag tattagttga tggagatgac
     5401 tttaatgagc caatagcaga tacaacaaga gggatacttg atggtcatat agttttatct
     5461 agagacttgg caaataagaa tcactatcct tctatagatg tattaaacag tttaagtagg
     5521 cttatgaatg aaattgcttc aaaagaagac ataaaaatag cgtcttttgc aagagacatg
     5581 cttgctgagt atagagaagc agaagatttg ataaatatag gtgcatatgc ttcaggtaca
     5641 aataaaaaaa tagatgaagc aatctattat catgagcata ttattaattt tttaaaacaa
     5701 gggataaatg aaaagagttc ttttaatgaa actataagta gtttgagaag agtttttgaa
     5761 taaacaatta aaaaaatatt tgaataggtt gtgatggaat tggataaaaa ctttaagttt
     5821 agattacaga aagttttaga cttaaaagtg aaagatgaag aagagataaa aatggaattt
     5881 gctaagattc aacaaaaaaa aatagatata gagactaatt tagaaaactt agaaagtaat
     5941 tatagcaaat attctataag taaaaataac gatagtattc aaaatcaaaa aataactatt
     6001 aactatttac tagctttaaa caattctata atggatttat cagaagagtt ggataagtca
     6061 acaaatgaac ttgaaaaagc tagaaagcag cttataagca aacagataga gagaaaatca
     6121 ttagaaaaat taaaagaaaa aaagtatgga cagtattata aagaagaaaa tttaaaagaa
     6181 caaaacacta atgatgagtt tgcaagtatg agttatttaa ggaatagaca agttctataa
     6241 gaatagtagg aagtgaggat attattgtat gaagatacaa gcagattctt ataatattat
     6301 taaagatatt ggggatagaa aaagttcatc taagaacaaa aatataaaaa ataataaatt
     6361 tgaagataat ctatataagg caacagacca taaaaaagtt aaagatagaa gggaagtaag
     6421 taagcaaaat attagctcag ataaaccgga aaatagtgat aatgagtgtc atttagatga
     6481 agataaaagt aatcacttag aagatgaaaa tacatatgaa aaagattgta gtaagaataa
     6541 tgaagctttg cagcaattac tgaatctttt aaaggataat tataaaaaag ataaagatat
     6601 aaacactata ttgaataata tagaaaaact aagtatatca gatgaattaa aacaagaact
     6661 taagcttaat aaaaatatat taaataagat tgcaaatcaa aattcagagc taaataaagc
     6721 taatataaat aactctaatg atatagaaaa tttagacata gttaaattaa gagaactttt
     6781 ggaagataat gctaataaag atattgaagt aattactaaa actacagaaa atgataccaa
     6841 aaatatagat ttgtatagtt ttgatagcaa tagaatggat aatcttaact caaataaaca
     6901 gaaagatgat agctctgata ttttggaaaa actagcaggg gtaaatggaa ataagagtgg
     6961 gaattttgga caagtaataa ataaaaatgt tgatatgaat aaaattaaaa atgataatgt
     7021 caatttaaaa cagatagaat caaatgtaat ggatgactct ataaaagcta ttaagcatat
     7081 gaaaacaaat gatatacaag aattgacaat taaattaaga ccaaaagaat taggagatat
     7141 gaatatacaa ttgttaaaag atggtgaaag tatgagagct gttgtgactg ttttcaataa
     7201 agatgtcttt gactctataa ataaaaacat agcagattta aagcaacatc ttgaacttac
     7261 taatgtaaat ataaaagatg tatcagtgca aatgcacagc gataatacaa atacatctga
     7321 tacctttgat aaagcatttg aacaacagaa tagacaaaat aaccaagaaa acatgagtaa
     7381 gaagaataca aatgataagc ataataatgt agtcatagaa gatgacatta aagaaaatac
     7441 tattgatgat gatagagtag atttactagc ttagtaaggg ggaagtattg catgtacgga
     7501 atagcaacgg ctttggcagg aactcaggga aactataggt caaataactt aagccaactt
     7561 gaaagaaata ttaatgatat tgccaataaa caagttagaa gcaaaggaaa tcagacagta
     7621 acagacaatg ggactccaat tataggacaa ggtgatgatg ctgataaaga cttgtttatg
     7681 aagatacttg tggcacagat gggtaatcaa gacccaatga atccgcaaga cccaacagaa
     7741 tatgtgtcac aattagcaca gtttgcaact ttagaaaaga tgtcagctat gaatgagaag
     7801 ctagatgtac ttttagcctt aagtaattcc actctaataa actctgcttt aagtatggca
     7861 acatcattaa ttggtaaaga agtagaagta tctggggaag agaaagaaga tgaaaacatt
     7921 gtaggagtag taaagtccac atatataaaa gatggggaag tacacttaga attagaagta
     7981 gatggaagtg acaaaactgt aggtgttaaa tatgaaaaat tacttaaaat aggagaggta
     8041 gatgaaaatt caggagaaga aaatgattct tcagaacacg aatctccaga agaagcataa
     8101 tattaaatga gaaaagaggt aaattcatga taaaagcaat gtattcaggt gtaagtggaa
     8161 tgaaagcaaa tcaaacaaaa ttagatgtaa taggtaacaa tgtagccaac gctggaacta
     8221 cttcttttaa gaaaagttca gctagaataa cagattcatt ttatcaaact gtactatatg
     8281 caagtgctcc aacagctgca ttaggaggaa caaacttagg acaagtaggt gtaggtagta
     8341 agatatcaag tataaataaa gatatggtac aaggaaatgt acaaccaact aatagaccat
     8401 ctgatttaat gatagatgga gatggatatc ttccagtagt aagacaagga acagttatgt
     8461 atacgagaga tggttctttt aatttagata caggatttaa agcagctgat ggtgctttga
     8521 agaatattaa aggtggaaga cttgtaaatg gtgatggata tatgttacaa ggtatgattt
     8581 atgatgggga atataaagct gatactggag aatttgttaa aaagggtgat gctaaaggaa
     8641 aagagcctat tgtaatacca cttcaaagta taagtcctaa ggatggaaca gttcaaaatg
     8701 tactagagta taatatatca aaggatggaa cagtagaatt tttattgagt gatggtcaaa
     8761 gaactcaatt tgtctatgat atacctgatg gtgctaaggt aggagataca cctgctggaa
     8821 tagataaatt aaaaggtaca atacaaaaag tgcaaacata tgcattccaa aatccagcag
     8881 gactagatgc agaaggagga aacttgttta aaccatcagc aaactcagga gacccacaaa
     8941 tagcaggagc tacaggcaca ataatacaag gggcaataga agcttccaat gtagatatag
     9001 cagaagaatt tacagaaatg ataatagcaa gtaggtcttt ccaagcaaac agtaaaataa
     9061 tatcgacatc agacgagata cttcaagaaa taataaatct taagagatag taggtacata
     9121 ttatgataag agtaacggat ttaaacggaa gaaaatacat attaaattca gaccttatag
     9181 tgaggattga ttctgtacca gaaagtagga ttctccttac aacaggagaa aaaaaccttg
     9241 taaaagagtc ggtagatgag atagtagata aagtaataaa gtataaaaga aaaataaatg
     9301 taggttatat taggagcgaa aaataatgaa aaaaacagat tcactgacat ttattggact
     9361 aatagtatct acagtattag ttttagttgg tgccgcaaaa ggaagtagtt cagggttaaa
     9421 gaattttttt gatgtttcat caatattaat cacagtgctt ggttcatttg gagctctaat
     9481 gattacattt actatagatg atataaaact tataaaaaat gcattacaat actcatttaa
     9541 gacaatgagt gtatcaaagt tagatttact agaacaattt aaaacacttt ctaaaaaagc
     9601 tagaaaagaa ggtcttttat caatagagag tgatgtaatg ggaatagaag atccatttat
     9661 gaagaaaggg ttagagctgt gtattgatgg attggaaatc aatgagataa ggtccatctt
     9721 agaactagag atagattctg ttgaagatac aaataacaga tactctaaac tatttaaaac
     9781 ttggggaacc tttgcacctg cttttgggat gttgggaaca ctaataggtt taatacaaat
     9841 gttagctgac cttacatctc cagatttaat agcatctggc atgggaaagg cattaattac
     9901 aacgttttat ggttctttac ttgccaatat cgctttgaac ccaattgcat ataatataga
     9961 tgagaagact gaaaaagaaa tttatgtaaa ggaaatgatg ttggaaggta taataagcat
    10021 acaatctggg gagagcagta tagtagttga ggaacgttta gccacatacc tttcaaacaa
    10081 tgagaagtta gaaattatga aatccaataa aaatacagaa agagcaatga gcaatggcgc
    10141 gtaaaaaaaa taagaaaaag gatgagataa atccagatgc atggctggct acttatgcag
    10201 atactataac actgattctt acatttttcg tattattgta tgcaacatca gttgtagata
    10261 tggaaaaatt taaaaacata gcagatgcgt taaaaggtca gttttctggg acaagtatat
    10321 ttagtacggg ttcaagtaca agtgagaaat ctccaataga cagtgtttta gtggataaaa
    10381 atgataaatc aaatttagaa caggatttaa aagataaagt taataattct aacttacaaa
    10441 atagtgtaac agtcaaagat gatagtagag ggattttact agagttagat gattcaattt
    10501 tatttgactc tgggatttct gagttaaaag tagatagtaa agctactctt aataaagtat
    10561 atgacatgat aaagatgatg aaaaataaga tagttataga aggccatact gataatgtac
    10621 ctgtaaaatc aagtacacat gaatccaatt gggatttatc ttctagtaga gcagtaagtg
    10681 ttgtaagata ctttgtagaa gaaaagggca tggaccctag actattttcg gcaacaggat
    10741 gtggagagta tcaacctttg gtttcaaatg aaacagcaaa gggaagggct gagaatagaa
    10801 gagttaatat acttataact attgactagg aggaagaggt ttgttcaaaa ataaaaaaat
    10861 tattataatt attttgatac tgattatttt ctcagcggga gcaggttttt ttatattagg
    10921 agaaggtgga aataaacaag ttgacaagat tgcagatttg tttattccaa caggaaaagg
    10981 taaagatgaa gtaaagaccg aaaatcttga cttagaagag atggtattaa agctagctga
    11041 tgaaggtgta agatatttaa aagtatcaat aactgtatct tatgattctt catataaaga
    11101 tatagaaaaa aatacgcctt taattaggga taatattata aattgtttta tggcaaaaag
    11161 ggcagatgat tttactgctg ataatttaaa gaacataaaa gaagaaataa aaaatacatt
    11221 aaatatacaa ctaggtgaaa atgtaattca aaatatatat ttcacaaata tagtagtgca
    11281 ataaaaggat ggtaagacat gatactttat ttactaaaaa tattaatatg tatcccaata
    11341 attttaatat tgatagtctt atctttaaaa attagccaag gaactttatt aaaaaaatat
    11401 aatgataaat atgtaaaaat actagatatt gtaaatataa gtaaaaacaa tagcataata
    11461 attttaaaaa taggtgaaga aggatgcatt atggcatctt cttcttctag catgagtaaa
    11521 ataaaagatt tgaccaaaga agaaatatta aaaatagaaa acgacattaa agaaagaaat
    11581 tttaaaattc aagattttaa aatggaaaga attaaaaaga aatatgattt cagtagatat
    11641 attggaaaat ttacaaaaaa ggaagataag taaatggaac tattatcaaa attacctttg
    11701 gctgatgtac catatacaag ttcaatgcaa ttgtttttat ttctaactgc attaatgttt
    11761 ttaccatttg taatgttcat gatgacaagc tttgtcagaa tagttataag tttatcattt
    11821 ttgaaatcag cattaggagc acagcaagca attccaagtc aaattttggt aggacttgca
    11881 atagtattga caatatttat aatgagacca gtattaaatg agataaacga aaaggcatta
    11941 caaccatata tgaaagaaga agttactatg gaagaagcta tgaaggaagc agaaggacct
    12001 attaaagagt ttttattaac acaaaccaga cagacagatt tagatttatt tgtagaacag
    12061 gctggtttaa aagaaaagaa attaactcgt gaaaatatac cactatctgt tgtagttcca
    12121 gcatttgcta taagcgaact aaaaactgct tttcaaatag gttttttaat atatatacca
    12181 tttttgataa tagaccttgt tgtggcaagt gttttgatgt ctatgggtat gtttatgttg
    12241 cctccagtca tgatatcatt accatttaag ttattattat ttgtaatggt ggatggatgg
    12301 aacttgatag taaaaacatt gatattggga tttggatagg agaaaaatag tatgagtgaa
    12361 tttatgataa tgagtgttct taaaaatggc atgtttacag gagctatgat tgcagctcca
    12421 gtactcattg tttctcttgt agttgggctt ttaataagtg tgtttcaagc tacaactcaa
    12481 atccaggagc aaactttgac ttttgtacca aaactaatag ctattctttt agtagtagct
    12541 attggtggaa gctggatgtt acatactctt attagtttta cttctgagat attcaatatg
    12601 atatctctta taactaaata agtaggatac atatgatttt tgtatttatt agaatagtag
    12661 cattctttgg aacactaaga atactatttc cagctggtac acctgcttta tttaagtcac
    12721 tctttgcaat cattatatca atattgattt caagtactat gcaaattgaa tatgcaacaa
    12781 atatagacaa cattgtattg tttaccttat atggtgtaaa tgagaccata actgggatta
    12841 tgcttggata tataaccaac ctatgttttt atagtatcag aatggcgggt tctatgatgg
    12901 accaacagat gggattatca atgattaata tgtttgaccc aaattcaatg actcagacaa
    12961 ctttaattga taatcttatg aactggacag cacttatgat attttttagc atggatggtc
    13021 atcatgtttt aattagaggt ataaggtata gttttgagtt aattccaata ggaaaaccat
    13081 ttgtagataa taacatagat tatataataa acatatttgt gcagtgcttt ttaactggat
    13141 tcaaaatagc aatacctatt gtactttgtc tgcttatggc agattttatt ttagggctta
    13201 tatcaagaag tgtacctcaa ttaaatgtaa tgatagttgg tatgccatta aaaatattgg
    13261 ttggtattgc attatttatc atatcaatac ctttaattgt caatcaaata agtcatttat
    13321 taagtcaaat accaaagatg tatgagggaa cttttgcact agctcctatg ttttttatgg
    13381 gttcagcaga taaaacagaa gaagctactc caaagaaaaa gggagaacaa agaaagaaag
    13441 gtaatatagc aaagagtaga gaattacccg ttgcaatgac tctacttgca ttcaccttat
    13501 tagtacctac attattttca tatgtagtag atacacttaa atcttcgctt aattattttt
    13561 tgagtctaga tttttatatg aacataaatt attcaaacct agagaaatta gttatagcag
    13621 gattaatgga tttttttaag atatttttac caatagctat tcccttttta gttttaggca
    13681 taatagcaaa cctatttcag gtgggaatac tgtttactgg tgaaacttta aaaccaaatt
    13741 tatcaaaatt aaatccaatt agtggattca aaaacatgtt ttctatgaga agtttgtcaa
    13801 ccttaataaa ggatacagcg attatatcta ttttggctta cataggatat acattcttcc
    13861 aagataatta tttagatata ttaaagctag gtaacatata cctaccaact ttaatgtaca
    13921 cagttaaaga cttagtatat tcaatattga gtaagatttg tgtagctatg atagctatag
    13981 cagttgcaga ttatgtttat caaagataca gtcataaaaa gcaactgaga atgacaaaac
    14041 aagaagttaa agatgaatat aaaaactcag aaggagaccc agaagtaaaa gctaagataa
    14101 aacaaaaaca aagacaaatt tcgtctcaaa ggacaatgca agcagtacca agtgctactg
    14161 taatagtaac caatccaacc cacttatcta tagcagtgag atatgaaaaa gggaaagacc
    14221 aagctccagt agtggtggca aaaggtgcag attatctagc ttttaaaata agggaaattg
    14281 caaagggaaa tgacatccct ataatagaaa acaaacctat tgctaggttg ttatataagc
    14341 aagtagaaat agaccaagaa attccagagg acatgtatca agcttttgca gagatattag
    14401 tggcagtata taaaatcaaa aatagataca aagtccccaa aaggtaggat aagtaatgaa
    14461 taagtttaat ttaaaggaaa atatggatgt aatagtggca tttggtgttg tagggattgt
    14521 tcttatgatg atagtgccat tacctacatt tatattagat attttactgg caattaacat
    14581 aagtttatct gtgctaattt tactaatgac attatttaca acaaatgtac ttgatttatc
    14641 tatatttcca acagtgttac ttgtgacaac actatttaga ttgggattaa atctttcatc
    14701 aactagattg attttaacac agggatatgc aggtgaagtt atagaggcat ttggaagttt
    14761 tgttgtaggt ggaaattacg tagtaggaat aattatattc ttaattatat taattgttca
    14821 atttgttgtt ataaccaatg gttctggaag agtatctgaa gtaacagcta gatttacgtt
    14881 agatgccatg ccaggtaagc agatgagtat agatgcagat ttaaatgcag gagttataga
    14941 tgacaaaact gctagaaaaa gaagaaaaga gcttcaacaa gaagcagatt tttatggttc
    15001 tatggatgga gcaaataagt ttgtaaaggg agatgccata gcaggtctta tagtaacagc
    15061 aataaacata ataggtggaa ttgtaatagg agctgtaatg ctgaatatga ctcttatggg
    15121 tgcagcacag acatatgtaa gacttactat aggggatggt cttgtaagtc agatacctgc
    15181 tctattaata tctacatcag caggtataat cgtaacaaga gcttctactg atgaaaactt
    15241 tggtgactta gtaggtaaac agttaacggc aataccaaaa gcagtaatga tgactggttg
    15301 tgttctaatc gtacttggta tgatgcctgg attacctaaa ccagcatttt tttcactagg
    15361 tataggagct atagcagcag gttacttttt aggaagagaa aagaaagata ctttagaaga
    15421 cttggagttt gaaaaacctg atatggtaaa cccagataaa aatgaagtag aagatgtaac
    15481 aaatttaata aatgtagaat caatagaagt agaaattgga tatggtttaa tatccttagc
    15541 tgatgaatca gcaggaggag atttacttca gagaatagca tcaataagaa gacaatgtgc
    15601 aatagatatg ggtatagtcg tacaacccat aagaataaga gataacttac agttaaatcc
    15661 aaaccaatac aatataaaaa taaaaggaaa tgtagttggt gggtatgaga taatgcctaa
    15721 tatggtttta tgtatggacc ctatgaatca aggatttagt atatttggaa taaagacaat
    15781 tgaacctact tttggaatag aggcactttg gatagaaagt gacaaggtag aagatgcaga
    15841 gttaaagggt tatacagttg tagattcatc cacaatactt ataactcact tactagatat
    15901 aataaaatca aatgcacacg aattactggg aagacaagaa gttaagacta ttatagatgc
    15961 agcaagagaa aattatagtg ctgtagtaga tgaattgata ccagatttga tgacacttgg
    16021 tgaaattcaa aaagtactta aaaatctgtt aagagaaaaa gtaaatataa aggatagagt
    16081 tacaatactt gaaacgcttg cagaccagtc tagaaataca aaagatatag agcttcttac
    16141 tgaatatgta agaatagcct tggctagaag tatttgtaat aatttagttg atgatgataa
    16201 gacaattgta gtggctacat tgtctccaga aactgaagag ttggtatcta ataatttaca
    16261 gaggtctgta aatggaactt atccagcagt tgacccagaa aatacaaata aaatatttga
    16321 aagtatacaa gaagtaatga gcaatgtata tttcaatcat aatataccag taatagtagt
    16381 atcaccaaag ataagagctc catttagaaa attagttgaa attgtatatc ctaatttgac
    16441 tgtattatca ttaaatgaaa tacctaacga tattaaaatc aaagcagaag gtgtggtgag
    16501 catatatgat gacgaaaaag tatacagcta acacaattca ggatgctatg aatctagcca
    16561 aaatggaatt gggtgacaat attacattaa tagataaaaa agaggtaaga aagtctggaa
    16621 tacaaggtat cttttcaaaa aaagatattg aactaactat tggatgggaa aagagagaaa
    16681 atgtagaaca aaaagattta aaacgagaga tagaacaatt aaagtcaatt attaacaata
    16741 tggggtttga taataagaat gataatgata tagataaaat atgtaaaaat ctactaagtt
    16801 tagaattgaa tgaagaaatt gtagaatcta taagaattga tttgcaagaa atgaagttca
    16861 atggaatcga tacaagtaaa aatttagtag aaattttaaa gaaaaagatt aaaatagaaa
    16921 atcaagtaat aaatggtaaa attgctctag tcggaccacc aggagtaggt aaaacgacta
    16981 caatagcaaa gttagccgca aaattagttt ttgaagaaaa taaaaaagta ggagtaataa
    17041 ctatagatac atacaggatt ggtgctgtag aacagttgaa gatatatact gatataatga
    17101 atattccctt taaaggggta ataagtcctg atgaaatgga attggcactt gacgagatga
    17161 aagattgtga tgttgtatta atagatacaa caggtagagg ctacaaaaac tctatgcaaa
    17221 tactagaaat caaaaatctt atagataaag ctgaaactga taatatacat ttagttttga
    17281 attgtacaac aagagaaagt gatacaaaag caattataga ttcatataga aatgtgaatt
    17341 ttaaaagctt aataattaca aagctagatg aaacaataac atatggctca atatttaaca
    17401 taatgaatta tgcgcaaaaa ccaatatcat atattacaac aggacaaaat gttcctgatg
    17461 atattataaa acctaatgaa gataagatta taaggttact attaggagtt gaaagtatat
    17521 gatagaccaa gctcaaattt tgagaaaaat agcaatggaa aaaagaggac ttgatgagtt
    17581 tatagtagag aatgagaatg tacctaagat aattacaata gcttctggta aaggcggtgt
    17641 tggaaagagc aatctcgcta caaatttgtc tatatgcttg accaaactta ataaaaaagt
    17701 tcttatatta gatgcagaca taggaatgtc taatatagat ataataatgg gggtaaatgt
    17761 aaaagggact ataattgatg taattaatgg cgaaaaaaat atagaagata taatttcaca
    17821 gactaagtat ggtgtaaata taatatctgg aggttctgcc cttaatcata tagaagactt
    17881 tacagaagct caaagaaata agtttataca tagcatagaa caaattcacg atgtggattt
    17941 tataataatt gatacaggag ctggtatgag caaaagccta ttgtcattta tatactgtag
    18001 tactgaattt ttcttaataa caactccaga accgacatca ttgactgacg catatagttt
    18061 attaaaagct ataagtaatt ttggcataaa gagaagtgca aatataataa tcaatagggt
    18121 aattgattta gaagaagcaa aatctacata caaaagaata aaaatggttg tggacaaatt
    18181 tctaaacctt aatctagaat tgtatggata tataatagat gacaaaaaag taagtttatg
    18241 tgtaaaaaaa cagataccat tcattttaga atatcctaca agtattgcat caaaatgtat
    18301 tatagccata gcaaagagaa tggtaagtca gtcaagagaa gatattgttg ataataaaag
    18361 ttttctaaga aagatgttta gtatatttaa aagctaagga ggcgtagtta atgaatagag
    18421 aagaattaat aaaagagaat atgcctcttg taaagagtat agcaagaaaa ttttttatac
    18481 cagggaaagg ttttgaatat caagacttag taaatagtgg agtcattgga ttgattgatg
    18541 caataaataa atttgatgct gaaaaagggg caaagttttc tagttactct tatataaaaa
    18601 taaaatctgc tatattagat gaaattagaa atcaaagtcc aatttctaaa cataatctaa
    18661 ctaaagtaaa taagtataat agagtggtag aaaagttgca atctaaatta ttaagagagc
    18721 caacttcata tgaaattgca aaagaattaa aggtttcaga aaaagaactt catgatatcg
    18781 aaagtaatat agatatgtta aatattgtat cattaaacta tgtaatcttt gaagatacaa
    18841 atgaaactgt acaagatgta ataagtgata gagaagagga agctccagaa aatataatag
    18901 aagaagaaga aaagttagag attctatcca aagcaattag taatttaaat gaaagagaaa
    18961 aattaatact ttcactgtac tattatgaag atttaaatct aaaggagata gggaaagtgc
    19021 taggtgtttc agaatcaaga gtttcacaac tccacagaaa atcaataaga aacttaagaa
    19081 acaagataaa agaacttaaa tattctatat agatggtgat tgataagatg atacaaatta
    19141 tattaatagt agtgtctatt ttaataatag taactatagt tataaattta cataaggaat
    19201 caaaaaataa agataacaga tactttgata aaatatttga gcaagaatat gaagcaaatg
    19261 atagattaat aattgcagaa aaaagaagat tctttttaga caaagtactt gcagaaaaaa
    19321 gaattaatag tcatataaaa gtttatgaaa attataacct aataaaggct aatgaagaaa
    19381 gaaaggaaaa catatttata gaatcaaaaa aggaagtaag acaagaaaat gagttatctc
    19441 ttaaagtgaa acatcttata agttcaggtt atgatgatgt ggaaatatgt aagatgctag
    19501 atattggaaa gggggaactg ttattaataa ggagttgtta caaaatataa ggatattatt
    19561 atgtgataaa aaagtattct taggaatagg tattggtata ttaatatcaa ctttatgtat
    19621 aatgcttttt agtaataatg atatgagtaa ggcagagata gaaaaaaagg ccagagcgtt
    19681 aggcatgaaa tatacaagtg agatgaaagt tttagaatag atgatattgt ttagacattt
    19741 acaaaaaagg aggtcataat gtgataagag gattatatac agcagtttct gctatgataa
    19801 ctaatcaaca aaagcaaaat gttgttgtca ataacctagc taatatggat acaaatggat
    19861 ataaaagtaa acaacttttg actaaaagtt ttgatgaatt aaagttggag ggttacaata
    19921 actacgcaaa tggaactaaa caaaagcaga ttataggcga tataagtcca ggtatttcta
    19981 tggatgaaac tatcacaaat tttaatcaag gacctataaa aactactgat aataaaatgg
    20041 atgtagctat tcaaggtaag ggattttttc tagttagtga tgcagctgga aatcaatttt
    20101 acactagaaa tgggaacttc agaacagaca atcaaggtga ccttataaca agtgaagggt
    20161 attatgtact tgggacgaat actgctactg gtgctgtagg tcatataaat gtaggaaatc
    20221 aaaaatttga agtggcaaaa gataatacaa tatcactaga cggaaatgct atgtataaat
    20281 ttaatattgt agattttaat aactacaata acttaaaaaa agaaggagat aacttatatt
    20341 ctggaggagg agctatgaaa gtaaataatg cacttacaat acaaagtgcg atagagtcct
    20401 ctaatgtaga tatggtatct gaaattaata atatgatgac aatatcaaga gaatatgagg
    20461 caaatcaaaa gataattcag gctatggatt ctaaattagc aaaaatagca agtgagattg
    20521 gttcagtaag ataggtaata ggaggtgaat ttttatgtac acaatgtttt atactagtaa
    20581 aacagcttta tcagcaaatc aaagtaaact agatataata tctaacaata tagcaaattc
    20641 tgattcaaca ggatataaaa aaataaatat ggaatattct gatttagtgg atgaagtact
    20701 aaatagacca tcatatccta caaatggcaa tgatatatcg actggaacag gagctaaggc
    20761 aacagctccc atgagaaatt atgaacaagg tgcattggct cctacagata gtaagagtaa
    20821 tttggcaata gatggagaag gattctttag agtgataaga aaagatggta catatgcata
    20881 tacaagaaat ggtggattta atgtagatgc tttaggtaag attgtagatg ataatggaaa
    20941 tatattagat gtccaatttg atgcaggata taactataat aatacaaata tagatagtac
    21001 aaacctgaca gtttcaagaa atggagagct attcgtagat aataaaaaaa taggaaaaat
    21061 caacttatat caacctatag gaactcaaaa ttttatatct gaaggagata gtttattcgt
    21121 cgcaactgat gcagcacaga ttaaacaagt tgagaaagtc gatatagttc aaggctatag
    21181 agagaggtca aatgtttctt tacaagaaga atttgttgac ttaatagcca ctcaaagagc
    21241 atttcaaatg aactcaaagg gaataaagac agcagatgaa atgtggcaaa tagcgaataa
    21301 tttaagagct aaataattat gtaaggtgag gaatattcat gaatgatatg aaagagtacg
    21361 attttggcaa acctcagagt tattcaagag aaaatttaac ttgtttaaat tttttattag
    21421 ctgaattttg taaaaaatat aaaaactata taatttatga acttaagtgt aattctagta
    21481 tggttgttga tagtatagac caagtaaatt atcagacttt tttagataga gtaacttctt
    21541 catgcgtagt agtacaaaat gcaatggaac ctgttatgaa aaacttttct tttagaatag
    21601 atagaagtgt agctgatatg tggattgata taacttctgg aggaactgga gttgtaaagg
    21661 ataatgatag agaacttaca gaacttgata aaaaagtgct tttacacctt atggatgatt
    21721 tagtaagaaa tatgcactta tttgagggtt ttgaaaatat acaagtattg gatacacata
    21781 cacatattaa tttgccacaa ttatgttctc caactgcacc agtatgcgtt gtagatttaa
    21841 aagtgctaaa tgataatgag tatattggaa gtgtttctct ttgctttcca tacaatggtt
    21901 tagaggctct gttagaaagt atatctgtta tagaattttt tgataatgat gtaggagatg
    21961 attcagagga atttacccaa aaaatctata atagtgtatc taatatagaa ttatcagtta
    22021 ttgcagagat aggaaaagta tcaattaatg ttgaggattt actcaaacta gaagttggag
    22081 atgtaatagt aacaaataaa aaaataaatg attatataga tatatttgta gaaaattcaa
    22141 aatcatacac agcaacacca ggatttatat caagtaaaaa aggtgtaaaa ataaatgatg
    22201 ctgttggaaa agaggtgtag ttatggacaa tagtaatctt atttcagatg aagaagcaaa
    22261 gatgttatta aatgataaag ttgaaagtga agttgatgga gatgatgagt tagccttaga
    22321 tgtctctgaa gttaatcgaa ataatataca aaggatactt gaccttaagt tagaattatc
    22381 tgtagttata ggaagaacta agatgactct aagagatatt ttaaatttac ataaaggttc
    22441 tcttattgaa ctggatactt tggcagacca agatgtggaa atactaatag ataataaggt
    22501 tttggcttat ggaaaagtag tggtggtaga tttaaatttt ggtgtaaaga taacaagtat
    22561 agtaagtgaa gaagacatgg taaaatctct tgtttagaga taacatcaat taatacttaa
    22621 ggcatgaggt ggtacttatt ttaaacttaa atgatataaa aaaagatata ctaaaactca
    22681 ataaaaaaga ctcatgttct ctatataatg aatctattaa taatataaat aataattgta
    22741 tatcaactgg attaaaattt ttaaaacaag caagagattt aaacccagat gatgttgata
    22801 tattaaatct cacaggtctt gtaaacttac taaaatgtaa ttttgatgat gctgtagaaa
    22861 gtttctataa gagttcactt tgtgaaaaaa ctgctctgtg tgaaaagtat ataaacatgc
    22921 ttacatctga ggagttttta attttctttg aaaagtacaa tcaacttata aggttaataa
    22981 ataaaggaat gtataaagaa gctatacaag ggtttaaact tataactgac caatactgtg
    23041 atttaataga accttatgag ttgcttgcct tactgtatga caaggaagaa gaatatataa
    23101 aatttgatga atgtttagaa gtattgaaga ttattgataa ggaaaattat ttattaaatc
    23161 aagaaaataa cacattatat atgtataata gtgctaatat acaaactaaa agtaatataa
    23221 aagtagatga ttataagaat gagttcttac ataaaagtag tagtaaccat aaaattaatg
    23281 attataatga tgtaactaaa gaaaagagtt caagcaaaaa ccagaaaagt tcagattcta
    23341 ataagaaaaa aagtattaaa aaaaataagt acctatatat agccataata ttaggaatat
    23401 tattagtagg acaaagtata tatagttctt ataagatgaa taatttaact gataaaatat
    23461 ctaaaaataa tgaaactaaa aatgaagagt caatagattc taacttgact aagaaagaaa
    23521 ataatataga agaaaatgaa gatatgagta aggcttctaa tataaataag agtaataaaa
    23581 acattaataa agaggatgat gtaaaatcta aaaaaaatac tgtagaaaat gaagatgata
    23641 aagattctga cttatttaca gaggatgaat tgatgtctaa ggcgaatact ttaaaatcag
    23701 aaaagaaaac taaatcttct atacaactat ataagaaagt tgcagatata ggtgaaaata
    23761 aagggaatac atctgaagca acttatcaag ttgctattct cagtgaaaag ttaaaagact
    23821 ataaaactgc tgaagaatat tataaaatgt atgtagaaaa ttactcagaa aaagatgctt
    23881 attttgatga atcttattat aatttaggta tgatgtacta taacaatggt gatttaaaaa
    23941 attctaaatt gacattaaag aagttggtta ataaggtacc aaatagtatg tataataatt
    24001 caaaagtaaa agaaatttta aaagaagaat agttaaatta gataagaaaa ataaattatt
    24061 ttattttaaa ataatatgat tataatatat tgacatacca ttttaaaaaa tttaaaatat
    24121 taattataaa atgaggaggg ataaaaaatg gaatttgaaa aagggagtat ttcaattcat
    24181 acagaaaata tttttcctat aattaaaaaa tggttatact ctgataaaga catatttata
    24241 agagagctta taagtaatgg atgtgatgca gtaagtaaac ataaaagact tgtatcacta
    24301 ggagaaatat cagaaaataa gtcatcagat tataaaatta cagtatctgt aaataaagga
    24361 gaaggtacac ttaaatttat tgacaatgga attggtatga ctgaagaaga gataaaaaaa
    24421 tacataaatc aagttgcttt ttcaggtgct gaagatttct ttaataaata taaggataaa
    24481 atggaagagt caaatgatat aataggtcat tttggattag gattttattc tgcatttatg
    24541 gtgtctaaaa aggtacaaat agatacactt tcatatacag aaggagcgac tcctgtaaga
    24601 tggatatctg aaggtggtac agaatacgaa atatctgaat cagatgctag aaatgataga
    24661 ggaactacta taacactatt tattgatgat gatagtaaag aatttttaga tgaatttact
    24721 gtaagaggaa taataaataa atactgttct ttcttaccag tagaaatata tttagaagat
    24781 gttgaaagat tagaaagaga agctaaagaa gcagaagaaa aagctaaaaa acaaaaggaa
    24841 gatggaaaag aagagattgt agatgcaaaa gtaattgagc ctttaaacga cactaatcct
    24901 ctatggttaa aatctccaaa agactgtact gatgaggagt ataaagagtt ttatagaaag
    24961 gtattcaatg tatttgatga acctttattt tggatacatt taaatgtaga ttatcctttc
    25021 aatttaaagg gtatacttta cttccctaaa ttaaaaaatg agtttgaact gactgaagga
    25081 aaagtaaaat tatacaacaa tcaagtgttt gtggctgaca atataaaaga agttatacca
    25141 gaatttttac ttttattaaa aggagttata gactgcccag acttaccatt aaatgtatca
    25201 agaagtttct tacaaaatga cagggatgta agcaaaattt ctaaacatat aattaaaaaa
    25261 gtagctgata agttgaaatc tctttgtaaa aatgaaagag aagaatacaa taagttttgg
    25321 gatgatatac aaatatttat taaatatggt tgcttaaaag atgaaagttt ctatgagaag
    25381 gtaaaagaat gtatattatt caaaactatt gatgatgagt atataacatt acaagattat
    25441 ttagaaaaat gtaaggacaa acatgaaaat aaggtatttt atgtaagtga taaggaacag
    25501 caatctcaat acattaaact atttaaagaa tacgacttaa gtgctgttgt tttaaactca
    25561 tcaatagata ctcattttat ttcatttatg gaatataaag agaatggagt taagtttaat
    25621 agaatagatg cagacctttc tgatgtatta aaagataaaa atgaaaataa agatagtgaa
    25681 gaaaacaaag aagagatagc taagatagaa ggtctgttta aagaagctgt cggagaaaga
    25741 gtgaagaatt attctgtaga aggattgaaa aatgaagaca ctccagctat ggtattagta
    25801 tcagaacaat ctattagaat ggcagaaatg cagtcaagat ttgctggaat ggatttagga
    25861 atgaattttg aggaagaaaa aactcttgta ataaatgaga acagtccaat aatcaaaaaa
    25921 ttggtttctc ttaaagatga tgaggaaaag aaagataaga ttacgcttat ttgtaaccag
    25981 atagcagatt tagctttact ttctaataaa gaattaaagc cagatgagtt agattcattt
    26041 gtacaaagaa gtaataagct tatgagtctt ttgatagaat tataagattg aatttgaata
    26101 cattgaagca aatttataaa aagtattcaa tatcagtgat aagaaagaag tgtctcaaaa
    26161 tgaacttttc agttcatgag gcgctttttt tatgtgaaat taaatttatt ttaatcattt
    26221 caacaatgaa aaatatattt atctctattt tgagacagac cattctttat ttttttatct
    26281 ttggttaatt ttaaatattt aagtaggttc taataaaaaa ctctatttag ttaaatatag
    26341 ttaacttgat atataataag ataatacata taattaagta aaagattgtt ttttagttta
    26401 tgtttaaata cataattaca ctttgttaaa aacataaaat tattactaaa gtagaaatag
    26461 gaggttttag aatgaatttt aattataatt tgccagtaaa tatcttattt gggagaggca
    26521 gaattaatga tgtgggaaaa gaagtatcta aatatggcaa aaaagtattg atagtaacag
    26581 gtaaaaatag tacaaagaaa actggattac ttgataaaac aatagattta ctaaaagact
    26641 ctaaaataga atatgaagtt tttgatagag tagaacaaaa tcctcttata actactatat
    26701 attcaggagt agaaattata aaatcaactg gatgtgattg tgttttaggt cttggtggtg
    26761 gaagcattat ggatgctgcc aagagtatag ctttttctta taaaaaccca ggagatttaa
    26821 atgattatat atttggaatt agacaaggtg aagaagcttt acctataatc ttagttccaa
    26881 caacatcagg tacaggtagt gaaggaaatt gttttgcagt acttacaaat tcagaaacta
    26941 aagataaaaa atctataaag aagaatagta tgtatgcaaa agcatcaata attgacccag
    27001 aacttatggt tacaatgcca aaacatataa ttgcatcagt tggatttgat gcacttgccc
    27061 ataatatgga agcatatctt tctaatggaa gaaaccctct tgcagatgta caagctattt
    27121 atgggattga acttatatca gaaaacctaa taaaggtata taatgatgtt aatgatttag
    27181 aagcatggga aaaaataaca cttgcaagta ctattggagg aatgacgatt ggtacagcgg
    27241 gtacagcctt gccacatgga atggagcatc ctgtaagtgg tcttagagat gtaatgcatg
    27301 gaaaaggact tgcagctttg actcctacta ttatagaaag ttcatgggat agtgatattg
    27361 aaagatttgg taatatttca aaggtattag gaggaacttg tgcagaagat tgttcagatg
    27421 caattagaaa tttccttaaa aaaatagatt tagatataaa actaagtgat ttaggtattg
    27481 aaaaatcaga tatagattgg ttaactgata attgtatgaa aatatcagtt gcaaatatta
    27541 agagacatcc aaagtatttt aataaggaac aaataaagga aatgtaccat
//
LOCUS       DAEMGG010000047        25531 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEMGG010000047 DAEMGG010000000
VERSION     DAEMGG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.047"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            192..1157
                     /locus_tag="KPK24_001852"
     CDS             192..1157
                     /locus_tag="KPK24_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG2299526.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(1355..3466)
                     /locus_tag="KPK24_001853"
     CDS             complement(1355..3466)
                     /locus_tag="KPK24_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG2299527.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(3768..4475)
                     /locus_tag="KPK24_001854"
     CDS             complement(3768..4475)
                     /locus_tag="KPK24_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2299528.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            complement(4674..6587)
                     /locus_tag="KPK24_001855"
     CDS             complement(4674..6587)
                     /locus_tag="KPK24_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG2299529.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKDIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(6671..7762)
                     /locus_tag="KPK24_001856"
     CDS             complement(6671..7762)
                     /locus_tag="KPK24_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2299530.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(7768..9135)
                     /locus_tag="KPK24_001857"
     CDS             complement(7768..9135)
                     /locus_tag="KPK24_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG2299531.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            complement(9089..9841)
                     /locus_tag="KPK24_001858"
     CDS             complement(9089..9841)
                     /locus_tag="KPK24_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2299532.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(9889..10797)
                     /locus_tag="KPK24_001859"
     CDS             complement(9889..10797)
                     /locus_tag="KPK24_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2299533.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(10819..11568)
                     /locus_tag="KPK24_001860"
     CDS             complement(10819..11568)
                     /locus_tag="KPK24_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2299534.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(11597..12772)
                     /locus_tag="KPK24_001861"
     CDS             complement(11597..12772)
                     /locus_tag="KPK24_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG2299535.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(12787..13626)
                     /locus_tag="KPK24_001862"
     CDS             complement(12787..13626)
                     /locus_tag="KPK24_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2299536.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(13629..14786)
                     /locus_tag="KPK24_001863"
     CDS             complement(13629..14786)
                     /locus_tag="KPK24_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG2299537.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRILLFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPVILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(14802..15524)
                     /locus_tag="KPK24_001864"
     CDS             complement(14802..15524)
                     /locus_tag="KPK24_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2299538.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LVCGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(15536..16609)
                     /locus_tag="KPK24_001865"
     CDS             complement(15536..16609)
                     /locus_tag="KPK24_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG2299539.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(16631..18337)
                     /locus_tag="KPK24_001866"
     CDS             complement(16631..18337)
                     /locus_tag="KPK24_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG2299540.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(18383..19939)
                     /gene="murJ"
                     /locus_tag="KPK24_001867"
     CDS             complement(18383..19939)
                     /gene="murJ"
                     /locus_tag="KPK24_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG2299541.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            complement(20054..21109)
                     /locus_tag="KPK24_001868"
     CDS             complement(20054..21109)
                     /locus_tag="KPK24_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG2299542.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(21204..21890)
                     /locus_tag="KPK24_001869"
     CDS             complement(21204..21890)
                     /locus_tag="KPK24_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG2299543.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(22110..24134)
                     /locus_tag="KPK24_001870"
     CDS             complement(22110..24134)
                     /locus_tag="KPK24_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2299544.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRTNNYPAVLVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(24388..25500)
                     /locus_tag="KPK24_001871"
     CDS             complement(24388..25500)
                     /locus_tag="KPK24_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG2299545.1"
                     /translation="MNSRVKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLAYLLLKKLNVGSTATIITFLCIATFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENL
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
BASE COUNT         7978 a         4638 c         2762 g        10153 t
ORIGIN      
        1 ataaattact tttaaaaaat atattatata tacttttaac tatatatata ggtcgataaa
       61 ttttcttata aattatatca ttgtcttttt tttgtaactg aaacaagtat tgaatatatc
      121 tcaatacttt aatataatta agtgtatgct attaaaaaat atacaggaaa aagaagaaag
      181 aaggggtact tttgtcaaaa ttaaagaaat ttgttatact actggctttc ttggtagtaa
      241 tattccctat aagtgtctat ggatattttt attataaatt atctgctatt catgactcga
      301 gcataagtag tgatttattg gataacaatg accataaaaa tgaagatggg ataattaaca
      361 tactacttat gggaacagat gctagaccaa atgaagattc ttcaaggtca gatgctatga
      421 tgatacttac tattgataac aaacacaatg atatcaaact tacatcatta gctcgtgata
      481 gctatgtaga catccctggt catggtaaac aaaaacttac tcatgcttat gcttatggac
      541 aagctgattt attaatacaa acaattgaag agaattttaa tattgatatt caaaattatg
      601 cttgtgttaa tttcgaatca tttatgtata taattgatgc tattggtggc gttgaggtta
      661 ctgttgaaaa aggagaaata cgagaactaa ataaatttat tcctgaaact tataaatgga
      721 ataaaagtga tgataaaggt agcatacaat acattagaaa ctctggaaag cagactctaa
      781 atggatatca ggcactatct tttgcacgta tcagacacaa cgatacagcc tttgctagag
      841 atggcagaca gagacagatt atacaagcta taatcaagaa aacggaaact ttacctgtta
      901 ctaaatatcc aggtttatta gatgcagttc ttccatatgt taaaactaac atgaagccta
      961 atgcaatact ttctttagga gctcaagttt taaaaatggg cgacttaaat attaagcaat
     1021 ttgaatttcc tattgatgat gaaattcata gtacaggtgg tatatatgga aaagctggat
     1081 gggttcttag atttgaccca gatactttag acatattaca cgattttata tttaatgaca
     1141 tagagtttaa acaataaaaa attcaacata aagtttatta aaaagtataa gattaattac
     1201 taaaaaagaa ctatatcgca taattatttt caagttgctg tgtacacttg attacaaaat
     1261 gcatatagtt cttttttatt ttctaattta tcatgataat tttcatttta atctacattt
     1321 ataaaatact ctgtatttta aattaacttt tttattactt aactaagttt aagaaagtgt
     1381 ttaatgtgtt ttgatttata ccatctccag cttcgtatac tagagtagtt ttcttaggct
     1441 ctaaagcagt tttctgagca gatgttacac tatttccaag cataactact ggtgatttta
     1501 atttagctgc taaaggacct gatgttaatg catctaccaa ttgattactt ttacttacaa
     1561 acattgatga atattcagca tttggataga atttttgtat tacttttcca tttgtttctt
     1621 ctctattttc tccagctatt ctatttttag atacatcatt tgtaatgacc ttatccagct
     1681 tatttattac tgaatcagat atacttagat taccacctat aaaataagca tcttttactt
     1741 ttaaatctga tatccaattg taaacttcat cacttacatt atcttttgat actaatacta
     1801 ttggttgctt ttcttctcct gcttttgaag ctatagaaag agaatctgct tcacctgtac
     1861 cacttgttat atatagtttt tctactggat tagtttttac taattctttt gctatcatta
     1921 aagatgtact atatctatct gaaccaccaa ctctatttat tgatacattt gacaatgtat
     1981 cttttatctc actctcaact ttatctccaa tagagttatt tcctccaatt aaaataactt
     2041 tacttggatt aagtcttttt aactcattct tagtggatgt aggtatatta tccttattta
     2101 ccaatagtat aggagcatca tttgatgtag caagtggtgt tgatgttata ccatcaacta
     2161 tagagtaccc atttactaat actactgtat ctgaatttgt agcccaacct tttttactta
     2221 ttgctactgc tgtgtcatat ctttcgtcgc ctcttaattt ctccacttta acattagatg
     2281 gtggagtaac tggttcttca ctactagaat ataaactctt taaatccttt tgtatagaag
     2341 tactatttgc tatatcttta gctgagaaat acatactacc ttttatttca ctatactttc
     2401 tatttaatgt tacttgttgt actatttctt ttgctatatt ttgtccacca taactcgatt
     2461 gaccttgctt atatatacct tgtcctatgt ataagtctac atttgttcct tttacttcat
     2521 tagcccacca tgccactaat ttagaataat ctgctgcttt aagaccaatt ggccagtaaa
     2581 gttgtggaac tacatagtca atcaatcctt gttttatcca tgctcttgta tctgcatatg
     2641 tacctacata actttcatta cctgatgtat ctgaacctgt tgggtcacta ctcttatttc
     2701 tccatatacc tgctgggctt acaccaaata caacattagg ctttatactc tttatagacg
     2761 cttttacatc tctaagtaga gtatttacat tttctcttct ccaatcatcc ttattttgtc
     2821 cttttccata tttttggtat gttgcagtat cattgaaact tactcctgga tagaagtaat
     2881 catcaaaatg aactccatct atatcgtaat tttgaactac ttctgctata ctatccacta
     2941 tgtactttct aacttctggt aaacctgggt cgtagtaata cttatttcca tgtttaacta
     3001 cccagcttgg atttttcttt gctggatggt ttgctggcaa tttatctatg ctctcatctg
     3061 ccatagttat tctatatgga ttaaaccatg catgaaattc cattcctctt ttatgtgctt
     3121 cttctattaa aaatggtaat gggtcatatc cagggtcttt accttgtgta ccggtaagat
     3181 attcagacca tgggtttata tttgatttat ataaagcatc tgattttggt cttacttgaa
     3241 ccactgctgt atttattcct acacttttta atttgtccaa taaatctgtg tattcctttt
     3301 tttgcttggc ttcattattc ttagtcttag gccagtctaa attataaact gtagatatcc
     3361 aagcagctct catttcctta tcatttgatg aatcagcaaa tgaacttaca ctacacatag
     3421 taagtgtcat tatcaatgat aatactaata tacttatttt tttcatttca tcccccttaa
     3481 ttttttaaat ttattttttc tacaatatac ttcttattaa gtatatctaa aattcatatt
     3541 atctgtcaaa tattgaataa tatagttaaa atttatattt gatatttgat tataatatga
     3601 aataatttat tataaaaaaa acagcaaaga aatttatctt ctttgctgtt tttataacgt
     3661 tcatcactat tttaatatta ctaaactctt aaatctctat gtaaaacatt aaatgtaatt
     3721 atatatttaa tgttaatatt caatcctatg taaatcaata tctattatta ttgtaaaaca
     3781 cgtgtagcag ttataagtct ttttgcataa ttagttcctg gagccatgct agtaactttt
     3841 actacatctc ctgtttgtgg tgaatgtatg aatttagaat ctcctacata caatccaacg
     3901 tgatttattg aaccacctga accaaagaaa actaaatctc ctggttttaa ttctgctctt
     3961 gatactttct ttcctactgt tgcttgtgat cttgatactc ttggtaaatt aactcctgct
     4021 ccatttttat agacataaga agtgaaccca gaacaatcaa aagaatttgg tccagttgct
     4081 ccccatttat atggcttacc ttgttgctta aatgctaagt ttaaaacatt ttgtaccttt
     4141 gcatctacat ttccaccttg aatagttaaa taatctccat ttacccatcc tgtaacattg
     4201 tcaacacttt ttattttaat ccatccattt gaatgtaatt ctaaaacttc aactttgtct
     4261 cctgtgttta tctttcctac tactgcatca tttgcactag ctccacttct tacatttaaa
     4321 aaatcacact ggtctactgt tgcactcata ggtgaattag ttggctctgc tgcactagcc
     4381 gttattgctc ctgcttctac tgtgacaact cctgctatta acaccatagc tataaatttt
     4441 ttcaatatat gttttttatt tttaatattc atcataatct caaataccta atcctttcga
     4501 aaatttaaca ttttgttcct tcgtttccaa ttcctaatta acattctatc ttacatcaaa
     4561 ataaattgca accttttttg taatcttttt gtaatctttt tgtaatcttt tgtttctttt
     4621 tagtatatat ttgtttcttt ttgctccccc tggttacaaa ttagaaatat attctacttt
     4681 tctctagttg ttgcaatttc tttgatatta gcatcttctg gtttaatata agttggtact
     4741 aattccctca taataccatc tattttttca acctcttcat tgttagtaat ttctcttaaa
     4801 agttttaagt attttgttat tttttctcta tcaacatcta ttggtctacc aataaaaatc
     4861 tttttatgct ctgtatccaa tagaccttct tctgacataa gtagttcttc atacaacttt
     4921 tctcctggtc taagaccaga aaattcaatt tttatatcaa catctggctc aaatccactg
     4981 aatttaatta agttttttgc taaatccaat attcttacag gttctcccat atcaagcaca
     5041 aatatttctc cacctttagc catagcccca gcttgtataa ctaagcctac tgcttctggt
     5101 attgtcataa aatatctaat aatatctgga tgagtaactg tcactggtcc gccatcttca
     5161 atttgcttct taaaaagtgg aattacacta ccattactac caagcacatt tccaaatcta
     5221 acagcaacaa attcagtctg gctttcagca ttcatagtct gtataatcat ttcagcagct
     5281 cttttagtag ctcccattat gttagtaggg tttacagcct tatctgtaga tataaggaca
     5341 aatttcttag ctctatattt tgaagacaag ccagctatgt ttaaagttcc aaatatatta
     5401 ttctttatag cttcgccagg gctcgattcc ataagaggaa catgtttatg tgctgctgca
     5461 tgaaatacca cttctggttt atatttatta aatatttcat ccattctttt ttcttctctt
     5521 attgatgcta ttactgtctt caaatctaat ttatttttat attttctaag taattcttgt
     5581 tgtattgagt aagcattatt ttcataatta tcaactataa taagctcttt aggattaaat
     5641 cctgcaatct gtctacaaag ctctgaacca attgagcctc ctcctccagt tattaggatt
     5701 acttttccct caatgtaatt acttatctct cttaaatttg tttttatagg ctctctaccc
     5761 aacaagtctt ctatctctac ttctctaatc tgttttatat caacttttcc atctataatt
     5821 tcgtaaattc ctggtattgt ctttatttta cagttagtgt ttttacatat atcaataata
     5881 tctttttttc tacgtttctc tatgtttgct attgaaaaga ttatctcatc tacattgtac
     5941 ttttctacca catcttttat atctgaagtt gttcctacaa ttttcacatt atggattatt
     6001 cttccaattt tatttttatc atcatcaatt atagccactg ctactttttt aagttctgga
     6061 tttcttttta gttcctgtat aaccatttct cctgcatctc ctgcaccaat tataaggata
     6121 tttgaaacct ttcctctaga atatgtcttg ttcatgacta ttcttatagc tctataggct
     6181 attcttgcgc cacttgtaaa cattataata aatattgtat ttaatacata aaacattata
     6241 ggataatcaa accctaataa tttatttatt atataaatcg gaattaaata agctaaagtt
     6301 gccacaaaaa tagatataat ttcttcttct cctgcatatc tccataatgt gttgtagcac
     6361 ttaaacaatc taaatgatac tatatgaagt actatatata tacacactga aattattata
     6421 tcattagtgg taaatgcatt taaattacct gtcaagctta aagacattcg aaacgctact
     6481 agtacgctac aaatatctag aattaatagt gttaaatgtc taaatttata ttttttaaaa
     6541 agcctattaa aaaagctttc tttctctaaa tctatacttt ctcgcataac tagaagccct
     6601 cctttaaaca gcaaaagcta cgcacctaag catagccaac aacatctatc ttttgctgta
     6661 ttttttcatt ttatttttta tatttatcaa atatagaatc ataaattttt tctatatcat
     6721 taaagtttaa attaacctca taattttctc taacaaattg acgccctttc ttacccatat
     6781 taagtctaag ttcatcattt tccattagtt ttattatagc attttttatt tcttctggtt
     6841 gttttggttc tacaattaag ccagtttcat tttcccatac agattctgga tgtcctccta
     6901 catttgtaac tataacaggt acttcacatg cttctgattc tatagctgcc actccaaatc
     6961 cctctcttaa agaaggaaat acagttacat caaaagaatt aaaagtcttg cttacatttt
     7021 ctggagatat tcttcctaaa aactgaacat cattttctat acccaattct ttagctaaat
     7081 ttattaggtt gtccatctga cttccagagc cacctatttt aagaattatt ttcttatctt
     7141 tatattcatc tttaagcatc ttaaatgctt gtattaaata ttctattcca tatttttttt
     7201 ctaaactctt tataattcct attacaaaag cgtcttcttt ttctacttcc ataggcttaa
     7261 acctatccat atctactcca aaaaaggtta catctacatg tttagctgta tatttattag
     7321 cctctttagc catatctcta ctatttgaaa agatatagtc tgcttttcta aagttatgtt
     7381 ttataatttg tttttgtata aaaccattat tagggaaatc ataaatatca gtaccccaaa
     7441 cagaaataat atatggatga tacccaagta gacttcctat aaatccataa ctacttgcat
     7501 actgtgcatg caatatatct ggctttattt catttactag cttctttatc tgctttattg
     7561 aacctaagta tcccgttttt ttagaaatat tttcattttt tagttcttct acattagaac
     7621 caaaattata aatttttatt ccttccatgt gacctccatt taaagagatt acatgaatgt
     7681 catatccttt atttttaaaa aaactacacc attttaaagt atgaggattt gacccatcag
     7741 ctaaataaca aacagtcttc ataatattta ccttcctatc gaataatact caattcctaa
     7801 ctcttccata tctttaattt tataacaatt tcttccatca aggactaaag gatttttcat
     7861 cttttcctta tacttatgta tatcataatt taatacatct ttccattctg tcattataaa
     7921 acatatctct gcatcttcta tcgcatcatc tacagaatca gcataaagta tagagtcacc
     7981 aaatattttc ttagtattat ttacaccaat tgggtcgtat acagtaactc ttgcccctct
     8041 ttcgagcaaa tattttatat ttggtataga tggtgcttct cttatatcat cagtgtctgg
     8101 cttaaaagtc aaacctaaaa cagcaacctt catatcttga aagctctcta ctaacttaga
     8161 tgcctttcta actaatttaa gtttttgact ttcattaact tctattgcag cttttactgt
     8221 ttttaattca taaccattat gttcagataa ccagtgaaga gcttttgtgt cttttggaaa
     8281 gcaagaacca ccatatccta ttcccgcctt taaaaactta cttcctattc tatcatcata
     8341 actcattcct aaagctacat cttctatgtt agctcctgct atttcacaaa gatttgcaat
     8401 atcattcata aatgatattt taagagctaa gaagttattt gatgcgtatt ttatcatctc
     8461 agcactattt ctatttgtaa ctattatagg ttgattaaaa ggcttataga tttcctctaa
     8521 tatttctcta gcccattcac tttctacacc tataacgatt cttgaagcat gtagagtatc
     8581 ttttactgct gttccttgtg ataaaaactc tggattagat gcaagttcta cttttacatc
     8641 attttttagg tttctattta tataatcttc tatctcttca tttgtaccaa ttggaactgt
     8701 agattttatt actactagag tatcttttgt cacagtttca gctatttgct ttgcaactgc
     8761 ctttatataa tctaggttgg ctgaaccatc acttctctca ggtgtaccta cagctattaa
     8821 tattacatct acatcttcat aagctttttt atagtctgta gtgtaatcta ttcttcctct
     8881 gttgtaattt ttaaccataa gttcttctag gccttcttca tatataggtg ctattccaag
     8941 tttcattgat ttaacttttt gttcatctac atcaacacag actacatttt cattgcctac
     9001 ctctgctaga caaacacctg taaccaaacc tacatatccc gtcccagcta tagctatttt
     9061 cattttttta cctccaagta tataaaactt atactctttt tttaatggca ttaaaaacat
     9121 agaaacaaaa tacatataga ctttttggta aacttaaatt ttcaacattt ctataagtat
     9181 tccaagttcg ttttaatgct tttatcttgt tacttgatat agaatttcca actaatctgt
     9241 aattcactaa atcttcttga atcccatatg catatttttc ctttttaagt aactgtaacc
     9301 aggttgcagt atcttgtcct cttcttacca aaggcatgct gaactctcct acaatctcct
     9361 tatcaatgac cactgtagaa catcctatta tagtatttct aagtaatcct tcatagtcaa
     9421 ttttcttagg agctcttgca accttattcg tttttgaccc atcttgtctc atatatctat
     9481 aagatgtaaa agaaaaacct acattttctt ttaacatata ttctatctga atctctaatt
     9541 ttctactatc ccataaatca tcactatcta caaatgcaat aaatcgacct tccgcattct
     9601 taataccaac atttctagat actgcagctc cagaattttt ttcaagcttt atatatttta
     9661 ttctagcatc ttcttttcga aaacttttta taatttcact tgaattgtcc ttagaacaat
     9721 catccactaa taaaagctgc caatttttat aagtttgatt ttggattgac tttatcgtct
     9781 ctgataaaaa ctcttctgaa ttataaacag gagtaattat cgatactaaa ggctcattca
     9841 tatttttact ctccttatat agcaactagc agtactatac caacaagttt acttagatac
     9901 taattttctt tgtccaatat atttgtaata tcctaatggg aataatgcta aatacaacaa
     9961 tttatcagat acttttctta attcaccaaa ataatgacta tcagacaaca atgagtatct
    10021 ggctaaattt atgtaatttt ttatccacat atatggatat ctacttaatg gatatgtatt
    10081 ttgatttatt aaatataaaa actctaatgc tctacctctt aatgcatttt tacttaatat
    10141 attcttagtc aaaccatcat ccaaataatc tcttattatc ataggtttat ttacacatat
    10201 aactttatag tatttagaca tttcattcca tactacactt tctggtaaaa atctcacttc
    10261 ttttctttct ggaaaaggaa attttataag ctcatcagtt acaaatacta tgcttttatc
    10321 tcccttaacg ccatacttaa aatataggtc tgcaaagtga catattttct tatcttcagg
    10381 catttctgtc cctatcaaat ctccacttgg atatacacaa agtccaacaa ctccactaaa
    10441 tccttcttta ttctctaaag aatcccactc atcattagca atttcaagag aatctttaat
    10501 taaaccatca tcactatcta caatgaaaaa tagctctcca tctgcatttt ttacacctag
    10561 atttattgca gtatgttttc ctccattttc tttgtatata tatcttatat ttaatttatt
    10621 ttctgatata aatttttcaa ctaactcttt agtactgtcg ctacttccat catcaacaat
    10681 caaccattca aaatcattga aatcataagt ttgagctttt aaatcattgt acaaattttc
    10741 aagtaaatga gctctgttat acgtcggagt aaatacagta aatttatatt tatacattaa
    10801 ttaaatcttc ctctctgtct aacttttttt gattccgttt attccataat taataaagta
    10861 gtatatagct ttgtataaag gtattttttc tatatttcta tatatattcc aagtccacat
    10921 agcagctttt attttattac cacttaggga attgctagat tttctatata atgctagaac
    10981 ttcatttatt ccatgagcaa tatgcccttt tttcaatatc gataaccata aaacataatc
    11041 ttcatgtctt acaccactca ttcttatttc aaaatccaat ttagacttat caatcacaac
    11101 agtaagacat cctaagatat ttccttttaa taatcttcta taatctacat taggtggaac
    11161 ttttattact ttgtttaatt ttttatcatt ttcatccatc agttcatatg aagtaaatga
    11221 aattacatag tcattttcta acataaaatt tacttgtttt tctaacttac tactattcca
    11281 ttggtcatca ctatctaaaa aagctataaa ttgtcctgtt gccttactta gtgctaaatt
    11341 tctagcatta gagacaccct tattagtctc agtctttata cattttattc tactatcctg
    11401 ttgcatataa gatttgacta tattaggact attatctgtt gagcaatcat caataattaa
    11461 catttcccat tcttgataag tttggtttaa tactgatttt atggttgctt caataaattt
    11521 ttcagaatta tacatgggag taattataga tactaaattc ttcttcatat taccttcctc
    11581 atcatttata aaatatttat ttaattttac ttaatacaca atcatatatc ctcttgcagt
    11641 tcttattatc attatatctg aaaaacttat ttctttcatt tttttggtta tccataacct
    11701 caaaattatt ttcaataata tcaataagtt tattcacggc ttcatcatta ttataagcca
    11761 aagacccaaa catttcactt tttaaatcta tatatgaatc aactttttcc atatattcat
    11821 ccaaatcgaa ttggtaaaac aagataggtc tattcatata caaaaagtct atggctacac
    11881 ttgaataatc agttatattt gcagctgatt ttctgatttc atttgctaga gttacccctt
    11941 tatctaaaaa cacaattcgt tttcctgtta tattttcttt tatatcatct ataaactcat
    12001 gcataagatg atgcatataa atttttacat ttatatcttt ctcatctaat attttattta
    12061 atttgtcatc atttaaaaaa ttcattatat ttttaaaata atctgtatcc gtaaattttc
    12121 ctgaactaaa ttttatccaa tctctccatg taggagtata taatatatct cgagtttgtg
    12181 caacttcatt tgttggtaaa ttgtcatatc ttgcatttcc aattatataa actgagtcat
    12241 ctaacattcc ccaatcattt accataacat ttctttcaaa ttcagaactt gctgtaacta
    12301 tattatagct tttatatagg ttatctataa tatttttgaa ctttttgttc ataggttttc
    12361 tttttttaaa acttatagtc ccatgattta aaaacacttt tatcgttttt ttatgaaaat
    12421 aattaagaac aggtacagca aaattgtatg gtgctatatc tgctgatggt gcatgtgaaa
    12481 atactatagc ttttgaattg taataataca gatagttttc tacacttcct cttattaatt
    12541 ttttgcctgg aattttatca aatactttac tgtctttatt tactacccaa taagtttcta
    12601 catcattatg ttcttttagc atatactcat aaaaaaactt tgaattatca ttgtatatat
    12661 cccctgaatg tccacctata agccagatat ctctaccatt aaacttatgc ttattaaatg
    12721 gatatataaa aaaagccaaa agcatattta ttaaaaattt aaccttgtcc ataaacacca
    12781 tcctcattat aacttgtact gtctaaatcc attaattctt ccttttatag tggcactatg
    12841 ataatctcta ttggaactca atataagacc tcctatattt cttacaagtt ttgataccat
    12901 aacttttgca aacttataat tttttctata gactatttcc tttttgcaaa gcgctccaaa
    12961 gcctcttcca tagttataat ctttttgata cttttcttta gatttagaat gtttttttgc
    13021 aggatggtaa attatatcat ttccaaagta ttttccttta aaacctaaac tcaataagtt
    13081 taaaacataa tctatctctt caccagcacc aaactctcca ccaacaccca atttttcatc
    13141 aaatcttgta tagtctttac cttcaaaatc aataaaaaaa gttatggatg ttatagtatc
    13201 caaaacgttg gaacttgtga tatcacaagt tccatcatac attttcttaa atgcatcaac
    13261 tttatttgaa tccatagttt tgcaactgta tattttataa tctttatttt tattaaagaa
    13321 atttataact ttttctaatg tatcattttc gtagacacaa tcatcatcag ggaaacctat
    13381 aatttgccct acagcattgt ctatgcctac atttctgtta taactcaatc cttttttaga
    13441 gctttttata tactttatat ctaacttatc aatgtattta tcaactattt ctttaacttt
    13501 gctattatca ttttggtcta ctactataag ttcaaagttt ttgtaagttt gattttctaa
    13561 gctgtccata agtaagtata tatcatcata tctatttaat gttggcatta ttaatgatat
    13621 taacattact acctcctact tttcatcttc cacaaatcta tttccctttt atcatttaat
    13681 atcacttctt ttattagtaa tactactgca aaactaaatg aagcttctaa taataaattt
    13741 tctgcaaaag agtatagtaa aaatataaat aaagctacta aatatgcttt tttatctttt
    13801 tctataaagc tttctattcc tttatacatc aaatacataa ataatataca agcaactact
    13861 cctagtacac taattatata tacataagaa ctatctaatg gattagttgc ttttatagca
    13921 ggactatatg aatttccaaa aggttttaga aagttcccct tttcagccaa gtatacattc
    13981 cagaacttag gtctacttgc taataatcta tttaataaag tgcttttatc aaatactgta
    14041 ccaataatta cactaaataa agtaagtatt accggtaatg tctttaggag cattggtagt
    14101 ccttttattt tttttatatc tatccatctt attatttcta caaatattaa tcctgctaat
    14161 atagtgaaaa atccagttct actatatgtg gtttgataaa caaaaaatgc agaaccaaat
    14221 aaaagtattc tatcccattt attataatct ttatatctta agaatatata tgctgcatat
    14281 atagtaacca tatgataaaa tacactattt ggattaccaa gtccaaaatc cattcttgta
    14341 acaagcatat ctattttttc tatatatcta tagtggactt cctgattaaa tattaatgaa
    14401 ggaaataaat taaataataa tatagctacc attaaaaaac atgccgatat tattaaataa
    14461 tttttaacta taaatttatc atctatttgc ttagctgata cagctaataa aacgattaat
    14521 aatatagctc cttccctatt taataaataa agtaagacac aaactccaac tagtaccatt
    14581 tctgttatac tttttctctt atcaaataaa aaaagtagca ttagtattcc tattgaagaa
    14641 taaaataacg ctgatttaat catccctata tccatatttg ggtcaataaa tgatatcata
    14701 tactttctaa gtaatatgat aaacaaaaca aatatataaa gatattttct aatcataata
    14761 tttctatcat catttaaagt cttcatttta ctttctccct tttagcttcg taaagtatta
    14821 cttgcaaata ttttacaaca cttccatatc ttatttacac ctgttaagtg tttgctttct
    14881 aagaaataat ttctcttttt agcccaactt tttccccacc aatgtatccc aaaagtgttt
    14941 tctgttatac attctggtgt aaagtcttct ttaaatccaa aaggataaaa atattctttg
    15001 tcgtaaacac gtattccttc ttcataatta tttggatcaa tcttcttata attcttttcc
    15061 ataagatgag tcattatctt tggtattgta aacaatgaag aagtaagtac ttctttttca
    15121 taatactcca ataagtcttt taaaaaagga tgtccttttt ctacacctat tatagcacca
    15181 tttatatact ctctatcatc ttcataccca cagactagcc tattattttc taaaagagga
    15241 gttatatcct ttaatatttg catatctgta tccatataaa ctccaccttg ttcatagagt
    15301 acttttattc ttgtataatc agatatgaat gcccaaagtt ttcttttata acattcttcc
    15361 aaaaacttat ttccttttat ttctttctct atatcaaagt ttttttcatt ccactctact
    15421 atctcatatt ctggaagctt ttctttccaa gaatttatac atatattttc tatatttcct
    15481 tttggaccac caaaccatac ataatgtatt tttttaggaa tcaaatgaac acctcctaca
    15541 tatattcttg tctatcttct tcttttaact gctctactat ttttttgaca tcttgagctt
    15601 tatcttttct acaaaccata actgcatcat ttgtagatac tactatcaaa tcactaatac
    15661 ctattgttga tattaatttg tcatctgaat atataattga atttttagta tctatagtaa
    15721 tattttcacc tcttttgata ttaccttcat catcagttgg atatattgca cctagagaat
    15781 cccaagaacc aacatcattc catccaaaat caccaggaac tactataaca tcattacttc
    15841 tttccataat accataatct atagagattg attgtatagt tgggtatatt tcttttattt
    15901 tttccatttc ttcttttgtt cctaagtatt tacttatatc ttctaatttt tcatatactt
    15961 ttggcaagta tctcttaaaa tcttctagta ttttagatac cttccatacg aacattccac
    16021 tattccatac atacttttta gagtttacat attctttagc aatttcatag tttggttttt
    16081 ctacaaactc cactacctca tatgcaacat cttctatagt attttctcta ttaaaattta
    16141 tgtatccata tcctgttgat ggaaatgtag gagttatccc aatagttact agtttatcat
    16201 tattttctgc tatatagata gctttttcca aaatagattt aaattctttt tcatctttta
    16261 tataatggtc agctggatat acacacataa caccatcacc atattttttc attatgttaa
    16321 atgctgcaaa gcctatagct gctgctgtat ttctggcaca tggctctggc aatatattac
    16381 tatccaaaca tttatctttt actatatcct ttaaagcttc caattgcttc tcattggtta
    16441 ctataaataa atcatctttt tttgctagag aatctattct gtttatagtt tcgtttatta
    16501 aagcatcttc tccacttaaa tttataagct gtttaggtac ttcttgtcta ctaagtggcc
    16561 agaaacgagt tcctcctcct ccagccatta tgacattgta tactttcatt gtttatctcc
    16621 ccttagcttt ttatatagaa tccactacat ttaatatata agatattaaa ttgttcaact
    16681 taaattctac atcttcttga ttgtctgaat ttgctccaaa gtaaaattta attttaggtt
    16741 ctgtacctga aggtcttata gctatccaag agccatcttc aagtaaaaat ttaagtacat
    16801 ctgacttagg taaatcatca acaccatctt tatagtctaa aattttatct acttttacat
    16861 cagccacatt atctatttta acacttctaa agtaaagcat catctctttt atttttttta
    16921 ttccatctat gccttttaaa gttactgact ttaagtcctc tttgtagtat ccaaatttat
    16981 tatatgtatc tatcaaagct tcatacaagt tcataccttt tgaagaatag tatgctgcca
    17041 tttcacatat cataagtgaa gatacaacac catccttatc tctagcatga gttccaatta
    17101 agtaaccata gctttcctca tatcccatta taaaacttct atcattgctt tcttcaaacg
    17161 cttttatttt ttctcctata aatttaaatc ctgttaaagt atttaaacaa tctacattat
    17221 aagcctttgc tatttttgca cctagttctg atgtaactat agtttttatt attgtaggat
    17281 tgtttttagg aagtttattt tcttcaacta aactctctaa tatatatcta actaaaagtg
    17341 aaccgacttg atttccattt aagactacat attcaccact tgaatctttt acaacgatac
    17401 ctactctatc acaatcaggg tctgttccta ttatcaaatc agtgccatta ttctttgcca
    17461 tttcaatagc tatattaaac actgactttt cttcaggatt aggatacttt actgttgaaa
    17521 aattagaatc tggcaattct tgctccttaa ctaccattac attttcaaaa ccacattcat
    17581 ttaagacttt tcttactggc ttatttcctg taccatgaat aggtgtatat attattttta
    17641 attttttccc gtattcatct attatatttt gtcttaaaac ttgtttctta acagctttta
    17701 taaactcatc atctacttca ttatctaata tagttattaa atttttagat aaagcttctt
    17761 caaaatctat acttttaata gtactgtaat catcaatctt attaacttct gctattatat
    17821 catttgccat atctatacag atttgaccac catctgagtc atatacttta tatccattgt
    17881 attctttagg attatgactt gctgttatta ctattcccat agcacacttt aaatatctaa
    17941 cagcaaaaga aagttctggt gtaggcttta attcttcaaa aatataagcc tttacaccat
    18001 aagcacttaa agtctttgct acttctatac aaaactccct agacatgtat ctactatcat
    18061 gtgctataac tattcccttt tgcttacctt cttctccttg agttttcatg atataattta
    18121 aaacgcccag tgtcgcacgt ctaacagtat aaatattgat tctattagtt cccgctccta
    18181 ttatacctct taaaccacct gttccaaact ctaaattttt ataaaatcta tcttgtattt
    18241 ctttttcatc atcttttata cttaatagct catttttcgt ttgttcatca aaataaggag
    18301 aatttaacca catctcataa ttattcttat aatccatatt ttcatccttt ctgactctaa
    18361 tttaaaattt caaggtaatt atctatcttc taagtagttt ttgttttcct tttcttaaaa
    18421 attcaaaagc taaatccatc tcttctacct taaataaaac tatcaataca gtatatacag
    18481 aagctccacc taatactcca gctccaactg atataatttc tttaattgtt cctgaaccca
    18541 taaatgcata tagattatta tatatcaata aagtagctac tgccatcaca ccagatgcta
    18601 ccaaactttt tagaccagtc tttataattt tatcccctcc aaaatatcca tttttctttt
    18661 taagagatat aaataataat ataactgtaa ttatgtttga tgtactagtt gatatagcaa
    18721 tacctgcatg acccaatggt cttatcaata ttaaattaag aactatattt attattaaag
    18781 ctatacttcc atttatcatt ggtgtttttg tatctgaaag agagtaaaat gccctatata
    18841 agacatctct tactccacaa gccaataaac ctagtgaata taatctaagc gctatagatg
    18901 taaggtctgt tgataacgca tcaaatttac ccctttggaa taatattcta acaagaggct
    18961 ctgctaatat aatagctcct atagatatag gaagcaccac caatataata caatttgaag
    19021 attttacaat agtgcttata aatccttctt tattatcttt accagacatt tttgctagtt
    19081 taggatatat aactgttatt atagatgtaa caaataatgc cataacaaac tcatttagcc
    19141 tatttgcata gttaagagca gacaactttc catcacccaa agtggttgcc aataatcggt
    19201 caacaaatat atttaactgt ccaacagcta ctcctaccaa cataggcaat actaggttga
    19261 ctaactcttt tatagactca tcctttaaat ttatatatgg cttatacttg taggacttct
    19321 taaacgcaaa tggcagttga aacaatagct gactcgccat agccaataat gctcctgctg
    19381 gcagtatcca tacattttta cctgcactta gtgctattgc tgctattatt ataatattat
    19441 atgggattcc tataagccca ggaattacaa aactgtcatt cacctgtaaa aacgaactaa
    19501 atatcttact tccactcagg aataaaactc ctgaaatcat tattttagta aataatattg
    19561 ctgcttggaa cttagcaggg tcgtttctaa atcctgctgc aaatactgta acaaattgct
    19621 ctgtaaataa aagaccaata atagctatta ctatagccat tatatagcat atatttaaaa
    19681 cattattagt aaattttaag gcaccttcat ctccgagtct tttctttgtc tcaaagtaca
    19741 taggtatata agttgtagca actgcagagc ctatgactgc tatgattaaa ccaggtatat
    19801 ttaatgttaa tacaaatacg tctgcataca tacctgtccc atagaaattt gcaagtacaa
    19861 gttctctaag gaaacctaat acttttgaaa acatagtggc tgccataatc cacaatgctg
    19921 ctttagctgt ttttgacatc ttctttctcc ttatctaatt taaactctaa aagtagaact
    19981 tttaaaactg cttataaaca ttattctaat taagtaaatt taacctatga aaagcttcct
    20041 tataaatcaa aaactatttc gactcatatc taagtgccct aaatgtaatc aatagtaata
    20101 atagtattaa tactatacaa gataagaatg ttagattaaa tatgctctta aatagactat
    20161 aatcacctac tgtataccca acttcaacta catcatttat attgctatct ttcaagaatt
    20221 tatcttgtgc acgacttaag ttatcactac aaatcagtat tggcatattt tcttttgctg
    20281 atactactcc caaagaaata tattcgttta catcagatct actaccaaag tctaatctag
    20341 cagtatagat tttctttata tctttttcat cataaaactt ctttattaaa tcaacatttg
    20401 tacttgactt atttatttgc tttattcctt ctatattttt taagttagaa tcaatctctg
    20461 caataaactc ttttgaatca cctaatgcat atattttttt aagatttttg ccttttaaga
    20521 aatcaatttg ttcctcaaac ccttcatctt tagcccatat tattggcatt ccacttttag
    20581 cggcataaga atatacagat atggcgttct ctaatcctgt tgttgagcta agtaaaaatg
    20641 cttcatcaaa tcctactgtt ctatacattt ctctagctat ctttaaagat gtatcatatc
    20701 tgtctttccc tcttattctt tctactttca ttcccatttt ttctatatta cgttcagcat
    20761 ctttagatac tgcctttaaa ccacctacta tcgtaacctt ctctggacct agttctttta
    20821 gataatttct tgttactcta ccaagatttt tactcttagt tacaactatt ggagcatttt
    20881 ttgcatatgc taaaggtgtt gcacttatac tatctacaat tgactcttca tttacaagaa
    20941 ttacttcttt agaatcctta aatgctcttt tatttaattc tgtcgaaatc tctggtatac
    21001 tcccttctat caattctctt ttgggatttg catctgcaaa tactcctatc gtactagaaa
    21061 tagagattaa caaaactaac gataatatag tagaaatttt tttaatcata agttctcatt
    21121 ccttttcttt tttgtatttt ttgacaataa aaaagctctt tttaaagagc tttttcattg
    21181 attagtaatc taatatttta aaattatcta gctccgtctc ctgtcaatac tactttaaca
    21241 gttttaaaga gtatcttaaa gtcaagtaat atactcctat tttttatata gtaaatatct
    21301 tctttaagtt tttcttcagg agtgatttcg tatccaccat ttacttgtgc ccaacctgta
    21361 agcccaggtt ttatagcgag cctgttgata aatccaggta tttcctcatt aaattgaaca
    21421 gtaaagttcg gtctctctgg tctaggacct attaagccca tgtccccctt taatatatta
    21481 aacagctgtg gaatctcatc aattctagtt ttcctaataa actttccaac cttagttatt
    21541 ctaggatcat ctttttcagc ccattgtgcg ccaaattttt ctgcgtctgt tctcatagat
    21601 ctcaatttat atatatagaa tcttctcccg cattttccta gtctttcttg cttataagtt
    21661 attggtcctt tgtcctctat tttaataaga attccaaata ttgctattaa aggtaaacct
    21721 atcacaagtc ctattataga taaaactaaa tccataattc tttggtacaa gtcgaataaa
    21781 actgttcctt ttactatgct atagtcaatc tcacttgcaa tttctttatc tgcccctgga
    21841 tagcttttaa ttttaggtat ttcacactca tcattagtat atccagacat agttacctcc
    21901 atattggaat aaaaattttt ttaattctta atttcaactt atttctatct tatttttgac
    21961 ttatttctat attatactca ataatgagca gattatcaat acaagaaaca tcattgtaaa
    22021 caatttgatt ctttacaaaa atatatcatc tcttgttata ttttacatta taacaggaga
    22081 tgatatgttt tgctttaata ttttctattt tattttactg tatcttctat tcctattcca
    22141 atttgagttc ccattgtttt tactttttct gcagtattta acttatccat atcgctctta
    22201 ttgtcaataa atgcacattc aacaagtact gctggataat tattggttct taacacatat
    22261 aggtaatctt ttccattatc aagtgttctt gtcttgattc ctctattttt catgttaaat
    22321 ttttctagta ttctttttaa gatatttgat gctgcagtct tagttgttcc accattttta
    22381 tcttttactt tgtaataaat ttccacacca ttaccagctc cattagacgc attgtaatgt
    22441 atacttgtaa ataaatctgg ctttatagta tttgataatg ctgttctttc tcctaaagcc
    22501 attgttttat ctgtatctct agtcataaca acatttatac cttttgaacg taaatattca
    22561 gtagttgcta atgctgtatt aagagtatat ttcttttctt gtgctccacc atttaatcca
    22621 cttgttgccc ctgaatcaga acccccatga cctgggtcta tcactacagt ttttcctgct
    22681 gctgaacctg aattatctgg ttctgttggt gcattgtgtt ttgataaact agatgctata
    22741 gaatttataa cactatcttt cattccatca ccaatttgat atactgtttc tgctgttttt
    22801 tctgataaat ttgtattatg gtatgcagaa acataagttt ttggagttat aagtattgga
    22861 gcttttaatt tagctgctaa aggtcctgct gttattgaat caactattat gtcagatttt
    22921 gcaactaaca ttgcactcaa ctctttatct ggatagaaag tcttaattac atttgcattt
    22981 gtttcatgtc tatctgctcc agaaactcta ttttttgaag taccattttt tgctatttta
    23041 gatatttggt ctataatttt tgatgataaa ctttgactac cacctatgta ataagcttct
    23101 tctaaacctt gtgaagataa ccaattatat gtaccttgtg gtacagaatc tttattagct
    23161 aatattatag gttgttggtc ttcaccagct tttgatgaaa tattaagagc atcatattca
    23221 ccagcatatc catttgctat gtatatttta ttaacatcat gatacttatc tatttctttt
    23281 gctataagta aagatgtttc atgtctatca actccaccaa tacgagtcac atttacattt
    23341 acagcagatt taatttgaga cactgccttt tgtgaaactg aaccatcatc acctataatt
    23401 attacatcac ttggatttag acgttttaac tctgcttttg tactttctgg tatatcatct
    23461 ttatttgcaa gtaatattgg tgcgtcatat gtagatgcaa gtggagtagc tgtaattcca
    23521 tctgctgcta attctccatt aactattaca accttactag acccatcttt ccaaccttgt
    23581 ttacttatct caacagcagt ttcaaatcta gtagctccag caagtttttt tactggtgca
    23641 gaagtttttc tcatatattc atttactgct tttgttgaat ttgcatcact tattataaac
    23701 tcttgtgtaa catcataatc cttaagattt aatcccatag actctaagtc ttctttgttt
    23761 aaaactttct gttcatcttt accatttatt tttatgcttt gaagttccca tacatttaag
    23821 tattctgggt ctttgttata gtttattaca ccttcatatg aatctgtctt ttcattgtaa
    23881 tttaaagtag cttcaagtgg catatcataa cacataaaat ttaatgttat tgaatcagca
    23941 tttaattttt ctttaaattt tacacttgca ttagatttac ttcctaacac tacacttttt
    24001 ctttgtaaat ttatattaat atctgaatta gattctgtat ttgcagtagt attttcagcc
    24061 gctaaagcta catttggtgc caccattgct acagacaaca ttcctgttgc caataatttt
    24121 gttgtttttt tcatcatatc ccccctaatt ttttggttct ttaaatcttt aaatcttttt
    24181 taatacatct taagcttgtt ttatattaca taaattcaat attatgtaaa tcttttttca
    24241 ttatagcaca aaaaattaca aattgtaaac tttttgtaat cttttatggc atggttttta
    24301 aattttatcg aggagaaatt ttgattatat ttgatagttt ctgtatattt gaacaaatca
    24361 aagactttat catctaaaaa tattgcacta aattttaata aatatttttt tcttgtacat
    24421 tatagacatt actataaccc agaataggac atacaataaa gaaaatataa gagaatccca
    24481 agttgtacct gcccaaggtg ttataagttc atatgtagtc cactctacca aggtagttgg
    24541 ataatcgact ttgttcgtaa gttttggaac attccaaaaa acatgactca atatctcaag
    24601 gcacatatat gtaaaaatgg ggctacttcc aagtgctatt attggggtaa atattttact
    24661 tttatttttg atgtcgcata taaaataaaa tattgacagt aatataccat aactgccagc
    24721 cattaaaagt acaaatgagc tagaccataa tcttttatta aatggaaaat actgattaaa
    24781 gataaaagca cctattagca ggattatgct cataaccagt attttaaaga attttttata
    24841 ttctcctagg ttttctttta aaagtacaca tcccatagtg catcccaaca tacctgaaga
    24901 aattgctact atactagtta aaaaaccatc tggttcaaat tccggcttgt acaaatgtcc
    24961 tttgaagaaa tgtaaatcca ctaactgtgc aagagaacct tctagctcaa atccataagg
    25021 ttttgctaag atgtagtacc caactattat aaatgtagct atacatagaa aagtaattat
    25081 tgtagcagta cttccaacat ttaatttttt tagcagtaaa tatgctaaac tagtcacaaa
    25141 atagactaat cccatacgtt gaagtactcc aagtattctt acagtatcta aatcaggatt
    25201 tcctaggtaa tttaagaaaa atccaaataa tatcaataag atacttctct tgaatatact
    25261 aagtattata ctcagtgtgg atttattatt ttttagttta ctatttattg atattggaat
    25321 tgtaacacct aaagatataa caaaaaaagg aaatgcaaaa tctgcaagtg ttacaccatg
    25381 ccatacagca tgtcttaatt gaggatacat tctcatccaa gtcccaggat tattgcagac
    25441 tatcatgagg gctattgata gccctcttat tatatctatt gatttaactc tactatttat
    25501 aagtttagaa tctacagcat tatttgaaat t
//
LOCUS       DAEMGG010000048        25189 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEMGG010000048 DAEMGG010000000
VERSION     DAEMGG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.048"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(21..1406)
                     /locus_tag="KPK24_001872"
     CDS             complement(21..1406)
                     /locus_tag="KPK24_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2299546.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALVEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(1514..1657)
                     /locus_tag="KPK24_001873"
     CDS             complement(1514..1657)
                     /locus_tag="KPK24_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299547.1"
                     /translation="MYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRF
                     EIN"
     gene            complement(1852..2274)
                     /locus_tag="KPK24_001874"
     CDS             complement(1852..2274)
                     /locus_tag="KPK24_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG2299548.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            2752..5655
                     /locus_tag="KPK24_001875"
     CDS             2752..5655
                     /locus_tag="KPK24_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2299549.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISVSEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            5688..6134
                     /locus_tag="KPK24_001876"
     CDS             5688..6134
                     /locus_tag="KPK24_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2299550.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            6257..6559
                     /locus_tag="KPK24_001877"
     CDS             6257..6559
                     /locus_tag="KPK24_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2299551.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            6740..8146
                     /locus_tag="KPK24_001878"
     CDS             6740..8146
                     /locus_tag="KPK24_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG2299552.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            8490..9548
                     /locus_tag="KPK24_001879"
     CDS             8490..9548
                     /locus_tag="KPK24_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2299553.1"
                     /translation="MGDKMRASVLYSVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            9647..10333
                     /gene="araD"
                     /locus_tag="KPK24_001880"
     CDS             9647..10333
                     /gene="araD"
                     /locus_tag="KPK24_001880"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG2299554.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            10351..10998
                     /locus_tag="KPK24_001881"
     CDS             10351..10998
                     /locus_tag="KPK24_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG2299555.1"
                     /translation="MLDSLKGKIVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(11171..12538)
                     /locus_tag="KPK24_001882"
     CDS             complement(11171..12538)
                     /locus_tag="KPK24_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG2299556.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(12728..12816)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            13244..15364
                     /locus_tag="KPK24_001883"
     CDS             13244..15364
                     /locus_tag="KPK24_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG2299557.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKTIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKTCNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNILEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            15468..16721
                     /locus_tag="KPK24_001884"
     CDS             15468..16721
                     /locus_tag="KPK24_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG2299558.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSKMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(16874..17935)
                     /gene="ytvI"
                     /locus_tag="KPK24_001885"
     CDS             complement(16874..17935)
                     /gene="ytvI"
                     /locus_tag="KPK24_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG2299559.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            18209..18949
                     /locus_tag="KPK24_001886"
     CDS             18209..18949
                     /locus_tag="KPK24_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG2299560.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            18961..20739
                     /locus_tag="KPK24_001887"
     CDS             18961..20739
                     /locus_tag="KPK24_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2299561.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            20906..21823
                     /gene="pfkB"
                     /locus_tag="KPK24_001888"
     CDS             20906..21823
                     /gene="pfkB"
                     /locus_tag="KPK24_001888"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG2299562.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            21860..23779
                     /locus_tag="KPK24_001889"
     CDS             21860..23779
                     /locus_tag="KPK24_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2299563.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            24275..>25189
                     /locus_tag="KPK24_001890"
     CDS             24275..>25189
                     /locus_tag="KPK24_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2299564.1"
                     /translation="MIFTIIFYKKGRLCLLNLQSKWVNFLKPTYVSDEIKRKKLQVII
                     FFSITYGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFY
                     SSVILFFIIYFFIFIIESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNF
                     KIGILLFFYFLFSQIIFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSF
                     LQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLG
                     YVYMKTKNIWFCSIMHVLINSLGLIFNDS"
BASE COUNT         9872 a         2780 c         4215 g         8322 t
ORIGIN      
        1 aaatattgtg tgcaatacaa ttattttttc tcttctaata gtctttttaa tacgctcaaa
       61 ccagctttaa gttcttctgt actttctgta gcacatacag ctattcttat tgcagaaatt
      121 ggtttttctt ttccaacagc aaaacgttca gaagcataga cttgtacacc ttctttaagt
      181 gcttgtatct caaatttttc tgccattatt ccatcaggta gaatcaacca tctaaatata
      241 cattcttcat ttcccaaaag attatatcca gataaatatt gattaataat cttgtttctt
      301 cttcttgcag atttacggcg tgcattaatt aatttatcgg cttcacctga tgtaatcatg
      361 cgataagcaa tttctgttaa aaaataagat tgagatagat taatgttgta taatgcgctt
      421 gataatgcct ctcgatactg tttaggtgta gagatatatg ctagccgtaa actaggtgca
      481 ataatttttg ataaactcgt aatataaatt gtttgatttg gcagatagga agccactgga
      541 tttaaatgtg tttcattaag taaactatga atagcatcct caataacaat taaattgtat
      601 ttagatgcaa tatttgcaat catttttcgt ccattttgtg acattatatg tgtagttgga
      661 ttttgataat ctggtattat gtatagacct ttaatatttt catttttaca agcatataat
      721 agtccttctt cactaatctc attatcttct tgtttaattg gaattagttg aatcccaagc
      781 attttagctg ctgtctttat tccaggataa gttaatgggt ctacacctat tctatctcca
      841 tgttgaaaca aaccagctaa aatagcaaca attgcatttt gtccaccact tgcaggaagt
      901 aagctatctg atgtagtttc aagcccagct ttataaatta atttggcaat agcttctttt
      961 tgccatttag caccatctct ttgtccatat tggaatatag ttttaaaatc tatttcctta
     1021 attatgtgtt taaacaaagt attcatttct tctagcgtga aatctggatt cattgtgccc
     1081 atttcaataa atgtaatttt attattactg gacatcaaga atagatttga acgtgtatca
     1141 taagatacaa aagttccact tcctgtaact ccacttatta gacctttttt ttcacaaatt
     1201 ttaaaagctc tagagatagt actcacatta acatctaaga aatctgccaa ttcacgttgg
     1261 ggtggtaatt tagtacctgg taataaaaat ccattgttga tatcttgttc tagttgttct
     1321 acaagcgcct tatataatat ttgccctttt gaaggtctct ttggtttcca gttcatagga
     1381 taattttcaa aagaattaat tggcataagt tttcacctca cattattaat ttgatttttt
     1441 ctttatctaa aacaataaat tctaaagata caaataaaaa ctgataggaa ataattctat
     1501 cagtttttgt cttttaattg atttcaaatc ttcctataat ttttatacct tctttatcat
     1561 aaagagggat tgttttttta aatatattaa aatttctttt tcttgtatat aaaacagctt
     1621 cttctggtgt ctttggttgg ttatcacaat cgtacaaatt tctttttttt aactaaaagc
     1681 aataatacat aaaattatta ttgctaatga tactcttaat gtggtatata gtcatagtgt
     1741 cacatgtcta tcaatagttt tttcagtaat tattgtaacg aatagtagga ctcataaaat
     1801 acttagaaat atgtttttag ataaatttta aatatataat ctaacttata tctataaatt
     1861 aacactatta ttctccaatt ccatttctag tttttttata aatttattaa attcttctgg
     1921 ttgccttttt ccaaacaata ctcttacaag agctgaattg atttttccaa atatattcct
     1981 cttgctctca ctttcaataa aaataagttt tgtattttct aagtcaatat gtactaattc
     2041 atatctagta atagtacttt ctcttttatt gcttgttttt atctcatata ctctattttt
     2101 ttcgaaatta gttatttcaa ccttaaatct tttattattt ttttgctgta caaatgttcc
     2161 tactgctttt ttctcattaa aattaggaaa atttagtctt gcagattttg taaatatatt
     2221 aaatatgtct tttgcagaat attttaataa aacttcagtt ttaaatgtat tcatatcgtt
     2281 ttcccttctg tatataaatt taatatttat aagttaattt ttaagttaaa atagtataat
     2341 atgccatatt aaatcatgtt aaaactattt atatagctaa aatcttacct atataagtat
     2401 gtatactatc atataaaaat aaaatcttaa aatagaaagg gttaaaaaca cagtttatta
     2461 taatttttat tgttgagtag atacgtacta ttatatatga ttgagcaagt ttttcaatca
     2521 taaatttata aagtattttg cattaaaatt taaacaaaga attaaattat ttaagattat
     2581 gaataactaa tacatttagt aaattactaa atatacaaaa tgtaaatact tatcaaaatg
     2641 tataaatact tatgacatct agttacttac agggtttaag tagaaaaaaa tatcaaagta
     2701 agtatggcat gatagttgct tatatattta tcaagaaagg agttattaag catggagttg
     2761 agagataaac ttgtaaacat cataaataca gaaaataaaa aagatccatt aacagatgtt
     2821 caaatagcaa agtttttaag tacaactaga gagaacataa ctaatttgag aaaagaactt
     2881 aatataagta attcaaggca aagaagatat ccttatttaa aatcagcaat ttcggcaata
     2941 ttacaaaaga ataagaatat ttctgtatca gaaattacaa gggaattgat gacagaaggc
     3001 tttaatatat ctagaagagt tgtggaggaa cttttaccta aagaaagttt agaaatggat
     3061 gtagtagagg aatctgaaga agaatcgaat gacccatttg aaactctgat aggtaataaa
     3121 gggagtctta gaaatgctgt agagcaggct aaatctgcta tattgtatcc accaaaaggg
     3181 ttaccaacgt tgattattgg ggaaagtgga gttggtaaga gtttattttc tagacatatg
     3241 tatgagtttg caaagcaaaa aaagatagta aaagaaagct ccaattttgt tgtatttaac
     3301 tgtgctgatt atagtgacaa tccacaatta ttattatctt tgttatttgg atataaaaaa
     3361 ggtgcattta caggtgctga atatgataca ccaggtcttg tagaagaagc tgatgaagga
     3421 gttttatttt tggatgaaat acatagattg cctccaaaag gccaagaaat actattttct
     3481 attttagata ggggaaagtt taggagatta ggtgaaacta atgctgaaag aaaagtaagt
     3541 attatgttga ttggggcaac tactgaaaat gtagagacta atttattatt aacattcaga
     3601 agaaggatac caatgctaat aactttgcca ccattacatg atagattact aaaagagaaa
     3661 atagacctta tatacaatat ctttcaacaa gagtgtaata gaataaatgc aaaaatattt
     3721 gtagataaaa atgtgattga aatacttgca ctaaaaaagt ttagtggaaa tataggacaa
     3781 ttacaaaata tgattcaggt tctttgtgca agagctttta tgaagtttat aaattccaaa
     3841 gatgaagata gtgaatcgat agtagtagta gatataaatg aagttttaaa gcttaaagat
     3901 tcttttaaag atgctgcctt tcaagagatt gaatatacag agataagaaa atatttgaaa
     3961 aatgctatat ttataccatt taatttagaa gaatcatcag taaaaggaag tcttttaagt
     4021 gatgaatata tattaccaga ggacatatac aaaacaattg aaaagaaata ttatgaccta
     4081 aaatccctag acattgaaga tatagatata gagaatattt tatggacttt tattttaaat
     4141 agatttgctg acttaaagtt taatctcgat agtaatgaag aaattttttc aatgagtgat
     4201 ttaaatagtt ttgtaagtga tagtttagtt aaactggtaa aagaacttat gaatgatata
     4261 gttgaaaaaa atatgaattt agaagtaaat aaaaatatgt tcaaatatct agcaatacac
     4321 ttagaagaag ctataaaaag aattaagcta aatcaaaaga taataaatgt aaatttagaa
     4381 aaaataaaga ttgacttttc aaaagaatat gaaattagta aaagttttgc aataaaaatt
     4441 gaggaatcaa ttggtattaa gattccagat gatgaaattg gatttataac actctacata
     4501 aagtctgctc taaaaaatga agtgaaaaaa gataaagtag ggttaataat aatttctcat
     4561 ggacgtatag ctacagagac tgtaaatgta gttaaagaac ttttaggtgt aaagtttcct
     4621 gtggctatag atatgccttt agatgaaaaa ccaataaata tatacaataa ggcagtagaa
     4681 ctgtcaaaaa taatagacca agggaaaggc atattgtttt tggtggatat tggttcactt
     4741 acaaacatag gtcaaatagt aaataagaga accaatataa gcactaaaac tattgataga
     4801 gtagacctcc taatggcact agaagcaact agaaaagttt ctatgggaga ggaagaactt
     4861 gatgaaatat ttttttcttt gctaaaagat aggatgggat ataactatga attagaaaaa
     4921 cacatagata aatcaaatgc aattgtaaca ttatgtctta ctggagaagg aactgctaag
     4981 tatataagta aaacgttgga agaaaagtat gataacacta aatgttatca aatgagtgca
     5041 ttggatgaaa atttattttg taaaattgaa aatctaaaag agacaaacaa tatattagct
     5101 attgttggga caataaaccc taagatacca ggtataaatt ttatacccta taataaagaa
     5161 cttttcaaaa atttggatat ctatctatca aaatcaaaac aacaagatga cagaccagtt
     5221 aaatcgtatg aaagaatgct tgatgaggat ttagtgattt ttgaaccaga tatatatttt
     5281 aaaaaggatt tattagaata tgtatgttca attcttataa ataaagaata tgtagaaaaa
     5341 gattacttag attctgtttt acatagagaa gaaatgcttc caacatattc taaaggagct
     5401 ataggtgtgc ctcatggaga ttcttctact gtaaatagta caaggtttgt ttttgttaaa
     5461 ctaaagaatc ctattgattg gggtgtaggt aatgtaaatt tcatatttat gccagttttt
     5521 caggctaatg ataaagagat tgttaaaaat gttcttgaga tactaaaaga tgcagaattt
     5581 atgaacaatg ccaataagtg ttttgataaa gatgctttta gaaggattat atttgataaa
     5641 tttaaacatc tgtagccata gcaaaaaaat tgagaggagt tagagtaatg aatgtagatt
     5701 taatatcatt gggtttagaa tctaatgatt atgaaagtgt tattgaagaa ttaggaagta
     5761 taatgtgtaa aaaagaatat gtaaaagaaa catacataaa tgctgtcttg gaaagagaaa
     5821 ggacattacc aacaggtcta gatattggag aaatgtgtgt tgctattcca catacagatt
     5881 caaagcatgt aaatgagtct aatgtagctg tagggatatt gaaaaatcct gtaaagttta
     5941 attcaatgat agacccaaaa gatagacttg atgtagaact tgtattttta ttagcagtaa
     6001 agaacccaga ttcacaagta aaactgttaa aggatttaat gtcagttttt caaaatataa
     6061 agttattaaa aaatataaaa aacgcatcaa caaaagaaga agtagctaaa ttattggatt
     6121 ttatagaaat ttaatgtagg agaattttat aaataaaaaa ttaaaattta aacaatcaaa
     6181 gtaagtatta tttttttaat aggtttaatc ttatataaaa taaaaattta gagtgaaaaa
     6241 ttaggaggaa attattatga aacaatttaa tgtattatca gtttgtggtt cagggacagt
     6301 tacatcatct atggtagcag gtaaattaaa agaagtacta ggtaataagg ggatttctat
     6361 tactacaaca gaagctagac caacagaagc actaaattta gcacaatcag gaagatttga
     6421 ctttataaca tttacgagtc cacttcaaac aggcgattat ggaataccaa ctataaatgc
     6481 atttgcttgc ctaactggta taggggaaga ggcattcttt gaagaagttt tgactgttat
     6541 tgaagggata gataagtaag ttttggagac gtgaatttat agtatttaaa tcaatgattt
     6601 agatactata ataagctaat attaactaaa tatttgtgga aaaaacattt tatatgttgt
     6661 cataaattaa aaaattataa aaatagtcac ttaagtatta aattaagctt ataaatattt
     6721 ataagtagga gggaaaaaga tgttagaatt tatgaaatct ataatcgata catttggttc
     6781 tgcaattata gttccaatta taatattcat aatagcaaag atatttaaag taactactaa
     6841 gaaatcgttt ctttctgctg tgtatgcagg agttgcttta caaggtttta cactgatttt
     6901 aaattcattt acaccaataa taacaccagt tatcaatagg atggttgaga gtacaggagt
     6961 aaacctacca gtttttgatg ttggttggca agcaacatca ttggttgcat tttcaacaag
     7021 tgcaggtatg atatatttag gtcttggtat attattacaa acaattttat ttttgataaa
     7081 atggacagac gtgtttcaac catcagattt atggaataac tattcttata tggtatgggg
     7141 agcaatggtt ataggagtaa caggaaattt tcctttaggt atagcatgta tggttttatt
     7201 aaacctatat agtttattaa tatcagagtt agttgcaaag agatggtcta gttactatag
     7261 atatcctaat tgtacaataa tagctatgca taatgttgaa gcttctgtgt ttgcagtatt
     7321 tgcagaccct atatacaata aattgggatt aaataaaata aaattaaatc caaaagaatt
     7381 agagaagaaa ttagggtttt taggtgaacc aattacttta ggtctttttt taggtatgtt
     7441 tataggtata ttaggtaata tgacgagaat aaatactatg gaagcttggg gagaaataat
     7501 gaaagtaggt atatctacaa gtgcagtgat ggcaattttc cctaaagtag caagtatgtt
     7561 tgctcaagca tttgcaccta taacagaagc agctagaaag attatgcaaa aagctggaaa
     7621 ccgtgagtgg tacatagcag taaatgatgc tgttgggtat ggtgaacctg caactttaat
     7681 atcaggtctt atattaattc caataatgct tgtaatagct atggtacttc ctggtaataa
     7741 ggtattacca gttgtggatt tattggcaat tccatatatg gttcaaggtt tagttgccat
     7801 acataatggt aatataccaa aagtattggt ttcaggtata atttggtttg gattaggatt
     7861 atatgtttgt acatctacag caccattatt tacagatatg gctacaaata taggtgtagc
     7921 tatacctgca ggagcaatgt taataacaag ctttaatata ctaggaaaac ctttgatggg
     7981 attagttttc tttgcatttc taagtgcaaa tccaatttat ataggtcttt cagttgtaat
     8041 ttactttgtt ctatgggcat tgtttagaaa gaacaaaaca agtattcttg attatttaga
     8101 aaaacaagca cttaaaaatg tagaagaaga gcctgtagca gtttaagttt ataaaaatga
     8161 aataagaata attaaaggag tttataattg attttatata agtatcagat aatacctgag
     8221 aaatatttaa atgaaatata gacttctttt tatttgataa tatttgtaat tataatgcat
     8281 tgtatcttta tagttgagtt ttctgtataa cctgctattt aaaacatact cttttatatt
     8341 tatacagtaa tgtggtttaa ttttataaaa tgtaatctct actggtaaaa gactttgtat
     8401 ataaggcatt tgcctgaata aaccatttat cttttgtatg gcatagatat tgctatatag
     8461 aagtatatag catgaaagga gatatttata tgggagataa aatgagagca tcagtattgt
     8521 atagtgtagg tgatgtcaga tatgaaatgg tagatatacc agaaataaca gatacgcaag
     8581 tgcttgtaaa tgtaaaatat gttgggatat gtggttcaga tttacctagg tctatggtaa
     8641 gtggtttgag tggaaataca aaatatccat tgattttagg acatgaattt tctggcgaag
     8701 ttgttaaaat tggagaaaaa gtaaagcata ttaatgtagg agatagagta gctgttgcac
     8761 cattagtacc ttgtggaaaa tgtgattatt gtaatgaagg taattttggc ttgtgtgatg
     8821 attacaatat aattggaaca agagtaaatg gagcgtttgc agaatatgta agagttccag
     8881 aagaacatat tttaaaacta ccagatacat tggattatga aactgctgcc ggaattgaac
     8941 cagcaactat agcttatcat gggatttcaa agtctaatat tagagtagga gatagtgtag
     9001 ttgtgttagg atgtggtcca ataggtcagt ttgttataca atgggcaaag gtgtttggtg
     9061 catctaagat tatagcagtt gatatttttg atgaaaaatt agaactttct aaacttctag
     9121 gagcaaacta tatacttaac tcaaaagaag taaatgtaat caaagaaata aaaaaaataa
     9181 caaatggtgg tgctgatgtt gtaatagaga ctgctggtag ccgatttaca caagaacaat
     9241 ctctatttgt agcaaaaaag aggggaaata tagtgtttgt aggtatatca cacacagaat
     9301 taccacttag tgcagacgct actgagtgta tattacgtgg agaacttacc ttgaaaggtt
     9361 cttggaattc atatacaagc ccttatcctg gaagagcatg gactgcaaca ttggatttta
     9421 tgggaaaagg tgacattatt tttaaaccca tgatttcaca taaaataggt cttaatgaag
     9481 tgggtgactt tttaagtaaa atgagcaaga gagaaattaa tttcaataaa atcctagtag
     9541 aaatataaaa atttaataaa aaattctaaa agcaattaaa ttttataact atgacattaa
     9601 ataaaataaa aataattttc ttaaataaaa tataggagga agaaaagtgt tagaaaattt
     9661 aaaagaagaa gtattaaaag caaacttaga attacctaag aaaaagctaa ttacttatac
     9721 ttggggaaat gtaagtggaa tagatagaga aaaaggatta gtggtgataa agccaagtgg
     9781 tgtagagtat agtaatatga ctgtagaaga tatggttgta gttgatttag agggaaatat
     9841 agttgatggc aaattaagac cttcttcaga taccccgact catctagttt tatataaaga
     9901 gtttgaagaa ttggggggaa tagtacatac acattcaagt tgggcaacaa tttgggctca
     9961 aataggaaaa tctataccat cttgtggtac tactcatgca gattattttt atggaagtat
    10021 accatgtacc agaaaaatga ctagagaaga aatttgcaat gaatacgaaa aggaaactgg
    10081 aaatgtaatc attgaagaat ttatgggaag aaatccaatt cattgtccag gaataattgt
    10141 aaatgaccac ggacctttta catggggaaa agatgcaaat gaggctgtac acaatgcagt
    10201 agttttagaa gaagtcgcaa agatggctta ttatacagag ttaatgagtc ctgataatat
    10261 tatggataag gttcttatga ataagcattt ttcaagaaaa catggaaaaa atgcatacta
    10321 tggtcaaaaa tgattatctg gaggtgaatt atgctagact ctttaaaagg taaaattgtg
    10381 gaaatagcaa ttaaagcaga gcattatggt ttatgtaaag aaaagactgg taattttagt
    10441 attagagata aggatactgg atatatactt ataaccccat caggtgtagg aagagaagaa
    10501 ttaaaagttg aacatatttg tgtattggat ttggatggaa atgtaataga agttgagaaa
    10561 ggtattaagc caagtagtga ggtattgatg catattgaaa tatacaaaac aagagaaaat
    10621 ataaatgcca tatcacatac tcattcttta tatgcaacag cattttcagt agcaaagaaa
    10681 ccaattatac caattgtata tgaatcagtt aattatggcg gttatgtaga tattgctcca
    10741 tatgaaagac caggaacttt aaaattagct aagagtgttt caaaattact gatagataca
    10801 gatgcatgct tattagaaag acatgggttg acaactgtag gtaatgattt agacgaagca
    10861 ttattaaaat caagatatgt ggaagaggta gcagaaatat actataggtc tttagtatta
    10921 aatcaattta aagaacttga agtagtagat gttgatgagt ttaaatcttg gaaataccca
    10981 gaagaaataa agttttaatc taaataattt tcattgaagt ggttaattaa aatatatgta
    11041 ttatttatat caattactat gttttattat gttaattacc gtattttgca aatatattta
    11101 ttacattttt tcaaaaaaat atagaatccg aaagccatat aaaattgact tgggattcta
    11161 tactttttat ttatgcattt ttctttatct gattttctgg agaatttata tgttcaaaat
    11221 attttttagt ttcagatact attataggtg ataatgcaag taaagctata aggttaggta
    11281 ttgccataag cccatttaca atatcagcaa ttatccaaac aacttctaac tttaagaaag
    11341 cacctaataa caccatagcc acaaatacag ttctataccc attcatgcct tttacaccaa
    11401 acagaaattc aaaacatctt tctccataat aactccatcc caaaattgta gtaaatgcaa
    11461 ataatgttaa actaactgta agtaatattg gtccaaatgt aggaattgct ccgttaaaag
    11521 ctgcttgtgt cataacagct ccatttaggt ctgaacacca cactcctgaa ataacaagag
    11581 agaatccagt aagagtacag attataatag tatctataaa agtacctgtc atagatataa
    11641 gaccttgttc cgctggccat tcagttttag cagcagcagc agctattgga gcactaccta
    11701 gaccagactc atttgaaaat acaccacgag cgataccatt tctaattgcc agtgacatag
    11761 ttgctcctaa aaaaccacca gtagcagcag taggtgtaaa tgcactttta attattaaca
    11821 taaaagtttc agggatttta ttccaaaaag atattaaaat gattccacag attacaacat
    11881 atataactgc cataaaaggt actattttag tagctacatt agatatactt ttaagtcctc
    11941 caaatacaac caaagctaca aatactgcta atacaacccc tgtaattcta gggtctatcc
    12001 cgaaactatt atttattgca tctgttattg aatttacttg agtaaaagta ccgattccta
    12061 aaagtgcaac tagtatacca cttatagcga aaaatattgc aagaggtctc catttttctc
    12121 ccataccatt tactatgtaa tacattggcc caccagacac ttggccattt ttatctttag
    12181 ttctgtattt tattgcaaga acaccttctg aatacttagt agccattcca aagaatgctg
    12241 ctatccacat ccaaaaaagt gcaccaggtc caccagcttt gatagctgta gcaacaccaa
    12301 caatattacc agtaccaact gtagcagcta gagcagtaca taaggctgca aaacttgaaa
    12361 catcaccact acctttgttt tcagcagaga atattaactt aagagctctt ggcagtttag
    12421 ttatttgaac aactccaagc ttaacagtaa gtagtatacc agtaccaact aatagtacca
    12481 gtaggggtgg tccccaaata aaagcatcaa ctttgtttaa aatatcaatt agattcataa
    12541 aaacactccc tcattttttt tgctttacat aatcaataga atttttaatg aagtaattga
    12601 caggaaataa gtataattta ttaataattt ctagtgtaat aaaaaaagct aaatctaaaa
    12661 aagattcagc tctggatgta cactaattag caaccaaaat aaaagtatta gtaaatactg
    12721 tttttggtag ataatccctg tccttttgcc tgagagattg agcataaaag ccttgcccct
    12781 tcggcgccca taatgggtct ctccagagtt ccgtaaattt tacagtccta cttttaaaag
    12841 tacctgagag tgttactcct tcggtgtgct atatagcaac tcttctataa aatcttaacc
    12901 ggatattgat ttgtcatagc ttattgtatt aataatatta atataagttt ttatgttttg
    12961 tcaactaaaa aattaaaaaa caaagaaaaa atataaaaat gtaagcctat ttacgaatta
    13021 taaacttaga aagatgagta atatacgtga aaatgaattt tgtaatacac atacaaatga
    13081 agtgtaaaat aagttctaaa taaaaaaata ataaaatctt aaataattaa ttagatatga
    13141 ttaaattatg gtaatattat agttacgata ttaagaaaag ttctatttag ttagatttta
    13201 agcgtaatat gagattatgt atagagaaag gatgtatttt tagatgggaa aaatattagt
    13261 gctagcagaa aaaccttctg ttggaagaga tatagcaaaa gttttagggt caaaaaatga
    13321 aaaaaatggt tatatagagg ggccaaagta tgtgataact tgggcactag gtcatttggt
    13381 tacacttgct gaccccgaaa gttatggaga aagatataaa tcatggagct tagaagacct
    13441 gccaatactt cccaagcacc ttaagactgt tgtaataaaa aagagtggaa agcagtttaa
    13501 tacagttaaa tcgcaaatga atagaaatga tatagatgaa atagttatag caacagatgc
    13561 aggtcgtgaa ggagaacttg tagcaaggtg gataatagaa aaatcacagg taaaaaaaac
    13621 tataaaacgt ctttggattt cttcaagtac agacaaagca ataaaagacg gatttgcaaa
    13681 attaagaagt ggaaaagaat atgaaaattt gtattactca gcaatagcac gagcagaagc
    13741 tgattggatt attggaataa atgcaaccag agctttgaca acaaagtaca atgcacagct
    13801 ttcttgtgga agagtacaaa ctcctactct tgcaatgttg cttaaaagag aagaagaaat
    13861 aagaaatttc aaacctaaag agtattatgg attagaatta attgctacaa agggaaattc
    13921 agatataaaa tttatttgga atgataaaaa caataattct tcaactttta gtaaagaaaa
    13981 gatagaatcc acattaaaaa aagtaaaagg tgtagacata aaaatatctg aagtaaacaa
    14041 gtcatataag aaaaaatatt cacctgcact ctatgattta acagaattac aaagagatgc
    14101 aaataaaatg tttggatttt cagcaaaaga aactttatct ataatgcaaa aactgtatga
    14161 acatcataaa gtacttactt atcctagaac tgattctaga tatttaacat cagatattgt
    14221 tggaacatta aaagatagaa taaaagctgt aaatattagt gattattcaa agacttgtaa
    14281 taaattatta aaaagcaata ttatagctaa taaatcgttt gttgacaatt caaaggtaac
    14341 agaccatcat gcaataatac caacagaaca gaaggtattc atgagtgatt ttagtgacaa
    14401 agagcgaaaa atatataatc ttgtagtaaa aagattttta gcagtactat ctcctccttt
    14461 tgaatatgag cagacaacta taagtgcaac tgtagaagga gaaatattta aggctaaggg
    14521 aaacaaagta ataaatttag gttggaaaga aagttataaa gatattgaag atgaagatga
    14581 gtatgaaagt ataccaaata ttttagaaaa agatgtactt aaggtcaagg gattaaagat
    14641 aacttcagga aaaacaaatc caccaccata cttaaatgag ggaacattat tgactgctat
    14701 ggaaaagaat ggtctaggga cagttgctac aagagcagat ataatagaaa aattattcaa
    14761 ttcattttta atggaaaaga aaaataaaga tatatatata acatcaaaag gaagacaatt
    14821 attagattta gttccagtag acttaaagac accagaactt acagcttcat gggaaaagaa
    14881 attattggat atttcaaagg gaaaacttag taaaaataca ttcataaatg acatgaaaga
    14941 ttattcaaaa gatattgtac tagaagtaaa aaatagtgat aataaattta aacatgataa
    15001 cttaactaaa aatagatgtc cagaatgtgg taaatttatg ttagaggtaa atggtaaaaa
    15061 aggtaagatg ttgatttgtg aagatagaga gtgtaatact agaaaaacta tatcacaagt
    15121 aaccaattca agatgtccta actgccataa aaaattagaa cttcgtggag aaggtgaggg
    15181 gagaatcttc acttgtagtt gcggatacag agaaaagttg tcgtctttca acaagagaaa
    15241 gcaagaagaa aaacagaagg catcaaaaaa agatgttaat caatatttaa aaaatcaaaa
    15301 taaaaatcaa gagacattta ataatccatt tgcagaagca ttagctaagc taaaaaacaa
    15361 ataattttaa agttattatt atacacaatt ataactttaa ctgtatatta gttatatgta
    15421 ctgatataat atagtgtata gtttaggcat gaaagtaggg ctgttaaatg gatttaagtg
    15481 gcatatttaa atactattgt aaagagtgtg aaaatacatg gaataattcg agtgttgaat
    15541 tatttgagaa tatagaaacg tatagtaaag attcacaaaa aaagagggaa aaagaattag
    15601 ataaattgct aaatacaata tcagttcatt tagagaggta tccaagtgat gctgtattga
    15661 gaaaaatgtg ggtaaaaaag ggcgaggttt tcttacaaaa gacattggaa aaagaaaata
    15721 tttttaagtt agaaaaaatg gatgtagagg atagaaaaaa atttttagat ataacaaaac
    15781 agtttattag agatgctaga aaatttgatg atgatttacc tataggtgat attatgcaag
    15841 ctatgaggaa tgtatggatt tcaaatgcat tacaattatt atttggtaaa gaagtatatt
    15901 attcaaaagc taactttgca tatagtatgt tatatccata tactgataat tatttagaca
    15961 atacaaatat agataaaaat gataagattt tatttaataa ctggttagaa aaaaggctcc
    16021 tgggagaaca cattaaatct aaggattatc atgaaagtaa agtatctaaa atgatagatt
    16081 atattgaaag tgtataccct agagaaaagt ttacagaagt ttatgaatcg ttattattaa
    16141 tatttaaaag tcaagtaaat agtttaaaac aacatggtaa ggaaaatcat ttgtgtaaag
    16201 aagatttatt atccatttct attgaaaaag gaggttcatc cgttttagta gatggatatt
    16261 taataagtgg attgatgaca aaggaagaaa tagagttttg tataggatat ggatttttat
    16321 tacaaatatc tgatgattta caagatataa aagaagattt aaaatacaac cataaaacta
    16381 ttattacaga gatgtcaaaa gagggtactt tagataaagt tgtaaataaa ctaataaatt
    16441 ttactattga gttaatagat agttttaaaa ttaataataa aaataaatct gtaataacta
    16501 tgataaagaa tgattgctta atgttaattt tattttctgt agtttataat gctgaatttt
    16561 tttctgtagg atatataaaa gaagtagaga aatttattcc atatacaata gattattcat
    16621 tagagattga agaaaaaata aaagaaaaat ttaaaaatat agatgtttta aataatgaaa
    16681 atgaatataa agaaatgatt gatattattt gtgcagagta gaaaaaaagg gagtgtctta
    16741 gaatagactt tttagtttat gaggcactct ttttgtataa aatcaaatgt attttcatca
    16801 ttttaataat taaaaagagc ttatccctat ttgagaaagc cctttttaat ttaataaaaa
    16861 tgtcaaggtt aaactattta aatgcagtaa taatcccatt atcatgtaag ttttttatga
    16921 ttgtagctgc gatagggaat ccaaataatc cagctattcc aaatagttga gctccaacaa
    16981 acatacacat taatgttatt aaaggatata ggccaatttg gtgtcctact acctttggtt
    17041 ctaatatttg tctaacaacg gttataatta tgtatagtat taaaagtcct attgctaaat
    17101 taatatttcc attaatgagt gagataatca tccaaggtgt caaaatactt cctgtaccca
    17161 agactggcaa aatatcaaca agtgctatga tgacagatat agtaaatgca ttttcaacct
    17221 ttaggatagt aagtccaata agtagttcta tgaatgtcac tgatagtagt attgcatagg
    17281 cttttagtag ttttaaaaga gcattaatac tgctattttt tatatttatt atcataaatt
    17341 gacttttttc tggaaattgt tttaagataa agtttactat tttttgatag tcaaaagtaa
    17401 agaaaaaaga agcaattaaa gtaataataa gttttaacat aaatgcaggt gttttacttg
    17461 caatactagc aatagcattg agtgcatttg ttgaaataga agctactaaa gacatcatag
    17521 attgacttat attatcccaa ccaagtatta atgctaggtt aacattaggt gtactagcat
    17581 ctatctttga aaataaagta tttagcgttg gttgtatact tgatttatat atttcaggaa
    17641 gtctaaaaaa tacatcactt acacttgcaa atatcttcac accaaagcta actaataaaa
    17701 atatacacaa cccataaaaa attaaaagta ttactataga aataagtgta cgatttaaat
    17761 ttgtattatt tgtaataagt cttataatag gtcttaagat ggatgcaatg ataaaactga
    17821 ttacaaaagg cattaataat ggcaataaga attttaatac aatatataca atacttgtta
    17881 ttaaaactac atatgtaaat ttaattataa aattcttttg cttttgaata tccataatat
    17941 aaaacctcca atgtatttga tattattcta aatatggtct tttttacaga aaatatacta
    18001 ctgtatattg attataaact ataaagctta tgtaaagtaa ataaattatg taaaatttag
    18061 gttaattttg tataaagaga tacttttata ttttacaatt aataaccaaa tactaaaaaa
    18121 tattttaaat aaagaactta aaattgtgat aaaattaatt tatagatatt aaaaaaagta
    18181 taatatatgg tttttaagag gtgctaagat gaaaaatttt agagatttag gtgggaacaa
    18241 aactgaggat ggcagaactg taaagaaggg attattttat aggtctgcaa aattatcaaa
    18301 tctaagtgaa aatgatataa agattttaaa agagttaaat attaaatata tatttgatta
    18361 tagaagtgat gaagaagctc gtaaacatcc atcaacaata atatcaaata taaaaaatat
    18421 aagaatacca gcaatgagag agctagaaga atcaggagga agttttggtt ctattgaaga
    18481 tatgattgat ggattgtttg aaaaagatgg tgcatttaat atgttaaata atagttacta
    18541 taatttgcct ataaataatc cgtcatataa aaaattagta gaacttataa gagactattc
    18601 taatttacca atattaaatc attgtacagc tggtaaggat agaactggtg tggggagtgc
    18661 tataattctt atgattttag gggtatcgag agagaatata atgaaagatt atctaaaaag
    18721 taatgatttt gcagataaag agatagaaag atttatagaa tataagccta aatttaagga
    18781 cattccaaaa gaaaatttga aatatatatt tggtgtaaat gaagagtata tgaaaactgc
    18841 atttagaaga attgatgaag aatacattag tgtagaagca tatttatatg gagaatttaa
    18901 tttaaataaa gaagaaataa gaaaattaag aaatcaatat ctagagtaaa aggtgagttt
    18961 atgagtttaa atataacagc aagacagata aatataataa aaatattgtt aaattcaaaa
    19021 gatacaatta gtggtattgc cttaagtcaa gagataggat gttcttcaaa aactatacaa
    19081 aatgaaataa aggatattaa taaacaactt agaaatggta aaattttgtc aataagagga
    19141 gttggttata aactagaagg aagttttgat gaggtaaatc taaatagcaa tctctatgat
    19201 gatgtagata gagtagaata tataatcaga aaaatattaa ccttaagtaa tgaagaaaaa
    19261 aatactataa aacttgaaga tttagccgat tctatgtatg taagtgtttc aacagtaaaa
    19321 aatgatttaa aagaagtaaa aagaatgttg gaaaagtatt caatatcagt taaatctaag
    19381 cataaacaag gtatatgtgt tttagaaagt gaagataaga tattaaattg tatagttgac
    19441 ttaagcaata agagagataa tcaattgagt ttaaatgatt ttttaaatga tgatataaag
    19501 gaaagaaaac tattaataaa aaaattatta ttagacatat taaatgaaaa tggattagta
    19561 ttaactgata tagaatttaa gagtgtgtta aataatatat tagttatgtt aagcagacat
    19621 aagtataatg aaaaagaatt tatagagaat tatatagaaa attataggag caaaagaagc
    19681 tcaattttag aaaatgaaca aaacaaagaa attataataa attcaataaa aacattttgt
    19741 aaaaatttaa aattagctac atcaatagat ataagtaatg ataaggtatt tgaagagttt
    19801 ttatataagc atattagtag tttatgtaaa aaaataaagt taggtataaa tcaatcacct
    19861 ataatggctc aagatataaa aataaaatat ccatttgctt ttgaactagc taatatagct
    19921 aaaaagacaa tagaaaaaga tttgaagata gaaattaatg aagatgaagt tgcaaacata
    19981 gctctacata taggaggagc agttgaaaga gcatcttata ataataaaga taaggttttc
    20041 aaaactataa tagtttgtac ttcaggcata ggcacatcaa tgttaataaa agctaaatta
    20101 gagaatatat ttaaggaaaa attagaaata ctaaagatta ttccttcata cttagttgaa
    20161 tatataaaag ttatagatgt tgattttgta atatctactg ttcctattga agtaggagat
    20221 gtgccagtaa taaatatatc tccaatttta agtgagaaag aaattagatt aattgaaaaa
    20281 tatatagaaa caggaaatat atacctagat ttagatataa aaagcctgtt tgatagtgaa
    20341 ttgtttttta ctgatttggt gtttaaaagg aaagaagaag ttatagattt tatggcaagt
    20401 aaacttgtac aaaagggcta tatagatgag gatatgaaaa aatcttactt tgagagagaa
    20461 aaaatagcaa caacagaaat agggaatatg gtaactatac cacatggagc tattggaaaa
    20521 atttttgata ataaaatagc aattggtata ttaaagaagc ctatttcttg ggaaatatca
    20581 gatgttcgtt taataataat gttagccctt gataaagata aaatattaga ttatgaagta
    20641 atattttcaa aaatatataa aagagtagat tctatagcaa aagttataag tatatgtgaa
    20701 aatagtagtt ttgaaaaatt tgtaaattta tttaattaga tgggtagtat tgtatataca
    20761 cctgtaaatt tggataatat aaaactaaat aagataaaaa cgataaattt ctcttttaat
    20821 taagagaaat tttttgactt tcaaggatat taatataaag ttaagatata aacataaagc
    20881 aaataaataa aaaagaggtg gcaatatgat ttatacagtg acattaaatc catcaataga
    20941 ttatatagtt aagctagatg agctaaagac aggaagtaca aatcgtgtaa aagaagaata
    21001 cgtttatcca ggaggaaaag gtataaatgt atctcgcata cttaaggaac ttggaaatga
    21061 taatacatct ttaggtttta taagtggttt tactggagaa tatataataa gaacattgga
    21121 agaaaaagaa ttgaagacag attttataaa gataaaaagt ggtttttcaa gaataaatgt
    21181 aaaaataaag gcgttaaagg aaactgagat taatggacaa ggtccaaata tagatgatga
    21241 agacattgat actttatata aaaaacttga taagctaaat caagatgata ttttaatatt
    21301 ggcaggaagt ataccatcta ctttagatga aaaactatac gaaaatataa tggctagatt
    21361 agaaaaaaag aatataaaag ttgtagtaga tgcaactaaa aatcttcttc ttaatgtttt
    21421 aaaatacaaa ccttttctaa ttaagcctaa taacgacgaa ttagaagaac tatttggtgt
    21481 taaattaaat tcaattgaag acatggtaaa atatgctaga aggttaaagg aaatgggagc
    21541 tataaatgtc ttggtatcaa tgggaaaaga tggagcattg ttgattacag aggaagatga
    21601 agtgcttata agtgatgttc caaaaggaaa agtaaaaaat tctgtaggag caggtgattc
    21661 aatggtagca ggatttatat ctgggtattt gaatactggg aaatatgatt atgctcttaa
    21721 attgggagca gcttcaggta gtgcaactgc tttttcatat gacttagcca aaagagaata
    21781 cattgataaa ttggtaaatg aaatatctgt aaagaagttt taaaatatta aaaaatatat
    21841 agaaaggtaa ttaatattta tgaaaattat agatttatta gatgaaaagt caattaagtt
    21901 aaatttaaat tcaaaaacca aatcagaagc aatagaagaa ttagtagatt tggtcgcaaa
    21961 tagtggaaat ttaaatgata aagaaaacta caaaaaagca attttggctc gtgaagaaat
    22021 gagtacaaca ggaattggag aaggtgtagc tatacctcat gctaaaaatt cttcagttac
    22081 taaagcttgt atagcagcag cagtgtcaaa agaaggtata gattatgaat cgtttgacgg
    22141 aagtttatca aatctatttt ttatgatagc tgcaccagac ggagctaaca atactcatct
    22201 tgaagtatta tcaagattat ctacaatact tatggatgaa gactttagaa ataaacttat
    22261 aaattcaagc tctgaaaaag aatttaagga aataatagat aaaaaggaaa gagaaaaatt
    22321 cagtgaagaa tatcaagatg agaaagtggc tgaaaaaaat ataatagata ctaaagagaa
    22381 tgatgctaat aagtatccta aggttttagc agtaacagca tgtccaactg gaatagcaca
    22441 tacatttatg gcagctgaaa gtttaaataa aatggcagaa aacaaaggtg tatcaataaa
    22501 agtagaaact aatggttcag caggtgttaa aaataagctt acaaaagatg aaatagaaaa
    22561 tgcaacatgt ataatagtgg cagcagataa aaatgttgag atggctagat ttaatggcaa
    22621 aaaagtcata aaaactaagg tggctgatgg tatacacaaa gcggaagaac ttatagacaa
    22681 ggctgtaaat ggagatgctc caatttacca tggaggagat ggaagtcaca acgaatcaaa
    22741 tgaagaaagt gaaagtggat ttagaaaagt atacaaacac ttaatgaatg gtgtatctaa
    22801 tatgttacca tttgttattg gtggagggat acttatagcg atagcctttt tacttgatga
    22861 ttatactatt aacccttcaa actttggttc aaatacacct atagcagcat tttttaaagg
    22921 aataggagat aaagcttttg gatttatgtt acctgtactt gctggatata tagcatactc
    22981 tatatctgat agaccagcgt ttgttgtagg atttgtagga ggtgctctag caggtgatgg
    23041 tggttcaggc tttttaggtg cactacttgc aggttttata gcaggttatt tagtagaagg
    23101 gcttaagaaa atatttagtg tactaccagc ttcattggaa ggaataaaac cagtattgtt
    23161 atatccttta cttggaacac ttttgatggg aattataatg acatttttag taatacctcc
    23221 agttacagca attaataatg caatggttgg atttttaaat ggtttaggtg gaacttcaaa
    23281 aatagtttta ggattagtat taggtggaat gatggctgtg gatatgggtg gacctgtaaa
    23341 taaagcagca tatgtatttg gagttgcatc acttgaatca gggcagtttg aaataatggc
    23401 agcagtaatg gctggtggta tggttccgcc acttgctata gctcttgcaa ctacattttt
    23461 taagaataga tttactaaag aagaaagaga ttctggtaaa gttaattatg taatgggatt
    23521 atcatttgtt actgaaggag ctataccttt tgcagctgga gacccacttc atgttatacc
    23581 agcttgtgtt ggaggttcag cagtggcagg agcacttagt atgttgttca acgcagctct
    23641 tcgtgcacct catggaggag tatttgttat accagtggtt acccatccat ttgcatatat
    23701 attagctata gctgtaggtg ctttggttgg aatgatgctt ttagcattac ttaaaaaacc
    23761 attaaatcaa gaagtatagc aattaaagga tattcaagaa cttaaaatat gatgttttat
    23821 aaaaattaat atattgctga attattttaa aataaggatt ttatgaggct gttatataac
    23881 gatttgtaat tgttatcttg acagtctttt atttttatat aagtaaaata agtgtgtttt
    23941 gctttatttg tatttaaatt gtttttaagt tagaatataa agaaaattat atattatttt
    24001 aatattcact cgaactagaa aataatattt gttgtatttt ttgtatataa aaatatattg
    24061 taatattaaa aaaataatag taaactgatt gcataaaaat gatttttata taaattttaa
    24121 acagaagaat aaatttctaa cgaaattgga tatttcttct gtaaatacat attcaaaaaa
    24181 agaattataa gagtttagtc agcagtcgtg taagtaataa ggactttata aatatataga
    24241 aatataatga tatgagtata agtagaaaaa ttttatgatt tttacaatta ttttttataa
    24301 aaaagggagg ttatgtttgt tgaatttaca atcaaaatgg gtaaactttt taaagccaac
    24361 atatgtcagt gatgaaataa aaagaaaaaa actacaagtt ataatatttt tttctataac
    24421 ttatggttta tcttattcac taggtttatt agcaatatgt tttaatcatt taatagattc
    24481 agaaatttta gctcaattta tgatgatatt gccattatct tcagtttcta ttgcaaaatt
    24541 ttacacagag ggaagaaata atgataaata taatttctat tcatcagtaa tattattttt
    24601 tattatttac ttttttatat ttattattga atcattaaag ttaataagta ttaatcaatt
    24661 tcaattaatg tatcttatgt taattttaat aagtagttta tgtgttattg tttattctta
    24721 taaaataaca agtttatata tttttaaaaa ttttaagata ggtattttgc tattttttta
    24781 ctttttgttt tctcaaatta tttttggatt aataataagt gataatcaat ttgattataa
    24841 gaatttcttg aattatattc ttcttcctat aatatcatta gtatacattt atcctttttt
    24901 atgtgaagag tatggttgga gatctttttt gcaaagtatt ttatttgata gatttggcaa
    24961 aaaaataggg attgtaatgg taggcacttg ttggagttta tggcacttac ctttacaatt
    25021 tacactatat agtcctcaaa ctccaataat tggagctata gcccatttga tatatggtat
    25081 tggactttct atatttttag gttatgttta tatgaaaact aagaacatat ggttttgctc
    25141 tataatgcat gttctaatta atagcttagg attaattttt aatgattct
//
LOCUS       DAEMGG010000049        24576 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEMGG010000049 DAEMGG010000000
VERSION     DAEMGG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.049"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            74..1186
                     /locus_tag="KPK24_001891"
     CDS             74..1186
                     /locus_tag="KPK24_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG2299565.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(1550..2059)
                     /locus_tag="KPK24_001892"
     CDS             complement(1550..2059)
                     /locus_tag="KPK24_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HBG2299566.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVLVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(2061..2708)
                     /locus_tag="KPK24_001893"
     CDS             complement(2061..2708)
                     /locus_tag="KPK24_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299567.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(2722..3894)
                     /locus_tag="KPK24_001894"
     CDS             complement(2722..3894)
                     /locus_tag="KPK24_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2299568.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPAICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            4162..5538
                     /locus_tag="KPK24_001895"
     CDS             4162..5538
                     /locus_tag="KPK24_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299569.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILSNYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(5789..6148)
                     /locus_tag="KPK24_001896"
                     /pseudo
     CDS             complement(5789..6148)
                     /locus_tag="KPK24_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(6475..7005)
                     /locus_tag="KPK24_001897"
     CDS             complement(6475..7005)
                     /locus_tag="KPK24_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2299570.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(7093..8187)
                     /locus_tag="KPK24_001898"
     CDS             complement(7093..8187)
                     /locus_tag="KPK24_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG2299571.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGIISHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADRNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(8189..8635)
                     /locus_tag="KPK24_001899"
     CDS             complement(8189..8635)
                     /locus_tag="KPK24_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2299572.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            8941..10131
                     /locus_tag="KPK24_001900"
     CDS             8941..10131
                     /locus_tag="KPK24_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2299573.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            10301..11263
                     /locus_tag="KPK24_001901"
     CDS             10301..11263
                     /locus_tag="KPK24_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG2299574.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(11385..12047)
                     /gene="zmp1"
                     /locus_tag="KPK24_001902"
     CDS             complement(11385..12047)
                     /gene="zmp1"
                     /locus_tag="KPK24_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG2299575.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(12147..12232)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(12418..15336)
                     /locus_tag="KPK24_001903"
     CDS             complement(12418..15336)
                     /locus_tag="KPK24_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG2299576.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(15454..15539)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(15666..16844)
                     /locus_tag="KPK24_001904"
     CDS             complement(15666..16844)
                     /locus_tag="KPK24_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2299577.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDSIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            17219..17404
                     /locus_tag="KPK24_001905"
     CDS             17219..17404
                     /locus_tag="KPK24_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG2299578.1"
                     /translation="MFFISYIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(17469..20243)
                     /locus_tag="KPK24_001906"
     CDS             complement(17469..20243)
                     /locus_tag="KPK24_001906"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG2299579.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(20415..21671)
                     /locus_tag="KPK24_001907"
     CDS             complement(20415..21671)
                     /locus_tag="KPK24_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG2299580.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSTVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(21743..23245)
                     /locus_tag="KPK24_001908"
     CDS             complement(21743..23245)
                     /locus_tag="KPK24_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG2299581.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(23336..24526)
                     /locus_tag="KPK24_001909"
     CDS             complement(23336..24526)
                     /locus_tag="KPK24_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2299582.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         8009 a         4165 c         2949 g         9453 t
ORIGIN      
        1 aaagacgata ttactaatat aataattata atacttatag ttaatttact tttgttgaaa
       61 ggtggtaaat aacatgaaaa tttcagaagt cgctactcca aattttttgc tagatttaga
      121 ccagttagaa aagaatatag ataaaattca acaaatttgt acaaataata ataaacaact
      181 ttggcctatg cttaagacac ataaaagtac ttacatagcg agacttcaaa aaaatgcagg
      241 tgctagtggt tttctagttg gaacacttga tgaaggtgaa gctctgataa aagcaggttt
      301 tgaagatatt atgttggctt atccttacat gggagaagta aatataaata gaattgtgac
      361 tatggcatca aaaataaatt taatctgtgc tacagataat atagaatgtg ctaaggctta
      421 tagtgaagct ttttctaaat caaaaataga gtgcaagcta cttattattg tagattgtgg
      481 cttacaccgt tttggggttg aaccagagaa agtggttgaa cttgctactg aaatttctaa
      541 gctagaaaac ataaagattg ttggtataag ttcgcatcca ggtcaagtgt atggttgtag
      601 ttctccagaa ggtgttccag atgtttgtgt tcaagaagat tcttctatgg agaaagcata
      661 caaagctcta actgataatg gatttaatat agaaattgtc gcttctggta gtactccaac
      721 agttgaatct gaagctgctt ctaaaactat aactgctgta agacctggaa actatgtata
      781 ctatgatgct atacagatag gtttaggttg tgctactgaa gatatgtgtg ctcttactgt
      841 tgtgtgcact gtaatctcaa aaaatagcag tggatactat ttaattgatt gtggaagtaa
      901 gtgccttgga cttgataaag gtgcacatgg aaatagtagt attgttggtt atggtagggt
      961 atgtggacat ccagaactta ctattgattc actttcagaa gaagttggaa aaattaaagt
     1021 aaatggtgaa actgatataa aaattggtga tagaataaga attataccta atcattcttg
     1081 ttccactgca aacttgacaa gtaatttatt aggttttaga ggagatacaa tagaaaaagt
     1141 catagaagta gatattcgtg aaaattcaaa acaaatcatc ctttaaaaac agatttattc
     1201 tgatattaac atgaaagtat atttttctta aaattatttc taataactaa tgttaatgtt
     1261 ggataaataa aaaacataaa ttctagaaat ataaatttaa aagaagaggc tgtctcaaaa
     1321 tagagataag cctctctttc attgttgaaa ttatgaaaat atacttgatt tcatacaaaa
     1381 agagtatctc atgaactaaa aagttcactt tgagatactc tcttttttta attaatatac
     1441 acattaatta ctatcttagt ttattactat atatcaatta ctactcatta atccttgtat
     1501 atattaattc ttatatatca tttatagtta ggttataatt ttatgttatt tacattaatc
     1561 ccataacagt gagtaaaact cttactatta acatccccac tattgcatca aatataggaa
     1621 ctgccaacag taacattcta tacttagtac tggcatttgc tacaagcact attctggcat
     1681 aatgaccaac tagagtaccc attaacatac atggcataat taatatggtt gcttgtggtg
     1741 cagtgattag accttgtgca aataagtttg cagctgtagc tgctcctgct gccttagcaa
     1801 agaatgctgc tatcaaaact aaaacagatt ccccaggtag tccaaaaata cccattactg
     1861 gtccaaaaat atttcctaaa atatcaacta aaccagttaa ttgtaaaaac tgcactatta
     1921 cataacccat tatcatggct ggtaatatct gttcaacacc tatgtaaaaa cctttttttg
     1981 ctccccccat aaatatctca acagcagttt tcttaggttt actatttaca attgcatttc
     2041 cagtattttg ttgactcata ctacacagcc tccttagcct tattcttttt atttcttaca
     2101 cctatgatta atctaactag attagcacca agtattttac agaatatctc tataaggatt
     2161 attggaccta atgccaataa tgctattggt aaaagtggag cttgagtatt tattgtgttc
     2221 agtataacag ctgaaccagc atattgatat gctacaaata tagttctttc atcatctgtt
     2281 atttccccac tttcaaataa ttctttcgtc attattgagg ctacatctga actagtaaat
     2341 gaagatacaa atgctattcc tgctattcca ggtataccaa gcagaggtct aagcaatgga
     2401 ttaaatatct ttgatgccgc tttcattcct cccatagatt caactacatc tataagacct
     2461 acagcaaaac agacagttgg aatcaaagtc agagcaaata agaaaccatc ttttgctccc
     2521 gttcctcctt tacccataaa gtctacacca tctgcaatag taccaaatga acctgtcaga
     2581 ttagcaaaat ctaatgcttt aaggaagcct tgactatctt taaacactcc tgaaaataat
     2641 actatcaata caaataaaga taaataccct ttccatgtta ccttattatc tccagctgtt
     2701 acatccatat aaaatccccc tttatacctc aaatttatct aagtttaaaa taactgtaga
     2761 aattaatttc accctctcaa acatagaatc tactactgca tattctcttg atgtgtggtt
     2821 ccattcccct cttacaccaa atgaacagat tgcgggaacg tttgcaatag tcaaataact
     2881 agcatctgaa ctacctccaa gtctcttagc atttaattct ccaaatccat tttcttttga
     2941 cacttcattt acaaatgtat gaaatctcat aacatcttct gtagtttcaa atggcatcat
     3001 ttcacttata aaattaactt ctgtagaagt tccttctatg taagtttctt tacatatctc
     3061 tctaacttgt tctttaacat tctccatttc ttctactttt tcaaatctaa ggtctacttt
     3121 tatactacaa tcttctggaa tagaattttc cactcttcca cccttaatca cacttacact
     3181 tactgtagtt ccaacatcta aattagtcaa tttttgtatg cgtagtatct tattagccat
     3241 ttcttctata gcatttcttc caccttcaaa atcattacct gcgtgtgttt ctacaccttt
     3301 tacatgtata ttacatccta ttcttccttt tctacctaca cacaaactat tatctactaa
     3361 tccagtttcc atattaaaag cacaaagacc accttttgct tccctcaata taacatctgc
     3421 acctgttgaa tccttatgtc ctatctcttc atctccagaa aatactattt ttattggtct
     3481 ttctttgtat cctattttat ttaatgcttt aataacatat aaggaaatta ttataccacc
     3541 cttcatatct aaaacacctg ggccatatgc ttttccttct attattttaa atggattctc
     3601 tccaaatgta cctgtttcaa agacagtatc catgtgacct gaaaaaatta taggcttttt
     3661 atttatatta gaaccaagtg tacctatcaa tgtactacca ttatctccaa catctactaa
     3721 ctcgcaatct aaaccttctt tttcaaactc aaactttaat ctcttagcca atttatttac
     3781 gctttcttta caatgtgtat agctttctat attaacaatc tctttccata atgaaactat
     3841 ttcttctcta ttctcatcaa caaacttaga aatttcattt aaatatgtat tcataatctt
     3901 ctcctttatt ttcaaataaa atgtatttct ttcctaaaat ctaagtttat tttaggaaat
     3961 cttaaaagac tagtccttaa ttacaaaata gcacaattat tcttgacagt caacatattt
     4021 ttcagtcttt tttatatttt ctgatttttc tttaaatttt ccttttgtaa aataaattga
     4081 cgcgttttat ctactattat aaaatgaggt tataaattga ttgattagtt actatactct
     4141 cgaaatttta aaggaggttt tatgaaatat aaaattggtt ttttgacaac atcttatttt
     4201 atgtcagata agtttaaaca agctcttaaa gaattaaatg atacttgtga aattacattt
     4261 attgagctca aaggggagaa tccttttaat aatttaccta atatatacta tgagcaagta
     4321 gataattttg atggatttgt atgcagtggt atagttccat atagtgaact tataataaat
     4381 ataaaagaca taaaagttcc attaaatttt ttaaagcttg atgaaagaga tttttataaa
     4441 tacttattca aattattaaa tagccaaaaa gatattgact ttacaaaatc atttatggat
     4501 tttttaagag aggacaacaa ttattatgat atatattcat taatagactc taataaatgt
     4561 ccatatacaa ttaaagattt taatatccct caacctgtat atgactttaa aaaattaaat
     4621 gataaattat tggaaataca tttagactta ttgcgagata ataagattaa cctttctttt
     4681 actagaaatt acatcattag ttgtgaactt aataaacttg gatatgattg tatttacatg
     4741 gttccatcta cagaatcaat cctgaataca tttaaatctc tggtgaatga aataacatta
     4801 caaaacttag acaaaaataa atctgcaact tgtatagtta cagtaaatag ttcagagtac
     4861 ataaatgaag atttgatatt taaaaatgat gagatacaaa atcaaatata taacactata
     4921 ttaaattctc tatcaagaca tggattttat ggagttcagg tcaaaaaaaa tgatatgaaa
     4981 atagagatac acactactaa agaaatgtta aacaatatga ccaataacta tacagatttt
     5041 tttatttcag aagatttaaa agaaattaaa tatagcttaa atataggttg gggcattgga
     5101 aatacaaata ttcatgcaga gcaaaatgct cgtcaagcta atggtaaatc tgcttctttg
     5161 aatggaaact gtacttttat agtaaatgat actaatgata caataggccc tttatattca
     5221 aataaaaatt ctaataatat tgaagatagc tcaatagcca ataaagttgc ttcttttata
     5281 cctctatcta atacaaatgt atctaaaatc atgtgtatga ttaatgatag aaattcaaat
     5341 aatgtgtcag cagaaatatt atcaaactat atgaacataa ctcttagaag tgctaataga
     5401 atattgagta tcttatataa agcaggaatt gcaactattg taaatacaaa actcgataat
     5461 caaagaggaa ggcccaaaaa aatatataaa gttgatttct tatcttttct aaataagatg
     5521 cagaaattaa attcttaata tttaattttt taataagaac aatgtatttt ctctaatttt
     5581 aaagaatcaa aatgtttaaa ttttattaga atatacaact ttttacttta aaatacctaa
     5641 accttttatc agtgaaataa accataatat atcgtaactt attagaaaaa caaaataatt
     5701 tcatatacaa ataataaaat agccactaca atccataatt tgcagtggct actttttaca
     5761 ctatcactta ttacttaatt tttaaaaatt aattgtattt tttaaataat ttatacatac
     5821 ctattatcag taaagcaata aatacaagta tatctataaa aataattatt gtaacatgtc
     5881 tggcaatatt gtaaataaaa agtatagaga atatttctat agacaacgct acaaaacaca
     5941 taaaattcgt taaataatct gcaatagtgt cactataaaa agcatgtatt tgtaaccatc
     6001 tccatataaa tggacgtaaa aggattctga atacaacaca aatgacaggg taggcataaa
     6061 taaatttttt atctacataa ttagctacac cattattcca ttggatagga atttgaggtg
     6121 gcaactttgg aaatactgtt attgcaattt aaacactacc tctctattct attaatttat
     6181 aactataata tagtcttaaa tattttcact ctataaaatt gtatatttag tatatcttaa
     6241 aatcaaacta attatctttt ataagttttc atacaataat atctcctttc aataatatat
     6301 ctttttttat atttaaaact atcttatttt ctataaaata atgtataaac aaggtttaca
     6361 gtcaagtatt tttattattt ttttgatttc ataaaaaaag catagcaaat tatttgctat
     6421 acttttttac taaattattt attctatatc ttatatgaac ttattgttta actactacac
     6481 aatactagct ttaatcatca tattttttgc aaacatactg gcttcatata tttcttgttc
     6541 atctggatga ctttgagatt cctcatggtt atttaattgt tggcgaatat gttcgttgtc
     6601 aggagaaagt tttaacatct ctttatattt tgctttcaat ccttctgcta tcttaccttg
     6661 acatatgaat gaacctaata tttcattatc ttttgggata attgattcta tcttttctat
     6721 tatactagct ccataacttc cttttttact tgctcccatt gttccaaata gtgctatctt
     6781 tttattgtga atattttcaa gcacttgttt tgaattttta tcacaaattc ccttgtccac
     6841 ccaatagcct aagaatacta tatcataatt ttcatagtca atactataat caactaattc
     6901 atttacatct ttaatgtctt tttcttcttg aatttcatca tatatagtat aagcaatctt
     6961 tttagtattt ccagttaaac ttgaatataa aactaaactt ttcatgatgt ctcctcctct
     7021 ttatctaaat acttatctaa atcaccaaaa atatattaac cttaacaaat tatttatttt
     7081 tataaggata ttctaatcta gtatacatcc aaactgagag cgtgtttgta aagcatctgc
     7141 acacttctta ttacaataac agcacttttt aatctcatct actcttccat cttttacttt
     7201 ctgtaaccaa ttcctatctg ctagtaattg tcttgacata gaaaccatat ctactctatt
     7261 atttgaaata atactttcta taaaatctgg gctagacaat tttccaaccc cacatactgg
     7321 aagatttgtg tgtttcttta cttcatcagc taaatataaa aaacagcctt cttctttaaa
     7381 gtaaggatga ttattggttg gtatagtatc ttctaatttt gaatgatttg caagtgttac
     7441 atgaaaactg tttacaccta gggattcaaa ttttttaaca aaatactcaa cttccgaaag
     7501 taaaactcct gcatttccat aatgaggatt ttcttgtctt attgcaaatt tatagtctat
     7561 tggaatgtcc ttaacttcat ctcggatact actaattatt tcgcttgcaa atctacttct
     7621 attatcacac gttctaccat attcatcatt tctattatta aatatacttg atgaaaaact
     7681 tcctacaagc ctatcaccat gaatttgaat catgtcaaaa cctgcttttt ttgcattcaa
     7741 tgctgttact ttaaataaat ttataatatt tttaatttct tctttgggca tatttgttat
     7801 gtaatctttt actccgtcat tcattatttt ttttatctca tcatgtgaaa taattcccat
     7861 attcatagta tctttaatta aattaacatc ataatctgaa caaaatagtt gtgctgatac
     7921 ctttgcacca gaattgtgta tttcatctac aattttcttg tatttcatga ctccatcttc
     7981 atccgatagg cttatagcta ccttatgaaa actaggtcta atagatacat ctcctagagt
     8041 aatcagagct actcctgact tagcaatatc agaaaacttt ttaatttttt cttccaattt
     8101 aagacccatg cttgtaggtg caaaagatac tctatttttt aattccaaat ttccaatttt
     8161 aattggtttt aataaattac aatacatcct attcttcctc cacagctgtt tttatatcct
     8221 ttaccacatg taaattcttt accagttcct tatattctgt atccgagaac tcttttttta
     8281 tgtgattatc ccactttgaa aatatgtttt ttatatcccc taatacttcc tcacccttat
     8341 ctgttactct caactgaaaa gctctactat cattagggtt ttggattttt tctatgtatc
     8401 catcttgttc taacctagat atagttcttg ttgtatgagc tttatcaact tcaaattctg
     8461 tagaaacatc attcaaagaa caattcctat ttttattaat ataaattaaa tagaaatata
     8521 gaccattcgt aagtttatat ggttttaaac atttttcaca ataaagttta aagttttttt
     8581 gcaagttaca aataatatat gacaaacttt catactgtaa atttttaaaa tccatcttaa
     8641 atttcccttc tataattata atatgtttga ctatatcaac tatattatat taaacaatta
     8701 atatagtcaa gaatatttta ttctctaaat ctatgtttta ttaattcttt aaaaaactat
     8761 tatataaaat aatcatgata aaattagaaa ttggaatttt taatatgtac tgtacataaa
     8821 tatattttat ataaaagttt atattttcaa acaagtaatt tttcgaaaat tgtctaaatt
     8881 cagtaaaaat atggtaatat atacataagc aatataaatc tataaaaagg agtgttacat
     8941 atgatatcta ataaaatgca aactttagtt gcaaatagtt ctgttataag agctatgttt
     9001 gaagaaggaa agaaactttc tgatatttat ggtgaggaaa atgtttttga ttttagtata
     9061 ggaaatccaa gtgttgaacc tccagagact attaaagcag ttataaatga tattttaaat
     9121 gaagaatctc caaatttagt tcatggatat atgaacaact caggatatga ggatgtaaga
     9181 gatgctattg ctgaacacat aaataaaaaa gatggattaa atcttacaag agaaaattta
     9241 attatgactt gtggtgctgc tggtggtcta aatattattt taaaaactct tttaaatcct
     9301 ggtgatgaag ttattgcttt tgcaccgtat tttggtgagt ataaaaatta tacagaaaac
     9361 tatgatggaa aattaataga agttcctaca aatatagaga cttttgagcc agatttagat
     9421 gctctaaaaa atgctattac tccaaaaaca agagctttaa taataaacac tccaaataat
     9481 cctactggtg tcatatattc tgaagaatta ttaaaaaatc ttggagaatt attggattca
     9541 aaacaaaaag aatttaatac aagcatatac ctaatttctg atgagccata tagagaaata
     9601 atttatgatg gtgccaaagt tccttgtgta ctaaaatact atagaaattc atttatagga
     9661 tactcttata gtaaatcatt atctctacct ggtgaacgta taggatatat tgttgctaat
     9721 gggcaaatgg atgattttga tgatgttatg tcttcattaa atgttgcaaa tagaatactt
     9781 ggatttgtaa atgctccttc tctttttcaa agggttattg caagaagttt agatgcagaa
     9841 gttgatgtaa atatttataa aaagaatttt gaccttctat ataatagttt aatcgatatg
     9901 ggatattctt gtgttaaacc taatggtaca ttctatttgt tcccaaaagc tccaattgaa
     9961 gatgataaaa aattctgcaa tgatgcaaaa caatttaatt tattattagt accaggttct
    10021 tcttttggtt gtccaggtca ttttagagta tcatattgtg tatcttatga taaagttaaa
    10081 agttctttac cagcatttga aaaattagcc aaattatata atttaaaata actaaatcaa
    10141 agtaattcaa ataattaact accttagtat agaatactta cattctatat taaggtagtt
    10201 tataatttta tgtactatat tttatacata ccatattatt ccaatatgct tgaatattct
    10261 tatctatagt actaatataa attacaagga ggtgtttgac ttgaaaaaag gttatgttta
    10321 tataatactg actaccttat tttttagttc aatggaaatt agtctaaaaa ctgtcactaa
    10381 tgactttaat cccttacaaa taactttatc aagatttttt gttggcggtt tagtcttact
    10441 accttttgct gtaaaaagat taagagctct tagtctttct attacaaaat cagacttaaa
    10501 atactttgca tttttaggtt ttatgtgtgt agtagtaagt atgtcattgt atcaacttgc
    10561 tgttctaaac acaaaggcat ctgtagttgc tgttttgttc agttgtaatc cagtctttgt
    10621 aatgctttta gcttacttaa ttttgaagga aaaaatatat aggcacaatg taatttcact
    10681 tatattagaa gttcttggaa ttatagttat aataaatcca ttgcatacaa aactaactct
    10741 tagtggaata ttcttaacat tatcttctgc tattattttt tcaatgtata ctgtatttgg
    10801 taaaagaaaa accttaaaat ttggtggaat agttgtaacc tgctttagct ttatatttgg
    10861 tagtttggaa atgttgatat tggttttact cactaaaatt aattttatcg cagatatatt
    10921 aaataaaaat aagcttcatg tatttgcaaa tatccctatt tttagtggat ataccttaca
    10981 caatatgccc attatgattt atgtattcgt atttgttaca ggtgttggtt atgcgcttta
    11041 ctttatggca atggaagcta catctactag tcttacatct ttagtattct tctttaaacc
    11101 agtaatatct ccaatactag cattattaat acttaaagaa attatcccta taaacatggt
    11161 tataggaatt ttattgatag taattggttc aataatttct attataccta cgataattac
    11221 tcaaatgcat aataaacacg ttgaagcctt gtataaggag tagtaattga taatctatca
    11281 tagttataca taagacaaaa tttattagat ttaaacgcaa tataaaagag atgctattat
    11341 aatagcatct ctttatttat atttatttta aatttattaa aactctattt agctaaattt
    11401 tgcaaaaagc tataagtttg tggacaagct gattttaact tactatttgt atcttgattt
    11461 aaatagtaat atgcaaatga ttctgcaaaa aattcttctg gatatacacc taagtagttt
    11521 acattaccta aagaacgacc ttctttagca aatatttgtt tgaattgtgc tgatttagat
    11581 atatcattta atactatatg gtctattgca tgtgctgttt catgtaattc taaatttatt
    11641 gcatcatgcc ctttaccttt gttactaaat cctattctaa gagctactgt acttccacct
    11701 aaacctggaa catcatccca agtttttcca gtaccttccc atcccttagg aactacacct
    11761 tttaaataag catattcttt ttcatctgtt aactttccac ttaataattt tatttttaag
    11821 ttattttgct ttaatgcatt taaatacttt ccatctatat tagctaatct atttaccata
    11881 gcattagctt cgtttttatc gtaatttcca gttggaaaaa ctactatttg actaagtgtg
    11941 tctttatttt gttgtatagt agtactatct gcatgtgcac ttactggaac tgaacttatt
    12001 aagaatattg atattatagc tattaataat tttttacttg gtctcatatt attccctcct
    12061 ttgagttagg ccgggagtaa taaatttcaa aataaaaaag atacccaaga gtatctttgt
    12121 atatacaaaa agaaattttt actcttttcg gcaactggct ggcatggttt actaaacttt
    12181 agcccctata gctttgcgtg actaggtttc cctagttttg cctcatttaa ttttatatct
    12241 atattatagc ttatatcttt ttaattgtct atatcccttt ggaacttttt tctaaaaagt
    12301 tattattttt ttacataaat cttcaaaaaa aaatatcttc taacaatatt ttcattatta
    12361 aatattgttt tagaagatat tttttaatat aacttatatt ttttataatg ttagggtcta
    12421 atttgtattt ttatttcttc ttaatacgat aagtcctaca atagctccta taaccaaaag
    12481 tatttctcca ataattgtag tactatcccc tgtttttggt ggtactggtg gatttaccaa
    12541 agtatcatct ttaactttat tatttttttc ttctgacact gatggcttta ctacttctgg
    12601 tattggaata atctgtttaa caggagtttc tatcatctct tctgatggtg gtactggcgg
    12661 attcaatacc tctggtattg gcggatttat tatatcttct gatggtggta ttggtggatt
    12721 tataactata gtatcctctg tatcagatga acttgatggt ttccttggtt tatctgtgct
    12781 tggtggtact ggtggattta ctattggttc atctgtattt ggtggtgctg gtggatttac
    12841 tggtggattt tcttgtctta tgttttctat atttccaata tccactattc taccaatatc
    12901 tttattacca atttcaacct taattttatc cttcagtttt tcatatccac ttggagccat
    12961 tatctccact aaataatatg ttcctggttt ttttatacta gattgaatta taccatcttc
    13021 atcactaact aaatccttaa ctactatatt tccaaatgca tcttctaaat tgaacttagc
    13081 acctgaaagt acatttttat ctaaagcatc cactttttta gcttcaaaag cataaccttg
    13141 cttagtagtc tctactcctg gaggacttga accattaatc tgagccacat tttgtatagt
    13201 ctccccaact tttacatttg tgaagtctgc atcaaatgta acccaacttt gcacaggttc
    13261 cccttctttt atctgtttaa attcgtattt atttactaat cttattgtat tttttccatc
    13321 tccagcttct tttttaagtt caagattatc agaatatttt atattagata tttctagagc
    13381 tgagtttact tcatctaaaa gatttacttg tcctgcatca taaactccat aagttgacat
    13441 attgattttg tatttaacaa tctggttatc actactattt aaaacttttt tatcaacttc
    13501 tttttttgcg cttattaatc cagaattttt cttaaagttt atctcatcat ttttatcaat
    13561 ttcactatta tttgcatcaa gaacagctct atttatatag ctatcttcaa tactaattac
    13621 ctttaatgta taagctataa aaaactttga ttccacttct tctttactga gattaattgt
    13681 aaatccagtt ataactttat ctacttgccc actttcattt cttttttctt ctactttata
    13741 tgaaatatta ttggcaattt tttctttact ccaaggagtc cagctcccat tcattggaat
    13801 ttctacaact ctaaaatcat gcccagcttc tcctgaagta gttaatctca tgccttctgg
    13861 tatattatct gttataacaa cattacttat tggtgattta tcatcattta ttgaaatctg
    13921 atagtttatt acttgaccaa cttgagtaat atttgttgct cctgaatgtc ctttagtaag
    13981 aagataatca tctcctctat caaatatact tacagtagca tcatcttctt gtatcttctt
    14041 accatcagat tcaatcctag ctacattctt atatttatta ccatgttttg cgtttttgtc
    14101 actgattcta gccttatact taacagtata cgatttatct atatttccaa aattaactga
    14161 aatagaattt ttactctgtt caatattaaa atcactaact tcttttccat cacttgttac
    14221 tacacttaca gaaccattta tcaatgtcaa tccttcagga agctcatcat acaaaataat
    14281 atcttttaac tcttcctgct tttcatttac ttgtatactg taatttatat ctttattttt
    14341 aactggcata aatacagtag ttgatttttt tccttcaaca agctttttaa gtgttccttt
    14401 agcatctgga acatccgttt tatctccagg ttttatttct acatctatat ctccacttcc
    14461 actttctatt tttccagtag tttcaatatg tatttttgca ttagtatctt catcaacctt
    14521 tttaacatat gcttccaaac ctattttacc ttgcattaca ctttggccat taggtaattc
    14581 tctaaatgta agtgtatact ctccattact ttctgtttta tcaaagcatt cacttgtata
    14641 atctaaatta actttattga atacatctgg caccttaaaa gttattgaat ctcctgcttg
    14701 tattcctttt gatgttggag tataagttaa attaaatgat atttttttca ttgattccgt
    14761 ttctattgta ttgttattaa tttttacaac aaatccatag tcttcatttt cttcattaag
    14821 ttcttcttct aaaactttca ataaattttc atcttctaat agattatctt cctctggagt
    14881 tttttctgta tttttattgt ctagtggttt ttcatcttct aaagcttgtt ctgacttttt
    14941 attatctgtt gacttatctt cttctggtat ctgctctggc tttttattat ctattggctt
    15001 ttctccttct ggagtttgct ctggcttttt attatctgtt gacttatctt cttctggggt
    15061 ctgttctggt tttttattat ctgttggttt ttcttcctct ggggtctgtt ctggtttttt
    15121 attatctgtt gccttatctt tctctggagt ctgttctggc tttttattgt cgctttcctt
    15181 ctcctctcca gttgatgaat tagttgaacc actttgaatc tgactattct ctcctaattc
    15241 tgaagcataa gatacagttg gatatattgt agacaccacc atacttaaaa tcataattaa
    15301 actaattaat aatgcctttc tatttccttt cttcatgtaa atctcacccc tttaggttac
    15361 ctataattta taatctaatc ttccattaat atcgtatata aagtaatttt tactaaaaaa
    15421 ttatacaaaa aaagctatga aaataaataa atttatattc atagccggtt gcaccactga
    15481 ctcagtatat ccataaggtg cccaataata gaatatactt caacatctct ataatatttc
    15541 ataaaaacaa ttaaaattgc tttatttata ttaaaaatac aattatatac ttctattata
    15601 ttatgcctct atactattgt ctatcctttt tacatttttt accttatgat gagattaatt
    15661 aataattaaa taaaatcctt atctacaaca attacacaat tatattcagg atttatctcc
    15721 ttaactaatt ttgttatatc ttcttttaag tcatcttctg acatgttttt ggaaaattcg
    15781 gaaggattaa ctactatatc aaatattaaa ttcttcttgc ttccttttcc aactattcta
    15841 aaatcatgca tagattttac aagactattt ttcttaagta tttcttcaag ttcttttttg
    15901 gctgacataa cttctttatc ttcaagacat attgggtcca tgtgaattac tagatatata
    15961 tctaattttt cagatatttc tctctctgca gtatctataa tttcatgtat ttccattata
    16021 tctatgttgg atggaatttc tgcatgtata gatgcaatac atctaccaac tccatagtta
    16081 tgaataattg aatcatgtgt acctattata tatttataag ataacaatag ctctataata
    16141 gaatttacca tttcttcatc tggtgcttcc cctaaaagag gatttatagt atcttttaca
    16201 agagagaaac cagcataaag tatcactaat gatactacga taccaacata tccatctata
    16261 ggaaaattag taaatctagc tacaataaat gatataacta cacagcttga agtaaataca
    16321 tctcctaaag catcaagtga aactgctttt aaggctgaag aatcaatttt atttcccata
    16381 aatttattaa aacgactcaa ccagatttta atcattatag atgcaattaa aagtataagt
    16441 ggtattactt caaattttac aggaattgga tttactatcc tttctataga tgactttaca
    16501 aattgaagtc caaccaacat caccataaat gctactatta atgctgataa atactctatt
    16561 cttccatgac caaaagggtg ttctttatca gctggcttac ttgctaattt aaaacctatc
    16621 atagttattg ctgaagatgc catatcagaa agattattaa atgcatcagc cattatagct
    16681 atactagatg tgagcattcc tatgaatacc tttatcacaa ataaaagtaa attggataat
    16741 atacctacta caccagctac ataaccatac ttatttctaa catctgtatt ttgaacgttt
    16801 tcgctatctc tgataaatgt tttgactagg attttggaaa acatgaaact tacctccctg
    16861 ccctttattt tattaacaaa ttttataatc tatatcttta tttatgataa cataatttat
    16921 atataaaatt cttaaaaatc actctacttc agtaaaatat tattctatat atctatttag
    16981 atacgaattt tatatttagt aaagttttta tggcttagtg tctgtttaat agattctata
    17041 taagttttta caaagattat ttattatgat aatatcattt ttcccaagta ttacttagaa
    17101 tatgattttt ttgttttgtc tgtacatatt ttaaatttat actatattta tctatatttt
    17161 ataaccttat gatacactat aactataagt ttttaaatgg aaaggaatga aataaatcat
    17221 gttttttatc agttatattt acacaaaatc acctattaaa tttgatactc ctttgcaaaa
    17281 agaggcatat aaaatattac aaaaattaga tattgacttt gaatgtgttg acacagatga
    17341 agcaattaca atggaagatt gtgttcagat taataaaaaa ttaaatatga aaatggtaat
    17401 ataaaacaac attaaacaaa caaaaaccgc aaagttacaa tatatagtac tttacggttt
    17461 cataagaatt aatttttact attaaagttc atagttctac tatttttcat tgttctaaga
    17521 attagcttta caaattcatt tgacaatagg atagtggatg caaatagtat tatctttatc
    17581 cacatatcaa agtccaaaga aacagaatta aagaaacttt ggaacacttg tgtaaatatt
    17641 atttgaatta tccctgtaac tactattatt tgcaatgcca gtttattttt aaagaaattt
    17701 ggtattgtac tatttaaacc aaactctcta caatttaatg cattaaaaag ggcactgaat
    17761 gcaaataaag aaaatataac agtaccttgt tcttctgatg tagctcccaa tatattaaaa
    17821 gctgattgag taaatacaat tgtaattata agaattgcat ttataataat atttacaaac
    17881 atagacctag ctattattcc agaatgtcta tttattggtt ttcttttcaa tacataatct
    17941 ctaacaggtt ctaatcctaa tgctagggct ggaggaccat ccattattat atttacccat
    18001 aataattgta ttgtagtaaa tggcatatcc ttaccagtaa gttgagatat tatagcaatt
    18061 ataaatgcta ctatatttac agtaagctga aactgtataa atctttgaaa gttctcatat
    18121 atacctcttc cccattttat tccctctact atagtactaa agctatcatc agtcaatatt
    18181 atatctgctg catttttaga tacttctgtt cctgaaatac ccattgcaat tccaacatct
    18241 gcttgactaa gtgctggggc atcatttata ccatcacctg ttacagctac aacttcgcca
    18301 cttttttgaa gtgctgaaac tattctcatt tttgtatctg gtttacttct tgcaacaata
    18361 gatattcctt tgatttcttc tctcaattct ttatctgtca atacatctat atatgttgct
    18421 tcaactgcct ttttcccatc atttaataat cctaattcat ttccaatagc tgttgctgta
    18481 tttatattat ctcctgtaag catttttaca tctactcctg cattaaatgc tttatcaatg
    18541 gaatctttga ccccttctct caatgggtcc actattccta caaatccact aaaaactaaa
    18601 ttatcttcct tcatataact tcttgaattt cctattaaat tcaattcacc ttctgaagtc
    18661 attgcaactt ctgttttaga attactcatt tttttataag caaatcctaa tgttctcata
    18721 gatttaattt gtaacttctt aatctcatct aaaatacttt tttctacttt tggagttatt
    18781 ggaacaatat tttttccttg ttgtacataa gaacattttt ttaataatac ttctggagca
    18841 ccttttgaaa gtaatatatc tgaatcctct tgacgaatta atgttgacat cttcttttct
    18901 tctgaactaa atggtatttg tgatatcaaa tacgtttgtt ttctcatttc attatagttc
    18961 ttatcattat gatataaaag taatgcacac tctgttgcgc ttcctatata tttaaatgaa
    19021 ttatcttctt tttctatatc tgcagttgaa tttactatac agttttgttc aaaatagcta
    19081 ttactttgat actctcctcc acttatatat ttaccatcaa catatgctac ctctaccatc
    19141 attttgtttt gtgttaaagt ccctgttttg tcggaacata ttacagaaac agaacctata
    19201 gtttcacagg cttctttctt tgttactaat gcatttatct ttgccatctt ttgcattgtt
    19261 atagctaagg taatatttat catagttggc aaaccttctg gaacagttgc aactattaat
    19321 gcaatacaaa ccatatatgc atttttagca ggttctaatg attgtaagaa agataatata
    19381 ctgcttgtat ctaaaattaa catacctttt ctctgcatct gtataatcat aaacatacac
    19441 aataaagtag ctattatgcc tgatattcct gctattttag cacccaactt ccctaattta
    19501 atttgaagtg gagtttgctg gtcttcattt gataaattct gtgctatctt acccatctct
    19561 gtcttatcac cagtatgagt gacaaccatt atgcccctac catatgctac aagagtccct
    19621 ccaaatacca tattgacttg ctttgcaggt attgcatcct gttcgattat ttctgtttta
    19681 gaataaacta cttccatatc taatacagca tcagcattct tagctacatc atcagattct
    19741 cctgttagca tatcttctct aagtttcaaa tttatagact gtataagtct tccatctgct
    19801 ggtatcatat ccccagtttc tatatataca atatctcctg gtactaaatc attttttgat
    19861 attttaatta tcttcccatt tctaagcacc tttacttcta tattttctgt taattttgac
    19921 agggcatgag ctgctttttt agattttcct tcagttatta tgcctataga aattccaata
    19981 aaaatagccc ctaaaatacc taatgcgtca tgtaattctc ccacaaaaga acttattact
    20041 gctgctccta tgagaattaa tatcataggt tctgtcaaac tttctcccaa ctcgtcccaa
    20101 aatgttctac cttcttttat agtaaattca tttaatccat atctaagctt cctttcttct
    20161 acctcattat catcaagtcc aatttctgga tttgttttta agtattttaa tacttctttg
    20221 gtaggtttat tataatacct cataacttta tcccccttgt taattatcta taaggaatta
    20281 tatgcttatg atagcttgta tattactttt caaaaaaaat aaggtgttga ttaatcaaca
    20341 ccttatagtg gttatatatt attaaatttt atgttacagg ctttataaat tgtgagctat
    20401 acaaatcaaa atatttaatc ttcaaaatac ccttctggat ataatggaaa cttagatata
    20461 agttcttgtg cctctgcttt acattcatct aatacttctt tattatcaat attttctgct
    20521 accttattca ttatccctgc tatctgtata acttcttttt ctttcagacc tctttgagat
    20581 attgatgtaa gacctatacg aacaccacta gttacaaatg gtgattgtgg gtcgtcaggt
    20641 ataacatttt tattaactgt tattcctaca tattcaagag ctttttcaaa ttgttttcct
    20701 gtaagattct tacttcttaa gtcaaccatt actatatgat tatcagtacc tccacttaca
    20761 atactaaaac catgtgaaat cagttcagaa gctagagtct gagcatttac tacaacttgc
    20821 tccattatct ttttaaattt atctgtactt aaatataata gtgaaaaggc ttttgctgca
    20881 actgtattaa gatgtataga acctaatgtt cctggaaaaa cacctttatc gagtttcttt
    20941 gcatgttctg ctttacaaag tacaatacca cttcttggtc cacagaaagt ctttgtcgta
    21001 gatgaagaaa caaaatctgc ataaggtaca ggactaggta ttactttcgc tgcgactaat
    21061 cctgcaacat gtgccatatc taccataaag taagctccta cttcttttgc aacctttgat
    21121 attctttcat aatctattaa tcttggatat gaacttgccc ctgcaattat aagttttggt
    21181 ctacattctt ttgcctttgc ttcaagagca tcataatcta tacgtccagt atttgggtct
    21241 accccataaa attcatattt ataaatgtca tgtaaaaaat taactgtgct accatgtgtc
    21301 aagtgtccac cttggtcaag acgcatactt aaaacagtgt catttggttt taatattgat
    21361 gaatatacac aatagttagc tgtagaacct gaatacggtt gtacgtttac atgttctgct
    21421 ccaaatactt cttttgcacg ttttattgct agggtttcaa gtttatctgc ttcttcagaa
    21481 cctgcttgaa aacgagcccc tggatagcct tcttctgttt tatttgtaaa tacacatcca
    21541 cttaattcaa gtacttctgt tggtgctgta ctttcagaag ctatcatctc aatattgtgt
    21601 tcttgtctaa ccaactcatc agctacaatc ttgtataact ctgggtctga aatttttgtt
    21661 gtttttaaca taataatcct cctcttaatt aaagaatata ttttattagt ttaatcttca
    21721 ttgataaatt tattataacg gtctataaaa cgtaagatat tcctccacca ggtcctaatg
    21781 gtatgccaat acccatgaat agtaacaata ttattatttc tattaatgta aataccactg
    21841 agtatggaat catattcgat atgacagtac ctatacctat atctttatca tatcttctgg
    21901 caaaggccaa aataactgga aaatacggaa aaagtggact tattggattt gttgaagcat
    21961 ctccaatacg ataagctatt tgagttaatg ctggattaaa gtctagtagc ataaacatgg
    22021 gaacaaatat aggtgctaaa atcgcccatt ttgcagaagc acttcctaca aataaattga
    22081 taaatccaga tagcaatata aatcctatta tcaatccata gctaggtatc cccgactctg
    22141 ctagtaattt tgctcctgtt attgataaaa ttgttcctaa gttactaaga ttaaataaat
    22201 taataaactg ggatgctata aatgctagga ctatatatcc acccatatca ctcatactac
    22261 tacccatcat agatactaaa tctttgtcac tttttatctt cttacttact tttccataca
    22321 ctaatcctgg agttaaaaat ataatagtga ttataggaac tatacctttc attaaaggtg
    22381 cattactaga tagtatagaa ccagtctctg gatctttcat aaatgcattg tcacctatac
    22441 ataaggcaac tatcactact actacaacta acaatgataa taaagcgtat tttataccct
    22501 tattttcaag ttcactcaat tcctgtatat ccttatcatt tgttgaatca ttatattttg
    22561 taaaacgagg agctataaat ttttcagtta caaatgctcc tgcaaaagtt aaaactatcg
    22621 ctgatataaa agcaaaataa agattcattg ctggattgcc ttggtaagat gggtccatca
    22681 tttgagctgc tggtattgta aatccagcta acaatacatc tgtagtacta agcataacat
    22741 ttgctgcaaa tccccctgca acacctgcaa aagctgcaaa catacctaca agaggatgtc
    22801 ttccaattcc taaaaacaca agtgctgcaa gtggtggtaa tattataaat cctacatctg
    22861 ctgctgtatg tgataacata cctgcaaata cgattgctac agttacaagt ttttgtggaa
    22921 ctttacttat accctttttc attaacactt ccataaatcc agatttctca gcaacacctg
    22981 ctccaagcat tgtaaccagt actaatccta aaggagcaaa tgactgaaag tttgtaacta
    23041 tgctacctaa atatgattga atctgctctt tctctagtag atttactacc tttacaactt
    23101 cttttgtaga aggatgtatt tcttctactc ctaaattaga tataactgct gacaatatta
    23161 caacaattat acatagtaat aaaaatatgg ttactgggtc tggaagttta tttcctgctc
    23221 tctctacaac atttagtgcc ctcatcaata atccactatc tttgtttttt tcctgtgaat
    23281 taattttcct caaactaata ccccctcata aactttatct ccagaatgct ctatcttaaa
    23341 ttttattttt taatttatcc atataattta taacagactg acatagtaca tttgtaccta
    23401 ctaatataga atttttatca gctttaaatt taggattatg ccactgatat atacaatctt
    23461 gttctttgtt gcctacccct aaccagtaaa aaaatcctgg ggctttttcc atgaatatag
    23521 aaaaatcttc ccctcctgta gaaggtattg gatcaactat agcatcttct gaatacaaat
    23581 cacacacaga cttacaagct attgcgtaca tctctttact atttataaca gctggtaaat
    23641 caaataaata atctagcttt gcagttgctt gatatccacg agctatctca caaactatat
    23701 tttctaaatt ttctgatatt ttctttcgta cttcattact aaagcttcta catgtgcctg
    23761 tcatatctac cttttctgaa attacattat ttgcagtccc tccatttata gtacctattg
    23821 atataacagc tgattctaat ggacttatgt ttctgcttac tatggtttga atacccatta
    23881 taattgcact tgatgctact atagggtcta ttgttctgtt gggtatacct ccgtgacctc
    23941 caaatccatt tacttctatc ttaatagtgt ctactgctgc cattaaccca cctaacttaa
    24001 cacctatttt tccacaaggt atatctggat gattatgaag tccaaatata gcatcaactt
    24061 ttggattttc cattactcct cgttccacta acatcttcgc acctttattg acttcttcgg
    24121 ctggttgaaa tataaacttt acatttccat gaagactatc tctaatatga gacaatacat
    24181 atgcacatcc tacaaggcaa gatgtgtgta tatcatgccc acatgcatgc attataccat
    24241 catatctgga tttatactta atatcaacct cttcatatat tggcaatgca tcaatatctc
    24301 ctcttagtgc tactgtggga ccatcatact tcccttttaa taatccaaca actcctgttt
    24361 ccattcctaa atctaatatt tctatgtcta aactcactaa taaatctttt atagcttttg
    24421 tagtcctaaa ttctttaaaa cttaattcgg ggtgcatatg aatatctgta aaaaaatcta
    24481 aaatatcttc tttatacttt tctgagatta aaataagttc atgcatacta acacctctcc
    24541 aaaattttat ttttttattt tttataattt aactac
//
LOCUS       DAEMGG010000050        24540 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEMGG010000050 DAEMGG010000000
VERSION     DAEMGG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.050"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            112..228
                     /gene="rrf"
                     /locus_tag="KPK24_001910"
     rRNA            112..228
                     /gene="rrf"
                     /locus_tag="KPK24_001910"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            347..538
                     /locus_tag="KPK24_001911"
     CDS             347..538
                     /locus_tag="KPK24_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG2299583.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            693..2102
                     /locus_tag="KPK24_001912"
     CDS             693..2102
                     /locus_tag="KPK24_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2299584.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILENLYKM
                     DTTEILGTDNLHFPEGIIKNSQEKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEVSEETNVLKGISISNVIDIVDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPSFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRTFKNNVDSNINIYDTNDKTKIFISSKNMGLTGYELESILRNKYNIQV
                     ELSNYYGVLLICTIGNIAKDFSSLETALNNVIKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            2223..2912
                     /locus_tag="KPK24_001913"
     CDS             2223..2912
                     /locus_tag="KPK24_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBG2299585.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSEKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKSMER"
     gene            2915..3850
                     /locus_tag="KPK24_001914"
     CDS             2915..3850
                     /locus_tag="KPK24_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG2299586.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3847..4740
                     /locus_tag="KPK24_001915"
     CDS             3847..4740
                     /locus_tag="KPK24_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG2299587.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4818..5564
                     /locus_tag="KPK24_001916"
     CDS             4818..5564
                     /locus_tag="KPK24_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG2299588.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSVKLNGLEERVKIINED
                     IKYIDKVLDVNGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5565..6398
                     /gene="rsmI"
                     /locus_tag="KPK24_001917"
     CDS             5565..6398
                     /gene="rsmI"
                     /locus_tag="KPK24_001917"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG2299589.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHKFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRKISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6421..7125
                     /locus_tag="KPK24_001918"
     CDS             6421..7125
                     /locus_tag="KPK24_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBG2299590.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYTEKGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7313..7741)
                     /locus_tag="KPK24_001919"
     CDS             complement(7313..7741)
                     /locus_tag="KPK24_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG2299591.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7778..9208)
                     /locus_tag="KPK24_001920"
     CDS             complement(7778..9208)
                     /locus_tag="KPK24_001920"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG2299592.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9340..9924
                     /locus_tag="KPK24_001921"
     CDS             9340..9924
                     /locus_tag="KPK24_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG2299593.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9940..10461
                     /locus_tag="KPK24_001922"
     CDS             9940..10461
                     /locus_tag="KPK24_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBG2299594.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10527..10773
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10857..12794
                     /gene="metG"
                     /locus_tag="KPK24_001923"
     CDS             10857..12794
                     /gene="metG"
                     /locus_tag="KPK24_001923"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG2299595.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     EIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFRKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12824..13594
                     /locus_tag="KPK24_001924"
     CDS             12824..13594
                     /locus_tag="KPK24_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG2299596.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFDIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13936..14367
                     /gene="phnG"
                     /locus_tag="KPK24_001925"
     CDS             13936..14367
                     /gene="phnG"
                     /locus_tag="KPK24_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG2299597.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14382..14957
                     /gene="phnH"
                     /locus_tag="KPK24_001926"
     CDS             14382..14957
                     /gene="phnH"
                     /locus_tag="KPK24_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG2299598.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQEDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGVREEFIERLSTINK
                     DYPIGIDLILVDKKGELAFIPRSSKLSWEVL"
     gene            14960..16045
                     /locus_tag="KPK24_001927"
     CDS             14960..16045
                     /locus_tag="KPK24_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG2299599.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYIRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVDLLEEDEKKNRDFKDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKSEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSENLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            16065..16934
                     /locus_tag="KPK24_001928"
     CDS             16065..16934
                     /locus_tag="KPK24_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG2299600.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAVSIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRNFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16927..17793
                     /locus_tag="KPK24_001929"
     CDS             16927..17793
                     /locus_tag="KPK24_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299601.1"
                     /translation="MSKINDSKRLESIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILKLSDQKKRYIKNHFMGIVYQNPHLGLNLDFSNGGNIAEKLLMANLYNVEKIR
                     DRAKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17834..18547
                     /locus_tag="KPK24_001930"
     CDS             17834..18547
                     /locus_tag="KPK24_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2299602.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACNNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18548..19717
                     /locus_tag="KPK24_001931"
     CDS             18548..19717
                     /locus_tag="KPK24_001931"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG2299603.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYIIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLDKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYREYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKLDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19721..20557
                     /locus_tag="KPK24_001932"
     CDS             19721..20557
                     /locus_tag="KPK24_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG2299604.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGVTIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLFYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20558..21553
                     /locus_tag="KPK24_001933"
     CDS             20558..21553
                     /locus_tag="KPK24_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2299605.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21571..23259
                     /locus_tag="KPK24_001934"
     CDS             21571..23259
                     /locus_tag="KPK24_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG2299606.1"
                     /translation="MITSKNKRRINDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYIENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     NFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVVGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYFLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSISIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23271..24338
                     /locus_tag="KPK24_001935"
     CDS             23271..24338
                     /locus_tag="KPK24_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2299607.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCGTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9770 a         2757 c         4394 g         7619 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaagaaact agtgttacta
       61 gattctaaaa aattattaaa aacttaatag aaatattaag cataaagatt atgtggttac
      121 aatagcagag aggatacacc tgttcccatt ccgaacacag aagttaagct ctctagcgct
      181 gatggtactt ggtgggaaac tgcctgggag agtaggacgt agccacgtaa tttttttatt
      241 ttttgtcaaa aaaaacaaaa aatacgtatt tcttttttca tttcgggaaa actaaacaca
      301 ggaattatgg actttataaa aatttaattc ttgaggagtg atataagtga aaaaagttgc
      361 tttaatttta gctataatag gtgcacttaa ctggggtgca attggtattc taggtacaga
      421 cttaataggt agtatctttg gtggaacata tgaaatggta agtagaataa tatacttcat
      481 tgtaggttta gctggattat acttaatacc aagtttaatg tctgatgata gagaataatt
      541 tatgctttta tgttaaatac ctcattctaa atgtttagaa tgaggtattt aattttataa
      601 aaatattaat ttgtttcttt tttaatggta aaatagataa gaattgaagt tttaaaaaga
      661 agtattatgt aaaaaaggag tactattaat atatgtttat tattaatgaa ttaaataaga
      721 ttgtaaatga agaagtaata tcttttcata tgcctggtca taaaaaggga caggtttata
      781 aaaagttagg atataaagat atactagaaa atttgtacaa aatggataca actgagatat
      841 taggaacaga taatttacac tttccagaag gcattataaa aaattcacaa gaaaaagcat
      901 caagagtatt taaaagtgat tatacttgct atttagtaaa tggtagtagc tgtggaatac
      961 aatctgcaat aatgtcagta tgtagcccta aaagtaaaat aataacaaat agagattgtc
     1021 atcagtctgt tataaatgga tgtattttag gagatataga tatagagtat gtgccttgtg
     1081 aggtaagtga agagacaaat gtcttaaaag ggatatctat aagtaatgtg attgatatag
     1141 tagataaaaa cttggatgca aaagcagtac tgcttactta tcctacatat tatggaatga
     1201 catatgattt agaatacata tgtaattatg cacatagcaa aaagatggta gtgatagtag
     1261 atgaagcaca tggtgcgcat ttagggctta gtgagaggct tcctaaaaca gctcttgaac
     1321 aaggtgcaga tatagttgta cagagtacac ataaaactct tccatcattt acacaatcgt
     1381 caatgataca tatacaggga gaccgagttg atatagataa aatattgaac atgttaagaa
     1441 ttacagaatc ttcaagccct tcatatttat tattatcttc tctggaatta gcagtagata
     1501 tatatgaaaa taaaggaaag gaattaatgg aagagctttt aaataatatt agaactttca
     1561 aaaataatgt tgattcaaat attaatatat atgacacaaa tgataaaaca aaaatattta
     1621 tatcatctaa aaatatgggt ttaacaggtt atgaactaga aagtatttta agaaataaat
     1681 ataacataca agtggaatta tctaattatt atggagttct tttaatatgt acaataggaa
     1741 atattgctaa ggatttctct agtttagaaa cagcattaaa caatgttata aaggaagact
     1801 ttaaatttga aaagttagaa aataaaaaat accctgttgt tattccaaag aaaatattaa
     1861 cacctagaga agcattttat aaaactaaaa aaagtgttaa aatatatgac agcataggta
     1921 aaatttgtgg tgaatgtata gtgccatacc cacctggaat aagtgtaata tctcctggtg
     1981 aagtaataag taaagaaata atagactatc taatgttttg tcattctaaa ggtatgattg
     2041 tgagtggatt gaaagatgta gatttaaatt ttatacaagt aatagattta gaagacattt
     2101 aaaaattatg atataaaagt agatagtttg aaattgtgaa gagaaataaa tagactgctt
     2161 atatagagct atctaataaa agtactaaaa aagtaaaaag tgtttgtatg gaggatatga
     2221 aaatgaaagg taagctaata attatagaga gtggttcaga tgcaagtgga aaggctactc
     2281 agacagaaat attatatgaa agattaaaaa aagaaggcta tatgattaaa aaggtagaat
     2341 atccaaatta tgaatctcca tcatctgcct tggtgaaaat gtacctaaaa ggagactttg
     2401 gtaaaaatgc atcagatgta gacccatata tagcatcaac tttttttaca gctgataggt
     2461 atgcatcatt taaaaccgat tgggaggagt tttacaatga aggtggaata attatcgcag
     2521 atagatatac aacctctaat atggtacatc aggcctctaa aatggatatt tgtgatagag
     2581 aagagtattt aaattggtta gctgattatg aatttaattt atttaagata ccacaacctg
     2641 attgtgttat atttttagat gttcctattg aatttagtga aaagcttatg gagaatagaa
     2701 aaaataaaat tacaggggaa gaaaaaaaag atattcatga aagtgatata gagtatttag
     2761 agaaatctta taacaatgct ttatatatag cagataaata tgattggaaa aaaataaatt
     2821 gtgttttaga taacaaatta agaagtattg aaagtataca taatgaaatt tataaaatag
     2881 ttcttgattc aataaaatct atggagagat aaatatgtat tttgaaaata ttatagggca
     2941 agattttgca aagaaatatt taacaaattc tataaagaaa aataaactta ataatgcgta
     3001 tatgtttgaa ggaatggatg gaataggaaa aaagaaattt gcagatgaat tatcaaaact
     3061 attacttgat tatgaaaatc ttgaaaatag tccagactat gtactcataa aaccagatgg
     3121 aaatagcata aaaatagctc aaatcagaaa tttacaatca gatatagtaa taagacctca
     3181 taaagactat aaaatatata taatcaataa tgctgaaaaa atgactgtgg aagcccaaaa
     3241 tgcactttta aagacgctag aggaacctcc aaactatgct ataataatct tagtcacaaa
     3301 caacaaggaa tctttattag aaactataaa atctagatgt gatataataa agttctcacc
     3361 tataccaatt gaggacttaa aacgttattt gataaataca ggtatagaag aagaaagagc
     3421 tcaattattg gctatttttt cgagaggaag tattgagaat gctctaaatc tatctcagtc
     3481 ttctgagttt tcaatgatga gagaagatat acaacaatat attcagataa tgttagataa
     3541 aaacatagta gagattttaa atataccaaa taatatggaa aaatatagag gtaagattat
     3601 agctttatta gatatgatga taaactattt tcgggatatc atcctattaa aagaaaatgt
     3661 aaacaagaac atgttaataa atgtagataa gctagttttc attcaaaata tgagtggaaa
     3721 aattagttat tctcaattgt ccaagattat tgatataata gaagatgcaa agagcaaaat
     3781 aaaaagcaat tgcaatttca atataagcat acaagttatg tctttaaata tatacgaggt
     3841 gattaaatga taaagatagt aggtataagg tttaaaagtg ctgggaaaat atattatttt
     3901 gaccctgttg attttaatat agagcaagat atggatgtag ttgtagaaac tgcaagaggc
     3961 ttggagtatg ggaaagtagt cgttggtcca aaagatatgg atgaaagcga attagtttct
     4021 cctttgaagc caattataag aatagctaca gaagaagata aaaaagtata tctagaaaat
     4081 aaggaaaagg caaaagaaac ttttgagtta tgccaacaaa aaataaaaga acatgagtta
     4141 acaatgtttt taatagattg tgagtatacg tttgatagga ataagttgat tttttacttt
     4201 acagcagaag gaagaataga ttttagagaa ttggtaaaag acttagctgc tatatttaaa
     4261 actagaatag aacttagaca aataggtgtc agagatgaag ctaaatctat aggaggtcta
     4321 gggccttgtg gtagaaaact ttgttgttca tcttggttag gtgattttca accagtttca
     4381 ataaaaatgg caaaagacca aagtctatct ctaaatccaa caaaaatatc tggaatatgt
     4441 gggagattat tctgttgttt aaaatatgag catgatgtat atagtgaggc aatagaaaaa
     4501 atgcctgttg taggtgcatt ggtgcaatca gatgatggaa aaggaaaagt tgtagaagta
     4561 aatcctttat tagaacaaat taaagtggaa tttcaagaca agacagtaaa gatatacaca
     4621 agagaagata taaaaatact tagagagcct aaaaaatgtg atggctgtgg tggtaaatgt
     4681 aaggataatg atggattaga tgaagccaca ctaagagagt taaagaaatt agaagattaa
     4741 atttaaggta gggcttttcc tacctttttt aaagcaatta atgcttaaag ttattaatat
     4801 ttgaggagaa tttatatatg caattaaaag attcagaaag aatagatgat ttacaattaa
     4861 aaggtcttaa aattatacaa gatactaatg gtttttgttt tggtatagac gcagttttat
     4921 tagctaattt tactaaaata aaaaaagatg ctaaagtagt agatttgggt actggaactg
     4981 gcattatacc tattttgata gctggaaaaa gtgaagctaa aaaaattatt ggtgtagaaa
     5041 tacaagaaga tgtatatgaa atggcaactc gttctgtaaa attaaatgga ttagaagaaa
     5101 gagttaagat tataaatgaa gatataaaat acatagacaa agtcttagat gtaaatggct
     5161 accatgtagt cacatcaaat cctccatata tgcatattga tggaataaaa aatccaaatg
     5221 ataaaaaagc aatatcaaga catgaagtaa ggtgtaattt ggaagatgta attagagctg
     5281 catccagatt ggttatgccg agaggaaaat tcttcatgat acatagacca acaagattgg
     5341 ttgatataat aactttaggt agaaaatata aattagagcc aaaggttatt caatttgtgc
     5401 atccaagacc tcaaaaagca ccgaacttag ttttggttca atttgtaaaa gatggaagac
     5461 cagagcttaa aatattggac ccattgtatg tttatggaga agatgggaat tatacaaaag
     5521 aattaaaagc aatatataat aataaagata taggagagtt gtaaatgagt gggaaattgt
     5581 acatatgtcc aacaccaata ggaaacctag aagatataac atatagaact ttaagaatat
     5641 taaaagaagt agacttgatt gcagcagaag atacaagaca tagtataaaa cttttaaatc
     5701 attttgaaat ctcaaaacca ctaactagtt atcatgaaca taataaggat tcaaagggag
     5761 attatttgat aaataaacta attgatggtg aaaatatagc tctgataagt gatgcaggta
     5821 tgcctggtat atctgaccca ggagaagaga taataaaaca agctatacaa aataatatag
     5881 agatagaagt attgccaggt gcaacagcat ttgtaacggc tttagttggc tctggtatgg
     5941 acacacataa atttgttttt gagggctttt tagatagaga taaaaaagtt aggaaagcac
     6001 aattggaaga attaaaggaa gaaagtagaa ctataatttt ctatgaatca ccacatagat
     6061 tgaaggatac actaaaagat atgcttaaaa ttcttggtaa taggaagatt tctataaata
     6121 gagaacttac aaaaaaatat caagaaataa taagagaaaa tatagaaact accataaaaa
     6181 tatttgagga aaaagaagta agaggagagt ttgtattaat agtagagggt tttcatggtg
     6241 agaagtcaaa gaaagaaaat tatgatgggc ttacagatag ggaatatgtt ttaaaactaa
     6301 ttgaaagtgg tatgaacaaa aaagatgcaa taaaaatagt ttgtaaagat agaaagttaa
     6361 agaaagatgt ggtttataaa caagttttag atttataaaa cctgaatctg aggtgatttt
     6421 atggaaatag gaaaaattat aatagagagt ttaaagaggg aagatacaca taaaatattt
     6481 atagaattag ataataagca tgaattagaa aaaataattc ctaaaataag ctgtatgaag
     6541 tctgtaggtg aatgtaaata tcatgtagta aattgttttg aacattctat aaatgcatta
     6601 aaagaattag aaatagtatt aaatgataag gatttttttc caatacatct aagaaaacat
     6661 gtgtcaaact atttaaacac atatactgaa aaaggaataa ataagttaca tgtattaaag
     6721 ctgggaacat ttttacatga tataggtaag cctgacagta tgactttaga tgaaacagga
     6781 agagttcact tcacaaatca tgaaaaaata ggtgctcaaa taattgacaa tatggggata
     6841 aagttggagt tatcaacaga gacatataga cttataagta aatatgtaag atatcatatg
     6901 atacttttgt ctctgtataa gaaaaatgat ttgagtagga aagaattaat taatgtattt
     6961 aacttggtag atgaggacac tataggtgtg ataatgcttg ggtatgcaga tatagtgtct
     7021 acagtaaaat tattgggcaa gagtggagaa gtgagtgtat taaagacata tatggaatat
     7081 attttaacaa attatttgta taagagtaat tatacacatg tataaattta gttaatataa
     7141 ttgagtttta gatgattaat taaagtctga aatacacgga tttcaaaaac ataaaagaag
     7201 ttgtctttgt tgtaaaatta tgaaagacaa ctttttttat atcaaaaatt gaaattttat
     7261 taaggatact taatggtcta ttaattaata aaccattagt atttaattta tattacaata
     7321 ttgattttgc ttgtattggc gttgatagta taacagaggt cttagtagaa ccaattttct
     7381 ttatagtatc aatcacattt tctagctcgt acatatcttt tacaattact tttaaaagta
     7441 agcaatcatc acctgttata tggtgacatt ctacaatcct agggtccttt gcagctgact
     7501 caataaattc tgtatatcca ttgcttggaa gagaaatatg gataaatgcc tttataactc
     7561 tacctaatga atctgggttg acaatagctt tatatccttc tataacacca gactcttcta
     7621 atcttttgac tctttctgaa actgcaggag aagttaaacc aactattttt cctaagtcct
     7681 tcatagaaat tctcccatca tcctgtaaaa tttctatgat tctgtaatct gtaacatcca
     7741 taaataaaca gtccccctta aataaaaata attaatgcta taaaactttt ttagaatcta
     7801 tcatttgtgt ttttaaatac caaagatttc tagataaatc aaccttataa atgtgtggtt
     7861 ttgaaagtct tttgtattgt tcccagatag tacttaattc atattgtaca tgatttttta
     7921 tttcaagaac agctttttta ttatatttgc atctgccttt tatatacaat ggtttaagaa
     7981 gctctttaac tttgtaattt gtaaatacct ttgttttcca tgtataagta ggatggaaga
     8041 tttctaaagg tttagactca tctataactt catcatgtaa cataatcaaa tctgcctctg
     8101 ccttattgtc ttcgttatat attcttataa ccttcttgta accaggatta tttatttttt
     8161 ctggattctc agaaatcttt atcttaggtt caagaactcc tttttcacaa gatgcagcta
     8221 atttataaac tcctccaaga gaaggtgaat cgaaagatgt aatgagcttt gtacctactc
     8281 cccatgaatt tatagtagct ccttctgctt taagtgatgt tatggtatat tcgtctaggt
     8341 cattagatgc agtgatgctg atatctgaaa agccagcgtt atctaattgt tttttagctt
     8401 ctttagataa ataagctaaa tctccagagt caagtcttat acccataggt ttgtatcctt
     8461 tttcagaaat atttttgaat acttttattg cattaggaac cccactgttt aatacatcat
     8521 aagtatctac aagtagtagg catttatctg gataaacatc agcatatgct tggaatgctt
     8581 caagttcatt atcaaatttt tgtacccaac tgtgagcttg tgtaccaatt ataggtatat
     8641 caaacatttt ccctgctagt acgtttgcag ttccagcaca accaccaact acagcagccc
     8701 tagctccata tatccctgca tctggacctt gtgcacggcg taatccaaat tcaaatacag
     8761 ggtctccttg agctgcaaag caaactctag aggctttagt agcaataagg gactgaaaat
     8821 taactattgt taacaatgct gtttcaataa gttgagcttg atataaaggt gccttaacag
     8881 taattaatgg ctcattaggg aacatgatag ttccttcctc aacagcatat atatctcctg
     8941 tgaatttaaa ttcttttaaa aattttaaga acttatcaga aaataaattt aagtttttta
     9001 agtactctaa atcttcatct gaaaaatgaa gattatctat gtattcaaca acctgatcaa
     9061 taccacaaac tatagtatac cctccatcac aagcgttttt tctaaaaaac atatcaaata
     9121 caacaatgtc ttcatgaata ttcttttcaa aatagccatt taacatagta agctgatata
     9181 aatcagtaag aagtgttaga tttctcatta tttctctcct taattattat taaatattaa
     9241 aaattgtaaa aactcccaca cttatactaa aacataatat agtacaactt tcaagtataa
     9301 agtattacct aactatcata aaattattta aagaggagga tgattataat aggaaaaata
     9361 tggttttata tggtatcgat aggaataata ggaagcttga tttctaaaaa tatgggagaa
     9421 ttaaataaag ttatattaag tgaaacatca aaaggcatag aatttgcaat aagtctagct
     9481 ggtatcatgg cactttggat ggggataatg aatatcgcaa aagagtcagg cttaattgaa
     9541 aagataggtc aaaaattaaa tcctataatg agaagattat ttccttctat tccaaagggt
     9601 cataaagcaa tgtcttatat agttatgaat attgctttaa atatgttggg tgctggaaat
     9661 ggtgcaacag catttggttt aaaagcaatg agtgaacttc aaactttgaa taacaagaaa
     9721 gatacagcca ctaatgatat gattatgttt atggtaatca atatatcatc tatacaaata
     9781 attccattta ctataatcaa attaaggatg gatatggggg ctcaaaatcc aagtgaaata
     9841 atttttacga ctttatttgc aaccataata tctacagcag tagcaataat aacatgtaaa
     9901 ttttttcaaa gaagatatag ataaagggga tgtagataca tggagatatt ttcaaactta
     9961 cttataccaa taataattct gtacatagta gtttatggta aatataaaaa aatagatgta
    10021 tatgatagtt ttgtaaaagg agcgattgat ggattaaaag cagcatggga tatcctacca
    10081 tatataatag gtatattttt ggcaatagga atattcaaaa ctggtaaagg tcttgatatg
    10141 ttagaatggc tatttacacc aatagcaaat atgatgagta taccaaaaga attaataggt
    10201 ctaataagtg taaaaccttt atctggaagt ggtgctttag ggatgtacag tgaacttgca
    10261 aatagagttg gaataggaag tttagtagaa aaaatgggtg ctacgattgt aggttcttct
    10321 gagactattt tttacacaat ggcaatatac tatggaagtc ttaagataaa aaatactaga
    10381 catacattat catgtgctat gattagccat gttgcaggtg tcatagcagc tgtttttatt
    10441 tgctatgtaa tatttgtata atttgttgac aatatgggaa aaattttata atatagtgat
    10501 tatatagaaa caaatagtaa tacaaattat aagctatgaa aagaagagta aataaaggtt
    10561 ttttctatca gagagctagg ttaggtgaaa gctagtgtca aaagctttat tgaagatggt
    10621 cttggagctt tttatctgag caagtgtgct agggtaaaac ggttggacac gttatagtct
    10681 actaaggtat ctataaaatg tttctacatt tcatgtgaaa cataactatt atagatgaat
    10741 tagggtggta ccgtgaataa tctcgcccct atgttaactc ataggaggcg attttttatt
    10801 atttaaatga aagaatcata tgattacgaa cgataaaaaa ttaggaggta aataacatga
    10861 gcaaaccgag tttttatgta acaacaccaa tatactatcc aagtggagga ttacacatag
    10921 gacatactta ttcaacagta gccgcagata caatagctag attcaagcgt ttctgtggat
    10981 atgatgtaaa gttcttaaca ggaacagatg agcatggtga aaaaattcaa aagaaagcca
    11041 tagaacaagg aatgtcagag atagagtatc ttgatggaat gataaaagat ataaaggcct
    11101 tatggaatac tatggatata tcttatgatg attttataag aactacagaa aaaagacata
    11161 cagaaataat acaaaagata tttactaaac tatatgagca aggtgatata tataaaggtg
    11221 aatatgaagg tagatactgt actccttgtg agagtttctg gacagaatct caattactag
    11281 aaggaaataa atgccctgac tgtggaagag aaacatattt agtaaaagaa gaatcttatt
    11341 tctttagatt atctaaatat gaagatagat tgaaagagtt atttaaagat gatagtttct
    11401 gttttcctgc tgctagaaaa aatgaaatgg tagcaaactt tttagacaaa ggattagaag
    11461 atttaagcgt aactagaacg acttttgact ggggtataaa agttcctttt gatgaaaaac
    11521 atgtaattta tgtgtgggta gatgctttat gtaactatat aacagcatta ggttatatga
    11581 ctgataatga tgaagaattt agaaaatatt ggcctgccaa tgttcaaata gtaggtaaag
    11641 aaatagtgag attccataca ataatatggc cagcactttt aatggcttta ggtctagaag
    11701 ttccaaaaca agtatttgga catggatgga tactgtttgc tgatgataaa atgagtaaat
    11761 caaagggtaa tgtggtttat ccagaaccta taatagaaag atatggaata gacacactta
    11821 agtacttctt attaagagag tttgcatttg ggcaagatgg aagttatact catagaaatt
    11881 ttgtaacaag aataaattat gatttagcaa atgatttagg gaacttaata agtagaactg
    11941 tagctatggt tgaaaagtac aataatggta taataccaac agcaaaagtt tcaactgatt
    12001 ttgatgctga cttaaaagag caagccgttt caactagaga aaactttgaa gctgaaatgg
    12061 ataaaatgca attccatgaa gcattagaga gtgtatggaa attagttaga agaactaaca
    12121 aatatataga tgaaactatg ccttgggcac tagctaaaga tgaaacgaag aaagatgaat
    12181 tagacacagt tttatataat ttatgtgaat ctataagaat aatcgcaaca ctgataaatc
    12241 ctataatgaa tgagactgct aataaaatat atgaacatat aggaataaaa ggtcaagatg
    12301 atataactac atgggaaagt acaaaaacat ttggtcttat tggagaaaat gtaaaagtat
    12361 ttaaaggtga acctttattc ccaagattag atgtagaaaa agagatagaa gaattaacta
    12421 aaatgttctc tgggaaacct ccagtagaag aaaaaccttt agagcataaa gaagaaatta
    12481 ctattgatga tttagacaaa atagagctta gagtagggaa aataataagc tgtgaaaaac
    12541 atccaaaagc aaataaatta ttagtatcac aagttaagat tggaccagaa acaagacaaa
    12601 tagtatcagg aatagctgaa tactataagc cagaggattt agtaggaaaa gaggttacag
    12661 tggtatgtaa cttaaaacca gttaaattaa gaggtgtaga atctcaaggt atgatattag
    12721 cagcaggtga tgatggagac ccatatgttt taccatttac acaaggggct aaagatggat
    12781 gcgaggttcg ttagtaggca attaaatagt aaggagatta aagatgttat ttgactcaca
    12841 tgcacattta aatgatgaaa gttttgatga agatagagat gaacttattg gttcattaaa
    12901 agataaaggt gtagatttgg ttgtaaatcc aggagcagat atagagacgt cgataacagc
    12961 aattgaactt gcgaaaaaat acgactttat atattcagca gttggggtac atccacatga
    13021 tgtatcaaaa ctagatgata cagcaataga aactctaaga aaacttgcaa ctgagaatga
    13081 aaaagttgta gctattggag agattggatt agattattat tatgattatt ctccaagaga
    13141 agaacaaaaa gagtggttta agaagcaaat tgaattagct aatgagctaa aacttccaat
    13201 aataatacat gatagagatg cacatggaga tacttttgat ataataaaga atactaaaaa
    13261 tcctgaaata ggatgtgtac ttcattgtta tagtgggaat gtagagttag ctagagaata
    13321 tgttaagatg ggttgttata tatcaattcc aggcacggtt acttttaaga ataataaaaa
    13381 aactagagaa gtagtaagag aaatacctct agaaagatta ttcatagaga ctgattcacc
    13441 atatatgtca ccagagccac atagaggaaa gagaaacaat ccttctcaag tatcttttgt
    13501 tgctgataag atagcccagg aaaaagggat atcttatgaa gaagtatgta gagttacaaa
    13561 agaaaatgca aagaaatttt ttaatataaa atagacaaat taaatcttat aatgatatat
    13621 ataataagtc attataagta taggatgaga gtgcctcaaa atgagttttt tagttcatga
    13681 ggcactcttt tttgtatgaa atcaaatata ttttcatcat ttcaacaatg aaaaagaagt
    13741 ttatcatatt ttgagacagt ttgtttgttt ttatttattt aattcatatt ttaaaatata
    13801 ttttcttaac attgttaaaa aagaatttac aagatattca ttagaattaa ctcaactcta
    13861 atattcactt aacttaagtt gtttattatc ttattaaaga gaagtaaacg aaaggtagat
    13921 taaggaggct aaattttgta tacagttgat agtactaaga tattgataaa tatgaactta
    13981 gagaaactag agaagatata ctcaatgata aaagatacat caagtatttc tataattaaa
    14041 gaacctaact tagctacagt aatggtaaga gctaatgaaa gtgtaaaaaa cacaacattt
    14101 aatttaggag agatattagt tactgaatgt agtgtaaagg tagatgaaag cttgggttac
    14161 ggaatagttt cagaaaacaa taataagaaa gccatttact tagcagttat agatgcagtt
    14221 ttacatagca ataatagtaa atttgatgag ttaaagaatt acataaataa aagtgtatat
    14281 gaagagaact taagatatga acaagagatt attgatgaat ttagcttgat taataaaacg
    14341 aaagttcaat tcaatgctat ggattaaata ggaggagaat catggtaaat tatacacaag
    14401 aaatatatag aaagatgcta gatagtgctt catttcctgg gaaattaaat aatatagata
    14461 atataaatat agaaaataat acaaaactat tgaatggagc aattggtata gcaatcactt
    14521 tattagacca agaagttacc ttctatatag aaaattatac tcaagaagat ataaaaaata
    14581 taagagcact tacaatgtca aatcaaatag attatagaaa atcagattat atatttttag
    14641 atattaattc agaagtggat atattacaga ttaaaatagg ttcttttgaa tatcctgatg
    14701 aaagtgcaac tatagtacat caagtaaatg atttatcaac gtactctcaa actaagtata
    14761 taaagcttga tttgagtggt cctggtataa aatctaaaaa tagtatatat ataaatggag
    14821 ttagagaaga atttatagaa agattatcta ccataaataa agattatcca ataggaatag
    14881 atttgatttt agtagacaaa aaaggagaac ttgcatttat accaaggtca tcaaaattaa
    14941 gttgggaggt attgtagaaa tggcttatgt agcagttaaa ggtggagaag aagccatatt
    15001 acaggctaaa aatatacttg agttttatag ggtaaaagga gatagcttgc ctatagatgt
    15061 aaagcagatt gaggaacaaa tgtcttatgc tgtagacaaa gtaatgtctg aaggaagttt
    15121 atacaacaag gaactatcag cattggctat aaaacaagct caaggtgata tattagaagc
    15181 ctctttctat ataagagctt taaggtcaac aatgcctaga attacatatt cagaacctat
    15241 aaatacagaa gagatgagaa taaaaagaag aatatcagct acttttaaag atataccagg
    15301 aggtcagtac cttggggcaa ctagagacta tgaaaataga atattaaatg tagacttatt
    15361 agaagaagat gaaaaaaaga atagagactt taaagatagt tttataaatg aattagaaac
    15421 agtagatgaa atccctgaat ttacaaaaat atctaatata tttagaaaag aaaacttatt
    15481 acaagaggaa gtttgtgaaa acaaatcgga agaagttatt gatgatatta caagaaatag
    15541 tttaagattt ccagtttcta ggtcagcaaa gttacaggca ttaactagag gtgaaagtgg
    15601 agtagtgtca ggttttgctt atgctattgt aagaggtttt ggtgatgaac atccatatat
    15661 aagtgaactt agaactggta atgtatttat aaaagtaaaa catcccatct atgaagatga
    15721 agaaataata ataggtgata tgctggttac tgaatgtgaa atagtttcaa aagcatcaca
    15781 aaataagact gaaaattcta aaactaattc ggaagataaa aataagatgt ctttaggata
    15841 tggtctttgt atggggaata atgagaataa ggctatatct atggccatac tagacaaatc
    15901 tttggagcca aatagtgaaa acctagcaca agatgaagaa tttgtattgc ttcatataga
    15961 tggaatagat tcattaggat ttatatctca ctttaagctt cctcactatg tagattttaa
    16021 agcggatgta gaaagaataa aatagtatat ggaggtaagt ttaggtgata tacaattatg
    16081 gttttttaga tgaaaataca aagaaggaaa taagaagaaa aatattaaaa gcagtttcaa
    16141 tacctggtta tcaagttccc tttggctcaa gagaattgcc aatagctaaa ggatggggta
    16201 ctggagggtt acaattaaca ctatcattaa ttggtaaaaa tgatgtagta aaagtaattg
    16261 accagggaag tgatgattct acaaatgctt gtaatataag gaactttatt gcaagtgtaa
    16321 gtaatgtaga aaccacaaaa gatacgttag aagctacatt gattcaaacg agacatagaa
    16381 taccagaaga aaaacttaaa tctaatcaaa tactaatatt tcaagtacca ataccagaaa
    16441 cccttagaat agtggaacct agtgaagtag aaacaagaag aatgcattca gaagaagatt
    16501 atagtaggat gtgggtatat ctttatgaag atatagttag atttaatgat atatctatag
    16561 cagttgaata tccatgtaaa gtaaatgata gatatttaat gaatccatca cctataccta
    16621 gatttgatat aaaaaaatta aatatgtcag acaatttatt cttgtttggt gcaggtagag
    16681 aaaaaagaat ttatgctatt cctccatata caaaggtaga acctttagag tttgaggatt
    16741 ataagtttga agaggaaaaa tttgaaggga aatactgtag tttatgtaac agtacaaata
    16801 catttttaga tgaagtatat gacagtgata ctaatgaaaa gtactatagt tgttcagata
    16861 caagttattg tgaaaaagtt agattaaaaa ataacagtat agatgtaaca ataggaggta
    16921 cttggaatga gtaaaataaa tgactctaaa agattagaaa gtatagacaa gcaagtatta
    16981 ataggaagaa atatcaatct tatttatggg gatgggtgtg aaaagtgttt tgaaagcaca
    17041 ggatatgaaa gtaataatat atgtaagtat tgtggttcta tagttgcatg taataatgta
    17101 aatttagaac tttatgaagg agaagtcctt ggaattgtag gtgaatctgg ttcaggtaaa
    17161 agtactttat taaaaatact gtttttccaa gaacatgcaa atagtggaga agtatatata
    17221 tcccattaca ataaagacac aaatatacta aagctttcag accaaaaaaa gagatacata
    17281 aagaatcatt ttatgggtat tgtgtatcaa aatccccacc ttggtttaaa tttagacttt
    17341 agtaatggtg gtaatatagc agaaaaatta ttgatggcaa atttatataa tgttgaaaaa
    17401 ataagagata gagccaaaga acttttaata aaaactaata tacctgtaga aagaatagac
    17461 cataaaccaa aatactttag tgggggaatg caacaacgag ttcaaatagc aaaagctctt
    17521 tcaaataacc ctccaatatt gctattagat gaggttacaa caggtttaga tgtttctgtg
    17581 caagcggaag tattggattt aataagagaa atccaaagag aacttaagat ttctatgata
    17641 gttgtatccc atgactttga cgttataaag atgcttgctg ataggacaat tgtaatgaaa
    17701 aatggaagtg tgattgagtc tggtcttaca gaccaaataa tggaggaccc acaacaccca
    17761 tatactcagc aacttataaa ttcattgcta taaatatcgt ttgtaaataa gcaaaatggg
    17821 ggtttttaat aaaatgaaag aaactatact tgaagtaaat aattttagca aagactttga
    17881 gttacatgca ttgaacaaga ctataacggc atgtaataat ataagttttg aagtttcaaa
    17941 aggtgagttt ttaggaataa taggtaaatc tggagcaggt aagtctacca tattaaaaag
    18001 tatctataaa acatatatac caacaactgg agaaataatt tttaattcag aaatatatgg
    18061 taatgtagat ctggctaaaa ttggagaccg agaaatgata aatcttagaa agaaagaaat
    18121 aggatatgta tcacagtttt taaagacact tccacgaata actgcaattg aactagtagt
    18181 acattcttta attgagagtg ggtttgaaaa agattgttcg tatgatatgg caaaagatat
    18241 acttaatcaa tttgaaataa aggaaaattt atgggatgct tatccaaata atttttctgg
    18301 tggagaaaaa ttaagattaa atttagctca agctatggtt aaaaagccta gacttttatt
    18361 acttgatgaa ccaactgcat cacttgataa tcaatctaaa atatatgtaa aagaaaaaat
    18421 gttggaactt aaaagtcaag gaactacaat gataggaatt tttcatgata tagagtttat
    18481 ggaaactgtt atagataaga cctttactat ggcaaaaggg actataagtg aaagtggggt
    18541 agcctagatg ataaaactaa ttcataatgc aaatattgta ttagaagata tgatattaga
    18601 aaatggccat ttaatcatag agggagatac aataaaaaaa gtatcaaatg ataagattga
    18661 ttacgcattt gactttaatg agatagatga aataatagat gcaaaaaact tatatataat
    18721 acctggtatt atagatattc acagtgatgc cattgaaaag gaaatagagc caagaccaag
    18781 tacattatta ccatttaata tggcatttta tgagctagat aaaaaactac ctgcaaacgg
    18841 aataactaca gtttatcatt ctatatcctt aggggatgga gtaggagtta gaagtattga
    18901 taactcttta aagatgatta aaaatattga ttcatataaa aatatagata gtaagagtat
    18961 aaatcataaa gttcatttaa gatatgaagt gttgtattat gaaggtttag ataaggtttt
    19021 agaactatta gatgaaaata aaatagatta cttatcaata atggaccatt ctccgggaca
    19081 aggtcagtat acaaatccaa cattttatag agagtatgct actaaagtgt ggggagttac
    19141 agaaaattat gtagatacat ggttagatga tttagtaaat ctgcatgata atttagattg
    19201 gaataaaata gctaatatta taggtatagc caaaactaaa aatataaatg ttgcatcaca
    19261 tgatgatgat acattagaaa aactggattt cataaaaaat ataggcataa atgtatctga
    19321 atttcctata actcttgaag ttgcaaaaca ttcaaagaaa ttgggtgttc taacttgttt
    19381 aggtgcaccg aatattgtaa gaggtaaatc tcataataac aatttaaaag caatggatgc
    19441 tatattggaa gattgttgtg acattgtgtg ttcagattat cttccatcag ctatgataaa
    19501 gtctatgtgt atagttgcag aaagaataaa taatttaaat aaagcagtca gtttatttac
    19561 ctcaaaccct gctaaagcag ttggtatata tgatgaaaga ggtagtataa aagaaaataa
    19621 aaaagcagat ttagttttag tagatattga tagtgaatat ccaaaggttg taaatactat
    19681 tgtaaacgga aaaacagttt ataaaaggga ggtataaaga ttgttaacag agttacactg
    19741 tcatacaagt atatcagata ataattttac tactgaagaa ataataaaaa aagcagcaga
    19801 aaagaatata aatatcttag caataacaaa tcatgacaca ttatctggat tggatgaagc
    19861 tcttgtgctt ggagaacatt atggagtaac aataatacca ggtatagaaa tttcagctta
    19921 tgattataaa aatagaaaaa gagttcatat tcttggatat aacatagatt taaacagtca
    19981 agaaataaaa aatttgtgca atccaatggt atgtgacaga cataaagcat ctgttaagat
    20041 ggtaaataaa attatagatt tagggtacaa aatatctctg gaggatgtaa agaaatactc
    20101 atcaaagact ggaatattta aacaacatat aatgaaggct cttatagata aaggatatac
    20161 agataatata tattcaagtc tatataaaga attgtttcat agaggaaacg gcaaggtata
    20221 tgtatcttta aaatatgtgg attacagaga tgcaataaaa gctataaaaa attctggtgg
    20281 aatttgtgtg ttagctcacc caggtcaaat ggataatttt agcgctattg aagagatggt
    20341 aaaagtagga ttagatggaa tagaagtata tcatccatct cacaacaaag aattagaaaa
    20401 agaatcgctt ttttatgcta agaagtacaa tctagtggtc acaggaggct cagattacca
    20461 cggattttat tctcaacata attatgaact tggaagcaag agcttaaata aagaagactt
    20521 atttaagttt aggttagctt taggaggggt attataaatg tcttttttac aagtaaaaaa
    20581 tgtaggtaaa agttatggac aagttaaggt gttaaaagac atatccattg atattgaaaa
    20641 aggggagttt atttgcttat taggtccaag tggttgtgga aaaagtacac tgcttagaat
    20701 aattgcaggg cttgaggata aacatggtgg aaaaataata ataaatgata aagatatgac
    20761 taattcacca cctgaaagta gaaattttgg tatagttttt caatcatatg cattatttcc
    20821 aaatatgaat gtttacaaaa acatagcttt tggactagaa aataaaaaca taagtaaatc
    20881 taatattgat aaaaaagtta aagaagttct agacgttgta gaattgagtg gatatgagaa
    20941 aaaatatcca tcacaattat caggtggaca acaacaaagg gttgcattag ctcgtgcgat
    21001 tgctttagag ccagactttt tacttcttga tgaaccttta tctgcattag atgcaaaagt
    21061 aagacttaaa cttagggagc agattagaag tttacataga aaactaggca ttacaactat
    21121 aatggtaaca catgaccaag aagaagctct ttgtttagct gataagatgg ttgttatgaa
    21181 tagaggagaa ataatacaag ttggaacgcc aaaggaggta tataaaaatc cagaaacacc
    21241 ttttgtagct gattttatag gaacaataaa ttttatagat gatggtatta ataagatagc
    21301 tattagacca gaagatataa aagtagagtc caatagagat tctaaagata aggatataaa
    21361 agtgggagaa attttagata tcgaatttag aggatttaac tatagaatta cagttgagta
    21421 tcgttctaag caaatgaaat tagatgtagt ttccaaagtt gcagaacaaa tgaaactgtg
    21481 cataggttct aaaataaact ttaagatacc taaagaagga attgtacaat ataaatcaga
    21541 gggatgtgct taatagctag gggtgaataa atgataacat caaaaaataa aagaagaata
    21601 aatgatagca caatacaaaa atatttcttg attttttcgg taattgtact aatagtattt
    21661 atattatttc cattagtatc attattaaaa aatgcattta tggatacaag tggaaatttt
    21721 ataggaattt caaactttag taaatatatt gaaaatccaa gtcttatagc atcatttaaa
    21781 aattcaattt ttgtatctac agtctcatct ttaatatcac tatgtttagc atttatatat
    21841 tcatacgcaa taacaagaac aaacataaag tttaaaaatt tttttaaaac tatgggaatg
    21901 tttccacttt ttgcaccaac tatgctatat ggtatttcac ttatatacct ttttggaaat
    21961 aagggaatct ttacaacaat gggtttggaa gtaccactat atggtccatt aggaataatt
    22021 atttctcaga ttctatttac atttccacaa gcttttttaa tattatctgt agcactttcg
    22081 atggctgatt acagacttta tgaagcagca gatagcttgg gcgctagtga atttaagaaa
    22141 ttttgcaaaa taacaattcc tggtataaag tactcgttac ttagttcatt ctttgtatcc
    22201 tttatacttg catttacaga ttttggagca gctaaggtag tcggaggaaa ctacaatgta
    22261 ctggccactg acatatataa acaagtagtt ggacaattta acataccaat gggagctact
    22321 gtctctatgg taatgttaat tcctgtacta atagcattta cattagataa gatggcaagt
    22381 aagaaacaag gtatgaccat aactgcaaag tctatgccat ataagattaa agaaaataag
    22441 tttagagata gagtattttt aatagtatgt agttttataa cattgctaat ttttatttta
    22501 atattcgttt cagttgtagg ttcagttata aagttatggc catataattt aagctttaca
    22561 atggaacact atacatttaa aggtattttg ggaagtggaa taaaaacata cttaagcagt
    22621 ttgaaaatat caactttaac agcaatattt ggaactatat ttgtattttt tagtgcttat
    22681 atgatagaaa aagtggataa gtttcctaaa ttaagacaaa cagcttactt tttgtcaatg
    22741 ataccaatgg ccttacctgg cttagtttta ggtatatcat atataacttt tttcaattca
    22801 gcaagtaatc cattaaattt tttatatgga agtacagcaa tattagttat agtaaatata
    22861 gttcattttt attcagtttg ttttataact tcaaattcat ctttaaaaat gttggataga
    22921 gagtatgaat tagttgcaaa atccataagt attccattct ataaagtatt ttttaatata
    22981 acagttccaa tgtctataac atcaatactt gaaatagtag tatattattt tgtaaattca
    23041 atggttactg tttcagcatt aatattttta tatacacctc aaactcagac tgcatcaata
    23101 tcaatactaa agttagatga gatagggtat ataggaccat cagctgctat ggcagtatta
    23161 gtgcttttaa caaatataat agtaagactt ttgtacgaat ttgtaacaaa gaaattaaaa
    23221 aataacacac aaaaatggca gcaaaaagat gttgcatagg ggaggtaaaa atgaaattta
    23281 agaagctagt ttctttagga gtattatcaa cacttttatt aagtgtaata acaggatgtg
    23341 gtactgattc atcaggttca gattcaaagt ctgatagtaa gacaaaaggg gatttaacag
    23401 tttacacagc aattgaagaa gatagtatag agccatattt agctacattt aaagagaaat
    23461 atccagatat taaattaaat attgttagag catcaacagg agatataaca gcaagattat
    23521 tagctgaaaa ggataatcct caagcagatg ttgtatgggg agttgcagcc acaagcttat
    23581 tggtcgcaga tgaccagggc atgctagaac catatgctcc aaaaggttct gaagaaatag
    23641 agtctgcatt taaagatgat aaagaagttc cttcttgggt tgggatagat gcttggatga
    23701 caggtataac agtaaacact aaagaattaa gtgataagaa cataccagtt cctagttctt
    23761 acgaagattt aataaagcca gaatataaag gtcttatatc tatgcctaat ccatcttctt
    23821 ctggtacagg gtacttaaca gtatcagccc ttatacaaat tatgggggaa gaaaaagcat
    23881 ggcagtatat ggataagttg catgagaata ttggtgtata cactcaatct ggttcagctc
    23941 cagctacatc agcagcttca ggagaatatc ctattggtat atcttttgga tatagaggga
    24001 taaaattaaa agaggaaggt tatccagtag aagtagtatt tccaaaagaa ggttctggat
    24061 gggatataga agcaaatgct cttgtgaaaa aagataatat aaaagaagca tcaaaagtat
    24121 ttttagattg ggctataagt aaagatgcaa tgaacgaata ctctaagaac tatgcagtta
    24181 ctactatttc tacaggaaat ccaataccag aaggattccc taaaaagcca ttagagcaaa
    24241 tgatagataa tgatttaaaa tcagcagcaa aaaatagaga agatatacta aataagtgga
    24301 tatcaaaata tgatggaaag acagaaaaag agagttaagt aaaactatac aaatagagta
    24361 aaaagaaaga atttttttaa gtagatttta ttataataat ataagttaag aataatatag
    24421 gtcaagaaaa atgaatattc tataaaaatg tatagagtaa gtataccatt cagtttgaat
    24481 ggtatatttt ttatttttgg agattaattt agaaatagat atactacagt ttaaaatagt
//
LOCUS       DAEMGG010000051        24464 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEMGG010000051 DAEMGG010000000
VERSION     DAEMGG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.051"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            256..1077
                     /locus_tag="KPK24_001936"
     CDS             256..1077
                     /locus_tag="KPK24_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG2299608.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            1098..2294
                     /locus_tag="KPK24_001937"
     CDS             1098..2294
                     /locus_tag="KPK24_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG2299609.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            2461..5859
                     /locus_tag="KPK24_001938"
     CDS             2461..5859
                     /locus_tag="KPK24_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG2299610.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5869..7542
                     /locus_tag="KPK24_001939"
     CDS             5869..7542
                     /locus_tag="KPK24_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG2299611.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7815..8009
                     /locus_tag="KPK24_001940"
     CDS             7815..8009
                     /locus_tag="KPK24_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG2299612.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    8178..8411
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8488..9885
                     /gene="asnS"
                     /locus_tag="KPK24_001941"
     CDS             8488..9885
                     /gene="asnS"
                     /locus_tag="KPK24_001941"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG2299613.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(10058..11233)
                     /locus_tag="KPK24_001942"
     CDS             complement(10058..11233)
                     /locus_tag="KPK24_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2299614.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11652..13016
                     /locus_tag="KPK24_001943"
     CDS             11652..13016
                     /locus_tag="KPK24_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2299615.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            13038..13529
                     /locus_tag="KPK24_001944"
     CDS             13038..13529
                     /locus_tag="KPK24_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2299616.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13801..14472
                     /locus_tag="KPK24_001945"
     CDS             13801..14472
                     /locus_tag="KPK24_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG2299617.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMKRLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14557..15423
                     /locus_tag="KPK24_001946"
     CDS             14557..15423
                     /locus_tag="KPK24_001946"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG2299618.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            15456..16958
                     /locus_tag="KPK24_001947"
     CDS             15456..16958
                     /locus_tag="KPK24_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG2299619.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            17088..17540
                     /locus_tag="KPK24_001948"
     CDS             17088..17540
                     /locus_tag="KPK24_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG2299620.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17700..18503
                     /locus_tag="KPK24_001949"
     CDS             17700..18503
                     /locus_tag="KPK24_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299621.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18561..19448
                     /locus_tag="KPK24_001950"
     CDS             18561..19448
                     /locus_tag="KPK24_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG2299622.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKDMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19467..20315
                     /locus_tag="KPK24_001951"
     CDS             19467..20315
                     /locus_tag="KPK24_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2299623.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20378..21067
                     /locus_tag="KPK24_001952"
     CDS             20378..21067
                     /locus_tag="KPK24_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2299624.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(21397..22587)
                     /locus_tag="KPK24_001953"
     CDS             complement(21397..22587)
                     /locus_tag="KPK24_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2299625.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYAWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLDDGIDTAGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSERGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEPKVILTPEQEERLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLRDSEIGYYIDTPKTKNGKRKIPLTE
                     RAYQALQRILKNRVKAQPLVVGGYSNFLFLNREGLPKVAGNYVGMVRGLIKKYNKYHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(22665..22868)
                     /locus_tag="KPK24_001954"
     CDS             complement(22665..22868)
                     /locus_tag="KPK24_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2299626.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            complement(23250..23498)
                     /locus_tag="KPK24_001955"
     CDS             complement(23250..23498)
                     /locus_tag="KPK24_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2299627.1"
                     /translation="MKHTFKKPSYYLLSQAMDGDEKAIEKVLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            complement(23502..23921)
                     /locus_tag="KPK24_001956"
     CDS             complement(23502..23921)
                     /locus_tag="KPK24_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2299628.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQQGLKRRKEKEISFC
                     ELPEIMVENLAVWDDYETDYTIFNVCGNDIRVYDDELAEALKQLSERNRETLLMYYFL
                     EMNNEEIAKKQNISRSGVFQNRHNSLALMRKLLKEKR"
BASE COUNT         9184 a         2924 c         4385 g         7971 t
ORIGIN      
        1 tggtgccttt gattaaacta agtttttata atttaaattt tgtacaataa gctatctttt
       61 attattcttt aaaatatata tttcatttaa ttatgacaaa taaagataaa aattaaatat
      121 tttagaggtg attttattta gatatagaat tttagaataa tactatacaa atgtattgta
      181 ttattatttt tatatcaaaa atagtaataa agtctatata catttagaaa atactgttta
      241 aaaggagtag gatttatgat aaaaaaagag atatctaact ttagaggaag tagtgattat
      301 gtagtatcac cagaattgat ggcatcagtt aatgttgcta ttgctcttga aaagccactt
      361 ttgattaagg gcgaacctgg aacaggtaaa actatgctag cacaagcaat ttcaaatgaa
      421 ttaaaaaaag atttagtaat ttggaatata aaatcaacta caaaagcaca agaagggctt
      481 tatgtatatg atactgttca aagactttat gatagccaat ttggaggaga aggtgtagat
      541 gatattagca agtacataaa atatggtaaa ctaggagagg cctttagctc aaatcaacaa
      601 gtaattcttt taatagatga aatagataag gcggatttag aatttccaaa tgacttgctt
      661 tgggaattgg ataaaatgga gttttacata aatgagacaa aagaaactgt aagagctaaa
      721 caaagaccaa tagttattat aacttcaaat gcggaaaaag aacttccaga tgctttttta
      781 agaagatgca tatttcatta tatagaattt cctgatagag acatgatgga agaaatagtt
      841 aaagtacatt ttgataaagt agaagaacac ctattggaac aagtaatgac tactttttat
      901 tggattagaa gtttaaagga catacaaaaa aagcctagta catcagagtt aatagactgg
      961 atacaagcat taactttgag tggaatgcct attgaaaaaa tagaaaaaga agttcctttt
     1021 gcaggaatac tacttaagaa taatgaggat attgaatcta tgcaaagaca cttatagtta
     1081 ataggagctg gataattatg tttattgaat ttttttattt attgaggtca agaggattaa
     1141 atgtatcttt aaatgaatgg atgactttaa ttgaagcatt ggataaagga ctgtgctatt
     1201 caaacttttc aaatttttac tatctttgca ggatgatttt aataaaaagt gaaagtgatt
     1261 ttgataagtt tgatgcagtg tttttggagt attttaaagg tatagaacac caagaagaaa
     1321 ttccagaaga gattatgaat tggcttaaaa aaccagatat agatttagaa gagtttgaaa
     1381 gattagaaca aaatccaaat ataaatcttg ataaattaag agctaaattg gaggaacgaa
     1441 taaaagaaca agattctcct cataatggtg gtaattattg gataggcaca ggaggcactt
     1501 cagagttagg acattcagga aaagggcaaa ctggtatacg tataggtgga aattctactt
     1561 atggtagagc tgttcttgaa gttgcaggag aaagaaaata tagagatttt agagatgatg
     1621 aagttttaaa tatgcgtcaa tttcaagttg cactaaagag acttcgtcaa ttttctacta
     1681 gaatagatgc tccaaagaca gagcttgatt tggataagac tattgaagag acttgtaata
     1741 atgcaggata tttaaagtta ttttttgaga agccaagaaa aaatacagtg aagcttttat
     1801 tgcttatgga ttcaggtggt tcaatgagag ggtatagcac tttatgtaat acattatttc
     1861 agtctgtaag taaatcaaat cattttaaag atgtaaagat atattatttc cataactgta
     1921 tttatgatag attatttaca acacctgaat gttggttaag taaatctatt aatactgaat
     1981 ggatattaaa aaatatagat aaaaactata aagttataat tgttggagat gcttctatgt
     2041 cgccttctga gttgcttcat attggtggta actatcgtgg tccatataat tacacacctg
     2101 gtatagagtg gcttaaaagg tttaagagaa agtacagtaa aattgtatgg atgaatccag
     2161 aacttagaga tggatgggat tcaattaatt attggtatca aactcaaaga atgatacaaa
     2221 gtgaatttaa tatgtttcca ttgactgtaa aaggtcttga gaaagcattg aaaaatttaa
     2281 tggtttcaag ataaagtaaa tataaatgat ggtatgattt attcaaaatt gtaccatcat
     2341 tatttatttt tttaatatac ctattataaa ttgtataact gaaacttaaa aaattatata
     2401 gtctaataca ttttagactc acacaaaaca tatacattaa taataatttt agaagtcgag
     2461 gtgattataa taaattatga attaattgta aaatacaatg gagatatatt gaggttagaa
     2521 gaagaattag gtgtatcagt agagatactt aattcatcat atgcaataat aacatcaagt
     2581 aatgaagaag atgtaaatat acttttaact tatccagaaa ttgaatttat agaaaagcct
     2641 tttatattac agactcagga tgtgcaaagt ttttcaagta caggaataac aggatttaaa
     2701 aatagaactg gtttaactgg aaaaggaact ataattggta taattgattc gggaatagat
     2761 tatactctac ctgtgtttag agatagtgat ggaaggtcta agatattata ttattgggac
     2821 caatctattc aaggaaatcc accagaaggt tttagagaag gaacattata tactaatgaa
     2881 gatataaata atgcaataga tggaagtatg tatataccta tttcaacaac tagcttacat
     2941 gggacacatg ttgcaggtat ttgtgcaact attgcaagtg atgccagaat aatagtggtt
     3001 agagttggaa atatacaaac cgatattttt tcgagaagta cagagtttat gagggcaatt
     3061 aaatttattt tagatagggc attagaatta agaatgcctg ttacattaaa tataagttat
     3121 ggaagtaatg aaggttcaca tagaggaaca tctttatttg aacaatatat agatgatatg
     3181 tgtttatttt ggaaaaacaa tatagttgta gcagctggaa ataatgcaga taaaggaggt
     3241 cataaaagga ttaggcttca aaataatatt acagaagaag ttgagtttat agttggggaa
     3301 ggagagcgta tattaaacat aaatatatgg ccagattttg ttgatgattt tagtgtacat
     3361 ttggtaaacc catcaaataa tcaaacacaa gcaatctctc taacttcagg tgagataagg
     3421 aatactttgg gtgaaactag aataactggt tatttttacc ctatagcacc gtattctctc
     3481 acaagaagag ttactctaca attaagttca aatacacaga taactcctgg gttatggaaa
     3541 attgtatttg aaccaataga tatagtaact ggtaatgtaa atatatattt gccaacttct
     3601 gaaggtctaa atagaaatac tagatttctg attcctactc aagagcttac tgtaacagtt
     3661 cctggaacag caagtagagt tataacagta ggtagtttta attcaagaac agatatagta
     3721 tctatatttt ctggtgaagg agatacacaa ttaggtgttt ttaaacctga tttattagct
     3781 ccaggtgaag atatagtatc ttttttacca ggaggtacaa gtggagcatt aactggaact
     3841 agcatggcta cacctcatgt tacaggggtt tgttcattgt ttatggaatg gggaattgta
     3901 aatggaaatg atttattttt gtattcacag aaattaaggg cattacttct aaagggagca
     3961 aggagattaa gtaatcaatc ttatccaaat aattcatcag gatttggttt tttaaattta
     4021 tcagatatag atttgtacac tctatctaat ataaatcaag atttagaaac agaggatatt
     4081 ggatatagaa gtattaataa gtcatttaaa gatgaggaaa atagttataa gtttatagat
     4141 ggttataata tgcaaataca taatgactta gagaatgaaa tttatataag taaaaatgca
     4201 agtagacaat caggaatact atcaggaata gatatagtcc atactcctga atttgaagaa
     4261 gaactagctg ggttaggtat gagtcaaagg ttttttaaga taagtgattc attaggtgta
     4321 ctatctataa ataatacaga ctataatagc atacaacgag ttttgcaact tccttcaatc
     4381 ataagaactg tatcgactac aaagatgaca ttacttggag aaattaatcg aggtactttt
     4441 ggaggtgtag ttgcaacaga agaaatgggc gtaaatttct ttaaaaataa tcctaatatt
     4501 aatataacag gaagagggac tctaatatca attgcagata caggaataga ctatctacac
     4561 ccagatttta tttatccaga tggcacttca aaaatagtat atttatggga ccaaacaaaa
     4621 gaaggaactc ctcctgatgg tttttatatt gggacagaat atacacgaga agatataaat
     4681 cgagctatcg cagaaaatga ccctagtcta tctcaggatg aagttggtca aggaactatg
     4741 ttgagtggga tatgtgcagg gcttggaaat gtaaatagtg aatatgcagg tatagcagaa
     4801 gattctgagc taataataat aaaactcggc aagatagatg atttttataa tagtgcaatg
     4861 ctgtttgctg catctcagta tgcatataaa aaagcttttg aacttggaag accattagtt
     4921 ataaatatgt ctttaggtac cagtagccta gcaggtttaa ccaataggag taatagtgaa
     4981 aaagcgttct ttactagagg gttatgtata acggcaggag caggaaatga gggcaataca
     5041 cagacacata catctggaat aataccatat gttggaggtt ctgttgaagt tgaattagag
     5101 ttaaatgaag atgaggaaga attatcatta gaactttggt taaatagacc agataaagct
     5161 gatgtaataa tagtatctcc cactggtgaa gaaagtaaaa gtgttggaat ttcaaattat
     5221 aataaagtaa caggtttatt tgatttagag gggactgaat attctataac atatatatac
     5281 ccaacaactt tttctggaca gcaatttact aatgttactt taaagaatgc aaaaagaggt
     5341 gtatggaaga ttcgattagt aggagtttat attataacag gaagatataa cttatacctt
     5401 ccaaatagag aacttttaaa aagtggaact agatttagag aggtagaccc tttttacact
     5461 ataaattatc ctgcgattca agatgattta ataaccatag gagcctacaa tactattaat
     5521 ggtagtttat ggcaaagttc atcaagaggg ccaactattg aagacaggtt aaaaccagat
     5581 atagtagcac caggtgttaa tataatagct gcttatcctg ggaacacgta tgcaactata
     5641 actggtacag cagcagcaag tgcacatgca gctggtgctg ctgcaatgta ttttcaatat
     5701 acttttgttg acggtagata tccaaaccaa gcatatgtgc aaaaaataaa aacttttatg
     5761 caagctggtg ctagaaaaga ttcaaacaca gtatatccaa atacaaattc tggatatggg
     5821 ctcttggatg ttagaggaat gtttgatgtt ttaagatagg agggatttat ggaaaaatct
     5881 tattgtataa tttatcaagg tgatatagaa tctgccctac aagaaaatgg aataaatagg
     5941 tatatggttt taaatagtca gcttgcagta atatatgtac ctttagattt tgatgaaact
     6001 attttaaata atataataca agttgcttgg tgggaagaat ctgaaccaat gagcagtcta
     6061 attgaaataa ctaataatgt aaataatgga gaaaccatta caacagcagc tgaaactgat
     6121 tatatttatg aaaatccata taatgatata acagggagag gaattttatt agcagttata
     6181 gattctggga tagattattt gcatcctgat tttataaatg atgatggaac tagtaaagtt
     6241 ttatatttat gggaccaaga agcaaataca aatccacctc cagaaggttt tatttttgga
     6301 agtgaattta caagaagtga gttaaatata gccataaata gaaatgatgg gagtttgagt
     6361 caagataata taggtactgg aactttagtt tctggtatat tagttggaaa tggaaggata
     6421 aattctcagt acagaggtat tactacagaa tcagacttaa tagtagttaa attaaaatca
     6481 tatacagata cttattatgc tggaaggata aattacagtg tttcagattt tttagcagct
     6541 ataacgtatg ttactaacat agcaagaaca gaaaataaac cactgataat aaatttgact
     6601 attggagtta agtcaagtgc tgtagcaact acttcaatac tagatacttt taatatattg
     6661 agtagtgctg gtgtggtagt agtgagtggt gcaggaaatc aagggaatac agatattcat
     6721 tattctggca gatttagcag tgtgggagaa gttcaagatg taataataca agatggagat
     6781 gactatgctc ttgatataac actaaatacg aatggtccag ataaagtggg agcacagata
     6841 atatctcctt caggtgaagt tagtcatgat ataaggtatt ctccagactt ttatatatat
     6901 agaggaaaat ttaacttaga aaatactact tatgcaatga gatttattta tccatatatt
     6961 acctctggaa aagaaaattt ggagataaga ttaagagata taaaacctgg agtatggatt
     7021 ttaagattga cttcagaact tataataaat ggagaatatg acatatactt gccaaataaa
     7081 aatttgatag ctccagacac aaggttttta gaccctgatt cagtagctac tataactatg
     7141 tatgctgcta gtgatgatgt aattacagtt ggtacattta acaataagac tgatagtatg
     7201 tggattggtt cttcgaaagg accaattaga ggaagaggaa ttaagccaga tatagtagct
     7261 tcaggtgtag atataatatc cacatataaa aatggaacat ataatacagg aacaggaaca
     7321 ggagtgagca gttctatagt cacaggtgtt ttagccttat tgatggaata cttagaaaaa
     7381 caggataatg taccgagatt atcattgttt acacaagttt taaaaacata tttaatattg
     7441 ggtgctacaa aacttgagat atatacatat ccaaatgtat cgcagggata tgggatttta
     7501 aatttaaaaa atacgattca acagatagca aatactctat agataagaac ctatgtaaaa
     7561 ttataattga ttattagttt aattgtatca aatactttag atagagtaga aaaatagcat
     7621 aaaaaataaa tatgaaaatt aaaaaacagt tttatatttt gaatacacaa agtaaatatt
     7681 ttgtatataa ataggtataa attaagtatt gaaaagatat gatgttaatt taaaaataat
     7741 tataatttag acttatgaat tttatttgta aaattgctaa aactattaca aaataaataa
     7801 tagaggtgat agttttggaa agtaaaaagg caagagcatt aaaaggtaaa gataataaaa
     7861 gacttaaaag ttcactttta gttggaaatg agtcgacagc agcatgggct gatgttgaaa
     7921 aattaaaacc tcatagtaaa gtttcaatac cttcaattaa gggtgtcgaa gaagcaaaag
     7981 actgggttga caatggaagt aaattataaa attttggaaa ttgaagtaat aaagaagtaa
     8041 tgaagataag tatttattat cattatgtaa ggtagaatta ttatatttta aataacaatc
     8101 agtatcaatt atataaattt attgactaaa aatttataaa ttggtataat tttgttaatt
     8161 agcagaaata ataaaagata aatactgtga agagaagagt agatataaaa tgtagttcta
     8221 gcgagttgag gatggtgaaa gctcaatacg aggtttatat tgaagaacat cttggagttt
     8281 ctaatcgaaa gcttatttag taagtagact tagacggaag gcaccgttat aggctttaag
     8341 cattgcttaa ctaagaggtc attatgacaa ctagggtggt accgcgagag taatagtctt
     8401 tcgtcccttt atatagggat gaagggcttt ttttattgaa attatattta aaagaaatta
     8461 ataacaatta tactaggagg atttaaaatg caaaaggaat ttataacagt aaaagaattg
     8521 tacagagaaa aagaacagta catagggaag actgtaaaag tagctggatg gataagaaca
     8581 tctagagtat ctaaaaattt tggatttata gagctaaatg atggaagctt ctttaaaaat
     8641 atgcagatag tatttgatga gaaagtagaa aattttaaag aggtaggaaa attagcaata
     8701 agctcatcta tacttgtaga gggagagtta gtatctacag aaggggctaa acaaccagtc
     8761 gaaatacatg caaaaagcat agttgtagaa ggggaatcag atagttcata tccactacaa
     8821 aagaagagac atacgcttga atatttaaga acgatagcac atttaagacc aagaagtaat
     8881 actttttcag ctgtatttag agtaagatca atagctgcct atgcaataca caaattcttc
     8941 caagaaagaa actttgtata tgcacattct ccaatcataa caggtagtga ctgtgaaggt
     9001 gcaggagaga tgtttagaat aacaactatg gatttaaatg atattccaaa aactgaagat
     9061 ggcaaaatag actatactca agatttcttt ggaaaagagg ctaatttaac agttagtggt
     9121 caattaaatg ccgagataat ggcactttct tttagaaacg tttatacttt tggacctaca
     9181 tttagagctg aaaactctta tacaggaaga catgcttcag aattttggat gatagagcca
     9241 gaaatagttt ttgcagacct tgaagataac atggaacttg cagaagatat gataaaatat
     9301 gttataagct atgtattaga aaatgctcct gaagaaatgg aattttttaa tagttttata
     9361 gataaaggat tgttagaaag gttaaataag atagtaaact ctgagtttat tagaataacg
     9421 tatactaaag cagtggaatt attaattgaa tctgggcatg aatttgagta tcctgttgag
     9481 tggggatgtg accttcaaac tgagcatgaa agatatataa cggagcaaat atataattgt
     9541 ccagtgtttg ttacagatta tccaaaagat ataaaagctt tctatatgag aatgaatgaa
     9601 gatggtaaaa ctgttagagc aatggattta ctagttcctg gagttggaga aatcgtagga
     9661 ggttctcaaa gagaagaaag agaagatgta ttgttagata gaattaatga aatgggatta
     9721 agagaagaag attattggtg gtatttagaa cttagaaagt ttggtactgc tactcactct
     9781 ggatttggtc ttggatttga aagaataatt atgtatatga caggtatgtc taacataaga
     9841 gacgttatac ctttcccaag aactcctaaa aatgctgaat tttaataatt atatataata
     9901 aaacttatat aactttaagt ataaacacta aaatgacttg catatttatc tattttagag
     9961 atatgcaagt tatttttatt gtactataaa tatataattt gattactttt attttgcgat
    10021 ataatactta taggtttatg taatagtatg taacctttta ataaaatgca acacaattac
    10081 gtccctgttc ttttgcagta tatagagcct tatcacaatt acttataaac tcatttaatg
    10141 ttattgtctt tccaattttc tttgtataaa ctccaatact aacagttaca cattttaaag
    10201 gaacatcatc acgctcaaaa ttgcaggact ctataatttt tcgtaaacat tctgatttaa
    10261 gtttagcatc tgcctcatta cagttaaaaa tacatataaa aaattcctca ccaccatatc
    10321 gtatagcatg ttcattttca gaacttgtaa aatctaaaat tactttggca atattttgta
    10381 gacaatagtc tcccttttga tgtccataat tatcattaaa ttgtttaaaa taatctatat
    10441 ctatcatcat tccagttata ggttcttgat ttgcatggct gattaatttt ttttgtagaa
    10501 tatcttcaag gtatctacgg ttgttcattt tagtaaggtc atctgtcata actttgtcgt
    10561 taagtaaatt atttatatga gatatttctt catattgttg actaattaaa agtttatcat
    10621 agtaagtaaa tacacgattt ctatatagca ttgtagaaac aaaaatggca agggatgtta
    10681 taaaaaatgt gttgacaata ttactaaact cattgcctgg tatagtttga atcccaggta
    10741 agaataaatt aagaaacaaa aagcatcctc caaaaactat tactgattct ccaaatttta
    10801 atactgagaa agctgcagct gctaacataa tataactaaa tacacttagg tcatttccac
    10861 ctaactggtc aagtagagta attacacaac cccataggca gaaaaaagtc acataaatta
    10921 tccataaatt tatataaaac gtacagcaat cctttttttt attccaaagg taagaaaata
    10981 caaacataaa gacagaagat acacaaaata gtgttacata catataaaaa taccattggc
    11041 gtcttggtgt gacaaaaggg cctccttctc tagctgagat tgatatcatc ataattattt
    11101 gagatattac tattattatg caaatgattt tacccgtatg ataaagataa ttgcagagtt
    11161 ctttctcaac attgtctttg tgttttttgt ctagaggaaa tccaaaaaat ttttttattt
    11221 tttctaagtt catataagcc tccattaaaa ataaagcata aaaataagta ttcctttcta
    11281 tcattagata tttggaatac ttcgtttatg atttaattat atcaaaatat attgtgtgta
    11341 acaatctaaa attgtgatag ataaaattta ttagataatg tcgagtaact ttattgatta
    11401 atactttatt ataattaaga tttacagata gtttactact gaaaataaag aatagaataa
    11461 atttaaatta agcataataa aatataaata ataaagttcg cattaacaaa ctatttaaat
    11521 atcaagacct gttaaaaaac aacagcaata aacaaataaa atagataaat atatccaatt
    11581 aaggtataat taagttttat atgatatagt tatatttgat aatttacaat agtaaaactt
    11641 ggagggaaaa aatgggtgaa aagaaaataa ataatatgaa ttattcacat aataattatt
    11701 ccaaaaagac aggtgaacca actagaaaaa taaaaaatag ccatatcact cttaaaagta
    11761 aagaagaaaa aatttgttct agttgcaata aagaaaataa tactaaggat aattactgta
    11821 aattttgtgg aaatgaactt tatgaaatag catcattaag accacttgaa acaaaactag
    11881 acttgaaatc taaaataaaa gagctttcat atcatgcaaa taaaagaggt gtatttttaa
    11941 caacatttac aaccatattt atattgttta ttattgcatt gatatttaaa gctattataa
    12001 ctattcaatt taatgatatt agttatttag taaatccagc acatataatc ctagctctca
    12061 acttgggtca aattagtgta tctatgtcta ccatgatggg ttctgggttt ataaatgcca
    12121 atataggttt gttgatatta cttataatcc ctattttggt attattaata tcaaacctaa
    12181 tttttatgag aaaaagatgt agagattcaa aaactgtatt agcaaattct ctaggagttg
    12241 gaatttttta tggattagtt ttagctattt tatctatttt tactaatgta aaaactagtt
    12301 cacatagtat gttagaatat ggatatgcat tagaatatag ttatgaattt tttagtgttc
    12361 ttttaaatgg attcgtatta gggtttatat gtacatacat aactacttat aaaaaaagtt
    12421 atgaaaaaga aaatatgtat ttatctcttt tttcaaattc tataagaaca ttcatattag
    12481 gttatgtttt agtattagta atactattgg tactaactat atctgatagt agttacttaa
    12541 atgaactaga tatgagtagt tattcaaatg gattaaattt atttactata ctcccacaaa
    12601 tagctagtta tatgtgggct tttgcaaatg gaatttcagt aaccataata aactcaacag
    12661 tatcaatgtt tactttaagt tcttcatctt tatttggaga tactaaatta atgttttatg
    12721 caatgggagc attgtctatg ttaatattat tattaaatgg atacaagcta agatttaagt
    12781 ataatacaga tagtataaga cctataatag tatttagtat atattatgca tttttgatgg
    12841 gaatattggc cttatttagt acatttatac tagatagtaa tattaatttc tttaatacaa
    12901 ctaattatgg aacaacttta ataatgcaat ttaaagtctt acaagctatt gtaatttcat
    12961 ttgtatatag ttttgtgata tctttaatag gatataagct aaattcagct gattaaatat
    13021 gattggaggg aatttctgtg gaaggaaata ctaataataa agtagatgat aaattttcta
    13081 aggttaagaa aaagtttggt agtggaaaat ttaaagttga attatataaa gaaaaatcaa
    13141 aattaaatag agagattgat gatattcaag aaaagaaagc aaagatattc ttggaaatgg
    13201 gaatactgac atatcaaaaa attagagaag attctattaa tgataatagt tttgataaat
    13261 tctgtgatga gttactagaa cttgataagt taatttatga aaaaaatatg gaaattaatg
    13321 aaatggaaat ggcagaaagt aatgtaactt gtgagtgtgg ttatgttgga aatgtaaatg
    13381 ataaatattg tgctgaatgt ggtaaaaaat ttgaattgga aaatgaatgt gatgatttta
    13441 taatttgtgg ttattgtgaa agtgagatag atacagaggc tgaattttgt ccatgctgtg
    13501 gaagaaaaat tataatggat ttagaataat attgggaaac ctatgtatag tatacatggg
    13561 tttttttatt gaaattattt aaatggactt ataacagtag ttatataaaa tgaaaataaa
    13621 caccaaaaaa atgaaataaa atttctttta aatgtaaaaa acaagctaat atataaaata
    13681 aatcagtaaa aatatagatg cgagaaaaaa tatgaaaaaa tattgcaaag aaaaagtttt
    13741 catgttatta ttaatacata aatcaagaaa ataaatttca gaaacgaggt aatttaaaaa
    13801 atgctagata aggtaaaggg aagattaata gtatcatgcc aagctttaga aaatgaacct
    13861 cttcatagtc cttttataat ggggagaatg gctaaggctg ctatggaagg tggagctgtt
    13921 ggaattagag ctcaaggagt tgaagatatt attgaaataa aaaaagttac tggacttcct
    13981 gttataggta ttataaagag aaactatgaa gattcagata tatatataac gccaactaaa
    14041 aaagaagttg atgaactttt aacaacaggg tgtgaaatga tagcattaga tgctaccaat
    14101 agagttagac caaataatga agacttaaaa gaacttataa agtatataaa agaaaacggt
    14161 gttctagtaa tggcagatat ttcaaattat gatgaggcta tcaaagcaca agagtacggt
    14221 gtagattgcg tttcaactac actatctgga tatactccat atactaaaac attagaaggt
    14281 ccagattttg tattaatgaa aagattagtt aaagatttag aaatacctgt aatagctgaa
    14341 ggaaaggtaa atactcctca agatttaaaa aaggtctttg agttgggtgt tcattcatca
    14401 gtagtaggtt cagcaataac aagaccacaa ttaataacag aaaaatttgt taaagcaatt
    14461 gaaattaatt aaaaattaat ttataaatta aaaaattatt atatttaact atatttgaaa
    14521 attaaaattg gaggtaaaga tatgagaaca acagatatga agggaattta ttcagcatta
    14581 ttagtttcat ttgataagga aggaaatata aatgaaaaag ggttaagaca aataattaga
    14641 cacaatatag atgtttgtaa agtagatggt ctttatgtag gaggaagtac aggggaaaac
    14701 ttcatgcttt caactgatga aaagaaaaga atatttgaaa tagctaaaga tgaagtgaaa
    14761 gaagaaataa aattaatagc tcaagttggt tcagttaatt taaaagaagc tgtagaactt
    14821 gctaaattta caactgattt aggatatgat gcaataagtg ctgttacacc tttttactat
    14881 aaatttgatt ttgaagaaat aaaacactat tataatacaa taataaattc tgtagataat
    14941 agattaataa tatactctat accattttta actggtgttg atatgagttt agaccagttt
    15001 ggagaattat ttgaaaatga aaaaataata ggtgttaagt ttacagcagc agatttctac
    15061 ttattagaga gaatgagaaa aacattccca aataaattaa tatttgcagg atttgatgaa
    15121 atgatgttac cagctacagt tttaggtgta gatggggcaa ttggttctac attcaatgta
    15181 aacggtgtca gagctagaca aatattcgaa ttaactaaga atgaaaaaat atcagaagca
    15241 cttgaagtac aacatgtgac taatgattta ataacagata tattaggaaa tggcttatat
    15301 caaacaatta aattactact tgaagaacaa ggtgttgagg ctggatactg tagacaacct
    15361 atgaaagaag caactgatga aatgaaatca agagcaaaag aaatatacag aaagtatttt
    15421 taataaaata cttttagtat gggaggatta gtcaaatggt aggatttact tggatagatt
    15481 ttgcaatact agtggtttac ttattggcag ttttatttgc aggattgtta ttttcaaaaa
    15541 aggaaatgaa gggaaaagag ttcttcaaag gtgatggaac tattccgtgg tgggttacat
    15601 cagtatcaat atttgccaca ttattaagtc caatatcatt tttatcatta gcaggaaatt
    15661 cttatggtgg tacttggatt ttgtggtttg cacaattagg tatgtttata gcaatacctt
    15721 tgacaataaa atttttctta cctctatata gtaggttaga aatagataca gcatatgaat
    15781 acttagaaat gagatacgaa agtaaaggtc ttagagtact tggagctttg atgtttataa
    15841 tatatcaaat aggacgtatg tctattataa tgtatcttcc atcaatagtt ctggctggtc
    15901 ttacaggaat acctgtaaat gtactgataa tagttatggg tgtaatagca ataatttact
    15961 catatacagg tggattaaag gctgtcttgt ggaccgattt tatacaagga atggtattaa
    16021 tagtaggtgt tacagggaca ttgttctatt taatagcaag tataaatggt ggttttggaa
    16081 cagtaatgga tactcttaca actggacaca agttcttagc aagtaatgaa gttatgtttg
    16141 ataaaaatat actatctaca agtgcattta taatatttgt tggagctggt cttaatactt
    16201 tttcatcata tgtatcgagt caagatatag ttcaaaggtt tactacaaca actgatataa
    16261 agcaacttaa taaaatgaca tatggaaatg gtgttttatc aatgggactt gcaacagtat
    16321 tttatctaat tggaacttgt ttatttatat actatactca aaacccagat ttagctcaaa
    16381 cagttcaaca agaccaagta tttgcatcat atatagctta tgaattacca gtgggtataa
    16441 caggaatact acttgcagcg atatatgcag cttctcaatc aactttatcg acagggctta
    16501 attcagttgc tacaagttgg actttagata tacaaactat cataacaaaa gatataagta
    16561 tggaaaagca aactaagata gcacaatata tatcattagg tgtaggtata ctttctatag
    16621 ctgttgcaat agtattagca aatggagaaa taaagtcagc atatgaatgg ttcaatagtt
    16681 ttatggggct agtactaggt gttttagcag gaatatttgt acttggtgca ttttgtaaaa
    16741 aagcaactaa aatgggagca tatgttggat tcatagtatc agcaatttta gttgtttatt
    16801 taaaataccg tatgccagaa gtaacttctt gggcatattc tctaataaca atcacgactt
    16861 ctgtagtggt aggtacaata gttagtgcaa tagaagctaa agttaaagca aaagtatctt
    16921 taccaaaagc tgagactact gtttattatg aaaaataata tttaaatgat aaattgtctt
    16981 atagagtgat tagataaata atttagttac tctataagat tgtataatat actttgtata
    17041 caaaagtata aaaatagact ttataattat atagggagat agtgcttatg atatttggaa
    17101 atataaatca tagtaagaca tattcaacat tgcatgaaga tttacttaga tgttttgaat
    17161 atgctaaaga taattcatta atagattatg aaaaaggtac ttatacaata gatggtgatg
    17221 atatttttgt gaatattgta gagtataaaa cttgtgaaaa agaagataga ttctgggaag
    17281 ctcataagaa gtatatagat gttcatttga tgcttgatgg atgtgaacga atagatataa
    17341 attttataga aaatttagaa caaaaaatat atgaagaaaa gggcgatttc ttgcctcttg
    17401 atggtaaaaa taatggatac atggaactta gaaaaggtga ttttttagtt tgttatccgg
    17461 aagatgctca tatgacagga ataaaagttt tagaaaaaga aaatatcaaa aaagctattt
    17521 ttaaggtttt ggttagataa tgataattta tataaccact agggctgaaa atagtgattt
    17581 aactttcagc gattagtgag acttcgtaaa ttttagttag attactattt tcagccttag
    17641 ttttttatga gaaaattaaa aataattaaa agcttaaaat tttgtaaagg agttaactta
    17701 tggaaaatat agatgtattc ttattaatag gacaaagcaa tgctagagga ttgggaaatc
    17761 caaaagagag catcattcca aatgaaaatt gttttgaata tttgagtact gatgaaatta
    17821 taaatatgag atgtgagcta gagacttcag aaggtgatgg tactatagca cctgcatttt
    17881 caaatgaatg gaataagctt actggaaata aagtttgttt tattcacaat gcaaaagatg
    17941 gttcaagaat aaaaaactgg aatcatgata ataactggtt tctaaatgac actatagaaa
    18001 aatttaatac aggctgtact accttaagta aacattataa tatagaaaat aaatacgtta
    18061 tttggattca aggtgaaagt gatgcaaaat atggtagtga tgtattgtac tataaagaaa
    18121 gtctaaaaaa aattgcgtat aggcttaaag aagaatgtat gattgataaa atgtttgtaa
    18181 gtttaactgg ttattggctt ggagaagatg aatattttat tagaacaaga cgaattgcag
    18241 cagcacagga gtcggcttgt agtgaatgtg atatattatg tgtaggaagc aaaattgcta
    18301 tgcagtttca tgacagagga ttaactattg atgatgttca ttacactcaa gaaggtctta
    18361 atatgttagg agaggactta tgtaaaaata tttataagta tcatataacc aaagaaaaaa
    18421 gtgtattaag tgatactata gacttatcag aagcaaggaa atatatctgt gaattggaaa
    18481 agataagtaa aagatttgtg taatataaaa ttttaaatag agttgttaat ttataaaaat
    18541 aatttgtatg gagatatgtt atgaaaaatt acgtttgtat agatgttgga ggtacttcaa
    18601 taaaatatgg ttttattaga gaagatggat ttataattga taaatcaagt cttgatactg
    18661 aagcaaagga aaaaggtgga gaaggaatat tagccaaaat aaaagatata gtaaaaaaat
    18721 atatagagga aaatgaaata agtggtatat gtatatcaac tgcaggtatg gttgacccag
    18781 ttgaaggtaa aatactattt gcattagaag aattaattcc aaactataag gacatgcaat
    18841 taaaaaaaga agtagaaaaa gagtttaata taaaatgtga agttgaaaat gatgttaatt
    18901 gtgctggact tggtgaaatg tggcttggag caggtagagg tgcaacatct tctatatgtt
    18961 taacaatagg tactggaatt ggtggatgta ttataataaa taataaactg ataaatgggt
    19021 ttagtaatag tgctggtgaa gtaggatata tgaatgtaaa tggaagttct tttcaagaat
    19081 tggcatctac tagcagtcta attaaaaaag tagcaaaaat aaaaaatttg aatgaaaatg
    19141 acttaaatgg aaaaataatt tttgatttag caaagaataa tgatgaagat tgtttaaaag
    19201 aactcgataa tatgataaaa tcattagcag ttggaatcgc aaatatatgt tatgtaataa
    19261 atccagaagt ggtaatttta ggtggtggga ttatggcaca agaaaagttc ctaaaaccaa
    19321 aaatagacaa agcattaaag gaggttctta tagagagagt ttacaaaaac actaatatag
    19381 aatttgcaaa gagacaaaat gatgcaggaa tgcttggagc attatataat tttttaaaca
    19441 aaatataaag aatagaaggg tttttaatga gtattttaga acaattggaa tcaccaactt
    19501 ttaaagtcac aaaatcagat aaattattaa tagcttatat aaaagaaaat atagatgatg
    19561 tattttataa accaatatca caaatagcta aagagagcaa tattggtgaa gcaactataa
    19621 ctaggtttgt aaaaaaaatg aattttaatg gattacagga ttttaaagtt actttagcac
    19681 aagaaatatc aactatcaat aaaaaaaata ttataaataa aaatatacaa aatgatgagc
    19741 cagctttgga tacagcaaaa aaacttttaa gttcaaatgt aaccacttta gagaacacag
    19801 tagagataat caatagtaag gatgtacatg actgcgcaag gcttataata aatgcaaaga
    19861 aagtttactt cataggtatt ggatattctg gtataatagc acaggattca aactataaat
    19921 ttatgagaat aggtcttaat tgtgtaagtt ttgacagtag tcatacaatg ataatgatgt
    19981 cttccataat ggaagaagga gatttaataa ttgcaatttc acattctggt gaaacagagg
    20041 agattataaa aactgtaaag cttgcaagag ccaataatgc taaaattata tcaattactg
    20101 aaaataaaaa ttctgaatta aaggatattt cagatgtaca cttatcttat gtatcgggag
    20161 aaacagtact ggaaaccggt tcaatatctt ctaaattagc acaatttttc attattgatt
    20221 tagtatatac acaggtagta aaagaacttt ctaatgaagc tattgaaagg aaaattaaaa
    20281 ctacaaatgc tataaaattg tttaaaaatg aataaaataa gaaatttgta atgttttata
    20341 gagctctctt aaaaatttag gaatggagag atattacatg tctaaattaa aagattataa
    20401 tattgataaa gtaataattt ttaataattt aagtactaaa acgaagaatc aacttaaaga
    20461 aaaagttaaa ctaaaaagat tacaaaaaaa agaaatttta ttttatgaaa aagatagctt
    20521 agacagtgtt tatatacttt tagaaggaaa ggtatctatt tttaagataa gtgaaaatgg
    20581 agagagaaaa gtaattttca tattaaatag tggggaagtc ataaatgata ttacacttga
    20641 cgcttctaaa aactctactg ttggctgtga agcatttgaa aattcaattg tagcttatta
    20701 tagtattgaa gaatttttag aaattatgca aaatgatttt caacttacaa aggatattat
    20761 ttcttatatg gaaagaagaa taagacgttt atatagacag ttgaaaaatt ctatatctat
    20821 aaaagtagat aaaaaacttg cagcaaaatt atataggtta agtagagaat ttggagtttg
    20881 ctgtggagaa tggactttac taaatgtaaa tattacagtt acatatttag ctgatatgct
    20941 tggctgtaaa agggagactg tttccagaat gataaaggta ttgcaaaaag aagaacttat
    21001 taaaattgac aataaaggtt tttatgttaa agaaactgaa ttatcaaggt attttaaaaa
    21061 taattaatta tgattaagta agagtattat tagaatttta attaaataaa aatattatta
    21121 agagaatcta tttatagatt cttttttatt gtctaattta ttaattttta ttttacccta
    21181 ttgaattgta taaaaaaaca aataagattc ttttttattc aaaaaactta ctaaattttt
    21241 gattatcttt tcataagttg gtaaatgccg atatatcccg tatttgaggg ctttatcggt
    21301 attttctttt tggaagatac cttgcataag ctggcattta ccagcatgaa aatgcaacca
    21361 aaagtagtaa atgagtagta aatatacgtt ccgatattaa caagccagtc tttccagttc
    21421 tgcttttgca gattggaatg taccgtgtgc gtagtagcca agcgtcatgg ttatgttagc
    21481 gtgtcccatg agatattgca gggtgttcgg gttcattccc ttgtttgcca tattcgtaca
    21541 ataggtatgt cggaatgaat gtggtgtaat gtttggtaac ttgtctttgt ggtatttgtt
    21601 atacttctta atcagtcctc gtaccatacc cacatagttt ccagcgactt taggcaatcc
    21661 ctcacggttt aagaatagga aattgctgta accaccaaca accagcggtt gagcttttac
    21721 tctgttcttt agtattcttt ggagtgcttg ataagccctt tccgtcaatg gaatttttcg
    21781 ttttccgttc tttgtttttg gagtgtcaat atagtagccg atttcgctat ctcttaatag
    21841 ctggtggtct atattgagta ttctgttctg catatcaata tgcgttgtca gtccgcaaaa
    21901 ttcagaaata cgaagtcccg tttccagaag aagcacaacc tcatcataat acttgctgta
    21961 aatcttatcc gtttccataa aggcaagcag gcgttcttcc tgctctggcg taagaataac
    22021 tttcggctcg gtatcatctt ccagaacatc acttagctta aattcaaacg ggttctttct
    22081 gatacagtcg tcttgtatcg ccatataaaa tgacgctttc aaggaacgct tatagttgtc
    22141 aatcgttttg taggcatatc ctctttcact cattctgata gcccactctt tagcgtccga
    22201 ctgtttgacg gtatcaatcg ctctcatacc caatgggtca tttttcagag cgttcataag
    22261 atactgtcgt cctttttccg tagctcgctt gatgttcttt ctttggctgt tcttcttgtc
    22321 gtaaagctgg cagactgtca ttttaccgcc tgccgtgtca ataccgtcat caaggtcttt
    22381 ttttatctgt gcttcttttt ccctcaacga tatatcatca cgttttccag caggcgtttt
    22441 gtctgtcggt acaagtttcc aagcataaat aaactgtggc tttccgtata tatcggtata
    22501 tttataaacg tatcttccgt cttttcgctg gctctctcca ttacgcaaat tgcgattttt
    22561 actgtctttt cgtttcacat tagacatagt gtaaagctcc tttccgtcat ggaatgagcc
    22621 gtgatacgct atacttatta taccatatcc acggctctat gacattagat ttcgtccaat
    22681 gtatctatga ttttttcaaa ttgttttcgc tttatctgaa tacggttgcc gttcacgata
    22741 acccagccag cgtcaatatt ttcttcggct aatttacgca acttcttttc tccaatgcgg
    22801 aagtatttgg acgcttcttc aatcgtcagt gtatattttt cccagatagg cacatcagta
    22861 ttattcataa tttatgcacc ccctttactg tgtgtcagtc tataagttat ataaacaggc
    22921 agacggcttt ggaaagctcc attagtattt caactattcc cattcttgtg ggcagaaccg
    22981 caccatgcgg aagtatcatt atgctgtaag ggtaggtcat ggcaaaacga cttttccaac
    23041 ggtcgctctc ggatcactgc gtgggtcgct cgctttcttt tggaaaggtc atggcgtaca
    23101 gttcccgtgg ctctctatcc ttacaaacgt atctgtctgc tttattcagt tttcaaagag
    23161 ctgtggcgaa agcggtatgc tttcatgtaa aagcaggagc agagcaggaa agaggggatt
    23221 gaacaaatca tgctctgcgt tactgctggt tattcttctt caatttctaa ggcaatatca
    23281 aaccctaaaa tcattttgat aagtgcttct cggataagtc cttttaactc catatctaca
    23341 acgatataaa cattaccgta ttcatcatag agtggacgca aacagcactt cgatatgtaa
    23401 gggtcataaa atgcaagtac cttttcaatc gctttttcgt ccccgtccat agcttgtgaa
    23461 agcaagtagt aagacggctt cttaaatgtg tgtttcattt gctatcgttt ctcctttaat
    23521 agttttctca taagtgccaa tgaattgtgg cggttctgaa aaactccgct tctggaaatg
    23581 ttttgctttt tggcgatttc ttcattgttc atttccaaaa agtaatacat caacaaagtt
    23641 tctcggttac gttcggataa ctgtttcaag gcttctgcca attcatcatc atagacacga
    23701 atatcattac cgcatacatt gaaaatcgta tagtctgttt cgtagtcgtc ccatacagcc
    23761 aagttttcaa ccatgatttc tggaagttca caaaaggaaa tttctttttc ttttcttcgc
    23821 ttcaatccct gctgatagtc cttgacaaca tgacggacaa ctttcttcaa acagctttca
    23881 aaacggcatt gaacagtttt ctggaagtca gacggtttca tcaatctcac cccctttccg
    23941 ttatgacgtg aaaagtgttt atccctcttt ccgcaccata tagggaatgg caagtgtgat
    24001 ttgttaagtt gaaaccagaa aaaatcgaaa aaacttttta gtaaaacaaa aacagcccgt
    24061 gtgaatgtga atacacaaac ggactgcttt ataaatgcta tctgtatgaa tttttaactc
    24121 ttgtagaggg tagaatgatt gtaatactga actaaaatat tttttcagtt gggaacagca
    24181 aagtattatt ccatatacaa tcatggcgta gcccccttta ccagagctat accttgtgcc
    24241 accaatgaaa aaggacggct ctggctatat ccaaaaccgc cccgttatgt tttcaagacg
    24301 cacaaaagta tatcatctcg gaacggttat ggtgatttaa aatgagaaaa acaaaagaaa
    24361 cacatacttt tgatttcaga ccgctagggc tggcaatcag agaagcccgt gaaaaagctg
    24421 ggctttctcg taatgattta ggcgataagg tattctatgg ggaa
//
LOCUS       DAEMGG010000052        24279 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEMGG010000052 DAEMGG010000000
VERSION     DAEMGG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.052"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(115..795)
                     /locus_tag="KPK24_001957"
     CDS             complement(115..795)
                     /locus_tag="KPK24_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2299629.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVGLIDDFEVVLDEFIDR
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(947..2854)
                     /locus_tag="KPK24_001958"
     CDS             complement(947..2854)
                     /locus_tag="KPK24_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2299630.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKITLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNVYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(2855..3607)
                     /locus_tag="KPK24_001959"
     CDS             complement(2855..3607)
                     /locus_tag="KPK24_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299631.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(3923..5077)
                     /locus_tag="KPK24_001960"
     CDS             complement(3923..5077)
                     /locus_tag="KPK24_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2299632.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(5080..5547)
                     /locus_tag="KPK24_001961"
     CDS             complement(5080..5547)
                     /locus_tag="KPK24_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2299633.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(5561..6049)
                     /locus_tag="KPK24_001962"
     CDS             complement(5561..6049)
                     /locus_tag="KPK24_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299634.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSSF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(6063..7394)
                     /locus_tag="KPK24_001963"
     CDS             complement(6063..7394)
                     /locus_tag="KPK24_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2299635.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     DRQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(7408..8973)
                     /locus_tag="KPK24_001964"
     CDS             complement(7408..8973)
                     /locus_tag="KPK24_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2299636.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(9143..9928)
                     /locus_tag="KPK24_001965"
     CDS             complement(9143..9928)
                     /locus_tag="KPK24_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2299637.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(10095..11294)
                     /locus_tag="KPK24_001966"
     CDS             complement(10095..11294)
                     /locus_tag="KPK24_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG2299638.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEEMSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYIDTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKQYTNARNIKLNPVENLPTSVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(11816..13153)
                     /gene="xylA"
                     /locus_tag="KPK24_001967"
     CDS             complement(11816..13153)
                     /gene="xylA"
                     /locus_tag="KPK24_001967"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG2299639.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(13249..14799)
                     /gene="xylB"
                     /locus_tag="KPK24_001968"
     CDS             complement(13249..14799)
                     /gene="xylB"
                     /locus_tag="KPK24_001968"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG2299640.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            15007..16167
                     /locus_tag="KPK24_001969"
     CDS             15007..16167
                     /locus_tag="KPK24_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG2299641.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(16323..16727)
                     /locus_tag="KPK24_001970"
     CDS             complement(16323..16727)
                     /locus_tag="KPK24_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2299642.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(16748..17230)
                     /locus_tag="KPK24_001971"
     CDS             complement(16748..17230)
                     /locus_tag="KPK24_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2299643.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(17250..18059)
                     /locus_tag="KPK24_001972"
     CDS             complement(17250..18059)
                     /locus_tag="KPK24_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2299644.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(18052..18831)
                     /locus_tag="KPK24_001973"
     CDS             complement(18052..18831)
                     /locus_tag="KPK24_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2299645.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(18831..20909)
                     /locus_tag="KPK24_001974"
     CDS             complement(18831..20909)
                     /locus_tag="KPK24_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBG2299646.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(21708..22229)
                     /locus_tag="KPK24_001975"
     CDS             complement(21708..22229)
                     /locus_tag="KPK24_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG2299647.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            22395..22961
                     /locus_tag="KPK24_001976"
     CDS             22395..22961
                     /locus_tag="KPK24_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2299648.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTIGTVLGAVV
                     LGPFWSALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(23178..>24279)
                     /locus_tag="KPK24_001977"
     CDS             complement(23178..>24279)
                     /locus_tag="KPK24_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBG2299649.1"
                     /translation="FVSYPFILELFDDYNEGIINKKVLVESLNLVECFILRRIVCDVP
                     PNALNKVFMNLGREIKKFSDYKENYSNILRYIFINKKSSQRFPTDSEFSNAFETKDIY
                     NTKSKNKLYILERLENYNNKEIVDLENLINNNMLNIEHIMPQTLTNKWKESLGDNYKE
                     IHDKYLHTIGNLTLTGYNSKLSNKTFEEKKEMEDGFKDSRLYLNKYISSIDKWNEEEI
                     KNRTKILLDRAVEIWKYPSVTYEPPSNSENIFTLEDDDVNFTNESIVSFSILGESYKA
                     KNWTEFYEQVALTLYDLDSVRFNSIVDKTYKRDFIDNIKSRDAYKLRKGIKISENIYL
                     ETNLNTEAKLNIIRFLLNEFDIELNEVIFSIK"
BASE COUNT         8092 a         4188 c         2545 g         9454 t
ORIGIN      
        1 ttaactctga atatttattt ttaaaaatat ctatagcaaa taagtagata tttattatac
       61 taactacaac taaaggtaga gcaatatact tagacctacc ttttaaatat tttactagca
      121 ttgtattata tatcctatcc ccttcttagt ttttataaaa tcatcaaaac ctagttcttt
      181 aactttactt ctcaatctat tcatatttac agtaagtgca ttgtctgtca caaactcatc
      241 attatcccat agagtcatca taagactttc ccttgataca accctattct tattttttaa
      301 taataatgtt aaaattttta tttcgttttt agtcaattct atacttttat ccttatactt
      361 atgttttata atccctttcc ccacatcaaa catcaaatct tcacaataca aaatactatt
      421 agaatcacta taattataga ctcttcttat tataccattt attttggtaa ccaatatttc
      481 tatagaaaat ggtttggtaa tgtaatcatc tgctccatta tttataccca ttattaaatc
      541 catatcagaa tctcttgatg ataaaaatat taatggtaca tttgaaatag ctcttatttt
      601 ttcgcaccag taaaatccat tatataatgg taaatttaca tctaaaagta ctacatctgg
      661 ttttctatct ataaattcat ctaatactac ctcaaaatca tcaattaatc ctactttaaa
      721 tccccatttt tcaagtgcta atgcaatctc ttttgataaa tcaatatcgt cctctactaa
      781 taaaattcta tacatttgta tattctccat tatcctaata tttcactatg ctatgaatta
      841 ttaatatcat aacatatttt atatgaattt attacccaaa ttatttagtt aatcaacaag
      901 gagactctta ttacaagaat ctcctttctc tttattaata tatcaactaa tttttataca
      961 ctacactctt aaacttatta acagatactg ctccatatat tatgaatatt atcaaacata
     1021 tcactaaact tagtatatat ataccaaata aatcggtcat caataaatct cctgctaatc
     1081 tcaatgcaaa ataactagat atagttgcac ttaacattgg caaagtataa aaaatagcta
     1141 cttctttaag tatagctcta tttatatctt tttctgtagt acctattttt aacaaaatct
     1201 tatatttctc tttatcttca tatgcatcac tatatatttt catatatatt atacttccag
     1261 ctacaattgc aaagactaag aataaaaaca caagcactac atatgcaaac ttcatccatt
     1321 gactctcacc ttgagcttta tatccatttt gaatatacac attttttaaa cttaaattct
     1381 tttcaatatt gttgaacatt acttgactat tttcttcttg aaataccttt gcaccataaa
     1441 aatacacttg ttgaccctta tctcttagtt taaaatatgt atcatcattt accactataa
     1501 tactttgatt aagtcctgtc cctagtactg gaacttttac ttctcccctt gaaatattat
     1561 ataactgacc atcaagtttt atcttggctt cttttgtagc tattgatgct atcgtttgag
     1621 ggctttttaa ctctattaca ttatttccac taactaatct ttcaccatac tcttttaatt
     1681 ctttatcatt gccattcact ttaagagtat tacaaaaatc actatatttc attatagtat
     1741 ctgttgtatc ataagcttct tttatatctg ttttctttaa aattgttagt tctggattta
     1801 ctgagtactt tatcttatta gtatctttta ttatattctc tatttgtttt ttattcactt
     1861 catctttatc cattgaaact atgctaatag tgtacacatt tctttgttct tgggcattct
     1921 tatgtatacg cttcatcgca actgaagcac caagagttga tattgttgta gttataatta
     1981 tagctattat agcataagtt ttatagtttt tatttaatcg gtagtttata ttgtttattg
     2041 caattatatt atttccatta aataatattt ttttattttt gattataata tcaaatataa
     2101 caggcataac tgatttgaat aatccataag tacctactat tattatacct ataacaggta
     2161 gtacactagt tatatctcca gttcttatat tggtagaaag atagtagcca tatcctatta
     2221 ctataattga tactatagct aatatatata tacctattcc tacctttggt attttttctt
     2281 gttttttaga tatattgatt agatttatta cactacttct acatattgtt ataaaacctt
     2341 ttacagccat tattataaat atagaaataa agattaaagt agcatatagt atagcttcta
     2401 tactaagtcc attagatacc tttatattaa atccagataa ctttattatc actacttgaa
     2461 aaaatctaga tgttattatc cctataatta ttcctgttat acaagaactt attcctataa
     2521 gcatattttc tacaaaatat atttttccta tagtatataa atctaatccc ataaaagaca
     2581 atattccaat ttctttagac ctattcttaa aaaatatatt tgttgcatac catataaaga
     2641 aaaataaaaa tactgatagt atcattatta cagctactaa cactgatagt gtaatttttt
     2701 tattttcatc ttgtaacaca tttatagcat caccatatct taataattca aaattagtta
     2761 gcacaactac tgaaaaaacc atagatataa aaaatgctag ataagttctt atgttactct
     2821 taaaattatt ataagcaatt ccaagtatac tcacttacat ctcacctccc atagaggcta
     2881 gcatatccat aatttttcca taaaattctt ttctactatt tcctttgttt aatttacact
     2941 ttatatttcc atcatttatc atatatactt catttgaata acttgctgtt agagggtcat
     3001 gtgtaatcat tataatagta gcgttatttt ctctgtttat tttttctaat gattctaaaa
     3061 gttcatatgc tgatttagag tctaaagcac cagttggctc atcagcaaat atgactgttg
     3121 gatttgtaat taatgaccta gctgctgcgc ctctttgttt ttgtcctcct gataactgat
     3181 atggatattt atctaaatga ttttctaatc caaatttttt agctatagaa aataccttag
     3241 cttctatctc cttacttttc ttttttccta atgctagagg aagtgctata ttgtccatta
     3301 gtttcatatt gtctaataga ttatagtctt gaaatataaa ccctattttt tctcttctta
     3361 attttgatag ttcattattt tttacttttg atatattaat tccatctagt attatttctc
     3421 ctgaagtttg tttgtcaagt gtagacatta aatttagcaa tgttgtcttt cctgcaccag
     3481 atggacccat tattgatata aaatcacctt cattgactgt aagagatata ttttttaata
     3541 catttgtttt aaatcctttt attccataat cttttgatat attttttatt tttaatattt
     3601 ctttcataat aatagcctcc agttgctaat tttttaatat atatttattt taaaggtttt
     3661 atataaataa atccttaagt aaatattaca ttttaactgt tagcattaca cttttgtaac
     3721 atatggaaga ctattataat tataatggaa atatcttaaa ataaaagcat gatttacttc
     3781 tttgtataaa caaagaaaag aagctcctaa gaagcctcta atctatacaa ttattcttct
     3841 ctataaaaaa ccaaaataca cattgattgt ggcaaataca gatactataa acaagtgata
     3901 ttactactat atattaaaac aattattcct gaagataatt caataagact atcaagcaat
     3961 aagctaagca tccaaaaaga cttaatacca ttaatgtata attataccca atattaatgc
     4021 tcaaaaaacc agcaacagtt ggaataatca gagatgaacc tcccataaat atacttaaag
     4081 ctattaattg gctactactt ctaagttttt taggagaaac ttcaaatatt aatctttttg
     4141 aagtaattaa aattaaagga gtagaaaaca cttgaaaaac acacagtata ataatgatat
     4201 ttgaatcatt tgttaaagca aataatagaa actgaactgt tatcataata gcacttaatt
     4261 ttaataagtt taaactgcta tattttttta ataaataaga accagccaga tatgttggta
     4321 tctctaacaa tgactgtaaa gaccacttta aagatatctg agaatttgta gcatttaaag
     4381 ctatcatttt atctattaca gtacagttat ttgctaccac cattgagtat aataagaata
     4441 aaatacatac tagtaagata tattttttat cttttaataa ttcaataata tctttattag
     4501 ttacatttat tttcttatta atcttttcaa tatcaggtaa taatagtata tttataataa
     4561 gtaatactac aattaaacca atcgctacag ataatccaaa atatgagaat ctaagaatca
     4621 agtaagatgc taccatactt ccaatagccc atccaatcga cccaaaagtc ttaataaatg
     4681 acaaataatt attaatatta tctccacaac ctattatcca tgtatcatat aaactacagc
     4741 aagtatttaa taatcctcct gataaagcaa caagtaataa gtatattata aaagttgcag
     4801 attcagtaga aaacataata actgatgcaa ttccataaac tgataaacta attaaaacaa
     4861 tttttttcat tgtttgatac ttatcagcta atactccaaa taataattga aacaatatat
     4921 tggttattgc atagctcgat aatataattc ctctttcaaa cgctgagtaa cctaattttg
     4981 ataagaaagg tgtgaatttt gttgaagcaa aggcaaaaat aatatatgag aaaaaataga
     5041 atattataaa tttaaattta atcgctttat taatcataat caatcaaaat tgataacttt
     5101 aataccacta tttccttcct gatttaagtt tgaaagatga tattttaccg tattaggatt
     5161 ctctacaata actaaaggaa tatataaagt attttcaagt aacttattat caatttctac
     5221 taacttttcc cccatattaa cttcttgacc ttgtttaact tctaatttaa agttttcttt
     5281 ttttgttgtt gcactatcca atccaatatg aataagaatt tgaacatcat tttgcaattt
     5341 aattcctata gcatggttag tttgagcaat aaaacttatt actcctttag tcggtgattt
     5401 aagaatattg tcctttgcta tcatagcaaa tccatctccc attaccttag ttgaaaatac
     5461 tttatcagga atttctccaa gattaattat ttttccatca atgtatgaat taattgtaat
     5521 tgcttctttt tttcttttaa agaacattat agacactcct ctacataatt aactttattc
     5581 taattatcat tacaaataac gatattaagc atactgcaat tgaggaaaaa agagccttat
     5641 agctctttcc ccattgtagt tctttaatac tgcttatgat ttgtttaatt ccaacaaatt
     5701 tataaatata ggtaaacatg ataacaatag ttattgtgta aatttggaat atctgcccta
     5761 ataagactaa atagaaagca atcatgtttt cttttatctc tttattcttt tttatattgt
     5821 aaataacata tgcactgtat actgcactaa agttagagat taaatttaaa cttgcattaa
     5881 catcattgtt taaaagttgt gaaaatgaaa tatttgtaaa tgatgatgtc aattctaaat
     5941 acactatata taaaacaggt aatattagaa aacaaaccat aaccactgaa agaaatatat
     6001 tttcatcttt taatactgct gtgatatttt ttattttatt tgttatcatg tttttgttct
     6061 ccttatttaa aagttggcca gaaatcctta ttagccacaa tcatttcatc taagatttct
     6121 tttgctactt ttgcacttgg aacagtttta ctcatagtaa acgactgcca taatttttga
     6181 taactttttt ctatccatgc ttctacagct aatttttcag aagcaacttg ttgttccatt
     6241 aaaccttttt ggaaagttgg aattttacct atagacatag gttcaaccat atcttttcca
     6301 acaaggcaag gaatttcaac cattgcatca tctgcaaaat tttcaatagc tccattatta
     6361 ggaacaatca gtaaaaatct ttcctgagta ttaaatgcta atgcacaagc taattcaaca
     6421 ataaattgag aatgttcact tgcatcaaac catgtatctc ttgctgtgcc attttttata
     6481 attctttcac attcttcgaa tgtatccttt tctcttctat ccataatttc atttgctctt
     6541 gtataatttg gattagcatg ttcaacagtt tcatcagcca ttaaataata ttttaaataa
     6601 gtattaggta atgtagttgg ttctatttcc aataaatcct ttgctgctct catagtttct
     6661 aaccaactag attctttgtg ttgaccttga ggtgtaaatt cctcatatcc ttttttagca
     6721 caatattcct gtaatttagg cattaaatca tttccttctt tgtccttaat acttgtccac
     6781 cacccgaaat gattcaatcc ataatatcta acatcaaagt ctttacgaga atttaaacct
     6841 aaaactctag caaatatttc ctctaaacat actggcatat cacaaatatt aataattctt
     6901 gaatttggtc ttaatacacg acatgcttct gcaactatag ctgctggatt tgaataatta
     6961 agcatccaag catttggaga atatttttcc atataatcaa tcatttcgat tattggacct
     7021 attgttctta atccataggc aattccacct gccccacaag tttcttgacc aacaacccca
     7081 tattttagtg gaattttttc atcttgttca cgtaaagcat ataaaccaac acgaatttgt
     7141 gccattacaa aatcaatatc agtaaatgct tcttctggat ttgttgtata actaaattta
     7201 atttctggat ttttctctct aacaattatt gcacatgctt cagcaacttt tctttgtcta
     7261 tcatcattat tatcatataa tttaattgat tcaataggaa atttagcaac actttcaatt
     7321 aacattgtta caattccagc agtataagta ctacccccac cagcaattag tatcctctgt
     7381 ctttttcttt ccatctttcc ttctccttta taataaattt tcaaattctt ctcttacact
     7441 tggtactgaa agcccaataa taatctgaat agcttttcca ttatgcacta aaccatgtgc
     7501 acctactgcc ttaaaatctt cttctttaga aataattgag tcatctttca aggttaatct
     7561 caacctcgta gcacagtttg taacatctac aacattttct gctccaccaa ctaattttat
     7621 gtattcagat gcttgttgag aggctttact caatttattt cctttttcat ctttagtttc
     7681 taagtattca tttttagtat atagttttgt ttcttcacta tcttcctctc taccaggagt
     7741 tttcatatca aacttcttaa ttaagaaagt aaatacaaag aaccagataa atgaaaatat
     7801 aatccctaca acaacttgaa caacatatac cttccagtga tacattccaa gaggaagcca
     7861 atttaataca acccagttaa taattcctcc accaaaatca ccaacaactc ctagggcaaa
     7921 cgaaatcgtt tgcaagcatg catctaaaaa tgcatgtact aggaataaaa gtggtgctac
     7981 aaataaaaat gtaaattcta acggctcagt aattccagtt aatactgcag taagagtagc
     8041 aggaatcatt aaacctaata cttttttctt tttttctggc tttgcagtta catagaaagc
     8101 accaaataca cctaatgaac caaacacctt cgataatccg gataatgaaa atccgattgg
     8161 atacatttcc tttagagtct tagctgaagt ttgaaaatcc tgaatatgag tagcccaata
     8221 tactgatgta ccacctggaa caacaacact atcataacaa attggtgcat atacaaaatg
     8281 atgtaatcca gttggtatta aaatcttttg taagaatgca taacaccaaa ctcctatatt
     8341 tccactattt ataaaaaacg tttgcaaatg tctaataaca ttttgaatat gaggccaaat
     8401 aaaacaagtt aataatgcaa ctggtatcat aacaaagaaa caaattgcaa ctacaagtga
     8461 tgagcctcta aataatccaa taaaatctgg cagttcttta tcataaaatc tattatgaat
     8521 ataaatgaca atacctgata ttaacaaagc accaaccata ccagtatcta atgttttaat
     8581 accagcaatg cttacaagtc cacttccagc acttatttca gctgcgaaat caactccaaa
     8641 gctttttccc caaaaactta acatagaacc tacaaaataa ttaaaagtca aatacgttgc
     8701 taaagcctcc atacaagctc ttgcttgttg cttttttgca agtgctattg gtaaagaaat
     8761 agcaaataat aatggcaatt gattaaatac catccaagct ccactagata ttacacccca
     8821 aaattggtac caaatatttt ctggtgtagc aagtgaaccc ataattgact gattttgaaa
     8881 tacaattgag aatccaacca ttatcccagc aaaagcaaat aatagtgttg gtgtaaacat
     8941 tgcccctcca aaacgctgta ttttcttcat catataaatt actcccctcc tcatattttt
     9001 agtttatctt tggccttaga tatctattat tttaacaggt taataaatat atacaatctt
     9061 tagggtacat tcggagctta ttcacagtca aataagtttt tgcacatttt tatgttcaaa
     9121 ttctcataaa aaaaaagaat gactacaatt tgcaatcaat cccttctgta atcattctat
     9181 gccttttata aatactatat ttaataaata ataactcaat caatataaaa tataacattg
     9241 tcaatttata actagggtgc atttgtaaaa atgagatatt agtcgctgaa atataaattg
     9301 attcatcact taaatcagtc aatgtattat ttttaaactc tgtaattgaa attattttag
     9361 ttcctattaa ttttaatctt ctagcaatat taagtgtatt ctcattctca ccacttaggg
     9421 atataataat cataacatca tttttactga caatattgta aaaagaatca tcaaatgttc
     9481 ttccctcaaa atcatatatt atttcttgac atgataagaa cattctcttt atctgttttg
     9541 caactacatt ttgaatatca cctgaaccat aagcgtatat tgttcttgat tcataaagaa
     9601 gtttacacaa tgcatcataa tctttatttt tttgatcttc aacatactta attattgaat
     9661 tacatgcaat atcaaatgac tgattttcta tgacatgaac cttttctgat tcccagctta
     9721 ataatacttt aaactcactg aaacctttta aatctaattt tttaacaaat cttgtaatag
     9781 ttgcacgaga cacattacat ttcatagcaa gttcatttat tgacatctta ctacaatgtt
     9841 ttttatttgc agttatatat ttccatatta ataaatctgt atcattcaat ttattataat
     9901 ttaaaaaaat catttcttct aatctcataa actattaccc cctttaatta aaaatcgtca
     9961 taaatattat accaatattc aacattaaat tacagcgtat tcttatattt actatttagc
    10021 ccataaaatt gactataaaa aatactgcta taattgatgt ttaaaaaaat gtagttttaa
    10081 ttttattctt ttatctaata attaatattt tcaagttcta gtgcttttct tatacaatta
    10141 tcatctttaa aatcagcagt ccttttacaa tcttttactt taaattctgg aatagtttta
    10201 aattcctcat cacatattcc attcttattt aaatacatac aacttgccat tctaactatt
    10261 agaccactat tttttaaatt aaacaataca gggtctatac accctccatc tccgccagta
    10321 gtttcaccaa ctaaagtcgc aaatttagtt tctttacaaa acattgcaaa tgactctgat
    10381 gatgaatata tttttctatc aacaagtaag tatatattac ccttaaattt tgaatcatct
    10441 gtagatttta tagtatagga agtatcttca aaatcattaa aatcttttat aatttcttta
    10501 ggtgcatttt cctttacact tgttggtaaa ttttctacag gatttaattt tatatttctt
    10561 gcatttgtat attgttttac tatttcatta ttatttctat aaattctata accatttatt
    10621 ttgacatcat tttttatgag tttagatacg attccctgcc aataactatc acttccacct
    10681 aaattacctc ttatatcaat aattaaagct ttatgttttt ctaaagtgtc aatataatct
    10741 ccaattattt ttaaatcatt atttatagag ccattctttg atgacattga tggtaaatac
    10801 atatatccaa tctgaccctt aataacatcc tttaaaatta attctttttt tacaaaaata
    10861 tcatctggca tttttatttt aagattcata gagtcatatc tatctacaac ttttttatca
    10921 ttaagaaaat cataccaatc attattagag tatgatttct taaaaagttc atatcttttc
    10981 ttattatcta ttacttctgt gtgtttatta tttaaatcag ataatataga tgacatttct
    11041 tctataaatt cttcatcatt ttcagtgttt tttattcgtt ttgtatattc atccttattt
    11101 gctaaccaat caatattatt aagcctctta tttacaccta gatatggata tcctgtcttt
    11161 acaacattat acacataatt aaagtcatcc accttttcct cttcagacaa ctgattcttt
    11221 tgagaataag tagaattacc taataagatt ataaatgcta aaataattac taaaaataat
    11281 actttctttt tcatgtaatt ctcatccact ttcagatttt atattttaat acattgtatc
    11341 attattttat tatcacctaa acagttaaaa gaaattcttg aagatatatt ccactattat
    11401 tctctttaaa aatttttatt tgtattttca aaattatttc atgaaagtta taaaataaca
    11461 attatattca aatatctatt ttactgctat atcctgaagg taaattctta tcagtgatat
    11521 actaaaaaag atatgctaaa aaatttatcc attataatga cttaaattta gatactttac
    11581 tgatttttat ttattagttg agaaagatat tgataattaa aaatattgat aactatagaa
    11641 atgattattt tttatagaaa ttttaaggat ataaactaat tatacttaag tctatatcct
    11701 taatttatca tgattatgag aatttttaca aatacttatc tttgtccgtt ttagaataaa
    11761 aacaagaaca tatcctgtta ttttattcaa acatccaaat ttaattacta cacaattact
    11821 tattagaaat tgtatctact tcatatatat atctgttaag tatagcttct agcatttctt
    11881 gtcttccaga cttattcttt ataactggca tttgcagtgc ataagattct aactctttaa
    11941 atccaacctt accttctact atatccttac ctataccttc actaaaactt gcatatcttt
    12001 ctttagtaaa gttttcaaat acctcatctt ctaataattt atgagctatt aataatccct
    12061 ttgcaaatgt atccattcct gcaatataag ctaggaataa gtcttctacc tcaaaagatg
    12121 ctcttcttac tttagagtca aagttaaatc ctcctggtgc tattccacct tgctttaaca
    12181 cttcatacat agcaagtgtt gcatcatata tatttgttgg aaattggtct gtatcccaac
    12241 ctaataatag gtctccttga tttgcatcta tactacctaa aacattgttt attcttgcta
    12301 tatttaattc atgttggaaa gtatgtcctg caagtgttgc atggtttgct tctatattca
    12361 ttttgaagta tttatccaga ttatactttc ttaaaaatcc taaaacagta gcagtatcaa
    12421 aatcgtattg atgtttagta ggctcttttg gcttaggctc tattaagaat tggccagtga
    12481 atcctatttc cttagcataa tctacagcca tttgtaagaa tcttgcaaag ttatccattt
    12541 caagttctgt attagtgtta agtaaagttt cataaccttc tcttccaccc cagaatacaa
    12601 aattttctcc tcctaagtac ttagttattt ccatagcttt tttaacctga gctgctgcat
    12661 atgcatatac atctgcatta catgttgtag atgcaccatg aacaaatctt ggatttccaa
    12721 acatattggc agttccccat aataacttta tacctgtctt ttccatatta acttttatat
    12781 actcaactat ttcatctaat atcttatttg tctcttctaa atctcttcct tctggtgcta
    12841 tatctctgtc atgaaaacag aaatactcaa ttcccatctt agacataaat tcaaatcctg
    12901 catcaacttt aatctttgct atctcaattg ggtctgttat attattccaa ggtctctcta
    12961 ctgtgcctac tccaaatggg tcattaccct ctccacataa agtatgccaa tagctcattg
    13021 caaatcttaa gtgttctttc atagtcttat tacctaccac ttgttcagga ttgtagtatc
    13081 taaatgctaa ttcattgtca gattttactc cttcaaattt tatctgtcct attcctttaa
    13141 atatttcact catttcatgt cctcctaata ttttacacat ataatatatg tagtatgttt
    13201 tcttaattta aaactatttt ctatctttat ttttcttttt aagctaattt aattatttat
    13261 ttttgcatta actacttcat ctaataactt aaagtcatct ttcaaagatt tgtatattct
    13321 tttatacaat ctatgatata tattgtatgt atctacattt tcacttattg gttctaattt
    13381 agttgaagtt tttattatag catcacatgc tttttgtata ttttcatata ttccagctcc
    13441 tactaatgca agaattgcaa ctccaagtgc tggtccttca cttgaattta tagtatatac
    13501 atcttgatta aacatatctg cttgcatctg tctccatact ttactttttc cacctccacc
    13561 tgaggctctt acttcattaa cgtctatatt aagattctct attatatcca tacagtcttt
    13621 aagactatat gaaacacctt ccattattga ccttgcaatt tcttttttag aatggattgg
    13681 agataatcca aagaacacac ctcttgcata agggtcaaga tgaggagttc tttctcccat
    13741 catatatggt aagtaaagca atccattaca tcctgttggt acttgactta cttgattatt
    13801 tataatctca tatacatctt cacctaaaga atttgaaact tccacttcac tttgacagaa
    13861 attatcttta aaccatttta atgacaagcc agctccttga gtaactccca taacatgcca
    13921 tgtatttggt attgcatgac agaaagtatg aactctacct tctttatcaa tttttggctc
    13981 ttttgtataa gcaaatacta cacctgatgt acctatagta gatgaaacta ctccatcttt
    14041 aactattcca tttcctatag ctcctgctgc ttggtctcca gctcctccta ccactattgt
    14101 tccttcttta agccctgtca aacttgctac atctctagtc acataacctg taatctcttg
    14161 tgattcataa actttaccta aaagattttt atctatattt aacttttcta gtacttcatc
    14221 gctccaatct ctttttctta tatcgagcaa ctgcatacca cttgcatctg atacctctgt
    14281 agcatattca cctgtcaatt tgtatcttat ataatctttt ggaagaagta ttttattagt
    14341 atttttatat atatctggtt cattgtttct aacccataat atctttgatg ctgtaaatcc
    14401 tgtaagtgct ggattagcag ttatttcaat cagtctttct tcacctacta aatcagttat
    14461 ctcctcacac tcttttactg ttcgttggtc acaccatatt atagagtttc tcaatgtttt
    14521 accttcattg tccagcataa ctagaccatg catctgtcct gataatccaa ttccttttat
    14581 acattcactg cttatatttg aactttgaac tacttctctt atagattcga ctacagcttt
    14641 ccaccaatca tttgcatcct gctctgccca acctgatttc tcttgtataa gaggatattc
    14701 atatgtacaa ctttttatag tatttcctag tttatcaaat aaaactgttt tagtacctga
    14761 tgtaccaata tccacaccta atacatactc caacttcatc cctccttgaa tgccacttca
    14821 taactatcgt tttcctatcc taattttttt atattttagg tatattttac ttgctttatt
    14881 tttgttagta tattaaacta acttatgaac taataataaa atatttaatt atctttgtca
    14941 atgcaaaata accttttatt tttgtgtaaa aagtattaca atattatttt aaggaggaat
    15001 atcaaattgg ttactgataa atacactatt cgagaaatga atgaaagatt ggtacttgaa
    15061 caaattataa aaaatggtcc tatttctagg gcaagtatag cttctactat aggattgaat
    15121 aaagcaacta tatccgctat taccaaaaaa cttattgatg aatctctagt tcatgaaatc
    15181 ggaattggaa atagtactca tagtggcgga agaaaaccca tattattagt atttaataaa
    15241 tgtgcaggga tttctttaag tatggacatt ggatacgact atatattttc ttctctatct
    15301 tacttagatg gaactattat aaattctaag aaattaacag atatacaagt aagtaaagac
    15361 aatgttatac aattgataga tgaaattata aatagttata acatatccaa aatagacact
    15421 ccttataagg taattggtct tactttggct atacatggga ttacttgtga aaataaagta
    15481 ttgtttactc catattacaa tcttaatgag attgacttat atagcatcct tagcaaaaag
    15541 tatgactttc ctattcatat agaaaatgaa gcaaacttaa cagctttagc tgagaatact
    15601 ttttcaactg tacataactc actacttagt ctcagtattc acagtggatt tggttcaggc
    15661 attataataa ataataaatt atatagtggc agaaatggta tgagtggaga aattggtcac
    15721 actatcatca tgcctaacgg taaactatgt ccttgtggaa atcgtggctg tttagaacag
    15781 tattgttcag aaaaaaaggt atttgaacag ctttcttctt tagaaaatat tcctaaaata
    15841 gattctgata tagtaaagca actatattat gaagataacc ataatgccaa aaaagttatt
    15901 catgagtttt gtagttactt aacaatagct ataaataatg ctattacaac ttatgctcct
    15961 gaaataatat atttaaatag ccaaataatt agtgatatac ctgagatatt acagattaca
    16021 aaagatatgt tagttagctc ttttaacaaa ggaataaata tagaaatttc atcattaggg
    16081 tctgaggcat ctctatatgg tggctctgct gtaaatatca agagcttttt gaatatacaa
    16141 aatttaactt taattaatga aatataaggg ttatgactat atacagccat aaccctttaa
    16201 attatacatt ctaaaattag atttattata tagtttatgc aagattagct atatttttta
    16261 aaaattgtat atcaattaag tattaacata agtacttcgt tttaaatatt aatataatct
    16321 tcctactcat catcagataa taaacttgca ttttgttcaa ccatagaaat attatcttta
    16381 cctattttct ctaattttgc tgccaattcc tttgtatcta aaaaagctga ttcaataacc
    16441 atacctaaat ttagccctga aataatttta attcgactgt cattattggc tactaataaa
    16501 gcagcattgc atggagttcc acctagtaaa tctgccatta taattatttc tgaactttcc
    16561 ttgtacactt ctaaagcttt ttttaatttt tctattgttc cttctattcc atcatcttgt
    16621 ttcatattta cagtaggata atctatagaa tcccctatta tcatttttgc agaatttaag
    16681 acttcttcag ccatattgcc atgactcatc aaaataatac tattcaattt aatacacctc
    16741 tttaaatcta taagatgtct acaagataag ttttcttatc atcaggcgtc attatagttg
    16801 taatttcaat tccttcatct aaaagtcttt taaatgcttc tttctcttct tcagtaacac
    16861 tgatattagg tcttagaact gttgtatctt ttctacttga catatttcct acatttatat
    16921 tatgtatttt aaatcctgcc tctacaaatg gaataaggtc cacaggagac ttaacaatta
    16981 ataatacatt ttgtccttca tactttcctg cagtaatatt ttttatagca gtctcttctg
    17041 taattataga tgacgctata cctgcaggtg tagccattct taaaacttgt ttttgaacag
    17101 aattgttagc tactgcatca tttactacca ttaatcttgt aacacccaat ttattactcc
    17161 acatagttgc aacttgacca tggattaaac ggtcatctat acgaatattt ttaatacctt
    17221 tcatttccat atctaatatc atcctttcct tattttaaaa tacctaatgc atataaaatt
    17281 attgaaacaa ccattactcc taaaattaag tatataggtt ttatattctt tttaagagcc
    17341 caataaacaa tcacaactag aattgcagga actaacccag gcataatttg gtctaatatt
    17401 tcttgacctt tcattgtaaa atcaccttgt tgaaagttca aagcaacatt agcttttaca
    17461 actgaaggta ttaatgcacc tacaaccatt aaacctaaaa tagatgtact ctcagttaaa
    17521 atctttaaac ttttttcaat tgaagatatt agcttagtac ctgatgtata accagcatta
    17581 aatagtggta tacttaataa tttcaatgct acactcacta caatccacag taagattcca
    17641 aatggacttc catctatcgc catattagct gctatagctc caaaaattgt ccacggaata
    17701 acgacaaata aactatctcc taaacctgct actggtccca tcaagcctgt tttaattgaa
    17761 gcaacagcct cttcagtctc ttcacttgat aactcctcca ttgctacatt aatacccaaa
    17821 atataagggg caacccaagg attagtgtta aaaaattgta aatgatttaa tacagcactt
    17881 tgtaactttt ctggattatc tttgtatact acttttaaaa aaggtagtac tgcataacag
    17941 tatgccaagg cctgcatctt ctcataattt gaagaaacag aactagtaaa aaaccagcgc
    18001 cataagacac tttttttcac cttttcattt gatttattta atgatacctt atcactcatc
    18061 ttcgtccata cctcctacta cagtatattg ttgttcttga ttttccaaat tctttttgta
    18121 aattataagt gcaattgctc caccaattaa agctacacct aataatggta ctttcatata
    18181 tactgctaaa acaaaaccaa ttactaagta actaaaatac tccttagcag gaagatatct
    18241 aagtaataat cctatcccta ctgctggaag tagtcctcca gctactgtta aaccacctgt
    18301 taaccattca ggtgtatatt tcaaaatggt attaattaaa gaaggtccaa atattacaga
    18361 tagagcaact ggaatacctg ttgtagctgc tgttaatagt actcctaaca tttgcatata
    18421 ggttatattt ttataatttc cttttttagc tccatcttct gctttatgaa ctagccatat
    18481 accaatagta tttctaagaa catctaattg taccatcaat aaagcaactg gaatacctat
    18541 agagattcct acagatgctt cttgcttagt cgtaatagtt ataaatgtgg ctactatact
    18601 tgctgtttga taatcaggta ttgatgctcc accaaagtta ctaataccta atgataatag
    18661 ctgtaatgta cctccaatat ataaccctgt atttatatct cctaagataa gaccagtaat
    18721 aaaacctgct gttactggtt gatatgttcc aaacattgtt gaattttttt catagtttat
    18781 aaaaaatcca tatagtacga ttaataataa ttgccataat tgtggctcca ttatgatttc
    18841 acctctccca aaatattttt tagttcttta aaattataac tggaatcttg ctttaggaaa
    18901 ataggaattt cttccagtgg gcattcaact tcataagttt tcccaccttc ataactaact
    18961 ccactaaata tattttccca tgtatgtcct tttggtaaat atacttttct acttctatct
    19021 ttataattca ttattggagc taccaaaatt tcatctccaa gcatatatgt attatccacc
    19081 tcccaagttt tatcatcttc aggaaattca taaaataaag ttctcataac tggtgttcca
    19141 tcttcatgcg cttctttcat cagttttttt aaataagttt ttaagctctc tctaattttt
    19201 atgaattttg taagaattac ttcaacttct tcaccataag accatatttc attaggagct
    19261 ccagttacca ttgagcctcc acctttattt gataatggtt tactatgagg caatctatct
    19321 ccatgcattc tcaaaattgg tgaaaaagta gcatattgga accatcttac cataagttct
    19381 ctaaattctg ggtctttagg atttcctcca tggaaacctc caatatcagt agtccaccat
    19441 ggtatacctg ccagtcccat atttaaccct gtatttactt gattattaaa agcttcaaaa
    19501 ctagaatcaa tgtcaccaga ccatactaaa gctccatact tttgagctcc tgcccaagct
    19561 cctcttacaa gagttacaac agactctatg tcattatgta aaccatcata aatcattttt
    19621 aaataatcaa taggataaat attaccacaa cttaaaacat ttcctttttt atatctatag
    19681 ttatcaaaat catacacaga atagccaggt tcagctacat ctaaccaata atagtcaatc
    19741 ccaagttgtt tataattttt atctattcta tcccaaacat attctcttgc attttcattt
    19801 gtcatatcta caaagactgt atttccttga atttgcattg tcattctaac accacgatta
    19861 acattgacta aatagccatt ttctaaataa tcattgtagt tttctgcatc actttgaact
    19921 gtaggccata cagatactat tggttctact gaatactctt ctttcaattt tttaactaat
    19981 tctttaggat ttttccaata atctaagtca aatttatatt caccttgctt aggccaatga
    20041 aaatagtcaa ttgcaattga tgaaagctta attcctcttt tagaatattc cttcacaaca
    20101 tctaaaactt cttctgaagt tctatatctt agtttacttt gccatagtcc taaaaggttt
    20161 tcaggcatca taggaacagt tccagtaact tgagaataat ttttatttaa ctcttttggg
    20221 ctttctccac aagtaatcca ataatctata aaatttgtag aaaacatctt ccattctgtc
    20281 atatttttag caaaagatac ttttccaatt cccggattat tccataaaag tccataacct
    20341 agactagaaa tataaaaagg aactgatatt tgagaatttc tttgggctaa ctccaaaaca
    20401 gtattcttta aatctaaaaa tttatgttga tattgtccca ttccaaaaat cttttcagaa
    20461 ggctcagatt caaatctagt agttacttca aattctccac tatagcttgg tcgatattct
    20521 cttgatttta actttagagt agagttaaag tctttggtaa tctcaattgt ccctacatct
    20581 tctccaccat catcatgttt tacagctctt aatcgaatat attctttcaa taatatttca
    20641 tttttatcat tataaaaagt aattctatct ctatggtcta agacagcttt tatctttcca
    20701 tttttaatga ttgcacttcc atcttcattt ttattaattg taatatcatc tttatttaac
    20761 ttaggttttt cgttaagagc atagttttca tcaacaaaat tttggtctac aaacgaccta
    20821 atacgtaaag agttttttcc ccatgcttct attcgcaata gttcattgtc aaatcttttt
    20881 tcaatatagt cttcagatat ttttatcacg gctacctcct aaatcaatgg tataatattc
    20941 tatctttgtg tagcttaagt taaataatgt cgtttagtat cgttatttat aaattaggtt
    21001 ttatatataa ttcatttgta cattaaacta acttaccaat aatatacttt atttgacttt
    21061 gtttgtcaat cgctttccgg attttagtaa aattttctaa tatattgctg gaaagtcgtt
    21121 cttaatatct tttatttata cttttaatat ctgtgatgat acctatatac atctagtttt
    21181 tttaaaacac atacaggaat aacatttgcc tattatgtag ataatatcgg acttgaaaat
    21241 atgtatagtg accttataaa tacagataat aatatattag atattgttca aaacaatgga
    21301 ttttataatt ataaataatt tattaaatta tttaagaaaa tatacaattg tataccaagt
    21361 caaaaacgac aacaaataaa taaaactaaa aaagatgttg tctcaaaata tagataaatt
    21421 tatttttcat tgctaaaatg attaaaatat atttgattta atatacttga tttaatataa
    21481 aaaaagagtg tctcataaac taaaaagttc acttttagac actcctttat atatattatc
    21541 tacttcttaa ttcatttatt acctctctac attttctttc aactagtgga tacatcttta
    21601 taagtactaa ttactccact taaaaatgat tcaggtactg tccatcagtc atatttttct
    21661 gccaaatata atggtgtttc ataatgagac aataaatatt tatatctcta cttatcttct
    21721 ctaagctcgt caaatccaac tataggtata tttaaattat attctttaag tagcttttct
    21781 agctcttcta taaaaatacc tttcccatcc accatttgta tatcatttat tttatccatc
    21841 aattcattgc aaccacttaa ctcaccaaac caacgtttag aactacatgt cttattataa
    21901 ccacaacttg gactgccatc tattgcaatg atacttttta tatcataatc attttttaaa
    21961 tattcatcaa actgccttac atgaggtatt aacatttgcc tacattgttc tctaaaaaac
    22021 aaattgtcaa actgttcttt tacatgtccc cacctattta ttccatacat aatcatttct
    22081 gggcatggta gttgaattat tccataccct ttatttatta aatctatcgc tatattttgt
    22141 gctcccttaa attctgacaa tccctctact ttagaattac aatttaatat acaatgacat
    22201 actactacaa tctctctatt tctcttcata ataatctcct tagttattct taattttaat
    22261 gatactacat attttgataa ttatattatt tatttgttct atatttccaa tattctatat
    22321 agaaatatac aattagtaat cttttataat ctatactatg ataaatgtat atattttttt
    22381 aggaggtatt ataattggat actaaaaaag tcacattttc ttcaatgtgc atagttatta
    22441 atattgtctt aggtatggtt atatctatga taaatatacc attattattt ttagatacaa
    22501 ttggtactgt acttggggct gtcgtattag gtcctttttg gagtgctctt attggtgggt
    22561 gtacaaactt agttttaggt gttatatcag gtcctacaaa tatacctttt gcccttgtaa
    22621 atattgttct tggtcttatt gttggatata tttctaagaa aaaaggtttt ggttataaag
    22681 aagctattat tacaggagta attttatcta ttgtttgtcc tttaattggt actcctattt
    22741 cagttttatt gtttggtggt ttaagtggaa gtggtgctga tttattggtt ggttttttag
    22801 ttcagtctgg tcagaagata tttactgctg cgttctttcc aaggattctt agtaatatag
    22861 tagacaaacc tttgtcttgt attatggtgg tatattttat aagtaaaatg cctaaaggtt
    22921 ttataaaaca atatagtaaa aatacttcta gttcatatta ataaatcatt tattatgtaa
    22981 gatttattac tctttaaaat gctatatact catttttata taacattttt attaataatt
    23041 taattagata gtatttgtat tattttaatt aaacgtaacc tcttgatata aatttgagct
    23101 ataaatctat atcaagaggt gctattttat ttttaatata attaatattc tattacgcaa
    23161 aaaatacata tatttcatta ttttatggaa aatattactt catttaactc tatatcaaac
    23221 tcattaagta aaaatctaat tatatttaat ttagcctctg tatttaaatt tgtttctaaa
    23281 tatatatttt ctgatatttt aatacctttt cttaatttat atgcatctct agacttaata
    23341 ttatctataa aatctctttt ataagtcttg tctacaatac tgttaaacct tacagaatcc
    23401 aaatcatata aagtaagagc aacttgttca taaaattctg tccaattttt agccttatat
    23461 gactctccta atatactaaa tgatactata ctttcatttg taaaattaac atcatcatcc
    23521 tctaacgtaa atatattttc actgttactt ggaggttcat atgtaacact tggatatttc
    23581 caaatttcta cagctctatc tagtaatatc ttagttctat tttttatttc ttcttcattc
    23641 catttatcta tagatgaaat atatttattt agataaagcc tactatcttt aaatccatct
    23701 tccatttctt tcttttcttc aaatgtttta ttacttagtt ttgaattata tcctgtcaaa
    23761 gttaaatttc caattgtatg taaatattta tcatgtatct ccttatagtt atctcctagt
    23821 gattccttcc atttatttgt taatgtctgt ggcattatat gctctatatt tagcatatta
    23881 ttattgatta aattttctaa atcaacaatt tctttattat tataattctc taacctctca
    23941 agtatatata acttattctt tgacttagta ttatatatat ctttagtttc aaaagcattt
    24001 gaaaattcac tgtcagtagg aaatctttga gatgatttct tatttataaa aatatatctt
    24061 aatatattag aatagttttc tttataatca ctaaattttt taatttccct tcctaagttc
    24121 ataaatactt tattcagtgc attaggaggt acatcacata ctattcttct taatataaaa
    24181 cattctacta aattaaggga ttctacaagt acttttttat ttattatacc ttcattataa
    24241 tcatcaaata attctaatat aaacggatat gatacaaat
//
LOCUS       DAEMGG010000053        23941 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEMGG010000053 DAEMGG010000000
VERSION     DAEMGG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.053"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1189)
                     /locus_tag="KPK24_001978"
     CDS             complement(<1..1189)
                     /locus_tag="KPK24_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2299650.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKIIELIDKDINIEMYYKNRNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEE"
     gene            complement(1176..1943)
                     /locus_tag="KPK24_001979"
     CDS             complement(1176..1943)
                     /locus_tag="KPK24_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299651.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            2231..2905
                     /locus_tag="KPK24_001980"
     CDS             2231..2905
                     /locus_tag="KPK24_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2299652.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            2929..3933
                     /locus_tag="KPK24_001981"
     CDS             2929..3933
                     /locus_tag="KPK24_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2299653.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            4045..5274
                     /locus_tag="KPK24_001982"
     CDS             4045..5274
                     /locus_tag="KPK24_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG2299654.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            5282..5956
                     /locus_tag="KPK24_001983"
     CDS             5282..5956
                     /locus_tag="KPK24_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299655.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            5959..7191
                     /locus_tag="KPK24_001984"
     CDS             5959..7191
                     /locus_tag="KPK24_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2299656.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(7477..8742)
                     /locus_tag="KPK24_001985"
     CDS             complement(7477..8742)
                     /locus_tag="KPK24_001985"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG2299657.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(8795..9943)
                     /locus_tag="KPK24_001986"
     CDS             complement(8795..9943)
                     /locus_tag="KPK24_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG2299658.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNKVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(10043..10588)
                     /locus_tag="KPK24_001987"
     CDS             complement(10043..10588)
                     /locus_tag="KPK24_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG2299659.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(10811..11755)
                     /locus_tag="KPK24_001988"
     CDS             complement(10811..11755)
                     /locus_tag="KPK24_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG2299660.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            12141..13022
                     /locus_tag="KPK24_001989"
     CDS             12141..13022
                     /locus_tag="KPK24_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG2299661.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(13083..14291)
                     /locus_tag="KPK24_001990"
     CDS             complement(13083..14291)
                     /locus_tag="KPK24_001990"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG2299662.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(14364..14616)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14768..15757)
                     /locus_tag="KPK24_001991"
     CDS             complement(14768..15757)
                     /locus_tag="KPK24_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG2299663.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            15909..16349
                     /locus_tag="KPK24_001992"
     CDS             15909..16349
                     /locus_tag="KPK24_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2299664.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            16676..16903
                     /locus_tag="KPK24_001993"
     CDS             16676..16903
                     /locus_tag="KPK24_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2299665.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            16929..19049
                     /gene="feoB"
                     /locus_tag="KPK24_001994"
     CDS             16929..19049
                     /gene="feoB"
                     /locus_tag="KPK24_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG2299666.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(19182..20000)
                     /locus_tag="KPK24_001995"
     CDS             complement(19182..20000)
                     /locus_tag="KPK24_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2299667.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            20207..20491
                     /locus_tag="KPK24_001996"
     CDS             20207..20491
                     /locus_tag="KPK24_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299668.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   20649..21206
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=20716..20744
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(21865..>23941)
                     /locus_tag="KPK24_001997"
     CDS             complement(21865..>23941)
                     /locus_tag="KPK24_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2299669.1"
                     /translation="EVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPEGKVCWQ
                     VLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQDGTVVHM
                     HQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDALIRGTDE
                     YIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSNTEIGKN
                     QMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKIDSLTQLL
                     NYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQSIQSIL
                     PDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITISAGCVI
                     YPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLKASINDD
                     FRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMINIVGAWV
                     LRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNLVLELTE
                     SHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRVFVKDIL
                     KSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGKPQTATE
                     IFDLLKLDN"
BASE COUNT         8484 a         3603 c         2935 g         8919 t
ORIGIN      
        1 attcttcata attgttattc tgatattgtc ttaatttttt aaaatcactt tctctaataa
       61 tatctacaga cgttctatct tctatataac tatttcgatt tttataatac atttcaatat
      121 ttatatcttt gtctataagt tctattattt tatcaaagat tatcttatta tcttttttat
      181 atttatataa taatttgtca atttcgttat ttacataaga atttcctaaa tttatggtat
      241 aactgtattt ataattttca cttatgttcc tatctatatc gtaataaagt gatatggtaa
      301 accctagcat agttacactt gtagctatta atattgctat tacagccaat acactactat
      361 tgctctttaa tttaaatcca agttcagaaa atgcaattaa attctgtccc ttataataaa
      421 aactttttct cttcttaaca gcatcaataa tcatatacat agttgatgaa aaaaataaaa
      481 aagttccagg tattattgta actaatataa aaactgaaag cataatattt tcagcaataa
      541 aataactaat tgacattaaa taagaaatag ctattaataa aacaccaagt acacatttta
      601 actttataaa ttttttaaac ttatactttt ctggatattt attaaataac tctataattc
      661 tcttatttct gataactata gtatttctta ttccaataac tatgaatatt attaaaaata
      721 ccgctagtgt ctgaactaag gcctttatac ttaagttcat atttactact atcatttcat
      781 tcattaattt tacaagcatc atagtcataa gctttgaaaa taacagtcct aaaaatatac
      841 ctattagtat tgcaactata cccatcaaaa aagtttcata taaaaatatt tttcctatct
      901 gatttttcct cattccaagt aaactataag ttcctacttc tcgcctacgt ctattgaaaa
      961 aaaatgagtt tgaataatat ataaataaaa atacaaatac cactattaca attgaacttg
     1021 tattaatact catcctagtt ttctctccaa gtgaactcaa cgcttcatta tactctatag
     1081 atttaaaact aaaaaataca aatacactaa atacaattga aataaagtac attaaataat
     1141 taaaaaaact attctttaca ttttgcattg caattctaaa taaagtcatt tttattaact
     1201 cctccaatta aggaaactgt atttactatt ttattataga actctctgtt actttctccc
     1261 tttacaattt ctgtgaatat tattccatcc tttataaaga gtattctatt gcaaaaactc
     1321 gcacttatag catcatgagt taccatcaat attgttttat tgctgtcttt gtttaatttt
     1381 aatagacaat tcaacaaatc cctagcagat ttagaatcta atgctcctgt aggctcatct
     1441 gcaagtacta atgatggttt tgttgctata gctctacata cagctcctct ttgcttttgt
     1501 ccaccagaaa cttcgccagg gtatttatct aatatagatt ctatatttaa ttcttttgaa
     1561 atctctttta ttctattttc tattactgat actctttcat tttttaaaga aagaggcaac
     1621 attatatttt cttttattgt aagtgtatcc aaaagattag aatcttgaaa tacaaagcct
     1681 agattatcac gtctaaaatc tgccaattca ttttgcttca ttttaatagt attctttcca
     1741 tttatataaa tatctcctga tgaaggtaaa tctattgtag aaataatatt taaaagtgtt
     1801 gatttaccag caccggaaga ccccataata ccaacaaatt ctcctttata aaccttgaaa
     1861 gttattcctc ttagagcttc atgttttgaa ccttttcttc catactcctt ttttatattt
     1921 tcaacagata atatttcttc catagttaat aatcaccttt catcacaatt agttatttat
     1981 caattctcac aaatatatta tatatattaa aatggctttt aaacatttaa ttatattaat
     2041 ttaaggttac attttcgtaa gtattaatta tatttcttca aatttattgc aattatgttt
     2101 aaaatataat ttatcaagta aaatatagtt atattaatat aattatttta ggtaataagg
     2161 tagaaatatt ttaatttatc aagtagaata tagttatgtt aatataacta ttttgaatta
     2221 ggaggtaaga atgtttaaaa taatggtagt tgaagatgac gtctcattga aaaatatcat
     2281 agcaaagtgt cttactaaat ggggacatga tgttcatcaa atagaaaatc ttgaaaatat
     2341 aatagaagag ttcaaaaact ataacccaga attagtttta ttggacataa accttccatt
     2401 ttatgatggt tttcattggt gtaatgaaat aagaaaaatt tcaaaagttc ctataatttt
     2461 tatttcttca agaaattcta atatggatgt aataatgggt gtaaatttag gtgcagatga
     2521 ttatatacaa aaaccttttt ctgtagatgt cttagtagct aaagttaatg cccttttaag
     2581 aagaacttat aattttgttg ataataactc aaatcagatt attcacaatg gtgttacttt
     2641 agatttatca actgcaacaa taaactatga agataatact attgagctaa caaaaaatga
     2701 aatcaaaatt ctccatgagc ttatgaaata taaggggcag atagtaagta ggaataaact
     2761 catgaaaaaa ttatgggata atgattggtt tgtagatgat aatacattaa ctgtcaatgt
     2821 aaatagaata cgctcaaaat taaatgaaat tggtttagaa gattttatag aaacaaaaag
     2881 aggattgggt tatataatca gttaaaacaa ttctaagtag gtgaatttat gagctttaaa
     2941 gattttctca aggataaact aggtttttta gtatacaatt ttactttctt aatttttaca
     3001 gtatcagtta ttatattttc tcctgtagat gtttttctga ctgatactat tttatatata
     3061 ttagttgtaa atgttgtgtt tttattttta tatctattta ttagctacat caagaagaac
     3121 aaatttttag ataagataaa aaatgataca ttcaaaatta atatgaatga tatagaatta
     3181 gctagatgta aaaatgaaga aaaaatatac ttgaacatta ttaaaaatca tcaatatcaa
     3241 tgtaataaca taataaataa taatatcgag aaatttgaag aaaacaaaga gattttaaat
     3301 atgtgggtac atgatgtaaa gatacctata gctataataa aactcattct tgaacaaaat
     3361 gaaaatttaa ttgatgaaaa aatttcagat gacatagata gtgaaatatt tagaattgaa
     3421 aactccgttg aaaaaatttt atatttatca agactcgaag attttcataa ggatttttta
     3481 attcaagagg taaatattga aaaaatcatt agaaatatta taaaaaaata ctcaaaatat
     3541 tttatttcaa aaaaaattat tttagactta aataatttga attttaaggt tttatctgat
     3601 gaaaaatggc tatacttcat attcgagcaa ttattgagta attccctaaa atactcctca
     3661 ttaggagata atatatctat ctattgcaaa agaaccaaaa attatttaca attaagagtg
     3721 gaagatactg gttgtggtat aaaaaaggaa gatttaaatc gaatattcaa taagggattt
     3781 acaggtaata atggaagaaa caattctaaa tctacaggtc ttgggcttta tttagtaaaa
     3841 gaactttgtg ataagcttgg tcataaaatt gatgtagatt cagagtacaa ccaatataca
     3901 aaatttacta ttacattcaa atgtgattta tagtatctta ttattgatag tttattttca
     3961 ttaacttata ttcaacttct tatagtaatt ctatatgtac atcccagtat ataattaagt
     4021 agtttttata aaaaggagga agacatgtct tttctaaaaa aatttaaaaa taaaagattt
     4081 agaaataaca gttttaaaag taacaacttt agaaatagca gttttggaag tagtggcttc
     4141 aagtctaaaa ataaaggttt taaaaacaat ccctttaaaa ataagagatt caaaatatat
     4201 gcaggaatag cagtaatcat acttttaatt ctaggagttc ttgcttatgt tgattcgaaa
     4261 aacactgaat tacaagctaa tgaaaatttt atcgaaacat atactattcc tgaaaatgaa
     4321 aaaatattta taaatgggat ggttgttcct aagcaaacta aggacttcaa tatatctgga
     4381 gattatgagt taagtgatgt taatgttaca aatggtcaaa aagtaaatca aggagacctt
     4441 ctttttactg ctaaaaatcc aactataata gcagaaatag atagtttaaa atcacaactt
     4501 agtcaataca aaaaacaaaa aatatcactt tctgatattg ctgaaaataa agatgctatt
     4561 gcttctataa atgcacagat aactgcttta aactctcaga tagcttctct ggataaaaaa
     4621 gcttatgaca gagttactgc tccttttgat ggcacagtat acttgaatga ccaaactgga
     4681 aatccagacc aaccagtttc atttatgact attcaaggtc tagagtttta tatgaaagga
     4741 caagcaagtg aacaagattt acctaaaatg aagattgacc aaatggtcaa tatactagtt
     4801 ttttcaactg accaaaagct tactggtaga ataagtttta tatctgataa accatctact
     4861 ccaaatactg aaatgggagc tcagcagaat acactatctt attatgatat aaacatagca
     4921 tttgataatc aagaaggatt agtaaatgga ttccatcttc aagcatcact tgaagtagct
     4981 aattcaagct ttaaaatacc agcatcttgt gtacttaaag acaacaaaca ctcatatgta
     5041 tttaaggatt tagatggtat attgaaaaag caaattgtag atgtagcaag tcaaaatgat
     5101 gattttgctg tagttagagg cggtcttgaa caaggcgata taataataaa acaccctact
     5161 aaagaaatga aagaaggaga cccagttcaa ggcgatggcg taactgctgg ttctaataat
     5221 ggagatacta ctccaaacaa aaaagtagaa gaagttacta tggatgtgaa ttaatttaca
     5281 tatgttaatt aaattagaaa atattcaaaa atattacaaa gtagggaaag atgaattaca
     5341 tgttttaaaa tccttaaatt tagaaattga atctggagaa tttgtcatga taatgggtaa
     5401 gtctggtagt ggtaaaacta ctttacttaa tatcttaggt tttctagatg tatttgatga
     5461 aggaagatat atatttgatg gaacagatgt aactaatttg agtgaaaatg aacgttctgt
     5521 ttttagaaat attaatattg gatttgtttt ccaacaattt aatttaatag aaactctaaa
     5581 tgtatatcaa aatgtagagt tgcctctaat ttacaataaa gcactaaaaa aatctaatag
     5641 agaagaaatt gttaaagata aattaagttc tgttgggctt ttggacaagc ttaagcagaa
     5701 accacttcaa ttatctggtg gtcaacaaca gcgtgtagcc attgcacgtt gcctagcaaa
     5761 tgaccctcag ataatctttg ctgacgaacc gactggggca ttagatagtg aaactagcag
     5821 agaaattatg gagcttttaa caaggctcaa caaacaaggt aagactataa taatggttac
     5881 acatgatcaa gatttaacta agtatgcaac taaggttata cgacttaaag atggtgtgtt
     5941 tacttcggag gtgtaatcat gagtttatta aaaaattctt tggccaattt aaaaggtcac
     6001 aagcttcgtg tatttgtagc tttactctgg attataatag gaataacttc agtaatactt
     6061 gttagttcta taggtaatgg atttcaaaaa gaaattaaga aatctgtaaa caatgtaaac
     6121 cctaataaaa ctactatttc gtttgaatca gccgataaca caggtcttac tgatgatatg
     6181 agtatttttt taaaaccctt taatgcaaaa gatttagaag aattatcatt tgttgaagga
     6241 gtagaaagaa tagctccttc aagagatggg tttaaccttg attcagttta ctcttcacaa
     6301 gcatcttttg ataaaaaaac tacgtatgtt gatgttggac cagtaaaaaa agattctaaa
     6361 ataaatttaa tttgtggtag agatttttct ttagatgatg aaaaaagaaa agtaatttta
     6421 cttacattac aaagtacaag tgaaattttt gaaaatccag aagatgcctt aggtcatggt
     6481 atcaacataa atggaactat atttgaaata ataggggtat tagatgattc tcagcaaaac
     6541 caagctggag gattctttgg aggttaccaa gatatgcaat ttactacttc cctagtacct
     6601 aaaaaggctt ttgacacttt aatgagtcaa aattcttatt caaatgaaat ttaccagtta
     6661 gacttagttt cttctaaagg ctataatgta aatgaagttg caaacaatgt tatagctaaa
     6721 ttatatgaga tgcaccctgg tatcaatggt tcttatacta caccagaccc aactgaacag
     6781 acagcttatc ttgaaagcat taattccaat gtaaacaaat atgtatctat aataactgta
     6841 gttgcaatgt ttgttggtgg tattggtgta atgaatataa tgtatgtatc tgttatggaa
     6901 agacaaagag aaattggtat aagacgtgct attggagcaa aacctaggtc tatattattt
     6961 caatttttag ttgaagctgt ttttataaca gtatgtggtg gaattttagg aactatagtt
     7021 ggatttgctg ctactaacta tgtttctaaa tatataggtt ttgaagctat acctagctta
     7081 aatagcttat tttatgcaat tgtagctact atattgactg gagttgtatt tggtctgatt
     7141 ccagccttta aagcttctaa actagaccct ataaaggcaa tttataagta gaaattgtat
     7201 aaagattttc gatttttaga ttagtttata taaaacatac ttaaatatat aacgcactat
     7261 tatatatgat agattaggaa cttaatcttt ccaaatataa tagtgcgttc tttcataaaa
     7321 aggattctct tccttactat atctaaatta ccaagatgtt atgttctaat tgttcaaact
     7381 aatacttcca attttgatta atcaatctca ttctcctaat atacaatctt ttactttcaa
     7441 tcattgtaca ctccgcctta aaaaaataat cttaaattag gcgttatata caatctcccc
     7501 attacatatt gtataattaa cctttccata taaagattta cctttaaatg gtgaattatc
     7561 agctttagaa acaaagcttt caactgtcca ttcttcttct ggattgaata taacaatgtc
     7621 tgctacagcc ccttcttcta taaatccaat atttaaattg tacaattttg caggatttat
     7681 agacattttt tctattaact gcatcatact aagatgattt ttatgcacca aatttgttat
     7741 tccaagagct aaagaagttt caagcccaat aattccactt ggagctttta taaactctct
     7801 atctttttct tctttactat gaggtgcatg gtctgttgct attatttcaa tagtaccatc
     7861 atttaatccc tctataatct tatctctgtc ccatttactt cttagaggtg gattcatttt
     7921 tgcatttgtt ccaaactcca atacatcttc ctcagtcaat gtaaagtgat gtggtgaagc
     7981 ttctgcaact acatttgcac ctaagtattt tgcaaatcta accatatcaa cagatacacc
     8041 tgaacttata tgttgtatat caactttagc accagtcttt agtgccaaca tacaatctct
     8101 ggctaccata acatcttctg ctacacttga tgcacctttt aatccaagtt ctgaagctac
     8161 ctttcctgca tttacacctg gatttccaac taaagaaggg tcttcttcat gaaaactaag
     8221 tggtacatcc aactcttttg ccttaatcat tgcctccata attagattgc tatccattaa
     8281 aggtaatcca tcatcagtaa accctggtac accagctttt ttaagagctt ccatatcaac
     8341 taactcttta ccttcaaaac ctttagttat agctgctgct tgtaaaacat ttatacatgc
     8401 attttttgac ttttctttaa cataattaaa agtatcttca ttatctacaa taggattagt
     8461 atttgccata catataacag ttgtaaatcc acctctagca gcagatttag ctccactttc
     8521 tatatcttct ttatatgtaa acccagggtc tctaaagtgt acatgtacat ccactaaacc
     8581 tggagcaact acattaccac tagcatctat tattttttca tattcatcac tttcatcaaa
     8641 cttgcctatt ttataaattt tattttcttt tataattaag tcaatattct catctctttg
     8701 acttttaggg tctattactc taccattttt aattaaaatc atattatttc tcctttatat
     8761 ttttaattta tatagactca tataatatta tacattattt tacatactta aatacaaaat
     8821 tacgtttgac atcaatagcc tttttaggac aaagttcatg gcaacagtaa catcttatac
     8881 atttacttaa gtctataata gcacccttag tccctatctc tatagtggat gcaggacaag
     8941 cttccttgca ttttccacat tttatgcaat cttgaaatct taccacaggc tttggtgtta
     9001 taagctttga aataggttca tttaaaaatt ttggtagctt tacggaaaac aatcttaaat
     9061 ctttacttac atttggaatc ttgtaatttt ttataattaa atcttttata tcttcaccta
     9121 aaatttcaat atctgaaaaa tcttcttgta tatatcctct cttaatagca cctctaattg
     9181 tagcaacttt atttggaagt aaacttataa tcttacatgc aacaacatct atagcatatg
     9241 gattttgtga tgctaataaa actcctattt ctcgagtagt accagctgaa ggtccatcac
     9301 cttccatacc tactatacca tccataattg tcaatgttgg ttttatataa tcacaaatat
     9361 ctaaaagtac attttcgcaa aacaactctt ctgtaggaaa tctataatgt atctctgctt
     9421 ttttcaatcc agctatgcat ccatataagt tttttgtacc accagtaaaa gttgccatta
     9481 tatgagtctt taatttacaa agatttatta tataatctga atctactatt ggtttaataa
     9541 tatccatata ttttagaaca tgtgcttttt cactatgtat cttaacatct gaaacatcat
     9601 aatttaactt aatgcccagt tcatttgcta cttcttcaat tccagatgcc ttataaatgc
     9661 tcttaagaga actttgatta tatggaccac cagggctatc tgctattgta atatcacaat
     9721 ttaactctaa aagtttttca catacaactt ttacaaccat aggatgtgta gttgtagctt
     9781 cttcaggctt ttttttcatt aataaatttg gttttataag tactttgctt ccttttttaa
     9841 caaacttatc tataccacca atatcctcta aatttttttc aatagataac tttacttctt
     9901 cataattgta agttttacaa ctcctaacag ataccgactt catattatct ccatccttct
     9961 atcacaacag tttatttata taatttaaaa aaggagaaac caaagtttct ccttttaatc
    10021 tatgttttaa ttgttttaat tattattttc caaaatgtct gtttaataat cctaagaatg
    10081 ctttaccatg tctagcttcg tctttacaca tttcatgtac tgtatcatgt atagcatcta
    10141 atcctaattc tttagctctc ttagctaatt ttaattttcc atcagttgca ccatactcag
    10201 catcaactct aactcttaag ttttcttttg tatctgcaac tacaacttct ccaagaagtt
    10261 ctgcaaattt agcagcatgt tcagcctctt caaaagctat tctcttatac gcttcagcta
    10321 cttctggata accttctcta tcagcttgtc tactcattgc taaatacatt cctacttctg
    10381 tacactcacc agtaaagtta gctcttaatc cttcgattat ttctgcatct acaccttgag
    10441 ctactcctat tctatgttca tcagcccaaa ccatttcgcc tttcatttct tcaaatttat
    10501 cagctccaac tttacatact ggacattgtg ctggtgcagc atctccttca tgtatatatc
    10561 cacatactgt acaaacaaat tttttcataa ttaatttcct cccaatttta tataattaaa
    10621 tttttatttt taagtttaat aacttgctac aagaatatat ataccccaga ctttatagct
    10681 taaacactaa aaataaaatt tatttcctaa ttaattttat cactttgaaa caccagtatc
    10741 aaccaagttt tgatgtcaaa tttctacata atacataatt tatttgatta attaggaaat
    10801 agttgtcata ttaaatactt tctaatgaag actcctctga atctttaagt ttcacttttt
    10861 tgctctttaa aaaagataat ttagtttctg cagttatgat tgcaacaaat atcaagatac
    10921 taccaataac caattttgct gtaataagct cctttaaaat tattatagaa aatattgtac
    10981 caaatacagc ctctgtggat aatattatag ctgttctagt tccatcaact cttttttgac
    11041 atatagtctg aaataaaaat ccaattgtag tactaaatac agctagatac atagttccca
    11101 tgtaaccaga taattcagct tccatcttta attgacctgc aaatgtttga actacaacag
    11161 acattaaaaa cgctactgta aattgaatag ctgtcaatgc cattgggttg ttatcttttg
    11221 caaactcact tgtaaagaat atatgaaatg caaatccgaa tgaacatata agtgttagaa
    11281 aatctccaaa attaatagag aaatcagctt ctaaagaaag tatcccaatt cctataagag
    11341 ctaccaaact acttattatt cctattttat caagttttct tctataaagt ataaatccta
    11401 tgaatggaac tattacaaca ttagctgctg ttataaacgc atttttagat ggtgtagtgt
    11461 acataagtcc aatagtttgt actgtgaatg ctagaaataa aaatattcct aaaatccctc
    11521 ctgcttttaa taatttttta cccatattgg cacgaatctg tttaaaaaac agtaaattca
    11581 taattatagc tgccaagaaa aatcttaaag ttatgatttg tagaggtgta agcccaccgt
    11641 ctaatgcaaa ttgggttgct acaaagccac ttccccatat aattgctata aatattagtc
    11701 ctatttctcc aaagtatttt ttcaataaag ctccctcctt tttatttttt tgcaaaatta
    11761 taaaccccct aaacagcaag agagtttttc tataagcatt actataaatt aatatagtaa
    11821 attttttcag tattttaaat ctaaatttac atacattttg agctcaaatt tatatccact
    11881 atttaacagt aatatattaa tcattttttg taaacacctt tttaataaat ttttatattt
    11941 tttatatttt tttacctata ctaaattttt ttatagatat ttttaatata tatgatactt
    12001 taatattatg caaataatgt catataagtt acactttagt tatcttagtt acaactttgt
    12061 cggtttactt tttctacttt cttcgatata attataatat atttttgata caattcgaca
    12121 aaagggggaa tgctgtaatt atggtaaaaa aaagaaaaaa gaaaactgtt acacttacta
    12181 tacttgttgt aatagttatt ggtatttttt ctatggcatg ggcggacatg tctagaaaaa
    12241 aagaaatgaa agaaactcaa gaaagactta aagctgaaag attagaaaaa gaaagaattg
    12301 aaaaagaaaa aaatgaacct attattggtg caaaaaaaga agctgtaaaa gaatatggat
    12361 atgacgcaaa aatagtttgg gacaaactta acaaatacga ctattcaaac aatggagaaa
    12421 agatagtctt tcttacattt gatgatggac catctactac taatactcca caagttctag
    12481 atatcttgaa aagacatggt gttagaggaa ctttctttat taagggtgat tctcttgaaa
    12541 gaaaaggagc taatgaaatt ttaaaaagaa catttgacga aggaaatgca atagcccatc
    12601 actcttatac tcatgactat aaaaaactat atcctaacag aagcctaaat ctcgatacat
    12661 tcgtaaatga acttaacaaa actgatgaag ctatgaaaaa agtcttagga aagaattttt
    12721 cctcaaatgt agttagatgc ccaggcgggt acatgtcatg gaaaaatatg gaacctctag
    12781 gaaactactt aaaagagaaa aacatggcat caattgattg gaacgcttta aatgctgatg
    12841 cagaaggtaa aaagaaaaat gcacaagaat tatttgaaca tgcaaagaaa tcttctgaag
    12901 gcaaagaaat ggttgttctt ttaatgcatg atacttatgg aaaacaagaa acagtaaatg
    12961 cattggacca aattataaca tattttaaag acaatggtta tcaatttaaa attttaatat
    13021 aataaaaaag caagtttatt gaatgtacat tttatactca atagacttgc ttttacttta
    13081 ctctatttta ctagctcgat ttttacaaat tttttctttc ctatttgaac aactaattca
    13141 ctctctttta caatagtgct taaatcctct actttttcac catttacctt tactccacct
    13201 tgagaagcta atcttctaac ttcactctta ctcttaacaa tctcattaga aactagtact
    13261 tcgattaagt caaatccatc ttccttaact tgaattgttt gtatatcttc aggtatttga
    13321 cctttttgga atacagattt aaatctttct tcagcttctt tagcagattc ttctccatgg
    13381 tataatgtta ctatttctct tgctaagttc atctttatat ctcttggatt tactgaaccc
    13441 tctttcaact gagattctat tttattaact tcatcaggat gaacatctgt cactaaattg
    13501 tagtatttaa ttataagttc atcaggaatt tccatagatt tttggtacat tattcccgct
    13561 tcttcatcta ttccaatata gttacctaaa ctcttactca ttttttcttt accatctaat
    13621 ccttctatta aaggcatcat tattactatt tgagactcca taccaaattc tctttgtaat
    13681 gatcttccca ttagtaaatt gaatctttgg tcagttccac caagttctat atcagcttct
    13741 aatgctatag agtcatatgc ttgcattaat gggtagaaga actcatgtac tgatattggc
    13801 atttgtcctt catatctctt tttaaaatct tctctttcta acattctcgc aacagtaata
    13861 gttgctgcta attttattac atcttcaaaa tttaacttag ctaaccactc gctattaaat
    13921 cttacaatag ttttttcttt atctaaaact ttgaaaattt gttcttcata agtttttgca
    13981 tttgcaagaa cttgctctgt tgttaaagct tttctagctt tagattttcc agttgggtct
    14041 ccaatttttc ctgtaaaatc tccaattatt attactattt gatgacctaa atcttgtaat
    14101 tgtttcatct ttctaagaac tactgtatgc cctaaatgta tatctggagc acttgggtct
    14161 agacccaatt ttactatcat aggctttcct tctttttctg atttaataag cttttccctt
    14221 aactctttct catctattaa atcatctaca cctttcataa ttattcgcat ttgctcgtct
    14281 atcgatttca tttttcattc ctcctaaaat atttatataa tctataattt ttatttttat
    14341 aataaaaaaa gctttcatcc tcttaaaagg acgaaagcat tgcttacgtg ttaccacctt
    14401 agttcatcaa tatctcacga tattaatctc ttagagtaca attacatata ctcgtgtact
    14461 ataacgtgta ctaatccgat atctcttaat ggttgttatc tttcagagat agtgctctga
    14521 gatgtattca aactaaatca atttattcca tttcaccaaa ctggaactct ctgttaaata
    14581 aaatctagcc ctacttcttc tcttcatagc atttatatat tcagattatt tcatcaatta
    14641 caagaaattt ttcttaactt actcataata tattattatt tttatatttt gtcaaccttt
    14701 tttattatta tatacatgtt tttttacatt atactacttt tttatatttt ttaccaatac
    14761 ccaaaattta tttttcaatc aaaagattta tttgtttatt gactatatta ctctgactca
    14821 atcttatatt tattttctta tttaaaaatg cgtttataat tattaaacta ataactgtta
    14881 catcaaatat aaaaaacagc aataaaatta ttatcaaaaa taaaattcta gcatataata
    14941 attttctaaa cttagctatt ataaagtttt gtagtgacat acatgagtct ctaatgataa
    15001 aatagacact tatataggtt attgatataa agattgtttt taagtaaaca gaatcaactt
    15061 taacattcaa cagttttaga acttcaaaaa tggctagata agaaacacat atgtaaaaag
    15121 ctttttttga gcaacataaa aattggttta aatttctaaa gttcttaaat attaaaaatt
    15181 tttttaaaga attagaatct aaaatatgca atttattccc taaaattaaa cttaataaat
    15241 atatgcaaaa caccatacca aaaggaaaca atgctatcat taaataataa ataaaatttg
    15301 tatacccatt aaaataaatc catttgctat atccttgaaa ttctttcata aaactataac
    15361 ctattaatgt tgaagcatat atatcaatga aaaccattac aataacccca aatactgaac
    15421 catatactaa atcatccata accattgtag gcttatttat tagatagcca cacattatac
    15481 caagtaatac actctgactt gcccaaatag cagttcctag atttccaagt accagagaaa
    15541 ttattaaaag cgaagtaatt ccagataaaa tggtatactt taaatcacat ttcaagcaga
    15601 ttatacaaaa aaatactggc actataaaat ctaagtaaat agcatatcca aatccagtac
    15661 tcacagctat aattgttact actatgaata aagatgacaa taaagctgct tcagttaatt
    15721 ttttagtagt attcaaaaca ctgcctccat aaatcataaa ttttctaact aaagttttgt
    15781 ttttaatata gtatatcact ttttaaaaat atttttaata aatataaatt ttaataaagg
    15841 tatttatgtt aaaaatggta taatgtatct taatattcag ttttttaaga tgctaggagg
    15901 taattactat gtttaaaatt aaacacttta atgatttgag tttagatgaa ttttatgaaa
    15961 tagcaaaatc aagatatgag gtatttgctt gtgaacagaa gatattttca ttaaacgatt
    16021 atgatgatat agataaaagc tcttatcaca tatttttaaa agaaaatgga ttgatttgtg
    16081 cttacgctag aataattcct aaagaataca gctcttataa tgatgtttcc attggtagag
    16141 ttttagttct ttctagtcat agaagaaaag gattagcaaa acaaatgatg gattgtgcta
    16201 tagattttat aaaagttaac ttacacgaaa ataatataac cttatcagca caaacttata
    16261 taaagaacct ttacttatct tgtggtttta aagaaatctc agaagtatac gacgaagcag
    16321 gtattgaaca tattaaaatg agattatagt gttgacaagc taatattgtt aatatataat
    16381 ttaagtataa attaaatatt gaattgatat ttaggtattt gaaaatacct tacatagagt
    16441 cagaaggctt ttgctttctg acttttttat tatcaaaatt caataattat atggattcaa
    16501 agtgttaatt tttattctat attctacttt ccccaaaagt aagaattata cccaactttg
    16561 tatgataggc atcttgccta tctttttttt gtaaaagtaa caaggtaata atataatgca
    16621 tagtttaaaa taagaagatt agcccaggct aaatttcaag catggaggtg ctattatgaa
    16681 aaaccttaac gatattcaag tgaaaaaaac tgtcaaagtt gaagatatat tatctagtgg
    16741 taatttaaga gaaagaatgt tagcgcttgg tcttactaga ggagctgtta tagatgtagt
    16801 tagaaaaggt ccaaaaaata atttaactgt ctataaaatc agaggtagca agatagctct
    16861 acgacaagaa gaaagtagct taatattagt ttctgacatt taaaaatttc tagcaggagg
    16921 atattaatat ggggttgaca cataactcaa ctaaaatgag ttctttaaaa gacatgtttg
    16981 atatagacaa taaggaagac cagtttgtaa ttgcattggc tggcaatcca aatacaggta
    17041 aaagtactgt atttaatcat cttacaggac ttagacagca tactggaaac tggccaggaa
    17101 aaactgtagc tacagctcgt ggtaatttta aatataaaaa taccgaatat gcattaatag
    17161 atttaccagg aacatattct ttatttgcct tatcacaaga agaaattgta gctcgtgatt
    17221 ttatatgttt tggtaatcca gatgcagtaa tagtagtatg tgatgctaca tgtcttgaaa
    17281 gaaatttaaa tttagtattt caagtgatgg aacttacaga taaagttatt ttgtgtatta
    17341 atttaataga tgaagctaga aaaaaaggca ttactattga taaaaaactt ttagaggatt
    17401 ctcttggtat tccagttatt ttaactgctg caagaaatgg ctctggtatg gatgaactat
    17461 tggatacttt aaatgatgta tcttttgaca aatataaatt aaataataaa cctgttagat
    17521 acaatgaaaa tatagagaat gtggtaaaat caattcaacc agaattggat aatattatac
    17581 caggtataaa ttctagatgg ttgggactta gacttattga tggcgatgaa agtatatttg
    17641 aatctatgag taattatata gacaaagatt ctatagatgc tattaatgaa gtaaagaaaa
    17701 agattccaga caatataaat aaacagaaaa taagagatga gtttactaaa atcaactatg
    17761 attatgctaa aaaactgagt gatgagtgtt gttctaatgt tgctaaaaaa tcaactgata
    17821 gagaagaaaa agtagataaa atattaacat ctaaaatatt tgggcttcca ataatgctct
    17881 tattattagg tactatattg tggattacta tagaaggagc taactatcca tccactttat
    17941 tatctaattt attactcgga tttgagccaa gtatttcggg tatattaaat agtattaatt
    18001 gtccatcatg gttaaatgat atgttggttt taggattata cagaacatta gcatgggtaa
    18061 tctctgtaat gctacctcct atggcaatat tttttccatt atttacccta cttgaggatt
    18121 ttggatattt gccaagagtt gcatttaact tagaccacct atttaaaaaa gcttgtgcac
    18181 atggaaaaca gtgtttgact atgtgtatgg gatttggatg taatgcagct ggagttattg
    18241 gttgtaggat aattgactct ccaagggaaa gacttatagc tatacttaca aacaactttg
    18301 ttccttgtaa tggtcgtttt cctactttga tagctatatc tacaatattt tttagctcag
    18361 ttataaccaa ctctttcgta tcaagcgtag caactgcact ttgcataact ttactgataa
    18421 ttctaggtgt tataataaca ttgttggttt cttatactct ttctaaaacg ctattaaaag
    18481 gagttccgtc tacatttaca ttagaacttc ctccttatag agttcctcaa ataggaagaa
    18541 ctttatatac ctctataata gatagaacaa tatttgtttt aggaagggct gtaatggtag
    18601 ccatacctgc tggtgtcata acttggatat ttgcaaatat atatatagga gatttaagta
    18661 tattatcaca tgttgcaaac tttttagacc ctttagctaa attaattggt cttgatggtt
    18721 ttatattact tgcttttatt cttggattcc cagcaaatga gatagttgta cctatacttt
    18781 taatggctta cctagcaact ggttctatga tagaattaga tagctttagt gctttaggtc
    18841 aagttcttag agaacatgga tggacttatt tgacagcttt aaatgttatg ctgttcagtt
    18901 tactgcattg gccttgtgca acaacacttt taacaataaa aaaagaaact ggtagtttaa
    18961 aatggactgc cttaggattc ttaatgccta ctatacttgc ttttgttgtt tgttttttaa
    19021 caactacagt ttacaattta tttatataat tagtattaac tgacagtaga catatattag
    19081 agaaaccatt taaaaattta tatactttat tataaagcat agagatatat tcaatgataa
    19141 gtaaaattac ttaacatttg aatatatctc tattttaatt cttatctgga tacttggatt
    19201 gaaatttcag tcatataatt atcttcatta ctttcacaaa aaaagtcaag tatactctcc
    19261 tcatacgaat ttccaacaat tttatattca ttttcatata tatatttttt caatttttcg
    19321 taagatttat atgaatcctc atattttcca atatgtttta tgcaagcata cattccttct
    19381 ggctttacat atagcttttt atcctttatt tttttatcta tcttcaaata aaaacttgat
    19441 atacttaaat agtccccatt ttttatattt tcattactta ctattactcc caactcatat
    19501 ccttgataca aatttttcga tttacaataa ttggtaaaat ctattaattc catcataaaa
    19561 tcactattac tcatattact caaatcgctt gatagaacta aatattcttc aaactttttg
    19621 gatatataaa tttcttcact attcatttta gaaattcctt caactgttat atttattcta
    19681 ttttgcatct tagaacttat ctttcttagt ttttttattt tttctcttat ctttttttct
    19741 tcttctttaa gcattttcac acaaagctgg ggagttctat tttgtatata ctcttttatt
    19801 tcctctaaac tcattcctat ttctcttaat gattgtaata tatcaagtgt atcatattga
    19861 taaactgagt aatatctata attattttta tctttatatt caggagaaaa aatacctatt
    19921 ttgtcataga aaattaatgt ctgcttagat ataccacaca atttagcaaa ttcacctgtt
    19981 gtaaaatact gtttttccaa gggttataca ctccttttct ttttgttgtc tatatagtta
    20041 ctatattctt tattatttac taaatcaagt agttattatt ttagaaaatc aatatggttt
    20101 tgattttaaa gcttaaatat cagttagatg tcagttataa atattttacc cattcccatc
    20161 ataaaaaaga tatcccctct taacttttgg atggaatatt atatttatac ttgttagagt
    20221 gaaaattgta ataggtgcta caataagata tttaattaaa cataaagtta attattgtaa
    20281 taaaaaatct ttagataaat tagagttaaa gtatggaaat atagatagag aaaaagctgt
    20341 taaactagaa atattttatc aatatttaat agaacttgaa tatatagaaa tgagtttatt
    20401 aataagaaga ttagatactg caataacatc attagcatta gtatctatta taacaatagc
    20461 atcatattat ttaatcagaa aaaatgtgta attatgtaag ctatttataa taaaacactg
    20521 ataaaattaa taataaaaag ataataggat ttctatacta cctttttgtt tttataatat
    20581 agtgtaattt tattatattt ttgtatgcta atataactag ataatcataa gataacacat
    20641 taaaagaaaa ttatattctg tatagttagt ataaaacgta agtaatataa atttatgtaa
    20701 agcaacaaaa gatggattta cattccatat agttaatata aaacgatttc aaatgattag
    20761 aataaaaata tcagaattgc tatttatatt ccatatagtt aatataaaac tataaagtgt
    20821 tacttttatt tattttattt tttacataat ttacattcca tatagttaat ataaaacgca
    20881 ttatatggct gtgtcatttt gcattctatt acatatttac attccatata gttaatataa
    20941 aacctgctta atgcagaatt aagagaggaa gtgttataag atttacattc catatagtta
    21001 atataaaact ggttgttcct gctccggtgt tggtggaact ggtacattta cattccatat
    21061 agttaatata aaacgaatat gtatggataa ttttttactt aacatattag catttacatt
    21121 ccatatagtt aatataaaac ctgctgtaat ttctgatgaa gcttctccac taccaccatt
    21181 tacattccat atagttaata taaaacccca aaataaactt agtattttca acacttacac
    21241 gtatacaact ctcttaaatt tgcagtgaac gagcaatagt gcaattgata acgcttatca
    21301 tacactctca acgccttata tttcaactgt tacgccaaat tttttcacaa atatcgctca
    21361 ctgcaaaatt cctataattt tattatatca taaatatatt acttttgaat attgatacca
    21421 atttatacca taataaaagc aaaaaaacgt aatctatcta cattagattt aaattctata
    21481 acttaaagtt ttttacataa tatttatgct tgtttaatca atctaatata tcattcttgt
    21541 atattgcttt taatagtctt ttttcttaaa taaatatcta tctgaacaag catagtttaa
    21601 ttaaaaactt ttgtattccc ttaaaactgt atctacctct tattatttta tttatgatat
    21661 attattttta atctaaaaat agatatctat ttttattatg gttatccctt tttaaaatac
    21721 tttaatcata tactattccc aatttaacat ccattgaaat gctattttct tatattgcaa
    21781 tttataattg atatatcttc aatataataa tttaatgtat ttttgatttt aaagcttttc
    21841 agtgatatta ttgataaatt agaattaatt atctagcttt aaaaggtcaa agatttctgt
    21901 tgctgtctgc ggttttccaa ataaatatcc ttgtatgtaa tctggcttta tttgtttaac
    21961 taaatcatac tcttcttgtg tttctacacc ttctaaacat acttttattc ctacatcatg
    22021 acaaattgca acaataaaat gtataaatgt agcatcaaac ttactcttta atatatcttt
    22081 aacaaatact ctatcaattt tcactatatc tattggagaa aattttaaaa cttctagaga
    22141 cgagtatcca gtaccaaaat catccatagc aatataaatt cctaaatctt gtaaagcctt
    22201 aaatttaaac tgtaaaatac tcatattttg tactgtatga ctttctgtca attctaaaac
    22261 aagattttga taaggaaaat tttcatcatc tataattttt actatatctt caataaatgt
    22321 gtcttctaat atttgaacag ctgaaacgtt tacactcact ttaaaaaaag gatagtaatc
    22381 aatccatttc ctaaaagtac gtaatgccat tcgaagcacc caagccccta caatatttat
    22441 catatcattt tcttctaata ttggaataaa ttccaaagga gaaattgctt tgcatttgtc
    22501 attagtccac cttaaaagaa cttctacacc tattatttta tgactttctg tatctacttg
    22561 tggctggaat cgaagactaa accccctaaa atcatcatta attgatgcct ttaaatcccg
    22621 catcatttct aatgaataca ttttattttt taatatttct tgggagaaaa aaacaagcct
    22681 gtttttacca tgctctttcg catattgtaa agagtaacta gcacacttat aaagttcttt
    22741 tactgtatct ccatgttttg gatatattac acaaccagca gaaattgtta tatttaatcc
    22801 atattttctc catagttgaa ggtgtataat catattctgt atttgattat atagagttag
    22861 aatttcattt tcttcaacat tatcaacaag aatgcccatt tcgtccccat caagtttata
    22921 aagttcagca ttatctggta aaatagattg tatagactgt gctagtgttt ttattatatt
    22981 atctccaaaa tccctatcat acatctcatt aacattttta aaatcgtcaa tatctaataa
    23041 tactatgcat aatttttcaa tcataggatt tgaaatttta tcttcaaata cactcataaa
    23101 ttcatgataa ttcagtaact gtgtcaatga atcaatctta ttttgtttac ctaattgcgt
    23161 cattattcct gcaaaaatac ttggttctcc aaattcatca cgcattagtt gacctctaca
    23221 tcttaaccat atatattctc cactacgatt tttagcccta aattccactg tatgataatc
    23281 catctggttt ttacctattt cagtgtttga cttataaaaa cgattccagt cttctggatg
    23341 tacaattttt ttccagtaaa caagcggatt ttcaacaatt tctcctggta agtcaaataa
    23401 ttcaacttgt gatggtgaat atcgaaatac gcctgttttc atattgcaaa tgtatatata
    23461 ttcatctgta cctctaatca aagcatcata aagcaatgta gaattataat caaaattgtc
    23521 tcttgaaaaa ttaaaaactc ctttttcttc tttattattt gatatttcat gaaattcatt
    23581 aatcttaatt ggtttattta agcttgttga gtttgcaata tctattgatt gatgcatatg
    23641 aacaactgtg ccatcttgcc atgtaattaa actatcataa ttttcaaaaa cacgatttaa
    23701 cttattacat ttttcatacc actttattaa tactcctttt ttatcattct ttaataattc
    23761 taaaacttta caaaaactac aagtagaatt tttttcagga tataatactt gccaacaaac
    23821 ttttccttct ggatctaaaa tattatattc ttctttcatt tttttgttca taaaaataat
    23881 ttcattagta tgaatatttg taacatatat attaatctgt agttgattta atataacttc
    23941 a
//
LOCUS       DAEMGG010000054        23462 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEMGG010000054 DAEMGG010000000
VERSION     DAEMGG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.054"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(32..1948)
                     /locus_tag="KPK24_001998"
     CDS             complement(32..1948)
                     /locus_tag="KPK24_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2299670.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            complement(1948..2694)
                     /locus_tag="KPK24_001999"
     CDS             complement(1948..2694)
                     /locus_tag="KPK24_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299671.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            complement(2798..3649)
                     /locus_tag="KPK24_002000"
     CDS             complement(2798..3649)
                     /locus_tag="KPK24_002000"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG2299672.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            complement(3897..4901)
                     /locus_tag="KPK24_002001"
     CDS             complement(3897..4901)
                     /locus_tag="KPK24_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2299673.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFAP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKGVNSLIKM
                     "
     gene            complement(4911..5603)
                     /locus_tag="KPK24_002002"
     CDS             complement(4911..5603)
                     /locus_tag="KPK24_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2299674.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            complement(5917..6876)
                     /locus_tag="KPK24_002003"
     CDS             complement(5917..6876)
                     /locus_tag="KPK24_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2299675.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            complement(6909..7787)
                     /locus_tag="KPK24_002004"
     CDS             complement(6909..7787)
                     /locus_tag="KPK24_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299676.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNKEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIRSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            complement(7780..8562)
                     /locus_tag="KPK24_002005"
     CDS             complement(7780..8562)
                     /locus_tag="KPK24_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2299677.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            complement(8563..9192)
                     /locus_tag="KPK24_002006"
     CDS             complement(8563..9192)
                     /locus_tag="KPK24_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299678.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIITECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            complement(9587..10213)
                     /locus_tag="KPK24_002007"
     CDS             complement(9587..10213)
                     /locus_tag="KPK24_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2299679.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            complement(10289..11260)
                     /gene="nrdF"
                     /locus_tag="KPK24_002008"
     CDS             complement(10289..11260)
                     /gene="nrdF"
                     /locus_tag="KPK24_002008"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG2299680.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            complement(11272..13371)
                     /gene="nrdE"
                     /locus_tag="KPK24_002009"
     CDS             complement(11272..13371)
                     /gene="nrdE"
                     /locus_tag="KPK24_002009"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG2299681.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            13849..14574
                     /locus_tag="KPK24_002010"
     CDS             13849..14574
                     /locus_tag="KPK24_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG2299682.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(14702..15898)
                     /locus_tag="KPK24_002011"
     CDS             complement(14702..15898)
                     /locus_tag="KPK24_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299683.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(15901..16926)
                     /locus_tag="KPK24_002012"
     CDS             complement(15901..16926)
                     /locus_tag="KPK24_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG2299684.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(16998..18128)
                     /locus_tag="KPK24_002013"
     CDS             complement(16998..18128)
                     /locus_tag="KPK24_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2299685.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(18356..18537)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(18590..19483)
                     /locus_tag="KPK24_002014"
     CDS             complement(18590..19483)
                     /locus_tag="KPK24_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG2299686.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            complement(19563..20465)
                     /locus_tag="KPK24_002015"
     CDS             complement(19563..20465)
                     /locus_tag="KPK24_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG2299687.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(20515..21675)
                     /locus_tag="KPK24_002016"
     CDS             complement(20515..21675)
                     /locus_tag="KPK24_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2299688.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(21831..22136)
                     /locus_tag="KPK24_002017"
     CDS             complement(21831..22136)
                     /locus_tag="KPK24_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2299689.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(22234..23208)
                     /locus_tag="KPK24_002018"
     CDS             complement(22234..23208)
                     /locus_tag="KPK24_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG2299690.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
BASE COUNT         7701 a         4249 c         2406 g         9106 t
ORIGIN      
        1 aacccttttt attatcattt aataatagta actaaaaatt gactaaattt aaccttatct
       61 cttttaaata attatttctt acaattaaaa aataaactat ttgaaccaag aacaagcttc
      121 ccattaccaa aacagctgat gatgctactt ctatatcaaa agaatttttc agtgcagtaa
      181 gtgcaaatat tgaatgtata acagccacta cataaggaaa caaaaatact attccaatct
      241 caattgttga agctttttta atctcattat atgtcatacc caatttattt aactgttcat
      301 actttttctt atcaacttgg caatccatat aaaatttatt atataaaaaa ctacttgtag
      361 taacaaagaa aattaaacct ataaatattg ctaaaaatat aaatgtagaa tacattaatt
      421 tcccagttcc tatcattaat tctttagaga aaaacaaata tggttgcatc cctgactcat
      481 tattaaattt attttcaaat tctctgcata tctctatagt tttacttgaa tcctctactt
      541 caaatgcagt aaatctatcc actataaatt ttgattctat actatcataa aaattatcct
      601 ttaccacata catatttttg taatacgctg gcataacaca ttcttccact tgttttgcca
      661 cctttaaacc tctattccct acaataatat tttctttgac gttcttattt cctggtaaac
      721 ttgatgataa taatgatata cattcattat cttcaaaact tataggctta acccctatag
      781 tttctgtaat ttctaagtac ttagattctt tcataattct gacattaaaa tcacttttct
      841 tgggaaatac agttttaaat tcaccattaa tccttgtata gtctacattc tcttttttta
      901 gattattttc tataaacatc attctttcct tgtctttttc tttgctatca ggctttttag
      961 ggtcattatg caatgtcatt gttgaataat atattgtgtt aggatatatt aaattaattt
     1021 gacgctctac atctttccaa aaggaataaa ctgtacctat agcagtaaat gccactgcag
     1081 aagttatagt tatcaagaaa aatattctag cattatcctt taccttataa atcaagttcg
     1141 aaatccaaag catatttgta ttttttctat aaaatctctt gtttaatttt agcttcttca
     1201 taaaaaatac actaaattga gaaaagaaca agtaagttgc tactatagta agggatgtaa
     1261 ctggaactaa gcgcagatat gttgtatatt ggtcggttga ggtaatagca ttataatacg
     1321 ctattataaa caaacaaata cttaatatag caagcattag tgatgtttta ggctctggtt
     1381 ttggagtttt tgtaccttta agtaacttca acacctgatt ttctttaact ataaaagaag
     1441 taaatattga tgttaataca gctaaaatca taaaatagat taaagtagac aatatcgcat
     1501 taaatggtat atagaatttt aatggtgata attctaaaaa agtagacata accactaaaa
     1561 atatctttgc aaagattaag cctattatta ttccaattac agaagatact atatttatta
     1621 tcatattttc tataaagatt aatttcatta cttggcgctt tgatatacct gtaatataaa
     1681 gtattccaaa ttctttatat ctacttttaa gaaacacact aactgaataa aacacaaaga
     1741 agaataagaa tagatatgca attatcttac tcatcttgag accttctttt aaataatcag
     1801 gaaattgaga tacatctaaa tctggatgac ttgtaaacat aataaaagaa aatagcagtg
     1861 aagatgatat aacaatactt gctaagtatc ctaaatacgc tttaatattt cttgaaatat
     1921 tgttaatagc aaactgtttt atattcatta gttatcgcct cccaataatg taagtacatc
     1981 cattatctct tgataaaact gctctctact actaccctta tatatttcat tatatataga
     2041 cccatctttt atgaataaga ttctactaca gtagctcgct gctaatgggt catgagttac
     2101 catcatagta gtaactttat tttctttatt tatcttttga aatagctcca ttacaacctt
     2161 tgatgacttt gaatctaaat tacctgttgg ttcatctgct aaaagtattg atggattgtt
     2221 aatcaatgct ctagcaatag cagtcctctg agcttgacca cctgatactt caaaagttct
     2281 ttttcctaat aaatctttta tacctaatat tgtagatact tcattcaatc tctcatcttg
     2341 ctcttttaca ctgaccttat ctaatgtcaa tggcaatact atattttctc ctatagttaa
     2401 tgtatccaat aaattaaaat cctgaaatac aaaacctaac tctcttcttc taaataatgc
     2461 aagttcctca cctcttaaca ataaaggatt cttgcccttt aattccatag ttcctgtagt
     2521 tggcttgtca atagtagaaa tcatatttaa aagagtagtt tttccactac cagatggacc
     2581 cataattcct acaaattctc catcttctat actaaaattt atgtcattta atgcgttgaa
     2641 aattattttt ttaccatata ctttacttaa attatttaca gttaaaattt tcattattta
     2701 actatccttc ctaatttaat ttttgaaata acatatatat aattgttaat tataatttat
     2761 cttagtttaa tttttataga taataaatct attttttcta tacactaatt atgttagtaa
     2821 aagctaatac tgcaagtact acacccatga ttattctgta tacagcaaac actctcattg
     2881 gttttttctt taaaaaattc acgaattttt ccataactat tattgataca ataaaagcta
     2941 ctataaaccc aactaccaaa gcaatccaca atgttggtgt cattattgaa taatcaaatt
     3001 caaataaatc ttttcctgaa gaagccacca tagctggtac tgctaaaaag aaagaaaact
     3061 cagctgctgt aggagaatta agtcctgcta tccatccacc cataatagta gatgcactcc
     3121 ttgacatacc tggccatgca gacaaaactt gtaatgtccc aacctttaaa gcttgtattg
     3181 gagtaatttt atctatatta tctgtggaat gattcttttt tctaaacaaa gtttctacta
     3241 ctaataataa aatcccaccc actataaatc ctataattac agattgtaaa ttaaacaatg
     3301 attttatatt atcgtaaaat agaactccta taatcgcaaa tggtatacaa cctataatta
     3361 cattcattcc aaatctaaaa ccaatctttc cttcttttcc accagtaaat ataaatttaa
     3421 aaaattctat aacgctatct tttatctttt tccaataaag tactactact gctaatatag
     3481 ccccaagttg tattacaacc tcaaacattt cagcaaattg ccccttaaaa tctattaaat
     3541 taccaaccaa aatcatatga cctgtagatg aaactggaat aaattctgta agcccctcta
     3601 ctatagctat tatgactgat ttcaatataa aaataacatc taaactcatc aataaacatc
     3661 cttccttttt tacaaaattt ctttttttat gttatttctc ttatgttatt tcttatatta
     3721 attgtaattt agattagaaa atttaactat ctgcaaacat tacacaaacc ttacatctat
     3781 gtaaggtttg caaataaact ctcttaaatt ttgattattt aactatgaat atttcattta
     3841 ataaattgta taataaaaaa gtacaattaa ctaaaaaccc tattgtaaca gataatttac
     3901 atctttatca aactatttac acctttatta aagtagattg aaacttctgt accttttcct
     3961 acttctgatt ttatacttac tttatgacct aaatcatcac ataccttttt tactagataa
     4021 agacccatac ctgtagattc tccaaactta cgaccatttt ctcctgtaaa aaatggggca
     4081 aatacccttt ttaaatcttt agaaacaatt ccaattcctt catctattat acttaaaatt
     4141 acatatttat tattttcttc agattttata gttaggtagt tactataatc tttagaatat
     4201 ttcactccat ttgtaattaa ttgacctatt ataaatttca tccattttac atctgtataa
     4261 acataagtaa acttgtctac ttgaatctta ggaatcattc cactttttat aaataacctt
     4321 ttttcttcat ttacaatact agatacaatt tcatttaatt tggctttttc aactataaag
     4381 tctttctgaa aattatctgc ccttgcaaaa tgcatagcca tatctaaccc tttattcaat
     4441 ctatctattt cttttcttat attttcagtt acttcttctc cctcataatc ttggagctga
     4501 aggcttataa ctgatattgg tgttttcatc tgatgaatcc attggttgat gaaagttaaa
     4561 tgttcactat gtatcttttt atgctcttgt atcattgatt gatacaattt atgttgttgt
     4621 tttaaaataa ctgatatatt tctccccaag aatgagcttc ctaaatacaa aaatgactcc
     4681 tccaaagaat gtactccttt ttcaaacaga ttatatactt ctctattttt ataatactta
     4741 tatattaaaa aacataacaa tataaaagaa ctaaataaaa gaatatataa actctctcca
     4801 aaactaataa acttcataag attacagtaa ataattgtca taactatgcc tatataatat
     4861 ataaaaatat aacctttata atcttttaaa aataacttca tactttctcc ttaccaattc
     4921 ttatttaatt tatatcctaa ccctcggaca gtctgaacag cattatctat acctaattcc
     4981 tcaaatcttt tccttatcct gctaacattt acatttaaag tattttcctc tacaaactca
     5041 atatcatccc atatcttttc taaaagataa tctctactta ctacttttgg gtatctcttg
     5101 ataagacatt caatcagtat accttctttt ttagttataa ttagattctc accatttaat
     5161 tctagttcta acctttctgg ataaaatttt aatccatcta gctccactat tctttcgtct
     5221 attttaggtg aatattcacc atataccctt cttatatgac tctttatttt tgccattact
     5281 acatcatagt aaaaaggttt tgttatataa tcatctgctc cactttctaa agctctaatt
     5341 tggtctacat taccatctct agctgatata aaaattatag gtaaattcga ctgttgccta
     5401 atacatctac accagtagaa accatcaaat ttaggtaaat ttacatctaa tagaactaag
     5461 tgtggcttaa aactttcaaa tgtaatcatt atatcatcaa agttctcttc aacaatcact
     5521 tcaaatccat atttctcaat ataatcttta agtaatgtac ttattgatat gtcatcttcc
     5581 acaatatata ctttataact catataattc ctcctaatat acctatttac tcatcatata
     5641 acagtccaat tttaatatat tataatcttt ttacaatata taaatttaat catattacta
     5701 acttcacaat tactgacttc ataagtataa ccatattatt agacatctta ataaaactct
     5761 ataaaaaagt atctttttat gattaattaa tcattattat caactaatca ttctaaaata
     5821 aattgaaatt actaattcaa aatagaaaaa gttaccttaa taaggtaact taaactttta
     5881 ttatatttct tacttagtat tattttatca tagtttctac ttagcattat agattaattc
     5941 ttttatgttc acttttttct cttgtaactt attttttatt aagaagtctt gtgtatcttc
     6001 aagcgaagat atgtctttat ctgttatatc taaactaaaa tcataccaag catataattc
     6061 tttagtttct tcaagactta aatcaacttc tttagctact ttttccatag cttcatcaaa
     6121 attattattt acatattcta aagtttcctt ttcaaccttc ataaatcttt caactatttc
     6181 tggatgtttc tctgcaaagt ctgtgctaac agcagttact attataccat ctactaaacc
     6241 ttcaccattt gctacaagtt tactacctga cttcatagct ttaagagctg ctggcccagc
     6301 aatcaatgca gcatctacgc tgccatcaga aagagctgct gatgcctctg gaatacccat
     6361 attaacaaat tccacatcat ccatacttaa tccttcttta tcaagtgctg aaatcaatac
     6421 ctgatgtaat atagtccctt ttgggcctgc caccttctta cctactaaat ctgctgctga
     6481 ttttattgaa ttattattag tcaaaatcat aaatcctttt ggtgacctgc tataagtgtt
     6541 taatatcttt aactctactc cattagatgc agctattaaa gctgatgtac cacctagtgc
     6601 atgtaagaag tctatttctc cagcagctaa agcttgtgtt tgctctggac ctgtagtaat
     6661 ttcgtggaag ttaactttta tattatcctt tttaaattcc tttccaaata aatcatcctg
     6721 tttctgaatt atagaaggaa catttaatgg tgattttaca taagttaaat ttatttctga
     6781 aagtttttct ccattatttt ttttagcaca tccagctaaa gatgctgttg caagtgttaa
     6841 tatcgttata cctatcaata gcttttttaa tttcatttac tttactcctt aaaatattat
     6901 taatttcttt aaatgccttt atttgacttc attgtcatta aaatatcttt ttttaattta
     6961 atatagtaat ctgaagttaa atctctatta taaccatctt ctatattaaa ttcttttact
     7021 tttctttcgt ctgtgaaaac aactattttc ttgcctatct ttaacgcttc atcaatatca
     7081 tgagttacaa atatcacgcc tttattagtt gacttatgta ttcttataac ttcattttgc
     7141 atatcaatcc ttgtaaaata atctaatgcc gaaaaaggct cgtccataag caacatatct
     7201 ggattgaaag aaagagctct agctatgctt actctctgcg ccatacctcc tgacaattca
     7261 tttggataag agtttttaaa tttctgcaag ttcatcatct taagatattt ttctacatca
     7321 atattacttt tcctattata tttatttgat ttgttcaaat tatcatttgt ttctaagctt
     7381 ctaaaattat catttataat tttactccta attcttttat tgtgtttaaa ttttctaagc
     7441 accttgttta aaatagtatt ttttctttga ttatgaaaag ctatattttc acttacgttt
     7501 aaccaaggca ttaatctact ctcctgaaaa acaaaaccaa cattaggtct atactcttta
     7561 ttatcattaa taaacctaat ttcaccacta tttatacctt caagtgatgc aattgctcta
     7621 agcaaggtag tctttccaca accactttca cctaaaatta ctgttatatg gtttctatct
     7681 atgtttaaag agatatcact aagaactaca tgttctttat tgtcaacttt ataactttta
     7741 tataaatttc taattatata tccagagtca catactaaat tattcatatg cttccaccat
     7801 ctttccctta cttaccttct tcacaataat cgaaaatatg taatctgtaa ttattcctaa
     7861 aagtccaatt acgattatcc ctacaataac aacatctgtt ctagacattt cttttccatc
     7921 agatatcaag tatccaagac cagatgaagc tgctacaagt tctgccccaa taattgctct
     7981 aaagctatat ccaagagcta actttaaccc aacagcaata tcaagaatgg catttggtat
     8041 tattatttta aatattatct gaagctttga aaactcaaat actctaccca cttcaatcag
     8101 ttttgagtca caatttttaa tccctttcaa tgtgcttgta aagattggga aaaaagatgc
     8161 taaaactata attataattt ttgattcctc tcctatacca aaccataata taagcattgg
     8221 tattaaagca aggggaggtg tattccttaa aaaatttatt agagatttaa agtattcgta
     8281 tactccacta taaattccaa agaatatccc taaaggcaca cctatagcag tacttattgc
     8341 aaaacctatc aaaactcttt tcatacttgc atatacatta ataaatattg aaccatcact
     8401 tatcatattt aaaaatgtac tatatacctt ttcaggtgat ggtaatatat aatcactcca
     8461 aataccaaga taatttgtaa ttttccataa taaaagaatt ataaaaaata tcagaacact
     8521 ctttactagc cttaaaaact tattattaaa tttattgacc atttacaaac catctccatt
     8581 attacaacca tctttacaat aaaacatttc tactatgtca tacttatctt caaaaccatt
     8641 tcttattttt tgtatttcac ttaatatttc gtcttcactt gatacttcaa gaactttttc
     8701 aaaagtatct ttgaaatatc ctaatggaat tggtgattca tctatttttc caagtgattt
     8761 tacaccttct tgttcagcaa actctttcca tgtataaaac atagcattac ccttatcctt
     8821 aaacttttca cttgcaaagt ctcttaattg tgtacatgga caggttacta ccaacatatc
     8881 atctttactt tttatatatt tatcgtataa aaccctacaa gtccttacta atattggttc
     8941 tatcataggt acttcaaaat tatttatcaa attatttctt tttaaaagac ttatactttc
     9001 tggacatcta caatccaaca ttgtattttc agtctttttt gtatactctt tgtactgctt
     9061 ttttacatat tctaactgtg gttcacattc tgttattatg tacccctttt ttatcaaatt
     9121 tgctttaata aggtgaaact tttctccata cattctttct gccactggat ttattagaag
     9181 atatttattc ataacgcctc ctattattta atattgaata ttattatcaa ttaatagttt
     9241 aaatgagcac ttaaattttg tcaatatatt aattttttct taataaaaca atttttatct
     9301 acatttttta atattttaaa tgaaatatta aatagtatat gtaatttatt ttaattaata
     9361 atgcatacat cttactaata atctatttat ataataatct ataaatatta tataataata
     9421 cttatataat tagtattatt atttaatagc aacagttatt cacactaata aaagtagaaa
     9481 gtttttaaaa tattatgaaa catcaaattt aattttaaaa ttgcatcaaa aaatgaagtc
     9541 tcaacagact tcacttttta taactcttat aaattattca aaatatttat acttcccttt
     9601 ctacttcaaa acgtaaaacc gtcttcaatg attcaggcga tacttttcta gcatcactta
     9661 tataaatttc acgatgtgtg taagattttc ttataagttt attttttttg caaaattcgt
     9721 tcataatgtc aaagctttct ggttcattat catatgaacc tatatgaagc atttgaacac
     9781 atagcccttc attaatagac ccaaatctaa ctttttcaag aagattatga ggttttttct
     9841 tctttactat atcagtagct ttatccaaca ggtcttgtgt aacaaaatct ggctgacgta
     9901 tcattatagt gtaaattaaa tcttctttaa taagtacatc taagccttta cttttttctc
     9961 ccttatccca tataccttct aaaggaaaaa ccgtatagtc aaaataccct tctggtacaa
    10021 ttcctttttt aggcatggtt tttattgcat atgccaaaga ataaagtact cctattgctt
    10081 cagtaaattc tttagaattt gggtctccac tcccttttaa catgataaat ttctgttctg
    10141 gtatttcaac tattacagga ttctttttag gtaaatataa ctctttatct ctttttctcc
    10201 attcatattt cataaaaata cctccatata tattttatac attgatatta tcacgctaaa
    10261 agtgacaaca gtgtgtcata attataaatt atattataaa atcttcatcc tctaattcct
    10321 catacactcc tttttggtat ccattaccct tagttgaaaa aaaatcatgt gttttggttt
    10381 cagtgctaag accatttaat acaattggat ttatagcatc tacagtatat agcttctcaa
    10441 accctagatt ttctaaagct ctatttgcat tgtattttaa aaagttcaca acctcttttt
    10501 caagcccact ttctctgtat ataacatttg tatactctat ctcattctcc ataaggctat
    10561 aaagtataga atacatcttc tcttctaaca tctccttatc cattttatca aatgaatcgt
    10621 atatttcttg tgctagcaat cctatatact taccatgtaa agactcatct ctaagaataa
    10681 gcgatattat ttctccacta gctaccatct ttccttgacc agatagatac aatggataaa
    10741 agaatcctga ataaaacaag aaactctcta aaaatacact tgtaaccatt gataggtata
    10801 atccctcttg atttgtagta ttttcgtatt gagctaaaac taaatcagcc tttctttgta
    10861 atgttggttc tgtttctatc cattcaaata attcatctat ttctaaatta gaaagtagag
    10921 tcataaatat agaagaataa ctttttgcat gtatttcttc catagtcccc ataaaagaca
    10981 agacagcttt tctttgtagg ttttctgtta aactcatcat agatggtatt ccattattcc
    11041 cctgttttgt atccagtaat gttaaaccac ccaatacttt tttatataat tccttttctt
    11101 tattacttag ttcattccat acctttatgt ctttagaaac agatatttct tcaggaagcc
    11161 aaaattgttt tacattttgt tcccaaaaag cttgtgtaaa tccatcttct tctctattcc
    11221 agttaactgc attatgtatt ttatgtaaat taaatgtcat aatttctctc cctatactga
    11281 acacactaga cactcatgag tatctcttgt catctttgtt cttgtgtaat ataaactttt
    11341 caaacctttt ttataggcat atatgtaatg tctagctata tctctagttg tcttttcatc
    11401 tgtaacaaac agtgttgttg atataccttg gtcaacatgg ttttgaactg ttgccactaa
    11461 atctatgacc tttctcatat ccatcctgta tgctgattga taatacaaca tattatcatt
    11521 tgttaaaaat ggcattggat atattgtagt agagtctcca tatgttctta cttctactgg
    11581 ctcagtaatt ggcattatac ttgatgtagc attttgaaca taactaatgc tttgagttgg
    11641 tgctactgcc attaaatatg cattatatat ccctttttct tttacctcat ccaataattt
    11701 tgtccagtct tcttttgttg gtatataaat cccttcaaat aatgctctta ccttttctga
    11761 cttaggtaga taagattgtt catagtattt tgataaaacc ttgctatttc ttcccttaac
    11821 atattctgat ttatcaaatc cttcaaaagt ttgatttctt tctatagcta ttttcataga
    11881 agctttaatt gaatagtatc taaccattgc aaaaaataca ttagaaaaat ctatagcatc
    11941 atctgaagta tataaaatat tttctcttac taagtatcca tgtaaattca tagctcctaa
    12001 tcctatagaa tgagacttgc tgttactatt gcttaccgtt ggcactggtt ttatatctgt
    12061 gctatctgct acaaatgaca gtgctcttat tgctgtttca actgtacttt ctatagtttt
    12121 attatccatt acattagcta tgtttaaaga acctaggtta caacttatat cttgtcccca
    12181 ttcatttttt cctccatacc cctctatttc tgaaggtgtt tgatattgaa atatctcaca
    12241 acataagtta gacattttaa taattccaac atcttttaat gtatgttctc tattagctgt
    12301 atctatgtac actacatatg gatatccact ttcttgttgc atctgtgcta tttttgtcaa
    12361 catctgccta ggatttattt ctttttttct tatatcacta tcattaacca atttatcata
    12421 ccactcatcc atttctatct catcaagaga aactccatat ttcttataca cagtatgagg
    12481 ataaaatgca tatgctactt catttttttc tgccaactcc ataaatttat ctggtattat
    12541 agctcctaat gataatgtag caagccttat tttttcatct gcatttatct ttttagtatc
    12601 catcaacaat tcaaaatcag gatggaatac agtaaggtac accgcaccag aaccttgtct
    12661 agctcccatt tgattaaagt aactaaatga ttgttctaac attttagcta ctccaacaac
    12721 acctccagct gcaccttcta tatctttgat agattcgcca gatgctctaa gtttagataa
    12781 atttagtgca actcctccac caatctttga taagtgattt gatgaagata tagcatatga
    12841 tataccttct gtactatctt ctacagatag taaaaaacat gataccattt ctccagccct
    12901 ttttcttcca gcatttaaaa aagtaggtgt agctggttga aattcttgtt ttactatttt
    12961 atctgccaag tttaaagcta aatctttatc accatttcct agtgtcaaag ctacaattaa
    13021 cactttatca ttatatgttt ctaatatttc ctttccatca ttgctcttta atgcataatt
    13081 ttgataaaac ttatttgcac tcatatatga ttgaaattca aacttatgtt tatcaatcat
    13141 atcatatata tttttaataa catctatgct atactgattt attacttctt ttgaataata
    13201 accttcatct attaaatact tcaatctttc ttctaggtta ttaaattttc tcaactttgg
    13261 ctgtataaat tcttctatgt aactacttaa ggcttcctta tccttttcca attggtactt
    13321 gccttgttca tttttaatta tcacttgatt gttaagctct atccaattca ttatttaccg
    13381 tccttttaac ataatcttgt gccataattt cacttaattt gcttattatt tcttttggta
    13441 cttatcagtt gaaatcccaa aattaatcta taattataga aatgcaataa gtatttttca
    13501 aaaacaaaag cactacatat agtcagtgct ttatctatta tatactacat atagtatata
    13561 cacaaatttt tttatatcta aacttttatt actttataat tgacattatt tttatttaaa
    13621 gaaattttta tcaactcaaa tataaaacaa tacaagctta ttatgtcctg tttaaaaatt
    13681 taaattatct ctaaatagct taaaaatata ttctgatata ttgaaaaaag tactttcaag
    13741 atgatatttt atatattatt tagaagtttt ttatttattg ttatataatt aaatatatga
    13801 ctattttgct actacatata taaaaataaa tttatgaagg tgattttaat gaaaactatt
    13861 tctaaacaag aattagaaaa aacacttgag aaacattatt tatggcttaa aaacaataat
    13921 gctggagaaa aggcagattt aaaagaatta agattagaaa atatggactt aagaggatac
    13981 tgcttaagta atattgactt ttcgtggtct gaccttatca atttaaattt agaaaaagca
    14041 gatttagaaa actctatttt taataattct tatctttcgg attgttcatt aaaaaattct
    14101 atattaaaaa atgcagactt aagtggtgca agttttagat tttgtgactt atctaactca
    14161 gatattagag gagctaattt agaaaattca aatttagaac atgctacttt aaatggagta
    14221 atatcagatg aaagtacccg tttttttaaa ttgtattgtc ctgaaacagg agcatttata
    14281 ggctataaaa aatgttttaa ttttagaatg gtacaattat taatacccag cgacgcaaaa
    14341 agagtgtctg ctacatcaaa cgcttgtaga tgtgataagg caaaagtact gtcaattact
    14401 agtatagatg gtaaagaatc ttttaaaagt gctcgtgcat atgctgatga aaattttata
    14461 tatagagtag gagaaacgat tgtagctgaa gattttaatg aaaatagatg gaaagaatcg
    14521 actacaggga ttcacttttt tttaactaga gaggaagcta ttggttatct ataaaatgct
    14581 aaagttttat aaatatataa atacataaac aaaataacaa aagagtatag attgtaattt
    14641 caaaaaaatt ataatctata ctcttttttc tatagcacat aagtacattt tatttcataa
    14701 attataataa attatttata tctgcactat tgattatttg agaaacttta tctatatgtt
    14761 ctatcttact atccaaactg tcataatact tcttactctc atctctattt ctaaaagaat
    14821 aaactttttt atccaatttt aaagcctctt ttataatatc tatattcctc ttatttgtct
    14881 ctcctacact aaatcctgta tctattataa tcttggaatc atttaaattt ctaatagcca
    14941 aatcgaaact ctcatcactt ataggctcaa atggattttc tgtaaacatc tttatcccca
    15001 tagttctacc aatttcataa tcaatatcat tttcatgtat tattccagaa tacagaccta
    15061 tacctttttt agtaaaagct ctataaattg gtgttgcttt acccccacca ccaattataa
    15121 aaacttcatt tttagattta ttactaattt ctactgaacc taaaagacta ttaaagtttt
    15181 tatcatccaa ttcataaagc gaatttataa tatcctcatc tactacatct tcaggctgac
    15241 cataagctat gactttatta ttttttataa gagcaacttt atcacaactt tttaatgcta
    15301 tgtctatttc atgcaatgaa agtattactg atattgactt ttctttactt aattttttca
    15361 gtatgtttat gagttccaat ctatgtttta tatccaagtg agttgtaggc tcatctaata
    15421 taattatttc tggttcttga acaagagccc ttgcaaccaa caccttttgt ttttctccat
    15481 cacttagttc atcaaaatat cttttcttta aatgtaaggc atctacagac tccaatgctt
    15541 catctaccat tattaaatct ttcttagata gactcccaaa aaatcctgta tatggatacc
    15601 ttcctatatt gactatatct tgtacagtca ttaaatcacc taataatcta tttgtaagta
    15661 ctaaagccat tttttttgat aatgcttttc ttgatataca gtttatgttt acaccattca
    15721 aatatatttc accttttatt ggtgatataa gtttagataa ggaccttaat agggtagttt
    15781 ttccagctcc attacttccc aaaagacata gtatttctcc attttttact tctacattta
    15841 tattacttat aacaactttg ttattgtatc ctacacttaa attattagtt ttcaacatgt
    15901 ctaagcccta tccttccttt tcaccaataa cattactaca attggagctc ctaataaaga
    15961 tgttatcgta cttattggta attctactgg tgataaaatt actcttgata ttaaatcaca
    16021 aagagctgta actatactgc ctaaaaatat tactactggt atcaaaactc tattatctga
    16081 tgttttaaat atcatttttg aaatttgtgg cactgcaagt ccaataaacc ctataggtcc
    16141 tgcaaatgct gatactattg ctgctaatat actagatata aatactatca aaactctaaa
    16201 aaatcttata tttatgccaa tactttttgc atactcttct cctagtaaaa aagcatttaa
    16261 aggttttatt atgaatacag tcatcaaaag acctaatata ccaactatta taagtatttc
    16321 taccttttcc catgtaaatc cagaaaaact tcccatagac cacatagtaa attcttttaa
    16381 tttatcacta tttgcaaatg attgtaacac atttgttata ccactacata catatccggt
    16441 cataattcct attattaaaa gagttgttat attctttacc ttatttgcaa ataaaatgac
    16501 tatagacatt acaattaaag ctcctataaa agctgctaca aatatactca ttgaatttac
    16561 actctcaaat cccaatgtgt atcctcccaa aatcatgaga gctactgata aagtagaacc
    16621 tgaagatact cctaatatat acggttcaac tattgggttt ttaaaaaata tctgtaataa
    16681 aatacctgat actgctaggc atgctccacc taccatggtt gcaagtactc tagggattct
    16741 tatattgtat attattttat aatgttctgt attatccata cctcttaata caacatctat
    16801 tacttcttta aattcaattt ttacagagcc aaatgctaca cacactaaaa aagtaagtat
    16861 catacttata attaatacta taaatattgt tgtattcctt attctcattt tattgataaa
    16921 atccatttgt atactcctct tagcaaagct atattaacta aaatctataa aacctatctt
    16981 tttgtgtcct ctctgtacta tttatcataa tttatataat gtttgatttt ttcacctgta
    17041 gtacctgggt ggaacatctc aactaaatct actattaatt cgtccgtctt atctactgat
    17101 tgataataat ctggagcaaa cacccatagg ttcccgtttt ttacaacatc caaatcagcc
    17161 aatacaggtg cactagattt tatagaatct aaatttggtg cataatctga acttgatgag
    17221 tatacaaata tatctgcatc ttttgcaact tcatgcaatt gttcaatact tactcgccct
    17281 tcatttgaat ctattgaagc acatgcattt attccaccag ccatttttat catattatcc
    17341 acatatgaat ctttaggagc tatataagca cttccttcat atacagatgc ccatacaact
    17401 ttaggtttag ctttgttttt tattttttta cttacttctt ctaccttttg tacagtttta
    17461 tttagttcac tagtagccat atcttcttta tcataaaatg cagctattaa acttgtccac
    17521 tccattcttc caaatggatt ttgctcttta tactcattgt ctacaacata attaagccct
    17581 aattctttta atttgtcaat aaattttgta tcagcctctg ataaaccact actcactata
    17641 gttaagtcag gatttaagtc tactattttt tcataatctg gtgctaaatt tgaaccaacg
    17701 taatgtattt ttccatcttt cattaaatct ttaatttctt taatagtcca gtactgctct
    17761 tctgttgtta cggcttttat actatctagt tcatctattg ttctcagaga acaagcctgt
    17821 gttgtagatg ctattaatac attgtccaca ggcgttctaa ctatattagc atttttatat
    17881 ttttcaggaa ttttaatctt ctttgggatt aaaatcagtt ctcgattatc tccatcagtt
    17941 acttttttaa ttccatcttc taaatcttca acactaaatc cactagcata ttttaattct
    18001 aatttcttac cagtactagc ttgtttattc tctttgtcac ttttactaca ccctataaga
    18061 cctcctacca taaaaattac catagcaatt aaaaaaaact gctttaaaac atttgattta
    18121 aatttcatct ctttcactcc aatatcttat tttttatgag tggctaaaaa actttaataa
    18181 tatatgcttt ttaaagtata cttattaaag ttatcctaaa gtcatcttta ataagtataa
    18241 ccttttaata aaataaaaaa cttctcctaa tttaataagg agaagttcat atatcaactt
    18301 tacaaaaacg aattgatact tcattttatt ataaattaca atctccaccc taaattgaac
    18361 ttatataata ctgggcaggt ctcctgactc acggcttttc ctccatcacc cttcccaaaa
    18421 tttttataaa tttcagtgga ttttgatttc gtatccgtat acagtagcgg gggctgtagt
    18481 gaattttcat cactttccct attaaactaa tatagtaccc tagtattatt ttaatttaat
    18541 ttttattaag ataattgtaa tttattgttg acctctttgc aaggttgatt taacatatat
    18601 ttaattcatt catcaatttt ttaatttcac atacttgttt ttcatttggt tttacaaatg
    18661 gagcagtcat ataatcactt atttgtaacc ctctgatatt catagctttt ttcattgctg
    18721 gtataaatgg attggttatt aagtaaaaat ccattgcttt attaatatat ttttgaattt
    18781 ctataacctt ctccatatct ctattattgt aagcttcagc ccatttggag caaacttctg
    18841 gaattaaatt tgataacccc cctatacaac ctgctccccc agacattaga ttatgtataa
    18901 agttttcatc ataaccacta taaaccttaa attctgggaa ctctattttc atagtattta
    18961 ttaaatctct ggtatgtccc atcaaagtta ctgtatcctt atatccaact atgtttttat
    19021 gttttcttgc aagtcttaat gtaagttctg gacttaaatc ataaccagtt ctatctggat
    19081 aattgtacaa atatatttca gcctctgtat tcttagctat ttcactataa aataactcaa
    19141 tactatcatc atcaagagag aaataataag ggcttattat cataacacca tctgctccac
    19201 tattatgtgc atagtttgaa agttctatag attcatttat acacatacga cttgtaccta
    19261 taaacacttt tactctctta tttacatgac tagttactaa atctataagt tctttttgca
    19321 tatccataga catattaaag aactctccag tacttcccat tatgactagt ccatctacac
    19381 ctccatctat aagatggtca tacacttttt tattaccttc tttatctaaa tttccttctt
    19441 catcaaatat tgtaacaact ggtgtcaaaa attctgcttt cataatttaa actcctaaaa
    19501 tttattttaa tatgttcatt aatacatcag atttagcttt aatattaatt atattaaaac
    19561 agttacttaa tgtaattttt taaaattcca atactctcaa tctcacattc aactacatct
    19621 ccactactca tatatcgagg aggtgaaaat cccatcccta ctccagaagg tgttccagta
    19681 gctataatgt ctccagcctc taaggtcata ccttttgaaa gttctgaaac tatgtctgaa
    19741 acgtctgata tgaataatcc tgtattagaa ctttgtcttt cttcaccatt taccctacta
    19801 catactttaa gttttatagg aaaaggaact gcacttttat gtaaaataca tggacctaaa
    19861 gaggtaaatg tatctaatcc ttttcctcta aaccattgaa catgttcttt ttgcaacttt
    19921 ctagcagaaa tatcatttat aacagtgtaa ccaaagatat aatcttctac ctcttctttt
    19981 ctaatattaa cacctgtttt acctattact actccaagtt caacttcata gtctagttga
    20041 ttatttatct caaaatgacc atctatctca tcttctggac ctattgcctt tgtcacacgt
    20101 tttgtaaaat aaaccgtttt ttgtggttct tcaaaactat catcaaagtg agtttgagtt
    20161 tcttcaagat ggtctttata gttcactccc acacataaga tatcatgtat tggtttttct
    20221 ataggaacat gtaccgtaca tctctctctt ttaaaaacat cataattata gaatgtactt
    20281 tcatttataa tagttttcaa cttaataata tcttcactag atacattttg aattaattca
    20341 tttaaatcct taaaatttct actgagattt aaagagttaa catctataat aaataattca
    20401 tctttagaaa aaactccagt tcttttacat tcattttctc cttcagtaaa acttacaaac
    20461 ttcataatct caccctttat taaaattcta tgtatttatt ttcttactta aagattatac
    20521 atagttgcat actctagcta ttgccatttc agtatatcca agggactctg cttttgattg
    20581 ttttccattt gctacatcta caaccatatt taacaaatca tctgttaatt cttccattgt
    20641 ttgtggacca tagataactg gactagcatc aaaatccata ttatcttcca tatttgcaaa
    20701 agttatttta tttccagtta tttttattac tggtgctatt ggatttcctg atggtgttcc
    20761 tcttcctgta ctaaatacca ctatttgtgc tcctcctgct accataccag caacagatga
    20821 agcatcattt ccaggggtat ccattataac aagacctttc ttatcaattt gtttggcata
    20881 atcatatact tcacttattt gtctatgacc acctttatgt atacaaccaa gtgatttttc
    20941 ttctaaactt gttaaaccac cagctatatt tccaggagat ggattacctt ctctaacttc
    21001 ttctccaacc aattttaatg aattttcata tctatgtact atatgtaata ttttttcttt
    21061 tacttcttca tttactgctc tcctagctag tatatgctct gcacctataa attcagtagt
    21121 ttcacttaaa attgatgttg ctcctaaatc gacaagttta tcacttaatt cacctattaa
    21181 tggatttgct gctaatccac ttgttgggtc agacccacca cattcagttc ctagtataag
    21241 ttcagatatt ggaaattctt cttttctaag caaagaagct tcttgtgcca tttctctagc
    21301 ataacgaaca gctttttcta tagtttttaa agttccatgt tcttcttgta ttacaagagt
    21361 ttttattggc ttatttgttc tctcttttat tgcatcaact accaaatcat tttggcaatt
    21421 ttcgcatccc aaagatacaa ctattacacc atatacattt ggatttgctg cataaccagc
    21481 cataacatcc atagttaatt gttggtctga atgtacttgt gaacatccat tctgattatt
    21541 aaaggtaact gccccttgaa cttgtgatgc tattattcta gttgtatctg atgcacacac
    21601 actcgtaggc aatattaata tgtggtttct tataccaact cttccatctg gtcttttata
    21661 tccataaaat ttcatactga tttcctccta aaatagtcta tatactttct ataattttta
    21721 tatattataa tttttattac actcactaat caaacagcta aaaacctatt taaaaactat
    21781 ttgacactaa aagcaatgaa tacaactttt ataactctat aaatattatt ttatatatta
    21841 gtcataactt gtttatccaa atcctctcta acacttctaa cattatgaat atgtacatgt
    21901 tggccacttt ttatttcctg atttgcttct cctatatgtt caccatattt agtaacttta
    21961 tcacctttgg ccatatcttt tatagccaat ttatgataaa tagttatatc atctagtgct
    22021 gtaatcgttt ttactgtttt gtcttttaat ttaaaactaa tttcactacc tttagctatt
    22081 gcttcaatag ctactgcaac attatctttt tcatcaatta tcactgcatt tatcattatt
    22141 cttgctctcc tttataaata atataatttt attaatatcc taaaatctaa ttaaagaatt
    22201 tattatttta ccaacaactc actgtaatat aaattaagct gttattgtac catcctcatt
    22261 ttgttttggg aacttaggac atccatactt cttagcccac caactagtta taagaggtac
    22321 taatattgca gtgacaacta ctgcggctgc cacttgtgtt gtagctgttg caacatatgg
    22381 ttgccatgat gggtcaacta atgcaacagc agcaggtaca gcaattgcat ttccagcagt
    22441 tgtagctaca gcccatcctg catatcctgg acgttttcct ataaatttat cacagaatac
    22501 tataaatata cctcctacaa atacagatat taatccaagt aatactcctg ataaacctcc
    22561 ttttagtata cttgcaagat ttatgctact accaagtgca aaccctacaa aaggtacgag
    22621 taaattacac cctggctcta aaaactcttt catttttttg tcaaaatttc ctattatcat
    22681 accaacaagt attggcacta ttgcagccaa taatgacaac aatggtacat ttgcaagacc
    22741 tgaagtccca agagctatta atgtaaaaaa tggtccatca tttaaagtaa gtaaactcat
    22801 agttgcacaa tctgtttcat ctccatatga agtcatcaat gaaagatata tactaccatt
    22861 actatttgta actgcactta taacagccaa tgttgtcaga ccaaggacac catccattcc
    22921 aaatagcttt cctataagta tacctatcaa agcacctata gcaaatttag aaccaagaag
    22981 tataccacct ctctttaaaa cttttggcat ttctctaaat tgaagctgag ttcccaagca
    23041 taccagttga accccaatta tagttgcaga cccaacactt gaaaatactg ctgtcgtaaa
    23101 cgaacctatt tgtacaatat ctgggcaaaa ggtattaatg aaagctgcta taaacattgg
    23161 cactatcatc atacctgcag gaattttttt aataaaatcc attatcataa agttaatcct
    23221 cctaaaacat gatatttaat tactcttatt aagtttttag ctttaattct aacggccgtt
    23281 tcggttcgta tttaagaact aagtttttaa ttacgaatct ttgaattaat gatagcattt
    23341 tcctatttat gagtcaataa aaaaaataaa tttttgtcaa aattttttca aactttctga
    23401 taataccttg tatattatca aatgacttcc ttatatgcaa aaatacagcg tatttaaaat
    23461 ta
//
LOCUS       DAEMGG010000055        22630 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEMGG010000055 DAEMGG010000000
VERSION     DAEMGG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.055"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(614..754)
                     /locus_tag="KPK24_002019"
     CDS             complement(614..754)
                     /locus_tag="KPK24_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299691.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(1012..2172)
                     /locus_tag="KPK24_002020"
     CDS             complement(1012..2172)
                     /locus_tag="KPK24_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG2299692.1"
                     /translation="MKLNKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGSVSAVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSISDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(2343..2546)
                     /locus_tag="KPK24_002021"
     CDS             complement(2343..2546)
                     /locus_tag="KPK24_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG2299693.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(2633..3667)
                     /locus_tag="KPK24_002022"
     CDS             complement(2633..3667)
                     /locus_tag="KPK24_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG2299694.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTATSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKEAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSKFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            complement(4189..5550)
                     /gene="rlmD"
                     /locus_tag="KPK24_002023"
     CDS             complement(4189..5550)
                     /gene="rlmD"
                     /locus_tag="KPK24_002023"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG2299695.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIIVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(5577..6299)
                     /locus_tag="KPK24_002024"
     CDS             complement(5577..6299)
                     /locus_tag="KPK24_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG2299696.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(6299..6628)
                     /locus_tag="KPK24_002025"
     CDS             complement(6299..6628)
                     /locus_tag="KPK24_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG2299697.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            6958..7884
                     /gene="fba"
                     /locus_tag="KPK24_002026"
     CDS             6958..7884
                     /gene="fba"
                     /locus_tag="KPK24_002026"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG2299698.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(8019..9650)
                     /locus_tag="KPK24_002027"
     CDS             complement(8019..9650)
                     /locus_tag="KPK24_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2299699.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10133..11170)
                     /locus_tag="KPK24_002028"
     CDS             complement(10133..11170)
                     /locus_tag="KPK24_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG2299700.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(11189..11974)
                     /locus_tag="KPK24_002029"
     CDS             complement(11189..11974)
                     /locus_tag="KPK24_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2299701.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(12007..13140)
                     /locus_tag="KPK24_002030"
     CDS             complement(12007..13140)
                     /locus_tag="KPK24_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG2299702.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(13188..14315)
                     /gene="hadC"
                     /locus_tag="KPK24_002031"
     CDS             complement(13188..14315)
                     /gene="hadC"
                     /locus_tag="KPK24_002031"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG2299703.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(14315..15541)
                     /gene="hadB"
                     /locus_tag="KPK24_002032"
     CDS             complement(14315..15541)
                     /gene="hadB"
                     /locus_tag="KPK24_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG2299704.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(15556..16356)
                     /gene="hadI"
                     /locus_tag="KPK24_002033"
     CDS             complement(15556..16356)
                     /gene="hadI"
                     /locus_tag="KPK24_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG2299705.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(16395..17594)
                     /gene="hadA"
                     /locus_tag="KPK24_002034"
     CDS             complement(16395..17594)
                     /gene="hadA"
                     /locus_tag="KPK24_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG2299706.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            18271..19269
                     /locus_tag="KPK24_002035"
     CDS             18271..19269
                     /locus_tag="KPK24_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG2299707.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            19631..20158
                     /locus_tag="KPK24_002036"
     CDS             19631..20158
                     /locus_tag="KPK24_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG2299708.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            20185..20781
                     /locus_tag="KPK24_002037"
     CDS             20185..20781
                     /locus_tag="KPK24_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG2299709.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(20846..22429)
                     /gene="asnB"
                     /locus_tag="KPK24_002038"
     CDS             complement(20846..22429)
                     /gene="asnB"
                     /locus_tag="KPK24_002038"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG2299710.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         7043 a         4078 c         2698 g         8811 t
ORIGIN      
        1 aatctataga aattgtggta ttaatgttta aataaccaac tttttctaaa ataaccttta
       61 taggaatata tgagttcata agtaaagata gcgaaataag aaaccagttt ggtatgtaaa
      121 atctagattt ttaattaaat tatcaagatt atatattata gtatcaaaaa tagaaaatta
      181 aaattaaatt ttttataata aattgtatac attataaatt atattaaatt tttaatttaa
      241 gtaatagtat atgtaatctt tagtcatctt cttaccagtt ttttatccac ttttctaatg
      301 aagttatttt ttcatatgtt aattcctctt gaaataatga tactaaaaga ctttttataa
      361 aattataact taaaagttta tgtatttttt tgatatgaat ttatagtact tatcttcttt
      421 aattgagatt gtgtatattt aactaaacat tacaacaata attctatgaa atcaaatcaa
      481 ttatttacat atttttttag aacaataata agtatatatt cacaaaattt tgtgttaata
      541 tttttaattt gtgatataat aaaagcaagg aaacatattt tacttaactt tgagtgatgt
      601 ttccttcaac aaattatttt gaagatttac ttttctggaa ctttatatta agttcccagc
      661 cactcttagt tggtcgctca gagtggctct ttaatttatc gtaaatgtaa ctggctataa
      721 caccagttat aatactcatc attagatttt ccatagtttt cacctcctct cataagaaag
      781 tgaagaaact tccatggaaa caccactcat agctttttca aatatttatt ccttgccata
      841 ttaattatat cataaatatt ggaaacataa aattttaata cttatcgtct ttattattat
      901 tttttataat taataatttt ttttaataat acataatata aatttatatt tcttcaataa
      961 aaaagacaat cttaattaat tgtctttttt aatatatatt tggctaatac attattttac
     1021 acaaacatta caagggtcta ttttaccttg tgacttagct tgttttaatg ttactttatt
     1081 tattgtttta cttcttttta gtgtagaaca attttttgtt agatgatatg attttccatt
     1141 aggagttaca tatactgtac ttttatcagt taatccagga ttattagagc taggctcatt
     1201 acttggtttg ttcgtattat ttgaattagt atcttttaca atgcctgtat ttgaatcccc
     1261 aacattatta gttatattta aacattgttt tataacaaca tcatcaatat ttccaatagc
     1321 agttatttta cttgcatttt ttaatatact cttattactt ccatttccaa ctaacactat
     1381 aggtgttttt tgtgcaattg tagaaccaac taaagaataa actaaatcag tcccacttgc
     1441 tatgtaaaat tcttttgagc cattataaaa tttatttatt atttgtttat ttgtatcgta
     1501 tctatctact ccacctattc ttgttgcttt agtttcattt attaacttat cactaatact
     1561 gacttttcca ccaattgcat aagtttctaa tccatctgta gcaaaacttg tagtgtttcc
     1621 atcagttaat actattggag atttatctct aactgctata gaagcaacac tcatagcatc
     1681 tggttcacct ttaaaagcat tagttaatat tactttttta acagcactta cacttcctat
     1741 ttcttttgca actttatgac ttgtatctat tctatcagtt ccacttattc tatttatgct
     1801 tattttttta tcacttaata ttttctgaac tgatgctcct atcgaatttt ttcctccgat
     1861 tagatatact tcttttacct tttctaatct ttttaatgtt tctgaaggga ttgtatcttt
     1921 cttagttaac aatattgggg catttttagc tcctgacaat ccacttgctg acaatccatc
     1981 agataatcca ttttcagaat taataagtat tgcaatatca taattttgtt tatctgctat
     2041 taaacctgct gtttcatact tatttgtacc ttttattgaa ttaatagaac taagagcgtt
     2101 tgatgttgga caactagcaa tcaataatga aacagacatc cctattgcca atagtgtttt
     2161 gtttaatttc ataatgaaat cctcctataa attttataat attacttaaa tattataatt
     2221 ctacataaaa gaatattttc ctttatgaaa ttagtttata tttcaaaatt ttgtcacttt
     2281 ttaaatctat atactaaaaa atagactatt tgtaacagtc tatttcttag tatatcaaat
     2341 atttatttag gtttacgttt ctttgtacta gtttttgtat atgatattcc agttccaggt
     2401 atactcaatg tagctgattt tcttcctttt gaattaactg acactctagc acctttaaca
     2461 ccagcactta tacctacact cttcttacct aaatttaatt tgagtcctcc tcctagattt
     2521 atgcttttcc taaagttaaa tcccatagat ataaccttcc cttctatatt ttaaatattt
     2581 atacttaaaa ttatatttgt tatacacttg ccatcattgg tatatctagc atctatatct
     2641 ttttacgttt tttcttctta tatactactc cagcaactac agctgctgtt ccagctccaa
     2701 ccccataagt agcatttcta cttttctctt cttctctttt ttctaaacct tcatcatata
     2761 caccttttaa cttagtttca atagacttca tttcatctgg tatactaaaa tcttcttcat
     2821 tgtaaccttg attatataca ctttcttttg ctttaggtaa cttaacctta ccattatcat
     2881 atccttcttt aaaccaatta gcatatttaa cattacctat agttgatgtg tcaaatcttt
     2941 ctcctttgat agcactctca aatccttgag tagtgtaatc attttttaat tgttcttgta
     3001 tttcgtcata agcattatta taagcatcta taaatttaga atctatgttt tcttcaaatg
     3061 tcattttttc tttatcttta ttgctatcct ctatcccttt agctttatat tcttctatct
     3121 ttttagtctt atattcattg tatccagttg aatatccggt atcataagca ttttttaaaa
     3181 cttctttttc atatgtatta ttagatttag cacctgtaag ccctagatta tatccagtct
     3241 ctttagcctc cttttcttcc tcttctaatt tagccttacc tttttcatat ccttctgagt
     3301 actttgattc atatgtatca ctataatctc cactataatt tgatgaacta taatttcctt
     3361 tatacccatc ttcataccct tgattatatc ctttttctcc aacagacctc ttttgctttt
     3421 cttcattatt aaccgaagtt gctgttccag aactgcttcc tcctccagta taaggacaac
     3481 caccattatt atgtaaatga gctggataac caccacaatg atagtgataa cttcctaatc
     3541 cagatttatt tttattatca cgatgacctc ctgatgaatc agttcttcct gaatgagcat
     3601 acgaaaagct ccctacacta atcatagtta tactcattgc aacagctatt aactttttct
     3661 taaacataat ctatttcccc ctaaaatatt ctatgatttt attatatcag tcaatatatt
     3721 atgtttaaac cgttaaaatt cgacaatgta aatatttgat gaattgtatt tgtaaataaa
     3781 tcttttagca tatcttgaat atctgtagtt gttttgacat catattcttc aataagtttt
     3841 ttaatatttc gtttttgtta aacataaaaa gcgtgcactt ttctttgtat agtgatattt
     3901 gatattttaa ttatcactac tttagaagaa tacacacttt attttaaaaa ccctgatact
     3961 atgttattag aaaatggtac taatataaaa gatatccagc atatactcgg acatgctaaa
     4021 acttcaacaa ctatggatac atatttacat gtaactaaca acatgagaaa ccagacggta
     4081 aatatttttg aaatcattgt agaataaatt tgccacccaa aaataattat ggtggcaaat
     4141 aggtggcaaa tctactttta agacacacta taaaagtctg aaaccctgtt attctctaat
     4201 aagtttaact acagtctcac aatgtggcgt atttgggaac atatccatac acttaaccat
     4261 cttaatctca taaccatacc ccttaaaatc aaccaaatca acaaccaaag acttaggatt
     4321 acaagaaata taaacaatct ccttagcacc aaatccacaa atatccctaa tagcatcctt
     4381 atgaatgcct ggtctaggag ggtcaacaat aatcaaatca ggcttgtcct ttaaatcctt
     4441 aacaacctta gccacatccc cagcaataaa ctcacaatta gtaagtccat tcaacttagc
     4501 attctcatta gcagcaacaa cagcttcctc aactatctca ataccataca ctttcttagc
     4561 agcaccagcc ataacctgtc caatagaccc agtaccacta tacaagtcaa atacaacttt
     4621 atcattgtaa tctccaataa aatccctagc aatactataa agaacctcag ctcccttagt
     4681 attggtttgg aaaaacgaaa atggtgaaat cttaaactta agacctaaaa tttcttcttg
     4741 tatataatct cttccataca atactcttaa ctcatcacat tggactgcat ctgctaaacc
     4801 atcattaata gtatgaagta cacctactaa ctcagctttt aaatcaatcc ctaaaagcat
     4861 atctttaaac tcatacatat caaaatcttc ttgagaagaa gttactatat taactataat
     4921 ctcattagtg tgaatccctt ttctaactac aagatgtctc aaatatcctt tgtgattcat
     4981 tgttctataa taaggaagct ttttttcatt gaaaaactct acactagaaa ctaatatctt
     5041 tgagaagtct tcatcaacca acatacactc ctcaacagtt tgtatatcta tatgctttcc
     5101 ttttttatga agacctaatg tcaaaggtcc attttttact tcatctccaa aagtatattc
     5161 catcttattt ctataatatt tgttttctgg acttccttct atccctaaaa actgaaatcc
     5221 aaataaatct tgctttaaaa ataaatccat aacttgtttt tcttttattt ctaattgttt
     5281 ttcatatggc acagacaata gtgtacatcc tccacactct ctaaagtgct tgcaagtttc
     5341 ttcagtttca agtggtgagt tctctaataa ttctatcatc ttaacttcag ctttcttact
     5401 tctaactttt ttaactccag ctctcacctt ttgaccactt attccaccct tcatatggat
     5461 aactctatct ccaacttggc ttaaagatgt accaccaaat tccattttat ctacttcaaa
     5521 ttctataaca tcttttttct tcacagacat tcctccaatt tactatagta tctttattat
     5581 ctttcttcaa tcaattttac ttcttcatct gttaactctc tatactcacc taactctaaa
     5641 ctttcatcca attttaaatt gcccatagac aatcttttta agtatactac ttttttgcct
     5701 acgctttcaa acattctttt tacttggtga aacttacctt catgtattgt aagctctatt
     5761 tctgattcat catcactttt taatatattt aactgtgatg gcatagtttt atatccgtca
     5821 tcaagcacta caccttctaa aaaagcttct acatcttctt cagtaactac tccatcaatt
     5881 tttgcataat aagtcttagg cacatgcttc ttaggagata aaactctatg agaaagcttt
     5941 ccatcattag ttaaaactaa taatccctct gtgtctttat ctaaccttcc aacaggaaat
     6001 ggttcaaaag ctaagtatga taaatcaatt aaatctaata ctgttgggtc atatttatct
     6061 gtagttgctg atatatatcc atcaggttta ttcatcatca aatatatata ttctctatat
     6121 ataacttctt ctccattaaa taatatctca tcattttcag tgtctacttg agttcctgga
     6181 tttgaaactt cacttccatt tactactaca gacccttgtt tacaatactt ctttatttca
     6241 cttctgcttc cataacctaa atttgaaaga attttatcta tacgttgctt tttagccatt
     6301 aaaaatgctc tccattctca taatattttt ctttttcaaa tactgtaaat gctaaatcca
     6361 tactttctac atctaaatta tttcttatac tatcagttat tattcttgct actatatctt
     6421 gtacttcttg tcctctatca aaccaagcta cttcaacaaa aggatataca tctgctattt
     6481 taccatctct tattgcaact gactttatac attctatttc aaagtagtct cttggacatt
     6541 ttacagcttc aactaaatca tttatcattt tttcacttat tttacatata tcattttcat
     6601 taattcctct tatttttatc tgtggcatag tttatctcct ttattttaaa tttttagtga
     6661 attatcatcc acttaattcc cttcgataac gtttgaagtg gtaacttctt ggtcaagaca
     6721 actattgtta tcagtatctc caaactaata aggtagttcc tacccttata gttagcatta
     6781 tatagctaat tttcctaaat tgtttatatt ctttattatt ttatttaatc ctattgtctt
     6841 ctatttaaca ttatacttgt aatttatagt tattacaatt tactatatac tttttttgat
     6901 ttaacgttat aatataagtg atgggtaaat tttaaaagtt aataggagga gtttaaaatg
     6961 gcattaatta ctacaaaaga aatgtttaaa aaagcttacg aaggtggatt tgctataggt
     7021 gcatttaaca taagtgactt agaacaatta caaggtgttt taaaagctgc taaagctaaa
     7081 aattcttatg ttatgataca agcttctatg tcagctgtta aatatgctgg acctcataca
     7141 ttagttgaaa tggttaaggc tgcttctgat gaaataggtg tagatgtagc tcttcactta
     7201 gaccatggtc caaatatgga tgctattaaa acttgtattg atgctggttt ctcttcagtt
     7261 atgatagatg gttctcattt tgactttgaa gaaaatgtta gaataactaa agaagctgtt
     7321 gaatatgcac actctaaagg tgttgttgtt gaagcagaac ttggtgttct agctggaaca
     7381 gaagatgatg ttacttctga tgttcacaag tatactcaac cagctgaggc agttgagttc
     7441 gttgaaagaa caggtgttga ttcattagca atagctattg gaacttctca tggtgctttc
     7501 aaattcaaag gagaagctaa attaagattc gatatactag aagaaatcca aagcaaatta
     7561 ccaggtttcc caatagttct tcatggtgca tctgctgttg accaaaacgc tgttgctaca
     7621 tgtaatgaat ttggtggaaa tatagctggt gctaaaggtg tacctgtaga tatgttaaga
     7681 aaagcatctt ctatggctgt ttgtaaaata aacatggata ctgacttaag actagctatg
     7741 actgctgcta tcagaaaatt cttagctgaa aatccaaaag aatttgaccc aagaaaatac
     7801 attggtgctg gtagagatgc tatacaagct gtagttgaaa gcaagataga tgatgtttta
     7861 ggttctgcta actctataaa ctaatataac ttttaaatat ttataaaaga aaagagatga
     7921 cttaaagtaa ttaaatttat ttttaagcca tctcttttaa gttgtataaa ttatgtatct
     7981 tataatattt taattatatt gtctttttaa actgacagct aaacgtgaat atgtccactc
     8041 tttatctttc tatggatggt agaaggatat atacctataa cttcagcagc ctttgttatt
     8101 gaaccatact tatttatagc cttacttatc attatttggt ccactatttt atatgcatca
     8161 ttaagattca taattccatt taccactatt ggtgataaat catctgtttc attatcaatc
     8221 atatctaaat ttatcagcat gttaaattca tcttctccaa ttacattttt agctgataat
     8281 actacaaatc tttcaatgat gttttttaat tctcttatgt ttcctggcca agaatattct
     8341 tctaataact caataacttt aggcgaaact tctacttctc tattgtactt ttcattgtaa
     8401 agttttaaga aataatttac taatggtact atatcctctt ttctttctct aagaggaggt
     8461 atttttactg gaataacact taatctatag tacaaatctt gtctaaattt aaggttattt
     8521 ctaaggtctt catgagaaat attagtagca cttatatatc taacatctat ttttataggc
     8581 ttactaccac ctattctagt aatagtattt tcttgtataa ccctaagtaa ttttttttgc
     8641 atctgcattg gtaactcacc tatctcatcc aagaaaatag ttccaccatt cgcctcttca
     8701 aaaagtccct ttttaccttt tttcttagct ccagtaaaag aaccttcttc atatccaaaa
     8761 aactctgact ctaataattc atttggtata gctccacagt ttatagctat aaagggtttg
     8821 tctctccttg aactatactt atatattgtt cttgctatta tttcttttcc tacacctgat
     8881 tctccattta taaatataga agaatctgag gctgcagctc tcttaacaac tttttttata
     8941 ttgtccataa tcttactttg acttatatac tctgtgtctt ctgatatatc actatatttt
     9001 atctgagatt caagcttaat aacccttgaa atatctctcc ctgttagtac tcctcctgta
     9061 agctttccct tagaatccca tcttggaact gccgtatatg caataagctt cccatctggg
     9121 taaacgatat ttctataaat ctttttcttc actttaagca tgtttaatat tattggttct
     9181 tcaactaatc cttgtctcac aaggtctgtt atatttctac caagtacctc agttctttta
     9241 aaaggtaata tctcatcact gacagtgttc atcttttcaa ttaccccatc tttgttaaac
     9301 acaacaacaa cttcatctat atgctctatt atatcttcaa ttactttatc agatattaga
     9361 gacttctcaa ataaataaac tatattattt tcattattat tatctttcat attgtatata
     9421 tgtgcagtca tccttaactc tctgaaagct attttctttt tactatctgt cagtatttct
     9481 gttttgtcaa aatttgaatc taattcattt atatttctag gcttttttat atctagaaaa
     9541 ctaaaaatac tatctattag atgattacag tatattattt cgcctgattc tttacataaa
     9601 atggctggat tttctacatg ttctataagc cctattaaat tattatccat taatattcgc
     9661 acctttcatt aagtaattta ttgaactaca tacaaaattt attgtttgct tacaataaat
     9721 accatcagca catataatca actccttata atattcgtct tttcaagtag tttatttcat
     9781 taaaacgtaa ctaagatata attttaatga ttatagtttg ttaaaaattt cattttgctt
     9841 ttaattgtat tttatcacat atttagtaat tatttgtcta tttaatacaa tttaattaaa
     9901 tttaagattt atttaaaaaa acaaagataa tccttacaga atataactca caatttgtaa
     9961 gcaatataca tataaggatt atccagatta tcttgtatta taaaatttta ttcaaacaga
    10021 ctttttaata actgacaaat acacactagc aataacttat caaagggtta ataattatgt
    10081 tactaactaa taagacttat tcaatcagta atatttatat agtttgtatc tattaagctt
    10141 ttatagcttc taataaatca tcaacgctat ctaatttttc tataagcatt ggaactattt
    10201 catgtaagtc tccaactact ccatagtcag cgatttctaa aattggagct gctgggtttt
    10261 tgtttatagc aattatgaaa tctgatgatt gcatacctgc taaatgttgt attgctcctg
    10321 atataccaca agctatatat aggtttggtt taacagtagt tcctgtttga cctacttggt
    10381 gagaatggtc tatccatcca gcatcaacag ccgcacgaga agaaccaact actccaccta
    10441 atttgtcagc taatttctta agcatttcaa atccttctgg tccacctaat cctaaaccac
    10501 ctgatactat aacatttgca tctgtaagag atactaaatc tttcttagtt ttaactgttt
    10561 ctaaaacagt agttcttata tcatcttgag ttattttgta gtctaatgct ataacttcac
    10621 cagttcttgt ttcgtctttt tcagctttat ccataacacc aggtctaact gtagacattt
    10681 gaggtctatg gtttggacaa atgattgtag ccattatgtt tccaccaaat gctggacgag
    10741 tttgttttat tttcttatct tctgggtcta tttctaactt agtacagtca gctgttaatc
    10801 cagttccaac tcttgaagct attctaggtg ctaagtctct acctatatga gttgctccga
    10861 aaagcattat ttctggtttt atttcatcta ttgcatcttt tattactttt gtatatgcat
    10921 cagttgtata tttttctaaa agagcatcat ctgcaacata aactttgtca gctccaaaag
    10981 tgattaattc tttagctaat ccatcaacat cttttcctaa taatattgca caaagttctg
    11041 catctacttc tttagctaat tttcttcctt ctcctaataa ttctataact actggagcta
    11101 tttttccttc tctttgttct gcaaatatcc atacgttttt ataagaactt aaatctttta
    11161 tatcattcat agtttgtact cctccaactt atataatgtg cttttgtttt agaccgatta
    11221 ttattttact agctatttca tcagctttac cttctaagat ttctcctttt cctcttggtt
    11281 caggagtaaa tgatctaaat actttagttg gtgatgcttt taaaccaact tcttctttat
    11341 ttacatctaa atcatcaatt gtccaaactt ttacatcatg tttatatgct tttactattt
    11401 tatcaacaga catgtatcta ggtgtattta actcttttac tgctgttaaa agtacaggtg
    11461 tacttacttt tattaattcg tatccatctt ctaattgtct ttgaactatt atatcttttc
    11521 cttctatttt gaaatcttgt acatatgtaa cttgaggaat gtctaatttc tctgcaattt
    11581 gtggtccaac ttgtgcagta tctccatcta ttgcttgtct tcctgcaaat attatgtcat
    11641 agtcacctac tttagatatt cccgctgcaa tagtatttga tgtagcccaa gtatcagctc
    11701 ctccaaatgc tctatcactt aataatattg catcatcagc acccatagct aaacactctc
    11761 ttagcataaa gtctgcttga ggaggtccca ttgttataac tgttatagtt acatcatcat
    11821 gctcatcttt aatttttaaa gcttcctcta atgcatttgc atcatctggg tttaatatac
    11881 ttggaactcc atctcttata agagtacctg tttctttatt tattcttact tcgttagtat
    11941 ctggaacttg ttttacgcaa actaatattt tcaaagtcta tacctccaat ttcattagat
    12001 tatttattat tttaatacgt ttgatgaaat taccattttt tgaacttctg atgttccttc
    12061 gtatattgat acgattctag catctctgta cattctttcg atttcataat cttttataaa
    12121 tccatatcca ccgtgaagtt gtaatgcttt ataagcaact tcatttgcaa tttctgatgc
    12181 atagtattta gccatagcag attcttgagc aactctcatt cctgcatctc tcttttgtgc
    12241 tgcatcataa actaatcctc tagctgcaca aacttttgtt tccatgtcag ctatagtaaa
    12301 ttgagtattt tggaatttag ctataggctt accaaattga actctttgtt ttgtatattt
    12361 aacagcttca tctaaagcac cttgagctat tcctagagct aaggcagcaa cacctattct
    12421 accaacttct agagtaccca ttgctatctt aaatccttga ccttcttttc caagtaagtt
    12481 ttcttttgga actttaacgt tttcaaatat taagtcagaa gtttcagttc ctcttatacc
    12541 catcttatct tcatgagctc ctgttgagaa accatcccat ttactttcta ctataaacgc
    12601 tgatgttcct ctaagtcctt tacttctatc tgtaactgct attactatag ctacatcaca
    12661 gaatggtcca ttagttatga aagtttttct accatttaat atatagtggt ctccaactaa
    12721 ttctgctgtt gtttgttgtc cacctgcatc agaaccagct cctggctcag ttaaaccaaa
    12781 tgctcctatt aattcacctg ttgctatacc ttttaagtat ttttgttttt gctcttcagt
    12841 accattgtaa agtattggta atgaagcaag agaagaacca gctgtaacaa aagtagctgt
    12901 tgaagcacat cttctagcta tttcttccat tactatagcg taagaaacat atcctgctcc
    12961 cccaccacca tattcttctg ggatattact gcttataaat tttgcatcag ctaatttttt
    13021 gattaattcc ttaggcatta cacctgtttt atccatttct gctggtaaag tatctaattc
    13081 tttactaaca aaatctctta ctgcttttct taaaagttct tgttctttgt tatataacat
    13141 agcttcctcc aattttcata ttttatagta aatttatttt aatgaactta cgctaaactc
    13201 atcatctcag caaaagtttg aatacgagtt ctggcttgtt cattattctg agtttgttgg
    13261 tcgatttcaa catataaagt aggtattcca ctatcttcta tatcttttct aactaaagga
    13321 taatcgtatt cttctgggtc acagaatttc atcatacaga agataacacc atctatatct
    13381 ttctttttaa cttcatctac tataagtgac ccacgttttt tcttagggtc ataagctaat
    13441 gaacatcctt ctatgtttga ccattgtctt gctaatctct ctaacgcatc atcacctgct
    13501 ggtacatctg ttctgaattg tcttgtttct tgtgctaagt cgtcagctac aactgatata
    13561 ttgttgtctt ctaaaatgtc taatatatct ttagaatcag ctaatatacc tgttaataaa
    13621 actttctttc cagaacagac ttcttctggc atagcattta attttgctat taaatctttc
    13681 actagctcag tatgttcttc ttttctcata aagaacccac tcttaattac taagcttctt
    13741 attgatggtt ttatagtatt agaatgttta taagctactt caacaaagtc tctcatagtt
    13801 tttctatgtt cattgtaaac ttctatactt tcatgaattt ttgcttcttc tatttcatat
    13861 ccacaaattt cttcaagttc tctttttaca cctttgtact cactgattaa gtattttaca
    13921 ccagcttcaa gttttctatt ttgtgggtgt actaatgata tatattttat atgaggtact
    13981 gctgatttcc agttttgtcc taaacaaatt agtgtatcac acatacctgg tataataact
    14041 ccagataatt catcataagc accttttaat ccatattcta aacatgattg cattattgaa
    14101 catgcaaatg cagggaaata ttgtttagct aaatctaatt ctgtttgtcc tccccatata
    14161 ccaactggaa gcattccagc tgcatgtata atttcttctg ggcaataaac tgggaaacaa
    14221 cctatagctt ttttaccagt ttcactttta tattttttta cagccgcatt tggattttca
    14281 actacttctt tcattttaga taaaatagct tccattatat ctcacctcta ttaagttttt
    14341 ttctttcttc cattacttca actaaacctt gaattcttgt ttcaaattga gcattagtga
    14401 aagctctagg gtctgcttgg tcaccatcaa atccagcata tggtaatcca gtttcttcag
    14461 ctgctcttct ttgcatttca tattgtatta aactcataag tttacagctt ctgttcatat
    14521 gatagaatgc tccatcacat ttaccctcta ctaaagaatc aactctatat tttgtcatac
    14581 ggtctaggtt tacattgtta aacatagtac tatatgctac tgccattcca tctaaatcat
    14641 taacttcata ttgtaatgcc caagcatgtg ggtaaacact acctgtcatg ttaactccaa
    14701 atttagctaa tgttttcatc ttgtatccta tatatggcca acaaggtata ccttccatca
    14761 ttattctgta tttttcttcc cctctgaaag aagatttacc agttttcatg ttgtcctcta
    14821 attcttctat aagtaactta aatgcttctg tagtttcttt tttacctcta gcacaaacta
    14881 ttacagccat gtaagtaaat aagtcaaatc cattcattgg agaaggagaa gaatctgctg
    14941 gtaaactcat agaatacttc catagtcttc cgttttcagc tgatattttc attacttctt
    15001 caaacttctt agggtcaaat ttctttcctg atataatttc tagttgtttt atagcttctt
    15061 caaattgagc tttaatataa tctattcttg attgagtaac ttcgtcttca ttattgaaag
    15121 ttgtatcaat cattattaaa ggtatatcta attctcttga aatattttca taccatttta
    15181 taacttggtt acatatattg ttacagcaaa gtaggaaatc tggagccggc atatccaaag
    15241 cttcacatcc accattttct aaaagaccaa aatttgttct tgcatatgca cataggtcaa
    15301 tagaatatcc tttagattca gctatttcac ataattctaa agaacctttt ttagctgcta
    15361 ctccagctgc ttggttttct ggatataata cgtttaagtc aaatacttct gctaactctt
    15421 gaggaaatac tgatgttgac caacctacag gtcttccttc ttctttagct ttaaatgcat
    15481 ttgcatattg ttcagctaat aaatcattaa ttactactct agcttctttt ttttcagaca
    15541 ttttcatccc tctttttata tatttttcac ttctttttgt gattcttttg cttcatcaaa
    15601 agcatataac gctgctccta aagcaccagt taattgaggt atatcaggta caataatttc
    15661 tgtgttgatt tctcttgcca tagctcttac aataccacta tttctagcaa ctccaccaac
    15721 cataactaca tttctttgta ctcctattct ttttactagg ctagaaactc tctttgctac
    15781 tgaagtatga atacctgcca ctatatcttc aatttttgca ttttcagata aatgtgatat
    15841 aacctcagac tctgcaaata ctgtacatgt actgcttatt gatacttcat tttgagaatt
    15901 catagatata cttccgagtt cagatacatc aacctctatt atttttgcca ttacatctaa
    15961 aaatcttcct gtacctgcag cacatttgtc attcataaga aagtttaata gtcttccatt
    16021 attatctaat tttaatacct ttgcatcttg accacctatg tcaataatgg ttcttgtctc
    16081 tggaattatg aaatttaccc ctctagcatg acagcttaat tcacttattt gcttatcagc
    16141 atctgaatag ttcattcttc catatcctgt aactacaact tttttaatat cttctcttgc
    16201 aagacctgtc ttgccatata atttttctaa aactcttgat ggtccagtag tcccagtacc
    16261 agaggatatt gtttcagaag ctacaatatc ttccccattc tttaagatta ctccctttga
    16321 tgcagttgaa cctatatcta atcccattgt gtacatcttt agcattctcc ttttaaaaaa
    16381 tctagaatta ctaattaata tcttacaact ttactatctt taaagttatt tattttctct
    16441 tcatcgtagc ctaaagattt taatacttct acagtatgtt gacctacttt tggtgctggt
    16501 gtatattctt taattccttc atttctaaac ataactggag tattaactaa gacacctgta
    16561 tttccgctat cgtatgtttt cttaaataag aagtcatttg cccaagcttg ttcgtcatct
    16621 aataaatctt cacagctttg aattttttca aatggtaagt ctgcttcttc taataaagct
    16681 gaccactcgt ctaatgtttt ttctaacata gcttctccaa ctatcttaac taaatcttca
    16741 acatgattaa ccattgaatc tatgttatta tatctatcgt cttctaatat atattctcta
    16801 tttataacct tacagaattt gcctaaccac ttgttgtatt gtattaaagc taattgaatc
    16861 catcttccat ctttacattt atatgtagtc attaatgggc tgtttggatt ttctcttgat
    16921 aaaggcattt catttccgta ttgtgctgtt gttatcattg ttcccattcc atatatagct
    16981 gtatggaaaa gacttactgt tactctctca cctttaccag tttgagcttt tttatgtaat
    17041 gctgctaaac ttcctgctgc tagtgctaga cctgcatagt ggtcaccaaa tcctgctgct
    17101 gtatttgctg gagatgttcc tttttccata acagattggc taactcctcc tcttgcgaag
    17161 tatgcagtat agtcaaatcc tggtttatct tttaaaggtc ctttttcacc atatcctaat
    17221 atttgagaga atattaatcc tggatactta tcttttattt ggtcataagc tatacccatt
    17281 ttttctaatg cttgaactct aacattagtt acaaatatgt ctgcttctga taataattta
    17341 tgtaatattt ctactccttc ttttgatttt acattaatac ttacaccctt tttatttcca
    17401 ttttctaatt caaacatagg gttttcatca tctgatgctg gagatttaaa tgttccaccc
    17461 ataactctta ttccatcacc ttctataggt tcaatcttaa taacctctgc accccagtca
    17521 cctaacattt ttgcacaaca tggtgctgcg atgaaacttg aaagttctac tactttaact
    17581 ccttctaaaa gcataacgtt ttcctcctta atagtatcaa ttataaaatt tcgattaaat
    17641 cactcataaa atactttttt ctgatattaa ctgtctgatg tcaaagttcc taatcttatc
    17701 taaaataact taaaaattat taaaattatt ttttattgtt ttattgtctt acacctatat
    17761 tgttaagcat ttatcgtgcc aactttatat ttaatcaaaa tatcgcttga tttgaatgtt
    17821 ttttaacaat atgcaattga ataatctttt tcatatcgtg gcatcttgcc actatccatt
    17881 gcattttgct attctttcta tgcttataaa agtttttttt atatattttt ttatgaaaaa
    17941 gcttgacatt tcaaaaaaag ttttcattgc taaactcaaa aacacatgtt atcattgtaa
    18001 ttaatcagca agttagttaa ctaatttcaa acttagataa ttttttaata atatttaatc
    18061 taaaaaataa caatttcttt gataaatttt agagtaatta ttacattaaa taaaaaatta
    18121 ctaaatatca aaataacaca gggaaaacaa tttcaaaact aaatttttaa ccttaaaaat
    18181 tttgtataaa aaatataacc ttatatgcta catattattt ctaaatgaaa aattaccata
    18241 ataatttaat aattacagga gggtattgat atgaaaatac tagtatttgg agcacgcgat
    18301 tatgaagaac cagtaataaa aaaatggtct gaagaacata aggatgttca agtggatatt
    18361 tatcctgaaa acatgactga agaaaatgta gttaaagcta aagggtatga tggtatatct
    18421 atacaacaaa ctaactatat agataatcct tatatttatg aaactttaaa agatgctggg
    18481 gttaaagtta tagcttcaag aactgcaggg gttgacatga tacattttga tttagttaat
    18541 gaaaatggac ttatcgttac aaacgttcct tcttattcac ctaatgcaat agctgaatta
    18601 gctgttactc aagctatgaa ccttttaaga aagactcctc tagtaaagaa aaaagtctgt
    18661 gaaggtgatt accgttggat agctgaactt cttggaacag aagttagatc tattacagtt
    18721 ggtgttatag gtacaggaaa aataggtgct acttctgcaa aattattcaa aggcctagga
    18781 gctaatgtaa ttgcatttga ccaatatcca aatagtgatt taaatgatat attaacttac
    18841 aaagattctt tagaagacct tctaaaagaa gctgacctta taacattaca tactccttta
    18901 cttgaaggaa caaaacatat gataaataaa gatactctag ctataatgaa ggatggagct
    18961 tacatagtaa atactggccg tggtggttta attaatacag gggatttaat agaagcacta
    19021 gagtcaggaa aaattagagc tgctgccctt gatacatttg aaactgaagg attgttctta
    19081 aacaaaaaaa tgaatcctgg agaattaact gacccagaaa taaataaact tctttctatg
    19141 gaacaagtta tattcactca tcaccttggt ttcttcacta gtacagcgat tgaaaatata
    19201 gtttattcta gtttaagtag tgctgtagaa gttataaaaa caggaactgc tactaataga
    19261 gtaaattagt attttataaa tatatttttt cattaattag ctattatcta acaaaatagc
    19321 ctttaatttc ccaaatataa gagagtcagc tcagtatata ttgagtagac tctcttatat
    19381 tattttttat attaaacttg tactcttctt attcatacca aattaaattt atattaactt
    19441 actctaacta ctcctactct atacatttta cctattttta cttttctaat tatatacatt
    19501 tgaatgggta caatctgtat tttacaaaaa aaacacctaa caaactatga aaattgtatg
    19561 attaatatta atatatttat aagaaaacat aacaattatg ttagctctgg atttttaggg
    19621 agggagtata ttggaaaaaa tgaataaagt tacttctaaa aaaactacct atattgttac
    19681 ttcagcatta tttgcatcca taatatgcct gacaatagca tatatattac atattccagt
    19741 tggtggaaac aatggatatg ttcatatagg agatgctttt atttatctag ctgcaactat
    19801 tttgccaaca aactatgcta tagctgcttc tgcaattggt gctggtctag ctgatttatc
    19861 tacaggtgct gctatctggg taataccaac tataatcata aaaccaatac ttgtattatt
    19921 tttcacttct aaaagtgaca agataataaa taagagaaat atagttgctt cggttgtagc
    19981 aggtatagta ggattagttt tatacatgtt tgctgaaggt ataattattg gaagttttac
    20041 aagtgcattt gtaatgagct tacttggctt attacagcca ataggaagtt ttattgtttt
    20101 tataatctta ggtatggcac ttgataaact tgattttaaa aaacgttatt ttaattaaga
    20161 atttaaattt atggagggaa aattatgaat atcttatata caattgaaaa tagtatctat
    20221 gtaaatataa ctaatacatg tccatgtagt tgtgtttttt gtataagaaa cgaaaaagat
    20281 gaagttgcaa atagtggtag tttatggttg gagcatgagc caagtgtaga tgaagtaaaa
    20341 gaagccttca ataaatacaa tttagacgac tatgatgaaa tcgtattttg tggatatggc
    20401 gaaccattaa tgagaataaa tgaattgata gaggtagcaa agtttataaa agaaaaaagt
    20461 agcataaaga taagaataaa tacaaatggt ctaagtgatt taatacataa caaaaaaaca
    20521 gctattctgt taaaaaatgt tatagatgca gtatcaataa gtcttaatgc cccaaataaa
    20581 gaagcttata atagagttac tcaaccaaag tttggagaaa aatcatttga ttatatgtta
    20641 gattttgcaa gagattgcaa aaaatatata aaagaagtag ctttttcagt agtagatgaa
    20701 atatctccag aagaaataga agaatctaaa caactggcaa aaaaacttga tataccatta
    20761 agggttagac ataagaatta gcaagtaaaa ttataaaaca ttaagaatca cctgaatggt
    20821 ctttcaggtg attcttcctc ttgtattaac attttacaaa atagatttca taccacacta
    20881 ggaatgtgta aatcatccaa atctttctcc aattatcaga gtttccacta atgtagtcat
    20941 taaaaatctc attaatctca tctatgttaa agaatttatc tgctatctca ctattgaatt
    21001 tatctcggat atccttatta tattggtcat cagccatcca tatacgaatt ggcacaataa
    21061 atcctaattt atttctaaaa gcaatctctt ctggtaatgt ttttgatgca gctgtacgaa
    21121 aggcaatttt attctctttt cctttcactt tatattctga tggtattctg gatgcaatat
    21181 caaatattct tgtatctgta agaggcattc tgacttccaa tcctactgca gtactcattt
    21241 tatctatatt aaaataaata tctccttcca accatatttg aatatctaca tttgacatgt
    21301 tagtaagtgt atctagttct tctgtttctt cataaattcc ttttaccact tcaataggaa
    21361 gcacatcagg attgtatttc ttaagaattt ttcttttctc accctcattc ataatgtttg
    21421 tatttccaat ataaaatgtc ttcaaattct ctccataaca ctctgcctta tggtatatat
    21481 tatatccacc aaaaaactca tctgctcctt ctccagagta acagatattt gtgttttcag
    21541 ctgctgcctt gcatccaata gcaaaaacga tagcagatgc atctccagtt ggttgctcca
    21601 tatggtacat tacataaggt actgacgcaa aatactcttc aggtgtaatt atatatctcg
    21661 aatttttaca cccaagaata ttagctgtct gttgtgctaa ttttgattca tcaaaacgct
    21721 cttcctcata accacatgaa cctgtttttt ctatatctga cattgccaat atataagatg
    21781 aatcaacacc tcctgatagg aataactctg cagtttcatt ctctgatttt acctctggca
    21841 taattttctg aagtgttgta tgaatttcat ccgtccattc ctcaatagac ttgctgtggt
    21901 caggatgaaa ttcaggtttc caataacgac caatacatac tgttttattc tgatagacaa
    21961 ggtaatgtcc tggcataagc ttctttactc cacggaagaa tgtattttca cctgctacat
    22021 atgtgaaaga catataaatc tgtaacatgt cttcatttaa ctctttcaca aatcctggtt
    22081 gctccataat cttacgaata gatgaaccat agagaagctt cccattttca gtctcataat
    22141 aatagaaagg ttttacccca aactggtctc ttaggcagaa gagtttcttt tccacagtat
    22201 cccataatgc aaaagcaaac attccatgca tgtgatttgc catatcatat ccccatgtct
    22261 tatatgcttc cactaaaatt gctcgttcac gctcatctct acataatgaa gaatcgattc
    22321 caagttcttc acaaagaact ttccagtttc gaatatgccc attgtattct agaattttta
    22381 tcatatatgt gtcttttcgt gttaaatact tttcttctaa atttatcata tgtgtatcct
    22441 ttcgtattaa aacatttaac ttctaaattt attaaatgtt tatatatatt tattcactta
    22501 taatcactga aaaatcgact tttttcaaag taaattcttt ttgaattagt actaatctgc
    22561 tctttattaa tggcataaat atgattatca agtattatca gtgttataac attgaatttt
    22621 ttattcttca
//
LOCUS       DAEMGG010000056        22495 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEMGG010000056 DAEMGG010000000
VERSION     DAEMGG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.056"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            80..1516
                     /locus_tag="KPK24_002039"
     CDS             80..1516
                     /locus_tag="KPK24_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG2299711.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVNRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            complement(1608..3482)
                     /gene="asnB"
                     /locus_tag="KPK24_002040"
     CDS             complement(1608..3482)
                     /gene="asnB"
                     /locus_tag="KPK24_002040"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG2299712.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            3685..4359
                     /locus_tag="KPK24_002041"
     CDS             3685..4359
                     /locus_tag="KPK24_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG2299713.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            4472..5008
                     /locus_tag="KPK24_002042"
     CDS             4472..5008
                     /locus_tag="KPK24_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG2299714.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(5174..5794)
                     /locus_tag="KPK24_002043"
     CDS             complement(5174..5794)
                     /locus_tag="KPK24_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG2299715.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(6108..8246)
                     /gene="vanT"
                     /locus_tag="KPK24_002044"
     CDS             complement(6108..8246)
                     /gene="vanT"
                     /locus_tag="KPK24_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG2299716.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(8264..9070)
                     /locus_tag="KPK24_002045"
     CDS             complement(8264..9070)
                     /locus_tag="KPK24_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2299717.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(9067..10167)
                     /gene="vanG"
                     /locus_tag="KPK24_002046"
     CDS             complement(9067..10167)
                     /gene="vanG"
                     /locus_tag="KPK24_002046"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG2299718.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(10428..11510)
                     /locus_tag="KPK24_002047"
     CDS             complement(10428..11510)
                     /locus_tag="KPK24_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2299719.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            complement(11560..12261)
                     /locus_tag="KPK24_002048"
     CDS             complement(11560..12261)
                     /locus_tag="KPK24_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG2299720.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(12695..15226)
                     /locus_tag="KPK24_002049"
     CDS             complement(12695..15226)
                     /locus_tag="KPK24_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2299721.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(15334..15951)
                     /locus_tag="KPK24_002050"
     CDS             complement(15334..15951)
                     /locus_tag="KPK24_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG2299722.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(16313..17197)
                     /locus_tag="KPK24_002051"
     CDS             complement(16313..17197)
                     /locus_tag="KPK24_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG2299723.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(17388..18638)
                     /locus_tag="KPK24_002052"
     CDS             complement(17388..18638)
                     /locus_tag="KPK24_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2299724.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(18860..19624)
                     /locus_tag="KPK24_002053"
     CDS             complement(18860..19624)
                     /locus_tag="KPK24_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2299725.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(19618..20343)
                     /locus_tag="KPK24_002054"
     CDS             complement(19618..20343)
                     /locus_tag="KPK24_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299726.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(20345..20713)
                     /locus_tag="KPK24_002055"
     CDS             complement(20345..20713)
                     /locus_tag="KPK24_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2299727.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(20889..22433)
                     /locus_tag="KPK24_002056"
     CDS             complement(20889..22433)
                     /locus_tag="KPK24_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2299728.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         7755 a         3801 c         2352 g         8587 t
ORIGIN      
        1 gaaactacaa aattattgta ctatattgtt ttaagttgtt ttataataga ttatatcatc
       61 ttattatagg agaaaaaata tgatttgtac tattaaacca ttaaagattt caataaaaaa
      121 gtctactgaa cctctaattg aatttatatc tgatactacc tataaaaata agagttcttc
      181 atatattaaa gaagtctact ttacaagata tataggtgtt gatgattcaa ttgataaata
      241 catagaaaaa attaaaaata tagatactta tttttttaat aatcctacaa ttccctatat
      301 tagattaaac aatctaaaaa taaattttga caaagaacaa acagataaga tgcttaaaat
      361 ttttaatatt tatgatagtt ctaagtttaa cagttgggat ttatataata tcgactttcc
      421 atgtaaaata gaaaatgaca ctttaaattg gactaaaaag attgcattta aaaatacttt
      481 agatttattt tccacaagta tgccaaattg tacatcctct atcatcaaaa actttgcagt
      541 taaacttttg tgttggatag actattttct acctaaatta ttttataaca aggtgaagac
      601 tactatttca ccaaagatag tatactttgg aaatattaaa aggcaagaac tttttttctt
      661 atatttttta tcacaacttg ggtgtgatat tttatatata aatccaaata aagatattct
      721 tgatttatat cctgaatgtt caaaattttc tactctattt gaactttcta ggaagactcc
      781 aaatatatta gaaatacctt ttgaaaggca tattacaaat ataaattaca gtgaaaatac
      841 caataagact tctcctacta cgaataaacc taactctaaa aatacactta ataacacaaa
      901 tctaacatct aaaaaaattg tacaagatga ggaagtagaa ctttcttatg aagaccttgc
      961 aaagttatca gcttctgttg ttatgattgt tgtgtgcaat aacgaaaata aaccgtttaa
     1021 gagcggttct ggagtagtaa taaataatga aggttatata cttacgaatc ttcatgtagt
     1081 caatgatggg tattcatttt tagttagatt tgaaaatgat gataaagttt atacatctta
     1141 ccaaattata aaataccatt cagattatga tttagctgtg ataaaggtta atagaaaatg
     1201 caagccaata cctgttaaag tcagtaaaaa acctgtgaga ggtcaaaaaa tagttgcaat
     1261 tggaagccct ttaggtcttt ttaatacagt ttcagatgga attatctcag cttttcgtga
     1321 ttttgaaacg gttcaaatga tacaatttac agctccaatc tctagtggta gttcaggagg
     1381 agctcttttg gatatgtttg gaaacttgct aggattgatt tccgctggat atgatgatgg
     1441 acaaaactta aatttagctg ttgaaagtag tcttgtcaaa atatttgcca ataattttat
     1501 tgagatagta aactaatcaa tcaaaattct acttatccta ttccataata atcaggaggg
     1561 tgaaaacatt gcatgctttt gccctctgat taatattgaa aacagtatta atactcgaca
     1621 tttttatgct caataaataa ttgatgccat agacaaaatg taaatatagt ccatattttt
     1681 ctcgaattat ctctatgacc tttaagatgc gtatctaaaa gttttattgc atatttttta
     1741 tctataaatt catctacatt tgaattataa atagtttcct ttacaatatc ccctagagaa
     1801 tcttttaacc aaactcttat tggagttgga aatcctaatt ttttcctttg aacaacatgt
     1861 tctgggacta tttctctaaa cgcctctcta agtaaaactt ttgtattatt cttatttatt
     1921 ttttgctcca ttcttaaatt ttttgctaca tcaaatacat ctttatataa aaatggtact
     1981 ctaagttcaa cctgctcagc tgtagaaagt ttgctaattt tttgaagtat atcgccttga
     2041 agccatgtat tcacatctat atgttgcatt ttacttatat aatcgtaatt atttttttgt
     2101 gcatctctat acaaattaga caacaggtat tcatatatat tcttttcctt atacttaaaa
     2161 aagaatcttt ttacttcgct gttttcaaaa acttttgcat ttcctatata tcttttttct
     2221 aatggtgttg tagctctata aagataattt cttcctttta tgtttggcat aagttctgaa
     2281 actcttccta atatactttt aatataagta ggtgaattga caactgattt catagtacta
     2341 tactctttat atatattata tcctccaaat aattcatctg ctccttctcc agacaatgct
     2401 actttaacat gtttacctgc ctctttaatt aagaaataaa gacctacttc tgaagggtct
     2461 gctatagggt catccaaaga ataaattaca ttcggtaatg attttatata ttcatcttgt
     2521 gttatattaa tctggatatt ttctatacct aacttatcag ctgttttctt tgctacttct
     2581 aattcactat atcctttgac actaaagcct gcagaaaaag attttatatt tggattaatt
     2641 tgtgaagcca cagtagttat aatagatgaa tctattccac cagacaaaaa tgttccaatt
     2701 tctttctcct cttccatatg agttactatt gaatctacta ctatatttct tatatcatcc
     2761 tcgttcacat ctctattgcc taagtgattt ttagtataaa aaaattttgt actacttcta
     2821 aactcaagtt tattatatct ctttaaagat agtataccat tttctaccct aaaatagtga
     2881 cctgctggta tcagccttat tccttgtatc atagtatctt caggaagtac atattgaaat
     2941 gaaaaataac tttgtaagct tctttcattt atatttaaat ttttcatata ttcaagtaaa
     3001 aacttatact ctgaggaaaa aattattgca ttttccttat ttatataata aagtggtttt
     3061 atcccaaata aatctcttgc tccaaatata ctttcatttt ctctatcata gattataaag
     3121 ttgaacattc ctttaatctt atgtacacaa ttcttaccaa attctatata agatgccaat
     3181 aagacttcaa tatcatcatt tgtagtaaaa ttgtgtcctt ttttaattaa ttcttctttt
     3241 atttcatgat agttatatat atttccatta aaaacaataa cttttccatc tttttcaaat
     3301 ggtttatttc cataaatagc atcaattaca ttaaatcttt caaaatcaaa agttttttcc
     3361 tcatccttat actctattct actagaaagt cttgtattat ttacagattc ttttatttta
     3421 aatttaatat cttctttttt agatatatca cctttataat aaattgaaat attgccgtcc
     3481 atataatcaa aattcctttc tgtcctttag tcttattcta tatttcatag ttttgtcttt
     3541 ggctttaaaa cttattcaat ttaaaatgaa attgctatta aattcttata aattatctgt
     3601 taatatttca tatttttttt atttctagaa atactatatt aggaggtgat ttttatgaag
     3661 aaaaaaatat taataccagt tattatgtct ttatttataa tctcacagtg cataacttca
     3721 tttgctttta cacctgaaaa taacaaattt aaggttaaac cattacctta tgcatatgat
     3781 gcacttgaac cttatataga taaagaaaca atgaaactgc atcatgataa gcattatcaa
     3841 gcttatgttg ataaattaaa tgctgctctt gaaaaatatc ctgagcttta taattattct
     3901 ttatgtgaat tattgcaaaa tttagattct ttacctaaag atattgctac aactgtaaga
     3961 aataatgcag gtggagctta taatcataaa ttcttttttg atataatgac gccagaaaaa
     4021 accatacctt ctgaatcttt aaaagaagct attgatagag actttggttc ttttgaaaaa
     4081 tttaagcaag agttccaaaa atctgcttta gatgtctttg gttctggttg ggcttggctt
     4141 gtagctacta aagatggcaa attatctatt atgactactc caaatcagga tagccctgta
     4201 agtaaaaacc taactcctat aataggactt gatgtttggg agcatgctta ctatttaaaa
     4261 tatcaaaata gaagaaatga atacattgac aactggttta atgtagtaaa ttggaatggt
     4321 gctttagaaa attacaaaaa tttaaaatct caagattaat taaatattaa aatccttttg
     4381 aaaaaaactt atgtttgcta tataatataa taaaagatag tctaaattta ccaatataat
     4441 gtttatagtg acgattcagg aggattttta tatgataaaa gaaacacatg aaaggggagg
     4501 ttatatattc gcacttttga tactcccctt tataaacaat ctatacctag ttaaatacga
     4561 tattgcatat aaaatagtat taattataat ttatatttat tttgcatatt taggttcttt
     4621 atttccagat atagatatga gaggttctta cataagtaaa aaatttatgt taatatacaa
     4681 attatttgga agtaggttta gacatagagg gtttactcac agtctaattg ccttattact
     4741 tatttatagt ttttttaaat ctctgacaat cttcactaac aataatatag ttttctcatg
     4801 tttgtccagt gggtttataa taggatatct atctcacttg tgtcttgacc ttattactaa
     4861 agaaggtatt gaattgtttt atcccattac cataaacatt tctttactac ctataaaaac
     4921 taactcaaaa acagaaaaat tcatatctaa gctattaaat ttcatagtta tttttttgat
     4981 tggatatcaa ttttatattt tattatagtt gtagaaaaaa taaataacaa tattttgaac
     5041 taagtataaa taagttttat atattttagt ttacaaaaga actaaaaatt ttgtacctta
     5101 atacaaaaaa taaaacgtat cttaaaataa tttagtacta aatcatttta agatacgttt
     5161 tatttcgtac aagctaaatc attttatcat ctttatcatt gtgatgtttt ttatgatgga
     5221 aatcataatt atgattttca agatttttat ttactctatt tccaatcttc catgacaaaa
     5281 acaccaataa tataaatgat gctatcttta aaggacttgt tataaaacct tctatatcac
     5341 ttgccacgta acttataact ataaacatta caaattttcc tgccaacatt gctggcacaa
     5401 aatcttttaa atctttttta ccaaaagctg atgcgatagt aactaaacaa ccgggcataa
     5461 atggacaggc atacgctata aataataact taaatccctg tttatccatc caacttattg
     5521 ctttttcagt tttactattt ctcaacttat taaaaagctt actatcatta aatctgctta
     5581 ttaataagaa taatgaagtt gtcccaagtc ctgagccaat ccatgataac aaacatccaa
     5641 taaataatcc ttgtattgca gcatttgctg taactatagc aacaagtggt aaggctggta
     5701 taaagctttc tataaatgca cttaataagc cgattattgt ggctaacacc caataatttt
     5761 gagtcaaatc aagtagttct tgtatatact gcatattgct tttccccttt tttaacttta
     5821 actattatta tatactctat atgtttatta tataccaatc tcaaaaaatg aatctaatat
     5881 taaaaagtaa ataatctata taagtttaca gttaaggtat aattatttta ttatatatta
     5941 taacaaaaaa ggtgatgttt caaaataaaa atctgaaaca tcaccttttt ataacttaaa
     6001 ttgtcatgta agttataaaa aatatatatt agacataata ttactaaaaa tgtctagtca
     6061 gagtaaaata aattacacag tacaacaaat attactattt aattgagtta cttctttata
     6121 acatttaacc ttcttcctac tctgctcaat aattcattcg atatactaca tgagttattt
     6181 gcaacttcag aagcagataa ttcagataaa ttatctcttc ctattattat tgccgtatta
     6241 ccaacttcta cattaggtat atctgtaata tcaacagcaa gttggtccat gcaaattcga
     6301 cccactattg ctgctctaca accatttatt attacgtaac tttttccaca ggataaattt
     6361 ctaggtatac catcggcata gccaatagat aatatagcaa ttacgctatc tctgcttgca
     6421 ataaagactc ttccatatcc aaaactatca tctttttgta tttttctaat aagaattact
     6481 tgtgatttta aggataaaac aggtcgtaaa tcaagttgca atttagtcgt atcatttgga
     6541 gaacttaata tcccgtataa tgcaattcca attcttacat aattacactt aagattggga
     6601 taattaaaaa aaccataact actttgaata tgtactttag gaatttttat accttttttt
     6661 aacagaaaat caattaactt ataaaaacat tcaatttgtt tatttgtaaa gtctacatct
     6721 tcatctttca gactatctga aacacacaag tgtgtaaata tgccacatat atctaagtat
     6781 tttaatttaa atgcctttaa aattgacctc acatcattaa catcaaaacc cagtctgtgc
     6841 atacctgtat caattttaat gtgtgatttt atgttgtatt tctgtttgtt taaagagata
     6901 gcatgattaa ggtcaataac actctgtgta agattgtatt tatgaagttc ctttgccctt
     6961 attgggttag tatatccaag aatcaatatt tctcctagta ttccatatcg acgtaaatca
     7021 atcccctcat ctattgttgc tactgcaaat gcttttacac ctatttggtt tgcacaagtc
     7081 gctacttcaa aagcactatg accataggca ttagctttta caactgccat caattcacaa
     7141 tcttctggca tagctttttg tatcatcttt acattatgtt ccaaattatt taaatcaatt
     7201 tcaatccaag ctctattggt atttacctta tgttctcctt tcaagtcttt ttttagaggt
     7261 ttaagaatca atataataat tatagaagct ataaaagatg ctatacttac cactatataa
     7321 tgaataaggc tattgtcaac caagacagtt tgtattccga atattttaga aaacatacga
     7381 ataacaacaa tcatcatagg atgtataata taaataatta atgatgatgt tcgcaataac
     7441 ctagctcgag aacctttcaa aaatgttaag gctgtaaata agaaatacat acatggcaat
     7501 aacataatat acatgctatc atgacgctgt acccctagtt tatgtaaaaa cattccctca
     7561 cataacataa gtaagaatga tataagaaat ccaatcaagc tatttttagt tgacatataa
     7621 ttagatttat tagatattat tccaccaagt atgaaaaata caggtgcaaa aaatatacca
     7681 tttcgtgtat aatctgatac ttcaaaaata tacccatata aattctttaa tagtggtatt
     7741 ttttctaaaa aaccataata actatcacca aacataccaa ttacataaat aacaatagtg
     7801 attactagtg agtactttaa acctagtctt tttacaagaa agtatgcaat acttgcacca
     7861 ataattgatg caggtagata ccataaatga tataaagtcc catcaaatac aatgtcctta
     7921 ataatattgg gcactaaatt attcatggaa aaatatccag tgtaaatatt taagggaata
     7981 taaattacaa tagctatccc gtaaataaat aatacatttt ttagaaaaga tttcagttta
     8041 ttacaattat aattatatgt tgaaattaga aaaaagcctg atagcataaa aaagaatggc
     8101 acagcaatac gagcaattat actagttaac acaaagtcag ccattttact atatgataat
     8161 aatggagatg tatgaattgc aattataagt aatgcagcta taatcctaaa ataatcaagc
     8221 cctgcataag atttattcct aatcattctt gttcctccat ttcttagtcc tttctatttc
     8281 tccaaaatgt cacaataaaa ccatccttat tgttacccga tatttgatat acacattgtt
     8341 caggtataga gattctcact tcttctttag tatgaatagg cacataaaag atttctataa
     8401 ttttttcttt atcttctatt tttaaacaat ttgtatacga aaatctctta atatattcta
     8461 tatattcttc aagagcgaga ttcattttat tgataatctc agaatgcgga tatcccacat
     8521 aacgaaaatg ccatggttct tgagatattc ctgttatttc ttctttttta cactgataac
     8581 gctctataaa accataatgt agtgctgcct tacgaaactc attacaaata ccattataag
     8641 gaaattcagg gcagataaaa tctataactt ctttctttaa ccctaaatca atagcaagcc
     8701 ctgtctgatg ttcactgtga tttggaagtg ctacaaactt tttagtaaag tcttttccat
     8761 tgtcacgcag agaacttgaa tatatatctt gttgttcctc tgcagtacga tatccactta
     8821 caggtactat ttcatttatt ccacatatac tttttaaaat attttgtaaa atgtttgtag
     8881 cttcacgttt cataagaata cttggaaacc ttgtatctac aggaattaaa tctatatctt
     8941 cagatgtttt aactggtaaa aaagcattta ctagaattag attcccatca taaactttat
     9001 ctttagtcaa taagatagta tttgtcattt tatcttcttt atttttatct aataaaatat
     9061 tattcatcat tttatgtaca cctcaattat cttgtccaat atttcttcaa aagacaatcc
     9121 aatacctttc atcatatttg gataacgact atgagaagta aagccaggaa ttgtattgac
     9181 ttcattaaaa ataatttcac cagatggagt taaaaacata tctactctag caaatccaga
     9241 acatccaaga gttctataaa taatttttgc tgtctcttgt attcttttct cacaatccaa
     9301 atcaattcta gctggcatat gaatttttga acttttcaat gtatattttt ctgtataatc
     9361 aaaaaatcct tttgacaatt ctatttcatc tactcttcca acaagtaagt catcgtttcc
     9421 taaaactgca caccctacct caaaaccatc aatattttcc tcaattatta cttcattatc
     9481 atgttcaaaa gccaagtcta ctgctacttg taactcttct tttttataaa ttttactaat
     9541 accaaatgat gaacctgcac gcacaggttt tataaataat ggataagtta agtcttttga
     9601 cagttcagcc acattaaatt tatctgagta ttttagtaat attgattttg gtactttaat
     9661 accttctgaa tgaacaagtt tatgtgattt gtctttgtcc atacataatg ctgaagaaag
     9721 agtaccacat cctataacct gtatacctgc caattcaaat aacccctgca ctgttccatc
     9781 ttctccattt tttccatgca gtacaggaaa cacaaaatca agtttagtgt atttaatatc
     9841 attttcacat gattcaatag cactatttgg aatatgattc tgagaaacag catcaggaat
     9901 taaaaattca ataataccat ttttgacacg gttctgcgaa acaataactg gaattatatc
     9961 agtatccctt tcaaaccaag tgttattctg gatattatta taatctccaa tataatgata
    10021 ccaattaccc tcccttgtaa ttccaattgg tataatatca tatttttccg tattcaaatt
    10081 ctcaagtact gcaaaagctg attgtaatga tacatcatat tctgttgagt ttccaccaaa
    10141 tataactgca atttttttat tattcatctt tgattctcct ttgttgattt atttattttt
    10201 atacaatcta cagaaacatg ttaaaatact ttttctattt caaatccaat caaccttcac
    10261 ttacaattgc atctgttcca cataatatat tttcacattt ttctattgat ataattactg
    10321 tatcaaaata aaagcactct tgttttgata cagtaattat atcaataaca agagtgctct
    10381 ttatttattt atcctaaaga atttcctatt tttttatact taaattctta caaatttcct
    10441 acaatcggaa taataacctt aaagataatt atattgttct cgctatgagc tgttattgtc
    10501 ccgttatgaa gctccactat ttctttagct atagcaagtc ccaacccagc tctaccactg
    10561 tttgaattac gagatgtatc caacctgtaa aattgttcaa aaatacgctc aagtttttcc
    10621 tctggaatcg tattgctatg atttataaat ttaagcttta tataatcatc ctcctgtaca
    10681 gctgttattt caatagtagt atcttcaaaa ctataataaa cagcatttcg taataagtta
    10741 tcaaatacac gttgcatttt attaacatca caccttatca tcatatttgg tgtaacattc
    10801 agtgtatatt ttaaattctt actcatcaaa gtaggtttaa actcatatgt tagttgttca
    10861 agcatacgag ttaagcttac tgtactgtat tcaagtgata tatttgataa attaaacctt
    10921 gtaatttcaa agaattcatt aattaaatct tcaagacgct ctgccttatc caacgaaata
    10981 gataaatatt tttttcgcag ttcttcagaa atctgacttt catctcgaag tagtgtcaga
    11041 taccctataa ctgatgtaag tggtgttttt aaatcatgtg caagataaac aataagttca
    11101 ttcttacgtt gttctgccat ttgtgtctca aatttacgtt gttcaagtat atgtttaatt
    11161 gagtttattt tcttttctat tgccaataac tctggagata aagccacttc tccaacatct
    11221 tctttgataa gagagtcaat accttgattt atttcactaa aatattttgt aaaccaattt
    11281 aaataaaccc ggaatattat aaaaaacaca atcaccatag ctagtatcat gaagagctcc
    11341 atgctattac ggaaaatctg ctgatataaa ttcaatgcag ctttatattc catacgaaaa
    11401 gcattttcaa agaatgatac tatccagata gaaaacttac catacaatat aaaagaatat
    11461 aaaatccata tgaaaataat ggctataaat gccattaaag ctgttctcat aaaaatttta
    11521 tttctcactt gagtaaagtc aggattgact ttaatatttt tacttttcaa ttgtataacc
    11581 tactccccat acagttttta taaatttcgg gtttttagaa ggttcatgaa ttttttcacg
    11641 taatcgccct atatgagcca taactgtgtt attattatca agatattttt ctccccatac
    11701 agcttcaaac aattcttcag aaggaaccac ttttccttga tgttcacata aataccaaag
    11761 aatagaaaat tcaatcggtg ttaatgctac ttctttccca aataaactac atttatgtgt
    11821 ttctttattg ataatcaatc ctttaatatc atactcatta acaattatcg actgttgttc
    11881 ataagaatta ttatatcgca tatatcttct taattgtgtc tttactcttg caacaacttc
    11941 caaagggtta aatggctttg ttatataatc atcagcacct atggttaaac ccattatctt
    12001 gtctatatct tcaattttag ctgttaacat aatgatagga taatagtatt tttctcgaat
    12061 tttctggcaa atatgaaatc catcaatatc aggaagcata atatctaaaa tagccatatc
    12121 caaattttta gattcaatac attctagagc ttccttacct gtataaaact tgtatactgt
    12181 aaaaccttca ttttttaaat atacttcaat taaatctgct atttcttttt catcatccac
    12241 aatcaaaata ttttcgttca tagattattt ttcactctcc ttattatcga ggtcatcatt
    12301 tataatattg gtaaatttac atatcaaata attacaagca tatttgtaac taatttttat
    12361 aagaaaagaa ctactacatc agaatgtgtt gatgctacta ttggaacaca aaaactgaga
    12421 ttaattgtta ttataaaaac tcccctaaca cgtccttatt atcgcataac aggtattaac
    12481 tttcaataaa aatatctatc agtcaaacac aattcaattt attataccag agacaatcca
    12541 tataaaaaac aattaaacct atgtatgtaa ccacagacat tactaaaatt tccatgttat
    12601 tttatataaa aattcaaaaa aggagttgaa tttaaaattt gattagaaaa aaatcaattt
    12661 tgagacaact cctttttatt ttaatgtatt taaattatag tatatcagcc ttcataatat
    12721 cctcaaaatc acctttttca gatattaatc ttatcaattt aacagtacag cttaaatcat
    12781 ttataagtat agctccaact aatgaaccat ctttcataaa taatttttta tatattcttt
    12841 gaccttcatc ttttgatgaa atttctttat caaaattttg tatattacca atactaaata
    12901 cttttacatt cattgcatct aatgaaactg gatatacttc actttcaaat gtcaaattat
    12961 ctccacttgc atttgcccct gctatttttc ccatttctac tgaagatggc catattgcta
    13021 aactaatatt tcccacttgt gcaacatctc cacaagcata tatatcttta acatttgtct
    13081 gtaatgtctt atctacaaca atacctctat caatttttac agatgtatcc tgaaccattt
    13141 gagtgtttgc tcttacacca attgaaaata ctaccatatc acagtcgaca ctatctccat
    13201 ctttaaatac aactttttga gcttttccat caccttcaat accatcaact gctacaccca
    13261 atcttacatc tattcctgta tctcttacac attgttctaa tattttacta ccttctttat
    13321 caagttgttt tgatagtatg ctatccatgg cttcaactac tactacttct aatcctttta
    13381 gtctaaattc ccatgcagct tcaagtccca ataaaccgcc accaatcact acaactttct
    13441 tagatttttc tagtgcttct tttacactat ataaatcttt tatatttcta agagtaaaca
    13501 cattttctaa atcatggccc ttaattggtg gtataaagtt tctacttcct gtagctatta
    13561 caagcttatc aaatttaata gctccatcat tcactataat agttttattc acttcatcta
    13621 atttatctac attcgttcct aatattacta ctatattatt tttatcatac caatctttat
    13681 cttccatgta gaaatctgaa ccaagttctt catttatacc atcagaaagt gcaggtctat
    13741 agtaagtcaa ctcatcttca ttagaaatca tagttatctt acaagtttta tttctttttc
    13801 ttatagcatc tgctgcataa aaaccagctg ctccatttcc tactattacg aaatattcat
    13861 cagtatcttt tctaaaagta acttcatctt cttcaacttc tataaattgg tcttccttag
    13921 ctccacatac agggcataca ctaggagcac tatcaccttc aaaaacttca ttacaaatta
    13981 cacatttaaa tttcttagtt ttctttttac ttccaaaagt agaatttaac ttatctagga
    14041 atttacctgt ataagaatat aatttatcaa tctcttcatt tgatggttta aagttaacag
    14101 caaaaggttt tactgctgtc attctaagct gacttattct ctccatagca ttttctatac
    14161 cttctccact ccatccatat gaaccaaata ctgaagcaac ttttccacca tgaagaactg
    14221 ggtttaacga tattaatata tcccatatag gctttaacgc atctcctaat attgttggtg
    14281 ttccaaataa tacaccttgt gatactgcta tttttgccaa aatatcttct tgcttatgct
    14341 ctattacatc aaataaattt actttatagt tgctattttt ttcaatatat gcttttatag
    14401 cttctgccat aatctttgta tatccatgtg cagaaacata acaaattgta acttcttttt
    14461 ctaactttat ttgttcattt acactccagt tgtaataaag gtctataatt tttctaggat
    14521 tttcagttaa tactggacca tgacctggac atacagtatc tatatctaaa tcttttatct
    14581 tttctatagc agttaccata gatggtttaa atggacccat tatacaatca taatagtatc
    14641 ttaaagcatc caagtaatcc ttttcttcat tttcatcaag tgtatttact atcttatcat
    14701 tactatagtg actaccaaat gaatcacatg ttacaagagt tttatcctct tttatatatg
    14761 tgtatatggt atctggccaa tgaagcatag gtacagagaa aaattctaaa gttttgtccc
    14821 caattgataa tgtatctcca tctgtaactg caacatattc aaagtctttg tttacgattt
    14881 ccttaagata tttaattgct gtttctgaag ctactacttt tgcattttta gctaaatcca
    14941 aaagcttttc aacgcttcct gcatggtctg gttcagtatg acttacaaca atatagtcta
    15001 ttttttcaaa atctatattt aaatcattta atctttcaat ataattgtca aaatgcttat
    15061 ctttaactgt ctcaaataaa actgtttttt ctgttccttt taatacatat gaattataag
    15121 tagttccata aggtgtatac ataattatgt caaatactct taaatcaggg tcaagcgaac
    15181 ctacccaata taaattttct tttattttta gtgatttcaa tgacataccg tactcctcct
    15241 aaaatagtta tttagtctct ttataccctg cttgttatat tttaaacaaa atttattcaa
    15301 aaaagaaatt attttaaaga tagtttaaat cttctaatct tctttttcta ccttcaatac
    15361 agttcccttt ttagaatcta cttttatgtc atattcattt ttatctttat ctattcttat
    15421 tttataaaaa gtaacatcta aatctttgtc taaactccat tctttaacta ttccattttg
    15481 ttcttttaat gcaatttcca tagcttgttg aggtgtcatt atgtcattta aatctaattt
    15541 ttcttttcta gcaactccat ttaaatcttc tgaatccaac ttttctgttt tatcttctaa
    15601 tacatctttt gtattagcat caattttcat cttatattca ttattatcat ctacaccttc
    15661 cacagtataa acaaatttat ttaaatctct ctcaagactt aaatctacta tatcagcatc
    15721 cttatattta tctttaaata tgtttattgc atctgtgtat ggcactttaa aatcttgttc
    15781 cattaaattt tcattatttt tttcatcttt taaagcgtta tttgatgcat ttgcctcctt
    15841 ttttgtatta tcttgtgctt gtcctgaatc cttagttgga ctcccactac atgcagttaa
    15901 taaacccgct ataagcatac ttaccactaa taaatacgtt tttttacaca taaaaaccct
    15961 ccttaaatat attaatttat tctatattat gccctctaaa ccctttattt acccttttat
    16021 attaaaaatt cctcctattt tatcaatttt aaaactttat atttactaaa attaaagtta
    16081 cataaaaatt atactgtctc aaaatggaaa caagcctctt tttcattgtt gaaataataa
    16141 aaatatattt tatttcgtac aaaaagagtg cctcatgaac taaaaagttc attttgagac
    16201 actcccattt ttttatattt ttacttacaa aataacagta cttattaatt ttttacaaac
    16261 cttataagta tatctatatg atattcttaa atttaaacac tatattaaaa aattaaattt
    16321 tgtttaattc ttttaccgtt aaatccattt taggatatgg aatcgtaatt ccttcttcgt
    16381 caaacctaac tttaacctct tctagtaaat caagataaat tgtccaataa tcttctgtct
    16441 tgcaccaaac tctagttgca aatttaatag cattatctcc atgttctact actcctataa
    16501 atggttctgg ttcatttatt attaatttat gattattaac aatatcttta agtactcttc
    16561 taacatgtag tatatcatct tcatacccta cactaaaagt caaatcaact cgtctagtat
    16621 tctttgctga atagtttatt agactatcat ttgatacaga accattaggt ataagtatct
    16681 gtttattatc tggagtaacc aattgtgtat aaaaaagacc tatttgttca accgttcctc
    16741 catgacctgc tgcttctacg taatctccta ctttaaaagg tctaatcaat agtataataa
    16801 atcctccagc aaaatttgat agactacctt gtaaagccaa acctattgct acccctgctg
    16861 atgctatcac agcagcaaaa ctggacaaac tcattcccca atattccaga acaatgatta
    16921 taacggcaac tttaagtaca ctcaaaagaa gagactttaa aaactttctt aaagttaaat
    16981 caatatctct tttactaagt aataacataa catggtttac aaattttttt attattttaa
    17041 atccaataga aagtataagt atgcctatta taagttttac tccgcttgta gtagcccatt
    17101 ctagcagact ttccattata cctcccatat ccatcttatt aatatctaaa ttatttaaat
    17161 ctaacttgct tatatcatct attttcgtcg tatccataaa aaactcctta ccttatattt
    17221 tagaaccaat attatagttt aaagttctta ttttctattt aatataatac aataccattt
    17281 gtacatagta aacaagattt atattccaat tatacatatt aggaaaatgc aaactatttg
    17341 ttatatatat gcaaaatata atactaaatt aaactattaa taaaacacta tatagcctta
    17401 aaatcatcat tttttacccc tatatgagat gatactatag ttataatact tagtactata
    17461 aatactacaa aacctttttt atatccatca actatattat atgaatttac ttgtaaagag
    17521 atagctataa tcaaattaat aattccactt cctataaatt caaaagtatt aataacacta
    17581 gttgccagtc cagaataaat ttctttattt ttatacttgg catcattaaa aacctgtaga
    17641 tgtgacataa ctatacatcc cataataaag aatgatattg gtaaaactat aattggtggt
    17701 tttacctggt atacaactac aattacaatc catataaaca aatctatcat tgctccaaat
    17761 tttattatat taaatttgct agaccttatt ttagcaaata caaaatccat aattattgaa
    17821 ccaaatataa atccatatgt aaaaaatgat actataaacg cagaaaaact cttactcaca
    17881 ccataaacgt ccattatgta tcttactccc cacaagctta taaatgcact tgtaagacca
    17941 acaaaagaga acattatcat tgaattatac catgttgatt tgttctttat aacagactta
    18001 ataccatcaa caatattaac cttctctgac ttttcatagg gttcagtatc tacattgaat
    18061 ccatactctt ttggtgtatc tctaacaata atatacataa aacatccaat tacaacacca
    18121 attataccga ttaataaaaa tgaatttctc caaccaacaa gttcagacaa aaacacaagt
    18181 ggaaaagtag caagaactcc acctaaattt cctacaaatg aaaatttagc tgttgcagaa
    18241 gcaaattctg acttattaaa ccatctccct tgaattttta aaatacaaag taatattact
    18301 gatgttcctg cccctacaat tacacgagaa atatatgcta attctatact ttgaatcaaa
    18361 ccaaataaaa ttgaccctaa acttgccatt attattccaa gactgctaat ttttcttgct
    18421 ccatatttat caatcaaaat tcctgcaggt atttgcataa atgcatatgc atataggcaa
    18481 aaggatgcta tatttgaaat ttgtatagaa ttaaaaccta actcatttgc taaattgtct
    18541 gaaacaacag cagttgacat ccttaaaaat aatactaaaa caaatgaaaa tgtaattata
    18601 ccccaaacac tccacttagc ctttgtataa cttttcatat cttctccctc aaatccatat
    18661 atttttaaat tttcttgata taacttatat aaagtgtata gttattctta caaattttta
    18721 cacgcaatat cattatacaa catatgtata tatttaacta gaaataaaac actattcata
    18781 ttatatacat gaattaacta cataaataat aagagccatc ttttaacatt actcaaaaga
    18841 tagctccata taaaaacatt tattctaaat ttaatttttt atctaatata aattttgtag
    18901 cgaaaaataa cgctattgca actacaagac taccaagtat tgcaagcata aaactaggac
    18961 tctgataaag atgatatacc atatttgtat cttcatttgg taagatgctt ccaactgcat
    19021 ctccaacaat tgatattact atatttatag ctataaaaga agcaaatgcc actatgtttc
    19081 tatgtttatt gaattttggc aattgtccca ttgagattga tgtatataat aacaaaataa
    19141 atgatgaata tgaaataaac attactacta ctaataataa aatatctaaa aagttttcag
    19201 aaatccattt aaaactagtg ttaaacaaat ctaaaatttc aggtagtaaa attccagaag
    19261 taccaaaaag cataagaact ccaaaagtga atactgcaac tataaaacta agtacagcgt
    19321 atataagtgc tgttatacat ttagataata taagtgaact agtgctaaca ggaagagtaa
    19381 acattaaata accttcatcc cccaaaagat tttttctaaa cctttgaatt gtaactacaa
    19441 tagttaaaac tcctaatgcc ataaaaagac ttgttaatac tagtattcct attccctgta
    19501 cctgaagtat atttgtattc ataaatattc cattaaatac agctactatc aatatggcta
    19561 tatataacgg tataaatatt cttccacttg cttttaactc atattttaat aatttaccta
    19621 acatttaaat tcctccctaa ataatgcatc tatagatttt cctttttctt ctcttattgc
    19681 ttcaacgtct ccttgtaaca ctatttctcc ttttgatatg aatattatct catcacatat
    19741 gttttctatt tcacttatta agtgggtagc aatcagtaaa gtactatctt cagaataatt
    19801 ttttaatata gtcttaagta tatatgacct tgcagctggg tcaacaccac ctataggttc
    19861 atccaatata tatatgctcg catttcttga cattactaaa ataagttgaa ctttttcttt
    19921 agttccttta gacattgtct ttagcttttc atttacatca atatctaagc ttttaatcat
    19981 ctcatttgct cttctaacat caaaatcact ataaaaatca tagaaaaact taagtaaatc
    20041 tgataccttc atccaatcat ttaaataagt tctttctggt aaatatgaaa ctattttttt
    20101 agtatctatg gatggtttca ttccatttat cataatttca ccattatcag gctgtaataa
    20161 tccattcatt agcttaatca ttgtactttt gccacttcca ttaggtccta acagcccgac
    20221 tatttttccc tttggtatat ttaaatcaat attttttaaa acattcttag ttccataact
    20281 cttgtttaca cctttaaatt caactattcc atcttgagaa acattgctta tatttttatc
    20341 cattttataa ttctcctttc agattttctg taataagttt tagcatttca tctctatcat
    20401 atccaagctt ttctaaggtt acttttaatg tatttatctc cacattagct atttcctctt
    20461 tcatagcttt tattttttcc ttatcatctg ttacaaatct tcctttagtt ctctgactat
    20521 aaactaaacc ttgtctttca agttctgtta gagccttttg cattgtattt ggattaactt
    20581 ccgcatcttc tgctagtgct ctcactgttt ctaatttaga tcctatttta atttcccctg
    20641 aaattatttt taactttaaa tgttctacta attgtatata tataggtttg ttattatcaa
    20701 gttcccattc catgttcatg cctccttatt atattatcgt attaagttgt taatacaata
    20761 atataataat acaaagtata tgtcaattac tttttagtaa aagtataaaa aattttacaa
    20821 aaaaaatgaa cactacatta gagtccattt ttaacacaat ttattaaatt ctttatatta
    20881 cagattaatt accctcttac caagttagat acaattaaat gttcaaattc ttcaattggc
    20941 attggctttc caaacaaata tccttgtgcc atatcacact ttacttcttt taaaagttta
    21001 aactgttctt ctgtctcaac tccttctgat atgactttca tacccaactc ttttgccatt
    21061 tctattactt tagtaacaat tattttgctt cttttactat tataagtttc cataataaat
    21121 ttttggtcaa gttttaagat atccatagga atttctttta acatatttaa tgatgagtac
    21181 cctgaaccaa aatcatccat agatattaaa aatcctattt tttttaaatt attcattata
    21241 tcaattaaaa tatcaaaatt atcaaatatg atactctccg ttaattcaag ttctatatac
    21301 tttgcaggta tattgtattt atcaattata tttttgtatg tttctataaa attattgcaa
    21361 tataaatgta tccttgaaac attaactgaa acagtaactg gatttattcc tgcatcaatc
    21421 cattttctaa ggtatataca tacttcttca agcacatata aatctaaatt tataacaaaa
    21481 ccgtttcttt caaaaaatgg gataaattca tttggaggta ttagcccttt atcaggacgt
    21541 ttccacctaa caagtgcttc tgaaccttgt atctctcctg tattaagttc tatcttaggt
    21601 tgtaagtata taataaattc tttattttct agacttgaaa acattgtatt ttccatctct
    21661 ttttctttta tctcttggtc atgaatctta ttatcataaa atgcatatga acttttatgt
    21721 cctccctttg ccattttatg aggtatatta gccctatcta tgattgagtt gatatctcta
    21781 tcttctggaa gtataaaata gattccacaa cttaaaacta acttatattt ttcttcttga
    21841 ttattattaa acaagtctaa ttcttcaaat atagttttta acctctttgt tatatcttct
    21901 tcttctatat atttcataat tataataaaa ttatccgcag atattcttgc aaacatttcc
    21961 tcttcaaata tattgctctt aataatcttt gttaaattac gcaaaatttc attcccaact
    22021 tcatatccaa ataaatcatt tatatattta aatttatcaa tatctatata taataaagca
    22081 tactttactt gagtattttt acatagtatt ttgttagcat caattacaaa tttatctata
    22141 gtatttgctc ctgtaagatt atctgtatat gctatccttt gcaatttaat tttaaaattt
    22201 aatttatcat atattatata gaatataagt attacacata ttatagccat aataagtatt
    22261 atttgtattg catgtttgct aataagctga ttattagaca caatagactc aaagttattt
    22321 atggagttgt ttttatagag tttgcataaa gaatagactg gtgttgtgta tgtaatttgt
    22381 aaaattatac aaataaataa aaaaaacatc aatttttttt gtaaattcct cataaaaatt
    22441 tcctcccatt aaaacacctc tattttatta gcaaataagt acatctcaaa agttt
//
LOCUS       DAEMGG010000057        22436 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEMGG010000057 DAEMGG010000000
VERSION     DAEMGG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.057"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..456
                     /gene="tuf"
                     /locus_tag="KPK24_002057"
     CDS             <1..456
                     /gene="tuf"
                     /locus_tag="KPK24_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG2299729.1"
                     /translation="LVGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEI
                     ERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLP
                     EGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGVVATIIE"
     gene            828..1139
                     /gene="rpsJ"
                     /locus_tag="KPK24_002058"
     CDS             828..1139
                     /gene="rpsJ"
                     /locus_tag="KPK24_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG2299730.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            1231..1860
                     /gene="rplC"
                     /locus_tag="KPK24_002059"
     CDS             1231..1860
                     /gene="rplC"
                     /locus_tag="KPK24_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG2299731.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1890..2513
                     /gene="rplD"
                     /locus_tag="KPK24_002060"
     CDS             1890..2513
                     /gene="rplD"
                     /locus_tag="KPK24_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG2299732.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2513..2803
                     /gene="rplW"
                     /locus_tag="KPK24_002061"
     CDS             2513..2803
                     /gene="rplW"
                     /locus_tag="KPK24_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG2299733.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2833..3663
                     /gene="rplB"
                     /locus_tag="KPK24_002062"
     CDS             2833..3663
                     /gene="rplB"
                     /locus_tag="KPK24_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG2299734.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3698..3979
                     /gene="rpsS"
                     /locus_tag="KPK24_002063"
     CDS             3698..3979
                     /gene="rpsS"
                     /locus_tag="KPK24_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG2299735.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            4011..4346
                     /gene="rplV"
                     /locus_tag="KPK24_002064"
     CDS             4011..4346
                     /gene="rplV"
                     /locus_tag="KPK24_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG2299736.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            4369..5184
                     /gene="rpsC"
                     /locus_tag="KPK24_002065"
     CDS             4369..5184
                     /gene="rpsC"
                     /locus_tag="KPK24_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG2299737.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            5220..5651
                     /gene="rplP"
                     /locus_tag="KPK24_002066"
     CDS             5220..5651
                     /gene="rplP"
                     /locus_tag="KPK24_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG2299738.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5653..5856
                     /gene="rpmC"
                     /locus_tag="KPK24_002067"
     CDS             5653..5856
                     /gene="rpmC"
                     /locus_tag="KPK24_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG2299739.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5880..6134
                     /gene="rpsQ"
                     /locus_tag="KPK24_002068"
     CDS             5880..6134
                     /gene="rpsQ"
                     /locus_tag="KPK24_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG2299740.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            6160..6528
                     /gene="rplN"
                     /locus_tag="KPK24_002069"
     CDS             6160..6528
                     /gene="rplN"
                     /locus_tag="KPK24_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG2299741.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6552..6857
                     /gene="rplX"
                     /locus_tag="KPK24_002070"
     CDS             6552..6857
                     /gene="rplX"
                     /locus_tag="KPK24_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG2299742.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6887..7429
                     /gene="rplE"
                     /locus_tag="KPK24_002071"
     CDS             6887..7429
                     /gene="rplE"
                     /locus_tag="KPK24_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG2299743.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            7447..7632
                     /locus_tag="KPK24_002072"
     CDS             7447..7632
                     /locus_tag="KPK24_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG2299744.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7665..8063
                     /gene="rpsH"
                     /locus_tag="KPK24_002073"
     CDS             7665..8063
                     /gene="rpsH"
                     /locus_tag="KPK24_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG2299745.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            8094..8636
                     /gene="rplF"
                     /locus_tag="KPK24_002074"
     CDS             8094..8636
                     /gene="rplF"
                     /locus_tag="KPK24_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG2299746.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8672..9040
                     /gene="rplR"
                     /locus_tag="KPK24_002075"
     CDS             8672..9040
                     /gene="rplR"
                     /locus_tag="KPK24_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG2299747.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            9061..9570
                     /gene="rpsE"
                     /locus_tag="KPK24_002076"
     CDS             9061..9570
                     /gene="rpsE"
                     /locus_tag="KPK24_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG2299748.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9585..9770
                     /gene="rpmD"
                     /locus_tag="KPK24_002077"
     CDS             9585..9770
                     /gene="rpmD"
                     /locus_tag="KPK24_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG2299749.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9803..10246
                     /gene="rplO"
                     /locus_tag="KPK24_002078"
     CDS             9803..10246
                     /gene="rplO"
                     /locus_tag="KPK24_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG2299750.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            10290..11558
                     /gene="secY"
                     /locus_tag="KPK24_002079"
     CDS             10290..11558
                     /gene="secY"
                     /locus_tag="KPK24_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG2299751.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11575..12225
                     /locus_tag="KPK24_002080"
     CDS             11575..12225
                     /locus_tag="KPK24_002080"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG2299752.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            12226..12972
                     /gene="map"
                     /locus_tag="KPK24_002081"
     CDS             12226..12972
                     /gene="map"
                     /locus_tag="KPK24_002081"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG2299753.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12994..13263
                     /locus_tag="KPK24_002082"
     CDS             12994..13263
                     /locus_tag="KPK24_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG2299754.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            13301..13519
                     /gene="infA"
                     /locus_tag="KPK24_002083"
     CDS             13301..13519
                     /gene="infA"
                     /locus_tag="KPK24_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG2299755.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13555..13668
                     /gene="rpmJ"
                     /locus_tag="KPK24_002084"
     CDS             13555..13668
                     /gene="rpmJ"
                     /locus_tag="KPK24_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG2299756.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13821..14192
                     /gene="rpsM"
                     /locus_tag="KPK24_002085"
     CDS             13821..14192
                     /gene="rpsM"
                     /locus_tag="KPK24_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG2299757.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            14228..14626
                     /gene="rpsK"
                     /locus_tag="KPK24_002086"
     CDS             14228..14626
                     /gene="rpsK"
                     /locus_tag="KPK24_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG2299758.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14658..15281
                     /gene="rpsD"
                     /locus_tag="KPK24_002087"
     CDS             14658..15281
                     /gene="rpsD"
                     /locus_tag="KPK24_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG2299759.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            15359..16306
                     /locus_tag="KPK24_002088"
     CDS             15359..16306
                     /locus_tag="KPK24_002088"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG2299760.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            16327..16668
                     /gene="rplQ"
                     /locus_tag="KPK24_002089"
     CDS             16327..16668
                     /gene="rplQ"
                     /locus_tag="KPK24_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG2299761.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16788..17621
                     /locus_tag="KPK24_002090"
     CDS             16788..17621
                     /locus_tag="KPK24_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG2299762.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDDLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDGMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17609..18475
                     /locus_tag="KPK24_002091"
     CDS             17609..18475
                     /locus_tag="KPK24_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG2299763.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18469..19272
                     /locus_tag="KPK24_002092"
     CDS             18469..19272
                     /locus_tag="KPK24_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG2299764.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            19304..20035
                     /gene="truA"
                     /locus_tag="KPK24_002093"
     CDS             19304..20035
                     /gene="truA"
                     /locus_tag="KPK24_002093"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG2299765.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            20155..20586
                     /gene="rplM"
                     /locus_tag="KPK24_002094"
     CDS             20155..20586
                     /gene="rplM"
                     /locus_tag="KPK24_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG2299766.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20615..21007
                     /gene="rpsI"
                     /locus_tag="KPK24_002095"
     CDS             20615..21007
                     /gene="rpsI"
                     /locus_tag="KPK24_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG2299767.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21466..22218
                     /gene="cwlD"
                     /locus_tag="KPK24_002096"
     CDS             21466..22218
                     /gene="cwlD"
                     /locus_tag="KPK24_002096"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG2299768.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         9021 a         2625 c         4615 g         6175 t
ORIGIN      
        1 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
       61 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      121 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      181 aagtttacag cagaagtata tgtacttaaa aaagaagagg gtggaagaca tacaccattc
      241 tttgatggat atagaccaca attctatttc agaacaacag acgtaacagg agcttgtaag
      301 ttaccagaag gaatagagat ggtaatgcct ggagataacg taacaatgga agtagactta
      361 ataaactcaa tagttgtaga agagggatta agattctcaa taagagaagg tggaagaaca
      421 gtagcttcag gagttgttgc tacaataata gagtaatcct ttactaaact aatggattta
      481 atataaaaaa aagagggggt aactcctctc ttttttgttt tttgttgaca agtagcgcta
      541 aagcatatat actaaactag tgccttgaga cagattaaca atcaagttta aggttatgat
      601 gttgtaatag aaaataaaat tgaaaaaaac agaaaaaaaa cttgaaaaaa tgtaacagat
      661 gatataaaat ataatagtgt gcttgagaga ttaagaaaat aataattaaa tcctagtgaa
      721 atacgaactg atatcgattt aaaagttaca acacacccgg agcagtgtat cggtattggt
      781 tgtacgcaac ttgaaaatta aacacgtgat aaaggaggga aaacaaaatg gctaagaatg
      841 aaaaaataag aataagatta aaatcatatg atcacaaatt attagatttt tcagcaggta
      901 aaatagttga aactgctaaa aaggctggat cacaagtttc aggacctgtg cctctaccaa
      961 cagaaaaaca agttgtaact atattaagag ctgtacataa gtacaaagac tctagagaac
     1021 aatttgaaat aagaactcat aaaagactaa tcgacatagc taatccaaca cctaagacag
     1081 ttgactcatt aatgagatta gacttaccag caggtgtaga tatagaaata aaattataag
     1141 aacccctaag atattatcct atagatgatt atcttagaat gattactcta aaagatgtaa
     1201 tccgctgtaa gaattatagg aggtgctaat atgaaaggaa tattaggcaa aaaagttggt
     1261 atgactcaaa tattcactga taaaggtgta gttatacctg taacagctgt tgaagcaggg
     1321 cctatggtcg ttactcaaat aaaaacagtt gataaagatg gatacaatgc tatacaaata
     1381 ggttttgaag atgctaaaga aaaagcttta aacaaaccta aaaaaggaca tttagctgct
     1441 gctaatgttt taaagaaaca tttaaaagaa tttagagtag attctgtaga aggatacaca
     1501 gtaggacaag aaataaaagc tgatgtattt gaagcaggtg caaaaataga tgttactgga
     1561 ataagtaaag gtaagggatt ccaaggtcca ataaaaagac atggacaaag tagaggtcct
     1621 gaaactcacg gttctagata ccacagaaga ccaggttcaa tgggagcttg ttcttaccca
     1681 ggtagagtat ttaaaaacaa aaaattagct ggacatatgg gaagcgtaaa ggtaacagtt
     1741 caaaacttag aggttgttaa agtagatgct gacaagaacc ttatattagt taaaggagct
     1801 ataccaggag ctaaaggttc agtagtaact ataaaagaag ctataaaggt ttctaaataa
     1861 tgactaacct agagaaagga ggaataacaa tgccaaaatt aaatgtacta aatgttagtg
     1921 gacaaaacgt tggagaaata gaattatcag attctatatt tggcgtagaa gttaatggac
     1981 atgttttata cgaagttgta aaaaatcaat tagcaaataa gagacaagga actcaatctg
     2041 ctaaaactag agcagaagtt agaggtggcg gaagaaaacc ttggaaacaa aaaggaacag
     2101 gtagagcaag acaaggttct acaagatctg tacaatgggt aggtggagga gttgcttttg
     2161 cacctaaacc aagaagctac aaatatacat tacctaagaa ggtaagaaga ttagctatga
     2221 agagtgcttt atcttcaaaa gttcaaaata gtgaagttat agtattagat gcattaaaca
     2281 tggatgctcc taagactaaa gaatttgctc aaatattaaa caatataaat gctgctaaaa
     2341 aagctttagt agttatagct gacaaaaacg ataacgtaat aaaatcagct agaaatatag
     2401 aaggtgttca aactgcatta gttaatacta tgaatgtata tgatatatta aaatacgatt
     2461 catttataat aactacagat gctgttaaaa aagtggagga ggtgtacgca taatgactaa
     2521 tccacatgat gtaataataa gaccagttgt aactgagcac agtatggctg aaatgggtga
     2581 aaaaaaatac acttttgttg tggctaaaga tgcgaacaaa actgaaataa aaaaagcagt
     2641 agaaaaagta ttcggagtta gtgttgataa agtaaatact ttaaactacg atggaaaagt
     2701 taaaagaatg ggtagaactc aaggaagaac atcaagcttt aagaaagcag tagttaaatt
     2761 aactgctgat agtaaagaaa tagaattctt ccaaggaatg tagtattata acaaacgaag
     2821 gagggaaaac agatggctat aaaaaagttt agaccaactt ctcctgcctt aagacaaatg
     2881 acagttttag tttctgatga aataacatgt aatcaaccag aaaaatctct tttagttaac
     2941 ttaaagaaaa atgctggtag aaacgtacat ggtagaataa ctgttcgtca tagaggtgga
     3001 ggacaaaaaa gaaaatatag aataatagat tttaaaagag ataaagatgg tatacctgct
     3061 aaggttgcta ctatagaata tgatccaaac agaactgcaa atatcgcatt attaaactat
     3121 gctgatggag aaaaaagata tatattagct ccagttggaa ttaacgttgg agatactata
     3181 ttatcaggat taggtgctga tataaaacca ggaaactgtt tagcattaaa agatatgcca
     3241 gttggtacta tcattcataa tatagaatta aagccaggaa aaggagctca attagttaga
     3301 tctgctggag tttctgcaca attaatggct aaagaaggaa aaaatgcttt attaagatta
     3361 ccatcaggtg agatgagatt agtaagcata aactgtaaag ctactatagg tcaagttgga
     3421 aatatagagc atggtaacgt agttatcggt aaagcaggta gaaaaagaca tatgggaata
     3481 agacctactg taagaggttc tgtaatgaac cctaatgacc atccacacgg tggaggggaa
     3541 ggtagatctc caataggtag accatctcct gttactccat ggggtaaacc agctcttgga
     3601 tacaaaacta gaaagaaaaa taaagcttca aataaactaa tagtatcaag aagaactaag
     3661 tagtaaattt atttttgctc ggaaaggagg aatataaatg tcaagatcaa ctaaaaaagg
     3721 gccttttgtc catgcaagac ttttaaagaa gatagaagct atgaatgcta gtggaaataa
     3781 agaagttata aaaacttggt caagatcttc aactgtattt ccacaaatgg tagaaaacac
     3841 tatagctgtt catgatggaa gaaaacatgt tccagtatat ataacagaag atatggttgg
     3901 acataaatta ggtgagttcg ttcctactag aactttcaaa ggacataagg atgacgaaaa
     3961 atctaataag agaaaataat taaattatga ctaagaaagg aggaataaca atggaagcaa
     4021 aagctactgc taaatacgta cgtgtatcac caagaaaagc aggccaaata tgtgaccttg
     4081 ttagaggaaa gaatgttgat gaagcattag caatattaaa gtttactcca agaggagcag
     4141 cttcaataat agctaaggtt gtaaaatcag ctaaagctaa cgcagaaaac aatcacgaaa
     4201 tggatactga aaaattatat atagcatcaa tagttgctaa tcaaggacca acaatgaaga
     4261 gattcatgcc tagagctatg ggtcgtgcaa ctacaataag aaaaagaact tctcatatag
     4321 aggttgttgt taaagaaaaa aaataattaa tagataggag ggaaagtaat gggtcaaaag
     4381 gttaatccac acggcttaag ggtcggtgtt ataaaagatt gggactcaag atggttcgct
     4441 actgataaaa aagagttcgg aaacttatta ttagaagacc ataatatacg taaattctta
     4501 aagaaaagat tatactcagc tggagttgct aagatagaaa tagaaagatc agctaacaaa
     4561 ataaaaatgg acttacacgt tgctaagcct ggtgtagtta taggaagagc tggtgcagga
     4621 atcgaagcat taaaagctga attagaaaaa atgacaaaga aaactataat agttaatata
     4681 gtagaagtaa gaagtacaga taaaaatgct caattagtag ctgaaaatat agctttagct
     4741 atagagagaa gggttgcttt cagaagagca atgaaacaag ctatacaaag agcaatgaaa
     4801 tctggtgcta aagggataaa agtttctgct tcaggaagat taggtggagc tgaaatggct
     4861 agaactgaag gttatagtga aggaaatgtg ccactacaaa ctttaagagc agatatagat
     4921 tatggtttcg ctgaagctga tacaacttat ggaaaaatcg gtataaaagt ttggatatgt
     4981 aatggtgaag ttttaccaac tagagacggt gtaaatccaa gagaagaaag cagaaagagc
     5041 gatagaagag acaacaagag agataataga agaaacgata gaagaggaaa tgacagaaga
     5101 ggaaatgata acagaggaaa ctacagagga caaagacctc aaggtggatc aagacctcaa
     5161 agaactgaaa acaaaggaaa ttaataatag ttaggttcaa ggaaggagga aaaatactca
     5221 tgttaatgcc aaaaagagta aagcgtcgta gagttcatag aggaagcatg gctgggcaag
     5281 ctcataaggg taataaagtt acttatggtg agtttggatt agtagcatta gaagcttctt
     5341 ggataacttc taatcaaata gaagctgcca gaatcgcgat gactagatat ataaaaagag
     5401 ggggaaaggt ttggataaaa atattccctc ataaaccagt aacaagaaaa ccagcagaga
     5461 ctcgtatggg tgcagggaaa ggttctccag aatattgggt agctgtagtt aagccaggaa
     5521 gagttatgtt tgaattagca ggtgtttctg aagataaagc aagagaagct atgagacttg
     5581 ctgcacataa attaccaatc aaatgtaaat ttgttaaaaa agaagattta gaagtaaagg
     5641 gtggtgaata ggatgaaagc taaagaatta agagatttaa caagcgaaga gctaatgaac
     5701 aaattaaacg acttcaaaag tgaattattt agcttaagat tccaattagc tactggtcaa
     5761 ttagaaaata cagctagaat aaagtttgtt aagaaggata tagcaaaagt taaaactgtt
     5821 cttgctgaaa gaaagttata cgaaactaga gcttaatttg gaaaggaggc tttttaaaca
     5881 tggaaagagg aagaagaaaa gttagaatag gccgtgttgt tagtgataag atggataaaa
     5941 caatagtagt agctgttgaa gaattcgtac gtcatccact atataataaa cgtgttaaga
     6001 gaactaagaa attcaaggct catgatgaaa agaatatatg taacatcgga gatagagtta
     6061 aaataatgga aactagacca ttatctaaag ataagagatt cagactagtt gaagttgttg
     6121 agaaagttaa gtagtttttg aagaaggagg gattacgata tgatacaaca agaatcacgt
     6181 ctaagagttg ctgataactc aggagctaaa gaacttttat gtatacgtgt tctaggcgga
     6241 agtaaaagaa gatatggtaa cataggcgac gttatagttg ctactgttaa aagtgcaaca
     6301 cctggtggag ttgtaaaaaa aggtaaggtt gttaaagctg ttatagtaag aagcaaacaa
     6361 ggcgtaagac gtaatgacgg aagctatata tcttttgatg aaaatgctgc tgttataata
     6421 aaggacgata aaactccagt aggaactcgt atattcgggc ctgttgctag agagttaaga
     6481 gacaatgaat tcatgaaaat agtttctctt gctccagaag tactataatt aaggaggtgc
     6541 aataggacat gatgcgtgtt aaaaaaggtg acactgttgt agttatagca ggtaaagata
     6601 aaggtaaaaa aggttcagtt ttaaaggtat atcctaaaac tagcaaagtg ttagttgaag
     6661 gtgtaaatgt aataactaaa catcaaaaac caagtgctat gaaccaacaa ggtggaataa
     6721 taaataaaga agccccaata cacatatcta atgtaatgcc atttgatcct gaaacaggaa
     6781 aaggcgttag agtaagatat gaagtaaaag atgggaacaa agtaagagta tcagcaaaga
     6841 gcggaaaaga attataataa atattgcttg aaaggaggga ccttaaatgg cttctagatt
     6901 acaagaaaaa tacatgaaag aagttgctcc tgctttaatg gagaaatttg gatacaaaaa
     6961 cgtaatggag atacctaagt taaataaaat agttattaac atgggtatag gtgacgctag
     7021 agaaaatcca aaaggattag aaaaaggcgt tgaagaatta gaaatgatat caggacaaaa
     7081 gcctgttata actaaagcta gaaaatctgt tgctaacttc aaattaagag aaggaatgcc
     7141 aataggaaca aaagttacat taagagctga caaaatgttc tactttatgg acaaattagt
     7201 ttcagtttct ttaccaagag ttagagactt tagaggagtt aaccctaatg cttttgatgg
     7261 tagaggaaat tatgctttag gagttaaaga acaattaata ttccctgaaa tagaatatga
     7321 taaaatagat aaagtaagag gaatggatat aatatttgtt actacagcaa aaactgacga
     7381 agaagctcgt gaattattaa aattattagg aatgccattt tctaagtaag caaaggaggg
     7441 attcccgtgg ctagaaaagc gatggttgtt aagcaacaaa gaaagcaaaa gtacgctact
     7501 agagaataca ctagatgtac tatatgcgga agaccgcatt cagttttgaa aaaatttggt
     7561 atatgccgta tatgctttag agaattagct tataaaggtc aaatacctgg tgttagaaaa
     7621 gcaagctggt agaagcttga ttagaatgga aggagggcaa caaaatgaca atgacagatc
     7681 caattgcaga tatgcttact cgtataagaa atgctaatgt tgttaagcat gaaactgtag
     7741 atgttccagc ttctaatatg aagaaagaat tagctagaat cttattagaa gaaggtttca
     7801 taagaggtta tgatgttata gaagatggaa aacaaggtat cataagaata caattaaagt
     7861 acggacaaga aggcgaaaga gttataacag gtcttaagaa aatatctaag cctggaatga
     7921 gagtttacgc tgctaaccat gagataccaa aagtattaaa cggattagga atatcagtta
     7981 tatctacttc aaaaggaata ttaactgaca agcaagctag aaaagaaaat gttggtggag
     8041 aagtaatctg ctacgtttgg taattaatat gaacaatgta aggaggtgcg accatgtcaa
     8101 gaataggtgt taagccaata ataataccag caggtgttga agttactata gctgaaggaa
     8161 atttagttac agtaaaaggt ccaaaaggaa ctttaactaa acaattaagt gctgagttaa
     8221 acataaaaaa agaagaaaat acaataatgg ttgaaagacc aactgataat aaaaaacata
     8281 gatctttaca cggactaact agaactttat tagataatat ggttgtaggt gtaaatactg
     8341 gtttcgaaaa gaaattagaa ttaaaaggtg ttggatatag agctcaaaaa caaggaaaga
     8401 aattagttat gaacttaggt ttctctcatc cagttgagat ggaagatcca gaaggaataa
     8461 ctgttgaagc tccaaaccaa acagagttaa tagtaaaagg tatagacaag caattagtag
     8521 ggaattatgc tgctaagata agagcttgga gaaagccaga gccatacaaa ggtaaaggta
     8581 taaaatacgt tgatgaagta atcagacgta aagaaggaaa aactggtaaa aaataatacc
     8641 aaaacttaaa cgctagaaag gagtgagcta ggtgttaaaa aaggctgata aaaatgctaa
     8701 tagacttcaa agacataaga gagttcgtag aaaaataagc ggtacttctc aaagaccaag
     8761 attatgtgta tttagaagtg ctaacaatat atatgctcaa ataatagatg acacaaaaag
     8821 agtaactcta gttgctgcat cttctttaga agcagaagtt aagagtgctg tcaatcatac
     8881 aggaaacaag gaagcagcta agaaagttgg agaattagtt gctaaaaaag ctgttgaaaa
     8941 aggaatcact gaagttgtat ttgacagagg tggatactta tatcatggaa gaattcaaga
     9001 attagctgaa ggtgctagag aagctggtct taagttctaa tacgaaggag ggaaaaatac
     9061 atgctacgtc gtaagccgat agatgcagga caacttgact tacaagaaaa agttgttgaa
     9121 gtaagacgtg ttactaaggt tgtaaaaggt ggtagaaatt tcagatttgc agcattagta
     9181 gttgttggag atgaaaacgg acacgttggt ataggtgctg ggaaagcaat ggaagtacca
     9241 gacgctataa aaaaagcagt tgaagatgct aagaaaaatc taatagtagt gccaatagtt
     9301 gggactacaa ttcctcacga agtacgcgga cactttggtg ctggaaatat attaataatg
     9361 cctgctgtag aaggtacagg agtaatagct gggggacctg ctagagccgt acttgagcta
     9421 gctggactaa aagacgttag agctaaatct ttagggtcta acaatccaag aaatatggta
     9481 aatgctacaa tagaaggact taattctcta aaaacagttg aagatatagc taaacttaga
     9541 ggtaaaaaag ttgaagaact tctagggtaa ggaggtagtg taaaatggct aaattacaaa
     9601 taaagttagt tagaagtgtt ataggaacta ctcctaacca aaaaaagaat gttgaagcgt
     9661 taggattaag aaaaagagaa caagttgttg taaaagaaga caatgctcaa acaagaggta
     9721 tgataaataa agtaagtcac ttattagaag taactgaaat agctgaataa tttttataaa
     9781 gtagtgcaag gaggtgcaat ccatgaagtt acatgagtta aaacctgctg aaggtgcagt
     9841 aagagctaag agaagattag gtagaggtac tgcaactgga caaggtaaaa ctgcaggacg
     9901 tggacaaaaa ggtcaatggt ctcgttctgg tggtggagta agagtaggat ttgaaggtgg
     9961 acaaatgcca ctagctagaa gacttcctaa gagaggtttc aataacatat ttaagaaagt
    10021 ttacacagaa gttaatgttg aagttttaaa cagatttgaa aatggaacag aaataactgc
    10081 agaattatta aaatctacta aaactataag caaaataggt aaagacggaa ttaaaatctt
    10141 aggtgaaggt aatttagaga aagctttaac tgttaaagct gctaaattta cagcttcagc
    10201 tcaagaaaaa atagaaaaag ctggtggaaa agcagagtta gtataataac ctgctaatct
    10261 cctagccagt acaggcgggg tgaattagcg tgctgtcaaa attaaaacaa gcttggaaga
    10321 ttaaagcagt aagaaaaaaa gtaatgtata cacttatgat gatcgtgata ttcagaatag
    10381 gtactactat accagttcca ggcatagaca ctagtattat acaaaagatg gtaggcggca
    10441 atagtctact ttctctgtat aatatgttta ctggtggagc atttagtaac ttttcactat
    10501 ttgctttagg tataagtcca tatatcactg catcaatcat aatacaactt ctaacagtag
    10561 gttttgaaag tttagcagaa cttcaaaaat ctggtgaaga aggaaagaaa aaaatcaata
    10621 agtacactaa atatacagca ctagctctag cagttgtaca agctttggga attacattag
    10681 gaattgtaag aagtgcttta atatcaaata gtgtgttttt tatcacaact gttgtaataa
    10741 cattagtttc agcaagtatg ttagtaatgt ggattgggga caaaattact gaaaaaggaa
    10801 tcggtaatgg tagttcagta ataatatttg caggtataat atcaagaata cctacagatg
    10861 ttattaaaat atcgcaacaa gttaaatctg gcgaagttgc accttgggtt atagtgattc
    10921 tagctgtagt tatattactt acagtaacag gagttacatt tatacaagaa gcaactagaa
    10981 agatacctgt acaatatgct aaaagagttg ttggaagaaa aatgtatgga ggacaaagtt
    11041 ctcatatacc aatgaaagtt aaccagtcag gagttatgcc tataattttt gctagttcac
    11101 ttttagcttt ccctcaaaca atagctatgt ttatgggtcc aaatgctcag gcttttgttc
    11161 aaaaatactt aagtatggca acagaacaag gattttggac atatagatca attgagattc
    11221 tacttataat tttcttctca tatttttata caactgtatc atttaataca gaagacatat
    11281 ctaaaaatat gaaaaataat ggtggattta taccaggtat aagaccaggt gagcctacta
    11341 tggattattt aaatagaatt ttaactagat taactttagc tggagctact ttcttagcaa
    11401 taattgctat ggtgccagct cttacaacac attatatgaa agtaaatatg agtctagctg
    11461 ggacttcatt gctaatcgtt gttggagttg ctttggagct taagagacag ttagagtcaa
    11521 acttagttat gagaagctat caaggtttct taaaataaaa tggagatgga taatatgaga
    11581 ataatattac ttggacctcc aggtgctggt aaaggtactc aggcggcagg aatagtagaa
    11641 aaatacaata tacctcatat atcaacagga gatatattca gaaagaatat aaaagaggga
    11701 acagaacttg gaaaaaaagc taaagaatac atggaccaag gtttattagt accagatgag
    11761 ttaactgtag gtttagttac tgatagaata tctcaagaag attgtaaaaa tggatttatg
    11821 ttagatggat ttccaagaaa tgtagcacaa ggagaacatt tagatatctt cttaaaaaat
    11881 gctggtatat cactagataa agttgtcaat attgaagttg ataagagtat attagtgtct
    11941 agagcagttg gtagaagaat atgtaagtct tgtggagcta cttaccatgt tgagtttaat
    12001 cctcctaaag tagaaggtgt atgtgatgta tgccaaggag aattatatca aagagctgat
    12061 gataatgaag aaactgtatc taagagaata caagtttatc tagatgaaac taagccttta
    12121 gtagattatt atagcaaaca aggtataata gcagatataa aaggtgatca agcaatagat
    12181 aaagtatttg aagatattgt cgcagcctta ggaagtggaa aataaatgat tatcttgaaa
    12241 tcaaaaaaag aaatagaact tttaagagaa gcaggtaaaa tagttgctga cactcatgaa
    12301 gttttaagaa aagctatttc tccgggaata tctactttag aattagataa aatagctgaa
    12361 gagaatataa gaaaatacaa tgcagaacct tcttttaaag gatatggagg ttttccagga
    12421 agtatatgtg cttctatcaa tagagaggta gtacatggta ttccaggaga aactatatta
    12481 caagaaggtg acattgtaag tttggatata ggagcctatt ataaaggata tcatggtgat
    12541 tctgcgaaaa ctcacggagt aggcatgata tctgaagaag ataggaaatt gatagaagta
    12601 actagagaaa gcttctatga aggaataaaa tttgctaaat taggatatag actttcagat
    12661 atctcacacg cagtacaaac acacgttgag aaacatggtt tctcggtagt tagagattta
    12721 gtgggtcatg gagttggagc aaatcttcat gaagacccac aagtacctaa ttatggactc
    12781 ccaggaaaag gaccaagact tagagaagga atggttattg ctatagagcc aatggttaat
    12841 gcaggtcgtt atcatgtaaa gactctatca gatgggtgga cgactgttac aatagatgga
    12901 aaaaaatcag ctcattatga gcatacgata gcaattactg aacatgaacc tttgatattg
    12961 acaaagttgt aatttaaaaa gtaggtgaaa aaagtgctat cagataattt aagtataggt
    13021 caagttgtta aagtttcatt aggacgagat aaaggaaatt tattctttgt agtcaaaata
    13081 attaataatg aatatgtatt aatagctgat ggaaaaaaga gaaaacttga caaacctaag
    13141 ttaaagaaag tgaagcactt aaaaaaatat aattttatta atgatgaagt tagaaaaaga
    13201 gtagtatctg gacaagagat tacagattct tttttaagag ctgaacttac taagttaaat
    13261 tagataattt aacttattga aatggagggc tggttagtta atggccaaaa aagatgttat
    13321 agaattagaa ggtacagttt cagaagcttt acctaatgct atgttcaaag ttaaactaga
    13381 aaatggacat gagatattat gccacatttc tggaaagcta agaatgaact tcataagaat
    13441 tcttgaaggt gacaaggtga atgttgaact ttctccatat gaccttacaa gaggaagaat
    13501 tacttggcgt aagaagtaga ctatattaat atttagtcta aggagggatt aataatgaag
    13561 gttagaccat cagtaaaacc aatatgtgaa aaatgtaaag taataaaaag aaaaggaaaa
    13621 gtaatggtta tctgtgaaaa tccaaagcat aagcaaaagc aaggataaga aattattact
    13681 agcattttgt taaattttat agtataatac tatattgtga tgttaacaaa agaacgttaa
    13741 aatcgttgaa caatctggcg taggattggc atatgatgtg aagttttaaa cactaaaaac
    13801 tatgtgtagg aggtgcgatc atggcaagaa tagctggggt agatttacct agagaaaaaa
    13861 gagcggagat aggcttaact tatatatatg gtataggtaa agcgactgct aacgaaatat
    13921 tagctaaggc tgagataaat ccagatacta gaataaaaga tttatcagaa gatcaagtta
    13981 atgagttaag aaaagttata gatgacgatt tcttagttga aggtgactta agaagagaaa
    14041 ttgctttaaa cataaagaga ttaagagata taaaatgtta tagaggaata agacacgcta
    14101 aaggtcttcc attaagagga caaagaacta agactaatgc tagaactaga aaaggtccta
    14161 gaaaaacagt atctcgtaaa aagaaaaaat aaataaataa taactagatg aaggagggaa
    14221 aaggaaaatg gctaaaccaa aaaagaaagt tacacgtatt agaagaagag aacgtaaaaa
    14281 tatagaacgt ggtcatgctc atatacaatc aactttcaat aatacaataa taactttaac
    14341 tgacgttcac ggaaatgcta tatcttgggc aagttctgga caattaggat tcaaaggatc
    14401 aagaaaatca actccatttg catctcaaat ggctgctgaa acagctgcaa aagctgcgat
    14461 ggaacacgga ctaaaaagtg ttgaggtatt cgtaaagggg ccaggttcag gaagagaagc
    14521 tgcaataaga gctttacaag caactggact agaagtaact atgataaaag acgttactcc
    14581 aatcccacat aacggatgca gaccaccaaa gagaagaaga gtgtaattaa taagagtaca
    14641 ataggaggtg tagataaatg gcaagatata caggtgcatc atgtagacaa tgccgtagag
    14701 agggaatgaa attattcctt aaaggtgata gatgttatac agataaatgt gctatagtaa
    14761 aaagaaacta tgctccaggt caacatggcc aaggaagaaa gaaagtttct aactatggat
    14821 tacaattaag agaaaaacaa aaagttaaaa gaatatatgg agttttagaa actcaattta
    14881 gaaatttata tgaacgtgct gaaaatatgc ctggtaaagc aggtgagaac ttattaagtt
    14941 tattagagag aagattagat aatgtagttt acagaatggg actagcatct tctagaaaag
    15001 aagctagaca attagtgact catggtcatt tcactttaaa tggaaataaa gtagacatac
    15061 cttcattaat agttaaggtt ggagatgtta tagaagttaa agagaaatca agatcttctg
    15121 caaaattcaa aaatttagta gaagttaact caagaatagc tcctaagtgg ttagaagcta
    15181 atgtagaagg aatgacagca aaagtagttg gtgttccaac aagagaagat atagatcttg
    15241 agatagcaga gcacttaatc atagaacttt actctaaata ataagcacac ttttaaaaat
    15301 tattttaaaa ttttgtttac cctcagtgga ttactgaatt ttaaggaggg ttttgtccat
    15361 gatagaaata gaaaagccaa aagtagatat agttgagctt agcgaagact atagatatgg
    15421 taagtttgtt atagagcctc tagaaagagg atatggaata actataggta acgcattaag
    15481 aagaatatta ttatcatcat taccaggtgt agcagtgaat gctataaaaa tagatggagt
    15541 tcttcatgaa ttttcaacaa tacctggtgt taaagaagat gttactgaga taatattaac
    15601 tttaaaagag ctttcagcaa ctatagatgg tgaaggaagt agaacactta aaatagaagc
    15661 tcaaggacca tgctctatca caggtgcaga tataatctgt cctccagatg ttgaaatatt
    15721 aagtaaagat ttagcaatag ctacattaga tgataatgct aaacttaata tggagatatt
    15781 tgtagataaa ggtagaggtt atgtttctgc tgaagaaaat aaaacagaga atgttccaat
    15841 aggtgtttta cctgtggatt caatatatac tcctgttgag aaggttagtt atcatgtgga
    15901 aaacacaaga gttggtcaaa aaacagatta tgataaatta gtactagaag tttggactaa
    15961 tggtagtata aatcctcaag agggcatatc attagctgca aaggtattag ttgagcattt
    16021 aaatttattt atagacttaa ctgaacatgt aagtagtgtt gaaatcatgg tagaaaaaga
    16081 agaagatcaa aaagaaaaag ttcttgaaat gactatagaa gaattagatt tatcagttag
    16141 atcatataac tgtttaaaga gagcgggaat taatacagtt gaagaattag ctaataaatc
    16201 tgaggatgat atgatgaagg ttagaaacct aggtaagaag tcattagaag aggtaataca
    16261 aaaactagaa gaacttggat tgggattaaa accaagtgaa gaatagcgag caaaggaggg
    16321 ataagaatgg ctaagtaccg taaattagga cgtgaaacag ctcacagaaa ccttatgtta
    16381 agaaacttag taacttgctt actaagaagt ggaagaatag aaactacagt gactagagcg
    16441 aaagaaactc gtagaatggc tgaaaagatg ataactcttg ctaagagagg ggatcttcat
    16501 gctagaagac aagttttagc ttatgttatg gatgaaacag tagttaataa cttattcaca
    16561 gatttagctc caaaatatgc tgagagaaat ggtggataca ctagaataat aaaaataggg
    16621 ccaagaaaag gcgatgctgc tgaaatggct tttatagaat tagtatagta aggaaaggat
    16681 taggcttgat ggcttaatct tttttttatt tttttgacta aggtgtgata taataatgtg
    16741 taaaatgatt tttataagtt attgcttact aaaaggagta tgagttaatg gataatatag
    16801 taaaggtaaa taatatttcg tttgagtata ttacagatga agcaaaactt aaagcaatag
    16861 atgatttaag cctagatgtt aaaaaaggag aatttgttgc aataatagga cataatggtt
    16921 ctggtaagtc tactttatct aaaaacttaa atgctattct tatgcccact gaaggaaata
    16981 ttcttattga tggtatggac actaaagaag aagaaagatt atgggatata agacaaactg
    17041 caggcatggt atttcaaaat ccagataatc aaatagttgc tactatagta gaagaagacg
    17101 ttgcatttgg acctgagaac ttaggtatag aaccaaaaga aataagacga attgtagaag
    17161 agtctttaaa aagtgtaggt atgtatgatt taagagatag acagcctcac ttgttatcag
    17221 gaggtcaaaa gcaaagagtt gcaatagcag gaattattgc tatgaggcct aaatgtataa
    17281 tatttgatga agcaacagca atgttagatc catctgggag aaaagaagtt atgaaaacaa
    17341 taaaaagact taataaggaa gaaaatataa ctgttataca tataacgcac tttatggaag
    17401 aagcagtaga agctgataga gttgtagtta tggaaaaagg aaagaaaatc ttagagggaa
    17461 ctcctagaga agtattcagt aaaataaaga tgttaaagga aatcggttta gatgtacctt
    17521 gtatgacaga gttatctagt ttattaatag aagaaggtat aaatattagt agtgatatat
    17581 taactgtgga tgagatggtg atggaattat gtcaattata gtaaagaatt taacacatat
    17641 atataatgaa gggatgcctt ttgcaagtaa ggcacttgat gatgtttctt ttgagattaa
    17701 agatagagat tttgttggtc ttataggtca tacaggctca ggaaaatcta cattaataca
    17761 acatttaaat ggattactaa agccttcctc aggagaaata tttataaatg attttaatat
    17821 aacagataaa aacttgaatt taactgagat tagaaaaaga gtaggggttg tatttcaata
    17881 tccagaatac caattgtttg aagaaacgat tgataaagat atagcttttg gaccctctaa
    17941 tttaggctta gaggagtctg agatacataa cagggtaaaa gcatcaatgg aagctgtagg
    18001 acttgattat gagggattta aagacaaatc tccttttgaa ttatctgggg gacaaaagcg
    18061 tagagtagca attgctggag ttatagccat gaacccagaa gttctcatat tggatgaacc
    18121 aactgctggt cttgaccctg gtggaagaga tgaaattttt aacttaataa aagacttaca
    18181 tgaaaaaaag aatatgacta taatactatc atcacatagt atggatgata tggcaaagtt
    18241 agcaaaaact ttaattgtta tgaatcatgg tagcgtagaa tttatgggaa ctcctagaga
    18301 agtgtttaaa tcaaatgcta gtaaattaaa agatatagga ttagatattc cacaagtttt
    18361 agaacttgca ttgaaattga gagaaaaagg ctttgacatc agtgaggata tattgacttt
    18421 agaagaagca aaacaggaaa tattaaaagt tgtgagagga cgaggattat gttaaaagat
    18481 ataactatag ggcaatacta cccaacaagt tctgctattc ataaattaga tccaaggata
    18541 aagcttgtag cgacaattgt atttatggta tctatatttg tagtaaataa attttggcct
    18601 tatatagtag ttttattatg tttattagct atgataaaat tggctaatat accagtcaaa
    18661 tatattgtta agggtgttaa acctcttaaa tggattattt tgtttacatt tcttataaat
    18721 atttttttct taccaggtga tgaaatatgg tcatttggat ttttagctat tacaaaacag
    18781 ggtctaagac aagcaatttt tatggcaata agattaatat ttttagtagt tggtacatca
    18841 ttgcttactt taacaacttc accaatagaa ttaactgatg gaatagagag attgttgaat
    18901 ccatttaaga aaattggttt gccagttcat gaacttgcaa tgatgatgac aattgcttta
    18961 cgttttattc ctacattgtt agatgagaca gacaaaataa tgaaggctca gatgtctaga
    19021 ggtgcagatt ttgaaagtaa gaatcttata aatagagcaa agaatttagt accacttttg
    19081 gtacctttat ttgtaagtgc atttagaaga gctgatgaat tggctatggc catggaggcc
    19141 agatgttaca gaggtggaca taataggact aagatgagag agtctgttat atcaagaagg
    19201 gattatatgg catgtgtatt tcaggttgtt tatctgggag caattatagc tactagattt
    19261 atagcaattt aattaataag ttatagatat aatgaggtaa agtatgagaa atataaaaat
    19321 taaaatccaa tacaatggta agaattattg tggatggcaa aagcaacctg attctttagg
    19381 gatacaaggt acaattgaaa gggctatata tgatataaca aaagaagaaa ccagtcttat
    19441 agggtctggt agaactgatt caggagttca tgctattggt cagattgcaa attttaaaat
    19501 taattcaggt ataagtatag aaagtatacc aatggcacta aatgccaagt tgccaaaaga
    19561 catatcagtt atagaagcat gtgaagtaaa tgatgatttt cactccagat acagtgcaaa
    19621 aggtaaaaca tataaatatt tagtatataa tagcaagttt agaaatccaa tacttagtga
    19681 gatatcatat caagtaaaat atgaacttga ttttgataaa atgtgttcag aagcaaaaag
    19741 tttattagga acacatgatt ttaaaggttt tatgagttct ggttcatctg taaaagatac
    19801 agttagaacg atatatgata ttgatataag taaaaaagat gacttgatta cttttgagat
    19861 tagtggtaat ggttttttat ataacatggt gaggattatt gttggaacac ttgttgatat
    19921 gggtcgtggc agaattaacg aaccattttt agatataata caatcaaaaa ctaggtcaag
    19981 atgtggacat acagctccag cacaaggatt atttctcaaa aaagttcatt attaacttga
    20041 cacaccaggc tacatgtatt aaaatatatg agaatgtgtt aataagtcca cttgccccgg
    20101 acttgacata aacggtaaaa aaattttatg aaaagtagtt aaggagggac agttatgaaa
    20161 agttatatag ctaagccagc tgatgtacaa agaaaatggt acttagttga tgctgaagga
    20221 aaaacattag gtcgtttagc aacagaaatt gcgacagtat tgagaggtaa acataaacca
    20281 acatttactc ctcacgttga tggtggagat tttgttgttg tagtaaatgc agaaaaaata
    20341 gttttaagtg gtaagaaatt agatcaaaaa tattacagat accatactgg ttatgtaggt
    20401 ggattaaaag aaatatctta cagagatatg atggataaga aaccagaaga agttatatca
    20461 catgctgtaa gcggtatgtt acctaaaaat aaattaagaa gcagaatgat gactagatta
    20521 agagtatttg caggtgctga gcacactcat gcagctcaaa atccagaagt tttaaacttt
    20581 aaataagatt aataggtgaa aggaggagtt tataatggct aacgttcaat attatggaac
    20641 tggaagaaga aaaagttctg ttgctagagt aagactagtt gcaggtgaag gaaatatatt
    20701 agtaaatgga agagcattag aaaattattt taattatgag acattaataa gagatgttaa
    20761 acaaccatta gttttaactg gtaatgaaaa taaatatgat gttatagtaa aagttgaagg
    20821 tggaggattc actggacaag ctggagctat aagacatgga atatctagag ctttattaaa
    20881 agctgattta gatttaagac ctgctttaaa gaaagaaggt ttcttaacta gagatgcaag
    20941 aatgaaggaa agaaagaaat acggattaaa agcagcaaga agagctccac aattctcaaa
    21001 aagataatta cttatttttt tggaaaagca aaatccttta tttactgtta ttcagaaagt
    21061 attggtattt taaaaatgtt gggttaagtt aattattgat atataataaa ataataagat
    21121 atcttattag gatgtttttc attaagaaga acatcctttt tattttgact atttaataaa
    21181 aaacatatgc taataagatt taaattttat ttttataata ttgtaattta aaagtttata
    21241 taaggttata tattttaaga tattatttaa caatataaat tctgaatata cgttatcaaa
    21301 gtttataata taaactaagt ataatttatt gtatagtatt gaatgttaga taaacatagt
    21361 gaattaaata ggggcagaaa tataatatca ttacataata atatattaat accaataaat
    21421 aaagatatat caatattaaa aaaatttaaa tgtttgtact aatatatgga atattatagt
    21481 gtttttataa gtatacatat acagggtgat gttgtgagaa agtacataaa acatataatt
    21541 tttagttttg caatgatatg tctagtagta gtatccatat ttgaaattaa aaatatttct
    21601 gaagatgtta tcaagtatat gccagtaaca aacaaaacta taattttaga tgctggtcat
    21661 ggaggcattg atccaggtgc attaaataag gataagagta catctgaaaa ggatattaac
    21721 ctagcaataa cacttaagct tagagagctt atagaatcaa gtggtggcct tgtaatatta
    21781 actcgagaag atgacagtag cctttataaa gaagaaaata ataaaactac aagacaaaaa
    21841 tataatgaga atttgaaaaa tagaaaagaa ataatatcta attctaatgc caatatgttt
    21901 gtctctatac atcttaatgc atttgaacaa tcaaaatact atggagctca aactttttat
    21961 cctaaagaca aacaagacag taaagaatta tcgaagtgta ttcaagaaga acttaaaaga
    22021 gtagtagata agacaaacaa cagagaagtc aaaccaaggg atgatatata tcttttgaaa
    22081 gaaaataata ttccatcagt actgatagaa tgtggttttt tatcaaatga aaaagagtgt
    22141 aaactcttaa ctgatgaaac atatcaagaa aaaatagcat gggcaatcta cataggaata
    22201 caaaaatatt taagttgaaa taactataaa aataggtcta aatatgtaaa tagcttgtat
    22261 taaagcagtt aaaatgcatt aatataggct atttttattt tgacaaaaaa atatttaaaa
    22321 taaaagttaa aaagttgttg acttagaata atatagatga tattatatat gagtgcccaa
    22381 aaggagcacc aaaataagac aaaagaactt tgaaaattaa acagtaggtt aattta
//
LOCUS       DAEMGG010000058        22086 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEMGG010000058 DAEMGG010000000
VERSION     DAEMGG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.058"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            73..249
                     /locus_tag="KPK24_002097"
     CDS             73..249
                     /locus_tag="KPK24_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299769.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            423..1553
                     /locus_tag="KPK24_002098"
     CDS             423..1553
                     /locus_tag="KPK24_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG2299770.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            1794..2144
                     /locus_tag="KPK24_002099"
     CDS             1794..2144
                     /locus_tag="KPK24_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG2299771.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2152..2601
                     /locus_tag="KPK24_002100"
     CDS             2152..2601
                     /locus_tag="KPK24_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBG2299772.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            2703..3278
                     /locus_tag="KPK24_002101"
     CDS             2703..3278
                     /locus_tag="KPK24_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG2299773.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3271..4680
                     /locus_tag="KPK24_002102"
     CDS             3271..4680
                     /locus_tag="KPK24_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBG2299774.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            4864..6297
                     /gene="eutA"
                     /locus_tag="KPK24_002103"
     CDS             4864..6297
                     /gene="eutA"
                     /locus_tag="KPK24_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBG2299775.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6315..7679
                     /locus_tag="KPK24_002104"
     CDS             6315..7679
                     /locus_tag="KPK24_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBG2299776.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            7692..8573
                     /gene="eutC"
                     /locus_tag="KPK24_002105"
     CDS             7692..8573
                     /gene="eutC"
                     /locus_tag="KPK24_002105"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBG2299777.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8593..9246
                     /gene="eutL"
                     /locus_tag="KPK24_002106"
     CDS             8593..9246
                     /gene="eutL"
                     /locus_tag="KPK24_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBG2299778.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9257..9958
                     /locus_tag="KPK24_002107"
     CDS             9257..9958
                     /locus_tag="KPK24_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG2299779.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            9961..11430
                     /locus_tag="KPK24_002108"
     CDS             9961..11430
                     /locus_tag="KPK24_002108"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBG2299780.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11526..11813
                     /gene="eutM"
                     /locus_tag="KPK24_002109"
     CDS             11526..11813
                     /gene="eutM"
                     /locus_tag="KPK24_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBG2299781.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            11940..12701
                     /locus_tag="KPK24_002110"
     CDS             11940..12701
                     /locus_tag="KPK24_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBG2299782.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12714..13343
                     /locus_tag="KPK24_002111"
     CDS             12714..13343
                     /locus_tag="KPK24_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG2299783.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13385..14110
                     /locus_tag="KPK24_002112"
     CDS             13385..14110
                     /locus_tag="KPK24_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBG2299784.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            14123..14395
                     /locus_tag="KPK24_002113"
     CDS             14123..14395
                     /locus_tag="KPK24_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBG2299785.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14388..14939
                     /locus_tag="KPK24_002114"
     CDS             14388..14939
                     /locus_tag="KPK24_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG2299786.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGENSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQNLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            14951..16042
                     /gene="eutH"
                     /locus_tag="KPK24_002115"
     CDS             14951..16042
                     /gene="eutH"
                     /locus_tag="KPK24_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG2299787.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            16044..16517
                     /locus_tag="KPK24_002116"
     CDS             16044..16517
                     /locus_tag="KPK24_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBG2299788.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            17030..17551
                     /locus_tag="KPK24_002117"
     CDS             17030..17551
                     /locus_tag="KPK24_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2299789.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            17930..19105
                     /locus_tag="KPK24_002118"
     CDS             17930..19105
                     /locus_tag="KPK24_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2299790.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDILKLGCEFMAKYAVEFLNKNNN"
     gene            19139..20677
                     /locus_tag="KPK24_002119"
     CDS             19139..20677
                     /locus_tag="KPK24_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG2299791.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            20709..21911
                     /locus_tag="KPK24_002120"
     CDS             20709..21911
                     /locus_tag="KPK24_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2299792.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
BASE COUNT         8957 a         2483 c         4116 g         6530 t
ORIGIN      
        1 gaaatttaaa tattttaaaa gataattttt ttgaactaat aggtataaca aaaatttata
       61 gggggattgc atatggaaat caatgttata gaaattttcc ctaaagataa agctaaactt
      121 aataaaatag aaatggataa agctagttgg tttgtaaata taataagtaa aaaatatcct
      181 aaagaagctt taaatgaagc atttagtact ttagaaaaag aattaaatat aagtaaagct
      241 aatacataag ctttgattat tgattttata tactattggt tttatacgct atgacttgac
      301 attatattaa tcaaatagta tatttttaaa tatagcacaa tgaagtgtta aaagagcaat
      361 ggagctccat agataaagtc tatggagctt ttttgcgtat ttaacttaag gaggatagga
      421 ctttgaataa ctttgagatt aaaactagaa taaaatttgg agaaggctca ctaaaagctt
      481 tgcaagaatt aaaaaacaaa aatgtactaa taataacaga cccttttatg gtgaaatcaa
      541 aaacaataga taaaataaca tctaatttaa taaactcaac atatcaagtt tttgaagaaa
      601 tagtaccaga cccaccaata gaagtagtag tagcaggaat agaagcattt aaatctataa
      661 atccagacgt aataatagca ttaggtggtg gttctgcaat agatgcagca aaatctataa
      721 tggacttttc taaaaaaata ttaaaaatta acgaagtaga gtttatagca ataccaacaa
      781 caagtggaac aggttcagaa gtaacatcat ttgctgtaat aacagatagt caaaaaaaat
      841 gtaagtatcc attagtgagt gatgatttac ttccagatat ggcaatacta gacccagaac
      901 ttgtaaaaac tgtaccaaat tttataacag cagatacagg gatggatgta ttgacacatg
      961 ctatagaagc ttacgtatct acaaattcta atgacttttc agatgcacta gctgaaaaag
     1021 ctataaagtt agtatttaaa tacttaataa aagcatataa agatggaaat gacatagaag
     1081 ctagagaaaa aatgcacaat gcatcttgtt tagcaggtat ggcatttaac caagcatcac
     1141 ttggcataaa tcattctatt gcacatgtat taggtggaaa attccatata ccacatggaa
     1201 gaacaaactc tatattactt cctcatgtaa tagaatataa ttcatcaata gtaggatata
     1261 ctaacacaac ttactctgct acagctaaaa aatatgcaca aatagcagaa ttagtaggac
     1321 ttgggagtgc taatataaga atatctgtta gaaatttagt aaatgaaata aagaaactac
     1381 aaaaagaaat gaatatgcca actaaattga cacaatgcaa attaagttta gaagatataa
     1441 ctaatgaaga aagagaaata gcaaaacttg ctataaaaga tacttgtaca ttaactaatc
     1501 ctaaggtacc aactgaagat gatataataa acatattaaa gaacattaaa taactacatt
     1561 ttattacatg taaatataat attatacaaa tagggaaaat gtttttaaaa atataaatta
     1621 tatattgaca aatataaaac ataaaactat aatgaaagta tcacaaaggc gtgatacttt
     1681 agtagaaagc aatggagctt cttaggaata ttaatattat tcttatggag ctccttttat
     1741 tttaatcaaa tagcttttta ataaactata ttcaaagaaa gcagggaata aatttgactg
     1801 aagaatctaa gcaaagagtt attcaagaat atgttcctgg aaaacaggtt acattagcac
     1861 atatcatagc taaccctaat gaagatatat ataagaagtt aggattagta cttgataaaa
     1921 aagatgcaat aggaatactt acaataacac caagtgaagc gtcaataata gcagcagatg
     1981 ttgctacaaa ggcttcaaat gtatctttag gttttataga tagatttagt ggttcagtag
     2041 taatatcagg tgatgtaagt tctgtagaat cagcgttaaa tgatgtttta gaagtcttag
     2101 gaaatatgtt aaacttctca tctacaaaaa tcacaaggac ataataatac aatgaagaat
     2161 ataatattta tgggaaagac aggtagtggt aaaactacct tatgtcaaaa actagaccag
     2221 ttagaattaa agtataaaaa aactcaatca gtggaacttt ataataatgc aatagacact
     2281 cctggtgagt atatggaaaa tagaaaccta tataatgcct taataacaac atcagcagat
     2341 gctaaaatta tagcaatagt gtatgatgcc acacaagaag aaaattatat agcaccaggt
     2401 tttgccagta tgttttgcaa agacgtaata ggaattataa ctaaaataaa taaagctact
     2461 gaaaacgaag tcaatatagc ttatgaaaga ctatcactag caggagcaag tcaaatcttt
     2521 aaaatagaca cagttgataa tattggactt gaaaaattat ttaaatatct agaaaaatat
     2581 aaaaatatag atgaagttta aggggaaata tttttttatt caaaaatatt attaaagtat
     2641 gataaaataa aaaaatgtat aaactatttt aagaagattt aatctttaag cgaggtagga
     2701 acatggggaa aaaaatacta ttggttgatg atgaaccact gttaaggatg gatacaaaag
     2761 atatattaga aagtcatggc tatgaaataa taggcgaagc aagtgatgga tttgaagctg
     2821 ttgaaatgtg taaaaaacac aaacctgatt tagttataat ggatatagag atgccaatac
     2881 ttgatggtat aaaggcaggc aaaataatat gtaaagaaaa tatagctggt ggagtacttt
     2941 tattaacttc atttgaagat aaagaatacg tagaaaaggc taaaagtata ggtgcttatg
     3001 gatatttagt aaaaacagca agtgaaaaat cacttatccc aaccatagaa atgtgtttaa
     3061 ataaggtaaa tgaatttgaa aaaatgaaaa aagatttaga aaaagtaaat tctaaattga
     3121 atgaaagaaa attagtagaa agagcaaaag gtatacttat aagagagttt aagatttctg
     3181 aggatgaagc atacactaga ataagaaaat taagtatgga taaaagaact acaatgatgg
     3241 aaattgccaa aatgattata ataggatacg atgactaata cgataaaaaa aatatcaact
     3301 gcacaaacat acttaaataa taacgaaatt gaaaaaatta tagaagtatc aaaatcctta
     3361 gatttaatgg cttcttttta tgaagctgat gtttttatag atgtactaag taaatttgat
     3421 gatgaagcaa ttgttgtggc acatggtaga cctaaagaaa agtcaatgta ctgtgaaaat
     3481 gttataggta aaaaggcttt aattgaaaat gaaccaggag ttataaagtg cttaaaaaca
     3541 ggagaaagag taagagatat taaagcttta acacaggaat ataaactggt caaacaaaat
     3601 attcagccaa taacactaga tgacaaagtt ttagcagtac taatagttga aaaagatata
     3661 agtagtgaag ttaagaatga gtttaatgct agtgaagacg aagaaaataa aaatttacat
     3721 cagtttataa aacttattgg aaataatgac tttttagttg ataatttaaa ttcagccata
     3781 ttaatatttg ataaaaatgg aaaattaaaa attaaaaatc gtattgcaac tgagatatac
     3841 aaaaatcttg ggtttggata tgatatacaa gatatgcatt acaatgattt agatatagaa
     3901 accaacacct ttgaaaatat attaatggat gaaaattatg atgagacaaa tgaagtttca
     3961 ataggtaata tgttttttaa aataaagacc atatacatca aagatgatga atttaaggtt
     4021 tgcaaaatag tacaagatat aagtgattta aagagaaaag aagaagaatt agttttgaaa
     4081 acagtggcta taagagaagc tcatcataga gtaaaaaata atcttcatac agtaatatct
     4141 ataataaaaa aacaaagtag attatctcaa aatgatgaag taaagcagtg tttagataac
     4201 ataataaata gggtatttgc aatactttcg actcattatt tactatctaa agaagttgat
     4261 aataatatat ctatacgaga aggtatcaat ttattagtgt caaatattca aggtggctat
     4321 ttatttagta aggatataga tatacgtata actggtgaag attttaaaat tagtggagaa
     4381 aaagcaacag caatactttt ggttataaat gaaatactac aaaattgtta tgaccatgca
     4441 ttttcggata gagaacatgg tactatacaa atattaataa ataaagataa cgactataga
     4501 aatattgcta ttatagatga tggaattgga tttgatagta ataatataaa taaaaatagc
     4561 ttagggacat atataataga tagttatata acacagatgt taaaaggaaa tatagagaga
     4621 aaaagtagtg agaatggtac agaagtatta attactatac ctacacaaat taatatttaa
     4681 ataataactc atctacaaag tagtagtttt aattgagcaa agaggcttaa ggttattaat
     4741 agattaagtt aattgaatgt atttataaaa attataagta tatattcaat ttaacaggta
     4801 tttattataa ctttaagcct ctttgcttgt ttaagacata cttaaatttt aggaggtgag
     4861 aaaatgagag aagagttatt aagtgttggt attgacattg gaacaagtac tactcagtta
     4921 attttttcta aattaatagt agaaaatact gaatcaaatt tttcggtacc tagaataagt
     4981 atagtagaca aagaaattat ttataaaagc aaagtttatt tcacccctct tatttcaaat
     5041 aatgaaataa atggtcaaca aataaaagat atagttgaaa atgaatataa aaaagcaaat
     5101 atatctaaag aagatataca tacaggagca gtaataataa ctggtgaaac agctagaaaa
     5161 gaaaatgcaa atgatgtact tcatactttg agtggatttg ctggggactt cgttgttgca
     5221 acagcaggac ctgatttaga aagtataata tcaggaaaag gagctggagc tcatatttat
     5281 tcaaaagaac attcaactac aattgtaaat atagacatag gtgggggaac tagtaacata
     5341 gcctcattta aaaggggaga agtaaccgat actgggtgtt tggatatagg tggaaggctt
     5401 gtaaagatag ataaaataag taaaaaaata acttatatat cacctaaaat agataaaatc
     5461 ataaaagata ataatttaaa tcttcaagta ggtacaatgg ctactgtaga aaatacaaag
     5521 ccaatagtgg atataatggt tgacttatta atggaaagca taggactaaa gaaacagaca
     5581 cctttgtttg attttataat aaccaataaa tcaataagta tagatgaaaa tattaaaaat
     5641 gtttcattct ctggtggagt agctgattat atttactatg atggagaaat aaaagattat
     5701 tttgaatatg gtgacttggg aattttactt gggcaagcaa taaaaaattg tgaatacctt
     5761 aaaaaagtaa atgtagttaa atctatggaa acaataagag ccacagtggt tggagcaggt
     5821 tctcatacta ctgaagtaag tggaagtaca ataacttaca ctagcgaaaa gtttccactt
     5881 aagaacttgc cagttttaaa attatcaatt gatgaagaaa caggtacttc aaaacaaatc
     5941 agtgaagcta taaaaaataa acttaattgg tttaaactag aaaatgattt acagaaagtt
     6001 gctatagcca tgataggaaa gaaaaatcca acctttttag atatacaaga gtatgctaaa
     6061 gggtttgtag atggaatgca acaaatcata gaaaatgatt taccttttat agtaattgtt
     6121 gaaaatgata tggcaaaagt gttaggtcaa gcaatatatg cattattaaa ttatgaaaaa
     6181 gaaattgttt gtatagacag tgtcaaggtt gaaaatggag actatatcga cattggaaaa
     6241 cctatagttg aaggtaaagt tttacctgtg gtaataaaaa cattagtttt taattaatag
     6301 aaggaggtaa gtaaatgatt ttaaatacaa aattatttgg acgtacttat cagtttaaag
     6361 gcttatatga tgtaatggct aaagctaatg aagaaaaatc aggagataaa ttagcaggtc
     6421 ttgcagcaga atcagctgaa gaaagaatag cagcaaaagt tgttttatca cacataaaat
     6481 tggaggatat aagaaataat cctgcagtgg catatgaaga agatgaagta acaagaataa
     6541 tacaagatgc tgtaaatgaa aaagtttatg aagaaataaa aaattggaca gtgtctgaac
     6601 ttagagaatg gctattggat tcaaatacta caagtcttga tataagaaga atatcaagag
     6661 gattaacttc tgaaatgata gcagcagttg caaaattaat gtcaaatatg gacttagttt
     6721 atgcagctaa aaagataaag gttactgctc actgtaatac aactatagga gaacaaggaa
     6781 cattatcagc aagacttcaa cctaatcatc caacagacga cccagatgga atacttgcat
     6841 cacttcttga aggtttaact tttggtatag gagatgcagt acttggatta aatccagttg
     6901 atgactctgt tgagagtgtt actaaagtat taaaaagatt tgaagaagta aaacaaaaat
     6961 atcaaatacc aactcaaaca tgtgtattgg cacacgttac tacacaaatg gaagcaataa
     7021 gaaatggtgc tcctgcagat ttaatattcc aatctatagc aggttctgaa aaaggaaatg
     7081 aagcatttgg atttaatggc gacactatag aagaagctag acagttagct ttaaaacacg
     7141 gtacagctac aggacctaac gttatgtatt ttgaaactgg acaaggttca gaattatctt
     7201 cagaagcaca tcatggaata gaccaagtta ctatggaagc tagatgctat ggatttgcta
     7261 aaaagtatga accattctta gtaaatacag ttgttgggtt tataggacct gagtacttat
     7321 atgatgctag agaagttgta agagcaggac ttgaagacca ctttatgggt aaactacatg
     7381 gtttaccaat gggtgttgat gtttgttaca ctaaccatat gaaggctgac caaaatgata
     7441 tggaaaactt agccatacta ttaacaacag caggatgtac gtattttatg ggaatacctc
     7501 atggagatga tgttatgctt aactatcaaa caacaggata ccatgaaaca gcatcactta
     7561 gagaaatgtt tggactaaca gcaataaaag aatttgatgc ttggatggaa aagatgggat
     7621 tctctaaaga tggtaagtta acagagttgg cgggagacgc atctgtatta ttaggatagg
     7681 aggtatttaa tatgaatgag aaggatttaa aagcattggt agaacaatta gttggacaaa
     7741 tggttggaga attagatact aatgttgtgt ctgagactgt taaaaaggca actgaagttg
     7801 tagtagacaa caatgcttgt atagatgata taacagaagt tgatataaga aagcaattac
     7861 tagtaaaaaa tcctaaggat gcagaagctt atttagatat gaaagcaaaa acacctgcaa
     7921 gattaggtat aggaagagct ggtactagat ataaaacaga aacagtttta agatttagag
     7981 cagaccatgc tgctgcacaa gatgctgtat tctcttatgt agatgaagaa tttataaaag
     8041 aaaataatat gtttgcagtg gaaactttat gtaaagataa agatgaatac ttaacaagac
     8101 cagatttggg aagaaaattt tctccagaaa ctataaataa tataaaatct aaatttggta
     8161 caaatcaaaa ggttttaata ttagttggag atgggcttag ctcagctgca atagaggcaa
     8221 atttaaaaga ctgtgtgcca gctataaaac agggtctaaa aatgtatggt atagattcaa
     8281 gtgaaatttt atttgttaaa cattgtagag ttggtgctat ggaccactta ggtgaagaat
     8341 taggttgtga agttatatgt atgttggttg gagaaagacc aggattagtt actgctgaat
     8401 caatgtcagc atatatagct tataagccat atataggaat ggcagaagca aaaagaacag
     8461 ttatatcaaa tattcacaaa ggtggaacaa ctgcagttga agcaggtgct catatagctg
     8521 aacttataaa aactatgtta gataaaaaag cttcaggaat agaccttaaa taaagtcgag
     8581 ggagtgtaga atatgaaaaa tgatgtaatt cgtcctaata tactaggaat taaaataatt
     8641 tcaaatataa gtccagaaat ggcacaaaaa ctagaattaa aatcacatca caaaagctta
     8701 ggtttaataa ctgcagactg tgatgatgtt acgtatacag cacttgacga agcaactaaa
     8761 gcttcagaag ttgatgttgt atatgcaaaa tcaatgtatg caggtgctgc aaatgcttca
     8821 acaaaacttg ctggtgaagt attaggaata atagcaggtc caagcccagc agaagtaaga
     8881 agtggtctaa atgcagtagt tgattttcta gagtatggag caacttttat aagtgcaaat
     8941 gatgatgatt caatagcata ctatgctcac tgcgtatcaa gaacaggtac atacctttca
     9001 gaggtagcag gaataagaga aggtgaagca ttagcttatt tagtagcacc accacttgaa
     9061 gcaatgtatg cactagatgc agcaatgaaa gctgcagatg ttaagatgtg tgaattattt
     9121 gcacctccaa ctgaaacaaa ctttggtgga gcattactta caggttctca atcggcttgt
     9181 aaagcagcat gtgatgcatt tgcagaagca gtaaaatcag ttgcagacaa tccaacagga
     9241 ttttaaggat agttagatgg atgctatagg cttaatagaa gtaataggat atgttgctgc
     9301 aatagaagct tctgatgctt gtttaaaatc tgcaaatgtt aatattgtaa gagttgataa
     9361 ggttggagca ggtattgtta ctttaacaat atgtggagat gtcggtgctg taaaagctgc
     9421 tttagaagca ggtgaaatag cagcttccag agttggtaca ttaagaacat cacatattat
     9481 accgagaatc cataatgaag taacaaatgc tttattcaaa actaaagaac ctaaagtctg
     9541 tgaagtttca gaagataaag aaaaaaactt tgagattaca ttggaaatag ctgataatgt
     9601 atcgaaaaat ttatcagaaa aagaaaatgt aattttagat gtcaatgaaa acagtggaga
     9661 tactgaaaat ttatatagtg atatagataa aacaaacaaa aaaattgaaa cagttgatga
     9721 tagtttaaca gaagctaatg aggaaaaaat agaaaacact aatgataaag aagtattgga
     9781 acaagttaaa atagaaaatt ccgattttga tagagatgac ctatcaaaga acagtgtaaa
     9841 aaaacttaaa gctatggcta aaaaattaga ttcttcaata acttataaag acttaaatgt
     9901 tcttaaaaaa gaagaattaa taaacttagt taaaaagctt agcagaaggg atagatagtt
     9961 atgacacttt tagataaaga tttagtatct atacaagaag taagaactct tgtaagaaga
    10021 tctaaagtgg ctcaagagaa actttctaaa atgagtcaag agcaaataga taaaatagta
    10081 aaagctgttg tagatgcagc atttaataat gctactaaat tagctaaaat ggcaaatgaa
    10141 gaaactggat ttggaaaatg ggaagataaa gtaattaaaa atacttttgc atctatggga
    10201 gtttatgaaa ctttaaagaa cacaaaaact attggaataa ttggtgaaga taaagaagcc
    10261 aatacgatag atatagctac accagttgga gttgttgcag gtcttatacc ttcaacaaac
    10321 ccaacatcaa cagtaatata caaaactata atatctctaa aagcaggaaa tactatagtt
    10381 ttctctccac atccaaatgc aaaaaactgc atacttgaaa cagttaatat aataagtgag
    10441 gctgctataa aggctggagc tccagaggat tcaatattat gtataactaa tccaagtata
    10501 gttggaacca atgaattgat gaaacataaa gatacaaact taatactagc aactggagga
    10561 gaagcaatgg ttagagctgc ttactcttca ggaacaccag ctattggagt tggaccagga
    10621 aatggaccag catttataga taaatcagca aatgttaaat tagcaatcaa aagaataata
    10681 gattctaaaa cttttgataa tggtgttata tgtgcatcag aacaatctat agtaatagaa
    10741 aaatctatgg aagaaactgt tttaaaagag cttaaaaatc aaggtgcata tgttttaaat
    10801 gaagaggaaa gtgaaagatt agcaaaattc atattaagag ctaatggtac tatgaatcca
    10861 caaatagttg gtaaaagtgt tgaggtaata gcaaatttag cagacttaac aaatgtacct
    10921 aaaaatacaa aagttctagt tgcaagagaa aataaagttg gtcacaatgt agcctattca
    10981 agagaaaaac ttacaccaat actagcgttc tatgttgaag aaaatgtaga agctattatg
    11041 aacagatgca gagaaatatt gttaaatgaa ggtgcaggtc atacattctc aatgcatgta
    11101 aatgatgaag aattggtaaa aagatttgcc cttaatatgc cagtatcaag aatacttgtg
    11161 aatactcctt cagcacttgg aggaataggt gcatcaacaa accttgtacc agcactaaca
    11221 cttggttgtg gtgcaatagg tggaagttca acttcaaaca atataggacc acttgattta
    11281 ataaatgtta aaagagtagc ttatggaatc aaagaaatag aagaacttgg aagagataaa
    11341 tctcaagttg aaaaatcaat gtttgactca atggataaag aagaattaat aaatcttctt
    11401 gttcaaaaac tagtcaaaga actagcataa ctaaaataaa caatgaaact tatggctatc
    11461 gctataaatt tctaaaaata taaactataa aaaactataa aattaaattt ttggaggata
    11521 agattatggc aagtgcaaac gcattaggaa tgatagaaac taaaggatta gtaggagcaa
    11581 tagaagcagc agatgcaatg gttaaagctg caaatgtaca attagtagga aaagagcaag
    11641 taggtggagg attagttact gtaatggtaa gaggagatgt tggagctgtt aaagctgcta
    11701 cagatgcagg agctgcagct gccgagagag ttggagaact aatatcagtt catgttatac
    11761 caagaccaca ctttgaagta gatgctatat taccaaaggt atcagctgag taatataaga
    11821 ataattataa aatttaaatt ttgattgaaa aagaggctat ctcaaaaatg agtcttacta
    11881 ataaactcaa tgtattttga ggtagtcttt tttatacatc taataggaga tgtgataaaa
    11941 tgggtgtttt aacagaaaac gaagtaaaaa gaaaaattgg aaaaaacaaa aataatataa
    12001 ctgaatttta tatagaaaaa ggtcaaataa taactccttc tgcaaaatct tatttagctg
    12061 agagaagtat tgagcttaaa tatgttgata aactttcaga tgtaaacaat gaacctaaag
    12121 aagttgtaaa agaagtagtt aaagagaaaa aatatagata tactactgta tttggcgcta
    12181 aattggatga aaaaccagaa catatgactc aattaaatgg aaatctttta gtttttaaag
    12241 accataaaag aataatattg agaggaaaaa tagattctct tgaatcagaa atactaaagg
    12301 tacagatact ttgtcagaaa ctagatatgc caaagcttgt taaggaattg caagaagtat
    12361 tagactttgt aagaaatata atgagatgtg aagttttaga tgaaaagcta ggggacttta
    12421 aacttttagg attaactcct caagaactta gagaacaatc tcactatcct aaaaaatact
    12481 ttggtatagg acatgaattc ccaagttatg aaatgggaga agtagtagta gctataaata
    12541 gtataagaag taatacacga gaggttgaac ttgcagccta tgaagcattt aagggtgagt
    12601 atggaaatgt tcacagagaa gatttaataa aggcacttaa tagattatcg tcagtatttt
    12661 ggataatgat atataaggtt agaactaata agtataagta gttggaggac ataatggaga
    12721 atctattaaa tgaaatagtg gaagaagtaa taactagagt taaaaaagaa gcatttatag
    12781 aagttgaagc atcaggaaga cacgttcact taagtagaga agatgtcgat aaattatttg
    12841 gagaaggata tactcttaca aaactaaaag acctatctca accaggtcaa tatgcatgta
    12901 aggaaagagt tacaataact ggtcctaaag gaagtataaa aaatgtagtt gtacttggac
    12961 catgtagaaa tgaaactcag gtagaaatat ctttaacaga tggttcaact cttggactaa
    13021 aagctcctat aaaacaaagt ggagacttag aaggtacttt aagtattaaa atatcaacac
    13081 aatatggtga agttgaactt gataaggggt taatggttgc taaaagacat atacacatga
    13141 ctcctaaaga tgctgaaaaa ttcaatgtat gtgataaaga gatagttgaa gctaaggtga
    13201 tgggtcaaag acctttaaca tttgatgatg tagtaataag agtgagtgac agttttaaaa
    13261 cttatatgca tatagactat gatgaagcca atgcatgtgg ttattcaaaa ggaactgttg
    13321 ctaaaataat aaagaaagca taaatttaac ctactatata aattacctct agggaggatt
    13381 taatatgaat tatgataacc ttgtagattt aataacggaa gaaatctata aaaaaataaa
    13441 tagtaatgaa ataaaaatat ctaataaacc taaagctgtg attgtatttg aacaagataa
    13501 caataaattt aacttattaa aagatgaatt tgaaatagta gaatttgata aaagtataag
    13561 ggaatgtgaa atagtgatag tttcaagact ttgtatgagg gggctatcga atatagcact
    13621 tggaaattca acaagtgatg aagaaagatt tatactaaaa atgattatga agggcaaaaa
    13681 agtatatgtc ttagatgaag gaattgaata caaaaaatat aaggatactg caccaaaagc
    13741 attatataag aaatttatgt catttgaaga tgaaatatgt aaatttggtg tagaaattat
    13801 aaaagactta aatgttataa ctaaaaataa gttaaatata aagaaagaac tagaaagtac
    13861 tagaggttct aaaaaagaag atacaaaagt tttagataaa gatgttttaa atttaaccag
    13921 tgtagaaagt gtaaaaatag ataatgaatt tagtttagat ttaaggaata aaaaacttat
    13981 atcggaagct gacttaagaa aaccaactat aaatggagtt aaaaacatat tagttaataa
    14041 aaaaagtata ataacaccat tagctgtaga ttttatgaga atacaccatc tcaaactaaa
    14101 gaaactgtaa ggaggtaatt ttatggatat aggaaaagtt gtaggtaatg tttgggctac
    14161 aagaaaagat gaaaaattat gtggccaaaa acttttagtt gtaaaaattt taaaaactaa
    14221 agacacatac aaggaaggct tcatggttgc agccgataat gcaggagcag gaaatggaga
    14281 cttagttctt atatccaaag gttcctcagc tagagagtcg attgaaaaat ctcatgttcc
    14341 agtagatgca actatagttg gagtagtcga ttcgtttgag gtgtttgatg aataaaagta
    14401 taggagccat agagtttaaa agcattgcta aaggtataga agtatccaat gaaatgataa
    14461 agaaatcttc agttgatgta ttgtacttaa aaagtatttg cccaggtaaa tttctaataa
    14521 tagtaggtgg agaaaattct tatataaatg aatgtgtaga ttatggaata aaacttggag
    14581 aaggatatat tgtagataac tttgtaataa atgcaatttc acaagaaata ttagatggat
    14641 ttaaaaataa gtatcaaaat ctagatagta tagtctctat aggcgttgtt gaaagtagta
    14701 aagtatgcgc tggaataaaa atgcttgata agactttaaa atctggcgac ttagtattag
    14761 taaaacttca attatctttt gctataggtg gcaaattagt atatatagta gcaggagatt
    14821 taagtagttt agaatatgca ttaaaagaaa gtgaaaatgt agtaagagaa aaagaagtaa
    14881 tatataagac tgtaatacca tcagtagaca gtcaaataat aaaaagtcta ataaaataat
    14941 ggggggaatt atgagtataa acgaaattat aatctatgta atggtagtct tcatggtact
    15001 tggagctata gataaatgta taggtaacaa atttggtctt ggagaacaat ttgaagaagg
    15061 tataatggca atggggtcac ttgcagttgc aatggttggt gttatatgtt tagcaccagt
    15121 tcttgctgat gtattaagac caattgtagt accagtattt aatatacttg gagctgaccc
    15181 agctatgttt gctggaagtt tattagctaa tgatatgggt ggtgcgccac ttgcacttga
    15241 acttgcgaaa gaccctaatg ctgggttatt tggtggatta atagtagggg ctatgatggg
    15301 acctacaata gtatttataa taccagttgc gcttggaata attgaaaaag aagaccaaaa
    15361 attcttagca acaggaatac ttgcaggtat aataacaata cctattggag cattcgttgg
    15421 tggattagtt gcaggattcg aggtaatgat ggttcttaga aacttagtgc caataataat
    15481 atttgcagta gttatagcac ttggattatt aaaattcgaa aatgctatga taaaaggatt
    15541 cacatacttt ggaaaaggtg tagtaatagt tataacttta ggacttgctg ctgccatagt
    15601 tgaagcttta acaggaatag ttgtaatacc tggaatgaaa ccaatcagtg aaggtataga
    15661 aatagttgga gatatagcaa tagttttagc aggagcattc ccattagtgt ttgttataac
    15721 aaaggtattt aataaaccat taatggcttt aggaaaagtt cttggaatga atgatgtatc
    15781 agcagcaggc cttgttgcaa gtttagctaa ctgtatacca atgtttggta tgatgaaaga
    15841 tatggacaat agaggaaaaa taataaatgt agcattctct gtatcagcag cattcgtatt
    15901 tggagaccac ttaggattta cggcaggatt taattctaat atgataacac caatgatagt
    15961 tgctaaatta gttggtggta taacagcagt attgttagct atggttatcg caaataagac
    16021 actaaagaag gagaatgtat aagatggata tatcaaatat agataaaaat ttaattgaaa
    16081 cattagtaag acaaataata gaagaaaaaa taagtggaac taaagacaca gtagattttg
    16141 ttagaaataa agacattagt ggaataactt ctataaagtt accaactgta aaagttagtg
    16201 aatctgatag attagataca gggaatccaa gtgatgtagt ttacacaaaa gatttattta
    16261 cattagagga aagcccaaga ttaggttgtg gtatgatgga aatgaaagaa acaacttttg
    16321 attggactct aaattatgat gaaatagatt atgtaataga tggaacttta gatataataa
    16381 tagatggaag aaaggtttct gcttcttcag gtgaattaat attcatacca aaaggaagta
    16441 agatacaatt ttctgtacct gattatgcta gatttatata tgtaacatat ccggcagatt
    16501 gggcatctca aaattaataa aaatcaagtt aagttaataa tcaaataaat aatttaaaat
    16561 acaaactctt aaataagcac gcatatttaa aaaagctatg aattaaaatt catagctttt
    16621 tttataaaga atgttatatt ttagacgtat tttttctata tttactggga gatatattat
    16681 attttttatt aaataatcta ataaaatgct ctacatttgg ataaccaata ctattttact
    16741 gataatcttt tccttatcac taatcttaca ttttaaaata ttatttttac tcgtaatatt
    16801 atcataagaa tttgtttctt atttaaaact aattagaatt ataaagttta ttgccatgtt
    16861 attaataaaa taaatccagt taaatcgagt gctagttaaa gtaataagaa acttttacaa
    16921 tggtttcctt tatcgattga aataaaagtt aatatggtat aatatctaaa gagagaatct
    16981 tatcaattag aatatgcata gaaacaagtg gcttaaaagg agacgttata tggaagagat
    17041 agtaaagaag gaacaagcaa gtatgaatga tatttttttg tatcttaaag ctaaatcaaa
    17101 ctcattatat aaatttgtaa tattatataa tgagtttatc aataaaccta aagattatgg
    17161 aactggggaa cttataaaca tggttgaagt acatatgcta acagcgattg aagaaaatcc
    17221 aggaataagt ccaactcagt tagctaaaat atggaacaga actaaaggtg caatatctca
    17281 aactatatct aaattggaga aaaagggtta tataactaaa gaaaagaaag aaggaaatgc
    17341 aaaaactata ttactatatc caaccacatc aggagtagag ttaagtaagg cacataaact
    17401 atatgacacc atagatgtta caagaacatt tgaatactta atcaaggagt gtacattaga
    17461 ggagatagat agtttttata aagtaattga tttgtttatt aatttattag aagaatctcc
    17521 gcaggaaaat aatgattctg aaaaagtgta gctgagattg aagttgaata aagagaaata
    17581 gatattatag aagtagtact taagtacaca ttatataaaa atataatagg tataggatta
    17641 aaaagtttag tggtttaaag tttaaccact aaactttttt aatgtctata atctactatt
    17701 atttaattta ctaaaaataa aaaaactcat tatattaaga tagaaataga aaaataacta
    17761 gaaaaatact ataatatatc agcttaatac agaaaaaagt taaaaaattt gagaaaaata
    17821 aaaatgaaaa cgttttatat tgacatagag aaaaagtagt gataccattt aaatatagtt
    17881 tagtagctaa actatattta aattataata actattttag ggggatgtta tgaatttaaa
    17941 acaattaagt gaaaaatacg aagaatatgt aattcaaatg agaagagaat ttcataagta
    18001 tccagaatta tcttttaaag aaataagaac aacccaaaga ataagggaag aattacaaaa
    18061 aataggggtc ccttttgtat ctattgaacc aaatatcgta atagctacta tacaaggcaa
    18121 aaatgaaggt aagacaattg caattagagg agatatagat gcgcttccaa tgcaagaaga
    18181 ttcagatgtt gaatacaaat cagaatatga tggaataatg cattcttgtg gacatgatgc
    18241 tcatggagct atgttactag gacttgcaca tatgctaaat gatataagaa aagatttaaa
    18301 aggtagagta ttattagttt ttcaagcagc tgaagaagtt ggtggtggac acaaggagat
    18361 aatacagtac tttaaggaaa atggaggacc ggatagatta atagctactc atgtttggtc
    18421 cgatattgaa gctggtaaaa tttctgttga accaggacct cgtatggctg gtgggtctgg
    18481 ttggagaata gacataacag gtagaggagg tcatggttct agaccagatc aaagcataga
    18541 tcctataaag ccagcttgtg atatagtttt aaaagtatct tctataccag ttaatcatat
    18601 aagtgtacta gaacaatgtg ttgtacatgc atgtgcaata catggaggaa ctacaatagg
    18661 aaatgttatt ccaaacaatg cagaggtaat aggtggatat agatatttta ctgaagaatc
    18721 aggcaaaaaa gtgtttgaca ttataaagca aatgagtaat ggtgttggac aagtttatgg
    18781 agtggatgtg aaggttactc atactggagg cataggacct gttatcaatg atgaagaagc
    18841 agttaagatg gctaaagaaa tagtttctaa tatagatgga ttggagttag attcttatga
    18901 gccaatatgt gcatcagatt gttatggtga aatattaaaa gagtattctg gcttttattg
    18961 ttatctaggg gtaggtaaca aagaaaaagg aataatatat gcacaacacc atcctaagta
    19021 caatatagat gaagatatat taaagttagg ttgcgaattt atggctaaat atgcagttga
    19081 atttttaaat aaaaataata actaaaataa atctaatttt ataaatgggg ggatgaaaat
    19141 gcaaggtgca ttaaaggaaa ataagaaaaa aagtggtgga ggatttttag gatggattga
    19201 aaaagttggg aataaaatgc cacatcctct agcattattt acatatatta cactgatagt
    19261 agtagcatta tctgcaatag caagcttttt aggtttttca gcaacaagtc ctgctacagg
    19321 aaagttaata aaagttatga acttaataag tgcaaaaggt ttagtactat ttatgcaaga
    19381 gtttgttaag aacttccaaa acttccctgt gcttggagta gttttagtta tggcagttgc
    19441 cacaggagtt tgtgaaaaga caggattttt ttcaactgcc ataaaaatga gtttatcaaa
    19501 agttaagggg aatgcggttg tatttataat agcttttata ggagtatttg ctaatcaagc
    19561 tggtgatgct gcttttgtat tagtaccaac tcttgcagga gcaatatttt atggattaaa
    19621 tagaaatcca ttagctggta tattctgtgg atttgcatca gttggtggtg gatttgcaac
    19681 agctgtaatt cctgggggat gggacgttac gctaacgcca ataactgtat cagctgctca
    19741 aactattcaa cctgcatttg atatgacatt attagctagt tattatgcac tatttgtatc
    19801 tgcgtttata gtagctacag tatcaacaat agtaacggtt aaatttatag aacctaaatt
    19861 aggtaagtat actggtaaac cagaaggtat agatgataat caaggttttg aggtcacaaa
    19921 agaacaagca aaagcagcaa agttagcagg ttttacagtt ttagcttatg tagcgttatt
    19981 aattgctttg tgtataccag ctaatagttt tttaagaagt ccagaaggtt ctttaatatt
    20041 tggagcacca cttatgtctt gcttacagtt ttttatagta atactattct tcttgccagg
    20101 gattgtgtat ggaatgaggg ttggtaaaat caagactgta aaagacttaa atgatatatt
    20161 atgtcaatca atggcagcaa tggcaccgtt tatcgtactt gcaatagtta taggtcaatt
    20221 cttaacgcta ttctcagtgt ctaatttagc acaggtacta gctataaaag gtggagcatt
    20281 actcaactca ttgccactac caccacaagc tataatactg ctattcttag tactagtagc
    20341 gtttataaat atatttgtta taagcggaag tacaaaatgg atgatatttg gaccagtatt
    20401 tgttccaatg ttaatgcaat taaacataca tccagcattt actcagtttg tatatagatt
    20461 aggagattct gtaaccaatc atttatcacc attaaatgca ttctttataa tacttttagc
    20521 aacagtccaa aaatacgata aaaatgcagg tatgggaact atattctcag ctatgatacc
    20581 ttacactata ggttactttg tagtacttgc agttttagta gtagtttgga tgacaatagg
    20641 tataccagta ggaataggtg gacaagtttg gttataaaac atatttaata attttgggag
    20701 gaaaaaaaat ggaaacaact gaaataagat acaattttga tgaagtaata gatagaagtg
    20761 gaacaaactg tcttaaatat gatcttaaga gtcaatttat gccagaatct ccagaagatg
    20821 cactaccact atgggtagca gatatggatt ttgcatgtgc ccctgagata attaatgcaa
    20881 tgcatgaaag aatagacaga aaaatatttg gatatagctc tcaacaaact gatgattatt
    20941 atgaagctgt atgtggatgg tttaagagaa gatttgattg gaaaatagaa aaagagaata
    21001 tattctttag cccaggggtt gtgcctgcgt taggattttt cacacaaata ttaacacaaa
    21061 agggtgatgg tatcataata gaaaagcctg tatactatcc ttttgaagat aaaataacta
    21121 acaatgaaag aaaagtagtt aatagtcctt taaagtttga agatggtcat tattctatgg
    21181 attatgatgg atttgaagaa ttagctaaag atgaaaataa taaggtgttt atattatgta
    21241 gtcctcataa tccagttggt agagtatgga aaaaagaaga gcttagaaaa atagtagaca
    21301 tatgtaagaa atatgatttg tggataatat ctgatgaaat acattgtgat attattcgta
    21361 aaggtgaaaa gcatatacca ttagaagtag catgtcctga ttataaagat agaataataa
    21421 cttgtacagc tccaagtaag agttttaact tagctggtat gcaattatca aatatagtaa
    21481 tcaataataa ggaattacaa gaaaaatgga tgcttgaaac agattctaaa ttagctatat
    21541 tcctacctaa tccattcgca atagttgcta ctaaagttgc ttataatgat tgtgaagatt
    21601 ggatgaatca agtaaatgag tatatagatg gtaatattaa atatgtagaa gattttgtta
    21661 atgagtatat gcctaaagta aagataataa aatctcaagg aacttactta gtttggttag
    21721 actttagagg gtatggatta gatgcaaaac agcttgaata tgtaatgctt aacaaggcga
    21781 aggtattgct agatgaagga tacatattcg gagaagaagg agcaggattt gaaagaataa
    21841 atgttgcttc acctagaaca attatagaag agtgtatgaa tagaataaaa tgtgcatttg
    21901 aaggattata atagtaatat ctaaataata tatttgtgta ttttaaaaaa agactatgct
    21961 aaaaaaatat ttcaaagcat agtcttttaa agttttatga tgagtttaaa aaatttcaaa
    22021 acgttactat tcacttatat gtttatatta gaaataattc cacaatatac tcattttcta
    22081 aagttg
//
LOCUS       DAEMGG010000059        22063 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEMGG010000059 DAEMGG010000000
VERSION     DAEMGG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.059"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            221..1033
                     /locus_tag="KPK24_002121"
     CDS             221..1033
                     /locus_tag="KPK24_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2299793.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            2081..2842
                     /locus_tag="KPK24_002122"
     CDS             2081..2842
                     /locus_tag="KPK24_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG2299794.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            2907..3689
                     /locus_tag="KPK24_002123"
     CDS             2907..3689
                     /locus_tag="KPK24_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG2299795.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            3956..4375
                     /locus_tag="KPK24_002124"
     CDS             3956..4375
                     /locus_tag="KPK24_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG2299796.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(4478..4744)
                     /locus_tag="KPK24_002125"
     CDS             complement(4478..4744)
                     /locus_tag="KPK24_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG2299797.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            4971..5672
                     /locus_tag="KPK24_002126"
     CDS             4971..5672
                     /locus_tag="KPK24_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299798.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            5754..6362
                     /locus_tag="KPK24_002127"
     CDS             5754..6362
                     /locus_tag="KPK24_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2299799.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            6686..7150
                     /locus_tag="KPK24_002128"
     CDS             6686..7150
                     /locus_tag="KPK24_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HBG2299800.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            7787..8803
                     /gene="gap"
                     /locus_tag="KPK24_002129"
     CDS             7787..8803
                     /gene="gap"
                     /locus_tag="KPK24_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG2299801.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            9198..10367
                     /locus_tag="KPK24_002130"
     CDS             9198..10367
                     /locus_tag="KPK24_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299802.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            10747..15000
                     /locus_tag="KPK24_002131"
     CDS             10747..15000
                     /locus_tag="KPK24_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG2299803.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            15149..15643
                     /locus_tag="KPK24_002132"
     CDS             15149..15643
                     /locus_tag="KPK24_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG2299804.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            15759..16136
                     /locus_tag="KPK24_002133"
     CDS             15759..16136
                     /locus_tag="KPK24_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG2299805.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            16342..16782
                     /locus_tag="KPK24_002134"
     CDS             16342..16782
                     /locus_tag="KPK24_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG2299806.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
     gene            17441..18748
                     /locus_tag="KPK24_002135"
     CDS             17441..18748
                     /locus_tag="KPK24_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299807.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYYNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    18834..19096
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            19222..20025
                     /locus_tag="KPK24_002136"
     CDS             19222..20025
                     /locus_tag="KPK24_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2299808.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            20063..20674
                     /locus_tag="KPK24_002137"
     CDS             20063..20674
                     /locus_tag="KPK24_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG2299809.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            20706..21377
                     /locus_tag="KPK24_002138"
     CDS             20706..21377
                     /locus_tag="KPK24_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG2299810.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            21675..22037
                     /locus_tag="KPK24_002139"
     CDS             21675..22037
                     /locus_tag="KPK24_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG2299811.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         8974 a         2090 c         3847 g         7152 t
ORIGIN      
        1 tcgttgatat aagtattgct ggaaaagagt gaataaaatc aaatttaata agcaaacaaa
       61 gtaaagctat agttttattg ttgtgcttaa aaatatttgg ttctatttta ctcttattta
      121 aactcatctt tttgaagtta aaccacagag taaactctgt ggttttttta ttttaaaaat
      181 aaattttaag gagtggtatg ttttgttaaa caaattatgt atgtatttag aaagaccaga
      241 gctttataaa caaagtgaaa ttaatttttg ggatgatgag tatatttcaa agcaattatt
      301 aaaagcacac ttagacacta attttgaagg agcaagtaga aattttaact ttatcgagga
      361 ttctgtaaat tggatagtta cagttgcaaa tccagctaat tatcctaaat tacttgattt
      421 aggttgtggt ccaggtttat atgcagagaa atttgcacaa aaaggttata aggtaactgg
      481 aattgatttc tcaaaacgtt ctattaacta tgcacaaaat agaaataagg agactaattt
      541 aaacataaat tacttgttcc aaagttattt aaatatgaac tataatgagg aatttgattt
      601 ggctacttta atttattgtg attatggagc tttgtcaact gaaaatagaa ggttattaat
      661 ggaaaaaatt tatgatagtt taaaacctgg aggaaaatta atattagatg tatttacaat
      721 aaataaatac aataattttg aggaaattaa atattgggaa ataaatgaag atggaggatt
      781 ttggagtaat gaaaaatata tgtgtctaca agataattgt aaatacaatg attataatac
      841 attagaacaa actcttgtaa taaccgaaaa agatgaaaat gtattttatg tttggaatca
      901 ctatttttca aaagaaagtt tcttattaga agttgaaaat attggattta agtctgttga
      961 gttttttagt aatgttaagg gtgaagcata ttctgacgat agtatgacta tgggattagt
     1021 tttacaaaaa taataatttt tttaaaatga ataaataaaa acttgaaacc tttaacaata
     1081 aaataacata ataaaagctt acatattgaa aaatggaaac ttttatgtta tttttagtac
     1141 attacaaatt tgcttatata aaaatcaata cttgaattat tatttttatg tgaaatttat
     1201 ctatagtata tttttataaa caagtaatgt tattaaaatt aagtatgtag ttaaaagtgt
     1261 atagcaaaaa ggctgttata ttaatgttag aggttcaggg aaaatatata aagcaaagta
     1321 tttacagata atgtagattg cacagctata agttagataa tagaattatg taatcaagaa
     1381 tttacaacaa agtataagat aaggataatg ctaaattgcc atacaggtgc tagatgtgta
     1441 aatggattta attaatacta ttgataaagt gcaacaagca cataaaccaa ttgatgagat
     1501 tattaaaact ataagagata tagttgacat aattaaaata ttggaacttg tttataattt
     1561 taaatcaaat caataatgat taagattaaa ataatattaa ggcatcaatg ttataagagt
     1621 acaatataag gagtactata tagtactcct tacttataca tattaggcaa taggaaattt
     1681 tttcatagtt aaattttcaa gtatttttta ggaaacttta taattgagaa ttaagggtat
     1741 agtatgtgat gaacttactg aatttataat taggttatat aatggaataa tcatcaatag
     1801 ttttacttta gggaaaatat atgcttaagg aagtatagct tactaatatt attaaaatgt
     1861 atatttaaaa tgataaaaaa agaattagac aagtatttag taaattgact atgcaaaatt
     1921 ctatcaagca ggaaccctat gactttagtc atgggaggtt cagtaggtaa cttgttgaat
     1981 taagtttata ttatttaaat aaactactta atttagataa tataaaaagt cagaatttta
     2041 agaatagagc tatataatct tagctattag gagaattact atgataggaa tatattttag
     2101 tggaacagga aattcaagat actgtgtaga aaaattttta caagaatatg atataacagc
     2161 aaattcttat tctattgaag ataatgaact tttacagcac atcaataatc atgagaatat
     2221 tatttttagt tatccggtgc aatatagtaa tgtgccaaaa atattgaaag attttataat
     2281 aaatcattca actttgtgga atggtaaacg tgtttttatt attgcaacta tggctctatt
     2341 tagtggagat ggctctggtg tgttatctag aatattaaaa aaatacggtg ctataactgt
     2401 tggtggatta catgtgaaga tgccagatag tataggagat gaaaaggtat taaaacattc
     2461 tttagagtat aatagaaagc ttgtaataaa agcagaaaag aaagtaacta aagctgctaa
     2521 aaaactgaaa catgggtcac caccaaaaga agggttaggt tttttgagtc atattgtagg
     2581 gttatttgga caacgattgt attttatcaa caaaacaaat atgtatacag ataaactaaa
     2641 aattaatact gaggaatgta ttgtatgtgg taaatgtgtt aatttatgtc caatgaagaa
     2701 tctagtaatt aaaaattgta tgattgttgc aaatgggcaa tgtacaatgt gttatcgttg
     2761 tgtaaataat tgtgctaaaa aagcaattac attgttaggt aaaaaagttg ttgaacagag
     2821 taatattaaa aaatatatgt aagaaattta aagttttatt gaatgtaaat tgatgtatat
     2881 tttatgttag aaagagaggt taaataatgg aatactacat agttgattca tttgcaacta
     2941 aactatttaa aggaaatcct gctggagttt gtgttcttga cagaagaata cctttagagt
     3001 taatgcagaa aatagcagaa gaaaacaatt taccagaaac agcatttgta gttaaaggta
     3061 aaggaaatta tgaattacga tggtttactc ctaaagcaga aattgattta tgtggtcatg
     3121 caactttagc agcagcttat gtaatatcaa attttattga tgttaatgta aaaaggatag
     3181 atttctttac tcaaagtgga aagcttgaag ttacacgaaa tggaaatcta tatgaaatga
     3241 tatttccaga aataatgcct attgaaatag agttatcacc acaacaagct aatttgatag
     3301 gctgtgtacc aagtgctgtt tattcttcaa gagatttaat cttattgcta aacagtgaac
     3361 aagaagttat aaattataaa cctaattacg cacaattgcg taaattgaca gactggttag
     3421 gtataataat cacagcacaa ggaagtaata cagattttgt atcaagatat ttttgtccag
     3481 aattagattc agaagaccct gttactggtt catcacattg taatcttatt ccatactggt
     3541 ctgaaaagtt gggaaaacac aaaatggtag ctgctcaact ttctaatcgt ggtggtataa
     3601 ttcagtgtga agtgttgaag gataatactg tgaaaatttc tggtgaagca gtcttgttta
     3661 tgcagggaac aataaagatt gatatttaaa taagtataat aactaattta ttatgaaaaa
     3721 taattaattt tcttagtgtt aacaataata aagtagagtt aaaaaacttg atttcactta
     3781 aatcaatatg atagatgttg atactaagat tgacaagcat gattaaaaaa gataatattt
     3841 aatctataaa gataaaaaca cagtctggtt ggtagtccag acgcagcaat agctgtcagt
     3901 aacctgcctc cttggttgtc cgttctttag aaagtaattt aaggaggaac tagcaatgga
     3961 caaaattagt ggaaaattaa cagtcttatt tgaagaacca ttttgggtag gtatatttga
     4021 aagacaagat ggtaaaaaat atgaagcatg tagggtagtt tttggagcag aacctaaaga
     4081 agtagaagtg tatgaattta tacttgaaag atttttctca ttagattttg gtagcattaa
     4141 attagaaaaa aatgtgacca aagacaacat tggatacaaa agaatgcaga gaaaagtaaa
     4201 aaaggaacaa gaaaaagaaa ctatagggac taaggctcaa aatgctctta agcttcaata
     4261 tgaagaaaga aaacaggata gaaaaaacct agctaaaaat agaaaagaag aagaaaaaga
     4321 aagactattt aatttgaaac aagaaaagag aaaagctaaa cataagggtc attaatttta
     4381 attaaatatc tttttaataa ttgctagaaa aaggtgtata aaatttttaa ttttatacac
     4441 cttaatttat tgaaaagaat aaggttttat atttcaatta ctttaaaata taaaaatata
     4501 ctaataaaaa ttgtaaaaat agtataaatg gtataccaaa tgtaaattta atgtgtttag
     4561 ttttatgtct aaaagaatac attccaatta tagaacctat actacctcca attactgcta
     4621 tagacataag agtagcttct tttattctcc attcgtttct aattgctctt ttcttatcta
     4681 tgtacattga aaagaaagca atagaattaa tcactataaa atatattaga ataaaatttt
     4741 tcacagtctt gcctccataa aaatatatta tacattataa aacaagatat ttgttttgga
     4801 aagttaaatt tgtaattagt acatataaaa tatttgataa gaacaaatat aatattaatt
     4861 ggaaaattta aaaaaattaa acaattagca aatagggggt atatttataa gacacaaagt
     4921 gataaaatct atttagaaaa taatgtaaaa taacaggagg ataaagcaaa atgaaattta
     4981 tagtagaaaa agaagttttt gataaattag aaaatgtatg ctttggtgta gtagttgcaa
     5041 aaggaataga taatactaaa gaaatagaaa gaataagcaa ccttttagat ataagtatag
     5101 atagagtaga agattacttc aaagataaaa aagtaaagga atcagaagaa attataccat
     5161 atagagaggc atttagaagt ctaggcatga acccaaataa atttatgagt tcgatagaag
     5221 caatgacaac aagggttgct aagaataaga aacttcctca tataaatcct attgtagatt
     5281 taggaaactc tatttcttta aaatatttac ttccaatggg tgctcatgat atggatttta
     5341 gaaatgatga tgtatatgtt agattttcta aaaaagggga taaatttgta ccatttggag
     5401 aaactgatgt tgaattaatg gaagaaggag aattaattta ttcagttgga gacatggtaa
     5461 aaactagaag atggatatgg agacaaggag aagaaggaaa aattactaat agctcaaaaa
     5521 atatattttt tcctatagat ggttttactg atgctaattt agataaggta atgagtgcaa
     5581 gagaagaatt agcaaaattg ttaaaggaaa tatttaattg tgaaattaaa gtaggttttg
     5641 tagataaaga taatcctgaa atggaaatat aataagttgt tgaatacttg ttaagatatt
     5701 atatttactt aatgatgtta aaaaggtatt aaaaaagaaa ggtcgtagta aatttgaaaa
     5761 aaactataat aaagaatgta aatccaatga ttgaaaataa gtttatagga ctttttgaaa
     5821 tagagtataa aaacaaatta gatgaagata aggtttggat ggtagcatca agaaaaagta
     5881 gtgagcaatt aaaaaatatt tatttgaaga atgaagaaga tagtgtagat gcagttgtaa
     5941 tagttgggct tcataaaagt agtaaaaaat taattttaat aagacaattt agagtaccta
     6001 ttaatggata tatatacgag ttaccagcag gtcttgttga tgaaggagag tctatagata
     6061 tttccgttga aagagaactt agagaagaaa caggacttac tcttttagaa attaaaaaaa
     6121 ataaaagtat tgataaagtt tatttatctc ctggaatgag tgatgaatct gttgcctttg
     6181 tttattgtat atgtgatggt gatataacag atgaattttt agaaccagat gaagagattg
     6241 aagcgctttt aatttcgcaa gaagatgcaa aagagatttt acaaagtaat cataagatag
     6301 acacaaaagc atttttgatt ttacaaatgt ttgtgagttt aggagctaaa ttatttgaat
     6361 agatatatag aaaagaggtt atatagaaga gtaaaataat aaagtttatt ttacttgtgt
     6421 aaattataat ctcttttttt atacaataaa gtataatttt gtgtattttt aaataagtga
     6481 tttgactctt aaattttcat aatatgttat aaaccaattt aagatactac aataattaaa
     6541 attccaaaat tatttaacat atttgcaatt aaaatgagaa aatgtaaagg ctgaatgatt
     6601 gaaaaatttg atgtaaaata gtagcttagt ttaatactat agtttgtagg gttaaaaatt
     6661 agaaattgta agggagataa attctatgaa aaaattagtt attattttaa ttagtatcat
     6721 tagtatggtt agcttgacag cttgtaaaaa aagcaatgaa agtaaatata ataatttaaa
     6781 caacaaagaa tccggacaaa caaccattga aatggactta gataaaaatt atgatacttc
     6841 agacccattt gttaatgcac gtttgttttg tgtgtctaat gatatagatg ttttagacac
     6901 agagatttct tttaaaatgg atggagatag tggtattgtg gaaataaagg acaataaaac
     6961 agatgaaact ttatggagta atacatggca tggaagcgtt gataatgata cattcacaat
     7021 ttctctttct aatatacaaa aagagaaaga gtatgctata tgctttactg gaattaaaat
     7081 aaatcatgca gttgtaaagg ttacttttga aagcaattta gttaaggaga aagaaaaacc
     7141 ctcaaaataa attccagctt ttctataact tgatattcag tacataaaag cagttgatat
     7201 ttatgattga ctgctttttc ttatcaaaaa ggataggttg tgcattttta cttgaaatag
     7261 tttatgcaca tcaaaagttg gtgagcatga tattggaaat ggtaagcatg aaatcttgat
     7321 aatacaagga aataaattta agttttcaat taagtatatt tccataattt agttaacaat
     7381 aagctaatta ttatttatat tttgatatat aaataataat tgtagcaaat tttaaaaaat
     7441 catatatttt ttgaactaaa taaaaattta tttttattat tttaaattag aggagattct
     7501 tgaagatata ttatttttaa aaataagtat agtttaaaaa ttcaaaaaaa agatggcaaa
     7561 atatgtccca agggacattg agtaaaataa atgatttcag cttatttaca ctaaaaagta
     7621 catactaaat gaggggtaaa ataaagaaaa atgatatact aattgtcttt tgataataaa
     7681 aaaacatgga atttgattat ttaatcaaga aaaagtattg aacaaatgat ttatttagtg
     7741 tacaataatc atgtaaaaac atagtaatgg agggagacat ttcataatga aaattaaggt
     7801 aggaatgaac ggatttggaa gaataggaag agctgtatta agaatagctc aagaagaatt
     7861 aggagataat atagaaattg tagccataaa tgcaagagca acaacagaat ctttggcaca
     7921 tttatttaca tatgattcat gctatggaac ttttagaggt gaagtagagg caaaggatga
     7981 agacactcta gtagtaaact ctaaagagat aaaaatatta agatataatg acccagaaga
     8041 attaccttgg aaagaattgg gggttgatat agtaatagaa tcaacaggat tatttacaca
     8101 aagagaaaag gcagaaaaac atattaaagc aggagcaaaa aaagtaatta taacagctcc
     8161 aggaaaaaat gaagatataa ctatagttat cggagttaat gagaaagaat atgataatga
     8221 aaagcacaat ataatttcaa atgcatcttg tactacaaat tgtctagctc cttttgctaa
     8281 agtattggac gaaaaatttg gtatagtaaa agggttaatg actacagtac attcatatac
     8341 taacgatcaa agaatattag ataaaagcca taaagatttg agaagagcaa gagcagcggc
     8401 tgaatctata atacctacca ctacaggagc agcaaaagct gtgtcaagag tactaccaca
     8461 attagaagga aaattaaatg gtttttcact tagagtacca acaccaacag tttcacttgt
     8521 tgatttagtg tgcgaactta aagaaaacgt tacatctgag caagttaact ctgtgttaaa
     8581 agatgctgct gaaggagaat taaaaggtgt tttaggatat tgtgacaagc cacttgtttc
     8641 tatagattat aaaggagatt caagatcatc tattatagac gctctatcca caatggttat
     8701 agaagacaat atggttaagg tagtttcttg gtatgataat gaatggggat attcttcaag
     8761 aactgttgac ttagtaaaat atgtagctga aaaattaaaa taatatatat ttcttaaagc
     8821 aaattttaaa aaaatttttt aaaaaaatta tataaaacta aaaaaaaaga ttatgaaaaa
     8881 tattgaagga aaaatttttt cataatcttt tttctttcat gcaaaaagat acaagttaca
     8941 acttatattt gcatattagg tcgttagaga agtatatata tatttagtaa ggaatcatac
     9001 tttttataaa atgaaagata agatgtcgaa aacttgcata aatctgaaaa atttttatta
     9061 ttaaaatact gatttccatt ttaaaaatga taaaaaaatg acaaataaag ttgcaaatag
     9121 taatacttat aagtataatt aaaaaagaga aaaggttttg ttaaacttta taacaaaaaa
     9181 agaagggatg attcaaaatg gagaagttaa cagacaggag acaggttaag gcagtaagca
     9241 tggaaacctt cattttccta atattactag tagttggttt tgggtatgtt ggtagtatta
     9301 tgggagcagg aatgatgttc aaagtaataa tgagtacagc acatgcttta ctattggaga
     9361 cagttttctt aattatggct atggcagttc ttgcaggcgc attaagcgca ctactttcag
     9421 agtttggagt aatagcgtta attaataaaa ttttcgctgt atttatgaag cctttatatg
     9481 gactacctgg tgctagtata gcaggagcta taacaactta tttatcagat aatccagcta
     9541 ttataccttt tgctaaagat aaaacattca cacagtattt taaacaatac caagttccag
     9601 cattatgtaa cttaggaaca gcctttggta tgggacttat cttaacaaca tttatgattt
     9661 ctcaaggtac agaatatgta cttccagcat taattggtaa tttaggtgca attataggta
     9721 gtgtcataag tgttagaata atgttgacat ttactaagaa gttttataaa tacaatccgg
     9781 aagaagataa agcaacagga acattagaga aaaaggatga atttagagaa atcagagaag
     9841 gaaatgtatt tcaaagagct ttagatgcaa ttcttgaagg tggtaaaatg ggggtagata
     9901 tgggaatggc tataattcct ggagtcctag tagtatgtac attggtgatg ttattaactt
     9961 ttggaccatc tacagatcct gttacaggtc aagaagttta tacaggagct gcatatgaag
    10021 gaataaaatt acttccagtt attggagata aacttggatt tatattagaa ccattatttg
    10081 gatttacatc accagaggca atagcattcc ctataacttc attgggagct gttggcgcag
    10141 caatgtctct agttccagag tttataaagt caggagctat aactcctaat gatatagcag
    10201 tatttacagc tatgggtatg tgttggagtg gatatttaag tacacatatt ggtatgatgg
    10261 atgcattaaa tgctagacag ttggcaggta aagctattct ttctcataca ataggtggtt
    10321 tatgtgcagg agcagctgct cactttatat ttacactcgt aggataaatg taatcagcta
    10381 agataaaaaa taatatacaa aaaatggttg ttgatatgta ataagaattt acatatcaac
    10441 aaccattttt ttctgactaa agataattta taatacataa acattaagtg gatattagag
    10501 ttaagttata tttatgtaaa aagtaacaaa aatctgtaga aatttatgac aaaatttagt
    10561 acactaacta tatgttctta tttaaaattt aaggttagaa ttaggaatta cctagtagag
    10621 taagctttga ttaataaaag aatcataaaa taaaaatatt agtcataaag attataaata
    10681 acttacttaa aataaagcca tagaacttac aaaaattaaa aaagcatata aatatggagg
    10741 ggtacaatgg atgaattatt tcatatagga attgatgtag gttctacaac ggtgaaggtt
    10801 gtggtactta ataatagtaa taatatagtt cataaagaat atagaagaca ttattctgat
    10861 gtaaaaaaat cagttaaaga agtactaaat ggtatatatg aaaaattggg agatataaat
    10921 actaccatta taataactgg ttcaggtgga attggaatat caaaaaaatt aggtgtaaaa
    10981 tttgttcagg aagttatatc tagtacaaaa tcaatagagt attttcatcc tgaaactgat
    11041 gttgtcatag aacttggggg tgaagatgca aagataacat atctaagtgg aggaatagac
    11101 caaagaatga atggaatatg tgcaggtggc actggagcat tcatagacca gatggcatca
    11161 cttttaaaga cagatgccag tggattaaat gaacttgcta aagggtataa tgttatatat
    11221 cccatagctt caagatgtgg tgtttttgct aaaacagata ttcaacctct tataaatgat
    11281 ggagccaatc agactgatat agcaatgagt attttcaatg cagttgtcgt acaaactgtg
    11341 agtgtccttt cttgtggaag aaaaatagaa ggaaatgttg cgtttttggg tggtccattg
    11401 tactttttat ctgaacttag agaagcattt aaaagagtat tagatttaaa agatgaaaac
    11461 ataatcttcc cagaaaatgc acaactttat attgctattg gagcaggatt attatcagta
    11521 gatgaagata atatacaact aaaatctcta atagaaaaat tggatagtat aaagaatatt
    11581 gaagatggag aagtaaattt attagaacct ttatttaaag ataaaaatga gtatgaagaa
    11641 ttttcaagaa gacatgaaaa agaaaaaata aattatgtgg acataaattc aataaaaagc
    11701 aattgttatt taggaattga tgcaggctca actacaacta aagcagcact tattgatgag
    11761 gatggaagac ttgtatattc ttattataat agcaatgaag gaaatccatt aaaaacaacc
    11821 attaaagtta taaatgaagt atatgatata ttaccaaaaa atattaaaat attgagttca
    11881 actgtaactg gatatggaga aggtcttatt aaaaaagctc ttaaaatcga taatggagag
    11941 atagagacta tagcacatta caaagctgct aaatttttca acaaagatgt agattttata
    12001 cttgatattg gtggtcaaga tatgaagtgc ctaaaaataa aagatggagt aattgataat
    12061 ataatattaa atgaggcttg ttcatcaggt tgtggctcat ttttagagac atttgcaagt
    12121 tctttatcta tgacaataga agaatttgcg catgaaggta tttattctca aaatcctgta
    12181 gatttaggtt caagatgtac tgtatttatg aattctagag taaaacaagc tcaaaaagaa
    12241 ggtgcaagtg ttggagatat atcagcaggg ctatcttatt cagtaataaa aaatgcttta
    12301 tttaaagtaa taaagattag agatttaaat gagattggta ataatattgt tgtacaaggt
    12361 ggaacctttt ataatgattt ggtgcttaga agctttgaaa aactaattgg aaaaaatgta
    12421 attagaccta atatttcagg aattatggga gcttttggtt cagcacttat agctaaagaa
    12481 aaatatgaaa gtggatacaa aacatccttg ttatctaagg aagagttgaa taatatcaag
    12541 ctagaagcaa gtgtaactag atgtaaaggt tgttcaaatc attgtcttct tacaataaat
    12601 aaattttctg ataatgaaat ttttatttca ggtaatagat gtgagaaagg tgaagctatt
    12661 tatggtgata aaaaaatagg attagaaaag aaaaaaccaa taaatttatt taagtacaaa
    12721 tacaatagaa tttttagata caaaccattg gaaaaagaag aagcaaaaaa tggggaaata
    12781 gggataccaa gagtattaaa tatgtatgaa gattatccat tttggtttac ttttttcaat
    12841 gctatgggct ttagagttgt actttcagat aggtcatcaa aacagttata tgaaactggt
    12901 ataactagta ttgcatctga aacagtttgt tatccaggaa aattagttca tggacatata
    12961 gaaaatttaa ttgcaaaagg tataaaaaat atattttatc caagtgtaac aaatgaaaat
    13021 aaagaggatt caaatgcaga taattattat aattgtcctg ttgtaatatc atattctgaa
    13081 gtaataaaaa acaatgtaga gaatataaga aataagaaca taaattatat aaatccattt
    13141 ataagtctaa atgacaagga aaaattgaag aaaagactct atgatgaatt atcaagacat
    13201 ttttctgata taaaaataac taaagaagaa gttaatcatg cagttgataa agctactgat
    13261 gaacagaatt tatttaaaat ggagatacaa gcagcaggtg aaagagcact taaagaaata
    13321 aaagaaagaa atatgaaggg gattgtgtta tgtggaagac catatcatat agacccagaa
    13381 ataaatcatg gaatgccaga gcttataaat tcattagata tggcagtgct tactgaagac
    13441 tctatatgtc atttagccaa tgtacaaaga ccacttagag ttgtagacca atgggtatat
    13501 cattcaagat tgtataaagc agctagtttt gtaagagata aaaactattt ggaacttgtt
    13561 caacttaatt catttggctg tggattagat gcagtaacta cagaccaggt tcaagaaata
    13621 cttaatgaaa aatcgaagat atatactatt ataaagattg acgaaggcaa caatttagga
    13681 gcagctaaga taaggatgag gtccttaaaa gcagctatgt ttgagagaga aagcaaaaat
    13741 atagatgtaa aaaatataaa ggtaaacagg atacagtatg ctaaaaataa tcagataacg
    13801 aacaaacata ctatattagc accacaaatg tctccaatac atttccaatt ccttgaaaaa
    13861 gctgttaact taagtggtta taatatagaa attctaaaag atactgatag tagtgtaatt
    13921 gaagaagggt taagatatgt aaataatgat gcatgttatc cagctataat agttgtaggt
    13981 cagctaataa cagcattaaa atctggcaaa tatgatttaa ataacacttc tgttactata
    14041 actcagactg gtggaggttg tagagctaca aattatattg gattcttaag aaaggctata
    14101 tacgatgctg gatttaaaga tattcctgtc attgcactta gtgtaaatgg aatagaagat
    14161 agtggtataa tggataatat atcactaaaa ttaatcaata gactctttat gtctgttgtt
    14221 tatggtgatt tgctgatgaa agtactatat agagtaagac catatgaaaa agttactgga
    14281 agtgcaaatg ctctatatga aaaatgggtt gatatatgca aatcttctct tgttaaagca
    14341 aaaatttctg tatttactag aaatataaaa gatatagtaa aagaatttga taatttagag
    14401 atactagata tcaaaaaacc aaaagtaggt ttggttggag aaatattggt gaaattccat
    14461 ccaattgcaa ataataattt agtagatata ttagagcgag aaggtgctga agcagtcgtg
    14521 ccagacctta caaatttctt cttaagttgt gcctttaata ctatatacaa acacactcat
    14581 ttagaaggaa gtagaaagag tagaatgata ggagaagcat ttatatacat aactggaata
    14641 tatcaaagag tatataaaaa agcacttgat aagagtgaga gattttatgc tccagccaat
    14701 ataaaaaatg ttgctaaaag tacagaacca gtagtgtcat taggtaatca aactggagaa
    14761 ggttggcttt taacaggtga gatggttgaa ttattaaatg aaggtgtaga aaacattatt
    14821 tgtatgcaac cttttggatg tttacctaat cacataattg gtaagggttc tataaaagaa
    14881 ttaaagaggt tgtataaaaa tgcaaatata atacctattg attatgaccc ttctgcaagt
    14941 gaggtaaatc aacttaacag gataaaactt atgctttcta aggcttttaa aaatatataa
    15001 gatttatatt ttgatatgtt aaaagttaat gaatttaaaa atatactcta tattaagtat
    15061 ctataaaaaa gagttatcct attttttagg gtaactcttt attagttaaa aaattatatg
    15121 tatactgata ttaaaggggg gaagtaatat gagtttagta tatgatgttt ataaatcaaa
    15181 attaggaaat ttgtacatat taagtgaaga tggttttata gttagtatat acataggaga
    15241 tgaaaaattt aataatttaa aaaatgaatg tattaaaaaa ggaattgata taaagagaag
    15301 taaggaagat ttaaaggaag caataagcca gatagatgat tactttaatg gaaaaagaaa
    15361 agtctttaat ttaaatattc gtattgaagg gactgaattt caaaaatcag tttataggga
    15421 aatgttaaaa ataccttatg gagaaacatt gtgttattca gatatagcaa gaaatataaa
    15481 caatccaaaa tcagtaagag caataggaca agcaagtaaa agtaataaaa ttccaataat
    15541 aataccttgt catagagtag ttggcaaaaa agatattggt ggatatatgg ggaatcattc
    15601 tgatttgaaa gagatactat taaatttaga aagacagagt taacttagaa agaaatgttt
    15661 ttattgttaa tatttgatat aataatagta aaaagaagta ttttttacaa aaacaatgaa
    15721 gaatttattt taatttataa aaatgagggg gaattttaat gtgtgataaa gttatagcat
    15781 caaaatatca tgagaaggga tttaattgtg cagaatcagt tataaaagca tataatgagg
    15841 aatttaatac agatatacca gtttgtttag gaagtggact gggttcaggg tgtggggttg
    15901 ctagtttatg tggtgcagta aatgcatcaa atataataat tggatatgta aaaggcagaa
    15961 accacgaaga tgaaagtact aaagctaaag tatatgctaa agatttaact acaacagtaa
    16021 gaaaagagta tggttcagag ttatgtatag acctaaaaaa agatttagtt gcttgtagag
    16081 aaataatgga tttcgcatat gatagtctaa aggaaacttt aaaaaaagaa ttataagaaa
    16141 ataagctatc aagtataaat tatttgatag cttattttta tagtcgatac taaataaatc
    16201 aaatatattt tagaattatt atttgtaaat aaacagcaaa taattttagg atttttattt
    16261 aatatagttt ttatttatat ttatttttga atatgttata ctttgtttaa ataaatattt
    16321 gtaatgaggg agatgagaag attgaagaat ctcaatttac ttattaataa attatattca
    16381 aaaaatcata atgaagcata taaaactttt ttattcttag aaaatgaaag tttaaaatca
    16441 aatattacat attgtttttt tgactcattc ctagaaatga ttgataacga aaactcatat
    16501 attcgaacaa ggggattgtt attaatctct gcaaatgctc agtgggatat tgataataaa
    16561 attgaaataa atatagatag tatactaagt catatcgtag ataaaaaacc ttctgtatca
    16621 agaatgttta ttaaatccat acctaatatt acaaagtata aagaaaatct tattaatcgc
    16681 ataaaaatgg aattaagtaa tgcagatata agtatttata ataataatat gaaaccatta
    16741 gtagaaaagg atataaacga tacattatct aatattagtt aaatattaat atagaaattg
    16801 aatattatct agttacaatt atttataact aagataataa aacaacaatg gtgctaattt
    16861 atatgtataa attaattatg aaaaatttat ataagtaaaa tatattaaca taaaggtaaa
    16921 attttgtata ctaaaaggga atatttacag tttaatggca gttttaaaat aataaattaa
    16981 agctgtcatt tttatttaaa aatttttaga aaaagtacaa tagtgttaat gtgtgaatta
    17041 gataaggtgt atataatagt ttagatgata tgatataaaa tatgttgtaa attaaatgtt
    17101 ttctttgaca atatatacac aaggatgata atatctatat gaataaaaga tagtgtgtaa
    17161 tttgataata tgtaattata acattatgat ataattacaa taaagtatgt aataatctgt
    17221 agaaatttca agctagttat ctaagttttc ggataactaa atctaaacgg aatacataat
    17281 gtattaaata aatgatgata ctaaaatgat actattttta tagaaattta ttgaggtata
    17341 taattaatga aaataatata ttaaattata taaattaaat tatataaatt aaattatata
    17401 atatattgag ttttacataa aagcaaaggt gggaataaaa atgaaagaaa ttgctgatgc
    17461 tataacatca gagtatttaa gctatggtat caatgaagat attagtatct atgaaggtag
    17521 attttgcata tatttagata aaaaatatag atgtaatggg aaaatttatt ataaaatgac
    17581 acctccaata tctataagtt ttaaagcaga cataggttgt gttgaagaga tagacaatga
    17641 agatgacaat ttagctttag attatgataa tgctattctt gaagttcatg gatataaaat
    17701 tatttctata acaataaata ctttaagtga atttagtgta gaaggttata tcaatgatga
    17761 ctgcataaaa tctaagaact cctatgttga atatgtagat tttaatataa taaacttaga
    17821 taaaatacct ggaaaactca ttaaatacaa tgataaggta tacgcaggta gaattgaatt
    17881 tgatataaat gattatgttg taactataga caagcgatat gattatagaa aagaattaaa
    17941 atcagaatta aagtctaaat ctggagcaat aataactcat ataggtagga ttagaagaaa
    18001 agatggtaga atttttagaa caaataatac aataaatctt ttagatagga tatcaacagc
    18061 tttgagtttt atgtgtggaa gatatgtagg gttttgtttg gccaaagggt atagaagtgg
    18121 taatgaagtt tatagaatat ggaatgaaaa tcaaatatct ccttttagat atgtaccaac
    18181 atggtcagat acactttcaa attattataa tatggaaaaa tatataagcc ttatgtgtaa
    18241 aaagctagaa gatttttatt atggttctgc tataaagagt gttgttgatt ggtacataga
    18301 atctcttgga agtgctacca tggaaaacaa tataatatct gtacaaattg cacttgaaac
    18361 tttgtcatat gtaattctag tggaacaaaa taagatactt acagatgaag tatttgactg
    18421 taatttggca tctaaaaata taagactctt acttgataca tgtaagatac catatggtaa
    18481 acatgaatta aatatatttg ataatataat aaaaaataaa tttgatgatg gagtggattt
    18541 agttatttat ttaagaaata gcatagttca tccaagtaga aagactcata gagctgtgtt
    18601 agaggttgaa gatatttgga atataatttc aataggaacg agatatattg aacttgtttt
    18661 actctttatt ttaggttata gaggagaata ttcaaacaga ttagtagaaa gatgttatgg
    18721 tgaagtggaa gttgtacctt ggaactagat ttttatattt attttaaaat atatattgac
    18781 atatattgcc ttatgctata aaatcaatat cataataata attcagaata acattaaatg
    18841 ctaagaaaag aaatagtaaa agtagttaat cttaaacaga gagtttccga ttggtgagag
    18901 gagcaataga ttttactttg aacacatctt tgagcaggac tctgaaagag tagaaatact
    18961 ttattaggag atttccggaa tccttgcacc cgttatagtg ctagagtata ataattttat
    19021 tgtacttaaa gaggttagtg tcgtgaggca ttaatgaaca gaggtggaac cacgagagtt
    19081 aactcccgtc ctctagcaga agctagaggc gggagttttt taatataatt ttttaagcgc
    19141 gcagagaatt atattgcaat agatacactt tttaaaaaat aatcaaaata ttttaaaaat
    19201 taaaaggcga gggagaatat tatgaaaaat atattaaaaa aagtaggaat atttacaatt
    19261 atgttaggtt tattaggtgg agttgtaggt tgttcaaaac cagataatga aaaagataag
    19321 gatgcttcaa aggaatctaa aaaagaagta gttgtagggt ttgataatac gtttgtacca
    19381 atgggatttt tagatgaaaa gggagataca gtaggatttg acgtagactt agctaaagaa
    19441 acatttaaaa gacttggaat ggaagttaag tttcagccta tagattggtc aatgaaagaa
    19501 actgaattaa atgattcaaa aactgtagat gtattatgga atggatattc tataacagac
    19561 gaaagaaaga aaatagtatc atatacagaa ccatatttac aaaataagca gataatagtt
    19621 actctaagtg actcaaaaat aaattcaaaa gctgatttaa aagataaaga agttggaaca
    19681 caacaaggtt caacagcact tgatgcagtg gaaaaggata aagattttat gaatagctta
    19741 aaaggtggag caccagttct ttatgatact tatgataaag cacttagaga tttagagata
    19801 ggtagaacta gtgcagttgt tggtgatgaa gttttaatta gatactatat gggacaaaag
    19861 ggtgaagaca aatacaaggt attaaaagac gattttggat tagaagatta tgttgtagca
    19921 acttctaaag aaaatccaga actttgtgag aaaataaatg aaacattaaa agaaatgaaa
    19981 aaagatggaa cttttgacaa gatttatgat aagtggttta aatagaagat aagttagctg
    20041 ttaagaaggg ggcactaaag gcatgttaca aggtttagag atagttatag caatgttttg
    20101 tataacattg atagtatcaa ttccattggg gattggagtt gcatttttaa gattatcaaa
    20161 gaataaatta gtaagtggta ttacacaatg ttatatattg ataatgagag gtacaccttt
    20221 acttttgcaa atgatagtca tattttacgg attacctctt ttaggaattg tatttgacag
    20281 atttacagct ggagtagtag ctttcttctt aaattatgca gcatattttg cagaaatatt
    20341 tagaggtgga atacagtcaa tcgatagagg acaatatgaa gcttcaaaag tactaggatt
    20401 tgataagttt actatgtata agagagtaat ctttccacaa gtttttaaaa gaatactagc
    20461 acctatttca aatgaagtaa tcacattagt aaaagataca tctttagtat atatattagg
    20521 attaaatgat attttaagaa tatctcaaat agcaatgaac agggaagcaa gtctgttacc
    20581 attatttgag gcaggagcga tatatcttat atttgtagct atacttacta agggatttga
    20641 attacttgaa aaaaaatatt cttattatag ataaagttac aatattatag gaggaggttt
    20701 tttatatgtt gaaaatcaaa aacttaaata aatcatttaa aaaaaataga gtacttaaag
    20761 atatttcttt tgagttagaa gaaggtcaga ttggagtttt acttggtaag tctggtgcag
    20821 gaaagaccac aatattaagg tgtataaatg gtcttgaaga atttgatagt ggagagatta
    20881 taatagataa tgaggtaata aaaaataaga gagatatggc aaaaatacga ggaaaaatag
    20941 gtatggtttt tcaaaatttc aacttatttc ctcatatgac agtccttgaa aatataattg
    21001 aatccccagt taatgttttt aaagttccta gaaaagaggc tgaagagagg gctagagaac
    21061 ttttaagatt agttgattta gaagataaat taaattctta tccatttgaa ttgtcaggag
    21121 gacagcaaca gagagttgct atagctaggt catgtgcatt gatgccaaaa gtcctttgtt
    21181 ttgatgaacc tacttctgca cttgatattg atactattca aagagtagtc aatataatga
    21241 acagacttaa agacaaaggt atgacaatat taataattac acatgatgtt gtcttctcaa
    21301 ataatgttgc agataaaatt ataagtataa aagatggtat agtagaaaat gttcaaataa
    21361 aagaaaaaat tgtttagagt tttataaata taaactaggg tagaatttta taatataaat
    21421 aaagggcaag taaaatcaaa tatactatgc ccttttgtta tctcaaaatt taatcttata
    21481 ttttatgttt aagttacatt ataatttata ataaaaataa aaggactatt taaatatata
    21541 atgtgaataa tgtttaataa tttgaaatat gataattttt tatgttgaaa gcttataatg
    21601 attatgatat aaattggtta tagtatacaa tttatgggta taacaataaa aaattagaaa
    21661 ggaattttta taaaatgatt aaattatatg gatatacaaa atgctctact gttaaaaaag
    21721 ctaaaaattg gcttaaggaa aataatttag aatttgaaga tatagatatg gttcaaaacc
    21781 ctcctagtaa agaggagtta aaatcaattt ataagactag tggatatgat ataaagaaat
    21841 tttttaatac aagtggtatg aaatatagag aattgggtct taaagatatt gtaaaaactg
    21901 aatctgatga taaactttta gaaatattgg taagtgatgg tatgcttatt aaaagaccat
    21961 tacttttgga tggtaaaaat gtattattgg gttttaaaga agatgtatgg aaaagtactt
    22021 tgctaaaaga agattaggaa gagataggtg tgttgattaa aat
//
LOCUS       DAEMGG010000060        22022 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEMGG010000060 DAEMGG010000000
VERSION     DAEMGG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.060"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(195..386)
                     /locus_tag="KPK24_002140"
     CDS             complement(195..386)
                     /locus_tag="KPK24_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299812.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            606..1652
                     /gene="cobT"
                     /locus_tag="KPK24_002141"
     CDS             606..1652
                     /gene="cobT"
                     /locus_tag="KPK24_002141"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG2299813.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            1684..2244
                     /gene="cobU"
                     /locus_tag="KPK24_002142"
     CDS             1684..2244
                     /gene="cobU"
                     /locus_tag="KPK24_002142"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG2299814.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            2254..3027
                     /gene="cobS"
                     /locus_tag="KPK24_002143"
     CDS             2254..3027
                     /gene="cobS"
                     /locus_tag="KPK24_002143"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG2299815.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            3047..3661
                     /locus_tag="KPK24_002144"
     CDS             3047..3661
                     /locus_tag="KPK24_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG2299816.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      3728..3907
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4037..5536
                     /locus_tag="KPK24_002145"
     CDS             4037..5536
                     /locus_tag="KPK24_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG2299817.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            5575..6951
                     /locus_tag="KPK24_002146"
     CDS             5575..6951
                     /locus_tag="KPK24_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG2299818.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            6952..7923
                     /locus_tag="KPK24_002147"
     CDS             6952..7923
                     /locus_tag="KPK24_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG2299819.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFWEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            7955..9025
                     /locus_tag="KPK24_002148"
     CDS             7955..9025
                     /locus_tag="KPK24_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2299820.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEEREYML
                     QELRNIRNIKVYDTDANFILIKIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            9027..9914
                     /locus_tag="KPK24_002149"
     CDS             9027..9914
                     /locus_tag="KPK24_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG2299821.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            9902..10534
                     /locus_tag="KPK24_002150"
     CDS             9902..10534
                     /locus_tag="KPK24_002150"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG2299822.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            10543..11769
                     /locus_tag="KPK24_002151"
     CDS             10543..11769
                     /locus_tag="KPK24_002151"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG2299823.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            11766..12374
                     /locus_tag="KPK24_002152"
     CDS             11766..12374
                     /locus_tag="KPK24_002152"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG2299824.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            12364..12939
                     /locus_tag="KPK24_002153"
     CDS             12364..12939
                     /locus_tag="KPK24_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG2299825.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            12932..13684
                     /locus_tag="KPK24_002154"
     CDS             12932..13684
                     /locus_tag="KPK24_002154"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG2299826.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            13668..14789
                     /gene="cbiG"
                     /locus_tag="KPK24_002155"
     CDS             13668..14789
                     /gene="cbiG"
                     /locus_tag="KPK24_002155"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG2299827.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYHVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELVYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            14786..15511
                     /gene="cobJ"
                     /locus_tag="KPK24_002156"
     CDS             14786..15511
                     /gene="cobJ"
                     /locus_tag="KPK24_002156"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG2299828.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            15508..16260
                     /gene="cobK"
                     /locus_tag="KPK24_002157"
     CDS             15508..16260
                     /gene="cobK"
                     /locus_tag="KPK24_002157"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG2299829.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            16279..17757
                     /locus_tag="KPK24_002158"
     CDS             16279..17757
                     /locus_tag="KPK24_002158"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG2299830.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            17787..18692
                     /gene="hemC"
                     /locus_tag="KPK24_002159"
     CDS             17787..18692
                     /gene="hemC"
                     /locus_tag="KPK24_002159"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG2299831.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSAIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            18682..20181
                     /gene="cobA"
                     /locus_tag="KPK24_002160"
     CDS             18682..20181
                     /gene="cobA"
                     /locus_tag="KPK24_002160"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG2299832.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            20198..21163
                     /gene="hemB"
                     /locus_tag="KPK24_002161"
     CDS             20198..21163
                     /gene="hemB"
                     /locus_tag="KPK24_002161"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG2299833.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            21316..21894
                     /locus_tag="KPK24_002162"
     CDS             21316..21894
                     /locus_tag="KPK24_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG2299834.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT         9056 a         2119 c         4155 g         6692 t
ORIGIN      
        1 gataagttaa taatgttaaa tgggaagtga gtttatggaa aaaccaaata caaaagttgt
       61 taactttcaa gagtataaag acagaaaaaa ccaaagagaa gtaaatagag acgaactttt
      121 aaagcttata aaaaaaattg tgacttcaaa atagggagtt gttaaactcc ctattttaat
      181 gggtttcata ataattaaga cattctgagt ccatctatat aatctttgaa ttgtgagaaa
      241 tctgattcac tacttaattc tatggtttct gaaccttccg tataaaaatt cttaagatag
      301 atagtataat tattatcaac acaatcatga taaatacatc tatatccatc ttcatacaaa
      361 ctttttaact tagaaaaatc atccacaata tcatctcctt aaagttcttt taaatatatt
      421 tttacaaaaa gcatctaatt gtatgcaaat tgacaatgta aaataaagga aatgaacata
      481 aaatgtagaa taatttttat atgtatgtaa aaataaaaag aaaaagttga aatttttaga
      541 aaataagagt attttttatc taaaaagaga gtttaaaaaa taaaaaaatg ttgggaggat
      601 aaaaaatgag tttattagaa agtatatcaa aaaacatata ttccctagat aactcttcta
      661 ttgaaaaaac aaagcagaga ttggataggc ttatacatcc aactggaagc ttaggaaaga
      721 tagaagatat ttgcatgcaa ttagcaggaa tatttggcaa tgaaaatttt gacacaagta
      781 aaaaggttat aattgctttt gcgggagacc atggtgtata tgaagaaggc gtagcacctg
      841 accctcaaaa tataacaaaa ttacagtttc caaatttttc gaaaggtcta tgtggagttg
      901 gagtaataag taaatttgta ggggcagatg ttgtagctgt agatgtagga ataaactgtg
      961 atgaaaaact ggatggggta ttagactaca agataagaaa agggacatca aatatggcaa
     1021 aaggtccagc tatgagtaaa caagaagcaa ttagatgttt agagataggt attgaaatag
     1081 cagagcagtg tatagaaaaa gattataagg ttattggtat tggggagatg ggtatagcaa
     1141 atactactcc aagtacagca ataatatctg taatttcagg gtgtgacccg ttagaagtta
     1201 ctggtatagg tgcaggtctt aagaaagaaa gactaaagca taaggctgaa gtaattagaa
     1261 aggctattga aattaataat ccaaatccaa ctgatggagt agatatttta tctaaagtag
     1321 gagggtttga aataggctca atggcaggtg ttatattagg ttgtagtgct aataggattc
     1381 cagtagtaat tgatgggttt atttcttatg cagcagcatt aattgcatat aagataaatc
     1441 caaaaactag agaatatatg atagcatctc atttatcagc agaatcagga actaaaagag
     1501 cattagacat attaaaatta gaccctctat taaatatgga tatgagatta ggtgagggaa
     1561 gtggagctgc tttagcattt gatataatag aagcttcaaa ctatacttat aaaaatatgg
     1621 caacttttga tgaaattgac atgggaagat aaatataaaa atttaatatg taggagtgtt
     1681 ttgatgagta aaattattct agtaacagga ggagctaggt cagggaagag taactttgca
     1741 gaaaaactat gttttgacag aaataataat acagcttata tagcaacctc tattccattt
     1801 gatgatgaaa tgaaagatag agtaaaaaaa catcaagaga atagacctag aaactggaat
     1861 acttatgaaa tatatgaaga tatatattcc ataattaagg agatatataa agaacatgaa
     1921 actgttatac tagattgtgt aacattatta gtaaataatc taatgtttga acacaatcta
     1981 aatatagaag aatctagtca agaagaaata aacaaattag aacaatacat aaaagaacaa
     2041 gttgtcaaac ttattgaaga aatagaaaaa acaaatctat actttgtatt tgtaacgaat
     2101 gagttaggta tgggaattgt tcctgggaat aaacttagta gaatttatgc tgatattgta
     2161 ggtcgtataa atcaatatat agcttcgaaa agtaatgaag tttattttgt agttagtggc
     2221 atagctatga agataaagga gtagtatgca attatgaaaa gatttatttt aatactacag
     2281 tttttgacaa ggatacctat aaaattaaat gtaggatttg atgatgaatt ttataagtca
     2341 atagtatatt ttccacttgt aggttttgtc ataggtatat tatcctattt aataggatgg
     2401 atttctatgc ttttatttga accatttata gcttctataa taataactct agctggtgtc
     2461 ttaattactg gaggacttca tatagatgga ttaggagata catttgatgc aatatatagt
     2521 tatagggata aagaaaagat gcttgaaata atgaaagatt caagattagg tacaaattca
     2581 cttctagcaa ttatgtttgt acttttatta aaggttggat tcgtttacga tataataagt
     2641 aataattcat tgtgggttat aatttttatg ccaatgattg caaggttagg agtaatgtta
     2701 ttgacatata aaactgtgac accaagagaa aaaggaatgg gaaatttatt tataggaaaa
     2761 ttaactacaa gtatgttaat aactgctata atatacacat tattgatagt tgctcttatt
     2821 actaaattta tatttttatt acctaatata gtgttgatta aagtattagg ttcgattata
     2881 gtggtttttg tatttataat attattcaaa aaacatattt ataaaaaaat tgacggagta
     2941 acaggagata tattaggctg tggaatagaa ctttcagagc ttgtatattt aatttacata
     3001 tatttgttaa tttttatgtt tttttaaagt aaaaagtggt gataatatga taagattaat
     3061 attaataaga catgcactga caaatgataa taaaaaagga agattatcag gccatataaa
     3121 tagttgtatt agtgaagagg gcaaactaca aataaataaa attaccagat atctgagtaa
     3181 tgaaaacata gataaggttt atacaacacc atctacacgt actaaagata cagtagaaaa
     3241 aatatcaaaa ttgaaattat tagaaataga agaaaaagaa gctttaagag aaattagttt
     3301 tggagacttt gaaggaagaa cttttgaaga aattaaaatc aaatatccaa atgagtttga
     3361 aaaaatgata aaagaaggta acaactatag atatccaaat ggagaaagtt tgatagactc
     3421 ttataaaaga gtggcaaaag aaattgataa tataatttta gaaaatgata gtaatttaga
     3481 tacaaagaca atacttatat gttctcatgc aggaacaata agaaatataa taacacattt
     3541 gatttctggt agttataagt atcactggaa ctttaaaatt gataatgcat ctattacagt
     3601 tttagaaatt gatggtggat ttgctgtaat agataaaatg aattttacag acttcatata
     3661 atttaatcaa aaccaaacat tacaatctgt gtttgtaata tatctgtaaa attagcaatg
     3721 aaaatagaat attagattaa aattaggttc ttaattagat gttaaaaggg aaaaaggtta
     3781 gatgcctttg cagcccccgc tactgtgaaa ccaacgaaac tataaatacc actgtcagtt
     3841 tgatgggaag gttatagaag taggatgaag ttaagccagg atacctgcct aatttaattt
     3901 aaaacaagag gtcttcgggg tgaaagattt ttaattaata tatataaaag tcctatcttt
     3961 agaagtatta ggactttttt taatttgttt tttagtaaat tttaacaagg agggatggaa
     4021 agtggggaaa aaaataatgc tacaaggaac agcttcaaat gtaggtaaaa gtgtcttagt
     4081 agcaggtttg tgtagaatat ttaagcaaga tggttatact gttgctcctt ttaagtcaca
     4141 aaatatggct ttaaattctt ttataactaa agaaggatta gaaatgggaa gagcacaagt
     4201 atttcaagca gaggctgcga aaatagagcc aatagctgat atgaatccag ttttattaaa
     4261 accttctgga aataataaaa gtcaagttat tgtaagaggt aaagttgtag gagaaatgcc
     4321 ttcttctaag tatcatgatt ataaattgga gttagtagat gtgttgaagg aaacatttaa
     4381 tagcttaaat tcaaggtatg atattgtagt tatggaaggg gcaggaagta atgcagagat
     4441 aaacttaaaa gatagagata tctctaatat gggtatggca gaaatagctg atgcacctgt
     4501 aataatagtc ggagatattg atagaggagg cgtatttgcc tcattagcag gaacaatgct
     4561 tttattaaat gaagatgaga aaagaagagt aaaaggtgta ataatcaata aattcagagg
     4621 aaaaaaagag cttttacaag atggtgtaaa gatgctagaa gacatcataa aagtacctgt
     4681 tataggagta gtaccttata cagatataaa gatagaagat gaagacagtg taactacgag
     4741 atttaagaaa aaaatgaata aaagtgatat acacatagaa ataattagaa ctccacatat
     4801 gtcaaatttt acagatttta atatatttga gacacaagaa gatgtaagta ttagatatgt
     4861 agactatgga gaatctttag gaaatccaga tatattaata atacctggaa ctaaaagtac
     4921 tatagacgat ttaacatata taagaaaaag tggtcttgaa tcgcaaatca aaaatttaca
     4981 tagtcaaggt aaattgatat ttgggatatg tggtggatac caaatgttag gtaaaaaatt
     5041 aaaagaccct tatcatgttg aaagtgaaat tgaagaagtt gatggaatag gtcttttaga
     5101 tacagaaaca acgtttgaga aagaaaagac tacaacacaa gtagaagcta ctattgttga
     5161 atgtattagt gaatacatga ataaccttaa gggaaaaaaa gtaaaaggtt atgaaattca
     5221 tatgggaatt acaaagcgag gtaatgatgt aagaagttta gatttaataa ctaaaaaact
     5281 tgataaagaa gtgaattata ctgagggaag tgtaaataaa gaggggaatg taattgggac
     5341 ttatcttcat ggaatatttg atgaaataag ctttacaaga gcaattttaa acaatataag
     5401 aaagaaaaaa ggtttaggag aattagaaag caaagtaaac tcttttaatg aatttaagga
     5461 taaagagtat gataagttgg ctgatttttt aagagaacat ttagatatag agaaaatata
     5521 tgagattatg aaataatgat gacctttatt tataatttag gtaggaggtg tattatgaaa
     5581 aaaattttaa ttgcgggtac taatagtgga gtaggtaaaa caacaatatc attaggaata
     5641 atgcaagctt tggttaagag aaatatgaaa gtacagccat ataaagtagg gccagactat
     5701 atagaccctt catatcacac ttttataact ggaagacatt ctagaaattt agattcttat
     5761 atgttagatg atgaaaaaat aaagtatata gtaaataagt cctcaaaaga tgcagatatt
     5821 tcagtaatag aaggtgttat gggcttatat gatggctttg gaatagattt agataattgt
     5881 acaagttcac atacttcaaa ggtattaaag tcacctgtta tattagttat aaatggtaaa
     5941 tctatggcag catctagtgc tgctatggta cttggatata aggaactaga taaagatgta
     6001 aatataaagg gtgttatagt aaataatgta aagactaata gccattatca aataattaag
     6061 agttctatag aaaaatattg taatgttgaa gtattggggt attttcctcc aaataacaaa
     6121 ttctctttgg aatctcgaca tctaggatta gttcctagtg tagaaatgaa atcactaagg
     6181 gaaaaatttt ataccttagc agatgaaatt gaacactata tagatattga cagaataatt
     6241 gaaatatcag aaagcgatga atttgagagt gattttgatt ttcaaacttt aatagaaaat
     6301 aataaaataa aagaacatgt taataataaa tcaatagcta tagcatatga taaagcattc
     6361 aatttttatt atagagaaaa tatagagctt ttagaggaac ttggtttaga aataaactat
     6421 tttagccctc ttgaagatgt atcagtgcca aatgcagatt atatttatat tggaggaggt
     6481 tttccagaaa tatttggaaa agaattagaa gcaaatgagt caatgagaac ttccatatta
     6541 gaagcacata acaataatat acctatttat gcagaatgtg gaggactgat gtacctaggt
     6601 gagaaactat tgaatcaaga agaacaagaa tttaatatgg ttggaatatt tagtggaatt
     6661 agtaagatga catcttcttt aaaaagattt gggtactgta ttggtgtagc caaggaaaat
     6721 actcttttat ctaaaaaagg ggaatctatt aaagggcatg agtttcatca ttcaatattt
     6781 gaaagtgatg aaaaatgtgt atattcaatg agaaaagaaa gagatggaaa tatagtagaa
     6841 gagtgggatg gtggttatag taaaggaaac actttggcta catatcttca tactcatttt
     6901 tataataact taaattgtat agaaaatttt ttgaaaaaag gaaatgaata aatgagtatt
     6961 ttatctattt atataggata tgtgacagac ttaattgtag gagacccata ttcttttcct
     7021 catccagtga gatttattgg taaacttata aattttactc aaggtataat aagaaaggtc
     7081 tctaaaaatg ataggcagtt aaaattgggg ggatttatat tatggtttat aaccgttgga
     7141 ataacatatt taacaactta tgctatagtg aagttattta gttttaatat attattgagt
     7201 attgtagtga atagttttat aatttataca acactagcca caaaatgttt aaaagacgaa
     7261 gcattaaaaa tttataatgt attaaaaact gaagatatag aaaaatctag gattcagcta
     7321 tcttatatag taggtaggga tactactaat ttaagtgaac ctgaaataat aagagcaact
     7381 gtagaaactg ttgcagaaaa tactgtagat ggtataattg cgcctatgtt ttatgcattt
     7441 attggaggag caccacttgc tatggcctat aaagctatca atacattgga ctcaacagtt
     7501 ggatataaaa ataaaaagta taaagatatt ggttttgcat ctgctaagat tgatgatata
     7561 gccaattata taccagctag aatatctgtc attcttatga caattggaag tttcttttta
     7621 aattataact ataggaactg ttttaaaatc tctattagag atagaaagaa tcataaaagt
     7681 ccaaattgtg ctttttcaga aggtgcagta tctggtgctc taggaataca actaggaggt
     7741 accaacgttt atttttggga aaaagtatat aagccaacaa taggggataa gcttagggag
     7801 atagataaag aagatataat taaaactaat aaaattatgt atgcatcttc atttgtatct
     7861 atgttggtat ttacactaat atatgtgtta tgtaggatga ttttaattaa cttaattata
     7921 taaattattg tttgttaact taagggggac atagatgaag gatttagggc atggtgccaa
     7981 tgtagatgaa atggcaagat tatatgggaa aaacccaaaa gaaataattg attttagttc
     8041 aaacataaat ccaaatgtat taccaaattt agagagatac attttaaaag gattagaaga
     8101 gtgtaggaat tatccagata taaattatac aaacttgaga gaaaatatat caaaatatat
     8161 agatataaat ccagacttta taatacctgg caatggtgca acagaagtga tttatttgct
     8221 tatgaagagt attaaaaaaa aattggctat aataaatccg actttttcag agtatagaag
     8281 aagtgctgag ttaaataatt tagatattat agatttagag ttagatttag aaaataactt
     8341 taaacttaat atagatataa taaaagaaaa tataaaaaga tttgatagtt tatttatatg
     8401 taatcctaat aatccgagtg gtaatgtcca agatttaaag gaacttgttc atttactaga
     8461 taaacataat aaggtattaa taattgatga aactttcatg gaatttgttg aagatgaaag
     8521 taaatatagt ttggttaaat atatagaatc aaataaaaat atatttataa ttaaagctgt
     8581 gactaagttt tttggtatgc ctggtttaag gctaggatat ggtcttacaa gcaacactga
     8641 aattatgaac aaaatttatg aacacaaaga gccatggact atcaattctt ttgcagatat
     8701 actatccaat tttatttttg aagataaaga gtatattaaa aatagtaaag aatattacat
     8761 agaagaaaga gaatatatgt tacaagagtt gagaaacata agaaatataa aggtctatga
     8821 tacagatgct aattttatct tgataaaaat atataaaaaa acaactaaag aattgaaaaa
     8881 agatttgttt aagcaaggaa acatattagt tagagatgcg tctaatttta taggattaga
     8941 tgatagtttt ataagagtgg ctataaaatc tcatgaagat aacaaaattc ttatagaaaa
     9001 tatgaaaaat ttattgggtg attaaaatga aatcttatgg aatatgccca gcatcttgtg
     9061 gagaatttgt acaaggtata attgatgatg aagaatattt atgttcatat gcaatagata
     9121 tgtattctaa agtgtatatt gaagaaaaat tagtagatat aaatttaggg agatataagt
     9181 caaggttagc aattgaaaag gtatttgaaa aatttaattt acctaaaaaa tatactaaaa
     9241 atatttcttt aaatataaat agtaaaatac ctgttggaaa aggaatggca agttcaactg
     9301 ctgatatagg agctactata aaggcaacat tatcattgat agataaagat ttaagtagcg
     9361 aggagatttc taaattagca gcagaaatag aaccaacaga ttctatattt atagataaaa
     9421 atagtatatt taatcctcta aatggaactg ttataaaata cctaggaaat ctgaccaatg
     9481 cgaaagttgt aatacttgaa ccaaacaaag tactagatac tatgaaaatt agattgaggc
     9541 aagattataa taaattaaaa gtagagaata aagaagtaat aaaaaaatca tttgctcttt
     9601 tagaagaagg attgaaaaaa aataatttat ctctagttgg agaggcttgt actttgagta
     9661 gcttagcaaa tgagaatata gaaaaaaaag agtatttaaa tgaaataata aaaatatcaa
     9721 aaaaatatgg agcatatgga gtaaatattg ctcacagtgg aactgtagta ggtattttaa
     9781 ttgataagtt tatgaatgat aaaaagatga tagatgcctt atgtgaatct aacataaatt
     9841 ctgtctataa taaaatttat acacaaaaca taattaatgg aggaattaag ggggaaatag
     9901 aatggaatac ataaagaatc ctatgaaaat agaagaaaaa agttttgaat tgatacaaga
     9961 aataatagac gaaattagac cagattataa atttaagaat aatatcgaag aaaaaataat
    10021 aaaaagagct atacatacta ctgctgattt tgattactta gatatattaa aaatatcaga
    10081 agaagcagtt gatagtataa tagatgcatt aaaaaataat gcaagtattt atactgatac
    10141 aaatatggct cttagtggta taaataaaag aaagttagaa tcattaggat gtaagtataa
    10201 atgtttagtg gcagatgatg aaactgtaaa attagctaaa gaaaaaggaa ttactcgttc
    10261 tatggcagca gtagaaattg cagctaaaga agaaggaaga aaaatattcg ttttaggtaa
    10321 tgctccaaca gctttatata aagttatgga gatgaaatca gaaggtagat tagatttaga
    10381 tgcagttata ggagttcctg taggttttgt aggagcacaa gaatcaaaag atgaagttga
    10441 aaaaacagat ataccatata taatttctaa aggtagaaaa ggtggcagta atttagcagc
    10501 agcaatagta aatgctatat tatatactat gtaggtacct atatggaaga atatgtatat
    10561 atagatggaa aaaaatatag aagaggatat actacaggtt catgtgcaac aggtgcatct
    10621 aaggctgctg tatatatgtt gattacaaaa aatagaatta atactataaa tatagatact
    10681 cctaagggga taccattact actaaaagtt gataatataa atatttctga tacatttgtg
    10741 gaatgttcca taaaaaaaga tggtggagat gatatagatg caactcatac catggatatt
    10801 tatgcaagag ctgaaattgt agctaaaaat gataagaaca aagggtatct tactctaaag
    10861 gatatagata gtttaagcac caatagtgag tgtaaaagtg agctctataa gtttattagg
    10921 gtttatggcg gaactggtat tggtgtggtt acgaaaaaag ggcttagtgt ggatgttggt
    10981 aagcctgcta taaatccgac tccacttaaa atgataaatc atgaaataag aaagcttata
    11041 ggagataatt ttgaatctat acttggaaat gataaagttt taaaaataac aatctttgct
    11101 ccacaaggag aaactgtagc aaaaaaaaca tttaatccaa gattaggtat agttggagga
    11161 atatctataa taggtacaac tgggattgtt gaacccatga gtgatgatgg ttggaagaag
    11221 tctctatcta tagaacttca gatgaaaaaa gaacaaggct tagataaaat aatattggtt
    11281 ccaggaaatc atggagaaca gtttataaga gaaaaattaa acttagatat taagtatgta
    11341 gttagggtaa gtaattttat aggctatatg attaaagaag ctcaaagaat aggatataaa
    11401 aaaatcttaa tggcaggcca tataggtaaa tttataaaag tatctgctgg tatttttaat
    11461 actcatagta aggtagcaga tgctagaagt gagatattag tggcaaactt agctttaatg
    11521 ggagctagat acgaattttt aaataaaata aatcaatgcg taactactga agaagctgta
    11581 gagctaataa ataatagtga atatagagaa gtatataata tattaagtaa taaatgtagg
    11641 gagagagtaa agcaatactt gaatgaagat tcagatgata ttgatgtgga agtaatcata
    11701 ttttcaatgg ataagtcttt attaggtaaa tcggataata cagatgattt agtggaggtc
    11761 tttatatgat aaacataata ggtctaggac ctggtaactt agactatata acaaaaaaag
    11821 gtgaaaattt aatttccact tctgatgtat taattggtgg taaaagaaat ttagagtcta
    11881 taaaaaattt tgaaggagaa aaaatagttc tggattctaa tttaagagaa attatagagt
    11941 acataaataa caataaagaa aagcaaatat caataatagc atctggggac ccattaatat
    12001 atgggatagg tagatatcta tctaaaaata ttgataataa gatgttaaac atggtttctg
    12061 ggattagttc attacagtac atattttcaa aaatatatgt agatatgaat gatgtatata
    12121 ttacaagtag tcatggcaaa gtaccagatt ttgattacat attatcacat aaaaaagtat
    12181 gtatggttac agattcaaag ataggaccaa aacaaataag tagagaaata atagatagaa
    12241 atctaaataa aattattgtt gtaggagaaa atctgtcata tgacaatgaa aaaataacaa
    12301 tagcaaaacc agaagagata ataagaatgg acaattttga tatgaatgtt gtggtgattt
    12361 tagatgagga atagtgaatt tataacaggt aaagttccaa ttactaagga agaggttaga
    12421 gcaatatcaa taagtaagtt aaatttagta aatgctaaaa acctcataga cataggggct
    12481 ggtacaggaa gtgtaagtgt tgaagctgca tataattatc ctaatttaaa ggttatatct
    12541 atagagaaaa atgatgatgc tatagagctt atagagaaaa ataaaaaaaa attcaactta
    12601 aaaaatatag aagtgataaa aggatatgct ccaattaatc tagggttaaa tgttaaagtt
    12661 gagtctattt ttcttggggg aacaggaaat aatctggaag aaataataga atggtctaaa
    12721 aaaaatctcg ttacaggagg aagacttgta gctaatttta taataataga tacatttaat
    12781 caaactttaa aactgcttaa aaagcatgga tttaaagaaa tagatgtatg tgttttgaat
    12841 gtttctaaac tagagaaact aggaaaaggt gaatatttta aaccactaaa tcctatatat
    12901 ataatatctt gtgaaaaagg ggaaaatgat gatgaataaa gtacattttg taggagcagg
    12961 acctggagat aaagagctga taacattaaa aggatacaaa ttattaagta atgcagatgt
    13021 agtgatatat gcaggttcac ttgttaatcc tgaacttttg gaatactgta aagaagattg
    13081 ccaaatacat aatagtgcac atatggattt acaagagata atagatgtta tgagagaagg
    13141 tatagaaaat aataaatctg ttgtaagact gcaaacaggt gatttttcta tatatggttc
    13201 aattagagaa caagtggaag atttaaataa attgaatata gattatgact gtactccagg
    13261 agtaagttct tttttagggg cagcatcatc tttaggggtt gaatatacag ttccagaaat
    13321 ttctcaaagt gtaattataa caagaatgga aggcagaact ccggttccag agaaagaatc
    13381 aatacaatca tatgctaagc atcagacatc tatggtaata tttttatctg ttcaagaaat
    13441 tgagaaagtt gtgtctaaat tactggaagg tggatatcca aaagatactc ctatagcagt
    13501 tatatacaaa gcaacttggg cagatgaaaa aatagttaaa ggaactttaa gtgatatagc
    13561 tgtaaaagtt aaagaaaaca acataaataa gactgcttta ataatggttg ggagattttt
    13621 aggagaagag tataataatt caaagttata tgataaggat ttcaaacatg aatatagagg
    13681 gtaatattgc tataatctgt attacagaaa atggaaaaaa attagctcta aagataaatt
    13741 ctctagtaaa taactgtgtt atatatcatg taaaaaataa aaaaagtaat ttagttgttg
    13801 aagaaacatg tgtgcataca gttcaaaaga agttaagtga ttttgtagga gaaatattta
    13861 gcaaatttga ttacatagta tttataatgg ctacaggtat agttgtacga agtatagcac
    13921 catacataat aagtaagttt agtgaccctg caattttggt aatggatgaa aaaggcaaaa
    13981 acgtaataag tttattgagt ggtcatattg gaggggcaaa cagcttgaca cagtacataa
    14041 gtagtcttat tgatgctaat ccagtaataa caacagctac tgatataaat caaaaagctt
    14101 ccttagatat gatagcaaaa aaactaaatg cacatattga taattttaga gaaagtgtaa
    14161 aagaagtaaa ttctatgcta gtgaataata aatgtgttgg attatatatt gatgaaggat
    14221 atcaagttga tactagggga tttaaactta tagaaagtaa ggatgagtta agagatagtt
    14281 tttcaaacac atatatagaa aaattagttg tagtaagcaa taaaataaat tgtattgaag
    14341 atattgagaa tttaaaccta gataataaat ttgaacattg ctatagtacc aaaaaaataa
    14401 taaaggtaat tccaagagat atagtgatag gaattggttg tagaagaaac acagatagtg
    14461 aactggtgta taattcactt gaagagtttc ttaataaaca aaatatagat attagagcaa
    14521 taaacacagt tggaagtata gaactgaaaa aagacgaaaa agcaataatt gaattatcaa
    14581 aaatattgaa ggtgccattt aaaacatttt cagtagatga aatatctaag gttgataatc
    14641 tttttgataa atctgatttt gttaaaaaaa atgtgggcgt gtattcagta gcagaacctg
    14701 tagcatatct tttgagcaaa ggtaatttgt tagttgaaaa acataaatat aaaggaataa
    14761 ctttttcagt ggggagaata aaaatatgat atacgttgta ggaattggac caggaagtaa
    14821 agatacaatg actttagaag caataaaagc aatagaagat tctgaggcta ttgttggata
    14881 taagacttat ataaaactaa tagaagaatt tatacaagat aaagaaataa tccaaaatgg
    14941 tatgaggcaa gaagttgata gatgtaaaca agcagtagag gaagcaaaaa aaggaaaaaa
    15001 agtagcagtt gtaagcagtg gtgatgctgg aatatatggg atggcaggtc ttattttaga
    15061 attaatatct aaagaagatg aagatataaa agtaaaggta attccaggtg ttactgcaag
    15121 tattggtgca gcagctatac ttggagctcc tattatgcat gatttctgtc atataagttt
    15181 gagtgattta atgactccgt gggaagtaat tgaaaaaaga ttgagattgg ccgctgaagc
    15241 cgattttgta atttgtttgt ataatccaag aagtaaggga agaagtgaac atctaagcaa
    15301 agcatttaaa ataatgggtg agtttaaagg tggagataca cctgttggag tggtaaaaga
    15361 tgtaggtaga gaaaaagaag aaaagtttgt atgtactttt gaaaatatgg actttgaaaa
    15421 agtcgatatg actacaatgg ttataatagg aaataagtct acctatatta atggtgaatt
    15481 gatgataact ccaagaggtt atacagtatg attttaattt taggaggaac ttcagatagt
    15541 ttatctatat gtgataagat aaatgaactg aaaaatcaac catatatcct ttcagttact
    15601 acagattatg gaagggaact tgcacaacaa catgcagaga atgtaatttt aggtaagctt
    15661 ggaaaagaag atatgctaaa atttataaaa gataataata tagtaaaaat aatagatgcc
    15721 actcatcctt atgcagttga agtatccaaa actgctatta cttgtgcaaa actattgaat
    15781 atagattata taaggtttga gagaaaatct ttaattgaag aaatagatta tgataataaa
    15841 tttatagtag atacaattga agaagcctgt gaaatagcaa ataaaatagg gacaaatata
    15901 ttcataggaa caggaagtaa aaatttgaac agatttatag aggaaataag tgataaaaat
    15961 ttagttgtta gagttcttcc aacgagtgaa gtcatattaa gttgtgaaaa attaggttta
    16021 agtgcagata atatagtagc tatgaaaggt ccatttagcc agtatataaa tgaagaaaca
    16081 tataaacatt acaatataga tttagtcata acaaaagaaa gtggaatagc tggtgggttc
    16141 ttagagaagg taaatgcatg taaaactttc aatataccag taataattat aaaaagagaa
    16201 tcgattaact acgaaaatgt atttaatgac ataaataaaa ttaaagacat acttgtgtaa
    16261 tttttaggag gagaatatat gaagaaagcc ttattagtag tgagttttgg tacaagctat
    16321 catgaaacga gagaaaagac aatagatata tgtgaagata aaataaaaaa ttcgttaaaa
    16381 agccatgatt tttttagagc ttatacatca aatatgataa taaataaaat aaaaaaaagg
    16441 gatggaatag aaatagataa tccaatacag gctttagata aaatatatga acaaggatat
    16501 gatgaagtaa taatccaaac acttcatatt atatgtggcg aagaatttaa caaattaaaa
    16561 gaacaagtag atggatattc ttctaaattc aaaaagattg tattaggaag acctttgctt
    16621 acacatattg aagattatca ggaagctgta gaggcaatta agcatcaaat tcctcatatg
    16681 gaaagtaatg aagcagtagt atttatggga catggaacac ttcatgaatc acattcagcg
    16741 tatccagcat tggaatacat gcttagggat aatggaataa atgcttatgt tggtacagtt
    16801 gaagggtatc cagaaataga acatgtaata agaagattaa aagaaggaaa tataaaaact
    16861 gtaaatttga tgccatttat gttagttgca ggagaccatg ctataaatga tatggcagga
    16921 gatgaagaag attcttggaa aacaatatta gaagaaaatg gatttaacgt aagagttcat
    16981 ttaaaaggac taggagaaaa ttcttatata caagataaat ttgttcgtca tgctgtaaaa
    17041 tgtgcagaga atgcgaagtt ctatggtata ggagcagggc caggagatgg ttctttactt
    17101 acgataaaag cggttaatac tttaaagaaa ctggatattc tatacactcc agaatcaaaa
    17161 aagggtggag atagtctagc tttatcaata gttagtgaat atttacctga atcagtggaa
    17221 ataaagtcta ggcatttccc aatgagtttt gatgggaatg aaaaaagttt agcttgggac
    17281 aatgtggcac aagaaattat atcagatgtt aatgatggta aaaatgtagg atttgttaca
    17341 ttaggagacc ctatgattta tagtacatat gtatatataa tgaaaagaat attaggagag
    17401 gtagaagtcg aaactatacc aggaatttca tcattttcca atatagcatc taatcagaat
    17461 tttccattag tcatggataa agaagcttta gtggtaattc cttgtactat ggaagatgat
    17521 aagatagagt atgctttgca aaattataat tcaatagtat tgatgaaagt atataaaaat
    17581 tttaaagaaa taataaaaaa attagaaaag tatgatttaa tagaacatgc tattttagta
    17641 agtaattctt cccaagaaag cgaaaatgtg tttacagatt taaaagaagc gcaccttgaa
    17701 gataagatat cttatttttc tactatacta gtaaataaag ataataaaat aaaataggtg
    17761 aaagcaatat ttaaaggaga cttaatatga atatagtggt tggaactagg ggtagtaatc
    17821 tagcgcttat acaaacagaa tgggtaataa atgaattaaa aaagaagtat ccagaaatta
    17881 gttttgagat taaaatcata aagacaaaag gagatttaat tcaaaatgtt tcactggata
    17941 aaataggtga taaaggattg tttgtcaaag aaatagagca acaattatta gatggaaaaa
    18001 tagacatagc agtacatagt atgaaagata tgccttctta tttagcagat ggactaaaat
    18061 ttgctcatac gcctaagaga gaagacccaa gagatgttct aatacttaga gaaggatata
    18121 aaaatttaga tgatttacct catggagcag taataggaac aggaagtaaa agaaggaagt
    18181 ttcaattatt gaaacaaaga cctgatttaa atatagtaca agttagaggt aatgtggaaa
    18241 ctaggatacg aaaaataaaa gatgaaaaca tgcatggtat tgtgttggca gcatcaggaa
    18301 taattagagc taatttgcaa gataaaataa gttcttattt acctgttgat gttgttattc
    18361 cagcacctgc ccaaggagct ttagctatag aaattagaag caatgatagt gcgatagagg
    18421 gaatagtaaa ttcactgaag gatgagaata cagaaatcca aatattggct gaaagaggtt
    18481 ttttagatgg tgtaaatgga agttgtcata ttccaatggc agcttattgt gaaataatcc
    18541 aagataaaat acatcttact ggcttatatg gagattcaga aggaaaaaaa gtagtaatca
    18601 agtctataga tggagatatt tcttctccta gagagttagg tttaaagtta gctaaacttg
    18661 tattgaagga gtatgagaat tatgaagggt aaggtttact tagtaggggc aggaccaggt
    18721 gattataaac tcatgacttt aaaaggttta gagtgtataa gaaaatcgga tgtaatagta
    18781 tatgatagat tagcaaatag tagttattta agagaagcta agcctgattg tgaacttata
    18841 tatgtaggca aggcatcaag taatcatatt ttgccacaag aagatataaa tagaattata
    18901 gcagaaaaag ctaaagaagg aaaaattgta actcgtctta aaggaggaga cccttatgta
    18961 tttggcagag ggggagaaga agcagagact ttagtagatg aaggtataga atttgaagta
    19021 gttccaggta taacttctgc tataggaggt ttatgttatg cgggtattcc tataacacat
    19081 agagaccatg cttcgtcatt tcatgtaata acaggtcatt tgaagggtga tgatagtgga
    19141 gaactaaact ggaatgctct tgcaaataac aaaggtactc ttgtgttttt gatgggaatt
    19201 tcaaacctag agaaaataag tgaaaactta atgaaagaag gaaaagataa ggatacacct
    19261 gttgctctta taagttgggc aactagatat aatcaaagag ttgttacatc tacacttgaa
    19321 aatgtttatg aaactgctat taaagaagaa gtaaaaccgc caactcttat agtagttggg
    19381 agtgttgtag gacttagaga aaagttgaac ttctttgaaa gtaagccttt atttggaaaa
    19441 agtattgctg taactaggtc tagaaatcaa aatagtgtat tggtagaaaa aataatggat
    19501 ttaggtggaa atcctataga aatacctact attaaggtag aaaaaataca aaataatatt
    19561 aacttagaaa atgaaataaa aaatataaat aagtataatt atctaatact aactagtaaa
    19621 aatgccgtag aaatcttctt tgaaaagata tatgaaatga attttgattt aaggatatta
    19681 tctaatttaa aggtatgtgc tataggttct gctacatcaa atgagttgaa aaaaagagga
    19741 attatagcgg atatagtacc aaaaaaattt gtagcagagt cactgtatga agagttagcg
    19801 cctattttaa atgatgacga taatatactt atacctagag cagaaaatgc tagagattat
    19861 ctggttgaca aacttaaggc tatttgtgaa gtaacagaag tacatatata tagaacagta
    19921 atagatgagg agaaaaaagg tgagatatta gatattttaa attctaatga catagattat
    19981 ataacattta ctagttcctc aacagtaaat aatcttgttg aaattatagg aaaagaaaat
    20041 attaaaatat tagaaaaaat aaaaacacta tctataggtc ctataactac agagacaata
    20101 aatagtttgg gaataaaatt gtacaaagaa tctgctacat ctactataga ttcgttagta
    20161 gatttaatta taaaaaatta attttggagg cttgaatatg ttaagaagac caagaagact
    20221 aagagcaagt aaagcaatca gaaatttggt aagagaaact aaggtgaatg tagaagactt
    20281 aatatatcca ctgtttgtag tagaggggga aaatataaaa gaagaaatta aatctttacc
    20341 agatgtctat catttatcaa tagatatgct agaagacgaa ataaaagaaa taagtgagtt
    20401 gggaattgaa catataatgt tatttggtgt tccagatgaa aaagatgagt gtgcttcaca
    20461 agcttttaat gataatggaa tagttcaaaa ggcaattaga aaggtaaaag aaataaattc
    20521 agatataaat gtaataacag atgtctgtat gtgccaatat acaagtcatg gacattgtgg
    20581 gattttaact gatactggtt atgttgataa tgataaatca ttggagtatt tggctaaaat
    20641 atctgtaagc catgctaaag caggagctga tatggttgct ccatctgata tgatggatgg
    20701 aagaatacaa gcaataagag aagctttaga tgaaaatgga ttagaaaatg ttggaatcat
    20761 ggcttatagt gttaagtatg cttcaaatta ttatggacct tttagagagg cagctcattc
    20821 agcaccatct tttggagata gaaaaacata tcaaatggac ccagcaaata gcaatgaagc
    20881 tataatagag actgaacttg atatattgga aggtgcagat atacttatgg ttaaacctgc
    20941 attatcatat ttagatatta taagaagagt taaagataat tttgacttac ctctagcagc
    21001 ttataatgta agtggagaat actctatgct taagtttgct gtgaaggaag gtttattaaa
    21061 tgaagatgta attcttgaat caattacatc tataaaaaga gcaggagcag atataataat
    21121 aacttatttt gctaaagaca ttgctaaact gttgaaaaaa taaattctag cagtataaag
    21181 aatatcaaaa agtataggaa ttgtaattaa ctattataca gtagttttgt ttagacattt
    21241 attgaagaat aatagaaact aatttgcaat ttctatactt tatttatgta actaaataaa
    21301 atttggaggt agaagatgct atatcctatt aatttaaaat tagatgagtt agatgtagtt
    21361 ataattggtg gtggagaagt tgcatatagg aaatgtaaaa attttttaga gtttaacaaa
    21421 aatgttacta tagtttctaa acaaatttta aataaatttt atgatttaaa aggaaatata
    21481 aaaataataa aagatgatta taaagaaata tatataaaag atgcttctgt aataattgct
    21541 gccacaaata atagagagtt aaatatggaa ataggattat attgtaaagc aaaaaataag
    21601 ctagtgaatg tagttgataa tatagaaata tcgaacttta ctgttccatc atatataaaa
    21661 agaggagatt tacttataag tgtgtctact gggggcaaga gtccttcatt atcatcaaag
    21721 ataaaaaaag aaattgaaga gagatatact gaagattatg aggagtattt aaatgtatta
    21781 ggagatataa gaaaaaaggt tataaaaaaa tacgaagata aaagtaaaag aaaagatgtg
    21841 cttaatatgt tggtaacact tgacctagaa gaacttaaga agtttgatat ataaaaaata
    21901 aaataaaaaa tgttaaaaag gtgttgacgg attattttta agatgatata gtattacttg
    21961 tccttaagaa aagggcaaac ataaaaaatg aactttgaaa attaaacagt aggttaattt
    22021 at
//
LOCUS       DAEMGG010000061        21577 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEMGG010000061 DAEMGG010000000
VERSION     DAEMGG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.061"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            523..1731
                     /locus_tag="KPK24_002163"
     CDS             523..1731
                     /locus_tag="KPK24_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299835.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIIPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            1762..3141
                     /locus_tag="KPK24_002164"
     CDS             1762..3141
                     /locus_tag="KPK24_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2299836.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            3247..4437
                     /locus_tag="KPK24_002165"
     CDS             3247..4437
                     /locus_tag="KPK24_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2299837.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSKNGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGSVPNAIPNKASMSGTLRTFDQSVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(4565..5893)
                     /locus_tag="KPK24_002166"
     CDS             complement(4565..5893)
                     /locus_tag="KPK24_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299838.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(6513..7727)
                     /locus_tag="KPK24_002167"
     CDS             complement(6513..7727)
                     /locus_tag="KPK24_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG2299839.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            8153..8869
                     /locus_tag="KPK24_002168"
     CDS             8153..8869
                     /locus_tag="KPK24_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG2299840.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDKAATKFGLE"
     gene            9004..9630
                     /locus_tag="KPK24_002169"
     CDS             9004..9630
                     /locus_tag="KPK24_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2299841.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(9830..11374)
                     /locus_tag="KPK24_002170"
     CDS             complement(9830..11374)
                     /locus_tag="KPK24_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2299842.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(11400..12041)
                     /locus_tag="KPK24_002171"
     CDS             complement(11400..12041)
                     /locus_tag="KPK24_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG2299843.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            12311..13462
                     /locus_tag="KPK24_002172"
     CDS             12311..13462
                     /locus_tag="KPK24_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG2299844.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEVAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            13475..14287
                     /locus_tag="KPK24_002173"
     CDS             13475..14287
                     /locus_tag="KPK24_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2299845.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLSKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            14313..14804
                     /locus_tag="KPK24_002174"
     CDS             14313..14804
                     /locus_tag="KPK24_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2299846.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            15068..16471
                     /locus_tag="KPK24_002175"
     CDS             15068..16471
                     /locus_tag="KPK24_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2299847.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            16443..18143
                     /locus_tag="KPK24_002176"
     CDS             16443..18143
                     /locus_tag="KPK24_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2299848.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            18738..20129
                     /locus_tag="KPK24_002177"
     CDS             18738..20129
                     /locus_tag="KPK24_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG2299849.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            20143..21330
                     /locus_tag="KPK24_002178"
     CDS             20143..21330
                     /locus_tag="KPK24_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2299850.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
BASE COUNT         8076 a         2522 c         3745 g         7234 t
ORIGIN      
        1 gtaatagtct ctaggaataa gatatctaaa ttcgcttaga aatagtctaa tttacaaggg
       61 aggggatgca ctaatagata aaatttctat ataaagaaga cttaaagtga agtatattct
      121 gataatattg gtttaatggg tatattatta tttcgtttta gatattttaa gaataattta
      181 agacgtttat aaaaatatat tctatatcta atatttaaat aaatataagt cttccaacat
      241 gtatactaat atagagaata gttgaagtac aattctaact cgtaaaatta tgtagaaagt
      301 aaaaatcaaa aagaaatcta gtaagttgat aactatagtt aatatttaga gagttaactc
      361 tagttatatt tatttagata ttttaggaat aatttaggac gtatataata tcatcaaaaa
      421 atatcaagct ataccatatg tagttaagaa taactgcata atatacattt tataattatt
      481 gttttattga taaaaaatta ttatatttag gaggaaacaa caatggaaaa tttttggaat
      541 ccacttactt gctttactgt atgtgtaatg atttatgctt ttagtgagtt tttatctaag
      601 aaaacaaaag gtgcaatttc aacattgtta tttgtatgta ttatattttt actaggattt
      661 tggacaaata ttttaccaaa agacattaca ggagcatcag ggatattagc tgcaatgagc
      721 aactttggta tagctttatt aataactaac ttaggaacac ttataaatct tgaagactta
      781 tgtcatgaat ggaaaacggt tgtaattgca ttagtaggta ttgtaggaat aggggttggc
      841 tgtttaacag tgggtagtat gttatttggt agagagtatg ctttaatagc agcacctcca
      901 atagcgggtt ctactgttgc tggtattatc gtaacaactg ttgcagaagc tgcaaataga
      961 cctgaacttg ctgcatttgt aattctagta ttatcatttc aaaagttctt tggcattccg
     1021 ataactacat attgtataaa taaagaatta aaatttaaaa gagataacgg ggattttttg
     1081 aaagatgagt caaaaagtaa ttttaaactt ccaagtatga gaatttttaa agaagattta
     1141 ggtaaaaaat catctacttt agtatatctt gcaaagcttt catttgttgc tatgattggt
     1201 aattttgtag gattggctac tttaatacca ggatttaccc ctgcaaacta ttatttaaat
     1261 cccaacatag catgtttgtt agtagggttg atttttacac gaataggttt tttagaaaaa
     1321 ggaattttag ataaatctca atctaatgga attataatgt ttggttgtat gttgatgtta
     1381 cctggaggac ttgctaaggt aactccaaca gcattgtttg ggatgattat tccaactata
     1441 ggaatacttg ttgtaggctc tgtatttata atactattta ctgcaattgt aggaaaacta
     1501 ttgggatatt ctccaagaat gtcagctgca attggtgtaa cttgtatgct agcttatcct
     1561 gcaacacaga taatttccaa tgaaggtatt gatgctatgg aaggaactga agaagaaaaa
     1621 caaagggcaa cagattattt attaccaaag atgattatag gaggatttgt tactgtaacc
     1681 attgcatcag tggcatttgc tagtataata ggtccaatta tatttggttg atagtaaata
     1741 gatttatagg aggaatagaa aatgatagga agtatatcta taaatgagat agataaaaaa
     1801 agattcaaac ttgatgattt aagtaagaaa atatgggaaa atccagaaaa agcatttaaa
     1861 gaatttaaag catgtgaaaa tacagcaaac tttctaagaa gtgaaggatt tgatgtggaa
     1921 gtaggtgtag gaggtttagc cacggctatt agagctagtt ttggttcagg taaaccagtt
     1981 ataggtttta tgggagaatt tgatgcatta cctggtttaa atcaaaaagt ttcaacaaaa
     2041 caagaagcat ttgaattggg tgcatatggt catggatgtg ggcataattt attatgtaca
     2101 gcacatgtag gggcagtagt agggcttaaa agagagatga tagaaaataa cttaagtggg
     2161 actatagtat tttatgcatg tccaggagaa gaacaattaa caggtaaagg atttatggct
     2221 agaggtggag cgtttgaagg gttagactta gctataaatt ttcatccaaa taaaattagt
     2281 gaagctacaa ttggtacttc aacagctgtt aactctgtaa agtttcactt taagggaaag
     2341 actgcacatg caggctcaga ccctcaaaat ggaagaagtg cactagatgc tgtggaactt
     2401 actaatgtgg gagctaatta tttgagagaa catgttactt ctgatgtaag gattcattat
     2461 acaattacag atggaggagt agcacccaac atagttcctg ataaagcatc agtatggtat
     2521 tatacaagag ctttaagtag agaagctgta gaaagtacat atgaaagact tgtaaaggta
     2581 gcaaaaggag ctgcaatgat gactgaaact gaagttgaag tagagttttt aggagggtgt
     2641 tataatactt taaacaatca tgttttagca aatcttgtat cagaatgtat gaatgagatt
     2701 aaacaagagc cttggactcg agaggaattg gattttgcag ctgaattaga tagacaaagc
     2761 ccacaacaat ataaggctat gataagtaaa tataatttgc cagaaggaac tcatctatat
     2821 tatggtggag gaaatgttac aaactttaat agttatggct ctactgatat tggagatgta
     2881 atgcatatag ttccgacagc atactttttt acagggtgta cgaatctagg agcaccaggt
     2941 catagctggc agtttacatc ttgtgcagga agttctatag gagaaaaagg tatgatatat
     3001 gcatcaaaga ttatggctat gtttggagct aaaatattaa ataatccaga gatagctaaa
     3061 aaggcaaaag aagaatttga taaatctatg aatggtaaaa catataaatg tcctattcca
     3121 gatgatattc caactcctta aaaagacatg aaatttaata tataaaatta taatttttat
     3181 atcagcatca tttcattact aaaagaaagt gagattttgc tgactatctg attaggagga
     3241 aggaatatga aaaatttttt taaagaagcg cagtcaatag aaggggaaat tattagatgg
     3301 agaagatatt tacatgaaaa tcccgaaatt ggttgcaatc taccgaaaac agtaaccttt
     3361 gttaccagta ctttagaaaa aatgggatat tctccgaaat tgacgtctaa taatggaata
     3421 gtagctgttt tagacagtaa aaatggtgga aagaccttat tattaagggc agatatggat
     3481 gcacttccta ttatggagga gacggattta ccttttaaaa gtaagaataa agatgcatct
     3541 catagttgtg gacacgataa tcacacagca atgctcttag gagcagctaa aatacttatg
     3601 gataataagc acttacttaa aggtagagta aagtttgtat ttgaaccaga tgaagagact
     3661 accagaggtg caaaatcaat gattgatgat ggtatattag aaaatcctca ggtcgatgca
     3721 gctatggcat ttcacagtat ggtgggtaaa tttcttaaca caggtcaagt agcttattca
     3781 cgtggtccag ctatggcatc tgcagatata tttgaaataa cagtagaagg aaaagcaact
     3841 catggagcaa gccctgaatt tggagtggac ccaataaaca taatgtcaca tatacaaata
     3901 gcattaaaca ctatactttc aagagaaaaa cctcagaaag agcctgtagt tttaactatt
     3961 ggtatgttta atgcaggaag cgtaccaaat gctataccaa ataaagcatc tatgtcaggt
     4021 actttaagaa catttgacca atctgttaga gaaaaattaa aaaatagaat tattgaaata
     4081 tctacaggaa tagctaaatc atttggagga aatgctaaag tggaatttaa aatggggact
     4141 cctgcagtta taaatgattt ggatgtagga gatgagattg tatcttatgt taaagaagtt
     4201 ataggaaatg aaaatgtaat agactttcca tctgcaatgg gttcagaaga tttttcagag
     4261 attttactaa gggttccagg agtattttta tggattggaa tgggaagtat tagtcaggga
     4321 tataattttg gtatgcataa tcctaaagca atttttaatg aagagggatt agtttatggt
     4381 tcagctttat ttgctcattg tgcagtaaaa tggttagaaa ataattttag tagataataa
     4441 cataaaatat tattaaatat tataaaaggt atatcttaaa aagatgcaaa gatatacctt
     4501 ttagaaagtc atatataatt ttacttaata ttagatgata aaatattttt atgcattttg
     4561 gattttaata tttattttct gatataaata atttgtatac tctttcaggt ctaccttttg
     4621 ttgagttttg tttttcatat aatatagtag cgtttccaga ctttaagaga ctacttagaa
     4681 atctattagc agttctaact gttacattta aacatgaaga taattcctta ggagtaactt
     4741 catctctttg tagaatttca gtaacagata taatcttttg aatagtaaga gtagacaatt
     4801 tagaactttt ggcaattgta tttatatgtg gagttacttt atttgaaaca gtaagtaact
     4861 tatcacacag taaaggtcct attgtctcat aattttcgtt gactaagaaa ctgtaattta
     4921 aatgatttat tttagattcc ttattagcac ttatggcact agacatagct tgctttatat
     4981 cattaccaat tccatatcca acacatactt taatatttgt actattttca atagattctt
     5041 ttatataaca tgcagtaaaa tttttagtta atgatttaat cgtttcacat ttagtgaaaa
     5101 ttttaaaaga attactattt ttttcaatta taaaatcggt taatttatct tttttaatac
     5161 tatttaatat tttataaact tggtctagag tagagtcatc ataatttgta tctttaactg
     5221 ttatatatat tacacttggt tgatttccct tcattctatg taaatgaata tcttttatta
     5281 agttattaaa agtatttttt aataaatcta tatcagggta tatgaaatga aagtttactc
     5341 ctgcattttg taacatagga acaatactac taaaacgagt agtagaaaaa tcgattttcc
     5401 cttcattcca tagattaata tgtttttttg caatcttttt ttcaagtttt gctatgtcag
     5461 ataaactcat tttagatgca ttttcatcaa tactatccat taagtctcca aatgtgccat
     5521 ttgataaata atatgatata tcttttggat tatctgccca gtctgttaag tcaaagtacg
     5581 ctctagaaaa atctaatgta ttatatttaa agataagctt tagaaacata tcatagtaac
     5641 tttgtaagtc agtaccaaaa tcaactagag gttttttaat agttccagcc ttttttgtaa
     5701 tagctttttt ggggatgggg ccactaactg caaatccatc aaacctatct tctatttcta
     5761 aatataaatc tccaacatga gaaaaatctc tatagatata tatttcaatt tctgcattaa
     5821 tatttaactt ttttatagaa ttatctacaa actcttttaa aaattcggtt gttatgactg
     5881 ctattttcat catagattac ttccttttca attattataa ttattttata ttaaatatta
     5941 cattaatcta ttgttctagt taaaatcgta tttcaattaa attcaattaa aataaataac
     6001 tctttataaa aagcaattat ttaggtagag gaatatttgc taactttaat agtctttgtt
     6061 aactttaatt ttatttaagt tattatagtc ttactaattt tataattgta gacttaacta
     6121 ctttatgttc ttttattgtt tatagtagtt gatttagaag ttattttaac catttatttt
     6181 aattgaagtt gtttgatttt taaatccttt gtgtaatctt tgtttttcta gccatgataa
     6241 aagtatttct atttgtatca ctatatataa taatatcatg acaaaatatt tattgtctaa
     6301 ctaattataa tctatccaaa atgaaaatgg gtgtgagata ttattacaac aaatcgtatt
     6361 agtattaaaa aagattaaaa aattaaaaaa ctaaacagtt aaataattaa acgattaagt
     6421 gactgtcata taacaattta taattttatt taacagccac ttaggtattt aagagcgata
     6481 gaattgagtg tatatcaaat tataataatt tcctagtaaa taattgaaaa ataaggtagt
     6541 ccatttttat atttgatatc tggtttacct tgaataagtg gatatagata atttagggct
     6601 tcatcagtta tataattaca ttcttcattt atccactctt taggaaaata ttttgtcttg
     6661 ttagctattt tagaaatatc agtatgacta acttcatact tataaggatt attgcatgtt
     6721 ctattaatga tagccataac tcctgtatat ccttttttag atagttctat cccatatgca
     6781 ccaacttcag ctgcttgttt taaatcagta tctgaagcta aatgcatagc acatctttgt
     6841 attacaccaa gttctaaggt ttttactctt gttgtaattg atgatgatac tataagattc
     6901 tttagatagt tgcttactcc acctaattgg gaatgaccaa atttatcatg agcagaagtt
     6961 ttttgttcgc atataaagtt gccgtattta tcccttatac cttctgacgc aactatataa
     7021 gccttatttt tttctttaaa tatttttcta acatcttcta taaacttatc ttcatagaaa
     7081 tcaatttctg gaaggtatat taaatcaaca agtggagtat cattaagtct agctagtgaa
     7141 gaagaagctg ctaaccatcc agtatctcta cccatagttt ctactataaa tataccatta
     7201 tttttgtata tagaaaaatc taagtatgtt tccattatta gtgtagaaat aagctttgca
     7261 gcagaaccaa atccaggaca gttgtcagtt cctaataaat cattatctat tgttttagga
     7321 atacctataa attttatttt acaagtagtg tttttgcagt attgagaaaa tttcatcact
     7381 gtgtccatag aatcatttcc gcctatataa aataatgtat ctatttcata ttcatctagt
     7441 atatttataa ctctatcata atcataagaa tttattttat aatcagccag tttgtatcta
     7501 caagaaccaa gaccagacga aggtgtttgc tttaaattta atacattatc tttgtttaag
     7561 gagtctgaat ctattatatt tttgtttaat ataccttcaa tcccattgat accaaaatat
     7621 actttatcaa agtatttagt ttctaaattt ttttcataaa taccaactgc agaagcattg
     7681 ataacagctg tagggcctcc agattgggca ataatacagt ttctcatttt acatctccct
     7741 ttcattaata ttgttatgaa tggtactttt tatcgagtaa atttatattt atagtctgat
     7801 tatatattaa atgaactagc tatatcaatt gagttagtat agacaataaa tgccataaga
     7861 atgtggtaaa aaactaactt tttatataca attaatgtct ataatttacc acaatataaa
     7921 ggtactttta aggagtagtc aaagtgttgt gaaaaaaaat atatcagtgt aatattaatt
     7981 tacaaagcaa aataagttca cagttaacaa aagcagaata agttcataaa agaaatatat
     8041 acagcaataa aagtaatgct aaataaaagt aatactggat taaaaaatat taaaaaaata
     8101 tcaaaaacta atatttaata ctaattaaag taaaatatgg aggtatacaa atatgaaaca
     8161 cataaaaata gcagcaggat tagcacatgt aaattacggt catatatcag ccgtagttaa
     8221 agaagcaatg gatgcaggtg tagactacgt gcattcagat gcagcagata tgcatgactt
     8281 aaaaaatatg caacttatgg gtggtcatca aataatagaa gcaataagac cagtgacaga
     8341 taaaccaata gaatgccata tatacacaag agattgtgat agattattta tagaaaaaat
     8401 agcagcagca ggatgtaact tgttgataat accagcagaa cattttttag gtgctcctct
     8461 tgcatacata ataaattact gtagagaatt tggtatgaaa gtaggattaa caattggatg
     8521 ttacacacca ttatgttttg ttgatgaagc tatatatgat atagatagat tgcaaatagt
     8581 cgttcatggt gttgatgaaa ctgatggaaa agataactgg ggatggagaa agagtgctgt
     8641 tgatttagta aaaagagcta gaaaaatgat agacgaaaag aatcctaagt gtgaattagc
     8701 aatagatggt ggactaagag cagacaatat ggagccatta atagaatgta atccagacgt
     8761 tgtaatatta tcttcagcaa tattcaagga taaagatggt ataacagcag gtgttaagaa
     8821 ttgtagaaaa gctatagata aagcagctac aaagtttggt ttagaataag ataataaact
     8881 atgataaaag agatgctttc ttaatgtttt aaggaagcat taattttatg aaaaactaca
     8941 ggaagctaga ctaatttaat tgatttttta tactgaaata atataatcat aggaggttat
     9001 taaatgaaaa aagaaatgat aaaagattat ttactgataa caatatcagc atttttaatg
     9061 gcattagcag ttaatttttt ctttgcagaa catacattag ctccaggagg gataacagga
     9121 ttatcagtcg tacttagcac attcttatca atgcctgttg agaatatatc attaggaata
     9181 tctatgccac ttttagtaat gggattatta tttttaggga aaggttttgg aataaaaaca
     9241 ttatatataa ctcttatggg cccattattc ttaaaagtaa taccacaaac tcacataact
     9301 gacaatctat tagtagcagg agtgataggt gggttattgg taggaactgc tatagggata
     9361 gcaataataa ggggctgtag tactggtgga actgatttag ttgcaatgct tattaataag
     9421 gtaattaagt ttctgaagtt gccagttata ttatttatgc tagatggatt tataatagta
     9481 gcgtctggaa taataagtaa gaattttatg atatctatat attcattgat atctttatta
     9541 ctcataataa aaacaatagg atttgtaact acaaagtttg gtaagaatac tgaagaaatt
     9601 aataatataa ataatgcttg tgctcaataa aaataataaa atttacttta aatataaaag
     9661 tttaaaaaat ataaaagctt aaataaaaag cttaaatata aaaaatggag gtgtctcaaa
     9721 atgaattttt tagttcatga ggcacttttt ctatgaaatc aaatatattt ttatcattta
     9781 gacaatgaaa aataggttta tctctatgtt gagacaagcc tatttttgtt tatttataag
     9841 ataatatttt atttttttca gaatatagtt tttttataat tgtactaagt ctagaaatat
     9901 cattcttact taaaagagca tttatattta tcagttcagc attatgttca aagtctacag
     9961 gagcagtggt tagtattaag tccacatcat caaagttcgt aaatgaaata gaagatagag
    10021 gtatagattt aactatatta atctgattaa attcaaaact taacttttgg actaataaat
    10081 tagatacacc atcactacag tgacatatta aaatagtatt aaggggagat ttgcttctat
    10141 ctattgctga tattaaatat aaagcaagat atcctatttc gtcatcattg acttcataat
    10201 tcatcaactg tttgataaca gtattagact ctttagcagc cttataagta taaggaaatg
    10261 ttctctttat atcatttaat atgggatttg taatagtaaa cccatagttt attctgtgta
    10321 gaactgattt aaggtgatta gcaagagact ttattaatat taaatcatcc tttaagttta
    10381 aatttagtat acttccccaa tgaacagcta ttgcttcaga tagattatac tctgtaattt
    10441 cttcattttt aagaggttca agagtagtag ttttctttat tgatttaagt aatccttcta
    10501 tatgaagaag gatataacag acctcttcat caataaattc tacgtcaaaa gcttcactta
    10561 ggttagcaca taaaattttt gatttttcaa agtattcatg ttctaataat tctctaaaaa
    10621 aaccttcaga taagttaata tatcttttaa ttgaaattct tttaatacat atagctatat
    10681 gtattaagaa gtttatataa tactcatcac taaggttact tttttctact tgtaaggtgt
    10741 gtagagtgat ttgagataat ttctcaaaat catagttaaa tagctcctta aatatttttg
    10801 ttgaaggggt atctttgcat tcattagatt tcgagaataa tatttcttta agttcagtat
    10861 aacttttatt aaccgtagca agttcaaaaa ttgcttttcg aatatctttc tcttttccct
    10921 ctaaatatac tccattattg gtttttctaa ctagagtcac tttaaagttt tgcaaaaaat
    10981 tttgaataac taccaagtct ttatgcacag tagctctact aacatgtagt tcattgcaaa
    11041 tatcacgaat cctaaactta gaatttttaa taaataattt acttagaata tacctttttc
    11101 tatcttcagg agagtatgca tatatacaat ttgatttatc attgatatct ctaataactt
    11161 taagttttat atcttcatta cctagaataa taacccctga accagttttt ttatctaggg
    11221 ataaattaaa ctctaaaagc cattcatcta aaataacaag gtcattacga atagttttat
    11281 tactaacttt taattcttcg gcaatagtat ctacagtaat atagtctgag ctgttgagta
    11341 ataattcagc aatatctaat aatcttttgt tcaatgcaaa ccctcctaaa tcttagttac
    11401 tatttattaa ccttggacca atctctttca aaagtattgg caccatctat tgtaagctgg
    11461 ttaaaagaag cttttttaaa taaatcatat gttacagctg cataatcaca tccacaaaga
    11521 gatgcttgag ttaagtggaa tacattttta atagaagctg caacaacttt agtattatat
    11581 ccatttattg atataaggtt acatatttct gtaaggtttt ttataccatc attcccattt
    11641 tcatcatttc tacctataaa agggaatagg taatcagcac ctgcatttat agctaatgat
    11701 gcttgattta cattgaaaat caaggtacat gcagttttta cacctttact atttaatatg
    11761 tttataagtt ctaagccaac ttcagagaaa tttattttta ttatagtgtt tttatttata
    11821 tttaatatct tatcacatga attaattaat tcgtataaat tatttccaat gacttcagca
    11881 gttaatattt ctggatttaa attagcacat gtctcaataa attcatggaa gtttactttc
    11941 tctttaagat acatagatgg attagctgtt attcccttaa atccatactt taaacacttt
    12001 tgaatttcat caagattagc actgtctatc aaatatttca taatttaatt ctcctttaag
    12061 ctatttgtca taaaaaagtg tatatactct atttttatta ttaagtcaat tatacaataa
    12121 gattgattat aaaattataa catgggtact taataaaatt tacataatga tatggtaata
    12181 ataaaacatt gattgtctaa taattgccat aagctttagt aaataagagt taacagtgaa
    12241 ggtattgcct taaaaatcct atgatatgta taatttgaat atgaaggcaa taatttaagg
    12301 gaggaaaaat atgattaact tttatcaacc atcaagaata aattttggac aaggaacttt
    12361 aagtgaatta cctagaataa taggaaaata tggaaagaaa tgtttgttag ttactactcc
    12421 aaatgtagag ccattagaca agttatataa aagagttaca ttgaatttaa tagaaaaagg
    12481 tatagaagtt gtacactttg atagagtaga gccaaaccca actgtagaat taataaatga
    12541 agggtttgaa ttagctaaaa aagaatctgt agatgttgtt ttagctgttg gaggaggttc
    12601 aagtatagat actgcaaaag tattagcatt gacttttggt ttagaaaaga tagattggga
    12661 ttatatgttt agtacatata caaatccatt taaaatatac gataaggtaa gtgaaaaaga
    12721 attaccatta atagctgtat caactacttc tggaacagga agccaagtta cacaagcagc
    12781 agtaatatcg agaggaagcg agaaaaatac aatatttcat caaaattgtt tctcaaaaga
    12841 atgtatagtt gacccagagc ttatgttaac tctgccagag agaattactg catcaacagc
    12901 atttgatgct tttactcatg catttgaaag ttatataaat ccaaatgcta atgattttac
    12961 agagataatg tctgaaaaat ctatggaatt agtcattaac tatctaccta aagtaatgga
    13021 agataaaaat aatatagaat acagaacaaa gttagcagta gcagatacat taggaggaag
    13081 ttcactggca aatgctggag cagcagcacc acatccacta agtgaaataa taggaggagt
    13141 tacacatatt cctcatggtg aggctttggc tatagtgttc ccaaaatttg cagaaaagtt
    13201 ctataaggat aatatagaga aatttgccaa agtagcgaga atgctaaacc aagaattaga
    13261 aaaagtggaa gatgatgaag tagctaaaac tttaggaaat gaaatagaaa gttttttaaa
    13321 gagagtggga cttaagacaa aaatgtctga gtttaatgta acaaaggaac aattagaaca
    13381 aataacaagc tgtccagttt taggattttt accttttgga aagaaagaag accttgttga
    13441 aatattagaa aaatcttact aggatgtgat acaaatgcga tataaattaa tttgtactga
    13501 tatggatggt actttgatgg gtaaaggatt tgaagtatca gaagaaaata ttaaagcact
    13561 aaaagaagtc atggaaaagg gtatcaaggt agccttagtt acgggaagac cttataatgc
    13621 tatgaaatat tttacaagtg ttttagggga tgatatatat attatatcaa ctaatgggac
    13681 atattttaaa ttactgggat atgagtataa gaaggttttg agcaaagaag ctctaaagaa
    13741 aatctataca ataggtgaaa agtataattt aagcaaacat tttaaaggct gtaaaatagt
    13801 tatatccaac aatgaaatag gtgaagaaca tccatataga ttaataaata gtaaaaataa
    13861 agaagaagac agaattgaaa taatagaaaa tgcaagttgt gaaactttat tagaaaaagc
    13921 tgataatgaa attttaaaat gtatactttt ctcagaaaac gtagattctt tgagagaagc
    13981 aaaggaagaa tttaaaaaac aagaagattt agaagtagta agttctggaa agataaactt
    14041 tgaagttatg agtaaaggta cttcaaaagg tattgcagtt aaaaaatttt gtgatatatt
    14101 aggtatagat agcaaagaag ttatttgtat aggtgataat gaaaatgata tatccatgat
    14161 taaatttgca ggtcttggta ttgctatggg aaatgctaca gatgaagtaa aatctatggc
    14221 agattttgta actgatacta atgtaaatga tggtgtagca aaagctttaa gaaaaatatt
    14281 atcataaaat tttataatgt ggagagtgtg ttatgtttaa tatatttaag aaaaaagaaa
    14341 ataagattga gaaaatatat agccctttta agggaaattt agtgaactta gaagatgttc
    14401 cagatgcagt ttttgcccaa aagatgatgg gagatggagt agcaataata ccaagtgaag
    14461 gtaagttata ttctcctgtt gatggtgaaa tagtcaatgt atttgataca aagcatgcaa
    14521 taatgattaa gagtaatgat aatacaaata tattaataca tgtaggttta gagacaatgt
    14581 cactagaagg aaagccgttt gatgttaagg taaaggcagg agataaggta aaagctaaag
    14641 atttaataat ggaagtagat ttagatttta taacgtcaaa tgatttaaat ataataactc
    14701 ctgtagttat aatggatgat gctgattctg ttaagagaac agtaaaagat aggatagaac
    14761 ctggaaatgt agatattaat agcataataa tggaagttga atagatataa gaaggtgtag
    14821 ctaaggctac atcttttttt atgtattttt ggaataaact tacattaaaa aagacgtaat
    14881 agataagtta aaatattagt aataggtata gtatgatttt gtcataatct gttgattgtt
    14941 taccgtatca ttatggtaac ttagagaagt atttgaaata ttgaaaaaat aatattcaaa
    15001 atttataatg tagatatgaa ctgaaatgct ttgtaaaatt aatataaagg ggtgataaat
    15061 aattaagatg atagtaaaca aagaactaat tattgattat ttaaaatcca atcaagaaaa
    15121 tatcataaat gatattagaa gtttagttga aataccttca attagagatg aaagtacaac
    15181 agatataaat caaccatttg ggatagaaat aagaaatgcc ttcgataagc ttattcaaat
    15241 agcaaaagac aaagattttg ttgtaaaaga ctttgatgga tatgcaattc atatagaata
    15301 tggagaagga gaagaagtag taggggtgct taatcatata gatgtggtgc caatatataa
    15361 taaagaactt tggaagagta aaccttttaa agtatgtcaa aaagataact atctttatgg
    15421 tagaggagtt aatgacaaca aaggaccttt gatagggatt ttatatgcat tacttttctt
    15481 aagagaactt aatgaaaagc cgaagagaaa gattagattg atagtgggtg gagctgaaga
    15541 gacgacttgg gaatgtatgg agcattactt tagtgtcaat gaacagccaa aatttgcatt
    15601 ttctccagat ggaaacttcc caatagtaaa tggagaaaaa ggtattttat acttcaatct
    15661 aagaaagaaa atagataaag ataaatttag aaatcacaat ttagttgata taaagtcaaa
    15721 caaagaagat ggatttgtat gtgacaaaat tgaggctgtt tttaaaacta atgataagaa
    15781 agatttagta gaatcattag cttattatac agagatagaa gaactagaag aaggtaaagt
    15841 attagtaaga tatacaggag aaagggcttt atcgagaaat ccacacaggt catataactg
    15901 tgcatttagt ttagcaaaag atttagagaa aattaaaaaa ctaaatgata aaggcgttat
    15961 aataagggat attttaaatt catactttac agatgataat catggaaaaa aattagggat
    16021 atataaagaa gatgtagata tgggagtttc tacaatatgt ataatgagta tcttcttaga
    16081 gaaaaatgaa ttgaatatga aaatagactt tagatatcct aagggtataa gttgggagtt
    16141 tataactaat agaattaatg aaattggaaa aaaagaaaat ttaatagttg atatatataa
    16201 ggatttaaaa ttactgtatg taaaaccaga tagtgaatta ataaataaat tatcaaatgc
    16261 atataaacaa ggttttggaa aagaagccga actttttaca aaaggagctg catcttatgc
    16321 aagagtgtta aaaaatggag ttgcatttgg acccacaata gagggggata atccaaattc
    16381 acatcaggca aatgaaaata taagtataga tacattatac aaagcaatag aagtatatat
    16441 atatgcatta tatagcctgg catttcaata aaaatggagg aaataatact atgaaggata
    16501 aattacaagt actttctgga gcaatgatga tacctgttat attacttgtt gtagcaggag
    16561 tatttatagg actagggtca gcttttgcaa atatggaaaa cgtttctgca ttagggctta
    16621 gcggactaat aaaagaaggg tcatttataa atgtattctt taaagtaata aacgatttag
    16681 gatttatggt aatgagatac ttaccaatat tctttgttac aggtatagcc tttggattat
    16741 ctaaaaagga aaaaggatgg ggtgcattag gaggaattgt tttatttata ggaatgcaca
    16801 cagttataag tacattgtta gcagcaaatg gtataaattc agatacagtt acagcagaag
    16861 cgtttatggc atctggatta ggagaggcag aagcatatag tcgaagttct ttatatggtt
    16921 ctttcttagg aatcttttca tatgattgta gtatatttgg agctataata gctggatttg
    16981 tagctagtgc agtacataac aaatttgttg acatagaact tcctagtgca ttatcttttt
    17041 tctcaggtcc aagattttca atgattatga tgtttatagc atcaatagta ttgggtacag
    17101 caatgtactt tatatggcca ccagtagggt tggcattatc taaacttggt aattggatag
    17161 gttctagtgg attattagga acatttactt ttggagcaat ggataaagca ttattaccat
    17221 ttggtataca tcacttaatt gcattcccta tagaatatac aagagtaggt ggaacaatgg
    17281 aagtaggtgg agcaatttac gagggtgtta ataatataag attagctcaa atgggagacc
    17341 cagatacact atcttatata actcgtaact tcacaacagg tcgtttatta atacattttg
    17401 caatattacc agcagcagct ttagctatgt ataagttggc tgataatgct aataagaaaa
    17461 aagcaataag tatattgata ccagcaatag taacagccat gttggtaggg gtaacggaac
    17521 caatagagta tacattccta tttgttgcac cattattgta ttttgtagca tatgtaccac
    17581 tggcaggatt gacgtatgta tttacagaaa tgtttaatgt ttctataatg ggagaatcat
    17641 ttagaaatat gtttcctaat ttattacaac cacaaaaagt ggatgcgtta ccattattat
    17701 ttttgatacc aatatttttt atagttactt atataatatt tacatgggca atcaagaagt
    17761 ttgatataaa aacaccagga agaagttcag aagaagttaa gctttatagt aagaaagaat
    17821 atagagaaag acaatctgta gatgctggta aaacagcata caacaatgca gtagaagagg
    17881 aagtagatga taaagataca aagcttgtac attctataat agaaggttta ggaggaagta
    17941 ataatataaa aaatgttaca aactgtgcaa ctagacttag ggtagagcta aattcaattg
    18001 atggattcta tgaagatggc ttctgggtaa atgaattggg tgcaagtgga gttgttaaaa
    18061 agaaaaactc agttcaagtt atatttggtc cacgagttat tacaatagca tcaaagctta
    18121 aagctgtact tggagtagat taatgtatat aaagctgtgt aaattgataa gtttacatag
    18181 cttttatttt ttattataaa ggtattataa taatttaaaa tttgtggaaa gtttgtgtgt
    18241 atttaaaata tactacagta tttaaattat atggagaaaa aattatttgt gaaaaatgga
    18301 atgaaatcat aaagtaagaa ttattatact gaaaatattt acaatctcga catattatgc
    18361 tacaattact ttgaattaat aaaaattaat aagaattacc tcatctaaca aacagattga
    18421 ggtagaggtt gcatttacta agattaatat tgaagaggtt ggaaaccaat gaggcaatat
    18481 aaaaggagta agtgccgaag tgctagtgtc cgaagctagt gctgggactg tatttaatag
    18541 gtacagtact gtcatcaaaa ttatcccgaa ttttgatgga gagctatctt aaacaagata
    18601 aagggaagat gcaggtatgt tgtatttagc acatttagct caacatgtat ccatgttgag
    18661 cttttttaca tctatacaaa ataggataaa gggatgcaat aataaaatat ttaatttaaa
    18721 aagtggggag attaaaaatg agcagtttaa aaaagaaaaa gtttactatg ccacagacac
    18781 ttacaataat atttttgcta atagtgttaa tggcaattct tacttggata gtgccaagtg
    18841 gaaattttga aagagtagat atagacggaa ggtcagttgt tgtagctgga acttatgaaa
    18901 aagcaccatc aaatccacaa ggaattactg atgtatttac agcacctata aacgggttta
    18961 tagatgctgc tgaagtagtt ggatttgttt taatagttgg aggagctttt gggatagtta
    19021 ataaaacagg agctatagaa gctgttatag ctcatactgt aaataaaatg aagaagtttc
    19081 aatttttaat aataccgatt tcaatgattc tatttggact aggtgggacg acgtttggaa
    19141 tgagtgaaga gacactacct ttttatatga tatttattcc acttatgacc agtatgggat
    19201 atgattcatt gacagcagtg gccacagtat ttataggagc aacagctgga tttggagcag
    19261 ctactacgaa tcctttctct gtaggaatag cacaagcatt atcacaaata gcaccaggtt
    19321 ctggaataga gtttagagtt gttatgttta taatatatat ggctatatct ataggatttg
    19381 ttatgatgta tgccaataaa gttaaaaaag accctaaaaa atctttagta cacgatatat
    19441 cactaaatca agaatcaatg gttaatagtg atacaaatat aaaggaattt acaaaaagag
    19501 aagccatggt aatagcaatt ttcacaatag gtatggcgat tatgatatat ggagttttaa
    19561 agttgggatg gtatataact gaaattgcaa tgatatttac agctataggt ataatatcag
    19621 gtatagcatc aggtcttaaa caagatgata tagttaattc atttataagt ggagcaggag
    19681 atttaatgac agcaggattg tgtatagcat ttgcacgtgg aattgtaata atagcagaaa
    19741 atggatttat aatagataca attttaaatt ctgcagcaaa tttattaaat ggattaccaa
    19801 aaactatatt tataaattta actttcttaa tagaaggatt aatagcattt ttaataccat
    19861 cagcatcagg gcttgcatca cttacaatac ctgtacttgc accacttggc gacttagtag
    19921 atgtatcaag acaaatgata gttacagctt atcaatttgg tataggtgta actaacttga
    19981 taacacctac atcaggtgtt ttaatgggag cactagcagt agccaatatt ccttggtcaa
    20041 agtgggttag attcgtaatt cctttaatgg tagtattaac aattcttgta atggcatttt
    20101 tgacaatagg attatatcta ggattctagt aggaggtaaa ttatgataga gagtataaag
    20161 aagagagctt atgagataga gaatgaactt ggaaaagaaa ttgaagaagt ttgtaatttt
    20221 atatttaaca atcctgaatt aggggaagaa gaatacatat catcaaaata cttagtagaa
    20281 aaaatgaagg aatatggatt tgatacagtt tatccatatt gtaatatgga gactgctttt
    20341 agagctgaat taggagataa tgatggacct accatagcat ttttagctga gtatgatgca
    20401 cttcctggat atggagaaaa taaagaacca gctcatgctt gtggacataa ctggattgca
    20461 gcatctacac taggagcttg tatagtatta agtaagttaa aagaaaattt taaaggaaaa
    20521 attgtactaa taggaacacc agctgaagaa acaacaggag gaaaatgtga tttagttaag
    20581 gcaggagctt ttgatgatgt tgatgtaagt tatcaaatgc atatagaagc ttttaataat
    20641 ataaactgta aggctttggc gatagattca ttagagttta gttttgaagg ggtatcagca
    20701 catgcagcta gtcaccctca tatgggagtc aatgcactag atgcagtaca acttactttt
    20761 gctgggataa atgcactaag acaacatgta aaatcagatg ttagaataca tggtatagtt
    20821 tcaaatggag gagaggctcc aaatatagtt ccagaaaaag cagcttgtaa gtttttcgta
    20881 agagctgctg aaagaagtta tttagatgaa gtaactaaaa aagttataaa ctgtgcaaaa
    20941 ggtgcagagt taatgacagg agctaagctt tcttatagat actttgaaaa ttcttttgac
    21001 aacataataa ataacaaagt acttcaaaag ataacaaaga ataatttgat tgaggcaggt
    21061 ataacagata tattagaagg taaagatggt ccagtaggtt ctacagatat aggtaatgta
    21121 agtcaagttt gccctactat gtatacagaa atagccttag atataagtcc aatggtatat
    21181 gttcatgaaa aagaatttct aaattatgca aattctgagg aagcttatga taaacttcac
    21241 aaagcagtaa aggcaatggt aggatgtgca ttagaaattt atttagaaga tggactactt
    21301 gatgagatta agaaaaacca cttaaattag aaataaatgg gtattactta aagaaagtaa
    21361 tacctttttt attttgttta ttgataaaaa tagtactttg aagattcaat ttatataatt
    21421 tatttaaaat taaaatattg agaaaaagtt gacactaaaa aataaatttg ctaacatata
    21481 ataaataata attaaaataa aatacaaacg gatagtgatt gtttaaatac aagctaaacc
    21541 tatttattac tcaggactca tgggagtcta tttaatg
//
LOCUS       DAEMGG010000062        21113 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEMGG010000062 DAEMGG010000000
VERSION     DAEMGG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.062"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            536..1567
                     /locus_tag="KPK24_002179"
     CDS             536..1567
                     /locus_tag="KPK24_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG2299851.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1700..2998
                     /locus_tag="KPK24_002180"
     CDS             1700..2998
                     /locus_tag="KPK24_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG2299852.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3112..3357)
                     /locus_tag="KPK24_002181"
     CDS             complement(3112..3357)
                     /locus_tag="KPK24_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG2299853.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3505..4371
                     /locus_tag="KPK24_002182"
     CDS             3505..4371
                     /locus_tag="KPK24_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG2299854.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4375..4911)
                     /locus_tag="KPK24_002183"
     CDS             complement(4375..4911)
                     /locus_tag="KPK24_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG2299855.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(5102..5305)
                     /locus_tag="KPK24_002184"
     CDS             complement(5102..5305)
                     /locus_tag="KPK24_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG2299856.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5596..6360
                     /locus_tag="KPK24_002185"
     CDS             5596..6360
                     /locus_tag="KPK24_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2299857.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6749..8062
                     /gene="prdC"
                     /locus_tag="KPK24_002186"
     CDS             6749..8062
                     /gene="prdC"
                     /locus_tag="KPK24_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG2299858.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8466..8551
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            9133..11382
                     /locus_tag="KPK24_002187"
     CDS             9133..11382
                     /locus_tag="KPK24_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBG2299859.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTSDVEKPSDGQVPDGEKPSDSQVPDVEKPSDGQVPDGEKPSDSQVPDGEKPS
                     DGQVPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQ
                     TPDVEKPSDGQMPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINK
                     IVYNFWEDEEDVKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYH
                     DLRFDDITKTLTFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSP
                     YLPAEDFNGQENQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWI
                     ILGQMDEQERLKQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAG
                     REELKPNNTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLKLPDGVTAYDG
                     DGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKD
                     HSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPT
                     DDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPP
                     VPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSYVFISVIAICGL
                     AYQCKIKRN"
     gene            11789..13549
                     /gene="prdR"
                     /locus_tag="KPK24_002188"
     CDS             11789..13549
                     /gene="prdR"
                     /locus_tag="KPK24_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG2299860.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            13978..15858
                     /gene="prdA"
                     /locus_tag="KPK24_002189"
     CDS             13978..15858
                     /gene="prdA"
                     /locus_tag="KPK24_002189"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG2299861.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            15939..16223
                     /locus_tag="KPK24_002190"
     CDS             15939..16223
                     /locus_tag="KPK24_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299862.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            16261..16986
                     /gene="prdB"
                     /locus_tag="KPK24_002191"
     CDS             16261..16986
                     /gene="prdB"
                     /locus_tag="KPK24_002191"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16711..16713,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG2299863.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            17066..17824
                     /gene="prdD"
                     /locus_tag="KPK24_002192"
     CDS             17066..17824
                     /gene="prdD"
                     /locus_tag="KPK24_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG2299864.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            17839..18306
                     /locus_tag="KPK24_002193"
     CDS             17839..18306
                     /locus_tag="KPK24_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG2299865.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            18324..18806
                     /locus_tag="KPK24_002194"
                     /pseudo
     CDS             18324..18806
                     /locus_tag="KPK24_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
     gene            18826..19833
                     /locus_tag="KPK24_002195"
     CDS             18826..19833
                     /locus_tag="KPK24_002195"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG2299866.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            19953..20834
                     /locus_tag="KPK24_002196"
     CDS             19953..20834
                     /locus_tag="KPK24_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG2299867.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         8423 a         2443 c         3883 g         6364 t
ORIGIN      
        1 ataatattta tattagtttt tatgcatact tggtataagt ttttcatata ttgattaaaa
       61 ttgatgttga tttattattt ttagtatttt ttcacctttt gattcatact ttgtagaatg
      121 gtgtattttc taaaactgat tataagacaa gttcattcaa attagcattt ttatatcata
      181 taaataattg ttccccctaa ataagattta gatgtatagt attaaaatca aaaagtataa
      241 aataaaattg atgcttaatc atagataaat attaataaaa tatacaaggg gattgtctaa
      301 taaaaagata atctcctttt ttacaaataa ttctaaattg tgatataaat ctaaaaattt
      361 tatcttaaca tttaaccatt taatgataat aattgatata ataaatataa attaaatata
      421 tggtgtactt attttatgtt tttagcaaga aatatagcat ttaaaagata aattgtgcaa
      481 cttaaatcaa agtttaagat gaactattaa catagatata ttaaaaactt ttgtaatgga
      541 gaatatatgt gatatgaata ataaagttaa ggttgcagta ttagatacag gaatagacaa
      601 agaacatgat tacttaaaag ataatttagt tggaggaatt gcttttgaat gtatgcatga
      661 ttatatcttt atatcagata aatttgatga tgaagatggt catggaactg cttgtgcatc
      721 aataataaag aaagagtatg aagatgtaga gttatttgta ataaaaattt tgagaaaaga
      781 tggtattaca aatataaaag ttcttgaaga agcattaaga taccttttag acactaacat
      841 aagattaatt aatttaagtt tatcagtcat aggagtagaa agtgttaagg gcttatttga
      901 agtttgttat gaattgttta gaaaaggaaa aataatagta tgttcactcg ctaatgattt
      961 tgacttaagt tatccagcta tgtttaacaa tgttataggt gtaagaggat ttactttaga
     1021 tggagatgat tcattttggt acaacaaaaa atatgatgtt caatgtgtta tggatagcaa
     1081 ttcatatata agttgtgata taaataattc ctatagatta ccaccaaaat gtaatagtta
     1141 tttatcagca aagcttactg gtaaaatagc taagatatta tcagaaaaac caggcataac
     1201 aatatctgat ttaaatgata agcttgaatc attagcaact agaaatcatt gggatagttg
     1261 tgattttgat gagtgtagta gaattgtaaa ctttaaattg gatttgtatg acaaagaaaa
     1321 tgctcttttt atgaaagttg ctgacgttgt tagggattgc ttagatattg aagagaataa
     1381 tgaaaaactc tttaagggta gcctttttaa taaggagata ggcttggttt atgataattg
     1441 ttttaatctg ttgaaaaagc tagagcatag atttgacata aagtttaatt atatggacat
     1501 ctcaaaatat gacttagttt caatatatac actgactgaa ttagtggaaa gatataaaaa
     1561 cttataaata aagatttgta gctaaattta atgtagaagg tattaaatta tatgtattct
     1621 ttgtcggtag attaaaagta aaaaatatac tataattaga tagcataaga gaaaatgcca
     1681 atacaggggg agttagataa tgaaatatga agaagcatta gagtacatat cacaaactaa
     1741 taaattcggt ataagattag gattagaaaa tatcggaaag ttattagaat tattaggaaa
     1801 tccacaagaa actttgaata taatacatgt agctggaact aatggaaaag gttctgtgtg
     1861 ttcttttgtt tctaatatac taagagagtg tggatataaa gttggtttat atacatctcc
     1921 atatttagaa acttttactg agcgtataag agtaaatgga caaaatatac cacaggatga
     1981 tgttgctaga ataattgagt taataaaaga aaaaatagag attatggtaa aagaaggcta
     2041 tgcatatcca acagaatttg aagtggttac tgcaatggca ttttattact attcagaaca
     2101 aaaggtagac tttgtggcat tagaagttgg tttaggtggc agatatgatg ctacaaatat
     2161 aataacaaaa tcacttgtaa gtgtgatagt atctataagt ttggaccata caggtatact
     2221 tggagataca atagaaaaaa tagcgtacga aaaggctgga ataataaagg aaaatggagt
     2281 agtactagta tatgaccaga ctgatgaagc aaaggatgtt ataaagtcag tttgtaaaga
     2341 gaaaaaagct aaatatatag aagttgattt tgatgatata aacataaaaa aatcagatat
     2401 aaattctcaa atatacgatt gtactgtaat gaaagaaact tatagagacc ttgaaataaa
     2461 attgataggc gaacatcaaa taaacaattc tatcttggca attagtgtaa taaaatatct
     2521 aaaagacata aataaattag caaatataaa tgaagaatct ataagaaaag gacttataaa
     2581 tactaaatgg ccaggaagaa tagaaaagat aaaagaaaat ccaattttta taatagatgg
     2641 tgcacataat gaagatggag caaaatcttt ggcaaaagca cttgataaga attttaaagg
     2701 tagaaaatta accttattaa taggtatgtt agaagataaa gatattgata gtgttttaga
     2761 gatattgttg ccacatttta ataaggtaat aacaacaaca ccaagtaacc caagagccat
     2821 aaattcagat atattaagag aaaaagtatt aaaatatgta gatgatgtaa catcaaagca
     2881 tgagatagag gatgcagtaa attatacatt agaaacatca agtgaggatg atattataat
     2941 aagtgcaggt tcattatata tgattggtac agtgagaact ttagtaaaga aattataatt
     3001 ttataaaata agtatacaaa taaaaatata aaaaagagtt gtctaaaaaa tggacaactc
     3061 tttttatata aaataagttt ttaataattt tactgatgtt tatataagta attatataga
     3121 ttgacgtata gctttagata attggtctgg gcaagaagta ccttttccac cacagtcaat
     3181 accatttaat ttatcagcaa cttcaagagc attttggcca attattaagc ttctaagtcc
     3241 tgataaatta ccattacatc ctcctataaa ttcagcatca acaatcacat tattttcatc
     3301 aacttcaaaa atcatttctc tacaacatac accacttgga ttaaatgtta ctttcattaa
     3361 aaagacctcc attaaatatt atttcttttt ttaacattaa taataatacc aaatatatgc
     3421 gtaataatgc aactataaag cataatacaa gctaatgaaa taatatatta ttgtagtata
     3481 tgtattgtag agaggagggg ttatatgaaa atctatattt ctaactactt atcaaagagt
     3541 attagcatag ttgattattc tacacttgaa ctagaaaaag aaatagtatt agaagataat
     3601 atatatccac accatttttg catagaaaaa gaaaaaaatt taatatacat acctagttca
     3661 agtaatggga tactctatgt cttagattta agtaatgata aaataattga tactgtctct
     3721 ataggaggaa gtcttagtca agttgtactt agcgataatg aattgtttgt ggcaaatgaa
     3781 gattctaata gtatatatgt actagataaa aacacattaa atccaatagg aattattgga
     3841 gttgataata tgccacatgg atttgacttt gatagagaaa gcaaaaagtt atatgtaccg
     3901 tgtataaact cgatagtatg tatagataca attaataaaa atatacaaaa gaaaataaat
     3961 atggacttta gagcttggca tatagaactt gataaacaaa agaaagaaat gtatatatca
     4021 acacttgatg gaaagcttat aataatagat gaagtaagca tggatataaa aaaggtatta
     4081 tatgaatttt tattaccagt agaaataaga tttaattata gtggcaaaaa agtttatgtt
     4141 gctgatttgg gttataataa tgtaagaatt ttagattata caacgagtag atacattgga
     4201 aatattgaaa taaatgggat tccccaagga ttagaaatat ctaaagatga aaagttacta
     4261 tttgtatcag atacacaaga aaattcagta aaagtttatg aaactgcaaa taacaaatta
     4321 ataaaagaaa tcaaggtagg aaaagagcct acaactataa tttgtttgta ggctttaatg
     4381 tatgtctctt ataagtaaat cgataacttc ggcatacata taagatgcat tatttttcca
     4441 gttattgcaa ataagttttg cttgtttctc tgatgaaaca tttagattta aatctattaa
     4501 gttagactga ttttctatga ctctaaggtt gacaataaat tcattatcac tttgttttac
     4561 aaaactagac tttacttgag tatctgctag tagattttct ttgttagaat ctatatactt
     4621 gtctattttt tcagtaacac tagtaggtat tcgattaaag aagtattcta gactttctac
     4681 gcctctttga gtaagagcat aatattctct gtcagagtct ttatatgtag tcaaaaactt
     4741 agattccata agctgagata aaagctgttg taatgaaaaa taattcatca tctcagtctc
     4801 taatactact tgagttattt gagaattagt taaatccatt ttaatttttt ctaatatata
     4861 taaaattaaa agtttgtgat aggctaattc ttcagacgag ttttcaaaca taaatatcat
     4921 cctccgtcaa actatttcta tatataatat attaaactaa tttgtacaaa attaaaagaa
     4981 caatttgtat tgtattatta atctttaata attcatataa aaacaatttt ataaataaat
     5041 atagaaaaac agggctgcta attagcagcc ctgttgatac ccaggtgtca aaatttacta
     5101 tttattttcc agccatttgt ctttcagcca tttcaactaa tttcttagtc atatatccac
     5161 caacatatcc attttctcta gctgttaagt tacctttgtc tatgttttca tagttagata
     5221 aacctatttc gttagctatt tctaatttca tttggtttaa agcagctttt gcttctggaa
     5281 ctactgttct gtttgaattt gacatagtaa atttcctcct caaataatta gtttatgatg
     5341 tctaacaaca cttttattta tgtgatgtta atactaattt ctccaaaata aaaataatat
     5401 atacagaaaa ataaaggtaa aaatgtaaaa aatataaaaa aattaaataa atgagtattt
     5461 tagagagttt aattaggtca atataggttt tattttttta gttattgtct tataaattaa
     5521 atattacagt acttttttga gctgaaaaaa aataaatttt aaatatagaa gaaattattt
     5581 caaaggggga atagtatgaa aagagaagtt aaaaggacta aagctttgat atatagtgtt
     5641 ttattttttt cattattatt agtgttaata tttgtttttg tttatggaat aaaatacata
     5701 tgttctaaat ctgacaattc aaaaatgcct atatataaag ttgatacaaa tgagaaaaaa
     5761 atagctttga gttttgatgt agcatgggga actgataata tggatgatat tttaaaaatt
     5821 ttagataaac ataatgtaaa agcgaccttc tttttagttg gtagttgggt ggatgataat
     5881 gaagaaattg taaaagctat agatgaaaaa ggacatgaaa ttggaaatca ttcaaatact
     5941 catgccaatt taaaggaaat atcaaaagaa gacataacaa aagagataga aactacatca
     6001 gaaaaaatat ataatataac aggaaaaagg actaatttat ttagacctcc ttttggggac
     6061 gtaaacaata aagctatgga catttgtagt gatttaggtt ataaagtaat taagtgggat
     6121 gtagattcta tagactggaa ggagcttgga ccaaatcatg taattgaaaa agtaataaaa
     6181 gaatctcaac ctggttcaat agttttattc catgctaata taaatgatgt agataattat
     6241 ttggatacta taataagtcg actaaaaaaa gatggataca gtctagtcaa aatatctgat
     6301 ttgttgtata aagataatta tgtagttgat tccaatggag tgcaaaaaac taagaattaa
     6361 taagtaataa atgtatattt taagtaaagt tgtgataaat tatatagtat atgttagaaa
     6421 agtgtcgata aaaagacggt gtctattttt tgacactttt tatacatgta tgcaatatca
     6481 ataattatag attaaattga ttaaaaaagt gtctaataat tgactttttt ttgatgggtt
     6541 taaaaattta gaagaattaa tgaatgctgt aagacagtat ttccaaaggg tataagtttg
     6601 tttaaaagtt ggcatagaaa ttgctttgtt aataagcgat atgttctata gaaataaaca
     6661 atatgttaag accttataat agaaaaatag cattaacatt atttgaacag cttataaaaa
     6721 aattaatata cttaaggggg cgtactagtt gaacaaacta tattctattt tattaaaaca
     6781 acacgttggt ggtccagata agccagtagt aagtgttgga gatgtagtaa aaaaaggaac
     6841 tctaatcgca gaaccaactg gattaggggc aaatatttat gccagtgtta gtggaaaaat
     6901 aagtgaaata aatgaccaag cgatagtaat tgaagctgat gaagtacaag aagatacttt
     6961 tgaaccatta aaaggagaag gtatacttga cttaattaaa gaagctggag tagtaggaat
     7021 gggtggagca ggattcccta ctcatgtaaa attaaacata gatttaaatg gtggaacaat
     7081 attagcaaat gcagctgaat gtgaaccttt attagctcat aatataaaag aaatagaaga
     7141 aagaccagaa atagtttatc aaggtataaa atatgctatg gaagttacta atgctggaaa
     7201 aggtatgcta gcaataaaaa gtaagcatcc aaaagcaata gaagctttca aaaaagtaat
     7261 aaagccagga gacaacatag aagttgcaga actagttgat atgtacccaa tgggagaaga
     7321 gagagcaata gtaagagatg ttctaggaaa attacttgag ccaactcaat taccttcaga
     7381 ggctaatgca gtagtaataa acgtagaaac actaactaga atagtagagg ctgttgagca
     7441 aaaaagacca gttatatcta aaaatataac tgtagttggt caattaaaca gtggaaaaga
     7501 atctatagtg tttgaagatg tgccaattgg aacaactgtc ggagaattaa ttgaaagagc
     7561 tggtggaata aaaggtgaat atggtgaaat aatcctagga ggacctttca ctggtaaagc
     7621 tactacttta gatgctccaa taactaaaac tagtggtggt ataatagtta ctatgccatt
     7681 tgttaatgaa aagagaaaaa tgggtctttt agtttgtgca tgtggaccta atgaagaaag
     7741 aatgagagat atagcaacta aaatgggagt tactgatata gtatcagttc aaaaatgtaa
     7801 acaagctcaa gaaattaaag gtgcattaaa atgtgaaaat ccaggacact gtccaggaca
     7861 agctcaaaaa tgtattgaat ttaagaaagc tggagcagaa gttatattaa taggaaactg
     7921 tactgactgt agtaacacag taatgggttc tgcaccaaaa ttaaaattag gtgtatatca
     7981 tattactgac catgtaatga gaacagttaa tcacccatta ataagaagaa taaaaggtga
     8041 cgtaagatta aaaataaaat aatagataaa tagaagttag acatctttag aagtaaaact
     8101 taaaagttac ttctaaggat gttttttaat gtattttgag ggcaatatat atattgtaaa
     8161 ataaatggaa aaaatagaca aaatatgcta gatattgtat aatgataaca atagctatta
     8221 caattaatat tggacaaaaa taatgatact caagtatgtg gtttgtatta atatgcatat
     8281 tatattaagt caactatccg atagagtaaa aggtttttgt gatatttgaa gatactctat
     8341 agtatctttt ttattataaa aactaatttt tgtcttttat tagataagta tctaggatac
     8401 taatttatat tttattgagt tgttaaaaaa gtgcacttaa aaagttaaaa aagaaaaatt
     8461 aaaaacctat ttatagaaac tgtgaagtat atcttaaacc tgggcactta aaagatatat
     8521 ggagttagta gtgcaacctg ctataaatat aaataggagt aacttttaat tgtcaaaaat
     8581 atataaaata aatacataaa taagtaatta atagaagata gatataaata tatttttgaa
     8641 atgctccctg cattcataac gagtgtagag tggcagatat ctgcccaaaa ctcctttaat
     8701 tgctggaagt tcctaaagct aactaaacca caacataatc atgaaataag gataagtgtg
     8761 atagagtgtg catttaataa gcacttgagg ttacgaaagt agaaaaaatt agttagatag
     8821 cataaggtta aatcctaagt gccttgaaaa tggataatca gcaggtaagc ttcgaaaaga
     8881 agaaacttca acgactagag agaaatctcg tacctacaag tgtggggaaa tggggagtac
     8941 ctaatttatg gttaggtaat gatatagtct gtgcttaata gaaatattaa gaagttcata
     9001 ggagaactgg ttaggagtaa cgaccctaat tgaacacact aaagatgtat tattgaataa
     9061 gattcatata atatttaata attttaatta tattaagtga atacaatata tgtttttagt
     9121 atcaggagaa ttttgaaaac aaaaattaaa aaatcaagta taatcagttt agtaattgct
     9181 tttagtatgg tatttactgc atttacacca atagtatctt atgctgacga agtgactagc
     9241 aatgatacaa tacttaatgg agaagaacaa tcaaatggtc agacatcaga tgtagagaaa
     9301 ccatcagacg gtcaggtacc agatggggag aaaccatcag acagtcaagt accagatgta
     9361 gagaaaccat cagacggtca ggtaccagat ggggagaaac catcagacag tcaagtacca
     9421 gatggggaga aaccatcaga cggtcaggta ccagatgggg agaaaccatc agatggtcag
     9481 ataccagatg gggagaaacc atcagatggt cagataccag atggagagaa accatcagat
     9541 ggtcagatac cagatgggga gaaaccatca gatggtcaga tgccagatgg agagaagaca
     9601 tcagatagtc agacgccaga tgtagagaaa ccatcagatg gtcagatgcc agatggggag
     9661 aaaccattag atgagcaaac tccagaagaa gaaaaaccat tggaagaaga aatagttata
     9721 gaggaaatga gtttaaaaca agatattgat aaaattcttg atatgacttt atctcaaata
     9781 aataaaatag tatataactt ttgggaagat gaagaagatg taaaggcaga tgaacaatca
     9841 gaaattaacc aagtttttac ttcagaagac tcatttatta gcttatggta tgacaaaaaa
     9901 gcaaaagtaa aaaattcttg tttactaaaa gaaatagatg gttcagatag accttatcat
     9961 gatttaagat ttgatgatat tacaaagact ttaacttttg actatgtaat aaagggtctt
    10021 gtaggagcaa gctcaaaaga cgacaaatat gtaatagaag gtgaagatgg agagcaaact
    10081 gcattttgtt acaataatca tttaagacca ccaagtagta atggaaagag tccatattta
    10141 ccagcagaag attttaatgg tcaagaaaac caaaatgaag aagcagttaa atctatacta
    10201 tatgcaggtt ctgaatttga tggatttgga tataagcaac aatttaattt aggtggagaa
    10261 gaaaatgaat tgatgactta ctcagctact caaagtgcaa tttggattat tcttggacaa
    10321 atggatgaac aagaaagact taagcagtat caaggttcta taaacctttg tgataaactt
    10381 atagaaagag caaaaactga agaggaaaga gctgagtatc agaagaagaa agaagtagcg
    10441 ataaatataa aagcttattt agaagcattg ttaaaagcag gtcgtgaaga gttaaagcca
    10501 aataatacag gaaaacctag tttgagtaat ggtttaacta agattaactt tgaaaaaaat
    10561 gaagatggta cttatgaaac agaagctgta gccttagtag gttatagtgg tgtagtaaag
    10621 ttaaaactac cagatggagt aactgcttat gatggagatg gtaatataat aggtacaggc
    10681 gaagtagaaa tttcaacgca acagaaattt aaacttaaga gtgttggaaa accagatgcc
    10741 aaagctaata tttctgctgt atcttatgat tatatttttc caaaagctat tcaatattat
    10801 aaggcagtgc ttgatttagg acaaaaagac cattcatctt cattaccatc tagcaagcaa
    10861 aatttattaa gttatacaat agagaaaaag aatggtcaag aagttaactt taatataggt
    10921 ttaccaacag atgatgatga tgttgtaaat ccaccagtgc caccaataga tgatgatgtt
    10981 gtaaatccac cggtgccacc aacagatgat acaaatggac ataagccaaa accatcacca
    11041 ccaatagatg atacagtaat aaatccacca gtaccaccaa tggatgatac aataataaat
    11101 ccaccagtac caccaacgga ggatgcagtg ttaaatccac cagtgccacc aatggaggat
    11161 gcagtgctaa atccaccagt gccaccaaca gaggatacag tgttaaatcc accagtacca
    11221 tcagtaagtg atacagtgga aaaaactcca gaactatcaa gagatgatac aataataaaa
    11281 tcaccaaaaa caggtgatga aactcagcta atgtcatatg tatttattag tgtcattgca
    11341 atttgtggat tagcgtatca atgtaaaata aaaagaaatt aatctagatt attattaagt
    11401 ttaaaataaa aagtctcaaa ttgatttata agtcattttg gggctttttt atgtaaatat
    11461 aatatgcaaa tatagtttgt ttaaaaatat ctattttaat tatattctta aatttattat
    11521 tggatacagg cattcattaa ttgtttgatg aatagaaaaa tgttttgaag gtgaaaatag
    11581 tattgaaaaa ataatatact aagggtataa ttcatttgat ttcatttgta taaatttaat
    11641 tataaatttt aaagtgatta tataaattta aatgctaatt acttaataaa ttttaataat
    11701 aattatttaa taaataatta ttataatatg ctagaataaa tatatataaa taagttttga
    11761 tgaggtacta ctataaaggg gggactttat gttttcgata ccagaagtaa aaaaagtaga
    11821 agaggtaatg gatacaaaat ttacaactat agacgaagac acaagaattg aagatgcaat
    11881 aaaagaaatg ataaaaagca acacaaaaac tctaatggta atagattcat ctgaccaatt
    11941 aaaaggcatt atatccatga cggatataca taatctttat gagatgcata aaaagtatga
    12001 aggtcaacca gtaaaactta taatgaagaa ggatgtaata tatgtcaacg aaggtttaac
    12061 cttagatgaa tgtagagata taatgatatt aaaaaatata gggattcttc ctgttcttag
    12121 agataataaa attattggag ttctaaaaca agagcacatt agggactatc tatatatgca
    12181 tttagaagat tatggattaa ctttgaagta tattataggt caaattaaag aaggaatttg
    12241 tgctataaat aacgaaggtg ttgttatctt gtggaataag tttatggagg aaaggtatga
    12301 tataaagtct gaagatatag ttggtcgacc gatgaatgaa tttttggaaa atactatttc
    12361 agaaaaagta ctaaatagta aagttggaat gagtgacttg tattttacag ataaaaaaga
    12421 aaatatgtat gcattagttc atgcaaatcc tatattttat aaagaagaat ttatcggtgt
    12481 tgtatgtaca gaagttgatg ttacagaagc taagatatta gctcttgaac ttgaaaaagt
    12541 aaatgataca ttaaaatatc taaagaacga agtcaaaaat ttatctaaag gtagttttga
    12601 taagatttta ggtaaaagtt ataaattgga aaaatcgaaa gcgattgcaa aacaagtggc
    12661 tagaacaaat agtagtatat ttatttgggg cgaaagtggt acaggtaaag aagtatttgc
    12721 aagagctatt catgattata gtgagagaaa agggcaattt atacctgtta actgtagtgc
    12781 cataccaaat gagttatttg aaagtgagtt ttttgggtat gaatcaggtg catttacagg
    12841 agcaagtaaa aagggtcgaa taggtatatt tgaacttgca aaagatggaa ctgtattttt
    12901 agatgaaatt gccgatttac ctcttagtat gcaagcaaag ttgcttagag tattacagga
    12961 aaaagaaata agaagagttg gtggagatac cacaataaaa ataaatccta gaataatatc
    13021 agctaccaat aaagatttga gtaaaatggt aaaggcagaa aaatttagag aagatttata
    13081 ctatagatta aatgttgttg aaataaagat tcctccactt agagagagaa aagaagacat
    13141 aggattattg gttcatagct ttttagagga gatatgtaaa caaaataata agcctgttct
    13201 tacaattagc aaagatgtaa tagatatatt tcagaattat agatggaaag gtaatattag
    13261 agaattaaaa aatacgattg aaaatattgt tgtattgtca caaaattcta aaatagatgt
    13321 ggatgatgta ccaagttata tgatggattc tacaaataat agtactgaag aagaagagta
    13381 tcctttagat ttaacgaagg caactcaaaa aattgaaata aagaatataa ccaaggcact
    13441 taaaatgtct aatggaaata aagctaaagc agctaagata ttaaatatac ccagaacaac
    13501 tttgtattat aagatagacc aatacaaaat agatgtgtct aaaatatgac acatgactaa
    13561 attttgacaa aatgataaac tttaatttaa aacctagtaa agccaacact tgtataagtg
    13621 ttgtgtatat agatgtgtta aaaaaaatga aaaagtgttg attaattgac actttttttg
    13681 tttgagaaaa tgtttacaaa tacaaaaaat aacatatttt aaataaatat ttatataata
    13741 tttggctaaa aatagcgata gatgaagaat ctattataga aaaaatcttt ttgttaataa
    13801 gttggcatag gaattgctta taatataaag tgactttaag tgagaaaaaa ataatattaa
    13861 tcataacaaa ttatgtaggt tttaatttac ttaatttctc agttaagggg ttacctgttg
    13921 aggaattgag atttaaaata ataaataaga aaataatgta aggaggtatt ttatactatg
    13981 tcaataactt tagaaacagc tcaagcccat gcaaatgacc cagcagtttg ttgttgtaga
    14041 tttgaagcgg gaacaattat agcgccagaa aacttagaag atccagcaat atttgcagac
    14101 ttagaggatt ctggattatt aacaatacca gaaaatggat taactatagg tcaagtacta
    14161 ggagctaagt taaaagaaac tttagatgca ctttctccaa tgactacaga taacgtagaa
    14221 ggatacaaag caggagaggc taaagaagaa gtagtagaag aaacagtaga agaagcagct
    14281 ccagtatcag aagcagcagt agttccagta agcacaggag ttgcaggtga aacagttaaa
    14341 atacacatag gtgaaggtaa gaacataagc ttagagatac ctttatcagt agctggtcaa
    14401 gcaggagttg ctgctccagt agcaaacgtt gctgctccag tggcaagtgc agcagcagaa
    14461 gtagctccaa aagttgaaga aaagaaactt ttaagaagct taactaaaaa acactttaaa
    14521 atagataaag ttgaatttgc tgatgaaact aaaatagaag gaactacttt atacatcaga
    14581 aacgcagaag aaatatgtaa agaagctaat gaaactcaag agttagttgt agatatgaag
    14641 ttagaaataa taactcctga taaatatgaa acttacagtg aagctgtatt agatatacaa
    14701 ccaatcgcta ctaaagaaga aggcgaatta ggttcaggta taactagagt tatagatgga
    14761 gctgtaatgg tattaactgg tacagatgaa gatggagttc aaataggtga atttggttct
    14821 tcagaaggtg agttaaatac tactataatg tggggtagac caggtgctgc tgacaaaggt
    14881 gaaatattca tcaaaggtca agtaacaata aaagcaggaa ctaacatgga aagaccagga
    14941 cctttagctg ctcaccgtgc atttgactat gtaactcaag aaataagaga agcattaaag
    15001 aaagttgaca actctttagt agttgatgaa gaagtaatag agcaatacag aagagaaggt
    15061 aaaaagaaag ttgttgttat aaaagaaata atgggacaag gtgcaatgca tgataaccta
    15121 atattaccag ttgagccagt tggtacatta ggagctcaac caaacgttga cttaggaaac
    15181 atgccagttg tattatctcc acttgaagta ttagatggtg gtatccatgc attaacttgt
    15241 ataggacctg catcaaaaga aatgtcaaga cattactgga gagagccatt agtaataaga
    15301 gctatggaag acgaagaaat agatttagta ggtgttgtat ttgttggttc tccacaagta
    15361 aatgctgaga aattctatgt atctaagaga ttaggtatgt tagttgaagc tatggaagtt
    15421 gatggagctg tagtaactac tgaaggtttc ggaaacaacc atatagattt cgcatctcac
    15481 atagagcaaa taggtatgag aggtatacca gtagttggtg taagtttctc agctgttcaa
    15541 ggtgctctag ttgttggtaa taaatacatg actcacatgg tagacaacaa taagtctaag
    15601 caaggtatag agaatgaaat attatctaac aacactttag ctccagaaga tgctgttaga
    15661 ataatggcta tgcttaaaaa tgctatagaa ggtgtagaag ttaaagctcc tgaaagaaaa
    15721 tggaatccaa atgttaaatt aaataacata gaagctatag aaaaagttac aggagaaaaa
    15781 atagtattag aagagaatga gcaatctcta ccaatgagta agaagagaag agaaatatac
    15841 gaaaaagacg aaaactaaaa tttagatact aactaagatt tagacaataa cataaattta
    15901 gatccaagtt taaaacagta tagagaggta gaattataat ggataggtta aaatatatct
    15961 catcagagac tttttatgaa ggtattatag ttgacatcaa aggcggtgga gtgactatag
    16021 acctaaaagg tagacttgga caatttaaaa tacctaatag aatgcttata actgactacg
    16081 aacttaagat aggtcaagaa gttggattca tgctaagcta tcctgaggta ttaagtccag
    16141 agcctaatca agagtatgta gaaaatatta ggagagaaaa agaaaaacaa gcagaaatgc
    16201 aatcaaaaca agaagaaaaa taataaataa tcgtttgaaa atagaaggga gaggaaatat
    16261 atgagcctta caacagtaca aggacttcaa tctgaaatat tcgttccaat aacacctcct
    16321 ccagtttgga ctcctgtaac taaagaatta aaagatatga ctatagcttt agctacagca
    16381 gctggtgttc atttaaaatc tgacaagaga tttaatctag caggagatac tacatttaga
    16441 gctataccta atacagctac agttgatgaa atgatggtat cacatggtgg atatgacaat
    16501 ggagatgtaa acaaagatat aaactgtatg ttccctatag atagattaca tgaattagct
    16561 gcagaaggat ttatcaaagg agtagctcct atgcactatg cattcatggg tggtggagga
    16621 aaccaacatg tcttcactga agaaactggt cctgctatcg ctgctaaact taaggaagag
    16681 ggagtagacg gtgtagttat gacagctggc tgaggtactt gccatagaac tgccgtgatc
    16741 gtgcagagag caatagagga agctggaata cctacaataa taatagcagc tcttcctcca
    16801 gtagttagac aaaacggaac tcctagagca gttgctccat tagttccaat gggtgctaat
    16861 gctggtgaac cacataatat agaaatgcaa acacatatat taagagatac attagagcaa
    16921 ttagttgcaa taccatctgc tggtaagata gttccattac catacgaata taaagctcac
    16981 gtttaaaatg taatttatat ttgaaaatat agaacccttc tcctacacgg ttaggagagg
    17041 ggttttaaga acaaaggtgg atattatgga agaaaaaata atgagaagac ttgttataaa
    17101 accgtttcat atgaatgaag ttaatttcgg ttcaaaaact tctataaaga aagatgtact
    17161 tacaatagat ttatcaagta tagatgaaat aaaagaaaga gaagagttaa taacagacat
    17221 aaaagtagac attataaaac ctggagatta tgacagagaa attaatacta ttatggacat
    17281 tataccaata tcaacaaaag ttttaggcag actgggagag ggtattacac atactttaac
    17341 gggtgtatat gtaatgttaa ctggtgcaga tgaagatgga aatcaaatgc atgaatttgg
    17401 ttcttcagaa ggaattttga aagaccaaat ggtatttggg agatatggaa ctccatcagt
    17461 tgaagattat ataatccatg tagatgtaac tttaaaggga ggtttacctt ttgaaagaac
    17521 acttccactt gcagcattca gagcatgtga tgattttata caagaaataa gagcgtcttt
    17581 gaaaatggaa gacggaagaa atgctactca agttcgtgaa tattttgaca aaattcgacc
    17641 aaatgcaaaa aaggttgtca tagtaaaaca aatagcaggt caaggtgcta tgtatgacaa
    17701 tcagttattc tcaaaggaac ctagtggatt tgaaggagga acatcaatta tagatatggg
    17761 aaatgtacct atgattatat ctcctaatga atacagagat ggtgccctta gagctatgac
    17821 ttaagagagg tggatattat gggaatagga ccttcaacaa aagaaacatc tcttcatcac
    17881 tttagagacc cacttttaga catagttgaa agtgataagg atgtagattt acttggagta
    17941 atagtagtag gaactccaga tggaaatgaa aataaaacct ttgtgggaca aagaacggcc
    18001 gcttggctgg aagccatgag agtagatgga gctatagttt catcagatgg ttggggaaac
    18061 tcacatgttg actacgctaa cacatttgag gaaataggaa aaagagatat acctgtagtt
    18121 ggagttacat ttaatgggac tcaagctaag tttgttgtaa gcaatcaata tatggatacc
    18181 atagttgata tgaacaaatc aaaagaaggt attgagacag aagtagttgg agaaaacaac
    18241 acaaatgaaa tagatgctaa aaaagctttg gcatttttaa aacttaaaat gagaaaacat
    18301 ggataaaaaa tttaagtaag ggaatgagtt ttgtgggaat ttctacatca atgaaaaaca
    18361 catcacttca tatatttaga gacccgttac tagatttgat agataatgat tctgacatag
    18421 acttgttagg agtggttgta gttgggacat cagagcacaa tgaatggaaa acatttttag
    18481 caactagact tggaagatgg attgaatcat tgcgacctga tggagtgatt ataactttag
    18541 atatggcagg aaaccagcac attgatttta caaatgctat agcagagttt gtaaaatcag
    18601 atatacctac agtaggtctt actattatgg gagcagatgg attagtaatt accaatccat
    18661 agttggataa agctaccata attgattata aaaagacaac aggctacata gaaacagaag
    18721 tagttggaga caatcacatg gatgaagttg atgttaaaaa agcattggct ttcctaaaat
    18781 taaaaatgcg taaagacgca aaataaacct aagggggaat ttgatatgaa atttagcaga
    18841 agtatacaag ctatagactc tcatacagca ggtgaagcaa ctagaatagt tgtaggtgga
    18901 atacctaaca taaaaggaaa ttctatgcct gagaaaaaag aatatttaga ggaaaatcta
    18961 gattatttaa gaactgctat aatgttagag ccaagaggac ataatgatat gtttggttct
    19021 gtaatgactc aaccttgttg cccagatgct gactttggaa taatcttcat ggatggtggc
    19081 ggatacctta acatgtgtgg ccatggtaca ataggagcta tgacagcagc tatagaaaca
    19141 ggtgtagttc cagcagtaga gcctgtaact catgtagtta tggaagctcc agcaggaata
    19201 ataagaggag acgttacagt tgtagatgga aaagctaaag aagtatcatt cttaaacgta
    19261 ccagctttct tatataaaga aggtgttgaa gttgacttac caggtgttgg aactgttaaa
    19321 tttgacatat catttggtgg aagcttcttt gctataatac atgcaagtca attaggttta
    19381 aaaatcgaac ctcaaaatgc tggtaaatta actgaactag ctatgaaact tagagatata
    19441 ataaatgaga aaatagaaat acaacatcca actttagctc atataaaaac tgtagactta
    19501 gttgaaatat atgatgagcc aactcatcca gaagctactt acaaaaatgt agttatattt
    19561 ggtcaaggtc aagttgacag atctccatgt ggaactggaa caagtgctaa attagctact
    19621 ttacatgcta aaggtgaatt aaaagtagga gaaaaattcg tatatgaaag tatattagga
    19681 actttattca aaggtgaaat agttgaagaa actaaagttg cagatttcaa tgctgtagta
    19741 cctaaaataa ctggttctgc ttatataact ggatttaatc attttgtaat agatgaagaa
    19801 gacccactta aacatggatt tattcttaaa taagtaataa ataaattagg aaattaaaat
    19861 agaatagtag ccacttgtcg gttgctattc tatagctttt aggctataag attcagaata
    19921 tatgaaaaaa taaaaaaata ggaggatgga ttatggtagc aatcttaaag gcattatatg
    19981 tagtattcgc agcaatcttt gcagttgtgt ttggtaaaga cgtagctaaa gcaaacaaag
    20041 aggggaaact agaagaacaa agtactccaa aagttgctgt aacaggattt ataacagact
    20101 tctttgatac attaggaata gggtcatttg ctccaacaat agcaatgtca aaggctttaa
    20161 agttaaatat accagataaa aaaatgcctg gtacattaaa cgtagcacat actattccag
    20221 ttgttacaga agcattcata tttacatcaa tcataccagt tgatggtgtt actttagtat
    20281 ctatggttgt ggcagcagca gttggttcat atataggtgc aggaataata gctaagatgg
    20341 acgagagaaa aatacaactt gttatgggtg taactttagc attaacagca atattaatgt
    20401 tattaggtca tccttggatt aacgtattac caggtggagg aaatgaatta ggtcttactg
    20461 gtgttaaatt agtaataggt gtaataggta actttatact aggagcttta atgacagctg
    20521 gtataggttt atatgctcca ggtatggcaa tggtttattt cttaggaatg tctgcaaaag
    20581 ttgcattccc aatagttatg ggttcttgtg cattattaat gccagttgca agtatgaaat
    20641 tcataaaaga agatgcatat actaaaaaag catcagttat aatagctata tgtggtttaa
    20701 taggagtttt cgtagctgca tatatagtaa aaactctacc aatggatata ttaaaagcat
    20761 tagtaatagt agttatagct tatacttctg ctacaatgat aatggctgca aataaaaaca
    20821 aaaagactgc ttagttaaag actacttagt gtttattaaa tattacagct aataataaag
    20881 attataaact gctttaaaat attgaattaa tattttaggg cagtttttta tttactaaat
    20941 taatataaag caattaacat gccaattatg taatatatat agtgatttag tagatttaat
    21001 gtaaaaaacg ttgtattttt aaagaataaa gcataattta aagctaaata tttaaatttt
    21061 ataatcaata agttcaataa atgttcatat attaatacag attaataaaa ata
//
LOCUS       DAEMGG010000063        21078 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEMGG010000063 DAEMGG010000000
VERSION     DAEMGG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.063"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            223..1647
                     /locus_tag="KPK24_002197"
     CDS             223..1647
                     /locus_tag="KPK24_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG2299868.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANESEEDSTSLEEAKAKMSDMKSASKNSEKNIEKKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLDIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1682..3811
                     /locus_tag="KPK24_002198"
     CDS             1682..3811
                     /locus_tag="KPK24_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG2299869.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFSLASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIIK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKGKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNTKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIMSIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDE"
     gene            3841..4263
                     /locus_tag="KPK24_002199"
     CDS             3841..4263
                     /locus_tag="KPK24_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2299870.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAK"
     gene            4282..5433
                     /locus_tag="KPK24_002200"
     CDS             4282..5433
                     /locus_tag="KPK24_002200"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2299871.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEIYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEAHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSNGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5539..5640
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5823..6395
                     /locus_tag="KPK24_002201"
     CDS             5823..6395
                     /locus_tag="KPK24_002201"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG2299872.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKEEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6459..7109
                     /gene="fsa"
                     /locus_tag="KPK24_002202"
     CDS             6459..7109
                     /gene="fsa"
                     /locus_tag="KPK24_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG2299873.1"
                     /translation="MIFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHEGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7261..10185
                     /locus_tag="KPK24_002203"
     CDS             7261..10185
                     /locus_tag="KPK24_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2299874.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKEPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPVLNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            10762..11220
                     /locus_tag="KPK24_002204"
     CDS             10762..11220
                     /locus_tag="KPK24_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2299875.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            11281..11556
                     /locus_tag="KPK24_002205"
     CDS             11281..11556
                     /locus_tag="KPK24_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2299876.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            11615..12889
                     /locus_tag="KPK24_002206"
     CDS             11615..12889
                     /locus_tag="KPK24_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG2299877.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            13019..14071
                     /locus_tag="KPK24_002207"
     CDS             13019..14071
                     /locus_tag="KPK24_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2299878.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            14071..15102
                     /locus_tag="KPK24_002208"
     CDS             14071..15102
                     /locus_tag="KPK24_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG2299879.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            15194..16006
                     /locus_tag="KPK24_002209"
     CDS             15194..16006
                     /locus_tag="KPK24_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG2299880.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            16009..16929
                     /locus_tag="KPK24_002210"
     CDS             16009..16929
                     /locus_tag="KPK24_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG2299881.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            17104..17550
                     /gene="rpiB"
                     /locus_tag="KPK24_002211"
     CDS             17104..17550
                     /gene="rpiB"
                     /locus_tag="KPK24_002211"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG2299882.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            17580..18245
                     /locus_tag="KPK24_002212"
     CDS             17580..18245
                     /locus_tag="KPK24_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG2299883.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            18355..19857
                     /locus_tag="KPK24_002213"
     CDS             18355..19857
                     /locus_tag="KPK24_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG2299884.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRTYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(20169..>21078)
                     /locus_tag="KPK24_002214"
     CDS             complement(20169..>21078)
                     /locus_tag="KPK24_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2299885.1"
                     /translation="IVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKY
                     KLEVIGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILS
                     YVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRE
                     LLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTR
                     VYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNII
                     ELENAHMEIIIDADLFKVMLTFRKVN"
BASE COUNT         8537 a         2213 c         3912 g         6416 t
ORIGIN      
        1 aaatttaaat attttataaa aatagtatta tttacaaaat catatactcc aaaatactac
       61 ataaataaaa ttaaacaaaa actaaaatga caaatgtcat tatctaaagt gacatagaaa
      121 cggtttctta aagttgttac tataaaatat aaactctata ataaaagtat aacaaaaggg
      181 aatcatatcg atatagaata taattcaaga ggaggtaaaa ttatgagtag ttctaatcaa
      241 tcgacaataa agacatctat acagcgagta gggaaatttt taagtggtat ggtaatgcca
      301 aatataggag cttttatagc atggggtctt ataacagcat tatttatacc aacaggatgg
      361 atgcctaatg aaaaattgag tactataggc gacccaatga taaaatacct tttaccacta
      421 ttgatagctt atactggtgg taaagcaata gcaggacaaa gaggtggcgt aataggtgca
      481 gcagcagcta tgggagtaat tgttggggca gatataccaa tgtttatcgg agcaatgata
      541 atgggaccat ttgcaggatg ggtaattaag aagttcgata aatttgtaga tggaaagata
      601 ccaacaggat ttgaaatgtt agtaaataac ttttcaatag gtataatagg tatgcttctt
      661 gcaatactag gattttatgc aattggacca gcaatagtag cagggacagc tcttatagaa
      721 tctggagttc aatttatagt tagcaaaagt ttattgcctt tagtttcagt atttatagaa
      781 cctggaaaag tattattctt aaacaatgca ataaatcatg gtatattagg gcctataggt
      841 atagctgagg caaaagaagc aggtaaatct ataatgttct tactagaaag taaccctgga
      901 ccaggtttag gagttctatt agcatattgg atgtttagta gaggaagtgt aaaacaatca
      961 gcaccaggag ctgtaataat acatttcttt ggtggtatcc atgaaattta cttcccatat
     1021 attttaatga atccagtttt aatattggct acaatggcag gtggagcagc aggaatattg
     1081 actttttcaa tattaggagc tgggcttgta gcagcaccat caccaggaag tatatttgca
     1141 cttatggcac tagctccaaa gggaggatta cttccagtat tagcaggggt tgcagtggca
     1201 acagttgtat cattcttagt ggcagcacca tttgttaaga gagcttcagc aaatgaaagt
     1261 gaagaagaca gtacttcttt agaagaagct aaagcaaaaa tgagtgatat gaaatctgca
     1321 agtaagaact ctgagaaaaa tattgagaaa aaacagcttg aagtaaatga gattaagaag
     1381 attgtatttg catgtgatgc aggaatgggt tcgagtgcaa tgggagcttc tagatttaaa
     1441 aatagaataa aaaacttaga tttagatata gaaattacaa attcatctgt tgataatctt
     1501 ccagatgata cacaaatagt agtaactcac aatacattag ttgaaagagt tgctaagaac
     1561 aattcaagtg ttgagatagt ttcaataaac aattttttaa atgacccaaa tttagataca
     1621 ttatttaaaa gattagaatc taaataaaaa taaaaagtac cactaattag gagtggataa
     1681 tttgaaaaaa aagaagatta catctaggca aaaaaagata atactaatga tagtagaaaa
     1741 ttcaaagaaa aacattccaa taactatttc tgagattgca gggacgttag agttaagttc
     1801 aagaactgta cttagagata tgtctggaat tgaaaaatgg tttgatgaaa atgattttaa
     1861 ctttgtaaaa aaaccaggag taggattgat tttagaggaa aatatagaaa atcaaaactt
     1921 tataattgag ttgttagaag aagaaaaaat agaaaaagaa tacagcaagg aagaaagaaa
     1981 tttaattata ttatcaaagc tactagtttc taacgagcca gtaaaatctt attattttgc
     2041 aaaaatatta aaggtatctg aaggggtact gaataatgat ttttcattag caagtaaatg
     2101 gttggaaaga tttgatattg agcttgttag aaaaccaggg cttggtgtct acctaaaagg
     2161 tcaagaaaaa agctttagag aggcatatgt aaatcttata tacgattcat tcaatgaaaa
     2221 agaaatacta gatatggtca gaaatataag tgaaaatata caaacagata aagcaataga
     2281 aattttaagt gaaaatagac ttttaaattt aatggatagg tgcataataa taaaagttga
     2341 agctacatta acaaagaagc tttcagattt agatgtaaat ttagctgata gtgcatatat
     2401 agggcttgta gttcatattt cattagcctt acagaggata aaaaatggtg aaaatataac
     2461 tatggataaa gagtttctaa aagaattatc tataacagaa gagtttaaat tagctaaaga
     2521 aatcgtaaaa ggtatggaaa cagattttaa catggatata ccagtagatg aggttggata
     2581 tataactatg catataagag gtgcaaagca aaggtcatct tcaaatcata aggctttaaa
     2641 cttggatgat atagaaataa tggaaattac aaataagatg atagatttgg ctgaagatga
     2701 atttaaaata agtttaaaaa atgacgagag gttatttaaa gacctagcaa atcacttagg
     2761 accttctata aatagattga atatgggatt agaaataaga aatcctcttt tagatgaaat
     2821 aaaaagcaag tattcatatg cctatgatgg agttgaaaaa atttctagaa ttataaaaga
     2881 taaactaaat ataaattcaa tacctgaatc agaaatagga tatattgcaa tgcactttgc
     2941 atcagccata gaaaaaaatc ttatgatgaa tactaatata aatattgttg tggcatgtcc
     3001 cactggaata ggtacttcaa gattcttatc aactaagatt gaaaataaat ttcctaattt
     3061 aaatatactt gaaaccatat cagctataaa catagatgaa gagtatttaa agggaaaaga
     3121 tgttgattta atagtgtcta cagttgagtt aaatacaagt ttaaattata tatgtgtagg
     3181 tccatttatg agtttagatg atgaacagat aatcaaggaa aaaataaaat ctatagctca
     3241 aaataaatta attaatttaa atacaaaaaa tgatactaaa agtaagaata aagtatatga
     3301 acagataaca gaaagtatga atataggaaa agatattctt caatttttag aggaaattag
     3361 atttgaaaaa tttgaaagta aagatttaag tgagcttatt gaagactcat caaaaatatt
     3421 tgcaaaatct agtgaagata taatgtctat aaaagaatcg ttaaaagaaa gattaaaaat
     3481 ctcaattcca tatatagaag aatcaaaaat tctattatta cattgtatga gtgaaagaat
     3541 agatattatg aagttatcaa taataaaact ggagaatcga atcgtgctag attcaaatga
     3601 agaaatcgac aatgtagttt ttatgttact tcctaaaaat tcaccaagtt atcaaagaca
     3661 aataatgagt gaaataagtg gttcattaat agacaatata atttttacta ataagataaa
     3721 taaattctct atagaagaga tgacattaga ggtaaaagat atagttttta atttttatac
     3781 aaacagatta aaagcattta tagatgaata aaattaatat taaaaaggaa ggttgatact
     3841 atgagtaaag tattgaatga gaataatata tttttagggt tagatagtgt ttcaaaggaa
     3901 gaagctataa ctttagcagg aagaaaatta gttgaaaatg gatatgtaaa agaagaatat
     3961 ataccagcaa tgctagaaag agaaaaagta atgactacat atatgggaat gggagttgct
     4021 attccacatg gtgtaaatga agcaaaaaaa gaaatattat cttctggaat agttatacta
     4081 caattcccta atggtataga ttttgatgga gaaaaagcat acttattaat aggaatagct
     4141 ggtgttggag atgaacattt agaaatacta tctaatatag cagtagtttt agatgatgac
     4201 ttaacagaaa gactaaaaaa ttcaaatgac aaacaagctt ttatggaagc ttttgccaaa
     4261 taatcttagg agggatttaa aatgaaaaag gcaatccagt ttggagcagg aaatattgga
     4321 agaggtttta ttggaggctt gttagttaag tcgggttatc atgtagtatt tgctgatgta
     4381 aatgaggaga tattaaactc aataaataaa gataaaaagt atactataca cataagagat
     4441 gtagaatgta tagatgaagt aattgataat ataagtgcgg tttcatctat aaaagaagaa
     4501 ataatagatg agattgtaca ggctgaaata ataactacag cagttggacc tttagttttg
     4561 actaaaatag cctctacaat tgcaaaaggt ataaaggcta gaaaagaaaa gggtttaaca
     4621 agtaatctaa atataatagc ctgtgaaaat gcaatatacg caagttcttc tttaaaagaa
     4681 gaggtactta agtatttaaa taaggaagaa gtagaatatt tagagatata tgttggtttt
     4741 ccaaactgtt cagttgatag aatagttcct cctggtaaaa atgaaaatcc tcttgatgta
     4801 acagttgaaa atttttatga gtggaatgtt gaaaaacaag gatttaaagg ggaaatacca
     4861 actatagttg gaatgaattt agctgacaac cttatggctt atatagagcg taaattgttt
     4921 acactgaata caggtcatgc tataacagct tatataggat atttaaaagg atataagact
     4981 atagaagaga gtataaaaga taaatttatc tgtgatatag tcaaaagtgc aatggtagaa
     5041 agtggagaag gattgattaa aaaatacaat tttgactcag aagcgcacta taaatatatt
     5101 gataaaattt taaatagatt taaaaatcca tatcttaatg atgatgtatt aagagttgga
     5161 agggaaccat taagaaaact aagtgataaa gatagattga ttaaaccttt aatgacagca
     5221 aaatcatatg gattatcagt tgacaatttg attcttggaa taggtgcagc gcttcattat
     5281 aataatagtg aagatactca aagtgtagaa ttacaagagt tgataaagtc taatggtgta
     5341 aaaaaagctg ttgctaaaat agctaatata agcaatgacg aagaactttt aaataatatc
     5401 gaaaaaagtt atatatttat gaaaaattta taaatttact tgaaaaaaag tagttctgat
     5461 aaagtaacta taatatatta ttctacacag tttgaattat ttgttgaata atagagaatt
     5521 ataatatata attaaaagga ataaattaat atatttacct gtatatgttt gataatatgg
     5581 ttcaaatgtt tctactaagc taccattaat agcttgacta caggagtgaa gaagatatag
     5641 tttatactta ttttaaatta tatattaagt taattacttt gaattttaag ctcctgtcta
     5701 tgatatagac aggagctttt ttagtatttt aatataaaat acttacaaga atagatagca
     5761 aaattagaac aaatataatt tttttattaa tataacttta gaaagggcta ggtggaagtt
     5821 caatgaaggc tttacaagaa aagatattga gagaaggcag tgtttcagga aatgatatac
     5881 taaaagttga tagtttttta aaccatcaaa ttgatgtagc ttttttaaat gaaataggaa
     5941 gagaatttaa agagagattt aaagaagaaa aggtggataa gatatttaca atagaggctt
     6001 caggaatagc tatagcatca attgtttcac agtattttga taatgcacct gtagtatttg
     6061 caaaaaaatc agaatctaaa aatttggata aagatgtata tgagactaat gtatattcat
     6121 ttactaaggc aagagaatat tcagttaaag tttctaaaaa gtacataaac aaaggtgaaa
     6181 atatattgat agtggatgat tttttggcaa atggaagagc tgctcttgga cttaaagatt
     6241 taattgagca agcagaagct aatttagttg gagtaggaat tgtcattgaa aaaggatttc
     6301 aagctggagg agcactttta aaggctaatg atgtaagatt agagtctttg gcggtagttg
     6361 aatctataga taatggaact gtaaaattta ggtaatttat gatattatat acttgtgttt
     6421 agcacatatt ttaactatgt aattatggag gattttttat gatatttttt atagacacag
     6481 ccaatattga agaaataaaa gaagcaaatg atttaggtgt aatatgtggt gtaactacta
     6541 atccatctct aatagctaaa gaaggtagag attttataga ggtagttaaa gaaataagtg
     6601 aaatagtaga tggaccaata agtgcagaag taataagttt agagcatgaa ggtatgatag
     6661 aggaagcaga aaaattatct aagatacata aaaatatagt tataaagata cctatgacag
     6721 cagaaggtct aaaagcggtg aaagtactat caagtaaagg tataaaaact aatgtaacac
     6781 ttatattctc tgctggacaa gctcttttag ctgctagagc tggagctaca tatgtaagtc
     6841 cttttgttgg tagattggat gatatatcac aaaatggtct tgatttaatt gaagaaatcg
     6901 ttgatatatt tagtgtaaat agtattgagg ctcaaataat agttgctagt gttagaaatc
     6961 caatacatgt tttacaagct gctagaatgg gagcagatat agcaacagtt cctcttaaag
     7021 ttataaacca aatgattaag catcctttga cagacaaagg aatagatagc tttatgaagg
     7081 attgggaagg tgcttcttta aaattataat tataaactac gctttataag tatattaaag
     7141 aggttattat ggaaattcta agtaataagg ctaggctatt ttgcctagtc ttattttggt
     7201 gtataatata ttgaaacata ttattgaaat agattaattt tttaagtgag gaggcgaaaa
     7261 atggataaaa tattaagaga gatacaaaga gaagataaaa aaaatccata tactgaccaa
     7321 gaacttgctg aaatgttaaa tgtagctagg tctgaagtga tatcagtgag gaaaaaaaat
     7381 aatattttag attcgagaga aagaagaaaa aaaatattaa taaaagatat atcaaaaatt
     7441 gtaagtgaaa atcctcaaat gtctgaaaga aaaataactg agatattaat agagaatgga
     7501 tataatatat cgagaagtgc agtttcaaaa ttattaaaag aagaaaattt aaatcagtca
     7561 aagattggga ttaaagataa agagccagca tcatcaggaa agtatgtgaa ggtgagtaaa
     7621 gttgtagaag aagatggatt tgaagaattg ataggtataa agggaagttt gaaagaaaag
     7681 gtaaatctag ctaagtcagc aataatgtat cctcctaatg gccttcatac tataatatat
     7741 ggagaaacag gggtaggtaa aagtgaatta gctacatgta tgtataaata tgctataaaa
     7801 aacaacatca aagaagagaa cagcccattt atagtgttta actgtgcaga ctatgcagaa
     7861 aatcctaatc tattgatagc tcaactcttt ggggtagtaa aaggtgcata tacaggagct
     7921 gatgcaaata gagaaggtct tgtagaacaa gctaataatg gaatattgtt tttagatgaa
     7981 attcatagat tgcctgctgc tgggcaagaa atcttatttt cattaataga tagaggagaa
     8041 tttagaaggt taggagaaag tagttctaat agaaaggcaa atgttctcat aataagtgct
     8101 acaacagaaa acccagattc taatttactt caaacattta gaagacgtat accaatggta
     8161 ataaatttac ctaatatgag tgaaagacca aaatctgaaa gatatgaaat aataataaag
     8221 ttttttgaaa gagaagcaaa gcgagtaaac aagaatttta taataactaa agaagttatg
     8281 tgtgcactta tgaattataa gtgtactgga aatataggtc agttaaagag tgatatacaa
     8341 gtaacttgtg ctagagcttt tagtaaatca atattttcta gcgataaggt aattatagat
     8401 ttagatagtt taagggatta tataaaaagt ggatattatg attgtaattg taaaaatgaa
     8461 aagtattctg agttttcagt tgaggatata tatatagata tcagtgaatt ggtgagtgga
     8521 aaaaatagaa ttttatctga taatgagata agcgagatat ataattatgc agaaaaagag
     8581 cttaaggttt tggaaagtaa gtgtttttca gaagaagagt taaaaaatgc atttatagaa
     8641 aagttagata agaagtttaa tgctattaaa aacaataaaa atttatctga tagaagaagt
     8701 cgaatagact ggggaggaca attaaaagag tctacattgg agataatgga taaggtaatt
     8761 gtatttatta aggagcagtt taagcatgta aatcaaggat tgtacttggc tctagcaatt
     8821 cacattgagc atgctatagg taggataaaa gatggaaaaa ctataataaa tccgagttta
     8881 gataaaataa aagcatcaat gccaatggaa tatgaattgt caagatatat aatgggtata
     8941 gtggaagatt tgacacaagt aacatttcca gaagatgaac ttggatattt ggcttattat
     9001 ataaataaat tttgttataa tgaggaaagt ataaaagata aagttaaagt agtaatagtt
     9061 acgcatggta aagttggaat tgaaatgtct aaagttgtaa atcatatttt aggtatagaa
     9121 tgtacactag gtatagaaat agcacttaca gattcaccat cagaggggat agagcatgta
     9181 ttagaagagc ttcaaaagat agaagctaga aaaggtattc tagttctaat tgatatgggt
     9241 tctttggtaa ttcttggtga tgaggttgaa aaaagacttg gaattagatg taaaactgta
     9301 aacagagtag atacgttatt ggcaatggaa gcaggaaaac ttgcaaccat tgaaggaaaa
     9361 agtttagatg gtataatagc agatttaaag aaaaataaga attatgccat gataaataca
     9421 aataaattta gttacagaaa aaacgagtat ggaaagaaaa atgtaatcat tactttatgt
     9481 ttgagtggtg taggtacagc tttaaattta aaagagcata tagaaaaaca aattaaggaa
     9541 tatgacactt taattgaggt aaagccagtt gcttttttaa ataataattt agaagaagag
     9601 ttaaatgata ttgaagctga atataatatt attgctatct gtggaactat cgatatagat
     9661 tataaaaacg taccttttat ttcttataat gaggtacttg gtaaaaatgg tattaataaa
     9721 gtattagagc atttaaacag taaacaacca gttttaaata ttgaaaacca gttgaataat
     9781 ttaatacatg aagatttgat tttatacaat tttgaaggaa tttcaaagga gtatataatt
     9841 gatactcttg tttcaaagct tgaagaaggt ggatatgttg actcaaagta tatattaagt
     9901 gtatataaaa gagaagcaat gggaagtgtt gtaatggcct ctaaggtagc cgtgccacat
     9961 ggacttccag aaaacgttat aaagcctgca atagcaatcg caaggttgaa taagcctata
    10021 gtgtgggaca ataaatttat ggtggatttg gtagtactac tggcacttaa agaaaataat
    10081 aagaaagaaa tacgaagctt attttcaaag attaatgacc aacatacttt agaagtttta
    10141 ttaaatactg aagataaaaa tgaaataaaa gaacttttaa gctaattatt agtacagaga
    10201 agaaagacaa aaaatttgct ttagtataaa atttaaaact acttaattgc taagtagtta
    10261 attactcgat ataaggctag ttctatgaaa tttatttata tgattttata gaattagctt
    10321 ttttcttggt aaataaaata tatgaattta tttatgcaaa aagtaaattt tattggacaa
    10381 ttctatgaga atttttccta tatttcttat aaaatataga atatataaaa ataaattaaa
    10441 ttcataataa ataattttat aaaatagttg ttaaacataa aaaaccattt gtaaaaaaca
    10501 aaaaaatcat ttctctaaca aaaaaaacca caaaaaatat aaaaaagtat ggctagttaa
    10561 gtgattaaca agtaaaaatt aaaataaaaa tcaaaaaaaa tttatcaatg aaagtcagct
    10621 aaaatcctgg aagagagcat tatttttggg aaaacatttt cttttgtttc acaaaaaaag
    10681 ccactttttg gcacacaaca tgctttatat aaagtcaaga aaaactaagg tataagacaa
    10741 aggcaattag gaggtgtaaa tatggagttt ataaattttg taagtgataa agttatagac
    10801 cttaaaatgg attgtaaaac gaaagaagat ttctttaaag aaatacataa taaagttttt
    10861 gatttaggct ttgtaaaaga agaatttgga gaaaaaatat tagcaagaga aaatgtattt
    10921 ccaacaggtt taaatttagg agattatgga gtagcaatac cacatacaga tgcagaatat
    10981 attaaagagc aatttatatc agtctgtact tttaatgaac cagtagtgtt tagttctatg
    11041 gaagaccaag atgaaaaagt acctgtaaat ttggctttcg tattaggact taatcaacca
    11101 cacagtcaat tatctgtatt aacagaatta atgggaatta tgcaaaataa agagttagtt
    11161 gataagttga tgagttctac tgataaagaa gaagttttaa agacactaaa gtctttataa
    11221 aaataaaaaa gtactaaaaa taaaaaatat aaaaataaaa attattagga ggaaataaaa
    11281 atgaaaaaaa tattagtagc atgtggagca ggaatagcaa catcaacagt agtttgtgac
    11341 aaggtagaga gattagttaa ggaaaataat atacaagcag aagttataca atgcaaaata
    11401 gctgaatgtg caacaaagca agaaggagca gatttaatag tatcaacaac tatactacca
    11461 acaacttata atataccagc aataaaagca acagcatata taactggagt aaatacagca
    11521 gcattagata aaaaaatatt agatgcttta aaataatcct atcatagata cattgaaata
    11581 atattttata aaacagaaaa aggaggattt ataaatggat aaattattag cagtagtaca
    11641 atacatttta aatatgggac cgacagtaat actaccaata acaattgtag tactaggaat
    11701 gatatttaga attaagttta caaaagcttt aaagtcaggt cttacaattg gtataggatt
    11761 tgtgggtata aacttagttg taaacctatt aacatcaaca ctagggccag cggctcaagg
    11821 aatggttgaa agattcggac ttaatttaac agtaatagat gttggttggc caacaacagc
    11881 atcagcaaca tgggcttcac cagttgcacc aatattaata ccagtttgtt tattagtaaa
    11941 cttactatta ttatacttca ataaaacaaa gatattaaac gttgatatat ggaattattg
    12001 gcactttata gtagcaggtg cagttggata tatagtaaca ggaagttatg tttgggctat
    12061 attctgtgca atactaatgg agttattagt actgtttatt gcagaaaaga cagcaaaaaa
    12121 tgttcaggag ttttatgatt tagaaggtat agcattacca actggttcaa cagcttcatt
    12181 tgctccactt ggatatttag taggtaaatt tgttgaaaaa ataccaggaa taaataaaat
    12241 acaagcagac ccagactcta tacaaaaaag atttggtata tttggagaac caatgatgat
    12301 ggggattata ctaggtataa tacttggagc acttgcggga tatgatgtag caggaatatt
    12361 caatgtaggt atatcaatgg gtgcagtaat gttacttatg cctagaatgg ttagaatatt
    12421 aatggaaggt ttaattccaa tttcagaatc aataagagat ttcttacaag caaaatatgg
    12481 agacagagaa ctttatatag gtctggatgc agcagtagca actggacatc cagcagttat
    12541 atcaacagct cttatacttg ttcctataac tttgttctta gcagttatac taccaggaaa
    12601 caaagtttta ccatttggag acttagcgac aataccattt tatatggcat ttatagtgtg
    12661 ttttagaaaa ggtaatatag ttcaatcagt tataacagga acaatagtaa tagctatatc
    12721 actttatatg gctacaaact ttgcagatgt tcatacacaa ttattacaac aagcaaatat
    12781 gatgccagaa ggaacaacaa tggttacaag tatagataca ggtggaaact tgctaaaatg
    12841 gggaatattg aaattatctc aattagtttc ttcatttgga atattctagt atgtagttaa
    12901 tagagattac tcttttttag taaagtatgc gaaaatttaa catttagcag ctactaaaaa
    12961 agagtaatat tttttataaa tttttaaaaa tacaaaatac aagaggagga gatttatcgt
    13021 gaaagcagca gtattacatg gcacaaatga tatgagattt gaagatatag aaataaaacc
    13081 atgtgaaagt gatgaagtaa aaataaaagt tatggcagca ggaatatgtg ggtcagatcc
    13141 tccaagagta ttaaaacatt ggaaatatcc agttccagct ataccaggac atgagttttc
    13201 tggagtaatt gctgaagttg gtaaggatgt aaaaaatgta aaagttggag atagagtagt
    13261 tgcaattcct tttatacctt gtaatgaatg tgaatattgt aaaagaggat tattttcatt
    13321 gtgtgatgac catggaatgt taggagctaa aagttttgga gcttttgcag agtatgtaaa
    13381 tataaaagct acaaatgtac ttccaatagg agatatgaac tttgaagatg ctgcaatgat
    13441 agagccacta gcagttgcta tgcatggagt acttaatata ggtgttcaag ttggagacac
    13501 tgtagctgta atgggtagtg gaacaatggg gcagttggta atacaaggac ttaaaatagc
    13561 aggggcagga acaattatag cagttgatat atctgataat aaattaagag aatcaaagga
    13621 attaggggct gatataataa ttaatgctaa agatattaac ccagtagaaa aaataaaaga
    13681 acttactggt ggaaaaggtg ttgacatagc tcttgaatgt gcaggttcaa aaataactca
    13741 agaacaatgt ttacttataa ctaaaaagaa aagtaagata ggatttttag ggatagcata
    13801 ttcagatata actttatcag aagaagcatt tgaaaatata tttagaaaag aactagaatt
    13861 aaaaggtttt tggaactctt attctgcacc atttccagga caagaatgga ctaagggaat
    13921 caatctagtt aatgaaggaa agataaaatt aaaagaaatg gtatctcata gattctcact
    13981 tgaagatact tacaaagcat ttgaaatgat aagagataga aaagaagaat ttaacaaaat
    14041 attaatttta ccacaagggg ttgaaaaata atgaaagcag tagttaaaac taaaccaggc
    14101 tatgataatg tagaagtttt agaagtagaa gaaccaaaag ctacaggaga caaggttaaa
    14161 ataaaagtag aatatagtgg tatatgtggt tcagatatac attcatttaa aggtgaatat
    14221 gcaaatataa aagccccagt aacattaggt catgaatttt ctggaattgt tgtagaagta
    14281 ggagaagatg ttaaaaatat aaaagtaggg gatagagtta catcagagac tacttttgca
    14341 acttgtgaga aatgtatata ttgtgaaact aaagattata acttatgccc aactagaaaa
    14401 ggtattggta ctcaagtaaa tggaagtttt gctgagtatg tattatcaag agaagaaagc
    14461 atacatgtac ttcctgaaaa tgtatcttta ttatcagcag cccttacaga gccactagct
    14521 tgttgtgttc atgcagctct tgaaaagact actatagaaa gttctgatac agttttaata
    14581 ataggaccag gaccaattgg tttattatta gctcaagttg ttaaatctca aggtgctaca
    14641 gttataatgt ctggtgttac aaaagataag gaaagattag aaatagctaa aaatcttgga
    14701 gtagatagaa ctgtaaatgt aatggaagaa agtttggcag atgtagtgaa tgaaatgact
    14761 gatggaaatg gagtaaacaa aggatttgat tgttctgggt ttatgccagc agttaacgaa
    14821 gccttaagac tgcttagaaa aaaaggtact ttcgtacaag ttggaatctt tgctaaaaaa
    14881 ttaaatgaaa tggaccaaga agcaataatc caaagagagt tacaatatat aggaagcagg
    14941 tctcaaaagc caagttcttg gattacagca ttagatttaa tggcaaatgg gaaagtaaat
    15001 acagaagctt tagttactag aactgttggt ttagacgaat ttaaagaagg tattacagct
    15061 ttaatgaacg gggaagaaat aaaagtagca gtaaaatctt aaaaaaacat ttgtattatt
    15121 ttaaaaaaaa ttgtaaaatc tactgtagga taaaaaaatg taaggtctta aaaaataaca
    15181 ggaggaagaa agtatgagca ctcttagaga tcattctaat aatattagaa aaaacataat
    15241 aaaaatggtt gcagaagcta aaagcggaca tccaggtggt tcattatcaa tagctgatat
    15301 gttgacagta ttatattttg ataagatgaa cgtatgtgca gaaaatccaa aaatggctga
    15361 tagagataga tttgttctat caaaaggtca tgcagcacca gctttatatg ctacattagc
    15421 agaaaaagga tttttcccag aagaagaatt aataacacta agaaaatttg gttctaagtt
    15481 acaaggtcac ccagacatga aaaaagtagc aggaatcgat atgtctactg gttctcttgg
    15541 tcaagggcta tctgcagcaa atggtatggc acttgcagga aaattagata acaaagacta
    15601 tacagtatat actatattag gtgatggaga aatccaagaa ggtcaaatat gggaagctgc
    15661 aatgacagca tcacattaca aattagataa tttaattgca tttgttgact taaatggatt
    15721 acaaatagat ggttctaatg aagaagtaat gaatgtaagt ccagtagatg acaaattcaa
    15781 atcttttgga tggaatgtaa tagtaataaa tggacattct tttgaagaaa taggaaatgc
    15841 agttgatgaa gctaaaaaag ttacaggaaa accaactgta ataatagcta aaacagtaaa
    15901 aggtaaaggt gtttcattta tggaaaataa tgctgcatgg catggaacag ctccaaatgc
    15961 agaacaagca gagcaagcat tgaaagattt agaaggaggt ctataggaat gggaatagca
    16021 actagagaag cttacggaca agtgttaaaa gaacttgcag aaaataaaga tatagtagta
    16081 ttagatgcag atttaggaaa agctacaaaa agtatatcat ttaaagaagt tgccccagat
    16141 agattttttg acatggggat agcagaaggt gacatgattg gtacagcagc aggtcttgct
    16201 acttgtggaa aaataccttt tgcgagtact tttgcaatat ttgcagcagg aagaggctat
    16261 gagcaaataa gaaactctgt agcatatcca aatttaaatg taaaaatagc agctactcat
    16321 gcaggtgtaa cagtaggtga agatggtggt agccatcaag ctatagagga tatatctcta
    16381 atgagaggta taccaaatat ggttgtatta aatccagcag atgctttaga agcaagacaa
    16441 gctattctta catctattga ttataatgga cctgtttata taagattagg tagagctgca
    16501 actcctgatg taaacagtga aaattataaa tttgaaatag gaaaaggaac tgttttaaga
    16561 gaaggttctg acataacagt aatagctaca ggaataatgg tagcaaaagc cttagaagca
    16621 gcagaagaac ttgctaaaga aggtataaat gtagaagttg ttaatatatc tacaataaaa
    16681 ccattagatg aagcattaat aaaagaaact gctaagaaaa ctggaaaagt agttacagca
    16741 gaggagcaca gtataatagg tggtttaggt tcagctgtat gtgaagcttt agcagaaact
    16801 aaagatgtag tagttagaag aattggtgtt aaagatgtat ttggtcaatc aggtacacca
    16861 gctgatttat taaaacacta tggtttaact acagaagata tagtaaaaaa tataaaagag
    16921 ttgttataga tataaatata tctaaataac aaattgattt gacataagtc aattaaagct
    16981 gtcttaaaaa tagaaagaaa tttctacaat gggacagctt tatttttaca atactttatt
    17041 tttatgataa ttataaaata gtctgaaagt aatcaaaaaa attataaaaa gaggtgctat
    17101 ttaatgaaaa tatctattgg atgtgaccat ggtggttatg aattaaagga atatataaag
    17161 gaaaatttaa taaataaagg tatagaagtt gaagatgtgg gtacaaattc aaaagaaagt
    17221 gtagattttc caatatatgc aaaaaaggta agtcaaaatg ttcaaagtaa aaaatctgat
    17281 ttaggcatac tttgttgtgg aacaggaata ggtatgtcta ttgcagcaaa taagtttaaa
    17341 ggaatcagag cagctgttgt ttctgactgt ttttcagcta aggcaactag agaacacaat
    17401 aatacaaatg tactttgttt aggagaaaga gttattggga aaggattagc aatgagaatt
    17461 gttgaagaat ggttaaattc aagttataat ggagaaagac atgcaagaag attacaaatg
    17521 attgatgata tagaaaaaga aaatttataa atatataatt tacaacaagg gggagcgtta
    17581 tgtctattat ttgtgcttca ataatgtgtg cagaccaaat gaagttaaaa gaggaattag
    17641 aagctcttga agaagcaggt gtaaaattac ttcattgtga tgttatggat ggaatatttg
    17701 ttaaaaactt ggctatggga ccagagttat taaaatcaat aagtgaaaat acaacaatac
    17761 ctttagacat acacttagcc acagaaactc cagataaata tattgatatg atgtcttata
    17821 taaagccaaa atatatttct tttcatgtag aatcaagtac aaatgtaaaa gctgatatac
    17881 aaaagttaag aaattatggt atagggccag tgttagcaat aagccctcaa acaagtgtgg
    17941 ataaaataga agaatatata agtttagttg agggtatctt agtaatgaca gtaaatccag
    18001 ggtttgcagg tcaaaagttt aatctttctg tattggataa gttggataaa cttacagaga
    18061 tactaaaaga ttatgataat cctccattta tagaggttga tggaaatata aataaggata
    18121 ctattaaact tatgaatggt aaaaaagtag atatatatgt tgttggtact tcagctttat
    18181 ttaatgataa gcctccaatt tcatataaag ataaaataga agaactaaaa gaaagtataa
    18241 agtaaccatg taataaaatt ataatatgtt tctgtttaat agtaaaaatt taggtggaaa
    18301 caagaaattt tataatattt aataaaaatt ttggttgagt aaggagaatt ttttatggga
    18361 gacctatata aactacaaaa tggtactgat attagaggaa tagcgtatga aaatgattct
    18421 aaagaagtta acctaactgt agaagaagta aaaaaaatag ctaaagcgtt tcatatttgg
    18481 ttaaaagaaa agactaagaa agataaagta actgttgcca taggaactga ttctaggata
    18541 acaggaagcc aatttagaag tactgttatt gaaactttaa ctaatgatga ttgtaatgta
    18601 attgattgtg agattgccac aactccagct atgtttatga caactattat ggatggatac
    18661 aattctgatg gggcgattat gattacagca agtcacttac cttattacta taatggttta
    18721 aagtttttca cagaaagtgg aggtttagaa aaaactgata taaaagagat gttggatata
    18781 gctgttaaaa atgatagtgt agactatgag gaagtaaata aaaaaggtga agttatagta
    18841 aaaaatttaa tagaagatta ttctaattta ttgatagata aaattagaaa aggtgtaaat
    18901 tcaagtaaaa attatgaaaa gcctttttct gggcttaaga tattggttga tgcaggaaat
    18961 ggggctggtg gattctttgc tgaaaaagtt ttacacatac tgggagcaga tacgacaggt
    19021 agtcagtttt tgaacccaga tggtatgttc ccaaatcata tacctaatcc cgaaaacaaa
    19081 gaagcaatgg aaagcatatg taaagctgtg ttggataata agagcgacct aggaataatc
    19141 tttgatacag atgtagacag agctgctatt gtaggtaaaa atggcaaacc aataaataaa
    19201 aatgctctta tagctgttat atcatcaata gtattagaag aacatccaaa aacagctata
    19261 gtaacagatt cgattacttc tgaaggactt gctaaattta taaatgaatt acaaggaaga
    19321 catcatagat ttaaaagagg atataaaaat gtaataaatg aagctataag attaaatagt
    19381 gaaggtgaag agtgccattt agctatagaa acgtctggac atgctgcttt aaaagaaaat
    19441 tatttcttag atgatggggc atatttaata gctaaaattt taataaaagt agcaaagcta
    19501 tccttggaag gaaagactat agaagaattg atagaaaacc ttgaagaagc tcaagaagag
    19561 aaagaagtta gaataggcat taataaagaa gattttagaa catatgcaga ggatatatta
    19621 aaagatttag attctcatgt taagagtatt gatggatggt ctgtagcacc aaataatttt
    19681 gaaggcataa gagttaattg tgatgaatca aatggagatg gatggttttt acttagaata
    19741 tcactacatg aaccattatt agctttaaat attgaatcaa ataaaattgg cggagcagaa
    19801 caaatttatt ctaagttaaa acttttctta gaaaaatatg atttaaaagg tctttagggt
    19861 aaatagaaag aatatattta tattgaagaa taaatgtaaa tttgtaccta agattacaaa
    19921 tattacaaaa ttgtaatctt aggtaacaga gatgtaactg attatggaaa cactatgtga
    19981 tattatagat acagagagat gaaatatgtg atgaattata gagaaatata atttaaaatg
    20041 tattaatatc acaccaaaaa ggtataatca aaaaatatgt aaatataaac cccaacattt
    20101 agaaaaagac ctctaaggag gtttttttct tgtttaaata acttaaaata tatttttata
    20161 ttaatatatc aattaacttt tctaaaagta agcattacct taaataaatc agcatctata
    20221 attatttcca tgtgtgcatt ttctaattca attatatttt gagcaattga taaaccaaga
    20281 ccagagccat ctgtttttct agatatatca ccccttctaa atctttctat taactcttct
    20341 ggttttagat ttagtttatc atttgaaata tttttaaacg ttaatattat attttcttcc
    20401 tttataaaca tatctatata gaccctagtg ttaggcatag agtattttag tatattgcaa
    20461 attagattct caaaaactct ccaagttttt ttaccatcta tcatggtaaa aacagcatat
    20521 tcaggtgttt ctattataaa atctaaactg ctattttgaa ttttgtcttc aaattctaca
    20581 atactttgaa ttaaaagttc tcttatatcg gtttttgtta tattaaattc tacatctcca
    20641 ctacttagct tagatgcttc aaatatgtca tctattaata attttaatct ttgtgatttt
    20701 ttatctaaaa tatttatata gtcatttata gtctctctgt caaagtcatt attttttaac
    20761 atatcaacat agcttaatat agatgtaagt ggagttttta agtcatgaga tacgtttgtg
    20821 ataagctctg ccttcatcct ttcatttttt atattagcat ctacagcagt ttcaagaccg
    20881 cctgttatat tatttatact tttggctaaa gtagatattt catcatctcc tataacttct
    20941 aatttatact ttaaattgcc attttctata ttttttatat tttttgatat gttatttatg
    21001 taattaaact taacagataa aaagttgtat atataaaaat gaaaaactgt gtttacaagt
    21061 gtaaataaaa ttactata
//
LOCUS       DAEMGG010000064        20450 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEMGG010000064 DAEMGG010000000
VERSION     DAEMGG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.064"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            445..1515
                     /locus_tag="KPK24_002215"
     CDS             445..1515
                     /locus_tag="KPK24_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299886.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1499..2368
                     /locus_tag="KPK24_002216"
     CDS             1499..2368
                     /locus_tag="KPK24_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG2299887.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2412..3272
                     /locus_tag="KPK24_002217"
     CDS             2412..3272
                     /locus_tag="KPK24_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG2299888.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3303..4607
                     /locus_tag="KPK24_002218"
     CDS             3303..4607
                     /locus_tag="KPK24_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2299889.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4670..5932
                     /locus_tag="KPK24_002219"
     CDS             4670..5932
                     /locus_tag="KPK24_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG2299890.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6119..8089
                     /gene="uvrB"
                     /locus_tag="KPK24_002220"
     CDS             6119..8089
                     /gene="uvrB"
                     /locus_tag="KPK24_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG2299891.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8108..10933
                     /gene="uvrA"
                     /locus_tag="KPK24_002221"
     CDS             8108..10933
                     /gene="uvrA"
                     /locus_tag="KPK24_002221"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2299892.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11192..13009
                     /gene="uvrC"
                     /locus_tag="KPK24_002222"
     CDS             11192..13009
                     /gene="uvrC"
                     /locus_tag="KPK24_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG2299893.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13009..13938
                     /locus_tag="KPK24_002223"
     CDS             13009..13938
                     /locus_tag="KPK24_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG2299894.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14152..15948
                     /locus_tag="KPK24_002224"
     CDS             14152..15948
                     /locus_tag="KPK24_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG2299895.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16088..17869)
                     /locus_tag="KPK24_002225"
     CDS             complement(16088..17869)
                     /locus_tag="KPK24_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG2299896.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17892..19778)
                     /locus_tag="KPK24_002226"
     CDS             complement(17892..19778)
                     /locus_tag="KPK24_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG2299897.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19795..20292)
                     /locus_tag="KPK24_002227"
     CDS             complement(19795..20292)
                     /locus_tag="KPK24_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG2299898.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
BASE COUNT         7896 a         2716 c         3504 g         6334 t
ORIGIN      
        1 gggtaaattt ataaaaatta aaaaattttt attttttatg tttaaacctt gtaaatttgg
       61 gacataatat aattgtaaaa cttaatatag caaaaataaa ttactcaatc tcccccactt
      121 aaagacctct atcccccata gaggtctttt ttactttcaa atcaaattat ataaaaaata
      181 aatagtttta tatgactctt cataaattaa aattacattg tgatagcaaa caatgaatat
      241 gaaaaatata gaaaattata gctaacataa gctgtatatc atcttaacct cagtatattt
      301 aacaaaaaat gtttataatt taacctttat tttaataagt ttaccatata ttcactttgt
      361 cttaatatat cctttacaaa ttaagagtat tattaatcta taagtaaatc gtagaattaa
      421 attaaatatt ttgggaggat aaaaatggca gatataagac tagaaaatgt aacaaaatct
      481 tataataaaa aaaatacagt tatagaaaat ttaaatctta ctatagaaga tggttctttt
      541 acagtattgg taggcccaag tggatgtggt aaatctacta cgttaagaat gattgcaggt
      601 cttgaagata taacttcagg taggttgaag attgaagaaa atgatgttac aaaaacggat
      661 gctagtaaaa gggatatagc tatggtattt caaaattatg cattatatcc acatatgact
      721 gttagagaaa acatagaatt tggtcttaaa aacaaaaaag tagaaaaaga aaaaagagaa
      781 aaacttataa cagaagttat tgatgtagta ggattgagag aatatttaaa tgtgaaacca
      841 ggcaacttat ctggaggtca aagacaaaga gtggcacttg ctagagctat ggttaaaaat
      901 ccaaaggtat ttttaatgga tgaaccattg tcaaatttag atgctaaatt aagaaatcaa
      961 atgagaacag aattaataca attacatcaa aaactaaagt ctacatttgt ttatgtaact
     1021 catgaccagg ttgaggcaat gtcaatggca gataagatag ttataatgaa caaaggaaag
     1081 ataatgcaaa taggttctcc aaagaagata tatcaaaatc ctaaaaattt atttgtagct
     1141 caatttatag ggagtccttg tatgaacata ctagatataa aaaatgatat taaatttgga
     1201 ttcagaccag aaaaagcaac actagagagt ataaacatag atggaaaaaa tgatgatgta
     1261 tttagtctat ttggaaaagt tgtaactaaa gaagtgttgg gaaatgaaac tatatattgt
     1321 ttatctgttc ttaatagaga gataagagtc aaaacagaaa atgatatatt tgatattgga
     1381 agaactttaa gaataagtgt attagaaaaa gatttatatt attttgataa aaatggagaa
     1441 agaatagaag atgtaaaagt atgtaaaagt atgtattcta aaattaaagg agatatttat
     1501 gaaaaagcaa tttaataaag catatttata tataacacct gctattatag gaactttatt
     1561 gtttatagtc tatccaataa ttaagacgat atctctaagc ttcaaatctg gtagtcttct
     1621 tagttcaacc tttgaaaatg taggcttaaa caattataca gaacttttaa agaatccaga
     1681 gtttatacaa actataacaa acactgcaat ttatactttt tttatggtga ctatatctat
     1741 atcactagcc atagtacttg ctgtttggct aaataaaaat tctataatac acaatttaac
     1801 gcaaagtata atctttacac cacatgtaat ttcactagta tctgtagctg tattgtggct
     1861 ttggattatg gagccacaat atggcttttt aaactggata ctatcgacat tacatttacc
     1921 aacatcaaag tggctttcaa gtgaaagttc agctttaatg tcactaattc taataggaat
     1981 atggaagact cttggatata atatcttaat agtgttatca ggtcttaaat ccataccaaa
     2041 ctatatatat gaagcatcta aattagataa tgcaaacaaa ataacgaatt tctttaaagt
     2101 gacattgcca ctattatcac caacaatatt ttttcttatg ataacaacaa caactgcatc
     2161 ttttcaagta tttgatgaga taagtataat gacacaaggt gggccagtgg gttcaactaa
     2221 catgttatct tattatatat atgagtctgg atttatatac tatgacatag ggcaagcgag
     2281 tgcagcgagt atgtttttgt tagctatagt tatgattgct acatatgcta actttagact
     2341 cttatcaaag aaagttcatt atcaataaag aaaaagtaga tattataagg tttatccaaa
     2401 aggaggagac aatggatatt atacaaccta tcaaaaaaga caaagttgat ataaaaaaaa
     2461 tagcatcaaa atcaatagaa atattaattc tattactttt atctgcaata tttatattcc
     2521 cttttatatg gatgctatct acatccttta aaaatccaca agaaatgatg caattaccgc
     2581 ccactattat accaaatgat atagtgttag aaaattatag tacagcatgg cattcaggtc
     2641 cattttttag atatttgcta aacagtgtat ttgtaacagg aagtgtttta attgtacaat
     2701 ttttagttat ggttccagca gcatatgcat tttctaagat taaatttaaa ggtgaaaaaa
     2761 tgctatttgg gcttttgctt gtaggactta tgattccacc acaagtaact tttctaccag
     2821 tctatatgat gatgagtaag tttggattta taaatactta tgttccactt ataattccat
     2881 ttatgacatc ttcatttggg atatttctgc ttagacagaa ttttatgcag atttcagatg
     2941 aaattattga agcagctaaa ctagataatg cttctgactt aaaaataatg tttagaataa
     3001 tggttcctat ggcaaaacca gcagtaataa catttatatt atttaatttt atataccatt
     3061 ggaataatta tttctggccc ttagtaatga caaatacaga tgcaataaga actttaccaa
     3121 taggagttgc gctccttaaa tcttcagaag gaattactgc atggaatgta ataatggcag
     3181 gaaatataat tctaatttta ccaataattc ttgtttatat atttgcgaat aaaaaagtaa
     3241 aagaagcttt tatgtattca ggaataaaat aaaaaattaa agactaaatt tggaggatga
     3301 aaatgaagct taaaagaaaa ttagcaacag gattagcaat aacattatta ttaggttctt
     3361 tagttgggtg ttcaaattct aatgatgcaa atgcatctaa aggagaaaag actgatagca
     3421 atattacgaa gatagatttc tgggctcctt taggtggaac taatggagaa actgctcaaa
     3481 aaatggttga ccaatttaat agtgaacata aggatgtaca agtaaatatg ttaaaacaga
     3541 aagattacta tgaaaatgca acaaaactac aggcagcctt aacatctaaa gaccaaccag
     3601 atgtaacatt attagaaata actcaaacag gtacattcgc atcagcaggt gctttagtag
     3661 atatgagtaa atattttgat aaaacatatc aagaaagatt cttttctggt cttttaacaa
     3721 attcatatta tgaggataag tttgttggta tgccatttaa tagaagtaca ccaatattat
     3781 acataaataa agatatggca actaaagcag gtcttgaccc ttcaggtcca aaatcatggg
     3841 aagagcttaa ggattatgct agtaaaatga caaataaatc agaagatact tatggttttg
     3901 aaacaccaat agatatttgg ttctatgaag ctatggtaat gcaaagtgga ggagaaataa
     3961 ctaatggtaa aaaagtagca tttaataatg aggcaggaca agcaccagtt aagttctggc
     4021 aagatatgat gaaagctgga ataatgaaaa tgcctcctgg agaagattat aatgcctggg
     4081 acgtagcaaa acaagatttt gtaaatggta aagtaggtat gatatttaca tcaacagcag
     4141 atttagcagg acttatgcaa caaacagaag gtaagtttga aatatcaaca gcattcttac
     4201 caaaaaatca aaaatacgca actcctacag gtggagctaa tctagttatg cttgaaggtg
     4261 gaacagataa agaaaaagaa gcaagtgcag aatttataga atggatgaca caaacagata
     4321 aaatcgttca gtttagttca tcaacaggat acttaccaac aactgaagat gcaacaaact
     4381 cagaaaagtt acaaacatta tataaagaaa aacctcaata taaggttgca actgaccaat
     4441 tacaatatgc tgttgcactt ccaatgttaa atggatacaa ggaagcaact gataagctaa
     4501 tggatgagat taaaaagggg cttactgatt taaatgcaaa tccaaaagat gtagtaagca
     4561 aaggaacttc tgcaatgcaa gcagtaatag ataaaacaaa taaataatta tatagacttg
     4621 taagttgatt aaaagtattc aataatatat aaatgaagag gtaatgtaaa tgacaaaggt
     4681 tatagtatta tcagtagatt ctttatttga aaaagacttg gaatttgtaa aaaatctacc
     4741 aaactttaaa tctatactag aaaactgctc catagtaaaa aatataaatt gtgtttatcc
     4801 cactctgaca tatccgtgtc atactagtat ggttacagga gtatatccta aaaaacatgg
     4861 tatatatcat aatgaaaaat ttgacccaaa taaagaaaat aaagactggt actggtactc
     4921 aaaggacata aaagcaaaaa ctattataga tgtagcaaaa gaaaataatc taactacctc
     4981 tagtgtatta tggcctgtaa tgggagcaaa tccaaatata gattataata ttgcagaaat
     5041 atgggctcct agtagagaag atgacccaag agaaactttt gaacgaagtt ccagtaaggt
     5101 tataatggac ggtatttaca atagacattg tcattatatc gattggaaat tagaacccaa
     5161 catggattta tttggagtaa actgttcagt agatataata aaggaatata aaccagattt
     5221 aatgctaata catttagcta cattggacca tacacgacac aattatggct tatttaatga
     5281 cgaagtagat aaagcattaa aaatgaatga taaatggtta ggagatatta ttcaggctac
     5341 aaaggatgca ggtacataca aagatacaaa ttttataata cttggggacc atggtcatct
     5401 tagagttgat aaagttataa atccaaatgt cttgcttaaa tcaaatggat ttataaaagt
     5461 tgaaaatgga gaagttaagg attttgatgc ctatgtaaat tcttctggaa tatcagctca
     5521 aataatagta aataacttag ataggttaac agaattaaga gaactgcttg aagaatttaa
     5581 agaagaatta ttcatagaga atatttttac caaagaggaa gcatctaacc ttggtcttga
     5641 aggtgatttt gaaatggtaa ttgaaggtct tgaaggaatt tcatttggaa atgatttcga
     5701 aggaagtatt ataaaagatt cagatataaa agattacaag tttgctgttg cgacacatgg
     5761 tcatttacct actaaaggta atagacctcc atttatagct tttggaccaa atattaaagg
     5821 tggtgtggtt atagaagaag gagacctgag agcacatgca gcaactatat taaaaatgtt
     5881 taatttaaaa ttagatggag ttgaaaaaga agcatttgat tttattatgt aagttgaata
     5941 aagtatttat tttccatgaa ttatagactt ctattgcaaa gatagaagtc tttttttatg
     6001 ttttagattt aagtaaaatt taaatgggta aaatatttaa aataacttaa acaattttat
     6061 aaaggtatga tataataata catgtcagga actaatgttc gaaaaaagga gtgatactat
     6121 ggattttaaa ataaaatcag acttcaaacc tacaggtgac caaccagaag ctattaaatc
     6181 aatagtggat tctataaata gaaatgagaa attttcgaca ttattaggtg taacgggttc
     6241 aggaaaaaca ttcacaatgg caaatatcat tcaacaagtt aagaaaccta ccctaataat
     6301 ggctcataat aaaactctag cagcacaact ttacagtgag tttaaagaat ttttcccaga
     6361 caatgcagtt gaatactttg taagttacta tgattattat caaccagaag catatgtagc
     6421 tcacagtgac acttatatag aaaaagatgc aagcataaat gatgaaatag ataaacttcg
     6481 acattctgct acagcatcta ttttagagag aagagatact ataataatat catctgtatc
     6541 ttgtatatat ggacttggtg acccaaaaga ctataaagaa cttatgttat ctataagacc
     6601 aggtatgcaa agagacagag atgatgtaat aaaaaggtta atagagatac aatacgaaag
     6661 aaatgacata aactttacac gaggtacctt tagagttaga ggagatatac tagaaatatt
     6721 cccagctagt aatgatgaaa aagcaataag aatagaattt tttggtgatg aggtagatag
     6781 aataacagaa attgattatg taacagggaa gattgttggt actagaaatc atgttgttat
     6841 attcccagca tctcactatg taacaacgcc agaaagaatt gaaaaggcaa ttgtagaaat
     6901 agaaaacgag cttcaagaac aaattaaatt tttcaaggaa aatgataggc tattagaagc
     6961 acaaagaatt gaacaaagaa ctaagtatga tatagaaatg ttaaaggaaa taggattttg
     7021 tcaggggata gagaactatt ctagacatat tacaggaagg tcagaaggag aaagaccata
     7081 tactttaatg gatttcttcc cagatgatta tttgataatt gtagatgagg cacatgtcac
     7141 tataccacaa gtaagaggta tgtatgcagg agacaggtct agaaaaacat cacttataga
     7201 gaatggtttt agattacctt ctgcacttga caatagacca ttaaattttc aagaatttga
     7261 aggaaatata aatcaaatgt tgtttgtaag tgctacacca gggccttatg aaattcaaca
     7321 ctcagagaca atagcagaac agataataag accaactggc cttttagacc caattgtaga
     7381 agtaagacct ataaataatc aaatagatga tttggtagga gaaataacta aaactataga
     7441 aaaaaatgaa agagtgctta taaccacttt aactaaaaaa atgagtgagg acttgactaa
     7501 ctatcttaaa gaaataggga taaaagttaa atatttacac tcagatatag ttacattaga
     7561 aagaacagaa ataataagag atttaagatt agggaagttt gatgtccttg taggaataaa
     7621 cttacttaga gaaggattgg atataccaga agtttcactt atagcaattt tagatgcaga
     7681 caaagaaggt ttcttacgtt ccgaaacagc tttaatacag actattggac gtgcagctag
     7741 aaatgaaaat ggaagagtaa ttatgtatgc agacaggata actgactcaa tgcaaaatgc
     7801 tattgatgaa acaaagagaa gaagagatat acaaaattta tataatgaag agcacaatat
     7861 cattcctaaa actatacaga aaaatataag agatagtata gaagctacta aggttgcaga
     7921 ggaagaagtt gtatatggaa tatctgatac agatgataaa gacgaaataa gagctaatat
     7981 agataaatta aaatcagaga tgatggaagc tgctcaaaac ttacaatttg aaagagcagc
     8041 agaacttaga gataaagtga aacaattaga agaaaaattg gaaaaataga aaggtaaaaa
     8101 caacattatg gaagataaaa ttataataag aggagcaaaa gaacataatt taaaaaatat
     8161 agatttagaa ttaccaagag ataaatttat tgtttttaca ggtttgtcag ggtctgggaa
     8221 gtcttctttg gcatttgata caatatatgc agaaggacag agaagatatg tagaaagtct
     8281 atctgcttat gcaagacaat ttttaggtca gatggaaaag ccaaatgtag aatatataga
     8341 gggtttatca ccagctatct caattgacca gaagactacc tcaaaaaacc cacgttctac
     8401 ggttggaaca gtaacagaaa tatatgatta tttgagacta ttatttgcaa gagtaggaga
     8461 tgtgtattgc cctacatgtg gtgaaaaaat aagtcagatg actattcaag aaatagttga
     8521 taaaatgcta gattttccag atagaacgaa attacaaatt ctttcaccaa ttgttagagg
     8581 acaaaaaggt acacataaaa aagtctttga taatatcaaa aaagaaggat ttgtaagagt
     8641 tagagttaat ggtgaaaact atgaagttag tgatgatata atcttaaata aaaatcaaaa
     8701 acataatata gaagttgtag tggacagaat agtaattaaa gatggaatag aatcaagact
     8761 agcagattca attgaaactg cagttaagct atctgatggt cttgtaatag cccaaataat
     8821 agatggagaa gaaattcttt tttcaactaa atttgcatgc ccagaacatg gaattggcat
     8881 agaagaatta tctccaagaa tgttttcatt taatgctccc tttggtgctt gtgatgtttg
     8941 taatggtctt ggggaaagta aagaagttga cccagaatta gtagtaccaa acaaagattt
     9001 atcaataaaa caaggtgcag tggcagcatg gggttcaaca ggtgttaatg atgacactta
     9061 ttacagtaaa atgataaaaa gtttagcaga gcattttaat gtatctttga caacaccgtt
     9121 taaagattta ccagatgatt ttgtggagga attactttat ggtaaagata atataattgt
     9181 agagtttact tatgagtcaa aatttggagg aactagaaat tataaatcat actttgaagg
     9241 tgtaatagta aacttagaga gaagatatag agaaactaat tcagattaca tgagagataa
     9301 gattgaagaa tacatggcag agagaccatg tccaaagtgt aaagggatga gacttaaaaa
     9361 ggaagtttta tctgttttag ttgatggaaa aaatataatg gaagttacaa acttatctgt
     9421 aaatgaattg attgacttta tggaaaatat aaatcttaca gaaaaacaga gatttatagc
     9481 tcatgagatt ataaaagaga taaaaggaag agctatgttc ttaagagatg taggtcttga
     9541 ttatttaaat ttatcaagaa aagctggaac tttatcagga ggagaagcgc aacgtataag
     9601 attagctact caaataggtt ctgcattggt tggagtttta tatgtacttg atgagccaag
     9661 tatagggctt caccaaagag ataatgatag gcttataact actcttagac atctaacaga
     9721 tttaggaaat accttaatcg ttgtagagca tgatgaagac acaatgagag aagctgatta
     9781 tgttatagac attggtccag gtgcaggtat acatggtggt gaaatagttg cacagggtac
     9841 tttagatgaa ataattgaaa atgagaactc aataacgggt caatatttaa gtggtaaaaa
     9901 acaaatattg cttcctgaga ctactagaga aggaaatgga agttttatag aaatagttaa
     9961 agcatcagaa aataatctga aaaatattga tgtaaagttc ccattaggta agtttacatg
    10021 cataacaggt gtttcaggtt ctggaaaaag tacattaata aacgatatac tttacaaagg
    10081 tgttgcaagt aaagtgaata gacttaagca aagagctggt aagcataagg agataaaagg
    10141 aattgaaagt atagataaag ttataaatat agaccaaagt cctattggaa gaacacctcg
    10201 ttcaaatcct gctacttata caggtgtatt tgattttata agagatattt ttgcatctac
    10261 taatgaagct aaagcaagag gttataaaaa aggtagattt agttttaatg taaaaggtgg
    10321 tagatgtgaa gcttgtaagg gtgatggaat aataaaaata gaaatgcatt tcttaccaga
    10381 tgtatatgtt ccttgtgaaa tttgtaaagg tgaaagatac aatagagaaa cattgcaagt
    10441 aaaatacaaa gataaaacta tatctgatat attagatatg aatgtggaag aagctgttga
    10501 gtttttcgaa aatataccaa atataaaaag aaaattagaa actttaatgg atgtaggact
    10561 ttcatatatt aaattaggac agccttcaac tcaattatca ggaggagaag cacaacgtat
    10621 aaaattagct gctgaactta gtaaaagacc aacaggaaag actttgtata tattagatga
    10681 acctactact ggacttcata tggcagatgt agacaagcta atccaagtat tacaaagact
    10741 agcagataca ggaaatacaa ttatagtcat agaacataat ttagatgtta taaagacttg
    10801 tgattatgta atagacttag gaccagaagg cggagataag ggtggaacta tagttgcaac
    10861 tggaacacca aaagaagtat ctaaagttga tggttcttat acaggtcaat tcttaaagaa
    10921 atatttttct taagttatat aaattgtgaa aaattttaga gcagatatac gataaactgt
    10981 tttgttataa gtaaattagt caaatataag ttgtaaatag taatacaaag taaagtccat
    11041 attactcttc atggttaaaa ttgttttcaa atcaattata gaagggaata tgggctttaa
    11101 cttgtttaaa gcactggaaa ttgtataact ttttaaaatt catattttag ggtataattt
    11161 tagtataaaa tttaatgttt tggagggcga gatgtttgat atacaagagc atttaaaaaa
    11221 attaccttct gagcccggag tatatttaat gaaagacaaa tacgaccata taatatatgt
    11281 gggaaaagcc atatctctaa aaaatagggt aaggcagtat tttcaatctt caaaaaacca
    11341 tacatctaaa gtaaaatcta tggttaagaa tatatataag tttgagtata ttataacgga
    11401 ttcagagtta gaggcattaa tacttgaatg taatttaata aaaagataca gaccaaagta
    11461 taatgtagtg cttagagatg ataaaactta tccatatata aaagtaacta cgaatgaaga
    11521 ttatccaaga atcttaaaag ttagaagagt tcttaaagat aaggcaaagt attttgggcc
    11581 ctataccaat ataactgctg tgaatgatac cttggagctt ataagtagta cgtatccaat
    11641 aagaagctgt aaaatagata tagataaagc aataaagaat aagacaagac cttgtttaaa
    11701 tctacacata aataaatgtt taggaccttg tacaggaaat gtttctaaag aagagtatgg
    11761 aaaaatgata gaagagatta taatgtgttt atctggtaaa gaagaaaaat tgatggaact
    11821 tttaaaggaa aaaatgaatg agagttctat gaactttaga tttgaagagg ctgctgtata
    11881 tagggataag ataaagagcc ttgaagagat gatacagaaa caaaaaatag atgctactgt
    11941 aagtgactta aaccaagatg tggttgcaat ggcaagagct cataatgaag cttgtgtcca
    12001 ggtatttttt ataagaaatg gtaagattgt aggtagggaa cattttatat tagaaggggt
    12061 aatggatagt ccaagagctt ctatattaag ttcatttgta aaacaatttt ataatgaaca
    12121 agagtatata cctaaagaat taattataga agatgagata gaagatagtt ctatattaga
    12181 agaatggctg tcatctaaaa aggggcaaaa agtaacaata agagttcctc aaaagggaga
    12241 gaagaaaagc ttagttgaaa tggttaggaa aaatgctgta gaatatcttg aaaaattttc
    12301 tgatatgaat aaaagaaaat atgaaaaaag tattggagca ttggaagaat taaagcaaat
    12361 attaaatcta gaaaagttgc caataagaat agaggcttat gatatatcaa atatacaagg
    12421 tgtagattcg attggttcta tggtagtgta tacgaatgct aaaaaagata agaaggaata
    12481 taggagatat aaaataaaga cagttattgg acctaatgat tatgattcta tggctgaaat
    12541 tgtagataga agattaaaac atggaaattt acctgacttg atattgttgg atggtggaaa
    12601 aggacaagtt agtgcagtaa aaaaagtact agaactcaat gatgtagata tacctctttg
    12661 gggaatgtac aaagatgata agcatagaac taaaggattg atatgcaaag aaaaagaaat
    12721 agaactagat aaaacaacta atttatatag atttattgct agtatacaag aagaagttca
    12781 taattatgca ataacatatc atagaagtct aagaaataaa gctttgacaa aatctatctt
    12841 agatgatata caaggaatag gtgaaaaaag aaagaaatca ttattaaatc actttaaaga
    12901 tgtagatgct ataaaaaaag ctactatgga agaactttta gaagtagatg gaatgaataa
    12961 aagcattgca gataatgttt ataatttttt tagaaaggaa gaaaattaat ggaaactagc
    13021 aataaagtat caataagaga gataataaaa gagttgaagt taaaagtagt atacatgcct
    13081 gatgatgtag attattatgt ctactctcaa gatataaata gaccaggatt acagtttgca
    13141 ggatattttg attattttgc atatgaaaga atacaaatag ttggaaagac agaatataat
    13201 ttttttggat taatggatag tcatattcgg gaagaagtac tagatagatt tttctcatat
    13261 gaaataccag cgttaataat ttctagagac ttggaaatca agccagatgt aattgaaaag
    13321 gctaaaaaat ataaaagaat acttttgagc acaaatagaa ataccactag acttataaat
    13381 agactgtcaa actatttgga taataagtta gcaccacaca caacaattca cggggtgcta
    13441 gtagatattt atggtatagg tgtactgata aaaggtgaaa gtagtatagg aaagtcggaa
    13501 acagcattag aattgattca aaggggtaat agattagttg cagatgatgc tgtagaaata
    13561 agaaaattag atgaaagtat gcttgtgggt cagtcacctg aactaataag acattttctt
    13621 gagataagag gaataggaat aatagatgtt agaagtttat atggagtagg ggcaatcaaa
    13681 aattctaaaa tgatagattt agtagttcat ttagaagctt gggatgaaac taagtattat
    13741 gatagactgg gactggataa agaatatgaa gaaatacttg gtgttaatgt agagaaatta
    13801 gttgtacctg ttaaacctgg aagaaatact gctatgatat tagaagtagc agcaatgaat
    13861 tttagacaaa gaggaatggg atacgatgca gctcaagaat ttactaaaaa attatcaaaa
    13921 ttaatcgata ctaagtagta ctgaataata gtgaactaca catcatttaa ttaaatattg
    13981 attaggatta tgtcctacac cttataaatt aaaatcaagt tgtatatata taatgtttat
    14041 taataagata tattatataa ggataattta aagtacagtg ttagattata ataatactag
    14101 attaatttta tatttgagtt tcattatata acaagaaagt tggaattttt tatgggaata
    14161 gaatatttta gtaaactttt agaaagtagt actgaaaaag aattgctctt aagtaaaaag
    14221 tcaaataaca taagtacagc aagattaata tcttttttga tagtgatagc agggtttgct
    14281 attggatttt acaataaaaa tatagtaggt atttttgttg gagttttatc aataataata
    14341 tttatagcac tcttagttat tcatagtaaa gtaaaagaag aagaaacata ttttaaaagt
    14401 aagagcgaag tattaaataa gtatattaag agatttggag atggttggaa ggaatttaaa
    14461 atagatggta aagaatactt aaaagatgaa aattcgcaag caaaagattt agacttattt
    14521 ggaagagcat cattgtatca atatatctgt gtagctaata catcttatgg gaaaaatttt
    14581 ttagccaaat atttgtggaa tgaaaatcca gatgagaatg taatcttaga aagacagaga
    14641 gctataaaag aacttttatc aaaagatgat ttttctattc atattcaaac actcagtaat
    14701 ataattggta aagagcaaaa aaacaattca gatagtagta tagaatcgtt tatagaatat
    14761 ggagaaaata agaatgtgta tataccaaaa tggatgcata tttttacttg gggtttacca
    14821 actgcaacaa tcttatcttt tatattttgt atgttaggat ttttacctgt tttacctgtt
    14881 tttttacttt ttataatcca gttagggttt agtggttttg gatatccaag acttatgcaa
    14941 actcttgctc cattattttc atttagtaga agtatacaag tatatgaaaa aatgtttgag
    15001 gttttagaaa aagagacgtt tgagagtgca tatttgaaag aattacaaga taaactttct
    15061 aagggaagtg gagtttcaag aggtgttaaa caattaaatt caataggaaa tgctgtaaac
    15121 ctaaggtata atcagatact atatataatt gcctgtggtg ttttgatgtg gaactatcat
    15181 tgtgcagaag ctttggagcg atggaaaggt atatatggaa atcaaattag agattggttt
    15241 gaatctattg gagaatttga agctctaata agtttaacag ttgtttctca tgtaaaggaa
    15301 aatacatgtt ttccagtaat aaaatatgaa gatactccaa gattgaaagt tgaagaggta
    15361 taccacccat taatagcaga aaaaagtgtc atagcaaatt cgatacaatt aaattctcag
    15421 acttgtatta tcacaggttc aaatatgtct ggaaagacaa catttttaag aagtatcggt
    15481 gtaaacttag tgctggcata tgcaggtgct ccagtatgcg ctaagagttt tgatgcgacc
    15541 tgtatggcta tatttacttc tatgagaata caagatgatg taagtcaagg aatatcaact
    15601 ttctatgctg agattcttag aataaaatca atgattcaat atagtttaaa agaactgcca
    15661 atgttggttc tagtagatga aatttttaag ggaactaact ctgctgatag aattattgga
    15721 gcaagtgagg cagtaaagaa attatcaaaa ccctgggtta taagtatggt gacaacacat
    15781 gactttgagc tatgtgacct ttcaggtagt ggagatgttg aagttgtaaa ctatcatttc
    15841 tcagagtact atgtagacga taaaattcat tttgactata agataaaaga tggaagatgt
    15901 aaaactacaa atgcaaaaca acttatgaga atggcaggta ttctttaatt aatagatagg
    15961 ttaattaaag aataagtcta tttagaaata taatttaatt tgtgaaatta ataaggattg
    16021 tcttaaatta cgtaaaagta ttttaagaca atcctttttt aagaataaat tttatatttt
    16081 gttatgacta cttagcttct tcacaagcac attcttgtgg acactctcca tagacacaac
    16141 tcttatcaaa gtaatgagta tgaaggtatt tatgggctac atcactattt ggttctccaa
    16201 agaagtcttt ataaatagct tgtaaatcag gattctcatg agatttacga atagccttat
    16261 ttctatctat ttcatataac gcatttgcac gtctatcaac tatttcagta ttaccatggt
    16321 gataaggttg accagcacct ccaacacatc caccaggaca tgccataact tctatgatat
    16381 ggtatttaca ttctccactt ctgaccttat ccataatctt tctagcgttt ttaagtgtat
    16441 ttacaataca cacattaact gtagttccat caacatctat acttgcttcc ctaattcctt
    16501 taagacctct aacattagtg aagtttacat tatctaaagt cttattagtt atcttctcat
    16561 atgaagtacg tagggcagct tctaaaactc caccacttgc tccaaatata gatgcagcac
    16621 ctgtagattt tcctagagga ttatcaaaat cttggtcttg aagattaggt aaatcgattg
    16681 cagcttcttt tatcatctta gctaactctc tagtagttat agaaaggtca acatctagta
    16741 ttccagattc acttaattct tctcttgaag cttcatattt tttagctaca caaggcatta
    16801 cagatacaac ataaagttct tcaggattga ttcctaatat tttaggtgca tagtagtttt
    16861 tagctataga accaaacata ccttgaggag acttacagct agatgctaag tttaacttgt
    16921 ctggataatt gtgctctaag aagtttaccc atgcaggaca acaacttgtt aatataggaa
    16981 gattttcacc tttttggatt ctttctataa attcagtagc ttcttccatt atagtaaaat
    17041 ctgcaccaaa gtttgtatca aatacatgtt caaatccaag tgcttttaaa gcagctacca
    17101 ttttccctgt agagattgac cctggttcta atccaaactc ttcaccaata gcaactctta
    17161 cagcaggagc aacttgcact actatagtct tttctttttt atttaaaaca tcccaaagtt
    17221 taggtacatt atctacttct gttaatgctc cagttggaca gacagatata cattgaccac
    17281 agaatgtaca ctctgtttca accatatcag agttaaagaa tgtactaact aaagtattaa
    17341 atccacgatt aactccagat aatgctccta ctgtttgtat gtcattacat acagtctcac
    17401 atcttctaca taatatacac ttagaatggt ctttaactaa agatttagta gaagtatctt
    17461 tacctgcaaa agatttagaa ccttgatatc ttatttttct tactcctaaa tcagcagcta
    17521 tagtctgtaa ttcacaatct ccattttttt cacagacaaa acaatcttgt ggatggtctg
    17581 ataacaataa ttcaacaata gttctacgag catttaatgc tttagcacta ttagtctgaa
    17641 ctttcatacc ttcctttata actgttccac aagctggaac taatcctctc tctgtctcaa
    17701 ccatacaaac acgacaagaa gcacaagtgt caagcttatc tatttcattc atatgtaaat
    17761 gacaaaggtt gtgtattttt atatttatta atttcgcagc atctaagata gaagttccac
    17821 taggtgctga aacgtgtttg ccattaatag ttaaattaac taaactcatt gttattcctc
    17881 ctccaaataa gttatttctt gattatagcg ttaaatgtac aattatctat acaagcacca
    17941 cattttagac acttagaagt atctattgta tgttgttcct taacagaacc tgttatacaa
    18001 ccagcaggac acactctaga acattttgta caacccttac agtcatctgt tataaagtaa
    18061 gaaagtaagt tctgacattt accagcagga catttcttat ctacaacatg agctatatac
    18121 tcatctttga aatattgaag agtacttaaa acagggtttg gagcagcttt accaagacca
    18181 cataatgaag cagtttgtat agtttcagca aggttttcta ggtcatctaa atcttgtaga
    18241 gtaccattac cttctgtaat tttagttaat atttctaata atcttgtagt acctatacga
    18301 caaggtgtac atttaccaca agattcttca acagtaaact caaggaagaa cttggctata
    18361 tctaccatac agtcagattc atcaagaaca agcataccac cagagcccat cattgaaccg
    18421 atagaactta atgaaccaaa gtctatagga gtgtctaaat gttcagttgg tatacatccg
    18481 ccagatggtc cacctgtttg aacagcctta aatttcttac cttcagggat tcccccacca
    18541 atttcaaata caatatctct taaagtagta cccattggaa cttcaacaag tcctacattt
    18601 tctacttttc caccaagagc aaagacttta gtaccactag aatcttcagt acctaagttg
    18661 gcaaaccaat ctccaccctt aagtataatt gcaggtatgt tagcaaaagt ctcaacgtta
    18721 tttaagcaag taggagattt ccatagacca ctcttagaag aactataagt tttcattcta
    18781 ggttcaccac gtctaccttc aattgaatgc attagcgctg taccttcacc acaaacgaaa
    18841 gcacctgcac catattttaa ttctaattta aagttaaatc cagtgcctaa gatattttcg
    18901 ccaagtaaac cgtatttttc agcttgagct atggctactt ttaatctttc tatagatttt
    18961 ggatattctg ctcttatgta tatgtatcct gtatcagaac ctatagcgta tccacatatt
    19021 gccatagctt ctagtacact atgagggtca ccttcaagta cagatctatc cataaatgca
    19081 cctgggtcac cttcatcagc attacataca acaaactttt tatctgttgg accagctgga
    19141 ttttttgaag cagcttccca tttagagcca gtagggaaac ctgcaccacc tctaccacga
    19201 aggccagaag tttttacctc tgcaataact tcttgaggag taagagaagt taaacatttg
    19261 cctagagcta aataaccatc attggcaata tattcttcta tactatctgg gtctataaga
    19321 ccacaatttt ttaaagctat acgtaactgt tttttgtaga aagacatttc ttcttgttct
    19381 tcaacttttt tacttaaaga ttcttcctca tataataatt cctcgacaac tgtatttcta
    19441 attaagtggc tttgaactat cttttcagca tcagatggct caacttttac gtagaaaacg
    19501 ttatcaggat aaacttttac aataggacct tgagcacaga aaccaaaaca acctgtcaaa
    19561 cgaacactaa ctttatcttg gattcctgat ttctttattt cattttctaa ttcactaact
    19621 atttctagac tgtttgaaga tgtacatcca gtatcacaac aaacaagtaa ttctcttctt
    19681 aatacatttt ccttagaata attttttctt aagctaagat taggtttcag ctcatcagct
    19741 aaaactttta attcatcaaa tgaatttact ttacgcatta tttattcacc cctcttaaca
    19801 attcaattct aattctctat aagtatctaa tatttcgctt acttggtcag gtttcacttt
    19861 accataaact ttaccattta cagtaacaac tggagctaaa ccacatgccc ccaaacatct
    19921 taaaccttct aaagaaaatt taaaatcact agtagtttct ccaactttta tgccaagctg
    19981 tttttcaaaa gcacttaaga ttttgtcagc accttttaca aaacatacag ttcctagaca
    20041 gacactgatt ttatacttac ctacaggttc tgtagtaaaa taagaataga atgtaacaac
    20101 cccatatacc tttgcaggag ctactccaag ttttctagca acatgaagtt gaacttcttt
    20161 tggaagatat ccaaatatac cttgggcttc atgtagaact tgtattaacg caccttcctt
    20221 tgttgcaaga gagtcaataa atatatctag ttcgtcaaat aattgcttgt tgtgtgaaat
    20281 aaaatcgcac ataacaaatc ccccttttct ttttaaagtt attatttttc ataaaataat
    20341 taaccatgag ttgaccccaa cattatgtgg gtgaacaaaa ccaaaacaat aaagaaagaa
    20401 taatatacaa caatttcttt atttataaaa acaatgcaaa aaagttacct
//
LOCUS       DAEMGG010000065        20306 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEMGG010000065 DAEMGG010000000
VERSION     DAEMGG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.065"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..757
                     /locus_tag="KPK24_002228"
     CDS             <1..757
                     /locus_tag="KPK24_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2299899.1"
                     /translation="MYCGQYIKLPKLGMVKIRDKQVPQGRILNATISKEPSGRYYVSL
                     CCTDVDIEVFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRK
                     TIGSLNRNKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRK
                     LSRLISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIRE
                     WICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            875..1180
                     /locus_tag="KPK24_002229"
                     /pseudo
     CDS             875..1180
                     /locus_tag="KPK24_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            1193..2098
                     /gene="truB"
                     /locus_tag="KPK24_002230"
     CDS             1193..2098
                     /gene="truB"
                     /locus_tag="KPK24_002230"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG2299900.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            2229..3158
                     /locus_tag="KPK24_002231"
     CDS             2229..3158
                     /locus_tag="KPK24_002231"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG2299901.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            3262..3519
                     /gene="rpsO"
                     /locus_tag="KPK24_002232"
     CDS             3262..3519
                     /gene="rpsO"
                     /locus_tag="KPK24_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG2299902.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            3759..4604
                     /locus_tag="KPK24_002233"
     CDS             3759..4604
                     /locus_tag="KPK24_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG2299903.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            4729..6840
                     /gene="pnp"
                     /locus_tag="KPK24_002234"
     CDS             4729..6840
                     /gene="pnp"
                     /locus_tag="KPK24_002234"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG2299904.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            7106..7849
                     /locus_tag="KPK24_002235"
     CDS             7106..7849
                     /locus_tag="KPK24_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2299905.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            8007..9254
                     /locus_tag="KPK24_002236"
     CDS             8007..9254
                     /locus_tag="KPK24_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG2299906.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGI
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            9289..9534
                     /locus_tag="KPK24_002237"
     CDS             9289..9534
                     /locus_tag="KPK24_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG2299907.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGDILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9544..10767
                     /gene="dapG"
                     /locus_tag="KPK24_002238"
     CDS             9544..10767
                     /gene="dapG"
                     /locus_tag="KPK24_002238"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG2299908.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10880..11614
                     /locus_tag="KPK24_002239"
     CDS             10880..11614
                     /locus_tag="KPK24_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG2299909.1"
                     /translation="MKDYREDNEKFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            11737..14148
                     /locus_tag="KPK24_002240"
     CDS             11737..14148
                     /locus_tag="KPK24_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG2299910.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            14151..15209
                     /gene="mnmH"
                     /locus_tag="KPK24_002241"
     CDS             14151..15209
                     /gene="mnmH"
                     /locus_tag="KPK24_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG2299911.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            15203..16537
                     /gene="rimO"
                     /locus_tag="KPK24_002242"
     CDS             15203..16537
                     /gene="rimO"
                     /locus_tag="KPK24_002242"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG2299912.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16524..17066
                     /gene="pgsA"
                     /locus_tag="KPK24_002243"
     CDS             16524..17066
                     /gene="pgsA"
                     /locus_tag="KPK24_002243"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG2299913.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            17354..18400
                     /gene="recA"
                     /locus_tag="KPK24_002244"
     CDS             17354..18400
                     /gene="recA"
                     /locus_tag="KPK24_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG2299914.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGISVSKEIVEE"
     gene            18576..20117
                     /gene="rny"
                     /locus_tag="KPK24_002245"
     CDS             18576..20117
                     /gene="rny"
                     /locus_tag="KPK24_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG2299915.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8307 a         2059 c         3927 g         6013 t
ORIGIN      
        1 tatgtattgt ggtcaatata taaaactacc taaacttggt atggttaaga taagggataa
       61 acaagtacct caaggtagaa tacttaatgc tactatatct aaagaaccaa gtggtagata
      121 ttatgtatca ttatgctgta ctgatgtaga tattgaagta tttgaaaata ctaataatca
      181 tataggttta gatttaggaa ttaaagaatt ttgtatctca agttgtggag actttataga
      241 aaatcctaag tatcttaaga agtcattaaa caagcttgct aaattacaaa gagaattatc
      301 gagaaaaaca atcggtagtt taaatagaaa taaagcaaga ttaaaagttg caggacttca
      361 agaacatata gctaatcaaa gaaatgattt tctacaaaaa ctatctacta aattaataaa
      421 agagaatgat attatttgca tagaagattt acaagtaaag aatatgatta gaaatcgtaa
      481 actatctcgc ttaatttcag atgtatcatg gtcagaattt attcgtcaat tagaatacaa
      541 ggctaattgg tatggtagaa aaattgtaaa ggtaggtaaa ttctttgcaa gttcacaaat
      601 atgcaataaa tgtgggtata aaaacgagga agttaaggat ttaaatataa gagaatggat
      661 ttgtcctagt tgtaatgaaa ctcacgatag agatataaat gcaagtataa atatattaaa
      721 agaaggatta agactaataa cgattcaaaa taaataactt atgtaagaac cgtaggaact
      781 acggggatag cctgtggaga attagtaaga catattttaa tatgcaaaat tctgtgaagc
      841 aggaaccctg tgactttagt catgggaggt tcagtcaaag gtatctagta tagttttaac
      901 tcaagacatg atgaatagaa ataatattag ctttaatgat gttgatggaa taacaacata
      961 tacaagagac ataaaaggta tagaagttgg aattttattt aaagagaaaa aacaaaatga
     1021 aattaaagtg agttttaggt caaaaaatta tgttgatgta agtgaaatag ccaagttatt
     1081 tggtggtggt ggtcatgtaa gggctgctgg atgtactatt agagatagta ttgataatgc
     1141 aaagaaaatg gtactagagg cagtgttaaa atcaatttaa ggtgacagta aaatgaataa
     1201 gattataagt atattgaaac ccacaggtat gacatctcat gatgttgtaa gtagagtgag
     1261 aaagatttta aatataaaaa aagttggtca tacaggtact cttgacccag atgcttcagg
     1321 tgttttacca atctgtatag ggaaagctac aaaagtatgt gaagttattt taaacaaaga
     1381 taaatcgtat atatgtgagc ttactttagg tataagtaca gatacttatg atgcttcagg
     1441 tgaaatttta aaaaaagttg atgattttaa atttagtaat gaagacatag aaagagcttt
     1501 tgacacacaa agaggagaga taaatcaact tcctccaatt tattcagctt taaaggtaaa
     1561 tggaaagaga atgtgtgatt tagtaagaag tggtagacaa tctgaaatta ctttaaaaac
     1621 aagaagagta aatataaaag acataaaaat actaagtata aaaggtaata aagtaatgtt
     1681 ttatgttgag tgttctaaag ggacttatgt gagaagcatt tgccatgata taggagaata
     1741 tctaggttgt ggagctcata tgtcgttttt gaatagaaca tcttctggta aatttgactt
     1801 agataattca ataacattag aagaattaga attattttat gaaaataaaa ctttagacaa
     1861 atacttatat gatatagatt atgtccttga ttcgtttaat tatgttgtat taaaccctaa
     1921 tgctataaaa tattatagta atggtggctc aatagatgat aaaaggtttt taaaaaataa
     1981 tttcgataaa gatgatgaat ttgtaagagt atacagtaca gataatttct taggattagg
     2041 aaaattatca aaacacaata atacaataag tgttaaaagt gataagatgt tcatataacc
     2101 aatgtattta aaaaaacaac aaaagagata gtactataat aacttctata gtctatctct
     2161 tttacttatg ctataatagt ttgtgaataa gttaagagta aattatattt tagcggggaa
     2221 catttattat ggtgattata aaatctataa aagaaatagc aaacataaaa gaaagtgtca
     2281 ttaccatagg aaattttgat ggtgtacaca aaggacatca agttcttata ggaaaaacag
     2341 ttgaacatgc aaaaaaagaa aatatacaga gtatcgtatt tacatttgca aatcatcctg
     2401 taaattactt tagaccaaac tcaacaaaaa atattataag caatgatgat aaaataaaat
     2461 tattagaaga tttgggaata gatatagttg taatcatacc atttgatgag tatatgacta
     2521 aaatacctgc aaaggatttc gtagatgaga tactagtaaa aaaattaaaa gctaaaaaga
     2581 tagtaattgg acatgacttt acatttgcaa gggcaaaaga aggcaatgtt aatctattaa
     2641 aaagcttaga acatgaattt ggttttgagg tagaagtaat aaaacccatc aaaatcaatg
     2701 atgtaagagt gagtagtact tatattagaa gtttagtatc ccagggtgat atggctaatg
     2761 tcaaggaata tttgggtaga aattacaagt tagaaggttg tgttatacat tctaaacacc
     2821 ttggaagaac tataggattc ccaacggcaa atattgacct taagaataat atgctcgttc
     2881 caaagagagg tatttatgct tctattgtac atattggaga tgagacttat tttggtgcta
     2941 caaatgtagg ctataaccca acagttaatg gaaaaacttt gtctattgaa acaaatattt
     3001 tagagtttga tagagatatt tatggggaaa atataattgt tgaatttttg gaaagaataa
     3061 gagatgaaag aaagtttaat tcaatagatg acttaaaaaa tcaattgtat aatgatacaa
     3121 attatgtgta tgaaaactat gtttgcaaaa acaagtaatg tatgataaaa tgattaagga
     3181 aaataaccat tactcagcat ttgaatactc caatcaatgc ttagtaatag gcgtttctaa
     3241 aaaaatacgg aggtaatcta aatgataaac aaatcagaaa taataaaaga atatggaaga
     3301 gctgaagggg atactggttc tccagaagta caaatagctt tattaacagc tagaataaac
     3361 gagttaaatg gacatttaaa aactcataag aaagaccacc attcaagaag aggactatta
     3421 aaaatggttg gtagaagaag aaacttatta gcttacttaa aggaaaaaga tcttgaagga
     3481 tatagagcat taatagctaa attaggatta agaaagtaat ttcattcttt ataaaaaagc
     3541 aggtatagtg tatacctgct tttttatagt taattcatta taaagtaaaa tagttaataa
     3601 taaaagtagt ttatatgtta taaaaaatta taaaatgaat aatattgggt aagaatatta
     3661 aaatagtata caaatacata atcaatttaa aaggaaagac aggataaata tagaaatttt
     3721 ataacaggat aattagttgg aataaggagg atattatttt gaaaataaag ttagtatgcg
     3781 atagtttgtg cgatattcca gatgaaatat cagaaaaaga ttatatagag atagttccac
     3841 ttacagttat ttttaatgat agggaatata tagaaggagt agatataaag aaagaagagt
     3901 tttataaaaa agtaaaagaa atcaagcaaa tacctaaaac ttcacaagct acatatatgg
     3961 agtttaaaga agtgtttgat aagttcataa cagaaggtta tcatataatc tgtatgacag
     4021 gggctgcaaa cgcatcagga acttttcaaa gtgctatgat agctaaaaat gatgtaaatg
     4081 aaaatgaaaa aatacatata tttgatacta gaaatttatc tctaggtagt ggacaatatg
     4141 ttataaaggc atgtgaactt cttgaagaag gactgggatt tgaagaaatt atagatgaat
     4201 tagaaaatac tcgcagtagt gtaagattgc tttttgcacc atatacactt gattttttaa
     4261 agcagagtgg aagagttcca gttgcaactg ctttgatagg aaatatgtta aatataaagc
     4321 ctatattttt ctttgataat ggggaagcta agttggtaaa taaggttaga ggaataaaga
     4381 atatagcaag taaattggtc gatataattt tagaaatgaa tgaagggtct ttagaaggaa
     4441 aaatcgtcac aataggttgt ggagataatt tacacgattg tgaaatatta aaagatgaag
     4501 taaataaaag aataaaagca agaagagtat tatttactcg aggaggagtt tctatttgct
     4561 ctcatacagg gccagatata ttggctataa gttgttctaa ttagtagtaa tataaggttt
     4621 ttgcatatat atgtgaagat aattttatat tgttaattta tagtattttt gataatatag
     4681 taaatagagt gatatacgtg ttttttaata agaggaggac aagcattaat gtttgaacat
     4741 aaaatattta aaatggattt tgcaggaaga gaactttctg tagaaatagg aaagatttgt
     4801 gaaatggcta gtggtagttg tatagttcga tacagtgata gtatggtaat ggtaaataca
     4861 actaaatcag ccaaacctag agacggaata gacttttttc ctttaagtgt ggattatgaa
     4921 gaaaaattat actctgtagg taagatacct ggaggttttt taaagagaga aggcaagcct
     4981 tcagagaaag ctatacttac atcaagattg atagatagac caataagacc tttattccca
     5041 aaaggattta gaaatgatgt tcaggtagtt gcaacagttc tatcagtaga ccaagattgt
     5101 acaccagata tagtagcaat gataggttct tcaatagccc tttctatatc agacatacca
     5161 tttaatggac caacaggttc agtatgtgtt ggccttgtag atggtgcatt tgttgtaaat
     5221 ccaaacgcag agcaaagaga aaaaagttca atgcatctag ttgtttcagg tacaaaggaa
     5281 gcaataatga tggttgaagc cggagctgat gaagttccag acgaggtaat gcttgatgct
     5341 attttatttg ctcaccaaga aataaaaaaa atagttgagt ttatagaagg tatagttgca
     5401 gaagttggaa aagaaaaaat gcctgttgaa ttataccatg caggagaaga aataactcaa
     5461 ttggttcgcg aatttgcaac tgataagatg aaaaaagcag ttcagacttt tgaaaagtta
     5521 gagagaatgg aaaatatgga tagagttaaa gaagaaactt tagctcactt tgaagaaact
     5581 ttagaagatt ttgaagattt tgttggagat atagaagaag tattacaaga tataataaaa
     5641 gaagaagtaa gaaaacttat agtacatgaa aatgtaagac cagataatag aaaacttgaa
     5701 gaaataagac ctatatggtg tgaaactggc atgataccta gagctcatgg ttcagccata
     5761 tttactagag gacaaactca agtacttaat gttgctactt taggtgcttt aggagatgtt
     5821 caaaaactag atgggttaga tgaagaagaa aacaagagat atatgcatca ttataatttc
     5881 cctgcatata gtgttggaga agctagacca tcaagaggac caggaagaag agaaatagga
     5941 catggtgctt tagcagaaag agctttatta ccagtaatac catctcaaga agaatttcct
     6001 tatgctatta gattagtttc tgaagtatta agttcaaatg gttctacatc tcaagcaagt
     6061 gtatgtggct caacattatc tttacttgat gcaggtgtac ctataaaaga tatggttgca
     6121 ggtatagcaa tgggacttat aaagcatgat ggtaaggttg cagtgctttc agatatacaa
     6181 ggtatggaag accatttagg cgatatggac tttaaagtag ctggaacaga gtatggtata
     6241 acagcaatac aaatggatat aaaaattgat ggtatagata aagaaattct acaacgtgct
     6301 ttaaaacaag ctaaagaggg tagaatccat atactaggag agatgagaaa aactatatct
     6361 cagccaaaac cagaactttc accatatgca cctaagattg ttaaaatgca aataaatcct
     6421 gacaagataa aagatgtaat aggacctggt ggaaagataa taactaagat aatagacgaa
     6481 actggagtta agatagatat tgaacaaact ggggaagtat ttatatcagg tattgagatt
     6541 gatatgatta agaaagctca agaacttata aataatatag tagttgaacc agaagtagga
     6601 aaaacttata aaggtaaggt aagtagaata atgaactttg gagcatttgt tgagatactt
     6661 ccaggaaaag aaggattact tcatatatct catatagctc atgaaagagt tgccaaagtt
     6721 gaagatgtat tgaatatagg ggatgaagtt gaagttaaag ttacagagat tgatgaaaaa
     6781 ggaagagtta atttatcaag aaaagttctt ttaccaaaac cagaacataa aaataaataa
     6841 cttattatat aattaaaagc aaagtcttag gattttgctt ttttaaatac aaaaaagtag
     6901 ataattttaa aatgatagtt atggatggct tatagttctt tttctgtcag gctttactct
     6961 tataaagaag ctatttaaaa gctgattaat agagagcttt attgatatat atagaatcac
     7021 ttaagttata tttagtgcat aaacagagct tatttttaat agtattaaat aagaaacaat
     7081 ttgtacataa ttagggggag ttttaatgat aatgatggta cttaataaga gtaagctcaa
     7141 aaaaatcata atactaattt tagtagtgtt attagtatta tcaggagggg taatggtatt
     7201 aaagttattt aacaacaagc cagtatttaa tttatttaat ggtgtagatt taccatatga
     7261 aagaggtaca aaggataaaa gtggatatgt tgcattaacc tgtaatatag accttggatg
     7321 ggaaacagag tacgtagaat ccattttaga aacacttaaa aaagaaaatg taaagattac
     7381 atttaatgta actggaaaat gggcagaaaa gaataaggat gaattattaa agataaaaaa
     7441 acaaggacat gaaataggta atcatggata caaacattta gattattcta cattatcata
     7501 tgaagacaat tatgagcaaa tagagacctc taaaaagata attgaagaaa ttataggtga
     7561 aaaaactaaa ttttttcaag caccagcagg ttcttttggt ccagaaacag taaaggctgc
     7621 taaagcatta gggtatactt ctatcaaatg ggatgccgat actatagatt ggaaatacaa
     7681 agaccaacca gaggtaatca ttgatagaat gaagaaaaaa gacattaaag atagtagtat
     7741 aattttaatg catccaacca acgcaacgac aaaatgtata gatgatatta tagctattgt
     7801 aagagaaaaa ggactaaaac caggaaaact tagtgatgta tttaagtaga caaagtataa
     7861 atatttggtt aagattgtat aagttgtagt tgaattaatt aaaagactga cttataatgt
     7921 aatacatata aaaaataaat ataattaaaa catagtgttt tcaataatcc aatatgaaac
     7981 aaagaataaa ttgttaggag aatgtaatgt ataagacaaa aatactggaa aatgggctta
     8041 caataattgg tgaagaaata ccatatctaa agtcaataac attaggtatt tggataaatg
     8101 caggctctag gatagaagaa gcacaggtga gtggaatttc tcattttata gagcatatga
     8161 tgtttaaagg aaccaaaaac agaacatcaa aagagattgc tagttcaata gataatttgg
     8221 gtggtcaaat aaatgcattt acaagtaaag aatgtacatg ttattatgta aaactaatag
     8281 atgagcatat agatacagga attgatgtat tgagtgatat gatactaaat tctaaatttg
     8341 ataaaaatga tatagataag gaaagactta taatattgga agaacttaag atgtatgaag
     8401 actctccaga tgacttatct tatgatttat tagtggaaaa tatatatgct aatgatggat
     8461 taggaatgaa tataattggg acaaaagaat ctctgtacaa tataactaga gaatccatgt
     8521 tagaatactt aaataagtat tatattccaa ataacgctgt tatttctata gctggaaact
     8581 ttaactttga tgatatggtg gagaaaatca aatcaaaatt tggacactgg gaaaagaaaa
     8641 atttaagtat agacataagt gaggctaagt ttaatccttg ctttatatca aaaaataaag
     8701 atactgaaca ggtgaactta gctatgtgcc taaaaggtat tccttttgaa aatgatgaag
     8761 aagtctattc aatggcagtg gtaaataata ttttcggtgg aagtataagc tcaaggcttt
     8821 ttcagaaaat aagggaagaa aaaggtttag tatattcaat atactcatca cagacattat
     8881 ataggaaatg tggtgaatta ggtatatttg caagcatgag tacagaaaat ttacaagatg
     8941 tatataattt aataaagaaa gaaattgaaa atataagaga aaactatctt acagaaaaag
     9001 aaatatctga aagcaaggaa cagttaaaag gaaattatat attggattta gaaagtacta
     9061 gcagtagaat gatgtctact ggtaaatcta tgttacttag caaaaaagta aaaactacag
     9121 atgaaatact tgaatgtata aataatgtta atattaattc aataaagaag gttgttgata
     9181 aggtattcaa tatcgaaaat attggtacat gtatagttgg aagagatgta gaaaagattc
     9241 tacacttgga ttaatattga aaaaagtaaa tctctggagg tataaaatat gaatttatct
     9301 gaaatagcag gtaaggaaat agttaacttg gtcacaggag aaagattggg ggtcattgga
     9361 gaatgtgacc ttatactaga tgaaactaca ggcgatatat tagcgcttct aattccaaaa
     9421 gaaaaaggat tttttgccag aagaaaagat aagtcatttt tagaagtacc atggagaaat
     9481 gtaaaaaaaa taggtaatga catgataatt atagaatatg agggatatat ttaggaggaa
     9541 aaaatgagta ttttagtgca aaagtttggt gggacatcag tagaatcata tgaaaaaatg
     9601 aatgaagtat gtaagatagt aaaagcatac aaaaaaaatg atgaagaatt acaattagta
     9661 ttagtagttt cagcaatggg aagaaaagga gctccatatg ctacagatac acttataaat
     9721 ttgtgtagtg cagtaaatga tgagccaagt aaaagagaac ttgatttaat aatgtcttgt
     9781 ggagaaataa tatcaggaac aattcttgct aatttattga atgcacaggg gatagattct
     9841 gttttcttta ctggtcagca agcaggtata attacaagtg atgaatattc taatgcaaag
     9901 ataaagtata taaatcctaa aaaggtaaaa agagctttag atgatggtaa ggttgtagtt
     9961 atagctgggt tccagggtgt aacagatgat ggagaaataa ctactcttgg aagaggcgga
    10021 agcgatacat cagcagttgc tattggtaaa gctttagaat gtgaaacagt tgaaatttac
    10081 actgatgtgg atggtataat gactgcagac cctagagtag aaccaaacgc taaagtattg
    10141 agttttatag actatgagga agtattccaa atggctgaca aaggtgcaaa agtaatacat
    10201 ccaagagcag tacagttggc taaaagtgga aatataactt tagccataaa gaatacaatg
    10261 aatccaactt ttgaaggtac aaaaataggt tctttatgta gacacttaga agacaatata
    10321 gaatatgaac aagaaagaga ttttaaggtt gctgttgcaa ataaagatag cgtagcacaa
    10381 gttaagatta agtcagcaga agaagtgttt acagaagtat taaatgagat agaaaagaaa
    10441 cttataacta tagatatgat aaatttcttt atatcagaaa aagcatttgt tgtagaagat
    10501 gctgatataa aatcattaaa ggaaatacta gataaatttg aattagatta tgaagtaaat
    10561 agagactgtg ctaaggtaac attgatatgt tcaagaattg ctgatgaaat gtctggtatc
    10621 atgtcaaagg tagttagagg attatcaaaa gcaggggtat ctcttttaca aacatcagac
    10681 tcaaatatga ctatatcttg tttagttagt gaagaagata tgcatacagc agtacatgct
    10741 atacaccaac aattttattt aaaataaact gtataataca attatttttt aatcaaattt
    10801 aagaatttca tatatatctt aatatatttg atttatttgg taaaactaaa ttaaatataa
    10861 ttttgatagg agttttttta tgaaagatta tagagaagat aatgaaaaat ttgataaaga
    10921 taattgtagt gaagaatcta gttgcgaaga gagagaatgt ataaagcaat ttggtaccaa
    10981 tgaaatgcca cctcaacctc ctaaagatat tcaatgtata actataattg gagaaattga
    11041 gggacatttt ataggaaatc ctcaaaaaaa agctacaaag tacgagcata taattccaat
    11101 gctatactca attgaggaaa gtaatgatgt taaaggagtg ttagttgttt taaacactgt
    11161 tggaggagat attgaagcag gtttagctat tgcagaactt ttaaacagta catctaaaaa
    11221 agtagttact cttgttctag gaggaagtca tagtataggt gttcctcttg ctacagcagg
    11281 agattactca tttatagcac caacagcaac catgattata catccagtgc gaactactgg
    11341 tcttgtgatt ggtataaatg agacttttga atattttaaa aagatgcaag atagaataat
    11401 tcaatttata ataagaacat caaatatcaa aaaagatgta cttgaaaaac ttatgcatga
    11461 aaaagatgaa cttgtaagtg atgttggtag tgtattaata ggaaaagaag ctgtggatta
    11521 tggtcttata gatgaagttg gaggactaaa agaagcactt aaaaaattaa gagagcttat
    11581 aaaagaatct gaggaagaaa aaaataatga ttaaaatttg tttaaataat aaaatttatg
    11641 atataatgat gtaataaagt aggagtgtta gtgttaaggc attagcactt tttgctgtga
    11701 aggggtaaaa cttgctaaaa ataaggagtg atattaatgg ccacgaaaaa gaagaaaaaa
    11761 aagaaggtaa ataccttaag ttttaatgca gaatatcata atttaataac aatatttata
    11821 ggtgtatttt tattatatag tttaaattct aattctatgg gatggatacc tgtgttaatg
    11881 caaaatcttt ttaagggact atttggaggg ctttcaatag caataccatt tatagtaatt
    11941 ataacaggac ttttaggatt ttttgatgga aatgaatata tatataggct aagaaagact
    12001 aaactatact acataattat tttatttata tttgtatttt atggattatt aaatgcagga
    12061 actcttcctg tagatagccc actaaaaggt aacatgtttg atgatgttat gaaattgggt
    12121 gtaagtggac aaggtagtgg tttgatagca acaactattg cgtattatat gagtaaaata
    12181 tttggtatag caggtggatg gttaataagt atcttcgcac ttatactttc tgtaatgttt
    12241 atatttaata tttctataaa agatttggtg tcaaatgcaa aatcaaaatc taaagcatct
    12301 aaagacagca atttaacgtt taaagataaa attgcaaata tgaaaaaatc tgcaatagat
    12361 atgatgactg atgaagtaga tgatactact ataaataaaa aaccaggttt ctttaaaggg
    12421 ttaatgtcaa aaggtcttaa taatgatgat gaagaagatg aatattttga agaagaaaat
    12481 agtgatggtg tagatgataa aactataaaa atagttggat ttaataaggc agaagatgag
    12541 tatttagaaa tattagaagg aactcaaagt atgccagagt tagatgtttt aaaagaatta
    12601 caaaaagcta ccaatgaaaa tccagttgta gatactaaac cagaaaaaaa agttgatata
    12661 gctaaaccta atttaaatat agaaaaaact caacctatga gtattgtagc agagccagtt
    12721 aatgaggatt attcaaacta taaaaagcct agcattgagc ttttaaataa agtaaataag
    12781 aagtctgatg aaaatggtaa aaagaaagtg ttgaaaaatg cttctctttt agaaaagaca
    12841 ctatcagatt ttggagtgga agcaaaaatt aatcaagtta cagttggacc tactataaca
    12901 agatatgaaa tacaaccaag tccaggagtc aaggtaagta aaattgtaaa tttaactgat
    12961 gatatagctt taagtttagc agctaaaagt ataagaattg aagccccaat tcctggtaaa
    13021 agtgcaattg gaatagaagt tccaaatgaa gaagctcaaa tggttggtgt gagagaagtt
    13081 ctagaaagtg aagaatttaa taattttaac tctcctcttg caatgggact tggaaaagac
    13141 gtagcaggaa aaattataat tggagatata ggtaagatgc cacacttact aatagctggt
    13201 tctacaggtt caggaaagag tgtgtgtgta aatacattaa taagtagtat tttatataag
    13261 gcaaatcctg atgaagtaaa attactatta atagacccaa aagtagttga acttgcaaat
    13321 tataatggaa taccacatct attgatacca gtagttactg accctaaaaa agctgcaaat
    13381 gctttaaatt gggcagttac agagatgaat agaaggtaca aattatttgc agatgctcaa
    13441 gtaaaagatg taacaagtta taatgagaaa gcggaagaaa aacttcctaa aatagtaata
    13501 ataatagatg agttagcaga tttaatgatg gcaagtgcaa atgatgtaga agattatatt
    13561 tgtagattag ctcaaatggc aagagctgct ggaatgcact tgattgtagc aactcaaaga
    13621 ccatctgttg acgttataac aggggtaata aaggctaata taccatctag aatagcattt
    13681 gcagtatcat ctcaaactga ttcaagaaca atccttgata tgggtggtgc agagaagctt
    13741 ctaggtaaag gtgatatgtt attctatcca cttggtgcag caaaaccagt tagacttcaa
    13801 ggggcattca tatcagagag tgagtcggaa aaagtaatcg attttgtaaa aagtcaggtt
    13861 aaagatggaa taaaatatga agaagatata attgaaacta tatctaaggt taatacttca
    13921 aagggtagtg atgaggatga gtttttgagt gaagcgatag aatttgtcgt ggaaagtggt
    13981 caagcatctg cctctatgct acagagaaga tttaaaatag gattcaatag agcagctaga
    14041 cttatagatt ctatggaaga aagaggaata attggagcaa gtgagggaag taaaccgaga
    14101 aaggtattaa taagcaaaca ggatttgcag aatttagaag gtgaatagaa ttgtcagtta
    14161 taaaaataga aaaagcactt aagctagata aagtaatatt tgtagatgtg agaactgaag
    14221 gagaatatga agaagaccat atattgaatg caatcaatat gcctcttttt aaaaataatg
    14281 aacataatga agttggaaca atttataaga tgcagggaaa acatgaagca atacaaaaag
    14341 gatttgacta tgtatcgtat aaattaaagg atatatattt acaagcagca gaacttgcat
    14401 taaattatga taatattgtc atatattgtg caagaggcgg aatgagaagt ggcagtatag
    14461 taaatttatt atcatcatta ggtgtaaatg tatatcaact tgaaggtgga tataaggctt
    14521 atagaaattt tgttttagag tatttaaggc atgtaatgga tacaaaaaac tttgtatctg
    14581 ttcatggatt gactggtaca ggaaagactg atttgttaca tttattagat gaaaaaggaa
    14641 tagacgtgct ggatttagaa ggcatagcta aaaacagtgg ttctaccttt ggttttataa
    14701 cttttgataa aaagccacca tctcaaaaga actttgaaac aaaactattt gaaagtatat
    14761 ttttttcgaa agagaattat atatttgtcg agagtgaaag caaacgagtt ggaagtgtta
    14821 cagttccaaa tgaaattttc gaggcaatga cacgagaagg acatcatata ttattagaat
    14881 gtgatattga aaatagagtt gatagacttt gtagagatta tatttatgat aaagatgaag
    14941 gtaatatatt gattttaaaa gaatgtataa acaagtttag aaaaagattg ggtaataaag
    15001 tagtagatga ttatattgat ttattagaat caggtgaata taaagaactt gtaaaaagat
    15061 atcttttaga atactacgac ccactttata tgcattcagt agagaaatat aagtatagta
    15121 agattataaa ttttaatgat acacaaaaag ctttagaaga tgttatagac ttatatgaga
    15181 gtatattgga aggagaaagt gaatgttaaa aatagcattg gaatcattag gatgctcaaa
    15241 aaatttagta gatgcagaaa taatgatggg aattttaaat aacaaaggct ataaattaat
    15301 tggagatttt gaagaagctg atgtaattat agtaaataca tgtgggttta tagagtcagc
    15361 taaacaagaa tctatagata cgataataaa ctttgcagag ttaaaaaaaa ctggcaattt
    15421 gaagttgttg atagtaacag ggtgtctagc ccaaagatat tctgaagaat taaaaactga
    15481 aattcctgaa atagatgcta tagtaggaac aggaagctat caaaatatag ataaaatact
    15541 taaagaatta agtgaaatgc atcaaattgt aagccttaat gatatagagt ttgtatttaa
    15601 tgaagattta ccaagatata tatctacacc tagctatatg gcctatttaa aaattggaga
    15661 aggatgttcc aataattgta cttattgtat aataccaaaa ctaagaggta aatatagaag
    15721 tagaaaattt gaagatataa ttaaagaagc taagaaatta gctgaatctg gagtaaaaga
    15781 acttgtggtt atagcacagg atacgacaaa gtatggtttt gatttatatg gaaaagaaag
    15841 attatcagaa cttttagaag aacttgcaaa aatagatgga tttaagtgga taagggttat
    15901 gtattcatat ccagaatcta taacagaaga attgatacaa gttataaaga aatatgataa
    15961 tatatgtagt tactttgata tgccaataca gcatgcaagt aacaatattt taaaattaat
    16021 gaatagaaaa actactaaag aagatatatt aaataaaatc aatctgataa gaagtaatat
    16081 accagatgct atacttagaa ctacaataat agtagggttt cctggtgaaa cagaagatga
    16141 cttcaaacaa cttgtggact ttgttgaaga ggttaaattt gatagacttg gtgcatttgc
    16201 atattcaaga gaagaagata caccagcaga tagattgcca aaccatatag atgaagaagt
    16261 aaaaatacaa agaagagata ctttaatgat gattcaacaa aaaatatctg aagaacttaa
    16321 tgacaaaaag ataggaaaaa cttatgaggt gttaatagaa gaacagatag aagataatgt
    16381 atatacagga agaacacaag gtgatgcaga agaaattgac agtatagtgt atgtaaaaag
    16441 tgtagacaat cttgaagtag gagaatttgt aagtgttcaa ataaatgatg caatggaata
    16501 cgatttaatg ggagatgttt tatatgaact tgcctaacaa gttaactttg tttagaatat
    16561 ttttaatacc agtatttgtt ttaattatgt tgctcaatgt accaaataaa tttttaatag
    16621 cttgtattat ttttataata gcttcaatta ctgatgcttt agatggaaaa atagctagaa
    16681 aatacaatct agtcactgac tttggaaaat ttatggaccc attagcagat aagttattgg
    16741 ttatttcagc acttacatgt atgatagaag accatcttgt aagtagttgg atggtaatta
    16801 taatagtggc tagagagctt acagttagca tattaagagc tatagctgct gctgatggaa
    16861 aagtaattgc tgctggaaac agtggaaaac ttaaaacgat aactcaaatg gtatctatag
    16921 tgtttttatt gttaggtgct cagtttgaaa atgtattgat attaaatatt ggtgaaattc
    16981 tcattcttat agctacacta cttacattat attctggatg ggaatatcta tataaaaata
    17041 aagagctttt tatgtcttct aagtaaaaaa ttatgcttta ataaataaaa actaatgatt
    17101 aataaataaa aaataacaac ttaaaatttt aaaaatgtaa aaaagggtat actattatag
    17161 tatacctctt ttcattttaa gaatattttc ttttgtgtat ataattatta ataatggttt
    17221 ataaataaaa tgtaatgaat aaaaatataa tcattagtag cgtttttaaa ttgactaggt
    17281 ttgatttatg ttatataatt aatatataac aagagaacaa atgtttgtag ataaatgaag
    17341 ggatggtata gagatgagtg tagatcaaga aaaattaaaa gcgttgaatg aagctttagg
    17401 taaaattgaa aaagattttg gtaaaggttc agtaatgaaa ttgggagaag caacatctat
    17461 gtctatagat gttatatcaa caggagcgat tggtttagac atagctattg gtataggagg
    17521 tctacctaga gggagaatag ttgaagtata tggtccagaa tcttctggta aaactactgt
    17581 tgcgcttagt tgtgtagcat cagctcaaaa agatggagga atagctgcat ttatagatgc
    17641 agaacatgca cttgacccag tatatgcaaa agctttgggt gtggatgttg ataacctaat
    17701 aatatctcaa ccagatacag gtgaacaggc tttagagata gcagaggcat tgataagaag
    17761 tggagcgata gatataatag taatagactc agtagcagca ttagttccaa aggctgaaat
    17821 agatggagat atgggtgatt ctcatgtagg tttacaagct agacttatgt cacaagcact
    17881 tagaaagtta actggttcaa ttaaaaaatc aaattgtgtt gctatattta taaaccagtt
    17941 aagagagaaa gtaggaataa tgtttggaaa cccggaaact actactggag gacgtgcact
    18001 aaaattctat tcatcagtta gattggatgt tagaaaaata gatacaataa aacaaggtga
    18061 taaagttata ggtagtagaa ctagagttaa agttgttaaa aacaaagtag caccaccatt
    18121 taagcaagct gaatttgata taatgtatgg agaaggaatt tcaaaaattg gagacctttt
    18181 agatatagct gctgatgtag atatagttaa aaaatcaggt tcatggtata gttacaatga
    18241 tactaaactt ggacaaggaa gagaaaatgt taaaaaattc ttggaagata atttagattt
    18301 aactactgaa atagatgaga aagttagagc attttacaat ttaaatgaag aacatgaaga
    18361 atcaggtatt tcagtatcaa aagaaattgt agaagaataa gtaaaaatac aaaaaaataa
    18421 ctagtaatta aagacatgcc acgctcccta ttagcttgac acaatgttga aaaaattata
    18481 aaattaagtg aggataaaat atctaaattt aattttaatt attaggaata ttttttaatc
    18541 atagtgatga cattttggga ttattaacga aggaggtgga tgtcatagat agtgtagtaa
    18601 tgattgtaat aggtgcagct gttggtatta tagctggata ttttgttaga aaaaatatat
    18661 ctgaagcaaa aattggacaa gctgaaaact tggccaaaga aataatagat aaagcacacc
    18721 acgactcaga aacagtacaa aaagaaaaat tactagaagc taaagaagag atccataagt
    18781 ggagaacaga agctgaaaga gaaaacagag aaagaagaac agaagtacaa aagtatgaaa
    18841 aaagagtagt acaaaaagaa gaagtcttag atagaaaatt acaaaattta gaatctaaag
    18901 aagtaaacct aagtgaaaaa ttaaaggtag tggaaaagaa agaagaagaa gttgaagtaa
    18961 taaaaactca acagttagaa aaattagaaa gtatatcagg aattacttct gataaggcca
    19021 aagaaatcat cttaaccaat gcggagagag atgtaagacg tgaaatgtct ataatgataa
    19081 aagagataga aagtcaagct aaagaagaag ctgataagaa atcaagagaa attataggat
    19141 atgcaattca aaaatgtgca gcagatcacg tagccgaaac tactgtaaca gtagtaagct
    19201 tgcctaatga tgaaatgaaa ggtagaataa taggtaggga aggtagaaat ataaggactt
    19261 tagaaacctt aacaggtata gaccttataa tagatgacac tccagaagcc gtaattttat
    19321 caggatttga tccaataaga agagaaattg caagaattgc tctagaaaaa ttaatagcag
    19381 atggaagaat acatccagct agaattgaag aaatggttga aaaggcgaga aaagaagttg
    19441 acaatattat aaaagaatat ggtgaacaag ctgcatttga aactagtgta catgggattc
    19501 atccggaatt aataagatta ttaggtagac ttaactatag aactagttat ggacaaaatg
    19561 tacttaaaca ttctatagaa gttgctcata tagctggtat aatggctgct gaaataggag
    19621 cagatattag attggctaag agggcaggtt tattacatga tataggtaag gctgtagacc
    19681 atgaaatgga aggtacacat gttgaaatag gtatggattt acttagacgt tataaagagt
    19741 ctaaagaagt tatacatgcc atgagtactc atcatggaga ttacgagcct caaacagtag
    19801 aggctgtatt agttacagca gcagatgcaa tatcagctgc tagacctggt gcgagaagag
    19861 aaactttaga agcatatata aagagacttg aaaaattaga agaaatagct aattcatatg
    19921 aaggtgtaga aaaatcattt gctatacaag ctggtagaga aataagaata atggtaaaac
    19981 cagaagctat aaatgatgaa gaaatacatc tattggctag agatatgact aagaaaatag
    20041 aggatgaact tgaatatcct ggacaaataa aagtaagcat aataagagaa acaagagcta
    20101 tagagtatgc gaagtaaaag aaataatatc taaataataa gaaaatattt ttagaaattt
    20161 aaatttgcca caagatatga taatatatct tgtggctttt aaattgttta agtttaattt
    20221 tatataatgc aaaaataatc taagtgctaa aagtgctact tacaaaagat taataaaata
    20281 aagctttata tatgtattat ttatat
//
LOCUS       DAEMGG010000066        19988 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEMGG010000066 DAEMGG010000000
VERSION     DAEMGG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.066"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            468..1259
                     /locus_tag="KPK24_002246"
     CDS             468..1259
                     /locus_tag="KPK24_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG2299916.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            1288..2508
                     /locus_tag="KPK24_002247"
     CDS             1288..2508
                     /locus_tag="KPK24_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG2299917.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2528..3223
                     /gene="pxpB"
                     /locus_tag="KPK24_002248"
     CDS             2528..3223
                     /gene="pxpB"
                     /locus_tag="KPK24_002248"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG2299918.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            3253..4239
                     /locus_tag="KPK24_002249"
     CDS             3253..4239
                     /locus_tag="KPK24_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG2299919.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4434..5000)
                     /locus_tag="KPK24_002250"
     CDS             complement(4434..5000)
                     /locus_tag="KPK24_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2299920.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5352..6419
                     /locus_tag="KPK24_002251"
     CDS             5352..6419
                     /locus_tag="KPK24_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG2299921.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6438..7598
                     /locus_tag="KPK24_002252"
     CDS             6438..7598
                     /locus_tag="KPK24_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG2299922.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPISMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7611..9707
                     /locus_tag="KPK24_002253"
     CDS             7611..9707
                     /locus_tag="KPK24_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG2299923.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9866..10210)
                     /locus_tag="KPK24_002254"
     CDS             complement(9866..10210)
                     /locus_tag="KPK24_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG2299924.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10502..11392
                     /locus_tag="KPK24_002255"
     CDS             10502..11392
                     /locus_tag="KPK24_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG2299925.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11610..12896
                     /locus_tag="KPK24_002256"
     CDS             11610..12896
                     /locus_tag="KPK24_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG2299926.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12985..13428)
                     /locus_tag="KPK24_002257"
     CDS             complement(12985..13428)
                     /locus_tag="KPK24_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG2299927.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13452..14246)
                     /locus_tag="KPK24_002258"
     CDS             complement(13452..14246)
                     /locus_tag="KPK24_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG2299928.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(14812..15288)
                     /locus_tag="KPK24_002259"
     CDS             complement(14812..15288)
                     /locus_tag="KPK24_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2299929.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(15389..16558)
                     /locus_tag="KPK24_002260"
     CDS             complement(15389..16558)
                     /locus_tag="KPK24_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2299930.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTNTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            16721..17584
                     /locus_tag="KPK24_002261"
     CDS             16721..17584
                     /locus_tag="KPK24_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG2299931.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWGKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17655..18248
                     /locus_tag="KPK24_002262"
     CDS             17655..18248
                     /locus_tag="KPK24_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG2299932.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(18499..19134)
                     /locus_tag="KPK24_002263"
     CDS             complement(18499..19134)
                     /locus_tag="KPK24_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2299933.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(19214..19945)
                     /locus_tag="KPK24_002264"
     CDS             complement(19214..19945)
                     /locus_tag="KPK24_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG2299934.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEGDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT         7616 a         2333 c         3324 g         6715 t
ORIGIN      
        1 tattttaaca tgcatttagt atacatgatt ttattatact taaaactata taaagaatta
       61 attataaaaa tataaagata ttttacttac aaagaatgat aattatatag atgaaagaaa
      121 caaatgaatg aagaaaaatg attctgtctt gcatataaag tgattaaaaa tgaaaaatac
      181 tttaaaaata aaggttaaag cccttatttt aagtgaaaat aattaattat taactaaaaa
      241 atggtaaaaa gattttttga aaaagaaata caaatgaatg ttcaattaaa ataattgcaa
      301 aaatattcct taaatggtaa aattatatta attaaatgca atagaatagt tgcaaaaata
      361 aaattgaata caagtgataa aatatttcat gcaatatata aaataaacaa aatttaaaaa
      421 ttaaaactac ttattctatc aaaatgttag caaggagggg tcataaattg tataaagtag
      481 atttaaatag tgatttagga gaaagttttg gaacgtataa gattggttta gatgaagaag
      541 ttttaaaata tatttcatca gctaatatag cttgtgggtt tcatgcaggt gaccctagtc
      601 atatggaaaa aacagtacag ctagctaaaa aaaatggagt gaaaataggc gcacatccag
      661 gttttctaga tttaatagga tttggaagac gtgagatgaa aataacaaag caggaagcta
      721 aagattatac aaaatatcaa ttgggtgctt taatggcatt tgcaagttca aatggatgta
      781 atatacaaca tgtaaaacca catggagcat tatacaatat ggcagctaaa gataaagagt
      841 tagctatggg gatttgtgaa gctatatatg aagtcgataa agatattata ctgttggggc
      901 tatataatag tgaaatgata aattctgcta aagaaattgg acttaggttt gcaaatgaag
      961 tatttgcaga tagagcttat gataataatg gttttttggt tccaagaaat gtagaaggag
     1021 cagttataca tgatacaaaa catgcaatag atagggttgt taggatggta aaagaaggaa
     1081 ctgtagaaac tttaacaggt gaagttatac atatcaaagc agattctata tgtgtacatg
     1141 gggacaatcc aaaagcaata gagtttgtca aagaaataag aaagcgtttt gaattagaaa
     1201 gtatagaagt ttgtccatta gaaaacatag aagtttgttc gtcagagaac atagtataag
     1261 actgataaca tgagggggag ataaaatatg agtacaaaaa atattacaga taaaactaaa
     1321 aataaaaaag atgtcggagc acttataggg gctgcattta taatggcaac atcagcaatt
     1381 ggaccagggt ttttaactca gactgcacaa tttacccaag attttggacc gaatttttca
     1441 tttgtaattt taattacaac aatccttttt ataggggctc aagtaaatgt atggagagta
     1501 attggtgtat caggacttag aggtcaggat atagcaaata aaataatacc aggattaggt
     1561 tatttagtag cttttttagt agctcttgga ggattagctt ttaatatagg aaatgttggt
     1621 ggagctgcac ttggtatgaa tgtaatgttt aatatgaata tgacattagg aactgtatta
     1681 agtggattaa tagccatatt tgtatttatg tcaaaaaatt caaattctct agtcgataag
     1741 ataactaaat ttttagcatt aggaatgata attatagttg gatatgtagc aatatcaaat
     1801 catcctcctg ttggagaggc agtatcaaga atggtaaaac cagaaaatcc aaaaggactg
     1861 atattcccaa taataactct acttggagga agtgttggag gttatataac ttttgctgga
     1921 ggacatagat taatagacgg tggaataaca ggagaagaaa atataaaaga aataactaaa
     1981 tcttctcttt tgggtatatt agttgccaca atgatgagag ttcttttatt tttagcaata
     2041 ctggcagtgg tttcaaaagg tcttcaactt gacccagaaa atccagctgc ttcagctttt
     2101 aaatttagtg caggagcaat aggatataaa ttctttggac ttgtattatg gtctgctgca
     2161 attacatcag ttataggagc tgcttataca tcagtttctt tcttaaagac actaaatcct
     2221 tttatagata aatatgaaaa atattttata atagcattta tagcaatatc aacacttata
     2281 atggcattta taggaaaacc agctacttta ttaattttag caggagcttt aaatggattg
     2341 atacttccaa tcacattagg aattatgctt atagcatcta aaagaaaaga tattgttgga
     2401 gattacaagc atcctacatg gcttttaata tttggattaa tagtagtttt aatatctgca
     2461 tatacaggaa taacttctct tagcagccta ggtgctttat ttgcttaatt tagaagaggt
     2521 gacatttatg gaaactagat acttgatatc aggggataag gcagtggttg ctgaatttgg
     2581 aaatgagata tcagaagata taaacaaaaa agtaatttca tttatgagag caatagaaat
     2641 atcgaatctt aaaggtattg taacagaaat ggtaccaaca tataggtcac ttatgataag
     2701 ttataatcca ttagaaatag attttgatag tttgattgag aatttaaaaa aaatagaaga
     2761 taatttggaa agtatagtat taccaaaacc taaaatacat gagattccag tatgttatga
     2821 tgaggttttt ggtatagata ttaaaaacgt tgcaagctac aataatctca ctgtagatga
     2881 agttataaaa atacacacaa gcagagagta cttgatatat atgctaggtt ttactcctgg
     2941 atttccatat ctaggtggta tggatgaaag aatagctaca cctagattag aagtgccaag
     3001 gactaaaatt tatggtggga gtgttggaat agcaggttcc cagactggtg tatatcctat
     3061 tgatagtcct ggtggatggc aaataattgg taggacacct ttaaaattat atgatgaaaa
     3121 tagagaagaa cagattttgc ttagagctgg agattttata aaatttgttc caataacatt
     3181 agatgaattt atagaaatcg aaaaaaataa cttaagtatt taaatatcgt tatagtacag
     3241 ggagtggtat atatgggatt caaagttgaa ttaggaggtt tccaaacatt aatacaagac
     3301 agaggtagag ttggctatgg tcaatatggt gtatctggat gtggagcaat ggatgagtat
     3361 gcacatagag taggaaatat attagtagga aattctgaag atgaggcttc tttagaagtt
     3421 ttaatgttag gtcctacaat tacctttgat gaatatacgc aaatagcagt aactggggga
     3481 gatttagggg ctaaaataaa tggaaaagaa attcaaaact ggaggtcata tcaaataaat
     3541 ccaggtgatg tattatcatt tagaggtgtg aaatcaggtg ctagagccta tgtgtcaata
     3601 gctggtggaa tagatgttcc acttgcaatg ggaagtaagt caacttatac aagagctaaa
     3661 attggtggat ttgaaggtag agcacttaaa aaaggagatt atataaatac ttttattcag
     3721 gaaaaagatt ttacaataaa taaaaaatta agttcaaaat atattccaac ttatagttca
     3781 gaaatcgtac taagaattgt aaaaggtcct caatttgatg ctttttctaa cggtgaagta
     3841 gagaaatttt tatcaaataa atacaaagtt acgaatgaaa tagatagaat gggttgtaga
     3901 ctagatggag agagtataaa gcaccttaat ggagcagata ttatatctga tggtatttct
     3961 tatggagcta tacaagtacc tggtcatgga aaaccgatta taatgttatc agatagacag
     4021 actagtggtg gctatactaa gatagggaac gttataagtg tagatttgta taagttagca
     4081 caagcaaagc caaatgatgt agtaaaattt gaacttgtag atatatatga agcacataga
     4141 ctattgagag agcaagaaga taagattcaa gatatctaca agagtatgaa aaatataaga
     4201 gttgtaaagg caaaagtatt aaatgatata gcagtttaat tttaacaaat acaattttta
     4261 ttataattta taatataact tacaaatagc tatttactat ttaccataat taagatggtt
     4321 tagattagtc aaatagctat ttttttagtt tgagtaaata ttttaaattt aaaataaaag
     4381 caatatttaa atctattata ttatctacaa aaagtaggtg tgtcatattg agtttattta
     4441 gattttattg gccaaattgg aggtgatata actccatatc cacgagctat tgtatcttta
     4501 cttggattga tgattttatg tggtacatta gcttttataa atgttgagtc acctggattt
     4561 aatctaacta ccttttcacc catacaaata tcaaccacac cactcgttac caccataatt
     4621 tcttctgatg catgatatat ataatctttg gcatcctcac tttctggttt aagttcaaac
     4681 tctacaaata aaattgaagg tgtaaactca gttgtaggca ttggagatac tatctcgtaa
     4741 aacatttcac tctttggaaa tttcatcata actctatctt cttttctaat gactaaatca
     4801 tcagtattat tgtcatccat aaacatatat aaaggagtgt ctaaaacttc agcgattttt
     4861 ctcaatgaag acaaggatgg ttcaatagca tctctttcta tttgagagat ataacttata
     4921 gataaatctg tcaatttgga aacatcactt aatgtttttt tcttgctttt tcttatattt
     4981 ctcatctttt cacctagcat aagaacctcc aaataataaa attaatataa aatattcgct
     5041 taaaacaaag taattatagc acaacataga agtagtggca ataagaatac atagtagatt
     5101 ttgacaatta aaatttaatt aaatccaaat aaaagtctat gaaaatgaat tattgttaaa
     5161 ttaatacaaa gaaaataata ataaaaaaat ttaatagaag agtattaatt gaattaagat
     5221 tatcacatta tattttggaa aagagtaaat tttcagtaaa ttatcttgac atatatattg
     5281 aagtaataat ataatttaaa gtataaaatt ttaaaaattg aattataact tcaaaaaata
     5341 aggagggtaa tatgaagata acagatatca aatttgaaaa gcttagaatt aaattaaaaa
     5401 aacctgtagt agtttcattt ggagtaattg agtatggaga aagtataatt ttaaaaattg
     5461 aaactgatga agggtattgt ggatttggcg aagcagcacc tcttgcagca gttacaggtg
     5521 aagttttgga taatgttctt tcagtattgc ttatgtttaa aaaagagtta ataggcaaag
     5581 acccactaga tattgaaacc atacatacca tcatggatgg aattattata ggtaatactt
     5641 ctgcaaaagc agctattgac atagctttat atgatattaa aggaaaaatt atgaatgcac
     5701 ctctttataa agttcttgga ggatttgata gtaaagtcca aacggatatt actataagta
     5761 tagataagcc agaaaaaatg gcagtagaag ctttagaaag agtaaaagaa ggttttagga
     5821 tattgaagct taaagcaggt ataaatcctg aagacgatat tgaagctgta aagttaatta
     5881 gagaagccgt tggagagagt ataagaatta gaattgatgc aaatcaagga tggaacgtaa
     5941 atagcagtat aaatactatc aaaaagttag aagaatttaa tgttgatgca attgaacaag
     6001 cacttccaca ttgggattta gatggtactg cttacataag aaataaaagt aacacaaaaa
     6061 ttatgataga tgaatcatta cattcaccaa tagatgcaat taaggctata aaaaagaatg
     6121 cagttgatac atttaatatt aagctgatga aatcaagtgg aatatatcct gctataaaaa
     6181 tcaacaacat agctgaggca agtggagtaa attgtatgtt aggatgtatg ctggaaacga
     6241 gaattggtat aacagcagca gcaaatttaa tagcatctaa aaagaacata acagaagctg
     6301 accttgatag ttttatgttt tgtgaagaat tagaaggtat atctggagga tttgtaatgg
     6361 atggagatat tatgaatctt gtcaataaac caggtttagg aatagaagta aacttatgat
     6421 aaatttggag gttaaatatg aatacacttt tttcaaatcc aggtagtata cttgctgtaa
     6481 tgacatcaat gattgcattg ggtttttact tacaaagata caaagccatt aaaagcttag
     6541 gaccagcact tacaataatc ataatgggaa taatactttc aaacttaaaa gtagttccag
     6601 taagtactga gttatatgga actatttcaa catatgctat tccaatttct atgactataa
     6661 tgttgatgag tgttgactta aaagaaatga caaaattatc aagagaacct ttaattgcaa
     6721 tttttgtagc tgtattaact gtaagtatca tggcattttt atttggatta gtatttgcag
     6781 aaaaaatatc agaaggttgg aaggttgctg gaatgtttgt aggtacatat actggtggaa
     6841 gtgctaatct tacagctatt ggaacaggtc ttaatgtaag tagacaaact cttgcagcag
     6901 caaatgcagc agactatgta attggagtac caacattgat atttatgttt gctctaccag
     6961 caatattaaa aaattcaaag aagtttaaaa aactttggcc atatcatgta gaagaatcag
     7021 aattagaaga ttgtcaaaat gaagaattta tggagtcaaa agaatggagt attaaagata
     7081 tagcttggat gttggctata ggttttgttg ttacagaagt ttcaacaata cttgctggat
     7141 atttcaattc aagttttagt agtgcagcaa gaatactttt agttacaaca atttcaatta
     7201 taattgctca attaaagcca gtaaagaaac taaagggaaa tttagattta ggattatttg
     7261 ttgcattatt ctttttatgt acaataggtt tttcagtaga tataaaagag tttttaggtt
     7321 caacttttac tataacactt tactgtttta gtataatttt tgcttcattt gttttccatt
     7381 taggcataac tagactttta aagataaagt atcaatatgt aatactttct atagtaggag
     7441 caattgctga tggtcctact tcagctttag tagctgcttc tgcaaagtgg aattcgcttg
     7501 taagtgtagc agtcgtaatg ggagtaattg gtggagtatt aggaaattat gcaggtatat
     7561 cagtggcata tgcaataaag atgtctctag gattataggg gggagtttaa ttgataaaat
     7621 ttaaattata catatctgga ctatacagtg gaaatgtaat ctttgatgga gatttattaa
     7681 ttgaaaagtt gaatcctttt acaaataaaa tagaaagttt aaaaccaata agcaaagaag
     7741 aaaatacata ttatttaaat cttacaaaaa tagatttaaa gtctttattt aataattttg
     7801 atgtatatac taaaagctta gtaaacacag ataaagacac agttataaat aacttaggag
     7861 aaacgcattc taaacttaat gaatatatat gggttcaaag aaacaaaaag ttcccactag
     7921 atataattat tgtagataat aaaatagtag gttttatctg tttatctaga gaaacttgta
     7981 ctatattaat tatggatgga tatgaagagt atactgtatt aaaagaatgg gaaaaaactc
     8041 ataaaaatga agagatatat tctataagat ttggtggaaa ttatatgatt gatatgaaag
     8101 atggtataaa actttctaca gatgtatatc tccctgattt tgtagattct gccaaaaaag
     8161 caccgacaat tcttatgaga actccatatg gaaaagaaaa tgataaagaa atatattata
     8221 aatatgtaca aagaggatat gctgttgtaa tacaagatgt taggggaaga aatgaatcag
     8281 aaggtaagtg ggaacctatg attcatgaga gggaagatgg agatagtacc ataaattgga
     8341 tagtatcaca ggaatggtca agtggaatag ttggaatgct tggagcatca tatcttggtt
     8401 atgtccaatg ggcagctgca tcttcaggca ataagcatct taaagcttta gttagtatag
     8461 taacatcagg aagccctttt attgatatac caagaaaagg aggagctttt gtatctggta
     8521 tgcttgcatg ggcatttatg gtatcaagaa ataaagttga tagaagcaaa atggtaagag
     8581 atgattggga tgatgtttta aacatacgac caatagaaaa tatacctgtt gaagctcttg
     8641 gatatagaat tgaatttctg gaagaatggt taaagagagt agaaaaagat gagtattggg
     8701 atttgatgga ctggcattta caaaaagata aaataaatgt acctgcattg gttgtatctg
     8761 ggtggtatga tgataatagt atgggaacta cagaggcctt agatgttata aaggactatg
     8821 aaaaaggaaa gagaaaggct attctaggtc catggatgca taattcaaat actttaagag
     8881 atataaatgg tatttcttta ggaaatagtt cattaagata tgatttggat tataattatc
     8941 tattatggtt tgataaatac ttaaaaggta tagaaaataa catagatacg actgctccag
     9001 tagagtatta ctctgtaggt tctaataagt ggaagacaga agaaaattgg ccaataataa
     9061 ataagataga taaaagtatg tatctaataa gtgatggaaa tgctaacaca tctttaggaa
     9121 atggtagatt agtatttgat aatgatttag aagaaaagta tgatagttat atatataatc
     9181 caaaagaccc atcagtacaa ctaattgata tgtcagagaa tgaagttgga gttccaaata
     9241 actacaaaga tttagaaaaa agaagtgata tgttgtgtta tacatcagat gcttttagtg
     9301 aagaatttac tgtaacaggt gatataaagt tagagttttt tgcatcaagt tctgccaaag
     9361 atactgactg ggttataaaa attatggacg tggatttaga tggaaattca attaaattag
     9421 ctgatggtat acttagtgct agatttagaa atagttttta taagtcagag tttatggaag
     9481 aaggagagat ttataagttt acgattataa cttcaaaaat atcaaacacg tttaaagttg
     9541 gtcataaaat tagactagat ataacgtcta gtgctaaaaa ctttattttc caaaatagca
     9601 acacgacaga gggatataat agtattgaat atatagaagc aaagaatacg atttaccatg
     9661 gaggtaaata tccatctaaa ttgatattgc caatagagaa taagtaaatt tatatagatt
     9721 aaatagtgag gaggtgtctc aaaatgaatt ttttagttcg tgagacactt tcttttatac
     9781 gaaatcaaat atattttcat catttcaaca atgaaaaaga ggcttatctc tctgttttga
     9841 gatagcctcc tataagctaa attgattatt ttgtagctgc aacaacgctc atttcaacta
     9901 acaaatgctc tctagcaagt ctagcttcaa cacaagctct agcaggttca aatccttttt
     9961 caacccaagc atcccaaaca gagttcatag cttcaaagtc tttcatatct cttaaataaa
    10021 tagtaacaga tagcaaatgt tgcttatctg aaccatattt gtttaataaa tcttctattt
    10081 tagctaaaac ttcagttgtt tgttcaatta ctcctccttc agcatgagtt tgtccacata
    10141 aataaactgt attgttatga actacagctc tactcattct tccagttcct tcatatctct
    10201 ttatttccat attttaaata cctccatagt taaaaaatct attaaaaatc taaataataa
    10261 attaaaaaaa gtctatacta tataaaatta tattatatat tctgataata atccataaaa
    10321 acatataact ttttagctat aattattatt gttattaatt gaaaagattt attagttact
    10381 tgacatagtt tacactttta attatagtat aaattggaat atttagataa ttattattta
    10441 atgtataagc taagttttta attaatatgg ctttatacat ttatatagaa aagaggtacg
    10501 tatgagtagc acaatcaaat caaatcgaat aaaaggtata atgtttataa tagcatcagc
    10561 gcttggcttt gcaatgatgt ctgcattcgt aaagctagct ggagatttac caagttttca
    10621 aaaagtgttc tttagaaatc tggtatcagc tataatagct ttttggctta tcataaaaca
    10681 taaaggaagt ctaactggta aaaaagaaaa tttaaaagta cttttatata ggtcaatatt
    10741 tggaactcta ggagttatat ttaactacta tgcaattgac aagttgatgc tttcagatgc
    10801 taatatgcta aataaaatga gtcccttcct tgtagtaata ttttgtgcaa ttgctctaaa
    10861 agaaaaaata aatgtaaagc agataggagc tattataatt gcatttatag gagctttatt
    10921 cataataaaa ccaacattta gtgtagaagt aattccatat cttggaggaa ttgcaggagc
    10981 tgtatttgca gcaatggcat atacttgttt aagagttctt ggagatagag aagattacta
    11041 tacaattgta tttttctttt cagttttttc attagttact gtagggccaa ttgcttttgc
    11101 agtatatgaa ccaatgaccc ttatgcaact tatatatcta ttactaggtg gagtatttgc
    11161 aagtctaggt caatttggaa taactctagc atataaatat gcacctgcta aggaaatctc
    11221 tatatttgac tatagtaata taatcttttc agcaatttta agtatattct tattcaatgt
    11281 ctatccagat atgttaagtg ttgttggata tttaatagta ttttcggcag cattttatat
    11341 gtttttatac aataagaagt tagataaatt agacaaagaa aagaagaagt aaactagata
    11401 aaagtgtttt tataatttgt atttaatatt taagtctttg tattaaaatt aaagaaaatt
    11461 taattaaaaa tgtttggtat attaacttat aacagcagtt aaaattgaaa aattataact
    11521 gctgtttctt ttattaaaaa tatccaaatg tctgtaaaaa tagactataa tagagtagta
    11581 taaataatat aaaggggata aattaaagta tggaaaaagt gaaagttgct gtgattggag
    11641 caggtaatag aggaacgtat gcatatgctc catatattta tgaaaattct gatgtatgtg
    11701 aaatagttgc tgtagcagaa cctaaaaaag gaagacgaga actatttaca caaaaatata
    11761 acctagatag taaaaatgta tttgaaactt tagaagactt ttttaaacat gacaagatgg
    11821 cagatgcagt aataatagct acaaatgatg atagacatta tgatgtagca aaactagctc
    11881 ttgaaaaagg ttatcatgta ctattagaaa agccaatgtc taatagttta gatggtctgg
    11941 tacatataga tgacttgtgt gataagtata aagataagat ttttatgatt tgccatgtac
    12001 tgagatattc tccattttat aataaattaa aagaaattgt agaaagcaaa aaattaggag
    12061 agctagttag tatacaatat aatgaaaata taggatattg gcattttgca catagcttta
    12121 ctagagggaa ttggagaaat agcaatgaaa ccagcccttt aattttagca aagagttgtc
    12181 atgacatgga tattttgtta tatttagtag gaagtagatg caaaaaaata tctgcatttg
    12241 gaagcttaaa gcatcttaat aaccaaaatg ctagtggaga gatggcacaa aattgtcttc
    12301 agtgtttagt tgaaaaaaaa tgtccatatt cagcaaagag aatttattta gaaaaagata
    12361 gaagtataaa tagagctgtt cacataaatc caactgaaga gaatctattg aatatactta
    12421 agacaagtcc atatggaaga tgtgtttaca gatgtgataa taatgttgta gacaatatgg
    12481 taaatatact acagtttgaa aatggagtga cggctacttt taatttatgt gcatttacta
    12541 aagaaaatgg aagaactata aagttaatgt ttagccatgg tgaagttggt ggggatttaa
    12601 ataaaaatga gataagaatt aaagaatttg gaaaaaacga agaaatagtt atgaatccat
    12661 caaatcaaca gaatatggta gaggaatatg atagaaatct tatagctgaa tttattaaat
    12721 tagtttcaaa taaagaacta gaaaagggta gagtagcagc aaaagaagct atacaaagtc
    12781 atgtaatggc atttgcagca gaatattcta gggtcagtga tgaagtagtg tatatagaag
    12841 aattttttga tagtgctaag caaatgacta aggagataga agaaactata ttttaattaa
    12901 taaaaattta ttaaataaaa taaagataga ttaaataata gaaatacgaa aatatgtgtt
    12961 ctattaagga atctatcttt tttattaagg ttttttctta tccctatcaa aaaaatccat
    13021 acattctgat aaaagataag aaagtaaagc gaataagatt tctgtactta aaggaatcgt
    13081 tactgaatcc ataaaggtat caattatgtt catagctaga aaagttagta taaaatcaat
    13141 tataaattcg caaagtttat atattgagta tggtaatttg tttacatatc tgaatatatc
    13201 taaaatagta gtgcatagaa aatcaattgg agtggttata caaaaatata ttaaaaagaa
    13261 aattaataca gctccaagag atttatattc aaatcctaag aatgataaga agccaattcc
    13321 tataaccgtg aaaatacaaa aagttatacc aaaaaaaaca gcaaaaaata aaaatacaga
    13381 aataaattcg cctaaaggaa tccttctttt attacttatc ttaatcaact ccttaattta
    13441 tgatatgaaa tttatataaa taataacatt cttaaaaatt cataatagtc ctctgtttca
    13501 aatagtactg tttgagaatc tatttttttc atatttggat aaaaagaggc cttaaaagct
    13561 ttataatgtt ctctaaattt tatttctatc ttaaatttat ctggcaattt tattaagtgc
    13621 ctagataaat ctccagataa agcttttttg acaccttttt caattttttt tagtgcaagg
    13681 tttggatgga ttgaaattga accatttcca attccttttg acacaggaac agtgactata
    13741 tttggattta gtttttttgc actctcacaa agcatttcat ctccacttaa aaatgctaca
    13801 ggaacattat aatatgcagc aatataagca tttatagtaa attcagatgc tatttcatca
    13861 tttattttaa aatagtcata atcaatatcc atagtatgag aaagtggatt accattacaa
    13921 gtagctccag agtgatagcc ggtgaatata catgcatcaa agctttcatc aattcctgcc
    13981 atcataacta gtgggtcttt ggtccatcct ctaagtattt tgacgttttc aggtaaaata
    14041 tttggattaa tatttctacc actgtcatgt gcatctttaa caagtatttc atgtacacca
    14101 gattgagtag ctccatcaca tgctgctgtt atctctttac tcatttgcgt tctatagtgt
    14161 tgcgaaaatt cacattcaag ttttgtttca tcccaattta caataccaca agtaccttct
    14221 atatcagcac ttaaatacaa tttcataata tataccccct tatgtttatt attgatgata
    14281 ataacagata acagaaaata cataatcaat aaagggcata aaaatattat gtcttactat
    14341 taactattat taaatctgta actgaaaaat aactaaattg gtataaaaaa atataacact
    14401 atgtgtcatt tatttatata gttttttata tttgatgttt atataaaatt gtaacatata
    14461 agtggttaca tttactagtt taacatagtt aaaatagtta ggtattattt ttaagttaaa
    14521 ttgattatta taaaatcgca gaataaaaga aaaatttaat ttattaaaat gcatgaaatg
    14581 ttttttcctc aacataatgc taaattaaac tcaaaaagaa ataatagaaa aatcttatgt
    14641 agcttagaaa gaatgtgaat gatttaaggg aaaaatagaa atttattata taatattagg
    14701 ttgtattata taatacagcc taatattata tgtatatgtg acataaaatc ttggtaagtg
    14761 taaataaaat aaatgtggaa aacaagttaa tgaaatcaag aaaaaagtaa tttaaaattc
    14821 aatttcatct tcccaattaa attctcctga tttttctaca ctcttaggcc agtgactaca
    14881 ccatttagga acggacggat tttcaaccct atcaacgctt ggagtatatg gctttaagtc
    14941 aataacagga ctattatcat tagcatcaat atatggtatt tttattatgc cattttcatg
    15001 gtcgatattg ataatttgta cagcagtcaa tgctattgga ttgggtctta taggtgacct
    15061 tgttgcaaat attcctataa cgtctggtcc acttttataa ggttttgggg tttctaaaat
    15121 acttcttagt tcaggattat caaacccatc agcccaccaa aatacattta aatgactgaa
    15181 atcgtcgagt tcatttaatg caggtacata ctttttgtct aatttaataa agacctcttc
    15241 gccttgaata catattttac ctataggatt tattaatatt tctttcataa tattacctcc
    15301 aactatttaa tttatcattt gtaattttag ggtataacat gacatcatgt gagggtcaat
    15361 ataaaataat aaataaaaat attaatagtt agaatgataa tacatttttt acatggagca
    15421 tgacaccagt aataaggcaa tcttcgtcaa tatcaaaagt agaggtatgc aaaggggaag
    15481 taatgttttt ttcacgattt gcacaaccaa ggtggtaaaa agctccttca cagtgtctta
    15541 gaaaatacga aaagtcttca gcaccaagag tgggcgagtc tttaaggata atattttctt
    15601 caccaaacaa ttctacagca ttttgtttga cgtaatctac tagatggttt gaattgataa
    15661 gagctggata accttcctca atttcaacag aaatacttcc tccaaatgtt ttacaagtaa
    15721 aatcgcaaat ttctctaatt cgtttttttg aaaactctct agtttcagga gtcaaggttc
    15781 tcaaagtacc ccttataacc actttgtcac aaactatatt ttctttgatt ccaccttcaa
    15841 tctttcctag agaaataacc acagaatcag ttggatttgt gtttcgacta acgattgtct
    15901 gtagagctgt aataatatga gcagatataa caatagcatc aataccttga tgtggataag
    15961 ctccatgaca ttttgaacca tgtacagtta attgaagtgt attggtggaa gcatttagag
    16021 tgttgtattt taattcaatt agatttttgt tgatattggg atttacatgg agtccaaata
    16081 tatattttac atttggtttt tcaagacatc catcttctat cataagttga gccccaccaa
    16141 tagtttcctc agcaggttga aaaaagaatt ttacatttac atttaagtaa tcctttaatt
    16201 taaatagtat attacaagta cccagtaaaa tagcagtgtg tgcatcatga ccacatgcat
    16261 gcattttgcc aatattaata gatttataag aataattaag ttcttctgtt ataggtaagg
    16321 catctatatc agctcttata gcaactgttt caaagtttgg actcacgtta atgtatgcag
    16381 ttatccctgt atgattctta tattcaatat aatttatacc tatttcattt aagtatttaa
    16441 taattttttt cttagtttga aattcttcta atccaagttc tggagtctta tgtaaatctc
    16501 ttcttatatt tataacccaa tcatttatct catgacaaag agtttttata ttaagcataa
    16561 aacacatcct aactatatta aaaatttatt tttcttcatt aattatatta ttttatgtaa
    16621 atcattatta tgtatcaagt acaaataaaa tatcttataa ttatattaga gatatttagt
    16681 atggagaaga atatatcatt ttataagaag aggtgtttaa atgttaggaa taattgcgcc
    16741 atctgggcca ttaagaaata caagtttgga ggaaataaag tttaatttag aaagttatgg
    16801 ttacgaagtt aagttttctg aaagttgtag tttaaattat aaaggttatt tagctggtaa
    16861 tgatgatatt agagcaaggg atatagaaga tatgttttta gataaagatg tagatataat
    16921 tatgtgctta agaggaggtt atggaacaac aagaatattg gataaaataa attatgatat
    16981 aataaagcaa aatccaaaac catttatagg tttttctgat ataacaggtt taaatttagc
    17041 tttctataaa aattgtggtc ttttaccata tcatggaata atggcagcaa atgtaggtaa
    17101 atggggtaaa tttacctata aatcattggt aaatgcttta gaatttaaag atgaattata
    17161 tttagaaaat ccaaaagaag aaaagattta tactgtatgt gaaggtaaag cagagggaat
    17221 aataatggga ggaaatttat ccttaataat tgccacaatg ggaacaaaat atgaaataga
    17281 tgcaaaagat aaaattttgt ttattgagga aattggagaa cctcaatata aactggatag
    17341 aatgttaaca caactatatt cttcaggaaa acttgaggaa tgtaatggta taatttttgg
    17401 agattttaag gattgtattg aagaaaatga tttgatggaa ttactaatag aatttgcaaa
    17461 taaggtcaat aaaccttcta tatataatct tcagtcagga cattgtatcc caatgataac
    17521 tattcctttg ggcagaatgt gcgaattaga tgctacagaa aaaatcgtta aattaaaaaa
    17581 atagtgtgta tataaatata atagaaatat ggtataatct tatacaagta aaaatttagc
    17641 taggaggatg aaaaatgtct gatgatagaa taatagactt taacgaacta aagaacaaag
    17701 ttaaagattc tgatgtagat aaatttgagc aatacatata taacttatat ttttctgtta
    17761 tggatggaaa gatgtctatg gctgagtttt caagaaaaat attcgattat atgagagata
    17821 acaacatatc tcaagaaaaa tttatgaaaa tacaaaagca atttatggag agatatggta
    17881 tggacactga agaagtagaa aaacaactta gaaactttgg aatcgaccct tctacagcag
    17941 gttttatgag taataatact tccagcaaag tatctacaga agatttagag tcatttaaga
    18001 agagtgcagg attttatgag aaatatggag aaaaaatcca accaaagagt tgtataacaa
    18061 catttataaa aaatgactta aatgatatta atgtaataat cgaccaagaa aagataatgc
    18121 tttgtagtga tagaaaaata aatcttatgg attcagaact aaatgagttt ttattagaat
    18181 ataagaatat gtttaataaa aaaataaagg tagtaatgtg cgaaactacg aataagtatg
    18241 attattaaaa aatgaattta aatactgaag tagtataaat taattgtact acttaaaaat
    18301 aaaaatcgca atttattatt ccttataaat ttgtataagg aatatctaaa ctgcgatttt
    18361 ttatattgtg aagattagta ataatagata gtgttttaaa ttgtaatctt ctcactaaaa
    18421 ctgtgttttt ctattagaaa ttttattaaa aataaatatc tgataattgt attatatggg
    18481 atgtaaagta ttagaagctt aacgaatctc ttttaataca ttaactccat tttgaatcat
    18541 actgttgaaa aatactaagt ttaaaatctc agcagggtga acgttaggaa tatcataagt
    18601 atctactaaa ttcataggta caactatatt tttttcaata ttatgttgat taaaatatga
    18661 ttttagagtt attgcaaatt gatatataca tatatctgtg caatcgccaa ctataattat
    18721 attatctata ttatcaaaat ccaaattttc taatgcaaaa aatccattag tagaattttt
    18781 aggaagaatt tgtaggtttt ctatcgactt aagttcatca accaattcac tttctacatc
    18841 attttccaaa cagtgaacag gataactcaa aagttctatt gatttaggag tatgagaatc
    18901 agtaaatgct attactctat ttaatttgtt tgatattttt tttgtaaaca tttcaattgg
    18961 ctttattaaa gactctactc taggagagta taatgcacct tgtcttgcaa agccattatt
    19021 aacatcaatt ataaataaag cagttttact caaatcataa ttttctattt tttctattgg
    19081 tagattatct aaattactct ttaaagcttc aagttcattt aataagttat ccatagttat
    19141 acctccaaga taaatttaat aaaattgtac atattaatta tataaaagtc aaggagtaca
    19201 aactattgta taattagctg tattgttttg agtgtaaaag atttgaagca atatctggat
    19261 agttagtaaa aataccatct acattgagtt ttagacagtg tagtatatca ccctcttcat
    19321 ttacagtata tacattgaca ccaagtagat ttttgtgaca aagttcaatt agagattcat
    19381 ttactaaaac tagaggtgga tgaagataac tattacccag aaatttagca aaattgacta
    19441 cattgtttat aggtaaataa cataataatc ctgtttttat actagggtct aatttatgaa
    19501 agttttttaa actattgctg ttgaaactag atataagaac tctatttttc atattatact
    19561 ttaaaataag ctctaaaacg tctttttcta tattgggata gtttattttg tcagttttta
    19621 gttctatatt taaaataagg tctgtaggtg aaattagaat taatacttct tctaaagttg
    19681 gaattttaca ttctttattg ctttgatatc cttcaaaaga tgagtttaga gtttttaatt
    19741 ttcttaaagt aaagtctttt acaaaaccat gtccattaaa agttctatca accttttcat
    19801 catgaataac tacaattttt ccatccttag ttctgtggac atcaagttct attccatata
    19861 tatccatatc caaacatttt ttaaaagcta gaatagtatt ttctggatac tttccactaa
    19921 accccctatg agcataaata ttcatttata tggttccctc ctataagtat aacttatctt
    19981 ataaaaat
//
LOCUS       DAEMGG010000067        19969 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEMGG010000067 DAEMGG010000000
VERSION     DAEMGG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.067"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(261..404)
                     /gene="scfA"
                     /locus_tag="KPK24_002265"
     CDS             complement(261..404)
                     /gene="scfA"
                     /locus_tag="KPK24_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG2299935.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(470..832)
                     /locus_tag="KPK24_002266"
     CDS             complement(470..832)
                     /locus_tag="KPK24_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG2299936.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(890..1183)
                     /gene="yajC"
                     /locus_tag="KPK24_002267"
     CDS             complement(890..1183)
                     /gene="yajC"
                     /locus_tag="KPK24_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG2299937.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1317..2438)
                     /gene="tgt"
                     /locus_tag="KPK24_002268"
     CDS             complement(1317..2438)
                     /gene="tgt"
                     /locus_tag="KPK24_002268"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG2299938.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2517..3002)
                     /locus_tag="KPK24_002269"
     CDS             complement(2517..3002)
                     /locus_tag="KPK24_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299939.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(3014..4039)
                     /gene="queA"
                     /locus_tag="KPK24_002270"
     CDS             complement(3014..4039)
                     /gene="queA"
                     /locus_tag="KPK24_002270"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG2299940.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEYGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4357..5376)
                     /gene="ruvB"
                     /locus_tag="KPK24_002271"
     CDS             complement(4357..5376)
                     /gene="ruvB"
                     /locus_tag="KPK24_002271"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG2299941.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5483..6085)
                     /gene="ruvA"
                     /locus_tag="KPK24_002272"
     CDS             complement(5483..6085)
                     /gene="ruvA"
                     /locus_tag="KPK24_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG2299942.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(6078..6587)
                     /gene="ruvC"
                     /locus_tag="KPK24_002273"
     CDS             complement(6078..6587)
                     /gene="ruvC"
                     /locus_tag="KPK24_002273"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG2299943.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6930..7559)
                     /locus_tag="KPK24_002274"
     CDS             complement(6930..7559)
                     /locus_tag="KPK24_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299944.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7762..8085
                     /locus_tag="KPK24_002275"
     CDS             7762..8085
                     /locus_tag="KPK24_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG2299945.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8353..9207
                     /locus_tag="KPK24_002276"
     CDS             8353..9207
                     /locus_tag="KPK24_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299946.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHTIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9393..10829)
                     /locus_tag="KPK24_002277"
     CDS             complement(9393..10829)
                     /locus_tag="KPK24_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG2299947.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSEKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKILGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10900..12282)
                     /locus_tag="KPK24_002278"
     CDS             complement(10900..12282)
                     /locus_tag="KPK24_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2299948.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKINPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12418..13323)
                     /gene="garR"
                     /locus_tag="KPK24_002279"
     CDS             complement(12418..13323)
                     /gene="garR"
                     /locus_tag="KPK24_002279"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG2299949.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            13719..14390
                     /locus_tag="KPK24_002280"
     CDS             13719..14390
                     /locus_tag="KPK24_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2299950.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            14493..15041
                     /locus_tag="KPK24_002281"
     CDS             14493..15041
                     /locus_tag="KPK24_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299951.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            15303..15611
                     /locus_tag="KPK24_002282"
     CDS             15303..15611
                     /locus_tag="KPK24_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299952.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(15773..17563)
                     /locus_tag="KPK24_002283"
     CDS             complement(15773..17563)
                     /locus_tag="KPK24_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299953.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(17563..19290)
                     /locus_tag="KPK24_002284"
     CDS             complement(17563..19290)
                     /locus_tag="KPK24_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2299954.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(19535..19801)
                     /locus_tag="KPK24_002285"
     CDS             complement(19535..19801)
                     /locus_tag="KPK24_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBG2299955.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
BASE COUNT         6627 a         3438 c         2290 g         7614 t
ORIGIN      
        1 cataaattag agtttaaaat taaaaattat tctaatacat gtaattgata aaaaataaaa
       61 tataagagta gctactttaa aataaatgct cttacatgat tagatatttt tgcataatta
      121 aacgataaga aatcaaattt acttctacat aaatagaata tatattttta tataataatt
      181 gaaaataaaa gaggatacct cataatgata aaagaatcac tttgagatac cctcttacaa
      241 acaactaaat caataatttt ctatctttca cactcttgat ttgcaactgt acaagaagtt
      301 ttacaagctg attgacaaga agtttgacac tctccacatc caccttttgc agcactttgc
      361 tttaaagtag cttgagataa agtttttaca tgtttatttt ccattttcaa cgcctccatt
      421 tctaattgtt tcctattgat tatatcataa aactacttta atataaactc tatttaaagt
      481 tgacacccaa tacgcctcca atgcctcctg ctgctgatat tagcaataac ttataaatca
      541 tccctatttc aaatacaaac ttttcttgtg ctaaaaacac agtcaaaaaa atacatacta
      601 tgtacaaaag tcctactaaa agcccataca taagtccttt ttctttcatg tgttttgaag
      661 tgtaaaaccc tcctatagct gcagaaatag tagttataaa tgatgagacc atagttatat
      721 tatctccacc aatatctgta aatgtcaaaa ataagttata cactaataaa acagccaaag
      781 ttattatata tgcatatcct agacctttaa aaatataatt agattttccc ataaaaaaac
      841 ctcctctcaa attaatatat attcgagaaa aggcttttaa attactaatt tagtcttctt
      901 ctatttcttc ttttttagat ttaacagttc ctatagccca tttttcaaat ggaactttag
      961 ttctatttgg tcctaattct aatattacaa catcatcttc aactttagct acattagcta
     1021 ctattccacc tattgtaatg accttgtctc ctacacttag gctgcttctc atttctttta
     1081 attgtttgtc ttttttcttt tgaggtctaa tcattaggaa ataaaagatt gcgattacta
     1141 ctacccatag acctatactc ataattattt gttgctgtgg cattaatttt tcactccttt
     1201 gtaaattata ctcttttgta aatcataata aatgtttata taacaattta ctttctctat
     1261 ctttgagtac tattgaatat tatatattaa aatgattata attaatagtg caaaacctat
     1321 atttcatatc catattttgc aaaaaattca tttctaaaat caagtaatct atcttccatt
     1381 atcgcttgtc ttatttgttt cattaaattt aataaaaagt gaagattatg agtagttatt
     1441 aatcttgcac ccagtatttc atttgctttt ataaggtgtc ttatataagc tcttgaatag
     1501 tttttacaag cataacaatc acattcaggg tcaagtggag taaagtcttc agcataagtt
     1561 gcatttctaa ctactacttt accttggcta gtcattgcag ttccatttct agcgatacga
     1621 gtaggtagaa cacaatcaaa catgtctact cctcttataa caccttctac taagtcatct
     1681 ggactaccaa ctcccattag gtatcttggt ttatcctttg gcataagtgg agttgtatgc
     1741 tctaacacgt catacataag tggttttggt tctccaacac taagtccacc aatagcatat
     1801 cctggtaaat caatattagt tatttctttc gctgattgtt cccttaagtc tttatacatc
     1861 ccaccttgta ttataccaaa taaagcttgc ttgtcggtat tattatgggc atctttacat
     1921 ctttttaacc atcttgttgt tctttctaaa gaatttttta catattctct atctgatggg
     1981 tatggtgcac actcatcaaa tgccatcatt atgtctgaac ccaatgcatt ttgtatttcc
     2041 atagcttttt ctggagttag aaaatgtttt gaaccatcta agtgtgacct aaattctaca
     2101 ccttcttctt ttatctttct taaaggccct agactaaata cctggaaccc tccactatct
     2161 gtaagtattg gtttatccca attcataaac ttatgaagac caccagctct ttttataagt
     2221 tcatgacctg gtctcatata taaatggtat gtgttactaa gtattatttg tgaaccaatc
     2281 tcttttaatt cttctggtgt catagatttt acagttgctt gagttccaac tggcatgaat
     2341 attggtgttt caataatccc atgtggagtg tgtaatcttc ctaaccttgc accactctgc
     2401 ttacaagtct ttatcaattc gtatcttact gcgtacataa agttctcctt aatcatattt
     2461 atttctcatt atttcttaag atatatttgt aataagctat tcatataatt tattgtctaa
     2521 atagatttat cttcgtcttc tttgttctgt tgttccttaa ttgcttcttc atcatgaata
     2581 ctcattatat taagagcact cttgtctttt gttactaaaa acttttttat agcaaatagt
     2641 cctgctatag ctataactcc tattaaaaca tctatcatac cttcattttt aggcagtagc
     2701 aacatttttc tagcaattgc gtaaagtaat gcctcaataa atgctcctgg tatatgaaga
     2761 gatagcatta ctacaagttc aacacctatt actaaaagta atatttgtgc taaaaaatca
     2821 ttaagctgac tgtattgtac tggattttga aaatcaacta tatatgctcc ccacataagt
     2881 cttagaacat ctatagttcc taaaaatact gctattagaa ctactactgc taaaacacgt
     2941 tcaaatataa aagcaatctt taatataggg ttattctttt tttgtttttg taaactatgc
     3001 atccattacc tccctacttt ataatcatag catctccaaa actgaagaat ctatatcttt
     3061 cttttactgc ctcattatat gcatttaaaa cattatcctt tccacaaata gcacttacta
     3121 acattattag tgtagactct ggtaagtgaa aattagtaat aagtttatct aaaactttaa
     3181 atttataacc tggatatata aatatttctg tccatccact agtttctttc atctttccat
     3241 actcattaca agctgattct atagttctac aactggtagt accaacacat attacatttt
     3301 ttccattttc ctttgctcta tttattttgt cagctgcttc ttggctaacc atataatatt
     3361 ctgaatgcat cttgtgattt aaaacatcct ctacttttac tggtctaaaa gttcctaagc
     3421 caacatgtag agttacaaag gctatatcta cacctttttc ttttatttta tttaacaatt
     3481 cttctgtaaa atgtaatcct gctgtaggtg ctgctgctga accattatgc tttgaataaa
     3541 cagtctggta tctttctttt tcatctagtc tagcagtgat atatggaggt agtggcatat
     3601 ttccaagttc atccaatatt tcttcaaaaa ttccatcata atggaatttt ataattcttg
     3661 aaccttcatc tgataaatct acaacttctc caattagctt tccatttcca aaagaaaatt
     3721 tagtccctac ttttgctctt tttcctggtt ttactaaagc ctgccaagta tcttcctctg
     3781 ttctttttaa taataaaaac tctattttac ctccagtttc aagcttctct cctattaatc
     3841 tagctggtat aactctagta ttatttaata ctaagcaatc tcctggattt aaatattcta
     3901 ttatatctct aaatacttta tgttcaatat tacctgtctc tttatctaat accatcaatc
     3961 ttgagctagc tctatcttct ataggaactt gagctataag ttcctgtggt aagtcaaatt
     4021 taaaatcact tgttttcaat gttaaacttc ctctcttaaa atttctccag ctacaataat
     4081 atcaaaaaaa caacttattt tccacaatat taccataatc tatcaagaaa ttgctatttt
     4141 taacagacac catatccaca atatatagat atttttccac gatatacatg tcttatccac
     4201 aatatataga tacaatacac acaattttta tcgtgtttat ccacagtatc tacagatata
     4261 attaccattt tgtggatatg tttgaatttt tagccatttt agaaatattt taatttaatg
     4321 actcttttat tttataaata ctcgtataat ctatttttat ttttcattag gatatggaat
     4381 ttttaagtgt tcatatgcta atggcatagc tactcttcct ctaggacctc tatttataaa
     4441 tcctaattga agcaaatatg gctcatatac atcttctatt gtatttctat cttctccgat
     4501 agaagctgcc aatgtatcta aacccactgg tcctcctcta aatttttcta ttatagtcat
     4561 aagaagcttc tcatctacaa aatcgagacc caaactatcg actcctaaaa gttccagagc
     4621 atcttttgcc accttatcag ttatttttcc atcagctctg acttgtgcaa aatctctaac
     4681 tctttttagt aatctatttg ctatccttgg agtccctctt gaacgttttg caagttctaa
     4741 tgctccatta gactgtatct ctgcatctaa gatgcttgaa gacctaagta ctatttttga
     4801 aagttcatca actgtataat agtccaattt acaaataaca ccaaatctat ctctaagtgg
     4861 atttgtaagc ataccagctc ttgtagtagc tccaataagt gtaaatttag gaaggtcaag
     4921 tcttatgctt ctagcagatg gccctttccc tattattata tctaggcaaa aatcctccat
     4981 tgctggatat aaaacttctt caacacttct attgattcta tgaatctcat ctataaataa
     5041 aacatcattt tcatttaaat tcgtaagtat ggctgccaaa tctccagctc tttctattgc
     5101 aggtcctgac gtaattctta aatttacccc catctcattt gctattatac ttgctagtgt
     5161 agtttttcca agacctggtg gtccatataa aagcacatgg tctaactgct catttctaga
     5221 tttagctgct tctataaata tactaagttg ttctttggat ttttcttgac ccaaataatc
     5281 ttctaaagtt tttggtctta aactattttc tatatctata tcttccatct tcattgtaga
     5341 agttattatc ctattttcat cttcaaaacc ttgcatttat acccctcctc tcatactttt
     5401 gaaaatctac tttatatatt ttcaaattta cataaactcc ttaaatattg ttttaaaatc
     5461 caaatattat aaaatccaaa tactatttca aggatttact cattatatat gtaagtgctt
     5521 tctttataat agcttctgta ttcataccat cttttttaca tttttgaact gcctcttttg
     5581 attccgacaa agtatagcct aaagctacaa gtgcatctac agactcatct tgtgatatca
     5641 aggtaggttt ttgtgataat aatgttggtt caaactctat gttatttttg tctaccttat
     5701 ccttaagctc taagacgatt ctttctgctg tctttttacc aacaccagga gctttggata
     5761 attttccaat gtcttcactt aaaatatatg ctcctaactg tgctggtgat gcaaatgata
     5821 gtattgaaag cgccacttta ggtcctattt tagatacaga tgttagtaat tcaaacatct
     5881 taagttcttc cctacttaca aaaccacaaa gactcatatc atcttctctt acaattaatt
     5941 tagtatatat ttttgtggct tctcccactt gtaaattcat tattgtattg gatgatacat
     6001 ttattttata tcctatccca ttattttcaa caactatact gtctatataa atttcttcta
     6061 ctgtaccttt tatataacta tacatactta ccacctatat tctttagagt tttttcaagt
     6121 ttgtttgaat gagcatggca aatagccaca gctaaagcat ctgcaacatc atctggtttt
     6181 ggaacttttt ttagacttaa aaatgaagtt accatctgtt gaacttgtgc cttatctgct
     6241 ctcccatatc caactacacc ttgttttact tgaagtggag tgtattcata tacaggcttc
     6301 ccattatgag cacatgcaag catagtaaca cctctagctt gtgcaactgt tatagctgtt
     6361 tttacattct tgttaaagaa taattcttcc attccaactt catctatatt gtaattcttt
     6421 actattgtat caattccttt atacacaagt tccaatcttc tcgatatatt catatgggca
     6481 ggtgttgtaa ctgctccata atcgattact ttaaacttgc tatttttgta ttcaattata
     6541 ccatatccaa ctatggctat acctgggtca atccccaata ttatcatttt ttaaaattcc
     6601 ccctaaaaat aaaagaaata ccaaaatgta tttctcataa aaacttatgt tctaaatcat
     6661 tatatcataa ataatattaa caaatcataa aatgctctat gtaaaatata aaatcaaata
     6721 aaaaagttat atcaaagcaa gattttacct caatataact tttatattta ttacaaataa
     6781 attattgtat ttttatagga attaaatgtg actacaattt taatactaat ctatttaata
     6841 ctaatctatt taatattaaa ttatttatta ttaatttact tagtatttaa cttacttagt
     6901 atttaactta aaataatatt aatttactat taacttgcaa agtcttctaa tcttgaatag
     6961 gcctcttctt cagtatccat aactacacct tttttgattt catcttgaac tgttttagga
     7021 agtgaatcta ctcttatttt tgttttttct actaatgttt tgcttccact atcatcatat
     7081 ttatatagtg ctatatttcc attatcattt tcaatagtat atttaccttt tatagtaaca
     7141 tccattgctt gtacatcttt attatcacta tatttagatt ctaccaatat gatttcattt
     7201 ttatccatac tctttatttt tttagttgga tattcttttt taaaatatga tactatttct
     7261 gacttcgtct tatctaataa tgactctgga actattcctg ccattgtaag tgttctctta
     7321 tagttagttt cattttcgcc ttcactttga agccaaactt cacaattttt atttaatcca
     7381 aaagcttctt ttgacttttc attcatattc gcactagtac ttacaacttc accagtttga
     7441 actccattgt gttttaatct tgtccctata taaactccgc tacttaatac aaatactcca
     7501 accagtatta aggcagatat tttccaagga aataattctt ttttcttctt ttcaaacatt
     7561 gattacgcct cctttttcta ttattttctc caagctgtaa gtttttatac aatttaggta
     7621 aattttagaa gaaaatatat ttatatttca aattactcta agaatagata ttttctaaaa
     7681 ggtagttttc tcttaatggt tatattcttg taattatatg gacataattt ataataaata
     7741 atcgtttagg aggtaataac tttggaaaga ataaactgta atgtaattaa ctgttcacat
     7801 aacaacagcc atatatgtta tgcagataaa atttttataa atggtgaaaa agctcacaat
     7861 gacagacata cttgttgtag ttcattttta gatgaatcta tttatagtaa tttatctagc
     7921 aacgttaata atagaaattg tgaggaatta gtttgtactg taaaaaattg taaatacaac
     7981 aaagatggta gcttatgtac acttgataat atattggttg ctcctgaaac tgatagagta
     8041 aacctttata ttgaaacatg ttgctctagt ttcgaatgta aatagtcata gtataaatat
     8101 aagggtgtca aatgtaatta aactaacata gtgtagagta tctacaacta ttagtttgat
     8161 tatacttgac acccttattt attttaaata aaatttcatt attataacca tgtttataat
     8221 gttctaactg taattaaatt tgataaattt ctataaattg gtataatcaa tatgtaaatt
     8281 gaatttagta aattaatcat aaaaataaaa tatcgagtat atagtgttat actaactaga
     8341 aatgaggtgc ttatgagtac attttatatt caatttcttt attttgtaat ttatagcttt
     8401 ataggatggt gttgtgaaac tacatattgt tcagttttac aaaaacaatt tgtaaataga
     8461 ggttttttaa atggtccatt ttgccctata tatggatttg gagcattagc aatagttgct
     8521 accttgactc cttttgtaca cactattcct ttgttgtttt tgtttagtat aattataact
     8581 agcataatgg agtattgtac gagctttatt ttagaaaaaa tatttaatat gacttggtgg
     8641 gattattcta aacataaatt taatattcac ggaagagttt gtctggaaaa ctcacttatg
     8701 tttggtgtat tatcattgat tgtaatgctt atagtacatc ctattgtggt tgactttatt
     8761 aactctatct ctaaaaatgt gctatttata tttgctatat ctatagaaat ttattttgtt
     8821 ttagatttag ttatcacagt ccatacaatt ttacaattaa atggtaagtt aaaacaaatc
     8881 aatcttataa ttaaggaatt aaaagataag aaagaatact acaaacttat tacacaagaa
     8941 actatagaaa acaaacttga atctttagtt gaaaatagac tggatacttt ggaagatagg
     9001 tttgaaatac ttaaagaaaa agaaaattat cgctatgcta aaaatcgtat tgatgagcta
     9061 aaaaataaac ttaatacttt gctgtctaac cacaaattac ttcatagacg tataatcaaa
     9121 gcattcccca acataagctc taataagcat acagatattt taaataaaat caaagaaaat
     9181 attaaatcaa ttaaaaaaga taaataataa aataaacaca taagttaaat aataaaaata
     9241 ctaatacata agattaaggt ttagaatatt ttttgtaaaa tactctaaac cctaattaat
     9301 atactattat ttttaatctt aatttataat tttagtttat tttaatttta actcagtaaa
     9361 tttaagcgtt taaacttcaa tacactaata tactacccca tcaatatctc aactgattct
     9421 ctaagtgtgc taatctctcc cacatttcca gggaaaatta tatatggcat attaggaaat
     9481 ttgctttctt tgcctgtcat ccatactggt ataccaggtt ttatctgtcc cattactgta
     9541 gcttttttta ctcttagtgc tttagtacca acatcactag aagtaattcc acccttagct
     9601 ataataaaat ttggtcttac attaagcata ccaattatac tggtaactgc atctgatatt
     9661 tctacagaaa tcataagctg tttatcttta tcattcgtat ccaagtcaaa acgctctcgt
     9721 ctagtataaa cagcaacagt tttacccctg cttatttttt cctctacaac ttttataact
     9781 ctttttacct cattttttag accattttct tgaagaacta aatgttgatt aaattctata
     9841 aattctatcg gatatttaca atttttaagt tcttctaact gttgggtagt cttattcaca
     9901 tgtgaaccaa caagtataat tcctccattg gtattacctt ttgatactaa ttcatcctta
     9961 gtaagtagtt ccttgtcact cactccgcca agtatttttg ttatggcagc agcgcttcta
    10021 aacataaatt ccttaccact atttactgct cgtataaacg caattgtaaa tacctttaca
    10081 tctacataat cggttgcatt tacaataatt ttattgaatc cttcagcttt tttaagtttt
    10141 tctgttatag cttcaatatt tagctctcta agttcttttg acgaaatata tatcatatca
    10201 ctagatttgt actttccatt tgtcttttct tcacaccact cacctatatc agatgattta
    10261 tatccaaaag atttatcttt tgcaaattca gtcattcctg ctggtacaag tatttctcct
    10321 tctttaacat aatgtacatt atctatagta aacctaccac cttctttaaa aaatggcatt
    10381 attatttcac cctgaaataa cttctccgat aatctctcta cttcgctttt taataaatta
    10441 gtttcaagag gataatgtcc tctcatagta gaatcactac gacttatgat tataaaatcc
    10501 ttattaactt tctttgatac atccacaata ttctttgaaa tttctttatg tgcttttgta
    10561 gtttgagcaa cagtaaatcc ccttgaattg gtaagtataa aaaacatact atttttctca
    10621 ttaaaacctt gttccatact atccttatcc caatcagtat aaacagaaat atcatgaact
    10681 gtttgtacac ctgttgggtc atcatctaat acaacaattt ttctatctaa atgcttaagc
    10741 tcatctgcaa gaagtttatt taatctgtcc tcatctactt gtttaaaagt attaagtata
    10801 tctatgctaa ttggttttac ttctctcatt taaaacgccc ctttatgtaa tttgtatagt
    10861 ctaaatgtaa tttatatgat ataaacaata gtttgttatt tatagacctg gaagtggtat
    10921 aattccattt ataacactaa gcaataaaac cattataaat gctacaactc cagcaatggt
    10981 tcctccaatt gtccaagagt ttaaagtatc tttaactgat aatccactaa atcttgaaag
    11041 tacccagaat ccagaatcat ttggaaggga gaaaccaact cctccagcac atatagcaag
    11101 ccctactaaa actggtgaca ttccagttgc caaaattgta ggtcctaaaa ttgatgatgt
    11161 tgttactaag gctacagtag cagaaccttg agaaattcta agcaaggcac ttaataagaa
    11221 tccaagcact acaactgata tactaagacc agacatagta tcaactaaga agttacctat
    11281 accacttgca tttataacac tgccaaacgc accacctgac cctgttatca ataatattag
    11341 ccctgctgaa tcagcagctt caataactac atctcccata ggtttattta tgtatttctt
    11401 taaaaatcct attgccacca atattccaag aagtattgct atgtttttat ctcctataaa
    11461 tgcaagtact ccactcattg tagaaccttt ttctaagaac atactaaata catttgatat
    11521 aagtataaga agtattggga acaataaaga aaacatagaa attccaaaac ctggtacttt
    11581 agaagaatca tcatcttcaa tttttatgct atcttttccc tgctctaaat ttttttctct
    11641 atcattctca tcaatagcat aagctggata tttttcaaaa cgttttccat aaatccatcc
    11701 acctataaga gctgctggaa aagcaacaat tatagaataa agtataaaac tacctacatt
    11761 tgcatttaca tttgctgcaa ctgctaaagg tcctggtgta ggtattacaa gtgcatgacc
    11821 tactatagtc ccaactccta aggcacatac aaaaaccatt agagatttct tagttactct
    11881 cgaaacatac ttaattatag gcatcattat aacaaaagct gcatccataa aaactggaat
    11941 tgaaattaaa aaacctgctg tagttactgc taaagcactg tttttttctc cagtcttagc
    12001 taacaacccc tttgcaatag attctgttgc tcttgattct gaaagaatat ttccaaatat
    12061 tattccaagt ccaattacaa tacctatact acctaatgta tttccaaatc cagtcgatat
    12121 tgtggttgat atttcattta taggcattct aaccataaag cctgtaatta tagatgttat
    12181 cagtaatgcc aaaaacggat ttattttaaa tttaataatc gatacaagta aaattcctat
    12241 agatataaca aatgctatca aaagtccata tcctgtaatc ataatcttta cccctttatc
    12301 attctacttt attatttatc aattttattt ttcttttact atgataactt aacctcgctg
    12361 tatttatata atctaatcct aataatatgt atttaattat attgatatat ttaaatatta
    12421 attattgtgt ttttttattt caacacctgc caacttttca aaatactgtg ctattccact
    12481 atggtcatct tccaaatgtc catgtacttt taatgtctgc ataatttcta ataattgact
    12541 tgtcataggt agtggtacat ctaaattgtg tgctgtttgc attacattct taatatcttt
    12601 caaattaata gatattttcc cacctggttt aaaatcacga ttcataatca ttggtatctt
    12661 cgcatccaat attgtactac cagctaggcc tcctcttatt gccttatata ctttttctgg
    12721 gtctgcacct gcttttgctg caagtacaaa tgcctcagaa actgctgcta tcgtcaaatt
    12781 aacaattact tggtttgtaa gttttgtaac acttccacta ccattatctc caattaaaat
    12841 agatgagctt cccattatat taaaaaatgg ttctactttc ttataaattt cttccttacc
    12901 accagccata aaagcaagtg taccatcaat cgcttttggc tcccctccac ttactggtga
    12961 gtctataaat tctaagccca tatcttttaa tttatttgca cacaatatgg attctgtagg
    13021 agtaactgaa ctcatatcga ctactatact tccttctttt aaagtttttg ccaatccatc
    13081 catcccaaac aatatatcct gcacaatagt tccatttgga aggattgtaa atacaatatc
    13141 acattcttga cccatttcta aaacacttgc gtattttgct ccacatgata taagttcatc
    13201 cacagcagat ttattaatgt catacaccaa aagattacat ccatctttta ataaattttt
    13261 tgccattggc tttcccatta ttcctaatcc aataaatccc aatttcattt tataaccctc
    13321 catatcttca catttttgaa aacgtttttt atgttttaga gtatactaca taccagtata
    13381 ccggtcaata taaaatataa aagtatttat tttaacaata aatagtaatt tttaaagtca
    13441 tttattatat ttagtcatcc tatataagtt ttaatacata ctattgtttt caaatataat
    13501 atactacttt tacagttttg tattttaact aatttcaatt agttttaatt aatctgcctt
    13561 taatgttaat catgcttcac acttcaataa ttttttatat tttttaatct tatctgcaat
    13621 ttaaattgca aaagattgac cttattttca ctatgttgta tgcttaaaat tatataagtt
    13681 tttgtatttt tatattgttt aggggggtat tattaagtat ggctacattt aaaagattac
    13741 aagaacaagc atatgactac ttaaaagaat tgatactatc tggagaaatg atagaaaatg
    13801 aaatatattc tgaaacaaaa cttgctagtg aaataggtat ctctagaaca cctataagag
    13861 atgctcttca aagacttagt caagatggat ttatagatat aatcccaagc aaaggattta
    13921 gaatccatca aattacagca aatgaaattg tagagatatt ccaaatacgc tctgcaatag
    13981 aaggattttg tactttttta attacaagtc aatacaaaga agctagagca gtagaaacta
    14041 taagtaaatt aaaacatctt ttagataaac aaaaagatat tttattaggt gataaaaatt
    14101 taaactcatt tgcagaatac gatacattat ttcacacaac tatagtttca tatgctcaaa
    14161 atactgaatt tgacaaaatg tttaacaatt atatgtacag aataaaaaaa ttagctctcg
    14221 attcattgtc acatgaagga aggcttgaaa caacattaaa agagcacaca gatattttta
    14281 ataatattgc aaatggatgt attgaagata tttacagaac tactctcatt catatggaaa
    14341 ctcctaaata cattaattta gaagattttt gtaacaataa gttattttag cattatactt
    14401 tcatttatta aaaatatatc tatctcaaat taatttccta atttataaaa aatattctat
    14461 aattatattg taactaatac ttattctaag gagtgatttt tttgaacgaa gatgttgtaa
    14521 ctaatgaaac tattgacaaa gattatgcta ttgaagaagt tcgtatggca tgcaaacatt
    14581 ttggtgattt atatttttat ttttccaaag tgttatttga agagtttggt gaagataaaa
    14641 ctagcgaaat attaagaaaa gttttatttg aaaggtctga agaaagagct attgctatga
    14701 gagagagagc tcatgaaaat ggagatgaat taattgcaga caatattatt agcacaactg
    14761 atgttccatt tctaggatgg gttcctgaat ttgaagagtt acattgcccc tatggagctt
    14821 cctggctttt aagatttgag gaaaatcctt ggtttaaaaa attcgcttct ctttactgtg
    14881 atgttacaga tactactgta gctgaagtat ttacaggaga tacgtcccat aaaataacaa
    14941 aaaacattct atggggagat aaaagttgtg aaagaatata tttccatgat gacaaggtat
    15001 ctgaaggtaa atatacttat ggaaataaaa actgtaaata aataatttaa attttctata
    15061 ttcatatact atattttgta ctttttcata taaatattat aactagtctt ttattttact
    15121 tttgtttaac ctaaagataa tttactttta atctatatga attatcatat aaataatcag
    15181 aaagttatct ttataaatta tagttttaga tttataattt ataaaatatt tatgttcttt
    15241 gtaaaatttt ttagaagctt ctaatgttat gctataaaac tatcttgaga agggctgatt
    15301 gaatgtttaa taaaatatat gaaaagaaag aatttatagt ttttcaagtt aaaaaaggtt
    15361 atgtagttta taatactaga aagagttttg aagaaggtca tacacattta aagcattttg
    15421 aagcagcaaa gacagctatt gatttagcaa taaataaaaa gattcctaga agcaaagatg
    15481 gatattatct gactagttta attcgcataa gtgatgatgg ttattacatt gataagttaa
    15541 gcgaattgtt atatgttaga gaacaaaaag gtaaaaaaga aaagtactgt aattctggtt
    15601 atcagatgta acaagactag ttgtgaatta taagtaccaa tcataagtac ctttggtctt
    15661 aaaatcaaag agtatcagta aatctataaa aaaacaactt actgatactc tttttaatta
    15721 tatgattaat atttgaattt gatacaaaat tttatacaaa agtttatgtt atctattctg
    15781 attctttgtc tgcaaattgg ctattataaa gcttctcata aaatccaccc ttttccatta
    15841 attcatcatg attcccctgc tctataatac ttccattatt cataactaaa atcaagtcag
    15901 cattgcgaat tgtagaaagt ctatgtgcta taacaaaact agttctacca ttcataagat
    15961 ttctcattgc tttttgaagc ataagttcca gcctagtatc aacagaactc gtagcttcat
    16021 caagtatcaa tattgaaggg tctgatataa aagctctagc tatggttaaa agctgttttt
    16081 cccctagaga tatattagat gcttcttcat ttaaaaacat attgtatcca tctggaagag
    16141 tggttataaa gtgatgaaca tttgctactt ttgctgcttg tataatttct tctttagtag
    16201 ctccaaatct accatattct atattttcaa aaatggttcc attaaataac catgtatctt
    16261 gaagcaccat accaaacatt gaacgcaaat ctttacgttt catatctctt atatctactc
    16321 catcaatttt aatagcacct ttctttacat cataaaacct catcaataag tttattaatg
    16381 ttgttttacc tgctccagta ggtccaacaa tagctaccat ctgaccactt tttacttctg
    16441 catttaaatc ttctattaaa gttgaatttt ctccatatcc aaaattaaca tgctcaaatg
    16501 taacatttcc ttttacattt tctatcttaa ctggattttc aacatcaggt atttcctctt
    16561 cttcgtctaa aatttcaaat acacgttcta tagctgcaaa agaagattgt attactgtag
    16621 ataactgagt catttgtgaa agtggctgat ttatctgcca tatataacgc acaaatgctt
    16681 gtaaatttcc tactgttatt cctccactta ctgcatatat agaaccgaca actgccacag
    16741 ctgcaattcc aaagtaagtt actaatgaaa ccataggatt catcatactt gatataaatt
    16801 gtgccttaaa tccattttca caaagttctt gatttacttt tttaaattct tttatagaat
    16861 cttcttcttt accatatagt tttatttcat taaatccagt atacatttct tgtactttcc
    16921 catttaattt tccaagagca ttttgttgca atgaaaatct actttgagat ttttttacta
    16981 caaatttaga tacaaaataa ctaattggaa ttataaaaag agctataaga gccatatata
    17041 tattaattgt aaacatcatt gaaagtgcca aaattatgac taagattgag tttacaattt
    17101 gcataaaact ctgctgcaat gcatttgata ttgtatctac atcatttgat atacgactta
    17161 atacatctcc aaaactatta ctatcaaaat aactaatagg caatcttctt attttatttt
    17221 cgacttcatt tcttaaatct ctcattgtgt tttgaatagc attggtaagt agaaaacttg
    17281 aagtatacgt aaaaattgca ttggcaaaat atatacatgc taaaatcacc aatatttttg
    17341 ttacataatc aaaatttacg gaagcccctg gtactccttt tgcgatacta attacatctt
    17401 tagtcaattg tgttattatc aatccttcag ttttaggtgc aagtgcattc atagtagctg
    17461 ctgaaattat acatataata gctataataa aggaaaattt atatttcttt acaaatggaa
    17521 gaagtctacc aattgttttc ttaaaacctt tagacttttt cattattcta attcctcctt
    17581 ttttaactga gatgttgcaa tttcatagta tatttcacag ttttttaata attctttatg
    17641 agttcccatt cctacaactt gtccttcatt gagtactata atcttatcag catcaataat
    17701 agaactaatt ctctgggcaa caatcaatac aattgcatct tttgtttcct tcttcaattt
    17761 agcacgaagt attgcatcag ttttaaagtc aagtgcagaa aaactatcat caaaaatgta
    17821 tatttcaggt cttcttataa tagcccttgc aatagaaagt ctttgttttt gacctcctga
    17881 tacatttgct ccaccttcac taatcaattc atcaaacttt ctaggttttt ttgatataaa
    17941 ctcatatgct tgtgcaactt ttgcggaata ttctaattct gactctcctg ccttatgttt
    18001 cccaaatcta atattatttg ctatgctacc agtaaaaagc agtgattttt gaggtatgaa
    18061 tcctatcttt tttcttaaag cttttaaatc atattctctt atatctactc catttatctt
    18121 aatactacct tctgttacat cataaaatct aggtatcaag ttaattagag tacttttccc
    18181 actaccagta cttcctataa aagcaactgt ttcacctgtt tttgcagtaa atgaaatatc
    18241 cttaagtaca gaagcttctc catctggata ggtaaaagtt acattatcaa attctatgat
    18301 acccttattt tcagtatcct taattccatt ttcaggattt tttatcaatg gttcttcatt
    18361 taaaagttct tttattctat ttgcagatac ctctgctctt ggatacataa taaataccat
    18421 tgaaaaaagc ataattgaaa acatggcatg gaaaagatat tccaaaaatg caaccaactg
    18481 tccaacttga agctctccaa tatttatctt ctcacttgaa atccaaaata cagccaaaac
    18541 agctatattt aatagtaaaa agaatgctgg ttgagtaatt gacataagct taaatagttt
    18601 cttagaaaca ttagcataat atgtatttgt tttttcaaat cgctctgttt cataatcatc
    18661 atttccaaaa gctctaatta ctcttattcc agttaaattt tctctcgata taaggtttaa
    18721 tttatctagt cctttttgtt gttttgaaga tattgcctga gatacttttg ctattattgc
    18781 tactccaata attataaaag gtactgatat agccaatact atagaaagtt ccacacttgt
    18841 tctaattgtc attattatac ttataatgaa cataactgga gttaataagg cagtccttag
    18901 tagtatatta acaaattgtt gaatttgaaa tgcatcattc gttgttcttg taatcattga
    18961 agaaattcca aatttattgt attcagtatg tgaaaattct tgtgatttct caaatatgtc
    19021 atttcttata tctctggtta cacttgttga taccttagca gaacaataac ccaacaatat
    19081 actacctaca acccctataa tagaaataat aacaatcact attcccattt gttttatgta
    19141 agctatattc tgatttgcaa taccattgtc aattatttta gctattatag ttggaatccc
    19201 caactcgact aatgcaaaac taaatatagc cagtatgttt aatactatta ataacttata
    19261 attctttaaa taacttaaaa ttaatttcat atttttctcc tttcttaatt tttgagatag
    19321 tgtaatcatg taataatact aaatttataa atgcttaatt ttataaaaaa atgagaaaag
    19381 agttaacgca ctctcttctc cagcatattc tttttaatac accctccgac aagtcgctta
    19441 tatgttaaca tatttgacaa gtaatgtcaa ttcatatagc caaaagtctt ttaaatgctt
    19501 catttcataa cattgtaaca aattggcatg taatttattt ttgtttttta ctgtattcca
    19561 attttacaag ttctccaata ttactagctt tcttaaaatt caaattagtc ttatctatac
    19621 cagacttaaa tagtgaaatc cctctcccca atattattgg caatatatat atataataat
    19681 catcaattaa gttatctttc ataaataatt ttactatatc gcctccacca actatccaaa
    19741 tatctttttt atccttctct tttccaattt gctcaattaa ttttttaggt tctaaattaa
    19801 tttatagtgt gattattcta tgtaaacatc acttttttac tcattcaata ataccctatt
    19861 aataaatcct caaactaatt taaataaact ttgcataaaa ttaaaaatat attatataaa
    19921 taatttaaaa ccacactaac ttattgaaaa ttctaattat tacaaataa
//
LOCUS       DAEMGG010000068        19919 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEMGG010000068 DAEMGG010000000
VERSION     DAEMGG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.068"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(96..1700)
                     /locus_tag="KPK24_002286"
     CDS             complement(96..1700)
                     /locus_tag="KPK24_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2299956.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVVDNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2086..3012
                     /locus_tag="KPK24_002287"
     CDS             2086..3012
                     /locus_tag="KPK24_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG2299957.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3132..4130)
                     /locus_tag="KPK24_002288"
     CDS             complement(3132..4130)
                     /locus_tag="KPK24_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG2299958.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4234..4671
                     /locus_tag="KPK24_002289"
     CDS             4234..4671
                     /locus_tag="KPK24_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2299959.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            4757..5344
                     /locus_tag="KPK24_002290"
     CDS             4757..5344
                     /locus_tag="KPK24_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG2299960.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(5644..6264)
                     /locus_tag="KPK24_002291"
     CDS             complement(5644..6264)
                     /locus_tag="KPK24_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBG2299961.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(6272..7039)
                     /locus_tag="KPK24_002292"
     CDS             complement(6272..7039)
                     /locus_tag="KPK24_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBG2299962.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7080..7901)
                     /locus_tag="KPK24_002293"
     CDS             complement(7080..7901)
                     /locus_tag="KPK24_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299963.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8260..10170)
                     /gene="phnW"
                     /locus_tag="KPK24_002294"
     CDS             complement(8260..10170)
                     /gene="phnW"
                     /locus_tag="KPK24_002294"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG2299964.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFETMLFETLE
                     DYTTPIPHVVETIEKLRENGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEELSKCKGIAKSLSLDVYAQWDTMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10449..11234)
                     /locus_tag="KPK24_002295"
     CDS             complement(10449..11234)
                     /locus_tag="KPK24_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG2299965.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKHSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11451..11681)
                     /gene="dltC"
                     /locus_tag="KPK24_002296"
     CDS             complement(11451..11681)
                     /gene="dltC"
                     /locus_tag="KPK24_002296"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG2299966.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(11700..12848)
                     /gene="dltB"
                     /locus_tag="KPK24_002297"
     CDS             complement(11700..12848)
                     /gene="dltB"
                     /locus_tag="KPK24_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG2299967.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(12848..14362)
                     /locus_tag="KPK24_002298"
     CDS             complement(12848..14362)
                     /locus_tag="KPK24_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG2299968.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14483..15673)
                     /gene="dltD"
                     /locus_tag="KPK24_002299"
     CDS             complement(14483..15673)
                     /gene="dltD"
                     /locus_tag="KPK24_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG2299969.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(15961..16980)
                     /locus_tag="KPK24_002300"
     CDS             complement(15961..16980)
                     /locus_tag="KPK24_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG2299970.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(17156..17695)
                     /locus_tag="KPK24_002301"
     CDS             complement(17156..17695)
                     /locus_tag="KPK24_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG2299971.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYRLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(17796..18476)
                     /locus_tag="KPK24_002302"
     CDS             complement(17796..18476)
                     /locus_tag="KPK24_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG2299972.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(18505..19794)
                     /locus_tag="KPK24_002303"
     CDS             complement(18505..19794)
                     /locus_tag="KPK24_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG2299973.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         6522 a         3469 c         2057 g         7871 t
ORIGIN      
        1 aatataattg ttgatttaac tgtaatctta tttaattcta cctaacttat tatataaaac
       61 tattttttat agattataaa atatctaact ttcatctact tcaaagaata caatacttct
      121 ccttctctta tagtctgcaa tactttaata tcttttattt tcatagggtc gattgtaagt
      181 gggttttcat ctaatattat caaattagct aatttccctt ctttaattga acctttttta
      241 tcttcttcaa aatattgata tgctgcattt atagtaacac cctttaaagc atctaatggg
      301 cttatctttt cattttctcc tatttgtata cctttttttg taattctatt gacagcacac
      361 catactgttt ccagcatatt aggtgctatt actggtgtat cctgatgaag agtgtaaatt
      421 aatccctttt ctatagttgt tttaagtggg ctaatcttaa acgctctatc ttctccaagg
      481 tttttgatat gaatatcacc ccaataatat gtgtgagcta caaagtatga aggtatcata
      541 tttattactt tcatatcatc aatctggtct gctcttactg tttgagcatg tatcataact
      601 ggtcttatat tattttcact actttgctct tttaagttta aaactttctc aaacgcatct
      661 atcaactggt ctgctgctgc atctccatta caatgagtta agagttgcat cttttctttt
      721 agtgaaatat ctataaactt ttctacttct tcatctttgt atataggata tccacaataa
      781 ccatcatctg aattttcata tggtctactc aaccatgctg tcttaccttg aggagaacca
      841 tctaagaaaa tcttataacc tcctatttta aatctattta tatatttttt tataaactcc
      901 ctattattat ctactacaga cttacttttt ttcaaattta catatcctac aacatccatt
      961 tttaatttat tttgatttgc cattgctttt aaaatgttaa attgattttc ttccattaaa
     1021 ccttcttgag ctgttgtaat accatagctt agatatatgt tctgtgcttt ttcaattgaa
     1081 ttaaaaatct catttgatga tggttgtttt attttagatg ctacattaaa aaatgcattt
     1141 tcttctaaat atccatttgg ttcttcactg ccttctactc tacctatatg tccaccttct
     1201 gggtcacgtg tttcattggt aacaccaagc tgttttaacc ccaatgtatt tgcaactccc
     1261 atatgaccag aagcatgtga aattagtatt ggattttctg aagatgcact atcgagtaca
     1321 ctcttatctg gatgcttatt ttcttctaaa aaattattat catatgaaaa tccaattatc
     1381 cagtctcctt tttttatatt attcgactct ttgaaatcct gtattttttt tactatgtct
     1441 ttaaaactgg ttgcatcttc taaaggaact agctttagag tagtagcaaa agctatcaag
     1501 tgactatgtg aatctataaa agatggcatc aatgtcttac cttgtaaatc tataatttct
     1561 gtatctttat ttttcaaagc aaatacttct tcttttgttc ctatcttttt tattattttg
     1621 tcttttataa gaatagcatc tccacatatt gaatcttcca ttgtgatgat atttccattg
     1681 taataaattt ttgctttcat aaaattctcc ttacgttgtc tactttaata aatttttttc
     1741 attattttct gcttaattat attatcttct ttataaactt tttctttaga gtttaatcat
     1801 ataaaagttt tatttttaat tattgtttgc caagaagtaa atactattca ttgtaaataa
     1861 gacaaattaa taggatttat actaattgaa aaaatattat aagttgttaa atttaaatat
     1921 ctaaactcaa ttatatctgt acaattgtaa ttttttaaat catgatgtat tattttaaat
     1981 atacagttac attattattc acaatataag tatcttagtt ctcaaataac atctcatgta
     2041 atctattcaa tgcaaaaaat ttaaatattt tttaggaggt tttttatgag caaggaaaat
     2101 aaaaatgtaa gaattgctgt tgtccaggcg tcaccagtca taatggattt agaaaaaact
     2161 gttgaaaaag cattaggatt aattaaagaa gctggtagaa aaggggcaaa tatcgtagtt
     2221 ttccctgaag cattcatacc tgcatatcca agaggtcttt catttggatt tgttgttgga
     2281 agtagaacaa tggaaggtag agaagattgg aaaagatact atgataattc tgtaccagtt
     2341 cctagtgcta caacagattt attaggaaaa gcagcacaag aagcaggagt atatctatca
     2401 atgggtataa cagaaagaga tggtaatgat ataaattgta ccctttattg cacaaactta
     2461 tttttctctc cagaaggtaa gctaattggt aaacacagaa aacttaaacc tacaggaaca
     2521 gaaagatgta tttggggaga aggagatgga agtactctta cagtagttga tactccatat
     2581 ggaaaaatgg gttcgttgat ttgttgggaa aattatatgc cattagctag aacagctctt
     2641 tatgctaagg gtgtaaaact ttacatagcc cctactgcag attcaagaga agagtggcaa
     2701 gcaactatga agcatattgc tttagaagga agatgttttg taattggatg caatcaatat
     2761 gttgaaaaaa atatgtaccc aactgacctt aactactata aggagctaga tgcagaacca
     2821 gaaattatgt gtccaggagg aagttgtata gttgaccctt ttggaaaata tgtagcaggt
     2881 cctattttca ataaagaaga aatgctaata gctgatttag acctagaaaa aatagtactt
     2941 agtagactag attttgattc agaaggtcac tattcaagac cagatgtatt tgaattaatt
     3001 gttcatgaat aatcttttta cacaaacatc tacataaatc tttttacata aaaaaatgag
     3061 atactcataa gtaaatttca agtatctcat ttttgcttaa aacctataat cctaattata
     3121 aatttattca cctatatttt ttctaaatca actactgttc ttccaacatg ttttcctgcc
     3181 attattgaat ctatactatc atttaactct tccaatgata ctacatttac catatcatct
     3241 aaatcatcta atttccattc atttgataat aaatcccata tttttaatct taagttcatt
     3301 ggacaatcca cagaatctat accatataaa gtaattccct ttaagataaa tggatatact
     3361 gaacttgtaa aatcaactcc tcctgcatta ccacaagttg ttactgctcc acaataatta
     3421 gttgtcttta ttgctgttgc tagagtattt cctcctactg tatctattac tccagcccat
     3481 cttggtctaa gcagaggtct tcctgagtta tcatccactt ctgacctatg tataacatct
     3541 ttaacaccta agtttagtaa tacttctttt tgttcttcta attttcctgt tgaaactaca
     3601 actgaataac ctaatttaat caatatttta gttgcaatta cagcaactcc tccagtacca
     3661 ccagtgacta atacatctcc catattcttg tctacagaat ctataaattt atacacagat
     3721 aaagctgctg taaaccctgc tgtaccataa tccattgctt cttttagtga aagattttta
     3781 ggaagtttaa ctacccaact agaaggaact cttatgtatt ctgaaaaacc accagaagta
     3841 ttcattccca agtcataacc tgttactaaa acttcttctc caattttaaa atcatcacaa
     3901 ctactagatt ctactatacc agatgcatca attcctggtg tatgaggata ctttctcgta
     3961 acacccttat taccatttgc agaaagtgca tccttgtaat ttaaagaaga atattttact
     4021 tttattaaaa cttcgccttc tggcaatgta tccacttctc tttcaactat atttcttcta
     4081 aaatttcctt cttcttctgt aatatatagt gatttaaatt ttttattcat aaatttcctc
     4141 ccaatacatt tagttttatg ttaaactaat tatagtttat aaatagtttt atgtcaaact
     4201 aatttaaata gattaaagat tggagaataa tttatggaag ataaatatat tgtacatttc
     4261 ataagtaaaa caaaggctag tatgattaaa ttcattgaaa ataaactcag taaaaatggt
     4321 ttaggtgagt taatacctac tcatggaaat attttaactg cactttatga aaataatgga
     4381 atacttacaa tgaaagaaat tgcagctaaa ataggaaaag ataaatccac tgttacagtg
     4441 ttggttaata aacttataaa cctaggatat ttagaaagac aaaaatgtac aaatgacaaa
     4501 cgtatcacgt atataaagct tacaaaaaag gcactgctta tagaagatac ttttaatagc
     4561 atctcaacac aagttaaaga gacggcatat cataatatta cagaaaatga aaaacaagaa
     4621 tttcttagaa ttttaaaaaa aattaatgat aattttaaaa atgctgacta aattgagcat
     4681 aattatatct cctattggaa taataaataa aaagaatcaa atattgtaaa aatttattgt
     4741 gatagagagg tagaatatga atttaaaaga tagtaaaact aaagaaaatt tattaagagc
     4801 ttttgcaggt gagagtcaag ctagaaatag atacaatatt tctgcatctg tagcaaaaaa
     4861 agaaggatta tatataatag agtctttatt taattacact gctgaccaag aaaaatcaca
     4921 tgcaaaacaa ttcttttcta aattaaaaga atttgctggt gaaaatataa gtatatcagg
     4981 agcataccct gtagatttat atgacaagac agcacaacac cttaaagctg cacaacataa
     5041 tgagcttcaa gagtgggatg atgtatataa aaactttgga aaaacagcac gtgaagaagg
     5101 atttgaatct atagctaagt tatttgaaaa tatagctgaa atagaaaaag ttcatggtga
     5161 tagatttggt agatatgcta ctgatttaga agaaggtact ttattcagaa gaaatgaaga
     5221 tatacaatgg atatgtacac attgtggcca tatacatata ggtaaagagg ctccaaaagc
     5281 ttgtcctgta tgtcttcatc cacaaggata tttcttagat tttaaaaact cattatttga
     5341 ataaactaag gtaatcattg tatttaaaca atttactata tttatatcaa gtatttttat
     5401 aaatactgta ttatcttaat aacaataaaa tgaaccattg atattactat aagtagaaaa
     5461 tgtagtttaa atacaattta tagtagttca tcatggttca ttttattgtt tactaatccc
     5521 tataacctac ttaaatctat ctttaacttt tacacactca cataaaatct catctttaaa
     5581 ctaaatattt aaataaagtg tattttaagt atattattat aatacattat ataagataat
     5641 acactatgat aactttatac ttatatcatt tatttctttt gtcaagtcaa cctcaataat
     5701 cttaactaca tctttttcaa actttacttt tttataatcc aaacatccta ctacgaatgg
     5761 aactccaaac tttctaaaca aataaatctt tccactttca ttgtatattg atgtaaaatt
     5821 ttcttctatt atattcacta tcaattttaa ctcttcaaaa gttattatag atttaactaa
     5881 aaacttctct attatgctca atataagcag ttctttttta cctatgcttt gctctttttt
     5941 atataaattt tgaaatattt ctttggtata attacatata acttctttac tgagaataaa
     6001 atcaatgtca aattctttat taatatcctt tttcataaac atatttgcaa tatcatcaag
     6061 agaagtctct tctttcatag acaatattaa ctctattctt tctagtattt tatctcttgg
     6121 aaagaaagtc tcttgacctg taaaagctga tttttttata aaccattcct ctggtattat
     6181 gttttttctt ttccatctat ataattgacc ataagatata ttagttttgc tcaataaatc
     6241 tttcttagat attaattctt ccataacttt tctagcccac ctttatactg ttttttacag
     6301 tagaattatc tgaaactgat aaattatcac tgtattcaat atttccaacg tcacaattat
     6361 ctactagaga tatgcttttg cctcgtatat cttttattga tgcatcttct aattcaatag
     6421 tatctccctc tatcaaatta catttaaact ttcctgaaaa aatattaatg attttgatta
     6481 gtgaaaaagc atctttgctt cttcctattt ttatattagt tgcccctatc tctttacaat
     6541 aagaacctcc tcctgaataa acatatatat tttctccatt taaaagacca tcacatttta
     6601 ttactccata tacctttata tcttcacatt ctatatttga tttatttctc atttcgccat
     6661 agatttttat atcttgtgca tacatatttt catttacact taattgtcct cttattgtaa
     6721 cattattaca tttaagtttg ccatccactt tctgttgagc agttataact aagtcatcta
     6781 tttctgcatc tccctctata ttaagttgac cattcactct tacacgctct gctttaagat
     6841 tgcctcttat aatagttttt ccgttaacaa caaaattttt tatattgatg tcaccttcaa
     6901 attttgcatc tccaaggact tttacttcgt tacttttaac atttcctttt gatttagaac
     6961 ttcccacgat ttttatattt tcatactcac cactacttat aaagccttct cctagtattt
     7021 ttatagaccc tttttccata ttattacctc attttaaatt ttatttttaa acctatttat
     7081 tacttatcct tctttaattt tattttttca cttatattct ttatttcacc aactattgaa
     7141 tcttcatcaa tctctatact ttcactgtac tccaaacttt ctacataaca tcccttgcct
     7201 attttaatat tttttcctct tacaactttg acttcactat tttctaagct tatttcatct
     7261 gcttctataa tatttgcaaa caatttactt tctgtatact ttctaattga taatagacct
     7321 aaacttatca aatttgaaga cactttttca aagttgatat gctttttttc tagcatacga
     7381 aattttttgc aataatctcc atgccccttt tttatattta tactacttgc tccaacttca
     7441 ttaaaattag aagttccatt taaactaata ttaacttctt cacaattcaa aaatccatta
     7501 cactccaaga ttccacttaa attgatattt tcacaatcta tattctcatt tgcttttaaa
     7561 tatccttctc catgaataat atcagatttt atacttcctt ctacattaat aattccacta
     7621 gtatcaattt caacagaatt aatatttcct gtacatctaa gaagtcccga tatctcaact
     7681 tttgatgcca gtatgtttcc ttctacttta cttaaaccag atgtaaaaaa ttcattacat
     7741 tctgttgtcc caaaaacttt tgctgaacca gaagaatcaa atttactaca ttttacactt
     7801 ccttctattt tgcctgttcc tgatatatta acattatcaa aaattccacc tttacatttt
     7861 ccaacaccat caaatcttag tgaacttaat tcttcattca taatgatatc ctttctttac
     7921 cgtattttat ctttaaacct aatataacaa aacattgtta cgttgtcaag tatgataata
     7981 atacttaata aagcaactat catttatttt ctataaatag gcataaaaat aatagggaaa
     8041 tgctttattt aaactataaa tctaatttct tagattgtgg tttaaataaa atatatccct
     8101 attactcaat ctttatatat tcattgtata aacttctaaa atataagtct ttaaattcta
     8161 ctgtcaatac tttttataca ctgtttgtgc tatcatctca cacacctaaa ggagtgggct
     8221 ttccgcacta ttctgtaacc tacaagacac tatcttcttc tatctattga taaatttttc
     8281 tattacatca gctagtctct ccatatcagt tggataaact tcacctatgc tacctattct
     8341 aaatgtatct atatcagtca atttacctgg atagataaca aatccattat cttttaaata
     8401 agtataaaat tccataaatt caaactttgc atttttagga tataagaaag ttgttattac
     8461 aggtgattga gcattatcat tcaccaaagt atcaaagcct aaagatttta atcttgaagc
     8521 tattgtaaac tgattttctt tatatctagc atatcttttt tcaacagaac cttcttcttc
     8581 caattctaat aaagcttgat aaaaagctct tacaacatgt gtaggtgaag taaatctcca
     8641 tttaccatta tttttctcca tagtatccca ttgcgcatac acatcaagag ataatgattt
     8701 agctatacct ttacatttag ataattcctc tttattagct attataaaac caaatccagg
     8761 tactccttgt atacatttgt tagaactact aactaaaaaa tctatattaa aatcttcaac
     8821 atcaatctca attcccccaa aactactcat agcatcaact atataaattt tgttatattc
     8881 ttttgcaagt ttccctacct cttgtatagg atttaatcta cctgtagtag tttcacagtg
     8941 aaccattgat atatgtgtta tatccttgtt ttcttttaat aaattttcta ctacatttaa
     9001 atccactgct tctatatctt taaatgtgca gtcaacaaat tctatgttaa gataattaca
     9061 tatatctttc attctctttc catatgctcc atttgcaatt acaagaagtt taccatcttt
     9121 tgaaatagta cttcctatta tagcttctac tgaaaaagtc ccacttcctt gcattaatac
     9181 acttgtgtat ttatctgtat tttgggttgc tagagatact aatcttcttc taacatcttg
     9241 tactaggtta ttatattcaa catcccaagt acaccaatct tttaacatag atgctcttac
     9301 tgatttagta gtagatagtg gtcctggtgt aagtaatatg taatcatttt ctggtacaaa
     9361 atctgattta atagtttcat tctctatttt tattagtata tcttctaact cagccatagt
     9421 ttctattaca aaatgtgcac ctgcctcttt aaatttcttt gaaactatac tcatcttagc
     9481 ttttaattct tccttatcca tgttctctac ttcttcttgt gtaagcccta actcacttga
     9541 accttttatt acagcaacac tccacattcc tgcatttacg ccttctttta catcacttat
     9601 tgtatctcct acttttacca tagaactcat aggagacacc cctaaagctt cagcattttt
     9661 atagcacatc caaggatacg gtcttccttg agatacttct gatggtgtta caagaaaatc
     9721 gggtgagtat cctttctttg ctgcatttgg ttcaactatg ttcatcatct ctcttgtata
     9781 acccgttgta gaacctattt ttaatccatt ttctctcaac ttttcaatag tttcaacaac
     9841 atgaggaatt ggagtcgtat aatcttctaa tgtctcaaat aacattgtct caaattcagc
     9901 ataaagttca tttacatcat cttcagttgg cactttacca aacttatctg accaaagatt
     9961 ttttatccta tccatttcac acatttctct tatatggtct atttttaatt ttcccatagg
    10021 ttttcttgct tcttccattg ttacatctat acctcttctt ttaaatatct ctataaatac
    10081 attaagaggt gcaaaacatc cataatctac tgttgttcca gcccaatcga atactactgc
    10141 ttttattttt tctccatata tctttttcat atttatagcc tccatagatt ctttttagaa
    10201 tttttgtgtt tttaattgtt tttactttta aagtataact ttcatatgtt aattatacat
    10261 catatttagg ttaaatatat attaaatttc tgtttttagt tgtatttagt tgtttttatt
    10321 ttgtatcaca ttcccataaa atcctaagtt acaatctaaa atttactttc taatcttaat
    10381 ctatgaactg actatatttt atgaatttag ttgtattgag catagttgta ttatataaaa
    10441 ctaagggttt attttgcttc tatccatgtt atatttatct catttaactt atccttccat
    10501 tcattcggag gttctacatc acaaataaca aaatctaatt cctctaaatc tgctatctta
    10561 tacatactag caattcctat tttagaacta tcacataata caaaactttc ctttgaattt
    10621 tttatatatt cttttgacaa caatgcctta ttttggtcta tactactaat tccaaaacta
    10681 tcaagtatac cttcactaga tataaaagct ttatctacgt ataagttttt catagtatct
    10741 attgacattg gacatgctgt cctttggtgt tttaaattta cttttccacc aataaatata
    10801 acttctccat caaacaaacc ttgattctca taagaaatca attttgatag aactggataa
    10861 gagtttgtaa taatttttaa attgtttaca gatattatat aatctacaat ctgcaaattg
    10921 gtagtacctt catctatgat aattgactca ttgtcattta caaatttagc tgcatattta
    10981 gcaatactat gctttttatc taggttagta ttgtttctct ttatatgttc tggttcaatc
    11041 actccataat ttatttttat agcaccacca tatacctttt ttaatttatt ttcattttct
    11101 aattcttcca aatatcttct tattgtttct gatgatactt ctaatctttt tactaattct
    11161 ttagtgttta ccttcccttt tgaatctaat aatttaagta tttctttttt tctatctatc
    11221 gaatttaaag acattcattt tcctcctaaa tataatatat ttttctccta taatattaca
    11281 atgataccta agatttatga aatcatattt atgtattaaa ttttttactt aattaaagtt
    11341 attttcaaaa gactttgtat tatcattaaa aaagtccatc aaatattagt atcagatgga
    11401 ctttgtaaaa ttttatttta taaattaata tttaaagtct aactactaat ttattttctt
    11461 gtctccaaga attttactaa attatttaca gtagacatat cttttctctc taaatcagtt
    11521 ggttgaagct ctatccctaa tctattttca atttcaagta aaacttcaat tatagctaat
    11581 gagtctaaaa gttccgtctc aaataaatta aggtctaaat cttctcttat ctcatcagtt
    11641 cctaatacat cttcaaatat ttctataact gtttcttgca taaattttcc tccttaaaat
    11701 taagctaaat aacctgagaa tatcagcaat ccaaaacaca caatatggaa tgtaatgaaa
    11761 atctgcactc tctcgaacca tttatctttt ttatgttttt tataaaattt agattttcgc
    11821 tgatatatat cagttaacac aagtgccaat ccttggtata caccataagc aacataatac
    11881 caagttagac catgccaaaa tcccattgtt atcatggtta tcatctgagc cacatgagca
    11941 gcagtagtcc ttttcttaaa tcttttcttt ctcattgatg acataacaaa tctagaaaaa
    12001 atataatctc caaaccatct tgaaagactt atatgccatc ttgtccagaa ctctttcata
    12061 tctttgctga taaatggctt gtcaaagttt ataggtactt gtattccaaa tatgtaccct
    12121 gtacctatag caaataaact atatcccgca aaatcaaaga acaggtataa actatatgcg
    12181 tacatataac ttaaaatatt tataaaactc atatctttag gtattcttga tacccaatat
    12241 gtatttatca aaaatgctat aacaaactta taacctactc ccatgacaat attttttatt
    12301 cctggcagta agtattcttc tatatactct tttctagaga tttgtttttc caagtcctgt
    12361 tcaaatcttc ttgacctatc aataggtcca gaacttaaag ttggaaaaaa taacataaaa
    12421 taaagcatct ttgaaatttt aacttctttt atagctccat cataaatttc aataaccatt
    12481 tgtatagttc tgaaatttaa gtatgatatt ccaataaatc caattattcc aaaagaagta
    12541 actggagata ttttatttat tattattggg agcattgatg caaacaagaa tccccaatat
    12601 atgtatttat ttttagtttt tcttcttaca tattcatatc ctttaactat tattacttca
    12661 agaataataa atattaccaa gtactttagt tggacatcaa ttcctactat taagaatatc
    12721 ataaacaaac ttgccaacat accataatac tttatattct tgcccattag acctaaaatt
    12781 actgctggta ttgatgttaa taataatata taaagataaa aataatctcc atattgtgaa
    12841 aaaatcatta aatttcctcc ataagtttct ttctgtctat tttaccattg atatttgttg
    12901 gaaactcaga gattattgaa atatttcttg gaatcatata tgaaggtata tactttccaa
    12961 gttccttttt tattatcatc ccatttttaa tattacttaa atcattcttt tcatttaact
    13021 caacaatacc ttttaagtaa gcaatttttt cgtccttata tactggtaaa actactgcat
    13081 tttttacatt atgtactttt ctcaaattat tttctatatc ttctatttca attctaaatc
    13141 catttaattt aatctgaaaa tcttttcttc cacaataata tatatttcca tctaaaagat
    13201 atcccatatc tccagtttta taagctctcc actttacacc atctatttca tcatagaaaa
    13261 atacttcatc agtcttttct ttattattga agtaaccttt agaaactgat ggtccaatta
    13321 ttataatttc acccttttca ttttctttaa gctcatttcc atcttcatca agtattttta
    13381 ttttacaatt tgacataggg tatccaactg gaagactttt ttcatcatct atagcctttt
    13441 gagtcatatc atttacactt acaccaacag tagcctcggt tggtccatat ccattgataa
    13501 ttcttgtgtt tggaaatcta ctcataagtt ctttagtaag attttttgaa agtgcttctc
    13561 ctatgaatat cattgactca aggttaggaa gcattttact attaaactct ttttcagtta
    13621 tacatactcc tgcaaaagaa ggagttgaca cccatacagc tatatctgag atactaaatt
    13681 gtctaaatag ttctttatag tctgctaata catcttttga tattgaaaat aatgtagctc
    13741 catggataag accagggtat attgttgtaa ctgaaaggtc aaaagaatat gctggttggt
    13801 tcattattac tttttcactt ccatctatgt ttagatatgg gcttatccaa tctgaaaagc
    13861 tatctaaatt gtttgaactt atttgaaccc cttttggctt tcctgtactt cctgaagtaa
    13921 atagtatata tgcattttca tcatctttta cccagttttc tttatctaat gattttccct
    13981 gatattcatt tattatataa tttaatttat ccatatcaat tacattgata tctccaaaat
    14041 ttctttcatc actaaagtta aataatacct ttggttttat ctctttagtg acttcaaaaa
    14101 ctctatcaat tggaaaacta atatcaagag gcacatacgc tctacctgat tttaaagctc
    14161 ctatcataca tgccattatc atgttttctt tatttccata aattactata ggagtatctt
    14221 cttctttgta tacatccttt agaaatactg atatagcatc tgaatattca tttaaatctt
    14281 tataagaaag tttatctcca ttacacatta gtgctgttct atctgtattt gaatattttt
    14341 ttattccttc aataattttc atataagaat gctcctttaa aatccgttat aaataaatgg
    14401 caattcttta aatggagtaa atgttagaac tcctatcgcc aatattataa ccatagttaa
    14461 acaaatacta gttaacaaat ctttattgtt ctttaaatat tttaaataat ctctcacaga
    14521 catctaccca tcctttcgta ccaagatgca ttacatccct taaataatac ttatctgacc
    14581 ctttatcttt aaggtttaga acttcaaagc ctttgctttc tgctattttt tgtgctttat
    14641 cgtagtactg atttctttct ttttcagaaa taccagtcaa attatagaat ttatccatac
    14701 ttgggattaa tacaacaact ggcttaatac ctaagtcagt acatacattt agcatcagtt
    14761 tataattttc aaactcctta gatgttaata aatttacatc tttgtctctt cctttgtatt
    14821 gctctatacc atctttaaaa tgctctttat aatagtactt atcaatattt agtggatttt
    14881 taccaactct ttttttagca tcttcaatgg ctttctttct ctcatatgac caatttattg
    14941 gtctttttct ttttgttgct ctctttttat ctagcttaat taatcgttta taaagaatac
    15001 ccttttcttt aagagctata cagtactttc ttccttgaaa ataaggctct aaaagcacct
    15061 tttcagcttt ttcaggaaat gtcttaggct catatagctt agcatataat gcttctgctt
    15121 tatactcatc acttccccat aataacttac ttgatttttt tgcatattca atcttatttt
    15181 gtttagatat ttttggatta tctaaaaatc gataaaattg tattggtgaa aatctagact
    15241 gataatggtg acttgtgact ccgtcttttt ccataaacca ttgcatagaa attaacaaga
    15301 caactttctt attatcaata ttaggattca tacttcctaa tattgcagca tcttgtaatg
    15361 tctgagtata ggctctccca attgcaaaca ccttattttt actcctattt gtattaaaat
    15421 agtactttgg atgttgtttt gttgaattac taagttctga tgaccccaaa atcataatat
    15481 ccttttctct taaaaaatga ttatttgctg taaccccttt atcttttata tcacttaaag
    15541 tatcatccat aattggaagt agatttttct ccctcaaaag ttcatcagtt ttagaatcaa
    15601 gaaatttgtc taacccaaat aaaaatacta cgcctattat aaaaggtgta ataaagtata
    15661 tcaattttct catattttct ctcctctaaa aatattcaaa tttatgttat tattaatcat
    15721 attagttttg atatgtatat tcaactacaa aattgtaata aaatcgtcaa aacttttttt
    15781 gatactgtta caaaattgta aaaaaaaaga aaaaaacgcc aaaaataaat ttctttttag
    15841 cactttttca tacttataat ttgacatttt aacacttggc taagtataca ccttacctta
    15901 acttaaaatt aaatttattt aagaatatat attataaatt cttatatttt ctaatataaa
    15961 ctatatacta tcagctggat aaacaatact actttgtctt cttatctcat ccataatttc
    16021 cattactatt attgaatttt catgagaatt tatatttgat tctattttat tgttatttat
    16081 aagacttata aactctacaa gttcataata cattccttct atttctctag gcttttcctc
    16141 tgctttttct tttggaatag atatatcttc ttctcttcca tctctgtatt taataatagc
    16201 tttttcaaat agattaagct tttcaattat tatacttccc tcttctcctt gtatttcaga
    16261 tggtatataa gaatcagcta ctttagaata ctgaattatc gcatccatat catcatattg
    16321 aaatacagct gaaccttctc catccactcc agtttgaagt atatgagaaa ctgcctttac
    16381 agtttttgga gcgccaaata aattcaccat aggatgtata caatacactc ctatatccat
    16441 taaagctcca tttgataact cttttttaaa ggcattttct ataattccat ttttatattt
    16501 atcatatctt gaagaatact gacaatagct tccaaaaaat cttcttattt tccctatttt
    16561 atataagttt tctttaactg ctttaaagtt tggaacacat gtaattctca tagcttccat
    16621 aagcaataca ttattatctt tggctgtttt tatcattgat ttgacttctt ttaaattaga
    16681 tgctaacgac ttttcacaaa gcacatgttt tttgtgtttt aaacaagtaa tggtttgctt
    16741 tgaatgtaat gcatttggag aagctatgta tacagcatct atatccttcg atttagccat
    16801 ttgttctaaa tcatcaaaaa aaatacttgc tccatataga gttccaaatt ctctagcttt
    16861 ttctaaattt ctagaatata cagcaaccaa ttcaaagtct ttaaccctag atgcagctct
    16921 tagaaatata tttgctatat tgcttgtccc aacaatacca aatcttattt tacccatcat
    16981 aaatcactcc ccattttcta cactttattt taattttaat aaaaaacttt acaacttata
    17041 gatatattat aaaataattt tatcaaataa aaaagaaagc taattttaaa tgatttcacc
    17101 attttaacat agctttcttt taactacaat ctaaattcaa cttatatttt taaagttatc
    17161 tttctactat atctttattt cccctcaaaa attttatagt aagaatttca tccacaaaca
    17221 atgttactaa aagtatagtt acaacaattg gccaataagg tattataagt ctatataagc
    17281 tatcaactat atacacttga aaatatctaa ctccaataaa tgtcaatata gtccaatata
    17341 cagaaaactt tagacatact attcctctac tattgtactt ataatctgaa taatcccaat
    17401 agaccttatt aaaatatact ttcataagta ttcctgtaat atattctact actgtcggca
    17461 ctactagaca taataaaagc aatgcaaata tattattggg gtcaataaat cttgagacct
    17521 ctattataat cgacattgca attgcataca ttggtttaaa tggaccattt aaaaatccat
    17581 cttcttgaaa atgcccttgt ttataataac tatataagtt ttcaattatc caacctataa
    17641 aaccatacaa gaaaaaatta aataaaaaat ataatagaga acctacatta tccatgaata
    17701 ttcctcctag ctgtttatac ttattttttc tcatattcat ggatttattc taaatcaaag
    17761 aaattatatt taatttattt aattaatact ttatattata gattgtgaca ttctttagag
    17821 ttcaaacttt ttaggtattc tctaacttcc agtaaatttt caagtatctt atgtgattgt
    17881 gatttaattt gctcatctgg ctcttttaaa tctggtacat ttatacatct tataccacca
    17941 ttgtatgcag cttcgactcc cataggcgaa tcctctatga caatacaatt tttgggatta
    18001 acatttatct ttttagctgc ttttaggaat atctcagggt ttggtttaga gtttacaaca
    18061 tcatctccac aaactatagc atcaaagtaa tcttttaaat ttgccttagc taatctcttt
    18121 actgctcttt ctctctttgt tgatgttgca acagccattt tgtatccatt ttcttttaaa
    18181 aaagagatta gttcattgac tcctaatttg ataggagctc ccttcctctc cataaattca
    18241 atcatgtttt tagttttttc gtcatataaa tctattattg gtacagaact atcgtatata
    18301 tctgtaagac cttctattat tcctttacga tttcttccca taactgatgt ataaatttcc
    18361 ttagtcatag tatatccata cttttcaaac gtctccatcc aaaattcaag agaaattctc
    18421 tctgagtcaa acaaaactcc atccatgtca aaaataattc cttctacttt ttgcatacaa
    18481 ttcctccaat attttttgat ataattatat tacacttttt atgattaatt ttacaaccct
    18541 caatttatcc aattctaata ttgtaaaatt gtaattttca aaagttacac tttggtttac
    18601 tttaatattt gaaccaagca tataggatat ccacccacct atactatcaa atccatcatg
    18661 ctctatatca agtccaaaat acttattaat atagttgata gattcagttc catcaactaa
    18721 gtaagaacct gctcctattt tcttaatatg agtctcttct ttgtcaaatt catcttgaat
    18781 ttctccaact atctcttcta gtatatcttc tattgttacc acaccagatg taccaccata
    18841 ttcatccaca actatagcaa gttgaagttt ttcctttctt attctctcaa gagctttatc
    18901 tatagttaaa ttttcagata tatatataat atctctcaat atttcttcta gttcaatttt
    18961 attttcattt atcttttgat tgtataaatc tcttatatgt acaaaaccta atatatcatc
    19021 tttatttttt ctgcatactg gatatcttgt aacaccttcc tcttttaaaa tagctaatat
    19081 cttttcttca gaatcacttt catatataca taccatatca gttctaggaa ccattatctc
    19141 tctaattttc ttttcttcaa attcaaaaat attatcaact aatctttgct ctgattcatc
    19201 tataagtcca cttttataac tttcttctac taatagtctt atttcgtcac ctgtatgggg
    19261 ttcatcaacc tcatcagctt gtgaatatcc aaatggttta agaaataaat cagtacttaa
    19321 gttgaaaaaa tatattattg gatatgttaa cttataaaat cctactaata aaaaagatgt
    19381 actcaacatt attttttctg tattataaag tgctaaagct ttaggaacca actctcctat
    19441 tacaacctcc atcattgtta taattaaaaa agaaatggaa attgatatag tatatataat
    19501 actttcacta agattaaata cacttactat tggtaaaatt aactctttaa ctgtagattc
    19561 tcccatccaa cctaatgcaa gtgaacaaag tgttatacca agttgacatg cagaaagata
    19621 ggaatttaag ttgtctttta ttattttaca tctttttgca ttattattac cttcagttat
    19681 catggtatca atcctagaac tacgaagttt taccatagca aattcacaag ctacaaaaaa
    19741 agcatttatc aaaacaagca ttactataag tgttactttt aataactccc gcataaagta
    19801 ttggttctta taatgtaaga aatctataag aactcaaagt tcccccctta tatttataaa
    19861 tttaatttat aatatatatt ctatcatatt tattaatata tcctacttta ttacaaata
//
LOCUS       DAEMGG010000069        19904 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEMGG010000069 DAEMGG010000000
VERSION     DAEMGG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.069"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..137
                     /locus_tag="KPK24_002304"
     CDS             <1..137
                     /locus_tag="KPK24_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2299974.1"
                     /translation="YKYLKGYSDREISVKLKISRQAVNKAKNRAFKKIKKDYENYFNL
                     "
     gene            455..7555
                     /gene="tcdB"
                     /locus_tag="KPK24_002305"
                     /gene_synonym="toxB"
     CDS             455..7555
                     /gene="tcdB"
                     /locus_tag="KPK24_002305"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG2299975.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            7677..8177
                     /gene="tcdE"
                     /locus_tag="KPK24_002306"
     CDS             7677..8177
                     /gene="tcdE"
                     /locus_tag="KPK24_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG2299976.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8506..8688
                     /locus_tag="KPK24_002307"
     CDS             8506..8688
                     /locus_tag="KPK24_002307"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2299977.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            8905..17037
                     /gene="tcdA"
                     /locus_tag="KPK24_002308"
                     /gene_synonym="toxA"
     CDS             8905..17037
                     /gene="tcdA"
                     /locus_tag="KPK24_002308"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG2299978.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17372..18070)
                     /gene="tcdC"
                     /locus_tag="KPK24_002309"
     CDS             complement(17372..18070)
                     /gene="tcdC"
                     /locus_tag="KPK24_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBG2299979.1"
                     /translation="MFSKKNDGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            18496..18741
                     /locus_tag="KPK24_002310"
     CDS             18496..18741
                     /locus_tag="KPK24_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG2299980.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLI
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(18959..19228)
                     /locus_tag="KPK24_002311"
     CDS             complement(18959..19228)
                     /locus_tag="KPK24_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2299981.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT         7882 a         2258 c         3016 g         6748 t
ORIGIN      
        1 tttataaata tttaaaagga tattctgata gagaaatatc agtgaaatta aaaatatcta
       61 gacaagctgt taataaggct aaaaatagag catttaaaaa aataaaaaaa gactatgaaa
      121 attattttaa cttgtaatta atgagcttaa agaaatattt acaatagaaa tcaaatttta
      181 gaattaactt tattgtaaaa tcaataactt aatctaagaa tatcttaatt tttatatttt
      241 atatagaaca aagtttacat atttatttca gacaacgtct ttattcaatc gaagagcaaa
      301 ttaatcaact gagtgtcttc aatttaaaat gttaggaagt gaatgtatat gaaaacctaa
      361 gtagatatta gtatatttta taaatagaaa ggaggatata taaaagagtt ttatcattta
      421 gatgtaaaaa tattatagta aaggagaaaa ttttatgagt ttagttaata gaaaacagtt
      481 agaaaaaatg gcaaatgtaa gatttcgtac tcaagaagat gaatatgttg caatattgga
      541 tgctttagaa gaatatcata atatgtcaga gaatactgta gtcgaaaaat atttaaaatt
      601 aaaagatata aatagtttaa cagatattta tatagataca tataaaaaat ctggtagaaa
      661 taaagcctta aaaaaattta aggaatatct agttacagaa gtattagagc taaagaataa
      721 taatttaact ccagttgaga aaaatttaca ttttgtttgg attggaggtc aaataaatga
      781 cactgctatt aattatataa atcaatggaa agatgtaaat agtgattata atgttaatgt
      841 tttttatgat agtaatgcat ttttgataaa cacattgaaa aaaactgtag tagaatcagc
      901 aataaatgat acacttgaat catttagaga aaacttaaat gaccctagat ttgactataa
      961 taaattcttc agaaaacgta tggaaataat ttatgataaa cagaaaaatt tcataaacta
     1021 ctataaagct caaagagaag aaaatcctga acttataatt gatgatattg taaagacata
     1081 tctttcaaat gagtattcaa aggagataga tgaacttaat acctatattg aagaatcctt
     1141 aaataaaatt acacagaata gtggaaatga tgttagaaac tttgaagaat ttaaaaatgg
     1201 agagtcattc aacttatatg aacaagagtt ggtagaaagg tggaatttag ctgctgcttc
     1261 tgacatatta agaatatctg cattaaaaga aattggtggt atgtatttag atgttgatat
     1321 gttaccagga atacaaccag acttatttga gtctatagag aaacctagtt cagtaacagt
     1381 ggatttttgg gaaatgacaa agttagaagc tataatgaaa tacaaagaat atataccaga
     1441 atatacctca gaacattttg acatgttaga cgaagaagtt caaagtagtt ttgaatctgt
     1501 tctagcttct aagtcagata aatcagaaat attctcatca cttggtgata tggaggcatc
     1561 accactagaa gttaaaattg catttaatag taagggtatt ataaatcaag ggctaatttc
     1621 tgtgaaagac tcatattgta gcaatttaat agtaaaacaa atcgagaata gatataaaat
     1681 attgaataat agtttaaatc cagctattag cgaggataat gattttaata ctacaacgaa
     1741 tacctttatt gatagtataa tggctgaagc taatgcagat aatggtagat ttatgatgga
     1801 actaggaaag tatttaagag ttggtttctt cccagatgtt aaaactacta ttaacttaag
     1861 tggccctgaa gcatatgcgg cagcttatca agatttatta atgtttaaag aaggcagtat
     1921 gaatatccat ttgatagaag ctgatttaag aaactttgaa atctctaaaa ctaatatttc
     1981 tcaatcaact gaacaagaaa tggctagctt atggtcattt gacgatgcaa gagctaaagc
     2041 tcaatttgaa gaatataaaa ggaattattt tgaaggttct cttggtgaag atgataatct
     2101 tgatttttct caaaatatag tagttgacaa ggagtatctt ttagaaaaaa tatcttcatt
     2161 agcaagaagt tcagagagag gatatataca ctatattgtt cagttacaag gagataaaat
     2221 tagttatgaa gcagcatgta acttatttgc aaagactcct tatgatagtg tactatttca
     2281 gaaaaatata gaagattcag aaattgcata ttattataat cctggagatg gtgaaataca
     2341 agaaatagac aagtataaaa ttccaagtat aatttctgat agacctaaga ttaaattaac
     2401 atttattggt catggtaaag atgaatttaa tactgatata tttgcaggtt ttgatgtaga
     2461 ttcattatcc acagaaatag aagcagcaat agatttagct aaagaggata tttctcctaa
     2521 gtcaatagaa ataaatttat taggatgtaa tatgtttagc tactctatca acgtagagga
     2581 gacttatcct ggaaaattat tacttaaagt taaagataaa atatcagaat taatgccatc
     2641 tataagtcaa gactctatta tagtaagtgc aaatcaatat gaagttagaa taaatagtga
     2701 aggaagaaga gaattattgg atcattctgg tgaatggata aataaagaag aaagtattat
     2761 aaaggatatt tcatcaaaag aatatatatc atttaatcct aaagaaaata aaattacagt
     2821 aaaatctaaa aatttacctg agctatctac attattacaa gaaattagaa ataattctaa
     2881 ttcaagtgat attgaactag aagaaaaagt aatgttaaca gaatgtgaga taaatgttat
     2941 ttcaaatata gatacgcaaa ttgttgagga aaggattgaa gaagctaaga atttaacttc
     3001 tgactctatt aattatataa aagatgaatt taaactaata gaatctattt ctgatgcact
     3061 atgtgactta aaacaacaga atgaattaga agattctcat tttatatctt ttgaggacat
     3121 atcagagact gatgagggat ttagtataag atttattaat aaagaaactg gagaatctat
     3181 atttgtagaa actgaaaaaa caatattctc tgaatatgct aatcatataa ctgaagagat
     3241 ttctaagata aaaggtacta tatttgatac tgtaaatggt aagttagtaa aaaaagtaaa
     3301 tttagatact acacacgaag taaatacttt aaatgctgca ttttttatac aatcattaat
     3361 agaatataat agttctaaag aatctcttag taatttaagt gtagcaatga aagtccaagt
     3421 ttacgctcaa ttatttagta ctggtttaaa tactattaca gatgcagcca aagttgttga
     3481 attagtatca actgcattag atgaaactat agacttactt cctacattat ctgaaggatt
     3541 acctataatt gcaactatta tagatggtgt aagtttaggt gcagcaatca aagagctaag
     3601 tgaaacgagt gacccattat taagacaaga aatagaagct aagataggta taatggcagt
     3661 aaatttaaca acagctacaa ctgcaatcat tacttcatct ttgggggtag ctagtggatt
     3721 tagtatactt ttagttcctt tagcaggaat ttcagcaggt ataccaagct tagtaaacaa
     3781 tgaacttgta cttcgagata aggcaacaaa ggttgtagat tattttaaac atgtttcatt
     3841 agttgaaact gaaggagtat ttactttatt agatgataaa ataatgatgc cacaagatga
     3901 tttagtgata tcagaaatag attttaataa taattcaata gttttaggta aatgtgaaat
     3961 ctggagaatg gaaggtggtt caggtcatac tgtaactgat gatatagatc acttcttttc
     4021 agcaccatca ataacatata gagagccaca cttatctata tatgacgtat tggaagtaca
     4081 aaaagaagaa cttgatttgt caaaagattt aatggtatta cctaatgctc caaatagagt
     4141 atttgcttgg gaaacaggat ggacaccagg tttaagaagc ttagaaaatg atggcacaaa
     4201 actgttagac cgtataagag ataactatga aggtgagttt tattggagat attttgcttt
     4261 tatagctgat gctttaataa caacattaaa accaagatat gaagatacta atataagaat
     4321 aaatttagat agtaatacta gaagttttat agttccaata ataactacag aatatataag
     4381 agaaaaatta tcatattctt tctatggttc aggaggaact tatgcattgt ctctttctca
     4441 atataatatg ggtataaata tagaattaag tgaaagtgat gtttggatta tagatgttga
     4501 taatgttgtg agagatgtaa ctatagaatc tgataaaatt aaaaaaggtg atttaataga
     4561 aggtatttta tctacactaa gtattgaaga gaataaaatt atcttaaata gccatgagat
     4621 taatttttct ggtgaggtaa atggaagtaa tggatttgtt tctttaacat tttcaatttt
     4681 agaaggaata aatgcaatta tagaagttga tttattatct aaatcatata aattacttat
     4741 ttctggcgaa ttaaaaatat tgatgttaaa ttcaaatcat attcaacaga aaatagatta
     4801 tataggattc aatagcgaat tacagaaaaa tataccatat agctttgtag atagtgaagg
     4861 aaaagagaat ggttttatta atggttcaac aaaagaaggt ttatttgtat ctgaattacc
     4921 tgatgtagtt cttataagta aggtttatat ggatgatagt aagccttcat ttggatatta
     4981 tagtaataat ttgaaagatg tcaaagttat aactaaagat aatgttaata tattaacagg
     5041 ttattatctt aaggatgata taaaaatctc tctttctttg actctacaag atgaaaaaac
     5101 tataaagtta aatagtgtgc atttagatga aagtggagta gctgagattt tgaagttcat
     5161 gaatagaaaa ggtaatacaa atacttcaga ttctttaatg agctttttag aaagtatgaa
     5221 tataaaaagt attttcgtta atttcttaca atctaatatt aagtttatat tagatgctaa
     5281 ttttataata agtggtacta cttctattgg ccaatttgag tttatttgtg atgaaaatga
     5341 taatatacaa ccatatttca ttaagtttaa tacactagaa actaattata ctttatatgt
     5401 aggaaataga caaaatatga tagtggaacc aaattatgat ttagatgatt ctggagatat
     5461 atcttcaact gttatcaatt tctctcaaaa gtatctttat ggaatagaca gttgtgttaa
     5521 taaagttgta atttcaccaa atatttatac agatgaaata aatataacgc ctgtatatga
     5581 aacaaataat acttatccag aagttattgt attagatgca aattatataa atgaaaaaat
     5641 aaatgttaat atcaatgatc tatctatacg atatgtatgg agtaatgatg gtaatgattt
     5701 tattcttatg tcaactagtg aagaaaataa ggtgtcacaa gttaaaataa gattcgttaa
     5761 tgtttttaaa gataagactt tggcaaataa gctatctttt aactttagtg ataaacaaga
     5821 tgtacctgta agtgaaataa tcttatcatt tacaccttca tattatgagg atggattgat
     5881 tggctatgat ttgggtctag tttctttata taatgagaaa ttttatatta ataactttgg
     5941 aatgatggta tctggattaa tatatattaa tgattcatta tattatttta aaccaccagt
     6001 aaataatttg ataactggat ttgtgactgt aggcgatgat aaatactact ttaatccaat
     6061 taatggtgga gctgcttcaa ttggagagac aataattgat gacaaaaatt attatttcaa
     6121 ccaaagtgga gtgttacaaa caggtgtatt tagtacagaa gatggattta aatattttgc
     6181 cccagctaat acacttgatg aaaacctaga aggagaagca attgatttta ctggaaaatt
     6241 aattattgac gaaaatattt attattttga tgataattat agaggagctg tagaatggaa
     6301 agaattagat ggtgaaatgc actattttag cccagaaaca ggtaaagctt ttaaaggtct
     6361 aaatcaaata ggtgattata aatactattt caattctgat ggagttatgc aaaaaggatt
     6421 tgttagtata aatgataata aacactattt tgatgattct ggtgttatga aagtaggtta
     6481 cactgaaata gatggcaagc atttctactt tgctgaaaac ggagaaatgc aaataggagt
     6541 atttaataca gaagatggat ttaaatattt tgctcatcat aatgaagatt taggaaatga
     6601 agaaggtgaa gaaatctcat attctggtat attaaatttc aataataaaa tttactattt
     6661 tgatgattca tttacagctg tagttggatg gaaagattta gaggatggtt caaagtatta
     6721 ttttgatgaa gatacagcag aagcatatat aggtttgtca ttaataaatg atggtcaata
     6781 ttattttaat gatgatggaa ttatgcaagt tggatttgtc actataaatg ataaagtctt
     6841 ctacttctct gactctggaa ttatagaatc tggagtacaa aacatagatg acaattattt
     6901 ctatatagat gataatggta tagttcaaat tggtgtattt gatacttcag atggatataa
     6961 atattttgca cctgctaata ctgtaaatga taatatttac ggacaagcag ttgaatatag
     7021 tggtttagtt agagttgggg aagatgtata ttattttgga gaaacatata caattgagac
     7081 tggatggata tatgatatgg aaaatgaaag tgataaatat tatttcaatc cagaaactaa
     7141 aaaagcatgc aaaggtatta atttaattga tgatataaaa tattattttg atgagaaggg
     7201 cataatgaga acgggtctta tatcatttga aaataataat tattacttta atgagaatgg
     7261 tgaaatgcaa tttggttata taaatataga agataagatg ttctattttg gtgaagatgg
     7321 tgtcatgcag attggagtat ttaatacacc agatggattt aaatactttg cacatcaaaa
     7381 tactttggat gagaattttg agggagaatc aataaactat actggttggt tagatttaga
     7441 tgaaaagaga tattatttta cagatgaata tattgcagca actggttcag ttattattga
     7501 tggtgaggag tattattttg atcctgatac agctcaatta gtgattagtg aatagataaa
     7561 aatatgttaa atatatcctc ttatacttaa atatataaaa ataaacaaaa tgatacacta
     7621 cataaagtgt tctatctaat atgaagattt accaataaaa aggtggacta tgatgaatgc
     7681 acagtagttc acctttttat atttctaatg gtaacaaaat atttttttat ataaacctag
     7741 gaggcgttat gaatatgaca atatcttttt tatcagagca tatatttata aagttagtaa
     7801 ttttaactat atcatttgat acattattag gatgtttaag tgcaataaaa agtcgtaaat
     7861 ttaattctag ttttggaata gatggaggaa tcagaaaagt agcaatgata gcatgtatat
     7921 tttttttatc agtagttgac attcttacaa agtttaactt tttatttatg ttaccacaag
     7981 attgtatcaa ttttttaaga ctaaaacatc ttggaatatc tgaatttttc tctattttat
     8041 ttattttata tgaaagtgta agtatattaa aaaatatgtg cttatgtgga ttaccagtac
     8101 ctaagagatt aaaggaaaaa atagcaattt tactagatgc aatgacagat gaaatgaatg
     8161 ctaaggatga aaagtaagta atggtagata taataaagat attaacaaat aaaaagtgtt
     8221 atccaaataa gaatagctga aagttatcat aattcatgaa actaataatg aaaacgaggg
     8281 agcagatgcc aagagacaca caagtattaa atacatataa tttcgaagca agtgttcatt
     8341 actatataga tgacaaggta gtatatcaaa cattggttca caaagatggt gcatggtcag
     8401 ttggtaaaat ctattaagct acattagtta cagatatcac aaactataat agttaaacat
     8461 agaaatatgt gtaaattgtg atggaaatta ttcaaaaaca caaaaatacg tgatgaagga
     8521 caaaatgata tagaaaataa gtatcaaacc ttaataaatg atttaattga tagtttaaaa
     8581 gttataggaa aaatatataa agaaataaaa acattaaaaa aatataagat atgtttacaa
     8641 attactatca gacaatctcc ttatctaata gaagagtcaa ttaactaatt gagtatcttt
     8701 aaattgaaat gttaggaagt gatttaaata tgaaaactta aattataaaa aatcaatatt
     8761 aatttatttt taaaaaatag aaaggagtgt ataagattta ttttcaaagt ttaaaaacaa
     8821 gaaaatcaat ttaaatttca gaaggaataa atgtggttat agaagtggat ttattatcaa
     8881 aaataataat actaggaggt ttttatgtct ttaatatcta aagaagagtt aataaaactc
     8941 gcatatagca ttagaccaag agaaaatgag tataaaacta tactaactaa tttagacgaa
     9001 tataataagt taactacaaa caataatgaa aataaatatt tacaattaaa aaaactaaat
     9061 gaatcaattg atgtttttat gaataaatat aaaacttcaa gcagaaatag agcactctct
     9121 aatctaaaaa aagatatatt aaaagaagta attcttatta aaaattccaa tacaagccct
     9181 gtagaaaaaa atttacattt tgtatggata ggtggagaag tcagtgatat tgctcttgaa
     9241 tacataaaac aatgggctga tattaatgca gaatataata ttaaactgtg gtatgatagt
     9301 gaagcattct tagtaaatac actaaaaaag gctatagttg aatcttctac cactgaagca
     9361 ttacagctac tagaggaaga gattcaaaat cctcaatttg ataatatgaa attttacaaa
     9421 aaaaggatgg aatttatata tgatagacaa aaaaggttta taaattatta taaatctcaa
     9481 atcaataaac ctacagtacc tacaatagat gatattataa agtctcatct agtatctgaa
     9541 tataatagag atgaaactgt attagaatca tatagaacaa attctttgag aaaaataaat
     9601 agtaatcatg ggatagatat cagggctaat agtttgttta cagaacaaga gttattaaat
     9661 atttatagtc aggagttgtt aaatcgtgga aatttagctg cagcatctga catagtaaga
     9721 ttattagccc taaaaaattt tggcggagta tatttagatg ttgatatgct tccaggtatt
     9781 cactctgatt tatttaaaac aatatctaga cctagctcta ttggactaga ccgttgggaa
     9841 atgataaaat tagaggctat tatgaagtat aaaaaatata taaataatta tacatcagaa
     9901 aactttgata aacttgatca acaattaaaa gataatttta aactcattat agaaagtaaa
     9961 agtgaaaaat ctgagatatt ttctaaatta gaaaatttaa atgtatctga tcttgaaatt
    10021 aaaatagctt tcgctttagg cagtgttata aatcaagcct tgatatcaaa acaaggttca
    10081 tatcttacta acctagtaat agaacaagta aaaaatagat atcaattttt aaaccaacac
    10141 cttaacccag ccatagagtc tgataataac ttcacagata ctactaaaat ttttcatgat
    10201 tcattattta attcagctac cgcagaaaac tctatgtttt taacaaaaat agcaccatac
    10261 ttacaagtag gttttatgcc agaagctcgc tccacaataa gtttaagtgg tccaggagct
    10321 tatgcgtcag cttactatga tttcataaat ttacaagaaa atactataga aaaaacttta
    10381 aaagcatcag atttaataga atttaaattc ccagaaaata atctatctca attgacagaa
    10441 caagaaataa atagtctatg gagctttgat caagcaagtg caaaatatca atttgagaaa
    10501 tatgtaagag attatactgg tggatctctt tctgaagaca atggggtaga ctttaataaa
    10561 aatactgccc tcgacaaaaa ctatttatta aataataaaa ttccatcaaa caatgtagaa
    10621 gaagctggaa gtaaaaatta tgttcattat atcatacagt tacaaggaga tgatataagt
    10681 tatgaagcaa catgcaattt attttctaaa aatcctaaaa atagtattat tatacaacga
    10741 aatatgaatg aaagtgcaaa aagctacttt ttaagtgatg atggagaatc tattttagaa
    10801 ttaaataaat ataggatacc tgaaagatta aaaaataagg aaaaagtaaa agtaaccttt
    10861 attggacatg gtaaagatga attcaacaca agcgaatttg ctagattaag tgtagattca
    10921 ctttccaatg agataagttc atttttagat accataaaat tagatatatc acctaaaaat
    10981 gtagaagtaa acttacttgg atgtaatatg tttagttatg attttaatgt tgaagaaact
    11041 tatcctggga agttgctatt aagtattatg gacaaaatta cttccacttt acctgatgta
    11101 aataaaaatt ctattactat aggagcaaat caatatgaag taagaattaa tagtgaggga
    11161 agaaaagaac ttctggctca ctcaggtaaa tggataaata aagaagaagc tattatgagc
    11221 gatttatcta gtaaagaata catttttttt gattctatag ataataagct aaaagcaaag
    11281 tccaagaata ttccaggatt agcatcaata tcagaagata taaaaacatt attacttgat
    11341 gcaagtgtta gtcctgatac aaaatttatt ttaaataatc ttaagcttaa tattgaatct
    11401 tctattggtg attacattta ttatgaaaaa ttagagcctg ttaaaaatat aattcacaat
    11461 tctatagatg atttaataga tgagttcaat ctacttgaaa atgtatctga tgaattatat
    11521 gaattaaaaa aattaaataa tctagatgag aagtatttaa tatcttttga agatatctca
    11581 aaaaataatt caacttactc tgtaagattt attaacaaaa gtaatggtga gtcagtttat
    11641 gtagaaacag aaaaagaaat tttttcaaaa tatagcgaac atattacaaa agaaataagt
    11701 actataaaga atagtataat tacagatgtt aatggtaatt tattggataa tatacagtta
    11761 gatcatactt ctcaagttaa tacattaaac gcagcattct ttattcaatc attaatagat
    11821 tatagtagca ataaagatgt actgaatgat ttaagtacct cagttaaggt tcaactttat
    11881 gctcaactat ttagtacagg tttaaatact atatatgact ctatccaatt agtaaattta
    11941 atatcaaatg cagtaaatga tactataaat gtactaccta caataacaga ggggatacct
    12001 attgtatcta ctatattaga cggaataaac ttaggtgcag caattaagga attactagac
    12061 gaacatgacc cattactaaa aaaagaatta gaagctaagg tgggtgtttt agcaataaat
    12121 atgtcattat ctatagctgc aactgtagct tcaattgttg gaataggtgc tgaagttact
    12181 attttcttat tacctatagc tggtatatct gcaggaatac cttcattagt taataatgaa
    12241 ttaatattgc atgataaggc aacttcagtg gtaaactatt ttaatcattt gtctgaatct
    12301 aaaaaatatg gccctcttaa aacagaagat gataaaattt tagttcctat tgatgattta
    12361 gtaatatcag aaatagattt taataataat tcgataaaac taggaacatg taatatatta
    12421 gcaatggagg ggggatcagg acacacagtg actggtaata tagatcactt tttctcatct
    12481 ccatctataa gttctcatat tccttcatta tcaatttatt ctgcaatagg tatagaaaca
    12541 gaaaatctag atttttcaaa aaaaataatg atgttaccta atgctccttc aagagtgttt
    12601 tggtgggaaa ctggagcagt tccaggttta agatcattgg aaaatgacgg aactagatta
    12661 cttgattcaa taagagattt atacccaggt aaattttact ggagattcta tgcttttttc
    12721 gattatgcaa taactacatt aaaaccagtt tatgaagaca ctaatattaa aattaaacta
    12781 gataaagata ctagaaactt cataatgcca actataacta ctaacgaaat tagaaacaaa
    12841 ttatcttatt catttgatgg agcaggagga acttactctt tattattatc ttcatatcca
    12901 atatcaacga atataaattt atctaaagat gatttatgga tatttaatat tgataatgaa
    12961 gtaagagaaa tatctataga aaatggtact attaaaaaag gaaagttaat aaaagatgtt
    13021 ttaagtaaaa ttgatataaa taaaaataaa cttattatag gcaatcaaac aatagatttt
    13081 tcaggcgata tagataataa agatagatat atattcttga cttgtgagtt agatgataaa
    13141 attagtttaa taatagaaat aaatcttgtt gcaaaatctt atagtttgtt attgtctggg
    13201 gataaaaatt atttgatatc caatttatct aatactattg agaaaatcaa tactttaggc
    13261 ctagatagta aaaatatagc gtacaattac actgatgaat ctaataataa atattttgga
    13321 gctatatcta aaacaagtca aaaaagcata atacattata aaaaagacag taaaaatata
    13381 ttagaatttt ataatgacag tacattagaa tttaacagta aagattttat tgctgaagat
    13441 ataaatgtat ttatgaaaga tgatattaat actataacag gaaaatacta tgttgataat
    13501 aatactgata aaagtataga tttctctatt tctttagtta gtaaaaatca agtaaaagta
    13561 aatggattat atttaaatga atccgtatac tcatcttacc ttgattttgt gaaaaattca
    13621 gatggacacc ataatacttc taattttatg aatttatttt tggacaatat aagtttctgg
    13681 aaattgtttg ggtttgaaaa tataaatttt gtaatcgata aatactttac ccttgttggt
    13741 aaaactaatc ttggatatgt agaatttatt tgtgacaata ataaaaatat agatatatat
    13801 tttggtgaat ggaaaacatc gtcatctaaa agcactatat ttagcggaaa tggtagaaat
    13861 gttgtagtag agcctatata taatcctgat acgggtgaag atatatctac ttcactagat
    13921 ttttcctatg aacctctcta tggaatagat agatatatca ataaagtatt gatagcacct
    13981 gatttatata caagtttaat aaatattaat accaattatt attcaaatga gtactaccct
    14041 gagattatag ttcttaaccc aaatacattc cacaaaaaag taaatataaa tttagatagt
    14101 tcttcttttg agtataaatg gtctacagaa ggaagtgact ttattttagt tagatactta
    14161 gaagaaagta ataaaaaaat attacaaaaa ataagaatca aaggtatctt atctaatact
    14221 caatcattta ataaaatgag tatagatttt aaagatatta aaaaactatc attaggatat
    14281 ataatgagta attttaaatc atttaattct gaaaatgaat tagatagaga tcatttagga
    14341 tttaaaataa tagataataa aacttattac tatgatgaag atagtaaatt agttaaagga
    14401 ttaatcaata taaataattc attattctat tttgatccta tagaatttaa cttagtaact
    14461 ggatggcaaa ctatcaatgg taaaaaatat tattttgata taaatactgg agcagcttta
    14521 actagttata aaattattaa tggtaaacac ttttatttta ataatgatgg tgtgatgcag
    14581 ttgggagtat ttaaaggacc tgatggattt gaatattttg cacctgccaa tactcaaaat
    14641 aataacatag aaggtcaggc tatagtttat caaagtaaat tcttaacttt gaatggcaaa
    14701 aaatattatt ttgataatga ctcaaaagca gtcactggat ggagaattat taacaatgag
    14761 aaatattact ttaatcctaa taatgctatt gctgcagtcg gattgcaagt aattgacaat
    14821 aataagtatt atttcaatcc tgacactgct atcatctcaa aaggttggca gactgttaat
    14881 ggtagtagat actactttga tactgatacc gctattgcct ttaatggtta taaaactatt
    14941 gatggtaaac acttttattt tgatagtgat tgtgtagtga aaataggtgt gtttagtacc
    15001 tctaatggat ttgaatattt tgcacctgct aatacttata ataataacat agaaggtcag
    15061 gctatagttt atcaaagtaa attcttaact ttgaatggta aaaaatatta ctttgataat
    15121 aactcaaaag cagttaccgg atggcaaact attgatagta aaaaatatta ctttaatact
    15181 aacactgctg aagcagctac tggatggcaa actattgatg gtaaaaaata ttactttaat
    15241 actaacactg ctgaagcagc tactggatgg caaactattg atggtaaaaa atattacttt
    15301 aatactaaca ctgctatagc ttcaactggt tatacaatta ttaatggtaa acatttttat
    15361 tttaatactg atggtattat gcagatagga gtgtttaaag gacctaatgg atttgaatat
    15421 tttgcacctg ctaatacgga tgctaacaac atagaaggtc aagctatact ttaccaaaat
    15481 gaattcttaa ctttgaatgg taaaaaatat tactttggta gtgactcaaa agcagttact
    15541 ggatggagaa ttattaacaa taagaaatat tactttaatc ctaataatgc tattgctgca
    15601 attcatctat gcactataaa taatgacaag tattacttta gttatgatgg aattcttcaa
    15661 aatggatata ttactattga aagaaataat ttctattttg atgctaataa tgaatctaaa
    15721 atggtaacag gagtatttaa aggacctaat ggatttgagt attttgcacc tgctaatact
    15781 cacaataata acatagaagg tcaggctata gtttaccaga acaaattctt aactttgaat
    15841 ggcaaaaaat attattttga taatgactca aaagcagtta ctggatggca aaccattgat
    15901 ggtaaaaaat attactttaa tcttaacact gctgaagcag ctactggatg gcaaactatt
    15961 gatggtaaaa aatattactt taatcttaac actgctgaag cagctactgg atggcaaact
    16021 attgatggta aaaaatatta ctttaatact aacactttca tagcctcaac tggttataca
    16081 agtattaatg gtaaacattt ttattttaat actgatggta ttatgcagat aggagtgttt
    16141 aaaggaccta atggatttga atactttgca cctgctaata ctcataataa taacatagaa
    16201 ggtcaagcta tactttacca aaataaattc ttaactttga atggtaaaaa atattacttt
    16261 ggtagtgact caaaagcagt taccggattg cgaactattg atggtaaaaa atattacttt
    16321 aatactaaca ctgctgttgc agttactgga tggcaaacta ttaatggtaa aaaatactac
    16381 tttaatacta acacttctat agcttcaact ggttatacaa ttattagtgg taaacatttt
    16441 tattttaata ctgatggtat tatgcagata ggagtgttta aaggacctga tggatttgaa
    16501 tactttgcac ctgctaatac agatgctaac aatatagaag gtcaagctat acgttatcaa
    16561 aatagattcc tatatttaca tgacaatata tattattttg gtaataattc aaaagcggct
    16621 actggttggg taactattga tggtaataga tattacttcg agcctaatac agctatgggt
    16681 gcgaatggtt ataaaactat tgataataaa aatttttact ttagaaatgg tttacctcag
    16741 ataggggtgt ttaaagggtc taatggattt gaatactttg cacctgctaa tacggatgct
    16801 aacaatatag aaggtcaagc tatacgttat caaaatagat tcctacattt acttggaaaa
    16861 atatattact ttggtaataa ttcaaaagca gttactggat ggcaaactat taatggtaaa
    16921 gtatattact ttatgcctga tactgctatg gctgcagctg gtggactttt tgagattgat
    16981 ggtgttatat atttctttgg tgttgatgga gtaaaagccc ctgggatata tggctaaaat
    17041 atatgtttga taaaaaatta ttcctgtgct actaagaaat tatttttata taataaatat
    17101 tgagatttaa ttaaaagtca tgtgttattg taatacatga cttttagtta aaatttttct
    17161 atcatttaat aatctattat tcttgactat tttataataa aattcatata tggaaatatt
    17221 aatactaaat aattaatagt tgataaaaaa tagataatat gctaaaagca aaaactaatt
    17281 tagagccttg taactgttta tttgcaatta taaaaacatc tttaaacata ttgactataa
    17341 tataaaatat taactataat acaaaacaat attaattaat tttctctaca gctatccctg
    17401 gtatagttat atttccaccc atagttgatt cataagttat agttccagca cttatacctc
    17461 ttatagttat gtaatcatcc tctaatactc ttgaaggagt tattgatttt ttataactac
    17521 atagtacaac cttatcataa tttccagaca cagctaatct tatttgcacc tcatcaccat
    17581 cttcaataac ttgaataacc ttaccttcaa attttacctt tttgtactta taatcatcag
    17641 gtgttctagc taattggtca taagtaatac cagtatcata tcctttcttc tcctcttctt
    17701 cttttttgcg ttgttcttca gcctttttag cttcttcagc ttttttagct tcttcagctt
    17761 tacgttgatt ttcagcttca atagctttct tttcgtcgtc tttcattttg aaccatggtt
    17821 cagcatcaga caatttgcta tttaaagttt ctatttctgt tttttgagaa tctataactg
    17881 atgtttggtt agaaatgacc tcctcatggt cttcagaaca agctggtgag gatatattgc
    17941 caaaaaacac gcctaaaata aatgccagta gagcaatacc ctttgtgctc ttaaagaatt
    18001 taattatttt cttagagctt ccttttcctt cattactaaa ttcgttacca tcattttttt
    18061 tagaaaacat aatacaatct cccttttatt agatatattt tattttaaaa gttaaaaact
    18121 taaaataact agttgactaa aaattatata ttcatatttt cctaaaatat atttccccta
    18181 agcataattt tacaaaataa agtaagaaga ttcaatatat tttgaaaaaa taagataaaa
    18241 atgtcagatt ataatataat tttcttgaag gtgaatttta tatattaaat taaacataaa
    18301 gtattttatt tgtataaaac tatattatga tgtaattaag tgactcagtg atttagaggt
    18361 ggtaaaatat atatatatga aagagcataa ataaattgat ataataaaaa attaaatatt
    18421 aaatattaac tataataaaa aaatatctgg taataggtat aatataaaaa gaatatacca
    18481 gaaagaaggt gaagtatggg agaactacaa aaaattccag gggtagggaa agcaacagaa
    18541 aaatctttaa tcatgttagg atatacaact attaaatcac ttaaagatgc aaacccagca
    18601 caaatgtatg aaaaggaatg tttaatacgt ggacagcaca tagacagatg tcagttatat
    18661 gtatacagat gtgcagtata ttttgcatca actgaaaatc cagaaccaga aaaattaaag
    18721 tggtggtatt ggaaagatta aaaaatcaaa aaagttagac ataaaaaaga gttgaatatc
    18781 cgttttaaat aataaaaact aatactcaac tcttttttag tcgtaaattt tatgtaaatc
    18841 tatttgtatg gctaaatata cccatcaata aatgtataaa tataccaatc aacaaatgac
    18901 taaacatact acctaacaaa ggattaaata aattacttaa caaagcattc taaaacaatt
    18961 aattagaaac tgtttcttca agccattttc tacattgctc aatatttacc atagggtcaa
    19021 aaccttggtc ttttccctta tttaaaagca tatcacaagg gaaagtatca cattcaccac
    19081 aatgattaag ttccttactt tcacagcaag ttttaacctt acattctcca ccccaaaatg
    19141 gtttttccat agttgggcaa ccagtgcaat ttacttgttc tttttttgta caactgtcac
    19201 aacagacacc acatcttgat tcaaacatgt tcaaacatcc tttccattat ttaaaaatat
    19261 tataaaatga ttttagatga atacaaaagt gcaaatagtt gattttaggt catctaaatg
    19321 ttaaacataa aattattagt actggtaaat ttataaaaac tattccaaaa atctttttga
    19381 atatttcaaa acacaataaa ttacttcaaa tccctttgaa tatattttac agcattatag
    19441 attgctttaa atctctttga atattctaca agactataga ttagtttaaa tccctttgaa
    19501 tatattttac aacattatag attgttttaa atctctttga atattctaca agactataga
    19561 ttagtttaaa tccctttgaa tatttcaaaa caccatagat tactttaaat ccctttgaat
    19621 attctataac actataaatt acttttaact tttatataac cataaagttt ttatctggca
    19681 ctttaaatgg agttttatat caaatttgcc ttccatcgaa taaccaaact taatttaatt
    19741 acacattcca agagtcaaat attcattcaa tatatttgat aattctatgt tatagctata
    19801 attaaatata gattattaaa gttaaatatt tatttaatat attgaatgat tttttaccta
    19861 tatttaatta agtttcatta tataatataa aaattgtttt tgca
//
LOCUS       DAEMGG010000070        19644 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEMGG010000070 DAEMGG010000000
VERSION     DAEMGG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.070"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(8..667)
                     /locus_tag="KPK24_002312"
     CDS             complement(8..667)
                     /locus_tag="KPK24_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2299982.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKI
                     R"
     gene            complement(1198..1575)
                     /locus_tag="KPK24_002313"
     CDS             complement(1198..1575)
                     /locus_tag="KPK24_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2299983.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            complement(1762..2733)
                     /locus_tag="KPK24_002314"
     CDS             complement(1762..2733)
                     /locus_tag="KPK24_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2299984.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFRNALWMYKFTGLFIKDKTK"
     gene            complement(2767..2964)
                     /locus_tag="KPK24_002315"
     CDS             complement(2767..2964)
                     /locus_tag="KPK24_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG2299985.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            complement(3032..4243)
                     /locus_tag="KPK24_002316"
     CDS             complement(3032..4243)
                     /locus_tag="KPK24_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG2299986.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            4524..5126
                     /locus_tag="KPK24_002317"
     CDS             4524..5126
                     /locus_tag="KPK24_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2299987.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKSVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
     gene            complement(5382..6353)
                     /locus_tag="KPK24_002318"
     CDS             complement(5382..6353)
                     /locus_tag="KPK24_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG2299988.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(6379..7860)
                     /locus_tag="KPK24_002319"
     CDS             complement(6379..7860)
                     /locus_tag="KPK24_002319"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG2299989.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            complement(7879..8027)
                     /locus_tag="KPK24_002320"
                     /pseudo
     CDS             complement(7879..8027)
                     /locus_tag="KPK24_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            complement(8196..9182)
                     /locus_tag="KPK24_002321"
     CDS             complement(8196..9182)
                     /locus_tag="KPK24_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2299990.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKKLKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     EMSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            complement(9566..11320)
                     /locus_tag="KPK24_002322"
     CDS             complement(9566..11320)
                     /locus_tag="KPK24_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG2299991.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            complement(11538..12428)
                     /locus_tag="KPK24_002323"
     CDS             complement(11538..12428)
                     /locus_tag="KPK24_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2299992.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
     gene            12577..13437
                     /locus_tag="KPK24_002324"
     CDS             12577..13437
                     /locus_tag="KPK24_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2299993.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            13893..14459
                     /locus_tag="KPK24_002325"
     CDS             13893..14459
                     /locus_tag="KPK24_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2299994.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            14499..15074
                     /locus_tag="KPK24_002326"
     CDS             14499..15074
                     /locus_tag="KPK24_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2299995.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGDGDDEQINVDLSKIPQNIA
                     KISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAELY
                     RHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(15131..16048)
                     /locus_tag="KPK24_002327"
     CDS             complement(15131..16048)
                     /locus_tag="KPK24_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2299996.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            16178..17809
                     /locus_tag="KPK24_002328"
     CDS             16178..17809
                     /locus_tag="KPK24_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2299997.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            17812..18759
                     /locus_tag="KPK24_002329"
     CDS             17812..18759
                     /locus_tag="KPK24_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG2299998.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(19044..19307)
                     /locus_tag="KPK24_002330"
     CDS             complement(19044..19307)
                     /locus_tag="KPK24_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG2299999.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(19406..>19644)
                     /locus_tag="KPK24_002331"
     CDS             complement(19406..>19644)
                     /locus_tag="KPK24_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300000.1"
                     /translation="DEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSGYYA
                     LLENKEERVVWDGEVVANNIFNNLWIVINKVKTG"
BASE COUNT         6777 a         2933 c         2378 g         7556 t
ORIGIN      
        1 ttaaaattta tcttattttc ctaattttaa tcttaagtat tatatgtatt aatacaaaga
       61 ttgaaatgtt agataatatt aatagtaaaa ctattactaa aaaagcagtt cttctttcag
      121 gagagatttt tacaagatta ataatataat cataaagtac aaaaaatgac aataaatttg
      181 ctatcatagg aatacatatg tatatctctt ttttacttat tttaaaattt ttaaaagaag
      241 ccttataaac ataaagtaat atacctaaca ttatctttcc caagaatatt gcttcatatc
      301 tatataaact gccactaagt aaaacattca tattatccaa attgcttact gttgctacta
      361 ataacatact taatccattt atacctatta atattaacca ataaaaaaat gagattacca
      421 gacatttata tatattaacc ttatagttag acttatagaa aacgaacatt acaaatatac
      481 atatcaagat tctactatta gctcctatat ttatatataa agaagaaata atgagaaaaa
      541 cagaaaagag aaaatacata tttagttttt ttaagtcggt ctttgactga ctagtataat
      601 caaaaacttt tttacatgac catatttcta taatagaaga aacaattgtt ataaatgcat
      661 ctaacataat aatatcctca aaaatgatta aattatattt tttataaaat atatcataaa
      721 aaatacatac taattatagc ataaaaaaca cacttaaatt taatataatt atttaatatt
      781 tttaacaact ttctatagta cattttaaaa tatttacaga taagttacca ataataaagt
      841 ttaaaaatat aaaaatcatt tcttactttt agtttaccta taataacaaa agacaggttt
      901 atattgtaat aattaaaaat agtaaaatat attttgatat ggttaaatag aaataatact
      961 ttagattaat gaatattgac aagttataaa attattttaa attttaaaaa tcatttctta
     1021 tttaaataag tggtatcatt taacttgata ctccctattc caattatttt aggcatttta
     1081 ctaattagag aacctattta atattacttt atgaatagtg agtagctgat taaataatac
     1141 catttatggt tttcagccat agattaatta aattcttaga gttctatcct gatataatta
     1201 ctcttcttct aaatttttaa aatgactttc aaataaatcc aacatatctt tatcaacaac
     1261 ttcatcatca tgtagtgctg aaattaaact ctgtaaagaa ttgtcgtgta tattgcttaa
     1321 aaaatctttt gtctcaacac ttaaatactc cttatgtcca acaactattg tataatgagt
     1381 gtatctatct attttttctg cacttaaaaa atgtttttta acaagtcttg ataaaagtgt
     1441 caatgtagtt gtttgtttcc aaccatattt tcgttccata gcttcaatag tttcttttga
     1501 tgttactgta tcattttttt cccaaataaa cttcataact tttaattctg cttgtggtat
     1561 ttttgaaatt gtcataacct tacgcccctc tcaaactttt attaaattaa tactatcgca
     1621 atatagtttt taagtcaaag attttgtcga taataatatt ttccaatttt taccataggg
     1681 ttatttttta tataattcta tgataaatta cataaaaaat atttatttta catcataaaa
     1741 ttagaaatgt aaatagtatg attatttagt cttatcttta ataaacaatc cagtaaattt
     1801 atacatccaa agtgcattcc taaacaacaa taaacttgtt gaaaaaaata ctgttctaat
     1861 acctaaatgt gctgatattt gtccccctaa aaatgctcct gatgaacagc ctatatactg
     1921 ggcagatgca ttatatccaa atatttttcc cacatattct tctggtgcaa tttttttaag
     1981 caagctgtta acagaaggat taagccctgc aactgacaac cctaataaaa atctcaacaa
     2041 catcagttgc catgcagttt ttacaaaagc ttgtggtata aatataatcc cagcccataa
     2101 aagtgaaatt aacaatactt tttgaggacc tattttatcc gacatttttc caagtttttg
     2161 agctgctaat aaatttgcta atcctgatgc agaaaatgtc aatccagcta ttaaagctac
     2221 atttgatgca aaatttgtaa gttgtgatat atatatggtt acaattggtt ctatagagta
     2281 taaagctagt tgagtaatga atgtggttac aaaaatagat gcaattaaaa atttatttgg
     2341 caatatattt aattgttctt taggtataga aattgattct tttatttctt taggtttaaa
     2401 attatctact acaaataaaa tagagatgat aaatgataca aacaacaatc ctcctgttat
     2461 aataaatgta cttttaagac ctaagattga ttctatatat cctcctactg ttggaccaat
     2521 taatgagcca gtagttgatg cagttgcaag agtacccaaa gcccatcctg aatgattttt
     2581 atctgtctgt gttgcaataa gagtagtaca tgcagtagca taacccgtaa aaactccttg
     2641 aagtattctt aatcctaata actgataaac attttgaaca aacgccatgg taaatataac
     2701 tattcccatc ccaatacttg cacgtaaaag cattgatttt cttccatatt tatcagcagc
     2761 tttccctcaa attggcgaaa agaatgctgc tactacaaag gtacatccaa aaattatacc
     2821 tgaatactgt tgtattaaac taacatcagt tacccccaac tgtttaatat ataatggaag
     2881 tataggtgct atttggctca tccctataga actaaaaaac atacctatcc aacatacaaa
     2941 taaatttctt ttccacaatt ccatatagaa ttacaccccc tgcattttaa attttttcaa
     3001 taatactaag tttattaatt tatgctattg tttactcaat atatccatct tttcttttat
     3061 aatattgttt aggaaattca taacttgaaa ataatagcgg tgataaagaa taggagtaac
     3121 ttccagcatt actaattgaa atcaaatctc ctatgtttgc ttttttcaat ttaacattat
     3181 ttgcaattac atctaatgat gtacagagat tcccaacaac atttacatac tcttcttctt
     3241 ttaaattatt taatactgaa agagaaaagg catgatttga tgtatataat ggctcttgac
     3301 cttctaattc tactccacaa gtaatatttt caattaaatt agaaataata ggtctcataa
     3361 atccattaag tccatttttt acaataagat attttttccc tcttgatact ttaatatcta
     3421 caattttaac ataataatta cctgatttac aaactatgaa acgtcctgat tcaataagaa
     3481 gtcttgattt tagtgtgttt gaattagtaa aagcaatctt ttgagttaat tttgaaagct
     3541 ctttaagatt aagtggattt tctctaagat tatcataagc tactccaagt ccactaccaa
     3601 aatttataaa ttcaatatta atatacttac ttttatttaa atttaaagct ataaaaaaac
     3661 atttttcata ataatgtcct aacttttcaa aatccaatac ctgagattta atatgtatat
     3721 gaatcccaac tacagataca taatcacaca tacttaacat cttaaaaaca ctgctcatat
     3781 cttctatatc tataccaaat ttgcttgatt tagcactatc atcatccata gtaaaatcag
     3841 gatttattct tattcctatt ttaactttct gattatgctt tttagatatc ttatttataa
     3901 gttctatttc tgtaatacta tctgcaataa ttatgcattt atctaaaaca gtttctatat
     3961 caatctctgt tttgcctggt gatgaataat aaatatcatc tttagaaact ccacattttg
     4021 atgaaatcat aacttcatta gttgaagaag catctgctcc aaaaccttgc tctgcaatgc
     4081 tttttacaat tttgttatat ggatttgttt ttatagaata taaaaaatca aaacctaaaa
     4141 tattattttt caattcctta catttttcaa ttatattagt ctcatcatat atataacatg
     4201 gtgcattttc ttctataaat ttatagtctt catttgagta catatttttt acctccaaga
     4261 ttttaaatat aatcataaat taagcaatta taatcctata agataatttt acaataaatt
     4321 accatagtta actacacaca tatatgtaaa aacataccta aaatattttt aattatagat
     4381 acattccaac ttttattgat aaaacaattt gtttcaattt tattacaaat atacaaagtg
     4441 tattgttgtt aacgtattct catagtatcc taaaactata ttaagcacta aaaactattt
     4501 taaaatactg tggaggtatc aatatggaag tatgcattct tatgggaagt cctaagaaaa
     4561 aaggtaatac tgctgctatt ttaaaacctt ttattgagga attagaaaat tataattcaa
     4621 atattgaagt aatatggtta tatgattgta aaatagagcc atgtattgct tgtaaaaagt
     4681 gtcaggataa cttatttgat tttggatgtt attgtaaaga tgatgttcag aaaattttca
     4741 agaaaattct tatgtgtgat ttaattgttt ttgctactcc aatttattct tggtactgca
     4801 cttctccaat gaaagcacta cttgacagat tggtgtatgg aatgaataaa tactatggta
     4861 atgagaaagg ccccgctcta tgggcaggaa aatcagtagc acttattaca acatgtggat
     4921 atagacctga aaagggtgct gatatttggg aaaatggtat gaagcgctac tgtaagcatt
     4981 cacagttaaa atatatagga atgcttgttg aacgtgattt aggtaaacct aattttatga
     5041 ataaagaaaa agaagaacat tctcgtcaat ttgcaaaaca aatttattat attttatcta
     5101 attcaattga attagataac tgctaaagac ataatactcc aagtaaagta ttgataatat
     5161 aaataaatct tacataattt gcttatttaa aataagatat atattcttct aaaaagtaac
     5221 aataattaaa atatttttta tcatttcaac aatgataaag aggcttatct ctattttgag
     5281 acagcctcct tattgctttt tatatgttaa ttattttcat agtaaacatc cttaaaaact
     5341 ctttacatgt tacttccttc aaaacctatt actcattcct actataaatt ttttataaat
     5401 tcagttattt cttcctttgt tgcatatgaa cttatcgcac ctttaatagt tgtactacga
     5461 gctgcataat aattagaaaa ttctaaatac tcatttaacg tttcttgttt catttctttt
     5521 atatcattca atgttatttg tttatacaat aaggtgtata aaaatgaacc tatataagaa
     5581 tctcctgctc cggttgtatc tattgcattt actttaaccc ctttacacat accttttact
     5641 tttttagtgt atgcatctac tccatctttt ccttttgtat ataatactag ttcaacattc
     5701 cccacaaaga gtttatgtaa tgatttttct atatcatttt ctcctgttat aaattctaat
     5761 tcttcatcag atatcttaac aatatgagca aaaggtaaaa attctgatat tgccttttta
     5821 caagattgct cactatccca taaaggtaat cttatattag ggtcaaagct tattatacta
     5881 ttactttcaa gtgcgtattc tattgctttt ttatgtgcta atttcattgg actatctatt
     5941 aaatctactg aacaaaaatg taggatatga caattattaa accactcttt tttaacttca
     6001 tcagcttcta aaagcatatc tgcacttgga tttctataaa atgagaaatc acgattacca
     6061 tcttctttta atgatacaaa agcaagtcct gtatttgctt tacttgttct caatacataa
     6121 tcagtattta catttacttc atttaataca tctattatat aatcaccaaa tgcatctttt
     6181 cctaacttag atataaaact cgattttcct ccaagctttg ctacaacagc actaacattt
     6241 gctggagctc ctcctgctac tcttctaaat tctgatacat tttttaattt accaccattt
     6301 tgattaggta taaagtctat taaagcttct cctatactaa ttactttttt catcttttct
     6361 accccttata atgtgttttt ataattcaat accttgcttt aatttaaatt tagtatactc
     6421 atactctact attttatcac tataaaccct tatttgatta gaattttctt tagcaaatat
     6481 tctactagta aatactactt ctccttcatt tataaaaatt tctaaaatac ttctatccgc
     6541 tagtactttg atattagtat tttcatcaat atttatatat tcttttctat ttgttccata
     6601 ttcaacaccg aaaacttttt taaagttgct tctgtctaaa gttacaatgt ttcctaattt
     6661 gttaaattct aattttacac cttcatcttg tgatgaaaat aattccagcc caaatttatt
     6721 gctgtcaata ttttttatat taatattaag ctcatataca ttttcatcat tttctataat
     6781 attaatatta ttctttatag tattttgccc acttgtttca tcatatctta atttaattaa
     6841 ttcatctact ggcatttgca atagcttatt attttttata tttaattttc ttggaacagt
     6901 tagacaatgt gcccatttgt ttttatctgt agggtataaa acttctccca ttccagccca
     6961 tgcaaatagt attatttgtg agtcctttat aaatgtttgt ggagcataga aatcaaatcc
     7021 tttttccagt tctttcattg tatctatttc aaatgttaaa ttatctaaat ccatattacc
     7081 aatagcatat actacattat atatattttg ataatcaaat cctttaggtt ctactccttg
     7141 tggtgaaaat attaatatat ctttttcatc tatattaata taatctggac attcccacat
     7201 gtaaccaaaa tcttcatcta tgttttttac atttaattct cctttaaaat tccaatctat
     7261 agagttttta gattcatata ctattataac tcctttttta tcacttgttt gtgctccaag
     7321 taacataaag tatttatcct ttcttttaaa tacttttggg tctcttacat gtcctgtata
     7381 tccttttggt atttcactta ctattggatt attttcatat ttagttattt taccatcctt
     7441 gttcattata gctaagtttt gatatgcata tctatcttct gcactatatt ttatattccc
     7501 tgtataatac atatgcaatt ttccatctac ctctatcgca ttacctgaat atgctccatg
     7561 agattcataa ctttcgattg gtattaatgc tacatcttct ctattccagt ttacaaaatc
     7621 atcagaactt acataagccc aatgcttcat tccatgagtt gcatcatatg gataccattg
     7681 ataaaataca tggtacttac cattataata tgctaatcca tttgggtcat taagtagtcc
     7741 atgttgtgga tgtatatgaa atagtggttt atatttatca tttaaagata tatttcttaa
     7801 tttatttaat tcttcttttg tcgattctaa tatggttcta tatttaggct ctttgtacat
     7861 atttttattc cttttctttt ataattttat atttgcttta ttattgctat ctaaaacaat
     7921 tcttagtctt attacacaat gtgctacatc ttttatattt tgtttattcc tatgtactcc
     7981 aataattact ttgatacttt gttacaatca gttatttttg cactcatttt gctattctat
     8041 cggtttaatt aaaccggttt cgaaaccggt ttaattaaag attagcataa atttatttca
     8101 gatgcaatat ttctttcaaa aaagttttcc taatttttag aattatagtt tttttagtaa
     8161 aaaaatacac attactaata tatgtaatgt gtatcttaat ttttatttat tgtactttca
     8221 ccttctatta attcaaattt aatctccttt aatagtggtt cttttctttc ttgtattaaa
     8281 tttactatcg tatttgctgc tactttacct gcatttttat tttcaaactt tattgttgta
     8341 agtttagggc ttataataga caatacatca tatcccccaa atccagctac agatatatct
     8401 tgtggtatac ttttacctct cttattgatt tctttcattg ttgctattgc tatattatca
     8461 gttgcacata ttattatatc tggattttca ctttctagca tttctccact tctttgtata
     8521 gacatttctt ggtcaaagtc agtataaaat acttttgcat catacccttt atcttttaac
     8581 ccattaagta ctccattttt tctatttaaa ccaactgata tatcactttc atcaacacct
     8641 aaatatacaa tgtttttgta acctttatta gcaatatatt gacccatttt atagcctgct
     8701 ccataatcat cattcactat gctattgata ccatcaactt tttgtccaac tacaactatc
     8761 ggtatatcta acttttcaat ttcattctta tgttctttag ttatttcggt accaattagt
     8821 attattccat caacttttag actagctaac tttgatatac tatctatttc tgaccttatt
     8881 tttctattag tattaataat tagtgatgta tattttaatt ttttgagttc ttcatctatt
     8941 gccatcataa ttttagattt tacaaaagaa tctaaacacg gtgttataat accaataaaa
     9001 tatgtttttt tagctctcaa actttgagca aatgcattcg gctcatatcc atattcatct
     9061 attattgtct ttattttctc tttagtagaa tcactgatat ctttattgtt tagatatctt
     9121 gatacagtac ttttagatac tcctgccaag ttagcaatat catttattgt tatttttttc
     9181 ataggttatc accttctaca tttgatatat tttattacat tatattatat atttaaaata
     9241 aaatatagtt atttgtccag tcaatacttt ttatatacta cttgtactat ccattccaca
     9301 tccaaataat tgagtcttta acattaaatt aattgtaatt ttcatttttt gttgttatat
     9361 ttaatttcaa ttttatctac tatgaattga taaatactct aatttattta taatataaaa
     9421 ataaggctat gatttttaca accatagcct tatcgataaa cacttaatta tcacttaaaa
     9481 aataattttt tatttaaaga atatatataa atattttaaa attctataat ttttaaaact
     9541 atataaactt tattttcata tgtttttatt ctccaattga tatattattt atgccattat
     9601 ttttaacttc aataattggt tgtccatttc ctaaaatata atctttagac caaccttctg
     9661 gcaatttatt tatcccattt ccaattaact tcaagtgtaa atttgatggt tcttttttca
     9721 gatttaattt aaatattcca tcaaaagtat ctttattatc gcttccaaat aaacttatat
     9781 tgccatttat atttgtagct gttacattta caattccatt gtttgctcta aattttattt
     9841 cagatgatat agtttcagct gttacacctg aaatcccact attagtattt atattgaaag
     9901 gatttgttac attgttatca acaatgttag caattccgtc attgacatca acagtagctc
     9961 ctcctttaat attataaagt tctactgaac cactttcaac atctacttta aaattagaat
    10021 acattttatc gggaactcca atttttatca catttaccat cctatcttta tttgcattta
    10081 tcatattgtc gccattcata tcttgcaaat cactatcttt cttatctatt tttttcttag
    10141 cacttacaac tagctttcca ttttttatat tattagaaga attttcttta tatacagaaa
    10201 aagtacaatt tttttcatta gttacactta cgtaatcaaa ctcaaattta ttggtattcg
    10261 tcttttcaac aaccacacct aaattatctg attctataag tatttcagaa acttcatttc
    10321 cttcaactga agttaagtgt tttaaaatat ttggagtcat atccatatta ttatatttaa
    10381 tcatatctaa acactgttta tatatattgg catcaatacc tccaactttt gttatacttt
    10441 tagctccttt taaaatacct ttattacttc catcagcaac taaaacgatt ggtttttctt
    10501 tggctaaagg agataatgtt agtgcatcta ctaattgata tccatttgtt atataaaaat
    10561 ctgtagctcc attataaaac ttttccatta cctttttatt ggtctcaaat ctatcatttc
    10621 ctccgattct aattgcttta gtttcattta ctaaatcctc atttatcgca gatttgccac
    10681 ctatagcgta aacatttaaa ccttcagtct taaatggaac tgatttccca tctgtaagta
    10741 ttatagctcc tttatttttt gcaactacag gtgctatact catagcatca gcctctccta
    10801 caaatccatt tgtaagcatt acctctttta cactacttac ttcatttatt tttttagcta
    10861 cattataact agtttcaatt ctattgttac catttattct ttccacttga ataccttttc
    10921 tttttatatc tttttctact gaattttcaa tagtattata accaccaatt atatatactt
    10981 tttttacaac atttaaactc ttattatcta gtctatttaa agtagctttt ggtatttcac
    11041 tttttttagt caaaagtatt ggtgcgttaa tagctccagc taatccactt gatgaaagcc
    11101 catcagataa atttttatct ccattaacta atattacagt atcataagtt tgtctatccg
    11161 ctataagtcc tgctgtttca tatctatcaa ttcctattat tgaatcttct ttaatctgtg
    11221 catttgcagt taaattatta aaaagaaaca tagtcaaaac taaaattaat atacttagtt
    11281 ttttactgaa ttttttgaat tttttattca taatatacac tattcctcct gtaaattaag
    11341 gtctttaatt tgcttataaa ttatcccctt atttacaaat aataacataa tattatagta
    11401 acgtatatat taccttaaaa ttatcagtaa ctttatatca atcatcttag cttatttata
    11461 taaatttttt tatattactt ttcaaataac agtcatacta aaacaatttc atcatataat
    11521 ttgaaaatta ttactattta cgcagctttt atcatcatct taccaccatc aaatatatat
    11581 tcaaccatat tattctcata taaataagtt atataagaac gtattgtact tccaaccaac
    11641 acatattgat tagcatcaag cttgacattg tacaaatcac acattttttc aataacttca
    11701 tcaatcataa ctggctcata acaaaccttt ttaatattat ctattatttc ttccatcttc
    11761 ttcttattaa tattaattaa actttttata tctgtagttt tgacagagtg acttggaaca
    11821 aagaaatcag cctctaacat tctcaatttt tctagtgtct taaactgtga atctatatcc
    11881 agtaggaaga aaaaatgata tttagtaatt atattctctg gtgtaagact atctgcaaca
    11941 aacaaaacat tatctggagt tctaacacca atcatttcaa aataatgacc tggaagagaa
    12001 atcgcttcaa gttctgtatc tacaattttg ccatttgaag gtataatata atcaacttca
    12061 ctttcctttg ctaataaaaa tttattcttc agtgctttac ttggatagcc accatataaa
    12121 aatgctgatt ctaatattgg attttctgta aaatatcctt caattttagt cgtagcaatc
    12181 ttgcattttg tttgattctt caaatatgca tttcctccta tatggtctgc atttgaatga
    12241 gtgtttatta ttaatttagg atttaaatta tgttcttcta ataatcttaa aacttgtcta
    12301 gctgtatcct tattatttcc actgtcaatt aaaattgcat ccttaccatc tacgtataca
    12361 cctactattg tagggagtgg aatataatat gtatttcctt ttatatgttc tagagttaat
    12421 ttagccattt tataacctcc ctttaaattt acttttaatt atagcattaa ttttctgaaa
    12481 attccaatca aattgttttt ataatatatt tttatttctt ttattaaaaa ttggaaaaac
    12541 tatataatat atattataat ctatatataa ggagtgatgt tattgaaaaa agatagaata
    12601 actttttcag aaattttaat attaattgta ggctgtatat taatggccat ttctcttaac
    12661 ttatttttta atccacatgc aatcgctgct ggtggtataa caggacttgg agttgtttta
    12721 aattcattat tcggtgtaga actttggatt gtcaatttac ttttaaatgt tccattattc
    12781 atatttgctt acaaaatact ttcaaaaaaa gattgtttta aaacggtttt aggaataatt
    12841 tttcttacca tagcacttaa gcttactgca aatatggcta cattagatat aacaaatgat
    12901 atgtatcttg caattatatc tggttctatt ttaatgggag ttggtcaagg acttattttt
    12961 agaataaatg gctctacagg tggaactgat ttaatggcac tcctactaaa taaatatttt
    13021 cctacattta gtatacctgt tttaatgggt attgtagact gcgttgtagt tgttttatca
    13081 ggtatagtaa atagacaggt tgaaattgca ctttattcaa ctgtggcttt atacatttta
    13141 gtaaaagtta gtgacttgct tattgaagga tttaattatt ccaaatcatt cactattata
    13201 tctgaccttt caaaagatat aagtaaaaaa ataatggaag atttagatag aggtgcaact
    13261 attctaaaag gagaaggtgc atatactgga gaaaataaaa atgtactttt agtagtagtt
    13321 gaaaagaaag aagttgtcga attgaaaaaa ttagtaaaaa atgtcgaccc taatgctttt
    13381 attataatta cagatataca tgaagcttta ggtaatggat ttaaaaaaat agaataacta
    13441 acaaataaat agcaagtata attaacatat acaaattcaa ctttttatat gttatattat
    13501 acttgctatt tatatattat acttgctatt tatatattat aattgttgtt tttaagataa
    13561 aaattaaatg tatcaattat tgttttatac aagtatttcc aaatccgttc tcatttagat
    13621 agtatgctta gaaattttag aattattgag tctaacttta ttacttactt tactttataa
    13681 caatcaaata caaatttaaa tattataaaa ctacaaaata atttaaagca tataatgtca
    13741 atatgtttaa attataaaat attcatgtag ttatataata aataatttat aaatatagcc
    13801 aacttagcct aaatacgtat ataatatagc taatatggtt cttatacttg ataccatcaa
    13861 taaaactaaa aatataaaaa ggagaactta aaatgagtgt tagtttacaa aaaggacaaa
    13921 gagttagctt agtaaaagat aataatcctg taaaaaatct agtagtaggc ttaggatggg
    13981 atatgaataa attgggtaaa aaaaattatg acctagatgc atttgcaata gcattgacta
    14041 accaagataa aatgaatgtt agaggtgact tggtttattt tggaaatctt aaacaccctt
    14101 ctaaagctat tatacacact ggagataact taactggaaa aggtgaaggt gatgatgaac
    14161 aaataattgt taatttagaa gacatacctg aatatgtaca taaaatagtt tttggagttt
    14221 ctatatacaa agctaaaaaa agagaccagg attttggtca aatgaacaac tcttttataa
    14281 gattaattga ttcaaattcc aagcaagaat tatttaaata taatcttcaa gaagactttt
    14341 ctggtaaagt aacagttttg gcaggagaaa tttatcgtag aaatgaagaa tggaaattta
    14401 atgctttagg aattggtcaa aatgaagagc ttcgtgaatt aattaataca tataagtaat
    14461 aattacaata aaaaatttat aatgaggagt tggtttaaat gggtataact ttagcaaaag
    14521 gacaaaaagt aagtttaaca aaaagtaacc ctggtctaaa aaaagtaata gtaggactag
    14581 gatgggatat taataaatat gatggtggct ttgatttcga tttagatgcc tctgcatttt
    14641 taactggaac tgatggaaaa gtaacgaatg atggagactt tatattctat aataatttaa
    14701 aacacgcttc tggagctgtt gaatatatgg gtgataatag aacaggtgat ggtgatgatg
    14761 aacaaataaa tgtagactta tctaaaatac ctcaaaatat tgctaaaata tcatttagtg
    14821 ttactataaa cgaagcaatt acaagaagac aaaatttcgg acaagttgaa aattcatata
    14881 taagaatata taatgaagaa actaatgagg aacttattaa atatgaattg ggtgaagatt
    14941 ttagcataga aacagcaata gttgtggcag aactatatag acataatgga gaatggaaat
    15001 ttaatgcttt aggttctgga tttgaggatg gacttgcagg attgtgtaaa aattttggcg
    15061 ttaatatagg ctaatatatc taaataaaaa taagagtata ttgatatact cttattttta
    15121 tttagatata ttataaactg tttacacttc tactcttctt tctcgcttgt tttttcccat
    15181 attctactga aatatcaaaa aatgctgaaa tattttctgt aaaaaacttg cttctatgat
    15241 atgcaatatc aaatgtccta tgtaaattaa taccttcaat atcacaagcc cacaaaagat
    15301 gcttttttaa ataatcctca ataagtaatt cggatataac tgcaatgcca aaattatcta
    15361 caactgctct taatatagct tcagagttat aacatgacca tctaatattc attgggattt
    15421 ttaattcttt taattgcttt tctaattgtg ctcttgtacc acttcctaat tcacgcataa
    15481 ttaatggttg atttgaaagt tctgaaactt ttatagagtc tcttttataa aactcatgct
    15541 tgtgtgaaca tataagtaca agattatcat taataatgct ttttgtaaca atatctgggt
    15601 gcttaatttt tccttctaca atcgcaatat ctaattcact attaagtaat ttttttgcaa
    15661 tcagtcttgt atcttctaca aatacttcag gttctataag aggatttacc ttgtataatt
    15721 ctaacattat tggacttatt acacatgtac caacagttac tgttgcccca actcttatac
    15781 ggatattttt tgcacaatgc ttcatacaat cattcatttc ttcatttaca gctaaaagat
    15841 gcctcccata tccaagaagt tttttccctg cctcagttaa atataatttt tttgaaattc
    15901 tatcaaatag cttaacacca tactcttttt ctatttctga aatagcttgg ctaactgatg
    15961 actgggtaat atacatattt cttgctactt cactcatttt accacattca gcaaccttaa
    16021 caaagatttc aagactacga attgtcatat tatcacaacc ttagcaaaca ttagtaaatg
    16081 cttatgattt tattaatatt ttagtatttt acaaatatat tgtcaagaag tataatttta
    16141 ttatagagtt ataaagtaga ttggagtgtg atatattgtg aaagtattaa ttataggtgg
    16201 agttgctgct ggaacaaaaa ctgctgcaaa aataaaacgt gaaatgggtg ataattgctc
    16261 tgttacaata ctaaataaag gtgatgatat ttcttatgca ggttgtggtc ttccatatta
    16321 cgttggtaag ataattgaag ataaatcatc tttaatcgta aatacacctg aaagtttttc
    16381 taaactaaca ggagcagaag ttcgttgtgg agttgaagtt acatctattg atagaaatgc
    16441 aaaacaagta actttgaaaa cgctttcaac aggagaaaaa gaaaatctaa gctatgacaa
    16501 acttgtaatt gctacagggg cagacccaat aaaaccacca atacctggaa tagatttacc
    16561 tggagtattc tttatgcgta ctccaaatga tgctataaaa ctaagagatt ctattgaaaa
    16621 cggtattaag agagctgttg ttattggtgg aggtttcatt ggtcttgaaa ttgctgaaaa
    16681 tctttctgca atgggtatac gtgtaagtgt tgttgatatg gctgaacatg ttatgcctgg
    16741 atttgatact gattttgctg aatacgttga aaatcatatg gctgataaag gcataatgat
    16801 ttttacaggt gaccaggttg taggtatcga aggtgaaaat aaagttgaaa aacttcgtac
    16861 taaaaataga gctattaaaa cagatttagt ggttatgtca gttggaatcc gtcctaacac
    16921 aggttttcta aaagacacag gtttagaatt tgcccctaat aatacaatta ttgcagatga
    16981 atatatgctt acaaatgaca aagatatata cgtagttgga gactgtgctt ttgtaaaaaa
    17041 ttctttaaca aataaaccta catggtcacc tatggggtca tctgcaaatc atgagggtag
    17101 aatttgcgca caaaatatat ctggaaaatc taaaacttac aatggtgttc ttggaacaac
    17161 agtagtaaaa ttacctgagc tcaatgcagg aaaaacaggt ctaagtaaag aaatagctga
    17221 aaaagaaggt tataatgttt gctctgtaac catagctact gatgataaag ctcattatta
    17281 tccaggtgca tctaatttta ttataagaat ggttgctgaa aaaaatacta gaaaattatt
    17341 aggtgttcaa gttatgggac ctggagcggt tgataaaata gtagatatcg ctgctactgc
    17401 aataacttta ggcgctgacc tttattcatt agaaagtatg gaccttgcat atgcaccacc
    17461 tttttcaaca gctattcacc cttttgtagt ggcagtaaat gttttacaaa ataaacttaa
    17521 tggagaactt gatagtgttt tacttgacga aattgaggat tttgacagtt ggactaaact
    17581 tgatgtttca aaagctcctt ctattcctag tttacgttat attcctgttg gtgaaattaa
    17641 tggagaaatt gaaggccttg ctaaggatga aaaaattctt cttatatgtg ctaaaggtaa
    17701 aaatagctat atggcgcaaa atcgcttaag acgctttggc tatacaaata cgaaagttct
    17761 tgaaggtgga atactatttt atccaaatct taaggtaatg gaggagtaaa tatgagtaat
    17821 gttacagcag aacaaaaaaa ggaagtaaaa ggtagaggtt ttctaccaag cagggatgga
    17881 gaacattttg cagcacgtat aataactgta aatggtgtaa taaatgcatc acaagcaaaa
    17941 aaaattgcag aagctgctga aaaattcgga aacggtcagt tagcatttac tactagacta
    18001 acagtagaat tacctggtgt aaaatttgaa gatattgaag ctctaagtga atttattgct
    18061 agcgaaaatc taataacagg tggaactgga tcaagggttc gtcctgttgt ggcttgtaaa
    18121 ggtactgttt gtgttcatgg tctagctgat acacaggcac ttgctgctga aatacacgaa
    18181 gaattttaca aaaattggta tgatgtaaaa ctacctcata agttcaaaat tggtgttggt
    18241 ggatgcccta ataactgtat taaacctgac ctaaacgatc ttggaattgt tggtcaacgt
    18301 gtacctgact atgattcaga gctttgtgtt ggttgtaaaa aatgtgctgt tgtagaggtc
    18361 tgtcctgtta aagcagctaa attaacagat aaaggtaaac ttgaaataga tagtaatcta
    18421 tgtaataact gtggtaagtg tatagaaagt tgtaattttg atagtattga agaaaaagaa
    18481 tctggttata aagtctatat aggagggaaa tggggaaaat cagttcgtcc tggaacacag
    18541 attgatagat tattttcaaa agaggaactt atgactttaa ttgaaaaagc aattctttta
    18601 tttagagaac agggtaacac aggtgaacgt tttggaataa ctatcgatag aatcggtgct
    18661 gataaattta tagaaatgct tttatcagat gaagttttgg aaagaaaaga agagatacta
    18721 gaggctcttt tacaccttac aggtggagct gtatgctaac aataaatatt ttaattgtac
    18781 agataaccta tggaaaaaat tttagttaag aataattaaa attctatata tgatatatga
    18841 taaatgaggc tatctcaaaa cacagatacg cctctttttc attgttgaaa tgataaagta
    18901 tatttgagtt catataaaaa agagtgtctc gtgaattata aaattcattt tgaggcaccc
    18961 acattatttt ataataattg cttattaatt ttattttaac tatataaaaa gtacaaaaag
    19021 taagaatatc caatattcta aaattaattt tgaattgtat tgattgaaaa gtttactatt
    19081 ccactatttc catcttgatt tactactaga taccatgttc cagtattagg aactctcatt
    19141 ctacatggat ttttctttgc aaatccacca tagtaatcat aatttctttg atttttaaat
    19201 ttcacataat ttatatggtc taacaatatt acattggctt gtttctctaa atttacctct
    19261 acaatatccc ctttttttaa gttcatctta ctataactat attccattga attactcctc
    19321 cagtatcgaa taatctttgt tatttactca tctcaaatat gtaaaaattc atttaaaata
    19381 ttacaaatac tatataatta gactattatc cagttttaac cttatttatt acaatccaaa
    19441 ggttattaaa aatattattg gcaacaactt ctccatccca tacaactctt tcttctttat
    19501 tttctaataa agcatagtac ccagagtcag aactttcatc tttatagact gtaacataaa
    19561 actcttcatc actatattca ggtatatttt taaagtcttc agatatttta catgttaata
    19621 tataaccttc taattcttca tcta
//
LOCUS       DAEMGG010000071        19381 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEMGG010000071 DAEMGG010000000
VERSION     DAEMGG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.071"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..978)
                     /locus_tag="KPK24_002332"
     CDS             complement(<1..978)
                     /locus_tag="KPK24_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG2300001.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNE"
     gene            complement(1026..1886)
                     /locus_tag="KPK24_002333"
     CDS             complement(1026..1886)
                     /locus_tag="KPK24_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG2300002.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2155..2364
                     /locus_tag="KPK24_002334"
     CDS             2155..2364
                     /locus_tag="KPK24_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG2300003.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2364..2630
                     /locus_tag="KPK24_002335"
     CDS             2364..2630
                     /locus_tag="KPK24_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG2300004.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2659..3231
                     /locus_tag="KPK24_002336"
     CDS             2659..3231
                     /locus_tag="KPK24_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2300005.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3466..4656)
                     /locus_tag="KPK24_002337"
     CDS             complement(3466..4656)
                     /locus_tag="KPK24_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2300006.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4916..5347)
                     /gene="dut"
                     /locus_tag="KPK24_002338"
     CDS             complement(4916..5347)
                     /gene="dut"
                     /locus_tag="KPK24_002338"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG2300007.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5460..7688
                     /locus_tag="KPK24_002339"
     CDS             5460..7688
                     /locus_tag="KPK24_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG2300008.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7799..8284)
                     /locus_tag="KPK24_002340"
     CDS             complement(7799..8284)
                     /locus_tag="KPK24_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2300009.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8536..9759
                     /locus_tag="KPK24_002341"
     CDS             8536..9759
                     /locus_tag="KPK24_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG2300010.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9763..12897
                     /locus_tag="KPK24_002342"
     CDS             9763..12897
                     /locus_tag="KPK24_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG2300011.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12931..14229
                     /locus_tag="KPK24_002343"
     CDS             12931..14229
                     /locus_tag="KPK24_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG2300012.1"
                     /translation="MSKIVAISCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14518..14763)
                     /locus_tag="KPK24_002344"
     CDS             complement(14518..14763)
                     /locus_tag="KPK24_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2300013.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(14977..17604)
                     /locus_tag="KPK24_002345"
     CDS             complement(14977..17604)
                     /locus_tag="KPK24_002345"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG2300014.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17665..18507)
                     /locus_tag="KPK24_002346"
     CDS             complement(17665..18507)
                     /locus_tag="KPK24_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG2300015.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18514..19152)
                     /locus_tag="KPK24_002347"
     CDS             complement(18514..19152)
                     /locus_tag="KPK24_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2300016.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
BASE COUNT         6759 a         3087 c         2679 g         6856 t
ORIGIN      
        1 ttcattatct aagtcttcat caaacatttt tattatattt tttatctcat acttgtactc
       61 atcttcattc attctataaa gcattaaatc ttctagtaca tctttcatat cgaagtacac
      121 atcttctaat aaataaaaat agccttcctc tatatctttc aatttatcta tagtatccat
      181 ttttttactt atatctttca taatttctaa attttgtata tttacatcta tgattgttaa
      241 taagtacatt ctcacagttt ttattatttc aacagaaaaa tctacaatat tataatttcc
      301 ttctttttta tataatgact ccttaaattt gttaaaacaa ttatctaaat tagaatttat
      361 atcgttaaat ggtggtctta ttatgtcacg tattattaaa agattttgta tatctacatt
      421 atttgtgatt gcctcatcca aaataaataa attatatgca tcatttaatg ggtcatgggc
      481 atctccacta aactctaagt taacaaattc aagtaaattt ttcaatgata tatagttcat
      541 acatctcata ccacaattta tatatttttc ttttatatct acaaataaat ctctaatgtt
      601 atttacaaac cttggatggt tatttttttt agcacttctc atatctgtgt tattgaaaca
      661 tgtcaaatcc aaattaccaa aagtatatat tcctttaata tcagaaaata tgccaagcca
      721 ttttttaaac tttctcatga cctcttcata acttggtgca ctcttcaatt cttcagaaga
      781 tatatgtgtt aattcttgta tatgaggata caactcctca ttacttactg gtcttattaa
      841 agatttaaat ttatcaatat tttttgtttt ttcatcatac ataactgctc cgattgcaat
      901 tatatcagaa ttaaatactg ctctttttga tttagaattt ggcatattca tttcaaaatc
      961 tatatatact cttttcaata taaacaccct ttccaattgt ttatagtgat tctttttaac
     1021 tatttttatt caaaattttc taagaaagaa tgtttctccc catctttttg ccaaccaagt
     1081 actgaatcaa gatttacatt ttctgccgcc ttaaatatag atttttctac atttttaaaa
     1141 tttgattcta agctctttat taagtctttt atctcatatc ttttattacc tgttttttta
     1201 gcagcaacca tacatgcaca atttaatggc catataccac tattttgaat aaatcttata
     1261 atatgttctt ctcttatata ataaagtggt cttataattt ttataccttc aaaattagta
     1321 gaattaagtt taggaagcat tgttttaaaa tttccagcac acaataaatt aagcattgtt
     1381 gtctctatta catcgtcata atgatgacct aatgcaagtt tgttacaccc taattcttct
     1441 gcctttgaat ataatgctcc tctcctcatt cttgcacaca tatagcatgg ataatctttt
     1501 gctatttcat ctgctatctc aaatatcctt gaatcaaata aatgtattgg tatatttaga
     1561 tactcacaat tatctataag taattgtctt atatttgcat gatatccagg gtccatagca
     1621 ataaattcaa catcaaaatt aacttgtcca tgttttttta actcttgaaa cattttagcc
     1681 ataagaatac tgtcttttcc accagaaatc gctacagcta tcttatcacc ttcttcaacc
     1741 aatttataat ctcttattgc cttcataaac ttagaccata aatttttcct atattttttt
     1801 attatacttt tttctatatc tcttactgga agtctttcct caaaaggaac tataatttca
     1861 cagccttttc ctgatagttc actcatctaa tcacctcgta tatttatgtt aataatattt
     1921 tacaaaaaag tctaccttca tccacaaaat actataattt ttactttaat tttactaaat
     1981 aatttatagt ttacaataac acataaactt ataataacat aaataacctc tttcttttat
     2041 tatctttttt atttttctca tttaatattt tattattagt aacgattttt aaatgattga
     2101 atattataca ctagatagag ttacctatct atttacaaca aggaggtttt catcttggaa
     2161 aataagacta gagatatcaa aaaaaattgt ggtccatctt tggctagacc atacattaat
     2221 aatcaaattt tcacagaaat gtactgcctt tctgatgcat taaaatttgg aactatattt
     2281 ccagaactaa acttactaga gtctgaaatg tacaacaaag aattatatgc taagccaaaa
     2341 aaatatagag gaggtagaag ataatgaaag actcatctag agctgaactt atgagaaaag
     2401 ttcaggaaac atcttttgca tgtgttgata tgaacttata tttagacaat caccctgatg
     2461 ataaaaatgc tatcaacact tataattcat tatgtaatca atttgcgcaa gctagatatg
     2521 cttacgaaaa taaatacggt cctttaacta actttgggta tgctccaagt agatgccctt
     2581 ggcaatgggc tgaccaacct tggccttggg acagagaatt taattattag acatcaagta
     2641 ttggaggatt gaaaattcat gtggatatat cagaaaacta tacaacaccc agttaatata
     2701 aaaacttgtg accctagaat ggctaaattt cttataactc aatttggtgg gccaaatgga
     2761 gaacttgctg catctttaag atatttaagc caaagatata caatgcctac tggtaatatg
     2821 cgtgcacttt taacagatat tggtacagaa gaactagctc acgttgagct tatatgtact
     2881 atggtttatc agttaacttc tgatgcaagc ccagaagagt taaaagctgc aggtcttggt
     2941 tcaaactatg ctcaaaatgg atatggaatt tatccaacag attcaaatgg tgttccattt
     3001 gatgtaagac ctatagcagt tatgtcaaat cccgtaaccg atttacatga ggatatggca
     3061 gctgaacaaa aagcacttgc aacttattat caacttataa acctaacaga tgacgttgat
     3121 gttatagatg tattaaaatt cttgggtcaa agagaaataa ttcattatca aagatttggt
     3181 gaagccttaa tggatgctta cgagttagaa gaatctcaaa aaatgttcta aaatttttat
     3241 ttgtataact attattattt gataatttat aatttactgt tgtgttaaaa tttataaaaa
     3301 atataaatat aatttatttt ctataaacag agaaaataaa taaaaagcag gtatcaatca
     3361 aatagtaaca tgtattatac tatacattac tatcaatatt gtacctgctt tcatattata
     3421 agcaataaag aaaaatctat ttattaggtt ttataataaa aacaattata aaactcttct
     3481 tgcatttaca tatctacttg aatagtagct actatttata ttagatattg ttactttttt
     3541 tgatgaacct gaagatgcat gtatcatatc tccatttcct atgtatattc caacatgact
     3601 tacagaacca ttatttgaac cttgtgtatc aaagaatact aaatctccac ttctaaggtc
     3661 tcctctattt acatatgttc catacttact ttggtctcta gatactcttg gtatactaac
     3721 tcctacagat tttttcataa cgtactgcgt gaatccagaa cagtcaaaac tatttggacc
     3781 ttctgctccc catacatatg gttttccaag tagtgtcttt gcaaaactta ttacactatc
     3841 agctccttga cttggagaat tagtcgaata agatggaatt gaagtatcaa cagaaccttt
     3901 ttcagcaagg tattgacttg acacatatcc agtttgattg ccataactta tttttgtcca
     3961 tcctccactt tcagaaatac ttccaacctc agtaccataa cttaatgtag ctattttaga
     4021 atgacttgta cctggtcctg ttctaacgtt caatgacttt gctgttacaa cctttgtacc
     4081 ctttacaagg tcatttgaac tgttttcagc attttcattt gtagagtcat ttacactagt
     4141 tcctaaatat ttacttgaaa cataacctac tctaccatta gaacttattt ttgaccatcc
     4201 atttgactca gatatgtatc cgacttcagt cccatatcct agtgttgata ttttagaact
     4261 acttgtactt ggtcctgttc taaaatttaa tccttttgct gttacaactt ttgtcgactt
     4321 aactgaagta tcactacttt cattgctact tccaaggcta tttattgcta cataatttcc
     4381 atgcacataa cctgtgtttc cattatattt aacttttacc caagaacctt cttttcctac
     4441 gtactcaact ttatcacctt tgtaaagttt ccccattgat tcatggtttg tacctggtcc
     4501 ttttctaaaa tttaccgcat tccctgttat tgttctgctt tctatactca cactctctac
     4561 ttcagactca ttagcatatg ctaaattccc catagtaggc attattatcg cagttgccat
     4621 tatactggct gctattctct tttgattaaa attcataact ttccactcat ctcctctttt
     4681 ctactatctt tatttcactt caaaatataa aagaaataat tgacactatc aaaatatcaa
     4741 ttatttcttt tatattaata ttattacaaa tttgtaacta tttgtctata gtaatatttg
     4801 cttaccataa attaccatgt ataaatttta atctttaata gcctgtaatt tagtaatata
     4861 gccatctttt acctttttta aaaatggcta tattttttaa cttttttgta actttttaaa
     4921 atccagttga gccaaaccca ccttttcctc tttcggagtc acttaattct tttacttctt
     4981 ctatgttaat atcatagata ggttttacta tcatttgagc aattttcata tgcttttcaa
     5041 ccttaaaatc atttttacca tgatttatta atataatttt aagctctcct ctgtatcctt
     5101 cgtcaatagt tcctggtgta tttaatacag taacagaatg ctttaaggca agcccacttc
     5161 taggtcttac ctgcgcttct gtcatagtag gaagctctat acatatccca gttttgataa
     5221 gtttagtctc tccaggaggt attacaacct cttctataga atacaaatcc ataccagcat
     5281 cacctttgtg agcaaaatta ggtattatag catcatcatt taattttttt acttttaaat
     5341 tgtacatctt atatctcctt tcaactttct ataaatatta taacttaaat ttcgacctct
     5401 tgaagttttt tatttttttg tggtaatata attattagtg ttttaaagga ggatttttta
     5461 tgaaacttgt acttgctgag aaaccatctg tggcaaaaac tatagcatca tttttaggtg
     5521 caaagaccag acaagatggt tactttgaag gaaatgatta tataattact tatgcagttg
     5581 gacacttagt atcattatat gatatgaagg attatgataa agataaatac tctggctctt
     5641 ggaaaatgaa caatttccca tttgttcctg aggataagtt taaatttaag attgatagtt
     5701 ctaaaactaa acaattcaac actgtaaaaa agcttttgaa tagaaaagat gtagaatatg
     5761 taataaatgc cactgataat gaccgagaag gagaacttat agcattttta atatttttac
     5821 tggcaaaaaa taaaaaacca gtaaaaagaa tcttggtaaa tgaatggaca ccagaagata
     5881 ttacaagagg tataaaaaac ctaaaagatg aagatgaaat gagaaatctt caagctgctg
     5941 gatatactag gcttattact gactggttga ttggaataaa tttcacttct gttgctacat
     6001 tgaaatacgg aaatgggaaa ctattaaaca taggtagggt aattcttcca acagttaaat
     6061 tagtctatga tagagatatg gaaatactaa actttgttcc taaaacttat tatgaaatag
     6121 aagggcattt taaagctgaa gctggtgaat ataaaggtaa atatgttaag ggtaaagaaa
     6181 gtaaatttga tacacttgaa gatgcaaata aaattatagc ttcaattaca tctgaaactg
     6241 gtaaaatact agataaaaaa gttactatgt caaaggaata tgcaccaaaa ctttttagtt
     6301 tgacatcact tcaaggatat ataacttcta agtattccaa ttttacatct gataaggttt
     6361 taaatgtctg tcaatctctc tatgagggta atggtaaggg tggatacata acttacccta
     6421 gaactgattc cattttctta gaagaaagtt tggcttcaaa agcttctcaa actttagata
     6481 aattaaaggt tggtcttgaa tatgaaaata aaattaaatt ttctaaaact aaaagagttt
     6541 ttgattcttc taaagtagac tcccatagtg ctataattcc aacttatata attccaaaaa
     6601 gtttatctaa ggatgaacaa ttagtttatg atgccataaa agatagattt gtagcaaact
     6661 ttatgcctcc tgctgaatat gagaatacag aaattaagac tgaagtagat aattgtactt
     6721 tcttaacaaa aggtaaggtg cttaaatcaa aaggatacct tgaggtatat aataaggaag
     6781 aaaaaaacga tttacttcca cttgtaaata aagatgatgt tgtagatgtg ttagaaataa
     6841 aaccattaac taagcaaact actccaccta aaccgtatac tgaggacaca cttttaaagg
     6901 ctatgaaaaa ttgtggtaaa aatgtgcctg aagaagatac aacagtgcta tctggatatt
     6961 cgattggaac atcagccaca cgtgctgatg tgttaaagaa gataagtcaa gtgggctatg
     7021 taaagaaaaa aggtaaatct tattctataa cagaccttgg gaaaaattta gttgaaattt
     7081 ttcctgttaa agacttattt gatgtagatt atactggaaa acttgaaaaa agtttaagcg
     7141 atattcaaaa aggaaagtat acaagaaaag aatatcttac taatatcatt agttttatat
     7201 atcaaaatgt caatattata aaacaagatg cttcaaaaaa tataaacaca gaagcatata
     7261 ctttcgattc aaaaactaaa aaatttacta aaacaaaaga aaaaaaacag aaaaaagatg
     7321 taactaatac tgaaaaagaa gttgctatat caaaggattc taataacaaa aaagaagtta
     7381 aaaatggtga tgaaaatcaa tctcttggca aatgccccat ttgtcaaggt gatgtactag
     7441 aatttgacaa aggctttgct tgtaaaaatc ataaagaatg taaatttgtt atttggaaaa
     7501 atgataagta tttaagtctt tataaaaaga aagttaacaa aacaatggtc aaaaatattt
     7561 taaaaaaagg tgaaactaaa gtaaaatctt taactgctaa aaacggtaat aaatttgatg
     7621 ctacattaaa atatgataaa aatccagata ctggctattt caattggaaa atagagtttg
     7681 acaactaaat attcataaga aaagaaatag cagtagtata ataatctata ttatattact
     7741 gctatttctt ttcttatgaa gtttcaactt ttttaatagt gacttttaca atcacactct
     7801 aacctaattt ctttactatt tctgataact ttaatgtatg ttcatctaat aaagttagtt
     7861 cctcttcaga aagattagat aattttttta ctagagattc tttgagaata gcttttactg
     7921 catcaaataa tttaaatgct ttatctgtta attcaaccct aagaatcctt ctatcttttg
     7981 ggtcaggaaa tttattgact aaaccatgtt ccactaatct gtcaataatc ggagtcatat
     8041 ttgattttaa aattcctaaa ttagaagcta tttgagaaat tgatgatgaa tttgatgtca
     8101 taagataaag aattacctta gcatgtgatg gagccattgg acattcttca aaataagctt
     8161 tcattatatc agagttattt tgaaactttt ttaacaaatc gctttcttta aatatattat
     8221 tttcaagcac aaataaaaaa tctaaaaaag agtccgctat tttaagtata ttgttgttat
     8281 ccatatttgt aacatccctt caatagataa tataataaaa cattgttgtt taatatagta
     8341 taaattataa actattaata aatttaaaac aagaaaaagt tattgactca taataattat
     8401 aaatttataa tcaaattaga gaattataaa ttgccaataa agggaagttt tttaattagt
     8461 ctaaaactaa acttaaatta taagacactt taaattctac aacaagctag tgtgcaatat
     8521 ataataggag gagatatgaa aagatattta atagttttct taatgtgtag ttcactgttt
     8581 ttagttggat gtggaaaaac agaagaatct gaacctgaga aaccaatagc agtttctgtt
     8641 caaaaagcaa taggtggaga aattgaaaac acaaattctt tctcaggaac tacaaaggtt
     8701 aaagatgaaa cagctgttac tgcacagact gttggcacag ttcaagaagt gtatgtaaaa
     8761 cttgggcaaa atgtaagaaa aggagatgaa ttattgagta taagcagtcc agagctagaa
     8821 aatagtgtaa agcaaagtaa agcctcacta gatttagcaa aagcaagtta ttcaagtgcc
     8881 actggtggtt cattagaggc acaagtaaat caggcaaaaa cagctcttga caatgcaaag
     8941 attcaatatg aggagtctca aagaaattat gacaataata agattcttta tgaacaggaa
     9001 gtcattagtt tagaccaatt taaaaaaata gaattttctt tagagcaaac aaaacaacag
     9061 ttagattctg ctcaaagagc atacgataca gcaacttcaa aaagtatacc tcaagctaaa
     9121 gcacttgcta aaaaacagct tgaccaagct caagtatcat ataatttagc aatgagtaat
     9181 ttagacaaat taacattaac atctccagta gatggtacaa taacagctaa aaactttgat
     9241 tcaaaggaaa tgataacaca atcacaacca gcttttatta tttctaatcc taatatacta
     9301 gaagttgatt taaatgtagc agaatcagat ataggtaaat ttaagaaaga tggaaatgta
     9361 gatgttataa ttgaagacca aaggatttta ggaaaaatag attatgtacc ttcagtagtt
     9421 gaccctcaga catctttata tcctgttaaa gtacttgtaa ataatacaaa caacaaattt
     9481 aaggcaggta tgtctgctca agtaaactta agtgttgaaa aagaaaatgg tgctgttaca
     9541 gtacctaaaa aagcaatatt tgaagaaaat ggtaaaaaat atgtatacat agcaacaaaa
     9601 gacaatatag caaagaaaca cttggttcaa actggaattg taacagagga taaaatagaa
     9661 attaaaagtg gtgtaagtga taaagataca gtgattatcg gagggataag tcttatttct
     9721 gacggaacta agatatttcc tgtagaaaag gagaagtaga ctatgaattt aacacagact
     9781 tccgttaaac gtcctctcac aatcataatg gtctttttag tattaatagt ttttggtggt
     9841 attggatata aaaaaatgtc aataaatcta atgcctgata tagaaatacc agttgtaatg
     9901 gtcatgacta cttggacagg tgctggtcct caagacgttg atgaacaagt tagtcaaaaa
     9961 gttgatgaaa gtttatctgc cgtttcaaat gtaaaatcta ctatatcaag ttcacaagaa
    10021 agtgtttcta tggtagttgc tcagtttgaa tttggaacta atttggatga aataatgaat
    10081 gatgttaggt ctaaagttga tgcattacag accagcctcc cagatgatgc agctaaacca
    10141 acagttttaa agctggatat gaatgcacag gctataggtc aacttgtcat atctggagga
    10201 aatgaaaata gttcacaagc cttaagaaaa tacgctgaag atgtaattca gcctaaaata
    10261 gaatccatag atggtgttac ttcagcagac cttaaaggtg gagaaaaagc acaggttaat
    10321 gtaatagctg acccagctgt tctttcaaat tatggagtta gtttatccac tataaaaggt
    10381 gtcttatcct ctagtaataa aacattccct tatggttcaa ttactcaagg tgaggataaa
    10441 atagttttaa gagcgattga taaattagaa tccctagatg atataaaaca gattcaaatt
    10501 cctgtaaaag gaggaaatac agttagatta gataatcttt gtagtgtaga ttatggatat
    10561 gcagacaaag atagtattta tagatacaat ggaaaagaca gtttggtaat agatatttct
    10621 aaacaacaag atgccaacac tgtaaaagtt atgcagagtg catacaaata cgtagatgaa
    10681 ttgaataagg aaaatcctca atttaaaatt aaaattgcta atgatacaag ttcatacata
    10741 agtgaatcaa taaacagtgt aatgagcaac ttgcttataa gtgctgttat cgcatttatt
    10801 gtaatatttg ctttcctaaa aagtgtaaga gcttcactag ttgttgcagt tgcaatacca
    10861 atttctataa taggtgctat agcaattctc tactttactg gtgaaagttt aaatcttatt
    10921 actgcaagtt cattggtaat ttcagttggt atggttgttg ataatgctac agttgtaatt
    10981 gaaaatatat ttaaatatag gaaaagtggt agattgagtc ttgatgattg tgctattgag
    11041 ggaacaagaa ctgtaacaaa tgcaatactt gcatcaacat tgaccacaat agcaatattc
    11101 ttacctatca tgtttacaga aggtatagct aaaataactt ttggggcatt aggtaaaaca
    11161 ctaatagctt cactagcatt ctcctttata ggtgcaatta cactcgttcc tagtgtattt
    11221 gcaaaattaa gcagaggaaa aaatagtcaa aagatgcaag aaaaagatag tcctatattt
    11281 gataaggtaa gtgaagcata taaaaaatta ttaagtgtat gtttgagaca tcaacgctta
    11341 gttgtaatat caagtttact tatttttgta gcttccttat ttggaatggg atttataggt
    11401 atggacttta tgcctgcagc agacaagggt gaattatcta tttctataga acttccaaaa
    11461 ggtcttagcc tagaatccaa tgattactat gtatctatga ctgagaaaaa agtctcagat
    11521 attccagaaa ttaaaacttt aataacaact ttgacatcag attcaaataa tagtaaagca
    11581 agtatagcaa ttgaattaga atctcaaaaa aatagaaaaa gaactactga tgaaatagaa
    11641 aaagagataa ttgatagagt ggctactgta ccagattgta aaataaatgt aagtcaaaac
    11701 agtagtgtta tgggtggagc tggaggagca gacttcacat tagaaatgaa aggtcctgat
    11761 ttagatgtat taaaaatact gggaaaagaa gctgaaaaag aatttagctc tataaaagga
    11821 tttagaaatg ttgaaacttc tcttacagat acaacacaag aagctcaatt tactatagat
    11881 aaacataagg ctcaagaata tggtgtaaat accgctgaaa ttgcaggcat gttacgtaca
    11941 gcaataaatg gtgactccgt aaccactgct actattgatg attacaaagt ggatgtaaac
    12001 ctaaaattta aagcagatac tattgatagc ttagaagata taaagcaact aaaagtaaac
    12061 tctgcaactg gaaaagaagt tcctcttggt gccttttcag atataaaaat ggcagatggt
    12121 ctacaatcta tatctaggac tgatggagat ttttctgtaa atataactgc tacacttgac
    12181 aatttagatg ctggtactgc tacaaaacta gcactagaaa aaacgaataa aatggtaatt
    12241 cctaaagatt actctatagg tgttggtggt gaaacagaaa tgatgaatga atctatggca
    12301 agtcttggat tttcaatggt catagctata gcattagttt atatggtaat ggtagctcag
    12361 tttgaatcat ttagtaaacc atttataatc atgttctgtg ttccatttgc attcgttgga
    12421 gttgtgcttt cgcttctagt tagtggtcaa agtttaagta ctgtcggtat gctaggtata
    12481 atcttactta tcggtattgt tgttaataat ggtatagttc ttattgacta catagagcaa
    12541 cttagaaaaa caaatcgtaa caatgattta attggtttgg tttcaattgg ttgtgcagca
    12601 agattaagac cagtcttaat gactacagca acaacaattt taggtatgtt gccaacagca
    12661 ctatctcttg gtgaaggtgg agaaactatg caaccactag ccatagttat aatgggtggt
    12721 ctaactgtat ctacacttat aactttggtg cttataccta ctatatactt aatatttgat
    12781 aaatgggaaa ataagttttc agacaaaatg cctaaaatat ttggtaagat aacagataaa
    12841 ataggaagta taaaacgtaa aataataaag aaaccaaaat tgaaagctaa agtttaaata
    12901 gaatggaggg tgattgtatt gaataaaaaa atgagcaaaa tagttgcaat tagctgtggt
    12961 gttggtataa taactgccaa tttaatccca gtttacgcag ataataatac ttcttctata
    13021 aacaaagttg ataattctat tttaagtgac tcaaataaaa gaacatctga aaaggaaatt
    13081 ttgactgcta gagaagctgt tagtgctgca cttaaaaata gtgaaaaatt aaaaatgaaa
    13141 tcagaagaaa taaaaatgtt aaaagaaaag ttagaagtac aagatgagtt tgatagtttt
    13201 actggaagtg acaactcgtt tccatacgac caaattggac ttcttaaaaa tcaaagtgaa
    13261 caggctaaag gctttatgga ggaccaaata gcaaatgata ttactaataa atttaacgat
    13321 ttagtttcaa gagaaaatga gctagataaa ataaaaaaca atcttgaaat aaaaactaaa
    13381 gaaattaaag atatgaaatt aaaaaaagat ttagggttag taacatcttt agaaactgaa
    13441 tcagctgaac ttgaacttca aacactacaa aatactcaaa aagctaaact tcaagaacta
    13501 aaaaacaatc aagactattt taagttatta actaatatag atttaaataa ttatcaattg
    13561 gataaagaat atagatttga atcttttaga gtaggtggct cagttgattc atatatggaa
    13621 ggtaaggtta atgaatattt aaaatacgac caattgattt tagaacgtac agaagaaagc
    13681 tttaatgata aagatgagaa caaagcagat ttaccagata gacctaacct tacaagacca
    13741 gttgctccta caaaaccaga gcaaggtaac ctaactgatg atgaatacaa ggttcttatg
    13801 gataagtatg aaaaagatta tgatagatat aaaaaagaag ttgaagcata caatcttgaa
    13861 agacagactt atgctgctgg ccttactact tatgcaaatt atcttcaaca aaaatttaat
    13921 actgaaaatg gtctagtaac attagaagac agtaaaaaag ctcttaaaaa agggttaatt
    13981 gatagctatg ctcaattact tgctttagaa gatacaattc agattactaa aaaacaactt
    14041 gatttatcag aaaaacaatt aaaaaatact aaattaagat atgatttagg attaattact
    14101 ctaactgatt ataaaaaaca agtggtaagt aatgaagatg ctaaaaatag ctatgacact
    14161 cttattgtga attataactc actaaaaaat ggtatagaaa aaccttggat acttaatact
    14221 ggaaaataat tctggtaaat agaaagatta ctttaacata cgtttgatta aaaaatagtt
    14281 atgcatactc gtggctttag tcatggaaga ttcagaaggc aaataataga aatagattag
    14341 taaaaatata tttacttata aaactgtata cttttataga ggctatctca aaatagagat
    14401 aagcctcttt tttattgttt aaattataaa aatatatttg attccataca aaaagagtgc
    14461 cttctgaact aaaaagttca ttttgagaca ccctcaaaag cttaactcta aatttaatta
    14521 ttttttatca aatgtttcat ctattttaca tctcaccatg ttaacaaatt cagaaatatt
    14581 atatcctcta agtttgcatc cacatttagg acaataatcc agtttcctag ataccaaagc
    14641 cctacaacta ggttctggac atcttatttt tagactctca tctgctactt taaatccaca
    14701 tcgtgggcat atactacctg gacgtgatga tacttccttt ccacatttag gacactttgt
    14761 caactttatc ccctccagta aactcaattt aattgatagt tgaaatttct ctctacaata
    14821 atatatgcaa tataattaaa tacgtttcac ttacaactat ataagttaaa tttttataat
    14881 aaaaatcccc tatattcaat ttacaaatac ttaatttgat acattgaaca ataggggatt
    14941 ttatttttat ttagttacat attttgcagt ctattattat ctaggatttt ttatagatgc
    15001 ttgagctgct gcaagtctag caattggcac tctaaatggt gaacaagata catagttaag
    15061 tcctacacta taacagaact ctactgaaga tggttctcct ccatgctctc cacatattcc
    15121 tagttttaat tctggtttaa cttctctacc taatttagct cccatcttaa ctaacttacc
    15181 aacaccattt tggtctagta cttggaatgg gtctttttct aatatttctt catctacata
    15241 ttgacctaag aatttaccag catcatctct tgaatatcca aatgccattt gagttaagtc
    15301 attagtacca aaactgaaga agtctgcttc ttgtgctatt tcatcagcag ttaaacaagc
    15361 tctaggtatt tctatcatag tacctacagt ataaggaact tctatacctt cttcttttat
    15421 tatagcgtca acagtttcta ctatcatttt tcttattatt tttaactcat ttacttctcc
    15481 aactaatgga accattatct ctggagaaac tttaacacct gctttgattg cttctatagc
    15541 accttgtatt atagctttag attgcattac acatatttca ggataagtta ttgcaagtct
    15601 acaacctctg tgtccaagca ttgggttaaa ctcatctaaa tcaactattc tcttcttaat
    15661 atcacttact tttattccca tagattttga aagttcttct atagtctcat catcatgagg
    15721 caaaaattca tgtagtggtg ggtctagtaa tcttatatta actggttttc catccataac
    15781 tttaaataac tctacaaaat cctctctttg cattggtaat attttttcaa gagctttttc
    15841 tctttgttct actgtattag ataatatcat ttctctaact gctggcaatc tagcttcatc
    15901 aaagaacata tgctcagttc tacatagacc tataccttct gcaccaaact ctatagctgc
    15961 acgagcatct ctaggattat ctgcattagt tctaaccatc atacatttgt gtttgtctac
    16021 ccagttcatt aatttttcaa aatttccagt taaagatact tctgttttct taacatctcc
    16081 taggtacaca tttcctgtag aaccatctat agaaatgtat tctccttctt ttataactac
    16141 atcatcaagt gctcttattt ctttattaaa ctcatcaact tttatttctc cacatccagc
    16201 aacacaacat ttacccatac ctctagcaac aactgctgca tgtgacgtca ttccacctct
    16261 agctgtaagg attccttctg ctttaaccat tccttctata tcttctggag atgtctctag
    16321 tctaactaaa actacttttt ctcctttttc atttgctttt actacatcat cagcattgaa
    16381 ataaacttta ccacttgctg cacctggtga tgctggtaaa ccttttgcta taaccttggc
    16441 ttcttttaat gccttatctt caaattttgg atgtaataat tggtctaatt gattaggctc
    16501 aattctcatg atagcctctt tttcatcaat tattccagct tcaactaaat caaccgctac
    16561 atttattgct gctgctgctg ttctcttacc atttctagtt tgtagtatat ataatctttc
    16621 attttctata gtaaactcta tatcttgcat atctttataa tgaccttcaa gtatatgagt
    16681 aatttttaca aattcatcga atacagctgg cattacatct gcaagagttg atatattttg
    16741 aggagttctt atacctgcaa caacgtcttc accttgtgca ttcattaaaa attcaccaaa
    16801 taatttattt tctcctgtag ctggatttct agtaaatgca acacctgtac cactagtttc
    16861 tcccatatta ccaaatacca ttgattgaat atttactgct gttcctaagt tatgagctat
    16921 atcattcaat ttacggtata cgattgcacg aggattattc catgatctaa atacagcctc
    16981 tattgcaagc attaattgct cttttgggtc ttgtgggaaa tcatttttta tttctttttt
    17041 ataaattgct ttaaattctt ttactatttc ttttaaatca tcagttgtaa gttctgtatc
    17101 aactgtgtat ccctttgctt cttttactct gtctaataca ttttcaaatt tatattttgg
    17161 aacttccata gcaacatcag aaaacatttg tatgaatctt ctgtaactat catatgcaaa
    17221 tctttcattt tgtgtagcct ctgctaagcc aacaacacta ttgtcattaa gacctaggtt
    17281 aagaattgta tccatcattc ctggcataga gaatactgca ccagaacgaa ctgatactaa
    17341 taatggattc ttattgcatc ctaatgtttt atttaagtct ttttctaaag tagctaaatg
    17401 ctcttctatt tcttttatta tctctgctct tatactttca ttgttaacat aataatcatt
    17461 acatgcttct gttgttatag tgaatccagg agggacaggt aagcctatct tagtcatttc
    17521 tgctaaattg gcacctttac ctcctagtaa agatttcatg tctttgcttc cttcaccaaa
    17581 actataaaca tacttagttt ccattgttaa atcccccatt aatcttttta ttttaattta
    17641 tatcattttt attaatagac attattaaat ttctatttct ttgtatatct ttagaccatt
    17701 ttctttcatt atgtctaaaa ttatacctgc tgtttcttct attgccttat ttgaaacgtt
    17761 tattactggg catccaattc ttttcattat ctcttctgaa taatctaact cctgaagtat
    17821 tctttctaaa ttagcgtagt ttgctttact ggacaatcct aaagctttta atctttgggt
    17881 tcttatctca ttcagctttt caggtgaatt agtaagacct attatttttt tagtatctat
    17941 ctcaaacact tctttaggaa ttggtatttc tggaactaat ggaacattag ctacttttat
    18001 atttttatta gctaaataca tactcaaagg agttttagat gttcttgata ttcccactaa
    18061 aattatgtct gcttgaagaa cacctctagg gtcttttcca tcatcatatt tcactgcaaa
    18121 ttctatagct tcaactctct taaagtaaga ttcatctaat tttctaatta taccagcttc
    18181 tcttttaggt tttcttcctg ttctcttgct tatttctctt agtatatctg tcattaaatc
    18241 tatacaacta agaccttctc tttcgcaata tctttcaaca atactgcata attcttcatc
    18301 aactaatgta tatacaatta aagcgttttc agcttttgca ttctccaaag tttcaagtag
    18361 tactccttta ttaatcatgt atggaaatct ttttatttca taagtttcat tcacataaaa
    18421 ctgagacata catgcctttg caacttgttg tgcagtctcc cctactgaat ctgatactgc
    18481 atatataatt aggtttttca ccagcatacc tccttaattg tcaactattt ctaagaatag
    18541 cttagttatg ttggtttttg atattctccc aacaactctc atatgatttt tgtcttctcc
    18601 cttatattca acaatcggaa tcgaatctac ttcatgttct attatttttc ttgaagctaa
    18661 taaaacactt gctcctttag ttaaagtgac tacatttgga gttcttgtca ttatcatacc
    18721 tattggcatt ttatttatat cagaacctcc tatagttgct tttagcaagt cttttctgga
    18781 aacaactcca cataactctt cattctcatc tataataact atactcccta catcagataa
    18841 aaacatagtg actattacat catatatact ttcatccttc ttagctagta caggcatgct
    18901 cattatatcc tctactgttt tttcttttac acttttacca attaaattta tttcactaac
    18961 tcctgaataa aaatatccaa cttttggtct tgcgtctaat attcctgtca ttgttaatat
    19021 agctaaatca gatctaagtg tagcacgagt cacattcaag ctggaggcaa tactttcact
    19081 cgttattggt tcattttcct taactatatc tataattttt agttgtcttt cattaagctg
    19141 aataataatc accttctttc attgtgctat actttttatc ttttcgcatc ttatatagta
    19201 tattatataa ctatatatac cattctgtaa acagattttt tattgaatac gatatatttt
    19261 ttactaagtt ctctcgctta aagatattta ttatatatta atgggggata tttctttaat
    19321 taatagaact atattagtat tacagttaat attataatac ataattggtc attttaattc
    19381 a
//
LOCUS       DAEMGG010000072        19222 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEMGG010000072 DAEMGG010000000
VERSION     DAEMGG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.072"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            138..638
                     /locus_tag="KPK24_002348"
     CDS             138..638
                     /locus_tag="KPK24_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2300017.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSNLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(888..1667)
                     /locus_tag="KPK24_002349"
     CDS             complement(888..1667)
                     /locus_tag="KPK24_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300018.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1664..2143)
                     /locus_tag="KPK24_002350"
     CDS             complement(1664..2143)
                     /locus_tag="KPK24_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2300019.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            2642..2932
                     /locus_tag="KPK24_002351"
                     /pseudo
     CDS             2642..2932
                     /locus_tag="KPK24_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3129..4136
                     /locus_tag="KPK24_002352"
     CDS             3129..4136
                     /locus_tag="KPK24_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG2300020.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            4174..5601
                     /locus_tag="KPK24_002353"
     CDS             4174..5601
                     /locus_tag="KPK24_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG2300021.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTKKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SAGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQMYMGVEE"
     gene            5743..7008
                     /locus_tag="KPK24_002354"
     CDS             5743..7008
                     /locus_tag="KPK24_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2300022.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            7050..8648
                     /locus_tag="KPK24_002355"
     CDS             7050..8648
                     /locus_tag="KPK24_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG2300023.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            8683..9705
                     /locus_tag="KPK24_002356"
     CDS             8683..9705
                     /locus_tag="KPK24_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2300024.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(10093..11496)
                     /locus_tag="KPK24_002357"
     CDS             complement(10093..11496)
                     /locus_tag="KPK24_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2300025.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            12363..12872
                     /locus_tag="KPK24_002358"
     CDS             12363..12872
                     /locus_tag="KPK24_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG2300026.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            12900..13094
                     /gene="rpmI"
                     /locus_tag="KPK24_002359"
     CDS             12900..13094
                     /gene="rpmI"
                     /locus_tag="KPK24_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG2300027.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            13150..13506
                     /gene="rplT"
                     /locus_tag="KPK24_002360"
     CDS             13150..13506
                     /gene="rplT"
                     /locus_tag="KPK24_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG2300028.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            13816..14361
                     /locus_tag="KPK24_002361"
     CDS             13816..14361
                     /locus_tag="KPK24_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300029.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            14348..14512
                     /locus_tag="KPK24_002362"
     CDS             14348..14512
                     /locus_tag="KPK24_002362"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300030.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLLGLYFIVSFHLS
                     NDLEWYNEVF"
     gene            14801..16726
                     /locus_tag="KPK24_002363"
     CDS             14801..16726
                     /locus_tag="KPK24_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2300031.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKYTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            17127..19031
                     /locus_tag="KPK24_002364"
     CDS             17127..19031
                     /locus_tag="KPK24_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2300032.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKI
                     FGKEVNIITTSSLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         7273 a         2160 c         3391 g         6398 t
ORIGIN      
        1 agatatattg gttttaattt tatagaaata tacaaagata ttttaagcta atatatagta
       61 atttatatag cattataaaa tagaatcata taaaataagt taactacaat caaaaattaa
      121 aaaggggaaa tgttaagttg atagaaggca taaaatttat aaaggcagaa gaaaagtata
      181 tttattcgta ttggcaaact tttgataaaa tagcaaaaga gaggaaatat ctagctatgg
      241 atgaagcctt tccttttgaa gaaacagttg aatttataaa aaatattata aataaaaact
      301 taccacaact gtttataatt gatttagaat cagataactg tattggttgg tgtgacgttt
      361 tacctaaaac tgaaaaagtt ggttatttag gcatgggtat tcttaaagag tatagggaaa
      421 aaggtatcgg aagtaatctt ttaaaacaaa ttattgattt atcaaaagaa tatgggtatg
      481 aaaaaattga gcttgatgta tttaaaagta atagtagagc tattcatgtt tataaatctt
      541 tagggtttgt agaggtaaat acaatatcta gtggttttac atggaatgat agacctgtaa
      601 aagaagaagt aattcaaatg gagcttacac ttatataaaa taaagttaaa gtctttaaaa
      661 ttccatatac acttgtataa aataaagtta aagcccttaa aattccatat aaaagaaata
      721 tttttaaggg ctttttaata atgcttcttt tgaaggtagc taaatatcta aaattttata
      781 tattaaattt aaagaaaata taaagatgga agatacaacc atgtttacta ataaaaaaat
      841 taaagtcata aataataaac tcataaacaa cttattatta cttatgttta ttttgcatct
      901 attacttcat gagccatttt aactaagtca ttcatagata aatttgagtc ttgaactgta
      961 aaagaatagt aaactccatc ttgtttccat gtcaaaaact taaatttatt aacttcaact
     1021 ttgtctgatt cattatttgg ccctacacta aatgtgtatt tgcctgattt ttcatcaatt
     1081 ttatcctgtt ctgtcatttt ataaccttct ggtacaaact tttgtgcgaa tgcttcataa
     1141 gaaatattaa tatctttgta agtatctaca acttttagat tgtcagaact cttcccaagt
     1201 acattatttg ctgtgtcaag agatatttca tcattgccat ttttatattc aaaactaagt
     1261 gcttttgatt ttcctaaaac attattttct ttgtcaaatg cttttttatt tacagtatat
     1321 gctcctttaa atttatatcc attactaaat tcagaaacta tattaggggt aaagttaaag
     1381 tcttttttta cttgttctgt agttgggata gatgtatagg tagccttagt acttgatttt
     1441 ccaaccaaac tatatatatg tcctactgca agggctgttg tgctcaatat caaagtaatg
     1501 gctaaagttg caactaagcc ttttctaaat ggtaatttgt gtgttttatt attaatcatt
     1561 ttataatttt cctctctttc tggtaaacta tttagtgttt tgcttaaact attatgaaaa
     1621 ctttctggaa cttctggaaa cgaattattt aatatatttt tattcatatg attacctccg
     1681 tgttttctaa tttagttttt aataatcttc ttcctcttga taatctactt tttacagttc
     1741 cttgaggtat atctaatatt tccttaactt catctactga ataaccttca atatagtaaa
     1801 ggactattgg aattttaatt tttttaggta gactttgaat agcagtatac aaatcgctat
     1861 agtcatcttt ttcatcaatt tttttgtttt tgaagacatc ttcaaatgat agagatttta
     1921 atcgtttgcg tcttaatgaa ttacattcat ttatgactat tctaatcagc catgttttaa
     1981 agtattgttc ttccttaaga gaatccaact tttcatatgc ttttaaaata gcattgttga
     2041 cggcatcttc acagtcttgg tcgtttccta gaatagactt agatactcga tacaaagtct
     2101 gttctgattc aagtatattg tttgtaaatg tagttttatc caactaagtt ccttcccttc
     2161 tatgcaaatc gtagcgctac ttttttgtct ttcacttatt agataaaatt ttactataga
     2221 tggttcactt tttttatgat tatttgataa atttaagttt gaaagttttg cctattctat
     2281 acaaatattc tataatatat tttttctagt attttcccct catatataaa gccatgtaga
     2341 tatattaaac tttagaagcc atttatatat ttagttttat agtaagtctt tatattgagt
     2401 aattagaaaa tagtaagtat ttaatgttga gtagttagaa taacaatgct tttggattat
     2461 ttacttcatg tacagattta aaataagttc tttaaatagc aactttttga aaaaacgcaa
     2521 aaatatttta tgttgtctga ttatatttat agatgtgaaa caatattgta tagagtaaaa
     2581 gtatatagaa gatgattaca tagagtttat acttgaattt tacaaatata attatcaaaa
     2641 tactgtcaaa gagaaaaata gttttgatga acgtttttat caaattgcta cagagacagg
     2701 gctaagtgct aatactgtat ataaagtatt caataaaata ataaatagtg gaaaaactga
     2761 atttacatca aaggaattag ctactatatg tggtgtaagt attagaacta tggacaggat
     2821 tatacttaga ttgtgtgatt ctggttattg tgaggtagtg agtgaacagc ttatgagcaa
     2881 gtatggtaga cccagtagaa tattacgttt taaacctttt ctgttgtttt aaatagatat
     2941 aagtcaaaaa ttaaattttt atgataacat tttctttaca attttttaga tatatgttaa
     3001 tatttagaaa aatgcatatg tattcataaa ataaacttaa aacagctaaa aaatcattaa
     3061 aaaggaaatc gtatgcatga aatgagaaag ttaatttata acttaaataa atctgggagg
     3121 agataattat gggaaaatat caggattcaa aaaaagttgt aagaaattat tttgaagcat
     3181 tagaaaatgc tactggggat gaggttgaaa atgtcttaaa gcaacatatg aaatcagatt
     3241 ataactggaa aggtgtgtat ccatttcgtg aacaagaagg cactgaaaat gtagcagatg
     3301 tattttggaa gcctttaaaa aaatcattga gtaatatgca aagacgacaa gatattttca
     3361 ttgcaggaac aaatgagatt gacaatagtg aatgggtaat gagtatgggg cattttatgg
     3421 gactttttga taacgattgg cttggcatta aacatactcg taaaatgatt agtttaagat
     3481 atgcagaatt taactgtgtt caagatggta aaattgctaa aactggatta tttgtagata
     3541 taattgggtt tatgattcaa gcaggagtta atccattgcc accacaaaca ggttcatatt
     3601 ttgtatatcc agggccaatt gaccataatg gattactttt tgaagatgca gatgaagttg
     3661 aaggagtaaa aactttagca ttagtaaata aaatggtaga tgatttatct gaattaaatg
     3721 aaagtggtgc tatgggatgc ccaccagaaa tacttgaaaa atcttgggct aaagatatga
     3781 tttggtatgg tccaggtgga attggtgctt cttatactat acctagatat caagagcaac
     3841 accagctacc atttagaaat aatttaaaag gaaagacttt caatgggcat gtatgtagat
     3901 ttgcagaagg taattttgct tgtttctttg gatggcctaa tctgactaat gtaccatgtg
     3961 gaggtttttt aggattacca ggtggagagg ttaaagcaga tatgcaagtt gttgatgttt
     4021 attatcgtaa gggcgataaa cttcaagaaa actgggtgtt aattgatatt ccttattggt
     4081 tgaaacaaca agggcttgat attcttgaga gaactaaaag tatttttaat gcttagttat
     4141 gcatacgtat tctaaaataa agggggatat aaagtggcat ctaccaacaa aaataatgca
     4201 acaagtaaag aagaagaaaa agaagtttct acaagtgggc ttgaatcatg tacggttaaa
     4261 gaaaacaaag ctaaaatgaa tcaaactaat tttagtaata taaatgaaaa tcaaacaaat
     4321 gagaataaaa cgaacacata taaaagaaat atagataaag gaaatataga taaagggaat
     4381 atagataaaa caaacacaaa taaatccgac ataaataaag ttaacataga tacagtggat
     4441 acagataaat taaatacaaa tactcttact aaaaaaaata aaaaagttat atttaagggg
     4501 gaagataata tgtcatctac aaaaatttac tatggagatt cattagaagt aacgcctgtt
     4561 ggagtaatgg attacaatga tttttcaaaa cagacaaaaa gggaacagga aatacctgga
     4621 tttgattcta agtatagaga ttttgtagat tatattatga agattacaca taatatctgg
     4681 gaagaaaaag gtataggtgt tatttatgat acatatcaca ataatgttac aatgcattgt
     4741 ggctcttcaa atttagttgg tattaaagat gttatttcca atactcttca aacattacat
     4801 gcgtttcctg atagaagatt aattggtcaa aatgtaatat ggtctaactt tggtgctgat
     4861 ggttttttat cttctcaccg tgttttgtct acagcaacta acttaggtga tagtaatttt
     4921 ggaccagcaa caggtagaaa aataaacttt agaactgtaa ttgattgtgc cacaactaac
     4981 aatagaatcc acgaagaatg gttggttaga gataatttat ggattgttac tcaattagga
     5041 ttaaaccctc aggaagtagc aaagagtatg gcaaaggcaa gtgaatctaa agtgcttagt
     5101 ttacaatcaa cttatggtat ctgtgaatct atggatggtc aatttatgcc aactaagtat
     5161 caagcaaaag atgattctgc tggcgaaatg atgcttgaaa tgacaagccg tatttataat
     5221 tataaatata taaatgaagt taaaaaatac tatcatgata atgcagtagt acattttata
     5281 tgtgataaag atttaaatgg ttatgatgag attcaaggaa tgattgtaag tttacttgca
     5341 tcaattccaa atggaagtta tgaagtggaa agagtaactt gtaatcaaag agaaaaaaat
     5401 gaaggttatg atgtatctgt aagatggaga ttacgtggaa taaatgaagg tataggattt
     5461 tttggacagc cttctggaaa gcatatagaa gtaatgggaa tcaatcatta tcatatacta
     5521 caaggtaaag taaaggagga gtggataact tttgatggta tggatgtatt aaaacaaatg
     5581 tatatgggag tagaagaata aagctgttta gagtataaat agttttgaaa tatataggga
     5641 agtattgctt tataaaattt aagtggggaa tgtatatgaa aaatagatgt ttaaactttt
     5701 agcaaaatta tgactaaatt taaaaggggg aattataaaa atatgaaaac aacggttgaa
     5761 atagaaaatg atatcatatt aggaaaagac tattcaaacc taaaaagagg aattgcattt
     5821 ttttcaattg ctttaatata ctttttttat tgctacaatt ttatggtggg tacttttatt
     5881 aaaccaacaa tgatttatgc tttagcagat gggggatttg gtttttcatt aaaacaaaca
     5941 gaagaaatat ttgcagttat gtcttttggt acaattccag gtacatttat ttttggggtg
     6001 atttctacaa aaattgggaa gaagaggact ttaattagcg ttgctttact gattggattg
     6061 acaacattta taccaatgtt atctcctaca aatattatgt tatggaaaat agcacgtttg
     6121 tgtacaggag tagtgttggg aggcgtattt ggaacagcta tgcctttagt tgcagatatg
     6181 tttccaagta aatatcgtgg taagttagct gctatattaa ctgctttatt ttcattagca
     6241 atgatttttg gtgggaaagt atatggcgtt ttaggtgatg caaattggca aatattgatg
     6301 tatactgcga ttataccacc tatagttgga gctatcttag caattttctt tgtgccagat
     6361 gatttggagt atacaaagga attagttaaa aagggaaaag aaactggtga aaagattagc
     6421 tatataagta tgtataaggg aaaatactta tggattggat tgggaacgat tcttttatca
     6481 ggtgcaaatt ttacagcata ttcagcattt tcaaataatg caactacata cctagtgaca
     6541 ggaataggaa tgtcagcagc agtggctggg tctatatata gtttacaagg aattggtcaa
     6601 ttagtaggat atttattctg gggttcaatt gcagataaat ttggaagaaa aatacctttt
     6661 ataggtatgg cattagcagc agtatttgtt tttatatata caaaattagg tggagataat
     6721 acaacaacat tctatatgat atcagtatta ctaggtgttg ctgttggata ttctggagca
     6781 tggggagcat actacacaga gttgttccca agtaaatatc gttcgttgtc atctggtatg
     6841 tcatttaatg gaggaaggat tatatctacg tttgcaatac ctgctatagc tggtacagct
     6901 tctggtactt taggtatgat gactatcttt tacatatcaa tcgcagtctt tgtagcaggt
     6961 gctattgtat ggttattctt accggagacg ttaaataata aaaaataaaa tactaggaat
     7021 ttattttgtt acttagaaag gggtggctta tgatttcatc aattgatgta tgtattataa
     7081 taggatattt gctattaatg ttagttattg gttattattc aggaaaagat aataaaaatc
     7141 aagaagatta tttcttggca ggtaggtcaa tgccatggat tccaattgct ttatcagttg
     7201 cagctactat gattagtgca aatggcttta taggtggtcc tggatgggca tatgtagatg
     7261 gaatgtatcc agtaatggtt aatattacag tacctttagc aatatttttt gcactgagca
     7321 ttaccacccc agttatctat aaattaaaaa taacctctat atatgaatat atgggacttc
     7381 gtttaggaaa ttattcaaga ggattaacta ttattcaatt ttttattaat tcattgattc
     7441 aagctagttc tatggtatat attccagtgc ttattattca aatgattact ggctggtcac
     7501 tttatgcttt agtcccaata gttgtacttg tatcaattat atatactcta ttaggtggga
     7561 ttaaggcagt tatatggact gatagtattc aaatgatagt agtaataagt gctgtattta
     7621 tagtaattat tgtagcctta aaaggaatga atctaagttt ctttgataca ttacaaattg
     7681 cacaacagag tggaaaatta aatacatttg atttcagtag ggatatttca gtgacaaata
     7741 ctttttgggc tactttaatt ggtggaactg taatgtggat aagatacttt tgttttgacc
     7801 aatctcaagt acaaagagtt ttaacatcta aatctttaaa atgtgcaaaa aattcttttg
     7861 tagttagtgc atttgttatg aatttagttt actactttat gctattggta ggagtaattt
     7921 tatttgtttt ttatggggga aggacatttg aaacttcaaa tgaaataatg attactttta
     7981 ttttaaatga acttccagta ggagccattg gaattattat tgcaggtgtg tttgcagcag
     8041 caatgtctag tatcgactct atattaaaca gtatgacaac tgtttttacg aaggatattt
     8101 atgagtttta tttcaaaaaa gacaacaaag aatcatcatt aaaagttaca atgattataa
     8161 ctgtagttat aggagttgtt atgacaggtt ttattattat gggatttgga ggaagcatca
     8221 aatcaattct tgaccttgtt ggtaactata tctcatactt tgctggacca gctacaggtg
     8281 catttgtact agcaatgttt acatataaag ctaatgataa aggagtatct attggtttta
     8341 ttgtagggtt agttttagga ttttttattt ctaataaata taatgtgagt tggttgtgga
     8401 atccagcaat aggtgctaca ataacattga tatttggata tatttttagt gtcattttaa
     8461 agaaaaatca taatgcagat gatattaaaa aatacactgc atttggtatg agaaatcacc
     8521 ttatttctca aggaatggat aaagtagatg gagtatctat agtgccattt tctttaggta
     8581 aacaagaact aattgtatta gcttttttta ttatccaata tattatatta tttttgatac
     8641 aattttaaga agaaatacta gatatttaag gagaaatact aaatgaatca aaagaaagtt
     8701 cattataata aatcggctac ctcaaaagat gttgcaagat tggcaggagt atctcaatca
     8761 tctgtttcaa gagcatttgg tagtgcaaat ggaaagggcg taaaacctga agtaagagaa
     8821 agaatttttc gtgtagcaaa cgaaatagga tatgttccta atttggtagc acggggaatg
     8881 attagtggaa aaactaatgt tataggattg atagttggtg atagtctagg tccattttac
     8941 aatagaatta tcaatttatt tgtagaaaaa attcaagaga taggtaaaca gtgtctcgtt
     9001 tttaaagttc ctagacaaca aagtatagat agtataattt caaaagtaat tcagtttcaa
     9061 gtagaagctg tgattattac agcttcggcc atgaataagg tgatggctga gacttgtgaa
     9121 gaaaacaata ttcctgtgat tctttttaat cgttttatac ctggtataaa aataagtaca
     9181 gtctatgtag acccaattga aggtggggga atggctgctg aatatctgct taagaaaggt
     9241 cataagaata taggatatat tcaatttacg agagaaacaa gtgaagaaat ggaaaagaaa
     9301 attggatttt attctaagtt aagacaaagt gggattcata accttaaaga agaatctgca
     9361 aactatgatt atgattctgg atatgaagca ggtaagagaa tgttagcatt atcaaatcct
     9421 ccaacagcaa ttttttgcac aagtgacttg atagctattg gtgtaatgga tgctgcaagg
     9481 tttgagtaca agttgagggt gccagaggat ttatcagtga taggatatga tgatattcaa
     9541 atggcttcat ggaagagtta taatttaacg acgattagac agccactaga tttgttgatt
     9601 gaaaaaacaa ttaacatact taagtcctta ttaaatgaag aaaatccaga gtcagttgtt
     9661 gaaatgttaa aacctgaatt gattgaaaga ggttctacac tttaagtggt agatatttaa
     9721 ttaagtggta gatacctaat taagttgtag atatctgata agaaattttt aagttttcaa
     9781 tcgttctaaa ttgtgagtat aaaaatctac taaatttagt tatgtaaaat ataaattata
     9841 agtgtagaaa ctaggctact agcttttata ccacttcaaa cattactgga ggtagttaag
     9901 tttacatcag tttagtttta tgtagatata atattctgta aatagacaaa attaaaatat
     9961 gtaagtgtct caaaatgaaa tttttagttt atgaggcact tttttgtata aaatcaaatg
    10021 tatttttata actttaacaa taaaaagagg cttatctcta ttttgagaca gcctcaaatt
    10081 tttaaaacta tattagttta cagcttcttt tcctatcaaa tcttttaact cttcatgagt
    10141 catagtttct ttttcaaata agaatttaga aacagaatgt aaatcttcaa gattatcctt
    10201 taacatctct aaagtctctt tataacaaat ttcaactata cgatttgctt ccttttgaac
    10261 cttatcacat aagaaagtct tatcatttcc atcaatagtc ataaatccta agttactcat
    10321 accatattca caaaccattt ggttagctat ttcagtgact ttctttaagt cgtcctttgc
    10381 accagtagat ttatgattaa atattaattc ttctgaagcc tttccagcaa gtaatatttt
    10441 tatttttcca atcaactcat ccttagtata aatatatcta tcttcatcag gtaattgaag
    10501 aacataacct aatgcttgtc ctctaggaac tatagatatt ttttgtatag gacaaattcc
    10561 tactatatca cttacaagag catgtccagc ttcatgatat gctactattt ttttctcttt
    10621 ttcaaccaat gcagagtttt tagattctag ccctgcaata actctttcaa gtgccttgtc
    10681 aaaatgttct gatgtaatac actcactgtc atctctaact gcaaatatag ctgcttcatt
    10741 tgctatattt gaaagatgag ccccattgaa accatgagtt tttttagcta aaagttctag
    10801 atttacagat ttgtctatag gtttgtcatc tgtatgtacc tttagaattt caaatctagt
    10861 gtgatagttt ggtgcaccta tatgaatgtg tctgtcaaat cttcctggtc taagtaaagc
    10921 ttcatctaat aagtcaagtc tattggtagc acctattatt aatacattgg aatctttatt
    10981 aaatccatcc atttcaacta aaagttggtt tagtgtttgg tctttttcgt tgttactttc
    11041 aagatgtctc tttgctccta cagcatctat ttcgtctata aatattatac ttggagcttc
    11101 ttttctagct ttttcaaata gagttctaac tctttttgct cccacaccta catatttttc
    11161 aacgaactct gaaccagtaa cattgaaaaa tgatgagttt gtttcaccag ctacagcaga
    11221 tgcaagtagg gttttacctg ttcctggagg accataaaat aatactcctt taggtatttt
    11281 tgcacccatt ttttgatatt tttgtggtga cttcataaaa tcgactattt caaatagttc
    11341 ttcttttact tcatctaatc cagcaacatc tctaaatgta gtctttggtt ttgtagaaag
    11401 tgaatcatct gtttgattat ctctttccat ttttgtctgt gcaaagacag gagctggatt
    11461 gttgattttc ttaaaaaagt tgtttagcat tttcatattc acacctcagg taattaatta
    11521 ttgttaatca attttatatt ttccatattt actatttttt attatttatt tttcatggaa
    11581 aaaatataca tgttatctaa ataaaaataa atttgtaaga tgtatgaaaa aatgaattta
    11641 tgtatataga gatttctact tatgtattga ataaagtatt acaaattata aattatgtat
    11701 tgattatata ttgagtaaag tattataaag catgtattga ttaagtattg aataaaatat
    11761 tacaaattta gttgaaatga aaatgataaa aaatcttgta ctatatttta caaatcatat
    11821 aaatagagaa attgttgtag cacaaagtag taggtagtat attagaatat aatataggat
    11881 ttgttgtgta gtatagtagc atattagaat ataatatagg atttgttatg tagtatagta
    11941 gtatattaga atacaatata gaatttgttg ggtagtatag tgttatatta gaatacaata
    12001 taggatttgt tatgtagaat atatagagtc atgtagaata taaatatata aaaggatttt
    12061 gggtataaat ttaaaaatta atagcaaaat gagtagtata gacatataat tagtagtagg
    12121 aagtaattaa atttttccta aacaaaaaaa atagttgcaa ataatttgat taagtgatat
    12181 agtagataag gtaagaataa aacaaagaag aagtccgctt ctcaccttac gccggaggtt
    12241 agtaaggtaa atataagtta aagcttaatt ttaatttaag tttgtttaaa tttggtaact
    12301 tatattgcgg atgattacgt catccgcttt ttattttata aaaattttgg aggtgtccta
    12361 caattagcaa agaattagca atcaatgaac aaataaaaga taaggaaata agagtccttt
    12421 caccaacagg tgaacaactt ggagtaatgc ctactaaaga agctcaagca atggctaata
    12481 gcaaaaactt agatttggtt caaatctcgc cgaatgcaaa tccaccagta tgtaagataa
    12541 tggattatgg aaagtttaga tatgagcaag cgagaaaaga gaaagaagct aagaaaaaac
    12601 aaaaaactat agttgtaaaa gaagtaagac ttagaccagg tattgaacaa aatgatttaa
    12661 atacgaaagc taataatgct ataaagttcc ttaaaaaagg ggataaagtc aaagttgaac
    12721 ttagatttag aggaagagaa ttaggacata aagacatagg taaagaagtt atgcttaagt
    12781 ttttagatat aataaaagag tttggagaac caacaaaagc ccctgcattt gagggtaata
    12841 atatggttgt aattatagat ccaaaaaaat agatgattaa ctgagaggag gaatttgtca
    12901 tgcctaaaat gaaaactcat agaggtgcag caaagagatt aaagaaaaca ggaactggaa
    12961 agttaaagag agctaaagct tttaaaaaac atatattaac taaaaaatct gcaaaaacta
    13021 aaatgaactt aagaaaatca actttagtaa gtgatggaga tgctaagaga atagcacaat
    13081 tattaccata ctaagataca gattgtaaat aataactata gaagagggat ataaaaggag
    13141 gtcataacga tggcaagagt taaaaaagct atgaacgctc gtaaaaagca taagaaaata
    13201 ttaaaacttg caaaaggatt cagaggatct agaagcaaat tatatagacc agcaaataca
    13261 ttcgtaatga aagcattaaa aaatgcttat ataggaagaa agttaaagaa gagagacttc
    13321 agaaaacttt ggatacaaag aataaatgca gctgctagaa tgaatggaat atcttattca
    13381 agattaatga atggattaaa attatctggt attgaagtta acagaaaaat gttatctgaa
    13441 atggctatac aagacccaga aggatttgca aaattagcag aagttgctaa agcaaaatta
    13501 gcttagtatt gaatcatgag atgtaaagtc tttgagttat ataaataatt caaggacttt
    13561 tttatttttt gaaaaatact tattagtgtc atgtacaacc aattttataa tgataaataa
    13621 aataagcaat acaatagaat tttatgagat ttattataag aattgttaca attatttatg
    13681 atagctataa aaagtcatag taaaaatgat attgatgtat gttgatatat gtataaagct
    13741 ttcaatcaat cttgaattaa tattatcaac atgataatat tatcttatta ataatataaa
    13801 taagggggag ataatatgaa tgaaaaacta atggattgtt ttgtaaaccc aattaaatgc
    13861 aagttattga ttgaaatttt ttcaaataat cgaactacag ctaaacaatt aggagaaaaa
    13921 tttcaagata tatcaaaggc aacactttat cgttacctta ataaaatgtt gaaagatgat
    13981 attataaaag tagttgaaga aaataaaata aggggaactg ttgaaaaagt atatgctatg
    14041 aagttagata tagctgatga tgtgaacaaa attatagatg agaactctgg agaagaatat
    14101 gctcaactgt ttactcaata tatgatggga tttttaagag aattttatga atacgctgaa
    14161 agaaaggaaa aggatatcct aaaagatggt tcaggatttt ctgtctgtcc agtatatgct
    14221 agtattgatg agatagttga agtgagtaaa aaaattgcta gtatactaga acctctaaga
    14281 aataactcgc acgaagaagg cagaagaatg cattctatag gagtgataat cactccacct
    14341 aaagaggatg aagggaaata aatctgattt acatgaggta gagccaattt acacggaaaa
    14401 tcgttttatg atttacatat gtatccagag caaagtatct ctgcttggat tgtacttcat
    14461 tgtatcattt catctatcta atgacttgga gtggtacaat gaagtttttt aatttttcat
    14521 atcaatgcct aaagaaatga actttagacc acatatgcaa ttaggttaca ttatacaatt
    14581 agaccagata tacaaaataa aaatcagtat ttttatagca gataaattga ctgttattct
    14641 tcttaaaaat catctattag ttattgttag catattgaaa gaaattatag gaatctattg
    14701 aataaatgaa tattgcccat aatcatataa actgctatag tttaaaataa agcaggacgt
    14761 taaattttat agtaatctaa aaatttaaga gggggatttt atgaaaataa aatttgttat
    14821 tatgccattg gtgatatttt taatggtatt atttgtgggg tgtcaaagta caaaagatac
    14881 agaagttaaa gcaaaatata cagaaattaa aatattaggt acaggtgatt tgcattcaat
    14941 tttgacagac tctatggttt catatgtaaa tgaggaaagg gaaaagaata aaaatctgct
    15001 tatggtagat gcgggagatt tctatggtac acaaagtaga gaaatgtggg aatggtcttc
    15061 tggaaaaaag ttaattaata ttaaaagaaa tggtagagca gagtatgatg acataattaa
    15121 gagtagtaaa gatgaagtac ctatagttaa agatatggca aagttgaaat atgatgctgt
    15181 agttttggga gataatgaat ttgtttcaaa tgataagcaa agtcttgata aactagtttc
    15241 tgattttaaa aataataata tgccattggt atcagcaaat atatatgaac aatctggaga
    15301 aaattatgta caaccatatg taatgaaaaa tattaagaca gatgagggca atgtaaaagt
    15361 tggaatacta ggtcttacta taaaagaagt aggggaaacc cttggtcttg aggattttga
    15421 aaaggataaa aaagctagag agcttggaga acaagctgat tacaaaggaa aattatatgc
    15481 aaatgattta gtagaagatg caaagaaatg gataaaagtt atggaaaaag aaagtccaga
    15541 cataatagtt gcagttgtac actcagggga agaacctaaa acagatagaa atccaggaaa
    15601 tagaataaaa gagttagcaa ctacagtgga tggaatagat gcaattgtag ctggtcatac
    15661 tcatgaaaaa atagatgaac acaaatataa aaataaatct ggagatgaag taattgttac
    15721 acaaccaggt tggcatggag atagagtatc agagattaat ttcaaattaa ataaagagaa
    15781 tggaaagtgg gacataaagg aaaagtctag taaattaaaa ataattgata aagaagtaag
    15841 tatagttgct actgcagatt tacattcaca tttctctgat aaattaacag ttgaccttgt
    15901 taatgaacgt agtaacccaa ttgaacctgt tgtagtagat gcaggcgact tcttagaccc
    15961 tcaaacagat gaaatgactc aatggtataa agagtggaaa agtataagag ataataatag
    16021 tgataaaacg attagtagat gcccaatggt catggatatg aatcagggaa tgtatgatgc
    16081 agtagtactt ggaaatcatg aatttgtatc cgaaaaagat gaattttggt cagatgaacg
    16141 ccttttagat gctgttatag aggattttga acaggtggca atttctgtgt tatcggcaaa
    16201 tatatatagg caatctggta aaaattatgt taaaccatat attataaaag atgtggagac
    16261 gagtgaaggt aatgtaaaag taggaatatt gggacttact ataaaagaag taggtaaagg
    16321 tcttaaaaat gtaaacttac aagagcaatt tcaatataag gataaattgt atgcaaatga
    16381 tttagtggaa gatgcaaaga aatgggtaaa agttatgaaa aagaaaaaac cagatataat
    16441 agttgcagtt gtacattcag gagaagaacc aaaaaatcca aaacatccag gaaatagaat
    16501 aaaagaacta gcaactacag tagatggaat agatgcaatt gttgctagtc atactcatga
    16561 aaaaatagat gagcatgaat ataaaaataa gtctggagaa aaagttatag tgacacaacc
    16621 aggtgagcat ggtaaatatt attctaaaat tacctttaaa gtaaataaag aaaagggttc
    16681 gtggattatt aatgataaat tcagtaaaac aattgaggtt gaataacttt ataaaattta
    16741 tagataaagt aaattatctg atattgacat gatatataag ataaagtttc taatattagt
    16801 ataatgcata atgtgaaata ttttaataat aagagaataa acaagacaaa taaaaatatt
    16861 ttaacatgta agatatagta tgaactatta tactaatgct tactttataa tttgaacatg
    16921 ttagaataag tcttatttga aaattttagt gtattagagt aagtattatt ttaccagtaa
    16981 aaaatattga gaactatatg tagatttgct taaaaaggaa tttacatata gttcttttcg
    17041 tataataaat agtacaatct tatatggtaa agataaagtg tgatattaaa ttttattatc
    17101 atccaaaatc taaggaggag tctcttatga aaataaaatt tattatagta gcatcaataa
    17161 tatttttaat agtattactt gtggggtgtc aaagtactaa agacacagaa atcaatgtac
    17221 tcgccacaag tgatttacat tcaattattc cagataattt aatttcctat gtagaagagg
    17281 agagaaaata tgatgagaat ctacttatgg tagatgcagg agattttttt gacatgcaaa
    17341 gtggtgagat gaataagtgg tttactggtc aaaaattagt aagatttaaa gatgggatac
    17401 cagagtacaa aaaaatagct gaaccacgtg aaggagaagt tccattggct aaaaaaatgg
    17461 caaagctgaa gtatgatgca gttacatttg ggaatcatga atttgtatct aatgacaagc
    17521 agagccttga taaattggtt tctgatttta aaaataacaa tataccatta gtatcagcaa
    17581 acgtatatga acaatctgga gaaaactacg taaagccata tgtaatgaaa aatataaata
    17641 ctgacaaggg aactgtaaag gttggtatac ttggtctgac tataaaagaa gtaggggaac
    17701 gttcaaggtt taaaagtttt gaacaagata aaaaggctag agaacttggg gaacaacctc
    17761 aatacaaagg gaaattatat gcaaatgatt tagtagaaga tgcaaataag tgggtaaagg
    17821 ttatggaaaa aaaagaaaag ccagatataa tagttgccat tgtacactca ggtgaagagc
    17881 caaaaaatcc taaaaatcca ggaaatagaa taaaagaact agcaacaact gtagagggaa
    17941 tagatgctat tgtagcaggt catacacatg aaaaaataga acagcataca tataaaaata
    18001 attctggtga agaagtaata gtaacacagc ctggagctca tggtaatagt atatcaaaga
    18061 ttaattttaa gttggataag aagaatgata agtggtttat aaaagacaaa tatagtgaat
    18121 taaagatttt tggaaaggaa gtaaatataa ttacaacttc tagtctgaat ccaaaatttt
    18181 cagatgaaat ggcagttaat ttgtttaatg aaagagatag agatgttcaa cctatattta
    18241 tagatactgg agatttttta aatactaata caaaagaaat gactaagtgg gctaaagagt
    18301 ggcaatacaa tgaaaataaa ggcatctata aaaaaataaa tgaatggcca attgtatatc
    18361 ttggttatga tgctgtagta cttggtagcc atgaatttac agaggataaa gagacttttg
    18421 aggaaaaaaa atctacattg gattatatta tagaaaactt tgaagaaatg aagataccag
    18481 tattatcagc taatatatat gatgaatctg gagaaaatta tgtaaaacct tatataattc
    18541 ataatgtaga aactagggaa ggaaatataa aggttggagt attaggtctt actactactg
    18601 ataaggatga agaagactta agtagctcaa gcttaagcaa agactcaaag acttcgaaaa
    18661 ttcctgaaga aacaggaaag ttatatacta ataatctagt aaaagatgca agcgaatggg
    18721 taaaagttat gcaaaaggaa agtcctgatg tgattgttgc agttgtacat tcagataata
    18781 atagtactct aaaaaatcca agtagtgaag tcaaaaaact tgcaatgtca gtagatggaa
    18841 tagatgctat tgttgtaggt catacgaaaa ataaggtaga agaacatatt tacaaaaata
    18901 agtctgggga tgaggttata gtaacacaat ctggagaaga ttgttattca aaaataagtc
    18961 ttgaactaag aaaagaaaat ggcaagtgga atatcataaa caaatatagt aaagcaataa
    19021 aaatgaagtg aatttattga tatataaacc attattaaca ctgattttta taatataata
    19081 aatactgatt ctataatata attaacacta ctttttataa tgtcatcaac actgattcta
    19141 taatataatt aatactactt ttttaaatat aatcaatact gattccataa tataattaac
    19201 actacttttt taaatataat ca
//
LOCUS       DAEMGG010000073        18767 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEMGG010000073 DAEMGG010000000
VERSION     DAEMGG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.073"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            327..1757
                     /locus_tag="KPK24_002365"
     CDS             327..1757
                     /locus_tag="KPK24_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2300033.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1860..2963
                     /locus_tag="KPK24_002366"
     CDS             1860..2963
                     /locus_tag="KPK24_002366"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG2300034.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2978..3214
                     /locus_tag="KPK24_002367"
     CDS             2978..3214
                     /locus_tag="KPK24_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2300035.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3231..4280
                     /gene="vorB"
                     /locus_tag="KPK24_002368"
     CDS             3231..4280
                     /gene="vorB"
                     /locus_tag="KPK24_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG2300036.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALTSKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4282..5022
                     /locus_tag="KPK24_002369"
     CDS             4282..5022
                     /locus_tag="KPK24_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG2300037.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5025..5564
                     /locus_tag="KPK24_002370"
     CDS             5025..5564
                     /locus_tag="KPK24_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG2300038.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5688..7787
                     /locus_tag="KPK24_002371"
     CDS             5688..7787
                     /locus_tag="KPK24_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG2300039.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIENRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8054..8569)
                     /locus_tag="KPK24_002372"
     CDS             complement(8054..8569)
                     /locus_tag="KPK24_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG2300040.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8661..9872)
                     /locus_tag="KPK24_002373"
     CDS             complement(8661..9872)
                     /locus_tag="KPK24_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG2300041.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10548..11990
                     /locus_tag="KPK24_002374"
     CDS             10548..11990
                     /locus_tag="KPK24_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG2300042.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            12032..12385
                     /locus_tag="KPK24_002375"
     CDS             12032..12385
                     /locus_tag="KPK24_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300043.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12576..13397
                     /locus_tag="KPK24_002376"
     CDS             12576..13397
                     /locus_tag="KPK24_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG2300044.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            13655..15538
                     /locus_tag="KPK24_002377"
     CDS             13655..15538
                     /locus_tag="KPK24_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG2300045.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            15763..16443
                     /gene="pgmB"
                     /locus_tag="KPK24_002378"
     CDS             15763..16443
                     /gene="pgmB"
                     /locus_tag="KPK24_002378"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG2300046.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            16440..18725
                     /locus_tag="KPK24_002379"
     CDS             16440..18725
                     /locus_tag="KPK24_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG2300047.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKIIVYGKKYILEKCIKIDK"
BASE COUNT         7332 a         2038 c         3407 g         5990 t
ORIGIN      
        1 tcttgaataa aattataaaa aattaaaatc actagaaata tgataaaata atttaaaata
       61 cgcatttcaa tgacatttca aaacaataaa ataataaaaa acaaacctaa attaagtcta
      121 aatagctttt tagttaattt tagtagtaaa aaataaaact taaaaagtgt ttgacaatta
      181 tgaattttga gaatataatt aaattaaaat tatacacata taaaaatata ctcctgaatc
      241 aagaatgaga tatttaagat tcaaaatata tattattata cgtgaataat aaaaaagtaa
      301 gaaaatttta taagggaggg gtagatgtga gtagtcaaga aagcaaacca aaaggaaatc
      361 ttatatcaaa ttttagagat gatatgagag accctaagaa aagagcaata tacacatatt
      421 caagttctat aaacttagcg tacaatttta taggttactt tgtatcaact gttacaaata
      481 tttttttaac tgatgtggca aaacttggtg taattatggc aggtggtatg caaattatac
      541 aatctttcat aaaaattgct gtagctccta taacaggtac agtatttgac aaacaaccat
      601 tcaaaaaagg aaagtattat ccatggctta gatatgcacc agcagcgtta gcattaacat
      661 atggaatatt tttcatagtt ccattaatga aaataagtcc agcattactt ttacctttga
      721 tgacagcttt attattaata gcatcttgtg ctcagcaagt agttttcact ataatttatg
      781 ctatttaccc tgtagttgct aagacaccta gagaccgagt tataggctca actactacga
      841 atatatttaa agaagttggt aaattcttag taggtttttc atatccgtta ttattagttt
      901 tatttgcaga tgtattaggt ggagaaggaa tgggttattt aggtacctac ttattctttg
      961 cagcaggatg ccttttagta ttttggtttt cttcaacaga gataataaaa tctggagcag
     1021 aagaagaagt tcttcaaaaa gaagagaagc caaagattaa agcaagtgaa atggcaaaag
     1081 ctttattcac taatgtacca cttatgatgg cattttgttt agaatttctt atatgtataa
     1141 gatctatagg attaggacct ttggctccat attattttaa atatgtaata gaagatgaac
     1201 gtggattagc agtattttta tcaatcatgc ctttggtaag tgtagcattc atgtttttcg
     1261 caccagtgtt tataaaaatc tgtagagagc aaaaactagc ttcaataatt tctttttcta
     1321 tatgtgcact atgccactta ttggttgcat ggactccttg ggggaaaact actttaggag
     1381 taactatgtt gctggcaatt ggtggaggtt tttcaaatgt agtatcaata ataaatctta
     1441 atttctttgc cggttcttgt gattatgggc attggaaatc aggaaaagac ttacctggct
     1501 taagtatgtc actatatcca gttgcaatac aagtaggagt attacttgca actactataa
     1561 gaacagtttt aatgaactca atgggatatc aagctgatat ggtagtcaca gaagcagtta
     1621 aatcaggttt tataaatatg atttcttatt ctatggcaat acctttaata atagctgttg
     1681 ttatagcaat actatatcca gtaagtgaca agaaattaaa ccaaataaga gaagagttaa
     1741 atcaaagaaa tgcttagtta aaagaaagtt aagtagatgg ttaatttaag ctattaaagt
     1801 aagtagacgc aatagcgttt atataaatga aaatacatat acagaggggg cttttaaaaa
     1861 tgatagaaaa attattgaga aataccgtaa aagaattaca tcaatatgtt ccaggtgaac
     1921 caatagaaaa agttaaggaa aaatatggtg taaaagaaat aataaaatta gcttcaaatg
     1981 aaaatcctct aggaccatca ccaaaagcaa tagaagctat gattgaaatg ttaaaacaag
     2041 gtcaacttta tccagagcca gaagctaacg aacttagaag aaaattagct gaaaagctag
     2101 atttaaaacc agaaaatttt attgttgcta atggtgcaga taacgttatc actctaatag
     2161 gtgaggcttt tataaataga ggagatgaag ttatatattg taacccaaca tttccatcat
     2221 atagaactgc aactataaaa aatgaaggga taccagtaga agtaccttta acagaagatt
     2281 ataagtatga tttacaaggt atattagata agattacaga taaaacaaaa ttaatatgtg
     2341 tatgtaaccc taataatcca acaggaacaa ttgtggatga taaagagtta gaagaatttt
     2401 taaagaaagt ccctgaaaat attataacta tattggatga agcatatata gaatttttaa
     2461 cagttcctaa ttatgtggat ggacttaagt atgtaaggga aaattataac gttatagtaa
     2521 caagaacttt ctctaaaata tatggtcttg cagggcttag agtaggatat ggtattgcta
     2581 aagatgagat aataagaact ttatttacag taaaagagcc tttctctgca aatcgtgttg
     2641 ctataagtgg agcaacagca gcacttgatg atgaagagtt tataaaagaa acatatgaat
     2701 taaatagagt tggaatggct tactttaaag aagagtttac aaaaatggga tttgatgttg
     2761 tagattcaca atcaaatttc ctttatgtag atatgaaaac agatatacct aagttatttg
     2821 aagacttgaa aaaaagaggg tttgtaataa gaccaagtgc tacacatgca cgtgtaagta
     2881 ttggaactat ggaagaaaat aagaaatttg tagcagtttt aaaggaaata ttaaatatta
     2941 aaggagagcc aagtattgga taatagatag aaatattatg aaacggggtg tagatatgaa
     3001 cttaaaaata aacgcagaga gatgtaaaag ttgtgaatac tgcgttatat cttgtaaaaa
     3061 aggagcatta aaaatatcaa gtaagataaa caaagaaggt tatgctcatg tagaagttga
     3121 tgagagtaaa tgcatacttt gtggaatttg ctatcaggtg tgtccagata atgtttttga
     3181 aataattaaa gaagaagcat gtcaagaggc ttagaaggag ttgggataat atgaaaaaga
     3241 tgtggaaggg gaaccacgcc attgcagaag cagccctaag aggaggttgt gaattttatg
     3301 caggatatcc aattacacca caaacagagg taatggaatt tttatcacat agaatgtctg
     3361 aacttggaag aacttttata cagtccgaaa atgagatggc tgctatatat atggtatatg
     3421 gagcatatgc aagtggaatg aggtctatga cgagttcttc aggaccagga atatctttaa
     3481 aacaagaagg gatttcttac ttatgtgcaa atcattaccc ttgtgtaatc gtaaatgtac
     3541 agagatgggg gcctggtctt ggctcattgg actcagcaca gacagactat ctaagagata
     3601 ctagaggggg aggaaatgga gattatcatt tgatagttta tgcaccaaac agtattcaag
     3661 agactgtaga tttgatgtat aactcatttg atgtagcgga gaagtacaga gtgcctgtcg
     3721 aaattttgac agaagcagct ttaggtcaaa tgatggaacc agtagaattt ccagagttta
     3781 agaagagaga aggagattta ggctggactt atgatggaag taatagagac catgcaaagg
     3841 ttgcagataa ccaaaagcca actttttgta tggagaaacg tatgagaata actgaaaatg
     3901 aacaacaatg ggaagattat caagtagaag atgcagaata tgtgtttgta gcgttcggta
     3961 taccatcaag aacaactatg aatgctgtta gaagacttag agaacaaggt gaaaaagttg
     4021 gaataataag accaataaca gtatggcctt tcccatataa agcatttgaa aaggtaagta
     4081 aagatgtaaa aggatttatc tcagtagaat caacagacac aggtcaatta gtagaagatg
     4141 ttgctttaac ctcaaagaaa gtatgtaaag aaaatgtacc tgtgtatggt ttatttagtg
     4201 gaaatcatat accaaaaaca ttgcaagtaa tggacactta tagcaaaata aaatctggcg
     4261 aaatcaagga ggtgttctaa tatggctaca gaaaaattaa cacctagcgt tgtttcaata
     4321 ccaaacaagt tttgcccagg ttgtggtcat ggaatagtaa atcgtataat agcagaggta
     4381 atagaagaac atggatatga aaaaaatcat gtattaacat taggagttgg ttgtgtctgc
     4441 aatatgaatt ttagctggaa tggtgataag atgcaaactg cacatggtag agcttcatct
     4501 acagctatag gagttaaagt agcacttcca gatacattag taatgactta ccaaggtgat
     4561 ggagatgctt atgttatagg tctttctgaa actttaaata cagcatatag aaatcataat
     4621 gtgactgtat ttgtaataaa caataataat tttgcaatga ctggtggaca aatgtcatgg
     4681 accactatgc cgggtcaagt cacaacaaca agtgtaaatg gaagagatat aaagactact
     4741 ggtagtccac tgaaagttcc tgagatggtg gcaaacttct ttgatgtagc atatgtagca
     4801 agaggttcag ttcatagccc aaaggaaata attaatctaa aaaaatatat aaaaaatgca
     4861 atagaagctc aattaaatgg agagggatat tccttagttg aaatattagc accttgtcca
     4921 actaactggg gtgtatcact agagaaatct atcaaatgga tggaagaaga aatagtgcca
     4981 tattatgctc ttggcgaatt taagcaaagg gatggtgaat agttatggaa aaagagctag
     5041 tatttgcagg gtttggtggt caaggagttt taactcttgg tctgattgta gctgagtcag
     5101 cacttgaatt ggggaaacaa gtaacttgga tgcctgcata tggtccaact atgagaggtg
     5161 gaaaagccta tagtgtagta aagttttctg atgagagtat aggtgggcct gatatggaag
     5221 aaatagatgt tttagtagca atgaataaac catctttaga ttatataaat ttagttaaag
     5281 aaggtggaac agtagttatt aatacatctg caatagatga gaatgtaaca ttaagagaag
     5341 atataagtgt agttaaaata aattgtcagg aattagctca aaaagtaaac aatccaaaag
     5401 cagcaaatat agtagtactt ggagcattaa ttgctaaaac agggttattt gaaaaagaat
     5461 tagctttaaa aactatgtgt gatttctttg aggaaaaagg aaaaggaaag tttaatgttc
     5521 aaaatgaagc agcattcatg gaaggctata ataacgccat ttgataaatt acccttatct
     5581 taagtgattt atctcaagat ttaataatct ttagtgccaa gagaaacttc atcttgagat
     5641 aaaattcact taaaatcaca actataaaca tataggagcg tgaatttatg agtttggaaa
     5701 aacttattag accaaaaagt attgctatag taggagtgac tgataaactt ggttttggaa
     5761 gaagtgcagc acttagtata gtaaaaagta aggaaacaga tagagtatat tatgttaatc
     5821 ccaaaagaga agaattgttt gggagaaaat gttataagac gatacaagaa gttccagaag
     5881 tagtagactg tgtagtagta tgtacaccta gaaatgtagt tccatctgta ttaaaagatt
     5941 ctggagagtt aggaacaaag gcagcagtgg tatatgctag tggatttgca gaggaaggta
     6001 cagaggaagg tacagattta gaaaatcaac ttatagaaat atcaaataca tacgatatga
     6061 aaatattggg tccaaactgc atggggttgc taaattgtat agataaggtt aatctatggg
     6121 caggaggttc taaatgggat ttagatacta aaaaacctgg tataggaatt gtaggtcaaa
     6181 gtggatttat aactgctgaa attgtttcaa gtgattattt taatatatct tatggttttt
     6241 caacaggaaa tggaaatata gttacactag aagatgcagt agatttcctt gtagatgatg
     6301 attatgcatc agtaatagca atttaccttg aaggtttaaa aaatcctcaa aaatttatag
     6361 atgctttaaa aagggcagca caaaaaagaa agccagtaat aatattgaaa tcaggaaggt
     6421 cagaaaaagg tgctatttct gctgcttctc atacaggaaa tttagctggt tcgagtaaag
     6481 cttttgaaag tatttttgaa aaatatggag taatttctgt ggaaaattta gaacagttta
     6541 tgtgtttagc acaagctttt agtgttttag atggtaattt acctgcaaat tctaattttg
     6601 cagcaataaa cttctcagga ggagaaaata ctatttgtgc agatttagca gaagaaaatg
     6661 gtgtagaact tgcagaaata tcttctgaaa ctaaagaaga aatgaaaaaa tatcttccag
     6721 gatttgcaac acctaaaaat ccactagatg ctacaacagc attattccat gaaaaggata
     6781 tgatagtagg tttattacat acctttgaaa aggatgatag tgttggattt actatggtag
     6841 gtgcaaatat aagagatgaa gaaaatgaaa tgcatgaaac cctttgtaaa gctgtaagtg
     6901 aagctagaga acaaggattg aaaaaacctg tgtttgcagt tcctacactt gaaggaaata
     6961 gatatttaga ttataggact agattagaag ataatcaagt gcctataatg tcatcagttg
     7021 gaactacatt tacttgtttt aataaaatgg ctaagtttat agattatgat tattcaaaaa
     7081 gaactttaga atttaaagct gttaagaaaa gagagtcaaa taatgtagta gcattatcag
     7141 aattagatag taagattgag atgaaaaaat atggagtacc agttccagga caaggtaatg
     7201 caaaaagtat tgatgaatta gatgaaatct taaaatatat taagtatcct gttgttttaa
     7261 aaataaattc ttctgaaata ttgcacaaat ctgatgttgg aggagttaaa ataggaattg
     7321 agaatagaga tgaggcagta gatgcatata atgagatttt aactaatgta aaaaaagcaa
     7381 aaccagatgc taatattgat ggtatattag ttcaagaaat ggtagaaagt ggtatagaaa
     7441 ttattattgg tataactaat gatgaccaat ttggaccaat gttattagtc ggtttaggag
     7501 gagtatttgt agaggtattt aaagatacta cattatatcc attacctata aatcatgatg
     7561 aagctattat gatgcttaaa aaactaaaat catttaagct tttaaatggc tatagaggaa
     7621 gtgagccttg tgatattgat gctcttgcag atatgatggt caagttagga aaatatgctt
     7681 atgaaaataa ggatgaagtc aaggaaatag acttaaatcc agtgtttgta tatcctaaag
     7741 gaaaaggcgt atgtgcagta gatgccttaa tagttaaata taaatagttt ataagttttg
     7801 gtattcaacc ataattaaag caccgactga aaatcacatt ttgtaagtta gatagtgtga
     7861 ttttcagtca ccccaaattt aatttaaaaa tattttaaaa tgaaagtata gttttaagtt
     7921 taattgtgaa atagcactgt ataaacaata aaatactacg tataaaacga ataataaaag
     7981 gtgttttaat gataagaatc aatcttgatt ttcattaaaa caccttcttt attatattaa
     8041 aaatttttaa acgttatatt gtcacaggtg ggtttgttgg agctgcatat gtacgattag
     8101 caaattcttg gtcaacaaag aatagaccac cctctccagc aagtttaaca cgttttatat
     8161 tgtcttcaca atttgtttct tcttctgctt gttcagatat aaaccaaagt aagaattgag
     8221 aagttctaaa gtctgattca gagtttgcta cagctgctaa gttgtttatt aaagctgtaa
     8281 cctttttttc atgagctaac gtacgctcaa gaatatccat tgcagaagta aagtctgaat
     8341 ctggcattgg gatagcatca agctgaacct ttcctcctac attatgtaag tatttgtaaa
     8401 aaaacatagc gtggtctgtc tcttctttat attgaacata aaaccaatta gaaaatcctt
     8461 taagtccttc tgcttcaagg taagaagaca tagaaagata aagatacgca gaatataatt
     8521 catggttaat ctgctcatta agtaattttt ccattttagc tgaaatcatt tttatacctc
     8581 caaattttaa atgctcttat aaaatataat aatattaaat ggaaaaatat tcaataaaag
     8641 tgtttaaata ttttacatat ttaataaaaa tttatactac cattaaaaaa attataactt
     8701 acaatggcta tagagccaat taatggtgca tttgctccaa aatttgaatg ccttacactt
     8761 atttttttat atttagaaaa acttgctgaa gcagtcaatt tttttaataa tgtattttct
     8821 ataaatgtac caggtatatt aaagtcatag cctattatta tagaactaca atcaatcaat
     8881 gtaagtgtat taactaatgc atatgatacg tatgtacaaa attcatcaag caaaaacatg
     8941 gcaagactat ctttattatt tgcctcatct attaacttga ctaaagaaaa atctttacag
     9001 ttaattaatg gggagttagg ataaatatgg ctcaatgatt caattctttt aattagatta
     9061 ctgacacttg tataataatc taaacatcca ttattaccac aactgcacaa tggtccacta
     9121 aaattaattg atgtatgtcc tatttctcca ctttgtccta ggttgccagt atgaagttta
     9181 ttttctagaa ctagaccagc tccaatacca ttcattatgt gtaggtatat ataatttgat
     9241 atatttttac ctaaaccata cattttttct gctaatgcac ccgaattagc atcatttatc
     9301 aaaaatattg gcaaatcaga catttctttt ataaaatcta ctatatttag gtttgataca
     9361 tttccaaaat ctgggggatt tagtatggta ccatttatat catctactgg acctattgaa
     9421 gaaataccaa ttccaattat atctctcttg ttgattttca ttattttagt aaacattctt
     9481 tttattatat cttttaaaac atcatttgat agacatttgg ggtaagtttc agaaagttgt
     9541 tttacaatat taccttttaa atctgaaata attaccttac atatatttct tcttattagg
     9601 attccacaaa tacaaggcga tatattagaa atatctaata aaataggttt acgacctgat
     9661 gaatgtgcat ttaaagttga tgactctatt tccctaatca tattttcatt aataagttca
     9721 gtaactagat ttgataaagt cattttagta agtcctgtag acttagctaa atcaactctg
     9781 gacataggtg tatttgtaga aagtaatttc aatattatta acttattttt aatttttaca
     9841 tctttgatgt tagaaccttg tttgttactc atcataaatc tccttaaata aagttaagtg
     9901 ttttatttat tttcaagtat actatcacct tataatagag taacattttt ttacttgaaa
     9961 atatcattaa ttagaatata tcacattata tatcataaaa tatataaaaa aatgttagaa
    10021 ttaaattaaa agtgaaaagt agattgatat ataaatagta aatacttaat taaaaaaaga
    10081 taatgatagc aataattatt gttaccaaga atctactact tcaagtgtta ccttaggtat
    10141 ttaagttgat agtggtttgc acgtaataaa cttgatgtaa tatatattct taataatatc
    10201 gataaaagat ttacttaaaa ataatataga ttatgtaaac atataatcta tttattgtat
    10261 acaataaaat ttcattttgt aaaacagtga attaaatatg taatatagat atcgattttt
    10321 attacaaatt gttactaaat ataggtacat tagaacaaaa atctagtaaa ttgtaactaa
    10381 aatgtaaatt tttcctttaa tctatacaat attatggaaa ctttttgtaa tttatgatat
    10441 aataacaaat atataattat taaattataa taaatgacta tagcgtagat gtaatactat
    10501 gttatagtca tttttgaatt aaacttgata tatcaagggg gaagaatatg agaacaatta
    10561 gaagagtttt agcattaggt ttaacattag ctatatttct aataaatgta cctaatgtag
    10621 atgcattaac atcagataca attaaaggta acaatatata tgagacggct ggattaatag
    10681 cagataaaaa gagttatgat acagcaatta tggtaaatat ggataattct atagcagatg
    10741 gtctttcagc aagtggtctt gcaggtgctg ttgatgctcc aattttgctt gcacaaaaaa
    10801 ataagatacc aaatgaaaca aaacaaagat taaaaaatgt taaaaagata tacataatag
    10861 gtaaagaatt atcaataagt aagtcagtag aaactgaact aaaaaacaca ggggcacagg
    10921 taactagatt gggtggagat gacagaataa agactagtta tagtgttgct aaagaggtaa
    10981 atggtatcaa aaaagttgat gaagtgatat taactaatgc atataaagga gaagcagata
    11041 caataagtgc tgcacctgta tcagtaagag atatagctcc tatagtactt acagatggaa
    11101 agagtgtacc tttttcaaca agcaatgtaa aaacatatgc agtaggtggg agtatatcaa
    11161 tgagcactag tttagttaat aaaactaatg ctaaaagact tggtggttca gatagatatg
    11221 atacaaataa gaaagttata aaagagtttt atccagatgc atcagaattt tatttgagtg
    11281 atggatatga tttagtaaat gcacttacag gttctacaat tgctaaggaa aatccaattg
    11341 tattggtgtc agaaagtagt gataagtcta tattagcagg agcagataag attactagat
    11401 taggttcaat aagtgacagt gtgtataata agtgtgtttc tgctgcacaa aataatggcg
    11461 attcgtctac aaaaggtgag tcacctatga aaaatgagac aagcatatta ggtcagccaa
    11521 cagctagttt agaagcgtgc ttaaaatggg caaaatctaa aaaagctaat gatttattta
    11581 tagagttaat accaatatta tatgatactg ctgttcagga aggtgttaat cctgttttgg
    11641 cagtagctca atctgcaaaa gaaactggtt tttgtaattt tggtggagta ttagatgcat
    11701 catttaagaa tccttgtgga cttaaaactt ctgtaggagg ttctgatact gataagaatg
    11761 cccattcaag atttgatact tgggaagaag gaatattagc tcaaattcaa catttatgct
    11821 tatatgcagg acaagatggt tacccacttt caaatccagt agaccctaga catgaaaaat
    11881 ctttatttgg taaggctaaa acagttgaaa gtttatctaa taattgggct ggaggtcaat
    11941 atggacaaga tttagtaaga atgatgggag aaattgaggc aacaaaataa ttgaagatta
    12001 tcaaatatcc aataaataag gattgattat tatgaaaaag agtaatattt ttagtatcat
    12061 atttcctaca gtcataatgg tgctaatgtt tttaactttt gctacagata tatttaatat
    12121 tccagatatt caatctaaaa gtatttttgt tgtgggaatg gttttgattt ttccaatttc
    12181 ttttttgata caagggataa tttgtgtatt aaataagact aattggattt tatctcttat
    12241 tgtgtcttta gtaacttata tagtattaat gtatatattt ttaaatgatt ctgcatatat
    12301 atatgtttta ctttatacag ctttttatat gattggacat ataacagcta aactctatta
    12361 caaaattaag acttttaaaa attaaattga agtatataaa agtaacatta ggagaagtat
    12421 ccttaatgat tttctaaaat cagaaatagg tgcttctctt ttttgtaaaa ataaaagtat
    12481 gtaaataaaa tttaaccatt ataattttct tttaacaaat gtatacaata aacttgttgt
    12541 ataattaaga tagataaaat aaagggaggg ataatatgaa tttaatagat aatcatattc
    12601 atactaattt ttcaagtgat ggaaaggatt ccatggaaga tacaataaaa aaagctataa
    12661 gtattggtgt tagatatttg acttttacag accatttaga acatgatgaa gatagaggat
    12721 tttgcataaa ttataatgac tatgttccag tatttaataa gtttaaagaa aaatataaaa
    12781 aggacataga gttattacta ggtgttgaag tgggttacag aaaacatcta aaaaatgaaa
    12841 tagaagaaat aataaattct aatccttttg attttgtatt gtgctcaact catacaattg
    12901 acaatgtacc tgttccatca aaggagtatt ttaaaggtct tagcaaagaa gatgcgtatt
    12961 ataaatattt taatagcata cttgaaacta atcgtgaatt tggagattac aatatatatg
    13021 gtcatttgga ttatatatct aggtatggaa tatattcaga taatagagtt atatacaatg
    13081 actttaaaga tgttattgat gaggtcttaa agtcaataat caataatggg agtggtatag
    13141 agctaaatac ttctggttat agatatggat taaatgctat acatccaaat gaagatattc
    13201 taaaaagata taaagaactt ggaggattta tagttactgt aggttctgat tctcataggg
    13261 tagaagatat atgtaaagat tttgatgtgg cctatgatat gcttaagtat cttgacttta
    13321 aatatgtatc tttatttaaa gaaagagaaa cttattttgt aaatatagaa aaagttaaat
    13381 ctaacaatat agcttagagt gtgttttgaa gtaaattaat ggatagttaa aaagttaata
    13441 gttataatat tgtatcctct agaaaaatac tagaggtttt tattttgcta aaataactaa
    13501 atagaattaa atttaaatag tatagtttat tttaaattga atagtttatt gttgatatat
    13561 tttactatta tatattttta ttgtgatata atagtaaaat atattaacct atctttagat
    13621 tatataaatt attataaagg agggaaattt taaaatgtct aataaaaata aagaaaatat
    13681 aactttaagt aatcaaatca aaggaacaat tcaaaatagt ttaacaaaag aagctatatt
    13741 acatatacca atgagcaatt atgcctatgg ttatgataga gaaacactac atataagact
    13801 tagaactaaa aaaaatgaag cgaaaagagt aattttacga ataggagacc aatatgtgtg
    13861 ggataaagga ggtgcaggag gaggaaattt aaatgcatct ggagttggtt ggtctggtgg
    13921 aacaaatata gtaatgagta aagaagtaga aactgaatta tttgactatt ggatagctaa
    13981 gtgtaagcct ttaaataaac gctcacgata tggctttata atagaaggtc aagaggaaaa
    14041 acttttattt acagaaaaaa gaataataga gttgggaaat gaaaatgatg aaaaggaatt
    14101 atgtgaaata ggtaattttt ttggatatcc atatttaaat tatatagata tacctaaagt
    14161 gccaaattgg gtgaaagata ctgtttggta tcagatattt ccagatagat ttgcaaatgg
    14221 tgacccgtct ataaacccag aaggagtaga taaatgggga gctattccaa ccagagataa
    14281 ttttacaggt ggagatttac aaggtgtgat agaacactta gattatttga gtgatttggg
    14341 aataaacgga atttactttt gccctattac aattggaaaa accaatcata gatatgatac
    14401 ggtagattat atggaggtag accctacatt aggagataaa gagactttaa aaaaactaat
    14461 agaagaagcg cataagagaa atataaaaat aatgttggat gctgttttta atcatatagg
    14521 atattattct aaacagtggc aagatgtagt tcaaaataag gaaaattctc gatataaaaa
    14581 ctggttttat ataaaagata tgagtaaaat tgatactcca atagaacaaa tagatgaaaa
    14641 aaatatacct tatgaaacct ttgggtgtga aaaatatatg cctaaactta atacagaaaa
    14701 ttcagaagta ataaaatatt tattggatgt aggcaagtac tggatacagg aatttgatat
    14761 agatgcgtgg agattagatg tttcaaatga agtagaccat gttttttgga gaaagtttag
    14821 agaagaagtc aaaaaagtaa aaccagacat ttatatattg ggagaaattt ggcatggtag
    14881 tttaccatgg ctaatgggtg accaatttga ttcagttatg aattacttga tgtcagaagc
    14941 catgaaaaaa ttcttttgta cagatgagat aaacgccgaa gaatttaagt atatgataaa
    15001 tgatgtaatg gtaagttatc caatacaagt tagtgaagtt atttttaatt tacttggaag
    15061 tcacgatact acaagaatat tgacttatgc taatggaaat attgatagat ttaaacttgc
    15121 atatatgttt atgtttgtac aaacaggatg tccttgtata tactatggtg atgaaatcgg
    15181 aatggaagga gaattaagtt caatatctga aggtcaaaga aaatgtatgg aatggaatga
    15241 agaaaaatgg aataaggata tatttgattt tatgaagaag attattagac ttcgtaaaga
    15301 aaataaagaa cttaggtcta tatcaaatga atgggtgtta gcagataagg aaaatggaac
    15361 gattatattg aaaaaagaat ctatttctat aataataaat aattcatcta aaaatataat
    15421 tttgacgtta ccagattatt tagcaaataa aaaagtaaaa gatttatatg aagaagaaat
    15481 agttgattta aaagaagaac taatgttaga gaaatacaag ttcattattt taaaataata
    15541 caaaaaagta tttattttag atttcatgtt aaattattat acagtttaaa taagtcttta
    15601 taaacgataa aaaaatatgg tctacaagtg agtgaattga aagtagattt taaattaaag
    15661 actgagttag agtagataat tttataaata atatcttaaa tacaagatag aaaataaatt
    15721 gattattagt ctaaaaattt cttgtagaaa gaggaaataa gaatgattga agcatttata
    15781 tttgatttag atggtgtaat tacagatact gcatattatc actatatggc ttggagaaaa
    15841 ctagcccata aagttgggat agatatagac acaaaattta atgaatcttt aaaaggcata
    15901 agtagaatgg agtcattgga caggatatta gaatttggaa ataagaaata ttcattttca
    15961 gaagaagaaa aagtgagaat ggctgaagaa aaaaataatt actatgtatc attgatagat
    16021 gagataactt caaatgatat tttgccaggt atagaaagct tgttaattga tgtaaaatct
    16081 aataatataa agataggtct atcttctgca agtaagaatg caataaatgt attaaatcat
    16141 ttgggtataa gtgacaaatt tgattttata gctgatgcag gaaaatgtaa aaacaataaa
    16201 ccacatccag agatatttct tatgtcagcg aaagggctta atgtaaatcc acaaaattgt
    16261 atagggatag aggatgcaag tgcaggtata gacgctataa actcagctaa tatgttttct
    16321 gttggtgttg gaaattatga aaacttaaaa aaagcaaatt tagttgtaga ttcaacaaat
    16381 caattaaagt ttgaatatat acaagaaaag tataatgagt atatagttag gagaattata
    16441 tgaaatgttt atttagtaat gtagatgagt ggaagattat acaagataag attgaaatca
    16501 aagaaaatag attagcagag tccataatga gtataggcaa tggatatatg ggtatgagag
    16561 gaaactatga ggaaaattat agtggagata cacacaaggg gtcttatata gctggagtat
    16621 ggtttccaga taaaactcgt gttggttggt ggaaaaatgg gtatccagaa tactttggta
    16681 aaattcctaa ttcagtcaac tatataggtg taagaatttt catagatgat gtagaattag
    16741 atttagcaaa atgtaaagta gaagattttt atagagaatt agatatgaaa aatggtattt
    16801 taaatcgtag gtttattgta aatataaatg ataaaaaatt tgaagttagg gttgctaggt
    16861 ttttaagtat atctgttaag gaacttgcag taattaaata cagtataaaa tcattaaatt
    16921 ctaatactaa aataaaattt gtaccatatt tagattcaaa tataaaaaat gaagattcaa
    16981 actatgagga aaagttttgg aatggaattt cttcaagttc tttagaatat aaaggtagta
    17041 ttctttctcg aacaaaggag aatgcattta atacaccagt attcactgtg ggttcaatga
    17101 tgtatattaa gacaaatggt gatgtagaag ctgtatctca tgagagtgat tataattact
    17161 gtgaaaattc gataacaatg gatgttttaa aaaatgaggt tgcaagtata gaaaaatatg
    17221 tatctataac ttcaactaga gattataagg aaagtgagct attaagaaaa tgtgaagaca
    17281 ttttaaatag agaactatct aaaagttatg atgatatatt aaatgaacat acaaagttat
    17341 ggaataaaag atgggaaagt gcagatgtaa aaataaatgg agataaaagt agccaacaag
    17401 gtataagata taatttattc caactatttt caacatatta tggagaagat tcgcgattaa
    17461 atataggtcc aaaaggattt actggtgaaa agtatggagg agctacttat tgggatacag
    17521 aagcgttttg tgttccagta tatctaggtg tagcagataa ggaggtaact agaaatcttt
    17581 tagaatatag gtacaatcag ttagaacagg caaaggataa tgcaaaaaaa ctagggctta
    17641 aaggtgcttt atatccaatg gtaacatttg atggagtaga atgtcataat gaatgggaga
    17701 taacatttga ggaaatccac cgaaatggaa gtatagtata tgcaatatat aactatacaa
    17761 attatacagg tgactataaa tatataaaag aaaaaggaat agatgtaata atagaaatag
    17821 ctagattttg ggcaagtaga gttcatttgt ctacaaaaaa agatttgtat atgatacatg
    17881 gagtaacagg tccaaacgag tatgaaaaca atatcaataa taattggtat actaattata
    17941 tagctaaatg gtgtttagat tatgctgtag aaaatatctt acgattagag aaagagtgta
    18001 atgaatgtat tgacagaaat aatgtgacta gggaagaagt tgagttttgg aaaataatat
    18061 caaataaaat gtatctgccg tatgatgagg aactacaaat aataattcaa aatgataatt
    18121 ttttagataa agaatttgta gaagtaaagg atttaccaaa agaaaatttt cctcttaatc
    18181 aaaattggtc ttgggataag attcttaggt cgtgttttat aaaacaagca gatgtattac
    18241 agggaattta ctattttgga aatagattta ctaaagaaga aaaaaagaga aattttgact
    18301 tctatgaaaa atatactgta catgaatctt ctttatcttc tagtatttat tctattattg
    18361 cctcagaaat agataaccta gaaaaggctt atgaattata ttcaagaaca gctaggcttg
    18421 atttagataa ttacaataat gatacaaatg atggactgca cataacttca atgtctggtt
    18481 catggctttc cattgtacat ggatttgcag gcatgagaac atggaatgaa actctatctt
    18541 ttgaccctaa gttgccaaaa gagtggaaaa attattcttt caatataaat tatagagata
    18601 atattattaa tgtagatgta tgtagtgatt taattaaaat agaaaattta aaaggtagac
    18661 caataaaaat tattgtatat gggaaaaaat atatattaga aaaatgtata aaaattgata
    18721 aatagattta ataatatttt tattatataa atagttaaaa taagcta
//
LOCUS       DAEMGG010000074        18369 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEMGG010000074 DAEMGG010000000
VERSION     DAEMGG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.074"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(220..900)
                     /locus_tag="KPK24_002380"
     CDS             complement(220..900)
                     /locus_tag="KPK24_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300048.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(950..2965)
                     /locus_tag="KPK24_002381"
     CDS             complement(950..2965)
                     /locus_tag="KPK24_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2300049.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(3037..3714)
                     /locus_tag="KPK24_002382"
     CDS             complement(3037..3714)
                     /locus_tag="KPK24_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300050.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(4294..4377)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(4610..5452)
                     /locus_tag="KPK24_002383"
     CDS             complement(4610..5452)
                     /locus_tag="KPK24_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2300051.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(5704..7488)
                     /gene="pepF"
                     /locus_tag="KPK24_002384"
     CDS             complement(5704..7488)
                     /gene="pepF"
                     /locus_tag="KPK24_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG2300052.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLVHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(7554..8219)
                     /locus_tag="KPK24_002385"
     CDS             complement(7554..8219)
                     /locus_tag="KPK24_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG2300053.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(8370..8531)
                     /locus_tag="KPK24_002386"
     CDS             complement(8370..8531)
                     /locus_tag="KPK24_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG2300054.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(8609..9118)
                     /locus_tag="KPK24_002387"
     CDS             complement(8609..9118)
                     /locus_tag="KPK24_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG2300055.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            9324..9542
                     /locus_tag="KPK24_002388"
     CDS             9324..9542
                     /locus_tag="KPK24_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2300056.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            9560..11506
                     /gene="feoB"
                     /locus_tag="KPK24_002389"
     CDS             9560..11506
                     /gene="feoB"
                     /locus_tag="KPK24_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG2300057.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(11685..12740)
                     /locus_tag="KPK24_002390"
     CDS             complement(11685..12740)
                     /locus_tag="KPK24_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2300058.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(12788..13588)
                     /locus_tag="KPK24_002391"
     CDS             complement(12788..13588)
                     /locus_tag="KPK24_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2300059.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(13581..14372)
                     /locus_tag="KPK24_002392"
     CDS             complement(13581..14372)
                     /locus_tag="KPK24_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2300060.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(14412..14834)
                     /locus_tag="KPK24_002393"
     CDS             complement(14412..14834)
                     /locus_tag="KPK24_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG2300061.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(14850..15329)
                     /locus_tag="KPK24_002394"
     CDS             complement(14850..15329)
                     /locus_tag="KPK24_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2300062.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(15461..18217)
                     /locus_tag="KPK24_002395"
     CDS             complement(15461..18217)
                     /locus_tag="KPK24_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2300063.1"
                     /translation="MRDEILSFVNECVLFNSFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGIAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         6011 a         3149 c         2021 g         7188 t
ORIGIN      
        1 ttagatatac aatttatcca tcttttcaaa tataatctat aaaataatag ttccattacg
       61 cattttttta tataataaaa ctgactgtaa tttaactggc aaagtctaga agaataggat
      121 ttatcaatta aattacagtc agctatttta taataattta gtttagatat cgttagtatt
      181 tcacatttat aacaattatt aatgatatta atatatttat tattccttat taaaataata
      241 tcctgctcct cttattgtta taattttctg aggattttta taatctttct ctattttctt
      301 tcttaatcca ttcactgcaa ctaccacact atagttatcc tctaagtatt cattcatcca
      361 tacatgttca tatatctctt tagtagtcaa agtcttttca ggattctcta taaaaaagtt
      421 taacaactta ttttctgttg gagtcaattt tattatttta tcttctaact ttattactcc
      481 actagttcta ttatattcct ctaattttgt attattttca ttattattta catatgagtt
      541 cattcttctt aaaatagctt ttacttttgc gtataatatt tctaaattta gaggctttat
      601 taagtaatcg tctccaccag aatttaaagc atttaaaatg ctttggtctt catttatata
      661 agtcatataa acaattggac aagtagttac atttctaatt tttttacaaa gagtccatcc
      721 atcaccatca cttagaatta tttctaaaaa tattaaatca tatttattgc taaatatttt
      781 tccaatagct tcttcacaat tatatgctaa atctatactt attccttttt cctccagaaa
      841 tccttttagc ttctggcaat tatccttgtc tccatctatt atcaatatct tagtttgcat
      901 tataatcact cccaaatatc attattcttt tatattaaat aagctattat tataaaaaac
      961 atagacttaa ttttattgta aaaacagttc cttttcctag ttctgaatct acaaatattt
     1021 ctccaccatg ctctcttata atggatttga caatatatag ccctaaccct tgcccttttg
     1081 gctcattatt gtctctttct gtaaagaatc tgtcaaatac ctttggtata ttttctggag
     1141 gtataccttt tccattatca gaaattttaa taattgcaaa tccatcttct ttatccattg
     1201 ataaaattat ctttccatca aatggagtaa aggtcaaagc attataaatt aaattctcaa
     1261 atgctcttgt aagcatctct ttatttatct ttgcatatat ttttttctcc aatacataag
     1321 tttcaaagtt tacaccacat gcttgtgtat ctggcaaatt attttcatat atctctttaa
     1381 taaaattatt cagacatatt ttttcactaa taccaatgct tttatcttgt gatgtaaatt
     1441 cttgcaaact acgtactcta ttttgtactt catttgactt ttgatttata gcataaagat
     1501 aggttcttgt ctcttcatct acatgaatat taccaacctt tatcaaatct ataaatgctt
     1561 gtatagccga aacaggtgcc tttaaatcat gtgctacatc tgccaagaat cttttacgtt
     1621 catttaacaa tgttgtaagt tcattagttc tcacttcaac ttcatgttca agattccttg
     1681 taagtttttc attctctttt attattattc tattcctgtt tatcatcata acagaaaata
     1741 aaataacaat tacaaaccca caatattctg tttgccaccc tgtaaatata ggctcaaatc
     1801 tattactcgt aactgcatca aataatatgc taattccaaa aacagtgttt actgaaacta
     1861 aaatataaga acttatatgc tcagagcatg tctttttata tattgaaacc agcgaagaca
     1921 ttaatatata tataaataca atatatttat atatgtctat taacttccca tatatgttga
     1981 caaagtacat atcatatgga agaagaataa ttggtatcac tatagatatg atactcattg
     2041 taattcctaa tggtaaaatt aaaaatctat acactttatt ttcttctatt ttggatatac
     2101 ttccatttat acaaataata cataaaacta ccataaaata tgacaaatct tcaaccgcat
     2161 atgtaaagct taccaaaggc agtcctaaca aatgtacaaa gggataggat acatggatgg
     2221 caaagaaaac acacatacat ccaaaatata agtaaatttt acttttgttt gaaagaaacc
     2281 atacagacaa tgaaaatata gctattgcaa aagttgtgaa gcaaattata ctataaaaaa
     2341 gtaatcgata aaacatcata ttacttatgt cctttgtttc tcctaatgca ggcggataat
     2401 acaaaccact ataataatgt gaaaaattcg atacattgat tataatttct gtatttttct
     2461 ttgcaataaa acttactact gaatttttaa ttttgggtct atatttttct gttcctacat
     2521 ctcctaattt agaaaccatt tctccattta tccatacttc acttgcggaa aaaatctcag
     2581 gaagctctaa acttagtagc ttatccttgc cttcattttt tattctcata cgataagtag
     2641 cattcccata tggtgactga gtcccttttc tcatagaaaa atctggatat tgacctataa
     2701 aaacatattc tggttttata tcttgctctg taaaatcatt cggtttaaat attttatgat
     2761 tataaaactc ccagtcatct atcaaaaaga caagtttacc tttagtgaaa gtatcattat
     2821 ctatagttaa aattccatta cttgctctaa tagacttata tgtatattta ttatcatatt
     2881 tatacagaag atttaatcct gttattgcca gcagagagac tatcaataca atgagagttc
     2941 cttttagtat attatacttt ttcattgtat caaccacatc tctttcatag tagtattaaa
     3001 taaaacaaga actatttatt aattacagta tctcacttat ggtacaaact tatatccttt
     3061 tccccatacg gtttgaatgt attgatgctc cctgcttaaa gaattaattt tttttctcat
     3121 ctgtgcaata tgaacctgca ctgtatgttg atctccaata tcaggcattt tccaaacttg
     3181 gttatatatc tctatctttg aaaatacttg ttctttgttc tcagctagaa acaataatat
     3241 ttcaaattcc atagtagtta aatctatact ttctccatca aaagttactt ttcttgaatt
     3301 tacatttatt actaacgctg aaaactttaa cacattagga actgatgtaa tatttttgta
     3361 ctgcctcata cgagcatata ttctaagttc aagttctttt aaactataag gttttgttat
     3421 ataatcatcc cccccagcta aaaatccccg aactttttct tcttcatgct cataattaga
     3481 taaaaatatt ataggtatgt taaccttttc ttttatatca ttgcacattt tatatccatc
     3541 tatatctggc aatgaaatat ccaaaattat acagtctaaa actatactct ctactatatc
     3601 catagcatct ttagcagtct ctgatgtata tacattaaaa cctatattag aaaaataaat
     3661 ttgatttatg tttaaaatat caacatcatc atctacaacc agtaaattat acattacacc
     3721 actccattca aaggtatttt aattataatt ccaagtttta tagttattac aaattataat
     3781 gttttatttt catatataac tggtattatt attttataat atctttacaa actagactat
     3841 aatacttaag tatttttgaa taattcttga atattgtatt aaacattaag attcattatt
     3901 aaatactaat taaagtctcc gattgttgga gaatggagct taaagtcatt ttcacgtata
     3961 aatttattat ttttctagaa acttgtctat tggctagttt gagtattgat ttatatgatt
     4021 tatatggtta taatataaaa taaattgtat tattttggta aatatattgt tacaaaaggt
     4081 ttatattctg catctcaatg tttataaaaa ctttattttc ttattataat actattattc
     4141 ttttaaaaaa atttcccttt tccaacaaat ggctacttta aaagccaaaa ttttacctat
     4201 caataaaaat cttaacgact aatactctta ttttcaaatt ttgcaacatt tttgttaatt
     4261 ttaaaacaaa aaaagactat gcaatataaa aattatattc atagccggtt gcaccactaa
     4321 ctccatatat ctctagatgc ccaataataa gatatacttc tcagtttcta aaatattttt
     4381 attaaaaatc ttttatatat tttcataaat ttctttttta ttctaacatt gtaatattat
     4441 tttgtcaata tttccatatt cttagagata agatactaaa atacctattt tatctaaaga
     4501 tatttatatt tctacattca ataatagaca ataccctgca agttaaagca tcatttttat
     4561 gagcttatct cacagggtat tatatgttta ctttatatta taactcttgt tattgattaa
     4621 ctttaataaa tcctttttct gaaacaagtg cttgtccttc atcacttaat atataatcta
     4681 ttaaagcttt acctttttct gacaaaccat cttctttatt aactaatata aatggtcttg
     4741 atattggata tttattagct attacatttg catcagttgc ttcaacatca tttattttta
     4801 aactatttac tttctcatca acatatccaa atgatatata tcctatagca ttttcatttc
     4861 cttcaactgt agtttttata tttcctgaac catcacttat ttgtgcatct ttagttagtt
     4921 cttctgaatc aaaccctaca atttcttgga atccatctct agttcctgaa ccttcttctc
     4981 ttgatactat tactattggt gcatcatttc ctcctacttc tttccagtta gtaatctttc
     5041 ctgtatatat acctttaact tggtccattg tcagattttt tactgagttt gcagtattag
     5101 ttatcaatgc tataccatct attgctattt tagtttcctt taatccagca cttttttctt
     5161 catcttttaa atctcttgaa ctcattccta tttcagatgt tccttctata gcatttttta
     5221 tacctccaga agaaccaatc tgttgtactt ctacactaac accttgattt ttttcttgaa
     5281 acttttctcc gatagtttcc ataagaggac ctattgaagt tgaacctgaa acagaaactt
     5341 tgcttgaatc tcctgaatca cctgaacctc ctgagttaga actacatcct actgctaaag
     5401 ctccaactaa cattgtacta gctaaaagaa cgcctatttt ctttttaaac attttactta
     5461 cccccgctta tattttaatt ttttgttgtc atttttctat attttaataa tacagattct
     5521 ttgttaaatg aatattagag cagggttaag taaatgtaaa tattgttaaa tacatgtaaa
     5581 aacttaattt tatatgattt attcttaatt agattaactt tttaaagtta ctttaggcag
     5641 atataaacat aatacaactc atattatcag aagtcttcat tgaagctatc aacctatcac
     5701 atcctataat tctttttcta gtttattaac taattcagca aatacattta aggcttcctc
     5761 tatagattct tttttcgtca tatctacacc agcagatttt aattgttcta gtgggtattc
     5821 tgaccctcca ctttttaaga agtttatata tctatctact gcagtacttc cttctgaaag
     5881 tatctgttga cttaaagcac ttgcagcaga aaaccctgtg gcatacttat atacatagaa
     5941 gtttgaataa aaatgaggta ttctcgccca ttctattcct atttgtttat ctactattgc
     6001 agattttccg taatactttt catttaattt gtagtatata ttagtaaatt catcagcagt
     6061 aagtggttct ccactctcta ccctttgatg tacaattttt tcaaattcag caaacatagt
     6121 ttgtctatga actgtagttc taaattgttc taaataataa tttaataagt atatagtttc
     6181 atccttagac ttagaatttt ctaataaata atttataaga agtaattcat ttaatgtaga
     6241 tgcaacttct gcaacgaata tcttatagtc cgaatataaa aatggttgac ttgttcttga
     6301 ataatgactg tgaacagagt gccctaattc atgaactaaa gtgaatagag aattaaggtc
     6361 atttttatag ctcattaata tataaggatg tgaatcataa cttccccaag aataagctcc
     6421 tcctttttta ccttcattat catatacatc aatccaccct tcatcaaaag cactttttat
     6481 gaccttcaag tattcttctc ctaaaggttt aagtgcctct aatatgatat cctgtgcttt
     6541 atcatatggt atatccatgt caaatttatc tgtcaaagga acatacaaat catacatgtg
     6601 tatttcattc aatcctaaaa atttcttttt aagctcaaca tatttattta gattaggaat
     6661 acttttatct atagctgata taagattatt gtaaacatct aaacttacat catctgaaaa
     6721 taaagacgct tctattgctg aattatactt tctagttttt gcgtaaaata tctctgattt
     6781 tattccacca taaagcgttg aggcaaatgt atttttatat ttctcatatg ttgaaaattc
     6841 accttcaaaa gcgtcttttc taactctact attcttactt tttataaata aagagtagtt
     6901 tgaatgagaa agcttaacct tattcccctc ctcatcttct atctcaggaa attccatatc
     6961 tgcatatgct aacatatcgt atacattttc tggaattgtc gataattctg atgtagctgc
     7021 aagtatttct tcctctcgtt cactcaaagt atgtggttta tctctcaata tatcctttat
     7081 atattttgta taaaaggata gcttctcatc ttttaagtat tcacttaact tattctcttc
     7141 tatcgccagt atttctggaa ctatataaga agtagccaca cctaaatcag tagataacat
     7201 ttctgtctta gtggctcttc cttgattatc attaattcta gtatcttcat gttgtttcat
     7261 atgagtatat acatataaat tttgaacaat cctagatgca ttttcagaga tatttaatac
     7321 tttatataag ttctccttac tctcagaaag cttcccttta tattctttta cttcttggat
     7381 aagatttttt actttactta tgtctttttc aatttcctct ttactggagt acatcttatc
     7441 tatcttccat ttgtactttt cttctattgt acttctatca gttcccatat catacacctc
     7501 tattttaatt tgttttatat tttattcact ttacaaaata tattgtatat attttagtcc
     7561 aaataatcta catttattac tccttctatc atatttattt ctttcataac atcgtgtaag
     7621 tgtattctct tagcataaga tacaaaaaaa ttaaccttcc agtattcttc attacgacat
     7681 tctatttcca tatttcttac gataagttga tttttttcaa atttatcata taaaaaaggt
     7741 atagagtcca aattgcttaa ttttaatatc aatacattct tttcttggct aaataaaaac
     7801 gtattgatta tcctaagcac aaataatgct atcattacaa ataatccacc tagaattgcc
     7861 aattcataat aaccatatcc aactgctata ccaatacatg cagttgccca catcccagca
     7921 gctgtagtaa gcccttttac cccaccttta ttttttaaaa ttgctcctgc acctaaaaaa
     7981 ccaattccag ataatacttg agcaggcatt cttgctgggt cagcatttgt atacaaatta
     8041 taatcaaaga ataattgaat cgaagttatt gaaacaatac aagaacctat tgcaattagt
     8101 atatgtgtcc taaatcctgc aaactgtctt attttttctc tctctaagcc tattattcct
     8161 cctattatta gtgctaatag taaccttatt gctatctcct tacttcctat atcaatcata
     8221 atataaaacc ctcctgactt aattacaact tatgtcctac agataattta cacatcagaa
     8281 atactatttt atatcttaat attattatta atatatgtaa aatatagcca tctgttaatt
     8341 tttgatacaa aaaaacccac aactgtgggc tatttgctta ggtacatgta tttatttatt
     8401 aatatatcta ttttcttact tgattctact aactcttctt gaaatatttc tggatattct
     8461 atgtatgtat ttatctcttc cctaactgct tcaatctcat tttttaacgc ttctaattca
     8521 ttgttcgcca taataatcac cactattaca aatttgtata tttttatata cattttatca
     8581 aacaatatta tattttgtca atattatgtc aataaaactt actataaaaa tgctttaatt
     8641 taggtattgt atacctggat actaagccta caaacatacc agttactaat gatgccatta
     8701 acataaatgg taagtaccct atagcaagta tattttctat gattaaactt gcaactatta
     8761 attgacctat attatgccct attgcaccta taatgcttat ccctattagt gataaatctt
     8821 ttattttact cgctaaatac ataaataaaa gactcagata agctcctcct atactaaata
     8881 gtagatatga taaagggcct gcaaatattg atgacaaaat taccctgata gtaacaatta
     8941 taacagtatc tttgacacct aacaacatta atgaaattaa agttataata ttgctcaaac
     9001 ccaattttgc ccctggaaat ataactataa gaggatttgg tatatatgtc tctaatacat
     9061 ataaagctaa actataccct atcataagac ctaaaaatgt catttttcta gttttcatta
     9121 agttaactcc tccaaatttt atctatcatc ttacattata atacaaaata tataaaaaca
     9181 cctaatttag actaaaatat ttttaatcta tttagaataa atattagtta tttcaatgtg
     9241 caattacctc tataaatctt gactttttgt atcatacttg ttacaatttt aatgataatg
     9301 aatttcatta ggaggtgtta ataatgactg tttacgattt aaaaatagga gaaaaaggat
     9361 ttattaatag tatagatgga gatattaaat tatctaaaag attgcttgca cttggatgta
     9421 taaatagtac tgaaatagaa gttaaaaagg ttgccccact tggagaccca attataatta
     9481 gatttagagg ctttgacctt gcgattagaa aatctgatgc taaaaatatt tctttacaat
     9541 aatagaggag gagctacccg tgattaatgt agcattatta ggaaacccaa atgtcggtaa
     9601 aactactgtt tttaatctac ttacaggttc aaatcaatat gtaggtaact ggcctggtgt
     9661 tactatagaa aaaaaagaag gatttcttgg aaaagagata aaggttgtcg atttacctgg
     9721 tatatatgca atggatactt tctcaaatga agaaaaggtt tctaaatcat atttagaaaa
     9781 tgaagatgtt gatgtaatag taaatgtagt tgatgcttct aatttatcaa gaaaccttta
     9841 cttaacaact caacttatgc aatttaacaa gcctatagtt atattgctta atatgttaga
     9901 cattgcagaa tctaaaggag ttaatataga tgccaaaaaa ttaagtgaag aacttggagt
     9961 aattgttgtt ccaattatag caaaaaagaa aaatggtgtt gataggatag aagaagagat
    10021 taagggagct acgcaaaaag cttttatata tcagaaaaac tttggaagtg aaacagaaac
    10081 atataaaaaa ttagaatcca ttttatcgag atgtacaaaa actacatata cacaaaagaa
    10141 atctatcagt gataagatag acaatatagt cttgaatcct atattagcat atccgatatt
    10201 tattggtgta ttgttcctct tatttaaatt tacctttgac tgggtcggag gaccactaca
    10261 agagggattt gctagtctta tagaggctta tatttctgca cctgttagtg atatgttatc
    10321 taactctagc ccatggttta aatcacttat agttgatgga ataattggag gtgttggtgg
    10381 tactttacca ttcttccctt taatatttac attattcttt ggtatatcgt tgtttgaaga
    10441 tagtggatac atgtcaagaa cagcattttt aatggataaa gttatgagaa aggttggtct
    10501 atctggtaaa gcttttatac ctatagttat gggtatggga tgttcttctc cagctataat
    10561 ggcaactaga acattggaaa gcgaaaaaga tagaagagtt acagctctta ttgcaccact
    10621 tatgacttgt ggagcaaagc tacctatata tgcactattt gtagctatat tctttccaca
    10681 taatgctgct ttagtaacta catcactgta tttggtgggt atagttatgg caattgtagt
    10741 tgcattattc ttaaataaaa cagcctttaa aacagaggct gaaccattta tattagaatt
    10801 accagaatat agaatcccta ctatagatgc acttattaaa aatacttgga ataaatcaaa
    10861 aggtttcttg ataagagttg ttactgtcat gtttgcaatg tcagtcgtaa tttggggact
    10921 ttcttcattc aatgtttttg gatatacaga agatataaat gtaagcttct tggctactct
    10981 aggtcatatt atatctccta tattcaaacc tcttggtttt gatgattgga gaacttcagt
    11041 tgctatttta agtggtcttg gagcaaaaga aatcgtagta aactctttaa atatactata
    11101 tggtaatcta actgttgtct taccaacaat atttaatgga gttactgcat atacattctt
    11161 gatatttact gctttatata ctccatgtat agctgcactt gcaactatga aaaaagaata
    11221 tggcaataaa atgatgttta catcatttgc atatcaattt atactagcat ggataatggc
    11281 ttttatagtt aaaagtatag gtggagtact atttatgggt aattcaataa tggaagcttt
    11341 gataggtgga ataattatag tccttgcact ctttattctg tttaataaat tcaagtctac
    11401 aaaaaacgga aatgtctgta gtggatgttc tatttgttct ggatgtccaa gtgctaattc
    11461 ttgctcaagt aaggaagaca gcaataaaaa aaataatgca cactaatata taaatatttt
    11521 agtttaaaat aagataaaag tgggtgtctc aaaattaact ttttagttca tgaggcactc
    11581 ttttttatat aaaatcaaat atattttcat catttcaaca gtaaaaaaga gcttatctag
    11641 taaaaaagag gcttatctct attttgagac aacctctttt tttgttactt attcagtttc
    11701 ttgctaccta acttcatgtg gaacctagta tcttttggaa cacttgggtc tacacctctt
    11761 aatgtaggta tttccccaca tattagttgt ataggtacta taaaattaaa tgtttgatag
    11821 taaggattat ctgtgatatc aatctttaaa tttttatcat tttctgtaac atctctacct
    11881 atagcaaata cattttccgt ccaaccagaa agtacatcaa tcatcttagt tactctaggt
    11941 tcttttcctg tatctagtat aaatatagta gaatcactat ttatagcatt atatatacca
    12001 tgtataaact cttcaaactc atacccagtt acaggtattc tcattgtttc caataatttt
    12061 aatgctgctt ctaatgtatc tccatatata tcagagtgac ctattattct tatttcttta
    12121 gaattaacta acttttcttt gtttctttca atccattgtt tagagagctt atacactgct
    12181 tcaaatctat taattgcgtc taatatctta tttatttcct cattgtactt ttcactagat
    12241 attatacctt tttctctagc tatttgaaga cctaataaca ttaagtttaa ctttgtacaa
    12301 taatatcctt ttgttttagc tccagatttt tcttcaccac aatttactgt taatatataa
    12361 tcagaaatct catctattaa agcattctta caacctgcca tagatgctat tttacagccc
    12421 ttatcttcag ctaacttcat agcgttgtat gtagaataac tactcccacc ttgagataca
    12481 cctactacta gggtattttc attatcaaac ttgaatgtat cttcagttat cataaatgga
    12541 tacattttca caacatctat atctaataaa ttttgtaaat atggttgcac ctgcactcct
    12601 gagtgatagc tagtacctga acctgttatt ataatctttt ttagatttgt tctcttaact
    12661 tcattaaata agctatctgc attagatata atttctctca ttctaactgg tgtttctaac
    12721 atataatctt gtattgtcat tttatacacc ttccttatct atatattcaa taaattataa
    12781 ttaatttcta taacaaacct atagcatgta aagcgattcc tgttactata gtgcctatag
    12841 ttaattttgt tacacttacc tttctcttta acatgtagta tattacaaat gcaaaggtta
    12901 aaggtaacat attaggtatt attttatcta gtatatcttg taaaattact tctgcaccat
    12961 ttaagttgaa tttcagagga gtagttatac tcaacatagt tgcaaccatt cctcctacaa
    13021 cgaataaacc aacagttgta gccatagaca ttattttctc cataagtccc tctctttgta
    13081 ttctttcaag agaatttacc cctacttgat aaccatactt aagtccaaat attctaaggg
    13141 caaatgctgg tatattgtat aatagtaaga ataatagtgg tccaaagata ttaccttctt
    13201 ttgcaagtga tactcctact cctgctgcta taatcctaaa tgttccccag aataatgaat
    13261 caccaattcc tgcaagaggt cccataagag ctgttttaac agcactaatt gaactttcat
    13321 caaattctgg attattagca ttctcttctt ccatagcggc tgttatacct aatatagcag
    13381 gaactaccac tgtcgttgta ttaaaaatct ccaaatgcct ttctattgct tttgcttgat
    13441 tttctttctt attgtatagc ttctttattg caggtaacat tgaataacag taacctaagt
    13501 tttgcattct ttcgtaatta aatgcacctt gaagagcaaa tgaacgccaa aacatacttc
    13561 ttaattcttt ttttgttaat ttattcaaag tcaatctctc cttccgttaa agtattgatt
    13621 gctaattgag gattttgatt attttctttg ttattgatat aaaagtttag tacaaatgct
    13681 acacatactc caaataaagc tatagcagtt atatctaatc caccataaga tgctaacaaa
    13741 aaccctataa agaaaaatac agacatcttt tttgaaagta acatatccat aagcaatgca
    13801 aaacctactg ctggcaacaa tgttcctgca acacccaatc cgtctaatat aactttaggt
    13861 attgcattta ttaatgctgt aactttatca gcaccaatca taattgctaa aaaggttgga
    13921 actgatgtac ttaaaaagaa taatattact ggcaaccaca tcattatggt aactttctta
    13981 aaatcagcct gtttagcata gaaaactgcc ttttgagaaa agtacgaatt tattattcta
    14041 actaatactc caagagattg tgctaagact gctactggta ctgctactgc taaagcaact
    14101 tctgcaccat tacctgataa tatagcaaaa gctgtaccta atacacctcc tgttactaca
    14161 tctggaggag tagctgcacc tattcctgca attcccatcc aaattaattc aagctgtgca
    14221 ccaatcataa ttcctgattt tatgtctcct aaaactaatc ctactaacat acctgtaact
    14281 attggcctat caagcataga ttgaccaaat atacgcccat ctagtatacc aactcctgct
    14341 ataatagcta acatagtagc ttgtactaac atattgaacc tccattttaa cttagtttaa
    14401 tttacattaa attacattac atttttgatt ttgttttgat tttctaataa ctcatttaca
    14461 taaacaattc cattttttgc ttcctctaat cctgatttaa ctattagttt tgtatcctcc
    14521 tcaactaata aaagtataga taaaagcatt ggtaaattta ctcctgtcaa taattcaaat
    14581 ttgaaccctt gtaaaagtaa gtttgtacat atgtttgcca cacttccacc aaacacatct
    14641 gttaagacaa caaattcttc actatcttta ttatttgata atatacttct aatttctttc
    14701 tcagcatcat ctgctgattt atccgttgtc atacaaaaat actcaatatt agttaatgaa
    14761 ccaattatta atttacttgt gtcaacaagc tctctggaca attctccatg tgttgcaatt
    14821 agaaattttt tcattctctt tctcctaaat tattttatta aatctgatag ttgtttcttt
    14881 ttgtctctag gtaacattct aaattctata tcaatcccta aatcacctat ttgtttaaag
    14941 ttatttatat catcatcatc aacagcaact aaatctgtaa gtttcttctt cccttcctca
    15001 tatcttaatc ctcctacatt tatttctttt aatactcctc ctgaattttt agctacaact
    15061 aaagcatctt tggtattttt tactaacact aatacattgc ttttctttga agaagcaaat
    15121 ttttctacta tttctccaga ttcctctacc ccccttatat ataaagttac attgctaggt
    15181 ttcgctaagt tcagtgacat agctttcatt tggtcatttt ttgcttcatc atttgcaact
    15241 agtattacat ttatacctag tgatgttgcc catccatatg caacctgacc atgtatcaat
    15301 ctgtcatcaa ttctcatcaa agataccatt ttccccaaat cctccatcat acattaaaat
    15361 ttttattgtt tatactctca tttatctata aaagcaaaaa gtatgccaaa tactggcata
    15421 cttttttaaa tttcattcac ttattttgta atcttgtatt ttactgtggt agaccataga
    15481 taatattata aatatatcct atctctgaaa ttggtatttg cacactataa ctcttttcta
    15541 gcacactaaa tgcatttttt attagtttta cttcactatg atggtttttg attaacaaat
    15601 ccatatcagt atactcttca atttcagttt gtctaactaa tctttcaatc atacaactta
    15661 agtgaacata taaagaaatc tttttatcat ttgaaagttt tttcttttga atattttcta
    15721 tagcattaat tccttcttct attctctcta ttatcttttt tgaatctaaa atagttagac
    15781 tatctattac tctaattaat gaaaaattct ttacaataga atcatttatg ttctttattt
    15841 tttcattttc tactactctg ctaaatattc taaaaacatc atcttctcct tttcctgata
    15901 ttatatcctc taataatata aaatctaccc cattcacatt tggattggca gttcctacta
    15961 tagccatcac atcatacatt tcaaaaactg gttctttatc tcccatagaa acaagactat
    16021 cataatcata aggtattatt ttgacatcaa ctattccaat taaactttcg gaaagtagat
    16081 gttggatttt tatagcaata cctatccctg taaaacaaga tgatattata gcatattctt
    16141 tatttttttg aggataatac aattgtatat taggtttagt ttcttcatcc ataatttctg
    16201 caatctcttc aatgttttta ccttgcatta ataaatttcc tacttctagt gcagaaatag
    16261 tggatatttg gtctataaat aacattgggc tatttatctt attctttaaa tcttcatata
    16321 taagattaag agagcccata tcaaataata caattaaacc attctttatc ctatctccct
    16381 ctagatatct agacatttgt tttgatacat cagcaatagt agcatctata ggcatatcaa
    16441 tagctgtaaa tatattttta cccaacatcc gattacatac attcgccata ctactggcag
    16501 tagcatatcc atgtgctata atagtcgcat taacttgatt aaatttcatt cttataattg
    16561 ttgatttaat aaatataatc attatgattg tatctacatt attaagatac atatccaatc
    16621 tattcgttat taatgttgat attttactta ttatagagta ttctttgtaa ctctcttttt
    16681 ttaaatatat tagtaattta ttttctaaag ctttatactc atcagacaat tgataagatt
    16741 cgtccttcaa ataaagataa gacgaaaatg ccaatacatt attcccatca tattttatat
    16801 tgttattaac ttctaaattt ctaaatatat cctgcattat cgatattata agctccataa
    16861 gtacgttatt attttcatca ttatttgaaa agactaaatt gtccattagt ctaatcatta
    16921 gattagaact ctttttacta aatttttcct ttgttatttc atttttttct agtctatcaa
    16981 agtaatctaa acattttatg aaactttgat agattatatt tcttttattt cttccatttg
    17041 acatttcaat actaaattgt ttaggagaaa agataatgct tttgctttta ccactttgga
    17101 attgaaatat atctacattt tctatataaa gatttctagg aaggtcttga agagtaacaa
    17161 aaatatttcc atttcttctt tgattttttg cataagctcg tccacatgca tattttatta
    17221 catttttaca ttcacctata ttccccttga attttatctt taaaatcgca tttaatgtaa
    17281 tttgagttat ctctataggc aaaccaagta ccttactttc ttcataaata aaatgttcta
    17341 caatctttct tttttcaata gtccctcttt cttctatatt gggaacataa atatttatgg
    17401 gtattcttct taaaaaggtt tgtaaaaatt cagatagact ttctgttgta gcaaatatta
    17461 atcttaccct agcttttcta attactccat tctctccaat cctagaaaac gtacctgtat
    17521 ccataaatgt aaataacttt tcctgacctt cactatttaa tctatggact tcatctaaaa
    17581 ataaaacacc accatctgat tgttctatta gacctgctct agattcattt gctccagtat
    17641 atgctccttt tacatgccca aataatagac tagacaataa ctccaaatta ttataatact
    17701 gcgcacagtt taacacattg tatggagaat ttttttctaa tacattactg cttacactat
    17761 atttgtatat tatcttggct aaaaaagttt ttcctactcc actttcacct gtcagcatta
    17821 ttgtaagtcc gccatttgga taaagtacag cggtttttat ttgttcgatt acttctttta
    17881 agctattatc atatcctatg atactattaa acacagtctc ttgctttaat aatttacact
    17941 catttttaag ttcatctaaa ttttcataaa tacttttttt tgtaggtcct actacatcat
    18001 ttattttatt tttaggtaaa aaaataacag gtctactgtt tatctttata agattatcag
    18061 attttactaa atcatttaat aatctacttg attgagttct acttaaatta aactttttta
    18121 ctatatcttc tgttattatg ccactttctt ttttaagttc atcaaagctg ttaaataata
    18181 cacattcatt tacaaaactt aaaatttcat ccctcatgtt caacctccaa tacaaatatc
    18241 aataatacta taatattagt atatcattta tatatttatg aaaaagataa ttatattcct
    18301 atcttgattc tcttttgtaa ggttgtaaaa tcaaactttt tataagtata aaatctagat
    18361 tttttaaca
//
LOCUS       DAEMGG010000075        18348 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEMGG010000075 DAEMGG010000000
VERSION     DAEMGG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.075"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(484..1551)
                     /gene="rsgA"
                     /locus_tag="KPK24_002396"
     CDS             complement(484..1551)
                     /gene="rsgA"
                     /locus_tag="KPK24_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG2300064.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(1548..1949)
                     /locus_tag="KPK24_002397"
     CDS             complement(1548..1949)
                     /locus_tag="KPK24_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG2300065.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      2746..2842
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2896..3072
                     /locus_tag="KPK24_002398"
     CDS             2896..3072
                     /locus_tag="KPK24_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300066.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            3482..4120
                     /locus_tag="KPK24_002399"
     CDS             3482..4120
                     /locus_tag="KPK24_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300067.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(4609..6369)
                     /locus_tag="KPK24_002400"
     CDS             complement(4609..6369)
                     /locus_tag="KPK24_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2300068.1"
                     /translation="MQEVEVIKAKNVLARQARGKAIERLRVATYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSKYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKTQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(6432..6575)
                     /locus_tag="KPK24_002401"
     CDS             complement(6432..6575)
                     /locus_tag="KPK24_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2300069.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            complement(6894..7133)
                     /locus_tag="KPK24_002402"
     CDS             complement(6894..7133)
                     /locus_tag="KPK24_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300070.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            complement(7138..7554)
                     /locus_tag="KPK24_002403"
     CDS             complement(7138..7554)
                     /locus_tag="KPK24_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2300071.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGAMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            complement(7551..7850)
                     /locus_tag="KPK24_002404"
     CDS             complement(7551..7850)
                     /locus_tag="KPK24_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2300072.1"
                     /translation="MKHLHIQQLSTIGSSLKKHLNSTEHNAPICGFLQHITGIICLAQ
                     DQYVCIALVLFYCIKLFQKIFAVGYQIPPFMVSVSAKRGRSLFALATFAGKGVRL"
     gene            complement(8901..9068)
                     /locus_tag="KPK24_002405"
     CDS             complement(8901..9068)
                     /locus_tag="KPK24_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300073.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            complement(9081..11846)
                     /gene="mgtA"
                     /locus_tag="KPK24_002406"
     CDS             complement(9081..11846)
                     /gene="mgtA"
                     /locus_tag="KPK24_002406"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBG2300074.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            complement(12012..12323)
                     /locus_tag="KPK24_002407"
     CDS             complement(12012..12323)
                     /locus_tag="KPK24_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG2300075.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            complement(12570..13247)
                     /locus_tag="KPK24_002408"
     CDS             complement(12570..13247)
                     /locus_tag="KPK24_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG2300076.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            complement(13385..13888)
                     /locus_tag="KPK24_002409"
     CDS             complement(13385..13888)
                     /locus_tag="KPK24_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2300077.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            complement(13904..16567)
                     /gene="mgtA"
                     /locus_tag="KPK24_002410"
     CDS             complement(13904..16567)
                     /gene="mgtA"
                     /locus_tag="KPK24_002410"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBG2300078.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            complement(16660..16869)
                     /locus_tag="KPK24_002411"
     CDS             complement(16660..16869)
                     /locus_tag="KPK24_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300079.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRERYEKMLAVSIRAPNLEGM
                     VENFDKDLSRPNETDLIELKNAISK"
     gene            16819..17154
                     /locus_tag="KPK24_002412"
     CDS             16819..17154
                     /locus_tag="KPK24_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300080.1"
                     /translation="MTHMSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVA
                     VWKPSVKTIQLVCMEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYS
                     CDLQQNKTT"
     gene            complement(17353..18255)
                     /locus_tag="KPK24_002413"
     CDS             complement(17353..18255)
                     /locus_tag="KPK24_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2300081.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKESNWKIIG"
BASE COUNT         5909 a         3738 c         2850 g         5851 t
ORIGIN      
        1 attatatttt atcatcaaaa gtatacattt acaaatatta gttaatagtt ttataggttc
       61 gagcattatt ttcaacaaaa aatttttatt ataaattaaa acaataattt ttataaatat
      121 attatttgta ttttataatt catatatttt tactttataa attagcaata ttgcagtttt
      181 gctcttaaga aacatctcta tcatacataa gcatatactc tattatgtat tagtataaat
      241 atgccttttc ttatcttaca taagacctct ttataaagga gcctatcgta acaaatatgt
      301 atacattatc acatgtatat atcatcatat aaaaaatcaa actttattaa aaaaattaat
      361 aaattgcttt aaataatacc acaaaccaaa tttgccatca aaatcacatt aaagtaacaa
      421 attgatggca aatttaatat attaatagta tatagactca tagaatgaac aaaaaaacta
      481 cacttatctt ctaatcttct tatttatttt tgagatattt ttaaatttct tttctttttt
      541 tgctaagtat tcgcttttat tgtctgcaaa tactgcttca gttttaagcc tacgatagga
      601 ttcccatctt ttaggcgaaa gttcttgatg agcaatagct tcccagatag cacagcctgg
      661 ttctgaggtg tgagtacaat tacgaaaatg acattttccg aaataagctt ctatatctgc
      721 aaaactttta tctaaacctt ctgaaacatc ccacattccc aactctctca tacctggtgt
      781 gtcaataaca ataccaccac ctgaaattaa aaatagctct cgtctagtcg tggcatgtct
      841 tcctttatca tcatctatgc gaatttcctt tgtatttaaa aattctccac ccatcagaca
      901 attaattaat gaagattttc caactcctga agaaccaagt aaggcaacag tacagcctgg
      961 tttaatatac ttagaaatct gtttataacc atctggctct atagatgagg taacaactac
     1021 atctacaccc aaggctatag aattaactac ttgtaattta cgatgcacat tgtcacataa
     1081 gtctgattta gtaagtacta ctactgggat agctccactt tcccatgtta aagacagata
     1141 cctctccaat ctatgaatgt taaaatcctt attcagtgac atgcacaaaa aaacaatgtc
     1201 aatattagct gcaaccacct gctcctcatt tacctttcca gcagccttac gcacaaacaa
     1261 gctttttcta tgaagcacat gatgaataat agcattacca tttttattat tgcaacgatc
     1321 caacataacg aaatcaccaa ctgtgggaaa atcagattgt atagtagcct caaaacgcag
     1381 ctttcctgaa acctctgcaa acagtacacc atttacacat acagcttgat aaaagttctt
     1441 ttcctgcgag ataatccgag caaggtataa atcatgatat tccatacttt gagtaataaa
     1501 atttctattt agaccatagt cttgtaaatt gatttgtttc ataccattca ttctgtttcc
     1561 tccttttcca acttttcaat gattctttct agtgggaaag ggggctgtgc cagtggtttt
     1621 aatgcaattg acattaattt cactgaatta tcatcagctg caatacgatt agaactaaga
     1681 tgtccacagc gtgtacaacg atgaataatt gcccactcac cattttttct cacccacact
     1741 ccaataggtt ccatattagc atcacaatct gcagcacggt caccaggaat gatatctaca
     1801 tgaagactat gtaaacaatg tggacaatga tttctatgtt gtgtaccagc tccttcagga
     1861 actacaaggc aaccacaatt tttgcaaata aagttatcca tacaggaatt ttcctgataa
     1921 taagctcgtt ttctattttt ataattcatt tttatttcct ccgtatcgtg tattttctac
     1981 aaaaaaagtc cacaggtatt tatatctgcc tttttatgtg gtatacatat ttacctacac
     2041 aaaaacagaa aacccctgtg gactagaaaa tttagtatct tctatttcaa cataattata
     2101 ttcaaaataa tactgtgtaa aattaataaa agtgtagcag attaaagccc actacaccac
     2161 aatttaacaa agtaatatgt aatattatta tttgttgtct aatgttagtt atctataaag
     2221 gcagagtaag acacaacaat caaatgtatc aaaaatacac ttaaaaattc tgattaaaca
     2281 gaacccatat gcgtctccaa ctatttattt gttggttacc attatatata actctgacat
     2341 caggcaagac actccttcac taataattat tattgaaatt ttatcataaa atataagtat
     2401 gttcaagtat aatttaatat attcatatat ttatgtttgt ctcattttct tattctataa
     2461 aatctagtgg tattttgaaa tttaatgttc acctagtatt aataataaaa attaactgct
     2521 attatttgtt ttctcatttt atgcctgttg ctcaatttat ctcacttaaa ttaataacaa
     2581 ctgtcttttc atacaaaacc tacaatcaag tttttccata tattctacat gcatagtcta
     2641 ttttttttga tataaaataa cgttcatatt tgtaaattta tttaattttc aaataagtat
     2701 attgaataat aagcaattct attgtattat tgtaattgta gaataaaact aaatcggcaa
     2761 aactagagaa atttagtgac gcaaagctat agggactaac gcttataaaa taaactatga
     2821 gttatgtcag ccagttgcca aaaagagata ttctttttgt ttttatgaaa gttttttagg
     2881 gggataataa tatatatgtt tagagataaa atggatagat gtacacatat gttgactgct
     2941 tatattggta gttcatatga ttattgtgat tttatagata cacaactaga tgattttata
     3001 ctagagtacg gagaaaatgt ggtagaatct tgtttgcatc aagtgatggt attagtaagt
     3061 aagtataatt aaatagattt ggttttctag agaacccttt ttagttttat ctactttaaa
     3121 tagccttatg ttagaaagct aattaagata gatttctagt attcataatt ttatttttta
     3181 tttaaaatac atataataaa gatgtaaatt tgtatcaaat aataaatatg agaacttaaa
     3241 ttctcttatc tatgtgaaaa taaacatctc ttaaaatgaa attctgtaaa tataaatgat
     3301 tttttagttt gaaggattag attaacaaaa ttataaattt gcaaataagc tatattaaaa
     3361 aatataataa ttttttatat aacttattct tttataactt ttaattttaa aagaggagaa
     3421 tgtatataag agtattcaat gtactagaaa aaatcaaaag aaatattaga agaaatggat
     3481 attgagtaat caaagaaaag attttgatat tacaaatgat atgtttcaag aaagcattcc
     3541 tatcacaaca aaaattattc ttgaagacat gccttcaaat gatgaattaa atcatgtttt
     3601 ttcaaaaggt tgtgaacgaa aaatgaaaaa gagaaaaatt gtattaatca cactactatt
     3661 aataggtgtt ttgttattag gaagtatcct atataatctt tttttaggaa aaacagctaa
     3721 tatttcaatg ctaaaggaat cttggaattt tgatatacca atacctaaca aagaaattga
     3781 agtatttgat actcaagata gtattaatgg agatggacaa agttatttta ttcaaggatt
     3841 ttctgaaaaa aatttcaaaa aagtttttaa tttaaaaggt ggtatagttg taagcaaaga
     3901 taatattaat gaaatagaaa aatatattga taaatttaaa agagattctg ttaacattaa
     3961 taaaagtaat aaaaataaaa ttgaagaaga ttttaaaaaa tataagttag aagttaaaaa
     4021 agatgataag tatatatata agagaaatta tgagaactac gtagttttaa ttataaaaaa
     4081 agatgaacaa aaattataca gtttaatatg gaatcagtag gtacatatgt attattaaga
     4141 attataaata ataaaaataa attttagtca atatacaaaa accttttaaa ttgtctggaa
     4201 atcacctctt catttgttat aaaaacatac aacaaatgaa gaggtgattt tatggtttta
     4261 aaaaaaataa ttataagtgg atatgtgctt tcacatgttt atcaacattt gtaatgccta
     4321 tatttgctaa tgaattataa aaatattaaa taatattcaa ctatagatta tgtaaattac
     4381 tctatgaaaa aaatgagttg tataaaatct ggctactgta gattgactat ttcttttaaa
     4441 attacaatta taataagttt tatagaatta aaaataaaac tttagagatt tttgataaca
     4501 ttgtagacta aatttgccac cgaaatcgct ttacagtggc aaatcggtgg caaatacaat
     4561 atattattag tatataatct atataaacat tactattgtt tgattatctt agctacagtt
     4621 tccacatgtg tcgttactac tatcggaaca cattttatca acctcgttgc tgtcatgtga
     4681 acacaattca ttagtcctat gccgtcctct tgcatttttc ctttgtgttt taaatttagc
     4741 accatctacg ctattcttca agcctgtttt ataaacaaag gtcaactgtg cagggtcaac
     4801 atttccgttt tcatcaagtc cacctacaat tactttttca acaatgcttt caaacacata
     4861 gcggtcaaat ttatcaagaa cctcattctg ttccagagtt ttcttaaact ctttcaaacg
     4921 tttttttatg tctttctcat tatcagaagt ttcttgtaac ttttgtcttt cttcaacaag
     4981 ctgttcttgt ttacttacta aatcagcata tttactttcg taggtttcct tatcaataat
     5041 ttcttccaga cgcatatcca caagtttact ttttttcttc tctaatgcgt ctatctcctt
     5101 ttccgctttt gcaagttgtt ttgacaccac actactacta agagtatcgt ccattctctg
     5161 caaaaattct tccagaacat ctttattatc atcacataat aaacgataac tttctacaaa
     5221 tgcttcttca atagctgttt ctggaatacc tttgctatgt ttacaaaatt tctttccttt
     5281 cttcgtagcc gttacacatt gccatataac cttgctgtat tttgagccac tgtgccaatt
     5341 acgtcttgtt aaagtaccac cacaaaagcc acattctagc atacagctaa atgcgtattt
     5401 cctgctatac ttctcacgct taccgctgcc taccgttgtt cggttcttat ttctccgatt
     5461 aagaatttcc tgtgcttttt caaaaacttc ttcactaata atcggctcat ggtgatcttt
     5521 catataaaat ttatcttctt cacccatgtt ttcaaggcga cgtttggaaa tcggatcaac
     5581 agtaaaagtt ttgcctaaga gtatatcacc cttgtatttc tcattcttaa taattccaat
     5641 aaccgtagat ggtgcccatg tggaactccc atacttcgtt ttataaccta gactttcaag
     5701 ctcctgtcca ataacagaac caccagcacc atcaacataa cggttgaaaa tatatctgac
     5761 aatctctgct tcttcttgat ttacagttat tgtcttatcc tctgggtgat aatcatatcc
     5821 aatacaacct tgaaaaccta ccagctcgcc gcgcttcatt ttcattttca accctttttt
     5881 tacattggct gaaatgttct cgacttcctg ctgtgcaaca gaactaagaa caacaagcaa
     5941 taactctcca tccattgtaa gtgtattgat tttttcatct tcaaaataaa cggcgatatt
     6001 tctctctttt agcatacgaa catatttcaa tgtgtctaaa gtatttctgg caaatcttga
     6061 tatggatttt gtaaatacca tgtcaatttc accattcata cagtcattta tcatacgctg
     6121 aaaatcttct cgttttgtta cttgtgtccc cgtaattgct tcatcagcat aaatgtcagc
     6181 taacacccat tccttgtttt tcttaatcat gtcagtataa tactgaactt gtgatttata
     6241 actgtttaac tgatcttcgc tatctgtact tacacggcaa tatgtagcta cacgcaaacg
     6301 ctcaatggct tttccacggg cttgtcttgc caaaacattt tttgctttta ttacttcaac
     6361 ttcctgcata gtacacctcc ttattttgtg ttcctttcat attgttattg tactacttta
     6421 aaaaacacaa ttcaagcagt tatgtcagaa agcaccttat agtctttcat cagcctttgt
     6481 tttacaagag tgtattcttt ttcagaaatc agtttacggg caagcagttg ttttaacata
     6541 gcaatttgca tactatacct aattatatta ttcattaaaa ccctccttgc ttttttattt
     6601 atggaagtaa tcagacggct tcggcagctc cactggactt tcaccattcc cattctgatg
     6661 ggtgaaccgt gtcatacggg tgtatcatta ttcagatata cgagtcatgg cagcaacctt
     6721 tccaaagatc gcttgcggtt cactggcata atcgctcgct gctttcccca tttcagtggt
     6781 cgtggcgttc cagtccgctg gctcgtcgta tatcaaacgt atctgattac tgtattcact
     6841 tgtcaaagaa cggaaaagag ttaaaatgag gtggtagata ctctctgtta tatttacttc
     6901 actttgactt caaaccctaa gattacttta atcaacgctg cccgtatcct gcctttcagt
     6961 tccatatcaa gcacgatata catattcccg tgttcgtcat agaacgggcg taggctggct
     7021 tttgatatgt aagcgtcata atggttcaaa atcttttcaa ttgccacctc gttaccgtca
     7081 gctgcagaac tgatggtcga gaatgatgga cacttttttg tagacttcat catgttttta
     7141 ttcctcctcg tacatatcct taataagctt taatgtgtgc agcctgtttc tacatacgga
     7201 aaatcgttcc atttccataa cctctgcaat ttcagcgtcg gtcatttcaa gaaagtagga
     7261 cataagcaga atgttacgtc ggctttctgt taattctttg atagcttcac ataagcgttc
     7321 atcatagata tggacttctg aaccaaatac atcaaatgac gtaaactcaa cgcaatattc
     7381 gtccatggca ccgatctggt ttaactccat ttcagataaa tcacagaacg gcgtttcacg
     7441 tcttgcacgt ctgccaagct ctcggtcata gttctttaca gttcgtccta caaccttgcg
     7501 agctagacaa tcaaattgaa gtctgatagc gccctcgaaa gaagatggtt tcataatctc
     7561 accccctttc cagcgaaagt tgctaaagcg aaaagacttc tacctctttt cgcactaaca
     7621 ctcaccatga aaggtgggat ttgataccca accgcaaaaa ttttttgaaa aagttttatg
     7681 cagtaaaaaa gcacaagggc aatacagacg tactgatctt gtgctaaaca aattattcct
     7741 gttatatgct gtaaaaaacc acatatagga gcattatgtt ccgtagaatt taaatgtttt
     7801 tttaatgaac taccgatggt cgaaagttgt tgaatatgta agtgtttcat acggctaccc
     7861 ctagagccga taaaacaaga ccatccagta atttgtccaa cgttaggcaa aaataaaacg
     7921 gcggctatat aaccgtcgtc ttattcaaaa gttcaaaaag aactaaacta aaccctctaa
     7981 agcagtatgt atttataatc agtcttgcaa aataacaaag tagaattgaa atataaggta
     8041 gcaatccttg tgcatctaat aaacttatag gggttacagc agaccacgga ataatatttt
     8101 gtatactttc agatcaatat agtccaacaa aaccaattcc cgatatttag taactaaaaa
     8161 gtaaactaga gtagaaaaca tttctttgca aaacacaaat aataagtaat ggactcattg
     8221 acaatagatt gagaaatcca gttttttaga cttagataga ttatctcact tgtaaatgat
     8281 ttaagttctt tgacttcata tatagcggta taatctgtca acgtgatcat tacaaaccat
     8341 aatgcctata taacatactt tccaagcaaa aactttattc tattagttgg ggaatacaaa
     8401 atatttatca ttgtgcgttc aacatattct ttttgtataa aaatgtaatt acaaaacacc
     8461 actcaatata agatagataa taatttgaat aggaattatc aatctacgta accattcatt
     8521 acctgtcatt gttgttacaa tagatttggg attctgtttc cattacataa tttttttatc
     8581 cttctttcag atatctcctc attttatcga agcttcttct acagacatac attccataat
     8641 aaaatctacc tttgaatatt atttcacgat ttcgttatag acgcgtaaac aaacaataaa
     8701 aaacgaaatt tcttttaagt accataattt ttatatcgaa attacaatta tccacttata
     8761 tacagcattt ataggtaatt caatgataaa aaaacacata ctttactact aaattgccac
     8821 aattgaatgc gccttgatat gatagggaga ttccacaata aaaaactgtg catctccctt
     8881 gattcattca tactgatatc ttatttataa gactcttttg tgtctttttt tggtttcatg
     8941 gaccaaaata ctacattcat tacaataaca atcagcaaca caactgccat agccacccaa
     9001 aagccgatat taagaccaag ccattcggta gttccaaaaa gggtcatcca aagttctttc
     9061 cagttcatca ttccacctcc ttaaagcagt tctccgaaat gacggacata ggcttttttt
     9121 aaacttgttg ccaaagtcat atacagcaga atacatggaa tcagataagc aaagtatgct
     9181 gcaggcaatg caacaaatcc aagcatagtt ccaaacacag taaacggaat aatggtcaga
     9241 actataattc cagtcattgt tagcaaagtc accggagctg atgcacgact ctgaacaaaa
     9301 ggtagctttg gagtacgaat catgtgaatg actaatgtct ggctccacat agattccacg
     9361 aaccaccctg cttggaacat cccgatatac tgtgcctgaa gcattgccag ttctgcaccg
     9421 ctgaaatgag caggcaaatc attaaataat acaccattcg atacgaacaa tggacaaaat
     9481 acaaagtaca taaaaatata ggttgtaaaa tcaaagatag aactggttgg tccaatccag
     9541 atcataaaac ttccaacact ggaagcgtcc catttacgag gtttggtaat aaactcctca
     9601 tccacattat cccaaggaat tgcagtacag gataaatcat aaattaagtt tagaaatata
     9661 agatgaacac tcatcattgg taagaaaggt agcaaagcag atgcagcaag cactgagaac
     9721 atattgccga agttggatga agcagtcatt ttaatgtact tgatcatgtt ggcatatgtc
     9781 tttcgtccct caataatacc ctgttccaaa accatcagat ctttttccaa tagaataatg
     9841 tcagcagatt cttttgccac gtccacggcg gtatccaccg aaataccaat atcggcggct
     9901 ttcatagctg cagcatcatt aataccgtca cccatgaaac cgaccgtatg accattttca
     9961 cggagaacag aaaccacacg ggctttctga tcaggggtta gctttgcaaa tacatctgta
    10021 gactctgcca ctcttgcaag ctctgcatcg cccatttgtt ccaaatcaga accgagaagc
    10081 atattgcgaa cctctaatcc cacctgcttg cagatagttc gagtcacttt gtcgttgtca
    10141 cctgtcaaaa ttttggttat aacaccatgg tctttcagag ctttaatagc atccgcagta
    10201 gattcttttg gaggatcaag gaatgccaga tatccaatca gtaccatatc acactcatct
    10261 ttaacaccaa atgcgccgat tggggaagga ttactcttct gtgcaatagc tagtacgcgg
    10321 aatcccttat cgttaagatt gtccactgtc tccagaattc tgtaacgaac gtcatcagtc
    10381 agaggctgca ctcctccatt acactctgca aacgcacaaa tagagagcat ttcttccaca
    10441 gcaccttttg ttaccatctg ggtcttaccc ttattatcct gtaccactgt agtcagacga
    10501 cgacgtgtga agtcaaaagg aatctcatct actttcacat agttttcaga caggtcaatg
    10561 agacgcgaat ctgcggcttc ctcttcctcc gtcttatgga taattgccaa atccatcagg
    10621 tttttatagc cagtctggaa gtaactattg aggtaagcgt gacgcagtac tcgcgtatcg
    10681 tcctcgccat ttacattcag atggtattcc agcacaacct tatcctgggt cagcgtgcca
    10741 gtcttgtcag tacagagaat atcaatagca ccgaagttct gaatggaatt gaggttttta
    10801 acgattgtct gcttcttgct catggaaaca gcgcctttcg caagacaggt tgttacaatc
    10861 atcggcaaca tttctggtgt caaaccaaca gcaatagaaa cgccgaataa gaaggccgcc
    10921 aaccaatcgc ccttggtgat accattgaca aagaacacca gcggaaccat aatgagcata
    10981 aaacgaatca atacccaaga aacggcattg acacccttgg taaagcttgt ttcaaccgct
    11041 tctccagcaa ctgcggacgc catagagcca aacagagtat gatcgccaac acaaacaaca
    11101 acagctgttg cgctaccaga aattacatta ctccccataa aagcaatgtt tgtgtaatca
    11161 gtcacgcttt ctttctgagc acttacattg ggtgtttttt caataggttc actctcacca
    11221 gtaagacttg cctgactgac aaacaaatcc ttcgcatcca aaatacgaac atccgccgga
    11281 atcatatctc cagcagaaag atgcacaata tctcccacca ccaaatcatc cataggaagt
    11341 tctttttttt catagtttct acgagtaact gtacaagtgg tcgtaatcat ggctaacaat
    11401 ttctctgctg catttccgct tctggattcc tgtacaaaac gaagcgtacc ggaaatgaat
    11461 accattgtta aaatgattac aacagtcagg gaatcaaagt cctctggcac actgccaaac
    11521 agagaaaaat acggaaaaat catatctgta attgtggata ctaaagccag acaaaataaa
    11581 atggcggtaa atggattgat aaatgcgcca accagtcgtt ttgccagaga tttctttttt
    11641 tcatgagtga ctttattggt accatattta gcacgactta cggatacttc atctacttct
    11701 aagccacgaa gtgtggtatg aagttttttt aaaacttctt taataggatt ggttgccgca
    11761 aattgaatgc ggcggttctg ttcgtcacgg atgactgctt tttctacagt ctgacgaata
    11821 gccatacgat tttctttttt gttcattggt cttacctcct tgaattttca taggggcaag
    11881 gaatattata tgagcgacac ggaatctcca aattctcata taatattctc tgccttcgtg
    11941 gactgacatc agagtccatg tctttcacct cacaatctta aaaaaatatc tatgtgaaac
    12001 ctcaatgtgg attattggtt gcgatagcgg acactttttt cgatatacca gacatcaaag
    12061 ccggcaacaa tcagccatgc gattattgca aatacaaacc agtggtttga aaatcccggc
    12121 aaaaatcctt tcattaagaa tataatccca tttaacgcaa tcatttctcc aacattacga
    12181 caaagcggga caatattaat tttatctttc tcctcctgcg gcatattttt ccacagggaa
    12241 agatgacgat atccttttcc aaaggccaac caaaagcctg caagagtaaa taaaattcca
    12301 aaaaagatac atgtaaaatc catcgtttgt tcctcctttc aatgcttaaa cctgtttctt
    12361 agttttgtca gcttcagaag tttaatgaag agcacaataa tagtaacagc aagaataaac
    12421 cagatcacaa tactcacctg ataccatatc ttaaagatta ccattctttg ttcaaagaaa
    12481 ttccaaccca aatagctaca taagattgta aatacagggt agaaagcaaa aagaattgct
    12541 cgtttcaaat cttcgatttc tctcctcact cacaacacct cccaaccagc actaatcact
    12601 tccggaatta ccaatacctg cccaacgatt ccttctaaaa tattgttttt ggattttccg
    12661 acagaacagt attctgcgac aatctcaacc ttgtcaccaa ccacatcacc gctttccaga
    12721 ttgttcaaaa agagcgtctt acaggaattg ctgttaatca gcaataagcg tatatcctgc
    12781 tctgcctttt cctgacatgt aaccgaaata cgatactgct tttcagattc atcactggaa
    12841 gtaattgggt gaaactttct tgcaaaggga cgtagaatca ggttggaacc aattaaaata
    12901 gtagcggcgg tgatcgccat tgcatacatt cccgtacttg caagaattcc aatcgctgca
    12961 gtacaccata aggtggcagc ggtattcatt ccacgtactg tccctctgtc cttgaaaata
    13021 acaccactgc ataaaaaacc aacaccggat ataatactgc ttccgacacg gaatacctga
    13081 tccgaaccgt agagcatagg aaacaaggta aagaaacttg tacccatgca gattaacaca
    13141 ttgatacgaa tacctgcagg atgaccagtc aattgccgtt ctaatccaat aagaaatcca
    13201 agtgttaagg agagggcaat tctcaaaata aaatctgtgt aaaacatatg aaacacctcc
    13261 ctttaactac taacagtata taaaaaatcc aaatacaatc ctatggctaa agtcttgcga
    13321 ttttcttgcg atttggtaag aaaatagacg gactgaaacc gaaaatggtc ttagtccgtc
    13381 tttgtcatat ctgatcatta aatttgtatc caatacccca gatggtcaaa atatatgtgg
    13441 gacaatccgg agcaggttca atcttttttc gcaacttccg gataaaagcc ataatgttgc
    13501 tgttatccag aatataatct tcctcccata cggcacgata aatctgttcc tttgtaaaaa
    13561 cttcaccttt gttctggaca agaaagtaca aaatatcaaa ttcctttgga gtcaggttga
    13621 cttcagaacc ttttatcagt acttttctgc ctcttggata aatttccaga tcccctgcca
    13681 ggattctatc tgacagagta cccactggcc aatcgctttc ttctgatgct cctaacaata
    13741 acgaagatac ctctccacga atcatctcct gaataccaga aagtaaatcc ttcgcacaat
    13801 cactgtccgg tggatctctt agtagttttt ctaatagcca gatttcaaga gccgaatcca
    13861 tcggaatata actaatattc tttttcatca tgaatgcctc ctcttaaatc aactcttgat
    13921 attttttctt ataaaaatat ttggctactg aattcagcag catatagaga gataccacca
    13981 gcagtaaaaa tacgaaatac caaagaggta atttcgtcag tccaaacaac gacgcaccgc
    14041 cgacaaatgt aagggaagta aaagcaataa tccccactag agttatagaa atgaccggtg
    14101 cagatgccct gctctggaaa aaaggaatct tggaagttcg cagaaaatgt aaaatcaaaa
    14161 cctgtgtcca catagattcc aaaaaccatc cagtctgaaa cagtgctaca tactgaagtt
    14221 gcagggaagg atctgtaagc tgcagatagg ttgcccctcc acatagcatc ggacatagga
    14281 aataatacaa aaataggaag gtgacaatat caaacagaga actgatcggt ccgaaagaca
    14341 gcataaactg tctcagtgtt ttgccggacc agtctcttgg ggacagcatc tcttcctcat
    14401 ctacattgtc ccaaggaagc acaatacaca acgtatcata cagcagattc aaaagcagaa
    14461 tctgaattga agtcataggc aggaacggta aaaaggcact ggcacaaatg atggaaaaaa
    14521 tatttccaaa gttagagcta gcggtgattt taatatattt cagcatattg gtaaaagttt
    14581 ttcgcccctc cattacacct tgctccaaaa cattcaagtc cttttgcagc aaaaccacat
    14641 cggcagcatc ttttgctgcg tccactgccg tatctacaga gattcccaca tttgcttcat
    14701 tcagtgccgg aatgtcgttg actccatcac caagaaatcc cacggaatga ccattatcct
    14761 gcatagcaga gacaagacgc accttatgtc ccggagtaag ctctgcaaaa acatgagtat
    14821 gttccacagc ctttttaagt tcacagtctg tcatttcatc cagttgagta ccagttaaga
    14881 tacgctctgt ataaataccc acccggcggc aaacagacac agcaatcgct gcctggtccc
    14941 ctgtcagaat cttagggata acttttaatc ttttgagtgc agttacagac tcctttgctg
    15001 tctgctttgg tgcatcaaag aaggatagat agccaactag agtcatgccc cattcgtcat
    15061 caggagttat tttcttttgc gctcccacat tttttcgtgc caccgcaatg accttcattc
    15121 cgtcctgtaa catttcacct actacagaag atacactctg tctcgcatcc tgccccatgg
    15181 ggagaagttt tccacgatat tctacatggt tgcagcgtga gaatatatgt tcaatatcac
    15241 ctttcataat aagctgacta ttcccattgc tatcctgtac aagtgtactg acaaacttac
    15301 gagcatagtc aaagggaatt tcatcagctt tttggaattt tgtcagaaga tttgcgtaat
    15361 gggcttcccg ccctggcatg gtattgcagg caagaatggc attatcaatg gtattgcaga
    15421 caccagaatg atagcagcta ttcagataag caagatcaag aacctctgca ttttcattcc
    15481 caaaaatatc catataatat tccagcagga tgctttcatt ggtaagtgtt cctgttttat
    15541 ccatacaaag cacatccatg ctaccaaagc tctgcatggc attaatatcc ttgataatgg
    15601 tctgcttttt gctcatagca agacttcctt tggcaaggca ggctgtaata accataggaa
    15661 gcatttccgg catcagacct acagcaactg acagggcgaa agcaaaagat tccatccatt
    15721 ttccatcagt aatccccagt aatataaata caatgggaac taatatcgcc ataaaacgta
    15781 tcatcaccca ggcgatggaa tcggctcctt ttttaaagga atttttatca tcagaatccg
    15841 gtttggtaaa tcctccatac agggtatctt tgcctactgc cagtacaatg ccttcccctt
    15901 ttccactgat gactgtggtt gccataaaag cgaggttttc aagctgagtc agtgtatcct
    15961 gctctccgta actgagtttt cggcagctct tttcaatgat ggcgctctcg ccagtaatcg
    16021 cagcctgaga gataaagaga tccgtaatct tcgtcaatct gatatccgcc ggaacacgat
    16081 cacctgcaga gaatagtaca atatccccta ccactaattc ttcgccagga atctctatta
    16141 actttccatc acgcctcact gtgacgcttt catgaactag gcgatcaagc tgagcagcag
    16201 cgctctttgc tcgcagctcc tgaatcagac gaattacgcc actaattacg atcatggaaa
    16261 agatgataat ggcggtagta gcatttctcg caaaattgga tgctacgaaa acatccgtaa
    16321 caagagaaac aatgcccaga acaaaaagaa tcacatgaaa cgggttaata aatgcccgtc
    16381 tcaaccgttg catcgtcgta tcatttttcc gcccattgaa gctgttcgct ccgtatttct
    16441 cccgcattga accgatttgc tcaatggata aaccatccgg agatgagcca atgtctcgat
    16501 aaatttctga tgttctacag tatgcgtatt ttttgatacg actatcaaat agtgttgctt
    16561 ttgacacgaa acatcccctc ctttataagt ataagtaata acattttaac acagtgttct
    16621 gaaaatgcta tatttatatt cttgcttttt tcttgcaatt tactttgata tggcattttt
    16681 cagctcgatc aaatccgtct cgttagggcg agataaatcc ttatcaaagt tttcaaccat
    16741 tccttctaaa tttggagcac ggatggatac ggcaagcatt ttctcatagc gctctcgcac
    16801 ataaataggc atttcccctt gacacatatg agtgctgaca aacttcttct tggttttcac
    16861 cttgaccatg tatcaaattt aaatgaacta cttagaaaag ttgctcataa taatatttgt
    16921 attatgagtg atagaatgac aaaagtggca gtatggaaac cgagtgtaaa aacaatacaa
    16981 ttagtttgta tggaatatct gaactacata attgaccact ctgtaattta tgaaaaaata
    17041 caatgcgcaa attggaacgg gacagaagaa accaatagta tttttaataa ttccccttca
    17101 ccattgatta ctcttatcta ttcttgcgat ttacaacaaa ataaaacaac ataaatttta
    17161 aatacgttga ctggcataat tcatatgtat agtaagtcta caattaagat gcgctttttc
    17221 taatccaaat atattaagaa aaaagttttg cgaagccatt aatactttgt ttaagatgtc
    17281 tttcataaat ataaagaata atgctcttaa caaaagcgtg caaattccta aagaaatgca
    17341 cgctttcaat aattatccta taatcttcca attgctctcc ttatgtagca ctaattcaaa
    17401 ctgcgatacc tgtgttgcct ttgtctgatt atcaatgaat ttcacggcaa ccttgacttt
    17461 cacattgtcc ccatcaaccg taaagatagg gtttaccaat tcagaataga ggtagtccct
    17521 gccgatagac tcaagcacat ttccagacac atagtaagca agctcctttt ctgtggctgt
    17581 tgggtatagc ttaaagaaag tttccagaaa tgcagtagcg tcatttatgg tatccgcgtc
    17641 tacacttgcg tctgcttctg gtgtctttgg ttcatagtct gatttctcga ttgctggtgc
    17701 aagggtaggg ttctgaataa ttaccatatt cccgccagcg tccacatgaa cttttacggt
    17761 ataggttgcc ttaaccgctg ttgtctgttc cccctctttt atctgctgat ctacttcgta
    17821 ggtagcggaa aaagtgtccg ttccagactg ttcaatgctc catacaatca catctgtaat
    17881 ggcagagctg gtcggtatat ccgttctaat ggtatccata ttcaagtcct gcaattcctt
    17941 tgtcagataa ccgctgattg cctgcgtcct tgcgtcaatc gcttctttgc tgttactcca
    18001 tgtgtaatag gactttgcaa aattcttcac gaaattttct atcccgttgg tgtcctgcaa
    18061 gcgaagctca atcacttctt tttcatgggt cgtgtgctgg tcaatagcgg taaaattctt
    18121 atatactcca aagctcacgc ttgcaataag caccacccac aatgcaatca cggttttctt
    18181 atgtgtgcct accttgacag tacgtacctt tttttctttg acattttctg tctgtttctt
    18241 attcttctta aacatatttt tcaagtcctt tctattgttt tactcgtcct gcaccgatta
    18301 agtgctgttg ccagtaacta ctacttaggt ctgtatatcc tatcgggt
//
LOCUS       DAEMGG010000076        18324 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEMGG010000076 DAEMGG010000000
VERSION     DAEMGG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.076"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..846
                     /locus_tag="KPK24_002414"
     CDS             <1..846
                     /locus_tag="KPK24_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2300082.1"
                     /translation="YINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRNNTMFEDVFINKFNLEKLIKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            1277..2437
                     /locus_tag="KPK24_002415"
     CDS             1277..2437
                     /locus_tag="KPK24_002415"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG2300083.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            2439..2837
                     /locus_tag="KPK24_002416"
     CDS             2439..2837
                     /locus_tag="KPK24_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300084.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            3099..3407
                     /locus_tag="KPK24_002417"
     CDS             3099..3407
                     /locus_tag="KPK24_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2300085.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            3496..4842
                     /locus_tag="KPK24_002418"
     CDS             3496..4842
                     /locus_tag="KPK24_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2300086.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPELLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            5075..6172
                     /locus_tag="KPK24_002419"
     CDS             5075..6172
                     /locus_tag="KPK24_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG2300087.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKRINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDIGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            6271..7185
                     /gene="murQ"
                     /locus_tag="KPK24_002420"
     CDS             6271..7185
                     /gene="murQ"
                     /locus_tag="KPK24_002420"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG2300088.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            7295..8215
                     /locus_tag="KPK24_002421"
     CDS             7295..8215
                     /locus_tag="KPK24_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG2300089.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            8232..9083
                     /locus_tag="KPK24_002422"
     CDS             8232..9083
                     /locus_tag="KPK24_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2300090.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            9695..10756
                     /locus_tag="KPK24_002423"
     CDS             9695..10756
                     /locus_tag="KPK24_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG2300091.1"
                     /translation="MKKIRKLTTKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(10872..11846)
                     /locus_tag="KPK24_002424"
     CDS             complement(10872..11846)
                     /locus_tag="KPK24_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300092.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            12302..13183
                     /locus_tag="KPK24_002425"
     CDS             12302..13183
                     /locus_tag="KPK24_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG2300093.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            13565..14368
                     /locus_tag="KPK24_002426"
     CDS             13565..14368
                     /locus_tag="KPK24_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300094.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            14380..15510
                     /locus_tag="KPK24_002427"
     CDS             14380..15510
                     /locus_tag="KPK24_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2300095.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            15514..16923
                     /locus_tag="KPK24_002428"
     CDS             15514..16923
                     /locus_tag="KPK24_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300096.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            17134..17643
                     /locus_tag="KPK24_002429"
     CDS             17134..17643
                     /locus_tag="KPK24_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG2300097.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         7329 a         1883 c         3207 g         5905 t
ORIGIN      
        1 tatataaatt ttagaaatag ttacacaaat ttatataatt gtatagaaaa tagtggtgaa
       61 atagacagtt atttagttga tggacaatct tttgaagaaa atctaataaa agatattata
      121 gaaaacttaa aaataaaaaa taatagtgac atagagactt ataaacaatc attaaaagaa
      181 ttggatgagt acatagctaa atgggttcat gaaataaaaa ttccaatatc ttctttaagt
      241 ataattacag ataggttaag tagcatagaa gatagtttgg atattaagaa tcaggttgca
      301 aagataaatt ttttagtaaa ctctattttg tatagtagta gaaacaacac tatgtttgaa
      361 gatgtattta taaataaatt taatttagaa aaattgataa aaatgtctat aaaaaataat
      421 tcatttttgc ttataaaaaa taatgtagaa gttagtttga atgaattaga aaatgatgtt
      481 tacacagatt ctaaatgcat gtcatatgtt ttagaccaaa ttattaataa tgctataaag
      541 tattcaaagg aagttggtaa gattgaattt aattcaaaaa agttagaaaa tggagtggta
      601 ttatctataa aagattttgg tataggtata aatgaggaag atatatctag ggtttttgac
      661 aagggattta cggggaaaaa tggaagaaat caattgtata agtctactgg aatggggatg
      721 tattttgtaa agaaaatgat agatagttta gggcatgaga ttgaggtatg ttcggaaaat
      781 ggttcatata caatttttaa tatctatttt tatgatatat cagattattt aagcctagat
      841 agttaggtta ttagactata aaatctatca tcatatacaa atcgacatat acaataagaa
      901 taaatttagt tcttgaaaat agtgaagatt taattagtgc cttaaatgat gctctatcta
      961 tattataaaa taataattat tatatatgaa aaacatatca gatatgtacc tggtatgttt
     1021 tttatttgta gtaactattt taatatggtt tttggagaga gaatatgttt tgtttgtgaa
     1081 aaaaaattac atggtattac attgacactg aaaataaatt aatgtaagat ttagctaagg
     1141 taaaaaacgt gtataagatt aactcaatta atttcataag agtaaattat cgtaattaaa
     1201 gatgaaataa gattaattct aaaattatta aacttaatac cttgatttgg ggaggaataa
     1261 ctataaactt aaaaggatga taactatgta tagtgtaggt ttaatgtcag gaacttcctt
     1321 agatggagtt gatgcagtac tagctgaaat tagtgggaat ggtagaaata ctaaagttaa
     1381 gcaaatagaa tttataactc tagaaattcc taaagatata aaagacgaaa taagaaagtg
     1441 ctgtatagaa gaggaatcga gtgttgattt aatctgtagc ttaaatttta agcttggata
     1501 tttatttagt aaagctgtaa aatcagtgtg tcataaagca aactttcata tagcaaattt
     1561 ggattttatc gcatctcatg gtcaaacaat ctttcatata ccaagaagtt ataataattt
     1621 tgtatcttca acgcttcaaa taggtgaacc agcagttata gcatatgaaa caaatacaaa
     1681 ggtaatatct aattttagag taatggatat agcagcaggt ggagaaggtg ctccattagt
     1741 accatatagt gaatttttat tatatagtga taacaataaa aatttagcac ttcaaaatat
     1801 tggtggaata ggaaatatta ctataatacc taaatcatgt aacatagatg atgtatttgc
     1861 atttgataca ggaccaggaa atatgataat agatggagta tgtcaaagat tatttaatag
     1921 aaagtatgat aaaaatggat actttgcatc aaaaggtaaa attaatgaag aaatgttaaa
     1981 agatttgatg agtcataagt atcttagtca agcgccacct aaaagcactg gaagagaggt
     2041 atttggacaa gtttatttgg ataacatgct taacaaatat aaacatgtag ataaatatga
     2101 tttgatagca acagtaacta tgtttacggc aaaagctata tattataatt ataaaaactt
     2161 catacttcca aaggttaatg tagatacttt attaattgga ggtggaggag ctcataatct
     2221 aactttaata ggatatataa aagagttatt attagaggtt gaagtattaa ctcaagatga
     2281 atatgggtat tcttctgatg caaaggaggc tttggcattt gtaatcttgg gcaatgaaac
     2341 attaaataat tctttttcta atgtaatatc tgcaacaggg gctaaaaata aagttatttt
     2401 agggaatata acacctaaac cttttggagg taaatgacat gtctaataat aaaaaaaaga
     2461 aaagaaagaa aaataataat tataaaaatg caaaaagtac aaaaagtaat aaaaatacta
     2521 agaaacaaat ggagaaaaat caaagaacag attataatat tccaactata agttttaaaa
     2581 gtggattaat actaatagtt tcatctcttg taagtactgt agtttttcct ttgttattat
     2641 ctatatttgg aatagataat agatttggag ttgttatagg aaattcatta attacatctt
     2701 ttgcagttgc atatacaaga tactttgttg aaactaagag aggtttttct ttgggatttt
     2761 ttagactgta tattttattt gctttatcat ttgcaataat aggttacttc tggatatata
     2821 aaggaataca tatttaaaaa agtattgaga ctattttatg ttaataataa gatagtcttt
     2881 tttctttttt aagaaaagat atgagtattt taagtagtgt gtaattttag acaaaagaat
     2941 gattagataa ataaagcaat taaagcaaga tataaatgca tgaaataaaa ttacaattta
     3001 aaatgtaaaa tatgaaaaaa tatatacaaa cgtattcctg taatcaaaaa tacatgctat
     3061 aatgattgaa aagaaataaa aaaacagggg gaatcaatat gagagtatta tttgtttgtt
     3121 catcagggat gtcaagtgct atagcagtta atgcattaca aaaagaagga gcaaaaaatg
     3181 gaatagatat agatgttttg gctgttggta ctcaagagtt tgagaatgaa gttaaaaatg
     3241 gatgggatat tgctatggta gctccacaag tcagacatag atttgactat ttaaaagctt
     3301 ttgctgatga ggttagtgta ccatgtgcat taatacaagc acaagcttat agtccattag
     3361 gaggaccaaa gttattaaaa caagttcaag aactgttgag taaatagtag ttgcagtgtt
     3421 gttagattag aagaaagttg attttataaa aattttaatg ataataaaaa taaatattaa
     3481 ttaaggagag aaaagatgct agataaattt acagaatttt tagacactaa attatcaact
     3541 tctatggcca gattggcaga acaacgtcac ttaagagctg taagagatgg aatcgtagct
     3601 tcattaccac ttataatagt tgggtcattt ttcttgatat tagcatttcc tccattacca
     3661 gattcatggg gggtaactca gtatctaaag gaaaatgcag ttacaatatt actaccatat
     3721 agaatgacat tttttattat atcagtatat tctacatttg gcataggtta tagtttggca
     3781 aagtcatata agttagatgg attgacagga ggtttattag ctacagctac attccttttg
     3841 acaatagttc ctactgcaat agagggtctt acccctgagt tattaaagat agttgaaaag
     3901 agtccagaac ttttagcttg gtataattca attcctcaag gatggcaaat gccaatggct
     3961 aatcttggtg gaggttcaat gtttgttgct attgtaacat ctataatagg tgttgagatt
     4021 tatagattta ctgacaaatc taaattcaag ataaccatgc cagaagcagt tccagcatca
     4081 gtcacaaggt cttttgaagc tctgacacca gccgcattaa taataatttt aatggctatt
     4141 gttacttatt tcctagggtt tgattggcat ggcttcatag ctaaattagt aactccatta
     4201 gtttcagcca ctgacacatt accatcagta attatactaa tatttttaat aacgtttttt
     4261 tggtcatttg gaatacatgg tgtatcaata ataggttcat tggcaagacc tttatggata
     4321 caattattag atgctaatac agcttcatat gctgcgggtc aagcagttcc aggtattgct
     4381 gctgaaccct tttttcaatg gtttatatgg attggaggtt ctggagctac aataggctta
     4441 gctttacttc ttgcatttag agcaaaatca gcttatggaa agtctttagg aaaagcaact
     4501 gtaataccag caatattcaa tataaatgaa ccaataatat ttggagcacc aatagtatta
     4561 aacccaatat taataatacc atttgttgta gcaccaatag taatggcaac agtttcatgg
     4621 tttgctatga gtgctaattt agtatccaga gtagtagtta ctgctccttg gacattacca
     4681 ggaccagtag gggcatattt agccactggt ggagattgga gagcttcagt tcttaactta
     4741 ttatgtatag ctatatcaat aataatttac tatccatttg tcaagatgta cgataagaga
     4801 ttgcttcatg aagaaaaagt tgaagagcaa gaagcttatt aattagaaac tatattggaa
     4861 attaaaaact atattagatt agtataaatt tcaaagtata acagttatga aataagtaac
     4921 gaagatattt tgttgattgt ttataaaaaa agaaaaggta aacttttctt tttttataaa
     4981 tatatatgaa actttttttc accaaatttt taaaaaagtt tcgtgtaatt cgaaataaat
     5041 ttaaacacat ataggatata aggagatgac acagatggga agattaggaa tatcaattta
     5101 tccagaaaaa acaacatcta aggaaatata tgattatata gataaagctg ctgaaaatgg
     5161 atttacacgt atattttcat gtttattatc agttaaagat actaaagaaa atataattaa
     5221 aaaatttaaa cgtataaatg aatacgctaa gtttaaaggt tttgaggtaa tacttgatgt
     5281 aaatcccaga gtatttggtg aattagaaat aagctacaaa gatttgactt tttttaaaaa
     5341 gacgaaggca gacggaataa ggcttgatat aggatttaca ggttctgaag aggcattaat
     5401 gacttttaat cctcaaaatc ttaaaataga aatcaatatg agtaataata ctaattatat
     5461 agatacaata atggactacc aaccaaataa agaaaacttg acaggatgtc ataactttta
     5521 tccacataga tatagtggat taaatttaga acactttata aaatgtacag aaagatttaa
     5581 taagtatgga ttaagaacag cagtttttgt aacaagtcaa aatgaaacta catttggacc
     5641 atggcctgta actgatgggc ttccaacaat tgagatgcac agaaatctac ctatagatat
     5701 tcaagtaaag catataatag ctatgaataa tataaatgat ataataatat caaattgtta
     5761 tccaacatat gaggagctaa aggtattagg ttctatgaga aaagatatgg ttacatttga
     5821 tgtagcaata gaggaaaata tacgagaagt tgagaagaag atactctttg aagaaataca
     5881 ttttaataga ggggacatat ctgacagtct gataaggtct actcaatctc gtgttaaata
     5941 taaaggatac aaatttgaac tatttaatac tcctaaaata ataaaaagag gagatatagt
     6001 aattgaaagt agtgaatatg gtcattatgc aggggagtta caaatagcct tgactgatat
     6061 gaaaaacagt ggtaaatcga atgtagtagg atatataaga aaagaagaat tatttatatt
     6121 agattatatt aaaccatggc aaaagtttaa ttttaaaccc tcaacaaaat aatttattat
     6181 gaaaatatta atttattata aaaatataat tatgaaagtt ggaattaaaa tgtgtttaaa
     6241 aatttaatat taagaaaatg aggaaatgat atgttaataa aaactttaga aaatttagtt
     6301 acagaaggta gaaataagaa tacattgcaa atagataaag aagatacatt aggcataata
     6361 gaattaataa ataacgaaga taaaactgta gcctatgctg ttgaagaaca aaaggagagt
     6421 atagcaaaag ctgttaatat aatagtagat agaatgaagc agggaggtag attattctat
     6481 ataggagcag gcaccagtgg taggatagga atactagatg ccacagaatg tcctccaact
     6541 tacggagtgg attttgagct tgttcaagct ataatagctg gtggaaatca agcaatattt
     6601 aaagcaatag aaggtgctga agatgataaa gaacttggaa agcaagatat aatagataga
     6661 ggagttacat ctaaggatgt tatatgtggt atagctgcat caggaaggac tccttatgtt
     6721 ataggtgcaa tggagtatgc taaggaattg gggtgtgcag tactatcaat aactatgaat
     6781 ccgaatagtg aaatgagtaa aaaagcggat ttaccaataa acattatagt aggggcagaa
     6841 gtaataatgg gttcaactag aatgaaaagt ggtacagctc aaaaaatggt atgtaatatg
     6901 ttaacaacag cttctatggt taagatggga aaagtatact caaacttgat ggtagatgtt
     6961 aagacatcaa atgaaaagtt ggttgaaaga gcaaaaagaa taataatgat agctacaaat
     7021 gtaaaatatg atgttgcaga aaaattctta gaagaagcag ataatagcgt taaattggct
     7081 atatttatga taaaaagtgg tttagataag gatagtgcaa agagtatcct agatagacaa
     7141 gaaggttata taagtgaagc tttaaaatca atagaaaaat tgtaaaataa ataatagtac
     7201 tgataacctt tcaatattaa aaaaacagca ttggaaggtt atttttagat attaagtatt
     7261 gaaagaatta ttaaagatat tgtaggagga tagtatgtat tttttaggtg tagatggtgg
     7321 aggaacgaaa acaacattta cattagtaga tgaagaatta aatatagtag gaaccataac
     7381 caaaggaact tgtcattata atcaaatagg atttgataat ctaacgaaat tattaataac
     7441 tgggttggaa gaagtttgta aagatgctaa gataaatgta gaagaaataa cgtatgcatt
     7501 tgttggactt gctggatatg gaaaaataaa agaagtattg tatgcattag aagtagcaac
     7561 aaaaaatgca tatagtcata taaattatac tctaggtaat gatgtagaaa tagcattagc
     7621 aggttcttta aatggggaaa aaggtataaa tataattgca ggtactggtt caatagcaca
     7681 ggctttagat aaagatggga atcttcacag atgtggagga tggggctatg tattaggaga
     7741 tgaaggttca gcgtattata taggaatggc taccttaaaa atgttcacaa tgcagtctga
     7801 tggaaggtgt tcaaaaacaa aattatatga tttaataaaa agacatctta atatagagaa
     7861 tgattatgac ataataaaat atgtaaatga tgaaatacaa ggagatagaa tagaaatagc
     7921 aaagtttgct acgatatgtt taaaagcagt tattgaaggt gataatactg ctagtaaaat
     7981 atttgatgat gcagcttatg aattaagtag gcttataata ggattagagc atcattttga
     8041 acaaggtaca aagataaagg tatcttattc aggtggagtg tttaagtcag gagatttaat
     8101 attagagcca cttaaaaaat atttgaataa aacgagattt ggtatagtga gaccagtgtt
     8161 gactccagat ttaggagcat gtttacttgc taagaagaaa tattatagta aataggtaga
     8221 ggtgatagag tatgagttgc attttaaaat taaaacagat atatgaagat ttaacggaag
     8281 tagataaaaa aatagctgat tacatactta ataatacaga ggctataagt aagctttctg
     8341 tatcagaatt ggcgtcaaac tcaaaaacaa gtacagcaag tatagttagg ttttctagaa
     8401 aaatgggata ttcgggtttt ggggatttaa aaattgagat agctaaagat ttgatgggca
     8461 aagaaaatga atacacatat gtagatgaaa actatgattg taatatagat agtgttataa
     8521 acaaaattac aaacaaaaac atagagacta tcaaccaaac aaggttgttg aatgaaaaag
     8581 atataataaa agaagcagtt gaacagataa taaaggctaa aaatgtatat atatttggag
     8641 taggtggttc ggctttagtt gcactagatt tacagatgaa acttttaagg ataaataaac
     8701 aggcatttac ctctttagat agtcatacac aacttatggt atcttcaaat gtagataaag
     8761 aagatatagc tatagcaata tcttactctg gagaatcaaa agaagtaata aagtctatag
     8821 aaaatgcaaa attaaaagga tgtaaggtca tttgtataac taaatatagt gacaatcatc
     8881 ttagtaagat tagtgattta aagctagtag taccaaatat agaaaaaaga cttagagaag
     8941 gagctatttc ttcaagaata gcaatgctta cgttgataga tataatatat atttctataa
     9001 ttcaagaaaa cttaaatgag gcagaacaga aactacaaga aacaaaaagg atacttgatt
     9061 atttaaatat taataatgct taaaactagg ttatatatca actaaacaaa taagttaggc
     9121 tataagcgta agtcaataag aaatagataa atatctaaaa tattgactta cttaataata
     9181 atatatagca tttttataat acaaatgagt tgtcttaaaa ttgatttttt ttaatcaaat
     9241 tttaagttca actccttttt tatattttta tagatatatc ctatattttc ccagaaaacg
     9301 tattgaatat acaaattata agaagtataa tgttgttggg agagaatgta tgaaatgtat
     9361 acaaagttga ttttaactgt attattgata attctcttta ctcttaccat tattaatgat
     9421 tgaagacaat ggtatttatt ataggtcaaa gattccgaat gtattaatca tcatcatttt
     9481 ctcaaactaa cagcaaatac cctgatttgc gagttgtatt gtatacaaat tatctagaaa
     9541 atatctatta gattttccat aaaatttaaa tatttcacga atgtatgaat ggagaaatat
     9601 acatgttgac aatccacctt tttaaatact aaattaattt tattaatggt agagaatagg
     9661 aagtaacaat gttttataaa ggagaatatt aagtatgaag aaaattagga agttaactac
     9721 caagttattg attacaacaa tagttttaat ctcaggtatg agtatgacag tttatggtat
     9781 gacagccaaa gaggtaacag caaaaacacc aaaaagctat gtcactggaa ccaatagcgt
     9841 atatggtcca aagctgtcac aagcccaact aaactcagta gcacaagcaa cagcagattt
     9901 tatgaacaag aaaatcacaa agaatatgac cacagatgct aagattttag ttgcctacaa
     9961 tcacattaaa aataacacaa catatgttga ctggaatgca gtagagggag caaatactgc
    10021 ctatacattg gttactaaaa aaggtgcatg ctcaggaatg gctaggagca tgaaagcttt
    10081 gtgtgatgct atgggaattg aatcttatta tgtacacagc acatcaaatg accaccagtg
    10141 gaatcttatc cgttttggtg atgggcgttt gtatcatgta gatattgatg ctaataaatc
    10201 agcaggaaag gatatcatat acaagagtct tagccaccca ttaccatttg ataagacagc
    10261 atatccaaaa gttggtgcaa tagtacagac ggtaaaacca gaacagacac agaaaccagc
    10321 accacctgga aagcacacta tgttaccaca tcaagaaggg gcaaaggctg ttggaaattt
    10381 tggaaaaaaa ggagaagata taaatgaaaa acatgtaatg tatgaaggtg ttagatgtag
    10441 tgtagttaat tcatcagtaa atacaattac atttacaggt agtgaatcaa atttagaccc
    10501 atcaaatctt acgattgctg taataggatt tatgaacaca ccatatggga tgtttgcatt
    10561 agcaccagag ggtaaacctg ttgaatatcc ttttaaatat gataagccat tttctttaga
    10621 ttttagtggt aaaaatcgtt ctggtgcaga attagcgaag tactgtaaaa aaaatgatgt
    10681 gactattaaa atagaaatca ctgatgaaaa agctaatatt ggacttggtc aaataatgta
    10741 tgttcgatat gaataatgat ttaatcatga cagtagaata agttataaac attgcgccct
    10801 cttactaggg gcgcatttaa tattatatct gatattttgt agtcaggtca actgctagat
    10861 ttctcccctt tttatttata aaaattatta tctctaagca tctgagcatg tttacgatta
    10921 actaaaaact gttttaactc agtcaagcat aggaataaaa tagctctatt ttcaaactct
    10981 gttctgtcaa caggcagagg ctggtttttc atactaagca ttagtctctt taattcttca
    11041 agaagaagat tgcctgtttc agcttcaaat tcagtataac ctatcttatg tataaaagca
    11101 gatagaatat gagcttgtgg tggtgtagaa tttagttgag atacataact atacaaattt
    11161 tctaagatat cccgttggct ttttataata tccatatgtt ctaagaagaa tctagtatca
    11221 cttaataaag tattattaat attatcgtaa gtttcagaaa tactgctgtc aataagtgaa
    11281 tttaatgtat ataaatctct actatatcca ttttcttttt taggatttac aataatatct
    11341 gcaatgtcta taagtacaat actcacttct tcctgtaact ttttttgaac ttcatggatt
    11401 ttatgtatat ttgttggaat atacatattt attaagacac ctattccagt tcctactgca
    11461 aatattccag cttcatttaa aatccatttg atagaagttt ctccttgaag gtagtaatgg
    11521 gatgctatga ctacacacat agcaataaca tcttgaatat ttaacaaaaa acaaagtggt
    11581 ataattataa atataaatat tgaaaatgaa gtcaaattat atcctaaaat attaaaacat
    11641 aaatatgaaa aaatagtaca taaaagaaaa ccataaatct ttccaatgga gcctttaaga
    11701 gtttctcttt tagtgtcctt tactgtaagt agtactataa ctccagcaac cattgaatat
    11761 tctaatttta atagccaagc aataaatata gcaagagaac atccaagaga taattttatt
    11821 actttaagtg aattaatatt attcaaatgc aaacctcctg taaatcaata taataaaatt
    11881 ctaacataaa actaatattg cttgaaaggt aagtaactat acttaattat tttattgccc
    11941 tatttgttta gatacaatca atataatttt attttatgaa aattttttgt aaaattttaa
    12001 atttagagac taacatgaga ccacaaagag gaagtttatt ataagaagaa aacttaaaat
    12061 tgattttaaa tagcaaatga aaataatcaa atatgtatat actattatta aaaaataaaa
    12121 ttatatagtt aatataattt tataattggt ttagtataga gatttttaac ttttatgtta
    12181 tactatttat aaaagtaggt ataaagatta aaatacataa tcaaatatgt ataagaggtt
    12241 ttaaatagta ttgttaatga acttgattat tgtttataat tacaaattgg aggagataaa
    12301 catgaaaatt actgaaggat atatgccttt taaaggtttt aaaacgtatt accgtatagt
    12361 aggagaaaat acggaaggga agaagccatt agtattactt catggaggtc caggttctac
    12421 acacaactat tttgaggtat tggataagat agctgaaagt ggtagacaag taataatgta
    12481 tgaccaaatt ggttgtggaa attcatttgt agaagggcat cctgagctat ttaatgcaga
    12541 tacttggata gaggagctta ttgaactaag aaaacatcta ggacttgatg aaattcatct
    12601 gttaggtcaa tcttggggag gtatgcaggc tatttggtat gctattgaat ataaaccaaa
    12661 aggtattaag tcttacatcc tttcatctac tctttcttct gcaaaacttt gggagaaaga
    12721 acaaaaaaga agaatatcat atatgagtga ggttgaccaa aaagcattac ttgatgcagt
    12781 aaatactgga gattactcta gcaaagaata taatgatgca ttagaaagat ttatggaaat
    12841 gtattgtgca ggtgaagtga cagaagattc tccagagtgc ctgagaagac ctaaaaaatc
    12901 aggttctgaa gcatatattg taggatgggg acaaaatgaa ttttctccta caggaactct
    12961 ttctggatat gaatttacag atagattaca tgaaataaaa gagccttgtc tagtaactag
    13021 tggagcaata gatttatgtt caccatatat tgcaaagact atgtatgata gaatacctaa
    13081 tagtaagtgg gaattatttg agtattcaag acatatgcca ttcgttgaag aaaatgagaa
    13141 atatataaaa gtcttaactg agtggttgaa tgcgaacgat taaaaactat ataaacttat
    13201 ataaaaaaat gagtgtctca aaatgaactt tttagttcat taggcactct ttttgcatga
    13261 aatcaaatat attttcatta tttgaacgat aaaatgaggc ttatatctac tttgggaaag
    13321 taatttttta ttactagcgt tagattctaa tctaattgta tacgtattga aataatagaa
    13381 atatattggt gtttataact taattttatt gttttttaga aattttttta tatattggta
    13441 taattattct gtttaaattt gattaaaaag tatattaatt ataataagaa acaattatat
    13501 aaataggaat agattattat aattaatata gatataaaaa taattataga aagaaaggca
    13561 cattatggaa aaattgagag ttattgaaat gataaaaaat aatcccaata ttatagctac
    13621 aatagataat ccaactgatg aaatgaaatt gttagctatt aaagaaaatg ggcttgtact
    13681 agaatatata aataatccaa ccagagagat gcaagaacta gcaatagata ataatattag
    13741 agcaattaaa tttatagata atccaacaga agatatgatg ataaaagcag taaatgaggg
    13801 atggagtata ctggattata tcaaaaaccc aactgataaa gttattgaaa tggctataaa
    13861 tcaagcagga tgggcaatta aatatgctaa aaatccaagt gaagaattac aactgttagc
    13921 agtaagaaaa aattatgatt cgataaagtt tataaaacaa ccttatgaaa gtgtacaaga
    13981 ggaggcagtg agaataagtt atgatgcctt gagatatata gattcaccta gttataatgc
    14041 agaacttata gctataaaaa ataatgaagc agctattagc tttataactg atttagataa
    14101 aaataaaatg ttggaatttt taaaagtgaa tattttagtg attaagtaca ttctgaaaaa
    14161 agtatctaaa gatgaattag agagtgtatt aaaagaagta ttatccaaag aagatgttga
    14221 ggaaaagtat attagagatt ttttgaattg tagtgttata gatagaaata gcgaaagtat
    14281 accaatagat aaaattatgt ttatttataa atatggaagt aagacagcaa aaaaaatagc
    14341 tgtagatgag aaattaaaga ttatgtagga gagagaaaaa tgaattttat agacacatta
    14401 aaaagcgttg acttaatatt atccactgga gaagtacctt taatagtagg tgaaagtgga
    14461 ataggaaaaa cagctttagc aaataagctt gccaaagaaa atgattggag tttaattgtt
    14521 attgatggaa acttacttaa agagggtgaa ataggtggtc ttccaactat agaatcttat
    14581 gtaggagtta attctaatgg atataagact gaaaagaaaa caacagtgta tgctgttcat
    14641 aacaaattaa gagaaattga tgaagagata tctaaaagta agacagttct tttatttata
    14701 gatgagataa atcgttgtga gcatacagta cagcaagaac ttatgaattt aattttaaat
    14761 agagaaatta atggatataa actgcatgat gatgtaaaga ttttagcagc aatgaatcca
    14821 tcaagtaaat atggttcaga ttttgattat caagtggtag atatggatgc agcccaagaa
    14881 aataggtttg tttggttaaa tatggagagt gaccatacac aatggataaa atgggcaata
    14941 gatgagggta ttgaaagaaa agttatcgag tttatttcca cttttccaga atatctgcat
    15001 aaaataaatg aagatgatgt aagagccact cctagaagtt atgagagagt atctaaaatt
    15061 tataaagttt ataaagaaaa aaataattct attcctagag cagtattttt aaatgttgta
    15121 aagggtaatg taggtaaagt tattgcagaa gaatttataa gttttattga atcaaactct
    15181 gaaccattaa tatcctatga agatgttttt ttaggagaat ctattgatga gtctattgta
    15241 gaaagagtaa aaaatgagag tcatacaaga ctttatctat cagctatgaa cattctaaaa
    15301 gacttagaat taaatattaa gaatgataaa tatgaatcaa accactatat taatagattt
    15361 attgaatttt taaaaatgta tcctgtagat ttaatgatag gtattatgaa ggatattaga
    15421 aacagttata ttgaagtata taaaaaagct atagaaaatg aagagtttgt aaaatcatat
    15481 tttgaatctt atagtttaat aaggggataa tgtatggaaa attattttga taaacaagca
    15541 aaagagcttt ataataaagc aagtgaaatt ataaatactc attctatgct aaaggcaaat
    15601 cgtgctaatg agaaatttga tatagatata ccacaagatt ttaagtatga gttttttagt
    15661 cttgtagata aagtgaattt aagtcttatg gaagaagaag ataattttta tggatatttt
    15721 ttgtttcaga tgtcaagaga aataagattt gatataagta gtcctacagg tgtgaatttt
    15781 aagggagcta aatatgttat atattttaat ccaataatct ttttaacgct tgacataaag
    15841 caaatggaga ctacaattaa acatgaaatt catcatatat tatctatgca tttgatgaga
    15901 gcaaaggagt taaaaggaaa gtatagtaca ttagcaatta atatggcaat ggatatagta
    15961 gtaaataaat ttctaaacaa tttaccacca tatgcaacta ccttagagtg ggtaaattta
    16021 aagtattcct taaaacttga accatatgaa ccttttgaat attatgtaga aaagattcag
    16081 actgaattag atttgatgga agtggatgaa gatggtgagg aagatgatag tgataaatat
    16141 ggagatatag aaattgatta tagccccgaa agaactcatg atatatggga tgaatccaat
    16201 gagatagatg aaaataccct tagagaattt actgaaaaat ttattaacag ttctcaaaaa
    16261 ggtaaagttc ctgcttattt agaaagtatg atatcatcac ttaaaaatag taagggtgaa
    16321 ttgccatgga atttatatct taatagaata atgggaacag ttgaaaataa taaaaagaag
    16381 actataacaa gaagaaatag aagacaacca aatcgtttag atttgagggg agaacttaga
    16441 agtcataagt cagaaatagc agttgcactt gatgtaagtg gaagtattag tgatgaagag
    16501 tttaaacaag ctattaaaga agttcttagt atagtgaaaa actataatca tgaaattaca
    16561 attatagaat gtgataatga aattagacgt gtatataaag tgagaactga aaaagatata
    16621 aaagatagaa ttaggatagg tggaggtact agattttctc cagtctttga gtatgctaat
    16681 aataaaaaga ttaatttgtt agtctacttt actgatggta aaggtgaaaa taaactacaa
    16741 gtaataccta gagggtataa aattttatgg gttatttctg gaagaggaga taaaatttca
    16801 ttaaaagaac cttatggagc agttaaaaaa cttactaagg ttaaaacaaa tgatgaaata
    16861 ttagaaataa atgatgtaag aaatgatggg tactcaatga atagccaagc accaatttta
    16921 taggtattca caggagataa gctgtattaa tttattttga tatgcttgtt ataaaaatct
    16981 aaaaaataca ttaatcaatg gttaatgtat tttttaattt taataatata agttttactt
    17041 acatttaagt cttaaaatgt gttaaaatat aatatatagc agatagtttt ggcaaggtgg
    17101 atttagttgt caaaactatt ttttttatta aggatggtga tgtttttgtt taagattgat
    17161 gattatataa tgtatggtat gacaggagtc tgcaaagtca tggatataac aaatgaacga
    17221 tttacaaatg gaataaaaaa agagtattat gtattgagtc ctatttattc taataataca
    17281 gtaataaaaa ttcctgtaga taatgaaaaa gtaccaatga gaaagttact ttctaaaatt
    17341 aatgtgcttt cattaataaa tgatattcct aatatggata catcatggat agataatgaa
    17401 aaattgagaa gtgagcaatt taaaaaaata ctcagaggtg gtaaatgtga agagttaata
    17461 aaattagtaa gaagtataga taataacaga gagtatgtaa aatctattgg taaaaaaaca
    17521 catcaagcag atgataatat aatgaaagaa gctgaaaggt tattaaatga agaatttgca
    17581 actattttag atatttctcc taatgaagtt agttcatata tttcaagtca tatacctcaa
    17641 taaagtgttt tagtatcctt tatttaatct attttaagta ttatttatgt atgctggttt
    17701 tggtaaaata aaatatataa ttaaattata taaaaaataa taatgattgt attgaagaat
    17761 tacagaatag tgcggagagc ctactccttt aggtgtgaga tggatatcac ttaaagattt
    17821 aaattgttat tgtagatgtt tttaatataa aacaagatta ataagtgtat tgtaagcttt
    17881 aagtgaaatt gatactataa tgagttactt agattttaaa atatataata ttataaaaaa
    17941 tccttttagg ttttgaatga ataagatgga tactatgttg aatagaatcc atcttattta
    18001 agttttattt atgttgttta tttgtaagca ttatttttgt acatgaaaac acagtgtaaa
    18061 aatatgctta gaattatgaa gttttattaa aaagatattc gacaaaataa gactaaaaat
    18121 attcttaaac ttataactaa gataaagatt gtatatttat agtgtagcct gaaaaattgt
    18181 ggatgtacga aatttattta gtattttatt gaaacaaagt agaagaagta ctatagttta
    18241 tctttgaata gattgttatt ttttagtgaa aaagttatta ggttaataca taaatataat
    18301 agcttataaa tagtttaagc atgt
//
LOCUS       DAEMGG010000077        18312 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEMGG010000077 DAEMGG010000000
VERSION     DAEMGG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.077"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(44..1000)
                     /locus_tag="KPK24_002430"
     CDS             complement(44..1000)
                     /locus_tag="KPK24_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG2300098.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GHGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            1368..2156
                     /locus_tag="KPK24_002431"
     CDS             1368..2156
                     /locus_tag="KPK24_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2300099.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(2279..2749)
                     /locus_tag="KPK24_002432"
     CDS             complement(2279..2749)
                     /locus_tag="KPK24_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG2300100.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            2888..3532
                     /gene="uppS"
                     /locus_tag="KPK24_002433"
     CDS             2888..3532
                     /gene="uppS"
                     /locus_tag="KPK24_002433"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG2300101.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            3575..4480
                     /locus_tag="KPK24_002434"
     CDS             3575..4480
                     /locus_tag="KPK24_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2300102.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            4795..6342
                     /locus_tag="KPK24_002435"
     CDS             4795..6342
                     /locus_tag="KPK24_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2300103.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            6345..6743
                     /locus_tag="KPK24_002436"
     CDS             6345..6743
                     /locus_tag="KPK24_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG2300104.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            6783..8516
                     /locus_tag="KPK24_002437"
     CDS             6783..8516
                     /locus_tag="KPK24_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG2300105.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            8748..9959
                     /locus_tag="KPK24_002438"
     CDS             8748..9959
                     /locus_tag="KPK24_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG2300106.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            10152..10412
                     /locus_tag="KPK24_002439"
     CDS             10152..10412
                     /locus_tag="KPK24_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG2300107.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            10766..12478
                     /gene="ptsP"
                     /locus_tag="KPK24_002440"
     CDS             10766..12478
                     /gene="ptsP"
                     /locus_tag="KPK24_002440"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG2300108.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            12801..14351
                     /locus_tag="KPK24_002441"
     CDS             12801..14351
                     /locus_tag="KPK24_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2300109.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            14377..17079
                     /locus_tag="KPK24_002442"
     CDS             14377..17079
                     /locus_tag="KPK24_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2300110.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSAQQIVHKQCANDILSCIENCGLDKKYISVEVTEKVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQITKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(17585..18250)
                     /locus_tag="KPK24_002443"
     CDS             complement(17585..18250)
                     /locus_tag="KPK24_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300111.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT         7278 a         1779 c         3033 g         6222 t
ORIGIN      
        1 aggtatgtat ttatcatcaa attattggtt tgttttggaa tttctaatct tcaaccataa
       61 tatctttaaa tatgaaatca tgcattgcat ctagttcata ttcctcaaaa ggtattacat
      121 agcctgcatt acctattttt ctaccatttt cagtagaaag aggaaactcc atagatttca
      181 aatttaaatt tcctatactt aaaagttctt ttcctaaaga tattatttca ttaggattca
      241 tattagtttt tacatacgga agtattgtgt taactagatt aggatattta gtgactggta
      301 aatccttaac accatttatt aaactctgta taacacttct ttgtcttcta tctctttcat
      361 ctgtactatc attttttctt atacgagaaa atgcaagtgt ctgatatcca tttaattttt
      421 gctcaccaga atttcttata tatttaagag aacctttatt gtcattttta ttccatttat
      481 aagtttcagg aataaattta tttaattctc ttatttcact cttatcaaca gtaacagtaa
      541 ctccacctaa agcatcaact atatccataa aagagaaaaa gtcaactacg gcgtagtttt
      601 gtatatcaag ttggaagttt tcttctatag tttctattaa taagttagct tgtccatata
      661 cgtaggcatg tgttaatttt tgttctccat gtcctggtat attaacataa gtatctctat
      721 ttaatgaagt tagcttaaga cttttatttt tattatcaat agtaagtatc atcatggcat
      781 cagaacgaga aacttcttct cctggtcttg catctgtacc taacaataat atgtttgtga
      841 tattttcttc gtttttatag tcattactac taagcgcctc tgtatttata ctatcatctt
      901 gtatagtttt taatttagta aaaacatatc catatatact aacaggaaat gctataacta
      961 aaagggctag aaatataacg aactttttga gtcctgacaa taatatcccc tactttctaa
     1021 attttttaat tacacatagt taattatatt aaaaatttgt tacataagca ataaaaaaca
     1081 caataaatta gcataagttt gtcataatat aattattttt atagcatttt atagaagaaa
     1141 gtgtgtagtt attattgatt ttattggaat ataattgtat gaagacttaa atttttataa
     1201 actaatgtgt atattgaaaa aaatattttc ctgaatgtag ttatagcatc ttatgaaagg
     1261 tgcaaattgt aatttaaaat ttttagtgta taataagaat tgaaaattca aaccgataaa
     1321 aattaataaa aaataattat aaattaataa tttgggggag gaaatgtatg ataaaattaa
     1381 tagcaacaga tttagatgga actttgttag atgaaaagag cgaaataaat ccagagtttt
     1441 ataaagtatt taaaaaatta agagagagag ggataatgtt ttctgctgct agtggcaggc
     1501 agtatcaaaa tctgataaaa aaatttgaag atataaaaga tgatatgatg tttatatcag
     1561 aaaatggaac actagtagta tataaaggaa aagaaatatt atcaaatcct ttaaataaag
     1621 aattagtaaa tgaaatcata gagacaacaa gaagtataaa agggaaaaaa attgtaatga
     1681 gtgggaaaaa atacgcttat atagaaagca aagatgaagc gtttatacaa gaggttagta
     1741 cttattatgc aaagtttaaa attgtagaag atttgacgaa agtagaggga gacatattga
     1801 agatagctgt gtttgacttc aaaggtgcag aacataataa taatatctac tttgaaaagt
     1861 tttctgatag agctcaagta tgtatttctg gagtggagtg gcttgattta actgcaaaag
     1921 gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat gttagatata aaatatgaag
     1981 aaactatggt atttggtgac cagttaaatg atgtggaaat gatgaaaagt gcctatcata
     2041 gttatgccat ggaaaatgca aatgaacatt tgaaacaaat tgctagattt agagcaaaaa
     2101 gaaatactga aaatggcgtt gtagataaga taaaagaggt tataaaaatt ggatagtcat
     2161 atgtatagat gaaaaagatt attttgaaga aatcaaaaaa atatcaatgt aattttgata
     2221 aataagttta tttatgcgaa aattacattg atatttaata aacatcaata atagatattt
     2281 atttttttat gatattcagt attttgtttt ctatttgagt tgcattttca gcagaaatta
     2341 aataaacata cctacctgcc ttctcaatta tagtattatt tgctatttca gaattgattt
     2401 tttctccatg tccttctcca aaaggtgctt gtatcatatc ttttttataa ttttctagag
     2461 ttttatagat tttatctacc tcgtcagttt ttataacgat aatatcttct agagataatt
     2521 tttcagaaga attaattaca aaaccttgaa ttatgtcttc ctctacatca ttaaaatagt
     2581 taaaatcctt tgcatcaaag atttttgatt cctttataag taaatcagaa gattttaatg
     2641 ctttttttat ttttgatgta gaaacgtttt tgtaatcacc acttgaagaa caactaacca
     2701 ttcctaatga taagattatc gaaaaaaaca atattaaaat ttttttcatg ataaacctcc
     2761 aagtttttta aactctgtct taaattagtt tactacataa ttatataaaa ataaatgtat
     2821 ttaatgttac agttttgatt aataatgtaa tattttgata ataataccta ttgagaggtg
     2881 attagaaatg agaataccaa aacatgtagg aattataccg gatggaaata gaaggtgggc
     2941 aagtcaaaat ggtatgacaa aagataaagg atatgaaagt ggaatagacc ctggactaga
     3001 gtcttataga ttatgtagag atttaggctg tgaagaggtg actttttatg gatttacaac
     3061 tgataataca agacgtccat cagtgcagac aaaagctttt acaaaagcat gtattgatgc
     3121 tgcaaatata ttaatagatg aaggtgctgc tttattagtt gtaggaaata caaaatcgcc
     3181 aatgtttcca gaagaattat taccatatac taatagaatt aaagccaaag aaggtcaaat
     3241 aaaagcaaat cttctggtaa attatggttg gtattgggac ttaaataata tatcaggagt
     3301 agaaaatgta aataaaaata atatacagga ttacatacag tcatttgatg tatccagact
     3361 agacttgata atcagatggg gtggaagaag gagattaagt ggtttccttc ctgtacaatc
     3421 aatttatgca gatttttatg ttatagataa ttattggcca gattttaaga gtgacgatat
     3481 aaaggacgca tttagttggt atgatacaca agatgtcact ttaggtggat aattaaaaat
     3541 attctgattt aaggttgtag agtgttttaa attattgaaa aaaaaacttc ttgatggtaa
     3601 aataacacta ataatatgta aaagtgtaaa aatttatacc aagaagggtg aagaaatgtc
     3661 aaaaggcaat aataacaata attcaagaaa caaatctaaa aagaccagtc atctgaatag
     3721 aaaaaagcga aaattaaata agaaaaagtt agcagtgttg atttgcttta cagttttatt
     3781 tttatttatt gcatttaagg ctactcaagg tgtagtagct ttggtaaaaa gtatggataa
     3841 aagtaataaa actagtcaac aacaaaatgt taactcagaa caatttgacc ttggtaatga
     3901 agaggaaaat aaaaagaaaa aatatactgt atttatagac ccaggtcatg gaggcaatga
     3961 taaaggaact gaaagcaaga ccagtaatag atatgaaaaa gacctaaatc tacaaattgc
     4021 aaaaaaatta gcaaataaat tatctaaaca aaaggatatt caagtagtag tatccagaac
     4081 tgatgataca tatatatcat taaaggatag agctatctta gcaaataata gttctgctga
     4141 tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt aatacagcaa caggtataga
     4201 aacttggtat aggaataaag ctacagatgg ttcaaaagaa cttgcacaga cagtacaaag
     4261 tactatagta tcctatgtta aagttagaga taggggaata gttgaaaata actttgaagt
     4321 acttagagaa tcaaatatgc ctgctatatt aatagagtgt ggttttttaa caacaccttc
     4381 agaggaacaa aaaataataa atgaaaaata tcaagaccaa cttgcggaag gaattgttca
     4441 aggtgtactt tcatacctag attctaaagg gaataagtaa attaacttta ctttaatgtg
     4501 aggataaatt taaaagttta actgtgtaaa ttgtattaac tttatttcgt aatgttggaa
     4561 atattgtttg aatttagaaa ctatgaaatt agaaattatt ttatctatat ctaatttcat
     4621 agtttttttt gcaaaaaatt tatagttata aaattttaaa aaataaattt agtataatgt
     4681 aatgtagtta tttatatgtg attttaactt gcattgattt gctatcaatt gtgctttata
     4741 attagtatat ataaagttta actgtaagat aatttaaaaa ataggggaat ttatatgaat
     4801 aaaaatagca tgacaagaat ttacagttta aataaaataa tttttattat aattggaatt
     4861 ttaggaatag ttttactcac aagacatatt gaggtagata ttaaagtttt aattttaatt
     4921 ttattaacct tgtttagtat aaatgtattt atttcatatc ttaaaattaa gttgtatgag
     4981 gataaaatta atttaggatt gataaataat tcagataagt ttataaatat tactaaagat
     5041 gagaacttcc taaaacatgt tcaaaattat attatcagta atgaaaaaga aaactgtgta
     5101 atggcttgtt ttgatttatg tagactcaaa cttataaatg atatatatgg ttatgagcta
     5161 ggggatgaag tgttaaataa tattttaagt aacttaaaaa attattttgg aaaagaagct
     5221 atttatggaa agttaaaaag tgatgttttt gtattaataa taaagcttga aaatagggaa
     5281 cagaagattc cttatttagt aaatcttatt aagaataaaa ttgtgatact aagtcagatt
     5341 gacagtttta aaatggatat aaatattgag gcttctattg gaatttataa atttaacaat
     5401 aatgaaattg atataaaaaa agctatagat aatgctgata tggcaaggct taaatcaaaa
     5461 ggactaaagc acatagagta tgtagaattt gataatgcta tggaagaaga aatacaatca
     5521 attatgaaaa tagagagaga tttattcttg gctattaaaa ataaagagtt tgtaattcac
     5581 tatcaaccta aagttgattc aagtacaggt aacattatag gttcagaggc attgataaga
     5641 tggttgcacc catcattagg catggttgga ccaaataagt ttatacctat agctgaaaaa
     5701 aatggtctga taaacaatat tggtagatgg ctaattcaag aagtttttat taccattaac
     5761 aagtggattt cagaaggtat aaatgtctta ccagtctcaa taaatctttc tagagtagaa
     5821 ctatataaaa atgatttagt tgattttttt aaactgatgt ttaatacata taatattcca
     5881 aaagaactta tagaattaga aattacagaa acgacagcac taagagatgt aaaatttata
     5941 agtgaaaggc tttatgagat taaatcttta ggtatgaata tatcattaga tgattttgga
     6001 gtaggtaatt caaattttat caaccttaag gggataccac tagatattat aaaaattgat
     6061 aggtcactta tattggatat agttacaaat acaaaaacaa agtttatggt gaaagcaatt
     6121 gttaaccttt cacatgattt aaacgtaact gttatatgtg aaggggtaga ggatatgcat
     6181 caggtcaaag tcttaagtga actgggatgt aatttcatac aaggttatgt attttttaaa
     6241 cctttggatg agatgaatta taaaaaactt cttactgacg gaagttatat aaatctgaaa
     6301 gatactcttt tagataaatg catgacagtt gaggaggagt aaggatgatt tcagaagatg
     6361 tagattattc aataagaatc ataagagcta tatttaataa tggagaagat aaaccaatag
     6421 atgctaaaat gatattaaaa agtgaagata taccaatact aacactatac agactgctta
     6481 gaaagctttg tgcaaacaat attcttactt ttaacaaaga agatggcaag acatacatgt
     6541 taaatgtgtc attggataca ataagtctaa gaactattgt agaagtaatt gatggaaaaa
     6601 tatatataaa taaatgtacg agatatgtat ctaattgcag taattgctta aaaggttgta
     6661 aagtaaatcg tgaattgcta gaaatagaaa aagaaattta taaactttta tcgagaaaat
     6721 ctataagaca aatactagaa taaaaaattt tcagctatga taataattgg aggttttcgg
     6781 ttatggatga tatatctcaa gataattttc tactgtctaa agagtatgag aatagcttag
     6841 atgtagatac taaaaaagca agtggaatat attatactcc taaaataatt gtagattata
     6901 ttgtgaaaaa aactttaaaa aatcacgata taattaaaaa tccatatcca cgaatacttg
     6961 atatttcctg tgggtgtgga aattttcttt tagaagtata tgatatttta tatgacttat
     7021 ttgaggaaaa tatatatgaa ttgaaaaaga agtatgatga aaattattgg actgttgata
     7081 acatacaccg tcatatatta aactattgta tatatggtgc tgatattgat gaaaaagcta
     7141 taagtatatt aaaagatagc ttaacaaata aaaaggtagt aaatgattta gatgaatctg
     7201 atataaagat taatctattt tgttgtgata gtcttaaaaa gaaatggcga tataagtttg
     7261 attacatagt gggtaatcca ccctatattg gtcataaaaa acttgaaaag aaatataaaa
     7321 aatttctcct agaaaaatat agtgaagttt ataaggataa agctgactta tatttttgtt
     7381 tttataaaaa gattatagat attttaaagc aaggagggat tggcagtgtt ataactccaa
     7441 gatatttttt agaaagtcta tcaggtaaag atttaagaga atatattaaa tcaaatgtca
     7501 atgttcaaga aatagtggat tttttaggtg caaatatatt taaaaatata ggggtatcta
     7561 gttgtattct aacttttgat aagaaaaaaa caaaagagac atatatagat gtatttaaaa
     7621 tcaaaaatga ggatatatgt ataaataaat ttgaaacatt ggaagaactt ctaaagagta
     7681 gcaaatttga acattttaat ataaatcaga gattgctatc tgacgaatgg atacttgtaa
     7741 ataaagatga tgaaactttt tataataaga tacaagaaaa atgtaaatat agcttggaag
     7801 atatagcaat aagttttcaa ggaataatta cagggtgtga taaagcgttt attttaagta
     7861 aagatgatgt aaagttgaac cttgtagatg ataaattttt aaaatgttgg ataaaaagta
     7921 agaatataaa taaatatata gtagataaaa gtgaatatag gctaatatac tcaaatgata
     7981 tagataatga gaatacaaat aaaaggatac tagatgaaat tatagggcta tataaaacaa
     8041 aattagaaaa cagaagagaa tgtaaaagtg gaataaggaa atggtatgaa cttcaatggg
     8101 gtagagaaaa gcttttcttt gaaagaaaaa agataatgta tccttataaa tcaaatgaaa
     8161 atagatttgc tatagattat gataataatt tttcaagtgc agacgtatat tcttttttta
     8221 taaaagaaga gtatttagat aaattttcat atgaatatct agttggaatt ttaaattcca
     8281 gtgtttatga taaatacttt aaaataactg ctaaaaaaat gagtaaaaat atatatgatt
     8341 actatcctaa taaggttatg aagataagga tttttaggga taataattat gaagagatag
     8401 agaatttatc aaagcaaata atatcaattt tattaaataa gtctatagat aaaggaaaag
     8461 tagaaaaatt gcagataaaa atggataact tgataatgga ttctttaggt atataagcag
     8521 ttgtaagtat taaaaaatta taatctttta attgtttatt taaatttaga aataaaaagt
     8581 tagccatata aaaaatatta aaaatatata cttttcaata tttttttgat aaaataagaa
     8641 tgaatttgtg caattttatt tttctccaat aactaaaaat agttttttga attaatattt
     8701 gcttaagaat aaaaatcaga aacgtttttg atatagtagg tgataatatg aaaggattag
     8761 ttttagaagg tggaggaaca aaaggagcct accaaatagg tgcatataaa gctttaagag
     8821 agcttggtat agagtttcaa ggagtaactg gcacatcaat tggtgcttta aatggagcat
     8881 atataattca aaatgatatt gaagtaatgg aagatatttg gttaaattat gactatactc
     8941 attttatgaa tattgataga gaaacctatg agaggtttaa aaatgtagat ttcactacta
     9001 aaaatctcaa tgcagtgttt gagcttataa ataaagctag aaaaaatgaa ggtatagata
     9061 taagcccatt aagacaatta atggagacaa cattaaatga agacataata aggaaatcaa
     9121 aaaaagactt tggacttgtt acggtctggt gggataaaaa aattaatcca tatccattgt
     9181 atatcgaaga tatacctaaa ggaagattga ttgattattt aattgctagt tccagtttgc
     9241 ctatttttca aacgaacatg atggatgata agttgtatct tgatggaatg ttttgtgata
     9301 acatgccaat aggactcttg gaagacaaag gttatgaaga tgttgttgta attaggttga
     9361 ttgatgattt tattgggaaa atgaatctaa atcgacatca aaatataaat ataaaaacta
     9421 taataccatc agaatatcta ggaggttctc ttaataaaga tagagataat gtagaaaaaa
     9481 acatccgact aggttactta gatactatga aagcatatga aagatgcact ggaattaagt
     9541 attattttga tgtagagtat aaatataatg aagaatattg ttttgataaa tttagaagta
     9601 tgagcaatga aacaatacaa tatatctgta atttattgaa tataaaaaga gatgcttcac
     9661 ttagaactgt tatggaaagt gtaataccaa agcttggtga ggtgcttgga ttgccaaagg
     9721 atttttcata taaagatttg ttttactgta tatatgaaaa gaaacttgaa gaaaataata
     9781 taaacaggat aaaattatat gattttaaca aggttataga tgttgtaaat acaaatattg
     9841 attctaatag aaatttagct tctaattttg aagttagacc taaaattagt attaaaaaga
     9901 acaaaaacac aaaattattg acaaattgta ttataaaaga cataaaaaat caaagttaaa
     9961 taaaaaacaa aaaaaaattt gtaattatac tcgaaaaaag tgtatattag tagtataata
    10021 tttttgtagc actagtggga acaaaaaatg accataggac aaaaaagtac cacttagtac
    10081 cattaaagta aaaaacagga aaacaatttt aaaaaatata aaatgtaaag tttataggag
    10141 gatttaaaaa aatggaaaaa gtagttagta tcaaaaatgc aagtggatta catgctagac
    10201 cagcaggaat gtttgttaag aaggcagctg aatttaaatc aacagtagag gttgtagcaa
    10261 aagacaaaac tgtaaatgca aaatcaataa tgggaataat gagtttaggt ttagctcaag
    10321 gagaagaatt aaagttagtt gtaaatggag aagatgaaga agtagcttta gctgcaatgg
    10381 ttgaattagt tgaaagtgga tttggagaat agatataata attttttaaa aatttgatat
    10441 ttagaatcta atattactta aagtaaagta acagagaagt attaaacata tacagaccaa
    10501 catttgtaga tatttaattt ctttgttact ttcatgttta tttaatcaga agattaagac
    10561 tattgttaat tgggtataaa aatataaata ttttagaaaa tgaaataaga tatttttaat
    10621 cataaattca gagtaaaaat acagtaacaa atagtttata aaattttata aaaaattata
    10681 agtaaataaa actataaaaa tgtaaattat ttatataaat ttataaagtt tatgtgctag
    10741 atgatatttt ttaggaggta gagttatggc ttacaaagga ataggtgctt ctcctggtgt
    10801 tgctttaggg aaagcgttag tagttgaaca tagtgaatta gtaattgaaa aaaaatctat
    10861 agataatgtt gaagctgaaa ttgctaaatt agaagatgca gttgcagttt ctaaagaaga
    10921 attagtaaaa gttaaggaaa aggcttctga agaattagga gcagaggaag cagaaatatt
    10981 tgaagcacat ttattagttt tagaagaccc agagttaatt ggttctgcaa tagataaaat
    11041 taaaactgaa agtgtaaatg cagagtatgc tttaaatgaa ataaaagaga tgtttgttag
    11101 catgtttgag tctatggaca atgagtacat gaaagaaaga gcggcagata taaaagatgt
    11161 tacaaataga atacttagac atatattagg aattaaagtt gttgatttat ctgctttaag
    11221 cgaagaagtt gtattaatag cacatgattt aactccatca gacacagcaa ctatgaataa
    11281 aaaaatggtt ttaggatttt taactgatat aggtggaaga acttctcata ctgctatcat
    11341 gtctagaact ttagaaatag ctgcaatcgt tggattaaat gatataactt caaaagttaa
    11401 ggatggagat tttgtggttt tcaatggaga cacaggtgaa gtaatcgtga atcctgatga
    11461 agagactata aataaatata ctgaattaaa agctaagtat gaagatgaga gaaaagcatt
    11521 acagctatta aaaggaaaac catctgtaac tttagacgga aaacatgtgg aattagctgg
    11581 taatatagga acacctaacg atatagaagg tctaataaag aatgatgctg aaggtgttgg
    11641 tctttataga acagaatttt tatacatgga tagagattct ttcccaacag aagaaataca
    11701 atatgaagca tacaaagctg tattagaagg aatggatgga aagcctatag ttataagaac
    11761 attggatata ggtggagata aagaattaag ttatttaagt atggaacctg aaatgaatcc
    11821 attcttaggt tacagagcaa taagactttg cctagataga aaagatatat ttaagactca
    11881 attaagagct ttatatagag ctagtgttca tggaagatta agaatcatgt tcccaatgat
    11941 ttcatcttta gaagagttgt tacaagctaa agaagttgta aaagaagttt tagcagaact
    12001 tgattctgaa ggtgtggctt atgctaaaga tgtagaaatt ggtatgatga tagaagttcc
    12061 atctgctgct gtaatatctg atgtattggc taaacacgta gatttcttct ctataggaac
    12121 taatgattta attcaataca cttgtgcagt tgatagaatg aatcaaaaga taagctatct
    12181 ttacaatcaa tttaatccag ctgttttaag acttataaag actgttatag acaatgcaca
    12241 taaagaaggc aaatgggcag gtatgtgtgg agaatctgct ggagaccaaa aaatgatacc
    12301 aatactttta ggtatgggat tagacgagtt ctctatgtct ccaatatcaa tacttcctgc
    12361 tagaaagtta ataacttcag ttaaagagtc agatatgaaa aaattagctg atgatgtact
    12421 taatatgggt actgctgaag aaataaaaag ctatatagaa aagactttta atatataatt
    12481 aatgttatat agttactaaa attcattttt ctaaataaca aaattatatt ttacgaataa
    12541 aaaaactata gtatatcata tatactatag tttttttgtt atataaatta cgtatcaaaa
    12601 tttgtaaaat gaatagatat aattactaaa tatgtgatag tattggctat aataggatat
    12661 aaaaaataat attgtaaaaa aaagtttata tttagtaatg aaaatttaac gttttacttg
    12721 aaattatgtg ttttgtaatg tattattaag gctctaatag gaacattttc atcaattttt
    12781 ctatagtggg ggtaggaatt atgaggtaca aaaaaagaaa tatagataag tcaagtgaac
    12841 tttcatttca atctctagca aaatatttta taactttcta tatagtaatt atattaatta
    12901 ttctttctat gttttgtata aatacaatat gggttatgaa aaaagataat caagatttat
    12961 tatctacaac atcaaatcgt attgaagata gggtaggaga aacaattaag ctattgaact
    13021 ctttgagtaa agataattac ataatagata atagtgtgaa tggcatagat aaaagtaaag
    13081 aactttcaat gtatagtgat gaatttgggt atatgatgat atgttttgtt gataagaata
    13141 taattgtgtg ggatgaagaa ggtggtaata ttagtctagc tggtcgtgat tatatgcaga
    13201 aactttttgc aacaaaaaaa tatcaagtta cagatagttt tgcagcaggg gctgatggaa
    13261 aaactttaaa ctacactatt gcatcaccta taataaagaa tggaaatgta gatggttgtg
    13321 tatttgcagc aatttacttt gatgagataa aggatatact caaacaagga aaaggtcaac
    13381 agcaacagaa tttcatatta tttggaagtg aaaatcaaat aatgaattta acaagtgatg
    13441 aaactgcata tggagagaag tatatgaagt tatcgcagaa aacaaaatat cttggaacca
    13501 atgcaaaaaa agtagaaaag gatatgttta ataataaatc aggtgattat tgggcttttg
    13561 aaaatggaaa attatacttt atgtcctata aaccaattaa gaacacaaat tggagtttat
    13621 tttgtaaagt taatttctta gatatttttg cattaaattt aattcctatg ggagctattc
    13681 tatctgttat aactttagta tttattattt tgatttgtat ggagaaaagc ttcatgaaaa
    13741 aacgaatgga agttattgat atgcttttag gttctgttca agaattagaa aaacgtatat
    13801 atcaaacaga aagacctagt gaagtagact ttaaagaaat tattcagctc acaagtaaag
    13861 gtctgactga tggattaact ggtgttgtaa cacgtactgt attttttgac caaatttata
    13921 atcgtgttga aatggaggaa aatgataaat tatctgtaat ttcatttgtt gatttagatg
    13981 atttgaaatg tattaacgac ttatatggac atgaaattgg ggatatggtt ttgaaaaatg
    14041 ttggatatgt acttcgtgaa tatgagataa aatataatgg tttaattggg cgctatggtg
    14101 gagatgaatt tgttatgttg ttatctggtt ttacatcgag agaagaggtg gaggatttat
    14161 tggatgagat tgctattcgc ttacatactg aagtactaac agatgatggt atcattcaaa
    14221 ctcattgtag tgttggagct gtagtatgta gtgtaaaaga caataaagat atagataatt
    14281 tattaaaaca ggctgatgaa gctttatact atgttaaacg taatggaaaa ggtaattatc
    14341 atttagttta atgtatgtgg aaggagttta tatcatatga aaaatgtatc taaaaaattt
    14401 tttgtatgta ctattattgt attaataaca tttttatgtt ttagtaaatt tgctatttat
    14461 gtttttgaca aacaagaaaa gaaagaaaca aaagtagtag aaaacatgtt aaatgtctac
    14521 aagaataaca ttattttaaa tctgactgaa aaatttaatt atagcaaaga cttgacatca
    14581 gttttagttt caaacaatta ttctatagat aaattaaaac ctattgcaga tgaaattctt
    14641 aaaaacaatt cttgcatata tatttcagtt gtagaaaaat ataatgtagc tgcatttttt
    14701 tctaattctg tcaaaaaaga tattatcaat actccaatac aggaactgaa ttattcttat
    14761 tcattagcaa attctatcaa aggattagtc gtagaaggtc cttctaaaga ccagttttca
    14821 aatggagatg tgtttttaat tactaatcct atttataaaa atggagagta ctggggagaa
    14881 attacagtag ctttagatgc caaaaaagtt gtagattctt ttaacttaaa taaattaaaa
    14941 gaatctggat ttgagtatga actttggcgt gtacacctag ataatggttc aaaatctaca
    15001 atagaaattt ctactaaaga ttttaatttt tccaaatcac atgaaattaa gtttaaactt
    15061 ccgactgttt ggactcttag cattgtgtac aaaaatggat ggaataataa gaatatagat
    15121 tttattgtat ttgttatatt gttaattatt tctatattaa taggtgtgat gacatttatt
    15181 tacattgaaa agaagtcgat tagtaaggaa ttaaatcaac agagtcttaa aaatttagaa
    15241 actggtattt tgaatagaga aggtttttta attaagttgc aagagcatct gaatagaaaa
    15301 aataatgaaa tagtgttgct gtatatatgt attactaatt atagtaaatt tgctccagtt
    15361 atgagctttg aaaaaagaca agattatctt acacatatag taaaaaatct agataattac
    15421 ataaaaagtt ctcattttat tgctcgtata agtgaggaag gttttgttct agctttagaa
    15481 gaaaattgtg ataaaaatga attgcttaac attgaaaaag ggatttcctt agagctttta
    15541 cgaaaaatta aagtagaaaa taaacaagta tttttaacga ttggttatgg ctcatctagg
    15601 tgtgttgatg atagaaaaga tgcagaatct ctgttacttt cagctattga agattatgag
    15661 agaagatttc cagcaatgtc tcctattcat gattttacta aaaagtgtga agatatgctt
    15721 gatgaaaaga aacagattgt atttggtgaa tatccagatt tagaaataaa caagttgtct
    15781 aaagtaatat acaaatatta taaaaaagta cagcgtatta tgtataaaga ttataatatt
    15841 gatattggaa ataggcgagg gtatatgcgt gatgtcgaag tattaattga ttataattct
    15901 gagagaatgt tatacttatt tttaatagac atatgcaagt ttggaaaatt taatgaactg
    15961 ttttctattg aaataggaga tgttatttta aaagaagtta gtaacaaatt aaaaacaatt
    16021 ttttctaatt atctatatcg tattaatgga gatgtatttt taggcatttc aatagataaa
    16081 gaatcttact tagatacttt acataaggtt aatgaagtat ttaatagttc tttaagtgta
    16141 gaagatgtac tttttgatgt aaaatgtaat attggtattt gtcaatatcc atatcatggt
    16201 gaaaatccat atgaccttct ggaaaaggta caaattgcat tggagtatgc aaaaaaggat
    16261 gtcaaaagta atgttaaagt ttatgataag gaattgacag aagagattta ccatgaaaat
    16321 aatattatta aggatttaaa aaaaggtctg gaagaacaaa cactagaagt ttggtatcag
    16381 ccaattttta atgttagtaa aaactgttat ataaaagctg aggcactaat tagattgaga
    16441 aataaagagg gcggttatta ttctgcctat gaagttatta cttatgcaga aaagcatgga
    16501 cttattaagg atgtaggtct atatgtacta aaaaaggctt gtgatgtaac tacaaaaata
    16561 aaagataaaa atatagactt tgaacaaatg caattaaatt tatctgctca gcagattgta
    16621 cataagcagt gtgcaaatga catattatca tgtattgaga attgtggtct agataaaaag
    16681 tatatttctg ttgaagttac agaaaaagta cttatggaaa cttttgaaat tgtaaatgtt
    16741 actcttgata aactaaaaaa agaaggcatt agcatagctt tagatgagtt tggttctgga
    16801 tttagtagta taaattatct ggcaaattta cctattgaaa atttaaaaat agataaggaa
    16861 ttagtagaac aaattaccta ttctaaaaaa caatttgaat tattaaaaac tatagttcaa
    16921 attactaaga ttaatggcat ggatattgta gcagaaggtg ttgaaacaaa agaaactctg
    16981 gatttagttg taacttcagg tgtgaatttg attcaaggtc attatttttc agaaccttta
    17041 agtgaagata aatttattga acttatgatt gaagaataaa ggttaataaa ataaagattt
    17101 aaggtattga aaaataatta aaagtattta ataatagaat tagggaagaa taagtttata
    17161 agtaaaaaca attgattagt agttaataaa ataaatgtga tatccaaata aatcctaaat
    17221 ctaatttgag ttttaatata gatagtctaa agtttgatta ttaaagattt aattgtaagt
    17281 tgttacagtt gatgatattg attagagatt aaagtttaga gtagaaagtt accttaaatt
    17341 taaaaatgca agataaccca atctaaaaat tataatcact cgataggttt aaggatttat
    17401 ttgtttatta taaataggag ggtgtctcaa aatgaacttt ttagtttatg aggcactctc
    17461 ttttatatag aattaaatat attttattat ttcaacaatg aaaaagaagc ttatctctat
    17521 tttgagacag cctctttgta tactattatt aatttaatca ataacataat ttaaaattat
    17581 aatactagtt aagatttcct gctttattag taaataattc tttaatagca ttgtttctgg
    17641 aaactaaatc gttatcagag ttgtttgttt ttttcataat cactgaataa cgttttttat
    17701 ttataaaaaa tattaaaatc ttatcatcat caaaattgta ttctttatat ctgtcgatag
    17761 actcataacc tagatttaga atgtttttgt cttttatact atctacatct aaaatatatg
    17821 atgggtcagc ctttagtaat atattaatta aacatttttc ttcaccagca cagaataact
    17881 ccatcttact tgatatagat ttagattgct ctgaagttat ttttgcagga ataaggttgt
    17941 cattatcatc tcttagaaga taaccatgtg tgtcttgttg atatactaaa ttaacctttt
    18001 tcttaggaac actaatcatt ttatctaaat gtacataatg atgattttca acaaaacctt
    18061 catttaatat ttcaacaaag ttattaattt tacctaggca aaaagattct atagattctt
    18121 tacagaattt atttgtaaat ggtattatgt ttacgttttc ctcgtctgta ttccatgcta
    18181 tagcataatc caacgaatat acttcttcct ctatatctgc aaaattatat tgtactatgt
    18241 ttcctttcaa aattactcga cctccatctt ataatatatt taatatactt tataaaactt
    18301 atattatatt ta
//
LOCUS       DAEMGG010000078        18135 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEMGG010000078 DAEMGG010000000
VERSION     DAEMGG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.078"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(82..768)
                     /locus_tag="KPK24_002444"
     CDS             complement(82..768)
                     /locus_tag="KPK24_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG2300112.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(817..1350)
                     /locus_tag="KPK24_002445"
     CDS             complement(817..1350)
                     /locus_tag="KPK24_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG2300113.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(1394..2026)
                     /locus_tag="KPK24_002446"
     CDS             complement(1394..2026)
                     /locus_tag="KPK24_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG2300114.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2116..3195)
                     /locus_tag="KPK24_002447"
     CDS             complement(2116..3195)
                     /locus_tag="KPK24_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG2300115.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3328..4674)
                     /gene="pepV"
                     /locus_tag="KPK24_002448"
     CDS             complement(3328..4674)
                     /gene="pepV"
                     /locus_tag="KPK24_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG2300116.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4743..6023)
                     /locus_tag="KPK24_002449"
     CDS             complement(4743..6023)
                     /locus_tag="KPK24_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG2300117.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6150..6410)
                     /gene="hfq"
                     /locus_tag="KPK24_002450"
     CDS             complement(6150..6410)
                     /gene="hfq"
                     /locus_tag="KPK24_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG2300118.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6524..7459)
                     /gene="miaA"
                     /locus_tag="KPK24_002451"
     CDS             complement(6524..7459)
                     /gene="miaA"
                     /locus_tag="KPK24_002451"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG2300119.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7497..9464)
                     /gene="mutL"
                     /locus_tag="KPK24_002452"
     CDS             complement(7497..9464)
                     /gene="mutL"
                     /locus_tag="KPK24_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG2300120.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9475..12318)
                     /gene="mutS"
                     /locus_tag="KPK24_002453"
     CDS             complement(9475..12318)
                     /gene="mutS"
                     /locus_tag="KPK24_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG2300121.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(12479..13213)
                     /locus_tag="KPK24_002454"
     CDS             complement(12479..13213)
                     /locus_tag="KPK24_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300122.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(13182..14195)
                     /locus_tag="KPK24_002455"
     CDS             complement(13182..14195)
                     /locus_tag="KPK24_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2300123.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(14217..14987)
                     /locus_tag="KPK24_002456"
     CDS             complement(14217..14987)
                     /locus_tag="KPK24_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300124.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSIHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(14962..15252)
                     /locus_tag="KPK24_002457"
     CDS             complement(14962..15252)
                     /locus_tag="KPK24_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG2300125.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(15383..15482)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      16102..16191
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16244..16420
                     /locus_tag="KPK24_002458"
     CDS             16244..16420
                     /locus_tag="KPK24_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300126.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(16535..16891)
                     /locus_tag="KPK24_002459"
     CDS             complement(16535..16891)
                     /locus_tag="KPK24_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2300127.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            17147..18060
                     /locus_tag="KPK24_002460"
                     /pseudo
     CDS             17147..18060
                     /locus_tag="KPK24_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
BASE COUNT         5820 a         3066 c         1938 g         7311 t
ORIGIN      
        1 attttccttc taaataaaaa atttgtctaa agtttctaaa aaatttagac aaatcttagg
       61 ttattactct ttaaattatt tttaaattat tccaaatacg ccaaataata ttggtataaa
      121 tatagctggt aacaaattgg ctacttttat attcgttaat ttaagtacat ttagaccaag
      181 tcctataatt aataaacttc cagctgctgt catttctgtg actacaggat cacttaaaaa
      241 tccatttaaa aatgatgctc ccacacatat aataccttca tacaaaaata cagatattgc
      301 agaaaacatt acacctatac ctaaagaagc agtaaatata attgaagcta ttccatctat
      361 aacagatttt acaaataaag tatcatgact tcctgtaaga ccactttcta atgaaccaac
      421 tactgccatg gctcctatac agaataataa actagatgat ataaaaccct ctgctataga
      481 atctttttta tttcctttag aaaatttact ttgtaaaaaa gcacccaact tatttaccca
      541 tttgtcaata tctattaatt ctcctatcag agcacctaca gctacggata ttattgtcac
      601 taatgtgttt tttccttcta gtgctcctga tataccaatg tacaatacac ataaagcaat
      661 tccattcatt atagtatcgc ttactctttg aggtaatcct ccttttatta taagacctac
      721 tatacatcca cctgcaattg ctagactatt tgctattact ccaaacattt tttctcccct
      781 cgattagcta attgaaattt atcctattat taaaacctaa acatctatac tttgtattgg
      841 atattttaaa tattccaaaa attcttcaaa accttttcta gatatattta cagttttagt
      901 atttatgtta ggatgaaaat ttaagtaatc ttctttaatt acatctttat ctatgtaaag
      961 ctcaactttt ctttctgtat catttattaa tgaaaaagga tttacacttc caggaagaag
     1021 tttaagtaca ttgtatagtc tttcaggtga agcaaacgat aatctagaac ttcctatctt
     1081 atctttaagt gagtttaaat caacttgttt ttctccttta actacaataa gataatactt
     1141 atttcctttg gcatttctta aaaataaatt tttacattga cacccctttg ttattccatc
     1201 taaaatgact aattgctctg ctgtatatac agcttcatgc tctactacat catattcaac
     1261 atttaattta tccaaaatat catatatttt agtttcattc atgctagatt cattttcatc
     1321 ttgttctttt ttattattta aatcactcat acttttctcc ttaaacactt catcataata
     1381 tcattgtgag gtattattct actatagcta ttgctcttac tggagctcca tcagcatcat
     1441 tatacttaaa tggtgctgct ataaatagaa atttttctgg aacattttct aaatttgtaa
     1501 gattttctat gattattttt cctttttcaa ataaaatatg atgcacttca aatactgatg
     1561 tgtcatatct atccactgat agcatatcta ttcctatgcc ttttatatta gtattagcta
     1621 tatagtttgc agcttctttt gttaatgtag gataacctac atagtactgc tttttatccc
     1681 aaaattttga ccaacctgat ttaaatatta taaaatcact attttttatt tcatcttcat
     1741 atttttttag atattctaat tctatattct catttccctt ttcaagttcc aatacataag
     1801 cttttccaac aaaattttct atatccaact tatctagggt ctcaccttta gtatacatat
     1861 gtcttggtga atccatatga gttccattgt gagaaaatac actaattaat gtttcttgat
     1921 aaccattttc ttcaataata gctactttct tgatatctgg tctattaggc tctgcatata
     1981 caggcatgtc attatgtgtc acatgagtta aatcaaaaac cttcatcttt atataccccc
     2041 tatgttttta ttttctatta tatacctaaa ttcattttat ttctatcaga ttttattcca
     2101 ttatatacca tactactata tattttttat aaacctcaag ggatttaccg aaaaatcttg
     2161 gaaagaatta tcagtaaata taacatcttc acccaaaaaa cgtttatacc ttccttcttc
     2221 tttctttaaa gtaattctct gtttttgtga atgattatta aaaaccatta tcttaagagg
     2281 aggttttttt ctattagtct gcactctttt taacatgtat tttatgtgca tatcagcttc
     2341 aggaaatgaa tatccacaaa atattaaaag gtcagtatct cttaaagttt tttctgtttc
     2401 attccaaaca ttacttaaaa atatgtttga catattctta aaatatgttg gtggcactat
     2461 aataggttct gttaactcac cacactctaa acattttgtc tctgaactat tttcaataag
     2521 cttcatgaca ccaccttcat gtggtgtaag agttacacta ttacaaatgg ggcaataaag
     2581 ccaattcaat gacccatgta tcttatataa tttgattaat ggttcttttg gttttctcca
     2641 atttaactcc tctttaaaat ttgtaaactc cacaccataa tcaagcataa ttggcaaatc
     2701 attcttatct cttaatccag taactgtatt gtctatatgt atatcataat ttgcactaat
     2761 aaaagtagta tcaagaagca aatcattttc caaaagattt tcaactaaaa gtttatgata
     2821 tttattatct gttctaggtg tattatgaat tgcttcagcc attagcagta ttaaatattg
     2881 tcttaaaaaa cgtatactat cacttctgtt atttagattt tctagtccaa aatttctaaa
     2941 tgcttctttt ctttgttctg ctaagtctaa aagacctaaa acttcttcaa aggtaggaaa
     3001 atttgcatta tctatattat cttttactat atcaatattg aacatctgtt taaagaaaat
     3061 atacaattct ctattcattt tggtattata atttgtggga tttagttttt taaaatattc
     3121 gctaaaaagt tcattttgaa taggtagatt ctctgctgct gaagctcctg ctcctaaaaa
     3181 tattgctatt ttcatatagt tcctcctaat ataaatatta ctcctatttt tatatccatt
     3241 aaatatccat atcatttatt ttatgataca acaataaagc tgtcttttac tagaaaaaat
     3301 cctattacaa gacagcctta tattatatta aatctttaat aattcgtaaa tagaaagagc
     3361 atatatttga gtagctttta taatatcctc tactattaag tattcattag cttggtgttc
     3421 tgtttctggt tttcctggaa aaatagctcc aaatgcaaca caattatcta aagctcttgc
     3481 gtatgttgct cctccagatg aaagtggtgt tccatcaaga ccagtttctt cttcatatac
     3541 ttttctaagt gtcttaacaa gaagagtatc ttctggaaca tagattgaat ccaagaaatc
     3601 atattcttca taatttaaat tgtatttatc tgtcattttc tttaactctt ttacaaaatc
     3661 atcctttttg tatgttactg gaatccttac atctattcct gcaaattctt tttcgttatc
     3721 aatagttact ctacctatat ttactgttag tttacctgat acatcatcct tgcaattagg
     3781 aattatattg tttccattag catcttcacc aataacttct gctaaaaact taattatatt
     3841 agaatctata cctatattat taagagctat acaaagtcta gccactgcat ttataccagt
     3901 atcacttgct gctgaatgga cactttttcc aattatacag attttattac cttcaacagt
     3961 atactcaaaa tttaatttat caagttcttt ttttaattta tcagagtatt ttccaatata
     4021 gattgcttta cctggaactg catttaaagc ctttccaact gacaactcta tatcactttt
     4081 gctatcacaa gtcaaatgaa cttgtaaaag acctttctct gcatttgtta ttgggaatct
     4141 agaatctgga gtaaatccat agtttggtat ttcttcatta ttttctttat atttatttat
     4201 acatctccaa agattttctt catcagcccc aaaaatgaat cttatcctct tattaaagtc
     4261 tacatttaaa tccataagag ctttcactgc ataaattgct gatattgtag gtcctttatc
     4321 atcttgtgtt cctcttccat acaatttacc atcttctaaa acagcttcaa atggagggta
     4381 attccagctt tctaaatcac cttctggaac cacatcaacg tgaccaagta tacctatcat
     4441 tttttcacct tgtcctattt ctgcatatcc ataatatcca tctttgtata ctgtcttaaa
     4501 tcctaaagat ttacataagt ctaatatacc tcttaaagct ttatctacat tttcaccaaa
     4561 tgggcaattt tcagttgctt cagatataac actaggtatt tttacacttt cttgtattga
     4621 agaaatcatt tcatcttgta gagaatttac tttttcttta atttgctctt tcatattatc
     4681 acccttcaat tttcatcata atatactaat aaatattttg accttttaaa aaaaaaatat
     4741 gcttattttt tcagtttttt tataaattca atcgctttta aagtccccat ttttgaatgg
     4801 tcaaatgtaa gtccaccttg aaaataaaca ttataaggag gtcttattgg agcatctgca
     4861 cttaattcta tagaagaacc ttgtataaat gctcctgctg ccattatgac ttcactttcg
     4921 tatccaggca tgtcccaagg aacaggtttt acataagaat cgactggtgc agcttcttgt
     4981 ataccttcac aaaaacttat tacttcatca gcattattaa gtctaataca ttgaataata
     5041 tcacttctta agtcatcata ttttggtaat acatcatatc ctaatttttc aaaagctctt
     5101 gagcaaaaga tagctcccat tacagcctga gatactatat aaggcgccaa gaaaaaaccc
     5161 tgaagtacat ttctagttgt tccaaaagta agaccacatt cttttcctat tccaggagaa
     5221 gtcattctat aagatataag ctcaatcaaa tcttttctac cagctatata tccacctgtc
     5281 aatgcaagcc caccaccagg attttttatt aaagagccag ccataacatc tgctccaaca
     5341 tcagtaggct ctttggtatc taaaaactca ccataacaat tatcaaccat tactatagct
     5401 tctggtttaa cagactttat tacttctata gcttctttta tatcactaat tgaaagtgat
     5461 tttctccaag aatatccttt tgacctttgt atcataacga gtttagttct atcatttatt
     5521 ttgtttttaa ttccttctaa atctaagttt ccatcctcta aaaaatctac atcatcatat
     5581 gtaacaccat actctttcaa tgaacctttt tcttctctta tgcctataac tccctctaaa
     5641 gtatcatatg gtctaccagt aacagataaa atttcatctc ctggtctaac tataccttgt
     5701 atacataaag taagtgcatg tgtaccattt acaattattg gtcttacaag agcatcttct
     5761 gtattaaata ctttagaata aatttcctct attttttcac gaccaatatc attatatcca
     5821 tatcctgtag tccagttaaa gtgcatatca cttagtttag attcttgcat agcttttaaa
     5881 actttatatt gattatattc tcttatttct tttatttctt caaacttatc ttttatttct
     5941 tccattattt cttgcgatag tttaaaagta tcatcatcta ttccatagta gtcttttaat
     6001 aattcttttg tctcattaag cattttatca cctgtctctt tattatttta tttaaacatg
     6061 taaaggagac tattttacta aaacaaataa attatagttt ataagttagt ataagtctcc
     6121 tctcaatcat cttaaattaa attaattatc tatctgttgt tattattatt gttgttttgg
     6181 ttttgatttg ttagattaat atactttcca ggttgtatag ttgatacagc atgtttataa
     6241 atcatgtttt gttgtctatt atccccttct attaatatta tataactatc aaaccccttt
     6301 acaagccctt taacttgcac tccattaact aaatatatag taacaggtat cctttctttc
     6361 cttgcattat ttaaaaacaa atcttgtaaa tttaaaactg tatttttcat tagatttgta
     6421 ccctcccaag attaaattga acttctaaaa tattcctacc ttatattcta tataagttat
     6481 gacaaatttt caatattaag ttgtatactt aaaaatttat atattatttt atagaatctt
     6541 taatatataa gattatttca ttttttaatt cgtctatatt ttcatattgg tctaaatcaa
     6601 accattttgc tgttttatat cttttaaacc atgttatctg acgttttgca taccttctag
     6661 aatctctttt aattatttca atagcttctt cgtatgtata ttctccatcc agatacttta
     6721 atatctcttt ataacctata ccttgcattg atatcatatc ttttttaaat cccatactta
     6781 aaagtttttt tgcctcttca accaatccat ttttaatcat aatatcaact ctcatattta
     6841 ttctttgata caaatgttct ctatctctat ttagaactat tattattggt tgatattctt
     6901 cattaaattt taaatcactt gaaaaatcat tcattttctt accacttaga caaacttcaa
     6961 gagctcttat gaccctttta gtattgtttt tatggattct attagcagat tcttcatcaa
     7021 gttcttttaa cttattatgc atgtaatcta ttcccttttc agcaagctgt ttttgaagtt
     7081 cttctcttaa ttcattattg gcatcagatt gtgcaaaatc catattatat attaaagagt
     7141 ttaaataaag tcctgtgcct ccagttacta aaacacattt ttccttttca tttatttcat
     7201 gtatatattt ttttgctctt tgttgaaact ctgaaactga aaatggatag tcaggcttaa
     7261 cttcatctat aaggtaatgc ttcactcctt gcatctcttc ctctgtaact tttgcacttc
     7321 caatatccat atattcatat atttgcatag aatcagctga aattatctct gcattcatat
     7381 cttttgctaa ttttattgac aaatctgttt ttccaacagc agttggacct gtaagtatta
     7441 taagtggtat ttttttcata acattccttt ccatatattt taaatatcca aatattttac
     7501 ataattcttt taaacatttt ttctatttct gtcttagata tttcgaccat tataggtctt
     7561 ccatgaggac atgtaaatgg attctcacac ttttctagct gttccaataa actttttatt
     7621 tcaatatcgt atattttatc atttgctttt atagcagacc tacatgccat agatgcaaat
     7681 ctctctcctt ttaaatcata attacttgta atttcttcta tattatcaat tatttgaagt
     7741 ataaatttct cagtttcagg tactccaaat atagtaggaa cacatcttac cattatatga
     7801 ttattgccaa aaatttctag ctcaaaacca aatttcataa ataactccaa gttattttca
     7861 atttgaagca tatctatatt tgatacttca ataaccactg ggtctaaaag tatttgcata
     7921 tttatatctt gtctatagaa cttctccata tatctttcat atagtattct ttcatgtgca
     7981 gcatgttggt ccaacaaata catagaatcg tctttgctca aaattatata agtattaaat
     8041 acaactccaa ttacaaaata tccgtataaa gaaaacttgc gttttgaact acacaagtat
     8101 tcttcttcac tgtccttaat actatctcct aagtaataat ttccttcatt tttaattcca
     8161 tcaacttgaa actcttcttg tatattatca tttgcactat cttcacttag ataattaatg
     8221 ttttttacat catcttctac acttgcattt aggacttcac ttactgattg aaattcattt
     8281 attggttttt cacttgataa agtaacaact tcaatgactt catccttacc tttagtaata
     8341 ttactatttt taggagtaga ttctaataaa tcattatttc ttaatttata atctgtactt
     8401 ttttctctac tgttaatagc gattctaggt tgtgtttttt tatcactata agtagcatat
     8461 tttccaataa gatttgagtg tattaattta acctttaaaa aatctctaag ttctatatac
     8521 acttcttgtt ctttttcaaa tttaatttca agtttattgg ggtgtatatt tacatctata
     8581 catgatgggt ctacttctat atttaagaag cacactgcat gtttacctat tggaattata
     8641 gacttataac tttctgttat tgcatctatg attattttac tttttacaaa tcttttattt
     8701 atatagatat gttgtaagtt tttatttgaa cggtatatat tgttattacc tatatatcca
     8761 ttcattttaa agtgattgca cttaaactct acatctatta tattttccgt tatttctttt
     8821 ccatatatac ttctaatagt gtttacaagt tttccatccc ctggagtatt tagcatctgt
     8881 ttattgttat ttgtatattt aaattgaatg tttggattac ctattgcaag tttatttatc
     8941 aaatcactta tgtttatagt ttctgcatga gtagatttta aaaatttctg tcttgctggt
     9001 gtattaaaga aaatatcttt tataatgatg gtagttccat tagtagaacc tattggttct
     9061 ttcgatatta tttttccacc ttcaacatat attttagttc ctattatttc atctttagtt
     9121 ttagtcgtca tttctaattt tgatacagct gaaatagatg ccaatgcttc tcctctaaaa
     9181 ccaagagagt ataaatcata caaatcatca atttttttaa ttttactagt agcatgtctc
     9241 aaaaatgatt tttcaacttc acttgaaggt attcctattc cattatctgt aatcttaatt
     9301 agactttttc ctccatcaat tatatctatg gaaattttat ttgcacctgc atcaatggag
     9361 ttttcaataa gttcttttac tacagaagaa ggtctttcta caacttcacc tgctgctatc
     9421 ttatttatag tcaaatcatc taggatgtta attatatttt tcatttttac acctctaaga
     9481 ttttatttct tttgcttttc tttgaagatt ataaagagag tttatagcat ctaatggagt
     9541 catatttaaa atatccagat ttaaaatttc ttctgatagt atatctctat ttacactatc
     9601 aaaagagatt tgagttaagg ctacttcttt tacagaatct tcttgattaa ttttctcttt
     9661 atcatcattt tgcttcatta aagcaacttc atcatttaat gctttaaatt tagaatttaa
     9721 tttatcataa tcctttttta tatgtttgta gtcttttact ataagttcat gttctatttt
     9781 tagagattca tatttattct taaaattact ttgatttacc ttaactaatt cttcgtccaa
     9841 attactatta attatattat tgttttcttt atctccattt attgcaacac tgttgtaaac
     9901 atgatttttt tccaagtctt taagtatata ttttgctctt tctatgactt cgtctggtaa
     9961 tttagctaat ttagcaacat atattccata actcttgtct gctccctgag gaattatttt
    10021 tcttaaaaat attattcctt ccccatcttc tttgactgct attgaataat ttttaacctc
    10081 tttaaactct tcttctaaat cagtaagttc atgataatgt gttgcaaata atgtcttaca
    10141 tctaatattt ttttgaatat attccactat tgaccatgct aaacttatac catcataggt
    10201 actagttcca cgacctattt catctaatat aactaaactt ctctctgttg cattttttaa
    10261 tataagggat acttcgttca tctctaccat aaaagtagat tgaccttgag ataaatcgtc
    10321 actagctcca actcttgtaa atattctatc taatattgga atatcagcag actctgctgg
    10381 tacaaatgag cctatatgtg ccataagagc tattatagca gtttgcctca tatatgttga
    10441 tttacctgac atattaggac cagttattat gttaataatg ttttctccac gatttaaata
    10501 agtatcattt ggaacgaaat tttcctctcc tactatattt tcgacaacag gatgtctacc
    10561 atttcttata tcaagtttat tattttcatt tattgctggt tttacgtagt tatttatatg
    10621 tgcaactgtt gcaagggaca caaaaacatc tatattagct atagtcttag ccactttttg
    10681 tattctatct atgtttttat atattgtatc tcttatctct acaaatatct cgtattctaa
    10741 agatttaatc ttttcctctg catgtagtat cttttcttct atttccttta attctggagt
    10801 tatatatctt tctgcattac taagtgtttg ctttcttata tatgtctcat ctagctttgc
    10861 ctgtttaaag ttggcttttg ttatttctat ataataccca aaaactttat taaatcctat
    10921 ttttaatgat ttaactcctg ttttttctct ttccctgttt tctatttcct ttacaagaaa
    10981 ggctccattc ttagaaatct ctcttagttc cttaagttca tcactaaaat ctgatttaat
    11041 tatgtttcca tcttttatgg ttatcgtagg ttcttctaga atagcttcat ctattaaatt
    11101 gtatatatca tctaatttat ccatttctga aacatactct tttaggattt ttgcatttga
    11161 taaatttata gtatctttta aattaggtaa cttttctatc gaatttttta agtgaattaa
    11221 ctcttttggt gttactctct caaatgctat ctttccacat attctttcta tatcgtatat
    11281 atttttaagt atatcattta ggtcttctct taatataaag tcatctttta tttcttctat
    11341 tacatctaat ctattttcaa ttttagattt atttatcaaa ggttcttcta catattttct
    11401 taaaagtcta cctcccattg ctgtagaagt tttgtcaagt acatgtaata atgaaccttt
    11461 tttcttattc cctcttatag tttgagtaag ttctaaatta gttctagtaa acatatctag
    11521 aaccatatat tctgatgaat tgtatatatt tatattatta atgttagatg ttatttgttt
    11581 ttgagtattg tatatataat tcagaagaat tgataagctt gactttatta gacctttatc
    11641 atcaaacttt agtttttgta gataaacatc tggaaaatat tcttttaata tatttatatc
    11701 caaatagtta tcactaaaac tttcatttat gtatatatta cttacagtag ctatatctct
    11761 aagcttttct ataaagtcta aatcatttat aattatttca gttggatgga tttttgcaat
    11821 ttcttcaatt accttatctt catttaaaca tgttgcatta gtttctcctg tacttatatc
    11881 aacataagtt agacctatat tagtaccatc cttatacaaa gataataaat aattattctt
    11941 tttattttca agtaaatttc catctaaaac tgtaccagga gtgatgaccc ttataacctc
    12001 tcttctaaca attccttttg ctgtagatgg gtcttccatt tgttcaccta ttgctacttt
    12061 atatccattt tcaactaatt ttgatatata tgaattggct gagtggaaag gaacaccaca
    12121 cataggagca cgttcttcta aaccacaagc ttttcctgtc aatgctattt caagagcttt
    12181 tgaagctact aatgcatctt caaaaaacat ttcataaaaa tctccaagtc taaaaaacag
    12241 tatacagtct ttatatctat tttttacttc aagatactgt ttcatcatag gagttaattt
    12301 attcatatct atttgcatta tcaaacctcc ttcttttcta attaaatatt atatcatagt
    12361 agatgattat tattaacaca atatataaaa ttttgacata taactaaaaa caatccaaaa
    12421 tgtatactca cattttggat tgctaagttt aatgcttatt aaataaaaat attttttttt
    12481 acaataaaga tattatttct tttttaagtt taatgatatt ttcaaggtta tcttcattta
    12541 actttctact atctataaca aattctttct ttactattga tggttttttt gaaataacat
    12601 aaattctgtt tgatagcatt atagcttcgt caatatcatg agttataaga agtattgttg
    12661 aatttacttt tttcttaaca tctaaaagcc atttttgcat ggatgtttta gttaatgaat
    12721 caagtgcccc aaaaggctca tctaaaagca ttatgtcatt agaatttata aaagttctca
    12781 agaagttagc tctctgtctc attcctccag aaagttctga aggatattta tcttcatatc
    12841 cgcttaatcc aaacacctca aaataaggtt ttaccattga cctagcttta ctcttttttt
    12901 ctttttttaa aattagtggt agtgacacat tatctattat agttttatat ggcaagagta
    12961 aatctttttg atacatataa ctatacttac catttatatt cactttgcca ctatcataat
    13021 ctgtaagttt agttataata ttaaaaatag tactttttcc acatccactt ggccctaata
    13081 tagaaacaaa ctcaccttca taaacatcaa tacttatatc ttttaaaact tcaatatctt
    13141 tataagtttt tgatacattt tctatagata cttttacttt tttattgtgg taaaaattca
    13201 tttgtgtaag catcacttgc cttcatatcc ttatctatta atttatattc ttttaagaat
    13261 gatgtatagt tgttccatac actatctttc atttctcccc atcgtggtgc atcatctttg
    13321 tacttgcttg ctaaatattc ttgactcttt aaagctagtt tttcatctac ttcaggtgca
    13381 tgttttacta aaatttttgc tgattcttct ggatttttta ttgcatattc ataacctttt
    13441 gttgtagctt ttaagaattt tttagcaagc tctggattat ttttaagcac agtttcatta
    13501 gaaattataa gtggtgtata ataatccaat ctctcatcta aatcttttac tggtataaag
    13561 tttaagtcat atccaattaa atcagcttta actgcatccc aaccttcaaa tatccatgca
    13621 aaatctacag ttttcatagc tgcaaagaaa tcatcttgac cagtattaac tatctttaac
    13681 ttattaaaat cagctccatt tttcttcata actgctttaa aaacagcttc ttctgatgga
    13741 gagccccaac caccataagt ttttccttca aaatctttag ctgttgttat attcttttct
    13801 tttggtgatg caaacccaga agtattatgt tgtataacag ttgctacagc ttttattgga
    13861 agtggatctt cactagtctt tgcataagta acttgttctt gatagcttat accaaagtca
    13921 gcttttcctg ttgctactaa agtttctgct ccactttcag gtggttgtac tatttcaaca
    13981 tccaaacctt cttctttata gtaaccctta tcaagtgcta caaataatcc agtatgattt
    14041 gtatttggtg tccaatcaag caccatagtt acttttttag aagaagtatc tttatcattg
    14101 tttttgtctt tattgttatt tgaagaacat ccagttattg ttgccaatgt aaaaacactt
    14161 actaaaccta atattcctaa ctttttaaaa ttcataattg cctccctcaa atacctttaa
    14221 tctattaatc tatgtcttat aacaactttt tttacagcct gtacaattcc tacaaacatt
    14281 aaactaaata taactactaa tatagtagat gcaaatactt tatcaagtgc atatgcactt
    14341 tttgacctaa gcatataaac accaagtcct ttcgttccac ctagccattc tgcaacagtt
    14401 gctgccatta cagcatacgt tgcagatatt ttaagtccag aaaaaaattt atccattgcc
    14461 ataggaaatt taaggtgaat gaatgtatta actttatttg agtccatagt cttaaataaa
    14521 tttaaataat ctttatcaat attctcaatg ccatctacaa aacttattaa tataggaaaa
    14581 aaacaagtaa gcattaccat caatactttt ggcattgttc caaatccaaa ccatatcata
    14641 aagagtggtg ctataattat tgtaggtatc atctgtgtaa ccaacataat aggataaaga
    14701 cattttttta ttattgatac aaaatccatc aatattccaa ttaccaatga tattataatt
    14761 gcgactataa aaccaagtat tgcttctcct atagtaacca cagcatgtat ggataaattt
    14821 tgataatctt taaaaaacac attaattata tctactggtg tgggtagtat atactgagga
    14881 acttctaaaa ctcctaccat agattgccaa agaataagta taaacaaaaa tgttgctatt
    14941 ggatatatac tatctttgac tttatctatg atttctaagt ttttcatcta tagtcagacc
    15001 tccagctttt tcatctactc tagctactgt tatgactctt tcacatccaa gattaaaagg
    15061 gattttatgt acctttttta ataattcaaa aacttcatca aaatcacctt caacagtagt
    15121 gctcattgct cctatttcac atttaagacc acttttttca atttcagcaa tacaagcatc
    15181 tactacttta tacatctctt cttcaccttt gtaaggtctt agtggcatta ctgctatgtc
    15241 tgcgattacc atttaaatca ctcctttaaa aataattaat acctactagg tatattttgt
    15301 tttttattaa aaaattttat tactaaaaag atattatatt taaataaaag aaaagtctta
    15361 tccaaaatag gataagactt atttttatct taactggtct ccctacactg gcattatcca
    15421 aacaggttca aagggtcgag ataaaatctc ctctcagcac attagctccc cgaaatatat
    15481 tatatctttt ctacttgatt atactacact tatcatattt taacaaattt tttatttata
    15541 ttagatattt tatccgttca aaaatatcta atataaattg tacattatta caccaaataa
    15601 attatcttaa aatggtaatt tatcaacatc ttccaaaact agattaactg aattttctag
    15661 tttttatttt gctatttcaa gcatatttgg atgtatatca attccataca aatattgtgc
    15721 taaatttctg ttttcactca tctaatttgc aatcgttaca aattacagat tagaaatagc
    15781 ttttagactc tacttatctt tatctacaaa aaatccttca ataaattcat taataatatt
    15841 cagtaatcta aaaataaacc tctatctaga tttttaagat acaagccttt taggaagtat
    15901 cttttgtgat aatataaaaa ttactcatca ttatcatcaa aatcctcagc aaccaacatt
    15961 ttagataaga atcaaactct ataatataat tattttatcc aatttatttt tgagaaataa
    16021 aatatttttt taatttttaa attttttaat atgcaccttg aaaaaagaaa cgttttccta
    16081 tattatggta atagtagaat aaaactaaat aggcaaaact agagaaattt agtgacgcaa
    16141 agctataggg actaagactt atgaaatgag ttatgtcagc cagttgccaa agagatattt
    16201 tctttttgtt tttatgaatg ttttttaggg ggatatatga tatatgttta tggataaaat
    16261 ggatagatgt acacatatac taactgcata tatatgcagt tcacgcgatt actgcaattt
    16321 tatagataca caattaaatg attttatact agaatatgga gagaatgtgg tagaatcttg
    16381 tttgcatcaa gtgatggtat tggtaagcag atataattaa agaaatttta ttttctagaa
    16441 aacccttttt agttttatct acgttttata attcattaaa gaactaatta aaataagctt
    16501 cattatatat ttttcataaa gtaaacagtt gagtttatct catatactgt ttaccccaat
    16561 tacataattc taataatata gattctaatg atttaccacg tttagtagac aaatattcaa
    16621 cttttggagg aatagtcgga taagaaatcc tcttaataag tccatctctt tcaagatact
    16681 ttaattcttg tgacagtatc ttatgactta tatgaacaac ttcttttttt aattgactgt
    16741 aacgtattgg ctcttgtttt aaaatatgcc ataaaataat tgccctccat ttacttccta
    16801 tcacagtcat ggtaaaacca attgaacaat taaaatcttt ttgtctttct tcaatatttg
    16861 gaaattcttc aaaattatta tctatatcca tctcaatcct ctctttcaaa atagtaagta
    16921 tctaactaaa aagtgcattc ttgtttagcg tactaattcg taatataatc ttgattgtag
    16981 tatagagcct tacataatac tactttttta gatgtatatg tttaaacttt aatatagaca
    17041 tatgttattt tattaatttt atttaaacag ataataataa ataataattt atctcaagtt
    17101 ttctatgtag ataaactaat ctaatgttta gaaaggatta tataatatga ataaaaaatt
    17161 tcgtctttat caagttgact cattacaaaa gaaaaattta caggtaatcc agctggtgta
    17221 attacaaatg ctgatggctt atctgaatca caaatgcaaa aaatagcaag agaattaaat
    17281 aattcagaaa ctgcttttat tttcaataac ttggctaaaa atcatcaaga aaattatgat
    17341 gtacatatca gattttttac actaactaat gaggttccta tatgtggtca tgctactatt
    17401 gctgcccatt atgttagagc agtagaaaac caattcaata acaaaacagt atttcaaaaa
    17461 actagtgctg gaatattacc agttgatatt ataaaaaaag ataatgatta tgaaataatt
    17521 atgacacaag gaaaaattga agtatctcca ccgttacaac aaacctatca aaaaagatta
    17581 cttaatgcat ttggtatttt aaaggaagat attcgtacag attgtcctat tgccatatca
    17641 tctacaggac attcaaaagt tatgattgga ataaaggaat tatcaacatt aaatagttta
    17701 tctccaaatt tatatgaatt atcgaaaata agtcaagaaa taaaatgtaa tggatattat
    17761 atttttacat tacataataa agaaactcta caagttcatg gaagaatgtt tgctcctgca
    17821 attggtattt cagaagaccc tgttacagga aatgctaatg gacccttagg tgcgtacctt
    17881 gtacattata aagtaatacc tacacccaac acagagttta tatttgaagc agttcaagga
    17941 gaaacaataa aacgcaaagg caagatgtta gtaaaagtaa ttattttaga taataaacca
    18001 gttgaagtaa aaataattgg aaaagctgta attgcattct caacaaacat agaaatctaa
    18061 ttatagattc cttctaagta aatttttgaa ctttttaata ctaatttaat aacaaactaa
    18121 tttatataca atcaa
//
LOCUS       DAEMGG010000079        17673 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEMGG010000079 DAEMGG010000000
VERSION     DAEMGG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.079"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    436..666
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            774..2255
                     /locus_tag="KPK24_002461"
     CDS             774..2255
                     /locus_tag="KPK24_002461"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG2300128.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGTSSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2265..3158
                     /locus_tag="KPK24_002462"
     CDS             2265..3158
                     /locus_tag="KPK24_002462"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG2300129.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3282..4343
                     /locus_tag="KPK24_002463"
     CDS             3282..4343
                     /locus_tag="KPK24_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG2300130.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4498..5010)
                     /locus_tag="KPK24_002464"
     CDS             complement(4498..5010)
                     /locus_tag="KPK24_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG2300131.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            5530..6465
                     /locus_tag="KPK24_002465"
     CDS             5530..6465
                     /locus_tag="KPK24_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG2300132.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(6557..8428)
                     /locus_tag="KPK24_002466"
     CDS             complement(6557..8428)
                     /locus_tag="KPK24_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG2300133.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYVSLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            8618..9544
                     /locus_tag="KPK24_002467"
     CDS             8618..9544
                     /locus_tag="KPK24_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG2300134.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9683..9829
                     /locus_tag="KPK24_002468"
     CDS             9683..9829
                     /locus_tag="KPK24_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300135.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(9934..10458)
                     /locus_tag="KPK24_002469"
     CDS             complement(9934..10458)
                     /locus_tag="KPK24_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2300136.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(10511..10735)
                     /locus_tag="KPK24_002470"
     CDS             complement(10511..10735)
                     /locus_tag="KPK24_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300137.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            10932..12056
                     /locus_tag="KPK24_002471"
     CDS             10932..12056
                     /locus_tag="KPK24_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBG2300138.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(12530..13354)
                     /locus_tag="KPK24_002472"
     CDS             complement(12530..13354)
                     /locus_tag="KPK24_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300139.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNDNIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(13702..14757)
                     /gene="ispG"
                     /locus_tag="KPK24_002473"
                     /gene_synonym="gcpE"
     CDS             complement(13702..14757)
                     /gene="ispG"
                     /locus_tag="KPK24_002473"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG2300140.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(14940..15944)
                     /gene="rseP"
                     /locus_tag="KPK24_002474"
     CDS             complement(14940..15944)
                     /gene="rseP"
                     /locus_tag="KPK24_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG2300141.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINAS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(15957..17111)
                     /locus_tag="KPK24_002475"
     CDS             complement(15957..17111)
                     /locus_tag="KPK24_002475"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG2300142.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(17275..17478)
                     /locus_tag="KPK24_002476"
     CDS             complement(17275..17478)
                     /locus_tag="KPK24_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300143.1"
                     /translation="MISYAPLWKLLIDKKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         6103 a         2732 c         2076 g         6762 t
ORIGIN      
        1 tttaatttga atatatatgt aatttcacac taacaatttc ttcaaattaa acattattaa
       61 ctatatactt ttgttgaacc aattatgatt aatagtttaa atattagcac tatttactta
      121 tatttatatg atataccata taattacatt tttcaaatta aagttttgta aacttttttt
      181 gtaatatagt gtagtataaa attgtaatat tacagatatt taatagttat cttttgatta
      241 ctttcattaa aaacagcttc atcactatga aaatctaggt ttttaaactt ttaaaaaagt
      301 taaaaaaata tttttctaat actcaatttg attacaattt ttttacaatt taaaaaatat
      361 tttttccaaa tttttatatt aatacttgac tttgtctgtt ttataaacta gaataattaa
      421 taatattaag aatattgaaa ggcattgatt aggaagagta aatctgaatc gattaccaga
      481 gaaggaaact actagctgaa atgtttccct aattagaagg attgaaaact acctaggagc
      541 ttctatccga agttatctta atgattttag taggaataga cgtttttatt acttgcgtta
      601 aagtatcaag tggatttttt aaatcaattt gggtggaacc gcgggaaaca gattctcgtc
      661 ccttttttag ggatgagggt ctttttttat tacctaaatt cagtttttaa aatattctgt
      721 atctaattta attagctaat taaaaattta aaatatatgg aggattataa aaaatgtatt
      781 attcaagtac gagaggcacc gaagaaaaag taactgcatc acaagcaata ataaaaggaa
      841 catcaagtga tggtggtctt tatgttccaa gtgaatttcc caatgtaaaa aatgaactta
      901 taaatttagt aaatttaacg tattctcaga tagcattttt tgtacttagt aaatttttat
      961 gtgattttac tgaagatgaa attaaaaatt gtatagaaaa tgcatatgat gaaaagtttg
     1021 attgtagtag tatagctcca ttaaataaag taaatgatac ttatttctta gaactatatc
     1081 acggtccaac tttagcattt aaagatatgg cacttactat aatgcctcat cttcttaaaa
     1141 catctataaa gaaagataat ttagagaaag atgttgtgat tcttactgct acatctggag
     1201 atacaggtaa agctgcactt gagggattta aagatataga taaaataaag ataattgtat
     1261 tcttcccaga agatggtgta agcccagtac aaaaacttca aatgaaaact caaactggaa
     1321 aaaatacata tgtagttggt ataaaaggaa attttgatga tgcacaatca ggtgtaaaaa
     1381 agattttttc tgataaagaa tttaatcaaa aactattgga taataattat atattatctt
     1441 ctgcaaattc aattaatata ggaagacttc ttccacaagt tgcatactat ttttattcat
     1501 atatgaaatt agttagcaat agtgatataa atttaaatga taaaataaat tttgttgtcc
     1561 caactggtaa ttttggtaat attctagctg gatattatgc caaacaaatg ggtcttccaa
     1621 taaacaaatt aatatgtgca tcaaatgata ataatgttct ttatgatttc tttaaaactg
     1681 gtgtatatga taaaaataga acattgaaat taactacttc tccttctatg gatatattaa
     1741 tttcaagtaa tttagaacga ttactgtttg aaatatcaaa tagaaatact gatattgtaa
     1801 ataagctttt atcagacctt agtgataaag gagtctacaa aataagtgat gatatggaaa
     1861 aaaatttagc ctcattctat ggagaatatt caaatgaaga agaagttaaa agcacgataa
     1921 acaatgtgtt taaaaattat aattatttga tagatactca tactgctgtt gcatacaatt
     1981 gttatgaaaa atataaaaaa gaaacttctg ataacacaaa aactgttatt gtaagtactg
     2041 caagtccatt caagttttct gaagatgttc taaagtctat agattgtgat tttaaagacc
     2101 ttgatgactt tgctataatt gatagattat caagtatttc taaaatagat attccaaaac
     2161 ctattaaaaa tcttaaaaat gcagaaatac tacataaaga tatttatgaa aaagatgaat
     2221 taaaactagc tataaaaaaa ttcttaaagg tataggtgat tattatgtta gagattatag
     2281 ttcctgcaac aagtgctaat ataggtccag gatttgattg tcttggaatt gcgttaaata
     2341 tatacaataa attttatgtt gaagaaattg aaagtggtct tgaaatagaa ggttgtgaag
     2401 atgcttacaa aaacgaaaac aacttagttt atacatctat gaagtacttt tttgatagag
     2461 ttaaaccaga aaaaatacct gctggtatca aaataaaaat ccaaagtgaa gtacctatat
     2521 gtagaggtct tggcagtagt gcctcttgta tagtagctgg agttatagct gcaaatgctc
     2581 tatcaggtgc taatttagat aaaaatcaat tactaaatat agcttcagaa atagaaggtc
     2641 acccagataa tgttgctcct gctattttag gaaatatgat tgtatctgtt actgataatg
     2701 agaacataca ttatgatatt ataaaaattc ctgaagaact taaattttgt gcaatgatac
     2761 ctaattttaa gttatcaaca gaaaaagcta gaggtgtttt accaaaggaa ataccatatt
     2821 ctgatggggt attcaatgtt agtagagttg ctttactaat atctgcctta ctaaacaaaa
     2881 actttgacct acttaaagta gcttgtcaag acaaattgca tcaagattat agaggaactc
     2941 taattgagaa ctacaatgat attgttgaaa aatctgaaca attaaatagt attggagtat
     3001 ttttaagtgg agctggacct actataatgt ctttaataaa agaaaatgat gatagttttg
     3061 tggataatat gaaaaattac ctacaaaaac taaaatctga ttgggaaata aaagagttat
     3121 gttgcgattc taatggagct gttttaaata taatataatt atcgtcttct atacaaaata
     3181 taactactct tttattataa tttgtagtat aataattagg actaggtaat aatattaagt
     3241 attataatct agttctttaa acatgtttac attgaggagg gattatcata aaagagttaa
     3301 taattagaga tttagttgca aaagttccta taattcaagg tggaatgggc gttggtatat
     3361 ctaggtcatc actagctggt gcagttgcta agcttggtgg tgttggtgta atttctggag
     3421 ttcaaattgg atatgatgag gaagattttg aaactaatac tataaatgca aatttaagag
     3481 ctataaaaaa acatatttct aaggccaaag aaatctcaaa tggtggtatt attggtatta
     3541 attttatggt agctatgaaa gaatatgaaa catacgtaaa ggaagctgtt aaagctggtg
     3601 tagaccttat aatatctgga gctggattac ctaataagct accatcatta gtaaagggaa
     3661 gtaatgtaaa aatagcaccc atagtttcaa ctgcaaaagc tgctaatgta attttaaaaa
     3721 tgtgggatag aaaagagaaa actactgctg acttaatagt tgttgaaggt ccaaaagctg
     3781 gcggtcacct tggatactct aacgaggaac ttgataacat agattctata gactatgata
     3841 aggaatttgt tgaaatttta aaggttgcaa atacttatgg agaaaaattt ggtagaaaca
     3901 taccagtagt tgctgctggt ggtataactt ctagtagtga tgttaaaaaa tatatagata
     3961 tgggtgcaag tggtgtacaa gttggtacaa gatttgttgc tacttacgaa tgtgatgccc
     4021 atgagaactt taaaatggca tacataaatg ctagtgaaga agatgttcat atagttaaaa
     4081 gtcctgtagg tcttcctgga cgcgccataa gaaacaaatt tatagaagaa gttaaaataa
     4141 atcgtccaga aataaagaag tgctataatt gtttgattcc ttgtaatcca aaagaaactc
     4201 cttactgtat ttctcaagct ctaatcaatg cagttaaagg agatgttgaa aattcacttt
     4261 tattttgtgg aaatgatgcc tataaaattg ataaattaag tactgttgaa gatgttatca
     4321 atgaattgat ttctgaatta taatttaaag aaaactaaat taagatttta tcaagctaag
     4381 gagtttaaat acgttattta ttaatcttta taaacatatt tatgtcctta gcttttttgt
     4441 gtcaatttcc ttttatggaa attttaattt aattagagat agtattaacc tttataacta
     4501 atcttctttt acaagtacat tggttatttc agctatatat aaagtatgat aatctttttg
     4561 tggataccat cttccatcta taccttcagc aataaaacat tgaggttcaa gtttctgact
     4621 atacattttt ttgcatataa tagccatttt agattcttta aaataaggtg tatcatttaa
     4681 gtgttctata ttaaatccaa tctgtgatat cttatcctct tctctaccag atacttttcc
     4741 acaatatgaa agctctttac gaaaatcttc atcaaaaaat gtaagagaaa atttatctgt
     4801 attatctata aattccttag tataacgctg tggtcttatt actacaaaag caacatcttt
     4861 tccccacata actccaagcc ctccccagct agcagtcatt gtattcactt taccttcatt
     4921 ttctgctgtt ataagtgtcc aatctttacc tatcaatcca aatggattat ttttaatctc
     4981 ttctgccaaa atttctttaa aatcactcat atctctaata acctctcatt ctcttatatt
     5041 tatattcctt ttattttcgt gataaattat aaatgaagtc tagttttgaa aataaaatac
     5101 ttttttaaat atattattgc aaataagtaa atatatatat acactcttat tttttaatat
     5161 atttaataat ttcactcaga atatatatat tctttactca aattaaatat aacttatata
     5221 ctttatgtgt aaattatggt aaaaatatta tttaatcaaa tcaaaacttg atttatatac
     5281 ctggaaaagg tattatatta aagagtgctt aaaatacgca tttcataaat agctatatat
     5341 aaaataataa ataaggagag tgatgggcaa tggatactag tccgttgcgg agagaatttt
     5401 ttacattttc atcacttaat aatatctaaa atcaaaacta atacatatat tcagttatat
     5461 aaattagtta attaatagat actattaaat gatgagattt atataaaact gaataaagga
     5521 tgtgttgcca tgataagata ttacaaaact atagattcaa aattagagaa acttagtttt
     5581 tttgaagatg gctgttggat aaatcttgtt gaacctaatc atagtgaaat caacgaaatt
     5641 tctaatttat taaatataga tgtagaaagc atagaatctg cactagatga agaagaacgc
     5701 tcaagaatag atgttgaaga caatcatact cttatactta tagatatacc agtagacgaa
     5761 agtgactcta attcttctca ttatactaca atacccttag gtattatact aactgaagaa
     5821 gctattgtaa cagtttgtga tgcacaaact aagattttaa atgactttat agttggtcat
     5881 attaaagatt tttttacgtt taaaaagact cgctttttgc ttcaaattct tcacaaaaat
     5941 gcagcttatt acttgcatta cttaagaaaa ataaataaaa tgacaatcat aatagagcga
     6001 gaaatttaca aatcaatgaa aaacaaagaa ttggttcaat tattggaatt agaaaaatct
     6061 ttggtatact tctctacctc actaaaatct aatgaacttg ttttaaacaa aatggttaga
     6121 actgctggta taaagaagta tccagatgat gaagacctat tagaagatgt tatagttgaa
     6181 aatagacagg ctcttgatat ggctaaaata tatggagata tattgagtag aattatggat
     6241 gctttcagtg caattataag taataatcag aataatgtta tgcaaatttt aacagtagtt
     6301 acactaattt tctcaatacc tacaataata tcaggattct ttggtatgaa tgtcatcaac
     6361 atgccatttt ctaataaccc aaacggattt tggataatac ttcttattac ctctataata
     6421 tgtatagtga ttacattttt catgtcaaga aataagctat tataaaatat taataaaaaa
     6481 gccattagaa ttaattgatt tacatttatc ctaatggctt ttatatattt aaatctatat
     6541 aatcttataa tactttttaa ctatcatgtt cttcatctat aatcatttca gtttcaagcc
     6601 cttctagcat tgaatctaaa ctttctttta tatttagtat ataaattggt acatactctt
     6661 cttcatctat tacctcttct tgcaacttaa atggaggaat taatacttca tcaaagtcta
     6721 tatttaaatt aggaaagcct tctactatct cttcacaatt atcgtactct tcatttgcta
     6781 aagaaacgta catatctagg ctctttttat aataaagacc tgcaagcata tactcacact
     6841 tcttacttcc atatttaata ccttctttat aatatttctc agcttctgcg tcatcattta
     6901 attttaaata aatttgacct aaattatata ttgattcaac acaaccttgt gctctagcct
     6961 tttcatacca aaatttagaa tttacataat ctttatagaa catatcataa attaatccta
     7021 ccatatgttg agcatacaca ttattttcat tggcaggtgt atcaaacatc attttagact
     7081 cttccaattt accttctcta aaataaattc tacctagatg tatacgagca ttcatatggt
     7141 ttttgctact tgccatttca aaatactttc tagcattttt tgtatttcct aagctctcat
     7201 atatacaccc caatctataa ctagacctcg tatctccatt ttcagatgct ttcttatacc
     7261 aacttattgc ttcttcaata ttatttaaat cttcatagat atttcctaat ttgatttgac
     7321 aagatacatt atttggaatc ctcgaataat acgaaatcgc tttttctata tcttgattct
     7381 gataatataa atcccctaga ttttcaaggg aacttttgca tccaacagct atagcatcct
     7441 cataatattt aatagcattt tcataatctt tttcttcaaa atatatacct gccagattat
     7501 tttttgcttc tgttatatca tcttcagcag ccattttata ataagttttt gccttctcta
     7561 tctcattttc tctattatat aaattagcaa gtctatactt agaaagtgta tgattttttt
     7621 ctattgcttt ctcataataa taaattgcat ctcccagctc ttcttttcta tcatatacat
     7681 atgcaagcct atactcagac ttcacatcat ctctatcagc actcttttta taatacttct
     7741 ctgcatcttc taaattatca tttatttcca taatcatacc caagttaaag caacctttca
     7801 tatgacctga cttgtacaac tgtttatata aacttactgt tgatttatta tctttctttt
     7861 ttccatttaa tattgctaaa ttatattttg catcatcatc taaaagcaca gaaccttttt
     7921 tatagtattc atatgcttca tcatctttct ccaaaaaagt atataatacc cctaaattaa
     7981 aacaagcttt acctaaattc ttgtctgctg ctgtttttaa ccatttttca caccttacaa
     8041 aatctttcat tttaaaatgg catacaccta aattattttg tgcttgtaaa taacctaaat
     8101 ttgctatctt ttcataccaa tatatagcat attcatagcc atctaactta tagtacatat
     8161 tacctaaatc aaaagcagca tcttcacttc cacttttata tgccattgaa taccactttt
     8221 ctgcttcttt aaattgtttt ttactttcat atatatttcc taaagtatat tgagatttta
     8281 cgtaattatc caatgcagca ctcttataca actctattgc gctttccatc ttacccatct
     8341 tttcaaggag ggcaccctta gtatgtacga ctttcgggtc attagatata ttttttattt
     8401 tttttataga aaattttgta tttttcatta ttcgctacct acccatttta taaaatatat
     8461 tctctcaaac attattatac attataatct acaattaatg ctataatatt gaatttttac
     8521 cttttgtcac tatattgtaa cattaaattt taaattttat aatcattaga ttttattatg
     8581 taattggtat ataatgtata tatagggggg acattttatg aatttcagca atatttttat
     8641 ccaagttgca gttttattta taattatttt agttggatat tttgtcagaa aattcaactt
     8701 attagatgac cactgcacat ctaagctttc aactttgaca atgacagttt ttttaccatc
     8761 aatgataatt agctccatgc aaattaactt tgatagtaaa atgatacaaa aaatactatt
     8821 gttattattt acatctttga ttatgtatat agtttcaatt ataattgcat ttttattgaa
     8881 atacatttta aaatgtaatg ataaaaaaga tttgggaata taccagtata tagttgtttt
     8941 ttcaaatgtt gcttttatgg gttatcctgt aattgaagca gttttaggtc atgaagcaat
     9001 attctatact gcaatattca atttgccttt taacttattt tctttcactt taggaattta
     9061 tcttttatct aaaggcagta caagtagagg tttttcaata aaatcactta taagccctgc
     9121 tacaatagct attgttattg gacttttttt atttgtaaca ggacttaggt taccccaatt
     9181 tataaacgag ccacttgaga tgttgggaag tataactaca ccaatatcca tgattatcat
     9241 tggaagctta cttgctaatt cttcagctct cgattgtttt gtcaacaaaa aattatatat
     9301 agtcactttt ataagattat tggtacttcc tgtgattgtg tattttattc taaaaggatg
     9361 gataaatgat aagatgattt tagctatacc agtagtaatt tcttcaatgc ctgctgctgc
     9421 aaatactgct ataatggcta atcaatatga ctccaatata acactagctt cacaatgtgt
     9481 gttttttaca acactatttt cagttatatc tatacctttt ataagtattt tactcttatc
     9541 ttgatttatt tttttatgta ttatataata acatattcaa taaattacaa atataaacat
     9601 tttattgtag tatattgatg gaattttttc ctagatattt gtcaataata tctatatgta
     9661 ttatagaata ggaggcttaa atatggatat ggatttactc tttaaaatag tatttgctat
     9721 agttgctata ggtgtttctg taaaaatcat taaaactgtt gctagtttag cttttaaaat
     9781 agcgttgata ttgctaataa ttttatttgt atatagaatg tttttgtaaa catttaaaaa
     9841 taagtttttt aacacaatag agaagtaaaa tagattatct ttaatgtaaa ttttttatta
     9901 aagacactct attttacttc tctattagct tatttaaatt aaatttttat tatatcttcc
     9961 cataatataa acatcttcaa attttccatc tctgattaca gcatattttt tagttccctc
    10021 tatttcaaaa cctagctttt tatataaatt taatcccttt tcattatcca caattacttc
    10081 taatcctact ctagttaaca taatccaatt atctgctata tctaaaagtt gttccataag
    10141 cttcttacct actcctcttc cctgataatt ctctgcaaca cttattccta tttctgcgct
    10201 atgtctcaat cgtggagaag aatttacatg taacccagca aaacctatta catctttttc
    10261 tccattatta ttagtttcag ctacaaacat ataatcattt tctcccaaag aagctaacat
    10321 agcttcagtt ttatcgactc tgtcactcat taatgccaaa gtatttctca taacactgta
    10381 tgttcttcta atttcattaa aagcttgtgc atcttctctt cttataggtc ttattgttat
    10441 atctataggc ttactcatat tggattcccc ctcaaacata atctttattt ttaattaaaa
    10501 ttttacatct ttattttata ctgttcaaat ctatgtatgc ttctacctga ggtacatata
    10561 tcatattatc ttttatatgt gtacttccaa attcttttaa ttctgtacct tctttatcct
    10621 tagttatttt atatctaatg gattccatac cttcaactac ttcagcatga aaaaacatac
    10681 cttcatattg tttaaatcta aaattaaaat tgtctttagt aagttttatt atcatatttt
    10741 catctactcc ttatataatt gatatttttc ttattattag ttgtatatat actgcttatt
    10801 catattaata tattataact tatcatttat ttagtataaa gttataaata gatatattta
    10861 tacttatttt acaatctttc ttcaattgat atataatatg tttatataaa ctaatgtatg
    10921 tgaggtgaaa tatgagcaaa aaaacaaaat atttttcttt aattctcttt cctattgctt
    10981 tgcttttaaa ttttatagcc agtaaagttc caaatattgt tgaaaaatat tactcccaat
    11041 tcatagataa aataattgta caaatattga gtaaagtttc tggaattttc ccattttctc
    11101 tctatgagat tacaatgtac ataatagtta tatctatctt cttattttta tgttgtactt
    11161 tatctacaat ttttaataag aaaaaaaaat taaacatata tttaaaaaat tccattttaa
    11221 atatattatc cattgtatca atagtctact ttctttttgt agtgctttgg ggaataaact
    11281 ataataggat tccactagaa accacactta taaataacta taatttaaaa aacaatacct
    11341 ctatccaatc aaaacaatat agtgttaaag agcttactaa attatataaa ttccttgtaa
    11401 ttaaagcaaa tgaaactaga aaactcacat tacaagataa aaatggtatt gtaaaatcta
    11461 atacagatta taatggtgtc ataaatagag ctcaactagg atacgataat atcttggata
    11521 tattacctag tgttagtggt agttattcta agcctaagta tattatttca tcaaatctaa
    11581 tgtgctatac aggaataact ggtatatact ttccttttac aggtgaggca aatgtaaata
    11641 tagcaatacc tgacttatat attccttgta cagttgaaca tgaaatggca catcaaaggg
    11701 gatttgctag tgaagatgag gctaatttta tagcgtatct tacttctata aaacacccca
    11761 atattgattt taactattct ggatatattt tagctttaaa ttatactgct tctgctttaa
    11821 gtaaggttga ttataatgca tatgttgata tttctgctgg catttcagat tctgtccgta
    11881 gagatttaaa aaatgaaagt gaattttggc aaaaatatga aggtaaaatt aatgaaattt
    11941 ctaatgagtt taacaattct tacttaaaag ctaatggcgt tactgaaggt actcagagtt
    12001 atggtaaaat ggttgattta ttgctaactt attatgaatt atatccatat aactaaacac
    12061 tacaattcaa aattttattc atatacagct gatattaaat cagctgtttt ctatttaaat
    12121 aaattattta acttcaagca atcttatata atttgatagt acttatacta tctgtataac
    12181 atatcaattc taactatttt agttaaaaca tttctattat cttttttaat aaatgtttta
    12241 actaaaattt ttaaaacata aaacttccac attatttcac tatctatata aaaatctaaa
    12301 atttcacata taaaattaca taactaaaaa atcataaatt tatagaaaac atatattcaa
    12361 aataaaaaag catgtatcaa aacttgacac atgcttataa tcttatgtat atatatcatt
    12421 ttgtcacaat aaaaatttcc ttgtaacact gatattttta aacaaaatag tccttattta
    12481 attaaattaa cattaattaa attcgtactc attttgctat cattaaacat tacttatagt
    12541 aattttctaa tacactattt gaaacttcct tcccttgaaa acttttcaac caattctcat
    12601 atgctgagtt tttccataca ctaccatcat taaatgttac ttgttttaca caagatataa
    12661 tgtactttac accatgctct ccagttgatt gtttccattt atcaaacaat agaaaggcat
    12721 cattttgatt cttacctggt gctattgcat tttgctttgc atattctttc tccatttgtt
    12781 tccattcttt caaccaattt tctacccatg ccttgccttg ttgtttttca aacatactta
    12841 ttatatcctg tggtggtgat tgttgcattt ttctataagt atgtgaatat gattttggag
    12901 acacaggaga tatgcctgta acaataccat agtctacacc agctgggcta aatccaacac
    12961 ttcctacttc gccatctgct gccacatttt gagcatccca atatagctca agtggttttc
    13021 catttttatc atatgctaag catgtaattt cataatccat aattgccttt tcactgctat
    13081 ttgttaagct tataacattg ctaggccaat gatatggagt atcatcatac tgtattttgc
    13141 tattatttat aaataatgga atattatcat tataaacaag acctttacta ctttgaatat
    13201 ctgatacctt ctttgtgaca gtacttaccg tatttgctaa gtatacattt gttttttctg
    13261 aattggtcgt actaagagaa aatgttgttg tcgttccagt aatcagcaaa gcaacaacta
    13321 aaataggtaa gctagatatt tttttatatc tcacagttct tcacctcttt ttctattaac
    13381 ttataataaa acaatttacc aaaacattaa aatctatgtt aacataattt accaactgtt
    13441 ctagtaacta tatcctatca tagttactat caagtttcaa tactattgat ggaaattttc
    13501 actatattaa atcttttttt aattttataa atttacaata tgatttaact catacatata
    13561 tagtcaaatt agaatataag tttcttacat ttaatgaagt ttataaaata aaaaatgact
    13621 agataattaa atactgtaaa taaaaactta ttacaaaagt atttaaaaat ctagtcaatt
    13681 aatatatata tgaattgtat actataattt ttcaatctct tctagcaact catcaactaa
    13741 tttgttacca tcaatcattc taactatttc acccttttta aataaaagac cttcaccttt
    13801 acccccagca atacctatat ctgcctctct ggcttcacca ggtccattta cagcacagcc
    13861 cataattgca acagtgatat ttttttccat actaccaatc ttttcttcaa cctcatttac
    13921 aacactaatt aagtctatat tacatctacc acatgtaggg catgatataa ctttaatctt
    13981 atcatttaaa agtcccaaac ttcttaaaat ctgtttacca acaataacct cttcaacagg
    14041 gtcgcctgtt aaagatattc taacagtatc tccaattcct ttaagaagaa gtgctcctac
    14101 ccctatagaa gactttattg ttcctttgtg aacacttcca gattctgtaa ttccaatatg
    14161 aagaggataa tcaacttttt ttgatattaa ttcatatgca tcaacagttt tataaatatc
    14221 ggatgatttt agagatataa ctatattatg aaaatctaag tcttcaagaa tcttaatatg
    14281 tcccattgca ctttcaacta atgcttcagc agttgcagag ccatactttt ttaaaagttc
    14341 tttctctaaa gaacctccat taactccaat tctaattttt aaatttcttt ccttgcattt
    14401 ctcaactacc atcttaactc tctctatact tccaatatta ccaggattta ttcttattcc
    14461 atccacacct tgttctatag cttcaagggc tagcttataa tcgaaatgta tatctgcaat
    14521 aattggtata tttacactag actttatctt tgctatattt ttagcagctt taatatcagg
    14581 aacagcaact ctaactatat cacaaccagc ttcctctaat ctctttattt gagctattgt
    14641 agcatctgca tctcttgtat cagtatttgt catagattgt atacttatag gattttctcc
    14701 tcctattttt acagttccta cacttacttc tctcgtcttt cttctctcgt aactcatatt
    14761 ccacctctat atattatttt atattgaatc tctttaaata ttaaaatatt ataacctaca
    14821 tgtatattat aaattaaaaa gctatctagt taaataagaa taaatttcat atataaaaat
    14881 taatttattc ttatttaaaa ctacatagcc tattttctta aaatctctaa aaaactaaat
    14941 cattgaaata atcttagtat atctttatat gtgataaaaa gcataaataa cattaaagct
    15001 ccaaaaccaa caacatttat catgccttct ttatttgggt cgagtttttt accacctcta
    15061 acagcttcta gtaacaacat aagtattctc catccatcaa gtgctggtat tggtaataga
    15121 tttactatac ctaaatttaa gcttatgaca gctgctaaat acactacatt tatgatgcct
    15181 gtacgagcag catctgatac cataccaata acacctactg gtcctgctac agcattgcca
    15241 atccctcctg gtacacgtcc tgtaatcatt tgacctagaa atgttagcat ctgttttgtc
    15301 atatctacag tagttttaca tgcatttact attgacccta aaaagtcttt ttctctttgt
    15361 ggttgaatcc ctatttcata ctttccattg ttatttttag gtgttacatt tacaacttta
    15421 tcacttccat ctctagttat actaagtttt aattctccac cagaagaagc gtttattata
    15481 tttgcaactt catcccaagt cttaacagaa ttaccattaa ttttatttat cttatctcct
    15541 acttgaagcc ctacagcctg tgcaggagta tctggcatta tctttcctaa tttagtcgtt
    15601 ggagaaccta tatacaaaaa tactggaact aaaagtattg ctgctaatat tatattgaat
    15661 attggtcctg ctacaatagt actaaatctt tgtaatatag acttgtttcc aaaagaattt
    15721 ggacttattt gttcttcatc ttcaccttcc atgctgacat atccaccaat tggaagtgct
    15781 cttatagaat actctgtatc tttttttacg ctatatatct taggccccat acctatagaa
    15841 aactcattta ctttgatacc acttcttttt gcaaatataa aatgtcctaa ctcatgtatt
    15901 aatacaatta tactaaatag tattaatgcg gctattatag tcataaattc accttcctat
    15961 ttttttatca gatttgcaac ataagctcta ctccatctat ctgtttctaa gatttcttct
    16021 aatgttggtt cacttatgct actatgaact tctaaagtct tctctatgta atatggtatg
    16081 tcatagaaac caattttatc ctctaaaaat tcacttacta aaacttcatt tgctgaattt
    16141 aaaacagaag aataagttcc tcccattttt aaacactcat atgccaattt taaacatgga
    16201 aaagtttcta aatctggttc ttcaaaagtt aaagtactaa attttgaaaa attcattctt
    16261 tcaaagctac tttccattct atctggatat gtaagagcat actgtattgg tagtctcata
    16321 tctggacatc ctaattgagc aattattgag ctatctgtat actgcaccat tgaatgaatt
    16381 atgctttgtg ggtgaactac tacatctata ttttcttgtt ctactccaaa taaccatcta
    16441 gcttctatca cttcaagtcc tttattcata agcgttgaag aatcaatact tatttttctt
    16501 cccatactcc aatttggatg cttcaaagct tcatttttag ttatatttgc aagttctccc
    16561 tttttctttc ctctaaatgg accaccagat gctgtaagta ttatcttttc tatatttttt
    16621 ttattttctc catttagaca ttgaaatata gcactatgtt cactatcaac aggaagaata
    16681 tttacattgt atttttctgc ttctttcatt acaagttcac cagcagttac caacgtttct
    16741 ttattcgcta atgctatgtc tattcccttc tttatagcac aaagagttgg gactaatcct
    16801 atcataccta ctacagcagt taaaaggaca tccacttcat caagtgatga tattattttt
    16861 aatccttcca ttcctgctaa aacttctatt tctatatttt taggcaacat ttcttttaat
    16921 ttcaatgctt tattttcctc aaaaactgtt acatatttag gtttaaactc tactatctgt
    16981 tctagcaata attcaatatt actgttagct gatattgcaa ctatctcaaa cttatctcta
    17041 ttatctctaa ctacatccaa agtttgttta cctatagagc ctgtagaacc taaaattgat
    17101 atctttttca tataataatc ctttctaata ataaatatac cctgtcaaaa cattataaca
    17161 ctactaaaac aattattttt actattcata gaaatttcat atattattcc acagattaaa
    17221 attaatctat tattttttgt attttgtgga atattataaa atattctagt attattaaat
    17281 gccctttttt atttccacaa catcttctat tttacagtct agtgctaaac atatcttctc
    17341 cattgtatcc atagaaacag atttatcatt tcctagtctg cctaaaacag aaatacttat
    17401 acctgatata tttacaaatt ccattttctt tatttttttg tcaattaaca atttccataa
    17461 tggagcgtat gaaatcatgt tatcacctcg caaaatatat tctatcacat tttatctatt
    17521 ttggaaaatt ttcatctatt tagataaata attatttatc taaatagatt tcaattatag
    17581 tttattaaat ataaatatgt taaaataaaa caaaagcata agttcaaaaa ttaaacttta
    17641 tatatattta tctcattatt ttactaaaac ata
//
LOCUS       DAEMGG010000080        17621 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEMGG010000080 DAEMGG010000000
VERSION     DAEMGG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.080"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..735)
                     /locus_tag="KPK24_002477"
     CDS             complement(<1..735)
                     /locus_tag="KPK24_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG2300144.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDI"
     gene            1009..1683
                     /gene="fsa"
                     /locus_tag="KPK24_002478"
     CDS             1009..1683
                     /gene="fsa"
                     /locus_tag="KPK24_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG2300145.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1805..3199)
                     /locus_tag="KPK24_002479"
     CDS             complement(1805..3199)
                     /locus_tag="KPK24_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG2300146.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFTFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3277..4623)
                     /locus_tag="KPK24_002480"
     CDS             complement(3277..4623)
                     /locus_tag="KPK24_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2300147.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      4873..4975
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5069..6445
                     /locus_tag="KPK24_002481"
     CDS             5069..6445
                     /locus_tag="KPK24_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG2300148.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(6559..7704)
                     /locus_tag="KPK24_002482"
     CDS             complement(6559..7704)
                     /locus_tag="KPK24_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2300149.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            7957..8664
                     /locus_tag="KPK24_002483"
     CDS             7957..8664
                     /locus_tag="KPK24_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300150.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            8711..10306
                     /locus_tag="KPK24_002484"
     CDS             8711..10306
                     /locus_tag="KPK24_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300151.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIGSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
     gene            10409..11491
                     /locus_tag="KPK24_002485"
     CDS             10409..11491
                     /locus_tag="KPK24_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG2300152.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            11640..11801
                     /locus_tag="KPK24_002486"
     CDS             11640..11801
                     /locus_tag="KPK24_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG2300153.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            12142..13410
                     /locus_tag="KPK24_002487"
     CDS             12142..13410
                     /locus_tag="KPK24_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG2300154.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            13605..14393
                     /locus_tag="KPK24_002488"
     CDS             13605..14393
                     /locus_tag="KPK24_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG2300155.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            14393..14842
                     /locus_tag="KPK24_002489"
     CDS             14393..14842
                     /locus_tag="KPK24_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG2300156.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            14846..17110
                     /locus_tag="KPK24_002490"
     CDS             14846..17110
                     /locus_tag="KPK24_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2300157.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLIYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGRMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            17250..17561
                     /locus_tag="KPK24_002491"
     CDS             17250..17561
                     /locus_tag="KPK24_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300158.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         6557 a         2067 c         2815 g         6182 t
ORIGIN      
        1 aatatctact atatcaggag taggcgtttt ttctgtaatt atagaactaa tatcttctaa
       61 atgagcaaac ttgaaatttc catcaaattt aaacttttta tcttccataa ctataaatac
      121 ttttttactt gaatttatta tagccttctt cgtaagacca tcttcaattt caaaagttgt
      181 aagtgaagag ttgaatacat ctactccaca acttcccata aaagtcttat caaacttata
      241 tttcgatata aactctatag ttggagtacc gacaaaaccg tctaaactga gatttaataa
      301 acctccagtg gaaattatat ttaaattatt tggttttgaa gtaactacat ttagtatatc
      361 cagcatattt gtaacaagtg ttactttttt attactttca gctaacaact ttgcaagcaa
      421 gatgttaatt gtagagatat ctaaaaatat agtttctcta tcttcaatta aattaaatgc
      481 tttttctgct attattttct ttgttggaat attaatttct tttctgtctt ttgtatcctg
      541 attttctagt gtttctctag ctagtacagc tcctccataa attcttctta ttgaagaaat
      601 cttttctata ttttttaaat cttttcttat acaatcttct gtaacattaa atttagaact
      661 gagctctttg acagtaactc taccttcttt ttttaggata tttaatatag cttggattct
      721 ttcttctgca aacattttct acctcttttc gcaatatagt attatagtaa atattataag
      781 ataaaataca atatatcaac tattttaaca tgaaatacaa aattaattta atataaaaag
      841 tatgtttttt ctaactatat atttagaatt tagtaaataa aaaaattata aaactaagaa
      901 ggaaaagtaa aacaataata aaaaataaaa aacaataagc aaaatgttga aaataatacc
      961 aaatcaatat ataataagaa taaagaaaga ttaaaggggg atattttaat gcaactaatc
     1021 ttagatacag gtaatgtgga ggagattaag gagttatgta cttgtttacc aatagatggt
     1081 gtaactacaa atccaagtat tgtttcaaaa gaaaaaaaga attttaagca acttataaat
     1141 gaaattggtg agattatagg tgaggatatg cctatacatg ctcaagtgtt aagtacaaag
     1201 tatgaagaaa tattagaaga agcattatat ataagtagtt tgagaaaaaa tatatatgta
     1261 aaaatacctg taacacaaga tggtttaaga gctataaaag accttcacag aaaaggtgta
     1321 aaaataactg ctactgcaat attcactgct catcaaggtt ttttagctgc aaaggctgga
     1381 gcaaattata ttgcacctta tgtaaatagg cttgataata tatctggtga tggagtagct
     1441 atggtttcag aacttataaa aataatagat acttataaaa tggatacaaa agtacttgca
     1501 gccagtttta aaaatgcaca acaagttata gaattaatgc aacatggggt gcatagtgtt
     1561 acagtaccat atgatatttg taaatctatg atgaatcatc cattaactga ttggagtgta
     1621 gataagttta tagaggattg ggagaatact tttggaaaag gaagtaaaac aaataatatt
     1681 tagtattatg gtaaaagagt agttttgtcc aatttcacaa gaacttatat aaaaagatga
     1741 aaatatgaat tttcttttta ataatttcgt tattatctta agttaattca tattttcatc
     1801 ttttttagat ttctagtcct ataaatttcc accaagggaa tgttatacat attattataa
     1861 agtaggttaa aggaatcaaa gttaaaccgt atttgaaaat aaccttgcta ttatacagat
     1921 tttcaccatg accaaccatt attgtagcat gatgaaatgg taatatgtac tgtatgctta
     1981 ctataatgta gacaaataga cataaagtaa cactattttc ctttagtata tgtaaacttc
     2041 caagtaatgg tattactaca gatgatgtag tgacagcact tccaagacat atattcaaga
     2101 ttattgtgag agttatcaaa aacaatattg aaataatttc tgaagagttt ggtataaact
     2161 ttatcaaata atttcccatt atatctacaa ctccagagta ctttaacact cctccaattg
     2221 aaaatgctgc tataaaaaaa ataaggacct tccaatttat ttcttttaaa gtactaaatc
     2281 caactatttt tcttagtaat aaaagaagta cacaaattga cattataatt aatgtgttta
     2341 tgttatgtat gaactgagtt aaaaacataa aaaacattat tcctaggcat aaaaatacgt
     2401 gtttttgttc tttgtttagc aaattaaatt ctttttttaa attagaatat gaaaaatgaa
     2461 gtttattacc ctcaagattt tcattagaaa taaattttat tgaatgcaat tcttttttaa
     2521 agacaaaggt aaataaaaaa taagttataa aacaagtaat tatagttgga acagacatat
     2581 aaaaagccca ttgaccccaa tttatattac aatttccaag ttctagtaca acataattta
     2641 gaagagtgtc tccatttaca aaaaacattg atgtaaaggt agatgctgta aatatactaa
     2701 ataaaagtat agattttgaa tcttcagtta tctgttgttc ttttaaaaac tccttgtaaa
     2761 aagcagatac aagaataacc cttggaaatg gttgtgggat aaaaaatatt aaaagtatac
     2821 ctaaaatata cgataaaaga atcatttttt taggggtatt tacagtattt aaaattaact
     2881 tctcagaaat aatttttgcg acacctgttt ttgtaactgc attagtaatt atgtatgcaa
     2941 gaattattat gtaaaaattt tctgtaaata aaaactgtaa aataaccttt attggtgcaa
     3001 tgttaaatat aaacgctaga attacaaaaa acaatgcaac aaaagttttt tcaactgagt
     3061 ctacagccca tatcaagata gttaaaaaag ataagcatat agaaaatttt atgttgaaac
     3121 tgtaattaag agagaagtag agaattatgg tagctataac agtcatataa aattgttttg
     3181 gcttaagaat aatacccatt tttatattca cctccataat ttattatatc ataatggagg
     3241 tgaatacaat gaatgtgaat acattttata aaaagattat tcattgattc tgtgatttac
     3301 aatagcttta gttttccaat gtcctttctt atagtagata actgtaacaa taagacctaa
     3361 agtccaacca aaaggaatac ccatccaaat tccgtctgtt ccaaaaaatt tagaaagcat
     3421 ataagaagct ggaagtcttc caagccataa agatactatt gagcttataa gtgcaaacat
     3481 ggaatcacca gcacctttca ttgcacttga aagtacaaaa gaagttccaa taaataagaa
     3541 gaatggtcct attataaata agtaagaaga gccaatttta atgacatctt tatcagtatt
     3601 gaatagagca attagatttt ccctaaatag caaaattaaa gcaataactg taactgctaa
     3661 aactaaggtc atttttatag attctcgaat gcctttttta actctttcat tttctccagc
     3721 acctatgttt tgagcaacaa aagtagaaac agcagaacca agattcataa taggtaagct
     3781 tataaaattt tctatcctaa gaccagcacc aaaagctgcc atagcagatg taccataact
     3841 attaactaaa aattgaagag ccatatttcc aattgaaaac aacatttgtt gaactccact
     3901 tggaagtcct aatctaagtc catctttaaa tattttgtta tcatatacaa ttcccttgat
     3961 tttaaattta agcacttcat gttttttatt taaataaatt atacttatta taaatgatac
     4021 tgcctgagcc attatagtgg caagtgcaac gccctcaaca cccattctta gcactacaat
     4081 aaaagttaag tctaaaacta tatttagaat tgtggctatt atcagaaaat aaagtggagt
     4141 tactgaatct ccaagtcctc taagtattgc acttactgaa ttatatccag cagaaaataa
     4201 tattccacta aatattatag taagatacaa ttttgattga gcaaaaacgg attctggtgt
     4261 attcataagc ttaagcattg ggccaccaaa tactattcca agaatactta ttaatataga
     4321 accaataaat aaaagtatat atgtagtatc aatagtagct tttacctttt ttaaatctcc
     4381 tgagccataa aattgtgaca ctaaaataga aaatccaagg ctaacaccca taagcgctgc
     4441 caccaataaa aacattatag ggttagctcc agagactgcc gccattgctt cttttccaat
     4501 aaatctacca actattatac tgtcagctgt attgtatagt tgttgaaata aactaccaat
     4561 aagcataggc atggcaaaaa agaatatgga ctttccctca tgacctgtag ttaaatcttt
     4621 caccttattc ctccaaataa aaaaattatt aatttattat agtataatct taacataaac
     4681 tttatatcca aactataaat aaattgtatc aaaatatcat atattttttt aaaatgtaaa
     4741 gaatataatt ataatttgaa tttaataaat caatatttaa tataatcgaa tattaataca
     4801 aaatcaatac aatatatacg tatatattga caaaattaat tatataaatt aaaataatat
     4861 tcgtaaagaa aaattcatgt tttgtactgt ataacatata attttgacaa tatgggtcat
     4921 aagtttctac cggaataccg taaatattct gactatgtat actattattt agttaattaa
     4981 atatttaatt aactgaatta tgaaggatac ggctcagctt atcaactact gtgtcgtttt
     5041 tttgtaaaat ttaataggag gtgttgttat gcaaacaaca aacagtagta ctatgcagaa
     5101 actttttcca attttgacta ataaaaatgt cgacatgaag aaggaaataa ttgcaggagt
     5161 aactactttt ttaactatgg cttatataat agctgtaaat ccaaatattt tatctgagac
     5221 aggcatgcct gcaggtgcac ttgttacagg tacttgttta gcagcagcat ttggatgtat
     5281 tttaatgggt gtagttgcta accttccttt tgctttagca tcaggtatgg gtcttaatgc
     5341 attttttgca tatacagtag ttttacaaat gggtgttcct tgggaagtag ctcttacagc
     5401 agtatttgta gaaggtatta tttttatagt attatcagta agtggagttc gtgaggctgt
     5461 tgtaaatgcg ataccaaaaa atatgaaatt agctgttaca ggtggtatag gaatatttat
     5521 tgcacttata ggtctagtta acagtggaat tgttattgga gaccaagcca ctcttatcaa
     5581 aatgggtaga tttacaccag cagtaataat aacttgtgta ggattaataa ttattgctgt
     5641 attagataaa aaaagagtta aaggttctat actttttgga atagtagtga gttcattact
     5701 tgcttgggca tttgctttta taaatccaga gtatgcacaa aaattaggta tatatttacc
     5761 tggtggatta tttaaattcg aaagtttagc accaatagca ggtaaaatag atttaggata
     5821 tgtactacat ccaacaaata taggtggttt tttagtagta gtttgtacat ttttatttgt
     5881 tgattttttt gatacagtag gtacattagt tggtgtttgt tctaaagcaa acatgttaga
     5941 tgaaaaaggg aatgttccta atgtaggtag agccttactt acagatgcgg ttgcaactac
     6001 agttggtgca ggacttggag tatctacagt tacaacttat gttgagagtt caacaggagt
     6061 tatcgcaggt ggaagaactg gatggacagc aataacagtt ggaattttat tcttagctgc
     6121 aatgtttttc tctcctgtat ttatagcaat tccatcatgt gctacagcac cagctttaat
     6181 atatgttgga tatttaatgc ttggtacagt taaggatata gagtttgata atataactga
     6241 aggtgttcca gcttttgtta ctattgcttg tatggcatta acatatagta taggtgatgg
     6301 tttaacttta ggtatattaa cttatgtttt cgtaaatata ttttataata tatttggagc
     6361 taaaaaggca gaagacaaaa aacatgtttc tgtagtaatg attgttctgg caatattatt
     6421 tataataaaa ctttggctac tataataggt attatttaaa attttaattt aatataaaat
     6481 aaagggctgt aatttattat caattgtgat gataaactac agctcttttg tatttgttgt
     6541 atttttgtat ttattgtatt atttaataat gtctattagt tctccatttt gtaaagtttt
     6601 aggtgtaatt actatattga actctttttg caaatctttt ataatatcta attctttttt
     6661 tgttattatt gagattgtgt taccacttcg atttcctcta gcagttcgac cacatctatg
     6721 caggtattca tttttacttt ttggaaaatc caagttaaaa acatgactta catccacaat
     6781 atctagtcca cgagcagata aatcagttgt aattaaaatc tttgctttac caagtttaaa
     6841 tttatttatt gcattctttc tatcttcttt ttccatattt ccaaaaattc ctattgcttt
     6901 gtaattatga taatttagtt ttgatactag gacttctata tttttttcat tatttacaaa
     6961 tacaatagct ttttttggat ttgtagctgc taatgccttt ctaagaaaag taaatttatc
     7021 ccttatctca cctaataaat atgagtgatt gatgttagga tttatttgtg atttttcctt
     7081 tgtttttatt atttcaaact ccttcataag tttatcacaa atttttatag tgctatcagt
     7141 aagacttgca gagcatccta taatttgtct atctcttaac gttgttctta ttatgtcttc
     7201 tatacaagtt atattttttc cattcaatag attgtccact tcatctaaga caatagtttt
     7261 tatattatgt gattttagtt tcttttgttt tatcaagtct aaaactcttc cacaagagcc
     7321 aattactatg tgtggtttta ctgcttttat attttttatt tgtttttgta tattgacctc
     7381 cccaattaaa gctaaagaag taacggagag ttctgcattt ttagccaata attcaacctg
     7441 gtttgtaatc tgcattacta attcatgcgt aggtgcaagt ataagagctt gtgtttctct
     7501 ttttgatgta tcaatttttt caaatatagg taaaaggtac gctagtgttt ttcctgttcc
     7561 agtctgtgaa ttaatcaata aatccttatt ttgaattata tttcctataa caagtgactg
     7621 aacttctgtt ggagaagtta tgtcttgttt tttgagtcca tctattagag tggaacttat
     7681 ttttagttgt tcaaatgtat tcatattttg ctccttatat tttaataatt atagtcaact
     7741 attttttatt atacattgtt tattcaaatc gaactatata ttcttttata attatgtgta
     7801 atttgaaagt aaatatcaat tatatttata cctttatatt tctgatacct ttgcatttta
     7861 tagatttcta aaataaacaa ataaaagttg taattgtttg atatttgtta tatattatat
     7921 ataacaaata tcaaaactat aatatggagg gaaattatgt taaaagtaat caatgtatct
     7981 aaatcatata aaaataaaaa agtagtaagt agtgtctcat ttgatgtaaa tgatggagag
     8041 ctttttgggt ttataggaca taatggagca ggtaaaacta ctactattaa agcaattgtt
     8101 ggaatacacg attttgaaga gggagaggta ctaataaatt ctaaatctat aaaaaaacag
     8161 cctgttgaat gtaaaaagga aatggcatat atacctgata atccagattt atacgagtca
     8221 ttaactggta tacaatacct taattttata gctgacatat ttgaagttgc caaagataaa
     8281 agagaagagt taataaaata ttattcaaat aagtttgaac tcaataaagc attaggagat
     8341 ttaatatctt catattcgca tggtatgaaa caaaaattag ctataatttc ggctttgata
     8401 cacagcccaa aaatcttaat tcttgatgaa ccttttgtag gtcttgaccc aaaagcttcc
     8461 tttatattga aggaaattat gagggagttt tgtgacaaag gtggttgtat atttttctca
     8521 acacacgttt tagaagttgc tgaaaaaatt tgtgataaga ttgccataat aaaagatgga
     8581 aatataattg cttatggaac tacagaagaa gttaagggaa gtaattcact tgaaagtatt
     8641 ttcatggaat taatagagaa ataaaatgtt ttttatacaa tatagtgtct aaagtgagga
     8701 atgataaaat atgagcaatc taagtatatt aataaaaaat aatataataa atgaatttaa
     8761 attaaatgcg ttaaaaaaag ctgatggtag agaaaaacaa aaaataatgg gaatgatgct
     8821 ttcagttgtt ataatagcgg gtctattaat tttttatgta acgagcttaa gtctctcttt
     8881 agttgatatc ttaaagcaaa taaatcaact ggaaatgcta ctgatatttg gttttggatt
     8941 atcaacatta acaactattg ttacatcatt atataaaacg tcatcatatc tatttcaagc
     9001 aaaagattta gaccttttga ctagtttacc tataaaagaa tctactgtac tagcaagtaa
     9061 gatattaatg ttggttggag caaactattt attttcttca atatgtatga ttatacctgc
     9121 agtggtttac tttttaaatt ctgatgtaag tataacttat ttcccttatt tgatattgct
     9181 atttttagct ttaccattat taccgatagt tgcttcatca ataatttttc tatttatagg
     9241 gcagatatca tcaagaataa aatacaaaaa tttagtatta atcataggta gcattatatt
     9301 gatgttgctt tataccctac ttatgtcaag acttaattct atgtcagttg aaatgctaaa
     9361 gaatagtgtg tctatttcag agacaattag aaaagtatat cctcctatat attattttgt
     9421 tgatgctctt aaaacaggaa gtatagtttc atttttagca tttatagcca tctcaataat
     9481 accatttttt atgtttgtaa ctctgtttgc aaaaaaattt aaagatataa atgctaagat
     9541 gagtgaagga tataagtcaa aaaattatga atttaagggt caaaaaactt caagaccttc
     9601 aaaagcttta ttaaaaaaag aatttaagag atatacttct tcgtttatat actttttaaa
     9661 ttcatctgca ggtttgttat ttctaatagt aggtacaatt ttaatattat tatttggagc
     9721 agataaagtg ggtgagttac tgaaattgaa tatagatttt aatcttataa aaatacaatt
     9781 attgggagta ttcttcttta taatagtaat gaattgtaca acatactgtt caatttctct
     9841 tgaaggaaaa aacttatgga tactgaaatc ttctccaata gatgaaagag aaatattctg
     9901 gtcaaaaata ttgatgaatt ttttattgaa tgcaccacta tctattttat gtctaatatt
     9961 agtatctata agattagact ttgaaattca gtttattatt gtatcaatct gtttgattgg
    10021 aagtttttca atgtttgtag cattgatggg attattatca aatttattat acccaaacct
    10081 tgattggaaa aatgaggtgg ctgtagtaaa aagaagtgca agtatgatag ttacaatgtt
    10141 agtatcatta gcttatgtaa ttatacttgg aggtatatat gtattacttg gaatttcgtt
    10201 tttaaatata tatattattt tggcaagtat acttactttg atattagatt ttatgatatg
    10261 gagaattatt tctactaaag gagtaaaaat atttaaaaat ttataagtgt atttatgtca
    10321 aataaagact tatctttttt agtcttgcaa ataataggtt aagtagtata ctatgtatag
    10381 tgatatgaaa aaatttagga gaaaataaat gagaaaatta tccaaaagag aaaaagattt
    10441 tattcaatat ggatttttat tatttttgat atgtattact acttatttag tattaactac
    10501 tttagatata aaactaatac ctaaaattat aaggctagta aataagactt atatatttct
    10561 tggcatattg acaattatag tatatatgat aattgaatct attgtaactc atttgataat
    10621 aaatgcagtt caaaaaacaa aagtaaaatt tattggtatt aaaatggcta caatgggatt
    10681 ttactataac ttagttactc catttgcatc tgggagtcaa ccaatgcaaa tatatgcact
    10741 tacaaaatat gatgtaagcc ttagtaaatc agtggctata gttactaata aaactgtagt
    10801 atttcaaagt acagttactg tattttgtgg aattttaatt ttgctaaata agtcattact
    10861 gatggagcaa gtacattctg tgaaagtctt gataactttt ggaatgatta tgaatgtaag
    10921 tatgttgtta ggtggactac taatagtatt tagtccaaaa caagtaaaaa tgatagcaga
    10981 agttttgatt aattttttat ctaaatttaa aacatttaaa tttttagatt caaaacgtga
    11041 tagaataaat cattatattg atgattataa ttattctata aaaatattta ttaaaaatgt
    11101 aagaatctta gttttaagta tattacttac tgtgttacaa ttaatcgcat atttcagtat
    11161 agtttattgt gtgtataaag cctcaaattt aagaggagtt tcatatgtac atataatgac
    11221 attacaggtc tttttatata tggctatatc accaattcca acaccaggaa atgttggagc
    11281 taatgaggtt acatttttta ctatgttctc caatataatt ccaaaagaat tattggggta
    11341 ctctgtactc ttatatagtg ggtttgtata ctattttata ttaatagttt caggtttatt
    11401 tacatttgga acgcattata cattaaattc ttggaaaaag gaagataaag taaattctaa
    11461 tttagataag gttattgaaa aattaaatta ataaaataat tagggaaatt ctatatataa
    11521 atatggtgtt gaataagcta tattatattg agatatttcc cttttttgat taaaattata
    11581 gaaatttgaa ataataagta ataaacagaa aaagtgatat tgttttgaaa ggagaaacta
    11641 tgaaacaaga taaacaaaat gaaaaacaaa gtgagatttc taagataaaa caaagcaatg
    11701 ataataaact tgaaatagct caagaattac atgtaagtaa aaatataggg aatatgcttg
    11761 gatatagaat agataagaaa aaaatgaata gaccaagtta atgaaaatgt tgaaaatggt
    11821 acattttaaa aaatatatat aatgaaatgt tttattgtta aatttaaact tattttaatg
    11881 gaggaaaaag tcgaaaaata tctaaaaaga tatggatata aaaaatatta atatatatat
    11941 actataaact tgacaaaatc aaccttttaa taactagcaa aaaatagcaa aattatataa
    12001 gcgttattta attgtcaaaa atgaaagcta aattgcatgt cctaaaaaaa atgattgata
    12061 acatttattt gtgtgctata attatatagt aaaacaataa gtatattgca taatgtatgc
    12121 aaatcgggag gagagacgga catggaagca tctatacaca caattgccag caataattta
    12181 ttgataattt ttgctattgt aagtataact ggaataattt gtagtaagct tagtgaaata
    12241 ataaaggttc cagatgtagt gttatattta cttgtaggta ttttaatagg accatcattt
    12301 ttaaaattta tagatattcg tggatttcaa atagaaaatc aactaatact tacatttggt
    12361 tctgcattta ttttatattt aggtggaaaa gaaataagtt taaaagtatt gagaaatgtg
    12421 aagatatctg tattcttatt agctacttta ggagttgtga tatctgcttt tataatgcaa
    12481 caagtaatag gatttacttt tgggataagt gcaatgactg ctttactagc aggaactata
    12541 atagcatcaa cagacccagc cactcttgta cctatattta atcaggtaaa gataaaggat
    12601 agggtaaaac aaactgtaat aagtgaatca gcttttaatg atgcaacagg agctatacta
    12661 acatcagcag tgttggctgt aattttgtca aataaatttt cattaggtga aaatatttat
    12721 gaactgggga caatggttat tgtcggagtt ttagttggtt taattacagg aatactgctt
    12781 ttgaaattag ttaatgataa accttatgga atatttaaag attttgcacc aataatatct
    12841 gttatttcag ttgtagttgc atatgaatta tctacaaaat taggtggaag tggatatatg
    12901 tcttgcttta ttgtaggtat aataactggt aacaagaaaa actttaaaat ttggttaagt
    12961 caaaaatctt atgatgcaga tttttatgtt gctgaaactt taggtactat ttgtcgtatg
    13021 gcaatattta taatattggg tagccaagtt aatttggctc agttaagtaa atattttatg
    13081 ccatctgtaa ttacagtatt agtattaatg tttatagcaa gaccactttg cgttttagtt
    13141 tgtacacttg tagacaggga agcaaaatgg agtaaaagtg aaatattatt tatgatgtgg
    13201 gtaagggaaa caggcgtaat accagcagca ctttgtggta ttatttcagc aatgaaagtt
    13261 cctggatacg aagtgatttc atctgtagta ttcatgacta tactaattac attagtaata
    13321 caaggaagta ctacaaaatt agtagctaaa aaacttggat tattagaaga agaagtgata
    13381 aatataagtg aaaaagtatc aactttttaa agttttgctt taaaatagta cctgttaatg
    13441 gctattttaa tattgtctaa tgatgattat tgaaatagtc atttttttat gtataagtaa
    13501 tttacagaat ttacatatta gacatattta ttatcaaaat ttgttgaagc tatgatataa
    13561 ttagttgtat ttaaaataag aatagcaaat aagggaggaa atttatgttt acggttatgg
    13621 atattgtaca gccagataca gtagaagaag cgtactcaat tttaaataaa agaaaaacta
    13681 accaagtaat tggtggaagt gcttttttaa gaatggggaa aaaaagaata ggaacaggaa
    13741 tagaattatc taaattaaat ttagattata taaaagagta tgaagattac gttgagattg
    13801 gttctatgac tacttttaga acattagaaa caagttccat aataaaaaat aattttggaa
    13861 gaataataga agattcagtt aaaaatataa taggagttca gtttagaaat gtagtaacag
    13921 ttggtgcaac agtattttct aaatatggtt tttctgactt gatagtagca ttactttcat
    13981 tagatacgga agtagaatta tataatattg ggagaattag tcttgaagag tttctaaata
    14041 gagattatga gaaagattta cttattaaga tatacataaa gaagacaaat aagaatgctt
    14101 cttataaatc acttagaaat gcaaagagtg attatccaat tctaaatgta tctgtatcaa
    14161 aacatatgga acaatttaag ttatgtgtag gagcaagacc gcagaaagct acgattgcaa
    14221 aacaagctag tgaatttcta tctaacaatg aaatcaatga aattaatata gataaagcag
    14281 tagaaatagc atcagaagaa ctaacttttg gttctaacat gagagcatct agggagtata
    14341 gaaaagctat gagtaaagta cttttaaaaa gagctataat ggaggttatt taatgttagt
    14401 aaagattaat ataaatggga agaaaaaaag tgttgatata gaaccagaag aatatctggt
    14461 tgatacttta agaaaagttg gtaatttaag tgtgaaaaga ggttgtgaca caggttgttg
    14521 tggtttatgt acagttttaa ttgacaagaa acctacatta tcttgtgcaa cactgacatt
    14581 gagagcttta gataaagaaa taactacaat tgaaggttta gaaaatgaag ttaaagaatt
    14641 tagtgaagtt ttggtaaaag aaggagctga gcaatgtggt ttctgttctc ctggctttat
    14701 acttactgta cttgcaatga aggatgagct tgaaaatcct acagatgatg aaataaagca
    14761 ttatcttaca ggaaatctat gtagatgtac agggtatatg ggacagttaa gagctatcaa
    14821 aacttatctg gaggtaaaat agaacatgaa tatagttgga aaaggaatca agaaaataga
    14881 tggaatgagt atcacaacag gaaagcctgt atatacagat gatttatcag caaaagatgc
    14941 tttggtggta aagatactta gaagtcctca cgcttatgca aaaataaaag atatagatac
    15001 atcaaaggca gaaatggttg atggtgttga atgtgtttta atatataaag atgtaccaaa
    15061 atctcgattt acacttgctg gtcaaacata tccagagcca tctccatatg atagattaat
    15121 tcttgaagat gttgtaagat atgtaggtga cgaagtagct atagtggcag ctatagatga
    15181 aaaaactgca attaaagcaa tgaatcttat caaagtaaag tatgaagtac ttacacctgt
    15241 tttggatttt gaagaggcaa taggaaataa aactatggta catgatgaag atagacattg
    15301 taactttgat ataggtatgc aaagagaaaa aaatatagta tcccatcatg attatacaaa
    15361 aggtgatgta gaaaaagtat taagtaattg tgatgtagtg attgaagaaa cttattatac
    15421 tcaagctcaa gcacatgcaa tgatggagac gtatagggca tacaattatt tagaccatat
    15481 gggaagattg gttgtagtaa gttcaactca aataccattt catgtaaaaa gacatttatc
    15541 tcgtgcacta gaaataccag ctagcaaaat tagggttata aaacctagaa ttggtggcgg
    15601 atttggtggt aaacaaactg catgtgttga gattttttct gcaattgtta ctttaaaaac
    15661 agggaaacca gctaaaataa tttatgatag aaaagaaact tttaattgtt caacaagtag
    15721 acatgcaatg agattaaaag ttagactagg tgctacaaat gatggcataa taaaggcaat
    15781 agatataaaa gcactttctg atacaggagc atatggagag catgcttcta caactattgg
    15841 tttggttgga gaaaaaactt taccaatgta taataaagtt gaagcaacaa gatttattgc
    15901 agatgtggta tatactaata agatgccagc aggagcactt agagggtatg gagcatcaca
    15961 aggatgtttt gctgttgaat caactataaa tatgcttgct aataaattaa aaatggaccc
    16021 aacagattta aggttaaaaa atgtggtaac agaaggtgaa actacatttg catatggtaa
    16081 aactttaaat agtgtcgatt taataaaatg tataaataaa ggcaaagaac ttattggatg
    16141 gaatgaaaaa tatccttgta aacatcttga taatggaaaa attagaagtg tgggtatggc
    16201 aattactatg caaggttctg gtatacaagg tcttgataag tcttcagctg aaataaaatt
    16261 gaatgactgt ggagattata ctcttatgat aggttctact gatatgggta caggaagtga
    16321 tacgatttta gctcaaatgg ctgctgaggt tcttgataca agcattgaaa atataacagt
    16381 tatatcagct gatactgact tagttccata tgacccaggt tcatatgctt caagtactac
    16441 ttatgtaaca ggtatggcag tagttaaggc agctcaagac ttaagagaaa aaataatttc
    16501 tgctggagca actaaactag gtgttgagaa ggatgaagta gaatttgatg gtataaaatt
    16561 aactagtaaa aaaggtagca ctttattatt cgatatagca gttgattcta caattggtcc
    16621 agatactaat caattaatag gtcttgcttc tcatggtagc ccagtatctc ctcctccttt
    16681 tatagcaggt tttgcagaaa ctgaaattga cacagaaact ggaaaagttg atttagtaaa
    16741 ttatgtagct gtagtagatt gtggaacggt tataaataaa aatctagcta agatacaagt
    16801 tgaaggtgga attgttcaag ggattggaat ggcattattt gaagaagtta gatacacaga
    16861 aaaaggaaga atggacacaa atacatttat gcaatacaag attccagcta gatgtgatat
    16921 tggaaatgtt aaagtagatt ttgttgaaac ttatgaacca actggaccat ttggtgctaa
    16981 atctgttgga gaagttgtta taaatacacc tttaccagca atacaagaag ctgtgtttaa
    17041 tggaattggt gtaagaataa atacactccc tattacacct gaaaaagttt ttatggaaat
    17101 gaataaataa ttatttatga aaatttagac tagttttaca tgttaaacaa ccagttatgt
    17161 catacctgtt gaaagatatt taataagtag ttattataat attatgaatt ttacttataa
    17221 aatacaaaat taaataaggg gatgatgttt tggctggtaa tctaaataat atgagagcag
    17281 taaataattt tagaggagat aagaacattt tagaatgttt agtcagcttt gagggtcgtt
    17341 caataagtca gagaaaagta agggtatttt ttaaagaaaa acaaaatcaa atagaaattg
    17401 attttgcaga agaggaaatt tctaaattgg ttgaaaatgt tgttttaaat acatcatatc
    17461 aagaaatgtt atatgatgaa atagagaaac aactggaaat tgattgtata ggtacttgga
    17521 tgatattatc taaattaaaa gatggtagta gagttcactg attttaaaat aaattattag
    17581 agcctaattt taaggctctt ttttgctgtt tttaattaac a
//
LOCUS       DAEMGG010000081        17406 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEMGG010000081 DAEMGG010000000
VERSION     DAEMGG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.081"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            132..1937
                     /gene="lepA"
                     /locus_tag="KPK24_002492"
     CDS             132..1937
                     /gene="lepA"
                     /locus_tag="KPK24_002492"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG2300159.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            2086..3423
                     /locus_tag="KPK24_002493"
     CDS             2086..3423
                     /locus_tag="KPK24_002493"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG2300160.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            3656..4969
                     /locus_tag="KPK24_002494"
     CDS             3656..4969
                     /locus_tag="KPK24_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2300161.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            5141..6316
                     /locus_tag="KPK24_002495"
     CDS             5141..6316
                     /locus_tag="KPK24_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG2300162.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            6419..7438
                     /gene="hrcA"
                     /locus_tag="KPK24_002496"
     CDS             6419..7438
                     /gene="hrcA"
                     /locus_tag="KPK24_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG2300163.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            7468..8088
                     /gene="grpE"
                     /locus_tag="KPK24_002497"
     CDS             7468..8088
                     /gene="grpE"
                     /locus_tag="KPK24_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG2300164.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            8180..10027
                     /gene="dnaK"
                     /locus_tag="KPK24_002498"
     CDS             8180..10027
                     /gene="dnaK"
                     /locus_tag="KPK24_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG2300165.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSESEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            10238..11392
                     /gene="dnaJ"
                     /locus_tag="KPK24_002499"
     CDS             10238..11392
                     /gene="dnaJ"
                     /locus_tag="KPK24_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG2300166.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            12358..13293
                     /locus_tag="KPK24_002500"
     CDS             12358..13293
                     /locus_tag="KPK24_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG2300167.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EGLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            13329..14702
                     /locus_tag="KPK24_002501"
     CDS             13329..14702
                     /locus_tag="KPK24_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2300168.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(14890..15657)
                     /locus_tag="KPK24_002502"
     CDS             complement(14890..15657)
                     /locus_tag="KPK24_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300169.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(15687..16796)
                     /gene="ugpC"
                     /locus_tag="KPK24_002503"
     CDS             complement(15687..16796)
                     /gene="ugpC"
                     /locus_tag="KPK24_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG2300170.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            17230..17406
                     /locus_tag="KPK24_002504"
     CDS             17230..17406
                     /locus_tag="KPK24_002504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300171.1"
                     /translation="MNLKKKEKIVKPVLKKLTEIVLNILSKKILNYAIYAISVLIVTK
                     VVINIDLYISILYY"
BASE COUNT         6776 a         1921 c         3049 g         5660 t
ORIGIN      
        1 acatataatg tggataattt tgtttagtgc caattagttt atattcaaaa attttaaatt
       61 tggtatataa agagttttta gataatgtaa aataataact ataagaataa tcaagaggag
      121 ggaattttaa ggtggacaat aaacaaagta gaactagaaa ttttagtata attgcacata
      181 tagaccatgg aaagtctacc ttagctgata gattaataca acaaactggg cttgttagtg
      241 aaagagacat gaaaagtcaa ttacttgaca atatggacct tgaaagagaa agaggaatca
      301 ctataaagct tcaaaacata agattaatgt ataaagcgaa ggatggaaat gaatattatt
      361 taaatcttat agatacacct ggacatgtag actttaacta tgaggtatca agaagtttag
      421 ctgcatgtga aggagcacta ttagtagttg atgcagctca aggtgttgag gcacaaactt
      481 tagccaatgt ttatcttgct atagaccaag atttagaaat attaccaata ataaataaaa
      541 tagatttacc aagtgcaaga ccagaggaag taaaaaatga aatagaagat ttaatagggc
      601 ttgattcaag tgaagctcct cttatttcag ctaaaactgg tttaaacata gaagatgttt
      661 tagaagatat tgtaaaaaat gttcctccac ctaaaggaga taatgaagct ccattaaagg
      721 cattaatatt cgattcttac tatgatgctt acaaaggtgt tgtagcttat gttagagtat
      781 ttgaaggaac tgttaaaaag ggtatgacaa taaaaatgat gaataccaat aaaaagtttg
      841 aggtaactga agttggtgtt atggctccag gtcaaactga acttagtgaa ttatcagcag
      901 gtgatgttgg ttatattgct gctagtataa aagatataag aagttgtcgt gttggtgata
      961 caataacaga ttcaaataat cctacagaag aacctttacc aggatacaaa aaagctactc
     1021 caatggtata ttgtggtata tatccaggag aaggagaaaa gtatgagaat gttagagatg
     1081 ctcttgaaaa gcttcaagta aatgatgctg cacttgaata tgaagctgag acttcagcag
     1141 cactaggatt tggttttaga tgtggtttct taggtctttt acatatggag ataatgcaag
     1201 aaagacttga gagagaattt aaccttgata taattactac agctccatct gttatatata
     1261 gagttacaaa aatggatgga gaggttgtaa tgatacaaaa tcctgcaaac ttaccagaac
     1321 catctgagat aaagatgata gaagagccta tagtaaaagg agatataata gttcctaaag
     1381 actatgtggg tgttgttatg gagctttgcc aggaaagacg tggaaatatg ttaaatatgg
     1441 aatatataga tgatagaaga gtaatgcttc actatgacct tcctctcaat gaagttgttt
     1501 acgatttctt tgatgcttta aaatcaagaa ctagaggtta tggttctctt gattatgagg
     1561 ttaaaggtta tgtagcatct acacttgtaa aacttgatat attaatcaat aaagagcaag
     1621 tggatgcact tagttttatt gtccatgaga cacgtgcttt ccctagagga aaggcaatgt
     1681 gtgagaaatt aaaaggagaa attccaagac atcagtttgc tataccaata caagctgctg
     1741 ttggtaataa ggttattgca agagaaacaa taagcgctct aagaaaagat gttcttgcta
     1801 aatgttatgg tggagatata tctcgtaaga agaaacttct tgaaaaacaa aaagaaggta
     1861 agaagcgtat gagacaaatt ggttctgttg aagttcctca aaaagcattt atgtctgtgc
     1921 ttaaattaga tgagtaaatc cttataaatt tagataaaga tatttaaaag ctagagtgat
     1981 tttagaatta tttctaaatt agtctagctt ttttatggta tattttaagt aaactcatag
     2041 agaatataag taagaatatt aaagaagaat aaaaaggtga tatttatgat taatatagaa
     2101 attcctaaaa aagtagacta tataattaaa gaattagaaa agaatggtta tgaagcttat
     2161 atagttggag gatgtgttag agattgttta ttagaaagag ttccaaacga ttgggatata
     2221 acaactagtg ctagaccaga agtggtagta gaattatttg aaaaaacaat tccaactggt
     2281 atacaacatg ggactgtaac agttatgatt gaacatgaac catttgaagt gacgacttat
     2341 agaattgatg gaaattatag tgatggtaga catccagatt caatagaatt tacaaataac
     2401 atagttaagg acttgtcaag aagagatttt actatcaatt caattgcata caattcaaag
     2461 acaggattag tcgacccatt taatggctat gaagatatac aaaataagta tatacgttgt
     2521 gttggaaatc cagtagatag atttgaagaa gatgcactta gaatgcttag agcagttaga
     2581 ttttcagcac aactcaattt taaaatagct gaaggtacta agcaaagtat tcataaaaaa
     2641 gcagatttga taaaaaatgt ttctattgaa agaatccaga cagagtttaa taaaatatta
     2701 gtatctgatt catctaaatt aaacttatta aaatcaacag gtttgttgaa atttataatt
     2761 cctgaaatat gtgaattaga agatgttact caacacaatc catatcacat ctatgatgta
     2821 caaaaacata ctttaatagc tacagaagta attgaagatg aactgtattt aaaacttaca
     2881 atgttgtttc atgatttagg taaaaaagta actaaaacaa ctgataaaaa tggtgttgac
     2941 catttttata ctcatagtag agaatctgta aagattgcaa agaagatttt gaaaagactt
     3001 aagtatgata attatactat taataaagtt ttaatattaa tacaatatca tgattataga
     3061 atagaaccta aaaggaagat aattaaaaag ttattaaata aacttgaaga tgttgaatta
     3121 tttgaagatt taataaaagt taattgggct gatacacttg caaaaaatcc aaagtatgca
     3181 aaacaaaaga tattaaatct tattgagtgt gaaaaagaat ttaaacatat aataaatcaa
     3241 aaggagtgct ttaatcttaa agatttagct attaatggaa aagatttgat atctataggt
     3301 ataaaaccag gtaaagatat aggatatatt ttaaataaga tgcttgaaat agtcataaat
     3361 aatccagagt taaatgaaaa agaaatacta aaagaaaaag cattaaatat atatacattt
     3421 tagattcaaa tataaaaaat ttaattactt taaaaaacat agaataaaat acttatttag
     3481 ataaaataaa taatttttag agaatatttt agaagttacg attgtatgat ttttaaatgc
     3541 ataggcattt actctaggac tatagactaa tataaaaaaa aactttggga atactaaaac
     3601 cataaattta taaaattaat attttatata ctgtaaaaca gtgaggaggg acagtatgca
     3661 tactcaacta caaaagacta ttggtctttc agcagctctt tctactgttg taggtatggt
     3721 tataggttcg ggagtttttt ttaagccaca agcaatatac actacaacta atggagctcc
     3781 aggtctaggt attatagcgt ggcttttagg tggttttata acaataactg ctgggcttac
     3841 agctacagaa atttcagctg ctataccaaa aactggtggt atgatgattt atatcgaaga
     3901 aatatatgga gaaaagttag gttttttaac tggatggatg caaactgtat tatttttccc
     3961 aggtacatca gcggcattag gagtaatatt tgctcaacaa gcatctgaac ttttaggaat
     4021 gagcccaaat aatatggcaa atgtacttcc tatagcaatt ggagttatac tatttttagc
     4081 gttattaaat ataataggtt catccttagg aggaaaagtt cagacagtag ccactattgg
     4141 aaagatgata cctttaattt taataatagt ctttggattt attaaaggtc aaagtagtga
     4201 agtattaaat ccatttgtag gagatggtgt aaatgcatca aatgcacttg gacaagtatt
     4261 aatagctact ttgtttgcat atgatggttg gataaatgtt ggagctatat caggagaaat
     4321 gaagtctcca gagaaagatt taccaagagc aatagttggt ggtttatcat tagttatggc
     4381 agtttatatt ataataaata tagcttatct ttgggtagta ccagcaagtg aattagcaac
     4441 agttacttcc ccagctacat tagtagcaac aagattgttt ggaaatatag gtggaaaagt
     4501 aataactgtg ggtatattaa tatcagtatt tgggacattg aatggatatt tattaacagg
     4561 ttcaaggata ccttatacat tagctgaaat gggaacttta ccagcatcta aaactctttt
     4621 aaaagtaaat tctggaggtt ctcctgtaaa ttcaatacta cttataactg tgttagcctg
     4681 tgtatatgca ttatctggtc aatttaatct tttgacagac ttaacaatat tttcaatatg
     4741 ggtattttat gtattgactt ttataggtgt tatgaggctt agaagacaaa agccagattt
     4801 acacagacca tataaagtcc ctttatatcc aataatacca attatagcta tattaggtgg
     4861 actgtttgta ataataaatc aaatattgac ttcaacagtc atatctttag gcggaatatt
     4921 tataacacta ttaggtttac cagtttatta ttatatgaaa aaaagataac tacttaattt
     4981 aagttagata aatttgggta aaggtgaaaa ttaactttta tccaaatttt ttatactgtt
     5041 atacaatata agtattatat attgcttgat gtgctatttt gtttgattta taatttagta
     5101 attgatgttt taagataatt taagaaatga ggagtgaaag atgctaggac tttatgtaca
     5161 tattccattt tgtgtgaaaa aatgtaagta ttgtgatttt aattcttata aaatggacat
     5221 agattcaaaa aaaagatata tagaagactt aaaaatagaa atggaattat atagtaataa
     5281 attatataaa gataataaat ataaaaataa agaatgctgt agtctaaata aaaatgacaa
     5341 aataacgagt atatttgtag ggggaggtac acctagtata ctaacctctg atgaaataag
     5401 agaagtattt ataagtataa aagaaatgtt tgatatagat gagaatgcag aaataactat
     5461 agagtgtaat cctgggacat taacattaga aaaattaaaa actatgaaag agattggcat
     5521 aaatagactt agtatagggc tacaagctat tcaagaaaag catttgaact ttataggaag
     5581 aatacatact tatgaagaat ttgagaaaaa ctataaagat gcattaagtg taggttttaa
     5641 aaatattaat atagatttaa tgtacagtct tccaaatcaa actctatgtg attggaagga
     5701 aactcttgaa aaagttgtac acttaaatcc aacacatata tcagcttatt cccttatact
     5761 agaagaagga actgaactat acaatatgta tgaaagtaat aaatttgaac ttatagatga
     5821 aaatgtagat atagaaatgt atgagtatac aataaactac ttaaagtcaa aaggatataa
     5881 tcaatatgaa atatccaact actcaaaaga aggctataat tgtgagcata atattttata
     5941 ttgggaatgt gaacattata tagggattgg agcaggagct tctggctata ttaatgagaa
     6001 cagatacaat aatgtagaat ctttagaaga ttatcattta agtttggtaa aaagagaaaa
     6061 accaatacaa gaaaatgaaa ttctttctga gaaagatatg atagaagaaa agatatttat
     6121 ggggcttaga atgaataaag gcataaaatt tgaagatttt aaaaaaaaat ttggaataga
     6181 ttttagagag aagtataata agcaaataga aatgctatta gctagaaagc ttataaatca
     6241 gagctttgaa ggtatacaat taactcaaaa aggaagagaa atatctaata gtgtatttat
     6301 agaatttatg gaataatttt taaggtgttg acaaaataaa tttataatgg tatataatat
     6361 atataatctt tttagcactc gaacttagtg agtgctaaca gctaggaggt ataataatat
     6421 ggaattaaat gaaagaaaat taaatatcct caaagctata gttaaagatt atatagaaac
     6481 agctgaagct ataggttcta gaacaatatc taaaagacat gatttaggtg ttagtgctgc
     6541 taccataaga aatgaaatgg ctgaccttga agaattaggg tatttaattc agcctcatac
     6601 ttctgcgggt agagttccat ctgaaaaagg ctataagtta tatgtaaact ctcttatgag
     6661 taaaagcgaa ttggatgata atgataagat tcttattgaa caatgtatga atcacaatat
     6721 aaatcatata aaagaattga ttcatgaaac ttctaagcta ttatcgcaac taacaaacta
     6781 tacaactgta gcagttacta agagtttaat aaatcaaagt gttataaagc acatacagtt
     6841 ggttgctatg aatgacaata atattttact tatagttgta acagataaag gggacttaaa
     6901 aaaagctaac cttacaacta atgtatattt agatcaatct aagttaaatt tgatttctga
     6961 taatcttaca aggaagctct taggtaaaag tataacagat ttggatgata atctaattgc
     7021 ttttattaaa tacgaaataa gtgagtattc tgggcttata gatgagcttt taaatgcttt
     7081 gaactcaaat atgaaagaag aagatttttc tctatcatta aatggagcta ctaacatatt
     7141 tagttatcca gaatttaatg atgtattaaa agcaaagtca tttttaaata tgttggaaaa
     7201 aaaggaaaca atagctgaca taattaaatc aaagggaata caaaaagaca atctaaatat
     7261 aataattggt agtgataatg attgtgaact ggcacaggat tgtagtatag ttacagcaac
     7321 ttataatgta gatagagact tggttgggcg aataagcttt ataggcccta ccagaatgga
     7381 ttatgctaga atttactcta taataaatta tatgagttta ttaattaata gaaagtaaag
     7441 aaattttaaa ttgcgaggtg taaagtcttg gataaaaaaa atgagcaaca ggaagtaaga
     7501 gaagaaaatg atacttctat taaccaagaa tcggaaactc aagtagagtt agaagaggaa
     7561 gttgttaatg aagaatgtga aacatcaagt gaaaaaactg atgaaaaaga agttgatgat
     7621 gaaaatgtaa cagatataaa ttctaagtta gcagaaaaaa aattgcagga tgagttggat
     7681 gaattaaatg ataaatatca aagacttcaa gccgaatatg caaactacag aagaagaact
     7741 caacaagaga aagagacaat aggcgtattt gcaaatgaaa aaataataac tgaattgata
     7801 ccagttatag atagtatgga aagagcctta gatgcatgtg aagataagga agatactatg
     7861 tataagggta taagccttgt acataaacaa ttaatagaca ctttagtaaa atttggagta
     7921 gaagaaatag aagcagaatc taaggaattt gaccctaatc tacatctagc agttatgcaa
     7981 gaaagtgtag atggagttga agcaaatcaa atagtaatgg tacttcaaaa aggatataag
     8041 ctaggaacta aagttgtaag accttcaatg gttaaagttt cttgctaagt ataaattaaa
     8101 ggttaaatta tttaacctaa tataaaatat taaaataagt aaaacttaat tatataatta
     8161 ctcgaggagg attttagtca tgggaaaaat aataggaata gatttaggaa caacaaattc
     8221 atgtgttgca gtgttagaag gtggagaagc acaaataata gcaaatagtg aaggtatgag
     8281 aactactcca tcagtagtag catttacaaa agatggtgaa agaatagttg gagaacctgc
     8341 aaaaagacaa gcagttacaa atgcagataa aacaataact tctataaaaa ctcatatggg
     8401 aactgattat aaagtaaata tagatggaaa atcatatact ccacaagaaa tatcagcaat
     8461 aatattacaa aaattaaaat cagatgcaga aagttactta ggacaaacag taacagaagc
     8521 agttataaca gtaccagctt actttacaga tgctcaaaga caagcaacta aagatgctgg
     8581 tagaatagca gggcttgatg ttaagagaat aataaatgag ccaacagcag cagctcttgc
     8641 ttatggtatg gataaattag accaagaaaa gaaaatatta gtatttgact taggtggagg
     8701 aacttttgac gtatctatac ttgaaatagg agatggaact tttgaagttt tagctacagc
     8761 tggtaacaat agattaggtg gagatgactt tgaccaaata gtaatagatt atttagcaga
     8821 ggaatttaaa aaagctgaag gtgtagattt aagaaatgat aaaatggctc ttcaaagatt
     8881 aaaagaagca gcagaaaagg ctaagaaaga attatcatca acaatgagtt caaacataaa
     8941 cttacctttc ataactgcta cagcagaagg accaaaacac ttaaatatag atttatcaag
     9001 agctaaattt gaagaattaa ctagaggttt agttgaaaaa actatggaac caactaagac
     9061 agctttacaa gatgcaggac tttctacagg tgatatagat gatgtattat tagtaggtgg
     9121 ttctacaaga ataccagcag tacaagaagc tgttaagaag tttataggaa aagaacctca
     9181 taaaggtata aacccagatg agtgtgttgc agcaggtgct tctatacaag ctggagtttt
     9241 agcaggggat gttaaagact tattattact agatgttact ccattatctc ttggtataga
     9301 aactatgggt aatgtaatga ctaagataat agaaagaaat actacaatac caactaagaa
     9361 gtctcaaata ttctcaactg ctgctgataa ccaaacagct gttgatatac atgttcttca
     9421 aggtgaaaga agtatggctt atgacaatac aactttagga agattccaat taacagatat
     9481 accaccagca caaagaggaa tacctcaaat agaagttact tttgatatag atgcaaatgg
     9541 tatagttaat gtttcagcta aagatttagg tacaggaaaa gagcaaaaaa taactataac
     9601 atcaaacact aatttatcag aatcagaaat agagcaaaag ataaaagaag cagaaatgaa
     9661 tgcagaagct gacaaacaaa agaaagaaaa aatagaagca ttcaatcaag ctgaatctac
     9721 tatatatcaa actgaaaaaa ctttaaatga acttggagat aaaataagtt caggtgaaaa
     9781 agaggatata gaaaaagcta tagcagactt aaaagctgta aaagataatc aagatgctac
     9841 agcagaagaa cttaagaaag ctacagatga agtaatgact aagttccaaa aagtatctca
     9901 agaaatgtac caaaaagcag ctcaagaaca acaagcggca caaggtgcag aacaagcaca
     9961 agacaatgga cctaaagatg acaatgtagt agatgctgac tttaaagaag tagatgaaga
    10021 taaataagct atataatatg taacactaaa tgataattgg aacatatatg atttgtgaaa
    10081 atcatataga tgttaaagat atatggacaa tatacatcat aaagaatata atatatcata
    10141 gattatataa cttaaagtag aatataagaa ttaatgatag cttgtagttt attattctac
    10201 aagctatcgt tttgtaaaat gaaggtggtg tcaatttttg agtacaaaaa gggattatta
    10261 tgaagtctta ggtatcagta aaggtgcaga agctcaagag ataaaaaaag cttatagaaa
    10321 attagcaatg aagtaccatc cagatagaaa tcctggagat aaagaggcgg aagaaaaatt
    10381 taaagagata aatgaagctt atgaagttct atctgatgat acaaagagaa aaacttatga
    10441 ccagtttgga catgatgggc taaatggtca aggcggtttt ggtggtcaag gaggatttgg
    10501 cggtcaaggc tttggtggtt ttgaagatat gttcggagat atatttggtg acatgtttgg
    10561 aggaagcttc ggaggtggaa gagcaagaag aagagggcct caacgtggtg cagatataag
    10621 acagagtgtt actatatctt ttgaagaagc tgcatttggt aaaaagatgt ctataaaagt
    10681 aaatagaagt gaagaatgtg aagaatgtaa tggaactgga gcaaaaccag gaacatcaaa
    10741 gaaaacttgt tcaacttgta atggaactgg tcaagttaga actgttcaaa gaactccatt
    10801 tggtaatata gcaagttcaa gaccatgtag tgcttgtaat ggaactggag aagtgattga
    10861 atctccatgt agtaaatgtc atggaactgg aaatacaaga aaagttaaga ctatagaagt
    10921 agatatacct gctggtatag atgatggtca aatgataaaa ctttctggtc aaggtgaagt
    10981 aggagaaaaa ggtgctccaa gaggagactt atatattgta gttaatgtta agtcacatcc
    11041 actatttact agagatggaa atgatattta ttttgaaatg ccaattacat ttgttcaagc
    11101 aactttaggt gatgagatag aagtacctac tttggatgga aaagttaaat atagtgtgcc
    11161 agaggggaca caaactggaa ctgtatttag acttaaagag aaaggaatcc ctagaattag
    11221 aggtaattca agaggagacc aatatgttaa ggttgtagtt gaaattccta aaaaattaaa
    11281 cgataagcaa aaagaactat tgagagaatt tgcgaaggag tgtgggtcga acgtccacga
    11341 aaagaagaaa accttcggac aaaagataga agatatgttt aagaaaaagt agttaaagta
    11401 gccaatatat aatcatattt ttaaaatctt agttatttat aagattgtca aaggagatat
    11461 tatgattttt atattggcta tttttgtata aaaaataaat gtgcataaaa taaaactgaa
    11521 tataaataat tttctgaaaa atacttgtcc taaaatatat aatatgctat aatataatca
    11581 aataaaagaa aggggtgatt ccagtgggaa cggatgtggc actgccaaat gatgaagaaa
    11641 actttattgt aatatacaaa ataaaattag atacttttag tgctagatta agtactggaa
    11701 aaactcatga gctgggataa ttattgtaat tacctagact caaactttta gtagtaaaat
    11761 agtaatatta tatagacaag gtatctaatt tttaaatctt ttgtatgaat taaaattttt
    11821 ataaagggta tatctgatta gaatgtaatt agggaaacct agttgtgaat tacttttagt
    11881 atatacatgt gtaaagaaat atgtatatgg tattgggtat tgtgctagat gtgtatgtaa
    11941 aggaatatgt gtctagttgg gtatatgtaa agaaatatac ttataatcta tgaaaaattt
    12001 ttagtataaa ttgctaaaac attgagattg ttttttatag aaagttagtt tataccaact
    12061 aaaaatgtgc ttaagatttg tttgatgttt aattataaat aaggttgtag ataaaaaaat
    12121 aagtaaagac aaaggtttaa aatacttgct ttaaatatag tttgttttga acacttgcca
    12181 aattaaaata attaataaaa tagacatctt ctcatagtaa aatatactgt gagaggatgt
    12241 ttttgctttg ttcataaact tttatgaaaa atttgcatag tgtcacattt taatatacta
    12301 tatatataat aaaatataga taaaattaaa tgattcaaaa gagaaaggtg aaaagagatg
    12361 tattatatag gtgtagacat aggaggaaca ggtatacaag caggagtagt tgataactat
    12421 gggaagataa tttttaggtc agagtgtaaa acagtaattg aaaaaggatt tgaaggaata
    12481 cttaatgaca taaaaataat gatttataag ttgttggaag ataataaact tactatgagt
    12541 gacataaaat ctattggatt tggtgttcca ggttttataa ataaagaagg attagtaact
    12601 tgtgtaaatt taaaatggaa taaaaaagct tttaataaag agttaaaaag aagatttcca
    12661 gatgttgaaa ttcatggaga aaatgatgca acagtagcag cacttggaga agcaaagttt
    12721 ggaagtatga aaggtgctaa tgttggagta ttatatactt taggaactgg tgtaggtgga
    12781 ggaatagtaa ttaatcaaaa agtcttttct ggagcacatg gattgggttc agaaataggt
    12841 catcaaatta taggagaaaa ctattttaat tgtaattgtg gaaataatgg ttgtgtagaa
    12901 actttttgtt cagcaactgc aattataaag tattctcaaa aactgataga agaaggagaa
    12961 aagagtagga ttttagattt ggcagaggga aatttagaaa atgtaaatgc aaagatggtg
    13021 tttgatgcat atagagaaaa tgatttagtg gcaattaaag taataaatag atttaaagag
    13081 tatttagcaa aaacttttgc aaacacaata aattctttag acccagaaat tatatctata
    13141 ggaggaggca tttctaaatc tagcgatata atacttgatg gaatagaagg tttggtaaga
    13201 aaatttgtat tatacaaaac agaagatata gctacaatta catgtgcaac tttaggttca
    13261 gacgcaggaa ttataggagc agctttttta taatgtaaat gaactgctta ttggaggcgt
    13321 gatgatatat gggtaaaaca aatgctagaa gaacgctttt tttgatagca ataggttcag
    13381 gaactatgct gaatcctctt aattcttcta tgatttcact tgctttacat agtatacaaa
    13441 atgattttca tattagtttt acaactgtat cttggattgt ttcagcattt tacctagcta
    13501 gtgctattgc acaaccagtt tcaggtaaga taggagattt aataggtaga aaggtcttat
    13561 ttttgagtgg tttgatgctt gtaattttat cagcatccat agtaccactt gttcaatcat
    13621 tctttatatt gatttttata agagtaattc aagcaattgg aacaagtacc ctatatccat
    13681 ctggagttgc tcttatacaa aataatataa aagaaagaca atcttctgca ttagcagttc
    13741 taaccatttt tgcttcaact acagcaggat taggtccgac acttggagga ttattgttgg
    13801 acttaggagg atggcatgct atatttcttg taaatatacc tgttgttttg gtttctatat
    13861 gcttagggta ttttttattc ccaaaagaag taaaagaaaa gaaatctata aaagaaactt
    13921 taaaaaatgt tttgtcaaga ttagatatta ttggcatact tctattttca attgcaatga
    13981 tttttatatt actatttttg ctatctatga aggaatcttt taacttagaa caacttgtat
    14041 ttggtataat actgatgtgt ctttttattt ggcatgaatt gagaacaaaa ttgccattta
    14101 tagatattaa gctttttgta agtaatccta aattgtcaaa agtttacttg caatttatta
    14161 tattaaattt ctttaattat attttgtttt ttggtcttcc tagttatttt caagatgcct
    14221 tacattatag tgcaaagtcg actggattat ttatgctatt tatgtctggt ataggtatat
    14281 ttatatctcc attaactgga aaatggatag ataaatctgg aactagattt cctcttgtta
    14341 catcatcaat ctttatgttt attagtgcag tacttctttc agtagttttt atccatatac
    14401 ctgtaatagg aaagggtatt atattatcat tagcaggtat tagttatgga gttggtaatg
    14461 tagccctgca atcctctatg tttgaagaaa gtcctcgtga cagtataggg acagcttctg
    14521 gtctgtttca aactagtcgt tatttaggtt ctattttagc aactgtttta cttggaatgg
    14581 tatttgaagt aaatataaca tcagaacaat ttcagatatt aggttatgtt atggtggtct
    14641 taggtacaat aagtttttta ttaaatttta tattaaagaa agaattaaga aaagtagaat
    14701 agtggattta tggatttaat aagtctaata actgataagt ttaaaaatat tgtttgtaaa
    14761 acataactgg tttgatattt attaattcta agttttgaag catagagtac tcgataagta
    14821 tacaatattt tgaattaaaa ttaagcagaa atttgaacta gtctttcaaa tctctgctta
    14881 atttagtagt taaatttttt tatagagaaa ataagatttg acacttagat aaaaaatcat
    14941 tgcattttca aaattcttta aatcaaaccc aagtatctca ttaatctttt ccaatctata
    15001 tactagcgtg tttctatgta aaaacatact agtagatgtc ttagaaatat ttaaatcatt
    15061 atttataaat tttattcctg tatatagaag ttcttcgtca atagatttaa tcttattaaa
    15121 atctattaat ttattgagta tattatcctc attaattaaa tctaaagttt tatatctaat
    15181 aaggtcaaac aaatttaaaa acttattttc agcgttgtct tttataatat gaggtagtaa
    15241 atcagaacat aactgtaatc taaaatcaaa atcagcttta ctatctaatt caccaagata
    15301 aaaaatataa gtactttgat aagtttcagt ttcaattata ttttgtgtag ttacattaat
    15361 atctaagtta tatttagaat ttactataaa aaaaccattg tgttgtatta tatttatatc
    15421 ttcaaataaa ttataaaaaa tattttttag atattcaaag tctatatgag attggtaaat
    15481 taaataaaag ttgcctttta aattatcgag attattttta tctagaaaat caataacata
    15541 aatagcatta ttgaaagtaa ctaaatggct actagcatct aacttttggt tttcatatgg
    15601 atagatattt atttttttat taagctgtct ttctaaaaaa tcttttaact cttccataaa
    15661 gtctccttaa aaataagatt atattcttat cttatagcta aagtagtttc tttgtcaaat
    15721 atgtgtactc tactattgtc aactgaaagt gttaagtcat caccaacatt aattctgttt
    15781 ttactgtcaa atctaattgt tatcgtattt tcgcctagtt ttaagtgagc ataagtttct
    15841 gctcccataa gttcttttat ttcaattctt gatttaaaac aagtatctgg attagattta
    15901 ataaaagtat cttctacatg aatatcttct ggtctgatac ctataacaac ttcattatta
    15961 atataattgt cttttattaa atcatagtgt ttttctttat tcaatttaag cttaatatgt
    16021 tcattttgtg cataaatttc atcattttct tctattaata ttacatcaat aaaattcatc
    16081 tgtggtgctc caataaatcc tgcaacaaat atattagctg gataatcgta tacatcttgt
    16141 ggagttgcaa tctgctgaac tactccatcc ttcattacta cgattctatc agccatagtc
    16201 atagcttcaa cttggtcgtg tgttacatag ataaatgtag ttccaagctg ttggtgtagc
    16261 ttagttatct ctgttctcat agcagttcta agttttgcat ctaagttgga tagaggttca
    16321 tccattaaga ataccttagg atttctaact attgcacgtc ctaaggcaac cctttgtctt
    16381 tgtcctccag ataatgtttt aggtttttta tttaaaagag gtagtaagtc taatatttta
    16441 gcagcttctt taactttttt gtctatttca tctttaggca attttctaag tttaagtgca
    16501 aatgccatgt tttcgtaaac agataagtgg ggatataaag catagttttg aaaaaccata
    16561 gctatatctc tatcttttgg ttctatatca ttgacaagtt tatctccaat gtatagttct
    16621 ccttcagaga tttcttcaag accagcaatc attctaagtg tagtcgattt accacaacca
    16681 gatggtccta ctaaaacgat aaattcttta tctttaatgt ctatatttat attttttact
    16741 gcattaaatc catttgagta aagttttgat atatttttta aaattacttc tgacataata
    16801 tcctccttga tatttaaatt ttaattatat agtatcaaat ttttttatta tattaaatgt
    16861 gtaaaatgac taaatgtatt tgtttaatat gtatagttag tttttatatt aagatttatt
    16921 ataatttaga aaatatattt taattttttt aatttttatg tataataata tatatatttt
    16981 ttagattaac aatatgggat ttaaattatt ttttaattta taatgaaata atattatatt
    17041 agtagttttg gttaacaatg aggagtttat aaataaggtc atataagtta aattatatcg
    17101 attttattta aataacccca tatttattaa ttattagaaa tatattttaa ttattgacta
    17161 aataataaat actgtaatat aatggaaata cagtttaata aatataaaaa gagaaagagg
    17221 gaattttaaa tgaatttgaa gaaaaaagag aaaatagtta agcctgtttt aaagaagctt
    17281 acagaaatag tattaaatat attatcaaaa aaaatattaa actatgctat atatgcaatt
    17341 tctgttttga tagtaacaaa agttgttata aatatagatt tgtacatatc catattgtat
    17401 tattag
//
LOCUS       DAEMGG010000082        17386 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEMGG010000082 DAEMGG010000000
VERSION     DAEMGG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.082"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1527
                     /locus_tag="KPK24_002505"
     CDS             <1..1527
                     /locus_tag="KPK24_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2300172.1"
                     /translation="AKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVEEVSF
                     FKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFCKCII
                     SLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKEAEVI
                     LLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYKSFGD
                     VFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYGLIAN
                     GYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLVDDNK
                     KLENDILKDGLTKLYNRRYLEQQLDLFNETRDKNMVAILVDIDCFKSYNDNYGHLAGD
                     KVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRVFKLL
                     EFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQYIII
                     "
     gene            1535..3535
                     /locus_tag="KPK24_002506"
     CDS             1535..3535
                     /locus_tag="KPK24_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2300173.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEIVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            3797..5056
                     /locus_tag="KPK24_002507"
     CDS             3797..5056
                     /locus_tag="KPK24_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2300174.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            5117..6904
                     /gene="iorA"
                     /locus_tag="KPK24_002508"
     CDS             5117..6904
                     /gene="iorA"
                     /locus_tag="KPK24_002508"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG2300175.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            6906..7481
                     /locus_tag="KPK24_002509"
     CDS             6906..7481
                     /locus_tag="KPK24_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG2300176.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            7534..8604
                     /gene="buk"
                     /locus_tag="KPK24_002510"
     CDS             7534..8604
                     /gene="buk"
                     /locus_tag="KPK24_002510"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG2300177.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            8822..9523
                     /locus_tag="KPK24_002511"
     CDS             8822..9523
                     /locus_tag="KPK24_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300178.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            9583..10089
                     /locus_tag="KPK24_002512"
     CDS             9583..10089
                     /locus_tag="KPK24_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG2300179.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            10264..11286
                     /locus_tag="KPK24_002513"
     CDS             10264..11286
                     /locus_tag="KPK24_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2300180.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(11433..11741)
                     /locus_tag="KPK24_002514"
     CDS             complement(11433..11741)
                     /locus_tag="KPK24_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG2300181.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(11801..12229)
                     /locus_tag="KPK24_002515"
     CDS             complement(11801..12229)
                     /locus_tag="KPK24_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300182.1"
                     /translation="MNNKLHELEKNLPEISCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(12452..13909)
                     /locus_tag="KPK24_002516"
     CDS             complement(12452..13909)
                     /locus_tag="KPK24_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG2300183.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            14327..15241
                     /gene="nadA"
                     /locus_tag="KPK24_002517"
     CDS             14327..15241
                     /gene="nadA"
                     /locus_tag="KPK24_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG2300184.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEGVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            15254..16555
                     /locus_tag="KPK24_002518"
     CDS             15254..16555
                     /locus_tag="KPK24_002518"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG2300185.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            16536..17372
                     /gene="nadC"
                     /locus_tag="KPK24_002519"
     CDS             16536..17372
                     /gene="nadC"
                     /locus_tag="KPK24_002519"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG2300186.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
BASE COUNT         6769 a         1837 c         3178 g         5602 t
ORIGIN      
        1 gcaaaaaaaa ttataagaca aaatcattta aatgagttgt tggtattgtt aaatatgagt
       61 ttatgtgtag aaataagtgc tttatatcag accaaaacgg atgtaatagc tttagtagaa
      121 gaagtgtctt tttttaaaac tttaaaaaat aaagctcttg taagtagggc aaattatata
      181 tttgggagag tttatctata tttatttaat aactttataa aaagtatgga atacttaatt
      241 gaatgtctaa aattggctca agaaaataat ttatactcta tagaggcttt ttgtaaatgt
      301 ataataagtt tgtgttattt agagagtgga aataatgtag aagctataaa atacataaat
      361 aatgtattag atgttgcaca cagtaataac tcaatatcag taactgaaag agtgtctata
      421 aaaatagacc ttatatgggc atatttaaaa gaagatatga ataaagaagc agaagttatt
      481 ttattaaaga ctataaaatt aatgaaagtt gttgaaggtg tatataagga ttatttatat
      541 tcatttatat ttttatattc agctgaaatt gagttaaaga aagataattg tagttttgaa
      601 aaggtaaaca cctatttaat tttatcaaaa aatatatata aaagttttgg agatgttttt
      661 atatataatt ctaaactaga ttacatatat aagttgtatg gagatttata tataaaattt
      721 ggtaatatag atgaaggtat aagttgttat ataaaaggtt taaatttagt taaaaagagt
      781 tcattgaatc ttagaaaaat atctatattt tatgggctaa tagcaaatgg atatgagcta
      841 aaaaaagatt ttaaactggc aatagaatat tataaaagta tggattggta ttctgatagg
      901 tgggaaaaaa ataattccta taaaacatct caagcaattc acaaacaata tgaactcaga
      961 caaaaacaag agagtttaag attactagtg gatgataata agaaattaga aaatgatatt
     1021 ttaaaagatg gacttactaa actatataat agaagatatc tggaacagca gttggattta
     1081 tttaatgaaa ccagagacaa aaatatggtc gcaatactgg tcgatataga ttgttttaaa
     1141 agttataatg ataattatgg tcatctagca ggagataaag ttatagttaa ggtaactgat
     1201 attataagaa gtgtatttat agatattacc gaacacataa tcagatatgg gggagaagaa
     1261 attttaatat tagtagattc tatagaatca atcggtgttg aagagattga taatatagaa
     1321 ttatatatta aaagagtttt caaacttctt gaatttgagg gtattgaaca tatatattct
     1381 aaaataaaaa actatataac tataagtgct ggagtaagta taaaaagatg tcgtagtaga
     1441 aaagatgttg aaatattaat tgaagatgca gataagaacc tatataaatc aaaaaaagca
     1501 ggtagaaatc aatacattat aatttaaaat tgaggtgatt ttgttgcgaa aaaaaatagg
     1561 aattatagcc tctgatattg aattaaaaga aagaatagaa gaattatata gagaagatgt
     1621 agaaaatgga actataataa tcgacatact taacctagac ttaatggaaa atcaaggtcg
     1681 tatacttgtt gaaaaaggtg cccaagctat aattggtaga ggtggaggtt atagtttagt
     1741 aatagataca gtaaatgtac ctgttatacc aatgaatatg aagtcaactg accttttaag
     1801 agcaatagaa atagcaaaga aatattctaa aaaagtagta ttgattcttg gagacaatga
     1861 agtttctttt gactatgttg gctggagaaa tgtcataagt actgaaataa ctgaagaatg
     1921 gtttgaatct aagtatgaga taagaagtaa agttgtaaaa tatatagacc aaaaagatga
     1981 aattgtaatc gtaggaggag gtttggcatg ttcttttgca aggcaatatg gaattgatag
     2041 tgtatttgca actgctagtg atgaatctat aagagaagct gtagaatatt gtaaaaaatt
     2101 attagataca ttaggtgaag aaaagtttaa taatgaagtg ttacgaaata tacttgatgg
     2161 tataaaagat ggagtaattg ctatagatag taatggcagt ataattttgt acaatgaaag
     2221 tgcaaaaaac atgcttaaag tagagagaaa atgtgcactt aataaataca tcttagatgt
     2281 atttcctaaa atggagtgga tgttagattg tttacatgaa aaagaggacg tagaagatag
     2341 aaagataaga aatataaata atctaattgt taatactagg acaacattga taaaagttga
     2401 taatagtact tatggagttt taggaataat acaagatata accaagctac aaaacttaga
     2461 aagaaaaata agatttgact tgaatcaaaa aggtctctat gctagatata cttttgatga
     2521 ttttttattt aaagataaat taactaaaga atttattgaa gaagctaaaa aaattggaaa
     2581 aagtgattat acaactttac tttatgggga aagtgggtct ggtaaagaaa ttatagctca
     2641 tagtatacac aatataagta aaagaaaaga taggcctttt gttgctataa attgtgctac
     2701 tatagcagaa aatttacttg aaagtgaact ttttggctat gaagaaggtg catttactgg
     2761 tgctagaaag ggtggaaaaa gagggctatt tgaacttgct catggaggga ctcttttttt
     2821 agatgaaata aatagtctat cctttaatat tcagacaaaa ctattaagag taatagagga
     2881 acgtcaaata atgagaattg gttctgatta tataatacct ctggatataa ggattatagc
     2941 agcaacgaac gaatctttaa ccgaaaaaat agttatgggt acatttagag cagacttatt
     3001 ttatagattg agtagtttag agataaatat acctccttta agagacagaa gagaagatat
     3061 aatacctttg tttaacaatt ttgtaaatga agtattaaag gatgatggtc tcaatggaat
     3121 aaacagtata gatgaaaatt ttgttttgac taaagatgaa atggataagc tatataatta
     3181 tagctggcct ggaaatgtaa gagagttaaa gacaatagct caaaaatatg ttgtgacagg
     3241 taaaattaaa ttgagacaag atagagattt taaaactaaa aaattacttc ctaactctga
     3301 agtagacaaa tttaattctg aaacgacagc aagtgtagaa gtccaagatg aatctataaa
     3361 tataagtaag ataaatgatg gtaagataag tatagatata aaagaagtaa ataagtatgt
     3421 tgaagaaaaa ataataagta tgctttttgc tcaaggactt tctaaaaatg aagttgcaca
     3481 agttcttgga ataagcagga catctttatg gaagaaatac aataaaaata tttaaatata
     3541 attacatata aattattttg aagtaatgtt aattttttaa aacaagtgtt aagaaaaatt
     3601 aacattactt ttttgcaaat ttttattttt ttagacaggc atatatattt ataagaaaaa
     3661 tagttaaaac acttaattta ataatgaaaa aaataataat tctaaacaaa atcaatttta
     3721 cataaagtgg gatttattgg catagtaatt gcttaatata taatgtaaga gtaaatacaa
     3781 ttttaggggt gatggtatga gtcaaagtat ggttggtaag catgcaatgt ggccaaaaga
     3841 aaatgatgtt atatttagca tatctggcag agctcaagca gcagagaagg cttttggtat
     3901 ggataatgta ataaatgcaa caataggcgc tctaatggat gattcaggaa aattgataac
     3961 aatgaaaact gtatatgaag aatataaagc attagataac tgtgagatag gtgcatatgc
     4021 agctcttgaa ggtcaaccag attacttaga agcagttaaa aaagtatttt tcagagatta
     4081 cttaccagaa ggacatataa gagtattagc ttcaccagga ggaagtggtg caataaaatt
     4141 agctgtatgg aactatacta atgaaggaga tgaggtatta acatctgatt ggttctggag
     4201 tccatatgta agtatagctg aagaggctaa tagaaaagta gttaattacc aactttttga
     4261 tgaaaataga aggtttaatt ttgagtcgtt taaagaaaaa tttgtaaata tagcagaaaa
     4321 acaaggtaga gtctttacaa ttataaacac accagcgcat aatccaactg gatatagtgt
     4381 tgcagatgat gagtgggata aaatattaga cttatcaaaa gaagtcgcaa aagataaaga
     4441 caaaaagata atatttttcg tagattcagc atatattgac tttgcaggtg atgatgatgt
     4501 atgtagaaag ttttttaaga aattttctaa tttgcctgaa aacgttctcg tattggttgg
     4561 attcagtatg tcaaaaggat ttacagctta tggtatgaga atgggggcta taatttgtat
     4621 atcttcaagt gaagatgtag ctgaagagtt ccattactca tgtgttcatt catgtagagc
     4681 aaattggtca aattgtaata gaagtgccat ggcagtatta tctaacattg taaatgaccc
     4741 taaaaaattc aaagaatatg aggatgaaaa agaaatatac aaaaacatgc ttactagaag
     4801 agcagatgta tttgtaaaag aagctgaaag agtaggatta gaaattcttc catatatagc
     4861 aggtttcttt gtaagtatac catgtgataa tccaaaagaa gtatgtgaag aattgactaa
     4921 acataattta tttgcagttc cattaaaaat gggtttaaga tttgcagttt gtgcagtttc
     4981 tgaagataaa tgtaagaaag caccaagtat tataaaagaa gcacttgaaa gtttagaagt
     5041 aaaaataaat aattaatttc attgaacaaa ttgggtggac tatgaattta ataattgtag
     5101 atggagggaa ttttatatga aacaattgat gacaggtaat gaagcaattg cacgtggagc
     5161 ctatgaggct ggtgttaaat atgcatctgc atatccaggg actccaagta cagaaatatt
     5221 agaaaatata gcaacatata aagatgcaat agttgcagag tgggctccaa acgaaaaggt
     5281 agcacttgag gctgctatag ggggttcaat tgcaggagca agaacaatgg cttctatgaa
     5341 acatgttggt ttgaatgttg cagctgatcc aatatttact tatgcatata caggggtaaa
     5401 tggtggtatg gtacttatta cagcagatga gccgggaatg cattcatctc aaaatgagca
     5461 agacaatagg atgtatgcaa aatttgcaaa gataccttta tttgagccat caactagtca
     5521 agaagctaaa gatatgataa aagaagcttt tgaagttagt gaaaaatacg atacaccagt
     5581 tttatataga gttacaacaa gactttgtca ttcaaaagga ttagtggagt gctatgatag
     5641 agaagaagtt gaaattaaag agtatgttaa aaatgctaag aaaatggtaa ctgttccagc
     5701 aaatgctcaa ataagaaggg gcgttgttga agaaagaatg gaaattctta aaaaattctc
     5761 aaatgaaact gatttaaact actatgaaat aaatgataca aaaataggtg ttatagcctc
     5821 tggtatgtgc tgtaattttg ccaaagaagt ttttggaaaa aatgcatcat atatgaaact
     5881 aggatttaca aatccattac cttatgaaaa aataaaagaa tttgcagaaa aagttgataa
     5941 aatatatgtt atagaagaaa atgacccatt tatagaagaa caaataaaag catatggaat
     6001 agattgtatt ggaaaagatg taataccacc atatggagaa atgacaccag atgttttaag
     6061 aaaagctata tttggaaaaa caaatgatac tatagaatac aaatctgaat tagtaacacc
     6121 aagaccacca agtttctgtg caggatgtcc acatagagga ttcttctatg aacttggaaa
     6181 gagaaaaaat ttaatagttg gtggagatat aggttgttat actcttggat ttgctcctcc
     6241 atataatgga atagattatg ttgtttgtat gggttctgct tttggaactg cacatggagc
     6301 tcaaaaagtt cttaatatga aggatgataa tgaaaaaaga ttggtaggtg tacttggaga
     6361 ctcaacattc ttccatacag gaataaatgg acttttagat gttgtttaca atagaggtaa
     6421 ttctatttcg gttatattag ataatagaat aactggaatg acaggtcacc aagaaaaccc
     6481 aggttcagga tatactttac aaggtgctaa aactaaagaa gttgatatag aaggattagt
     6541 taaagcttgt ggaatagaac atgtaagagt tataaaccct aataatttaa aagaagtaaa
     6601 tgaagcacta gattgggctt tagctataga agatgaacca tcagtaataa taacaagatg
     6661 gccatgtgta cttaaaaaat tctctaaaga agatatagaa gaatttaata atccatttaa
     6721 gactaagtgt aaagttgacc atgataaatg tataggatgt aaattatgtt tgaaaactgg
     6781 atgtccagct ctatcatttg ataaagagaa taagttatca aatatagata gaaatcagtg
     6841 tgtaggatgt ggagtatgtg cacaagtttg tccaaaacaa gcaatagtta aggaggaaaa
     6901 ataatatgac taagagtata cttttggtag gtgtaggagg tcaaggaact atacttgcta
     6961 gtaaattatt aactatggga ttgatggaag ctggatatga tgtaaagatg agtgaaatac
     7021 atggaatgag tcaaagagga ggttcagttt cctcacaagt tagatatgga gattgtgttc
     7081 attctccagt tatagaaatt ggtggagcag atatattagt atcttttgaa aaaatggaag
     7141 ctttaagatg gttcaattat ttaaaaccag aaggaaaagc tttagtaaat aatcatagga
     7201 tagattctat gactgtttta atcggaggag ctgaatatca agaaagtgat atagatgcag
     7261 agttaagtag attaaatgca aaagttataa atgcagctga taaagctgaa gaactaggaa
     7321 attcaaaaat aatgaatgtt attttattag gatgtctagt taaatctatg caattagagt
     7381 ctattgattg ggaaaaaatt ataagtgaaa atgttaaacc aaaatttgtt gaattaaaca
     7441 taaaagcttt ccatgaaggt atggaaatgg ttagcaaata gtttataata gatataaata
     7501 gactatagaa attaaggtga aaggaagaaa attatgactt acagaatatt agccataaat
     7561 ccaggttcta cttctacaaa aatagcagta tatgatggag aagaacaaat tcttgtgaag
     7621 acgatagacc atccagctga agagattgca aaatataata ctatacaaga ccagtttgaa
     7681 atgcgtaagg aagcagtttt gaatattctt aaagaaaata gtatagactt aaaatctctt
     7741 agtgcaatag taggaagagg tggagtttta ccaccagtaa aatcaggagc atatttagta
     7801 aatgaagaaa tgattgatgt actaagacat agaccagtac ttgaacacgc ttccaattta
     7861 ggtgctgttg tggcacatgc aatatcagaa cctcttggaa tcaactcata tatttatgat
     7921 tctgttgcag tagatgagct tatagatgta gcgagaatat ctggactttg tggaatggat
     7981 agatcaagtg cagggcatgc attaaatact agagcaatgg ctttaaaata tgctaaggat
     8041 aaaggaaaag attataagag cttaaactta atagtagctc acattggtgg aggagtaagt
     8101 atttatcttc atgaaaaagg aagaatggtt gatatgctat ctgatgatga aggaccattt
     8161 tctccagaaa ggtcaggaag agtacctgct acaaaattag tggctgcctg ttattcaggt
     8221 caatattcag aaagagaaat gactaaaaag ataagaggta aaggtggtat agtttcatac
     8281 ctaaatactg tagatgctag agaagttgaa aaaatgatag cagaaggaaa tgaagaagca
     8341 aaaattattt atgaagcaat ggcttatcag ttagcaaaag gtattggaga gttagcaact
     8401 gtagtagatg gaaaggtaga tgctataatt ataacaggtg gaattgcata ttctgaaatg
     8461 tttacttcaa tggttaaaaa gaaagttgag tttatagcac cagtagaaat tatggcagga
     8521 gaaaatgagt tggaatcact tgcttttgga actttaagag tactaaatgg agaagaagaa
     8581 gctagaattt atagtgaaaa ttaataagaa aaactaaaaa tgtttatata ctaaaaaagt
     8641 ggtatatccg ttttttatac acgaaagctt agagtatagg tgaaaatgta taaaatttgg
     8701 attgccactt tttataatta aaattttatc ttaacctaat aaataattct gtaaaatttc
     8761 gtatttatat atatatttta ttggtattta ttgttattat tattatgagg agtgattttt
     8821 gatgggaaaa ataagtttaa aaagtatgat aagattattt tcaggttttt ttatatttgc
     8881 aatttcttct gtgcttatga ttaatgcaca tgtaggtctt atgccttggg atgtacttca
     8941 ccaaggttta tctataaagc taggcataac tataggtcag gcttctataa tggtaggagt
     9001 tgtaattgtt atattggatg cagtttttgg tgaaaatata ggatggggta ctttactcaa
     9061 catgacattt ataggaattt ttattgattt agtaatattt tctggagtga tacctcatgc
     9121 aagcaacact tatataggtg tgtttatggt tgttatagga attattttgg cagctatagc
     9181 aagttttcta tacttaggtg tttgtttagg aagtggaccg agagatgggc ttatgattgc
     9241 tcttcagaaa aaaactaata aatctgtacg tcttgttaga acaattcttg aaatactagc
     9301 attagttgtt ggatggttat taggaggttc tgtaggtatt ggtacgcttg taagtgcttt
     9361 aggtttagga tatgtattac aaatagtgtt tagaatattt aaatttgaca caaaattgct
     9421 gaaacataga tttattatag atgatataag agaatggaag gaaaagaaaa gcaatgaaca
     9481 taaatgtaaa tcaagtattg taataaaaaa tgagcagaat taattatgct aaaatatatt
     9541 tgtagtttat ctaatttata gtaaagtcaa ggagattttg aaatggaatt atgggattta
     9601 tataatgctg atggaattaa aacaggaaat gttataaaaa gaggtaattc tattgaagaa
     9661 ggttattacc atttggcagt tgaagtgtgg attttaaata gtaactctca aattttaatt
     9721 cagaaaaggt ctaaaagtaa aaaaacattg ccaaatatgt ggggaatgac aactggatgt
     9781 attgtttctg gggaagacag tttagaagga gcaattcgcg aagcaaagga agaaataggg
     9841 atagatataa ctaaagatga gatgaaagtt tttagaagta tgattcatga agatactctt
     9901 tgggatgtat acttagttaa aaaagaatat gatatatcta aagctatttt acaggaggaa
     9961 gaagtaagtg atataaagtg ggtaagcaca gatgaaataa gacaattact aaaagagggt
    10021 ttgttttttg aatatccaga gatatacgaa ttgctatatg agatagataa taataaatta
    10081 aatatatgat gtagagcaga tttcagtata agtaagatta tttaaaatgt atcttctcta
    10141 aattttaata ttgttttatg aaaattaata agttattttt agtatttttc tacttgtttt
    10201 ttgagattaa aataaaaagg gcagtcatta taaagtcata aaaatttggg gaggatttaa
    10261 actatggaag ataacaaagg attggcacgt gacagttcaa aatataaaat tgtttctata
    10321 tctgtcgtag taattattct aattacattt tggtatatgt taaatatggt gcttttaaca
    10381 tttattatga cttttatttt ctataacttg cttgtagcca caagaaaacg aattaagaag
    10441 ttttcttctt taaatattcc agattcattg ataattatag tgttatatgc actatttgct
    10501 atattgttag tattgattag ctatgcagtt gtaccaataa ttatagttca acttacagag
    10561 ttgagtaggg tttttagtga ttttgatgta aatcaatttg ctcaatcttt aggaccgaaa
    10621 ttgtatccta ttgtctcaaa gctggatttt aataaatata tttcacaagc agggctactt
    10681 atagcatcaa cagcaacaaa agttggaagt tttggagtaa atatattatt ggcattttta
    10741 ctgagtttac tccttttact tgaaaaaaat gaaattaaaa attttggaga taaactttct
    10801 gatagtaaga tttcttttat ttataattcg cttgtatttt ttggaaagag ctttgttaaa
    10861 aattttggtg aagtaatgaa agtacaagtt atgattgcct ttataaattc tgttgtttct
    10921 atgattttct taggtttcat gggattccct caaatatggg ctttaggatt tatgattttt
    10981 gtgttgggtc ttattccagt agcaggagtt atagtgtctt tgattccatt aactgtaata
    11041 gcatttaata caggaggaat aacaaaggtt tttggagttt tacttatgat ttgtatagtg
    11101 catgcagtag aaacatacat attaaaccct aagttgatgt cgaatagaac taaacttcca
    11161 gtttgttttg tttttattat tttacttgta ggagagcatt atttaggtgt atggggatta
    11221 ctaattggag ttccaatatt tatgttcctt atggatattc tgggtgttaa atttacttct
    11281 aggtaatata ttaaaataat aatttatttt tatatataaa ttatgaattg ttttaaaaaa
    11341 gaacctataa atagacatca ttaataaaga catctatttg ataggttcaa ttttgctttg
    11401 tataaatact ataaaactaa ataaaaagaa atttatttca tttcaaaagt ttcacaactt
    11461 gcatttcttt catttatatg aactttttca gctttgcaaa gttcattttt attgtattta
    11521 cattttacag cttcacaatg aatgttatct gtatcaactt ggttagtttc attagaacga
    11581 ctagttatac cagatgtata tttgtcttca aaagaagcac aataagtatt ttctgtagta
    11641 gttgcttgca ttccatctac ttttatacta ccagcataac aatatccact agtattataa
    11701 gcacaattag tagcagcaca atttaaatta ttatttttca taaataacct cctaagtttt
    11761 atttaaactt attatgtgaa gttatctaaa ttttatacac ttatttacaa ttacatttat
    11821 cacatttaac aactacatct ttggaagttt cccttcctaa atttttatta acatgataca
    11881 catcaacatt ttcagttgat ccacatatag aacatttaaa attgttttct agtagggttc
    11941 tttctttaaa taacttccaa tacatagaat tgatgtaatc attataattt accttgaggc
    12001 atgtctcaag aaagtttatg tatagattgt ctaaatctcc tgtatttaat tctgtatatt
    12061 ctggttcaaa atattcacaa catcctctaa aatcgggctt tgtatctgaa ataacacatc
    12121 taatatcata tctatagtct aggctaggcc ccttaaatga aagatgtgtg caaaaagaac
    12181 aagatatttc tggtagattt ttttcaagtt catgtaattt attattcata agtattcctc
    12241 caaaaaattt attttaagta tataaatgaa tagattattt taaaactttt atttatttat
    12301 attagagatt taggataaat attccaataa ggagtaaata cttaagtatt tattactgta
    12361 ttaaataaaa aagaggcaaa tctaacttga gattgcctac ttttttatca ttttttaata
    12421 aaatttattc taagtattat tttatttaga tttagtcagc caacttagta tctgctttgc
    12481 tttttaactt tctaccagag cgtacagtta aaatagcata taatacagta aatatggcag
    12541 ctaaaacata tgatatattt atgtttaagt taaatccaat ttgagcatta aatatataac
    12601 tgaatataac aaaggaataa aacataccag gaattaagct tataatataa ttcttttctt
    12661 ttattataag gtaaactgta atcattgcaa atgcaaatat ggcaatagtt tgattactcc
    12721 aagagaagta tctccacaat atattaaatc cagaagcact aagctttgca aatataagga
    12781 tggctatgac aggaataaat atacagattg acaatataac acgatgtttc ttttcctttt
    12841 ggtcataatg aaagtaatct gcaagcatta atcttaaaga tcttaaagct gtatctccag
    12901 aagttattgg taaaacaatt acacctatta tagctatgat accaccaata gaaccaagta
    12961 aatctcttgc aaccaaacct attacatcag gagaacctac taattcttta ggtattcctt
    13021 tattgtaaat acccatagca gctgcagccc aaatcattgc aattaaacct tctaaaatca
    13081 tcatattata aaaagtagtt tttccttctt tttcatttga aactgatctt gctataagag
    13141 tagcttgagt tgagtggaat ccagaaacaa taccacaagc aactgtaaca aagaatgtag
    13201 gaataagagg aataccatca ggatgaatac ctttccaatt tgcaagactt aaattagcta
    13261 aatcataacc ttgtgtaaag attccaattc caacaccagc agcagataaa agtagaatag
    13321 ctccaaatat tggatatacc tttcctatga ttttatcaat tggaaatagt gttgcacaca
    13381 agtaatatag aaagataagt ccataaacta tccagacaac tggattattt atagtcgatt
    13441 tcatatttaa aatttgagta acaactaaat ccccaggtgt ataaataaaa actgcaccaa
    13501 ctaaaagcat taaaagacaa aggaatatat tatagacttg aaatacttta ttacctaaaa
    13561 atctactgat aagagaaggc atttgtgctc ctttttctct tatactaatc attccactca
    13621 tatagtcatg caaagctcct ccaaatacac agccgatagg tattagtatg aatgctattg
    13681 gaccaaacaa tataccttgg ataggtccta gaattggtcc tgtaccagct atattaagca
    13741 actcaattag actatttttc cattttttca ttgggacata gtctactcca tcactttgag
    13801 caagtgcagg tgtttttctg tcatctgggc caaatacttt ttcacaataa gcaccatata
    13861 aagcacctcc tattagtaat atagccaagc cgattaagaa tgttgtcata atatcacccc
    13921 atttataaaa ttttttggtc tttgtataca tattatctaa atgtgtagat ttatattgaa
    13981 tttaattctg gacaacctac aaattctata aagtattaca actaatataa tttcagaaat
    14041 aaatacatat acataaccat gtacttagta tacaggtatt tggtaatatt ttcaatgtta
    14101 tagcaataaa gaatataaac aaaattctga taatatttta caaaaaaatt aaaaaatata
    14161 aaaattagtt taaatagatg gagaaaaaag gaaaatcaat tgagaatata aatgtctata
    14221 tcttaacaat attaagaggt ttaaaattct tgaagaaaga atagtcaagt gatataatgg
    14281 gtttacaggt gtatatacac tagtacatat agaaagcggt gcaattatgg ataaagattt
    14341 aacatatcaa atcaaagagc taaagaaaga aaaaaatgca ataatacttg cccattttta
    14401 tcaaccacca gaaatacaag aattagcaga tgcagttggt gactcatatt atttaagtga
    14461 gattgctaga gattgtaaag aaggagtggt tgtattttgt ggagtaaggt ttatgggaga
    14521 aagtgctaag atattatcac ctgaaaaaac tgtattgatg ccagtaagta acgctgggtg
    14581 tgctatggca gatatggtag atgaagaagg cgtgataaaa ttaaagcaac agtatccaaa
    14641 tgctttggtt gtatgttata taaactctac agctaaagtt aaggcacatt gtgatgtttc
    14701 tgttacatct tctagtgcaa ttaaaatact agaaaacata gataataaag aaattatatt
    14761 ccttccagat aaaaatttag gaggatatat tgcagaacaa tttccagata aaaactttat
    14821 attttgggac ggatattgta aataccataa caatataaga gctgaagaaa taattgagtt
    14881 aaaagataag tataaaaatg cagaagtatt agtacatcca gaatgtaaaa aagaaataag
    14941 agatttagga gattatgtag gtagtacatc aggaataata aaatatgcta ctaatagtaa
    15001 aaacaaagac tttataatag caactgaaga aggtatatta catgaattaa agaaaaataa
    15061 tcctaataaa aacttttata ttcctggtgg aaaaatacta tgtacagata tgaaaaagac
    15121 aacattagaa aatttatata gtacacttaa aaatatggaa aatgaagtta ttgtagaaga
    15181 tgaaatcatg gaaaaggctt taaattcact gcttaacatg cacaaattag cagaaggata
    15241 gagggataga cttatgaatt tagaacaaga tgttttaata gttggaagtg gagtttcagg
    15301 attatactgt gcattaaatt tagataaaag tttaaatgtg ctagtagtat cgaaatcaac
    15361 aattgaaaac aacaatacat atcttgcaca aggtggaatt tcaactgcta gaaatattga
    15421 tgatatagaa tcctttgtag aagatacaat gaaggctggg cagtataaaa atagagttga
    15481 agcagttcaa gtacttgcag atgaatctat agaaaatgta ggtcagatag tagaatatgg
    15541 tatgccttta gacaaagaaa atggagagat agattacaca agagaaggag cacatagtgt
    15601 aaatcgaata gttcatagta aggataatac tggagaagtt gtttttaaaa ctttattaaa
    15661 agaagctaaa actagagaaa atataactct tatagaagat gcatatcttt tagatatatt
    15721 aaaagatggc aataaatgta taggagctag aatttttaaa tctaaaaaag agattcatgt
    15781 tttttcaaaa atagtagttt tagctacagg tggaattggt gggcttttta aaaattctac
    15841 taatcaaaga catcttacag gtgatggaat tgcaatagct ttaagaaata atataaaaat
    15901 agaaaattta gaatatattc aaatacatcc tacagccttt tatgaagaaa ataatgaagg
    15961 taggagaatg cttatatctg aatcattaag aggagaggga gctaaacttt taaataaaaa
    16021 taaagaaaga tttgtggatg aacttcttcc tagagatgta gtttcaaaag ctatttttga
    16081 gcaaatggaa aaagataaat taccttatgt gtatttagat gcaacacatt tagatagtga
    16141 atatttaatt aatagatttt cttttattta taatgaatgt ttagctagag gaacagacat
    16201 aacgaaagaa tgtataaagg tatctcctgc acaacattat tttatgggcg ggatacatgt
    16261 agatttggat tcaaaaacat ctatggaaaa tttatatgca gttggtgaaa ttagttgtac
    16321 aggtgttcat ggagccaata gattggcaag taattcatta cttgaagggc ttgtattttc
    16381 aaaaagagca gctaaaaata taaactcagt tatagataat gtaaaagtta agtttataga
    16441 tgtaccagat atggatatag atatagaaca ggtaaaaaaa gaaaataaaa tattagtcat
    16501 aaaagaaatt gaaagaacaa gtgaggattt tggcgatgaa ttatttgatt attgatagaa
    16561 tgatacagga tgctttgata gaagatgttc caagtgaaga tataacaact aattctattg
    16621 tggatgaaaa ttctaaatct actgtagatt taatttgcaa acaagatggt ataatagctg
    16681 gcttaggtgt atttaagaga gtttttgaga tacttggtga tgtagatgta aaattatata
    16741 aaaatgatgg tgatagagta aagaatcgtg aaaagatagc tgtattaaca ggtagtacta
    16801 gaaacttact tgtaggagag cgtgtagcat tgaattattt acagagaatg agtggtatag
    16861 ctactattac aagtaggtat gttgaaaagc ttgaaggtac aaatacaaaa ttattagata
    16921 gtagaaaaac gatacctaat cttagaatat tagataagta ttctgtcaaa gtaggtggag
    16981 gatgcaatca tagatttaat ttatctgatg gaatattgct taaagacaac catattgggg
    17041 ctgctggtag tgttaagaag gcagttgaat tagctagaaa aaatacttct tttgttagaa
    17101 agatagaagt ggaagtggaa actttagaca tggtaaaaga agcaatagaa gccaatgcag
    17161 atataataat gttggataat atgagtttgg aaatggcaaa acaagcagta gatattataa
    17221 atggaagagc cattgtagaa ttttccggaa atgtaaattt agatactata gaagatatag
    17281 gaaaaattgg tgtggatgtt gtttctgtag gagcgcttac acattctgtt aaagctctcg
    17341 atataagtat gaagaatttg agaaatatat aaattttata ttgaaa
//
LOCUS       DAEMGG010000083        17289 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEMGG010000083 DAEMGG010000000
VERSION     DAEMGG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.083"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            34..780
                     /locus_tag="KPK24_002520"
     CDS             34..780
                     /locus_tag="KPK24_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG2300187.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            841..1515
                     /locus_tag="KPK24_002521"
     CDS             841..1515
                     /locus_tag="KPK24_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG2300188.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            1884..3230
                     /locus_tag="KPK24_002522"
     CDS             1884..3230
                     /locus_tag="KPK24_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG2300189.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3257..4657
                     /locus_tag="KPK24_002523"
     CDS             3257..4657
                     /locus_tag="KPK24_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300190.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYKNLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4867..6303
                     /locus_tag="KPK24_002524"
     CDS             4867..6303
                     /locus_tag="KPK24_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG2300191.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            6647..7591
                     /locus_tag="KPK24_002525"
     CDS             6647..7591
                     /locus_tag="KPK24_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG2300192.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7760..8692
                     /locus_tag="KPK24_002526"
     CDS             7760..8692
                     /locus_tag="KPK24_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG2300193.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENTLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8797..9264
                     /locus_tag="KPK24_002527"
     CDS             8797..9264
                     /locus_tag="KPK24_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG2300194.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9508..10362)
                     /locus_tag="KPK24_002528"
     CDS             complement(9508..10362)
                     /locus_tag="KPK24_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBG2300195.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10528..12492
                     /locus_tag="KPK24_002529"
     CDS             10528..12492
                     /locus_tag="KPK24_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2300196.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12492..12953
                     /locus_tag="KPK24_002530"
     CDS             12492..12953
                     /locus_tag="KPK24_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2300197.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12956..13276
                     /locus_tag="KPK24_002531"
     CDS             12956..13276
                     /locus_tag="KPK24_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG2300198.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13292..14392
                     /locus_tag="KPK24_002532"
     CDS             13292..14392
                     /locus_tag="KPK24_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG2300199.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14512..17112
                     /locus_tag="KPK24_002533"
     CDS             14512..17112
                     /locus_tag="KPK24_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG2300200.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDENIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYHIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6869 a         1779 c         2996 g         5645 t
ORIGIN      
        1 gaaaaatgta aactgtgaaa tggaggtagt aacatgaaaa ttgaaaatct ttctattgat
       61 gatattaaac gaggctatat atttaatgat gatttaaatt gttatagttg cattaaatgt
      121 acaaaaacat atgaggtagg agaaatatat aaaataggta atagattctt tgaagcttca
      181 aaagctattg aacttcatat ggaattagaa catagtgatt atttagagca attgcttaat
      241 agtgattcaa aatacaacac attgactgaa aatcaaaaac aattatttca attatttatt
      301 agtgatttac cagataagga gatagcaaaa caggttggag tatccacatc tactatacgc
      361 catcaaaaat ttattttcag agaaaaagca aaacaggcta agctctattt agcactatat
      421 gaatctgtat ttgaagataa atctaataaa agtagtacta taataccaat ccatgaacat
      481 gctcgaatgg tagatgaaag atatgtagta actgaagaag agagaaaaca cattttggat
      541 acatcatttg aaagtatgaa tcctttaaaa ttaaaagtat tttcttcaaa agaaaagaaa
      601 aaggtcgtaa ttttatctaa gattttggaa caatttgaac gtgggaagaa atattcagaa
      661 aaggaagtta atcagatttt agaatctata tatgatgatt ttgcaacaat tagaagatat
      721 ctaattgagt atggttttat gagtagaaat aaagaatgta caaaatattg gttaacataa
      781 ttaaaataat aaattttact aatgtgtaat gttattttta tctaggggga ggagtcaaat
      841 atgaatgatg aattaaaatc tattttatac aataaaggtg tagacattgt tcgctttgtt
      901 gatatatctg aatttccaat aaatcaaaca caaggttttt ctaaagcaat tttattttgt
      961 atagggttat caaaaaaatt tattacagat atatacaata acttacctac agatagtgat
     1021 gagtttttgg aaaaagagga gaaagtagaa gaattagctg actggatttc aaaatatatt
     1081 caaaataaag gatatagagc atattctcaa tcggagaaaa acaatctaga acatggatat
     1141 tttgaaaaag cctatattaa tccagaaatg caatcaggga ttagtccttt accacataag
     1201 acaatagcaa atattagtgg cattggattt atgggaaaaa ataatttgtt tgtaacagag
     1261 gaatatggat gtgcttttag catgtgtaca gtacttacag atgctccaat ttctgttgaa
     1321 agatatcctt tgattgattc aaaatgtatg gattgcaatg tttgtgtaga gaactgtcct
     1381 gcaaaagcta tacatggaaa tgaatggact ttacctggaa aaagagaatc tattattgat
     1441 gtttcaaagt gtttttgtgt attgaggtgt atgatgagtt gcccatggtc attaagatat
     1501 gcaaatcaaa aataacttta ctagagaagg tattaaattg aatattataa aatttttaaa
     1561 atgtaagatt atataagatt tctcaaaata aaaatttgta tagaacactc agaaatgagt
     1621 gtttttttat tgggattaaa ttatattgat acaaagctta actattttaa tcattatata
     1681 aaatttgtat taaaaaatta agaataactt ggtaatagtg aaaatataat gagattataa
     1741 ttgatttata atataactat aatgatattg taaaaatgaa aattgtcctc tcaaaattcc
     1801 caaagagagt aaaagtcact tcagttatag tcaaaaaaat ttttctgtga aaagaattta
     1861 gatttgaaaa ggaggaatta atcatggaaa ttgaaactaa aactccagat tggattaatg
     1921 tactagaatt aaacagtaaa atagatatta ctgggaaata tgatgtaagt aatatgatac
     1981 aaaatattgt ctctgataag tcactagaag gttccattat atattttcca aaaggtaatt
     2041 acttatttga gaaaggtatt aaaattagtc aacaaataac acttcaagga gattcttatt
     2101 atggaggagg taatcaagta tcaaatttag ataaaaaaca atcaatcata ggaacaacca
     2161 attttataac ccgaggagtt tctaatatga gtattattac attgtcagga acaagtcaat
     2221 gtatcaaaaa tattaatttt tattatgata gtcatgatat agagaaaata ccgcctaaaa
     2281 atgtatctgc tattacagaa tatggagaaa cacaaggact atctcatttt gaacatctgt
     2341 ttatttctgg attttcaggg ataggaattg aaattccata ctattcaact ggtaatgata
     2401 ttactgttag ttcatgtggt cttggtatga ggcttggaga gaagtcaatg ctttctagta
     2461 gcaagattta cgaatgtaaa aatggaatgg agattactac tggtgttagc ttaaataatg
     2521 tgagaataga agaaataaaa gaaataggta ttaataataa aggatttggt tttaacctga
     2581 ttatgaatct tatggtcgac caatgtggat attgtggatt tatgtttgaa aaaatgtgct
     2641 atagccagat aactgccaga attactagat gcggtcagta ttataaaagt gttgattata
     2701 atgcttatga gaatatggaa gataggaaag aagaagctta ttccattttt tatggagata
     2761 ttttagaaaa ttgcaatatt gtattgatga atagtaatat tgataccttg gaaaatgatt
     2821 cacttgatga acataagata tatgtaatca aggcaaatga aacaaacaat gtaatgctaa
     2881 catgttatgc tgaagcagat aactttatac aatgtgcaaa gggcaacttg ctgttagaaa
     2941 atggtgaaaa tacttataaa ttttataatg gtgagatttg ttctgtagga ggtgttcaaa
     3001 tttctgatat agatgaaaaa gatttaatca aaataataga gaacactttg tatatagatg
     3061 ataaaggtaa gtatttaatg gttcctagat taaaaaagaa tactgtaata tattcagtac
     3121 tagattctac agaagaaata aataaattac atggtggaat atgggaacag attgatgtaa
     3181 aagtattagc tggggaatcc atgtactatt ataaaaagat aaaagattaa attatcaaac
     3241 taggaggtag tgattaatgg aagagtctat aataattaaa gatataagtg gctggattaa
     3301 tgtccttgat tatggaattt gtaatgatga aacaaaagat gtaagtttta agttgcaaga
     3361 gattatggat gctgtaccag aagattctat tatatatttt ccaaaaggaa aatacttatt
     3421 tgaaagtggt gttaagatta agaaacgatt aactttatgt ggagattctt atattttggc
     3481 gaatgttcct aaaaatactg ctggagtaac acaatttact tttaaaggaa agaaaaatcc
     3541 taaaagtgaa aatatagcaa ttataacagc aaaaagtgta agacactgta ttaagagcat
     3601 ttgcttttat tcagatagtt gtgatagaga tatgacatct aatcatgaac ctccaacaga
     3661 tggaaaacct agatttcatc ataatgtaat tataaattat agaaatatat cggctattgt
     3721 atgtgaagat aaacagggag aatcaggtca ttataaaaat ctatctttta gtggtttttc
     3781 tggaattgca cttgatatgc ctaataattc tacagcaaat gatattacaa tttttacatc
     3841 cggacttgga attagtacag gagaaaattc aactattgct aacagtaagg tttggggctg
     3901 tgttgatgga atgataattt ctactggaac ttttataaat ggtatgaggg tagaagaaat
     3961 aaaaaaagtt ggaattaaaa atattggtag aggtttaaat tttattacaa atatcacaat
     4021 tgaccaatgt ggatattgtg gagtttggtt tgattctata tccaatgttc agatatcagg
     4081 aaatatcaca aggtgtgggc agtactatta tgatgtaagc tatgataatt atttaatgtt
     4141 ggataataga gttaaagaag catattcttt attctatggt aatttattgg aatcaagtaa
     4201 tatagtttta tcaaatacca ataaagataa ttgggaagat ttcaaagaag ataagcataa
     4261 ggtgtatctt gtagaagcac ttgaaaccaa gaatgtacta ttaacatgca atatagatgc
     4321 tactgactgg attgttaagt cagaaaaagg aaatttaacg tacaataata tgcgacaaac
     4381 tgttatattt tacgatggaa aactaagtag tatcaatgga attggtatat ctgatgaaga
     4441 ttatggggat aaagtcaaga taaaaaaagg aaatatatat ataaatagta aggataagga
     4501 tattaagatg cataaaccag aggtaggtga cattatgtca acaattatta aagataaaag
     4561 tattctttct gctcattatg ggggaacatg ggagaaaatt ggtgaaaaaa tagagtttgg
     4621 aataactgta cactattata aattgataga gaaataagta aatacgtagt atttgaatag
     4681 atgtatttta ttggagagct aggactatag ttttaatgta aatggtttgg taatagcatg
     4741 gattgaataa tataaaaata atataaaaaa ttaatttgat tatgtaaaat tctatgaagt
     4801 agtaaaactg tgactttagt cataggaggt tcaaactaag taaaagggaa aggaatatat
     4861 aatattatgg aaggaagaac tgggattaaa gatggaagat ggattaatgt agtagaaaga
     4921 ggtattaaaa atgatggttc gtacaacgta ggtcctgctt tgcaggatat tattgatgaa
     4981 atagaaacag atttttgtgt cttatatttt ccaaaaggta aatacctatt tacaagtggt
     5041 gttaaaatta ataaagaaat tgtattgcaa ggtgattcta attcatctac aggcactaca
     5101 caatttataa ctcgtggagt agaaaatatg agtattatta cattgacagg gaaaaaacaa
     5161 tgtattaaaa atataaattt ttattctgat agttgtgaga tacaggtaaa taaagaacca
     5221 ccgacaaaag gtaatcctaa atatcattat gagatgatta ttaataagaa tgaaaagaat
     5281 gttgagatga ataatgtatc agcaatcctg tatgataaca gtaagaatat gattaaaggt
     5341 ctaggtcatt atgaaaatat ttatataagt ggtttttcgg gtacaggaat taggatacca
     5401 tattactcaa ttgtaaatga tattacagtt tcatcttgtg ggctgggtat tgatacagga
     5461 atagacacaa ttatttctaa tagtagaata tctaagtgtc aaaatggaat gaggattaga
     5521 actggaacta gtataaataa tgtaaggatt gaaaaaatac agaaagttgg tcttataagt
     5581 agtcaagtta aggggtatga ggggtatgga agttataaaa ttaataatat tacgattgac
     5641 caatgtgggt attgtggctt ttcatttgat tcaatgaaag atagttattt ttctggtatt
     5701 ataagaaggt gtggtcagta ttattataat acagattatg acacatattt aagtattaaa
     5761 gatagagtag aagaagcata ttctttattt tatggaaatt attttacaga atgtaatatt
     5821 gatttagtta gtgataataa ggataactgg gatgatggct tagaatataa acataaagtt
     5881 tatgtattta aaacccttca aataccaaaa ttaacattga gatgcaatat agatgcctat
     5941 gattatatcg ttaaatcggt acatggagga ggtaatttaa tgttaaaaaa taattttaac
     6001 aattatatat ttattgattc atctaatcca gaagttatta atggaattaa ttttaattat
     6061 caaaagaatg gaaacttgtt aaggttaaat aatggtaaat taagttataa aaatggaaag
     6121 gaagaaacag ctatattcag acctaattat ggtcttattg tagcatcaac agattcagaa
     6181 tgtgaacaaa tagagactag ctatggagga aaatgggaga agcttggttc tgaagtaatt
     6241 ggagaagaaa cggtatatta ttataaacat catgaaccag ataaaacaaa ccaaatacaa
     6301 taaaatttgt attatataat gatataaagt tttaatatat agtgtttacc tttaatgtaa
     6361 tattggtata tttgtatagt attaaatttg ttaaaaagta aaattgtagg gtgcttgaaa
     6421 atagcacctt ttttagtttc attataaaat tagttttgaa agtttaggaa taatgtatat
     6481 ttatttttaa agaattataa ccttattaag gatattttaa atgctagaaa tgaatcagta
     6541 aatcatactg gaattatctt aaatgttttg attaaattaa gtatttagag ctataattat
     6601 attaaaaata tattagaaca taattatatt actatggagg ttaaatatga tagtaagtgt
     6661 gagtagaaga actgatatac ctgctttcta tagtaagtgg ttttttaaca gacttaaaga
     6721 aggttttgtt tatgtaatta atccaatgaa tccaaaacag gttagtaaga tagagttaaa
     6781 tccacatact gttgattgtt ttgtattttg gacaaaggat gtaactccaa tgttatatga
     6841 tttaaataaa ttaaaagact ataaatatta ttttcactat actataacat catatggaaa
     6901 agaagtagag acatgtattt tagataaaag aaaggtaata gattcattta aggaattgtc
     6961 tagaaaaata ggcaaagaaa gagtaatttt aagatacgac ccgattttct taagtgaaaa
     7021 gtacacagta gattatcata tcaaagcatt tgcgagttta tgtaatcagc ttgatggatt
     7081 tacagaaaaa tgtataataa gttttatcga tttatataag aaaacaaaat ttaatactaa
     7141 agcattacat ataaagccaa tagaaattac agaaatagaa acattatcaa aagagatttc
     7201 taagattgct cataagcatg ggataacact tgaaatgtgt tctgaagaat ataatttaag
     7261 taaatttggg attggaaaaa gtaagtgtat tgatgataga ttaatatcaa aaattatagg
     7321 atgtgaagta gaggtaaaaa aagatagtac acaaagagat atttgtggat gtgttaaaag
     7381 tgtcgatata ggacaataca atacttgcag acattattgt ctgtattgtt atgctaactt
     7441 taattatgca aaagtagaag aaaactgtag gctttataaa gaagataata aattactcat
     7501 agatacttta agagatgatg caaaaataag cattagagat atgaagacta ttaaagtaga
     7561 taagaataaa caaattagta tatttgatta aaataaatat actaattacc ttaatatatt
     7621 aagaatatag atacttttga taacaaaatt aaacttaata gatattttct atataagaaa
     7681 gatggtcata atatgtcaat ttgaccatct ttttgatata attaaaacac aaataattta
     7741 gtaacaaagg aggcagaact tgaatttatt aaaaaaatat cctataccta ttgcaagttt
     7801 gattttgtca atctttactt taggtaactt attacaatct tatagtgaat cattacgtaa
     7861 tgtaattgga tttataggat ttattttgta tgcaatttat atagtcaaaa taatattact
     7921 tagaaagggt gttaaagagc aattagagaa cactttaata tcaagtacat ttccaactat
     7981 aacaatggca acaatgatat ttgctacata tataaaacca ttatctttag aattaagtat
     8041 tatgatttgg agcataggaa ttgtgggtca tcttgtgtta atagtcttat ttagtaagaa
     8101 gttcttagtt aaattctcaa taaaaacggt atttccatct tggtatatag tatatgttgg
     8161 gatagttgca gctagtgtta cggcaccagc atttaatcaa cttttgattg gcaaaattgc
     8221 tttttggata ggatttgtat cttatatagt tcttatacca gtaattttaa agagagtatg
     8281 gataatcaag gaaatgcctg aaccatcttt accaagtata gctatattag ctgcaccagg
     8341 gtcactatta ttgtctggat atattaattc tttttcagat aaaaataatg tagtattata
     8401 cttgttattc atattatcaa ttgtatttta tattatagtt ttgggatatt taccgaaatt
     8461 attaagattg cctttttatc cgagttttgc tgcatttaca tttcctatgg caataagtgc
     8521 aatgggtatg aaactaatga atggttattt aactaaatca ggtcatttta taagttggat
     8581 ttcatatgct tctaagttag aagagataat tgctacagtg attattttat atgttttagt
     8641 tttgtttatt atatatctta gtaaatctga aactacatct aaagctaaat aagaattatt
     8701 aaatatgtgt atttaatagg aatattgata taaatatatg aagataagat aaaccataga
     8761 aacttaattt agcttaattt tattggagga aaacaaatga aaacaaaaga agaatattta
     8821 aaagaagtta gaaacatatc agaagaatac tttagaaaag gagagttttt ttgctctgaa
     8881 gcagttctcc agacaataaa tgatgcttta ggccaacctt taagtccaga gataactaag
     8941 ttagcatcag gatttccgat aggacttgga aaggcacaat gtctttgtgg agcaatatct
     9001 gggggagaga tggctttagg tatagtatat ggaagagttc atggtgaagc tatgaatcca
     9061 aagatgtttg agcatgctaa aggtttgcat gattatatta aaaaagagta tggtgcaaca
     9121 tgttgtagag ctattacaaa gaaatgggat ggtgataact ttatgtctcc cgaaagaaaa
     9181 gagcactgta ttaaaataac aggtcaagtt acagagtggg ttgcagataa gcttatagaa
     9241 gatgaaaaac ttaatatcaa atagtgtaat attagttggt gaatttataa agttaaattt
     9301 acttatagta taagatactt gatattctcg tattgataga aaaatttata gcatatgtat
     9361 ttaatgaaat atacttatat ttagagtgtg gtttaatttc ataaatacat atgctattat
     9421 tttaagtata taagcaaatt tatttaaata aatagaaaca gttaatttat atgtacaaaa
     9481 ctatatatga ttagtgtaaa taaataccta ttttataata ctcaacataa agacaccaga
     9541 catgattagt attataccaa taaaaatagc aagccattct tttttgttct tcttttcttt
     9601 aaaaaatata atccctccta aagtggctac tatcacacaa ctctgagaga tagaaaaact
     9661 tattgtaact cccaatgtat tagtcgttat gaacatacct aaatttgcta ttgaccacaa
     9721 tataccagta attaaattga ataaaacatt ttccctatat acaatctctt tagaaaaata
     9781 cttgacaata atagatgaag ttatcaacat accaacagat tgaggtaata taattctata
     9841 tccttcaatt aaaaatattt gattagttac aacatataat gataaaaata gagatgataa
     9901 taatacaact ccaacacatt ttaacatatt tttatctgta gattttttag tttgataatt
     9961 tgtgagaagt ataccaagta ttatacataa tattgataaa gaaccaatca gtaacatttt
    10021 agaactggtc cattcattaa acaagattac agctaataat gtcgtaaaag ttaattgtga
    10081 accatttgaa attggcatta ctttagatac actagcaatt tgaattgctt taaactgcat
    10141 taattgtccc aaactccaga atatgccaga aatcatactt atacaaaatg aaaaaaatgt
    10201 aatttcagga tgtataacca gagttattat agtggaaaat ataaacgcac ttatggatgt
    10261 acctaaaagc tgttcatgag gagaactttt ctttagattt gcaactatgg gcatcaatcc
    10321 ccatcccatt acaggaagta aagcaataat tattgtcagc attaaactat ttcctttcat
    10381 attaaattta gacagctttt aatttatcat ctaaaattaa agtgttcaat acataaaaat
    10441 taccgtattt aatcaggaaa tattagtagt tttttagaaa aacattttcc attaaaataa
    10501 agacaaaggg aaggagtaaa attgaaaatg ttaactgata tgattcaaaa tcgtgaaatt
    10561 aaaataataa agtttttatt aaaagaaggt catacaactg taaaagcaat agcagatgat
    10621 ttagatgttt cagaaaaaac agtttcaaaa tctcttaaag aagtagaagc agtgcttaaa
    10681 aagataggtc tttctttaat aagaaaacct aagataggtg tttatattga tggagattta
    10741 aaaaaaatac ttccttacct agataataaa ggaagtcaaa ttcctacaac cagagaggag
    10801 agagttatct atatatattc agtactttta aagagtaatg attatattac tatacaacaa
    10861 ctttctgatg aactatatat aagtcgtgga actattgaaa gggatttagc agaagttgaa
    10921 aaactgctaa aaaacgaagg gatttccata tataagaaac ctagtaaggg aataaagtta
    10981 aatataagtg aaagagataa aagacaactt actgctaagt ttatacataa attttggagt
    11041 agaaactggt atattaagca agagcaagaa agcttttatc aagcatttga aaagatacaa
    11101 gcagatgtaa atggtatttt ttcggaagat gatttatcaa taattattga tattcttagg
    11161 aatttcagca gagaaagaaa atttgagttc tctgattatg aatttcagtc attagttatt
    11221 catttggcaa tagctgtaga gagaattaga aaaggcgaat atatagaaga aagtttgtat
    11281 actggtaata tagaaaataa tcaaatgtca aataccaatt atttagcaaa acaattagaa
    11341 gaaaaatttt caatattatt accaaaatct gaaattggat atataaattt acatttagta
    11401 gcgtctgacc aacatagaga tactaatgaa ctaaaaaata cttcaactaa tccttgtgag
    11461 acaagtacaa ataatatatc aaatcttcgg gaaattataa aaaatacatt gtatgagaca
    11521 gcttatgaca aggatttaat tgatggattg acttcacatg tacattcatc aataaataga
    11581 ctgaaatatg gacttagtat taaaaatcca tatgtaaata caatcaaaca aaacttttct
    11641 ttatcatttg agcaagcttt agatttaaag aaagtaattg aatttatata tgatattagg
    11701 ataaatgatg atgaatgtgc ttatatagca ttacattttg aagcttttag agaaagaatg
    11761 aaagaacttc cacaaaatat tagagtaatt ctagtatgca gtacaggatt aggttcatct
    11821 aggttattgt ctgcaagaat aaaaaaatac tttccaatga taaagattga aaaagtactt
    11881 tccgtacaag aattagtatc cagtaaaatt gatgcagatt tagtaataag tactatttat
    11941 ttagagttag aaacaattcc aaccatagta gttagtccta ttttaaatga aagtgatata
    12001 agaaccttgg aaagaaatat acaaaatgtt tcaagttgta cttctaagaa gtataaatct
    12061 ttcagaaact taatctttga agaaaatata ttgttgaata ttgaagcttc aaactatgaa
    12121 gatgcaatta agtatataac agataaccta ataaaaaaag gttttgcaaa agaaggtata
    12181 gctaaaagtg caattgagag agaaaattta tcttacactt ctttccaatc aatagctaca
    12241 cctcatgcta atcctagtta tattaaaaaa tctaatatat ctgttgcaac gttaaaaaat
    12301 cctattacat ggggaaatga aaaggtggaa gttattttct ttatttcatt agaaaatgac
    12361 aaaaacctag agcttgatga aatatatgaa gttttttatg actttattga caataaaaaa
    12421 aatataaatg acatattaag tgcaacttcc acaaaggaag tgtacgattt gctagtggag
    12481 gagagtatat agtgaaaata tcagatgtat tgaaaaaaga gcaaattgtt tttaatcttg
    12541 atacaaatac aaaggaagaa acaataaaaa aattaagtag tatatttgtt gaaaatggga
    12601 ttgtggacaa tgaagaagaa tatgttaagt ctgttttgga aagagaagaa cattcaacta
    12661 caggtattgg taatggtatt gctattcctc atgggaaaag taatgctgtc aaaaaatctt
    12721 ctataatatt tgcaaagact aagcataaga ttgaatggga tgctttagat gaaaaaccag
    12781 tagatagtat tttcttattg gctataagca atattgataa ggatagcaat catcttgtac
    12841 tattatctaa attagcaact aaacttatgg atgatgacaa tgtagatgca ctaaaattag
    12901 ccaattcaga acaagaaata atcaatatat ttagtgaaga aggagagatg tagaaatgaa
    12961 aagaaaaatt atagcagtta cagcatgtgc aacaggagtt gcacatacat atatggcagc
    13021 tcaagcatta aagaaagctt caaaaaagat gggtcacctt ataaaagttg aaacacaagg
    13081 agctacagga atcgataatg agttaactac aaaggatgca caaattgcag aagtagtaat
    13141 atttgcagta gatactaaag tcagaaatgc tgaacgtttt gagggaaaag aaatactaga
    13201 agtaccagtt aatgcaccta taaaggatgc agagaaagta ataaatgatg cattaaagtt
    13261 aattgacact aaataaatgg agggaaatgt aatgttgaaa aagatagtct ctgagttaaa
    13321 aaagcatgtc ttaactggaa tctcatatat gattccatta gtaattgcag gtgctatgat
    13381 aatggcgata tctcgtgtag gtggttcttt ctataatata ccagatatat gggatgctaa
    13441 atatgcagaa agtgctagta gtattgttag attattacat gatttagatg ggtttggagg
    13501 tactgcatta ggattaatgt ttcctgtaat tgctgcattt ataggatttt ctatagctga
    13561 taaattagct attgtaccag gtcttgttgg aggtatgatt gctaaagata taggtgcagg
    13621 atttttagga gctttggcag ttggtcttat agcaggatat acatgtttgt ttattaaaaa
    13681 tcatgttaaa ttaccaaaat cagcagcttc aatagtacca atatttatag taccagtatt
    13741 tgggacatta atcactgttg tattaatcaa ttatgtgata ggtatacctt ttgccacatt
    13801 aaatactgga ttagaaaatt ggttaaatgg attatctggt acaaatcaga tactaatggc
    13861 agctattata ggggctatga tggcagttga cttaggtgga ccagttaata aagcagcttt
    13921 aacaacatca cttgcattat taacaagtgg aatttatgcg cctaacactg ctgcaatggt
    13981 aggaatagtt ataccaccac taggattggg acttgctact atactagcaa aacaaaaata
    14041 taataaacag ttacgtgaag ctggaaaatc atctcttata atgggtttaa ttggagtaag
    14101 tgaaggagca attccttttg cggtagaatc tccattaaaa gtaatacctt catgtgtaat
    14161 tggaacagca attgcttcag ctatggcagt tggtttaggt tcagtaaatg caactccaat
    14221 tagtggattt tatggatggt ttacagtaga gaattggcca atgtatgtgt tatcaatagc
    14281 agttggaaca gtaattgtag ctggattagt tatagtattg cgtggaaatc cagtgattga
    14341 ggatgatgaa tttgaagatg atgattttga tgaagcagag tgggaatctt aaaatttaat
    14401 aactatttaa gacgtgttat aagctatatt caatatgaat cttatactaa ataaagtgtt
    14461 aaatattcag tttagactta aatataaaag ataaagaaca gaggagaata aatgataaaa
    14521 gcacatattg ttaatcatac acactgggat agagaatggt attttacttc tggagatgca
    14581 ttagtactta gtgaacaact atttacagat gtaattgatg aattagaacg taatccagat
    14641 gtaagttttg tacttgatgg acaactttct attttggatg attatgtaca attacacaaa
    14701 gaaaaaatag aaatcattaa aaagttaata aaagaagata gactacatat tggaccatgg
    14761 tttactcaaa ctgatgcatt ctttgctaga ggagagtcta ttttaagaaa cttaatgatt
    14821 ggaatttttg aaagtaaaaa gtatggtaag tatatgaaaa ttgggtattt gccagataca
    14881 tttggattta atgcacagat gcctatacta ttaaagcatg taggtcttga taatatattc
    14941 ttttggagag gtatacatct tggtaaacag gttcaatcac catattttaa gtggaaaagc
    15001 ttaaatggag aaagttatat atatgcagta aatatgccac atggatatgg aactggtatg
    15061 cttttagagc cttcattaaa gtatgttaat ggacgcttag acccagctat tgattttatt
    15121 aaatcatata ctgatgtaga tgaggtgtta ataccttctg ggaatgacca gttaaatata
    15181 ataggtgatt tcaaaaataa aattaaagcg attaatgata tcggaaagta ttcatatata
    15241 acgagttcat atcaagagtt tttaaaatat ataaagtcca tagaatcact agaaagctat
    15301 agaggtgaat ttagagaacc tgtattagca agaatacata aatcaattgg ttctagtaga
    15361 atggatataa aattagcatg tgataaacta gaaaacaagc tgattaaaag aatagagcca
    15421 ttattagtaa ttgctaaaaa gagcaaaatt gaaataagca atcagttact tataaataca
    15481 tggaaaaaac ttttggaagg acaagcacac gatagtttag ctggatgtgt aactgatact
    15541 gtaacagatg atattcttca ccgtataaga gaagcaaatg aaatatgtga cagtattgaa
    15601 aatactattg taaaaaagat ttcagaaggg cttaaattat ctaaaaatga tattttagta
    15661 tttaatacag aagctaagcg ttttaatgga tataaagaga tacaagttgt aagtgatagt
    15721 aaaaatattt attttaaaga taatttagat gctactataa ttgaagaaac ttatgtaaaa
    15781 ccgagagaaa atgtattaga agaaacacct gctggtaata tatttattga agaaccagga
    15841 tattatatat taaaagtaag aataaatgta cagcttcctg cacttggtta taaagttgtt
    15901 agttttgaat taagtgataa agaaatggtg tcacttgata aatctaatga cacttttata
    15961 tctaatgata attataagat aatatatgaa gatggaagct taaatttaca acttaaagat
    16021 ggaacttaca tatcaaactt tttaacatta aaagatagtg gtaatgcagg tgatacctat
    16081 gacttttctc cactaaaaaa tgatgagaat ataaaacttt cgtttgataa agtgtataca
    16141 gaaaaagctt taggatatga aaaaatggtg gttaaaggta ctactttact accacttaca
    16201 ttagaagata gaaaaaacaa atatcttaat ggtaaattaa atgtaaaagt aagtattgtc
    16261 ttatctaaag aaaacccatt gatggatgta aaactttgtg ttgataacac gatatataat
    16321 catagactac gtgttcatat aaaaactgat ataaaagata ataaaaacat agcatcattg
    16381 ccatttggat atataacaag aaaaaatgga gttttagaca actgggaaga tatttacagt
    16441 gaaatgccta tagatttaga acctttggaa agtaatatta cgcttacaaa ccaaaataga
    16501 agttgtactg tatttactag aggaataaaa gagtatcaac atattgaaga tgaaattgca
    16561 ttgaccttac ttgctactac agatgaacta ggaaagcctg atttattata tagaccagga
    16621 cgtgcttctg gtgatacaac taaaaaaggt catataagaa ttaaaacaga taaagctcaa
    16681 atgcttaagg aattagaatt ttcatttgca atatatatgg attctaaatc atttgatgaa
    16741 ttggaaattg cgaatttaac ccacaattat ctaagggaat gtgtaaatta tcaattacag
    16801 gattacaatt tctttttata tcatatagat aataagattc aaaaatcaat tgtcaataaa
    16861 actgtaagta gagaattaga aataatatct ttaccagaaa actatcttat atcagcatgt
    16921 tatccttctt attacagtaa agataaattt ataataagag tggaaaatcc aagtagtaat
    16981 agaatgatat tggatgagca aatatttaaa aatagaaatg ggaaaattgt aaatgcaata
    17041 gaagatgtag aagaggaaca ggtatatgaa atagcacctt ttgacgttat ttcaatatta
    17101 ttggatttat aagtgtaaaa gtaagtttat tgtttaaagt ctgacagata ttaatctgcc
    17161 agacttttta atttgtataa atttgggtaa atattaatga atggttttag tatttaagta
    17221 tgaatttagt atttaatata gaatttattg aagtagaata attagtttaa caggataagc
    17281 agtttaaat
//
LOCUS       DAEMGG010000084        17281 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEMGG010000084 DAEMGG010000000
VERSION     DAEMGG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.084"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..2011
                     /locus_tag="KPK24_002534"
     CDS             134..2011
                     /locus_tag="KPK24_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2300201.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYIEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVISCKSSYVSTNDNIISLVLAG
                     N"
     gene            2366..3772
                     /locus_tag="KPK24_002535"
     CDS             2366..3772
                     /locus_tag="KPK24_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG2300202.1"
                     /translation="MNTGFPKEFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            3800..4648
                     /locus_tag="KPK24_002536"
     CDS             3800..4648
                     /locus_tag="KPK24_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2300203.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            5309..6001
                     /locus_tag="KPK24_002537"
     CDS             5309..6001
                     /locus_tag="KPK24_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300204.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            5998..6933
                     /locus_tag="KPK24_002538"
     CDS             5998..6933
                     /locus_tag="KPK24_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2300205.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPYDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDRDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTIFTVSLPLI"
     gene            7081..8004
                     /locus_tag="KPK24_002539"
     CDS             7081..8004
                     /locus_tag="KPK24_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300206.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            7988..8758
                     /locus_tag="KPK24_002540"
     CDS             7988..8758
                     /locus_tag="KPK24_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300207.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            8768..9550
                     /locus_tag="KPK24_002541"
     CDS             8768..9550
                     /locus_tag="KPK24_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300208.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSINLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            9927..10463
                     /locus_tag="KPK24_002542"
     CDS             9927..10463
                     /locus_tag="KPK24_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG2300209.1"
                     /translation="MNLKGTKTEKNLIAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            10495..10884
                     /locus_tag="KPK24_002543"
     CDS             10495..10884
                     /locus_tag="KPK24_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG2300210.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            10921..11307
                     /locus_tag="KPK24_002544"
     CDS             10921..11307
                     /locus_tag="KPK24_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG2300211.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPITKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            11434..12876
                     /locus_tag="KPK24_002545"
     CDS             11434..12876
                     /locus_tag="KPK24_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG2300212.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEVSNYTNIQHV"
     gene            13173..13988
                     /locus_tag="KPK24_002546"
     CDS             13173..13988
                     /locus_tag="KPK24_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2300213.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            14590..15774
                     /locus_tag="KPK24_002547"
     CDS             14590..15774
                     /locus_tag="KPK24_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300214.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(16151..17260)
                     /locus_tag="KPK24_002548"
     CDS             complement(16151..17260)
                     /locus_tag="KPK24_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2300215.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
BASE COUNT         6190 a         2142 c         3139 g         5810 t
ORIGIN      
        1 tgcaggcaag atcgaatcaa tttttataga ttatctatag agtactatgg gtataatcta
       61 tttatttggt tcgatttttt tgtttaattg gccataaaac aaaaagccta tagttgaaaa
      121 tggaggattt tttatggatt ataaaaatat agctcaagaa attctactaa atgtaggagg
      181 aaaagagaat gtaaatgaag tgactcattg tatgacaaga cttagattta aagtcaaaag
      241 cgcttctaaa gtgaataagg ataaattatc taaaactgaa ggagtaataa ctgttgttga
      301 gagtatgggt caaatacaag ttgtaattgg aaataaagta aaaaaagtat atgatgaggt
      361 cataaaaatt gtacctcaat caaataacat aggtaaaaag aatgaaagtc aagaaaagca
      421 aggtataatt aattctatat tatcagcagt agctggtata tttacaccta caatacctgc
      481 tatagcaggg gttggtatga ttaaaggtat tttatcagtt cttgctatgt attatatgaa
      541 taaaaatgga gtagatataa aagaaacgca aagctatatt atcttaaatg ctatggctga
      601 ttctatattc tattttatgc ctattatact aggatatact gcggctaaag tatttaatgc
      661 aaataaaata atatctatgg tattaggagc tacactttgt tatccaactt ttactgcttt
      721 gatggcaggt gaagaatctg ttagattttt aggattggca gtaacaaaag ctacgtatac
      781 atcatctgtt attccaataa ttattgctat atgggctctt tcatacattg aaaaagtatt
      841 agaaaagtat atacctgagg taattaaaat aataatggtt ccaactttat cattggttgt
      901 aatgttacct gcaacattat ttttatttgg accaatcgga atttatatag gaaatgtcat
      961 aaacttttct tataagtata tttatgaatt aagcccagca ttgtgtggag catttgtagg
     1021 tgggttatgg tgtgtgttgg ttatatttgg tgctcataga gcattattac caataggtat
     1081 aagtgatgtt gctcaaacag gaagacaaaa tttattggca tttgcaggag ctgcaaactt
     1141 ttcacaagca ggggctgctc taggagtatt ctttaaaact aaaaatcaag gactaaaaac
     1201 aatttctatg tctgcaacta taacagcttt atttggtata actgaacctg caatatacgg
     1261 tgccaatctt agattaaaaa aacctatggt atgtgccgta atatgtggtg catttggagg
     1321 tgcgataatg ggaatgggtg gagcttatgg taatgcattt gcaaaccaag gtgttttaac
     1381 tatacctgta tatgctgaag caggtgcatt aggtttctta agttatctag gtggttgtgc
     1441 tatagcattt tttggttcag ctatttcaac ttatctagtt ggatttgaag atttggaaga
     1501 gtcttctaat aaggaaaatt catctataaa agtagaaacg aaggatggtg ttattgatat
     1561 aacttcacct gtagagggcg aatgtataga attgtcagaa gtaaaggatg atgtatttgc
     1621 ttcaaaagct atgggagaag gtatagctgt gttacctaca aaaggagtaa taacagcccc
     1681 tactgattgt gaagtagctt ctttatttcc aacattacat gcaataggat taaaattaga
     1741 taatggagca gaaatgttaa tacatgttgg aatcaataca gtagaattaa atggtaaata
     1801 ctttacaaaa catgttaatc aaggagattt agtgaaaaaa ggtgataaac ttatatcgtt
     1861 tgatatagat aaaataaaaa aggctggtta tgatgtaact acgccagtta tagtaaacaa
     1921 tacatttgat tttggacaag taatttcttg taaatctagt tatgtttcaa ccaatgataa
     1981 tataatttca ttagtattag ctggtaatta agttaatttg actagagata agtaaattga
     2041 tttaaaagga aataagtaaa tcaacaatta aatacatcta aaagtaagtt tattgcaatg
     2101 aattgttaaa gaaaataatt aatatggagt aaatctattg caatgaagta acaaagaaaa
     2161 taattaatat agagtaagtt tattgtaatg aattgttaaa gaaaagagtt aatatagagt
     2221 aagtttattg taatgaattg ttaaagaaaa taattaatat agggtaaatc tatctcaatg
     2281 aaataacaaa gaaaagagtt aatatagagt gaacttattg caatgaatca acaaagaaaa
     2341 taattaataa aaaggagaaa tatttatgaa tacaggattt ccaaaagaat ttttatgggg
     2401 agcatcatct tctgcatttc aagtggaagg agcttgggat aaagacaata aaggaaaaac
     2461 cgtagccgac tataattctt ttaaaaagtc tcatttacag gcagatacta aagtcgcatc
     2521 agacttttac cataattatg aagaagatat tgaattaatg aaagaattag ggatgaaaac
     2581 atatcgtttt tcaatttcat gggcaagaat aataccagat ggtgaaggag agataaacca
     2641 gaaaggttta gacttttata ataagataat agacaaacta atagagtgtg atattgagcc
     2701 attcgtaact ttataccatt ttgaccttcc atttaaatta gtagagaaat ataatggttg
     2761 ggaaagtaga gaaactgtct atgcttttga aagatttgct aaaatatgct ttaagcattt
     2821 tggagatagg gtaaagtatt ggcaacctca taatgaacag aatttaattg ttagagtgga
     2881 ggagagaatt aatatatatg atgaaacaga ttcatggaag atagacaaaa tgcgtgctca
     2941 gatggattat aatctttgct tagcacatgc attagcagtc aatgcatgtc atgagatgat
     3001 aaaagaaagt aaaattggag cagcagtatc atcatctgta acatatcctc ttacttctaa
     3061 gccagaagat gtatatgcag ctagaatgaa tgataatttt aaagtgtact atatgcttga
     3121 tatgcatcat tatggagaat atccagggta ttatatgaag tatcttgaaa aaagaaatat
     3181 agttcctcac atggaagatg gggataaaga gatacttaaa aaagctaaaa tggattttat
     3241 agctgtaaat tattatagga caaattgtgc agaagcttta cctgaagatt cacaacatcc
     3301 atttggatta agagaaggaa ctgttgattt tagtatgtat ggactattta aaatgtcaat
     3361 gaatcctaac ttagaggctt ctgagtatgg agcagcaata gacccatcag gtcttagagt
     3421 tgccttaaat gaatattggc aaagatatca cttacctgtt ataataacag aaaatggtct
     3481 aggagctaaa gatatattag aagatggaaa aattcatgat gactatcgta tagactattt
     3541 aagaagtcat ataaatgcat gtaaactagc tattgaagat ggtgtagaga tgattggata
     3601 ttgtccatgg tcatttacag accttttaag ttcttctcaa ggttttaata agcgttatgg
     3661 acttgtttat ataaatagaa ctgaccatga aattttagat ttaaaaagaa tcaagaaaga
     3721 tagtttttac tggtataaag aagttataga gaacaatgga atagtaaaat aaacataaat
     3781 atatattgag ggttttagta tgtacattac aaaggtatta aataattcgt tgctacttgc
     3841 aaaggatgat aatgatgaag aaataatatt gatggggaaa ggcattggcc ataattataa
     3901 ggccgggtct gagttaaata aaaaagatat agaaaagatt tttgttcttc atgatgaaaa
     3961 tataaaaaag agtattatac aattggcaac agaaatagat gcagaatatt ttggtatagc
     4021 tcaaatgata attgcatatg gaatagaaaa atataactta aaacttatga accatatata
     4081 tttggcctta actgaccaca ttgcctttgc agtgagacgt tttaaagaag gtataatgat
     4141 tgaaaatcac tatttgttcg aaatcaagga atttaatcca aaagagtatg atataggaaa
     4201 atatgctatt actgttttta aggaggttct tggtttagac ctaccagaag aagagattgg
     4261 taatatagca ggccatttta taaatgcaca acaagataat ccttatagtg atagaaataa
     4321 gagaagtgct aaaattgtaa atgctatttt gcaaatagtt cattatcact tttctattgt
     4381 atataataaa gagtcatttt attataggag atttgtaatg catctaaagg ccttttcaca
     4441 aagatttctg gctaatgaac catcaagaga aaaaatagat tttatatatg agcaagttca
     4501 aaaaaattgt aaggaagagt atgaatgtgt gaaaaaggta ggagcttata tgtataagga
     4561 atactccaga gaattaccaa gacaagagga gttatatttg atgattcata ttcataagat
     4621 tttaggagaa ttatgcgaaa atagttagaa tatcttaata tgctagtaat attataaaaa
     4681 attaaaaatt aactacactt caaatatgaa taattgcatt acttaagggg gttgtcttaa
     4741 aattgatttt tttaattaaa tttgaagctc aactcctttt ttaaaccttt atatcattac
     4801 aactttaatg tttatacgcc tgtacttcag atgtatatct actttttaca tttctcaaca
     4861 tgcaaatcta ccattttttg cagtatgtta attgaaataa acctttgagt taaccctaaa
     4921 tttattctgg acttatagga atagttccaa agatacttta aaatattttc tggaatgatt
     4981 gcttatttca catatgtttt attgtgatgt tttgatttaa taaattcttc aactctgtat
     5041 atagtaaatg tattatcatg tttgttttat tcaagcacac ttataatacg cttgattttt
     5101 tcacaatgat gaaatatact aatgggtgtt ataagtataa gaatagcatt acacttgaag
     5161 aagcaagtga taaatgaggt ataaatcctc atatgataaa ctactatttc attgaggagc
     5221 atattctaag tgatgtcaag atactacaat atgactaaat tcctagtgat aaaaaagtct
     5281 acgaatgagt gtagaaaggg gaactaagat gaataaagtt ttaattatag atgatgataa
     5341 agatctttgt attttgttaa aaaagagtat ctcaattgaa cagattgaag caaactattg
     5401 ccattctggg agagatggac tggttgaact ttcaaaacaa gattatcagt tagtagttct
     5461 agacgtgatg atgccaggta tggatggttt tgaaactatg gaacaaatac gaaaatatag
     5521 cagtattccc atcttaatgc tgacatcaaa aagcgataat tacagcaaag tgcatggatt
     5581 gcgagctggt gcagatgact atctaaccaa accatttgaa attacagaat ttactgctcg
     5641 tgtactgtct ttaatccgac gttacacccg ttttaatcca aaaaatgagg aaccacagca
     5701 actttctttc aaaggtttgt tgattgacct tgaccagcat acagtgacta ccaatggcga
     5761 acctatgttg cttccaccca aggaatttgg tgtacttctt tactgtgcaa ggcatcaggg
     5821 taagattttg acaaaacagc agatttatga agccgtgtgg ggtgagccat atgtatatga
     5881 tgacagcaat atcatggcta ttatcagtcg cctaaggaaa aaaattgaac tagacccatc
     5941 atctcccatc tatttgcaaa caataaaagg aattgggtat caattcaaca cggaggtatg
     6001 aaaatggatg aacaaacgat aattcttttc ttactcttgc tgtcgattgg tctacttttt
     6061 ctttccatat ttgctttttc caagcttggg catatttaca agcgcctaaa agatatagag
     6121 gagatactgg ctgatgtaca aattggagac gaaaatcgta aaatactaat taagccatat
     6181 gatgttatgg ctccattggt ttatcagctt aatgagattg tctacgatta tgaaaataag
     6241 ttactttctc tgaaaaagtc ggataaagct agcaaacaac ttatgacaag tttatctcat
     6301 gatgttcgta caccattaac aactctgatt ggttatctgg atgctgtaca cagtggtata
     6361 gttatcgaac aagagcggga ggaatacctt gaaatcgcta ggcgtagagc ctacgacctc
     6421 aaggattaca ttgatgttct ttttgactgg tttcggttga actctgatga atttacgctg
     6481 tcaatagaat ctgtggaaat tgcagagttg tcacgcaata ttttgaaaga ctggattcct
     6541 atattccatg aaaagaaact gaattttgaa attgatattc cagaaaatcg actaatggta
     6601 aacctcgacc gagatggata ttcccgtgtg gtcaataatc tggttcaaaa tgttcttgct
     6661 cacagtaagg ctcggcaaat caaaattacc atgagtgagg attccagaat ggtattactc
     6721 cgtgttgagg atgatggtgt tgggattgcc agagaaaatt taccacatat ttttgagcga
     6781 ctgtataagt gtgataaagg acgttcagaa aaaggaagtg gtcttggtct atctattgtc
     6841 agtcaaatgg tggagcgaat gggtggtcag atttccgtag aaagtgagat tggaaagtat
     6901 accatattta cagtgagctt accattaata taaaattgtt tttcccccat gtgacatgta
     6961 tacacagggg gattttttgt aagttataaa gttgcaaggt taatacaagg ttgttgcaag
     7021 gttaatgtaa gattgaggtg gtatgataac tttaataaac aaaggaggct tttattattt
     7081 atgaataatt taattattga aacaaaaaat ctcactaagc aatatggtgc tcaaaagagc
     7141 gtttctaatt tgaatattca cgttcagaaa ggtcgcattt acggattgct aggaagaaac
     7201 ggtgcgggta agacgacaac tatgaaaatg ttattgggtc taacaaaacc aactactgga
     7261 gaaattaaaa tatttggtca ggacattcac aataacgaga agaagcttct tcctcgaatt
     7321 ggatgtttga ttgaatcacc agggttttat cctaatctga caggaacaga aaatctcaaa
     7381 atctttgcaa ggcttagagg aataccaaaa aaaaatgcag tagaatcagc actggatgta
     7441 gtcggtttgc caaacaatga caaaaagtta ttttctcaat actctcttgg tatgaaacag
     7501 aggttggcga ttgctcttgc cattatgcat gacccacaac tgttgatttt ggacgagcct
     7561 atcaatggac ttgatcctat tggtattgca gaagtaagaa cgtttattcg tgatctctgc
     7621 acagaacgag gtaaaactat tttgatttcc agtcacattc tttctgaaat tgccttgttg
     7681 gcagatgaca ttggcattat tgaccatggt attttgttgg aggaagaaag tcttgcccaa
     7741 ttagaagaaa aaaacagtaa atacatacat tttgctgtat ctgatgcagc acaggcagca
     7801 aggcttctgg aaacccaact taacctgaaa aatttcaaag tagcagataa ttacaatatt
     7861 atgctatatg atacagatgt ttctgttgca gaaataaatc gtgctttcat tctaaacaac
     7921 attgaggtat cagaatctca tttatgcgaa gatactcttg aagattactt caagaaagta
     7981 acagggggtg aaggaattgc ttgatataat ttcatgtgaa ttttcaaaac tcaagcggtt
     8041 aaaatttata ctgatttcta ttttaggtgc ttgcttattt ccaattcccg caacaatttt
     8101 gattgcaaag gacaacttgc catttgagca attgttcaaa ttagttgtca attttggata
     8161 ttttttgcta ttaccaattg tattaagtat tgttgctagc caactatttt ttatagaaag
     8221 agataatgat gtattaaaaa atcttgcaac agtgccagtg caaaaaggaa aactggctct
     8281 ggcaaaattg gctgtgttgc tgtttatatc attgttctac tctgttgcag ggttaggagc
     8341 tactatcatt ggaggtttca ttgttggaat agtagagggg gttgctgtaa aattaggaat
     8401 gagcattgct tttggaatta tgttttttgt tactgtactt cctgttgtgg tactcattgt
     8461 atatttcaat cgcagctata ttttttctat tatactttct tttgtatatg ctattttcaa
     8521 ttttagtata tccttaaata ttatcaattt tgaaccaaat aacccaatat taagtgtatt
     8581 acctgctcct gttataatgc gttggtggat ggcattttgg ggagatccca ctggcgaata
     8641 cacggcactt agacagccat atttgctctc aactcctgct tgtgtaggta ttttatgttt
     8701 gataaccatt atagcagtcc tgctgatttg tatgatatac aaaaagcagg agaattagga
     8761 ggtatctatg cttgatataa ttactacaga atttcaaaaa ataaaaagat ataatattct
     8821 atggattggt gttgttgctg ttctgttttc agcactattg gcagtttttc aggttgtaag
     8881 ttcacacggc agtgaccctc taacgtatga gagctttgca aacggagtta tctggaataa
     8941 tttcagtctt agttttccat ttgggattac acttattgga ggatatctta ttaaccgtga
     9001 atatacagac caaaccttaa aaaacatcct aactgttcct atttcattac gcaaattgct
     9061 agtgggaaaa ttaattgcgg ttggaggaat tactgtattg ttaagtcttt tcagcttctt
     9121 atgcacattt attttggggt tgttattttg taatatcaat atatctgtta cactcgttgt
     9181 aaaatcctta gttcagattt tgtctattaa tctgtattgt tatgttgctg ttttgccgat
     9241 tattgcttat tttggtcgta aacaaaatgc atttctgacg ggtgtaggga ttgcatttgt
     9301 atacggtttt tgtggtgtgt ttgttgctgg aagaaatctg acagactttt atccaatcac
     9361 agcaggtctt ggtctagttg gatacaacaa tggtgctggt actgtttatc agccacttat
     9421 cggtgctatg actttgattg taattctaat cttgacaact attctacttg tatttacacc
     9481 taattatgat aaagttatga ccatatctaa gaaaaaaggg ataaagcata aaaagtattc
     9541 tcataaataa cataaatatt gtttctatta tttaagttgc tagacagtga aaagaaaaaa
     9601 tctgtcgtac agtggctata tgctcatgta ttcaagaagc tacagttttg atttttcata
     9661 actgtagctt ctttttgtat ttattaaatc acgactatca taatacttgc aataggtata
     9721 gcgacaagag agagtactgt catatgtgtt ttatatatta cattaagtat attctaaaat
     9781 atatgtttta aataatatat ttgaaaatag gaatatttaa ataaaaaaaa taatattata
     9841 tatagttgac aaaaattggc aaatgatata atatataaat atagaaatag taatcattac
     9901 tattattaaa aagggaggaa ttaattatga acttaaaagg aactaaaaca gaaaagaatt
     9961 taattgcagg atttgcagga gagtcagaag caagaaataa atatacatac tacgcttcaa
    10021 aagcaagaaa agaaggttat aatcaaatag ctgctatatt tgaagaaact gctaacaatg
    10081 aaaaggaaca tgctaaatta tggtttaaac ttttacatga tggtatgcct tcaacagagg
    10141 aaaatttaaa ggatgcagca gcaggtgaaa actatgaatg gactgatatg tatgcaaaat
    10201 ttgctaaaga agctagagaa gaaggatttg acaagatagc ttatttattt gaagctgttg
    10261 gaaaaataga gaaagagcat gaagaaagat acttaaagct tttagaaaac ttaaatgaag
    10321 gaaaaatatt taaaagagat gaagaagttg tttggcaatg tcaaaactgt ggtcatgtgt
    10381 atgtaggtac tgaagcacca gaaaaatgcc cagtatgtga ccatccaaaa gcatatttta
    10441 atataaaagc tgaaaattat taaaaattat cattattaaa aaggaatgtt aactatgaaa
    10501 ttttctaaac aacgagaact gattttaaat gaaatattaa ataatccggt tcatcctact
    10561 gcggattacc tatatgaaaa cttaaaaaaa gataatccaa atttaagctt aggaactgtg
    10621 tatagaaatt tagctcagct aacggaacat ggctttataa gaaaggttag tattccaggg
    10681 tatccagata gatttgatgg tcgaatagat aatcactatc atatcatatg tgaagtatgt
    10741 ggagaggtat acgatttaga atccgaagtt cttaataatt tacaagaatt aatatctgag
    10801 gagacagaca taaaaataac atcttataac ataagtttta aagggatttg taataattgt
    10861 aaaaggtgta gccaagtagg ttaattttca aattctaaaa ataattaatg aggtgagttt
    10921 atgtgtagtg aacaaaaatt ttttatatgt gagacttgtg gaaaccttgt aggaatgata
    10981 caaagtggtg gtgtacctat tttctgttgt ggaaaaccta tgaaggaact tgttcctaat
    11041 acaacagatg cagcagttga aaaacatgta cctgtaattg aagtggatgg aaataatgtt
    11101 actgttaaag taagtagcac aactcatcca ataactaaag aacatcatat agcatgggtg
    11161 tatcttatga ctgaacaagg tggtcaacgc aaatgtctag cagtagatgg agaacctgta
    11221 gttaaatttg ctttgaatga tgatgataaa gtgatttctg cctatgctta ctgtaattta
    11281 catggattat ggaaggctga actgtaatat ttaaataaat tgagtcaaaa aggaggctat
    11341 aaatgtattt tcatacaaat agcctcctat aaatttatat aaattcaagt tttaattttg
    11401 tttaatgtca atataatata aggagaaaat aatatgtcaa tttataaatg tagtgtttgt
    11461 ggatatattt atgatgaatc taagaatgat aaaacatggg atgaattaag tgaagattgg
    11521 gaatgccctg tatgtacaaa aggtcgtagt tattttggaa agataagtac tgtttactat
    11581 gaagaagatg aaaagatagc agaagacata gttgaagatg aatctaaatt aaatactgaa
    11641 aaagagggag atttaaatta cttaagtaca tatttaagaa gagatgatga agttgagaaa
    11701 catatggata taattcacga aatggctgtt acaggaaaat ctataataga acctatgaga
    11761 actaaactac cagtaatttc ttgggatgat attttaatta tgggtgctca attaaatcca
    11821 ttacctttaa atgagcatga tgaagtaaat acaacaacaa taataggaaa aaaagcaaag
    11881 aaacctatga ttatagaaaa tccagtttat atttcccaca tgtcatttgg agctttgtct
    11941 aaagaactaa aaatagcttt agcaaagggg gcagcccaaa ataagacagc tatgtgtagt
    12001 ggtgaaggag gtatattacc agaagagaag gaagcttcat acaagtatat ttttgagtat
    12061 gtaccaaata aatatagtgt aactgaagaa aatcttaaaa attctgatgc tatagagata
    12121 aaaattggtc aaggaacaaa acctggtatg ggaggtcatc ttcctggcga aaaagtaact
    12181 gaagaaatag caaaagtaag aaataagcct gtaggtcaag atgtaataag tccatcttgt
    12241 tttgaggaaa tacaatccaa agaagattta aagaaacttg tagatgaact tagagaagtt
    12301 tcagaagggc gtccaatagg tgttaaaatt tcagcaggtc atattgaaaa agatatggaa
    12361 tttatagctt atgctaaacc tgattttgta actattgatg gtcgtggtgg agctactgga
    12421 gcaagtccaa agttgcttaa agatgcaact tcaattccaa caatatttgc tctttatcgt
    12481 gctagaaaat acatagatac acatggatta gatatagatt tggttataac aggtgggctt
    12541 cgtatatcta ctgattttgc taaggcaatt gctatggggg ctgatgcagt tgctattgca
    12601 agttctgctc ttatggcagc ggcgtgtcaa caatatcgta tatgtggttc tggtaagtgt
    12661 cctgtaggag tagctactca ggatgaggaa ttgagaaaaa gattacatat agaaaatagt
    12721 gctaatagag tggcaaactt tttaaatgta agtttagaag aacttaagac ttttgctaga
    12781 atttctggtc ataaagatat acatgattta agtgttgatg atttatatac tgttaactct
    12841 gaagtatcaa attatacaaa catacagcat gtatagattg aagtttgaaa ctattagatt
    12901 ttagatagct ataaaagtaa atgataaata tattttataa aagagagtgt cttaaaatga
    12961 actttttagt tcataagtca ctcttttttg tatgaagcca aatatattta tcattttagc
    13021 aatgaaaaag aatcttatct ctttattttg agatagactc cttctattta tgaaaattaa
    13081 agatattaaa ttgtaaaata ttctgtgatg tcgtactatt aaactaaaga ttctatactt
    13141 taaagtaaat aaaaatttaa ggagtgatta ttatggaaag attgaagata caagttttag
    13201 tcgacaataa tacttatatt gatagatact ttgttggaga accagcagta agctattata
    13261 ttgagattga tggaaatagg attttgtttg actcagggta ttcagatgta ttcatatcaa
    13321 acgctgaaaa attaaatata gatttaggaa atttaactca tgttgttttt tctcatgggc
    13381 ataatgacca tacaagaggt atccagtttt tagaaaatag gtatgattta tctacagttg
    13441 aacttatttc acatcctaac tgttttatac ctagaaaaca tggagagaaa agcataggag
    13501 ctccattttc tgctgaggaa attaaaaata tttttatgta taatcctaag gataagccat
    13561 ttaatctaag caagaattgt gtgtttttag gtgaaatacc ttctataaat gattttgaaa
    13621 aaagagctaa aattggtaaa tgtaaaatag gagatttgtg ggaagatgat tatgttttag
    13681 atgattctgc tatagtttgt aaaactgata aaggtatttt tattgttaca ggatgttctc
    13741 atagtggaat ttgtaatata gtagagtatg ctaaaaaggt atgtggagat gatagagtta
    13801 ttggtatact aggtggtttt catctttttg aattgaataa taggttagat agtacaatac
    13861 aatatttgga taaagaagaa ataagaatgt tgtacccttg tcactgtgtt tcttttaagg
    13921 taaaagcgaa gatgaatgaa atattgaata taaatgaagt tggagtaggg ctgaccataa
    13981 gtatatagta tttgaaagta tataagattt aagttgaagg ttaaaaagat attttctata
    14041 gaaaatatct tttttgttac gtttatttta agagaaatca atatactttt atatgaaata
    14101 ttgtaaaaaa atttattttt aatgagaata tattttcatt aataaacaat tttattatgt
    14161 attaataatg ttagaataaa tataaataaa tcaaagttac ttacattaca gtatattttt
    14221 tcacaaaata tattgattta ttcttttaaa gtttgataat tccaatgaat gtaataaaag
    14281 aaggaatttt atactatact attattatta taaaataata aagaaaaaat tatataataa
    14341 taaagaaaaa atgtataata tttaacatta aaaaagatat ttcataaaat taaaaaacaa
    14401 agtgaatttg aatactattt aaaaaataaa ataaatatat aaaaaatatt taatataaag
    14461 gtagaaataa gttgaaaatg taaagtcatt ggaaaaatat agttgaattt aatgagcaaa
    14521 aatgttattg ttttatcaat aaagtgttaa aataaaaact tttaatatca ttttagggag
    14581 gatgatttca tgcaaaaaag tgtaaagaaa gcaaaagtaa cgggctctat gttagccatt
    14641 tttggtgttg caagcgttct ttttagttca catgcaggag gaggatttgc tacagggaat
    14701 caagaaactc agtactatgt gcagtatgga tggacagcac cacttatggc aattttagca
    14761 atgattatac ttactgctac catgagagaa gttatcataa tgtataataa taacaattgt
    14821 aggaattata aggatttatt ttgtgagctt tggagaccat atccaaaact tgaaataata
    14881 tgggagatat attactatct aatggtttta atagctgtaa gtgctgttat agcaggtgca
    14941 gcagcagtat ttcaaagtat aggagtaaat tattttgtag ctgtatttat tattggagtt
    15001 gtacttcttg tctttactat atttggtgct atgcttgtat ctaaagcagc tacagcaatg
    15061 acaatagcta ttctagtatg tactcttact atttttattg ttggtataaa agctaaagta
    15121 ccagaaataa ctgaaatatt atcaaataga acgagtttca cacctggcta tatcaagcca
    15181 atacttaata cttttattta tgcaggtttt caatcggttg ttatacctac acttgcagga
    15241 tgttcaagac cacttcttaa gaactcaaaa gaagctacta aggcaatgat aatttcattt
    15301 gttatgaatg caattgcatt aggacttgct gtaactatgc taatgggatg gtatagagaa
    15361 attattgctg ctgggcaaac aacactccct actctttatg ttgcaggtca atctggaaat
    15421 catactatat acataattta taatgttgca ttattccttt gtttaatgtc tacaggagta
    15481 actactatat ttggtcttgt aaatagattt gaagaccata aagcactttc tttcctttct
    15541 tcaagaatga aaagaagggt atttacagct tgtgcaataa tggtagtgtc aatgcttata
    15601 tcacttacag gattaagtaa tattgttaaa tatggatatg gatattgtgg ttatcttggt
    15661 ctattcacta tagtaattcc attccttaca ctaggacatt ataagaataa gaagtttgca
    15721 aaggaaaatc cagaagctaa atggccagcg gaattaaatg aaaatgtaaa ttagatgtag
    15781 tagcaaaaaa tattgctaaa gagaatacta tagataagaa tactatagat aatatatctg
    15841 catgtttatt aagattattt gcaaaatcta atattaaaca actataaaca agcaatataa
    15901 aactaagaat aaaagataaa aagagggtgt ctcaaaatgg acttttagtt tataaggcac
    15961 tcttttttat atgaagtcaa atatattttc attattttga caataaaaga gactaatcct
    16021 actttggata gcctcttttt tctttaatat aaaatagtat aacagcaaag tatagattga
    16081 tacatgtttt ctgtagcttt ctatatatta cagtgtttgt gtggcattgg tattttaagc
    16141 aatctataaa ttataattct ataattcttc tactaatttt agattctata gccgtctcta
    16201 tttcacttaa cattcgttca tttttagggt caataaaagc acatgtatag ccttcttcac
    16261 cagctctacc agttctacca atacggtgga tgtagtcttc tggtgtttct ggtaaatcgt
    16321 aattatatat atgacttact ccagatatat caagaccacg agaagctaca tctgtagcta
    16381 ttaagtattg tatatctaaa tttctaaatg atttcattat tctttctcgt tttgattgag
    16441 caatatcact atgaagtttt tgacaattat atcctcttat agcaagagct tcttcaagat
    16501 tgtcaaccct tctcttagtt ctacaaaata ttattgccat aaaaggatta tcttcatcta
    16561 gtaccttaca taaggcatct aatttttttc tatctgtagt ttctactact tcttgtttta
    16621 tattattaag agtgatttct tctttttgaa tagaaactac aacaggattt tttgtgtatc
    16681 tataagctaa tttttttact tgagaattta tagttgcaga aaagcatagt gtttgctttt
    16741 tgtttgaagt ctcttttaaa atagcttcca cttcattttt aaatcccata agcaacattt
    16801 ggtctgcttc atctaaaaca aaagtcttta acttactaag gtctattgtt tttctattta
    16861 gatggtctaa aagcctacct ggtgtagcta taatcatatg aatatttcct tttagtttct
    16921 ttatttgaga accaatatct tttcctccat atgctgcaag aatacttaca tctttagatt
    16981 ctttaagttt catagcttct tctgttattt gaatagctag ttctcttgta ggagaaagta
    17041 taagtacttg tatatcattt atatctaatg atatattttc aaatatcgga agtaaaaaag
    17101 ctagtgtttt tcctgttcca gtttgagctt ctgctattac atcttttcct tcttttatta
    17161 gtttaatact ttctttttgt ataggtgtag gtgttgttat tcctgatttt tttagaatat
    17221 ttattgtatt ttcactaatc cctaagcttt tgaaatccat tgttatctta cctttcatga
    17281 a
//
LOCUS       DAEMGG010000085        17260 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEMGG010000085 DAEMGG010000000
VERSION     DAEMGG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.085"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(151..675)
                     /locus_tag="KPK24_002549"
     CDS             complement(151..675)
                     /locus_tag="KPK24_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2300216.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(740..1492)
                     /locus_tag="KPK24_002550"
     CDS             complement(740..1492)
                     /locus_tag="KPK24_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG2300217.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1523..2821)
                     /locus_tag="KPK24_002551"
     CDS             complement(1523..2821)
                     /locus_tag="KPK24_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG2300218.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVKSTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2838..5102)
                     /locus_tag="KPK24_002552"
     CDS             complement(2838..5102)
                     /locus_tag="KPK24_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG2300219.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5357..6175)
                     /locus_tag="KPK24_002553"
     CDS             complement(5357..6175)
                     /locus_tag="KPK24_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2300220.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6168..6866)
                     /gene="cbiQ"
                     /locus_tag="KPK24_002554"
     CDS             complement(6168..6866)
                     /gene="cbiQ"
                     /locus_tag="KPK24_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG2300221.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6869..7147)
                     /locus_tag="KPK24_002555"
     CDS             complement(6869..7147)
                     /locus_tag="KPK24_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2300222.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7137..7889)
                     /locus_tag="KPK24_002556"
     CDS             complement(7137..7889)
                     /locus_tag="KPK24_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG2300223.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8071..8263)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8395..10167)
                     /locus_tag="KPK24_002557"
     CDS             complement(8395..10167)
                     /locus_tag="KPK24_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG2300224.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10181..11110)
                     /locus_tag="KPK24_002558"
     CDS             complement(10181..11110)
                     /locus_tag="KPK24_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2300225.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11339..11527
                     /locus_tag="KPK24_002559"
     CDS             11339..11527
                     /locus_tag="KPK24_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300226.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11520..12221
                     /locus_tag="KPK24_002560"
     CDS             11520..12221
                     /locus_tag="KPK24_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300227.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12223..13146
                     /locus_tag="KPK24_002561"
     CDS             12223..13146
                     /locus_tag="KPK24_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2300228.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRVQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13303..14226
                     /locus_tag="KPK24_002562"
     CDS             13303..14226
                     /locus_tag="KPK24_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300229.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14219..14956
                     /locus_tag="KPK24_002563"
     CDS             14219..14956
                     /locus_tag="KPK24_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300230.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14967..15740
                     /locus_tag="KPK24_002564"
     CDS             14967..15740
                     /locus_tag="KPK24_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300231.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(16281..16889)
                     /locus_tag="KPK24_002565"
     CDS             complement(16281..16889)
                     /locus_tag="KPK24_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG2300232.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17067..17183)
                     /gene="rrf"
                     /locus_tag="KPK24_002566"
     rRNA            complement(17067..17183)
                     /gene="rrf"
                     /locus_tag="KPK24_002566"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5560 a         2998 c         2181 g         6521 t
ORIGIN      
        1 catacaatat atagatttcc acaatacaaa taaattaaaa agtgaaatgt atttgtctaa
       61 aaacataata acatttcact ttatcctcat ttttaagtaa tatatatttt taatttttat
      121 ttataactaa atctagtttt tatataaatt ttagtttttt tctacatctg agtaatctat
      181 tccaaaagtt tctacagtaa cagatttcat aatttgtgga gtttgtggct tatcagctga
      241 atctgttcta actgatgcta tttcatcaac tgtatccata ccttctatca ctctaccaaa
      301 acctgcatac tgaccatcta agtgtggtga gtttttatgc attatgaaaa attgagaacc
      361 agctgaatta ggtgccatag ttctagccat tgatatgact cctctttcat gttttaaatt
      421 gttagtaaat ccattaccac taaattcacc ttttattgag tgacctggac cacccattcc
      481 tgtaccattt gggcatcctc cttgtatcat aaatcctggt attactctat gaaatattat
      541 tccattgtaa tatccttcat ttactaatga tacaaagttt ttaactgtat ttggagctat
      601 atttggatat aactcaattt tgatttcttt tccattttcc atttctatag ttactatagg
      661 atttttattt tccataattt attaccttcc ttttcattag ttaaattaca taattacatt
      721 tattatatta tactctaaat taaagtctat caagtatatt ttttcctatt tcatatcctt
      781 cttgataaat agttttacaa aactcatcat caaagttaag cgtgtcttta ggagcaaatt
      841 gagtattagg tctaactact attattttat cttcatcata tactttttta atatcttctg
      901 gcaactcata attatatttt gtggatatta aaataatttt atccacatta tccccaatta
      961 atggttcaat taatgcactc ctaattaaac ctccatctaa tttgaaacca tcataaattc
     1021 cttcttgcac atctttcaca aattgttctt taaatccaag agttgcgttt ggatttgatg
     1081 gtaacaaccc actgtatgaa atgctgataa tggcttcatt acgaggcatt tttgatacat
     1141 caacaatctt ttctgatact actttattat caacttcaac ataattaaca taaaagttca
     1201 tttcttctat attactatca tctagttccc ttaggttgtc tatagcagga gaattatcca
     1261 cagtattatt tactattttt acatttccat tctggatatc ttttatattt gtccacattt
     1321 tctccaagtt atcaacattc cctgtgtaca aataatatcc atttattgct cctattgatg
     1381 tccctgaaat cgaataaaat ttatttattc ctccatcata caaggcttta actactcctg
     1441 cttgataagc cccttttgct cctccacctt ctaaacataa tcctaatttc atagtctcat
     1501 ctccaaattg tttatcttat atttatattt caaaaaactt actaaatgtt ttatatatac
     1561 tatagtggtc ctcaacattt cctaattctc ttatagaatg cattgctaaa attggacttc
     1621 ccacatccac agatggtata tctatatgag tagaagatat tggacctata gtacttcctc
     1681 ctctttcatc tgacctatta acaaattctt gatactcaac attagcttct ctacatatat
     1741 ttttataaat agctatagaa tatccatcac tagtatacgc ctgactagca tttatcttta
     1801 taaccggacc ttttcccatt attggtttat tagttgggtc atgcttttct ggaatgtttg
     1861 gatgaatagc atgagctaaa tctgatgata tcataaaaga tgaataaata gactcaaaga
     1921 atccctctct atccttaccc attgatatac aaattctctc caatatattt aacaacatgt
     1981 ttgaatctgc accttgttta gtagagctac caacttcttc attgtcaaat acagccatta
     2041 catttacacc atgttgacct ttagtattaa caagagcatt caaactagca tgtaccattg
     2101 caagattatc ttgtctccca atagatataa attcatcatt agcccctatt aaacttcctt
     2161 tttcaaattc atataaaaat aaatcaaaat ctataatatc gtccatattt acatctaagt
     2221 tagatgctat ttgctttata agaaaattat ctttttctaa tgtatcattt aacatcccaa
     2281 ctaaaggtaa catatctttt tgcttattat atgaatgacc atcatttata cttctattta
     2341 aatgtattgc tagatttggt attatacaaa ttggcttttt aaagtctata agcctttcat
     2401 ttggttttaa tatactatca ccttttaaaa ctactctacc agctatccca agaggtctat
     2461 ccaaccatgt acttaatatt gctcctccat agcattctgt atttaacttc aaataagatt
     2521 tttcagcttc tatttctgca tttggtttaa tactaaatgt aggagaatct gaatgagagc
     2581 ctattattct aaatccatct ctttctatat cttctgaatt tactacaaaa gctactatag
     2641 ctgataaatt cttagtaaca taatactttc caccaactct tagattccat ctttgattca
     2701 aaactaattc ttcaaatcca tttttatcta agatttcctt agtactcttg actgcatgaa
     2761 aagacgacgg actatcatat atgaagtcta ttaagttttt agcaaagttt atactatcca
     2821 ttcctcattt acctccatta taaaatattt agtgttgtat attctttatt tattttttta
     2881 attccctgtt ggtcaaaagc tatagtaaca tctgtaccaa ttatagaaac cactgtccca
     2941 accccaaatt taggatgatg taccttagaa ccaagtttta tatcatctgg attagtttct
     3001 ttattagaat cttttattgt agcattaact ttatctgcaa ctttagattt gcttatagta
     3061 ctcatatact ttttagtata tttatctaac atattataac ttgctttaga atagcttagt
     3121 tcttttttta ctttgtacaa tctttctata cattcttctg gcagttcttc cataaatctt
     3181 gaagctattg caacatttgt tttaccatat aatgttctct tctctgtcaa tgacatatat
     3241 agctcttctt tagctctagt tataccaaca taacaaagtc ttctctcttc ttctatttgt
     3301 gaatcactca tagattttat agctcttgat ataggaaata acccttcttc catacctgtt
     3361 aaaaacacaa cagggaactc taaaccttta gatgtatgaa ttgtcataag cgaaacccta
     3421 tcattgtctt cttcatcatt tgattcagat gtaagtgcta tacttgttag gaatgtctct
     3481 aagtctttat tttcataagt atcatcatta ctactttcaa actccaaagc tatagaaata
     3541 aattctctca aattatcaat tctgtcttga gcttcttcac ctttgcctgt taaatctttt
     3601 tcatttttat ttcttatctc tacaagctcg tccatatatc ctgtagtgtc caagactttt
     3661 tctataagtt tgctaacagg ataaacttct tttatagttc taagagtccc tataatatca
     3721 acaaatccac ttatacttgc ccttgcctta gtagatatat ctgaatttgt ttctatatca
     3781 attaatactg agtatatact ttcctgtttt aagttagctc tatcttcaat tttttctatg
     3841 gttctaagcc ctatacctct tctaggaaca tttataatcc tttttataga tatatcatct
     3901 tgtggatttt gaataactct taaatacgcc aacaaatcct ttatttcttt tctctcatag
     3961 aattttgtac caccatatat attgtatgga atttgacttc tatttaaagc gtcctctaca
     4021 ggtcttgcct gtgcatttgc tctataaagt actgcaaaat ccttataagg tctattttct
     4081 tttctagcta tttgtgcaat tgaatcagca atgaaatcag cctcttctat ttcactacca
     4141 gttaattgta tctttataag ttctccttct tttttttcac tccaaagctt ttttcttttt
     4201 ctttctatat tattagatat aactttatta gctgcatcta atatcacttg tgtagaccta
     4261 taattttgtt ccaactttac aacatgtaca ttgtcataat ctttctcaaa ttcaagtatg
     4321 tttcttatat ctgcacctct ccatccataa atactttggt catcatctcc aacaacacaa
     4381 atattttgat gttctcttgc taataatttt atcaattcat attgagcttt acttgtatct
     4441 tggtactcat ctaccattat gtatctaaat ctacttctgt aatatgctaa cacttcatca
     4501 ttagccttga aaagttctac agttttaagt attaaatcat caaagtcaag tgcactattt
     4561 ctttttaaac ggtcttgata tagcgcataa atatctgcaa tcttaaccat tctattatct
     4621 gccatattga tatctttaaa ctgctttgga gtataaagtt tatcttttgc tcctgaaatt
     4681 gcacttataa ctgcttttgg ttcaaacact ttatcactta aatttaattc ttttagacag
     4741 tctttaacca atgtaacttg gtctgctgaa tcataaatta caaaacttct attgtaacca
     4801 atcttattta tatcttttct tagtatacga acacagcaag aatggaatgt acttatccac
     4861 atatccttag tttcaggacc tatattttgt tcaactctct ctctcatttc atttgcagct
     4921 ttgtttgtaa atgttatagc taatatattt ggtgcttgta cacctttatc ttctataaga
     4981 tatgctattc ttgttgttaa taccttagtt ttacctgaac cagcaccagc cagtataagt
     5041 actggacctt cagtcttttc tactgcttct ctttgagcag gatttaatgt atctaaattc
     5101 atatttctcc tcctgttaac agccataaat atattttaat ctaaaatatg aattacttca
     5161 aattataatt atgtattata ttaatagatt atttccaata tattacaatt aaataacatt
     5221 tttccatata atttatttca ttgtttaata aaaaaaataa ccttatgatt tggggaatca
     5281 taaggcacct ttaaggggtc taggttaagg gttattatat ataaatctat ttttttaaaa
     5341 taggtttagg gcttaattat ttatttgtat taagttctct ttccttccaa tacttataca
     5401 aatcttcttc tttcttaaat aagggtaaat tcatatttct atgtactttt actaaccatg
     5461 gggattctag gccagcttgt ataatattat tttcatttat aaacacattt tctgcttttc
     5521 cttcatcaat caaaatcccc ttatccaata catatatgta atcacatatt tcatacatta
     5581 aattcatatc atgactggat attactattt ttatattatt tttatttaat ccctttatta
     5641 tatctacaat agacctagta cttactgggt ctaaaccagc tgtaggttca tcaagtaata
     5701 caatttcatt ttccattgct ataactgatg ctattgccac tctttttttc tgtccatagc
     5761 taagaaaatg tactggcctg tctataaaat ctattccatt aacagctact agagctttgt
     5821 ttattctctc tttaattgtt ttttcatcca taccaatgtt tctcattgca aaggctatat
     5881 catcatatac ttttgaataa aaaatttgtt tttctgggtc ttggaacaca atacctacat
     5941 tttttctaag gttgtataaa cttctcttat catattttaa cttttcttct ttaaaaagaa
     6001 tttctccaga tgtaggcttt aatataccca ttaaattcat aaataatgtt gattttcctg
     6061 aaccatttga acctattata cctataacat ttccctttga aaaatccata ttaatgttta
     6121 atagtgcatt ggtatttttt tcatattgat acgttaaatt atttatttta aacatcatta
     6181 tcccctacta tgtgaaattt accatcatac aattttatgt ctaatgatat acacatatca
     6241 tcataccttg tcataagtct tttgtacagc atacttccaa gaatacctaa ggatttatat
     6301 gaagttttta aatttatata tccaaatctt agctcttgtg acttgtatat ttctgaaaat
     6361 tcttctaaaa atatgaatat aaacctatat attaacattg ataactcgac aaaagtgtca
     6421 ggtaaatgaa gttttttaaa aaagaatatt aattgattaa aaggtgttgt aagtatacaa
     6481 aaatacacac aagtcaaaca agacatcgct ctaaataaca catgaataga catgtcaata
     6541 gacgcctttg atattcctac attaaaacta aaaaattcaa aactatataa taaatctgct
     6601 ttattaaaag atatatttac aagagttatt cctatactaa gaaataaaaa gtacattggt
     6661 attctaagta attttaaata attttttaaa tctattccag ccacacaaac aaccaaaata
     6721 ctcattaaaa tcataattcc acttaaaata aatatatttt gtattaccat agaagcaatc
     6781 agaaaaatta ctcctatggc tacttttttg tttggattta ctttactcaa tctatttgta
     6841 tatgcatatt tatcaattat aagcatgatt aacttctctt tcctttttga agacctaata
     6901 tgtatcctat tacaccagcc cctaaagctg cttgtgcaga gaataataaa ctttctattt
     6961 ctccacttgg tggctcatat ggactgctaa accatggttt gtaatttgga tttatcttag
     7021 taatttcact ttcagcttga ccatctgctc ctccatattc agcacctgag ttgacaagta
     7081 atgggaatat aataagagct actactaata aaagtaataa tatgttagtt tttgttttag
     7141 cactcattag ctttaactcc tttatcatta ttttctagta tgcttttttc tccgttttct
     7201 gtaattaaat tatatacaat tacagtaagt ataccttctg ctattgctat tggtatttga
     7261 gtcataaaga aaattcctaa aaatttaatt gctgaagcca ttaccccacc tgatgggtct
     7321 gggaaagcca gtgctaattg tattgaagtt attgtatatg tggctaagtc tcctattgct
     7381 gctgcaagaa atactggaat agcattattt ccatcttttt tctttaaagc cttaaatatt
     7441 aggaatgata cgattggccc tattatcgcc attgaaaaag cattagctcc taaagtagtt
     7501 attcctccat gtgctaataa caatgcttgg aatattaaaa ctatagttcc aagaacaaac
     7561 attacgcttg gtccaaacat tattgctcca agacctacac cagtaggatg tgaacaactt
     7621 cctgttactg aaggtatttt tagtgctgat aatacaaaca caaatgctcc acataaagct
     7681 aataatactt tctttttagg gtcttgcttt actattttac ctattgattt taatccgaat
     7741 aagaaaaatg gtataaatat aactccccat gctatagacc attttactgg taagtaacct
     7801 tccattatat gcattgcatt tgatgtaact ggagttaaaa tgattaacat aagtgcagct
     7861 ataaccccta atttaatatt ctgtttcatc ttagtatccc tctctccctt tctaataatt
     7921 aaaacataat aatacatgtc tgatttattt aataaaataa aaaagcctaa ctcgatggtt
     7981 aggcttctaa ttttccacaa tctaaataca aacacataaa acaaacatat ctgtataatt
     8041 attataagaa actttctcca tccccgaaga aattacttta atattttagg caggtctcct
     8101 gacttagctt catcctactc ctcttccttc ccatgagata taaaaaatat catcatagtg
     8161 gctaacaaaa gtttcgtctg catcacagta gcgggggctg taaaggaatt taaccttttt
     8221 ccctattaat cttgcttaaa gaacctataa tattatatat ttaattttta aattcttctt
     8281 aaattatttt ctatataaat cattgtatac cttttctgtg aaaatttcta cattttttat
     8341 aaattttatt catttttata tttttatgta tataaattct tttttgtaag tttattaatt
     8401 tctaattatc ttctttaaat acattgaaac tacatcagaa aatctagtta tatctcttga
     8461 atatataaaa tcatttccat atattaatct ttcagcttca agttctgatt cttttcctgt
     8521 aaatactcct aatactaata tattttgctt tctagctttt ctcacttcat tggctgtatc
     8581 tctcaaacca actatacccc tataagcctt ttctcctctt aatgttctct cactactttt
     8641 tcctatcttt acatcatttg gtcttccatc actgagaact atcaggattt tattttcttc
     8701 atctctattt attagaccac tacatataga ttttattgcc aaaccatctc tattattacc
     8761 agcacaaaaa tattcaaata tattttcact attttttata ctatcatcat aatctttaaa
     8821 tctttttaat attgtatagt ctaaaaaact agaaaatccc atcactctat ttggtatgcc
     8881 aacttctgtt agagcatttg caattatgta agcttgaatt gccacatttc cctgatttct
     8941 actttgagag ccacttgcat ccaataatat atcaataacg tatttccctt tttcattttc
     9001 tatatcttta taaaatattt tattattatt acttctacca actctccaag ctctattggc
     9061 taatatagta ccacaatcag aatatactct acttttagca ttttcttcta ttaaaattct
     9121 tgatagactt tcttttaatc tctttatttg ctgtctatac attctagcat gttctctaaa
     9181 ttttcctaaa ttattttctt tttgtcttgt tacatatttt agttgaaata cattattaca
     9241 ctcacttcta agaactccat ctgtaaagtg tattctacaa ccttcatgaa catttctaca
     9301 attttgtgtc tcaatttttt taatttcacc tttactcaaa taagtttttc cataataatg
     9361 ttctattttt gaatatatct tatttgcagt gtcttcatca acatatataa ctcggtctgc
     9421 tgaattgcta catttagaat catcaaaatc ccctacacct tctacaagca tggatgaaga
     9481 tattgaatct atatcacttt caataatatt ttcttcttca tcactataaa gttcttcata
     9541 catgaaatca gcaaatgtat caaaatttac ttctacactt tctacattat tttcatcaat
     9601 aagactattt tcttctccat ttaatatgta ttcaaaataa gttaaatata taacatctaa
     9661 tgcttttaaa atctccataa tatcttgatt ggtatctata tttcttatat cattaactat
     9721 tcttctagtc atactatcta atgctgggtg cttgtccata ctataaagta caaatgtgta
     9781 tttaatttta tccataatat tatcattgtg tattttggta aaacgtgaaa caatatcttt
     9841 ataagcattt cttcttatgt cttctatccc tggcctatct ttaatgacct tttcttcaac
     9901 tattgtattt aatataactt gaattagacc cagtataata tctttgttgt atccttttct
     9961 tattctattt gtaagatatt ttttaactat atcccaatct acatactttc ttctagcacc
    10021 tgcaattatt gcaaaatata gacctatttc ttttgaagag taatcttttt ctgatatatc
    10081 tatactttca ttgtaatcac cagctattgt ccatgataag ttattagctc tattttcaaa
    10141 ttcataatca tcatatatcc atttcataat ctcacctata ttaaatatga cttattggaa
    10201 acacatctat actctcccat ttattaggta ttctagtttt aattacatct ccaaccattt
    10261 ctttctcata tacatcaaaa gtctttcctg taagtcccat attaattgca agagttggtt
    10321 ttaatcctct tcttatagtc ttaagagatg ccataagacc acgtaaatca atagctttac
    10381 tagatatctc tccattttga gacttcaatt gtaaatctaa aaatattcct gcaaagtgta
    10441 ttagtttttc ttcatcagca tctgaaaatt cattttttaa aatcatcatt aacttatctt
    10501 cttctatagg aggaatatct attaccataa atctagatac cagtgcttca ttaagttctt
    10561 ttgttcctgc atattcataa ttcatagtcc caataaatct tgttgctgga tgtagattga
    10621 ctctctcata cccaggtaca tcaattattc ttctatagtc taaagctgaa tgtaccacaa
    10681 ctatagcatc attttttgcc atattaattt catcaaacac cccaaaacct ccattaatag
    10741 cacactcata aactgagcct cttctaagtt taacttcatt gtctataaat gtatctgtac
    10801 ctataagtgt tgaactgtct gtatttatat gaaatgatgt attccactga ggtctattaa
    10861 atatttcccc tagattatca gcaagtaagt tttttcccgt tgcctttggt cccgataata
    10921 atatattttc ttcttctaaa atagctgcta tacacataga caagatatct ttaccataaa
    10981 aataagtttt agactttgtt actctgtatt ctaaatcttt ttctaatcca taataatttc
    11041 taaaatacag tacatcttca ataagttttt catctacccc ttgcaccctt agattatcta
    11101 ttatatccat atttttaccc cttttatata aatagtattt gtttcatttc ttatcaccat
    11161 tttagcataa aaggatataa gctaaaattg attttttaat ctcccccatt ttaagtaaaa
    11221 ccatgcacca ttttatcact acaaaattaa gagtactact tactgatatt tcttgatttt
    11281 attcgtctat acgtatataa tatatactga taaattatga ggaaaggatg attgaaatat
    11341 ggaatatatg tcttgcccag aagtagcaaa aaaatggggt atttctgaaa gaagagtaca
    11401 aaaactatgt gaagaaagtc gcataccagg agtttcaaaa ctcgggtata tgtggctgat
    11461 accaaagaac gcagaaaagc ctgttgatgg tagaacaaaa agaagaaagg aagtacaata
    11521 tgaatagcgt tttgattata gatgatgaca aagaactttg tgctcttatg aaaaaatgtg
    11581 ttgagcaaga aaatttatct gctattactg cacatggtgg tgcagaaggt ctacatttag
    11641 tcgataaaaa tagacacaca ctctctcttg taattttgga cataatgatg ccagatatag
    11701 acggttttca agttctgaaa aaaattagag aaacaagtaa tattccagta attatgctta
    11761 ctgctaaaaa tgacgaggat gataaagttt ccggactacg tttaggtgct gacgattacc
    11821 taacaaaacc gttcagcata aacgaactta tggctcgtgt caattctctt attcgtcggt
    11881 atactatgct aaaccctgct tttataacag acacagattt cataataata aatggcatga
    11941 ttattgacaa gacaaaccga attgtttctg tcaataatat tcctgttcaa cttacaagca
    12001 aagaatttga tttacttgct tttctggctt ctaataaggg acgggtgttt accaaaaaac
    12061 agatttatac acaagtatgg gaagaagaat atgcttttga cgatagtaac atcatgtctt
    12121 tcatcagtaa attgagaaaa aagattgaac cagaccccaa ccatccattt tatattttga
    12181 cagttcgtgg tgtcggttat cgttttaata aggaggctta acatgaatac aagtgtctat
    12241 ctgcttttga tattggctct tagcctgatt attatagcgt accttgtttc tgtcctgcta
    12301 cgtgtccgag tacaacttac acttataaaa gatgctttag aggatataaa aaatggaaac
    12361 cttaatcgac gtattttaac agatgaaaac gacattacaa aacaaatttg ctatgatatt
    12421 aacgaaatag caatcaatag ccaatttcaa cttattcagc aaaaacaatc agaacaagcc
    12481 tacaaacagc taatgacaag tctctcccat gatgtaaaaa ctccacttgc ttctttggtc
    12541 ggttatttgg aagcagtaga aagtaaaatt gttgtaggag aggaaaaaga cgagtatatc
    12601 catgttgcgt ctaataaggc tcattactta aagaattttg tagaaaatct atttgaatgg
    12661 gtaaaattgg attctaagga acaagttttt cattttgata tttttgactt gaatgaatta
    12721 tcccgtaata ttatatctga ttggatacct gttttagaaa gcagtcattt tgaatatgaa
    12781 tttgatatac cagaaataga gtacttctta cgtatagacg caaatgcata tacccgtatc
    12841 attaataacc tattacaaaa tattattacc catagtagtg gaaacaaaat gacattgaga
    12901 atttttgaaa acaaagagca agctcaaatt ataataacag ataatgggaa aggtatatct
    12961 tcagacaatc tcccacatat ttttgaaagg ttgtaccaat gcgaccattc acgagcttca
    13021 aagggaaatg gtttaggctt ggcgattgca aaggaactta ttaacgctca taagggaaat
    13081 attaccgcca acagcactcc cggtatggga acagaattta caatattgct tcctaaagcc
    13141 ctgtaaaagt acgaggcttt cttactctaa aacaagataa aagcaagatt atggcaaggt
    13201 tttggcaagg ttcatgtttt ataattctaa ttgtagttga ttttgacgtt gtatcaacta
    13261 tcttttgtag aaacaaattt attaaaagtg aggaaattat atatgaatga ttttgtgatt
    13321 gaaacaaagc aacttacaaa aatttatggg aatcaaacag tagttgacaa agtcaatctc
    13381 catgtaaaaa aaggccgaat atatggtctg ttaggacgca acggagctgg gaaaaccaca
    13441 attatgaaaa tgattttagg attgacttca atcacgtatg gcgaggtaga tgtatttgga
    13501 gagaatatta agggacgtga aaaacaagta tatccccgta tcggtgcaat tatcgaaacg
    13561 ccaggatttt atcctaatct gacaggaacg gagaacttgg agatttttgc aaaactgcgt
    13621 ggtacagccg cccctaattc tgtaaaaaga gcattggaaa ttgtaggctt accttataaa
    13681 aataaaaagc tgtttagtaa atattcactt ggcatgaaac agcgacttgg tattgccaac
    13741 gctatcctgc atgacccaga actgttagtt ttagatgagc caaccaatgg tcttgaccct
    13801 attggtattg cggagatgag aaagtttatt agggacttga gcgttgagca tggaaagaca
    13861 attcttattt ccagtcatat tctttcagaa atctcattgt tggctgatga tattggaatt
    13921 atcgaccacg gtgttctttt ggaagaaagt agtatgagtg aactagaaaa gaaaaacagt
    13981 aaatatatcc tgctacaagt ttcagatatt ccgaagacat cacttatttt agagcgtcaa
    14041 tttaacacga aggattattc cgttcaagat gactacacgt tacaccttta taacactaat
    14101 ctggatatgg gtgaaatcaa taaagcactt gtaatgcaga atgtaactgt tattagttct
    14161 caactttgta atgatacttt agaggactac tttaaaaaaa tcacaggagg agagggcatt
    14221 gcttaaactt attcaaacag aatttcttaa actgcgtcgt aggaaactta tttggctgat
    14281 gttgttgtct gctctcatta tgccgttttt cgcactacta tacttcaatt attttggtaa
    14341 aacaggtgtt gagccaataa agttttataa atggtctgca tttggttata cactttggat
    14401 tattttgcct gttgttttgg gtatattttg caccatgctt atacacgaag aaaaccagta
    14461 tgatatgctc aaacagcttt ggattgtacc tattagtaaa atgggatact tttttagtaa
    14521 atttttcgtt gttttgattt actctatttg ttttatgctg attacagccg ttgcttctgt
    14581 gttatttagt gtactttctg gatatgtcgt atttagctgg gcaagcattt tatatttgtt
    14641 gaaaaaatgt ttagaaattg gtgtaatcac tgcttttgta atgttgccga ttttggcaat
    14701 cgctacatct caaaaaggat acattctccc aatttgtatc acattggtat atgccttttc
    14761 cggttttgtt ataatgacaa taaatatgta tttacaccca ttatccagta tgggagttat
    14821 tatcatgaga aataaagaca ttccaggtct tatattttca caggaaatta atgtcccatt
    14881 agcatttctt tgtatttgta tttggagtat tgtatcagta ttgtttgcaa atattgcact
    14941 aggaagaaga aagtgaggtg tagataatgt ggaaattact ttgggctgaa tgtcaaaaaa
    15001 ttcgtcgttc aaaaattgtt tggattacag tttttgcatc cattatgata gcagtcatag
    15061 tttttgcagg ggggcaggat gtatataatg acccagattt acattatggc ttaaagagcg
    15121 tttatgacgg ttcacggtat atagacaatg caggttggta tatggacgca gtacaacctt
    15181 ggtcaacttt ttttgttctt cctgctgtca ttgccctatt aggaagctat attatttgtc
    15241 gtgaagaaga ggaggacact attaaatccc tgcgtcttat tcccataaat gaaacaaatt
    15301 taaccatagc aaaaatgatt attactttta tattcagtat acttctttat ttgttactct
    15361 ttactataac atttttgact gaatccgttt tacatttttc cgatttatcg acaaaattgg
    15421 ttttaagttg catgaaagag tattttcttg atggcatagg tgtattcttt gcaatttctc
    15481 ccattattgc cttggtatcg cgcatgaaaa aaggatattg gcttgcatta gtattcacag
    15541 aagtttattc tattgctgga ctatttgcgg gtatgtcaaa tgtcttacaa accttttttc
    15601 ctattgcggc aatatttaat atttctggat actatataac tactggagaa aaaattatgg
    15661 ggagtatcat gagtttatta ctttgtgcct gcctttctgc atttatactt aaagggctaa
    15721 aacacaatga aaaaaattga aataggtata tcaaaatgaa aaagataaaa aaactgatta
    15781 ttctaattgt attcttgttt ctttttggca tgttacactg ttatactgaa tggaaattca
    15841 acaataaccc acctaaaggg gtaatagaaa gaataaatga gtggactgat aagattggac
    15901 tggattagta acttatgtta attatagtga atgtaatatt agcaagttat tgtttcgtat
    15961 tttaatacta taactttaaa tatttggttt taatcttcca aacgaatata aaaaaactgt
    16021 cacagaggca acatattttt gaatatttat aaagtggtag ttttgatttt caaagctacc
    16081 gccttttctt ctatataagc aaacttatcc tcaacaaagt gtaaaacatg gtgcatttta
    16141 agaaaaaagt accacagaca caagcaataa aagaaacgcc gagaacctac aaaaacgtag
    16201 tgcttatgca gatttacgac gccttctatt ttctcaaccg tcttaatgac cttgtacaaa
    16261 agaaattttt ctaatatttt ttataatctt atcttgtgat gttttttatg tataaatgac
    16321 ttaataacaa atccaataaa aattatggat attggtataa ttagaacgag gaatttataa
    16381 tcattataat gtgacattat tcttacccaa ctctcagaaa aaatatatcc tatactgatt
    16441 aatgaagtat tccatatggt tattcccacg atagatgata ttacaaaagt tataatattt
    16501 tgacctgcta tacctgctat aaaagatatg tatgttctac ataaagggat taatctacat
    16561 acacaaacag aaatagaaga gtatttatta aaataatctt gagatgcaaa tattcctttc
    16621 tttgcctttg gacatatctc tattatttta tttatgatag atttacctcc ccaatttccc
    16681 actgtataac aaaatatcga accaagtata cctgcaatta tacttaaaat taaaattgtc
    16741 agaaatccaa aatgatttct tgcagcaatt gctccacata aaggtaaaac aacttcgcta
    16801 ggtaatggaa aacaagcata ctcaatcata attaatacaa atacagagat taatccatat
    16861 tgtaaaataa aactattgat tacagacact tacttatcta tctcctttct tatttaaagg
    16921 ttttaagggt acatgttcta aggattctca ccctatattt aaagaatcaa tctctttaaa
    16981 ctattatatg tatttacaca aagcatttat aacatacttt aaaaatagta ccttaaaata
    17041 agaaacttaa taaataaaaa aagattacgt ggctacgtcc tactctccca ggcagtttcc
    17101 caccaagtac catcagcgct agagagctta acttctgtgt tcggaatggg aacaggtgta
    17161 tcctctctgc tattgtaacc acataatctt tatgcttaat atttccatta agtttttaat
    17221 aagtttatag aacctagtaa tactagtttc tttagagtta
//
LOCUS       DAEMGG010000086        17210 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEMGG010000086 DAEMGG010000000
VERSION     DAEMGG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17210
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.086"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..1033
                     /locus_tag="KPK24_002567"
     CDS             167..1033
                     /locus_tag="KPK24_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG2300233.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSSELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRIKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            1146..1550
                     /locus_tag="KPK24_002568"
     CDS             1146..1550
                     /locus_tag="KPK24_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG2300234.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            1717..2166
                     /locus_tag="KPK24_002569"
     CDS             1717..2166
                     /locus_tag="KPK24_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2300235.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2322..2699)
                     /locus_tag="KPK24_002570"
     CDS             complement(2322..2699)
                     /locus_tag="KPK24_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2300236.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            2961..3878
                     /locus_tag="KPK24_002571"
     CDS             2961..3878
                     /locus_tag="KPK24_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG2300237.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            4362..5780
                     /locus_tag="KPK24_002572"
     CDS             4362..5780
                     /locus_tag="KPK24_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG2300238.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            5856..6668
                     /locus_tag="KPK24_002573"
     CDS             5856..6668
                     /locus_tag="KPK24_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2300239.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            7091..7708
                     /locus_tag="KPK24_002574"
     CDS             7091..7708
                     /locus_tag="KPK24_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG2300240.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(7865..8317)
                     /locus_tag="KPK24_002575"
     CDS             complement(7865..8317)
                     /locus_tag="KPK24_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300241.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRIFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            8765..10123
                     /locus_tag="KPK24_002576"
     CDS             8765..10123
                     /locus_tag="KPK24_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2300242.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDGEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGLLRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDIRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            10396..10776
                     /locus_tag="KPK24_002577"
     CDS             10396..10776
                     /locus_tag="KPK24_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2300243.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            10901..11101
                     /locus_tag="KPK24_002578"
     CDS             10901..11101
                     /locus_tag="KPK24_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300244.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            11297..12025
                     /locus_tag="KPK24_002579"
     CDS             11297..12025
                     /locus_tag="KPK24_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2300245.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            12047..12964
                     /locus_tag="KPK24_002580"
     CDS             12047..12964
                     /locus_tag="KPK24_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG2300246.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            13106..14290
                     /locus_tag="KPK24_002581"
     CDS             13106..14290
                     /locus_tag="KPK24_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2300247.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            14397..14585
                     /locus_tag="KPK24_002582"
     CDS             14397..14585
                     /locus_tag="KPK24_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG2300248.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            14734..15384
                     /locus_tag="KPK24_002583"
     CDS             14734..15384
                     /locus_tag="KPK24_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2300249.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            15487..16425
                     /locus_tag="KPK24_002584"
     CDS             15487..16425
                     /locus_tag="KPK24_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG2300250.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            16451..>17210
                     /locus_tag="KPK24_002585"
     CDS             16451..>17210
                     /locus_tag="KPK24_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2300251.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDSIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGN"
BASE COUNT         6858 a         1714 c         3000 g         5638 t
ORIGIN      
        1 ttagtataaa ggtattgtta caagtataaa gaaacttatg tatgctattg gaataaataa
       61 taccttaaat tataataaag aataagaact gttaaataag ggacttgatt atcatttagt
      121 tatagataat ataatattaa aaataaagtt taagaggaga aaaaatatga tagatataca
      181 taaaaatata tatgataata agttatttga ggagctaaag attgactgta agaagtgctt
      241 tggactttgc tgtgtagctc tttatttttc tgcttcagat ggatttccta tcgataagga
      301 atcaggaaaa ccctgtatta atttacaacc agattttaaa tgttctgtac ataatagtct
      361 tatgaagcga ggttttaaag ggtgtactgc atatgattgt tttggttcag gtcaaaaagt
      421 agcacaagta acatataagg ggatagactg gatgcaatca tcagaactaa caaatcaaat
      481 gtctgaagtg tttttaatta tgagacaatt acatgaaatg ttatggtatc ttaaagaagc
      541 atctgtatta aatatatcag atacaatcaa atcaaaaatt gatttaataa ttgaagaaac
      601 tgaaaagata actaatatga gcccagaaca gataattaat ttggatatca tatcacatag
      661 aataaaggtt aatttattat tatcacaagc gagtgagtct gtgatgggaa aggttaaatc
      721 atttatcaag acttctactc ttaaaaacat gaaaaaatta agtaaaaata tagatttaat
      781 aggagcagac cttagaaaaa taaatctaat aggagcagat ttaagaggta gatttctaat
      841 agcagcaaat ttaagaaata cagatttgag tggggcaaat ttaataggag cagacttaag
      901 agattgtgat attagaggtg ctaatcttga aaatagtata tttttaacac aattacaagt
      961 aaatactgca aaaggagatt ctagtacgaa gttaccagct tcactaatac gccctaaata
     1021 ctgggaaaaa taattaaatt aatacataat tttacaaaac atgaactttt ttgactttaa
     1081 ttcatattat acattagaga ttctttacaa gaatttctta atataatata gggagagata
     1141 taaaaatgag ttatcaaaat tataaatgtg aaagagatta tgtgtatgat gattataaat
     1201 acaataagtg caacaagtgc tataatgatt atgaggaaat gacacatgtt catgaatatt
     1261 cagaaagtgt taagttagca gaagaatgtg aggatagaca taaccaccgt gcagcagggg
     1321 ttactggtga agctatacca ataaatggag gtacaaatca tgttcataaa ataaatgata
     1381 atgtagattt ccttgaccat ttccataaga tatgtgttac aacagggcca gcaattagaa
     1441 tacctggaac tgataaacac attcacttaa tatgtggaga aactactgtt aatgatggtc
     1501 attgtcataa attccttttt actactcaaa tagaagcacc tttagtataa gctgatgtga
     1561 attattgtag tgaatttgct agattatagt ataataatat ataatagata atatttaaac
     1621 ttaatgagga ctatgatatt taaccttcgt agtcctttct aagttttgta taaaaaagtg
     1681 ttatgcaatt aaaagtgata tagggaggaa atttttatga aatctggaat aattcttgaa
     1741 atgaaacaaa taaattcaac agaatatgac gaaatgttgc aagtctggga atcatcagta
     1801 agagcaacgc atgatttttt aacagaaaaa gatattgaat ctttgatacc attagttgag
     1861 attggactta aagaagtaga aaatatagtt tgtataaaag ataatgatat aataaagggc
     1921 tttataggaa tagataaaga taaaattgag atgctcttta tagaagataa atatagagga
     1981 aatggtattg gtaaaaaatt aataaaatat gctatagata aatacaatgt aaaatatgtt
     2041 gatgtaaatg agcagaataa aaaagctgta ggattttata ttcacttagg atttaaagtg
     2101 tttgataggt ctgaaataga tggacaaggt aacccatttc caatactaca tatgaaatta
     2161 atttaaagtt atacttctca tttattcgca aaaatattta cttaaatttt tatacaaata
     2221 aaagtcgtga cagtcttagg acacgacttt tttactacac tattaaattg aaatttatct
     2281 gttctatatt atgattatta gatgtcgttt ttttatttta actatgtact taaataccaa
     2341 gtatacccat acctatcaag tacttcagca gcgccatcat tttgatgtgg tggtgggtgt
     2401 ggtaaaaatt cttgtacaag tgtaccacct tcggcaagtt ttacagatat ttcttctatc
     2461 gcttctcgag atggtagaac aacacaaatc gccatgccag aaattacaac aggcttgtct
     2521 gtatccgcac ccattataca accttcgtat tttgtgaaag taacacttgc gtgcataata
     2581 ggattgtttg gattgctgtc taatcgcgca aatatagttt ttcctcctcc gaaagcctca
     2641 gcataaaaat taaatgcttt ttcacaagta ccattaaata ttaaatatgg agttagcatg
     2701 accaatatat cctccttgat atagaataat ttatagttgc acctgataat ctaaattatg
     2761 atttagtaaa ttgtatcata gggaaacctt gaagtatata attttttatt attatattat
     2821 acaaatttat taatttaaaa aaatgttata tgtacggaag gtttacagga taaaatataa
     2881 taaaaagatt aaggtgacat tataaaaaat gtatattgac attgacgcta cgtaattgta
     2941 tatactttgt cttaaaggag atggtaaata tgaatattat agaaattcgc tcagataaaa
     3001 tatacaagaa gataatggat gcaccaataa acaaaaaaga agatatatac agatatgaat
     3061 tgatgaagcc ttttgaattt aagtggaagt gtatgaatgt tccaatagtt gctagacaga
     3121 aaggtggata tgatgtaatt atagcaagtg aaatgttagg ggttttatcg cctaaggata
     3181 ttgatgaaaa acaaaaaaag aatataaatg tgttatctgc tgataaaatt tgggccactt
     3241 gtaaagaaac catagaaaac tctataaatg cttttataaa agaagggtat gatttaaata
     3301 ttaaggacta taaatattca atattattgg cgaatccaaa tagtccttat acaatattaa
     3361 gtgatggata ctggggtgat ggtgggattc ctggatatat atttctatca ttggttccta
     3421 atgaatatac tatcaataga ttaccagtat taatagcaca tgaatgtaat cacaatatta
     3481 gatttcagtt tatagagtgg aataataata taacattaga agaaatgatg ataaatgaag
     3541 gtcttgcaga aaattttgca acatggatgt ttggagagga aatgttagga ccttgggtca
     3601 gtagaacaga tatcgaaaca ttaaatactt atataaagcc aataataaaa agtgctttaa
     3661 aagaaactgg atttcaaaat ataacatctt atctttatgg tgatgatata gctaaaatgc
     3721 aaggatattt tccagtaggg ttgccttatt gtgcaggata tgcttgtgga tattatatga
     3781 ttaagtatta tttagaaaag acaaataaat caataatcga agcgacttta ttgccttata
     3841 gtgagataat cgaagcagta aaagagtttt gggaataaat tttataaatt gcataatttg
     3901 aaataaatta tgcaattatt ttatttttaa gaaaaaataa attgacaata atttataaga
     3961 aatatacaat aatatatata aagaaatatt tcaatattta aaagttttaa gatttcatat
     4021 ctagagtacc taacattaca tgttagtttc ttgttactta tctaattact ttatatttcg
     4081 tagttatata taaaattact ataaaaaatg aaatatatta aacaatcaac taaaaaatga
     4141 aatattaata aaaaaaaatt caaattataa atattaaatg aatataatta aaacaaatat
     4201 ataaatttaa gtataacact cacattttaa tgtattgtga aatttttgtt gaactttatg
     4261 taccataact tataaaattt atgtcatgtt atatttgaat aagtaataaa attgtagatg
     4321 taaattaatc tacaaaattt atatgaaatg gagttgatgc tatgacagaa ttaatgatta
     4381 taagtgggtt agtactattg atttgtataa cttctagcaa ggtattgtat aaatttggag
     4441 taccaatatt attgattttt atcttactag ggatgttatt tgggtctgat gggatggtag
     4501 gaatctattt tgataactat gaactgacaa aacaattatg ttctattgga ctaatattta
     4561 taatgttttt tggagggttt ggaacaaact ggaatatggc taaacctgtt gctatacctt
     4621 ctatattaat gtcttcactg ggtgtaataa ttacggctgg ggttacaggt atgttttgcc
     4681 acatggtaat aggaactagt ttacttgaag gtttattaat tggggcaatt gcagcttcaa
     4741 cagatgctgc atctgtgttt gctgtgttgc gttctcaaaa actaaactta aagggttcac
     4801 ttgcatcatt attagaagtt gaaagtggaa gtaatgaccc aatagcgtat atgttgacac
     4861 taattctttt gacattgatg aataattctg gggtagaact tattgcacca atggtaataa
     4921 aacaaatagc ttttgggtta gctataggat ttatactagc aaaattatct gtttatattt
     4981 taagacattc aaacttcgag atagaaggat tttatactat atttataaca gctattgcaa
     5041 tactttctta tgcatttagc gagtatcttg gaggaaatgg atatttaagt gtttacattt
     5101 caggtattat tataggaaat agtaggatac ctcttaaaaa aagtttggtt catttctttg
     5161 atggaatttc atggattatg caaataatgt tattctttat gttaggattg ttatctttcc
     5221 cttctaagtt accaaatgta tttggtatag caatggccat atctatattc atgatagtta
     5281 ttgcaagacc attagcaaca tttatagttc tagcaaaatt taaattctca aataaagaaa
     5341 aattattcat atcgtgggta ggtcttagag gtgcggcttc agtagttttt gcaatttatg
     5401 ctgttactca aggagtagcc atagagaatg acatattcca tataatattt tttatagcgt
     5461 tgctttcagt aggaatacaa ggttcattga taccaactgt agccagaaag ttggatttag
     5521 ttgatgataa tacacctgta ttaaaaacat ttaatgatta tgatggagaa attagctcta
     5581 aacttatcga agtaaatgtt gatgagggta ataaatgggc taataagagt ataatagatt
     5641 ctaatatacc agacgatata ttaatagtta tgataaaaag aaaggaacag gttttaatac
     5701 ctaaaggttc aaccattata aaaacagggg atattttggt attgagtgga gatagaattg
     5761 aagaattaat acaattataa tttattttgt attgacttta acctaagggg aaagtttaaa
     5821 cttaaattaa tcaaatctaa tgcgcaggtg atgagatgtt ttctatagga atgttttcta
     5881 aaataaataa aataactaca aagacattaa ggtattatga agatataggg ttgttaaaac
     5941 cagaatatgt agatgaattt acaaaatata ggtattatac aacagagcaa ttacctaaat
     6001 tacatcagat aataacttta aaacaaatgg gtttatcatt agctgagata aaaaaagctg
     6061 tagaaaatcc tgaagaagtt gaaaatattt taatgaataa agaaaaagaa atgttggatg
     6121 taattaaaag tgaagaatat aaaataatta aactacgtaa ttaccttatg aatttgaaag
     6181 gagaagtcaa tatgaatgat gtaattatta agtctttacc aaaagttaaa gttgcttcta
     6241 tgagaaaagt gttaaagggg tatgatgaac tttttaatct atttccaaat gtaatggctg
     6301 aagaaatgat gaaaacaggg tgtacatgtg cagttcctga ttattgtttt aatatatatc
     6361 atgatggtga atatagagag actgatatgg atgttgaaat gtgtcaagct ataaatgatt
     6421 taaaggaaga tacggatata ttaaaattta aagaattaga tgcagttgaa aatgcagttt
     6481 gtatattaca caatggtcca tattcaacat taggaaaaac ttacgcagat gcatttaaat
     6541 ggatagagga caataaatat aaagtggttg gaaatcctag agaatcttat atagatggta
     6601 tatggaataa agagagtgaa gaagactggt tgacagaagt tcagatacca gtgaaacgca
     6661 tgttataata tgtctacaaa gtataaataa gaataaaaaa ggtggtgtct caaagtaaac
     6721 attttagttt atgagacact ctttttcata tgaaatcaag tatattttta taattacgat
     6781 tatgaaaaaa ggcttatcta tatttttaga cagcctctta tttttttatt ataatagttt
     6841 tctaaagatg ctctatttat aaatttttat taaagacttt ataaataatt gtatttaata
     6901 tacaaaatat atataaatat tgatgtttta tatataaagt tgtatatttt tttaaggata
     6961 taaagtaaaa aaatatacaa tatcaagaaa aaaagatatt gggtatttaa agtaatactt
     7021 tatttaattt taagtaaatc gggtatctac atatatagat actaatttca aagaatagga
     7081 gggtgtttat atggaaatat tcgcaatagg acattcaaat tatccatatg ataagcttat
     7141 taatatgata aaaaaatatg acataaattg tgtagttgac attagagaaa ctccatattc
     7201 taaatataat attcaataca ataaagaggc atttaacgaa tctcttagaa attccggatt
     7261 tttatatata tacatgggaa aagaatttgg agctaaaaga actaataaag atgtatatac
     7321 gcaagaaggt tatgcagatt ttgagaaagt agcaaaagaa gatatttttt taaacggaat
     7381 agaaagatta aaaaagggtt gtcaaatggg ttataggata gttttattag gagctatgca
     7441 agaacctata agatgtcata gaagtatctt ggtgggaaaa gtattaaata aagaaggttt
     7501 tgatgtaaaa tatataatgc atgaaggaaa tttggcatat caggaagata tagaggaatc
     7561 attattggat aagtatttta gtgatagaaa gcaactgagt attgacaatt tattagggag
     7621 tgcattaacg agagaagaaa tgatacaaga aggttataag ctagctaata aagaaatagg
     7681 atatagaact gagaaactag gtaaataaaa atatacaagt agataaaata cagtacttat
     7741 cttaagtaaa ataatattat gaatttataa tagctaggca tgatgtgcca attaaaaata
     7801 ttctagttat ttaataatat cattaaattt agggtatttt ttaatagcac atcatgttgc
     7861 agttttaaaa aataaaatct ctaatattgc cataatcaac tgttctatta agagtaggat
     7921 gactagatat aaatctaact gtaatttttg aatttaacaa acaaggattt gaaattaaaa
     7981 atattctatc tccattgaca atagaagtaa tagctactgg caaaatatct acaaatgtat
     8041 catatctgtt tttagcaatt tgataaggat aatttcctac aaaaacaaat agatttaaat
     8101 catatatatt atcagtaaac caagcccatt gaccataaac tttgtcgata ttcattgtgc
     8161 ctaaaccaag tgtatcaaag tatgttgcta tagttaaata aatattttcc atgttttcta
     8221 aatatgaaac agtatatctt ctgaaacgta tgggtgctgt ggaagaaata ggataaacca
     8281 tatttacatt tagattattt gcatttaaat taaccaaagt atcacatcct aaaattagtc
     8341 tataaaatac aatatttaaa tggatgcgat tttgttacat ctaaagtgta atattttata
     8401 tattgaaaaa gtggtaaaag tttggatgat taaataatat tataaaaaat atataaaatg
     8461 tacttattgt tattgctaca cgatagtgat acaatattaa cgaaatatag ctacttttaa
     8521 taataacgga aaatatttat attaaattaa taaatatttt aaatgtggta tccataaaaa
     8581 ataataaaca taagataatt ataaagatat actgtgttca aatgatatta aacaagattt
     8641 atattatttg aaagcagtat attataatgc gaacaataca attctaaact ttgcattgtc
     8701 aatttacagt tcgataaact aattaaaaat taaatttata taaagaggga aaagaggaat
     8761 agggatgttt ataaggaaaa atgttataaa gttagcaatt cctataatga tagagcagac
     8821 gtttgtcatg cttttaggag tatttaatac aatgatggca ggtcatattg gggaagaagc
     8881 tgtatctgca gttggaatgg ttgattcaat aaacaatata tttatatctg tatttgctgc
     8941 actatctgtt ggagctactg ttgttgtagc tcaacaaata ggaaaaaaag atggtgaaaa
     9001 ggtgaatgaa actattaagc aggctctagt gtctggagta atattatcct ttacaataac
     9061 aatattgatg tgggttttta gaactggtgt aattaattta ttttatggtt ccgcagaaga
     9121 attggtaaaa tcaaatgcaa aattatacat agagtttaca ttatttacgt atccatttat
     9181 tgcgacacaa caaatagcaa atggtctttt aagagggtgt ggagatgtaa agaaacctat
     9241 gtatgtgact atgtttatga atatagtaaa tgtaacatta ggatatatac ttatatatgg
     9301 tattgattta aatcctctag gaataaattt gcaaacacca tcctatggca taacaggtgc
     9361 agctatatct attgctatag ctagaataat tggatgtata gtaattataa gagctttatt
     9421 taaaggaaac gaatttatat ctatgggtaa aatatttcca tttaagattg atgttgaaac
     9481 tcaaaagtct atatttaata taggaatacc agctggagtt gaacaactac tttttaatgc
     9541 aggtaaattg attgttcaag tatttatagt tactatggga acatcagcaa ttgcatccaa
     9601 tgcaatagga atgtcaattg catccataat aaatgtaatt gggaatgctt tagctttgtc
     9661 tgctacaacc ttggtgggac agtatatagg acgtggagat attaaaggag ctaagagtac
     9721 tttattatat ttaactaaat ttgcaacaat ttgtttattt atagtaggag ctatatttat
     9781 accaattgca ccttggattt caagtatgta tactcaaaat gctagtgtaa ttgacatctc
     9841 aacacaatta attcgaagtg attgtttagc tatggtaata tggcctatat cctttgtatt
     9901 atcagcaggg ctaaagggag ccggtgatat tagatataca atgatgactg cagtaatagg
     9961 tatgtggatt tttagaattt ttacaggtta cgtattgggg gttgtattag aatttggagt
    10021 gttaggaatt tggataggta tgtatactga ttggcttgtt agagggactt tatattgttt
    10081 aagattaaga ggaacaaagt ggctaaaaca caaagttgca tagaatatat gaaatcactt
    10141 ttagatggat aactctattt agaagtgatt ttttatgtag ttattatcat aattataagt
    10201 atgatatata aggtgattaa attacataaa taaaggctat aatagtatgg atattataaa
    10261 ttatatattg agcagaatta cataatttgg cgataaatat tagaaatata tatctataga
    10321 aaataatata tatgggtata ttattaaata taaaaaatat taggtattta tttaataata
    10381 aaggtgggta ttgttatgat aaataaaata ggaaaaataa ctctctatgt aaataatcaa
    10441 gaggatgcaa agttattttg gacagaaaaa ttgaactttg tagtaactta tgaaaaggaa
    10501 atgggtccaa atatgaaatg ggtggaagta gcaccaagta aaaatgaatt tacaacattt
    10561 gtgttgtatg ataaaaatct aatgatgaag caaaacaaag agataaatgt atctcatcca
    10621 tcaataattt taagtacaag agatattaat ggaacttatg aacaaatggc aaataacgag
    10681 gttgaggttg gggaattaat ggagatgcca tatggaagga tgttttcttt taaagaccaa
    10741 gatggaaatg attatttggt cagagaggat aaataatttt acaaagtatt aaatttactc
    10801 aatatctgat ataattagag acaataatta ttataaacta gtctaggatt agaattatat
    10861 aattaaaaat taagtgtaaa ttttgagtag aggtgataaa atgtttaaga aaatggcagt
    10921 actaaaagat atagcaacta aaataggtcg taaaaaagcg tatgaactat tagaaatggt
    10981 tgaaggtaat gatgcctttg tagctgaggt aaagataaaa aagaatggaa tagaatctaa
    11041 aaaagaagaa attatgttaa aagataatca aaaaataata ttagagtata tagaaggtta
    11101 aattttaaat acatataagt tgatatacta aatttctgag atgagtttac ataaaactca
    11161 tcttttttac ttttcaataa ctttgtttta ttatttaata tacattttat ctccttagag
    11221 ttgctgatat ataaaaatta ctgatataat aaaagaatgg atattaatgt aatattttat
    11281 agaaaagagg ggtattatgc ttgaaataaa aaatctttcc tttaatgttg agtctaacaa
    11341 tgaggaacta ggaataataa atgatgttag cctttcattt gagaggggaa aacttattgt
    11401 tatcacaggt ccaaatggtg gaggaaaatc tacaattgct aagcttatta tgggaattga
    11461 aaaagctacg agtggtcaga taattttaga tggagaagat ataacaaatc ttagtattac
    11521 agaaagagca aaaaaaggaa taggatacgc atttcaacaa ccaccaagaa taaaaggtat
    11581 gacagttgaa aatttactta cacttgctca tggaaaacca ttgtctactg atgtatgttg
    11641 ccaatacctt acagatgttg gattgtgttc aaaagactat ttaaatcgtg aagttgataa
    11701 ctctctttct ggaggggaaa tgaaaagaat tgagatagca actctttttg ctagagattt
    11761 aaaggtttct atatttgatg aacctgaagc agggatagac ctttggagct ttggaaaact
    11821 caatgaaagt tttaagaaaa tacatgaaga gtctaatcag actataatca taatttctca
    11881 ccaagaaaga atattagagt tagctgatga aataattgtt ctacaagatg gttcagtaaa
    11941 aagccatgga acaaaagagg ctatattacc tgaaatcatg tgtcaagtta attcaagttg
    12001 tgaattaatg aaagacatga attaattaga aaggaaggca aattaaatga acaaaataga
    12061 tttaaatctt ttagaaaaag ttgctgaact tcgtggaact ccattaggag catacaatat
    12121 acgtaaaaat gggcaaggag tagagagaca ttcaactgaa aatgtaacta taattcctaa
    12181 aactgataaa cctggaatag atattatagt aaagccaaat acaaaaggag aaaatgtaca
    12241 tataccagta atagtaacag aggctggtgt acaggatatg gtatataatg attttgaaat
    12301 tggagaaaat gcagatgttg ttataattgc tgggtgtggt atacataact gtggttgtga
    12361 tgatagtgaa cataatggta tacatagatt tttcttacgt aaaggagctc gcctaaaata
    12421 tgtagaaaaa cattatggtg aaggtgatgg agaaggaaag agaattttaa atccagttac
    12481 tattgtcaat atggatgagg attcatattg tgaaatggaa acaattcaaa ttaagggtgt
    12541 agattcaact aaaagagaaa tggatgcaaa cctaggtaaa ggagctaaaa tagttgtatt
    12601 tgagaaatta ttaactcatg gtaatcaagt tgcagaatct aatatgaagg ttatcttaga
    12661 gggtgaaaac tcttctgctc aagtagtttc tcgtacagtt gggcaagata attctaagca
    12721 aaagtttaat ccttgtgtta ttggggattc cctttgttct gcacatgttc aatgtgattc
    12781 aataattatg ggaaattctc aaattagtgc tatacctgaa attgctgcaa atcatccaga
    12841 tgctctttta gtacatgaag cagctattgg aaagattgca ggagaccaaa tactaaagat
    12901 gatgacatta ggtcttactg aagaagaagc cgagcaacaa atacttaatt gcttcttaaa
    12961 gtaaattaaa gattttattt aatttaatat aattaatata gtataatata gtgaataagt
    13021 ctaaaatatt tttattttta ggcttatttt ctttatgtaa gaaaagctat tagagaaatt
    13081 atattgtaat taggatgtgg aataaatgaa aaatttactt ataagaaatg ggacaataat
    13141 atctccaaca gatggtatca aatttgaaaa aagagatatc ttggttttac atgggaaaat
    13201 atatgctatt ggattgaacc ttaaagagaa aataagaaaa gaaaaattac ttacaaataa
    13261 agagttcgag ataataaatg ccgataatat gtatatttcc ccaggattta tagatgttca
    13321 cacccattgt tataagagaa aattacctac aggtatggat tctgattcaa ttggaattga
    13381 aaaaggttct acagttatat ttgatgcagg tacagctggt ccttcaaact actatgattt
    13441 taaaaaaaag tatatggatg agtgtaaaac agaagtatat tcttttttaa atgttacaaa
    13501 tgagggacta aatatattga atgaatcaac ttctttagat gttataaatt ttgacaaggt
    13561 tgagtcagtt gtagaaaaaa atatagaaaa gataaagggt ataaaagtaa aagcgagtaa
    13621 atttcaatct aatgaaagtg gtatagatat aattaagaaa tcaaagcaag ttgctaaaaa
    13681 attaaattta cccttagtgg tatatatagg agagtatcca tcatatatag atgaagtaat
    13741 gaatattttg ggaaaaggag atgtggttac accagtatat ggaaatagta caaatggaat
    13801 ccttaaagaa tctggcacat taagaaaatc agtaataaat gcaaagaata gaggagtatt
    13861 gttcgatgta tgtcatggag tggaccaatt tgattttaaa gtttttaaaa aagcaataaa
    13921 gcaaggttta gagcctgacc taataagtac tgatatgcat ataaaaaaca tgaaagacgt
    13981 aaactattct cttttaaatg ttataaataa aattatggaa cttgggatat ccctagaaaa
    14041 gtgtatagaa aaagtaactg aatacccaac aaagattttc gatttacatg gaaataaagg
    14101 cagaataaag attggtggag aaggtgattt tacaatattc acaatggagg agtgtaatga
    14161 tattataaaa gactacaata ataatgaact tgttttaaat aaaaaattaa gattgcaata
    14221 tactgtaaaa tcatggatga aaagcagtga agtttataga catggatacg aaaatactat
    14281 aataaactaa acaatagtga taaaataata aatatatgtt taaattggta tatattggat
    14341 aaatatctaa ggaaacaaca attaaaaaat aataatttta ggggaggtat tttagtatga
    14401 aagcatttgt tgataaggat gtatgtgtag cttgtggagc atgtatagga acatgtccag
    14461 acgtttttag tatgagcgat gaagggcatt cagtagcaat tgaaggagaa ataccagcag
    14521 atttacaaga tttatgtaaa gaagccgaag aaggatgtcc agtttcagct ataacagtta
    14581 aatagagctg tttaaattta tttttaaaat aaatatataa gatttaattg gtacttaagc
    14641 tatagtatta gaaatttcta ttactatagc tttttaaaat agataaactt aatatataag
    14701 atattacttt ttacaaatat ggaggactga aatatgaaaa aaaattatga aatagtatta
    14761 ttcgatttag atggaacact tacagaccct aaagaaggaa taacaaagtc tattcagtat
    14821 tctttaaata gttttggcat aaaagaagat ttggaaaact tagaccaatt tataggacca
    14881 ccattacatg atactttcaa ggagtattat aaatttgaag ataaaaaagc taaagaagct
    14941 gttgaaaagt atagagaata ttttgctgat aaagggatat ttgaaaataa aatttatgag
    15001 aatatgaaag agatattaga aatgctctat aaaaatggta aaatattatt agtagcaact
    15061 tctaagccaa cagtttttgc agaaactata cttagatatt ttgatataga tagatatttt
    15121 aaatatatag caggtagcaa tcttgatggt acaagagtga ataaaaatga agttatacaa
    15181 tatgtactag acctatgtaa tgtaaaagat aaagataaag ttattatggt tggagataga
    15241 aagtatgaca taattggagc taaaaaaata ggtatagatt ctattggagt attgtatggg
    15301 tatggttctt ttgaagagat tagtgaaagt gaacctactt atattgtcga aaatgtagag
    15361 tctataaaag atattttgtt ataaatatct tttatagaca aaaatattac aaaatggtgt
    15421 cgcatattta aaaatatgat attatagtta aggaagtcat ttaaaaaaga ggaggatgca
    15481 cttaaaatga aatctatttt aattatgggt ggaagcgact tcataggttc tgcacttgct
    15541 aagcgcttga ttaaatgtgg gtatcaaatt gatatactta caaatggtaa aaaggaaata
    15601 gattataatg gttttaaaga acatctaatt tgtgatagaa aagttcgaaa agatatggaa
    15661 aacattatca caggaagaaa atatgattac atatatgata tgacagcata tacaaaagaa
    15721 gatgtaagta atctgattga ttttataagc atggataact taaaaaagta tatagtattg
    15781 tcagcaggag cagtatataa agatagtgga agaaatataa aagaagaaaa tgagaaaggt
    15841 gaaaatgaaa actggggaaa atatgggtta aataaaaaag aggctgaaga ttttattata
    15901 aatagcccta taccatatat aattattaga ccaacttaca tatatggtga aaataataat
    15961 ttatatagag agtattattt ctttgaaaaa atagaaaaaa atgaaaagat accagtacca
    16021 aaaggaaaac aagttagtaa tcaatttata tatattgggg atttggtaaa ggtattggaa
    16081 agcataatga aaaatcctca tgtaagagag gcctataatg tgacaaatcc acagttaatt
    16141 tcttgggatg accttatata tacatgtgga gaaattattg gcaaagaacc tataataaaa
    16201 tatgtagata tggaaaaagt agagtttaga gaaagaacgt atttcccatt tagaaatata
    16261 gactttaatc ttgatataaa taagcttatt gaacatgggc tttatatacc aaacgtgtta
    16321 ctaaaagaag gtcttacagc aacatataaa tggttttcag caaataagcc aaaaatgcat
    16381 gacagaaaaa tgaacaaagt tgagtctgta atgcaaatag tttaaaaaaa taaataagta
    16441 ggtagatatt ttgaactcaa aaattaagta ttttttaaaa ttttttatct taatatttat
    16501 tttgattttt atatactcag ttgcgataga gccgagtcta ttaattgtga aaaaatataa
    16561 tataagtgaa aatggagcat tgataaaaaa tgattcaatt gagagtaaat ctgaaagtgt
    16621 aaaagtagtt caaatttcag atactcaaat aggtagtttt tattcaacta agaatctcaa
    16681 aaaagtagct aataagatta acactttgaa tccagatata atagtattta ctggtgattt
    16741 aattgattac tccaataaaa atccaagtgt agatgaaatt acaactattt tatcaagtat
    16801 gaatgctagg ttgggaaaat tttcagtatt tggcaatcat gattatatgt ataaacttcc
    16861 taggtattat aggcagatta tgaaaaattc caacttcaat cttttagtaa atgaaaataa
    16921 aaaaataaaa ttaaaagatg ataaatatat taatatatta ggtgttgatg aaatattaaa
    16981 tggaaatcct aatataaaat atctggagag tcaaatagat aataaaagtt ttaatttatt
    17041 gttagcacat gaaccagatt tagtagatat gcttagtaaa gatacaatga atttagttct
    17101 atctgggcat agtcatggtg gtcaaataag attacctatt aagggagcat tagttactcc
    17161 tccatatgga aggaaatata ctaaagggtt ttatgatata aatggaaatc
//
LOCUS       DAEMGG010000087        16776 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEMGG010000087 DAEMGG010000000
VERSION     DAEMGG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.087"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            26..898
                     /locus_tag="KPK24_002586"
     CDS             26..898
                     /locus_tag="KPK24_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG2300252.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(1095..2282)
                     /locus_tag="KPK24_002587"
     CDS             complement(1095..2282)
                     /locus_tag="KPK24_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2300253.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(2287..3357)
                     /locus_tag="KPK24_002588"
     CDS             complement(2287..3357)
                     /locus_tag="KPK24_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2300254.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            complement(3367..4689)
                     /locus_tag="KPK24_002589"
     CDS             complement(3367..4689)
                     /locus_tag="KPK24_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2300255.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            complement(4721..5056)
                     /locus_tag="KPK24_002590"
     CDS             complement(4721..5056)
                     /locus_tag="KPK24_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2300256.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            complement(5153..5458)
                     /locus_tag="KPK24_002591"
     CDS             complement(5153..5458)
                     /locus_tag="KPK24_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2300257.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            complement(5473..6516)
                     /gene="ypdE"
                     /locus_tag="KPK24_002592"
     CDS             complement(5473..6516)
                     /gene="ypdE"
                     /locus_tag="KPK24_002592"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG2300258.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            complement(6519..8420)
                     /locus_tag="KPK24_002593"
     CDS             complement(6519..8420)
                     /locus_tag="KPK24_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2300259.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            complement(8746..9597)
                     /gene="gatY"
                     /locus_tag="KPK24_002594"
     CDS             complement(8746..9597)
                     /gene="gatY"
                     /locus_tag="KPK24_002594"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG2300260.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(9646..10815)
                     /locus_tag="KPK24_002595"
     CDS             complement(9646..10815)
                     /locus_tag="KPK24_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2300261.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIVEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            11285..12202
                     /locus_tag="KPK24_002596"
     CDS             11285..12202
                     /locus_tag="KPK24_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG2300262.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMQFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAISGEECIKAQRLLEKIINSVK"
     gene            complement(12331..13260)
                     /gene="pfkB"
                     /locus_tag="KPK24_002597"
     CDS             complement(12331..13260)
                     /gene="pfkB"
                     /locus_tag="KPK24_002597"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG2300263.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVSK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYYVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            13527..14252
                     /locus_tag="KPK24_002598"
     CDS             13527..14252
                     /locus_tag="KPK24_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2300264.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            14335..15489
                     /gene="nagA"
                     /locus_tag="KPK24_002599"
     CDS             14335..15489
                     /gene="nagA"
                     /locus_tag="KPK24_002599"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG2300265.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(16188..16670)
                     /locus_tag="KPK24_002600"
     CDS             complement(16188..16670)
                     /locus_tag="KPK24_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2300266.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF
                     "
BASE COUNT         5681 a         2681 c         1977 g         6437 t
ORIGIN      
        1 taaaatataa taaaggagat tttaaatgaa tatattacaa aatgaaaatt taattatcga
       61 atcaagcagt tttggtgctg aactaactaa aatattttct aaaaaatacg acaaaaacat
      121 tctttgggat ggtaataaaa aatattgggg tagacaatca cctatattgt tccctatagt
      181 aggtaaactt tttgataatg aaacaataat agaagaaaat ttatatagca tgactcaaca
      241 tgggtttgct agagatatgg actttgagat tgtagataaa ggagatacat ttgtaactta
      301 taaattaaca gataatgaat ctactttaaa gaagtatcca tattcttttg agctaatcat
      361 aaactataca ttaaatgata atagtattga ggtagaatgg attgtaaaaa atactgattc
      421 taaagatata ttcttttcta taggtggaca tcctgcattc aaccttcctt tttacgaaca
      481 aaatgatttt tctaaacact accttgagtt taaaagtaaa aatgatgtag aaaaaatcaa
      541 tttaaatggt tcttttacag atgatataat acctattgga aaattaaaaa acttacagtt
      601 aaatccagaa gtattcaaga atgatgcttt gatatacacc aacatagatg aagtatctat
      661 atgtaataat gatggtagta aatatgtaac tatatctatg gaagatttcc ctctagttgg
      721 tatttggact ccttattata gtgaaactaa ttctactgct ccttttttat gtatagaacc
      781 ttggtatggt cttgctgata gtataaattc taacaaaatt tataaagata agaagtttat
      841 taataaactt agtaaaggaa aagtttttac tgcttcttac aatataaata tacactagat
      901 aaaggttagc taacccgttc ccaaaataat aaaatatttt tatataagtt aatataaggg
      961 tatgaaataa tattatttaa tattcctcaa taatattcct taatcaaaaa atattactgg
     1021 aatacaaaat tccagtaata tttgataact aaataatgtg tttttatata aaccatagat
     1081 acttttttta tgatttaaca atacatactt atatacgttt ttttgattct atttagagcc
     1141 tcatttaatg tttctctagg acaccccaca ttaatacgaa taaatccatc tccatctttc
     1201 acaaaataac ttccaactac catagttaag tttgcatgtc taaaaaattc tttagattct
     1261 tcttcatttc taaatacttc tcttacatct atccaggcta aaaaagaaga gtctgacttc
     1321 atcactttag cttttggtat attagtttca aagaaatcat aaactatttc aaaattctcc
     1381 tctacataac tagtcatagc atctagccat tcttcgcttt cattatatgc tgcaactatt
     1441 gctggtattg caaatacatt tggagaagtt attgaattac tttttagtct ttctaataaa
     1501 gtctccctaa tttttacatc tcttattaat acatatgatg tttttaatcc acctaagttg
     1561 aagcctttat taggagatac acagattata ctatttttat aaatttctgg atgagcattc
     1621 cataaagata caaatctata atctttaaat attatatctc tatgaatttc atcacttacc
     1681 aaaattacat tatactttaa acataactct gacacttgat atagttcttc ttttgtccat
     1741 atacgacctg atggattttg tggactacag aatataagca atcttatatt ctcgtatttc
     1801 atttgatttt ccatatcctc taaatctaaa taatatctat tgttatctaa ttttaatgag
     1861 ctacaacaca actttctatt attatttact acagcctctg caaaagggtc ataagcaggt
     1921 gtatttatca atacaccctc tccttcttta gtatatgctt ggactatata atgaagggtt
     1981 gaaacagtac caaaagttaa ttttatccaa tccttttcta tatatacatc aaatcttctt
     2041 ttattccaat ttataatcgc atcatagaag tcatctccta taaaagtata actataatct
     2101 cctactgatg ctctttttaa gatagattct tttatagcag gagcacactc aaaatcgata
     2161 tcagctaaat ccataggaat tgcatcttca tttaagccga atttttcttt aatcacatgg
     2221 ttgctccatt ttctacaatt tccttcttta cgatttaata ctttattaaa attatatttc
     2281 ataattttac tctcctaata ttataaactc tttggatgtc ttattgagtg gaacaccttt
     2341 tccatctatt ataacaatgt catcttctat tctaactccc cctacatttg gaatataaat
     2401 acctggctca caagacatta ccattccttc cactaatata tcttcactat ttggatttaa
     2461 agtggggact tcaccatctc caagacctat accatgaccc agtccatgat taaaatattc
     2521 gccaaatcca tgtttatcta taatttttct agccactttg tcaacatcac ttgccctagt
     2581 tccttccttt attgactcta ttgcagataa ttgtgcttcc aatactacat catatatctc
     2641 tttaataatt ttaggaggtt tacctataga tattgtacga gtcatgtctg attgataccc
     2701 ttgataaaca acaccaaaat ctattgttat agcttcattc actttcaatt ttttgtttgt
     2761 aggtcttcca tgtggcattg cacctctttc tcctgatacc actattgttt caaaagacat
     2821 tccacttgca ccttttttta atgcatgata ctgaattaga gctgatactt ctagctcagt
     2881 catatctttt ttaatttcag aaataacctc atcaaatact tcatctgtaa tttcacaagc
     2941 ctttttaata agttcaattt cttctttaga ttttatagcc cttagcttcc ataattcatt
     3001 tgttattaaa ttgatttcaa aaccttcatt ttttagtgaa agatactctt gtatactcat
     3061 agcttggttt tctattgcta acttttttat atttaattct tttaataact caattatagt
     3121 tggtatataa gaaccttgag atacaatcct ttttatgaaa ccagtcgttt ttttatctgc
     3181 ttcatcaata tatctgccat ctagaatttg ataattttta tccttagtaa caagcacata
     3241 tacaccacta cctgataaag ctcctatata tcttttagta ttttttgctt taatcaaaag
     3301 agcgtcaaca cctcttttat agattaagga tactaatctc tccattgtat tattcataac
     3361 aaaatattag aaatccaatc ctaaatcatc caataagtct tcttcttctt tagagaatat
     3421 attcttagtt tcttcttttt gttttttatt attttgttga cgttccatta ttttaaagaa
     3481 tggccaatat aataaaactg ctaatactaa ttgcaatgca tttgctaata tagaatgcca
     3541 atctccatta gttaaaaatc cttctaagaa tacccctaca tatggagggt caaatactgg
     3601 ctttgctaac catccccagt gcataaaaaa cattggtagt gtacctatta tagctccacc
     3661 aaatacatgt ggtataaaca ttattggatt taatattatc ggtgctccaa ataatattgg
     3721 ttcatttatc ccaaataatg cagggaataa tgatacacgt ccaatcttct tataggaagc
     3781 attttttgat aacatacatg caattacaca acctatacta attccacatc ctgtaaatcc
     3841 aaagaatgca cttgaagctc ctgctgtaaa gaaatgaggt attgtttctc cagcttgcat
     3901 agctgctatg ttttcagcta taaatgtaat cattataggt ctagttatag gactaaatac
     3961 tgatgaatgt attccaaaaa agaatatagt acatatagca aagttaagtg caagaactga
     4021 ccatgggtta tccatacttc ctactaatgg agataaaaat tgagtaagta tctgtggagg
     4081 taattctcca aatactttta atgaaataaa acgagcaact ataaaagttc cacaaactat
     4141 tatagtagta ggtattaatt caaaagacct agatacaaaa tcaggtacat tttctggcat
     4201 ttttattgtg atattcttct tattacaaaa attatataac tccacagaac caagccctgc
     4261 tatcatacct gtaaatagcc ctttagctcc taaatttcct atttctaaag ctccatctgg
     4321 tgtaaatgat gctactagga ataaaaaaga aaaagttgat agtgcaacac aacctggtat
     4381 atataattta tagtagttcc ccagattaaa agcaatacac atacaagcat ataatcctat
     4441 taaacctatg gaaagactta ctggtaaatc caatattgct gaattgttaa gtatgaattt
     4501 gcttattgga ttattttctc caagcatatt tggaagtgct ggaagtattg caaatatact
     4561 tcctaatatt gtgaatggtg tcatagcaac cataccagcc tttatagaac caaggtgacg
     4621 ttgattgtca accttatgag ctaaaggcat aaatactcta ttcataaatg cttcaaattt
     4681 tgaaaacata tctaatctcc cttttccctc attattttta ttatccttga gctaattgat
     4741 gtttcttata agttattaat gccaacttca taactgtagc tccatccatt tttccatata
     4801 catctttatc tattactgca aaaggcttat tttttggctc taatattcct tttaagaagt
     4861 ctattttgtg agctatttga ggacctataa gtactatatc ccaatcatca acttcacttt
     4921 ctattgaatc tgctggtata gctgtaaact caaaatcttt ttcattgagt ttagcactct
     4981 cttttaccac ctttttcata ttttgcatta acatattagt agaaaatcca cctgcacaac
     5041 ataataatat tttcataata attcctccta aagtttgtat ttttattatc tcttaaaagc
     5101 atctaaactt ttaaaagaat ttttacataa ataatctatt tctatattac aattacttat
     5161 caagacaata tatcaaagcc tctatcaagt cttttgctag ctgagaagtc atatagtggt
     5221 cttgagcatg taccattaaa agactcattg ctggactttc aacatcttca tttaaatctt
     5281 gagttattaa ttctgtttgt atcctgtgag cttcaatatc tattttacga gcctcctcta
     5341 ttaacaattt tgctccttta aaatcttttg acttcacttt ttttaaagcc tcaaaagctt
     5401 tcgatttact ctctcctgca gttgatataa gttccattat ttgtacttct gttgacattt
     5461 tcttattctc ctttagataa aatttcttat atgtttatat tgttcttcat caaaatttaa
     5521 aattaactgt gtatacaatc ttataatgtt ctctaaatct ttattactta ctattgaaaa
     5581 tgcacaatga ccatatctaa ctggtacaca agttactaca caaggtttcc cttcatttac
     5641 gagatgtgct tctcctccat cagtcccacc tgattcaaac atatcatgtt gaagtggtat
     5701 attattttta tcagcaattt ccattacata atctatcatt tttctactag ggactagagt
     5761 cctatctcta tgcattagca ttacaccttt atcaatttgt ttctgatttg tatagtctct
     5821 cacaaactca tttttagcac atgctacatc tattacaaat actatatctg gatttattaa
     5881 ctgtgttgat gtctttgctc cccttattcc aacctcttca ctacttgtag ccgcaaagtg
     5941 aatattacaa tttagatttt ctttcctaag ttcttttaaa acttctccca tcacaaaaca
     6001 tcctatccta tcatcaaaag cctttcccac taatcggtct ggtaaagtaa attcctcaaa
     6061 gcttgttgta tatgttacca tatccccaac attaatccct aggttatata catcttcttc
     6121 tgtatatgct ccaatatctg catatatatt ttcagcctta ccattattat atgtagcatt
     6181 tatgacagca gggattttct tcccttcttt tgttgtaatt ctaacttttt gtacaaactg
     6241 tgcaagaggt tttactcctc ctattgttat taagtgaatc ataccaagtt tgtctatact
     6301 tctcaccata aatccaactt catccatatg agcacaaatc attacgtttg gtgcactctc
     6361 atcttttatt ttagaaaaga tgatactccc tagcccatca cagataattt tatcactata
     6421 agcttttagt tcttccaata aaacatgtct aacctcttct tcattagatg ctataccatc
     6481 tgcttctgac aacctcttaa aaaactctat attcatgtct aacctcccag atagattttt
     6541 aacttataca taaactcttc ataagattgt gacttattta gcatgtctac catatcctta
     6601 tttgccataa tcttacttaa atatttagat atttgcttat gaagttctaa atttcccttt
     6661 attaatgaaa tcatgaaaat aattttaggc accctatttt cataaataat atcagtttgt
     6721 atcaaagcta ctgatattat atttttgttt cctgtcatct cgattggatg aggtatagcc
     6781 accccatttg tataaatagt actcaaatag ctttctcttt cccatacatc tttgggatag
     6841 gttggagaac aagcgccctc gtcaactata cattttgaca tactatcaag gatatcctta
     6901 tacttagctt tatcttttat acacctaaag tgattcttat caaatagacc taatatatat
     6961 tgttttgggc ttgatatatt tcctatatgg tctgcaaatc taacactttc tttaatatct
     7021 aaataatcca attcgtctaa aatttcattt ataagtataa ctggagcatt tatttcaaat
     7081 aaaagatttg ttattgaaaa aatcaaatct ggtgcaaatt ctattacagc tttttcttct
     7141 agtaatgaaa aatttcttat ctcagcattt ggaaaaattt ctttaagtct taaatttatc
     7201 aaaaatgcac tacctccacc agaagagcat attattgcta ttttatattt tttattaaaa
     7261 ttagcttctt ctctcttttc aaaaggaaca gcaatgtgag tagctataaa ccctatttca
     7321 tcttgactta tttttatatg atactcattt tctatgattt ttgaaaattg aatagataaa
     7381 ttaaaaacag ttggatactg ttgacttatt ttaactgaaa aaggatttat tatcttgtgg
     7441 ttcttcttta tacgttctat taaacatgca atatgtaagt aaaataagtt aaagaactct
     7501 ttatcttcta aaaaattaga gttatatttt ttatctatct ctctaaaaaa ttcgaacata
     7561 atatcttgaa gtttattctt atcaatattc tttatagtgg ctttcttatc tttagtcttt
     7621 agttttataa gctcctttaa ataatacttt tcatcataat taaggttata ttttttatct
     7681 ctaaaaattt tatctaataa tttatctata attaaatctt ctgtatataa ttttaaatcg
     7741 ctagcatttc ccgctactct catactaact cttatataca atataataat ttgtgctaat
     7801 atttcctcaa gttctgttaa atttgcttct aaatcatgtt cattaagtac attttttatt
     7861 gtacttctaa tattagctaa ttttaaatta tctataaact tattcctgta ctctgttata
     7921 tttctattcc ctctaaaata actatccacc aatatacttt gaatttcttt tatagaacct
     7981 tctattttaa ttccataatg agcctttcgc tctagtttta attctgtatc ctttatcttt
     8041 tcatctatct tttttaaatc attttttatt tgggatgaac ttactagaaa ttttttactt
     8101 aactgttcta tagttttata accttcattt actaaaagtt ctactattat agactctaac
     8161 cgttgttctg gattttcaac taaatctgag ggtaagtttc ttagataatg ctcgaattta
     8221 tcttggtctt taatatttag aatatatcct aatttagact tatattctaa tttaaaacca
     8281 ttacattctt ttatagtttc tatctttttt atttcatttc tgatagttcg agtagatacc
     8341 cccaacttat ctgcaaaata atctatgttt agataaccac tactattttt taatgtttca
     8401 agaataatgc gttctctcat atccccacct ccctcagtgt tattattgtg tttttttaag
     8461 tggtataaaa aatatttttt ataaaaacaa tcttttcttt ttatttaaga attttatatc
     8521 ctagtatatt ttaattccac aatttaatat aaatatttta taataataaa atatatagtt
     8581 caatttttag tatattttaa ttatatatgt aatactttac caataaaagc tgatattttt
     8641 catcaattaa atgaaaaaaa tacaccattt acatataatt aatataaatt tttcatataa
     8701 caaatatcct aaacttatct aaaataaatt taggatattt tatttttata ctctatttac
     8761 agacccacac actttaatct tttctttcac aaaatccttt actgattgaa gccctgctaa
     8821 tccatacttt ttagggtcta taacctcaga atcttcatct aaaacttttt taaccccttt
     8881 actatatgca attcttaatt cagttgcata gttaactttt gcaatacctc tttttatagc
     8941 ttcttttata atatcatcat gtattcctga acctccatgc aatactaaag gtattgaaac
     9001 tacattttta atttctgata acctatctaa atctatttta ggttcaccct tatacaatcc
     9061 atgtgcagta cctatagcaa tagccaatga agatattcct gttctatcga caaactcttt
     9121 tgcctctttt ggatttgtat aatcattact attcccgcca tctaaatcat cttcttttcc
     9181 accaactttc ccaagttctg cttccactgg tataagagaa ggactacatg cttttactac
     9241 tgatttagat atagatatat tattttcaaa gctttcatga gaaccatcta tcattattga
     9301 tgtatatcca cttcttaaag ctttcattgc aaggtcaaaa cttgaaccat ggtccagatg
     9361 cattgctaca ggtatcttta ctctttctgc agcaaccttt gcatttgcta gataataatc
     9421 aagacctgca tattttacag tagatggagt agtttgtaat attactggag aatttaactc
     9481 ctcagcagct tctataacag caattaccat ttccatattt tctacattaa atgctcctac
     9541 tgcataccct tcatcttgtg cctttttaag cattgatgtt gttgttacta atgccacatt
     9601 gataccctcc caaattattt agttataata ttttaatctt atcttttaat attcatatat
     9661 tgttacacct tttactactc gatttactgt tcctgtagct gatggcgtat ctggcttatt
     9721 tccaaccttt accgatacaa acaatgctat agtttgtgca aacaatatgt atggcattgc
     9781 aagatacaca tctggtaatt tattatactt ttctttaaac tcaaactttg ttcctgagaa
     9841 gttattttca ttttctactg acactgcaca agtgaattta gcaattttat ccctttttat
     9901 ctcctctaaa acatctaaat catattttct tgtatataaa cagttagata caaattcaaa
     9961 tacaagtgta ttttcatcaa taaatgattt tggtccgtgt ctaaacccca taggagaatc
    10021 atacactgta gatatttttc ctgctgtaag ttctaaaagt tttagttgtg cttcttgtgt
    10081 caaaccacct aatcctcctg aacctaaata tacaactcta tcaaaatctt tatttattaa
    10141 ctcatgtatt tcatcttttc tatctataac atttcttccc atttcaacta ttgcatttat
    10201 atatgacttt tctacatcat cttctaaact atcgaatatt aacatagcag ataacatcat
    10261 acacgaaaaa cttcctgtca tggcaaagcc ttcatcattt gattttgatg gcattaataa
    10321 caagtaatta ttttcgtcgt ttttagtcat cttagctagt tctccttctg gattacaagt
    10381 tatagctaaa tgataaaaat tatctacaat cttattccct aaatttaaag cagccaaact
    10441 ttcaggacta tttccacttc ttgcaaatga aattaataat gttggtatat ctttttttaa
    10501 gtaatcatat gggtttgaaa ctatatccgt agtaggtatt gcttcaaata tgtattttct
    10561 tatgtcattt ttatttttaa ggtaaggaag tattgaattt cctatgtaag ctgaagttcc
    10621 agcacctgta aatataactc taaactgccc atgattatta cttattgtat ctatgaatct
    10681 acttaatttt tctttgttag atttatatat ttcataagtc tctaaccata attcaggctg
    10741 ttgttttatt tcattagttg ttattattgc acctaaatct tttagttttt gttcttctaa
    10801 tttaaacatt ttcattctcc tttcatactt gtaacgttta catttggtaa tgaggttata
    10861 accactttaa actataaaaa aatatactct tttataattt tttgcttaga tttttagtac
    10921 tacttaaact cactataaaa tatacactat agattttaat taattttata gtttatcttt
    10981 aaaaatttaa taaaatcata ctctatatac tttagtattt ttattttacg ataaaaactt
    11041 gttcagacca aagtggtaat taccactaat ccaaatataa attaatttga attttttgtc
    11101 aatacataaa atatttatat caatcaattt tatagatatc ctacaaaatt actttatcaa
    11161 tatttcttat ttcgctaatt tacttatata agctttaaat ttattttttg atacaaagtt
    11221 tacaaattta tgtgtctgaa ttataatcaa ttctgtaaat ataaaaacct taggagaaag
    11281 tattatgaaa aatataaaaa tgggaatgat aggtcttggc tccatagctc aaaaggcata
    11341 tttacctata ttaactaagt ctgagagatt tgagtttgta ggagctttta ctccaaataa
    11401 agttaaaagg gaaaaagtct gtagtgatta tagaatcatg caattcgatt ctattgaatc
    11461 cctagctaaa aaatgtgatt gtatttttct acatagctct acagaaacac actacgaaat
    11521 tataaaaata ttattaaatt taggtgttca tgtatatgta gacaaacctc ttgcttcaac
    11581 agtaagtcaa ggagaagaac ttatcgaatt aagtacgaaa aaaaacttaa atttaatggt
    11641 tggattcaac cgtagattct gtcctatgta caaagaaatt aaaaataatg ctacagaaat
    11701 tgtatctatc aatatatgta aacatggatt aaattcctta agaaatgtta aattcgacag
    11761 tactcttata gatgactata tacatgttat tgacactgct ttatggttag caaatgaaga
    11821 tgttgaaatt agtggagagg atttattttt aacagacaat aagaatctta tatttgtaag
    11881 tcataaactt aaaggtaaaa atttctctat aaacacctct atgcatagag attctggaac
    11941 aaagttagaa caagttgaaa ttctttcaaa gggtaagatt caaagagtta agaatcttaa
    12001 tgtactggaa atagaagaag gtggaaacct aactttaaag caaagtggtg catgggtaaa
    12061 tatattaaaa caaaagggtt ttgaggatat atcaaatcat tttattgatt gtatagaaaa
    12121 taatataaaa ccagctatca gtggagaaga gtgtatcaag gctcaaagat tactagagaa
    12181 aattataaat tcagtaaaat agatatttat tataaaaata ggttaaaaat gaatttttct
    12241 aacctatttt tatataagac tatatctttt atattaaaat taaaatctat tacaacaaca
    12301 ttaaatttat tatattccca ttaaatgtat ttaacttaac ttttttactt caactaaatc
    12361 ttgatattta tcaatttctc ccaaatctac aaatccagtt tccatattta aagcattact
    12421 tataccacaa gcatttgcaa atttaagtaa attttctatt ttataacccc ttaaaatacc
    12481 tacactaaat ccagccactg tgctatctcc actacctaca gtatttatac actttacaac
    12541 tggtactttt acataatata cgttatcttc gtttaaatac accataccat cttttcctaa
    12601 agatatacat acattctctg cacccatttc aataagtttt tttcctgaca agataatttc
    12661 ctctcttgat tctatattta ttcctaataa atgtcttatt tcatctatat ttggctttat
    12721 taaatatggt tttgatttta aagctatttt taatgcttct cctgatgtat ctaatataaa
    12781 ctttatatta tttgctctac atatatttcc tatcttttca taatagtcta tcggcatatt
    12841 ctcacaataa ctaccagaag ctaccagtat tttagtattt tttagtactt cctctatatc
    12901 attttcaaat ctctctaaat cgcttttttc tactattggt cctttttcta aaaattctgt
    12961 actaacttta ttgtcctcaa ttatgtttaa gcaatttcta gtttcctgct ctatttttgt
    13021 aaatttattt tgtatatcta gttttctaag ttcatttttt ataaattctc cattaaatcc
    13081 gcctagaaat cccatagcta aaggattttc ttttaaaatt ttacatacct tagatacgtt
    13141 taatccttta cctccagcag ttgcattctt acttactacc ctttgcacct cacctatgtt
    13201 tatattattc actctataca ttctatctat agatgggttg aatgttatta ctgtaatcat
    13261 actagattct cctcattatt aatttttact tttctaaaca ataaattaat ctgtaaaata
    13321 aaaaatacct catataatat atccaaagtg gttattacca atttgaatat aaattatttt
    13381 acttgttttg tcaatgtacc ataattttat aataaattaa tagccttact tattgaattt
    13441 tgattaaact ttattcattg acataaataa ttctatattc tataatcaaa ttgtggtaat
    13501 aaccacttag aaaggactat atttttatga taaaaagaaa tgataaaaga ccaatatatg
    13561 accagcttgt tgagatatta agaactaaga ttgaaaatga gatggaacca aacgatagaa
    13621 tgctttccga aagaaaaatt tgtgatgaat atggagtaag tagaaccaca gttagactag
    13681 ctatggctga gttagagcat atgggatata tatacaaaag acatggaaaa ggaacttttg
    13741 ttgctgcact aagtcaaaat tctcaaaact taatggaaag ttatagtttt acagattata
    13801 tgagaacaca aggaaaaaag ccaggtacaa aggtattatc atttgaggta gtagaaagta
    13861 caaagtattt ctctgaaaaa ttaggtataa ctcctggtga aaaaatgata aaaataatgc
    13921 gtttaagact tgctgatgac cttcctatga tgcttgaaag aacatatctt cctatgaaag
    13981 aatttatagg attgacaaaa gaaaaaatcg aacaaaaacc tttatatgaa atatttagag
    14041 aagattatgg cgagattatt aaagttgctg atgaagaatt ttcagctgga ataatctcgg
    14101 ataaggaagc taagttattg gaagtaccag gagattcacc ttgtctgaaa ttacttcgta
    14161 ccacatataa tgatgacaat atagttattg aatttacttt gagtgtcgct agaagtgata
    14221 aatttgtata tagagtaaga catgtaagat agtaagttaa ataactttca agaaaacaga
    14281 atgatattat aagcgtcaat gccacaatga taatataatt ataaggaggt tattttgaaa
    14341 agttttattt atgcagataa attttttatg aaacatggaa tcaaagaaaa aggttacctt
    14401 agtataatta atggcaaatt cggtgaattt caacaagaaa gacctaatgt tactgataat
    14461 gttatagatt tttcaggtaa atatattgct cctggcttag ttgatactca cattcacggt
    14521 cttttaggtg cagatgtcat ggacaataca tttgaagcaa taaacacaat ttcaaaaggc
    14581 ttattaaaat atggagtaac atctttccta cctacaactt taactgattc tgtagataca
    14641 ctaagtgatt ctgtagaaaa tataagcaat agcttcaatc gagttgaggg tgcaaaaata
    14701 caagggattt atttagaagg tccatttttt acagaaaaat ataagggcgc tcaaaatgaa
    14761 aaatatttta aagaccctga catcaatata ttaaaattat ggcaaaaaaa atcgaaaaat
    14821 ttaataagaa aaatagcaat tgctccagaa cgtaaaggtt caatagaatt aacaagatat
    14881 gcaactaaaa ataatatatc agtttctctt ggtcatagtt cagctacatt tgagcaagtt
    14941 aaacaagtcg ttaattcagg tgctaaagtg tttgtacata catataatgg gatgagtcct
    15001 ttaaatcata gagaacctgg aatggttggg gctgctatgg tactaaaaga tacttatgct
    15061 gaattaattt gtgatggtca tcatgtaagc ccaatagctg ttaaaataat gatggatgct
    15121 aagtctagta ataacatagc tttaattaca gattgtatga gagctggtgc tatggaggat
    15181 ggaaaatata ctcttggtaa gtttaatgta aatgtaaaaa atagtgttgc aagattaaat
    15241 agtgggagtt tagctggaag tgtattaact atggacaagg ctgtgagaaa tatagttgat
    15301 tggaacatat caactttaga agatgcaata aaaatggcta catatgtccc tgctttaagt
    15361 tgtaatattg ataatgtctg tggctctata tatcaaggta tggatgcaga ctttatagta
    15421 acagataatg actttaatat acacgaaact tatataaatg gtatctgtaa gtacaaagtt
    15481 aataaatagg ttcaatataa ggttatggag gctgtctaaa aagagtctgg aaatatttaa
    15541 agttatttct ccaaaatctg atttaacact tttatttcca tatcgttaat aaactattgc
    15601 gatagaggtt attttaaaca aatatatatt ataagtattt ttgtgataat attttcgata
    15661 atatgtacat ttaaagtaac ttttctatta ttatttgtaa gtttttatct tgaacaaaca
    15721 tataaatttt taataaagca tgagataaac cctatatttc aattactaat gatgagattt
    15781 tgattgataa cagatatatt ttcaaatgta aaaatactaa aaatcatatc aattttaaag
    15841 atattgtagg tgtaaataaa caagataata ctatagaaat ttttacaaat gaaagcactt
    15901 attgtatata tctaaaagtt ttaagcattg agagcgaaga atatctaata gatagactaa
    15961 aatcattttg ttgattttat atattttatg ttaaatataa agcagagtag ttaaaactac
    16021 cctgcttttg taattaaaat ttaaaaataa taactcataa tacattgaat ttgagcttaa
    16081 cagtaactat tgtaataata gcatcaatct tttgtgttga atataattta tttacactat
    16141 agttaagcat gatttctaat gttattttct ctaatttaca attatgatta aaattcatta
    16201 atatatatat atttataagt tgtattgttt atattgttta ttactttttt aaatccacta
    16261 gctgtaataa taaatgtctt ttctggtatc cctggtgaaa agattatttt atcatggtta
    16321 aatatatctt gatctattac aagagagata tttttctcat atccaaaagg agatacacat
    16381 ccaggcaaac attgtgtttt ttgtgttaat tcttcttttg aacatataga tacttttcca
    16441 cctaaaacat tttttatagt ttttggatct aatttttttc cttctgtggt tacaaatata
    16501 tagtaatttc cagacctatc ttttataaat aaacttttac tttctactcc agtatattgt
    16561 aatttttctc ttatttcttt agctttttca aatgataaaa taggttcatg gtcatattct
    16621 tcaaacgtta tatgattttc ttttaaaata tttttgatat tgttgtacat atattttcct
    16681 ttctatattt aaattaattt atatataata ttcaattata aagtgatttt ataatttctt
    16741 caaactatca taaaaaacat tcacatgttt taggaa
//
LOCUS       DAEMGG010000088        16688 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEMGG010000088 DAEMGG010000000
VERSION     DAEMGG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.088"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1157)
                     /gene="ribB"
                     /locus_tag="KPK24_002601"
     CDS             complement(<1..1157)
                     /gene="ribB"
                     /locus_tag="KPK24_002601"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBG2300267.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKT"
     gene            complement(1180..1845)
                     /locus_tag="KPK24_002602"
     CDS             complement(1180..1845)
                     /locus_tag="KPK24_002602"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG2300268.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(1879..2991)
                     /gene="ribD"
                     /locus_tag="KPK24_002603"
     CDS             complement(1879..2991)
                     /gene="ribD"
                     /locus_tag="KPK24_002603"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG2300269.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3123..3244)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3433..5916
                     /gene="recQ"
                     /locus_tag="KPK24_002604"
     CDS             3433..5916
                     /gene="recQ"
                     /locus_tag="KPK24_002604"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG2300270.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6005..6116
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6258..7559
                     /gene="thiC"
                     /locus_tag="KPK24_002605"
     CDS             6258..7559
                     /gene="thiC"
                     /locus_tag="KPK24_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG2300271.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7613..7807
                     /gene="thiS"
                     /locus_tag="KPK24_002606"
     CDS             7613..7807
                     /gene="thiS"
                     /locus_tag="KPK24_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG2300272.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7800..8603
                     /gene="thiF"
                     /locus_tag="KPK24_002607"
     CDS             7800..8603
                     /gene="thiF"
                     /locus_tag="KPK24_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG2300273.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8666..9436
                     /locus_tag="KPK24_002608"
     CDS             8666..9436
                     /locus_tag="KPK24_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG2300274.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9453..10556
                     /gene="thiH"
                     /locus_tag="KPK24_002609"
     CDS             9453..10556
                     /gene="thiH"
                     /locus_tag="KPK24_002609"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG2300275.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10557..11246
                     /locus_tag="KPK24_002610"
     CDS             10557..11246
                     /locus_tag="KPK24_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG2300276.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            11434..12828
                     /gene="yfcC"
                     /locus_tag="KPK24_002611"
     CDS             11434..12828
                     /gene="yfcC"
                     /locus_tag="KPK24_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG2300277.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            13345..14712
                     /locus_tag="KPK24_002612"
     CDS             13345..14712
                     /locus_tag="KPK24_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG2300278.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            14737..15345
                     /locus_tag="KPK24_002613"
     CDS             14737..15345
                     /locus_tag="KPK24_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG2300279.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            15393..15764
                     /locus_tag="KPK24_002614"
     CDS             15393..15764
                     /locus_tag="KPK24_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG2300280.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            15943..16281
                     /locus_tag="KPK24_002615"
     CDS             15943..16281
                     /locus_tag="KPK24_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG2300281.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
BASE COUNT         6461 a         1821 c         2838 g         5568 t
ORIGIN      
        1 ttagttttta aatatagctt attaaagtca ttaatttctt cattaatagg gattctattt
       61 tcgattttta ttccatattt ctctaattga tttattttat ctgggttatt acttaaaaga
      121 tttatacttt ttatgtttaa attttttaat atttgagcag ccatatagaa atctctcata
      181 tcttcttcaa atccaagcat taaatttgct tcaacagtat cgtaaccttc ttcttgaagt
      241 ttataagcct taattttatt taaaagacca atacctctac cttcttgctt catataaatt
      301 aaaactccac ttccattttc cacaattttc ttcatagaag attctagctg taatccacaa
      361 tcacatttta gagagtggaa cacatctcct gttaaacatt cagaatgaag tctaactaga
      421 gtattttcta tatttatatt tccataagta agggcaatgt gttcttcatt tgaataagta
      481 tctctatatc ctatgatttc aaaatttcca tattttgtcg gaaggaaagc actagatact
      541 ttttctatag tagtttcatt tattttgcgg tattctataa ggtcttttat agtgatgatt
      601 tttaaattat gcttttcttt gaaaatcatt aaatcatcaa ctcttgccat tgtaccatca
      661 tctttcatta tttcacagat aagaccaatc tctttaaaac ctgcaagtct agcaaggtct
      721 actgaggctt ctgtatgtcc atccctgaca agaactccac cagtttttgc tattaaagga
      781 aagatgtgac caggcgtttt gaaatcttta cttgtagaat tttcatcaca gaataatttt
      841 acagtttcag ctctttcata tgcagatata ccagttagag tatctgctcc atctatagta
      901 acagtaaagg ctgtttgaaa tgtatctgta ttttctctaa ccataagagg tatattaaga
      961 cttttaaatt ttttttcagt ggcaggtaga caaattagac ctctaccata tgtagccata
     1021 aaatttattg attcaggagt agccatttca gctgccatca ataaatctcc ttcattctct
     1081 ctactttcat catcaactac gattaccatt ttcccatttt ttatatcttc aatagcttct
     1141 tcaatagtat taaacatgta attcactcct ttattatttt taaaaaccat ttttaaataa
     1201 aaaatcttta tctatatttg atttatggtt actactatta gaatttaatt ccttgtagtt
     1261 attagccata aaattattaa catactttcc aattacatcg ctttctaaat taacgaaatc
     1321 tccaacattc tttgtcaaga gaatagtgtt aattttagta tgaggtataa tagaaacctt
     1381 aaatatttca tcatctaaat atgatatagt taaacttaca ccatcaatag caacagaacc
     1441 tttgagtatc attgaggata aaagtttttt atcaggtcgt atacttacta aaactgcatt
     1501 ttcatctttt tcaaatttgc agatagttcc tttaccatca acatgacctg ttactatatg
     1561 accaccaaat cttgaagaaa gactcatagc tctttcaaga tttaccttgt catttgcatt
     1621 taataaacct aaatttgtgg aacgaattgt ctccatcata acatcggctg taaattcatt
     1681 ttttccgagg tttgaaacag ttagacaaac tccatttacg gctatactat ctccaagatt
     1741 tgtgccatct aatattttat ttgatagtat ggttactttt ccactcttac cattagcagt
     1801 aatttttcta agtattccaa cttcttctac tatgccagta aacatcctaa cctcctatat
     1861 gaatgtatta acaaaaattt aatttaaaac atctgattct ataagaatat cgccatctat
     1921 caaagttaca ttattgattg ttaactgtgt tgaatctgac aaatgattta ttccttctcc
     1981 agaaacgaat gtaggtgcat ctttaccacc tataatcttg ggtgatagaa ataatttgac
     2041 tttatcaact aacttatttc taaagagact gtcattgaga gtagcaccac cttcaactag
     2101 aacagaatca atgtttaact ctcctaattt atacattaca aactccaaat ctactaagtt
     2161 gttttttgaa ggagaaatta ttgtttcaac attattttct tttaacatag ataattttat
     2221 actttctttg caacatgtga aaactatagt tttactggtt ttatctttaa ccaaattact
     2281 attaagtggc aactttaggt gtgtatctat tactattctt gtaggatgag acactttttc
     2341 ttgattgagc ctacaagtta gtaaaggatt atcgttcaaa actgtattta tgccaaccat
     2401 tatactttgg tatttatttc tgtatttatg agttagatac cttgatgatt cattagatat
     2461 ccattttgat tctagtgatt ttgtggcaat tttaccatct agacttactg ctgattttac
     2521 aatacaaaga ggtcttttgt tttttatatg ataaaaaaat gcctcattta aatctcttgc
     2581 ttcatcttct aatattccca cagtaacatc aatattgtta tcttttaatt tttttactcc
     2641 attaccacaa acaagtggat ttgggtctaa tgtagatatt acaactctct taatcttatt
     2701 ttgtataatt ttatctacac aaggaggcgt tttcccataa tgagaacatg gttctaagtt
     2761 tacataaaga gtagagtctt ttagactttg tttagcactg tttatagcat ttacttcagc
     2821 atgattagag ccaaatttct catgatatcc tttgcctatt atattactat ctttaacaat
     2881 tacacatcca actaatggat ttggatttac aaagccttct ccatttttag ccaattcaat
     2941 agcttttttc atataataaa tatctttttc tttttgattc acaaaattca caatatgtac
     3001 acctcctgtt taattggata atttttaaac aaaaaaccct gagaattaac tcagggttta
     3061 gaataatcta caataaatgt ttaataaatg tagaacaaaa ttgctctaca aaaacactca
     3121 ttaaatatct tcttccatcc agactttact gtcggctttg gaatttaacc aaatcaacca
     3181 agttttacat tggctcgcgg gctataccgc cgattgggaa tttcacccta ccccgaagat
     3241 taagtattaa atttatattt agaatgtatc ataaattatt ttactttgca aactattttt
     3301 ttaaaaaata tatgttaata tataatatag aatgtctata aactttgaaa taccacgatt
     3361 gtgaatataa aaataaaaat ttgaacataa tcaggaaaga agtctaatta atttaaatta
     3421 ggagagtaat atatgaataa aaaactttta gaaatactat caaaatacta tggatataca
     3481 agttttagaa aaggtcagga atctataata aattctatct taagcgaaaa agatgtgcta
     3541 gctattatgc caacaggagg gggaaaatca atatgttatc aaattccagc tttgatttta
     3601 gatggaatga caatagtaat ttctccactt atatcactta tgaaagacca agtagatgcc
     3661 ttaaagacta tgggaattag tgctgctttt ataaatagtt cattaagtag caaagaattt
     3721 aatgaaatat taaataatat tagaaaaaat aattataaaa tactctatat tgctccagag
     3781 agattagatg cacaagagtt tattgagcta ataaataata acaatgtaag tcaagttgcg
     3841 atagatgaag cacattgtgt atctcaatgg gggcatgatt ttaggttgag ttatagaagg
     3901 atttctgatt ttataaaaaa tttgcctaaa agacctatag ttacagcatt tactgcaaca
     3961 gcttcagaag aggtaagaac tgatataata aatttactgt gtttagaaaa tccagattat
     4021 tatataactg gatttgatag agagaattta tcaataaata tagtaaaatc atctagtaaa
     4081 aataagtata ttttagatta tattcaaaat cataaaaatg agtctggaat tatatatgtt
     4141 gcaactagaa aggaagttga aaatatatat aatggtttat ctaagagaaa tatatctgta
     4201 tcaaaatatc atgcaggttt atctaaaaat gaaagaagta aaaatcaaga agattttata
     4261 aatgataatg tggatataat ggtagcaaca aatgcatttg gaatgggtat tgataaacct
     4321 aatataagat gggttataca ttacaatatg cctcaaagta tagaaaacta ctatcaagaa
     4381 ataggtagag ctggtaggga tggagaaaaa agtgagtgtg tacttttatt ttctccagga
     4441 gatgtacata tacaaaaata tttaattgat attggagttg aaaatcctga aagaaaatta
     4501 tttcagcata agaagcttca atacatgatt gacttagttt acagtaattc atgctataga
     4561 aagacaatat tgagttattt tggagaaaat tatatagaca attgcaataa ttgtagcaat
     4621 tgtttagtag agggtgaaat agtagataaa actatagatg cacaaaaagt catttcatgt
     4681 gtagctagaa tgaaaagaag ttatggtgtt actactattg tagatgtgct tagaggttct
     4741 aagaataaaa agattttaca gcttggatta aatactttgt ctacatataa cattatgaga
     4801 gattattcta gtgaagactt aaagaatttt gtaaatacat tggtttctca tggtttttta
     4861 gatttagttg agactctagg caatggaaat agaggtagtt ttcctactat aagattaaat
     4921 gagatgtcta tgaaagtttt aaaaggtgaa gtgaaagtag agtttaaaga aattgttatg
     4981 acaaaatcat tggaagtaga ggatgagtta tataatgctt taagagaact tagacattca
     5041 attgcaagtg aagaaaggat agctccatat atggtatttg gagatggtac acttagagcc
     5101 atgtctagta gttatccaat taataaagaa gaaatgctag atatctctgg agtaggagaa
     5161 attaaatacc aaaaatatgg tagagatttt gaagcagtaa ttaaagaata tgtcgaaaaa
     5221 aataatataa ataaaaaaat agatgaaaaa aatgtcgatt caaagaacgt taatagtgat
     5281 ttcttagagg ttaatacaga taaagtttta tatgaaaaac tgttagtcat tagagaagaa
     5341 tatgcaacaa aggaaaaatt acctgcatat atgattttag ccaataaggc tttgaaagaa
     5401 ataagtggaa gatatccact tgatgaagaa caattaaaag atatttctgg cataggtgga
     5461 gtaaaatttg aaaaatatgg agctagtatt ttggcagagg ttaataaata tgtaaaagaa
     5521 aataatatta atgttatttg ggagaaaaag ggcaaaagga agttaatatt agatggagaa
     5581 agtagaaaaa ataatgaaat agcattagat ttgctaaatc aaggaaaaga tatacaaagt
     5641 atcagtcaag aaatagaagt ttcactgtca accattattg gatatgtgca tgactatgta
     5701 aaggatggac atagtatatc atttgaatta ggactagaaa atttttataa tgaagataaa
     5761 aagaagatga ttttagaagt ttgcgaaaaa gttggctatg ataatttaaa taacataaaa
     5821 agaaaacttc cagattcaat aaagtatgaa aatataagag ctgtgatact tgactcttat
     5881 cttgaaaact taaaaaataa tattatagca gagtaatttt taacttaata aaaaataaaa
     5941 tttgtattat tgtgttgaac taataatttt gctgtgttat actgaatttg taaaaataat
     6001 gaaaaattag agctaggggc gctagaattg gctagctgag agataaagcc gtaacttaca
     6061 aaccctaata acctgatcaa gataatgctt gcgaagggaa gctttttaat ataattttat
     6121 tatataaata ggcaaattat tcaatgttag gagaaatttg tctaagtgta tatacgatta
     6181 taagagtgct ttccaaacga ggaaaagcac tttttttatt ttcaaaaact ataactttaa
     6241 tcttggagga ttgtaatatg aactatacaa cacaaatgga tgctgctaga aaaggcataa
     6301 taactaaaga aatggaaatc gtttctcaaa aagaacaggt agatgtaaat gaattaagag
     6361 aattaatagc aaatggacaa gttgttatac cagctaataa aaatcataaa tctttaagtg
     6421 cagaaggtgt aggtaaaaat ttaagaacaa aaataaatgt aaatctagga atttcaagag
     6481 attgtaagga tatagaaaaa gaacttgaaa aagttagagt agcaatagac atgaaagctg
     6541 aagctataat ggatttaagt aattatggca aaactagaga atttagagaa aaggttgttg
     6601 aaatgtcacc tgcaatgata ggttcagtac ctatgtatga tgcagtagga tatttggaaa
     6661 aagaattaaa agatataaca gaagaagagt ttttaaatgt aataagacaa catgctattg
     6721 atggagtaga ctttattact atacatgcag gtcttacaag aagtgtttgc caaaaaataa
     6781 aaaatcatga gagattgact catatagtat ctcgtggagg ttctttattg tttgcatgga
     6841 tggaattaaa taataaggaa aatcctattt atactaattt tgataaaata ttggatatat
     6901 gtgaagaata tgatgttaca ttgagtttag gagatgcttg tagaccaggt tgtataaaag
     6961 attctactga tggtgttcaa attcaagaac ttgtagtatt aggtgaactt actaaaagag
     7021 catgggaaag aaatgtacaa gtaatgatag aaggtcctgg acacatggct attgatgaaa
     7081 tagaagcaaa tgtagtatta gaaaagagat tatgccatgg agcacctttt tatgttcttg
     7141 gaccattggt tacagatata gcaccaggtt atgaccatat aacaagtgca ataggaggag
     7201 ctttagcatg tgctaaagga gtagattttt tatgttatgt aactccagca gaacatttaa
     7261 gattgcctaa tttagatgat atgaaagagg gtataatagc tgcaaagata gctgctcatg
     7321 caggagatat agctaaaaat gttaaaggtg ctcgtgaatg ggataataag atgagtaaag
     7381 ctagagctga tttagactgg tgtgagatgt ttagacttgc aatagaccct gaaaaagcta
     7441 agagatacag agatgaatca actcctactc atgaggatag ttgtactatg tgtggaaaaa
     7501 tgtgctcaat gagaacagtt aagaaaatat taaacaatga ggaacttaat ctaatataat
     7561 ttttggctag tatgactgga ataaaaataa gctgaaaaga ggaggtgatt tagtgaaggt
     7621 taatgggaag gaaattgaat ttgaaaaaga tttgactgtt atagatttat taaataaata
     7681 taatttaaaa agtgatagag ttgtagttga agtgaattta gagataattg aagaatctaa
     7741 ctacaataca tatgtattaa aagacgagga cattgtagag cttattagct ttattggagg
     7801 tggttaaaat aaaaatattt gtaaatgaat tattaacaga tgttgaggaa aatacaactg
     7861 cttataaagt tagagatagc tataacaata aatgtgatgt attggtgtta aatggatatc
     7921 caattaaaac agatatgacc ctttgtgaaa atgataaatt aacacttata caaaaaggag
     7981 ttaagccatc acttgatgaa ttggaaagct tgttgattgc tagacatact ccaaatatac
     8041 ataataaatt aaaaaaaggg aaagttgcta tattgggatt ggggggactt ggttccaata
     8101 tagctatctc tttagctaga ataggagtgg gagaactttt acttatagat tatgatgttg
     8161 tagaaccatc taatttaaat agacagcagt attttataga tgatatagga aaactaaaaa
     8221 ctgatgccat gatagaaaat ataaaaaaaa taaatccatt tataaaatta aataaacgag
     8281 atattttttt aaacaagaac aatatggata ctattaagga ttcagattta ataattgagg
     8341 cctttgatga tgcttcatgt aaagcacagg tatgtaatta tgtattaata aatttaaaag
     8401 ataaatattt aatagcatca tctggtatgg caggttacta tgattcaaat ataataacta
     8461 ctaagaagat aaaagacagg ttttatatat gtggggattt tgtgaatgaa gctaaatttg
     8521 gtgaagggct tatggctcca agggttgcca tctgcgcaaa tcatatggca aatttggcaa
     8581 cgcaaatact tatagatatg taaatattaa aattagatat aaaaaagatt gaatattaaa
     8641 aaaatttaac gtggagggaa ataatatgga taaattggta cttggtggac atgaatttaa
     8701 tagcagatta ttggttggaa caggtaaata tggttcaaat aatatattgc ctgaggtaat
     8761 aaaagaaagt ggaagtgaaa taataacaat ggcacttaga agagtggatt tagacaataa
     8821 acaagaaaac atattaacgt atattccaaa agagatgaca atacttccaa atacatcagg
     8881 agctactaat gcagaagaag ctgtaagaat agctagaata tctagaaaaa tgggatgtgg
     8941 agattttata aaaatagaag taatatcaga tactagatat ttacttcctg ataatgaaga
     9001 aactataaaa gctacaaaga tacttgctga tgaggggttt atagttcttc catatatgac
     9061 accagacctt tatgctggaa gaagacttat agaagctaat gcagcggcag ttatgccact
     9121 tggagctcca attggttcaa atagaggtct tcaaatgaaa gaaatgataa gaataatgat
     9181 agatgagttg gatataccaa taatagtaga tgcaggtatt ggaaaaccat ctcaagctat
     9241 ggaggctatg gaaatgggag ctgatgcagt acttgtcaac actgctatag cttcagcagg
     9301 agaccctgta caaatggcta gagcatttaa attagctgta gaaggtggaa gagaagcata
     9361 tattgcaaaa acaggaaatg tgtcagaatt tgcaaatgca tcttctcctt taacaggttt
     9421 tttaggcaat ttatagaaag tggtgattgg tgatgagttt ttatgatgta atagaaaaat
     9481 atagggattt tgattttgat ggatatttaa ataatgttac tgataatgat gttttaagaa
     9541 gtttatcaaa agacaagctt gaagattttg atatattaaa tttgctttct aaaactgcag
     9601 taaaacattt agaagatatg gcacaaaaag cacataaatt aagtgttcaa tactttggta
     9661 aaacagtatg tttatataca ccaatgtata tagcaaatta ttgtgttaat caatgtgttt
     9721 attgtagcta taatataaag agtggaataa aaagaaaaaa attaactatg gatgaaatta
     9781 gagaagaggg agaagcaatt tcaaaagaag gtttcaaaca tctacttgtt ttgactggag
     9841 aaagtagttt tcactcaagt gtagaatata taggtgaggc tatagaaata ttaagagaga
     9901 aatttccctc tataggtata gaagtttatc caatggaagt agaagaatat aagtacattg
     9961 tagataaagg tgttgaagga ttaacagtat atcaagaaac ttatgacgag gaaatatata
    10021 agagagtaca tataaaaggc ccaaaatcaa attataaatt tagactagat gctcctgaaa
    10081 gaggggcaaa agcaggtatg aggactttat caataggtgc acttttaggg ctaaatgatt
    10141 ttagaaaaga aacttttttt acaatattgc atgggaaata cttaaaaacg aaatatcctc
    10201 atattgagct ttcatattca acaccaagaa tgagaccttt taaagggtgt tttgaggaat
    10261 tggtagatat aagtgatact gatttagttc aagctatggt atgtatgaga ttatttgacc
    10321 ctcatgcagc tataaatata tccactagag aaaatttaga aatgagaagt catataatac
    10381 ctttaggagt aactaaattg agtgcaggtg tatctactga tgtaggaggt cattctcaag
    10441 atgaacatga tactgctcaa tttaagataa atgatgaaag tactgttaaa gatgtggaga
    10501 aaatgctaaa ctcaattggt tatcagcatg tgtttaaaga ttgggaaaga ttttaaatgt
    10561 atctgataac aaacagaaaa ttatgtagtg aggaacgata tttagaagtt attaaagagt
    10621 ctatattgag tggtgtagaa aatataataa taagagaaaa ggatttagaa tatcaagagc
    10681 taagaaaatt atatatgaaa ataaaaacta aaatcaattg tatagatttt caagagcaaa
    10741 tatcagatga aagtttgaaa actaatataa atcaaaaaga gtgtagaaat aaattcaagg
    10801 ttaattttat aataaatagt aacattgaat tttttgaaaa agtggattgt caaggtatcc
    10861 atcttccttt taaattattc ttgaatttaa ttgaaaataa gtataatttt aatgaaaata
    10921 aaatactagg attatcttta cataaagtag aggaagtaga ttatttagaa aaattaataa
    10981 gaaatcaaaa tataaaaata gattacataa cattatcaca tatatatgaa actaaatgta
    11041 aagaaggatt aaatccaaaa ggaattgagc ttttaaaaga agctaaaaag attactgata
    11101 ttaaaataat tgcacttggg ggaattttac catctaatgt caaggaaaca ttaaaatact
    11161 gtgatgattt tgctataatg tcaactataa tgaggtctaa agatattaaa aaaaccatat
    11221 caaattataa tgaaaagttg aattagtaaa tatagtatat tgaattttta acttaaataa
    11281 aaataaaaaa gttatctttt aaattttaaa aggtagcttt tttattttat atctttgcta
    11341 ataaaaaatt gtaaaaaggg aaaactttag tcagagagat taaacaatat cctagaaaga
    11401 tttaaaattt agtaaagtat ggaggacatt ataatgactg acagtaaaaa gaaacgattt
    11461 attataccac acacttttac aataatattt ttactaatag tcttgatggc aatattgact
    11521 tggatagtac caagtggtga atttagtaga gaagatgtag aaggtagaat ggtagttgta
    11581 ccagggactt atcaaagtgt agaaagtaat cctcaaggat ttgcagatgt ttttaaagca
    11641 ccaatagaag gctttatcga ttcagctgaa gttgtaggtt ttgttttaat agttggaggt
    11701 gcttttggta ttgtaaatag gactggagct atagaagcag gaatagcttc ggttatctct
    11761 aagtttaagg gaaaagaact tcttataatt ccaatatcaa tgattttatt tggacttgga
    11821 ggtacaacat ttgggatggc agaagagaca ttaccatttt atatgatatt tgttccactc
    11881 atgacatcct tgggatatga ttctctaaca gcaatagcaa ttgtatttat aggagcagca
    11941 gcaggagccg ccgcttcaac aataaatcca ttttcagttg gtattgcaca ggcactagct
    12001 caacttgctc ctggttcagg agtaacttac agagtagtta tttttatagc atatatacta
    12061 ataagtatag catttgttat gtactatgct aagaaggtaa aggaaaatcc tgaaagttca
    12121 atagtatatg aaatagataa aaataataaa tcaatactta atcaaacttc aacagaaata
    12181 aaagaattta cttttaaaga agtatctgtt cttgctatat ttggaatagg gatggcaata
    12241 atgatttggg gtgttttaag tcaaggatgg tacattccag aaatatcaat gtgttttgct
    12301 gcaataggtg tgctttctgg attggttggt agattatctc aaattgaaat ctcagaaagt
    12361 tttatagatg gagcaaaaga tttagcatct gcagctcttg ctatagcttt ggctcgtgga
    12421 atcgtaataa ttgctcaaaa tggatttata atagatacta tattaaattc cgcagcagcc
    12481 tttctaggtg ggcttccaaa ggttatattt gtatatttat catttttctt ggaagctgca
    12541 ttggcatttt taattccatc atcttcaggt cttgcttcac ttacagtacc tgtacttgct
    12601 cctcttggtg atttggttaa tatatcggct caagtgatag taaccgctta tcaatttggt
    12661 gtgggtctta caaactttat aactccaact tcaggagtac tgatgggagc acttgcagta
    12721 gcccatattc cattttcaaa atgggtaaga tttataatgc ctatcttgtt gattttgaca
    12781 gctgtatctc tagtcttttt aacaataggt atttatatag ggttttaatt aagtgtagtt
    12841 ttatttaaga tatttagtaa tggtataaaa tgataatatg ttttcaataa attgccacta
    12901 atttaattag tggtaatttg ttttttgtat ttaaaaataa atatatttac ttgtattttg
    12961 aattaatatc tacttagaca ttgttttaat tgttatattt tcatttaatt aagcattgat
    13021 ttatcatttt ttatatttca tataacaatg caaaaaataa tttttatata gtaaatgaca
    13081 aaagtttaca ataaatgtat attttcttac tcattcctta aaattactca tttagtttaa
    13141 taactctcat ttcatatgta aagttggtaa aaaaattaaa attaaaagta aaaaactaaa
    13201 taaattaatg ttaatttgaa atatatatga aggtttttgg agaatactta cttatgatag
    13261 gaaaaaatgt taaaataata gtgtaatcaa atataaagga ctgaaataat taacagttat
    13321 taaatattag gagtgtaaaa atttatggga ttaaaagaat ttgattttat tgaagagtct
    13381 attgatatgt tgaggacaat gtcaccaaca ttagaaacta tatcagatga aatagaagag
    13441 tattttgaaa atatcttgga tgaaaagaat caagaatata taaatgtaac ctctagaata
    13501 aaatcagaat caagtttaag agaaaagata attagaaata gatatttaaa aaaatatgga
    13561 gaagcaagta atcttattca taatgtttct gacttaatag gcctaagaat tgagtgtaga
    13621 tttattgagg atgaaaacaa aatatataga cttttgagaa gatattttaa taaaacagat
    13681 gacaaaataa actattataa taaagagaat aagaatatta aattaaaact atctgagaga
    13741 caacccaata agcaaaaaaa tggatttgaa atatataaaa tagatggagt ttttgcgtat
    13801 ttagatagag aagtaaaatt tgaacttcaa attaaatctc ttgtaaatgt gttttggagt
    13861 gaaatagaac ataaaataat ttataaaaac aatacatatt tacttgctga taaatttata
    13921 aaggatatga tggactctat aaagaataat cttactatga tagataatca gcttttaagt
    13981 atttataaaa actttcattc agggaagtct tttaatatga aagtaagtaa aaaagaaatt
    14041 gaaaaattat ttgcgaaact agtctatgac gctttttctg aaaagatgaa taaaagtata
    14101 ggttttgttg tagattttaa aaaaccatgt gagactatat tgaactattc ttttaataaa
    14161 caagatataa gtgatgaagt tttaggtaat tttatgcttg atgagtttgc aagattaaat
    14221 gaaattgtaa acaaggatat agattttaat gagcaaatag aatttgaaag agaaccaaca
    14281 tttgatgata agttttgtaa agacctagga aatcatttta gaagtagatt aaacacagaa
    14341 tttccatgga acctattttt tagaatatta tttgaaatag agccatataa caataccaaa
    14401 gactttgaaa actttgtaga gtttataaaa gaaaatgtgc tactagaaga aaatagggag
    14461 caattattat gccaatttga agaagatagc aaacttataa ttgaagatat atatgattgt
    14521 atatttaata gtataagtga gattgatagt gttgaaattt tatataacta taatcttgaa
    14581 aaaataaact ttacatcaag tgaaatcata aattgtatat gtagagaata tgagtgttat
    14641 ggtgaatact tagaagaaaa agaaaagctt atgaatacat ttaaagaaaa gattattcaa
    14701 atctttgagt aataggtcaa tgctgaagtg gcgctaatgc gccatttttc aaatatatat
    14761 gaattaagta aaattatttt ggaggaggcg atttgtatag agaaaacagg tgtaattttg
    14821 gcagggggaa gaaattctag aatgggtaga gataaagcct ttttagagtt acatgataag
    14881 ctttttattg aaattgcaat agaagcattt aaaaactttg atgagttgat aataatatct
    14941 aataatgaag aattatattc taaatacgat attaaagttt ataatgatat agtaaaagat
    15001 gttggtccaa ttggaggaat atatactgct cttgaatatg caaagtatga tatagttaca
    15061 attgcatgtg atatgccata tctaaataat caaatagtcg aaagaatagc taataaaatg
    15121 aatgataaaa gtgtgatttc agtgacaaat ggaaagttac aaccattgtg ttctggatat
    15181 aaaaagagta taataaataa agtatcttta tgtataaggg aaaatgattt aaagctcaga
    15241 agctttatag ataaaataga taaaagttat atatactttg atgaagaaga tttattttta
    15301 aatgtaaaca ctgttgatga gtatgaaaaa ttaactaaag tttaattagc tatggttgta
    15361 aaatagagta ggatttatat ggaggagagt ttatgaattt acttaaatgc gattcttggg
    15421 cagtaatatt aaataatagc gataaagaaa gtaaagcata taaaatatta gatgaactta
    15481 aaaggaatat gtacaaagtt gttgctatag atgaagagaa aaaacctatt gaaggaatag
    15541 atgtgtatga atgtttaaaa gatgttccac acaatattga tgttgtagct attattgata
    15601 aacaatctaa gatggatgtg attttagaag aagtagagct tttggatata caaaatatgt
    15661 ggtttgaaaa aggaagcttt agtgaaatga tgattaaaaa gactaaagat ttaaaattaa
    15721 atatagaata taatttaagt ttatatgatg agttgactag ataaaattat atatcttaat
    15781 aataaaaata taattagtta ataaaacata ttattttata gtatagattg gttaataaaa
    15841 gtacagattg gttaatgaaa cattaaatta caatatttgt taaaaaaatt gtgagttaca
    15901 tgatataatg tattaaatag gatactaagg agttgatata ttatgttaat agtaactaca
    15961 gaaaaagtag aaggtaaaaa gatatcaaag gttttaggat tagtgagagg aagtacaata
    16021 agagcaaaac atgttggaaa agatatagga gcaagtttta agaatcttgt tggaggagaa
    16081 cttactggat ataatgaaat gctcactgaa gcaagacaaa ttgccatagg tagaatggtt
    16141 gaagatgcag aagctaaagg tgcaaacgca gtaatagcat ttagactgtc ttcagcttca
    16201 gttatgcaag gggcagcaga aatgcttgct tatggaacag cagttgtttt agaagatgat
    16261 aatagtattc ttgaaaaata aaatataaat agttaaaaag aggcatcttt taaaaatttt
    16321 atggatgcct ttttttattt gaaaaaacta atttgagaag aaattttaac tattatgaca
    16381 ataaaattag ggtgatatta tataaataag taaaattata ttatataatg tatggtatag
    16441 actttaaaaa ttttatttgt agagaaaaat atggacaatg ttataataat tatagaaatt
    16501 aaaagtactt acattcatta tgtatgtgac atgtataatt agttttatat ttaatacagt
    16561 tttgaagtaa tatcttcatt ttgagaacgt tggaataata aaaaatgtat tattataggt
    16621 gatataaata aaaaataaat ataatatgtt atattgctga tagaaaagag aataaaagaa
    16681 aataggtt
//
LOCUS       DAEMGG010000089        16600 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEMGG010000089 DAEMGG010000000
VERSION     DAEMGG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.089"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(188..1579)
                     /locus_tag="KPK24_002616"
     CDS             complement(188..1579)
                     /locus_tag="KPK24_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG2300282.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1590..2810)
                     /locus_tag="KPK24_002617"
     CDS             complement(1590..2810)
                     /locus_tag="KPK24_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG2300283.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2824..3612)
                     /locus_tag="KPK24_002618"
     CDS             complement(2824..3612)
                     /locus_tag="KPK24_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2300284.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKNENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3629..4903)
                     /gene="larA"
                     /locus_tag="KPK24_002619"
     CDS             complement(3629..4903)
                     /gene="larA"
                     /locus_tag="KPK24_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG2300285.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(5021..5734)
                     /locus_tag="KPK24_002620"
     CDS             complement(5021..5734)
                     /locus_tag="KPK24_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG2300286.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5859..7118
                     /locus_tag="KPK24_002621"
     CDS             5859..7118
                     /locus_tag="KPK24_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG2300287.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7398..7964
                     /locus_tag="KPK24_002622"
     CDS             7398..7964
                     /locus_tag="KPK24_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2300288.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(8165..9370)
                     /locus_tag="KPK24_002623"
     CDS             complement(8165..9370)
                     /locus_tag="KPK24_002623"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG2300289.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9473..9579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9671..10039)
                     /locus_tag="KPK24_002624"
     CDS             complement(9671..10039)
                     /locus_tag="KPK24_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG2300290.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(10091..11368)
                     /gene="obgE"
                     /locus_tag="KPK24_002625"
     CDS             complement(10091..11368)
                     /gene="obgE"
                     /locus_tag="KPK24_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG2300291.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11479..11769)
                     /gene="rpmA"
                     /locus_tag="KPK24_002626"
     CDS             complement(11479..11769)
                     /gene="rpmA"
                     /locus_tag="KPK24_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG2300292.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11783..12100)
                     /locus_tag="KPK24_002627"
     CDS             complement(11783..12100)
                     /locus_tag="KPK24_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG2300293.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(12115..12426)
                     /gene="rplU"
                     /locus_tag="KPK24_002628"
     CDS             complement(12115..12426)
                     /gene="rplU"
                     /locus_tag="KPK24_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG2300294.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12527..13948)
                     /locus_tag="KPK24_002629"
     CDS             complement(12527..13948)
                     /locus_tag="KPK24_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG2300295.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13966..14667)
                     /locus_tag="KPK24_002630"
     CDS             complement(13966..14667)
                     /locus_tag="KPK24_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG2300296.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14669..16513)
                     /locus_tag="KPK24_002631"
     CDS             complement(14669..16513)
                     /locus_tag="KPK24_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG2300297.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
BASE COUNT         5129 a         3111 c         1763 g         6597 t
ORIGIN      
        1 tttatgtaca catgctttta ggatgacaac atcaacttaa atattatgtt actttctaaa
       61 tttaagtaaa tattgccatc ctaaaaattt tacaatttag cttcagattt aaaagtaaag
      121 tttaaaatat tatatttaaa tctaatttaa tattttttgt tttgtaaata ttattaaatt
      181 aatatttcta atatattttc cctggattta aaatattttt agggtcaaaa gctaacttta
      241 tatttttaat taacatcata taagcatctg aatttgattc atgtaagtac tcttttttag
      301 catatcctat gccatgttca ccagaaactt gaccactaag ttctctagat tttttataca
      361 tatagtcaaa agtttctttt aatcttattt tccatgtatt atcatccata ccatctttta
      421 aaatatatat gtgtagattt ccatctcctg catgaccaaa actttttatt cttattttta
      481 atttgtcttg aagctcgtga gtgtatctta taaactctgc tatcttatct cttggaacaa
      541 cgacatcaca ttcatccatt tgtgtcgtag atgcctttat agcttctaaa aatgcacctc
      601 tggctgacca tatagaatcg tttctttctt gtgtatctga tataaataca tctaatgcac
      661 cattttctaa acataaatta gctacttttt catattcttt ttctatatct tcagtgctat
      721 ttccatcaaa tgtaagcaaa agataagcat ctgatgtatt atctggaaac tttttaccta
      781 aaaattcttc tgctgccaat atcacatctc tttccataaa ttctatagcc gttggtattg
      841 attttgattt tattatttta ggaacagttt ctattgccat tgataaatct ggaaatggta
      901 taagcaaact tatacttttc tttggtaaag gaagcaattt taatatagct ttagtaacta
      961 ttcctaaagt accttctgag cccactaata aatcctttat actatatcca gaactatttt
     1021 taaccacctt tccacctaca tttataattt ctccatttgg tagtacaact tcaagacctc
     1081 ttacataatc ccttgtaacg ccatatttta ctgctctcat accacctgca ttagtgttta
     1141 tattacctgc aattgtagca cttttttcac ctgggtcagg tgggtaaaat aaatctctat
     1201 cttgaacata ttgacctatt gtcatcaata aaacacctgg ttctactgtc aatgttaaat
     1261 tttcataatc aacttcaagt attttattca ttttacatag attaatcatt attccaccat
     1321 ttatggcaac agcagcacct acaagaccag taccttgacc tcttggtgtg actggtatat
     1381 tatttttata tgcgtatttc attattttag atacttgttc agtttcaagt acatttacta
     1441 aaacttcagg gtacttctca atccctccaa gttcatcatg tgaaaaatct tcatttatat
     1501 cacttccaac taatacattt tcttctccac aagtatctat taaaaaatct atatcttttt
     1561 tatcgattaa tttatacatt tctatccccc tatttagctg ctgatgccat atatttttga
     1621 ttattatctt tgttattttc tattttttca atcaatttag gaataatatc ataaatatca
     1681 cctattaaag ctaaatgtgc tacatcaagt ataggcgctt tatcatcttt atttattgct
     1741 actatatagt cagcaccctg catccctgca acaaattgaa cagctccaga tacaccacaa
     1801 gttattatca atttaggttt tacagttcta ccactaagac ctatttgttt tttagcatct
     1861 atccaacctg cttctattac aggtcttgtt cctgccactt gagcgtttaa cttatcagct
     1921 aatttatata tcatctccat atcttcttgt ttttttattg ctctacttgc cacaactatc
     1981 acttcagctt cttccagacc aacttcttta tttttttctt ttacactcag taccttaatt
     2041 ttagatttta gttctttttt ttctacttta cataaagtta tctttcctgt agtattctct
     2101 attttttctg gtgctgaaaa tattttatat cttacagtgg caaattgtgg tctattatta
     2161 ggtgtgttta tatgagccat tatatttcca ccaaatgctg gtcttatttg gtctaaatca
     2221 gtatttgatt gaatatcaag tattgtacaa tctgctgtaa gacctgttct aaatctagcg
     2281 gcaagtcttg gagctaatga cctacctaat gtagttccac caactaatac tatcgttggt
     2341 tttattttat ctataaagtt ttctattgct tttgaatatg gttctatcct aaaatcttta
     2401 aattcatctt catcatatac aaatacttca tccaccccat aagaaatcaa attactgcac
     2461 ttatctttta tgtcttttcc tacaaataca caatacacag gttgttttat ttttgatgcc
     2521 atctcacgag ctttacctat aagctcgtac gttacaggat gtatatttcc attatgatgt
     2581 tcaacatata ctgcaattcc cctccattca tctttattaa tttgtacttt actactctct
     2641 ataaattcaa atgcgccttt tggtccactt ttgacacaca acttgcacat cttacagcca
     2701 gcacttattt ctactatacc tgacttttct tctatagccc caaaaggaca tatttctaaa
     2761 attctcttta agtcagttat tttatcttga tttacaacta tctttgacat ttattactcc
     2821 cccttacacc aactttaatt cctctaattt atttacaagt gcaaaactta gttcatctgc
     2881 atttcctctt acaatatttt tatctgtatt tggctttgga ggaaatattc ttacaacttg
     2941 agtaggagaa ccattaagtc catagttatt ttcattttta tcttcaaagt catctaagct
     3001 atatacaggt atttctctat ctttagtatt aagttttaat ttaaatgaag gtagtcttgg
     3061 ttgaaaaatg tctttttcta cagttattaa acatggatat tcaatctcac atacttgtaa
     3121 atcatttggc atatctattt caacaactat actttcatct ttaacttcaa ttaattttgt
     3181 aacgttagta acatgaggta tatctaaaaa ttcagctact tcaggtccca cctgtgctgt
     3241 atcaccatca gtagtttgtt ttccacaaat tattaggtct acctttccca tcttcttaat
     3301 accttgtgat atggtatagg aagtagccaa tacatctgct cctccaaatc tcctatcact
     3361 tattaatgct ccttcatcag ctcccatcat aaaactttct tcaattacct tctttgcctg
     3421 aggaggaccc atgctaagta cttttagatt cgctttctta tcttccttta ttctaagagc
     3481 tgtttctaaa gcatataggt catagggatt catttttgaa tctactccat ctctttttag
     3541 tactcctgta gtttcatcaa cttctactga agtcgttcca ggaacttgtt taatacatgt
     3601 caaaatttcc ataaagtaca ctcctttttt atttaactat aactgatact ccatcaggta
     3661 taactactac tttgctctcc ttaccaacta tactattcgc catttctaaa gcttctttaa
     3721 agttaaatgc atgtttcata tgcatttttt ttatcatttc aggattacac ttatctgtta
     3781 ccattatcac agtatattta gataatattc tcgctagtat ctgaaattcc cattggtctg
     3841 gaatcgtatc tactctatcg acacttctaa ttttatctaa aagttgtttt ggagaatctg
     3901 catttgctac attttcataa aaagattgac ctccatgacc atcattacat gctgaaatca
     3961 ttattattac tccaccgtct ttacaagtag cttcagctgc tgtcattcct ttaacagatt
     4021 gataaatatt ttggtctagt ggatatccac catttgtagt tacaacaata tcagatttta
     4081 tagaatttac ttttgataac tctgttacaa atctacatcc tttagtatgt gctttttcga
     4141 catcaccagc aaaggaagca attatttttt tatctttatc tattacaaca ttcaatatga
     4201 atgctaattt tgcaacttta gctgcatata ccatatcctc atgaatagga ttattattca
     4261 attttccagt tcttgaatta tcactatcta taaagtcact acagtgattg tacattatag
     4321 tctttgcaga tgcgattcct ggaagtacgc tttttctgcc acctgaaaaa cctgcaaaga
     4381 aatgaggttc tataaaacct tctgctatca aaagttctgc ctcatatgct agtttgttta
     4441 tatataaatc tccacctgaa ggcaatatcc ctactttaca catagattta tcatcagtag
     4501 atatatgcat aactatatct tcattatcta ctatttcttc tcccatttta taaattagct
     4561 cttcccttgt acttggccta tgaaatccag ttgccactat aatcttcaca tcaatttctg
     4621 gattaacatt cctaatcctt cttaatataa ttggcatagt tattctactt ggtacaggtc
     4681 ttgtatggtc acttgtaatt atgactacat tctttttccc cttaaccagt tcttccagag
     4741 aattagagcc tataggatta ttcatagatt cttcaactat ctcttcttga gtcttatgtt
     4801 ctatataatt atccgatttt gactccaaaa caccgagaaa acgttcatca ggtatttcta
     4861 aaatcattcc ttgttttgaa tacggtactt ttaactttac catttttaat ctcccattat
     4921 aataaactat aaaattggta tgtggtcata ccagttttac atttatataa tagtaccatt
     4981 atctaccatt tgcaagaata atttatttta taacaaactt ttaattttca ttttttctat
     5041 aatttttact tataaaatcc atatgttcct ttgctttctc acatgcaagt tctacatcat
     5101 tttctacaac tgcattgtat atagcttcat gctgtttaag caatatattt tcattaaaag
     5161 gttctgcgat tattttttct cttgcatctt ctataaatcc atcaaataat tttgatgctg
     5221 tcataaaaag actttgaatt aaataatttt tacttaatgt tgctagttta ctgtgaaatt
     5281 tttggtctaa taataccagt actcgattat tatctttttt attagtttct accctcattt
     5341 catctattat atgtttcaat tctataactt cagaagaagt tattctcttt gaagcaagtt
     5401 ttgcaatctc aatttctagg attattctaa gctctaatat atcttcaagt gtaccattat
     5461 taagtttaaa aatcatagat aaaggttcta ttaaagtttt ttctatattt gtacatataa
     5521 aatttccttc accctgtctg ctttctatca ctcccatagt ctctaatacc ctaatagctt
     5581 cccttatgga agttctactc acattcattt gttcagacag ttctctttct gatagtaatt
     5641 tatccccctt ttttaactct ccatttagta ttttatattg tatttgctca attacttgtt
     5701 cataaacttt tttactagct atactattaa acattttttc ctccaatatg ataatcgttg
     5761 ttgtaatata tatctattat atatcttaag ccattacatt catatatttt tattactttt
     5821 aatataatat cttataactc aaatttacag gtgatattat gaaaaaaaat aaaattattg
     5881 ctttttttgc tccatctatc atagttggag ttctagtttt agggattatc ctatacccaa
     5941 atgaagctat aaaatctgca agagaaggct tttctatatg gtctaatgtt ttagttccat
     6001 cactactacc atttataatt ggagcaaact taattgttga tttaaaaata gtggatatta
     6061 tagggtttat catcaatccc ataactagat ttgtatttaa tgtttcagga aagagcgctt
     6121 tagcttttgc tatatctaca gtatctggtt atccagtagg agtaagttta gcttctgaat
     6181 ttaggagtaa tggacaaatt tctaagtatg aagcacaaag acttgtgtct ttttgttcta
     6241 catcaggacc tttattcata ataggttcgg ttggtacagg tatgttccaa aattcaaact
     6301 taggttattt aatgattctc tgccattatc taggaactat attggttggt cttttcttta
     6361 gaaattatgg taatgaaaac cttccaaaga caaaattgga tttaaaaaca aatataaaaa
     6421 atgtaatcaa cattagaaat tcaagtggga atggtttttt tgttttattt ggaaatgctg
     6481 tatttaatgg agtaaacaca ttacttacag ttggtggatt tgtaattgtt ttttcagtag
     6541 tatttaaaat actatctctt tttaatgtaa tctcattaat agcatcagtt atatatttgc
     6601 ctctttcatt tttaggtgtt agtaaagaac tctgtcaagc atttgtaagt ggtctatttg
     6661 aaattactat aggatgcaat aaagtatcta gtataacaag ttctccagaa attttaagag
     6721 catctttagc tagttttttg ataggattta gtggattatc catcctagct caatgttgta
     6781 catttctatc aaaaacggac atagatttaa aattatacat actaagtaaa tttgcgcatg
     6841 gagttttagc tgctatcttt atatttatac tttatccaat tgcaaactct gctgtgttgg
     6901 tttctagctt tgctgataca tacaacgtta tatataataa tttaatctgg ttttattacc
     6961 ttagctatta tgatacaatt cttcaaattg tagtaataat ttatctaatc tcagccgtat
     7021 ttattgcaaa aaaacatatg aaaactttat ctacaaatgg gaaactaatg cgatataaaa
     7081 atactttttt cactaagaaa ttttcacgaa aaaaataaaa ctaaaaataa aactaataaa
     7141 acttaactaa atacttccat ttttttatta tatttgtcat aattttaata acctttttaa
     7201 ttagtttaac agcatagtta tttcatcaca atttaataat tttattagaa atatttttca
     7261 aaaaattctc cttaatcaca tatatcacac ttgtataagt ttactgggaa atttttccat
     7321 tatataatat ttcatttata ttatataata aataccgacc taaacttacc aaatcaaact
     7381 aagaggagtg gttgaaaatg gcaacaagac ctatagaaat agacgaatat aaaaagataa
     7441 tggaactatt acatactggc tttacttata gtgaaaatgg cgttgaaaaa agatttagaa
     7501 aaaatcctaa agttgcattg gcactaatgt tagaagctaa tttaggtcta agaatatcag
     7561 atattttaag attaaagata ggtaatttta aaggtaatat gttggaaata aatgaaaaaa
     7621 agacgggcaa attacagtat agaccagtta ataaaaatat aattgagtcc ataaatgaac
     7681 atgcaagaaa gtataatctt aaatcaaatg actacttagt aaatataaag acaaaggcta
     7741 ttcaaaaaca gttgaggata atttgtaaac acttaaatct gtataatata agtacacata
     7801 gctttagaaa gctctatgct actactcaat ttgaaaagag taacaataat ttagagttgg
     7861 ttaaagaact tttaaatcac agctctgtag ccactacaca gcgatatata agggtaactc
     7921 agcaagctat taatgaagct agtgaaaatt tctttattgg ttaattataa cgtatacatt
     7981 tttctattat cttattaatt caccagatat taaaaaagag tgtctcaaaa tgaacttttt
     8041 agttcataag acactctttt tttataaaat caaatatatt tttattattt caacactgga
     8101 aaaaaggctt atctctctat tttgagacac cctattaaat tttcataatt cttgcataaa
     8161 catattaatc tatcttcaat aattttttag ctgattcact gtaattattt ctttttacaa
     8221 tttccaaaac tctatcaatc tcatgttctg actttgcact tacaaaaatc gccaattctt
     8281 catcatctat aatatcataa ctatctacat atttatctaa acattctaaa attccatctt
     8341 tattattata cccaccaaat ctaactactg cactacactc atcttctata agtccactta
     8401 aatccaattt cccttttgaa aaagacttta tatttgaact tctattatct attatatcta
     8461 taatatctgc ataaacagca cttcctgttg cacctcttcc agctccctta cctacaaaag
     8521 aaagttcacc tactgaatca cctactaaaa taactacgtt gaactcatta tctatctttg
     8581 aaagcatatt attattgtcc actaatgctg gtctaacaaa tccacttact tctcctgatt
     8641 catacttact tctagcaacc aattttatct tacaatttaa tttttttgca tattctatat
     8701 ctttttcatc tattgaagtt attccttcta ataataaatc tttccaatat actatttttc
     8761 cataagataa agtagacaat atagataact ttctagcact atcatatcct aaaacatctg
     8821 catctggatt aacttctgca aatcccaact cttgtgcctg ttttaaagcg acatcatacg
     8881 gtaaattttc atcatacatc tttgataata taaaattaca agtcccattt aaaatagcgt
     8941 ttatgctctt tatattgttg ccttccagtg attctatcaa tggttttaaa actggaatac
     9001 cacctgccac agatgcttca aacttgatgc taacattatt taagttagct aattctataa
     9061 ggtcactacc tttttcagcc agtaaatctt tattagctgt cactacatgt atttttttat
     9121 ttaacgctct tgttacataa tcaaaagctg gatttaatcc tccaattact tcaactagta
     9181 tatcaatatc cttgtcaaat atatcttcta tatcagtagt tatcttatca aagtacttct
     9241 tatacttgtg tttttcttta tttttaacta aaattccaac aacttctata tctctttttg
     9301 aagtattatt ttctattaaa tcaattaatc cacttcctac tgttccatac cctaatattg
     9361 ctatcttcac tgtaacatac ccccatcatt ttgattatat taaattattt atatatataa
     9421 atataccaat aaaaaaagct tgagtataaa ctcaagccat atgacgggtt tatactcatc
     9481 tttcagcata aatgctgcag gatttagcac caatgtataa ttaaatatac tggttgccgg
     9541 gcttcacagg gccagtccct ctgccactct tgataagatt gctaatttaa atttatgttt
     9601 aatagtataa tacaatttct tccaattgtc actatttttt taacttattt ccctaaaagg
     9661 atataaacta ttatctaaca gctcttttag ttggtttacc cttttttgta acattattat
     9721 ttttagtctt tgctttcgcc tgttcatgtc ctggaaaaag tattttaggc tcttcaatat
     9781 tcctcttgta aatagtaaat ttaaaaccaa tagcctgcac aaattcagct cttaaagctt
     9841 cacatactgc attagcagtt tctttagtat caagacctgc attttctaat atagtaactt
     9901 ttactaattc tctagttctt aacataccat ctaattgctc taagaaactc tctgtaacac
     9961 cttctttacc aatttgtgta gtaggcttta aagtatttgc caaagatctt aaatacgccc
    10021 tttgttttcc ttttaacata ttctcacctc tataattcta tacttaaatt atctataatc
    10081 tatatttaaa ttaattgtaa aactcaaatt ctaattcata aattttaacc acatctccat
    10141 cttctattcc catttctcta agcctatcaa aaacaccttg actctccata gctttttgga
    10201 aatattgaag ggattccata tcatcaaaat ttacagaata cattattctt ctaagtgctt
    10261 tacctgtaac tacatacaca ccatcttcaa tatctatact taactcttct tcagtaccaa
    10321 tatcaagttc tggtctatac atttcttctt ctgaaactag ctccacatct tctacttctt
    10381 ttagcatttt agaaacatag gctattactt catctatgcc ttgacgagtt gctgctgaca
    10441 ttttaaatac tgtatatcct ctgccttcta attcactctt aaacttctca aacttactct
    10501 catcttctaa tatatcaaat ttatttgcaa ctacaacttg tggtctcttt gaaagctttt
    10561 cattgtacaa tttaagctca tcatttatct tgtcaaaatc ttcaataggg tctcttccct
    10621 ctaatccaga tatatctaca atatgtatca atacctttgt tctctcaaca tgtcttaaga
    10681 actcatgacc taatcctatg ccttcagaag caccctctat aattccaggt atatctgcaa
    10741 gtacaaaact atctccaaac ttagtttgaa caacacctag atttggtgta agtgttgtaa
    10801 aatgatagtt tgcaattttg ggtttagctt tagttacaac tgatagaaat gttgatttac
    10861 ctacatttgg aaatcctaaa agaccaacat ctgctatcat tttaagttca agagttatcc
    10921 atctttcttc tccatctgta ccagattttg caaaagctgg agcttgtctt actgcatttg
    10981 caaagtgttg gttaccttta ccacctatac caccttttgc aactatagct gtatctcctt
    11041 ctttttttaa atctgctaat acaagacctg tagcctcatc tcttattact gtacctgctg
    11101 gtacttttaa tactaaattt tctccatttt taccagctct ttttcctttg cttccgtcgc
    11161 caccattttg agcttgatat tttttctggt acttaaagtc cattagagtt ctaagcccca
    11221 aatcaacttc aaatatgatg cttgctccac gaccaccatc tccaccgtct ggaccaccag
    11281 ctggaacgta tttttctctt ctaaatgcta ccgaaccatt tccaccattt ccagccttaa
    11341 caaatattct tgccttatct ataaacaaat tcttcaccta cttaattaaa aacttttaat
    11401 tagcattacc aaaaatacca ttataaattc atcagtatta taagaaaaag gagtgcctca
    11461 acactccttt tatgtatatt actcagctat agctactgga tatatactaa ctttttttct
    11521 cttcttgtct tttctttcga atttaacagt accatcaact aatgcaaata aagtatcatc
    11581 actacctttt ccaacattag ttcctggatg tattttagtt cctctttgtc taactattat
    11641 acttccagca gttactaatt gaccatcgaa tctcttaaca cctaatcttt tagatataga
    11701 atctctacca tttttagaag aacctacccc ttttttagat gctaataatt gtaaattcat
    11761 gtttaacata tggagtcacc ccctactttt ttaaaatttt tatatttttt gggtattccc
    11821 gtttaatttc tttaaccgct aattgaaagt gatttagtaa taattgtgcg tcttttacat
    11881 gtgtatcttt gactatacac tctatatgac cttcttctcc tactactttt tcaaattcaa
    11941 tcttttgtaa tttgtctaaa gaatttataa ctgcaatagc attagatgta actgcagcac
    12001 atactatatc atagcctatt tcagcaaaat cagcatgtcc ctttaaacaa aaaccttgca
    12061 acaagaaatc atcattataa taatatttaa cttttatcat aacttaacca ataactaagc
    12121 gtttattttt tctacaacta tcttagtgta tgattgacga tgtccttgtt ttcttctgta
    12181 atcttttttt gctttatact tgaaaactat aactttctta gctttacctt gttcaacaac
    12241 tttagcttga acagatgctc cttctactac tggagaacct aattttaatt ctccatcttt
    12301 agagcaagct aatacttcgt ttaaagtaac aacatcacct gcgttagctt ctaatttttc
    12361 aacgaataat acatcacctt cagaaacttt atattgcttt ccaccagtct ttactatagc
    12421 gtacattaaa tacacctcct cggcctcaaa ctcgccagta caaggtactt catttgaaga
    12481 tttaaacctt tactgtgcgg cattacaatt attaattata aaatatttac attttattgt
    12541 caactatttt atcgaaaata acattggttt tattgtgctt taatgtacta tttgcgttta
    12601 aagaaatctt tatattatat ttttcaccaa ttttatcaat tatatccata taatattctt
    12661 ttatttgttc aaaaataaaa tcattaaatt ctaccattat gtttttgtaa acagtatgtt
    12721 cacttattct cataatttct ttttcaatac tatctagtat ataatgtaca gattttattg
    12781 tttgttcacc atcacaacat ggacaagaca ttaaataata cttatcaatg gattcttttt
    12841 ctctacgcct agcaacttct actaaaccaa gttttgtcat acctaatact tcagcttttc
    12901 ttttatcttt attaaattct tcttctagga tgtgtaaaat ctccttttga tgcttctttt
    12961 tatgcatatc aataaaatcg attattatta tcccagctat gtctctaatt ctaagctgtc
    13021 ttacaatttc ttttgcagct tcaagattag ttttataaac ggtttcatca agtttaccag
    13081 ttccaataaa cttacccgta tttacatcta taaccgtaag agcttctgtt ttttcaatta
    13141 ttaaataacc tccactcttt aaccatactt ttttatttaa taatttttct attttagatt
    13201 ctattctgta taaatcaaat acatctctat tttcctcaag acacagttta tcaacataag
    13261 tcttatctat acctcttaaa atagacttaa gttcagagta cttatcataa ctattggtaa
    13321 ttatagattc aatatcatca ttcacattat cttttacata tttagttgca aaatctaaag
    13381 acttatataa taatttcgga cctataccaa gtttatattc ttttaaaatg ttttcatact
    13441 ttaatttcaa ctcttctata tctttcttta tttcatcatg agtgcaacct tgagcctcag
    13501 tccttattat taatcctaga ttttccttat ttactgcctt agtaattttt tttaatctaa
    13561 atcgttcttt ttcatcagta attttatttg atattgtagt tctatcattg gaaggtatat
    13621 agacaatata ccttccagaa atactaatct cagtgtttaa ttttgcgcct ttagttccta
    13681 tttcttcttt atttatctga accaaaatat cctgaccaga ttttatagta cttccctttt
    13741 ttaattgtaa ataagcaagt ttcttaaatc caatatctac aaaacaagct tctattcctg
    13801 gtataacttt ttttacgatt ccacgatata tattggatac agtcttctta tttaaattgt
    13861 cttcaacaaa aagttctgtt aatctttcat cttcaagtac tgctgttttc tgggacccta
    13921 ttagggactc aataactatc tttttcacaa cttcacctct cgagcttaca taggagtcac
    13981 taattcccca tcttctaata catataaatc tcttctcaat atatcaacat ctaatgggtc
    14041 aatatcaagt tcaaacatct ctaatatctt tggtataagt atattagtat ttaaattagt
    14101 ttttgagcct gtagctatag tagcagtaag agtaatatgt tcatcttcta aatttaaaac
    14161 atcaaaggtt cttatcatag gtcttatatc tacttcaacc atcttacctt ttttattctt
    14221 ttttgttatg attatttctt tattattcat aaagtcaatt acttttcttt ttacaaactc
    14281 tttcgtcata ggtcttttca aatctatatt aaacaaatat tctccatagt ctatcacaga
    14341 agaaagagaa ggtgtttgtc tatcaatttc ttttgctttg ataaaatcta tcccatctgg
    14401 aagttgaatt gatactttat ttaaaaattc ctctactgat aaatcatctt cttctatttc
    14461 aatatctaca tactcacctt gactttctgt ccctaaagcc aaagcattgc cataactcat
    14521 tttagggtga ggattaaatc cttgagaatg tgataagttt atttcagctc ttctaaacgc
    14581 tctttgtaac aatctctgaa ggtcaagatg tgatatatat atcatgtcgc cttccttttt
    14641 aaattttact cttattatct tactcatatt agcaaagccc ccttcctata tcatgtatat
    14701 ttattccaca accattgcag ttatgtctac aatcagaagt gattttttct tttttagcat
    14761 tttcgtcttc tctaattaag aattttttac taatacctac atctatatgg tcccaaggaa
    14821 atacctcttc atagtttcta tttctatgtg cataaaaatc tatagaaaga ttattctttt
    14881 ccatagcttc tttccatata tctaaattaa aatgttctgc ccatccatca aatttagcac
    14941 ctagtctaaa tgcatcatat ataacttttc ctattcttct atcgcctcta gctactactg
    15001 cttccatcaa actagtcttt gaatcatgat aattgtattt tatattgtta tttcttaatt
    15061 tatttactaa atgtctctgc ttattaataa cttcttctgt agtatcttga ccatgccatt
    15121 ggaaaggtgt aaatggcttt ggtacaaatg tagaagtact cactgtaaca ctaaaacttc
    15181 tctttagctt tcctccattt acttttctat aaatatcaat aactttataa gctaatttag
    15241 ctataccatc taaatcttca taagtctcag ttggtaaacc aatcataaaa tacaacttaa
    15301 cactattcca ccccatctca aaagctcttt ctgttgcatt ttccaaatct tcttctgtaa
    15361 cacctttatt tataacatct ctaagtcttt gagttcctgc ttctggagca aaagtaagtc
    15421 cagattttct tacttgttgt attttatcag caatttccat tgaaaaatta tctaatctaa
    15481 gggatggaag cgaaatccca atattattag atgcatattc atctaccaaa taatcagtca
    15541 actctgataa cttactataa tcactcgtac taagtgatga taatgatatt tcatcatatc
    15601 cagtattctt aactaattta tctaatattt ctttaagtct tttaacactc ttttctctta
    15661 ttggtctgta tatcatacct gcttgacaga atctacatcc tctagtacat cctctaaata
    15721 attctagtac tattctatca tgaacagtat ctatataagg tactattagt ttttctggat
    15781 aatctacagt ctcaacatct tttattatcc ttttagtagg atttttagta atcccttctc
    15841 tattaggagt tacactttga actgtaccat cttcattgta cttgacatca taaaaacttg
    15901 gtatgtatac tccttctatg ctagatattt tatataaaaa atcttctcta gtcgttttat
    15961 ttttcttcca ttctttatac tcatttacta cttctaaatt aacctcttca ccttcaccta
    16021 atattactac atctacaaaa tctgctaatg gttcggaatt ataagcacaa ggaccaccaa
    16081 ccattataaa tgggtcttcc aaagttcttt cttcttttaa tattggtata ttggctaaat
    16141 ctaatatatt taagatatta gtgtatgaaa gttcatattg aagtgtaaaa gtgacaaaat
    16201 caaagtttgt tataggctct cttgactcta atgcaaataa tggtatactt ttttctctca
    16261 ttatattttc catatcaact gcaggagaat acactctctc acaaaataca tcttcatcct
    16321 tatttattac atcatataat atatgactcc ctaagtgact cataccaact tcatataaat
    16381 ctggaaagca atgtgcatat cttatcaatt cactattact agtatcttta tgaattgaat
    16441 ttatttcatt tccaagatat ctagcaggct tttctacttt ttttaagatt tttttcaagt
    16501 ctactttatt catttattaa aattcctcca aacaagctct ttatttttat tcttatttat
    16561 aagtaatatt atacacgata aaatattttc tatcaaaaat
//
LOCUS       DAEMGG010000090        16508 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEMGG010000090 DAEMGG010000000
VERSION     DAEMGG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.090"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            22..984
                     /locus_tag="KPK24_002632"
     CDS             22..984
                     /locus_tag="KPK24_002632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300298.1"
                     /translation="MNQINSARNKIEKNNKIYNLLDIKPFKPTYETNKLEAYSTDSPI
                     SDDDSMDEYISNYKFDINLNEIILSLSINQGIYNSKKEHVTSFMISDNNEYIFCTKKA
                     IAYSIDWDKKSVRKKKLSEAKVNDWFLFVDDYLNEEGELVINVIQKLLELNLLPSHYL
                     DDYKLTNSWKSELKNYISYSKYNYNDVSNSLAVLGEKINPITIKNWVTNKRLIGPIYL
                     KTFEKIFKVIDSKLDHTEVYDATKSIRSLHTKIKKSIDTFVSDNFFSSKSDIIKDEVS
                     AVVLSMLGNTNDYVTPVQIREIHSCDKYLSPHLVNKVLDLYSVY"
     gene            1002..4529
                     /locus_tag="KPK24_002633"
     CDS             1002..4529
                     /locus_tag="KPK24_002633"
                     /inference="COORDINATES: protein motif:HMM:NF012493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBG2300299.1"
                     /translation="MNCSELRDKTLDLVRKELLGPGSEDLCENIKYEIITDSPIERYS
                     TGILYPLNNNIDVSNTSEYDGEDNVFTSDDTQNELDRAISMSNETLPSSMGMTFFLKK
                     DAEVINFRVNYATYRNALYTDCHVKYTGDDFYSSNTINEYVELRGSFLYLKQKLTSEK
                     VYNLLKKHDLLDNAEAKFVLYKLANQCPDDENKTGGYVRVPHENLIVSIDMDKNYIKK
                     PISKDLDIVLSKRIYSNDFVSITCMLVNSQSGPKKYNNTIFQPELMVLAEDNNDLEFI
                     DIFTAGSVNTFKKLDSEEISLDLLYRNKKTFATGHGVAIDQDIQNDALIKLCTEFIPH
                     FEVPKLDFNVDELSDVSRELLSMKNLSDISDLSKDKKIYFLYKFVDAYDIWIDDLEED
                     SKDLSDVFKDVAYEHIKNCRDTKSRMLEGINLIKSNDEVYRAFSLMNRAILMQRCHSI
                     HITDRLPGSNNLDYIDYSNDNSAWRPFQLAYILTCLSSIENPDSKHRDLVDLLWIPTG
                     GGKTEAYLGLSAFTIFLRRIRNKENGNGTAIIMRYTLRLLAAQQFIRASILICACEII
                     RKENKSELGKKEISIGLWIGSDQTPNFNDEAEEMLNNLTGNNIKFRSSINSEYELDNL
                     KSKYNKFQLLKCPWCGTKLVKEWDKTSKKAVGDWGYRIKSRPKKFYLCCPEEDCSFED
                     MLPIQVVDEEIYNNPPTLLFGTVDKFAMITWTTEVASLFALDDDNSNLSPELIIQDEL
                     HLISGPLGTMVGLYETALDAMCSYKGIKPKIIASTATIRKAQNQVKNLYNRNVAQFPP
                     PGLTSSNSFFVKEMPLSKKRGRLYVGVIPSGKTQVTVEVRLMSALLQRLKMMENVELN
                     LLDKYWTLVSYFNSIRELGKCSTLLNDDIKDNMERLCKRVSAHPYQSLRNLNEQRELT
                     SRLTSTKINTTLDELSNTLTSNNLTVDTLLASNMISVGVDVDRLNLMVVVGQPKLTSE
                     YIQASSRVGRSNEGLIFTLYNPTKTRDRSHYEQFFSYHQSFYKFVEPTSITPFSEPAR
                     ERGMHAVLISMIRHILGLQANDLASRFSKNMDGVEDLKNFIIDRSIDIFRENNQNADD
                     NIVNEFKSEISSELDIIMDLWHDKAQISDKDNLLHYYKRPSKFDNKIYNNLLIPYDKD
                     VITNEKQTLNSMRNVDKQCTVEIIELEEN"
     gene            4531..6444
                     /locus_tag="KPK24_002634"
     CDS             4531..6444
                     /locus_tag="KPK24_002634"
                     /inference="COORDINATES: protein motif:HMM:NF020926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HBG2300300.1"
                     /translation="MSIKLDDKTHNLRINQATSVFGPGAMLDFIDQTLMTAHPNYWTN
                     TIKIQDDRLQATLRVNELRTPPTIDDRSSVPFVRFPRWYFCPKCRKFMPIEDWEKLYK
                     NAHKDKPMKIPICLTCSTKKSTKQLVPTGIITICEHGHIDDFPWVKWVHFRNKSGRKE
                     ICENPQILMKTGKSNLGLEGIELECSCGAKANMKGAFMRLKSSKSNDYGVPSEYKEMF
                     KCSGNSPWYNKKVNKCTKYSFASQRGASNVYYPKIESSIIIPPYSDTLNSKVLSSKGY
                     DTFKKFLDRAIKKNKLERFMDEDFEDFASDIAKETGFDTDKVSIVLKDLLPFEDSSPS
                     TTTRNQYREEEYEALIGNIDAKSKKTNDFTIEEQNLSDYDIPNFSKIVLVKRLREVRA
                     LTGFSRVNPPDNNIFGEEDIDDSCNDTKVISLRSRDCKKSFYPASEVRGEGIFIEINN
                     DMVKSWISSNPKIQDNASILNSRYNKDINVNDKAYRKITPKFLLLHTLAHLIIRELSF
                     VCGYATSSLRERIYCNNSENEKVMSGILVYTASGDSEGTLGGLVRQGMHDSLPSILNS
                     ALNRALWCSNDPICIDSTGQGRNSLNLGACHSCTLLPETSCEEYNLLLDRSMLIGDMD
                     NRDIGFFKNLILI"
     gene            complement(7150..7893)
                     /locus_tag="KPK24_002635"
     CDS             complement(7150..7893)
                     /locus_tag="KPK24_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300301.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKKIKIKFGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(7915..8229)
                     /locus_tag="KPK24_002636"
     CDS             complement(7915..8229)
                     /locus_tag="KPK24_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300302.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(8233..8433)
                     /locus_tag="KPK24_002637"
     CDS             complement(8233..8433)
                     /locus_tag="KPK24_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2300303.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            8580..9341
                     /locus_tag="KPK24_002638"
     CDS             8580..9341
                     /locus_tag="KPK24_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2300304.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDIK
                     EIIKNDVLTKQSIDELLDIGLSEESIETIYDYFSDSTDNGSNIRLKKLISQKENITIK
                     NNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            9398..9596
                     /locus_tag="KPK24_002639"
                     /pseudo
     CDS             9398..9596
                     /locus_tag="KPK24_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9593..10084
                     /locus_tag="KPK24_002640"
     CDS             9593..10084
                     /locus_tag="KPK24_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300305.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            10176..10928
                     /locus_tag="KPK24_002641"
     CDS             10176..10928
                     /locus_tag="KPK24_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG2300306.1"
                     /translation="MNKSYIPKYTHKFPTNIPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILINNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            10918..12084
                     /locus_tag="KPK24_002642"
     CDS             10918..12084
                     /locus_tag="KPK24_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2300307.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPLRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKYLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQGPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYINNNYVFCNELGIPIDDKRPLRNLKSILNSLNIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(12200..12430)
                     /locus_tag="KPK24_002643"
     CDS             complement(12200..12430)
                     /locus_tag="KPK24_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300308.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(12436..12915)
                     /locus_tag="KPK24_002644"
     CDS             complement(12436..12915)
                     /locus_tag="KPK24_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBG2300309.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFDSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            complement(12915..13130)
                     /locus_tag="KPK24_002645"
     CDS             complement(12915..13130)
                     /locus_tag="KPK24_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBG2300310.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            complement(13462..13811)
                     /gene="ssrA"
                     /locus_tag="KPK24_002646"
     tmRNA           complement(13462..13811)
                     /gene="ssrA"
                     /locus_tag="KPK24_002646"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(13969..14424)
                     /gene="smpB"
                     /locus_tag="KPK24_002647"
     CDS             complement(13969..14424)
                     /gene="smpB"
                     /locus_tag="KPK24_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG2300311.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            15058..15885
                     /locus_tag="KPK24_002648"
     CDS             15058..15885
                     /locus_tag="KPK24_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG2300312.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT         6109 a         2182 c         2296 g         5921 t
ORIGIN      
        1 ttatccaaat ataattacta tatgaatcaa ataaattctg caagaaataa aattgaaaaa
       61 aataataaaa tatataactt acttgatata aaacctttta aaccaactta tgaaaccaat
      121 aaattagaag cttattctac cgatagtcct atttctgatg atgattctat ggatgaatat
      181 atatctaatt ataaatttga tataaattta aatgaaatta tattatctct atctataaat
      241 caagggattt acaactctaa aaaagaacat gttacatcat tcatgatttc tgataataat
      301 gagtatatat tctgtacaaa aaaggcaata gcctattcaa tagattggga taaaaaaagt
      361 gttaggaaaa agaaactttc agaggctaaa gttaatgact ggtttttatt tgttgatgat
      421 tacttaaatg aagagggtga attagttatt aatgttatac aaaagctatt agaacttaat
      481 ttattgccat ctcattatct tgatgattat aaattaacta attcatggaa atcagaactt
      541 aaaaactata tatcttatag taaatataat tataatgatg tatcaaacag tttagctgtt
      601 ttaggtgaaa agattaatcc tataacaatc aaaaactggg taacaaataa aagattaata
      661 ggtcctatat atttaaaaac tttcgaaaag atatttaaag ttatagattc taaattagat
      721 cataccgagg tatatgatgc tactaaaagt atacgctctt tgcacactaa aataaaaaaa
      781 tctatagata catttgtatc tgataatttc tttagcagta aatcagatat aattaaagat
      841 gaagtgtctg cagttgtttt aagtatgctt gggaatacaa atgattatgt tacacctgta
      901 caaatacgag aaatacacag ttgtgataag tatctgtcac cccaccttgt taacaaagtt
      961 ttagacttat atagtgttta ttaaataaat aggaggtaaa tatgaattgt agtgaactaa
     1021 gagataaaac cctagatctt gtaagaaaag aacttcttgg acctggttcg gaagatcttt
     1081 gtgaaaatat aaaatatgaa ataataacag atagtccaat agagcgttat tcaacaggta
     1141 ttttatatcc acttaataac aatattgatg taagtaatac atctgaatat gatggtgaag
     1201 ataatgtatt tacatcagat gatacacaaa atgaactaga tagagctata agtatgtcta
     1261 atgaaacttt accttcttct atgggtatga cttttttcct aaaaaaagat gcagaagtaa
     1321 taaatttcag ggtaaattat gctacttata gaaatgcttt atatacagac tgtcatgtta
     1381 aatatactgg tgacgacttc tattctagca atacaattaa tgaatatgta gaacttaggg
     1441 gatcattcct atatcttaag caaaaactaa catctgagaa ggtttataat ttactaaaaa
     1501 agcatgattt acttgataat gctgaagcta aatttgtttt atacaagtta gctaaccaat
     1561 gccctgatga tgagaataag actggtggtt atgttcgtgt tccacatgaa aacttaatag
     1621 tatcaataga tatggataaa aactatatta aaaaacctat atctaaagat ttagatatag
     1681 ttttatctaa aagaatttat tcaaatgact ttgttagtat tacttgtatg ctggttaata
     1741 gtcaatctgg gcctaagaaa tataataata ctattttcca gcctgaacta atggtattag
     1801 ctgaagataa caatgattta gagtttatag atatttttac agctggatca gtaaatactt
     1861 ttaaaaagtt agattctgag gaaatatctt tagacttact ttaccgaaat aaaaaaactt
     1921 ttgctactgg tcatggtgta gctatagatc aagatattca aaatgatgct ttaataaaac
     1981 tttgtactga gtttattcct cattttgaag tacctaaatt ggactttaat gtagatgaat
     2041 taagtgatgt ttctagagaa ttactgtcta tgaaaaattt atcagatatc tctgatctta
     2101 gtaaagataa gaaaatatat tttctttata agtttgttga tgcttatgat atttggatag
     2161 atgatctaga agaagattca aaagatctta gtgatgtttt taaagatgta gcatatgagc
     2221 atataaaaaa ctgcagagat actaagtcta gaatgctaga aggtataaat ttaattaagt
     2281 ctaatgatga agtatacaga gcattttctc taatgaatag agctatactt atgcaaagat
     2341 gtcattctat acatattacg gatagactcc ctggatctaa taaccttgac tatatagatt
     2401 actcaaatga taattctgct tggagaccat tccagcttgc ttatatactt acatgtttat
     2461 cttcgataga aaatcctgac tctaaacata gagatttagt tgaccttctt tggatcccta
     2521 ctggtggagg taaaacggag gcctacttag gtctatctgc ttttactatc ttcttaagaa
     2581 gaataagaaa caaagaaaat ggtaacggta cagctatcat aatgagatat acactaaggc
     2641 tattggctgc acaacaattc ataagagcaa gtatattaat ttgtgcctgt gaaattatta
     2701 gaaaagaaaa taagtctgaa cttgggaaaa aagagatttc tattggtttg tggattggga
     2761 gtgatcaaac tccaaacttt aatgatgagg cagaagaaat gcttaataac ttaacaggta
     2821 ataacattaa gtttagatct agtataaata gtgaatatga gttagataat ttaaaatcaa
     2881 aatataataa attccaatta ctaaaatgtc cttggtgtgg aacaaaactt gttaaagaat
     2941 gggataaaac ttctaaaaaa gctgttggtg attggggcta tagaattaaa tctagaccta
     3001 agaaattcta cttatgctgt cctgaagaag attgttcatt tgaagatatg ttacctatac
     3061 aagttgtaga tgaggagatt tataataacc ctccaacact tttatttggt acagttgata
     3121 aatttgcaat gataacttgg actacagaag tagctagtct ttttgcttta gatgatgata
     3181 atagcaactt atctcctgag ttaataattc aagatgagct tcatcttatt tctggacctt
     3241 taggtactat ggtaggttta tatgaaactg cacttgatgc tatgtgtagc tataagggta
     3301 taaaacctaa gattatagct tctactgcta ctataagaaa agcacaaaat caagttaaaa
     3361 atctatataa tcgtaatgta gctcagtttc cacctccagg attaacatct agtaactctt
     3421 tctttgttaa agagatgcct ttaagtaaaa aacgtggccg actatatgta ggtgttattc
     3481 catctggaaa aactcaagtt actgtagagg ttagacttat gtcagcatta ctccaaagac
     3541 ttaaaatgat ggagaatgta gagttgaatt tattggataa atattggaca ctagtatctt
     3601 attttaatag tatcagagaa ttaggtaagt gtagcacact tttaaatgac gatataaaag
     3661 acaacatgga aagattatgt aaaagagtct cagcccaccc ttatcaatca ttaaggaacc
     3721 taaatgaaca aagagagctt actagtagac ttacttctac taagataaat acaactttag
     3781 atgaactatc taatactctt acttctaata atttaactgt cgatactcta ttagctagta
     3841 atatgatttc agttggtgtt gatgttgata gattaaattt aatggtagta gttggtcagc
     3901 ctaagttaac atctgaatac atacaagcta gcagtagagt tggtagaagt aatgaaggac
     3961 ttatatttac attatataat cctacaaaaa caagagatag atctcattat gaacagttct
     4021 ttagttatca tcaatcattc tataaatttg ttgaacctac tagtattaca cctttctctg
     4081 aaccagctag agaaagaggc atgcatgcag tattaataag tatgattaga catattttag
     4141 gacttcaagc taatgatctt gcaagtcgat tttcaaaaaa tatggatggt gtagaagatt
     4201 taaaaaactt cataatagat agatctatcg atatatttag agaaaataat caaaatgctg
     4261 atgataatat agtaaatgaa tttaaatctg agatttcatc tgaattagat attataatgg
     4321 atttatggca tgataaagct caaatatctg ataaagataa tttattgcat tattataagc
     4381 gccctagtaa atttgataat aaaatatata ataatctttt aattccttat gataaggatg
     4441 ttattacaaa tgaaaaacaa acattaaatt ctatgagaaa tgttgataaa caatgtactg
     4501 ttgaaattat agaattggag gaaaattaat atgtctataa aattagatga taaaactcat
     4561 aatttaagaa taaatcaagc tacaagtgtt tttggaccag gagctatgct agattttatt
     4621 gatcaaacat taatgactgc tcatccaaat tattggacta atacaattaa aatacaagat
     4681 gataggcttc aggctacatt gagggttaat gaactaagaa cacctccaac tatagatgat
     4741 agatcttctg ttccttttgt taggtttcct agatggtact tttgtccaaa atgtagaaaa
     4801 tttatgccta tagaagattg ggagaaactt tataaaaatg ctcataaaga taaacctatg
     4861 aaaataccta tttgccttac ttgtagcaca aaaaagtcta ctaaacaatt agtacctact
     4921 ggtataatta ctatttgtga acatggtcat attgatgatt ttccatgggt aaaatgggtt
     4981 cattttagaa ataaatctgg aagaaaagaa atttgtgaaa atcctcaaat attaatgaag
     5041 actggaaaaa gtaacctagg attagaaggt atagaacttg aatgtagctg tggagctaaa
     5101 gctaatatga agggcgcatt tatgcgttta aaatcttcta aatctaatga ttatggtgta
     5161 cctagtgaat ataaggaaat gtttaaatgc tctggtaatt cgccttggta taataaaaaa
     5221 gtaaataaat gcactaaata ttcatttgct tctcaaagag gtgcctctaa tgtatattat
     5281 cctaagatag aaagttcaat tattatacca ccatactctg atactcttaa ttctaaagtg
     5341 ctatctagta aaggttatga tacatttaag aaattcttag atcgtgctat taaaaagaat
     5401 aagttagaac gctttatgga tgaggatttt gaggactttg ccagtgatat tgctaaagaa
     5461 acaggttttg atactgataa agtatccata gttttgaaag atttattgcc ttttgaagat
     5521 tcttcaccat caacaacaac tagaaatcaa tatagagaag aagaatatga ggctttaatt
     5581 ggaaatatag atgctaaatc taaaaaaact aatgacttta caatagaaga acaaaatcta
     5641 tctgattatg atattccaaa cttcagtaaa atagtgttag taaaaagact tagagaagta
     5701 agggcattaa ctgggtttag tagagtcaat ccacctgata ataatatatt tggtgaagaa
     5761 gatatagatg atagctgtaa tgatactaag gttataagtt taagatctag agattgtaaa
     5821 aaatcttttt atcctgcatc tgaggttaga ggtgaaggta ttttcataga gataaataat
     5881 gatatggtaa aaagctggat ttcatctaat cctaaaatac aggataatgc atctatttta
     5941 aattctagat ataataaaga tattaatgtt aatgacaaag cttatagaaa aattacacct
     6001 aaattcttat tactacatac tttagctcat ttaattataa gagaacttag ttttgtttgt
     6061 ggatatgcta cctcttcact tagggaacgt atatattgta ataattccga aaatgaaaaa
     6121 gttatgtctg gaattttagt ttacactgca agtggtgact ctgaaggtac tttaggagga
     6181 ttagttaggc aaggtatgca tgattcatta ccatctatat taaatagtgc tcttaatcgt
     6241 gctctttggt gttctaatga cccaatatgt attgatagta ctggtcaagg tagaaattct
     6301 ttaaatctag gagcatgcca ttcatgtact ttattgcctg aaactagctg tgaggagtat
     6361 aatttattac ttgatagaag catgttaatt ggtgatatgg ataatagaga tattgggttc
     6421 tttaaaaatc taattttaat ttagtaataa ctaatttttt atttatataa ctaaaggtta
     6481 aaagttaatt ttatgacttc tatcctttag ttttttgtat ttaaattatt attttttgca
     6541 aatcattctt actagaaatt acttattata actaattccc aataattata taattttgtg
     6601 ctcatactga aatttttaaa taaactatta atttatatta tatatgattt ttcaaaataa
     6661 aaaacatatt aactattaaa tcaatatgtt ttcgtggatt ccaatggtgg agaccgagga
     6721 gaatttataa gtggttattt gattgttttg ttggttttta taagtatagg tatatggcta
     6781 tttggtcatg atgttgaagc aatttttctg gttatttttt attgtctatt tttgtagttg
     6841 gggtattatt ggggtataat tttctacttt ccaaaattat tgatacttca ataatataaa
     6901 ttaaattctt agttaagttt tttaatgaat aggtaaatat tatttgtact cataacaata
     6961 tagtttacgc ataattttat atatattttc tggtatatct gcaactaact tagtgtaatt
     7021 taaacgatat tagagatact taaaaaacaa atatatgaaa gatttataac taacttagtg
     7081 taatttaatt gattgctctg cttctgtatt tttaattaag ttgtttgaat tatttataat
     7141 ataacctcct taccacttta attgtgagtc atctggatta tatattcctg tctctttatt
     7201 tatgacaatt tccttgctat tataatttcc ctctagtatc ttagtaaaat tacttgaatt
     7261 cattatccaa tcaaaatttg ctttccatcc tgtattatta tttccattca gaaaacttgt
     7321 tttactaact atatttataa cctctaatac agtttcttta tcatattctt ttattcttga
     7381 atttattctc ctctttcttg tatctgttag tttcttgacc tttgaaagtt caatacaagt
     7441 tgagttaaat ttgcttatga tttcactata tataatatta ttttcattct ttatattctt
     7501 gattgttgtt gactgattgt taatagtctg ttgattaggt tgttgatttt ctatattctc
     7561 ttgataaagc tcccagtttt caatagttat tactatatac ttgtttgttg tttcgtatgt
     7621 tagaaaacca gcatttttaa aaaccaacaa acaacttcta accatttgat aactaactcc
     7681 tttaccacat ctttcagcta ttttgctata tgaagaaata aattgtccaa actttatttt
     7741 tatcttttta ttatctataa aaacatgatt ttctttatga ctagccataa gtaatagagt
     7801 tattaatatt acctttcttt ctggtttaat atctttccaa attgatttat ccaacaactc
     7861 cctataaagc tttatccacc ctttattgct caattgcaaa cacctacttt aagactataa
     7921 cttgatacca tttgcatttt caataaacca atcatctaat ttatttttta ttatgtatac
     7981 tcgattattt atccatatac aagggaatcc tttgttttta gaatgacata actgcctaaa
     8041 ttttgattct cctataggat aaagcttaga tgcttcttta catgttataa gctcagcttt
     8101 atttcttttc gcattttcaa taattttata tccttgcttt ttttcttgtg cttcttcttc
     8161 attctttaca ataatgttta aagtggcttg taatgtcttt aaaacctcta aattattatt
     8221 ctctttcata ttttaatcct ccttactaaa aaataattct tctactgttg tatctaaagc
     8281 tctagatagt ttaatcatca aatcattgct tggattatta gccttacctg tttccaagtt
     8341 tgataaataa tatcttgata taccaactct atctgccagt tcttgttgct ttagaccttt
     8401 tttaatccta aatattttta aattgattcc catacttttt cctccttttt tctagtttac
     8461 attcgtaaac tttacatttt aattataatg tcgatatatg tgtactgtta ataagaatat
     8521 attatttttc tgttctcatt tgtgtacttt tgggatatac tttatacgag gtgataatat
     8581 tgcaaattgg aaaaaactta aaaaagataa gaaaacaaaa agagctaacg cagattcaac
     8641 ttgctgaaat tagtggcatc tcaagaaatg ctctaataaa ttatgaaaat gataaaagaa
     8701 tacctagtat agatacctta tcaaaactcg ctaaagcttt gaaaattgaa aagactatct
     8761 tttattttga tattgaaaat gaagtaatga ctaatgaaga tgttttatac cttttacagt
     8821 ctttattccc agaagaatac tattcttttg atgaattttt aaatgctatt tcaaatctta
     8881 tggaagatga tattagaatt acctcaagtt tattgctagg tgcctggtct gataaagacc
     8941 aatattataa acttggtgaa aaattagaac taaatgaaaa tcaagtttat aattggatac
     9001 tttcagattg tatatctaat actgaattag atttttcatt acatcctgat gacataaaag
     9061 aaattattaa aaatgatgtt cttacaaaac aatcaattga tgaattatta gatattggtc
     9121 tatcagaaga aagtatagaa acaatctatg attactttag tgactcaaca gataatggaa
     9181 gtaatattcg actaaaaaaa ttaatatctc aaaaagaaaa tattactatt aaaaataatg
     9241 attactttaa attattacaa gaaaataacg aactaaagga aaaaaatgaa aagcttgaaa
     9301 atacacttaa agaaataaaa aaactactaa ctaaaatata aaataatatc aatactatct
     9361 aaagcaatga taaaaaacta tgaatggagt tacaattatg aataaatcat atatgctaaa
     9421 atacacatat aaatccccta ctaatttatt tcatggagat aaaaaatata ctctaacata
     9481 cactaagaga gttatgagta atttagtata tgatatggga taatctagaa aataactcct
     9541 ttacatgttt agaagtacaa tcattgtttg atgatatatt taaggtgaaa ttatgatgaa
     9601 taatacaatt ggagaaaaga ttaaacaata tagaaaatcg ataaatatgt caagggatga
     9661 gctagccaac aagattggtt gttcagttca tgctatagct aaatatgagc aaaatcaaag
     9721 aatgcctagt ttagatatga taagaaaaat agctactgct ttatcaattg ctataaataa
     9781 actcattgtt gaagaaaatg atttagatac tttaacaaat aataatatta atttaaaagt
     9841 atttgataaa gagttggatt tagtaattca catattagaa aattatggtt atgatataaa
     9901 aacagactat aaaaaaaagt ttgaaattac agataatgca gatacaataa aaccaattaa
     9961 cctaaacttc tctgacttca aagacttctc caaaaatcta aattgggtga taaatggtct
    10021 tgttgaaaat tttatacaag aaaactctaa tggtcttatt gaagatgagg atgaagaata
    10081 ttagtattgc acattttcaa aatatgagtt tacctattaa cttttataca ttcatagtta
    10141 ataaataaaa attagatatg aaaggagcta aaatcatgaa taaatcatat ataccaaaat
    10201 acacacataa attccctact aatatacctc atggagataa aaaatatact ttaacataca
    10261 ttagaagaat gataagtgaa tttgtatatg atatgggtaa cctagaaaat aaccccttca
    10321 cattcccaga agtacaaaca ttacttgatg gtataaccgt aggcggacat aaattaagtg
    10381 accaaaatca aatactaaat ataaaatcat catgggatta tgtaataaaa ttaagtaaca
    10441 aagaaaactt atctttaaat aaagatacaa tttgctctat acataaaata gttgcaaaag
    10501 atgaagctct aatcgttgga gatttcagaa atggtaatat aggtatagct ggtactactc
    10561 aatatgaatg catagaagcc actctattaa atgatttatt tgtaaatgac ataaatatca
    10621 taaataaaat aactaaccca ttagaaaagg ctataataat aaatttatgg ttgtcctatt
    10681 gccaattctt ctatgatggc aacaaaagaa ctgctagact tacctcaaac ctaatactta
    10741 taaataatga tataggagtt ctatcaatac cagctaaaca taaacaagag tacaacacat
    10801 taatgctaaa cttctatgaa acactagaag ctgatgaagt tataaaattc ttattagaga
    10861 aatgtattac attctttgat ggatataact ataaatcata taaggagctg tttaaaaatg
    10921 gcaactaaaa gagctaatgg agaaggctct atcggaaaat ataaagatgg ttggcgttcc
    10981 cgtataatga ttggctataa tgaagatggt aaaccactga gaaaagaatt ttatggtaag
    11041 actcaaaagg aagttaaaga taaattagat gcttataaaa aacaatatta tttaagctct
    11101 gatatttcag atgataaaat aactttagag cattggtttt acaattggtt atttgaatat
    11161 agaataaaag atttaaaacc caagtcattt gaaagatatg aaagcatcta tcgtaattat
    11221 attaaagata ctgatttagg caatgttaaa ttaaagtact taagagtatc tcacatacaa
    11281 aaatattaca atagattgct tgattccaat aaaagtatac ctactataag gcaaataaac
    11341 actaaactga aaacctgctt atcagaagct gaaaaacaag gtcttataca acgtaactat
    11401 tgtactatgg taaatatacc agttgagaaa aaagaaaaca aacttgaaat attgactctt
    11461 gaacaacaaa agagttttat aaaagctata gatgggcata aattagaggt attattttta
    11521 cttgctcttg gtactggact tagattagga gaattattag gacttaaatg gtttgatatt
    11581 gattttaaaa agtctaattt aacagttaaa agaactcttc aaagaacata ttttatagat
    11641 aaaactggaa atagagagct taaagtatta gagcaagggc caaagacatc aaattcttac
    11701 agaactgtac ctattccaaa agatgtttta aataaattga aagagcataa agtaaatcaa
    11761 aatacagata tattaaaggc tggagaatta tatataaata ataattatgt attttgcaat
    11821 gaattaggca tccctataga tgataaaaga cctttaagga atttaaagtc tatattaaat
    11881 agcttaaata tagagcctat aaagtttcat ggtcttagga aaacttatgc tactagactt
    11941 tttgagaatg atgttcctcc taaaactgta caagttttaa tgggtcatta tgatatatca
    12001 ataactttga atatatatac tcaagttatg gaagataaga aagttgaagc tgtagaaaag
    12061 ttggataaaa tattttcatt gtaaaaatca aatacatata aatattgtga tttcatatag
    12121 ttaattacaa aatcaaaata aggatacaaa ttaattttat attatattgt atccttattt
    12181 cgtatctagt tttaattatt tatcttggat agcccttatg gtacattaat ccattttcta
    12241 acattaattc ttgagccaag tatatatgac ccttacagtg catatctgat acaccctcac
    12301 ttataagttt atatgtttca gaagtatata aataatccat actttcttcc aagctcttac
    12361 ctgtttgttt ggaaaattct aatatgattt ttttatatag tatttgtaat aatgttttat
    12421 ttgcttccat aatcattata cctcctcttc atacctaaac tttaaatatt tatctattac
    12481 attttgattg gttatacata tttgttcatt tggtttatag tattttaatc tttttaaagc
    12541 ttcatctttt tcaataagat tatcttgata cagttctatt gtattgtaaa tcttatcatc
    12601 agcaatacca cctattataa tgtcatattt catatatata tcactcatat ttctacattc
    12661 tattataaaa tcaagccatt ctaaattata actatcgaag atttttacct tgcagttttc
    12721 ttttaattta tcaatatcta aagaataact atttaaatat gcttttttac ctcttttttt
    12781 aaaacgctct gtccagttaa ttgcttgtgt ctttatactg gttacataga atccttttcc
    12841 aaaatctaaa gaatctcttg aaaaggtaat actaggcgtc tttaccacac aatatgaacc
    12901 atgatataca atcattatat cacacccttt tctttaagta cttgcattaa atcttccact
    12961 atgtaacctt tactttgaga atgtagtgct tcatagcaag gtattatata gttatctaaa
    13021 atatcagtat catgtttaag aagtttataa actccttctg aatctatatt caatttttct
    13081 gctaaagctt caatgcaaaa aatagaaaat tctagttttc tttcatccat tacaaacctc
    13141 aactcctatc aagcttatat aatatatatt tttactaatt aacaacttaa actgtcttac
    13201 atttataaaa atattatatc ataaagtaga tattaaaatt ctatgttacc tattatcata
    13261 ataaacaaat aaaataataa acaaataaaa taataaacaa ataaaaactt tttatacagc
    13321 ctaaatgcac cccaatatac ccccaaaaaa acctcatttt ctgtggttgg ggtacggttg
    13381 gtggatttct tctccaaaat aaaaaatact agtcaacaaa gactagtatt tccaagtatt
    13441 tcatcattct tttttcaaaa ttggtggaga cgaggggagt cgaacccctg tccgcaaatc
    13501 ttttccttgc tgcatctaca tgcttagcca acttcactaa tatctcacct cacatagcgc
    13561 caagtggcag gctctatgct tagccagtcc caattaatct acttcaagtc gagacacccc
    13621 taaagtagtt ccctgcataa tgacgtccag tcttagtcgg caggaggact aagagggacg
    13681 agcagcaact aggctgctat cgcgaaatta tcgtctgcgt ttattttaaa gttccccagt
    13741 ttttacgtga cccagagtaa cacgacatgc agcctcaagt tccaaaccca cgtcgaaacc
    13801 tttacgcccc cttgagtagt ttatagtcca gtagactttt ctcaaacttt atcaccattt
    13861 ttattaaatt tttaatgtat atataatata atacttttaa gaataaaaat caatgttatg
    13921 ttctagataa taaagaattt tattctaaaa aatacagttt tatctagttt aatacttacc
    13981 tgtcatttct ctttctatac gtctttgtgc atctttctta gccatatctt gacgtttatc
    14041 atatagtttt ttaccttttg ctaaggcaat ctctacttta actttaccgt tttttagata
    14101 caaactaagt ggtataagtg aatacccctt tatagtagtt gctccaataa gctttcttat
    14161 ttcatgctta tgaagtaata gttttctaac tcttaaagaa tcaacattga atatatttcc
    14221 ttgctcatat ggacttatat gaacttgttt taaaaatact tcgctattat ctacagatgc
    14281 aaatccatca cttaggttaa ctttgccttg tcttattgat tttacttctg ttccttttaa
    14341 ttcaattcca cattcataag tttcttctat aaaatattca tgtcttgctc ttttgtttgt
    14401 tgctaatatt tttttaccag ccatataaat ctcaccttct ttgctagaaa tttgttccat
    14461 aaatgttaga aatttactaa atatttaatg ttgccaaatt atttgactaa cttgtatgat
    14521 aacatattta ggcattttag tcaatatttc actgatatat taagttagtt aggctatata
    14581 taattaagta atataaatgt atgaaaatag ctaaaaaact acttcgaata tacttaatac
    14641 taaactaact ctaaccagta atactaattt ataaatttca aaacaaaaat attacatatt
    14701 ataaatcttc tatcttctat cttctatctt ctatcttcta tcttctatct tctatcttct
    14761 atcttctatc ttctatcttc tatcttctat cttctatctt ctatcttcta tcttctaaat
    14821 aattttaaca tgcccataaa atataatacc cacaaatgat aaataatact caaaatgtct
    14881 attgaaataa ctatacatcc ttaaataact taacttaaat ctaaacattt caaaaacttc
    14941 ttaaaaatat gtaaaaattt gatgataaag tagcgatttt ttcaaaaaaa atgtaatata
    15001 gagttatatc ttataatatt ttagagataa attttatcat agtggggaga aatttgtatg
    15061 gatttaagca aaaaagtaaa aattttagaa aatgcatcag atggcaaaag cgaatttgag
    15121 atattagaat ttgaaaaact tgagggagct tctagcccat taatggcaat gggattatat
    15181 tttgctagag aagctggttt gaaagctaga caaattaaga taaaattaaa taattcatca
    15241 ataaaaacag aagctggtgc attatactat tataaaggaa atatatcttc tgaaactaag
    15301 attggtggtg ttggaggtct ttttaaaaaa ggaatatcag gagcacttac taatgaatca
    15361 atgatgaaac ctgtatacaa aggtactgga gaagtttatt tggaaccttc ttttagacat
    15421 tatttattta tgagtcttga taatgattct ataattgttg ataaaggatt attctattgt
    15481 tgctctgata caatagaagt aaaagcattt acacaaaaga acttctcatc tgctttactt
    15541 ggtggtgaag gcttcttcca aatagaatta agtggtactg gtgtaatcgt tctggaatgt
    15601 gttgtacctc aaagtgaaat tgtagagtat gaattgaaaa atggtgaaga attaaaggta
    15661 gatggtaact ttgcaatagc aagaacatct ggtgttaatt tttctgttac aaaatcagat
    15721 aaatctttaa ttggttctgc tttaaatggt gaaggtttct taaatacatt tactggtagt
    15781 ggtacagttt ggctagctcc aactcaagta atgtatgaaa aactaagttt tggagcaatg
    15841 cctactaatg ataatatgaa taataaagtt tctaagcaaa aatagttgtt ttataaatat
    15901 ttattaaatc attattgatt ttataaagtc actataaatt ttattagatt attataaaat
    15961 cagagtgtgg aaatatttat gtgtattctt taaataaaaa tttccacatt ctgtaaagtt
    16021 attatacttg atatataaaa ttcaatttag gaacatagtc taacatttat aaacatatta
    16081 aggtaatgat ttctcttggt agttattctc atatcttatt ctttgattat atttatctta
    16141 ttcctagccc ttatgtcata ttatatttct tatcttatta tttataatta gcattaactt
    16201 atctaaaatc ttgtattttt ttaatagtaa agtttacatc tatattaatt tcataaaaca
    16261 atcaaggtag tcaagagatt caactttatt tgattttaaa aatttaagtg aatttgttct
    16321 tatacacacc tttttaaaat tatatctgta gaaaatatca tttagagcta taattaaatt
    16381 aacttattaa attttagaaa atttattaaa aaatataagt taaattaata tttttaaaga
    16441 ttcttttttg taactactag aacactaatt tagtagttat tgtatctcta aagtttaagc
    16501 atggatag
//
LOCUS       DAEMGG010000091        16437 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEMGG010000091 DAEMGG010000000
VERSION     DAEMGG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.091"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..456
                     /gene="tuf"
                     /locus_tag="KPK24_002649"
     CDS             <1..456
                     /gene="tuf"
                     /locus_tag="KPK24_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG2300313.1"
                     /translation="LVGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEI
                     ERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLP
                     EGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGVVATIIE"
     gene            706..855
                     /gene="rpmG"
                     /locus_tag="KPK24_002650"
     CDS             706..855
                     /gene="rpmG"
                     /locus_tag="KPK24_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG2300314.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            881..1102
                     /gene="secE"
                     /locus_tag="KPK24_002651"
     CDS             881..1102
                     /gene="secE"
                     /locus_tag="KPK24_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG2300315.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            1122..1664
                     /gene="nusG"
                     /locus_tag="KPK24_002652"
     CDS             1122..1664
                     /gene="nusG"
                     /locus_tag="KPK24_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG2300316.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1703..2128
                     /gene="rplK"
                     /locus_tag="KPK24_002653"
     CDS             1703..2128
                     /gene="rplK"
                     /locus_tag="KPK24_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG2300317.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            2199..2897
                     /gene="rplA"
                     /locus_tag="KPK24_002654"
     CDS             2199..2897
                     /gene="rplA"
                     /locus_tag="KPK24_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG2300318.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2949..3077
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3120..3626
                     /locus_tag="KPK24_002655"
     CDS             3120..3626
                     /locus_tag="KPK24_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG2300319.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3684..4049
                     /gene="rplL"
                     /locus_tag="KPK24_002656"
     CDS             3684..4049
                     /gene="rplL"
                     /locus_tag="KPK24_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG2300320.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            4441..5229
                     /locus_tag="KPK24_002657"
     CDS             4441..5229
                     /locus_tag="KPK24_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG2300321.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5700..9416
                     /gene="rpoB"
                     /locus_tag="KPK24_002658"
     CDS             5700..9416
                     /gene="rpoB"
                     /locus_tag="KPK24_002658"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG2300322.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9458..12943
                     /gene="rpoC"
                     /locus_tag="KPK24_002659"
     CDS             9458..12943
                     /gene="rpoC"
                     /locus_tag="KPK24_002659"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG2300323.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            13232..13654
                     /gene="rpsL"
                     /locus_tag="KPK24_002660"
     CDS             13232..13654
                     /gene="rpsL"
                     /locus_tag="KPK24_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG2300324.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13778..14248
                     /gene="rpsG"
                     /locus_tag="KPK24_002661"
     CDS             13778..14248
                     /gene="rpsG"
                     /locus_tag="KPK24_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG2300325.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            14297..16363
                     /gene="fusA"
                     /locus_tag="KPK24_002662"
     CDS             14297..16363
                     /gene="fusA"
                     /locus_tag="KPK24_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG2300326.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6450 a         2042 c         3360 g         4585 t
ORIGIN      
        1 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
       61 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      121 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      181 aagtttacag cagaagtata tgtacttaaa aaagaagagg gtggaagaca tacaccattc
      241 tttgatggat atagaccaca attctatttc agaacaacag atgtaacagg agcttgtaag
      301 ttaccagaag gaatagagat ggtaatgcct ggagataacg taacaatgga agtagactta
      361 ataaactcaa tagttgtaga agagggatta agattctcaa taagagaagg tggaagaaca
      421 gtagcttcag gagttgttgc tacaataata gagtaattct ttactaaact gatggattaa
      481 aaggaataac aaaaagaggg gttatgctcc tcttttttta acatagaaat acaaaaacat
      541 tgacacagca taggattttg tgataactta tacaaggtaa tgattatagg aagttaataa
      601 tatcttgaat actgtagatt tttaataaat acatatttta aagatattat attcacatat
      661 attaacaata tatattattg tattacaaag ttggaggtgt atcagatgag agttaaagta
      721 actttagcat gtacagagtg taagcaaaga aactacaaca ctactaagaa taagaaaaat
      781 aatccagaca gaatagaatt acaaaaatat tgtagattct gtaaaaagca tacaactcat
      841 aaagaaacaa aatagttttt tataaggatg tgagtatgga atggctgccc aaaaaaatga
      901 cggtgcaaaa actaaaaaaa ggtttagttt gtttggatac ttaaaagaaa ctaaacaaga
      961 attaaaaaga gttacatggc cgacaaaaaa ggaactgttt aaaaacacaa gtatagtttt
     1021 aactgtagtt atttcatgca ctatattagt atggggtata gacactatat tatctggtgc
     1081 actagcgtta ttactaaaat agacgaaaag aggtaagtta aatgtcagaa ttacaagaag
     1141 caagttggta tgtagttcat acctattcgg gacatgaaaa taaagttaaa gctacaattg
     1201 agaaagcagt taaaacaaga ggtatggaag actgcataag acaagtagtt gttccaacag
     1261 aagaagtagt ggaaactact aaaactggaa aagaaaaaac tagacaacgt aaagtttatc
     1321 caagttatgt tttggttaaa atgataataa ctgatgaatc ttggtatgta gtaagaaata
     1381 ctaagggagt tacaggtttc gttggacctg gttcaaaacc agtgccatta agtgaagacg
     1441 aagtcaaagc tatggggata gacacaacag accctaaagt agtaaatagc gatattgatt
     1501 ttgaaatagg agatacagta aaagtatctc aaggaccatt tagtggacaa ataggtaaca
     1561 tagaagaaat tgacttagaa aatagagaag tcaaagtatg cataaatgca tttggtaaaa
     1621 ggactctatt tgtaatagag cttgaaggta tagaaaaaat ctaatactat attatagaaa
     1681 aatctgagga ggtgcgctca aaatggctaa aaaagttata ggtcaaataa aattacaaat
     1741 acctgcagga aaggctactc cagcgccacc agttggacca gcattaggac aacatggtgt
     1801 taatataatg ggattcacaa aagaatttaa tgctaaaact gcagatcaag ctggaatgat
     1861 aataccagtt gttataactg tatatcaaga tagatcattc agttttataa caaaaactcc
     1921 accagctgca gttttaatta aaaaagcatt aaacttaaaa tcaggttctg gagaaccaaa
     1981 caagaaaaaa gttgctaaaa tgacttcagc tcaagtaaga gaaatagctg aattaaagat
     2041 gcctgactta aatgctgcat cagtagaagc tgctatgagt atgatagctg gtactgctag
     2101 aagcatgggt gttgtaatcg aagactaatt aaaagataca ctttttttta taaagtggga
     2161 ggctagaagc cgaaattaac cacgaggagg aaaataaaat ggctaaaaaa ggtaaaagat
     2221 atgcaggtgc attacaaaaa gtagatagaa ctaaatttta tgatgcatca gaagcattaa
     2281 cattagtttc agatatagct ggagctaaat ttgatgagac agtagaagca cacattaaat
     2341 taggtgttga ctcaagacat gctgaccaac aagtaagagg tgcagttgta ttacctcatg
     2401 gaactggtaa aacaaaaaga gttttagttt ttgctaaagg tgaaaaagct aaagaagctg
     2461 aacaagctgg tgctgacttt gtaggagctg aagaattagt tcaaaaaata caaggtgaaa
     2521 actggtttga ctttgatata gtagttgcta ctccagatat gatgggtgta gtaggtagat
     2581 taggtagagt attaggacct aaaggtttaa tgccaaaccc taaatcagga acagttacat
     2641 ttgatgtagc taaagctata gatgaaataa aagctggtaa agttgaatac agattagaca
     2701 aaactaacat aatacatgtt ccagtaggaa aagtatcatt tggtggagaa aaattaactg
     2761 aaaactttac tgcattaatg gatgctataa taaaagctaa accagctgct gctaaaggac
     2821 aatatttaag aagtataaca gtagcttctt caatgggacc tggagttaaa ataaacccag
     2881 caagaacagc tgaataataa aactgttgac acaggttgac aaaaatgtta ttatatcact
     2941 tgtcaatgta aaaaagaata gatttgccgt agatagtggg tgcgaaagct taatatcccg
     3001 ccgaggtttt agataaattg agattactaa agcttttgat gtctactttt ggcaacaaaa
     3061 gctttttata atgttgcggt aaatactaag cctatttggt gaaggaggtg cacatctata
     3121 tgagaaaagc tatagaaatt aaatcagaag ttgtttctga gatagttgaa aagttacaaa
     3181 aatcttctgc tgctgttgtt gtagattaca aaggattaac agttgaagaa gtaactgagt
     3241 taagaaaaca aatgagagaa gctggtgtag attacaaagt atacaaaaat actttagtta
     3301 gaagagcagc taaagaagtt ggtatagagc aatttaatga tgaattatta gttggaacta
     3361 atgccatagc atttggatat gatgatccag ttgctccagc tagaatatta aaaggattca
     3421 tggattctca tccaaaaatg aaattaaaga tgggtattgt ggaaggtgca ttctatgatg
     3481 aatctaagat tgttgaaatg gctaacatac catcaagaga agttcttatc gctaaattac
     3541 ttggtagctt aaaagctcca gtatcaaact tcgcatactt aatagatgca atagcgaaaa
     3601 aggcagaagg tcaagaagaa gcttaattgc ttaactaaaa aattataaat attaatttga
     3661 aataaaattc ggaggtgcta aaaatgacaa tagaacaaat attagaagct atagaaaata
     3721 tgaaagtttt agaattaaat gaattagtta aagctgctga agaaaaattc ggtgtatcag
     3781 cttcagctcc agtaatggta gctggtgcag ctgctggtgg accagctgct gaagaaaaaa
     3841 ctgagtttga tgtagtatta actgacgttg gttcttcaaa agttggagtt ataaaagcag
     3901 ttagagaaat aacaggatta ggattaaaag aagctaaaga agtagttgac aatgctccta
     3961 agacagttaa agaaggagct tctaaagaag aagctgatca aataaaagag aaattagaag
     4021 ctgctggagc taaagtagaa gttaagtagt ttctttaaaa agatacccat tgggtatctt
     4081 tttttattta taaaaaacta taaagaaagt atttggaatt ataaagaaag tatttttata
     4141 aaataaaaaa aatttatata atagtactaa attaagggta tcatatatga tgtaatggta
     4201 ttttaattta acattattat caaattaaaa tataaacaat tgttgtaatg tctatatata
     4261 aagtatataa ttaaactaaa atgtaaaatt ttacatttta gtttaaaaaa atacacgagt
     4321 gttttgtaaa taaaatgtaa taaataatta attaacaaat ataaatttta aaataagtta
     4381 gatatttaga aaaatcgaaa tttgtaggat ttaaaggaca tattcaagga ggagttaata
     4441 atggaaaaat tacaaggaaa aattgcagta gttactgcag caacaaaagg tattggatta
     4501 gcatcagcag agatattagc aaaaaatgga gcaactgtgt acttagcagc tcgttcagaa
     4561 gaattagcta atgaagttat aaataaaata agtgcagaag gtggttgtgc taagtttgtt
     4621 tactttaatg ctcgtgaaga agagaccttt acctcaatga tagaagaggt agttaaaaaa
     4681 gaaggtaaga tagatatact tgtaaacaat tttggttcaa caaacccttc tcttgacaaa
     4741 gaccttgtaa ctggggatac agataatttc tttgacacag taaatactaa tttaaaaagt
     4801 gtgtatttac catgtaaagc agcaattcct catatgataa agaatggaaa aggaagtata
     4861 gtaaatatat caagtatagg ttcagtatta cctgatttat ctagaatagc ttactgtgta
     4921 tcaaaagcag caatcaactc attaactcaa aatatagcta cacaatatgc taaagacaat
     4981 gttagatgta atgctgtact tccagggctt attgcaacta aagcagcatt agacaatatg
     5041 tctccagagt tcataaaaga atttttaaag catgttcctt taaatcgtat aggggaacca
     5101 gatgatatag caaaagctgt tctattctat gctagtgatg actcatcatt tataacaggg
     5161 gatttacttg aagttgcagg aggatttggt ttacctactc cacaatttgc agataatata
     5221 ttaggataat taaattacta atatgcaatg aaagcttatg cattagttat agtagaaatg
     5281 atatttttat aaaggccata gatataattt atattggaaa ggtttgaggt tcaaaattcc
     5341 aagttaaatt tatttatggt ctttattttg tatattttaa taaattgtat atatttgtat
     5401 aaatgtaatt atttacaatc attttaatat ggaattataa ttaaataggg gggatggtac
     5461 ttgtataaaa ttttttatag aaaattagac ctataaatga aaaatatgga ttttatattg
     5521 gaaaattgta cataatacat aatatatgct taaaataatt tacaaaaaat caaaaataat
     5581 gcatagttta catataagat aaaaaatata ttgaaaaata cttgacatta tgctaaaatt
     5641 attagatgcg ttagaatgaa ctattgtttt atttttaatt attgagaggt gaaaagtgaa
     5701 tgccacatcc tgtcacgata ggtaaaagaa ctagaatgag cttttctaaa attaaggaaa
     5761 tagctgatgt gccaaatctt atagaaattc aagtagattc ctatgagtgg tttttaaaag
     5821 aaggtttaaa agaagtgttt gatgatattt caccaataga agattatact ggtaatctta
     5881 tattggaatt tgtcgactat tctctagatg ataaaccaaa gtacgatata gaagagtgta
     5941 aagagagaga tgctacatat tgtgcacctc taaaggttaa ggtaagactt ataaataaag
     6001 aaacaggtga gataaaagaa caagaagtgt ttatgggtga cttcccttta atgacagaaa
     6061 gaggaacttt cgttataaat ggagcagaaa gagttatagt aagtcaatta gttagatctc
     6121 ctggtgttta ctatgcggaa gaaagagata aaacaggtaa gagattgatt tcatctactg
     6181 taattcctaa tagaggagca tggttagaat atgaaacaga ttctaatgat gtaatatctg
     6241 taagagttga tagaacaaga aaacaaccag ttacagtttt acttagagct ttaggaatag
     6301 gaacagatgc agagataatt gaccttttag gagaagatga gagattatct gctacattag
     6361 aaaaagataa tactaagaca gtagaagaag gtcttgtaga gatatacaaa aaattaagac
     6421 caggtgaacc tcctactgta gaaagtgcat catctttatt aaatgcttta ttctttgacc
     6481 caaaaagata tgacctagca aaagttggta gatataaatt caataagaaa cttgctttat
     6541 gttatagaat aatgaataaa atttcagcag aagacataat aaatccagaa actggagaag
     6601 tatttgtaaa agctggagaa aaaataagct atgatttggc taaagccata caaaatgcag
     6661 gtataaatgt agtaaatcta ctaatggatg atgataaaaa agttagagtt ataggaaata
     6721 attttgtgga tattaagtct cacattgatt ttgacataga tgatttaaac ataaaagaaa
     6781 aagtacatta tccaacttta aaagaaattt tagatggata tagtgatgaa gaagaaataa
     6841 aagaggctat aaaatcaaga attaaagaac ttataccaaa acatatatta ttagatgata
     6901 taatagcttc aataagttat gagttcaata tattctataa tataggaaat attgatgata
     6961 tagatcacct aggaaacaga agaataagat ctgtaggtga attgttacaa aatcaagtta
     7021 gaattggtct ttcaagaatg gaaagagtta taaaagaaag aatgacggtt caagatatgg
     7081 aagctataac gcctcaagcg ttagttaaca taagaccagt ttcagctgca ataaaagaat
     7141 tttttggtag ttctcagtta tctcagttca tggatcaaac aaatcctcta tctgagttaa
     7201 cgcataagag aagattatca gcccttgggc ctggaggtct ttcaagagaa agagctggat
     7261 tcgaagtgcg tgacgttcac cattcacact atggtagaat gtgtccaata gagactccag
     7321 agggaccaaa tatcggtctt ataaactctc taggaactta tgcaaaaata aatgaatttg
     7381 gatttataga atcaccatat agaaaatttg ataaagaaac atcaacagtt actgatgaaa
     7441 tacattattt aactgctgac gaagaagatt tattcgtaag agctcaggca aatgagccat
     7501 taacagaaga tggtaaattc gtaaaccata gagttgtttg tagaactgta aatggtgctg
     7561 ttgaaatggt tcctgaaagt agagtagatt acatggatat atctcctaag caggttgtat
     7621 ctgttgccac tgctatgata cctttcttag aaaatgatga tgccaaccgt gcccttatgg
     7681 gagcgaacat gcagcgtcag gcagtgcctc tagttagaag agaagcacca attataggaa
     7741 ctggtataga atacagagca gcaaaagact ctggagcagt tgttgttgct agaaattctg
     7801 gtatagcaga aagagtaaca gcagatgaaa taattataaa aagagaagat ggaaacagag
     7861 atagatataa tctacttaag tttaaacgtt caaactcagg tacttgtata aatcaaacac
     7921 ctataataaa taagggtgac cagataataa aaggtgatgt tatagcagat ggtccagcaa
     7981 ctgatttagg agaagttgca cttggaagaa actgtcttat agcattcatg acttgggaag
     8041 gttataacta cgaggatgct atattaataa atgaaagatt agttaaagaa gatagattat
     8101 caacaattca tatagaagaa tatgaatgtg aagctagaga tacaaaacta gggccagagg
     8161 aaataactag agatatacct aatgttggag atagtgcaat taagaactta gatgacagag
     8221 gtataataag aataggtgca gaagtagact caggtgatat attagttggt aaagtaactc
     8281 caaaaggaga aactgaactt actgctgagg aaagattact tcgtgcaata tttggagaaa
     8341 aggctagaga agttagagat acttcactta aagtgcctca tggtgaatct ggtataatag
     8401 ttgatgtaaa agtatttact agagaaaatg gagatgattt atctccagga gtaaatgaat
     8461 tagtaagatg ctatatagct aaaaagagaa aaataaaagt tggagataaa atggctggtc
     8521 gtcatggtaa taaaggggtt atctcaagag tattacctga agaagatatg ccattcatgg
     8581 aaaatggaac accactagac ataatactta acccacaagg tataccatca cgtatgaaca
     8641 ttggtcaggt tttagaagtt catttaggac ttgctgctaa gacattagga tggtatgtag
     8701 caacatcagt attcgatggt gctaatgagt atgacataat ggatgctctt gaagaagctg
     8761 ggtatcctag agatggtaaa ttaactttat atgatggtag aacaggtgag tcttttgaca
     8821 acagaataac tgttggatat atgtactact tgaaactaca tcacttagta gatgaaaaac
     8881 tacatgcaag aagtactgga ccatactcat tagttactca acagccacta ggtggtaaag
     8941 cacaatttgg tggacaaaga tttggagaga tggaggtttg ggctctggaa gcatatggag
     9001 ctgctcacat acttcaagaa atacttactg ttaagtctga tgacgttgta ggtcgtgtta
     9061 gaacatatga agctatagtt aaaggtgaaa atatacctga accaggaatc ccagaatcat
     9121 ttaaggttct tataaaagaa cttcaaagtt tatgcttaga tgtaaaagta ttgacagatg
     9181 aagaccaaga aatagaagta agagaatctg tggatgaaga tgatacaata ggtgaatttg
     9241 agctagatgt tgtaaatcat atgggagaag ttgaagaaag taatataata gaagaaattg
     9301 aagatgattt tgcagaaaat gcagaagatg aggatataga gaatttagaa gaattcactg
     9361 aagatgattt atttgaagaa gaaatagatt ttgatagtga tgactttgat atgtaaattt
     9421 aattaatgta aatctcaaat agaagggaga gaactccttg tttgaattaa acaatttcga
     9481 gtcgataaaa atagcattgg cttctccaga aaaaataaga caatggtcta ggggagaagt
     9541 taaaaagcca gaaactataa attaccgtac tttaaaacca gaaaaagatg gtcttttctg
     9601 tgaaagaata tttggaccac aaaaagactg ggagtgccac tgtggtaaat atagaagagt
     9661 tagatataaa ggtgtagttt gtgatagatg tggagtagaa gtaactaaat caaaagtaag
     9721 aagagagaga atgggacata tagagctagc tgctcctatg tctcacatct ggtacttcaa
     9781 aggtatacca agtagaatgg gacttttact tgatatgtca ccaagatcgt tagaaaaaat
     9841 attatacttt gcctcatatg tggtagttga tccaggagag actggattaa acgaaaaaca
     9901 attgcttaca gaaaaagaat acagaactgc tcttgaaaag tatggatata cttttactgt
     9961 aggaatgggt gctgaagctg taaagacatt actacaaaat atagatttag agcaacaaag
    10021 taaagaccta agagcggagt taaaagatag tacagggcaa aagaaagtta gaacaataag
    10081 aagattagaa gttgtagagg catttaaaaa gtctggaaat aaaccagaat ggatgatttt
    10141 agatgcaata ccagtaatac caccagattt aagaccaatg gtacaacttg atggtggaag
    10201 atttgcgact tcagacctaa atgatttata tagaagagtt ataaatagaa acaatagact
    10261 taaaagatta ttagagcttg gagctccaga tataattgta agaaatgaaa aaagaatgct
    10321 tcaagaagct gttgatgcat tgatagataa tggtagaaga ggtagacctg taacaggacc
    10381 tggaaataga ccacttaaat ctttatcaga tatgttaaaa ggtaagcaag gtcgtttccg
    10441 tcaaaactta cttggtaagc gtgttgacta ctcaggacgt tctgttatag ttgttggacc
    10501 agaacttaaa ttttatcaat gtggacttcc aaagaagatg gcattagaat tattcaagcc
    10561 atttgttatg gataaattag ttaaagaagg ttatgcacat aatataaaaa gtgcgaaatc
    10621 tatagtagaa aaagttaagc cagaagtttg ggatgtttta gaagatgtta taaaaagtca
    10681 tccagttctt cttaaccgtg caccgactct acatagatta ggtatacaag catttgaacc
    10741 aatcttagtt gaaggtaagg ctataaaact acatcctctt gtatgtacag cttataatgc
    10801 agactttgat ggtgaccaaa tggcagtcca tgtaccttta tcagtagaag cacaagcaga
    10861 agcaagattc ttaatgcttt ctgtaaataa tatacttgct cctaaagatg gttcacctat
    10921 aactactcca tctcaggata tggttttagg ttgctattat ctaacaatag aagcccaaga
    10981 tggagctaaa ggaacaggta tggtatttaa agactttaat gaactattac ttgcttatta
    11041 caataaatca gttcatctac atgcattagt gaaactgaag gtaacacttg aagatggaag
    11101 aagttcattg gttgaaagta ctgttggtag atttatattt aatgaaaata tacctcaaga
    11161 cttaggtttt gtagatagaa aagaaaatcc atttgcactt gaagttgatt tcttagcaga
    11221 taaaaaatct cttggtaaaa taatagataa atgctttaga aaacacggaa atacagaaac
    11281 agctgaatta ctagattata taaaagctct aggatttaaa tattctacat taggtggtat
    11341 aacagttgct gttgatgata tgagtgtccc agaagaaaag aaagtattta tagctgaggc
    11401 agaagctaaa gttgataagt atgaaaaagc atacagaaga ggtctaatct ctgatgaaga
    11461 aagatatgaa aaagttatag agacatggac agaaacaact gataaagtta ctgatgctct
    11521 tatgggtgga ctagatagat taaataatat atatataatg gcacattcag gagccagagg
    11581 ttctaaaaac caaattagac agctggcagg tatgcgtggt cttatggcaa atgcatctgg
    11641 taaaacagtt gagataccag ttaaatctaa tttccgtgaa ggtttatcag tactagaata
    11701 ctttacatct tcacatggtg ccagaaaagg tcttgccgat acagctatac gtacagctga
    11761 atctggatat ttaacaagaa gacttgttga tgtaagtcaa gatgttattg taagggaaat
    11821 agattgtgga acagaagata ctacagagat ttatgctata aaagaaggaa atgaagttat
    11881 agaagagata tatgatagaa ttgtaggaag atatactata gaccctatat taaatcctga
    11941 aactggagaa gttatagttg aggctgattc aatgatacaa gaagatgaag cagaaactat
    12001 agtagcttta ggaattgaaa aaattaggat aagaacagtt cttaactgta aaactaatca
    12061 tggagtttgt tctaagtgtt atggtagaaa cttggcaaca ggaaaagaag ttaatatagg
    12121 tgaagcagtt ggtataatag cagctcaatc tatcggtgag ccgggtactc agcttacaat
    12181 gcgtacattc catactggag gagttgcagg agctgatata actcaaggtc taccaagggt
    12241 tgaagaatta tttgaagcta gaaaaccaaa aggattagct gtaataactg aagtatctgg
    12301 tagagttgaa attgatgaaa caggaaagag aaaagaagta aatgtaatac cagaagaagg
    12361 cgagactcaa acatatgtaa ttccatatgg ttctagatta aaagttaagc aaggtcaaat
    12421 gctagaggct ggagaccctc taacacaagg atttataaat cctcatgata tagtaagagt
    12481 aaatggtgtt aagggagttc aagaatatat agttaaagaa gttcaaagag tgtatagact
    12541 tcaaggggtt gatgttaatg ataagcatat agaagttata gtaagacaaa tgctatctaa
    12601 agtaaaagtt gaagacccag gagatacaga tttattgcca ggtggatatg aagatgtatt
    12661 aacattcaat gaatgtaata aagatgctat agataaaggt ctaagaccag cagttgctaa
    12721 aagagtttta cttggtataa ctaaagcatc tcttgcaact gattcattct tatcagcagc
    12781 ttctttccaa gaaacaacaa gagtattaac agaggcagca ataaaaggta aagaagacca
    12841 cttaatagga cttaaagaaa atgttatatt aggtaagtta ataccagcag gaacaggaat
    12901 gaagaaatat agaaatatag ctgttgaaaa aattgaagat taatagcata tagagtaaag
    12961 aaatcttgac aaccattatt tgtggtgtta aaatagtaaa gtgtgttgtt taaaagtaca
    13021 ctaagggctt gaacaagttc ttagtgactt ttagatggca ataaatgaga actaaaaagc
    13081 atcaagctag aaaagtgcta atatcacata tttatgtgtt atagacatta aattgtaagt
    13141 aacgaatgtt acaatcagtc agtatgaata ttcatattga tgtagttggc ggtcactgat
    13201 aagatcgaaa atttggaagg aggtgcagat gatgccaaca attaaccaat tagttcgtaa
    13261 aagtagaaaa gcgctagaga agaaatctac tgctccagca ttacaaaaag gttacaactc
    13321 tttaaataag aaagtaactg acgctagtgc cccacaaaaa agaggggttt gtacttcagt
    13381 aaaaacagtt actcctaaga agcctaactc agctttaaga aaagttgcca gagttagatt
    13441 aactaatggt atagaagttt ctgcttatat accaggagaa ggacacaact tacaagagca
    13501 tagtgttgtt cttataagag gaggaagagt aaaagacctt ccaggggtta gataccatat
    13561 actaagaggt actctagata cagcaggtgt tgataagaga agacaatcaa gatctaaata
    13621 cggtgcgaag agacctaaag aagctaaaaa gtaaaaacaa ttaatgattg attatagttc
    13681 ggtgctttaa atatatatat atttacggca tcacggcatt aaatgattta tgtcgagtac
    13741 ctatgattta agttaaataa ttaaggaggg aagcgaaatg ccaagaaaag gtaatattcc
    13801 aaaaagagaa gttttaccag atcctatgta tgggagtaag gtagttacta aattaataaa
    13861 taatctaatg gtagacggaa aaaaaggaaa gtctcaaaga atagtttatg atgctttcgc
    13921 aattgttgct gaaaagacag gagaagaagc tttagaagta tttaataaag caatggataa
    13981 tataatgcct gttttagaag taaaagcaag aagagttggt ggggcaaact accaagtgcc
    14041 aatcgaagtt agacctgaaa gaagacaaac attaggttta agatggctag taaaatatac
    14101 tagagctcgt ggagaaaaag gaatggttga aaagctagct aaagagataa tggacgcagc
    14161 taacaacact ggagcttcag ttaagaaaaa agaagatact cataaaatgg cagaagctaa
    14221 taaagcattt gctcattaca gatggtagga tatatagata tcttataaaa ttgaggatat
    14281 cataaggagg ataaccatgg ctagaaagtt tcctttggaa agaactagga atataggaat
    14341 catggctcat atagatgcag gaaaaactac tactacagaa agaatcctgt tttatactgg
    14401 gcaaactcat aaaataggag aaactcatga aggagcttct caaatggact ggatggagca
    14461 agaaaaggaa agaggtataa caataacttc tgccgctact actgcttcat ggaaagacca
    14521 tagaataaac ataatagata ctcctggaca cgtggacttc acagttgagg ttgaaagatc
    14581 tctaagagtt cttgacggtt cagttgctgt attctgtgca aaaggtgggg ttgagcctca
    14641 atcagaaaat gtatggagac aagctgaaac ttatggagta cctagaatag cttttgtaaa
    14701 taaaatggat atattaggtg cagatttcta caatgttgta agcatgatga aatctagatt
    14761 aaattctaat gcagttccta tgcaattacc aataggtaaa gaagattcat ttataggtat
    14821 aatagactta ttaaaaatgg atgctgttat atacaaagac gacctagggg ttgaaatgga
    14881 agaaacagat ataccagaag atatgaaaga attagctgct gagtggagag aaaaattagt
    14941 tgaatcagta gctgaaactg atgaagaatt aatgatgaaa taccttgaag gtgaagaact
    15001 tactatagat gaattaaaag ttgctataag aaaagctact atagcttgtg aaatgaaccc
    15061 agttttctgt ggtacagctt atagaaacaa aggtgttcag ttagtaatag atgcagtttt
    15121 agattactta ccagcgccaa ctgatatacc agctataaaa ggtatattag cagatggaga
    15181 agaagctgaa agacattctt ctgatgaaga gccattctct gcattagcat tcaaaataat
    15241 gactgaccca ttcgttggaa aactagcgtt cttcagagtt tattctggaa cattagaatc
    15301 aggttcttat gttttaaatg caactaagaa taagagagaa agaattggtc gtatattaca
    15361 aatgcatgct aatacaagag aagaaataac taaagtgtat gctggagata tagcagcagc
    15421 agtaggatta aaagatacta ctactggaga cacattatgt gaccctgcta atccaataat
    15481 acttgaatct atggaattcc ctgagccagt tatatctgtt gctatagaac caagttctaa
    15541 agcagctcaa gaaaaaatgg gtatagctct tcaaaaatta gctgaagaag acccaacttt
    15601 cacagttaag actgaccaag aaacaggaca aactataata tctggtatgg gtgaattaca
    15661 cttagagata atagtagata gattattaag agaatttaaa gtagaagcta aagttggtgc
    15721 accacaagtt gcatatagag agactataac tcaaccagtt gatgttgaat acaagtactc
    15781 taagcaatca ggtggtagag gacaatacgg tcacgttaag attagagttg ctcctcaaga
    15841 gccaggtgaa ggatacaaat tcacaaacaa aactgttggt ggatcagtac caaaagaata
    15901 tgttggtcca gttgacatgg gtatacaagg agctatgcaa tctggtatag ttgctggata
    15961 tccagttgta gacgttgctg ttgagttata tgatggttct taccatgagg ttgactcttc
    16021 tgagatggcg tttaagatgg ctggttcaat ggcattcaaa gatgctatga agaaaggtaa
    16081 tgcagttctt ttagagcctt acttcaaagt agaagttgtt actccagaag attacatggg
    16141 agatgttatg ggtgacttaa actcaagaag aggattaatc caaggtatgg aagctagaag
    16201 tggtgcacaa gttataaatg cattcgttcc actttcagaa atgtttggat actcaacaga
    16261 cctaagatct tcaactcaag gtcgtgcaac ttacacaatg atattcgatc actatgagca
    16321 agttccagct tcagttgcta agaaaatagc tgaaggaaaa taatttaaaa ataattaata
    16381 atataaatat aaaaaataat cagctagatg agagatatct ctcatctagc acaagat
//
LOCUS       DAEMGG010000092        15947 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEMGG010000092 DAEMGG010000000
VERSION     DAEMGG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.092"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            555..1205
                     /locus_tag="KPK24_002663"
     CDS             555..1205
                     /locus_tag="KPK24_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2300327.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            1532..3598
                     /locus_tag="KPK24_002664"
     CDS             1532..3598
                     /locus_tag="KPK24_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG2300328.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            3615..5570
                     /locus_tag="KPK24_002665"
     CDS             3615..5570
                     /locus_tag="KPK24_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG2300329.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            5586..6776
                     /locus_tag="KPK24_002666"
     CDS             5586..6776
                     /locus_tag="KPK24_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG2300330.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            7925..8299
                     /locus_tag="KPK24_002667"
     CDS             7925..8299
                     /locus_tag="KPK24_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2300331.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            8301..9158
                     /locus_tag="KPK24_002668"
     CDS             8301..9158
                     /locus_tag="KPK24_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300332.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            9161..9799
                     /locus_tag="KPK24_002669"
     CDS             9161..9799
                     /locus_tag="KPK24_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG2300333.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            10805..11539
                     /locus_tag="KPK24_002670"
     CDS             10805..11539
                     /locus_tag="KPK24_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2300334.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            12335..13591
                     /locus_tag="KPK24_002671"
     CDS             12335..13591
                     /locus_tag="KPK24_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG2300335.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            13654..14847
                     /locus_tag="KPK24_002672"
     CDS             13654..14847
                     /locus_tag="KPK24_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG2300336.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGIYLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            15048..15428
                     /locus_tag="KPK24_002673"
     CDS             15048..15428
                     /locus_tag="KPK24_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2300337.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT         5884 a         1763 c         2791 g         5509 t
ORIGIN      
        1 atagtacact agcatgattt gaagtacaac gatttaatct atagtacact agcatgattt
       61 gaagtacaac gatttaatct atagtataat agtttaattt gaagtacaac gatttaatct
      121 atagtacaat aacttgattt gaagtataac gatttaatct atagtacaat aacttgattt
      181 gcaatacaat gaattaatct atggtataat agtttaattt gaagtacaat gaattaatct
      241 atagtataat agtttaattt gaagtacaac gatttaatct atagtataat agtttaattt
      301 gaagtacaat ggtttaatct atagtataat agtttaattt gaagtacaac gatttaatct
      361 atagtacaat aacttgattt gcaatacaat gaattaatct atagtataag agcatgattt
      421 acagtacaat aatttaaatt gtagtacaaa agtactatca aataagaatt tctactttaa
      481 aaatattact aaaagtaatt aaagtccagt aattcaatat aatatggcaa aagaatagaa
      541 ataaggtgag agtagtgaaa gtaaagtcaa atttagatga aattttatat cataagatta
      601 ttgaaagtct tattcgtggt gaatactctg taggtcaaaa aatattacta aatgaccttt
      661 gtgaaaaatt tgaagtaagc agaacaccag ttgtacaagc agtaaaaatg ctaaataaag
      721 atggagtgct tactattatg acaaatggta aagtgtacgt accagagtat gaatatgata
      781 tggtaaaaca agtttgtgaa acgagaacac tgattgaaac atatgcttta gaaaaaatga
      841 tgcaagaaga ggaagaggtt tttcaacaaa agctagatgt aataaaaaga tactctgata
      901 aatgcgagga gtactatcgt caagaaaaat ctgtagaact tgcactagca gatttggaat
      961 tacataaagc cattgtagaa ggggcaaata atgagatttt aaatgatgta tatgttggta
     1021 tacaaggaag atttatagta gtgaactatt taattcgacc attaaaaaat cgtaattatg
     1081 aaggaacagt acaagaccat tttgaaatat taaaattcat tgaagacaga gatacaaaaa
     1141 aagcagtaga taaactaaga aatcatattc aaggaactat taaaagattc tgtgaagatg
     1201 aataataagt tttgaagtta ctatatatac ttatctatta agttgctaaa tgtagtctaa
     1261 gtataatcta attatagttt aaacataatc taaatataat ctaataaata taatctaaat
     1321 atagtgtaaa taagtctaag tatgaattaa atatagatta agtatagatt aagtatagat
     1381 taagtatgaa ttaagcatga attaagcatg gattaaatat actttaaatt acaaatatta
     1441 caaggaagcc agaaggcttc ctaaaaaaat aacctttata aaatttttta ttttaaattt
     1501 aaaatttttt attttaaatt taaaataaaa aatgaaagag gtggtagaga gtttgaaaaa
     1561 aattactaaa gaaaggatag aaaatactat gaaaaagcta tttacgagtg aaacattttt
     1621 ggaatatcaa tttataagtg attgccaaat atcaccagat ggaggatata cagcatttat
     1681 tgtaaaaaaa gctgatatca aagagaatgg ttacacaagc caagtttata tattaaacaa
     1741 taaaactggc gaattaaaac aaataacgtc tataaactct gttggagcat atgcctggga
     1801 agatgaaaat actatcttgt tccctgcttt gcgaaatgaa aaagtaaaag aagctgtcaa
     1861 aaacggtaag caatgcatga gttactatgc tttatcttta aatggtggag aagcagaaga
     1921 actttttaga ctccctatca aaggtggtaa attgaatcct ataggaaaag gtttgtatgc
     1981 agttattgat tcttatgaca atgaccgacc tattgtagag ggattgccag aagaagaaca
     2041 acagaaaaaa ataaatgctt ataacaaaag acattatgaa catttcaaag aaattcctta
     2101 tgctgtcaat ggggagggat atattagcag aaaaagaaaa cgcctatatg tatacgacag
     2161 taatataaat gagttgaagg caattacagc acctatgttt aatgttgtag gtatgaaaat
     2221 aagtgatgga aaaatcttat atatagggca agagtttaaa gatgtaaaag gtttgaaaaa
     2281 tggggtctat gtatttgata caaacacaaa cacaaatgtc tgtatattag aaaaagataa
     2341 gtatattata aaaggttttg aattatatca aaatcaagtg atactaaacc taactgatgc
     2401 tttatcttat ggaaatggag aaaatggaga tttttatact attgatattg atacgaaaga
     2461 aatgaaactt ctttcagggc atcaacatca ttgtattgga aatacagtaa catctgatgt
     2521 taaaatgggt gctggtcaga caacaaaggt agatggagaa tatatttatt acacttctac
     2581 agtagatatg gactgcatta ttgaaagaat acatattcca acaggaaaac aggaaaaagt
     2641 aacacagaca ggttcagttg attttataga tgtaaaagat ggcaatattg tatgtgtggg
     2701 ttgtttagga aatggattac cagaagttta tacagtagag aatggaacat tgtgtaagaa
     2761 aacacattta aatgaccata ttttagagga atataaaatt tctgtgccag aatacataga
     2821 gtcaaaagga agttctaaat gggaaataca aggatacgta tataaaccag tagattatga
     2881 ggtggggaaa aaatatccag ctgtattggc gattcatgga ggaccaagat tgacatatgg
     2941 accatatttt atgcatgaga tacaggtatt tacaagtgca ggttactttg tattcttctg
     3001 taacccaagg gggtctgaag gtcgtggcaa tgcttttgca gatattagga aacagtttgg
     3061 agatattgat tatatagatt ttatggaatt tacagatact gtacttgaaa aatatacaga
     3121 cattgataaa acaaaattgg ctgttgaagg tggaagttat ggtggattta tgacgaattg
     3181 gataattggt catacaaacc gttttgctgt tgcttgtgct cagcgttcta ttgcaaactg
     3241 gagtggcatg gagggtacaa cagacattgg ttattatttc tgcaaaggac aaacaggtgc
     3301 ttctcatatg gaaaaccacg agcttcaatg gaaacagtca cctcttgctt atgctgataa
     3361 atgtgttaca ccgactttat ttttacatgg tgaaaaagac tatagatgct atatgcaaga
     3421 agcattccag atgttttcag cattgaaaat tcatgggtgt cctacaaaac tttgtctatt
     3481 tgaaggagaa aaccatgaac tgtctcgttc tggtagacca aaacaaaagc tacaaagatt
     3541 agttgaaatg ctagattggt tttcagtata tataaaaaaa gataacatag gacagtagaa
     3601 aggagaatat aaaaatggaa aaaataaaga ttgaaagttc agatattttt gaatatatat
     3661 tccctcatga tatttcttgt tcacctgatg gaaagcatat tgcttatatt atatcaaata
     3721 tcaacgagga aaaagactgc tatgaacatg acttatatgt gatggacatc aaaacagaaa
     3781 aacaaataca tatgactcaa acaaaagatg taacatcatt tagttggatt tctaatacag
     3841 aactcctttt tacatctaaa agaaataagc caaaatcagg tacaacagat ttttacacta
     3901 tttctataga aggaggagaa gcaaaaaaag cattttctat tccaaaagct tgtagcgtac
     3961 ctgtatcact tggaaataaa ttgtggttac tgacaacaaa aaatccaacg gattctaaaa
     4021 aatcagaacc agatagggca gtagagggtg tagattattg gacatttaca gataaacctt
     4081 ttataagaga tggagaaaat ttttctcaaa gaagacgtgt aacattagaa ttatatcaag
     4141 aaggtgaaaa tataacaaaa gccattacac caaaattttg tgaagtagca ggtgtagacg
     4201 tttcttctga taaaaacaga attttatata ctggtcagat ttatgaagat tgtgcaactc
     4261 ctttttctgg actttgggag tatcatatag acagtggtga aaccaaagaa cttgtaccac
     4321 aaggaaagta ccaaataagt ttggcaaaat atattggcaa agataaagta atgttgcaag
     4381 cttcaacatt agaccgttct atcacacaga atcatgatat ttttatactt gatttgagta
     4441 ctaatgaaat aaatatgatt gctagtccag atggtatgta tgctacattg ctggatgtag
     4501 atgcagtata tggtggtgga cgtagtaata aagttatagg agacaaattt attggtgcaa
     4561 gaatttgcag aactatgaca gaatttaatg agtttgatac aaagacaggt aatatacgta
     4621 tcatcacaaa agtagatgct tttacatctt ttgatattta cgacaacacc atgtacacag
     4681 taatgttgaa agattatgag ttagcagaaa tatatagcat tgatatgact acaggtacaa
     4741 tgaaaaaaat gactgctttt tctaaaccat atttagatac tcataaggtt tcattacctg
     4801 aaaaattgac gtttgtagca aaaaataaag aagaagttga tggttttgtt ataccaccta
     4861 tagatgctaa agaaggcgaa aaatatcctg ctgtattgtt cattcatggt gggcctaaat
     4921 gggcatatgg ctatatgttt acacacttga agcagtgtgt aacatcaaag ggaatgtatg
     4981 tgatttattg taaccctcat ggtggtgacg gttatggtga aaaattctta gaaatggtag
     5041 aaagatgggg gtatgttgac tatgagcatc ttatggaatt tgtagatact tgtattgaaa
     5101 aatatcctgg gattgatgca gaccgcttag gggttgcagg gggaagttac ggtggttata
     5161 tgacaaactg gataattggt catacagacc gtttcaaagc agcagtttct caaagaggta
     5221 ttagtaactt gattacagct tctttaatta ttgactttgg ggatagaatt atgaagcaga
     5281 cttgtggaga taagacgcct tggaatcatg aggaggttct ttggaatcat tcacctatta
     5341 aatatgtgaa aaatgttaag acaccaacat tgttcttaca tagtgatagg gattatagat
     5401 gttttatggg ggatactttc caaatgttta cagcattaaa acagcttggt gtagatacag
     5461 aaatgtattt gtttcatgga gatacacatg gattgtctag aaatggaaga ccatctaata
     5521 gaattgcaag agcaaatgct attgtagatt ggtttgaaag atatttataa taagggggtt
     5581 attctatgag tgatatgtta ttatggttta gtgtcgtatt agttttattt gctattgggg
     5641 atttaattgc aagcaaaaca aaagcaaaag tatcagctgt atttgttaca ctacttttat
     5701 ttttgattct atttgtcaca aaagcaatac cagctgatat tattgaaaaa gcaggtatga
     5761 ccgcagcagc tagttggagt gttccaatga ttatgtttag tatgggaaca atgttgaatg
     5821 ttaaacaatt tatagacgaa tggagaacag tattgacagc atggcttgga attgtagcag
     5881 ttatagtctg tgtctcatta tgtattcctt tgtttggaaa atcaacagta ttgacaagca
     5941 taccagttat aaatggagct ttacctgcta ctactattat gacacaagca gcactagaaa
     6001 aaggactgac acttgcagca gctacagcaa cagttgtatt tgcaattcag aagtttattg
     6061 gtacaccaat agcttcaaga gcagcgttac aagaggcaaa ccgtctttta gtagaatatc
     6121 atgaagcaaa atcaaaaggc attgatttag caaatgttga tactgataag aaagaagcag
     6181 agaatacagg cacaaaagta aaacaagctt tctgtgaaaa atacgataaa tattattcta
     6241 caaatgtttg tatatttttt atagcattat tctcttatct agggtatgaa ctttcagaga
     6301 ttattcatgt aaattattct attgtatgtc ttgttgttgg tgttattgta acacgtattg
     6361 gtattgtgcc aaaagacatt ttggaaaaag gaaaaatcaa gggatttatc aacatggttg
     6421 tgtttgcagc tgttattcct tctttagcaa aagtttcttt gacagattta attagtcttt
     6481 ttgtaccaat tgtgggtatg tttgcagctt ctatcattgg tatattcctt atgatgaagg
     6541 tgttaccagg ttggaaaatt atcggttcta aacctcttgc ttttggagta gggttttgtc
     6601 agatgttggg attcccaact acatatttaa tttcaaatga agtgtgtaat gctgttggag
     6661 aaacggaaga agaaagagca tatttgatgt ctaagattat gcctaagctg gtagttggtg
     6721 gtatggcttg tatgatttct attgtggtag ctggtcttat ggtaccaatg ttgtagatag
     6781 gtttcatagt taaaaaagtg gtattaattt ataggagaag ctaaaaagta gtattaattt
     6841 ataaaataat tttatattag ttaatttgtt ataagaatat tttcttttta taattataat
     6901 tttgatttta taagatagca gattttttat ttgctatgct aatagttata atttgttttt
     6961 atttgctaaa atgcaacttt aatttgattt atgtataata tagggtattt aataatattt
     7021 ttgaagaatt tttataaaaa attaaaaaga ggagaaaaaa gggagtgcca caaagtgaac
     7081 tttttagttc taaggcactc ttttttgtac gaaatcgaat atattttcaa cgttttaaaa
     7141 gtgagaaaga gacttatctc tagtttgaga tagccaattt taattattaa aattattttt
     7201 tgttttattt atcaaagagt attcgtgttt tagaagttag tattttttat tttatatata
     7261 caaggttttc cgtttctaat gttttctttt ttaaaataat tagggtattc aattatagta
     7321 aaaggtggat ggtattttgg agtgatatta tttaaagcac agtattgaat gttgaaatcc
     7381 tcacatttta gagtattatc ttttgtaatc aaaaaaatct tctatactta tagcaaggtt
     7441 acttctgcaa atattccata cataataata tcccaattta aattttttta atatagagtt
     7501 tacctctagt ctacactctt gaataactct ttcctctatc caaattctta taaattcatc
     7561 ccatttatta ctatttattt attattatct aaatttttta gtatgtttgt aattagaaat
     7621 ggatttattc ctttatgttt tttataaata gtttataact taatttatca ttaaacaaat
     7681 cattcataca ggattacttt ttcttttata tattcaattc ttacttttaa taaatattta
     7741 atacatccta tcttatgtag ttttaaaaag tgtttatggc tattaacaat ctctaatatt
     7801 tcattcatat ttattttgtc atagtaatat tttacatctt ttatattgac aaaaaagtgc
     7861 tctaagtgta tagtgtgtat atgataacat cacactgaaa gtgataaaaa gggggtgata
     7921 aaaaatgaaa gtgctcattt ctaacacatc agataagcca ttataccaac aaattaaaga
     7981 gcaaattaaa gaagcaatat ttactgaaga actgaaagaa ggggatgcaa tgccatccat
     8041 aagaaatttt gctaatgatt taaaagttag tattttgact attagacgag tgtatgaaga
     8101 acttgaaaat gaaggctttc ttacacgcca agttggaata ggaacattta tttcaactgg
     8161 aaatttagaa atactaagag actcaaagag acgtatagtt gaacaaaaaa tggcagatat
     8221 gattaaagat gcaaaagcac taggaattac acagatagag ttacaagaaa tgatggatat
     8281 tctatatgag gaggattaat atggaaaata ttttagaagt ttataactta aataagactt
     8341 ataccgattt ttcattatct aatataagct ttgcattacc agaagattgt ataaccggat
     8401 ttataggtgt taatggagca ggaaaaacaa caacaatccg ttcgatattg gatgtcgtaa
     8461 agcgtgatag tggaaaaata aaaatctttg gtaaagattt cgatggaaat gagaaggaat
     8521 taaaaaatcg aattggagtt gtctttgatg attcatattt ctatgaggag ttgtcaatgg
     8581 cagaaatgaa aagtattatt gcaccttcat atgataattg gtgtgaagaa gattatcaac
     8641 aaaatatgaa gcgttttaat cttaacccaa aacaaaagat tgatacactt tcaaaaggaa
     8701 tgaaaatgaa atttgcttta gcattagcat tatcgcataa agctgagttt ttaattatgg
     8761 atgagcctac aagtggttta gacccactaa tccgtagtca gatacttgac atactaacag
     8821 gatatatgaa gaaatgtggt cgtggtgttt tcttttccac tcatataaca tctgatttag
     8881 acaaaatagc agatatgatt gttatgattg acaatggacg tattatatta gagcgtgaaa
     8941 aagatactct attggacgaa catagaaaag taaaagggga tattcaattt ctaaatgatg
     9001 aaaccaaaaa attattttta aatttagaaa ttacaggtta tggttttata gggattacct
     9061 caaaaattaa tgaagtgcaa aaaaatatgc cagatatttt aatagagcgt cctacaattg
     9121 aagatattat gcttgcatat attgaaagga gagagtaatt atgttgattc atttagttaa
     9181 aaaagatatt ctgattgtaa aaaaatatgt attgttgata ttatttattg caataggaat
     9241 cccattattt atattatggc gtttacctcc atttgcacaa atattgggat ttgtactatc
     9301 aattatattt tcagaattta tgctgtgtca gtatctatta ttaaaggaga gtcaatattc
     9361 aaaagcatct gtattacttt gttcaactcc ttatcctaga cgtggcttag ttcaatctaa
     9421 gtatgtttta tttgcaatgg tatttatcta ttgtacatta gtttactgga ttgaaaattt
     9481 acttattcca caaataggaa cttttgactt gacatatatt ttgattgttt tattaataag
     9541 ttctattatt tatggagttt atatgcctat tcaatacaaa ttaggatatg aaacaacaaa
     9601 attctttttc gtgatgataa tcatggcttc atcatttgta ttaccattaa ctatagctca
     9661 cagcaatttt gaaattccat ttatatatca gtatccatcc atgatagtga atattgtatt
     9721 gttcctttta agtttaatta tactgttagc ttcaatgttt atttctatta aaatttttag
     9781 taataaggag cttgtataag gtaaggggga cttttatatg ccacatttac cagattgggc
     9841 tgttttactt tgtatcttac ttccaatagt atataaagct tgtcagatat ttaaaagtaa
     9901 aaaatagagt atatatttat attgccattt ttattgagtg aagttagatg gtatgaaaag
     9961 gttacttgaa aataaagtta cttggtgtgg gagagtaaca tcataatatt atagattata
    10021 tgtttcttgt gaatcaattt gtatttttta atagggaata tccagtaatt ttgttttcca
    10081 ttgattattt ttcaattatg agtatgattg tgtgtattgt ataccatctt ttaaggtgtt
    10141 tttaaaaaat tatatcgtca aaatataaat ctaaggaatc ctataaataa agctttttaa
    10201 ttttagaaaa ctaataaaaa gtaggacgtt aataaaaaga agctgtctca aaatagagat
    10261 aagcttcttt ttcattgctg aaatgacgaa aatataattg atttaatata aaaaagagtg
    10321 ctttatgaac tatttatttt gaacagtaga tacacttgca ctcaatttag ttactcctaa
    10381 gctttatgaa ctaaaagttt attttgaagc ttatttttat taaaagagat tttatttttc
    10441 taaataatca tctatataca gtattgcgtt tcttttattt attcataatt tattttctta
    10501 ttaaagtttt tctatattca tattttctat attactttac ttttaatttt atacgaatta
    10561 tcaaaaataa aatagttatt agaaaaaaat aaaagtaatt gcaaagcttg caattactta
    10621 cttctatatt aattactata ccataaaata acaaaaaaat caagtcgagt aatccttcaa
    10681 cttaaatgat atactaggta ttaataattt ttaattaata aaatataact agaggaaaat
    10741 ctacattttc tcaatttctt taattaaatt gacctctaat taagtaatta taaggagaaa
    10801 aattatgaat ttagcaaagg gattagatgt tttaaaaatt tcatcaaatg tattcggtga
    10861 agataaagtt atgtatattc ctgtaatcta cacagaagat gatgcaacac ttattgacac
    10921 aggtcttccg ggtcaaggtg atttaataat tgatgctctt aataaaagta acacgagctt
    10981 tgataagtta aaaaatataa taatcactca tcatgatata gaccatatag gaaacataaa
    11041 ttatctaaga gaaaaatcta agaataatat caaagtatat gcatacaaaa gtgaaatcag
    11101 ctatataaca ggagaagaaa ctccttttaa actttatatg ttggaacaaa tggtagataa
    11161 aattgatgat aagatgctta gtatgctaaa tgttatggga cttggattta aatcatcata
    11221 tactaaagta gatgtgtcat tagataacca tgaaaaattg aatctaggtg aggaaataga
    11281 agttatacat acaggaggtc acactagagg tcacatatgt ctttatttaa aagagtctaa
    11341 agtacttatt gctggagatt tgttacaggt agaaaatgga gaacttaagc ctgttgatgt
    11401 gatgtacagc aacaaacaag agttaaaaga tgctattaaa aatataagta attatgatat
    11461 agaaacgata gtttttagtc atggtggatt atatcaaaga aacataattg gaacattgaa
    11521 aaacctaatt attgagtagt actatatgat ttacaataat ttcttaaatt acttaaaatt
    11581 atatctaaag atgtagaatc aaaattaaat ataagtaata agaattttgc tttaaaatta
    11641 tacttaagtg ttaagggtga tatgtagtat tacaagtatt ttagaatata attttttata
    11701 gtaattttaa agttctacaa tacttcagtt gaaaaatatt taacagcata gtataatagg
    11761 aaagaaaata ttttaaataa actttagtat acaaagtgaa gaaaaatatt taaatcatgt
    11821 atcataatgc aaagtaaaga aaatcattta aattatatat cataaaaata atatgaagta
    11881 ttgaacactc ataatattag agactttaat attttaattg ataggttata ttatttataa
    11941 cattaaaaat acaatttaca taaaatagac atatgaaaaa taaaaaatta tataataatt
    12001 atgggaatga ggttctccct tggatgataa tatcctaaac tgccaatgag ctgatgactt
    12061 ctatgatatc atagatgcca ccagcttttt ttgttgttta aatcgataaa atggaggtga
    12121 agtacttact aaatttttat tttttaaatt tgaagaaata tttaattaat agaaaaaata
    12181 gtagattata tgttaattat tttattaaga atgggttaag agtgtaccta tatcctatca
    12241 atttaaaaaa caattatgat agtcattaca ttgactacag ttgatgtaat aaaactttca
    12301 aaaaatctta tgatgcaaat agaaaggaat taaaatgata gaaaaattta aaactttaat
    12361 atggattaaa atgataataa gaattaaaag cgtttataaa acatatggtg gactattttt
    12421 tttaggactc ataggtatac cagttggtgc aattataggt cttatagata ccatatttgg
    12481 aacggttctt ctaaaagtta cagatattcg agaaacatat ccaatgtatc tgataccatt
    12541 tttagcagta gtaggagttg ttattgctta ttgctatttt aaatttggag gaaaaagtag
    12601 taaaggaatg aatctaatat ttgaggtagg gcatggagaa gaagaaatta ttcctttaag
    12661 attggttcca tttataatat caggtacatg gttaacccat ttatttggtg gtagtgcagg
    12721 acgagaagga gttgctgttc agattggtgc aactttttca cattgggtag gtaaaaggtt
    12781 gcctataaaa aatgcttcta gtatttttct agtaactggt atggcagctg gttttgcagg
    12841 attatttgag acaccaatag cagcaatact atttgctatg gaggttttgg ttgctggttc
    12901 actggagtat caatcgcttt ttcctgcgtt tactgcttct tttacagcaa gtgcagtatc
    12961 aaaagcttta gggttagaga aattttcttt tgcactttct tctaaagttg tatttgactt
    13021 gtcaatattt tggaagctta ttgttttagg aattattttt ggaatggttg gtggagcatt
    13081 tgcatggtgt ctgaaattgt caaaaagaaa aatagggaat agattaaaaa atcctatgat
    13141 aaggattgct attattggag tatgtcttag tgtattattc cttttatttt ataaaggaag
    13201 atattcaggt ttagggacaa atttaattca aaatagtttc tatggtggag aaatttattc
    13261 atttgactgg ctgttaaagt ttatacttac aatactgact ttgtcagcag gttttcaagg
    13321 aggagaggtt acacctttgt tttccattgg agctagtctt ggtgtattgt tagcaggatt
    13381 ttttaatttg ccaattgaat tagttgcagc gcttggctat gctagtgtct ttggtagtgc
    13441 tacaaatact ttttttgcac ctgtatttat aggggcagaa gtttttgggt acagctattt
    13501 accttatttc tttgtagtat gcgctatttc atatatattt aatatggata aatctattta
    13561 ttcactacaa aaaatttcaa caaaacaata aataaaacaa cataatgtat gattagaaga
    13621 gaaagaatat tagagaaagg atagggtagg aatatgttga caagtttagc tttgattttt
    13681 ttattaggaa tggcctcggg aggtattttt aagaggataa aattacctaa cttattggga
    13741 atgttactta cgggaattat attaggtcca tatgttttaa atttgattga taattcaata
    13801 ctagatattt cttctgacct tagaaagatt gcattgatta ttatattaac aagggctgga
    13861 ttatctttag atataaacga tttgaagaaa gtgggaagac ccgctgtact tatgtgcttt
    13921 attccagcta cttttgaaat tatcggaatg atagttttag caccaaagtt actaggcgtt
    13981 tctatattgg aagcagcagt catgggtgca gtagtaggag cagtttctcc cgcaattatt
    14041 gtacctaaaa tgcttaaatt aatggaagaa ggttatggta cagaaaaaag tattccacag
    14101 atgcttttag caggaacttc tatagacgat attttcgtaa ttgtgatgtt tacagtattc
    14161 acaggattgg cacaaggaaa tagtatatca gctattagct ttttacaaat tccagtatca
    14221 attatattag gtgtaatagt tggagcagtg attggaatct atttagcagt attttttaag
    14281 aaggttcata tgagagattc tgctaaaggg gtgttgcttt taagtatctc atttttaatg
    14341 ataagtttag agactgcttt agagggtatt gtgccatttt ctggattatt agcagttatg
    14401 agtattggga ttttccttca gataaaatat cgtgtagttg caagaagatt gtctataaag
    14461 tattcgaaac tatgggttgg tgctgaaatt ctcttgtttg ttttggtagg ggcaacagta
    14521 gatataagtt atgcatttaa agcaggaatt ggagctgtaa ttttaatatt tggagttctt
    14581 ttattccgta tggtaggagt attcttctgc cttattaaga caaatttgac aataaaggaa
    14641 agattgttct gtatgattgg ctatattcca aaggctacag tacaggcggc aataggaggt
    14701 gtaccacttg caatggggat ggcatctgga caacttattt tgacgttagc agttctagct
    14761 atacttataa ctgcacctct tggagctttt ggaattgatg taacttataa aaaattatta
    14821 acttctgtaa aaggcgagaa taagtaaaaa taaagattat tttaactggt aagttatatt
    14881 ttggaaaagg attatactat tgaatacaat ataaaaaaat catttgggag attaagaagg
    14941 tagaagttaa gaacagattt taaggtatgt tatgattatt agaattgatt ttgtgaaatt
    15001 attattgtaa tatttaatta tatagataaa gaagcggagt gataactatg tgtttaaaaa
    15061 aattatcaaa attagagtta gttattatga aatttatttg gaatttagat ataaaaacca
    15121 attcatatga gattattgat tatatgaaag aagaacataa tttaccagaa aaagtagctt
    15181 taaaaacttt atcaaaacta tcaaagaaaa gattcttata tgttcaagaa acaggcaagt
    15241 gtatgtatta tacagttgca atcaaagaaa aggcatattt agagtttata tcaagaaatg
    15301 tgcaaaatct tttgaagaat aattttataa ggaatttatt agtatcattt cacgaagagg
    15361 agctaacaga ggaaaagata aagtcgttag aaaactgggt agtaaattgg gaagaagctt
    15421 atgtataaat gttattattt ttattacaat ttagataatt ttggttttgt aagctctgca
    15481 actattttta gatggttgca gagtttattt ttatttttta ttcccttaag caatacttaa
    15541 aagtaaacgc atttatattt actaacttca attacttcta atacttaaat tgctttttaa
    15601 aaatattttg atatgtcatc tctatagtaa tctagaactt gtttctattc tttatataaa
    15661 gtttttatga atgatatata taatattcgt tcatttgatt aaaaataaca aaatattaaa
    15721 taattctact ctgatagttt gttaaaaaaa taataaaaaa tattaataaa caaaaaaaaa
    15781 atatcttaag agaggagaat gttctaaaat ataaaaaggt ttctagattt cataaaagat
    15841 actattttag tcttgaaaat atttagttta aaaagatttt aatttaatga ttgattaagt
    15901 taaaaatgtg tatgtaataa attttatttt atttattgac taaatta
//
LOCUS       DAEMGG010000093        15776 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEMGG010000093 DAEMGG010000000
VERSION     DAEMGG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.093"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..872
                     /locus_tag="KPK24_002674"
     CDS             <1..872
                     /locus_tag="KPK24_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG2300338.1"
                     /translation="SAPKNLKLLKETLDIDEITFNDCLKFDEITKLDLFDNYDFLSLN
                     TFELRDGEAVIEEVNMYLSDNFILVVVNEEHFLFEFVKNIILKNSQLEKNPVINLFKI
                     NYLILREVIKNGFESLEKVEELILQIEDEMMDNINKNHVSRISDVRGLTRIIVKNTRP
                     LLYIGDRIVKENIRYLKYSNVKKYNLENFQGIDFGIDKLYNFALSTRELADKLLDIYS
                     SRVGEKTNNLITKLTLLTAISAPLTIITGIYGMNFRYMPELNWIYGYPATLFFMLCII
                     FVGIIIFKIKKLL"
     gene            1049..1204
                     /locus_tag="KPK24_002675"
     CDS             1049..1204
                     /locus_tag="KPK24_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300339.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            1334..2347
                     /gene="aroF"
                     /locus_tag="KPK24_002676"
     CDS             1334..2347
                     /gene="aroF"
                     /locus_tag="KPK24_002676"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG2300340.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            2369..3433
                     /locus_tag="KPK24_002677"
     CDS             2369..3433
                     /locus_tag="KPK24_002677"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG2300341.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            3433..4746
                     /gene="aroA"
                     /locus_tag="KPK24_002678"
     CDS             3433..4746
                     /gene="aroA"
                     /locus_tag="KPK24_002678"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG2300342.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELNKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            4724..5803
                     /gene="aroC"
                     /locus_tag="KPK24_002679"
     CDS             4724..5803
                     /gene="aroC"
                     /locus_tag="KPK24_002679"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG2300343.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRVIPVIEAVTAIGIFDLMKGR"
     gene            5806..7002
                     /gene="pheA"
                     /locus_tag="KPK24_002680"
     CDS             5806..7002
                     /gene="pheA"
                     /locus_tag="KPK24_002680"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG2300344.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRFIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            7049..7858
                     /gene="aroE"
                     /locus_tag="KPK24_002681"
     CDS             7049..7858
                     /gene="aroE"
                     /locus_tag="KPK24_002681"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG2300345.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            7870..8403
                     /locus_tag="KPK24_002682"
     CDS             7870..8403
                     /locus_tag="KPK24_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG2300346.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDINMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            8424..9263
                     /locus_tag="KPK24_002683"
     CDS             8424..9263
                     /locus_tag="KPK24_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG2300347.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            9531..11012
                     /locus_tag="KPK24_002684"
     CDS             9531..11012
                     /locus_tag="KPK24_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2300348.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVYDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            11045..14482
                     /locus_tag="KPK24_002685"
     CDS             11045..14482
                     /locus_tag="KPK24_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG2300349.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLITYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVELGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            14500..15246
                     /locus_tag="KPK24_002686"
     CDS             14500..15246
                     /locus_tag="KPK24_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG2300350.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(15195..15503)
                     /locus_tag="KPK24_002687"
     CDS             complement(15195..15503)
                     /locus_tag="KPK24_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG2300351.1"
                     /translation="MNFLKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIEKYKT
                     SYKQLLVNPNIEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         6520 a         1548 c         2614 g         5094 t
ORIGIN      
        1 taagtgctcc aaaaaattta aaattgctta aggaaacttt ggatatagat gaaataactt
       61 ttaatgattg tcttaaattt gatgaaataa ccaaacttga tttatttgat aattacgatt
      121 ttttaagcct aaatacattt gaactaagag atggagaagc tgttatagaa gaggtaaata
      181 tgtatttatc agataatttt atattggttg tagtgaatga agaacacttt ttatttgaat
      241 ttgtaaaaaa tattattttg aagaactctc aacttgaaaa aaatccagtt attaatctat
      301 ttaagataaa ctacttgatt cttagagaag ttataaaaaa tggttttgag tctttggaaa
      361 aagttgaaga gttaattctt caaatagaag atgaaatgat ggacaacatt aataagaatc
      421 atgtatctag aataagtgat gtaagaggat taacaagaat tatagttaag aatactagac
      481 ctcttttgta tattggggat agaatagtta aggaaaatat aagatattta aaatattcaa
      541 atgtaaaaaa atataattta gaaaattttc aaggtataga ttttggaatt gacaaattat
      601 acaatttcgc attgtcaaca cgagagttag cagataaatt attagatatt tattcttcca
      661 gagtaggtga gaaaacaaat aacttaatta caaaattaac cttgttaaca gccatatcag
      721 caccacttac aattataact ggtatatatg gaatgaactt tagatatatg ccagaattaa
      781 actggattta tggatatcca gcaactttat tttttatgct atgtataatt tttgttggaa
      841 ttataatatt taaaataaaa aaattattgt aaattacaga tgatgtttaa ttgattttat
      901 aaaaatacat caattgagaa gctgatttaa gtaaataaaa aaatatttta aattgaaaat
      961 atttatagaa aatattgaca attatatttt ctttgttata attattaaac aagaagaata
     1021 ataaattata aaaatggagg ataatactat gaaaggaata ttaaaagaac taacagcaaa
     1081 actttatagc tttttttact ttatgttctg gggcttttat tttagcgctg gctatttctt
     1141 ttacaaaaat atatgtactt cctttctggt gaggccttat tttagtgatt ttgagcataa
     1201 gtaaatttta taaaaatgat tattttatat atttatataa gatttagtgc ttgatagatt
     1261 attttagaga actcattagt gagttctctt ttttatttta aatataaaat tataatattg
     1321 ggaggtagta aaaatgatag tagtattaaa aatgggagca gataaaaatg aagttaaaaa
     1381 attaattgag gctattggga gagaaggagt agaagttaat cccatagatg gaacagaatt
     1441 aactgtttta ggtttagttg gagatactag taaaatagat gccaaaagaa tagaagcaaa
     1501 taaggtagtt gaaaaagtta tgcatgtggt agagcctttt aaaaaggcca atagaaaatt
     1561 tcatccagaa ccttcaatca taaatgttaa tggaatggaa attggttcaa agaaaattgc
     1621 tatgatagct gggccttgtt ctgttgaaac agaagaccaa atagtatcta tagctaaaga
     1681 tgtgaaaaaa tcaggtgcag gatttttgag aggtggagca ttcaaaccaa gaacatcacc
     1741 atacgctttc caaggattga aatatgatgg attggattta ttaaaaaaag caaaagaaaa
     1801 aacagggctt cctatagtta cagaaataat gtcaacacaa gatatagata tatttgaaga
     1861 aaatgtagat gtaattcaag ttggagctag aaacatgcaa aactttgatt tattaaaaga
     1921 attaggtaaa acaaataaaa caatactttt aaaaagagga ttatctgcta caattgaaga
     1981 gtggctaatg tcagcagagt atataatggc tggaggaaat gaaaatgtag ttctttgtga
     2041 aagaggtata agaacttttg aaacatatac tagaaatact ttggatttaa gtgcaatttt
     2101 agctgtgaag aaacttagtc atttaccagt tatagttgac ccaagtcatg cagcaggtaa
     2161 atcatggatg gttgactctt tatctaaggc agcaattgca gttggagcag atggtctaat
     2221 aatcgaagtt cataatgacc cagcacatgc actttgtgat ggaaaacaat cgattaagcc
     2281 aaatgaatat gatgaattga taagtgaatt aaagactatc gcatcagcag ttggtagaga
     2341 aatataatct ataaaaggat ggttaaacgt ggaagaaaaa ctattagtaa atttaaaaga
     2401 aaatagctac aatatattta ttaaaaaaaa tatactaaag aatataggag aagaaataaa
     2461 aaaaatatat tttggaaata agatttttat tgtaactgat gaaaatatag caaaattata
     2521 cttggataaa gtaaaaaata atttaagtga atctggattt gaaattaaat ctttaatatt
     2581 aaaatctggt gaagaaacaa aatcatttaa tactcttcct aaaatatacg atagtttact
     2641 tgactttaag ttaactagaa aagatttgat tattacttta ggaggagggg taataggtga
     2701 tttaggtgga tttgcagcag caactttttt aagaggtata agttttattc aaattccaac
     2761 atctttatta gcacaagttg atagttctgt aggtggaaaa gttggagttg accttgagag
     2821 aggcaaaaac ttagtgggaa gcttttacca accaaaggct gtgtttatcg acccaaatgt
     2881 attattatca cttccagaaa aattctatag agatggaatg gcagaagtta taaaatatgg
     2941 atgtataaaa gacaaaactt ttttcaataa acttttaagt ttaaaaaata gagaagaagt
     3001 aatgcaacat atagaataca ttatacataa atgttgcttt attaaaaaaa ctgtagttga
     3061 agctgatgaa aaagactttg gagatagaat gcttctaaac tttggccata cattaggtca
     3121 tgctattgag aaatactaca attttacggg ttatacccat ggagaagctg ttgccatagg
     3181 aatgtataag ataactttag aaagtgaaaa gataggaatt acaaaaaaag ggacatcaga
     3241 acttataaaa tcaattttaa tgaattataa tttgccttat gagattgaat taagagataa
     3301 taaatctata gtagatgcta tatctcttga taaaaaaaat ctaggtgata cattaaaaat
     3361 aatactttta aaagatattg gagagagtat tatatataat actacttcta aattttttca
     3421 ggaggtaaga taatgggaag tctaaaaata tatccaagta aattatctgg agatgtaaaa
     3481 ataccaccat caaaaagtat ggcacatagg gcagttattt gttcatctct aagcaatgga
     3541 aaaagtagaa tttctaatat agatttttct gatgatataa tagctactat aagagcaatg
     3601 acatcattag gagctattat agaaaagaaa gaagatattt tagaaatttc gggaattttt
     3661 tctaaagaag gtattttaaa tagagagaat cagttgaatc aacctaaatt gacaatagat
     3721 tgtaatgaat caggctctac attgagattt ttagtaccta tatctttagc atttgatggt
     3781 gtaaaaaggt ttataggtag aggaaatctt ggaaaaagac cacttgatac ttattatgag
     3841 atatttgaca gacagaatat aaaatatagt tacaaagaaa atcagttaga tttgattata
     3901 tcaggtaagc ttaaaccaga tgaatttaga gtaaaaggaa atataagttc tcagtttatc
     3961 acaggtttac tgtttatatt gcctacgcta gagagtgatt caaagataat aattactact
     4021 gagctagaat ctaaaggata tttagattta actctttcaa cgataaaaga ttttggggta
     4081 gaaattataa acaataacta taaagaattt attataaaag gaaatcaaac gtataaagcg
     4141 agagattata aagtagaagg agattattct caaggagcat tttatttatc agcagatgct
     4201 ataggagaag atattagcat attagacctt aaagaagatt ctcttcaagg ggatagtgaa
     4261 gtagttgaaa ttttatctag gatgggaatg gaaatattaa gagaaggcaa caaaataaaa
     4321 ggaataacaa atggtttaaa tggtactttg atagatgcat ctcaatgccc agatataata
     4381 cctgtattat ctgtagtagc atccttaagt atagggagga ctacaataat taatgctggt
     4441 aggcttagaa ttaaagaatg tgatagactt catgcaataa atgttgagtt aaataaactt
     4501 ggagcaaata tagaggagaa agaggatagt ttgataatcg aaggagtaag caagttaaat
     4561 ggtggagtag aggtgtggag tcacaaagac catagaattg ctatgacact tgcaattgct
     4621 tcttgtagat gtgataaacc aataatatta aaagattttg agtgtgtatc aaaatcttat
     4681 ccacattttt ttaaagattt taagatgtta ggaggtcgaa ttgatgagtg gaatatgggg
     4741 aaataattta aaagtatcaa tttttggaga atctcatgga aatgctatag gtataaatat
     4801 agatggtctt ccttcgggga ttgaacttga ccttgataat attgataaag aaatgaagag
     4861 aagagcacct ggaaaaaatt ctatatctac ttcaagaaat gaaagtgata taccagaaat
     4921 tctaagtgga tattttaatg gaagaactac aggaacgcca ttatgtgcaa tcataagaaa
     4981 tagtgatact cgttcaaaag attatggaga gcttaaaaac ttaatgagac caggacatgc
     5041 agatttcaca ggtaatgtga gatattctgg atttaatgac tatagaggtg gaggtcattt
     5101 ttcaggtagg ataacagctc cattagtatt ttgtggggct atatgtaaac aaattttatc
     5161 acaaaaagga atagagatag gagcacacat aaaaaaaatt aaaaatatag aagatatgag
     5221 ttttgattat gtaaatattt caaaacaaca gttatcaaat ttgcaaactt tggaactacc
     5281 tttacttgat ttatcaaagg aagaagccat gaaaaatacg attatagatg caaaaaatca
     5341 aggagattca gttggtggta taattgagtg tgcagtagta ggtattaatg tagggcttgg
     5401 aaatccattt tttgactctg ttgaatctac tttatcacat ttattgtttt ctgttcctgc
     5461 tgtaaaaggt gtagagtttg gtttaggttt tgaacttgca gatatgtatg gctctcaaag
     5521 taatgatgaa atgtattatg aaggaaatca agttaagagc aaaacaaata acaatggagg
     5581 aattatagga gggattacta ctggaatgcc aataattttc aaagtggcaa ttaagccaac
     5641 tccatcaatt agcagacaac aaaacacagt taatataaaa gataaaaagg acgatatttt
     5701 atatattaaa ggcagacatg acccttgtat agttcaaagg gttatacctg taatagaagc
     5761 agtaacagcc attggaattt ttgatttgat gaaaggacga taataatgga ggatttaacg
     5821 agatatagaa atgagattga caatatagat aaagagctta ttcaattatt tgaaaagaga
     5881 atgaatacag ttcttgaaat tgccagacat aaaatgaaaa ataacactac tatacttcaa
     5941 aaagatagag aagaaaaagt actaagtaga gccgtagata atttagataa taaagcatat
     6001 tcacaagaaa cggtacagtt ttttaattct ataatggaaa ttagtagagg attacaaaaa
     6061 agatttatag ataatgatgg tgagcagaaa atcgagttta gtttagattc atcaaataag
     6121 aattttgatt tgtcaaatgt aaataaatat aagtctttaa aagatgaatt aaataaaaaa
     6181 aatatcctag taggctttcc tggaaaatca ggttcattta cagaagaagc actaaataaa
     6241 ttcttcaaca aaaaaatatc aaaaaaacaa tttaaagaat ttgaagatgt tttcatagca
     6301 cttaaaaata aagaaataaa ctatggaata atccccatag aaaattcatc tacaggagct
     6361 atctctgaaa cttatgacct tttgagaaag tacggttttt atatagttgg agaagaatgt
     6421 ataaaaatag accaaaattt aatagggata aaagacacaa agcttgaaga tataaaagaa
     6481 atatattctc atccacaagg tataggacaa tgtagtgaat ttttaaaaca aaatagtaca
     6541 tggaaattga taccctttaa taacacagca acaagtgcag aacttgtaag aaaacttcaa
     6601 gataaaacaa aagcagcaat tgcaagcaaa aaagctgcaa gtatttatgg attagaaata
     6661 ataagtcctt gtataaatga tatagcaaac aactatacaa gatttgtagt aatcagtaat
     6721 caaatacaca ttgaagaaga atcaaacaaa atgagtgtag tattttctgt tgaacatgaa
     6781 gcaggaaaac tttataaagt acttggatac tttgctaaaa ataatataaa tatgacaaaa
     6841 atagaatcaa gacctatgaa aaatgcctca tggagatatt tcttttatat agattttgag
     6901 tgttctatat acaatgctaa agtgtacgat ttattagaat tgatagaaca taatacagca
     6961 tattttaaat ttatgggagt atataaaaat aaaactgaat agataagata aatttttatt
     7021 taatttataa ttattaggag gtgtaagtat gaattttttc ggtctagtag gtgaaaaact
     7081 ctctcacagt gtttcacctc aaatccacaa gagagtattc gagatattga atattgaaag
     7141 tgcttataaa aactttgaaa tttcaaaaga agatatatct aagctagacg gtgctataaa
     7201 attattagga atacaagggg taaatgttac tgtaccatat aaggagcgta ttatgaaata
     7261 tcttgatttt atatcaccag aggcaaaacg tataggagca gttaatacaa ttttactcag
     7321 agaaaatatg ttatatggtt acaatacaga ttactttggt cttgatagta tgtttaaaat
     7381 ggctaatatt gatgttcaag gaaaagtagc agttatatta ggaactggag gtgcttctaa
     7441 agctgctctt acatatttta ttgattctgg tatagaaaag ctttatgttt caactagaaa
     7501 aaaggatgat aaaaaattat taaatagtaa agctatatta atagattatg aagaacttaa
     7561 acatataaaa ggagatataa tattaaatgc tacaccagta ggtatgtacc ctaatgtagg
     7621 aatttcacca gtgtcaaaat ctattataca aaattttgat atattaatag atttaattta
     7681 caatcctgga gaaacagagt ttttaagaat tggtaatagt atgggtaaaa aaacttgtga
     7741 tggattatat atgctggtgg ggcaagcaat aaaatctcag gaaatttggc aagatactaa
     7801 aatagataat agtattttag atgttatata taatgaacta aaattagaat ttttgtaagg
     7861 gagattgtta tgataaataa aacaaaagaa aaattgatat taataggtat gccaggtagt
     7921 ggaaaaacta caattggtaa acttttggcc aaagaatata attgtagttt ctgtgatatg
     7981 gatgattata taattcaaat atctcaaaaa agtatagcag agttgttttc agaaggagaa
     8041 gatatcttta ggaactatga gactcaagca tgtagagaac tttctataag tgataaaaca
     8101 gtgatatcta caggtggagg tgtaataaaa aaggatatta atatggaaat actaaaagaa
     8161 acaggtataa taatttttat tgatagacct atacaaaaga ttttggaaga tataaatatt
     8221 aattcaagac cattattaaa aaacggaaaa gatagattgt ataatcttta caatgagaga
     8281 ataaatcttt ataaaaaatt ttcagatata gagattttaa atgataaatc attaaataat
     8341 gctgtatata atattacaaa tgcagtcagt gaaaatttta aatttgactt taaagaaaaa
     8401 taatctaggg ggaaataaaa ataatgaata ttgttattgt aggactagga gttataggag
     8461 gctcttttgc taaagcatta aaaaaagctg gatatgaaaa tgtatttgga gtagatgtag
     8521 atttagaaac tttaaaaaaa gcagaaaaag caaaaatcat aaaaaaagga tgtactacag
     8581 gtaaagagtt atttaaaaaa gctgatttaa ttatattatc tatatatcct agattagttg
     8641 tagattttct aaataataat aaaaactttt ttaaaaaagg gactataatt actgatacta
     8701 caggaataaa agaaacactt atcaatgatg tgttgcaaat tattccagat gatatagatt
     8761 ttatatttgg acatccaatg gcaggtaggg aaaaaaaggg tattgatttt gcaagtgaac
     8821 aagtttttaa tggcgcaaat tatataatta ctccaactgg cagaaacaac ataaaaaatt
     8881 tagagttagt tgaaaattta attttagaga ttggatttaa aagagtaaaa aagttaactt
     8941 cacaaaaaca tgatgagatt atagctttta cttctcagtt acctcatgtt atggcagttg
     9001 ctcttataaa tagtgatgaa gaaggtagag atacgggtaa atttattgga gatagttata
     9061 gagatttaac tagaattgca aatatgaatg aagacttatg gagtgaactt tttttaggaa
     9121 atagagataa tttactaaaa gttatagaaa actttgaatc agaagtaaat ttagtaaaag
     9181 aagctatatt taacaatgat aaaaataaat taatagagta ttttaaaaaa tcatcaataa
     9241 gaagagaagc tttagaaaaa taaatattaa tatttaagct atatatagat gggtataaat
     9301 ataagcgata gtgtatttaa ttgtattaat aaatatggat tcaatataat taaatatgct
     9361 attgatatat ttgtaccctt aactattgtt atcttttaga tgataatgat atttatttaa
     9421 atactcttta ttaacaattt ataaaactta attgtaaaaa atacttatta atagtaaaat
     9481 aaagtgaatc aattatatta taattgctaa agagagtgag gtgtagctat ttgaaaaaaa
     9541 atagattaat agcaaagaat atatcaatag catttatagt actattcttt tttagtgtat
     9601 ttacattctt ttatgtaggt aatattaata gagtgttaga atatgaaaca aatgacataa
     9661 taacagttac aattgctgga tggattatac tttcattttt atttttaggt attataattt
     9721 acatattata ttcaaaagca aatagtcaaa aaactataga aaaagtagca tatacggatt
     9781 ttgtaacagg ttattccaac tggagaaagt ttgaattaga tgttacaaat ttattaaaga
     9841 aaacatcaca aaataataaa tatgcaatgg tcatattcga tattgataaa tttaaagcaa
     9901 ttaatgatat atatggccat aaaaagggaa atttaatatt aaaagatatt gcagatactt
     9961 taaatgaatt gactgatatc aatgaaactt ttgctcgtgt aagtgctgat aatttcaata
    10021 tattattaac atataacaaa aaagaggata taataaatat tatcaaaaag attatggcaa
    10081 ataatgaact tgtaaactta tcttttggaa tttatgaaat caaagataag gatttatctg
    10141 taagtgtata tagtgataga gcttcacttg caaaatcatc aataaaaaat aatagtgatg
    10201 taaattttgc attttttaat gacaaattaa gagagaaatt attatttgaa gataagatag
    10261 aaaaagaaat ggaatatgct cttgaaagtg ggcaattcgt aatgtattta caaccaaaat
    10321 acaatattaa attagataaa ttttgtggaa gtgaagccct agtcagatgg caatatacag
    10381 aaaaagaggt catatatcca ggtgatttta ttcctatatt tgaaaaaaat ggatttatta
    10441 gaaagattga tatgtacata ttagaacaag cttgtaaaga aatccggtct ttatttgata
    10501 aaggaatttc acctttacct atatctgtaa acttctcaag agttgatttt tttaaaaaag
    10561 attttattga aaacatagta aatatatgtg atagatataa aataccatat agtttaatag
    10621 aaattgaaat tactgaatct agtatgtttg gagatactga tactctattt aatgttagta
    10681 gaaacctaca ggatattggc tttatagttg caatggatga ttttggctct ggatattctt
    10741 ctgtaaatat gcttaaaaat ataccattaa atgtaataaa attagataga ggattttttg
    10801 tttatgataa agatgttgat aagagtcaaa ttgttataaa aagcatagtt tctctcatta
    10861 agcaattagg aataagggtt gttgctgaag gaattgaaac aagaagtcaa atagaaatgt
    10921 taaaaaaagc aaattgtgat attgttcaag gatattattt ttcaaaacca ttaccaatta
    10981 aagaatttga aaaattagtc tataaaatat agtcagcgag tcttaaatgg gaggtggtga
    11041 ttaagtgata agagcaaaat cgttaaaaaa tgtactgagt attgttttaa tatttttatt
    11101 tacaataatt tctttttctg attttgcatt taaaactaat gtccaggaag aagaaaatag
    11161 taaaattaat ttaagagaaa tatccctaca aagttccaat gctataaaaa taaaattaga
    11221 tagttctcta tcctttatta aaagtgtggc aaatatggtt tcttatttta atactataga
    11281 taactcaaaa atagttccat tattagctaa tatatcaaaa gaatctgact ttcaaagaat
    11341 ctctattgta tctcctgatg gaataagtca taccagtgat ggcaaaatta tcaatgtgtc
    11401 aaagtatcag tattttaaga aagctattaa aggagaaagt aatatatctg aagttgtgaa
    11461 atctccaatt gatggaaaag atacttttgt tatagctact cctgtattta ataagaataa
    11521 caaaataatt ggtgttttat acggttctta ctacaatgat aaattagctg aatttataga
    11581 tataacaagt tttaatagtg aagggtacat agatatattt gaatctagtg gtcaatttgt
    11641 tttaaaatct tcacatacaa acaaactgat taagtcaagt agtaacatat atgatatatt
    11701 taaaaaagct tcttttgatg atggttatag ctataaaaaa atatatgaag atacaaaaaa
    11761 taacaagagt ggctttacct catatgaata caaaggagaa aatcgttatg ctacttatac
    11821 acccattggt gtaaatgact ggtatgtaat ttctgtgata ccacaatata ctgtaagaag
    11881 aaacattgat aaaataaatc aattagcatt ttctcttatt ttcaaggtat tcttattgtt
    11941 tattatgctt ataacatata ttatttataa aaacaataaa tctcagaaag atatactaaa
    12001 agctaacaaa gaaatctcat ctattacaaa tagcattcct ggaggagtac aaaagtgtac
    12061 cctgggtgaa acatgcaata ttaatttttt aagtgatgga tttatagagt tgtttggtta
    12121 tacaagagaa gaaataaaaa cattgtttaa taatgatttt tatagtttaa tacataaaga
    12181 tgatgttgaa agaataaaaa aagaaatttt taaacaactt tacaaaaata aaactgttga
    12241 attagaatat aggattataa caaaaaatgg taatacagta tgggttttag ataaagggga
    12301 gcttataaca gaacctgatg gaaaagaata tttttgttgt gtaactgtag atattactag
    12361 aacaaaacaa ttagaacaag agatggaaca aaaaaatcta gaacttgaaa tgatatcaga
    12421 aaatgtttta ggtggtataa ttataacaga atttaatgat gattttacta taaaatacgc
    12481 taactctggt tatctttcaa tgattggata tacacgtgaa caattagaaa atgaacttaa
    12541 atacaaagct tgtaatcttg ttttttatga agatagaaag aaggctcttg aaagtataca
    12601 acatcaatta aacacaaaag gaatagctgt atatcaacat agaataagaa gaagagatgg
    12661 taatattatt tgggtttcta taaaaggaag gcttattgtt gatgatgatg gtattgaaaa
    12721 aggtatttgg attctaaatg atataacaga tttaaaaaat gcagaacaaa aacttaaaat
    12781 aaatgaagaa agatttagaa ttgcattaaa gcaaacatca aacactatat ttgagtatga
    12841 tataaaaaca aagtcacttt taaatactag taaagagctt gataaatata atatttataa
    12901 aattactaaa aatgttccat attctctagt agaattagga gttattcata aagattttag
    12961 tgatgatttt ataaatatgt atgaaaatgt aataaatgga gcaaaaaaag caagttgtgt
    13021 agttaagatg aaacttccag atgaaaatta tatatggaat aaagttacct tgactacgat
    13081 ttttgatgaa gatggtaatc ctgtaagagc aattggtata caggaagata taagcaaaca
    13141 aaaggaagca gagataagat acaatcaaga aggtaagtat cattcagcta tattgtcgga
    13201 agcaatgata acttatgaaa taaatgtatc aaaggatact tttatcaaag gaaatgaaag
    13261 ttggcaaaaa ccatttggaa ttaagcctga caataactat tctcaaatgg taaaaactat
    13321 agcaaatagt attgtacata aagacaataa agaaacattt ttaaatacat ttttatgtga
    13381 aaacttattg gatgcatata aaaataatgt gactgaaata aaattagaat atcgaagaat
    13441 gaatcaagaa aataaaatga tttgggtcat ttgtacaatg cacttaataa aagattctat
    13501 tacgaacgat ataaaagcac ttgcttatgt taaagatatt gacaagcaaa aaaatgaaga
    13561 aattagatta aaattaaaag ctgaaagaga tactcttaca ggattatata ataaaggtac
    13621 tacagaaaaa ttgattaatg atttcttgaa ctctgaggaa gcaagcttag gaatgcatgc
    13681 atttttaatt gttgatatag ataattttaa atctgtgaat gataatttag gacatgtatt
    13741 tggagataaa gtattatttg aagtttctaa aaacctaaaa cctatattta gaaaagatga
    13801 tatagtaggt agaattggtg gagatgaatt tatagtattc ttaaaaaata ttcagtcttt
    13861 tgagaatatc aaaaagaaag cagaaaaaat atgcgatata tttagaaatt cttatacaga
    13921 aaataaaaag acatataaaa tatctggaac agttggaatt tcatttgtac cagaacatgg
    13981 aactgtattt gaagaacttt ataaaaaagc agacatagca ctttattatg caaagagcaa
    14041 aggaaaagat aagtatgcac tttataatga ttgtatctac caaaatgaac ttgaaaataa
    14101 tataagtgat aataatataa agttagcaga taaatgtgag aaatccaaac cagtacttaa
    14161 agaaattctt gattctattg ataattatat ctatatagtg aatccaaata cttatgaact
    14221 tctttttgtc aataataagg tgtctcgttt ggatttagag ataaaattag gagaaacctg
    14281 ttataaatca ctattaaaat ctgatagacc atgtaataat tgccctctta acggattaga
    14341 agaaaataag cattcaaaat tcaaaataga gacaaataca tttggtaaaa atatcagtat
    14401 agcaagttgg attaattgca tagatggaga atacaactgt cttatagagt gtatagattc
    14461 ttctttatac aaaaataaat aaaaaaggac tatgaagata tgttaaatag tataactttg
    14521 atacttggta taatatttac aatttactat ttttatctca aaatagtttt tggaggaatt
    14581 tcatttagtg agattttctt agttgtagga attgtactaa ttatctatca aatatttaag
    14641 aaaaaaatta aagaaaagaa ggttttttat agggtgttaa aaataataat cagcattttt
    14701 ttaatagtat ttgtagttac tgagagtcta attatttttt atcccaaaaa tagtttagaa
    14761 tcaaaaagtg attatctctt aattttagga gcttctgtaa aaaagactac accaagtaca
    14821 accctgaagg gtaggttaga cactgcattg aaatacctaa aaattaatga taattgttat
    14881 gtggtagtat ctggtggtaa aggaagtgga gaaaaaataa ctgaggcaaa agctatgaaa
    14941 gattatctta taaaaaatgg tatagataaa aataggataa tagaagagga taaatccacg
    15001 aatacttatg agaattttaa atattcaaaa ttgaagatag aagaacatag tcaaagaaaa
    15061 ctaagcgatt taaaagtaaa aatagtaaca acagattttc atgttctcag aagtaaaata
    15121 ttagcatata gaaatggata taaaaatacg tctttttatg ctagtaaatc aaaactttca
    15181 tttgtaccaa cttactatac aagagaattt tttgcattat ggaaaacaat catttttgat
    15241 agataataaa taaaaagact agatataaac tcatatctag tcttttctat tcccttaatt
    15301 ctaatccatt ttccaacttt acttgttgta gaaatttcaa tattgggatt tacaagtaat
    15361 tgcttataag aagttttata tttttcgatt ccaaaatata attttccttc ataatccatt
    15421 ataaatccaa atggtcttac tcttggttta ttatcttcaa ctgtagcaaa atagaatgtt
    15481 gagttttctt ttaaaaaatt cattattttt tcatgattaa atcctctttg tatcttatac
    15541 taatataatg aatataaata aaatattcat tatacttaat ataaatcgat ataaaaacaa
    15601 ttacaatatt atattgatta ttcgaaatta tatataaaat tcagaaaaaa ttttagttta
    15661 cataacaaaa ataaagtcaa ctaaaattat tgattttaga ttaaagtacc ggattaagca
    15721 tttaagtaga ttttaagctt gatttgattg gaaaggtaca aactatcacc gaaaaa
//
LOCUS       DAEMGG010000094        15416 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEMGG010000094 DAEMGG010000000
VERSION     DAEMGG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.094"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..139
                     /gene="rrf"
                     /locus_tag="KPK24_002688"
     rRNA            23..139
                     /gene="rrf"
                     /locus_tag="KPK24_002688"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            147..221
                     /locus_tag="KPK24_002689"
     tRNA            147..221
                     /locus_tag="KPK24_002689"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:179..181,aa:Asn,seq:gtt)
     gene            227..312
                     /locus_tag="KPK24_002690"
     tRNA            227..312
                     /locus_tag="KPK24_002690"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:261..263,aa:Leu,seq:taa)
     gene            328..403
                     /locus_tag="KPK24_002691"
     tRNA            328..403
                     /locus_tag="KPK24_002691"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:361..363,aa:Met,seq:cat)
     gene            411..485
                     /locus_tag="KPK24_002692"
     tRNA            411..485
                     /locus_tag="KPK24_002692"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Glu,seq:ttc)
     gene            500..573
                     /locus_tag="KPK24_002693"
     tRNA            500..573
                     /locus_tag="KPK24_002693"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:532..534,aa:Gly,seq:tcc)
     gene            579..654
                     /locus_tag="KPK24_002694"
     tRNA            579..654
                     /locus_tag="KPK24_002694"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Val,seq:tac)
     gene            660..736
                     /locus_tag="KPK24_002695"
     tRNA            660..736
                     /locus_tag="KPK24_002695"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:694..696,aa:Asp,seq:gtc)
     gene            747..821
                     /locus_tag="KPK24_002696"
     tRNA            747..821
                     /locus_tag="KPK24_002696"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Gly,seq:gcc)
     gene            831..904
                     /locus_tag="KPK24_002697"
     tRNA            831..904
                     /locus_tag="KPK24_002697"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:865..867,aa:Arg,seq:tct)
     gene            1888..2727
                     /locus_tag="KPK24_002698"
     CDS             1888..2727
                     /locus_tag="KPK24_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG2300352.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETISFFKGG
                     IFK"
     gene            2724..3905
                     /locus_tag="KPK24_002699"
     CDS             2724..3905
                     /locus_tag="KPK24_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300353.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            4209..5111
                     /gene="ptb"
                     /locus_tag="KPK24_002700"
     CDS             4209..5111
                     /gene="ptb"
                     /locus_tag="KPK24_002700"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG2300354.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            5133..6212
                     /gene="buk"
                     /locus_tag="KPK24_002701"
     CDS             5133..6212
                     /gene="buk"
                     /locus_tag="KPK24_002701"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG2300355.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            6202..6879
                     /locus_tag="KPK24_002702"
     CDS             6202..6879
                     /locus_tag="KPK24_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2300356.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6922..7137
                     /locus_tag="KPK24_002703"
     CDS             6922..7137
                     /locus_tag="KPK24_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2300357.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            7166..8245
                     /locus_tag="KPK24_002704"
     CDS             7166..8245
                     /locus_tag="KPK24_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG2300358.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            8245..8997
                     /locus_tag="KPK24_002705"
     CDS             8245..8997
                     /locus_tag="KPK24_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG2300359.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            8998..9555
                     /locus_tag="KPK24_002706"
     CDS             8998..9555
                     /locus_tag="KPK24_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG2300360.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            9725..11071
                     /locus_tag="KPK24_002707"
     CDS             9725..11071
                     /locus_tag="KPK24_002707"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG2300361.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            11338..13170
                     /gene="glmS"
                     /locus_tag="KPK24_002708"
     CDS             11338..13170
                     /gene="glmS"
                     /locus_tag="KPK24_002708"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG2300362.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISREDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            13622..14917
                     /locus_tag="KPK24_002709"
     CDS             13622..14917
                     /locus_tag="KPK24_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG2300363.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
BASE COUNT         5996 a         1799 c         3058 g         4563 t
ORIGIN      
        1 gaaatattaa gcataaagat tatgtggtta caatagcaga gaggatacac ctgttcccat
       61 tccgaacaca gaagttaagc tctctagcgc tgatggtact tggtgggaaa ctgcctggga
      121 gagtaggacg tagccacgtt aaatgttcca aggtagctca acggtggagc aaccggctgt
      181 taaccggtag gctgtgggtt cgagccccac ccttggagcc aaaaaagccg aagtggcgga
      241 actggcagac gcacaggact taaaatcctg cgggacttac ctctcgtacc ggttcgattc
      301 cggtcttcgg cacttataaa acaatatcgc ggggtggagc agttggcagc tcgtcgggct
      361 cataacccga aggtcgtagg ttcaagtcct gcctccgcaa ccattaaaac ggctcgttgg
      421 tcaagaggtt aagacaccgc cctttcacgg cggtaacagg ggttcaattc ccctacgagt
      481 caccaattca attaaatatg cgggtgtagc tcaatggtag agttctggcc ttccaagcca
      541 gctgtgaggg ttcgatcccc ttcacccgct ccaaaaatgg gaccatagct cagctgggag
      601 agcacctgcc ttacaagcag ggggtcacag gttcgagccc tgttggtccc accattcgcg
      661 gcctggtagt tcagttggtt agaatgccag cctgtcacgc tggaggtcga gggttcgagc
      721 cccttccagg tcgccaaatt aaataagcgg gaatagttca gtggtagagc gcaaccttgc
      781 caaggttgaa gtcgcgagtt cgaatctcgt ttcccgctcc ataataactt gggtgcatag
      841 ctcagctgga tagagcaacg cccttctaag gcgtgtgtcc ggggttcgaa tccctgtgcg
      901 ctcattaaga aaagtttact ataaattata gtaaactttt atattgttta aaaagacaca
      961 ggaaacatgt atattacctg tgtcttttta tttataaact ttaaactatg taaaaattac
     1021 agtatattct aatagagtta cagtatattc attaatattg taaacatacg gagggtaatg
     1081 tataatagtc tacaagatgt ttatgaaggc atcagttaaa tataataaat ataactaatt
     1141 agtaagagtt atatacatta tattgtatac tttatggtat ttatatattg aaataactta
     1201 tgaaattatt tataaagaga ttcatgagca tatttttgtc taaacaataa gtataagaaa
     1261 ttataaaaaa ttagagacta tagtgaattt acaatttaat gtaatattta taagttatac
     1321 taaataaatt attatactgt gaaatggata tataacttta ggtattgtgt attattttca
     1381 ctgtaggaga ttatatctag ttatacataa taagttttaa tgatagttta tagtaagata
     1441 ttaaaataaa tttatctaat ctagatatga aataagttat tactttaata taaaaatgaa
     1501 tctaatggaa taatattgat gataagtaat atttagattt tgtatttgtt tatagttaat
     1561 aagtggatta tagaatttat aataattaat atatattaaa caaagatata ttaagatatt
     1621 ttagaacata aaactgaatt aaggcgcgag tactattttg tattcgcgcc ttgatggttt
     1681 gagttatttg agtaaaaact aactttaata agaaatatgt atttattaag ttgatatttt
     1741 taatgtgttt agagattatt taacaatctc caaatataaa actagaatta tttacataaa
     1801 agttaaatag tttgtataga gttagattta tatagtataa tacttagtgt aaataattaa
     1861 gaagttatca aacaggagga taaaactgtg ttagatataa attctttttt aaatggattt
     1921 tttaatataa tattaagaat gagtatatat gatttaattg acatatctat agtagcgtat
     1981 attttttaca aaatatttat gtttataaaa gatacaagag cagaacaagt gtttaaaggt
     2041 ataatctttc ttttattagc aactcaactt agtaacacct ttaaattgca taccgtttat
     2101 tggatatctc ttaaagcttt agattatgga gttatagctg ctcttataat ttttcaacca
     2161 gaatttagag caggacttga acatatagga agagcaaagt ttaatttatt tggaaagaat
     2221 gtaaatactt ctgaagaaac tctcaataga aatatagaag aaattgtaga agctttatat
     2281 tcactttcta gacaaaaaat aggagctctt atcataatgg agagagaaac tagaataagt
     2341 gatataataa atacaggaac tattattgat gctgaaattt ctaggcaact attaataaat
     2401 atatttatac caaatacacc attacacgat ggagctgttg taattagaga ttctaaggta
     2461 aaagcagctg catgtttttt gccattgaca gaaagtaagg atttaagtaa agatttgggc
     2521 actagacata gagcaggaat aggggtaagt gaggtgtctg actgtataac tctgattgta
     2581 tcagaggaaa caggtggcgt atcaattgct aaagcaggta agttatatag agatatttct
     2641 agagaaagaa tgatgaatat attacgtagt aatttaaaaa caaatacaga aacaataagt
     2701 tttttcaaag gtggtatatt taaatgataa atagactaaa aaataatacc aaaataaaaa
     2761 taatatcatt tttaagtgca attgcattat ggatgtatgt tatggctgta gttgaccctg
     2821 aagaaactaa agggtttgaa gatatacctg tatctataag taatatgagt gatttaaagg
     2881 ataaaaatct tgttatatat ccagacgaaa aattaacagc agatgtatat ataaaaggta
     2941 gtttatcaaa ggtacaaagt gttaaaaaag aagatataca tgtatatgga accattgaag
     3001 acccaataca aggacagaag gaagtatatt taaaagcaaa tattcctcaa ggtgttactc
     3061 tagaatttaa gcatgatgtt ttagtagtaa atcttgaaaa aaacataaaa gaaaaaagag
     3121 atataaaggt agaagtaagt ggaaattcaa aaagaaatat aaataaaatt gaacttagta
     3181 aggaatttgt cgatgtatca ggtccaagaa ctctagttaa agaagtcaaa agtgtaagag
     3241 ctaatctaga tgttggtaat gagacagatg atttttctaa aaaattgcaa ttaataccat
     3301 taaatagtga tggaactaag gttgaaggag taacattaag ttcatccttt gtgactgcaa
     3361 agatagaact attgaaggaa aaaattgtcc caataagagt aagtctaaat gataataaag
     3421 ataatgatgt actgaaaaat tataaattaa gtaaggaaca agttacaata aaaggaaaga
     3481 aagaagatat agacaaaata agttatatta agacaaaacc tattgactta gctgatttgg
     3541 ctaatggtga agaaaaagaa gttagcttag aaattccaga gggtataact cttgaggata
     3601 gctctataac aattaaatta aatgatacaa aacaattgag tgcagaattt ttatataatg
     3661 cgggagatat tgaattaaga aatatcccaa caaatatgga taatccaaat gtaatttctg
     3721 atgtagatat aaaagttgtt gtagagtcaa cggaggattt gagtaagtta aataaaaaag
     3781 atatttcatt atatattgat ttatcacaag gtcctgagga agatagtaaa tataaaataa
     3841 aatatgaaac aacatatcaa tttaaaaaaa taaatataga acctaatatt gttgaagtgg
     3901 aatgaacaaa tatttcgaat atttagttgt tacaagatgc gacaaaaaaa cctataaaat
     3961 taagtttgtt aattttatgt ttttgttgca tcttgtttat tttagttata ttataaaata
     4021 aggacaatga gatatttgtt aaatctaaaa agcgctagat atatgaaaaa agactgataa
     4081 atagatttaa ataaatattt tgaattaaat atttcaatta tctctaggaa aacgttgctg
     4141 aacgagttat aatttaattg agatataaaa agttctattt caaaaaattc aagatgaggt
     4201 ggaaatgcat gagaagtttt gaagaagtaa ttaagtttgc aaaagaaaga ggacctaaaa
     4261 ctatatcagt agcatgttgc caagataaag aagttttaat ggcagttgaa atggctagaa
     4321 aagaaaaaat agcaaatgcc attttagtag gagatataga aaagactaaa gaaattgcaa
     4381 aaagcataga catggatatc gaaaattatg aactgataga tataaaagat ttagcagaag
     4441 catctctaaa atctgttgaa ttagtttcac aaggaaaagc cgacatggta atgaaaggct
     4501 tagtagacac atcaataata ctaaaagcag ttttaaataa agaagtaggt cttagaactg
     4561 gaaatgtatt aagtcacgta gcagtatttg atgtagaggg atatgataga ttatttttcg
     4621 taactgacgc agctatgaac ttagctcctg atacaaatac taaaaagcaa atcatagaaa
     4681 atgcttgcac agtagcacat tcattagata taagtgaacc aaaagttgct gcaatatgcg
     4741 caaaagaaaa agtaaatcca aaaatgaaag atacagttga agctaaagaa ctagaagaaa
     4801 tgtatgaaag aggagaaatc aaaggttgta tggttggtgg accttttgca attgataatg
     4861 cagtatcttt agaagcagct aaacataaag gtataaatca tcctgtagca ggaagagctg
     4921 atatattatt agccccagat attgaaggtg gtaacatatt atataaagct ttggtattct
     4981 tctcaaaatc aaaaaatgca ggagttatag ttggggctaa agcaccaata atattaactt
     5041 ctagagcaga cagtgaagaa actaaactaa actcaatagc tttaggtgtt ttaatggcag
     5101 caaaggcata attttaaggg ggaaataggc aaatgagcaa aatatttaaa atcttaacaa
     5161 taaatcctgg ttcgacatca actaaaatag ctgtatttga taatgaggat ttagtatttg
     5221 aaaaaacttt aagacattct tcagaagaaa taggaaaata tgagaaggtg tctgaccaat
     5281 ttgaatttcg taaacaagta atagaagaag ctctaaaaga aggtggagta aaaacatctg
     5341 aattagatgc tgtagtaggt agaggaggac ttcttaaacc tataaaaggt ggtacttatt
     5401 cagtaagtgc tgctatgatt gaagatttaa aagtgggagt tttaggagaa cacgcttcaa
     5461 acctaggtgg aataatagca aaacaaatag gtgaagaagt aaatgttcct tcatacatag
     5521 tagaccctgt tgttgtagat gaattagaag atgttgctag aatttctggt atgcctgaaa
     5581 taagtagagc aagtgtagta catgctttaa atcaaaaggc aatagcaaga agatatgcta
     5641 gagaaataaa caagaaatat gaagatataa atcttatagt tgcacacatg ggtggaggag
     5701 tttctgttgg agctcataaa aatggtaaaa tagtagatgt tgcaaacgca ttagatggag
     5761 aaggaccttt ctctccagaa agaagtggtg gactaccagt aggtgcatta gtaaaaatgt
     5821 gctttagtgg aaaatatact caagatgaaa ttaaaaagaa aataaaaggt aatggcggat
     5881 tagttgcata cttaaacact aatgatgcta gagaagttga agaaagaatt gaagctggtg
     5941 atgaaaaagc taaattagta tatgaagcta tggcatatca aatctctaaa gaaataggag
     6001 ctagtgctgc agttcttaag ggagatgtaa aagcaatatt attaactggt ggaatcgcat
     6061 attcaaaaat gtttacagaa atgattgcag atagagttaa atttatagca gatgtaaaag
     6121 tttatccagg tgaagatgaa atgattgcat tagctcaagg tggacttaga gttttaactg
     6181 gtgaagaaga ggctcaagtt tatgataact aatgataaaa gaataagaat cataactggt
     6241 cactatggaa gtggtaaaag tgaatttgcg atgaactatg ttgttaagtt aagagatatg
     6301 gtttctggca aagttgctat agccgattta gatgtggtaa atgtatactt tagaactaga
     6361 gaaaagaaag agttaatgaa atccttaggt atacaaccaa tagatagctc aattaatgct
     6421 ccaacattag acttaccagc agtatcagca gaggtaatgt ctcctatggt agaccattca
     6481 tacaattctg ttatagattt aggtggagat aatgtaggtg ctagggttat aggaagattt
     6541 agtcatttgt taaaagaagg cgattatgat atgctttttg taataaatgc aaatagggag
     6601 aaaactcaaa cttctgaaga agttatccaa tatataaaag aaatagaaaa atcttctaaa
     6661 ttaaaggtaa caggtctaat taataatact catcttatta ggtttactac tattgatgat
     6721 gttttaagag gtcagaaagt tgcaaaagaa gtttctgaaa aatgtaatat accgataaga
     6781 tatgttgcat gtttagaaaa tcttgttgaa cagttaccaa aagatttaga aggagaaatt
     6841 tttcctataa aattatatat gagagaagac tggatgtagt ttgagctcaa atatatagaa
     6901 agtttaagga ggcttaggta aatggctaaa ggaaaagtat cttttaatca agaacgttgt
     6961 aaaggttgtg gattgtgtgt tgaagcatgt ccagtaaaaa tcatacaatt agactcaaat
     7021 gtaataaaca aaaaagggta taacccagca actgtttttg aaatggaaaa atgcatagga
     7081 tgtgcaagtt gtgcaactat gtgtccagat gtagttataa cagtagaaag agattaattt
     7141 aattaatatt taagggggaa ttttaatggc taagatactt atgaaaggta acgaagcatt
     7201 tggaaaagca gctatagaag ctggttgcaa atatttcttc ggttacccaa taactccaca
     7261 aagtgaatta ccagaatatt tatcaagaga gttacctaaa ataggtggag cgttcgttca
     7321 agctgaatca gaagtttctg cgataaacat ggtttatggt ggagcaggtg caggagcaag
     7381 agttatgact tcttcatctt caccaggagt tgctttaaag caagaaggta taacatatgc
     7441 agtaggagca gaagttcctt gtgttgtact taatgtaatg agaggtggtc caggacttgg
     7501 aagtatacaa ccttcacaag ctgactactt tatgtctaca agaggtggag gaaatggaga
     7561 ctacagaact ccagtattcg caccagctac agttcaagaa gctgttgata tgataatgga
     7621 agcttttgac gttgcagatt attacagatc accagttatg gtagttgcag atggtatgat
     7681 aggtcaaatg atggagccag ttgaatttag agctcctgag aagaaaagag aattgcctcc
     7741 taaagattgg gcaactgttg gaactaaagg aaaaagaaaa cctaatgtaa taaactctct
     7801 atacttagag ccagaagtac tagaagacca ttgttggcat ttacaagaaa aatttgatgc
     7861 aatggaaaag aatgaagtac aatacgaaat gtacaaaaca gaagatgctg aatttgtatt
     7921 tgcagcttat ggaactactt caagagtagt aaaaagcgca attgatatat taagagaaga
     7981 aggaataaaa gcaggtctta taagacctaa agtattatgg ccattcccat tcgaagcatt
     8041 caaccaaatt cctaatgcta gaaacatatt gactgttgaa atgagtatgg gacaaatggt
     8101 ggaagatgtt aaaatggcag tagaaggtaa attaccagtt tacttccatg gaagaccagg
     8161 aggaatgact ccaacaccag cagaaatagt tgaaaaagct aaaaaaatta ttgccggaga
     8221 attggttgca ggaggtgcta gataatggct gttgtattta agaaaactga aggattacaa
     8281 gatactcaaa ctcactactg tccaggatgt acacatggta taatccatag attagttggt
     8341 gaagtattag aagaactagg agtattaggt gatgctgtag gtgttgttcc agttggatgt
     8401 tcagttttag gatataaata ttttaactgt gacactcaag aagctgctca tggtagagcg
     8461 ccagctgctg ctacaggaat aaaaagagtt catcctgaaa atacagtatt cacttatcaa
     8521 ggtgatggag acttagcttc aattggtact gctgaaatag ttcatgcagc tgctagagga
     8581 gaaaaaataa ctactatatt tgtaaacaat acaacttatg gtatgactgg tggacaaatg
     8641 gctccaacta cattagttgg acaaagagct actactgctc aatcaggaag aaatgctgaa
     8701 actcaaggtt atccaataag agtaagtgaa atgttagcta cattaacagg agctgtattt
     8761 gtagaaagag tagcagttga tactccagct catgttagac aagctaaaaa agctataaag
     8821 aaagcattcc aagttcaaca agcaggatta ggatttggaa tagtagaagt attatctact
     8881 tgtcctacaa actggggact agctccaaat gatgctttac aatggttaag agacaacatg
     8941 ataccatact atccattagg aaatttcaaa aatgttgaag ttgaggaggt gaagtaaatg
     9001 tctacagcta gagtaatatg tgcaggcttc ggaggccaag gtgttatgtc tatgggacaa
     9061 ttacttactt atgcaggaat gctagaagga aaagaagttt cttggttacc atcttatgga
     9121 ccagaaatgc gtggaggtac agcaaactgt gcagtaactg tatctaatga gccagttggt
     9181 tctccactta taacagatga tgcaactgca gctatattat taaatatacc agcatttgaa
     9241 aaattcaaag atgatgttat tcctggtgga aaaataatag taaatagttc tttaataaaa
     9301 gaaaaagttg atagaacaga tgtagatgtt tattatatac cagcaaatga attagcagct
     9361 gaattaggaa atgataaagt tgctaacatg ataatgttag gtgcttactt aaaagtatct
     9421 gatactgtag atatagaatc tgtactagaa gcattcaaaa aagttttcgg acctagaaaa
     9481 gaaaaattcg ttccattaaa cagagaagct ttacaaaaag gtatggatgc tacatgttct
     9541 aaagcaacta actaataaag aaaaagatgt caaaaaaaag acaatagaat tatttctatt
     9601 gtcttttttg ctgataagta ttaaaatatt tttatgactg aaattaagcc tataatgata
     9661 ttttatgaac tataaaaata taatacaaat tacaagcttt tcaattatga aaggagaatc
     9721 tttagtgaga aagtattttg gaacagatgg agttagagga gtagcaaata cagaactaac
     9781 atgtgattta gcatataaac ttggtagagc tggtggattt gtcttagctc aaggagacca
     9841 cagagtaaaa gtagtagtag gtaaggatac tagaatatca ggtgatatgt tggaagcatc
     9901 tttaatagca ggacttatgt ctgttggatg tgatgttatt actgttggaa taataccaac
     9961 acctgcagtg gcatatttaa ctagaaaata tggtgctgat tgtggtgtgg ttatatctgc
    10021 atctcacaat cctgttgaat ataatggaat caaattcttt aataagaatg gatataaatt
    10081 agatgatgaa attgaattga aaatagaaga atatatagac gatatagaca aaattgattg
    10141 tcttccaatt ggtgaaaatg taggaagaaa attacatgaa cattgtgcac aaagagacta
    10201 tgtagactac ctaaaatcaa taataagcac tgactttaag ggattaaaag tagttttgga
    10261 ctgtgctaat ggtgcgtcat ataaagtggc accaatagtg tttgatgaac taggagcaag
    10321 tgtaatttca ataaatagtt caccagatgg aaataatata aactataaat gtggctctac
    10381 acatccagaa caacttcaaa gggctgtttt ggaacataat gctgacttag gtcttgcata
    10441 tgatggagat gctgatagat taattgcagt aaatgaaaag ggtcaaattg ttgatggaga
    10501 ccatataatg atattgagtg ccttaaattt aaagaaaaat aataaattgg cacaagatac
    10561 tttggtagtt acagttatga gtaatatagg gttgacaata gctgctaagg aaaatggaat
    10621 aaatttatct actacagcag ttggagacag atatgtttta gaagatatgg ttaaaaatgg
    10681 atataatctt ggtggagagc aatctggaca tatgatattt ttagattaca acactactgg
    10741 agatggagtg cttagttctc taatacttgc aaatataatc ttgcaagaaa agaaaccttt
    10801 atcagaaatt gcatcaataa tgagccaata cccacaagta ttagtaaatg caacaataaa
    10861 aaatgaaaat aagaacaaat atatggaata tccagaaata aaaactgaga tagaaagaat
    10921 agaatctatt ttagacggaa atggtagagt tttgataaga ccatcaggaa ctgagccatt
    10981 agttagagtt atgttagagg gtaaagagga aggtcaaata aaagaattag caacaaactt
    11041 agctaatctt atacaagaaa aattatcgta atttaaaggg tattctcaga tataaatact
    11101 gaggacacct ttctaagcgc cagaacttag ttatagacat aattattgag taatatagga
    11161 actaagttga cgaggtcaga gttaatcgaa ttgtcggcgg atgctctgcg gtgtgtttac
    11221 catcgaaaga cttctttaaa taataggagc aatcctatgg acaaagggaa gccaaagtaa
    11281 acttatatga cctaactctt aaaatatata aataatattt ttaggaggac acaaataatg
    11341 tgtggaatag ttggatattt aggaagtaga aaggcagcag aagttatagt agaagggctt
    11401 tctaagctag agtacagagg ttatgactca gcaggagtag cagttaatag ttctaatgaa
    11461 aaagaactta atattagaaa gtttaaaggt agactttctg tacttgcaga agacttagaa
    11521 aagaatccta ttgatggaaa tttaggtata gggcatacga gatgggcaac tcatggagaa
    11581 ccatctgatg taaactcaca tcctcatttt aatcaagcca aaacaatagc agttgttcat
    11641 aatgggataa tagaaaacta tatggaaata aaagaagaat taatttctga aggtgttaag
    11701 tttgaatctc aaacagatac agaggtaata gcacatttag tagataaata ctatgagggt
    11761 aatttattag atgctgtata caaaactata tcaaaattaa gaggagctta tgcattaggt
    11821 gttatatgta aagaacatgg aaatgaatta gttgcagtaa gaaaagatag tcctcttgta
    11881 gttggtgtgg gtgaaggtga aaactttata gcatcagata tacctgcatt attaaaatat
    11941 actagagatg tttatttctt agaaaatgga gaagtagttc atctaaaaga cgaaaatgta
    12001 acagtttatg actctaatag aaatcttgtt gaaaaagaag tatttcatgt aacttgggat
    12061 gtagaagctg cgtctaaagg tggatatgac tactttatgt caaaagaaat tcatgagcaa
    12121 ccaacaggag taagagaaac tttagaaaga agacttgatg ataatggaaa tataattcta
    12181 gacagtataa atatttctag agaagactta gaaaaaataa ataaagttta tatagtggct
    12241 tgtggaacag catataatgc tggattactt ggtaagtatg ccatagaaaa atttgtaaat
    12301 ataccagtaa taactgatat agcatcagaa tttagatata gcgacccatt tgtagatgaa
    12361 aattcattag taatattagt tagtcaatct ggtgagacag cagatactct agcagtatta
    12421 agagattcaa aggctaaagg agcaagaata ctttcaataa ctaacgttgt aggttcatct
    12481 atagcaagag aatcagatga tgtattctat acttgggcag gacctgaagt agcagttgca
    12541 tcaactaagg catatactac tcaaataact tctttataca tgatagcttt ggactttgct
    12601 atcaaaaaag gaacaataac tagagaattt tatgatagta tgatttctaa gatgaaagaa
    12661 ataccatcaa aaatacaaga aatactagac aatgaagaat atataaaaga agttgctaaa
    12721 acagttgtta gttctgaaca tgcattctat ttaggaagag gtatagacta tagcttagca
    12781 atggaaggtt cattgaaatt aaaagagatt tcatatatac atgcagaagc ttttgcagca
    12841 ggagagctaa agcatggaac aatagcttta atagaaaaag gaactcctgt aattgccata
    12901 gctactcaag aaaagttatt tgaaaaaatg gtttctaata tggaagaagt aagagcaaga
    12961 ggagcttatg tagtagcaat agctcaaagc cataataagg atgttgagaa agctgctgat
    13021 aaaataatct atataccaaa ttcagatgat attttatcac caatattagc agttgtacca
    13081 atgcaattac ttgcttatca tgtctctgta ctaagaggat gcgatgtaga taagccaaga
    13141 aatctagcta aatcagtaac agttgaataa aaacagatta aaaattaaat agataatatt
    13201 tattgaaaag aactcatcct gttatttgta ttttatagga tgagtttttt agataaatat
    13261 taaatttgtt ttgtttttgc tataataaat tttataaaaa gattgatatt aaatcatctg
    13321 taaaatgata aatatataga ccataccagg tatagggaac gataaatata gttcacataa
    13381 aatatagtga gtacttaata aacatatagt caatatcaga taaaaaaaat aatagcttat
    13441 gagaaaaatt aaaatacaag gtattagtaa ccttgtgata agataaagtg agtagtttaa
    13501 aataagaata taaggaacta tttataaaag aatataatga agctaataga gtataagaaa
    13561 attactttat tttagtaaaa taatttcaaa taaaaatttt tttaaattgg gagtataaat
    13621 tatgacaaat gattttaaaa aagaaaagaa agaagattat tttgttaatc ttaaggcaat
    13681 atctactgaa gttttacaag aaaaagtgca agataacgct tgggttatag ttgatactcg
    13741 cttaaatgat gcttataatg gctggaagtt agatggagta aaaagaggtg gacatataaa
    13801 aggagcagta gatttttctg ctaattggtt aagtgtatac tcagatagaa aagatgaagt
    13861 gttagaacaa gcattgaaaa ctaaaagaat tgatttagat aaaaatatag tattgtatga
    13921 tgcaaatgga aaagatgctt tggttgtagc agattattta tctaaaaaag gatataaata
    13981 tctatataaa tatgatatta aacaatgggc agacgacgaa aatcttccaa tggaaagata
    14041 taaaaattat caaatgatag ttcctgcttt tataataaaa gatatattag atggaaaaat
    14101 tcctgagaca tttgaagatt caaagaatat aaaaatgatt gaagcgagtt ggggagaaga
    14161 atcgtataca aaagggcata tacctacaag tgttcatgta aatactgata ttatagaacc
    14221 tcctccaaca tggatgcttg ataatgatga taacttaact aagtttgctt tagactatgg
    14281 attaacaaag gatgatactg taatagtttc aagtagtaca cctatggcat cttatagact
    14341 agctgttata ttgagatata taggagtaaa agatgtaaga gttttgaatg gtggaacaaa
    14401 ctcatggtta tcagcaggat atgaattaga atttattagt aatccaaaac attcatgtac
    14461 taattttgga gcagatatac cagttaactc acaattaatt gttactacat ctgaattaag
    14521 acaaaaacta aaagaaaaaa ataagtttat actagtagat aaccgtactt gggatgaaca
    14581 tataggaaaa gtatctggat atacatatta tgataaaaaa gggcgtatac caggagcttt
    14641 atatggacat tcaggtagtg atagtgtttc actagaagaa tatcgtaata ttgataatac
    14701 aatgagaaat aaatatgaga ttttagaaat gtgggacaag gaaaatatag atgtgaataa
    14761 acagcttata tttatgtgtg gaagtggatg gcgtgctgct gaagtattaa catatgcaaa
    14821 tgtcatagga gtggaaaata cctcccttta tagtgatgga tggatgggct ggagtcttga
    14881 taattcaaat ttaattgaag tgggagaaca caaataaagt ttttgaaagt gcaatagagt
    14941 tgtttaaaac aaaggtgttt ttgatttatt aggaagagtg tgtaaattgt gtattgggaa
    15001 gtgtacttag gtatgttcat ttggagctat ggaaattgat aaaaaagtat tatctgttta
    15061 ttttgttggt aaaatatgaa tagattatag attatagatt atagatttaa gtatagacta
    15121 agtaatttat attacagaag agaatatata agaattaatg caagatgtat ttgatacata
    15181 taagaaattg gcaagtccag gagaaggata gagaaaattg agattgaaga gtttgaaaga
    15241 gattattaga tgaaaaatac aaaaatgtat aaaaatacat attgagaaag tatatctctc
    15301 ttaaatttgt taataattct attatacaga aaaacatatt taagggggat tttttactat
    15361 gaaaaaagta aaattagcta taagcttttt tatattaatg attataggaa tgacaa
//
LOCUS       DAEMGG010000095        15402 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEMGG010000095 DAEMGG010000000
VERSION     DAEMGG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.095"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            156..2390
                     /locus_tag="KPK24_002710"
     CDS             156..2390
                     /locus_tag="KPK24_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2300364.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2421..3254
                     /locus_tag="KPK24_002711"
     CDS             2421..3254
                     /locus_tag="KPK24_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2300365.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKKKFDKND
                     Y"
     gene            3494..5227
                     /locus_tag="KPK24_002712"
     CDS             3494..5227
                     /locus_tag="KPK24_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2300366.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENTQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5619..5981
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5688..5716
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6511..6615)
                     /locus_tag="KPK24_002713"
     CDS             complement(6511..6615)
                     /locus_tag="KPK24_002713"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG2300367.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7240..7485
                     /locus_tag="KPK24_002714"
     CDS             7240..7485
                     /locus_tag="KPK24_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300368.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIVFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8238..8384)
                     /locus_tag="KPK24_002715"
     CDS             complement(8238..8384)
                     /locus_tag="KPK24_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300369.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8480..9129
                     /locus_tag="KPK24_002716"
                     /pseudo
     CDS             8480..9129
                     /locus_tag="KPK24_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
     gene            9670..11025
                     /locus_tag="KPK24_002717"
     CDS             9670..11025
                     /locus_tag="KPK24_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2300370.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11143..11811)
                     /locus_tag="KPK24_002718"
     CDS             complement(11143..11811)
                     /locus_tag="KPK24_002718"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG2300371.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(11878..12306)
                     /locus_tag="KPK24_002719"
     CDS             complement(11878..12306)
                     /locus_tag="KPK24_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG2300372.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12413..13006
                     /locus_tag="KPK24_002720"
     CDS             12413..13006
                     /locus_tag="KPK24_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG2300373.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13125..14060
                     /locus_tag="KPK24_002721"
     CDS             13125..14060
                     /locus_tag="KPK24_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG2300374.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(14331..15275)
                     /locus_tag="KPK24_002722"
     CDS             complement(14331..15275)
                     /locus_tag="KPK24_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG2300375.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
BASE COUNT         5844 a         2028 c         1951 g         5579 t
ORIGIN      
        1 tgtatttgta gcattggagc aagtaactta atgtatattc attttaaata cttatttaaa
       61 atttagcatt taaattattt acaaattaac atttaaatac ttctagcata aatacagttg
      121 tcattaaaat ttaaagtatc ttatggagga ataccatgca aaatcataat tatataaaaa
      181 tgttaaataa aaaggtagaa gtattatcta aggaattaga tagtagaaat tttaaattaa
      241 aaaccttaaa tgaaatagat tttttttcta aaataccaaa cagagaattt atgtaccaac
      301 atattgatga ctgtatttcg aaaaatatac atataggaat catgttatta aatatcgata
      361 actttaaatt atttaatgat atatatggat acctagtcgg agaaaattta ttaattactc
      421 taactcaaaa cctattgcac tttattccag aatcaaaggg aatagtcgct tatttaaaag
      481 gagaaggttt tttaattgta cttccaaatg tatcaaataa agagttaaat aatatgggtg
      541 caactattat agagagcgcc cgagaattaa aaataaaact tcctaatgaa aaagaaatat
      601 actccaatgt tacatttagt atcggcagca ctatctggaa tggtgaacca aacatgacta
      661 ctttgatgct tttcaaccaa gtatatagtg ctttaaaaaa ggctaaaagt gaaggtaaaa
      721 atagatacat tctatttgat tttaaagata atagttttta caataaatta gaaaatgata
      781 aggagttctt atcagaaata tcacatgttc tacaaactaa acaaattgaa gtattttacc
      841 aaccacttta taatataaaa aataatacta tagttggatg tgaggcactt gcaagatgga
      901 gacaccctat taaaggactt cttactccag ataaatttat tcctttattt gaacagtttg
      961 gtctgataac ggaattagat ttgcatttat ttgaagagaa ctgtaaaaat ataagaagat
     1021 ggattgataa caatgatgtt tttgttccaa tttgttgtaa cttttctcgt cttcattttt
     1081 ttaaccctaa ttttccaagt atgctaaaag gtattactga aaagtacagc gtctctacat
     1141 caaattttag cattgaaata acagaaaata tattaataga aaatacaaat acaattattc
     1201 aacaactagc tgaacttcgt tcttatggtt tttctgtatc aatggacgat tttggttctg
     1261 gatattcatc ttttggtatg ttgcaaaatc taccttttga tataatcaaa attgatagga
     1321 tatttttatc tcataattta aatgatttta aaaatacaac cattttgtac tctataataa
     1381 acattgcaag agctcttggg atgcttacag tatgtgaagg tgttgaaaca gaacaacatg
     1441 tagaatttat taaaagtata aattgtgata tcgcccaagg ttattattat gcaaaaccca
     1501 tggagtgcaa agaatttgaa aaattactat ctaaagagaa taattcagag aaaatgttta
     1561 taaaatattc tgatatagga aaatctttaa tagagaagat atttgatgga ttttttatta
     1621 tgcaagatat gaaaaaacta agagctaatg tcactgaaga cattcaatgg tttgattttt
     1681 acaacaaaac tgaaattaca tcttttccta aagtaataga gcattttgaa aaacatatta
     1741 aaggtaaaaa attttctatt ctttacaaaa gtataacccc acataatact gaatcagatt
     1801 cattattaat tagtggagaa gccgttttat cagatgaagc ttctgaacat caaaaatata
     1861 gcaatttcta ctttagtgca aattgtataa tttcagaaaa taaattactc ttagcaaaat
     1921 tacatttgga tttaattaaa actgctggct atataaatga atttgaatca tattcaatac
     1981 ctaaaatgac aaatacacat cttgatgaaa gtaatatttt atcacaattt tattcaatgt
     2041 tgcctgtagg tataattcgc tacgatttat ttggtgatat gattattact tatatgaatg
     2101 aagaaatgtt tgatattata ggttatacaa aagaacagtt ttttggtgag atgggcggaa
     2161 atttaagagc aattgttcat ccagatgatt tagactttgt ttataaaaaa agtttggata
     2221 tgattgacac aaataaaatt gaaccatttt tatatagatt cataaaacga gatggtcaaa
     2281 ttgtcacagt tatgtataca caatgtaacc tttctgcaat tgatggaagg tcgattgccc
     2341 agggtatgta tgtagatgta aataaaataa aaaatttaac aaaaatctag tacaaagtat
     2401 tttattttgg agaaattaat atgtttaaag aaattttttt aagaactttt ccaggatttc
     2461 taattataag agattctaac tacagaatta tatttataaa tgataattta aaaaatttaa
     2521 ttaaaagtta tatgcaagat aatccacttg gaatgactaa tatagaaata gctaagaaac
     2581 tccctgataa tattgctaaa ttttttacgg attctcacaa tattcaatta gattgggaaa
     2641 aaaattatcc ttatgataaa atttcaaatt ggattttaga gtttaaaaaa gatactacaa
     2701 gctattggaa tgttttagaa tataaagtag atgttgatga aaaaacctac ataattacta
     2761 tggccaatga tataacaaaa ctctatgaag aaaataagag aaatcttcac tactcaatta
     2821 ctgacccatt aactggagca tataatagaa aatatttaaa tgatagattt gacatattta
     2881 taggagatta tattgtacta attgacttag ataatttcaa aatgataaat gactatgaag
     2941 gtcataatgt aggagacaag attttatgtg attttgtttc acttttaaat aaagaattaa
     3001 taaattctac ttctattata agattaggag gagatgaatt tattgttata ttctctagtg
     3061 atgttgacaa aaattatgtt tattcacaac tagaagcctt aagagaaaat tttttaaaag
     3121 tattctctaa gtataagtat ctttcattta gttatggtgt tgatacagta aaaagaaatt
     3181 taaaattgac aatagttgaa ttggataaaa aaatgtataa aaacaagaaa aaatttgata
     3241 aaaatgatta ttaatattgt tattttaaaa taaattaaaa ttatctttct ttttttaaac
     3301 ttgtacatta gtttataaat atgtatctat acaagatatt gacattaagt actgaagcat
     3361 atattgtttc agttcttttt tgttaaaatt aacttattta acaaagatat catagtttaa
     3421 caaagctttt aatttaaata tattgatgta taatataatt aaaatataat agcaaaaaga
     3481 aaggaggaat atcatgagca tactcctaaa aaaagcaccc aaattagcaa aacatattat
     3541 aacaagtttt tatataaatc gggatattga tgaagtatta aaataccttt gtgaaaatgt
     3601 tacatggatc ggaccaggag aacaggaatt tctcacttct tttaatgaaa taaaaaatta
     3661 tttttatgca ggtcaagatg aaattccttc ttgtgatata aataatgata tatttgaaac
     3721 tgtaagtgaa gatgagaata gatgtatggt attaggaaga tatactgttc gaactaaaga
     3781 aaatactcaa atgattttag aggtaaatca acgttgtaca tttgagataa ttgaggatag
     3841 aggaaaatta ttagtaaagc acatgcacat atcaaaccct tatggtgaaa tgcaacttga
     3901 tgaatacttt cccactaaaa ttggaactca aagttatgat tatttgcaaa gattactaaa
     3961 ggaaaaaaca gaagttattg aaatgattac aaataatatc aatggtggct taaaaggtag
     4021 caatgatgat aatacatact catttttcta tgtcaatgaa ggattgccca aaatattagg
     4081 atacacatat gatgagttca tggaaatgag tggtggttca gcagtgggtg ctgtatatcc
     4141 accagactta cctaaagcac tagaagattg tgaacgatgt tttgcaaaag gacctacata
     4201 ttcatctgaa tatagaatcc gaaaaaaaga tggaacatta atgtgggtat tagattctgg
     4261 aatgaaatca ttaaatagtg atggcatagt aaaaattaat agtattatta ctgacataac
     4321 ccaactaaaa aacatagaat ctgaactaaa attagaaaga gagcgatatc gtattgcttt
     4381 gcaaaatatt acagatatca tgtttgagta tgatattgaa aacgataccc ttataaaata
     4441 tcaacgtgta gaaattgaca aaaaaattga attagaaaat tttgaaacaa aaaattactc
     4501 caaactatta gaatctggta aaattattca ccttgatgat attggaaaac tactagaagt
     4561 acttcatgga aatcttcgtg aaacaattga gataagacaa gtaaattcat taactaaaaa
     4621 tgaatggaga tggattcgtg ttcaatgttc agttatatat gatagtgacc ataaccctat
     4681 caaaacaata ggcgttttaa aagacataac tgaagataaa tctaaattag aaagcttaat
     4741 taatcaggct caacgtgacc cactaacaca attatataat caaagagtat cacaaaatct
     4801 tattgaagag tatctatgta gttctgattc caaaaataat gatgctctat tagttattga
     4861 tattgatgat tttaaaactg taaatgatac ttttgggcat ttagagggaa atgaagtttt
     4921 agttgctgta tcaaaaatac tattacataa cacacatgat aaagacatag ttgctcgaat
     4981 tggtggagat gaatttacaa tatttataaa atcattaaca aaggatttaa taataaaaat
     5041 tactaatgat attttaaatg atgcaagtaa aataaaagta aaggacaatc ataaaataac
     5101 attaagtatt ggtattgcct tcactgatga ttcaactaaa ttatataaag atttattttc
     5161 taaagctgat aaagctcttt atctatcaaa agcagatggc aaaaattgtt atagtacata
     5221 cgaataataa agctatttat acgaccaaga atgaaccttt aaatttaatt tcatagtcta
     5281 acaatcaaag agttgaagct aatcttatag ctagtcaact agttcctaga aaaaatttaa
     5341 acaatatatt tattgatact gctcattttt ctaaagagca aatttatata aaaaattaaa
     5401 cttttatgta catacagata ctaatgataa acagttatca ttagtatctt ctatcttaaa
     5461 acaaaatgat aatttatcta tataaaaaag tgctttgttg acagagttca cttttttata
     5521 taggtacaga atttaagtat aaatacatgt tttattctat acattttagt taatgtaaaa
     5581 tttcttgatt gtactctcta acttttcaac attatactat ttatatacca cttagttaat
     5641 ataaacccct tagctggttc tagtgctttt tctatgattt tagtcatatt tacataccac
     5701 ttagttaata taaaaccaat ttagatgatg tatatgtgca aaaggaagaa ataatttaca
     5761 taccacttag ttaatataaa acgctactaa acttgatata catgaccatt ataataataa
     5821 atttacatac cacttagtta atataaaact tccataaaaa catccccttt ttattcttct
     5881 tccttatatt tacataccac ttagttaata taaaacatat tttggattta ttaatataaa
     5941 cataattaaa aaatttacat accacttagt taatataaaa ccccaaaata aactgggcat
     6001 tttcaatact tacacatatg caactctctt aaatttgcag tgaaccatga gtagtgcaaa
     6061 tgataaagct tatcatatac cctcaacacc ttatatttca agcgttaagc tctactttgt
     6121 aacaaatatc gctcactgca aaactattta catttttatt atatcataaa tatattattt
     6181 ttgaatatct ataccaattt gtggtataat aaaagcaaga agaactacaa tctatttaga
     6241 cgtagagtga agttcaaata ctaatgttta ttctttttga acttgatttt tagttcaaaa
     6301 ctaaagtcac tcctgcctgt gtgaactata cccgctgttt tatacagtta aaacatgtaa
     6361 aaatagttat aaagtactag tgtaactcat tagtactctt tttattatat tcatataatt
     6421 aatatctata ttttaaagtt tactatctaa aaaagagcac gtcaaataaa accctagata
     6481 tttaattttt ttagagatac ttaaaataaa ttatgctctc tctgtactta aatatttcct
     6541 aaattcaaat ggagacatag aatttaatct attttgatat ctaaaattat tatagtagta
     6601 tatataatca tctattgctt taactaatta ttcatatgta tcaaatttcc ttaaatagta
     6661 catttctacc tttaaaattc cccaaaatcc ttccatagga gcattgtcaa ggcaacgacc
     6721 aactcttgta taatatatgt atatctaaca ccattaagaa atatatattt tataatatat
     6781 atttcttcat ttaatgcact tagaaaagca aagaaaatac agattagtac actttttaga
     6841 gaaaaataaa attaatacat ctaaaatatt aaaaaatact ttagataatc taaagacaca
     6901 tctcaaagtt tttaactaaa ttataaaact aattacaaat gctatgtaac gataataaat
     6961 aaaaaattta cttcgcttaa gcgacgtgtc gttgaaatat ttcatgttgc caactggttc
     7021 gtaagttgat aagaaaagaa cactaattat ttagagttct tttcttagct acaagtggat
     7081 tataatactt ttaaatctgt gagaaaatca tatataataa tgatatgaac aagtaaagaa
     7141 aatacaaaaa tgacaaaatt ataaataaaa tattataaaa tcataaggtc ataattgtta
     7201 tattgtagaa acaaagtaaa gagagagggt ggagaataaa tgaatcaaaa aaaaataaag
     7261 attagtatta aaatggtaat tataggtata tttgctgtaa ttattgcttt tgtattatcc
     7321 agattattcg ttgagtatat tgatgaatta ttaaggtcta attggcaata tcagctattt
     7381 gaaagtgaga gttcatatat agtatttgcc aattgtatta tgttctcgaa tatattatta
     7441 gaaatatttt taatatatat ttgtagaaaa tttagaaaaa tctaatatgg tcaacgtatt
     7501 tcgaatcgaa tacttcatga ttatttagtc tattgtgcta gttaaagttt aattcccata
     7561 taaagtaaat tgattttagt aaaacaaatc acccctaagg gttagtttat ggataagtca
     7621 tccaaatatt aactctttaa tttttgctat agattcatct ttgctcataa ctttacttat
     7681 aaaaaatgaa atattataag ctagaacttt tattctaatt ctagtcataa atcctagcat
     7741 agagttactt tttttatatt ataaataaaa ctatacaaca aagataatat ttagtaaaaa
     7801 taactttcat tttaactaaa cttaaaattc aattactctc taaattgacg tttaggaatt
     7861 agttcaaaat aaaaaagagt gtatatattg aaattactac atttcaatat atacactctt
     7921 tttaaatttc tcagttttcc atggaacttt actttattta tttctgtatt agttgtattt
     7981 tttatgcatt tttctttaaa atcatatttt ctgattcaac tcttatatta taaaatttct
     8041 ttactaaatt attctctttt tctatatctt catatatatt ttttaatcgt cttttaccta
     8101 atcttttatc taacattact aatgatttaa ctaatatatt atatgatttt atcgcatctt
     8161 ctattgagat atttaaacat atatttactg cttcataaaa atcaaattca ttaaaaaatc
     8221 catttttatt tacaatctta tatccctttg catctctttc taaatactct tcctaaatac
     8281 tcttcaaaac tttcatcatc taatctaaat cctatatcac tatgaaaaat aggttttgca
     8341 tttggatata gttctattgt tttatcaaaa gtttcgaata ccaagttatt attattcgat
     8401 tttccaacag tccactataa acaataataa gtttgttgct ccaaataatg taacacttgt
     8461 agaaagatgt tacagatata taaaagatag agaatgtagt ttttctccta atactattag
     8521 aaatagaaaa aatatgatta aaaatcaaat tgaagttttc tttaaagata tgaaattaat
     8581 agatataact ccttctattt tacagtctta tataaataac atatacaatg agcacatgtt
     8641 aaattcaaca aaaaaccaag tcgattttat aaaatcagta ttgaaagaat cttatagatt
     8701 aaaagaaata tcagaaaata tttgtgattt tgttacaacc ccaatcaaaa aaaatagttc
     8761 aacttctaag ctttatacaa aacaaaaagc tcaattatta ttagaaaaat caaaaaatat
     8821 tcctataggt ataccaattt ttttgatgct aactcttgga ttacgttttg gtgaagctgt
     8881 atcacttata tggtcagatg tagaccttga taaaaaatct cacatgtaaa tcaaacatta
     8941 gtttatgtat acaataaaat aatcttcaaa ggccctaaaa cccctagatt caaaagaaga
     9001 ttatttgttc ctgatgaact tataagtttg tttaaagggg aaaaattaaa acaaaataag
     9061 ttaaaattac agggtatatt gaaaaatgaa tttgatttga tatgcttaaa taaaagattc
     9121 aacccgtgag ttcaaccaac ttttgttgtt cattttaaaa aactattaag agataataat
     9181 cctagagata taaaagtaca tgaattaata catgcaaatg cgactttgat attactttca
     9241 ggtactaaca taaaagctat ttataaaata ttagtacatg cagatataaa aatagctatg
     9301 aatatatatt ccatattctc acttattaga tgaaatgaat aaagaaacat ctgaaaatat
     9361 aagcaaaata ctattcaaat aatgttcagt tttatgtcaa ttaaatgtca ctcaaagtgc
     9421 tttttttcca tttgtcagtt aaatgtcact taaagtacct ttttcctatt tgtcagttaa
     9481 atgtcagtaa tatataaata agatatattt ataagaccct taaaatcact attatttaat
     9541 aaaatttata attatatact atttgctgtt gacattggta ttataccata gtttatcatt
     9601 tatattagaa taaaattatt aattaatagt ttcgtaattt tataatagat ttacagggag
     9661 tgataataaa tggatacaaa aatatctata aacgaaaaca tgagtctagg taaaagattt
     9721 tttaaatacc tagcaccatc tgtagttgca atgtgggttt tctcactata tacaatggtt
     9781 gatggaatat ttgtaagtaa aggagttgga gaattagctt tagctgctgt caatatatca
     9841 atgcctttta ttaattttat atttgcagtt tctctattat tttcaactgg tgcatctacc
     9901 ataattgcaa tctatctggg gaaaaaagat attaaatctg ctaacgaagt cttttcattt
     9961 aacttagtat caataattat attatcaata attatattag ctataacttt ttttaattta
    10021 gatagattag cactattttt aggagctaca gaatctacaa ttggtatggt taaagattat
    10081 ttaggaataa taatattttt taacggattc tttatagtgt cttattcttt agaagttata
    10141 ataaaaactg atggctttcc aatattagct actgttggtg taataatatc tgctcttaca
    10201 aacatcatac ttgattactt gtttgtaatt gaatttggat ggggtgtaaa aggtgctggt
    10261 attgcaactg gtttgtctca agtcttttct actattttct tcttaataca ttttttaaga
    10321 aaaaactcta ctttgaattt ttcaaaattt agaattgatt ttaagacact tagaaaaatt
    10381 gtatttatag gttttcctga ttcaactact gaattaagtt gtggtatagt tgttttactt
    10441 tttaacttaa gtcttactaa atatattgga gaaaatgctc ttatttatta tagtgtcata
    10501 aattatataa atacattagt acttatgact atgatgggta taactcaagg tatgcaacca
    10561 cttactagct tttattatgg tgcaggaaat atagataatg taaaaaaatt attaaaaatg
    10621 gggataaaag ccacaataat agcatcagtt gctgtatttg caatttgtat ggctttttca
    10681 ggaccaatag tctctttatt tatacatcca gaagaaacaa tgctattcaa tgaaggagtt
    10741 cgtgtattta aaatattctc tatatctttc ttattggtag gaattaatgt aataatttct
    10801 ggattctttg tatcagtaga aaaaccatca atttctactg ttatatctct tggtagaggg
    10861 cttgtaatag ttgttttaag tctaatttca atgatactaa tatttggtgg acaaggaata
    10921 tggatgacta ctattgtttc tgagtttata tgtcttattt tatcattagt ttttctaaaa
    10981 aagaattttt ctacattaga ctctaatcta aataaggttg cttagatgat aagagtgaca
    11041 atatatgaaa atatctaaat aaaaaatacc tattatacaa tcttgcaaat attaaacctt
    11101 tgcaaaaatt aggattatat aataggtatt ttatttatgt aattatttta tttctaaata
    11161 tccatagcca tcttttatta aagttccttt tacttcatat tctacaggtt ttttaaatat
    11221 tggaccatct acgacaaatg tatgatactc tccatgttct ccacatgggt ctgcaccagc
    11281 attttttatc ttctccacaa gttctctagt taattttttt cctaaaaatt cctctcctaa
    11341 gtgctttaag tttacttttg ttacaacact acaaaatcca ctgtctataa attcatatac
    11401 taaatcctct ctactctctt gccataatgg taaatctact ttcataccaa ctgcttcaca
    11461 tctatcagta tcccattttc tatgactttc tatatctata tctccatatg cacatatcgt
    11521 tgctcccatg tttttagctt ttattaaagc cctctcaaag ctctcttcat atccttctac
    11581 gccacactca gcaactaata aaggtaatcc tatgttgcta cttacttgct taagcatatt
    11641 atattctaaa ttatgtgtcc atgactttcc atgttcttca cttatagttg tcaagagtgc
    11701 cacaggctta tatccttttt tcaacattcg atttaatgct aatatactat ccttaccacc
    11761 actaaatgac attacaaatt tttcatcagc caattttaac acctctttca tctaactttg
    11821 ttttattaac ataattatac caaatttttg agttgcatag caaattatat taaattgtta
    11881 attcactata aagaatgtcc ctaatattaa aagtataacc cctatgtatt tgtataaatg
    11941 cattgtttct tgtcccaaaa ttacgctatc aataaaaaat ccaactaaaa cttgggatac
    12001 aagagttaat ataagtgtat ttgatactcc taaatttgga attgccttta ctgtaaaata
    12061 tattattaga cctccaaata ctcctcctaa taacaactta taattaaaac catttataga
    12121 tactagacac tttaagtcac ctgtaactat tatacttgcc aaaaaaaata ttgctcctac
    12181 agttaagctt ataaaggtgg ccactaatgc tgttgtactt tttcctagct ctccatttat
    12241 aaaagcttca agagctgtag aaccacctgc taataatgca aatattattg cccataattt
    12301 tgtcatggac aaacctcctt ctatataatt ctattaatag aagtttatga tacaacctat
    12361 taattaatta ctaagtatag tttaattttt atacactagg ctttatttgg atatgaattt
    12421 ctgttataat tgttctatcg attttggagg tgattttatg tacacaaatt ttaatatcga
    12481 ttttaataac tttaataaaa aagaaaatgc tattaaattt atgttaatgg gagtattact
    12541 cattatatta ggattattat gccttacatt taagacatta ggtataaaat taatttcttg
    12601 gacttttggt attgcacttt tattctttgc atatttaaat ttaaaaaata ttaatgagtt
    12661 aaaaagatat gctactaaag aagaaattaa accctctatt aatatccaat gggttctaat
    12721 aattgcatgt atattacttt ttgtatttcc tcaaaaaata caaagcatat tttcattatt
    12781 tttaggattt tatttaatct ttaatcaact agtagcactt gtaaatagta agaataatcc
    12841 ttattctaaa tttacaactt ggaatatagt taagattcta tttggtatat gccttatatt
    12901 atctccactt tttttatcta gatttatagt atcgataatg tcttttttca taatattatt
    12961 tggactagtt ctatttttct ctggaaatac agcgagaaaa tattaattat tttctcgctg
    13021 tatttttata ttacttaaaa tagaatttgt ttaagttatc tatttgtggt aatatgtttt
    13081 ttgtacaagc tcagcgtaca attttattag aaataggtga ttttttggat aaatttaaat
    13141 atgcttttga taataaaaga tatcatactt ggaattacta tcttagaaat acttttggag
    13201 aaaaagtttt taaggtatct ataaatgcag gatttacttg cccaaacatt gatggaagtc
    13261 ttggttatgg cggatgtacc tattgtagca aagaaggctc aggtgatttt gctggaaacc
    13321 ctaaagataa tcttatcagt caattttata atataaaaga aatgatgtta aaaaaatggc
    13381 ctcatgcaaa gtatattggc tactttcaag cctatacaaa tacgtatgct cctcttgaag
    13441 ttttaaaaga aaaatatgag actatattag aacttgaaga tgtcataggt ctttcaatat
    13501 ccacaagacc tgactgtttg ccagatgatg ttgtagaata cttatctgaa ctaaataaaa
    13561 gaaccaattt atgggttgaa cttggacttc aaacaattca tgattctaca tctaaaatta
    13621 taaatagagg tcatgattat aaaacatttg ttgaaggtgt tgaaaaacta aagtctaaaa
    13681 atataaaggt tgtcgtacac attataaatg gtcttcctgg tgaagattat aatatgatga
    13741 tggaaacagc aaaggcagtt ggtaaaatgg gtgtagacgg tataaaaatc catctattgc
    13801 atgttataaa agatactcct atggaaaaga tgttacaaaa tggtatgtta actcttatgg
    13861 aacaagatga atatatcaat cttgtctgtg accaattaga aatacttcct gaaactatga
    13921 taatccatag actcactggt gatggtaaga gggatgaact agtaggtcct atatggagcc
    13981 taaaaaaatg ggaaatttta aatcaaattg atgatacttt aaaggctaga aattcttatc
    14041 agggctgtaa gtttgtttaa tacaaaataa taactataga aatggttata taaatatagt
    14101 cctttctata gttatttagt atttacaaaa caaaatatta atttgtacat ttatacaata
    14161 tataataaaa aatagatagt ctaaactgta tctacttata ttttatttgt actgtccact
    14221 ttattctata aaatttataa atagttattt tttaatacca aatataattt ggtataattt
    14281 ttgttggact ctaaaatgta taccaataaa tcctttttaa gtatataatt ctaaatactt
    14341 tcagtttttt caatctgctt taacttgttg gacttataaa atattcttat ttcatcataa
    14401 gaaagattaa gtgctactac tgcagatatt aacatcaaca tcactccaaa taacttccct
    14461 aaggaaaaac tttctcctaa tatagtgcaa ccaattatta ctgaaccaac taattctact
    14521 gatgccacaa cacctgcaac tgacaactct acacctttag atatacctgc tgcataaaaa
    14581 atatatgcaa gtgcagctgg aacaatgcct aacattagca tacaagataa aacatctaaa
    14641 tccttagcat aatttaagat ttcccaaggt cttgaagaag gaatcataaa tatagctcca
    14701 aataagaaac tgtatactaa tattgttgaa cttgaaaatt cctttaaagc atttttacta
    14761 atagttggca taagtgcata tgctattgct gataataccc caagtaaaag tccaaaagcg
    14821 tttaaacctt gaaaatccaa acgtccacca gtcactgcca taattgcacc aataaaacaa
    14881 agaataagtg aaaataattt gcttctagta atattttctt tataacatat ttttgaaaat
    14941 attgctaaaa acagtggtga tgtatatagt aaaactgctg caattgatac acccgcaatg
    15001 tcaattgctt taaaataaca taaattaaac attgcctgac atataatacc tattattact
    15061 gaatatataa ttcctttttt gcttatcttt aagatttgtg gtgtccttat agatgaatat
    15121 acgcctaaaa ctataaatcc aaaaaacaat cttgtaaatg ctacttgttc tgatgtcagt
    15181 ccatttccca ttaaaaactt tccaaataat ccaagtgtag cccataataa ccctgctatt
    15241 gctataaata catacccttt acttttttct cccattttaa attcccttct atttttattt
    15301 agtcaattta cactcttctt tttaagcaaa aactatgcca tcttaaatca atatatatcg
    15361 actctaaaac cctagtcttt ccaataatac taataaattt tc
//
LOCUS       DAEMGG010000096        15138 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEMGG010000096 DAEMGG010000000
VERSION     DAEMGG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.096"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            60..1553
                     /locus_tag="KPK24_002723"
     CDS             60..1553
                     /locus_tag="KPK24_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2300376.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYNSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            1627..1860
                     /locus_tag="KPK24_002724"
     CDS             1627..1860
                     /locus_tag="KPK24_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300377.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            2016..2780
                     /locus_tag="KPK24_002725"
     CDS             2016..2780
                     /locus_tag="KPK24_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2300378.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(3065..6244)
                     /locus_tag="KPK24_002726"
     CDS             complement(3065..6244)
                     /locus_tag="KPK24_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2300379.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPREYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            6525..8702
                     /locus_tag="KPK24_002727"
     CDS             6525..8702
                     /locus_tag="KPK24_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2300380.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFTNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            8716..9138
                     /locus_tag="KPK24_002728"
     CDS             8716..9138
                     /locus_tag="KPK24_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2300381.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            9546..10349
                     /locus_tag="KPK24_002729"
     CDS             9546..10349
                     /locus_tag="KPK24_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2300382.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            10342..11010
                     /locus_tag="KPK24_002730"
     CDS             10342..11010
                     /locus_tag="KPK24_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG2300383.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            11246..11908
                     /locus_tag="KPK24_002731"
     CDS             11246..11908
                     /locus_tag="KPK24_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG2300384.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            11968..12765
                     /locus_tag="KPK24_002732"
     CDS             11968..12765
                     /locus_tag="KPK24_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2300385.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            12883..14073
                     /locus_tag="KPK24_002733"
     CDS             12883..14073
                     /locus_tag="KPK24_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2300386.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            14182..14931
                     /locus_tag="KPK24_002734"
     CDS             14182..14931
                     /locus_tag="KPK24_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG2300387.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         5859 a         1607 c         2501 g         5171 t
ORIGIN      
        1 tataataata taatgtagaa aaatatatag ataagataaa tataataagg tggtaatata
       61 tgaaatatac atttgaaaaa tttaagttaa atgaaaagat acttaagtca ttgaagagtt
      121 taggatataa tataccttct agagtacaaa gagaagttat tccaaaatta ttaaaaggtc
      181 aaaacttagt agttaggtca aaaacaggaa gtggaaagac agcaagtttt gcaatacctt
      241 tatgcgagaa tataaatgta gattacaata atattcaagc tcttatagtg gttccaacaa
      301 gagaattagc cttgcaagta aaagatgaga tatcagatat agggagacta aagaaggtga
      361 gatgtagtgc tatatttggt aaacaatcta taaaagacca aatagctgaa ttgaaacaaa
      421 gggttcatat tgttgtagct actccaggca gaatacttga ccatataaat agaggttcta
      481 ttaagttgga aaatgtaaag tatttagtaa tagatgaggc agataaaatg tttaataaag
      541 gttttgttga acaaatggaa aaaatattat taaatttacc taaggagaaa atagtaagtc
      601 ttttttcagc tacaatagat gaagagataa aatatatatg tgaaaaatat atgctagatt
      661 acagtgttat aaatatagaa gaaaatgaga gcgatactaa tcaaaaaaca agacaaatag
      721 atgacaaaat aatcaaggca aatggaagag agaagtacat ccttttaaaa gaattaatat
      781 acagcgaaaa tccaaagagt gtgataatat tttgtaatac caaagagaaa gtatctaaat
      841 tatataacaa gatgagtaaa gaaggttttt taataagaga gttacatgca gatttatcac
      901 aagaaagaag aatttttgta attaaggatt ttaaaaacca aaaatttaat gtattagtta
      961 gtagtgatgt agcgtccaga ggaatacata ttgatgatat ttctttagta ataaattatg
     1021 atgtgccaca agataaagaa aactatatac atagaatagg tagaactggg agaaaaggaa
     1081 atagtggaaa ggcaattact atagtaactg aaaaggatga aaaatatata gagaatatag
     1141 aaacgtatat aggatataaa ataaatgaat taaaagatat agaaaaaagt agaattacac
     1201 atggcaaaat tttgtttgaa gaatattcaa aaggaatcct aaaaaaagta tcaaagagaa
     1261 aaaaagttga taaaaattct tacaatagta aagatataaa actaaatata gaaagtgaag
     1321 ttgttaaatt atatttaaat gctggtaaga agaagaaaat aagagtttta gatatagttg
     1381 gtgcatttag taatataaaa ggtataacta atgatgatat aggtgtaatt gaagttcaag
     1441 atttatgttc atatgtagat atattaaatt ataagggaga tttgatatta aaaaaatata
     1501 aggagatacc tataaagaaa aaaatggtta aagtgaaaag agatatacga taatttagtc
     1561 aatattgatg aattgctttg ttatcgaaat gaagttgcaa aaaacacaat agaagttgtt
     1621 aaggatttga aagctgaaga tatgaaacga aaggtctctc caattgactt agaaaggatt
     1681 ttggtgactg gaggagtaat aaagcatgaa gattctattt ggttgttgga cttttggggg
     1741 aataaagata ttgctggaat tttgcttatg cctcctactc gtcatgttat acttcatttg
     1801 aatgattgtt gtaaatggaa ggaatatttt cgtacaaaaa agaaatttta cagaagttaa
     1861 attataataa attagacaaa caatagataa attagcaagg aatgttgatt ttatctacat
     1921 tctaagttat actattttta agtggctaat ggaatagtca ttttaaatat aagtaataaa
     1981 tatataccta tagggatatt aaagggggga aatatatggc atattttaac tataacgaca
     2041 aacagtgcta ctataatgaa attggggatg gtacaccatt acttttttta catggaaata
     2101 cagcctcttc caagatgttt aatgatataa tcgattttta taaagatgaa tataaagtaa
     2161 tattaattga ttttgtaggt catggaaaat ctcaaatggt cgataaattt tctgctgact
     2221 tatggtttga tgaggctatg caagtgatat gttttttaga agcaatgaac tataaaaaag
     2281 taaacataat tggtagtagt ggaggagctc ttgttgcatt aaatgttgca cttgagagac
     2341 ctgatttagt gaataaggta attgcagata gttttgaagg agaagttcct ctagagtcat
     2401 ttgtacaaaa tgtgaaaata gagcgtgaag catctaagca agatgatggt gcaaaagctt
     2461 tttatattta taatcaagga gaaaactggg aaaaagttgt agataatgat acagaagcta
     2521 tttttgaaca ttataaaact ataggaaaat tttttcataa gcctctagaa actatgcaac
     2581 cagaggtgct tttaacagga agtagagaag atgagtttgt atctctaata tctagtgatt
     2641 tttttgaaaa tactttttca ttacttcttg aaaagattaa aaatggtaaa atgtatttgt
     2701 ttgataaagg tggtcatcct gcaattttat cgaatggatt agatttttca aatgtagcaa
     2761 agaaattttt agaggaataa tttaaccaaa atttataata atgaaaactg taaaatggat
     2821 tgcaaaactt acaaaagtga gtgtacatat acttcattat tatgatgaaa tagtattgtt
     2881 aaaacaaaat attctaatag tcaagttatt acatttgtat atgcaatatg tatagaaaga
     2941 gatatcatta gattgtaaag ttgggattaa ataacaatat taaattttat ttattcagat
     3001 attttagctt aactatataa atgttagaac aaatttatat agttaagctg taggattaaa
     3061 taatttagtt aaataagttt attatctcct catcagatag actttttaat acaccttcat
     3121 tagaaagttc tccatttata aattgattta taagctcttt tttactttct tgtaatttta
     3181 ttattttttc ttctatagtc cctttagcaa tcaattttat aacttgtact gaattttttt
     3241 gaccaaatct atgagctcta tcactagctt gattttctac tgatggattc caccatgggt
     3301 caaagtgaat taccatgtca gcagatgtaa gatttaaacc agttccgcct gcttttaaag
     3361 atattaaaaa aactttttta tccatactat tattgaactc atctacaagt tcaagtcttt
     3421 ctttggcatt agttttacca tctatataat ggtatttaat tttatactta tcaagttcct
     3481 tacttatgtt cttaagcaca gaagtaaatt gtgaaaacaa taatattttg tggttttcgt
     3541 ttatactatc cctaagtatt tctaaacatg tttcaatttt tgaacttttt ttattgtaat
     3601 cttttactac tatacttggg tctaaacaaa gttgtcttag tttagttaaa taagaaaata
     3661 taactatttt atctttcttt agatttttat ctttcatttt atcttgtata tcttttgaat
     3721 atacactata tatctttttc tgctctttat tcaattcaac aaaaaagttt ttttcaatct
     3781 tatctggaag ttctttcata acttgttttt tacttcttct aagaataaaa ggttttatca
     3841 attttttcag gtttttaaca ttatcttctt tatttataaa caattcctga aactttgctt
     3901 tactatatag atagccaggc attataaaat caaatataga ccataattct agtagattat
     3961 tttccatagg tgtaccagtg agtgcaaatt tattttctgc atttatactt tttacagact
     4021 cagttactaa agctactgga ttttttatat tttgagcttc atctaatata caataatcaa
     4081 actttatttc actatatttt tctaaatcat ttctaagagt accatatgtt gtaagtatta
     4141 catcaaaatt ttctaattcc ataaaacatt tttctctatc tcttttattt ccatgtatca
     4201 ataaaacttt tatatctgga gcaaactttt caaattcatt tttccaatta taaatgagtg
     4261 atgtcggtgt aacaataaga ctttttttat tagataatga taataaaaat gcaatagtct
     4321 gtattgtttt accaagaccc atttcatcag ctaatatacc accaaattta taataatcta
     4381 aaactttaaa ccaattgagg ccatttattt gatagtcacg aaggtttgca tttaaatttg
     4441 cgggaatttc taaatttatg ctatcaaaat ttctaaattt ttcacatata ctgtctagtt
     4501 cttttttacc atctataaac tttaaatttt tactacgtat tacatcattt atatatagag
     4561 ctttattttt atgaatttta ttattttttg atttatccat tatatctaga ttttcaacta
     4621 attcaaaaaa atcttttgtt tctctttctg ataaatctat aaagtttcca tctttaagtt
     4681 tataaaaagt acgtttttct tgaaaagctt tcaaaatatc cttgtactct cttggattta
     4741 tatcagatat attaaaatta aattctaggt aattctggtt agtctgattg attgaagctt
     4801 gaattgatga agcgttatat atttttttca atttaaactt ctcagaataa tatatatctc
     4861 caaattcctt tagagattct aattgtaaat ctaaaaatgt aaataggtca tggtcagttc
     4921 ctttaaacac ataatgatta ttttcctttg taaattgtga agaagtaagt ttattttcaa
     4981 tagtagcttc cttttgtatg ttggggagca caaatttttt atcttcatca tcatatatat
     5041 actttacatc acaacatatg ttgccatcaa tcatatcaaa gtagaactca gatttaaaat
     5101 cttttgttat attatttttt atgttatcat ctaaatgaac ttcttgagat atagatttta
     5161 atctaggaac tagcaatcct aaaacatcct tagaatcctc tttatcaaat tttaactctt
     5221 tagtctttga taatatttga tatatttttg aataatcatg agctttttta tgtgataata
     5281 aatagatatt gccatcatat aaaagtacat catttttttt agtaagtgga acaggtagtg
     5341 aatctttaga atataagact attttatctt ctatattttt aaaatcaaac cttacaggta
     5401 gagtatcatt taatatcttt ggatgatatt cctctttaga aaaaataagg gtaaaatcct
     5461 tgtacttaat agcatttaaa aattgtttta aagagcttgc atttatctcc ataaattttt
     5521 ttgaatgtga tgaagaaaag ttgtatgaaa gatttgtccc atattcttct ataatatcta
     5581 ctaacttctc atcttcatca gcaaaataat gagtgttggg gttgtaagta aatttcttcc
     5641 caaaatctaa ttcatatcca ttaagttttg aatatgcaaa ttcagtaatg ttttttaaaa
     5701 tgtacatttt atcaattcct attttaaatt cagcaattgc tttatttgaa tccacattta
     5761 tcaatacatc taaattaacc ctttctcttt tagtattgtc taacaaagaa agtaattgct
     5821 tgcttaaatc taagctggtt ttagaatttg tattagaaag cttatcctta tctgcaccta
     5881 atatatctat tagaatagga ggaacatctt ttttattttt tatttgtttt aagcttttta
     5941 tcacaatagc aactatatgc ttacatataa aaatactttt gccctttgat tgacagtcag
     6001 cgcaactaca atcattttca attatcttaa agtgctttat atctatagtt attgaattaa
     6061 aataaaagct ataattatct tcagaattga ctccaccacg aaatgtcaac aggttatttt
     6121 gtaggtcatc aatatctatg tctatataat caactaattg attagtataa tattttacac
     6181 ctcttgacca tcttgatggt tctgtatttc ttcttattag tttatatatc atatctattt
     6241 tcatattatt caccttacct cactgtatat attataatct aaatgcattc aatttacact
     6301 ctaaatttaa gactaaaatt aaggcagata tgaatagctt ttatctatca caatatatgt
     6361 gtaaataaca atttactatt agatttttat tgaattatct tataaactat gaatgaaaaa
     6421 taattatata aatagattat taataaatat aaaatcttat tattaaaaat attcttagaa
     6481 attacactat ataaaatata aagtatgata tttgaataaa aagaatgtat aataagtata
     6541 ataatttgta tttattattg ggaaaggaaa atgttaatat ggatagttat agacaactta
     6601 aagctaaaat accctgtaag gaaacaggta tagaaataaa gaggacttta tgtgatattt
     6661 gttctccagg agtacattgt ggaattgatg catatgttaa agatggtaaa gtaataaaaa
     6721 tagaaggtac aaaagaacat ccaatgaata aagggttgtt atgtaccaaa ggtctttgca
     6781 atagagaata tatctataga gaagatagaa taaaaacacc tctaaaaaga attggaaaaa
     6841 gaggtgaagg aaaatttaaa ccaatatcat gggatgaagc ttatagagaa atagcagaaa
     6901 aattaaatag agtaaaatct gagtatggag caaagcatgt agcttttttt acgggttatg
     6961 gaaagtggta tagacctttt ttacaaaggt ttgcatcttc atttggttca cctaattatg
     7021 gtacagaatc tagttcatgt tcaacatcag cacagatggc tggaaatgtt gctactggaa
     7081 gaaactttac aatgcaagat atggagaatg caaaaactta tttagggtgg ggatttaatc
     7141 catactactc taaattttta aatagtgtga atctaataaa attaaaagaa aaaggtctaa
     7201 aatttataat tgtagaccct agaataacac cgacaactca aaaactagca gatttacatt
     7261 taagaattaa acctggaact gatggagctt tagctcttgg aatggcgaat ctatttatac
     7321 aaaaggattt aatagataga gaatacatag aaaagcatgt acatggattt aatgaatata
     7381 aagaatatgt aaaattattt gatttagata ctgtaagtag aattacagga gtttctaaag
     7441 ataatatagt aaaagctgtc gagatgttgt cacaaggtcc attttgtata aatgaaagtg
     7501 cagctccaat agttcaccat caaaatggat ttcaaaatta tagagcaatg atgtctttat
     7561 ccgctataac aggtaattat gatagaatag gtggaaacat accaaaacaa gattactttg
     7621 ctggtaagat aggtggattc caaacactag aaaaagagtt tgtaaaagaa gttctaccta
     7681 aaaatataaa tgaaagaatt ggtggagaaa gatttcctct atggaatgaa atggtgactc
     7741 aatttcaagc tatggattta cctaggcaaa tagaagaaaa gacaccatat gaaataagag
     7801 ctttgttttg tcatgggata aatgctagaa tgtttactaa ttcagggaga ctttttaaag
     7861 cgttgaatag tgtagatttt tttgtgaata tagatttatt tatgacagat acatcaaaat
     7921 atgcagatat acttttacct gcatgtacat catttgagag agaagaatgt aagagttatc
     7981 caggagggta tattacatat accaaaaaag ttattgataa attatacgat tcaaaatcag
     8041 atgtagaaat actttcagat ttagcaaatg ctatgaatat agatgatgat ttattaaaag
     8101 caggttatga agcaagtata agacatatgt ttaaaaatac atgtgtagat gtagataaac
     8161 ttaaagaaag tgatttacca ttaaaatgta aagattttaa gccatatata gtaggttcat
     8221 atacaagaga agggtatgat actagcactt ctaggtttga attaaaatct acaattattg
     8281 aaaaatataa agattttggg ctggatgcac taccaacata tagaggattt aatgataata
     8341 tagatgatga atatactcat ttattgacat caggagcaag aatttcaaat gcactacatt
     8401 caagacttca tgacatcaaa ctttcaagaa tattaaaaaa tgaagctagt tgtgatattt
     8461 ctagcttaga tgcaaaagaa ttgggtgtta tcagtggaga ttatataaaa ataactacta
     8521 agataggaag tttggatgta aaagtcaatg ttactgatag agtcttatat aagaatatac
     8581 atatgtatca tggatataga gaagctgata taaatagtat tatttgtgat aattttgacc
     8641 cttatagtgg attccctgcg tatagaagtt gtaagtgtaa gattgaaaaa atgaatagat
     8701 aaatggagag atataatgaa aagtttattt aaatttaata aagaattatg tacagcatgt
     8761 ggagcatgtt tgcttgcatg tatagaccaa aatgataata atatagacaa tggtgaactt
     8821 tttagacatg tatttacaat agaggaaggt gaaaatatag aatatttttc attagcatgt
     8881 atacattgta atgagccaaa atgtttaggt gtatgtccta aaaattgttt taaaaaagaa
     8941 gaaggattca ttgtattaga caatcaaaac tgtatagggt gtaaactttg tgaaaaagct
     9001 tgtgaatatg gagccttaat ttatggaaat gatggtaagg caaataagtg taatggttgt
     9061 attgagagat ataagtgtgg tatggattca ccttgtgtaa tggtatgtcc tacaggagca
     9121 ttaaaattta ataaataaat taaatataga gaatgactta tacttaggta taagatattt
     9181 ttacaaaaag ctgtattgag tttgttataa tgaaaactca atatagcttt tttatggata
     9241 tagttggata ctataatgtg attcttagta atagtttttt gctattggac aaagactgta
     9301 aaacatatta aacttatgtt gttcaaagtc gaagtataat ataataacta gaaaattggt
     9361 ttatagtgct ataaaagcaa atttagtaag gtataaaatc agaggcaatc ctttactata
     9421 atatcaatat ttgggagttc tgttattgca gagctattaa acaattataa tggtacaagt
     9481 gattcaacct ttgaaaatgt attatataaa aaattaaaat aagattaaaa aggagagaat
     9541 aaattatgaa aaaacttaaa ttaattatta tgtttgcctt ggtatcagca ctgttagtta
     9601 caggatgttc atcaggaaat aataaaaata aagaagaaca aaaagttata agagttgcaa
     9661 catcaggaac atattaccct tttgctttta aagataaaga taaacttaaa gggtttgaag
     9721 tagatttttg ggatgaattt gctaaacgaa ctaattgtaa agttgaatgg gtattaactg
     9781 attttagtgg attatttgga ttattagaag ctgataaagc agatgttgta tctgctcaac
     9841 ttactgcaac acctgaaaga gagaaaaaat atgctttttc agatgcttat aattatagtg
     9901 gaacaaacat tattgttaga gaagatgata ctagtataaa atcaatagat gatttagttg
     9961 gcaagaaagt aggtgtgggt actggagcag tagcaaatga aatattaaaa gaaaaatacc
    10021 caaatgatga aataaaaata gtaaattatt caagtgcgac tcttaaaggg aattatcaag
    10081 acctagaaat gggaagatta gatgcagtag ttgcgcagga tgttgaggct ttaatagcta
    10141 taaaagaaca aaaattaaat cttaaaatga tagaacctcc aattcaattt ggtgcctgtt
    10201 cttttgcgct tcaaaaaaat gaaaaaggtg aaaaatggac aaaagaaatt aacgaagtaa
    10261 ttgaagagat gtataaagat gggactttga ctaaaatatc tgaaaactgg ctaggaaaag
    10321 acattactaa ggagcctatt aatgagtaat tttaattttg aattttgtct aagtatattt
    10381 ccatttttga ttaaagcatt gggaataaca atactaatat ctattgttgc atttttttta
    10441 tcaatattat taggtgtaat acttgccttt ttcacacaga caaaatcaaa agttttacaa
    10501 tacataacaa gagtgtatgt ttcattcttt agaggaacac cacttttaat tcaaatattt
    10561 ttcttttatt ttggatttcc aatgatattt tcttttatga aagattgttc accttattta
    10621 gcaatagtaa tttgtttagg tgtaaattca tctgcatata tggctgaaat tatacgagca
    10681 tctattgaga gtgttgataa aggacaacag gaagcatgtt atgcttttgg gttaagtaaa
    10741 tatcagtcaa tggttaaaat aattctacct caagcagcta catctgcagt acctcctatt
    10801 gtaagctgta cactggacat aattaaaggt tcttctttag gtttaactat aggaatacaa
    10861 gatttaatgg gagtagctca agtagaggct gcttcaagtt ttaaatattt tgagatatat
    10921 atgataacga tattaattta ctgggcagta tctataatat tgagtaatat acaaagcaaa
    10981 gttgaaaaaa gaataagaag agcatattag atttcaatat ttaaaaatgg gatgatatag
    11041 atatattatt acttgtaaat aaatttttaa ttttagaaaa ataaacttag taatctaaat
    11101 tattgagaaa atatagtatc aaaatggact gtataatcaa gttaaaaaat agtttttcta
    11161 ttgaaaaaat aaataaaatt ctattgtaat ttaatatgcg ataaagtttt ataaaaaaca
    11221 acattaagct agggggactt attttatggc ttttaatatg aaatattttt tagaattatt
    11281 tccaacaata atgaaatcgt tcaatgtaac tgtgattttg gcaataagtt cattagtatt
    11341 ttcattaatt ataggtacaa tagtagcgtt atcatcttat tacaaagtga aagtattgaa
    11401 tcaaatatgt aaagtatatg tatctatttt tagaggtaca cctcttatgc cacagttatt
    11461 ctttttatat tttggactag catatatgag tgattttgtt aaaaatatgg acccagtatt
    11521 tgcaacatct atagtactta gtttaaatat gggagcttat atgtctgaaa cgataagggc
    11581 atctatactt tcaattgata caggtcagat agaagcagca tatgcaatgg gattaacaaa
    11641 tttacaaaca atgaaaagga tagtgatacc acaggccgta agagttgcac tgccatcatt
    11701 attcaataat tttatagatt taattaaagg gtcttctgta gcttttgttg ttggagtaaa
    11761 tgatataatg ggtgctgcca aatcacaagg agcgttgagt tttaagtttt ttgaagtgta
    11821 tggagcagtt atggtaattt actggtgtat aataactcct ttaggttggg ggcaaaaaaa
    11881 attagaaaag aaaataagca attattaagt tattaagaaa atacataaaa gtatttttat
    11941 ataaaaaatg gtgtgaggag aagagatatg ataaaaagaa atattataaa atgtttagtg
    12001 gcagttttat cgttaacgtt tgtaattact ggttgttcaa aaaaagagga agtaaagaat
    12061 gaaaaagtta taactgtagc ttcatctaat tcagctgacc catattctta tgttgataat
    12121 ggtgagcata agggttttga agttgacatg tggaaagaga ttggtaaaag aagtggatat
    12181 aagataaaaa tggaaccaac aggttttagt tctatatttg gtatgttaga ttctggtaaa
    12241 gttgatgtgg cagccaactt ctttggaatg tctaaagaaa gattagaaaa atacgatgct
    12301 tctatacctt atggttctga ttctgtgtgt atagctgtaa aagatggaaa taacgaaatt
    12361 aataagcttg aagatttaaa gggaaaagtt gtagcagttt cagaaggttc tcaaggtcaa
    12421 caggttgcaa ctagtgttaa taaagataat ttgtttcaag aaaaggttta cggtgatgga
    12481 actagtggtc ttcaagattt agatttggga agagttagtg catggattga tgcagagctt
    12541 actattgtgg gagacgctaa aaaagctaat atgaaagtaa gagtattaga taaaaaatta
    12601 tcagctacta atatagcata cttctttaag aaaaacgatg ataaatctaa aacgataaaa
    12661 gaagatgtaa acaaagcaat agaagaaatg cttgcagatg gaacagtgaa aaaaatatct
    12721 gaaaaatggt ttggaatgga tgtaagtgct gatatacaaa aataaatact ttaaaattaa
    12781 aatgtaaaac tgtgataact atagataagt atactaaatt tatctatagt tatctgtgta
    12841 ttacaatgta tttagattag tctttataaa tcaaaatgag ttatggaggt attgcaaatg
    12901 attgaaacta aaataaaatc cattgcacta aaaaatagag aaaaaattat aaaaataaga
    12961 cgacagatac acagtaatcc agagttagca tttaaagaat ataaaacatc taaacttata
    13021 aaagaagagc taaataaact taatatagaa tacataaata ttgctggtac aggagtatta
    13081 gctactataa aaggaaaaaa taatggtgga aaaactatat tactcagagc ggatatggat
    13141 gctcttccaa tcaaagagga aaatgattta gaatttaaat caataaatga taatatgcat
    13201 gcttgtgggc atgatgcaca tgtttcatgg ttgttaggaa cagcaatgat tttaaatgac
    13261 ataaaagaag aattgaatgg aaatgtaaaa ttactatttc aacctggcga agaaaaaggt
    13321 ggttctgata ttgtaattaa agaaaatgta cttgaaggtg tggatgcatt agcgactggt
    13381 cattgttggc caactattga gtcaggaaaa attggaatcg ctagaaattg tgctatggca
    13441 gcgactaata cttttgaaat aacaataatt ggcaagggtg ggcatggagc agagcctcat
    13501 aattgtatag acccaattgc agttggaaat gcagtatatt catccattca acagatagtt
    13561 tcaagaaaaa ttgacccagt tgtacctgta gtagtgtctg tttgttcttt taattctggt
    13621 gtttctaaaa atataatacc agatgtatgt acacttcaag ggactattag agcgataagc
    13681 aaggaacaag taattgaagt aagtaaaatt ctagaatata tagtaaaaaa tgtatgtaaa
    13741 gcacatggag cagattgcaa atttgaaaag agtacaggag gagatgctgt tataaatgac
    13801 aaaaacatga tagagatagg aagagaaagt gcttgcaaaa tacttggagc tgaaaatgtt
    13861 gaaataattg attttcctgc aatgacaggt gaggattttg ctatttatat gaaagaaaaa
    13921 cctggtttat ttatgtatat aggtgtagga gataaagaaa aaaatataaa ttacagattg
    13981 catagtaaca agtttaatat agatgaaaaa tgtcttagta tagcaccttc attattttca
    14041 caacttacag tagattattt atgtcaacta tagagtacat tcataaaaat atatttagcc
    14101 tatttttaaa tttaatgcta gtatattaat tttgatgtag aacatattat ttattatatt
    14161 tatttaaaaa ggtggataac aatgataaaa gtagaaaatt tacataaaag ttttaaaggg
    14221 acagaagttc ttaaaggtat aaatttagag ataagtcaaa gtgaagtagt agctatactt
    14281 ggatcatctg gaacaggtaa atctacgtta cttagatgtt taaattattt agttgagcct
    14341 acaaaaggaa agataatgat tggtgatgta aaagtagatg tagaaaaagc aacaaaaaaa
    14401 gatataacca atttaagaaa acatacatct atggtgtttc aaaattataa cttatttaaa
    14461 aataaaacag cattagaaaa tgtaatggag gcattgatag tagttcataa aaagagtaag
    14521 aaagaagcta gagaaatatc tttaaaatta atagataaag taggtatgtt ggataggaaa
    14581 gatttttatc catcaaagtt gtctggggga cagcaacaaa ggataggtat agcgagagcg
    14641 atggcagtaa atccaaatgt aatattattt gatgagccta catctgcact tgacccagaa
    14701 cttgttggag aagttttaag cgttataaaa tctcttgcaa aagaaggtat aacgatgctt
    14761 attgtaactc acgaaataaa ttttgcaaaa gaagttgctg ataaaattat atttatggat
    14821 aatggtgtaa tagctgaaga aggaactcct gatgaaatat ttaataatcc taaaaatgaa
    14881 agaacagcaa aatttttaaa tatcattagg aaagaagaag tatttagtta aaagtgtata
    14941 aattaagcat tagaagatat gtaagatacc cccattaaat ttataagtta gataataaat
    15001 ttaatgggag tatattttta ttattagatt tattatttaa tttaaaatta gaaatgtaac
    15061 tatttgaaaa ttctttttaa aagtatatca aaatatcaaa tacataaaaa aataatattt
    15121 gacaaagtat catatgtg
//
LOCUS       DAEMGG010000097        15084 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEMGG010000097 DAEMGG010000000
VERSION     DAEMGG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.097"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1354
                     /locus_tag="KPK24_002735"
     CDS             <1..1354
                     /locus_tag="KPK24_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2300388.1"
                     /translation="IIIYCNKAFEENYGLKKEDILGKNVSFLEDSGYSTKSPIPIVLK
                     TKTKFSLEQDTQTGKKLIITATPIFDENGNLEFTVENCRDITELNNIKNKLEDTKKQV
                     KKYKSEVETLYRTALRIEDTVIMDGIVMRPIINTVNHVSKTDVSVLLLGESGTGKSSL
                     ARYIHHNSNRSNGPFITINCATISPQLLESELFGYTSGAFTGASTKGKVGLVELANGG
                     TLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGSLKNKKVDIRIISATNVDLVSKVKEKK
                     FREDLYYRLNVIEIKLPPLRERRDNLVEIIKYYFNRYSSDFNLNKTISKEAMDAIANY
                     RFPGNIRELQNIIQKILLTCTDNHITIDDLPNILTKNIHITNNGNKTHISQINKVIIS
                     DSKSTNYKNKNFDTLIKEYEKNIILDAYEKFGSSYKVAKHLEISQSKANRLIRKYTNT
                     "
     gene            1554..2138
                     /locus_tag="KPK24_002736"
     CDS             1554..2138
                     /locus_tag="KPK24_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG2300389.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(2242..3021)
                     /locus_tag="KPK24_002737"
     CDS             complement(2242..3021)
                     /locus_tag="KPK24_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2300390.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(3079..4335)
                     /locus_tag="KPK24_002738"
     CDS             complement(3079..4335)
                     /locus_tag="KPK24_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG2300391.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            4593..5492
                     /locus_tag="KPK24_002739"
     CDS             4593..5492
                     /locus_tag="KPK24_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG2300392.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            5516..6964
                     /gene="ddlR"
                     /locus_tag="KPK24_002740"
     CDS             5516..6964
                     /gene="ddlR"
                     /locus_tag="KPK24_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG2300393.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDIEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            7023..7829
                     /locus_tag="KPK24_002741"
     CDS             7023..7829
                     /locus_tag="KPK24_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2300394.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            7855..9774
                     /locus_tag="KPK24_002742"
     CDS             7855..9774
                     /locus_tag="KPK24_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2300395.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(10016..10363)
                     /locus_tag="KPK24_002743"
     CDS             complement(10016..10363)
                     /locus_tag="KPK24_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG2300396.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(10350..12278)
                     /locus_tag="KPK24_002744"
     CDS             complement(10350..12278)
                     /locus_tag="KPK24_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG2300397.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(12689..14878)
                     /locus_tag="KPK24_002745"
     CDS             complement(12689..14878)
                     /locus_tag="KPK24_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG2300398.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         5572 a         2279 c         1950 g         5283 t
ORIGIN      
        1 aataataata tattgtaata aagcatttga agaaaattat ggtctaaaaa aagaagatat
       61 tttaggaaaa aatgtttctt ttttagaaga ctctggatat tcaacaaaaa gccccatacc
      121 aattgtatta aaaacaaaga ctaaattttc actggaacaa gatactcaaa cagggaaaaa
      181 gcttatcata actgctaccc ctatatttga tgaaaatgga aacttagaat ttactgttga
      241 aaattgtaga gatataactg aacttaataa tataaaaaat aaacttgaag acacaaaaaa
      301 gcaagtaaaa aaatataaat ctgaagtaga aacactatac agaactgcac ttagaattga
      361 ggatactgta attatggacg gaatagttat gagaccaatc ataaacacag taaatcatgt
      421 ttctaaaacg gatgtaagtg tacttttatt gggtgaatct ggaacaggaa aaagctcact
      481 tgctagatat atacaccaca acagcaatag atctaatggc ccatttataa caataaattg
      541 tgcaactata tctccacaac ttcttgaatc tgaacttttt ggatatactt caggtgcttt
      601 tacaggtgca agtactaaag gtaaagttgg gctcgtagaa ttagctaatg gaggaactct
      661 ttttttagat gaaataggag atataccaca aaatctacag gcaaaatttt tgcaattaat
      721 acaggataga acttttactc cagttgggag cttaaaaaat aaaaaggtcg atataagaat
      781 tatatctgct acaaatgttg atttagtttc aaaagtaaaa gaaaaaaagt ttagagaaga
      841 tttatactat agattaaatg ttatagaaat aaaattgcct cctcttagag aaagaaggga
      901 taatctagtg gaaattataa aatattattt taataggtac tcaagtgact tcaatctaaa
      961 taaaactatt tctaaagaag ctatggatgc tattgcaaat tatagattcc caggcaatat
     1021 aagagaatta caaaatataa ttcaaaaaat cttgcttact tgtacagata atcatataac
     1081 aatagatgac ttaccaaaca ttcttacaaa aaatatacat attaccaata atggaaataa
     1141 aacacatata tcccaaatta ataaggttat tatatctgac tcaaaatcta ctaattataa
     1201 aaataaaaat tttgacactt taattaaaga gtatgaaaaa aatataattt tggatgctta
     1261 tgaaaaattt ggtagttctt ataaagtagc caaacacctt gaaatctcac agtctaaagc
     1321 caataggtta attagaaagt atactaatac ttaattttaa atttgataaa taaaaactta
     1381 acactattta catattgtaa atttggccat ttactgttat tatgaatatg gttgaaatta
     1441 tttaaataaa tactgttgac ttagtttcat atatttacca ttaaactttt atcataaata
     1501 tcatatcata ataatatatt taataagtct aataaactat acgaggtgta aatatgttaa
     1561 tgaataccca ttttattatg gcaaaatcaa tacttgataa tatagatgaa aataaaacat
     1621 tctttataag cgaaaaaaat tttatatatg gcaatataaa gcctgatgct ttttctaaat
     1681 ataaattaaa aaaacattat ttagatgagt catttaatat gattgtttct aaaataaact
     1741 atctatgcag tcttaatata gatagtcttt ctaaatactt ttcagtaagt agacttagtc
     1801 aggaactagg agtcatatgt cattttttat gtgatttttt ctgtgtagct cacagtgaac
     1861 gttgggagtt taagcatagc ttaaataaac atgttactta tgaaagagaa cttggagcat
     1921 ttgcaaaaga tgttgattta tctaaaatta aatgtggtac tataaatagt agttttgaga
     1981 agttttttac taaactttat agcgattaca aaaagaaaac tgattatatg aatgatttaa
     2041 aattttcagc ttatacttgt aatagtgtca taaattatat acttgactct attttatcta
     2101 atacaataaa atcatatcat atagcaaatt gcggttaact taagaatttt taaacttata
     2161 taaacttata attgaatata attaaaatga ctgctctata gaatataatt ttatacttct
     2221 ctaggacagt cattttttat actaactatt tatctccatt ataacatcta tggcactttt
     2281 tacactatct actacataat ttgcactttg ctttacatct tcccttgcct tagacattgc
     2341 aaaactatat ttaaatcctt ctaacatgca aatatcattt ggagaatccc caatagtagc
     2401 aacttcatct ggacttacat ttaacgtttt tattacatgc tctatacttc ctctttttga
     2461 cacactttta ggaactatat ttaaagtata tgttccagaa aaatatattt ctaagacttc
     2521 actaaactca ctttttagat aaagttctat tctggataac tcttccaaat tttcagaaca
     2581 tacgcctata tttcctataa gattatcaaa actacttctt ttttcaaagt ctgggtcaac
     2641 tataaagtca ctagaataat gtctaaaagc agtggtatca ctacccacaa ttacatcacc
     2701 cttatattta aaataaagtt gtgcatcact tgactcaaat ctatcaatga ctttttttac
     2761 taaattatca tctatcgttc ttttaaaaat tacattataa tctttatcat atataaatga
     2821 accattttca catacatagt atccattcat atctaaacta tctttgataa tattatgtgc
     2881 ttgtttaaaa attctacctg tagcaacatt aaactgtact cccttttttt ctatttcttc
     2941 aattgctact atatcttctt ttgttatggt tccattttca tatagagttc catctaaatc
     3001 acaaaatata tgttttatca taaattaaaa gcctccaaaa acaattttat attttactaa
     3061 tactttaatc aaaataactt aaagcttttc taattgtatt ctcaactcat taagcaaatc
     3121 tctagtagct cttaatctta tctttcttct atccccatta aatatatttt tctttactat
     3181 tgtttttcca tttatatata ttgcagtata aaccagtccc acaggcttct ctttggttcc
     3241 tccaccaggt ccagctactc cagttgtaga tatacctata ttagctttaa gtcctttagc
     3301 aacgccctca gccatttcta tagctgtttg ctcacttact gctccaaact cttccaaagt
     3361 ttctcttttt actcctaatc ttttcatttt agaatcatta gaatatgtta cacaaccttc
     3421 aagaaataca gaggatatac ctggatagtt tataagagat gaagaaacca aacctccagt
     3481 acaagattca gccactgcta ttgttagatt tttttctaca agtagcttag aaacagcatc
     3541 ttctaaagca gtctcccctt cagatatgga aatatctcct tcagtatata catatttacc
     3601 aactctactt tttatttttt cttctactgg ttttattaat ttaaaagctt ctctttcatt
     3661 ttcagccttt gcagttattc ttattgtaac ttctaattct tttgcatata aagcaactgt
     3721 tggattagtc tgctcattaa ttatatcgag aatttctaat tctagatttg actcacctat
     3781 tccatataat cttaaagtct tagatactag catttcattt gtgaattgtt gtaagtaagg
     3841 tttgacactc tcattaaaca tagctttcat ttctcttgga ggtccaggca atactattat
     3901 aaatttacca tcttttttta aaattgcccc tggagctgta ccattattat tttttagtac
     3961 tatagcatct tttggaaagt acacttgttt catattattt tcggccattt ttttccctgt
     4021 tttaacaaag aaagattcca aaacctctag tgaaggttta tgtagttcta atttctgacc
     4081 aaagtattct gcagcagttt cttttgtcat atcatcacca gttggcccaa gaccacctgt
     4141 agtaattaca aaatcacttc tctttaagct ttcatcaaaa cattctaaaa gtctttgttt
     4201 gttatctcca acagtactct gatggtatac ttctattccc aatgatgcaa gttctttagc
     4261 taaaaattga gaatttgtat ttactatatc tcccaataat atttctgttc ctacacttat
     4321 tatttcagct ttcaaaattt atcactcctt gatttatagc cattaagact agtcttaagt
     4381 tcataacaat aattttagca aaaataataa cataaataaa gttaaaaaat catttaaaca
     4441 tatctttaaa ctagattatt attctaaaac cacacataat ataaacttcg tatatagtgg
     4501 tactttcatc ctcaactgtt aattcatata attaagtatg taaataaaaa gtttacttta
     4561 aaataataaa aattaaaata ggagtgtata atatgaaaat agcagttata atgggtggaa
     4621 tatcatcaga gagagaagtt tctttaaatt caggaaaaga gatatataat aaccttgata
     4681 aaaataaata tgaagttgta aaagtaataa tagatgataa aaaagatata tttacaaaga
     4741 taccagaaga tatagatttt gcaatacttg ctttacatgg aaaatttggt gaagatggat
     4801 gtatacaatc aatacttgag actatggata taccatactc tggatgtgga cctctttgca
     4861 gtgggatgtg tatggataaa aatataacta aaaaaatgct tagagattca aacctaccta
     4921 ctgctccatg ggttttagta aaatcagttg atgaaataga ctatgatgaa atagataata
     4981 taggttatcc agtatttata aaaccaaact caggaggttc aagtgtagcc acattcttta
     5041 ttcattctaa ggatgaagtt gaagaagcag ttagaaaagg tctggaagtt gatgaatttg
     5101 taatgattga aaaatatata ccaggtggag aatatacttc attcatatta aatggagaag
     5161 tgttccctac aatatctata aaatctgatt caggattctt tgattatgaa gctaagtact
     5221 cagttgaaaa aggtgcaaag gaagaagttg tttacttaga tgaagaactt caaaaaagag
     5281 taaacgaaat ttctgagact tgttggaaaa tttttaattg caaagcatat gtaagagttg
     5341 atatgattat aagtgaaggt attccatatg ttcttgaact aaatactctt cctggaatga
     5401 ctcaaactag tttaattcct agaagtgctg ctgctagagg tattaagtac agtgaactat
     5461 tagataaact tatagaatac tctttaaatt agatgaaata atttgaggtg gtaatttgat
     5521 attttcaaat ctaaaactaa atgataatga accaatttat atacaactta aaaattatat
     5581 aagtgatatg atatcaaaag gactaattcc agacaatagt aagctccctt ctacaaggga
     5641 gcttagtcag cttttacaag ttagccgaaa ttctgtagtt ttaacttatg aagaattaaa
     5701 atcagaagga ttaatctact ctatttcagg aaaaggtaca tttgttaaat caaagaataa
     5761 gtcttcaaat actacatggt cattaaactg ggattgttta gagaatacat actctaaaaa
     5821 agccaatgaa ttggacatta taaaaagtga aattccttgg agctctgacc tgatttcctt
     5881 taaaagtatt tctccagatg gagatttatt cgacatagaa gaattaaaaa aatccttttt
     5941 aaatagaatt tcactagaag gacataaatt actcaactat ggatatgcac aaggttataa
     6001 gccccttatt gactatttac tagaatatat gacaaataag ggtgctgata taagtaataa
     6061 agatatacta ataacaaatg gattcacaga aggctttgat attattatgt catcttttac
     6121 ccaagaaaaa gactacataa tatgtgaaaa tcctactcat aatactgcta taaaaattat
     6181 gaaatctcat aacctcaata tcttaggtgt agatataaat gaagatggtt tggacttcaa
     6241 tatgctagaa gacaatttag ttaagtataa aaataaaata aagtttgcat acattacacc
     6301 ctcttatcac aatcctacag gaattgtaat gactcctgaa aataggtaca aattctataa
     6361 tcttatgaaa aaatacaata tagcaattat agaagatgga tttaatgaag aattactcta
     6421 caatagttct catatatttc ctatctgttc cctagataat atgaataatg gggtcgtata
     6481 tattggtagt ttttcaaaaa ttttatttcc tggaatgaga attggatgga tatttgcaga
     6541 taaaaaagta atcaataagc tagaaagtgt aaaaaggtgc agaaacatac atgtatcctt
     6601 tttggaccaa ggaattttat ttgattattt aagtaatgga ggttttgaaa aatacattaa
     6661 aaaaatacgt aaattctatg gggacaaatt taactttgca tataagtgta taaaaaaata
     6721 tataaaaaca gaatatatct tgggtgatgg tggtttgcat atatttataa aactaaaaaa
     6781 tatagatact agagttttat tagaaaaatg ttatgaaaaa aatgttatat ttatgcctgg
     6841 agatttattt tttacagata actctggttt tgatacttta agactcggat tttctaggtt
     6901 gtcttttgaa gacattgaaa ttggaataaa aataattgga gaaactataa atgaaatgtt
     6961 ctaggttaac taaatattgt tagcctagtt tatttttatg aggtataata tatatagttt
     7021 atatgaatct aatactgggg gataatatga gtaaaaagtc aaaacgaaga aaaattaata
     7081 aattaaaatt aacgattgta ataactgtat tatttcttgc tttattatct atatatgaga
     7141 tactgttctc taatcccaac aaaaaaggta atataaagag tcaagaaact atgcaaaata
     7201 aaaataatac aacagatgat tctaaaaata atcaaagctc taccaaaaag caggaagata
     7261 ataatatcaa gcctactttt tcatattctt caataccaga taatataaaa aataaaatga
     7321 ttggtaaatc tatgcctact gatgaaccta taagctttga tagtctatct tatttaaaac
     7381 ttacatatta tggatttgat gaaaaaactc atcaaggaga aatgatagta aacagtgaac
     7441 tagctccaga agtagtggat atttttaagg aattatacga aaaaaaatat ccaattgaaa
     7501 aaattaagtt aatagatgac tatgatgctg tagatgaaaa atctatgtct gataataata
     7561 cctcatcatt ttgttataga actatagctg gtacaaatgt agtttcaaat catggtaaag
     7621 gacgtgctat agatataaac ccattacaga atcctcaagt ttctggaaat gatgttacac
     7681 ctaaagtaag tacagtttat gctgataggt ctagcacaaa atttggaatg ataaaaaaag
     7741 gtgatgattg ttataatgct tttgtaagca gaggttggag ttggggtggc tattggaaaa
     7801 acccagatta tcaacatttt gaaaaataat agattagaaa taaaggtgaa ttatatgaat
     7861 ttaatgacat tggaaaatat aagtaaaagc tattctgaaa aaaaattact agaaaatata
     7921 tctcttggaa taaatgaagg ggaaaaaata ggacttattg gtgtaaatgg tactggtaaa
     7981 tctaccttac tcaaaattat tgcaggagtt gaagaaagtg agactggtaa tataacaaaa
     8041 actaatggta ttaggataga atatctacct caaaatccaa cttacaatga aaattcaact
     8101 gtacttgaac aagtatttaa tggaacttct gaagaattaa agctacttgg tgaatatcaa
     8161 gaaatcctag aaaaacttaa ttcaaacttt acagaggaat taaataaaaa attattatca
     8221 gtacacgaaa aaatagatgc cttaaatctt tgggatttag aaagtgaagc taagtcagta
     8281 cttacaaagc ttggtataaa tgactttaat caaaaaatag cagaactttc tggtgggcaa
     8341 agaaagagag tctctctggc atcagccctt attacacctt gtgagttttt gatactagat
     8401 gagcctacaa accatctaga caatgatact atagattggc ttgaggaata tcttaattcc
     8461 agaaaaggct cattgcttat gataactcat gatagatact ttttagaccg tgttacaaat
     8521 agaattattg aactggacaa aggtagatta ttcagttatg atgggaatta ttcaatattt
     8581 ttggaaaaga aaatggaaag actttcgcta gaagcaagta ttgaagataa aagacaaaat
     8641 ttaataagaa cagagcttgc gtgggtaaga cgaggagctc aggctcgtag cactaaacaa
     8701 aaagctcgtt tacaaagatt tgatgaactt ataaatagag attcttttaa acctgatgaa
     8761 aatcttgata tatctgttgc atcaagtaga cttggaaata aaataataga aatacacaat
     8821 atatctaaat catttgaaga aaatactgtt atagataatt tagaatacac tttaactcgt
     8881 acagatagaa ttggtataat aggcaaaaat ggtatgggta aatctaccct tataaatata
     8941 cttaatggag aaatagagcc tgatagtgga tatatttcaa ttggagaaac ggttaaaata
     9001 ggttgttttt ctcaagacga ctctcatatg aatattgata tgagggctat agactatgta
     9061 aaagaagcta gtgactatat agaaacttca gatggtacta ggattacagc ttctcaaatg
     9121 tgtgaaagat tccttttcaa tggtactatg caatatactt tgataggtaa attatctggt
     9181 ggtgaaagaa gaagactaca tcttcttaaa atccttatga cagctcctaa tgtgcttctt
     9241 ttagatgaac ctacaaatga tttagatatt gaaacgctta aaatattaga agaataccta
     9301 gacgaattta aaggtgtagt agttacagta tcacatgata gatatttctt agatagaatt
     9361 tgtaataaaa tatttgcata tgaaggtaat ggaaaaattc atatctatac aggtaattac
     9421 agtgattatc ttatttatag agagattcaa ggaattgaat ttgaagaatc taaaaaggaa
     9481 tcagttaaaa aagatgactc tactacccta aaaaaagaaa aacctaaaaa tgataaaaag
     9541 cttaagttct catataatga acaaagggag tttgaaaata ttgactctga tatagaaaaa
     9601 ctcgaatcta aaattgcaac acttgatgaa agtactgcaa aattttcaac tgattttact
     9661 aaacttcaag aaattttaga tgagaaatct aaactagaaa aagaacttga atacaaatat
     9721 gaaagatggg aatatttaaa taatctagct gatgaaatag ctaataataa gtaatattaa
     9781 aataatctta agtttaataa gttttgcttt aaatctgtaa tttataaata ttttaaagga
     9841 agtgtctcaa aatgaacttt ttagttttat gagacactct tttttatgag aaatcaaata
     9901 tattttcatc atttcaacaa tacaaaagag gcttatctct attttgagac agcctctttc
     9961 ataattacct tctaatttat atgctctaat atatctataa taattcttat tatatctata
    10021 tatccagtct ttcaatataa tgacaattaa cttcaatatg atttaaaaat actaacaacc
    10081 tttcataaag tggatgagat tcttctccat atttagcttc taggttcttg cctatttctc
    10141 taacagtcct ttgaccatca atttgtaaaa aaacaaaact ggaatattca tctagttcta
    10201 tttttttgta tttaggtatt ctagctttta actttctaaa aaacttttga attttatggt
    10261 cctgtttttc aagtatagta acaactctat tttcagctac ttcatattca agtccatcag
    10321 taattttaaa tacaatatct aaaacatcat tactcttgct cattattttt tactttagat
    10381 aaaacaatag gtaatgctgt aagcacaact aaaactacta ataaagcaat tgccattgaa
    10441 tttgttgcag caaatccact tggtaccttt ggagttacaa ctccagttac ttgtaatatt
    10501 atacctatta aaccaatgat agaaccacca gcaacaagac cagaagataa acttatacca
    10561 ttagaaactt taacttcttt ttctttttca gttttagata ctaattcaac aaataaacga
    10621 attaatgctc caactaatat tatagaagtt gttgcaattg gtaaatagaa tcctatagct
    10681 atagtcatta ttggtaaatt taatgcatat agaactattg ccataaatac tccaacaata
    10741 atcataaccc aaggtaaact acctgacata ataccagaag ttaatgttga cattaagttt
    10801 gcttgtggta atgcaaattg tacattatca ccagttgctc taagttggtc agaaagtaat
    10861 aaaattactc ctacaacaac tataacacca actatacttg caattgtaaa gtaacgttgc
    10921 atttcgtttt tgctaccacc aataatataa gttacttttt gagattgagt ataacctcca
    10981 gctatagcaa tagcaacaac tataaatgaa ccaaataaaa gtaatgattt attagcttct
    11041 aaatcagtcc atcccattat aacaaacact aatgttacaa taactagaga agctatagtc
    11101 ataccagaaa ctggaagatt tgatgttccg attgttcctg ttaaacgacc agctactatg
    11161 acaaataata aagataataa aagagatatg atagctccaa taattgccat aacaatgtta
    11221 ccagaaatta agaatgctgc taaaaatcca ataactactc cacctaataa aagtatcatt
    11281 tgtatagatg aaccttcttc tccttctcca gcacttgatt tagctttaag tgtttctttt
    11341 atagaagcga ttatagttgg tataagtttt attgcaccaa taatacctcc acaaagcatc
    11401 ataccagcac ctatatattt tacataacta ctagatattg ctccaacatc catttggtta
    11461 agaggcattg caggattatt ccataccata gctccatctt tagccatatc tgtaaagtat
    11521 cctattaaag gtgctatacc aaagtttgat aaaatacttc ctgcaaacat tgttaaagaa
    11581 acttcaagcc ctactataaa tccaattcct aaaagtagag gatttacttc aatatcaaat
    11641 ttccatttgt agaatttgct tcctacaaga ctcattacat tgtttgctac atttaagaat
    11701 gaaccagtta atacagtaat aaatccactt attacaaatc caattcccat gtacttaatt
    11761 gaatctccac cagcctctga agcaacaagt gtttcagata tagccattga ttctgggtac
    11821 ataagtttac catgttcttc aactattaga tagttgtgaa ctatagcagc aattcctata
    11881 ccaaataaaa ctccaccaac tccaacagct aagccttcaa agaatgtaat ttggcttcca
    11941 attaatataa ctgctggtaa tacgaaaata aatccacttg caacagattc tccaccactt
    12001 gacatacctt ggattaagtt tttaccaaga ataccctttg aacgagcaaa tgctccaaca
    12061 aaagcagaac ctattatagc tcctggaata cctgcggcaa ctgtaagacc ggctttcatt
    12121 ccagagtaag ttgtagatgc ggcaaaaatg gcagctaaaa taataccgat aatcagtaca
    12181 gctacatttc caccggttct ggacttatct gtgatatatg gaacgtaatc ttttccacta
    12241 acctcaccat aagcaccctt aggtaatttt ttgttcataa aattttcacc ccttttcaat
    12301 ttgtatatat tataacatat atacatataa aacttaactt tttattattt tacaaatagc
    12361 gagtcaaaac tactaaaatt taaccctata ttattaattt ctacttttag atatttttat
    12421 gctaattttc tttttatttt tcaattctac taaaaactat taagatataa aataaaaaaa
    12481 ttttttaatg taaaattaat tttgtaaaaa aaatgcttta aaacacggaa aagtataaaa
    12541 aatacaataa tcattcttat aaataaattt tcaccatagt aaatatatac agaaaattct
    12601 tgtttttatg caaattgatt cttttataaa aataagtgtc ataaagaaat gtctgaaatc
    12661 actttatgac acctatcata ataaaaaatt attctggtaa tgctctttta tttacttttc
    12721 cgattggtgt aactggtatc ttatctatcc tcattattat tgttggaatc atatatggag
    12781 gtagatgctc atttaaatat tctctaatag ttctttcatt tatcttacca ctatcttcct
    12841 catcaattac ataatatgca catatataca tatctccatc atcatttgtt ctcgatatac
    12901 aaacaacact tttaatttct gtaatattat ttattatatt ttcaatctca tatagctcta
    12961 tcctaaactc atcaattttt acctgattgt ccatccttcc aatataatct aaatttccat
    13021 catctaacca tcttactaaa tcaccagttc tatacattaa cttattatgt tcacaagttt
    13081 tgtaaggatt ctctacaaat acttgctctg ttagcccttt tttatttaaa tatcctcttg
    13141 atatttgaat gcctgataga catagctctc ctggtgctcc aattggacat agtttgttct
    13201 ttttatctaa tacatatgca taagtattaa atataggttt tcctattggt atttttccat
    13261 attctctgtc cacatgaaaa gaagtcgata caacagtaaa ttcggttggt ccatactcat
    13321 tatatagttc ataattacgc tttctatatc cttttaatct ttctcctccg gtctgtacaa
    13381 ctctaagtga attattattg caattttgta taaataacct tgcaaatgct gttggcaatg
    13441 ctagtatagt tatgtcattt tcctcacagt attcatttac tgtatattca tcaaatttag
    13501 cttcttcagg cataatatct attgatgcac ctacaagcaa ataagggaat agttgtttca
    13561 ttacagcatc aaaactgaaa cttaagtata taccacatat atcattttct gtagcgctta
    13621 tagattttac gctataataa cacatattta ctaaactctt atgttctata gctatggtct
    13681 ttggcctacc agttgaccct gaagtagata tcgtatatgc acaatttgat gatgttattt
    13741 tttcagttaa ttcatatcca agcttaaaca tttcttcatc atttatatcg agtactatgg
    13801 tattctcatt tacaacctta cttttgagtt catctgtagt tatcactact tttgcattac
    13861 tatcttcaag aaggtaattc aatctttctt ctggattact tgtgtctatt ggaaaaaatg
    13921 ctccccctga tttcataatc cctattgctg taataattat gttaatatct cttggtaaca
    13981 ttacagctac tatgtcttca ctttccactc ctatatgatt taaataacct gcaagtttat
    14041 cagttacttc atccaattct ttatatgtta actttctatt ttcatcccta gcagctatat
    14101 tactaggtgt taccttaact tgctccctaa ataaatcaac ataactttta tcatatggta
    14161 tttcatattg attattatta aagtcttcca ataatacttt ttcttcttcc ttacatacaa
    14221 attctaaatc ttctattaaa atatcattat cttctattat ttgctctatt atataatcaa
    14281 attgatttaa aattcttcta atagtttctc ttttatatat agatggttta tactttattt
    14341 ttattttaag tgccctctta gtatatacta ttacagataa atcatatcct tttttttcta
    14401 tgtcataatc atatgataaa tcaaaacttt tagctataaa tattgtgttt ataagcttag
    14461 tatttagatg atttttattc tctatatctg ataacaaata gtatccatac tctaaagacc
    14521 tgctccactg cctttttagc ttccttacaa catctataaa tttttcactt tgtctaactc
    14581 ttactcttat tggaattata ttcattgttt taccaaaaac tacatcatct tcatatatat
    14641 acttttgaag tataaggcca tatatagttt ctatgataga gtctatgcta atattgttca
    14701 ctttagaata ttgaattaac ctttcatgtt tctcaatatt catcttaaaa acctcatcta
    14761 ctacttcatc agaacaatct ttataaaaaa aatcatttgg aatttttgct atattcgtat
    14821 aatcatctaa caaatccatc caataattca tagctcgttc taaattaact tcatacatat
    14881 caaaattccc ccttactact aagtctatag aaaaaattct tttggaattt tattttcaca
    14941 atatataaat atattaataa tacatatgca agctctaaaa acatgagctt gcataaaatt
    15001 acacttatta tgactactaa attcgtaata tttacaatgt ttaaaatata ttataacaca
    15061 tatatgattt tatttttcta catt
//
LOCUS       DAEMGG010000098        15073 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEMGG010000098 DAEMGG010000000
VERSION     DAEMGG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.098"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            186..764
                     /locus_tag="KPK24_002746"
     CDS             186..764
                     /locus_tag="KPK24_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2300399.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            803..1381
                     /locus_tag="KPK24_002747"
     CDS             803..1381
                     /locus_tag="KPK24_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2300400.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1467..2096
                     /locus_tag="KPK24_002748"
     CDS             1467..2096
                     /locus_tag="KPK24_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2300401.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2086..4845
                     /locus_tag="KPK24_002749"
     CDS             2086..4845
                     /locus_tag="KPK24_002749"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG2300402.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            4932..6494
                     /locus_tag="KPK24_002750"
     CDS             4932..6494
                     /locus_tag="KPK24_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG2300403.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6495..7631
                     /locus_tag="KPK24_002751"
     CDS             6495..7631
                     /locus_tag="KPK24_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG2300404.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7695..8771
                     /locus_tag="KPK24_002752"
     CDS             7695..8771
                     /locus_tag="KPK24_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG2300405.1"
                     /translation="MKYFDYICKDDLEKIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNRHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8749..9987
                     /locus_tag="KPK24_002753"
     CDS             8749..9987
                     /locus_tag="KPK24_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBG2300406.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10041..11129
                     /locus_tag="KPK24_002754"
     CDS             10041..11129
                     /locus_tag="KPK24_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG2300407.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLISKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11157..11948
                     /locus_tag="KPK24_002755"
     CDS             11157..11948
                     /locus_tag="KPK24_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG2300408.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12063..12617)
                     /locus_tag="KPK24_002756"
     CDS             complement(12063..12617)
                     /locus_tag="KPK24_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG2300409.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTHKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12813..14015
                     /locus_tag="KPK24_002757"
     CDS             12813..14015
                     /locus_tag="KPK24_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG2300410.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14072..15052
                     /locus_tag="KPK24_002758"
     CDS             14072..15052
                     /locus_tag="KPK24_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG2300411.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
BASE COUNT         5942 a         1562 c         2739 g         4830 t
ORIGIN      
        1 catttaataa agtatgtaaa atctatgtaa aaagtaagaa atataaacat ttttttaatt
       61 atattttaaa aatatcaagc aagttttctt cacaaatgaa taaaaaatgt tataatatga
      121 ttaaagaaaa agataaataa ttagcaagat ttgtttagct aaaattaaca agggagcgat
      181 aaattatggg tataacatta tcaaaaggcc aaaaggttga tttaactaaa ggaaatccag
      241 gccttaaaaa tattttagta ggtttaggat gggatactaa caagtatgat ggtggatttg
      301 actttgattt agatacagca gcatttttaa caggagcaag tggaagtgtt actaatgatg
      361 gagattttgt attttacaac aatttaaaac atacatctgg agcagttgag catctaggag
      421 ataacagaac tggcgaaggt gatggagatg atgaacaaat agttgttgat ttatctaaga
      481 ttcctggaga aataagcaag atttctttta ctgttacaat tcatgatgca acagaaagaa
      541 gacaaaattt tggacaagta agtaattctt atataagaat agtagataaa gacacaaatg
      601 aagagttaat aaagtatgaa ttaggagaag attttagtat agaaacagct atagtagtag
      661 ctgaaatata taagcacaat ggagaatgga agtttaatgc actaggttct ggctttgaag
      721 gtggtcttgc tgcattatgt ggaaactttg gaataaactt ataatttcaa ctataacaat
      781 aaactaaagg gggatatttt ccatggctat agtattaaaa aaaggacaaa aaatcgattt
      841 aactaaggga aatccaggtc ttaaaaatat aaaacttgga ctaggatggg atacaaattc
      901 atttgatagt ggctatgatt atgatttaga tgtaagtgtt ttcatggttg gagaatctca
      961 aagagttgaa aaagatgaag attttatatt ctacaataac ttaaaacatc catctggagc
     1021 agttgaacac ttaggtgata atagaactgg tgaaggtgat ggagatgatg aggaaatatt
     1081 agtgaattta gagacaatgc caaagcatat acaaaagata gctgtaacag ttacaatata
     1141 tgaggctgct gaaagaagac aaaattttgg acaagtaagt aactcatata taagagtatt
     1201 aaattctgaa aatgatgaag aaatattaag atatgactta ggtgaagaat ttagtataga
     1261 aactgcaatt acagtatgtg agatatataa gtataatgga gaatggaagt ttagtgcagt
     1321 tggagcagga tttgaaggtg gcttagaagc actttgtaga aactatggct taaatgttta
     1381 atttaagcaa tataatatat taaatataaa taacctatac aaataatgta taggttatat
     1441 tatataaaat tttgtggagg tttagtatgt cgattaattt atcaaaaggt gataaaatag
     1501 atttaaaaaa atctaaccca ggactgagta acatacttgt aggtctagga tgggaccctg
     1561 tgcaacaatc tggcggaggt ttttttaaat cgttatttgg tggaggacaa gctgatatag
     1621 attgtgatgc atctgtattt atgcttaatc aagaaggtaa attaagtgga ataaaagatt
     1681 taatttattt tggaaatcta aaaagtgcct gtaaatcagt tttgcataca ggagacaatc
     1741 taactggtga gggtacaggt gatgatgaac aaatcctagt taatttggat aaagttccat
     1801 caaatattca taaattattg tttgtagtaa atatatacaa ttgtgtagat agaaaacaac
     1861 actttggaat gatagaaaat gcctatataa gagttgaaga ccaaggtaat aagaaagaga
     1921 tagcaaaata caatctatct gataattatt ctgaaaaaac tactcttata gtaggagcta
     1981 tatatagaaa agatggaagt tggcaattta aagcaattgg tgaaggaaca aaagatgcgg
     2041 gtcttaaaga agtaatgcaa aatttagaca ggattgagtg tgcatatgga atttaataat
     2101 gatatcaaat attataatgt agataaagaa gaagttacaa aagacttatc tgttaatcct
     2161 gaaaaaggtt tatcagaatc agaagtcaag actagaagag aaaaatatgg tttgaatgaa
     2221 tttactccaa aagaagaggg tagtttttgg gatgatttaa aggaaagttt aagtgaacca
     2281 atgatagtta tcttgattat tgcagcagta gtaagtgcag taattgggga aactcatgat
     2341 gcaataggga ttgtaggtgc tatagcaata ggtatagcaa taggtatgat tacagaaggt
     2401 cgttctaaga aagcagcaga ggctctttct aagttgactg aaaacataga agtaaaagta
     2461 ttgagagatg gggaagtaca tcaaatttct aagagtgaat tggtaccagg tgatatagta
     2521 tatattgaaa ctggtgacat ggtacctgct gatggtagac ttatagaaag tataaacctt
     2581 aaaattagag aagatatgtt aacaggagaa tctgatgacg tatctaaaaa gtgtgatgta
     2641 gtagtttcta tggaagagat agaatctaaa ggagcagtag tagtacagga gccaatacct
     2701 gcaaaacaga taaatatggt gtttggtggc acacttgtag cttatggtag aggagctcta
     2761 gttgtaactt caattggaga taatagtgaa atgggtaaaa ttgctcaaaa cttatcagaa
     2821 acagatgagc aaactccact tcagataaaa cttggaaatt taggtggttt gatagccaaa
     2881 gtttctagtg cagtagcagg attactattt atatttatgg ttttccaaat gataagtaag
     2941 aatgtattaa atgtagatat gtctggaata ttaccgtttt tagaatcaat agacccagtt
     3001 aaaacagcat ttacagtatg tgttgcgttg atagttgcag cagtgccaga aggtcttcca
     3061 actatgataa atatgacact tgcaataaca atgcaaaaaa tggctaaaat aaatgcattg
     3121 gttactaaaa aagaagcttg tgaaacgata ggttctgttt ctgtaatatg ttcagataaa
     3181 actggtacat taactgaaaa tagaatgact gtagaagttg catatgttga tggcagatat
     3241 attgaaagtt cagaagagga aataaatagt tattttgaag aaaactgtat gataaactct
     3301 acagctgatg tagagcacaa tgatggtgat attaagtatt taggaagtgc tactgaatgt
     3361 gcattgcttt tatattacaa aaacgtagat tatagacaag ttagaaaaaa ttctaatata
     3421 gtagctcaaa atccttttac ttcagattca aagagaatga cttctgttat aggtcaagat
     3481 aatcatcatg tactattatc aaaaggagca ccagaagtct tacttgaatt atgttcacat
     3541 attcaaagag gaaaagacat agttccatta actgaaaata taaaacatga gattttagaa
     3601 gaaataaaga aattgcaaat aaaatcaatg agaactttag ggtttgcata taaagaaatc
     3661 tcacaagctg aagaagaagc agcagtaact gctgaaagtg atgctatgaa tgtaagtgca
     3721 atggaaaaca atttagtatt tagtggtttt gttggtataa gagacccact tagaaaagat
     3781 gttatagagt ctgtaaatac agcaaataaa gctggtgttt ctgtaaaaat gcttacagga
     3841 gataatataa atacggcaag agctataggt gaagagttag gcctattaaa aaataatatg
     3901 agagtggttg aagcttcata tatagatact ttagatgatg aagaattaaa acaagagata
     3961 caaagtattt caatcgtggc aagaagtaaa ccagatagta aaatgagaat agtttcagct
     4021 ctgcaaaaga gcggagatgt tgttgcagta acaggtgatg gtataaatga tgctccagca
     4081 ctttcaaaag cagatgttgg tatagcaatg ggtatatctg gtacagaggt atctaaaaac
     4141 gcagcagaca taattttaac tgatgatagt tttagtacca ttgtaaaagg tattaaatgg
     4201 ggtagaggaa tttatgataa cttccaaaga ttcgttcaat tccaattaac tgtaaatgta
     4261 attgcatttt tagtggctat aatatctcaa gttatgggac aagagatgcc atttactaca
     4321 atacaattat tatgggtaaa catcattatg gatggaccac cagcactagc attaggttta
     4381 gagccagtta gggaccatgt gttaaataga aaacctgttg atagaaaagc caatataatt
     4441 tctaagtcaa tggtgtatac aatagtatta aatgcatttt atataacagc aatactaatg
     4501 ttacaatcta catttaattt cttaggagtt gatgaagcgc ataagggaac agtaatgttt
     4561 gcgttatttg cttttagtgc actgtttaat gcatttaact gtagagagtt taatgcagat
     4621 agtatcttcc ctaattttac acataacaaa ttagcactac aaataatagt gttgacagga
     4681 attgcacaag taatatttac tcagctattc caagatttct ttaactctgt agcaatggat
     4741 tttatgacat gggtaaaagt tttatgtgta gcagcaagtg taattgtggt taatgaagtt
     4801 gtaaaatgta taataagact ggtaaaacca aaaaagactg cttaaaacaa attttttaga
     4861 agaaaacaga ctgactcaaa atatgaattt tgggtcagtt ctgaataaac atttaaattg
     4921 ggaggatagt tatgggtgac tcaaaggtat ttgaaaaaat attctccagc caatcagata
     4981 gaggaaatta cactccttca aagggatatc taagttattt tatttcgtat attggtttag
     5041 aagatgaagt tttgtacaat ctcgagatat ttaagacaaa acaaaatatt gatagcaaaa
     5101 aagatattgc tttatttact gatgttatag caaatcctag tgattttgat attataaatt
     5161 attttaaaag tggacttcaa aaatatagaa ctagcatgga agatgtagat ataaatattt
     5221 tgggatttga agagattgat tataaaataa agcaagcaat ggatagagtg ttaaaagaag
     5281 aggaaaaaga atttactaat gatagagtta aacaaaattt tatagtgaaa ataatggcat
     5341 ggataaaaat ttatatcggt gctttagata taaataaaaa tgaagctcca aaagtaatat
     5401 tttatggaga tataaaaaaa catgaagttt atttactctt aatattatac ttggcaggtt
     5461 ttgatgtttt atatcttaat ccaaattcaa agagtaatat agatatttta aaatcagaaa
     5521 gatacaatat agagtttgaa gaagccaaca taatagaaga aaaaatttct tttgaagaaa
     5581 gggttatttt aggggaaaaa atagataaaa gttctgtaaa aaaagctttt actgttgggg
     5641 cagaagcatc aaagcgtata agtgaggaat tgttaaatga tgcaggattt ataaaacctt
     5701 ggcaactcca agatagaaaa ataaaaaatc tacttctaag ttcaactgtt gatgaaatat
     5761 caatatactg gaatcaacca cttaaattaa gaccaggatt taagtttaat gatgctattg
     5821 tagaagctcc aaattttctt tctaagatta atggaatata taacgataag aatgaatata
     5881 ttaagttttt agatttatta agagattcgg aaagttcaac atttattgaa tttaatggag
     5941 atgtagatag attttcaaaa gcttttacaa gagaagcttt tagtttatcc tttttattgg
     6001 attcaaaagg tgcaatagat aaaaactctg ttttaaataa taaagattat tccatatcaa
     6061 cactagcatt gaatcaacaa attatgattt tagagaaagt ggaagagttg ttagaaggaa
     6121 gtatgttttt gaatggttta agtggagaag acaagataaa aggtctattt acagtacttc
     6181 acatggataa gaaatttgtt catatgatga acaatttcga ttactcactt ataaacccaa
     6241 aattgattat atacatgtac aagagtattg tatttgataa agaaatagtt tttcttatgc
     6301 ttttacttag taagattgga tttgatatta tcatattatg tccaggagga gaaaacaata
     6361 tagagaatgt tataaataac caattgattg atgtacacag attggataaa atggtatatg
     6421 accttaaatt aaattcgctt gaaaatgata tacctttact taaaaaaatt tttggaaaga
     6481 ggagacgatt ttagatgggg ataaatataa atagtttttc aaaagataag aactctgtag
     6541 aggttactga gaatttgcct acagcagttc aagaagaaaa cttcgatata atggaatata
     6601 caaataataa aaaaaatgaa ctgagaaagt ctaaggaagt tgaggcattg acatccctta
     6661 tagaagtaga aaatccagat acgattttac agtttggtag gaaagcatct gaaggagtag
     6721 caagagtttc tgatagcctt ttaaatacaa taaaattaaa tagaaatgaa gaaaattcta
     6781 aaatgctggt tcatttaact aaaataatgg acaagtttga tttagacgat tttcaagaaa
     6841 ctaaggaacc aaatttcgtt caaaaacttt tcaaaaaagc taataatgcc attgaaatga
     6901 tgtttcaaaa gtatgaaaca ttgggaggag aagttgagaa aattcaaatt gaacttgaac
     6961 aatacgaaag agatatagca ctttctaata agcaaatagg tgctatgtta aatgaaaact
     7021 ttgagtttta caatgaactt caaaaataca ttgttgctgg agaaatggca atagaagaaa
     7081 tggataatga aatactacct ttctttaaac aaaaatcaga gactagtgga gaccaaatgg
     7141 atgttgtcaa ttatcaagaa ctattaaaag tttatgatat gttaaatcaa agagtctatg
     7201 atttaaggat agcagaaaat atagctatcc aaacaatacc tatgcttaga ggtatgcaac
     7261 ataataatta tggtttaatt agaaaaataa atagtgcatt tgtcgtaact ttaccagtat
     7321 ttaaacaatg tttatctcaa gctatattac ttaaaaagca agaattacaa gcaaaatctc
     7381 taaaagcact tgatgacaaa actaatgaat tattgcttag aaatgcacaa aatgtaagta
     7441 ctcaaagtgc acaaatagct agaatggctg gaacaagttc tgttcaaata gaaacattgg
     7501 aaaaaatgta caatactatc aaatctggta tagatgaaac tatgagaata gaagaaaaca
     7561 atagagcttt aattaaggat aatacaaaga gattggaaga attaaatacg actataattt
     7621 ataataagta gtattatatt aagggctaat ttgtataaag ttggcccttt ctatgtatat
     7681 gaggaggcta gacattgaaa tatttcgatt atatctgcaa agatgatttg gagaaaattt
     7741 ttttgaagga gccagaagat tttagtgcta agactgaaaa agatgtgttg aaatatgcac
     7801 tgggcgcttt tttatacgtt ccagctactc aatataatat gatttataag agtattatag
     7861 gggatgttaa gggagttaga ccattagcta tttgtttaga agatgctgtt ggagtaaatg
     7921 gagagttgga ggctatagaa aatcttagat taattttaaa aaatattagc aatgaaagta
     7981 taactaataa agatggaata ccactaattt ttgtaagaat aaaagatgtg gagcaactgt
     8041 tgagaattaa agagattata attaaaaata gacattctat aacaggaata ttaattccta
     8101 aagcaaattc ggagttaata gaaaattgta tagaagcttt agattctatg aatcttcaag
     8161 atatgtatgt tattccgata atagagacaa aagaatttat atataatgaa aaaaaagaac
     8221 ttagttttac taatttgtac aatgctattt taagacataa gtcaagaata ttaagcatta
     8281 gagtaggcat aactgatata ttagggatgt atggtataag gagagataag aacttctcta
     8341 tatataataa tttgatttgt tcttcattta tattagatat aatcacatat cttcaaagac
     8401 cagagttaga tatacctata agtggtggag tatcagaatt ttttgatatg acgaataagg
     8461 atataagaaa taagtatata gaagaaatat tattggataa atatcatgga ttaattggaa
     8521 agaccgtaat tcatccaatg caaattcaaa ttgtacaagc gctatctaca gtatcatatg
     8581 aagattttac tgatgctttg gatatactgg atagtacaga cagtaagtat ggtgtgagca
     8641 agggtgtatt aggagagaga atgaatgaaa caaatccaca ttttttatgg gctaaaaaaa
     8701 cattgattct atctaaaata tatggtgtat taaataaagg agtagactat gaagaattgc
     8761 ttaaatttta attgtgagat tgacattatt gataatccat tgaatttaga actaaatgat
     8821 tttttagacg taggaataag aaataatagc aaaagacgtt ttttgtttat ctcaaagaaa
     8881 ctcggtaaac atttagcatg taagccaagt gagatggata atttgggaaa attaatggct
     8941 acaatttata atgaaaaaaa taaggaagaa tcaaatggta ctgttatagc ttttgctgaa
     9001 actggtacag ctgttgggca tagttttttt gattacttat gtggagatta tgaatttatt
     9061 catactacta gagagcagtt tgacgagtta gaaaatctgg aatttttaga ggaacattcc
     9121 catgcaacag accagaattt atactttggt atgcttaaca actttaaatt tggagatgag
     9181 ataatattgg ttgatgatga gattaccaca gccaatacat gcatgaatat aattaggaaa
     9241 attcaaagca tttatcctaa aaagaaatac actatatgct caatactcaa ttgggtaagt
     9301 gatgaaaatt taagtaaagt tactcgttta gaaagagagc tagattgtaa gatagatttt
     9361 gtatacttat ttaaaggaaa ctttaaattt tatatagatg aagatattat ttcaaattac
     9421 aacgataatt atagtattac taaattagag actatttcaa gtgataaagt aaatttagta
     9481 gtaaatcata tttatgttga tttagaagat tatattggaa ataagaaata tgtaaaatat
     9541 acaggaagat ttggaattaa tagaagagaa caatttagat taaaagaaat cgttaagaga
     9601 gaaagtaaaa aaataactat ggccagtaaa gaatctttaa aaaatgaaaa agaaaagatt
     9661 ctatttttag gtactgaaga atttatgtat atacctatgc tgtttgcaaa agaaaaagaa
     9721 aatcaatatg atatatacta tcattcaaca acaagaagtc caattattaa tattgataag
     9781 aataattatc ctattaaaag taaatttatg ctaaagagtt tttataatat agatattcaa
     9841 aattatattt acaatataga taaacataat ttttctaagt gttatttatt tgtagagctg
     9901 aataaaagtg aaaattctta tctagaattt attgatataa ttaaaaaaac aagtataaaa
     9961 gaattaacta tagtatgttg tagctaaagt gttttaacag gataaaatat atcacgattt
    10021 taagatagga gttgagtcat atgagagatt ttagcacgta ttctaaagat gatgttattt
    10081 ttttattaaa agatatatca aatttaattg aagaagaagg aaataaagaa agagaattaa
    10141 aaatacaaaa tggtagacat tattcagaaa tgataccaat agagtatgaa gttagtgaag
    10201 attatctgaa tttatatcat gagaaactaa gagaaaataa agataaactt gcattttcta
    10261 taggagttat gtgtgaaaaa ataattaaaa aaaatggaaa tgatgtagta ctagtttcac
    10321 ttgctagagc aggaacacct attggtattt tggcaaaaag atatataaga agcaagtata
    10381 atgtaagttt acctcactat acaatctcta taataagaga taagggaata gatatgaatg
    10441 caattgaata tatagtaaag aatcatccga gctcaaaaat tcaattttta gatggatgga
    10501 ctggtaaagg aactatatca aaagaacttg aaaaagcatg tgatgaactt aatgttaaat
    10561 ttaaaaaata ttttgactca acacttgctg tattagctga ccctgctgga tattcaggac
    10621 tttatggaat tagagaagat ttcttaatac caagtgcatg tttaaattca acagtttctg
    10681 gtttagtaag tagaactgta ctaagagagg atttaattag taaaaatgac tttcatggag
    10741 ctaagtttta tagacattta aaagataaag atgaatctat gaactatatc gaaactatag
    10801 aagaatgttt taaaaatcaa tttaagaata tatctgatga agttgaaaac tgggaaagtg
    10861 acattataac aagagatgga tactttgatg ttttaaatat aaaagaaaag tataacataa
    10921 cagatattaa ctttatcaaa ccaggtgtag gggaaacgac tagggtttta cttagaagag
    10981 ttccgtataa gattcttgtt aaagacttaa atgataaatc tttagaccat atattcatat
    11041 tagctaagga gaaaaatgtt gaagttgaac aaatggattt aaaagcctat aaatgttgtg
    11101 gaataataaa aaatatgaaa gatatttagg aaatatatta gacaggagaa tattgaatga
    11161 ttgttttttc agatttagac aggagtatca tttactccaa taaattttta aatgctgata
    11221 gtaaatatgc taatatagaa atttataggg aaaaagaaat atcttatata tctttagata
    11281 ctataaattt aataaagcaa atacaatatt atggtatgtt tatacctaca actactagaa
    11341 cagtggagca atttaagcgt atagaattta ataaatatgg gatttatttt ccttggagta
    11401 taacttcaaa tggaggagtt atacttaaag ataatgaaat attgaaatca tggagtgaaa
    11461 aaatagataa actaaaatct gattatgagc ccattgaaag tatgattcat aaatttaaag
    11521 actatttgaa tgtagatgga ataactaatt ttaaagttgc agaagataca tttttttata
    11581 tagttgttga tttatcaaga tttaatttgg attcaatcaa agaatataca aatatattgg
    11641 aatcaaaaaa ttggaagttt tatgtgagtg gcagaaaaat atattttata ccaaaagaaa
    11701 tttcaaaaga aaatgctata aaatacttga ctaaagaatt aggaatagaa tatttttatg
    11761 cagttggaga ttctattatg gactatggta tgcttaatat ctcaaataaa tcctatgtat
    11821 tgaagcatgg tgatataaat aaaaatgaaa tagaaaattc atttatatca agttctttca
    11881 atggaatgag tggaactgaa gagatactat ctaacatttt agatgagaac tgcctactta
    11941 atatataatg actgatatat aaataataaa ctagaaaaat ctgaaataaa ttatagtaaa
    12001 gtttatcata ctaatttatt tcagattttt atcaatttat atttaagtaa gaactcaata
    12061 tgttaaaatg aaggtggagt cattgcccag actgagatac atacctcatc atcataattt
    12121 tcatacacat gtggtatagt actgttaaaa taaaaactat ctccttcctc caatagatat
    12181 tccttatttc caagtgttat cttcatttta ccttttatta tatagccaca ttcttctcct
    12241 tcgtgtgtca caaattcact gtttttgtgt gtatgaccat ctagtgtaac aagtataaat
    12301 tctatttttt tcccagataa attaggaaca agaagttcgt aaaattttgt agaatcatta
    12361 gttactattt tttttcgatt atttttcttt actactatat cagagtcttc tcttccaatc
    12421 tcttcaaaaa aataacctat atttatatca agagcttttg atattttcca cattgctaca
    12481 atagatggaa caaccatatt acgctcaatt tgacttatta agcctgtact taaatctgaa
    12541 ttttttgcta aagtagagat acttatattt ttagattttc taagagatct aattttgctt
    12601 ccaacatcta tattcattag atacaaaccc cctcagacat gattataaca tataatttat
    12661 aaagattaat aaaataaaaa aaaagtttta caatacaagt attttttatg ctatattgaa
    12721 ttttctgaaa agatgcatta taatagtatt aatattcaat atattgaata ttgataacga
    12781 tattttaatt taatgaaatg gaggtaatta agatgaagag ttttgtaagt cctgataata
    12841 tttgggtatt atgggcagtt gtaactggat gggcagcatt tagtatttat ttggaacaaa
    12901 agtacaattg ggcatcaaaa gtgtcgggag caataatagc tcttgtagga gcaatgttat
    12961 tatcaaatct aaatattata ccagtagagt ctgttgtata tgaccaagta tggggatatg
    13021 ttgttccttt agccatagct ttattgttat atcaatgtaa cattaaaaag atatggaaag
    13081 aaagtggaag attgctcatt atatttcttg taggttctgt aggaactgta cttggagcta
    13141 tgattggatt tttagcatta aaaaatgtag taccagattt aaatattgtt gcagctatga
    13201 tgacaggttc ttatataggt ggaaatgtaa actttgctgc tatgtcaggg gcgtttgatg
    13261 cgccaggaga attagtatca gcaacagttg tagcagataa tcttttaatg gcattatatt
    13321 tctttgtctt aatagctata ccatcaattg gattttttag aaaacatttt aaacatccac
    13381 atgtagatga aatggaaagt ataggtataa ctgaaggaga aacagtagca gcaaattatt
    13441 ggggaagaaa agaaatatct ttaaaagata tagcaatagc cattggtagc gcttttataa
    13501 ttgtcgcagt atctgtagaa ctatcaactt ggtttaaagc tataatacca ttatcaaatc
    13561 cttttttagc aatgttaaat actttatttg gaaatcaata tctaataata actactttga
    13621 ctatgttgtt tgccacattt atgcctaatt tctttggtga tataaaagga gcacaggaat
    13681 tgggtacatt tttaatatat atattctttg ttgtagtagg tgttcctgct tcaataacat
    13741 taataattca aaaatcacca ttactacttt tatactgcgg aataatggta ctaataaaca
    13801 tgctagttac atttatagtt gcaaaaatat ttaagtttag tttagaagaa gctattcttg
    13861 catctaatgc aaatattggt ggtcctacaa cagcagcagc tatggcaata tcaaaaggat
    13921 ggtctaagct agtagggcca atacttatag ttggaacatt tggatacata gtcggtaatt
    13981 attttggtct tttagtagga aatattttga tataatttta cgtgaaaaaa agttgttttt
    14041 tagaaagatt tttatttaat tggaggttgt tatgattttc gatggacatt cagatacttg
    14101 ggcagatgta acaataagaa ggcttaacgg ggaaaaagat gtatttagaa gaatccatct
    14161 tgataatttt aaaagagcaa atatagaagg agctattttt gtaatatgga tagacccacc
    14221 atatgataaa gagcctgaaa aaagagttaa acaaattata agctctataa aagatgaaat
    14281 tgaagatatg aaagatgttg taaacatagt aaaaaaatat tcagatatag ataaaggtat
    14341 aagtgaaaac aaaataaata ttttaattgg tcttgaagga ttaagccata ttggaagaaa
    14401 tatagattta ctagattatt tttataaaga agttggtgca agacatgcat cattaacttg
    14461 gaatgaacag aatgaacttg ctacaggatg tttaggggat gaaaatcgtg gtttaactga
    14521 aataggtaaa aaagcagtca ataaaatgga aaaattgggc atgatagtag atgtgtcaca
    14581 tttaaatgaa aaatcatttt gggatttaat gtctgttgca agtaagccag ttatagcttc
    14641 tcattctaat gctaggagtt tatgtgatgc taaaagaaat cttacagata atcaattaaa
    14701 agaaatttca aatacaggtg gattagttgg aataaatagt tttagaggat ttactaatag
    14761 taatcctaag aagcaagatt taatacattt ggttgaccat atagactata tggtaaatct
    14821 tataggtata gaacatgtat cttttggttt tgattttagt gattacttag ggaaagagac
    14881 tataggttct tttactggaa atgctagtgt gccaagtgta gatggtcttg aaagcacaca
    14941 tacagcacag aacatagtag tagagcttga aaatagaggt tataaaaaga aagatataga
    15001 gttaatctct agtggtaatt tttataggct ctttaaagat atactaaaat aatataataa
    15061 agaacttaaa ttt
//
LOCUS       DAEMGG010000099        14877 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEMGG010000099 DAEMGG010000000
VERSION     DAEMGG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.099"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..409
                     /locus_tag="KPK24_002759"
     CDS             <1..409
                     /locus_tag="KPK24_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG2300412.1"
                     /translation="NLNMNPKTININFDILQKLINLNLIENFSEEGVFIECKKSQLII
                     NITKDKKIRETYLLSKEGKSYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVN
                     PLRLKDKVKVENNFDADLEDNRINYISNIGMII"
     gene            410..853
                     /locus_tag="KPK24_002760"
     CDS             410..853
                     /locus_tag="KPK24_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300413.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSRHTLPTTDKTREKFSKIKLNEIKKIYETIGINIITKISIDK
                     NKLEIEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            868..1452
                     /locus_tag="KPK24_002761"
     CDS             868..1452
                     /locus_tag="KPK24_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300414.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANLLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            1705..2742
                     /locus_tag="KPK24_002762"
     CDS             1705..2742
                     /locus_tag="KPK24_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG2300415.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            2823..3188
                     /locus_tag="KPK24_002763"
     CDS             2823..3188
                     /locus_tag="KPK24_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300416.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            3193..3510
                     /locus_tag="KPK24_002764"
     CDS             3193..3510
                     /locus_tag="KPK24_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300417.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            3558..5162
                     /locus_tag="KPK24_002765"
     CDS             3558..5162
                     /locus_tag="KPK24_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300418.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            5286..6914
                     /locus_tag="KPK24_002766"
     CDS             5286..6914
                     /locus_tag="KPK24_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG2300419.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTSLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAALDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNIYFSEEGQSELD
                     VMYNRVVSAYTYALESMRSDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            7179..8528
                     /locus_tag="KPK24_002767"
     CDS             7179..8528
                     /locus_tag="KPK24_002767"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG2300420.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8684..9763
                     /locus_tag="KPK24_002768"
     CDS             8684..9763
                     /locus_tag="KPK24_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG2300421.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            10108..11016
                     /locus_tag="KPK24_002769"
     CDS             10108..11016
                     /locus_tag="KPK24_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG2300422.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            11021..13390
                     /locus_tag="KPK24_002770"
     CDS             11021..13390
                     /locus_tag="KPK24_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG2300423.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKQDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            13715..14518
                     /gene="proC"
                     /locus_tag="KPK24_002771"
     CDS             13715..14518
                     /gene="proC"
                     /locus_tag="KPK24_002771"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG2300424.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         6126 a         1500 c         2703 g         4548 t
ORIGIN      
        1 aaatctaaat atgaatccaa agactataaa tataaatttt gatatattac aaaagcttat
       61 aaacttaaat ttaatagaga atttttcaga agaaggggta tttatagaat gtaaaaaaag
      121 tcagttaatt ataaatataa caaaagataa aaaaataaga gaaacatact tgctatcaaa
      181 agagggaaaa tcatatgagt tcataaaaag attattaagg gattttaagt atgtttatta
      241 ctatggtata gaaaattgtt ttattgaaga atactttaaa tatacagata aatctattga
      301 agtaaatcca ttaagattaa aagataaagt aaaagtagaa aataattttg atgcagattt
      361 agaagacaat aggataaatt atataagtaa tataggaatg attatttaag tgaaagtgga
      421 taaaacgata aattttctta atggatttaa aaaaactaaa ccatgtaata aaatctataa
      481 aataataata gtcttaattt taacgtgtat tttatttaaa tcatttatgt atgtgaaatc
      541 tataaatgaa cttgaagatt atataagtca aaataagcta gtggataaat caagacacac
      601 tttaccaaca acagataaaa ctagagagaa attttctaaa ataaaactta atgaaataaa
      661 aaaaatatat gaaacaattg gaataaatat tattactaaa atatcgatag ataaaaataa
      721 gctagagatt gaaggtgtgt gtagtgattt agatgtttta aacaatctaa ggaaaataaa
      781 gtacataaaa tattattaca tagacagtat tgtaagagat ggagaatatt atagatttag
      841 aataaaaagt tagatgggtg attatttttg agattaaata atagagaaaa aaatctactt
      901 aaaatattgt taatattagt tattatattg gcaagtgata tagcagtaag ttatttggaa
      961 gaaaacttaa ctagtttgaa gaatgaaaag caaaatataa taaataaaat aaataatcaa
     1021 gataattatc aaataaagga taataagcat atagatgata aatatataga aagtgttgaa
     1081 tcaaataaaa atgaagaatt aaaaaataat tttaataact catttttaga taaaaaaaca
     1141 aataaagata tagtgttatt agtagaaaat gaactgagag atattgtgaa tgtagaatat
     1201 attaatcaag aaagtagttt agataatcac aataaaaaac aagttaattt agaaataaaa
     1261 gttaatggag aacttgcaaa tttgttgaaa cttgaaggtg taatgaagaa gttaagacta
     1321 aataataaac ttgaaaagat agaaattaat aaattaaaag tcaataattt tgatgttcaa
     1381 aatattccaa atgaatataa acaagaaaaa gacaatattg ttgaatgtaa aatgacagtt
     1441 agagtcatat aacaaaaaag aatagataaa gtaaaaaact attatcaatt aataaaattt
     1501 tatagaggaa ttagttttac gataattcat ttctacaaga gggattgttg atgattttat
     1561 cgaaatccta attaaagtta atatattgta ccaaaatata agtgaattta atagaatatt
     1621 tacttatact atagattgat taataaaaaa atggtataat aacaaattgt actttaattt
     1681 aataataagg gagtggaagt catattgtta gggagaaatg agctatgccc ttgtggaagt
     1741 ggcaaaaaat acaagaaatg ttgtttaaat aaagatattg tttcagaaag agcatgcaga
     1801 aaaactgaac tttcacaaaa acaatataca gatttatatt caaagttata tgattattca
     1861 agacaggata agtttaaaga agaatgtgaa aaagctaaag aaatgtttta tataatgcaa
     1921 aatgatagtg taaatgaaaa atttgaaaga ttttttaata cttatttaat acaagaccac
     1981 ataatggaaa ataaaaaggt tatgacagtt ggttttcttg aagaaaatgg tgcaaaccta
     2041 agtcaatcag aagtatcaat attaaaaagt atgtttgaat catacgtaag tatttatgag
     2101 gtaaaagaag tttctgctgg aaaaatagta ttaaaggact gtttaagtgg aaatgaactt
     2161 tgtacagaag atgtaaaact tctgagaagt tttaaaatcg gaagttgtat gatagctaga
     2221 atagttgata ttgaaggaac taacatattg atagatataa ctataagtat atcaaatgag
     2281 gttaaagata taattttaaa ggatattatg aatctattca atcaatatca agatgtatac
     2341 aaagatatga agtcgttctt aatttatcat acacatatac tatacaagta tatacaacag
     2401 cttttagacc caagtatagc tgaatattta aagagagaaa gagataaaaa cgatacaaaa
     2461 gttaattcag aaaatgaatt agctgaagaa gaatgtaaag ttttagatac tcttaaagaa
     2521 aatgtagaaa aagaaaatat agatgcatgt atagagtttt ggaatgaata taagaaaaat
     2581 catgaaaata taaaaggagc tgaaaatggt tgggcagctg ctgttgaata ctatgttaaa
     2641 aaagaagcag gacaacctat aactcaagtt caaatatcaa agaaatacga tataagctct
     2701 agtacactag gaaagagata caaggattta aaaatatctt aattaattat aaaaagcttt
     2761 aagtcataga taaagaatct atagtataat atatgtataa tgtttagaaa gaggtattaa
     2821 taatgagtct tagtttagga aatgcaattg ctgtatcaac gaactttata aaatctaacc
     2881 cagctgtacc tgtgtacaat ggagaagaaa tatatgcagt tatttatcat atagaaatag
     2941 gatataataa tggtgtatca cctgatacca aacaatatat aataatagac tataaagagt
     3001 ttgatttatc tatggataaa aaaaatgaac taataaatat tgctaaagag tactgtaaat
     3061 ctaaaggtat tgtagaaaac gaaaaccttg aaataaacct tttagacaat gaagaagcag
     3121 agaacttctt agaaaaggtg tttaataata tgaaggttat aagaggtctt ataactaaaa
     3181 aaggataaat aaatggacta tataggaata gaaaatataa caccttatga aaatacatat
     3241 gaatttagtg tatatgaata tgatgatgaa atcaccttag gtagtgaaaa gttatatgta
     3301 tgtgaattaa gggttgtatt gattaaagtt aattctctgt atgttgaaag attgcataaa
     3361 tcagttgaag caatggtctt agtaaaaaac ttgaaaaaag atttagataa aacacttgtt
     3421 gtaaacaaaa taaagaattt tgtgctagat gagatttggg tagaaaatct agtaaaagag
     3481 aatatagaag ttatatttgt agaaagctag gaataaccct agctttttta tattacaatc
     3541 taagagagga aaacaatatg ttaagagtat ctaatgtaaa acttggaatt gataaagata
     3601 tatctttatt aaaagatgtt atacttaaga agctaaaaat aaaagaaaaa aacttagtca
     3661 aatatagtat atataaagaa tctatagatg ctagaaaaaa aggaaaaatg gaatttgttt
     3721 attctgtaga tgtagaagtt aaagatgaaa ataaaatact taactcaaaa ataaaagatg
     3781 taacaaaaat aaaggaaatc agatacgtaa atgtacctgt gggtgataaa aaattaaaga
     3841 ataatccttt ggtaattggt agtggaccag ctggtttgtt tgcaggtctg cttttagctc
     3901 aaatgggtta tgaaccaatc atacttgaaa gaggtcttga tgtagacagt agaaccaaag
     3961 atataagtga tttttggaca actagaaaat taaaaaataa ttcaaacgtt caatttggag
     4021 aaggtggagc aggaactttt tcggatggta aacttacaac tagaataaaa gatatacgat
     4081 gtagaaaagt attagaagag ttagttcagt ttggggcacc agatgaaata ttatactcac
     4141 ataagccaca tgttggaaca gatatattaa aaaatgtagt taaaaatata agaaatgaaa
     4201 taataagttt aggtggtcaa gttagattta attcaaaagt aacagatata gttgtatcaa
     4261 atgattctat acaatctgta ataataaatg atactgaaaa aatagataca gatacaatta
     4321 tattagctgt tggacatagt gcaagagata cttatgaaat gttgtattca agaggtataa
     4381 aaatagttca aaaacctttt gcaataggag ctagagttga acatccacaa gatttaataa
     4441 ataaagcaca gtacaaggat ttttataatc accctaggtt aggagctgca gattatagac
     4501 ttatagagca tgtttcaaat ggaagaacag catatacttt ttgtatgtgt ccaggaggta
     4561 gtgtcatagc atctgcatca aatgattttg aaatagttac aaatggtatg agtgaacatg
     4621 caagagataa aataaatgca aatagtgcat ttttagtaaa tgtaacgcca gaagatttta
     4681 agagtgataa tccactagca ggtgttcatt tccagcagaa gtatgaaaaa ttagcttttg
     4741 aattaggtgg aaaaaattat aatgcaccag ttcagcttgt tggagatttt ataaacaata
     4801 agacttcttt gaatttagga agtgttgagc catcatatag accaggatat acacttacaa
     4861 atttaagtga atgtttgcct agcttcgtaa cagagactat gagagaagcg tttatatctc
     4921 ttgataaaaa attgaatggg tttgcaatgt atgatgctgt tttgacggga gtagaaacta
     4981 ggtcatcatc accaataaga atagtgagag atgaaaatac tttagaatca gtgagtatta
     5041 agaatctata tccatgtggt gaaggtgctg gatatgcagg tggtatagtt acagcagcag
     5101 ttgatggtat aaaatgtgca gaaaaaataa tacaaaaata caccaataat acaaaaattt
     5161 aacataaact taatgatttt ttaatattta acatgaattt aacattatca ctcctataat
     5221 ttgataaaat acaataggtt ataaaatttt tagatacagt aatctcttta caaggaggaa
     5281 ataatttgaa cattataata agtctaatgg gtggattagg tctgttctta tatggtatga
     5341 accttatggg agaaggtctt caaaaatctg ctgggacaaa gctaaaaaag ataataaaac
     5401 tacttactag taacctattt atgggagttt tggtaggtac aggagttaca gcagtaatac
     5461 aaagttcaag tgctactact gtaatggttg ttggtttcgt aaatgcagga ataatgactt
     5521 taaaacaggc aataggagtt ataatgggtg ctaatatagg gactactgtt acagcccaat
     5581 tggtatcttt cgacttaact ggtatggccc cagtagcatt aggtgtaggt attatacttt
     5641 atttatttgg aaataagcct agaataaaaa atatagccga aatacttata ggttttggta
     5701 tattatttac aggtatggat tttatgaaga tggcagtgga gccactaaga gactatcaag
     5761 gatttactga cttacttgtt acatttggta gatatccact acttggatta ttattgggat
     5821 ttgggattac agcaataata caaagttcaa gtgcatctat gggtatgttg gttgcactgg
     5881 cagcagaagg tctagtgcca ctatcagcag cactgccaat tctttatggc caaaatattg
     5941 gtacatgtgt aacttcactg ttatcaagta ttggtgctaa caaaaatgca cgaagagcag
     6001 ctatgatgca tctaatcttc aatgttttag gaacggtgat attcttaata ttcttgaata
     6061 aaccagtagt aagcatggtt acaagttggg atccaagcaa tgttgcaaga caaatagcca
     6121 atacacatac attgtttaat ataatatctg tattgatact tttaccattt acaagtctta
     6181 ttataaaact agctataaag cttgttcctg atagagcagg tgatatagat gaagatgaaa
     6241 ctaaaactat taaatatata gatgatagaa tgatagaaac tccttctata gctcttgcaa
     6301 atacgataaa agaagcactt cgtatgggtg agaaggcgaa ggaaagccta aatgcatcta
     6361 tggaagcttt agtagaacat tcaactgaaa aaatagataa gacatataga cgagagagat
     6421 taatcaatga tttacagaaa gctattttaa attatttact taaactttca aaagcagctt
     6481 tagatgatga ttcaagagaa gttgtggata ctttgtttaa tacagtcaat gatatagaaa
     6541 gaattggtga ccacgctaaa aatatagctg aattatcaca agttgcaata gattcaaata
     6601 tttatttctc agaagaagga caatctgaac ttgatgttat gtataataga gttgtttcag
     6661 catatactta tgcattagaa tcaatgagga gcgataatgt tgatttagct tgcaaggtta
     6721 taaaaattga agaacaagtt gatataatgg agaaatcttg tagagctaat cacatgtata
     6781 gattgaataa taatctatgt agtatagaaa atggagttat ttatttagat gtaataagta
     6841 atttagagag aatatctgac catgctgtca atatagcaca acaagttata gcgaagagat
     6901 taggaaatga ttaaaaataa ttaatttaaa ataatataaa atttaaaata gaggataaat
     6961 aaaagagaaa ctttaaatga tacgtttaga gtttcttttt ttgtaaattt atttgactaa
     7021 aatttttaaa tttatacata gttatagtat ttagaagaat cgatttcctg ataataaatg
     7081 atgagatatg tatgattttg tggcaaactt ttaaaaaaaa cacctatatt ttgtgttata
     7141 atctctttaa aacgaatatg ctgaaaagag ggatttaaat gggaaaaata aattttgatt
     7201 atagtaaagc tacagagttt ttttgtcaaa acgaaataga tgtcatgcaa ccatatgtag
     7261 atgttgcgca cgacatgtta cataataaaa ctggtttagg aaatactttt ttaggatgga
     7321 tagaccttcc taaaaattat gataaagaag agtttgacag aataaaaaaa tctgctgata
     7381 agataaaatc agactcagat gttcttttgg taataggtat aggaggctct tatttaggtt
     7441 cgagagctgc tatagatatg gtaagtcatt cttttaggaa tggacttaaa aaagagcaaa
     7501 gaaaggctcc agaagtatat tttgtagggc ataatataag ctcaacatat attatggatt
     7561 tattagatat aattgaagga aaggatattt ctgtaaatgt aatatctaaa tcaggaacta
     7621 ctacagaacc agctctggct tttagaatat ttaaagatta cttagagaaa aagtatggta
     7681 aggaagaagc aagaaagaga atttatgcaa caacagatgc aagtaaaggt gcacttagac
     7741 agttagctac agaagaagga tatgagactt ttgtaatacc agatgatgta ggtggtcgtt
     7801 tttctgtatt gacagcagtt ggattacttc ctattgctgc tgctggactt gatattgatg
     7861 ctatgatgaa aggcgcaaat gatgctagag aagcatttca aaatcctgac ttaaaaagta
     7921 atgatagtta tagatatgct gttgctagaa caattttaca tagaaaagga aaagatgtag
     7981 agttattagt gaactatgaa cctcaactac attatgtaag tgagtggtgg aaacagcttt
     8041 atggagaaag tgaaggaaaa gaaaataaag gtttattccc tgcttctgta gatttctcaa
     8101 cagatttaca ttctatgggt caatacatac aagatggaaa aaggctttta tttgagacag
     8161 tacttaatgt tgaaaactgt aagagaaata taactataag ttcagaggaa gtagacttag
     8221 atggtttaaa ttatttagca ggtaagactg tagattttgt aaatcataaa gcttttgaag
     8281 gaactctatt ggctcataca gatggaaaag tacctaattt agtaataaat atacctcaac
     8341 tagatgagta taattttgga tatttagttt atttctttga gaaggcttgt ggaataagtg
     8401 gatatttatt gggagttaat ccatttgacc aaccaggagt agaagcttac aagaaaaata
     8461 tgtttgcttt actaggcaaa ccaggttatg aaaaagaaaa ggaagagcta gaaaaaagac
     8521 ttaaataatt ttaaatagtt aaggtttaat taagattaaa ataatataat gaaatcatca
     8581 aaaaggagtt tactcaaaat agatgatttc atttttttta tgaaaaattg ggaaaaatat
     8641 attttgataa tttatattat attaagatag ggagtggtat gagatgtcaa gaagaaagaa
     8701 agggataagt ttagtaatat tagttgctat aataagttca atacttagta gttttctaac
     8761 aataatttta gttaaggaca atttagtaag caaatctaca ggaagttcta cgccgatagt
     8821 tgtaaatgat gatggtaaga gtcaaaatat ttatcaagca gttgcagaaa aagctacgcc
     8881 atctgttgta ggtattacaa caactagtgt tgatactagt aatatgtttg caataccaac
     8941 tgaaacacaa ggtgtaggaa ctggaatcat agttgattca aatggatata tacttactaa
     9001 ttctcatgtt atatcagatg gtcaagctac aagtgtaaat gtattattca atgatggttc
     9061 aacaacttca ggtaaagtag tatggtttga ccaacaactt gatttagcta tagttaaggt
     9121 tgataaaact ggactaacac cagcagaatt tgctgatagt gacaaagtaa aagttggaga
     9181 tatatcaata gcaataggta atcctcttgg attagatttt caaaaaacag ttactcaagg
     9241 gataataagt ggattagata gaactataca aacagaaaaa actaatatga ctggtttatt
     9301 acaaactgat gcaagtataa atgcaggaaa tagtggagga ccattgttaa atcaaaaagg
     9361 tcaagtaatt ggtataaaca cagcaaaagc ttctcaagca gaaggattag gatttgcaat
     9421 accaatcaat acagctaaga gtatagttga agaagtaatc aaaaatggaa aatatgaaaa
     9481 agtaacttta ggcataaaag gtacagatgt atcaaattat gaagcagcaa caggtacaaa
     9541 attaagtact gataaaggtg tttatgttgc agaagtaata tctggttcat cagcagaaaa
     9601 agctggagta aaggtagggg atattataac taaagtagga gatacagata taacaggtat
     9661 gaatgattta aataaaaagt tgtatacttt ctcaaaaggt gctagtacaa aaataacagt
     9721 aaatagaggc ggaaaagcag taacaataaa tgtgaatttc taaatatgac caaaaagata
     9781 gagactacat ggtaaagtgt agtctctatt tttatttaat ttttatagta tatacatcta
     9841 aattaattaa aatgatgtat tgtttatagt ttattgctat attttcttat aaaaataaat
     9901 ttaataaata tttaaattta atttacaaaa acaatataaa tatcataata aaataaaatt
     9961 ttttaatatt actaaaaata tatagacaaa ataataacaa atatagtatt atgtaattgt
    10021 agaaatgctt ttcataggta tattctatga agatgaattg cataaaaatg aaaaaatgga
    10081 atggatatta tttaggggtg aaaaatcatg aatccattag ttataaactt acaaaaatgt
    10141 agcatacatg atggaccagg gataagaagt acagtatttt ttaaagggtg tccacttgaa
    10201 tgtgtttggt gtcataatcc agagagtcaa acatatacaa aacaagtttt gtataatgaa
    10261 gaaagatgtt caaaatgtga agcatgtata aatatatgcc ctcataaggc aatttacaaa
    10321 ggtgaaacaa aaatatgctt agaccaagat aagtgtgagt tttgtgaaac ttgcttagac
    10381 tattgtgtaa acaatgcaag agagattgtt ggtcaagagt attctgtaag agatttggtt
    10441 aaagagatag agaaggatag aattttctat gaagaatcag gtggtggagt aactctatca
    10501 ggtggagaag taatggctca agatatggac ttcatttgtg gagtaataaa catgtgcaaa
    10561 tcaaagggaa tccatgtagc aattgataca tgtggctatg caaagtcaga aaattatgaa
    10621 agagttgcta aatgtgcaga tttgttttta tatgatatta agcttattga tgaagataag
    10681 cacataaaat ttacaggaaa atcaaatgac ttaatactga aaaatgttaa gattttaagt
    10741 gaattagggg ctaacataaa tataagaata ccattaattg taggagtaaa tgtagatgat
    10801 gaaaatttag aggttaaaaa aatgatagag tttttaaagc ctttgaatat acaagcagta
    10861 agtcttcttc catatcataa tattggtaaa cacaagtatg ataaaattta taaaaagtat
    10921 gaaggagaag aactacaaag accatcagaa gaaaaattag aggaaataaa aagactgttt
    10981 gaagcaagta atttcaatac taagattgga gggtaataaa atggcaagag gaacttttga
    11041 gagaactaaa aaattaagag aagaaagtat taatgcagaa ccacacatat ctattgaaag
    11101 agcagtactt atgacagaag cttataaaaa atatgaggga agtgtagaga ttcctgtact
    11161 tcgtgcttta tcatttaagc attatattga gaatagaact cttagcataa atgatggaga
    11221 attaatagta ggtgaaaaag gtgattctcc taatggagca ccaacatatc cagaaatttg
    11281 ttgtcataca atggaagatt tagaagtaat gcacaataga gatataataa attttagcgt
    11341 atcagaagaa gctagaaaaa tacataaaga agaaataatt cctttttgga aaaagagaca
    11401 aacaagagat aagataataa atgcaatgac acctgagtgg ttagcagcat atgaagcagg
    11461 tatgtttaca gagtttatgg aacaaagagc cccaggacat acagtttgtg gagatacaat
    11521 atataaaaaa ggatttttag atttaaagaa agacatagaa gcaagattaa aagagctaga
    11581 ttttcttaat gatttagatg catataataa aaaagcagat ttagaagcta tggcaatagc
    11641 ttgtgatgca atggttatat taggtaaaag atatgctgag aaagcaagac aaatggcaga
    11701 agaagaaaca gatgaagcta agaaacaaga tttattacta attgctgaaa cttgtgatgt
    11761 tgtaccagct cacaaaccag aaacttatca tcaagctata caaatgtatt ggtttgttca
    11821 tataggggtt actactgaac ttaatatatg ggatgctttt actccaggaa gacttgacca
    11881 acatctaaat cctttctatg aaagagatgt agaaaatgga atactagata gagatagagc
    11941 acaagaatta cttgaatgtt tatgggttaa atttaacaat cagccagcac caccaaaagt
    12001 tggtataaca ttaaaagaaa gtagcacata tacagatttt gcaaatataa acactggtgg
    12061 aataaatcca gatggtcaag atggtgttaa tgaagttagt tatataatac ttgatgttat
    12121 ggatgaaatg aaattgatac aacctagttc aaatgttcaa ataagtaaaa agactcctca
    12181 aaaattctta aagagagctt gtgaaatatc aagaaaagga tggggtcaac cagcatttta
    12241 taatactgaa gcaatagttc aagagcttat ggaagctgga aaaactatag aagatgcaag
    12301 acttggtgga accagtggtt gtgttgaaac tggatgtttt ggtaaagaag catatgttct
    12361 aactggatat atgaacattc caaaaatact tgaattaaca ttaaataatg gatatgaccc
    12421 aattagtaaa aaacaaatag gtattgaaac tggagaccct agaaatttcc aatcttatga
    12481 ggaactattt gaagcattta aaaagcaatt acattatatg attgacataa aaatagaagg
    12541 aaatgctgtg atagaaaata tatgtgcaaa acacatgcct tgtccactta tgtctacaat
    12601 agttgatgat tgtatagaaa aaggcaagga ttaccaaaga ggtggagcaa gatacaacac
    12661 tagatatata caaggtgttg gtataggaac tataacagat agtttaacag caataaaata
    12721 taatgtattt gataagaaaa aattcgatat ggatacattg cttaaagcac ttgatgctaa
    12781 ttttgaagga tatgaagcta tacttaattt agtttcaaat aagactccta aatatggaaa
    12841 tgatgatgat tatgctgatg aaataatgca agagatattc aatgcatatt acaatgaggt
    12901 aactggtaga ccaactgttt gtggtggaga atacagagta gatatgttac caacaacttg
    12961 tcatatatac tttggagaaa ttatgggagc aagtccaaat ggtagacttt gtgcaaaacc
    13021 agtttcagag ggaatttcac ctgaaaaagg tggagatact aatggaccta cagcagttat
    13081 aaaatcttgt gctaagatgg accacataaa aactggtgga acattattaa atcaaagatt
    13141 tgcaccatca gtagttcaag gagaaaaagg gttagataat atggctaatc tagtaagagc
    13201 atactttaat atggatggtc atcatataca atttaatgta tttgataaaa atgtactgtt
    13261 agaggctcaa aagaatcctc aggattataa agacttaata gttagagttg caggatatag
    13321 tgaccatttc aataatttaa gtagaacact acaagatgag ataataggaa gaactgagca
    13381 aacattctag gtttgcttgt aaataaaact tcttactata taaaatcgta actgatataa
    13441 taaagtataa atggtttgat tccattttta aaattttaga ttaattatga gctaactttc
    13501 taaataggaa gttagctcgt taagtttaat gaaaaaatcg ttaaatttat atttagtaaa
    13561 tcatacacat attagtaagt aatacataca aaagaataat ttattatagt tataataagg
    13621 ttatatttta taaaaataag ataattttaa gcagttaaaa taaagtcaat ttagctactt
    13681 taaaataaaa ctaatagaaa aaaggagatt tgatatgaaa actttaggat ttattggttc
    13741 agggaatatg ggaagtgcaa tgataggtgg catagtacag tcaggattag taaaggcaga
    13801 agatataact gtatcagatt taagccaagt tgccttagat aaagtaaaag aagaatttgg
    13861 agtaaatact acaactgatt caaaggtagc tgttacaaat tctgatgtgg taatagtagc
    13921 acttaaacct aatatatgcg aaaaggcttt aactccactt aaagaattaa tagatgcaaa
    13981 taaaataata gtatctatag cagcaggtaa aactatagaa agtctggaaa gttttatagg
    14041 ttcagataaa aaaatagtaa gagtaatgcc taatactcca gcccttgttg gcgaaggaat
    14101 gtcagctctt tgtaaaaata gtaatgtaac agatgaagag ttaaatatga ttaaggctct
    14161 gtttgaatct tttggtgagg cagaaatagt atcagaatat ttaatggata cagttacagg
    14221 tattagtggt tcttctcctg catatgtatt tatgtttata gaagctatgg cagatgcagc
    14281 agtattagct ggaatgccaa gacaacaagc ttataaattt gcatctcaag cagtaatggg
    14341 gtcagctaag atggttcttg aaactggaaa acatccaggt gaattaaaag atatggtttg
    14401 ttctccagca ggaactacta tagaagctgt taaggtatta gaagaagaag gctttagagc
    14461 agcagttata aaatctattg ttgcttgtat agataagtct aaagatatga gtaaataaca
    14521 gatgtaatta agtttactat atggtttctt gaattgtttt tttagtgctt atttatatct
    14581 atacataaat gtatttggta taagtaaaat aagagtaatt tactataaaa aggaatttaa
    14641 tgattgagtg agaatttatt acttaaatat taataattaa atagatatat ttttaactat
    14701 atttgagata tatcattgtg aaaaaataaa atttataagt attattgtga ggttataagt
    14761 agatttattt aaaaataaat ttatttatag cctttttaaa tttgaaataa tattcataag
    14821 gtaataaaag ttacaaatat tttcttcgct aataagtttt aaaataccta taatgtt
//
LOCUS       DAEMGG010000100        14768 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEMGG010000100 DAEMGG010000000
VERSION     DAEMGG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.100"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            76..1341
                     /locus_tag="KPK24_002772"
     CDS             76..1341
                     /locus_tag="KPK24_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG2300425.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRKLYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            1356..2642
                     /locus_tag="KPK24_002773"
     CDS             1356..2642
                     /locus_tag="KPK24_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG2300426.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            3036..3782
                     /locus_tag="KPK24_002774"
     CDS             3036..3782
                     /locus_tag="KPK24_002774"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG2300427.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            3812..4747
                     /gene="rsmH"
                     /locus_tag="KPK24_002775"
     CDS             3812..4747
                     /gene="rsmH"
                     /locus_tag="KPK24_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG2300428.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            5228..5575
                     /locus_tag="KPK24_002776"
     CDS             5228..5575
                     /locus_tag="KPK24_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300429.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            5587..7566
                     /locus_tag="KPK24_002777"
     CDS             5587..7566
                     /locus_tag="KPK24_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG2300430.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILGE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIEAFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            7733..9106
                     /locus_tag="KPK24_002778"
     CDS             7733..9106
                     /locus_tag="KPK24_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG2300431.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            9147..10115
                     /locus_tag="KPK24_002779"
     CDS             9147..10115
                     /locus_tag="KPK24_002779"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG2300432.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            10134..11489
                     /locus_tag="KPK24_002780"
     CDS             10134..11489
                     /locus_tag="KPK24_002780"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG2300433.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASVIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            11541..12671
                     /gene="spoVE"
                     /locus_tag="KPK24_002781"
     CDS             11541..12671
                     /gene="spoVE"
                     /locus_tag="KPK24_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG2300434.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            12689..13915
                     /gene="murG"
                     /locus_tag="KPK24_002782"
     CDS             12689..13915
                     /gene="murG"
                     /locus_tag="KPK24_002782"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG2300435.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            14185..>14768
                     /locus_tag="KPK24_002783"
     CDS             14185..>14768
                     /locus_tag="KPK24_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBG2300436.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEILT"
BASE COUNT         5957 a         1463 c         2792 g         4556 t
ORIGIN      
        1 tgtgagtata tgtaattaaa aaataaataa atttcaattt taaaattgat gtttaaataa
       61 aaataggagg tacatatggt taatactaaa aaaataataa atttaggtaa taatattaat
      121 cttactttga taaaaacaga aaagtttaaa tcgaatctta taagtttata tgttcagaga
      181 ctattggatg aaaaagaaac tacaaaaaat gctctaatac catcaataat agcaagtgga
      241 agcgctaaat atcctagtgc tagagcaata tccaataaat tagatgattt atatggttca
      301 tctatgggag ctgatgctgt aaaacgtgga gaacgtcaag tattgagttt taaagtaatt
      361 aacataagtg aaaagtattt agatgagagt atatttgaag aagtagtaga attttttaat
      421 gaagtaataa ataatacgct tgttgtagat ggtgggttta aagaagaata tttaaatatt
      481 gaaaaagaga atttaagaga aaagatacaa tcaataataa atgataaaaa ggaatatgcg
      541 caagataaat gtattgaggc tatgtgtaaa ggagagagat atagtgtaag tgaatttggt
      601 tatgaagatg aaatagatag tataacttca agaaagttat atgaacatta taaaaacata
      661 cttaaaacat cacctataga tatagttgta gaaggtaatt ttgatgaaga taaagttgta
      721 gatataataa gtaaaaatct aaaatttgaa agagaagaaa ttataaatat accaagagca
      781 gattttataa aaaatgtgga tgaggtaaaa gttatagatg aacaaatgga aataactcaa
      841 ggtaagttag ttatgggtta tagagcaaat gtggattatg cagacataga taaatattat
      901 gctttagtag ttgggagcaa tgtcttaggt ggtggtccac actctaaatt atttgttaat
      961 gtaagagaaa aagaaagttt atgttactat atattctctt caatagagaa gtataaaagt
     1021 atcatgttta tatcatctgg tatagaaact aaagattatg ataaagctgt agaattgata
     1081 aaaaaacaag tagaaagtgt aaaagcaggt gatatatctg atgaagaatt agaaaacagt
     1141 aaacttgctt tagttaattc tatgaaatct ataactgata atataggtgg tatgtctgat
     1201 tttgttttct ctcaatctat ggcaaagaca aattctgaag tacaagatat aatatctagt
     1261 atagaaaagg ttacaaaaaa agatatagtt gaagctataa aaaatataga gcttgatact
     1321 gtatacttct tgagaaatta atggaggaga tgtctatgga aaaaatagtt aatgatatat
     1381 tgaaagaaga agtttattat gagaaattgc aaaatggatt agatgtatat tttatgccta
     1441 aaaggggttt tatgaaaaag tatgctattt tggctactaa ttatggttca aatgacttag
     1501 aatttgtacc aattggagaa gataagaaaa ttagagtgaa tgaaggtatt gcacattttc
     1561 tagagcataa gatgtttgag caacctgatg gaggagatgc atttgataaa ttttcaaaat
     1621 taggagtaaa tgcaaatgca tttactaatt ttacaatgac agcctactta ttttctgcta
     1681 ctgaaaattt ttatgagagt ttagaacatc taattgacta tgttcaaaca ccttatttta
     1741 cagatgaaaa tgttgaaaag gaaaaaggta taatagcaca agagataaaa atgtacaatg
     1801 atgaccctga ttggaatgta tactttaatt gcttgaaggc aatgtatgta aattatcctg
     1861 ctagaattga tatagcaggt actgttgata gtatatataa gataactaag gaagagctat
     1921 ataaatgtta taatactttc tataatccag gaaatatggc tttatttgtt gttggtgact
     1981 tagatgtaga aaaagttata gatgtaacta aaaagtcaaa caattacaaa gtagacaaac
     2041 tctctaagag tatagaaaga ttttatccag aagaaccaga aggtgtaaaa gaaaaagagg
     2101 ttatagaaaa attccctata tctatgccta tgttcaacat aggttttaaa gatagtaatg
     2161 ttggtttaaa agggaaagaa cttttacgta aagagattgt aactgatata cttgtaggga
     2221 tgttatttaa aaaaggaagt aagctttatg aagatttgta tatgcaaggt ttaataaatg
     2281 aaaattttgg agctggattt tcgtctcaag tggattatgc ttttagcata atagctggag
     2341 attctaaaga gcctaaaaaa gtaaaagaaa taattcttga ttatatagaa aaatcaaaaa
     2401 aagaaggatt atcaaaagaa gagtttgaga gaactaaaaa gaaaaaaata gggtcattta
     2461 ttaagtgctt tgattctata aactttatag gtaatagttt tatatcttat gtttttaagg
     2521 atataaatct attggattat ttagatatta ttaaagatat tacttttgag gaagttgaag
     2581 aaagattaaa agagcatttt aaagaagagt attgtgttat ttctattgta gagcctaaat
     2641 agaaacttaa tcataataag aataactaat taagtattag tttatgtttt taaatgttta
     2701 ttttggtaaa acaaatttat aaaatatatt tatataaatt gaggtaaaaa tttaagagct
     2761 atagataaga aatagaagag gttgatgtat atatgcatca atctttttga ttaaaaaata
     2821 tctatgtaca cttgtaactt tagtcatgag agattcagtt taaaaaattt tatagtaatt
     2881 agatataata tagtgtaggg agttgtagaa taaattttaa tattttttgt agaattaaca
     2941 ttaatgtgtt atcataattt agtataatta caattaattg taattatagc atattgcttt
     3001 ggaaatattt tcaattaata caaagaggtg acgttatgga tagagtagca tttacactgt
     3061 ttggcataga catcatgtgg tatggaatac ttatggcatg tggaatgatt ttaggaactt
     3121 taatagcaat aaaagaagca aaaagagtgg gaattaaaga agatgatgta ttaaatatag
     3181 ctataatagc aattccagtt ggactaatct gtgcaagaat ctattatgtt gtatttaatt
     3241 ggagttacta tgctcaaaat atgtctcaaa tttttaattt tagaggtggt ggccttgcta
     3301 tacatggagg gttaattggt ggaatattag ctggttatat atacactaag attaaaaata
     3361 ttaacttctt aaaaatggct gatacagtta tacttggaat gcctttagca caagctattg
     3421 gaagatgggg aaattttatt aatggagaag cacatggagg tgctaccaat ttaccttggg
     3481 gaataatggt tgatggagta aaggtacatc caactttttt atatgagtct atatgggatt
     3541 ttggaatatt tatagtttta ttactattta gaaaaaataa aaaatatgaa ggtcaagtta
     3601 tagttactta tataacttta tattcaatag gaagattttt tatagaggga ttaagaacag
     3661 atagtttaat gttaggacca cttagaatgg cacaagttat aagtctaatt ggagttattg
     3721 gtggcataat tgcacatgtt tatttatcaa agaaaaataa aaataatatt agtgaagaat
     3781 agtttaaaaa gtgaatttaa gggaggtaac tatggaattt catcatgtat cagttctttt
     3841 aaatgagtgt atagagaatt taaatataaa gccagatggc gtgtatgttg actgtactat
     3901 gggaggagca ggacattcta aagaaatagt aaaaaaactg tcagataagg gattatttat
     3961 agggtttgac caagataaaa atgctataag cacagcaaaa gagagattga gtgaatatga
     4021 aagtagagtg aagtttgttc acagcaattt tgaaaatata aaagaggaat tagaaaaaat
     4081 aggagtttac aaaattgatg gtgtattagc tgatttagga gtttcatctc atcaacttga
     4141 tgaagctgat agaggatttt cttatatgca agatgcacca cttgatatga gaatggatgt
     4201 taggtgtgag ttttctgcat atgatgttgt aaatacttat acagaagatg aattaactaa
     4261 gataataaaa gattatggag aagataactg ggctaagcgt atagctaaat ttatagttga
     4321 agaaagagca aacaaaccaa tagaaactac aggagaatta gtagatgtaa ttaaaaaagc
     4381 tatccctaag aaagctagaa tagatggacc tcacccagct aaaagaactt ttcaggcaat
     4441 acgaatagaa gtaaataatg aacttggagt aattacaaag atgataaatg atgcttcttc
     4501 tataatgaat gaaggtggaa gaatatgtat aataacattc cattcattag aagatagaat
     4561 agtgaaaaat gcatttaagc atttagcatc agattgtata tgtccacagc atttacctat
     4621 atgccaatgt gataaagaat ctgaggtgaa aataataact agaaaaccta tattacctag
     4681 tgaggaagaa atagaagtaa atccacgttc aagaagtgct aagctaagag tagcagaaaa
     4741 aatataagta gttataaggc taaagaataa tcttatatat aagattatta taattaatta
     4801 aaaagtatta tatattttgt tgcactggta aagcaattaa aaaacaatta acactataag
     4861 aaatgttctt tgttattgag atataaaatg aaaatttaga tataaaaata tagatataga
     4921 tggtattaag taatataatg gagaacttag gaatcagaga gtagatagtt agttacatat
     4981 tatatttatg gaagaaatta tctaaaaata ctagaagtga taaaaataaa tagaaattaa
     5041 tatctaacct atagattcat ataaaagtat aagtgaattt ataggttttt gttgtgtata
     5101 gaaaaaaata caattagatt gtgtaatagt gtattaattt agcgatgaaa ttttagagga
     5161 agaaatatga ttaataacaa atatagtata gaggaaaatt taaactatat actatcgggg
     5221 tgagaatttg aaaaaaaatt ataaaataca taatctaaat gaatataaaa ataaaaaaaa
     5281 gaataattat aaaaagagaa ataagaaaaa aataggagct aaatgcattt tactgattgc
     5341 aactttttca gtaataatat tgaatctatg tggttattca aaaatctcgg aaatgaagta
     5401 cgatatctat tatttacaaa aagatttaag aaaaaaagag atagtattag aggaactaaa
     5461 ggcaaaagaa actgaaaaaa catcaataaa agaaattgag ctaaaggcta aggaattgct
     5521 caatatggac tatccaaaag atggacaaat aaagtatata gatgtggata actaggggga
     5581 atagatttga gaaaagtaaa gaggataagt aagaaaaggc tggtactcgt tcttattcta
     5641 gcatgtgcat tgtttttttg ccttgttatt aggactgggt atctacaact tatgaaggga
     5701 aattggttga gtacaaaagc actagaacaa caaacaagag atataccaat agaacccaaa
     5761 agaggaacta tatatgatag aaatatgaaa gagttagcag taagtgtaac taagtatact
     5821 gtttggtgta aacctgtgga agtagaagat aaaaaagaag cagccgaaaa ggtagctgag
     5881 attttgggtg aagattataa agatatttac gctttaataa gcaaaaaaaa tatggctctt
     5941 gtaaaagtaa aaagatggat agatgatgat aaggcaagtc aaataagaga tgctaaatta
     6001 agtggaatat gggtagcaga agataatcaa agatattacc catatgggaa ttttgcacca
     6061 tatgtgttag gacatacttc ttcagatgca acaggtatat ctggtgtaga aatgcaatat
     6121 gataaaaagt taaaaggaaa gcctggaaaa cttatagtaa gtactgatgc ttcaggaaga
     6181 gaaataccac aaggaatgga aaaatattat gagcctgtac aaggtaatgg tcttgtgttg
     6241 tctatagatg aagtaattca acactatact gaaaaagcag tacagaaagc ttatgagtta
     6301 aataatgcaa aaaaagttac tgcaatagct atgaacccta aaacaggtga tatacttgct
     6361 ttagcatcaa aaccagatta tgaccctaat gattcaagaa cgccaatata tccatattat
     6421 caagaagaac ttgaaaaata taatgataaa gataaaataa aaggctatta tcaaatgtgg
     6481 agaaatccag cagtaagtga tacatatgaa ccaggttcta catttaagct tattacatct
     6541 tccagtgctc ttgaagaagg agtaattaaa gatggagaaa aattcacatg tacaggtagt
     6601 gtaactgttg gaggaagaaa gattaaatgt tggagacatt acagacctca tggaacacaa
     6661 gaatttaaac aagcagtaca aaactcttgc aacccagtat ttgttgagtt aggaagcaga
     6721 ttaggtgttg gaaaaatgta tgattatata gaagcatttg gtcttatgga taagacaggt
     6781 atagatttac caggagaagc taagggaata ctgtataacg aaaaaaatgt aggaccagtt
     6841 gaattggcta caatatcatt tggtcaatct atatcagtaa ctccaataca acttataaca
     6901 gctatatcat caatagctaa tggtggagat ttaatgcaac caagagtggt aaaatcttat
     6961 actgacaata aaggaaatat aactgaaaca gtaaaaccaa aaaaagtaag aagtgttata
     7021 tctaaagaaa catctaaaaa aatgttagaa attgcagaat cagttgtaac tgaaggtgga
     7081 ggaaagatag catatatacc aggttataga cttggtggta aaacaggtac agctcaaaaa
     7141 gttatagatg gtaagtatgc tccaggaaag tatatatgtt catttgttgg aatagcacct
     7201 tgtgatgacc cgcaaatagt agttcttgct attgttgacg aaccaacagg agtaagtgca
     7261 tttggtagta ctacagcagg accaatagtg aaagaaataa tgaatgattc tttaaaatat
     7321 ttgggagtta aaccagtata taaagaagaa gaaaaagctg agtatgaaaa gaagcaagtt
     7381 aaagtgccag atgtaagaaa tctaaaaata ggagatgctg taaaggcatt ggaggatgcg
     7441 aagttaaaac cagacttaga tgcagatata gaacttccag aagatacaaa ggtaaaggat
     7501 atattcccaa aacctggagt aaaagttaat gaagactcaa gtataaccct atattttgaa
     7561 aactaaaaag taacagataa ttaacatata cccataaaca tcttcaaaaa aacagaaata
     7621 tagtatataa ttataaagtt aagagttatc gtaggcaatg tatatataac ggtatacatt
     7681 gccttttgtt gctaagagaa tatagctaag gttttaaagg aggagtttaa taatggagtg
     7741 cttaacaata aaagagttag tacttgcaac taatgggaag ttaatatatg gagattataa
     7801 tgactgtgta agtgatatag ttatagatag cagggaggca aatgcccaaa atgcatttgt
     7861 agccatagtt ggagaaaatt tagatgggca tacatttatg aagtctgcat atgatagtgg
     7921 atgtaaaact tttataaaaa atgaaagtaa tggaataaaa ttagaaagtt cggatataaa
     7981 tttaatagag gtaaaagata catctttggc tctaggagat atttcaaaat actataaaga
     8041 aaaatttgat attccattta taggtgttac aggtagtgtg ggaaaaacta ctactagaga
     8101 tatgatttac tctgccatat ctgcaaagtt aaatatacta aaaaatgaaa aaaacttaaa
     8161 taatcatttt ggagttccat tgactttatt taatctaaat aaagaacatg agtgtgctgt
     8221 aatagagatg ggtatgtctg gattcaatga aataaaatat ttagtggata tagtaaatcc
     8281 taaaattgca gtcatatcga atataggatt atcacatgta gaaaaactag gttcacaaga
     8341 gggtattttg aaagcaaaga tggagattac ttcaaacttt gatgaaacaa atactttaat
     8401 tgtaaatgga gatgataagt ttttatctac attaaaagaa aaagaacatg tatataagtt
     8461 aaaaacattt ggctttaata aaaacaatga tgtttattgt gaaagttata ctatggaaga
     8521 ggatagttta acatttatat gtgtaataaa tgggaaaaaa gaggaaatat ttataccaac
     8581 aataggagaa cataatatat ataatgcaat gtctgctata ttagttggtt tgtgtctaaa
     8641 tatttcttta gattatataa aaaaaggttt aaaaaatttt aaagggacaa aaatgagact
     8701 agacataata aaaaatgaaa aattaacaat aattaatgat tcatataatg ctagtccaga
     8761 ttctatggat gctgctttaa agatacttgg aagatataaa ggtagaaggg tagcgatact
     8821 tggagatata ctagaaatgg gtgaaatttc agagtatggt catagattag ttggaaaatc
     8881 atctatgaat aatactgata ttataataac tataggtgaa aattcagtgt ttattggaga
     8941 agaagcaaaa caattaggat tcaattcagc caatatatat cactttgaga atagagaaga
     9001 tgtgtttaac aagttaaatg aattagtaaa aacaggagac actattttag taaaaggctc
     9061 aagaggcatg agattagaaa aaatagtcga atatctaaat aaataagctt aaactaatga
     9121 gttaattgac aagggagagg agttttatga tgttaggaat aacagagctt acgtacaccg
     9181 cattgattgc gtttttaata gtaattataa taggaccaat atttatacca atgcttagaa
     9241 aatttaaatt tggtcagaca gttagagatg atggacccca aacacatcta gctaaaaacg
     9301 gaacacctac tatgggtgga attataatga ttgttgcaat tctaataact ggacttacaa
     9361 gagtaaaagt tagtcatgat atggcagtag gtcttatatg tattgcaggt tttggtttta
     9421 ttggcttttt agatgatttt ataaaaataa agctaaaaag atcacttgga ctaaaagcat
     9481 atcaaaaaat aatccttcaa gttgcattat ctttttatgt tgctttttat cagtatacat
     9541 catcatcaag tgcttcacaa cttatgatac catttacaga ttttgtgata aatgttggaa
     9601 tattatatat accaataatg atgtttataa tcgtagcaat agtaaatgct gtaaacttga
     9661 ctgatggatt agatggatta gcatcaggag taacattaat agtttctgta ttctttatgc
     9721 tatttgcaag ttctattgct ggaaatacag aagtcgcagt tttagcagca gcaactgttg
     9781 gagcatgttt aggtttctta ggatttaatt catatccggc tagagtgttt atgggcgaca
     9841 caggttcaat ggcattagga ggtgcagttg tagcattttc agttctaact aactcagtgc
     9901 ttataatacc tataattgga ggaatttatt ttgcggaggc tctatcagtt ttgattcaag
     9961 ttggatattt taaggcaaca agaaagagat tctttaagat ggctccaata caccaccact
    10021 ttgaacagtg tggttggcca gaaacaaggg ttgtatttat tttttggatt ataacagttg
    10081 ttttagcttg gataagtata atagcagtat tttagggcta gggggaatga aaaatggact
    10141 taaaaggaaa aaaagtttta ttagtaggac ttgcaaaaac tggtatatct acaattaaac
    10201 atttagataa attgggtgca agtgtaatcg taaatgatat aaaagatgaa aataaattga
    10261 gaaatatact tgatgaattg aaatctataa atgacataaa atacatatta ggacatcacc
    10321 cagaagatgt agatgatata gatatggttg tagtgtctcc aggtgtacca ctagacctac
    10381 cattcatatt gaaattaaaa aattcaggca agtatataat tggtgaggta gaattagctt
    10441 ttaaactatc gaataatcca atttttatag ggattacagg tacaaatgga aagacaacta
    10501 caactagttt ggtaggagaa attttttcaa gagcaaaacg agatacatat gtagttggta
    10561 atataggaaa tcctgtaata gatactatag aaacttcaag tgaagagtct gtgttagtaa
    10621 ctgaactaag cagttttcag cttgagagta tagatgaatt tagacctaaa gtaagcgcaa
    10681 tattaaatat tacagaggac catttaaata gacaccatac tatggaaaaa tacatagaag
    10741 caaaggcaaa tatatttatg aaccaaactg ttgaggattt ctgtgtatta aactatgatg
    10801 atgagatagt aaaatcttta gctgataaat gcaatgccaa agtaatttac ttttcaagaa
    10861 ctaaaaaagt aaatggagga gtatatttag aaaataatga cataataata gatatagatg
    10921 ataaaatcaa atttctaaat aaagatgatg taagtttacc tggaggtcat aacttagaaa
    10981 attgtatggc agctatagca atagcatatg tctgcaaaat tgatttagaa gttataaagg
    11041 atgtacttat gacatttaaa ggagtggaac acagacaaga gtttgtaaga aatttagaca
    11101 atgtaatata tgtaaatgat tcaaagggaa ctaatccaga ttccactata aaagctatac
    11161 agtcttatga tagacctata atacttatag caggggggat ggataaggga agtaattttg
    11221 atgaacttct tgaaactgca aagtcatatg ttaaatcatt ggtcttatta ggagaaactg
    11281 catcaaatat agaaaattgt gctaaaaata aaggatttaa tgacatacat atagtaaaag
    11341 atatggaaga agcagttaag acttcttatg aaatttcaaa aagtggagat atagtgcttc
    11401 tttcaccagc ttgtgcaagt tgggatatgt atgaaagttt tgaagtaaga ggaaaagact
    11461 tcaaagataa tgtaaacaat ttaaaataat ctcttacctc taaacttact taaatggaag
    11521 aatggaggta gattagttgg atgcctaaag aaaatttgaa aaaacaaatt gacataagga
    11581 tgaagactga atttgatgga gtagtgtttt acactactat gcttttagta tttgtaggaa
    11641 tagttatggt gtttagtgca agttttatac aatcttcatt taaacacaat gatgcgtact
    11701 attttctaaa aaagaatgta atatatgctg tacttggatt tatagttatg attattacat
    11761 caagaataga ttatagtttt tggaagaaaa atgctacagc aattggagct atagcagtag
    11821 tattattatt gttagttttg acccctttag ggatagaagc aaacggagca aaaagatggt
    11881 tgggaatagg agctttaaca tttcaacctg cagaaatcgc aaagtttgct acaataatat
    11941 taactgcaaa attaattgaa aaaaattatg ataaaataaa atctttgaca aaaggtgttg
    12001 taccacttct cgtagttcca gggatatttt ttgcgttgat aatacttcag ccaaatttgt
    12061 caacagcagg tactgtaatt ctagttactt ttgtaatgat atttgttgct ggaatggata
    12121 tgaaaattgt atttgctatg atagggagtg gggctgcttt atttgctgca ttagtaattg
    12181 ctgaacccta tagacttagt cgtgttacat catttttaga cccttttcaa gaccctcttg
    12241 gtaaggggta tcaggtaata caaggtctat atgcactagg ttcaggtgga ttgtttggtc
    12301 taggcttagg aaagagtaaa caaaagtatt tttatatacc agagccacaa aatgacttta
    12361 tatttgctat aataggagaa gagttaggat tgattggttg tataatagtt attatgctat
    12421 ttgtagtttt agtctacaga tgtgtaagaa tagctttgaa gacttctaat gtatttgctt
    12481 gtatggttgt tataggtata ggtgcacaaa ttggaataca agcagcttta aatatagcag
    12541 ttgcgacatc atcaatgcca gctacaggtg tagcactacc atttataagt tatggaggaa
    12601 cttcactcac catctttatg ggagctgtag gaatagtgtt aaacatctca aagcatgtca
    12661 aaattaatta acaggagatg atgatttgat gaaagtttta ttatcaggcg gtggaactgg
    12721 agggcacgtg tatccagcta tagctattgc aaacaaaata agagatgagc atccagacgc
    12781 tgagataatt tttgttggga cggaaaaggg aatagagtca gaaatagttc ctaagtatgg
    12841 atttgaatta aagactgtaa cagttcaagg ctttaagcga aaaatagatt tcgataatgt
    12901 aaaaagagtt tttaagcttt ttaaaggttt ggagcaatca agaaagattg ttaagaaatt
    12961 taaaccagat atagttatag gaactggtgg atatgttagt ggacctgttt tatttaatgc
    13021 atctatgggg aaaataccag ccataataca tgagcaaaat tcttttcctg gtgtaactaa
    13081 taagatttta tctaaaacag ttacaaaggt acttacaagt tttgaagatt cgcataaaag
    13141 atttccagag gcagcagaag ataaattagt ttttacagga aatccagtta gaaaagaaat
    13201 tttattatca agaaaaaata ttgcaagaaa gaatctatca ataagtgacg aaaaaagaat
    13261 ggtactttgc tatggtggta gtggaggttc aagaaaaata aatgatgcaa tgagacttgt
    13321 aataaagaat atggtaaatg aagatatagc ttttatattt gctacaggca aaagctatta
    13381 tgatgaattt atgggaagta ttagcgatat aaatcttaaa ccataccaaa aagttgtgcc
    13441 ttacttagaa gatatggcaa atgcacttgc agcaagtgat ttggttatag gtagcgcagg
    13501 agcaatttca ttagctgaga taacggctct tggaaagcca tctataataa tacctaaagc
    13561 ttatactgct gaaaatcatc aagagtataa tgccaagagt atagaaaaac aaggggcagg
    13621 aattgctatt ttagaaaaaa atctaactcc agagagccta aatacagcag tatttaaact
    13681 tttaggagat agagaacttt tggttgatat ggcaaatgca agtaaaacta taggtaagcc
    13741 tgaagccata gaccttatat atgatgagat aatgaaagta tacaattcaa cacaaaagtc
    13801 aacttctaaa aaaacaaaaa aagaaaaagt tattaaggaa gtaaaagaaa taaaaaaaga
    13861 aacgactcca agtatagaag gtcaagctaa ggtaatagga ataaagaaaa gataagtaaa
    13921 tatttacccc taatttataa tatattagat tttctaaatt attataaatt aggggtttat
    13981 ttgtatgtat acagatttaa atatataaat agtatgtagt atgctatgtt tattttgatg
    14041 aaatattgat ttatttataa aatttaatac cttatttgga tgttatgtaa taaaatgcaa
    14101 aaatttgact ttaaatatta taattttttt gtaggaaaaa caagctctaa aggtgtattt
    14161 attaaccaaa ggggttgatt atatttgaaa aaaagaagaa agttaaatac gaataatgtc
    14221 atgatagttt tagtttttat acttatgtta tctttttgta tatgtataat tgtaaaaagt
    14281 gatttatttg atgtaaagaa aatagatgta attggaaata agagggtgac caaatcaaat
    14341 ataatgaaag aactaaatgt aaatctaaat gaaaatattt ttgcatacaa ttttaaagat
    14401 atgaaaaata aacttataaa aaatccatat atagaaaatg tggaaataaa aagaaaactt
    14461 cccaataaga taattataag tttaaaagaa aaagaaatat ttgcagtatt aaaagatgaa
    14521 gataattatt gttatataga taaaaaagga aatttattgg aagaactaag aggttctaat
    14581 gaaagtaaaa aagacttgat agtagatgta gattatagca ttgatgataa taaatctata
    14641 aaatttaaaa attataaaac taaggagaat gtatttaaaa ctttaaatta tttaaaagaa
    14701 gaaggaattt atagaaaaat aaattatgta aacttaaaaa aagaaagcaa tatagaaata
    14761 cttacaag
//
LOCUS       DAEMGG010000101        14528 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEMGG010000101 DAEMGG010000000
VERSION     DAEMGG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.101"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..606
                     /locus_tag="KPK24_002784"
     CDS             <1..606
                     /locus_tag="KPK24_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2300437.1"
                     /translation="ISGYLTKKLQDLNVDTIRTDILTSPTVTDVIVWNIEQSGTDTFS
                     ATYEVDQQIKEGEQTTTVKATYTVKVHVDADRDMVIIQNPTLAPAIEKSDYEPKTPEA
                     DASVDADTVNDATAFLETFFKLYPTATAKELAYYVFGNVLEPIGRDYLYSELVNPVLT
                     KDGDNMKVKVAVKFLDNQTKATQVLQYELILHKDSNWKIIM"
     gene            819..1676
                     /locus_tag="KPK24_002785"
     CDS             819..1676
                     /locus_tag="KPK24_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2300438.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVTSLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            1754..2335
                     /locus_tag="KPK24_002786"
     CDS             1754..2335
                     /locus_tag="KPK24_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2300439.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            2439..3284
                     /locus_tag="KPK24_002787"
     CDS             2439..3284
                     /locus_tag="KPK24_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300440.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            3281..3964
                     /locus_tag="KPK24_002788"
     CDS             3281..3964
                     /locus_tag="KPK24_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300441.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIICFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVEHM
                     WKSLGGVKL"
     gene            3961..4659
                     /locus_tag="KPK24_002789"
     CDS             3961..4659
                     /locus_tag="KPK24_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300442.1"
                     /translation="MKKILSSTIQIFKQIKSDPMMFAACFSPFIMGTLIKFGIPFLER
                     ITKFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            4671..5582
                     /locus_tag="KPK24_002790"
     CDS             4671..5582
                     /locus_tag="KPK24_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2300443.1"
                     /translation="MEKGRLLQNKNMNKAIQVSKITLIINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVVSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVPSGIALTAAVLSIVIKEWMYWFTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            5634..5774
                     /locus_tag="KPK24_002791"
     CDS             5634..5774
                     /locus_tag="KPK24_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300444.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNRSKDSEDDK
                     MY"
     gene            6065..6691
                     /locus_tag="KPK24_002792"
     CDS             6065..6691
                     /locus_tag="KPK24_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2300445.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            6813..7529
                     /locus_tag="KPK24_002793"
     CDS             6813..7529
                     /locus_tag="KPK24_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2300446.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            7679..8275
                     /locus_tag="KPK24_002794"
     CDS             7679..8275
                     /locus_tag="KPK24_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBG2300447.1"
                     /translation="MKILWIIIIMADNLLPLLLARFYPNYKHKEMALSVLGSRQSPVK
                     WIYNVWCIISGVVFCIAPYALYQENDSGWAIAIWMLLAIYGIGCEIISGVCPLNENRQ
                     DEDIITKIHGGTSAIGFMALLVVPLLFAILQFQTTKVVLGAISLVCFTIAFIFFCFFI
                     MGEKEKFANTVLRYGGLWQRLVLISCYIPLFVWSVSNQ"
     gene            8362..8553
                     /locus_tag="KPK24_002795"
     CDS             8362..8553
                     /locus_tag="KPK24_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBG2300448.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            8577..8807
                     /locus_tag="KPK24_002796"
     CDS             8577..8807
                     /locus_tag="KPK24_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300449.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            9074..9493
                     /locus_tag="KPK24_002797"
     CDS             9074..9493
                     /locus_tag="KPK24_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2300450.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            9496..9750
                     /locus_tag="KPK24_002798"
     CDS             9496..9750
                     /locus_tag="KPK24_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300451.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            10125..10328
                     /locus_tag="KPK24_002799"
     CDS             10125..10328
                     /locus_tag="KPK24_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2300452.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRKLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            10406..11596
                     /locus_tag="KPK24_002800"
     CDS             10406..11596
                     /locus_tag="KPK24_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2300453.1"
                     /translation="MSKMKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPTGKRDDKSLREKEKQIKKDLDDGIDTIGGKMTVCQLYAKKNNQKKNIKRN
                     TEIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFHFKLSDVLEDDTEPKVILTPEQEEKLLSFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHE
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            complement(11853..12653)
                     /locus_tag="KPK24_002801"
     CDS             complement(11853..12653)
                     /locus_tag="KPK24_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300454.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(12848..14446)
                     /locus_tag="KPK24_002802"
     CDS             complement(12848..14446)
                     /locus_tag="KPK24_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2300455.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
BASE COUNT         4896 a         2354 c         2688 g         4590 t
ORIGIN      
        1 atcagcggtt atctaacaaa gaaattgcag gacttgaatg ttgatactat tagaaccgat
       61 atactgacta gccccacagt tacagatgta attgtgtgga atatcgaaca gtcgggaacg
      121 gacacttttt ctgctaccta cgaagtagat cagcagataa aagagggaga acagacaaca
      181 acagtcaagg caacctatac ggtaaaagtc catgtagacg ctgacaggga tatggtaatt
      241 attcagaacc ctacccttgc accagcaatc gaaaaatcgg actatgagcc aaagacacca
      301 gaagcagacg caagtgtaga cgctgatacc gtgaatgacg ctaccgcatt tctggaaaca
      361 ttctttaagc tgtacccgac agccacagca aaagagcttg cctattatgt atttggaaat
      421 gtgcttgaac ctatcggcag ggactacctt tattcagaat tggtaaaccc agtacttaca
      481 aaggacgggg ataatatgaa agtcaaggta gctgtaaaat tccttgataa tcagacaaag
      541 gcgacgcagg tattgcagta tgagcttatt ctacataagg atagtaactg gaaaattatt
      601 atgtaagaca ttttgttggc aggatctatt ttcggatata gtctgccttt tctgtagcaa
      661 atgtactgca ctgacaagct atttcattgt attttaagca attaaacctt tcttgacaat
      721 tttatcttaa atcatatatt tatggatatt gactttctgc ttgcaataag ctataatatg
      781 actataaaat caaatcagtc atataggagg tatgtggtat gtataaagaa gttggtattt
      841 cttcaaataa tgttccgata acactttcta tctggtatac agaaagaagt agtcctacca
      901 ttgttttttt gccgggtact atgtcacacc ctctcatgta tgagaatttt ctatgtggtt
      961 tgagtgaaag aggatttaat ataataggca ttcactatct ttcacacgga aaaagcccta
     1021 aaatcaaaaa aaattataca atggaagatt tgctgcataa tgtatatgac gcaacaactt
     1081 acggaataga aaatttcggt gaaaacatag ctgtcatggg ttccagtcaa ggcgggattt
     1141 tagctgcaat ggcggcaggt agggacacaa ggttaaaagc cgtttttcca cataatatca
     1201 tgctgaccac attaaaagaa actatgagct taacaaaata tcccacatgg tcgcaccgtt
     1261 ttatggggct gatacagtgg ggctttcgtg taggtggaaa attgctgcct aactacaagg
     1321 ttccaggaga cgcttattta gactatgaca gagtatttta tagtgaacag gcaaaacaag
     1381 attgcttaaa tgaccctatg cttttaccct attatccttt aagatttgtc accagcttat
     1441 ttaatgctga cttatcgtgc ctggagaatg gggctataca atgtcctgtt attttaatta
     1501 ctgccaaagg cgacccgctt tttagctttg attataccaa caaagtattt gatttaattc
     1561 atgtaccgca aaaggaatta cttgctttag agcttaatcg tcacatgatt tttaatgagg
     1621 acacggacac tacaataaac gcattggaag ctaccttgca taaatatctt ggatagcata
     1681 ttgattatat aatcagtaaa tgttatactt ttcatgacga tataaatatt ttagtcataa
     1741 ggaggttatg agtttgccac gatttacgga acaagaaaaa gaaatcataa acagtaaact
     1801 tttgatagag ggagaaaagc tgtttgcatt acacggctta aaaaaagtaa cagttgatga
     1861 tttagtagct gctgtaaaca tatcaaaagg ttctttctat gctttttatc ccagtaaaga
     1921 acatctatat gttgaaatca attttcgctt acaaaaagag ttatttacaa gtatagaaac
     1981 aaccattaaa aagaaaaagt ataagaacca tagagattta gcaaaggacg ttatcatgct
     2041 aagtttaacg ggggtgatta cttcgcctat tctttcacag attgatttat ctttaatgga
     2101 ctatctgcaa agaaaaatat cgtcagatat ttttgaaaat catatgtaca gtgatattcg
     2161 tatattggag atattggaag atttaggggt tgtatttttg gttccgcata cggttattat
     2221 aaaatccctg tattctgtct tatcctgttt ggaacaattc aaagaggacg aagaactgaa
     2281 tatgatacaa aatcttttag tgaatggtat tattcagcaa gtagtagcag agtaacaatt
     2341 ttgtttggtt atttgacagg caggtagcca aacaaatttt ttacagttta tatgactata
     2401 tatctataaa cgtcataaag ttaaaggagg aatgaataat gattgatgta aaaaaactgt
     2461 attttagcta tactgacaaa ccgtttgtgg agaatgtaag tttccatgtt gggagaggag
     2521 aaatttttgg ttttttagga ccctctggtg ctggaaaatc aactattcaa aaagttttaa
     2581 ctgggcttaa tacaagatat aaaggcagtg taaaagttgc aggtacagaa ataagggagc
     2641 atacaaatca gttttatgaa aatattgggg tagactttga attttcaacc tgctacgaga
     2701 aattcacagc acgacaaaat ctcgcctatt ttgcttctct atatgaaaag caacctcggt
     2761 ctattgatga attattgcat atggtaggct tggaaaatga cggggacaaa aaagttgctg
     2821 acttttccaa aggtatgcgt tctcgcttga atttcattaa agcgttagtc catgaccctg
     2881 atatattatt ccttgatgaa cctacaagcg gtcttgaccc tacaaatagt cgtttaatga
     2941 aagatattat tcttgcggaa aagaaacgcg gaaaaacgat tatcattact actcacaata
     3001 tgtttgacgc aacagagcta tgtgaccaag tagcctttat cgttgccgga aaagtcagtg
     3061 cattggatag cccgcataat ttgattatgt caaggggagc tgccaaaata caatatacct
     3121 actatgataa aggcgagaaa accggagaat gtctgcttga tagaaccaca gaagataagt
     3181 tgttaaaaag tttaatttcc gaaaaccgtt tgctttctat tcatagtagt gagcctacat
     3241 taaatgatat ttttgttgat attacaggga ggacacttca atgagattgg gacgtttaat
     3301 ttgtggtgat atacatttcc aatggaaata tggtttctat tttatctact ttatattaac
     3361 agttttatat gtgtgcggga ttgccgcttt gccggaacat tggaaaacgg atattgcttc
     3421 tattatgatc tactctgacc cggcagcaat gggattattt tttatgggag cgattgtcct
     3481 cttagaaaaa agtcaaaagg tgttaaatgc tatggtggta tcccctgtaa aaatatcgga
     3541 atatatactt tcaaaaacag ttgcgttgat tgctatttct acggtaatcg cattgatatt
     3601 aggggtagtt tccggcagca atcatttact gggtatcgca gttggtacgg ctttgacctc
     3661 agcaatcttt actatgcttg gaattattgc agctacaaaa atttcaaact tgaaccaatt
     3721 tctcattgtg attatgccga tagaaattat ttgctttgta ccacctattg ttggtctgtt
     3781 tgtgaagtta ccatatctgt tccgcttttt tccattcaca gcttgcatga accttataac
     3841 tggaaaaagt gttttactat cgtttgatat ggtacttgta attgctacgt tgattatcct
     3901 ctacatagtt gctcgacata cagttgagca tatgtggaaa agtttgggag gtgtaaagct
     3961 atgaaaaaga tattatccag caccatacag atatttaagc aaatcaaaag tgaccctatg
     4021 atgtttgcgg cttgcttctc cccttttatt atggggactt taattaagtt tggtattcca
     4081 tttcttgaaa gaataacgaa gttttcttta caagcgtatt atccgatttt tgatttatta
     4141 ctttctatca tggctcctgt attgctctgc tttgcatttg caatgattac attggaggaa
     4201 atcgacgata aagtgtcacg gtacttttcg attacccctc ttggtaagtc ggggtatctc
     4261 tttacaaggt tgggagtacc ctcaattatt tcggcggtca tcgcttttat tgtactgtta
     4321 cttttttcat tagaaaagtt gtccattgga atgacaatat gtttggcgct tctcggctca
     4381 gtacaggcaa ttattgtttc acttatgatt atcacattat caagcaataa attagagggt
     4441 atggcagtta caaaactagc tgcacttaca ttgctcggaa taccagcccc cttttttatt
     4501 gatagttact accagttcgc ggttggcttc cttccatcat tttgggtagc gaaagccatg
     4561 cagaatgaag cggttcttta tttctccata ggattggtgg tagctttagt ctggtactac
     4621 ttccttacaa aacgtctgtt tcggaagctg gcaggataag gaggcatatt atggaaaaag
     4681 gtagacttct gcaaaacaaa aatatgaata aggctattca ggtttccaaa attacactaa
     4741 tcatcaatct gagtttatcg ctacttaaat ttgctgccgg atatatagga aagtcaagtg
     4801 ccatgctgtc ggacgctgta cattcggctt ctgatgtagt aagcacaatc gttgtgatgg
     4861 ttggaattaa aatatcagaa aaacagcctg atagagaaca tccttatggg catgaacgta
     4921 tggaatgtgt ggcttccatt attctctctg ttgcattggc tataacgggt gctggaatag
     4981 gatattcagg tatcaagaaa atcttttcag gacagtacaa tacgctttct gtccctagtg
     5041 gaatcgcact tacggcagca gtcttatcta ttgttataaa agagtggatg tactggttta
     5101 caagaagtgc tgcaaagcat acaaattctg acgcacttat ggctgacgca tggcaccacc
     5161 gctctgatgc actctcgtcg gttggttcct taattggcat tttaggagcg cgtttaggct
     5221 atgctattct cgaccctatt gcaagcgttg tgatttgcgg ttgtatctta aaagctgctt
     5281 tggacatttt caaagaaagc attaacaaaa tggtagacca tagttgtgac aatgctactg
     5341 aaacaaagat tagggaagtt gtgcttcagc agcaaggggt tgatggtatt gatgaattaa
     5401 aaacccgcat gtttggagcg aaaatgtatg tagatattga aatattagct gatggtaatt
     5461 tagctcttta tgacgctcat agaattgctg agggagtcca tcaaacgatt gaaaacaact
     5521 ttccacaatg taagcattgt atggtacatg ttaatacgaa tgaacttctc tattctgcat
     5581 aggacaggga agatgattta aggctattga aagggaaagg agggaagtag gttatggaac
     5641 aatactggtg gattatcatt gttgtggttt tgattgtcgt tggcttactt aaaattgtaa
     5701 ttatgaagaa gtataaccaa aggagaaatt ctaacaatcg ttcaaaagat agcgaggacg
     5761 acaagatgta ttaaggttta ttgaaatctg ttatgttgta gaatgatgtt tttagatact
     5821 agaaagtgct ttaagggggg ctaacgttaa ggaaagcttt gagtacaatt ctttacaatc
     5881 aacagtcttt tgcatagaga aatgtatgta ggaaatatgg tgcaaggcac aaaacaccag
     5941 caattgagaa atcaacctat gaaccaaagg cacactacat tttatactac ttgacaagta
     6001 aacgaacatt tactttaatg gtttaaagaa gtaaacgttc gtttactaaa gaaggaggtt
     6061 gtaaatgtct gacacaaaag aaaatatatt aaaaattgca ctacacttat ttgcagtgaa
     6121 tggttatgag gctgtttctg ttagtatgat tgcggaagcc ttgggaatga caaaaggagc
     6181 attatataaa cattataaaa ataagcaaga tatttttgac tgtatcgtag aacgtatgta
     6241 tcaaattgat aataagcgag ctaaggatta tcaagtacca gaaagcacaa tagaggtaac
     6301 cccaaatgca tacagaaata caactattgg aagtctaata acatttactc ttgcacaata
     6361 cgatttttgg gtgactgatg aatttgcgtc taattttcgc aaaatgttaa tactagagca
     6421 atatagaaat ttaaaaatga atatgcttta tcaaaaatgt cttgtaaatg gtcctatcag
     6481 ttatatagaa gatatattta atgaaatgat tgaaaaaggt gttcttcaag agtgtgatac
     6541 aaaacagctt gctattgaat attatgcgcc attatttcta ttgataagtg tagcagacgc
     6601 agttgaaaat tgttgggatt taaaaactca attaactaca cacgttaaac aatttgtcaa
     6661 gaaatatatt ataaatgaga aagtaaaata atcctttatt ctgtcctaaa tataaaccta
     6721 actttttaat ttcagcagag aaactcaaaa tgaatattgt tataagtcac aaatattaac
     6781 tattaaaata ttacaataac ggagatgaga aaatggaata tattcgcagt aaaaaatatt
     6841 ttacacccga cttacagaca aaaattatgg ggccgaatcc tataaaatta caagaggaac
     6901 tattacaaaa tcataaaatc ccgtctggta gctatgtctg tgaccttgga agtggacaag
     6961 gtttaacttc tgtatttctt gcaaaagaat atggatttgt tgtttttgca gttgacttat
     7021 ggagtgagcc aaaagaaaat gaagaatttt tcaaaaaaat gggattatct tctgcacaaa
     7081 ttaaatctgt aaaagctgac gccaatgaac ttaaatttga gaaagagttt tttgatgctg
     7141 tagtaagtac agattcatat aattattttg gacgtgaccc tatatattta gatgaaaaat
     7201 tgcttccatt tgttaagaaa ggtgggtttg tttatatcac agttcctggc atgaagaaag
     7261 attgccatga gaaacttcca gaagagttat tgttatcatg gacaccagaa gaacttgatt
     7321 atatacatga tataacatat tggtctaaca taattagtca ttgtaaaaag gcagatttaa
     7381 tttcaatata tgaaatggaa agtaattcgg aagtttggtc ggattggata aagcaaaata
     7441 atgaatatgc aataaatgac cgtaaggcaa tagaagctgg cggtggagat tatctgaatt
     7501 ttattgctat aattctacaa aaaaaataaa ttctttagaa ccaaacacta taatttgcgt
     7561 tctaatatgt ttaccgattg acatcaataa ttttatcttt gagggtaatg atttgaaaat
     7621 agaagaatac aagaagatac gaaattgata aattagaatt tgatgggatg gtgatattat
     7681 gaaaatatta tggatcatta ttattatggc agataatctg ctgccgttac tgcttgctcg
     7741 tttctatcca aactataagc ataaagaaat ggcactatct gtccttggct ctcgtcaaag
     7801 cccggtgaaa tggatatata atgtgtggtg cattatatca ggtgtggtat tttgtatcgc
     7861 tccatatgcg ttgtatcaag agaacgatag cggatgggca atcgctatat ggatgcttct
     7921 tgctatatac ggcatcgggt gtgaaatcat ttccggagtg tgcccgttaa atgaaaatag
     7981 acaagacgaa gatataatta caaaaattca cggcggcaca tcggcaatcg gatttatggc
     8041 cttgcttgtc gttcctttgc tctttgcaat tctgcaattc caaacaacaa aagtagtgtt
     8101 aggcgcaatt tcactggtgt gctttacaat agcattcata ttcttctgct tttttattat
     8161 gggtgagaag gaaaagtttg caaatactgt tttgagatat ggcggtcttt ggcaaaggct
     8221 tgttttaata agttgttata taccgttatt tgtttggtct gtttctaatc aataaaattt
     8281 agtttatagg aataacaaat tccagtttgt aaaagtcaat aaatccaaag ataaatttgt
     8341 atttgaaagg agttttgaag aatgaaaaaa tatgagtatg tagaaattaa ctataatgcg
     8401 aaaggagttg tattcctttg tactgataaa cacaaagaaa ttattgacag ttacgcaaaa
     8461 acggggtatc actacgcagg gtttgtaccc acagaaattg acgcaaaagg ctgtatgcga
     8521 aagatagatt taatttttga aaaagaagat taacttattt tagggagttg gttctaatga
     8581 taggacttaa aagacgagat atgaattata ttatgaaagt tggtgctaaa gctcaaggtg
     8641 tttctgtatc tgaatttagg gctatgctac aagataccat agacaaaact atggaaagta
     8701 cagacccaga cgtgcaggca aattttaaac gatactttgg aaataagcgc ccaacaccag
     8761 aggaatatat ttatacgatt accaaaaaga caaaggttaa ggtttaaatg ttgtaaaggg
     8821 ggcttgccgt atgaaacgct ttatgtacaa ttcagtatat tcaagcccaa tttaacagca
     8881 tactatttac aattaaatac gctataccag tagctcgtac aaaatttata ttttgtgcgg
     8941 gcttttttaa tacccgaaaa gtttttttaa ttattttcat ttttttggaa gcaaatcaca
     9001 ccttgctgta cagatatggt gcggaaagag ggataaacac ttttcaattc atagctggaa
     9061 aggaggtgag attatgaaac cgtctgactt ccaaaaaaca attcagtgtc agtttgaatg
     9121 taagctgaaa aaagttgtca aaggtattat ctggaactac cgtaaagaat taaaacgaag
     9181 aaaaaacaag gaaatatctt ttagcgaatt accagacatt acggtagaaa gtctggcaac
     9241 atgggacgac tacgaaacaa actacacaat ttttaatgtg tgtggcatag atattaaagt
     9301 ccttgatgat gaacttgcag aagctctcaa acagctaccc gaaaaaaaac gtaacaatgt
     9361 attgatgtat tacttcttgg aaatgagtga tacagagatt gcagatatta tgaattatac
     9421 cagaggggct gtgttcaaac acagacaccg tgcactagaa actatgaaag agatactaaa
     9481 ggagaagaaa tagaaatgaa gaaaaagtac aagcacccgt cttattattt gatttcatca
     9541 gctatggatg gggatgaaac tgcgattgag aaattactgg cattctatga cccgtacata
     9601 tcaaagtgtt gtttacgtcc tttatatgat gaatacggca atgtttatat tgttgttgat
     9661 atggaattaa agggacgtat tcgagaagcc ttaatcaaaa tgattttaga ctttgagatt
     9721 gatatagata tagaaaccga agaagaataa gcaaatacca gcagggcgtg attggttaaa
     9781 ttcccttctt tcctgccctg ctcctgcttt tatacatgga agcctacggc tttcgccgat
     9841 tgttctttga caactgaata aagcagacag atacgtttgt gatacagcga gccacgggga
     9901 ctgtacgcca tgacctttcc gacaagaaag cgagcgaccc acgcagttga tccgagagcg
     9961 acttgtggaa tggtcgtttt gccatgacct gctatcacag aataatgata cgtccgcatg
    10021 gtgcggttct gcccacaaga atgggaatag ttgagatact aacggagctt tccaaagccg
    10081 tctgtctgct tatgataaga cacacagaaa ggagttgttg caacatgaac aatactgatg
    10141 tgccgatttg ggaaaaatac acccttagta tagaagaagc gtcccgatat tttcgtatcg
    10201 gggaaaagaa gttgcgtaaa cttgccgagg aaaatctgga ctctggttgg gttatcgtta
    10261 atggaaaccg tattcagata aagcgaaaac agtttgaaaa aattattgat acactggacg
    10321 aaatctgatg tcttagagcc gtagatatgg tataatatta gtagcgtatc acggctcatt
    10381 ccatgacgga aaggagcttt acactatgtc aaaaatgaaa agacaagaca gtaaaggacg
    10441 caatttgcgt cttggagaga gccagagaaa agacggaaga tacgtttata aatataccga
    10501 tatattcggg aagccacagt ttgtatatgc ttggaaactt gtaccgacag acaaaacacc
    10561 tactggaaaa cgtgatgata aatcattgag ggaaaaagaa aagcagataa aaaaagacct
    10621 tgacgacggt atcgacacta tcggcggtaa aatgacagtc tgccagcttt atgcaaagaa
    10681 gaacaaccag aaaaagaaca tcaagagaaa cacggaaata ggacgacagt atcttatgaa
    10741 cgcactagaa aatgacccat taggtatgag ggcgattgat acagtcaaac agtccgacgc
    10801 taaagaatgg gctatcagaa tgaatgacaa aggttattcc tataagacaa tagataacta
    10861 taagcgttcc ttaaaggctt cattctatat ggcgatacaa gacgactgta tcagaaaaaa
    10921 tccttttcat ttcaagctaa gtgatgttct ggaagatgat acagagccta aagttatctt
    10981 gacaccagag caggaagaaa aacttctttc ctttatggaa acggataatg tttacagcaa
    11041 gtatcgtgat gaagttattc ttctgctgga aacgggactt cgtatctctg aattatgcgg
    11101 actgacaaca cacattgata tgcttaatcg tgtgattaac atagaccacc agctattacg
    11161 tgatacagag gtgggctact atatttccac acctaagaca aaaaacggca agcgagagct
    11221 tccattgaca gaaagagcat atcaggcact tgaacgaatt ttaaagaacc gaggaaaagc
    11281 tcaaccgctt gttgtagacg gttatagcaa tttcttgttc ttgaaccgtg agggcttgcc
    11341 aaaggtagca ggaaattatg aaagtatggt gcgtgggctg attaagaagt acaacaagac
    11401 ccatgaggac aagttgccga acatcacgcc ccactcattc agacatacct actgtacgaa
    11461 catggcaaat aagggaatga accctaacac cctgcaatac atcatgggac acgcaaacat
    11521 aaccatgaca ttagggtatt atgctcacgg tactttccag tctgcaaagg cagagttgga
    11581 aaggctggct tcttaatatc gaaaatgtat cttactactt atttactact tttggttgca
    11641 ttttcatgct ggtaaatgcc agcttatgcg aggtatcttc caaaaagaaa atgcccatac
    11701 agcccgaaaa tacgggatat atcggcattt accaacttat gaaaagataa tcagaaatta
    11761 gtaagttttt atatcaaaaa aatgattact gtaaagaaat cattttttat tataatcact
    11821 gatacatcaa atattctaaa taaaattttt atttatctgg aattgtatat tttttctata
    11881 atattaacta cagcgtacat tgcccaagca cagatagcca atacaaatac tcccatcatt
    11941 actaaatcta atttaaatac ttgagaacca tatactatca agtagcctat tccatatctt
    12001 gaaactaaaa attctccaac tattacacct acccatgcca taccaatatt aattttagtt
    12061 aaatttatta aatttcctgt gtttgcaggt aatatgagtt taaataatat ttgagattta
    12121 gtagctccaa aactttttaa catcttaatt ttttcttcat ctatattcat aaaataatta
    12181 taagctgaaa gtatagtaac aacaacagaa atcgttacag ctgttacgac gattccttct
    12241 attccagcac ctacccaaac tataattata ggagctaaag cagtcttagg aagagcattt
    12301 aaaactacca aaaatgggtc taatattttt gataattttt ctgaccacca aagagcaatt
    12361 gctaccaaaa tacctaatac agtacctata acaagaccaa gaacagtttc atatacagat
    12421 attccaatat gtttaaataa ttgaccacta aatgcatatt gaataaataa attgtaaata
    12481 tcacttggtt tgctaaatag aaatgtattt ataacattta agtcagctaa aagttcccag
    12541 attacaataa aaccaattaa aattaatagc tggtagaata atacatttct tttttctctt
    12601 tttacacctt taacatagtt tatatatcct tcagatcttt tattaagcct catgtgtatc
    12661 tagctccttc cataatatgt cgaaatactt ttgaaaatca ggaactttac gtgttttgag
    12721 aggcgttttt gttccatcca tgttaagttc aatatcgtaa atatttttta tggtagctgg
    12781 tcttttagat agtattgcaa ccttatctga tatgcttatt gcttctgcta tagctaaaaa
    12841 agtgcaacta attgcttaaa gaaacagaat tatcaacttc agcacccata taaacaatat
    12901 taagatttcc taaactgcta tcccaaacga tactattaat aatagtagat aataacaatc
    12961 tcttatcatc taaactggca ttatcaactt catcattaaa tctcttcaaa ttactaacaa
    13021 taatatcaat attaatactg tcactactaa tattgatgtt actagattcc aaaatattaa
    13081 tcttatcctt taaaatacta atctcattat ttaggctctc aatctgggaa actatataat
    13141 cagaagcagt actaccagta ttttttgcaa gctctataac taaattttta ataagtcttt
    13201 ccttatctaa aatttgattt ttaagtaaat tagtctcaga tttaaaaata ttaaaaacat
    13261 tatcagtctt aatgttatta ctattgaatg cacttataat agactcttct ttacaattct
    13321 taattttatc tataactaaa ctttcaacta atggaccatt aagattttta cagttgcaac
    13381 tatcagaccc aagtgttttt ttagtaccac aaatataata atactttaaa tccttgtcag
    13441 tttttttgcc tttataggta attctcatat tagaaccaca tttaccacat tttataagac
    13501 cagataaaag agcagttttt ccagttgata tccttggagt tttatcacga tttttatcta
    13561 attgattttg tacttcaagc catatttcag gttctatgac tccatggtgt tttgcaattg
    13621 ctgctattga gttagatgta tttttagcat aggtaaggat tccacatttt ctatctggag
    13681 tacctacaac atccatacca ctatttttaa ggtaatctaa aatttcatca ttagctttta
    13741 catatgctgg atttctaagt attctagata aagaactagg gtcaaatttg ccaccacgag
    13801 gaccccttat tccattatta tagacatatt tatataactt agataaggac ctaagttcta
    13861 aatatttatt gtatattagc tttacaatct ccatttgttc atcatttact tgaagcttat
    13921 acatacttct ttcttttaga ttttcatcta agaatgtgat tttttcactg ttgaatccat
    13981 gaggtgtatt gcctccaagc catctaccac tacgggctaa ttcatacata ttgtctttta
    14041 tacgttctgc tattgtttct ctttctaatt gagcaaatac acttgatata aacatcatag
    14101 ctcttcccat tggagttgaa gtatcaaatt gttctttgat agatataaat gatatgctta
    14161 gttcatttaa tttttctatt aaagtagaga agtcagatac atttctcgat atcctatcta
    14221 atctataaca aactaggtaa ctaaatttct tatctttggc atcctgcatc attttcttaa
    14281 attgtggtct atctatattg ctaccactaa atccctcatc ttcatatatc aaaaaatctt
    14341 ttacaccaat agactttgca taatcaatac ataattgtat ttgattttct atagaatcac
    14401 cttttccagt aaatcttgat tttcttgaat aaattgcagc tttcaaaatt aaaaccccct
    14461 ataagttagg atatatattt gaaactattt atctacaacc ttttaagtaa acttattaga
    14521 atgaattt
//
LOCUS       DAEMGG010000102        14299 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEMGG010000102 DAEMGG010000000
VERSION     DAEMGG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.102"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..704
                     /locus_tag="KPK24_002803"
     CDS             <1..704
                     /locus_tag="KPK24_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG2300456.1"
                     /translation="NCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCCMESVGINLIE
                     FLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIYEVRPLNCRMF
                     GHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDRFIPNKDYLSK
                     SKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYNVKIRISKEYK
                     IRKRTIDRIKRIVLV"
     gene            763..1674
                     /locus_tag="KPK24_002804"
     CDS             763..1674
                     /locus_tag="KPK24_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2300457.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKFIEAGKFELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            1725..2537
                     /locus_tag="KPK24_002805"
     CDS             1725..2537
                     /locus_tag="KPK24_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG2300458.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            2646..3338
                     /locus_tag="KPK24_002806"
     CDS             2646..3338
                     /locus_tag="KPK24_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2300459.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            3425..4366
                     /locus_tag="KPK24_002807"
     CDS             3425..4366
                     /locus_tag="KPK24_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300460.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLNQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            4403..5560
                     /locus_tag="KPK24_002808"
     CDS             4403..5560
                     /locus_tag="KPK24_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2300461.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            5712..6272
                     /locus_tag="KPK24_002809"
     CDS             5712..6272
                     /locus_tag="KPK24_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG2300462.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            6300..6791
                     /locus_tag="KPK24_002810"
     CDS             6300..6791
                     /locus_tag="KPK24_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300463.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            7020..7571
                     /locus_tag="KPK24_002811"
     CDS             7020..7571
                     /locus_tag="KPK24_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300464.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            7657..8589
                     /locus_tag="KPK24_002812"
     CDS             7657..8589
                     /locus_tag="KPK24_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG2300465.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            8644..9507
                     /locus_tag="KPK24_002813"
     CDS             8644..9507
                     /locus_tag="KPK24_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG2300466.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            9754..11616
                     /locus_tag="KPK24_002814"
     CDS             9754..11616
                     /locus_tag="KPK24_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2300467.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            11619..13085
                     /locus_tag="KPK24_002815"
     CDS             11619..13085
                     /locus_tag="KPK24_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG2300468.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQRKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            13109..13945
                     /locus_tag="KPK24_002816"
     CDS             13109..13945
                     /locus_tag="KPK24_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2300469.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         5648 a         1369 c         2566 g         4716 t
ORIGIN      
        1 taaattgtat agattattcc attaaaaata agctctttga caaactaaat gatgtatata
       61 agtccttgcc agtaggaaat tgttctggat gtggaaattg ttgtatggag tcagtaggta
      121 taaatttaat tgagtttttg aatatatttt gttatcttga agatagagtt gatttaaaaa
      181 aaagctgtat aagtaaaata ttagattatt attttgagga gtatacaaga aagaacccat
      241 gtccatttaa agatactaat aatagatgtt taatatatga agttagacca ttaaattgta
      301 ggatgtttgg acattggaaa aaagaggatt ataataaaaa tttagataat gttattgaga
      361 aaaataataa ctatagagag ttaatgaaaa agcaatatgg atttgaaata aatgatgaag
      421 tagtaaatta caggatagat tattgtgata gatttattcc aaataaagat tatttaagta
      481 aatctaagag attaagcttt tttgatgagc ttatgatatt agactcaaat atttattcaa
      541 gtggaaatat agatatagac tttagggata gaggtatagt tgaatatttt atagagtcat
      601 tactttatag aaatttagca tataatgtga aaataaggat atctaaagaa tacaaaataa
      661 gaaaaagaac tatagataga ataaaaagga ttgttcttgt atagttttat attagtaagt
      721 aataaattgt atcataaata ttgtcaaaat gaggtgaaga aggtgaatct tacaaaagta
      781 aaaggaaata ccttttttat aaaaggtggc acgaatacag gaatatatgt atttgaagat
      841 aacacagcac ttatgataga cccagggtta tgtggttcaa gacctaggag agtaaataaa
      901 gtattagatg aaaataatat acaggttaga tatattataa atactcatgg tcatgatgac
      961 cactatggag cttgtaatca atttaaagaa tattataaag atgtgtgtat aatgtcatct
     1021 gaatacgata aattatatat agaaaatcca gaattgtttt caaagtatat tgttggggga
     1081 aagagcaatg tatttatgga taatatctta aaaaataaaa tgttggatga tataaaaata
     1141 gataaattta tagaagctgg aaaatttgaa ttaaacaatg aaatgtttga tataattgaa
     1201 tttcagggtc atacacctgg tagtatagct gttttgacga aggataatgt gatttttgtt
     1261 ggcgatttat tgataggaga tgaaatttta tcaaaatacg atttcttgtt tctatatgat
     1321 atacaagaac aaataaattc attagaaaaa ttaaaaaata tagattttga gtacttagtt
     1381 ttagggcatg gcaagcaagt tatatcgaaa gaagattctt ataaattaat agaaaaacat
     1441 ttgaatgctt taaaaaaata tttatatcaa ataagagagt tgcttgtaga accgaaatct
     1501 ttagaaattt tattaaaaaa cattataaat aacaataatt taagcaataa ttataaagag
     1561 tatcattttt ttaaatcttc gttagtatct gtcataagtt acttagttga tttagaagaa
     1621 atagggtata tattggaaaa tggagaattg ctttactata caaaaacaaa ataaattatg
     1681 ataaaataaa gaaggtcata aaaaacaaat aaagggtgag caatatggaa tacgaaaaat
     1741 ggaatgagat actagcacct tatgaacatg cagtagagga attaaaaata aaatttaaaa
     1801 atataagaaa agaatattta gcaaaaggtg aatattctcc catagagttt gtcacaggta
     1861 gaacaaaaaa aatatcttcc ataatatcta agttaaaaag gataaatgca aaagatatag
     1921 aaactgaaat tgatgatatt gctggtataa gaataatgtg ccagtttgtt gaggatatat
     1981 atgctatagt tgatttgata aaagtaagaa atgatatgac tataatagga gaaaaagatt
     2041 atattacaaa ttacaaagat agtggttata gaagctatca tgttataata aaatacccta
     2101 taaactccat tgcgggttca aaggaaatta tatgtgaaat acaaataaga actcttgcaa
     2161 tgaatttctg ggctacaata gaacattcat taaaatataa atatgaccat tacatacctg
     2221 agactcttgc tgaaaggctt agaagagcat ctgatgctgc ctttctttta gaccaagaaa
     2281 tgagtgaaat aagagaggac ataatgaaag ctcaggctat gtaccaaatg aagtctatag
     2341 ctattagaga tactctaaat agaatacaag agttatatga cttgggtgat acacataaag
     2401 ctatgcagta ccaaagaaaa cttgatagaa ctgaaacaga taaaaatata actgaaattt
     2461 tggaattaaa acaagaaata gatatattat tagagcaata taaggatact aaggtagatg
     2521 ataaagtagt tcagtaaaaa ctacttgtag aaaatttgct aaatattaat ggggctgcct
     2581 cttaatggaa aataatttct aaaaagagac agcctattta gtgtaattaa gaaaggaatg
     2641 atattatgag tttgtatgct ataggtgatt tacatttttc aacatcagtc aataagccaa
     2701 tgaatatctt tggaagtaat tgggatggtc atgaaaagaa aattatagat aattggaaag
     2761 aagttgttaa ggaagaagat atggtcttag tactaggaga tacatcttgg ggaattaatt
     2821 tatccgaagc taaaaaagat ttagatataa taagcaaact tccaggtcag aaaatactta
     2881 taaagggaaa tcatgattac tggtggacaa ccgttacaag tttaaataag ctatatgaag
     2941 acatgcgttt tattcagaca aatttttatg aatataaaga ttatgcaatt tgtggaggta
     3001 ggggctggat atgtcctaat gatgttaaat ttgatgagac tgatgaaaaa gtttataaaa
     3061 gagaagaaca tagacttaga ctatcattag aatctgctcg taaaagtggt cactcaaaaa
     3121 taattgtgat tactcattat cctcctacga atgataaatt ggaagaatct ttatttacaa
     3181 agttatttga agaatataat gttgaaaagg tgatttatgg tcacttgcat ggtaaagaat
     3241 cttttaaaat gggattgaag gggattcgaa atggagtaga atatacatta gcatcttgtg
     3301 actatacaga atttaattta ataaaagttc acgattaaaa aacataaatt ggtaaatact
     3361 ataagataca tgattaaaaa taaatatttg agggatttcg gttttgagaa aggagacacg
     3421 acttatgagg tggatattag tttttatatt tttatatctt attcctttag tagtgatatt
     3481 cagaaattac aagaatttta agaggtcttg tatctacagt agtatttatg tggtattagc
     3541 tacaacaata atgataagta atatttatat gagtggactt aataaaataa aagagtctat
     3601 gtattatcac aattatattt ctgatgagac atatgatgaa aagtatgtat caaattttaa
     3661 ggaaaaagaa agtactgatt taaaccagga taataaaaag aatgaagaaa tatcaaataa
     3721 taaaaattta aacaacaaac aagatagaaa tatacaaaat aaagaaagta atgaagaagg
     3781 aagtaatgat ataaaagaaa agaataattc tgatagtaat gtttcagtga ataataaatc
     3841 agaaaaacaa gaagataata caaaagaaaa taataaatat acaagtaaag aaaaaaatag
     3901 tgaagatata aaaaaaatag atatggaaag tataggaatt tttaagaaag aaatttataa
     3961 tatagaaaga gcagctttag ttccaatgag agaatgcata ccttacacta aggatatagc
     4021 aaaaaatata actaaattat ctagtataaa aaaagatgtt acaaatgcca gaaacaagtg
     4081 taaagaagtt gtaaaagaat atgaaaatat gcagattcca gaactttcta aggagaagta
     4141 tacagaaaca ttgagtaatg ctagagatga cgtaaagaaa gcatatgagc ttagagaaaa
     4201 atctatggaa agtgctttga aattaataaa tactaaaagc cctaaatata tagggaagat
     4261 aacagaatat cttgatttat cagataagca gatagaaagt tttaaaaata gattgaacga
     4321 tttaaaagaa gaaataaata attcagaaac aaataatatt aattaataag atttaaggat
     4381 ataagttaaa agggagatta tgatgaataa acagagtata ggaaaactaa atatgttttt
     4441 tatggggatt actcaaagaa accttgataa tagggatttt tttattgagt tagaggtgat
     4501 ttttaaatct ggaagaaaag agtttaaagg aattgcaata gaggaagata ataaatataa
     4561 atttaatttt aaaggaaaat cagatttata tacgtttaat gagttgctta agaatatttc
     4621 taaagaggca gaaaattatg atggattagt atttaaatat gttgagcgag gaactattgt
     4681 tgtaattgaa ggagacaata agaaggtaaa tgtaaaatat ttagataata aggaagaagt
     4741 acctaaaata gatgaattta cggcatctca aataaaaaac agagattatt atgtaaaagt
     4801 aggacaagca aatgcacttt taaaagaaat tggagtactt acaaaagatg gtaagataaa
     4861 aaatgataaa ataagaaaat acaatcagat agaccatttt gttgaattga tagatagtat
     4921 tttaaaagaa ataaaggata aagactgtat aactatcttg gattgtgctt gtggaaaatc
     4981 ctacttaagc tttgtcttaa atttttatat aaaagaagta ttgaagaaaa actgttattt
     5041 tataggaata gattattcag atgtagttat agaagcatca aaaaatatgg ctaaaaactt
     5101 gggttataaa aatatgtcat ttataaaaga ggatttgact aattatacac ctaataggga
     5161 tgttgacctt gtaataagtc tacatgcttg tgatacggct acagatatgg caataggtct
     5221 aggaattaga gcaaaatctg aagctatagt agtagtacca tgttgtcata aggagctatt
     5281 agggcaatat agatatgaag ctatggaacc aatattaaaa catggtgtat ttaaagctag
     5341 atttgcagat ttaataactg atggtttaag aacattgtta ttggagggca atggatatga
     5401 tacttctgta gtagaataca tatctccact tgatacacca aaaaatttaa tgataagagc
     5461 gataaaaact aaaacgaata atgacaaagc attaaaagaa tataaagaac tgaaatcaca
     5521 atttggagtg gaaccaacct tagaaaaact tatatattag ttaaaaatgt aaaatttacg
     5581 aaaagcttgc ttaaatatgc aagctttttg gtattatata ggtatagtca tatccagagt
     5641 tttgatttat aattaaagct gtgctataat atttgattaa tcaaatcaat aaatgttaca
     5701 tggaggtaaa aatgaacaaa ttgaatatat gtattgatat agatggaaca ataacaagcc
     5761 cttatcattt tcttccatac ctaaatgata tctttaataa aaatataact gaagcagagt
     5821 gtataacaca taattgggaa gaattgtatg gttcgggaat aagagatgtg tatgcggagt
     5881 ttaataataa gtatatacac tcatatgatg aagcaaagat tgtagagggt gctacagagg
     5941 taattagcac attatctaaa ggacataatt tatcttttgt aacggctaga catgagtgtt
     6001 taactgatgt aactaaaaat tggctatcga gacaaggatt ttcagatata gatgtttact
     6061 tattaggaag tgattataaa gttgaaaagg caagagaact gagttgtgac atatttatag
     6121 aagataatcc tttaaactca gtgcaactag ctgatgatgg tgttaaagta atacttctgg
     6181 atactaacta taataaagat gttaaacatc aaaatatagt tagagttagt aattggatag
     6241 atattgaaaa gattataaaa caaggtattt aaagatttgt agattgaaag ggggagcata
     6301 tgatagattt aaaaattgaa aatagaaaag gaaaaatcca tgtaaagagc agtgaaataa
     6361 aagacatttt agaattacgt ccagacttcg aatatgtaca agatatatca aatactataa
     6421 atcaagaaaa tatattggta tttgattgcc agttgacagg aaattctttt gatatggatg
     6481 atttagatat agaagaaata ttggaagagt taggtgaaga aatagatgag tcttatttca
     6541 gtgttttatt tgaagatgta agagcgtatt taaaagatgc tactgatgag atagaagcag
     6601 acttacagga taattactta tttgataatt tgagatgtta ttttgatata tacaatataa
     6661 atcaagaatt tacagatttt aagtttgtat ttttagtttc ttttaaagat ataaaaattt
     6721 cttcattagc aaacctagct aagattgttt ctaagagaca gcttataggt gcatctaagt
     6781 tttatagtta aaatgaaaga ttattataat aaaagctagt aaccttgtaa aaatataggt
     6841 tactagtttt ttatttattc attttcacaa aatttcagaa aactatttac attctaaaaa
     6901 aagtaatgta tattaaacat aatgtacatt ataatggata aaaagtttct attaaattac
     6961 taagtttatt taataatcaa aataatttat cttatattag tatgtattag ggggtattta
     7021 tgcaaagttt aaatgaaata attgctgaaa atttaaaaaa gataaggaaa gaaaaacatc
     7081 ttagtttaga caagattgca caacttagtg gtgtaagtaa gagtatgcta tctcaaattg
     7141 aaaagtgtga ggtaaatcct actatatcta ctttgaagaa aataactaat gggctaaaaa
     7201 tctcatttac atcattgatg gagaggcagg aaagtgacat agaattgatt caaaaaagtg
     7261 atatagacca ttttattgaa gataatggaa agtatataag ttatccaata tttccatttg
     7321 actccaaaag aagatttgag atatttatga ttgaaataga agaaggtgga aatttagatt
     7381 caaatgctga acttccagga acacaagagt ttataactgt ttttagtggc gaagtaacca
     7441 ttaaaataaa tggagaagat tacattgtat ctagtggaaa ttctataaga tttaaagctg
     7501 atgtatcaca tatatataga aactcaggca aaggtatagc taaaatgagt atggtagtat
     7561 attatatata aataatttaa tggaattaga gtgatattta gatgatttta ggtatgaaga
     7621 tatattatat ttaagttata ggagtgtgat ttatggatga ataataaaaa agcagttttt
     7681 ttcttttgca gtgatttaaa gaaagaccca gtagccagta atgtgataaa atgttgtgaa
     7741 gaaataatgg atttaaatca aactgatatt gttattgatg ataattttgt attagagttt
     7801 aaggataaaa acaataactt atttcatttt gtaaaaacaa aagatgtaat aagtcataat
     7861 tataaatatt atcttccaat tttaaataga cattttagag attatgattt tgcaggagtt
     7921 gtaaattggc atgagggaga aaatgctcca gaacatatcc taacagctca tacaattgga
     7981 gatgttccaa caggagaatt tggaaattca aatccaagat actttaaaaa tctaatcaat
     8041 gctattgagg atataaaaaa tgaaaatcta cttgatgaat ttactacatt aacagaagca
     8101 actcactggt ctggaacaac atatggtgaa gaatctaaat taattacaga atattctgta
     8161 cctatgttgg atattgagat aggaagtagt agtgatagtt ttaataatag tatagcaata
     8221 caggtattgg caaaatcatt aattagagta tttgattgtg atgaaccttt gaagactttg
     8281 ttatgtgttg gaggggtaca ttttgagaaa tctttctcag atattataaa aaataaagaa
     8341 tataacatat caattggaca tgtattgcca aatcaatgga ttgtaagtgg aatgtatgat
     8401 gatgaatctg gatttgagaa actagaaaag tgtataaata gtattgaagg aggaattgat
     8461 tgtatagttt ttcatgataa attaaaaggt acctataaag agcaatgtcg taaactaggt
     8521 gaaaaactta atgtacctgt atttaaacat aaaatattaa aaaatccaaa agacttacct
     8581 atatggtagt tttatacagt taatataaca ataagtcgga ttaattttta agcgaggtca
     8641 aaaatgatat tatcttattt tgcattatta attagagtag tgctattagc ttttgaaaga
     8701 gtagttgtaa gattattagg tgatgaagaa ggagatattt ataaaaatat agcttcatca
     8761 tttctatttt tctttatagg gggagtatgt ttacttccat ttagcataat ggatagagtt
     8821 agtgattggt cgtttttaat accatgttat ataagtagtt tagtctattc aattggttca
     8881 gtagcgtatg taacgtcttt agcaactggg gaagtttcac ttgttactcc aataaatagt
     8941 ttaaattcat tgtttttact gcttttatca gttatatttt taggtgaagg tttaagttta
     9001 gcaaaagttg caggaatcat aattatgatt ggtggagtgt ttatattaaa aaaagtaagc
     9061 tcttttttaa agaatgtctc tgtaattttt aacaatttac catgtagatt aatgttttta
     9121 tacatatttt tgcagtcagt aggaagagta ttggataagg ctttttttgt acaagtatct
     9181 cctatattat attcaactat actgtatttt ttcgtaggtt taaatttatt tatcttttta
     9241 gcatttaaaa agaaacaaaa aataatttgt gagattttta gtaataaaaa ggggctttca
     9301 attttaagtg gtgctattaa tggatattct tatttagcat tattaattgc tttaaataat
     9361 ctagaactta gtatagcaga gccattttct caagtatcta tgataataac tttaatactt
     9421 gctcatttta tattcaaaga aaatataaaa gaaaaaatac caggttcaat tttgatacta
     9481 attggtgggt ggctactgtt actttaattg atataaaaaa tgaattataa aggtgctatt
     9541 agttagtatc agaaaattta aaaaagtatt gacaattctt ttaggcaaat gttaatataa
     9601 taatacaaaa tagtataaat agaagacatg ttactggtaa tgcaggcagg atcgaatata
     9661 ttgctttgat ttttcaaggt gtttatatag gttctgcctt ttttgcatct tgaatggcca
     9721 ttcataaaaa attatggaga aggagattac aagatgaatt ataaaaaaac tgccattgac
     9781 attattgaac aagttggtgg ttcaaaaaat attttagctt tagaacactg ttcaactaga
     9841 ctacgcttta agttgattga tagaagtaaa gtcaatcagg atgctttaaa aaaagtacaa
     9901 ggtgttatga gtatagttaa tggggcacaa tttcaagttg tcattggaaa tagtgttgtt
     9961 gatgtgtatg atgaaatttt aaaagtttgc cctttagata caaatgatag tactgtaaaa
    10021 actgaagaaa aaataaactt aggaacaaga tttatagaat ttattattaa tatttttcaa
    10081 ccactgattt ttgctattgc aggagctggt attttaaaat cactgttgat gttattagca
    10141 atgtttaata ttttaaaatc agattctact atatatacat tattagttgc tatttcagat
    10201 gcaacattct atttcctacc tcttatggta gctgtaacta ctgccaatgt attgaaatgt
    10261 aatcgtttag tagctattgc cgctgtagga tacttattgc ttccagcaac aacaactgct
    10321 ttaagtgaag gtgtcgaact atttggattt acaattccaa acattgccta taatgcacag
    10381 gttttcccgg ctatcttatg tgtgtctttc ttggcaacaa tggagaaaat ttttaacaag
    10441 tattcaccaa aaccaattcg aactttcttt gttcctatga tgtctttggc aattactgtt
    10501 ccaattactc taactatttt aggtccttta ggatataacg ttggtacaat ttttacaacc
    10561 attattttat tcttatataa caaaatggga ttcttagttg taggattact tgctgctttt
    10621 cttccattta tggttgcaac aggaatgcac aaagcactaa taccttattc aattaataca
    10681 attggaaagt tgggttatga agctttatat atgacatctt cattagccca caatatttct
    10741 gaaagtggtg cttgttttgc agtagccttg cgaacaaaag atgaaacatt aaaacaaaca
    10801 gcattatctg caggaatttc tgccttgatg ggaattacag agccagcttt atatggtatt
    10861 actttacaac ataaaagagc aatgttaggt gttgtattct caagtgcaat ttctggtaca
    10921 tttttaggat tatttgcagt aaaaggattt gtaattgttg gaccagggct tgccagtatg
    10981 agtatgttta ttgacccaaa caatggaaac aatttgattt ttgcaagtat tggttttgtt
    11041 attgcaatat taggctcgtt tattattaca ctgattattt ggaaggaaga tacaaataca
    11101 gtagagtcat caattggaga tgttgttgaa gaagagctag aagaagaaaa agaagtaaat
    11161 atcttagtaa ctccagtaga aggaaaagta atagatttaa gtaaggttaa tgatgaacta
    11221 tttgcaagta agactttggg agatggagtt gcaattatac cgaccaatgg aaatttatac
    11281 gcaccttgtg attcagaagt tgtgatgtta tttgaaacaa aacatgcaat tggtttaaga
    11341 acaaaaaatg gggctgagat tttgattcat attggaataa atactgtaag tatgaatgga
    11401 gatggattta aaacatttgt taagacaggc gataatgtaa aagaaggaga tttattaatt
    11461 cagtttgatt tagacaaaat ctcacatgct aatttagatt caactgttat gattgtaaac
    11521 aataatggag cagattatgc ttataaagtg ttaaaccagt cttatggcaa tgtaaaaaaa
    11581 ggaagtatct tgtttgatgt aaagggagga atataaatat gagaaagttt ccagaaggat
    11641 ttctttgggg aggagctaca gctgctaatc aatttgaagg tggttggaat ttaggtggta
    11701 aagggtggtc tgtatcagat gtagcaaagg cacactttga tgcagatgtg aaagattata
    11761 aatctaataa tgaaatcaca acaaaagata tagaagaagg attagcacat cctgaagatg
    11821 aagtaaatta tccaaaacgt catggtagtg atttttatca tcactataaa gaagatattg
    11881 ctctaatggc agaaatggga tttaagacat atcgaatgag tattgcttgg tctcgtattt
    11941 ttccaaatgg agatgataaa gagccaaatg aagaaggttt acagttttat gatgatgtat
    12001 ttgatgagtt gataagttat ggaattgaac cacttgttac tatgtcacat tatgaaccac
    12061 cattaaatat cgttttaaac tatgatggat ggtattcacg acaagttatc aatatgtttg
    12121 ttaggtatgt agaaactatc tgtgaaagat ataaaaacaa agtgaaatat tggcttacat
    12181 ttaatgaagt tgattctatg attcgtcatc cgtacactac tggtggttta gttcgagacc
    12241 gttttaagga taaaaacttt gaggaagtta tttttcaagc tatgcatcat caatttgtag
    12301 ctagtgccct tgcaacaaaa atttgtcatg aaattattcc aaattcaaaa gtagggtgta
    12361 tgttaacaaa attaacgtat tatccataca catgtagacc agaagatgtt ttggcaactc
    12421 aacgaaaaat gagaagtatt tatgcatata gtgatacgca ggtttttggt gagtatccag
    12481 tatatttact gtcgtatttt aaaaataata acattcaaat agtaaaagaa gaacatgatg
    12541 atgaaattat gaaaaagtat ccagtagatt ttatttcatt ctcttactat atgtcttctt
    12601 gtgaggcagc tgatacaact ggattagata ttacacctgg gaatacgtta ttagctgtaa
    12661 aaaatcctta tttagaaatg agtgaatggg gttggcaaat tgattcaata ggtctacgta
    12721 tatctttgat tgaattatat gatagataca gaaagccttt atttatagtt gaaaatggat
    12781 tgggagccaa agatatttta actgaagata aaaaagttca tgaccaatat agaattaatt
    12841 atttaaaaga acactttaaa tgtatgttag atgcgattat tgaagatggt gttgaacttt
    12901 ggggatatac ttcatggggt tgtattgatt tggtcagtga atcaacgaaa caaatgtcaa
    12961 aacgttatgg atatatttat gtggatgctg atgattatgg aaaggggaca tataatcgat
    13021 ataagaaaga tagtttttat tggtataaaa aggtaattga aagtaatagt atagatttta
    13081 aatgaccaat agaaggtgag caaataaaat gaactacata ataaagaaag ttttgaactc
    13141 gagcgttgtt ttagttcatg acacaaagga taatgaattt attttgctag ggaaaggaat
    13201 tggatatggt aaaaaaacag gagaatatat ttgtggttcc aatgataatc aaatgtttgt
    13261 ccctgttgaa aatacaaaat caaaacaatt tttagaatta atggatgata ttcctgtcga
    13321 cattttaaag ataacacagg aaataatagt agaggcagaa aagttattga actcatcttt
    13381 taataaaaac ttatacttaa tattagcaga ccattttaac tttgcaattg aaaggatgcg
    13441 taaaggaata aaaattacaa atcgagtatt ttgggaaata aaaaattatt atccaaatga
    13501 gtttaaggtt ggaatggttg caatttatat ggtagaggag agattgggca ttcaattacc
    13561 agaggaagaa gcagctaaca ttgctttcca ttttgcaaat gctatggctt cagatggaaa
    13621 ttcatatgat acgattaagt atgccaaagt aattggagaa ataattaata tttttgtttt
    13681 ttctttgaat agaaccttag ataaaaagag tatgcattat atgagattta taacacatat
    13741 aaaatttttt gtggaaagat ttttttcaga tagtatgtta agcagtggtg atgatttatt
    13801 atttaatcaa atgaaaaggt caaatccaaa agaaatgtta attgcattaa aagttagaga
    13861 ttttttagaa aagaaatatg gaaaaaagtt aacaaatgag gaaatagctt tcttggttgt
    13921 ccatatcgca cgagttaagc aatagagtga attactaata ttatcttata cgtctacatg
    13981 gtagaaaatt gaaagaaggt aaagtatata aagaaactga gacatattat tttgttgttc
    14041 attatgttta ttttatgagt gtatcctgtt aaaaatttaa gttaatattt ataattaaaa
    14101 tatacaaata gttaaaataa gtttatgtgg ttatgaattt attttaacta tttttttgtt
    14161 taatggaaaa tatgttttat aactagaatt ttatttttaa ttaattataa tacaaaaaac
    14221 tttataaaat cttcagaatt tcttaaagaa tatgtaagta acataagata tattatattt
    14281 agttggtaat ataaagggg
//
LOCUS       DAEMGG010000103        13756 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEMGG010000103 DAEMGG010000000
VERSION     DAEMGG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.104"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            122..1915
                     /locus_tag="KPK24_002817"
     CDS             122..1915
                     /locus_tag="KPK24_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2300470.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(1996..3378)
                     /locus_tag="KPK24_002818"
     CDS             complement(1996..3378)
                     /locus_tag="KPK24_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG2300471.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            3636..3983
                     /locus_tag="KPK24_002819"
     CDS             3636..3983
                     /locus_tag="KPK24_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG2300472.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            4001..5035
                     /locus_tag="KPK24_002820"
     CDS             4001..5035
                     /locus_tag="KPK24_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300473.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(5084..6133)
                     /locus_tag="KPK24_002821"
     CDS             complement(5084..6133)
                     /locus_tag="KPK24_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2300474.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            6408..7943
                     /locus_tag="KPK24_002822"
     CDS             6408..7943
                     /locus_tag="KPK24_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300475.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPHGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(8128..8814)
                     /locus_tag="KPK24_002823"
     CDS             complement(8128..8814)
                     /locus_tag="KPK24_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG2300476.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            8939..10321
                     /locus_tag="KPK24_002824"
     CDS             8939..10321
                     /locus_tag="KPK24_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2300477.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            10723..11991
                     /gene="eam"
                     /locus_tag="KPK24_002825"
     CDS             10723..11991
                     /gene="eam"
                     /locus_tag="KPK24_002825"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG2300478.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(12084..12440)
                     /locus_tag="KPK24_002826"
     CDS             complement(12084..12440)
                     /locus_tag="KPK24_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300479.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(12591..13685)
                     /locus_tag="KPK24_002827"
     CDS             complement(12591..13685)
                     /locus_tag="KPK24_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2300480.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5389 a         1728 c         2178 g         4461 t
ORIGIN      
        1 tataattgtg ataattagag tgtataagat ttatataaat tatacaaaaa atatttatgt
       61 aataaacttt acatacttat atatttagtt attcaaacta ttatttcgag gaggtcttgt
      121 tgtgaatata aaagatagat taagtggatt aagaaaattt atggaagaaa aaaatataga
      181 tgcctatatg attccatctt ctgataatca ccaaagtgaa tatgtaggag attacttcaa
      241 atcaagagaa tttatatcag ggtttaatgg ttcagcaggg acggttattg tgactaaaga
      301 cgaagctggg ttatggacag atggaagata ttttatacag gcagaatctc aattagaagg
      361 aagtacaata aaattattca aaatgggaca agaaggttgc ccaactacag atgaatattt
      421 atataaaaat attcctgaag gaggaacttt aggatttgat ggtagagtta tatctgctag
      481 agaaggagct acattagctg aaaaattatc taaaaaaggt ataaagatag aatatcagta
      541 tgatttaata gatagtatat ggccagatag acctgcttta tcagatagta aagcattttt
      601 attagatgtt aaatattgtg gagaaagttt tagctcaaaa ttagctagat taagagaaaa
      661 aatgagtgaa aaaggtactt ctactcatgt aataactact cttgatgata tagcatggtt
      721 attcaatata agaggtggag atgttaaata taatccagtt gtactttctt atgcagtaat
      781 aactttaaaa gaagtttatc tatttgttga tgaaagtaag ttaaatgaag aaatactaaa
      841 tgaactagct aaggaaaatg ttcaaataaa gccatataat gatgtgtatg aatttgtaaa
      901 aaatatagac aaaactgaaa aagttttatt agatggaact aagcttagtt atacaattta
      961 caataatata ccttgtgaag ttgagaaagt tgatgagttc aatccagtaa tgttttttaa
     1021 agctcaaaaa aatgaggtag aattagaaaa tataagaaat tcacatgtaa aagatggtgt
     1081 agctttcact aaatttatgt actggttaaa gaaaaatgta ggtaagatgg aaatcactga
     1141 aataagtgct actcaaaaat tagaggactt aagaagagag caagaaggtt tctttgagcc
     1201 aagtttcaat acaattgctg catataaaga gcatgctgca atgatgcact atagtgcaac
     1261 tccagagtca aattataaat tagaggcaga aggattattt ttagtagatt caggtggaca
     1321 atattatgat ggaactactg atataactag gactacagtt ttaggaccta taagtgatga
     1381 attaaaacta cactttactt ctgtagcaag aggaatgata aatctttcta aggctaagtt
     1441 cttgcatggt tgtagaggat ataacttaga catactatct agaagttgca tgtggaatat
     1501 gggaattgat tatcaatgtg gaactggaca tggtatagga tttgtgctaa atgttcacga
     1561 agctccaaat ggatttagat ggagagtagt acctgaaaga tttgatagtg cagttcttga
     1621 agaaggaatg gtaactacta atgaaccagg tatatatata gaaggttctc atggtataag
     1681 aacagagaat gaaattgtag ttagaaaagc tgaaaagaat ttctatggtc aatttatgga
     1741 atttgaagtt gttacgttag ctcctattga cttagatggt atagtaccag aattaatgaa
     1801 taaggatgaa aaagattatt taaactggta tcataagtta gtttatgata aaatatcacc
     1861 tttcttaact gatgaagaaa gagaatggtt aaaagtttac actagagcta tatagtttgt
     1921 actagataca ataaaaatcg aggtattata atgcctcgat tttttattgt atattttatt
     1981 aactttaaaa aatttttaat ttgataattc tactaatttt ttattcatta taagtagtat
     2041 aattccaaat atcaataaaa atgatggtat cactatgaag acttgtaaca taccaagttg
     2101 gtctataata gcttgcgtat agccagataa tttatttgct gcaaatttgc ctaaaaacca
     2161 aacacccatt aggaatgaaa tatacttttc aggcgaaagt ttatttacca taccataccc
     2221 gactggagaa aagcaaattt ctcctatact ttggaatgtt aaaaatccaa ctacccaaaa
     2281 tacacttgct ttgtgaattg aattagggtc tgttcctgtt tgtataactg ctactatcat
     2341 aaacaaaaat gctatggcta atagtataaa acctaagctc attttttttg gcacacttaa
     2401 atcgcctttt ttagtttgag ataattttac ccatactaaa gcagagatag gtccaagaat
     2461 aacacaaagg agaccattgt atgaatctat ccatgaagta ggaatttgaa aattgccaac
     2521 agtaagattt atgtgttcct ttgtatagag tgcaatagac atagaggctt ggttatatgc
     2581 tatccaaaaa aatactgtga aaaaagctaa caccaatatt actattattc tttttttctc
     2641 aatcaaagtt aaaggtcttg aagctagcat tttcttatat tctagtgatt ttcttataga
     2701 ctctctcccg ttactagggt cattattcaa aggtttataa aactttctgt ttccaaatat
     2761 aaagaagaat aaacctatta gaaccgtaat tgatgaaaat aaaaatacat atctatatcc
     2821 atacatactt atagataatc cgcttttcat agcaaaccat tcattagcaa ctaaaccgca
     2881 tattaaaggg ccaaataagg tacctagatt agtagccata tacgtaatag agaaagctcc
     2941 atcttttctt gggtcatcat tggtatataa gtttccaacc atagtatgta agtttccttt
     3001 ataaaaacct attcctaatc cagataaaaa tatcatgatg tttatatcca tcttactatt
     3061 tgctatgtat cctagaaaat aaccaattga aaccattagc attccaagta taatacagtt
     3121 tttgggacct aaaaatctat cactgaccca tccaccaata actggagaga aataagtcca
     3181 tgctaaaaaa gatgcaagca ttgatgcgga ttctgctggt gttaacccta aaccaccttt
     3241 ggagacttgt tcagctaaaa acaataataa taccaatttc ataccatagt atgcaagtcc
     3301 tacaaaggta aatattgaac agcagaaata taaatctcta ggttgtttaa gtttttcttg
     3361 cgtttttaaa actgacatat gtcgagaacc tccagataaa tagtttaaca aaattttacc
     3421 atactatatg agtatatgtt tttttcatat agacgtcaac ttgtaagata atttaattaa
     3481 gtcgaaatat atgtaaaaat agtatcattt ttgtgtattc agtaatattt ttttatgata
     3541 atatatgtta ttattatatt aaaatacaaa catatttatg gaaaggatat gccaatttag
     3601 tagttagatt gattgtttgg caggtgggat gagctatgat aagaaagaaa tctgaattac
     3661 aagtagaagt aaaagaaaat ttaaatggtg gtataggaaa ggttaagctt gaaaacataa
     3721 tacaaaatga agagcttaaa ggaaaaggaa gactatttaa aagagtagct ttaccagtag
     3781 gaagttcaat tggtgtgcat gaccatacaa cagattttga agtatattat atactaaagg
     3841 gaaaaggtaa agtatttgat aatggggaat ttgtagaagt aaatgagggt gatgtagtgt
     3901 atacagctga tggcgaaaaa cattctatag aaaatatagg agaagaagat cttgagtttg
     3961 tagcactagt tttatatatg taaaatatgg aggaggagtc atgacaggca agatagaaga
     4021 aattaaggac aagataaata gattttgcaa ctccaattta aatcaagaat acaaagatat
     4081 aagctttaaa attttgcaaa accttttaga taataataaa gagataattc attcttctag
     4141 agctgatata tggtcttcag ctatactaaa tatagtttta gaacaaaatc ttttatacaa
     4201 taagaagcat ccactacata taacaaaaaa agagttttca aaacaaatag gtgtttcttt
     4261 aaatactata aataataaat cgtctttaat aaaagatgtt tgtagtattg actttttaaa
     4321 tcaagatact atagctattt ctaatgtaga attttgggta aaagagacca attccaaatc
     4381 aaagtctgta gatttagatt tagaaaagaa aaaaatacta tataaaagat atattaagct
     4441 atcacaagat gcgaatagtg atattgaatc aattagatat atggaggaag ctgtaaatat
     4501 aggtaaaagt atgattacta aagaagataa agaacttgga ttttggaaag gtttatctac
     4561 cagagcctat atgatagcac ttgaatccct tgctagaaag ttagaaagta ttaataatct
     4621 aaaggagtcc aaaaaagtat tagaatattt aatagaaatt aatcctagtg atgagcaggg
     4681 aataagatac aaattattaa atgtattaat tagattaaat gatagaactt caataaataa
     4741 attatttgaa ctttataaag aagaaaaatc tgctacatgg atgtacagta aagctcttta
     4801 ttactttaaa aataaaagta tgtttttggc aaatgattct attaaggttg ctagaagtaa
     4861 aaataagtac gttggattat atttgttaga ttggcgaaat gcttttggta gagaatttgt
     4921 tacagaggaa gaaaaggcag aagctgtcta ttactatgat gaaaacatag tgatttggaa
     4981 tgaagttaaa ggttctatgg attggctatt aaaaaaaatg ttggaatttt cataaaaaaa
     5041 actactcctt acttttatat aagtaaaagg agtagttttt aatttataca tctagtttta
     5101 aatcttcagg ttttattaga cctttttttc tcataaattc tgaaatcaac aaagttacaa
     5161 tagcaggaag tataaaatta agtaaaagta taccaaccca catagatata cctccttttc
     5221 cagcactttg catagcagtt actgttccaa attgtccaac caatccacaa gtacccatac
     5281 cagaaccaat aggaatattc tccattttga atattgttgt agataatggt ccaagtaata
     5341 ttgaagctac agtaggtggt atccaaattt tccaattttt tattatatta ggtacttgca
     5401 acatagatgt cccaagacct tgtgccacaa ggccgccaac tccattttct ctaaagctca
     5461 taacagcaaa gcctaccatt tggcaacaac aacctacagt tgctgcacca ccagcaagac
     5521 cacctaaact taacatcata caaatagctg cactacttat aggtaatgta agagctattc
     5581 caacaagtgc agagactaaa gctcccatta ttataggttg catagttgta gcttgcatta
     5641 ttatatttcc aaatgaagtc ataaaagcag aaacagctgg accaataagt tcagctacaa
     5701 gtactccaat tagtattgtt acagcaggtg taaccaatat atctattttt gtttcttttg
     5761 aaactaattt tccaacctct acagcaaaaa gtgtagcgac aaatgctcca acaggtcctc
     5821 ctaattcatt accacaagct ccaacaagtg cacaagaaaa tagtacaagt ggaggtgctt
     5881 gaagtccata cgcaatagcc actgctatag ctggaccagt aacctgttta gcaagagggc
     5941 ttataacttc agtaaaaaga gaaatattga aattatctcc taatgtattg aaaattgttc
     6001 caattaaaag ggacgaaaaa agaccgagtg ccatataact tagtgcgtca ataagatacc
     6061 tttgaacaga aatttcaata ttttttcttt tagcaaatga tttgaaattt ctttttttat
     6121 aattgatttc caatttaaaa tcctcctcat ataatataag tataatttct ttttaattaa
     6181 ttatataact tttcagctga caagacaaat gttatttcac taaaattaga ctattgtaat
     6241 agtaagaaat taacattaat tttagtttta aattttaaat ttatggttta taattagtat
     6301 agggatattc tgaaatataa taattttcca gtttatccca atatttaact taggtagatt
     6361 gcttacaaaa caaatgatta tatattaggc taaaaggaag tggatagatg atatttgtaa
     6421 aaaataagaa atacagaaaa attgccataa ttatatctat tcctatattg cttgttgcgc
     6481 agtactacct ttttaaattt ggaatactta gaccaatggt aaagggagta gaaattgaaa
     6541 ttgtagatgg ggaatatgtt aaagatatag ataaatatgt agtaaaatta aatgaaacag
     6601 ttactttgtc tacaggtgaa tatattaagg ttccttcgta tgctaaaaat ccaaatattt
     6661 ggtttaatgt attggataac tcagggactt taaaaataga tggaaataaa atgacagcat
     6721 taaaggttgg gtattcttct gtgggtatca tgaaaaattc aagagtatta aaaaaggcta
     6781 ctataaaagt agtagaccca gaggtagaat cactagatat ggagattaat ggggatttaa
     6841 aacatgttgg tgatagtgct actatagaaa gtacaataga agttgattat gataagttta
     6901 aaaaatcata taagccagtt tataagagta gcaatgaaaa tattttaaaa atcaaaggaa
     6961 ataaagtaag tgcagttgga gttggtaaag ctacaatata tgctaagtgt ggaaataaag
     7021 aaattgagag aacatttagg attcaagcta gagttgctga aataaatatg gatgatataa
     7081 aaatagaagt tgaccagaat gaaaaattga aaccagatat aataacttct ccacatggtt
     7141 tagaaccacc aaaaatatat tatgagttag tagattcaaa attacctgta gaaagagcaa
     7201 taagtttaag tagtaatgga aatgttgtag ggcttagaga aggtaaagat agggtgagaa
     7261 taacttgtgg agataaatct aaagtaataa cagtaacagt agtcaaagaa actataacga
     7321 atgactatat acaaaacttg gttggttcac ataaaatagt aaacaataaa gttattatta
     7381 aacttgaatg ggattatatg aaagatgtct ttgattatga tatatatctt aaagataagt
     7441 tgtcaggaga atcatttaag aaaattaaat ctataactat aaatgaaagt gatttaggta
     7501 agtctaacaa ggtatatgca actatagaag tagatatgaa gggtaatcaa aatatagatt
     7561 ttgatattta tgtcataggt aaaaaggatt cagatacaac taagccaagt aatgtaataa
     7621 atattaccaa tggaaatgga aattcaaata atgataataa tattaaagat atgagggtag
     7681 aaaatatatc agctaatgtt gatagagata ataatgtcat tagagttaac tggagtagta
     7741 tatctcataa ggactgtact tacagtatat atgtaaaaaa taatactaat ggagatggag
     7801 aatttactct atatcaaagt gatatacagg gaaatgagca tactataagt attcctgaag
     7861 gagatttaaa tctagatatt tatgttgttg caaattataa tggaaatagc tctcaaaata
     7921 gtgatgttat aaatgtaaga tagatttgta tacaaattga aagtgttgag tttatttaaa
     7981 tattaaatta agaaaaagga tagtataggg taattatact atcctttttt gttacaatat
     8041 ataggtttaa aatctctagt aatataaatt ataaaaagtc ttatttacac aaatctatag
     8101 atattttttt tattaatcaa tattgtctta aattgaactt agtgcggctt ctattgcatg
     8161 tattactgtc gtatcaaata gtggaataga agtgtcattt tgtttaatta aaagacctat
     8221 ttctgtacat cctaatataa cagcctctgc accttgtttg ccaagcttat ctataataga
     8281 taagaaagct ttttttgaag attcagatat aattcctaag caaagttcat taaatattgt
     8341 gtcatttact attatacggt cttcttcatt tggaattaag acttcaattc cattattaat
     8401 aattacattt ttataaaaat cttgttccat tgtatattta gtacctaata aggccacttt
     8461 ttttatttct ttttccctta ataccgtagc agttacatct gctatgtgaa gtaatggaat
     8521 atgtatactt tcttgtattt tatctgatac cttatgcata gtattagtac agataacaat
     8581 aaaatctgcc cctgcctctt gtaatttaat tgcagcatca gctaaaattt tggcgctttt
     8641 ttcccaattt ccactagatt ggcattcttc aatttcttga aaatctacac tgtaaagtat
     8701 acatttagaa gagtgtagtc cacctaatct ttcttttatt actgtattaa taacttgata
     8761 atatgtaatg gtactttccc aactcatacc accaattaag cctatagtct tcatattata
     8821 cctctcttat ttatttattt tgaaatagct atttacaaat acaagtatag catctaaaat
     8881 agaagcatac aataataaaa ttgtatggag tacaattata attaagagga gtaattatat
     8941 gccaataaat tcttttgaaa actatcctat gacatggaaa ccaaagttag ataaccgaaa
     9001 accaccaata tataaaactt tagcaatgtt attagaagaa gatattaaaa gaggaaattt
     9061 aaatcctgga gataaattgc ctccacaacg ggaattagca gattttctag atttaaactt
     9121 aagtacaata acacgagcat ttaaactttg cgaagaaaag ggactaatat gtgcaaaagt
     9181 gggaaaggga acattcattt cttctgatgt aaatgtaagt aatacattat tatatcaaac
     9241 ggaatcaaaa gacataattg agttggggac agttcatcct ccgtatgagc aaaatacata
     9301 tattattgac tttattaaaa atgtactaaa acaaccagaa atggataggt ttttacaata
     9361 catgtctcca tctggcacat atatgcaaaa aaagtcaatt gcaaaatgga tagaaagaaa
     9421 taatgtatac accagtgaag aaaatatatt attatctaca ggagggcaaa atgctatatg
     9481 tgcaacttta ctaggattat ttaaagctgg tgatagaatt gctacagatt ctttaagttt
     9541 ttctggaatt aagtcaatag ctaaaatgat aggtattcaa ttagtcccaa ttcctcaaga
     9601 aaataatgaa atatcgattg aatatcttga aagttattgt aaaaatgaaa atataaaagg
     9661 catatatctt ataccagatt atcataatcc aacgacacat actatgtctg atttatctcg
     9721 taaaaagata gccaagatag caaagcaata taatataata attattgaag atgctataaa
     9781 tagcattttt agagatggta tacaaactcc catattttct ttagctagtg ataatactat
     9841 atatattttt agtacatcta aatttttatg tgctggtctt agagtagcat ttgtagttgc
     9901 tccaaaacga tatatagaaa atttagaaaa tgcactatat aatatgaatc taatggtttc
     9961 tccatttaca gcagaaatag tacaccgttt attatattct cctattatag ataaaattat
    10021 agaagagaaa agagatgcaa ttatagaaag aaatgaagtt gctgataaag ttttgtcaga
    10081 ttataatttg attggtgata agaattgcag ttttcgttgg ttacttcttc ctgatgaatt
    10141 ggatggaaaa tcatttgaaa tttctgcaaa aaatctagga gttcaagtat attgtgccga
    10201 acgattttca gtaggaaact ctacagttcc aaaagcagtt cgtatttgtg taacagctcc
    10261 aaaagatgta gaagaattag aaaaagggct taatataata aagtcacttc ttaagagtta
    10321 aatttatttt actattattt aattaaataa ataaaaaggc ggagaaattt attataataa
    10381 acctccgctt ttattattta atgaaatact ttacttttta ttaaaaaatt taattttaat
    10441 aatcttgaaa atataatcgt tttatggtaa aataattctt aaaaatagaa aaaattaatc
    10501 atttgttaaa aaaaatgcat aatttattgt aatttattga cgaaggttga aaaatattca
    10561 taaatgtgcg aaataacaga aaaaatgcaa aaattaattc aatttcattt gttataaaat
    10621 ctatatggca aaattaaatc tgcctatcag aatgatataa acacatcaga aaggaggacg
    10681 ctatcaaata atacaagtag ttaactttga tagcaaaaaa ttatgaatga acaaactaga
    10741 atatccttag agagagctgc tgaattaaaa tcaaaaattg atgattatat tcaggctaga
    10801 aaaacgatta acagaggtct tgaaaaagaa gaagagataa ataaacgaaa acagaaaata
    10861 ttaagtatct taaatggaac tgaagaggat tggaataact acaaatggca attatcaaat
    10921 agaataacag atgtagatac tttatcaaaa attataactc taactaaaaa agaaaaagaa
    10981 tatataaaag aggttggtac tcaatttaga tgggcaatat ctccatatta tttgagtctt
    11041 atagacccag aagatatatg tgacccaata aaattactgt ctataccaac acatatagag
    11101 ttggaagatg agcaagaaga tttggaccca atgggagaag agtatacaaa cccagcagga
    11161 tgtataacta gaagataccc ggatagatta ataataaatg taacaaatga gtgtgctatg
    11221 tattgtagac actgtcagag aagaagaaat attggacaac aagattctca taagtcaaaa
    11281 gctattatcc aagaatctat agactatatc agagaaaatg aagaaataag agatgtacta
    11341 gtaactggtg gagatgctct tacattaaaa gatgattatt tagagtggat tcttagccaa
    11401 cttaaagaga taccacatgt tgattatgtt agattaggta ctaggactct tgttacaatg
    11461 ccacaaagaa ttacagatga attttgcaat atgctaaaaa aatatcaccc tgtatatata
    11521 aatactcatt ttaatcatcc aatggaaata actaaggaat ctaaagaagc ttgtgaaaag
    11581 ttagcaaatg caggcgttcc attaggaaat caggcagtat tattaaatgg aataaataat
    11641 gataaatttg taatgagatg tttaaatcaa gaattactga aaataagagt aaaaccttat
    11701 tatatattcc aaagtaaaca tgtaaaggga acaaaacatt tcaatacatc agtagatgat
    11761 ggtcttgaaa tcatggagta tttaagagga tatacatcag gaatggctat accaacatat
    11821 atagtaaatg ctccaaaagg aggaggaaag actcctttgc ttcctcaata ccttgtatca
    11881 aaaggaacag attacgttat gcttagaaca tgggaaggaa aagttataaa aatggaagat
    11941 gaacctgctg tagatataaa gaaacttata aaagaacaag cacaggatta aaaaaatatt
    12001 taaagtttat aaaaagaaac aactttaaaa taaaaagaaa atggatgtca aatttatatc
    12061 aatttgcatc cattttttat tagttattta ttattttgtt gttgattaac taattcaaga
    12121 tatccacttg ggtggaagta atatatctca gaagtatttt catttacaaa gtaaccaata
    12181 tcaccatctg catctggtaa aaatacataa ccattaagat ttttagcttt taatgcttca
    12241 aaatcactag catttccttg atatatgtaa tcaacttcat tgttgtattt tactaaaagt
    12301 tctttagctt gttcttgagt caatttagta ttttggctta caggaacatt cttttgagaa
    12361 tctgcataag aagattcatt tactggtgca gttaataaca aacaagataa aactaataaa
    12421 gatataagtg cttttttcat actaaaaccc tctttcatgt ttaattatat aagattcatt
    12481 acttaataat attgtatgtt tcctataatt aaatattacc attaaagaga ataatattac
    12541 agttacacag ctgtaacttt tgattacaaa atcgtaatta aattgtattt ttattccatt
    12601 tgtattttat catgtctacg atttacaaac tcacagaagt atatctttat aacgcccatc
    12661 acaggagcag atagaatcat accaggtata ccaaagaatc ctccaccaac agtaactgct
    12721 aggattgtaa agaatgggct aagacctagt tgtccaccta cgatttttgg ctctataaat
    12781 gtaacttcaa cttgttgaat tattattaag taaattaaag aagttaaagc aattgttgga
    12841 ttggagaata aattaattat tacaactggt gccataccta ttactggacc aaaatatgga
    12901 accatattca tgacacccat aagtgtacca aataatagtg catattcaga ttttaaaaag
    12961 taaagaccaa ttccagaaag tactccaact ataaaagaat ctaaaatctt acctgtaaag
    13021 tatttaccaa tatttaaatt taagctttga catagttcta ttataaagtc tccatatttt
    13081 ttacctaagg aaatataaac tacttttttt gtaaacagta agaatttttc tttttctaat
    13141 aatatataga aacaaattat aaaacctaaa agaaattgta ctataaattt tccaactgaa
    13201 aatgtagtgc taaatatccc accaagtgaa ccaataagca agttactaaa tttaggcatt
    13261 acactcataa ttttgtcacc atactctttt aatgtattag ggtcaactga ttgtaaactc
    13321 ttgcctaagt cgaataaaaa ctgctctgtt ttatctatgt atgtaggaat ttgagcaact
    13381 aaatcagcta aactatttac tatggatgga actgtaaata atataaacga aattagtata
    13441 actatgagta caccataagt aagaagtaac gaatatattc tcttaaaatt taatttactt
    13501 tctaagaact ttactagtgg gttgaatata tatgctaata caaatgctat tataaatggt
    13561 gttagtagtg acattaataa acttatcact ccaaaaaaat atttgtaatt atcaataact
    13621 ttaatcaaga tatatgaaac aactacagtg agtacaatat ttagataaat ttttttatct
    13681 tgcaaaataa cacaaccttt cttatattta ttaaatttag atatacttaa atctaagtta
    13741 aagttaatat ttatag
//
LOCUS       DAEMGG010000104        13521 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEMGG010000104 DAEMGG010000000
VERSION     DAEMGG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.105"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            329..1030
                     /locus_tag="KPK24_002828"
     CDS             329..1030
                     /locus_tag="KPK24_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG2300481.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1300..2118
                     /gene="larE"
                     /locus_tag="KPK24_002829"
     CDS             1300..2118
                     /gene="larE"
                     /locus_tag="KPK24_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG2300482.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2173..3321
                     /locus_tag="KPK24_002830"
     CDS             2173..3321
                     /locus_tag="KPK24_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG2300483.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3457..4845
                     /locus_tag="KPK24_002831"
     CDS             3457..4845
                     /locus_tag="KPK24_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2300484.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4874..5572
                     /locus_tag="KPK24_002832"
     CDS             4874..5572
                     /locus_tag="KPK24_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300485.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5575..7008
                     /locus_tag="KPK24_002833"
     CDS             5575..7008
                     /locus_tag="KPK24_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2300486.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            7030..7767
                     /gene="truA"
                     /locus_tag="KPK24_002834"
     CDS             7030..7767
                     /gene="truA"
                     /locus_tag="KPK24_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG2300487.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7878..8085
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8338..9537
                     /locus_tag="KPK24_002835"
     CDS             8338..9537
                     /locus_tag="KPK24_002835"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG2300488.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9784..10830
                     /locus_tag="KPK24_002836"
     CDS             9784..10830
                     /locus_tag="KPK24_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG2300489.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10938..12101
                     /locus_tag="KPK24_002837"
     CDS             10938..12101
                     /locus_tag="KPK24_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2300490.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12190..12570)
                     /locus_tag="KPK24_002838"
     CDS             complement(12190..12570)
                     /locus_tag="KPK24_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG2300491.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12573..13232)
                     /locus_tag="KPK24_002839"
     CDS             complement(12573..13232)
                     /locus_tag="KPK24_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG2300492.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         5434 a         1341 c         2304 g         4442 t
ORIGIN      
        1 catatcaaca aacatattac ttcttattac aaattatatc atatgtttta tattaaaata
       61 ttataatatt acaacgaatt gttacaaaat tacttattat taaccatgaa catttgtttt
      121 gtgctatcat atttatataa gggaattgtc ataaaaaata tcttgttcat acagttttat
      181 atagaaaaaa gaaatgaatc ttttctttat ataagtatat tttgtaaatt gcctctatat
      241 ttatagttta aataatagtg gtattaaaaa tctgacaaca aaaataaaac tttcaaaaaa
      301 ttaaatctaa aaagaatagg agataaatat gagtattgaa gatttatatg attttgaatg
      361 tgttcctttg aacaatgaga ttgatattga tgttttattt gaaaagatga tagaagatgg
      421 aaaagaaaaa gaatatacac caattatagt cattgatgaa aaagtagggc tattaagaaa
      481 aaatatagat atgattacta aagagtatgg ttctcttgaa aactatagag agtattgttt
      541 aacaaaatat aatgaaattg atgtaaatca attttttgat tttaggaaga gtgagatttc
      601 agaattatta aaggagctaa ttgatttaga agatgatttt tatgattata aacattttag
      661 acccattatg aatatagata tatttcatga atataacaaa gtttacatag ctaaaattcc
      721 aacaattaaa ccatatgaag tatttgcata catacctatt ggcggattta atgattgtcc
      781 tagagatgaa gaacatatag ctattgcaaa atattggtat gaaaagcatg gaatctttcc
      841 aatcgcaatt ggatgtgata cagttcaata ttctgtaaaa aatttaacta aagatggtaa
      901 aaaatttgat gacttatgta ttgaattagt attttattgt gaggatatta ttatacaagg
      961 gtatgaaacc ttaaaggctc ttaaggatgt attaaaacgc tctacaatag tgcttttttg
     1021 gtgggattga tattctatca tttagatagt attaaattgt tagtcaccaa gaatccactt
     1081 gctttagtta tagggagtgt gtcaaactag gtttttatac acagtgtagc atagtaatat
     1141 gtttaaatta caaaggttaa aaaaatactt taatattttt aatattataa ttaatagatt
     1201 taaggtaatt ataatttata aaataaatac tttatatgtt ataatcatta agaatgtaaa
     1261 aaaataatat atagttttat agttcatatt tttccagata tggaggtaaa ttttgtgaag
     1321 gaaaaagaaa aattagataa attaaaaaaa atgttacttg aattaggcag tgttgtagtt
     1381 gcttattctg gtggagtaga tagtaatttt ttgcttaagg tcgcaaaaga tacattaggt
     1441 gaaaatgtta tagctgtgac tattcatgca atgatgcatt caagtaggga aattgaagag
     1501 gctaaacaat atactcaaaa ttttggtgtt aaacatatca tattaaatat agaaaacttt
     1561 gatttaaaag aatttaaaga aaatggtgtt gatagatgtt atcattgtaa aaaatatata
     1621 ttctctaaaa taaaagaggt agcaaaagaa cataatataa agtacatagt agatggaaca
     1681 aatatagatg atttaggaga ctatagacct gggcttaagg cattgagtga attaggagta
     1741 attagtccat taaaagatag tagtttaaaa aaagaagaaa ttaggtcgct ttcaaaaaca
     1801 ttaggattaa atacttttaa taaaccatca tttgcatgtc ttgcgagtag gataccttat
     1861 ggagttgaaa taacagatga aaatttaaga atagtagaaa agagtgaaga atatctatcc
     1921 aatttaggtt tttctcaatt tagagtgaga atgcatggag atatagctag aatagaagtt
     1981 ggacaggaag agctaggtaa attttttgaa aataacaatt ttaataaagt tgatactaaa
     2041 ttaaaaatat ttggctttaa atatgttaca ctagatatgt caggatataa aatgggaagc
     2101 atgaatttaa atgtgtagat tattttaaaa ataaataagt tttatagtta atataaggaa
     2161 acaggaggtt ttatgaaaaa ttatacgtta atatcgccat gtttttttgg aatggaaaaa
     2221 atgctggcta gagagattac taatttagga tatgaaataa taaaaacaga agatggaaga
     2281 ataacatata aaactgatga gtttggaata gcaaagtcaa acatgtggtt gagatgtgct
     2341 gaaagagtac atcttaaaat tgctgaattt gaggcaaaaa gctttgatga attatttgaa
     2401 aatacaaaaa gaataaactg gtctagatat ataccttatg gagctcaatt tcctatatca
     2461 aaagcttcct ctataaaatc aaaattatac agtacaccag atgtacaagc catagtaaaa
     2521 aaggctatag tagaaagctt aaagaaaagt tatttggagg atggtttgct taaagaagat
     2581 aaggagaaat accctatttt tgtatttata cacaaagata aagtaaccat ttctatagat
     2641 acaacaggag atgctttgca taagagagga tatagagaaa aggcaaataa agctccaata
     2701 agagagactt tagctgctgg gcttatttat ttaactccct ggaaagcagg tagagtttta
     2761 gtagacccta tgtgtggttc tggtactata ttgattgaag ctgctatgat tggtataaat
     2821 atggcacctg gcttaaatag agagtttata tcagaaaagt ggagaactct tgataagaaa
     2881 atatggtggg atgtaagaaa agatgctttt aataagatag ataatgaatc taaatttaag
     2941 atttatggat atgatataga tgaagaatct atagatatag ctagagaaaa tgctgaaata
     3001 gcaggtgtag atgaatatat agagtttaat gtaggagatg caactcaatt taaaagtgaa
     3061 gatgagtttg gatttataat cactaatcct ccttacggag aaagattaga agataaagac
     3121 agtgttaaac agttgtataa ggagcttggt tatgcattta gaaaattaaa aaattggtcg
     3181 tattatttga taacttctta tgaagacttt gaatatgagt ttgggcaaaa agcagataaa
     3241 aaaagaaaat tgtataatgg tatgttaaaa actaactttt tccaatatcc gggtcctaag
     3301 cctcctagaa ataataaata agttttacat gatttatttg taaaatataa aattttatta
     3361 aaataagtaa aacattatga tgccctgaaa cgtctaatta ttgtgaggag atttttgtga
     3421 acaaatttaa agatttatag gattaggagg agttaaatga aagtgttaaa gcttattact
     3481 gtagtagccc tgatgatgtt atttgttact ggatgtaata taaaaactga atctccagaa
     3541 caattagcga agatacctga ctatgatgat acaaaaaaag tattgtatga tggtattgac
     3601 cagttactaa aaccagattc atctatgata ttgccttcta atacaaaaga agtaggaaaa
     3661 ataaataagg tagatttaaa cagcgatgga attgatgaat tagtagtatt tgaacaaaaa
     3721 gaagatttaa gtaataatgt gagtcaggta gggtttgtaa ccttgagtta caatggagaa
     3781 aaatacgtac ttggagacca ttttttagaa aatggcgaat caatagaata tgctaatttt
     3841 tatgatttgg actcagatgg atataaggaa gttatcttat tggttaaatc caaagacaaa
     3901 acgaatatgc atatatataa agtagtagat aatgaaataa caaaaatata tgacttggat
     3961 gcatcatggc ttcaaaataa agaagatttt aatgacatga aagtaaaaat tggatatata
     4021 gatggagatg acaagttaga catattgatg cttcatttga ataataagac taatgaaatg
     4081 tttgcaagtg ttgcaaattt tgatgggaaa atgaaaatca aagattctgt taagtttgaa
     4141 aatgtaaaaa acttaagtga attatatata acattaggta atgttgcttc atctgttgga
     4201 aattcttctg cagctataaa aggtattgta cttgatattc ctatattgaa agataataac
     4261 tatattacgc agatacttta tatgaaagat gggaaaatta acaaagcttt tagtgattat
     4321 gataaaacta ttactaaatc ttattatata cctgtagatg atattgacaa agataagatt
     4381 atagaaatac ctatagttgc aggcagtaca ggtaataata aaaatactta tagttctaaa
     4441 tcatctgcaa ctataagttg gtatagatgg aatggaaaag aaggtgctga ctcaagttta
     4501 atatttacaa gtcagatata ttataattat aaatataatt ttaaattatt tattccaaat
     4561 aatttatttg ataaaatact tgttgaacag gaatttgttg gagaaaaagc agtatttaaa
     4621 ttttactatt ttgactattc agaccgtaag aatttattta atatagtagt agagagtaaa
     4681 aataaacttg aagatagaaa aaatacaggt actcaaaatt ctattgtact tcaagagagc
     4741 gatgaatata ctttcttact agttgtaaac aatgcaaatg aaatggataa gctagatatg
     4801 actatagacg cattaaaaga atatttctca ttaatatatg gttaatatta tactcaattg
     4861 gaggttttat tttatgaagg aaaagatact tatactagaa gatgaaattg gtattagaag
     4921 ttttgttagt ataaacttaa aaagggaagg ctatgaaata gttgaggcag gtactggaag
     4981 agaagctata gaaaaaatga caacagaaaa ggatattaca atagctcttt tagatgtaat
     5041 gcttccagat ataagtggta tagaagtatg taaatttata agggagaatt ttgaccaagt
     5101 aggtataata atgcttactg ctaaagctca agaagatgat aaaatagaag gatttatctc
     5161 aggagcagat gattacatca taaaaccatt tagtataaaa gagcttttag tcagagtttc
     5221 tgctcttctt agaagagtag caaaggatga ttcaagtgtt aagtcgagtg agatagtttc
     5281 tccaccattt atacttgata tagacaaaag aaaattattt aaaaatggaa aggaaattga
     5341 gcttacacca actgaatttt caatagtgaa atatctaata tcaaatgcta agcagtcgtt
     5401 aagtagagac cagatattag acgaagtatg gggaactaac tatttatatg attttaaaat
     5461 agtagatgta aatataagaa ggataagaaa taaaatagaa gatgacccat caaaaccaaa
     5521 atatatacaa actatttggg gttatggata ctgttttaga aaggaagaat aatagtgtta
     5581 cattttgaag gtctgagaaa aaaagtaata aaaaactatt ttataataat tattataatg
     5641 gttactctgt ttgaaggatt gttcatgttt tacatacaaa actactatta tgattctgtc
     5701 aaacagctac ttgaatctga aataaaatat gctgatgaat ataatgctat tacaatggaa
     5761 actacaagtt ttgagaaaaa agtaaaaaat atatttgata aacaaccctt aactaaaaat
     5821 tcagaatttg gaatttcaat aatagataaa gataaaaata ttatattaga ccaatatggt
     5881 tttaaaagta aagaaaaagc aaactatgaa gatgttaaca atgcattaaa agatataaaa
     5941 actaaaaatt taacacctta tacttataga ataccagata caggggagca tgttatgagt
     6001 atctccttac cacttaaggt aaataatata atagaaggtg ttgttagata tacagtatca
     6061 ttagatgcta ttgataatgc aatattaaag caagctacat ggcttatatt agcaggaata
     6121 tttatactta ttatagctat actaataagt cttaaatttg ctgagactct tataaaacca
     6181 ttacgagagc taaagaaatt tgcaaatgaa ttggctgtag gaaattataa tataaaatta
     6241 gaaaagatga agattgtaga tgatgaaatt ggtgatttag cacagacatt tgaacacatg
     6301 gcacatgaga ttgacaaaag tgaaaaatta aaagaggaat ttatatcatc tgtatctcat
     6361 gaactaagga ctcctcttac atctataaag gggtggagtg aaaccttagg ttatgaaagt
     6421 ataacaagag aagaactaga cttaggttta ggcattatac aagatgaaac agaaagactt
     6481 ataaaactag ttgaagaatt attagacttt tcaaggcttt cttcagacag gataaaactt
     6541 catgttgata tagttgatgt tgaaggttta attgtaggtg ttgtaaatca acttaaagtt
     6601 aaagcagctg aaaaagatat atcactttta tttgaatttg aaaatgaatt tatagagaac
     6661 attcaaggag ataaaaatag attaagacag gttctcataa atttaataca aaattcattt
     6721 aaatttacga gtcaaggtgg ttacataaaa gttgtagctt ctcaagatga agagataaca
     6781 accatatcag tagaggataa tggttcaggt atagaaaagc aaaatttaaa taaagtgtta
     6841 gataaatttt tccaagaaga ttataataag gctggtagtg gattaggact agcaattagt
     6901 aatgaaattg taaaacttca tggtggaaga atgaaaatag aaagtgaaaa gaatgttgga
     6961 acaaaaataa cttttaatat aaaaaataaa tttgctaaac aagcataaat tcttagaaag
     7021 aggattaaga tgagaaacat aaaaatgata gtagcatacg atggttcaag atataaagga
     7081 tatcaaaaat taggtgacaa taatatgact attcaagaaa aacttgaaaa tgttttaagt
     7141 aaaatgacta atgaaacagt ggagataata ggttctggaa gaactgatat gggagctcat
     7201 gcaagaggac aagtggtaaa ttttagaact aattgtatgg attctttgga taaaattcaa
     7261 aaatatttat atgaatattt accagaagat atagttgtaa aaactgttga agaagttgat
     7321 gaaagatttc attcaagata caatgtaaaa tcaaagactt atatgtataa aattgacaat
     7381 aataagtatc ataatccttt tataagaaaa tatgctactc atgtaagtaa aaaattagat
     7441 ttagatagaa tgagaaaagc aagtgagtat ttagtaggag aacatgattt tactagtttt
     7501 gcttcttcta aatctaagaa aaaatcaaat gtaagagaaa tttattctat aaatataaaa
     7561 gaagatgata atgttataga aatatatgta gaaggaaatg gatttttgta taacatggta
     7621 aggataatag taggtgcttt gatagatgtt ggtctaaaaa gaagagctcc acaagatatt
     7681 aagtatatgc ttgaatcaaa agatagatgt caatcatcag atactgcacc agctaaagga
     7741 ctatgtcttt ggaaagtaac atattaatca attatataaa actctcttta aaataaaaat
     7801 aaaataaatt atatagttga cataaatgga aaaataaact attatatatt ttataagaga
     7861 gatataaaac tttatattta aatcctgtga gaaagagagt aacttctatg tgttttatag
     7921 agagctgagg atggtggaag cttggcaaat aagtattagt gaaaagaact ttggaggata
     7981 ctaattgaat ttagtatgtt agtaggtaat ctcgccttaa gagaaagagt ggataagttt
     8041 atcttatcaa ttagagtggt aacgcggata tatttcgtct cttggttttt ccaagaggcg
     8101 ttttttaata tatttaatta ttagtgttta tctggtatgt tgaacattta ctaattgatt
     8161 agctaagttt atcaataaga gtggtaccgc ggatatttcg cctcttagta ttttaaaata
     8221 ctaaggggtt tttttagtat taaaatgatt ttatgtgagt ctaaattctc agagtcattc
     8281 aatattaaga atttctataa aataaaaatt ataatttaag attgggagga attaaaaatg
     8341 aaggaaaaag tagtattagc atattcagga ggacttgaca catcaatcat aataccttgg
     8401 ttaaaggaaa attatgaaga tatagatgtt atagctgttt gtggaaatgt aggtcaggaa
     8461 gataaaatgg aagatgtata tgaaaaagct ttacaaagtg gagcatcaaa ggcatatgtg
     8521 gatgatataa gtgaagaatt tgtaactgaa actatattta aagctgtaaa agcagaggct
     8581 aaatatgaag gaaaatactt attaggaact tctttagcaa gacctataat agctaaaaaa
     8641 ttagttgaag ttgctcataa agaaggtgca aaatatatat gccatggatg tacaggaaaa
     8701 ggtaatgacc aagttagatt tgaagctaca attgcagctc ttgacccaac tataaaagtt
     8761 attgcacctt ggagaatttg ggatataaaa tcaagagaag atgctataga ctatgctgaa
     8821 aaacataata taaaggtaac agcaactaaa gctaaaatat attcagtaga tgccaacctt
     8881 tggcatgttt caacagaagg tggagatata gagcacctag aaaatgaaca taaaaaagat
     8941 gtttataaac aatgtgtaga cccagaagat gcatgtgatg tagctgaata tgtagaagtt
     9001 tactttgaaa aaggtgtacc taaaaaggta aatggcgaag aattatctcc agttgcactg
     9061 attcataagt taaatgagtt gggttgtaaa catggaattg gtgttataga tatagttgag
     9121 aatagacttg ttggtatgaa atcaagaggt atatatgaga ctccaggagg aacaatcctt
     9181 tatgaagcac ataatatttt agaaagtgct actttagaca aagatacatt acatttcaaa
     9241 caaatggtat cttataaata cggagaactt atttacaatg gactatggta ttgtaaatta
     9301 agagaatcta tagatgcatt tatggaacaa actcaagata atgttacagg aacagtaaaa
     9361 gttaaacttt acaaaggaaa tataaaacct gctggaatat ttactgaaaa tgctctttat
     9421 gatgaaggaa tatcttcttt tggaaatagt gaactttatg accacaaaga tgcagaagga
     9481 tttattaatt tattcacttt accattaaaa atcagagcta tgaaagctgg aaaataataa
     9541 aataaattat ttaagagtgt ataaaaacga tttttaggtt tttatatgct ctttttttat
     9601 ttgaatttat taaattatac agtaaatagt ataaaaaaat atattatagt tccaaatggc
     9661 ttaataatgt gaaaatataa gacctaaaat ataaatttta taaatacaat agttattgac
     9721 tctctcattg taagagggtt tatactgaat ctaatgaaaa tttatagtga ggagatagat
     9781 tatatgagaa aaataattga aattaaacat cattcacatg attatgagtg tatgtggaat
     9841 ggtatagaag atttatatat aaataaaacc ggtgaatacc ttccaaacga ttttttcttt
     9901 ttactgtcag gttttggttc attttgttat atgaaaacaa ataaagctga tttgaaaaga
     9961 atggtagctt tgggagatgg aaggacaaaa cagatgtata aatttttagc accaattgtt
    10021 ggatttgaat acaaacatca tgagtttaaa aagtatgagc aagcattaaa gaaagcaaaa
    10081 tctgaaattg atgatggata tccagttatt ttaggagctt tagatatgta ttatctacca
    10141 tattataaga agctatatca taaagaacat attccttttc attatatatt aatggtagga
    10201 tatgatgatg aaggaatata tctgtatgat tgtggtagaa cagaattatt acatctttct
    10261 tatgataaat tacgtgaaag catgaattgc aattcccctg gattaagcaa agaaaatact
    10321 atttgcacaa taagaatgaa tacttcaaag aataaatata acattgctag agaagctatt
    10381 gctataaagc gtgatatgtt tttaaatcca cctaaaggat ttttgggata taaaggtttt
    10441 gaaaagttta ttaaagagtt gccacagtgg aacactgaat tggataagga tgattatgat
    10501 aagattttga ccaatatggt tatgtttttt ggaacagtac caacaattcc aaatgcaatc
    10561 aaaggaatta aagaaccaga tactgttcaa tttaaaggtg gatttgataa aatgagtaga
    10621 atgctcaaca gtattgggga ggaatacaaa aacaactctt ttatacaagt agctgatatt
    10681 tttgataaaa ctgctattat aattgagaaa atatccaata ttataataga ttacttaact
    10741 caaacatgtg atgatacaga acaacttccg ttattgttct ctgaggtctt agagcatatg
    10801 aaaacaggat atttaatctt ggatgtataa aaaatatatt gttgacccta acattacgta
    10861 atagtgtaat atgaaaatat agcttaaata tattagtagg tattgatggc tgtaaaatta
    10921 aaatttgagg tgtggatatg agaacagtga aacaagtttc tgatttaact ggaataagtg
    10981 tgagggcatt acattattat gatgaaatag gtcttttaaa accaaataaa attacagatg
    11041 caggatatag actttatgat gatgaatcta ttaaaactct tcaacaaatt ttatttttta
    11101 aggagattga tattccttta agagaggtta aggaaataat gtcaagccaa tattttgata
    11161 aggttgaagc tctaaaaaat caaaaaaaat tacttatttt aaaacgaaag agactggatg
    11221 aattgataga gcttataaat caaactctaa gaggagaagg aaatattgat tttaaagagt
    11281 ttgatatgag cgagtatttt aatgttttag aagaatttaa aagagagcat aggaataagg
    11341 taattaaaat ttatggtagt gtagaaaaat acaatgaata tatagaaaga gttaagtcta
    11401 atgaagagaa aattgctaaa atggctataa aacaatatgg aactattgag aagtttgcta
    11461 aagctataaa aacaaatttt agcagtgata tattgaattt aggagaaaaa tttgatagat
    11521 ataaaaatga ttgtttaaaa gaaaaacatc caaaattaaa agaattatat agaaagcttg
    11581 tagaagattt aagtaggaat ccttcttcaa cagaccttca agaaatagca aaagcgataa
    11641 cagatatatc taaaaaagat tatgaaattt tctctatgga tactggtgat gacaactggt
    11701 attatatggt tcaaaattat ctagtaaatc ctatgtggat agaagaagta gataaaaagt
    11761 atggaagtgg agcaggaaag tttattggac aagtactaaa gacttatcta agagatagaa
    11821 aaccaaagat aaatacattg tatgaaaaac ttgtagaaga tttgagtaga gattgttctt
    11881 caagggaagt tcaaagcatt gttgaagaaa tagacaatga aatgaaaaga agtaatgaat
    11941 tttataaaat agacaatggt gaaagatact ttgattatat gtcagagctt tatttacaag
    12001 attctaacta tataaaagta actgataaga attatggtga tggtgcatct aagtttattg
    12061 gagaagcttt taagatttat tttgataata ataattgttg aaaatttgga agtaaaaaat
    12121 atgtaagttt tataaaagca caaaaataag gatgtagatt agctccatct atatccttat
    12181 ttaaagttat taaaatatat agtttacatc aaacttaaca ctttcaataa tatttcccaa
    12241 atatgttttt atactttcaa cttcttctaa agattttaat ttattttcag tataaattgc
    12301 tttgtccaga atattaaacc ttaatcctat ttcatcaaag tactcatgat ttaaaacttt
    12361 gtaccaatgg tttttttctt tattccaact cgtagatatt gaatcagctt ctttttgagc
    12421 tttttcccaa tcttcatttt ctatagattt ttctaaaaca gatatattgt ctttatatcc
    12481 ttctgtaaaa tcatatagtt tattgttaac gtaaaatcca aataatacaa ataagatagt
    12541 ccatagtata acaaatgttg tagatttcaa tattaatcac cctttttatt ttgaataaat
    12601 attttgtcat tttcatctaa aacacaaata agaacatctt taaaagtttc tatgtgatta
    12661 gattttaaaa tacccattaa ccagttagta tctttttgag ctatgtctag atttttattt
    12721 attattctac catccagtat aagtgggata ggtaaatgat taaaagctct tggaggagta
    12781 ctattgtagc ttgatgctgg tacaacactt agattaccat cagtctccaa aatagcatat
    12841 tgtacatctt ttaggttaaa gtatccttga atcctaagtt gttctaataa ttcatctata
    12901 ctaattcttt cttttttaag ctctttatat acaatttttc ctttatctat taatacagaa
    12961 ggtttacctg ataaaaatga acttaatttt ctgcttttta atgataagaa tgaaattaga
    13021 gtctgcatga ttacaagtcc agatattgct gcaacaccat ttatgattgg tatattatta
    13081 ttttccattg ggacagatgc aacttctgca attaatagtg ttataactaa atcaaaacag
    13141 ttcatttcag ctatttcacc ttttcccatg actctaagtg caattagcac tgttatgtat
    13201 aatataatac ttcttattaa tactactgtc atatctatca attactcctt taaaaaatat
    13261 ttacacttag ttagtatatc ctatataaaa aatttaattc aaagtataaa aaaccttaaa
    13321 taattggatt caactattta aggttttttt gataatttta atattaaatt ttttatggat
    13381 tttctgttac ttttatttat tttaataaca gattcaacaa aactcttttt atctcctaat
    13441 tttaagataa aatcaggata ttcttcaaat tcattgagtt ttattaattt gaaagaagaa
    13501 gatagatttt taaagttaat a
//
LOCUS       DAEMGG010000105        13488 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEMGG010000105 DAEMGG010000000
VERSION     DAEMGG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.106"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            885..2243
                     /locus_tag="KPK24_002840"
     CDS             885..2243
                     /locus_tag="KPK24_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG2300493.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2350..3144
                     /locus_tag="KPK24_002841"
     CDS             2350..3144
                     /locus_tag="KPK24_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG2300494.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3137..5443
                     /locus_tag="KPK24_002842"
     CDS             3137..5443
                     /locus_tag="KPK24_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2300495.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5582..6859
                     /locus_tag="KPK24_002843"
     CDS             5582..6859
                     /locus_tag="KPK24_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2300496.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7042..7764
                     /gene="yqeC"
                     /locus_tag="KPK24_002844"
     CDS             7042..7764
                     /gene="yqeC"
                     /locus_tag="KPK24_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG2300497.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSLINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            7871..8440
                     /gene="mocA"
                     /locus_tag="KPK24_002845"
     CDS             7871..8440
                     /gene="mocA"
                     /locus_tag="KPK24_002845"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG2300498.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8437..9456
                     /locus_tag="KPK24_002846"
     CDS             8437..9456
                     /locus_tag="KPK24_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG2300499.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            9794..11419
                     /locus_tag="KPK24_002847"
     CDS             9794..11419
                     /locus_tag="KPK24_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2300500.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            11618..12094
                     /locus_tag="KPK24_002848"
     CDS             11618..12094
                     /locus_tag="KPK24_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300501.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(12302..13414)
                     /locus_tag="KPK24_002849"
     CDS             complement(12302..13414)
                     /locus_tag="KPK24_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2300502.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
BASE COUNT         5272 a         1429 c         2415 g         4372 t
ORIGIN      
        1 gcttattaag tttttctgtc ttatgagaat tatattataa aatattcata aatattttga
       61 gtttaaatta aataattatt ttatgcttag attaattgat atttaattaa tttaataaca
      121 aaaacagtga aagagtaaat aatattattt acagggggta aagcaaagta tgttaaataa
      181 attgtacttt tggccccgta catagagaaa tctatgtagg acacaacttt aaaaccagta
      241 aatcctaaag ccttgcaact acaataaaag atagaaagaa acttttatga atgtggtgaa
      301 aaccagaaac aatagtgagg atggtatatg gtgagagcct aagtacttaa aaatggacat
      361 ttggtcgcca agcctagaaa taggaaggtc aaacgactat ctcgagagag agtacactat
      421 aagcgattga tagtggaaac gggttgctct tagcaggtaa tgctgaagat gaagaaatag
      481 tctacgctca tatgaaagta tgaggtcact ggatttgaag tgacatctat aaagtagcgt
      541 ttataggtaa atgtttaaaa cttgaaacat gtaaatactt atgaaaactt ataacttata
      601 atgattaaga ttgaaagatt taaattgaat taaatttact ataaaaaatt aaaaattcat
      661 agctataata ctcttatatt tgtaaagcat ggtatataat gtatacaaaa ttaaagaata
      721 cgtttatttg ttaataatta aatttattgc atctgttttt taagactaaa aacttaaata
      781 tatgttggag tatagttgat atttatgaac atcattaatt taatatatta gatttttaag
      841 tatttatata attcaataag ttttatgtaa cgggagtttt tattatgaaa gcaaaaagta
      901 catcaaaatt tgaattagac ggaataccac cactaaagga agcaatacct ttagggttac
      961 aacacttaat ggcaatggta ataggtagtt gtttaccagc gatattgatt gcaaatgcag
     1021 ctggacttaa tcataagatg tcaacattat tgattcaagc cgcattagtt tttgcaggat
     1081 tatcaacttt attacaatta tatccaatac ctttgtttaa aggttttaaa ttaggaggag
     1141 gaataccagt tataatggga gctacagcta tgtttatagg agctggggta tctgttgcaa
     1201 gtaagtatgg actaccagta ttatttggct ctcaaataat agctggtata gtaataatta
     1261 taattggttt tggtcttaaa aaaatacgat ttatatttac tccagctgta tcaggaacaa
     1321 tagttgcatg tatgggtctt ggactttttc caatagctat aaaaaactta gctgggggta
     1381 tgggcaatga gacttttggg aatccaatta attttatagt tggaatatct gtatctctta
     1441 tgatattagg attaaataag tatggaaaag gattatttaa agatgcatca atattagtta
     1501 gcataatttt tggatacatt ttatcattaa ttttgggtat agtaaatttt tcttcaattc
     1561 aggagtttac tttggttgca cttccaaaac cacttgcttt tgggctagat ataagacttg
     1621 aagttgttgt aatgttttca ataatatatt tagttgagat agctgatata atgggtgctt
     1681 gtacactttc tgcggtagga gggcttaata gacaagtaac agatgaagaa ctttcttctg
     1741 ctgttttagg aaatggagta ctctctacaa taggtgcatt attttctgca actccaatgg
     1801 ggatgtttgg acaaaattct gcaatcgttt ccaatacaaa ggttgtaagt aaatttgtac
     1861 tagctattgg aggaattgga ttatttttgg caggaatatc gcctttatta gctaatttaa
     1921 taagaacaat tcctccctgt gtggttggag gagcaacact cgttatattt tcaactctta
     1981 caacatctgg tttaagactt gttagtatgg atggatttaa tcaagaaaat agcatgatat
     2041 taggtctttc tatggcttct ggtattggat tcatggtagc tcctcaagtt cttgaaaaat
     2101 ttccaaaatt tatagagaca ttacttgcag attcaagtgt agtttcagga gcaatggtag
     2161 ccataataat tcaagcttta tatatggtaa aatttcctgg aaataaaagt gataaggttg
     2221 aggataaaaa taaaagttta taatgtaaag gtttaatata tgtgaattaa atatactaca
     2281 attataaata taaataaaag atttattaaa aacttttgtg ttttaataag ttaatgatgg
     2341 ggggataaga tggttactat aaaggaatat tcagttccac agagtttaga agaagcatat
     2401 aaaattttaa tttctaaaaa aaataatgtt attttaggtg gatgtggatt tataaaatta
     2461 agtaataaaa atataggaac agcgatagat ttaaaagata taaatctaaa ttatataaaa
     2521 gaggataaaa aaaatatttt gataggtgca gatacttcac ttagaagttt agaaattgat
     2581 aaaactataa aaaattactg tagtggaata ctttcaagcg ctgtttctaa tatagttggt
     2641 gttcaattta gacaaggggc tagaattggt gcaagtgtat tttcaaagta tggcttttct
     2701 gacttaatac cagcgctttt ggttgttgga gcgaaggtga gattatacaa taaaggaata
     2761 ctagagttaa gtgagttttt ggatagtgaa ttaagtaggg atatattggt cgaagtaata
     2821 ctaccaaaaa aagatgcaat agctgttttt gactcaataa gaaaatgcac aggagatttt
     2881 tctgtattaa attcagcaat gctaaaagaa aatgatactt acaaaatagc aattggggca
     2941 agacctcaaa aagctagatt agcacttgaa gcgagtaata tattaaataa agaaaaagat
     3001 atagataagg catctattgt tgctagtaag gaacttactt atggaagtaa tatgagagct
     3061 agtaaagagt acagaaagga tatggcaagt gcattagtta aaaggatgta taatgctatt
     3121 gaggagggaa tgtataatga ttaataaggg gataagaaag atagatagta cagcaattgt
     3181 cacaggaaaa cctctatata ctgaagacct cattatgcat aaagatgttt tgacattaaa
     3241 actacttaga agtccacatg ctcatgctaa gataaagaat atagatacat caaaagccga
     3301 aaaaattcca ggggttgtgg gtatttatac gtataaagat gtaccacagg tcagatattg
     3361 tgtgccaggt caaacagaac cagaagcatc accttatgat agagttttac ttgaagatac
     3421 agttagattt attggagatg aagtagcttt agtggctgct gaagatgaga aaacggcact
     3481 taaagcctta aaaatgataa aggtagatta tgaggtttta aagcctgtgt ttgatgcaag
     3541 agaagcagaa ggaaatgaag ttgttataca tccagaaaaa gacatagtag agccatatcc
     3601 ttttggattg gatgcaaaaa ataatatagt atcaaaagaa ttttttgaat ttggagatgt
     3661 tgaagctaag cttaaagaaa gtgattttgt ggttgaatct tcatacaaaa cccaaagtca
     3721 atcgcattgc atgatggaaa ctcataggtc atattcatat aaagatataa atgatagaat
     3781 tgttgtagtt tcatcattac aatcaccaca caatgtaaag agaatactta gaaaagtatt
     3841 gggtatacca tcttcaaaaa taagagtaat taaacctaga ataggtggtg gatttggagg
     3901 aaagaatata gcagcaacag atatatttac tgcatttgtt actatgaaaa ctggtagacc
     3961 agcaaaattt gtatttacaa gaagtgaaac ttttgtaggt tctaatagta gacatgaaat
     4021 gttttttgat gttaaagttg gtgcagataa agaaggaaat ttaaaagcaa tagagctaaa
     4081 gtcattaaac aatacaggtg cttatggtgg gaatggtgtc tctgtttctt ccgaatctgg
     4141 acacaatacc ttaccaatat ataatgatat tgatgctgtt agatttaatg ctagaacagt
     4201 atatactaat aggttaccag caggagcact tagagggtat ggtgctacac agggaatatt
     4261 tgcaatagat agtgctattg atgaattggc acataagatt ggaatggatc cacttgaatt
     4321 tagaattaaa aatataatag ataggtatac caatggaaaa gcagctgaag acgaaatatt
     4381 aagttgtgag ctgagacaat gtatagaaaa gggcaagaaa ttaattaatt gggatgataa
     4441 atatccatgt aaagatatag gaaacaacaa agtaagagca gtaggtatgg cagtagcaaa
     4501 tcatggttct ggtataccgc gtgtagatat ggcaacagtc actataagaa tggatgaaga
     4561 tggcacatat aaactattat ctggtcttgc tgacttaggt caaggggctg atacaattca
     4621 agctcaaatt gctgcggaag ctttaaatac tactgtagat agaatttcta tttatacagg
     4681 tgattcagac ttatgtccct atgatacagg agcatttgct tcttgtacga caacggttac
     4741 aggaaatgca acaataaaga cagcaaataa attaaaagat atattacttt cagttgcaga
     4801 gtcaaagcta ggatttaaaa aagaagattt agagttggga aaagacaggg tttttgttaa
     4861 aaacgataat agcaagtttg ttctattaga ggaattgggt agagaatctg ttggaggaat
     4921 aggatttgct agtgatgatg taccagtagt tttggaagca acagaatctt ttggcatgtt
     4981 gaaaaaaata aaaccattta tagcaggatt tgcagagata gaattagata aaaatacagg
     5041 cgaatataaa gtcataaatt ttgcatgtgt acctgattgt ggaacagtta taaatccaac
     5101 tttagctaga atacaagtag aaggtggtgt gatgatgggt atagggtttg caatgtatga
     5161 agaccctata tttgataaag atggtagatt attaacagat agtttcctac agtataaagt
     5221 tcctacaaaa aaagaagttg gaaatatatt ggtagatttt gctgagaatt tcgacccagg
     5281 aggtccttat ggtgctaaat cattaggaga aatagttata catacaccag caccagctat
     5341 agcaaatgca atatttaatg ctacaggagt tcgtttaaga gagcttccta tgacttttga
     5401 aaaagtttat atgggtatga aaaataataa taaacaatta taataaataa taagatgtat
     5461 aaaagcaagt aaatttaaat tattttgtaa aataaagtaa ttaaatggac attatatatg
     5521 gtgttttgta aaaataccgt atataatgtc tagtagagta tattctaaag gaggtgagtt
     5581 catgtcaaac agaataaaaa atatacacta tggaactcag agacaacaat taaaaaactt
     5641 aactaaaaaa gaatatcaag ctctacgaga aatgtgcttc ttagctaaga acttatataa
     5701 tgttggtaca tataatgtaa gacagcatta tttcaatcaa ggagaacatc ttaactatga
     5761 agctaattat catgtatgca aagaaaatga gaactataag cttctcaata gtaatatagc
     5821 gcaacaaata ttaaaagaag tagacagttc gtttaggtca ttttttggat taattaaatt
     5881 agccaaagaa ggaaaatatg actttagagg gataaaatca cctaaatatt tagataaaga
     5941 aggatttttt agcataataa taggtcaaat aagaatcaaa gaagatggga ttttaaatgt
     6001 acctatgtct ccagcattta aaaaactata tgggaaagta agtataaaag ttccacaaaa
     6061 tatactagag aaaaaaatta aagaaataag aataatacct aaacataaag ctaggttctt
     6121 tgaaattcag tattgttatg aaataccaaa atcagaggaa gtttcaaatc aaaaaaatgc
     6181 actagctata gacttgggtt tagaaaattt atgtacatgt gttaccaatt taggtgatag
     6241 ttttatcata gatggaaaaa ggctaaaatc tgtgaatcag tgggcaaaca aacagaatga
     6301 taaaatagaa gccttaaaat taaaaaagaa tataactgca ataacaagaa aacaatttaa
     6361 agtttggaat aagagaaata atcaagttaa agactatgta aataaaacct gtttttatat
     6421 aattgattat tgtgtaaaaa ataatattgg aaatttaatt gtaggatata atgctaagtt
     6481 agaaaaaagt gataatatgg agagaaaaat atatagaaat tttataagaa tcccatttgg
     6541 agagataaaa ggaaaattag aatatctgtg taaaataaaa aatatcaatt ttattaggca
     6601 agaagaaagc tatactagta aatcagattt ttttgctgat gattttctac ctgatatcaa
     6661 tgtggacaat gttaaaaaat atgactttaa aggggaaaga ataacgcgtg gacagtacaa
     6721 atccagtgtt ggaatcatta taaatgcaga tgtaaatgcg agtcttaata tactaaaaaa
     6781 atcacaagta gttaatttat ctaatttaca gaataatcct caaatattaa agcaaccaca
     6841 gagagtaaga atatcttaat tatatttaaa agataaactg atagcgtaag cttacagctt
     6901 agcggcgaat tggatccgcc ttgttgttta caacagaatc tatttacttt cgagtttatg
     6961 gaatttcaaa aatcactttt actaaaaagg tctaactata ataattaaga gattagaaaa
     7021 ctggttataa gaggtgaaag tgtggaacta tcaaatataa tagaaaagaa tgaaataata
     7081 actgtagttg gagcaggagg aaaaacttcg cttataaact attttgcaaa tttttataga
     7141 gataaattga aagttctcct tacaacaaca actaaaatat atgtaccaaa cgattatgac
     7201 aatataatta ttacaataga tggaactgtt attccatcta tttgtcatgg aataacagtc
     7261 tgtggcagtt atataaataa tgaaaataaa ttagtaagta ttgattcctc tattttggat
     7321 gaaattgtag accaatttga tttagtttta atagagggag atggctcaaa gagaaaaaaa
     7381 cttaaaggtt ggaatgctaa agaaccagta gtatatcata aaactacaaa aaccataggg
     7441 atattagata taacatcatt tggaatgaat attaatgaag agaatataca tagagtagaa
     7501 atatttaaaa aaatagcaaa tttagataca agtagtatta atagtagttc tacagtaagt
     7561 attgaaaact taaaaaatat agtattaaat ccaaatggat tatttaaaaa ttattctgga
     7621 aaaagagtat tatttattaa taaagttgaa aatgaaaaat ataaaaattt agctataaaa
     7681 ttaatagaaa atattaaaga atatgaaagt gaaatagaga tattttatgg aagtgtaaaa
     7741 caaaaatttt gtgtaagata ttgaagtatt aaaatagttt atatgatatt atatatatag
     7801 ataatatcaa tagagatatt ataaaaatca tagaactatc aatatattat aaataccaag
     7861 gatgatatat atgataaatg cagtgataat ggcatcaggc tttgccagaa gaatgggagc
     7921 aaataaactt ttattaaatt ataaaggctg ttctataata gaacatgtat ttagagaagt
     7981 tagcaagatt gattttcatc aagttgtagt agtaagtcaa tatgaagagg ttttgttatt
     8041 gtgtaataaa tatggttttg aatatataga taataaaaat gcaaatatag gtcaaagtga
     8101 atctataaaa ttaggagttt taaatagctc aaaatgtgat ggttatatgt tctttgtagg
     8161 agaccagcca tttattgata acttatatat agataaaatt ataaatactt ttaaattaga
     8221 tagagatttt atagtaattc caagatacaa agatatgtgt ggaaatccag tcatattccc
     8281 atatagcaag aaagaagaat tgttggcact taaagaagat gaaaaaggta aaaccatttt
     8341 aaaaaattca tcaaaaataa agtatgtaga agttcctaaa gaaatgttat ttgatatcga
     8401 tacaaaaatg gactatgaga gaattggggg taaattatga agctaataaa aactatagat
     8461 gctgttggac aagttttatg tcacgacata actcaaataa tacctggaga atttaaagga
     8521 agaaaattta aaaaaggcca catagtaaaa gaagaagata ttccagtgtt attatctctt
     8581 ggtaaagata atttgtatgt atgggaaaag tcagaaggaa tggttcatga aaatgaaggc
     8641 gcattgtttt taaaagaact aactgctgga gaaaatttag attttagtga aataaaagaa
     8701 gggaaaatag attttatagc agcatgtgat ggtctattaa agatagatgt tgatgcactt
     8761 tttgatttga attgtgttgg agaaataatg atggctactc ttcataataa ttttatagta
     8821 aataaaggtt taaaagtagc tggaacaaga gtgatacctt tggttataga tgagaaaaaa
     8881 ttagaagatg caaaaaaagt agttggtaat agaaaaatag taaatgtagt accttttaaa
     8941 cctaagaaag taggaattgt tactacagga aatgaagtgt tttatagcag aattgttgat
     9001 aaatttggac ccgtaataga agaaaaagta aaaggctttg gttgtgaagt tataggacag
     9061 actatatgcc cagatgataa agagattata aagagtgcaa taaaagaatt tataagtcaa
     9121 ggagcagaac ttatctgttg tactggtggg atgagtgtag acccagatga tgttacacca
     9181 acagctataa aagaaactgg agctgattta gtgacatatg gttcaccaat attaccagga
     9241 gctatgtttt tacttgctta ttatggagaa gttccaatta tgggtatacc aggatgcgca
     9301 atgtatcaca aaactacagt atttgatata gttttatcta gggttttaat tgatgaaaag
     9361 cttgataagt atgatatagc tagatatggt catggtggcc tttgtatgaa ctgtgatgta
     9421 tgtacttatc ctgcatgtaa ttttgctaaa atataaagtt attaaaaaag agtatttaca
     9481 aattaagtac tctttttaaa tatgaaacaa aacagctaga atgatgtttt aagaaaagca
     9541 tatttattta gaaattataa taacttattt tcaatgtgta aaagaatgaa taaagttgca
     9601 atttgatttc tttataagta taatataaga gattgttaat ttttataaat ttacaatttt
     9661 tagtattatt ttaataaata tttcacaaat ttagtataaa aaatatacta tttacgtgat
     9721 aatatataat aaaaatgtta tcaaagtttt atgataaaat aaattatgag caaaaagaaa
     9781 ggaaaatata ttaatgttac aagttacagg tgttggactt agatttggtg ataaggaact
     9841 atttaaagat gttaacttaa aattcactaa aggtaattgc tatggaataa taggagctaa
     9901 tggtgcggga aaatctactt ttttaaaaat attatcagga gaaatagagc ctaatacagg
     9961 tagtatatct ataactgata aagaaagaat gtcagtttta aaacaggacc actttgaata
    10021 tgaagaagaa actgttttaa atgttgttat aagaggtcat gagagacttt ggaacataat
    10081 gaaagaaaaa gatgcattgt atatgaagga agactttaat gaagaagatg ggataaaagc
    10141 tgccgaactt gaaggtgagt ttgcagagct tgacggatgg gatgcagaaa ctaatgctga
    10201 gaaaatactt atgggccttg gcataacgaa agatatgcat tacaaacaaa tgaaggaact
    10261 agttggtggg gaaaaggtta aggtattgtt agcacaatca ctttttggta agcctgaaat
    10321 actattaatg gacgaaccta caaaccactt agattttaaa tcaataaact ggttaaataa
    10381 ttttataatg gatttagaag aatctatagt tataatagta tcacatgata gacatttcct
    10441 aaatcaaata tgtactaata tagttgacgt agattttggt aaaatacaaa tgtacgttgg
    10501 aaactatgat ttctggtatg agtcaagtca attggcttta caattggcta aggaccaaaa
    10561 taaaaagact gaagaaaaga tagctcaatt aaaggaattt atagctagat ttagttctaa
    10621 tgcttcaaag gctaaacaag caacatcaag aaagaaacag ttagagaaat tagaagttga
    10681 agatatacaa ccatcaagaa gaagatatcc atatgtagga tttacacctg caagagaaat
    10741 tgggaatgaa gttttagaag tacacaattt aactaaaact atagatgggg ttaaggttct
    10801 tgataatgtg tcatttagat tggatagaga tgataaagta gtatttatgg gagatgaaat
    10861 agctactact gcacttttca acatagttat gggagaactt gaaccagaca gtggagagta
    10921 taaatgggga gtaactactt ctcaagatta tcttccaaaa aatcataata agttttttga
    10981 cggagtagaa tattctttgg ttgactggct tagacagttc tctgaagaaa aaagtgaaag
    11041 ttttataaga ggatttttag gtagaatgtt attctcaggt gaagaagcat taaaagaagc
    11101 tcaagttata tctggaggag aaaaggttag atgtatgctt agtaagctaa tgctttcaaa
    11161 tgcaaatgta cttgtattag atgaccctac taaccactta gacctagaaa gtataacttc
    11221 tgtaaataaa ggacttgaaa agttcccagg agtgctttta tttacttctc atgaccatga
    11281 atttatatct actatagcaa atagaattat agagataact ccaaatggaa taatggatag
    11341 aaaaatggac tttgatgagt atttagaaag taaagagata caagaccaat tagctaaaat
    11401 gtatggaaaa gataaataat tattagtata aaacctttta atatttgaga catgctatgc
    11461 atgagtgtgt aaatttttaa tcaaatatta ggaggttttt tatgttgttt aaacatgttt
    11521 attagcagaa tagagtaata ttttgtatat taagaattta aatataatat agtagatgat
    11581 ataataaaaa tatataacat tttgggggga atagattatg gttaagaaaa ggttagtagc
    11641 tgtagtaatt agtttagcta tgattagtgt agggaatttt tcatatgctc actcaggagg
    11701 gcataattca tcaggaggtc atcataataa aaataaatca gagttaacac gttattattg
    11761 tggaggacat tcagcacatc aacacaataa aggtttttgt ccttatgcta gtacaaaaga
    11821 aaattctgga gtaacaatat cttcagtaca taacgaaaaa aatcataaaa actattttgc
    11881 agaaaaaggg tataataaag gttatgaaga tgggtgtaaa gggctttata atttaagtgt
    11941 cacagaagcc aaatctgata atttatatga aaaagagatt ttaaaaagtt catatgaaaa
    12001 aggatattta attggataca atgaatataa agcaaaaaaa gtagatgatg taatgaagaa
    12061 gatgttagat attccaatgt tagcaagtat ataataatat agtatttaaa aagatatttt
    12121 ctataaggga atatcttttt tattgtaaag tataattttt cgtaaaaaag tataataact
    12181 gaggcgataa attaaaataa aacagattat ttaagtagtt attaatttga gatagataca
    12241 tttaatatta atgaatctat ctcaataagc taagctaatt taaagctaag ataaattaat
    12301 attaattagc attaaataaa ctttcagaaa ttttgtcaga agcctcttta tccatttctt
    12361 taagtacatg agaataaacg cttagagtag tacttatatt tgagtgtcct actctctcgg
    12421 atataacctt tataggtact ttagaattta taagtaaagt aacatgtgag tgtcttaaat
    12481 catggaatct aatatgaggt aattcattct tttctaaaaa tcttttaaat tttttactca
    12541 taacatcttc agcaataggt tcacatttct tattgaaaaa caacaaatta ttattatttc
    12601 taatggaaga ttttaataaa tttttattct gttttatttt atattctttt aataaagaca
    12661 ttaattcttt gggagaagat atttttctaa cagagctttc tgtctttggc tctttgagaa
    12721 taacagaacc attaattcgg gaagttatct tatttactgt tattgtattt tcttcaaaat
    12781 caatattatc ccaagttaga cctaaaactt ctgaaattct aagacctaat ccaatagcca
    12841 aattaatagg taattccatt tctgtatttt ttgctaaatc taataatttt atcatttgtt
    12901 ctctatcata aatctcattt ttaaattttt tatatcttgg agcctctata ctatccataa
    12961 cattttcttt tattaatttg agtctataag ctctttttat tgcaagtttc aatatattta
    13021 tatgaatctt aattgattga ggatttagtt tataagatag tttatctatg tagttttgta
    13081 tgtgaaccgg atgtaagtct tgtattttat attttccaat agatggatta atgtaattct
    13141 tacaaatact aacataatta ttatatgtag ctaaagatat attttcacta tatttattta
    13201 gaaaatctaa tagaaattct gacactgtaa tttcgcttgg tactaaaaaa ccatcattat
    13261 ataagctttc ttttacctca tttagtcttt tacttgcttc ccttttttta tcaaaagtac
    13321 ccatattttt ttgttttctt ttacctgtct tttcatcttc aaattccagg taaacaacat
    13381 agtttttatt tctttttcta ataaatgcat tcatatataa cccaccaatc aaaattttat
    13441 acatttatta taacatatat atatcacaaa ataattttga aatagagt
//
LOCUS       DAEMGG010000106        13235 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEMGG010000106 DAEMGG010000000
VERSION     DAEMGG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.107"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            114..1973
                     /locus_tag="KPK24_002850"
     CDS             114..1973
                     /locus_tag="KPK24_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2300503.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2265..2864)
                     /locus_tag="KPK24_002851"
     CDS             complement(2265..2864)
                     /locus_tag="KPK24_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2300504.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2967..3764)
                     /locus_tag="KPK24_002852"
     CDS             complement(2967..3764)
                     /locus_tag="KPK24_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2300505.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3877..3982)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4074..5060)
                     /locus_tag="KPK24_002853"
     CDS             complement(4074..5060)
                     /locus_tag="KPK24_002853"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG2300506.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5544..7274
                     /locus_tag="KPK24_002854"
     CDS             5544..7274
                     /locus_tag="KPK24_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG2300507.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7384..7464
                     /locus_tag="KPK24_002855"
     tRNA            7384..7464
                     /locus_tag="KPK24_002855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7418..7420,aa:Leu,seq:tag)
     gene            7799..8314
                     /locus_tag="KPK24_002856"
     CDS             7799..8314
                     /locus_tag="KPK24_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBG2300508.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8806..8910
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9120..11594
                     /gene="gcvPA"
                     /locus_tag="KPK24_002857"
     CDS             9120..11594
                     /gene="gcvPA"
                     /locus_tag="KPK24_002857"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG2300509.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYIATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11594..13051
                     /gene="gcvPB"
                     /locus_tag="KPK24_002858"
     CDS             11594..13051
                     /gene="gcvPB"
                     /locus_tag="KPK24_002858"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG2300510.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         4959 a         1570 c         2185 g         4521 t
ORIGIN      
        1 tatatattct aattgtatag aaaatttatt gtaaacgcat gtatattatt agaaaaaata
       61 gtttgaaggt attcaataaa cataaaaaac aatatagggg ataaaggtgt tttatgaaga
      121 gatttttgag gagaattatc ttagttttat ttattttgtt gatttttata agttttattt
      181 ctattaaatt aattcataat gttggagatt atggaaaatt gattaattat gttgggattg
      241 ttcgtggagc tagccaacgt ctaactaaac tagagatgaa tcatcaacca aatgatgaat
      301 taatagaata tattgatgaa atcctacagg agttgattac agggcatgga gattatggtt
      361 tagtaatcac tgattgtaat gaatacaatg aggacttact tttattagaa aaaaagtggg
      421 aagatttaaa tattgaaatt aaaaaagtaa gaatgaaaga acagaataac cagttattaa
      481 gtataagtga agagtttttt tctcttgcaa atgatacagt gtttaaaata gaaaactttt
      541 ctaaagaaaa aagtaattac ttaatgactt taataataat tatatctatt ataggtatat
      601 tagcatgtat catattgatt ttacaatata gtaaaaaaat ggttagactg gaaaaattaa
      661 atgtagattt aaaaaatatt gcatataaag atgaactaac aggagtaaat actatagaaa
      721 aatttaaatt ggatgcaaat caaaatattt gtatacataa ggataagaaa tttgctgtat
      781 tctatattga ttttgaaaac tttaaataca ttaatgatat atttggatat gattatggtg
      841 acatgatact gaaaagatat gctaatctta tgatgaatga catagggaaa tatgaaatat
      901 ttgcaagaga aattgcagat agatttgttg cgttaagatg ctatgtagat aaggaagatt
      961 tagtggtaag acaacgagat gtagacaatg aactaattaa tataacaaat gaaattaaaa
     1021 ataaacatag tattactgtt gttagtggaa tttgttgtat tgaagatgta aatgaaaaat
     1081 taagtataga tggtttaata aatagagcaa attttgcaca aaaaactgtt aagaataaac
     1141 caggtactaa ttatgctttt tataatgaca gtattcgtaa aaaaatgatt gaagaaaata
     1201 ctatcaagag tagaatacat gaagctattg aaaaaagaga atttatagtt tatttacagc
     1261 ctaaagttaa tttacatagt caaaaaataa attgtgcaga agctttagtt cgttggctta
     1321 ctccggataa aggtattata tcgccagcaa tatttattcc agtacttgag aagaattttt
     1381 ttattgcctt agtagataag tatgtttttg aggaggtttg taaatggatt agaaagaggt
     1441 tagatgaaaa taaacctttt gttcagatat cagttaatgt ttcaagaata caattttata
     1501 acactaaatt tgtagaaact tattccaata tacaaaataa atacaggata cctaaaaata
     1561 ctatagaaat tgaatttaca gagagtgttg cttttgagaa tcaaaaacat cttttggaaa
     1621 ttatacatga tttgcatgaa aatggattta cttgttcttt agatgatttt ggaaaaggtt
     1681 attcgtcttt aagtgtttta aaagatttac catttgatgt tttaaagctt gattctatgt
     1741 ttttcaaggc tagtttagat aaagataaag agaaaatagt aataaaaaat attgtacata
     1801 tgttaaaaga acttaatatc acaactgttg ctgagggcat tgaatgcaag gaacaagttg
     1861 aatttcttag ggatattggg tgtgatttag ttcagggatt tgtattttat aaaccaatgc
     1921 caatattaga atttgaaaaa atattggata aagagtttgt ttataatagc taaaatgtac
     1981 tttataaaat atgaactaat taagtaaaat tattttgtac tgaataaatt tttactttat
     2041 ttacagaata atgattattt acagaaaagc gcgaaaagtg tattccttta agtgtgggat
     2101 ggatagcaca aatagaggag aaaaaatatt ggccatataa gaatgcataa taatataaat
     2161 atttgaaagg tcagcattat ataaaaatta aaaagaaaat aaataatttt tctataaaaa
     2221 agagactatg agaaaattta ctcatagtct cctaaataat gtatttattt tcttcctgca
     2281 acccatttta atccccaatg aaattcagta tccatttctc tatgaccttt gaagtattta
     2341 actacttttt caacactgaa tgtctcatca tttacatttt ctatcaatgc tattgaacac
     2401 atattatagc catttttatg ttcatcaagt ttgacaacta agtctgaatc ttgtttttgt
     2461 ttgattgtta caattccatc agcttcagac cagttagcta caccttcgta tataaaagca
     2521 taaataagta ttctttttat atcttttatt ttattaccat ttattcttaa attttctcct
     2581 tgtgtattac ttcctgttct gtcatctcca tctaattgag caaaaggtgg attgtctaaa
     2641 ctaccaaagg catttcctaa tgcttgaaca gctcctttta caccattttt catctcaaat
     2701 aggcaaccca aatctaaatc tatattactg cttcttaaag agccaaaaaa accctttttt
     2761 tgtacttttt gattccaatt taaattaact aagatttcac ctaaatcgct attttccttt
     2821 tttgtaaggt ttattttttg tccctttttt aattctatag ccatagtaat cttcctccac
     2881 aaacatagat ttatttaaat ggtagcactt aataagtcaa tattcaacaa gattagaata
     2941 ataatattaa aattatacta aatatactaa aaagtaggaa tcacaatacc tttgtatttt
     3001 tcttcaatta agcttcttgc tttatctgat gtaagaacat cttgtagaac tttaatcttt
     3061 tcactgtctt tatcttcagt tctaaatgct aaaagatttg catactcgtc atttatagga
     3121 gctgtataaa ttccgttttt cttggcatca agaccagcag gtacagcata agtagtatct
     3181 ataaatgcag cagtcacatc tggaagagaa gatggaattt gagcaatatc taactctttg
     3241 aattgtattc cctttggatt ttctattatg tccttaggac taactaatcc atccccttta
     3301 ggtaacgtta aaagtccaat actttcaagg tatctaaggt tcttagaaca agaagatggg
     3361 ttattgctta ttgcaattgt atctcctttt ttaaattcat caacagattt tatcttataa
     3421 gagtaaagta ttgctgggca tacatatagt tttttaccag ccattatttt gtaacctttg
     3481 gccttaacag cttcttttag ataaggttca tgttgaaata gatttgcatc aatttcacca
     3541 ttatttaaag cttcgtttgg aagtatgtaa tcatcaaagt ttttaacttc taaagtatat
     3601 cctttttctt taataagtgg ttttaattct tctaataact ctcctcctgg aactaaagta
     3661 gcaccaacaa caattttttt atcatcttta gctttagaac atccaaccac agctaaagtt
     3721 aatactagac ataataacaa acataataat tttttgaatt tcatataaat tccctcctga
     3781 atataattga ttagttaaaa ataaaatacc attctttatc acactgcaaa ataaaaataa
     3841 gcctgagttt aaactcaggc tttaaaagct aatttagact catctttcag tataatactg
     3901 caggatttag caccaatgta tataaaaata tactggttgc tgggtttcat agggccagtc
     3961 ccttcaccac tctggataag aagtatcaat ttttaattat gttataaata ttatcatgat
     4021 tttttacgtc tgtaaacata attttataaa tattttaaaa tctttaatgt attttattta
     4081 acacctaaaa ttccagaaac tagaatatca gtatcagcac ctaagaaata gtcatttatt
     4141 tcaatagaat ccagagtatt tctcaaagca ttttcttcat gccttatacc aataagttta
     4201 ttttctacaa atgcaacatc acattttcca aaaaaatctc caaaaaattt tatactagtt
     4261 attatgcctt tatcaacatt taaattaaac tcaacatttc caccagaata ttttaattca
     4321 tttgaaagag tatattttgg tgaattacca aaattccaat cccaagtttc gtattttgat
     4381 ttagtaagct tttctatttg ttttatatca tgctcactca aacaataatt agtattatca
     4441 gtatttgttt tagaaagata atccattata gcatctttaa attctaatat gtccattttt
     4501 gaatgtaaat gttcacttat attggttaca cgagatttta cagattttat tccttttcct
     4561 tcaaatttta taggtttaac ctttaatgct tttgagaggt catttatttc agatgaaaat
     4621 aaaagagtac catgatgcat tactttgttt ttgtagttgt attgagcatt tccagaaaat
     4681 ttcttgccat ctataagaag gtcatttctt ccagaaaatt cggcattgac atctagtgtt
     4741 ttaagtaaat ctattatggg ctgagtgaat cttctgaaat cactaaaatt attattgtta
     4801 caagctataa atgtaaaatt tatatttccc aaatcatgaa atacagctcc accaccagac
     4861 tgtctcctaa cgacaggtat agaatattct tttatatatt ctaaatttat ttcagaaagt
     4921 gtgttttggt tttttccaac aattatagaa ggttcattcc tccataaaat aaataagtct
     4981 tcatcaaaat tttttagaag atattcttcc attgctaaat taaaataagg gtttgttttt
     5041 tcattataaa ttaataacat taaagtagtt cctccataga attgataaaa tttttattat
     5101 gaatacaatt aattttataa ttcgacaata atataaaaac tcctttaaat tagaaatttt
     5161 aaaaactacc ttgcaaagtt taaataaata atatatactc aaaatatgtt taataaaagt
     5221 gaatagaagg gtagaggcgc aataatctat cagtactaat aaggagttaa gcacagagat
     5281 ttattaggaa aggagatatt gccgaagttt tataatcgat gctttaagat tatattgctg
     5341 ggggtacata gaaaatatgt acaactgtca caggtttatt tgtggtgagc tatcatttaa
     5401 ggaattatat tatatttatt tttactattg ttgatatatt gtaatgttta agccttgagt
     5461 gtttaccact caaggctttt ttagttttat tgtaacataa taccctgtac agttcacaaa
     5521 attaaaagga gagggagatt tatatgagga acaaaaaaat taatattatt tttctgacga
     5581 caatcatgtt cataatgtca acagtaatgg tttttgcaga agaagatata gacactattg
     5641 cactagccaa tgcagaaaaa tttgggattt taactttaat accaccatta gttgctataa
     5701 tacttgcatt tataactaaa aacgtaataa tttctttact tataggtatt ctttctggta
     5761 gttttataat taaagctagt ggtattaatg tttttgcgac atttatacag gcatttttag
     5821 atttagtaga cagagcttta gtatctttag cagacccttg gaatgcaggg ataatactac
     5881 aggttttggc aattggagga gtaataaatc ttgttgctaa aatgggagga gctaaagcta
     5941 tagctgaagc acttgctaaa agagcaaagt cagcaaaagg gacacagctt ataacatggt
     6001 ttttggggct tttagtattc tttgatgatt atgccaattc actaatagta ggtcctatga
     6061 tgagacctgt tgcagataaa atgaaaatat cgagagaaaa gctagcattt ataatagatg
     6121 ccacagcagc accagttgct ggtttagcta taatatctac atggataggt cttgaggttg
     6181 gtttaataca tgatgctttt gaaagtataa gtatagatgt agatgcattt ggtatatttt
     6241 taaatacaat accatttaga ttttacaata tattaatatt agctttcatt gtaatatcag
     6301 cattattatt aaaagaattt ggtccaatga gaaaagctga gataaaatct agaagtagaa
     6361 aaattagtat agaccttgat gaaggcgtag aagagttgga tgatttagct ccaaaaaatg
     6421 gagtaaaatt gagtgtatgg aatgctataa tcccaatagg aacattgata atagttgcat
     6481 tagcatcatt ttattatagt ggatatacat ctataatggg aggagacgat aaggctctga
     6541 tacaactatt tacaaattct ccatattctt ttgaagcaat aaaagaagca tttagtgcat
     6601 ctgatgcatc tagagcatta ttccagtctg cacttgttgc aagtttagtt gctattataa
     6661 tggctgtagt aaaaaagatt tttacaatat cagaagcaat tgatgtttgg atagatggaa
     6721 tgaaaagttt agtaatcact ggtgtaatat taatacttgc atggtcatta agttcagtaa
     6781 taaaagagtt aggtacagct aaatttttaa tacacctatt atcaggttca ctaccaccat
     6841 tcttattacc aagtttaata tttggtttag gagcaataat atcttttgct actggaacag
     6901 cttatggaac aatgggtata cttatgccac ttgcaattcc attagcatac tcattaaatc
     6961 ctgatatgtc ttatgtaata gtaagtacta gtgcagtttt aacaggtgca atatttggag
     7021 accattgttc tccaatttca gatacaacaa tactttcttc aatgggagct ggctgtaatc
     7081 atatagacca tgttaacact caaatgccat atgctatatt tacagcagta atcactattg
     7141 tatttgggta tataccagct ggacttggat tgccaatata tatagtatta ccagtggcaa
     7201 tagctgctat atttgtaggt attcaaataa ttggtaagaa agttgatgaa gcggaaatag
     7261 aacttgtaga ataaagatat aaataagttt caccttttat tatcactatt ttgttagttt
     7321 ttagataatt ttaaaaagtt attgaaaata atggcgaaat atagtaaaat aaaagagtga
     7381 taagcggatg tggcggaatt ggcagacgca ctagacttag gatctagcgc cattggcgtg
     7441 ggggttcgac tcccttcatc cgcactttac agtacttaat tgtactgaaa aaaactgggg
     7501 ttgctggctt tcggagtggg ctatcccgtt tttatttttt tgtatatata atagattggc
     7561 actttagtat aaggtgcttt ttttataggc aaaaacagta catttatttc tgttttatta
     7621 tcattcatct attagaatat actttagtta agaatgtgtt aataagcagt gttcacattc
     7681 ttgataaaat gtggaataat atgtattttt actaagacgg aaatgaaata attaaaccat
     7741 taaataatat tataagatga tgaataagta gataaatcaa attttgtagg aggatattat
     7801 ggaaaaatat atatggttgt ttccattact ttttattttt catgatatgg aagagataat
     7861 aggttttgga atatggctta aaaaaaataa atcaatgctt gataagaagt atccatttat
     7921 cagtaaagtt tatgaaaatt atagtactga aggcatggca tttgctgtgt ttgaagaatt
     7981 tattttatgc attattttct gtatacttac agttataaca gagaatcagt atgtatatct
     8041 actatggtta ggtagtttta tagcatatac cttacatttg gtaattcata tagggcagtc
     8101 aatcataata agaaaatata taccttcact tattaccagt atcatatgct taccaataag
     8161 catttggtgt ataagtaaga gtatttatat agttgattgt gaaatgagta caacaattct
     8221 gtacagtata attggcatta taattgtagc attgaattta aagtttgcac agtcgctgat
     8281 tggaaaattt acaaagtgga tgtcaaacat ataaatttta gtttattgga cagttcaagg
     8341 aatcagaaat tggaatttta tagtattaat attttatggt attaatataa tgtataaaat
     8401 tttatagcaa tgtatttcat aagtataaag tgactagaat tttaagaaat atacaaattc
     8461 tataaaaact aaataatttc aagtatattt taaaaatctt atttttcaca agtttaaaat
     8521 tgtaaacgat ttcatcaagt ctatgaatat acttttaaca tgttatagta aatctcaata
     8581 taaatttaca tttggttctt tgtgtgttat tttaataata aattaagtga aaatgttatt
     8641 ttacattaat atataatatc tttgcataag agtgaataat ataaaagaga aataattgat
     8701 aataaaaact taacaaaaaa attaatacat aaaaaaaaat aaatataatg tgttgacatt
     8761 taattgaaat attcatacaa taaaattgaa aataaatatt tccaaatgag attagcgaga
     8821 gagtccttta tacgaaaagg cgccgaagaa gaaatttaag aaatgccaat ttttagagaa
     8881 gctttcaggc aaaaatatcg ctaattgatg ggactctgga gagacttaag ttaagatact
     8941 ataagtcacc gaagaagcaa aataatatta aattattaaa tctttcaggt atacaaacag
     9001 ggatatatag gctaggatta ttttccatgc tgatatatct tttttttatg aaaaagtaca
     9061 ggaaataata tcaaagttac ttaaatacag taggtctaat tttctaggga gggttatata
     9121 tgaatgaatt gaaaagagta tctttgtaca atatacacaa agagcttgga gcaaaacttg
     9181 ttgaatttgc tggttgggaa atgcctttag aatatgaggg tataaataaa gagcatgaga
     9241 aggtcagaaa aagtgcaggt atatttgatg tatctcatat gggagaggta cagataaagg
     9301 gagcagagtc agaaaaattt atacaaaacc tagttacaaa tgatataagc gcattaaaga
     9361 taaatgatat tatatataca cctatgtgtt atgaaaatgg tggagtagtt gatgatttat
     9421 taatatataa atttggtgaa gaagattatt tattagtaat aaatgcaggt aatatagata
     9481 aagatgtagc atggataata aaacaaagtg aaggatacaa tgtagatata aaaaatatat
     9541 ctagtgaagt gagtcaatta gcaattcaag gacctaaagc agaagaaatt cttcaaaaaa
     9601 tcacagatat agacttaaac tcaataaaat tttataagtc aattccatca acaaaagtat
     9661 gtggttgtcc ttgtttagtt tcaagaacag gatatactgg ggaagatggg tttgagatat
     9721 actgtaaaaa taaatatgta gaaataattt ggaatgaagt tctaaaggtt ggaggagaag
     9781 atatatgtcc agctggtcta ggttgtaggg atactttgag atttgaagca gctctacctc
     9841 tatatggaca tgaaataaat gaacacatat ctccaattga aggtggttta tctatctttg
     9901 tgaaaacaaa taaagaatca tttattggca aatcaatttt atcaaaagaa aaagaaagtg
     9961 gagctaaaag aaagttagta ggatttgaaa tgcaaggtaa aggaatgcct agaaatggat
    10021 atgatataag gattggtgat aaaacagttg gatttgtaac aacaggatgt gcatctccaa
    10081 cgacaggaaa aattttaggt atgggaatta tagattctga atatgcaaaa gtaggaaatg
    10141 aaataggtat agcaatcaga aaaaaagttg ttcctgctgt gatagttaag aaaccttttt
    10201 ataaaaaaca atataaaaaa gataatatta tattaaacaa agaaaataaa ttttcatata
    10261 tacctgctac aagtgaagat aaatcaaaaa tgttaaaagt agttggatta aattcggttg
    10321 atgaattatt ttcagatata ccagaagaag taaaattaaa aagagattta aatttggaaa
    10381 ttggaaaatc tgaattagag gtaagcaaaa tagtaaaaag attgtcagaa gaaaatttaa
    10441 gtttagaaga cttgacttgt tttcttggtg ctggtgcata tgaccattat ataccatcaa
    10501 ttataaaaca tataacttca agatctgaat tttatacagc atatactcca tatcaagctg
    10561 aaataagtca gggaacttta caagtagttt ttgaatttca atctatgatt gcagaaatta
    10621 caggtatgga aattgcaaat gcatcaatgt atgatggagc aactgctgca atagaagcat
    10681 gtataatggc aatgaatcag actagaaaaa gtaaaatagt agtgtcaaaa actattcatc
    10741 ctgaaacttt gtcaatatta agaacttact tacaatataa agattgtgag atagtagaaa
    10801 ttgacttttg taatgaatat ggcactacag atatagaaaa attaaaagct tctgtagaca
    10861 aagatacagc atgtgttctt atacaaactc ctaatttctt tggaattatt gaagaaatgg
    10921 aagaaataga aaagataact catgaaaata aggctatgtt aattatgagt gtagacccaa
    10981 tatcattagg agttttaaaa acaccaggtg agataggtgc agatattgtt gttggagaag
    11041 ctcaatcact tggaaaccct cttaattttg gaggtcctta tgtaggattt ttagctagca
    11101 aatctaaata tactagaaaa atgccaggaa gaatagttgg gcaaagttta gatgtagaag
    11161 gtaaaattgc atatgtatta actttacaaa ctagagaaca gcatgtaaga agagaaaaag
    11221 caacttctaa tatatgttca aatcaagctt taaatgctct tgtagcttca atatatatag
    11281 ctactatggg taaagaagga tttaaagagg ttggtatgca gtctatgaaa aaagcacatt
    11341 atacttacaa taagcttgta caaactggaa aatataagcc aatatttaaa ggtaagtttt
    11401 tcaaagaatt tgctgtacaa ggaaatctta atatagaaac tataaatgat aaactcttag
    11461 aagaaaatat attaggtggt tataatttag aatacaatta tccagaatta aaaaattcaa
    11521 ctttgctttg tgtgactgag aaaagaagca aagaggaaat agataagtta gttggaataa
    11581 tggagggatt ataatgaaag aatacaatag tttgttgata gatatatcta aaaaaggtag
    11641 gaaagcatac tcacttccaa agttagatat agatgatata aaaatagaaa atatgattga
    11701 ccaagatatg gcaagacaaa gtgagttgaa tttgccagaa gttggtgaat tagaattagt
    11761 tagacattat acattattat caaataaaaa ctttggagtt gatacaggtt tttatccttt
    11821 gggttcttgt actatgaaat ataatcctaa aataaatgaa gatatggcag cacttccaaa
    11881 ctttactgga atgcatccat accaatcttc ggatacagct caaggttctc tttctttgat
    11941 gtatgattta tcaagaagac ttgcagaaat tacaggtatg gatgaagtta cactacaacc
    12001 ttcagcaggt tctcatggtg agtttacagg gcttatgatt ataaaagcat atcatgaaaa
    12061 tagaggtgac cataagagaa ccaaagtgat aattccagat tctgcacatg gaacaaatcc
    12121 agcaagtgca gctatggcaa actttgatgt gattcaaata gcatctgata aaaatggagc
    12181 tgtagacata aatgcattaa aagaggtttt gaatgatgaa gtagcagctc ttatgcttac
    12241 taatccaagt actcttggtt tatttgaaaa aaatataaaa gaaatagcta ctcttgtaca
    12301 tgaagcagga ggacttttgt actatgatgg tgcaaacatg aacgctatta tggggataac
    12361 aagacctgga gatatggggt ttgatgtggt tcatcttaat cttcataaga ctttttcaac
    12421 tcctcatgga ggtggaggtc caggagctgg tcctgttgga gttaaaaaag aattagtacc
    12481 ttttttacca ataccagtaa ttgaaagaaa agaagataag tatgtactaa attatgatag
    12541 agaaaaatct attgggaaaa ttaaaaattt ctatggtaat tttggagttc ttgtaagagc
    12601 ttacacatat atactaacta tgggaaggga tggacttaaa gaggctagtg aaatggcagt
    12661 tttaaatgct aattatataa aggaaagtat aaaagatgat tatattttgc caatagatac
    12721 cttgtgtaag catgagtttg tattaggtgg attgccaaga ggagaggcca atataaaaac
    12781 tatcgatatt gcaaaaagac tactagatta tggataccat cctccaacaa tgtactttcc
    12841 tcttattata aatgaggcac ttatgataga acctacagaa agtgagagta tagagacatt
    12901 agatagtttt atagaagcta tgaaaagtgt tgctaaagag gcaaaggaag aaccagaact
    12961 attgaaaact gctccacata atactttggt caaaagagta gatgatgcaa gggcagtaaa
    13021 gaaacctata ctaacatggt cacaaagata aatagttaaa aagtagagat ttaaagtata
    13081 aagagaatta aatttgtaag aaaaagctat ctaacttaat ctagtttaga tagctttttt
    13141 gtatattagt atgtatggta ttttctatat aaagttttaa attatattag tgcttatata
    13201 gaatattgat atgatgatta tgtatagata ttaat
//
LOCUS       DAEMGG010000107        13135 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEMGG010000107 DAEMGG010000000
VERSION     DAEMGG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.108"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(108..581)
                     /locus_tag="KPK24_002859"
     CDS             complement(108..581)
                     /locus_tag="KPK24_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG2300511.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(584..988)
                     /locus_tag="KPK24_002860"
     CDS             complement(584..988)
                     /locus_tag="KPK24_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG2300512.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(963..1592)
                     /locus_tag="KPK24_002861"
     CDS             complement(963..1592)
                     /locus_tag="KPK24_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2300513.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMYSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      1957..2000
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2101..3069
                     /locus_tag="KPK24_002862"
     CDS             2101..3069
                     /locus_tag="KPK24_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG2300514.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            3071..3634
                     /locus_tag="KPK24_002863"
     CDS             3071..3634
                     /locus_tag="KPK24_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2300515.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            3642..4331
                     /locus_tag="KPK24_002864"
     CDS             3642..4331
                     /locus_tag="KPK24_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2300516.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            4458..5186
                     /locus_tag="KPK24_002865"
     CDS             4458..5186
                     /locus_tag="KPK24_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG2300517.1"
                     /translation="MMFDRVKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            5200..5874
                     /locus_tag="KPK24_002866"
     CDS             5200..5874
                     /locus_tag="KPK24_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2300518.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYIFKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            5900..6736
                     /locus_tag="KPK24_002867"
     CDS             5900..6736
                     /locus_tag="KPK24_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300519.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            6752..7423
                     /locus_tag="KPK24_002868"
     CDS             6752..7423
                     /locus_tag="KPK24_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300520.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            7413..8645
                     /locus_tag="KPK24_002869"
     CDS             7413..8645
                     /locus_tag="KPK24_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2300521.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            8795..9112
                     /gene="trxA"
                     /locus_tag="KPK24_002870"
     CDS             8795..9112
                     /gene="trxA"
                     /locus_tag="KPK24_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG2300522.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            9129..10007
                     /locus_tag="KPK24_002871"
     CDS             9129..10007
                     /locus_tag="KPK24_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2300523.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            10277..10645
                     /locus_tag="KPK24_002872"
     CDS             10277..10645
                     /locus_tag="KPK24_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2300524.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            10648..11019
                     /locus_tag="KPK24_002873"
     CDS             10648..11019
                     /locus_tag="KPK24_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG2300525.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(11164..11619)
                     /locus_tag="KPK24_002874"
     CDS             complement(11164..11619)
                     /locus_tag="KPK24_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300526.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(11658..12923)
                     /locus_tag="KPK24_002875"
     CDS             complement(11658..12923)
                     /locus_tag="KPK24_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG2300527.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         5170 a         1425 c         2084 g         4456 t
ORIGIN      
        1 cattagattt cactaagaaa aataaaattt tgataaagtt taaagactat tttgagcaat
       61 attacttata aataaaagaa tttatcatag aaataattag ttttatttta gctattaaga
      121 tttattaaaa aattatctat attatcctgt gatattctaa aactgtcata attacctttt
      181 gaaacagcct tcataataag tttatctaaa aatttcattt tttctaatcg agcttcacta
      241 ccaaaaattc caatagcatt tgcactttct aataaggatg atggaaaatt tgattttaaa
      301 tatttatctg cttgttcaga aaagccacag caaaggaaaa tgccaacttg ttttttgttt
      361 aataactcta cattatcatt acatagcact tgtatttttt ttgaaactga accaacatat
      421 atagaacttc caataataat tttatcaaat tttgataact caattttatt attattatta
      481 atatcaacaa tagtgacatt atctgatagt ttatttttta gtatatttgc acagtctttt
      541 gtacaaccat atttacttga atatataata atagtattca taattattct ccctttctta
      601 aagcattttc tacagctttt cgtatagtat tactgctata agttgcagaa gttatagcat
      661 ccacatctaa agactgttgt ttaataatac ttgttgtaat tttttcagca ggtttaccta
      721 aaagatggtc gtgttttaag atattaatat tttggatttt accattatta acttcaacag
      781 acacagtagc ttttaccaag ttattatcag cacttccagt ataaatgcca tttttaacat
      841 tattaatatc tatatctttt actaccaagt tttctggctt taagttgctt acaattacaa
      901 taataatgca tataaataca aagaatagag caatagatat ttttacatat tttttctttt
      961 tattagtagc cttgttgcga attttcattg taaagtcctc ccactaaaat atctaagaaa
     1021 ttttctataa gttgagaaaa attatgtaaa gaatatagtt tattttcaac aacacataaa
     1081 acgaatccta acaatgcact tataagcatc catgagcttt tatctatatt ttttttgaat
     1141 attccttgtt caattccaat ggaaaggaat ttatcaagca tttttatacc attattgcta
     1201 ggcgtattgt ttgtattttc agatgcaaat ataatattca ttctactata catgatagat
     1261 tttgccatct ctggttcact agaaagagtt tttataaagc tagtcataat ttctatgatt
     1321 tgtttatcta ttggtagtga tgagcaatta ttcataatgt caatggcatt actttctact
     1381 gtgttataaa gtttattggt catatcgctg ataatctctg ctttgtcttt gtagtaaaga
     1441 taaattgtag ttggagaata ttctattttt gtagcaattt ttcttattga taaattttca
     1501 tatccatgtt ggtttataat ttcgatgcta gcatccataa tctttttctt catttcttct
     1561 ttttcatttt tacgtctttc tttgatactc atataatcac ctctttgctt aacggtgtta
     1621 agttaattta cactgttatt gtataattta attttaatac tgtcaataaa ttattaaata
     1681 atataagtta atatattttt atggataata aattaatgtt attaattgta atatttaaga
     1741 tattattgta taataataat cggtttaaat gcgccacatc ttattaaagt tggttcataa
     1801 ttctaaataa actttaataa gatacattaa aatataataa attcttttat gctataaaat
     1861 ttattttata gctattaaat cagatgttta aaaatttttt aacagtctat agtttttaag
     1921 agtctatagt aagtagccta aaaatatgat tttacatctg tacttgttcg agaactccaa
     1981 gtataaaaaa ctaaggaatt atatagtggc agtatataat taataccctt agacacacct
     2041 ttttgaaagt ggaaaaaaga tgtgtagttc tcctcgacca caaatcaagg aggataaaaa
     2101 atggaaaaga gaaaagtaat aattgattgt gacccaggaa ttgatgattc tttggcaatt
     2161 cttctggctt taaactcacc agagctagaa gtaattggaa ttaccacatg ttgtggaaat
     2221 gttccagcaa atataggtgc agaaaatgca ctaaaaacac ttcaaatgtg ttcttcacta
     2281 aatattccag tatatatagg agaagaagca ccactaaaaa gaaaacttgt aacagctcaa
     2341 gatacacatg gagaagatgg tattggagaa aacttttatc aaaaggttgt aggagctaaa
     2401 gcaaaaaatg gagcagtgga ttttataata aatactttat ataatcatga aaaagtatca
     2461 ataatagcac ttgcaccact tacaaatata gctaaagcac ttattaaaga taagaaagca
     2521 tttgaaaatc tcgatgagtt tgtatctatg ggaggagcat ttaggattca tggaaattgc
     2581 tctccagtag cagagtttaa ttattgggta gacccacatg gagcagatta tgtttacaag
     2641 aatttatcta aaaaaatcca catggtaggt ttagatgtaa ctagaaaaat tgtacttact
     2701 cctaatatta ttgagtttat aaatagactt gataagaaga tggcaaagta tataactgaa
     2761 ataactagat tttatattga tttccattgg gaacaggagg gaataattgg ctgtgtgata
     2821 aatgaccctc tagcagtagc gtactttata gatagaagta tatgtaaagg atttgaatca
     2881 tatgtagaag ttgtagaaga tggaatagct atgggtcagt ctatagtgga ttctttcaat
     2941 ttctataaaa aaaatcctaa tgcaattgtt ctaaatgaag ttgatgagaa gaaatttatg
     3001 tacatgtttt taaagaggct ttttaaaggt tatgaagaca ttatagactc tgtggaagga
     3061 gtgatatagt gtgaaaagaa aaatatgtgt aaaaactatt acacttatag cttttggtat
     3121 ttctataaat ataataggcg cttttatagc tatgggatta agattacctg tatacttaga
     3181 ttcaatagga actataatga tagcttcact tttaggacct aaatacgctg ttgttacagg
     3241 tgtgtttgga agtttaataa gtggaattac gtttgatgtg tattctctct attttgcacc
     3301 agttcaaata tcaaccggat tacttgctgg aatagcattt aaaaagaatt ttttaagggg
     3361 tttaaaaact ccactaggag tacttttatt tgccattcca acatctataa taagttcaat
     3421 aatttcagca tttttatttg gaggtatgac ctcttcaggt tcatcatata tagtgcaaat
     3481 cttgaaagtg ttaggactgg gggatgtttt tagtgtgttt gtaacccaag tatttactga
     3541 ttatggagat aagctgttag cagttgtttt agtgaattta ggattaaatg cagttccaaa
     3601 aactttaaag gtatcattga ctggaggaaa atagatagaa aatggataga tatagtgaga
     3661 taacaaataa aaatcaaaga gaaattgttc tgcttaaagg atttccttgt atatggggaa
     3721 agtgttcttt ttgtgattat atagatgata attctaattt agaagaagag atgaataaat
     3781 taaatctaaa ggttttaaaa aacgtaactg ggaaatatgg agttcttgaa gtcataaatt
     3841 ctggaagctg ttttgaatta cctaaggata ctttagaaaa aataaagtgt attataaaag
     3901 agaaaaatat aaaaaaatta tttttagaaa gtcattggtc ttacaaaaat aggcttaagg
     3961 aaatgagaga gtattttgaa attcctgtgg tatttaaaat tggagttgaa acctttgaca
     4021 atgattttag aaataatatt ttaaataaaa atgcaaattt taagactcca caagatgtaa
     4081 aagaatattt tgattcacct tgtattatgg ttggaataaa aggacaaaca aaagagatga
     4141 tagataaaga tatagagatt atcctaaata cttttgaaaa agcgacagtg aatgttttta
     4201 taaataattc ttctagtata aaaagagatg aagagttagt gaaatggttt gttagtaagt
     4261 ataaattctt ggatgaaaat ccaaatatag aagttttata caataataca gattttggtg
     4321 taggagatta gcattaattt agtttaaatt taaaattgaa aagtttctta tgtttttgaa
     4381 aaagcttcta atagttagaa gctttttttg tagagtttta tgttacaata agaaaaaagg
     4441 aattaaggag caattaaatg atgtttgata gggtaaaatg gaataaattg gattataatg
     4501 aacttttaga atatatgatg tctatacaag atataaaata tagagatttt aataaaaagc
     4561 ttatacctgg aacagaaaat ataataggta ttagagttcc taatttaaga aagttatcaa
     4621 aagaaataag ccaagggaat tggaaggaat ttttagaagt agcagaggat acatactatg
     4681 aagaagttag gctacaaggt atggtaattg gcaatataaa tagtaatttt gaagaaactt
     4741 tatactatgt aaaaagattt atacctaaaa tcgataattg gtcagtttgc gatggatttt
     4801 gttctgattt aaagtctatg aaaaaatata aagaaaatat gtatgatata cttaaaaaat
     4861 atgtttattc aaagaatcca tgggaaatta gatttgcatt agttatgttt ttaatatatt
     4921 atgttgatga taaacatatt aaagaaatat ttgaatactg taataatata caaagtgaag
     4981 aatattatgt taaaatgggt atggcatggt tgctatcaat ttgttttatt aaatgtgaag
     5041 aggaaacttt tctctatata aaaaataata atttagatga tttcacttat aataaaatgc
     5101 tacaaaaaat aattgaatca aatagagtag atttagaaaa gaagaatata ataagaagta
     5161 tgaaaagaaa gacaagaaga tgttgacgga ggaatattaa tgaataacaa tgaagacaga
     5221 attgacaaat ttttagaact tcaaagcaaa ggatttaaat ttgcagagat agctcaaaat
     5281 ctaaagatta gtcaaagtac tttaagaact tttttaaata aaaaaggata taagtcagaa
     5341 aaaggtaaat acatatttaa agacgaatta aattataata aaagtttgga taatagaaac
     5401 ttggaagaag agaaagctaa ggagtatgaa aaaagtaaaa agaatactat aagtaataga
     5461 aaaaaagtaa gcaccaaaaa aaagatttct aatgaaaagg ctttaagtaa aagtgttgaa
     5521 caaattgagt ttaatgatac aggtttaaaa aatgcaaagc caaaacaaaa tattaagcct
     5581 aagaaagata gaaaaataaa tataactcaa gaagatttag ataagctttg tgaagtatat
     5641 gattggtatt tagaagtaaa agattacaat ataaagccta aaaaagtatc taataaaaaa
     5701 gatgtacata tagatgatga acacatagat gaattaaaaa caacaagaat acgtatagat
     5761 aaagaaatat ggaatgattt tgaaagatta tgtagtaatt cgaattttga aaaacatcaa
     5821 atacttacac aggcattgaa agaatttatg aaaaagtata aaaatctact ataaaatact
     5881 actatatgag gtgcttaata tgaataaaat agtaaaaaaa tcaatttctc taatgattat
     5941 attaacaata tttattttta tgttgacagc atgtgaaaaa gatgaacagc ctgattcagt
     6001 tgatattcaa acagaagata aaaatgagat aaaaatagat gagggaaatg caaaagtatt
     6061 gaatatagga aaatcagaaa ttataaatat agatgaaggt gataaagtag atacatcaac
     6121 aaaggtagaa aataattcaa catttaatat atccaatgtt gaattgatat ataatgaata
     6181 tgatgagaat aaaaaaatta tatcttcgga tagtaaggct ttgttggaca tgactttaat
     6241 gcctgggaaa gttgcttaca tagaatgtgg gcataaaact tttgtaaagg gtgtagaagt
     6301 ttatgcatat gaatatgagg cagagggtaa gattgtgtat gttaatttaa aagaaaatac
     6361 tattaatata agaaataata atattaagct agaaaatagt agtcagtatg aggtgctatc
     6421 tacatcagaa cttaaaaaag taaatgaatc aaatgaggga attacgtatc aagtaaaagt
     6481 aaaaaattca tcatcaaaag atttaggaaa tattattctt aagacagcag aagttaatga
     6541 aaatggagaa tatttaacag taaatcgtgt gccatcatat aagattttaa agccttcaga
     6601 agagacagat atagatatat tgtgttcaac aaaggcaaaa agtgttgaaa tagtgggata
     6661 tacgtatgat gatataaaag aaaaagccaa tgtggatatt gacttaaaat ctcataaagt
     6721 taagatagac aaataaaggt aggggatact tatgaaggtt ttaatagttg aagataataa
     6781 gatactttta gaaagtgtag tagaagaact tagcaagcat tttgaaactg aaaagtgtga
     6841 agatggagaa gaagctttat acttagtaaa tcaaaatata tatgatttag tcattttaga
     6901 cttaatgctt cctaatataa atggatttga tatcctaaag aaaatgagag ttaataatat
     6961 agatacacct gtacttatat tgacagcaaa agaaacatta gatgacaaag tagaagcatt
     7021 tactatagga gctaatgatt atttgacaaa acctttttat atggaagagt tagtagctag
     7081 ggtatacgct atattaagga ctaatggaaa gataaaagaa agaaacggat tagaatttaa
     7141 atctttatat ttagatacac tagaaaaaag agtgtatatt gaaaaagaag aaataaaact
     7201 tcaaaacaaa cagtttaact tgcttgagta ttttgtatta aataaaggtt caattttatt
     7261 aaaagagcag atttatgata gaatatgggg aattgattca gatgcgacta ttgaaatagt
     7321 ggaagtatat gtaagtaatc taagaaaaaa actaagtaaa tatggatatg ataaatatat
     7381 taaaactaaa cgtaaggtag gatatatatt cgatgataaa taagaatgta tttaccagta
     7441 ctaaaaatca tttaataaaa atgtatatta ttgtagtagg tagtttttta ataatctttt
     7501 ctatatttat atactcatat tttaggggac ttacatatag tggaattgat agtgagataa
     7561 atgatgaact tgaatatata gtctctcaat ttaaaagaac ttcatttttg aacccaataa
     7621 gactgaaaga tcctaaagat atggtgtatg tgtatgaaga tggaaggata agttactata
     7681 ctcaaaatga atattttgat gagttacttc cagatagaag actagataaa aaaaattcgt
     7741 tttttaaata cacagaaaat ggatatactt ttagagaatt aaatgtagat gtaggtagat
     7801 accaaataca gataattaga aatatagatt ctgagatgaa ttctttaaga cagcttacat
     7861 ctgttcttat aattggaata ttaatttctg taataattac ttactttgtg gctgtatatc
     7921 tcactagaaa agctttaatt ccaatagaaa cagcatggaa aaatcaagct aagtttatac
     7981 aagatgcttc tcatgaatta agaacaccaa taacaatagt ttcatcaaaa ttagaaagta
     8041 tgttaaaatc cccagaaagt acagtaaatg atgaagttga aacaatagct actgcaatga
     8101 aggaaactag aagacttaaa aaaatgataa ctgatttatt gtctcttact aaagaggact
     8161 ctattgttaa agttaattta gaagaaatag atttagaaaa gttattggaa gaaatatcag
     8221 aagactatat tgacattgct gagtttcagg aaaaaagatt cgtgtttaat tctaaactta
     8281 agaataaagt tataataaca gataaaaata aattaagaca gcttatatta attttcattg
     8341 ataatgcctt taagtataca aaattgggtg atgagatagc attagaattg aaagaagata
     8401 tagaggatga ggtaacttta cttataagtg acactggtat aggcataaaa aaagaagaga
     8461 tacctcttat atttgataga ttttttagaa gtgaaaatgt aagaaataaa gatttagaag
     8521 gttctggaat aggactttca atagctcgca tgattagttt aaacttatct attgatataa
     8581 atgtaacttc tgatgtagat attggaacta catttgaatt gtcaataccc aaaaagttaa
     8641 aataataaaa atataaaaaa ttgtttttag aaatgaagaa tgggtatact aattagtata
     8701 aaatataaaa agaccttata aaagtaaatt tatatttata aatttcatag tataattata
     8761 tagggataat aaagtttaat aggaggattt aaaaatggca aaagttataa atacatctga
     8821 atttagaagt agtgttgaag gtagtaaggg agtagttgta gttgatttct ttgcaacttg
     8881 gtgtggacct tgtaacatgt tgggacctgt atttgcagaa ttaggtgaag agatgaagga
     8941 taaagctaga tttgtaaaag ttgatataga tgagagctta gaaatagctc aacaattcaa
     9001 tgtatctaca gtaccaacta tgattatatt taaagatgga aaaccagtag aaactttaat
     9061 tggattcatg ccaaagaaca aaattgaaat gcaagtaaaa tcatatttat aaaaaatggg
     9121 tgagtattat gagatatgat atagctataa tcggcagtgg tccagctgga ttgtctgctg
     9181 ctataaatgc aaaaataaga aataaaacaa ttatcatgtt tggtaatgat aatttaagta
     9241 ataagcttgt taaagctcct tcaatagata attatttagg tttttatgat ataagtggtg
     9301 atgaattaaa agacaagttt aaatcacata tagatagtat ggatatttca attgaaaata
     9361 aaagaataaa taatatttat gctatgggag aatactttac aataatgtct ggaaatgata
     9421 tgtatgaagc tactactgta atccttgcaa ctggggtaga gtatactaga ccaatcaagg
     9481 gtgaagaaga atttctagga agaggtgtgg gctactgtgc tacatgtgat gcaccacttt
     9541 atagaaataa aaaggtagca gttataggat ataatgaaga atctaaagaa gaagcaaatt
     9601 ttttaagtga actcacttca aagacttatt ttatacctat gtacaaaaaa gataatctta
     9661 tgagaagttc agataaccta gatgattcta ttgaagttat acatgacaga ccagtacaga
     9721 tagatggaga taaacttgta aacaaagtat cttttaaaga aaaccacatt gaagttgatg
     9781 gagtattcgt tataaaagat agtacagctc caagtgcatt ggttcctgga atagagattg
     9841 atggcattca tattaaagtt gacaataata tgaagactag tatagatgga tgctttgctg
     9901 ctggagattg tgttggaaaa ccatattctt atataaaagc agctggtcaa gggcaaatag
     9961 cagctttaaa tgctgtttac tatttagata aattaaagag ggcttaatat ttatattgat
    10021 taacttttta aagatattta taaaaaactg tcttgaaata attttattat ttcaagacag
    10081 ttttttattt ttaagttatt aataaaagta ttttaaaatc tatatgtaat tacttttatt
    10141 aataaaactc aaagttatat tagtaaaaat tggaattatt tataatatac tccaaaattt
    10201 aaaaataatg gttgaaaaat ctaattaaga gtactataat ttaattaagt aattacttaa
    10261 tcaaaacgag gtacttatgg aaaacttggt taaaatattt aaagcattga gtgatgaaac
    10321 tagacttaac atacttatat tagtatccaa gagaaatata tgtcaaaagg ggatttctaa
    10381 atatctaggg atttcagatt ctgctgtttc acaacatata aaaatattaa aagatgttgg
    10441 gataataaca ggatataaag aaggatacta tgttttatat catattaata aagaatcatt
    10501 taatatatgt gtagatttta taaattctat gttaagtaat tctagtgaaa gctttataga
    10561 tgtatttgat gtaaatacaa tacatctagg atgcagtaaa gaatgtaaat ctataaaaaa
    10621 atgttgtaag agaagggaga aataataatg aaagtatgta taccagtaga ggagaataaa
    10681 ggattggata gtaaaccata tggacatttt ggttctgctc caatttttgt tgtgtgtgat
    10741 ttagaaagtg gagaagttaa atctttagat aatggagatt tagatcatga acatggaaaa
    10801 tgtcaaccat taaaagcatt gtcagggaca gctgtagatg cagtagttgt tggaggaata
    10861 ggacaaggag ctataattaa attaaatgga atgggaataa aagtttacag agcagaaggt
    10921 gatactataa gtgctaattt agacttactt aaaaatggaa aattagtaga atttccatca
    10981 gaccatactt gctcacatga cggatgtgga catcattaaa aaaaaacagc tgatgaaata
    11041 tatacttcat cagctatttt ttcttttaat aaatacgatt ttattctttt aaaaccaaaa
    11101 tttagatttt tatatttttt gtagtatcct taataatatt tcaagtttac aagtttacta
    11161 cttttagatt ttttttgtat gtttttctat atagtcaata tcatttaata tgtcattgag
    11221 ttctttatta agtgttttta aatcatcacc atctacattg tagtttaaaa atagttcctc
    11281 tacattatca atgatagaat tgacttttct agtaacttca acaaatgtct taaagttact
    11341 actattttta gcatttagaa atttttttaa atcctttgat ataaatgata ctatttcata
    11401 ttcatcatta aatagacctc taccaatagg atatggttca attctctgta aaaaatattg
    11461 gtctttagca ttaatattat aagtataaga tatttcccaa ccgtctccta aatcaaactt
    11521 gataaaatat aatgtactac ttacttttga tattcttgtg gcaccaagct gtcttaaaag
    11581 aacttcaatc gaatcacctt gaacagtatt aatattcata tatacacatc ccctttagta
    11641 ttattatact atatttttta aaattttata attaaataaa cagtagatat aataatagat
    11701 ataatcataa gtggaaaacc tattttgaaa tactccttaa aagagatagg atgaccgtgt
    11761 ttattaccta tacctgctag tacaacatta gcagatgctc ctattagtgt tccatttcct
    11821 ccaagacaag ctccaagaga tgttgcccac catagtggca ttacatcaat accttcagct
    11881 tgcatagtca aaataagagg aattagtgtt gcaacaaatg gaatattatc taagaaagaa
    11941 gagactatag ctgacaacca taatatcaac aacattgtaa atacaagatg tccttctgta
    12001 agtccaatca atgcttgagc caatttgttt attatcccaa cttctactaa tccaccaact
    12061 actacaaata atcccataaa gaataaaatt gtagaccatt caatacctgc cataatttca
    12121 tctacatctt gttttcctat taaaagcatt atagatgctc ctgtcagtgc tacaacagaa
    12181 gaatctatgt ggattgtact gtgaaacata aaaccaaaaa gtatgaatgc tattaatata
    12241 aggcttttta taagtagagg tttatcttta acagattttt tttcatctag ttttaatatt
    12301 gcatttttag ctctttcatt cacaacaagt tcttttccat aaataaatct aaagcatatt
    12361 atagttatag caagtattac tataacagca ggacctaaat ttattacaaa atccataaaa
    12421 ctaagattgg cagcactacc aatcataata tttggagggt ctcctataag agtagctgtt
    12481 cctcctatat ttgaagctaa aatctgtgtt attaaaaatg gaactggatt aagtcctaat
    12541 atttgtgtaa taactatagt cattggtcca ataagtaaga cggttgtaac attatctaat
    12601 acagctgata aaatagctgt tataatagca aagcaaatca ttatcttcca tgggtcaccc
    12661 ttagctttct ttgcagtcca gatagctata tattcaaata accccgagtt tttgacaaca
    12721 gctacaaata gcatcatacc aactaataca ccaatagtat tgaaatctat atggcttaat
    12781 ccttcatcaa gtgtaagtat attgaatatt atcattaata gtgcaccacc aacagctgct
    12841 acggatctgt ttaccttttc tgtaataata gctgccataa ctattaaaaa tatacatata
    12901 gctattattt gttgaaattc cataatataa gtacctccat ttgtattatt ttgtgcctaa
    12961 tgctagtact atatatagta ttactttttt attgtttttt caatggatgt atgaaagtat
    13021 attatttaac aaagtttttg aaaaattgac agaatttaaa gaaaattttt gttatttaaa
    13081 taaattttaa aattattcca ttgataataa tattaaacga caaacgaaaa aatat
//
LOCUS       DAEMGG010000108        13113 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEMGG010000108 DAEMGG010000000
VERSION     DAEMGG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.109"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            56..244
                     /locus_tag="KPK24_002876"
     CDS             56..244
                     /locus_tag="KPK24_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG2300528.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(298..1167)
                     /locus_tag="KPK24_002877"
     CDS             complement(298..1167)
                     /locus_tag="KPK24_002877"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG2300529.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDVINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(1364..2608)
                     /locus_tag="KPK24_002878"
     CDS             complement(1364..2608)
                     /locus_tag="KPK24_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300530.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYNMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            2899..3210
                     /locus_tag="KPK24_002879"
     CDS             2899..3210
                     /locus_tag="KPK24_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG2300531.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      3303..3395
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3495..4289
                     /gene="thiD"
                     /locus_tag="KPK24_002880"
     CDS             3495..4289
                     /gene="thiD"
                     /locus_tag="KPK24_002880"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG2300532.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            4313..5101
                     /gene="thiM"
                     /locus_tag="KPK24_002881"
     CDS             4313..5101
                     /gene="thiM"
                     /locus_tag="KPK24_002881"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG2300533.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            5124..5756
                     /gene="thiE"
                     /locus_tag="KPK24_002882"
     CDS             5124..5756
                     /gene="thiE"
                     /locus_tag="KPK24_002882"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG2300534.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKQYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            5895..6197
                     /locus_tag="KPK24_002883"
     CDS             5895..6197
                     /locus_tag="KPK24_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2300535.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(6263..7012)
                     /locus_tag="KPK24_002884"
     CDS             complement(6263..7012)
                     /locus_tag="KPK24_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300536.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(7006..7968)
                     /locus_tag="KPK24_002885"
     CDS             complement(7006..7968)
                     /locus_tag="KPK24_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300537.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNALSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDAIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(7972..8430)
                     /locus_tag="KPK24_002886"
     CDS             complement(7972..8430)
                     /locus_tag="KPK24_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2300538.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNFPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            8785..9141
                     /locus_tag="KPK24_002887"
     CDS             8785..9141
                     /locus_tag="KPK24_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2300539.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            9213..9929
                     /locus_tag="KPK24_002888"
     CDS             9213..9929
                     /locus_tag="KPK24_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300540.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            9922..10746
                     /locus_tag="KPK24_002889"
     CDS             9922..10746
                     /locus_tag="KPK24_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300541.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYSLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            10758..11957
                     /locus_tag="KPK24_002890"
     CDS             10758..11957
                     /locus_tag="KPK24_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300542.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIETIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            11966..12778
                     /locus_tag="KPK24_002891"
     CDS             11966..12778
                     /locus_tag="KPK24_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300543.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVKVTGFSSFRNIMGSTESWFMFGIIACICYSFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            12789..>13113
                     /locus_tag="KPK24_002892"
     CDS             12789..>13113
                     /locus_tag="KPK24_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300544.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYK"
BASE COUNT         5010 a         1511 c         1968 g         4624 t
ORIGIN      
        1 catcaatctt tgataaacaa gattttaaat ttgtagcgtc taggaggatt ttattatgaa
       61 agcaaatgtt aatcaagata cttgtatagg ttgtggatta tgtccatcaa tatgtccaga
      121 agtatttgat atgaaggatg atgggaaatc acatgttata gttgatgaag taccaagtga
      181 tgctgaagaa tcagcagcag aagctagaga aagctgtcca gttgatgcaa tagatgtgca
      241 ttaataaaat aaatgcctat aaaaattgaa ttttcaattt ttataggcat ttgtctttta
      301 ttcagttagt atttcaacac ctgtttctgt aactaaaacc atacattccc attgagcaga
      361 taatttacca tcttttgtat aaatagtcca attattttct tcatcaacaa aaatttcgtg
      421 agttccttca tttaccatag gttctattgt gaatatcatc ccaggaacca atagcatctc
      481 agtccctttt ttagatacat aacttacaaa agggtcttca tgaaagctta atccaactcc
      541 atgaccacct atttctctaa caacagagta tccatttttc tttgcataag agttgataac
      601 atcacctata tctcctaaga atccccatgg tttggcaact tcaagacctt tttggacaca
      661 ttcctttgta acttgaacta atttttttgc attttcgtta acattaccta tcataaacat
      721 tcttgaagca tctgaatagt atccgttaaa aatagtagaa acatctacat ttataatatc
      781 tccatctttt aagataatgt ctttacttgg aataccatga caaacttcat tatttactga
      841 tgtacataca cttttaggaa atccattata gtctagtgga gcagggatag ctccattttg
      901 tagtgtaaag tcatgaacca acttatttat atcttctgta gacattccaa tttcgatttt
      961 atctgaaata taatccaaaa tttttgtgtt tattacagca ctttttttta tttcctcaat
     1021 ttgactttca ttttttataa tatcatgagt tggtacaata tgacctaact tagcaaatga
     1081 atctatttta ttatccatat ccatatgaca ttttttatat tttttagtac ttccacacca
     1141 acagctatca tttctattta acttcataac ttttcatcct tcttttatta tttatttaaa
     1201 ttatgggtta cataaactat tttaaaacat aaccattata aatattataa cataaatact
     1261 aagaaatcct ttatagatag tgatttattc aaagtataaa ttattatatg gttattgttg
     1321 atgaaaagag gatagttaaa tgtattattg gatattagcg cacttaatag tttagttcaa
     1381 gtttttcaag aaaatcatat gttagaggac aattttcctt taaagtattt tttgaatctt
     1441 catcaagata ataatgtaca aatgattctg caaaaaattc ttctataaac ttgcttatat
     1501 atgcatgtgc aacttgctta gggtcataaa gcttatatct ttcctgagca aatacttcca
     1561 taaattcttc agaatttgat tttttattta aaacattttt atctatagca tgtgcagttt
     1621 catgtaattc taaatttttt gatgaatgta catttgcata gctagggtta ctataaccta
     1681 ttttagccac tatagaatta tttctatata taccaggtac atctttccaa gtcttgcctg
     1741 atcttaccca acatggaggt aattgatatt ttaaatcact aaattctggt tcatcagtta
     1801 gatttgaatt tattaatttt atttttatat ccttttcttt aagtgtctgt aaatatttaa
     1861 tatttatttt tgcaagtctg tttttcatat tttcaacttc atctttatca taattcaagt
     1921 catcaggaag tataagcatt tgttcaatta tatctatact actgtctttt atattttcag
     1981 attcttcatc aaatttatat tgaagagtat tattttcata gatagttata tttgtattat
     2041 tttctataga ataagagtga atatcctcta aatctaaaat attcatttgt tgtaaaatat
     2101 gttttgtttc gccactccac actactaaag aaagtgaatt aaaattaatt agtggatttt
     2161 cttttgcttt tatatcattg cttatatcta catcaaatac atattcaatt gagtcttcta
     2221 tgttaagact atctgtattc tttttaatat ataaatacgc ttgttttaag tctatataaa
     2281 tctcatctaa agattcgtca taaaattctg ataaattatt agtaaagagt tcttcactta
     2341 aaagttcatt tttatcagtt atactttcaa ttaaaatatt aatttctttt atcatattgt
     2401 aaatacttat ttttctattt gctttttcac tagatacatt gcatggcgca agagcaatgc
     2461 aataaaaatc tttttctttt ttataaaagg ctttaagtga ttttgataaa agtgtaaagt
     2521 ctacacctat ctgtaagttg accttaagtg agtcattttg taataattca gtattatttc
     2581 ttttagctgt atttaaattg ttttccataa tcattgcccc ctttttatta gggtatcata
     2641 atttttttat atgtgtcaat ttgttttttt gaaaattata atttttttga aaattttatt
     2701 ttttataatt tgtgaatttt ttattataga aataaatatg ttattattat gtcaaaattt
     2761 agcattagat tcatgtagag tatgttttat acatatatta taattaattc tttgaagatt
     2821 gcatattcaa aatacacacc atttttttta attctataat ataatattta tatatttaat
     2881 gttaggatgt gatttaaggt gtattttaca tatattataa gatgtaaaga tgattctcta
     2941 tacactggat ataccagtaa tatagttaga agaatgaatg aacataaatt aggaattaat
     3001 tcaaaataca caagagcaaa gggatttgaa aaactagaag tttattttgt gaccaacaca
     3061 aaaagcaatg ctatgaagtt agaatattat ataaaaaaat taactagaaa taaaaaattg
     3121 tctataataa aaaacccaag tatacttata aatttaattg ataataaaga agattatatt
     3181 attggtaagg aaatagaaca attaacataa atatttatag taacattata atgggtgcat
     3241 atggtgtaaa aacttgacaa taaatactat tgtaatataa tgaagttata aatatagtaa
     3301 attaaaatag ctaggggagc caagagctga gaggtatacc gacccttaca cctgatctgg
     3361 ataataccag cgtaggaaag cttagtaaaa attcaagtat gtattatttg tgatatatgc
     3421 tatttctaag ctatacctat tgtaggtata gcttttttaa ttaagttatt taaattaaac
     3481 tttaggaggc aacaatgagt aattataaaa taccaacact tacaatagcc ggctctgatt
     3541 catctggagg agctgggatt caggctgatt tgaaaacgtt tagtgcaatt ggtacttatg
     3601 gtatgagtgt aataacagct ataacagcac agaatactaa aggagttttt gctgtagagg
     3661 atttaaataa gaaaataata aaaaaacaaa tagaggcagt ctttgaagat atacctccaa
     3721 gagctgtaaa aataggaatg gtttcaagtc ctgagattat acttgaaata gttgagaatc
     3781 taaagaaata caatccaaaa tatttggttg tggacccagt aatgatatct aaaagtggat
     3841 attatctatt aaagccagag gcaaaagaaa acttaattaa gtatttgata ccacttgcat
     3901 acataattac accaaatata ccagaggcag aagaaataac aggaataaaa atacataatg
     3961 tagatgatat gaagagagta ggagaagaaa tactacaact tggacctaaa tttgtactta
     4021 tgaaaggggg tcatttagat ggagaagcag tggatatttt agttggtaaa aatatattta
     4081 aagtttataa aagtgaaaga attgataaaa aaaatacaca tggaacaggg tgcacattat
     4141 cttctgcaat aacatcttat ctagcattag gatatgaaat aacagaagca gtaaatctaa
     4201 gtaaaatcta tataacagaa gcaataaaaa gaagttttga tataggtcat ggagtaggac
     4261 cagtacatca tttttataaa tttgagtaat aaaaaaatat tggaggtaga atatgtataa
     4321 tttaattaaa gatgtaaaaa aattaaatcc attggtaatt cattatacta ataatgtaac
     4381 tataaatgat tgtgctaatg taactttagc tgtaggagca agtcctctta tgagtttttc
     4441 atatgaggaa gtagaagaaa tggtaagtgt agcaaactct gtagtaataa atataggaac
     4501 tatgaactca aatatgttgg atttattcct attagctgga aaagcagcaa ataagtacaa
     4561 taaacctgtt gttttggacc cagttggagt atttgcaagc aaagcgagag cagaacttac
     4621 aagtagactt ttaaatgaag tcaaatttag tgttgttaaa gggaatgtat cagaaataaa
     4681 gtttataggt ggctttaatg taaggggtaa aggtgtagat tcatttgatg aagaagaaga
     4741 ttctactgag ataataagaa agattgctga aaagcttgaa tgtgtagttg tagcaactgg
     4801 aaaaatagat ataataacta atggtaaagg tacatataaa ataaacaatg gaactgataa
     4861 attaaaagga attacaggaa ctggatgtat gactgctagc ttgattgcaa gttttatggc
     4921 tgttactgag aatatattag aagcagcaac aatgggtgtt cttacaatgt ctttaagtgg
     4981 ggaattagct aatttaaata atccaccaat aggtacattt aaagaaaatc ttatgaatgc
     5041 tatttatcaa atggatatag atactttaag taaaaattct aatattgaat ttttaaatta
     5101 ataaagagta agggagactt aaaatgattg ataaagaatc tttaaaaaaa tgtttaaagt
     5161 tgtatttagt aactgactct gaaatgctta aaggtagaga cttttataaa tgtcttgaag
     5221 atgcaatatc ttctggaata acaactgtac aacttagaga aaaaaatgct tctggaagag
     5281 agtttttaag aaaagctatg aaacttagag aaataactaa acaatatggt gttaagttta
     5341 taataaatga tagagtggat atagcactta tttgtgatgc agatggagta catgtaggac
     5401 agagtgacat tgatgttaga gaggttagaa agcttatagg aaacaataaa attctaggag
     5461 tatcagcaag aactttagaa gaagctattt gtgcaaaaaa tgatggcgca gactatttag
     5521 gtgtagggtc tatattcaca acatctacta aattagatgc aaaatcagct tcgtttgaaa
     5581 ctgttaaaga aattaaagaa aaagtagaca tgccatttgt actaattgga ggtataaatt
     5641 tagacaatat agataaactt aaatgtttag aaagtgacgg gtatgctata atatcagcta
     5701 tattaaaagc tgaagatatc tctaaagaag tggaaaagtg gacattaaaa atataaaatt
     5761 ggctctggta gtgaaaaaaa tggaaaaatt tgatataatc taagagtaaa atttaagcaa
     5821 ttataaatga taaacttttc tacatatact ttttaatcaa gatatatgta gatttggtta
     5881 aaaaggagct ataaatgatt aggatattgt tagtgtgtgt tggaggcatg tcttctaccc
     5941 tattggtaaa taaaatggag aaggatgcaa aaaaaagaaa tatagactgt aaaatatggg
     6001 cagttggaga aggagatata aaatcggagc ttgataactt tgatatttta cttcttggac
     6061 cacaacttag atttatgctt gatgacgtaa agtctatagt tggagataga gctccagtat
     6121 ctataataga tatggtaaac tatggcactt gtaatggtca tgctgtgtta aattcggttt
     6181 tagaaatatt aaaataaata ataaaagtgg attctatata atagagtcca tttttattat
     6241 ttgttattaa taggcttaaa acttagctta ccatttcttt ttttaatctc cacatagcaa
     6301 gtgaaaaact tgaacagcac aatattaaaa atagtaggat aacaggcagt atatctccaa
     6361 taagaatagt ctcaccaaat attaagctac gtaaagcgtt aataacatgg gtaaatggat
     6421 tgagcatcac tgctactttt aatcctccag ataaactttc tatagggaaa agagctgaac
     6481 ttaaaaagaa aataggaagt acaattgcag tcataattgt ttcataaact atttcatttg
     6541 gcaataataa acttatagaa taagctagac ttgataaaaa gaaggctgtc ataaatatca
     6601 gtactatcat aagtaggatt ccaccaatgc cagattctat ccttactgag aagaatatac
     6661 ttactataca caaaatagtt acttctatga aagaaactaa gatagcttct aataattgac
     6721 caaggacgat tgaataccta ctgataggtg caattaagac tctatagaaa cttccactgt
     6781 tcttcattat aaagtttata ataccaccac tactacaaga agaaaacaca accagtacaa
     6841 tgatacctgg tagtataaaa gcagtataat tattgatatt tatattattc atagtttggt
     6901 ttgcaataga actataaagc acaagccaaa gaaaaggttg taatatagta actacaaatg
     6961 agattggatt tttaaaacgc cacttcatac tacgccataa gatatttaac attcccattc
     7021 ctcctttaag ctttttttag aacttgtaag tgctaaaaat acatcttcga gacttggctc
     7081 tacaatttct attgcatcaa attctatatc atgttctaaa agccatttat ttattaaagt
     7141 aaaagcagtt ctactatcgt ttacacttat aaaaattgaa ttttctcgta cgctcataaa
     7201 ctttactaaa ccagtacttt taattgcatc tttatatttt tttatatctt cagtattatg
     7261 gaaagttata cgtagtatat tttgtctaat gtaactacgt aaacttgatg gtgttccttg
     7321 tgctaaatcc tttccattct tcataataca aatattatca ctcaattgtt cagcttcttc
     7381 aagataatga gtagttagaa atatagttgt gccatactca tctctaattt ttaataacat
     7441 atcccacata gattttcttg aatcgacatc cataccaaca gttggttcat ctaaaaatag
     7501 gattttagga gatgatacca tattcatagc tatgtccaat ctacgtttta caccccctga
     7561 atatgatgaa gtaggatatt ttaagtaact agataagtca aacttatcaa ttaaagagtc
     7621 tattctttgt tttgcaatct gtggttctac tttatacatt ttactttgaa ataccatatt
     7681 ttccataagg gataaatgtt catcaataga gatttgctgt gctacacaag caatttgagt
     7741 acgtatccaa gaaggattat ctactaaatc ttttccaaac attgttacat ttccagatgt
     7801 aggtttgtaa aaggtagtca aaatatttat aagagaagat ttaccagcac cattaggacc
     7861 aagtaaagaa aaaatttctc cagcatttac attaaagctt agagcattta aagctttcac
     7921 accattttta tattctttta ctaaattgtc tatttcaatc gctaacatat attattcctc
     7981 ctctttaagt ggaaatatta tctctgtaat atagttatct gggtttcctt ttatcaacat
     8041 acctggtcct ttaataaata cttctctcca aggagtttgt atttttatat tattttcatg
     8101 aatatattta aatatcattt catacgtttt tggaaaatta ctataagagc ctgtgtgagt
     8161 aaaaaataaa gcttttgttt taggaaattc ttttgtagtt atacccatac gatttggagt
     8221 ttcagcagtt ggaacacata attccatttc tgcaacacca gttttttggt caacatcata
     8281 gtagcaaaag aatggtgcac cgttagactt tccttttata gctttaaata catttggaaa
     8341 atactttgtt gcctcagtca taggtccatt ataacgcatg gtagccactg tgactgattc
     8401 gatatttttg atttcaattt tgcaattcat tgtttacctc ctaatgtgtg ttcatattct
     8461 tactagattt ttaagcatta atacatataa gattttgatt agttgaaaac atataatctt
     8521 atctcgtttg cttgttttca agtctaacat aacataaaat atatatcctc tcatttgatg
     8581 ctccattttg taaagttatt atttaagctt tactttagat tttttagcaa attaaaataa
     8641 agcaatttat aaaataagta tttacaaatt aagtgtatta atcgtataat acacttaata
     8701 ggatactaga taaagtctga aataaaggcg tattttttta aagtaagtgt actatcgtat
     8761 taatacacta gaataggagg aatcatgaat tttgaattag ataatacaac tcccatttat
     8821 ttgcaaatag taaagtatat aaagagacaa atagtaacag gagagttgaa accaggagag
     8881 acaataccat caagaagaga aatggcactt aatctaaaag taaacttaaa tacagttcaa
     8941 agagcatata aggagatggg tgatatgaat ataataaata cttttaaaaa ttatcaaagc
     9001 agtgtaacag ttgatgaaaa tatattaaaa aatttaaaat tagagcttat aaatgaatca
     9061 ctatctgttt ttatagaaga tatgaaagca ataaatgtta gcaaagaaga agttttaaaa
     9121 ataatagaag ataagtatta gttttttaac aaatagaaaa cttagtttag aaagtttatt
     9181 tataagcata ttggatgtgg aggatagtaa atatgataga aataaagaat gtcagcaaaa
     9241 catataaaag gatgcaaggg cttaaaataa gaaagataga agctttaaaa aatgtaagtt
     9301 ttaatataga aaagggaaaa ataactgcat tacttggtat aaatggtgtt ggtaaatcaa
     9361 ctatgttaaa agcaatagca ggacttataa acattgatag tggagaaatt cgtatagatg
     9421 gagaaaaaat aaatgaaaag gtatacaata aattggcttt tgttccagat gtacaatctc
     9481 actttagcaa tacaacaata aaagaaacat ttgaatttat ggaaattttt tattcaaagt
     9541 ggaataaaga aaaatcaaaa gaaatgatgg acatatttaa attggatgaa gatgaaataa
     9601 ttgataactt gtcaaaagga aacattgcaa gagtaaaatt aatacttgga ttttgccaag
     9661 acccagagta catacttctt gatgaaccat ttacaggaat tgatttgttt aagagggaag
     9721 agtttatagg agtaatagcg caatatatgg aggaaaatca agccattatc ataactactc
     9781 atgagatagt agagatagaa agtttagttg atgaggttgt aattcttgat gaagggcaga
     9841 ttataacttc atttaatgca gaagaattaa gagaaagaga aggtaaatct atattagata
     9901 agatgaggga ggtatataag aatgaataaa cttctatatc taataaatgt agatttgaga
     9961 agaagcaata agttttatat agcttatata tcattttttt cactaattac acttagttta
    10021 aatttattcg aaattaataa atttaaaaat tctaaattta ttatgaactt agcaatagaa
    10081 gattttggag gtatatttta tggactaggg atacttagta atcttggttt tatccaaagt
    10141 atattgttct ttgggatatt gggacttttt atatactata tttttatgtg gaaaagagag
    10201 ttcttttcaa ataatcaaag tatttataca cttatgatgt tacctcaaaa taagtttaag
    10261 atttgtatat ctaaatcaat agctttgata acaatgatat atggtttttt gataagtcag
    10321 acagcaacat tatttatatg taaatatata tttaatttca tttttagaaa tatgcctata
    10381 atcaatatga actttgccaa agatttatat tatttaaact tgaagagtat accaattgat
    10441 tttatctctt ttatggctat ctatgtattt ttactactta tagctatttc tattgtaaat
    10501 tgctgtatat tatttatctt ttcctatata aataaatttt atgtaatttt aatcactttg
    10561 ctgattatag caggcttata tttttcaaca atatatataa atataatcac tttatttaga
    10621 aaatattata gtcttatagg atttcaattt aacaatttag tagcagggtt attaagtttt
    10681 ttaattataa tgtttattct aaatggaata tcttatttgc taattaaaaa gaaagtatct
    10741 ctttaaggag gggtggtatg aaattaaaat ctaaaatatc tatacttatt atatttttga
    10801 ctttagcaat atattttagt ataggtttat taaataatga agataattta aagataaaaa
    10861 ctataaaagg tgactctaat gaattagacc atataaacat aaattttgga tatgctttga
    10921 atggatatat aaattcatat tatacaataa atggaagtaa caaaaccaaa aagactgaat
    10981 taataaaact acaaaaggat agtaagaaca caaataattt aattgatgta tctagtggtt
    11041 cagatttaat tgcacatgtt ggatttgaaa tgaatgagga gtcaaaagat ataaactttg
    11101 taatagatga gaaaaaagaa gatttagaaa aaggtgtatt tccatcagaa ggaagtgata
    11161 aagaaattga atatgaaaaa ttagatatga ataattttac aaggacaaga tataaagtag
    11221 ataaaagtac agatgattat tatgcaaata tactagcatc aaaaagatat aaaaaatact
    11281 tatatgtatc tgtactttat gctacaagtg attcatcaga aaataacaaa gaatattcaa
    11341 gtactataga tataataaaa attaatccag aaaataaaaa aatcaagact ataaaaagta
    11401 taaatttaag taattatttt tccgaaaaat accaaaacat agagaccata ggtgtaagtt
    11461 atgctagcaa atatgaggat aagatgtata ttttagtaaa tgtactaaat acaaacatag
    11521 gtgataatag taattctaaa tttcatttac ttgagtataa tcttgaagaa aataaggaaa
    11581 gcataatgga attaaaaact gatagcaaaa tggctttagc aaaagcaaag ttagaagcta
    11641 ataaattata tatggtaatg tcgaatgatt atgataatag ttcaattaca gagttaaatt
    11701 tacttaccta taatatatta gataaaagct tagatgaaga taaatatgtt ttagataata
    11761 gagaaaaaag aggaattaga agtattgaaa ttgattcaaa taaaatcgtt gcatatacat
    11821 caaggctaaa ctctatatta tcttgggata ataaatttat gctatatata ataaataaaa
    11881 attctaagga aattgtatat gaaggagaat tacaatttaa cagttcaaat agtatgttag
    11941 aatttgaaat aaagtaggtg agttgatgaa agatttaata agattattcg aatttgaact
    12001 aaaaagaaat ataaagaatt atatatttat aataatttgt tgttgttctt ttgtaatttt
    12061 aaacatagta aaaaatttaa atgactataa ttacataata gaaaatgcag ttaaaagtga
    12121 acagttaaca aaaatcggaa atacgataga aacggtaaag gttacaggtt tctcaagctt
    12181 tagaaatata atgggttcta cagaatcgtg gtttatgttt ggtataattg catgtatttg
    12241 ctattctttt tttatttggt atagagactt taatggtagg agtaaatcta tatatacact
    12301 tataatgcta cctaaaaata gaatcaatat ttatatatct aaattattaa atatattatt
    12361 tctagtgtat agctatacag tagtacttac aatatctttg tttattgcaa gtaagttgtt
    12421 gcctagacat atgcttggta atgtcacaaa ctatggtttt gtacaagaaa caatatatga
    12481 gttaaaaatg ttacttccat atagctttga aatactattt gtagagtata tatttttatt
    12541 gataggattt gtatctgtag tatttacatc aattcttata aataaatcta tttataaagt
    12601 aagcacttta ataagttttc tatttttagt tatagaaatt ttagtgttta taatttctat
    12661 agaatttatt ataccctacg aatacagtga tgtatttact gttctatatt caagtataaa
    12721 catatttgta tgtagtctta tatctaataa acttttgaag caaaagattg acttttagga
    12781 gggatattat gaaagcaaaa aatataggaa aatatacaat atgttttata atagtatgtt
    12841 ttttaatgat tactatgacc ttaatattga gttattttaa taaggataaa ctatctgtaa
    12901 aaactttaaa aggcaatgta aaagatatag ggagagtaag tgtagtatat acacctaata
    12961 taagcaaatt ttcttatgag gaggtaatta taagtaaaaa tggagtaaga aataatacaa
    13021 acccaaaatc aagaattaga aatgattatt taaatattga caaaaaatac agtgactttg
    13081 ttaatgataa aggaaaagaa tttatatata aag
//
LOCUS       DAEMGG010000109        13107 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEMGG010000109 DAEMGG010000000
VERSION     DAEMGG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.110"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            133..654
                     /locus_tag="KPK24_002893"
     CDS             133..654
                     /locus_tag="KPK24_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG2300545.1"
                     /translation="MDFKIRELINDITQDIIQTYKIQIPIVNINQVVDALGGKVIEDS
                     SLSGYSDGFIRKVDDSFEIVVSPYQPDTRKNFTIAHELGHLFLHMGYGIDDELWNSQD
                     GNQYFRSGNTNKEYQSNEFAAALLMPKHEYKRIMDENTVGNKVDTSKIAEYFNVSSYA
                     ASNRGKWLGYLQW"
     gene            630..2216
                     /locus_tag="KPK24_002894"
     CDS             630..2216
                     /locus_tag="KPK24_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300546.1"
                     /translation="MVRIFTMVDDKEYKTQNVNNVHNSAKNENAFNLKKYKEKLQENI
                     NTDIYEKEKEPNHPEVILFFSFDIANSSLYKNINYSGWAKVLSHIIRKLQYRVYENLK
                     AQLWRVLGDEVIFIIVLKNYDEIYKYIDIIFDILTSTAKDIKSGNIFSTLEGFSESEK
                     YLMKLQNIISLKGAAWIAIVSRNPNFNALENNEQYENISAMYDLSNNYKIFEFLGNDI
                     DAGFRISKQTCPERLVLSFELAYILSRKTDILSKLHIITYKKLKGIWKDKLYPIIWYH
                     NKGKNNDIEFDDSFSFDEIEENELVREYFFNKKGESKLLIDSFMFNSVDKALDKILID
                     RNLSDKIEKIGDVISKTNPSYDKNTIDKDYIKVDLMELHCVAVCYNKSTSKILIAKRS
                     DNRNNNASKWEFGCAKASLETSIINTIKDEYEKDFNINIEPITDCTRKDDCQPIPLAI
                     YQVKKSDGLHKGIITLAEIINDYDISKFEPTSKHNELAWIGEDELEDFNENTVPDFKE
                     TLKLAFKKLNENQLQESTNM"
     gene            complement(2280..2798)
                     /locus_tag="KPK24_002895"
     CDS             complement(2280..2798)
                     /locus_tag="KPK24_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300547.1"
                     /translation="MDKKIDIAKFIVNRIDYYIEKSDNKASFLLLLNSAIIGFLFSGK
                     EKIMNHLSISNIRCLEMLFYIVILCIFVISIYFSIMVLKPRNSKIENEYKSIFYYKEI
                     ASLNNEQYKEAFENAFKDEENLINDALVQIKELSLICDKKMYNVKKSVEAFILGGIIL
                     FIYILVVVFNAI"
     gene            complement(2964..3326)
                     /locus_tag="KPK24_002896"
     CDS             complement(2964..3326)
                     /locus_tag="KPK24_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300548.1"
                     /translation="MEDLCMDALVYERFVRAAFSIKLNNLINRSEDLGGLAEADIFRA
                     ANNLHELNEIKIGTGYAIAIFNNEILESCNVSDNDSNRMIELFDRSLIATSREEILDI
                     IREYETYRGRYLTFNWKR"
     gene            complement(3407..4219)
                     /locus_tag="KPK24_002897"
     CDS             complement(3407..4219)
                     /locus_tag="KPK24_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2300549.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKNEEKSYKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSKPTAVLIESFFCDNKEDYDKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKILANVLSWGYSPSKVLVCD
                     IKDYVPGQTENLYVVGGGACEKISSITKEKFIMIKGNDRFDTLYKALDFINR"
     gene            complement(4219..4650)
                     /locus_tag="KPK24_002898"
     CDS             complement(4219..4650)
                     /locus_tag="KPK24_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG2300550.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGSK"
     gene            complement(4712..4849)
                     /locus_tag="KPK24_002899"
     CDS             complement(4712..4849)
                     /locus_tag="KPK24_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBG2300551.1"
                     /translation="MNWYKIITDFYNNGNWTKEQVKTAVTKNKITASEYKEIVREDYI
                     A"
     gene            complement(4851..5150)
                     /locus_tag="KPK24_002900"
     CDS             complement(4851..5150)
                     /locus_tag="KPK24_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300552.1"
                     /translation="MIYLGNFMETDEQNIKYIGMIHYKPTLLSEEELKNGILIEKLPV
                     QQYVENKEAKLFINIDTKEVFYRYTDIKSSIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            complement(5147..6382)
                     /locus_tag="KPK24_002901"
     CDS             complement(5147..6382)
                     /locus_tag="KPK24_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300553.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENNFTSDNVEGALKEVFQN
                     GVNAKNNVVTALNSKGAEVTTSDTWEEIKNKIDIKEGRLDLRETILANSYSSYLVTNG
                     AIKYIEKYSGSLKALEYEEPYFYAIKGTHLIKINAIDESVIFDITLANANFSCICVTQ
                     EYLFISDNTKLYKINKLTGNEAQSIEGSYYKLCTYGEFIYGIYGDETSSILHKIRISD
                     MYIMLTKDMSSDRIYDFERGKFVCNNNGIYATTEHSNSSGLTECYLTKINFDFSVAKS
                     FRIGGYLYEKNIKFLNDFVFVSDSSRSIEVESGKKSGLAKYDANLNLITYGGSDRYEN
                     FEIYNGYIYTFNSLSSSPFIKINLNTLKEVNSYRTLINTNPQKGMFIINNIIFFIGGG
                     IHRNILSKKVYSDEKGEEL"
     gene            complement(6417..6599)
                     /locus_tag="KPK24_002902"
     CDS             complement(6417..6599)
                     /locus_tag="KPK24_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300554.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGIEPTA"
     gene            complement(6599..6811)
                     /locus_tag="KPK24_002903"
     CDS             complement(6599..6811)
                     /locus_tag="KPK24_002903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300555.1"
                     /translation="MKKQVYYNSLDEKEKIISENSNLYVIEIYETLNENYLVLSSSPI
                     QDEKLSYEELENELLIMTNELQGGLL"
     gene            complement(6821..7459)
                     /locus_tag="KPK24_002904"
     CDS             complement(6821..7459)
                     /locus_tag="KPK24_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300556.1"
                     /translation="MTEKLTENASLGELMAALENVQTDFQTGKNNISSALGSPFIGTD
                     KFGTTKTKIETLKNVLVETINSKNVSATSSETFTNLIEKVNWIFQSIEIFSLKNRIQA
                     TTLNTPSIVYNEVSSIKGTLRFTGELKASKMRADYATAKIEILCGNRKEYFYVTDDTP
                     SASSSFVRFTKDIIVENGMDIKVQILLTSVGAGNLDGSYAARAEIEKLTILR"
     gene            complement(7473..8081)
                     /locus_tag="KPK24_002905"
     CDS             complement(7473..8081)
                     /locus_tag="KPK24_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300557.1"
                     /translation="MLDNDKYLNNKIDTTKTELNTRIDTENEKQNIKIDQLIAGGSNV
                     ASTQIITIDDWVEDAENGFKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIE
                     IRSETRSELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDSISSIQLIDTSIS
                     ITDANDRFTSDKLDGVLEEIMVEISGQRTKGITIVNNLIDMI"
     gene            complement(8186..8566)
                     /locus_tag="KPK24_002906"
     CDS             complement(8186..8566)
                     /locus_tag="KPK24_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2300558.1"
                     /translation="MANAVTTDIARQKMCKARAGDITLPTIVSMAFGDGGIGNDGTII
                     APLSSDIALKNEVFRKDIENYVYPIPTTCRYSTTLLKNEAEGKNINEIGLIDSDGDLI
                     AIKSFGNKYKDSDMEMVFQIDDEF"
     gene            complement(8569..9339)
                     /locus_tag="KPK24_002907"
     CDS             complement(8569..9339)
                     /locus_tag="KPK24_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG2300559.1"
                     /translation="MITSKKGKEMLLTLSPIYEQSLVMNSIYEAIGSEFDNLELLNKE
                     IELQLFPQTATWGLEFWENRVGLSTNIDEDIEARRRKVIAKLQMKYIVNPNRLATIIK
                     SYTGTDVYIKENIAPYTFKVTANVDDVINYEDFKYITNKTKPSHLHWMPSFALKFTDI
                     EKFEVKMINRIFIDFRGNISNFLDGMWLLNGSKNLSAYILYNEPISLNMKNKLFVKES
                     EVFTNLKVIIKKNLYYLNGLEMLNGNKLLNAELREEVL"
     gene            complement(9336..10469)
                     /locus_tag="KPK24_002908"
     CDS             complement(9336..10469)
                     /locus_tag="KPK24_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG2300560.1"
                     /translation="MERELPIPVFLTEDEDSVHERMLSNFQDVSTLEGDFIYDATRPT
                     AEQIAELKQLGLQNNLKIAFPQTSYGTYLEWLGECKGVFKNQPTKATGVITFTGVQGT
                     IITKGTIVTTIATDEKQSIEFELLETKTIGENETVDIKAESRIVGTIGNVSKGSISVL
                     LGSINGVKSVTNKDFKGGTDIEDEEHFRERVLVAEQEDKLSGASSDYIRWAKEVDGVG
                     YAYVVPEWNGAGTVKVLILDKNRKAATQELIDKVQEYIYPLNISEGENRDGKAPIGAL
                     VTVVTPDTLLINVKASFIFSNSFNEETVLNNLKTKIDKYLDKIDLGGTVSYNAIQAIV
                     GSMMLTDEGIQDFSNLTINDAKENIKLQDQVVGIGEIVNEVVG"
     gene            complement(10478..10936)
                     /locus_tag="KPK24_002909"
     CDS             complement(10478..10936)
                     /locus_tag="KPK24_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG2300561.1"
                     /translation="MPNLFPQSETFETVELKNNDENELDLKGSFLFDFEKGEFVKNAD
                     GTLKKCDKVQAYKQWCQKAILTPRYKKAAYTNIYGSEIKDLIASNLSQSAKELEITRL
                     IKETILVHPYTKEVGEFSFNWLENSRLVEYEFDVLTIDDENIVIDGNIKR"
     gene            complement(10911..11276)
                     /locus_tag="KPK24_002910"
     CDS             complement(10911..11276)
                     /locus_tag="KPK24_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300562.1"
                     /translation="MTDARFNGIARILKENMNKSVANGTFGMGCELAEITANGLKVNG
                     YKDEIQDYLVLENLTLKEDYFTFSDEALSGEYRHKHKIETPKELKPLRIGDNVLVAVM
                     GAEFVVIGRVVNAKPISSK"
     gene            complement(11269..12231)
                     /locus_tag="KPK24_002911"
     CDS             complement(11269..12231)
                     /locus_tag="KPK24_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HBG2300563.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLVEPKQLKDI
                     NIKKGDKIILIDIAYESKKEETIFDGVVWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     QFKENTATQRIEYYCKQWNIPYYNLANTGKKLAKVIHKTNILDMIKKDLKETATKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDADKFGTLQKIKQDEKIKNAKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKSKEYYVIDVTHTLDSRPKMKLNIGSLEYIRRKFYNND"
     gene            complement(12228..12899)
                     /locus_tag="KPK24_002912"
     CDS             complement(12228..12899)
                     /locus_tag="KPK24_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG2300564.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVNPQDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPFQYDASYCRYSELKNPIEVVAMLEKWVDQAEPLRLIIT
                     GFGYNGLVTISSFSNTQTAGREEDRDIEITFRTYRELKIETLKKETKSNAKTDLKDNR
                     PNNKTTPKIYTVKASDTLYKIAKNLLGKGSRWPEIYNIPENKKVIGKNPNIIKKGQKL
                     VIPSK"
BASE COUNT         4250 a         2209 c         1406 g         5242 t
ORIGIN      
        1 aattatattg tataatacaa aaggttaatg aaaataatta atattaaatg taccaaaaaa
       61 aatagttttt gatataatga atattataat aaataattat ttaaataaaa tattatataa
      121 ggatgtgatt ttatggattt caaaatcaga gagctaatta atgatataac ccaagatatt
      181 atccaaacat acaaaatcca aattccaata gtaaatataa atcaagttgt tgatgcttta
      241 ggaggcaagg taatagaaga tagttcttta agtggatact ctgatggatt tattagaaaa
      301 gttgatgatt catttgaaat agtggtatct ccttatcaac cagataccag aaagaatttt
      361 accattgctc atgaacttgg acatttattt ttacacatgg gttatggcat tgatgatgaa
      421 ctatggaata gtcaagatgg aaatcagtat tttagaagtg gcaataccaa taaggagtat
      481 caatccaatg aatttgcagc agccttgttg atgcctaaac atgaatataa aagaattatg
      541 gatgaaaaca cagtaggtaa taaggtcgac acttcaaaaa ttgcagaata ctttaatgtt
      601 tcctcttatg cagcatctaa tagagggaaa tggttaggat atttacaatg gtagatgata
      661 aagaatataa aactcaaaat gtaaataatg ttcataattc agctaaaaac gaaaacgctt
      721 ttaatcttaa aaagtataag gaaaaacttc aagaaaatat taatactgat atttatgaaa
      781 aagaaaaaga acctaatcat ccagaagtga ttttgttctt ttcttttgat atagctaatt
      841 catcattata taaaaatata aattatagcg gatgggctaa ggtattatca catataattc
      901 gtaagttaca atatagagtt tatgaaaacc ttaaagcaca actttggaga gttcttggtg
      961 atgaggtaat tttcattatc gtactaaaaa attatgatga aatttataaa tacatagata
     1021 taatctttga tattttaaca agcactgcta aagatataaa aagtggtaat atattctcta
     1081 cactagaagg attttctgaa tctgaaaaat acttaatgaa acttcaaaat attatttcat
     1141 taaaaggagc ggcctggatt gctatagtat caagaaatcc taattttaat gctttagaaa
     1201 ataatgaaca atatgaaaat atttctgcta tgtacgattt atccaataat tataaaatat
     1261 ttgagttctt aggaaatgat attgatgctg gtttcagaat atcaaagcaa acatgcccag
     1321 aaagacttgt tcttagtttt gaactagctt atatattatc aagaaaaact gatattttat
     1381 ctaaattaca tataattaca tataaaaaat taaagggtat ctggaaagat aaactatacc
     1441 ctatcatttg gtatcataat aaaggaaaaa ataatgatat agaatttgat gatagttttt
     1501 cttttgatga aatagaagaa aatgaattgg ttcgagaata tttttttaat aaaaaaggag
     1561 aaagtaaatt actaattgat tcttttatgt ttaattctgt agacaaggct ttggataaaa
     1621 tacttataga cagaaacctt agtgataaga ttgaaaaaat aggtgacgta atttctaaaa
     1681 caaaccccag ttatgataaa aatacaatag ataaagacta tataaaagta gatttaatgg
     1741 aattacactg tgttgcggtt tgttataata aatcaacttc aaaaatatta attgcgaaaa
     1801 gaagtgataa tagaaataat aatgcgagta aatgggaatt tggttgtgct aaggcaagtc
     1861 tagaaacttc aattataaat actattaaag atgaatatga aaaagatttt aatataaaca
     1921 ttgaacctat tactgattgc acaagaaaag atgattgcca acctatacct ctagcaattt
     1981 atcaagttaa aaaaagtgat ggtttacaca aaggcattat aactctcgca gaaataataa
     2041 atgattatga tatttctaaa tttgaaccca catcaaaaca taatgaactt gcatggatag
     2101 gagaggatga acttgaagat tttaatgaaa acacagtacc tgattttaaa gaaactttaa
     2161 aactagcatt taaaaaatta aatgaaaatc aattacaaga atctacaaat atgtaaatag
     2221 tattcttcta tgatttgatt ctatcttcat agaggaatac taaccaaata aaacattttc
     2281 taaatagcat taaaaactac tacaagtata tatataaaca atataatacc acctaaaata
     2341 aaagcttcta ctgacttttt tacattatac attttcttat cacagattaa agataattct
     2401 tttatttgaa caagtgcatc attaatcaag ttttcttcgt ctttaaatgc attttcaaat
     2461 gcttctttgt actgttcatt atttaaagat gctatctctt tataataaaa aatagattta
     2521 tattcatttt ctattttaga attccttggc tttaaaacca taatcgaaaa ataaattgaa
     2581 ataacaaata tacataatat aacaatgtaa aatagcatct ctaaacatct gatattactt
     2641 atacttaagt gattcattat tttttccttc cctgaaaata aaaaacctat aattgcgcta
     2701 tttaataata gtaaaaaact agctttatta tctgattttt caatatagta atcaattcta
     2761 tttactataa acttcgctat gtctattttt ttatccatga ttttactacc tcatttcatt
     2821 aaattttata ttgattcaaa attttatttt ctacttatta tcattgaata aattattatt
     2881 tggaaataat cttattgaaa taataaaaat atgtgaaaga aagtaataac taactataat
     2941 tactacttcc taacttaaac attctatctc ttccaattaa aagtaagata tcgtcctcta
     3001 taagtttcat attctcttat aatatccaat atttcctccc tagaagttgc aattaaactt
     3061 ctatcaaata gttcaatcat tctattgcta tcattatcag aaacattaca agactctaat
     3121 atctcattat taaatattgc aatagcatac ccagtaccta tttttatctc atttaattca
     3181 tgtaaattat ttgctgctct aaagatatca gcttcagcca atccacctaa atcttcgctt
     3241 ctattaatca agttattaag tttaatacta aaagctgctc ttacaaatct ctcataaacc
     3301 aatgcatcca tacataaatc ctccattatc acaataatat atttacatta tattacaata
     3361 atagagttat cacaacaaac aaccacttct agttgataac ttttatctat ctattaataa
     3421 aatccaatgc tttataaagt gtatcaaatc tatcattacc ttttatcata ataaattttt
     3481 ctttagtaat agaacttatc ttttcacatg cgccacctcc tacaacatat aaattttccg
     3541 tctgacctgg tacgtaatct tttatatcac aaactaaaac tttgcttgga ctatagcccc
     3601 aactaagcac attcgcaagt atcttatcaa cttctccatc ataaacaatt gtatgtttgt
     3661 acatctgttt aactccctca ttatttatat ttttatttaa tacaccttct acaattaact
     3721 tagcaatacc ttcatgacct agtttcttag ctttatcata atcttcttta ttatcacaga
     3781 agaaactttc aattaatact gctgtaggct ttgaactatt taagatatat aatcttttat
     3841 ctaatttagc acctctattt ttaaatactg tacctagttt atcacatatt ctagttgcat
     3901 actctaagcc tttattacta taatatagga cttctgaacc tttaccttga ccgttacttg
     3961 catttaaatg taactctata agtaaatcat atcctccact attaactcta ggtattttat
     4021 aagacttttc ttcattctta gttttaaact gcttttctgg gcatattatt acatctacct
     4081 tatgcccttc ttttctaaat gtatctgcta atactggtgc aagagatttg ttgtattgat
     4141 actcgttaac tactccatca gcagaagtac atgctccact ttttaaaata ctgtgtccta
     4201 ctgttataca tattttcatt atttactacc tcctttaaca tttaattcat ctgtcatagt
     4261 atctaataaa ctacctattt tctcttttaa tcgtttagga actggtaatc cacataagta
     4321 catatttttt aatatactta cactttcata tagaataaat aaaatagaga aaaattcaga
     4381 tattccaaga tgatttaatc gcaaaaaatc aacccaatct tgtggcaaca taaataaaaa
     4441 gttaaacttt gtaagaatgt caactactgc tagaaaaaat atacatgcta tcattgctac
     4501 ttttcttatt cctccattta ttccaaaaga gctattaaac tgatgtgttt ttattgctct
     4561 taagcaacct aacagtgtat caaatgctat tgctaatatt actaatttta taaatatatt
     4621 tgttgctaaa aaaactattg ttacgttcat atttcctcct tattctgcat taaaataaga
     4681 cttagaaatt atctaagtct atttaaaaga actacgctat atagtcctct cttacaattt
     4741 ctttatattc acttgctgtt atcttattct ttgtaaccgc tgttttaact tgctctttag
     4801 tccaattacc attattatag aaatctgtta ttatcttata ccaattcata ctatatcacc
     4861 ccattactca ttaattgaaa tgttaaatct gctattgttt gttctgtaga atttacttta
     4921 tcttctatgc tacttttaat atctgtatat ctatagaaaa cctctttagt atctatattt
     4981 ataaataact ttgcttcttt attttctaca tattgttgta ctggcaattt ctcaattaaa
     5041 attccatttt ttaactcttc ttctgatagt aaagttggtt tatagtgtat catcccaata
     5101 tattttatat tttgttcatc tgtctccata aaatttccta aataaatcat aattcctctc
     5161 ctttttcatc tgaataaacc ttttttgata atatatttct gtgaatgcca ccaccaataa
     5221 aaaaaattat attgtttatt ataaacatac ctttttgagg atttgtgtta atgagcgttc
     5281 ggtagctatt aacttcttta agagtattta aatttatttt tataaatggg ctactagaaa
     5341 gtgaattaaa tgtatatata tatccattat atatttcaaa attttcatat ctatctgacc
     5401 caccataagt aatcaaattt agatttgcat catattttgc taatccactt tttttaccac
     5461 tctcaacttc tatgcttctg ctactatcag atacaaaaac aaaatcattt aaaaacttaa
     5521 tgtttttttc atataaatat cctccaattc taaaactttt cgcaacacta aaatcaaaat
     5581 ttattttagt taggtaacat tctgtaagac cacttgaatt tgagtgttct gttgtagcat
     5641 aaataccatt attattacat acaaatttgc ctctttcaaa gtcataaatt ctatcagagg
     5701 acatatcttt agttagcatt atatacatat ccgatattct tattttgtga agtattgaag
     5761 aagtctcatc tccatatatt ccataaataa attccccata agtacatagc ttataataag
     5821 aaccttctat tgactgtgct tcatttcctg ttaacttatt tattttatat aatttagtat
     5881 tgtcagatat aaataaatac tcttgagtaa cacagataca tgagaagtta gcattggcta
     5941 aagtaatatc aaaaattaca gattcatcaa ttgcattaat tttaattaaa tgtgtacctt
     6001 taattgcata aaaatagggt tcttcatatt ctaatgcttt taaactccca ctatattttt
     6061 caatatattt tatagcacca tttgtaacca aatatgaaga ataactattt gcaagtattg
     6121 tttctcttaa atctaatctc ccctctttta tatcaatttt attctttatt tcttcccatg
     6181 tatcgcttgt agtaacctct gctcccttgg agtttaatgc tgttactaca ttatttttag
     6241 cattaactcc attttgaaaa acctctttta atgctccttc tacattatca cttgtaaagt
     6301 tattctctgt atcttctata gttacattct ttgcttctaa tacaagattt ctaactttat
     6361 taactaactc tttaaaagtc atttagtcac cttctttcaa taaaaaaaag aacctactac
     6421 gctgttggtt ctattccttc tactactcca ctatttttta taatataatc ctctactgct
     6481 tttctgtatt ctgtgttagt tacatcatca agttgaaact ctctattttt caaagggttt
     6541 aaccctccat ttaatattct ttctgctaat attcttacca caacattatt tatattcatt
     6601 ataacaatcc tccttgtaat tcattagtca taattaataa ttcattttct aattcttcat
     6661 aactaagttt ttcatcttgt attggactac ttgataacac taaataattt tcatttaaag
     6721 tttcatatat ttctataaca tataaattag agttttcact aattattttt tctttttcat
     6781 ctaaagaatt ataatatact tgttttttca taatttattc ctaccttaat attgttaatt
     6841 tttctatttc tgcacgagct gcatatgaac catctaaatt tcctgctcct acagatgtta
     6901 ataatatttg tacttttata tccatgccat tctcaacaat aatatctttt gtaaatctta
     6961 caaaagaaga agatgcacta ggagtatcgt cagtaacata aaagtattct tttcggtttc
     7021 cacatagtat ctctattttt gctgttgcat aatcagcacg cattttagac gctttaagtt
     7081 cgcctgtgaa tcttaatgtg ccttttatac tagatacttc attgtaaacg atgctaggag
     7141 tgtttaaagt tgtagcttga attctatttt ttaaagaaaa aatttctatt gattgaaaaa
     7201 tccagttaac tttttcaatc aaattagtaa atgtttcaga tgatgttgct gaaacatttt
     7261 tagaattaat cgtctccact aatacatttt ttaatgtttc tatttttgtt ttagttgtac
     7321 caaatttatc tgttccaata aaaggactac ccaatgcact agatatatta tttttaccag
     7381 tttgaaaatc agtttgtaca ttttctaatg ctgccataag ttcccctaaa ctagcatttt
     7441 cagttaattt ttctgtcata ttttcacctc gcttatatca tatctattaa attattcact
     7501 atagttattc ctttagttct ttgaccactt atttctacca ttatttcctc taatactcca
     7561 tctaacttat cacttgtaaa tctatcatta gcatctgtaa tacttatact ggtatctata
     7621 agctgtatac tgctaataga atcctctatt ttcttagatg aataagtagt catttcagac
     7681 actctgttat catctacagt tgcattaata aaatgagttt ctgcatttcc atttatcaca
     7741 taaacgttta attctgacct tgtttcactt ctaatctcaa tagaattatc atctataatt
     7801 ttaaagtttg taactacatt ttcttttgta gtagcatcta taatatttac aactattctc
     7861 tgtgttaaca aactatgtgt tacagttgct ttgaatccat tttctgcatc ctcaacccaa
     7921 tcatcaattg ttattatttg agtagatgcc acatttgaac cacctgcgat taattggtca
     7981 attttaatat tttgtttctc attttctgtg tcaattctag tatttaactc tgttttagtt
     8041 gtatcaattt tattatttaa atatttatca ttgtctaaca atttttgttg tcgattatta
     8101 aactcatttg catgagctgg tgtagttatt aaatattctt caatctcatt attaaaatct
     8161 aattcattag gcatttattc acctcctaga actcgtcatc tatctgaaaa accatttcca
     8221 tatcactgtc tttatactta tttccaaaag atttaattgc aattaaatcg ccatcagaat
     8281 ctattaaacc tatttcattt atgttttttc cttctgcttc attttttaat agagtggttg
     8341 aatatctgca agtagttggg attggatata cataattttc tatatctttt ctaaacacct
     8401 cattttttaa cgctatatca cttgaaagtg gagctattat agttccatca ttcccaattc
     8461 ctccatctcc aaatgccatg ctaactatag taggtaatgt tatatcaccc gctcgagctt
     8521 tacacatttt ttgtcttgca atgtctgttg ttactgcatt tgccatactt ataacacttc
     8581 ctctcttaat tctgcattga gcagtttatt tccatttaac atttctagcc catttaaata
     8641 atataaattc tttttaatga ttactttaag atttgtaaat acttcacttt cttttacaaa
     8701 taacttattt ttcatgttta agcttattgg ctcattataa agtatataag cacttaagtt
     8761 tttactacca tttaataacc acatgccatc taagaaatta cttatattcc ctctaaaatc
     8821 tataaatatt cgattaatca tctttacctc aaatttttct atatctgtaa atttgagtgc
     8881 aaaagaaggc atccaatgaa gatgagaggg tttcgtttta tttgttatat atttgaaatc
     8941 ttcataatta ataacatcat caacattagc agttacttta aaagtgtatg gagctatatt
     9001 ttcttttata tatacatctg taccagtata gctttttatt atagttgcta gtctgttagg
     9061 attaacaata tatttcattt gaagcttagc aatgactttt cttcttctag cttctatatc
     9121 ttcatctata ttagtagata aacctaccct attttcccaa aattcaagtc cccatgtagc
     9181 agtttgagga aataattgta actctatttc tttatttaat aattctagat tatcaaattc
     9241 gcttcctata gcttcatata tagagttcat aactaaagat tgttcataga taggagataa
     9301 tgttagaagc atttctttac cttttttaga agttatcatc caaccacctc gttaactatt
     9361 tcccctattc cgaccacttg gtcttgcaat tttatatttt cttttgcatc atttatagta
     9421 agattagaaa agtcttgtat accttcatct gtcaacatca tagaacctac tatcgcttgt
     9481 atagcattgt atgaaactgt tccccctaaa tcaatcttat ctaaatattt atctatctta
     9541 gtttttagat tgtttaatac tgtttcttca ttaaaactgt tactaaatat aaaactagct
     9601 tttacattaa taagtaatgt gtcaggtgtt acaactgtaa ctaatgcacc gataggagct
     9661 ttcccatctc tattttctcc ttctgatata tttaatggat atatatattc ttgaaccttg
     9721 tctattaact cttgtgttgc tgcttttcta tttttatcta gtattaatac ttttactgtc
     9781 cccgctccat tccattcggg aactacataa gcatatccaa ctccatctac ttctttagcc
     9841 catcttatat aatctgaact agctccacta agtttgtctt cttgctctgc tacaaggact
     9901 ctttctctaa aatgttcttc atcttctata tctgttccac ctttgaaatc tttattagta
     9961 actgatttaa caccattaat agaacctagt aaaacggata tactaccttt agacacattc
    10021 cctatagttc ctacaatcct actttctgct ttaatatcta ctgtttcatt ttctcctata
    10081 gttttggttt caagaagctc aaattctatg ctctgttttt catcagttgc aatagtagtt
    10141 actatagttc cttttgtaat gatagttcct tgtacacctg taaatgtaat aactccagta
    10201 gccttagttg gttgattttt aaatactcct ttacattcac caagccactc taaataagtt
    10261 ccataagaag tctgaggaaa tgctatcttt aaattatttt gtaatcctag ttgttttaat
    10321 tcagctatct gctctgctgt aggtcttgtt gcatcataga tgaagtcacc ttctaatgta
    10381 gaaacatctt gaaagttgct taacatcctt tcatgtacag agtcctcatc ttctgttaaa
    10441 aatactggta taggtagctc tctttccata taatcaccta cctttttata ttgccatcaa
    10501 ttactatatt ttcatcatct attgttagta catcaaattc atactctact aacctgctat
    10561 tctccaacca attaaagcta aactctccta cttcttttgt gtaaggatga accaaaatag
    10621 tttcttttat taatctagtt atttcaagct cttttgcact ttgagataag ttactagcta
    10681 ttaagtcttt tatttcactt ccataaatgt ttgtataagc tgcttttttg tatctaggtg
    10741 ttaatatagc cttttgacac cattgtttgt acgcctgcac cttatcacat ttttttagtg
    10801 ttccatctgc gtttttaaca aattcacctt tttcaaaatc aaataaaaaa gaacccttta
    10861 ggtccaattc attttcatca ttatttttta attctacagt ttcaaaagtt tcactttgag
    10921 gaaataggtt tggcatttac aaccctccca attactacaa attcagctcc cataacagct
    10981 actagcacat tatcgcctat acgtagtggc ttcaattcct ttggagtttc tattttatgc
    11041 ttatgtctat attctccact taaagcttca tctgaaaaag taaaataatc ttcttttaat
    11101 gttaaattct ctaatactag atagtcctgt atttcatctt tatagccatt tacttttaat
    11161 ccatttgctg ttatttctgc aagttcacaa cccattccaa aagtgccatt tgctacactt
    11221 ttattcatat tttctttcaa tattctagca attccattaa atctagcatc agtcattatt
    11281 ataaaatttc ctccttatat attctaaaga ccctatattc agcttcattt tcggtctaga
    11341 atctagtgta tgagtgacat ctataacata atattcttta ctttttaaac ttactttgtc
    11401 acctgctctt attctattta tatctactgc acaatctaca cttattgttt cctctccact
    11461 attgaacatt gcttctgctg ctttcttagc ttctttagca ttttttatct tttcatcttg
    11521 tttaatcttt tgtagtgttc caaacttatc agcatctttt ttatatgtcc caattatagg
    11581 cgcttttgta ttttcgtctt tactcttacc taaaactttt acacttgtta ctgcatcatt
    11641 aaaactactt gtaaagttag catcttctaa tatactatct aatttatata catttgcatt
    11701 agtaccaagc ttgaataatt ttaatttatt atccatcctt actctaaata agtctccacc
    11761 ttttgttgct gtttctttta agtccttttt tatcatatct agtatatttg tcttatgtat
    11821 tactttagca agtttcttcc ctgtattagc taagttgtaa tagggtatat tccattgctt
    11881 acagtaatat tcaattctct gtgttgctgt attttcttta aactgatatt gttcctctga
    11941 ttcttccatg taaactgttc tttctctgca agacaatgtt agtttcttac tcttttcact
    12001 ccttctagtt tcccatacaa ctccatcaaa tattgtctct tcttttttac tctcatatgc
    12061 tatatcaatt agaattattt tatcaccttt tttaatattt atatctttaa gttgtttagg
    12121 ttctactaat gatacatcca tcttatatgc aactccgtct atagcttctg aaagagttat
    12181 tccttcattg aaatttgcaa tatcatattt cccgtttaat attattttca tttactaggt
    12241 atcaccaact tttgaccttt tttaattata ttaggatttt taccaatgac ttttttgttt
    12301 tcgggtatat tataaatctc tggccacctt gaacccttac ctaaaagatt tttagctatt
    12361 ttatataatg tatcacttgc tttaacagta tatattttgg gagtggtttt attgttaggt
    12421 ctattgtctt ttaaatctgt tttagcatta cttttagttt ctttttttaa tgtctctatc
    12481 ttcagttctc tgtaagttct aaatgttatc tcaatgtctc tatcttcttc tcttcctgct
    12541 gtttgagtat tgctaaaact agatattgta actaacccat tgtagccaaa accagttata
    12601 ataagtctta aaggttcggc ttggtctacc catttttcaa gcattgccac tacttcgatt
    12661 ggatttttta actcactgta tctgcaataa gaagcgtcat attgaaaagg tagaaatgtt
    12721 ttaaatgata tttctcttat cttctcccct tcttttttaa tgtcaaattc acctaaattc
    12781 actatgtcaa cagtttcaaa ccttttttct tttttaatag atagagaatc ttgtgggttt
    12841 actggaaaat ggaaatctat tttttctttt tcatttttta gataaatatc tattaccaag
    12901 ttatcacttc ctttctaaag tattttttaa cattacaccg cagttttctt accataaatt
    12961 cacttccttt gattttttgt ataaaaaaac acctactcat tcgtaagtgc tttcctctat
    13021 tttatttaaa caatgaaatt tgagtaaaaa aatatctact catttataga tattttataa
    13081 attaatagtt ttataataaa attttta
//
LOCUS       DAEMGG010000110        12934 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEMGG010000110 DAEMGG010000000
VERSION     DAEMGG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.111"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(93..995)
                     /locus_tag="KPK24_002913"
     CDS             complement(93..995)
                     /locus_tag="KPK24_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2300565.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(1045..1506)
                     /locus_tag="KPK24_002914"
     CDS             complement(1045..1506)
                     /locus_tag="KPK24_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG2300566.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(1558..2013)
                     /gene="def"
                     /locus_tag="KPK24_002915"
     CDS             complement(1558..2013)
                     /gene="def"
                     /locus_tag="KPK24_002915"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG2300567.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(1985..2248)
                     /locus_tag="KPK24_002916"
     CDS             complement(1985..2248)
                     /locus_tag="KPK24_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2300568.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(2345..3061)
                     /locus_tag="KPK24_002917"
     CDS             complement(2345..3061)
                     /locus_tag="KPK24_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300569.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(3058..3741)
                     /locus_tag="KPK24_002918"
     CDS             complement(3058..3741)
                     /locus_tag="KPK24_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300570.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(3738..4583)
                     /locus_tag="KPK24_002919"
     CDS             complement(3738..4583)
                     /locus_tag="KPK24_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300571.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(4697..5287)
                     /locus_tag="KPK24_002920"
     CDS             complement(4697..5287)
                     /locus_tag="KPK24_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2300572.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEYFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            5576..5809
                     /locus_tag="KPK24_002921"
     CDS             5576..5809
                     /locus_tag="KPK24_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG2300573.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            complement(6040..8427)
                     /locus_tag="KPK24_002922"
     CDS             complement(6040..8427)
                     /locus_tag="KPK24_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG2300574.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGCTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(8619..9374)
                     /locus_tag="KPK24_002923"
     CDS             complement(8619..9374)
                     /locus_tag="KPK24_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG2300575.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            complement(9399..10550)
                     /locus_tag="KPK24_002924"
     CDS             complement(9399..10550)
                     /locus_tag="KPK24_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG2300576.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            complement(10766..11506)
                     /locus_tag="KPK24_002925"
     CDS             complement(10766..11506)
                     /locus_tag="KPK24_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG2300577.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            complement(11578..12567)
                     /locus_tag="KPK24_002926"
     CDS             complement(11578..12567)
                     /locus_tag="KPK24_002926"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG2300578.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
BASE COUNT         4271 a         2201 c         1279 g         5183 t
ORIGIN      
        1 attaattata tatttaaatt ttatatttgt caataaattt taattttaat aaattttaat
       61 catcatagta aaaaaaattt ttttatataa aactaatcaa aaaaagatat ctgtacatct
      121 cctgagtttt ttaacttttc cttcattatt ttagagtaac tgcttgatag agaatactta
      181 tatctaagtt cattaaccat tttatacaat tgatttttat actccctatt aagtgaacct
      241 gcactatata gtattgataa tttatcaaat aattcaggaa actctttttt aacaaagtca
      301 aaaaaaacac ctctagttat accccttaag tataaagtcc caggtaaaac ataatgtaca
      361 ttacattctt ttgcatgtct aaatagcgaa tttatattat caaaatcatc tgtaatatat
      421 ggtataattg gcataatatg tagaccaact gatgcgttag tttttctaaa ttcttttaac
      481 attttaaatc gtctcattga atccacacca tttggctcaa taagcctttg tgtatcttca
      541 tttactgtgg taattgtaga tgctacattg atatatgtaa tcctagatag cttatcaatt
      601 aaatcgtaat ctcttaaaat taaatctgac tttgtagaaa taatagctgg tgtcttgtat
      661 tttattagca ggtttaatat ttctggcata attttatagt cagcttctat tggttgataa
      721 ctatctgtaa ctcctcctat attgattact tctttcttcc atttactgct tttaagttgt
      781 ctctcaagtt tttctactat atttgttttt acataaatct cttcaaaata attattggag
      841 ttgatgtact tatgagagta aatagcataa caatatttac atccatgctc acatccacga
      901 taaatattta aatcccattt atatggcata ctccttttta atttattaca agctatttca
      961 caatttattg gaatatattt tattttatta tacaaataat cacctcttta tatacaataa
     1021 tactataatg ttaatctata gtaattaaaa catgatattt ccagaccaca attcataatc
     1081 tttactatat ttacaaagta atatattcca ttcatttatt ggtattcctc cacatgttgc
     1141 tttttcttct aaattatata agcattgctt taaatacatt ttattatgcc aatcatcata
     1201 taaattttct aaatttaaac tatgttcatt agatatatca tttgcaaaat ttctattttt
     1261 ccattttaat atacttgtat atttaagttc attaggttta taatttcttt ttaacatttc
     1321 atcatgtaca atttttgtat aaaatttaaa ttcatctata ctataattca atactttatt
     1381 cactaataga tggtttggag tacctttttt atctatagaa ccttttatag caagtagctc
     1441 tctccattga cttactaatt gatttttagg taatacatca attaaatcct tatgccataa
     1501 tctcataatt taactcctta ctataaaata ttttataaat tcaataacca atctatctta
     1561 caattttaca tgttcaataa ttttatctgt aaaaactata ccatttaaat ggtctatctc
     1621 atgacaaaga cattttgcca taaaacctga acctttaatt tctattttct caccgtattc
     1681 gtttagagct tgcactgtaa ctttttttgg tctttttaat ttaccccata cttcaggaaa
     1741 acttaaacat ccttcaacaa caatttgttc cccttcttgt tcaataattt caggattaac
     1801 aagcttaata agtccttctc ctaaatctat aacaactaat cgctttaata ctcctacttg
     1861 gcatgcagct aatcctcctc catttggagt attgtacatg gtctctgcca tgtcatttaa
     1921 tatttctctt atcttattat ctacgttttc tacatactta ctcttttttc ttaaaatttc
     1981 atcatcaaat gttcttatct ttcttatagc cataatattc tcccctactt atctgtgctc
     2041 ttgattttct ttcactttct tcaaataaaa atacactttc aattggttca ttaaaaattc
     2101 ttgaaatatc atatgccaac catattgaag gttcattttt accagtttct atagcattta
     2161 tagcctgtct agatgttcca actaatctcc ctagttgttc ttgtgtcaat cctaattttt
     2221 ctcttaatat ctttatttta tttttcatat ttgctccatt catgtaaggt taactttaca
     2281 tcttaaatgt aacatttttc aacattactg tcaagtcaac cttacattta tatattaaat
     2341 tttcttattt aactattttt ttactaaact ttttaaataa taataaaatc catattaatg
     2401 atataacaat tgaaataaac atatagacat aattatcttc cttaaatgcc ttcgatatcc
     2461 aaaaagttgg taagaagaac agaatatatt gtgctttatt taaaataaaa aatggtgcta
     2521 aaatacccat cataaaaaca cctgagaatt tcgtaattac cattccttct agtttatttg
     2581 atgataaaga aatcactaat attgatataa taactccttg taataaaact aataaagaaa
     2641 ttgataagtt taacaagaga gaaattttag ttagagaaaa aaagataagt agaatcatag
     2701 taataataaa tgctaagata cctggtattc caaatcgtga aataagataa ccagttttac
     2761 caagcggagt tactattaaa tatcttgaaa tgctgtcatc aatttctccc aatatgacca
     2821 ttgtagaagc aaaaaagaac ataaaaggtg taataaaagc taaaagtaaa tcaaacatca
     2881 agaaatacgg ctctaaattt aattgataat aaaacttgtt aagtgatata ttctgtatta
     2941 atggtattcc aaattttata aatactccac ataatattgg agaaacacat actataaaaa
     3001 gcatagcatc tctcttaatt tgttcaaact cttgtttaaa cgcatttata atctttatca
     3061 tagttttact ccacctacct ttttccaact gctacaaata aaataataag ttataaaata
     3121 aattattatt ataattgata ttaaaattaa aatattaatc attataaaat ttttatctcc
     3181 actaataaga gaaatacata tattaaaagg gtacaaattt gcatatgatt ttgtgtcaag
     3241 aagtacattc agtattggtg gaataaagca tataatctca ataggaatac ttaaaacaat
     3301 aaactgattc aaactagatg ctttacttgc aattatcaat ccaagcaaag aaaatataat
     3361 agaagaaaaa aaggtaccta tagttgatat tataatatta tctaaattta atgtaatagc
     3421 aataaacata gctacaatac tagatattac tcctaatgaa attacctttg ataaaatata
     3481 ctcagatact tttactggag atacagctaa ggaatttaaa accctttgac ttttctcaag
     3541 aagtacaata gctcccataa aaaacaatcc cattgctgct gggtcagaat aaatcataat
     3601 tatagctatt ttttctctca taaaagttgg aaatacattg ataatacaaa tatatataat
     3661 acttaaaaaa agatataaaa aataaaaacc atacttaaat tgaaacttta tatctcctaa
     3721 aataagattt tttagcctca ttgtaaattc ctccctgtta tctcaataaa aatatcattt
     3781 aatgttggtt cactactatg tattgataaa agtttatttt tctcaataag catattcaaa
     3841 tttttatcat tagcagtatt gttcagaaaa cattcagaag ttttttctcc attatcaaag
     3901 taagtatatc taactttaat agctcctttt gacataataa gattatgtgg tgtatctaaa
     3961 gcagatattt taccatttac aatgaacgct actctgtcac aaagctctgt tgcatctaac
     4021 atgttatgag ttgttaaaat aatagtctta cctttcgatt tttctgataa aattatatct
     4081 ttcataacct tgctatttga tgggtcaaga ccacttgttg gttcatctaa aaataaaata
     4141 tctggattat gtaataaagc ttttataaaa tttaatcgag atttcatacc ttttgaatac
     4201 tcagataccc ttttatttga ttcattttct agtcctactg attttaataa ctcatcaata
     4261 gatagtagtt ttttagaata tagagaccca aaatatttta aattttctat tgcagtcaac
     4321 ttttcatata aactaggaaa ttcaaaatca actcctatat tttcataaaa tttatttgag
     4381 tgtcttttac tctctactcc atttacaatg acacttcctc cataattagt tatcattcca
     4441 attagaatct tttgtaatgt agattttcct gcacctgatg gtcctaaaaa tcctagtatt
     4501 tccccttttc ctacttcaaa gttaatattt tgtaaaaaat ctctatctgt ataactaaat
     4561 gataaatcat caactttaat cattcaatat caccctttca aatttttgac tattttgact
     4621 atatggtcaa taaaatataa tttttttgtt tgttaataat ttttaataaa agttattagc
     4681 aaacaaaatt taaattctat tccataagtt gtataactaa tccgtttatc attaatctta
     4741 aagcatcttc aaaacaatta cttccaacct cgcgcttata tagcatggta agaaaaactg
     4801 ctctaaatgc tccactaaag ctttctatgt ctttattttt tgcatttggt atgcaagata
     4861 taatctctcc aatttcaaag tcatcatgtc taaagtattc ttcaattata gaatctggta
     4921 actttctcac caatatctca aattctccat ttatcatgag atttaaaatt gaagtactat
     4981 caaccatcct atagaatttc ataaatatat ctgtcaattg ttcacatgta atactttcat
     5041 ctactctatt tatctcatat aagatttctt tttgtataga gtcatgtata tctcttaata
     5101 cctcaaaaaa gagtaattct tttgattgaa aaaatagata aaaagttcct tttggtattt
     5161 ttacccgttt tacaagctca tcaacagtag tttttctaat accatattgc tccatacaca
     5221 atcttgcttc ttctttaagt ctttttatta tatactctct ttctttatca gaatatgatt
     5281 taggcatctt ttccctccag tttgactaaa ataatttata tagtcaatat aatacttttt
     5341 ctatctaatg tcaatagtat tattagtata tttaaaaata agcttaaaaa ctttcgaaaa
     5401 ttctgaattt tatatagcat tttttattat ctagtgttat aattaagata taaataatat
     5461 attattctaa ttttcatatc tatttgctta ttttttaact tagtttctaa tagaagtaat
     5521 catatttatc tttttaaaag tttcaacttt aataaaaaat aaaggagttg attttatggc
     5581 tactactaca aaaaaccgtt tttacgatta tgttcaagaa aacttaaata aatatgttga
     5641 agtacattct tactttagca gttgtcctct atatggtgaa attatagaag cagatagctt
     5701 atcaattaca ctttgctcaa gatatataga tgaatctaat ccaactttaa atgaaactta
     5761 cacattatat ttaccattaa gttcaataat atctattaga acactataaa aagttaatta
     5821 aatatgggtt atttatatat aaaatgtaag attttctata tttctatata aagtaaccct
     5881 tttttatgtt tttaaactta tactacataa atttaaagct gaaaacaaaa ctttacaatc
     5941 attgattatc ttatttatca ataatttgaa gtaattattt tcagctttta ttaagataag
     6001 ttactaatac acactaatat atgttaatac ttaagtactc tatttagcac tttttaattt
     6061 tactaactct ttaaatgcat ttttatttgc tcctccacca acttgagtta ctttttctaa
     6121 agtcttattt ttaagaactt ctttttggct atcagctaat ttatcaccaa ctaaaattac
     6181 tggtgaccca gtttttgctc ctaaaggacc aactgccaat ccatctatta aatcattttg
     6241 attcttgatt ccatttttaa ctacaaatgc attttttaaa gtatcttttt tataaaaatt
     6301 atctattatc tttgaattag tttcgtctct actactacca gataatctag ttggattttt
     6361 taacttactt tctagattgt ttgataggga tgctgttcct ccaataatat aagatttctc
     6421 tatatttaat gaatttagaa attcatcagc tccttgcaat tcgctctttt catttgcaag
     6481 tatgattggt atgttatttt ctgctgctgc agcgcctaca ctaattgcat cagctaatcc
     6541 tttcactcca tttacaactg ctatttgaga tacatttttt ataccattta acttttgagc
     6601 taataataaa cttgtcttgt atctatcact tcctgatatt ctctctatcg atatatttaa
     6661 gctttttaat tgttgttcaa tttctttact tatagaattt tctccaccta ttaggtatac
     6721 tttcttagca cctaatctct gtatctcagc tttagttttt gaatttaaag cttctttttg
     6781 tgtcaataat attggtgaat ttatagcaga tgtaaaagga gttgctgcca atgcatctac
     6841 tattgaactg ccatttacta aaactacatt ttctgaagaa ttgaaaccat tttgactaac
     6901 tttgatagca gtttcatatc tatcactacc aattaaagac tcatttggga tactctcacc
     6961 agaagacttt tctgtaacag cttttatagt aaaattacac ggatagaagt tactatctct
     7021 atttatatca aagaattttc cagtattatc tatgatataa ctttcaccta aattagcttt
     7081 tgctttaaat gtatcatatc ctggtacatt tgactcagaa cctatagttg actctccatt
     7141 tttcccctct atactgacta caattgcacc ctttccttct ggtatatcaa aagaatcaat
     7201 tggaactgtt atgtatcctg aatattgggc agtaccttct tttaattttt tcatattgtt
     7261 ttcttgaggt ataccattta ctactggagc ataatatact tcatacttag ctcctatact
     7321 ttcagttaag aacattacag attttagagt ttcatcgcct ctattgaaat caaatacatt
     7381 tgcagctgtt atcttttttg aaattaaagg tacaatactt gcatagtcat gttgatacat
     7441 cttctcatca tcatttggtt ttttaccacc tttgattgaa aaattatcta tatcagacat
     7501 aagtgtcttg tcttcataag atatccagaa atatccaccc tcagaattat aatctcccca
     7561 gctatttttt attaaccaag caccattttt tgatggtcta atatttgcat taaaattatc
     7621 tttagaatag ttatcatccc aacctacaat tgaaatagca tgatttgtat taaaactttt
     7681 tgttggacaa taatatgaat tttggtcatc actttgatat ttaatatctt cataataacc
     7741 agatgttaca gctccatact gtaatatagc attttttgta gaatttatgt cattgcttac
     7801 acacacagca tctgtcacat taaaaacagt aggtgctgta ttcatattag ttggtttttt
     7861 tgctccttgt gctttagata ctctaccatt gtatgggata tctttttcta atttaggacc
     7921 tgaccaagat gttaaatatc ccatagcttc ataatttaaa gcaccattca tatcatttac
     7981 agaccatcca tattcaccat ctgaagccca ccatcttaag tgttcttctg ataaatcata
     8041 gtctccataa ttatttaact ttaagaaact ttctaaggtt gccattgcag caaaatccca
     8101 acagattcct aaatcctctt gattttttat tgatgtcata agacctaatt ttctagggtc
     8161 ataagatgtt ggagctgttc ttcctttact ttggatttga gtaccttcca actgatatgg
     8221 atttggtata atcccattat atttttctgt acatccattt ttagcatcat ttagatattg
     8281 tttatacgtc tcactgtatt ctgctttttt tatctgattc aatgtattgc tattgtcagc
     8341 tgatacagga attgttgaca ttactaaaat agatattaaa aacagcgcaa caaaccttgt
     8401 tacttttttt gaagtaaatt ttttcacgct ttctccccct tattttgagc taatttttaa
     8461 aattataatg ttatatatga ctaaatatca agtatttaca aatatacaat catttaaatc
     8521 aattatacca aagttttcct ttataaataa ataaaataaa ataaaaaact gaatttattt
     8581 ataataaatt cagcttctca ttttcttttt atatgttttt atctaaatcc tattacagca
     8641 gctcctaaag ctcctattat ttgagtatct tctgcaataa aaattttttt acccagtatt
     8701 tcttctatca tttttactag ttctttactt tttgcaagcc ctccagtaaa tgcaacttca
     8761 tctattacct cacctcttgc taacattgag gtggatttat ttgcaataga tttaagtatc
     8821 cctgctgcta cctctccagt agaaatgttt tgagctagaa gacttacaat ttcagactca
     8881 gcaaaaactg tacacatact agatatatta actggttcat atcctttagc taatgtatct
     8941 atactttcaa tatcgcttcc taaaaggtta gaagtaatct ctaaaaatcg accagttcct
     9001 gcagcacact tatcattcat tataaagtta aatacatttc catctctatc taaatttata
     9061 actttactat cttgaccacc tacatctaaa atagtcctta tgttcttgtt taaaaagtaa
     9121 atacctctag tatgacaagt tatttctgtt actttcttat cagcaaaatc cattactccc
     9181 cttccataac ctgtacctac cacctttttt atatcttttt tgtcaatttc actacttagc
     9241 aactcataga cctgtttaga tgtactttta gggctccatc cagttggtat aattattttc
     9301 ttaataattt tttcaccatc aaatagcaca ccctttgttg ccactgaccc tgagtcaact
     9361 cctatactaa acatagttcc tcctactttt atttattcct acaacatctc tacaaacgct
     9421 tcaaatctag ttcttaattg ttcagtatct gattttgaat aatcagtttc aagagacata
     9481 aatggcttat ttttttcttt tgtaacaaat tctcttattc tataactttc tatattaaat
     9541 gtatgacatg cttgtaaaac tacatctatt acagcatcta cattatattc atctattatt
     9601 ctatctaata attcaatacg attgtcatta ttcatcatac aagaacaacc tatatttata
     9661 tatttttgtg ttaaagcatc atatacatct tctatttctt catcaaccaa taaatcgttt
     9721 gatctaatag ctccacaaag ttcatatgca acgactgatg cccctgcatc ttctaaagtt
     9781 ttaactattt tttcgcttat tccaccaata ggagaacctg ttatcaatat tcttggctta
     9841 tctttttgaa caggacattc acctttttca tatctttctt tcatatcatc tataactttt
     9901 cttaaagaat gtttaagttc tgcacggtca aatttaaaac ctgcttgata taaaacatta
     9961 tgtaactcaa ggcctgtaag tgcagatggt tgtaatttac caagtgcata aaactctttt
    10021 aaaagttctc tttcttcatt tttatctttt atagcagttc ttatatcttc ttcagttata
    10081 gttacaccta aagatttctc aacctcttct tttaatctta tcatttcatc tttccaaagt
    10141 ttataggcaa attctccttt tgaagtattt ggcaaatgca ttacatatgt tggcttaact
    10201 tttgcaagtt cttcatacat ttttttctta ccatcacaag tggtttctcc tatcaacaaa
    10261 tcagaaaaat agaaataagg acaggtatct gttattgcat gtccatagct tgatttaatt
    10321 aaaggacaaa gatttcttgg aagcaccctt tctgcatcag gtattggctc ttcactaacg
    10381 ccgcatacac ctactacagt tgctccagct gccattggaa tttcaactgg agtaaatgta
    10441 cagaaaactc caaccatctt tttcccactt tccttaagtt cctttgcatt tataaatcct
    10501 ttttgtctag cttcattgaa gctatcaaat tgttttggca aatttgtcat gaaaatattc
    10561 ctcccaatta cttctcttca taattcaatc tcttcgtaat tcaatttgta tcttaagcaa
    10621 taaaaactaa atttttccat taaaatacaa acctctacat tagattctat catgctttac
    10681 cttttatcca taattgtttt tatcaatgca aaattagata catatagata tattgtataa
    10741 ctaatttttt atgttataat ttttcttata acttaagctc tcgaatcctc aatctataag
    10801 cagacacagc agtttcacat aatttatcaa ttaattttcc attcacataa aatcctacaa
    10861 atgctcctat tccaggcatt agttgtaaaa acttagctaa atctaaataa tccctatact
    10921 cttgttgaaa tgttctccaa tcaaaatcat tgatgtcttc tggtagacta tttacatatt
    10981 catcccagtt attcatcaat ttaaaagttt tgtttatatg ctgttgacta gaaaaagcca
    11041 actgtaatat atttaatata taaatccttt ctctataatc tttaacatca tatccatata
    11101 ttgaagctac atcatataaa aatttcactt taatacctaa aagcaaagga aaatcagata
    11161 aacctaagag gattcctcct gcccctgttc ctgccccttc aatcattgct gtagtcttgt
    11221 aagttttagc tttttctaaa actaattttt ctctttcttc taatgacata tttctaagtg
    11281 gtggctttgt agcatattta tatccaaaca gtacggtttt tgtcattact tttatagaag
    11341 aagtaattat ttcatgatat ttatctggca atactccatt aaatttattc tgtataccct
    11401 ttgatgcttt gtcaaatata gaaggtttct ttaatatatt tttcttccat tcttcaagtt
    11461 cttttttgac tagtttaata taaacaacat cttcataatc tttcacattt tttctcctaa
    11521 tacaatgtat ttttatcaat ttaatagctt aatttaaagt ttaaatattt ttatctatta
    11581 aattaaacta ttcaaatact ctttagtttc tttatctgca aaactagctt ctactgcatt
    11641 tctataaatt attttatagt cttctaatcc tagtttaaaa gtatcaaata taacatttag
    11701 ttctgaactt aaatttatat ctgatacagt tctattatca gtatttatac ttacatgtaa
    11761 attatcttta taaaaagtat aaaatggatg tgcttcataa gaatctactg tcttagtttg
    11821 tacattacta gttggacata attcaagcaa tattttttta tctttaacta aattatacgc
    11881 ctttttatgg tctttaattt ttaccccatg accaatccta tccgccttta aaatatttat
    11941 agcatccaat acattttctc cacttgcagc ttctcctgca tgtattgtaa ctttatatcc
    12001 atattctctt gctaatttaa aaacatcttt atattcctta caaaatccct ctttctctgg
    12061 accacacaag tctactgcta caacaccttt atttacaaat gatttccctt cttcaattac
    12121 taataatgcc tcttttgagg tcattgttct catacaacct aatattaaat tgccttttat
    12181 atcataaata ctttcagctt ctctaattcc ttcaattata ccctcaatta tatttttaac
    12241 actcatacct ttttgggtgt gaagtaatgg tgcaaatcta atttcaatat attttacatt
    12301 ttctctactt gcatcttcca aaagttcaaa agtaattctt tttaaatttt ccttagattg
    12361 cattactttt aagggtaaat caaatttttc taaatattca tctagtgaag tacaattaaa
    12421 agaaaccttt gctaattttt ttatttcatc catattattt gaaggtaaat ctattttctc
    12481 tttaatagct atatcaagca tggtttctac tctaacactt ccatctaagt ggcaatgtaa
    12541 atctatctta ggtaaatttt caaacatttt atcctcctac tgtacttata aataatacta
    12601 tcaacagatt tttaagtctt atttctatta tatcaatata ctacggtttt taattctaaa
    12661 aaagtcatat ctcattaaaa tattttaatt taatttatga ctatctaatt atatctactg
    12721 attcttttta atatacattt attaatatac atttgataat aaaatttcaa tacataagaa
    12781 tttaagttat ataattaaaa ctttgaatta caaaggagga cttattttga ttaccttaag
    12841 ttttgatata gtgcaaacct tgacactatc tatattcttc tttttaattg gtaatttatt
    12901 gaagaataaa gttaactttt taaataattt ctgt
//
LOCUS       DAEMGG010000111        12800 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEMGG010000111 DAEMGG010000000
VERSION     DAEMGG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.112"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            76..789
                     /gene="ispD"
                     /locus_tag="KPK24_002927"
     CDS             76..789
                     /gene="ispD"
                     /locus_tag="KPK24_002927"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG2300579.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVKETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            786..1271
                     /locus_tag="KPK24_002928"
     CDS             786..1271
                     /locus_tag="KPK24_002928"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG2300580.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    1366..1574
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1638..3353
                     /locus_tag="KPK24_002929"
     CDS             1638..3353
                     /locus_tag="KPK24_002929"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG2300581.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            3475..4920
                     /locus_tag="KPK24_002930"
     CDS             3475..4920
                     /locus_tag="KPK24_002930"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG2300582.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            5358..6839
                     /locus_tag="KPK24_002931"
     CDS             5358..6839
                     /locus_tag="KPK24_002931"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG2300583.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            7060..7215
                     /locus_tag="KPK24_002932"
     CDS             7060..7215
                     /locus_tag="KPK24_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300584.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            7360..8757
                     /locus_tag="KPK24_002933"
     CDS             7360..8757
                     /locus_tag="KPK24_002933"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG2300585.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHSQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            8742..9155
                     /locus_tag="KPK24_002934"
     CDS             8742..9155
                     /locus_tag="KPK24_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG2300586.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            9261..10019
                     /locus_tag="KPK24_002935"
     CDS             9261..10019
                     /locus_tag="KPK24_002935"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG2300587.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEVLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            10032..10769
                     /gene="rlmB"
                     /locus_tag="KPK24_002936"
     CDS             10032..10769
                     /gene="rlmB"
                     /locus_tag="KPK24_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG2300588.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            10772..11299
                     /locus_tag="KPK24_002937"
     CDS             10772..11299
                     /locus_tag="KPK24_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG2300589.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            11364..12011
                     /gene="sigH"
                     /locus_tag="KPK24_002938"
     CDS             11364..12011
                     /gene="sigH"
                     /locus_tag="KPK24_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG2300590.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            12085..>12800
                     /locus_tag="KPK24_002939"
     CDS             12085..>12800
                     /locus_tag="KPK24_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG2300591.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVL"
BASE COUNT         5321 a         1323 c         2530 g         3626 t
ORIGIN      
        1 ttaatcagat ggttaaaata aaaatattaa aagttttaat aaatgatggt taaaaataaa
       61 atacgaggtg ataccatgta tagtgttata attgttgcag cgggaagcgg aaggagaatg
      121 aacttagata taaacaaaca gttcataaaa ttaagagaaa aagaaattat tgctcataca
      181 attcaagttt tttatgaaaa tataaatata gatgaaatag tggtatgtat aaaaaaagaa
      241 gaagaagatt tttttaaaga aaatataata aataagtata actttaaaaa tataaaaata
      301 gcatatggcg gaaaagaaag acaggactca atatataatg gtttaaaaaa attagataaa
      361 aattgtgata tagtacttat ccatgatgga gcaagaccat ttgtcgacca tagaataatc
      421 aacgaatcta taaaggtagc taaagaaaaa aaggctgtgg ttgtaggtgt tccagtaagt
      481 gatactataa agatagtgtc agatggaaca gtaaaagaaa ctccagaaag aaatttgctg
      541 tgggcagcac aaacccctca aacctttgaa tataatctca taatagatgc ttatgaacaa
      601 gcatataaga acaattatta tggtactgat gattcaatgt tggtagaaaa tataggtcaa
      661 agtgtcacta tggtaatggg ttcttatgaa aacataaaaa taactagtcc agaagattta
      721 aatatagcag aacaaatatt aaatatggaa aaaagagatg aagttaacag tagaaggaga
      781 attatatgag aataggatta ggatatgatg tacacaaatt aacagaggat agaaaactta
      841 taatcggtgg tgtagagata ccacacgata aaggtttgtt gggtcattct gatgctgatg
      901 tattgataca tgcaataatg gattctattt taggtgccct agcattagga gatataggga
      961 agcatttccc agatacagat gaagaatata agggagcaga tagtatgaaa ttattagaac
     1021 atgtgtataa tctaataaca agtaaaggat ataaaattgg taatatagac agtactataa
     1081 ttgctcaaag ccctaagatg gctccatata ttgagagtat gagaagtaat atatcaaaag
     1141 ttttaaacac agacattgat aatataaaca taaaggcaac aacagaagaa ggtcttggtt
     1201 ttacaggagc aaaacaggga atagcttcac aaagtatttg tttattattg ttgacaagtc
     1261 agaataatta atatattatt agtaataagt ttaatggata tcaagtaata ggcctatata
     1321 gaatatctat aatgtatgaa tttaagtgaa attaatataa atataaaatt agctatgata
     1381 aagaaatagt aaaatgatga aatcttcaca gagagaaaac ggtggtgaga gttttcaaga
     1441 ggaagtcttt gaacctgtct tttagcttaa aggtataaaa atctttacgg taggaaccgt
     1501 tatattccca aaagagagct attcatatag ctaactagag tggtaccgcg gaaattaaac
     1561 ctttcgtctc tatacttgta tagagatgag aggtttttta tattttcagg aagttaatat
     1621 tcaggaggaa tctattaatg aaaatgtcaa aaatgtttat gccgacatta aaagaaatcc
     1681 cagcagatgc tgagattaca agccatcaat taatggttag gtcaggcatg ataaaaaaaa
     1741 tgacttcagg tgtttataac caattaccaa tggggttaag agtgttcaaa aaaattgaac
     1801 aaataattag agaagaatta aataaaaaag attgtcaaga aatactttgt gcagccttac
     1861 ttccttctga attatggaaa gagtctggaa ggtggactgc tatgggtgaa gaaatgttta
     1921 gactaaaaga cagaacagaa agagagtact gtcttgggcc aacacatgaa gaagcattca
     1981 ctgatataat aagacaagaa attacttcat ataaacaatt gccactaaac ttgtaccaaa
     2041 tacaagtaaa atatagagac gagagaagac caagatttgg tgttatgaga actaagactt
     2101 ttacaatgaa ggatgcatat agttttgatg tagatgataa aggtttagat aagtcatatc
     2161 aagatatgtt tgatgcatat gtaagtatat ttgatagatg tggattggag aatagccctg
     2221 tacaggcaga ttcaggagct ataggaggtt ctacatcagc cgaatttatg gttaagtcag
     2281 aagttggtga agatgaagtt gtattttgta gtggatgtga ttatgcagcc aatgtagaaa
     2341 gagcagaatc ttgtaatcta gcatcacaaa aagaagaaat gaaagaatta gaagaggttc
     2401 atacaccagg agcagctaca ataaaagagt tagaagaatt tttaaaaact tctccagata
     2461 agtttgctaa aactttagta tatgaggcag atggaaaaac agttgtagtc gttgtaagag
     2521 gagatagaga agttaatgaa atcaaagtat ctaatgccat aggttctgtt atagaatttg
     2581 ctcttgctac agatgatgta gtgagaaaag taactaatgc agaagtagga tttgcaggtc
     2641 caataggtat aaatgctgac tatgtattta tagataaaga aattgttgaa caaagaaaca
     2701 tagttgttgg ggctaataaa actgagtacc atataaaaaa tgctaactat ggaagagatt
     2761 ttgaaggaat agttggagat tttagaaatg ttcaagaagg cgacaagtgt atagtatgtg
     2821 gtaagccact tgaaatagct agaggtgttg aagtaggaca tatatttaaa ctaggaacta
     2881 agtactcaga atctatgaat gcaaacttca tagacaaaga tggaaaatca aagcctatag
     2941 taatgggatg ttatggaata ggtgttgaaa gaacagcagc agctataatt gaacaacata
     3001 atgatgagaa aggtataata tggcctttgt cagtagctcc ataccatgtt gtgatagttc
     3061 cagcaaatat gaaaaatgaa gaacaaatat caatagctga aaatatatat aatgatttgc
     3121 aagctatggg tgtagaagtt ttattagatg acagagatga aagaataggt gtcaaattca
     3181 atgattcaga attaatagga atacctatga ggattacagt aggtaaaaat attaatgaag
     3241 gtaaagtaga atttaaactt agacataaag aagataaaga gataattgac atagaagaaa
     3301 taaatgaaaa agtaaaggca gagtttatca ggaataatgt taggttagga taataactta
     3361 aataagatat tgtatagata atcaaagttg tactttatag acaccatttt atctatatat
     3421 aaggatataa aatggtgttt ttttataaaa caaataaatg aggaggtaat taaaatggca
     3481 aaaaatgaaa aacaatttgt tgaagaaata acaaagatgg aagatgattt tccacaatgg
     3541 tacacagatg taataactaa gactgactta gttgattatg caccagtaaa aggttttatg
     3601 gttgtaaagc catatggata tgcattatgg gaaaaaatgc aagaatttat ggataaaaaa
     3661 tttaaagaaa ctggacataa aaactgttat tttccattat taataccaga gagtttatta
     3721 aacaaagaag cagaacacgt tgaaggtttt gctccagaag ttgcatgggt aacacatgga
     3781 ggaaataaaa aattagaaga aagattatgt gtaagaccta cttcagagac tataatatgt
     3841 acaatgtatg cgaaatggtt aaaatcatat agagaattac catatcttta taatcaatgg
     3901 tgctcagttg ttagatggga aaaatcaact agacctttct taagaacatc tgagttttta
     3961 tggcaagaag gtcatacatt acatgaaaca gcagaagaag cacaagaaga aacaatacaa
     4021 caattagaag tatataaagc gctatgtgaa gaattacttg caatgccagt tgtagctggt
     4081 caaaaaagtg aaagtgagaa atttgcaggt ggtgaaagaa catatactat agaagctatg
     4141 atgcatgatg gtaaagcatt acaatcaggt acaagtcact tcctaggtca acactttact
     4201 aaggcttttg atattacttt tgcagataga gaaggaaatt tagctaatcc ataccataca
     4261 tcttggggag catctacaag acttataggt ggacttataa tgactcatag tgataacaga
     4321 ggtttagtat taccaccaag agtagctcca attcaagttg ttatagttcc tatagctgct
     4381 aaaaaaggaa atgtaatgga gactgttgat aaaatatatg ctgatttaaa agctaaggga
     4441 gtagctgtag aagttgatga tagagataac tatacaactg gatggaaatt taatgagtgg
     4501 gaaatgaaag gtgttccagt aagagttgaa attggaccaa aagatataga aaataatcaa
     4561 gctatggtat ttagaagaga tactttagaa aaggattcta tgcctttaga aggattagct
     4621 gatgctatat gtgatttatt tgatgttata cacaatgata tgtttgaaaa agcaagaaaa
     4681 catagagaag acaatacatc tatagttgaa aatatggatg agtttagaaa agctttagaa
     4741 gaaaagccag gattcataaa gactatgtgg tgtggagatg cagaatgtga agctaagatt
     4801 aaggaagaaa caggagcaac tataagatgt ttaccttttg aacaagaaaa cttaggtcac
     4861 aagtgtgtgt actgtggaaa agaagcagat agtatggtag taatggcaaa agcttactaa
     4921 aatacattta aataaaaact ggggtagaaa gtatttaata ctttatccca gttttttgtt
     4981 ttttatgagt aataaaatgt atttgtaact atgtcataag ataagtcata gatacttata
     5041 ttaaaaagaa tataatagag aactattttt aataattaca tattaatgaa agcaaaatat
     5101 tttgttatat agattttcaa taaagcaaac aataaatata aatgaatatt aataaggagt
     5161 gcttttaatt tttagaaaat aaatgactta aaagaaagat ttatgaaatt aaaactagga
     5221 ttttctgata taataaacat gtgtattttt aaataaaaag gatattatta ttagatttga
     5281 ttaaaaataa acttgcaaaa caatgtacta gtttaatatt atagttatat tgaataaaat
     5341 atcaggagga agtaaaaatg aatgtaagag taaggtttgc accaagtcca acaggatttg
     5401 tacatatagg tagtttgaga actgctttat ataactattt atttgcaaaa aaaatgggcg
     5461 gagaatatat attaagagtg gaagatacag accaaagtag attagtagaa ggtgctatag
     5521 agaatatgct taatgctatg aaatgggctg gtgtcaacca tagtgaaggt gttatattag
     5581 atgatagtgg aaaagtagta caaaaaggag aatatggtcc atatattcaa tctcaaagat
     5641 tagatatata ccaagaatat ataaagcaac ttttagatag tggaaaagct tattactgtt
     5701 tctgtacaaa agaaagactg gatgaagtaa gagatgctca aagagcagca ggagaaacag
     5761 ctaaatatga tggtcattgt aagaatcttt ctaaagaaga agtagaagcg aatataaaag
     5821 caggaatacc atatgttata agattaagat tgccagagaa tcatacaatt aagtttacag
     5881 acttagttag aggagatatg gaatttaata ctaatgactt agatgaccaa gtattaatga
     5941 aaacagatgg atttccaact tatcattttg cagtagttgt tgatgactat ttgatgaaaa
     6001 ttacacatgt tataagagga gaagaatggg tatcatctac tccaaaacat gtttatttat
     6061 atgaagcttt tggatgggaa gcacctgtat ttgtgcattt accaaatata cttaataaag
     6121 aaaagaaaaa attaagtaaa agacaaggtg atgtagctgt tgaagacttt aagaaaaaag
     6181 gatatttacc agagggatta gtaaactatg tagcattagt tggttggtct cctgaagata
     6241 atcaagaatt gtttacaatg gaggaattag aaaaagcctt ctctgtagaa agagtatcta
     6301 aaagtggtgg agtatttgat actgaaaaat taaattgggt taatcagcac tatataaaag
     6361 atggtgatga tgcttatcta acagacttag caataccatt tttaatagaa gatggattta
     6421 taacagaaga agaagctact aataaatatg atttcttaaa gagtatgata tcagtactta
     6481 aagagaaatt acaatatgta aaagaagtta ctgaacatgc aagtatattt tttggagata
     6541 aaatagaggt agaaactgaa gagggaagag agttcttaag attagaacat atacctactt
     6601 taatagatgc tttgagagaa aaaatagaaa aaacagaggt attaaatgct gagtttgttc
     6661 aagctatgct taaagaaata caaaaagagt acaaaataaa agggaaaaat ttatttatgg
     6721 gctcaagaat aattttgaca gggcaaatgc atggtccaga tttaccaaaa gtaatggaag
     6781 ttttaggaaa agaaacttgc ttaaatagaa tagcttatgt aaaaaataat atattatagt
     6841 cataaaagtc cgctaattta gaagcggact ttttttgtgt tagtatttgg tgaaaatatc
     6901 tgcttaagaa aatgaaggta tttaaggtaa taaatttaat atataaaaag caaagcaaaa
     6961 atataaataa aatatgaata ataaactaat aaaatattaa gcatagaaga agggaatcta
     7021 ttattttagt ttgatttaat tattaagggg gtaattacta tgtatagtaa tattgatgat
     7081 gttaaaaagg aattaaagga gctatgctta gaatatgtta caattttaga aaaattaaaa
     7141 gatgaaaaaa tgattacaga agaaactttt gaaaagtgct cttctcaaaa aaaaatattt
     7201 ttagaagaac aatagaggat ggtaatagta ttattattat gtagtttgaa agatagtacg
     7261 aaaaatgata gtaatactat tatttattgc aattttcaac tataatataa tatataatgt
     7321 taagaagata atttataaaa atacagtgag gtgatgagtg tgaaagttta taatacattg
     7381 actagaacaa aagaagaatt tgttccatta gaagaaggaa aggtaaaaat gtatgtgtgt
     7441 ggtccaacag tttataacta tatacacata ggtaatgcaa gaccatttat aatatttgat
     7501 actctaagaa ggtatttaga gtatagagga tatgatgtaa cttatgtaca aaactttaca
     7561 gatgttgatg ataagattat aaatagaagt catgaggagg gaatatctcc tgaagaagtt
     7621 gcggctaagt atataaaaga atattttgta gactgtgatg ggctaggaat aaaaagagct
     7681 actgttcatt ctcaggttac agataatata caacagatta tagagtttat aaaagagttg
     7741 gaagataagg gatatgcata tgcagtaaat ggtgatgtat attttgatac aaataagttt
     7801 gaaggttatg gaaaactttc aggtcaaaaa caagaagatt tagaggcagg ggctagaatt
     7861 gaggttaatg accaaaaaag acatcctatg gactttgttc tttggaaggc aaaaaaagaa
     7921 ggtgagccag gatgggatag cccttgggga gaaggtagac caggatggca tatagaatgt
     7981 tctgttatgt ctaagagata tttaggtgaa acaattgata tacatgcagg tgggcaagat
     8041 ttaacattcc cacatcatga aaatgaaata gctcaaagtg aagctagaag tggaaaaaca
     8101 ttctcaaaat actggatgca taatggatat ataaatataa atgatgaaaa gatgagtaaa
     8161 tcaaaaggta atttctttac agtaagagat atatcaaaat tatatgattt agagatagta
     8221 agatttttca tgttatcagc acattataga aatccagtaa actttagtga tgagatgtta
     8281 aatcaagcta aagcaggtct tgaaagacta tataatacta aagaaaaatt agaatttacg
     8341 ctaagtaatt tagtagaatc tcctttaaca gaaaaagaag tagagcttgt taaagaatta
     8401 gatgacttta gacaaaaatt catagatgct atggatgatg atgttaatac agcagacgca
     8461 gtaagtgtaa tatttgaatt agctaagtta ataaattcta atgtagatga aaattcttct
     8521 ttggagtttg ctaaaaaatg cttagatgaa tttaatgaac ttacaggagt attaaatatt
     8581 gtaaataaga aaaaggatac tgtattagat aaagacattg aagaattgat acaaaaaaga
     8641 actgatgcaa agaaaaataa agagtttcaa ttagctgatg atattagaca acaattacta
     8701 gatatgggaa ttgtattaga agacacaaga caaggtgtta agtggaaaag gatataaaaa
     8761 tggaaaaaac tgaattagtt actatgtcac cattagtttt agcatactta ggtgatacag
     8821 tgtatgagac atatataaga gaatatttaa ttagacagaa tacacaaaga aaagttaatg
     8881 atttgcataa actagctata aagtatgtaa aagctaaagc tcaggcaact ataattcatg
     8941 aaattgaaat tgagttaact gaagaagaga gtaaaattta taaaagggga agaaatcaaa
     9001 agtcaaatac ttctcctaag aatgcagata ttatagacta caaacatgca actgggtttg
     9061 aggcattagt tggatatttg tatttaaata atgaaataga gagacttcaa tatattatca
     9121 ataaaggaat caaaattatt gaaagagata tgtaaatatt aaagagtgtt gtcttaataa
     9181 taagtaataa ggcaacactt tttttataac taatgtataa taaatatata gaaattagta
     9241 atgagaaagg ataaaaagtt atgaaagtaa aattaatatc acatacacct gaaccagaaa
     9301 aagtaatagc tatggcagct aagctatgtt attcaccagt tgggacagac gaaatagaaa
     9361 aagacttaac agatgaaagt atagagaaat ttttaaatat gttattaagt ataggtcatg
     9421 ggtcaatatt agaacatgct tcatttacat tttcaataga aggtatttca agggcatgtt
     9481 cacatcaaat tgttagacat cgtatagcta gtttttcaca acaaagtcaa agatatgtta
     9541 aattagaaca atttgagtac ataattcctc cagaaataga aaaaatagaa aaggcaaaag
     9601 agttatttat agactccatg aaaaaagacc aagagaatta tgataagtta gttgaaatat
     9661 tgtttgaaaa tcattataat gacctaataa aaaacggtaa aaatgagaaa acagctaaaa
     9721 gacaagctga gaagaaagca atagaagatg ctagatacgt atttccaaat gcatgtgaaa
     9781 caaaaatggt atttactata aatgctagaa gtttgtttaa tttctttgaa catagatgct
     9841 gtgaaagagc acagtgggaa ataagaaatt tagctgtgga ggtgttaaga gaggttaaaa
     9901 aggttgctcc tatattattt aaaaagactg gtccaagttg tgtaaatgga agttgtccag
     9961 aaggaactat gacttgtgga gatatagttc aagttagaga aaaatttaag gccttgtagg
    10021 aggtgaataa tttggcgagt atagaaggaa gaaatccggt aatagaagca ataaaaagcg
    10081 acagagaaat agataaaata ttaattgcaa attcagctaa agaaggttca attaagaaaa
    10141 taataggaat ggctaaagat aagaatataa ttattcagta tgttgataaa cataagttag
    10201 atgaagtaag tacaagtcat tcacatcaag gtgtaatagc ttatgcaagt gagtataagt
    10261 attatgaatt agatgagcta atagatttag caaagaataa agatgaagac ccatttttca
    10321 ttatattaga tgagataaca gacccacata atttgggttc aataataagg acagcagatg
    10381 cagtaggagc acatggagtt atcattccaa aaagaaggtc tgtacacata actcctgtag
    10441 ttgccaaagc atctgctgga gcagtagaat atatgccagt ttgcaaggta accaatatag
    10501 ttaatacaat aaaaagactc aaagaagaag ggctgtggat tgctgcagct gatatggatg
    10561 gagaaacttt ttataaacaa aatcttactg gtcctcttgg tgtagtaatt ggaagtgaag
    10621 gttttggaat atctagattg gttaaacaaa actgtgactt tatagtaaaa atgccaatga
    10681 taggtaatgt tacatcttta aatgcatctg tagcaggtgg aatactttta tatgagatat
    10741 tcagacaaag actggataaa agtaaatagg catgaaaaaa aatataaatc attacttaat
    10801 aatagatggc tacaatatta taaatgcgtg ggacaatttg aaagaactgg ctaaagagga
    10861 cttagaagat tctcgagaaa agcttattga tgacattata gaattttctg agtttatggg
    10921 ttataagact ataatagttt ttgatgctta taatgttaaa aattctcgag aaaaagttga
    10981 aaaaagaaaa cacataacaa tagtttatac aagagaacac caaacagcag atagttatat
    11041 agagaaattt ataacttctt tatcaaagta tgatgatgta aaagtagcca ctaatgacta
    11101 tgcagaacag caaatgattt taggaaaagg tgctactaga atgtctgcaa gagaattaaa
    11161 gttagagtta gatcgttcca agaataaaat gaaggaaaaa aatatcggtt tgaggaaaaa
    11221 aattcagcga aattggttgg aggaaaggtt agataaagaa acattgtcga aacttgagaa
    11281 cattcgtaga aagcgttgaa attactaggt tctttagact ataatatacc tataaatttg
    11341 ttttatatat gggggagatt tacatgttgg tagcaaaaga aaaaagttat gagttagtgg
    11401 acaattgtca gcaggatgag tataacattg tattaagagc aagtgaaggg gataaaatag
    11461 cactagagta cattattact aaatacagaa attttgtaaa ggcaaaagca aagtcatatt
    11521 ttttaattgg agcagataaa gaagatataa tacaagaagg aatgataggc ctttataagg
    11581 cagttagaga ttttgatgga agtaagacca attcatttaa atgttttgcg gagatatgta
    11641 taacaagaca aataataact gctataaaaa ctgcaacaag acaaaaacat atacctttaa
    11701 attcatatgt ttctttgaat aaaccaatat atgatgaaga gtcagacaga acacttttag
    11761 atattatagc tacaagtata gtaacagatc cagaagagct tataattagt aaagaagagt
    11821 taaaaaatat agaatcaaaa atgaacgaat tgttaagtga cttggaatta gaagtactag
    11881 aattatattt aaatggaaag tcataccagt ttatagcgga caagcttaaa agagatgtaa
    11941 agtcaataga taacgcttta caaagggtta agagaaaatt agaaaaacat cttgaaaaca
    12001 gaaatgatta atagtaaaat acttaccata agcttattgt gagcaaaatt aatttatatc
    12061 gaaagataag ggaggaaatt caaaatggct aaagctaaat acgaaagaac aaaacctcat
    12121 gttaatatag ggacaatagg acacgtagac cacggtaaaa ctacattaac agcagcaata
    12181 acaaaaacat tatatgacag atatcaatta ggagaagcag tagatttcgc aaacatagat
    12241 aaagctccag aagaaagaga aagaggaatc acaatatcaa cagcacacgt tgagtatgaa
    12301 acaccaaata gacactatgc acacgttgac tgcccaggac atgctgacta cgttaagaac
    12361 atgataacag gagcagcaca aatggacgga gcaatattag tttgttcagc aacagatgga
    12421 ccaatgccac aaacaagaga gcatatacta ttatcaagac aagttggagt accatatata
    12481 gtagtattct taaacaaatg tgacatggta gatgatgaag agttattaga gttagtagag
    12541 atggaagtaa gagatttatt aacagaatat gatttcccag gagatgacac tccaatagta
    12601 agaggttcag cattaatggc attagaagat ccaaagagtg agtggggaga taagatagta
    12661 gaattattcg agcaaataga tgagtatata ccagctccag agagagatac agataaacca
    12721 ttcttaatgc cagtagagga cgtattctca atcacaggaa gaggaacagt tgcaacagga
    12781 agagtggaaa gaggagtact
//
LOCUS       DAEMGG010000112        12751 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEMGG010000112 DAEMGG010000000
VERSION     DAEMGG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.113"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            113..514
                     /locus_tag="KPK24_002940"
     CDS             113..514
                     /locus_tag="KPK24_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300592.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQIGK"
     gene            557..850
                     /locus_tag="KPK24_002941"
     CDS             557..850
                     /locus_tag="KPK24_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG2300593.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            865..1305
                     /locus_tag="KPK24_002942"
     CDS             865..1305
                     /locus_tag="KPK24_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG2300594.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(1397..1870)
                     /locus_tag="KPK24_002943"
     CDS             complement(1397..1870)
                     /locus_tag="KPK24_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2300595.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(1870..2658)
                     /locus_tag="KPK24_002944"
     CDS             complement(1870..2658)
                     /locus_tag="KPK24_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2300596.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            2986..4230
                     /locus_tag="KPK24_002945"
     CDS             2986..4230
                     /locus_tag="KPK24_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG2300597.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            4553..5650
                     /locus_tag="KPK24_002946"
     CDS             4553..5650
                     /locus_tag="KPK24_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG2300598.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            5641..6339
                     /locus_tag="KPK24_002947"
     CDS             5641..6339
                     /locus_tag="KPK24_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2300599.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            6342..7406
                     /locus_tag="KPK24_002948"
     CDS             6342..7406
                     /locus_tag="KPK24_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG2300600.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITSMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            7589..8293
                     /locus_tag="KPK24_002949"
     CDS             7589..8293
                     /locus_tag="KPK24_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300601.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            8286..9608
                     /locus_tag="KPK24_002950"
     CDS             8286..9608
                     /locus_tag="KPK24_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2300602.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            9795..11168
                     /locus_tag="KPK24_002951"
     CDS             9795..11168
                     /locus_tag="KPK24_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300603.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEAFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(11540..12478)
                     /gene="pdaA"
                     /locus_tag="KPK24_002952"
     CDS             complement(11540..12478)
                     /gene="pdaA"
                     /locus_tag="KPK24_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG2300604.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         4783 a         1485 c         2141 g         4342 t
ORIGIN      
        1 agctaaaatt attcctttta tttttgtttt ttgtatctat ttggtataat agtattagag
       61 cgtataataa tttaggcaaa attttttaag gaagactaga ggagaatata aaatgaaaaa
      121 atgtctttac tgtaataggg atataaagtc accaaataat aaattagcta taaaatatga
      181 tgacaatatg aatatatatc catgtcgtgc agaatgtaag gaaaaaattg aagaatatat
      241 agcaaaaaaa cctacatata agtttataaa tatattagag gttcttttag gtgtgggcgg
      301 aatattctgc tttttgagta aatggactat tgcagcccaa gttgtacttg gaattgatgc
      361 actggttata tttttattcc cattggcagg atttaagatg aatggaaagt taagtatgga
      421 acaaactaag aatcaaagta ggtctattgc aatatcaatt ttggcattta tagtaataat
      481 aactgtatta tattttaaac aaattggtaa gtagagcata attgtataag acatcattag
      541 agtatgaggt gagtttatgg aagaaagaaa attagcacca aacaatgaga gggaagctct
      601 cataaaaaga ttaaaaagaa tagaaggtca agttaagggt atacaaaaga tggtggaaca
      661 agaacgttat tgtgttgata tattggttca aatatctgct ataaggtctg ctataaatag
      721 agtaggcaca attatcttag agaatcatat aaaaggatgt gtagcagaaa gcattaaaca
      781 tgatgatgct gataaaactg aagagatgat aaaagagctt atggatacta taaataaatt
      841 tacaaaataa ggaggcggtt agttatgaat caacaaggat ttattatgga aattgttgat
      901 gatagaactg caaagttaaa aatgcaaaga cattcagctt gtgctgcttg tggaaaatgt
      961 gctactacaa attcagcaga gtcaaaagaa atattagtag aagtagacaa cacaattggc
     1021 gcaaaagttg gtgaccatgt agaagtgagt atggataata tgaatgtatt aaaagcagct
     1081 gtaatggctt atataatacc tcttatagca cttcttgtag gaacaatagg gacttattat
     1141 atattggggg caattggaat gacaactgga gttgaagcca taagtggatt agttggaatt
     1201 tgtcttacaa taatctgtta tttatactta aagaagaatg acaaaaaatt tagagacagt
     1261 agagaattta taccagtaat aacaagaata ttaattgacc tttaagataa ttataattaa
     1321 aaaatagcaa tgtgcttctt aaataaatac tcaatacgta tttgtagagt ttaagtacat
     1381 tgctattttt atatttttat tttgattttc tgtaaggaat aaagtttttt ctgattttca
     1441 aaagattcaa tgttcttaca aaagaagtta caactaatac accaagtgct attgcagcac
     1501 ttagaaatag cgcatctgta ccttttgaca tagccaaaga ataatttttc tgaatcaaag
     1561 acaacattgt attatatagc ccaattcctg gtattagagg aagaatacct ggaattacga
     1621 acacaatagc tggttgcttt agttttcttg ctaaagtctc actgcacgct gagaccaaag
     1681 cagcagcgat aaaacctgca aaaactgcat ttgtagtaaa attaaataaa taaacatata
     1741 caatccaacc aattccgcca gttactccag ctggaagaag tagatatagt ggagcattaa
     1801 agaaaacagc aaatccagca gttgcaacac ctgaaaataa aaaatgcaaa atcatagaca
     1861 tatcagtcat tagaaaggac ctcctattct ataccatgta tcaaggacaa aaccaactcc
     1921 agaagcaatt gcaataataa tcatagcagc atcaaaagca cgagaaattc ctgatattaa
     1981 attccctgat attaaatctc taatagcttt tataaaagca actcctggga gtagtggcat
     2041 tatagcgccg actataagca ttttagggtc atctaaaatg cctatttcaa ctagtagaac
     2101 tcctgaaagt gcaattaaaa aagaagatac taaacaggaa aatgcagata ttgcacttat
     2161 cttcatcgtt ttatcataag taattgcagc aaagattgct attataaacg taagtataaa
     2221 atttaataca taattccctc caagcaaaca agcaaaacaa gcagatgcaa ttcctgtaca
     2281 agagtaaaat aaccataaag gataaggttt tacatcttgg atttcataaa gacgtgcaat
     2341 ggcattatca atatcatact ctttgtcact aacaaattct cgagaaaaac tgtttaataa
     2401 agaaatctta tttaagttta tacctcttga ttgaatcgtt ttcataaacg aaaagccatc
     2461 aaatttctca tctgaaataa ttattactgt aggagttgta aaaatattta catgataaaa
     2521 ccctcttgac ctgcatattc taaggacact gtcttcaacc ctagatgttt cagctccatt
     2581 ttttatcatc agttccccta caaatagagc tagttttaga atatttttct tatattcaag
     2641 ttcctcattt tgattcataa atacacctcc aaattaaaaa acgtaatatt gaaattatat
     2701 caaattttta agtaatattc cttaatatct atacaaatat attatctttt ttttgtttca
     2761 aaatagtcac atttaaatta tatccaatta aaagaattag acaatattat ttattatatt
     2821 ttaacttaaa aataaagaaa gtaactatgt taaatatgga aaaatggcaa aacacttgac
     2881 tattaattgg taaaggacta taataataaa ttatcatatt gataaaaacg aaaattatga
     2941 aatgtctgta aaaataaaat ttaaataata actaggagga gtaatatgtt tgagaacttg
     3001 aaaaaagcgg accctgagat ttatgaaagt atgaaaagag agttaaaaag acaacaaaga
     3061 aatattgaat taatagcatc tgaaaacata gtatcagttc cagtaatgga aactatggga
     3121 agtcatctta caaataagta tgcagaaggt tactcaggca aaagatatta tggtggatgt
     3181 gaatacatag atgaagttga aactcttgca atagatagaa tcaaaaaaat atttggagca
     3241 gaacatgcta atgttcaacc tcattctgga gcaaatgcaa atattggtgt atattttgct
     3301 atgttaaaac caggtgatgt tgttatggga atgaatttat ctcaaggtgg acatttaact
     3361 catggtgctc cagttaatat ttctggtcaa tattataaat tttatgaata tggtatagat
     3421 aaagacagtg gacttataga ttttgatgaa gttagaaaga tagcacatga agtaaaacct
     3481 aaaatgatag ttgcaggagc aagtgcatat cctagagaaa tagattttaa aaagtttaga
     3541 gagatagctg atgaggttgg agcattgctt atggtagata tggctcatat agcaggtctt
     3601 gtggctgctg gtcttcacca aaatccatgt gaagtggctg attttgttac aacaacaact
     3661 cacaaaactt taagaggacc tcgtgggggt gttattcttt gtaaagaaaa atatgctaaa
     3721 gacatagaca aagcaatatt ccctggtatt caaggtggac ctcttgaaca tataattgct
     3781 tctaaggcag tttgctttaa agaagcttta agtgatgagt ttaaagaata tcaggtacaa
     3841 gttgctaaaa atgcaaaagc cttagctgaa gaacttatta aaagagattt taaattgatt
     3901 tctggaggta cagataatca tttaatacta ttagatttga ctaataaaaa tataactggt
     3961 aaagcagcag aaaagagatt ggatgatgct tatataacag ctaataaaaa tacaattcca
     4021 tttgacccaa atggtgctct tgttacaagt ggaataagac ttggtactcc agctgtaact
     4081 actagaggta tgaaagaaga agatatggca ataatcgctg aggctataga cctatgttta
     4141 acttatgatg aagaatctaa ggctagaaca ttggttgttg gtttaactga aaaatatcct
     4201 ttatatgaag aatataatat tatggattaa ttataaagaa caactaaata aaatacattt
     4261 aaataattac aatgattgtg tatgtatttg aatatattta gatataaaaa aggtgtctca
     4321 aaatgaactt tttagtttat gaggcacttt tttgtatgga atcgaacata tttgtattat
     4381 ttaaataata aaaaatatgg ttatctctat ttttagatgg tctctttgta ttttttaatt
     4441 aaaactttaa gatttcttaa gaattatact aggtttttct aaagatttaa agtctatact
     4501 tataagtata gtaatcatat gaaaagaaaa gctttaacga ggagggaatc ttatgaaggt
     4561 attaattctt acaggaaaat ttggtatggg tcattactca gcatcaaatt ctttaagtga
     4621 agatataaaa gcaaaatttg ataattcaga aataataatt aaagatatat ttgagtacat
     4681 tatgcctaac tattcagata aaatgtacaa gactttttct attcttgtaa atcggggaag
     4741 tagtctttat aatttgtttt ataaatgtgc tgaaaatgga aagaaagata taaaatttac
     4801 tttttcagat tattttttaa ataaattaga tacattactt catgaagtac aaccaacagt
     4861 agttatttca acttttccat tttgttctca attagtatca agatataagg aaaaatataa
     4921 ctcaaatctt cctttaataa catgtataac tgatataagt agtcactcag aatggattag
     4981 taaaaataca gactgttatt tagtagctag taaatctact aaggaagaac ttgtttttaa
     5041 aggaatagat gaatctaaga ttaaagttaa tggtattcca gtaaaaaaag aatttaaaag
     5101 aatagaacat gtaaatcatt caactaaaaa aaatattctt ataatgggtg gaggattagg
     5161 attactgcct aaatcagaac aattttacaa agaattaaat agtttagaag gagtaaaaac
     5221 tactgtaata actggtaata ataaaaaaat gtactataaa ctatatggta gatatgaaaa
     5281 tatagaggta gtaggttaca ccaatgaagt atataaatat atgaaagatt ctgaccttat
     5341 tatttctaag ccaggtggaa taacactatt tgaaacaatt tattcagaac ttccaatatt
     5401 ggcatttaat ccatttttac aacaagagat agataatgct agttttatat taaataatga
     5461 aattgggaga atacttggta aaaataaaaa atactatgta gatgagataa aagatttaat
     5521 ttatgatgat gctactctta aagaaatgag cagtaacatg aaagaattaa aaaaacaatt
     5581 tgataataat accttagaga atattttatt ttcacttgat gaacaaggag cgtgtagaga
     5641 atgtatgtag taggtttaat tatcgttgtt gcattgattt ttttagtcca ttcaattata
     5701 cctacatact ataataaact attaaataaa gaggtattaa aaaatatggc tggagaaaat
     5761 gaaattgcac ttacatttga tgatggtcca gataagagat atacagaaaa attactagat
     5821 gtgttaaaag aaaatgatat acaagctatg ttttttgtag tagcaaagaa tgctgaaaaa
     5881 gagcctgaaa ttataaaaag aatgttgaga gaaaatcata tagttggttt acattcattg
     5941 gaacacagaa atgcatggct ttatagttat agttatgtta aaaaagattt tattgaaagt
     6001 acaaatataa tgaaaaattt aggtgtagat gtaaactact atcgtccacc atggggtcat
     6061 actaatatat tttcaaattc atttgttaaa aaatataatc taaaaatgac tttgtgggat
     6121 gtaatggcag aagattggga gaaagatagt acagtagaca ttattataaa caagttaatg
     6181 agtagaacaa aagaaaattc tataatttgt ttacatgatg caggtgaaaa ttcaggtggt
     6241 gcagtaggtg cacctgagcg tactattgaa gctttaaaga tagctattcc aaaacttaaa
     6301 gccagtggtt taaagttcgt aacaccagaa aggatgtagt tatgataagt aaaactgaaa
     6361 agaaaaataa atgcctaggt agtgtagctt ttttagtttt actaatgggc ataacaggat
     6421 attttgtttt tagagggcag tcagttgaat cattgataaa atcattaaaa ggtgcaagcc
     6481 ctatgtttat actaattgga tttgcaatga tgtttatata tgttgcctgt gaaggtataa
     6541 atatatactt aggtatgaaa gctttaaacc aaaagactac tcttttaaaa tgtatgggat
     6601 atgcttttat tggattttat tttagttcta ttacgccttc agcatcaggt ggtcaaccag
     6661 cacaagttta ttatatgaaa aaagacgata taaatatatc atattcttca cttatactat
     6721 tagtcattgt tgttattcac caagtagtta tactggcata tagtggaata atgttcatta
     6781 tggaacgtga gtttatatta aataatgtaa gtggtatgaa cattttacta atatatggtg
     6841 taataacaaa tgttgctctt gtcattggag taattgcaat tatattttca aaaaaacttg
     6901 taaacaattt tataatatca ataacaaatt tattaggtaa gttaagaata ataaaagatg
     6961 ttgaaagttc aagaaaagtt ataatatctc aaattgaaga gtatgttaaa ggtgctcaat
     7021 atataaagca gaatccaaaa cttgtagtcc aaattttagt cataactatt gtacaaataa
     7081 catctatgtt tttagttcct ttctttgtat acaaagcttt tcatctatca acatatactg
     7141 tatttgagat attagcgata caatccttgc ttaatattgc tgtttcttca ttaccattgc
     7201 caggggctgt tggagcttct gaaaatagtt ttatgacatt atttaagata tttttccctg
     7261 gtcacttatt agttccggca atgttactta gtaggggaat aagtttttat gcatttgtag
     7321 ctataagtgg attgatatgt atagtagtac atgtaaaatc ttctgaaaaa gtaaaagcag
     7381 tgaaaaaagt tgcttatgta agataatatt atatagatta tttttattaa tatgtcaatt
     7441 tgtgtgtata ttttaattaa gctttaaaca tgtttacagg agtagaaact attaaaacgt
     7501 gtgacattgc taagcttttg ctaataaagt ataatatttt atatagtttg aaatatttaa
     7561 gaaatatagc aaaaaaagga gcttggtgat gaataaagag attccaaaaa aaattttagt
     7621 agctgatgat aatcctgaga ttagggaaat cgtagaaata cttttaactg gtgaaggtta
     7681 tgaagttatt atggcaacga atggagaaga agctgtaaac ttagtagata gtacgattga
     7741 tttgattatt ttggatgttg taatgcctgt aaaaacagga tttgttgcat gtaatgaaat
     7801 tagagaaaaa acgacagcac caatattatt tttaactgca aaaactcaag actctgataa
     7861 agttattgga ttttcagcag gaggagatga ctatttatca aagccttttt catattctga
     7921 actaatttca agggttaagt cgctacttag acgttattat atataccagg gaaaggaaaa
     7981 ggaaagagag ccaaatatat taaaaacaaa agagttatgt gtcaatttag atactcaaga
     8041 tgttacacta aatggagaac ctgtgttttt gacaactata gaatatagta tattaattct
     8101 tatgttgaaa aatagaaaaa aagttttttc gtctgaaaat ttatatgaaa ctatttggga
     8161 tgagcagtat ttttatacag caaataatac aataatggtt catattagaa atttaagaaa
     8221 aaagttagaa aaagaccaaa aaaatccaca atatataaaa accgcatggg gaaaggggta
     8281 ttatattgat taacaaaatt aagccatcta agttgggagt tatactattg atatatgtag
     8341 taatctcagc tgcagtggct ttcattttaa atataccatt ttactctgct gttgaatcta
     8401 tagtaaaaga tgtatcagaa gggaaagtat attttaaaag agaagtaaat caacttatgg
     8461 ataattttca aacttatgtg aataacaatg gtataagagc agatgatgga gatgatatag
     8521 caaaatggaa tagtgataat tggtttgttt ctatgaagat atataaagga aataaggttt
     8581 tttatgatac attatactat cctaatgaag tacctgataa gagtgaggaa aatgagtttt
     8641 atagcagtcc catggctaga aactatgaga tacaattttc tgatattaaa gctaatatag
     8701 ttgtagtagc tttatttaga tttagatttc aaaattttat agaagcaatt tctattacat
     8761 ttatggcact tacatttatt ataacatttt tactgttgtt tagtagaaag ataaagtacc
     8821 taaggaaaat agaaatgggt ataaagatag tagagagtgg cagtttaaag tatagaatcc
     8881 cagtaaaagg taatgatgaa ttatcatcac ttgcatctag tattaatgag atgagtcata
     8941 cattggagaa tcaacttata aaagaaaggg agatgaagaa taaaaataaa gatatagtaa
     9001 cttcgatatc acatgatgtt agaactccat taacgtcagt aatttgttat ttagatttga
     9061 ttaaagacca taaatacaat agtcctgagc aattggaaca atacataaaa aatgtaaggt
     9121 ctaaagcata tcaaataaaa gatttaacag atgacctttt tacgcatttt gttaattctg
     9181 gagaggaagt ttcaaaaggg tatgaaaaaa taaatgctaa tgaatttatt tttcaattac
     9241 ttttagatag ttcatataca ttggaggaga aaggatttca tataacaata gaaaatgaac
     9301 tttataacca atatcatatt tatgtagatg ttacacaatt tagaaggata tttgataatt
     9361 tatgttctaa tataataaaa tatgctagga aagataagcc tatagtattt ttaattaaag
     9421 tagaggattc taaattacta attatacaaa aaaatagagt aagtaatttt tctaagaatg
     9481 tagaaagttt tggaattggt ataaagaatt gtgagaaaat agttgcatat cataatggaa
     9541 gcataaatgt aaattcagat gataaagaat ttgctattga aatatctatt ccagcaaaag
     9601 aatcataaaa atttaaatta tatattgtgt aattgctatt atatattgtg taattgctta
     9661 tgtaaaattc tatgaagcag gaagcatgtg actttagtta tgggatgttc aatattgtgt
     9721 acctctaggc aagtaacaag tttacttaca agaccatata atattattat cttaaaaaaa
     9781 ggggacataa taatatggtt ttgacaggag cacattatat ttacattcta tttatggtaa
     9841 ttatattaat tactatgatt atgaaaaagg atacaatagt accatgtata ttaggtgttt
     9901 tttttatggg attgttcttt gaaaaaaaca tctttggtgc tataacagca gtgtttaact
     9961 cttttattat atctttaaat gagttgggtc caattatctt gattatagct attatggttg
    10021 cattatctaa agccctagaa gccaataatg ctatacagta tatggtgaga ccattttcga
    10081 gagttataaa aaattctaat acagcatttt ttgtaactgg atttgttatg ttagttcttt
    10141 cttggttttt ctggccaact ccagctgttg ccttagttgg agctgtattt ttacctgttg
    10201 ctatgagagc tgggctacca gcaattggtg ttgcagtagc attaaattta tttggtcatg
    10261 gtcttgcctt atctacagac tttgttattc aaggagctcc aagtataaca gctggtgcag
    10321 caggtgttgc agtatcatat gttataaatg atggtatgat tttgttttgg gtgatgggaa
    10381 tcgtaaccat atctatggca ttttatacat taaaaagaga tattaacaaa ggaatgttta
    10441 gagaagaact aaaaggtttt gaaagtgaag aagtagaagc gttcaatgga aaatctaaaa
    10501 tagcaactat tttagtagct ttgggatttc ttgctgatat aattgctatg tatgtatttg
    10561 acctaaaagg aggagatgca tcagctcttt tgggtggaac agcagtattt ttaataataa
    10621 ttataaatgt tataaatttt ggcaaagata gtcttgaaaa tgtatgtgag aatataattg
    10681 atggttttgt gtttgggata aaaatatttg gagcaattat accaatagca gcatttttct
    10741 atatgggtga agttgcacca ttgacaggtg tatttggaaa agtattagca ccaggttcac
    10801 aaggtttatt atcagatata ggcatagctc tttctcaaac aataccactt aataaatttg
    10861 ccgtatcagg tatagaaact gtagtgggag caataactgg gcttgatggt tctggatttt
    10921 ctgggatttc attagttggt tcacttgcct ctgtatttgg aactgctata aatgcaagtg
    10981 ttggtgcatt agctgcatta ggacaaatca gtggtatatg ggtaggtgga ggctgtctag
    11041 ttccttgggg tcttatttca gcagcggcaa tttgtggggt tagtccaata gaattggcaa
    11101 agcgaaactt tgtcccagtt ataaccggat tggttgtaac aaccatagta gcaatgttta
    11161 ttatttaata aatcgaataa atctttaatt gtataaatta aaaatagatg tgtagctata
    11221 atttattata attgttttac taattctaag ccatacatct atttttattg atatttttat
    11281 tagtattctt taataaagta atatttttca ttatattata cataacatct aaatataaca
    11341 tctaaaatac tgttatctat atttaatatc ttgccaaatt ttttgatgtt ttattgccct
    11401 gtcccaccaa catatataag ctatcacaag tttttaccta tactatcacc aaacaaaaat
    11461 tctaatttta atttattatt ttataaccta tattatatta actatattag tttgtagact
    11521 ttagatgtaa ttataaatat taatactcca agagttcaaa ttcatatcct agttttcttg
    11581 catttgatat aaaatcattt aaaatttctg tactagtttt ggaaactgca tgtagtaaca
    11641 agatagaacc atcatgtaga ttatccatta ttttttgcgt tgcttcttca tgtgatggtt
    11701 gtttatcagt atcccagtct ctgtatgcaa aactccaaaa tacagtcttg tatcctaaat
    11761 ttttagtcat tgccaagctt ttttctgagt attttcccat aggaggtcta aaaaatttaa
    11821 ccatatcttt cccagttaca tctttatata acttttcaac atcatataat tcgtcattaa
    11881 agttttttag attgcttgtt tttgttggca tagatggatg ggttttacta tgatttccaa
    11941 ctatatgtcc ctcagaaacc attcttttta ctaaatcttt attttctttt atatatggag
    12001 ctgtaacaaa aaaaactgct ttaacctggt tttgttttag agtgtcgagt atttttgtag
    12061 tgtatccatt ttcataacct tcatcaaatg taagatatag tgttttttga ccatcttttg
    12121 taggtccatt gtatagggca tcatagtcag aaaatttaaa ttcaatgtcc gtatttactt
    12181 ctggtgtttt atgcttattg tttggtatat aaaaccaatc aagagtagtg ttatctaaag
    12241 aagatacatc taatttatta ttatcattat tagtagtggc ttttgatggt ttatctgttc
    12301 cgtctgaaac tattacattg gaagtatctt taccactatc tattttttcc ttatcttctt
    12361 gtaactgtgt ttccttattt tgattttcgt tttgattgtt ttgtgaattt gaacatccaa
    12421 caataaaaaa aatagaaata gaaagtagtg ttaaataatg caatactttt ctaaacattt
    12481 tgaagctcct ttctaaatta ataataattg catataataa taattatatg attaattaca
    12541 attgcatata gtattattat aattggaaat tactattaat aatagttata tctttaaaaa
    12601 taaagtcaca atagtatgac aaagggtgaa taatcaataa atttaaaaaa aaatgaaaaa
    12661 aatagttgac catattatga gaagatgtta ttataataaa tgtgctcaac aagaagtaac
    12721 aaaataacaa gcacaaagaa gttaattgaa t
//
LOCUS       DAEMGG010000113        12526 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEMGG010000113 DAEMGG010000000
VERSION     DAEMGG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.114"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(348..893)
                     /locus_tag="KPK24_002953"
     CDS             complement(348..893)
                     /locus_tag="KPK24_002953"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2300605.1"
                     /translation="MRVEPIENKKDIEKLKRYFRNKGKIRDLTLITLGLNTGFRIEDI
                     LKLRVKDVYKKREINIKESKTGKRIRKPINDSLKEILDIYCKDKPTNEYLFKSQKGEN
                     EAISTTQAYRIIKKASKACRIKLNIGTHSMRKTHGLHIFKDKGIEVAQRALNHANQID
                     TLTYIGVVQKQADNAIMKLNL"
     gene            complement(909..1157)
                     /locus_tag="KPK24_002954"
     CDS             complement(909..1157)
                     /locus_tag="KPK24_002954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300606.1"
                     /translation="MEGTKRSYRKIPKLDLRKVKLADVFEKNKSGVFRVEEADEQLLH
                     ECLDETKSIREEKHLTWQIQFKGKSVYCVFYEHPFWDL"
     gene            complement(1144..2079)
                     /locus_tag="KPK24_002955"
     CDS             complement(1144..2079)
                     /locus_tag="KPK24_002955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300607.1"
                     /translation="MINIIDLRFYLRNDLFTNEELTEITKQITEENVFLYDIAEEKGC
                     HPYTLNKCLQRFEHSSMEVGKFIGEMSELHKEGALTTEELKLKFEIVKEILILIKRSE
                     EAEKILHLLENEKVKTVAEKLYKSRSSVYDILKQMGFKKNKYINVWYHIDNENIAGNV
                     ENTKRDVELVHLYQQRYDNFLKNYYLDSEIIDIDKNILDDIETLSEEFEYETVNDFIS
                     VLLLRGLQNERFKNKENINNKNDNNNIVKMKDVEHEIFFTQGAEMLEEYYKTNLIDVH
                     KISKTEIEEMNPKELMELYHLKNEGKAKEDFYGRD"
     gene            complement(2250..2897)
                     /locus_tag="KPK24_002956"
     CDS             complement(2250..2897)
                     /locus_tag="KPK24_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2300608.1"
                     /translation="MIIGYERVSTRKQDNSRQERVLENEGIKFDKIYRDKVTGATKER
                     EALNRMLLEVNEGDTVYCESISRLGRNLKDLIDIIDTLVQKGVRVKILKEGIDTGNNT
                     YKLLLGIFGAVAEMERETIQERTQQKIDQLKEDKKNTGEINTKSGKWFGRQKVTKELL
                     ETNKEFIKYYNKILDGSLKKNEVAKILNVGRTTLYEWIKVYEGGEIKRSNRAKKN"
     gene            complement(3487..3855)
                     /locus_tag="KPK24_002957"
     CDS             complement(3487..3855)
                     /locus_tag="KPK24_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2300609.1"
                     /translation="MELDFKFIGQRIKIARIKKKLTQEVLSEKINVSPQHVSNIETGN
                     SSVSLPTLVAIANTLGVSVDELLCDTILSSKAVFENELEEVLRDCNEYEIRFITDIVK
                     ASKKSIRDIKLFQSNLNDRY"
     gene            3971..4303
                     /locus_tag="KPK24_002958"
     CDS             3971..4303
                     /locus_tag="KPK24_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2300610.1"
                     /translation="MDIPKIPQTELKVMKFIWSKNDIVTSKEVAKAMELEYSWKMTTT
                     LTILSRLVIKHFLASERIGRMTHYTILIAKKDYKISETKRFLNDIHSNSLESLLNSLK
                     DCNITHKK"
     gene            complement(4451..4888)
                     /locus_tag="KPK24_002959"
     CDS             complement(4451..4888)
                     /locus_tag="KPK24_002959"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300611.1"
                     /translation="MRVKNIISIFVCALIIIMSVNVVSFAEQFNSSDIIFMEDGKEEI
                     NPFSVYILNGRCNLSFSNGKANVNASVVGKSGVTRTSIVTKLQELKGGSWVNLKTSTK
                     KTGGKTCSVLNSYSVTKGHTYRAVAIITAENESKTLISTSQKY"
     gene            complement(4957..5658)
                     /locus_tag="KPK24_002960"
     CDS             complement(4957..5658)
                     /locus_tag="KPK24_002960"
                     /inference="COORDINATES: protein motif:HMM:NF025643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HBG2300612.1"
                     /translation="MNKDKLDIEKLLYEQLPKIEEKILYELPCDEDIDYEFSIEFESK
                     MDKIIKENEKKVKKNTYKNLKKIAIVFIIILSSIVTITTIKTEAFPENLIRIMKKVYK
                     EFTDYNFINPHKSNVSFKYIEPKYISTGYKEVERIEEENYLSILYKDKLDNYIIYQMM
                     LYSRDVIVLDTEDAFVENIKFNNQYAQYVEKGNTQQLLWHDKNNIYMLSKELPTKKIT
                     KKDKKEFIKIAKNIK"
     gene            complement(5651..6196)
                     /locus_tag="KPK24_002961"
     CDS             complement(5651..6196)
                     /locus_tag="KPK24_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2300613.1"
                     /translation="MLIYLAIIDSKEERIKFEEIYIKYKKLMFYVANQILKDDVLAED
                     AVHSSFLKIIDNLDKVNDVNSPKTKGFVVIIVKRISINIYNRRKKEEISNINDDNYKF
                     SSLDLSIEKLGECSYLGEALKKLSETDLQVILLKYSHGFSIREISKLLNIKEVNLYKR
                     NKRALEKLKKILSEMEALSNE"
     gene            6332..7060
                     /locus_tag="KPK24_002962"
     CDS             6332..7060
                     /locus_tag="KPK24_002962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300614.1"
                     /translation="MKSKRINKMLATTMTMAMLAIPAVTATSYAMESTGLGTEYTYNN
                     ESKQQEEWVKSFDTETGKEILLNKTTNEKVSEVYENVNGKLVKLSINEALEKFNLNEE
                     IITKSPKSIFGVSSVKKITGSARKISQDVSGGKQGATISIGNSTTVTESFTVGGTTEA
                     IKAAVRANAGFTWSKSLTRSVTTTHKVPAGKIGYVAFKPYYKEVRGTVNGILAGTSIT
                     QKIVAKSPLKAGKVCDGLEYLAYK"
     gene            7086..7334
                     /locus_tag="KPK24_002963"
     CDS             7086..7334
                     /locus_tag="KPK24_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300615.1"
                     /translation="MENKNMYLRVSLILAVLGFIILFFNNDLALSLKATYLADKGFED
                     IVENQILKNYSYMFLIIGGVLFSIGIYNLTKLKQINKK"
     gene            complement(7520..10129)
                     /locus_tag="KPK24_002964"
     CDS             complement(7520..10129)
                     /locus_tag="KPK24_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2300616.1"
                     /translation="MKIDIKQLIDIIKNANIDELYLRDIGVDTTQKRNVPCPIHGGKD
                     KNFQFDLNKRIYTCYSHNCVVGADIIELCRTYERFERPIEAILFLANKYNISLPINKS
                     KTNIKKTNNTIKNIYIKKKDINSFIEEKKQEAFKNNDIELAFEFECMTDKDKRKYYLD
                     NNIKIKSMLEKQNYSSYKADSIINIDKYISENLKGIRESIKHATEGKNVLMVAPTGSG
                     KTYSIINTLKELDIKALFIFPNSANVQQAIVEYKIAGAYDKIPTKHALEGNKLVAMTW
                     DKTEQLLKEDLSEYIIVIDEIHQTYTDAFRSKAIKSLNNITNKCRGRLDITATPSKLE
                     FEIYNKVIEYKQNIQTEYKVKLYDKINIGKVIEILNNSNNSMLLKDSISTLNYISKKV
                     NKKSGVVISDTKDTSKLYDRIVSYSDMEGYEVLLNTSVITAGVNINNPNVTDIIVIGE
                     KDVGKIKQYVARARGAKSINVHIFNSYKTTNEDSNVYSVEWCINENIKDVESLTNIYN
                     KASKRDLPYRAIGIDISPINIKNIDSNIYYDKKDMCYKTDKLYIKSNAYNNYYQTRTI
                     NSFKVLLEEYFNKIEIVETEKETKKIEEEIKEHEEAIKEFKKSAIEQLEGHKKYLVGY
                     SEIKKSMTSSNLLKYHHDMRIDNNICLKYYMEHDLYSLIINNNCRKTIDLFSDFVLDN
                     SYSIDLSWKLATMSDEKRNLFFEQINTTIYRKINKKYANSLLNDDNIGTYTYNYINFN
                     FGVGTSYTKEHLEALAVDLQTFLATKKKLTIKKISLILQSIFVIEVKQHRGVTGLPYK
                     YYKNILPNPVTDKKVIRVYTIKKHIDIEDIKKELGLIDNDLSIEHLVEARINKLLNAI
                     DETEKEVLLEGLI"
     gene            complement(10459..10761)
                     /locus_tag="KPK24_002965"
     CDS             complement(10459..10761)
                     /locus_tag="KPK24_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300617.1"
                     /translation="MLKDDLLRIGFDTYGVNLEDKLLENNEIYLNDKKESERIMNRLL
                     EILEEKEHKKMLIDFEEKVFGNSVIEKEYAYRMGVLDGLDLAEFIDLNKKLCLKRN"
     gene            10930..11133
                     /locus_tag="KPK24_002966"
     CDS             10930..11133
                     /locus_tag="KPK24_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2300618.1"
                     /translation="MDIQINKLLENQNKTRYWLAKQIGMTHQNLTKLANNNTNSIKFD
                     SLEKICMALKCSPNELFGWEQDK"
     gene            11213..11758
                     /locus_tag="KPK24_002967"
     CDS             11213..11758
                     /locus_tag="KPK24_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011572951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300619.1"
                     /translation="MDRYTFDNLPIKEQIQYINNKLLEGNTLTNTCKDIGIGRTTIRN
                     RFKQHGYEFNPTKKLYISIIEVVELESSSKVVAGDTSNDNTLVDATNIDFNNILSKFN
                     DMNNKLNEVYSWYESQSSNKVVGADKLNIDDFKGNIVVRSYKVYEDIQKEFVAFCEAN
                     KKYRVQDIISQALRDFMDKYK"
BASE COUNT         4134 a         1982 c         1342 g         5068 t
ORIGIN      
        1 aaaaaaaaat aaccatacgc attgatttta cttaaattaa aagtgttttt cgagttgttt
       61 tttcacttag tttagtaatt ttgaaatatt ataatattaa gcttcaatat gtatctatta
      121 tcatttaata atcaattaat aatagatatt ttttctaaac aagataaaaa caatatctat
      181 accagtaata gcaatagata gaagtatata aaaaaatgta cgattactat aaactattac
      241 atttatttta gttgccttga tacattgcaa ttacttacac atacgcctat ttaattaatc
      301 attaactata aaatgtaaaa acagcaaaga aaatcataca tttttaacta taaattaagc
      361 ttcataatgg cattatcagc ttgtttttgt actaccccaa tataagttaa tgtatctatt
      421 tgattagcat ggtttaaagc tctttgtgct acttcaattc ctttatcctt aaatatatgt
      481 aaaccatgtg ttttcctcat actatgagta cctatattta attttattct acaagctttt
      541 gaagcttttt taataattct atatgcttgt gtagtgctta ttgcttcatt ctctcccttt
      601 tgacttttaa ataaatattc attagttggt ttatctttgc aatatatatc taatatttcc
      661 ttcaaactat catttatagg ctttcttatt cttttccctg tcttactttc ttttatattt
      721 atctctcttt tcttgtatac gtctttaact cttagtttta gaatatcctc tatcctaaag
      781 ccagtattta accctaaagt tattagggtt aaatctctta ttttaccttt atttctaaaa
      841 tatcttttaa gcttctctat atctttttta ttttcaatgg gctctactct catttaaacc
      901 actcctattt ataaatccca aaatggatgt tcataaaaaa cacaatatac acttttccct
      961 ttgaattgta tttgccaagt aagatgcttt tcttctctta tacttttagt ttcatctaaa
     1021 cattcatgta aaagttgttc atcagcttct tccactctaa aaacgcccga tttattcttt
     1081 tcaaatacat ctgctaattt tacttttctt aaatctaatt ttggtatttt tctataactt
     1141 cttttagtcc cttccataaa aatcctcctt agcttttcct tcatttttta aatgatatag
     1201 ttccattaat tcttttggat tcatttcttc aatttctgtt tttgaaattt tatgaacatc
     1261 tattaaattt gttttataat attcctctaa catttcagct ccctgtgtaa aaaatatctc
     1321 atgttcaaca tctttcattt taacaatatt gttgttatcg ttcttattgt taatattttc
     1381 tttattttta aatctttcat tttgaagccc tcttaaaagt aaaaccgata taaaatcatt
     1441 tactgtttca tattcaaatt cttctgacaa agtttctata tcatcaagaa tatttttatc
     1501 tatatcaata atttcactat ctaaataata gttttttaaa aaattatcat atctttgttg
     1561 atacaagtgt actagctcaa catccctttt agtattctca acatttcctg ctatattttc
     1621 attatcaata tgataccaaa cattaatata tttatttttc ttaaatccca tttgttttaa
     1681 aatatcatat acacttgacc ttgatttata cagtttttct gcaactgtct tgaccttctc
     1741 attttccaat agatgtaata ttttttcagc ttcttcagac ctttttataa gtattaatat
     1801 ttcttttaca atttcaaatt ttaatttcaa ttcttctgtt gttaaagctc cttccttatg
     1861 taattcactc atttcaccaa taaatttccc aacctccatt gatgaatgtt caaacctctg
     1921 taagcattta tttaaagtgt atggatgaca gcctttttcc tctgcaatat catataaaaa
     1981 cacattttct tctgttatct gcttagttat ttcagttagt tcttcattag taaataaatc
     2041 atttcttaaa taaaatctta aatcaattat attaatcact cctttttaat tatttttcat
     2101 acattaatct tatcaccaaa aaaattaaat ttaaatattc caagacatca tctggacgct
     2161 aaaaagtgtc gtttttatta atttaataat attatcaaaa aaaagagcaa agacagtccc
     2221 ctaaaataaa aaaagaacta gttataaccc tagttcttct ttgctctatt gcttcttttt
     2281 atttctccac cttcatatac ctttatccac tcgtaaagcg tagttctacc aacatttaat
     2341 attttcgcca cttcattctt ctttaaactt ccatcaagaa ttttattata atattttata
     2401 aattctttgt tggtttctaa aagctccttc gttacttttt gtctaccgaa ccattttcca
     2461 ctctttgtat ttatctcgcc agtatttttc ttatcctctt tcaactggtc tattttctgc
     2521 tgtgttctct cttgtattgt ttccctctcc atctctgcaa cagctccaaa tataccaagt
     2581 aataacttat aagtattgtt tcctgtgtct ataccttctt ttaatatttt tactcttaca
     2641 cctttttgaa ccaatgtatc tataatatca attaaatcct ttaaatttct acctagacga
     2701 cttatagact cacaataaac cgtgtccccc tcatttactt ctaacaacat tctatttaat
     2761 gcttctcttt cttttgtagc tcctgtgact ttatccctat atatcttgtc aaacttaata
     2821 ccctcatttt ctaaaactct ttcttgtctg ctattatctt gttttcttgt acttaccctt
     2881 tcatatccta ttatcataac actacccccg tttatcttgt tataataatt atacaatata
     2941 ttttccgttc ggtcaaacta tattttctat tttcacaata ccgaacgtat tttattgttg
     3001 atttccaatg attctatgta aaaataacat aaatatcata cgtacggtta aacgttggtt
     3061 tttccgaaca cgaacatatc acctttttta tatcaactat tccttaaata ttttatataa
     3121 aatcgccaaa atctttttat attttttagg caaccaagtt tttaaaacaa aaatggtata
     3181 tcagtaagaa ttatgttttc acacaatata attcttaatg atataccatt taagaacctc
     3241 ataagaggta gatagagata attacataat catcacatac gttttatcaa atctgttttg
     3301 taatagggtt tatacttaaa taataacaaa aataccgtat tttttctaca tttatacttt
     3361 ttttgttgat agtaaaatat ggaaattagt catattttca gttattaact cataagttta
     3421 tacaactttt atttaaatat aataaaaaca tcaataataa ttaaattatt gaagcatata
     3481 tttatattaa tatctatcat ttaaatttga ttgaaacagt tttatatctc taatggattt
     3541 tttagaagct tttacaatat cagttataaa tcttatttca tactcattac aatccctcaa
     3601 aacttcttct aactcatttt caaagacagc tttagaactt aagattgtat cacataataa
     3661 ttcatcaacc gacaccccaa gagtatttgc aatagcaacg agcgttggca aacttacact
     3721 tgaatttccc gtttcaatat tgctgacatg ttgtggacta acattgattt tttctgataa
     3781 aacttcttgt gttagctttt tcttgattct tgcaattttt atcctttgac ctataaattt
     3841 gaaatcaagc tccatatgcg tattcccttc ctacaagtat tcacacatat tgtaaccttt
     3901 cccaaagtat attataataa attaatagaa tgtttcatta tgatagttat agtgtataga
     3961 aaggaattat atggatatac caaagatacc tcaaacagaa ctcaaagtca tgaaatttat
     4021 ttggagcaaa aacgacattg taacatctaa agaagttgca aaagctatgg aacttgaata
     4081 tagttggaaa atgacaacaa ccctaacaat cttatcaagg cttgttatta aacacttttt
     4141 agcatctgaa cgaataggca gaatgacaca ttatacgata ctcatagcaa aaaaagatta
     4201 taaaatatct gaaacaaaaa gatttctaaa tgacatacat tcaaattcat tggaaagcct
     4261 tttaaactct ttgaaagatt gcaatattac acataaaaaa tagtacatga gtataaatta
     4321 tattattata cctatgtact atttaagttt ttctataatg ttcctatatc tatctaattt
     4381 tgattgattt agatacttct gtagtagttt tcaaaacgtt tcttaactct tgtaagtgta
     4441 ttttcttttt ttaatatttt tgagatgtac ttattaatgt ttttgactca ttttcagcag
     4501 taattattgc aacagctcta taagtatgtc cttttgtcac agaatatgag tttaaaacag
     4561 aacaagtttt tcctccagtt ttctttgtag atgtttttaa atttacccaa cttccgcctt
     4621 ttaattcttg taacttagtt acaatagatg ttctagttac tccagatttt ccaacaacag
     4681 aagcatttac atttgctttt ccattagaaa atgataaatt acatctacca tttaatatgt
     4741 aaactgaaaa aggatttatt tcttctttac catcttccat aaaaataata tcagatgaat
     4801 taaactgttc agcaaaacta acaacattta ctgacattat tattatcaac gcacatacaa
     4861 atattgagat tatatttttt actctcatta agtacctcct tataaaatct tatatattca
     4921 atataacaac taactagcac tattttagac aaaaatttac ttaatatttt ttgcaatttt
     4981 aataaattct tttttatctt tcttagttat tttttttgta ggtagctctt tagataacat
     5041 ataaatatta tttttatcat gccacaataa ttgttgagta tttccttttt caacatactg
     5101 tgcatactga ttattaaatt ttatattctc aacaaaagca tcctctgtat ccaagactat
     5161 aacatcccta gaatataaca tcatttgata aattatatag ttatctaatt tatctttata
     5221 taatattgat agataatttt cttcctcaat tctctcaact tctttatatc cagtagaaat
     5281 gtatttaggt tctatatatt taaatgatac atttgattta tgagggttta taaaattata
     5341 gtctgtaaat tctttatata ccttcttcat tattctaatt aaattttctg gaaaagcttc
     5401 tgtttttata gtagttatag tgactatact tgataaaatt attataaaaa caatagcaat
     5461 tttttttaag tttttatagg tgttcttttt tacttttttc tcattttctt ttataatttt
     5521 atccatttta ctttcaaatt ctatagaaaa ttcatagtca atatcttcat cacaaggtaa
     5581 ttcatacagt attttttctt ctatttttgg aagctgttcg tataataatt tttctatgtc
     5641 taatttatct ttattcattt gataatgcct ccatttcgct taaaattttt tttaattttt
     5701 ctaatgctct tttatttctc ttatacaaat taacctcttt tatattcagt agtttagaaa
     5761 tttctctgat agaaaaacca tgactatatt ttaagagaat cacctgtaaa tctgtctctg
     5821 acagtttttt taaagcttct cctaaataac tacactcacc taacttttca atcgataaat
     5881 ctaaagagct gaatttatag ttatcatcat taatattact tatttcctct ttttttcttc
     5941 tattatatat atttattgaa atccttttta ctataataac aacaaaacct ttagtttttg
     6001 gactattcac atcatttact ttatcaagat tatcaattat ttttaaaaat gaactatgaa
     6061 cagcatcctc cgctaaaaca tcatctttta aaatttgatt agctacataa aacattaatt
     6121 tcttatattt aatatatatt tcttcaaatt taattctttc ttcttttgaa tctataatag
     6181 ctaaatatat taacataaat tattctccct taaaaaatat tatccttaaa aaaattatat
     6241 acaatttata aaaaattata tataaatgtc taaaaataaa aacttcattt gttatatata
     6301 taaataaaaa cataaaagga tggtgtttta tatgaaatca aaaagaataa acaaaatgtt
     6361 agcaactaca atgacaatgg caatgctagc aataccagca gtaacagcaa catcatatgc
     6421 aatggaatca acagggttag gaacagaata tacatataat aatgaatcta aacagcaaga
     6481 agagtgggtt aaatcattcg atactgaaac aggaaaagaa atattattaa ataaaactac
     6541 taacgaaaaa gtttctgaag tatatgaaaa tgtaaatgga aaattggtta aattaagtat
     6601 taatgaagca ctagaaaaat ttaatttaaa tgaggaaatt atcacaaaat ctcctaagtc
     6661 tatttttgga gtatcaagtg ttaaaaaaat tactggttct gcacgcaaaa tatcacaaga
     6721 cgtatcaggt ggaaaacaag gtgctactat atctatagga aattcaacaa ctgtaactga
     6781 aagttttact gttggtggaa ctacagaagc aataaaggca gcagtaagag caaatgcagg
     6841 atttacatgg agcaaatctt taacaagaag tgtcacaact acacacaaag tacctgcagg
     6901 taaaatagga tatgtagcat ttaaaccata ttataaagaa gtaagaggta cagtaaatgg
     6961 gatacttgca ggaacttcta taactcaaaa aatagtagca aaaagtcctc ttaaagcagg
     7021 aaaagtttgt gatgggttag aatatcttgc atacaaataa taaataatag aaagagttga
     7081 ttaaaatgga aaataaaaat atgtacttaa gggttagttt aatattagca gtattaggct
     7141 ttataatatt attttttaat aatgacttag cattatcact aaaagcaaca tacttagctg
     7201 ataaaggctt cgaagacata gttgaaaatc aaatattaaa aaactattcc tatatgtttt
     7261 taataatagg tggtgtacta ttttccatag gaatctataa cttaaccaaa ctgaaacaaa
     7321 ttaataaaaa ataaataaaa taagaggact tataaattat aagtcctttt attttacact
     7381 tataagctac tttcaacctt tcatgattta acctttattt atacaacacc caaaactcga
     7441 cctcaaaaag agaacaggac ttcgtccccc ttaatacaaa gcaacatttt ttagtactat
     7501 tcgtacttaa gattagctat tagattaatc cttccaaaag aacctctttt tccgtttcat
     7561 caatcgcatt taaaagttta tttattctag cttcaactaa atgctctatg cttaaatcat
     7621 tgtctattag tcctaattct ttttttatgt cctctatgtc tatatgtttc ttaatagtgt
     7681 aaactctaat tactttttta tctgtcacag ggtttggaag tatattttta tagtatttat
     7741 agggcaaccc tgtgacaccc ctgtgttgtt tgacttcaat aacaaaaatt gattgtaata
     7801 ttagacttat tttcttaata gttagctttt tcttagttgc taaaaacgtc tgtaaatcaa
     7861 cggctagagc ttctaagtgt tcttttgtat aacttgtacc aactccaaaa ttgaagttta
     7921 tataattgta agtataagta ccaatattat catcatttag caaagaattt gcgtattttt
     7981 tattaatttt tcgataaatt gttgtattta tctgttcaaa aaatagattt cttttctcgt
     8041 ctgacattgt cgcaagtttc caagataggt caatactata actattatct aatacaaaat
     8101 cactaaataa gtctatcgtt tttctacaat tattattaat tattaggcta tataaatcat
     8161 gttccatata atattttaaa cagatattat tatctatcct catgtcatga tgatatttta
     8221 ataagttact gctggtcatt gacttcttaa tttcactata gccaactaga tactttttat
     8281 gtccttctag ttgttctata gctgatttct taaattcctt aatagcttcc tcatgctctt
     8341 ttatttcttc ctctattttt ttagtttctt tttcagtttc tactatctca attttattaa
     8401 aatattcctc taatagcact ttgaagctgt ttattgttct tgtttggtaa tagttattgt
     8461 aagcattact ttttatgtat aacttgtccg ttttatagca catatctttt ttatcatagt
     8521 atatattact atcaatgttt tttatgttta tagggcttat atcaattcct atagctctat
     8581 atggtaagtc tctcttagaa gctttattgt atatatttgt taggctctca acgtctttta
     8641 tgttctcatt tatgcaccat tcaaccgaat atacattgct atcctcattt gtcgttttgt
     8701 aactattaaa aatatgtaca tttatacttt ttgcccctct agctcttgca acatactgtt
     8761 ttatttttcc tacatccttt tctccaatta caataatatc tgttacatta ggattattta
     8821 tgtttacacc tgccgtgatt acagacgtat ttaaaagcac ctcatagcct tccatatcgg
     8881 agtaactaac aattctatca tatagtttgc tagtgtcttt agtatctgaa ataacaactc
     8941 cactcttttt attaactttc tttgatatat agttaagtgt tgatatacta tcttttaaaa
     9001 gcattgaatt atttgaatta tttagtatct ctattacttt cccaatattg attttatcgt
     9061 ataacttaac tttgtattcc gtttgtatat tttgtttata ttcaataact ttgttatata
     9121 tctcaaattc taatttagag ggtgttgcag ttatatctag tctacctctg cacttattag
     9181 ttatattatt taagctcttt attgctttgc ttctgaacgc atctgtgtaa gtctgatgta
     9241 tttcatcaat aactattatg tactcgctca aatcctcttt tagaagctgt tcagtcttat
     9301 cccacgtcat agccactaac ttgtttccct ctaaagcgtg tttagtaggt atcttatcat
     9361 atgcaccagc tattttatat tcgactattg cttgttgcac attcgcagag ttggggaata
     9421 taaagagtgc ttttatatcc agctctttta atgtatttat aattgagtat gtttttccac
     9481 ttcctgtagg agctaccatt aatacgttct taccttctgt agcatgttta attgactctc
     9541 taataccttt tagattttca cttatgtatt tatctatatt aattatcgaa tcagctttat
     9601 aacttgaata gttttgtttt tctagcatgg attttatttt aatattgtta tctaagtaat
     9661 actttctttt gtccttatca gtcatacact caaattcaaa agctaactca atatcattgt
     9721 ttttaaaagc ttcttgtttc ttttcttcta tgaagctatt tatatctttt ttctttatgt
     9781 aaatgttttt aatagtatta tttgtttttt taatatttgt cttactcttg ttaattggta
     9841 atgagatatt atatttattg gctaggaata aaatagcttc tataggtctt tcaaagcgtt
     9901 catatgtgcg acataattca attatatcag caccaacaac acaattatgg ctatagcatg
     9961 tatatattct tttgtttaaa tcgaattgga agtttttatc cttaccacca tgaattgggc
    10021 aaggaacatt ccttttttga gttgtatcga ctcctatatc tcttaaatat agttcatcta
    10081 tattggcatt ttttattata tcaattaatt gttttatatc tattttcatt tgtaaaaatc
    10141 cccctttttt agttaggggc taatcaagtt ttatcaagct atgtatttac attctgccgt
    10201 aaatctcgta taattaacat ataaaagaat aaggaaatat atattttata tgttaattaa
    10261 gcctaagtga ttagctccta gtattaaccc ctagtggcta actcctaatc ttgattaacc
    10321 cttaaatgga aagaatgagt ggatgccgtc aaacaaatac tcgttctttt tcgttttttt
    10381 tgttattagt attatatcac tattaaagtt ataattcatt gtttagaatt atttttctga
    10441 cggttttaat atatattttt aatttctttt caagcataat tttttattta agtctataaa
    10501 ttcagctagg tcaagcccat ctaaaacccc cattcggtat gcgtattctt tttcaattac
    10561 actgttacca aatacttttt cctcaaaatc aattagcatt tttttatgtt ctttttcttc
    10621 caatatttct aacaatctgt tcattattct ttcagattct tttttgtcat ttaaatatat
    10681 ttcgttattt tctagtagtt tatcttctaa atttactccg tatgtgtcaa atccaattct
    10741 taataaatca tcttttaaca ttacttttaa cactcctttt tatgcacact taaatctttt
    10801 gcacccctaa atatggtaat atattctatt tattattatg taattattta ctatgtattg
    10861 tcaagcatat atattgtttt tggtacatat ttccgatact atataataaa aaaggtggcg
    10921 attaaattgt tggatataca aataaataaa ttattggaaa atcaaaataa aacacgttat
    10981 tggttagcaa agcaaattgg aatgactcat caaaacttaa ctaaactagc caacaataat
    11041 acaaattcta ttaaatttga ttctttagaa aaaatttgta tggctctaaa atgttctcct
    11101 aacgagctgt tcggttggga gcaagacaaa taaaaaaata taaatcctaa agggtttatt
    11161 tataactact agaaaactac tttagtagtt attttatttt ggaggtttaa acatggatag
    11221 atatacattt gataacttac ctatcaagga gcaaatacaa tatattaata acaagctctt
    11281 agagggcaat acattaacta acacatgtaa agatattggt attggtcgta caactataag
    11341 aaatagattt aaacaacatg gatatgaatt taatccaact aagaagctat atatatctat
    11401 tattgaggtt gtcgaattag aaagtagttc aaaagtagtt gcgggcgaca ctagcaatga
    11461 caatacttta gttgatgcaa ccaatattga ttttaataac attttatcta aattcaatga
    11521 tatgaataat aaactaaatg aagtatatag ttggtacgaa tcacaaagta gtaataaagt
    11581 agttggggct gataaactaa atatagacga ttttaaaggc aatatagttg ttagaagcta
    11641 taaggtctat gaggatattc aaaaagagtt tgtagccttc tgtgaagcta ataagaagta
    11701 cagggtacaa gatattatca gccaagctct tagggatttt atggataaat ataagtagtt
    11761 ttacttactt tctgatattc taaaaacttt tcaatgaaaa tctcattttt tggcttcctg
    11821 tgaaaaatat gtactccttc cagtcctcta tctttattta ctttattaat ccgatagggg
    11881 gcgtattata tatatttcta tcgagcatat actcatcaaa acatagcatt agtttgatta
    11941 ttatatttct atttatttta agattatata tacaatttgc cccttaatac attgcaatta
    12001 ctaacttctt tagttttgat attaatattt aaatcattcc tttttaaagg tgctataagt
    12061 gctatatagg cattgcaacc tatatacttt ttaggggtgc agttgcatat gcaagctctt
    12121 tatttcccaa cacttaacca gttataaata tttatatcct ttattagcca aatatagcat
    12181 tttaaacaac tccgactatc tttagtagta acattttaca tagctttatt aatgtatttt
    12241 tgcatctgca accatgtttt taagttgcaa caccccaccc ccacacctta cacaaaacaa
    12301 tacttatctc cgaagccata tacctacctc ggttcacata tttttcttca acagcgttat
    12361 tttcagcaag gggggctaaa cattatactt tttttcagtg cattaaatag ccttacatgt
    12421 attggtataa ctttatttca aacccttaat tgaattgttt caatctaaat taagtaaatt
    12481 ttaataagat aaaatatata gtttgtaact ttatttcaaa atacag
//
LOCUS       DAEMGG010000114        12492 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEMGG010000114 DAEMGG010000000
VERSION     DAEMGG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.115"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            240..686
                     /locus_tag="KPK24_002968"
     CDS             240..686
                     /locus_tag="KPK24_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300620.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVP"
     gene            1067..1984
                     /gene="pstC"
                     /locus_tag="KPK24_002969"
     CDS             1067..1984
                     /gene="pstC"
                     /locus_tag="KPK24_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG2300621.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQGIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            1986..2810
                     /gene="pstA"
                     /locus_tag="KPK24_002970"
     CDS             1986..2810
                     /gene="pstA"
                     /locus_tag="KPK24_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG2300622.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            2829..3593
                     /gene="pstB"
                     /locus_tag="KPK24_002971"
     CDS             2829..3593
                     /gene="pstB"
                     /locus_tag="KPK24_002971"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG2300623.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            3621..4274
                     /gene="phoU"
                     /locus_tag="KPK24_002972"
     CDS             3621..4274
                     /gene="phoU"
                     /locus_tag="KPK24_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG2300624.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            4432..5301
                     /locus_tag="KPK24_002973"
     CDS             4432..5301
                     /locus_tag="KPK24_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG2300625.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            5388..6824
                     /locus_tag="KPK24_002974"
     CDS             5388..6824
                     /locus_tag="KPK24_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG2300626.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            6872..7699
                     /locus_tag="KPK24_002975"
     CDS             6872..7699
                     /locus_tag="KPK24_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2300627.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    7995..8257
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8333..10999
                     /locus_tag="KPK24_002976"
     CDS             8333..10999
                     /locus_tag="KPK24_002976"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG2300628.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     gene            11424..12134
                     /gene="rgaR"
                     /locus_tag="KPK24_002977"
     CDS             11424..12134
                     /gene="rgaR"
                     /locus_tag="KPK24_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG2300629.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNTITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT         4932 a         1299 c         2293 g         3968 t
ORIGIN      
        1 agattaagaa tttcttaata aatccagaag atgctttcga ttatataata ttttatattt
       61 ttatattatt aattatatgt gttgcaatcg ctgcacctac tttttctggt gagtatcaga
      121 cacaatcaga tagcatcctt cgttgtacga aacatggaca tattcgtcta gcgtttacta
      181 aaatattagc tctattttct atatttataa taatgtttgt tatatgtatt tctatccatt
      241 tggcaatttc tgatttagct tttggtactg aatgtttgaa aacctcattt caaatgttgt
      301 tttctgtcat tagtctagta aatataaatt tactacaagc acagattctt atagctattg
      361 gtggtctaat ttcaatactt gcaatggtta gtttaacatt atttttatct gcaaaatgta
      421 aggattctct tacttctatg ctgattgctt tttctatttg cttaattcct atgggaatag
      481 gtgttagttg gattggatac ctattaccac cagggggaat tggttttaag attagtttat
      541 tatatcaaat gacatcattt aatttcttac acttaggagg tatgagtttt tggacaccac
      601 acattatact gtttttctca gtgatagaaa tacctatttt tatatttttg gctattcgta
      661 cctattgcaa acatcaagta ccgtaacaat ttgatggaaa taatagtgat ataaactaga
      721 ggatacaata attatatatg tagtataaaa gttaaattta gttaacaaat cttaatagca
      781 gatataggta ataaattata cataaactgt attattatct atatctgcta ttttaatata
      841 gagaaacaac agtatattgt aaagtaaatg tcaaatatag ttttaaaagt aaaattaatg
      901 ttaaaaagta aatataattt tacacttgct taacttaatc ctaatataga tttaacttaa
      961 aaaaagtaga ataaaagtat aggaaatttg atgaagacga attataaaaa attaaatttt
     1021 gaatacagaa cgaaaatcga taagtatatt ttaatgggag ggtattatga tagtaaaatc
     1081 tagattagta ggtttagaaa aaaatgacaa taatggcaat aagacaaaat acgtaattga
     1141 aaaagtagca aaaaatattt ttctacttag tgctttagta gcagttgcaa gtttactttt
     1201 gataatagga ttcgtatttt ataaaggatt aagaccattt atatttgaag gatattcatt
     1261 tatagacttt ttaactggtg cagattgggt tccttctgcc aataaatttg gaatatcatc
     1321 tatgatagta gcatcaatat ttgcaacact tggagcactt gttataggag tacctgttgg
     1381 tatattaacc tctgtattta tagcagaagt agcacctaaa agattagcaa aaataatgtc
     1441 accagcagtt gaacttttag caggtatacc ttcagtatta tatggtgtat ttggtcttgc
     1501 agtgatagtt ccaactatac aaggtatatt taatcttcca aaaggtcaaa gtttgttagc
     1561 aatagtaata gtattagcag taatgatgtt accaacaata atatctgtat cagaaacagc
     1621 aataagagca gtaccaaaag catacaaaga aggttcatta gcacttggtg catcaaaaat
     1681 agaaacaata ttcaaagttg tattacctgc tgcaaaatct ggagtattag ccgcagtagt
     1741 gttaggagta ggaagagctt taggtgaaac aatggcagta atacttgttg caggcaactc
     1801 accagttatg ccaactgcaa taacagatag tgtaagacca cttacaacta atatagcact
     1861 tgaaatggga tacgcatttg gtacacatca agaaatgcta tttgcaactg gagtagtatt
     1921 gtttacattt atattaattt taaacttagt tttaaataaa ctttcaaata aggcggtaaa
     1981 ttaagatgag aaagttcaaa gagaatctat tgaagtcatt agtatatcta tcagcactat
     2041 ttacaatagt atcattagta gttatagtgg gatttatatt tataaaggga attggcaaca
     2101 tgaattttaa tttcttattt agcaattatt cagcatcagg tgatggtggt atacttccaa
     2161 tgataattac aacattatat acagtagtag tttctatagt tgtagcaact cctataggaa
     2221 tattggctgc tatatattta caagaatatg ctaaaaaagg aaaagcagtt acagctataa
     2281 gatttgcaac tgaaagttta tcagggatac catctatagt atatggatta tttggaggaa
     2341 ttttctttgt agttacatta aaaatgggat attccatagt tgcaggttca cttacagttg
     2401 caataataat attaccagta ataattcgta caacagaaga agctttaaaa acagttcctc
     2461 aagcttatag agaatcatca ttggcattag gagctacaaa atttcaaact ttatataagg
     2521 tagtacttcc aagtgcaata ccaggaattt tatctggagt aatcttatca gttggtcgta
     2581 taattggaga atcagctgca atattactta cagcaggtac tgtagccaag atgccaggtg
     2641 gaatatttga tagtgcaaga acattaactg tacactcata cttactgact aaagagagtg
     2701 gagatatagc aacagcagcc agtataggaa ttgttttaat agtaattgta ttagcactaa
     2761 acatgctagc aagatttgtt gctaagaaat taaataaagc taattattaa atttaagagg
     2821 tgtgaataat ggaattaatc gataagataa aaatgagtgt aaaagatttg gatttatttt
     2881 atggtgataa gcaagcatta aagaaaataa atatggatat aaaagagaat aaggttactg
     2941 ctttaatagg tccatctgga tgcggaaaat caacttttat aagaacgtta aatagaatga
     3001 atgacttaat cgaagatgta acaattaaag gaaacatttc tgttgatgga gaggacatat
     3061 atacctcaga tgatgttata aatcttcgta ctaaagtagg aatggtattt caaaagccaa
     3121 atccatttcc tatgagtata tatgataatg tggcatatgg tcctcgtaca catggactaa
     3181 gagataagaa gcaattagat aaaattgtag aagaaagctt aaaaggtgca gctatttggg
     3241 atgaggttaa agacaggctt aaatcatctg cactaggact ttctggtggt caacaacagc
     3301 gtatatgtat agcaagagca attgctatga gaccagaagt tattcttatg gatgaaccta
     3361 catctgcttt agacccaatt tcaacattaa aagtagagga acttatagag gatttaaaaa
     3421 aagactacac aatagtcata gttacacata atatgcaaca agctgctcgt atatcagatg
     3481 aaacagcttt tttcttaaat ggagaggtta tagaatttag cgatacaaaa actatgttta
     3541 ccactcctgt agataaaaga acagaagact atataacagg tagatttggt taaaatatat
     3601 aactataaag gagttagaca atggtcaata atagtttaga tataagtatt gatactttaa
     3661 aagaacaaac agtgcttatg atggaaaaat gtgaagaagc aatagaaaag tctgtgactt
     3721 gtatgataga gaaggattta gagggtgcaa gaaaagtagt agaagatgat gaagaaataa
     3781 ataaattaag agaagaaata agagataaga gtatagagct aatagtatta aagcaaccaa
     3841 tggcaaggga cttaagattt atttattcat taggtaatat atctatagag ctggaaagaa
     3901 taggagatta tgcagcaaat atagctagag aaatgttgaa gataggtgga gaggcttata
     3961 taaaagagct aatagatatt cctaagatgg caaaagaatg taagtctatg atgtctcaga
     4021 ctagagaagc tttaatctta tttgatgcaa gtatagctta tgatgttgga actaaagatg
     4081 atattgtaga tggattgtat gaacaagtac aaattgattg tttaaaaata atgcatgaaa
     4141 actcaaatac catagaccaa ggggtaaaac ttctacttat aggaagatac cttgaaagaa
     4201 ttggagacca tataacaaat gtatgtgaga agataatttt tgcaattgag ggtaaaatgg
     4261 ttgagatagg ataaaaaact gctttaaagc agttttttta ttgtctaaat taagaaagaa
     4321 ttgataaaaa aatatgatta atgggtaaat ttaaaaaatt gtgatatact aggaacatat
     4381 taaatatgag gatgcgtatt ttatataagg tgctttttag gaggtggaac tttgaaactt
     4441 caaaagatgg gtatgaggac tataaaaaca ggaattagtg tcactttatg tatgattctt
     4501 ggtgactatt tagtagagaa tatgttttat tctctggtag catgtataat ttctgttcaa
     4561 gatacagtaa aaggttcttt aaagacggga atcaatagag tggaaggtac tattttaggt
     4621 ggtataatag gattcatatt tgcaatcatt aaagctggag acccattttt atgtggtcta
     4681 ggagtaatgt gctcaatata tttatgcaat ctgtttaaaa taaagtcagt agtagttgcc
     4741 tgtgtaacct ttctggctat acatctaggt gtaggaaata gtgaccctgt gtattattct
     4801 ttacataggg tgttagatac atctgtgggt gttttgtttg gagtacttgt taattattat
     4861 gttgctagac caaattatct aaatagtgtt atagatgagt ttaaaaagat agaagaactt
     4921 tcaatgttgc ttattgaaga taaaattctt agaaaagaaa atttaaatgt agagaaattt
     4981 gaagaagaag tagaaaaatt agaagaaata tattcaaaac ttattgatga gttggattat
     5041 agtttgagtg aaactaatat agaaaaaatt gagaatgcct taaggatatg tagagaaata
     5101 cattttcata tgcagtctat agagttactt gaaaataaac tttatttgaa agagcataca
     5161 tataaatacc ttaagaaaac atataatgtg gataagctag actggaatat agatgaagaa
     5221 aaaagccctg tattcaacta tcatctaatg aaaataatga ttgaaataga gttgttgaga
     5281 aagctaaata aatcatatta ataatagtag ttaaataata aataaaaaat agtatctttg
     5341 taaatattaa acatatataa gtttaattta tggaggtact attttttatg aatactagaa
     5401 atgacaattc taaaatacta gaaatatata aaatgaagct ttttggcgaa cttgtaaaag
     5461 cagtgtggaa tgatgatata gaggatattg aaaacttacc taataaaatt ttaaatgaag
     5521 atttaaagga taatttaaat aattatttta gtggaaaatt tgataaagat actatggtaa
     5581 atttaattag ggtaactatg ggattggatg ttacagaaga tacagataaa aatttaaaag
     5641 agtcactgca tgaagctgta aatttagatg aagtcaaaag accaataata tctgtaatat
     5701 cagatgcttg caaatattgt gatgaaaata ataaggaaga tgagtcatgt aagataagaa
     5761 gtaagcatat taattgtaat gaggaagatg tatgtagtgc ttgtggtgat tgtgtatcaa
     5821 gatgtaagtt gggtgcaatt tcagataaaa tagaatttat accaatgata aaattgttaa
     5881 aagatataaa gagccctgta tatgcaatag ttgcaccagc ttttgtagga cagtttggga
     5941 aagatgtaag ccctggtaaa ttaagaactg cattaaaaaa gataggattt gaagatatga
     6001 tagaagttgc tttggcagca gatatgctta ctgcaaaaga agcatatgat tattatgagc
     6061 atatgaaaaa ggacgaagaa ggatgcttta taactagttg ttgctgtcct atttgggtta
     6121 gtttgataca aaatagtttt cctcagatat tggaaaatgt atcaccatcc gtttctccta
     6181 tgattgcttg tgggagagct gtaaaaattt taaatccaga tgctaaagtt gtattcatag
     6241 gtccttgtat ggcaaaaaag aaagaagcaa tgttagaaga tataaaagat gcagttgatt
     6301 ttgtgcttac atttaaagag ttgcaagaaa tatttgatgc attagagtta aatccagcaa
     6361 taatggatga gagtaataga gtagaggcgt ccctttcagg tagagtttat gctagaacag
     6421 gaggtgtaag taaagcagtt aagttatcag ttagaagaat tgacaaaaat ataaaatttg
     6481 aagaaaaggc atttgaagga acaaaagagt gcatggacgg tctaaagaaa gttctaaata
     6541 aagaaataga agctacattt atagaaggaa tggggtgtgt aggaggctgt gtaggaggtc
     6601 cgaagcgtat tttaagtgtg gaagaaggta ctaagaatgt tgatgaatat agtgaaagta
     6661 cgaatatgga aactccattt gataatttaa atgtattaca atttttaact tctttgggaa
     6721 tcaaaagagt agaatcttta ggagaaaaag aagaagaaaa ggttaaaaaa atatttagta
     6781 gagatataaa aagtaataag aaagagaata aatataaaaa ataacttttt aagtcttaaa
     6841 cttatgcttt tataaaaggg agatatgggg gatgaaaatt aaagataata aggctgtttt
     6901 tcatgtattt ataaggatat ttcttatagc tgttttaatt ttaactggaa cattattgga
     6961 ggaaaaaatt aataatgttc cagttgaaaa tataagttca aaaggtgaaa atacactgga
     7021 actgctttct caaagtaata ctttaggaga aatcatatac aaagcatctc aagaagaaac
     7081 aaaaggtcca ggggctactt ctgaaaaagt agcatacata acaatagatg atggtccatc
     7141 aaaatataca aatgcactac ttgatatttt aaaagaaaat gatgtcaaag cgacattttt
     7201 catgcttaat cagaatatga aaaaccacaa ggaagaagta aggagagtct tggaagaaga
     7261 aaactcaata ggttttcata gtgtaagtca tgatatacat aagttatata aaagcccaga
     7321 agtaacagtt ggggaattta atacatgcaa taatactcta caagaaataa ctggtcagag
     7381 ttcaaaatta ataagattac catacggaag taaaccatat atgccagaag gttcatataa
     7441 taaacttatg gcaaatgatt atttaatttg ggactggaat ttggatacac aggattggaa
     7501 gtcttctaca agtcagatag taagtaatat attatattat ggaagaaaaa gagatgaatt
     7561 agtagtatta attcatgaga aagagcaaag tttaaatgct ttaaataata ttataagaat
     7621 cttaaaagag agaggatata caatattgcc aattacagaa aatataacac ctaaaaattt
     7681 ctggcaagaa aacttataat aaatttaaat atagtaaaag ataaagtaaa ttatttataa
     7741 aataaaacat ataatataaa aaatacgaat aagaggtata ctgtaaggat aatataaaat
     7801 taatgagaaa gcattaaaat taaaataaaa taatcttgca attattgaca ttataagaca
     7861 gaataactgt aaaaacatat tttaaaatat aacaactata taaaagagaa tagacataat
     7921 taagaaaatt cagaaaaaat aaaataaaaa taattgacaa agagaataat atttgtatat
     7981 aataatatgt aatattaaag actgagaaga agaggagtaa gtaaatacta ctaaaagaga
     8041 ggaaaattca ctggctgtaa gattttctaa gtataagatt tactgaatgt agctttggag
     8101 tttctaaact gaactataag taagtttaga cggttagaac cgttaaattc tttgagagtc
     8161 aaagttgata aaataacatt aaccaataat ttaattatat gttaatgtat tttaagcttt
     8221 ggaattaagg tggtaacgcg agcttttcgt cctttttaaa gaggatgaag agctcttttt
     8281 tatttctaaa gatatttaaa aaatttattt ataaaataca ggaggcagga caatggaaaa
     8341 tacgaattta tcaaaaactt ataacccaaa agattttgag gctaggctat ataaaaaatg
     8401 gatggatgaa gggtatttta aatctaaacc aaatccagat aaaaaaccat ttacaataat
     8461 gatgccacca ccaaatataa cagggcaatt gcatatggga cacgctcttg accatacact
     8521 tcaagatata ttaatcagat ggaaacgtat ggatggatat gaagcttttt ggcttccagg
     8581 aactgaccat gcttctatag caacagaagt taaagttgtt gaaagaataa aaaaacaaga
     8641 aggcaagaca aagtatgaaa taggtagaga agaattttta aagagagcct gggaatggaa
     8701 agatgaattt ggaggaaaaa tatctaatca gcttaaacaa ctaggtgatt cttgtgattg
     8761 ggataaagaa agatttacta tggatgaagg gtgtaatgaa gcagttatag aattttttgt
     8821 aagcctatat gaaaaaggtc acatatacag aggaaataga ataataaact ggtgcccaga
     8881 ttgcaaaact actttatcag atgcagaagt tgaacatgaa gaacacgacg gaaactttta
     8941 tcatataaag tacccactaa aagatagtga agacttttta gaaatagcta caactagacc
     9001 tgagacaatg ataggagata ctggtatagc agtaaatcca gaagatgata gatataaaca
     9061 tttaatagga aaaacagcaa tacttccatt agtaggaaga gaacttccaa tagttgcaga
     9121 tagttatgtt gatttagagt ttggaactgg tgcagttaag atgactccag cacatgaccc
     9181 taatgatttt gaagtaggat taagacataa tcttgagcag ttaaatacta tgaatgaaga
     9241 tggaactatg aatgaagttt gtggaaaata tgaaggtatg gatagatttg agtgtagaaa
     9301 agctatcgta gcagacttaa aagaacaagg ttacctaatt aagataaaag agcataatca
     9361 taatgtagga acttgttata gatgccatac agtagttgag cctagattat cagagcaatg
     9421 gtttgttaag atggaagaac ttgcaaaacc agctatagat attcttaaaa aaggagaatt
     9481 agaatttgtt ccagataaat ttgataaaac ttatttacaa tggctagaaa atataagaga
     9541 ttggtgtata tcaagacaat tatggtgggg tcatcaaata ccagcttatt attgtcaaga
     9601 gtgtggagaa atagtagttg ctagaaaaat gccagataag tgtcctaaat gtggaagcac
     9661 tcattttaaa caagatgaag atgccttaga tacttggttc agttctgcat tatggccttt
     9721 ctctactcta ggatggccaa ataagactga agctttagat tattattatc caacaagtgt
     9781 acttgttaca ggatacgata taatattctt ctgggtagtt agaatggcat ttgcaggtat
     9841 gttctgtatg aatgaaaagc catttgacca tgttttggtt catggattag taagagattc
     9901 tcaaggtaga aaaatgagta aatctttagg aaatggtatc gacccattgg agataatcga
     9961 gcaatatgga gctgatgccc taagatttac attaacaact ggaaattccc caggaaatga
    10021 tatgagattc tatatggaaa gagtagaatt tgctagaaac tttgcaaata agttgtggaa
    10081 tgcatcaaga tttgtattta tgaatattga tgaagatata attaaaaaca tgactagaga
    10141 aagtgttaaa gaggacttaa ctctagctga taaatggata atatcaagag caaataatat
    10201 tgtaaaagaa gcaactaata acatggataa gtttgattta ggaatagcac ttcaaaagat
    10261 atacgatttt acttggtcag aatactgtga ctggtatatt gaaatggtta aacctagatt
    10321 atatggggaa gatgcaaatg ctaaatctgc agcattatat acattaactt atgttcttga
    10381 aaagatatta aaattattac atccatatat gccatttata acagaggaaa tatatactca
    10441 tcttcctact gttgaaggat gtataatagt atctgaatgg ccaaaatata atgaagaaga
    10501 taacatggct gaagaagaag atatgatgaa cttattgatg gaaggtataa gaagtataag
    10561 aaatgtgaga gcagaaatga atgtaccacc ttctaagaaa gctaaattga taataatccc
    10621 aagtgaagaa aaaatagaag ctattgagct tggtaaagac tattttataa ctttagcatc
    10681 tgcttcaaat gtagagatag caaaagataa atctaatgtt ccagaagatg cagttggagt
    10741 agttattgat ggtgttgaaa tatttatacc tcttaatgaa ttagttgatt ttgaaaaaga
    10801 aattgagaga ttatctaaag aaaagaaaaa gttagaaggc gaaataaaga gagttaatgg
    10861 aaagcttgca aatcaaggtt tcttagcaaa agctcctgaa agtttaattg aagaagaaaa
    10921 agcaaaaaaa gaaaaatttg aagagatgat taaatctgtt gaggaaagat taacaaattt
    10981 agaatctaaa ataaaataaa tataagaatt agtaaaaaca tacaaaaagt atatattcta
    11041 tactttttgt atgtttttct ttatatatat acaaatatga tgttataatt ctttacagat
    11101 gtgttatatt tttattttat gttgtgaata tatgaatttt atgtcttaaa atttataagc
    11161 aataaaaatt tgatatatta tgatgtttaa tatagggtaa taaatacata ttatatttat
    11221 tttactatat ttttttacat aatatatcta gaaaagtata ggaatttatc gtaatttaag
    11281 tttaaatatt aaagagaatt tctataagta gtttaatcga caatttaaat ttataaagaa
    11341 atttaaattt gaaagctagt gagtacagtt tggaaattga aataaagtaa actaaatgat
    11401 attttagatt tggaggtgat attatgttca gggtagttat atgtgatgac gaaaaaatac
    11461 aaagaagcat actaaaagag tttacagaaa aacttttgtc tgaaagatgt ctaaattatc
    11521 aaatactgga attttcctgt ggagaagatt taatatccaa atacccagaa aaaattgata
    11581 ttatattttt agatatccaa atgaaagata tcaatggaat tgaggtggct aaaaaaataa
    11641 gaaagtttga tgaaaaagta gaaattatat ttacaacggc tttttcagag tatgcccccc
    11701 aaggttatga agttcgtgct tatagatatt tagtaaagcc tatagagtat agtaactttg
    11761 caatgggtgt aaatctttgt attgataacc ttcaaagaaa gaaggagcgt tatatagttt
    11821 taaatagtaa aaaaggattt aatagaatac tgatagattc tattttatat gttgaaactg
    11881 taaagagaga tttagttgta catacaatag gtagagatta taaagctaat atgagcatga
    11941 aacaagcaga aaaattactg tgtgaaaatg gcttttttag atgtcataca agttttattg
    12001 ttaatttaag aagaatagaa gatcttaggg ataatacgat tactattaat gaacaattta
    12061 tacctataag taagtataga cttaaagatt taaaagtagc attaacaagc ttgttatgtg
    12121 atataatgta ttaattttaa aaaggtaatt gaaaatatcg taaaaaagat ttaatgtcat
    12181 cacagggcta ttgattgaaa ctgtcttaat atataaaatg ttatttatat taagacagtt
    12241 ttattatgca aaatagcagt ttatagactt ttaatatggt ttaatatgta tatgtagagt
    12301 ttccattata atagcatgat tattaattag tagcttgatt atcaatatac tattattgat
    12361 ttttatatgt attatatgta tttttagaat atgattacct ttttaaaata atgactattt
    12421 ataaaactct atagatattt gtttcattta gctatgcata ctttaattaa ttatattaat
    12481 atttaatgga gt
//
LOCUS       DAEMGG010000115        12444 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEMGG010000115 DAEMGG010000000
VERSION     DAEMGG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.116"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            98..286
                     /locus_tag="KPK24_002978"
     CDS             98..286
                     /locus_tag="KPK24_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300630.1"
                     /translation="MNYHTKEELVEALRVISSSIINCEKGQKKFSEETSHHTCFKNII
                     EAIYISKSLIMDEISKRD"
     gene            546..1745
                     /locus_tag="KPK24_002979"
     CDS             546..1745
                     /locus_tag="KPK24_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG2300631.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1797..2300
                     /locus_tag="KPK24_002980"
     CDS             1797..2300
                     /locus_tag="KPK24_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2300632.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            2407..2724
                     /locus_tag="KPK24_002981"
     CDS             2407..2724
                     /locus_tag="KPK24_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300633.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            2882..3841
                     /locus_tag="KPK24_002982"
     CDS             2882..3841
                     /locus_tag="KPK24_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2300634.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            3956..4741
                     /locus_tag="KPK24_002983"
     CDS             3956..4741
                     /locus_tag="KPK24_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300635.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            5033..6352
                     /locus_tag="KPK24_002984"
     CDS             5033..6352
                     /locus_tag="KPK24_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2300636.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(6427..6855)
                     /locus_tag="KPK24_002985"
     CDS             complement(6427..6855)
                     /locus_tag="KPK24_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300637.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNAVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(6946..8322)
                     /gene="hydG"
                     /locus_tag="KPK24_002986"
     CDS             complement(6946..8322)
                     /gene="hydG"
                     /locus_tag="KPK24_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG2300638.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(8323..9408)
                     /gene="hydE"
                     /locus_tag="KPK24_002987"
     CDS             complement(8323..9408)
                     /gene="hydE"
                     /locus_tag="KPK24_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG2300639.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(9444..10652)
                     /gene="hydF"
                     /locus_tag="KPK24_002988"
     CDS             complement(9444..10652)
                     /gene="hydF"
                     /locus_tag="KPK24_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG2300640.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDSGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(10667..10918)
                     /locus_tag="KPK24_002989"
     CDS             complement(10667..10918)
                     /locus_tag="KPK24_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG2300641.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(11299..12339)
                     /locus_tag="KPK24_002990"
     CDS             complement(11299..12339)
                     /locus_tag="KPK24_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG2300642.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         4596 a         1529 c         1884 g         4435 t
ORIGIN      
        1 aaaagatgtg tataaataat aatgatgtca gtttataatg atattaaatt gaggtatact
       61 gtttctctaa attaaaaaat aaaggagtaa atgtgttatg aattatcata caaaagaaga
      121 attggtggaa gcactgagag taatatcttc aagtattatt aattgtgaaa aaggacagaa
      181 gaaattttct gaggagacat ctcatcatac atgttttaag aatattatag aagcaatata
      241 tatttcaaaa tcattaataa tggatgaaat tagtaaaaga gattgaagct tattttgcaa
      301 tttaacatgt tttaatatac attttattat ataagaaaat aagtaaatat acatgttata
      361 aaatattgga ataatataca attataaatt tgttaacatt aatatctata tgttaaaata
      421 tagtagaaga tgttagtaaa taataggatg agttctattt taatacaatt aaattaagct
      481 tagtttgaaa tttgtatcta aaaagtaaag taatctagaa ttaagaaaat ggagatagaa
      541 ttataatgga aaaattatat tatacaaatc agtatataag agattttaca gcagaaataa
      601 ttgaaataaa agaagtagaa aataagtatc atgttttatt agataagaca gctttttttc
      661 ctggtggagg aggtcagttt ggagacctgg gaaaaataga gaatgatgat gtaataaatg
      721 tgtatgaaga tggaaaaaaa atataccatg tattagataa aaaacctata aagatacata
      781 aggtaaagtg tagtattgat tgggatagaa gagaagatgg aatgcatcaa cattttggac
      841 aacatgtatt gtctggatgt ttttttaaaa cttttaatgc aaatacgaca ggttttcatt
      901 tgggaaatga gtatagtaca gtagatatag aaggtaattt agaagaggat aaaattagag
      961 agatagagtt gtttgccaat gaaattattc gtcaaaatat acaagtagag acattacttc
     1021 caacgaaaaa agaactaaaa aagatttggt taagacgtga tttgccaaat actgatgaag
     1081 agataagagt aataaaaata ggtgatttag atacgaatgc atgttgtgga gtgcatccaa
     1141 aatcaactgg tgaattgaga atgataaaaa taaagaaatg ggaaaaacat aagtcaagta
     1201 caagaataga gtttctagca ggtaaaaggg cagtggatga agtattgaag agggatatat
     1261 atttgactaa aatatgtaga tatttaagct gtagtgagga agatgctata aatggtattg
     1321 taaatctaaa tagtaaagtg gaagaggctt taattaaaaa aagaaggctt gaagaagtag
     1381 tatctacata tcaggtcaaa gaaatgattg aaaaagcaaa taaggttggc aatatttcgg
     1441 tcattaaaaa aatttatgat gatgaagatt tgaaatatgt aaataagata gcaaataaaa
     1501 ttgcagaata tgaaaatagt attgcattat tagctgttaa atttaatgat aaaataaatt
     1561 tagtttttgc atgttctaaa aatttaaaag atattaatat gggagtttta ttaaaggatg
     1621 caataaatct tatagatggc aagggtggtg gaagtaaagt attagctcaa ggaggaggta
     1681 agaataatgg aaatttagag gctacacttg attatgcatt tatgaagata gaaaaaactc
     1741 tataaatata ttatataaaa aaccttagta gataaaatag atatggagga ataagtatgg
     1801 agcaacaaaa ggactacata gagttatatg aaaatgaaga actaatagag ataagagaag
     1861 cacgtatgga tgatttggat acaatagcaa aatttaatta taatttggct aaagaaactg
     1921 aaggaaaaga acttgatatg gatgttttaa ctaaaggtgt aaaagcattg ttattagatg
     1981 agagaaaagg aaaatatcat gtatatacag tatttgataa agttgttgca cagattatgt
     2041 atacatatga gtggagtgat tggagaaatg gaaatttcct ttggattcag agtgtatatg
     2101 tagataagga atatagaaga aaaggtatct ttaattattt atttaattat ataaaaaata
     2161 tatgtgataa agatgaaaat atagttggaa tgagattata tgtagaaaaa gaaaacataa
     2221 atgcaaaagc aacatatgag tctttaaata tgtatgagtg tgattataat atgtatgaat
     2281 acgaagtagt acattcgtaa tataaatttg taatactgtt atactgatgt tataaagtat
     2341 tgattatatt gacgattata tgaaataaac tggatattat aattagaatt tttagggggt
     2401 ataaaaatgg gcgttttaaa aggaatgttt tatgtatttt tatttttaga tttagtgtca
     2461 atattctgtt tctttttcaa taaaggaaaa atagcatcta ataaaattgt gtttaatgca
     2521 ataggagtac ttacatttgt actttgtttt atgcttttta gttattatcc caataataat
     2581 cttattggaa aatttattgc ttctctattt tttatatttg gagttgcagg agttatgttg
     2641 aaagaaaaaa attttttata tgctagactt cttttaactg tagttatagt tttttctaca
     2701 ttaagattgt tcgttattca ataaaatgta tttgtatgaa aatttattaa agtctacaca
     2761 tttaagtgta ggcttttttg attttttgtt tgaaaattaa ggaaatttta aggaaatttt
     2821 aaaatcgtct taaatattta ttgatatact taaaatatta atagcgaaag gagacttatc
     2881 agtgatagaa agtaaaaagt cagaaaaatt gttactgtca attatataca taatattttt
     2941 ggtatatatt ttaatgatta taagaataat tttatttaga gatgtaccaa tttacgctat
     3001 tttcaaggga acgtttagga atgtaaattt aattccattt tatacaatat atcaatttat
     3061 agtagatagt aatcttgact ttatgaaagc tactgtaaat attatagaaa atataggtat
     3121 atttattcct atgggaattt ttttacctat agtatgtaaa aatctaaata aaaaaactat
     3181 aattatagta attatacttg ttagcctagc atttgaatta acacaatata tttttgctct
     3241 tggaagtagt gatatagatg atgttatttt aaattcatta ggtggcataa ttggaattaa
     3301 tatttacata aacatgaata aattatttcc caatgatatt aacagattta aggctataat
     3361 aggtactagt ttagttttag gagttatagg attaggagtt atttctaaaa attatcataa
     3421 cttactaact tttaaattta gacctgatag aaaaatatca aaaatattaa ttgaagaaaa
     3481 tagggaaata attaaagata taaataaaga tacagtagat atagttggga catttgagag
     3541 ctttaaaaaa ggaataataa ctattaaagc aggttctaat aacaaagtaa agagaccaga
     3601 gaatttaatt gattctgatg gaaatataag aatttattta aatgaaaata caaaattagt
     3661 atcttatata attaccaaag aatcaaaaat ggatatagtt aaatatgagg aatttgatgt
     3721 gaagaattta gatttattaa ggagatatga tactattaat gtttggattg ataaagaaaa
     3781 tcagccaaag gataaacatg gtattatggc tagtaaatta ttaattggat tgtatgaata
     3841 aaatattaaa caagaataaa agaataacaa atataaaaaa cgaaaaaact atttctataa
     3901 ttatttttac gttgtgcata aaataagaac attagtgaag gagtagagat aaattatgga
     3961 gataataaaa tatatcttgt acttttttat atattcattt ttaggatgga cagtagaaag
     4021 tataggatgt tctatagcaa gcaaaagaat aatcaacaga ggttttttaa atggtccaat
     4081 atgccctgtt tatggttttg gagcggtaat tgtgatatca ctattgggaa gatttaataa
     4141 tgtagtaata gtatttctat taggaatgat tttaactacg atactagaat attttacagg
     4201 atttatttta gaaaccttat ttcatgctaa atggtgggat tattctgaca gaaaattcaa
     4261 tataaagggt agagtatgtt taaaaaatgc aatatacttt ggtgtaatgt ctgttttaat
     4321 aataagattt atacatccat ttataaaata ttttgtaagt ataattccat atagaatatt
     4381 aatttcaatg gctataatta caacactttg gactatactt gatttgattg ttactattat
     4441 aacacttaaa aaattagata taaagttaaa cttattagac gatataatta cagaccttaa
     4501 tgatataaat gtgaaattgg ataaatttga tagaggagaa atacaagctt tatttaaaac
     4561 aataaataac gacgaattag aagttagaga aaaaataaac aaaataaata gtaagttgga
     4621 ccgtatagag tctaatatta ttttacaaaa aagagttata aaagcttttc cacatataaa
     4681 acataaaaaa caacaagagc aactagaaca ttttaaaaaa attattaatg agaaaaggta
     4741 attatacaat ttaatgaaga cgaatagaat aatggtattt ataagatgat tttcagagga
     4801 ctctataaaa atagaataga gtcctttatt ttttgaaaaa taatgtttat ttgataaaaa
     4861 taaaatatat tgtttctgaa taacaaatat tttgaaaatt ataaaaacat atattgacaa
     4921 tttgtgtcaa aaatactaac attaagtata taaagaattt tatatgagaa tatctttata
     4981 aatcgcaata attttaaaac ataaatttat ttaaagaggg gatgatttgc caatgagcga
     5041 gataactagt caaatgataa gtacagaaga aaaaaaatat gttgctaaaa ctcaaaagat
     5101 accttattat ccagtagcat ttaaatcagg tgatggtgca atgctatatg attatgaagg
     5161 taatgaatat gtagattttt tagcaagtgc aggctctgca aatgtaggtc atggtaataa
     5221 ggaaatctca caagcagtta aagaacaaat ggatgatata acacaatata cacttgcata
     5281 tttccatagt gatcctcctg taaaattagc tgagaagctt gtggaaatag cccctggaga
     5341 taatgataaa aaagtactat acagtgcaac tggttctgct tgtatagatg ctgctataaa
     5401 attagcaaga gggtatactg gaagaacaaa gataatatct atgtgtgaat cttatcatgg
     5461 aagtacttat ggagctatat ctatatctgc tttaagtaca aatatgagaa gaaaaatggg
     5521 tccattgtta ccagaagttt atcacttcca ttatcctgat aaaaatagaa cagctaaaga
     5581 atgtttagat gagatagaat atgcttttgc acactatcta ccagcagaag aggtagctgc
     5641 aatatttata gagccaattg caggagatgc aggaataata gtgcctccag tagagtgggt
     5701 acaaggttta agtaaaatat gtaaagaaaa tggaatatta cttgtaagtg atgaaataca
     5761 acaaggtatg ggaagaacag gtaagtggtt tggtatagaa aattttggtg ttgaagcaga
     5821 ccttatagtt cttggaaaat ctgttggagg tggattacct ctaggagcag ttgttggaag
     5881 aacagaaata atgcaaagtc ttgatgcacc agcacattta tttactttag ctggaaacac
     5941 aacagtatgt gtggctgctt taaaatctat agagataatt gaaaaagaaa acttacttca
     6001 aaagagtata gaaatgggag attatattaa agcaggattt gaaaaactaa aagaaaaata
     6061 cgatataatt ggagaaataa gaggcatagg attatctata ggagttgata tagttaaagg
     6121 aaaaggttct aatgaaaaac atccagatgc tacagctaag atatgttata gatgtataca
     6181 aactggatta ataatgatat tcttaggaca atctacttta agagtgcaac cacctctagt
     6241 aataactaag gagcaagtag ataaagcaat gaacatcata gattctgcaa tagatgatta
     6301 tttaaatggt agaattggtg atgaagtata tgaagtaact caaggttggt aaaataaata
     6361 ttaaatgaaa ataggagtta tctcaatttt agaaaattat ttctgaaaag agataactct
     6421 tttaagttac ccacaaataa gaaaattgtt tgattcatta aagactatat ttcggatgtc
     6481 gtttttgtga gtatcagaga cagcattacg agaataatta cctttattcg tactatctgt
     6541 taatttagtt ataattaaga tagctaaaag aataagaatt actgtgtata aagcaataac
     6601 atatggtttt cttttatatt tgtatttgtt attgaaaaaa gaatggccat aattattttt
     6661 atttacttta gatgtatttg tattcttttt cttgttacct ttctttttat catccttttt
     6721 tctattttta gggtaaggaa tgactttgtg tgtattatca agtttgatat ttttattttt
     6781 ttgaatattg ttttccaggc tagccttttt ttctccaaat tgaatagttt tattggtatt
     6841 gttgttatct ttcaatgtta atctccctcc cttaaacttt ccctttagat aatattacta
     6901 ctaacctatg gaatatacaa taaataatat ataagtaata aaattttata ggtataaatc
     6961 tctttctcca cgctctaagg cagtcatatt gttagatact atgtttctta tatcttctct
     7021 ttctatctta cctatttctt ctgcgataag ttttttaccc ataattttaa gttcttcatc
     7081 tccataatcg attaaaaatt catttaatgt agtaagagca tttggtgtac atacattatg
     7141 gatttgtcga cttttagcta agctcatgaa tctatcgcca gttctacctt tacgataaca
     7201 agctgtacaa taactaggta tatatccatc agtaatcagt tctttaagca cctcaacagg
     7261 gcttctatgg tctccaactt cgaattgttc tgtattgtca ccatcttcat atgctttata
     7321 tcctccaaca cctgttagag aacctgcact aatttgtgat acaccatatt ttagtaactc
     7381 atttctcatt tcagcagttt ctctagtgga cattatgatt ccagtaaaag gaacagccaa
     7441 tctagttata gctactattt ttttgaacat atcgtcagaa actaagtgag gaaaatcttc
     7501 taagctcata ccttcagctt ttttcaatct agggaaagat atagtatgga aacctacacc
     7561 aaatttttcc tctaagtgtt cgttgtgcat cataagtcct agaacttcaa atttagggtc
     7621 agataaacca aataaaactc ctgcaccaac atcatcaaca ccagcttcca tagctctatc
     7681 aaaagctgtt aaatggtaat aatagtcatt ttttatagat tgaccatgca tttttataaa
     7741 tgttggtttg tgataagttt cttggaataa tatataagtt cctattcctt tttcttttag
     7801 taatttatac tcatctacac ttgtagctgc tatgttaacg tttacccttc ttatatttcc
     7861 gttttcatta tatgttgaat aaattgcatc taagcaatct aagatgtaat ttatatcaca
     7921 atttttaggg tcttcaccag cttctagagc aagtctttta tgacccattt tttctaatat
     7981 tttaacctct tcctttatct cttccatggt aagttttttt cttttaaatt tgttgcattt
     8041 actaaaaccg caataaacac attcatttac acagtagttt gatacataaa gaggggcaaa
     8101 taaaaccact ctattaccat atatttcatt ttttatttgc ccagctatac taaataatct
     8161 atctagttgt tttttatctt ctacttcaag aagtgtagct atatctttat aactaagttt
     8221 ttcccttcta tcagccttat caagtacctt ttcaatatca tcagaagttg aattttttgc
     8281 atcttctaat aaagaattaa taagttcatg gtttatgaac atttacaaac gcctccattc
     8341 aatattatca ccgcgagaat agtcaacttc aagtcctaat gacttgatgt tttcttctaa
     8401 actttggtga tattcacaag cttcttgacc agtaaaggct ttgttatcgt aaagagaata
     8461 ttgttttcta aattcttgag gagaaaggtt tggcataatt acattgcaac cagctaaaag
     8521 acctgcattt ctaccactag ggtttataga tgccaaggct gttgtagcgg gtaataaaac
     8581 ttttggaagt agaagtcttg taattgaaag cataagtaat gtcttctcta aatctccatg
     8641 tttataatcc ttaaaaattg tatcatggtg tggaataaaa ggtcctattc caaccatatg
     8701 aggatttaac tcttttaggt ataataaatc acgaacaagg tcttcgttgt tctgaaatgg
     8761 tgattcaacc ataaatccag cacctacctg ataaccaatt tctttaaggt tttttaggca
     8821 ttcttttcta gttctaagct caatgtttgg cggatgtaat tttttataat gactgtctgt
     8881 agcagtttca tgtcttaaaa gatatctatc tgcaccacat aaaaagtatt ttttatacga
     8941 atcataactc ttttctccta gtgatagagt tatggcacaa tcctcatatt tattttttat
     9001 acttgaaaca atttcacata ttttatcatc agtaaaatat gcatcttcac caccttgcaa
     9061 aacgaatgtt ctgtatccta ttttataacc tatatcacaa cattcaagta tttcatcaag
     9121 tgaaagcctg tatcttatga tatttttatt agaagattgt attccacaat agtagcaatt
     9181 atttttacaa tagtttgtaa gctctataag tcctctcaag tagactctat ttccataatg
     9241 tttatttctc atgtcgctag cttgtttata aagatagttt cttaaatttt gttcgaagtt
     9301 atattcctca ttgtcgatat tttctattaa atatagtagt tcatcaaaac ttaatgaatt
     9361 aatgctgtaa agtttatcta taaagtattt aatcttattt ttagtcatat tcaaatatat
     9421 aattattaag tatagttaaa tttttaataa ttgaaaagtt caagtgatct ttccaaaata
     9481 ccttttacat atgctattaa gataccatag tttacaatag gtacattgaa agattttgcc
     9541 ttttctattc tagataacat tccagctcta ttcatcatac aagctccaca gtgaactaca
     9601 agggcatatt tatttatatc ttcagtgaag gatactccag aactaaattc aaaagtaatg
     9661 tttttgccag ttttctttct aatcatatta ggaattttta ctgttccaat atcatcagtt
     9721 tgtctatgat gggtacatcc ttcggccatc aagattttat caccatcttt taaattttct
     9781 aaagcataag cgccattaat caattcttta aggtctccct tttgtctagc aaacaatata
     9841 gaaaaagatg taagaggaat atcttttggt gtatctttat ctacttgtgg aaatacttga
     9901 gagtcagtaa taacaagttt tggttttttg cctaaattag atagagtttc ttttaaacta
     9961 tcttcttttg taactataga aattgcacca ctatccaaaa tatctcttat aacttgctgt
    10021 tgaggaagta taagtctacc ttttggagct gctttatcta ttggaacaac taaaacaact
    10081 aaatcatttg gttcaattaa atctgatacc aatttgaact cagtgctatc cttaggcaaa
    10141 acttttatta tttcattttt taaattttca attccaattt tatctgttga tgagacagaa
    10201 acaactggac atttaacttt atttttagtt aagtttaaaa tttctgattg attcttaata
    10261 gtatcaattt tgttgagaat tagaatatga gggatattct tatcattaaa cttttcaatt
    10321 agtgacaaat cttcttgtga tataccaatc tggcaatcca ctactagtaa agctatgtct
    10381 gtcttttcta aaacatctag tgatttacct attctaagtt caccaagttc accaacatca
    10441 tcaagaccag cagtatctat aagaacacaa ggtcctatag gaaggatttc cattggtcta
    10501 aatactgggt ctgtagtagt tccagcaata tctgagacta ttgcagcact ttgatttgtt
    10561 atagcattta ttatgctaga tttacctgca tttcttttac caaagagacc tatatgtact
    10621 cttaccgatt gtggagtaga atttagactc atttgtactc accaccttaa acttcttttt
    10681 ttgatatact agtttttaca gatacatgag gtatgttgcc taatttacca gtcaaactat
    10741 taatagtatc catttcgcca acaactgcta tacatacgat tgagacacct tcttcttcgc
    10801 aaggaatgcc cattcttcct ttaataatac ctttaaaacc tgatacaacc tcattgaatt
    10861 taaattgaca ttcttttggc tcttccaaaa tagcacttat aactgcaacc tttttcataa
    10921 atatcctcct aactcagaaa atatgtctta aacaaatcca aaatcaaaaa acttttcttt
    10981 gtaaagaaaa gtttctacaa aagaaaagtt attaattaca caagtataat gtgctatatt
    11041 aaactatttc cttacagatt agataactct tttctggtag ggttagcttg taaaaattta
    11101 atttaacaac aagtaactga agaaaatttt tatacaattt atataaatta taataaaaat
    11161 taactaaaga aaattgaagt tacttatata atagtagtaa attgaaatta agtctataga
    11221 aaaaccaatt tttgtataaa aataaaaatt aaagttaatt atttgttaag aattgacttt
    11281 ggtaaaatat ataattaatt attctttaag catataattt ataagtaatt ttagtgtatt
    11341 ttcaatagct tctatatgtg ttctttcata atggtgacta ttgtttgttc caggaccaac
    11401 acatgctatt tgaacatctt ctccccaacg aatagcttct gaagcatctg aaccatagaa
    11461 agtaaataca tcgacagcgt agtctaaatt attgtcttct gcaacatcga caagcttgtt
    11521 tcttaaatta aaatcatata atgataaatt atcttttgca actatagtta caccatgttc
    11581 actagatgtt tgacttggtc cagtaggtgc tatatctatt gctacaaatt cttttgtttt
    11641 ttcaggtaaa catttagaaa caccatgtcc aatttcttca taattactta tataaaagtt
    11701 agtagtatgc ttaggtgtta aattgttttc tttaaaatat ctacatattt caagaaccat
    11761 tgctacagct gatttattat ctatatatct agattttata aaaccagatt ctgtagcctc
    11821 aaatctatgg tctaggcata caaaatcccc aacacttatt cctaaatcta atacatcttg
    11881 ctttgtaaat actttttcat caagtctaat aatcattgtt tcttcatttc tttcagtggc
    11941 agcttttgtt tgaccatata tatgagtaga agcatactta aatacaacac ttcctctgat
    12001 ttttttacct tttctagtta taacagtaca gttttctccc tctatagctg cccaacatcc
    12061 accaccaact ttatggtatt taagagtacc atcagatgat atttctttta ctatagctcc
    12121 aagtgtatcc atatgtgcag atattgttat ttgatttgaa tcatcttcac caggaagagt
    12181 agctataagt gcaccttttt ttgtaagaat agtgtcaaga cctaaatttt caaaatcttt
    12241 tttgatttct aaaactgcat tttcagtata tccagctggg cttggaatgt ccaaatattt
    12301 tttcattaaa tttatagtat tagttaaatt taaattcata ttgtttcctc ctaaataaat
    12361 atatttatac aaattataac ataaatacaa atgttctgaa aactaaaatt aaaagaatag
    12421 aatctaaaca tatttaaaaa aaaa
//
LOCUS       DAEMGG010000116        12405 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEMGG010000116 DAEMGG010000000
VERSION     DAEMGG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.117"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1026..2381
                     /locus_tag="KPK24_002991"
     CDS             1026..2381
                     /locus_tag="KPK24_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG2300643.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
     gene            complement(2607..3488)
                     /locus_tag="KPK24_002992"
     CDS             complement(2607..3488)
                     /locus_tag="KPK24_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG2300644.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTILSGEIMY"
     gene            complement(3475..4227)
                     /locus_tag="KPK24_002993"
     CDS             complement(3475..4227)
                     /locus_tag="KPK24_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG2300645.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            4494..4811
                     /gene="trxA"
                     /locus_tag="KPK24_002994"
     CDS             4494..4811
                     /gene="trxA"
                     /locus_tag="KPK24_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG2300646.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            complement(5018..6292)
                     /locus_tag="KPK24_002995"
     CDS             complement(5018..6292)
                     /locus_tag="KPK24_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG2300647.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGINPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            7178..8014
                     /locus_tag="KPK24_002996"
     CDS             7178..8014
                     /locus_tag="KPK24_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2300648.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            8367..9944
                     /locus_tag="KPK24_002997"
     CDS             8367..9944
                     /locus_tag="KPK24_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2300649.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGIANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            9937..11121
                     /locus_tag="KPK24_002998"
     CDS             9937..11121
                     /locus_tag="KPK24_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG2300650.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            11139..11738
                     /locus_tag="KPK24_002999"
     CDS             11139..11738
                     /locus_tag="KPK24_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2300651.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            11762..12232
                     /locus_tag="KPK24_003000"
     CDS             11762..12232
                     /locus_tag="KPK24_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2300652.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         4508 a         1412 c         2006 g         4479 t
ORIGIN      
        1 tttaatttaa attttttatt ctattaatta ttaatattat tttcaaatca atgcagttat
       61 aagaagtgta taatttgatt attatatact gcatacaaaa atatatttga gcaaataagt
      121 aaagtaataa aaaaatcaag atttcatatc taaaaacaat tctgaacatt aatttaattt
      181 atattttcat aggaaatatt ttatataata tacttgataa atataatttg aatttttcta
      241 attatttact gtcgattaat agattttttt cttgcatata agtgcatatt tatggtacga
      301 tttacatggt gaaataaaaa aggggaggtg aaaaataaat attcgtgaaa aagttttcag
      361 tttgaggtat tttatgaata atatattttg aggtatttta taaataatat attatacata
      421 aagagtataa cctcaataaa tagtatcaaa gcgaatatat gggtaattac tttaaaataa
      481 cagtttttat gtttagattc acagtgttta ttataaacta atgtgtaaga aataagccta
      541 atctgtgaat agatagtagt ttagttattt aagttgagaa ttggttgagt gaagtaatat
      601 ataaatttaa aaaataaata aaaacattat ttgaacaaaa tatatgttgc tttattcgag
      661 actcctttaa aaaagcacta aaatgtaatt acataagttg ctacaaaagg gggattttag
      721 tttgtaaaat actatcacgt aaaaaataag aattgcatat ggcgaaggtt ttttcatggt
      781 attagatgag aatatattaa tttataacta tatctgaaaa tagtgaattt atataattta
      841 aatttatgat taagaagtgt attaatttta ttccatgaga atatgtactc tacaaataaa
      901 tgcgcaatta tcttaataat aattacaaat tgttttacaa aataataata ttatatcttt
      961 cataaaagaa agaatttgga caaaacgtaa aaaataaaaa tacaattaag aaaggattta
     1021 aaattatgag ctcaacagta aaaaagaagg gtaaatttcc tatgggattt tatatctgct
     1081 caacaacttt ctcatttgaa agagcagcat attatgcttc aaagtactta atatatatat
     1141 tccttacaac agctatagtt catggtggtc ttggaataga taaaggacaa gctgctataa
     1201 tgcaagccaa ccttgttgca ttcacatacc ttgcacctat aattggggga tatatatcag
     1261 atagatggat aggagctaga tatactatac ctataggtat gcttatcatg ggagtaggat
     1321 actatcttgg ttctattgca actacagttt ctatggtaaa tgcaatgata atcttagttt
     1381 caattggtac tgcattcttt aaaggtaacg tatctgcggt aaatggacag ctttttgata
     1441 gtcaagaaga acttgataca gcattttcag tacagtattc ttttgttaat ataggttctt
     1501 ttattggtac tatagcagtt ggtattcttt accttaaaac atttgcaaaa aatggtgtac
     1561 ttggattttc tcaatgtttc tttatagcag ctgtactttg tgtaataggt gcaatatggt
     1621 tcatatatgg atggagattc cttgggaatg caggaaagag accttttaaa gaaggtgtag
     1681 ttgctgaaaa aattgaagaa aaagataagt ctcctcttac aagcatggat aagaagagaa
     1741 tttgggctat tatacttata tcattcttct ctgtaatttt ctgggtattc tggtatctaa
     1801 cttatcttgc agtttatgat tatggtgcag catttgtaaa catgaatgtt ggaggatttg
     1861 atgttcctct tgcttggttt gactcattaa attcacttgt atgtatagta ttaggaccag
     1921 ttcttggagc attatggttt aaacttgcta gtagaccaca aggagatatg agtttattta
     1981 aaaagacagg tcttggatta atattcttag gtcttgcatt cttaatgctt gttggtgcag
     2041 aattttcaag aggggttgga gctcctgaaa ctgctaaagc tagtatacta tggataatca
     2101 tgtttggtat tcttttaagt cttggtgaaa tgttattctc accacttggt aactcttttg
     2161 taagtaagta tgcacctaag aagcttctag gtgttcttat gggagtatgg acatttgcta
     2221 cttttatagc tggtaaagga tacggatata tatatgcatt tactcttaaa tttgatatga
     2281 ttaaagtata tactataatt ccagtaatac tatttgttgc agcgatatta ttattcttat
     2341 ctgataagac attaagtaga cttgttgagg atgataaata attagtaagc aattattatg
     2401 ttttataggt taatctactg taattatgtt gtattagatt gatttataac taattaataa
     2461 taaaaataaa attttacttc taaatacaat attatgatat atataattat aatatttgtt
     2521 agagtatgat tattaaataa aaataacaaa taaaaatact agctttagtt agctagtatt
     2581 tttatttgtt tttattaggt atgggattaa tacataattt caccacttaa aatagtataa
     2641 gcacttcctc caatttttac agaagttatg tcattgtttt ctctagttat atttacatat
     2701 acctttccag gtctgtttat aaagttacct tgctcaccaa taaaactagt tttatctaaa
     2761 atattgtgat gtactatata agatgatacg caagcgttcc cagttccaca tacagggtct
     2821 tctgtaactc caacatatgg tgctatagat cttaaatggt agtcacactt tttatctata
     2881 gtttctatac aaaatggagt tataccaatt atattattat caccactaaa ttttttgatt
     2941 ttatttaaat ctggagagat attttttaaa tcatctaaag attttagacc aaatacaagc
     3001 caccaaagtc cagtatttac ctttataata ggatattcaa gaagtctatc ttcatgcaca
     3061 cctatcattt gtgcaagttc ttttctgcaa ttatctaaat tttcaaattc aggtgtattc
     3121 tgttccatca taaatatatt ttcattattt attttcttaa gctctacaga taatacacca
     3181 gcttttgttt cttgataaaa tatactctta ttagtattaa gcttcccttc ttccaaaagt
     3241 gcatggaaag tacctattgt tgcatgacca cataaatcaa cttctaaacc tggtgtgaaa
     3301 aattgtactt taaaatctgc aatatttgac tctgaaacaa atgcagtttc tgataaattc
     3361 atttctttag ccacattctg cttaatttca tcagaaatgt tagaggcatc agtaacgaca
     3421 ccagcaggat ttccaccaaa aggtatagtt gtaaaagcat caacttgttt aatcttaatt
     3481 ttgttcattt ttataacctc cttttatttg ttctgaaata taaatttcat gttttatgta
     3541 ttgaactcca atatataaaa tgttagagtt ttttgtccaa attgaaatgg cctcactgcc
     3601 ttctgataag tttcctgtca atatttcatc attatctaca atcaaaataa ttcctttgta
     3661 aagcatttta ttttcaagtg tagttttatt acctccatga atatgaatat tatcaagacc
     3721 tttcagactt ttgttttcta aaagtagtat ttcaatatta atattttttt tgtaagcaat
     3781 taggagttct ttttttaatt caagaatttc ttcataaggg caagaaatta taatttcttg
     3841 ttttgcattt tttatcattt ctattgcttt ttttaatata tttttatctc cttttatgtt
     3901 taatacgtat tctacacttt tacttttatt taatatctgt aagctattct ttagattatc
     3961 catagaattg tcaaattctt ttttcttatt ttttaataat tcattagggt ttattggtat
     4021 gtacttaata gggtcagttc caacattgat aattatatta ttgtttaata gctttgttat
     4081 attttcatag acttttgatt gtggtacacc tgataattta cttatttcat atcctgttaa
     4141 agggctttcc tttaataatg atatgtatat ttgagattcg tatttagtaa atcctatatt
     4201 ttgtaattca ttaattatat tatccatata gaaactcctt tgataaaaga ttagtaacta
     4261 cctatgtagt tattaatttg attttataat ttaagggaaa aaatgtcaac tatattaaga
     4321 aaaaaataaa atatattaga aatatttagt ctcataaata tttaatctta taaatctatt
     4381 tatcataata ttaaatctat taaaaatagg cggttacaaa aaagtgccta attgaactgt
     4441 tatatagggc gtagtatatt aatatcaaga agataataaa ggagatgtat attatggtaa
     4501 aaataataaa taataatgaa tttataaatg aagtagaaaa taaagatggt ttagtagttg
     4561 tagatttctt tgcgacttgg tgtggacctt gtaagatgct atcaccaata tatgaagctt
     4621 taggaaatga gatggttgaa aaagcaaact tcttgaaggt ggacatagac caaagtatag
     4681 aattagctca gaaatttgaa gtatctacag taccaactat gctaatattt aaggatggaa
     4741 aacctgtaga tagattaata ggttttatgc ctaaggagaa tttaaaaaac aaaattgaat
     4801 catatatgta attaaagtaa attgaagtaa ttaaaataaa tctcatatat aaacaaaaaa
     4861 actcattgta atgagttttt tgtttgtata gtgtaaaata gtttgtatag ttacaaatga
     4921 gtgtctaatt gctaatgaaa ttgactgttg atataatttg tttttttgtg aaaacaaaac
     4981 tactctctat ttattgataa aaaagaatag aaaaatctta tttaaaagcc aaagttttaa
     5041 tagcatgtat aaagctatcc acctcatcaa aagtattgta cattcctaag cttatcctaa
     5101 caagacctac aggattttcc ctcataaatc tgacgtatgc ctcttttgga tttataccta
     5161 ataatttttg tacataaata tctgcaccta ctactccagc tccagtttct attccaaagt
     5221 tatcaactaa atattttgct acatctttgt aatacatatt acttatatta aaagatatat
     5281 ttgggataat tacattattt gatttatctc catatattat tatattaggt attttactca
     5341 tttcttcttt agcatataaa aataattctc tttcgtattt tgaaattctg tttagaccaa
     5401 ttttatttaa agtgttcagt gcttttccca ttgcaagagt tccaaaatac tctgggtatc
     5461 ctgcttcaaa acgttggggt acgtttgcat atagtatttt gttatcattt acaaatttgg
     5521 tagaacctga gccatatagt acaggattaa atttttcaaa gaatttttta ggtcctacca
     5581 gagctcctcc atttagtgga gaataacatt tatgagcact aaaaacaaca aaatctatat
     5641 gctcatcaca ttcatgtggc atcattgaaa aaggtttatg ctgaattact tgcacaatat
     5701 ccaccaatat ctttgcacca tacttatgag ctattcttgc tatttcatat ataggagtgg
     5761 ttatacctgt gacattacta gctcctgtaa ctgtaacgag agatacatta ccagcataat
     5821 ttttaagtat attctctaat tgacataaat caagttctcc tttttctgta attccaacaa
     5881 cttttgtagg aaaattaact ttaaaaggca ggtaattagc catatgctcc attgctgtgg
     5941 taataacaaa tttatctgga ttttcatctt taaaaaattt tgctaagaag tttattcctt
     6001 ctgtagcatt tttgacataa ataacagaat ccttttcttc gtctccatca ataagtttga
     6061 ggatagtaga gcgtacattt tcatatttta aagtattttt ttctgagatt attcctggag
     6121 cattaatata agtaagccaa ggaatctcac agtttaagtt atccactacg ttttttaaaa
     6181 ctaatggagt ggcagagttg ttaaaatata ttcttttaac agtttcacca tttttagttt
     6241 taactttaag gtttgcacct tcaatgtatt tagatacatc catgaattgc ataaaaaaac
     6301 cccttatcta ataaactatt ctgtttttta ttaaaaaagc tcaagtttta atcaagagct
     6361 tgtttaatat atgttatgtt tttataaggg gttttgttaa ttaattcttg ttgagatgtt
     6421 tattaaatta cacgaaaatg agctacagtt gggggaaact gtagctcact tgattttggg
     6481 ggagataggt tgaacttatt gtttatccgt atcatatttt tagtatagtc acttatttta
     6541 atttatatac atttttttat aaattttaca taaaatgtta aacactaatt agaaataatt
     6601 atgaatatga ctaataaagt tgtaaaaata gtaaagaaat tgattagaac ataatggaaa
     6661 atatattgta tataattaga aaaaaagatt ttatatgtta taaaagatgt acaagtataa
     6721 taattttata aaaaatacaa aatataatac aaataatttt ataaaaaaaa ttataaaaat
     6781 ttttaattta atttatataa attaaaaatt atattaccaa tgtatatatt tgaaaaaaga
     6841 ttagtacaaa actataatct taatttcaat aaatatatat ataattaagt tgaaatactt
     6901 gaaaatactg gaaaaaattg aaaataattt ttgtaatata aaatgtcgat tgattttaaa
     6961 taattaaata tacataagaa ataattaaat atctttcaat gaaaatatga taaaatatga
     7021 caaattaatt gatgacaata aaaaatattt tgttattgta gtattatagg gtaaattgca
     7081 aaatatatac gaaagtaaaa ttttaaatat tgacattaaa ttaaatatac tatactatgt
     7141 aagtgtgatg agagaatttt tttagagggg atttatagtg aatataaaaa agatatttaa
     7201 taacaatgta gtagtaagct ccctagaaga tggcacagaa ataattgtca ctggtgctgg
     7261 agtaggattt aagaaaaaag taggcgattt gatagatgaa aatttgattt ctaagaaata
     7321 ttttgttcaa gacgatcaaa gggacaaata caatcaaata cttaataaga cctctattga
     7381 atactttaaa atttcagaag aaattataga aaaggctaat gaagttttaa atactcaagt
     7441 gaatgattca ataatattag ccttaactag tcacatagaa tttgctgtcc aaagagaaaa
     7501 acaaggtata aaattaccaa acttaatatt gaatgagaca aaacaacttt atagggaaga
     7561 atttgaattt ggattatggg caattgatga aattgaaaaa aggataggaa taaaattgcc
     7621 tgaagatgaa gcaggatata ttgctattca cataatcaat ggtttggaaa attctcaaaa
     7681 agatgaaggt attaatatac tagagttttc aaaacaagtt attcagataa ttgaagaagt
     7741 acatggattt aaattggatg taaattcatt aaattatact agacttataa ctcacttaaa
     7801 gttttttgtt caaaggattc tccgaaaaga aacttacaat gatagtgata ttgaagatat
     7861 gtataagtta gtgaataaaa attacaataa acctaaagtg tgtactgaag aaattgcagc
     7921 attggtatta gacagatttg aatataagat tagtaaagaa gaagaattat atttaatgat
     7981 tcacataaat aaaataacaa acagtggttg ttaggattgt aactgataaa gcaggcaaaa
     8041 cctaagtcat tgagcttaac tattagatta agttttttga tttaggtttt tttattagat
     8101 acaatcaaaa tacagacagt gacttttagg attgtaactg gttaagcagg caaaacctaa
     8161 atcattgaat ttaactatta ggttaagttt tttgatttag gtttttttat tagatacaat
     8221 caaatacaga cagtgacttt taggattgta actggttgag caggcaaaac ctaaatcatt
     8281 aaacttaatt attaaattaa gttttttgat ttaggttttt ctataaattt gattatgttt
     8341 ttagataaaa ataggagggg aaaattatga aaaaaaataa gttaggttta tggaacttct
     8401 tccaaatgtt aggaaaaacg tttatgttcc caatagcact acttagtgtt tctgggatga
     8461 tgctaggact tggggcaggt tttacagacc ctaagatgat tgaaatggtt ccatttttag
     8521 ggaatgaata tgttaactta gttttaaact tcatgttaac aataggatta tttgcattta
     8581 acaacttggg agcattattt gcaatggcaa ttccactagg attattaaaa aaagaaaaag
     8641 agtttggtgc tttttcagga ttagtaggat ttatagcaat gcatataggt acaaattttt
     8701 atttaacaag agctgatttg ttagtaccag cagaacagat gtcaactaat ggtcaagcta
     8761 tgattatggg tatacaaaca tataatacaa gtgttttagg tggtattgta gcaggattaa
     8821 ttgtttatgc aatatatgat aaagttagta atttaaagat accagaatca ttaggattct
     8881 acagtggacc aagattagtt ccaattgtta gtttaatagt tatgagtata gttggattat
     8941 taatacctat tgtttggcca ccatttttca atgcattcca aagtttagga agatggattt
     9001 cttcagcagg accattagga tatttttcat atgcagtggc agaaagagtt acaattccat
     9061 ttggactaaa tcatcttgta acatctgtat tccgttttac accaataggt ggaactgctg
     9121 ttattgatgg agaaacgtac tttggtacat tgaacatgtt tatggcatat gtagaaaata
     9181 atcagattat acctcttgat ttagctggta aaatggaaca aggtaaattg atgattcaat
     9241 atggattagc tggtgctgca ttggctatct accgtacagc aaaaccagaa aatagaaagg
     9301 cacttaaagg acttttaata tcaggagtgt tgacagttat tataggtggt ataagtgaac
     9361 caatagagtt tctattctta ttcatatcac cagcattatt tgctttccat acatttatga
     9421 atggtatagc aaacatggta cttccatact taggcgtgtt gatgggattc acaggagatt
     9481 taattgcatt tataagtttt ggtgtgttaa gaggaacagc tacaggatgg ccaatagcag
     9541 tattagtagc agttttatat tttgcaatat actattttgt atttaagtgg gcaattatta
     9601 agtttaatat aaagacccca ggacgtgagg ataaagaaat aaaagtatct gaaggaaatg
     9661 gttcagattt caaaaatttg tctacatata aaggacaaca aatgatatct gctttaggtg
     9721 gtcaatctaa tatattatca ctagataatt gtgtaactag attgcgttta aaactacaag
     9781 atgtatcatt aataaatgag gatgcaatta aagaggctgg tggtatagca gttgttaaat
     9841 tagatgagca tacaatacaa gtaattatag gaactcaagt gtattcttta agaaagcaga
     9901 tggataaagc tatggaaggt catttggagg catgtaatga ttaattttga taaaaaagta
     9961 gaccgattgg gtacatattg tacccagtgg gactatgtag aagatagatt tggtaagaag
    10021 gatttgcttc cttttacaat ttcagatatg gatttagagt ttccatttga aataacagag
    10081 gtgttgcagg aaaggttaaa tcatagagtt ttgggataca gtagatggaa acatgatgat
    10141 tttagaaatt cagttacgaa ttggtatatt aaaagattta attgtagcgt tgatagtaac
    10201 tggatatatt atagtccaag tgtaatgtat tcaattagca aacttattga gatgttaagt
    10261 aatgaaggag atggaatcct tataaatact cctgcataca atgcttttta tgatgtgata
    10321 ataaataaca atcgtaatat tataaagtca catcttgtaa ataggaatgg attctattca
    10381 attgatttta atgattttga gaaaaagtgt aaaaaatcta agatatttat tttatgtaat
    10441 cctcacaatc caactggtag agtatggagt gagttagaac ttgttaagat gataggaatt
    10501 tgtaagaata atgatgtcaa gataatatca gatgatatac acatggatat tgtatatgac
    10561 aatagaatga cacctatctt aaatgttgca aaggactatt tagattctgt attcatttgt
    10621 acatcagcgt ctaaatcttt caatatacca gcattgactg ggtcatatgt gattatacca
    10681 aatgaaggta ttagaaataa atttgaaaat atcactagat accgtgattt tgtaaattcg
    10741 ccagcaattc tagcaatatt agctacaatg gtctgttatg atgactgcga atattggtta
    10801 gatgaactat taaaatacct taaagagaat cttaaatata cgattgatta tataaaagat
    10861 aatttagaac cattgaaatt ggtcatgcca gaaggctgtt actttgcttg gatagatttt
    10921 tccgaactta aaataagtgg tgagaaattc caaaaattat tgatggatat tggagaagtt
    10981 gctattatgt cggggtctgt atatggtgaa gaaggtgagt tttatttaag gttaaatgtg
    11041 gcatgttcaa gagaaaaact tgaagatggt ttaaatagga ttaaaaaaac tatggattat
    11101 attgcaaaaa gaattttata gtaggaaggg agatagttat gggatattta aaaacaatgg
    11161 ttgatgttat gactactgaa atgaatgcca taagatgttt aatagatgaa gcaggaatag
    11221 aatatgaaag tatagttagt gaaattgcaa attgtgaagg caaagtaatt tttatgggtg
    11281 taggaaaatc tgcacatata ggtaagaaat tggcagctac atttgctagt actggtacac
    11341 ctagcttttt tgtacatgca acagaggcag tgcatggaga tttaggaatg attgaaagta
    11401 aggatattac aattttaata tcaaatagtg gaaattctat ggaagtggta aactgtataa
    11461 aatatattaa agcaataggt tctaagacaa ttgcatttac ttcaaacaga aattcagtac
    11521 tggcaaaaga gtgtgattat gctttaatat atcctgcaaa ggatgaagca gaccatttaa
    11581 atctagcacc aacaacatca tctacaatta ctttggtttt aggagattct attgcatgtg
    11641 cattatctaa gagtagtaat tttggttctt cagattttta taagtaccat ccaggaggaa
    11701 gtttaggtga gaagttaaaa acagctaaca atggataaaa gcaaaaagga gagtagtttt
    11761 tatgttcaaa atgtttaaaa aaaaatcaat aaaatcaccc atatcaggaa aagtaataga
    11821 gttatctaaa atacaagatg gtgtattttc tcaaaaaatt atgggagagg gtgtagctat
    11881 agattctaca ggagacattg tatatgctcc aatcaatgga aagatagtag tagtagcaga
    11941 gactaaacat gcatttgtaa tagaattaga aaatgggatg gaacttctta ttcatgttgg
    12001 tttagatact gtaaatttaa aaggcgaagg ttttgaggta ttggtgagta tgggagaaca
    12061 agtgaaggaa ggaacaccaa tgttgaaaat taaccgtcct ttgattgaat caaagggtat
    12121 atcacttatt actccagtta caataactaa tcattcagag tacaatatga atacatgtaa
    12181 tgtaggcaat agtgttgaag gtgggaaaga tactgtaatt gagtttaaat aaagaactaa
    12241 aatacaaggt taatatttat aaatacctat aaacttatgt tttataagat tgattgtata
    12301 gatttgttgt atagatttgt tgtataaaaa agaactcttt aattataaat taaggagttc
    12361 ttttttattt tgtaaatatt gtttagattt atatagaaaa acact
//
LOCUS       DAEMGG010000117        12072 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEMGG010000117 DAEMGG010000000
VERSION     DAEMGG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.118"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            190..522
                     /locus_tag="KPK24_003001"
     CDS             190..522
                     /locus_tag="KPK24_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG2300653.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            684..869
                     /locus_tag="KPK24_003002"
     CDS             684..869
                     /locus_tag="KPK24_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300654.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            896..1981
                     /locus_tag="KPK24_003003"
     CDS             896..1981
                     /locus_tag="KPK24_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG2300655.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            1974..2432
                     /locus_tag="KPK24_003004"
     CDS             1974..2432
                     /locus_tag="KPK24_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300656.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            2467..3558
                     /locus_tag="KPK24_003005"
     CDS             2467..3558
                     /locus_tag="KPK24_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2300657.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            3563..3787
                     /locus_tag="KPK24_003006"
     CDS             3563..3787
                     /locus_tag="KPK24_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300658.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            3797..4360
                     /locus_tag="KPK24_003007"
     CDS             3797..4360
                     /locus_tag="KPK24_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300659.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            4321..4950
                     /locus_tag="KPK24_003008"
     CDS             4321..4950
                     /locus_tag="KPK24_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300660.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            4990..5127
                     /locus_tag="KPK24_003009"
     CDS             4990..5127
                     /locus_tag="KPK24_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2300661.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            5127..5879
                     /locus_tag="KPK24_003010"
     CDS             5127..5879
                     /locus_tag="KPK24_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300662.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            5892..6377
                     /locus_tag="KPK24_003011"
     CDS             5892..6377
                     /locus_tag="KPK24_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300663.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            6384..6533
                     /locus_tag="KPK24_003012"
     CDS             6384..6533
                     /locus_tag="KPK24_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300664.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(6670..7491)
                     /gene="yidA"
                     /locus_tag="KPK24_003013"
     CDS             complement(6670..7491)
                     /gene="yidA"
                     /locus_tag="KPK24_003013"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG2300665.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            7984..9678
                     /locus_tag="KPK24_003014"
     CDS             7984..9678
                     /locus_tag="KPK24_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG2300666.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      10010..10120
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10224..10595
                     /locus_tag="KPK24_003015"
     CDS             10224..10595
                     /locus_tag="KPK24_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG2300667.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            10692..11639
                     /gene="trxB"
                     /locus_tag="KPK24_003016"
     CDS             10692..11639
                     /gene="trxB"
                     /locus_tag="KPK24_003016"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG2300668.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            11743..12060
                     /locus_tag="KPK24_003017"
     CDS             11743..12060
                     /locus_tag="KPK24_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG2300669.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         4787 a         1254 c         2149 g         3882 t
ORIGIN      
        1 tattttttat tcattaaaat taataaaaat agaaaaaaat caatcaataa tttaataaaa
       61 atagataaat actatataca cttttaataa tatagttaat atctatttta cagataaata
      121 agaataaact tataatataa ttgtatctgt aaaattttgt aaaatataaa aagtataggg
      181 ggaataaaaa tgaaagtagc ttatattttt agtagtacta atacacataa aattctagac
      241 aaaatgataa taccacaact tgaacaagga actcatggag tagacgttct agggatgttt
      301 ttctttatgg ataatacctt atttttatct aaaggtaatc ctgtaggcga gagactaagt
      361 aaattacatg aaaaaactga tatgataata atggcttgtg accaatgtgc tatagaaaga
      421 ggtatagaga ataatttggt tgatggagct acaattggat gtttcccaaa cctatatgct
      481 tgtttaggca gtgcaggtgt ggaccaagtt ataacgttat aaattagtat aggatacaat
      541 ccatagaaaa atttattttc tgtggattat tttaagtaaa agataaattg tttaaagtag
      601 gggatgaaca aatgtctaaa attttagttg aatttataaa cacttgacca tcttgtgatg
      661 aacatggagt gtttataaaa aaaatgtcag aaaagtatgc tgataaattg gaaataaaat
      721 tatatcaagc agggaaagat ttttcgtata taaaaaaata tgggattata acaaaaggaa
      781 ctttaataat aaatcagaaa aagaaatatg atagattaaa taaagataca attgaaaggg
      841 caatagtaga agctataaac aataattaaa agtttaaaaa ttaggagtat caaaaatgaa
      901 tatattagaa attgttttaa atggagttaa agatactata gtagcttcta tagttacact
      961 gaatggagca tcaggatggc ttatgataag ttttatcttg gcaggtgtac ttcacaatgt
     1021 tttaactcca agaaggtttc aaaaacaact tggaaataag aaattttctt ctttaattaa
     1081 aagtactatc tcaggaatgt ttttaccaat atgtagttgt ggagtaatac cacttggaat
     1141 aagtatgtat tattcaggag catatttagg accagtactt gcttttatga catcaactcc
     1201 aataataaat ccaatagctg tagttctttg tctaggtctt ttaggtccta aagttacaat
     1261 catttatcta atagctggtt ttttagtacc atttttagta ggatttttag gaaattgttt
     1321 tggaaaagaa gaattatcac ttcataatga ttcagaggaa gaagtaatag aattagaaga
     1381 agaaaaaaga agttttatgg aaaatatgat tgatggtctt aaatggtctt ttggtgaatt
     1441 agcccttact attagtaaat atgtagtaat gggtatggtt tttgcaggat ttataacaac
     1501 tatttttcca aatagtataa ttcaaaaata tttaggaaat ccaggtttgc tatctcttgg
     1561 aagtatagca atacttgcat gtgttatgta tgtatgtgct gtaggacata taccgtttat
     1621 agcagccctt gttgcaagtg gagcatctcc tggtatagcg ataacatttc taatagctgg
     1681 agcagctaca aatctaccag aacttataag tatgtttaaa cttattggaa aaagaactgt
     1741 tattatatat tctgtaactt tgacactgtc ttcaataata attggatata taacaaattt
     1801 acttttagtg aataataact cagtaaattt agatgcaaca aattcttcaa tacagttggc
     1861 aaataaacta atgttaaata tacctgatag ttttgagtat gtatgtacta ttttaattat
     1921 tttatttgca tttaaatcaa taataccaaa gatgaaagag gtcataggaa atcatgctta
     1981 aaaagatagt tataatatcc tgcatagtag ttgtgttaat cattttatca aaaatagttg
     2041 atgataacat caaagaagat gcttctattc caaatgtgaa taaagaaaca ttagaatatt
     2101 ttagaaaaaa ttataaagaa gacataataa cttgtgctga agaagactta aataatgatg
     2161 ggaaaaaaga tttagtagtt atatataaaa aaagcaataa ttcaaatgaa atggtagtcg
     2221 tagtttctga taaaaatagc cactatataa caaagccaat accagcacct atagagaatc
     2281 aaactataac ctttaaaaat attgatgata aagcaccaat agaagtaatt gtatcagggt
     2341 ctaaaaatgg taatgtagga tatgctattt acagagtaga gggtaaaaaa tttgttgatt
     2401 tatttggcga agatatggac aaatgttgct aaaaataaaa ataatacata aaggtgggaa
     2461 atttatatga aattatctaa gaagattcaa gcattaatct tatttggtgt aatgggtaca
     2521 tctatattaa caggatgtag tagtaaacca aaagaaaaag aagaagcaaa aactggaaaa
     2581 gatgattata caataacagt aggttattat aattgtgacc atatgactgc gggaccagta
     2641 gcagaatcgg caggaatata taaagattta ggtcttaatg taaaaactgt tggaaatggt
     2701 aaagttcctg aagctatggc agcaggaaaa atggatgcag gttacatagg aactaagggg
     2761 ttagttggag caatacctaa gggttcacca ataacaatag cagctaacaa ccatactggt
     2821 ggttctgaat atttaatagt ttcaaaagat ataaaagaac ctaaagattt aataggtaaa
     2881 aaaattgcaa cagatatgag tgatttctta tggacaagtg attatggtcc agaaacaggt
     2941 ctaccaactg acccatcaaa atatgaagtt gtaaatatgg attcagacaa agataaatat
     3001 ttagctctta aaactgggaa aattcaagct tttacatctt gtgacccttg gggttctgta
     3061 gctgaaaatg acggtgcagg aaaaataata gcatctactc aatataaaga aaaagctaat
     3121 ggcaaagaat ataactgttg ttcattctca ttaaacaaaa actttataaa agaacatcca
     3181 gatttagcaa aaaaattggt tctagcacat acaaaagcaa tagaatatat atatacaaat
     3241 ccagctgaag cagctaaaat atttgcaaaa tattataatg tagaagaaga agttgcactt
     3301 agaactatct ataagaagac tgttggagaa ggtagaactc ttacttggaa agttacaggt
     3361 gaagaatata aaaataattt acaaatgtat aaagatttaa aggctttaga tgatatacct
     3421 aaatatgaag atacgattga tacaagctta ttggactctt gtggagctga tgattttgac
     3481 aaatttataa aagaaaaagt taacccagag tttcctgaag gtatgagtta tgaagactgg
     3541 aaagcaaagg tattataaat aaatggagaa tatgaaaaat ataataatac tttttatcat
     3601 tattttggga agtatattta taatattttt taattttgaa gataaagaag aaataaaagg
     3661 aaaattaaga ataggggtat ctgatgatac ctctggtttt gtaataaatt atatggtaaa
     3721 tagagactac tttgaaaata taaaagtaag tgatataatg gatattttta caattaatga
     3781 ttgctgagca agtacaatgc agtgggctct gagttcagag gaaatagata tagcaatcat
     3841 atgtaaagat gctgcaaaac aatatgtaaa tgtaaatagt ggatttgaaa ttgttggtac
     3901 tgtagttcaa aattctgata tatttctatt aagtgataat aatcctgaaa agataggagt
     3961 cattcaaaat agagattacc agtcagaatt ggttaaaaaa tactacaaga atgtggaagt
     4021 agctccctta ttagggactg cactagcata tggattagaa agtaatttag tagatggtgt
     4081 tgtaatagat tgcataaagt caattgggtt agagggcaaa agaaaatcta caactaaatt
     4141 aggagattat gatacctatg tattagttgt aagtaaagag tttaaaaaaa gtaaagcata
     4201 tagtgatttt ataacacttt acaataaaag tgttgatgct ttaaagaata aagctacatt
     4261 taagaaagca ttaaaagagt acaggaatat aagaaatata gatatggagg aaataagcaa
     4321 ttggaaatta aaatttctaa agataaaagc gatagattaa ctaagaaaaa aactgatggt
     4381 agtatggata ttttatataa aataatacta ttagttatta tatttttagt ctggcatttt
     4441 gcagcaaaag atatagggag ttctttgtta cttccaatgc cagtagatgt aataaaaggt
     4501 ttctttttct gtgtgacaga tgcagagaca gtcactaatc tttttataac attgcagaga
     4561 gtacttaaag gttttatgta tgcattattg tttgggcttc caataggatt tattatggga
     4621 ttttctaaaa cttttgaaag ggttttatct ccagtagtag attctgtaag gcaagttcca
     4681 ataatggcat gggttcctct tactatagta tggtttggta taggagatgg tccaacaata
     4741 tttttaatag ctttttcagg ggttttccct ataattttaa atactattca aggagtaaga
     4801 gcaatatcaa aggattatta taatgcagct agaagtatgg gagcatcgcc aattgtgata
     4861 tttacaaatg taattgttcc cgcatctctt ccagatatac taactggttc aaggattgct
     4921 ataagtacag gatggatgtc tgttatctga gccgagttta tagctacgag tgccggtctc
     4981 ggttactcaa tggtacaagc tcaaacatta atgcatacag atgtattaat tgggcttatg
     5041 atatttgcag ctttaatagg atttataata gatagggtat taaagtttat aaataaaatc
     5101 ctttgtagat ggaggtttgc agattaatga agttaagtgt aagagatata aataagacat
     5161 ttgtaaataa tagagtacat acaaaggttt tagaagatat aagtatggat atagatgatg
     5221 gacaatttgt ctgtctttta ggaccatcag gttgtggaaa aacaacttta cttactataa
     5281 taggtgggtt tcaaaaatca gaacgtggag atgtttttat aaatgataaa agagttaaaa
     5341 aaccgggcat agatagagct tttatatttc aaaattatgc tttatttcct tggaaaacta
     5401 taagaggtaa tgttctattt cctatgaaac agcagaaaat accaaaagaa aaaagagaag
     5461 aaatgctaga agaacttttg gttatgtcag acttgaaagg aaaagaaaaa ctgtttccgc
     5521 atcaattatc tggaggaatg aaacaaagag tggccatgat aagagcttta gcatgtaatc
     5581 cagaggtatt actaatggat gaaccattag gagctgtaga ttttcaaatg agacaaaatt
     5641 tacaggaaga gctagaacga atatggataa agaagaaaat aactgcatta atggtaactc
     5701 atgatgttga tgaagctgta tatatgagtg atagagttat agtaatgtcg agagataaag
     5761 gaagaattat tgatgatata aatatagata ttccaagacc tagaattaga ggaagccaaa
     5821 aatatgaaga gtacaaaaac aaattaactg atactttatc aaagtgttat gaagtttaaa
     5881 gaggagtaga catgagtcaa atagaaaaat acaatagttt ggataataca caacagaaaa
     5941 taagaatctc tattatggat atgataatag ataaaggttc tagtgttaca cttcaagaag
     6001 tgactgaata tataagtaaa aagttgaata tagaaaaaga atatatagaa aggacactac
     6061 aatattttat ttataaaaat ataatggttg ttgatgggaa tagtataaat tttatatatc
     6121 ctgtatcagc attgccaaca aatcataaag taactttgag agataataga agtttcagtg
     6181 caatgtgtgc tatagatgct ataggaacct cttgcacatt caaccaagat ataaaaataa
     6241 actctatttg tagtgctaca ggtaaggaaa tagaagtaat tataaaaaat gaaaagattg
     6301 agtatgtgaa taatccaaac ttaagagtgc ttcatataaa tcttgataag catctaaatt
     6361 gggctgctag ttgctgaaat ataatgaatt tcttctggac gaagagtgat tttgatgctt
     6421 ggacaaacga agcgggatta tctaatgatg aagatatata ttgtttagat attaatgagg
     6481 caatagtaga atcatataaa atatttaaac tcaaacaaaa ggtactatca tagatataca
     6541 tatatgaaaa actaaaaatg acctttagat aaaaatgata ggtaatatcc atactatata
     6601 aattagtata gagtagggat gtgaaaagta tttaaatcta aaggtatatt tttagttttt
     6661 tttagatgat tagatagcct tattaagtat atatttattt ataacctttg caactccatt
     6721 ttcattatta gtatcagtaa cataatctgc aatttccttt atactattac gtgcattccc
     6781 catagccaca ccaagacctg catactttat catatgttgg tcattttctt catcgccaac
     6841 agcaatagca ttttctatag gtatatttag attcgtacat aatgttttaa gagcagttcc
     6901 tttattagta gttttaccta aaaattctag ataaaaagga gcagacctta ctatagtata
     6961 tttattgaat aaatttttag gtagttgctg tatgatttct tccaatcttt ctggttcatc
     7021 aactatcata attttacaga ttttaataga ttcatcaatt ttatcttttt gtatatattt
     7081 gacttctata ttatttaatt tcgcttcatg aagtgtatat ttgccaggcg tttcatttgg
     7141 tgtaattaaa tttgtcattg tatttatgtg tattttagtc ttatattttt tacaaacgtt
     7201 attatatata aaatcaaaat catctaaact catttctaca tcatgtatga ttttctcact
     7261 tttaacttct tgaataattg ctccattaaa gcatataaca taatcacctt catctgttaa
     7321 atttagttga tttagaagtt cttttacacc aggaagaggt ctgcctgttg ttataactac
     7381 ttttgcccca gccctttttg cctcctgaat ggactcaaag acttcaggag taatcctttt
     7441 ttgtgtattt aatattgttc catcaatgtc tagtgcgatt aatttgtaca tatcaacctc
     7501 caataaatta tatactcaaa tttcctgtga aaacgttgac acagctagtg ttgaacaaag
     7561 tttcagtggg aaatatggtt tgttttgtat actattatat caagagataa gtaatttaga
     7621 aagtttaata atattaattt tttgataaaa atattactta tgtgagatga gaatgtaaaa
     7681 actttgtaat caataattcc aaaattctta attacaaata atggtatcgt ttgcattgtt
     7741 aatataaata aatgtatatt atatagttag aagtttgaat aaatagactg atggtaattt
     7801 gttatattag ttagttttgt gttataaaat attgtaattc cattaatcat atgttattat
     7861 tcaacatgat tataatataa agaatatttg atatatataa taatttataa aagagtatta
     7921 catagtacat gtaaatctat atttataaat acaataaagt aaagttgtaa aggagtgctt
     7981 taaatgaagt tttcagagtt taagtatgaa agacccaatt ataatagtat gaaaaaagag
     8041 tttttgagtt gtgtagaaga tataaataat tctcgtaatt ataaagagca acaaaaaaat
     8101 atacataaga ttaatttatt aagaaataaa atagaaacgt tgtcaaatat agcttcgata
     8161 agatatagta cagacacatt taataaattc tacaaagaag aaaaaaatta ttgggatgaa
     8221 tatatgcctt tgtatgaaga attaaattca tatttttata atgccattgt aaattcaaaa
     8281 tttaaatatg atttgataaa agagtttgga gagcaatttt tcacaatagt agagtattct
     8341 ttaaaaagtt tttccaaaga aataataagt gaattgcagg aagaaaataa attgtgttca
     8401 gagtatacta gacttttagc ttcagctgag ataatgtttg atgggaaaat aagaaattta
     8461 tctggtatgg ggaaattcat gtattctaag aatagaaaaa caagagaatt agcaaacaaa
     8521 gcatattata atttctttga agaaaatgaa actaaatttg atgacatatt tgataaattg
     8581 gttaaactta gagataagat gtctaaaaaa ttaggttttg aagattttgt agagcttggg
     8641 tatgttagaa tgatgagaag tgactacaga gaatatatga taaaaaatgt tagaaaacaa
     8701 gtactaaaat atgttgttcc aatggcaaat gagctttatg aaaaacaagc taaaagaatt
     8761 ggtctagaat atttaagtta catagatgaa ggcgtggaat ttttaacagg aaatgcaagt
     8821 ttaaagggag attcaagata cataattaaa aatggtaaaa ggatgtattc tgaactgtca
     8881 aaagaaacaa atgaattttt tgattttatg ttagaaaatg aacttatgga tttagaaacg
     8941 aagaaaggaa aaggagctgg agggtattgt acttatatac cagattataa gtcaccattc
     9001 attttttcta attttaatca aactgctgat gatatagatg tattaacaca tgaggcagga
     9061 catgcatttc agttgtatat gtctagatgg attgatatgc cagaaattaa ttttccaacg
     9121 cttgatagtt gtgaaatcca ttcaatgagt atggaattta taacatggcc gtggatggat
     9181 ttatttttta aagaagatac agataagtac aaattcactc acttatcatc ttcaattaaa
     9241 tttatacctt atggagtaat tgttgatgaa tttcaacact atatttacaa aaatcctaat
     9301 gtagataagt caaaacgaaa agaaatctgg agatttttag agaaaaaata cttacctcat
     9361 agaaaatatg gtgataattc ttttttagag agaggttgtt ggtggtttaa acaaggacat
     9421 atatttaaaa atccgtttta ttatatagat tatgtattag ctcaaatttg tgcgttacaa
     9481 ttttggaaaa aaatgattca agatagagat gcaggatgga aagattatat aaatatctgt
     9541 aaagttggag gaacaaaatc atttttagat attgtgagta tgggaaattt gtattctcca
     9601 tttgatgatg gctgtattga gtctattata ggtgatgtta aatcttggtt tgatgaaata
     9661 aatgacagca aattataaaa aattatgaga attttattgt ataaaagtga aaaattaaaa
     9721 aatatataaa aagtgattat ttgttgtgaa aatagtcttg tttttaacta aattgataaa
     9781 ataaactaaa aaagatttgt acttaaaaaa aaaagaaaat acaagaaata tgcaatatca
     9841 acatttggaa aaaaaagtaa aaatgtatac aaaatacata taaaaaaact attgcaaatt
     9901 ttatagaatg tttatatact taagtcaaca gagatagaaa aaagctattt atagaaaaca
     9961 aaggataaat tgcttaattt taaattttct gaaaaaaata ataatccgga tgaagagtat
    10021 gggagactgc tttataaaaa gcgaccgaag ggacaagttt aatcttactt acccaaaaga
    10081 ttagatgaaa ttctcaggta aaggtgactg tacttggacg gggctctgaa aatgatttta
    10141 acagggaaga caaattggga gatttgtttt tcctgttttt tatttatcaa gtaataaata
    10201 aaagtttgaa agagagtaaa aaaatgataa taattacgaa taatccaaaa gtaaaagaag
    10261 aagttcaagg cagagaagtt ttgtttaaag acacaactta tattggaatc ttagaagcaa
    10321 gtagagattt gatacatgaa ggttatgaac ttttatctca tccactctat gggagtgtaa
    10381 agccaaatga aacaccttac aggactgtta tactaaagaa aggaaatcgt ttggatataa
    10441 actctttaac cttaattgaa gaagcaatca tcactgcaag taaatttcag aataataaaa
    10501 agactccaaa atggacagaa agtgttcaag atgattttag agtaattgat tatgatatat
    10561 tttataatac aatacaaaga atgcagtatg aataaaaaaa aattgttaaa aagttattaa
    10621 aacagcctaa atattaaatc taatttaata ttaaataaaa tgagaaattt aattggggag
    10681 gataagtcaa aatggaaaat gtgtatgact tagtaataat tggttcagga ccagcagggc
    10741 ttgcagcagg tctttatgga gcaagagcaa agttaaaaac tctaatccta gaaaaagaca
    10801 agactggtgg acaaattgta ataacacatg aaattgcaaa ttatccaggg tcagttccaa
    10861 atgcaacagg accaagttta atagctagaa tggttgaaca gtgtaaagag tttggagcag
    10921 aaatgctaag agacaacatt gttgatactg agctagatgg agatataaaa gttttaaaag
    10981 gggaaaaggc agaatataga gcaaaagcag taataatagg aacaggagca acaccaagaa
    11041 agattggttg tcctggagaa aaagaactta ctggtaaagg ggtttcatat tgtgcaactt
    11101 gtgatgcaga tttctttgaa gattttgaag tatttgttgt aggtggagga gacagtgcat
    11161 tagaagaagc aatgtattta actaaatttg ctagaaaagt aacaattgta catagaagac
    11221 aaggatttag atgtgctaag agtgttgaag aaaaagcaaa agctaatcca aagatagagt
    11281 ttttattaga tacagtaatt gaagaaatca aaggtgatgg aatattagaa tcagttgtat
    11341 ttaaaaataa agttactgga gaaactcatg aatactttgc agatgaagaa gatggaacaa
    11401 tgggagtatt tgtgttcgtt ggattagatg ctcaaactga tttattcaaa ggtaaagttg
    11461 atatggatga aaaaggttac ataataactg atgaagatat gagaactaac ataccaggcg
    11521 tatttgcagc aggagattgt agatctaaga ctctaagaca ggttgtaact gcaactaatg
    11581 atggagcaat tgcatctata gtagctgaga aatacattga tgaaaaattt gggaactaga
    11641 atctgtaaat taagaaatag tgtctgtaaa tttaatgaat aatatttaaa attaaaatta
    11701 atatttaaaa ttaaaataaa aatttcaagg gggaataaga aaatgttaga tttagataag
    11761 gctacatttg aagaagaagt tttaaatgca gaaggttttg tatttgttga tttttggagt
    11821 gaaggttgtg agccttgtaa agctttaatg ccagatgttc ataaattagc tgagacttat
    11881 ggggataaaa taaaattctg taagatggat acaacaaaag ctagaagatt agctataaaa
    11941 caaaaagtat taggtcttcc aactatggct atatacaaag atggagaaaa agtagacgaa
    12001 gttactaagg atgatgctac agttcctaat atagaaaaca tgattaaaaa atatctttaa
    12061 tatgttgata aa
//
LOCUS       DAEMGG010000118        11854 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEMGG010000118 DAEMGG010000000
VERSION     DAEMGG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.119"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(197..1504)
                     /gene="grdB"
                     /locus_tag="KPK24_003018"
     CDS             complement(197..1504)
                     /gene="grdB"
                     /locus_tag="KPK24_003018"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(455..457),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG2300670.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(1590..2876)
                     /locus_tag="KPK24_003019"
     CDS             complement(1590..2876)
                     /locus_tag="KPK24_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG2300671.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            complement(3155..4477)
                     /locus_tag="KPK24_003020"
     CDS             complement(3155..4477)
                     /locus_tag="KPK24_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG2300672.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            complement(4477..6471)
                     /locus_tag="KPK24_003021"
     CDS             complement(4477..6471)
                     /locus_tag="KPK24_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2300673.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            complement(6558..7805)
                     /locus_tag="KPK24_003022"
     CDS             complement(6558..7805)
                     /locus_tag="KPK24_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2300674.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            8033..8578
                     /locus_tag="KPK24_003023"
     CDS             8033..8578
                     /locus_tag="KPK24_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300675.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            8592..9278
                     /locus_tag="KPK24_003024"
     CDS             8592..9278
                     /locus_tag="KPK24_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG2300676.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(9402..10022)
                     /locus_tag="KPK24_003025"
     CDS             complement(9402..10022)
                     /locus_tag="KPK24_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300677.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(10012..11034)
                     /locus_tag="KPK24_003026"
     CDS             complement(10012..11034)
                     /locus_tag="KPK24_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBG2300678.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            complement(11035..11673)
                     /locus_tag="KPK24_003027"
     CDS             complement(11035..11673)
                     /locus_tag="KPK24_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG2300679.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
BASE COUNT         3828 a         2095 c         1233 g         4698 t
ORIGIN      
        1 taatctcgct tttcatagta tatctaattt gaatttgtgt atcaatatta taataaacta
       61 atttatcact tttataaatt taattttaaa aaatgcaaat ttcgaattta cctaataaaa
      121 aaaagatatc gtaaaataat tttaaaaaat tattttttga tatctttttt tatttcagtg
      181 ttgtgttgta tttttattat tcaaatactg tttgttcttc tatatcttct gttagtgcat
      241 ttaaagcttt taaaactata ttctttctaa tttttttact ttcctcttct cctaacatag
      301 gatttccaag tggatatggt atccctactg ctggaacaat tctattagct cctattgtaa
      361 gtgatattgg tactactgta catatatgaa caactgctat accagctctc tcaatctctt
      421 ttaccatcgt tgctccacaa cgagtacaag tacctcatgt agaagttaaa attacagcat
      481 ctacatcatc ttctaacaat tttttagaaa actcttctcc aaatctctta gaattgccaa
      541 cactcgttcc tgttcctgta gtagttataa aataatctac taattctcca attacacctt
      601 ctttttcaag ctctctaact acatctagtg gtacaactaa atttggattt tcaaggacat
      661 atgctctgtc atacccccca tgtatagttg taaattcttt atttgacata gaatccatat
      721 ctgttaaatc gtaaattcca tatttagtag cacttgaaga ttcaattttg tctggattat
      781 caattggaac tatccctcca gaagttacta gggcaagttt tatcttagat aaatctttaa
      841 caggtttcat aggtgtaact ctatcaaatt caggcattgg atactcagtt tcaaagcttt
      901 cccctttcat ctttttaacc aacatatcaa ttgctctttc agaccctcta agttcatgga
      961 agaaattttt tcttattcct ctcattatat atccttcctc ttcaggaggt cctatttcct
     1021 cacctctaac caatttaaga cctagttttg tcattattgg aactaccttt ctaagacttg
     1081 ctgctgaatt tcctgtagac actatatgca aatctaattt aaacatatct acacctggat
     1141 tttctttata catgcctgta attacaggaa tatttaactc ttcttcaact gccttacaaa
     1201 tagtcccaca agctactcca tatctacctg cattaaatgc tgggcctgca ataaataaat
     1261 cagggctttc ttcttttatc atttttaaaa gttcttgagt tgcactctca atattttcac
     1321 caaaatacgt atctccgcaa attacagttg agattatctc tgcatcatca ttaaattgac
     1381 tctgaaatgc tattccaggt cctactacac cagccctttt ttctggcgga atatctgctt
     1441 tttcctctcc acctatacca gcaaagaaat tgtttatata atgaaccact ctgattttac
     1501 tcatctctta atcctccaaa tcactttata gttgttacat caattagacc aatgattata
     1561 tcaacagaac tttatattca aatacttatt taataatatc ttgctgtcaa atgattatag
     1621 ccatttgcta tagttgatgc tattataatt tgtagctctg catccatgct tccgtcttca
     1681 tttaagcatc ctttataccc acctatcatc atttcaacat aatctaaagt tcctataatt
     1741 ttttccattg ctgggaaatg aaccactaga tttccttgac cacaagaaac aactgcatct
     1801 gcttccttag tagcgtctgc tatagattga ctcttgccat cttttccagg aaattcatcc
     1861 gttatcaaaa ctacatttgc tccaacttca ctacattttc tacaattcat cattaggtca
     1921 gtatctggat tcccataacc ttcttctgtc actagaactc catcaactcc taaaaattct
     1981 attaatttag ctaccatatc agagcttctc tctttatcga ctaagaatac gttctcatta
     2041 gtcaaaataa ctcctataaa gtttaaatct tttccatgtc ttttatataa gtcatgtata
     2101 actggattat ttaaatgatg gaatgttgta accttatcac atggtgctac acagtttcca
     2161 gatattatag ctccatccat tatttcagtt ggatacataa atgtaggcac tatctgttta
     2221 gcatccaccc catagtaata agtgtcatgt aaaagtcctt gtgattgtaa catgtgaaca
     2281 tatgctactg ttggcaggtc tggatattct tttacttgtt ttaataatgg ctttgtctca
     2341 tatacaatga cttcatctgg ctcaacttct cttcctgcct cacctacata tgcacctatt
     2401 tttaatcctg caattctagc tgcttcttca taatcatgag tatctatacc atcctttgct
     2461 tttataacaa tacatatatt atttgttttt gaaaaaggtg tatatttagc agtaggacca
     2521 ctcatatcaa ttacgccttc ttgaaatcca actatatttc cacaagttac aacacaagct
     2581 cctaaaagta catgggttct tccacttcct acagtcttca ctttatttat aattccagga
     2641 aatattttgc tttcatctcc aattttaact ctaggctcta ttacatcttt aactggagct
     2701 attcttattg attcgccagg tcttgcaagt tcaacattta cacttacaat cctttcatct
     2761 tctaatacta tcttttctat ttcttcgcta ctaatatata gtactccatc ttttacttct
     2821 gtcttttctc caaattcaac attttttata aatatattcc ctagctctag cttcatatga
     2881 aaatccccct taatatattt aatcgcaatt aaaagtattt attacgttta ttaatatata
     2941 aagcattttc tatgccaatt atttaaaata tatattctat gtattaatgt catagaaatt
     3001 ttcattttta atttacacca atattgaatt tagcaataat tattgtaaat attaattttt
     3061 aaaattataa taaaaaaaga taaacagatt ttttctgttt atcttttaat acagtttgag
     3121 aaattttttt ctcacttaat gagaagtttt tttctcactt aatattatac tctgatattt
     3181 tattggttaa ctgtcttcta ctaatctcca attttttagc tgtatttgaa atattatttc
     3241 cacacaaaga taaaactttt tcaatatact cacattcaaa ctcttttctt atttctctta
     3301 aaggtctaat acttatttta tcatctatgt atacattatt tgatattaaa tttaaattgt
     3361 cttttgataa attgccttct tcacttaaaa ctacaagtct atttattata ttttttaatt
     3421 ctcttatatt tcctggataa ttgtaattta ataaaaaatc ctttacttcc ttatcaatcg
     3481 agtgtatctt tttatcatgt tcaatttggt acttatttaa gaaaaattct ataagagtag
     3541 ttaagtcttc tcttctcttt cttagcggtg gtatagttat tgttattgta cttattctat
     3601 aaaaaaaatc ttctcttata tttccatttg atatttccac ttttggttct ttattcatag
     3661 cacaaattaa cctaaagtca actttaatag atttattact tcctatcctc tctatagatt
     3721 tgtcctcaag tgtcctcaaa agttttacct gaacatttag aggtatatct cctatttcat
     3781 ccaaaaatag agttccttta ttcgaaagtt caaatctacc ttttctacta tctactgctc
     3841 ctgtaaaaga acctttttca tgaccaaaca actcagactc caatagattt tctgaaaaag
     3901 aacaacaatt tacaggtaca aatatctcat tctttcttgg acttatgcta tgtatatatc
     3961 tagctaatat atcttttcct acaccagatt cccccaagat taatatatta acatctttac
     4021 aagcagcttt ttttgctatt tttatgatat tattaaaatc tctattttta gattccaaag
     4081 taaattccag attatttttt tgtacagaaa cagatatttt acttgccttt accttttcaa
     4141 cttcttctaa taaattttca atgggattac tttttataaa ataagataaa gctcctcttt
     4201 tcatagcatc aactgcattt tcaatacttc catatcctgt aactaatata acctctatat
     4261 ctctattaat agatttaatt ctatctaata gctgtactcc atccatattt ttcatcataa
     4321 catcagacaa aaccaaatca taatttttat cttttttaat tatatttata gcttcttcac
     4381 cacttagtgc tatatctaca agatagccct tcttttgtag aatttttttc attaccactc
     4441 catattctag ctcatcatca actaatagta ttttcattat atagtcacct ctttatttaa
     4501 tggaattttt atatgaaaac aagttccaac ccccaattta ctttcaacac ttatatctcc
     4561 attattcttt ttaatctcat tataagtaat gtataaacct aaaccagttc cctcaccaat
     4621 tggttttgtt gtatagaagg gattaaatat gtcctttagg gcatcttctt ttattccttc
     4681 accattatct ttaaaatcta taaataaaca ataatttttt tcataacagt atataataat
     4741 ttttccatct tgatgtattg catctattga attagaaatt aaatttataa atacatgttt
     4801 taacgattct ccatttatat agcatactaa attatgttca cagtcaattt ttatttcaac
     4861 attctttaac tgtaacattt tatattgaag tttcactata ttttcaataa aatttcttat
     4921 atttatatgt tctaaattat catctgatat tcttgcgaaa tttaacaagt tggttattat
     4981 attacttgcc ctatctacat tactttctat tgatttaaca caatcattta cttcttccat
     5041 tgtaacgtta tcttttaaga gatagcaatt atttcttatt atacccaaag gatttcttat
     5101 ctcatgtgct accccagaag ctaattgtcc tatagatatc attttgtttt ctctaagtaa
     5161 tttcgcctct actattttat cattagttat atctttaata agaactacta tatactcagt
     5221 attatttttt ttatactcta caggaaaagt gtttatcttt aaaattttat tgtgtatttc
     5281 tatttcacta gttttattag tattttcaga gaaggtattt tcaataacac cttttatcat
     5341 gctaatcaaa tctgctatct tataactcat ttcacccata aagtcatata gtactttgtt
     5401 cgattctact atattgttat tttcatctac aagcattatt atatctgtaa tactattaaa
     5461 agttgcctct aaagtttttt tattttctat tacctgttct gttttccttt tgatttctat
     5521 ctttaacaaa tatgtatagc tataaaaaac ataaattgat attaaaatta tacctataaa
     5581 aagataaata ctaggcacaa ttcctcgctc atacaatata tcatcaactt cattatacgt
     5641 agaataccat tttttcttta aatctttata cactccattt ttctgaagtt taaaaatgcc
     5701 tttatttaaa atactaacca gctcttcata ttgtttaggt acagcaagaa ctgctttttt
     5761 agtatatata ggattactta aaacactgta tttatttgaa agtccataat tatttatata
     5821 gtatcgcaat actggttcat caccaacaac tgcgtctact tttccagaca ttaaatggtt
     5881 gactccagtc tttatatctg gagtcaacaa aatgtttata tcttgaattt tttgctttaa
     5941 aaaatttata gaataatcac cttctggaat tgctactgtt ttccccttta aatccatgta
     6001 actatttata tcttgggact ttttatcttt caatatattt gcactcaaag tgtatattgg
     6061 gtcagtaaat ataaatttat ttgccctttc ttttgatgga atcaagtcaa aaaactttat
     6121 actatcatct attgaattta cgaaagattc tttccaccaa ttatttggtt taaaataaaa
     6181 atctctacca atttgaattg acagagaatt tattaaatca acaataattc ccttatactg
     6241 tccatcttcc ttacctttat atctcaaagg aggtgaactt tggtcagagc tgtaaatcaa
     6301 attcttgtgt ttttctaacc actttttttc ttcttctgta aatggaagtg acttcttaat
     6361 atattcaaat acatttagat tatactccat atttacatac atatttgtaa gtgaaaatac
     6421 tactaaaaat gaataaataa tccctataat aacaatcttt tttttattca ctttaaaact
     6481 ccaatcttct tttttactat aataaattca atatttaata acaaaatcct acttttttca
     6541 ttaaatattt atatttatta taaaactaat tgaatagatg ttgaaattat agtcattact
     6601 attcctatca atgcttcata tggaattaac ttaaatcttt cacctatatc catgtttata
     6661 ctacctgcac tagtatgaaa tagtgagcca tgtggtaatt gctcgaatac actagaccca
     6721 gcatttacta ttgaggcacc tgaaatcggt gatagcccac catttattat agcatcatga
     6781 aaagtagatg atgctatagt agcaccagct gttgatgatg ctgttgctaa tgacataagt
     6841 atacccgaaa ctggtgccaa taaaaattgt ggcatattta aaaattgtaa agcacctatt
     6901 gtactctgtt gtaattcaga catctgtata attcctgcga tagtccctgt tccaagtaat
     6961 aatatgcata ccccttgcat cttagataat ccaaaagcta aatattctct tgaattgttc
     7021 aagtttcctg tgacaattag agttacgatt cctcctattg gaagggctat cattgggtct
     7081 ataactatag gagatacatt tcctaaaaac aatagaaata tagctataat tggaccacat
     7141 aatgatttaa ataaacttgg taaatcttct ctttgctcta aaatttcata tgattgtact
     7201 ttatttcctt tatttattaa tatacttgct aaaataactg taataacaac tccaataatt
     7261 gctggaatta tatttgccat cataacacta gagagattga cagaaaaatt atctgctact
     7321 gctatagtat ttggatttgg tgatattata ttacctgctt taccccctcc caacatagct
     7381 aatagtattg acaatttaga ataatttaat tttctaccta tatataatcc tataggtgct
     7441 acagttatta tagatacatc caaatttacg cctactccag caagtaccat agttgaaatg
     7501 gcaatcgaaa ataaagccct cttttcacca aatattttta caatctgttc tgatatttta
     7561 tctactgccc ctgtttttat taaacttcca gctaatacac ctgatgccaa agctctaaga
     7621 attgatggtg atatattcat agctccattt gccataaaat ctacagtttg tgttatattt
     7681 gctcctccaa ctaatcctcc aataaatgct cctagcatta aactatatac agcttgaaat
     7741 ttttttataa ttaaaataat tgctattaat aaaccaaata tagctccaaa agtcgttatt
     7801 tgcataatat cccctcctat cataaattaa ctatcttttg cttaatttta tttaaatata
     7861 ttttaagtat tctgtatcca ctttgtcaat tatattttaa tttatttaat tatattgaac
     7921 atagtttttt caaaacctaa ttctatatgt tataataatt tcatactaca taatcatttt
     7981 acttgattat tttaagttaa tattttttaa attaaaaaga gaggatattt atatgaatga
     8041 tttaaattta ggggaaaaaa tagctgaagt tcgaaaaaaa caaaacttaa gtattcgaga
     8101 tttagcaaaa ctagctgatg ttacaccatc attattgagc caaatagaac gaggtcttgc
     8161 taatccatct gtaaattcat taaaatctat agcctcttct ctaaacgttc ctttatttac
     8221 attttttgta tctgaggtag ataaaaaaaa cttgatagta agacatgata atagaaaaaa
     8281 agttatttta cctggaagca aagaagttat atatgagatt ttaattccag actctagtgg
     8341 taatttagaa tttgcaatta tggatttagc tccaaacact tcttcttgtg ttgataggat
     8401 tactcataat ggagatgaaa ttgcatatgt tttggaggga gaagttaaac tttttatgga
     8461 tgatgatgaa tttactttgt ccaaaggaga tagtgtaaaa gttcctttag gaacaaaaca
     8521 taaatggcaa aatgactcaa atagtgaaag taaagtaatt tttgcagtca tcttgtaaag
     8581 gagtaaaaac catgaagaca tataaattta tattttttga tgcagatgat actttatttg
     8641 attttaagaa aagtgaatca catgctttta aaaaacttct atctgagttt gatttagaat
     8701 ttaattttga gaattatata gagtcatata gaaatataag tgataagctt tggctcgacc
     8761 ttgaaaaaaa tattataact ttgaatgaac ttaaattact tagatttgaa ctttttgcaa
     8821 ataaaatatc tttggatgtt gattctgaaa ctcttagcaa aatgtactta aattttttag
     8881 gtgaatgtac atttttaatt ccaggtgcaa tcgatatatt acaatatctt aaaaaaaagt
     8941 atactattgt tataataaca aatggaattt ctgcagttca aaagaaaaga cttgaaaatt
     9001 caaaaataaa agaatatatt gatggaatgg ttgtatctga agagttgaaa atatcaaaac
     9061 caaatcctga aatatttaaa tacgctctca aaaagtttaa ttgtcatgat aaatcttctg
     9121 ccttaataat tggggatagc ttaacctctg atatcttagg tggaataaat tcaggaatag
     9181 atacttgttg gttaaattcc aataacagta taaattatac taatcatata ccaacttatg
     9241 aaataaatac acttattgaa ttaaagaaac tactatagtt tgtaagtctg ttcaaattaa
     9301 atagaatatt actttgcaat aattttttta gtttatacat aaaatgacca aaaaaattat
     9361 tgcaatttat acacaaaaca aatcttagtc ttgaaaatta actaaaagca ttttctagga
     9421 cacttactac atcttgattc taaattttct tcatccccca ttaaaataag cattgcaaca
     9481 ctttttatgg gggatagttg atgataatca ttaatttcta tttctatact attatattta
     9541 ttttttacat agttataaat atccttttgt ctctctactt caaaatcttt atctcctgga
     9601 tatatttcat gcgatatttt gagactatca tactttcctt ttaaatacat ttttattttc
     9661 tcattaatac aatctaaaca tactatacct accttatcca atgccagccc tgccatcata
     9721 ttgcaatttt ccatatagtc attcatctta aaatcaattt tatcccctat tgtatatagt
     9781 acagcaaatg cttttttaca ttctttaggt gttttattaa tatcaacttc acttatatat
     9841 atcttgatat ctaaaatatc atttatattt cttatttctc tttcaacgat tttactaatt
     9901 gtatctggag cttttctatt tccatacccc aaaaacttta gcacaatatt cttatctata
     9961 aaaatatcat ctaaatttat atcctttaaa agatatttat ttttattact attataattc
    10021 atcaatatca atcctatcta attctttata actaattctt cctgccttta tagctgcttg
    10081 taaattttct actggagtgg tatttaagca gttacagcca aaatcacata aaaagttatc
    10141 atatcttctc atcttcttta acaattttag tgtctctctt ctaatcttat ctggcttaga
    10201 acgtccaaga agttttattg ggtctaaatt accaatcaaa actatatctc taggaatcat
    10261 tggagcaatt tcttcatagt ctaaaatttg gtctaaactt actgcatcta tattacaact
    10321 tagcatatta tctaataatt ctctggtgtc tccacatata tgcatacctt tccaacaatc
    10381 aagttcattg tagattttat ttaaattttt tactataaat tcatcaaatc tatctttact
    10441 tagggttact gatgttggct ctgctattga aatatatttt gctcctgctt tatttgcagc
    10501 tacagcatat cttcttacca attcagttgt aaattccaat aattcttcta caaatttttt
    10561 atcagttatt atacatctta aaagttgagt agctccagct aattgacctg caagtgtaaa
    10621 tggaccttgt atcgattcat atagtggttt tttaatactt tttgatataa ctgataagct
    10681 ctctatattg agtggcatac gtccactcgt ataagggtct ggaacatcta attttcttaa
    10741 tttatctctg tcagttatag tatgtgtcaa tacacttgga aaatcataat ctggtcttaa
    10801 tgtatctaaa cccagagttt cacaaaatgt aatcccatca cagaatgagt atataaaatc
    10861 tgattcaaaa tacttgtcca tagtctttgc taattctaat tgcaaatcta cactatcata
    10921 tacttcatat gcctttttat ttattaagaa caatccattt gtacccatat caggcagaaa
    10981 aaaacctcta tcttcactat ttatatactg aagtaaagtc atatctgaaa acatttaaac
    11041 ttcacctcct ataagtttat ttattaattt aactgctttt ggagcatttg aactgtatcc
    11101 atctgctcct atttcttctg caaactccat acttacaggt cctccaccaa ttattacttt
    11161 tggtctttta tctatctcaa tcatattaag ttcgtctata accgatttca tattttccat
    11221 tgtagttgtc atcattgaag ataagcctat tatatctaca ttgtttttta tagcttcttc
    11281 tattatatct tcactttttc tatttacacc taagtcaata accttatatc cagtggcttc
    11341 taccattact tttacaatat tttttcctat ctcatgagta tctccttcta ctacagacat
    11401 cagaattact ccctttgatt ttttctttac ttttccagtt cccttcaaaa gattaatacc
    11461 tttatttaat gcatctgtgc atactataac ttctgaaaga tagtattcct tattctcaaa
    11521 cttatctaaa gctcctatca tacctttgtt taaaccatat tcataaatat cttccacatc
    11581 aatattttta tctatagcaa tttttactaa attctctata ttatctacac ccatatttaa
    11641 tatacattct gctatatcat caagtatgtc catataatac tcatcctttc taattactta
    11701 caaaattgct ttttcaatat ccaataattt ctcttattta acaaggacta actctaggta
    11761 tcaatcaatt ttatttaata tcatctaaaa ctattgataa tttaaatttg cagtccatgg
    11821 aaaatctaca ggaaatgcct ttgtagcaaa ttca
//
LOCUS       DAEMGG010000119        11848 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEMGG010000119 DAEMGG010000000
VERSION     DAEMGG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11848
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.120"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..617
                     /locus_tag="KPK24_003028"
     CDS             <1..617
                     /locus_tag="KPK24_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300680.1"
                     /translation="PFGITEYINGKYKFKNKIWEDFFETKNQNIYKALLEISNYFKSK
                     GLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAPAGLPFEFERFIDESIH
                     KNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNKDIYVGVEINKKEIISP
                     VTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWFRN"
     gene            630..2126
                     /locus_tag="KPK24_003029"
     CDS             630..2126
                     /locus_tag="KPK24_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG2300681.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEECMKICEDFDFNIDPHMVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVND
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            2149..3099
                     /locus_tag="KPK24_003030"
     CDS             2149..3099
                     /locus_tag="KPK24_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300682.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            3120..4082
                     /locus_tag="KPK24_003031"
     CDS             3120..4082
                     /locus_tag="KPK24_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG2300683.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            4454..5086
                     /locus_tag="KPK24_003032"
     CDS             4454..5086
                     /locus_tag="KPK24_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2300684.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            5497..5589
                     /gene="kdpF"
                     /locus_tag="KPK24_003033"
     CDS             5497..5589
                     /gene="kdpF"
                     /locus_tag="KPK24_003033"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG2300685.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            5630..7312
                     /gene="kdpA"
                     /locus_tag="KPK24_003034"
     CDS             5630..7312
                     /gene="kdpA"
                     /locus_tag="KPK24_003034"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG2300686.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            7337..9394
                     /gene="kdpB"
                     /locus_tag="KPK24_003035"
     CDS             7337..9394
                     /gene="kdpB"
                     /locus_tag="KPK24_003035"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG2300687.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            9413..10039
                     /locus_tag="KPK24_003036"
     CDS             9413..10039
                     /locus_tag="KPK24_003036"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG2300688.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVEDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            10276..11184
                     /gene="cysK"
                     /locus_tag="KPK24_003037"
     CDS             10276..11184
                     /gene="cysK"
                     /locus_tag="KPK24_003037"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG2300689.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            11211..11801
                     /gene="cysE"
                     /locus_tag="KPK24_003038"
     CDS             11211..11801
                     /gene="cysE"
                     /locus_tag="KPK24_003038"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG2300690.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
BASE COUNT         4511 a         1270 c         2318 g         3749 t
ORIGIN      
        1 ctccatttgg tataacagaa tacattaatg gaaaatacaa atttaaaaat aaaatatggg
       61 aagatttttt tgaaacaaaa aatcaaaata tatacaaggc attattagag ataagtaatt
      121 attttaaaag taagggattg aaaataggta tagatacttt ttctcctttt atttcttact
      181 ttacaggtca agatttagaa aaattacaag aagtagcaga ttttataaaa ccaatgatgt
      241 atagaactac aaaagctcca gctggtttac catttgaatt tgaaaggttt attgatgaat
      301 ccatacataa gaataaagag attggaaaaa gaaatttctt agatatttta ggaattaagg
      361 agtatgaagg agatgtatat cctatagaat ttgtactaga ggaaatcgat tttatgacaa
      421 gaaatcttaa taaagatata tatgtaggag ttgaaataaa taaaaaggaa attatatctc
      481 ctgttacacc taagtacata agtgaaaata tttcaaatat aaataagaca acagtaaaag
      541 gatatgtttt atcttgggat ttgttgtcag caccaaaaga aaatattgat gaaattataa
      601 aatggtttcg gaattagtga ggtgataaag tgagtaagat attggaaatg caacaaatat
      661 ataaaagttt tggaaaggta gatgttttaa aagggataaa ttttgagtta gaaaagggag
      721 aagtaagagc tcttttggga gctaatggag caggaaaatc tacacttatt aaaattttag
      781 gaggagttca ttctcaaact aaaggtaaag taattttgac tggtaatgaa ataatgttta
      841 aaaatccaca tcattctaaa gaaaatggta ttagtataat ttatcaagaa ttaagcttga
      901 taccaacgct tacagtactt gaaaatattt ttcttggtag agaaatttta aaaggaagtg
      961 tgtttttaaa caagaaagac atgcttgagg aatgtatgaa aatttgtgag gattttgatt
     1021 ttaatataga cccacatatg gtagtttcta atttaagtgt agcaaaccaa caaatggttg
     1081 agataatgaa agctgtttca tgtaattctg aaattatcat aatggatgaa cctacaacat
     1141 cacttactaa taatgaaaaa gatgggctat ttaaaataat aagtagacta aagtctaaag
     1201 gtaaaacaat agtctatatt tctcacatat tagaggaagt ttttaaagta tgtgatagtg
     1261 caagtataat gaaaaatgga attatgattg gaacttatga tgtagaggat ttaactaaat
     1321 taaagataac tcaacttatg acaggaaatg ataatcacaa acttattgag aagaaggatt
     1381 atacatttgc aaaatatgaa gagccaccag tactagaagt aaagaatata agctggtcta
     1441 acaaaataaa aaatgtttca tttaaagttt atagaggcga agttgtaggt cttgcaggtc
     1501 ttgtaggttc aagaagaact gaaattataa atttgatatt tggtatagat aataaagata
     1561 gtggagacat ttttatcaat ggtaaaaaag tagacataac ttctcctaag gttgctataa
     1621 aaaacaaaat aggattgatt cctgaagata gaaaaaatct aggattaatt ttagaacaag
     1681 ctatatacac aaactcatca tcagtacaaa ttgataaatt caagaaattc ttgtttatag
     1741 atagaaataa agaaattgag ttttctaaaa aaggtgttaa ggaattagga ataaaagtta
     1801 atgatgtaaa gcaagaagta aaagagctta gtggtggaaa tcaacaaaaa gtagtagttt
     1861 ctaagtggtt agagaaagat ttagatttaa taatatatga tgagccgaca aagggaatag
     1921 atattgctgc aaaagaagat attttcaata caatcaaaga attttcaagt aaaggaatag
     1981 gagtaatatt tatatcttct gatttagaag aagtaataag agtaagtgat agaattttag
     2041 taactagaga aggttctatt gtagatgatt taaaaaatga gaatatacag gttcaagata
     2101 taatgaacag tatatttaat gtataaaaaa tgaaaatgga ggactgaaat gaagaaagct
     2161 tttgagaagt taaatttaag agattatgga gtattattag gttttttagc tttatgtata
     2221 gctataagta ttgcaactcc atcattttta ggtaaacaaa atatacttaa tcttttacga
     2281 caatcttcta taattggaat aatttcagca ggtatgactt ttgttataat atctggaaac
     2341 tttgatatat cagttggagc agtagcagct cttgcaggtg ctataacaat gaaatttgcg
     2401 actggtggaa ctaatttgtt tgcatccatg ctcttaggtg tagcgatttg tgcagtaata
     2461 ggattaatca atggtatagt agttgcaaag ataaatgtac catcattaat agccactatg
     2521 gctatggtca ctattgtaag aggattgctg ttgatgctta caggaggata tccaattact
     2581 gaaaatatac caatacttga ttacattgga aatggatatt tgtttaatat accaatacca
     2641 gtagtaattt tctttatagt ggtgttagct tcatttattg tacttaataa aactaagttt
     2701 ggtagatatg tttattctgt aggagggaat caagaagcaa gtaaacttaa tggtataaat
     2761 gtagattctc acaaagtgaa agtatttatt attaatgctg tacttgcagg aatagctgga
     2821 atcgtactaa caggaagact tgggactgca actgcgattg caggtgaagg atatgatatg
     2881 gatgcaattg catctgtagt aataggaggt acatcagtgg caggaggttc tggttctgta
     2941 cttaaaactg ttataggagt attacttatg agtgttataa acaacagttt taatcttctt
     3001 ggaatagatg tatatttcca gtacatattt aaaggattaa taattttagc agcagttgga
     3061 tttgattctt atagcaagaa aaaacttgct tcaagataaa aaataagggg gattttttta
     3121 tgaaaaaatt aatgaaacat ttagcattgt tgctatcact tgttatgata tttggtttag
     3181 ttggttgttc aaatggtggt gacaaagaaa aaaatggaga aaagaaaata gcagtattac
     3241 taccaggttc aacaggttac tttgtagcaa ctaaacaagg tatagatgca aaatctaaag
     3301 aattaggtgt aagcgtggaa tatgcagatg ctcaatggga tgctagtaaa caattatcac
     3361 aagctgaaga ttttatggct aaaggggtag acatgattgc attatgtgga gttgattctg
     3421 ctgtaagtga gagaatagtt aaagctgcta atgattcaga cgttcctata gtagcattta
     3481 caaattctat aggaagtaac ccaactggag aatttaaagg tttagttaca tatataggac
     3541 aaaatgagga agaaacagga gcattaactg gaaaaattgc taaaaactta ttaggagaaa
     3601 ctggtgggaa agctgtatta atagaaggag taccaggaac tactccacaa gtattaagaa
     3661 aaaaaggtct tgaaaaagaa ttaaaagata gtaatataga aatagtttat aatcaaactt
     3721 caaattggga gaaagagcaa gctttaaagg taacagaaga tttaattcaa aagaaaactg
     3781 attttaatgt tataatatgt caagatgata attctgctac aggtgcaggt caagctttaa
     3841 aagatgctgg tcttaaggat aaagttaaag tcataggttt gggtggaagt aaagatggat
     3901 taaaagctat aactgatggg ttaatagatg gtacaactta tatgtctgct gtagaagaag
     3961 gtggattagt catagaaaag gcatctaaat tccttaaagg tgaaaaaata gaacctgtta
     4021 cacaaattaa acaagttgaa gttaacaaag ataatatttc agagttcaag ggtgaatggt
     4081 aaattgtcat agataataaa taagattttt agagttactt tagaaactat ataaagtaac
     4141 tcttttttta atttaattta gtgataaatt attgaatagt atttctaaac aaataaaaaa
     4201 ttaatataaa caaagactta attatactaa attataagta aaactaatat aaacttatgg
     4261 aaaaaaggaa aacgtctaaa gtattggaat tactatagtg taggtgtggt attaaaaaaa
     4321 cataaaaaaa cataaaaaaa ttgttgaaaa aaatatagaa attttgtata attaaagatt
     4381 tgaaaagtgc ggtatacaaa gccaattctt ttgtatataa tatatataaa ttcagaaaaa
     4441 ggaagggata tgtatggcca ggcggttatt tttagaaagg attggactta tatttttagg
     4501 ttcagcaata ttagcatttg gagtttataa tttttattat ttaaacaaca taacagaagg
     4561 tggagtgtta ggtattttac tactgttaaa aaatttattc aatatacaac cagcaatagc
     4621 aaatgttgta atagatggtt tgctactttt agtaggatat aaatttttcg gtaaaaaatt
     4681 ccttatatat tcaatagtag cttctataac tttttcagtg ttatatgact tatttgaagc
     4741 tataggacct ctagtaccac aatcacaaaa tatgctttta tcaactattt tggcaggtgt
     4801 aactgttgga actggtgtag gtatagtagt aaaagcaggt tgtgcatcag gaggagacga
     4861 tgctttagca ttagtaattt ctaagactac atctttgaac atagggcaag tttatttagc
     4921 aacagatgtt attgtattgt tgttatcatt attttatcta tcagcgtttg atatctttta
     4981 ttctttgatt gctgttacaa ttagtggcaa ggtaattgat tttatttatt atcatggtaa
     5041 aagtttggat atggatataa gtaatgatat agttccagaa tgttaatata aattaaataa
     5101 ctttagattt tattaggggt ggcagatttg ccaccttttt tgtgtctaaa tctttataga
     5161 aagtataatc ctatctaggt ttttgcaaat ctcaaatata gaattatgtt gaaatgactg
     5221 ggaatacgat tgctagatgt gcattttaaa aacccaattt tatatttatg aaatactaat
     5281 cattaatgga tgtttcttaa tcctatcttt atacctaaaa tgataagata aatgaagtag
     5341 caatacaaaa ctaaaatttg taaaagagat taaattaaat ggaggagcta aaatgttgga
     5401 tatagttatg atagcattgt tattcatagg atttgtttcc atgaagttac ttgctgattg
     5461 gtgtgggaaa caaactgaaa aaaaataatg taatgtatgg agggattacc tatgtggttt
     5521 ctggcagttg taattataat gttaataata tatttggtat atgcgcttat taatcctgaa
     5581 aaattttaaa cagtataagg ctaataagta agttgaattg gggggaacaa tttttatggt
     5641 gcagatagta atagtgttag cgatttttat gattttagta atacctatgg gcaagtattt
     5701 atatcatata gctactaatc aaaaaacatt tggagataga ctatttgata aagtggacaa
     5761 ttttatatat aaggtatgta gtatagataa gaaaaaagaa atgaactgga aacagtatgc
     5821 attagcttta ttatttacaa atgcagttat ggtatttata ggatatatca tacttagaac
     5881 tcaaagtatg catattttta atccaagtgg aattaaaagt atggaacaag gactatcatt
     5941 taatacaata ataagtttta tgacaaatac aaatcttcaa cattattcag gtgaatcagg
     6001 actttcatac tttagtcaga tgacagtgat aatatatatg atgtttacat cagctgcaac
     6061 aggatatgcg gctgctatgg catttgtaag aggtctagtc ggtaaaaaga aaacattggg
     6121 aaatttttat gtagatttaa ttaggataac aacaagagta cttttaccag gagctttaat
     6181 tattgggctt atattagtta cacaaggagt accacaaact tttgctggga cagaaacagt
     6241 gactactata gaaggaaagc ttcaagatat agctagaggt cctgtggcag ctcttgaatc
     6301 aataaaacat ttaggaacaa atggtggtgg attctttggt tcaaattcat cacacccatt
     6361 tgaaaatcca acaataatat caaatatagt agaaatatta tctatgatga tattaccagg
     6421 agcttgtgtt gttgcatttg gtcacatgat aaagaataaa aaacaaggtt gggttgtgtt
     6481 tggcgcaatg tcaatcatat ttttaatagg tctagtagtg tgctttaaag cagaaagtgc
     6541 aggaaatcct attttatcac aattagggct taatcaaagt atgggaagta tggaaggtaa
     6601 ggaagttaga tttggtatag cacaatcatc attattcaca acagtgacta cttcctttac
     6661 gacaggtaca gttaataata tgcatgatac actgactcct ttaggaggat tagtaccact
     6721 tttaaatatg atgctaaatg tagtatttgg tggtaaaggc gtaggtctga tgaatatgtt
     6781 gatgtacgct ataatagcag tattcttatg tggattaatg gtaggaagaa caccagagtt
     6841 tttaactaag aaaatcgaag gaaaagaaat gaagcttatt gctttactta ttatattaca
     6901 tccactttta atactaatgt tctctggtct ttcagtagca ataccagcag gacttgaagg
     6961 aatttcaaat cctggattcc atggattatc acaggtattg tatgagtttg catcttcagc
     7021 agccaataat ggttcaggat ttgaaggtct aggagataac actatgtttt ggaatataac
     7081 aacaggtata gttatgttct ttggaagata tgtgtctatt atagtactac tagcaatatc
     7141 aagtttgtta gcatctaaaa aagctgtaaa tgaaagtata ggaacactta gaacggataa
     7201 ttttacattt acaatagttt tagtacttgt tgttttaatt gttggtgctt taacattttt
     7261 cccagcctta gcgcttggac ctatttcgga gcatctagtg ttgtggcact aaaaaattgg
     7321 gagggagtaa agttttatgg aaactaaaaa aacaaagttt attacaaaag atattttcaa
     7381 aacttccatt atagaagctt ttaaaaaatt aaatcctaaa tatatgatga aaaatccagt
     7441 tatgtttgtt gtagaaattg gatttatagt aacattgata cttacaatag tacctagttt
     7501 gtttggtgat gaaggcaata acttaagagc ttataatggg attgtaacaa taatcttatt
     7561 tataacagtc ctttttgcaa attttgctga agctgtagct gaaggaagag gtaaagctca
     7621 agcagataca cttaagaaaa ctagaaaaga tacagtagca agggttatag atacgttagg
     7681 aaatgaaaaa atgataagtg caagtgagct aaaacttggt gatattgttt tagtaaatgc
     7741 tggagaagtt atcccaaatg atggagaagt tatagagggt atagcttctg tggatgagtc
     7801 agcaataact ggagaatctg cacctgtcat gagagaatca ggtggagatt tcgcatctgt
     7861 tacaggtggt actacagttg taagtgactg gttaaagata agaataacaa caaaaccagg
     7921 agaatcgttc ttagataaaa tgatagctct tgtagaaggt gcttcaagac aaaaaacgcc
     7981 aaatgagatt gctttaaata ctttgttagt agggttaaca cttatattta tggttgtttt
     8041 aatatctctt tatccaatgg caaactatgc aggtgtaaag attccaattt ctactatgat
     8101 agctctcttg gtatgtttaa taccaactac tataggtgga ttgttgtcag ctattggtat
     8161 tgcaggtatg gatagagtaa ctagatttaa tgtaattgca atgtcaggaa aagcagtaga
     8221 agcttgtgga gacgttgata ctatgatttt ggataagaca ggtacgatta cttatgggaa
     8281 tagacttgca tcagaattta tagcagttgg aaatgcagat tcaaaagatt taataaatta
     8341 ttctgttatg tgttcattaa aagatgatac accagaagga aaatctatag ttgatttagg
     8401 tatgaaatta ggttcaacag gtaagacaaa agaggcagag gaagctgagt ttgtagaatt
     8461 ttctgcacaa actagaatga gtggtattaa cttagctgat ggtagagcta taagaaaagg
     8521 tgcttatgat agcataataa aaagagtaaa agaatctggt ggtacagtgc cagaagattt
     8581 agaagaaaat gtaaatcgag tagctaaact aggagggaca cctttagtag tgtgtgtaga
     8641 taatgagata tatggaatta tctacttaaa agatacagta aaatcagggc ttgtagaaag
     8701 atttgctaga cttcgagaga taggaataaa gacaataatg tgtacagggg ataatccctt
     8761 gacagcagct acaattgcaa gagaagctgg agtagatgga tttattgcag aatgtaaacc
     8821 agaagataaa atagatgcaa taaaaaaaga acaattagag ggtaaaatag ttgcaatgac
     8881 aggtgatgga acaaatgatg ctcctgcact tgctcaggca gatgtaggtc ttgcaatgaa
     8941 tagtggtaca acatctgcaa aagaagcagc aaatatggtg gatttagatt cagacccaac
     9001 taagatattg gaagttgttg aaataggaaa acagctttta ataactcgtg gggctttgac
     9061 aacatttagt atagcaaatg atgttgcaaa atattttgcg attataccag ctatatttac
     9121 tcttgcaata cctcaaatgg agatattaaa tgtaatgaaa ctttctacgt catttagtgc
     9181 gatattatca gctttaatat ttaatgctat aattatccca tgtttaatac ctcttgcaat
     9241 gaagggagta aaatatagac ctatgaaatc acaagctatg ttaatgagaa atatgcttat
     9301 atttggtctt ggtggagtta tagtaccatt tttaggaata aaattaattg atttattgat
     9361 tacaccttta gtaatggttc ttaatttagg ttaataaatg gaggatataa aaatgaaaac
     9421 attaaagcca gcattacttg taagtattgt attgttagtt gtttgtggac ttgtatatcc
     9481 acttgttttg acaggtgtaa gccaagttgc atttaaagat aaagctaatg gaagtatgat
     9541 agaagtaaat ggtgtaaaag taggttcaga acttattggt caaagcttta cagatgcgag
     9601 attctttaag ggaagagtat catcagtaaa ctataatact tatacaaaag aagatttagt
     9661 accagataaa gatggaaaca catcttatgg aggtgtatca tcagggtctt ttaattatgg
     9721 agctacaaat ccagaacttc atgatagagt acaaaaagat atagaacagt ttttaaagga
     9781 taatccaact gtcaaaagag aggatatacc aacagattta cttacagcat caggttcagg
     9841 acttgaccct aatataagtc cagcctctgc aaaaattcag ataccagcta tagcaaaagc
     9901 gtctggaata tcagaaagta aattacaaaa aattgtagaa gataatactt ccaaaaaact
     9961 ttttggagtt ttaggtgaag atagagttaa tgtattaaag gttaatgtag aggtcgcaaa
    10021 aatattaggt cttatatagt attttgtgga attttagcaa caaaatatta ctcaagattt
    10081 aatttaaagg ttaaatttta aagagtgaaa atcgataatg attttcactt ttttatttga
    10141 atattactaa tatttggaat aaagtgattg taagtataaa atccaaaaaa aggttgcatt
    10201 atcaaaatga taatactata atatatttat cgttatcaat ttgataatag atttggacat
    10261 ataggaggaa atagtatgtt atataataac gcattagagt tgataggaaa cacacctgta
    10321 gtaaggctga ataatttagg ctgtactaat gggtgttcta acatatatgt aaaattagaa
    10381 aaagtaaatc cagcaggaag tataaaagat agagctgttt atgaaatgtt agagggttta
    10441 gagcaaagag gagaattaaa aaagggagat gtactagttg aagcaactag tggaaatact
    10501 ggtattgcat tatctatgat aggaaaatta aaaggatatg aagtagtaat cgtaatgccc
    10561 gaaacaatga gcgttgagag aagagattta atgaaggctt atggagcaaa tttggtgctt
    10621 acagatggta aagctggaat ggcaggttcc atagaaaaag caaatgaact tttaaaagaa
    10681 aatccaaact ataaaagctt aaaacaattt gaaaataaag ataatccaaa tgcacattat
    10741 aaaacaacag cagttgaaat tatgaatgat gtcaaagatt tagatatatt cgtatgtgga
    10801 gttggaacag gtggtacttt aataggaact gcaagatatt taaaagaaca aaatccaaat
    10861 ataagagtaa ttgcacttga accaaaaaaa tctccagcta tatcagaaaa caagactgga
    10921 cctcataaaa tacaaggaat aggtgctaac tttgttcctg aaaattatga ttctagtgta
    10981 gttgatgaag taatattagt agatgatgaa gatgcatttg atacagttaa attacttgca
    11041 gcaaaagagg gaattttagt agggatatct tctggagcaa atatatttgg tgctatagag
    11101 atggcaaaaa agtatccaga taaaaagata gttacagtag ctccagatgg agttgataag
    11161 tatatgtcta tgggaatatt ttaataattt tatttgaggg agtgaatact ttgtttaaga
    11221 aaataaataa agatattgaa tatataatga agaatgatcc agcagctagg tctaagatag
    11281 aagttttttt actttatcca tcagttcatg caatgataat gcataggatg gctcatgctc
    11341 tttataagaa gaagaagctt tttacagcaa gattaatatc ccagatatcc agatttatga
    11401 ctggcataga aatacatcct ggtgctaaaa tgggggaagg tatattgata gaccatggaa
    11461 tgggtgttgt aattggagag acagctgaag taggtaatag ggtgacaata tatcaaggag
    11521 ctactttagg agctacagga aaagatactg gaaagagaca tccaactgtt ggtgatgatg
    11581 tattaattgg tgctggaaca aaaatactag gacctttaaa tataggttca aattcaaaaa
    11641 ttggtgcaaa ttctgttgtc gttaaggatg tacctaatgg tgcaacagtt gttggtatac
    11701 cagcaaaaat agtaaaaata agaaacctag aacctgtaaa aaagaataaa aaagaagtgt
    11761 cctatgagta tgatgaatta gataatgtat attatatata ggatttatat aactataaaa
    11821 taaactttat aaaaattatt ttaaaagg
//
LOCUS       DAEMGG010000120        11732 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEMGG010000120 DAEMGG010000000
VERSION     DAEMGG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.121"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..688
                     /locus_tag="KPK24_003039"
     CDS             <1..688
                     /locus_tag="KPK24_003039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300691.1"
                     /translation="SQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNIMKQK
                     QELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEEKNKS
                     TMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKKEKVS
                     EDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIFDKTD
                     EQKKHINVLI"
     gene            866..1756
                     /locus_tag="KPK24_003040"
     CDS             866..1756
                     /locus_tag="KPK24_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300692.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2149..3105)
                     /locus_tag="KPK24_003041"
     CDS             complement(2149..3105)
                     /locus_tag="KPK24_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2300693.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3808..4803
                     /locus_tag="KPK24_003042"
     CDS             3808..4803
                     /locus_tag="KPK24_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2300694.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4781..5644
                     /locus_tag="KPK24_003043"
     CDS             4781..5644
                     /locus_tag="KPK24_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG2300695.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFKMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            5641..6435
                     /locus_tag="KPK24_003044"
     CDS             5641..6435
                     /locus_tag="KPK24_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG2300696.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQERELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGIFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6720..8099
                     /locus_tag="KPK24_003045"
     CDS             6720..8099
                     /locus_tag="KPK24_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2300697.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIF
                     KYKNRYKYM"
     gene            8547..9260
                     /locus_tag="KPK24_003046"
     CDS             8547..9260
                     /locus_tag="KPK24_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300698.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(9532..11070)
                     /locus_tag="KPK24_003047"
     CDS             complement(9532..11070)
                     /locus_tag="KPK24_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2300699.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVREL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         4278 a         1342 c         1783 g         4329 t
ORIGIN      
        1 tagtcaatat agaagtcaat attcatttag atatgaaagt aatataaata atacaagaat
       61 aaatgaaagt atggttaaga aaaatgaaac tgtaggaaaa gacacttatt tatctaatat
      121 tatgaaacaa aagcaagaac ttaatgatag aattagagat ttaaaatata gacaagaggt
      181 ttatactaaa aaaataaatg acgcaattaa gaacttatgt aaatcagaaa taagagaaac
      241 aactaataat ttttctaata tagaaatagg tattaaaaat agcattatag aagagaaaaa
      301 taaaagtaca atgttagatg aaaattcaac ttatctaaat acaaatgatg aaaaagaatc
      361 tttaatcact aaagagtcta atgaaaaaat tgaagaagaa atattaaatg atgaaaaatt
      421 agaagagtta gaacaaaaaa aggattataa agaggattct aataaaaaag agaaagtatc
      481 tgaggactta tctttagtag gtaaaactcg tgaagagctt gaaaatatgc ttaaaaattt
      541 tataaattta acacaagaag aaataatgaa acttgagtcg agaatagaaa agttagataa
      601 aaatgctgaa gaatacaaac aaaattcaaa gactaatata tttgataaaa cagatgaaca
      661 aaaaaaacat ataaatgtac tgatttaaac aatatgtttt aaaaaaataa gtttattata
      721 taatgaaaat ataaaggtac aatttcaata cacttctatt aaaccaataa tttatgaatc
      781 agatttaatt tcaatttact aataaccata tatttatgag ttaataattt tatgttttaa
      841 aaagaattaa ataaggggga gtagtatgaa gaagaatata ttaactacta tgattaccat
      901 gattattata ttgatgatga gtatgacagt aatggcagag ccatatcaag aagatgaaac
      961 atctagcttg aaaaaacaga gtgcaaatga acgtaatcag actaatatag gcacaatgga
     1021 tggcagagtg caaacaaatg caataagtaa aggtgctaat tatgacccta aatatcctct
     1081 taaaggaatg ttaacacaac ttggtcttaa tacaacaggt aaagagggat gggatttact
     1141 ctatggagga aatcccaatg gagttgagct tcgagcagat ggtaaatatt attatataaa
     1201 taagggtatg gaagggctag gattttatgc ggcagcattg tcaggtggct catatcagag
     1261 aataggcttt ggaaaacttg accctataga agtaatagct gatggaaatt ggatttctta
     1321 caaacaagct caagatacac ttacagtaat caggaatttt ttaaatagct ttgactggag
     1381 aaatgcatct gagatggaaa gagctaatag agctgcaaaa ttggtaaccg aagcaaaata
     1441 tgcagactct aaatattgta acatagtcta tggtaatctg gttgataaac gtggtgtatg
     1501 tggaagtttt gcaagtagct ttcatttatt aacaaggtta atgggaatgg attcactatc
     1561 tatattaaat ccatcattaa atcatgcttg gaactatata caaattgatg ggaaatggta
     1621 taggtttgat gggtctgaaa tatcagcttt tggtggatca ttagactttg attaccgtaa
     1681 attaaaggat gcaacaagag agatgactac ttactatgat gctaaggcat tgtctatatt
     1741 aggatttaat caataaaaat agggggaggc ttcttataga gccttttttt attgatttga
     1801 aatttaataa ggaattttat atgaaaattt aatcaataaa agcaaaaaag gctctttata
     1861 aagcctttct aagtgttttt ttaagtaaaa ttttttatat caacatcagt tttaatcaaa
     1921 acatataaca ttttggactt acttttaaaa ataaaattat taaaatataa tattaatcat
     1981 gtttttagtt tattatattc tataatagtg gtcatgttga ctattgtagt tttattaaga
     2041 atagttggtt tatataaata tatgcaaaga tagcttgttg tttactttac ttattagtat
     2101 ataggacaag atactctaat aaatttgcct attttaatat cttttgtact aaaaccaact
     2161 aaagtttcct ttattatttc gttttgaata atacaaagca taatccgatt gtttgtataa
     2221 agactggaaa gtatcagagt tattttctaa tttagatata cctatagaac aagaattact
     2281 ctctaggcat aattctaaaa ccttctttgc cttattttca acattctctt ttgattgatt
     2341 actatattca aaaacagcaa attcatctcc accaagtctt ccaataatag catcatttac
     2401 gaatacttta gtcaaaatat ttgaaaaatg tattagtatt tcatctcctc gaacatgacc
     2461 aaagatatca tttacctgtt taaaattatc taaatcaaag atgtaaaagt atccatgtga
     2521 aacagtttta agcatttctt ttatgtgtac ttcaaaaaaa tgtctattgt atagtccagt
     2581 cataaggtct ttttctgatt ttagtttaaa ttcatttgcc attgctagtg cttcttgtgc
     2641 tttttcagtt attgttttag ggtaatactc atctttcatt tggtcaaagt ttggaataga
     2701 atggtgaaca tggataagtt taatcatatc atctgtaact ctgtatacca tagtaaatcg
     2761 agtatccatt tctacataga tatctttttc taggtaatct ttttctatta cccaaagagt
     2821 accatatact ataaaagctt cgtctgtaac tttggtacat gaataccact catctataat
     2881 ctcaaagtct atttgctctg attctgtttg gtctttttca agacctataa taaattcttc
     2941 taatgtttcg taaaattcat gttttcctgt tccaattaat gcaaggttgt catctaataa
     3001 ggaaataata aatttaaggt tttcttcttc tggatttaaa atataaatat gccaaatttt
     3061 ttttgataaa tctcttaatt gtttttgtat tgtattattt tccatagatt acccccttaa
     3121 gatttacagt ttatatttta atgtattata agttgaactc ttattacaat attattataa
     3181 tatagaacta aagaaattac atcctgtata agtaaaagta tatcttttat aataaaaata
     3241 ttacagtaaa ataaccagtt ttaatatacg tattatattt ttaatttata aacgaatatt
     3301 ataaatgaaa tttaaattca accctgaaga aataagctaa atgtattagt atataaatcc
     3361 atacttgaac ataaataacc tttatgatat gatatcaata ataatattat gagaggagtg
     3421 ccttgatgtc ggagttatat aatggtaacc aacaattaaa tagttggaga cacatatatt
     3481 ctgtataatc agaaatttca agtgtatttt taatacattt aatttttgtg tcttattctg
     3541 cctttataga taactgacat atgacaacaa atagtattta aaatatatat tactttgtta
     3601 aattatggtg tagtaggctt taatctgcta cacttttatt aattttgtat agtattattt
     3661 tgataactat tttgaaatag agagcattaa tttttctagt ccacaggagt tttctgtttt
     3721 tgtgctgtta aatataagca cagagaaagg cagatatata cctgtggact ttttttatat
     3781 aaattatgaa atatggaggt agttaaaatg agtataattg aacttaatga actgactaaa
     3841 tcttatagaa gatttgagaa aaaggcagga tttatgggga gtgtaaagtc tttatttaat
     3901 aggaaatata cagaaaaaac agctatatcg tcatttgatt taaagataga agaaggagaa
     3961 tttgttggat taataggtca aaatggagct ggcaaaacta ctcttattaa gatgttaact
     4021 ggtattattg caccgtcttc tggagaaata tctgtaatgg gttattaccc taacaaatta
     4081 gaaaataatt ttaaaaaaca atatgctgtt gttatgggcc aaaaaagtca actattcttt
     4141 gaattgacac cagcagatac gtttctttta tttaaagaat tatatgatat atctaatgaa
     4201 gaatataaga aaaacttgga ttactttatc gaattatttg aggtatctga ctatttaaat
     4261 gttcaagtta ggacgctttc tttaggagaa agaatgaaaa tggaactaat agtagccctt
     4321 ctacataatc caaaaatatt atttttggat gaaccaacta taggcttaga tgctatagcc
     4381 caaaaacaaa ttaggtattt cctaaaagaa gtaaatgaaa caaaaggaac tactattata
     4441 cttacttctc attacatgga agatattaag agtctctgta gtagaagtat tgttgtagaa
     4501 aatagccgta aaatatatga tggttctaca gagaaactat ttaattattt tcaaaaatat
     4561 aaaaaaataa cagttagttt ttccaatgaa actaacattg tatttccaaa agattgtaaa
     4621 gttgttgaac aaacaaatca taagatatca gtaattgtgc caaaagaaag aggaaatgaa
     4681 acgattaaat ggatttttga taaatatgat gttaaagatg taatgattga agaagaagat
     4741 ataggtagta ttgtagagag gatatataag aaaggaagtg atgagtatta tgagcaacta
     4801 taatgaagca agttcaaatg attcaatgtt ttataaattt aaacagagta ttagaaggta
     4861 ttatgaagta gccaaaatta actttaaagt gcaacttatc tggagatttg atgtggtggt
     4921 taatatggtg tttacgattg taaaaatttt atttgcttat attttatgga aagcaatttt
     4981 tggtgaaaat aaaatagtct ctggttttac atttaactct atgttatctt actatataat
     5041 aagctcattt ttagcaggta ttgatatgtc aaagagtacc agtgaagaga tatgtaatcg
     5101 tgtaaaattg ggcacctttt caaagtacat gattattcca attaatgttc aaaattattt
     5161 tattgctaaa aattttggaa tgacatgttt ttattttgta tttaatatga tttctgcatt
     5221 tgtatggatt tttatattta aaatagattt tgcctttaca tcaaatccag ttcaaatatt
     5281 aatttcaatt ttgatgattt ttctagggtt tatttttatg atgaaactcc acttctttat
     5341 tgggattttg tcatttaaat ttcttgagat tagttttttt aaaatgataa ttgaaaatat
     5401 tgttgaattt attacaggaa caatgatacc acttgtactc ttaccagaaa ctatagtaac
     5461 agttatgaag gtatttccat tttattatac aacatatttg ccatctatgt tactaatagg
     5521 tagaaacagc aatgaggcaa tacaaggatt aattattcta gcattatggg ttttattttt
     5581 ttctgtagta aataaagtta tgtataaaag aatgagaaca ttatttgatg gggtgggtat
     5641 atgatgaaaa aaaatttgaa aataatattt atgttattaa aaatgaaatt atcaaagatg
     5701 atggtattta gattcgattt ttttggagca ttttttgtag atagttcatt atttatatta
     5761 caacttctaa tgtttaatag tatatattct catgtagact caataggggg atggcaacaa
     5821 ggagagatgt taatatttat aggaactttc tcattaataa atgcgataaa tatgacgatt
     5881 ttcttttttg gtatttatga tattccacga aaaatccaag aaagagaatt agactactat
     5941 attacaaaac cattagaccc actatttagg ttaacatttg aaaatattaa tattggttca
     6001 tcaccattaa ttctagcaag tatacttatt gttttatatg gaatatctga gttaaactca
     6061 gagataactt tagttatgat aataatatat attttcatgg tgttattgat gacattactt
     6121 ttttatgatg tatgtatcat acttagaaca gtttcatttt ttgttatatc atcttctgcg
     6181 ataatgagat tagaagataa tttattgcca atgaatatga aaattcctgg tgtaatatat
     6241 gaaggtattt ttaaagtatt attctattta atcttaccat atggaattat gtcgacaatt
     6301 ccgactcaaa ttttggctgg gacaataaca attaatggat taatatactc aattttaatt
     6361 gtatttttgt ttacattgtt tatgtggaga ttttggaagt taggtatgag acattataaa
     6421 agtgcaagta gttaataaat tagtaaaaat agacaatgta taactatata taaatacgtt
     6481 tcatatgaca ctaaactatg tgaacaagta tttgcgctaa aggcactgaa agctataaaa
     6541 tgattaaagt aaggatttaa aagtttcttt agtagaaata taattgtgta aaaaagcaag
     6601 attaattaaa actatatcca aataattaga tataattctt atgcagaaag gaggtctgta
     6661 aatgtctaat tgtttggaat atattttaaa aataattaat gtacagaaaa ataaacaaaa
     6721 tgagtgatgt atattattca gaaaatcatg aacaagataa tctttatgag caagataatt
     6781 tactggcaca aagatttaat ttatttactc tattgaagtt tgcatttcct actattttta
     6841 tgatgatttt tatggggttg tatacaatcg tagatactat atttgtatca agatttatta
     6901 atacaaatgc tctatcagca attaatattg tatgtccaat tattaatctt atagttggaa
     6961 ttggaactat gcttgccact ggaggaagtg ctattgtagc acgtaagatg ggaaatggtg
     7021 atattgaggg agcaaataaa gctttttctt taatcgtatt atcaggagtt gtaattgggg
     7081 taataattac tggtataggt attgtattta tggattatat cattatatgg ttaggagcaa
     7141 gtgaagtttt aattaaatat tgtcgttcat atctttttat tatcttggtt tttgcttctg
     7201 caaatatact acaagtatta tttcaatgtt tatttgtaac agctggaaag cctcaatttg
     7261 gattatggct agtgttaggt gctgggtgtg ctaatataat tcttgactat gtgttcattg
     7321 ttctgttaca aatgggtatt agtggagcag cacttgcaac tggagtagga tacctaattc
     7381 ctaccatttc aggtatggtt ttcttttcaa aaaataaagg gttacttaaa ttttgtatac
     7441 ctagactaga taaaagaata ttatttgaag cttgttttaa tggttcatct gaaatggtgg
     7501 gtcaactatc aacagcagtt actacttttt tatttaatgc tatgatgatg aaattaattg
     7561 gtgaagatgg ggttgcagct attacaataa ttatttattc tcaatttctt ttaacttcgc
     7621 tttatatagg tttctcaatg ggtgttgcac caataataag ttataactat ggaagtgaaa
     7681 ataatacgca gttaaatcat gtttttaaga tttgtatatt atttatttca gtagcatctt
     7741 tattggtatt tatcttttct atatcatgtt caccaatcat tgtagagatt ttttctcaaa
     7801 gaggaagccg agtttatgaa attgcaaggt ccggatttta tatattttct tttagtttta
     7861 ttttttgtgg atataatatt tttacctcat ctatgtttac agcattatca aatggaaagg
     7921 tgtctgcaat attatctttt ctacgtacat ttggatttat aactataggt ttattgatat
     7981 tgccaaatat attaaatata aatgggatat ggttagcagt accaagtgct gagattttaa
     8041 ccttattttt aactgttttt tgtatattta agtataagaa tagatataaa tatatgtaga
     8101 tattaaataa atagcttaga tagatatttt atgttttaat aagtaattca attagtggta
     8161 gatatatact attgaaaatt aaagttataa ttgatataaa gaattaataa aaataacata
     8221 taaaagttta aaagggagtt gaacttaaag tttgattaga aaaagtcaat tttaagacaa
     8281 ctccttttat tttactattg tattaatctg tatctgtaag tgtattagtc taatattata
     8341 aactttggat gaaattaatt ttctataggt tttaatgtat catacacttt attttaaaga
     8401 aacttatctt aattttcata gtgattgaat ttgtctatta gagatgttac tattagtaat
     8461 gtaaaagcaa gagtagtgaa tataaaaaac aagatagtga tactgcaaga ttagaatata
     8521 ataaattttt atggagagat ttcaatatgg atacatgttt taataaaaaa atattacttg
     8581 ttgatgatga aaaggatata gtagatttaa ttgaagaagt actcataaat gatggtttta
     8641 aaaacattat taaagcttat aatggtctag atgctatttc gctgtgtaag atagcatgtc
     8701 cagatgttgt cattcttgat ataatgttac cagatataga tggtatagaa gtgtgtaaaa
     8761 aaattcgaga gttttcatac tgttctattc tttttttatc atcaaaaaat gatgatattg
     8821 acaaaatact aggtcttagt agtgggggag atgactatat aacaaagcca ttcagtccaa
     8881 gagaaattgc ttttcgagtt aaagcgcaat tacgtagaca acagtatcaa agcattgtac
     8941 catcagatag tgaagttatt aaaataggag atattactat tgatatagaa ggaaaccgtg
     9001 tatataaaga tagaaatgag attgagttaa ctggaaggga gtatcatctt ttaagctaca
     9061 tggctcaaaa tgtaaataaa attataggaa aagaaagact ctatgaacaa gtatggggag
     9121 tatatagtag tatttgtgac aatacaatta tggtgcatat acgacatata agagaaaaaa
     9181 ttgaagataa cccctcaaat cctaaaatac ttataacagt taaaggacta ggatataaac
     9241 ttgtgaatag aatcgattag aatagatttt aatattaatg aagatatctc aagttgaatt
     9301 tttagttaat gaggcattct tttatgggtg aaatcaaatg tattttcgtt atctcaacaa
     9361 tgaagaagag gtctatctct attttgagat agcctcatta agtttatatc ctggtaatgt
     9421 actttatgtt ctaataaaat ttttttagtt tatttgcagt gaaacattaa ctagaacatt
     9481 aaaatttgca ttgaaatacc aattaaataa tattgtatat tgtgtataaa cttatactaa
     9541 ttgtttttct atattaaata ctttatcaat ccaaccttta taaaattttt cttcatggga
     9601 aactaggatt atacttcctc taaactgttt caaagcattt tgtaaagcat cctttgtctc
     9661 tgcatcaaag tgattggttg gttcatccag aataagaaaa ttacaaggag ataacatcat
     9721 acaacataat ttaaccttag attgttcacc accacttaaa gtagatacac tccttgaaac
     9781 atgctcttcc ttcacaccac atctggctag atgacgtcta atctcttttg tatttaattt
     9841 tggatattta tctgctacta tttgaagtgg ggttttatca ggattttccc attttaaatc
     9901 ttgctcataa taaccaattt taacttgttc tgaaaactgg aaatctccag atatacgagg
     9961 aatatctttg actaatgtct ttaacaaggt agatttaccg attccattaa aaccagttat
    10021 aactattttt tgacctccat caacagagaa atttaatttt ggaagtaaag aatagtagta
    10081 tccaacttca agatttgtta tagtaagagc attttgagcc gatatttcaa tttctgaaaa
    10141 ttgaatattt ggttttcctg taaaactagg aggagctatt ctttctattt tatccaattg
    10201 tttttgtctt cctcttgcaa tttttgaatt tactccagct ttatttttac gtataaatgt
    10261 ttcttctttt tctattttct tttgttgtgc ttgatacctt ctaatataat cttctcttaa
    10321 atgtgatttt tgtttaagga actctgaata ttttccatga tatttcttaa tcatgccaaa
    10381 ttctatatca agaatacctg tagaaatttt ttctaaaaag tcaaaatcgt gggaaactac
    10441 aataaatgcc ccattaaatg tatttaaata atttgagagc cattcaacat gctctttatc
    10501 taaaaagttt gttggttcgt ctaataatag aacatcatgg ttagaaagta gtaattttgc
    10561 taaaattacc tttgcccttt gaccaccact aagctctcta acaacacgat tcattcctat
    10621 tgaatctaat ccaagaccat ttgctatttt atttatttca ttatctatag aatagaaatt
    10681 acttgctatt agttgttcct gataatctga tgctttgatt agatattgct cattttcaga
    10741 tatagcactt ttttgataga gtaaattcat ttccttttct attttataaa gttcttcaaa
    10801 tgcagtgtgt aaatagagtg atattgttgt atctctattg atttctgcat attggtctaa
    10861 atggcctata ttgatattag gttgccactt gatttctcct gaatctggaa caacttctcc
    10921 caataaaatt ttaattaatg tacttttacc tgtaccattt tggccaacta cacctatatg
    10981 ttcacctttg aataaatcaa agttagcttt ctcatacagt gatttatcca taaaacattg
    11041 tgataaatta gttactttta ataaactcat aattcaaact ccttttcttt tattaaaaaa
    11101 tttatggaaa taaaaaaagc agaggaatgg cattgtaaaa tgccgacctc tgcttttgga
    11161 aaaacaaaag gtcatagaca agcattgttg tctatgacca gaaaatctat attatctatc
    11221 ggcaaatagg taattttaaa taagcatagc caaaaaacta tactaaacct atatttgccg
    11281 atgaagatag aaagctttaa gctttcagat taacctctac acaacgcttc atcgtataca
    11341 tttgtgtaga gttaagtttt ctaacaagtt taactaattc tatataaatc ataattattc
    11401 acatcctaat cttagtatta attctagttt atctgtattt aaattgtact tttgtacttt
    11461 gtggattatc ttatttattt aaaatatgtt ttattaatta tagcttaagt tatgttatca
    11521 taatacttag ttatagtcaa taagaaagtt atatttacta ttattatatt aatttagtaa
    11581 atctatcatt acaaagttat ttgaagactc gctatcaata aaattttata gtctataaga
    11641 aaattaagat tatgagtttt ataattttga tatagggata ctattttcat aaatattaca
    11701 acatgtagaa gtatatgtct attattgttg aa
//
LOCUS       DAEMGG010000121        11729 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEMGG010000121 DAEMGG010000000
VERSION     DAEMGG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.122"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(782..1099)
                     /locus_tag="KPK24_003048"
     CDS             complement(782..1099)
                     /locus_tag="KPK24_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300700.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIGLFTKRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
     gene            1262..1435
                     /locus_tag="KPK24_003049"
     CDS             1262..1435
                     /locus_tag="KPK24_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300701.1"
                     /translation="MNDATKVSIKNETNNIMNLCNLLSWNALLQNNKINAETIAKSRY
                     FIIILSPIDFYRL"
     gene            complement(1409..1765)
                     /locus_tag="KPK24_003050"
                     /pseudo
     CDS             complement(1409..1765)
                     /locus_tag="KPK24_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2157..2546
                     /locus_tag="KPK24_003051"
     CDS             2157..2546
                     /locus_tag="KPK24_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2300702.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEGDIDD"
     gene            2866..3375
                     /gene="vanZ1"
                     /locus_tag="KPK24_003052"
     CDS             2866..3375
                     /gene="vanZ1"
                     /locus_tag="KPK24_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBG2300703.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(3477..3671)
                     /locus_tag="KPK24_003053"
     CDS             complement(3477..3671)
                     /locus_tag="KPK24_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBG2300704.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(4187..4579)
                     /locus_tag="KPK24_003054"
     CDS             complement(4187..4579)
                     /locus_tag="KPK24_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2300705.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            5146..5508
                     /locus_tag="KPK24_003055"
     CDS             5146..5508
                     /locus_tag="KPK24_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300706.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            5492..6199
                     /locus_tag="KPK24_003056"
     CDS             5492..6199
                     /locus_tag="KPK24_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300707.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDISNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEIINQFKNESENIRNQEVVEFTEEDEEELKQNIIENIK
                     KNFDEKNLVQVYKYRGV"
     gene            6219..6638
                     /locus_tag="KPK24_003057"
     CDS             6219..6638
                     /locus_tag="KPK24_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300708.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEVYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            6628..7152
                     /locus_tag="KPK24_003058"
     CDS             6628..7152
                     /locus_tag="KPK24_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300709.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKCSQKYE
                     TDFKIKVWNFSESI"
     gene            7464..8021
                     /gene="efp"
                     /locus_tag="KPK24_003059"
     CDS             7464..8021
                     /gene="efp"
                     /locus_tag="KPK24_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG2300710.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            8113..8568
                     /locus_tag="KPK24_003060"
     CDS             8113..8568
                     /locus_tag="KPK24_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2300711.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            8731..9441
                     /locus_tag="KPK24_003061"
     CDS             8731..9441
                     /locus_tag="KPK24_003061"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG2300712.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            9495..10565
                     /locus_tag="KPK24_003062"
     CDS             9495..10565
                     /locus_tag="KPK24_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2300713.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            10593..>11729
                     /locus_tag="KPK24_003063"
     CDS             10593..>11729
                     /locus_tag="KPK24_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2300714.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNN"
BASE COUNT         4815 a         1094 c         1836 g         3984 t
ORIGIN      
        1 ttatatatat tataacaatt atagcaaatt tttagggtat aatttttaaa attatattct
       61 actatcaaaa gatatgaata aatgattgta tatattatat caatttttat atttcatagt
      121 ccattttttc atcacatttt cgcttgtcaa gaataatatc tttaagagtg aagcgtttga
      181 tgtaaatctg gactttgttt ttgagacatt tttgaaatat ttattaaatc tttcgattat
      241 acttgtaata tatattatag atattactta ttttagtaaa actaaaggat gatgctacaa
      301 cattccagtt atttcccaaa attttaattt ctgaagtata ctgtttacat tatttttaat
      361 ctattttatc taaagtcgat aaggcttctt attcattaac tgacctatat acagatttaa
      421 aattacttat taattctttt gagtccttgt atgaaacata tttaaagtat ttcttatcta
      481 atgtattaaa catttttgta ttacagaatg aggataactt gtagctattg tctctttgaa
      541 ataagtcatc tgtgtttact cctaaaacta cttatatctg tagattttat aagttctcca
      601 tcttatataa tttctttgat taattgtttt cttaaattat tcataaaaaa atctccgtta
      661 cgatatttga ttatatttaa atcattgcta taaagaagac ttttattaac tttgaaaaac
      721 aaattatttt aattgctggt gattagttgc taccttcttt tgatattaat tctattagat
      781 tttatatagt tatatatctc tttctaacta agtaatataa tattactgtt atagtagcta
      841 ctaatattat tgctattatt gtaatatcta atctttttgt aaataatcct attgctatat
      901 attcaaaacc tgttacatat cgataaagta cttctagttt aatagttctt tttctatcta
      961 tatttccata ttttgattct aatttattaa aatatttttc attacaaaaa ccaattttaa
     1021 tttcaattaa agatattatt gtagttaata ttataattcc caatcccata agtataagat
     1081 aaatattcct aaaattcata gaagatacct cctttataat ataatcatca actcatgttg
     1141 tattaatttt cctaatcaaa gtataacagt aacacaagta taaagagtat ctaaaatatt
     1201 acaaatatta aatgagaaaa agatggtaag ttaaccccac catcttattt tttatctact
     1261 aatgaatgat gctacaaaag ttagtataaa aaatgaaaca aacaatataa tgaacttgtg
     1321 taatttactt agttggaatg ctttattaca aaataataaa attaatgctg aaacaattgc
     1381 aaaaagtaga tattttataa tcattttatc acctattgat ttctacagat tgtaagtagt
     1441 atttggttac acaagcacct cctttattat aacgtatttt ttaaatttaa agatttttcc
     1501 agatgttata tttgactcaa ggtacattgt aattaatcta actataagtg gcaagtactg
     1561 tcctatacca ggaatagcac cagccaaaag tcctgaaatc cctataccag catcagttaa
     1621 agttaatgca actcctttag aaagatataa attaaaacct attctagccc aaactatttt
     1681 atttatttca gcttttctat aatataattc aagtagtgtc aatatcgttc ttcttaaggt
     1741 ttttaattag cttatatatt gttattgtat aatataatta ttaaaaatta actatattaa
     1801 atattaataa aaaatatagt aaaacttaat aatacataca ataaaactta atataaatta
     1861 agtatatgaa ataaatatcc tgtatttaag cttatagatt ttattaaata taatacatat
     1921 tataataata aaacgaacta aaattatcac actaaagcat aaaacatact ctaatcatgt
     1981 tagataaatt tttgaatata taatccaaaa gtactctcta aagtagagta cttctttttt
     2041 attaattatt tttatataga ataaattgga atttatattt attccaatct aattgattat
     2101 atacacttat tgttataaag tttagttata cataaattgg taagaggtgt aaactaatga
     2161 agattaataa attaccacat gcagaactaa aactaatgaa gtatatttgg ggagtagatg
     2221 atgtattagc ctcaagagat atcatagaag acatgaagtt gaaatacgat tggaaaaaga
     2281 gcacaacatt gacattttta aaaaatttag tagataaggg ctttttaact actgataagg
     2341 tagatagatg tacgcattat actatagcta taaaagagaa agattactta aaagttgaga
     2401 caaagagttt ttttagtttt atgcataaca attcatttaa gagttttatt tcagcattac
     2461 atgatgatga agttttagat agtaagagct tggacaagtt agaagaatac tttaaaaatt
     2521 tgaaagaagg agatattgat gactaataaa gttatcagta tctttttagt ttgagacaat
     2581 tgagagtagc gttttgattt aatgttagtt ttatgtgatg gtagaacatt atataaagaa
     2641 aagtgattat ttccttagtg tttgatagta aaaaatatag agaataatac caattaaaat
     2701 ttaaagatat atgaaatact acaatagctt aaatcatgta aatatgataa agatattgaa
     2761 attataaaag gaatataatt ttagaagaaa tagttgacag ataaattacc ttactatatc
     2821 attgtattaa taaaataata caatgatata gtaaggaggt aataaatgaa atctagaaaa
     2881 cataatatta caaaaggatt atttatagtt tacataatta ttttaacatg gataatacta
     2941 ttcaagttgc aatttgatat ttcatccctt gaaaccatga atcttagaag cataaactta
     3001 gtaccttttg ctgggtcact tataattaat aatagagtag atatatcaga gattatatta
     3061 aatgtggcta tatttgtacc atttgggata tatgtgtgta tgctaaaaga agaatggagc
     3121 tttataaaaa aagttatacc tatttttata actagtttgg catttgaaac acttcaatat
     3181 atatttgcac taggtgcaag tgatataacg gatttgatag gaaacacact aggaggaatc
     3241 ataggaatag ctgtttttat gctattatcg aaaatattta agaataatac cataaaaatt
     3301 atcaatgtat tggcattgat tgtgacaatt attgttgtat tatttttagg attagttatt
     3361 tttgcaaact tatagaaata agtatatatg tttagcttaa aatagaacgt aagatacagt
     3421 tacatataaa attatttaca cataaaaagc acctatacag gtgctttcct aaattattaa
     3481 agaattattg tatcttgaaa ctcttgtatg ttcaattctt gattttctct tgttttttct
     3541 aataaatcta acgcacaatt tatacaaact ttaatttctg cattggtatt tttctcataa
     3601 taagttatat atttatttct atatttatct atttcttttc cacaacgact acacattaca
     3661 attacgctca tatcttagtt cctccatctc taattattct taatatattc aatagtatca
     3721 cttttctgaa aataatgatt gttaaaactg tatcaaattt agatttactt tttattataa
     3781 tgcattttaa taaaaaaata aaggttatag gtaaaatatt tatagaaaaa agtatattat
     3841 tgaatagatt ataaaatttt attatttttt agcagtatta aatacacatt aatatctata
     3901 aaatgacaaa ataaaaaata ctatttaaat agatattaaa aaattgatac caagtatttg
     3961 tttgctatat gaataacaat ttatttagga gctttacttg gacactgtat aataataaag
     4021 taattgctaa aaacgtttga tgatttggat gtatttttag aaattagaaa aatatatact
     4081 tactgactaa tgataatctt atatgtattt tagtttgagt gatttaagaa aagtgttttg
     4141 aatggggatt attttataga gtaaataaat atataatagt agaaaattac atataaagag
     4201 tagtatttat ttcattacct tctttatctg ttccgatagg gtcttgtaga gatacttgag
     4261 ttttattttt tttgttagat ctaatattca ttaaaatctc attttcaata catctagaag
     4321 catatgttgc taatcgagtt cctttatcta tgttataagt ttctatagct ttaattaatc
     4381 ctattgtacc tatggatatt aaatcatcct gatcttccgt tgaattatta tatttctttg
     4441 ctatataagc tactaatctc atgtttcttt caattaatgt atcttttgca cttttatcgt
     4501 tttctatttt aaatttagtt aaatactcaa tctcttcttc aggggttaag ggtttttcaa
     4561 aagatttaag agctgccaat aggtcacccc cttttcaacg gtcactttaa attatatgaa
     4621 ggagacttat ccaagttgac tgttatctaa taaaataata tgcatatttt tagaatttaa
     4681 aacatatatt tgcaaacttt tgaatggaat atgacataaa gagtgggaat ttaaatttaa
     4741 gtttttgttg tatatatatg aaacaaggcg aagaaattat tgatttataa agtataaatt
     4801 ggaattgagg tgtcaataaa tataaaagga aatagtattt atatatgttt tggtgttgaa
     4861 gtaaaagatt aatgcaaagc attagagtct ataaaaatat gaatatagga ggtgtaattt
     4921 tacaatactt aaaaaagtaa atgtaattaa ttgtttgtat aaagttgatt acaagcaaaa
     4981 gtatcagtca tagttaatac tgttaaggat gaaggtggtg tgttaattta tatatgtatg
     5041 ggaagaaatt ttgattacaa tgaaaaaact tacaaaagat atttacgaaa aaacttatag
     5101 ttaaatgttt atatataaaa tattatttag gggttgtttt tattattgaa aataagaaag
     5161 agtggtttta tatcaattga atgtataata agtattgcta tattatatgt ggctgtttat
     5221 ttagtttcta catcattgta taattgttat agttttatca gtagaaatat atctgacaga
     5281 gaaatgttaa gtacagcaaa aaaatatata gaagatgaga agtatagaat acaaaatagt
     5341 aagtatgagt taattgaaga taagatagaa aaaaattaca taaatggata tgaaattaac
     5401 agtagaatag agcaaatttt agattattat caatgctatg aaataaatat agagataaaa
     5461 aatgaattta aaaaactgag gtttaatagc tatgttacta gaaaataaag gctctatatt
     5521 aataataaca ttgataatat tttctattat aagtactgtg tgcattatgt gtataggttt
     5581 aatttataca cataataata tgtttgagtt aggatacaaa gacattcaac taaaagaaat
     5641 ttctttaagt ggaatagaaa tatcagtaag taatgtactt tcttgtgttg aggaagctat
     5701 tgatatatct aataatgaag acgaatttag agaatatttc ttaggaaata atatgattga
     5761 atgtataaaa aaaataaaag atacatctta tagtagttta gaagaacttt ctttaaaagt
     5821 agaaaataat actatttatg acagaggaga tttttttgaa tttgatatag aaagtaatgt
     5881 tagagaaaaa gaatttttaa aagcaataaa agtaagagtg aaaatcaaaa atccatggat
     5941 aggaattgat attgaagatt atatggataa atctaataac aataaaattg atgacaaaac
     6001 attcaataat tataaagcat ctgaaaataa tgaaattata aatcaattta aaaatgaaag
     6061 tgaaaatata aggaatcaag aggttgttga atttacagaa gaagatgaag aagaacttaa
     6121 acaaaacata attgaaaata taaagaagaa ttttgatgaa aaaaacctag tacaggtata
     6181 taagtatagg ggtgtataac tttgaaaaaa gaaggaatgt gactgtaata gaattaatag
     6241 tatctatcag tataatactc ttgatagtta cattatcttt tccaaaagat aatcttgaaa
     6301 atcataaaat aaatttattt gcacgtcaac tttgttcaga tataaggtat gttaggcgaa
     6361 ttaatatgtt tagcaactac aatacatata tatattatat aaatcaaggt aaacacgaag
     6421 gttatgtatt gagaaaagat ggtaaaaaca ttaaatcaat aatgctacct aaaaatgcaa
     6481 aaatagctag gagtattgaa ataataaagt ttaaatctga tgggtcgcct caaaaggcgt
     6541 ctacaataga ggtatataat aatagattaa gaaaaactat aacaataaca cctgtaagtg
     6601 gaagagtact tctgaaagag ggaaaatatg aaacataaat atggttactt gcttttggaa
     6661 agtgtcgtat cattgtcttc aatggtgata attatactag ttttatattc tatatttttg
     6721 tctacaataa acttaaagtt aaaagtagaa gataagatag aactacaaca acaaagtctt
     6781 gaaatcataa aatctatgga aggaattatt agcaattcta tgggaataat gaatgtatct
     6841 aattatgaag aaacatttaa aaagacaaca tctataaaat gtagatattt agatgaaaat
     6901 aataatgaag aaggcataag taataaagaa attatattga atgaaagacg aaataagtta
     6961 tttgtaaatt ctttaaatgg cgaaagtagt caagcaggtg gttatgaaat tggagattat
     7021 gtagatgaaa tgtatgtatc aattactaat aatggtcaat acgttaatat taaattgaaa
     7081 ttatcaaaat gtagtcaaaa atatgagact gatttcaaaa taaaagtatg gaattttagt
     7141 gaaagtatat aaaaatacat aaatttaaga gaaagaaatg acatcaataa gtcatataaa
     7201 atataattat tgagaactat ataactaaat aatatcaata tgtaaagtgt atagattaag
     7261 aatatagagg ataataataa aaatatagaa taagtaattt gccaaatact tgtttatatt
     7321 gtataactta tgatattatg atattagtaa aaatttgtaa tcctgacttg acagaattca
     7381 agtaagagat ataattttaa aggcatagta atgtaaaatt tactaaaaaa taagaaattt
     7441 attttaatat ggaggaacaa aaaatggtat cagcaggtga ttttagaaaa ggtgttacat
     7501 ttgaaaaaga tggacaacca tgtttagtag ttgattttca acacgttaag ccaggtaaag
     7561 gagctgcttt cgttagaact aaatacaaaa acttaaaaac aggagctata agagaagaaa
     7621 gtttcaatcc aagtgaaaaa ttccctaaag ctgttataga tacaagacaa atgcaatatc
     7681 tatacaatga tggtgagtta tattatttta tggatcaaga aaattttgaa caaataccat
     7741 taaactatga gcaagttgaa gatgcaatta agttcttaaa agaaaatgaa gttgctacaa
     7801 taagatttta ccaaggacaa ccattccaag tggaagcgcc aaactttgca gagctagaag
     7861 ttacagatac agagccaggc ataaaaggtg atacagcaag taatgtaact aaagcagcta
     7921 cagttgaaac tggagcagtt gttcaagttc cattatttat aaatactgga gataaagtaa
     7981 agatagatac tagaacaggt gaatatttat caagagtata aaaatttatg tatataaaag
     8041 cttatatgtc ttaataatat aagatatatg agcttttagt ttatatataa aaaagaaagc
     8101 gaggggtgtt acatgaaaca tttatatgaa gaagttgcat acttaaaagg cctagcagaa
     8161 ggactagaaa taagtgctga aagtaaagaa gggaaaatga tacataaaat agttgatgca
     8221 ttagaagtat ttgcagatgc tattgtaaca ttggatgaag aacaagaaga acttcaagat
     8281 tttgttgaat ctatagatga agatttagca gatttagaag aagatattgc tgacatggaa
     8341 gaagatcttt atgaagaaga tgacgatgag gatgatgagg acttcagcta tatagaaatg
     8401 gaatgcccaa attgtggaga gttagtagaa atagacgaag atttgttata tgatgatgaa
     8461 gtagatgttg tttgtcctga ttgtaaggct gtgatattat catcagaaga tgattgtgat
     8521 gatgattgta catgtggtgg ttgttcaagt tgtgatgata gagagtaaaa attcataaga
     8581 attaaaactg cctatgatgc ctatgagata ttttgcttca taggtagttt tatttttatg
     8641 aggaagggta ttatgtattt tggttttgca atacttggac aaaaatagta cactagaatt
     8701 tatagaatag gattttggag ggatgtaaat atgaaaataa gtaaaaaatt actagataat
     8761 atacaaagat ttgaaaatgt tataaattat aaatttaaaa ataaagaata tatactagaa
     8821 gctcttactc atagttcata ttctaatgaa aataaaaaat ataattttaa cgaaagattg
     8881 gaatttttag gggattcggt tttaggcata gtgataagtg attatttatt taatgaagaa
     8941 gctaatctgc ccgaagggga actaactaaa cttagagcaa atatagtctg tgaagattca
     9001 ttaagtgaag tagctaatga tataaattta ggtattcaca tgttattagg tagaggagaa
     9061 gaagcaacag gaggaagaca taggacttca atattagcag acgcatttga agctgtaata
     9121 gctgctatat atcttgatgg aggttttgaa agtgcaagac agtttatact tcatcatatg
     9181 gaaaatataa tttatgattc aagaaaagga aatatattta gagactacaa aacgcatctt
     9241 caagaggtgt tacaagggaa tggagaaaat aatatctggt acaggcttat agaagaaaaa
     9301 ggaccagacc ataataaaag atttgtaatg gaagttggaa ttaatgacga tgtacttggt
     9361 ataggagaag gcaagagtaa aaaagaagct gaacagttag ctgctaaaat agctttaaag
     9421 aaaaaattat gggaaaaata aatataatat gtaaaaatat aaaaagaaat atttatatta
     9481 gaaggatgtt gtagatgaaa aagagaatta tacctatatt tgttcctcac aaaggatgtc
     9541 ctcatgattg catattttgt aatcaaaaaa aaataacagg tgtgagtact gatgttacta
     9601 gtgaagatgc aagaaatata attgaggaat gtttagagac tatagataaa gatgcagatg
     9661 tagaaatagc attttttggt ggtagcttta ctgctataga tattgatata caaaaaagtt
     9721 tattatcagt tgctaaagaa tatgtagaaa aaggtcttgt aaaagatatt agaatgtcta
     9781 caagaccaga ttgtattgat gaagatatat taagtatgtt aaaagaatat aagacaagta
     9841 taattgaact aggagtacaa tccttagatg aaaaagtatt attagatagt ataagaggtc
     9901 atcaatctga aattgtatac aagagttcaa agatgataaa aaatagtgga atcaaacttg
     9961 gattacagat gatgattggt cttccagctg acacagaaga aaagtgtata tttacagcaa
    10021 aaaaatttgt agagctaaaa ccagattgtg ttagagtcta tccaactttg gtagtaaaag
    10081 atacaggtct tgaaaagtta atggaacaaa ataaatataa tccatttacc cttgaagaga
    10141 gtgtaaatat agtaaaaaaa gttcttgtac tgttttatgt taataatatc aatgtaataa
    10201 gagttgggct tcaagctact gatgatatac aaattggaaa agctgtttta gcaggaccat
    10261 atcatcctgc atttagagag ttggtagaag cagatatgat aaaagattat ttagaatttg
    10321 ttattttgca aaataaaaat ataaagcaaa tgttggtcaa atctaataaa aagaatattt
    10381 ctaaaatcat aggaaataaa aaaacaaatg ttaaatatat ggaagaaaaa tttggtgtgc
    10441 ttttaaaaac acaagaatca gatttagata taaatcaatt agaaattgtg ttagatggga
    10501 aaagtttaat tagtgctaat atgagagaca ttcatagaaa attgtatgac atttatgacc
    10561 tttagtttta ataatgaagg gagagaaaag atttgtattt aaaaagacta gagttaaagg
    10621 gatttaaatc ctttccagta aaaacagata ttatttttaa agaaggaata acagccatag
    10681 taggaccaaa tgggagtgga aaaagtaata tatctgatgc tgtaagatgg gtattaggag
    10741 aacagagtat taagagtctt agaggtgata aactcgaaga tgtaatattt gcaggtacag
    10801 atacaaaaaa acctatgaat tattgtgaag tggctcttac tattgataat tctgaaaatc
    10861 aattggaatt agattttaca gaagtaacta taagaagaag ggcttataga aatggtgaaa
    10921 gtgaattttt tctaaataat aaaagttgta gattaaaaga tataaaagaa gtctttttag
    10981 atactggtat tggaaaagat ggttattcaa tcatagaaca aggaaaagta gatgaaatat
    11041 taagtaataa tcctttaagc agaagaaaag tttttgatga agcttgtggc atatcaaaat
    11101 atagatataa aaaacaggaa gcagaaagga atttaagtaa tacaaaggaa aatttagaaa
    11161 gaatagatga tgtatatata gaaattgaaa atcaattaaa gcctcttttt aatcaacaga
    11221 caaaggcaaa aaagtacttg gaaataagtg aaaaattaaa gacacttgaa gtaaatagct
    11281 ttataagaga aattgaagga atagaaaaag aattaagtga ggttaatgaa catcgtaagg
    11341 ttattgaaaa ggagctcaat gaaaaagaag aacaaaaaaa tgttgtagag aaaaagcaag
    11401 aagatattaa taaagaggta gaggtattac aagatgtaat tgaaaaatct gtagattata
    11461 taaattcaat taaaggagtt atatcaaaaa aagaatccca aataaattta attaaggaaa
    11521 gaataagaaa ttttacgaat gaaattagta gaaaaaattt agaaataaag gatataaaag
    11581 agaagctaaa tgaaaataag caatacataa aagaattaga aagcaataaa ttatcaggta
    11641 gtgaagaact atctaccttg caggaaaata taaaagtatt ggaaggaagt aaagataaac
    11701 aaaaaattaa gttagaatcc ctaaataat
//
LOCUS       DAEMGG010000122        11653 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEMGG010000122 DAEMGG010000000
VERSION     DAEMGG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.123"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(204..461)
                     /locus_tag="KPK24_003064"
     CDS             complement(204..461)
                     /locus_tag="KPK24_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2300715.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            1041..1262
                     /locus_tag="KPK24_003065"
     CDS             1041..1262
                     /locus_tag="KPK24_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG2300716.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1953..2183
                     /locus_tag="KPK24_003066"
     CDS             1953..2183
                     /locus_tag="KPK24_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG2300717.1"
                     /translation="MEIKKKLVMSFKNVKDKPVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            2196..2345
                     /locus_tag="KPK24_003067"
     CDS             2196..2345
                     /locus_tag="KPK24_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG2300718.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            2602..3039
                     /locus_tag="KPK24_003068"
     CDS             2602..3039
                     /locus_tag="KPK24_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG2300719.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            3296..4189
                     /locus_tag="KPK24_003069"
     CDS             3296..4189
                     /locus_tag="KPK24_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2300720.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            4367..5191
                     /locus_tag="KPK24_003070"
     CDS             4367..5191
                     /locus_tag="KPK24_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2300721.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            5290..5898
                     /locus_tag="KPK24_003071"
     CDS             5290..5898
                     /locus_tag="KPK24_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2300722.1"
                     /translation="MGKFLRGKIMKSDSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            6002..6178
                     /locus_tag="KPK24_003072"
     CDS             6002..6178
                     /locus_tag="KPK24_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300723.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            7474..8226
                     /locus_tag="KPK24_003073"
     CDS             7474..8226
                     /locus_tag="KPK24_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300724.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKQLSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            8216..10327
                     /locus_tag="KPK24_003074"
     CDS             8216..10327
                     /locus_tag="KPK24_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300725.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCTVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISVVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            10711..11421
                     /locus_tag="KPK24_003075"
     CDS             10711..11421
                     /locus_tag="KPK24_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300726.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYKIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT         4478 a         1469 c         1462 g         4244 t
ORIGIN      
        1 aatattttcg accattttct ttgtattctc aatatatctc cagtatttaa caaatttttt
       61 tatagaatat acaatgtatt taaattaata agtagtaaat ttagttaatt tgtctaaaaa
      121 aactttataa aacatcttct ttgtctttac tcttacgttt aacaaggcta ttttcattat
      181 gtttaaacat tattttatta tatttaaaca ttattttcat tgagtattcg cttaatattg
      241 cttacacaca tattatatct aatagccaat tcattgtggt tgactccatt aaattgtttt
      301 actatatccc tatttctagc atatctaaaa agccccttat acgtaggtat ataagtattt
      361 gttcctccat acaacttggc aacctctata aacttatcaa ttccaattat ttctaccatt
      421 gcatgaaatt gaactggtat atcctccact tttatcttca taagttacac ctcctttaag
      481 atatttttat agacttaaca aagtttttat ttaatacatt attgactaaa ctatatttat
      541 tagttttatt gaatctaata aaaaatttct tagctttaaa attaattttt gtattaactt
      601 taataaaatt tctcataact tataactttg ttagctttaa taaagatttt aatggtttaa
      661 aagtaaaatg ttaatattgg acttttaact tttttattta cagattaaat ttaataaaat
      721 ttttaacata agctttaaca tttaatttaa cataaacttt agtacctaac atttaactct
      781 acacatatga aaatccatac aaaactatct caactaagca ggaatatata caaatactat
      841 ttcaaaacac taagaacata tcttcacaaa taacttgtaa actttttcat cagcaaaaaa
      901 gtaaaatttt tcctataaaa atttacacaa aaaatcttta gaaattactg tttaactccg
      961 atattatctt agatgatagt attatagcta acaaagaaag tcgacaaatc tttgaaatta
     1021 aatttttagg aggtaatttt atggaagttg tttcaacaaa aaatttatct agtataaaac
     1081 taaaattgga tgctggtttt gatgataagg gtaaggcagt tgttaaaagt aaatcatttg
     1141 caaatgtaaa agcagaggca ttaaatgaag atgtttatgc tgttgcagaa gcaatagcaa
     1201 gtcttcaaga gaaccctatt gtagatattt taaaattaga tagtacatca ttatctaaat
     1261 agtatctttt aaacgtactc tgctttacaa attaaaaaat taactcttta tataaaagat
     1321 taagttatat aacgtactta atacgctata gatttggcag attataactt caattttggt
     1381 tataacttta atatattaat gctttgaaaa attaatatat taaacgtatt tcaacttaaa
     1441 catgttagct attttagatg ctaccctact ttaatctaat taattgaaat aaattaaata
     1501 ttttctatat cagcaataac tagagtcaac aattaaaata ctaatattga caattgaaac
     1561 actgatacta acaagcaaaa cactaatatt gacaattgaa atactaatac taacaagcaa
     1621 aacactaata ttgacaattg aaatactaat actaacaagc aaaacattaa tattgacaat
     1681 tgaaatacta atactaacaa gcaaaacact aatattgaca attgaaatat taatactaac
     1741 aagcaagaca ctaatattga caattgaaat attaatacta acaaacaaaa cactaatatt
     1801 gacaattgaa atattaatac taacaagcaa aacattaata ttgacaattg aaatattaat
     1861 actaacaagc aaaacactaa tattgacaat taaaatacca atattaacaa gcaaacaatt
     1921 aaaatataaa ttattatatc ggaggtaatt atatggaaat taagaaaaaa ttagtcatga
     1981 gctttaaaaa tgtaaaggat aaaccagttt ctttttctat agaaaatcca aaggatgatt
     2041 taactgaaga aaatataaaa tctgtcatgg atttgatagt atctaaaaat attttttcgg
     2101 ttggaggatt tgacctagct tctgttgttg aagccaaggt tgtggaaaca aataccactc
     2161 cttatgactt agttatagaa tagggggcta attttatgga tgatagttta caactgttaa
     2221 taaccaatgt tggttttcct atagctctat ccatgtactt attggttaga attgagggta
     2281 aattaaatac tttatcaaac agtattgata cattatccaa aaatattttg atatttaaga
     2341 agtaatttga caatatatgt aagtcataaa ataggtctat ttgtaaaata attagacata
     2401 ttctatgtga aatactagga tatttggata atttatccaa atatccatag tatttttttg
     2461 aattttacca cctcaaattt gaatttcact gttttatttt tgaatttcac tatgcttaat
     2521 ttgaaattta gaacgcatct agttggactc aataagatat aatgctattc taatgacagt
     2581 aataaaatga ggtgatgatt aatgaaaata catgtcgaac aaggtattaa ttgtgatgat
     2641 attgaaataa ccataaaatg ttctgctcta gataataaca ttgaaaaaat aatttcatta
     2701 ttgagtacac cgttacttga tataaaaggt aaaaaatcag gagagattta taaactcaat
     2761 gtctgtgata ttttatactt tgagacagtt ggaaataaaa catttgctta ttatgaagat
     2821 gatgtatttg aaattgattt aaaaatatat aaattatcaa aattattagc taatacatcc
     2881 ttctcacgaa tatctaaatc catgatttta aatatggact acataaaaag tataaaagta
     2941 atatcacata atcgcatgca agctacttta aaaaacaacg aaaaagttat tataaatcgt
     3001 cgttatataa aaaatattaa agaaaagcta aacatttaat aaaaatctaa agtaatttat
     3061 taatatcact aattgcaaat ttattgacct atttaataat taataataat ataatatatt
     3121 caatattaaa tactaattaa ttatatttag tgtaaaaata attaatcaaa atataaaaaa
     3181 taggatatta ttataggcaa taactaacat caagcgataa cttaatacat ataaaaataa
     3241 ataaacaatt aataaaaatt aaaacttcat tataaaacgg aaggatagtg aaaaaatgca
     3301 actatataac acccccttta atacaaacaa acacttaatc tatttcttta catttacgct
     3361 agtattgaac tggatattca gtcttacacc tgcgttactc ggaattacaa atacaatact
     3421 tggagaaatc attttttatc ttgcatatac atcaccagct attgtaggtc tattttttat
     3481 ttctacaatc tatccacaaa gtgcaaaaca agattttatt tatagatacc taagctttaa
     3541 agaaatcgga ataaaatgga tactattaac aatattattt tttgctttaa cttttattac
     3601 ttcgttaata atcggaaatt actttaatgt aaaaacttct gatatagatt ggatacatat
     3661 agcagtgttt agaccttcta aaatagctta tatgctatta gcaagtttta tattgagtat
     3721 acttagtcaa gaagctggat ggagaggata cgccattgac aaattattag ttagatttgg
     3781 atttataggt tcttcaatta ttttagggct tgtttgtgga atctggtatc tagggtcgta
     3841 ttttacacct aatcaaatac catataattt ggcacaatac tccctatttg atgctttttt
     3901 atttatacca aacattgtac ttttaaactt tataattaat tttgtatata tcaatacaag
     3961 tagaagcatt ttatcagctg gattagttca tatgatgtac tattttttca acattcaatt
     4021 acttctacac taccctatta aattatgtgt aatagctcaa tatgtacaaa taatatttgg
     4081 tttaatattt cttgtatata tgatttcttc taataaattt aaacagaagg taaattctga
     4141 aattgaacag ataaagtcgg atgattttga atttgagtta gactggtaat tttaatatga
     4201 attataaatt aatataatct atgatttgat acgattacta cttaaatata atttactctc
     4261 tataatttat tgcaacatct ggaaaataat tgtatttcca caaacgcttg aagtatataa
     4321 aaacaaaata tatactgatt ataagtgaaa aggaggtcat attttcatgt ttaaaatagg
     4381 agaggtttca aaactaacac aaatttctat tcgtatgttg cgttattatg atgaacttgg
     4441 tattttaaag cctgcagaaa tagacaaata tacaggtcat agattatact cagttgaaca
     4501 gatttcaata ttacaaagaa ttgtattatt gagagattca aaatttagcg ttgcagaaat
     4561 agcaaatatc gtacataatt ggaatgatga atttgtaatt aaggaattga atagaaaaaa
     4621 gaatgaaata caaaaggaaa taaagcagga acaacaaaga ataaataaaa ttgataaatt
     4681 tatagaagct ataaactgtg ataaagatga aattcattac aatgttgtct ttaaaaagat
     4741 tccaagttat aaaatcatat cacttagaga gataattcca gactaccaat ctgaaggtat
     4801 actctgggaa aagctatcta aatttatcaa agaagaacat atagaagtat cacgtcaatc
     4861 caacaataac atagcttttt accatgatga agaagtcaaa gataatggtg tagatattga
     4921 agttggaatg gttgtaaaga agattggaaa gaacaaatct ggatttatat atcgtgagac
     4981 agaagaaatt gatatgatgg catgtactat ggtttatggt ccatatgaaa atatagctgg
     5041 tgcatatgaa tcgttctgtt attggttaga taaaaatagt gattatcaaa tatcaggtat
     5101 aaatagacaa atcggtcata aaggtgaaca taatgaaata aatcctgaaa attatcttac
     5161 agaaattcaa atccccctta taaaagtcta atcgattagt acttaaatac ttaagtacta
     5221 atcacccatt gacatcccaa aattttcatg atagtatcta aacgtgtatt aaacatgttt
     5281 aattttttta tggggaaatt tttaagggga aaaattatga aatcagattc aacaaaggaa
     5341 caattaatta atgctacaat ccagttacta tcttcacata gagatgtttc aagcattacg
     5401 gctagagaaa ttgtagctaa agcaaatgtc aatttagcta tgattaatta ttattttaaa
     5461 tctaaagagg agttaattaa tatttctatt aaaaaatttt taaagcgtca tatggatgaa
     5521 tataaggcta cacatgatac taaaaattta gcacctaaac agcaattaag ggaaatacta
     5581 attgcatttt ttgattttat aggtgaacat agtcaattta taaagatttc tgtaccctat
     5641 atattgatac aggaagaaat tgtttatcca tttgagatac tacctttaat taaggctcac
     5701 tatgaaaatc agaataacaa aaaaactgaa gctgaataca gaactattgc gtatcaaata
     5761 acttcattta ttcagttgct ttttcttcgt tctaatgctt ttttcaagta tagtggtatt
     5821 gatattctta atgttgaaca gagaaatggt ttaatagatt ttcaattaga tttattattg
     5881 gtagatatta caaaataata ctatattaat tttttatgta cattttttta ttttatggta
     5941 acgtttgaaa atattctcat tgactaaaaa tacttgcatt aaataagggg gaggaatttt
     6001 aatgtttttt acatttatag gagtaatatc agcaatttta ttgttgtgta caattatatg
     6061 tggtttatgg ataaaactta ataatcgtaa taatgactct aattctcact ttactttttc
     6121 aatactttca attgcttttt cactgttaat tgtggtggcc tttttagtaa gaaattaatg
     6181 tctttgttat gctggggttt attattatag atttttatta ttgcatgatt tttactatat
     6241 tatagtattt ttattatatt acagggtctt atttattata gtgttttgtt gttacagcat
     6301 tttattatat tacagagtcc tatttattat aggattttta ttatattgta aaatcttatt
     6361 tattatagga ttttgttata ttacaatctc ttatttattg cagtgttttg ttattacagt
     6421 atttctatta tattgcaaac tcttatttat tatggtattt tgttattgca acatttctat
     6481 tatattgtaa agtcatattt attacagttc tttattatac tacagacttt tattattaca
     6541 atctcttatt tattacagtt cttttctata ctacagacct ttattattac agcatttcta
     6601 ttacattgca aagtcttatt tattacagtt ctttattata ctacagactt ttattattac
     6661 aatctcttat ttattacagt tctttattac actacagact tttattatta caatctctta
     6721 tttattacag ttctttgcta tactacagac ctttattatt acagcatttc tattacattg
     6781 taaagtctta tttattacag ttctttatta tactacagac ttttattatt acaacatttt
     6841 attattttaa cttctttact ataatatagt aatattttaa tatctcaaaa tcatatcaat
     6901 cacttttcag catagtctaa aatacttcca ctttctaaaa ttatctatca tacctatact
     6961 aataatactt tttaaccttt ttattataca tttcaattat ttttaacaac ctatttattt
     7021 ccaaaactcc aatatatttt ctcaattaca ttgtgttaat agcccctaaa tcctttcaaa
     7081 ttatttacaa tacattctat taaataatct tactgctcta taattctttt tttcttttta
     7141 accctactat acattattta catcattttt ttcaatacaa cattcgttat tctataacct
     7201 ttttactttt actatatact tcttttctaa ctacaccttc aatgttccac atcatattac
     7261 tttagcacaa caattaatac ctaaaaataa ccctctaaat cctccaataa tataatcaat
     7321 ttaactataa taataacctc tcacactatc caaacaatat attttcaaac attacaaaaa
     7381 aacatccaac tattacaata tagctcatat ccctcctccc ctttcaaatg ctatcattga
     7441 cttacaagat aaatgaaagt gaggatgata tatatgctta aagtaaacca attatgtaaa
     7501 tcatataaaa caggcagtaa aacatatcct gtattaaaaa atatttcttt tgaaatcaac
     7561 aaaggagaat ttgtcgcaat catgggttca tcaggaagtg gaaaaactac tcttttaaat
     7621 tgtatttcat gttttatccc aattgataat ggggaaataa ttcttggaaa taataatata
     7681 agaaatttaa aagaaaagca actttcagaa gttagaaacc aacaactagg atttgtattc
     7741 caagatttca tgctatttga tggtcttagt gtatttgaaa atatatgtgt tccacaaatt
     7801 atcaaaggta gtcaagtctt acaaatggaa aaccgtgcaa ttaatctatg tgagctattt
     7861 ggtattgaac atatcagaaa aaaataccct gccgaaatat ctggtggaga aaaacagcgt
     7921 acagcagtag caagagcact aatgaacaac ccagatttaa tattagcaga tgaaccaact
     7981 ggtaaccttg atagtaagtc ttgtcgtaca gtcattgaat catttttaaa agcaaagcaa
     8041 gaattagatg ctactatact catggttaca cacgatagct ttgcagcaag cttctgtgac
     8101 cgagttattg tgcttaaaga tggaataatt tataaagaag ttgtaaaatc aaattcaaga
     8161 cataaattcc tagatgaatt actagaagtt ttaaaagaaa tgggaggcgc tcaagatgac
     8221 aattaataaa ttatacagta aacttcgagc ctataattgg aagaactata caactttatt
     8281 cttttgtata atactttctg tggtattagt aacttcatat gctctaattt attttagccc
     8341 aacaatacaa aacatacttc caactggtgg agatagcaga aaacaggcta caatgatatt
     8401 ctcagttgct atattaggat gcactgtctt tactacatac gcatcttccc tctttttaaa
     8461 atttaaaagc aaggaaattg gtattttcat ggctctagga acagataaga agaaattaaa
     8521 aaaacttcta ttttcagaga tattgttaat ttcagtagta tgctgttttt taggtcttat
     8581 attaagtatt cctgtatctt ttggtatatg gaaaatattt caattagtga ttattgatac
     8641 cagagaaatg acatatcatt ttggaactca aggattcata tatggattta tatttagtgt
     8701 atttgtgaca ttatgcatat ttatactttc tgtaaaattt attaatcgta caaatataat
     8761 tgatatatta aatgaccaga gaatatctga accaatacag gatgtaaagg actggtatgg
     8821 tatattaggt ataattttta ttatactcgg tatttttcta ggatatctcg taccttcact
     8881 aactataaac aaattaaatt ataaactacc atctatatgg agctttactt atctactctc
     8941 tcttattgga atatatatga ttatgacata catagttgta catgctaaaa gaggaaaaca
     9001 tccagagaaa tactataaaa acatcatatc aactagcatg atgagattta tgggaagaca
     9061 aacagtaaaa aacatgtgtg ttatttcatt tttgattgca ggagcattat ttgctgtatt
     9121 ctatgttcct aatatggtta caggtatcta tgatactctt aaaaataacc ctatagatta
     9181 tacttttacc tacaatcaaa gagataaaca ggtatcaaaa aaagatattg aacaattagc
     9241 agataaacac aatataaaaa ttcaaaacta tatggaaatt ccttctatag aattaattgt
     9301 tgatggtgta gaatcaatat atcataaaga tggaactatc gaaaaggtat atatgaaaaa
     9361 aagctcctat gatatgttct ttaattctaa agatttttcc aagctaattg gcaaaccaat
     9421 aactataaaa aatggtgaat atcttgctgt tgtaaataaa ggaaatgaaa atcaagatta
     9481 taaatatacc aaatttatat caagcccagt cacagaaaaa gaaatcccct ttagatatgc
     9541 tggaaaaata atatttaatg gttctttttt tcaaaataga agtatgaacg cctatgtcct
     9601 taatgaggag gattataaat catttactaa agatttatcc ataaaaaatt acaataattc
     9661 tatactattt aatgtagata aagataccta tgcttttgcc aatgacttaa agaaagaaat
     9721 tataaaaaga acatctaagg aaatgggcat gttcagtggt tatgatgaat acgtaaaaaa
     9781 acgttatgaa tcaactggaa gaaaatattt tatggatgaa gaatatccaa gtggtgaagg
     9841 tcatttaaaa ctagacccaa acaacaatca gttgtatctt aattggaaat actatcctaa
     9901 ctttaaaatt ttacaaagcc aagatatgat aaaaaacatg gcagtcttct taatgttatt
     9961 catatatgtg gcaatcatat gttttacagc agtaggaatc attgcatata caaggggtgt
    10021 tactatagct ataaatcaca agcaggtctt tatggattta aatcgattag gagcaaacaa
    10081 tgattatata aagttctgta ttagaaatca attaaagaaa gtattttcat accctcttat
    10141 tgcaggaagt tttttaattt atctgtttaa ttttatgatt atgttaaata atgatggtag
    10201 aatcgtcaat agtgaggtca atgctctttt gataaactta ggttttatag ggatttgtgg
    10261 ggtttatatg taccttatat atctattaac ttacaagaaa ttcaaaaaaa ttattggtat
    10321 tttataataa ggaaggttgt tagattttta aagtaataaa atcattggtg ttttataata
    10381 aaaaaggtta ttaggttttt aaagtaataa aatcattggt gttttataat aaaaaaggtt
    10441 attagatttt taaagtaata aaatcattac tttttataat aataaaagca tttgtatttt
    10501 ataataagaa agattgttag attttttaat aagaaaatca ttagcacttc agaataagtt
    10561 taatagaata gttttaagtg taagtatact aatataaaaa attttatttt gctatctgga
    10621 tataattgtt atttcatttt atatatctag gtagcaaaat atattatata cttaactata
    10681 taaatataaa atttgaaagg agataaaaaa ttgacttcga tttgtatttg tgatgatgaa
    10741 aaacaacaaa gaaacatgct gaataaaatc attacaagag aaatggattt gtggggctac
    10801 ccctataaaa taaaagaata ttcaaatgga gaaagtctag tatacaatct agaacacgaa
    10861 aattattaca tagatattat ttttttagat attgaacttg gaaaaagtaa tggcgttgac
    10921 atagcaaaaa ctatacgaaa acattcaaaa gatactataa tcatctttgt cacaggtttt
    10981 tctgactatg tatttcatgg atatgatgta ggagctttga actatatctt aaaaccttat
    11041 aaaactgaaa aaatatgtaa agtattaaaa gaagcactaa aaaaattaga acaatggaaa
    11101 gaaaacttta ttacagttca gattggaact aatctttgta aaattaatac aaaagatatc
    11161 ctctacttta aaagtgagct aagaaaatta actgtagtta cctttgataa aacattagaa
    11221 tactacggaa aattaaatga tatggaaact gttcttccct ctaattttat acgtacgcat
    11281 caaaggtata ttgtcaatct aaaccatgta gatagtataa ctcaaactta tgccaaagtt
    11341 cagaatgaaa aaattcctat aagccgtaaa aaatatcaag aggttgtagg aaaatttaca
    11401 aggtatatgt tagaatctta atttttgaag ttcaatctct agaattttat ttttgaaact
    11461 taacttttta aagcttagtt tttagagttt tgttttttca aatttaattt tttgaagctt
    11521 aatttctaga atttcaatct ttcaaaattt aattttttga agcttagttt ttagagtttt
    11581 tttcaaactt aattttttaa agctcagttt ctagaatttc aatctttcaa aatttaattt
    11641 tttgaagctt agt
//
LOCUS       DAEMGG010000123        11623 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEMGG010000123 DAEMGG010000000
VERSION     DAEMGG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.124"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(601..1317)
                     /locus_tag="KPK24_003076"
     CDS             complement(601..1317)
                     /locus_tag="KPK24_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300727.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(1856..2656)
                     /locus_tag="KPK24_003077"
     CDS             complement(1856..2656)
                     /locus_tag="KPK24_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG2300728.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(2970..3701)
                     /locus_tag="KPK24_003078"
     CDS             complement(2970..3701)
                     /locus_tag="KPK24_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300729.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(3688..4383)
                     /locus_tag="KPK24_003079"
     CDS             complement(3688..4383)
                     /locus_tag="KPK24_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2300730.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(4385..5176)
                     /locus_tag="KPK24_003080"
     CDS             complement(4385..5176)
                     /locus_tag="KPK24_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300731.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            complement(5186..5998)
                     /gene="modA"
                     /locus_tag="KPK24_003081"
     CDS             complement(5186..5998)
                     /gene="modA"
                     /locus_tag="KPK24_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2300732.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            complement(6633..6833)
                     /locus_tag="KPK24_003082"
     CDS             complement(6633..6833)
                     /locus_tag="KPK24_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2300733.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(7058..7234)
                     /locus_tag="KPK24_003083"
     CDS             complement(7058..7234)
                     /locus_tag="KPK24_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300734.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(7288..7377)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(7557..7757)
                     /locus_tag="KPK24_003084"
     CDS             complement(7557..7757)
                     /locus_tag="KPK24_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2300735.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(7759..8109)
                     /locus_tag="KPK24_003085"
     CDS             complement(7759..8109)
                     /locus_tag="KPK24_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG2300736.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     SKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            8271..8460
                     /locus_tag="KPK24_003086"
                     /pseudo
     CDS             8271..8460
                     /locus_tag="KPK24_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8937..9107)
                     /locus_tag="KPK24_003087"
     CDS             complement(8937..9107)
                     /locus_tag="KPK24_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300737.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESGYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(9496..9990)
                     /locus_tag="KPK24_003088"
     CDS             complement(9496..9990)
                     /locus_tag="KPK24_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2300738.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKFLND
                     KSNT"
     gene            10795..11172
                     /locus_tag="KPK24_003089"
     CDS             10795..11172
                     /locus_tag="KPK24_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2300739.1"
                     /translation="MKEEILIEKLLRVELIVMEYLWKKDTLMPKKKIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIINFQSYYKIIIDNKEYYTFKKKVLKSSKSIKIMHSLT
                     TIHKNVSKEKLDLLDEYYRNLKE"
     gene            11277..11623
                     /locus_tag="KPK24_003090"
                     /pseudo
     CDS             11277..11623
                     /locus_tag="KPK24_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         4348 a         1502 c         1364 g         4409 t
ORIGIN      
        1 tattggataa tagcttgtac aactatagat attaaaaata ctatagctaa ttttttattt
       61 atagtttttt tattatacat actaagtaag tttgaaaaga atacatatgt tatagacata
      121 tcttttagat tatagttatt atatttaata ttttcatata ttatatagac tatagatata
      181 attataaaat tgtagattat taaattagtg ttgtaaaaca ttttattact tgtaaatgca
      241 tgaaacctta aggttaatag tatcaaagtt aatataagtg taagttctat ttttacctta
      301 gaaaaaaggt ttatgacatt ctctaagaat ttgactttag tttgtttatt ttttttatac
      361 aatgtaaaac atagtatagt caataaaaaa cttgataata taatacctat gcagacaaac
      421 tctaattcta cttttgacaa atgtacagga gtaccgaaaa aataaaaata agggagagca
      481 aactctctat aataataatt aatattagaa aagttattat tcaaatcata aactgcttca
      541 aataatatag aaactaatag aaacagtaat aataataaaa taaattttgc tttagaatca
      601 ttaataatta agtttttcaa ttttgtaacc aatgccccac accaccttta aatattttgg
      661 ttcttttgga ttaatttcaa tcttttctct aattcttcta atatgaacag caatagttct
      721 ctcacaacta taagcatcct cattccagac attttcataa atctcatgag aagagaaaat
      781 tttacctttt cgtgacataa gaagtgtaag tattttatat tcaagtggtg tcattttaat
      841 ttcctcacca tctaaagaaa ctatttttgt atctgtattt agctctaggc caccactttt
      901 tagaatataa ttagtatcat ttgagttatt tgtattacta aaatcaccta aatttatata
      961 ccttctaagt tgagatttta ctcgagcagt aagttctagt ggattaaatg gtttagttat
     1021 atagtcatca gcacccatat taagacctat aattttgtcc atatcttctg attttgcaga
     1081 taagagaatt attggtatat tccttttttc tcttatttta agtgtagttt taagcccatc
     1141 taaattgggc atcattacat ccattattat taatttaata tcattttcga ttagtatgtc
     1201 caatgcttca agtccatcat atgcctttaa aacattaaag ttatcaggtc tcaaatagaa
     1261 ctctatagca tcaactatct ctctatcatc atctacaacc aatatatttt gattcaattt
     1321 attaaccccc ttgaaatttt atttttaacc aatttttatt ttataccaaa aagaggtatt
     1381 agaaaacagt aagcccgttc attatgaaat aaaaactagt acttttaata tattgtcaat
     1441 gcatatagta aatttaaaag tatagacgcc catattaatc tgaaggtaaa agtttatgaa
     1501 aatatctagg gtaatctatc acacttctta tgttattttg gtttattaat tttttaggtt
     1561 taaacatata ttaacaaata taaataacaa atgagtttca taattattaa gaatttataa
     1621 caaatattta ttttaatgta gtttaatata aacaggtagt ttaatgtagg tatgtataaa
     1681 gcatttaata aggcttatag gttttaatga tgatatcttt atatatttta agagatgatg
     1741 aaattagata tgggtaatag ttgtatgaga aaaagtagta tagcacaaaa agtgctatat
     1801 cacaaaatct caaataattt cctttttata acttaaatta aagaataaat atttcttata
     1861 aattaggtgt atgaagtata gcaggaacat ctttattatt taataactta tcaaatgatt
     1921 tatggtcaga cttatagcca ataccttctc tatattctaa ttcatcaaca attttgtatt
     1981 gttgacaggc atttttaacc ttttcatagt cctcagtaga atgagcttta taaactatct
     2041 tattattaaa agttatttta tcagcaaatt taggttttac aaaatattta gcatctttta
     2101 ttttaccagc attatataaa ttttggataa cagaaccatt ttttaaatgt tgtaaatggt
     2161 catctaattt ataagtatgt gctatatgag ttacattttt aaatttttct tcaaatgtaa
     2221 gagcaatttt ttccatgtaa ttgaagtcag tattaccatt tgtatttgtt tctggtaata
     2281 atattatatt ttcccttatt acaccaaggc tattaagaga agctaaaaat tctctatttc
     2341 tatattctga tttttgtata tttgttaggt tttcatattt tttatattta tcaaagacgg
     2401 atataccaca tccagtagat actaaaacca caaatacatg attttttcct ctttctttta
     2461 tagcattaac aatagcactc ccagcccaca acacttcatc atcttgatgc tgaacataga
     2521 agacaacata atctttaaat tttttatgac tataattcac atagctacta aaagcggtta
     2581 aaatcaaaca taaaactaga ataatagata gaattttata tttatttata gtaaattttt
     2641 tattcttaaa tgtcatttaa tcatctcctg aaaggttgtc ttagttatat tatcaaacaa
     2701 agtactaaaa attatttctt aaatgctgaa aaatatagag tagattaatg gaataaaatt
     2761 tttttattag tttaaaataa agaaaacatt tatgtataag actaatcaaa atattaatga
     2821 ttgataggaa ttgagattta aagggattgt acatttattc gggattcatt ttaatagaat
     2881 tttactttag tacttagaat aaaagtaata actttttaaa tttaaagagt acacatatat
     2941 atttgtgtac tccttgatga tatgattgct tatttactac aaaaacagag tctttttaaa
     3001 cctaaaatat ttgatgcacc agctatcgta gttccataat agtaaactgg tttgttaagt
     3061 gatagaaaat ttataattga gttattacat aatgtacttc ctgtgcaaat cactaaatca
     3121 gcccaagata ttacatcttc ataatctcgt ataccatctt caatcttaac attatattga
     3181 atagtatcca caaattcagg atttaaatct aatactcttg tttcgaaaaa gtccttaata
     3241 ttatcaatta tggctggttg atatcctata attgctactt ttggattacc aaattccatt
     3301 tttataaact cagggaattt ttttgcacat acctctggct cgttattttt acaatgaatg
     3361 gttctatccg tcttgcctaa gtatttcata actgcattta atgatgctat aaaaagactt
     3421 ctatcatgag aattgtcatg taaatctaat gataatatgt cttttaaaga accttcaaat
     3481 gtagatggag tagaagtaaa tgcttgtcct ttagctcctt taaagtcagc ttcaagcatt
     3541 atttctttac caactataat tgggaaatct ttacgcttag tatttccaat tgcttctttt
     3601 gaagatagag atttggacac aatacttatc atatccatat ttaaattata tttatcgact
     3661 tgtactaata gataatctct caaaatatca aatatattca tagaaaaaag attctccttt
     3721 attttttatt tcttcattta aatcaaaaat atgatttatt tttccattgt tcattattcc
     3781 tatcttatga gcaaaaaaca ttgcttcaga aatatcatga gtaacatgaa tcactgttgg
     3841 tttgtatttt atgtagacat ccttcaaaat tttgcttata tgtttttttg taatataatc
     3901 caatgaactc ataggttcat ctaaaagtaa tatatcaggt tcagctatta aggcacgtgc
     3961 aaaagcaact cgttgctttt cgcctccact taaattttta ggatacctat tttttatatc
     4021 ttctatctgc attaatttag acaagtggtt agccttaaga atctgttcat ctataggtat
     4081 ttttctcaat ttaagtggaa accttatatt atcttcaaca ttcatatttg gaaataattc
     4141 aaaattttga tatacaaatc ctatatttct attttctgga ggtaagtttg atattaaatt
     4201 ttctttgtat gatacatttc ctttagcaga tggatatctt cctgctaatg tttctagaaa
     4261 tagagtttta cctgacccac tagctcctaa taaaacaaag tattcatgat tattaatatt
     4321 taaactattt tcttgtaatt taaaattagg taaatcaaga gataaatttt ttatttttaa
     4381 catattaatt cttatcaacc tctctattaa aaaatatctc aaaaagcatc aagcatgtaa
     4441 gtgagagaaa tatcaaaatt acagctattc cgatagcaat gtctatatca ccagtagaca
     4501 tatttaaata tatagatgta ggaataattt cagttttcat acgtgtagaa cctgctatca
     4561 tagctactgc accaaattca ccaagtgctc ttgaccaagt cattacagtt gcagatatga
     4621 ttccagtttt tagatttgga agaattataa atctaaatgc ttcccaacta ttacatccaa
     4681 gggtacgtgc aacaaatatc atcttttcat tactttcatt taatgaagtg tgaagtattt
     4741 taatagtaag tggaagattt acaaatattt gtgctaaaac tataccttgc tttgtaaata
     4801 taaaatcaag cttaaaaatc ttataaatgg aatctccaat ccccattctt ccaaacaaaa
     4861 gtaaaagagc aattcctgat actaagtgtg gaagagacat agggagattt attatctgta
     4921 taatcagttt tttaaatggt aatttagtaa tatgtaaaaa atatgctact ggaatagcta
     4981 ataaaaggca tactatagta gatattaaag atgttttaat actaagtgtt attgcatact
     5041 gtacttccac tgattttaag catataggaa cataacttat ccctttaatg attaacataa
     5101 gaagtggtga tacaataaac aaaaagacta tagcaaaaaa tccagtaagt agagtagtaa
     5161 aaaatttatt tttcaagttt aatcactact taacaggttc aaaaccatat tttttaaata
     5221 tctgtttagc ttcgtctgac tctatataat ttataaattt attagctaaa tcttttttat
     5281 cactatagtt tacgattcct gctgatatga gttggtctat atttttatct tgggcaattt
     5341 ctataacttc tacatcttca ttaccaaaga ccgaatcttt tgtggctatt gtagcatcag
     5401 ctttacttga tgttaactgt acaatcattt catttacagt tgcagctgta gagacaacat
     5461 tatcatttat agattttaaa ttattttttt caagtatttt ttgtgtagtc ttacctatag
     5521 cattagactc tttgtctccc aaaataagtt taactccatc tttatttaag tcttccaatt
     5581 tttgaatatt agctggattt cctttaggta caactattgc aggtgtatga tgtgcaaccg
     5641 ttttatgttc acctacaagt tctttttcaa aggcctttga ataaattggt tcagaaccaa
     5701 caataaatac gtctcctttg tgacttgtct caatttgagc tattagctgt gcagaacctg
     5761 catagctata ttcaactttg acattatttt ctttctcaaa tttttttgct atttcatcca
     5821 ttggtttctt taaacctgcc cctgaataga caaaaagagt ctctttttct tttgaatcat
     5881 cagctgttgt cactttagta ttagattcat cattagcttt gttttcttta gaattacatg
     5941 cagtaatact taatgaaata atcatagcta aagctagtac tgaaagtttt ttaaacataa
     6001 gatattcccc cttattaaat tagtgtaaat tcttttttag tatatcatac taagaaaacg
     6061 atgtctaagt ttttgttatg ttttataatg ttttgttatg ttttgttatg tatagttaag
     6121 aaaaaatcta ttcataatgt catttccata ggtagttatt ttatttttaa tttttattaa
     6181 tactttaaag tattttaaat ttaaaaactt aaatcaaatt aacttattgt agatttttta
     6241 ctttttaact aattttatat ttattatatt tcagtgaaaa aatagaacat tgaaacatat
     6301 agatatgttt taattgcttt ttattaacaa attactaaaa gtattatatc agaattgaat
     6361 acaaaagaaa gagtatgtat ttatgagata gttgaaaatt ttgatgtgcc acaactaaaa
     6421 ttattatatt actatatatt ctaattaaaa gagtacttgt aggtttaagt acatacttta
     6481 taaatatatt tttgtcagat ttattttata aaaagtattt agactaagta ttatttttaa
     6541 gtattttttt gttgaagtta ttttttcctt ttttacaaaa tgcgtaaata aaaaaaagac
     6601 tcttaaaaga gtctttccga aaaatctata tattataata tagttatatt ttctgcttga
     6661 ggacctctag cgcctttaac tatatcaaag cttacttttt gaccttcttc taatgattta
     6721 aatcctgaag tttgtatagc tgagaaatga gcaaatacgt catctccact ttccattgat
     6781 ataaatccaa atcctttttc attattaaac cattttacta ttccattatt cattaaaaat
     6841 actcctatta acctatatca agatatactt gatttacttg tgtattacca caaatactat
     6901 tgtatcatag attaacataa atgtctattt aattataatt tttagttaac atttttattt
     6961 attcttaata tgaaaataaa tttaaacatt ttatattacc aatatattaa tgtaaatgta
     7021 gataaaacta aaaagggttt tctagaaaaa aatctgtcta attatactta cttaccaata
     7081 ccatcacttg atgcaagcaa gattccacaa ctttttctcc gtactctaat ataaaatcat
     7141 ctaactgtgt atctataaaa tcacaataat catatgaact accaatataa gcagttaaca
     7201 tatgtgtaca tttatccatt ttatctctaa acataatgta tttatccccc taaaaaactt
     7261 tcatgaaaaa caaaaagaaa atatcttttt ggcaactggc tgacataact tattttataa
     7321 gcgttagtcc ctatagcttt gcgtcactaa atttctctag ttttgccgat ttagttttat
     7381 tctacagatt aataatacaa tagaattagt tattattcaa catatctagc tgaaaaatta
     7441 ataaatttat atatgaacgt taaattatct gaaaaaataa aaagcccctc tgtcaggagt
     7501 gacttaaaaa ttgaatttaa gtttgtattt aaaacacatt tttaataatg caaaaactaa
     7561 tcaaaaataa acaattcatc aataggtgta tctactattt ttgatatatc tattgctaat
     7621 ttcaaagaag gattatactt tcctgcttct aaacgcataa tggtttctct tctaactcct
     7681 actttttctg ctaattgttc ttgagttaat tcttttaatt gtctatattt ttttaggtta
     7741 caagtgaatt taggcatatt atttttcctc taaaatataa agttttatag cataaacatt
     7801 taaagtgatt gcatatgtaa ttgatagtaa aacacacatt aattctatat tcctagacac
     7861 tatagtaata caccataaaa tgaataattc tataatagca agctgtcctg taaatgccat
     7921 agctgctttt tcatcttgta catacctttc atctgcttta tcaccattta tttttgatat
     7981 aataaagttt gagaaaaaag caaaaaatcc aatatatgta aggtcagcaa cattgccaga
     8041 tgaaaaatat ctaagactta aaaatccaag gaaaccacaa aaacccataa aaaatttttt
     8101 ctgaatcatt ttttatcctc cttagtaatt ttaaaagaga tatatttata tctttatgat
     8161 acaaatatat ctcttttata ttcttttgtc aatagaaaat cagtattata caatatttgt
     8221 actgcaccat aattgttaga gcaaaaatct actgcttgag atgtttatgt atgactaaat
     8281 atgattttaa tttcaaatta aaagtagcta aattttattt gaatagagaa ggaagagata
     8341 ttttaatagt aaaacaatat ggagtttata accattctca agttgaaaga tgggttaata
     8401 tattaggaga gtgtgcacta aaaataaaaa aaattttcta tactatatgg ttcaagttag
     8461 atgttataaa ctatatgatg ataactaaat atagtgtaca ataaatcgct aagtattttg
     8521 gaataaacaa tagcacagtt atagcaacat gaaaataaaa atttttacat agtggtgttg
     8581 atgccttttc taaaatgaaa ggaagaccat aatgaataaa aacaaaaaca ctaaagaaaa
     8641 aataaattaa caagagaaca agaattagaa tatgaaaata aatttttaaa agatgagctt
     8701 gagtttttaa aaaagatgtg tgcctcaggg aagccacatt aaaaaacagt ggtatcatta
     8761 ttaatcaaaa gaaagttaga tgtattatca aaaaattaga acttaaatgc atagcatttt
     8821 ttcataaatc acataaatat aactcctata aatgtataat tggaaaagta aaaaataata
     8881 aaacaaatag aagatttaaa atatcaatag catatcagaa aataacaaat gatatgctag
     8941 aatttataaa atatatctaa tgataaaaat ggaaaatcaa ttataaaaaa ggcatatccc
     9001 gattcatttt ttgttatata gtcaaataag aatactatag atgaaagata cgattttgca
     9061 tatgtattaa gtaagtattc tgatgttttt ggttgataca catgcaataa agaagttgtg
     9121 tttgcagata aattagcaaa actatagtat aagttgcaac aataaagaaa atgaaaatta
     9181 tcatactaaa gcataaggca tgttacaatt ttattagata ggacattaac atacattaaa
     9241 gtacttttta ttataaagag tacttatttt tttgaaattc attaatgtat aaactatcaa
     9301 gaatattact caacataatt taaaaacaac ttgaataggt atttttaaat tatgtgatat
     9361 aataaaaaat agaagtgtat gccaatacac ttctgtaatt ataactaagc attctattct
     9421 tgaggagtgc ttttttcatt tctccaaaca agattgttta tttcatcagc aattattaaa
     9481 taaaatttat tgtatttaag tattcgattt atcatttaaa aattttatta caatatctat
     9541 tttgttttgc tcgttcatta tgtctattga acttccataa tcacttttta atttatctag
     9601 accacttttg cttatttctc cttctggaag tatacttaat tctagatatt tttcatgacc
     9661 aggagatgac attaatagat atgaaccatt ataaggcata cctaaatttt ctattttatc
     9721 tgctaatggg tcatctttaa agccaccatt tattattatt tttccatctt tctttaccca
     9781 tgatatcatt cctccatcag tatattttga ttcatctgca tagtagctaa gagacgcact
     9841 ttttattgca cttatatcag cttcaagttt agctatcttt gctttttcta tatttttaaa
     9901 taatgctgga agtgctacta ttgccaatat tcctattata gcaattacta ctaataattc
     9961 cactagagta aatccttttt tatttttcat ttatttcccc tccatataaa attaataata
    10021 tataaatttt tattttgttg atttctactt tctatatttt accatatata gacaaaaaaa
    10081 tcactttcta tgacaaatat actattattt tacaatttat ggaaatgatt taaaatgaca
    10141 tttcttgtta gaaaagatat aagaaatgga ttaaaataaa gaaaactaca gtcatatcaa
    10201 tgtcaataat attatttaaa agcagatgaa aataaaaaga ggactcagaa aataatgtct
    10261 atttaattat acttacttac caataccata acttgatata aacaagatta tactacattt
    10321 tttttatgct ctagtataaa atcatctaac tgtgtatcta taaaatcaaa ataattatat
    10381 gaactatcag tataataagt caaaatatgt atacatgaat acatatttta aaagatatat
    10441 attattacat acctaaaaaa ctttcataaa aacaaaaata aaatatgctt ttagtaactt
    10501 gttaacataa cttataattt attttatagg cattagccta tatagctttg tgctactaaa
    10561 tttctttgat tctagcgatt tagtgctatt ctacaactaa aataatacaa tagagctatt
    10621 tattgtttgg tagcattgtt tgcaaattaa acaaatttat atatatgaat attattttac
    10681 gtttaaaaaa acagactaaa tatgtagaat atttggtaaa acttacctct agtttttgtc
    10741 ttaaaaagct cctattatta atttaagtta gaagaattaa ggaagtgaca taaattgaaa
    10801 gaagaaatat taatagaaaa attattaaga gtagaattaa tagtaatgga gtatttatgg
    10861 aaaaaagaca ctctaatgcc taaaaaaaaa attgtaaaag aaataaaaca gatttatgga
    10921 tggcataaaa gtacaataaa aatactacta aaaaggctag ttgataaaag gtatttggct
    10981 agatatatta taaattttca atcttattat aaaataataa tagataataa agaatattat
    11041 acttttaaga aaaaggtact aaaatctagt aaaagcataa aaataatgca ttcacttacc
    11101 actatacata aaaatgtaag taaagagaag ttagacctat tagatgagta ttatagaaac
    11161 ttaaaagaat gatatagtaa aaatcgaatg taattattta ccttaattcc tttgtttgta
    11221 aaactttatt gatatagaat ttgacatata cataaattgg taaagggatg tatactatga
    11281 aaattagtaa attaccagaa gcggaattaa aagttatgag atatatttgg gaatcagaaa
    11341 aggtattaat accaagagaa atcaccaagt ctatggaaca aaaatatgaa tggaaagata
    11401 caactatatt tacagtatta aaaagattac taaagaagag agtttttaga agcggagaaa
    11461 atagataagt gtacacacta taatgtatta gtaaaaaaaa ataatatcag agattttaaa
    11521 caaaagaatt tttaaagaat acacataaaa attcaatatt aagtttaatg tcagcactat
    11581 gtagtaagga tgatgaagta gatggtgata ttttaaattt att
//
LOCUS       DAEMGG010000124        11530 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEMGG010000124 DAEMGG010000000
VERSION     DAEMGG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.125"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            237..1151
                     /locus_tag="KPK24_003091"
     CDS             237..1151
                     /locus_tag="KPK24_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG2300740.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRYYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            1541..2188
                     /locus_tag="KPK24_003092"
     CDS             1541..2188
                     /locus_tag="KPK24_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG2300741.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(2355..2924)
                     /locus_tag="KPK24_003093"
     CDS             complement(2355..2924)
                     /locus_tag="KPK24_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2300742.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(2929..3828)
                     /locus_tag="KPK24_003094"
     CDS             complement(2929..3828)
                     /locus_tag="KPK24_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2300743.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGILLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(3839..3988)
                     /locus_tag="KPK24_003095"
     CDS             complement(3839..3988)
                     /locus_tag="KPK24_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300744.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(3981..4565)
                     /locus_tag="KPK24_003096"
     CDS             complement(3981..4565)
                     /locus_tag="KPK24_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG2300745.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            4954..6279
                     /gene="cdeA"
                     /locus_tag="KPK24_003097"
     CDS             4954..6279
                     /gene="cdeA"
                     /locus_tag="KPK24_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG2300746.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFISSLLVFIIALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(6374..7168)
                     /locus_tag="KPK24_003098"
     CDS             complement(6374..7168)
                     /locus_tag="KPK24_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300747.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(7169..8044)
                     /locus_tag="KPK24_003099"
     CDS             complement(7169..8044)
                     /locus_tag="KPK24_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300748.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(8048..8416)
                     /locus_tag="KPK24_003100"
     CDS             complement(8048..8416)
                     /locus_tag="KPK24_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2300749.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(8599..9258)
                     /gene="deoC"
                     /locus_tag="KPK24_003101"
     CDS             complement(8599..9258)
                     /gene="deoC"
                     /locus_tag="KPK24_003101"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG2300750.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(9294..10481)
                     /gene="murJ"
                     /locus_tag="KPK24_003102"
     CDS             complement(9294..10481)
                     /gene="murJ"
                     /locus_tag="KPK24_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG2300751.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(10558..>11530)
                     /locus_tag="KPK24_003103"
     CDS             complement(10558..>11530)
                     /locus_tag="KPK24_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2300752.1"
                     /translation="TFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFF
                     KEKAGFPKFKSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQGRILNA
                     TISKEPSGRYYVSLCCTDVDIEVFENTNNQIGLDLGIKEFCISSCGEFIENPKYFKKS
                     LNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLTTKLIKENDIIC
                     IEDLQVKNMIKNHKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKC
                     GYKNEEVKNLNIREWICPSCNETHDRDINASRNILKEGLRLITIQNK"
BASE COUNT         3992 a         1792 c         1300 g         4446 t
ORIGIN      
        1 tcataaaaat ttctagtatt acttgtactc ttaataaata atagccaaca agtaccttaa
       61 atcattatat ctatatctaa taagaaacac agtaagataa ttttttcgcc cataacttaa
      121 taaaaactgt tgatattagt agcaattgaa tttagttaat ttatttataa tatacataaa
      181 tttaaatatt acgaataaag tattataaat aggatacttt ctaaagggag gaaattatga
      241 tagataatca aaaatatgtt attttatcac tagaattaca tttatttttt tcaagaatta
      301 tgaaagagca tgctcttttt ttagaagcag gattcacaaa taaaaattat aatcttgcta
      361 tggaagctga ccactataaa aagcaatttg aagatttatt atcatacact gttagtgcta
      421 gtaatggtat aattagacct gatatattat attcagaaga gcttgtaact actctcacat
      481 cagttgcaga acaaaaaaca gaagagttta cagggataga aataaacaaa aacatcacta
      541 caagagaatt aaatctacaa agtggtgtaa acccacaagt tggtcaagac ttagtgaact
      601 atgtagctca acttaactct gatgcaataa gattacttga tgggcttatt aattttaaag
      661 aaagagtctt agatggtgta ctatcatgta ctatatttac atcaaactac cctctacttc
      721 ttgaacatat aatacatgaa gcaaatttat atcgttatta tgtagttgac cttgaaaata
      781 aaatagatat tgagtcaaaa aacgctaaag aaatagaatt attctgggac catattatga
      841 tggaacatgc tctgtttatg agaggattac tagacccctc agaaggtgaa ctaataaata
      901 cttcaaatga ttttgctata aaatttaatg aattaattga aaaaacaaac gaaatgactg
      961 attctaatat caagaacatt acagaagaaa ctctaaatga aactgttgag tttaaagatt
     1021 ttaaagaagc aggagcatca ggaatagaac agtgtaagat aaaatctata atattaccac
     1081 ttttagcaga ccatgtttta agagaggcaa atcattatat tagaatattg gagagttata
     1141 aaaacatgta attaaaaatt acttttaaaa taatctttgc atatttagat aaaataaagg
     1201 tacagttaac tgtaccttta ttttttataa acttataaaa tttaaacaag ttattcataa
     1261 aataaaataa taatcatcta taacccacaa ctttatttta tattaaataa aaagtattag
     1321 tgattatgtt ttataggtat ttctttattc agttatttat taaaaactaa tagtaaatct
     1381 tatactaatc gcatatttat gttataacta tattgtaact attttcgtaa tcttaggaaa
     1441 agattactta taaaattaac cttatacaat aatatatcgt ataatcttaa ttagcataat
     1501 agttacaata ttaaaattta aatatgggag tgattttagt atgataagac aaacaccatt
     1561 agagaaacga tatgacaagc taggtcctca tattgtaaca gcactcaaaa aacgttattt
     1621 tgatgcttat tactgtaaaa ctagaaatga agctttgcaa caaattttag atttaattcc
     1681 tcaaaatcat gtagtatcat ggggaggatc tgaaacactt aaagaaatgg gcgtacaaac
     1741 tgctgttaga gagaaaggat ttaaagttat tgatagagat ttagcaaaaa ctccagaaga
     1801 aagaactgaa attatgcgtc aagcacttct atgcgatact tttttgatga gtagcaatgc
     1861 aattagtgaa gatggacaac tctttaatat tgatggaaat ggcaatcgtg tagctgcaat
     1921 gatatttggt ccaaaaaatg ttatagttgt agctggtatg aataaaattg taaaaaacat
     1981 tgatgatgca atacaaagaa ctagaacaat tgcatctcca gcagttgttc agcgttttca
     2041 agatgtcaat acaccatgct atattaatgg aagttgtata gattgcacaa gccctgaaag
     2101 catttgtgca tacatggtta ctacaagaat cagtcgtcca gcaaaaaaaa taaaggtaat
     2161 attggtagga gaaaatcttg gtctataaaa tatacataca tgtaaatagc tctataaata
     2221 ataaaatcat atgaaataac ttatttctaa aagtggtaga ataaaagctt ctacaagact
     2281 ttcattctac cacttttata ttcttttaat ttgtgtttct atattattaa ttgctccgat
     2341 tttcatatat attactaact atttttattt tcataatttt tttgttcttc atcaagctgt
     2401 ttataataat ctttattata gtcagctaga taatctatac cacctcctgt aatcacattt
     2461 aataatttca ttgcagattc ttgattaact ttacaaacat aattatcacc agatttaaca
     2521 agctctaatg taatattaaa ttcttttttc tttgcagaat caatatcttt catagttgga
     2581 tttggcatca tttctcctag tttaggagga ttctttttta aaatagaaac catatcagta
     2641 ttgttaattt ttaaatctac agtcacttta tccttattat tctttatatc tgaaatttcc
     2701 caagtcatat ttttagaata cattttaaat ccttctttat cttcgtatat aagattacct
     2761 aaccctgatt ttgggaaata cttatcaaca gtatctgcat cttgtgtttt tattgaatta
     2821 aacatactat ccacttcatt ttttatctta ttatcattag tacaaccagc taacaccagt
     2881 actgatattg caaacattat aatacatata ataaatttct tcattaaatt atatccccct
     2941 atactttttt gcacaaatat tattctcttt tttatctttt gaaaaagttg tagtaattgc
     3001 attttgtgga caaactttta tacattcacc acacctaata cagtctggac tatttacatc
     3061 tttatatatt ggtatatcaa gcttacattt tgtttgacat gctgtacaat ttgtacattt
     3121 atctttatca attttaaatc tatatattga aataggatta aaaaaaccat agattgctcc
     3181 taaagggcat atatacttgc aaaatggcct ataagtaatg attgacatca ataatatcaa
     3241 tagcaaaata cttaacttcc agctaaataa aaatcctatt gattctctta aattctcatt
     3301 tcctaaaata agtagtattc ctcccaaaag tgttcctgaa gggcaaatcc atttacaaaa
     3361 ccaaggattt ccaccccctg ctacatttac aatagcaact ggcaaaataa ttacaaacat
     3421 tattaagact atatacttta gatattttag atatttatca ccataaactt tttttatctt
     3481 tactggaaag ggtactttat gtagtaaatc ttgtacaagt ccaaagggac aaagccaacc
     3541 acaaacaaat ctgccaaaaa aagcaccaaa tgccattaaa aaaccaacaa cataaaatga
     3601 gaatttatac tctctgcttg ataaaactgc ttgtaatgaa cctattggac atgctccaag
     3661 tgcacctgga catgaataac aattcaatcc aggtacacat aattttttac tttctcctcc
     3721 atatatagtt ccttctaaaa atccatttac atatccattt gttaatgctg taaatgcaac
     3781 ttgagtaata agtcttatat tattatttac cttagataat aacttcatcc ctcttctcct
     3841 aaccaatacc aatacactca agacaaatca taattgcttt agttaatact gtatttgttt
     3901 ctccacgata tgtacctaat gcaataaata taatactaat tgcaataata gaaatgcctt
     3961 ttttattatc tttaataaag ttactcattg gcttttacca tttttagtct ttcatctata
     4021 atttttttcc aatcttcctt actatttgca cctacaacct tctcacccac aaagtttcct
     4081 ttcttgtcta caaaataagt tgttggaaac tccataactc ttttaaaatc agttttttta
     4141 agttcttcag atacagtcaa ttgttggtaa gttgcatctg actttttcat aatgtcttta
     4201 actaaatttt tctcttcatt tgaaagacca gttctcatat cagttttatc aacttctaca
     4261 actaatccct ttatagctac attggaatta ttactctcat attcttttga taatgcttca
     4321 agctctggca tttcacccac acatggtcca caccaagtag tccagacatt aataagagta
     4381 agttctttat ttgaaaatat ctcttttgtt accttatttc catcaacatc ctccgtatct
     4441 aaattactaa accattcact attattcttg tttttttcta ctggcttaga ttcttttttg
     4501 ctacatccag aaatcatacc taaaacacaa atacatccta aaactactat gaaaaacctt
     4561 ttcatatctc acactcctag aatttatata gtatataaaa tcgctgcttg aaataaaaat
     4621 aacttatctt ctatattaaa gtaattatat ttaataaata agagctaatt taaaaaaaga
     4681 tatacagtta aaatctattt cttttcagac aaaatcttat atatatataa tagcttgtaa
     4741 atcttaagat tatatacata tatttctaaa gattttctta agaaacagtt agattttact
     4801 aatataagtt tattaattag cattatacta atttatctta atatgatttt ttgttgattt
     4861 taagctgttt atcgtataaa taaaattttt cttgactata tagttactat atagcttatt
     4921 atatatagag agtaataaaa ggaggtaaaa tttatggaaa atttatttac aagaaaattc
     4981 actacttttg aatttctaaa atttgtttct cctgcaatta tatccatgat atttatatct
     5041 ttgtatacaa taatagatgg catctttgta tcgacattag ttggttctga tgctcttgct
     5101 agtataaata ttgtactacc tataattaac cttgtttgtg gatttggaat aatgatggca
     5161 actggtggag gtgctatcgt ttctatacgt atgggtgaaa atagacagga tgaagccaac
     5221 tctacatttt cttttatagt tttgttttca ttgattgttg ggattttatt cacagtaatc
     5281 tcatatttct ttatcaaaga aatatctatt ttgcttggtg caacagataa gttattacca
     5341 tattgtataa cttatggtaa agttatgatt ttatgtacac cattttatat tttaaaattt
     5401 atatttgagt actttgcaag aactgatgga aattctaaat ttagtttatt tctatcagtc
     5461 attggtggtg taacaaatat aatattggat tatgtattta ttaaatattt tggaatgggt
     5521 cttttaggag ctgcagttgc aactgctata ggtattattt taacttgtgt tttaggtatt
     5581 atttacttct tatctaataa atctacacta aaattaagaa aaccaaaaac cgattttaga
     5641 cttataagag atactatgat aaacggttct tctgaaatgg ttacagaatt atctacagga
     5701 attacaacat tcttatttaa tgtagtagct ttaaaattag caggagaaaa tggacttgct
     5761 gctcttacta tagtattgta tgctcatttt ttaatgacat cagtctatct aggattcgct
     5821 gctggagtgt ctccattaat aagctataat tttggtgctg aaaacagtga taaattaaaa
     5881 gaaacattta aacattctct aaaatttata tttatttctt ctcttttagt gtttattatt
     5941 gctttagtat ttgcaccatt tattgttagg gtctttgtaa atccagataa cacagtattt
     6001 aaactagcct tacaaggatt aaaaatattt gcatttgctt ttttgtttgt tggtataaat
     6061 atatttgcat caggattttt tacagcattt cacaatggaa aaatttcagc tattatatct
     6121 tttagtcgtg cctttgtttt tataatcata ggaatcataa ttcttcctcc tatgttaaac
     6181 atgactggat tatggcttac agttccattt gctgaggtta taaccatatt tatatctatt
     6241 ctatttataa aaaaatataa atgtagatat aagtattaaa aattaatact taaaatcaac
     6301 atttattaaa actaataaac ctgctttaaa atacaaattt acaaatttga tttttaagca
     6361 ggttttattt tagttataat aagtcatttg catactcttt aattggtgct tttctaagta
     6421 ataaaattat aaatattatg caaataacta taattcctac ctttgttcca aataaaataa
     6481 aaggattctg aaatgaataa ataatttttt ctaaatacaa tagcatttta tgcatatttt
     6541 tagttgcaaa attaactata taaattacca ttaatatcgg taatcctatc aatatagtaa
     6601 tactagtaac ttttctaact ctatatgtaa aagctccaag taacactcct ataaaaccta
     6661 tacatatttc atttatacat atttgtacaa aataatttaa ataagttagt ggcatacttg
     6721 atgttgaagg atctatcagt gctgatatag atgtccaaat catatctctc tttagtaaaa
     6781 ctgatagttc tatactgtaa cctgttataa tttctataaa taacttcgat aaaaaaatca
     6841 aaactatacc taaaatagaa atcacaaagc ttaaaataat taaatctatg acaatagctt
     6901 ttatacaact ttttctatct gctcttatac ttaaagctcc tgaaaagtct ttatttgcta
     6961 tatttactcc atacacaaat agcagtatag ttatatatgt taaaatagga ttcacaatcg
     7021 tggataaatt agttttagat gcttctccaa aaatcttgag aataaacact ggcaaaatat
     7081 tcataacaat tcccacaatc ataaatataa ttatatacaa tttagtattt ttattaaaaa
     7141 attttatata tggctttaat tcattcaatt acaattcctc cttcttagtc atgttaacaa
     7201 acatatcttg tagccctatg tatcctacat caatatttga atttttaagt attttttcat
     7261 cattttcact aaaatcacca tagtaaaaat aagcaactgc atttccaaat gcctttttag
     7321 gttttatatt ttttatacat tctaattttt ctaactcttc tttatttcca gtaatataat
     7381 gagctttttg ttttatgaca tcaatttctt catctataat aatttttccc tcatttaata
     7441 ttattacatg ctccagtaaa tcatctattt catcaatcaa atgagttgat attattattg
     7501 tccttggatt ttttatataa ctatctaaaa ttatgttata aaactcttgt ctatttactg
     7561 catccaaacc aattgttggc tcatcaaata tggtgatagc tgaattagaa caaataccta
     7621 ttatatttga aagtatagtt ttcatacctc ttgacagttg tttatacttt tttttagtat
     7681 ttaaatcaaa atatttgcat aattcttttt ctaagccttt atcataactt ttataaaaac
     7741 tagaagaaaa ttcaaatatc tgacctactt taaaatctgg actataaaat tctttttccc
     7801 tgacaataca aacttcttcc aaaactttta tatcttcttt tgggttcttt cctaatattt
     7861 ctatctcacc ttcattttga attaactgat ttaccattat atttaataat gttgtttttc
     7921 cagctccatt cttacctaat aaaccatata tcttattttt ttcaaagttt agagacatat
     7981 tattaaaaat attttgcttc ttaaaactgt gagataaatt ttctatctta atagaactac
     8041 tcatatatta atcctccttg aaattaatta taatatcaac caattcttct ctatttattt
     8101 ctaatttgtt agcttcttga agtaggtttt ttacaaaatt atgtttaaaa ttttcttttc
     8161 tagcttcttt tataatagtt tttgcaccat ctgatacaaa catccctatc cccctcttct
     8221 tatataaaat atttttatca accaatatat ttataccttt taatactgta gctggattaa
     8281 ttttataaag ctttgaaagc tctgttgttg atggaacctg ttcttcttct tttattccat
     8341 ttgatagtat ctcatcttca attgctcgtg ctatctgaat aaatataggt tcttcattat
     8401 ttaatactaa tttcataggt tcctcctttc tccattagtt agttactcaa ctaattaact
     8461 atataactta taatatcttg aaaaatattt tctgtcaata ctaaataaaa aacagaggga
     8521 aaattccctc tgttttttgt tctttatatt tataattaaa ttttgatttt tgtaatccta
     8581 tcccataagt taaaaacttt aataatctgt tgtatcttca ttttttaata ttttacatat
     8641 tgagctagtt cctaaccttt ctgctcctgc ctctataaat ttttctgcat cagaaattga
     8701 tttaactccc cctgcagctt ttatttttac attttttcca acatgctctt tcataagctt
     8761 tatatcttct aaagtagctc cacctgttcc cattccagta gatgttttta taaaatctga
     8821 acctgacatt gttactattt cacacatttt tattttttca tcttcatcta aataacatgt
     8881 ctctataatg acttttaata ctttatctcc tatagctttt tttatttctt ttatttcttt
     8941 tcctatatta tcataatctt tattttttat atctgaaatg tttacaacca tatcaacttc
     9001 atctgctcca ttttcaatag catcttttgc ttcaaatact tttgtagcag tagtttgata
     9061 tcccaaaggg aaccctatca ctgtacatat ctttatttta ccttttagat attctgatgc
     9121 tcttttcaca tatgatggtg gtatgcaaac tgatgctaca ctcatatcta cagcttcatc
     9181 acataaaatt ttaatatctt cccatgtagt tgtagctttt aaaatagtat ggtctactgt
     9241 ttttaatata tgtttcataa aatcctccta aatttataat taatataatt agattacttt
     9301 tttaaatgca attttctctt tgcaatattc agtatcatat caacctcttc aactttaaaa
     9361 atagtcatta gtagtgtata aattccacct ccaactataa ctgaaatagc tagtgagaca
     9421 aactcattaa atgttcctac tcccaaaatt ccaaatataa atttatacgt aaagtatgat
     9481 aaaacaccca taattaaaga tgcaactacc gatttcaaag ttgcttttat tattttatct
     9541 tgtccaaaat atccaacttt tctctttagg ttcaagaaga gtaacaatat acatgcaatt
     9601 gatgatgaac tagttgcaag agcaagacca ccatgagcca ttggttttat taaaactaag
     9661 tccaatacta tatttacccc tacagatata atcccattta ccataggtgt ttttgtgtct
     9721 tgtaatgaat aaaaaacctt tcccaaaata tctcttaagc caaatgcagt cataccaact
     9781 gcataaaaaa ttaaggcagt tgctgtcatt tgggttgccc tcgcatcaaa agcacctctc
     9841 tcaaaaatga ttctaacaac aggagttgca aaaaaaatag aagcaactga aataggaatc
     9901 atagaaatta ttatcatatt tatactagat ttaactgaac tagtaaactt cttttgatta
     9961 ttttctgcac ttaacttaga taacatagga tacataacag atactattga agctgtaaat
    10021 gtccctgtaa caaaaccatt taatctatct gcataagtaa gtgctggaat gcttccttta
    10081 actaatgttg aggctaaagt tgtatctacc aatgagttca cttgattaac agcaacacct
    10141 ataaatactg gtgaaagtaa tgctagtaat ttaataaggc tatcatcttt aaagtttaag
    10201 tagtataagt atttgtagtt cgttttttta acaaaaaaca tgtaaaagag cagttgaact
    10261 accatagcaa ccacagcacc aattggaagt atgtatggtc caaatatggt acttaacatt
    10321 attgaaataa ttattactat attatagggt atacttccaa atccaactac aataaagttc
    10381 tctttaattt gtaaaaatgc tgacataaca cttgtaatac ctatgaatat tattcctgtt
    10441 ctgaacctcc catgactaaa gtcacagggt tcctgcttca tagaattttg catactaaaa
    10501 tatgtcttac taattctcca caggctatcc ccgtagttcc tacggttctt acatacgtta
    10561 tttattttga attgttatta gtcttaatcc ttcttttaat atatttctac ttgcatttat
    10621 atctctatcg tgagtttcat tacaactagg acaaatccat tctcttatat ttaagttctt
    10681 aacttcctcg tttttatacc cacatttatt gcatatttgt gaacttgcaa agaatttacc
    10741 tacctttaca atttgtctac catgccaatt agccttgtat tctaattgac gaataaactc
    10801 tgaccatgat acatcagaaa ttaagcgaga tagtttatga tttttaatca tattctttac
    10861 ttgtaaatct tctatgcaaa taatatcatt ctcttttatt aatttagtag ttaatttttg
    10921 tagaaaatca tttctttgat tagctatatg ttcttgaagt cttgcaactt ttaatcttgc
    10981 tttatttcta tttaaactac cgattgtttt tctcgataat tctctttgta atttagcaag
    11041 tttgtttaat gacttcttaa aatacttagg attttctata aattctccac aacttgagat
    11101 acaaaattct ttaattccta aatctaaacc tatctgattg ttagtatttt caaatacttc
    11161 aatatctaca tcagtacagc ataatgatac ataatatcta ccacttggtt ctttagatat
    11221 agtagcatta agtattctac cttgaggtac ttgtttatcc cttatcttaa ccataccaag
    11281 tttaggtagt tttatatgtt gaccacaata cataatgttt ccatttgtaa agttagtttt
    11341 atatgaaaat ctattaattt tctttgattt aaatttagga aatcctgctt tttctttaaa
    11401 gaatttttta tatgcattat ctaaatcttt taaggcgttt tgtagtgaaa atttgtcagg
    11461 ttctttaagc cattttaatt ctttttttaa attggtcaaa tcagaactac actgtttata
    11521 agtaaaagtt
//
LOCUS       DAEMGG010000125        11407 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEMGG010000125 DAEMGG010000000
VERSION     DAEMGG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.126"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            251..961
                     /locus_tag="KPK24_003104"
     CDS             251..961
                     /locus_tag="KPK24_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300753.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKNTAVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
     gene            1100..1228
                     /locus_tag="KPK24_003105"
     CDS             1100..1228
                     /locus_tag="KPK24_003105"
                     /inference="COORDINATES: protein motif:HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300754.1"
                     /translation="MEYIQFICKNTVVINGKDIPVSKYRMANLKKKLTYVLGDVLC"
     gene            1222..2541
                     /locus_tag="KPK24_003106"
     CDS             1222..2541
                     /locus_tag="KPK24_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2300755.1"
                     /translation="MLNINVFWIFLPIFSTTIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILSDKKSICDMNNIYFLVDINFLECNFIEMPDI
                     CSIFSNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKIDEVVLKNNKVI
                     TSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
     gene            3007..4305
                     /locus_tag="KPK24_003107"
     CDS             3007..4305
                     /locus_tag="KPK24_003107"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2300756.1"
                     /translation="MIDSEEMIFNVEDLIIYRKNQLVIIYNKKFRKYIKLSEEIYKYF
                     LLGQENKIKIYDFVNYFDDESDKKYIINLIDIMNKNQIFKSNNLNFDMLSNGVAIYFC
                     LTNRCNLFCKHCCVESSSNKKDLLSTYDLKNIVDIISVLNTPHIIITGGEPLIREDFF
                     EIIKYIKQKLPDTKLILSTNGTLIDEHNVDFIITNFEKIDISIDGVDENTCSETRGKG
                     VFNKVISSVNLLKDNGFSNIKLSMVFGQKNSHLNKQFKDLNKKLGTEFVERYFIPTGR
                     GLENKESYLIEDTVLPITIPYILDNELKMCKDKKNKKMGCCACNAGNSQIFINYDGDV
                     YPCPSLIKDDYKMGNIFDNEFVKVIKENKTRDLKSLIKLESIYPYNFEKCKDCDVNIF
                     CWHCPAILDSVKDNELELDRWCSMMKHNLNTAVWGDEKVN"
     gene            4302..5318
                     /locus_tag="KPK24_003108"
     CDS             4302..5318
                     /locus_tag="KPK24_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2300757.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            5374..5535
                     /locus_tag="KPK24_003109"
     CDS             5374..5535
                     /locus_tag="KPK24_003109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300758.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            5743..8289
                     /locus_tag="KPK24_003110"
     CDS             5743..8289
                     /locus_tag="KPK24_003110"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2300759.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            8331..9575
                     /locus_tag="KPK24_003111"
     CDS             8331..9575
                     /locus_tag="KPK24_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2300760.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            9623..10612
                     /locus_tag="KPK24_003112"
     CDS             9623..10612
                     /locus_tag="KPK24_003112"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG2300761.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            10649..11323
                     /locus_tag="KPK24_003113"
     CDS             10649..11323
                     /locus_tag="KPK24_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300762.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
BASE COUNT         4827 a          891 c         1807 g         3882 t
ORIGIN      
        1 catcaataat tttaatgttt aaaatgatat aaaaaattac ctataaatag tatacaaaac
       61 tacattaagt attaattgtt tttaaaaatc taagatatct tcttattttg ttgttaacat
      121 actaaaaata gggggtgatt ttttaagtga ataatagtat ttgatgacca tgttagttgt
      181 ataactaaat aattatttat aaattgatat tttatttgtt ttcagatcta atgtctaagg
      241 aggggtgttt atgtatagaa tagttatctg tgaggatgat attacacaaa tagctttttt
      301 aagagagtgt atattaaagt ccttagaggg tatttctagt caaattgaac tatttgagtt
      361 taattcagga gaagaattat tggaaacaaa tttagaaggt attgatatat tttttttaga
      421 tattaaaatg cttcaactaa caggcatgga tgtggctaaa ataattagag aaactaatga
      481 tacttctgaa ataatattta taacttcaat agttgattac atacaggaag gctataaggt
      541 aagggcttat agatatttat taaagcctat agattttgga gatttaaatg aaagtatttt
      601 gagttgtata tcggatatta taaaaaaaag agaaaatttt atgctgatag aaaataaagg
      661 tataatcaat aaaattttaa ttaatagtat tatgtatata gaggttagga aaaaggtttt
      721 gactatacat actaaaaatg atacttatta tactaaaaat agtatggata aaatagaatt
      781 agagctagaa aaatataatt tttttagatg tcataagagc tatttaatta atttggagta
      841 tattcaattt atatgtaaaa atactgcggt tattaatgat gaagatgtac cagttagtaa
      901 atatagaatg gctgatttaa agaaaaaatt aacatatgta ttgggagatg tattatgtta
      961 gatataaatg tattttggat ttttttacct atattttcta cggcgataga atgaattgta
     1021 ttaaaatttt tatcaaatga attcagtgat ttaagaaaga ataaattttt aaaaatgtta
     1081 caagagctat ttaattaatt tggagtatat tcaatttata tgtaaaaata ctgtagttat
     1141 taatggtaaa gatataccag ttagtaaata tagaatggct aatttaaaga aaaaattaac
     1201 atatgtattg ggagatgtat tatgttaaat ataaatgtat tttggatttt tttacctata
     1261 ttttctacga cgatagaatg gattgtatta aaatttttat taaatgaatt tagtgagtta
     1321 agaaagaata gattaaccct aaatagtagc atagtaattg ctattatgat aattactatg
     1381 ttgaatacaa ttgagtttaa tataaatatt aaattattta tatgtatatt tattacatat
     1441 gtattgtata aaataaatta tgtagtggat aaatggaaaa gtgtatttat aagtttgcta
     1501 tattggatgt tattaattgg atttgatagt attggattaa gtatagtaat gacacttaat
     1561 tctataaaaa atatgaatga tttattagag gataatttgt tgaagttaga gttaataatg
     1621 atatctaaat cattactaat agtattaata ccattgctta agtttatcag attaaaaaca
     1681 aaaataaaca agagggattg tatatattta tctattccat taatagcaaa cattattggt
     1741 gtaatagtaa tatttgaatt tatttttaaa gataagatta tcgattctaa agagagttta
     1801 ataatactta caatatcaac tgtattttta ctatcaaatc tttcattagt aagcataata
     1861 ggaagaataa taaaagataa tagtttaaag atagaatatg aaattacaaa aaaaagaatg
     1921 aatatgcaat ataaatatta tttaaattta caggaatatc agttgaaaat aagaaaacta
     1981 tatcatgata tgaataatca tatcatttgt attcagaatg tttatgggaa aaatgagttt
     2041 gcagataagt atattaaaga tataaataag caaataaaag aatgtaatac tatatttaat
     2101 actcagaata tgattctaga tgttatttta agtgataaaa aatctatttg tgatatgaat
     2161 aatatatatt ttctagtaga tattaatttt ttagaatgta attttataga gatgccagat
     2221 atatgtagta tattttcaaa tataatagat aatgcaatag aagcttgtat taagattaag
     2281 gacacaagta tacaaaaaaa gattaaatta agaggaactg ttataaatag gttatttgta
     2341 ataaaatgtg aaaatacaaa gatagatgaa gttgtattaa aaaataataa ggttattaca
     2401 agtaagaaag attccttttt acatggaata ggaataagta gtgtaaaaag ttcagtagaa
     2461 aaatataatg gaaatgtgga aatatactca gataaaaaca agtttacaat tactatttat
     2521 ataccattaa ctaggaacta attctagttg gggctctaaa tttcccagtt ggagcagtag
     2581 tattgaaaga taaaatagaa gatgctatct ttaaggcata ggatatttgg aggtgataaa
     2641 aatggttgaa tgctatgcta ataagataac atccttttta gtactcaata aaataataga
     2701 acaaaaaatg tgatttatat ttgtttgaat ttaaaatgtt gataggtttt atagttatat
     2761 tatttgcatg atatatctta aataaattta gaaaaataga gttattttta tctgcaaatg
     2821 cgaccttagt atggtaacat tattgaccaa aagttctata tagtataaaa aaataaaatg
     2881 taaatttgat aaactttata actaggaaca taaaaacaac aattaaataa ctaattagat
     2941 agtaaagttg atattagtaa taaatattaa agaaaaatat tttactaatt tgaggaggac
     3001 ttagttgtga tagattctga agaaatgatt tttaatgtag aagatttaat tatttataga
     3061 aaaaatcaac tagtaattat ttataataag aaatttagaa aatatattaa attgtctgaa
     3121 gaaatatata agtattttct tttaggacaa gaaaataaaa taaaaattta tgattttgta
     3181 aattattttg atgatgaaag tgacaaaaaa tatattatta atcttataga tattatgaac
     3241 aaaaatcaaa tttttaaaag taacaattta aactttgata tgttaagcaa tggtgttgca
     3301 atttattttt gtttgacaaa tagatgcaat ttattttgta aacattgttg tgttgaatct
     3361 tcttctaata agaaagattt acttagtaca tatgatttaa aaaatatcgt agatataata
     3421 tcagttttaa atactcctca tataattata actggtggag aaccattgat aagagaagat
     3481 ttttttgaaa taattaaata tataaaacaa aaattacctg ataccaaatt aatattatcc
     3541 acaaatggaa ctttaataga tgaacataat gttgatttta ttataactaa ttttgaaaaa
     3601 atagatatta gtattgatgg agtagatgaa aatacttgta gtgaaacacg aggaaaaggt
     3661 gtatttaata aagtaataag ctctgttaat ctgctaaaag acaatggatt cagtaatata
     3721 aagctgtcta tggtatttgg acaaaaaaat tctcatttaa ataaacaatt taaagattta
     3781 aataaaaagc ttggaacaga atttgttgaa agatatttta ttccaacagg aagaggccta
     3841 gaaaataaag aaagctattt aattgaagat acagttcttc caattacaat tccgtatatt
     3901 ttagataatg aattaaaaat gtgtaaagat aaaaaaaata aaaaaatggg atgttgtgca
     3961 tgtaatgcag gaaactctca aatatttata aattatgatg gagatgtata tccttgtcct
     4021 tcactgataa aggatgacta taaaatggga aatatatttg acaatgaatt tgtaaaagtt
     4081 ataaaagaaa ataaaacaag agatctaaag tctttgatta aattagaaag tatttatcca
     4141 tataactttg aaaaatgtaa agattgtgat gtaaatattt tttgttggca ttgcccagcg
     4201 atattagatt cagttaagga taatgaattg gaattggata gatggtgtag tatgatgaaa
     4261 cacaatttaa atacagctgt atggggagat gaaaaagtta attgaaattt actatatttg
     4321 taactgaaga ctgcaatttg agatgtacat attgttatga aggtaatgat aaaaaaaaag
     4381 aaaatatgag ttatgaaact gctgataaaa caatagattt tatttgtaat aaagaattag
     4441 aaagtaatag tgattataca acaattgttt tacatggagg agaaccattt ataaattttg
     4501 atttgattaa atatatacat agggatttag tagatagatt gagtgacaaa aagatattat
     4561 ttgatataac tacaaatgga actattataa atgatgaaat aatagagttt atagcaaaga
     4621 atgtaaaaaa catatcgata agtattgatg gaaaaaagaa tatacatgat ataaatagaa
     4681 tagatgctaa cggagtaggt acttatgaaa aagtaatgga aaatggaaaa agacttatag
     4741 ataaaggtat atatgttagg gcaagaatga catataactc aaatactgtt aagtatcttt
     4801 atgagagtgt atatgatata gcaaaacaag ggtttaaaaa tatcgcacca atttcagatt
     4861 ttatggataa aaattgggat aattctcata ttaaaatatt aaatgaacaa attagtaaaa
     4921 ttatgaatat taaaaaggat tatcctgata tagatatatc tattttagat aaacaattta
     4981 taaataagaa aaaaggagat tgttttggag ggataaatag ttatgcaata gatactagag
     5041 gagatgtata tccatgtaca gtatgtgtag gagataaaga tattttacta ggaaatatat
     5101 atgatggatt agatgataaa aaaattaaga accttataaa aatttatata tctaagaatg
     5161 aagagtgtga tggatgtact agacttgatt attgtatgtc aaccagatgt aaacttatca
     5221 ataaaaaggt aacaggtaaa ttccaaagtg cagtaccaat ttattgtgga ttagaaagag
     5281 tttatgtgga aacaacaaaa aaactatgta gtttataatc tagtttaact agttataata
     5341 aatataaaac aaaagtaaag ggggatatgt attatgatac atataaaaaa aatcgaagaa
     5401 aaaatagaac ttcaagatgt tgaggtgcaa gcagatccat gtaaatggta ttatgataag
     5461 catggaataa tttcacaaat agactgtatg gtggattgtg caggatcaac tccaaaagga
     5521 acagtagctt attaaaaaat actaatcagt ctattaaata tgttgaaagt actagatgta
     5581 gttttttaca aatgaaatgt gattttctca actctagtat ttttaataat taaatttaac
     5641 tagcacaacg agttagttag tatttaatga atagtgctct ataacctact attttaggtt
     5701 atagagtttt tcttgtaaat atgaataagg aggattctga ttatgataaa tattaaactt
     5761 gcggtgtcat atttaaagag acaaaaagga aaaactttat cattaatact tagtataggg
     5821 attgctgtta tgctagtttt ttcattaagt gttattaatg aatctcaagg acaatcagat
     5881 ataaatcatg catacaaaaa ctcgggagat tatcatgctt tttatgaaga tataaatagt
     5941 gatatagtaa aagaacttaa tggtgaagaa gatataaaag aattcaatga tgtacttaaa
     6001 tttggagaaa ttataagtaa agataatgga gcatctctaa atttatgctc ttacaataaa
     6061 gattttttag attctactag atataagtta gttaaaggta gagagcctag aacttctaat
     6121 gaattagtaa tagaacaaaa agttttagac aaaatggaat tagatgtcaa attaaatcaa
     6181 actattgatt ttcagattgt taatgagcat ataaataaaa ataatgaaaa ttctatttat
     6241 agtaaagata agcaatttaa aatagttggt attttaagta aatctgatga atactatgaa
     6301 aatttaaata aatataaggt aagagctttt acatattcaa acaatggcaa agatattgtt
     6361 ccatatgaat taataactca tatgggtaca ttaaaattga atacaaaaga tttagattct
     6421 caaaaagtag tgaatctagg gagaaaatat aagttaacaa gtaatcaatt ttatccaaat
     6481 acagaagtaa gtatgtctat gtatatgaaa aatatttcaa aaaaaacaac ttattcaata
     6541 aagcaaagct tactcccttt attatgtgca acattagtaa tatacaatat gtttaacata
     6601 ataatactag atatgaccaa gcaaattggt ttacttagag ctgttggagc tagcaagaga
     6661 aatattagac aggtattttt tattcagagt attgtagttt tatctgtagg tatagtaatt
     6721 ggtttacttg gaggtatagt tttttcttac ttaggtctca tgatagtata tgataaaagt
     6781 gcagaattaa ctataaatac aaactctata attgaatcta tcatcatgtc aattatagca
     6841 gttatattag cctctgtagt accaatatat aaagctggaa atttatctat aattgatgct
     6901 ataaaaaaga cagataagtt aaattatatt cctaggcttt tgttgttaaa atcacataaa
     6961 aaagttgttg gaattataaa tgagatagct tttaaaaata tatttaggaa taaggttaca
     7021 tcaataataa taataattac gatatcacta tgtggggttt tatttatagg cagactaact
     7081 tctacaaaat tcatatatgg aaaagagtca gattcagcta atataacaag taaatcatat
     7141 ggcaattttg atatttattt gggatattca ccaataaatg caaattatac attttctaaa
     7201 tatgataatt ctttattaaa tgaaataaaa gctattgaag atgtaacaga tgttgaaccc
     7261 aatatttatt taaaaggata tctaagaaat gctgacttag aagaagatta tttagatgag
     7321 ctaaaaagaa ctgaaataaa taataataat gagtctacag ttttaattcg aggttacaat
     7381 aaagagctat taaataatat agataaatat atagataaag gcgaaaatgt ttatagttat
     7441 acagacaggg attataaaaa tgttttattg gtaaataatt tttattctag aattaaattg
     7501 tcaaataata ctcagattat aaaatcacct aaaataggag acttaataga tataaaaatt
     7561 cctgtatata aagatggaga gtataaatat ataaatacaa aagtaagaat cgctggaata
     7621 atgaaaaata gctatatatc agagcaagat ggagaatctc aatcaggtgg aattcaaatt
     7681 atatttaatg aaaaggattt aaaagaatta acagggataa gtgattataa taaagttttt
     7741 gtgaagataa aaaaagaaac agataaaaaa gtaataaaag aaataaataa tataatagaa
     7801 aaaagtggat tttcagatat agagggaaga tatgtacgta atcattttgg agaccattat
     7861 aataaatcct catataaatt agcaatgatt tgtgtatttg gagttttatt aatatcatct
     7921 ataaacatta tttttatagt tagatcaaat ataattgtga ggttgagtga gatatgtaca
     7981 tttagagcaa tagggatgtc caaaaaaaat gtaaaaagaa tgttaataaa agaaaatatg
     8041 atatatgggg tattaagtat gattgtagct ggaattatat ctagtttcaa ttattataaa
     8101 atagtttctg aaactaacaa acttaatcaa caggtatatg gtataaataa ctctattaaa
     8161 tttgaaattc caatatatga aattctaata tttggatcta tatctgtttt tgtttgtgtc
     8221 attgcagttt atttttctaa taaaatgata aataaactta gtatagtaag tggtataaaa
     8281 gaaaattaat gaaatttcta atttaaatat ataggaagga aataaaataa atgaatagct
     8341 caaacaaata ttttttagat tgtatatgtg aaaaaataat aaataataat caagttattc
     8401 ttagtaataa agtaaatgga agatgggtta aaattccaat agaatgttat gaagttctaa
     8461 agtattcagt agataatgca ttacaacttg gtaatttgta tgatgtattc caagaaaatg
     8521 aagataaaaa atattatgaa cgagttatag ataagttaga gcatatagga cttctaagtg
     8581 attctattct aagtgaattt agactagaga gaataaacaa aatttcattt tctccaacta
     8641 atagatgtaa tctaagttgt gattattgct gtgtagattc taaaatagat aataacgatt
     8701 atttagatac atctcaagta agaaaagtaa tagataatat agttaaatta aatccaagga
     8761 tattaaccat tacaggtgga gagccattga tgagaaatga tttcttagag ctgttggatt
     8821 atataaaaga aaaatttaaa ggtaaaataa ttctgtctac aaatgcaact ttaattaaag
     8881 aaaaacagat tgatgatata attaatggtt tatatgcaat agaaattagt ttagatggtt
     8941 atgatgaatt aagttgtagt gaagtaagag gtaaaggagt attctgtaaa gtattaaatg
     9001 tgattagact tataaaacta aaaaaattcg agaacgtagg tttgtctatg gtggtaggaa
     9061 agaataatga gaaagacata gagaaattca ataaattaaa tgaagattta ggtacaacgc
     9121 ctataataag aaattttatg aatattggta gaggtaatga gaactatagc aaatatctta
     9181 atgataaact tgatattgat tacatttgca aagatgatta tgtggatgaa agtagtttat
     9241 gttcaaatat gtgtaaagca ggtgttaatc agataagtat agatcataaa ggagatgtat
     9301 atccgtgtcc aaacttagag tataaggatt taaagatatt caatatatta gaatttaatg
     9361 aaaacatatt agagaggatt cttaatagag aaatggatat atttaatata tttgataaat
     9421 taaaaccagt aaatattgag agttgtaaag aatgtaaagt aaatttattt tgtacaactt
     9481 gtccatcgaa aatatatact ttaaaaaata acaaaaaaat gtttgatgca aattgtaaaa
     9541 aaatgaaaaa catcttggaa cctatattat ggtaaggatt agttttatat tcaaggtaat
     9601 ttattttaga ggtgattatt aattgactaa agtaagaata gcagtcttag acacaggatt
     9661 agatgtaaat gatgatgata ttaatggaaa ttatgtttat gattcaaatt ttcaattgaa
     9721 agatttgggg gggaaggaaa taaatgacat aaatggacat ggaacttttg tatcgaaaac
     9781 catattaaat ataagtaaaa atacccaaat ttatcctatt aatatattta atgattctgg
     9841 aaaaacaagt tctttaaatc ttcttagtac actgagcaag ttgactatgt cggatataca
     9901 gataataaac atttcagctg catctattaa tagtatgtat gaaaaagaat tagaaaaggt
     9961 ttgtaatatt ttaaatagtc aaggtagatt tattatttgt tcaaaacata ataaatatga
    10021 agataaagaa agtattccta ctatatttga aagtgttatt ggtgtagtag gtaatgaaaa
    10081 tatatataaa aataatcaat atatatacga accagaaaat aagattcaaa tgtatgcaaa
    10141 ttcaaaagat aattttatta aatttaaaga taaagtaact cattttggta aaaatagtaa
    10201 ggcttgtgca gtggcaactg gcattgttgc aaatatatta aaaaacaatc catatattaa
    10261 gtttaatgaa ttggaagaaa tacttattca aaaaagcaaa agtaaagaag atttaaaaat
    10321 agaaaacttg aacactccaa gttataaatc taatttgata agattggata tagcaaggaa
    10381 aataataaat gtgataaata gaaacttttc taaaataaaa atagactttg aatttgtaag
    10441 tgaatattct ttatttaaca attttacatg tattggaaat tataatgcat ataatttttt
    10501 aactatttta aataaagagt ttaatattag tattgactac aaagaagtat acttatatca
    10561 attagagaat ttagatatgg cagtagatct tatttacgaa aatttaattt aaaaatatat
    10621 gttttttaaa tgggaatgga gatacgttat gaaaatactt aaggtaaaag atttaaaaaa
    10681 aatatatgga aaatatgaga atgaagttag agctcttgat ggtattgatt tagatataga
    10741 gccacataaa tttacagcta ttgtaggtgc aagtggttct ggtaaaagta cactacttca
    10801 ctgtgttgca ggcttagaca agccaacttt agggaatgtg tacttagatg atgtagattt
    10861 atatacatta aatgatgata agctgtctga aataagaaga caggaatttg gcttcatatt
    10921 tcaaagttac aatttaatac cagttataaa tgtatatgat aatatagtac ttcctgtgtt
    10981 gcttgatggg aaaaaagaag ataaagaata tgttatgaga ttaattaata agttggggat
    11041 tgaaaaacaa ataaagaaat atccgaatga actatcagga ggtcaacaac aaagagttgc
    11101 aatagctaga gctttagcca atagaccgtc agtaatattt gcagatgaac ctactggcaa
    11161 cttagatagt aagactacaa atgaagttat gaatatgctt gaatggtgtg ttaaagagta
    11221 caaacaaaca ctagttatga taactcataa tgataatatt gcaaagacag ctgatagaat
    11281 tataactata aaagatggaa aaatagatga aaaaaactat taataataat ttatttagaa
    11341 tactaaatat aatagtatat aagaataaga ctaagttgat agtatcattt ataatggttt
    11401 ttataat
//
LOCUS       DAEMGG010000126        11136 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEMGG010000126 DAEMGG010000000
VERSION     DAEMGG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.127"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            58..825
                     /locus_tag="KPK24_003114"
     CDS             58..825
                     /locus_tag="KPK24_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBG2300763.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1043..1256
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1510..2544
                     /locus_tag="KPK24_003115"
     CDS             1510..2544
                     /locus_tag="KPK24_003115"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG2300764.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKIASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2585..3802
                     /gene="argJ"
                     /locus_tag="KPK24_003116"
     CDS             2585..3802
                     /gene="argJ"
                     /locus_tag="KPK24_003116"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG2300765.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEAHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3817..4677
                     /gene="argB"
                     /locus_tag="KPK24_003117"
     CDS             3817..4677
                     /gene="argB"
                     /locus_tag="KPK24_003117"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG2300766.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4707..5894
                     /locus_tag="KPK24_003118"
     CDS             4707..5894
                     /locus_tag="KPK24_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG2300767.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYVERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6003..7025
                     /gene="argF"
                     /locus_tag="KPK24_003119"
     CDS             6003..7025
                     /gene="argF"
                     /locus_tag="KPK24_003119"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG2300768.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7152..7769
                     /locus_tag="KPK24_003120"
     CDS             7152..7769
                     /locus_tag="KPK24_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG2300769.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(7989..8687)
                     /locus_tag="KPK24_003121"
     CDS             complement(7989..8687)
                     /locus_tag="KPK24_003121"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG2300770.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8849..10066)
                     /locus_tag="KPK24_003122"
     CDS             complement(8849..10066)
                     /locus_tag="KPK24_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBG2300771.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(10257..10670)
                     /locus_tag="KPK24_003123"
     CDS             complement(10257..10670)
                     /locus_tag="KPK24_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2300772.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
BASE COUNT         4386 a         1203 c         1832 g         3715 t
ORIGIN      
        1 ataaaaatat atagaatata tttaaaaatt attatgcaat tagaaaagga gataagtatg
       61 cagattattg atataaatat aaataatatt gacaatgaac atatatgttg ttcaatttct
      121 gaaaaaaaag gagaaaattg tgttttttct aaaaaagctt ggatgaaaaa taattttgat
      181 aaaggtctag tctttaaacg atttgatgta agaggtaaag cttttataga atatattcca
      241 gcagaaaatg catggtgtcc tattactgcg gacaattata tatttattaa ctgtttttgg
      301 atatctggaa aatttaaggg tcaaggatat tctaatctat tgctacaaga gtgtgttgat
      361 gatgcaaaat caaaaggtaa atatggatta gttgtgcttt cgtctagtaa aaaaatgcct
      421 tttttatctg atgctaaata tctaaaatat aaggactttt gtttagcaga tacatcagaa
      481 ccaaattatg agctattata ttttcccttt actgataatg ctcctattcc atgttttaag
      541 aattgtgtaa aacaaagaaa attaaatgag aatggtatag ttttatatta cagtaatcag
      601 tgtccacata cggaaaaata tgctcctatt ttgaaaaaaa ttgcaaattc tagaggtatt
      661 gatgtaaatc ttataaaatt cgaaacagca aaagaagcac aaaatgctcc tgcaccattt
      721 actacataca gtcttttttt taatggtgaa tttgtaacca acgagatact ttctgagaaa
      781 aagattgaga aatttttaga taccaacatt aaaaaattta attaaaaaga gatgtaaata
      841 ttgtattttg aaaatatatt attttataat ttaatatatt ttggatgttt tttaaattga
      901 cgaatgaaat ataaaaatta tatcgcaatt tagatacaaa atgctggaaa ttaaccatat
      961 agaaaatatt tccaaaaatt taaaaattat attgacaaag ttaaaaaaat aaactatcat
     1021 ataaaacaat aagaaaataa attgaaatcc tgtgaaaaag agagtagcat aaagctagtt
     1081 gatagagagc tgggaatggt ggaagcctag taacgaattt tattgtgaat agagctttgg
     1141 aggagattac cagaagtaat agtatggtaa ttggtgaatc tcgccttaag agattagagt
     1201 gggtaagatt atcttatcaa ttagagtggt aacgcgggtg tatattcgtc tcttggtctt
     1261 tgaccaagag gcttttttaa ttttaaatgt acattttgta gatacttttt aatttgagtt
     1321 ttaatgatta ttgtaatttt accaataaga gtggtaccgc ggaaaatcgc ctcttagtat
     1381 ttataatgct aaggggtttt ttgttttaat tagagtattt taatctacgt attatcaaat
     1441 gattatcata tagtaaagtg gaaagatagg attttaagac tatttgaata taaaaatgga
     1501 gggaacaaaa tgaataaaat aaaagttggt attattggtg caactggata tgtcggggca
     1561 gagttgatta gattattaat gaatcatgat aaagtcgaag tgacagcgat aggttcaaat
     1621 tcgtatgttg gaaaagatat agttgatata tatccaagta taggatataa aaataacatg
     1681 atatgtatag aaaatgaaaa agttatagat atgtgtgatg ttgtgtttac agcccttcca
     1741 catggagtta gtgaaaagtt tgtaataaag gcaattaaat cgaaaaagaa agttatagat
     1801 ttaggtgctg actttagaat aaaagatgaa gaagtatata gtaaatggta tggagtatct
     1861 tttatagata aaatcttaca taaaaaagct gtatatggat taagtgaaat atacaaagaa
     1921 gatataaaag atgcagatat aattgcaaat cctggatgtt atccaactag tatttcttta
     1981 ccattgatgc cattactaag ttctaaattg ataaaaaata ataatataat aattgattct
     2041 aaatcaggtc ttacaggtgc tggtagagaa ttatcgatta gtagtcactt tacggaggtt
     2101 aatgaaaata tcactgcata taaaataggg aaacacagac atactccaga aatagaacaa
     2161 aatttaagtg aatcctgcaa agaaaaagta agtgtagttt ttacaccaaa cttaatccca
     2221 gtaaataggg gaatcttatc aactatatat tgtacaaaag aagataatat ttctataaat
     2281 gatatacata ggaaattaac tgattattat gaatttaaag aatttataga aattcttcca
     2341 ttagataaga tagcttcttt aaagaatgtt agattctcaa ataaatgtgt aatatcatta
     2401 catgaaaatg gagatacatt gataatctgt tcagctatag ataatatgat taagggagca
     2461 gcaggtcaag ctatacaaaa catgaatata atatttggta ttgaagaaaa tacaggttta
     2521 aaaaatattg caccatcatt ttagaaataa aaaatactaa actcatataa ttaggaggat
     2581 aaatatggag attataaagg gtggagttac agtttcagaa ggattttttg cttcagggat
     2641 tcactgtggc ttgagaaaaa ataaagaaaa aagagattta gctttggttt attcagatgt
     2701 tttatgtgat gcagcagcag tagtaactca aaataaagta aagggcaatc ctgtttatgt
     2761 tacacaggaa cacattaaaa atggaaaagc acaagctatc atagtaaata gtgctaatgc
     2821 taataccttt aatggaaaag aaggacttat aaatgcttat aaaatggcta aattcaccag
     2881 tgataaatta aatattaaag aaagtgatgt tttggttgca tctacaggtg ttataggaaa
     2941 gccattaaat atagatttaa tagaagaaaa tatggatgaa ttagtgaata atctaagtaa
     3001 acaaggtcat attggagcaa gagaagctat tatgacaact gatattataa aaaaagaaat
     3061 agcagtagca ataatgtatg gagataaaaa aataactatt ggaggtatgg caaaaggttc
     3121 tggtatgata catcctaata tggcaacaac gttaggattt ataactacag atgcaaatat
     3181 tgatggagtt ttgctaaaag aagctttaaa aattgctgtt gacaaaagtt ttaatagagt
     3241 tagtgtagat ggagatacaa gtactaatga tatggtaatg attctagcaa atggaaaagc
     3301 aaaaaatgat agaataaaca aaaaagatga acattatcaa gtatttttga gtgcacttac
     3361 atatgtctgt atagaacttg ctaaacttgt tgcaaaagat ggagaaggat gtacaaaact
     3421 aattgaatgc tatataaatg gagcattaag tgaagcacat gcagtaaaat tagcaaaaac
     3481 agttatatct tcttcattag taaagacagc tgtatttgga gctgacgcaa attggggtag
     3541 aatactgtgt gctttaggtt atgcaggtga agaaatagat atggaaaaag ttgatattat
     3601 ttttgagagt atgaaaggat atattgaagt atgtaaaaat ggaaatggat tagattttaa
     3661 tgaggaaaaa gcaaaaaaaa tccttgaaga tgacgaaata agtatattag tagatttaaa
     3721 tatgggcaat gccagaggaa atgcatgggg atgtgattta agctatgatt atgtaagaat
     3781 aaatggaagt tatagaaatt aggaggatat actttaatga ttaatataga aaaagcaaat
     3841 acacttatag aagctttacc atatatagaa aaacatcagg gaaagactat tgtagttaag
     3901 tatggtggaa gtgcaatgaa aaaagatggt ctaaaagaat ctgttatgga agaccttgtg
     3961 ttaatgagtt atgttggaat caatatagtt ttagtacatg gtggaggggc agaaatcaat
     4021 aagatgctag caaaagtgga tatagaaagt aaatttgtaa atggtcttag atatacagat
     4081 gaagagacca tggaaattgt aaaaatggta cttgctggaa aggttaataa agacctagta
     4141 aataaaatac atacaaaagg tggaaaagca gtagggcttt gtggaataga taataatatg
     4201 attttatgtg acccatataa aaattatgaa cttggatttg taggagaaat caaaaaagta
     4261 aatgttgagc ttatagaatc ttgtctaaag tcaggttata tttcagtaat tgcaactata
     4321 ggtgttggag atgatggaga gacttataat ataaatggtg acacagcagc ttctgctata
     4381 gctaaagagt taaatgcaga taaattgata cttcttacag atgtccctgg tttgttaaga
     4441 gagccagatg aagaaaaatc attaataaca gaagttatat tagaggatgt tgataaatta
     4501 tttgaagaag gtataattac tggaggtatg atacctaaaa tagagggatg cgtagatgcc
     4561 ttaaataacg gagtaaatag ggtacatatt ttggatggaa gagttccaca ttctataata
     4621 acagagctat ttactgatag tggaataggt acattaataa gaaaagaaaa tgaataaaat
     4681 tataagttta agaggggaga ataagaatga ataacgacaa tacaatttca aaatggaatg
     4741 aatattttat agatacatat aatcaaccca attttgtcat agattatggt gaaggaagtt
     4801 gtttttttga tactaatggc aataaatata tagattttac aagtggatat ggagtatctt
     4861 ctcttgggta tagtaatagt aatttaaaga atgctctaaa agagcaagta gataaattat
     4921 tacatacttc aaatttatat tttaatgagc cagtgttgta ttcaggagag aaaataatta
     4981 attcaagtgg aatggctaaa gtatattttt gtaattctgg aacagaagca aatgaaacag
     5041 cattcaaaat agcaagaaag tatagtagtg acaaatatgg taatggtaga ggaactatca
     5101 taagtttaaa agactctttt catggtagaa caatgatgtc tttaatggca actggtatgg
     5161 acaaatacca taagtatttt tatcctcttc ccgaaggttt taaatatgta gaaagaaata
     5221 atattgaaga tttaaaaaat aatcttgatt ccacagtttg tgcaataatt cttgaagcga
     5281 ttcaaggcga aggtggagtt aatgttcttg aaaaagatta tgttttggaa atagtaaaaa
     5341 tatgtcaaga aaaggatatt gtagtgattt ttgatgaagt ccaatgcggt ataggaagaa
     5401 ctggtaaact ttttggctat gaatattttg atgtaaaacc agatatagtt accgtagcaa
     5461 aagggttggg agcaggaata ccagtaggag gagtattagt aaataaaaaa ctttcaaaag
     5521 tactaggaaa aggagaccaa ggtacaactt ttggaggtaa tttattagct atggtggcag
     5581 cttcagttgt attagatgag atatctaaag atggttttta taatgaagta ttagaaaaag
     5641 gaaattatat aagaaaatct attgaaagtt ttaataacaa agttgtatta aagacaaaag
     5701 gtattggtct aatgatagga attgaaacta atattgaatc tagtattata gaagagaaag
     5761 ctagaaaaaa aggattgctt attttgactg ctggaaaaaa tgtattgaga tttttaccac
     5821 cattaacaat cagttataag gaaatagatg aggctcttga aatcctaaaa gatatattac
     5881 tagaaattaa ctaaatattc aagaaatagt tgattaaaga taaaaattag aagtaaataa
     5941 aaaagtaaag tgctaacaag tttaattgaa tttaaaaagt ttataagaag gggcgaatta
     6001 atatgaataa tataagttta aaaggtaaga gttttttgac attaaaggat tttacaaaag
     6061 aagagattag atatttatta actttatcaa gttatttaaa aactaagaaa aaagttggta
     6121 taaaaggcga tttgctacaa ggaaaaaata tagctttatt atttgagaaa acatcaacaa
     6181 gaactagatg ttcatttgaa gtagcagctc atgatgaagg agcacatgta acttatctag
     6241 gtccaaatga ttgtcatatg ggtaaaaaag agtctgtatc agatacagca aaagttctag
     6301 gaagattcta tgatggaata gaatttagag gctttaaaca agaaacagtt gaaagtctag
     6361 caaaatattc tggtgtgcct gtttggaatg gacttacaga tgaatatcat ccaacacaaa
     6421 tactggcaga ttttttaaca gtaattgaaa atgtagataa agatttgaat aaagttaaat
     6481 ttgtatatgt tggtgatgct agaaataata tgggaaactc actgatgata ggatgtgcta
     6541 aaatgggaat acatttcgta gcactagcac ctaaggaact ttggccaaat gaagaattgg
     6601 ttaaggaaat gaaggaaata gccaagaaat ccaaaggaga tataatgttg actgaaaata
     6661 ttgatgatgt taaaggtgca gatgttatat atacagatgt atgggtttct atgggagaag
     6721 aggagcaata tgaagcaaga ataaatcaac ttaaagatta tcaagtaaat atggatatga
     6781 taaagaaaac tgaaaatgaa aatgttatat ttttacactg tcttccagca tttcatgatt
     6841 tagaaactaa ggttgctcag gagatttatg aaaagttcgg attatcagaa ttagaggtta
     6901 caaacgaggt atttaattca aaatattcaa aggtatttga agaagctgaa aatagaatgc
     6961 atacaataaa agctgttatg gtttctacta tgactgatgt aaatatgaac aataatttaa
     7021 tatagatatg ttttaatata gactttactt taatgaaaat ttataaaata ttttaaatat
     7081 attattcaaa aactctcatt atatgttaaa ataacttatg tatcaacaaa ttaagaggga
     7141 ggatttgaga tatgaaaaaa atcatattag ctttaacaac ttttttatta gcaatatctt
     7201 taacagcatg ttcaagtgca aaaccagaag atacaattga tagttttttt aacagtgcga
     7261 aaaagtttga ctttgaaggc atgaataagg taatggaaaa taatgacgaa aaatataagg
     7321 atatattaaa ggaattagat acaaaagacc caaatgccca atatgtttta gattatctaa
     7381 aacagaatgc atctaaaatc acatatacaa taaaagacag cgaggttaaa ggtgataagg
     7441 ctacaatcaa agtagaatgt aaatttatag attcaacacc attacttcaa gaaatagtag
     7501 ctgaggcatt tacaaagatg attggtatga gtttttcagg acaagactta actgatgaga
     7561 aaacaactga aatgttagta tcaattatga aagaaaaaca aaaaagcgtt aaagagactt
     7621 atgttacaaa aaatgtagag tttgaatgtt ctaaaaagga taataaatgg ataataagtt
     7681 cagtaaatga tgcagtagct gatgtattgt tatctaatct tgttacagca ggacaagaat
     7741 tttctaactc tatgaataca cttgaataat attgtttagg tgtagtaaag taataaatat
     7801 atgattattt ttataaataa acatataaat atttatgaag gataccattt ttggtatcct
     7861 ttttattaat ataatgaaat acatggttaa tttctatatt gtcaagaaac tatgatatat
     7921 gttataagtc tatactattt aaaataaact tataacatat attttttata atatagtgca
     7981 tgaaaaattt atttaactaa ttttagatat ttattgacaa ctgattcagc caagcatttt
     8041 gatgaatcaa caatatttat tgaaggatat gatttttcta aaactacatt taaatcagta
     8101 caagcaatta ctatattttc tatagattca tttttaacat cttcgataag tttattccaa
     8161 atatcaatat tgtgtggatt ttctttatcc atttttatat tttgaataag attgtttaat
     8221 tttatttgcc attcatcttt aaatataaat tcatgtccat tatgtattat accattctgg
     8281 taaattgtag actcaaaagt tgaactagtc gaaaaaagag ttactttttg tgagatagta
     8341 ggtaattttt taactgtttc ttctacaata tttaatattg gtatattaat agactctttt
     8401 aattctttaa agtatatatg tgctgaattg catggcattg caataaaatt tacaccagtt
     8461 gattcaagct tttgtaatcc atcaattata gtttctttca taagttcatg atttattgga
     8521 cggtcaatat aaaatggggt aggtaatgaa tatatcatca tctttggaaa ttcatcatca
     8581 tattttgctc catattgaga ttgacactca tcaataatta aatcaacaaa aggggctgtt
     8641 gaccttggac ccattccagc caaaatacca attaattttt cattcataaa aattatctcc
     8701 tttatatata aatatattta tgtataaaat aaaaatctat aaaagcataa aattatattt
     8761 tataatagat tatattgtac acttatataa tataaatata aagtgatttt ttagattata
     8821 tattttaata tactttctta tataagaatt attttaaatc aagattatct agtattttta
     8881 aaaatatttt tacagcagta tccaagtcat ctacagaaac actttcatta ggattatgac
     8941 ttacgccccc agcgcatctt ataaatacca taccaatatc agttatatta tccatttcct
     9001 gagcatcatg gccagcacca ctataaatat aaaaaggatt aagatttaaa tctataaaac
     9061 ttttttctat gatttttgtt attttattag aacatggtac aggaacattt tcaaaagcaa
     9121 gttcacttgt ataattaagt tttctatttt cacatatatg tgatatttca ttaaatatct
     9181 tttctacaga attatcaaga atttcttgtg aacaagagcg tacatcaaga gtaaattcag
     9241 ctctttcagg taccacattt gatgaagatg gatagaaatt catctttcct acagtagcaa
     9301 ccaaatcagc agtcgtttta gctacttttt cgatagctaa tacacattca caggcacaac
     9361 atccagcatc aagtctcata ttcattggaa gtgtaccagc atgtccagac ttaccattta
     9421 ctgatatctt aaaactttta aatcctgtaa tacttgatac aattccagca ggaagagatt
     9481 cattttctaa aacaggaccc tgctcaatat gaaattctaa atatgcatct acatcttttc
     9541 taggatgcaa atttgttaag ttttttgtat taaatccaaa ctttgttaca gcttcttcaa
     9601 gtgtaattcc attttcatca acagatttaa gaagttcctc tttaaaagta ccagcaaaaa
     9661 ctttacttcc cattaaagaa gtttcaaatc tagttccttc ctcttcagcg aaagcaatta
     9721 gcttaatatt gaaaggataa gacctattat tgtgttttgc ttcttcaaga ccaactagag
     9781 gcactattat acctaacata ccatcaaata tacctccaca ctttacacta tcttgatgag
     9841 atgcaactac taatgtggga gcatttggaa agcttgattt atatgtacct ataacgtttc
     9901 caatgtcatc aatttcaata tcaagattta aaccttccat ccatgaacta attaaatcaa
     9961 tagatttttt atgttcttga gtcaagataa gtcttgtaag accatttgtt gttgaactta
    10021 tttttcctaa tgtttgtaga taatccatac atttttttcc taacatattt acctcctaaa
    10081 aatataattt ttatttaagc taagattagc ttttatactt ataatttttg aactttcaaa
    10141 taattttgta tgtatagtaa aatactattt tagtattaat aatgatataa aaatataaag
    10201 tctgtagacg aagagcagaa gttttaagtt aaaaatataa aatatgattt ttattattat
    10261 agtatttttc taaaataata tctctttcca gttagaggat aattttcaag tgaaaatact
    10321 ttatggtatc caaatttctc ataaaattta ggtgcttgaa aactgaatgt atctaaaaaa
    10381 acatatttac aacctcgttc tttagcaatt ttttcagcct tatataaaag ttgacttcca
    10441 gtacctttat atctaagctt ctcactaacc cataagtatt ttatagaaag ccaattacca
    10501 tgagtaacac ctataaaacc tgcaacaatt ttacctgctt tatctcgaat atatattcct
    10561 aaatccactg ggtttttatc ttcaagtcta gcaagattat attttaataa tccttgaaaa
    10621 atagtatctt catcttgttt ttctattttt tctgtaattt ctaattccat atttgaactt
    10681 tctccttgta taatcttttg tatattggta ttttttaata aaatcagtag aaagaatata
    10741 gtttaacatt attctaatag gcatttatgc taatgtcaaa ctaataatac ttattcttaa
    10801 ttttatattg ttgatattaa atttttttaa ataaaaatgt agtttaagtt atataaaaag
    10861 aaatcatcaa taaatttagt aaacctaaat tgttgaatat taaaataagt taatttaata
    10921 cataaaaact attttagatg ttattctatt gctatgttca ataatatcat tagtagttca
    10981 atttataaaa tgaataaatt atataaataa gattagaaag tagtaaatag tcttattaaa
    11041 tataagtaat aaaataataa atacctagta gagattaata attaaaaaat aaaatactac
    11101 caaaataaac tcgatagtat tttatttaaa tactta
//
LOCUS       DAEMGG010000127        10913 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEMGG010000127 DAEMGG010000000
VERSION     DAEMGG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.128"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            153..1853
                     /locus_tag="KPK24_003124"
     CDS             153..1853
                     /locus_tag="KPK24_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300773.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            2023..2640
                     /locus_tag="KPK24_003125"
     CDS             2023..2640
                     /locus_tag="KPK24_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300774.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            3001..4194
                     /locus_tag="KPK24_003126"
     CDS             3001..4194
                     /locus_tag="KPK24_003126"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG2300775.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4212..5504
                     /gene="lysA"
                     /locus_tag="KPK24_003127"
     CDS             4212..5504
                     /gene="lysA"
                     /locus_tag="KPK24_003127"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG2300776.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYVEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            5721..6380
                     /locus_tag="KPK24_003128"
     CDS             5721..6380
                     /locus_tag="KPK24_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300777.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNSNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6535..7620)
                     /locus_tag="KPK24_003129"
     CDS             complement(6535..7620)
                     /locus_tag="KPK24_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2300778.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7811..8527
                     /locus_tag="KPK24_003130"
     CDS             7811..8527
                     /locus_tag="KPK24_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG2300779.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8585..9163
                     /locus_tag="KPK24_003131"
     CDS             8585..9163
                     /locus_tag="KPK24_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2300780.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9557..10429
                     /locus_tag="KPK24_003132"
     CDS             9557..10429
                     /locus_tag="KPK24_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2300781.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4501 a         1130 c         1882 g         3400 t
ORIGIN      
        1 atacatacac gtataatgtt aaatatagtt gaataagcag aactataaat atataaagat
       61 atcttaatat ataataaata tgataaatat ttattttttg taaatatatg gatataatag
      121 taataataac attttttaga gaggagtatt aaatgaaacg atatcttaaa acatatggag
      181 ttgcattaat aattgtaata atttatgcat ttattaaatt acctgtacta agattagatt
      241 ttacatcttt attttcagta ggaataattt tctttgttgt agcaggaata ctagatatga
      301 tgttagatag aaatgaaaga gcatctaaaa tggctaaata taatttttca atagcaatag
      361 ctttgcttgt gtatattgta gtagttccct ttataacttc tacacctgta cttcatgcaa
      421 aatcatatag agaactattg ggaaaagtaa ctgaaagtaa atttactgat gacattagcc
      481 ctgtcagtgt aaatgacatt cgtcttgtag atgaagatat ggcaatgaaa ttaggtgaca
      541 aaaagcttgg agaagttcct gcaataggga gtgtatccaa attaggaaaa tttcatatac
      601 aaaatgtaga tggagagttg tactgggtag cgcctcttgt tcacagagac ataattaaat
      661 ggataacatc tctaagtggt acaagtggtt atgtaaaagt atcagctagt aatcctcaag
      721 acgtccaatt agttcaagaa atagatggaa agccaataaa gatagtttac caaccagaag
      781 cctacttaca tcaggattta caaagacatt tatacataca tggtattgta aatgttggta
      841 tgacagattt cactcttgaa ataaatgatg aaggaagacc ttattgggtt gtaagtttat
      901 atgaacataa aattggctat ggaggagcaa atgcaacagg aatagccact gttgatgcag
      961 aaactggaaa aataaatgtt tatgatgtta aaaacacgcc taaatgggtg gatagaatac
     1021 aaccacaaag ctttgtaaca gaccaaatta aagactgggg tgtttatgtt aatggatttt
     1081 taaactctgt tatatcagaa aagggagttt tagtaccaac agaaggtaca tcactagttt
     1141 atggcaatga taacaggtct tattggtata caggtataac atcttcaggt ggagatgaat
     1201 ctacaattgg atttatgtta gttgattcta gaacaaaaga agctagatta tataaacaac
     1261 caggagctac tgaaacggct gcaatgaaat cagctgaagg taatgttcaa gaaaaaaatt
     1321 atgaagcgac gttccctgta atgtataata tattgggtca gccaacatat gtatcatcat
     1381 taaaagataa ggctggtctt gttaagagag ttgcatttgt atctgttgaa gattataatg
     1441 tacttggaat aggtgaagat aagaatgaag cattaagaaa ctataaggat gcattagaat
     1501 ctaagggtaa tgatttaaaa cttgataatg atttaaaaga tgaggtatta gaaggcacag
     1561 tgacaagaat aagcccagat gtgcgaggtg gtaacactaa ttattatgta actttagatt
     1621 caaataaaga tataatattt agggctactt caaaagtatc tagcgaattg ccattgactc
     1681 aggttggtga taaagtcaaa atatcgtatt caaaagaaga atctggtgta atagaaatgt
     1741 cagaatttga taacctaaat attgcaaact ttgaagataa aaaagcaaaa gaaaaaacag
     1801 gaactgatga aaatacagat tccaaagatt ctgaaaatca aggtaatgta taatttaaat
     1861 aaaaagattg ataatagtat ttaacatagt gatagctaat ttttatagaa tttaattcta
     1921 taaaaattag ctattttatt ttaattcttt gtaaatttaa tgtcataaaa ttaatataat
     1981 attagtacaa ctttgcaaat aatacttcaa ggagaatgta ctatgacgaa tatggaaaaa
     2041 gagatattac ttttaattag cagggttaga tgtgttacag agtctcaatt tagaaaaatt
     2101 tatggagacc aaaaaagata caataaaaaa aattttaaaa aaactttgag aaaaatgtgt
     2161 agtgaatata cactaaaaaa atatccttgt gatgtggatt attataatta taaagacctc
     2221 tcttatgtat actatttaaa cggtagtaaa ctttttaagg gagatgattt aataaaggct
     2281 ctagtaggat cagaattagt tgttagaatc aatactgcag gttgtgatgt aaaaagattt
     2341 taccgaaatg ttagtgtaga tgatgtgaaa tacgatttat ttattgaata tacagaccaa
     2401 tttggtaata taaagcaagt tttagttgat gttgacttaa ataatgaaat agacatttct
     2461 aaatacgaaa atctttctaa aaaaatagat aaatctacaa ttccattttt taaagtacca
     2521 aacgttttgg tagttacacc agataatcta tccgaattaa atgatttaga gagatatgat
     2581 aatgagtata gcataaattt tatagattta ggattaaata aactatttaa ttgtatataa
     2641 gctaacttta gaaaagaaaa ctatcgattt gccacatttt gtaaaataat tgttgcaata
     2701 ttatatagaa tatgataaaa ctatattaat aaattaatta aaaataatga gcctagggta
     2761 gaggtagctg tattcaatag tattcaatag gagctggtaa gcgttgataa ttgatgaaag
     2821 ggtatatagc cgaagaaatt ttgttgacgg acaaattttc tggacttatg cagaatatgc
     2881 ataaggctgt cacttttaaa gtggtgagct acaaggttac ttggtctatg aaatttacac
     2941 gtccaaagta tacctttggg cgtttttatt tatcaaaatt tagcttgagg gggagaaagc
     3001 atgagtataa ttgttcaaaa atatggtgga agttcagtag cagatactga aaaaataaaa
     3061 tcaatagcag aaaatataat agaacgaaga aaagaaaatc cacaaatggt aatagttgtg
     3121 tctgccatgg gaaaatccac tgatgaatat ataactttag caaaggaatt gagtaatgaa
     3181 cctagtaaga gggaattaga tgctttaatg tctactggtg aaatgatttc agcatcttta
     3241 ttatcaatag cattaaatgc acttggatgt aaagctataa gttataatgc ttaccagtta
     3301 aatataaaaa cgagtggtct tcatggaaag tctcaaattg atgatataaa cgtaaacaga
     3361 attaaaaata gtttagatga aggaaatgtc gtgatagtaa ctggatttca gggaatcaac
     3421 gaagatggtg atgtaaccac tcttggaaga ggtggttcag atacatctgc tgtagctctt
     3481 gcggttaaat taaatggaaa atgtgagata tatacagatg ttgatggaat ttattttaca
     3541 gacccaagaa aatattcaaa ggctagtaaa cttgatgaga ttgaatatga agaaatgctt
     3601 gagttagcga gtttaggagc acaagtaatg cattcaagaa gtatagagct tgcacaaaaa
     3661 tatggtgttg aaatctatgt cggacgtaca tgtggtacag aaaaaggaac atatataaga
     3721 gggggaaaag acatgaaatt agaggataaa gtaataacag gattagctac tagtgatgat
     3781 gacagttcta taacaataaa agattttaaa gctgaaaata tatctagctt atttgaagat
     3841 atagcaacta taggtataag tgtagatatg ataagccaaa ctgcacctat tttagataag
     3901 ataagtgtat catttacagt tccaaaagaa gagttgggag aatgtaaaaa gatagtatct
     3961 aaatatacag atgaagaaca tgtagtgatt gataataata tcactaaatt ttctttagta
     4021 ggtcttggta tgaaaaatac ttctggagtt gctgctaaag tatttaagat attcaatgaa
     4081 aatggtataa tgataaaact tataactaca tcagagattc gtataacttg tgctataaat
     4141 tctgatgata agcaagtagc tatagaaaaa atagcagaag tatttaatat ataaaatttg
     4201 gggaggctat tatgaaactt catggaacta tgactgtaaa taatgatgaa ttatatattg
     4261 gaggagtgag ttgtttagaa cttagtaaaa aatatcaaac tccactttat gtatttgatg
     4321 aagctttagt tagacaaaat tgtagagaat atgtagaata ttttaaggct agagagggaa
     4381 aaaataaggt tgcttatgca gggaaagctt ttttacctct ttacatgtgt aatcttataa
     4441 atgaagaaca aatgtgttta gatgtagttt caggaggcga gttatataca gcttataaat
     4501 caaattttcc tatggaaaaa gttttctttc atggaaacaa taagactaaa gaagaaataa
     4561 acatgggatt agatttaggt gtaggtaaat ttgtggtgga taatttttat gaattagatt
     4621 tgcttgaagc attatgtgaa gataaaggta aaactcaaga gatatacttt agaataactc
     4681 ctggtataga agctcataca catgattata taaaaactgg acagatagat tctaaatttg
     4741 gatttgcttt agtaaatgga gatttattta gagcaataga aaaattaagt gaatataaaa
     4801 atattaaact tgtgggtctt cacgctcata taggttcaca aatatttgat gtagaaccat
     4861 acatagatac agtggatata atgatgaact tggtaaaaga aataaaagaa aaattcaaca
     4921 tacaactaac agaaattgac ctaggtggag gagttggcgt ttattatagc gaaggagata
     4981 aacctaaaac tataaaagag ttttgtgaaa ccataatcac taaagcagaa acttcatgta
     5041 aagagcttgg aatagaagta cctacattgg taatagaacc aggaaggtca atagttgcaa
     5101 atgcaggaag tacattgtat acagtaggtt ctataaaaga tataaaagat gttagggttt
     5161 atgtaagtgt agatggaggt atgacagata atgtaagacc ttcgttgtac caagctagtt
     5221 atgagtgtag tattgtgaat aaaattaatc atgaggctat gaatcatgta acaatagctg
     5281 gaaaatgttg tgagagtgga gatatattaa ttggagatat aaacataatg gatattaaaa
     5341 gtggagatat acttataaca acatcaactg gtgcatatgg atattcaatg tcttcaaatt
     5401 ataataaaat accaagagca gcagtagtat cagtatttga tggaaatgat aagataattt
     5461 gtaagagaca aagctatgag gacttattag ctttagagat ataaaatgga gaagtatata
     5521 ataaaaatta aatattaaag tttattatga gcagatttgc taggatatgt atctctaaat
     5581 taatttttgg tttattgtat cgttcgtagc atatctgctt attttttgtt ttactaacaa
     5641 atttcttgta ataaatataa aatattttaa aaactttata attatgataa aatttaaagt
     5701 atatgacaag gagggattta atgagaggaa gagtaatttt attaatatcg atattatcta
     5761 tatttcttgt aggatgttca tttaataaaa aagatgaaat aaatttagta gagcaaggaa
     5821 aaacatattt agaaaaacat aactataaaa aagctatgga atcattatcc tctgcactgg
     5881 aagaagatag tactaatgag aatgcaagag ctatgtatat gcaagctatg agaatgtcaa
     5941 atatgacgga gtttgaagaa ttaaaaaatt atgagggtgc aatcaaggaa cttaaactta
     6001 tagagaaaat caaaaatggt tcctctgtta ttaaagaaga ggcaactaaa aagaaagaag
     6061 agttaactaa attagaaaaa gaacaaaacg aagcagagga acaaagaaag aaaaaggcaa
     6121 aagatacggc tggagaagat aggtatagag ttgaatcaga tgcaaggaaa tcttcttata
     6181 gtggaagtag taaaaataat aaatctagtt ctaaaaataa tgataaaaaa tcagatagta
     6241 ataagtctga caatagtaac agttcttctc aaaatcaaga aaagccaaat aatagtaatc
     6301 caacaccagc accaactcca caagatacta ctggtggttc agatagtggt ggaaattctc
     6361 aaaactcaaa taatcaataa gtataaaata tatgcataaa tttaatgaac aaattatata
     6421 ttataaaaaa tatctcttag caattttttc ttactaagag atattttttg agaaataatt
     6481 taataaataa cagttaaatt ttaaactaaa aattaaagtc acagtagaga taagctattg
     6541 taaacgatta tctatatatt tatcaacata agtttttaca acagcagcta ttggtactga
     6601 aagaaaaact cctatgaaac caaatagaga ttgtcccacc aaaatagcag atattaccca
     6661 tatagctcct ataccaactt gattacccat aacttttggt tctataaaat ttccatctaa
     6721 ttgttgcaat ataaatagaa atgcaatgac ccatatagtt ttttgtggac tatataatat
     6781 agtcatggct attggaggga ttgcaccaat aaaaggacca aaatagggaa ttatattacc
     6841 taagaatatt attgaagcta aaaataaagc attttctatt ctgataacaa attgaaatcc
     6901 taaaaatgca attataccaa taataagaga gtctaatatt ctaccaagta aataatcata
     6961 gaaaatatca tgagtagtcc taaaaaattc tataatgtta tctgccttgt ttctagcagt
     7021 agaagaatat agaaatctct taaatcccaa tgcaattttt tctttgtcat atataatgta
     7081 tatagatagt ataaatccca ttgtcatatt aaatagcata gaagatatac taagtgtata
     7141 gattaagaag tttttaggaa ttttaccaac aaaattactt gcttctttta ataaattatc
     7201 taaactatgt tgtatatgag gaattaaagt ttttagaaaa tatatgtttg acatattttt
     7261 ttctataaaa ctatctatat aatcagtata cataggcatt tcatttatca atctatttaa
     7321 tgtattagct attgatggaa ttaataacct aatggcaaag gctaaaataa aacctataaa
     7381 tatataagat aaaaaaatgg ctaaaagtct atgtgctttg aattttatct caaaaagcat
     7441 cacaagtgga tttaaaagta aagcaagtaa aatagctaat aaaaatgggc taaaaaactt
     7501 taagaatcct tcaatactag atataaagtc tgatggagta ttaataaatt taaaaaataa
     7561 aattccaata aacacgacta ttaaataata ttttatattt tcctttgaaa taataatcac
     7621 ttccttaaaa tagtgtttag tcagctatta catattaata gtatgtgaaa agaaattaca
     7681 attatttagg aaaattaaat aatttttttg cttgttttta attaaggtct agtatagtat
     7741 aataatgtag caaaattaac attgataaaa atattaaaca cacataattt aagataagga
     7801 aggattaagt atgagaatta ataagtatat agcttcttgt ggaatagcat caagaagaaa
     7861 agcagaagaa ataattcttg gaggaagaat aaaggtaaat ggaagtgtag taaaagagtt
     7921 atcttttaat attgatgaag aaaaagacat agtagaattt gataataaaa aagtaaaacc
     7981 aagtgaaaat tatatatata tagtattaaa taaaccagag ggatatataa ctacagtaaa
     8041 agaccaattc aatagaccca gtgtaataga catacttaaa gatgtaaggg aaagagtata
     8101 tccaataggg agattagatt atgaaactag tggtttatta atacttacta atgatggcga
     8161 tttaacatat aagcttaccc atccaaagca tgaaattgat aaaatgtatg tggcaagtgt
     8221 gaagggaatt ataagtgatg atgagataag acaattcgaa acaggactaa aaatagaaga
     8281 ttacacaacg gcacctgcca aaataagggt aactaaagaa aataaagaaa aaaattattc
     8341 agtgtgtgaa ataactatac atgagggtag aaatagacaa gtaagaaaaa tgtgcaaagc
     8401 tataaatcat ccagttttaa atttaagaag gatttctgtg gggaaaatag tattaaaaga
     8461 caccaaagtt ggagaatata gatacctaac agaagatgaa ataaaatatt taaaatcaat
     8521 aaaataagat aaaaataaat tgagaaaaaa tgaaataaag ttaatagtag ggagaaatag
     8581 tattatggca tatgcaaagt atttaactaa aattacagat ataaataaat tttatttaga
     8641 aaatattata aatgaaggtg acatagtaat tgatgctact atgggaaatg ggtatgatac
     8701 aaggtatcta gctgagaagg ttggagaaaa gggctttgta tatgcttttg atatacaaga
     8761 agaagcaata aagtctacaa gaaagaaact agagaaagaa ggatatacag atagagtaaa
     8821 attgatattt gatgggcatg aaaaaatgaa tcaatatata aaagaagaag tttcatgtgt
     8881 tttatttaat ttgggttatc ttccaagagc gaaacattca attataacca aaccagatac
     8941 aactttaaag gctataaagt taagtttaga aatgttaaaa gaaaatggag taataagcat
     9001 tgccatatat acagggcatg atggaggtca agaagaaaaa gattgcatat ataaccttgt
     9061 caataattta aatcaagatg aatttaatgt tttagaatct aagtttttaa atcaagtcaa
     9121 taacccacca caattgattt taattgaaaa gaaaaaagca taataaatac taaaaaactt
     9181 gacaagattg tattaagagt taatatatat agtgtgacct attaaaaata ctttaaataa
     9241 taagtataca acagtttatt ctcaaaacaa aatatacatc taaatatgca aaataagata
     9301 gataactttt aaatgagtaa aaattgaaaa atttaaaaga aatattggat aaatattaaa
     9361 aaatgcaagg aaagatggta taataaattt aatcgaaaat actatgtgaa gtataaggta
     9421 cagaaaagtt agaattttat gagtttaaga agctatgata aaaactaaga tgaaattgag
     9481 ataaaaaata aaatttttag agaaaaagac agtgttaata aaacaaaata gtttatataa
     9541 ctaaaaggag aaaaaaatgg aagaaacaaa tgaaatgaag gacagtaaac atttgatatc
     9601 aaatatacaa tctcaatata cgagattgag taagggtcaa aaacttatag cacaatacat
     9661 tttaaataat tatgataaag ttgcatttat gactgcatgt aaattgggtg aaactgtagg
     9721 agtaagtgag tctacagttg taagatttgc aaatgcatta ggttatagtg gatatcctaa
     9781 attacaagct gcacttcaag aactcataaa aaataaacta actacagtac aaagagttga
     9841 aatggcacat gattattccg atgattttgc aatactaaac aaagttttaa aaagtgatat
     9901 agataatata aggtctacac tagaagaaat tgacgagaga gcgtttaaag aagcttctaa
     9961 caaactttta agagcgagaa aaatatatat acttggaatg aggagttctt ttgtagtagc
    10021 tcaatattta ggtttttact tagatataat acttgacaat gttcatataa ttagaatgga
    10081 tatgggagat gcttttgagc agattgtaag aataaatgaa gaagatgtaa tagttgctat
    10141 tagtttccct cgttattcta agaaatctta tcaaattgtc aattatgcta aagaaaaagg
    10201 agcccatgta atatcattaa cagatagctt atttgcgcca gtagcatcac ttgcagataa
    10261 cactcttcta gttaagagta atatggcttc atttgtagat tctctagttc cagctcttag
    10321 tatttcaaat gctcttgcta tatctgttgg gatgaaggaa aaagaagata ttaaacagca
    10381 ttttgatgat ttagagcaaa tttggaaaag atattctgtg tatgagtaaa gtttgtgtat
    10441 taataaggct atgaaattga gaatcatagc cttattaata aatactagat tatcacttaa
    10501 aaaataaatt ttcgtttaaa taatacaaat aaatatttcg agactcaaaa cataaagatg
    10561 tattctgaat atatctttac tacatcaact aatttattaa agaaagcatt tcccttcatg
    10621 gagagtgctt tttttatttc tctaaacaat atcatttcct taattaacaa ttataaaatt
    10681 aattttattt gtatttaagt atataatatc ttattaaaat tctattaaaa tatgtatttc
    10741 tatctttttt gttcactata atatgtgtct tttagtattt tattttaact ttgacaaaga
    10801 agttgaaaaa tttaaaaatc atagaattag tagttaggta tgaaatcttg attttttaag
    10861 tagccatttt tggggaaagg gaaacttttt tagaaatata atactttata aaa
//
LOCUS       DAEMGG010000128        10904 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEMGG010000128 DAEMGG010000000
VERSION     DAEMGG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.129"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            294..1454
                     /locus_tag="KPK24_003133"
     CDS             294..1454
                     /locus_tag="KPK24_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG2300782.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1797..1958
                     /locus_tag="KPK24_003134"
     CDS             1797..1958
                     /locus_tag="KPK24_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300783.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2151..2780
                     /locus_tag="KPK24_003135"
     CDS             2151..2780
                     /locus_tag="KPK24_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG2300784.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            3020..4279
                     /locus_tag="KPK24_003136"
     CDS             3020..4279
                     /locus_tag="KPK24_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG2300785.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4538..5533
                     /locus_tag="KPK24_003137"
     CDS             4538..5533
                     /locus_tag="KPK24_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG2300786.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5779..6846
                     /locus_tag="KPK24_003138"
     CDS             5779..6846
                     /locus_tag="KPK24_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG2300787.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYNKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7380..9290
                     /locus_tag="KPK24_003139"
     CDS             7380..9290
                     /locus_tag="KPK24_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG2300788.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9353..9976)
                     /gene="ytaF"
                     /locus_tag="KPK24_003140"
     CDS             complement(9353..9976)
                     /gene="ytaF"
                     /locus_tag="KPK24_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG2300789.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10164..10808
                     /locus_tag="KPK24_003141"
     CDS             10164..10808
                     /locus_tag="KPK24_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300790.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         4484 a         1066 c         1770 g         3584 t
ORIGIN      
        1 aataaataca aaacaaaata aataaataat aaaatattta gtttaattat ctttacaaat
       61 aaaaatttta gtaataaaat aattgttgaa aaagatttta aattcagatt attcatttct
      121 aaagtaatta atgtgcaaat ctttattaat atatgtcaat agttaggtat atatgtattt
      181 aattagcatg atatagataa aagtatttat tttaattgtc tattatctga aaaagtcttt
      241 acttagaaat cataaaaatc aaattaatta ggaaaagaaa gggtgtgtat aatgtgattg
      301 agactggatt tgtatattgt agtttcttgg ttgtattcgt aggtgtaatt ttttttatgg
      361 ttgataaatt gaaatggaag gtatttgatg ttattaatcc aatgctttta atatacttag
      421 gttcagctat acttggatca tttggtttat ttgcacagaa tgaagatgtt ggaacgtatc
      481 aaaacataat aacagtgaac ttccttccat taatgttagt gtttttgtta attcaatgtg
      541 atttaagaaa aatattaaag atggggccta aaatgttatt atcatttttc tgtgctacat
      601 ttacatttat gcttggattt gttatagcat tcataatttt taaaaattca atgggtgcag
      661 attcatggaa aattcttggg gcagcatctg gttcatggat aggtggctca tcaaatatga
      721 ttgcagcagc gaatgctcta ggagttgcag aagaaggact tagttatgca atacttatgg
      781 gttctatagg atatacagta tggatgtctg tttgtctaat gtctgtgaaa tttggaccta
      841 aatttaacaa atggacaaag tgcgatacaa gttttataga tgaggttaca tcaaaacttg
      901 aaaaagaaaa taaagataat acagcaccta cgtttgtaga acttatgatt ttactttctg
      961 ttgggtttgg agttgcgtct tttgcacaaa ttgtatccaa gatacttcca acttcaggta
     1021 tacttaatga aactatgtgg gtagtacttg ttgcatctac agtatcaata ttattgggta
     1081 tgacaaaaat atcaaagctt aaagggagta cactggtagg aaatgtgttt atgtatttac
     1141 tccttgcagc tacaggtagt aaagcaaatt tctttggatt gtcagaagca cctgtttaca
     1201 taatgtttgg attggtagta gtccttgtac atgctatttc atttataggg cttgctaaac
     1261 tatttaaact tgatttattt acttgtgaag taggaagtat agctaatata ggaggagtaa
     1321 ctacagctcc aatgattgct ggggttcaca atcctgtatt aataccagta ggtgtactta
     1381 tggggcttct aggaaatatt atagggactt attgtgctct tatggtaact caaatattac
     1441 atatgttagc ttaataaaaa tatggtataa aattaataat ttaaataaga aaagtcattt
     1501 caatttaagc agaagctttt actgaaatgg ctttttttgt ttgacaagat aatctatttc
     1561 aagaagatgt tttgacatat ttagtgtata atattgtaaa atttactaaa aacgagatat
     1621 atttaatttt tatattgaat cgaacgtctg tatgatgtat aataaatata cagaacgaat
     1681 gtttgcagaa cgggtgtttt ttgaatttat tttaataatt tatgaatata tatcagaatg
     1741 cataaatata tttaaatata aagattaaaa agaatttaaa ggagtgttga taacttatga
     1801 taattccatc aagaagaata caagatataa cattaacaaa tttaaaaaat ggtgaagtaa
     1861 ctttaattga attggatgaa atatacaaga agatgggatt tttatttgtt gttagtgaag
     1921 gcaaattgaa aaagataaaa aaggagaatc atcattagaa atgaatgaaa taataaatat
     1981 tataatagtt tataaaacct aacttatgta gaatataaat acttaagtta ggtttttttg
     2041 tgataaagtt tataagtact caattttata agataaataa tggttttaag tgaaaagtaa
     2101 ataagtttaa attttagtaa gtattatatt gcataagagg tgatattatt atgaaaatag
     2161 taacctacaa tattcataaa ggtatggatt caaataatag attaactcta actaaaatgg
     2221 ggttatattt aaaacaattg gattgtgatg taatttgttt gcaagaagta ttatatcctc
     2281 aatttttagc tttaaaagct gttttaaata tggatggtgt ctttgcgaca aatgtaaaaa
     2341 aagtaaatat gatatatggt atttgtacat ttacaacatc taaaatgtta aacagtaatc
     2401 attttttctt aactagcaaa aaagaacaac ggggagctct ttgtataact atagatgcat
     2461 atggaagaat tataaatgta ataaatactc acttgggact tgataggcag gagagagcaa
     2521 aacaattaga cgaaataata gactatagga acagattagt ggggatagtt attttatgtg
     2581 gtgattttaa tgagaaaaat gtctttttaa gtatgtttaa tgatatggct atttcactaa
     2641 acaagtctta cttaccaaca tttgaaaaat caaactctag aatagattac atttttgtta
     2701 ataaaaatac tgaactaaaa ggatacacag tagaaaaaat ttacctgtca gaccattatc
     2761 ctgtaatagg ttacatttaa atttcaatat aaatgaaatt agttgttata aaataaacaa
     2821 tttttttgtt aagaaatagt aaaagaaata taataaaaaa tatcaaaaat aaaactaatt
     2881 ataagacata aatttacaat aaagttacat ttgattaaat tccttgttta acaaaagcta
     2941 ttcaaatata atctaaggta gttttgaaat gggaactcta ttatgcaaat ttaagctagt
     3001 gttaaaatga ggtgaagtta tgggcagaag aacaacaaga gtttcaagat taggtaaaga
     3061 aaaaaaacga agtgttttaa ttgtattggc agctttagtt ctaataatgg gtggatttac
     3121 ttattttttt aacagtaaat ttctgtacaa tgggaaaatc gctaaaaatg tatatataga
     3181 aggagtaaat gtatctgata tgactaaagc agaggcatta aaagctataa cagataagta
     3241 tactccagaa gatttaaatc ttgcttatga tgggaaaaaa tatacaatat caccaaaaga
     3301 tattgattta aaatatgata cagaagatgt tgtaaaagat gcttatgaaa gtacaaaaaa
     3361 agggtcctat tttcaaaact taaaaaaata tattgatata agagtgaata aggcaaatat
     3421 gaagataaaa gctgaatatg atgaggcaaa acttagttcc aaagtgtctt ctatagcaga
     3481 tagtataaat gttaagatga aaaatgcttc tattagtgtt ggaagtggtg gactaagtta
     3541 tacagactct gttgtaggta gagaatttga tttggcagca aataaagagt ctatatataa
     3601 tatgataaaa aataaagagc ataaaacttt agaacttaaa gtaaatcttc aaaaaccaga
     3661 tataacaact gaacaagtaa aaactgtaaa ttctgttata ggtcaatatt caactactta
     3721 ttcacaagct gtagagggta ggtcatataa tgtaggatta tcagcaagaa aaactagtga
     3781 tgtattatta atgccaggtg aagaattttc atacaataaa cttacaggac ctagtaataa
     3841 agcaaatggt tacaaagatg cacctgttat agtttatggt aagctagaac aatcagcagg
     3901 aggaggtgtt tgtcaaacat cttcaacagt ttataatgcg gctcttcttt cgggtatgga
     3961 gataactcaa gttacaaatc attcatctgc atctacttat gtgcctaaag ggagagatgc
     4021 tactgtgagc gatggaggat taaacttaaa gtttaaaaat ccatataagc atcctgtata
     4081 tattaaaaat tatgcaggtg gaggaagtgt atcatcagta atctatggta attctggtga
     4141 caaaccaaat atatctatag aggtaaaaca aactggtcag aataaatatt ctacatatcg
     4201 aatatttaaa gattcaagtg gaaaggtcat aaaaaaagaa catatatcaa atagttctta
     4261 taaagaatta aaaaaataga taatttttta gagaaatatt tgtaatataa aagggagtgt
     4321 ctcaaaatga actttttagt ttatgaggca ctctttttct gtgaaatcaa atatattttc
     4381 atcatttcaa caatacaaaa gaagcgtatc tctattttga gacagccttt tttgtaaaaa
     4441 ttaacactat tagttcttgt tttaatatta caatcattaa tatatagaaa atattcctat
     4501 atattggtat aattgtaaat agatttagtt atataaaatg tatgaggagg tgacatggtt
     4561 gaattttata ggcaatagat atgaggtagt aaattctgat gacgttttag aaattaacaa
     4621 aatctataaa gctagagatg ttttttacag gaaaaatgtg ttaataaaag ttataaagca
     4681 caataattat atctgtgaag attttgtttc aaatttaatt gatgaaagta ctgctttaga
     4741 tgagataaac tctccttata tactaaaaat aatagatgtt gggattcact gtacagaaga
     4801 aacaacatta tattatgttg taagtgaaga ctttaatggt atcggactag atgaattaat
     4861 tctaggtaat tatcttcatt tagaagcaat tgtaaacata atgatacaag ttttaaaagc
     4921 tttagaaatg atacatagga atcattcata tcatggtagt ttaaaatcga gtagtataat
     4981 agtagataca gaatacaata taaaaatttg tgattttgga attacaaaag caaataatgg
     5041 ggttaataca agaagctatg gaaacaggag atatttatgc ccacatcaac tatgtataaa
     5101 ttatactgat aaagaaagtg atttttttgc aacaggtctt attctatttg aatctatatt
     5161 taaaaaattg ccttttggag aatcaaattc agaagaaaaa atgctacaat ctattgataa
     5221 gggattagat tggaatagta taaaagcaat taatggaaat actgaactta taaatgttat
     5281 aaaaaggctt cttggaagaa ataataagta tactagagcc aatgatatta ttattgattt
     5341 aagtaaagta atgtatgaaa aagcatatat agaagttgaa gatgagaaag aagaagagca
     5401 gaaacaagac aaagttatac aagttaaaaa agaatataaa agtaaaatga gaaaaaaatt
     5461 acataaaaaa cttgttgttg ccggtctagc tatagtcatg atttcaatga ttattattag
     5521 ttccataata taaaaattgt atattaagta ttttaaaatc aatgtctata aaggatatac
     5581 atagaaattt tagtgtagtc ttaaattatg aaaggaagtg gttattatgt taacaaaaaa
     5641 taatgaggta ttaaacgtct aggagtattt taacaaatag ccacctgctt ttacagttta
     5701 gaataaaaaa gctgtggcta aaggtcacag ctttttgcta ctttttaaag gcaggagaat
     5761 attaaaaagg agcaaattat gagcaatata aaaataatag caagtaataa atgttggatt
     5821 gaagatatag caaggaaaca acttgaagat atttcaaagt taaatggaat tttaagagta
     5881 gtaggattac ctgacttaca tgcaggtaaa atacctgtag gtttagcagt tgagactaaa
     5941 aatattattt atccacatat aattggaaat gatataggat gcggaatgac tttatttaaa
     6001 actggggttc taaaaaagaa atttaaaaaa gacaaatgga ttaagtcact tagtaagatt
     6061 aaagatttgt ccgatataga aataaaaaac acatataaag aagaatgtcc aattttaaat
     6121 ttaggaacta tagggagtgg aaatcatttt gtagaaattc aatgtataag tgaaatttat
     6181 aataaagaac aatttgaaca attaaaattt tcatctgatg atattatgat gttagttcac
     6241 tgtgggtcaa gaaattatgg agaagatatt ttaaaaaaat tttatgataa agatggactt
     6301 gaagttgaaa gtgagaaagc tatagattat attaaaaaac atgataatgc tttaatgtgg
     6361 gcagagcgta atcgtgaagc tatcagtaaa aaaattatgc agtcaatagg tacatctgga
     6421 gatgttgaaa caatattctc tataaatcat aattttatag agaaaaggga agataaattt
     6481 atacatagaa aaggtgctgt ttcaagtgaa agaggagctg tgattatacc aggttcaaga
     6541 ggcagtctat catatatagt tatgcctact gaaaatacag agatatcttt atattctcta
     6601 tcacatggag caggtagaaa atggtctcgt tcagtttgta aatctcgcct taaaagtaag
     6661 tataataaag atactataaa acaaactaaa tttaaaagtc agattatatg taatgatgta
     6721 aatttattat ttcaagaagc tccagaggca tataagaata tagagcagat tattgaaagt
     6781 ttgatagaat atgaacttat tcaagtagtg gcaactatga agccattgat tacttataaa
     6841 ggataatttt taaagttaaa aaagattaaa attatattat aaatagctaa ttttatggaa
     6901 catttaaaag tgttttatta ttagctattt ttttattttg tagagacgat aatttataga
     6961 gctaaattct ttgatatata atgattacaa agttagactg tattggaaaa tgcacataca
     7021 tgaattgatg acaatataag tgattcccaa atataaatga aagtataagc ttattttcac
     7081 tataaaaatt ttcactgtac aaaaacatta atgacattat ttttattgac ataaatatat
     7141 aatttatgga ataatattag aagcactgat ataagtgttt aaataagaat agaaaaggag
     7201 aagtgtttta tgaaaaaaaa tactaacatt aatgagtccg gagttaaact tcttaataaa
     7261 taaggagttt gatatataat aactccttat ttattaagtt gttcgcaaaa taactattat
     7321 caaagtaaat gatagggtga tttaaaagac gataacttat aaataaagga gaaaaaataa
     7381 tgaatagtaa tacatttgaa agattacaac taaatgaagt taaagaatta ataaaaatac
     7441 attgtgtaag ttcactagga aaaaatttaa tagataaatt aactcctagt gggaatattg
     7501 gagtagtaag aaggaaatta gcagaaaata aagaagctag aaagataatt gaaaatagta
     7561 atcacatacc acttgaagga ttgtttaatg caggttctac aatagataaa attgaaaaag
     7621 gaatgataat agaacctgtt gaacttgtaa atattgaaga ttttttaaga ggttgtagga
     7681 agatgaaagc ttttatgtta gaaaaagaat tttattcacc tacattaagt tcttatgcct
     7741 taaatataac agaatgtaaa agtatagaag atgagataaa ttactgtata aaatctaata
     7801 aagttgattc taatgctagt aaagaattaa aaaagataag acgaaatata gaaattacag
     7861 aaggaaagat aaaagataga ttaaataaat ttataacatc aactgtaaat aaaaagtata
     7921 tacaagagtt tataataagt aaaagaaatg atagatatgt aataccaatt aaatcttctt
     7981 ataaaaatga agttaatggt actatattag atacatcatc aaaaggaaat acggtattta
     8041 ttgaacctat tagtgtttca aatttaagta cagagttaac tatgttaaaa gctgatgaaa
     8101 ctattgaaga atacaaaata ttatcttatt taacagaact tatatttgag aagataagtc
     8161 agataaaatt aaatatagag atactttcag aatacgatat ggtatttgca aaagctaagt
     8221 atagtcaaaa aataaaagga attactccaa aaataaataa taatggctat ataaaaataa
     8281 ttaaaggtaa acatccactt ctaacaggag atgcagtacc tttagatttt gaaataggca
     8341 aaaattatag aagtcttata ataacaggtc ctaatgcagg aggaaaaact gtaaccctta
     8401 agactgttgg tttactaact ttaatggttc aatgtggatt tgatatttct gcaaaagaag
     8461 gtagtgaatt tagtgtattt gaaaaagttt ttgtagatat aggggataat caaagtattg
     8521 aaaatgcctt aagtactttt tcatcacata taaaaaatat tgcagagatt atgagtttat
     8581 caaataattc tactcttgtt ctgtttgatg aaataggaag tggaactgaa cctaatgaag
     8641 gagctggttt agcaatatca ttattagaag agttttacaa aatgggatgt ataactatag
     8701 catcaactca ttatggagag ataaaaaagt ttgctacatt gcatccagaa tttgaaaatg
     8761 ctggtatgat gtttgataaa gaaacgttag aaccattgta taagttaact attggaaaat
     8821 ctgaagatag taatgctctt tttatttcta aaaaaatggg aataaaaaat agagttttag
     8881 atagagctaa agaatatgta attgatagaa actacaattt agatttagtt aaaagaagta
     8941 agctacaatc taatattgaa gataaaaata gttctgaagt cattttaaac tatacagatt
     9001 ataatgtagg ggataaggta aaattactag atgaagatga ttttggaatt gtatataagc
     9061 ctatggacaa gtttaataat gtagaagtat tgtttaatga taattttatt tcagtaaata
     9121 taaaaagatt acaacttagc ataaaagcat cagaattgta tccagaaggg tatgatttag
     9181 atgtattatt tactagtttt aaagaaagaa aattagaaaa agatattcaa agaggttcaa
     9241 aaaaagctct taaaaagatt cagaaagaaa ttagagagaa tagaaaataa atataaaaag
     9301 cacaaaagaa agcaatctaa agagaaaagt ttatctttag attgctttct tattaacata
     9361 tcttcataaa tccaatgact aacaagatta aaccacttaa ccaagataag tttaaattta
     9421 cgtttttaac aaacatattt cctagtaaac aaccaagtaa tactgcaatt atatttgata
     9481 ttaaagaaaa tagaataact tgtatataat tcacacctac aaggccacta ccaaaaccga
     9541 tagctaatcc atctaaactt aaagcaagtg caagtgtaaa tgcttcagca gatttaagca
     9601 ctttagattt acttgtatct aagacatcag gttcaatact tacattaaaa tttaatttaa
     9661 ggtctaacat attaaaatta taattctttg agtgtataga ttttttctta aaataagatt
     9721 taaaaaaact ttcaattagt ctagcacttc ctaataaaaa taaaataaag aaacatatta
     9781 acattgttat attttgaggt aaaaagtctc taactattgt acctgcaaaa agcgatattg
     9841 ctaaaatagc tgaacaaact acgctgataa ttatatttga atataaggga actttaactt
     9901 tatttacgcc atatccaaag cttgtcacaa aggcatctat acaaagagaa gtaactaata
     9961 aaattgattc aatcacagta gattaacatc ctttctctta aattttgtac ttatattagt
    10021 tatagtattg taaaaatgga taaattgtta cagataatcc aataaattgt tgataaaatt
    10081 ccaacatcat acaacaccta aataacaaaa gaatcatatc atataataat ataaaaccaa
    10141 tcataataaa gcaggtgaaa ataatggaac aacatagtaa ggttgggaat ttcggtgatt
    10201 tgttgaaagt tttattagat gaaaaagaat tatcaatggg agaactaagt aaaaaaagtg
    10261 gaattgataa atctactata tctcgtatag ctaataataa acaaaagcca aatataaatc
    10321 atttagaaaa gatggcaata catttaaata ttaacctaga agaactgttg aaagcttcag
    10381 gatatgaatt tagaaacagc aataatcaaa tatttaatgc agatagtgat tttagtaact
    10441 ttgatgatat tcttggattt gcaaatttaa tcaatgataa gaactttaat ggaaatattg
    10501 aaaaagagtt aagtaagtgt aagttatatg tccaaacaga tgaaggtaaa aaattacttt
    10561 ttgataattt taataaaaaa atagattcta ttgaaaaaca aggtcaattt acagatagat
    10621 taagacaaat gtatgctgat ttttgtacag atggacttac aataaagaaa tatttattga
    10681 ttggaagtat gctactttac tttgttatat caacagatgt gattccagac ttcgtgttcc
    10741 caataggatt tatggatgat ttagtagcat taaatatagt tacaaaatta cttaaaaatg
    10801 ataagtaagt tttagaatca tatttaatat ataaataaat caaagtatat aaaatttatt
    10861 attttttata tgaaacaaat taaaagcaca atgaagtaga ttaa
//
LOCUS       DAEMGG010000129        10885 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEMGG010000129 DAEMGG010000000
VERSION     DAEMGG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.130"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..2137
                     /gene="smc"
                     /locus_tag="KPK24_003142"
     CDS             <1..2137
                     /gene="smc"
                     /locus_tag="KPK24_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG2300791.1"
                     /translation="KLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNRGVKEVLKNK
                     NLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYLKQNNLGRVT
                     FLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTILINNIDEGI
                     KFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTEKISNIKNEI
                     SHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEIESLKGSITK
                     LENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKRYNDLYDKEK
                     SEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEEHEIIKLQDS
                     ILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQHIELKESLF
                     KVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDENLDIDRKFLESLKREIRSLGN
                     INIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFEIKFEEISKN
                     FKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGGEKALTAISI
                     LFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHRRGTMEAADY
                     IYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            2164..3444
                     /gene="ftsY"
                     /locus_tag="KPK24_003143"
     CDS             2164..3444
                     /gene="ftsY"
                     /locus_tag="KPK24_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG2300792.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            3547..3915
                     /locus_tag="KPK24_003144"
     CDS             3547..3915
                     /locus_tag="KPK24_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG2300793.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            3912..5264
                     /gene="ffh"
                     /locus_tag="KPK24_003145"
     CDS             3912..5264
                     /gene="ffh"
                     /locus_tag="KPK24_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG2300794.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            5302..5574
                     /gene="rpsP"
                     /locus_tag="KPK24_003146"
     CDS             5302..5574
                     /gene="rpsP"
                     /locus_tag="KPK24_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG2300795.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            5639..5866
                     /locus_tag="KPK24_003147"
     CDS             5639..5866
                     /locus_tag="KPK24_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG2300796.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            5974..6489
                     /gene="rimM"
                     /locus_tag="KPK24_003148"
     CDS             5974..6489
                     /gene="rimM"
                     /locus_tag="KPK24_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG2300797.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            6498..7193
                     /gene="trmD"
                     /locus_tag="KPK24_003149"
     CDS             6498..7193
                     /gene="trmD"
                     /locus_tag="KPK24_003149"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG2300798.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            7378..7728
                     /gene="rplS"
                     /locus_tag="KPK24_003150"
     CDS             7378..7728
                     /gene="rplS"
                     /locus_tag="KPK24_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG2300799.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            8049..8945
                     /gene="ylqF"
                     /locus_tag="KPK24_003151"
     CDS             8049..8945
                     /gene="ylqF"
                     /locus_tag="KPK24_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG2300800.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDAETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            9334..10626
                     /gene="brnQ"
                     /locus_tag="KPK24_003152"
     CDS             9334..10626
                     /gene="brnQ"
                     /locus_tag="KPK24_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG2300801.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         4575 a         1079 c         2058 g         3173 t
ORIGIN      
        1 taaactagaa gatgaaatac agaaaagtaa atataattta aatggatata attcaaagtt
       61 aaatgtctat atagatatgg aaaatcatta tgaaggattt aatagaggag taaaagaagt
      121 actaaaaaat aaaaatttaa aaggagttca tggagcatta ggacagatta taaatgtacc
      181 tgaaaagtat gagaaatcta tagaagcagc tcttggtgct tatatgcaaa atataataac
      241 tgataatgag tttagtgcta agtctgcaat aaactatcta aaacagaata atttaggtag
      301 agtaacattt cttccattaa atataattaa atcaaataaa ataagtctag gaaatttaaa
      361 agcaaatact aagttcatag gaatagcaag tgatttaatt acatttgatg aaaagtatag
      421 aaatataatt gaaaatatac ttggaagaac aatacttata aataatatag atgagggcat
      481 aaaatttgca aaagaaactg gtcataggtt taaaatagta actttagatg gtgaaatctt
      541 aaatccagga ggttctttga ctgggggaag tctaaaaact aatgggaata tactatcgag
      601 aaaaagatac attaatgaat atacagaaaa gataagtaat atcaagaatg aaatttcaca
      661 tctagaatta aaaagagaat ctttagacaa agatgtgaaa aatatcaaaa atgaaattga
      721 ttcacatgaa agtaaaataa aagatttaga aaaaagtata ataataaaaa gtacaagtat
      781 aaagaatgtt gagtctgaaa ttgaatcgtt aaaaggtagt attactaaat tagaaaatga
      841 gaagaatgat ttaaattcta acctaaatta tacacttgaa aaaagtgatg atgttagaaa
      901 agatatggaa gagttagatg atttatacaa taaaaataaa gaaaaaattg atgcattgaa
      961 tgaggaaata aaaagataca atgatttata tgataaagaa aaatctgaat ttgatgaatt
     1021 gaacttaagt ttagtgaaga aaacagaagt ttacaatagt atagttagag atataaaaag
     1081 aatatctggt gaaaattgtg agctagaaga aaaaaataaa caattagaag agtcattaaa
     1141 ttatgaagaa catgaaataa ttaaattaca agattctata ctcactgaag aaaaagaaaa
     1201 agaaaattta acaaaacaac taggagatag taatagaaat cttgaaacta gaaaaatagc
     1261 aaaagatgat ttaaaaaata gttttgatga aattaataaa gaattaaaaa ctatagatag
     1321 acagcatata gaacttaaag aaagtttatt taaagttggt ggaagattag aaagattaaa
     1381 aactagtcaa gacacatata taaataagtt gtttgagcaa tatgatatga ccttagtaca
     1441 agcattggaa attaaagatg aaaatttgga tatagataga aaattcttag aaagtctaaa
     1501 gagagaaata agaagtcttg gaaatataaa tatagactct ataaaggaat atgaggaaat
     1561 aaaagaaaga tacgattttt atagcgaaca aaagcaagat ttagaagagt ctatggaaga
     1621 gatagaaaaa cttatacata ctttagaaga aaatatgaaa tctgaatttg aaattaagtt
     1681 tgaagaaata agtaaaaatt ttaaatatgt atataagaga ttatttggtg gaggttgtgg
     1741 agaattaact attttagata aagaaaattt attagaaagt gatatactaa taacagctca
     1801 acccccaggt aaaaaaatga aaaatttaaa cctattatct ggtggagaga aagctttaac
     1861 ggcaataagt atattatttg ctatattgat tacaaagcca acaccatttt gtatattaga
     1921 tgagattgaa gcgccacttg atgacgcaaa tatatttaga tttggagaat ttttgaagga
     1981 tttatctaaa gagactcaat ttatatcagt aactcatagg agaggaacta tggaagctgc
     2041 tgattatatc tatggagtta caatgcaaga aaaagccatt tcaaaagtta ttagtttaaa
     2101 attaaaagaa gctcaggaaa taacagatat tatataactt agattgacat aggaggaata
     2161 acagtgttaa aaaaattatt tggttttgga aaagacaagg aaaaggaaat agaaaaaaaa
     2221 gatgctgaag aagaaataga agttgaagac agtgttgata atttagaaaa cttggaagaa
     2281 actatttttt caggacttga agaagaagtg atagataaag tggaagatgt tgaagaaaaa
     2341 aatgaggaaa gtgtcaataa tgaaggcact gaagaaatcg aaaactttga aaaaatagaa
     2401 gttgagatgg atagtaatgg aaaagaagta gaaaatattt caaataatga ttctcaagaa
     2461 gagataatcg aagaattaga aaattatgat gaaattgagt ctaaagagat agaagataaa
     2521 gaagataaga aagttaactt gtttgaaaga ctaaaacaag gattgacaaa agcaaagcaa
     2581 ggaataactg atagaataga tgaagtttta aaatcatata caaagataga tgaagaatta
     2641 cttgaagatt tagaggaaat tttaataaca gcagatgttg gtgtaaatac aactatggat
     2701 ataattgaaa gactaagaga taaaataaag caaaaaggta ttactgaacc aataaaggta
     2761 agagaagaac ttaaatctat agttgaagat attcttacaa atgaaaactc tacactagat
     2821 atagaaccag caccatgtat aatattaatg gtgggtgtaa atggggtcgg taagactact
     2881 acaataggta aactagcaaa tagatacaaa aaagatggta aaaaagtttt attagcagca
     2941 gcagatacat ttagagcagc agcgacagag caattagaaa tttgggcaaa tagaaccaat
     3001 gtagacataa taaaacatca agagggagca gaccctggag ctgtagtatt tgatgctata
     3061 aaagctgcaa aagctagaaa aacagatgta ttaatatgtg atacagcagg aagattacac
     3121 aataaggcta atcttatgaa tgaacttgga aaagtattta aaatagtaga tagagaattt
     3181 ccagaagcaa aacgtgaagt acttcttgtg gtggatgcta caacaggtca aaatgcagtt
     3241 gttcaagcaa agacatttaa agaagttgca gatataacag gtatagtact tacaaaactt
     3301 gatggaactg caaaaggtgg agtagtcctt gcagtaaaat cagaagtaga tgtacctgta
     3361 aaacttattg gagttggaga aagtgtagaa gacctacaag atttcaatgc aaaatcattt
     3421 tcagatgcat tatttggtaa ctaatgttct tttaaaaata atggttgaca agagtgttgt
     3481 tatgatataa aatacaatag tgtaaagtaa ataaccttta cagtatactg ctaggaagtg
     3541 taagatatga atatagaaaa aatggtcgaa attggattgt tatttgaaca gtataaagaa
     3601 ttattaactg acaagcagaa agaaatagtt gctctatact atgaagaaga ttattcttta
     3661 ggtgaaatta gtgaaaatct gaatgtatca agacaaggtg tttatgatac cttaaagcgt
     3721 tcagaaaaaa tactaagaga ttatgaagaa aagttacact tagtatcaaa gattcaagag
     3781 caggaaaaaa atattaagat tataaaagat aaaattattg acattaaaga agatttattg
     3841 cacaatagag attgtgctaa tttaatccct aagctagaaa atatagaaga tgtatgtagg
     3901 gagatgataa aatgatattt gaaggattgt ctgataaact acaaggggca tttagtaagt
     3961 taaaatctaa aggaaaactt acagaagtag atgtgaaaaa tgctatgaga gaagttaaac
     4021 ttgctcttct tgaggcagat gttaacttta aagtagttaa agatttcata aaaaaagttc
     4081 aagagagatg tgttgggcaa gaagttatgc aaagcttaac tccagctcag catgtaatca
     4141 aaattgtaaa tgaagagctt acaagtttaa tgggagatgt acaaagtaaa gtaatgattt
     4201 cctcaaagcc tcctacaatt ttaatgatgg ttggattgca aggtgcaggt aagactacta
     4261 cagcaggaaa acttggagga tattttaaaa agcaaggtaa aaaacctcta ttagtagctt
     4321 gtgatattta tagaccagca gctataaagc aacttcaagt tgttggagaa aaattggata
     4381 tacctgtatt caatatggga gataaagaaa atccagtaaa tattgcgaaa gcaggtttaa
     4441 gccatgcgat taaaaatgct aatgatttag ttattataga tacagctggt agacttcata
     4501 tagatgaagt acttatggat gagttaaagt ctattaaatc agaagttaag ccacatgaga
     4561 tacttttggt tgtagattct atgacaggtc aagatgctgt aaatgttgca gaaagcttca
     4621 atgaggcact tggtgttgat ggagtagtat taactaaatt ggatggagat actagaggtg
     4681 gtgctgcact ttcaataaga gcagttacac aaaagcctat aaaatttatg gggatgggag
     4741 aaaagctaga tgatatagaa cctttccacc cagatagaat ggcatcaaga atattaggta
     4801 tgggagatgt cttaagttta atagaaaaag cacaagaaaa tatagactta gagaaagcta
     4861 aagaattaga gcaaaaaatt aaaaaacaag agcttgattt tgaagatttc ttacaacaaa
     4921 tggaacaaat acaaaatatg ggacctcttg acaagatact tggtatgata cctggtatgg
     4981 gaaatgtaaa agaccaactt ggagatgtag atttgaatgg caaagaaatg aagagaacaa
     5041 aagccataat tcagtcaatg actacatatg agagaagaaa cccaggagta ttaaatgctt
     5101 caagaaagaa aagaatagct aaaggcagtg gaactagtgt acaagatgta aatagactta
     5161 taaaacaact taatgaaatg aaaaaaatga tgaagatgtt tgctggttca caaaagagta
     5221 tgaagaaaag gggcggctta ggcggtttac ctttttttaa ataaaatata aaaaagtaaa
     5281 aattattcgg aggtgactgt aatggcagtt aaaataagat taaaaagaat gggagctaac
     5341 aaaaaacctt tctacagaat agtagtagct gattcaagag ctccaagaga tggaaaattt
     5401 atagaagaaa taggatacta taatccaatt tctgaaccta aacaagtaag aataaatgat
     5461 gaaaaagcta taaaatggtt agctactggt gctcagccaa cagaagtagt taaaaaatta
     5521 cttgtaaaaa acggagtaat agaaaaattc gaagcttcta aacaagcaaa ataatttttt
     5581 cttggcaatt agtttaagta agggctcatt gctaaggtta ctttaggaga tgaaatatat
     5641 gaaagagcta gttgtagata tagctaaggc tctagttgat aatcctgact cagttgttgt
     5701 tgaggaattt gaagataatg atggtatcgt cttaaagctt acagttgctc aagaggatat
     5761 gggtaaggtt attgggaagc agggaagaat agctaaagct ataagaactg tcgtaagatc
     5821 tgttgcaaat agagaaaata taaaagtttc tcttgagata gtataaaata ggaggattag
     5881 gtataatgcc tagtcctttt tatataaatg cgtagtttaa gattttatga tgatatgacc
     5941 acataagatt aaatttttta agaaggtgat aacatgaaag aaaaattaac tcattttaag
     6001 gtaggtcaaa tagtaaatac acaaggttta aaaggtgaag taagagttta tccacttact
     6061 gatgatatag agaggtttga tgaattaaaa gatttctatc tgggaaaaga tttaaataat
     6121 aaattggctg ttgaaaaagt aagatacaaa ggttctatgg ttataatgaa gataaaaggc
     6181 atagacagca tagagaaagc agaaaaacta aaaaataaat ttatgtatgt atctagagaa
     6241 gatagcagag agcttgaaga aggcgaattt tttatagcag atatgattgg aatggaagtt
     6301 ttaactgtag atggaaaata tgtaggaata ctagaagatg ttcttcaata ctcagcaaat
     6361 gatgtctatg tagtaaaagg tgaggaagat aaagaattta tgataccagc tataaaaaaa
     6421 tttgtaccta caatagatat tgatgaaaga aaaatgatta tagaccctat taagggaatg
     6481 atagattagg tggtaatatg agatttcata taatgacact ttttccagaa atatttaatt
     6541 cgtacatgga tgaaagtata atgaagagag cagttgaaaa aggtataatt gaggttcata
     6601 tatataatat aagggatttt tctaataata aacataaaaa agtagatgat tatccatttg
     6661 gaggaggagc ggggatggta atgactcctc aacctatata tgacacatat aagcatataa
     6721 taacaactca taatataaat aacccaagtg taatttatct tactccaaaa ggtaaggtat
     6781 acaatcaatc tatggcaaag cagatgtctt taaaagaaga tataatacta ctatgtggtc
     6841 attatgaagg aatagatgag agaatcattg atttaatagt tactgatgaa atttcaattg
     6901 gagattatgt attaactggg ggagagctcc cagcacttat aatgatagat tctatatcga
     6961 gattaatacc aggtgtatta aaccaagaag aatcatttga agaagaatca tttaaagata
     7021 acttattgga atatcctcat tacactagac ctagagattt tgaaggatta aaagtaccag
     7081 aagtgctttt atcagggaat cataagaaga tagatgagtg gagaagagaa gaatctataa
     7141 ggataaccaa agaaagacga tttgatttat ataaaaagtc taatgaaaaa taaaaatata
     7201 atgaaaaatt aatatatatg tatacaataa agtacctata aatggaatta aaaacataaa
     7261 agtgcttgta ataactattg aagtatagta taatataaaa tgttgaaaat acgggcgttc
     7321 ctctgtttac actggtcggg tagttaagaa cgtctatgat atctaggagg aagaaagatg
     7381 aatgaaatgc taagagcaat tgagcaagaa caattaaaaa atgaagttcc taattttgga
     7441 cctggggaca cagttaaagt acacgttaga attatagaag gaaaaagaga aagaatacaa
     7501 gtattcgaag gtgtagtatt aaagagacaa ggtggaggag caagagaaac tttcacagtt
     7561 agaaaaatgt ctttcaatgt tggtgtagaa agaactttcc cagttcactc tccaaaaata
     7621 gaaaaaatag aagttactag aaaaggtaaa gtaagaagag ctaaactaaa ctacttaaga
     7681 ggtagagtag gtaaagctgc taagataaaa gaagctagaa ataaataatt aaaaacttta
     7741 cttataaatg gggggctgat ttatcagtcc tctttctatt ttaatgaaaa ttacagcaaa
     7801 taattatact agaaataaat agaaatattt gttgaaaata tacattattt atctatgaat
     7861 ttatagcttt taaaatgaaa aataaaattg gtaaatattg cattatattt tgtaacaata
     7921 ttaaaaataa gagaacagta ttatgtaaag tatgacgtta aaaagatgac aatattatag
     7981 tttgttaaga tagatttttt tagtggaatt aggatataat aatataatat taataaggag
     8041 tgatacatgt gtcaaatgaa tacgaagagt atcttatgaa tgacaatcta catataaact
     8101 ggtatccagg tcatatgaaa aaaactaagg agttagtaaa aaataactta aaattgattg
     8161 atgtagtaat tgaacttttg gatgctagaa tcccgtttag tagtaaaaat ccagacatag
     8221 acaggcttgt aggagataag ccaagagttg tcgtacttaa taagagtgat atggcagata
     8281 gggataaatt gaatcaatgg attgaatact ataagaaaat taatatcaaa gctataccag
     8341 tagatacaat aaaaggcgtt ggtataaata agataatcga agaatgtaaa aatgtaacta
     8401 gagaaaaaat gagttcttta aaagacaaag ggagaaaaga aagagctata cgaataatga
     8461 tagttggagt tccaaacgtt ggtaaatcgt ctcttataaa caaattaaca ggtagaaaaa
     8521 gcactcaaac tggagataag ccaggagtaa cgaaagggaa acaatgggtt agattaaagg
     8581 gaaatcttga acttttagat acacctggaa tactttggcc taagtttgaa gaccaagaag
     8641 ttgcattaaa tttagcattt agtagagcta taaaggatga gattttagat gctgaaactt
     8701 tggctttaag acttatagaa aaacttatga aaatagaacc tgagaagttg aaagccagat
     8761 ataagttaga ttgtctagga gaaactccaa ttgaaactat ggatatgatt ggccataaaa
     8821 gagggtttat aacaggtaaa aaagaactag attatacacg catagccaca actgtattaa
     8881 atgaatttag agatggaaaa ataggaaata taacattgga agttccagaa aacgttaaaa
     8941 gatagatttt aaaacaaaat caaaaattta atgttaaaaa tttaaaaaag ccttttagca
     9001 aaggcttttt tatttttttg aaatatagac aaatatctcc atataaaagt ataaattagg
     9061 ttagaaaacc tttaaaaata ttaatttaag acaaatatac ctaatatttt gaaaattaaa
     9121 atgagtgtga ttatttaaaa ttttagcata ttgacttgct tgtttcgtat aatatataat
     9181 aagcacaaat aaatacaata tattaaaaat ttaatttatt taattttatt aatttaataa
     9241 taagttctta ttgtttaatt caacttactt atgcaagcgt aaacaatcga gacattttat
     9301 taatttaata aggttaaaaa gggggatttt ataatgggta agaccaagga tgttatagta
     9361 tttggatttg cgttatttgc aatgttcttt ggagcaggta acttaatatt tccaccttac
     9421 ttgggaataa ttacaggtcc agaatggtta attgccttct taggatttac gtttgctgat
     9481 gctggattgg cacttctagc agtaatggct acagctaaat tcgatggaaa cgtagttgaa
     9541 atgtttaaaa gatgtggtat gaagttaggg attcttatag gttgtgcaga tattttatgt
     9601 ataggaccat tcttagctat accaagaaca ggtgcaacaa cttatgaaat gggtataatg
     9661 cctttatttg gaacttcaat tccagtgtta ttattctgta tacttttttt tgcgatttca
     9721 tatgtactaa caataagacc ttctaaggtc gtagatatag ttggacagtt tttaactcca
     9781 gctcttctaa tagctttagc atttataatt ataaaaggta ttatatcacc tcttggagat
     9841 atagtagata aacctatgat acctaatgta tttgcagaag gtataggtca aggatatcag
     9901 acaatggatg cttttgcagc aatagcactt gcatcagtgc taatagtatc tttaaatgat
     9961 aaaggatatt ctactattag tgacaagttg aaaatgattg gtaaggcggg tgttcttgct
    10021 tgtggaggat tagctctagt ttatggagga ttatgcttcc ttggggcaac tgtatcaaca
    10081 atgtatggaa cagatgcagt acaatcacaa gtaatagtaa atataactga aggattgctt
    10141 ggaaatgttg ggaaagcgat tttagcagta gtagtatcat tagcatgttt aactacatct
    10201 ataggtctta catctgccac aggtcaatat ttctctagac ttacaaaagg taaattgagt
    10261 tatgaaaaaa tagtattagc tgtatctgta ttcagtgcag tagtagcatc ttttggtgtt
    10321 ggaactataa taaagatagc atctcctata ctaagtatag tttatccacc atcaattgtt
    10381 ttaataattt tagctttctt caatgaaaaa attaagaatg ataatgtata caaaggagca
    10441 gtatatatgt cattgcttgt aagtatacta acagttatat ctagttatgg agtagctgtt
    10501 ccagtagtaa attctttacc tcttaattca ttaggattta actgggtggt gccagttata
    10561 atagctggaa taattggtaa ttttatacca tcaaagagtc aatctaatac acttggaaca
    10621 aactaagttt tgtttaatta aaatataatc aattgtaaat aaatgagtta actaaaagta
    10681 aaaggagtta tttgtacaaa taactccttt tatttttaga ttctatattt aattatttgt
    10741 atttaatatg gaaaaagtat aagaaaatat caaaaaaata tatagtttat tttttaacaa
    10801 taatagagat taaaaaagaa ttttagagta attttataaa tttaagtata catattaaat
    10861 gatttttaca aaaatataat ataaa
//
LOCUS       DAEMGG010000130        10471 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEMGG010000130 DAEMGG010000000
VERSION     DAEMGG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.131"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..808)
                     /locus_tag="KPK24_003153"
     CDS             complement(<1..808)
                     /locus_tag="KPK24_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2300802.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRR"
     gene            1225..2628
                     /locus_tag="KPK24_003154"
     CDS             1225..2628
                     /locus_tag="KPK24_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300803.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            2655..4199
                     /locus_tag="KPK24_003155"
     CDS             2655..4199
                     /locus_tag="KPK24_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG2300804.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKSGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            4230..5621
                     /locus_tag="KPK24_003156"
     CDS             4230..5621
                     /locus_tag="KPK24_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG2300805.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            5814..7310
                     /gene="abfD"
                     /locus_tag="KPK24_003157"
     CDS             5814..7310
                     /gene="abfD"
                     /locus_tag="KPK24_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG2300806.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            7445..7720
                     /locus_tag="KPK24_003158"
     CDS             7445..7720
                     /locus_tag="KPK24_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300807.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            7744..9051
                     /locus_tag="KPK24_003159"
     CDS             7744..9051
                     /locus_tag="KPK24_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG2300808.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            9071..10189
                     /locus_tag="KPK24_003160"
     CDS             9071..10189
                     /locus_tag="KPK24_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG2300809.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
BASE COUNT         3975 a         1185 c         2051 g         3260 t
ORIGIN      
        1 ttcttctcca aagaacccca atatttgcag taattgcacg ttctaatggt attgaacaga
       61 gattttctgt attttgaaat atttctttat acaatattgt tgaagccaag tcattttcta
      121 gtaaataacg tatggtagaa agttgatttg agtgtaaaat tatatttggc ttagcttttt
      181 caagtctttt tgtaataatc ttatagtgat aagaaccttc agataagatt acaaaaggta
      241 cattttttaa catataattc gatataatag gatgttttgc caattcattt ttgggatttg
      301 taactaagta taattctgtt ttatagattg tctttgaatc tatatcatca taaatatcac
      361 tgtcaattat accaatacaa aaatcaagtg tagattcttc aatcatttta gctccaacaa
      421 atgttggaac ttcaaaaatt ttaagctcta tatctggata aattacatta aacctaggaa
      481 gtatttttga aagcaaaaac gtacctaaaa ctgtaggaat acctattttg agagaagctt
      541 ttctcttttt tcctaaatca agtgcgtcac attgaaaatc ctcaaattgt tttataaaat
      601 gttttgactt gtctaaaaaa atttttcctt caggcgttaa ctctatttta ttacctattc
      661 tgtaaaaaag ttcaaagcca agttcttttt ctaattcttt aattgctaat gttatggatg
      721 gttgagaaat atgaatactt tccgcagcct tagtcatgtt tttatattta cagacagttt
      781 gaaaatagta taaatagttt atattcattt ggtattagtt cctttcattt acatattagt
      841 gacaagtata taattaacaa tagcataagt tttgtatatt gttgtaaaaa aatagaatag
      901 ttttaaacta tcatagcata agtttttgat attttcaatc tattaagaat agtgctatta
      961 ttaaattaac aaaagagaat agctggtatg gtttagagta aatcaatcta tattacagtt
     1021 atatcaacga atatcaaaaa attatctatt aaaaaataaa aattattttt acaattaaat
     1081 tgttaattaa atcataaaga aaaaagtgat attaacaaga taaaattttt ttaatcagaa
     1141 atgttaattt aataacaaaa tagagtattg gggagaaagg agaaatatat tttttaataa
     1201 tatttgttaa tttaataacg aaaaatgaaa gggagtgatt tagtgaagtt tgaattgatg
     1261 gattttttaa tgaatccttt tgtccttatg tttgctgcag taattacagg aattttattt
     1321 ggaaaaatta aatttgggaa gtttaatttt ggggtatcag gagcattatt tacaggatta
     1381 tttattggat ggcttgcata tagcttgggg aatctaataa tagcaaaagg ggaaacagca
     1441 gcaggatata aggcagcaac tgtaatgatg gggaatggaa ttatttcttc tgacttcttt
     1501 gatttctttt taataatatt tgttgcagca gttggtctat tggcagcaaa agatatgaaa
     1561 gctgtattaa agaagtatgg agcaagattt gtaattttag gagttcttat aacttttata
     1621 ggtggctcta tgacttatgc aatgacttta ctatcaagtg ataaaggaag tagtgcttat
     1681 gaagtttctg gcgtttacac aggagcatta acaagttcgc ctggattagc tgctgcttta
     1741 gaaacagctg gtaaacatgc agaagatgta tcaaaagaat ttgaaaaagc ttcaataaaa
     1801 gataaaaaag aaattttaaa agtagtcgac ccagaaggca aattggatgt gaatacaact
     1861 acatcactga ctcaagagca aatagataag tatattgcat atgctgaagc tggtgttggt
     1921 ataggtcatg cagtcgcata tccttttgga gttttaatag taatcttagg tgtaaatttc
     1981 cttccaaaat tatttaggat ggacctaaaa gaagaaagaa gaaaatatga gaaggaaatg
     2041 aaggaagcaa gagatagtgt ttctggtaaa acgataccag aagttccatt taatataata
     2101 acatttttct tgacatgttt agctggatac ttagttggag gaatacatgt atttatggga
     2161 ccactagggt acttcacact aggtgcaaca ggaggttcac taattgtatc tctagttctt
     2221 ggatatatag gtaagatagg ggtagtaaat ttcagaatgg aagaaaaggt tttaaatatt
     2281 ttaaaacaaa tagggctagt gtttttctta gcaatagttg gattaagata tggtggaaaa
     2341 gtagttgatt caattatgac atctggtatg cacctagcct tagtagcaat agcagtaggt
     2401 gttacagcta tgatgatagg attcttagtt ggcaaatacg tatttaaatt aaattggatt
     2461 ttattatctg gagctgtctg tggtggtatg acatcaacac caggtcttgg agctgctgtt
     2521 gatgctcttg atagtgatga cccagcagca ggttatggtg ctacgtatcc atttgcatta
     2581 ttaactaaag ttatattggt tatagtttta cacaaacttc caatgtaaat aagattttat
     2641 atggggggat atttatgaat aatgaaggag catttgaaga attaagtgga agattaagaa
     2701 ataaagaatt gtgtgattta ataatgaatg cagatgaagc tgctaaaatt atagaggatg
     2761 gcatggttat tggagttagt ggatttacac cttcaggcta tccaaaagca gtaccattag
     2821 cagtttcaga aagagctaaa agtggagaaa agataaaatt aaccgtatat tcaggagcat
     2881 ctttaggtcc agaagttgat ggagcatggt cagaagcagg tataattgaa agaagacttc
     2941 cttatcaaac taattcaata cttagaaata atattaataa aggtgttgtt gattatatag
     3001 atatgcacct aagtcattct actcaatttt taaattatgg aactatacct aaagtagatg
     3061 tagctatagt agaagcactt gcaataactg aagaagggaa tattatacca actagtggga
     3121 taggtaattc tccatctttt ataaaaagtg cagataaagt aatagtagag ataaatcttg
     3181 caaaaccaat ggaaatggaa ggtatggcag atatatatat tacggaaaat ccaccaaata
     3241 gaaaaccaat agaaataaat catcctaagg atagaattgg aacaacatat ataccttgtg
     3301 gtttggataa aattgcagga gtagtaatta ccaatatgca agataagacc cgtccattgg
     3361 gggttgtaga tgaagcttcg aaaaaaatat caaataatat tattgctttt ttaagagaag
     3421 aagtaaagtc aggcagatta tcaaagaatt tacttcctct tcaatctggt gtaggaagtg
     3481 tagcaaatgc agtattatat ggattatgtg aatctgaatt tgaaaattta acttgttata
     3541 cagaggttgt acaggactct atgttggatt taataagaat gggtaaagtt acaatggcat
     3601 ctactacatc tgtaagtcca tctccagaag gattaataaa atttgaaaaa gatattgatt
     3661 tttttaagga taagattata ttaagacctc aagaaattag taacaatcca gaaatagcca
     3721 gaagaattgg tgtaattgca atgaacactg caattgaagt tgatatttat ggaaatgtaa
     3781 attctactca cataatgggg tcaaagatga tgaatggaat tggtggttct ggtgattttg
     3841 ctagaaatgg agcaataact atatttagta ctgagtcaat tgcaaaaaat ggtgatatat
     3901 catcaatagt acctatggta tctcacgtag accacactga acatgatgta atggtaattg
     3961 taacagaaca aggatatgct gatttaagag gtcttgcacc tagagaaaga gctataaaaa
     4021 taatagaaaa ttgtgcccat ccagattata aagaacaact tagggattat ttaaatagag
     4081 cttgtaaaag tggagcaaaa caaacacctc acatattgga cgaagcttta tcatggcata
     4141 gtaaatttat gagtacaggt acaatgaaaa aggctgaaac ttttaaaagt gcattataaa
     4201 atatttaata aattggaggt agattttaaa tggaaaaagc agtggaaaac tttgaagatt
     4261 taagtaagga atacattaat ggttatattg agagagcaag aaaagctcaa agagagtttg
     4321 aatgttatac tcaagaacaa gtagataaaa tagtaaaaat agttggaaaa gtagtgtatt
     4381 ataatgctga atatttagct aaacttgctg tagaagagac tggaatggga gtatatgaag
     4441 ataaggtagc taaaaataaa agcaaagcaa aagttatata taacaattta aaagataaaa
     4501 aatcagttgg tataattgat atagatagag aaactggaat aacaaaggta gctaaaccag
     4561 ttggagtagt agcggcaata actccttgta caaatccaat cgttacacct atgagcaatg
     4621 caatgtttgc acttaaggga agaaatgcaa ttattatcac acctcatcat aaagcaattg
     4681 gatgtagtac aaaaactgta gagatgatta atgaagaatt agaaaaaata ggagctccag
     4741 aaaatttaat acaaatactt gaccaacaat caagagaaaa tacaagaaat ttaatttcat
     4801 cagcagatgt agtaattgcc actggaggaa tgggaatggt aaaagctgcg tactcaagtg
     4861 gaaaaccagc acttggtgta ggggcaggaa atgttcaatg tatcatagat agagatgtag
     4921 acattaaaga agcagtacct aaaatcatag caggaagaat ttttgataat ggtatcattt
     4981 gttcaggtga acagtcagta atagtagcag aagaaatgtt tgataaaata atggatgaat
     5041 ttaaaaataa taaaggtttt atagttagag ataaagtaca aaaagaagct ttcagaaatg
     5101 ctatgtttgt aaataaatct atgaataaag atgcagtagg tcaatcagtt catacaattg
     5161 caaaaattgc aggtgttgag ataccagaag atacaaaaat aatagtaata gaagctgatg
     5221 gaccaggaga agaggatatt attgcaaaag aaaaaatgtg tccagttata tctgcataca
     5281 agtataaaag ttttgaagaa ggtgtagcta ttgcaaaagc aaatttaaat gtagaaggaa
     5341 aaggacatag tgtatctata cattcaaata cagttaaaaa tatagaatat gcaggagaaa
     5401 atatagaagt atcaagattt gttattaatc aatgttgtgc tactagtgct ggaggaagtt
     5461 tctttaatgg tcttgcacct actaatactc taggttgtgg ttcttgggga aataacagca
     5521 tctctgaaaa tcttgattat aagcatctaa ttaatatttc tagaattgct tattatatgc
     5581 cagaaaatga agtccctaca gatgaagaac tttggggata aaatttagac aaaaaatatt
     5641 cattctaaat taaagtagga atatatttta atttagaatg aatttaagac ataaattaaa
     5701 tttaaattag tttattggac taaatttaaa atattaagag acatatagaa gaaggaagta
     5761 ttataattta aaataaataa aaaaatctat aaaagagaag gagtggtttt attatggcat
     5821 taatgacagg agctcaatat attgaaagtt tgagaaaatt aaatactaag gtttatatgt
     5881 ttggtgaaga ggttaagaat tgggttgacc atcctatgat tagaccatct ataaattgtg
     5941 tagcagcaac ttatgactta gctcatgacc cagaatatgc agatttaatg acagttactt
     6001 caaatatcac aggtgaaaaa ataaatagat ttggtcatct tcatcaaagt gttgatgatt
     6061 tgataaaaaa agtaaaaatg caaagactat gtggtcaaaa aacagcttct tgtttccaaa
     6121 gatgtgttgg tatggatgcg tttaatgcag tgtattcaac aacatttgag tgtgataaag
     6181 cccatggaac taattatcat gataattttg taaaatactt aacttatata caagaaaacg
     6241 atttagtagt tgatggagct atgactgacc ctaagggaga tagaagtcta tcacctagtg
     6301 ctcaacctga cccagatatg ttccttcata tagtagaaag aagagaagat gggataattg
     6361 taagaggagc aaaggcacat caaactggtt caataaattc acatgagcac cttataatgc
     6421 ctactataag tatgactgaa gcagataaag actacgctgt atcatttgca gttccttcag
     6481 atgctgaagg agtgtttatg atttatggaa gacaatcttg tgacactaga aaattagaag
     6541 aaggtgcaga tgttgattta ggaaataagg agtttggagg tcaagaagct ttagtagtat
     6601 ttgataatgt atttgttcca aatgatagaa ttttcttatg tggagaatgg gatttctctg
     6661 gtatgttagt agagagattt gcaggatacc atagacaaag ttatggtgga tgtaaagtag
     6721 gagtaggtga tgtaataata ggagcagcag ctttagcagc tgattataat ggagcaaaca
     6781 aagcttcaca tataaaagat aaactaatag aaatgactca ccttaatgaa agcttatatt
     6841 gctgtggtat agcatgttca tcagaaggtc acaaaacaga agctggaaac tatcaaatag
     6901 atttacttct tgcaaatgtg tgtaaacaaa atgtaactag atttccttat gaaatagtaa
     6961 gacttgcaga agatatagct ggagggctta tggttactat gccttcagaa aaagacttta
     7021 aatcagacct taaagttgga acaagtggaa tgactattgg cgaagtatgt aacaagtatt
     7081 ttaaagcttc ttcagtggct tcaacagaag aaagaatgag aatattaaga ttcttagaaa
     7141 atatatgcct tggttcatct gctgttggtt atagaactga gtcaatgcat ggggcaggtt
     7201 caccacaagc tcaaagaata atgatttcaa gacaaggaaa cataaatcaa aagaaagaat
     7261 tagctaagaa aattgcggga ataaaaaaag aggaagcttt aaatttataa aaagttgata
     7321 tataaaggga aattatatat taatgtgtat taattaatga aaaaatatgt agtataagag
     7381 atagttataa aattatggtg gtttttagct aagtaaagaa atttttaata aggagaattt
     7441 acacatgacc tgtggagtta gattttgtgg gggctgtaac cctagatacg aaagagggaa
     7501 ggctcttaca ataatcaaag agcacttcaa tggtagggta gattttagta tagcacaaga
     7561 gaacgtcgag tatgatttgc tacttataat tggaggatgc gcaactctat gcgcatccta
     7621 tcaacaatat aaagtgaaaa atgaatgtgt taagatgtgg gattttagcc atatagaaag
     7681 agttataaat gtgattgaat cagcaatttg tgaaacttaa aaaagctaaa tggggggatt
     7741 tcattgagtt ggcaagaatt gtatcaaagt aaattatgta gtgctacaga agctgtaaaa
     7801 cagattaaaa acggtgatac tgtagtattt gcacattgtg taggtgaacc tcctgcacta
     7861 gtagaggcta tgattgaaaa tgctgaacaa tataaagatg ttgagattaa acatatggta
     7921 agcttgggta gtggtggata tactgctaag ggaatggaag ctcattttag agtaaatcca
     7981 atgtttgtaa gtggaaatgt aagaaaggca attgaaaatg gagatggtga ttttacacct
     8041 gcattcttcc atgaagtacc aaagttgctt agagaaaaaa gattaaaatg tgatgttgtt
     8101 ttagcacagg taactccacc agatgaacat gggtattgta gcttaggaac atcagttgat
     8161 tatacttatg aagccattaa aactgctaga acagtaattg ttcaagtgaa tgaccagttt
     8221 cctagaacct atggtgaggt agtccatgta tcagagtttg actatatagt ggaaaaatca
     8281 caacctttat ttgaactaca acctgcaaag attggagaag tagaagaagc aattggtaaa
     8341 aattgtgctt cattaattga agatggttct actttacagc tagggattgg tgggatacca
     8401 gatgcagtta tgttattttt aactgataaa aaggatttag ggattcattc agaaatgatt
     8461 tcagatggga ctttggccct ttatgaaaaa ggtgttataa atggtaaata taaaaatttt
     8521 gataaagaaa aaatgacagt taccttctta atgggtacta aaaaattata tgactttgca
     8581 aataataacc ctgcagtaga ggtaaaacca gtagactatg tgaatcatcc agcaattata
     8641 atgaaacaac ataagatggt ttctataaat tctgctatac aagttgattt aatgggacag
     8701 gtagttgcag aggctatggg acttagacaa tttagtggtg ttggaggtca agttgacttt
     8761 atacgtggag tgtctatggg tgaagatgga aaggcaatta tagctatgcc ttcaataact
     8821 acgaaaaaag atggtacagt aatttcaaaa atcgtgtcta ttgtggatga aggtgcacct
     8881 ataacgacat caagaaatga tgttgactat attgtaacag aatacggtat agcagaatta
     8941 aaaggaaaat ctcttagaga aagagcgaga aatttaataa atatagctca tccaagtgta
     9001 agagaaagtc tagcagtaga atttgaaaag agatttaagg agaaatatta ataatagagg
     9061 ggtgttaata atggaagcac ttagagtagt tccaaagata ttttactttg atacatttaa
     9121 agaatttaat gaagaattta aaataggaaa aaatgattta gtaattacta atgagtttat
     9181 atatgagcca tatatgaaac ctcttggaat agatacaaat ttaatttttc aagaaaagtt
     9241 tggaacaggt gaaccatctg atgaaatgat agactccatg acaaaggaaa tgaagaaata
     9301 caattttgat agaattattg catttggagg aggaacaata gttgatatat gtaagatatt
     9361 agcacttgat gttccagaaa agtctattga tttatttgaa ggagaagtat cacctaaaaa
     9421 agtaaaagag ttagtagtag ttcctacaac atgtggtaca ggtagtgaag taacaaatgt
     9481 agctattgca gagttaaaat ctaaacatac taaaaagggg cttgcagtag aagaaacata
     9541 tgcagactat gcagtgttaa ttccagagac agtaaaaggg ttaccttaca aattctttgt
     9601 tacaagttcg gtagatgctc ttatacatgc tattgagtct tatctttctc caaaagcaag
     9661 tccatttaca gagatgtatt ctctacaagc aataaaaatg attatggatg ggtataagaa
     9721 gatagttgat aaaggagaag aagagagatt taatcatctt agagactttg tacttgcatc
     9781 aaattatgca ggaattgctt ttggaaatgc tggttgtgca gcagttcatg cactatctta
     9841 ttcaataggt ggagctttcc atgttgcaca tggtgaagca aattatcagt tctttactga
     9901 agtatttaag atgtattcta gaaaaaaacc taatggaaaa attataaaat gtacaaaaat
     9961 tttagcagat gcccttgaat gtgaccctaa ctgtgatgtt tatggagaat tagaaaagtt
    10021 tttaaataaa ttaatagcta aaaaagcttt gagagaatat ggaatggtgg aaagtcagat
    10081 agatgaattt acagatagta caatagcaaa tcaacaacga ctacttgcta ataattatgt
    10141 agagcttagt agagaggaaa taagagagat atttgcaaat ctttattaat acaatttttt
    10201 atttatatgt atagtcaaca taaacaatgc taattatatt tatgtacaaa taaaaataag
    10261 ggaacttaaa ttttaattaa agttaagttc tcttattttt atatggagtt aaatataagt
    10321 atatttttaa tttaaaatat gcttatattt aagtctatta ctttatttga aacaatttgt
    10381 agaatttcaa attccttgac agtaataagt aagtaattgt ataatcaaac tgcaatatat
    10441 taataatatt agtgaaaatg aaaggattat a
//
LOCUS       DAEMGG010000131        10434 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEMGG010000131 DAEMGG010000000
VERSION     DAEMGG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.132"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            185..2041
                     /locus_tag="KPK24_003161"
     CDS             185..2041
                     /locus_tag="KPK24_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG2300810.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    2124..2364
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2430..2665
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2749..3966
                     /locus_tag="KPK24_003162"
     CDS             2749..3966
                     /locus_tag="KPK24_003162"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG2300811.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            4083..4577
                     /gene="cdeM"
                     /locus_tag="KPK24_003163"
     CDS             4083..4577
                     /gene="cdeM"
                     /locus_tag="KPK24_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG2300812.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGENRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            4842..6107
                     /gene="hisD"
                     /locus_tag="KPK24_003164"
     CDS             4842..6107
                     /gene="hisD"
                     /locus_tag="KPK24_003164"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG2300813.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6310..7062)
                     /locus_tag="KPK24_003165"
     CDS             complement(6310..7062)
                     /locus_tag="KPK24_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2300814.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7180..7449)
                     /locus_tag="KPK24_003166"
     CDS             complement(7180..7449)
                     /locus_tag="KPK24_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300815.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7924..9018
                     /locus_tag="KPK24_003167"
     CDS             7924..9018
                     /locus_tag="KPK24_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG2300816.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISNITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            9020..9970
                     /locus_tag="KPK24_003168"
     CDS             9020..9970
                     /locus_tag="KPK24_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG2300817.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKVDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9963..>10434
                     /locus_tag="KPK24_003169"
     CDS             9963..>10434
                     /locus_tag="KPK24_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300818.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCK"
BASE COUNT         4228 a         1021 c         1865 g         3320 t
ORIGIN      
        1 ataagctact gtaattagat ttagaaaaaa gcgacataaa taattataaa caaataataa
       61 attacataaa attaaattta ttcacaagta tatcttataa aatatgttat aattaataga
      121 aaattgtcat ataattaatt aagtagtatt attataaata acattgaaag gtagtggtta
      181 gaatatggat acccataata aatatgtaaa ttttataaaa aatatacctg tacctttttt
      241 atattgtaga attgtaaaga gacaagaaga tatagaatat cgagtagaat atataagtaa
      301 gggtatgggc aaagtattac aattagaaga aggtatttgt gataaaaata tactagatgt
      361 attgcctgta tttaagtcta aaaaatattt caaagagttg ttctcaaatg aagtggattg
      421 tataaaaagg tacattccga cacttaaaaa ctggataaat ataaaaaaac agataatagg
      481 ggattcatac ataattttgt attttggtaa aattgtattt gattatagac agataatcga
      541 ttcctttgat aaaaaggaaa aagtagctta tattaaagac gaagaaggca tatatataga
      601 ttgtagtgaa aatttaatac caatattaaa taacaacatt aaaacaacga aagatatctt
      661 tggtaaaaat gatatagaag tatggggaga gaatacagga aaattattta gagatgatta
      721 tagagaagga gtatctagta aaaaaagatt tttacaaaat ctttttgaat atgaagaaac
      781 attttttatg gttgaaaaat attttttgta tgatgaagac gaacttttag gtactatagg
      841 gatagtagac aatataatat atagtggtta cagtaataga aattataact caaaagattt
      901 aatgaagatg atagaacact caattccaga gaatatgttt tataaagatg tgtatggaaa
      961 ttatattgga tttaattcag gttttttgaa tttagcttgt atgaataaag aagaattatt
     1021 agggaagaat agttataaaa tatcagaaga agaagcttta atagataaga tatttgaaag
     1081 tgataagggt gtagttgaaa ataaaaaagt agttacattt gagttaaata taagtatgaa
     1141 tgatgaaaac aagtgtattg agattacaaa aaggccgttt tttgatagct atggaagtgt
     1201 tattggaata attggaacag caagggatat aagtagaaga aaaagattag aagaggaaat
     1261 ggataaaact aggatggaat tttttgcaaa tttatcccat gaacttagaa cgcctataaa
     1321 tttaatatca tcttcattac aagtgataga aaaaaaagaa gcagatttaa tagaatcaaa
     1381 tgatacttta aaaagaaatc taggtataat aaaacaaaat ggaaatagaa tattgaggtt
     1441 agtaaataat gttatagact ttacaaaaat gcaatcagga tatttagatt ttaaaccaga
     1501 agaatctgat ataattgcat ttatagagga gatatgtatg tctgtagctg attttgcaag
     1561 ccaaaataat atacaactta catttgatac agaaatagaa gaattttcaa tgttatttga
     1621 ttcagaaaaa ttggaaagaa taatattaaa tttactttct aatggaatta agtacaataa
     1681 gaaagatgga aaaataaata tatttttata tgtgaaagat aatgtattta atatgaaaat
     1741 atctgatagt ggtataggta taccaaaaga aaaaatagat aaaatattta ataggtttga
     1801 acagattgac aatgaacttt cttacagagt taaaggaagt ggtattggac tttcattagt
     1861 gaaatctttg gttgagttgc atgagggaag tatatcttta aaaagtcaac ttggtattgg
     1921 aagtgaattt atagtttcat tgcctgttag aagtaaaaat aatattgaaa aatataatca
     1981 taaaagagaa atcagcaatg aactaagtaa aaaattagaa atagagttct cggatttata
     2041 agtttaaata taaaaaataa aattttaaaa aagtattgac aattgattta gaagggttta
     2101 aaataaatta caacataata aataaaaagg cgatgaatag gaagagtaaa tctaattcga
     2161 ttaccagaga gaagagtccg ttggctggaa gactttttaa attagaggga ttgaaaacta
     2221 cctaggagct actttctgaa gtcatctgca ttagtagatt ttagtaagaa tagtcgttag
     2281 ctgcgttaaa gtgtaaagtg agttttcatg acatatgaaa actaaattag gtggaaccgc
     2341 gggaataata ctctcgtcct tatgttataa ggatgagagt tttttgttat ttaaaaatat
     2401 aaacagtaat cataataatt atagaaaata taaaagcaat gaataggaag agtaaattta
     2461 attcgactat cagagaggaa agttcacagg ctgaaagact ttctaagttt gagaaattga
     2521 aaactaccta ggagctgtgg tctgaagtta ctttaaaaag ttttagtaag actaaccgtt
     2581 aactgcgtta aagtgtaaag tggattttat caataaatga taaaatcaaa ttgggtggaa
     2641 ccgcgagtat agactcgtcc ctttatatag ggatgagtct atttttattg caataaaaga
     2701 ttgaaaataa aaaaaatcgg aaaattataa atttaggagg aaaataaaat ggagaataaa
     2761 gtgaaaattg gagtactagg atatggagtt gtaggaagtg gacttataga tataatagat
     2821 aataataaag aaaaaagaaa tatagaaata gttggaattt tagttaataa tttggaaaaa
     2881 cataaggata aaaaatattc taatataata actaataata tagatgatat atttaataaa
     2941 gatatagata tacttgttga agtcatgggt ggtttagaac catcattatc ttatataaaa
     3001 aaagcattaa acaataaaat tcatgttgtt acagcaaata aagatttgtt agctgaatgt
     3061 ggagatgagc ttgcaaaatt agcaagtgaa aataaagtaa gtattaaatt tgaagcttca
     3121 gttgcaggag gtataccagt attaaaacca ataatagaat cactagaagg aaataatata
     3181 gatagtataa atgcaatttt aaatggaact actaatttta tattatctaa aatgtatgat
     3241 gagaatctat cttatgatat ggcattaaga caagctcaag aattaggttt tgctgaagca
     3301 aatccagagt cagatgtatt agggtatgat gctgctagaa aactatcaat attatcaact
     3361 ctagcttatg acaatagagt atattggaag gatgtgtatt tagaaggaat tacagatata
     3421 gatgaaaaag atattgaata tgcaaaaaaa ttaaattgta aaataaaatt gattggtcaa
     3481 agtaagtatg aaaatgataa agtaagtgct tttgttagac ctgttttagt agaaaaagat
     3541 aatatccttg ctagaataga caatgagttc aatgctgtca tagtgaatgg agattcagta
     3601 ggagaagttt cttttgtagg taaaggcgct ggaagtttag caacaggaag tgcggtttat
     3661 tcagatgtta tagatataat agataataga gtttcaagta tagattcttt tactaaagat
     3721 aaaatacaag ttaataaaat agttagagaa aagtgtggag cacttcttag atttaaaaaa
     3781 tgtaataaag atgaaattct aaatatagtt gagaattgtt tagtaaaatt cgatatttta
     3841 aatgatgatg atgaacttgc tattatggtt tatgctgatt ctgaatatga aataaacaac
     3901 agtttatgtc taataaaaga taaaggatac tgcgaaaaaa tgaataaaat gttgaaaata
     3961 agttaaaaaa gaaaaaatat aagtttatat gtaacatatt ttgacgattt taatactata
     4021 tagtatgaga tgaaacaaaa catctcataa aaaaattaaa attcaaggga gagatatgtt
     4081 atatggaaaa taaaaaatat gcaaatggtg gttattcaga agactggtat gaaagaggag
     4141 aatctacagc taaatggttc cagaatgata gagaagaata tgaaagagca gcatacgatg
     4201 aagatagaga aagaagaggc tcaaactgtg gatgttcaga ttcaggagaa aatagaccta
     4261 gaaactgtga aagatttaga agagaagcag agataagaga aagagaagca agagaagcat
     4321 tctgtgaatc ttcagagaga aagaaagaag cactagcata tgaatgtgaa gctagaaaat
     4381 tatgggaaga agcagaaaaa tactgggatg aatattcaaa atacaactat aaaggaatcg
     4441 aatatttagc agaagctgct agattatttg atgaaggtat ggaatgtgaa gctagaagaa
     4501 atggaaataa tggaggaaac aataataatt gttgccataa atgccataaa tgtaattgta
     4561 actgctgtag aaaataaaaa aattaattga caaatcaata gttaaaatat atcatattat
     4621 gtataataaa gagaaaattc agaaaaaaat aatgattaga agaaatataa gtatatttat
     4681 tagacaaata gtgctcagtt agtgaggtga gtacaatatg attaaactcc catcctctag
     4741 cttttgatag agggtgggag tttttattta tttatatatc ttaaatgagg ataatattgt
     4801 aaaataaatg agtatgagat tgtaaaatta ggaggtaaaa aatgaagagt attaaaacat
     4861 caagtatgaa agaaaaggta gatgaaaaga tattaagaga gcaagtaagt tctataatag
     4921 aagatataag aaataataag gatgttgcac ttaaaaaata taatgagaag tttgatagaa
     4981 atacaagaga tgaatttaga attactaagg aagaaataaa ggaagcttat aagcatgtag
     5041 atgacgaatt tataaataac ttaaagattg ctgccaaaaa tataagagaa tttgctaaag
     5101 ctcaaaaact aagttttgaa aacccttttg aaaaagaaat ttatccaggt gtaatcttag
     5161 gtcaaactaa tattccaata gagtcatgtc ttgcatatgt tccaggaggt cagtatccat
     5221 tgttttcaac agctcttatg cttataatac ctgcaaaagt agcaggagta aaaagaatag
     5281 tggcatgttc accaactatg aaaaatactg aaaagataaa tcctaagaca ttggtagcta
     5341 tggatatagc aggtgctgat gaaatatatg caactggagg agttcaagct atagcagcat
     5401 ttacatatgg aacagaaaag attaatccag ttgacataat tgtaggacca ggaaataagt
     5461 ttgtaactga agctaaaaga caatgttatg gacaagtagg gattgatttt gtcgctgggc
     5521 caagtgaagt tcttataata gcggatgaaa cttcaaatcc agtttatatt gcagctgatt
     5581 tattagcaca atgtgagcat gatttaaatg caagaggaat attattgaca aatagcttgg
     5641 aagtagcaca aaaagttgaa aaaaatatag aaagtatgct aaaagatttg ccaacaaagg
     5701 acatagcata tagttcatgg gaaaataatg gagagataat attggttgat gatatggaag
     5761 aagccattaa aataagtaat ttttatgcac cagaacattt agaagttgca gtaaatgagt
     5821 ataatgatat ttgtgataga ttaactaact atggttcttt atttattgga aatctttctg
     5881 ctgaggtgtt tggtgactat gtttcaggta ccaatcatac attaccaaca cttaaagcat
     5941 caagatatac tggtggtgtt tgggttggaa cttttatcaa aacttgtaca aagcagatat
     6001 ttaatgaaaa agctatacaa tcattagcac ctgtagctga aaagctggca aaagaagagg
     6061 gattatatgc acatgcaaag gctgctgagg ttaggtttaa aaagtagtaa aaaattttat
     6121 tttggcagtc gagatttagt gttagactga gataaggaaa acgctgagaa cccgaataaa
     6181 tactatattt tgtgggcctc ggcgtttctt ttatatctgt atataatagg ataatactac
     6241 ctgtgtgttt aaatgctagc ttaatatttc ttttctaaat aatgtttatg aaatagttaa
     6301 tatttttagt taatacttga aaaaaacttt tgctaactgt gcaaattcat ctgcattcgt
     6361 caatatagca gggtgtcctc cctgactaaa aatgtgaata cttgcttttg aaataaaagt
     6421 tgccatttgt ttgtactctt cttcaagatt atgacgacac atttcgtctt ccttacttcc
     6481 tgtaaataaa attggcattt ctaatttaca caattcttta tgaaacaaag ggcgtttctc
     6541 gtttgcacat tgcaatagag cttttgtatc aagattaaca acattctccc aatcttttcc
     6601 atgacaccat tcatagaatt ttcgtgcttg aatatcttgc tttgattttt cccttccagt
     6661 gattagatta tctatgaagt tatcattaag agtacgtcca tcaaagctat ctgctataat
     6721 tgcttcaact aaatcgggac gttctaatcc agtattaaca gctgcccatg ctcctccact
     6781 tgttccaatt aagccaactt taccacaatt caaatgttca atcaatgcta ttgtctgtaa
     6841 ggcttcatca tgccatatgt caggagaaaa ttgagaaact ctatccgact gtccattacc
     6901 taaaaaatca attaagatac atctaaaatt ttctacatat aatggcacaa gtgctttaaa
     6961 catattagaa gaaccagtat taccatgcag gaaaattatc ggtttacctt gtccatattc
     7021 tttataaaaa caagaattac cttgatactg aaaatatgac atatattttc ctccttttta
     7081 tttgttaagt taattataac aatataggta tgaatgaaaa acctcatgtt ctttcttaga
     7141 ataaaaatgt gccacgcata aaacgtgaca catttctaat tataaatttt gctgtttaag
     7201 tattctttgt atgcttttct ctgtcaaaaa gtatttctct gataactgct tgatactcat
     7261 tccattttgg aaatttatac agatttgaga attacgagaa gctaagactt gtttggtgtt
     7321 tgtgttttca ccccaatgct ttttgtgctc ttgctttttg ggaatataaa tcactttacc
     7381 atctatatat tcttgcactt gctcaatcag ttctttaggc agtaagtcaa tcgaattttg
     7441 atagctcatt ttgcacctcc taaataatta ttctttagga ttagcaaagg ctactgccac
     7501 tcacaattaa aaataaaatt ttttattatg tacaatagcc tttgctatgc acaagttcca
     7561 attcccataa actaaatact cctttctggt ttgaaaaatt catctgaaca ttgatttcgc
     7621 ttttaagata gagttcaatg acaaatttac atttttatta tatatattca gatagataat
     7681 tgcaactgtc tttgtatgag gatattttat tatagtcaat actataatat gtatttatat
     7741 catcaaacta atatgaacag ttgaaaggaa tatgaacaat aaaacttagt caggaagaat
     7801 aattatgaag cagacaatag agaaatttga ttttaaagtt ttattgaaaa tttattgaca
     7861 aatttaactt gtaaatatta tactaaactt aataaataaa gtttataaag taaggtgttg
     7921 tctatgaaaa atatagttgg aaaaccgcaa acgataaaaa aagtaaatga agatttaata
     7981 aaaaatatta taaaaaatga aggtcctata acaaaaccag aaatttcaaa cattactgaa
     8041 ttgagtttag ctacagttaa taagattgta gagcaattag ctttgaaaaa tgaagttaaa
     8101 gtaagtggac taagtgaatc tactggagga agaagagctc aattatttga aataaatgca
     8161 aacttagaac acataatagc tttatattat tatagaaatt gttgtatagg ggtagttgcc
     8221 aatttattag gtgaaataat ttatcaagaa gaatttaata taagaatgga tacatatgaa
     8281 aacgttaacc aagatacttt ttttgttata gatactctaa taaaaaagga aaaaggaaac
     8341 aatataaggg ctattggaat tggtgtccca ggagttgtaa aaaaaggtgt agttaaaaat
     8401 ataccaacta taaaaagttg ggaaggtata aatttagaaa atttaataaa gagtaaatac
     8461 aatactaaag tgtttataga aaatgataca aatattacta ctattgggat ttatcaaaaa
     8521 cagtataaag acaaatataa aaatatggct cttatgtatt tagaagatgg tattggttca
     8581 ggtatagtta taaatggtga attgtattta ggttctacaa attttgctgg agagttaagt
     8641 tatctaaata tagaatttgg taagtataac tctaacaact taaatcttga agattatatt
     8701 atgagtttaa gaaaaagata tttagaaagt aaagataata aatttaagaa agaaatttta
     8761 tctataatat caaactttat aaggactctt gtatgtgtcc taaatccaca agctatgatt
     8821 atacagtgta gtatattaac caagagtgac atggagttta tagaaaaaga tgtaagcaat
     8881 aatgttggag aagaaaattt tcctaaatta ataaaagtag agtcattgag agaaggaagt
     8941 ataaatggag taattagtat gtgtttaaaa gaaactgact atcaatattc cttatctaat
     9001 aaaaaaggag gatattaatg tgaaatattt ggtttcaata gatggagggg gaacaaaaac
     9061 taaattttgt gtaagtgact tagatggaaa catacttaaa gaacatacca ctggttcaac
     9121 taattataag tcagttggaa ttaaaaaaac atatgaaaat attaataatg gatttaaaaa
     9181 aatattaaaa gatttatata tcgattatga cgatattgag tacactgtat ttggtatatc
     9241 tggttgtgat tctcctaatg attataagat tataatggat gaaatattaa aaattgggat
     9301 taacaaagaa aaaatatatc tggcaaatga tgcagtttta gctttttatg cacaagcgga
     9361 ttcccctggt ttagtaattg tggctgggac aggttcaatt atactgggaa ttaaagaaga
     9421 tggagaaata tatagagttg gtggatgggg gtataatttt agtgatttgg gttctggata
     9481 tgatataggg agaaaattat taaagaaagt attgctttat tgtgatgaat gtcatgagta
     9541 tagtgattta tttagttgtg tcttggattt ctttggagca aatagttttg aacaacttgc
     9601 atatatgatt actgatataa acaataatgt tgaaatagca aacttagctt cattagtgat
     9661 tgattgtgcc gaacaaggtg ataaattggc tattgaaata ttaagagaat catcaactga
     9721 actatctaaa ttagctcaag taatattgag aaaaatatct aatccaatga aagtggataa
     9781 aagcgaaata aatatagttt tgtctggtgg tacattaaat aaaactgtat atgctcaaat
     9841 gttaatgaat aatttaaaaa cagaaaatac agataaaaat ttaaaattta tatctcaaga
     9901 aaatcagcca gtgtatgggg ggataagact tgctatggca ttagctaata ggagggtcaa
     9961 gcatggatag aaaaaaggta atagtacaaa tttttactgg aggttttaag gctaaagaaa
    10021 taaagtttaa tgatataaaa tgtaaactac tagaattatt ggactgtatt gatatagaaa
    10081 aggttatcat gggatggagt gtagatgaag aattatatgt agaaactaaa aaactattaa
    10141 ataaatataa tgttgaacta tatctttggt tgccatgttt ttctgaaata ggattacttg
    10201 aaaaaagtaa tttgctaatt gattatatgg ggaaggaagt caagggctat gctttacaag
    10261 aggatgaaaa ttttgagttt tattgcccaa ataataagga aaatataaaa aatataaaga
    10321 aaatatatag tacttatttt agtgatatta aatttgatgg aattttttta gataaaataa
    10381 ggtatggttc tttttcgaat aaactaaatg gtgtttttaa ttgtttttgt aaag
//
LOCUS       DAEMGG010000132        10431 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEMGG010000132 DAEMGG010000000
VERSION     DAEMGG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.133"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..1132
                     /locus_tag="KPK24_003170"
     CDS             92..1132
                     /locus_tag="KPK24_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG2300819.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMESVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            1271..1723
                     /gene="tsaE"
                     /locus_tag="KPK24_003171"
     CDS             1271..1723
                     /gene="tsaE"
                     /locus_tag="KPK24_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG2300820.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1723..2439
                     /gene="tsaB"
                     /locus_tag="KPK24_003172"
     CDS             1723..2439
                     /gene="tsaB"
                     /locus_tag="KPK24_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG2300821.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKITHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVDFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2429..2905
                     /gene="rimI"
                     /locus_tag="KPK24_003173"
     CDS             2429..2905
                     /gene="rimI"
                     /locus_tag="KPK24_003173"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG2300822.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2911..3927
                     /gene="tsaD"
                     /locus_tag="KPK24_003174"
     CDS             2911..3927
                     /gene="tsaD"
                     /locus_tag="KPK24_003174"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG2300823.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREILSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4224..6932
                     /gene="hpdB"
                     /locus_tag="KPK24_003175"
     CDS             4224..6932
                     /gene="hpdB"
                     /locus_tag="KPK24_003175"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG2300824.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6936..7193
                     /gene="hpdC"
                     /locus_tag="KPK24_003176"
     CDS             6936..7193
                     /gene="hpdC"
                     /locus_tag="KPK24_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG2300825.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7186..8136
                     /gene="hpdA"
                     /locus_tag="KPK24_003177"
     CDS             7186..8136
                     /gene="hpdA"
                     /locus_tag="KPK24_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG2300826.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            8452..9414
                     /locus_tag="KPK24_003178"
     CDS             8452..9414
                     /locus_tag="KPK24_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2300827.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(9513..10316)
                     /locus_tag="KPK24_003179"
     CDS             complement(9513..10316)
                     /locus_tag="KPK24_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2300828.1"
                     /translation="MMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLTYSLLIVLK
                     LLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPIKNIKLGSLLIIYFMISVIVP
                     TSFILILQGSQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFNKFGKKLG
                     VIILGICWSLWHLPLIFTLYTPETPILGLILRSIHIVGISIFLGYLYIKTKNIWFCAI
                     IHILNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT         4117 a         1071 c         1893 g         3350 t
ORIGIN      
        1 acaaaaaata aagttattat taaaaaacta taaagattaa taatcaaact tttaataata
       61 actaatttaa taatgatagg ggtgtatatt catgcattta tctaaaaaaa taaaaaaatt
      121 atttttagat gataaagggt attattttaa tagtaattgg aaagatattc atattggtat
      181 ttatgataca aaattaggag attctttaaa gtatatcact aatgaatata ttgaaaatat
      241 ggaaagtgta aaatacgatt atcgtgaatt aaaaaatata gaaaatcaaa ctgatggtat
      301 gtgtatttta gcaataaggc agaattacaa ggcattagag tatgtaaaaa atcaaaccga
      361 agaattatgt ttagaagcaa taaagtataa ttataaagca cttgaatatg taaaagaaca
      421 aacagaatac ttgtgtcttg aagctataaa aaaagattgt aatgcactta agtatgtacg
      481 aaataaaaca gaagggctta ttataaaagc aataagtcat tcttccaata tagatgttgt
      541 aagtatacta aaggctttag agactcaaac aagaaggata tgtttagaag caattaaaaa
      601 agatggcagg tgtcttgcat acgttagaga acagagtgaa gaattatgta tagaggccat
      661 taagcaaaat tataaagcat taaaatatgt aaaaaatcaa acggaaaaaa tgtgtataga
      721 atccgtaaga caaaatggta tggcattaca atatgtaaat aaacaaactg ataaaatatg
      781 tatagaagct gtcaaacaag atggaagaag tatacaattt gtcaacaata agacagaaga
      841 aatatgtata aatgctataa gatatttaaa taaaaaatat aatattaaag atgtactaag
      901 ttacatagat aagtatacag aagatatatg tatagagatt gtaagacaaa atggaaaaat
      961 gcttatgtat ataaaaaatc aaacagaaaa aatgtgtatt gaagcagtaa aagagaacta
     1021 taagtctctt aagtatgtaa aagagcaatc tgagagaata tgtaaagaag cattgaagca
     1081 aaaccataaa gcaaaagagt atgtaaaaat tgctattgat gattgtatat aaggtgtaga
     1141 tacagccatc aatagtgaaa gataaaaaaa gataaagaca agtactttat aataatctag
     1201 gcttatacta taattaatta agcagtgata taaattcaca tgacgattat atcaaaatgg
     1261 agatgaattg atggcaaaaa tatatttaga aaatgaaaat aaaactagag aaataggata
     1321 taaattaggt aaacttttaa aagaaggcag tgttatctgt ttagtaggag atttaggagc
     1381 tggtaaaact acaatgacac aaagtttggc agattcatta gggatagaag attatataac
     1441 aagtcctact tttactataa taaatgaata tgaaggaaaa atacctttgt atcattttga
     1501 tgtttataga ataggtagta gtgacgaaat gtatgatatt ggttatgatg aatatgtaaa
     1561 ttcaaatgga atctgtataa tagaatgggc taatctaata gaagatatac ttccaaaaga
     1621 atatttgaat atagaattga gatataaaga tgaaggaaga gaaatgatat taactccaaa
     1681 aggagagttt tataaagaga tagttgagga gttgattaaa taatgaaaat actaggtatg
     1741 gatacgtctt ctatggcagc aagtgtagca gttgttgaag atgataactt aatatgtgag
     1801 tttacagtaa acaacaaaat aactcattca caaaagctta tgccaatgat agaaaatatg
     1861 ttgagtatga gtgatttaag tataaaagat atggatttat tagctgtatg tataggtcca
     1921 ggttcgttta caggacttag aataggtatg gctacagtaa aagctatggc ccatgtaaat
     1981 aatataccaa taatagcagt aaattctttg gaatctctag caaataacat aaatttctgt
     2041 gatagaaaga tatgttgtat attagatgcg cagaaaaatc aagtttattc agctaaatac
     2101 aagtttgaaa atggagagat gatagaatta gatggtgttg atgttgtaga ttttgatgct
     2161 ttagtagatg aaattgtatc aactaatgaa gaatttataa tagttggaga agctgtatac
     2221 aaatataaag acaagttaga gaatataaaa aatataaaaa taccttcacc agctaataat
     2281 gtatctaacg caggaagctt atgctcttta gcattaaata aatataataa aaatatagat
     2341 gtacatactt gttatacgat aaatcctatg tatatcagaa aatctcaagc tgaagtacaa
     2401 tatgatgaaa aaatgaagag gctaaataat ggaagataat acaaaactaa tagtagatga
     2461 tataaaaata gaagaaatga ctacagaaga tatagatgaa gtttttgaag tagaaaaaaa
     2521 ttgttttgaa gattattggt caaaagagtc ttttagaaaa gagttgtcta atgaagtagc
     2581 aaggtatata gtagctaagt taaatggaaa aattgtcgga tatgtgggaa tttggcttat
     2641 acttgatgaa gggcatataa ataatgtggc tgttcatagt gactatagag ggaaaaaaat
     2701 aggggacaag ttgataaaag gcatagttga tttatgtaaa gataataata ttgcatctat
     2761 gacattagaa gttagagcat caaataaaat agcacaaaat ttatatagaa aatatggatt
     2821 taaaatgggt ggaataagaa aagaatacta cagtgataat aaagaagatg caattataat
     2881 gtggaaccaa ttaaaagagg tgtagagttt atgagtgata taataacatt agctatagag
     2941 agtagttgtg acgaaacagc tgcatcagtt ttaaaaaatg gtagagaaat actttcaaac
     3001 ataatctcta ctcaaatcga gacacataaa aaatttggtg gtgtagttcc agaagtggcc
     3061 tcaagaaaac atgtggaaaa tattgatata gttgttcaag aagctttaga caaagcaaat
     3121 ataggtttta atgatataga ccatatcgca gttacatatg gacctgggct agtaggtgct
     3181 ttattggttg gtctatctta tgctaaagca cttgcatata ctttgaatat tccacttgta
     3241 ggagtaaatc atatagaagg gcatctaagt gcaaactata tagagcataa ggatttaaaa
     3301 cctcctttta taacattaat agtatctgga ggtcatactc acttggttga agtaaaagat
     3361 tatggcaagt atgaaatttt aggaaaaaca agagatgatg catctggaga agcatttgat
     3421 aagatttcaa gagctatgaa tttaggctat ccaggaggtc ctattattga taatctggcc
     3481 aagaatggaa ataaacatgc tatagaattt ccaagagcct atttagaaga agatagctat
     3541 gattttagtt ttagtggatt aaaatcttct gtattaaatt atctaaatgg aaaaagaatg
     3601 aaaaatgaag aaatagtagt tgaagatgta gcagcatctt tccaagaggc tgtagtagag
     3661 gttttatcta ctaaagcact aaaagcagta aaagacaaag gatacaatat cataactctt
     3721 tctggtgggg ttgcttctaa ttcggggctt agagctaaaa taacagagct tgctaaagac
     3781 aatggtatta ctgttaagta tcctcctcta attttatgca cagataatgc agctatgata
     3841 gggtgtgcag gatattataa ttttataaat gggaaaacgc atgatatgag tttaaatgca
     3901 gttccaaatt taaagataaa tcagtagagt tagatgaatt tgtaaatata aaagtattgt
     3961 ttcaaaaaag taagtagaag tgaaattaaa tataataaaa ataaatatag tatatattaa
     4021 aacttgaagt aaaactattt ataatcttta tattccaatg aattgcaaca aattttagga
     4081 tatacagatt gtctttggct tagatttgct tcaagtttat ttttttctat agaaagatta
     4141 ttttaaaaag tgggtaatat accctttttt ttcattctta aaaagtttta tactaattat
     4201 agaaagatta aggaggggaa gaaatgagtc aaagtaaaga agacaaaata agaagtattt
     4261 tggaagcaaa aaatattaaa tcaaattttc aaaataaaga aaatttaagt gaatttaatg
     4321 aaaaaaaagc aagtaaaaga gcagaagact tattagatgt atattataat acattatcta
     4381 ctgctgatat ggaatttcca tattggtata atagagaata tagaaaatca gatggtgata
     4441 taccagtagt tagaagagct aaagctctta aagctgcttt ttcacatatg acaccaaata
     4501 taatacctgg tgagaaaata gtaatgcaaa agacacgcca ttatagaggt tcttttccaa
     4561 tgccatgggt aagtgaaagc ttctttgttg ctcaaggaga acaaatgaga gaagaggcaa
     4621 agaaattagc cagtaacaca gctgatgaac ttacaaaatt tgggtctggt ggaggaaatg
     4681 ttactgaaag ttttggaaat gtcgtttcaa tagctggtaa attcggaatg agaaaagaag
     4741 aagtacctgt acttgtaaaa atggcaaagg aatgggttgg aaagtctgtt gaagatttag
     4801 gttttcacta tgaaaaaatg atgccagatt atgatttaaa agaaaaccta atgagtacac
     4861 ttatatgcat gtttgattca gggtatacac ttcctcaagg tagagaagtt ataaattatt
     4921 tttatccttt aaattatgga ttagatggaa ttatagaaat ggcaaaagaa tgtaaaaagg
     4981 cagttgcagg aaatgcatct ggagatgggc ttattggtat ggaccgtcta tatttttatg
     5041 aggctgttat tcaagttatt gaaggattac aaacttggat tttaaattat gcaaaacatg
     5101 caaaatattt agaaagtata gagacagatt tagaagctaa aaaagaatac tcagatttag
     5161 tggagatatt agaacatatt gcacataaac aacctcgtac atttagggaa gctcttcagt
     5221 tgacttatac aattcatata gcatcagtaa atgaggatgc tatatctggt atgtctatag
     5281 gacgttttgg tcaaatttta tatccttggt atgaacaaga tatagaaaaa ggactaatta
     5341 caaaagaaga ggttatcgaa ttattagagc tttatagaat aaaaatcact tgtattgact
     5401 gttttgcttc agcgggcgta aatggaggag tactatcagg caatacattt aatactttat
     5461 ctataggtgg attaaaagaa gatggttcta caggagctaa tgaactagaa gagttattat
     5521 tagaagcaag tatgagatgt agaactcctc aacctagttt aactatgcta tatgatgaaa
     5581 agcttccaga agatttctta atgaaagctg ctgaatgtac aaagcttggt tctggttatc
     5641 cagcatgggt aaacaactca aatggaacaa catttatgat gaaacaattt gctgatgaag
     5701 gtatgactgt tgaagaagca agagcatttg cattaggtgg atgtttggaa acgtctccag
     5761 ggtgttggaa acagttaact ttaaacggaa aaacatactc tattgcaggt ggagcaggtc
     5821 aatctgctgg ctctggagtt cactttatag ctaatccaaa aattttagaa cttgttctta
     5881 tgaatggtaa agactatcgt atgaatatac aagtatttga gcctcataat aagccattag
     5941 atacttatga agaagtcata gaagtattta aagattatta taaacaagca ataaatgttt
     6001 tagaaagagc aaataacatt gagttagata tatggagaaa atttgatact tctataataa
     6061 attcactact aaagccagac tgtttagaca aaggtcaaca tataggaaat atgggatatc
     6121 gttataatgc aacattaaac gtagaaactt gtggtactgt tactatggtc aattcatttg
     6181 cagcacttaa gaaacttgta tatgatgaca aagcatttac aatagaggaa atgaaggatg
     6241 ccatattaaa taattttgga tttaaagatg cattagaagt aggaaattat tctatggcag
     6301 accaagtaaa agtagataag actggtaaat atgatgctat ttataaggcc tgtcttgatg
     6361 cacctaaata tggaaataat gatttgtatg cagataatat tcttaaaaac tatgaggtat
     6421 ggttatcaaa agtatgtgaa gaagcacaat ctttatatgc taagaagatg tacccttgcc
     6481 aaatatctgt ttctacacat gggccacagg gagctgctac cttggctact ccagatggta
     6541 gactaagtgg aacaacatat tcagatggtt cagtctctgc atatgcagga acagataaaa
     6601 atggagtata tgcattattt gaatctgcaa ctatttggga ccaagcagta gttcaaaatt
     6661 ctcagatgaa tttaaaactt catccaacta ctataaaagg acaacaagga actaaaaaat
     6721 tattagattt aacaagaagt tatttaagaa aaggtgggtt ccatattcaa tataatgttg
     6781 tagattcaga aacattaaaa gatgcacaaa aaaatcctga caattatcgt caattaatgg
     6841 tacgtgttgc tgggtttact caatattggt gtgaattagg taaaccaata caagatgaag
     6901 taattgctag aacagagtat gaaggggtgt aaaatatgag aaagcatagt gattgtatga
     6961 atttttgtgc agtagatgca accaaaggaa tttgtagatt atcaaaacaa atgattaatt
     7021 tagatgatgc agcatgtcca gagataaaag taatgccaaa atgtaaaaat tgtaaaaatt
     7081 ttgttgaagc taatgatgaa ggtataggca aatgtgttgg cctagagaaa gaagactggg
     7141 tatattcaac attgaatgca attacttgtg aaggacatgt gtttaatgag tagtcaaaag
     7201 caattagaag gcatgatttt tgatgtacaa agcttttcag ttcatgatgg tccaggttgt
     7261 agaacaactg tatttttaaa tggatgtcca ctgagttgta aatggtgtgc aaatccagaa
     7321 agttggactg taagaccaca tatgatgttt agtgaattat cttgtcaata tgaaaatgga
     7381 tgtactgtat gtcatggtaa gtgtaaaaat ggtgcattga gctttaatct tgataataag
     7441 ccagttatag attggaacat ctgtaaggac tgtgaaagtt ttgaatgcgt caactcatgt
     7501 tattataatg cgtttaaatt atgtgcaaaa ccttacactg tagatgaact tgtgcaggta
     7561 attaaacgtg attctaacaa ttggagaagt aatggaggag taacatttag tggaggtgag
     7621 ccactacttc agcatgaatt tttacatgaa gtattattaa agtgtcatga ggtaaatata
     7681 cacactgcca ttgaaacgag tgcatgtgta tcaaatgaag tttttaataa aatatttaac
     7741 gatattgatt ttgcatttat tgatataaag catatggata gagaaaaaca caaagaacaa
     7801 acaggtgtgt ataatgattt aatattagaa aatatatcaa accttgcaaa ttctgattgg
     7861 aatggaagat tagtacttag agtaccagtt atctctgggt ttaatgatag tgttgaaaat
     7921 ataagtgata ttatatcttt tatgcacaaa aataatttgg tagaaattaa tctattacca
     7981 tttcacagat taggagaatc aaaatggatt caattaggca aggaatatga gtattctgat
     8041 aagggtgatg ttgatgaagg tcatcttgaa gaattacaag atatattttt ggataatggt
     8101 atagcttgtt atgttggtca tgagacagct ttctaaaatt aaatacaagt tttaattaaa
     8161 aaaaggatgt tacactaaaa gctagagtta catccttttt tatgtggttt ttatgtagaa
     8221 ataattaagt aaagttttat atgttttata tattgtttat attgctttat gtccataaat
     8281 attgctttaa atatacttta tatatttttt atagtttaaa ttacagttag agaatatatt
     8341 acgcttactt cattgaagca gaagttttgt ggtaaaatac tactatgaaa aaatttaata
     8401 gaatatattt tagaaacaac taaaaataat agttaggagg aagacatcta tatgaacaaa
     8461 tcatggagca acttgattat taagaataaa gacaaaaaag tacgttcagg gtggattatt
     8521 atagctgtta tggctacctt ttacatactt cagtattatt taagcatgat acttgtggaa
     8581 ataataagaa aaatcctggt tgctacaggt gacattaatt taaaaacaaa ctatttttca
     8641 gaatatgcta attggttaaa tgatgttggg ttacagattg tatttcaaat tctaacagat
     8701 tcacttatga ttattattcc tataattgta tggagattta ttatgaaaaa tcctattagt
     8761 gaaatgggat tacattctta tagaattaag aaaaaagaag gatatgtagg aatgttatta
     8821 ggaatattta attgtacagt aatctttatt ataacaataa caattggtgg tggtcatgtt
     8881 acttcatggg taccaaaaat ttcaccttta acagtttctt ggatacttgt ctttatttta
     8941 gttgcttttg cagaagaaat tttaaatcgt ggttttttta tggcagtttt acgaagatgt
     9001 aaaaatattt attttataat gattgttcca tctgttattt ttgggttgat tcatatatgg
     9061 aatcctgatg ttactttttt atctgtaatt aatattataa ttattgggat tcttttttca
     9121 tatatgttta taaaatcgtc gaatatatgg atgtgtatag gttatcattt tacatggaat
     9181 gtatttcagg ggattatata tggtatgcct gtaagtggac ttcaggttcc agggattata
     9241 actacacaaa tcactcatgg taatctatta aatggtggta tgtttggtat tgaaggtgga
     9301 attttgacta cttttgttaa tctacttagt tttatatttg tatggtatta ctatagagat
     9361 tctaagtatg attttgttag tgataaagta aatgtgacca atatcaagaa ttagttgatt
     9421 ataataaatt ttaataatat cttctaagct taaagtattc tgtaggattg ttggaaattt
     9481 acaaaaatat aaaaataact tggatattct atttactcca agttattttt atattctttt
     9541 gtaaatagaa atggaacata aaaaattaaa acaaccatag atactattag tatatcatgg
     9601 taagtgatag ttgattcttt agaatttgtg ataagaaaag cactattatt taaaatatga
     9661 attatagcac aaaaccagat gttcttagtt tttatataaa gatatcctaa aaatattgaa
     9721 attccaacta tatgtatcga acgtaatatt aatcctaata ttggtgtttc aggtgtatat
     9781 aatgtaaata ttagtggtaa gtgccacaaa ctccaacaaa tacctaatat tataactcct
     9841 agttttttac caaatttatt aaaaaaaata ttctgtaaaa aacctctcca ggcatattct
     9901 tcactaaaaa aaatataaat agtagttaat gatgttattg gagtaaatat aaattttatg
     9961 ataccactat aattaggttg agacccttgt agtattaata taaatgatgt tggaacaatt
    10021 acagaaatca taaagtaaat aattaacaaa gaacctagtt taatattttt tataggatat
    10081 aaattttttt tatttgatga atatattata attgataaac tacttataaa agttaatatt
    10141 aggtttataa ttataaattc attattattt attagattta ataacttaag aacaattaat
    10201 aacgaataag taagaaaaaa tagtatgatt agtgaataaa attcatacat atcattattt
    10261 aatccttcag tataaaattt tgcaatagca actgaagata gaggtaatat catcatgaat
    10321 gatgcaaagt tttctggatc aataaattta ttataataca ataataaacc taatgaataa
    10381 gttaaaccaa agtttataaa gaaaaatata ataatttgta ctttttttct t
//
LOCUS       DAEMGG010000133        10370 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEMGG010000133 DAEMGG010000000
VERSION     DAEMGG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.134"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            181..1413
                     /locus_tag="KPK24_003180"
     CDS             181..1413
                     /locus_tag="KPK24_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG2300829.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            1427..2233
                     /locus_tag="KPK24_003181"
     CDS             1427..2233
                     /locus_tag="KPK24_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG2300830.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2345..3214)
                     /locus_tag="KPK24_003182"
     CDS             complement(2345..3214)
                     /locus_tag="KPK24_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2300831.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3586..5013
                     /locus_tag="KPK24_003183"
     CDS             3586..5013
                     /locus_tag="KPK24_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG2300832.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIGSVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5090..5359)
                     /locus_tag="KPK24_003184"
     CDS             complement(5090..5359)
                     /locus_tag="KPK24_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300833.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMRGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5362..6804)
                     /gene="nhaC"
                     /locus_tag="KPK24_003185"
     CDS             complement(5362..6804)
                     /gene="nhaC"
                     /locus_tag="KPK24_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG2300834.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6829..7995)
                     /locus_tag="KPK24_003186"
     CDS             complement(6829..7995)
                     /locus_tag="KPK24_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2300835.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKYYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(8220..10340)
                     /locus_tag="KPK24_003187"
     CDS             complement(8220..10340)
                     /locus_tag="KPK24_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2300836.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSNNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
BASE COUNT         3716 a         1401 c         1528 g         3725 t
ORIGIN      
        1 taataaattt ggactattta tctatttata acttaaatta tatcaaattg ataacaaact
       61 agatattata cttttttgta gaatagaatt acaattatat aaagaaaaat tctattaata
      121 gtgaaaaaaa agtagaaaaa agtagaaatt attcttttat aaagaaaggg agggataata
      181 ttgcaagaga gagaagaatt agttttaaat ccagttccaa aagaggaaag agttggatgg
      241 ttagctccat tgtttaatat gcttggttct aacatagcta tatctgaact aatggttgga
      301 ggaactttaa ttttaggaat gactctttct aatatgatta ttacatcaat tatagggaac
      361 ttaatattag ttgcaattat tatgatacaa ggttatatag gttacaaaga agggttaaat
      421 acgtatgtat tagctaaggg ggcatttgga gagataggag gaaagtatct tatatctcta
      481 ttacttggta taactagttt tggatggttt ggtgtacaag caggggttgc aggactatca
      541 gtacaaaaga tatttccaag tgtaaatcta acattagtta cagttatact tggattattt
      601 atggttgttt ttgcactgta tggctttaaa gctatggcta aatttaatta cttagttata
      661 ccaccactta tgatattaat ggtgtgggga gtttttaaag ctttttctac ttatggagtt
      721 gaagctattt ataattatac tcctcaaaca actatgagta tggttgaagg tcttaatatt
      781 gtagtaggtc ttgttatagt tggtgcaatt atatcacctg accagttaag atatacgaga
      841 cgagtaaaag atatttggat tatcagtttt ttaggtctag ggattatttc attattccaa
      901 caagttgcag ctggtgtaat gtctatggga gcacctactt gggatataac agaagtattg
      961 gctaatttag gttttggatg ggtagcgttt gttattttaa tacttgcatc ttggtctaca
     1021 aacgtatcaa atgcttattc tggtggatta gcattaaaaa ctatttttcc aaatgtaaaa
     1081 agaaatgttt tgactttagt tgcaggtcta ataggtacaa taatagcagc tactggtata
     1141 atatttaaat ttcagtcatt tttatctttc ttgggaatcg cagttcctgc aatagctgga
     1201 attatgtggt gtgagtatta ttttatacaa ggaagaactt ataagcatag agaaggaata
     1261 aattggattg cagtaataag ctggttaata ggttttgcag cgtcgtatta tagttcaaaa
     1321 ataaatttct taatacctcc gataaatgga attgtagtga gtatggttat ttattatata
     1381 ttaatgaaat gttttggaat caaagataaa taaagggggc aattacatgg gtcgtaaggt
     1441 gaaaataggt ataattcaac aacacagtgt acttggagat gttaaaaaaa atatagaaaa
     1501 ggcagtagag atgatagatg acttaggaaa acaaggggca gatataattt gtttaccaga
     1561 actatttgct acaggatata atttagagtc tttaggtgga gttaaaacat tagaattaat
     1621 aagagaacat aataagtaca tagaggaatc aatgtcagaa gctgctaaaa gaaataatgt
     1681 atacctgata tctccatatg gaaccttaga aaaagggtct acacatgttt ataattcagc
     1741 agttatattt gatagaaaag gtaagattat gggagaatac tgtaaaaatc atctgtggtc
     1801 attagaagca gtatacttta aaggagggga aaaagtagag gtatatgatg ctgattttgg
     1861 tagatttggt gtaatgatat gttatgatgc aggttttcca gaagtatcca gagaattgac
     1921 attaaaaggt tctgaaataa tatttatacc atctgcatgg agaatacaag atgaagatat
     1981 gtgggattta aatgtatctc aaagagcttt agagaacact gtatatacag taggtgtaaa
     2041 tttagtaagt aatgactcta atttaatact atttggaaag agtaaaatat gcaatcctag
     2101 aggtactgta ataactcaat tagatacata cagggaaaaa tatgtattat cagagattga
     2161 tttagatgaa ataaaaaagt atagggaaga tatcccctat ttgaaagaca gaaaaagtag
     2221 ctataacata taattctata ctataataca agttcaatat aaaatgataa aaagaggatg
     2281 tatcaatatt aagattaaat ttattatact ttgatatatc ctctttaata ttatcaactt
     2341 tgatttaaat atttttaaaa tagttgataa gaaagttttt gaacaacaac acagctttag
     2401 attcatagtt ttcgctaaac catgaaagat ttaaatatct tttaaattca atatctttga
     2461 tatgtaacaa cttaatctca gggtcttgag ataatcccca tgattttcca catataaatc
     2521 caactccttg aagtgatttt ataagagcat aaattgtata tggtgaatca gactcaaatg
     2581 ctattttggg tttgaaatta gctgattcgc acaggatatc aattacctct ctataattct
     2641 cacccttcgt tataactata aagttttcat ctgaaacttc gcttagatat acttcatcct
     2701 ttaatgataa aggatggttt attgaaactc ctaatagtat ttcttcgcat gttaaagtta
     2761 tgctattttc tgagtttagc tttgtaaatg aagaagatat gtacaaatca aaatcacttt
     2821 ttttataaga tgatggcaaa gtatgtgaca cattaaaagt aatattggga tatagttttc
     2881 tgaaattact caaaagtgat ggtaacacat tagccgctgc aggagataat atctttactt
     2941 ctcctgaatt ttctaaagaa gtgtcttgta actccttttt tgaactttct actaaatcta
     3001 aaatgctatc tactcttttt agaaacaatc ttccatactc atttaaaaca atatatcttc
     3061 cttttctgtc aaataactga actccaagtt ctttttctaa ggaagatata gttttgctta
     3121 aagctggctg tgctatattt aacttgtgtg atgcttttgt tatgtgctcc attctagcaa
     3181 cagtttgaaa atacttaaga tgcaataaat ccatagtata aaatacacct cttttaatat
     3241 gacttgagtt atatcaattt tataatcaac tccacattat acattataat ataaagttta
     3301 tataataaac tacaatatat taaaaagtta tttaaattgt tatttatatt ttgtacataa
     3361 tctaattaaa aaaagtaact ttaaatgatt aactaaggaa atgttgttac aaactttagt
     3421 tatataaaaa attcatttga tgagcaaagt actaatatca agtaagaata aaagcttatt
     3481 agataagaaa aataatattt tataataatg atgttactta aattattatg ttatactatt
     3541 ttaaatagga aaaatttaga tactataatt ttagggggag tcaagatgaa attaactaaa
     3601 tcaatatcat caatgctgtg cttatctcta gttttaagct tatcaccagt agcatatgca
     3661 aatgaaataa gtagaacaac agttgagaat actttagtag gtcaggatag atatcaaact
     3721 gctgttgaag taagtaaaaa aggatggagt agtgcaaatg aggctgtagt tgtaaatagt
     3781 gaatctattg tagatgcatt agctgtaaca ccatttgcta aattaaaaaa tgctcctata
     3841 ttattgacag aaaaaaataa tctaaattca caaactgaga aagaattaaa aagattagga
     3901 gttaaaaaag tttatataat tggtctatct agttctgtat caaatgatgt gcaaaaacag
     3961 ttagaatcta ataatttaac tactgataga gttggtggta gtgatagata ccaaacagct
     4021 ttgagtatag ctaaaaaaat agaaggatta aaagatatat cagaaatagc tgtagtaaat
     4081 gggtacacag gtcttgcaga tgcagttagt ataggttctg tagctgctac taatggaatg
     4141 gttatacttc cagtatctga tgcatctggt atatctagct ttaaagattt tatagaatct
     4201 aagaatataa ataaatcata cataataggc tctactaatg ctgtatcaga taaaataaaa
     4261 caaagtttac ctaattcaga aagaataggt ggagcagata gaaatgaaac taatggtaaa
     4321 gtaatagaaa agttttatac atctaataaa ttaaacaatg tatttgtggc taaggatgga
     4381 atgaaaaatc aaaatcaact tatagacgct ctagcagtag gagtactagc atcaaaagaa
     4441 aattctcctg tactaatcgt aggagaacaa ttaagtgcaa cacaaaagag tgtattttcg
     4501 aataaaaatg ctacctctat aactcaagtt ggtagtggag gaaatgaaaa tgcatttaaa
     4561 gaattaaaag atttacaggc aaatagtaac aataagagag taatgtatgt aactaataca
     4621 gataaggtaa atataagaaa tgatgctaca atagaagctt ctgttatagg atatctaaat
     4681 aatggtgatg aagtagaagt tttagatgtg ttaaaaactg gatgggtaaa aataaaatac
     4741 aatgaaggta ttggatatgt tagtgggtct tatttgacaa ataataagcc tgataatagt
     4801 aatgaaaata taaaaataaa gtatgtaaaa gaaaaagatg gtctaaatgt taggaaagga
     4861 ccaagtacag aagatgaaaa aataggtcac ctttcatatg gaagcaaagt agaaacaata
     4921 gaaatgtttg ctactggatg ggtaaaaata aagtacaatg gtggatatgg ttatgtaagt
     4981 aatgattact tatctgatac accagttatt taattataag taacaataaa aaaactgctt
     5041 taatttgtaa agcagttttt ttataaaaat ttaagtctaa taactatttc tattttgata
     5101 ccttaatcct gggatttatt attatggata ttatcaacat acaaacactc caaatccagt
     5161 gtctaattcc aaacttagat attaataaat tcagtcctgc tatggtaaat aagcagtatt
     5221 taattacaat taaaagtgta tttatagaaa gccccctcat agaaagtatc ctaaatccaa
     5281 taatagacat tctaagtcct ataaatgcca ataatatacc tgcaatatcc tgaattaagt
     5341 aatagtattc aaatttcata ttcaatcaac tcttctctga gcagtatcaa atatttctaa
     5401 ctcttcaatt tcatcatctg tgtatgtaag catagtaaaa ccagttacac cataaataaa
     5461 tgaaattata ggtgtaatcc aacttaagaa acaatatgga ataaattcta gtggacttac
     5521 accaagagtc tgagcacaga aaacagcatt tgaattccat ggtattaatg gaccaccaag
     5581 tgtagctgta tcttctataa ttctagatag attttgtggt tttaatttaa attctttgta
     5641 tattggagac attagagttc cgacaaatac agaagtgaac atcattgatg aactaaatgc
     5701 atttgcaaag tagcttacta taaaagtaga cccaaccaat ttagttcttg attttcctat
     5761 tttagttata agaggttgaa gcagtacttc taagaatcca gttttttgga gaataccagc
     5821 aataacgaaa actacaaata ctagtaaaac ggtttcagcc atactcatta ttccaccacg
     5881 atttaatagt ttgtcggcat agacaaatcc agtatctata gtaaatcctg atagcatgta
     5941 atttaaaaca tcacctattt tttctccttc ttgaaatact gctataataa gtcccatgat
     6001 agctgaactt agtatagata taatgggtgg cttttgtagc aataaaagta ataataaaaa
     6061 tattattgga atcattgcta ctaaaccaat atgaaagttt gaatttaata catccttgat
     6121 ttgatttatt tgaacatagt ctatagtatt atttgaatac ttaaatccaa ttactgtata
     6181 cagaactata catattacgt atgcaggtcc tgtggtataa aacatatgct tcatatgtgt
     6241 aattacatct gtcttacaaa ctgctgcagc taagtttgta gaatctgata gaggtgacat
     6301 cttatctcca aagaaagcac cagaaatgac tgcaccagct gttaaaccaa gaggaatacc
     6361 catactagta ccaatactca tcattgctat accagaagtt ccaagagtcc cccaagatgt
     6421 accagtagca acagaagtta atgaacatac gattaatgtt gtaagcagaa aatatttagg
     6481 ggttattatt gttagtccac tatatactac tgttggtatt gtaccagcag ctatccaagc
     6541 tgctattagt acaccaacag aaagtataat taagtttgat tgaaaggcat ctttgccaac
     6601 ttcaaatcca aaagcttcta tttcatcatt agtataacca agtttcatgg ctgcaggaac
     6661 tactataagc catgaaagta aaaacattgt agttatagaa gcattaaata caactatacc
     6721 aactgatgtt agagctatta ttgcaagcat tactaacaag gaatatctaa atgaaggtat
     6781 ttttctttct gtattcattt tcatagtgca aattctcctt aaatattttt agttaattgc
     6841 attttttatt cttataagag cttcttcaag catacttctt ggacaagcta agtttattct
     6901 ttgataccca cttcctccaa tgccaaagct gtttccttga tttagagcaa ctttaccttt
     6961 ttgtacaagt atactttcta gttcctcatc acttaatcca agtgcactaa agtctaccca
     7021 taataaataa gtaccttctg gttttctaac tttaagtttt ggcatatttt catttatata
     7081 ttttatagca aaatctatat tagattctaa atattctaaa aatgattcta gccaagattc
     7141 accattatta tatgaagctt cagttgcaac taagctaaag caattatttc tctttatatc
     7201 aattctggta aaggcatcat ctaacaattt atagtatttt tcatcaggta gtattacata
     7261 tgatgattga agaccagcta tattaaaagt tttagttgga gccatacaag taattgtatt
     7321 tttttcaaat tctttagaaa tagatgccat aggaatatgc ttatgttttt ttaatataat
     7381 gtcactatgt atttcatcag atatgatttt tacattatgc ttaagacaaa tatctcctag
     7441 tttttttaat tcatcttttg tccatactct accaactgga ttatgaggat tacaaagtat
     7501 gaataatttt acatctttaa tcttgttttc aatgtcttca taatccatta tgtagtttcc
     7561 attttctaat ttttgtaatg ggcttataat tagttctcta ttgttatttt ttacaacact
     7621 attaaaaggg ctgtaaactg gttcttgtat cattatttta tcatttgctt ttgtgagttc
     7681 atttattaaa agacttatag ctgggataac acctggacta taaattaacc actcactttt
     7741 tattttccag ttatgtctcc tatctagcca gtttactata gattcattgt aagaatctgg
     7801 tcttgttgtg tagccataaa tctcttgttc tagtctattt tttaaagaat caattataca
     7861 aggtgcagcc ttaaaatcca tgtcagccac ccacatagga agtaaatcat tagttccata
     7921 ctttttttcc atttctgacc atttagatga aaaattattg cttctatcaa caatctcatt
     7981 aaaattataa ttcattgtaa atacctccag taattttaat tttatgttaa taatattaag
     8041 caaacaacgt gccaatattg ataataaaaa atatttattc acaagttaat atttaatatt
     8101 aagtaatttc agagcataga taagataaaa atattaatta tacttaaaac tttgtggaaa
     8161 aggttatttc taataaattt ttaaatatat gaaaatgtaa ttaaataggt taaataggtt
     8221 taactgttta acttgttaga cctatttata ttttttagaa actctactcc ctttgatgtt
     8281 ataattgaac ctccacgacc aacaagtatg tctacaagcc caaagtcttg tagttctaaa
     8341 agcatatttc taatctgttg ttctgtcaaa aatctatttt cctctaaagc tattttacat
     8401 aagctttttc tacctattct ttcttttaaa tcataagcct tttttaaatt atccaatata
     8461 aatatgtatt catctagtga ccttttaaaa ttatagtcgt atttcatgat gtttttgtct
     8521 ttgccaatgt tgaattgata cttttttata ttattactta ctttattaca aacggttcta
     8581 ctataatttg aatctatagt atctaatata tattcaggta aatcacatat ttctataata
     8641 tctttttcca agtaacagaa atattctcct aagtttctta attctcttac atttccttcc
     8701 catctataca ttttaaagat ttcttttact tcctcactta gaataaaatt acatggaata
     8761 tcatatttta atgaaccaaa aatttcaaat atatcagcag gtctttccct aagtggatgt
     8821 attttaaggg gaagaacatt caacctaaaa tataaatctc ttctaaacat atttttagat
     8881 acaagttctt ttaaatttct attagtagct gcaattattc ttacatcaat gtcaatgaca
     8941 ttattagaac caatacgtcg tacttgtttt tcttgtatga ctctaagtaa tttagattga
     9001 gaatttaaat ccatttctcc aatttcgtct aaaaatatag ttccattgtt ggctaattca
     9061 aataaaccaa ttttcccacc ttttttagca ccagtaaaag ccccttcatc atagccaaat
     9121 aattcacttt gcagtagatt ttcttgaaaa gtactacaat ttactgcgac aaaaggacca
     9181 tcttttctcc tagaagcact atgaatggat tgagcaaata attctttacc agtaccactt
     9241 tcaccaatta taaggataga tgaatttgat tgagccattt tattagcaat tttttttgta
     9301 ttaatagttt gtatactaga acctaatata tcatcaaatg tgtatttact gatattacca
     9361 gagtttacta gttgtgctct taatttagct tgcttttttt ctaattgaga aaactttgtc
     9421 atctttagta taaatccatc aaatacattt aattgtatat gtttaatctc taaatttata
     9481 tcaatatgat ttattttaat caatttctca aaaaatggag ttttattggt caatatgctt
     9541 tgaaaattga tgtcttttat gcaatcacca acaaatgagt caatcatttc atttgcattt
     9601 atagacaaaa tttttctagc catatgatta tagaattgaa ttattccatc gttatttgtg
     9661 catataattc catcatcaat tatagatagt aacaaatcaa attgtctttc aaacttattt
     9721 gcatctaaca ataactcacc tgtactataa cttgtaggaa ttattttttc tacgtatttt
     9781 tttattaggt catcttttat tagatgtttt aatttggttt ttgtagcaat ttcaacaata
     9841 caacttaaat ctattattct gtattttaaa tctataactt cacaattttt aaacttaggc
     9901 aagatttttt cgccaggagt taggattaca gcatctgtgg gagtaaactc aatctctgga
     9961 tagcagggca ggatatttat attgtctatt cctaatctat gtattaaaga aattgtttct
    10021 attgccatat ctttacttaa gttatataca taaataattt tatctttttc taatttggat
    10081 atcatatcaa tactgttctt ttcaaatgtc aacttaggaa caatgatttg agcatcatag
    10141 tgagataatt ttactatttc atcatatttt aaataagcag ataggagtat aagtttttct
    10201 ttaaaaaact ttaaatttcc ttcttcaaat gagtataaat ttatctttag attatcacct
    10261 aagaattgtc ttatttgatt ttcataaatt tctccagcgt ctttttttaa acttacaata
    10321 gctaattcca tttgtttcat tttaacctct catacaaatt tgacttattt
//
LOCUS       DAEMGG010000134        10351 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEMGG010000134 DAEMGG010000000
VERSION     DAEMGG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.135"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1893)
                     /locus_tag="KPK24_003188"
     CDS             complement(<1..1893)
                     /locus_tag="KPK24_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2300837.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDF"
     gene            2127..2579
                     /locus_tag="KPK24_003189"
     CDS             2127..2579
                     /locus_tag="KPK24_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2300838.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTNEVKNASDASHLVKYITNKMKEE"
     gene            2582..2890
                     /locus_tag="KPK24_003190"
     CDS             2582..2890
                     /locus_tag="KPK24_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG2300839.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            3004..4110
                     /locus_tag="KPK24_003191"
     CDS             3004..4110
                     /locus_tag="KPK24_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG2300840.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            4185..6863
                     /gene="mngB"
                     /locus_tag="KPK24_003192"
     CDS             4185..6863
                     /gene="mngB"
                     /locus_tag="KPK24_003192"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG2300841.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(7180..9513)
                     /locus_tag="KPK24_003193"
     CDS             complement(7180..9513)
                     /locus_tag="KPK24_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2300842.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(9488..10204)
                     /locus_tag="KPK24_003194"
     CDS             complement(9488..10204)
                     /locus_tag="KPK24_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300843.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         3850 a         1459 c         1350 g         3692 t
ORIGIN      
        1 aaaatcatta caattagata tactatctct aatatcattg tcatgaatta tctttaaaag
       61 gatatcataa atggagttta tttttatata atcatcttta tttatagcca acatcatgat
      121 taatttaaca gtagaatctt cactccaata tattggttca tccaatatgg aaatacaaat
      181 cttagttctt atagagctta attccatagg atgaggtata gctaaaaaat catctatatt
      241 ggtagaagat aaactttctc tgtaaaatac agattcagca aatgatggaa atacaatttc
      301 actttgagtt aatttgttgc acatcaactt tataagctca tctttatctt tgattttagg
      361 attaacaata aataaatttt tatcaaagaa attgtccaaa agattactat aaatatggga
      421 attattagtg attgacttag atatattctc tatatctttt tttaatagtt ttaaatccac
      481 taatacaata ggaatactat catgatttaa aggaattgtc gatattatta tatctacatc
      541 tgacaattca ctagctaaaa attgattaaa tgatagacat tgaagtatat ttattttatc
      601 atgaaaaact ttgtttaatt gaacctctaa taacctactt gaaccatacc ctgaaccaca
      661 tactaatact acgtttttac acttaatatt actttgaaaa aatctctcta ttccagcacc
      721 tatatgcagg gatacatatc caatttcatc ttctgttaaa gagtaaatac tatttttaaa
      781 aactttttct atagcattta aagttatttc aaatgctaaa ggataattac tctttattgt
      841 atttataagg ggattcacct tatttaaatt ataagattta gaattgagaa ttgatttaaa
      901 gtgcagtact aaatcatcaa ataatacact atcatttcta aggtcaaaag tatattgatt
      961 gttgattact tctaaaattt cttcaacata ctctataatt ttagtatcta ccctattgga
     1021 aacgttatcc aaaagagaac ttttagttat aaaatgatta tatagataaa ctctgtctgc
     1081 ctttgaaata attatagtat atttactctc aatataatta aatatatttt caacagttgt
     1141 attcgattga aaatctgtta aaacaacatc taaaacatta tctaagcagt aaccatcaag
     1201 tattctactt acagtaatag ctaaatggat aataaagttt tttagattaa aatctaggaa
     1261 attgatatta aatttattaa tttcacttaa cactaccctt tgaagctcta ttaaatctac
     1321 attattaaat aattctattt ctctatctgt aattccaatt atatagtttt caaaattcct
     1381 atttatcata ttattagaaa tacatcttcg aatatctatt tccttacctt caactttaac
     1441 tccataatat ggcttagaaa ctaatattaa attatacttt gacaaaatag gctttattct
     1501 ttttatgtca tttagtattg taaatttact cacatacaag ctattagcta aatcttctag
     1561 ttttatatat gtattgttat agagaatata ttttaaaata tattgatttc tttctatagg
     1621 agaatctggt atttcacttg aatccataat atcatcagat attaatgcta gatactgttg
     1681 gtataatgct aaattaagaa tatctatata atatccttct ccttttttta ttttaataat
     1741 agcaccattt ttctctaaag ttccatttat agaagtaata tcatttctta tagttctctc
     1801 actcactttt aaatgattac ttaaagtttt acaagtaatt ttatcatcac tttcatttaa
     1861 taatttaaac aacgtgagca atctcttgtg cataaatatc cccccttata gaatttcttc
     1921 taaattatac tatttacact catataatac aattactcta ataaaattgt attataataa
     1981 tttatttcca cttctatatg gaaattagtt attatcataa tataaatttc catgtcgaag
     2041 tggaaattag ttatatttaa tttaattagg ataatgatta caatataaat ataagttaag
     2101 acattatgaa tggagtggaa aaatatatgg tactacaaga aaaatatata tttatagata
     2161 aagacttaaa aagcaaagaa gaagtattaa attttattgc agatagtgct tatatactag
     2221 gtattacaac taataaagag gctcttttaa aagaccttct aaaaagagaa aatgaatttc
     2281 caacaggaat aaaagatgga tttgcgatac ctcatacaaa aagtgtaaat gtaaaggaag
     2341 cttccatact ctatattaaa acaactaacg agttagagtg gaaaacttta gatgatagta
     2401 aggttagata tgtatttagt ttgttagccc cacataaaaa tgaaggaaat atacatttga
     2461 aaactctatc taaattagcc acatgtttaa tggaggatga ttttacaaat gaagttaaaa
     2521 atgctagtga tgcaagtcat ttagtaaaat atataactaa taaaatgaag gaggaataat
     2581 tatgaaaata gtagcaataa catcatgtat agcaggatta gcacacacac ctatggcagc
     2641 aaaagcttta gaacaagctg catcaaaaat gggtcatgaa atcaaagtag aacaacaagg
     2701 tgctatggga cgagtaaata ctataacaaa agaagaagca caagatgcgg actttatact
     2761 tatagcatct gaccaaacta tatctggaat ggaaagattt gatggtaaaa aggtgataaa
     2821 agtcaaaata ggtatagcgt taaaaaaacc agaagctgtc ttaacaaagt gtatagaagc
     2881 tatttcttaa taaaaaaatc tcactctaat atagaatgag aaaaatcaaa aattaaaaaa
     2941 ttgtctaatg taattataac atattatcaa aaaatataac aaacttatat aggaggatta
     3001 aaaatgagta aaaaaaataa atctctttta agtgaactta agggacactt aatgacaggt
     3061 atctcatata tgttgccact tgtaataggc tcatctttag ttgtagctat cccaaagctt
     3121 acagctttag caatgggtat aacaagttta gatgcttata aaaatgtatc tggtttttat
     3181 catattcttt acttaatgga acaagttggt tggactggca taggtctatt aaatacggta
     3241 ttagcaggat ttatagcatt ttcaataggt gagaaaccag ctatgggtgc aggttttatt
     3301 ggtgggctca tagcaagtaa tactaatgca ggattcttag gagcagtaat agcaggtttc
     3361 tttgctgggt atgtttgtaa gtttttaaag aaaaaaatta aaatatctgg gtcagcatca
     3421 ggaatgatgc cacttataat aatgccactt ataacagttg gattaactgg ttttttaatg
     3481 agtgttgtat tagcaggacc tttaggaaac ataaatactt cattaaatgc ttgggtagct
     3541 gaaatgtgtc aaaatggaac aaattcggtg gtattggctt taattttagg cgcaatgata
     3601 ggattcgatt taggtggacc agtaaataaa gcagcttgga tggcaggaaa tgcattattg
     3661 ttagagggaa tctatctacc agcgatattg gtaaactgcg caatagttat acctccacta
     3721 ggatatggtt tagctacatt cataagaaaa tcaaagtttt ccaatgcatt agctgaaaca
     3781 ggaaaaggca atatagttat gggtataatt ggaataacag aaggtgcaat tcctttcact
     3841 ttaacaaatc cattaaaatt agttcctgtt aatatgatag gatgcgcttt gggtgtagga
     3901 ttatcagctt tgttaggtgt acatgctata atgcctccag ttggtggatt atatggattt
     3961 atatcaatag gttctggatg ggcttactta gtaggtgcaa tatttggagc tttagtaata
     4021 gcaatattat caacaacttt agtagacttc aatgaagatg aaactacgga aaatacagaa
     4081 tttgatgaaa tagagttaga tataatgtaa attaaaaact taattttaga taattgagaa
     4141 ttgagtgtac tctcaattct caattaaatt ttgggaggat ttttatgaaa aagacaaaag
     4201 tacatgtagt tccacacagt cattgggata gagaatggta ttttacaaca tcaaggtcaa
     4261 aaatatattt aatgaaagat tttcaagata tattagacat attggaagaa aaagaagact
     4321 ttaaatactt tactatggat gcacaagctt ctctcttaga tgattattta aaatggagac
     4381 ctcaggatga atatagaata agagatttag ttcaaagaag aagattagtt attggacctt
     4441 ggtatactca aacagaccaa ttggttatat caggagaatc tataattaga aacctttact
     4501 atggaataga tagatgcaat gaatttggtg aacctatgag ggttggatat gtaccagatt
     4561 cttttgggca atcagctcaa atgccacaaa tatacagagg ttttggaata gatagttcat
     4621 tattttggcg tggagtttct gatgatatgg tagatacaac agagtttata tggaaaggtt
     4681 ctgatggttc aaaagtatta gcagttcaaa taccatttgg ttattactat ggaggtaata
     4741 taccagaaga tgattctgat ttaaaagaat atttaaaaga tataatcgga aaacttaagg
     4801 ttaaagcttc tactaataac gtatattttc caaatggatt tgaccaagca ccaataagaa
     4861 aaaaccttcc tgatatatta aaaaaagcta atgaaataga taatgaaaat gaatacgaaa
     4921 taagtgatat agaaaactat atagattcag taagaaatga aagaaatgaa tttacagagc
     4981 ttaaaggtga atttttaaat gctaaacata tgagaataca caaatccata ttctcctcaa
     5041 ggtcagacct taaaataatg aataacaaat tagaaaatta tattacaaat gtacttgaac
     5101 cattattaac attaagttat tcacttggaa atgaataccc tcacctagtt ataaaagata
     5161 tatggaaact tatgtttgaa aatgcagctc atgacagcat aggttcttgt aatagtgaca
     5221 caacaaatga ggatgtatac acaagataca aacaagccag agatttgagt ataaatttat
     5281 tagatttgca tatgagactt ataagtactc aaatcaaaaa taataatgaa gaaataacat
     5341 taacagtatt caatggattc ccagaagtca gaaatgaagt tgtagaattt gatacttata
     5401 ttccaggtca agattttgtg ataaagaata aaaatggaaa aattcttaat tatataataa
     5461 aagaaaaaga agatattaca aattatatat taactcaaac aataagatta aacccaagta
     5521 aaaaaatata tatacccaaa actgtatata gagcaaaaat tgcactttca gtaaaagata
     5581 taccaccaat gggatatact caactagttt ttgaattgga tactaaaaat aaatgtgaaa
     5641 tagaaacaag taattgcaag gaaatagaaa acaaatacta caaaataaca gccaataaaa
     5701 atggttcatt agaaatatta gataaagaat ctaataagac ttataaaaac caagctgtaa
     5761 ttgaagacaa tggagatgat ggagattcat acaactattc tcctccaaga aaagatattt
     5821 atgtttcatc tttagattct gtatcttcag tagaagtgct aaaatcaaac attcaagaag
     5881 aaatgatact taaatattca ttgaccatac caacttcact ggaagaaagg gctataggta
     5941 aagtttctgt gaaaatgcca gtaacaatga aaatcacctt agaacctaaa gaacaaataa
     6001 ttaagtttaa tgttaatata aaaaataacc aagcactatc tcatagagta agagttttat
     6061 ttaatacaga aatagcatct aagttttcta tagcagatca acaatttgga ataatacaaa
     6121 gaccaacagt attagaaaaa gaccttgcat tgtggaaaag agacaattac tcatggcaag
     6181 aaaaacctat aactatcgaa ccaatgcaaa gttttgttac tttagaggat ggacaaagag
     6241 gaatagcatt aataactggt tgtgtaagag aatatcaaat agttggtgat aatttagata
     6301 caatagcatt aacactattc aggtcatttg gatatatggg aagagaaaat ttactttata
     6361 gaccaggaag agcttctgga gaaaaaataa tagcaacacc agatgcacaa ttacttaaag
     6421 agttaaactt tgactttggt ctttacatat ataacaataa atttgatgaa gcaaatgtag
     6481 ctaatacagc taagagattc ttaacaccta tacaaatata tgaatatgct gattttttaa
     6541 atggtagact tatatttgca ttcaatgacg aaaaacaaat atatgaaaat gaatttagtt
     6601 tgatgaatgt aaataattca aatttcacag taagtgcaat aaagaaagaa gaaaaaggag
     6661 atggaattgt agttagaatc ttcaatggta tgaaaaatga ggatgctaag ggaagtctta
     6721 acatcaataa aaatgtcaat gatgcttata gtgcaatgct cgacgaaatc tatgataata
     6781 ctagcaagct taaagtaaat actaaaactg tagaggtaaa ttctttatca cattgtaaag
     6841 tacaaacaat agtaattaaa taatataaaa tagcttatac aagatttcaa aataaattgc
     6901 tagtactatg ttaatagtaa agccagatat tatgctattt ataagctctg taacactaaa
     6961 taacattttg taaaataaaa aggtgaacct tgtatattag gattcacctt ttttaaataa
     7021 atcaacattc tgttttaaaa taacatttat aagctgtttt gaaataatgc ttacaaatta
     7081 ttttaaaaca atacctataa atttttattt aaagaataca cataaatatt tccagattct
     7141 tttattctat agactttata attattaaag aagtctaatc taatttttgt taaaagtaat
     7201 tgtaactttg aataaatcac catccacact tatattcatc ttaccaccct gtaattctat
     7261 aaaacttttt gctatcgcta aaccaagacc agaaccttct gtgttacgtg atttatctcc
     7321 tcttacaaat ctctccatta tatcatctac actaaaatca atctcagatg cagacatatt
     7381 tttaagcata atatttactt tattatctgt ttctaaaata tcaatataca ctcttgaatt
     7441 tggcattgca tatttgctta tatttattat aagattttca aatactctaa atgtacgctg
     7501 actatctaat ggtaatatta ttttttcatt agaaaagtta ttctttacta ttagagatga
     7561 atctgcaagt ttgtcatcta attctaaaag tgtctgcttc attagagata caatatccac
     7621 atttactata ttcaaataaa catctccact atttgccttg cttacttcaa acaaatcttc
     7681 tattagatgt tgcaatcttt gtgactttct gtctaatgta tcaatataca acttacgatt
     7741 ttcatctgtt atactttcat cttttaataa atctatataa gtgattatag atgttagagg
     7801 tgtttttaag tcatgtgata catttgatat aagctctgtt ttcatctttt gactcttaac
     7861 ctcttcgtca acagcttttt taaatccagt ttgaatattt cctaattcat cttttatagg
     7921 attaaaaaat cctaaatctt tttccatatt tacatctaag tttccatctg aaattttctt
     7981 tgtaacatct aaaagttgat tatattcata tattgtcttt ttagagtaat ctgacagtat
     8041 cttaaataat actactgaat atattaatgc gagtattatt ccaaagaacc acatactaca
     8101 cattataact ataatcaata aatttattcc caaaattgta actatagttt tatttggctt
     8161 ttctttcaga tttattgttc taatttgatt aataactgct tttcctgctt tttttaacca
     8221 tacaaaaaac attcctgtca cagtatgttt tgtaaaatac tctcttattc caacatttaa
     8281 aatgtgtttt aaaagtatta taaatacaaa tattattgag aatattacaa accaataagt
     8341 taaattcatt aaacttacca atatactagc cattttagca cttatgtcat tgtttataag
     8401 gtactctaca taattcccac tcaaggtatt acttattata acagtaggat ataaatctac
     8461 aagcccctgt ataatagcaa gagcaataaa gagtatctct aacggtattc ttgataatgt
     8521 atcaaatcct agcaactctt tagattttct atatgggaat gctaaagcta caataacaac
     8581 tatagctaat ataattttta aaatcattcc agaaatctta taataagaat tattttctac
     8641 attgcttata tatgaaatta taccatcatc aaaataagta tttccataac catactctac
     8701 actatcacct ttacttatta ctttaggtac agcatatact aatgttatat tttttattgg
     8761 agataactta tatgaaattg aatttcctcc taaacttgta tccattaaat cttctcttgc
     8821 tttagataac aacatttgat taataatttt ttgatttact ccataagaat ccacaatatt
     8881 tacttttcca tctttatcaa aattcattac aacgtagaat ctatagtttt cttttatatc
     8941 tgtaatctgc ttttgtcttt cctcatcatt catttctgta ttttttgact gattttcatt
     9001 agaattttct tttgaagcat cattttttgt tgtatcaagt actcctaatt ctttattatc
     9061 attaaaaaat tcaaaattgc tttctttatc aactaaatag tactttaaat tctttcctga
     9121 cgctttaatg aaatgctccc aattttcaaa gttttcatta aaatctgcat taatactatc
     9181 atctgtataa taagtttctg aaccatcata agtatctttt tctttactta cattttttat
     9241 atagttcata gtttcttcat tgataaatag ctttgatggt tgtatcatct taccttcttg
     9301 ttcttccttc atattatgat ataaaacata acttccttta tatatactat ctacaaagct
     9361 ttcctgttca aatggattct ctgctccacc tttagcactt gcttgtatag tagaatattg
     9421 actaatcata cctaatgatg ctgataacat aagtataaaa actataatag taactatata
     9481 tttatcttta aattttttca attttgtatc caacacccca caccaccttt aaatatttag
     9541 gattttttgg gtcaatttct atcttttccc ttatattcct cacatgtacc ataactgtat
     9601 ctgtatttac agcattctca ttccaaactc tttcatatat ttcatcagca gaaaaaactc
     9661 ttcctgcatt tctcataagt aaacttagta ttttaaattc tataggagtt gtttttatat
     9721 actttccatc aatactaact tcttttgtat tttcatttaa ttctaatccg cctattgcaa
     9781 acacactatc gtcactttct accttttgct ctttatttcc aaccatgttt agatacttag
     9841 tgtaccttct aagttgtgaa ttaactctag ccaatagttc taatggctta aaaggttttg
     9901 ttacataatc gtctgctcct atattaagcc ccattatttt gtcaacttct tctgattttg
     9961 cagatagcat tattactgga aaattatagt tttctctaag cttcattgta agtgttatac
    10021 catccatttt aggcatcatt atatcaacta tagctaaatg tatttcttgg ttttctataa
    10081 ccttaagacc ttctaatcca ttgtatccct taaaaacatt atatccttga tttaatagat
    10141 atatttctat agcatctgct atctcctttt catcttctac caccaaaata ttatatgaat
    10201 tcattctaat ctcctttata ttaatttata aattttcttt acatatatga ttatacaata
    10261 tgatagaagt ttttccattt atatatatat ttatatcttt tgttctggtt atttgtattt
    10321 aatgtccatc ttagaaacct ccttctagtt t
//
LOCUS       DAEMGG010000135        10342 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEMGG010000135 DAEMGG010000000
VERSION     DAEMGG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.136"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(3..416)
                     /locus_tag="KPK24_003195"
     CDS             complement(3..416)
                     /locus_tag="KPK24_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300844.1"
                     /translation="MSKSELGVKANLVSELMMLSYFFIDLLLQKFIFKGEGIWFTFII
                     LLAFWVCNLMYNKKYNDLVDEASKLMLLKVDQIASKIMLYSMFVIAVLFSVNHTRALD
                     ISNLDIGIMLLAVLFIQSLSKLILYIYFDKKGICN"
     gene            complement(1250..1879)
                     /locus_tag="KPK24_003196"
     CDS             complement(1250..1879)
                     /locus_tag="KPK24_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG2300845.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(1958..2920)
                     /locus_tag="KPK24_003197"
     CDS             complement(1958..2920)
                     /locus_tag="KPK24_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG2300846.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQNQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(3682..7380)
                     /gene="cbpA"
                     /locus_tag="KPK24_003198"
     CDS             complement(3682..7380)
                     /gene="cbpA"
                     /locus_tag="KPK24_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBG2300847.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDTNAGNAEGDTNAGNAGGDANAGNAGGDANAGNAGGDANAGNAGGD
                     ANAGNAEEDSNAGNAEGDANAGNAEEDANAGNAEEDSNAGNAEEDMNALVVEEATAED
                     PTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAEL
                     IFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLT
                     IDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYY
                     SAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTIT
                     AKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFG
                     DGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLG
                     GAVAYGDLEKKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDDQG
                     VLYLWFSDQFKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQYEEFTIDDT
                     PPYLIIKRYDGLPFEEDVEKLFLDWILKDGGIPIITPKGEKLTDKKFMTISSQRLNEE
                     TMEPDTNLGSDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYKAEIIPADTE
                     TSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINRKEYKDPVE
                     LKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLK
                     LDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGN
                     GDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPN
                     LNKPDPDNGGNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGN
                     GGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNSKPDI
                     DTDGDGKPNINIDIDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDIDGDGKPDI
                     NIDIDGDGKPDINIDTDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQTGDQS
                     NIMLDLALMFISLFFLIKNLTNKYLRRK"
     gene            7649..8221
                     /locus_tag="KPK24_003199"
     CDS             7649..8221
                     /locus_tag="KPK24_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBG2300848.1"
                     /translation="MEKLVKILINIICIIALTFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNINYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNNNDEFKYSLNYIT"
     gene            complement(9221..>10342)
                     /locus_tag="KPK24_003200"
     CDS             complement(9221..>10342)
                     /locus_tag="KPK24_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG2300849.1"
                     /translation="IEDIESYIISNIDLKNLKIIIKKKKDICDYLNKISNYKKVKSTI
                     EKLETRGKMPKWYLELINKVKNILENMLYNRNLSDEQIFYLNLFLDIQNLFLGIYELK
                     SESVIFENTLDDNKIIAKMDTYIIIDYINASFEYLGYVLNGSALNSIDYGVPQNRERF
                     VLIGAKKDFLKNKKIETPKPIVGENYVTVRDAIGDLAEYEASKGSMDYCFEKNSNNIE
                     KDFYRKIILDSNKIYNHVCTDTRNIALKRFEYINQGNNFHSLPDELKGTYADPERTQN
                     TIYKRLVYDKPSDTVVNVRKSMWIHPIKNRAVSAREAARLQSFPDSYKFLGTKDSVYQ
                     QIGNAVPPLLGRVVAEKILNLFNDEPNEYLRDVFEALDG"
BASE COUNT         3384 a         1794 c         1113 g         4051 t
ORIGIN      
        1 acttagttac atattccctt tttatcaaaa tatatataca aaattaattt tgacaaagat
       61 tgtatgaata aaactgctaa gagcattata cctatatcca aatttgatat gtccaaagct
      121 cttgtatgat taactgaaaa gagaacagct attacaaaca tagagtataa cattattttt
      181 gatgcaatct gatcaacctt taacaacata agttttgatg cctcatcaac taaatcatta
      241 tattttttat tatacatgag attgcacacc caaaaagcta ataaaattat aaaagtaaac
      301 caaatacctt ctcctttaaa aataaacttt tgtaataaca agtctataaa aaaataggat
      361 agcatcatta actctgaaac aagatttgcc ttaacaccaa gctctgattt agacatataa
      421 aatcacccct taaaagttat atatttataa caccttatgt tataaatata taacttttcc
      481 aaataaattg caatagaata ttgaaaatat tcaataatat cttcataaat atattttata
      541 aaagatttta taataaacat tacaatacag tactaaatta gtttatttca tctatcatct
      601 caatcatatc atttactaac tattattcta atgagcatat aacattgaaa tttataatta
      661 tacaacctaa atgcctccca atgtaccctc aaaaacatca tttcctgtaa ctgaagtata
      721 attggtatat tttatcactg aaataaaaaa acactagtca ataaagacta gtattttcaa
      781 gtattttatc attctttttt taattattgg agacgaatca tcagaataat gcccataatt
      841 tatctatatg caaattctaa atttaaaata aagtttcaga tgaatttact attctatgct
      901 atgatacaac agaaaaaact gaaatctatg agtggtagct tcatataatc ctagttccta
      961 cagtaatcaa aactgaggtt tattctatat gtaactaatg ataaatttta ttataggagt
     1021 tattagctct atttgtgtta ctataagtag taattatata tatgataatt ttttaacact
     1081 tttctgttgg ctatatagaa agtggtttag aatttaaatt taaataaaat ttaaattcac
     1141 caaaaagaaa tctatattag aaaccactac tctattccac cagattgtaa ttttttgttc
     1201 tttatatcac aatgctatac catattaaat tgaaaaataa attttaaatt tatttgttac
     1261 aattcattaa ttcaaaaaaa tattttgagt catgagtttt gtaaagattt agccatgaat
     1321 ttaatgcttc tgattcaaaa acatctaaaa gtttcaatac ttgaaacgta aattccaacg
     1381 gagcgattcc tgaagccgta actaaatttt catcactaac tacagattcc gttttataat
     1441 atttttctcc agagtaattt ggtattacca ttttaagaaa ctctaagtca ttacttgtat
     1501 gatttctaac atctaatagc ccttttttag ctaggcctaa tgtagctcca catatggctg
     1561 caactataga atcttgtatt aaagcttctt taactttttc taatattatc tcatgattca
     1621 tggataacca agtatttccc cctggcaaaa ttaataaatc cttactttcc atattaaact
     1681 catcaactgt aatttcaggt aaaactttta acccacccat tgttataata ggatttttat
     1741 caattcctac agtaattact tttagtggct ttaatccttt tttaaaatat cttcctgtat
     1801 ttaattcagc agttaaatat gccacttccc aatctgacat agtatcaaat acatacatat
     1861 atacttttct atttttcatc ttttttactc cttatgtaat taatttatat atctaattat
     1921 aaaataactt aactgacata ttcagtcagt taagctatta aatttgataa taatcaatta
     1981 attctagtaa atcttcaatt attttcttcc taaggcttag tggttcaatt acttgtattg
     2041 tctttctata aggtaaaagt atatttggta tgtacttgca tattacttcc tcttcgagca
     2101 taaatattat tttattaggt gttctttctt tcaagtagtg ccctaaaaac caatgttggc
     2161 ttaaactatc taaagaatct atactccctt ctacaactaa ttgaattata ttttgttcat
     2221 cttttatttc aggtataatt ccttttataa aaaattcact tgctgaaaat tctgtcggtc
     2281 tgttaaatgt atttgatgtt tctatgattt cactaatcct atccactctg aaactacgta
     2341 cctcatttct catattacaa aatgcaacta cgtaccaatt attattccag tgaacaattc
     2401 catatggatt tataattcta tattttgatt ttgctttacg acctgcatga tagttgattt
     2461 ctacagatag ctcctttaat atactattct ccaatttttt aagtactgat tctatagaca
     2521 ctttcccaat tggttttatt acttcaaggg ctcttaagtg gtttgttaaa atatcttctt
     2581 ggttttgatt tgaatacatt tttaacttta atatcgattt atctaatgct tcgtctaaga
     2641 agtatccttc ttttttagca aatagagctg catgaagtag agatttttgc tcttctatat
     2701 caaaaaaaag aggtgcttta gtaaaattat ttaaaaggct atacccacca ttatgacctg
     2761 tatctgaaat tataggtact ccactcgcag aaagtacatc aatatatcgg tacactgttc
     2821 ttatatttat ttctaatttt tcagctatct gttttgctgt aatttttttg tcagagttta
     2881 acatccacag tatagacatc atattgtcaa ttttagccat gtagtttcac ctctttctat
     2941 ttgttatata aagttattat ataacttttt gattgatttt ttacaaaaaa acattagtaa
     3001 actgctaata atttatacta agatttattt caaatattta tagatagtct ttttctatag
     3061 ttaagttatc cattaaatta catagtagta tatccatttt agttgatata ttttcagcta
     3121 cctcccttga atattattac aataagtcta aatcttcttt cttccataaa tatattatga
     3181 ggaaagacta tcatctttaa tggtttaata gtataaaaga tattaattta aaaaaatcta
     3241 tttttatatc agatgggtat tgttttttat aaaactgaaa aaatcaccta atagaatcta
     3301 tgatttttta acataacctc cataaattag taccaattat cacttcaaat tcaaattttt
     3361 atctgaagaa tatacaatca tatttccata ctcaacataa tattgagtgt ttggcttgta
     3421 tttacaaatt attattctaa caaaaacaac tatacataga ttaaaatcac cttctaaaca
     3481 taattattaa aatttaatta atgatatcaa ttccattaat ctcttttaat aaaaaattct
     3541 cttttctata tgaaaattat ttcaaaactt ttaagttaaa tacaaaaatg actataaata
     3601 gatttttatt tacacaaatc tatttatagt cactatagtt tatccaataa aataataaaa
     3661 taaatatgta cacaataaat gttatttacg tcttaagtat ttatttgtta gatttttaat
     3721 taaaaaaaac aagctaatga acattaatgc taaatctagc attatattac tttgatctcc
     3781 tgtttgtaca ttttgaccta cattactatc taaactttca tctgtatcta tatttatatc
     3841 tggttttcca ttcccatctg tatctatatt tatatccggc tttccatccc catctgtatc
     3901 tatatttata tccggttttc catctccatc tatatctata tttatatctg gttttccatc
     3961 tccatctata tctatattta tatctggttt tccatctcca tctatatcta tatttatatc
     4021 tggttttcca tctccatctg tatctatatt tatatctggt tttccatctc catctatatc
     4081 tatatttata ttcggttttc catccccatc tgtatctata tctggtttgc tatttcctcc
     4141 attatctggg tctggtttac tatttccacc gttatctgga tccggtttgc tatttcctcc
     4201 attatctgga tctggtttac cattgccacc gttatctgga tctggtttgc tatttcctcc
     4261 gttatctgga tccggtttgc tatttcctcc attacctgaa tccggtttgc tatttcctcc
     4321 attacctgaa tctggtttgc tatttcctcc attacctgaa tccggtttgc tatttcctcc
     4381 attacctgaa tctggtttgc tatttcctcc attatctgga tccggcttgc tatttcctcc
     4441 attatctgga tctggcttat ttagatttgg attacctgta tcataacctc ctgagccagt
     4501 atctacattt ttatcaggct tccaataatc atctactcca tcattatctg tatctattgc
     4561 tggacgatta taatctgtat cagggtttcc atctccattg gtatctatat ttttatcagg
     4621 tttccaaata ccatctccat ttccaccttc tgtactaggc ttccattcac ctgtattatc
     4681 agtatctatg ttaatatctg gcttccaatc accactcgta tctatattga tatctggcct
     4741 tccatcacca tcagtgtcta tatttatatc cggtattcca tccccatcta catctacatt
     4801 tgtcatatct aactttaact tcttttcatc ctcactacct tcaatgtaat ttatatcatc
     4861 tccaatatat tttacagtaa tagtttgttc agtagtaaaa atatgatgat tgctatctaa
     4921 aggtctccat tcaatagatg cagtactata attatgactc tcactaccct tattatgagt
     4981 ttttaattct actggatctt tatattcctt tctattaata tagaattgta cgtaacctct
     5041 tggagaggca caatctggtc cttctttctt atctggtgaa atagtaacat ttaatgtaaa
     5101 cgtatctgtt ggtttaaaaa catcagtttt agctttagac tcttctacaa ctaatgaagt
     5161 ttctgtatca gcaggaataa tttctgcctt ataatatgct ctatgccccc aataagcttc
     5221 attaaaagga tatacatttg aaaactgtgt tgatgtaatg cttaattgat attttccagc
     5281 atcaacttgc tttgtaaggt catcacttcc taaatttgta tctggttcca ttgtttcctc
     5341 atttaaacgt tgagaactaa tagtcataaa ttttttatct gtgagttttt cacctttagg
     5401 agtaattatt ggaatacccc catcttttaa tatccaatct aaaaataatt tttctacatc
     5461 ttcttcaaat ggtaatccat catatctttt aataatcaaa tatggtggag tatcatcaat
     5521 agtaaattct tcatattgct ttaaaaattg agaacctgaa cctatatctt taacataaaa
     5581 atcatctggt tttatatttc ctccagtaat tttatctata acaactaaat taacagatac
     5641 ttcagatccc ttaaattgat cagaaaacca taagtaaaga accccttgat catctgtata
     5701 actaggtgct ccataaggat aaatttttcc atcaattagc attttaaaat catcaactaa
     5761 tgtagtacta actttatctt gtcctaaata ctttaaagat attttattca taaaaacttt
     5821 tgttttcttt tctaaatcac cgtatgcaac tgctccacct aaacttttga atgttgttgc
     5881 ttttaatgaa ccacctgtta ctataacatc tcctgttgta gcacttaggt caaaaatcca
     5941 atctcccact cctgtcatat aaggtaaaat cgttcctcca gttacaagta tacgatatcc
     6001 tctatttgtt cctggattaa cacatccatc cccaaatgca ctaccatgtg cacctccata
     6061 tgaataggaa agtccaccat taattgtaat atttccacta taaccagaac ctgtgctagg
     6121 atctactact gagggactta atgcattctt tgttgttgta tagtatcctc cacctattcc
     6181 agctccatgg tatgcacctt tagcagttat agtaccacca ttgattgtga tatctccacc
     6241 actaccatta ccgcctccac ctattccagc tccatatcct gtattacgac tcgattcttg
     6301 acctatagca gttatagcac caccattaat aataatattt cctccacttt cagtatttcc
     6361 accgccaata cctgcagaat aataatttcc aacaactttt aaacttcctc tattattact
     6421 atctagtagt gtaaggcgat ctcctttttt taacttgtct ccattttcta gagttacatc
     6481 tctaggaatt tttccaccct caggaactat aaactctcct tcaatatctc tatttacaac
     6541 agaatcatca atagttagag tagtaccttt tcctgctctc atacctggat aatatacatc
     6601 acctgactct agcttgtttt cactaccatc ttgtaatata acatgtgcat atgcaccacc
     6661 atctttatct ggtgttaaag taaatgggct attagatgca gttttaatat ttactccatc
     6721 aaatattaat tcagctttta catctttagg tataacaatc ctatgtgttg ttggagtatt
     6781 tggattatta ttttgaattg tcatttgttt tccactaaga atagttaaaa cgccacttgc
     6841 aaatttaaaa tcgacattta tttctccacc actaatttta aagtctccta cagatgtagg
     6901 atcttctgca gtggcttctt ccacaactaa tgcattcata tcttcttctg catttcctgc
     6961 attagaatct tcttctgcat ttcctgcatt agcatcttct tctgcatttc ctgcattagc
     7021 atctccttct gcatttcctg cattagaatc ttcttctgca tttcctgcat tagcatcccc
     7081 tcctgcattt cctgcattag catcccctcc tgcatttcct gcattagcat cccctcctgc
     7141 atttcctgca ttagcatccc ctcctgcatt tcctgcatta gtatctcctt ctgcatttcc
     7201 tgcattagta tctccttctg catttcctgc attagtatct ccttctgcat ttcctgcatt
     7261 agtactttct tctatagtat ctgcgaaagt atttgacgca ttaaatgatg ctactgatcc
     7321 aagtcccatt gagatactta aaatcattga tagtgctttc tttttacatt tttgttgcat
     7381 aaattccccc tccccttaat aatttttaat atacactgta tacatcctta tatacactct
     7441 aataattctt aatatacact gtatacatcc ttatatgcac tctaataatt tttaatatat
     7501 acttaaaact tatatttgtc aatgaatata ctgttttttt caaccttata tattataagt
     7561 attcattatt tgctaaataa ctacaataaa aattttaatt atataaaatt tatttaattt
     7621 tttatataat aatactaagg ggtaattaat tgaaaaacta gtaaaaatat taataaatat
     7681 tatttgcatt attgcgctaa ctttctctag cctttcgatt tacataaaat tatctgaata
     7741 taaaaaagct gatgaagtat atacggaact cagagaaaac actatcaata atagcaaaca
     7801 tcaagaactt tatgataaaa ataatgatta tagattctgg cttaaaataa acaacgtaaa
     7861 tataaactat cctgtagtac aaggatataa caatgatttc tacctaactc atgattttta
     7921 taaaaattat cttcctttag gtagtatttt catggattat agaaataatt ttgaaaatga
     7981 taagagttta atagtctatg gtcattatat gaaaaataaa actatgtttg gtcaactaga
     8041 aaactatact gatgaagtat ttttcaaaga aaataactta gtagaaataa attataaagt
     8101 acaaacttat acttatgaaa ttttttcagt atatactgct gacttaatta acagagatta
     8161 tttaagtata cactttaata ataatgatga gtttaaatat tctttgaatt atataaccta
     8221 aaaatcagta cacaaatctg atataaaggt agatatttca gataaaatta taactttgta
     8281 tacttgtaac tatgaattta aagatgctct tactatagtt catgctaaat taatttcaaa
     8341 aaagagacct ataagtaggt ttgtgtaaat gcagacttat ctacagtctt ttttgaacgt
     8401 aaaattggta agtatggaat aatatttata ttaaactttt gtaggcaaag aaatgaaaaa
     8461 tcaaagagaa ataattatta cagatttaga ttaccaagct gaattaagaa agtgtaaaac
     8521 tatggaggat gtagttggtg aagatagtat ataatagctt aacttataaa ttatcttggt
     8581 gatattaatg aagtttcaaa gtatatatta tttattgata taaataaaat taaatctgtt
     8641 tctaatagat gtacctttgt ttggagacaa actattgaag aaatataact aaaatgttga
     8701 agtagctacc tgactttatt ttgaaatgcg aacaagattt tggcattaag ataatttaga
     8761 aagaatcact ggtacacaag gatgtgaact tagaatgaat agaagtattc aggcataagg
     8821 agctttcacc caaataaaac atagtatgaa ctttaaaaga attctaatca aaggcaatca
     8881 aaatattctt tcagagttta tacctgtagt gttagcctat agtatttcta agtttcacaa
     8941 ataagataat cattaataaa tgtaggatgt atattcatcc tctcaaaaat attttttaat
     9001 cttacacttt gtaaattatt ctattttaaa attgtatttt tataccttta tttcttatga
     9061 ccgttttcaa gctcaaattt tgcataaata taaattaatg aaaagcaagt aaaaaaagaa
     9121 ctgtcgcaca gcaatttaaa attactcatt cgacagtcct tatttcgtgg attaatgttg
     9181 gataagaatc acaagaataa tacccactat tttatttgaa ctatccatct agtgcttcaa
     9241 acacatctct aagatactca tttggttcat cattaaataa atttaatatc ttttctgcta
     9301 ctactcgacc taataaaggt ggtactgcat ttcctatctg ttgatataca gaatcttttg
     9361 tacctaaaaa cttataacta tcagggaatg actgcaatct agccgcctct cttgcgctaa
     9421 cagctctgtt tttaatagga tgtatccaca tagattttct tacatttaca acagtatctg
     9481 atggcttatc atatactagt cttttataaa tagtattttg agttctttct ggatcagcat
     9541 aagttccttt taactcatct ggcaaagaat gaaaattatt tccttgattt atatattcaa
     9601 atctttttag tgcgatattt cttgtatctg tacatacatg attgtatatt ttattgctat
     9661 ccaatattat ctttctataa aaatcttttt caatattatt actatttttt tcaaaacaat
     9721 aatccatact acctttagaa gcctcatatt cagctaaatc gcctattgca tctcttactg
     9781 taacataatt ttctccaact ataggcttag gagtttctat ctttttattt tttaagaagt
     9841 ctttttttgc tcctatcaat acaaatcttt ctctattttg aggtactcca taatcaattg
     9901 aatttagtgc acttccattt aatacatatc ctagatactc aaaggatgca tttatataat
     9961 ctattataat gtaagtatcc attttagcta taattttatt atcatcaagt gtattttcga
    10021 aaattacaga ttcagatttt aactcatata ttcctaaaaa taaattttga atatccaaga
    10081 ataagttcaa ataaaatatt tgttcatctg ataaatttct attatataac atattttcta
    10141 atatattttt tactttattt attaattcta aataccattt tggcattttt ccccttgttt
    10201 ccaatttttc tatggtagat tttactttct tataattaga tattttattt aaataatcac
    10261 atatatcctt ttttttctta ataattattt tcaaattttt taagtctata tttgatataa
    10321 tatatgattc aatatcttct at
//
LOCUS       DAEMGG010000136        10293 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEMGG010000136 DAEMGG010000000
VERSION     DAEMGG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.137"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            205..984
                     /locus_tag="KPK24_003201"
     CDS             205..984
                     /locus_tag="KPK24_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG2300850.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1294..2115)
                     /locus_tag="KPK24_003202"
     CDS             complement(1294..2115)
                     /locus_tag="KPK24_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBG2300851.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGNVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEIWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(2790..3608)
                     /locus_tag="KPK24_003203"
     CDS             complement(2790..3608)
                     /locus_tag="KPK24_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2300852.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQVFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            3776..4357
                     /locus_tag="KPK24_003204"
     CDS             3776..4357
                     /locus_tag="KPK24_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2300853.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            4354..4548
                     /locus_tag="KPK24_003205"
     CDS             4354..4548
                     /locus_tag="KPK24_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300854.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(4712..6454)
                     /locus_tag="KPK24_003206"
     CDS             complement(4712..6454)
                     /locus_tag="KPK24_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG2300855.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            6883..8259
                     /locus_tag="KPK24_003207"
     CDS             6883..8259
                     /locus_tag="KPK24_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG2300856.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            8546..9217
                     /locus_tag="KPK24_003208"
     CDS             8546..9217
                     /locus_tag="KPK24_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG2300857.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            9406..10203
                     /locus_tag="KPK24_003209"
     CDS             9406..10203
                     /locus_tag="KPK24_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG2300858.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDVNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         3803 a         1247 c         1568 g         3675 t
ORIGIN      
        1 tataattaac actacttttt aaatataatc aataccgatt ctataatatt aacactattt
       61 tttatgctat ataacaatta ttgaaaatga tataaatata atatctcaaa taatatgaat
      121 ataacatctg aatagagggt gttttatttg taaaaaataa gtttacagtt tgagcgtata
      181 aacttatggt aaaggagaat aattatgagt tataagaaga ttgcaattat aacgggtgct
      241 aatagtggtt ttggtaaaga gtttctaaag ttattaataa atgaagaaga aataacagaa
      301 atatgggcag ttgcaagaaa caaggcgaga ttaaatcaat tagtagatga atttggaagt
      361 aaggtaaaaa ttttttcaaa agatttatca aaaatagaag aagtaaaaga aattggtaca
      421 tttttaagta aagaaaatgt atgtataaaa taccttataa acaatgctgg ttttgcaaaa
      481 ttctgttcat acaatgattt aagtatagat gaatctatta atatgattga tttaaatatt
      541 agtgctgttg tagcattggg acttatatgt attccatata tgcaaaaggg aagccatatc
      601 atgaatattt cttcacaagc atcattccag ccattgccat atcaaaatat ttatagttca
      661 actaaagcgt ttataagaaa ttatacaaga gcattaaatg ttgaacttaa gagtaaaggg
      721 ataaatgcaa ttgctgtatg tccaggttgg atgagtacaa atctatttaa gagaggtata
      781 gttgtagctg aaaaagggac aaaaaatttc ccaggcatag tgtctccaga cattgttgcc
      841 aaaaaagcat tgaaggatgc aaagaaaaat aaggatattt ctgtgtatgg tataaataca
      901 aaatccagtc atttattagc aaaactattg ccacaaaaaa tgatgatgaa ggtttggtta
      961 atgcaacaac atattaagga ataagagttt cggtaattaa gtatcagttg gagattcaat
     1021 ctgttatata ttatgagaag ttttgttgag caaataataa ttgtgacaaa acggcttatc
     1081 tagtgccgat aagccgtcta taatgtatat aaaatatatt tttgatttta ctttatttag
     1141 atattgaagt attaaaatat gtaggacttg aaattttata aagataaata ctatgatatt
     1201 ttgtaaaaac tgagatatgt tgatattaca tagtttaact atactgttaa attgaagttt
     1261 tcttattttg cattatgctt attacttaat gatttagact ataatttcca taccatcata
     1321 tgcaaaataa atgttatcaa gtttcttttc taattcactg taataaccat atgattttcc
     1381 ccatatttcg tcaatatgtg taatgataat ctttttaatt ctataatagt ttttaatttc
     1441 aacaatctca tctaaagtaa atatttcatc tttaaatggt gtgtctttaa gttttgtacc
     1501 atcatttaat ataccatcat cagaaactag acttattatt aaaacatcag catcaaagta
     1561 catatcacta tgagtaaatg gcttgggatt acagcaggca tatatgactt ttttttcatt
     1621 ctctttgata acatagaagg ttatattcct gtctgggtta ttgttaatca aatcaatatc
     1681 aaaattattt atctttacat gcgaagtttc attaatagaa atgcaattta taacattacc
     1741 ataatattta agacaatcga gattcgttcg gtttatatct tcaattactt caggaagcgc
     1801 ataaaagcca attggattat tttctcctgt gataaagtca catcctattt tttcgacaat
     1861 tctaataccc ttaacatggt ctgggtcatg gtgagaaatt gaaatatgtt ttacttccga
     1921 tattttttgt ctgttacaag ctactgcaat atcttcaggt gtgtctatca gcatttttat
     1981 atcatcaata tataaacttg gccctaatcg ttcatatctt ccgccttttt gtctggcttc
     2041 ttcacatatt ttgcatttgc aaaatgaact tggaataatt gacataccgc cactaccaac
     2101 aatttttaat ttcaaagtta ttacaccacc ttcttaaaat attagattat ataaataatc
     2161 tacaatcaca actgattaac aaaattatga gttaataaat tatatcacat gtaaatatga
     2221 aagaatagta tgaaattttt ataaacatat attaaatgtt tctcaatgaa attgactttg
     2281 tatatgaact gaatgactta ttcaatatgg ataagacatt tttcttgtaa taagatatga
     2341 ttgtcctata aggtttatgt aattttgata taatttgata taatatattg attaacaaca
     2401 tacatgttat tggtgagcat atttctatag aatggaatat ataatttttt atatttatca
     2461 ttgtattatt gtctaaagaa agttaaaaag agtagagcac aaaggaatta tttgtacttt
     2521 actcttttat tgttttgtta aaaaaactaa tttatattag tgattaaaat atatttaact
     2581 agagtataag gatagtatat aatcttataa ttgatttatc tgacaaatta agataatatc
     2641 ctaattaaat tgtagatatt tatacataac actaaaagta atttgtttaa caaattaaga
     2701 taatatccta atcaaattgt aggtatttat acataacact aaaagtaatt tgtctaacaa
     2761 attaaaatat tatccaatta atataaaaat tacttctttt taactgggaa gcatacctct
     2821 gttacccaat tatcaggatt actatcgttg ccagggctta catgataaat attaaacata
     2881 ggaccatcca catcatagtt attatctgat atccaattac caatagcctc acaagcgtct
     2941 gcaacttgat tgaaatttcc attaacaatt gcagttgcag cagtcacact tggaacagtc
     3001 ttaaatttaa catgttcagt atctttatac tttccagata cagcaacttg tacttcaaca
     3061 tctacataat cttctttata tccagtatca taaaaaactg ctttagaata ctttgggggt
     3121 tcaatttgaa cattctggtc ttttgtctct aaaaatactt gatgccataa cattccttcg
     3181 tcttggtaag ttggtataac ccttctaagt gtcatcatat attttggtgc aaaattcttt
     3241 atagttacat cataattttt cataatatca tttcctccaa ttcttttaat agtggtttct
     3301 atttttaata gttttttctg tgtatcttct aattgttctt ttacttgaga atggtggata
     3361 tttagatatt ttatgagact ttctttatcg ttatattctg tcaaaatttc ttttatagaa
     3421 tacaagccaa agcccatatc ttttagtgca tggattctgt ttgtagtaga aagttggtct
     3481 gcactatagt atctatatcc attagtttta ttcgtatgag aaggagttaa aagtcctatt
     3541 tcatcataat gacgtaacat acgaatacta attttactta gctttgaaaa atcgcctatc
     3601 ttaaacatgt ttttgtctcc tttataaagg tacttttagc ttaatattaa tataaaccat
     3661 atcataatgt tagagtcaac taaaattata attaaattat attgacatat gatgaaaaaa
     3721 atattatact gaacatgttc agaaccgaaa atgttaattg ttttggaggt ctattatgaa
     3781 aagtaatgat aaagatgcta gagaacgtat aattgaagtt acattgaatc ttttaaatga
     3841 agtagatgat attgaggaga ttacagttag aaaaattgct gagcgtgcaa atgttggagt
     3901 tggtttaata aattatcatt ttaaaacaaa ggataatttg ttgagtactg ccataggaga
     3961 tgtgatgtca aatataatag cagagctata tgacgatagt gtatatactc taagacctat
     4021 tgaagattta aaaaatttat taaaaaaatt gtgtgatact ggattacatt atgaaaaagt
     4081 gcttcctttt gtattgaatc aatgtataac caatggagat atgcaagcag aacttgatat
     4141 tgtccctatg cttcgtaaaa tttttggaaa taaaaaagat gaaatgagtc taagaatcat
     4201 tgccctgcaa attattttac caatacagat ttctgcttta tctacagaat catttcagtt
     4261 atacagtggt ataaatataa agaataaata tgagcgagat aaattcattg atattttaat
     4321 agaaaatatt attggagagg atgttgatgt aagatgaaaa ttataactat tggttcaagt
     4381 cttatcactg ttttactttt tctttcaact atggtttgtg ggttctggat taagaataat
     4441 aaggtaacag atgcaagttc tattaagttc catatgaata gtgctatttt tacagggata
     4501 tttttattga tttccacaat ccttttaatt atatatataa aaaaataata atattattaa
     4561 taaataaagt atcagaaatt ataaagacaa gagtcttaaa aatttctgat actttaccat
     4621 ataattttat taaacattaa ttgtgtgtga aaaaatatca cttatttcaa tctaatacta
     4681 aatcttaaga gtgagtataa agtattagaa attaagaagc attttttagt tcaagtggat
     4741 tatctatacc aaaataatca tataaagtag agcttatttc ctctaaattt ccattttgga
     4801 aaggtaattg taaatttacc ttttctacca catctttaaa tccagcatca gtattttgat
     4861 ttaaaaaatc catatataca tcaacagctt ttctgtaatc ttcttgtgat aattgccata
     4921 tctgaagtga tacatcagat gcaagaccat aactcaagta ataaaaagga acttggaagt
     4981 tatgagatac aagtatccaa aaaggagcat ttttttctct aagtatatta ttggaaactc
     5041 catattcttc ctctaaatca aaaaacaatt tattgatatc atttagactc atatatggat
     5101 tcttgtatat aatttcttga aattcatcat aaagacaagc atcaataata gtatttaaaa
     5161 caatcgataa gtgttctttt tttatagctt cagattgttt accaaaaaaa tcatcaaaat
     5221 atttataaaa tagaatttct aaagatgttg aatgaacttc acatacatct atacttggtt
     5281 gcatcttatt atttaagtta atactgttgt agtaattata aaaatgacca aactcatgag
     5341 caaaactagt aacacctaaa aaagaattgt cccatgtatt aaacaagaaa ggtgctttat
     5401 atttatttat ataagtagta tatcctccag gtgatttgtt ttttccagaa gaataatcgt
     5461 acaagtcata ttttttcata tagtcgaagc tctcttttag ttttggagat atatcttcaa
     5521 gtactttatc aaacttccta aatgagcgat ttttatatac tttaatataa atagaagaat
     5581 cagatggttt agatgaaatc tctctatata atggaactat gtaatctttt acatcttgtc
     5641 taaatttttt agcttcttct tgagaatagt ctttgtttag gttcatatag gcataatcta
     5701 tataattctt aaatccttgc ttacttgcta tttcagttct tgtctgtata agttctaaga
     5761 atatgttacc aaatatagga ttgtattttt ttaagtaatc tgaataaatt ttcacatatt
     5821 cttcagggct aagatttggt cttgacaagg cttcttcaaa gtctacctct ataccatcta
     5881 ttgatactgt agatttagct aataaatctt catattttac aactaggtct tgctcttttt
     5941 taagcaaacc ttcaacttct ttagaattaa gttttccaat gttctttaga taattaaatg
     6001 tatcctttcc aagttctttt tctaattctt tagagaattt agaagttaca aatctatctt
     6061 gaaagtcttt gtatgacatg tcaacatcta ctgatttact cataagatac ttatattcct
     6121 tagagtaaaa ttcgtctgat gaatctatgt tatttctaat ttttgctata gtaagtgtac
     6181 tgtaaaaatc ctgataaatt ttatcaactt cattaaataa atttaattgt tccttagctg
     6241 ttttagctgt taaaagtttg tcgttacact tgttaatgtt ttcgcaaatt gactgtatat
     6301 taggacgctt atattccatt ttttcaaaag gaatagttcc tctaacagaa tctttttttg
     6361 tatcattagt ataatttctt atgtatgatg caaatataat aaaaactgca attaaaatta
     6421 gaaaaacaaa tattttcttc ttggatttaa ccataaaaat cctccaatca tggatatagt
     6481 tattaattat tttactatat taaatataaa aaatgcatgt taagaaaatt taacataaag
     6541 atatagataa gattataaat aatataaaaa atttaacttc aaaaaagtat atgaaattta
     6601 tactggtaaa atgataaata aattttaagt acaaaatagt aaaagctatg atagttttag
     6661 acctattata tgtaaaaaat atagatgttt ttggtgcaat ttaaattaaa agatatatat
     6721 aaaaatttaa aatattccaa acagaaatta taaaaattac tatcattgtc tttatacaat
     6781 atttatacta taatagaata ataatttaga ggaaagctct aaaaataaac aaattaaggg
     6841 tcttaacagt agtgatttta tgttaaggag gtataaagaa agttgaaaac aatagttaga
     6901 agacttattc tctacgcaga tggaatgcgt cctactcaaa taatggtatc tgggtttgct
     6961 gcgattattg taatcggagc attgttgtta acactcccga tagcgtcgca gagtggagag
     7021 agtataggct tattaaatgc tctatttacg gctacttcag cggtttgtgt tactggtcta
     7081 gttatggtag atacagcaac ttattggagt ttatttggac agatagttat aataacattg
     7141 atacaaattg gtggtcttgg atttatgacc gtagccacaa tgttttcact tatggcacga
     7201 aaaaaaatac aactaagaga aagattatta atacaagagt cattaaatca agctgattta
     7261 tcgggacttg taagacttac aagatttgta ttaataataa ccataacaat agagggaata
     7321 ggtgcactgg ttttatcaac tgtctttatt ccacaatttg gtttgtctaa agggatatgg
     7381 tatagtgtat ttcacgcaat atcagcgttt tgtaatgccg gatttgactt gatgggcagt
     7441 gtaagtggcc catttacatc gttaaattct tatgtaaaca actttacagt atcaatgact
     7501 gtttgcgcac ttatagtgct tggagggcta ggattccctg ttgtacttga tattgtaaga
     7561 aaaagaagat tttcaaaatt aaatgtgcat tctaaggttg tattgttttc aactgctaca
     7621 cttatttttg tgggtgcatt atttattttt cttatagaat ttaatcaaaa agctactatg
     7681 gcggacttgc ctttaaaggg aaaagtacta tcagcaatat ttcaatcagt aacagcaaga
     7741 actgctggtt ttaatacgct tgatttagct actttacgag aaagtagtgt atttgtaatg
     7801 ataattttga tgtttattgg agcatcacct gcatcaactg gtgggggtat aaaaacaaca
     7861 acactagctg ttttaatcat tacagttaga agttttctat caggaaaatc agatatagaa
     7921 gcttttgaaa gaagattagc tccatcaact ataaaaaaat ctcttggtat atttgtaatc
     7981 agtatatcag cagttatttt tggaacattg attatttcaa taactcagcc taattttaca
     8041 ttagttcaat cagcttttga ggttacatca gcacttgcta cagttggttc aagtttagct
     8101 ggaactccaa acttaaatgc tttaggtaag attataataa taatctttat gtttatgggt
     8161 agagttggtt ctcttacttt atttatggct atactttctg gtggtagaag aaagagtcaa
     8221 ccaatcagat atgcagaagg taaaattatg gttggttaag ttaccaagga gtaatataaa
     8281 tttataatgg atttagagta gcactgtaaa tctgttgata aaataaatga gcctagatgt
     8341 aattatatga agaaataaaa tcgactaacg aacaaaatta attaatgaaa taaaaaaaca
     8401 gggaaataaa atacaaataa aaaggtaaat caaaattaaa ttaatctgtg tatataaaat
     8461 tatatattgt gtataagttt tgtatgtgta aacaattaat ttaagagata ttatataaat
     8521 tgtgtattag ggaggaagtt tcgagatgaa gcaatatata gtcattgggt gtgggagatt
     8581 tggaagttca gttgcgtcta ctatgcatct tttaggacat caagtaatgg caatagacaa
     8641 aaatgaagat tcagttcaaa gtatatctga caaggtaacc cattcactta tagtggatgt
     8701 tactgatgag caagcgttaa ggtcattagg tttaggtaac tttgatgtag cagtagttgc
     8761 aataggttct gatataaggg catctataat ggcgactctt atagccaaag aaatgggtgt
     8821 agagttgata atatgtaagg caaaggatga attacaagct aaagtgcttt ataaaattgg
     8881 cgcagataga gttgtatttc cagaaagaga tatgggagta agagttgcac acaatttagt
     8941 ttcagataat atattagacc atattgaact tgacccagag tattcaattg ttgaaatcgt
     9001 aactccaaat agttgggttg gcaagacact tatagagctt gaattaagag ctagatatga
     9061 gataactgta cttgctataa aaacaggtaa aaatataaat gttacacctt ctccagatga
     9121 ggaacttaca gccggaagta tcctagttat aatcggtcaa aatactagta taacagcgat
     9181 aacatctgga aataagggga taattagaag aagataattt actatttaat atatatttaa
     9241 ttgcaatgaa agtagatagt atcatataat tatgaatagt tatatgatac tattttttta
     9301 atcgaaggta gtatttattt gtaagattag ataaagagaa attaaattaa aagtaaggag
     9361 gctgtgctaa tataaaaatt tataagttat tagcatttag ataaaatgat tacaaatata
     9421 aacagtaagg ataatgaaaa gttaaagtat acaagagcac tattaaaatc aaagaatagg
     9481 aataaagagt caaagttcat aatagaggga tacagaatag taatgcttgc acttgaatgt
     9541 atggcaaacc ttgattatgt atttatcaat gaagaatttg aaaataagaa agaacatgta
     9601 aaactattag aagatttgga taaaaaaaac acaaagatat acaagactac taataaaaac
     9661 tttaaagaat tagtggatac agaaaatact caaggaataa taggtgtagt ttcatttaag
     9721 aaaaaaaaat taagtgaaag tataaataaa aaagataaat ttgtattgat tttagacaga
     9781 atacaagacc caggaaatat ggggactata ataaggactg ctgattctgc tggagtagat
     9841 gccataatag cactaaaggg atgtgtcgat atatacaacc caaaagtaat taggtctact
     9901 atgggttcta tttttgatgt gaatataatt gatgcttcac aagatgaaac tgtggacatg
     9961 cttaaatcat tggattttaa tatagtttca agttacttaa atacagaaaa tttttatgac
    10021 aaaatagatt atggctcaaa agtagcattg gtgataggaa acgaagcaaa tggaataaat
    10081 gaagaacttg tatcaaagtc tgatattttg gttaagatac ctatatatgg taaagccgag
    10141 tcgttaaatg ctgcgataag ctctgctata ctgatgtatg aaataaaaaa atacttaatt
    10201 taatgtattg taataaatat aatgtcatgt tataatcttg aattaaatag tagagtatca
    10261 aaaatagtca atatatatta aaaaaataat taa
//
LOCUS       DAEMGG010000137         9927 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEMGG010000137 DAEMGG010000000
VERSION     DAEMGG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.138"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(548..1528)
                     /locus_tag="KPK24_003210"
     CDS             complement(548..1528)
                     /locus_tag="KPK24_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300859.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(1692..2564)
                     /locus_tag="KPK24_003211"
     CDS             complement(1692..2564)
                     /locus_tag="KPK24_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2300860.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITFKYEK"
     gene            complement(2728..3279)
                     /locus_tag="KPK24_003212"
     CDS             complement(2728..3279)
                     /locus_tag="KPK24_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300861.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(3299..4201)
                     /locus_tag="KPK24_003213"
     CDS             complement(3299..4201)
                     /locus_tag="KPK24_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG2300862.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDD
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(4216..6588)
                     /locus_tag="KPK24_003214"
     CDS             complement(4216..6588)
                     /locus_tag="KPK24_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG2300863.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(6809..7498)
                     /locus_tag="KPK24_003215"
     CDS             complement(6809..7498)
                     /locus_tag="KPK24_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300864.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVYDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(7739..9118)
                     /locus_tag="KPK24_003216"
     CDS             complement(7739..9118)
                     /locus_tag="KPK24_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2300865.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENVNKYIRIKGTMTRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            complement(9112..9825)
                     /gene="rgbR"
                     /locus_tag="KPK24_003217"
     CDS             complement(9112..9825)
                     /gene="rgbR"
                     /locus_tag="KPK24_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG2300866.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT         3238 a         1715 c          917 g         4057 t
ORIGIN      
        1 tactatgttc accacactct gggcacttgt gattatttac attcataaat actcctcctt
       61 attagtatag ttacatgtca aaaacttaat taatgtattc tatattactt attatatatt
      121 ctataataaa tcatattcta atataataaa acaaattaaa taaaaaaaaa accataaatt
      181 ttaaatttat ggattaattc ttgtaatgat aactctattt gaggatgtga gggagggtta
      241 tcattacaat agggtattat attatggttt ttaagctagt ctttctacac ctcatatgat
      301 acatatgcta tatgaatttt atatgatttt tacataaata ttttatgaaa tttaaatttt
      361 ttagtcattt tgaaatttgt aaaaagttct ctaaaaatcc acagcatcat aaacagccaa
      421 aatttaagta aaacatcata aaaaaatttg tccaataatt ttaacaagta atctcacctc
      481 attataatta ttgacacaaa cttaaataaa caatctataa atcttctata aatcttaaac
      541 tttcccccta ttgatacaca atttctagtt tgtcaatttt attttgcatt tctaagaaaa
      601 tttcttcaac agaattgtta aatatattgc ctaaatagat tcttttatct ttttgaaatg
      661 aataagattt tacactacca tcagaagaaa tatataaatg actccactct gctccatatt
      721 ttgaaaacct ctcctttgta tctttaaacc tcatatcttc tatttttatt gctttttctc
      781 tgtctcctct tactacagtg tgaccccttt ttattaaact ggttattttt atatcttctt
      841 ttaatttaat acgtgtatca tcattttttg aatatacttt ccatatcact ttatgacgat
      901 aattatctaa ttcctttatt aagcttctat caatagtaaa actatcttta gttaccacaa
      961 ttaaatcaaa atggtctaac atatattgaa atatttctct catactttta ttttttaaaa
     1021 tatcctcacc aataacctct ataaatctac agcctttctc atacatgcag ttcaataaac
     1081 ttgtgatttc agaaaaataa tcatttgtat tattatctcc atctatttct atagtaaagt
     1141 acaaaggagt aatgtatgat ttatttcctg ttatctcaaa tacacttgat agtgtctgta
     1201 tatcatcttc aaacgttaag tacacattct cataaaacat aaaaaattcc ataacttttt
     1261 ctttcatatt atggatattt ctatatctat tggataatat atttattata tcgtcagtag
     1321 tatttttccc atcacattca gccagtataa caatttgctt ccaacttaag ctatccacat
     1381 ttccgatact ttttttatat ccatcttctt cgtcataaat aggaccaatt ataatgtttt
     1441 cttttggcca cagatgaacc ctagttccat cttttaattt cggatattta ttttttacag
     1501 actcataaaa tctacttgta tcacccatga ttctttacta ccccccatag tttaaatatt
     1561 ctatgctaaa aatagcactt tatttttatt aattcaacaa ttattcaaaa ttggtcatta
     1621 tatatgttta aatggattta acatcaacct tatttatatg ttattgatgt ttatatatgt
     1681 tatataaaat cttatttctc atacttaaat gtaatcttaa ctatctctgg tttacaatta
     1741 agcctaatta aaacttttga gcctcctaag cctttattta aaatcatagt gctattatct
     1801 ttttcatatt ttccccagtc atacttagga aagaactctc tatttggaga taatacacct
     1861 ccaacaaaag gtattcttat tatacctcca tgaacatgtc ctgctaaaac caaatctgct
     1921 ccccattttg catatttttc aaagaaaaat ggtgtatgtg ctaaaagaat gttatattca
     1981 ttttcattaa ctttacctag attgtcttct acaaaattgt cacttagttc taattttttg
     2041 ttattagcaa ataaatatgg atagcattcc aatggtataa ttaaaccata taaattaaga
     2101 tggctattac ctctatatat tcttacagat tcattttcca tatttattat tttctttgaa
     2161 tatagttcct taaaataatc cttatataga tgtttatact tttttattaa agatttttgt
     2221 tcatgatttc caattatatg atatacttca tatttttcaa ccaaattatc tattaaatct
     2281 aatgcaactt gaaaattttt attttctcca tctactaaat ctcctgttat aaatataaca
     2341 tctgggttta atgattcaat tttatctatc aataccttat tatttttatc aaacacctta
     2401 ttatgtaaat cactcacttg aactatttta aagttatcaa attcctttgg aatttttttg
     2461 ttgaaaatag tatattctgt cgtttctata tgctgagttt catacttata cagaatatac
     2521 aataatatta atactaatac tgtcattgaa acctttataa tcatattctc ttcctcttta
     2581 ttctattttg ttaatattat tataatgctt ttatgttttt tttaaaacaa taacatcatc
     2641 acatacatat aaatcttttc gacattataa aaataagtta tccttttcta aaattttcat
     2701 ttattttaga aaaggataac tataaaatta tctataaacc tctatattgt gaaatatagt
     2761 ttttccttta tttaaatttt tatatatatg ctctaaagtt ccatcaaacc taatagaatg
     2821 tccagactct aaaatataag ttttcccatc cacattaagg tcaagtttac cttctattac
     2881 gagcacatat tcttctaaaa catgactatg tgtagatgaa acatgagtac agttttcctc
     2941 aagctcaatt ataaaaagtt caaaccttct ttgagggcta aaagggtata ttggatataa
     3001 cttcatgttc cctttttgct caacaatagg ctctacttct tctttcttta taacctcata
     3061 ggtatttgta ttttcattta atagtgtagt aaaatttatt ttaaatccag atgaaatttt
     3121 ccataaagta gatactgtcg gacttgattc acctctctca atctgtccaa gcattgcttt
     3181 actgacacct gtcatctttg aagctttgtc taaacttatg cccatttctt gtctcaataa
     3241 ccttaaattt tctcccacat taggaaattt attatccatg ttaaaatcct cctgatagct
     3301 atttgtattc tatgattttt actcctgtaa gttctactat ctcttttaat tttaacatat
     3361 gattgttaca aatatcatca aattcatata tataatcttc aagccctaat gccttatatt
     3421 tatcgattcc aaatttatga tatggaagta actccatctt gcctcctgga acattttgtt
     3481 tgacaaataa tgcagtattt cttatatttt cttcactatc atttactcca taaataattg
     3541 gcactcttat aaccatagat ttattcaact ttgaaagcct acatatatta tctaatataa
     3601 ttttattact tactcctgta tattctttat gaatattatc atccatagat tttatatcta
     3661 caaatatgtg gtctattttc tcaaatacat catttacctt attccaatca aaatatccac
     3721 aagtttcaat tgcaatattt atgcctttat cataaaaaat atcaactaat tctcttaaaa
     3781 aatcaatctg aagtgtaggt tcacctccag aaaaagttat ccctccatta gattcaaaga
     3841 aaaatataaa atctttttca acttcctcta ctatttcttc aacactcatt tcttcggtca
     3901 ttatacaaat ggcatcttta agacatacct tgatacactc tccacataaa tcacatttat
     3961 tatttatttg cgctctatca aatagtgaac ttatattttg aggacaaaca tctatacatc
     4021 tattacagga tacacattta tctttcataa cacctaattt tactatgtta ctccaggaat
     4081 ctggattaga acaccactta cattttaatt tacatccttc taaaaatata gtagtcctta
     4141 ttccatctcc atcatttact gaaaaatgtt gtacttttac aactctacct tttttactca
     4201 taatctacct cataattaaa aattgctatg tgaatttcta gctattatag catcttgcat
     4261 ttctcttgat aaattaacaa attgagtact atatccagca accctcacta ataaatcttt
     4321 atattcttct ggattttctt gagcttttaa tagagtatca tttgatatcg tattaaattg
     4381 tacatggaat cctcctaaag caaaatatga tttaatcata gatgttagat ttctaagtcc
     4441 tctttcagtt tccaccaaat cttgattcag tcttaaattt agaagtgttc caccagtatg
     4501 aacatcatga tttatttttg atgcagattt cattgctgct agtggacttg ttgtatcagt
     4561 accaacaaat ggagaaactc cctctgtcaa aggttttgtc gcttttcttc cacaaggagt
     4621 tgcacctact atttttccag ttggtacata gtttgatata cccataaatg cactattaaa
     4681 tttagagcca aaaatatcct tgtattttct agtttcagta taataaaaat cagaaacttc
     4741 aattgctaat gaatctacat aatcattatc atttccatac ttaggaacat ccaacgcaag
     4801 ttttcttaat tcttcataac cttcccaatc attatttagt gcttttgtta attcttctaa
     4861 cgtagtcacc ttatcctcaa atactaattt ttttatagct gccaatgaat ttgaaactac
     4921 acctaaccct ataccagtta atacaggacc tatattatat tttgctcctc ctttacttaa
     4981 atcaactccc ttatccaaac atccatctac acaagtagat aaaaatggtc ttggtaccat
     5041 ttctttatga atttgttgag caacagtagt tcctataaca gaacttttta caagatatgc
     5101 taattgcttt aaaaactcat ccttaagttc ttcaaaagaa gtaaattctg tgatattttt
     5161 tgtatctaac cccatttttt ctccagttaa tctactctta ccttcattca tagcatattc
     5221 taaagctgct gcaaaattga tatttacggc tgaagtccac tgtcctgttt ttctaaaatg
     5281 aggaactaca caaccacagt tactccaatc tctagcatct tctgcatcat atccatcttg
     5341 taataacatt tgtgcccctg cactatcgct gtgtatagca gggaaaccag ttccttgttt
     5401 taccaattta gcaactgcca ttacgaaatt atctggtgct ccttgactca ctctaacact
     5461 taatcctggt tggtgagttt ttacactttc tgtagccttt aaacacatat atgagatatc
     5521 attcgttcca tcagtaccat ttctcttttt accaccaaca gtaagatttt ggaattgatt
     5581 atatcctgca aagtaatttg ctgtatttgc tgatatagtc catacccatt ctgataactt
     5641 aatccaaagt gcttctatca attcttgagc ttctgattca gttattttac cttctctagc
     5701 atcattttca taatatggat acatatattg gtcaaatcta cctaaattta atgccaatgg
     5761 attttctgat agaataccac ctaattgaac aaaccataca aattgtattg cttcataaaa
     5821 attagttgga gggtttgctg gtacattcat acatatctct gaaattttta taagttctaa
     5881 ctttctcttt tcatctgttt cttgcttact catctctttt gcaagatttg aatatctttg
     5941 tgccaatgtc ataataccct gtgcaactat agaaacagat ttgtaaaaat ctatcttttc
     6001 aatattttct gaaactgata tatctaattc tttaattttt ttatctgcat cttccttaat
     6061 ccctttatat ccttttacaa acaatatatc ttgataatct ggtgttactt caccaactgc
     6121 ctgtctccac ttagaatcat tatctattat tcctgtatct actaatatct tagctgtatc
     6181 ttctgggatt cttgctaaga aaacttcttc caaagatttt cccttccaat atggaaatat
     6241 ttcttttctt ataaattcta tttgttcttt tgatattaca tatgggtctt gagttctttt
     6301 atcaaaagta tccatttctt tgtctaccca ttgccaagaa aactctggtg tcaatatccc
     6361 tgttcttcta aattttcctg ctgtacctac tattaattca ttttcaaaga tttttatagg
     6421 aagttcttta catgtctcca aaaaagcttt agctcttctt atacagatag cttctccctc
     6481 agttttttta tgtgattctg taaaaattct agccctttca caacatatca taggtttaga
     6541 attaatatat tcttgtctaa atgcttctat tctattagtt tgactcattt aaaatccccc
     6601 tttggataat atatactaca tttttgtatg ttataacata ctttaattat attatactgt
     6661 cctttagcat aaaatggaag caaaataact tatttttttt catctacctt gaacacctat
     6721 atattaatac tttttttgtt aaatttataa actctaaaaa aattttctat gatatatttt
     6781 ctatattgtt atagttattt ctaatatact aatgatttga aatatcaaat ttgtatacta
     6841 gacttagttt taaaacctta gttgattgtt taactttgtt aacttcaaca tgtatatcct
     6901 tcatataagg aagctctaca acacctactc ccttattaaa gtcttcttca accatagaaa
     6961 aaatttcttt ctcagttaag ttcgtattag tcaatgactt aaataaattg tatataaatt
     7021 taatatattt atcactctca gataacttat catcttctgg atactcttta gcacagagaa
     7081 tatctattgt tacttttcct tctttatcgt cttcagacat agaatagaat aaacttcgaa
     7141 tatttttttc aggtggtttt tcagcaccat ttatagatac tacaaagcta ccatctgatt
     7201 ttgagtcagt aatactataa ccactgtcta ttatagcttt cgaaaatctt tgagataaat
     7261 ctttttttac ttcttctcta cctttcgcat cattaatctt ttgtgagtaa ttattaaaaa
     7321 gctcgataga ttcatctttt ttatcttcaa cttgttcttt caacgaatct ttactaattt
     7381 gttctgagct acttaagttc ttatcatata caggagacat acaccctacc atcaatgcta
     7441 agctaagatt taaagccaca atgattgcca ctctatttat attccttttt actttcatac
     7501 acaatcctcc catcacacta accgctctaa agtattactt attgcactag aatatgttta
     7561 tattttcttt atattaaaga tttattattc aatacatatc accttcctat ccaattctta
     7621 taacttacta ttagtaaaat tataagctcc ttaatacaga aataaggatg ttatcttttg
     7681 ttattaaaag ataacatcct tatttttttg tatcaataca tatgtcccaa ctggtaactt
     7741 aatcgcccca actgtcagta tcttggtcca atggtatata tatatttagt ataaatttat
     7801 cgatactatt ttcaaataat aattctccat tatatttttg taaagaagat ttaatactct
     7861 gaatacctat tccatgtaca aacttatcca ttttgtttgt taacaatata tttttattaa
     7921 aagtaagctg atttagttta ctattttcac atttaagtac aaaaaatgac cttgtcatag
     7981 ttcctttaat tctaatatat ttattaacat tttcatccac tattttatca catgcttcta
     8041 ttgcattatc caaaatatta gaaaatatgc ttgacacatc tataggtttt acaaaattta
     8101 cttttgaaaa atttatagag caagtaaatt taattccttt ttttaaacat atttcactct
     8161 tttcgttgat tataatgtct aatatcatat ttcctgtatt atagatatat ttaaatgttt
     8221 ttatttcatc ttttaaattg ttaacatatt cattaatttc tttgctattg tttctaaggt
     8281 tgtctataca ataaatgtga ttatttatat cgtgatatag tttcttaact cgcatatgag
     8341 attcttgaat acttaaataa tgcgcatatt gcatatctaa cttatttttt attaattcag
     8401 cttgagattt taatctattc tcttttatta tttctttaaa caatagtata agaaatatac
     8461 tactagctcc taccaatttt ggattaacaa aattatcaaa atagtatcct tctgaatgta
     8521 atgacccaaa ccgttctaat ttttttaata cataatttac aagcatattc attgtattaa
     8581 ctaataaggc aaataataaa tatacataat ttctcttatc ttggctaatt aattttatag
     8641 acttcctaaa aaagctaaat ataagatata taagaataat tattaaatta ctatatatct
     8701 gaatttttat agattctaag tgaggtacta ataaggattc tttagatgaa ataacattac
     8761 ctgtaattgc aaaaaatagt attctatata taacagcatc caaacattta taatatacac
     8821 taaatagata aaagaaaatg gaatacttaa tataacccat cttataaaaa taattatagt
     8881 atcctcccca agcaataaat gctaatatat aaaataaata gtttatatta ctaaagtaat
     8941 ttcctccatt aaataatcta cttatctgaa ttataatata aataagaaac aagacaatat
     9001 agaatactat tttttcattt tttttactat cctttttata aattaaaatt ccgttcatta
     9061 tagaaaactg tataaatata gataaaacta ctaatagctc agtcataaag tttagcatat
     9121 tgtatcacca agaaatttta agaatttctc cttaacttct ttatatctat atctactaat
     9181 aggtacctct tctccacttt ccagtattgc agtattaggc tttatatttt caacatatct
     9241 taaatttact ataaaacttt tatggcatct tataaattta tatggattta attctttttc
     9301 tattttgcct aaactatatt ttatatcaaa attcttgttt attgtgtgta ttagcatatc
     9361 ttttttttga acttctatat attttatttc atttaaataa atcttatatg tattagactt
     9421 attttttatt gtaatatgac tttccttatt tatttcaata tctttgatac aagttagcac
     9481 atgttttttt agttcttcca actctattgg ctttagtaaa tacctataag ctcttacctc
     9541 ataaccttct tgcacataat ctattaatga agttacaaat attatttcca tttcattgtc
     9601 tacttctcta atctttcttg cagtatccat tccatttaat ttttccattt ttatatcaag
     9661 aagtaatata tctatatctt ttaaattact ttccagtaac tcttctccag atatatactt
     9721 ttgtatctca tattctatac ttatatcttt taatatattc tcaaggtaat cttctattaa
     9781 aagttgtgtt tctttttcat cttcacaaac tgctatctta atcataaaat gcctccttac
     9841 ataatttcta gttaatacaa ttaaaatatt tataatatat ttaaagataa ttttgtatat
     9901 ctgatacttt aggataacat tttcaaa
//
LOCUS       DAEMGG010000138         9834 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEMGG010000138 DAEMGG010000000
VERSION     DAEMGG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.139"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            206..2218
                     /locus_tag="KPK24_003218"
     CDS             206..2218
                     /locus_tag="KPK24_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2300867.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            2587..3807
                     /locus_tag="KPK24_003219"
     CDS             2587..3807
                     /locus_tag="KPK24_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2300868.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAVVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            3844..5193
                     /locus_tag="KPK24_003220"
     CDS             3844..5193
                     /locus_tag="KPK24_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2300869.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKKGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFVGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(5325..5618)
                     /locus_tag="KPK24_003221"
     CDS             complement(5325..5618)
                     /locus_tag="KPK24_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300870.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
     gene            complement(6104..6307)
                     /locus_tag="KPK24_003222"
     CDS             complement(6104..6307)
                     /locus_tag="KPK24_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300871.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
     gene            complement(6358..6609)
                     /locus_tag="KPK24_003223"
     CDS             complement(6358..6609)
                     /locus_tag="KPK24_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBG2300872.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            complement(6625..7695)
                     /locus_tag="KPK24_003224"
     CDS             complement(6625..7695)
                     /locus_tag="KPK24_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBG2300873.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            complement(7724..9145)
                     /gene="ccpM"
                     /locus_tag="KPK24_003225"
     CDS             complement(7724..9145)
                     /gene="ccpM"
                     /locus_tag="KPK24_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBG2300874.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            complement(9167..>9834)
                     /locus_tag="KPK24_003226"
     CDS             complement(9167..>9834)
                     /locus_tag="KPK24_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2300875.1"
                     /translation="NLTLNKNDPIAILGKNGSGKTTLLKILLGFYDDYEGDILINGKN
                     LKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVYKSNLEEKVKSKLKETKLKPSI
                     YEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDADMYIFDEPDSSLDSEAGNELL
                     SLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKLLESGRHDELMDKKGVYFNLFC
                     NCK"
BASE COUNT         3723 a         1347 c         1183 g         3581 t
ORIGIN      
        1 atatacatac agtttaagtt ttaagcaaca attaggggga tttggtgaca agatttttaa
       61 ctataagaaa gaaagtgtct taatgcaact tttagaaagt cattatagac actttctttt
      121 ttatttgata aatttaaatt atcaaaatta gattgcttta tacaactatt ttgttggaaa
      181 ctatttatta tataaggaga aataaatgaa aaaatcagta gctctggtaa atgatagtag
      241 aaaagattta atagactttt tagaaaataa tttgaaatta gtttttggag attctataaa
      301 tattaataga tacttcataa atgaaataaa tgataatgat attataaatg atgatgtgat
      361 attagtaatg tcagtagaaa gattagacaa aattataaat aatatactag ataagaaaaa
      421 agtgattgtg gtaagaagaa cttttaggga agataaaatt tataatctac tatctttacc
      481 acagggtaca aatgttctaa tcgtaaatga ttctgatgaa actactctag aaactataag
      541 tttattttat aaaattggag tgactaatat tagaccaatt ccatatatga atgataataa
      601 ctataaaaat ataaaaatag ctataactcc aggagttcca gaaaaagtac ctagctttat
      661 ctcagatata tttgatttag gtcacagata tatagatatc tctacattta tagaaataat
      721 aaatttatta caaattgatt caaaggaaat acaatctaat ctagttaaat actcagaaga
      781 aataataagt ttagatactg gtataaaaga taaatataag gaattatttt taaaaataga
      841 agaattagat acaatcttga atttatcaaa agatggtata ttatttactt caaaagatgg
      901 tgaaataaat acatataata gtaaagtaaa agatatttta gacataaatg aggatatata
      961 tggaaaatac attgaggata tatttgtaga tagtttaaaa gttttattga gtgagaaaga
     1021 gatactagat aaagttgtcg tattcaataa gaaatatata aatgtaaata aaaaaaacat
     1081 atacaataga gatgaaaaaa tgggcacata ttatagttta caagaaataa catacataaa
     1141 aaaattagaa caaaatctca ctaaaaaatt aagagaaaaa ggacaaatag caaaatatac
     1201 atttaaggat ataaaaacaa atagtcctaa aatgtttgaa tgtatagatt tagctaagaa
     1261 ggtttctaaa tctgatttaa gcatacttat cagaggtgaa agtggaactg gtaaagaact
     1321 catagctcag tctatacata acaactcaaa tagaaaaaat caacccttta tagcagtaaa
     1381 ttgtgcagcc gttcctgaaa atttattaga aagccagcta ttcggatatg acaagggaac
     1441 ttttactgga ggtctaaaag atggaaaaca aggattattt gaacttgcta ataatggaac
     1501 aatattctta gatgaaatag gcgatatgcc tttagaatta caaacaaaac tacttagagt
     1561 tcttcaagaa aagcagataa tgcctgttgg atctcacaat gtaattaata tagatgttag
     1621 aatcatatct gctacaaata agaatttaga acaaatgatt gataatagcc aatttagaga
     1681 ggatttatat tatcgattaa atacaattcc aataaatata cctcctctaa gagaaagaaa
     1741 agaagacata ttaattatta tggaagattt aattaataag aaattagtaa tcacaccaga
     1801 agcaaaaaaa cttatacaaa actatatgtg gaaaggtaat ataagagaac tccaaaatgt
     1861 tacatcctat ttaaacatta tgtgtgaaga tattgtatta gagaaagatt tacctccaaa
     1921 cttaaggtct tcagataaca aaaatacatc attaaaacta aaatatagta agaatgatat
     1981 attgaatata ttagagatac taattttaaa taaggaatca gatgttggta ttggtagagg
     2041 attaatttta aaagcactac tcgataagaa ccttcaaatt acagaaggaa aaattaaaaa
     2101 aatatttgaa tatctaaaaa aagaagaact tataatttgt agttcaggaa gatatggaag
     2161 taaaataact caaaaaggag aagattttta caataaatta aaatataaag gtctataaaa
     2221 ttataattta tcaaacaaag aaatctattc aggattaaag tactccttca ataggttttt
     2281 ttatttttca agtaccctaa aattataatt tattttgtac ataaataata ggaattaata
     2341 ggaataggat aattttttta tttcctattt tttttatata aactagcata taaaatatat
     2401 tttcttcaaa ataatatttt ttactgaaaa ttgaaactat gcaatcattt tttatactat
     2461 acatatagat aaatgattat atttattcaa tattcttata aaaaacaaaa ttctccatcg
     2521 aaaattatgg cataataatt gcttattata ttaataaaac aataaaaagc aattggagga
     2581 ataaaaatgc aacaggcttt gaaattaaaa tatcaaacca aagaattagg aaaagatttt
     2641 gtaatagaaa gttgtaattc tataaaacca tggcttataa acataagaag agaattacat
     2701 aaaattccag aattagctct tgaagaaaat cttactaaac agaaggtaat ttcttattta
     2761 aaagaaatcg gaattgatta tatggaattt acaaaacaca atggaattat ggcatatatt
     2821 ttaaaagaaa gtgcagataa aacgatttgt ataagagcag atatggatgc tcttcctatt
     2881 gaagaagaaa acaacatacc ttataaatca atacattctg gaaaaatgca tgcttgtggc
     2941 catgatgcac atacaaccat gctacttgga gcatgtaaag ttttacattc aataaaagat
     3001 aaacttaatg taaatgtaaa attcttattt caaccagcag aagaaggttt tggaggagca
     3061 aaatttttag ttgaggatgg gtgtttagaa aaccccaagg ccgattatat attcggactt
     3121 catgttatgc cacatataga aacaggcctt atcgaaacaa aatatgatac attaaatgct
     3181 agtgtagaca ctataaaaat aagtatcaaa ggtaaaagag ctcatggtgc atatcctgaa
     3241 aacggtattg atgcaatagt tacagcttca caaattgtta catcattaca aactataata
     3301 agtagaaact tagaaccaaa taatgctgtt gttttgacta taggtaaaat atatggtggt
     3361 gatgcacaca atgtaatatg tgaagatgta aaattagaag gcactttaag aaccttaaat
     3421 agcaaaacaa gaaactttat gattgataaa atagctaaaa ttgttggaga tactgcatct
     3481 gcatttggtt gtgttgggac tttacatgta agtgatgaaa actatcctgc tgttataaat
     3541 gaaaaagaat tagtagatac agttatatca aatactaaag aattacttgg agaggaaaaa
     3601 tttatattga gaccaaaccc gtctttaggt ggtgaagact tttcttttta cacagaacat
     3661 tgtaaaggag cattcttcca tttaggatgt aaaaatgagg aaaagggctt aatttcacct
     3721 cttcatacct ctagctttaa tatagatgaa gattgtttac caataggtgt tatgatgcat
     3781 gtaatgaata ctttgtattt taattagtac agtaattaat aaaagaatag gagataaatt
     3841 aatatggaaa aagctaagaa attaggtcta tttagtatga tattattagg gattaatagc
     3901 ataataggtt caggtatatt cttactacca ggtaaagttt ataatctagc aggtcaaaac
     3961 agtatgttta tatacatttt tgctacactc ttagttctat caatactttt atgttttgca
     4021 gaagttggaa gcatgtttga taagaaagga ggagcatatc tatactccaa aaaagctttt
     4081 ggagatttta taggatttga agttggtaca atgtcttggg ttattagaat tatatcatgg
     4141 tctactctag cagtaggatt tgcaacagct ttaggttcat tttggccaga atctgccact
     4201 gaatacaaag gttatatagc tgcaatactc gtaacactac tttcaataaa tagcttattt
     4261 ggaattaaaa gcacaaagat aatgaataat gtaataacta tagctaaatt agtaccatta
     4321 atagttttta taatagttgg aatatttttt ataaaatttg taaatattgt tccttcagga
     4381 aatgtagtta actctagtat gggtccagcc attatattag tattttacgc atttaccggc
     4441 tttgaatcat ttattgtggc aagtggagaa atggaaaatc ctaaaaaaaa tcttccagtc
     4501 gcattaataa ccacaatatt tatatgtgca ataatttata tattaattca aattgtctgt
     4561 atgggtatac ttggagatag actatttgaa aacagtattc caattgcaga tgcttcaagt
     4621 gtatttgtag gcaattatgg taaagttttc atatctgtag caacattaat atctatattt
     4681 ggtataaata taggttcatc aatagtaact cctaaatgtg gttcttctct agcagaagaa
     4741 ggttctcttc ctgcatttat aggaaaaaca aataaatatg gtgctccata tgtagcaata
     4801 ataatatctt taatttgttg tataccactt gtgctaactg gaagttttga acaacttgca
     4861 gtaatgagcg taattgcaag atttgctcag tacataccaa cttgtttatc agtaatagtt
     4921 ttaagaaaaa gaacagatgt taaggcctct tttaagattc catttggccc tgttatacca
     4981 atagtagcta ttttaggtag cttatggttg ttacaacaag cttgggcaga agatatttca
     5041 aaacctataa ctcaaaatag agttttaata ggattagggg caatgttgtt aatagctcct
     5101 ctatatatat ttatgaaaaa aaataaggaa atcgagcata aaaataaatc agaagattct
     5161 aaattatata tttcgagaga aaaaattcaa taatatttta tactattact tctcataaaa
     5221 tataataaat ataaaaaaat tgtagataaa actattttat aaaatttatc tacaattttt
     5281 ttatataaac atatatcaaa atcaaaatat tattaattta tactttaaag attcccatct
     5341 ttatcatatt ttattcttat aagaaaatat aatcctatta ctactaactc tatcactaaa
     5401 aatactccat aggtaagaat tgaatcaata ctatctaacc catttctaaa aaaaattaat
     5461 gctatacctg ctgctataat ccctatagca ttttttctta ctgcattttg cccaactcca
     5521 taaaaacctg gcatttttcc ttttatttcg acagcagtta aaattatccc aaacactata
     5581 aataatactc caataagcat cataatcata ctaatcatct aattacctcc ttcacttaag
     5641 gaaatttcaa tttctaaaat ctaaaataaa taatatttat tttttataat aatattgtat
     5701 atccaattaa ctattttgga aattagtacc aataaataat gtttaaatat tgaaataata
     5761 tattaattct aatcaatatt attaatatat atatacattt ttacaaatat atttaaaaac
     5821 tttactttta attcttacta tttaaataag tataatttat tataataact tttaattaat
     5881 ttatactttt aaattgtaaa aatcacccct aatcactata tttaccacta taataattga
     5941 agaggtgatt tatataacag atactattta tgcagttatt ataaaatcac actcttaatt
     6001 ataatcaact gcatattttt ataataaaag gaaataaagt aagaaagata aatttaatca
     6061 aatatgctaa atcaatttaa tagtatactt attactgaaa tatttacatt ccatcaggag
     6121 ttctttggcc atgtatcaat ttattataat atgtattata tgatcctcct cttgcattat
     6181 aatcattatt cttgcagtta caagatgaag atgccatcat cttacaagta taaggacaca
     6241 taatagtaca aacacctctt gtctcaacac ctatattatt catttctatt ctttttccaa
     6301 aactcattca tatcctcccc ttttctataa gatcacttac tttatacctt tattaaccta
     6361 taccatattg attattttta gaacattatc aaaactcgta aattgcctat tcaaaagttt
     6421 ttcctctgga atttttatat taaagttttc tattacctcg caaaatacta aaagtaattc
     6481 tcttggctgt atatgtaaag acggtgaaaa aaataattct ttttttaaat tgttattttt
     6541 attaaaatta attccagtca catcaaaaaa tatattctct agtagtaatt ccttttcagt
     6601 gtaattcata attaacctcc aattctaaat agatattact ttagcatact cttttaattg
     6661 attcactata ttgtccacta attctagtaa ttgtttatta tcatgtataa aaaatgtttt
     6721 ttcataatct tctatatctt caacaaaaaa gttttcatct aatgacatat atgaaaattt
     6781 ttgcttcacc tctgtctcat atgtatctat aacttttttc tctatactaa aatagtctat
     6841 atttatacca tgtcttcctt taataaaata tttaagttct tcacaatatt ctctatcata
     6901 tatacttgat ggaatacata atataccaca atcatttatt actgcattag aaatttcaaa
     6961 agctctaatt ccaaaattac caaccatttt tctagagaaa gggaataatt ctcctggaac
     7021 ttctataatt attaaatctg gctttgcctc tacctcttta tcttttataa aatggttaaa
     7081 attaataatc tttgtcttac tacttatcga atcatcaaac ataaactctg gtataggaat
     7141 cattttaaat aaatagctct cttttttagg gccaaattgt aatattgaaa accccctttt
     7201 tcttagttct tctcctatcc ttatatttac atcaaaagta tctaaccctc cataaagacc
     7261 acaaataaat attattggtg tgtttatctg tgatataccc tctatatctc ttatttcata
     7321 tttttctaaa ttccattcat taatcttttt gaattcataa tgagatatat acctagatat
     7381 tttattaaga tcatcacgtg tatataatat ctttttacct tcttttagag ctacttttaa
     7441 tttaggaatt ataaattttt ctaagtctag attttctttt gaatcaacaa accataccaa
     7501 agaattcttt ttaagttcac tttgaaaatc atttgtaact ttagtaataa tattattaac
     7561 attatactcc ttattgcata acccccagcc agaaggacta accagtgaat taatttctat
     7621 atctgaaatt ttattgcaat gatttaaaat agtaaaaaaa ttataattaa atggatatat
     7681 aagtactttt tccataatcc catctttcct ttcattagaa aatttaatat tgatatccta
     7741 gagatttcaa aacgcagtaa tctttcatat tattttctgt agaatgtcta attctgtcac
     7801 aatcatgtgg ttttatttca ttttcattac atttagtatc atcaatctta gctatacata
     7861 tatcacaata actataggcc caacaatcag aacattgctc ttcatataat tttcctatat
     7921 ttaaaagttt ttcagccttt tctatatcta ctccactatc taaatttcca atcataccca
     7981 ttttagaact ttcacttact ctctcacatg gaaaaaattc tccatctgta gttacaaata
     8041 ttctaaaagc tcctggtata catggtccac catgatgcca cacatctggt aactctaatc
     8101 taccttcaat atttttctca tatctaaaat catctaataa ggctctttct attcttgata
     8161 aaggtgacat actctcatct tttataaatt ttaatttgtt gagtaaagtc aagaatctat
     8221 cataactctt acctaatata tattcatctg atgaaactat ttctttatca gaatagttat
     8281 tattaagagt ggaagctaaa aagtttgaat cttttaaagt ctcattttta gatataaaat
     8341 catctataga aatgtaatca ttttctgaat ttatcactgt attaaatgta atattctttt
     8401 tataatattc tggatattta ttttttatgt aacgtacatt ttttaataca acatcatgag
     8461 atccttctcc tgtatttgca aattttctac ttttatcatg aatttctttt cctccatcta
     8521 aactaatcat agttgttata ttatttttta caaagaattc tattatttcc tcattaaata
     8581 aagttgcatt tgtagttaga ttaaagtata tttcctttcc ttctccaact acttttacat
     8641 attctactat ctgcttaatc aaatcaaact caagtaaggg ttctcctcca taaaagccaa
     8701 cactaacttc atttaattga ctagaattct taatcaaata gtcaatactt ctttttgcta
     8761 catctatatt cattctttta tttgtatgaa ctctgttgct gtatgaacca gaataaatgc
     8821 aatagtcaca acgtaaatta caattttggg ttacttgtaa aataatagag gatagattat
     8881 tttctatatg atattttata tacttagttt cagggtgcat tgttttactt acccttttat
     8941 tttttagaaa tccataatta atcaaggtat taatttcgtc ttctatttct atgttccagt
     9001 cactttcttt ttctaaatta tgctcaattt tatttagata attataaaca gcagcattta
     9061 cctttaaaat cttatccgta ttaacatcat aaataaaata tgattttcca gctttaaatg
     9121 tgtgtataaa tggtttttga ttcattaatt aacctccctt taatatttat ttgcagttac
     9181 aaaataaatt aaaataaaca ccctttttgt ccataagttc atcatgtctt ccactttcta
     9241 acaactttcc attttcaaga actacaatat tgttagttat cttttttgca tgttggatat
     9301 ggtgcgttat aaatattcca atcttatttt caaatgtttt tttatataaa gaaaggagtt
     9361 catttcccgc ttcagaatct aaagatgaat ctggttcatc aaaaatgtac atatcagcat
     9421 ctttcataag agttctagat attgctactt tttgccattc acccatagaa agttgcttct
     9481 taccaaacca ggttcctaaa atagtttcta atccatcttc ctcataaata cttggtttca
     9541 atttagtttc tttaagttta cttttaactt tttcttccaa gttactctta tatacatctc
     9601 ccatggcgac attttctttt aatgtagcct catatttaat ataatcttga aacatacaac
     9661 ttacttttct aatgtattca tctttttcaa tatcctttaa attttttcca tttatcaaga
     9721 tgtcaccttc ataatcatca taaaaaccta ataatatttt taaaagtgtc gttttacctg
     9781 aaccattttt tcctagtata gcaatgggat catttttatt taaagttaaa ttaa
//
LOCUS       DAEMGG010000139         9798 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEMGG010000139 DAEMGG010000000
VERSION     DAEMGG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.140"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..855
                     /locus_tag="KPK24_003227"
     CDS             <1..855
                     /locus_tag="KPK24_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2300876.1"
                     /translation="SIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFEAVLEDKKNL
                     IFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAERYSFRITQI
                     KGYDIKNQIEYVESIKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEWTSNHYKFTL
                     CFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDEFLFLLQNTS
                     CVEAEKIMININYELSKISNTDEYPYALSISYGLVEWDGISDIETLISKADSLMYENK
                     LKNKSTLN"
     gene            1046..1213
                     /locus_tag="KPK24_003228"
                     /pseudo
     CDS             1046..1213
                     /locus_tag="KPK24_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(1473..4040)
                     /locus_tag="KPK24_003229"
     CDS             complement(1473..4040)
                     /locus_tag="KPK24_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG2300877.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSNP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRAIKSGQMITVDGLAGTVTFDE"
     gene            complement(4043..4648)
                     /locus_tag="KPK24_003230"
     CDS             complement(4043..4648)
                     /locus_tag="KPK24_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2300878.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(5022..6482)
                     /locus_tag="KPK24_003231"
     CDS             complement(5022..6482)
                     /locus_tag="KPK24_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG2300879.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            6836..7414
                     /locus_tag="KPK24_003232"
     CDS             6836..7414
                     /locus_tag="KPK24_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2300880.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYSNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            7465..7698
                     /locus_tag="KPK24_003233"
     CDS             7465..7698
                     /locus_tag="KPK24_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300881.1"
                     /translation="MIELIQRLNDIIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFLIILGIKVRKKFLIIIAIISIIINILIILDR"
     gene            7707..8957
                     /locus_tag="KPK24_003234"
     CDS             7707..8957
                     /locus_tag="KPK24_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2300882.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            9118..9687
                     /locus_tag="KPK24_003235"
     CDS             9118..9687
                     /locus_tag="KPK24_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG2300883.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3255 a         1704 c         1471 g         3368 t
ORIGIN      
        1 tctattgcaa atatttacag agtatttcct atatcattta gctttatagt tacaggacta
       61 tatttcagtt ataaaaataa taaaaataat aagattaact ttgaggcagt tttagaggac
      121 aaaaaaaact taattttctt attgagcctt atgtttggaa tgtacttgta tctatgtttg
      181 agtttgtcaa tatactatac tgttggaaat gtattgattt taaaattata tggaatcaag
      241 tctgccataa tagttttggt aggtaactat attgcagaaa gatatagctt tagaattact
      301 caaataaaag gttatgatat taaaaatcaa atagaatatg tagaaagtat aaagacagag
      361 tatgaaatta ataaattgaa tgatattgta tatacagatt tactgacagg cttctacaat
      421 agaccttttg taaatcaaaa actatcagag tggacatcaa atcactacaa atttacctta
      481 tgttttactg atttaaatag cttaaaacct gtaaatgata aatttggaca ccaatttggt
      541 gataaataca tatccactac tgctcaaata ataaaaaaac ttataaatgt agaaaattcc
      601 ttactattta gatatggtgg agatgaattt ttattccttt tacagaatac aagttgtgta
      661 gaagcagaaa agataatgat taatataaat tatgaattat caaaaataag taataccgac
      721 gagtatcctt atgctctttc cataagctat ggattagttg agtgggatgg aatatccgat
      781 attgaaactt taatatcaaa ggcagattct ttgatgtatg aaaataagct taaaaataaa
      841 tctactttaa actagctagt tcagatattc tacaaaatat atattataag atagtactaa
      901 gaatgtcaaa atatatagat tttgcaaata ttgaatacag tttactaatg aatgcactgg
      961 atgtaagtgt tagtaaacac ctattagatg aacactttac tgttgtttgg gctaatgacc
     1021 gttattatga aatgttcggc tatacctagc taaaaagtta aaaatgacca ctgtagccga
     1081 gggaatagag actagttttc aaaaagattt cttgcaagaa atagactgtg atatgttaca
     1141 aggatacgtg ttctctagac ctttaccaat aaaagacttt gaaaaactta tgtttcaaaa
     1201 ctcctccaat taacaatata aaattgctta ttataagtga tattattgta tcaacaaata
     1261 aaggaattgt ctcaaaattg attttttcta atcaaatttt tattttgaga cattctcatt
     1321 tttttgcata agaaaatacc caccaagcta cggtttttct taccgctgtt cggtgggtat
     1381 tagtagtatt atgaaatctg aaaactttaa gtattactac ttccgctagc gcaatcacaa
     1441 aggtcgaaag cttcaaagcg aaaataggct ttttactcat caaacgtcac cgtacctgcc
     1501 aacccgtcca ccgttatcat ttgtccgctc ttaatagctc gtgtggcaat gtgtgtagcc
     1561 ataacggctg ggatgccata ttcacgtgcg acaatgcttg aatggctaag aggaccacca
     1621 atgtccgtta caatcgcact tgcagatgaa aataatggtg tccatgctgg agttgtggta
     1681 actgcaacca aaacacttcc aggttgaaag ctttcaaaat ctgctggact attcagtaca
     1741 caagctggag ccgtcacaac accagtactg gtaccaatgc ccgtcaacac aattttccca
     1801 tctttctgct tctgttgtgc ttgtgatata gtttttttgt ttttctctgg caacttggat
     1861 ggtgacacat aacccatgta ttttttaagt tcagcttttc gaacaggtat tatcccgttc
     1921 atatcagata gagacttgtt tttatccaac tgctctataa gtacctccaa ttccgatttt
     1981 gtgagccaat aaatatcatc taaatgtgaa atagctcctc ctcgtatgaa tcgttctgag
     2041 atttcctgaa acatacaacg aatcagagga tgtcccattc ccatcaaata aatagcattt
     2101 tcacgcatag gagaggtttc ctgtgcccaa tggagcaact taaaaaatag ttttttacgc
     2161 aatccaccta tgtgttgtaa aatctcttct tctgcatgtt ttctacgctt ttcaaacgtg
     2221 gtctggcgta aaaaaggact ttcccctttt ccttccacaa atgtttttac ggattcaaaa
     2281 gttggtgtaa gtgtttcctg cggtgtggaa tgcgcaaaat caaactcata agcggtacga
     2341 ccaaactctt taaagtactt attgattcga tttttccatt ctatccatac ttcctgtgaa
     2401 actccaacag gcacaactga agacatgaaa tcttctgcta tctttattgc aggactgttt
     2461 tgcagataaa ggttgatgct gttattttgc tttacccatt ctgaaatacc ccacaactcc
     2521 ttttccgatt gcagaacaat cgtatcaaac ccaagcagga aaacggaagt atccgttata
     2581 ccagcacgtc gaactgctcc ttgaaataat tttgtaaata atgtttcact gatagaagca
     2641 gcaggcaatg taagctgaat ccttgtgaaa taaatacatg cggcataaaa tattgtctga
     2701 ataccctcca taatttgatg ggcgttcata gtatgcaggg gcttttcttc ccattgttta
     2761 atagcatcct catatccttt tcgtgccgtt tcccagcgtg caacgctgtt cataagtgtc
     2821 ctacgtaaag agcgtgggga aagggatttg atagcaatca aaagtggatt tgatttagca
     2881 gaaagataaa catatccatt aataaccgta tatgccccgt tttttggtaa taattttttc
     2941 gcacttttac caaacatatc tatccataaa agctctgatg cacggtttac gatttcaaga
     3001 ccaagcgtgg cgaacaaagg tgtaacagga tttggcaaat gttctgccaa acttcccttt
     3061 gtgtatacaa catcctgttc tggcaatacc cattctggtg gcagaacagt aattggacga
     3121 gcctgaacaa tgtacaattt atctttttcc agtgcccact ctacatccat aggcatttga
     3181 taatacttct caattttctt tccaagctgt atcagttgca ttacctgatt acgagtaaga
     3241 gcatattttt ttctcaacct ttcaggaacc atggtttctt ctgttccttc tgaagtacga
     3301 accgtcataa tctctttatt tgccacttca tacgatataa ttcgttcgga gtccttatcc
     3361 actacgattg tatcgggtgt aactaagcta gaaaccaccg cttcaccaag cccccatgca
     3421 gcgttaataa tcatttcgct acgtctacca ttgattgggt ttaacgtaaa cataatacca
     3481 gatgaatcgg aaaatgcaag cttttgtact acaacagcaa gtgcgacaat ctcgtgcttt
     3541 atattattct tcacacgata agcaatagca cgagctgtcc ataaggaagc ccagcatctc
     3601 tttacagagt caataacttt atcttcacct tgtatgttaa gataagtttc ctgctgacct
     3661 gcaaaagaag cgtcaggtaa atcttcagca gtcgcagacg aacggacagc caccgatatg
     3721 ttccctaatc cagcataagc cattttaatt gcgtccgaca cttcctgtgg catttctcca
     3781 ttatggaaaa gctcccctat tttcttagat acatcttcaa gttggctggt attattggaa
     3841 tcaataccat ctagcagttt attaattcga gactgaatat ggttcgtctc aacaaaaatc
     3901 tggtatgaag cagtcgtaac atgaaaaccg tctggcacag ggatgcctgc tgtcaatagt
     3961 tttgataaag acatcccttt tccaccaaca atttcaatgg ttgcttgttt atgttccagt
     4021 ggcaagacat aataaatcat aatcattcac ctccatcatt cataattccg tgcagaaata
     4081 tattcattgt ttcatccaac attttttccg aactatccaa tggtcgtgac gtgtagctga
     4141 ttgataacat ggagttaatt aacaaacgtg tcacaagagc ggtatccgtt ttgcgaaaga
     4201 tgcctgtggt cacaccatta tcaattaccg attttataat atcttgatat gcataacgag
     4261 catctttaag tctctttcga tactcttctt ctaaataatc agattctgta tttaaccaca
     4321 tcagtacagt tctatactgc tctggaaatc taagatgatt aagcatgatt tttctaaggc
     4381 actgttgtgg tgaaggttca ccatcaataa tcctatgaac ttgttggata gttttttcta
     4441 tttctttctc aactgcatat acaacaatct cgtctttcgt tttgaaaaaa tcgtatagac
     4501 ttgactttcc catgttggct aaaactgcaa tttctctcat tgatgttttt tggaatccat
     4561 tttcagccat aagactcaaa gcaacagcaa ctattttttc tcggcgttct gcttgttgtt
     4621 cgggtgtaag cacaattcct tttggcatag ggcgatacct ccttttagcg accactggtc
     4681 gtttcttttt tatttatcat acagcgacca acggtcactt gtcaaggtta atttttttca
     4741 ttgtatatgg tactcgagat aaaaatgttc attttagaaa ttgctctatt taagtagaaa
     4801 taggcttcat cattagaaaa caagactata aatttgagat atttatcatg ttcaaatttc
     4861 atcaatatca aagtttaaga tattaataaa aaaagaatta ctaagaaata acatataaag
     4921 attcaaaaaa ggagttgaac ttaaaattca attacaaaaa atcaaattta agacaactcc
     4981 tttattcttt atctctacta ttctttatct ctatttattt attacaactc cctaacattt
     5041 accactactg ccttaggtct agtcatagcc tcgattgaat acttaacacc ttgagttccc
     5101 ataccagatg cctttacacc taaaaatggg aagtggtctg gacctctttc agtcttatta
     5161 tttatttgaa cagtaccaac ttcaagttta tcagctatat aaaatgcctt atcaatatca
     5221 cttgtaaata ctgatgattg aagcccatac tctgattgat tagcaatttc aattgcttga
     5281 tttatatctt taactcttat aattggaagt actggaccaa atggttcttc ccaagcaatt
     5341 ttcatatcaa catttacatt atctaacaat gttggataga ataaattatt ttttctaaca
     5401 ttgcctgtta ttaactttgc tcctttatgt aatgcatcat caaccaaccc ttgaacaaaa
     5461 tcagttgctt tattatctat aagaggagtg ataactactt catccattgg atttccaatt
     5521 gtcaacttct ctaccaatgg ctttattttt ccgactaatt tatccgccac actctcttgt
     5581 actaaaattc tctttactgc cgtacatctt tgtcctgaat atgaatacgc tccagatact
     5641 atattcttag ctgcaaaatc taagtctgca tcttctaata ctatagccgc atcttttcca
     5701 ccaagctcca ataataatgg taccattcca gatattttag atatatgctg accaacttca
     5761 gtactaccag taaagttaat aaaattaatt cctttatgcg ttacaacata atctcctatc
     5821 tcactacccc tacctgtaat agtattaagt actccatctg gtatcccagc ttttctcata
     5881 atttctacca catgaagtgc gctaatagcc ccttgtgtag caggttttaa tacaactgca
     5941 tttccaccaa taagtgctgg agcaatcttt gacattgata aatttacagg ataattaaaa
     6001 ggagatatag ctaaaacagt acctaatgga acccttgaaa cataagacat cttatttctt
     6061 gttccaccag ggaaattatc tccactaatg gcttcaccct ccatattttt tcccacatca
     6121 gctgtgtatc ttaaaaaatc tgctgttctc tcaacttctg cccttgcaga ttttatgtct
     6181 ttggctattt caagtaccag tatattagct atctcatcta tattttctaa caataaatca
     6241 gcagccttat gaaatatatt ggctctctta tatacaggca tttcagccca tacttttata
     6301 ctctcttttg tgttttttat aacctcatct acctcatgtt ttgtcataga ttgtactttc
     6361 ccaacaagag aattatctat tggtgagtaa atttctattg gctttctcga attagattct
     6421 acccattttc catttattaa atttttatat acattttctt ttgttttaag ttcattaaac
     6481 atatttttat tcctctctct gatataactt tgtgtattta aattatagcc atttattcgt
     6541 tatgtaaaca tttctttttt tcataaacgt ttacctataa aaaaatttta agttaacgtt
     6601 tttctacatt cttctatttt aataacatac tcgctccaaa tctatatagg tcacacttta
     6661 atatcaatat actaatatac caatatacca atataccaat ataccaatat accaatataa
     6721 atattataaa cataaatcac ttgacaaatt tataagtaga caatattata tatctatgac
     6781 ttaaaatatt tttaaagtca tagatatcat atatagatag aaaggattga taaaaatggc
     6841 taaaagtttt acagaaatag aaaaagacaa tataagaaaa agactaattt cagaatgtga
     6901 aatcaactgg tctaaatatg gatacaaaaa aactaatatt gatgaacttt gttcaaaatc
     6961 aggaattgca aagggttcat tctacacatt ctttgattca aaagaaaagt tattttttga
     7021 agtattaaat ggaatccaaa acagacttat atcatcagta gatgaaattt taagtaacat
     7081 gcctccaaaa gaaggctttg caaaagcctt aaagatgttg tatcgattat atgataaaaa
     7141 cccattctta tattcaccaa ataatactga ttacatcact ttattgaata agatacctaa
     7201 agagatgctt tcacaacttg aatacagcaa tgtacaaagt ttttatagct taattgaaag
     7261 acataatctc aagttaaaaa tagagaaaga aaaagccttt ggtgtttgca atgccctact
     7321 gtcaagccta ttgctaaaag aagatattgg atatgactac attgaagtat ttgattttat
     7381 ggtagacaat cttgtagaac acatacttga gtaataattt aaaataaatt ttctaaacat
     7441 ttctttataa ctaggaggag taatatgatt gaattaatac aaagactaaa tgatattatt
     7501 tacaaaataa atgataaact aaatatccac acaaaacttg tagaccctag cagtgagctc
     7561 atgccacttt actctatgtt atctataatc gtttctatat ttctaataat attaggtatt
     7621 aaagtaagaa aaaaattcct tataataatt gctattatat caattattat aaatatttta
     7681 ataatcttag ataggtaggt acatatatgg taaaatcctc gaacactttt aataaaaact
     7741 tcaacttgat gatattaggt caaataatct cattgtttgg tgcatcaata cttaaatttg
     7801 ctttatcttt atacatactt gatataactg gtaaagcaga aatatttgct actatactag
     7861 cagtatcttc aatcccaatt atagtattct cccctattgg tggagctatt gcagatatat
     7921 tcaacagaag aaacttaatg gttatttttg atttttcaag tagtcttaca gtactaatct
     7981 tagcagtatt cttattcaac aataatggtt caatattact tgtaggaatt attatgacca
     8041 tattatcagt cataagcaca atgtatcaac caactgttca gtcaagtaca ccccttttag
     8101 ttgataatga gcatttaatg aatgcaaatg gtattgtagc tggagttgca tctcttacaa
     8161 atattgcagg acctgtatta ggaggtgtct tgtatggtgt gattggaatt aatgcaatta
     8221 tagttataag ttgtgtaagt ttctttctat ctgcaattat ggagatattt atacagattc
     8281 cttttatcaa acaagagcaa aacagcaaca ttataaaaac tatatttact gatataaagg
     8341 atggtattgt tcatatatgt aaagaaaata gatttatact aaaaatactt tttttagcta
     8401 tagcaataaa cttattttta agctctatgt taatagttgg tatcccctat atagtaaaaa
     8461 tcactatggg agctagtaac ttcttatatg gtctagcaga aggagcaatt tcttttagtt
     8521 cactggtagc tgctgttagt attggtattt tctcaaaaca tctaaaaatc actaatttgc
     8581 atatttcctt tattatatgt gcagttatgt tcataccaat ggcggtttca gtatatccat
     8641 ttatattaaa cacaggattt ccagttccat ttttaatctt tatagtatca tctatgctaa
     8701 tattttttac aacgactctt atctcaatat atgttataac agtcacacaa ttaaaaactc
     8761 caaatgaact cttaggaaaa gtaatgtcaa ttctttttgc aggaacagca attgcaatgc
     8821 cccttggtca ggttatgtat ggtaaacttt ttgatttatt atcagataga gtgtacatca
     8881 ttatactagg agttggattt attacattac ttatagcatt tgtatcaaag ataacattga
     8941 ggaacgaaaa tctttagata caaagaattt agatataaag atttaatttt cactaatttc
     9001 tgaatttaaa tgcaaaattg tgtatcagta tgttgacgtt ttagaaattg attgatataa
     9061 tcaatggaaa ataaacttta taaaaattca aaatattcag ctagaggagt gataaaaatg
     9121 agttacatgc caactttaga acaagcatgg gaaatattaa gaaaatacaa taaggaagaa
     9181 tttcatataa gacatgctca aattgtatct ggagttatga gatactatgc aaaggagtac
     9241 gaccctgaaa gagtagaatt ttgggaaata gttggacttt tacatgattt agattttgaa
     9301 atgtatcctg atgaacattg tgttaaacaa agagaattaa tgacagagct tgatttagat
     9361 aaatcaataa tcgattctac aataagtcat ggatatggat taactggttc agatgttaaa
     9421 ccagagcaat ttatggagaa agtattattt gctgtggatg aacttactgg tttaattggt
     9481 gctgctgcaa taatgcgtcc ttcaaagagc gtttctgatt tagaattaaa atctgttaag
     9541 aagaaattta aagataaatc atttgctgct ggctgttctc gagacgttat aagaagtggt
     9601 gcagaaatgc ttggatggga acttgataag cttatccaaa caactataga agctatgaga
     9661 agcttgattc cagagatggc aatatagctc ctccatacat ataccataga agttacatct
     9721 ttaaagagca tttctctttt tgatgcaact tctttttata ttttgtagaa taaaaagttt
     9781 aatattttaa actcacta
//
LOCUS       DAEMGG010000140         9750 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEMGG010000140 DAEMGG010000000
VERSION     DAEMGG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.141"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            182..1150
                     /locus_tag="KPK24_003236"
     CDS             182..1150
                     /locus_tag="KPK24_003236"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG2300884.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            complement(1267..2397)
                     /locus_tag="KPK24_003237"
     CDS             complement(1267..2397)
                     /locus_tag="KPK24_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300885.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(2550..3503)
                     /gene="msrA"
                     /locus_tag="KPK24_003238"
     CDS             complement(2550..3503)
                     /gene="msrA"
                     /locus_tag="KPK24_003238"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG2300886.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            complement(3627..4532)
                     /locus_tag="KPK24_003239"
     CDS             complement(3627..4532)
                     /locus_tag="KPK24_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG2300887.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFRLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            complement(4903..6495)
                     /gene="hcp"
                     /locus_tag="KPK24_003240"
                     /gene_synonym="priS"
     CDS             complement(4903..6495)
                     /gene="hcp"
                     /locus_tag="KPK24_003240"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG2300888.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDICGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(6555..7280)
                     /locus_tag="KPK24_003241"
     CDS             complement(6555..7280)
                     /locus_tag="KPK24_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2300889.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSMSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            7445..8149
                     /locus_tag="KPK24_003242"
     CDS             7445..8149
                     /locus_tag="KPK24_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2300890.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYTKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            complement(8313..9584)
                     /locus_tag="KPK24_003243"
                     /pseudo
     CDS             complement(8313..9584)
                     /locus_tag="KPK24_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         3241 a         1657 c         1144 g         3708 t
ORIGIN      
        1 acatataata ctttaatatt cactaataga gagtatatta acattgcttt tttaaaaatt
       61 ttagtataaa atcatattaa ataaattttc tgaaataata gaatattaaa atattcaaaa
      121 acagctttta ataacatcta taattttcaa tgtatcgtta ttttaggggg agtgatttaa
      181 aatggcaata aagccaagaa aaatatctat tgtaggttca gggcatgttg gttctcattg
      241 tggatttagt ttaataactc aaggagtttg tgatgaactc tttatgattg atatagatga
      301 atctaaatca aaagctcaag ctttagattt agctgatgca gtttcatatc tgccacataa
      361 agtacatata gaaaaaggaa ctttttctga ctgtaaagat tctgacatag tagtgataag
      421 tgttgcagac tcttcagaag ggcctttacg tagacaaaac acaactagat tagatttact
      481 aagaccaact attggaatga taaaatctat agtaaaaccc atagttgatt ctggctttga
      541 tggtatattt gtagtcatat ctaatcctgt tgatgtagtt acaaattata tttgggaaaa
      601 atctggcttt cctaaaaaca aagtcattgg aactggaaca gctttagatt caacaagact
      661 tagaaggatt ttatctgaag aaactggaat cgctcaacaa tcaattcaag cttattctat
      721 gggtgaacat ggagattctc aaatggttcc ttggtctcat gtttcaattg gtggtaaacc
      781 aatcttagac atgataaaag acaacccaag tacttatagc aatttagact taccatcaat
      841 agttgaaaaa aataaaaaaa caggaataag tataataaat ggtaaagatt gtacagaatt
      901 tggtataggt actgctttag tagaaattgt aaaagctata ctacacaatg agaaaaaagt
      961 tcttccagtt tctacattac tagaaggtca atataacgaa agaaatgtat ttgcaggtgt
     1021 tccatgtgtt ataggaaaag atggtattga agaaattatt gaaatcaata tgactgaata
     1081 tgagcaaaac gaatttaata aatcttgttc tgttttaagg gaatatattg cactttcaaa
     1141 aacactttag attcaatatt ctttcttaca ctaaaagcag gtaaatgact tattgaaaag
     1201 acttttaact aagttaatgt ttaaatcatt tgtacctgct tttaatttta atactaaaat
     1261 ataaacctat tttttaccat caaattttat aactttactt tttttattta cctcttgaga
     1321 taacaatcct tttttcaatc ccatcaataa ttctaagtct tctggataaa gatttattcc
     1381 aaattcctct aaacttgata ctatactatt tacactcatt attttatttt ctatcaataa
     1441 ttcaactgct ctttttaaat acgttgggct tgaacctttc attttgtcta gtggttcttc
     1501 tatcaaccag ccttttttat ccatttcatt cataagataa ttgtattttt tgtaatttat
     1561 cttttctaga ttatatgctc taaaaatcat agcagatata ggtacaatcc attttttctt
     1621 tagttctaca taataatcta aatcttctgg atgagtcaaa tcttttaaaa aactctcttt
     1681 tggcaataaa aatgcacagg catactcatc ttctgaaaat tgcttacttg gtattcttaa
     1741 ttcacttgat attatatatc caagctcaca tgccaaagta taatttctaa ttgatgctga
     1801 ctttttgtca ttacccagag atattagata tttagattct ttactaattc tctgtttttg
     1861 agtaaagata gttgcccctt ttctatctac attcatacct gatattataa tccctttaga
     1921 ctctaacaaa cttaccatat ttactattgg agtactattt aaatcccaaa actctctagt
     1981 cttatatgca agagattcta tatcatttat attaatatct gacattactt ttctatttgg
     2041 caagttttct tttggaaact taatataatt ctccataaat gaatatattt tatgtattat
     2101 aattattttt tctctataag aaatctcttc tactctaggt agtttagatt gagggttaaa
     2161 gtggctatcc tctacaacga tatttatatt ttcattttta taaaaatact cttttggaaa
     2221 atttaatata ttagataatt ttaatgtatt ttctatattt ggtacatact tattttcctc
     2281 aaaagctttt aaatcttttt tatttatttt agtttcttta gacaactgtt ctatcgtctt
     2341 tgctctaaaa attctagctg attttaatct ctttccatta aagtctttta aattcatttg
     2401 ttttctctct ttcaaaatta aattcttaat ctattggtat aaataagatt acaattaata
     2461 atttatcata ctattgacaa aaaaacaaaa ggtgaaaata tttttttata ttttcacctt
     2521 ttagataagt ttataataaa tattcctaat tactcaaaaa gttttaaata ttctccatat
     2581 cctaaagctt ccatattttc tcttggtata aatttaagag aagcagaatt tatacaatat
     2641 cttaaacctc caagttcttt tggaccatca tcaaatacat gacctaaatg agaatttcct
     2701 ttagcacttc ttacttcaac tctgtacata ccttgtgaga aatcacttct ctcaatgata
     2761 gatttatcta taactggctt tgaaaaactt ggccacccac atccagaatc aaatttatct
     2821 tttgaagaaa ataacacttc tccacttaca atatcaacat aaattccctc tttgttatta
     2881 tcataatatt cattttcaaa aggtacttct gtcatatcat tttgagtaac ttcaaattgt
     2941 ataggagtta aagtcttttt taactcttcc ctatttatat tgtttccatc ccaatgactt
     3001 tttacaaaat tatatcttcc agacccttta taatacatat tgtagtttaa tggtcttttt
     3061 ttataataat cctgatgatt ttcttctgct tcgtagaatt tagttgctgg aattatatca
     3121 gtcactattt tactatcaaa tattttacta ttttccagat gttctcttga ctctaaagct
     3181 atattttttt gttcttcatc atgataaaat atagcagtct tatattgagt tcccctatca
     3241 ccaaattgtc caccatcatc tgtaggatta atctgtttcc agaagatatt tagaagttca
     3301 tcataactta ttatatcatc atcatatgtt atttgaattg cttctaaatg accagtagtt
     3361 cctgaacata cctcttgata tgtagggttt tcaacatgac cacctgtata tccagaaact
     3421 actttaataa ctccatcata cttatcaaaa ggttttacca tacaccaaaa acatccacct
     3481 gcaaaagtag caagttttat cattttcatc actccttaaa taaattatac tttatttatt
     3541 ccctaagaag taacttaata atctgcattt tattattttt acctactcaa tgaaatcatg
     3601 tgtaaaaata caattgcaaa ttgaatctaa tttctataaa agctcttttt ctttatccat
     3661 ccaaaagcat cttttatatc acaattttct cctaaagaca ttagatattc taatgcatca
     3721 caatcatcca tagctacatc tttttccatc ttaggattta atctatagta attatctcca
     3781 agaagttttt gagaaaataa ctgactcatt tgagaatttc cttctaaggt aatagatata
     3841 attggtaaat caggttcttt attaattatc cagtcaatag ctccccattc ggtagtatct
     3901 gcctttatac tgttatatac atatccagtc cctattgaaa aaagtcttgt attcttaagt
     3961 ctaaatccac tatcaatggc atgaattata cacgctaaac tcgggtcagt tgctattatt
     4021 cctccatcaa catgacgatt atatgttgga aaaaataccg gagcagcact acttgccatt
     4081 gctacatcta caactttgct agtctttgta aaagaatttg gcatattgtt ataaaatact
     4141 ggtttccatg cattttcctc atttcctata taaaaacttg gtatcattac taatttattt
     4201 aaatctttta attctagttc ctctggaaat atacttaata aaacttcttt taagtttttg
     4261 ttttcatact ttggtctgag tatttctgca taacttttat taaaaatata ctttgaattt
     4321 tcaattgagt aaagttcttt tatctcttta gggcttacac catacgcaag ccctagtgca
     4381 attagactcc ctgtagatgt acctccaaac atatttatat tgtttaatag atttggatac
     4441 tcttttacta tcctctcaag tatggatata ctcaatgccc cttttaatcc accaccatca
     4501 aatgccaata tattaaaatt agaatttttc atatacatat acccccacat aaaacacctt
     4561 atattttaaa ttttaatcaa aataatttca ctatagtata tatattttat gcaagttaca
     4621 tattacaatg ttcctctttt aatatttaag ttacattcaa acctttaata cttaaaataa
     4681 aatatataag atattatata tctaagtatt ataaatttaa tacaaaaatt cttaaaagaa
     4741 tatactgaaa gcaggtttat ttttcaatta ttacaaaaaa taaataaagg gtcatctcaa
     4801 aattgatttt ttaatcaaat tttaagttta accccttatc ttaattgtgg catacttaaa
     4861 aatattttaa ctataaatat ctttaagtat agatatattt atttatccta aaatcttctt
     4921 catatcttct tcaggagtag atataggtgt caatgcaaat ttatcaacca atatatctaa
     4981 tacattctta gataagaatg ctggcaaaga aggccctata tagatatttt tgatacctaa
     5041 atgaagtagt gtaagaagta tacaaacagc tttctgctca taccatgaca gtaccatgct
     5101 aagaggcaaa tcatttactc cacattcaaa agcatttgcc aatgaaactg ccactttaat
     5161 tgcactataa gcatcattac attgtcccat atccattaaa cgaggtaagc cacatatatc
     5221 cccaaggtct aaatcattaa atctatattt tccacatgca agtgtcaata taacagtgtc
     5281 tttaggagct tgttttacaa attcagtgta ataatttctt ccaggttttg caccatcaca
     5341 acctccaacc aataaaaagt gtcttatagc tccactcttt acagcatcta ctactttatc
     5401 ttgtacagat gaaactgtac ctcttgcaaa accagtagtt aatgtatctc ctccattaat
     5461 accagtcata tgaacatctt ctctatatcc tccaagttca agagcttttt ctattacact
     5521 agaaaaatct ttatcttcat caatatgaac catacctgga taagcaacta cttctgtagt
     5581 aaatactcta tctctataac tatctttaac aggcattaaa cagtttgttg taaataatat
     5641 tggagcagga atattatcaa attctttttg ctggttttgc catgctgtac caaaattacc
     5701 ttttagatgt ttatattctt ttagttttgg atatgcatgt gctggtaata actcaccatg
     5761 agtatatata tttataccct tatctttcgt ttgttctaat aatttgtgta aatcataaag
     5821 gtcatgtcct gtaactacaa taaatggccc tttctcgatt ttcatagata cctgagtcgg
     5881 ttctggtgtt ccataagttt cagtatttgc tctatcaagc aattccatac attttaagtt
     5941 tatttctcca gttttcataa ctagacctaa aagttcgtca atagacaagt ctcctccaac
     6001 tgctattaat ccttcataga aaaattcatt tacgtctttg tctgtataac caagtaccat
     6061 tgcatgatat gcataggcag ccatacctct taatccaaat aagattaatg attttaatga
     6121 acgtatgtcc tcattatcat tccataaatc cttcatatca tagttatcat ttctattaca
     6181 agttgttgaa caatctgaac atctaggtac aagcttactt ttttctacct cagttttttc
     6241 aatttgtctt tttatagctt catcatcaaa acttacattc gttatagtag tgaatagccc
     6301 ctctatcata attttatctg tttcacttgt tggtctattc ttatttccaa tagttgctct
     6361 tgaaagacca atcaacactc ctgtaagttc gtcttgtaag ttagctgtac cccccttttt
     6421 accacagaca ccatttatag tacaaccttt acctcctgct gtttgttcac attgaaaaca
     6481 aaacattttt tccatttttt attcctccct ttttatttat taaaatttat tatatttttc
     6541 tataattcac ttaattaaat ttctccatct acaatttttc catctacaga tatagtaact
     6601 atttgccatg gtatcatttt tccactattt tgtagagctg tcttaacagc atttactata
     6661 ccaccacagc aaggaacttc catacgtgtt actactatat ttttaatatc attttctttt
     6721 aaaatctctg taagtttttc agcatagtca acttcatcaa gtttagggca tccgattaat
     6781 gtaaccttat ttttcataaa tttattatga aaatttccat atgcatacgc tgtacagtct
     6841 gctgctatta aaagacttgc attctttaaa tatgatgcat tagttggtac taattttatt
     6901 tgtactggcc attgattaag ttgagaacct tttatctcta ctatatcatt gcttatagac
     6961 atactatttg aaacctctct atttattgac ttagactgag aacctggaca tccacatgct
     7021 aaagttttct tttgtgcttt ctttgcttcc atatttgctt ttactgcagc ttcatcatat
     7081 tctgctgctt ctctatactc aaatgaaatc gcatttgttg gacaagttgg cagacaatct
     7141 cccagcccat cacaatagtc atctcttaaa agttttgctt ttccatttac cattcctata
     7201 gcttcttcat gacaagcttc aacacatagt ccacaaccat tacacttttc ctcatcaatt
     7261 ttaattattt ttctcatcat ttatatcacc tcataaaatt tttttaattt aaagtattga
     7321 tttcttatat atatattttt aatacctata tttctttatt tcaaatttct tgtagtagga
     7381 tttttatatc cccttgcttt tataaactta gtatagtaca atgaaaacaa attatcggtt
     7441 gcatatgcaa caaggaagtg attatttgaa aaaattttta gaaaagcata tagacctact
     7501 taaaaaaatt cctctatttg aaggaattaa aagtgatgaa cttgaagaaa tgttggaatg
     7561 tttaggtgtt gtagataaga cttacacaaa agatagtata atattctctg ttggaagtga
     7621 agttacttct attggaatta tgcttaaagg aagtgctcat ataataaagg aagatatcga
     7681 agggaatagg aatattgtag ctgaactctt gcctggagat ttatttggag aggtattcgc
     7741 atgtactaga ctacataaaa gtaatgttac agtaaccaca acatcttctt gcgaagtttt
     7801 attcataaag tttaaaagtg taactggtat ttgttcatct gcatgtgtat ttcataatag
     7861 gcttgttgaa aatatgctac aattaatagc tgaaaaaaac attttacttc acaataaaat
     7921 agaattactt tctcgtaaaa caactcgcga gaaattgctt atgtattttt caaaacagat
     7981 agaacaaaca ggtagccacc agtttacaat tcccttttca agaaatgaaa tggctgattt
     8041 tttatgtgtt gacagaagtt ctatgtcaag agagcttggc aagatgcgag atgaaggctt
     8101 gttatctttt aacaaaaata aatttgaaat cttttatttt aaaaaataaa atagatattt
     8161 aaacatattt atttcaatat atactatatt agttgattca aattataaat atcatatttg
     8221 aatcttaaat ttatattttt acttaaaaag ttaataggta tctaatttat taaaattaag
     8281 atacctatta actttattaa ttaagtgtac aattagctta ttttctttgt agtaattgct
     8341 gttttatcta taagccaatc ttttccttct actaatcttg aaactaacat tgatgatact
     8401 gtatttccag ttgtatttaa tatagtatta ggaatatcag ttatagtagt tataactgtt
     8461 aacagtggaa ttgcctccac tggaaaaccg aagatgctaa ccacgagcat ctcaccaata
     8521 agtcagccac ctgctacagg agccataaat actccaacaa caaatacagt tataaataat
     8581 tgacctatcg ttgctggatt tgccattcca ctacctaaaa tacctgataa aaatgccact
     8641 tttattatta ctgctaaaac tgacccatct ttatgtatat ttattccaag aggtataact
     8701 gtttctgata tatcatcagg aactcctata tttttactag cttctaaatt tgatggtata
     8761 catgctgaac ttgaagaagt agctagtgcc gttgctattg gttttatagc atttctccag
     8821 aattgtctaa acccttgttt tcctgcagat aaaaaagcat acagactgta aaataataag
     8881 aaatacatgg ctgctgttag tgaataaatt acaaacaccc ttacatatcc ttgaattatt
     8941 ttacttccaa gttccccaat tattgtagct gtataacagc ctagccctat aggagcataa
     9001 agcatgaata tacttactac ttttaatatt gcttccgttc cttctgataa aagctttctt
     9061 attgtatttc ccctatctcc catcatagct attgctgttc caattaaaac tgcaaatata
     9121 actaattgta gtaaattact actggaaaat aaatctccaa aatttttaac tgtaatactt
     9181 gatactactt ggtctaataa cgttcctttt gattctaaag tttctgcatc ttgagtcacc
     9241 attaaatttt ttacacttga tacatctaat cctttagacg gattaaataa taatgcagat
     9301 gtaataccta aaaatgcaat tacaagtgtt gttcccataa atactatagc tgtacccttc
     9361 attatttttc caaatcgttc catatttttt atactagcaa cacctgaaga aacactaaga
     9421 aaaactaatg gtactactat cattaataga gcatttatga ataaatctcc aaatggtttt
     9481 agtattgaag cttttggacc aagtactaat cctactatcg cccctactat agtacatgct
     9541 aaaagtataa ttgatgactt ataatttttt aaaaagtccc tcatttatat tttcctttct
     9601 aatgctttat attataaatt accccaaatg ctaaaaaaat taaagtaaat tatctaatgc
     9661 gcattaataa tttttgtaat tttccccaac agctacttta gcaaatactg gaatatatgt
     9721 caatttctat agtttttttg tatttttaac
//
LOCUS       DAEMGG010000141         9567 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEMGG010000141 DAEMGG010000000
VERSION     DAEMGG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.142"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            59..793
                     /locus_tag="KPK24_003244"
     CDS             59..793
                     /locus_tag="KPK24_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300891.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            1004..2185
                     /locus_tag="KPK24_003245"
     CDS             1004..2185
                     /locus_tag="KPK24_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG2300892.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKFSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            2169..2741
                     /gene="mobB"
                     /locus_tag="KPK24_003246"
     CDS             2169..2741
                     /gene="mobB"
                     /locus_tag="KPK24_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG2300893.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            2860..3135
                     /locus_tag="KPK24_003247"
     CDS             2860..3135
                     /locus_tag="KPK24_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2300894.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            3390..5090
                     /locus_tag="KPK24_003248"
     CDS             3390..5090
                     /locus_tag="KPK24_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2300895.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            5453..6640
                     /locus_tag="KPK24_003249"
     CDS             5453..6640
                     /locus_tag="KPK24_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300896.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            6700..7863
                     /locus_tag="KPK24_003250"
     CDS             6700..7863
                     /locus_tag="KPK24_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2300897.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            8014..8817
                     /gene="zupT"
                     /locus_tag="KPK24_003251"
     CDS             8014..8817
                     /gene="zupT"
                     /locus_tag="KPK24_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG2300898.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            8913..9473
                     /locus_tag="KPK24_003252"
     CDS             8913..9473
                     /locus_tag="KPK24_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG2300899.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
BASE COUNT         3716 a          955 c         1759 g         3137 t
ORIGIN      
        1 taactgtaaa ttgagatata gataaatact ataaaattta agaaaggagg attgatttat
       61 gaagttaaaa aggtcattag tttgtgtaac tattttaggt attatacttg ttggatgtca
      121 taaagaaaat actaaagaga aaaatcaagt agcaagtaaa gcaacacagc aaaaaactat
      181 gacaaaagtt caaaatgatg ttaatgaaat tatgaataaa gattataagt acattataaa
      241 gaatatgggt ataccttata atacatttta ttatataaaa cctaaagttt taaaagaatc
      301 aaatacaatg caagatataa atacatcaag ttatatgaca ttagtttatc caaaatatac
      361 tggaaatgat gaactagatg gaagtgcttt atatgttgat attaacgaaa ataaagttgt
      421 caatgttgaa accaattctt tctcatcaca aggtatatca gtcattgatg ctgaatctag
      481 tatagtgatt gaaaaaagtg accatgaaaa atcggctgta tcactagaaa attttagaca
      541 tattgacctg ggagagtatg ttggtgttga agattccaga ataaatgaaa ttgtaggaga
      601 tgcgaattat gatttaacag catataatca tgaaggcagt aaagtagtta agagttatag
      661 attgaaagaa gataacaaaa tattaaaaaa agaggtactt acaataagta ttgtggataa
      721 caaaataaaa tctataaaaa ctattgaaag tgataaaatt gtaaaaataa taaaaggaac
      781 tttattagaa taaaatattc taaagcatat gatgctgttt caagatagaa ggaaatttct
      841 agctgggaac agttttttta ttagatttat aaaatttgaa ctgcacttat agataactat
      901 tagttataat taaatataag ataaaagtat ttccaacttt tataatctat aatatattac
      961 atagaaatat cttaaataaa aaatatattg gggagtattt aaaatgatag aattagaaga
     1021 tgccataaaa attattgaaa aaaatgtttt accaataaac agggtcaaga tagtaaatct
     1081 tatagatgca gtaaataagg tagttgcaga agatatatat tcaacaattg atagtcctcc
     1141 atttgatagg tcaccctatg atggttatgc atataatgca aattctgaaa atagaaattt
     1201 aaaggttata gggactttgt atgcaggaga tgtatttaat agggtattaa ataaaaatga
     1261 agcagtgaag ataatgactg gtggaaagat accacagggt gcaaattgtg taataaaaaa
     1321 agaagatgta acagttatag gtgatatgat aactttaaat ataaaattaa aggaatacga
     1381 taattacata tttaagggtg aggatataaa aaaggggcaa ttgattatta ataaaaatca
     1441 gaatatagga tatgatggta ttggagtatt agcaagtctt ggaatatcca aagtaactgt
     1501 ttatgatgat ttaaaagttg gaattttgaa tacaggtaca gaactacaag atataaatga
     1561 gactttagag aatggtaaaa tatacgatag taatagatat accttatatt caagattatt
     1621 aaaacttgga atacaagcag atacaatatg caattgttct gatgatatat tggagttaga
     1681 aaaaaaggta aagaatatgt tacagagtgt agatttttta attaccacag gtggtgtttc
     1741 tgttggagat aaagatttaa ttccagatgt attcaaaaaa ataggggcaa cagaattatt
     1801 ttggaagata aacattaaac ctggtggagc ttgttatgta ggtacattgg gagaaaagct
     1861 attatttggt ctttctggta atccacatgc agcaatagtg gtatttgata atgtgatagt
     1921 tcctgttatt gagtatttaa catataagaa gggaacacat catataaaag ctatatttaa
     1981 aggagaattt aataaatttt caaataaaga tagatttgta agtggaaaac tatatacaag
     2041 tgaaggtaaa ttgtatgtag agctaaatga taagaaagat gcaaaagcta gactatctgc
     2101 aactctaaga agtaattgta tgattaagat taaaaagaat gagacaataa aagaaaatca
     2161 attagtggat gttgttatta gatagtttta tgaaaggaca caattttatg tataatatat
     2221 tgtcagttgt ctcatattca ggagtaggta aaactacgtt gattgaaggc ataataaaag
     2281 aattaaataa aaaagggtat cgtgttggag ttattaaaca tacctgtcat gattttgata
     2341 tggatgagga aggaaaagat acttataaac atagaaaatc tggggcgaga aaagtctgta
     2401 taatatcaga taaacgagta gcatatattg aagaactaaa agagaagaat ccattatata
     2461 aaatgattca attatatgaa gatatggact tgattatcat agaaggatat aaaaattata
     2521 gatttaaaag actggaagta actagaaaag gtaagtatga ggatatttta tctaataagt
     2581 ctgatttgat aggtattgtt agtgatatag aatataaatt gaatattgaa cagtttaatt
     2641 taaatgatta taaaaatata tcaaagtaca tagagtcttg tataaaaaat gaaattttaa
     2701 gtataagcaa tgttgaaata aaaaacatat taaaagaata attttaagat gttcttctta
     2761 tataaaatga tataattatt tatataaata aagatataag aaatttatca attttatttt
     2821 gagttataaa atattaaaga aattgtagga ggacaatata tgaaaaaaat attagtagcc
     2881 tgtggagcag gaatcgcaac atcaacaata gtttgtgaca gagtagaaag attggttaaa
     2941 gaaaataata tacaagcaga agttgagcaa tgtaaaattg ctgaatgttc aacaaaacaa
     3001 gaaggtgctg atttaatcgt atctactact atactaccaa caacttatga tatacctaca
     3061 ataaaggcaa caggatatat aacaggtata aatacaacag cattagataa aaagatatta
     3121 aatgcattga agtagttttg tatttataaa aaaaatttag tattaaatat agagttgtat
     3181 ctttataaaa ttttaaatat agagatatag ctctttttat gttgtttcaa gtatacaact
     3241 atatgatata aatcttcaat tttatgaaaa agattgttat tactttgtaa gtttataaaa
     3301 gatttaaagt tattgttatg ttatactaat ttatgggtat tttttagaag tactaataga
     3361 agtactaata aaggagggat ttaatactta tgataaaaca atttgtcaaa tattataagc
     3421 catataagaa aatttttaca ctagacctta tagcagcttt tctattttcc ttatgtgatt
     3481 tagtttatcc tatgataact agaaatataa tggatgacgt tgttccaaat aaaaacttaa
     3541 gaatgttagt agtttttgca gtagcactta tacttatatt tatagcaaaa gcagggctta
     3601 actattttat gcagtattgg ggacatgtta ttggtgtaga tatgcaagct gatatgagaa
     3661 atgaggtatt tacacactta caaagacttc caaatacgta ttttgataat aacaaatctg
     3721 gagtaactat gtctagaata gtaaatgatt taatggatat aacagaactt gcacatcatg
     3781 ggcctgaaga tttattcatt tctatagtaa tgttggttgg ttcattcttt atacttatag
     3841 atataaatat tcctcttacg cttataatat ttgctatatt accgtttata atttggtttg
     3901 caatagctaa aaaagataag atgaatattg cttttatgaa gagtagagtt actataggtg
     3961 atgtaaatgc tactttagaa aatagtatag caggtatgaa ggtaactaag tctttttgta
     4021 ctgagaaaga agagttaaat aagtttgtaa gaagcaataa attatttaga agagcaagac
     4081 aagattctta taaggtaatg gcagaatatt actctggtat gaatttatac atggatatct
     4141 tagagtgggt agttgtaata gctggtggtt attttactta tataggaaag attactttgg
     4201 gtgattttgc tgcttatata ctatatgtaa aaatgtttat acaacctatg aaaaaattaa
     4261 taaactttac agagcaatat caaaatggta tgacaggttt taaaagattc atagagataa
     4321 tggaacaaga ccatcaaaaa gaagctaaaa atcctattga acttgaaaat gtaaaaggtg
     4381 acattgagat agaaaatata tctttcacat atgaagataa gactcaagtt ttggataatt
     4441 taagtttatc tataaaagct ggtaagacta tagcattagt tggaccttct ggaggaggaa
     4501 agactacatt atgtaattta ttgccaagat tttatgaatt tgataagggt gatataaaaa
     4561 ttgatggaaa gagtataaaa gatgttagtt taaaatcatt gagaaaaaat ataggtatag
     4621 ttcaacaaga tgtattttta tttacaggaa ctataagaga taatattctt tgtgggaacc
     4681 ctaatgccac agatgaagaa atgatagctg ctgctaaaaa agctagaata catgactttg
     4741 tagagacttt gccagatgga tatgatactt atataggaga aagaggcgta aaattatcag
     4801 gtgggcaaaa acaaagaatt tcaatttcaa gaatcttttt aaagaatcct ccaataataa
     4861 tattggatga ggctacatct gctttagaca atgtaacaga aagagaaatt caagagtctt
     4921 tagaggaatt aagtaaagat agaacaaatt tagttgttgc acatagattg actactataa
     4981 aaaatgctga tgaaatcata gtattgacgg ataaaggaat tgaagaaaga ggtactcatg
     5041 aagagctagt aaacaaaaat ggagtatata gtaggttaca caataactag tatacaaatt
     5101 ttacaccata gctaacatac acattaaact tacaaacata ggatatatta agattcataa
     5161 agacaattca ttttatattt ataagatgtt ttatgaataa aatgtatcct atgttttatt
     5221 ttttgcaaat tacatttaat tctctttgta agaatagagt taaatagcca atggtcggga
     5281 taacgttttt acaaaaagaa taatttagaa aactaacttt aatttatgga atttatcgaa
     5341 ttttatgaaa aaaatcgaat tttgacttga aatgtttttc aaaagtagta aaataatgga
     5401 taactataca tacattttat aaaaatatat ttattatgag gggggatttt gtatggaggc
     5461 aataacagct ttttgtctag ttttagtatc actgacgata ggagatatag tttcagcaaa
     5521 aactaaagct tttgttccgt cggtttttgt atcagcaatt atatttatag taggattttg
     5581 gacttttttc ccagaaaata ttgtggactt agccgcttta ggaacaccat tagctcaatt
     5641 aggtatgctt ttgttaataa ctcatatggg aactatgatg agtattaagg aattagcagg
     5701 tcaatggaaa acaatagtaa tagccttggc aggaatagta ggaatatgtg taggtgcttt
     5761 ggcattaggg acagtagtat ttggatggga tacagcagta atagctacac ctcctcttac
     5821 tggagggcta gttgcatcta taatgatggc agatgctgct acagcaaaag gtcttgcaag
     5881 tttatcagta cttgcaattt tgatgtatgt tgcacaagga tttgcgggtt atcctattac
     5941 agcattaatg cttaaaaaag aaggaaaaag acttttatca gactttagaa gtggaaaagt
     6001 aactgtaaat acagatgaag aaaaagttaa ggatttacct gaacaaaaaa gcagatttaa
     6061 gataatacca gacttaccag aaaaatatga tacgacatat atgataatac ttagattagg
     6121 atttgtagca tggttagcag tacaatttac aaatcttaca aatgaagttg tatcaagata
     6181 tgttatatgt ttaatatttg gtgtaatagc atcagaactt ggaatagttg atagaaaacc
     6241 acttaataaa tctggttcat ttggatggtt aatgacatca ttgatggctt ttatatttgc
     6301 agggttagca aaagctacac cagcaatgct tgttgaaatg gcagttcctc ttgtaggaat
     6361 cataatattt ggtgtaatag gtatgggaat attctctatt atagtaggta agcttttagg
     6421 tgaaagtaaa gaaatggctt tttcagtttc gcttacatca ctttatggtt ttccaccaaa
     6481 ctatatatta actgaagagg cagtaaaggc tttggctgaa acaccagaag aaaaagaatt
     6541 tttaatggat aagatgcttc caaagatgct tgttggagga tttactacag ttacaatagc
     6601 ttctgtagta atagcaggta tctttgtaaa tctaatatag tatttaagtg ataaaaagta
     6661 ttttaaataa gaagaatgtg tactacttgg aggtatggaa tggatattaa agaaataaca
     6721 aagagttata aagactatgt aattaagctt agaagggaat ttcatgaaaa tcctgagaaa
     6781 agtatggaag aagtgagaac atcaaagaga gttaaagaag aacttgataa aatagggata
     6841 ccatatgtat cagctggagg tacaggtgta attgcaacta taaaaggagc taatccagga
     6901 aagactgtag cacttagggg tgatatggat gctctacaag ttgttgaatg cactgatgta
     6961 gaatacaaat ctaaaaatga aggacttatg catgcttgtg gtcatgatgg tcacacatca
     7021 atgcttctag gggcagcaaa agtgttaaat gatattaagg atagtatcaa tggaactgta
     7081 aaattatttt ttcagccagg agaagaagtt ggtaaaggtg caagagctat gattcaagat
     7141 ggtgctatgg aaggtgtaga tagtgtgttt ggaatacatc tttggactga tgtagaatct
     7201 gggaccatat cggtagaaga aggaccaaga atggcttctg ctgatttctt taaaattact
     7261 gtaaaaggta gaggtggtca tggttcactt cctcatcaag gtgttgatgc agtgcttgca
     7321 agttcagcaa tagttatgaa tctacaatct atggttagta gagaagtaag tcctctggaa
     7381 ccattagttg tgagtgttgg agttttaaat tctggaacta gatttaacgt aattgcgagt
     7441 gaagctgtct tagaaggtac aataaggcta tttaatccag agcttagaaa gcaaattcct
     7501 ggaattttag aacgtatagc aaaatctaca gcagaagcat atagagcaga tgctgaatta
     7561 gagtatgggt acttaacacc agcagttata aatgacaaag aatgttcaaa gatagctaca
     7621 gatgcagcaa ttaagctatt tggagaagac tgtataacac tatttgaaaa agtaacagga
     7681 gcagaagatt tagctgagtt tatgaatata gcacctggtg cactagcatt tgtaggagct
     7741 agaaatgagt caaaaggtgc ttgttatcct catcatcatg gatgctttaa tatagatgaa
     7801 gatgctctag aaattggaac tgctttatat gtgcaatatg cagtggattt cttaaataaa
     7861 taattttaat atactataaa gaaaaagcag agactaagtt tatatctctg cttttttatt
     7921 gtgtatattt tttataaata ttgactatat aaaatatata tgatagcata aagaaaatga
     7981 taatcaatat caattaaaaa gaaggtgatt tatttgaata atgtaatatt tgcatttttg
     8041 ataacattat tggctggttt atctactggt ataggaagtt gtatagcatt ttttgctaaa
     8101 aaaacaaata ctaagtttct ttctataagc ttaggatttt ctgctggtgt tatgatttat
     8161 gtttctatga tagaaatttt tcctaaagca caagatgctt tgacaaaatc tatgggagag
     8221 aaattaggaa gttggtcaac tgtaatagct ttttttatag gtatggctat cattgctgtt
     8281 attgataagc taattccaca agaagaaaat ccacatgaaa taaagaaaat ggagaatata
     8341 gaagaaaaga atatcaaaaa aaataagtct cttttgagaa caggtatatt tacagcaatg
     8401 gctatagcaa ttcacaattt tcctgaagga ttagctacat ttatatcagc tcttgatgat
     8461 gttacaatag ctattccgat agcaattgca attgcaatac acaatatacc agaaggtata
     8521 tctgtatctg taccagttta ttatgccaca ggagataaaa agaaagcttt ttattattcc
     8581 tttttatctg gtatgtctga gcctttagga gcgattattg gatatgtact tcttagaaat
     8641 tttttaaatg atataactct tggaattgta tttgcaattg taggaggaat tatggtattc
     8701 atatcactag atgaattatt gccatcagct agagagtacg gggaacatca tttatctatt
     8761 tatggattga ttgcaggaat gggtgttatg gctattagtt tattactatt taaatagctt
     8821 tttaaatata tgattgtaac ctaaaattag tatcaaaatg atataattat ttaaatagat
     8881 ttttaagcta tttaaaaata tcattttgga gaatgagtgc tatggaaaag aaaataaaat
     8941 tcaaatttga ctggtatagt tcacctgata agatagagaa atggttagaa gatattgaag
     9001 ctaaaggata taatttatat aagataaata aatttggagg aatattttat tttcataagg
     9061 gaagtccaag aaaaataaaa tacactgttg attactacta tgatgtagag aataattatt
     9121 ttgatgaaca taaatcaaat ggctggaatc ttgtatttag gagtattggt agttttatgg
     9181 gtacatctgc atggtatata tggtcgaaag aatacaaaga catacctcct aaattatata
     9241 gtgattctga taaaaaaaac aaagtaaagt caattttatt tatgaatatg tttatattct
     9301 ttatgttagt agtaatattt atatatttat tgaatattga aataagaaat ttatatagtg
     9361 gttatggagc atctgtaact gctattgtac tacagattat attgttgatt ttatatgcag
     9421 tacaattata taggagatta atgtattatt taaggcttaa aaagtttaaa taaataaaag
     9481 aaggtgtcct aaaattattt ttttaatagt ttaagacatc ttcttttatc agtaagtact
     9541 gtttttaatt atagtaattt ctgtctt
//
LOCUS       DAEMGG010000142         9552 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEMGG010000142 DAEMGG010000000
VERSION     DAEMGG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.143"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1027
                     /locus_tag="KPK24_003253"
     CDS             <1..1027
                     /locus_tag="KPK24_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG2300900.1"
                     /translation="LFVVGCSNNPNKESTIKKDSKNNQSQVKEEKREPVKKDITISFA
                     GDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDLTVVNLEGPLTTASN
                     AKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDTQFILDENKINYFGL
                     GKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKSDVVVVYFHWGIELD
                     YYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYSLGNFCFGGNKNPSD
                     TDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKILDGSEKDRVLNKLEK
                     ISKDLNK"
     gene            1197..3098
                     /gene="cooS"
                     /locus_tag="KPK24_003254"
     CDS             1197..3098
                     /gene="cooS"
                     /locus_tag="KPK24_003254"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG2300901.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAECIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3091..3564
                     /locus_tag="KPK24_003255"
     CDS             3091..3564
                     /locus_tag="KPK24_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2300902.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3539..4765
                     /locus_tag="KPK24_003256"
     CDS             3539..4765
                     /locus_tag="KPK24_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2300903.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(4904..6091)
                     /locus_tag="KPK24_003257"
     CDS             complement(4904..6091)
                     /locus_tag="KPK24_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2300904.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6364..7188
                     /locus_tag="KPK24_003258"
     CDS             6364..7188
                     /locus_tag="KPK24_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG2300905.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7317..8582
                     /gene="gluD"
                     /locus_tag="KPK24_003259"
     CDS             7317..8582
                     /gene="gluD"
                     /locus_tag="KPK24_003259"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG2300906.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8795..9385
                     /locus_tag="KPK24_003260"
     CDS             8795..9385
                     /locus_tag="KPK24_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG2300907.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
BASE COUNT         3704 a         1045 c         1743 g         3060 t
ORIGIN      
        1 gctatttgta gtaggatgtt ctaataatcc aaataaggaa agtaccatta aaaaagactc
       61 taagaataac caaagccaag ttaaagaaga aaaaagagaa cctgtgaaaa aagatataac
      121 tataagtttc gctggtgatg taactatggg gaactataaa ggttcatcat attatggcag
      181 ttttgaccat gaatttaaaa gacaaggaga agattacaat tattttctta aaaatgtaaa
      241 acctgtattt gaaaaagatg acttaacagt ggttaattta gaaggtccac ttactacagc
      301 gtcaaatgca aaagaaaaaa aatttgcatt taaaggaaag cctggatatg taaatatttt
      361 aaaatctgga agtgtagagg cagtaagtat tgcaaataat cattcagaag attattttga
      421 agaaggaatg aaagatacac agtttatatt ggacgagaat aaaataaatt attttggatt
      481 agggaaagat gctgttgtag atgtcaaagg aattaaagtg ggtctattag ggtataatgg
      541 attatcaact gaatataatg aaaaaaattt aaaacaaatg gaagatgata ttaagtcctt
      601 aaaaaagaaa tcggatgttg ttgtagtgta ctttcattgg gggatagaat tagattatta
      661 tccagataaa aaacaaaaag attttgctca ttatgctata gacaagggag cagatttagt
      721 tatggggtct cacccacatg tgattcaagg aatagaaaag tataaaaata agtatatagc
      781 ttatagtcta gggaattttt gctttggagg aaataagaat ccttctgata cagatagtta
      841 tatatatcaa caaaccttta ctttttcaga taataaatta acttcaatta aagagccaaa
      901 tataatacca acttcaataa catctagtaa ctccagaaat aattatcaac caaaaatatt
      961 ggatggaagt gagaaggata gagttttaaa taaacttgaa aaaataagca aagatttaaa
     1021 taaataaaat gtaaaaaaaa ataaatatat atatttattt taataattta tattttgttt
     1081 agggtatggt aaatcgtggt attaaataag tagattttaa tagttaaatt atagtcaatt
     1141 ataataaagc tatttaatct acttttttaa tataaaaata taagggggaa tttgtaatgg
     1201 cttgtaacac ttgtaaaatg tgcgaaagtg cagacaagaa attagaaagt tttgtagctt
     1261 caaaggatgt tgaaacagca tttcatagaa cagaagatca gaaagttaag tgtggttttg
     1321 gacttcaggg ggtttgttgt agactctgtt caaatggacc atgtagagta actcctaaat
     1381 caccaagagg tatatgtgga gcagatgcag acactatagt agcaagaaac tttttaagag
     1441 cagtagcatc aggagcagct tgttatttac atgtggtaga aaatacagct aagaatttaa
     1501 aaaatgttgg aataactaaa ggtgttgtta aaggagaaaa aacattaaat caattagcag
     1561 agatgtttgg aatagaaagt gatgaaaagt atgataaatg tataaaggtt gcagacaaag
     1621 ttatcaacga tttgtataaa tcaagagatg ataaaatgga attagtagaa aaaatagctt
     1681 atgcacctag agtgaaaaag tggaaagagt taggtataat gcctggtggt gcaaagtcag
     1741 aagtttttga tgcaatagta aaatcatcaa caaatttaaa cagtgaccca gtagacatgc
     1801 tagttaattg tttgaactta ggtatatcaa caggattata tggtcttaca ctaactaatc
     1861 ttttaaatga tgttatgttg ggagaaccag ttataagaat ggctcctgta ggatttaatg
     1921 taatagaccc agactacata aatataatga taacaggtca tcaacattca acatttgcaa
     1981 atttccaaga tagattaaaa gatgaagatg ttataaaatt agctcaatct gttggcgcaa
     2041 aaggatttaa gttagtagga tgtacatgtg ttggtcaaga tttacaatta agaggagaac
     2101 attatcaaga agtatttgct ggacatgcag gtaacaattt tacaagtgag gctgttttat
     2161 caactggagc aatagatata gttctttcag aatttaactg tactatacca gggcttgaac
     2221 caatagcaga taaatataaa gtaaaaatgg tttgtttaga tgatgttgct aaaaaagcga
     2281 atgcagaatg cataggttta gacagaagta aattagatga acttagtaac actttaatag
     2341 aaaaagcatt ggagtcttat aaggagagaa gaggaagtat tgaaatagat ataccaaaag
     2401 accatggatt tgaacaatct ttgactggtg taagtgaaaa gaacttgaaa gcgtttttag
     2461 gagattcatg gaaacctctt attaatttaa ttgcagaagg aaaaattaaa ggtgttgcag
     2521 ctgttgtagg atgttcaaat atgacagcag gaggacatga tgtaaatact gttgaactta
     2581 ctaaagaatt gattaaaaaa gatataatag tactatctgc aggatgttct actggaggat
     2641 tagaaaatgt tggtcttatg tcacctggag cagaagaatt ggcaggagaa aacttaaaag
     2701 aagtatgtaa gactttagga ataccaccag tattaaactt tggaccatgt ctagctatag
     2761 gaagactaga aatagtagca acagaattag cagctgaact tggaattgac ctaccacaat
     2821 taccattagt gctttcagct ccacaatggt tggaagaaca agcattagct gatggagctt
     2881 ttggtcttgc ccttggctta cctcttcatt tagctttacc tccatttata acaggaggaa
     2941 aattggtaac agaagtatta acagaaaaat taaaagactt aacaggagga catgttatag
     3001 tgaatccaga ccctaaatct tcagctaatc aattagaaga aataataata gatagaagaa
     3061 aaaatttagg gttaaaggat gtggaatgca atgcatagaa tttttataaa taaagattta
     3121 tgtacaggat gtaagagctg tgtactagca tgtatgctaa aacacaataa agattatgac
     3181 atgtacactc ttgatttaga aaatatagat aatgatagta gagggcatat agaattagat
     3241 agtagacata ataatccagt tccaattctt tgtagacact gtgatgaacc tgaatgtgta
     3301 ttagcatgta tgagtggtgc aatgcataaa gatagtgaaa gtggaatcgt ttcatatgat
     3361 gaagagaaat gtggttcatg ttatatgtgt gtaatgtctt gtccatatgg attattaaag
     3421 ccagatgata gaagcaagca gaacatactt aaatgtgatt tatgtaaaga tgaggagtat
     3481 cctagatgtg tagctaattg cccaagtgga gcaattgaat tacagaagga ggaaaatgat
     3541 gaattatgta gtgttagggg ctagtgcagc tggtataaat gcagtaaaaa cattaagaga
     3601 attagataaa gattcaaata tagtagtaat ttctaaagat gaaaacgtat attcaagatg
     3661 tatgttgcat cacgttatat ctgaacacag aactttaaaa caaataaatt ttgttgatga
     3721 agattttatg gagcaaaata atgtaaaatg gattgcaggt aagactgtta aaggtataga
     3781 cataaataaa aaggtagttc agacagaaga cattactgta aattatgata aacttttaat
     3841 agctacaggt gcatcttctg ctattcctcc tataaaaaat ttaagagatg gaaattttgt
     3901 atattctgtg agaaacatag atgatattta taaaataaaa gaaaaagcac aaaattctaa
     3961 aaatgtagtt ataataggtg ctggtttagt tggtatagat gcattagtgg gattatttaa
     4021 atatgagcag ttaaatattt ctgtagcttt tatggaaaag tacattttag atagacaatt
     4081 ggatgaatat acagcatcag tgtatgaaaa taagttcaaa gaaaaaggtg taaaatttta
     4141 cccaagtgca tcaatacaag aaattgtatt ggataacagt aaaaatgtta caggtgttgc
     4201 attttcaaat ggtgaagttt tagatgcaga catggtaatt gtagctacag gggttaaacc
     4261 aaatgctgac tttttagatg gaacaggaat agaatatgat agaggtataa taatagatga
     4321 tatgtgccaa acaacacaaa aagacatata tgcagcagga gatgttgtag gtaaaaatgc
     4381 tatttggcct ctggcagtaa agcaaggaat tgtagctgcc tataatatgg ttggaaaaga
     4441 taagaagata gaagatgaat ttgcttttaa aaatagtatg aatttcatgg atataccaac
     4501 tatttctata ggaatgaata cacctgtaga tgatagctat aaagtattaa ccagacatgg
     4561 aattaatgat tataaaaagt ttgtttttaa agataatgta atctatggag cagttataca
     4621 aggagatatt tcatatgtag gtgtattaac ttatttaata aaaaataaag ttgaaatata
     4681 tgatttagaa aataggatat ttgatatatg ttatgcagac ttttttaata taaaagaaaa
     4741 tggagaattt tgttatagtg tatagtatat acgtggagta aggtttataa atacagtaaa
     4801 tcaataaata gagttattct aaaataagaa aaatgacaaa tcttatttag aaatagctct
     4861 attttttgta attaaattta aattatattc aatttgtaaa atattatttt gaacaattat
     4921 acatatactc tctagttaga ggtaaatcat tttttatacc tttagtgaaa agtatttgat
     4981 gtatatctat aataccagtg ttgaaagcag cagcacaaga acaaaggtat aaatcccaca
     5041 ttcttataaa cttatcatca aacatttctt taatctttgg aagattctca gagaaatttt
     5101 tttgccagta tagtaaggtt tttacataat gtaatcttaa actttctacg tctatagtat
     5161 gatatttgta atcactacaa atatgtatca tttctcttag gctaggaata actcctcctg
     5221 ggaaaatata tttttttatc caagggtctc cataagattc ctgcaatcca cttatatagt
     5281 gaagtaaaaa caatccacca tcttttaaaa cagcatttac gtttttaatg aaaagttcat
     5341 agttttctct accaacatgt tcaagcatcc caacgcttac aactctatca aacataaggt
     5401 ctgatttttt taattctcta tagtccataa gtcttacctc aagatagtct tgaaggttct
     5461 cttctttaat tcttgcgtta aattccttaa attgctcttc gctaagtgta attccaacgc
     5521 cgtgaatttt atattttttt gcagcttcta ttagtaaaaa tccccaacca caaccaatat
     5581 cgagaaggct cataccttcc tctaaattta gtttagttaa tatgtggtga actttattga
     5641 cttgagcatc ataaagagta tcattatcat ttctaaagta tgcacaagaa taactaagtg
     5701 tcttatctaa ccataagcta taaaaatcgt tgccaatgtc atagtgggca tgtatttctt
     5761 tcttttgatg tctcattgaa tttgatgagt gcaatagatg ttttacagct gaattatcaa
     5821 tattgaattt gtctatttgt tttaaaagta gatttaaagt taagaataaa tctccctcaa
     5881 cttcaatgtc tctatttatg taggcttctc caagtgctaa agatgtactt tttaatagtt
     5941 cagacttgct aatatcatta tttactataa ctttgaactg aggggagccc tctccaatta
     6001 gaaattcttc ctcatcttta agcctaacta gaaatggtgt ttcaccaaat ttctctaaat
     6061 agtcttttaa aaaatttttt gtaatattca tattaccagc tccttctaat gtaaatccag
     6121 taagtttccc tgcttaaaat atattatagt ccttttttta aaaaagtaaa attttttcaa
     6181 taaatttttt atttaaagga aatttatttt ttgtaaagaa atttcatgtt gtagtatatt
     6241 ttttgcttat aaattttggt actggatata atattaaact gttaatgata taataaattt
     6301 aatctataaa gtaaataaaa ttttgtaata aatagttaaa gctattttat aaggagatat
     6361 atgatggaag tatatgcttt ggttgggtca agtggcacag ggaagagctt taaggcttta
     6421 gagttcgcat atgaaaatga tatagaatat ataattgatg atgggatttt aatatacaaa
     6481 aacaaggttt tagctgggat atcggcaaaa caagcaaata ctactataga agcagttaaa
     6541 agggctattt tttataatct ggagcataga caagaagtta gagaagcaat taaaaaagaa
     6601 aatataagaa aaattcttat tattgggaca tcaaaaaaaa tggttaatca aattgtagag
     6661 agattgtcaa ttggaagggt ttgcaagttt ataaatatac aagatataag tactaaatat
     6721 gaaatagaaa tagcaaaaca agctagaaaa gaaggaaatc atgtaattcc tgttccagca
     6781 gttgagataa aaagtatggc aagtggtctt agtataaatt ctttgaagag actttttaga
     6841 aaaggaaata ataggaatat ggtagtttta gaaaaaacaa ttataagacc aacttttagt
     6901 tatataggaa agttttatat tagtgctgat gtaattaaac agattattga atatgaagta
     6961 tacaactttg gaagcattga tagaataaat aaaatcaata tagagaattg caataatttt
     7021 atgaatattt ttgtaagtat aaatatcaat gatttagaaa ctataaaaaa aattgaagac
     7081 atacaacaga caataaaaaa gtctattgag aaaatgactt tagttaatgt acaaaaaata
     7141 gatattaaca ttcataaaat taaaaaaata aacttcctcc aatgttaaaa aataaactga
     7201 gaaaatgata tactaatttg caataaagtg tgctataatt aaactgtaaa tttaggaaat
     7261 ggttttttct aaatggagta tacataatta taacgtattt ataagggggc tttcaaatgt
     7321 caggaaaaga tgtaaatgtc ttcgagatgg cgcaatctca agtaaaaaat gcatgtgata
     7381 aattaggtat ggaaccagca gtttatgaat tattaaaaga acctatgaga gttatagagg
     7441 tttcaattcc agtaaaaatg gatgatggtt ctataaaaac ttttaaagga tttagatcac
     7501 aacataatga tgcagtaggg ccaacaaaag gtggaataag attccatcaa aatgtttcaa
     7561 gagacgaagt aaaagcttta tctatatgga tgactttcaa gtgttctgta acaggtatac
     7621 catatggtgg aggtaaaggt ggaataatag tagatccatc aactttatct caaggtgaat
     7681 tagaaagatt aagtagagga tacatagatg gaatatataa attaataggt gagaaagttg
     7741 acgttcctgc accagacgta aatactaatg gtcaaataat gtcttggatg gttgatgagt
     7801 acaataaatt aactggacaa agttctatag gtgttataac tggtaagcca gttgaatttg
     7861 gtggttcttt aggaagaaca gctgctactg gatttggtgt tgctgttact gcaagagaag
     7921 ctgctgctaa attaggaata gatatgaaaa aagctaaaat agctgttcaa ggtataggaa
     7981 acgttggttc ttatacagtt cttaactgtg aaaaacttgg tggtactgtt gtagctatgg
     8041 ctgaatggtg taaatcagaa ggttcttatg ctatatacaa tgaaaatggt ttagatggtc
     8101 aagctatgtt agattatatg aaagaacatg gtaacttatt aaacttccca ggagctaaga
     8161 gaatatcttt agaagagttc tgggcttcag atgttgatat agtaatacca gctgcattag
     8221 aaaactctat aactaaagaa gttgctgaat ctataaaagc taaattagtt tgtgaggctg
     8281 ctaatggacc aactactcca gaggctgatg aagtatttgc tgaaagagga atagttctta
     8341 ctccagatat attaactaat gctggtggag ttacagtttc ttacttcgag tgggtacaaa
     8401 acttatatgg atactactgg tcagaagaag aagtagagca aaaagaagaa atagctatgg
     8461 ttaaagcatt tgaatctatt tggaaaatca aagaagagta caatgttaca atgagagaag
     8521 ctgcttacat gcattcaatt aagaaagttg ctgaagctat gaaattaaga ggatggtact
     8581 aagatttact cttaataaat aataggatat aattatttaa gatataaagg ggctgtacat
     8641 agtacagccc ttaaattatg tggcttacaa tgaagtaagc tatttttgtt ttaacttata
     8701 aataacttga taaattatgt aatttagaat gtatttttta ccaccacatg attacaaatt
     8761 agaggagtta gattgaattt ttagacatga acttatgcat tttaaaagca aagaccttaa
     8821 tttaaagtat ctagtattat ttttaaaaat agtatattgg tttaatccat ttatatgtat
     8881 aatggaaaaa tatatggatt tggattgtga actttattgt gatgagagag ttttaaaaaa
     8941 ttgtactgta caacaaaagc aagaatatgc tctaactatt gtaaattcaa tgaaaagaag
     9001 tacaaatttt tcaaataaat ttgtagctgg attaaataaa caatctgata ttagaaaaag
     9061 agtaataaac atgttcagtg aaaaacataa aaatggtatc ttaagggctt tgattataag
     9121 cttgttttct tctattacat tttttaaatt cgattcaact agtattattg aagagaatga
     9181 tttcttagag aggtcaagat atacgtttga aggaaggcaa gaagacatgt ttgtaacaat
     9241 agattttata tatgcagatg tacctataaa acttagaaaa gagcatgaag aaaactgtag
     9301 agtcaatgga atagtaccaa aagattcaga ttcaatagaa attattccta gattttataa
     9361 agattgtatg gatttaaaaa aataagttag acttaatatt gatgttgata ttatgaattt
     9421 tttatattta agcaattgaa aattttatta tagattcaat tttatatgtg tttaagtatg
     9481 aaatttatag atagttgaaa taattaaata tgattgatgc ataggaatca ttactaagct
     9541 ataattaatt at
//
LOCUS       DAEMGG010000143         9198 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEMGG010000143 DAEMGG010000000
VERSION     DAEMGG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.144"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..758
                     /locus_tag="KPK24_003261"
     CDS             <1..758
                     /locus_tag="KPK24_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG2300908.1"
                     /translation="LEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFNIIDTFNS
                     YVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCVWGAGDLK
                     ELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELDEKMSYHN
                     ALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDEFRKILNR
                     ELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(850..1602)
                     /gene="xth"
                     /locus_tag="KPK24_003262"
     CDS             complement(850..1602)
                     /gene="xth"
                     /locus_tag="KPK24_003262"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG2300909.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1763..2713)
                     /gene="galT"
                     /locus_tag="KPK24_003263"
     CDS             complement(1763..2713)
                     /gene="galT"
                     /locus_tag="KPK24_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG2300910.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3098..4294
                     /locus_tag="KPK24_003264"
     CDS             3098..4294
                     /locus_tag="KPK24_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2300911.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4406..5746)
                     /locus_tag="KPK24_003265"
     CDS             complement(4406..5746)
                     /locus_tag="KPK24_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2300912.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     HQIAIVITENNGAISNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(5885..6637)
                     /locus_tag="KPK24_003266"
     CDS             complement(5885..6637)
                     /locus_tag="KPK24_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2300913.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(6785..8362)
                     /locus_tag="KPK24_003267"
     CDS             complement(6785..8362)
                     /locus_tag="KPK24_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2300914.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     MPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGIIAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYAIVYYNVFKILILRFNLQTPGREEITDINFDLKPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(8774..>9198)
                     /locus_tag="KPK24_003268"
     CDS             complement(8774..>9198)
                     /locus_tag="KPK24_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2300915.1"
                     /translation="FLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRALVFIKNYGVI
                     SMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEEGDKIATDFMD
                     KLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT         3222 a         1475 c         1073 g         3428 t
ORIGIN      
        1 atttggaatt taaccaagga tttgataaaa aactcaataa aactgtatcc aatgaaaagt
       61 gtccttttga aattatacaa ataggagcaa ttaaattgga ttctaaattc aatataatag
      121 atacgtttaa tagttatgta aaacctacaa tttataaaga catacatccc tttattagtc
      181 gtatgactaa tattaaaaat tctgatttta atgattcgcc tactttccct gaggtctaca
      241 acaattttat aaaatttata tcatcacaag accctatatt atgtgtttgg ggagctggag
      301 atttaaagga attatacagg aatatcaact atcacaagct accatcaaat tccttaccta
      361 aatcttatat taatatacaa caacatgcat ctaaatattt caataatcca gcaggaaaaa
      421 gcattggcct acaaaatgct atttctatat tagaacttga tgaaaaaatg tcttatcaca
      481 atgctttaaa tgatgcatat tatactgcta aagtttttat taagatatac aatccaagta
      541 tagtcccaga tatatatctt tatacaagta taaaacctaa aactataaga tattctaaca
      601 aaaaaagagt tgattatgat aagttatttg atgaatttag gaaaatttta aatcgtgaat
      661 taactaaaga cgagaaaaaa atcattaact tagcttataa tatggggaaa acaaatcaat
      721 ttacattaga aaatgttaag caaagaaaaa acaaatagag tttatttaga taaaaaatag
      781 gcatgtagat taaaaaacta gttaacttta aatttatatc tacagcccaa tttttatcat
      841 aattaatttt tattttatct ctaattcaac tggacaatgg tctgaaccta atatttcagt
      901 gtgtatatcc gcacttacta atctatcttc taattttttg gatgcacaaa aataatctat
      961 tctccaccct gcattatttt ttcttgcatt aaatctatat gaccaccaac tataaacacc
     1021 ttctttatca ggattaaaat atctatatgt gtctataaac ccactgtcta ataatttaga
     1081 aaacttctct ctttcttctt tagtaaaacc tgcatttttc atattatttt taggattttt
     1141 taaatctatt tctttatgtg caacgttcat atctccacac acaattacag gcttatgatt
     1201 atctagtttg gttaaataat ctataaagtc gtcttcccat ctagttctat attctagtct
     1261 ctttaattca gattgagaat ttggcgtata tactgtaaca aagtaaaaat cttcaaactc
     1321 tagagttatc actctaccct ctttgtcatg ttcttctata tttattccat acattacttt
     1381 taaaggctct ttttttgtaa atatagcagt acctgaataa ccctttctct cagcataatt
     1441 ccaatattga aaatatcctg gtaaatctaa ctctatttgc ccttcttgca atttagtttc
     1501 ttgtaaacaa aatatatctg catctacttc tttgaaaaag tctaaaaatc ctttcccaac
     1561 acaagctctt attccattaa cattccatga tataaatttc atacttatac tcctcatcta
     1621 aacattgaca cactccccat agctaaagat gggggctttt caacaatttt attgtaaatt
     1681 cttaattcac ttttatttta acaatgctat actataattt catctaaaat tactatttac
     1741 ttaataccta aaaatatatg ttttaatcaa tttttaactt ttttgttaaa tcttcagggc
     1801 tcattgaaga aacatacata tttgttccaa gttcaaatcc tccaaaatta tattttcttg
     1861 ggattatgtg catatgaaca ttaaaatacg attttgtttc aatctttgtt ggatgagtgt
     1921 gaatacataa attaaatggc atatatccat tgatattata taacttctta aacaactttt
     1981 caaatattgt agaaagctcc tgtatattat tatcatttaa ctcaccaaac tctattttat
     2041 ctttaaatat tattcttact tctccactat acttggttgc atatggtata agtaccaaaa
     2101 aagcttctcc attataaaca actcttctat ctaaacttat ttcattttct attaaatcat
     2161 catacaaaga gcttttattg ctgtcataat actcctttga aaccaacagt tcattcgtaa
     2221 tttcaggtgg aataaaagac atagacaata tttgagcatg tggatgcatc aaagatgctc
     2281 ctgcctttct caagaagttt ttgaagatac taacatactc tactttatca tctttcgaca
     2341 aatctctaaa tctatttcta tacataaaaa aaacatcttc aaattctttt tgactcatgt
     2401 tataaaaact acgattatgt ttatcgctct ctatcattac ttcatgaaca ccatatacat
     2461 ctcttgccat atcatcaatt ataggaaatt tattgtagac agattttaca agccatcctt
     2521 cttcactttc tatttcaaat aatgcatcag ttgcatgagc ttcatttcct ttgcaaaatg
     2581 ggcattcttc atcatattca ttagtttctt catcttcatt tgggatatct gctgtatcta
     2641 aaggtctttt taacctatct gtagcaaata taacaacatc attggtaatt ggatctattc
     2701 ttaattcttt cataattaac acctcttagt taatagcctt taatataatt atatgatatt
     2761 tatcgtatct gttaaataaa tttataatat tttttataat tttgcatatt tattattata
     2821 tattttgtct atagctgaaa gattataact aaaaaatcac tatcagtata tcagaatatt
     2881 atcttactat aaattaattt atgaatacat aatttagtgg cataaacatg ggtattttac
     2941 tataatttgt taaaagttta ttttaaagct atattaattt tttttgatga tttttaccca
     3001 taagtatgtt attattatct tgtaaagatg tcttgtattc aaacatacaa tttacataat
     3061 acaatatacg tatttaaatt aaataggggg tattaaaatg aattattcaa acagagttag
     3121 tgcaatgcaa gcctctccaa tcagaaaact tgttccattt gcacaagcag caaaagataa
     3181 aggaatcaaa gtatatcact taaacatagg acaaccagac ataaaaactc ctaaaggatt
     3241 ttttgatgct gttaaaaact ttgatagcga agtcttagaa tatgctacat cagaaggtat
     3301 tccagaatta ttagaagcac ttcaaaatta ctataaaact tacgatatga attttgaaaa
     3361 agatgaatta ttagtaacta atggtggtag tgaagcttta cttttcacta tgatggcagt
     3421 ttgtgaccca ggagataatc tattagttcc agaaccattc tatacaaact acaatggatt
     3481 tggtcaatct gtaaacgtag aagttaatgc tgttacaaca aaggcagaaa acggtttcca
     3541 ccttccatct aaagaagaaa tattgtctaa agtagatgat aaaactaagg ctataattct
     3601 ttctaaccca ggaaatccaa ctggtgcaat ttatacaaaa gaagaactta atatcctagc
     3661 tgaaatagct aaagaaaaag atttatggat aatagcagat gaagtttata gagaatttgt
     3721 ttatgatggg cttgagtata caagctgtgg taacttagag ggtgtacagg atagagttat
     3781 aataatagac agtgtttcta agagatacag tgcttgtggt gctagaattg gttctatcgc
     3841 ttgcaaaaat aaaggtctta tcgctcaaat ccttaaatta tgtcaaggaa gactttgtgt
     3901 tcctacatta gagcaaatag gagctgttga gttatacaaa actcctgttt cttacttcaa
     3961 agaagttaat gaagagtaca aaaaaagaag agatgtatta tacaatgaat taatgaaggt
     4021 tgaaggtgtt atatgtaaaa aaccaactgg tgctttctat atagttgcta aattaccagt
     4081 tgaaaatgca gaagatttca caatttggat gttaaaagaa tttaacaaag ataatgaaac
     4141 agttatggtt tgccctgctg aaggattcta tgctactcca ggtttaggac gtgatgaaat
     4201 aagattagct tacatcttaa atgaaaaaga ccttcataga gctgctacat tattaaaaga
     4261 aggtttagag caatatgtag ctttacaaaa ataatttttt aaacttaatt tatataaatt
     4321 taatgatata aaatgtcccc ttttcagata ttatttgaaa aggggacatt tcttaattaa
     4381 tatattaatt aagacttaga cctgactatt tttcattttc tttatatagt gttggccagt
     4441 aatctttatt cactttaatt aaagcatcta aaacttgtct tgccttagtt ggagagatta
     4501 tagttctatt taatgttaaa gataaaatag ctttttcata agattgttca aagtatgcat
     4561 caactgctaa cttttcataa gctagttgac cttctaacag tcccttataa aatgttggta
     4621 tattacctac atgtaaaggt ctcggtccat tctttgtaag aatcgcagct acttctacca
     4681 ttgcatcatc aggtaagttg ctaatagccc cattattttc tgttataact atggctattt
     4741 ggtgtttgtt atgagctatt gcttctgcaa cttcaactat aaattcccca tgagcatcat
     4801 tatgcactaa attagaacct atagatgttc ctttttctat aacagattta cattcttcaa
     4861 atacacgctt ttctcttcca ttcataactt ctcttgctct agtacaattt atatttgatt
     4921 tagctacttt tttatcagga tatagataat actgtacata tgtattaggt aagtaatctg
     4981 gtacatcttc caacatatct ttaaccattc cataagtgtc taaccaagat ttatctcttt
     5041 gttctgcatc aacaggttgg aatccagagt ttgctactaa atcttttatc ttaggtacca
     5101 aatcttcacc atttttatca tacaaatgtg taaaccatcc aaagtgattt aatccaaagt
     5161 atacaggctc aaatgttctt gaatccatgt ctaacaatct tccataagac cttaacaagt
     5221 taactggttg gtcacacata ttaagtaatc ttttatcatc tggaaatact ctttttaatg
     5281 cttctgcaac aatagcagca ggatttgtat aatttaatat ccatgcttta ggagagtatt
     5341 ttcttacagt cttaaccatt tctatcatat cacctataga ccttaaccca taagcaaatc
     5401 caccagctcc acaagtttct tgaccaacca gtccaaattg tagaggaatc ttttcatcta
     5461 attctcgcat cttaagacca ccagttctca tttgaacaaa tacaaaatct acatctataa
     5521 atgcttcatc tgggtttgta gtatattcaa gtgttgcttt aggatattct tctttaaata
     5581 atatttttcc aaattcccct attaatcttt gacgttcttc tactacatct aacatagtta
     5641 ctttatttag aggaaatcta tcactcattt tacataaaga ctttaaaagt cctggagtcc
     5701 aagtactgcc tcctccaact atagctatat tgtatctttt tttcattgtc atattaatca
     5761 cccctgtttt ttattaacta acttgtaaga tgattataat atataaagtt ttctacatga
     5821 atacttttag gtaatcatta aaaatagttc aattttagta tttttttcaa aattgtaatt
     5881 tttttcatct attgtatttt tcaatgtatt tatgaaacat atattccata agagaaagtg
     5941 ctggcactct agatgtatag ttaacactat taaaattata ttgtggagaa gatacaaata
     6001 attttatatc agctatattt gataatgaat tgacacttat atttgtcaga gaaattactt
     6061 taacttgatt tgtttttgct atattacatg ccttaataac ttgagcagtc tctccagaca
     6121 tagatacagc aattacaagg tcatctttag taatatttga tgcagccaaa tacatgagag
     6181 ttgaatcatc atagtatttt acatttacac ctatagctga aaaacgttga gacatttcag
     6241 tacatactaa tctagaagac ccaacaccaa aaaaatatat atgcttagaa ttatgtattt
     6301 catttaaaca catatctata ttctctacat ttattaaatt ttgtgtatcc ataacatcag
     6361 taaaaatatc actttgactt ttagtaaact ccttatttct ttcatttcta tttgaatatg
     6421 aaaaaactaa aaagttttta aattcactat atccagaaaa gccgagcttc tgacaaaacc
     6481 ttactatgta tgcagtcgaa ttaaaagttt catttgctat atctttaatc tttttttgat
     6541 ttattttttc agaatttttt agaaaatagt ttaaaagaga tatttcattt gtagataatt
     6601 ttttttgatt tatatctaat ttgtctatta ggttcattta tacctccata ataatgattt
     6661 gtagttaaac aactttctca tatttattat ataaatatac actatacagc atttatctta
     6721 tgtttattat actataaaaa agacaataca aattataaat tagtattgcc ttttaatatt
     6781 tactttaaat agacattgca aaagaaactt cgtctcttac ttgagataca gatggcccat
     6841 aaattatttg gatatcttta tcagatactt ttataactcc cattgcacct gtatattttt
     6901 taaaagcatt ttcatctact ttactcatat ctttaactac tactcttaag cgagttatac
     6961 agtttgttat atcttctata ttgtccttac cacctaaata tttaattata gataatgctc
     7021 tcttagtagt ttctccactt tgagctattt ctactttatt tgataaaggt tttaaatcaa
     7081 aatttatatc tgttatttct tctcgtcctg gagtttgtaa gttaaatcta agtatcaaaa
     7141 tcttaaatac attataataa actattgcat acactaaacc aagtattata gaataaatcc
     7201 actttgtatt tggttgcata actccaaata ctactaaatc tattatgcca ccttgagtat
     7261 ttcctattgc tactccaaat atatgcatca tcataaatga tatacctgat aaaaatgcat
     7321 gtataacaaa taatattggt gatacaaata agaatgaaaa ttccaatggt tccgtaattc
     7381 ctgttacaaa tgttgctaaa gcaccagcta gtaatatacc tttaaccttc gacctatttt
     7441 caggctttgc agttttatac atggcatatg cagcagcagg aagaccaaat accattatag
     7501 gtattttccc ttgagctaaa aatctagttg cttctgttga aaaactgatt gtttcagggt
     7561 cacttagttg agctaagaat atgttcaatg ctccaaatac ttcttgccct cccacaacta
     7621 aagaacctcc tacttcagta aatctaaaca tagcatttaa tatatgatgt aatccaaatg
     7681 gtattaaaag tctttcaaga aatccaaata caaatgttcc taaatatcct aaactagcta
     7741 taccaatacc tacactctga ataattgtat ttacatatgg ccatatatat ggtattatta
     7801 tccccaaaat agagaatact actgttgtta ttattggaac aaacctagtt ccaccaaaga
     7861 atcctaaata atctggcagt tttatattat agtatttatt atgtaatgat gctgttatta
     7921 taccaactac aattccacca aatactccta attctaatgt ttgtatgcct aaagttacag
     7981 cttgacccat ttctctcatc atgtcaggtt cagcaataat ccccctacac cctaacatat
     8041 aatttacact agtgtgtaat actaaataac caacaaaacc agaaaatgca gaaactgctt
     8101 tttcttcttt agcaagacca acagatattg atatagcaaa gaatattgct aagtttccaa
     8161 aaagtactcc tgttatagct tttgtaaaac ccaatattcc ttgaataaaa ttatttgcta
     8221 aaaaaggcat atcactaact atattaggat ttgaaaatgc tgaacttatt cctaaaaata
     8281 tacctgctgc agatattaat gctattggca ataaaaatga ttttcctaac tgtaaaaaga
     8341 accctttcat tttttgtttc atttctatcc ctccattttt aaatttttaa aacgttttct
     8401 atgtcataat tatatatttt tattttgttt ttgaaacttt ataatttgtt ttaaaatatt
     8461 tttacataaa tagtaatttt ttcaaaactg taaatttttt caaacaaaga catagtaacg
     8521 ttgtaacata tataaattta aaaagagctt agaaaataaa attattgaat ataaaatgga
     8581 aaaatataaa ttacacaaag agatatgagt acgtactaac aaatacgaaa atgtataaga
     8641 aattataaaa aagtacatca agaaaattta agatgtactt tttagattgt atttagactt
     8701 ataattatat agttaaatga ataacaaaga attaatctta gtactattaa atacaaaaat
     8761 ttttatattt tttctatcta aattcttctt tgtttaatat atattgaaga gtatttattg
     8821 cttctaaata tttttttctg tcttcttcag ttaatttaga aagtttttct cctaaaccat
     8881 cacttaattt atccataaaa tcagttgcta tcttatctcc ctcttcagta atgtcaatta
     8941 gatattttct tctatcaact aaatctcttt tccttgttac atacttctta tcagttaaat
     9001 catcaaccat actagttaaa ctgccttttt ctatatttaa cttagagcat aaatcagtca
     9061 tacttattac tccataattt tttatgaaaa ctaatgctct taattgtgtt ttatttacat
     9121 tataatctgg tgcaaatttt tttaggtctt ctaagtacag gctagaataa atcttaggaa
     9181 atgtttttac taaaaaac
//
LOCUS       DAEMGG010000144         9175 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEMGG010000144 DAEMGG010000000
VERSION     DAEMGG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.145"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..294
                     /locus_tag="KPK24_003269"
     CDS             <1..294
                     /locus_tag="KPK24_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG2300916.1"
                     /translation="IVPSRDEFITIYKQALIKKEIDLDMVNIRETFNVIPLKFFTILN
                     VFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNESLILNNLKNLEKQYNSSY"
     gene            307..819
                     /locus_tag="KPK24_003270"
     CDS             307..819
                     /locus_tag="KPK24_003270"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG2300917.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            893..3100
                     /locus_tag="KPK24_003271"
     CDS             893..3100
                     /locus_tag="KPK24_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG2300918.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3125..3574
                     /locus_tag="KPK24_003272"
     CDS             3125..3574
                     /locus_tag="KPK24_003272"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG2300919.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3589..4203
                     /locus_tag="KPK24_003273"
     CDS             3589..4203
                     /locus_tag="KPK24_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2300920.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4294..5850
                     /locus_tag="KPK24_003274"
     CDS             4294..5850
                     /locus_tag="KPK24_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG2300921.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            5840..7102
                     /locus_tag="KPK24_003275"
     CDS             5840..7102
                     /locus_tag="KPK24_003275"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG2300922.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7176..8963
                     /gene="aspS"
                     /locus_tag="KPK24_003276"
     CDS             7176..8963
                     /gene="aspS"
                     /locus_tag="KPK24_003276"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG2300923.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMLTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         3700 a          986 c         1761 g         2728 t
ORIGIN      
        1 atagttccat caagagatga atttataaca atttataaac aagcactcat aaaaaaagaa
       61 attgatttgg atatggtaaa tataagggaa acttttaatg taattccatt aaagtttttt
      121 acgatattaa atgtatttag agaattaaat ttacttgact ttaatttaaa ttatgaaaaa
      181 aattctgtac tcataagaat attgccaaaa ccacagaaaa aattagactt aaatgagagt
      241 ttaatactca ataatctaaa aaatttagaa aaacaataca atagcagtta ttaggaggcg
      301 tttcccatgg atttaaaaaa ctttataagg aatattgatg atttcccaaa gccaggcata
      361 gattttaaag atgtgacaac tctatttaaa gatggagatg catttaaata tgcagtggat
      421 tctatagttg aagaattgaa agataaagat gtagatttag taataggacc agaagcaaga
      481 ggatttttga tgggtacacc tgtagcatat gctttaggag taggatttgt tccaattaga
      541 aaacctggaa agttaccagg tgaagttgag agttatgagt atggattaga gtatggtaca
      601 gatactttag agatacataa ggatgcaatt aaaaagggtc aaaaagtagc aatagtagat
      661 gatttattgg ctactggtgg aactatggaa gccgctgcca agcttgtaga aaaattaggt
      721 ggagaagtag tttctatgca atttttgatt gagctaaaat tcttaaatgg gagagaaaag
      781 ttgtctaact atgatgttaa ttctcttata aaatactagt acttttataa tagttggtat
      841 aacaccaact attttcatat atattaaaat tttatacaaa ataggtggtt atatgcatga
      901 taaagaatta caagaaataa ttgaaaaaat aaaagtttat gcacctaatg caaattttga
      961 aatggttgaa aaagcctata atcttgcaaa aaaagcgcat gaaggacaat atagaaaatc
     1021 tggggaacca tactttattc atccattagc agttgcaaat attttaatag agatggaatt
     1081 agatattgaa actataacag caggtttact tcatgatgtt gtagaagata cagaatatac
     1141 ctatgaagac ataaaaaaag aatttggcaa agaagtagcg gatttggttg atggagtaac
     1201 taaacttggt caaataaaat atcgttcgaa agaagaaact caatctgaaa atttaagaaa
     1261 aatgtttttg gcaatggcga aggatataag ggttatactt ataaaacttg ctgatagatt
     1321 gcacaacatg agaacattaa aatacatgcc tccagaaaaa gcaaagtata aagcaaaaga
     1381 gacacttgaa atttatggtg gtatagctca tagattagga atttccaaga taaagtggga
     1441 actagaagac agagcattaa gatttatgga cccagaaggg tattatgatt tagttagtag
     1501 agtatcaatg aagagaagtc aaagagagga ttatattcaa ggaattgttg aacttcttaa
     1561 agataagttt aaagatttaa gtataccatg tgatgtatat ggtagaccaa agcattttta
     1621 cagtatatat agaaagatgc aaaaaaaaca taagactttt gaagagattt ttgatttaac
     1681 agcagtaagg atattggttg ataatgtaaa ggattgctat gcagtacttg gtatagttca
     1741 tacattatgg agacctattc caggaagatt taaagattat atagcaatgc caaaacccaa
     1801 tatgtatcaa tcattacaca ccactgttgt aggtcctgat ggagaacctt tagagataca
     1861 aataagaact catgagatgc ataatatagc tgaaaatggt atagcagccc attggaaata
     1921 taaagaaggt atctctagca aagagtcaaa ggttgaagaa aaacttcaat ggttgagaca
     1981 gatgatggag tgggaaaaag atttaaaaga cccacaagag tttatggatg cacttaaaga
     2041 agatgtattt aatagtcaag tttatgtatt tacgccaaaa ggagacgtaa ttgaattacc
     2101 agcagaatca acacctatag attttgccta tagagttcat acaaatgtag gtaataaatg
     2161 tgtaggagct aaaattgatg gaagaatagt tcctattgat tataagcttc aaaatggtaa
     2221 tattgtcgag gtacttacat catctaattc taatggacca agtagggatt ggcttaatat
     2281 agtgaagacc ccaaatgcaa aaagtagaat tagacaattt ttcaagaagg aaagaagaga
     2341 agaaaatgtt gaccgtggaa atacaatatt agaaagagaa tttaagaagc atgatatacc
     2401 acaaaaagac cctcttgttg aaaaatatat ggcattagtt gctaagaaat ttaatcagcc
     2461 aagtgttgag gacttaattg ccactgtagg ttatggtggt ataatgtcat ctcaagtggt
     2521 atctaaggta aaagacctat atttaaaaga ggttaaaaaa gctaaaaagg aacaacagat
     2581 acaagaagaa gatttaaata aacacaatat aagtgaaaca gaatacagta aaaagagaaa
     2641 gaaaaacgca tcacaaggaa ttatagtaaa agggcttgac aatatcttag taagatttgc
     2701 taagtgttgt aatcctcttc caggagatga aattgtagga tatattacta agggtagggg
     2761 agtagccata cacagagctg attgcccaaa tgcaaacaat aatggtgatt tcttccaaaa
     2821 tagattggtg gaggtttcat ggagttcagc aggaaaaggt aagtttgaag cggaaattca
     2881 aataaaagct attgacagaa gaggaattgt taatgaaata actcatattg ttgcaatgga
     2941 taaacttagt ttaaacggaa taaatgcaag aaaaggtaag gatagtattg taagtataaa
     3001 cttacttgta gaggttaatg acacagagga attaaaaatt ctaatgaaaa aattaaaagc
     3061 aattcctggt gtagaaagta tttatagaat gacaaattag agttaaaata atagggagga
     3121 aactatgaga gcagtagtgc aaagagtatc ttcatctaaa gttacagtag atgaaaacac
     3181 tataggtcaa ataaataagg ggttactagt attgttagga gtgactcatg atgataaatc
     3241 aagtgatgta gattatatga ttgataaaat attgaatctg agaatttttg aagatgaaaa
     3301 tgacaagatg aatttatctc taatggatat aggtggagag cttttagtag tatctcaatt
     3361 tactttatat ggagattgca gaaaaggaag aagacctgga tttagtaatg cagctagacc
     3421 agaacttgca aataaccttt atgaagaatt tgtaaaaaaa gcaaaagata aaggtgtgac
     3481 agttggtaca ggtcaatttg cagctcatat gatggtagag ttaactaatg atggtccagt
     3541 aacaatacta ttagactcaa gtaaatcttt ttaaggggga aaactttaat gattataaaa
     3601 aaatttgtag atagttattt tggagtaaat acttatgtat taggtgatga aaagactaaa
     3661 aaatgtgcag ttatagaccc aggtggaagt ttagtagaga cattaagttt tgtaaaagaa
     3721 aatgaattaa atattgaata tattatcctt actcatgggc atggagacca tataggatat
     3781 gttaaggata ttaaagaaaa aactggtgct aaagtagttg cacatgttga tgaaaaagaa
     3841 ttattaaatg acaaaaataa aaatctaagt tatacaatga gatgtggagc gcaagagttt
     3901 gatgcagata tctatgtaaa tgataaagac aagcttgaac ttggtgaatt aaaaatgact
     3961 ttcttgcata ctccaggaca tactcaagga tgtatgtgta taagagttga agatgaaatg
     4021 ttcacaggag atactttgtt tgctggaagt attggtagaa ctgatttata tagtggagac
     4081 tttaaccaaa tggaaaaatc acttaagaaa ttatgtaaat atgaagatgc tgttagagta
     4141 tatcctggac atggaccaac cagtactcta ggggttgaaa agaagacaaa tccttacatg
     4201 taattttata ttagtattta aaaaagtata aaatatttaa taactatatt agtgaggggg
     4261 ttcttctact gttaaataga aggagttaag ttgatgttgt atgttttttt aaaaggacat
     4321 gattataagt atgaagtagc agaacttata aaattgttta ctagtgaatt taagtttaaa
     4381 gactctaatg ctaacaataa tatagagtca aagcatttaa taaatagatt gatttatgag
     4441 aatggtgtat tattctcaag tactgaatac tatgaaaatg gaaacttaaa gtacgagtca
     4501 atacagaata tagaaaatat gaatcttgaa tttttagatg gatttgatag cagcaaaacg
     4561 attgaagaat ttcttaatag cctagatagt gaaagtaaga ggaactttaa aaagctgtgt
     4621 aaagaaaata tcaaaaaaag catgttttta gtgcttaaac aagtatttaa ttcttatgta
     4681 ccatggggaa ttttgactgg tattagacca gtcaaaattg tccacaattt aatggataaa
     4741 aagctagatg ataatagcat aagaaccatt ctaaaagata attattttat aatagatgag
     4801 aaaatagatt tagctcttga aatagctaaa agagagcgat tatttatgta tcctatagat
     4861 aaaaataaaa tttctcttta tgtaagtatt ccattttgtc caactagatg tgtatattgt
     4921 tcatttcctt caaattcatt aaaacaattt ggacatttaa aaagagaata tgtttataaa
     4981 ttgattgaag aaattaaggg gtttgccaaa gttattaagg atactaagaa agaagttgaa
     5041 actctatata ttggtggtgg aactccaact acacttgatg aggaagaatt ggatttgctt
     5101 ataaattcat tgttcaatga attagattta agtaaaatta aagaatttac tgtagaagca
     5161 ggtagacctg acactattac agaacaaaaa ctaagagtgt taaaaaaaca caatgttagt
     5221 agaattagta taaatcctca aactatgaat gatgatacct tggtaaagat tggaagagag
     5281 catagagtat ctgatttagt agattgcttt aatatggctc gtagaatggg atttgataat
     5341 ataaatatgg atataatttt aggtcttata gatgaagact taaatatggt aaagaacaca
     5401 ttggaggaga taaaaaaact ttctcctgaa agccttacag ttcatacatt agccataaag
     5461 agagcatcta acttaaatat aaatatggat aggtataaag aacatctaac acaatatgaa
     5521 gaaatggtaa aaatgataga tttatcaatg aaatatgcaa aagatatggg attaaatcca
     5581 tattatatgt acagacaaaa acatatgttg ggaaatttag aaaatatagg ttatgctaaa
     5641 gaaggatatg agtgcattta taatatacag attatggaag aaaaacaaag taactatgca
     5701 ttaggtgcag gtgctatatc gaaatttgtc tatgtagatg aagatagaat tgaaagagta
     5761 gaaaatgtaa aaaatgttga acaatacatt gaaagagtag atgaaatgat aaaaaggaag
     5821 aaagaagagg tatataaaaa tgttgactaa agctcctaga ggaactaaag acataactcc
     5881 aaaagaggct tacaaatggc gttatgtaga aaataaattt agagaaatat gtgctttata
     5941 tggatatgaa gaaatggtaa cacctatatt tgaacataca gaacttttca agagaagtgt
     6001 tggagatact acagatatag ttcaaaaaga aatgtattct tttaaagata aaggtgatag
     6061 agaaattacc ctaaagccag aaggtacagc aggtgtagta agagcattta tagaaaataa
     6121 attatatgca gatactcaac caacaaaatt attttatgtg acaccttgtt ttagatatga
     6181 aagaccacaa gctggtagac aaagacaatt tcaccaattt ggaatagaag cacttggaag
     6241 tgatactcct tcaatggatg ctgaaataat cgcactagct gttcaatttt ttaatgaagt
     6301 tggattaaat gatttggtag tgagtataaa ttcagttggc tgtccagttt gtagaaaaga
     6361 gtacaatgct ttattaaaag agtatcttga ctcaaaagct gatatattgt gtgatacttg
     6421 caatgagaga agagaaaaaa atccaatgag ggttatagac tgtaaaaatc ctacatgtaa
     6481 agaaaatatc aaagatatac catttattgc agaccatttg tgtgatgatt gtaaatctca
     6541 ctttgataaa cttcaagaat acttaaagga aatgaatata aactttgtaa ttgataaaac
     6601 aatagttaga ggcttagatt actatagaaa aactgctttt gaaattatat ctaatgatat
     6661 aggagctcaa agtactgtat gtggtggagg aagatatgat ggtcttgttg aacaattagg
     6721 aggaccaaaa ggtataagtg gaattgggtt tggactagga attgaaagac ttttacttac
     6781 acttgaaggt aatggaattg aaatagaaaa ccctcaatct acggatatat ttatagtaac
     6841 tataggtgaa gaagcaaata caagaagctt taaattgtta aaagacttga gacaaaatca
     6901 tataagtgca gataaagacc atatagagag aagtgttaaa gcacaattta aatattcaga
     6961 taaaataaat tcaaaattta caatagtaat tggagatgat gagcttaaaa atgacactgc
     7021 gacattaaaa aatatgaaaa catcagagca aactactgta aaattaagta cattagttga
     7081 agaattaaag caaaagctgt aaaataagaa agtatgatgt tttgtgtatt agataaatat
     7141 atagcattaa aattaaataa aatggaggta gtatcttgga aactttaaag ggtttaaaga
     7201 gaactcacta ctgtggagag ttgagagaaa aaaacataaa tgaagaagtc gtacttatgg
     7261 gatgggtaca gaaaaaaaga aatttaggtg gtcttgtatt tgttgattta agagatacaa
     7321 gtggattatg tcaaatagtt tttgatacag atgtagataa agaagctttt gaaaaagctg
     7381 aaaaattagg ggctgaattt gtaatagctg taaaaggaaa agtatgcgaa agacaatcta
     7441 aaaaccctaa tatgctaact ggtgatatag aaatatttgc aactgaactt agacttttaa
     7501 acaagtcaga aactccgcct atttatataa aagatgatga tgatgtttct gaagcactaa
     7561 gattaaaata tagatactta gatttaagaa aaccatctat gcaaagaaat ttaaaattaa
     7621 gacataaagt aatgaatatt acaagaaatt acttatcaaa caatagattt tgcgaaatag
     7681 aaacaccatt tttaatagct ccaacaccag aaggagcaag agattatcta gtaccaagta
     7741 gagttaatcc aggaaaattt tacgcattac cacaatcacc acaattatat aaacaattat
     7801 tgatggtaag tggtatggat agatatttcc aaatagttaa atgctttaga gatgaagact
     7861 taagagcaga tagacaacca gagtttacac aaatagactg cgaaatgtct tttgttgagc
     7921 aagaagatgt tatgagtatg attgaaggtt tgcttgaagc gatatttaaa gaagttttag
     7981 atgtagaatt ggcacttcca cttcctaaga tgacttatgc agaagctatg tctaaatatg
     8041 gttcagataa accagatact agatttggat atgagttaac agatatatct gatgttgttt
     8101 gtaactgtgg atttaaagtg tttgctgatg ctacacaacc aggaaaaagt gttagaggta
     8161 taaacgtaaa aggcaaagct gacgacttta ctagaaaaca aatatcatca ctagaagaac
     8221 atgctaagac atatagagca aaaggtcttg cttggatgaa agtaggacaa gaaggagtaa
     8281 cttctccaat agcaaaattc tttaatgaag aagaaatgaa cgctatactt acaagaatga
     8341 atgctgaagt aggagattta cttttatttg tagctgataa aaactcaata gtatttgatg
     8401 ctttaggaca agttagactt gaagtagcaa ataggcttaa tctattggat aaaaatgtat
     8461 ataacttact atgggtaact gagttcccag tttttgaaga agatgaagaa acaggcacat
     8521 tctcagcaat gcatcaccca tttacatcac ctatggatga agatttagac aagttagaag
     8581 aaggtgataa gtcatcttta agagcgaaag cttatgatat tgtattgaat ggttatgaaa
     8641 taggtggagg aagtgttaga atatcaaatt ctgatgttca atcaagaatg tttaaagcac
     8701 ttggttttac agaggaaaga gctaatgaaa agtttggtta tttattagaa gcatttaaat
     8761 atggtactcc tcctcatgct ggtctagctt ttggtcttga tagacttgtt atgttacttg
     8821 caggaactga taatataaga gaagttattg cattccctaa aaatcaaaat gctgtttgcc
     8881 ctatgacaaa tgctcctaca ttggcggaag atgagcaatt agaagaatta agtataaaag
     8941 ttgatataaa agataatgaa taatatgtag ttgcaagaaa ctaaaatgta aaaagtctgg
     9001 atataaaata tatttatatt cagacttttt ttattagaat tattttatat tttttctcta
     9061 tatattttca taaattatac ttatattctt aaccaatata attaaaatac tatgtttaaa
     9121 aattttacat aaacttgatt attttagaaa ctacttctgt aatgagtaca cgatt
//
LOCUS       DAEMGG010000145         8812 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEMGG010000145 DAEMGG010000000
VERSION     DAEMGG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.146"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1903)
                     /gene="recJ"
                     /locus_tag="KPK24_003277"
     CDS             complement(<1..1903)
                     /gene="recJ"
                     /locus_tag="KPK24_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG2300924.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDIS"
     gene            complement(1916..3580)
                     /locus_tag="KPK24_003278"
     CDS             complement(1916..3580)
                     /locus_tag="KPK24_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG2300925.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(3878..4375)
                     /locus_tag="KPK24_003279"
     CDS             complement(3878..4375)
                     /locus_tag="KPK24_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG2300926.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(4404..5783)
                     /gene="scfB"
                     /locus_tag="KPK24_003280"
     CDS             complement(4404..5783)
                     /gene="scfB"
                     /locus_tag="KPK24_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG2300927.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(6088..6234)
                     /locus_tag="KPK24_003281"
     CDS             complement(6088..6234)
                     /locus_tag="KPK24_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG2300928.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(6264..6842)
                     /locus_tag="KPK24_003282"
     CDS             complement(6264..6842)
                     /locus_tag="KPK24_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG2300929.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            complement(7210..8640)
                     /locus_tag="KPK24_003283"
     CDS             complement(7210..8640)
                     /locus_tag="KPK24_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG2300930.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT         2794 a         1590 c          819 g         3609 t
ORIGIN      
        1 gtgatatatc tgacattgct ctataaaatc catttataga attagatatt attagtgtat
       61 ctttttcaaa ataatcaaat atatttatat ctgtatttcc atcaaataat tcaaaatcag
      121 acacagatat attatacagg ctttgagtat cttttggtat tatttttgac attaatttca
      181 atgaaagttt gtcattactt gcacttttag gtcgagccaa tcgtatatct ttaagtaaaa
      241 attgtacttt cctatttccc atatagttat tttcatctaa ttggaatagt acatctacct
      301 tgtctcctac tttatacatt gattttaaat gactcatatt aaatcctata cattcatagc
      361 ttttctcttt ttctatgctt aatttcagat gttgttgatt cttacctata actttcaaat
      421 cttttaaaat ataatttcta actataaatc taggattagg attatttaat ccaaagggtt
      481 ctaacttatg taattcttct acaagattta aatttattac attttcttgt aattcaaact
      541 caacatttac attctctatc atatcatctt cagttaaatt ataatctgca aatttattta
      601 tttcatttgc taaaatttct acattgtctg aatccaagga taaccctgct gcttgttcat
      661 gaccaccaaa cttagtcatc aagtctttgc actttatgag agcttcaaat atattaaatc
      721 cttttattga cctcgctgaa ccagtagcct ccccattttc tatacctaga agaattgttg
      781 gtttgtagta tttttcagtt aactttgatg ctacaattcc tattattcca tgttgccaac
      841 cctctttagc taaaaccaac accttgtcat cattgtatct gctattagac tttagcattt
      901 cttcagcttc atgatacatc ttagcttcta tcatctgtct ttcattattt ttttcttcca
      961 atatactagc tatttctact gcctcttctt gtgattgagt agtaaatagc tctactccta
     1021 agtaagaata tcccaatcga cctgaagcat ttatcctagg acctattgca aaacctatat
     1081 gagaagaacc tatcttatca ctttctacac cacaaacttt tataagctcc cttaatccaa
     1141 tatttttgcc ttctttcatt gattttaaac cattttttac aattattcta ttttcatcta
     1201 taagtggaac aatatcacaa atagttgcta gtgttactat ttccaaataa ttatacatac
     1261 tagttttaaa ttcttctttt ggagtcaagg cttgaatcat tttaaatgct acaccacatc
     1321 cacatagtga atcaaatgga taattacaat cttcttgttt tggatttatt actgcaaagg
     1381 ctgatggaat ttcactttga cattcatgat ggtctgtaat tataacatct atgcccaaat
     1441 cattcgctgt attgacctca gacactgatg taattccaca gtcaacactt ataatcaagt
     1501 cacatcccct agagcttata agcttaatag catcttcatt tataccatac ccttcttcta
     1561 atctattagg aatatagtaa tcaacattgt aacctatact cttaaaatat aagcacaaga
     1621 tagatgtaga tgatacacca tcaacatcat aatctccgta aatccaaatt ttttcgttct
     1681 tctcaactgc taactttatt ctatcaacag ctttttgcat atctttcatt aaaaaagggt
     1741 ctcttaaata atctaaagat ggattcataa atatttctga atctttttca gtacatatcc
     1801 ctctattttt taaaatttgg cagatttccg gggaaatatt aagttttttg ctaaattcac
     1861 tttcctctat cttaccttta tacttcaata tccatttttt caaatttaat tccccctaat
     1921 cttttttgta ttttgaccta atacttctta taactaagca aaggcctatt ataaaggata
     1981 taaagaaacc cgctattgca gtaaatttta tccatatatt attttctata tttggagatg
     2041 caattataag agaagaacct actataatag atgccaacac taaactcaaa gatatttgag
     2101 atgttagctc tattatacaa ttttctaacc tcgtcatttt aacatcttca atttgcattt
     2161 ttatattatt tctttctata ttttttaaaa ttgcttttaa ttgctttgga atagttttta
     2221 tgtccagtaa tatttcttct acattttgtc ttgaacttaa aacaacattt ttagggttaa
     2281 atttactctt ataatgatgt ttcataaact cttttcccat agaaccaaaa gaaaaatctg
     2341 tgtttaaagt tctacttgtt ccttctaatg ttataactgt ctttgcaaga gttacaaatt
     2401 gtgcaggcat gactatttta tactgtctaa aaaacctaaa tagttcattt aatatatctg
     2461 ttacatttat tttttctata gaaatatcat agtaataatg tattagataa agtaaatcct
     2521 gtctaagtcc cttaatatca gcctctccat ttaatgcatc catttctatc aatagatata
     2581 ttatcttgtc aacattcttt tctacacctg ctaatgcaat ttcatttaat tggtttaaag
     2641 ttttattatc aataattcca atcattccaa aatctatata tgctatctta tttttagcaa
     2701 ctacgaatat attacctgga tgtgggtctg catgaaaaaa tccatgagat aatacctgtg
     2761 taaaaaatga cctaactcct atttcaacaa tagtttttgt gttataacct agtctcctta
     2821 ttttttctac atcacttaac tttgtaccat taactttctc cattactaaa attttcttag
     2881 tattatattc tgaatatact ttaggtatat atacttcatc actattttta aatatcctag
     2941 agaatttagt tgcatttata gcttcaaatg tataatcaag ttccctcatt aattgagttt
     3001 gaaattcttc aatcatctgt actaaatcta aattaaaatc cttttttaaa tcttttaaag
     3061 tatttgcaat agttctaaga atctctaaat cagattttat tattttctct atatttggtc
     3121 tttgaatttt tacgattact tcttctcccg ttttaagtac accttcataa acttgaccaa
     3181 ttgaagctgc tgctattggt tcttctttaa attctaagaa tatttcttct atacttaaac
     3241 ccacttcttc tttaaatata tcaatagcta tatttgaatc aaatttctct acatcatctc
     3301 tgagttttga tatttcatca attatatcct ggtctaacaa gtcttttctt gtactcaata
     3361 tctgtcctat ttttatatat gtaggtccta gttcttctaa aactcttttc attctctcac
     3421 cagttgtcat attttttatt tcttctggtg ggtcaaatag tggtatttta taggcaattc
     3481 cttctatatt tagtctttct acaataaaac taaaaccata ctttattaaa acatatccaa
     3541 tttctctata tcgctttaaa tttctataac ttattctcaa atcttttcac tcctaagata
     3601 ggtataccca aaaacttaca ccttaattta tcgtatatga cattatttca ccaattgata
     3661 ttctaatatc ctatatcctc tttaatattt aattttatca catatactac caattttatt
     3721 aaaattattt aactcagtta atttttattt ttgacatttt atatctggtt taatcaaata
     3781 ctttttgaaa actaaaatat caacaaaatc taataaaata aattagtggt tgcctcaaaa
     3841 cagatttaaa tatgttttga aacaaccact ctttaaacta atatttttga ttttgtgctg
     3901 cctctaaata ccattcataa gattcatcaa tatatttttt atcttcttct gtaagctctc
     3961 taacaacctt accaggagaa cccattatca aaacacctgg tggaaacttc ttattagatg
     4021 taattagtgt acctgctcct atcagagtat attctcctat ttcagcatta tccaatatta
     4081 ttgaacccat accaattaac acattatcac taattttaca accatgaact atagacctat
     4141 gcccaactgt tacattgtta ccaattatag tttctgtatc tccatgcact atagaacaat
     4201 cttgtatatt agtgttttct ccaatagtta ttggtccttc atcaccccta actactgcat
     4261 tgtaccatat acttgaatct ttgcctattt taacatttcc aattacatct gctgattttg
     4321 ccacaaatac actttcgtca attaaaggtt tatcttctaa ataatctcta atcatatata
     4381 aacaactcct ttaaaatata aatttagttg gttgctcctt ctaacattaa cttagcttgt
     4441 atcattatag agcactctgt acgttttctt tgcatttcac atcctaattc atatggtttt
     4501 gatatgtcat tattaaagtt tatcgcattt gcatgacatc cacctgaaca gtaaaactta
     4561 ttccaacatt gtttacactc ttctttagta tatacatgag cttctctaaa catatttttt
     4621 aaattttctg gaagtactat ttcttcatca aatatatttg ccattttaaa ttcttcattt
     4681 ccaacaaatt gatgacatgg atatatatct ccatttggag ttacagaaag atactcattt
     4741 ccagctccac atccagttat tctctttatt acacatggac cttgatttag gtctatcata
     4801 aaatggaaaa atttaaatcc atctccttgt aattgtctat ctgcatactc tacagcaaat
     4861 ttttcgtact cttcaaatat cttaggtaaa tcttcttctc taagtgcata tggattactc
     4921 tcatctccaa caactggctc tacagaggtt aatttaaatc ctaaatctgc aaaatgcata
     4981 acatcttttg aaaaatctaa attatctctt gtaaaagtac ctcttatata ataatactta
     5041 tcttttgctc ttttttccac aagtttctta aatcgtggta atgtaatatc ataacttcct
     5101 ttatcattta aagttggtct catattatca ttaacttctt ttcttccatc taaacttaaa
     5161 actacattat gcatattttc atttatataa tctataatct catcatttag cagtacacca
     5221 tttgtagtta ttgtaaatct tatattttta ttataatctt tttctacact tcttccatag
     5281 tcaaccagtt gttttacaac ctcaaagttc atcaaaggct ctccaccaaa gaaatctatc
     5341 tctaggttct ttctacttcc tgaattagca atcaaatagt caattgctgc cttaccaact
     5401 tcaaaactca taagttcttt ttctcctcca aaatcacctt gagctgcaaa acagtattta
     5461 cattttaagt tacaatcatg agctacattt aaacataaag cttttactac aggctctcta
     5521 tgtacaaagc ttggatgata ttgatatgta tcttctgtat ataaaagacc ctctttttcc
     5581 aaatttaata tttcttcata tgcttcattt attttttctt cttgatattt tgattttaat
     5641 atctctaata tttcttcttt acttttttct ttataaaaat ctaacaaatc gtatgcaaca
     5701 tcatctaaca catgaacagc tccaccattc acatctaaaa cgatattata tccattcata
     5761 gaaaatttat gtatcatact cataccctac ctcctaaatt actatctata ctaaagtttt
     5821 ttattatttt aattaaactt tacccttcat tatatcataa gtttacataa aatacataca
     5881 atacaaaaat gacttactct ccgtaagtca tttcattaaa tacactatat ccccctaaat
     5941 tggcgcacta atatattacc aaataggtat taggctccta taaaataaat aaaataaatt
     6001 ttgtatctga ttttatattg ttttaatctt attaaaatat aataatttct tttttcctga
     6061 taattttgta taaataagtg cttgtattta atttgtcttc tttagattgc tgatttcttt
     6121 gggtacttta ggctgatgaa taatccaagg acaggtacta ttagctgaca aaatagcagt
     6181 gctaagtgcc aatgagctag caaacttcat aaatcttaca ataaactttt tcataactta
     6241 actctccctc atacatattt tacttatact ttagctttta acttacctaa tactaacatc
     6301 acaaaaatcc aaaacatggc aaatgcgcta tatgtgaaat aatctacaaa tatactcaaa
     6361 gaaagactta aaattgttat caataacact actgtagata taaatatagc agttttctta
     6421 taaaccaatt tttccctatc acttataggg ttactcctat gttctattgg gcacagaaca
     6481 caaattccaa cccaactaac acttgcaata atcataataa tatttttaaa taagtctata
     6541 tttatatttt ctataattaa gattgtagat aaaattataa aaataaaggt taataagcat
     6601 ctaaaataat tgtctgcatg ataccctcct gaaaattgtc ttattggaca ataacacatt
     6661 aaaaaaaata atacatatgt aaatttacca aataaaaaac ctataaacag catggtggtt
     6721 atattaacta tgaaagctat taatatttca aatccataag aatagacttt agattcatta
     6781 ttatctatca tattattaca aattaaaaca gatgtcattt tttcagcata tcttttaaac
     6841 atttttacct ccaaaaaaat tttacatata tagagtacat tcttttttga gaaaattcaa
     6901 tatactttac aaaactagtt gttaaaaatg taaaactggt caaaaaaaga cctatataat
     6961 tttcttctta ttatttatat aacaaaacaa ttataataaa acaattttat caagctttat
     7021 atgcataaaa agcctatgtc aaaatagaaa taaacctttt ccccattgtt aaaaggctaa
     7081 aaatatattt catttcatat aaaaagagta cctcacaaac taaaaagttc cttttgagat
     7141 acccatttta tttattacaa aatatcaaat tttatttatt tgcatatgtt ttctatatat
     7201 tttagccttt taactttgtt tttgttttac atactgtagt gcttttctat tctgttttac
     7261 tgcttcaaga caaacttttt ctgtttgatt atcaacaaat tctaaagctc tataattttg
     7321 ctttactgct tctaaacata ttttttctgt ttgattattt acatatttca acattctata
     7381 atcttgtttt actgctttta gacaaacttc ttctgtttgt tctttcacat atttaagcgc
     7441 ttcataactt tgattaactg cttctaaaca aatcttttct gtttgttcct ttacataagc
     7501 taaagcatta tagttttctc ttactgcctc taaacatatt aattctgtct gattttctac
     7561 atatttaaga atttttccat tttgctttac tgcttctaca aatattcttt ctgttggtat
     7621 ctttacataa cttaatattt cttttatttc aagttcttca aatgaacctc ttactgcttc
     7681 tatacatata ttttcagtct gtttttttac atattgcagt acttttccat tttgtctaac
     7741 tgactccaag caaatttctt ctgtttgctc ttttacgtac tgtaatgctc ttccatcaat
     7801 tcttactgct tccaaacaaa tctcttctgt ttgcttattt acatactgta gagccatata
     7861 acttcttttt actgcttcca tacatatttt ttctgtctgt tcatttacat aatacaatgc
     7921 aaagtcattc tgttttactg cttctataca tatttcttca gtttgctctt ttacacaaga
     7981 tagtgcactg tagttttctt ttacagcttc taaacatata ctattacttt gttcctttac
     8041 ataagctaaa gattttccat ctttttttac agcttctaaa catatggctg gagtttgctc
     8101 ttttattcct tttaatatct ctgcaacatc atttttagat gccctttcta taaatgctag
     8161 acatacatct tctgtttgct cttccacaaa tttaaatact tccatgacat cataagttga
     8221 tgcatttttt atagcctcaa ttagtacatt ttctgtctta ttatttatgt agtacaaagc
     8281 tctgtagttc tgttttactg cttctataca aatatcctca ttttgatttg ttatatactt
     8341 aagagattta tatgtctgtt ttactgcttc catacatact ttataagtct tgtcctttac
     8401 aaactcaatt atagtaccat cctgctttac tgctagcaga cacatctctt ctgtttggtt
     8461 ttctatgtct tttaactctc tgtaatcata ttttactctg cttaaatcat ttaaatgtac
     8521 tttgttatct tcctttaaat cttcttcatt attttctata tcatactcaa attcttcatc
     8581 atctaaatag tcattttttt ctggtaaaaa tatagattta attttattct ttatatccat
     8641 aaaattatcc tcctttaata tgattctatc attaaagtaa atcgttttta agcacttatt
     8701 taattaattt tatattaagt ttatttaaaa gtctattcat tgtcgataga ttatatttta
     8761 tattaataat tttatcttat atatgtgggg taataaagtt aatagtggac at
//
LOCUS       DAEMGG010000146         8581 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEMGG010000146 DAEMGG010000000
VERSION     DAEMGG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.147"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            122..577
                     /locus_tag="KPK24_003284"
     CDS             122..577
                     /locus_tag="KPK24_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG2300931.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            626..1210
                     /locus_tag="KPK24_003285"
     CDS             626..1210
                     /locus_tag="KPK24_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG2300932.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1215..1538)
                     /locus_tag="KPK24_003286"
     CDS             complement(1215..1538)
                     /locus_tag="KPK24_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2300933.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1690..2208
                     /locus_tag="KPK24_003287"
     CDS             1690..2208
                     /locus_tag="KPK24_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2300934.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2384..3052)
                     /locus_tag="KPK24_003288"
     CDS             complement(2384..3052)
                     /locus_tag="KPK24_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBG2300935.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3167..4582
                     /locus_tag="KPK24_003289"
     CDS             3167..4582
                     /locus_tag="KPK24_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2300936.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4805..5263
                     /locus_tag="KPK24_003290"
     CDS             4805..5263
                     /locus_tag="KPK24_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2300937.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5260..5844
                     /gene="clpP"
                     /locus_tag="KPK24_003291"
     CDS             5260..5844
                     /gene="clpP"
                     /locus_tag="KPK24_003291"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG2300938.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6156..7232
                     /locus_tag="KPK24_003292"
     CDS             6156..7232
                     /locus_tag="KPK24_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2300939.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7299..>8581
                     /gene="bclA3"
                     /locus_tag="KPK24_003293"
     CDS             7299..>8581
                     /gene="bclA3"
                     /locus_tag="KPK24_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBG2300940.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADGATGVTGPTGA
                     TGATGANGLVGPTGATGAAGTPGATGPTGATGPTGVGITGATGATGATGPTGADGATG
                     PTGATGNTGADGVAGPTGATGATGNTGADGATGPTG"
BASE COUNT         3273 a         1100 c         1581 g         2627 t
ORIGIN      
        1 aactaaaatt ttaaggaaaa ataacatatt tgtattaaat tttattgaaa tgattaaatt
       61 tatttactta atgtaaatta ttagctaaaa ttgtattaaa taattaagga ggtgagtagc
      121 tctggatgaa atagatttaa aaataattaa ttctctaaaa aagaatagta gggcttcaac
      181 ctctgaaata agcagacaaa ttaatttatc agtaccagca gtctcagagc gtataaggaa
      241 aattgagcag tcaaatttaa ttcaaaagta cactattcaa attaatcggg aaaaaacaaa
      301 atataagctt ttggctttta tatttatcaa tactgattta tctaaaagta tgaaagaatt
      361 taagaaaact atattggaat atgatactgt attagaatgt catcatattt caggagagta
      421 tgattactta ataaaagtat tagcagaaga tacaagtaat cttgaagagt ttatatcagt
      481 gactttgaag gaaattgggg ggattcaaaa agctaataca accattgttt tatctacaat
      541 taaagaagaa tttaatacaa taaaagatat tttttaaata atttactagt aaagtgttaa
      601 ttatatttta ggaggaaaat agaatatgaa agttttagtt tttttagcaa aaggttttga
      661 aactatggaa tttagtgtat ttgtagatgt tatgggatgg gctcgtaacg attatgggca
      721 tgatattgat gtagtgactt gtggatttaa aaaacaagtt atgagcacat ttaatataca
      781 agtattagtt gataagacga tagaagaagt atgtgttgat gattatgatg ctctagctat
      841 tcctggagga tttgaagaat ttggatttta tgatgaagca tatgattcat cttttttaaa
      901 tctaataagg gagtttaatt caaaagaaaa aataatcgct tctatttgtg tggcagcatt
      961 gccagttgga aaaagtggtg ttttaaaaaa tcgtaaagct actacatatc atttgaaaaa
     1021 tggtaaaaga caaagacagt taagtgagtt tgatgtaaat gtagttaatg aaccaattgt
     1081 tgtagataaa aatattataa cctcatattg tccagaaaca gctccacatg tagcttttaa
     1141 gcttttagaa atgttgacat ctaaagaaca aatggatgaa gttaaattag caatgggatt
     1201 taaactgtaa tggtttatag attttctaag taatcagaac cccagtcaca catggaatct
     1261 aaaattggaa taatactctt tccaaaatca gatagcgagt attctacttt tggaggaaca
     1321 acaggataaa ccactcttat aattaaacca tctgattcta attcacgtaa ttgttgagtt
     1381 aacatcttag gcgttgcttt aggaattaat ttttttaatt cattgaacct caatatagtg
     1441 tctttaagat gccaaagtat aagtgtctta tatttaccac caatcaatgc aagtgttgct
     1501 tctattgggc aactgtaatt atctaaacat tttttcattt ttgggctcct ttatttattt
     1561 taaagtatct ttttaggtac tatattacaa aaaagtgcat tattgtaata tagtaccata
     1621 gtgataaaat tatagcatag gttaaataaa tcatcaactt agcctaataa aacaataggg
     1681 ggtaaataaa tgaatttcgt tgaacttgca aaaaaaagat attcttgccg taattatcaa
     1741 gatagaaagg ttgaaaaaga aaaacttgag aaggttttag atgttgcaag aatagctcca
     1801 actggtggta atcgtcaacc tcaaagattg attgttattc aggaaaaaga aggtataaat
     1861 aaactttcta aggctgccaa tatatatgat gcgccattag caattcttgt atgtggagat
     1921 aaagacaaag tttggacaag accttttgat gggaaacaac ttacagatat tgacacaagt
     1981 atagttacag accatatgat gttacaggca actgaacttg gattggcaag tgtttgggtg
     2041 tgctatttta atccagatat aattagagaa gagtttagtt tgccagataa tctagaacca
     2101 ataaatatat tattgatggg atatgaatct aagataccag agagcccaga gagacatgaa
     2161 aaaactcgtg ttcctttaag tgaaattgtt tcttatgaaa ctctttaatt ataaaattaa
     2221 gttattcata ggataaatgt aataaagtgt ttgttataaa caaaattcaa aataaataat
     2281 atctacatat aattaatttt agtgaagtat atctagttta actagaatgt atacttcact
     2341 aatttattta ttcaaaatat ttatgaggat tttaatatat taactaaaat atatcaattg
     2401 aattaacttt taaatctgga aaagcacttg aaatatagtg ttttatatca tttaaatggt
     2461 cttgaatttg cttttctgat attttatcag gtccatatac aaaatacata atatttttag
     2521 tatcttttgt tatagctgtt cgattatcag ggccattaag aatactagat aatataccaa
     2581 tattatctga taaaaataaa tcatcttttg ttaaaatatg ttctttacca cctataccat
     2641 aaaaatgttc acttccattt gctaattcta ttttcaaagg taattccatt ttatcaaggt
     2701 catgacctgc tgttagaaga agatttttca attctgctaa aaacattgac tcaattgtta
     2761 cacctacatc tgggatacct tttgatttta atagaataga ttctaattga agcaatacag
     2821 ggtaagtttt cttgaatttt ttataatact ttatataact tgaaacaggc tctgttttaa
     2881 ttttttcttt gcgattgtat tctgggtgtt gttctataat atttttaatt gtagagtttt
     2941 taagaactaa aaaattttct ttattaggtt ttgaatgtgt aatatttata accataactc
     3001 caaatttaat attgggataa atttctttaa ctttatgtga tacctctaac ataatataga
     3061 ctccttttta ttttatattt tatgatataa tcttagcaaa ttaaaagtgg attttatagt
     3121 tccattttta tagtaatttt atagaaccac ttttggaggt attagaatgt ggattacgat
     3181 taatagaaat agtaatatat cattatcaag acaaatttat tgtgagataa aagatatgat
     3241 tctggatgga agcttaaagt ttgagcaaaa actgccatca tctaggactt tatcaaaaga
     3301 actatctgta tctcgtaata cagttttaga tgcatataat caattaattg cagaaggata
     3361 tttggaaaca aggaatggat atggaacaat tgtagctaaa ggaatatcag ggcataaagt
     3421 tgattttaag ttaaatgata cttttagaga tgaaaaagat ttacatacta ataaacaaaa
     3481 aaattatata gattttcgtt caggagttcc agaattaaaa ctatttccac gcaaagaact
     3541 ttcaaagcta tataaacaaa tactgtatga tttatcagat tcggatttca gatatggaag
     3601 tacagctggt gtatgggttt taagagtgga aatatctaag tatttatttc gtactagagg
     3661 aattagatgt aatcccaaaa acattatgat tgtttctggt tcaacacaag gtttatcttt
     3721 aatttcacag cttttataca aaagtaataa aaaggttgct gtagaagacc ctattcataa
     3781 aggcttatta aaggttattt catctattgg atattcagtt gttggaatta aagtagatga
     3841 taagggaatg aataccaact taataaaatc aataaagggg atttcttttg tttatactac
     3901 accttctcat caatacccat taggagggat tttacctatt caaagaagac tagagcttat
     3961 taaatgtgca acagagaatg attactatat aattgaagat gattatgata gtgaatttag
     4021 atatgagggt caaccaatta gttctcttta tgaacttagc ccaaataaag taatttacat
     4081 aggttctttt agtaaaatat tatctccagc tattcgtgtt ggatttatgt tattaccaga
     4141 caatatttta gaaagctata aaaccttaaa aatgtactct gatgttcata cagaaacaat
     4201 attgcaacat gtattagctg aatttattgg aaatggaggt ttagagaagt acatatttag
     4261 aatgaagaag ttgtacaata gaaaacgtaa gtacctcata aaagttttaa acacggaatt
     4321 taaaaatgaa tttaaaataa aaggtgaagc agcagggctt catatagttg ctcatttcta
     4381 taatgttaaa tttacggaaa gtttaattca taaaattttt gaagcaggta taaaagttta
     4441 tcctatacaa caatatgcaa ttgagaatat attagaatat gactatgaaa ttatactagg
     4501 ttatgctcat cttagttttg atgaaattga aaaaggtgta aaaatactca gcaaaataat
     4561 acatatgaat caaggtacat agataaaaca tgtagcaatc tacatataat agatgtaatt
     4621 aagtttaaac tttaaaagaa atatatatta atatttttgt ataaaaatac ctgatatttt
     4681 tttatcgata ctttattgct atacactaaa ttaagatagc aatttatatt aagaaaaaac
     4741 tttttgcata ggttataata cttaaaagga gaggatagag ttgaaaacaa atacagatgt
     4801 tattatggag ataattgatt atatagaaga aaatcttaca gaaaatctaa atttagataa
     4861 aatagcttat attgctaatt attcaaaata ccatttacac agaatgttta caaatgttgt
     4921 aggatgtact atccatcaat acatacagag gaggagactt actgaagcag ctagacaact
     4981 tatatttaca gacaaatata ttatagacat agctattaca gtaggatatt ctacacatca
     5041 atcttttaca ctagcattta gaaatttata taaagaatct ccacaagtat atagaaataa
     5101 agatgaattt tatcctattc aacagagatt tgttttaaat ctaaatcatc aaattcaaag
     5161 tgaaaaatgt atttcttatg caaatatgaa ttataaacaa agaagagatg aaatcaattc
     5221 ttatcttaac tttaatatta attgtgaggt gaaagtagca tgagtttagt gccacatgta
     5281 attgaacaaa caggtcaagg ggaaaggtct tatgatatat attcaaaatt attaaaagat
     5341 agaattatat ttataggaga agaaattaat gatgctattg caagtttagt agtagctcaa
     5401 ttactgtttt tagaatcaga agacccagat tcagatataa ttatatatat aaatagccct
     5461 ggtggctctg ctacatcagg atttgctata tatgatacta tgaattatgt cagatgtgat
     5521 gtatcgacta tttgtattgg tatggctgca agcatgagtg cttttttact tgcaggagga
     5581 acacatggaa aaagatgtgc tttacctaat tcagagatta tgattcatca gccaatggga
     5641 ggagctaaag gtcaagcaac agatgtaaaa atagctgtag ataatatatt gaaaataaaa
     5701 gaacgattag ataaaattct ttctgaaaat acaggtaaat caatagaaga tattaggaga
     5761 gatacagata gagataattt tatgactgca ctagaggcta aagagtacgg gctgattgat
     5821 tatataatga ataggaatga ataaagtatc aaagtatcat attaaaatgt ctgttttaca
     5881 aatatatata taatattaaa taagagtgtc tcaaaatgaa ctttttagtt tatgggacac
     5941 tcttttttgt atgaaattaa atatggtttt atcatttcaa caattattac caaatttcaa
     6001 gtttaataaa ttctactata aaaaatacat aaaagataag gttctttagt aactttctgt
     6061 cgtcaaaagt ttttataaat ttaataacaa aacaagagaa aataccatat attacttatg
     6121 tctgcaatat atagaagaca aaggagttgt acattatgat tacaataagt ttgtgcatga
     6181 ttgttaagaa tgaggaagaa gttataggca gatgcttaga atgtgtaaag gatatagtag
     6241 acgaaattat aattgtagat acaggttcta cagattccac aaaagaaata gtctcaaaat
     6301 atacagacat ggtgtacgat ttcgaatgga tagatgattt ctctgctgca agaaattttt
     6361 cattttctaa agcttctaaa gactatacta tgtggcttga tgcagatgac ataattttag
     6421 aagctgatag agaaaaatta ttaaagatta aagaaagctt ggatacttcc attgatattg
     6481 ttatggcaaa atacaatgta tcctttgatg aaaatggtaa cccaactctc tcgtattata
     6541 gagaacgatt gtttaaaaga agttctaatc ataaatgggt tgaccctatt catgaagtag
     6601 tacctctttt tggagaggta ttttattctg acattgcaat atctcataaa aagcttcata
     6661 gacaggaccc tctaagaaat cttagaattt ttgaaaaaat gatttcagaa ggaaaaacat
     6721 tggagccaag acaccaattc tattattcta gagagttgta ctataatgct aggtataaag
     6781 aagcaataga aggttttact aagtttttag actcatcaag aggatggatt gaggattgta
     6841 tcagtgcttg cagagattta gccacatgtt attacttaat taatgatgaa aaaagtgcct
     6901 tatattcatt gtttagaagt ttcgaatttg atgagccaag agcagaaatt tgttgtgaca
     6961 taggaaagca tatgtttgat agacaaaaat ataaagaagc cattttttgg tataaggtag
     7021 cattaacaag agataagaat gatactaatg gaggttttaa gtcaaatgat tgttatggat
     7081 atataccatc tatacaatta agtgtctgtt atgacaggtt aggagaatca gataaagcta
     7141 tttattatca tgaaaaaaca aaagaaatca aacctaacga tagtgctgtt ttgcacaatg
     7201 aaaattattt tagtaaattt aaaaatagtt gataggtcac aatcatacta aatttgacat
     7261 attatatata gagcttaaaa gctcaaatat atcaggaggt gcttttaata atgagtagaa
     7321 ataaatattt tggaccattt gatgataatg attacaacaa tggctatgat aaatatgatg
     7381 attgtaacaa tggtcgtgat gattataata gctgtgattg ccatcattgc tgtccaccat
     7441 catgtgtagg tccaacaggc ccaatgggtc caagaggtag aaccggccca acaggtccaa
     7501 cgggtccaac aggtccagga gtaggggcaa caggcccaac aggaccaacc ggtccgactg
     7561 gtccaacagg aaatacaggg aatacaggag caacaggatt aagaggtcca acaggagcaa
     7621 caggggcaac aggcccaaca ggagcgacag gagctatagg gtttggagta acaggtccaa
     7681 caggcccaac aggagcgaca ggagcaacag gagcagatgg agtaacaggt ccaacaggtc
     7741 caacgggagc aacaggagca gatggaataa caggtccaac aggagcaaca ggggcaacag
     7801 gatttggagt aacaggtcca acaggcccaa caggagcaac aggagtagga gtaacaggag
     7861 caacaggatt aataggtcca acaggagcga caggaacacc tggagcaaca ggtccaacag
     7921 gggcaatagg agcaacagga ataggaataa caggtccaac aggagcaaca ggagcaacag
     7981 gggcagatgg agcaacagga gtaacaggcc caacaggccc aacaggggca acaggagcag
     8041 atggagtaac aggcccaaca ggagcaacag gagcaacagg aataggaata acaggcccaa
     8101 caggtccaac aggagcaaca ggaataggga taacaggggc aacgggatta ataggtccaa
     8161 caggggcaac aggaacacct ggagcaacag gtccaacagg agcaacaggc ccaacaggag
     8221 taggagtaac aggagcaaca ggagcaacag gggcaacagg agcagacgga gcaacaggag
     8281 taacaggtcc aacaggagca acaggggcaa caggagcaaa tggattagta ggcccaacag
     8341 gagccacagg agcagcagga acacctggag caacaggtcc aacaggagca acaggcccaa
     8401 caggagtagg aataacagga gcaacagggg caacaggagc gacaggtcca acaggagcag
     8461 atggagcaac aggtccaaca ggagcaacag gaaatacagg agcagatgga gtagcaggtc
     8521 caacaggagc aacaggagca acaggaaata caggagcaga tggagcaaca ggtccaacag
     8581 g
//
LOCUS       DAEMGG010000147         8561 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEMGG010000147 DAEMGG010000000
VERSION     DAEMGG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.148"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            250..759
                     /locus_tag="KPK24_003294"
     CDS             250..759
                     /locus_tag="KPK24_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300941.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGRLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            786..935
                     /locus_tag="KPK24_003295"
                     /pseudo
     CDS             786..935
                     /locus_tag="KPK24_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1173..1604
                     /locus_tag="KPK24_003296"
                     /pseudo
     CDS             1173..1604
                     /locus_tag="KPK24_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1791..1984)
                     /locus_tag="KPK24_003297"
                     /pseudo
     CDS             complement(1791..1984)
                     /locus_tag="KPK24_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            2086..2592
                     /locus_tag="KPK24_003298"
     CDS             2086..2592
                     /locus_tag="KPK24_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2300942.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSALDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            2597..2873
                     /locus_tag="KPK24_003299"
                     /pseudo
     CDS             2597..2873
                     /locus_tag="KPK24_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
     gene            3142..3714
                     /locus_tag="KPK24_003300"
                     /pseudo
     CDS             3142..3714
                     /locus_tag="KPK24_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            3817..4632
                     /locus_tag="KPK24_003301"
     CDS             3817..4632
                     /locus_tag="KPK24_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG2300943.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALNFSNNAIIEQLDRKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            4713..5189
                     /locus_tag="KPK24_003302"
     CDS             4713..5189
                     /locus_tag="KPK24_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2300944.1"
                     /translation="MTTYKVNSIGTICNNEKGAFIQLEAPYILALRALDGFSHINVLW
                     WFSDFDNTNFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSAVEIIDIDYMSGLI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPQVPDWCSHWPHSLEESASFAWESEFNF"
     gene            5203..5766
                     /locus_tag="KPK24_003303"
     CDS             5203..5766
                     /locus_tag="KPK24_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2300945.1"
                     /translation="MSLQDYIFEKPVIETEHLILRTMNKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPELLFDKSKKSTKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKHYQGNGLMSEVLKNVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITRWCYG"
     gene            6021..6932
                     /locus_tag="KPK24_003304"
     CDS             6021..6932
                     /locus_tag="KPK24_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2300946.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKR
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     SEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEIWIIPV"
     gene            7010..7444
                     /locus_tag="KPK24_003305"
     CDS             7010..7444
                     /locus_tag="KPK24_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG2300947.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            7912..8561
                     /locus_tag="KPK24_003306"
                     /pseudo
     CDS             7912..8561
                     /locus_tag="KPK24_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         3044 a         1224 c         1664 g         2629 t
ORIGIN      
        1 cactaaaaat tgtaggtaaa tttgtcttat gcctttataa atatttctac agttttaatt
       61 actggacata tctattttta ttataaaaag cgattaaaaa tggagaatat cctcataaaa
      121 actatatata agcaaaacgg ttaaaggaaa aagaagaaat ttacttgaat gttaaaatgc
      181 gttgcttgaa gcaatgggca gttttgccta taatatcggt aacaactgaa agaaaggtgg
      241 ttagccctaa tgttaagcga aaacattaaa acaatcagga agtcaaaagg actttcacaa
      301 caagaacttg ctataaaact gaatgtagtg cgacaaacaa tatctaaatg ggaacaagga
      361 ctgtcggttc ccgattctga tatgttgatt tccatatcag aggtgcttga aacgcccgta
      421 agcactttac ttggagaaac tattgttgag tcggaagttg ataacttaaa agtgatttct
      481 gaaaaattag agattataaa cttgcaactt gcacaaagaa aaaacatgag aagaaaaatg
      541 tttcattggt tatttatttt attatgtgca gtcatattaa tagtttttgt ggtcttaggg
      601 agactaaata gtccttactt ggattggaat tataccgacc ctgaaaccgc tgtttttgga
      661 gtagcttttc atgcttttga gtggctattt gtcagattag taccgattgt ccttatagga
      721 gcaattattg gaatcttttt gacacgtaag aaaatatgaa gtaactctgc tttttataat
      781 acagcaagcg ttccttaaaa gcgtcattct acatggcaat cgaggacgac tatgtaagga
      841 agaacctttt tgatttccaa ctaagcgaag ttattgagga cgacagtagg cccaaaaccg
      901 cattaagtaa agtacaggaa gcaagactgc tggacttata ctctcatgcg tcagatgcag
      961 gaaatgcgag gtctggtagc atgatttaca acgatttgta caacgactac gagtagaaat
     1021 atatggagat aagtattatc tacgaaatca gaaaagaaat gacttgataa tttactcatg
     1081 ttataattat tgatgtgaaa ttaataaatc gaaattcctt ttttggattt aacaatactg
     1141 agtaaggaaa aagtgaatta cttttgcaag gaggtgttat ttggatggtg ctagcaggta
     1201 ttttgattgt agtacaaggc tttttgactg gtcctttagc aaaaaaaatt ggtgagaagc
     1261 gactaatact gataggtatg ttttgcaaag ccttagtagc gtttgccatg gtattgacaa
     1321 ctgggtttgt tagtgtgttg gtggtttttg gcttatttgc ggtatcttct gctattacag
     1381 tacccacatt aaacgcaacc ctttcaaaaa ctgacaatca gcataaagga acactgatgg
     1441 gatttgccag taccgccgga aatcttagca aagttattgc tcctttatta actgggtatc
     1501 tgtatgaaag caatattgaa ttaccatata taaccacggg agggatagcc ttaatcggag
     1561 tggttatctg ctatatctgg ataaaaaatc aaaagaacaa atgatggaag aggacgttta
     1621 tatggaggtt atacacagta actaatctgg tatagaaatt caattgaggc ttttctgctt
     1681 tattaaagtc ttgctactac ttaaccctat aatatcctta attcattatg acaaattcca
     1741 attcaacagt tttgatacac ataaggaggt aacaatacta tggaaaatta tcaatgaagt
     1801 gctaattctt tatcagtgaa gttatccata agtttttcta cctgtaatcg tctggtgctt
     1861 tttgccagat cattggtagg taagaaaaat tcatcaacag acacatgaag taatgataca
     1921 aggtcacttg tatgcttgga tgttgccctt tattttcaat attagttaag taacgtgggt
     1981 caatatttgt aattcgattt tagggaaata tttaaagaaa tattttcgtt ttatttcttt
     2041 aaatcttcaa gaatattgat agttttttga aagtaggtgt gtattatgaa gaatgagaaa
     2101 aaaattacag tcgcattgct cattgtttat ttattgattt tatcatggat tattttattt
     2161 aagctacaat tttcattttc tgcgttagac cacataagac aaataaactt aattcctttt
     2221 ggtggttcag ttattgcaaa tggaaaaatt gacttcgatg aaattataaa caatgccatt
     2281 gtttttatcc ctgtaggtgc atatttcagt ttgcttttta aaaataaatc tgttctaaaa
     2341 gcaataggtt ctgttttagg aattagcttt gtatatgaga taattcaatt tatatttgct
     2401 atcggagcaa gtgatattac agacttaatt agcaacacat tagggggtat aattggaatt
     2461 gcattagtat atgtattatc tattgttcta aaggataaaa cacataagat attaaatcga
     2521 atagcaatag tatgtactgt attagtagtt gccttcttat ttatgcttct tgctgtaaat
     2581 aatatatttt aatgccatgt taattacatg ttgttttgga aaagacttta atctgtcaaa
     2641 gtttgctttt gaaattgaac atgaaattac accaatggaa caaatgaaag ctataaattt
     2701 ttcattggag aaagatgact gtacaagtaa agtattaata tacaatccag acaaatggaa
     2761 atatgtagaa tactatttct ataaagatag acctattaaa aaaactattg gaaaaattta
     2821 ttcaattttg catttgtcac aataaaatat gttttgaaat attgaatact taaatatgca
     2881 aaaaaacaaa tgtaactggc tatggggcta tatctactaa aaagctatgt gataggttgg
     2941 gattaaattt aaacatcact tctagaaggc atgaatacac aaggctgaca aactatacag
     3001 ttatgattaa acgataccaa cgggattgaa aatttcgtga agaactttga gaagtcctat
     3061 tacacatgag caacagcaac gaagctattg aagcaaggac gcaggcaatc tagcggttat
     3121 ctgacaaagg agttgtagga cttgaatgta gatactatca gaacggatat accgaccagc
     3181 tctgcggtta cagatgtgat tgtgtagaat atcgagcagt cgggaacgga cactttatct
     3241 gctacctacg aagtagatca gcagataaaa gagagagaac agacaacagc ggtcaaggca
     3301 acctataccg taaaagtcca tgtggacgct gacggggata tggtaatcgt tcagaacctt
     3361 actcttgcac cagcaatcga aaaatcagac tatgagccaa agatatcaga aacagacgca
     3421 agcgtggacg ctgataccgt taatgacgct accgcatttc tggaaacatt ctttgaggtg
     3481 tacccgacag ccacagaaaa agaacttgcc tattatgtat cggaaaatgt gattgaacct
     3541 atcggcaggg actaccttta ttctgaattg gtaaacccag tctttacaaa ggacggggac
     3601 aatgtgaaag tcaaggtagc tgtgaaattc cttgataatc agacaaaggc aacgcaggtg
     3661 tcgcagtatg atcttgtact ccaaaaggat agcaactgga agattttagg ataacatgga
     3721 aatgggcttg tatcataatt atagtgtgca agcccttttc atattgaagt atcaataatc
     3781 aataattata attaggtaca tgaatggagg gataaaatgt tcaaaattgg tgagttttca
     3841 aaattgacac aagtttctac tcgtatgttg cgttattatg atgaaacggg tttattaaaa
     3901 cctgctaaaa ttgattcatt aactggatat cgtatgtatt cagcagaaca aatctcaact
     3961 ttaaatagaa ttatatattt acgtgatagt ggcttcaatg tcgctgaaat tgcattagcc
     4021 ctcaatttca gtaataatgc cattatagaa cagcttgata gaaagtctat tgaaattatg
     4081 gataatatac aaaaagaaca agaaaaacta aagaaaattg agattgctaa aaaagaagtg
     4141 ttacatggca aaagtgaatt gtattttaac ataaccatta aagagatacc gagttattgc
     4201 gttttatctt taagaaaaga aataccaaat tattattatg agggcgaact ctggaaagaa
     4261 ctctctgctt ttgctaaaga aaataaaata aatatttctg atgaaacatt ctcaatatat
     4321 cacaatgaag aatataaaga aaaagatgtt gatgtagaat tatgtgccat tgtaaataaa
     4381 ataggaaaag ctacaggaga ttttaagttt cgttttaccg aaccagtttc tatgatggct
     4441 tgtactatgg tatatggaga tttttctaat atttcttatg cttatcgttc atttgcaaaa
     4501 tggcttcaag aaaacagtca atataagatg aaaggttcaa gccgtcaaat agttcatcgt
     4561 ggaccgtgga acgagaaaaa tccagagaac tatctaacag aaatccaaat tcctttagaa
     4621 aatctcttgt aaaaaatgat aatctgcctt gactttaaca tggtgttaag gtttatcgtt
     4681 tatttatcaa gaagaaaacg gaggaaaata taatgacaac ttataaagta aattcaatag
     4741 gtactatctg caacaatgaa aaaggtgctt tcattcaact agaagcacca tatattctag
     4801 cattaagggc attagacgga ttcagtcata taaatgtttt atggtggttc agtgattttg
     4861 ataatacgaa ttttagaagt gtattggaaa cagaacagcc ttataaaaat gctccagaga
     4921 aaatggggat ttttgcaaca aggtcgccag tacgcccaaa tccgattgct ttatcagcag
     4981 tagaaattat cgatattgat tatatgagtg ggctgataca aattgcttat attgacgcta
     5041 atgacaaaac gcctatcttg gatattaaac catacacgcc gagtttggat agagtggaaa
     5101 atccgcaagt tccagattgg tgtagtcatt ggcctcacag cttagaagaa tctgcttctt
     5161 ttgcatggga aagcgaattc aatttttaag gagtgatgaa ccatgagttt gcaggactac
     5221 atttttgaaa aaccagtaat tgaaacagaa catcttattt taagaacaat gaataaaaat
     5281 gacatagatg atttgaaaga atggattagt gatatttctt tatacgagta ttgggggaaa
     5341 cgtccctcaa aaagcgaaaa aaatccagaa ttactctttg ataagtcaaa aaaatctact
     5401 aaaagttttc attggggtat tgtccataaa caagatagca aagtgattgg tgaaatatgg
     5461 atatatctta ttgaaaataa tagaatggca aaagtggctt tcagactttc taagcattat
     5521 caaggtaatg gactaatgag tgaagtgttg aaaaacgtaa ttgaattttg tttttcaaaa
     5581 acagaattac aaaaaatatg gacagatgtg catattttaa atcatgcttc ttacaagaca
     5641 ttggagaaag tcggttttca aagggaagga catattcgag gggggaaaat ggtaaatacc
     5701 tattgcgact actacctata tggaatttta aaatctgatt atatcactag gtggtgctat
     5761 ggctaatatt gaagcgacga ttggcgaagc aatgaacaat acagttccaa aggtctatga
     5821 tacgaaaagc attaaaagat atgtgatgaa tttattagat atggctcatt aaaaagtacc
     5881 aaagaaataa aagttaccat atagacaaaa ttggtttttt atatcttttg atttcttata
     5941 agataatggc attgtgcgac aagctatgca aaaaattttc atgtatactt tgattataat
     6001 ttatacaagg aggtactgat atgtacgcat atgaaaatat tgaaatgtca ttaaactata
     6061 ttgaacaaca tttaagtgag aaaatcgaaa cagagaaact ggcggaaatt gcctgtcttt
     6121 ctacatttta ttaccaacgc ttgtttaaaa ggttagttaa gaaatcagtt caagagtata
     6181 tcaaacttcg ccgtttagca atggtaatag cagaattgaa taattcagag gaaagaatat
     6241 tagacatagc tttaaaatat ggattttcag accatgctaa cttcacaaga gcatttaaag
     6301 aagtttacca tattactccc gatgaataca gaaagaatgt tcctatgtta aatacatttg
     6361 acaagccaga actttcttca cgttatatca tgattgatga aaatgtccca ttgattgtag
     6421 gaaatattgt tttggaaatt caaagaaaaa cattaagaac aaaggaaaca tattttggtt
     6481 ttgaaaaaac agtaaatatt tctgaacaaa ttcctgctgg agaaagtaca ggaattgatg
     6541 ttccaggtca gctatggcgc gaattccatg caaagaaact gcaaaccgaa aatgccattt
     6601 ttaacaatga tattgaactt ggtgtatctc acttagcaag tccagataaa ggaacattcc
     6661 tttactttgc aggaggtatg gtttcatcaa cagatgtatg tcctaaaaat atggtacaac
     6721 atacattacc agcaggtgag tatattgtat gcaaaattga ggcagaatct tttgaagaac
     6781 ttgttactac tgctttaaat caagcaaata agtatctgtt tgaaacttgg cttccaaatc
     6841 ataatttggt tacaaagcca ttcatggcag aaaaatatta caaaggaaat gcagagatta
     6901 attatatgga aatatggata attcctgtgt aaacagtaaa tgctaaatag gaattatctg
     6961 acgtaataaa aaggaaatag aaagcttata aaggagggca tagtccgttg tgataaacat
     7021 aaaagatata aattatgtac ctaatattag tgaaataagc gtttatattg aaaactctgc
     7081 atttgatgag ttgtatcagc atatgaacaa tgaatataaa gcagtttgca aaatcgaata
     7141 cagtaaagat gtctgggcac gaggttggaa cgtaaagttt aaaaaatctg ggaaatcatt
     7201 gtgtgttgtc tatccgaaga agaactactt tactgtattg gtggttgtag gtaataaaga
     7261 gaaagaagat gttgaaaact tgttacctag gctttctgaa tatgtacagg aaatttatcg
     7321 taacactaga gagggaaatg gacaaaggtg gcttatgata gacttaaaat ctaaaaataa
     7381 tgtatatcaa gatgtattaa ggcttattga aattcgttat gccataaaat gtgtaaaacc
     7441 gtaataaaaa tatttttcac atacgtaatc tcaaggtatt atcgttgaga caagtaacct
     7501 atataattat gggaaatgag atttttagat acttaagaaa gtactttagg gggcttgcgt
     7561 tatggaaaga tttaagtaca attccacaca atcaaataca atctaataaa actttactaa
     7621 aaattgaatg caatacagct ttaaaggctc gtacaaacta tatgttttgt gcgggctttt
     7681 ttcatttaat aggaatttgg attcgtcaga gagtatatta taaaaataaa acgataaggg
     7741 taccttaaaa ataaggtacc tcaggctgta gacaaagtcc cggactctcg tctgggcttt
     7801 tcttatattt gagttatcca caattcaact ttttaagagt tatcttatta agaaaaaagt
     7861 gtatgttttc caccctatag ttttacttct gtgatatttg cagatgccac tcaagtagaa
     7921 agtggtggta cggcagaaga tgtcatgcag agttcagaaa gtctgggact accgcctaat
     7981 tccttagaca cggaaagctc aatcaagcag gggtgtaagt attttgcgtc cctgctttct
     8041 tcctgcaaaa atcaaggtat cgacgatttg aatgtagcga tacaatctta caactatggc
     8101 gggggttatg tgggctatgt ggcaggaaaa gggaaaaagc acacttttaa tcttgcagag
     8161 agcttcgccc gtgagaaatc gggtggaaag aaagtaacct acgccaaccc gatagccgtt
     8221 gcaaagaacg ggggctggcg gtatggctat ggaaatatgt tttatgtgga attagtcaat
     8281 cagtatttaa ctgttccgca ggtgtcgggg gaactggcac aaaaggtaat gaatgaagcg
     8341 ttgaagtatc aagggtggaa atatgtgttt gggggaagca atcccaacac ttcctttgat
     8401 tgtagcggac ttgtgcaatg gtgctatgga aaagctggta tctacttacc gagaacggca
     8461 caaacacagt atgacgcaac ccagcatatc cctttatcgc aggcaaaagc tggggagttg
     8521 ttctttttcc attcaaccta taacgctggt tcgtatgtaa c
//
LOCUS       DAEMGG010000148         8473 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEMGG010000148 DAEMGG010000000
VERSION     DAEMGG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.149"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            111..1397
                     /locus_tag="KPK24_003307"
     CDS             111..1397
                     /locus_tag="KPK24_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG2300948.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1509..1982
                     /locus_tag="KPK24_003308"
     CDS             1509..1982
                     /locus_tag="KPK24_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1641..1643,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG2300949.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2014..3324
                     /gene="grdB"
                     /locus_tag="KPK24_003309"
     CDS             2014..3324
                     /gene="grdB"
                     /locus_tag="KPK24_003309"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3061..3063,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG2300950.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3580..5112
                     /locus_tag="KPK24_003310"
     CDS             3580..5112
                     /locus_tag="KPK24_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG2300951.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5123..6268
                     /locus_tag="KPK24_003311"
     CDS             5123..6268
                     /locus_tag="KPK24_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG2300952.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6578..7657
                     /locus_tag="KPK24_003312"
     CDS             6578..7657
                     /locus_tag="KPK24_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2300953.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7770..8369
                     /locus_tag="KPK24_003313"
     CDS             7770..8369
                     /locus_tag="KPK24_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300954.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
BASE COUNT         3283 a          973 c         1697 g         2520 t
ORIGIN      
        1 ataaaaacac aataatatat ctttaaaaaa ataagataaa ttgtaaaatg atataatttt
       61 gagaaaaact caaatgcata tacacaaatt aaataacagg aggtgcaagt atgcgtcttg
      121 aattagggaa aatctttata aaagatattc aatttggtga tgtaacagaa gtaaaagatg
      181 gagtattatt catcaataag caagagatgc tacaagaaat tggtggagat gagcatatta
      241 aatctataga tatagaatta gctcgtccag gtgaaagcgt aagaataaca cctgtaaaag
      301 atgttattga gccaagagta aaagttgaag gtaatggggg aatcttccct ggtatcatgt
      361 ctaaagttga cactgtagga gaaggaaaaa ctcatgcttt aaaaggtgta gctgttgtaa
      421 ctactggaaa aatagtaggt ttccaagaag gtataataga catgactgga gaaggagcta
      481 agtacactcc attttctaaa ttaaataatg tagtagtaat agctgaacct attgatggtt
      541 taaaacaata cgctcatgaa aaagctgtta gaatgatagg attcaaagca gcaatgtact
      601 taggagaagt agcaagaaat cttactccag atgaagtttc agtatatgaa actaaacctc
      661 ttcttgaatc aataaaagaa tatccagagc ttccaaaagt aggatatgtt tacatgcttc
      721 agactcaagg tttattacat gatacttatg tatatggagt agatgcaaaa caaatagtac
      781 caactttatt atatccaaca gaattaatgg acggagcaat tgtaagtggt aactgtgttt
      841 ctgcatgtga caaaaaccca agttatgttc atataaacaa tggggttgtt gaagacttat
      901 atgcaagaca tggtaaagat ataaactttg taggtgtaat tataactaat gaaaatgttt
      961 atctagcaga taaagaaaga tcttcaaact ggacttctaa attatgtaaa tatttaggat
     1021 tagatgcagt tatagtttca caagaaggtt ttggaaatcc tgatactgac cttataatga
     1081 actgtaagaa gatagaaatg caaggtgtta aaacagttat agttacagat gaatatgctg
     1141 gacgtgatgg aggttctcaa tcacttgcag atgcagatgt aagagctgat gctgttgtaa
     1201 caggtggaaa tgccaaccaa gttgtagttc ttcctaagtt agataaagtt attggacatt
     1261 tagaagtagt agatgtaata gctggtggtt ctgatggaag tttaagagct gatggaacta
     1321 ttgaagttga aatccaagct ataacaggtg caactaatga aacaggattt ggtcatttaa
     1381 ctgctaaagg atattaagaa gtattttatt aaacgattga gtaatgttaa taaaacatat
     1441 tatagataat tataataagt gttatagata tataaaaata tattagaaat ataataagga
     1501 ggattattat gagtttactt agtaataaaa aggttcttat aataggtgac cgtgatggta
     1561 taccaggacc tgcgatagaa gaatgtgtaa aaacagtaga aggagcagag gttgttttct
     1621 catctacaga atgctttgtc tgaacagctg ctggggctat ggacttagaa aatcaaaaca
     1681 gagttaaaga tgctgctgat aaattcggag ctgaaaatgt tgtgatttta ctaggtgctg
     1741 ctgaagccga agctgcaggt cttgcagccg aaacagtaac tgctggagat ccaactttcg
     1801 ctggaccact tgctggagtt gccttaggat taagtgttta ccacgttgtt gaggaaccaa
     1861 taaaatcatt atttgatgaa agtgtatatg aagaccaaat aagtatgatg gaaatggttt
     1921 tagaagttga agaaatagaa gaagaaatgt ctggtataag agaagaattt tgtaaatttt
     1981 aatgcctaaa agcaaaaggg gtgaaactaa gagatgggaa aactaaaagc agtacattat
     2041 attaatcagt tctttgctgg tataggtgga gaagaaaaag ctgatactaa gccacatgtg
     2101 gcagaaactt tacctccaat aagtttacaa cttgataaat tattaggtga agatattgaa
     2161 atagttggaa cagtagtttg tggagatagc tatttcaatg aaaatataga tagtgcaagt
     2221 gaagaagttt tatcaatggt aaaaggtttt gaaccacaac ttttcatagc tggtcctgca
     2281 tttaatgctg gtagatatgg ggttgctgct ggaacaataa ctaaagttgt aaaagatgct
     2341 ttgaatatac cagctttaac aggtatgtat gttgaaaatc caggagcaga tatgttcaaa
     2401 aaggatgttt atgtagtaga aacttctgat tctgcagcag gtatgagaaa ggcacttcct
     2461 aagatagcta aacttgcagt aaaacttgct aatggagaag aaattggaac tcctaaagat
     2521 gaaggataca tagctagagg aataagagtt aactacttcc atgaagacag aggttctaag
     2581 agagctgttg acatgttagt taagaaaata aaaggtgaac catttgaaac tgaataccca
     2641 atgccaaact ttgatagagt tgacccaagt aaagctgtaa aagatttatc aaaatgtaaa
     2701 atagctttag ttacttctgg tggtatagtt ccaaaaggaa atccagatag aatagagtct
     2761 tcttcagctt ctaaatatgg aacttattct atagcaggag ttatggattt aacagaagag
     2821 acttatgaaa ctgcacatgg tggatatgac ccagtgtatg caaatttaga tgcagataga
     2881 gtattacctg ttgatgttct tagagattta gaaaaagaag gtgtaattgg aaaattacat
     2941 gagactttct atacaactgt aggtaatggt acatctgttg caaactctaa aaaatatgct
     3001 tctgaaattg gagcagcatt agtggctgat ggtgtagatg cagtaatatt aacttctaca
     3061 tgaggtactt gtactcgttg cggtgcaacg atggtaaaag aaatagaaaa aactggactt
     3121 ccagtagttc atatgtgtac agtagtacct atttcattaa cagtaggggc taatagaata
     3181 gttccaacta tagcaatacc tcatccactt ggaaatccag cgcttgaccc tacagaagaa
     3241 aaagccctaa gaagaggcct tgtagaaaaa gcattaaatg ctttaactac agaagtagat
     3301 ggtcaaacag tatttgaaaa ataaaaataa cacacacata tattatatag ataaaaatat
     3361 aaacacacac taaaaataaa aaataactat acacaaaaaa ataatataga tttcatgaat
     3421 ggataaattg gagttattca ttcatgaaat cttaaaaaat gccaaaatac ataaacaata
     3481 atagaatcaa atatacaata aaatacaatg attagagctt ttaataagtt taataagatt
     3541 aaaaaataag tgttcataaa atttatggag gtgcacaaaa tgacttatcc agttttaaaa
     3601 ggggcaggct atgtacttat tcatactcca gatatgatag tgcaaaatgg aagtacttgt
     3661 acagttgaaa gagcaacaaa tccagattca gaatttttaa aagaagtaag taatcatata
     3721 agaagttatg aagatgtagt taactacatg ccaaatcaag tttatatagg aaatagaaga
     3781 ccagaagaat taagagattt accaatgcca tggtgtgaac aaaagattga gggaactaga
     3841 aatggtaagt ttggagaaat aatgcctcaa gatgagttca tagctttaat gcaaataagt
     3901 gatgcatttg atttagtaaa attatcacaa gaatttattg atgaagtaaa gccaaaaatc
     3961 gaaaataact accctgaaat tgcaccattt gtaggtaaat taaagggtga tgatatagaa
     4021 gaaggtaaag aattagtagc aacacacata gctgaaggat tataccatga tggaaaattt
     4081 gttggttatg taaaaagagc acatgatgta gatgttaact taaatgccca tacaatgttt
     4141 gaaaatctag tagtaaaagc atctggagtt ttatctgcta ttcaaatgct aagacatagc
     4201 aaaatagacc ctgctgaaat agattatgtt attgagtgtt cagaagaagc ttgtggagat
     4261 ataaaccaaa gaggtggagg aaactttgct aaatcaatag ctgagatagc aggacttcaa
     4321 aatgcaactg gttctgacac aagaggattt tgtgcagcac caactcatgc attaattcaa
     4381 gcagcagctt tagtaaaagc tggcatacat aaaaatgtaa tggtagtagc aggtggagca
     4441 agtgctaaac ttggaatgaa tgctaaagac catgttaaaa aaggtcttcc tgtacttgaa
     4501 gatgttgttg gaggatttgc agtacttgta tctgaaaatg atggtgtaaa tccagttata
     4561 agaactgatt taacaggtaa gcatactgtt ggaactggtt cttcaccaca agcagttatg
     4621 acagcactta taacttcagg tcttgataga gctaatttaa aaataacaga tgttgatgtg
     4681 tattcagttg aaatgcaaaa cccagatata actaagccag caggagcagg agatgttcca
     4741 gaagcaaact acaaaatgat aggggcactt gctgttaaac gtggagactt agaaaagaaa
     4801 gaattgaaag attttgtttc taacaaagga ttaccaggat gggcaccaac tcaaggacat
     4861 ataccatcag gagctcctta tataggattt ttaatagatg atttaactac aggaaataga
     4921 aatagagcta tgatagttgg aaaaggtagt ttattcttag gaagaatgac taacttattt
     4981 gatggagtat cttttatagc tgaaagaaat acaggtgtta ctgaagagac ttcaggaata
     5041 tctaaggatg aaattaagaa gataatagct gaatctatga aaaaactagc attagatatg
     5101 ttagaagaat aggggtgagg atatgtcaaa aaaagtaatc gcagatgtat tcttagaagt
     5161 agctaatgca atagaaagcg gcgaatttgg caaaaaagta aaaattggtg ttactacact
     5221 tggaagtgaa catggtgtag aaaacatggt taatggtgca caattagcaa aatctaattt
     5281 atttgatatt gtgcttattg gaccaaaagt ggaaactgac cttgaagtag ttgaagttaa
     5341 tgatgaaaaa gaaatgcatg caaaaatgga agaattatta gattcaggat atattgatgc
     5401 ttgtgttact atgcattata attttccaat aggtgtatct acagtaggta gagtaataac
     5461 tccagcaaaa ggaaaagaaa tgatacttgc aacaacaaca ggaacaagtg ctactaatag
     5521 aatagaggca atggtaagaa atgccattta tggtattgct acagctaaat caatgggtaa
     5581 caaatgccca aaagttggaa tacttaatgt tgatggagca agacaagttg agaagtgctt
     5641 aaaagaatta aaagataatg gatatgacat ggagtttgcg gattcaatta gagcagatgg
     5701 tggatgtgta atgagaggta atgatttact tgtaggtgca cctgatgtta tggttactga
     5761 tacattatct ggaaatatat ttatgaaagt attttcttca tatactacag gtggagatta
     5821 tgaagctcaa ggctttggat acggacctgg agttggagag gactatgata gaaaggtact
     5881 tatagtatca agagcatcag gttctccggt agtagcaaat gctttaaaat acgcttatga
     5941 tgttgtaaag ggagatataa gtaatgttgc aagaaatgaa tttgcaaagg ttaaaaaagc
     6001 aaaatttgat gacattatat cctctcttac taaaaaagaa gttaaagctg aaaaagtaga
     6061 agttaaaatg ccagataaag aaattgttac tcgtcaaata gctggtgttg atataatgga
     6121 tttagaagat gcagtatctg agttatggaa aaacggaatc tatgctgaaa gtggtatggg
     6181 ttgtacaggt ccaatagttt tagtaaatga agctaaagga gacttagcag ttgaaacttt
     6241 agttaaagct ggatatactg caaaataagt acatttttta tatgtaaata agaacatata
     6301 gttataattt tattgattat aattatatgt tctttttatt ttatatcttg tcaatattta
     6361 tatattaaca caatccattt atttgattct ctagctaatt tattatattt attcaattaa
     6421 aattaagctt tctaactaaa taaaaactaa tgaagtatta aaatgaaatt gataaaatat
     6481 gaaaaaattt taggaataaa gtatctgtta gataaaaata agattaatta gtgatataat
     6541 ataattaaga taaaatttat tatttggagg taaaatcatg agcagagtaa aaaatgtagt
     6601 agaattgttg gaaactaaag gtgttgatgc actatatcta acaaagaaaa ctaatgtaaa
     6661 ctatataagt ggttttcctg atgaagaagc atatgcagtg atttgcaaag atggaaactt
     6721 tttagttact gatagtagat atatggagct tgcagaaaag gtttgcaaag attttgaaat
     6781 tataaattgg cataattttg atagaagtgt ggcaaaggca gttaaaagtg tatgtgataa
     6841 agtgggtata aagaaacttg gatttgaaag aactaatata gtgtttgata aatatgaaga
     6901 acttaaaaat ctgattgaga aggataatgg agaattaata cctacagaaa atattgtaga
     6961 gactttgaga tatgtaaaag acaaagatga aatcaaaaat acaagaaaag cttgtgaaat
     7021 agcggataag gcattagaag aattgattcc acatataaaa gctggagtta gcgaaattga
     7081 actggctaca aggttagagt actttatgaa aatgaacgga gcacaaaata tagggtttga
     7141 aactatactg atatcaggag caaagacttc attgcttcat ggtaagccta gtgataagat
     7201 tatagaaaaa ggtgattttg tacttataga ctatggtgct atgtataatg gctatatatc
     7261 tgatacaact agaactttta tagttggagg agcttctgaa aagcaattag aaatatataa
     7321 tttagttaag gaagctcaaa atgtaggggt tgagaacatg aaagctggtg tacatgcgac
     7381 aattccagat gctgagataa gaaaagttgt caaaaaatat gaagactatt actatcaagg
     7441 tataggacat ggagtcggta gagatgttca tgaagaacct ttcattggta attatggtga
     7501 taaaatcata gaagagggat gtataataac aatggaacca ggtatttatt tccctggatg
     7561 gggtggagtt agaatagaag atactgtttt gattactaaa aatggtccag aaagacttac
     7621 taaattccct aaagatttga tgattttaga caaataattt aatttatata tattttttta
     7681 cataaaatat aacataatct cactattaag ctctatttac atattatagt aaagagggct
     7741 taattttatt gtgagaggtg atagtatata tgtataaaaa acataaattt ataaaaaata
     7801 taaaaaaagc ctggtataaa atcaaattaa ttgatagatg ccttataatt gtaatggcaa
     7861 tattaatgtt tcaatctata tataacttat ttgttaatga agtaaattca caagatacta
     7921 caactattga tgtagtagta agaaccactt cagcagctat atttgggtat tttttaagtg
     7981 ctaattttat aaagagacct ttaaggaagt atacaaatac tgatgtatct tcaaatcttt
     8041 ttatagataa taatttatcc ccaaaaggtc aagagtctaa tcaaaataat ataatgaatg
     8101 tcaaaaatac cattggattt acttcagaag ctgataatta tgaaaagaaa atacctatta
     8161 ataataatga aggttttgaa gaaggagaaa ctagtgaatt gcaaattatt atagctactg
     8221 taatttgcat agtagcatta acagtacttt ttgttgtaag aaattttaca actacaacat
     8281 cagcttcttt aggtactatt tcacagatga gagattttgt ctcaggatgc gtgggttttt
     8341 tgctaggttg tccaagtaaa cctgattaaa aaagtattat caataaaagg tattttaaaa
     8401 agtatcagta taataatatt tagatttaaa tgtatacaat tataattctg taacattcta
     8461 tattgtattt tta
//
LOCUS       DAEMGG010000149         8437 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEMGG010000149 DAEMGG010000000
VERSION     DAEMGG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.150"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(109..1785)
                     /gene="bclA2"
                     /locus_tag="KPK24_003314"
     CDS             complement(109..1785)
                     /gene="bclA2"
                     /locus_tag="KPK24_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG2300955.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGVTGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGENGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVTQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2163..2702)
                     /gene="hpt"
                     /locus_tag="KPK24_003315"
     CDS             complement(2163..2702)
                     /gene="hpt"
                     /locus_tag="KPK24_003315"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG2300956.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(3030..4337)
                     /locus_tag="KPK24_003316"
     CDS             complement(3030..4337)
                     /locus_tag="KPK24_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG2300957.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4762..6537)
                     /locus_tag="KPK24_003317"
     CDS             complement(4762..6537)
                     /locus_tag="KPK24_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2300958.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6534..6671)
                     /locus_tag="KPK24_003318"
     CDS             complement(6534..6671)
                     /locus_tag="KPK24_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300959.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6749..7318)
                     /locus_tag="KPK24_003319"
     CDS             complement(6749..7318)
                     /locus_tag="KPK24_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2300960.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7502..8137)
                     /locus_tag="KPK24_003320"
     CDS             complement(7502..8137)
                     /locus_tag="KPK24_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2300961.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2418 a         1677 c          953 g         3389 t
ORIGIN      
        1 tttattattt tatagtcttt tcatcatata aacctattaa atattattct atttattggt
       61 atatgaaatc atattgttta ttattttata atcttttcat catataaact attgtattct
      121 ataaactgat acatatccag ctgcagaagt acctgtcgtg cctgaaccaa atgtaaaact
      181 taaagtaaca gctgctgctc tagcatcaac taaaaagctt aaacttacac ttacatctga
      241 tgaagtaagt gtatttactg cttttgcaat aaagttattt tgagttactc cattaaagtt
      301 agtagtaatt gacataaatc ctgcaagtga aagtgtggca cgtataccga aagatacatt
      361 gtatatccct ccaactgcaa gatttatagt actacttgtt ggaaaagtta tattagttcc
      421 atttataaat gatgaattaa atgtcactaa tgtattattc actaaacttg aagaggacac
      481 tgtaaattgt gcattgtttg ctgctaacac tcctgttgct cctgtcgctc cggttggacc
      541 tgttgctcca tttgctccgg ttgctcctgt cgcccctgtt gctcctgtcg ctccatttgc
      601 tccggttggg cctgttggtc ctatcgctcc tgctactcca tttgctcccg ttgctcctac
      661 tgctcctgtt gggcctgttg ctccatttgc ccctgttgct cctgtcgctc ctgttgctcc
      721 tgctactcca ttttctcccg ttggacctgt tggacctatt ggacctgcta ctccattcgc
      781 tcccgttgct cctactgctc ccgttgggcc tgttgctcca tttgcccctg ttgctcctgt
      841 cgctcctgtt ggacctactg ctccatctgc tcccgttggg cctgttgctc ctactgctcc
      901 cgttggacct attgccccag ctactcctgt tgctcctgtc gctcctgttg gacctaccaa
      961 accatttgct ccggttgctc cggttgggcc tgttgggcct accaaaccat ttgctccggt
     1021 tgctccggtt gggcctgttg ggcctgttgg acctaccaaa ccatctggac ctgttgctcc
     1081 tactgctcct gttggaccta cctctccatc tgctcctgtt gctcctgttg ctcctattgg
     1141 gcctgttgcc ccagttgttc ctgttgctcc agtcgctcct atacttcctg ttggacctgt
     1201 tgctcctgta tttcctgttg ggcctgtagc acctgttact ccatttgctc cggttgctcc
     1261 tgtatttcct gttggtcctg ttattccatt tgctccggtt gctcctgttg ctccagttag
     1321 tccggttggt cctgttgctc ctgttgctcc tgtattccct gttggacctg ttattccatt
     1381 tgctccagtc gctcctgtat ttcctgttgg tcctgtagaa cctgttattc catttgctcc
     1441 ggttgctcct ttgtttcctg ttggtcctgt tattccattt gctccggttg ctcctgtatt
     1501 ccctgtcgga cctgttattc cattcgctcc agtcgctcct gtatttcctg ttggtcctgt
     1561 agaacctgtt gttccatttg gtccagtcgc tcccatattt cctgttggac ccgttattcc
     1621 attcgctcca gttgctcctg tatttcctgt tggtcctgtt attccatttg ctccggtcgc
     1681 tccagttaca cctctaggcc ccgtcggtcc tgttggacca gtagcacctg ttggccctgt
     1741 tggacctaca tcttgatata aacttggacc tgaaatatca ctcatataat taatcctcct
     1801 tttttaaagt tagagtaata ctctataaat attgagtatt actctatata attatatgaa
     1861 tcgacaattt cttatgtgat aatgtatcta tttgcttata tcaacatata tgctttgatt
     1921 cttaaatttc attacttttt tgtaaaatct tttacaatta ttagtactct tttacaatta
     1981 ttaatacaat taaaaaactc atttcccatc aattatttaa aattttttta ttatgctata
     2041 aaaaatagat aattaaattg cataaaatta atttaatact actttaatta ctttttaaat
     2101 aaaaaaacct caacacgatt aataaaaatc atattaaggc atccacaaaa ttatataatt
     2161 gcttatctat cttcatcagt aacattgaat atataaggaa catttctata ataatcacca
     2221 aaatcaaacc catatccaac cacaaactta tcttctattt caaatccaca ataatctggc
     2281 actaaatcga cttttcttct gcttggttta tccaaaagaa cacagcattt taaactagca
     2341 ggatttttag cttttagatg ttttaacaca aaatccatag taagtgcaga atcagtaata
     2401 tcgtctacaa ccaaaacatc atatcctgct atatcagtag tcacatctga aactacttta
     2461 acctttccag tactttcttc attgtttcca taactagatg tagtcataaa attaactcta
     2521 agtggtaagt ttatatttct aactaagtct gcacaaaata taaagctacc ttttaataga
     2581 gaaactacca taagattttt atctttataa tctttttcta taattgctcc taattccctt
     2641 aatttatctt taattttttc ttctgaatac aatacttccc atttttttgt ttctatatcc
     2701 ataaaaagac ctccaattgt aataatcctt cttaatttta taagagattg ctcacttttt
     2761 caatactctt attcaatatt agtcaatatt attgagtttt tatgttcatt caacaattat
     2821 taacaatgta tcttatattt taagtagttc atatatccac actaaattac atactatata
     2881 ttttagacaa aaaaagaaaa aacttgcatt aacaagtttt ttctattata cataaattta
     2941 tacataatca tattttatta gagggtacta attaaattta tttatgctat ttttgttata
     3001 cctcataata caaaacatat tataaagtac tagtcaactt tattcatatc attcattaca
     3061 tttaagatta tctcatctgt tatagacata cctttgtcac ttactctacc taagttttgt
     3121 atactttgct caacacttga accaactata ccatttagtg gtggtacaaa acaatcttgc
     3181 ttagctatta tagcactttg tatagctgca gatgctgcag aagataattt taatgcacaa
     3241 ccagctttcg caccgtcaca tatcattcca cttaagtcag ctatcatatt ttctatagct
     3301 ccttctattt gcttttcagt accattcata agccaagaga taccagcagt tgctccagta
     3361 gaagcagcaa caccacatcc acatacagca gataatcttc ctgtatagtt cttaatataa
     3421 ccagttacta gatgagatat agctaaagct tttgctagtc tttcatcaga ttgtgggaat
     3481 tttttattat aagcaacgat tggaagaatt gctgttaatc catgatttcc acttccattt
     3541 gaactcataa caggcatttt tacccctgcc attcttgcat ctgaagctcc agcagttaac
     3601 atcattgcat aatttataac atcattacct aaaagacctt cttctataga ctttttaatt
     3661 ccatatccta caccaatacc agttttttgt tttaatccat attcagccat ctcttcattc
     3721 atttttactc catctagtaa aaattctata tctttaaaat caattccttc tacatttttt
     3781 attaattctt gtatagttac tgtatccatt aaactttcag ctttttctgc cgcatcattt
     3841 gaagctgata cttttggttc attgtctaat aatacttctc catctttttc taagaacgta
     3901 aaattatcat gtttagttct tattctcact ttagcaatat gattttttcc ttttaacaca
     3961 acttctataa atactttctc tcttgtatca gcaggtgtta ttgatagtgg agtattatcc
     4021 atataatcct cagctatttt aacttcctct tttgttagag tttctaacac acttaatcca
     4081 ttttcagaat gtccacctac tatacctaga gctgctgcaa tttttaatcc taatctttca
     4141 gtacctggta tacctacgca cataccattt ttataaatac taggactaac taacatacga
     4201 ttttcaacta tttcttctcc taataattcc tttgcctttg cacatgctag agctaaggct
     4261 actggttcag tacaccctac tgctggttta acttctgcct ttaacatttc cactaattct
     4321 tttcttatat ctctcattat atataaacct cccaattaag tttagattta gtactcaaat
     4381 aattaattaa ataatttctt atctatattt atagcaaaat acatgccata ttttttatat
     4441 ctttttatat ttttagtctt atattctata accattggta tgactgcctc tatttatgta
     4501 ttttttttat ttgtatagag tatttatatt ctttaaaatg gagaaacata tgacaatttc
     4561 gcaaaaagac aaaaaaaagt ctcaaaatga gattctatat cattttgaga cggctcaaat
     4621 atattatttt ttaacttata aaaaccattt tttaattcat aaaaaaagat tctgcaaaac
     4681 acttatagta taagtttaaa gctttcttta aagttatgaa tttagaaaaa tttaaatttt
     4741 atatagcttt ttattatatt actatgaaat tatattgtat ttttcaattt ttctatataa
     4801 tgtagctctt gatattccaa gtgctttagc tgttaattct ttatcttttt tataatcttt
     4861 gtacttattt aaagctttta ttatctcaat cttctctagt tcatcaagtg gaattatcct
     4921 atcgctttta acttctaact cttctacaga ggtattctta attttttgag gaattacatt
     4981 cattgtaatt attgaagaag aagacatatt cacactatat tcaataatat tctgcaactc
     5041 tcttatattc cctggccact tatattccat catagcatca acaaccctgc tatctatgtc
     5101 aataacattt ttatctaatt tttttgaata ctctttaacc atatgcttga ttataagtgg
     5161 gatatcttct tttctctctc ttaaacttgg taattttata ggtataacat ttaatctata
     5221 gtataaatct tctctaaaag tgccattttg aaccattttt tctaaatctt tatttgtagc
     5281 agcaataatt cttacatcta ttgaaacatt tgatttactt ccaattttat taagttcctt
     5341 ttcttgtaat actctcaata actttgactg taaatgcaaa ctcatatctc ctatttcatc
     5401 taaaaatata cttcccttat gagctacttc aaacattcca agctttccac cttttttagc
     5461 tccagtaaaa gctccctctt catatccaaa taattcactt tctaataaat tgtctggtat
     5521 agcagcacag tttacagcta taaaaggatt atctactcta ttgctatggt tgtgtatggc
     5581 tcttgcaaat agctccttac ccgttccact ttccccagta ataagtactg ttgatgttga
     5641 tttagaagct attagggctt cctttttaga atttcttata gctacacttt ctccaattat
     5701 gttttccaat tttattctat aatctttatt catcttattg tagtttttta tagcatctct
     5761 tttatctata aaatcaataa catatccctt taactcattt tttaatacta ttttattgat
     5821 gttgtatatt cccttcgaat catatccgta tcttttataa aaaaaactac aggatttgtc
     5881 tttcttaaag ttatttatag aaggtttctt tataaaatct aattcgacaa atatgttttt
     5941 gccagttata ctatccttta tattgaaaag ctctttaaat tttgaattat atttatcaat
     6001 ttttccatcc acatcaatag acacaacagc tttgtccata ttgtttaaca gtgtctctaa
     6061 ttttttcttt tctaattcaa gctcataagt tttaatttga gcttttaatt tattagatat
     6121 taagtctgcc attttgccta aaaagtttat aagaccactt ttattatttt ctataatctg
     6181 tgcttgttcc tttgtaaagg ctataaggcc tataacacca tgagagactc catcacatat
     6241 aatgggacaa cacacctctg caaactctac acaagcccca cttttatagc attccctaca
     6301 tatatcacta ctctttgggt catcaattag tattgtaatc tgctctgtaa gtgatttttt
     6361 aaaagccgta tatttatcta ctttctcacc tattttgtct atatatactc ctgtacctgc
     6421 aatcctaaat aactccttat ttactatagt tacatctata ttgagtactg atactatagc
     6481 ttcagctata ttttgaatat cagattttat atattctaaa cctatcataa tcatcattcc
     6541 ttatttttct ttaacataag tatttgtctt atttctttaa gaatcaagta tatcttattt
     6601 aaagtaaatt ggattgacat caggattaca aagagcataa caattattat taactcataa
     6661 tttatttcca tctaaacatc tccttataac aaaagactag gcttaaccta gtcttttata
     6721 ttatatccat atttatattt taactcattt atcttctttt tcccacataa atatatttta
     6781 atttatttga ctctttaatc ataccatcaa agtttaatct tgaaaatata ttaaatactt
     6841 catgtggata gtgaatcttt gcaacagaac cacaaaattt atgtttaaga tagtttaact
     6901 taacttttaa attctttaaa tttaaatcct ctaatataac ctctccattg ccctttaaca
     6961 ctctttgtat ttcagatgaa gctttttcac aatctgtaat aacattaaat tggtcaacta
     7021 ttattatctt gttgaactga ttatcatcaa atggtagaaa ctctaactta cctttaacta
     7081 cattgcaatt ttcattatac actagagaat aacagacatc ttcataatta ctattctcca
     7141 gtatagttac agaagttcca agacctctca atctcttgcc taggtttcct atatgaccaa
     7201 caagcaatat tttatcacct acattatact caattgtatc caaaaaacat tctaattcat
     7261 cagatgtata tttagcttta accggctgtt tatttatctg acataatatg cttttcacaa
     7321 tgcttcacct cttcatttcc tcaattataa attatgtaca gattacatta taataaatgg
     7381 cgagcaacta ttcaagagat atttcattag tattttttta aacaatcttt atacatttta
     7441 atatgatatt ataccaaatt tattaggatt ttattcataa aaatcagtct attttttatg
     7501 tttattcatt gttattttca tccaattttt ttattttcga tatggatact tcatatgcca
     7561 tttttttgac cacattattt tcatctattt ttttctcata ttctctactt tgaaccctac
     7621 cccaaagctg tattctatca ccaaccttta gattctttgc aaatttagca tttctacccc
     7681 aaactattga tggtatataa tcagatttat tataaggtct gtttatagca actaataaat
     7741 cggttatttc ccttcctaaa ggtgtcttcc tatacacagg ttccttacat acatatccat
     7801 ctaaaaatat actattaggg tctttatttt cctcgtctat gctttttatt tctctgacaa
     7861 acactgtaag cactaatcta tttttattat ttaatgtttt gttatatgat cttaattggc
     7921 ctacgacatt gactaaattt tgtttatcaa ggtcaatatc ttggagaagt ctttctgata
     7981 cagtcattgg aagtatatca aaagaatcgc ttagtctgtt tatttttatt ttcatattat
     8041 aaaatttttc tccaaatatc tcatggctaa attctagctt attgtctaac tcccctctta
     8101 agtttactac gttattctca tctttaacag ctatcatatt ttatccctcc acaattgtta
     8161 attcttatat ttctggtatc agcttatata ataaatatat ttataaggga tgtattttat
     8221 tacaaatctt tgattgctgt ttaaaagttt ttatttagtt ataaatttca tttaatattt
     8281 cacttttata attataaaat tattatcttt ttgtaccaca tatactaatt aaactaccta
     8341 tttcagtttt gtattaatta taaaaatcct attatatagt taataagtca tatataactt
     8401 aaatttattc actctatata agcaacttca acgaatt
//
LOCUS       DAEMGG010000150         8020 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEMGG010000150 DAEMGG010000000
VERSION     DAEMGG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.151"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            169..1620
                     /locus_tag="KPK24_003321"
     CDS             169..1620
                     /locus_tag="KPK24_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG2300962.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1818..2459)
                     /locus_tag="KPK24_003322"
     CDS             complement(1818..2459)
                     /locus_tag="KPK24_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG2300963.1"
                     /translation="MTDNYIFDSYNNLVDGLSAYLGEGYEFILYKFNGVHYSINKIAN
                     GKYNTVKEGDIITDFDLALINKLENDADGDFISYFNRNKLGEPLKSTDIVIRGVDNKI
                     IGLLCIHFYLNTPLTRIIGSYIPEHNAMQSAPVLENIVEDTDEVILKSYQDAKVIVEN
                     DSNISSNDKNKSIVSILNESGIFKIKDSVVKVADLMGLSKNTIYMHLRNLNKN"
     gene            2933..4312
                     /locus_tag="KPK24_003323"
     CDS             2933..4312
                     /locus_tag="KPK24_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2300964.1"
                     /translation="MAQDKIVVEILENIGGKENIKEVAHCMTRLRIKVCDSSQVNQEK
                     LKKIKGVLGLVLKGEQYQIVIGPNVSQVYNTLNEVIGIIPEKDSKKTISEGKQNSIAK
                     ILDTIAGIFNPIVPALAGAGLIKAILALLVASKFLSADSETYIILNTISDGVFSFLPF
                     MLAYSAAKIFKMNTYVAVAIAGAMLHPNFINLIQQGLTKISFIGIPMSLIQYNGSVFP
                     IVLTIWFASYIEKGVDKITPKALKIILVPALTILFAAPIALIIIGPIAQMLGNYIAGG
                     ASILFERGGIFAGLIYGGIYSFMVVTGLHHGMVPVLVDGITRYGFNYISPVSGSSNMA
                     QAGSAFGVWLKAKDKDLKAVAATATISALTGVTEPAIYGVNLRLKKPFLCAAIGGAAG
                     GGFAAYFQCKAFAMGGPSFLTLPMFIGEGGHVGLVAIAFTIAFIVSAIATYIVGFEEE
                     VTADSHANL"
     gene            4356..5090
                     /locus_tag="KPK24_003324"
     CDS             4356..5090
                     /locus_tag="KPK24_003324"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010990049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG2300965.1"
                     /translation="MKIYLTKDYEEMSQVAAQMVLKYMYSNDNKRINLSITAGQTPKR
                     TYEILSEFVKNKPYFENVHYYNFDEIPIKGEKVGVTIRNLTNMYFREAQIPEERIHVL
                     DENNYQGFDRKIEADGGIDMVLMGLGWDGHFCGNLSGTVNSFDEETRAVSTYITDDMA
                     KLLTKSVGGNPEKLFDYYVTYGPKTIMHCKNIIMIVNGKHKAQILKQTLFGPLDLLCP
                     SSLLRLHPNFTLIVDEEAASEIKDLI"
     gene            5179..6378
                     /locus_tag="KPK24_003325"
     CDS             5179..6378
                     /locus_tag="KPK24_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2300966.1"
                     /translation="MYNHMKKVFQGLEGGLFSSVEKADVGDSYQKLGEQGVSLMGWAD
                     PFFPDYCIPRHILDAATLAISSNMSTHYTAPVGNDLLKIEIAEKLKEVNNLDVDFKRN
                     ILITPGSDSGLYYSMLPFINEGDEVLIPTPCYPNNIQNTKLMGGVPIFVELKESDGYQ
                     IDITEFEKRLTNKTKLVVLTHPNNPTTTIFNKESLEKLAKFIIDNDLILICDQAFEDF
                     TFENELITPASLEGMFERTVTIFSTSKGMGLSGFRVAYMVCSDKLMDKYYGAAVTVLG
                     ATNTVAQMSTIEAFKDRSFMEDFERVFDKRRQEAYKIINEIPNVSMQMPESGFLGWIN
                     VSKLGTSSEIQKYLVDEAKVCINDGINYGPGGEGHLRIILGSYKDDSKVIGALNRIKD
                     ALIKKQQ"
     gene            6402..6884
                     /locus_tag="KPK24_003326"
     CDS             6402..6884
                     /locus_tag="KPK24_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2300967.1"
                     /translation="MLSIFKSKKNDNKLKAFASGKVIPIEDVDDNVFSSKMLGDGIAI
                     YPTNEYVVAPCDAEVNIIMKDTKHAVGIKLFNGMEILFHVGIDTVSMNGEGFEIFVSP
                     GDKIKRGDKLISFNSKLIKEEGLQDVVIMVVTNSEQYKNIKYHIGLEAVIDDTIIAEI
                     "
     gene            complement(7202..7799)
                     /locus_tag="KPK24_003327"
                     /pseudo
     CDS             complement(7202..7799)
                     /locus_tag="KPK24_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2968 a          914 c         1355 g         2783 t
ORIGIN      
        1 ttgaatataa aactgcaata tattaaaaaa attattcatt ttaaaccttg taatttgatt
       61 ttataaatat tataattaaa ttaatgaatt tttaacaagg aattattcat ttttttgatt
      121 ttaagattta ttgagaaaac aaaactaaat aggggggtaa ttaatataat ggattttttc
      181 aatggtattt taacgagtat taacaatgca gtagtatttg taaacaatta cttatggtcg
      241 tatatcttaa ttataatgtt agttgtaatt ggtatttact ttactattaa aacaaatttt
      301 gtacaattca gatattttat agaaatgttt agacttctgg gtgatggtac agcgaataaa
      361 aatgcaaaaa aggaaggaaa aatctcttca tttcaagcat tttgtataag tacatcttca
      421 agagttggaa ctggaaatat agctggaata gcaattgcag tggtagcagg aggacctggt
      481 gctgtatttt ggatgtggct tatagcatta attggttctg catcaagctt tgttgagagt
      541 acattagctc agatttacaa agtgaaagat ggtcaagctt ttagaggtgg accagcgtat
      601 tatatggaac aaggtctaaa taaaaaatgg atgggtattt tattctcaat acttataact
      661 atatgttatg gttttgtatt taatgcagtt caagcaaata ctgtttcact tgcatttaat
      721 aatgcatttg gaataagtaa aatggctatg ggaataggtc ttggaatttt aactgcaata
      781 gttatttttg gtggagtgca tagagttgca aaagtttctg aaataatagt tcctgtattt
      841 gcaggattat atatattagt tgctttggct atagttgtaa tgaatataac agagatacca
      901 agtgttatag cattgatagt tgaaagtgca tttgatttta aaggaatggc gattggtaca
      961 tttatgggag ttgtaatgac tggagtgaag agaggtctat tttctaatga agcaggtatg
     1021 ggtagtgctc ctaatgctgc tgcaacagca catgtaactc atcctgtaaa acaaggttta
     1081 attcaatctt taggtgtttt tacagatact ataataatat gtagttgtac agcattcatg
     1141 gtattattat attcaggtta tagtagtagt ggagcaactg gaattgaatt agctcaagaa
     1201 gctttaactc atcatatagg accagttgga aatatattta tagcagtttg tatattcttg
     1261 tttgcattta gttctatagt tggtaattac tattatggtg aatcaaatat ggagtttatg
     1321 agtggaagta agactaaact taatgtattt agagtttttg ttgtaggtat ggttttattt
     1381 ggttcactta ctcaagtgga tgtagtttgg aatcttgcag acttatttat ggcattgatg
     1441 gcgataataa acttaatagc aatagcactt ttaggaaaat atgcatttat tgctttaaaa
     1501 gattatacaa gtcaaaaaaa atcaggaata aaagaccctg tgtttattgc agatgaaata
     1561 gaaggcctag aaaaggtatc agagtggcat agggaattat ctgaggaaca tttaggataa
     1621 aaaatagtat aaaattataa aaccattttg aaagactaga gtatgtatat actttatatg
     1681 aatataatat tgaattaatt tatattactg gaatttttaa ttccagtaat atttttttaa
     1741 ttacaaggat attatgatga cttaaagact gtggaaaact cttttttagt tttcattatt
     1801 aatattaatt gacaaattta gtttttattt aaatttctta agtgcatata aatcgtattt
     1861 tttgataatc ccattaagtc tgcaacttta acaacagaat ccttaatttt aaaaatacca
     1921 ctttcattta aaatagaaac aatagattta tttttatcat tgctagatat attagaatca
     1981 ttttcaacaa ttacctttgc atcttggtat gattttaaaa ttacttcatc agtatcttct
     2041 acgatatttt ctaaaacagg agcactttgc atagcattat gttccggaat atatgaacca
     2101 attattcttg ttagtggtgt attaagataa aaatgtatgc agagtagacc gattatttta
     2161 ttatcaactc cccttatcac tatatctgtt gattttaaag gttcacctag tttgtttcta
     2221 ttaaaataac tgataaaatc tccatcagca tcattttcga gtttattaat gagtgccaaa
     2281 tcaaaatcag ttataatatc tccttctttt acagtattat atttaccatt agctatttta
     2341 tttattgaat agtgtactcc attaaattta tataaaataa actcgtatcc ttctcctaaa
     2401 tacgcagata atccatccac tagattgtta taagaatcga aaatatagtt atcagtcatg
     2461 ttaaaagtca tatgttacct cctaagaaat atcttaatat catattaaca tataaaatga
     2521 atttaataaa atatatttca gtgaaataat aaatataatg aaaaaaaaac tattgactga
     2581 aaaatatttc agtgatatta ttaaaataca gtaaggcaaa cgtattattg acagaatata
     2641 taaaatgtta aaaatagtat ctaggaatta aacaaattaa aggactagtg atttgaattt
     2701 ttttagtata atatgtgact tccaattaat atttattaaa caccaataaa attgtaaata
     2761 tgcttaatga tatactttag cgatgaagtg aaaatatttt atttttatga ttaacctata
     2821 ggaattaatt tagcatactg tttagctagt aaatctaaat aaattttagc tttagctgat
     2881 atcatgttga ataaagtttc tattggattt tgtataacaa aggagtgggg agatggcaca
     2941 agataaaatt gtggttgaaa ttttagaaaa cattggtggt aaggaaaata ttaaggaagt
     3001 agcacattgt atgactagac tgagaataaa ggtatgtgat agttctcagg taaatcagga
     3061 aaaactaaag aaaataaaag gtgtactagg tcttgtatta aaaggtgagc aatatcaaat
     3121 tgttataggt cctaatgtat cacaagtgta taatactctt aatgaagtta taggaattat
     3181 acctgagaag gatagtaaaa aaacaatatc tgaaggtaaa caaaatagta ttgcaaaaat
     3241 attagatact attgcaggta tttttaatcc tatagttcca gcattagcgg gtgcaggtct
     3301 aataaaagct atattagcat tactagtagc atcaaagttt ttatctgcag atagtgaaac
     3361 ctatataata ttaaatacaa tatctgatgg tgtattttca tttttaccat ttatgttagc
     3421 ttactcagca gctaaaatat ttaaaatgaa tacttatgtt gctgtagcaa tagcaggagc
     3481 aatgctacat cctaatttca ttaatttaat tcaacaaggc ttaactaaaa tatcttttat
     3541 cggtattccg atgtcactaa tccagtataa cggaagtgta tttccgatag tactaactat
     3601 ttggtttgca tcgtatatag aaaaaggagt agataaaata acaccaaagg cacttaagat
     3661 aatattagta cctgcattga ctattttatt tgcagctcct atagcattaa ttattattgg
     3721 accaatagct caaatgctag gaaactatat agccggtgga gcaagtatcc tatttgaaag
     3781 aggaggaata tttgcagggt taatttacgg aggaatttat tcattcatgg tagtaacagg
     3841 attacatcat ggtatggttc ctgtattagt agatggaata acaagatatg gatttaacta
     3901 tataagtcct gtgtcaggta gttcaaatat ggctcaagca ggatctgcat ttggagtatg
     3961 gttaaaagca aaagataaag atttgaaggc tgtagcagct acagctacca tatcagcatt
     4021 aactggagtt accgaacctg caatatatgg tgttaattta agattaaaga aaccgttttt
     4081 atgcgctgct ataggtggtg cagcaggagg tggatttgct gcatatttcc aatgtaaagc
     4141 atttgctatg ggaggtccat cgtttttaac tcttcctatg tttataggag aaggtggaca
     4201 tgtaggattg gtagcaatag catttacaat agcatttata gtatctgcaa tagctaccta
     4261 tatagtagga tttgaagaag aggtaacagc agattcacac gctaacttat aataaagaga
     4321 aattatttaa catatcaaaa aggagattaa ttactatgaa aatttattta actaaagatt
     4381 atgaagaaat gagccaagtg gcagcacaaa tggtgttaaa gtatatgtac agcaatgata
     4441 ataaaagaat aaacctttct ataacagcag gtcaaacacc aaaaagaaca tacgaaatat
     4501 taagtgaatt tgttaagaat aaaccttatt ttgaaaatgt acattattat aactttgatg
     4561 aaataccaat aaaaggcgaa aaagttggag ttactataag aaatttaact aatatgtact
     4621 ttagagaagc tcaaatacca gaagaaagaa tacatgtgtt agatgaaaat aattatcaag
     4681 gctttgatag aaagatagaa gccgatggtg gaatagacat ggtattaatg ggattaggtt
     4741 gggatggaca cttctgtgga aatcttagtg gtacagtcaa ctcttttgat gaagagacaa
     4801 gagctgtttc aacttatata acagatgata tggctaaact attaacaaaa tctgtaggtg
     4861 gaaatccaga aaaattattt gattactatg tgacatatgg acctaaaaca ataatgcatt
     4921 gtaaaaatat aataatgata gtaaacggaa aacacaaagc tcaaatatta aagcaaacac
     4981 tatttggacc attagattta ttatgtccat catctttatt gagattacat cctaacttca
     5041 cattaatagt agatgaagaa gcagcatctg aaataaaaga tttaatataa ataagtaagg
     5101 aattaaaatt aaaaatgaat ttaggtgcaa agtaatattt tattttgcac ctttgcttta
     5161 ataaaaagga gaataaaaat gtataatcat atgaaaaaag tattccaagg attagagggt
     5221 gggttatttt caagtgtaga gaaagctgat gtaggcgatt cttatcaaaa attaggtgag
     5281 cagggtgtaa gtttaatggg atgggcagat ccatttttcc cagattactg tatacctaga
     5341 catatattag atgcagctac attagcgata tcaagtaata tgtcaactca ttatacagcg
     5401 cctgtaggta atgacttatt aaaaattgag atagcagaaa aattaaaaga agtaaataat
     5461 ttagatgttg attttaaaag aaatatatta ataactcctg ggtcagactc agggctttat
     5521 tactcaatgt taccgtttat aaatgaaggg gatgaggttc ttataccaac accatgctat
     5581 ccaaataata tacaaaatac taaattaatg ggaggagttc ctatatttgt agagttaaag
     5641 gaatcagatg gatatcaaat agatataaca gaatttgaga agagattaac taataagacg
     5701 aagttagtag ttcttactca tcctaataat ccaacaacta caatatttaa taaagaatct
     5761 ttagaaaaat tagctaaatt tattatagac aatgatttaa tattaatatg tgatcaagca
     5821 tttgaagatt ttacttttga aaatgaatta ataacaccag cttcattaga aggtatgttt
     5881 gaaagaacag taacaatatt ttcaacatca aaaggtatgg gattaagtgg atttagagtt
     5941 gcatatatgg tatgcagcga taaattaatg gacaaatact atggagcagc agtgacagta
     6001 ttaggtgcta ctaatacagt agctcaaatg tcaacgattg aagcatttaa agatagaagc
     6061 tttatggagg attttgaaag agtttttgat aaaagaaggc aagaagcata taagataatc
     6121 aatgaaatac ctaatgtatc tatgcagatg cctgagtccg gatttttagg ttggataaat
     6181 gtatcaaagc ttggaacatc atcagaaata caaaagtatc tagttgacga ggctaaagtt
     6241 tgtattaatg atggaattaa ttatggtcca ggaggagaag ggcatttgag aataatttta
     6301 ggaagctata aagacgatag taaggtaatt ggtgcattaa atagaataaa agatgcttta
     6361 attaaaaagc aacaataaat aaaaaataat tggagtggag tatgttatca atatttaaga
     6421 gtaaaaaaaa tgacaacaag cttaaggcat ttgcatcagg gaaagtaatt cctatagaag
     6481 atgttgatga taatgttttt tcatctaaaa tgttaggtga tggaatcgct atttatccaa
     6541 ctaatgaata tgttgtagcc ccttgtgatg cagaagtaaa tataataatg aaagatacta
     6601 aacatgcagt tggaattaaa ttatttaatg gtatggaaat cttatttcat gttggaatag
     6661 atactgtatc tatgaatgga gaaggttttg agatatttgt ttcaccaggt gacaagataa
     6721 aaagaggtga taaattaata tcttttaata gtaaattaat aaaagaagaa gggttacaag
     6781 atgtagttat aatggtagtt actaattcag aacagtataa aaatataaaa tatcatattg
     6841 gactggaggc agtaatcgat gatactatta ttgcagaaat ttaaaaacag ttatttaagt
     6901 aaatacttag ataatttaga tatagttaaa attaaatcct taaaatcaaa ttatttaatt
     6961 tttgtgtaat tttaactata aagccgatta ataatataaa gtatactaaa taatatggtt
     7021 attatgattg ttactgacaa agcctaagta gatataacaa tttgctctaa ttgagtattg
     7081 ctatatctac ttgtttttat atttaaaata aaagagtctg cattgaaaag ataattctga
     7141 tgagagtaaa tagggtattt aataatagtt taattctgac ttatcaagat gaacctattc
     7201 atatacctaa aacatcttta acaccttcta aattcataca aatagctgta taggctgcct
     7261 tattgattat ttttccgtca atttttactt tgtagtgaac agcatatata aatattatcg
     7321 aatagattgt atcaagagct ctattttgct attcattagc taaagatagt actctgtcag
     7381 ttatttttga aactaatgca tgagatatat caattccgta aatattattt aaatgtatga
     7441 tatatatcgc gagtcgacat tccttttgag tacatgccaa ttacttggtt ctctattcca
     7501 gaaatttcat tttcgtaatt ttgaataacc ctaggctgga aatcatccct agggatattt
     7561 aattttacct ctccaaaatc tgatttaaca ttcttagctc tatatccatt tcttgaattt
     7621 gatgttgctt tactttggtt atcatatctc tcgtacatta aatgatcttc aatctctgac
     7681 tctaacattt tgtgaattgt gcttgcgaat aaatctttta acatctcttg gatgtcttta
     7741 gtatttttta catcatactc ttgaactaag tgttttaata tttaattttt gttgtacata
     7801 aaaaatgtgt acctcctatt ttggtaatta gatttaatat cttgactttt aggagaatac
     7861 acattttatt ttacagaccc tcttttctag aaaagttttt atgaaaattg tgatataaat
     7921 tattaataaa aaataatttt atatttattt tattatgtaa tttagtttta ttaaaaattt
     7981 tttattattg gatatagatt atctattata aattttatct
//
LOCUS       DAEMGG010000151         7921 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEMGG010000151 DAEMGG010000000
VERSION     DAEMGG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.152"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            64..315
                     /locus_tag="KPK24_003328"
     CDS             64..315
                     /locus_tag="KPK24_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300968.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            549..968
                     /locus_tag="KPK24_003329"
     CDS             549..968
                     /locus_tag="KPK24_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300969.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            993..6050
                     /locus_tag="KPK24_003330"
     CDS             993..6050
                     /locus_tag="KPK24_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG2300970.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(6236..6973)
                     /locus_tag="KPK24_003331"
     CDS             complement(6236..6973)
                     /locus_tag="KPK24_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2300971.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(6992..>7921)
                     /locus_tag="KPK24_003332"
     CDS             complement(6992..>7921)
                     /locus_tag="KPK24_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2300972.1"
                     /translation="NNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFISCF
                     ALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQEYK
                     LQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFKSK
                     NSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDNAI
                     ESLSTIKKEKKYIRVHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGYGL
                     HLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
BASE COUNT         3003 a         1066 c         1126 g         2726 t
ORIGIN      
        1 taagttaata caaacaatta ttataacata ttttttaaat ttaatcgcat atggaggtat
       61 tttatgaata gatatacaaa aataattaat atgatgggaa gttattacac aaaagacttt
      121 gaaaaagaaa aaaagaatgt aataaaagtt agagaagtta aagaagatac agttagaaaa
      181 tttttcttac aaggtgactg tgaagttctt gtagtatttg aagacactgg gaaagaaata
      241 cttattgatg acttttctcc tgaagaagat atcaaaaaat atttaggagc aaaatttata
      301 aataaaaaaa gatagttatc aaaaataatt ctaaaattaa aatttaataa atgtacagtt
      361 ataattctat ataattatag ttaaaagtta tcttttattg cttttttaga ctttaagata
      421 gctttttttc tattttttgt aaaataattc cataattcac attaataatt cacaaaatga
      481 tataatataa caaatagata aatttttaaa tttctaaata cataattaat attttgcgag
      541 gtgattttat gcaaatttat tttagagtta aggctgttgg caaacgcaaa cctatgctag
      601 aacttacccc ttttgatgta tctgacaaca tcaattgcct aaaagaagct atctctgaaa
      661 ttgtatctaa aaatgttctt gaatataatg agaaaatccc agaaaaatca ataataaact
      721 tcttaactaa tgaagaaatt gaatctcaag ctgatattgg aaaagttgga tttggctcta
      781 tttacaatga gaataaacaa tctgtagaaa aggcaataga ggtagcgtta caggcatttg
      841 aagatggaat ctacaaagtg cttataaatg aagatgttat tgaaaaactc gattcctcta
      901 taaatctgaa aagtggagat atatttactt ttataagact taccttgctt gctggaagaa
      961 tgttttaaaa taaataattt ggaggtacat aaatgttaga acgtactaga gtagaaaaaa
     1021 atcctaaaat aattgaaaat tttaaaaata attttactaa atcttcttca aaacagtact
     1081 acgacttagc aaagtctctt tgggactgca tagaaaatag ttcatactat tatagagaac
     1141 ctgaagacaa attcttaaat atgataaaga aatactacaa ttgccactac tacgaaatta
     1201 aaaatatgga cgagctcctt tctggacctt taaaaaatgc attagaatta atatttactt
     1261 ctgaacaagt agaagaactt tgccaattaa ttaaaagaaa gatggattat acatattcag
     1321 gaggatatta tcgtaaaggg tatcgctcaa ataaacttgt ggattatttt agaggtatta
     1381 taaggtttat ttcaaactat gttgatttaa tttattatga tttttcagtt atagattatc
     1441 ttactcttcc aaataaggtc ttgtactatg actctataat gagtgataaa atagcactag
     1501 aaatagacaa aggaaatcaa aaagtaataa gtgctttaga agaagtaata tttggagaaa
     1561 atcaaactgc tctacttaca agacaaatga taattggtat catgaagagt catagtgata
     1621 aatgctatga aatgctttct aaacttcttt tggcagccaa acaacaagaa ggacttagac
     1681 agcaaattgt agagagttta gatgagggta caatttctgc atcaatatac atgttaaaaa
     1741 ctattttgga caacaattta gagcgattta gttctgttat aagagccttg gatacatgga
     1801 caggacttgg tttttcagac caaaaacctg gaataatacg taaatgttta gaactttcat
     1861 ataaatcatt gactgatgaa aagttccgta tagaatcaat taatagcaat gataatcttg
     1921 aaatttattt tgccttatgg gctactgcaa cttacaacat atatgactct tactctctta
     1981 ttgaaaactt actttcttgt caggaaaaat ataaaaaact tgtagctctt tactttttat
     2041 cgcatacaga aagtacatat tttaaacatt ctatagcatc taaaaacatt aagggtgatg
     2101 atttagagat actagcatgg gtaattacaa atttctatgt agaaggttct gttggctact
     2161 actatagttg gcaaaacaga gaaactaatt ttgacttgtc aaacactcct attgacaaag
     2221 actttgatgc tagaaaagaa caatttgaaa gtttaaaaca tactttagaa attttaaatt
     2281 caaagacaaa atcattttca ggaagtgtat ttccatggac ttggattgaa atttcttctc
     2341 atgaaatact taaatgtatg atgactttaa cagcatacga tatgaatgct caatttatag
     2401 atgtgcttat ggaaaattgt catcttatga gttcagattt aagagcttca cttatagtca
     2461 attttgtaaa tgaaccaaag acagatagac aacgtgaatt tttattggaa tgtttaagtg
     2521 ataagagtgt ttccaataga gaaacggtat tgagtctact tagaaagctt gagctaacag
     2581 atgtagaatt agataagata accaatctat tgtctttaaa gacaagctct attagacaaa
     2641 cagtaataaa aattttactt gaacaagatg attcaaattt ggaaaatatt gcttcttcac
     2701 tgataaaatc ttcaaattct caaaaatgcc ttggaggtct tgaaattgta aacttattaa
     2761 aagacaatac tgctagacaa gaactatata ataagcttgt tttacttata gaagatattg
     2821 ataaatctaa aaagaataca tctcaagttc aaatactaat tgataatttg ttaggtaaag
     2881 caaaaaatga ttactgcaaa gaaaatggtt ttggaatata taaccctgaa aacaaagttc
     2941 aaattccaga acaatttgat tttaaagatg attctaatag ctcatcaaag cttaataaaa
     3001 ttatctcaaa tataagttta tctactcgtg aggattctgt ttctgtagca acaaaaaaag
     3061 aagcaaaaaa aattctcgat acggatataa ataaaataat aaaagtttat gaaaaatttg
     3121 atgagcttat tactgaaaat gcagattatg aatatgaagt tgaaagatgg tcaggtaatg
     3181 tagacaaagt acttttaggt tattctactt ataacttgac tcctattaaa tataaagttt
     3241 ctaatgatac tcttgataat tatccattag cagagctttg gagagaaact agtaaaaagc
     3301 ttgacttaga ttacaaatta atacttgata ttttatttta tagtataagt acatgtggat
     3361 ataatgaaac agaaaaaagt tggttttcat ctatatattc aaatgtcctt gactctaaaa
     3421 aacaaaataa aatatataca aaacttgaaa aacttaaatt taatggactt acaagaaaac
     3481 ttttaagagt tttactaaat gagtttgacc ataaagaaat atttaactat tgtttagaaa
     3541 tatactataa acttagaaac ttattgacta tagaacaatg taaaaaacaa agagttgaat
     3601 ttactaaaaa ctattacaga caaaatgaat actgccctgc tttaaatgcc cctattttaa
     3661 actattggct tgataaagca cattcgttta tttatgatga tatttctttt actaagtttt
     3721 tctacactac gttttcaaat tatcagttgc tagaatataa gtttcctttc tatactagca
     3781 taaatgtgtt tgctcgtgca catgaattag gattaatgac tgaagagcaa gttaatagag
     3841 aaatgataag tggatacaac gcatcaaata taataagaac attgacaaat cctagatggg
     3901 ctggcgacac attagaaaag tacccttggc ttaaagataa tttaaaaact gttacaaacc
     3961 gaattataga aattgaatca cgtagaggtg atatgccaac agaggtgtct catctagcta
     4021 ataatataca atactttgaa ggtataaatt attttgtaaa tattttggtc gctcttggta
     4081 aagaaacgtt ccagagagga tattcatatg gtagcaatta ttcaaaaaaa gatgttttaa
     4141 gttctctact taaaaattgc tatcctacta aagaagatga ctcaaaaaaa ttaaaaagtt
     4201 tacttaaaaa taccgacatt actgagttaa gattgataga atctgctatg tatgcccctc
     4261 aatgggtaaa cataattgaa aaatatttaa actggaaggg tctgaaaagt acaatttggt
     4321 acttccatgc acatataaat gaaggcttct ctgcacaaaa agaatcagag gttgctctat
     4381 tctcccctat ttcaccacgt agtttcattg atggtgcatt tgatgtagat tggtttttag
     4441 attcatataa aactattggg gaagaaagat tcaatatact ttacaagtca gctaaatata
     4501 taactagtgg tggtaactct catagacgtg cacaactcta tgctgatgct acacttggta
     4561 agcttgataa agacatgttg atgaaagaaa ttgaaacaaa tagaaatcaa gataaattga
     4621 gaagtttctc tttacttcct atgaaaaaag gagacctaaa agaagcaact ttccgttatg
     4681 aatttattca aaagtttgct aaagaaagca agcaatttgg tgcacaacgt cgtgaaagtg
     4741 aatcaaaagc ttgtactata gcgctagaaa atctagctag aagtgctgga tttggagatg
     4801 taaatcgtat gatgtgggct cttgagactg aaaaactaaa tgaagttgct catctattta
     4861 aaccaaaaga tattgatggt acttcaattt ggatagatgt tgatgaaaac ggaaaagcat
     4921 ctactaaagt tcaaaaaggc aacaagtctt taaagagtat accaaaagct attgctaaac
     4981 atccatatgt acttgaactt aaatctgtcc aaaaagattt aaagagtcag tattctcgtg
     5041 caaaacagag tttagaacaa gctatgaaaa atgaaatagt atttacagtt aaagaaattg
     5101 ccaatatcta taaaaatcca gttatctgcc ctttgattaa aaatctagta tgggtatgtg
     5161 atgataatat tggatatgtt tataatgata agtctaagtt ctttatttgc aatatagaag
     5221 gtgaaactta taagttgacg gcaaaaaata aattaaaact tgcacatcca gttcatttgt
     5281 ttgaatctgg taagtggagt gagttccaac atgatttatt tagtaagcaa attgttcaac
     5341 catttaagca agtattccgt gagtattata gaataaacag tgatgaatta tctgaaaaaa
     5401 ctatatctcg cagatatgct ggtcaccaaa ttcagccaaa gaaaactgta gcacttctta
     5461 gaaatcagct ttggactgtc gattatgaag agggattaca gaaagtattt tataaagaaa
     5521 atatagtggc aaagatgttt gcacttgcag attggttttc accagcagat atagagcctc
     5581 ctactattga aacagtagaa ttttttgata gaaaaactta taaaagttta gaattagctt
     5641 ctatacctcc aattctattc tcagaagtta tgcgtgatat agaccttgtt gtaagtgtcg
     5701 ctcatgctgg tggtgttgac cctgaaacta gccattctac tgtagagatg cgtatagcaa
     5761 ttgctaaaga acttttatta ttattaaaaa ttaacaatgt caattttgaa ggctctcatg
     5821 ctcttatagc aggtaattta ggtgagtatt ctgtgcatat gggttctggt attgtacata
     5881 aaaaagggat gggtgctttg aatatacttc ctatacattc tcaaagtaga ggtaagatat
     5941 tccttccatt cgctgatgaa gaccctaaaa ctgctgagat tatgtctaag atactattac
     6001 ttgctgaaga tagtaagatt aaagacccta gtatcttaag taatttataa gtataaaaac
     6061 tatccctaaa atatattgga ctttcttaaa aaaaattaga aagtccaata tatactatac
     6121 tgtaatttat atagattaaa tcataattac aaataatttt tatataaact tctcttatac
     6181 attatcttta tggtacttat tatatcacac aaaaagtatt tttcttctat cttcactaga
     6241 atcttttaac cctataaaat ctatataaaa aatctttttc tagagatttg tttaatatgg
     6301 cattttcttg aaaatttata aaccttatct ctaatgaaga tttatttata ggtataacct
     6361 tatgtataaa tcttagattt actatgtatt gtctatgggt cataaagaaa ttaggaggta
     6421 ggcgtttttt taaatcatca aagttctcat aaaactttat tatatcttca tctttagtgt
     6481 gtagattaat tgctcttgaa tttttctcaa acataactat atcatcaaaa ttaatcatat
     6541 gtatctgctt ccttatttta tatacaaaaa gggtgtcttc tataaaatta ttattagcaa
     6601 tcttatggga gttaatatgg tctattgcta tgttaattga atcaagtatt ttttcttttt
     6661 taaatggttt aactacaaaa tctataggat gcactttaaa gctttcaaga ctataatcag
     6721 cgaaacccgt cacaaatact atatgtatat ccttatctat agaaagaatt tcttcagcag
     6781 aatcaatccc atttttatat ggcatatcaa tgtcaataat caataaatct attttctcat
     6841 tttttataaa ttcaattgcc tctataccat ccttaacttt tttaacataa tctacatatc
     6901 cagactcttt agctatattt gctatatcat ctcttacaat cttatcatca tcacaaatca
     6961 aaacttttat catagacttt tctcctccta actaataata ttttgggatt aagattacaa
     7021 attcagttaa aaaatcatca ctatttacaa gtatactata atcatactta tctaatatat
     7081 ttttaactaa atgtagccca tatcccctat cttgtccttc tttagtacta aatccattcc
     7141 aaaatatact ttcaacatta tgtattttag gaccattatt ttttatagct ataatatact
     7201 gaaattcatc ttcatatgca tgtactctta tatacttctt ttcttttttt atagtagaca
     7261 aagactctat tgcattatct attatgttta atagtactga actcatatca ttaggttcaa
     7321 tctctaaatt atccataaaa tcttgaatgt agacatcaaa agatatatcc ttttcaagac
     7381 attcctcgta ttttaatgaa gctatagcat ctaaataaga tacttttcca taccttgaat
     7441 tttttgattt aaaactattt gatatattca ttatatattc tttggcatct tcatctagcc
     7501 cagtatttaa tagagaatac accacttgca aatgtttcaa atagtcatgt ttttgacttc
     7561 ttaatttgat aattatatta tccttatgat ttattttact atctagtttt tgtagtttat
     7621 attcctgttc attattatta tttatcaatc ttataacttt aaagcctgta taaaaactta
     7681 aaataattaa aaccaaaaaa agtataatat ttaatactcc ttcaagtaaa aaactatatc
     7741 ctaaccttaa ttgtatatat ttagtgccct ccataaataa aatcaatgca aaacacgata
     7801 taaaaaatgt taatatgcct ctttttaata tgtctaattc tttgtaaaat ttatacttat
     7861 tcattataag attgctaaat ataaacaaag ttagtattac tataggaaat acaaaattgt
     7921 t
//
LOCUS       DAEMGG010000152         7895 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEMGG010000152 DAEMGG010000000
VERSION     DAEMGG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.153"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            398..1699
                     /locus_tag="KPK24_003333"
     CDS             398..1699
                     /locus_tag="KPK24_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2300973.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1775..2050
                     /locus_tag="KPK24_003334"
     CDS             1775..2050
                     /locus_tag="KPK24_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2300974.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3493..3723
                     /locus_tag="KPK24_003335"
     CDS             3493..3723
                     /locus_tag="KPK24_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2300975.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3716..4297
                     /locus_tag="KPK24_003336"
     CDS             3716..4297
                     /locus_tag="KPK24_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2300976.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSI"
     gene            complement(4872..5549)
                     /locus_tag="KPK24_003337"
     CDS             complement(4872..5549)
                     /locus_tag="KPK24_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2300977.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5738..7102)
                     /locus_tag="KPK24_003338"
     CDS             complement(5738..7102)
                     /locus_tag="KPK24_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2300978.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7230..7751
                     /locus_tag="KPK24_003339"
     CDS             7230..7751
                     /locus_tag="KPK24_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG2300979.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNNLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3088 a          883 c         1358 g         2566 t
ORIGIN      
        1 actatcaagt agtgaattat attgaacgat atattaataa ttggaagtaa gttatatcaa
       61 ataagatatt actatcaagt agtgaattat attgaacgat atattaataa ttggcagtaa
      121 gttatatcaa ataagatatt actatcaagt agtgaattat attgaacaat atattaataa
      181 ttggtagtaa agtatatcaa ataagatatt gctattatgt agtaaattat ataaaacaat
      241 gtatcagaat aaatagtata ttaataattt gtagtatatc atgacgaata atacagtcat
      301 aacaaatagt gagtcataac aaataatgta tttataattg ataatgtatt taaaaatcaa
      361 aatataaaaa cttataagga aagagagatt aatttatatg ggtaaatcaa aagatgttga
      421 aatagagaag tctattttag gcacaattct tttagacaat aaattaagct ataagctaga
      481 tgaattaaat gaaaacatgt ttatgaatga tatatgtctt gagatattta aaattatgaa
      541 agaacttaaa aaagaaaaca tagtgattga tgtggccact gtgaaatcaa agatagatag
      601 aaaatctcta gcaataaaaa cgagtgatgt gactaatcta attacttggg gtcagaattt
      661 tggtcttgat ggtcatatta aaattttaaa agaaaattta gctagaagaa gcataaatca
      721 aaattgccaa aaccttttac acagcctaaa ccttggagaa aatatcgata cttgtattta
      781 caaatttgaa agtaatataa aggagatttt ggacaaggat acttatgaaa atgatgatgt
      841 aaatagtata gcagggaagg tgcttgattt tttagaaaac aaaaaagata ttggatttaa
      901 atttggtata aaattacttg ataccactat aggaggtctt tttaaaggag agctaaccac
      961 aatagcagct aaatcggggg ttgggaagac tgctttggca cttcaaataa tgttaaattc
     1021 atttaagcaa ggtaaaaaaa ctttatttat aagtagagaa atgaccagtg agcaagtatt
     1081 tatgagaaat atatgcagag taactggtgt aagtacaagg aatatgaaat ctaaagaaat
     1141 agatgaaaat gattggaaac ttattgtaaa tgctataggt gatttaagcg aaaataactt
     1201 aatttatatc aatgataaga tagatacaat tagtgcaatt agaaagagga taaggcaggt
     1261 aaagccagat ttattaatag tggattatgt gcaactcctt acatctcaga aaagcatgga
     1321 taagagagaa agggaggtag ctactttttc gagagaactt aaaaatatga ctttagattt
     1381 taatatacct gtaatccaat taagtcagtt aaatgatgaa atgaaagatt caagacctta
     1441 tggagataga cctatgaggg atagcaaagc catctaccac gatagcaaca atgtggttta
     1501 tatacatcaa ttaaagggga gtgattatga agaagcagtg agagatattg gagaaagtga
     1561 ggaggcagtt agagcatcag aatacagggg aattaagatg gttgatttaa tagttgcgaa
     1621 gtgtagagat ggacagacta ggcataagca tttttgttac tttggggata agttacattt
     1681 tcaagaacta aattattagg tcaataaagt agtaataaca agcttattaa aagtcgggaa
     1741 atagcaaata gattataaaa tatgaggtga aaatatgttg gaattaagaa aaattataga
     1801 attacttgaa gaacacaaga tagaagtaag tattccaaag gagttacata agtatagttt
     1861 attggaatac cttgaattgt gtcaacaaag tgggggaaca tatttttata ttaaaacact
     1921 ggaaggggct ttaaaagagg ctagaaatga gcttatgatt aaattgttta atgggaaaaa
     1981 ttatatggaa ttagcgagga tgtttgaaat gagtgttatt aatgtcaggc atattgttag
     2041 aaaaaaatag atatttttgg agaatacaag tgtgatatac ttctgaaata gaagttatta
     2101 tagttgaaat aaaattgtgg ttaataaatt aaagttgtgt ataagtttaa gattatagag
     2161 gagtataagt gtgattaaca acatgatttt gtgaataaaa ttgaggatat atatgattta
     2221 aatgtgtgat taataaggta aggttgtgta taagattatg agtatttaaa aggaggggct
     2281 ataattaata gtgtagagtt gtgtgtttgc ttaagattat ataataattt aatagattaa
     2341 actatactaa taaggtaaaa tcgtgtgttt agttaaagct tattataaaa tcttgatatg
     2401 tgattaatga tgcaatatgt gaataaagtt aagattataa tagatttaac ctgtggctaa
     2461 tagaataaaa ttgtgaataa agttaagatt ataataggtt taacctgtga ttaatagagt
     2521 aagagtgtaa atagagataa acttcaatat gagcttaacc tatgattaat agagtaagag
     2581 tgtaaataga gataaacttc aatatgagct taacctatga ttaatatagt aagagtgtaa
     2641 atagagataa acttcaatat gggtttaact tgtgattaat agagtagggt gtaactaaag
     2701 ataaacttca atatgagttt aacttgtgat taatagagta aggtgcaact aaagataaac
     2761 ttcaatatga gcttaaccta tgattaatag agtaagaatg taaatagaga taaacttcaa
     2821 tatgggctta acttgtggtt aatagagtaa gagtgtaact aaagataaac ttcaatatgg
     2881 gtttaacttg tggttaatag agtaagaatg taaatagaga taaacttcaa tatgagctta
     2941 acttgtgatt aatagagtaa gagtgtaaat agagataaac ttcaatatgg gattaacttg
     3001 tgattaatag agtaagagtg taactaaaga taaacttcaa tatgagctta acctatgatt
     3061 aatagagtaa gaatgtaaat agagataaac ttcaatatgg gtttaacctg tggttaatag
     3121 agtagggtgt aactaaagat aaacttcaat atgagtttaa cttgtggcta atagaataag
     3181 attgtgaata aagttatagt tttagatggg tattaacctg ttactaatag aatatgatta
     3241 ggtgtaaaat tatagagctg tataagaaaa tattatagat atattaaata ttagataata
     3301 aatatttata tagatacaca aaagaaaagg cgatatgaag ctaaacttaa tgacttcata
     3361 tcgtttttta tttgaaactc ctttgtattt agagtgtgat ttttaatatt gaggttttat
     3421 gtgattaagt cacagataaa aagtaagtat cttgatataa ttcttataga ttaataatat
     3481 agggaggtta ttatgtctaa taaaaaaaag aagtatgcaa atattttagc aaatgagtgc
     3541 gttgcttgtg gtagctgtat aaaagcatgt ccaagaagtg ctatctcagt tccatgtggc
     3601 attagtgcaa aaattaatag agatttatgt gttggatgtg ggatttgtga aaagatatgt
     3661 ccagcatctg taattgagat tataacaata ttaaaagagg aagaaggtaa gtgtcatgaa
     3721 taataaaaaa cattggtatg actatttatg gattttttct tcaatatatt tgatattggg
     3781 atttgtaaat attattttcg cttggattgg gctaatttgt ttctttgttc cactagctat
     3841 ttctattgtt aagggaaata aagcttactg taataagtat tgtggaagag ggcaactgtt
     3901 taatttgctt ggaaataaat tgaagctgtc aagaaataga gatattccta aatttattag
     3961 aagtaagtgg tttagatatg ggtttttgac attttttatg gtaatgtttg ccaatatgct
     4021 tttttcaact tatttagtgt ttgaaggaag tagaaatttt aaagaagtag tgactttgtt
     4081 gtggactata aaaatgcctt ggcaatgggc gtatcatgga actttagttt caccttgggt
     4141 ggtacagttt gcatttggtt tttatagtgt tatgcttaca tcaactgttc ttggtttgat
     4201 tactatgata ttgtttaaac ctcgttcatg gtgtgtttat tgccctatgg gtactatgac
     4261 tcagatgata tgtaaggcaa agagtaagtc tatatagaaa ggctttatgt gatatttaca
     4321 gttttgttag agatgagtag taggtttgaa tatatatgac aaatatatga gtataggggc
     4381 atagatttag tttgtgtaag gtaaactgat ttatgcttct tttttatgtc ttagattatt
     4441 ggctaagtgg aaacaatgaa tttagatatt aagaattatt cagttatttg aagaaaatag
     4501 aataataatt aacattatat gaatggatat tcattacata taagccagtt aatagcaaga
     4561 tttatttaat atgaaatagg tcaagtattt aaactaaata aggtcatgac aattaatata
     4621 agtatagata taaaaagatt agaagttata acttataaaa ggtattctat ataaatagta
     4681 tgtggaaaat agtcaaatta acttattaga aacaaaaaat atacataaaa aaataaatgt
     4741 aaatatcaaa taagatacta tataaaaata gatataagta tcttttttat tattgaaatt
     4801 atgaaaatat atttgatttc acacaaaaaa gagtgcctca tgaactaaaa agttcatttt
     4861 gagacacact tttacagtgt caaagaacga agtttatcct tatccaatac ttttatacca
     4921 ccacgagata aagacacaat accttctcta gcaaaatatt tcaacattct cgatacaact
     4981 tctctggcac tacccatata cttagcaatt tgctcatgag tcatatttat agtgctactg
     5041 ccatttttag ctatctcatc aattaaaaaa gatgccaaac gcttatcaaa actcataaaa
     5101 agaatttgtt gcatagccca cataacatca gaaaaacgtt cggtagctaa tttatatgaa
     5161 aataactctg catgaatatt ttctgtagat aattttgcaa aaacagagga actaatctgt
     5221 ataatatcgc agtccgtttc agcatctaca tagacatcaa aagttattgt ttttagaata
     5281 caggaagctg ataaaataca tacatctcca tcatcaagtc ggaacagtgt aacctctcga
     5341 ccttcatctg aaagtatgta tgtacgtact gttccagact ttataatcaa aataccaata
     5401 cagtcactat caccacaatg tactggattc ccttttttgt aatgcgaggt atttgctgaa
     5461 gaaattaata attctttttg taagtcagta agtttatccc aaaaaggcaa gctatgtgta
     5521 agtatatcga tttcattttg tgaaatcata caatatctcc tttcttaaat tgttataatc
     5581 ccatgataca agtgaataga taaattgaca agtagtttgt taataggatt atttagtcag
     5641 atagtaatag tatgatttat gtagaaaaaa tatgataata tatattcttg ctattaatga
     5701 gaaggtaata tgttttgatg atagtaggtt tgtataacta atgcataccg tttattttta
     5761 aaagattaag ttccttatgt aaccttatag acataaaacc agcaattata gcagccatca
     5821 aatcagctat tggttgtgca tataagatac catttaaccc atagaataca ggaataatca
     5881 aaattatagg gataaaacag acaccttgtc tacacatacc taaaaatcca ccttcctttg
     5941 cttttcctag tgcgaggaat aatgtagaat acactgtata gaatccaaat agcataaatg
     6001 aaaacccatt tgccctaaga gctttactac cattttctat taatagaatg tcaccacttg
     6061 taaattgtga aattatagat tttgggaaga gtatcaatat aagaccaagc actacacaaa
     6121 atattgtagt ccataatatg gaagttttta ttgctatatg taatctatcg tagttctttg
     6181 aaccaaaatt ataaccagca attggttgga aaccttttat aaaaccaaat acaacaagac
     6241 tacctaaaga cgttattcgt gttacagcac ccattccagc tatcacagaa tcaccatact
     6301 ttgtagcctc catattagtt aatgtaatgg caagactagt caataattga aatattaagg
     6361 taggtatgcc aatttttagg atttctgaaa aaatttcttt tgaaaaacag cattcgttaa
     6421 ttctaaaact aaatatactt ttcttgctta aaatatatcc tacataaaca agtgttgaag
     6481 caatctgtga gatagctgtt gcaatagaag caccccttac ccccagacca aaagtataaa
     6541 tgaatattgg gtctaaaatc atattaagca aagcacctaa taacatagca cacatagttg
     6601 ttttagcagc tccttcactt gtgacaatat tagtcatagt gacattgaat atattaaaaa
     6661 tagatgacac gacataaatg ctagcataag atattgcata aggcataata ctttttgtag
     6721 ctcctaacaa ttttaaaatt ggattgagaa aaatcataat cacaataata actaatgtac
     6781 ccactataat actactgtaa atagatgtac ttaccacttt tcctgctttt tcattatcac
     6841 catggcctaa aagtctggca atatatgaag cagctccatt tccaaacaat aatcctaatc
     6901 ccacaacaag ctgacctaat ggaaatgcaa cggatatagc tcccatttga cttgttccaa
     6961 gtccacccac aaagtatgca tcaaccaaat tgtataatgc agttatcatc atacctatca
     7021 tggtaggtaa tcccatagat aacaatgctt ttgagattgg ggcagaccct aataattcaa
     7081 ttcttttgtt actttctttc atacttaaca ctcctcttct attgacatta agtctaaatt
     7141 ataatactat aaattatagt aagtatggaa aatagtacta taatttatag tatttgtcaa
     7201 tagtaagaaa actaaaggag atgattttta tggctacaat agatttaatt gtattgggaa
     7261 tagtaaagca acaacccaag agtgcttatg atattcaaaa ggcagtggaa tacaggaata
     7321 tatcacgttg ggttaaaata agtacaccat caatttataa aaaagtaatt caattagaac
     7381 aaaagggata tattgaaagt aaaactgtga agaatggaaa aatgccagaa aaagcagttt
     7441 attcattaac tgaattaggt gagacacatt ttttagattt aatgtttgat acatctagca
     7501 aaccaataaa tattctttta gattttaatg ctgttatagt aaatttaaat aaccttacac
     7561 cagaaaaaca gaaattatgt ctcaataata ttgaagagaa tgttcaagag ttaaagaaaa
     7621 ctattgagga aaatataagt ataaagacag aagtaccaga cacagcaaaa gcagtgttgg
     7681 agcaacagtt ggtgctagca caagcattgg aaacctggat aaattcttta aaagataata
     7741 tgggaaatta gaattagaga agctaactta ataatcatta aacaaaaacc agcatattta
     7801 ataatgagat attagaaaaa ataaaacaaa tgatataaac tgaaaagtat atttaaaata
     7861 aagctcactt aattgaggga gaatgttaaa ctttt
//
LOCUS       DAEMGG010000153         7612 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEMGG010000153 DAEMGG010000000
VERSION     DAEMGG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.154"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..547
                     /locus_tag="KPK24_003340"
     CDS             134..547
                     /locus_tag="KPK24_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG2300980.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDHIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            749..1390
                     /locus_tag="KPK24_003341"
     CDS             749..1390
                     /locus_tag="KPK24_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG2300981.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1414..2667
                     /locus_tag="KPK24_003342"
     CDS             1414..2667
                     /locus_tag="KPK24_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2300982.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            2713..3363
                     /locus_tag="KPK24_003343"
     CDS             2713..3363
                     /locus_tag="KPK24_003343"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG2300983.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3414..4010
                     /locus_tag="KPK24_003344"
     CDS             3414..4010
                     /locus_tag="KPK24_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG2300984.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4023..4862
                     /locus_tag="KPK24_003345"
     CDS             4023..4862
                     /locus_tag="KPK24_003345"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG2300985.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKKVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5107..6261
                     /locus_tag="KPK24_003346"
     CDS             5107..6261
                     /locus_tag="KPK24_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG2300986.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            6567..6743
                     /locus_tag="KPK24_003347"
                     /pseudo
     CDS             6567..6743
                     /locus_tag="KPK24_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
     gene            complement(6977..7126)
                     /locus_tag="KPK24_003348"
     CDS             complement(6977..7126)
                     /locus_tag="KPK24_003348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300987.1"
                     /translation="MQAQTISYPSALSVKVYPLPLPIACSVLPSRGIVVEVSPIRSLP
                     ADCPF"
     gene            7392..>7612
                     /locus_tag="KPK24_003349"
     CDS             7392..>7612
                     /locus_tag="KPK24_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2300988.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KKHKETFTYKQCSSDLTNLKKELKWLKEP"
BASE COUNT         3152 a          751 c         1333 g         2376 t
ORIGIN      
        1 ttatacagcc tctttattta ttgattgtat tattaataaa ttttaaatta tgataaaata
       61 taaaacaagt aacttatttc agaataaaat actttttaaa agaaagggac gagatatggg
      121 agattataat aaaatgcttg aattaaagaa taacttaggt atcagtgaaa gaaagttaaa
      181 atatccttgt atctataaac atttcaaagg gaaatattat gctacaatgg gtcttagcaa
      241 ggctattgat catatagaaa acatatgtga aatatatggt aaagaaaatt taatccaaaa
      301 cagaaataaa tataaattag ttattagaca tactgaaaga gaagaagata tctatgttta
      361 tagggattta gatggaaact tttatcataa aaaagaagaa gatactaatg atttagtttt
      421 atacaagacc ttatatgatg atacaggaat atttgcaaga cctttggata tgtttttaga
      481 aaaagtagat acagataagt atgtcaattc aattcaagaa tacagatttg aagaagtata
      541 taaataatat aagagaaata tgtttataaa tttagaaaaa ttctttaaca gtacaataat
      601 ttgcagaaat acaaatgaga tttattgtaa ataatatgtt agattagatt ataaaataga
      661 aacaaagtaa agtattatat gatataatta agttttgaga agtgataatt tatatttaat
      721 aaaaaatagg catatattag gaggacatat gggaaatact ttttatatag taagacatgg
      781 acaaacggat tggaatatat tgggaaaaac acaaggtcat ggtaattctg accttacgcc
      841 tcaaggtata gaacaggcaa aagaactttc tgaagatatt ggcaagtatt caattgatta
      901 tatttttagt agcgaccttg ggagagcaat gcaaacagct caaattttag gagataagct
      961 caatattgaa gttcaaaaaa ctgaggcact tagagaaatg ggattcggag tttgggaagg
     1021 ccttttaata aaagaaattc aaaaagatta ttcagatata tatgcaacat ggagaaatga
     1081 accacactta gtaaatatac cagaaggcga aactcttaag attataaagg aaagagtaga
     1141 tgcttttata aaggaactaa atgaaaaata tgataataaa aatataattc ttgttacaca
     1201 ttcaattaca ttgagggtta tgttgttgtc atttttagaa tcaggtatgg aaaatatata
     1261 tagaattaag caagataata ctgctttaaa tatagtagaa tttaaagatt atgggcctgt
     1321 tatagtaaaa atgaatgata caagtcatat aaaaaatcat gtaaagataa ataattctgc
     1381 actggagtag gaatcaatta taaggagaaa tttatgagta aagttatagt agttggtgga
     1441 ggagcttctg gaatgatggc agctttgtca gcttcaaaaa acaataatga agtaatatta
     1501 gttgagagaa atggagaatt aggaagaaaa cttagggcaa ctggtggcgg tagatgcaat
     1561 tttaccaata atagagagat agaagatttt tttgataaag ttgtcagtaa taaaaaattt
     1621 ctctatagta gtttctatac atttacaaat aaagacctta tttcgtattt tgaaagtaga
     1681 aatttggaat ataagattga agaagaaaat gaccacaaag tatacactaa aaatgataaa
     1741 tctatagaag taatagaagt attaaataaa gatttactca atcacaatgt aaagataatg
     1801 tataataaga aagttataga tattataact gaagaaattg ctctaaaaga tgattctaat
     1861 aaggacaagt caaagtatct aataaaaggg attattttag ataatggaga taaaatacta
     1921 ggagacaagg ttattatatc aactggagga gtgagttatt caaagacggg ttcagatggt
     1981 tcaatgtata agattcttaa aaagcatgga catacattaa acaaactata tcctgcttta
     2041 gttccactta caatagagga aaaatggatt aaagatttac aaggaatatc catgaagaat
     2101 gtggaaatat cttgtaagat taagaaaagg aaaatatcaa aatctggaga catgctattt
     2161 gcccattttg ggataacagg accttgtgtt cttataatgt cttcttatat taacaagatt
     2221 attgaaaagg aaaaggtaga attaaatata gatttcttac caaatttaag tactgatgaa
     2281 atctctagta taatcagagc atttccaaat aaaaatgttc taaataatct taagcaaata
     2341 ctacctcaaa atttcttaaa agagattttt tctctattat ctttagttga taaaaaggca
     2401 agtgatttgt ctaaggcaga tgaaattaga attattgagt atataaaaaa tatgaagcta
     2461 acttgtaatg ggactacagg tataaacact ggtatggtta cttctggtgg tatttcagtt
     2521 aaagaaatta attcttctac tatggaatct aaattagtta agaacttatt ttttacaggt
     2581 gaagtaattg atatagatgc agaaactggt ggatataatt tacaaatagc attttcaact
     2641 ggatatcttg cgggtataag tgtataaata taatttttta ataattaaat atgaataaat
     2701 agaggtgtat atatgggtaa tttagttata gctgtagatg gtccagcagg agctggaaaa
     2761 agtacaatag ccaagatagt agcaaaaaag ctaaatataa attatataga tacaggtgct
     2821 atgtacagag cagttactta taagtgttta aaaagtggta ttgatgtcaa taatgaaaaa
     2881 gaagttattc aaatagctga aaattcggat atagatttta aagataacaa tatatatttg
     2941 gacaaagaag ttataaatga agaaataaga acaatagaag taagcaataa tgtttctaat
     3001 gtagcaaaaa taaaagaagt aagacagttg atggttgaag ttcaacgaaa aataggaatg
     3061 aaaaattcag taatactaga tggtagagat ataggttcat atgttttccc agatgcagat
     3121 tacaaattct ttttagtagc tactccagaa gaaagaggaa atagacgcta taaagaactt
     3181 tgtaataaag gatataatac tactttagaa gaagtaattg aggatataat aagacgagat
     3241 gaaattgatt caaatagaga atttgcacca ttggtaaaag ctaatgatgc actagaaata
     3301 gatactacag gaaaaactat agaagaagtt gtagaagaag ttgtatctaa aataaatctt
     3361 tagtttttta tgcatcataa tgaaaataaa tgtagtgttg aagggagata aacgtgaatt
     3421 tttatagatt tgtaataaat atttttaagg gtttttctaa aatatttttt aaatatgaag
     3481 ttataggtgc agagaatatt ccagatagag gaaatatagt aatagcatca aatcataaat
     3541 cgaatcttga ccctatattc ttggctgctg ccatagaaaa tagagaaata gctgcaattg
     3601 caaagaaaga attgtttaaa gtaaaaccat tgggatttat attaaaaaaa ttgcatgtta
     3661 tgcctataaa tagagaaaaa ccagatgtat ctacaataaa aactatttta aggtcagtta
     3721 gagacggtta tgtattagga atatttccag agggtactag aataaaagga gattcttttg
     3781 gtaaggctaa ggcaggtctt tctgtattta caataaaaag taaatctaaa gttgttccag
     3841 tatcaataat atctaaatat aaacttttta gtaaagtaat tgtatatatt ggagaaccaa
     3901 tatcatttga agaacatttt aaggaaaaat tgagtaatga tgaccatgaa aggatatcac
     3961 aagagatttt agaagtgata aaacaaaatt actttaaata ttcaaaataa gggggtaaag
     4021 ttatgaacat aaaaatagca aaaaatgctg gcttttgttt tggagtaaaa agagcaatga
     4081 aaatggcatg ggatgaagtt gaaaaaaatg attctggcat atatgcttta ggtcctctta
     4141 ttcataacaa gcaagcagta gcaaaatatg aagaaaaagg tcttaagact gtgaatgaaa
     4201 ttgatacaat accaaatcat gaaaatatga taataaggtc tcatggagtt cctgaaaata
     4261 tatataagga agccaaagat aaaaaattaa aaattgtgga tactacatgt ccatttgtaa
     4321 agaaaataca tacagtagta agtgaatatc ataataaagg ctatgaaata atagttattg
     4381 gagatatgaa gcatccagaa gttataggta taaatggatg gtgtgaaaat tcagctataa
     4441 taataaagac cttagaacaa atggaaaata tggaatttga taactccaaa agatattgtc
     4501 ttgttgcaca gactacaata aatccagagt tgtatataag tatagttaat aaactatcag
     4561 ataaattaga agaaatagta tttaatgata caatttgttc agctacaaaa acaagacaag
     4621 aatcagctaa agagttagct aaaaaagttg attgtatgat agttatagga ggtaaacaca
     4681 gttcaaatac tcaaaagtta gtaaaggttt gtgaagactt agtaccaact tttgcaatag
     4741 agactaaaga tgaattggat gtaaatacgc ttaaaaaata taaaaatttg ggtattacag
     4801 caggggcatc tacacctaat tggataatag aagaagttgt aacattcctt gaaaatttat
     4861 aatttgatag gtaataaaaa taggtggaca ttgttataat ttatcataat atccaccttt
     4921 gatttcatat tacatgtggt aaatagataa cctaatatca aaaagtgtgt atcttaagat
     4981 gaaaaactaa gagtataaga tgcgcacttt ttaatattag aaattcaaaa gaaatattga
     5041 tattgattat caatagaaaa tagaatataa tataattgtt gcaaaattag tctaggaggt
     5101 aaattaatgg aaatgttgat atccgcttct gaagaggcgt ttttacatgt aggttctatg
     5161 attggatttt ttattttgtt atttggatat attaattata aaactagtgg aaattttact
     5221 aatatcatat caaaaaacag aaaatttcaa cccttaatag gtgctttaat tggagctata
     5281 ccaggttgtg gcggaagttt ggcaataatg ccattgtata taaatggaaa attaagcttt
     5341 ggagcaataa ttgcaagttt aatagctagt atgggagatg cagcatttgt acttatttca
     5401 tcaaatatta aaatgtattt ttttgtcact atagttagta caattacagg aataataact
     5461 ggacaattag tggattactt taaactagaa gaaaaattag ggttaaaaaa ccgtagaaaa
     5521 tcagataaat atgatataag taaaaataac cctaaagaaa ataaagaaca acataatcat
     5581 gatgaaataa ttttagacac tctagctaaa tctcatggaa atacaaatag acttgcattt
     5641 ataataacac atggaaaggg atataaaatt tatataggaa ttatagtaat cggttttata
     5701 tttatgtcac ttgctcactc tggacttaat cttcctataa ttgagaagtt acattcattg
     5761 gaagaagtaa tagcagtgat tggaatatta ttttcaataa tttatatgtg gtgttttaaa
     5821 aaggtattta aaaattctaa tcaaaaagag gaagaaaata aaaagatttc tttgcgagaa
     5881 atgcttatcc attctgttgg agaaatatca tttgtaatta catggatttt tattgcatat
     5941 cttatttacg atttgataat attaatttta ggtggagatg aatacttagt taaattagta
     6001 ttatcaacag gtgtaataag tgtatttata ggtgcaggtc ttggacttat tccaggttgt
     6061 gggatacaaa ttgtgcttat gtcattttat ttgaagggaa ctataccact tggtgcagtg
     6121 atagccaact ctatttctca agatggagat gcacttttcc cacttttagc aatggataaa
     6181 aaatcttctt tatgggctat gattattact actataccag caatacttgt aggtatgata
     6241 gtttatgcat ttttaggtta aatataatta tgaataaaga agtattaaaa aaataataaa
     6301 gcacataaat ttatttataa gaatagtgaa gattaaatat aaaaataaaa aattcaatat
     6361 gaattaaatt attgcttaaa tattaatgag aacatgggag ataatattat tcctcatgtt
     6421 ctctctattt aattaaagga attatggaac aaatttcaag tgatactttc tatcaaaact
     6481 catatataat atatattaat aaattaaact ataatacgta atacagtcag tatattgata
     6541 taaaaaactt aggaggagtt gtaaaaatgg gtaatttaaa aagacagtgt gatgtttcaa
     6601 gtggaagaga aaaagctgac attgtactaa aaaatgggac aatcataaat gtatttacag
     6661 aagagttaat aacaggagat gtagcaatag taggagacac tatcgttgga attggcgatt
     6721 acaagggcaa tgtagaaatt gattaaaaaa tagctacgca cactcgtgac tttagtcatg
     6781 agttagtagc taaaatagtc agcatatagg gaaacctata tgtagagata ggatagaacc
     6841 tatccaatac tacttgaatt gctggaaacc cctaaagcta atcaaactaa aacataagga
     6901 tgaaataaac ctaagtgtga aagttacgaa agtagaaaaa attggttaga tggtataagg
     6961 ttaaacccta agtactttaa aatgggcaat cagcaggtaa actccgaata ggagaaactt
     7021 caacgactat tcctcttgag ggaagtacac tacaagctat tggtagtgga agtgggtaga
     7081 ccttaacgga taatgctgaa ggataagata tagtctgtgc ttgcatgaaa gtgtaagaag
     7141 ttcataagag aactgcatag gtagtagcga acttatgtga acgacaacct caaaaatgat
     7201 aaaagttaag acttacggtt cttaaatatg ttatttaaaa ttaaatataa aaccttctaa
     7261 ataattgttt taaataatta catttgttaa cattattata ggtgattaat aaattgttca
     7321 ccacagcatt atataatata ttaaatatga taaaatcatt aaaaagtgta tcaaaaaaca
     7381 attaaagtga ggtgatagta gtggaaaaag cttataagtt tagaatgtat ccaaataaaa
     7441 aacagcaaga gttaattaac aaaacttttg gatgttgtag atttgtgtat aataagtatc
     7501 ttgctaaaag aatagatgtt tataaaaaac ataaagaaac ttttacttat aaacagtgta
     7561 gttctgattt gaccaattta aaaaaagaat taaagtggct taaagaacct ga
//
LOCUS       DAEMGG010000154         7552 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEMGG010000154 DAEMGG010000000
VERSION     DAEMGG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.155"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1048
                     /locus_tag="KPK24_003350"
     CDS             <1..1048
                     /locus_tag="KPK24_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBG2300989.1"
                     /translation="WISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTLHKEE
                     ISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTEFRDK
                     VLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGIIFSMC
                     TKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMKRSNY
                     PTFSDYLVEKFFDQIAHYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNSTLVA
                     LDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFFGVTI
                     GAIWASVIIKRMIR"
     gene            1152..2336
                     /locus_tag="KPK24_003351"
     CDS             1152..2336
                     /locus_tag="KPK24_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2300990.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGTKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2543..3256
                     /gene="rpsB"
                     /locus_tag="KPK24_003352"
     CDS             2543..3256
                     /gene="rpsB"
                     /locus_tag="KPK24_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG2300991.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3335..4246
                     /locus_tag="KPK24_003353"
     CDS             3335..4246
                     /locus_tag="KPK24_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG2300992.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4331..5035
                     /locus_tag="KPK24_003354"
     CDS             4331..5035
                     /locus_tag="KPK24_003354"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG2300993.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5061..5618
                     /gene="frr"
                     /locus_tag="KPK24_003355"
     CDS             5061..5618
                     /gene="frr"
                     /locus_tag="KPK24_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG2300994.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5621..5824
                     /locus_tag="KPK24_003356"
     CDS             5621..5824
                     /locus_tag="KPK24_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2300995.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5853..6587
                     /locus_tag="KPK24_003357"
     CDS             5853..6587
                     /locus_tag="KPK24_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG2300996.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6600..7379
                     /locus_tag="KPK24_003358"
     CDS             6600..7379
                     /locus_tag="KPK24_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG2300997.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3112 a          781 c         1443 g         2216 t
ORIGIN      
        1 atggatatct aaaatagatg agagaaaaaa atgtcataga gaagcagatt cttctggata
       61 ttgtatcttt cataaagaaa ataagtctga tgaagagata caattgatga tggatactct
      121 tcataaagaa gagataagtg aatttaatgg atttgttttt gaaaatgaat ttaatgcgga
      181 agaaatatta acttacaatt ataaaatact ggatttttct gaatccatat ttaaacaaaa
      241 agcaaatttt aaaaaatata tttttaaaaa gaatattatt tttaactata cagaatttag
      301 agataaagtt ttatttaatg gttgtgtatt cttagaaaac tgtgatttta atagaaccat
      361 atttagtaaa cactatataa atgaccgtat atttgaaaaa gtgaaattta aaggacctga
      421 tttggtagtt aataaagtag aaaattttcc acgaatggat ggaataattt ttagcatgtg
      481 tacaaaattt gtgttaaaga atgttgaata tggaaaaagt gagtatgagc atggcaagat
      541 aaattataga atagctagaa atcaagcaac aaaaattgga gaatatgaga tgataggatt
      601 ttactattat aaggaaagaa tttatagtag taaaataatg aaacgttcta attatcctac
      661 cttcagtgat tatttagttg aaaaattttt tgaccaaata gcacattaca caacaggtta
      721 tggagaaaag ccatggaata tattattagt tattatagca ataataagtg tttttgcttt
      781 attatactta tttgtaggca tagaaagttc aaatagcaca cttgtagctt tagatataaa
      841 taatataggt gattactcac taagtgagat tttcaaaatg tatatggatt tatggtattt
      901 tagcatggct acatttagta cagtaggata tggagatatg gttgctacaa gtttgattgg
      961 gaaagcattg gcaggtatag aagtattttt tggagttaca ataggagcaa tttgggcatc
     1021 tgtgattatt aaaagaatga taaggtaata tttgtgtaaa tatggatagg tattaattac
     1081 tagatataaa ttggactaaa tagggaaaat ttaaatataa atatacttaa taatccaaga
     1141 aggtgaaaaa tttgaaaaat gcactaaaaa aaattatatc attagccatg attctagttt
     1201 ttataatacc aaattattca tatgcagatg ataaatctgt agacaagtat tcaaaaacat
     1261 ctatacttat agaccaagaa acaggtagag ttttatatag taaagaacct gatatgaagg
     1321 taccattagc tagtttaagt aagatgatga cttttttact tgcaatagaa gctattgaaa
     1381 ataaagaagt aaaagaaact gataaggtaa aaatagacaa atctattgca tcagtaaaag
     1441 ggtcaagtta taaacttaaa gatggtgaag aagtgccact tatagagctt atgagaggtc
     1501 ttatgattgt atcaggaaat gatgcggcaa ttgctatagc aaaacatgtt tgtaaaacga
     1561 aagaagcatt tgtagataga atgaataaaa aagcaaaaga aataggaatg agtaatacac
     1621 attttctaaa tccaaatggt cttccaatat acgatttaag caaccccaaa aaaccagcaa
     1681 aagaaaatat ctctactgca aaagatatag ctattcttgg aaaatacatg tttgaccatt
     1741 atgaaaaaca agtaacagct ataactgata tggagacata tacaaattca gatagaagtt
     1801 ttgaaaaaag caatacaaat gcattattaa gaattatacc agaagtagat ggtataaaaa
     1861 ctggatatac tggcaatgct ggatattgct tatcattttc tatggttgta aataaaaatg
     1921 ataaaaatga gaaaaaacgt agagtaatag gggttgtatt aggtactaac cataaaaata
     1981 aaagaacctc tgcttcatta gctctactga aatatggaaa agaaaatttc aatatgaaaa
     2041 aagtaattga taaagatagc tttattggta agaaatatat aaaaggaacg aaagagttag
     2101 aggttacatt aaaagcaaaa gatgaacttt atacaatatt aaaagatgat gaaagtataa
     2161 agtcagaagt taagattaaa aatatagagt accctgttaa aaaaggtgat actatgggta
     2221 ttataaaata ttatactaat tcaggtgact tacttggaag tgtagatata gtaagtaata
     2281 atagtataga taacgtatca ttaaaaacaa aattaaaaat gaaatttgca aattaaattc
     2341 aaaaaatggt ataaaataca caaaaaaata taaaaataga aaatatggaa aaaatattgt
     2401 tatttcttca cctctgtgat atactactag aggtgaaaaa ataaaataaa tcacacacag
     2461 cattgggatc cttaaaaggt gcctattaca tggtttttta gggagaagat caagctggag
     2521 gtataaaacc aggaggtaag atatgtcagt aatatcaatg aaacaattat tagaagcagg
     2581 tgttcacttt ggtcaccaaa caagaagatg gaaccctaaa atggctaagt atatattcac
     2641 agagagaaat ggaatatata taatcgactt acaaaaaaca gttaaaaaag ttgaagaagc
     2701 ttacaagttc actaaagaag tagctgaaac aggaaagcca atcttattcg taggaactaa
     2761 aaaacaagct caagatgcga taaaagatga agctgaaaga tgtggaatgt actttgttaa
     2821 tgaaagatgg ttaggtggaa tgttaacaaa ccacaaaact ataaaaacaa gaataaataa
     2881 attaagagaa ttagaaaaaa tggaagaaga aggcgtattt aatgttcttc ctaaaaaaga
     2941 agttataaaa ttaagagctg aaaaagaaaa actagaaaaa tacttaggtg gaataaaaga
     3001 catgcctgaa ttaccaggtg caatgttcgt agttgaccca agaaaagaaa acatagctat
     3061 tcaagaagct catagattag gtataccagt agttggtata gttgatacta actgtgaccc
     3121 agaacaatta gactttgcta taccaggaaa tgatgacgct ataagagccg taaaattaat
     3181 aactggtgct atggcaacag ctgtaattga aggtagacaa ggtgctgagg aagaagtagc
     3241 tgaagaccaa gaataataat ttgacaatta aatggtaagg gtcgccctta ccatttataa
     3301 tatggaatag aataagtagg aggaaactta aaaaatggct aatataactg ctcaaatggt
     3361 aaaagagtta agagaaagta caggcgcagg aatgatggac tgcaagaaag ctttacaaga
     3421 agctgaagga aacatggaaa aagcagtaga tttattaaga gaaaaaggat tatcaaaagc
     3481 tgctaaaaaa gctggtagag ttgctgctga aggtctagtt gcaatagaaa tgaatgatga
     3541 taatacagtt gcttctatgg tagaagttaa ctcagagaca gatttcgttg ctaaaaacga
     3601 agacttcaaa gtgttcgtta aagatgctgc ttgtatggca ttagctactg ataaagaaga
     3661 tatagcttct ttattaggtg aaactcataa agagggaata actttacaag aagttttaaa
     3721 taatagagtt gcaaaaatag gtgagaaatt agactttaga agatttgcta aagttgtaac
     3781 taatggacaa gttgctggat acattcacgg tggtggaaaa ataggtgttc ttgttgaaat
     3841 ggaaacagaa gctagagatg ctaaagtttt agaattaggt aaagatgtag ctatgcaagt
     3901 tgctgctatg aatcctaagt atgtttcaag agatgaagta gatgctgaat acatagcaca
     3961 tgaaacagaa gtattaactc aacaagcttt aaatgaagga aaaccagcta atatagttga
     4021 aaaaatggtt aaaggaagat tagaaaaaga attaaaagag gtttgtttat tagagcaaac
     4081 tttcgttaaa aatccagata taactgttaa acaattagtt gctgatgttg ctaaggcagt
     4141 aggttctgat ataaaagttg ttaaagttgt gagattcgaa gttggtgaag gtatacaaaa
     4201 gagagaagaa aactttgcag aagaagttgc taagcaactt aagtaataac caaagagaga
     4261 acacgcaggt gttctctttt ttaaaaaata gaaaaaattg gatatgaagg attgaaatag
     4321 gaggtttctg atggataaac caatgtacaa aagggtattg ttaaaattaa gtggagaagc
     4381 attagcagga gaaagaggtt ttggtataaa taatgatgta gttaatgata ttgctattgc
     4441 tataaaaaaa atacaagaaa taggtgttga agtagctgta gtagtaggtg gaggaaactt
     4501 ctggagaggc agaaccagtg aaggaatgga cagaactact gctgactata taggaatgtt
     4561 agctacagtt atgaatgcaa tggcgctaca ggatgcacta gaaaatatag atgttgctac
     4621 aagagttcaa actgctatag acatgagaca aattgcagaa ccatatataa gaagaagagc
     4681 agttagacac ttagaaaagg aaagagttgt tatatttggt gcaggaacag gaaaccctta
     4741 ctttacaaca gatacaactg ctgctttacg tgctgctgag atggaagcag aagttatatt
     4801 gttagctaaa aatgttgatg cagtatatga caaggatcca aaagtacatg cagatgctaa
     4861 gaaatttaca gaattaagtt acatggaagt tatacagaaa gaattaaaag taatggattc
     4921 tacagctaca tctttatgta tggataataa aattccaata aaggtatttg agcttacaac
     4981 agagaatata ataagagcag taaaaggcga aaatatagga actactgtaa aataatgaag
     5041 aatgaaggag gagtaaaatt atgaaactgg aaatacataa gcaattagaa gagaaaatga
     5101 atgggacaat tgatgcttta aagtttgaat ttggaactat aagagcagga agagctaatg
     5161 cacaaatgct agacaaaata agagttgatt attatggaac tccaactcca ataaatcaaa
     5221 taggtgctat atctgtacca gaaccaagaa tattaatgat atctccttgg gataaatctg
     5281 ctatgcatga aatagaaaaa gcgatagcta actcagactt aggattaaat ccatcaaatg
     5341 atggtgaggt tataagactt tctgtacctg ctttaacaga agaaagaaga aaagaattag
     5401 ctaaaaaagc tagtaaagct gcagaagaat ttaaagttag aataagaaat gaaagaagag
     5461 acgccaatga gaaaattaaa aagatggaaa aaggcggaga attaactgaa gatgaattaa
     5521 aaaaagcaca agatgaagtt caaaaaatga ctgataaatt tataaaagaa atagatacat
     5581 tattatctaa aaaagaaaag gacataatgg aggtataata ttgaaaaaat attataacct
     5641 tttaggtctt catgtagatg aggtaaaaca atattttgat gagcaaaata taaattatac
     5701 cgtaaaatct attgaaggta aaaaggataa agaaaaactt gtagttccta gagttataaa
     5761 aatatctgag ataggtgata gtgtagaact gataatcaca tacttttctg actccttagt
     5821 ttaaggagtc agaacaatat tggaggcaaa atatgaataa caacattata tatgatatag
     5881 acttaaataa catacctact catattgcta ttataatgga tggaaatgga agatgggcaa
     5941 aagctagatt tcttccaaga actgcaggac ataaggctgg agtagaaact ataagagata
     6001 tagtaaaaga atgctcaaaa ttaggtgtta agcacttaac attatatgca ttttcaacag
     6061 aaaactggaa aagaccgaaa cttgaagttg acacacttat gaacttatta tctacatact
     6121 taagaaatga aatagctgaa cttcaccaaa acaatgtaaa agtaactgct ataggagata
     6181 taagtgcttt accaaaaact tgtataagag aattaaactc agctaaggaa ctgacaaaaa
     6241 acaatacagg agttaactta aatttagcat taaactacgg aagtagagca gatataaaaa
     6301 atgctcttat agatatagta aaaaattgtg aaagtggtaa aatagatata aacaatattg
     6361 atgaagatat tataaaaaac tatttaagca caaagtctat accagatcca gacttggtaa
     6421 taagaacaag tggtgaacaa agattgagta actttttact ttgggaagtt gcttattctg
     6481 agttttactt tactgatata cattggcctg attttaaaaa agaagaacta cagaaggcta
     6541 tatacgtata tcaaagaagg gatagaagat ttggaggact taagtaagga gattatttta
     6601 tgcttacaag aattattgct tcactagctc tggttccatt atttctattt gttgtatatg
     6661 gaggtattcc tctatatata gctgagacag caatagttta tattgcattg catgagtttt
     6721 ataaggcatt taaaataaaa gatgtacatc caatattcat tataggatat ttattttcta
     6781 tttatttagc tgtaaaaaat atttttaatt taccattaga atatacttat gcagtaattt
     6841 ttattttatt tttagcaagt ataatttata tgttgatggg caaaaacaat gttatagatg
     6901 tttcgattac gtttttagga gttttttata taggagtatt tttagatttt ataattataa
     6961 ctataaatgg atttgaaaaa ggtagtatat atgtatggct tatatttgtg atatctttta
     7021 tgacagatat atttgcatat tttagtggtt atttacttgg aaaacataag ctaattccta
     7081 aagttagtcc taaaaagact atagaaggag ctattggagg aataatagga agtacacttt
     7141 gttgtatttt atttggatat ttatttggca ttgatttgct tcaattagcg ataataggaa
     7201 gtataggaag tgtaatagct caattaggag atttatttgc atcttctata aagagatatg
     7261 tagggataaa ggattatgga aagataatac ctggtcatgg gggtatatta gatagatttg
     7321 atagtgttat attagttgca ccttttgtat atagtgcgat taaatttttt attagataaa
     7381 gattggttgg atttaaaaga gcaatctata attgctagta atgaaaatag ctatgtaact
     7441 tttaaatgca gagatattct gcagttatta gttacatggc tattttcatt gttcaaaaat
     7501 ttagaagacc ttggggaatt gtataagaat aggtattact ttataatagt at
//
LOCUS       DAEMGG010000155         7469 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEMGG010000155 DAEMGG010000000
VERSION     DAEMGG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.156"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            124..633
                     /locus_tag="KPK24_003359"
     CDS             124..633
                     /locus_tag="KPK24_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG2300998.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            645..1526
                     /locus_tag="KPK24_003360"
     CDS             645..1526
                     /locus_tag="KPK24_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG2300999.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     misc_binding    1654..1859
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1985..3256
                     /gene="serS"
                     /locus_tag="KPK24_003361"
     CDS             1985..3256
                     /gene="serS"
                     /locus_tag="KPK24_003361"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG2301000.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     gene            3577..4032
                     /locus_tag="KPK24_003362"
     CDS             3577..4032
                     /locus_tag="KPK24_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG2301001.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            4033..4124
                     /locus_tag="KPK24_003363"
     tRNA            4033..4124
                     /locus_tag="KPK24_003363"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4067..4069,aa:Ser,seq:gga)
     gene            4162..4426
                     /gene="ffs"
                     /locus_tag="KPK24_003364"
     ncRNA           4162..4426
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KPK24_003364"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4503..6140
                     /gene="dnaX"
                     /locus_tag="KPK24_003365"
     CDS             4503..6140
                     /gene="dnaX"
                     /locus_tag="KPK24_003365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG2301002.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            6199..6549
                     /locus_tag="KPK24_003366"
     CDS             6199..6549
                     /locus_tag="KPK24_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG2301003.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            6562..7161
                     /gene="recR"
                     /locus_tag="KPK24_003367"
     CDS             6562..7161
                     /gene="recR"
                     /locus_tag="KPK24_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG2301004.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            7302..>7469
                     /locus_tag="KPK24_003368"
     CDS             7302..>7469
                     /locus_tag="KPK24_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301005.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRN"
BASE COUNT         3034 a          751 c         1386 g         2298 t
ORIGIN      
        1 tcttttttat ttattataaa agttaagatt aaatagaata taaagtcaat attgatttat
       61 aataagataa aatataaaat tatatagtat aggtaaagtt tttatttgga aagaaggtgt
      121 aatatgaatt ctaatgaaag aagaagtaaa ttaatagata ttttaaaaga atctaagcat
      181 cctgtaaaag gtggaacact agctgaacta ttaaatgtaa gtaggcaagt aatagttcaa
      241 gatatagccc ttataagagc tagaggattt gaaataatag ctacacctca aggatatata
      301 atatataatc aaccgtattt tacaaaacaa ataaagtgta aaaatcataa ggattctaaa
      361 gaaatttatg atgaattaaa gataattgta gatttaggtg gtattataaa agatgttatt
      421 gtaaatcatc caacatatgg tcagataact gcagaactta atatttattc taaaatggat
      481 atagacaact ttatgaagaa agttgagaca aatgaattta aacaattatc ggttctgaca
      541 gaatgtagcc atattcatac aattgaggca ataaaagaag aaactataga agctatagta
      601 aaagagttaa aggacaacgg aatattatca taaggaggag ttgaatgaat tttttatcat
      661 tggctaagac tgcctcaaca aatagcaata taactaagag aactttagaa aatatgctcg
      721 ataattttag tgataatata ccaaatatta tatatgcaat aattgtattt gtgataggta
      781 tttatatatc taaaatagta agaaggatgg tttcaaagtt tctaatcaaa tatggaatga
      841 gtaaaggtgt aaataatttt attgtatatg gaatatatat atctatgtta tctattatat
      901 cattgataag cttaggtatt atagggatac aaactacatc agttgtagct gtgttaggag
      961 ctgctggatt tagtataggt ttagctttta aagaaatatt atcaaattta ggctctggta
     1021 tgattatttt attttttaag ccatttaata taggtgatta tattcaaggt tcaggtgtag
     1081 aaggtacagt ttctgacata caaatattta gcactgttct taagacgcca gataataaaa
     1141 ctataattat acctaatttt caactgacaa gtaataatat aattaattac acacatcaaa
     1201 acaaaagaag aatagatttt tcatataata tttcatatga tagtgatata gatgtagtta
     1261 agtctatatt aaatgaaatt tttacaaacg aaaaaagaat attaaatgat ccaaagccta
     1321 taataggtct aaactctata ggaaataaca ccatgcaaat agttgctaga ccttgggtaa
     1381 aaactgatga ttactgggat gtatactttg atgtaatgga aaaggtaaaa aataaatttg
     1441 atgagaatga tataaaagtt ccatttgttc cttcttcatt attatttaat aatacaggta
     1501 atctaaatac aatggataaa agataaggtc ttaattaaat agaaaaaatg aatttaatat
     1561 aaaaaattaa agaaaattct aaaaaaattt gaataatagt attgaaagat gttaatatat
     1621 ataatataat gattatcaaa taaaacaatt agttaaaaag ctgtgaagga aaatagtaaa
     1681 aaacagattg tattcagaga atgagtggat ggtgaaaact cattacaatg gtttttgaat
     1741 ctactctaga gcttctagtt aaagctagac gtttacctac gttataggtc tgagaggatt
     1801 atacatttta gtatagtcaa ttagggtggc aacgcggata aaagattttc gtccctttta
     1861 tagggtaaac ggaggtcttt ttttattttt aaaatttgtt taaactaaga gtttacaagt
     1921 ataatgtttg taaattaagt tggaattata acttatatta tcaaaattat taggaggaat
     1981 taatatgtta gatattaaaa gaataagaga aaatctagat gatatcaaaa aagctatgga
     2041 aagaagaggt gaaagagaat ttgaccttga tgctgtagtg gaattagata ataaaagaag
     2101 agaaatactt caagaagttg aagttatgaa aaatgagtta aatgtaaatt ctaaaaaaat
     2161 acctcaatta ataaaagaag gaaaagatgt aacagaagaa aaggcaagat tgaaagagtt
     2221 atcagataag ataaaaggta ttgatgagaa agtaaaagaa gtagaagcaa aaatggaata
     2281 tacattaatg agaataccaa atgttcctca tccagaggta ccacaaggtg aaacagatga
     2341 agacaatgtt caaataagaa cttggggaga acctactaaa tttgattttg agcataaagc
     2401 gcattgggat ataggtacag gtctaggtat tttagatttt gaaagagcag ggaaaataac
     2461 tgggtcaagg tttactttat ataggggatt aggagctaga ttagaaagat ctttaatgaa
     2521 tttcttttta aatactcata cagctaagca tggctatacg gaagtcttac ctccatttat
     2581 ggccaataga aatagtttta tagggactgg tcaacttcct aaatttgaag aagatatgtt
     2641 taaaatagaa ggattagagt atttcttaat accaacagct gaagttcctg ttactaatat
     2701 acatgctaat gaaatattag atgttgcaga gcttccaatt aaatactgtg catatactcc
     2761 ttgttttaga tctgaagcag gttcagcagg aagagataca agaggtttag ttagacaaca
     2821 tcaatttaat aaagttgaat tagttaaatt tgttaaacca gaagattctt ataatgaatt
     2881 agaatcatta actcatgatg ctgaaactat gttacaaatg ttaggtcttc catatagagt
     2941 tgtaaaaata tgtacaggag atttgggatt tacagctgca tttaaatatg atttagaagt
     3001 ttggatgcca agctacaata gatatgtaga aatatcttct tgttcaaatt ttgaagattt
     3061 ccaagcaaga agagcaggaa taagatttaa aagggataaa aaatcaaaag cagaatatgt
     3121 tcatacatta aatggttctg gattagcgat aggaagatgt ttggctgcta tattagaaaa
     3181 ttatcagcag gcagatggtt ctgtagtagt tccagaagaa ttaagacctt atatgggtgt
     3241 tgatgttata aaataaattt atattaattg tatatgtaga gtatgctttg atgaagagaa
     3301 tatattatga gtaaacaata aataaaaaaa ctatgaaatt attataaata ttaatttcat
     3361 agttttttta tgttaattta cacagcagaa ttttcatcat aaaacactaa atatttttat
     3421 aaaacactct tgtatgaata tctaaaaatt attttagaac taatttaaaa aaaatatagc
     3481 tatatatagt aataattaaa taaacaaaat aaaattaagt tgaattataa aatgaattat
     3541 gataagataa gtttgttata agttttggga ggaattatgg aaagttcatt ttatatgaaa
     3601 gaagcattaa aagaagcata taaagcatat aataaaaaag aaacaccaat aggtgctatt
     3661 atagttaaag ataatcaaat aatagctaga gctcataacc ttacagaaac gttaaaagac
     3721 tctacggcac atgcagaaat tttagcgata aaacaagcat cagaaaaatt aggtggttgg
     3781 aggttgactg attgtgattt atatgttaca atggaacctt gcataatgtg tagtggcgca
     3841 atagttaatt ctagaataaa aaagcttata atagggacaa gacatgtaaa aaattcatat
     3901 atagaaaaac agcatgagtt taaattagat tattttaata ataataatgt aaaagtagct
     3961 tttgatgttt tgcaagaaga atgctcgatc atattgcaag aattttttaa ggcattgaga
     4021 aaaagagatt aaggagagat ggtcgagtgg tcgaaggcgc tcgcctggaa agcgagtata
     4081 tgggtaaaac tgtatcgtgg gttcaaatcc cactctctcc gccagaaaca atgaaagtaa
     4141 ttaaatatgg ttgtaatttt gctgtactag atggggaggt agcggtgccc tgtaacctgc
     4201 aatccgctat agcaggattg aattccatct aaaggcttat tttctgtagg gccgccctaa
     4261 ataagtggtg ttgatgctta ggtcctgcgc aatgaaagct catgaacccc gtcagatccg
     4321 gaaggaagca gcggtaagtg atcacttttg tgtgccgtgg gggtgcctaa gttgagctaa
     4381 ctgttttggt aacgcctatg gaattaagtc gatagatggt gtacagcatt taatatataa
     4441 aagactaatc ccttagataa tgagggattt ttttattaat tttgctaaag agaggtaaaa
     4501 agatgcataa agcattatat agagcatata gaccacaaaa atttgaagat gttattggtc
     4561 aagatcatat tattaagact ttaaagaatc aaatatatag tgataatata ggacatgctt
     4621 atttgttttg tggaacaaga ggtactggta aaacatctac tgcaaagatt ttttcaagag
     4681 cagtaaactg tttgaataaa ataaatgaag aaccgtgtaa tgagtgtgag atatgcgaat
     4741 ctgtattaaa agataataca atggatgttg tagaaataga tgcagcttca aataatagtg
     4801 tagatgatat aagagagctt agagaaagtg tgaaatattc tccagccaat gctaaatata
     4861 aagtatatat aatagatgaa gttcatatgt tgtctcaagg agcttttaat gcattattaa
     4921 agaccttaga ggaacctcca tcatatgtaa tttttatatt agcaactaca gagcctcaca
     4981 aaatacctgc aaccatttta tcaagatgcc aaagatacga ttttaaaaga gttacagtta
     5041 aagatatgac ccttagaatg aaaaagattt gtgaagatga aggaattgat atagatgata
     5101 aagctcttaa tttaatagct agaaattctc aaggagctct tagagatgca cttagtatat
     5161 tagaccaatg tatgtctttt ggagaaagta agatagatta taaagatgtt gttgaattaa
     5221 tgggaagcgt aaatatagaa caattatttg aattgtctca atgtattgta gagcaggaca
     5281 ctaaaaagtc tcttgagata ttaaatgaat ttgtcttatg gggcaaagat ataagaaatc
     5341 ttataaatga cttaattgat cattttagaa atttaatggt atgtaaggtt tcatcagaat
     5401 tggatgaaat aatttcatta ccagaagaaa ctatagaaca acttaaaatt cagtctaaaa
     5461 atatagatat aaatgattta ataaggattt taaatatatt atcaataact caggatgata
     5521 taaagtcatc ttcaaatcca agggttttag tggaaataac cataatgaaa attgctcaac
     5581 caatgtttga tgaaagtaaa gaagctctta taaaaagagt tgagaactta gaaaaaatga
     5641 ttgaactggg gaactttaag tctgaaaaaa ttgggaataa taaagaaaaa gagtatgagg
     5701 tcgatagaga aatagatgta aaacaagaaa atgtagttta tgaagatgta aaaaatgagg
     5761 atgttatact tatagagtca tcttggaaga atatacttaa acaaattaaa aaagataaaa
     5821 aaatgccaat atacgcactc ttaagtgaag taaaaagttt taatgtatat tctaatatgt
     5881 tgtatgttat atttgatgat aaatttgatt ttgcaaaaac tagactgagc tctcaagata
     5941 caataaatta tttagaaaaa acaataagag atgttttaaa tagaagtttt aatgtaaaaa
     6001 tagtactgac atcagaagtt aaagatataa atcttgaagt taaagagaaa aaggacattg
     6061 gtgaggaaat attaaagaac atagtgagtg aagagatatt agaaataaaa gatagtattg
     6121 atgaaaatga gagtaaataa taaaatacta ttatgatata ataataaaat gaaaataagt
     6181 ttaggaggaa tagtgtttat ggctaaaaaa ggttttggag gcggaatgat gcctggtggt
     6241 ggaaatatga acaacttgtt aaagcaggct caaaaaatgc aagaaaatat gcaaaaagct
     6301 caacaagagt tagaaagtaa agaagtagaa gcttcagttg gtggaggagc agttactgta
     6361 aaagttaatg gtaaaaaaga agttatagat ataactatca agccagaagt agttgatcca
     6421 gatgatatag aaatgctaca agatttagtt ttaagtgcag taaatcaagc attgagaaat
     6481 attgatgata tacaagcaag tcaaatgagt aaggtaactg gaggtatgaa tatacctggg
     6541 ttattctagt aggtggtatt tatgcaagtt tatacaggtc ccataacgag acttatagag
     6601 gaattttcaa agcttcctgg agtaggaaga aagactgctc aaagattggc ttttcatatt
     6661 attaatatga atactaatga tgtggaagct ttatctaaag cgattataga tgcaaaaaga
     6721 gaaataagat attgctcaat ttgttgtaat ataacagata cagacccatg tagcatgtgt
     6781 tcaaataaaa gtagagattc aagtgttata tgtgttgtgg aagatccaag agatgtagca
     6841 gctatggaga gaacgagaga gtttaaagga cagtatcatg tattaaatgg agtaatttct
     6901 ccaatggatg gaattggacc agatatgtta aaaataaagg aacttatcca aagacttggc
     6961 aatcaggaag tcaaagaaat aataatggca acaaatccta ctattgaagg tgaagctaca
     7021 gccatgtata ttgctaggtt agtaaaacca atgggaatca aagtcactcg gatagctcat
     7081 ggtcttcctg ttggaggcga tttagagtat gcagatgagg tcacaatatc aaaagcacta
     7141 gaaggcagaa gagaaatata aaaatcgagg ttaatacctc gatttttgta gttttttgag
     7201 ttaaattcaa attaattttg atttagattt attgaataaa tttcttctgt attgtataat
     7261 ttgttgtata aatcatttaa aaattttgat aggggtgcaa catgaaacca attatgaact
     7321 tagaagaaca taatgcaatg aaagaaaaaa gagttaaaaa aatgaaatca aaagataaac
     7381 ctaaatatga tgattttgag gatataagtt tttctaataa gaaaaaaact aaaaaaaatg
     7441 tacgaaataa aaatgctgaa tttagaaat
//
LOCUS       DAEMGG010000156         7457 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEMGG010000156 DAEMGG010000000
VERSION     DAEMGG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.157"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            41..1018
                     /locus_tag="KPK24_003369"
     CDS             41..1018
                     /locus_tag="KPK24_003369"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG2301006.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1011..1979
                     /locus_tag="KPK24_003370"
     CDS             1011..1979
                     /locus_tag="KPK24_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG2301007.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2163..2753
                     /locus_tag="KPK24_003371"
     CDS             2163..2753
                     /locus_tag="KPK24_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2301008.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2772..3542
                     /locus_tag="KPK24_003372"
     CDS             2772..3542
                     /locus_tag="KPK24_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG2301009.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3679..4647
                     /gene="dusB"
                     /locus_tag="KPK24_003373"
     CDS             3679..4647
                     /gene="dusB"
                     /locus_tag="KPK24_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG2301010.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4756..5235
                     /gene="greA"
                     /locus_tag="KPK24_003374"
     CDS             4756..5235
                     /gene="greA"
                     /locus_tag="KPK24_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG2301011.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5250..6779
                     /gene="lysS"
                     /locus_tag="KPK24_003375"
     CDS             5250..6779
                     /gene="lysS"
                     /locus_tag="KPK24_003375"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG2301012.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7088..7282
                     /locus_tag="KPK24_003376"
     CDS             7088..7282
                     /locus_tag="KPK24_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301013.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2972 a          756 c         1511 g         2218 t
ORIGIN      
        1 gaaaataagt catgaaatta tgtcatatta gaggtgaaat atgagagata aaataataga
       61 gattatatta aataataaat ctgaatttat ttcaggagaa gaattatcaa aacaattagg
      121 ggtgtcacgt gcagctatat ggaaacatat gaaagcctta aaagaagagg gatataatat
      181 agaatctgta aataaaaaag ggtatcgatt agcggaaaat ccaactgatt tattaagccc
      241 acagaatatt tactataaat tagatacaga gtttattgga aaaaatattg aacatcttga
      301 cactatagat tcaacaaatg aacatgctaa aaggatagct ataaatagtg tagatgggac
      361 tgtgatttta agtgaacatc aagatatggg gaaaggacga ttgggaagaa gatgggattc
      421 aaaacctcat gaaggaatct ggatgagtat tatattaaaa ccagatatgg aaccattcaa
      481 agccccattt ataactttaa tagcaggagc aagtgtaacg aaagctttaa acaatctagg
      541 agcaaataca ttaataaaat ggccaaatga tgttatttta aatggaaaaa aaatatgtgg
      601 tatattaaca gagttatctg ctgaaattga aagaacaaat tatgttgttc ttggaattgg
      661 aataaatgtt aaaataatgg attttgaaca agaaatagct gacaaagcca catcattgta
      721 taaagaaggg tatatgttat ccagagtaga tattgttagg aatgtattga ctgaatttga
      781 aaaattatat ttagattata ctgtaaataa ttccaaagaa aaaaccttaa aaatatgtag
      841 agattattct gcaattatag ataaaaatat atatgtatta aaaggagata atagagaact
      901 agtgaaatgt ttagacataa atgaagaggg aaatttatta gttcaaagaa gtgatggaag
      961 tataaaagaa attatttcag gtgaagtttc tataagagga gaaaaaggat atgtataata
     1021 atattttgga tatagaaggc ttaaaggttg ggcaggttga ggatagaaat ggtcttactg
     1081 ggtgcacagt agtaatatgt gaagaaggag ctacttgcgg tgttgatgta aggggctctg
     1141 ctccaggtac tagagaaacc gatttattag accctgtaaa tatggttcaa aaagttcatg
     1201 cagtagtttt ggctggaggt tctgcatatg gtcttgaatc cacttgtggt gtaagtagat
     1261 acttagaaga aaaaggaatt ggatttgatg taggtgtagg taaagtgcct atagttgtag
     1321 gagcagtttt atttgattta ggagtaggga gctttaaatg tagaccagat ttaaagatgg
     1381 gttatagagc ttgtgaagtt gcaaatactg atattttaaa tcaaggaaat tatggagcag
     1441 gttgtggagc cactgttgga aagataagag gtcaggaata tgctatgaaa ggcggaatag
     1501 gtagctattc tataaaatta gataatggtc taattgtatc tgcattaatt gctgtaaatg
     1561 ctcttggaga tgtttatgaa aatggtaagg ttatagctgg tgctttaaat gataaaaaga
     1621 cagaaacaat tgatacatat aaattaatga aacaaggtga aagtaaaggt ggatttagtg
     1681 tagataatac tacaattggg ataattgcaa caaatgctaa gttaaccaaa gctggatgta
     1741 aaaagatatc tcaaatggga catgatggat atgctaagtc tatttttcct atccatacac
     1801 ctcatgatgg agatacaata ttcacaatat caacaggaga aatagaagca gatataacat
     1861 tagtaggttc attggctgta gaggttgtgg aaaaaagtat aataaatgca gtaaaaaaag
     1921 cggatagtgt agaaaatata atggcatata aagatatagt aaaaaaacga gaaatatgat
     1981 gaaattagca tatttttctt gtcaaattgt tgacaatggc aactcatctt gataaaatta
     2041 ttcttgttag agcgggcatc ctacaatggt agcttgactc atagttataa gaaaaaaacg
     2101 aaaaaaatta aaaaattaag tagtccggca tcttaatgag ctggaacgga ggtgtatttt
     2161 ttatgatgaa cacaagtgca tcagtaagaa aagtaaatgt aagaaagatg actataatag
     2221 gagtactatc agctatttct attatgctat ctatgactcc attgggattt attccagttg
     2281 gacctacaaa agcaactata atgcacatac cagttattat tggagctatt atggaaggac
     2341 caatagttgg ggcagcaata ggatttatat ttgggatatc aagtctctta aatgctataa
     2401 caaacccaac agtaacatct tttgttttta taaatccatt agtttcaata ttaccaagag
     2461 ttatgatagg tgtattagct tattatgtat atcagttaat aattaaggct acaaataagg
     2521 tatacatatc aggtttaatt acaggggcaa taggttctct gttaaataca gctggagttt
     2581 taggaatgat atatgtctta tatgctgaca agtatcttca tgcaatgggt aaaagtggtt
     2641 cagcaggaaa agtaataatg gctcttgccg cagcaaatgg ggtgccagaa gcaatagtag
     2701 gggctttagt agtagctgct gttgccactg tacttaagaa aagcaaaaaa tagaaagaag
     2761 gttaatgaca tatgcttcta gtatttgatg ttggaaatac taatatggtt ttaggtatat
     2821 ataaaggtga caaattagtt aattactgga gaattaaaac agatagagaa aaaacgtctg
     2881 atgaatatgg aatcctaata agtaacctat ttgattatga taatgtgaat ataagtgata
     2941 ttgatgatgt tataatatca tctgtagttc cgaatgttat gcattctctt gaaaactttt
     3001 gtataaagta ctgtaaaaaa cagccattaa tagtaggtcc aggcataaaa acaggtctaa
     3061 atataaaata tgataatcca aaacaagttg gggcagatag aatagttaat gctgtagcag
     3121 ggatagaaaa gtatggagca ccaagtatac ttgttgattt tggaacagca actacatttt
     3181 gtgctatctc tgaaaaaggt gaatatttgg gtggaacaat agcaccagga ataaaaatat
     3241 ctagtgaggc gttatttcaa agtgcgtcta aattacctag agtagaatta gctaagccag
     3301 gtatgactat ttgtaagagt actgtatcag ccatgcaatc tggaataatt tatggatatg
     3361 ttggtttagt tgacaaaata ataagtatga tgaagaaaga attgaattgt gatgatgtta
     3421 aggttatagc tacaggtggg ttagctaaac tgattgcttc agagacgaaa agtatagatt
     3481 atgtagatgg ttttttaaca ctagaaggat tgagaataat atatgaaaaa aaccaagaat
     3541 aattaatata cagggaattg ggttatatat ccaattccct ttttatgata gatttctagc
     3601 tatatgtggt atattataat gaggaaataa gaatttaaag ttaaatcaca aaataaaggc
     3661 aaaaatagga gaaaaacaat gaaaatagga aatttagaat tgaaaaataa agtttttcta
     3721 tcacctatgg caggtgtaac agaccttcca tttagattaa tttgtaaaga acaaggatgt
     3781 gggcttttat atacagaaat gataaatggt aaggcacttt gctatgatga tgaaaatact
     3841 aaaaaaatgc ttaagataga agaggaagaa catcctgtag cagttcagat attcggttca
     3901 gaaccagagt ttatgggaag agcagcagag ataatgaatg attattcaaa tgagattctc
     3961 gatataaata tggggtgtcc agctccaaaa gtagttaaaa atggagatgg ttcagcactt
     4021 atgaaaaatc ctaagcttgc agaagaggta ttgagggcag tagtgaagaa ctcaaaaaag
     4081 ccagtgactt taaagattcg aaaaggctgg gatgataata gtgtaaatgc tgttgaaata
     4141 gcaaagatag cagaggattg tggaataagt gctttagcaa tacatggaag aactagagaa
     4201 cagttttata caggcaaagc agactgggat ataattgcag agattaagaa aaatttgagt
     4261 atacctgtta taggaaatgg tgatgtattt acaatagaag attcaataaa tatgttagac
     4321 aaaactggtt gtgatgctat aatgataggt aggggtgctc aaggtaaccc ttggatattt
     4381 aaaagaataa accattatat gaatacaggc gagattttgc cagagccaac tcttaacgaa
     4441 aaaataagta ctgccattaa gcatttaaaa cttgcagtag aagaacatgg agagtatgta
     4501 gctgtaaggg aaatgagaaa acacatcgct tggtatttaa aaggacttag aaactctgca
     4561 aaattaaggg atgaaattaa caaaatagaa gactatcaag aagttgtgtc gaaattggag
     4621 tactatatgg aagactcctt gacataaaaa taatagtgac ttataatata gcgcataaat
     4681 taaaaaaaga ttataaaatt tttaaaatat aattaggaaa attcctaaga aatttgtgaa
     4741 ggagttgtat ttataatgga agaaaataaa gagtttttat tgacacaaga gggatatgat
     4801 aaattagaag aagaattaga aaacttaaag gttgtaaaga gaaaagaagt tgcagagaga
     4861 ataaaagttg ctatatcttt tggtgattta tctgaaaatg ctgagtatga tgaagctaaa
     4921 aaggaacaag cacaagttga agaaagaatt ttaaaattag aaaatatggt tagaaaagct
     4981 gttatcatag atgaaagcaa aatagacctt aatgttgtta caataggatc aatagttaaa
     5041 gttaaggatt tagaatttga tgaagaggtt gaatatacta tagttggttc gacagaagca
     5101 gacccatatg atggtaaaat atcaaacgaa tctcctgtag ggaaagcact tttaggtaga
     5161 gctgcgaaag aagttgtgga agtgcaagtt cctgatggag ttgctaagtt tgaaatatta
     5221 gaaataagaa gataatttgg aggaaaaaaa tgaaaaataa tcaacaaagc aatgaagaag
     5281 cgcaaattca agaagacctt agtgaagtac ttcaagtaag aagagataaa ctaaaaaaat
     5341 tacaagaatc aggtagagat ccttttaaag aaagtagata tgatagaact cattattcta
     5401 tggatataaa agataacttt gattctcttg agggtaaaac tacaaaaata gcaggacgta
     5461 taatgagcaa gagaatacaa gggaaagctg gctttataga tattcaagac caagagggaa
     5521 gaatccaatc ttatgtaaga ctagatgcta taggagagga agaatatagt gtattttcaa
     5581 catatgatat tggagatata gttggaattg aaggagaaat ctttaaaact aaaaaaggtg
     5641 aaatatctgt aaaggctaaa tctgtagtat tactatgtaa atctttacaa gttttaccag
     5701 aaaaatatca tggattaaaa gaccaagaac taagatatag acaaagatat gttgacttaa
     5761 tagtaaatcc agaagtaaaa aatgcttttt taattagaac aaaagcatta aaagcattaa
     5821 gagcatactt agatgataga ggatttttag aagttgagac tccaatatta aatactatag
     5881 caggtggggc aaatgctaga ccatttataa ctaatcataa tactttacat ataccaatgt
     5941 atttaagaat agcgaatgaa ttgtatttaa aaagacttat tgttggtgga tttgataaag
     6001 tttatgaaat gggaagaatg tttagaaatg aaggtatgga tttaaaacat aatcctgaat
     6061 atactgcaat agaattatat caagcatatg cagactatac tgatatgatg gaaataacag
     6121 aaaatgttat tgcacatatg gctgaagttg ctacaggaag tatgatagtt aactatcaag
     6181 gaacagaaat aaactttaca ccaccttgga aaagaatgag tatggaagat tgtgttaaag
     6241 aatattctgg tgtagatttt tctactataa atacagatga agaggcacta gaagttgcta
     6301 gagaaaaagg tatagagata aaacctggaa tgagaagagg agaagtaata aatgctttct
     6361 ttgaagaatt tggagaagat aaattaatcc aaccaacatt tataactcat catccagttg
     6421 aagtatctcc attatctaag agaaatgttg aagacccaag aagaactgat agatttgagg
     6481 catttgctaa taagtgggag ttagctaatg cattttctga acttaatgac cctattgacc
     6541 aaaaaggaag atttatagac caattaagaa aaagagaatt aggtgatgat gaagcttttg
     6601 aaatggatga agatttctta aaagcattag aggtaggtct acctcctaca ggaggactag
     6661 gaataggtat agatagagta attatgttat taactaactc tccatctata agagatgtat
     6721 tattattccc gactatgaaa cctattgata acaatccaaa taaagaagaa gaaaactaag
     6781 aaatattagc attctactaa gttttaaata aagtttagct attaaattat caataaatag
     6841 ctaaatttta tttaaaatgt cagtgtagaa tgctttttat atgcttcaaa gattctttta
     6901 gatatttaat ttataaatta ccaacaaacg ttagaaattg atattagttt ttgttgattg
     6961 agactttata tttagaaaat atgattgaaa gtaatttatt atgaaaatat ttagaataat
     7021 ttatgagaag gtaaaaagaa taatttatga gaatactata tataaaaaat tattggggga
     7081 atgtctgatg tttcaagtaa ttaatttttt atgtttattt tgtgttggat ttttggttct
     7141 tgatgttatg atatattttt gtaaggatgc aatggatatt tttgagggta caactaatgt
     7201 taataaagtt aagagtataa atgtttgggc agatagagtg agagctaatt catattctta
     7261 tgaaaataaa attgcaaaat aggtaaaatt atatatgcat gtattgtttg taatatcaag
     7321 ggttatatct attagtttta attatattta ttaaaaaaaa caaaagttat aacctttttt
     7381 aaaataaaaa acaaaaaaag tattgacgaa acatttttaa gatgatatag tattacttgt
     7441 ccttaagaaa agggcaa
//
LOCUS       DAEMGG010000157         7425 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEMGG010000157 DAEMGG010000000
VERSION     DAEMGG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.158"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(220..783)
                     /locus_tag="KPK24_003377"
     CDS             complement(220..783)
                     /locus_tag="KPK24_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301014.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(942..1655)
                     /locus_tag="KPK24_003378"
     CDS             complement(942..1655)
                     /locus_tag="KPK24_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG2301015.1"
                     /translation="MGKVAKILLSLIVIVLVLVGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1947..3611
                     /locus_tag="KPK24_003379"
     CDS             1947..3611
                     /locus_tag="KPK24_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG2301016.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(3718..4617)
                     /locus_tag="KPK24_003380"
     CDS             complement(3718..4617)
                     /locus_tag="KPK24_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG2301017.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(4644..5282)
                     /locus_tag="KPK24_003381"
     CDS             complement(4644..5282)
                     /locus_tag="KPK24_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG2301018.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(5623..6621)
                     /locus_tag="KPK24_003382"
     CDS             complement(5623..6621)
                     /locus_tag="KPK24_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG2301019.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(6719..7294)
                     /locus_tag="KPK24_003383"
     CDS             complement(6719..7294)
                     /locus_tag="KPK24_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301020.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         2538 a         1168 c          810 g         2909 t
ORIGIN      
        1 ctatccatcc cgcacctaaa ggagtgggat ttttgcacta ttctgtatta tatatatcca
       61 aaaatattat aaaaatacaa atattagcag aaatttaaaa actgaagaaa gtattctaac
      121 taagatttac aaattcttaa tcctatcgaa gttaaactac tatcttcagc ctaattatct
      181 agcaatattt ttttatatga aaaactctaa atcaagtttt taaatgtttg cgatttttga
      241 tacataagaa tgttgtctag aattatattc tgataataaa ccttcatact ctaaggttaa
      301 gccagataaa ttataaaaat catttggcgt tacttcaaat gcttgtttat ttaaaatgtc
      361 tatctcactt ttttctaaca tgtcagcaac aaaatttgaa catacatatt tatattcaga
      421 ttgtctctga atattcatta gtaaatatat taatgccatt gtatcgtaat agtaatcttc
      481 tctataatca ctaattaatt ttatattttt atatagattt tcatattgta aattatctac
      541 ttctaacgaa tataccctac acacagtgtt ttttcttata gcatataatc cttgatttat
      601 attttcttca acaaatcctc caataaatgg attccttgga tttagccttc caaaagaata
      661 cattggtttt agattttcat ttaatgatat tgacacatgt gtatacagct ttttacttat
      721 attccttatt aagtaagata atatagttcc tgtatatgtt gttacaatat agactttatt
      781 catcatttcc ccccaatcta gtaactaaat aatattatac acttacaatt taatatcata
      841 agatattcta acataataaa tgtggtttta caaattttga ttttaaggta ctttaatatt
      901 atataatata ataaaaatat aaatataagt tttctatatt tttaaccaac agatgttttc
      961 ttcaaattaa tcagatgatt tagtatatct tgaacctttc cagagtcctt taaatatcct
     1021 aatgccccat ttatttgtga ttttaaatct atttctactt ccatatccaa atcaacattt
     1081 ccttttttta tagatatatt tttaagtgtt attggataaa gataactttt ttccaaagta
     1141 atcttatttc cattaacctc aaatggaact tttttattgt agttacttaa tattttttct
     1201 agcattttat cacttagttt aaattttcca attttcacat tctcaagact tacaactaag
     1261 tttccatcct ttaaagtagg tctcaattca agttcatatt gactatttac caaaccaaat
     1321 accttatatg gtcctgctac ctttatttta ccatttttca tttcaacaaa attattttca
     1381 agttcattaa ttccatgctt ctgcatcaat gtatatatta aatttttaaa ctcttcctct
     1441 gaaacagata tttttccaac cattctaagt ggatttttta ctacctccat agaatttata
     1501 tatgagcctt ttgataaata ttcttcatct atgattttat tattttgtga actaacatca
     1561 tatctttctt ttggtgtcaa gatatataca acaattccta caagtactaa aacaataaca
     1621 attaaactta acaaaatctt tgcaaccttt cccataatcg tctcctttac aaatctgtca
     1681 tcagatttaa aataaattta gataactaac ttcacataat attcttaaca atatacatac
     1741 tataatacag tcttttaaaa attaattcaa ccaatctaat gttttgtaaa taaactgtaa
     1801 aaacatgttt ttagtataaa agttaatatt ttgcataata ctcttaatta cacctttatt
     1861 tttttctaat cttggattaa aattaatatt atgcaataca gtgtagtgtt ctgtattata
     1921 atcaaattaa ataggaggta tagaatatgt caaatgaaac gaactcatct aactttataa
     1981 agaatattat tataaacgac ttagaaacag gaaaacatga cagtatcata actcgttttc
     2041 cacctgagcc aaacggatat ttacatattg gtcatgcaaa aagtatatgt cttaattttg
     2101 gattagctaa ggagtttaat ggaaaagcta atttaagatt tgatgataca aacccattaa
     2161 aagaggatgt tgaatatgta gagtccataa aagaagatgt aaagtggtta ggatttgatt
     2221 ggaacgagtt aaattttgca tctaactact ttgatgaaat gtataagaga gctttaatac
     2281 taataaaaaa gggtaaagct tatgtatgtg atttgacaca agaggaaatg agagaatatc
     2341 gtggtactct aactgaacca ggaaaagaaa gtcctcatag aaacagaact attgaagaaa
     2401 accttgattt atttgaaaga atgaaaaatg gtgaatttaa agatggtgaa aaaacattaa
     2461 gagccaaaat agatatgtct tctccaaata taaaccttag agacccaatt atatatagaa
     2521 tatctcattc aacacaccat aatacaggag ataaatggtg catatatcct atgtatgctt
     2581 ttgctcatcc aattgaagat gcaattgaag gaataactca ctctatatgt actttagagt
     2641 ttgaagacca aagacctctt tatgattggt ttgttaaaga atgtgagatg gaaaatatac
     2701 ctagacaaat agaatttgct agacttaata taaataatac tgtcatgagt aaaaggaaat
     2761 taaaacaact tgtagatgag ggtattgttg atggttggga tgaccctcgt gttcctacaa
     2821 tatccggaat cagacgtaag ggatatactg cagaagcatt gcgcaatttc tgtagtgaaa
     2881 taggggtatc aaaagttaac tctacggtag atagtcaaat gcttgattat ttcctaagag
     2941 aaaacttaca accaaaggct cctcttacta tgggagtttt aagaccttta aaacttatta
     3001 taactaatta cccagaagat aagattgaga tgcttgaaat agagaataat gctaaagatg
     3061 aatctcaagg aaagagatta gttcctttct ctagagaatt gtatatagaa caagatgatt
     3121 ttatggaaga acctgttaag aaatatttta gatttttccc aggaaatgaa gtccgtttaa
     3181 aaggtgctta ttttgtaaaa tgtactgatg taattaagga tgaaaatgga aatgtagttg
     3241 aaatacatgg tacttatgac cctgaaacta agagtggctc tggctttaca ggacgtaagg
     3301 taaaatctac aatacattgg gttgatgcta aatcagctat cccatgtgaa tttagattat
     3361 ttgaaccatt aattcttgat gatatacctg aaaatgaagg taaacatttc ttagaacaaa
     3421 taaatcctaa ttcattggaa atattacaag gttttgttga acctacacag ataaaagatg
     3481 ctaaaccttt tgataaattc caatttgtta gaaatggatt ctttagtata gataataaat
     3541 atacaactga tgaaaaattt gtatttaata gaatagttcc tctaaaaagt tcttttaaac
     3601 caggtaaata atcttacaat caaaaaatga agacttaaaa tataaattac ttctactata
     3661 aataaactta gttagaagta attttttatt tgagtcttca tcatttactt taaatgatta
     3721 acttttttca ctacagattc ccatgacttc tattccatct tttatgcatt ctatgttaag
     3781 aggaaaatct ggtccttttt ctccgtcaat atctgttata agtgaatctt cacattctat
     3841 atgcaccttt ttagttttaa agtatagtat ttcatttcca ttatattgat ctaaataatc
     3901 acctctaagc acacttatca aaactggtat tgattttggt attggcattc ctttaaaaat
     3961 aattacatcc aaatatccat catctatttc agccttatat gctagattta tatttcctgc
     4021 tgttttgcca ttaaaaatca acataagata catatctcca tcataataca tttcctcaga
     4081 agatactttt atcttaaatt tcctcatata caatgcttct tctattccct ttatataata
     4141 agaaattctt ccaaatgaat tttttaagtc tgtgtttatc ctttgtgata catccgtaaa
     4201 cataccagca cttgcaacat ttataaagta cttattattg actttcccta tgtctatttt
     4261 ctttggtttt gaatttatga tttcatttat agcttctttt atatcaaatg aaatttgaag
     4321 agcatttgaa aaatcatttg cagttcctgt aggcaaaatt ccaattggaa tttttatgtc
     4381 taattctttc attgcattta ctactaaatc tacagtacca tcaccaccag ctatcaatat
     4441 gtgttcataa gaactatcaa cttccttaaa tgcattcttt acaggttctt taatagttaa
     4501 tctatagggt atcaataaat aatttttttc ttgatataat tctatgatag tgtctaaatt
     4561 tgataatatc tttttttcac ctgaattagg attgtatata agttttactt ttttcattat
     4621 ttctccttac tcattttatt cttttagtat ccaaaatctt catttaaaaa aatcacatgt
     4681 attctttctg gtgttaattg tctttttata agtgtataac tacagcatat tcttcctgga
     4741 cttgaacagt ctatacatat accagtagtc ttacatccag tatttgtact taatctttta
     4801 caattggctg gagcagctat tgtttttgtt ctttttatag cttcatctac atctttaact
     4861 attttattta ctcctgctat aactattact ttatcaggtc catatatcat agcagcaacc
     4921 ctattcccat tcccatctaa attgtaaagt tctccatcct ctgttatcgc attagtactt
     4981 gtaaaataag catctgcaga aaatgacttt ctatatattt ctttcatttc ttctggtttg
     5041 atatcttttg catatctatc aagaaaatta tatttttctc ttataaaatc aatcactcct
     5101 gtttcaaata aggtcataga acctccacat cctactagag aacctgcttt aactatttct
     5161 tcaatttttt taagaagctc ttctttgtta cttactaaat accctttcat attattgttt
     5221 tccaaagatt ttattgtttt ttctattctt ttttcattaa gccaatttaa attttgatcc
     5281 attttaactt cccccttatt tttaatatgt acttaattat ttataaactt tattttcttt
     5341 ataagtttat tttagtacat ctaaaaaaaa atacaatata aaacttaaat agttttacat
     5401 aattttttcc aaaataactt actcaatcaa aaaaggaaag tagatgtact attctacttt
     5461 ccctatattg tatgtagtat tatatattgc ctaaaatcta tatattaatt atatgtttca
     5521 tattatatga atatcacagt taattaaaat gtaatactat cttcttttta aacatataaa
     5581 ttgtattatt aaatttaata tatccaactt caataaatct tattaatttg atgaagaaga
     5641 tgctatagct gcacttgttg ctgccattac acatgcaatt tgaatagcaa caacaatatt
     5701 taaaaatata ttattagtta tagagcctat attattctca tttcgtatca aatctgcata
     5761 aattgaagct acaatagcag aactaatcat tactctatta ctttttccta aagaccaatt
     5821 tccaaagcct ttttcttctt taagtttatt tgaaacattt tttatctctt ctattatttc
     5881 atcttctcta taatctaata atgaaattgc tcctattatt ggatatccat aataattcat
     5941 tttacaatca ttttctttaa atgctttttt aagttcaata gtctttttac atttgagcat
     6001 agttctatca tcactaaata aaagaatttg ggataatgct tgtaaatcat ttcctttata
     6061 aaaaccattt tcatttagaa attcataaca tttttctatg tattctaaat cttcatctaa
     6121 attttttgaa tgaattgcaa taagtgctat attacaatag tcatcactag aagttagaaa
     6181 tggatgtttt ttcctcatgt aatcatatat tattttcatt tttgatatac atgtaagata
     6241 atccattttc tctctatttt cataaactat tgatgctgta agtggtaaat atgtatcgcc
     6301 ccaaaacttt tcacttttaa gttctttgta tatatctaaa atcatcttaa aactactttc
     6361 tggatttgat tcaaacgaaa gtagtgttgc catataaaac atattatatc ctctaaaatt
     6421 agagaataat ccagtatttt ttttaataag ctctattcgt tcttccacta atctaggact
     6481 tattctcttg ttcttaaggg taaatgcaag tgcacaacta tgttgtatca atcccatttg
     6541 ccaagaacct ttaacttcat ttaatgcttt gtaattttct attaataagt ttacttttcc
     6601 atttataaac gattgttcca tattgccctc ctagtaatat atctcattgt actaatagtt
     6661 ttatatttat tatatatata ttaaatacta caatcaatac atattacttc aaagaacatc
     6721 attcttcaac aataatgtag ccttttttgt aatgtcttcc catattattg actctatcat
     6781 agtactctga ttcactttca ttactccatt tgggcacaaa tacatctatt gcattagaat
     6841 tactaaaata tttactagac cccctatcca taacagtttt taattttcca tctacaataa
     6901 tcttagttcc aaaacttaag tgattgtttg ctacaaaacc atcttctaaa tcatctcctg
     6961 tagcagttat cgctccatat ccattttctt gattaagact tgtgtaataa ctaacttcaa
     7021 aagttatagg tactaacttc tttattttaa tacctaactg attttgtagt ttctcctttt
     7081 gtactttcaa cttatactca tcactatttt tataatcttc cataccttga ttatacttgg
     7141 aattaaagct tttacctgac tttactatag tatctatatt tactatagat tctttacaat
     7201 gatctgcatc tatttttgtt tctccattta ccaataatgg agttaaacat acactacata
     7261 caacacatgt aatcaatgct tctttaagct tcataaattc cccccttatt attttgatat
     7321 attagcatat ttaatattat atgtctacat ttagatttaa ataccacacc catttgcttt
     7381 aagaagttgt cttatattta attcacctta aacacttata aatcc
//
LOCUS       DAEMGG010000158         7274 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEMGG010000158 DAEMGG010000000
VERSION     DAEMGG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.159"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..358
                     /locus_tag="KPK24_003384"
     CDS             <1..358
                     /locus_tag="KPK24_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2301021.1"
                     /translation="LSTKLIKENDIICIEDLQVKNMIKNHKLSRLISDVSWSEFIRQL
                     EYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINAS
                     INILKEGLRLITIQNK"
     gene            485..1654
                     /locus_tag="KPK24_003385"
     CDS             485..1654
                     /locus_tag="KPK24_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG2301022.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(1817..2359)
                     /locus_tag="KPK24_003386"
     CDS             complement(1817..2359)
                     /locus_tag="KPK24_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG2301023.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2507..2791)
                     /locus_tag="KPK24_003387"
     CDS             complement(2507..2791)
                     /locus_tag="KPK24_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2301024.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(2854..3618)
                     /locus_tag="KPK24_003388"
     CDS             complement(2854..3618)
                     /locus_tag="KPK24_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG2301025.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(3900..4166)
                     /locus_tag="KPK24_003389"
     CDS             complement(3900..4166)
                     /locus_tag="KPK24_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2301026.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(4208..5017)
                     /locus_tag="KPK24_003390"
     CDS             complement(4208..5017)
                     /locus_tag="KPK24_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301027.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIEELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            5330..5611
                     /locus_tag="KPK24_003391"
     CDS             5330..5611
                     /locus_tag="KPK24_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301028.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            5875..6129
                     /locus_tag="KPK24_003392"
     CDS             5875..6129
                     /locus_tag="KPK24_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301029.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            6174..6497
                     /locus_tag="KPK24_003393"
     CDS             6174..6497
                     /locus_tag="KPK24_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301030.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(7056..7250)
                     /locus_tag="KPK24_003394"
     CDS             complement(7056..7250)
                     /locus_tag="KPK24_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2301031.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT         2689 a          869 c         1022 g         2694 t
ORIGIN      
        1 actatctact aaattaataa aagagaatga tatcatttgc atagaagatt tacaagtaaa
       61 gaatatgatt aaaaatcata aactatctcg cttaatttca gatgtatcat ggtcagaatt
      121 tattcgtcaa ttagaataca aggctaattg gtatggtaga caaattgtaa aggtaggtaa
      181 attctttgca agttcacaaa tatgcaataa atgtgggtat aaaaacgagg aagttaagaa
      241 cttaaatata agagaatgga tttgtcctag ttgtaatgaa actcacgata gagatataaa
      301 tgcaagtata aatatattaa aagaaggatt aagactaata acaattcaaa ataaataacg
      361 tatgtaagaa ccggaggaac tacggggata gcctgtggag aattagtaag acatatttta
      421 gtatgcaaaa ttctgtgaag caggaaccct gtgactttag tcatgggagg ttcagggtag
      481 aaagataaat gaactttgta aagatgaagt tgtagagttt tgcaatagaa taaaaggtaa
      541 agtggaaagt gttgcaataa gtggagtatt tgctccagta aataaacaac aagaagaaga
      601 agttgcaaaa tgggttagag aaattttagg ggatattcct atatctttat caaatgaaat
      661 aggtaatata ggtatattag aaagagaaaa tggagctatt ttaaattctg cattgtctca
      721 agtaggtaaa gctgtatctt taggatttga aaaagcactt aaagacttag gtatagatgc
      781 tcaaatatat ttcagtcaaa atgatggaac attaatgaat ttagagtata caatgaaata
      841 cccaattttt acaattggat gtggaataac aaattctata agaggagctt ccttcttatc
      901 aaaagtaaaa aatgcaatag tattagatgt tggtggaact acaagcgact tgggtgtact
      961 atataatgga ttccctagag aatcatctat tccagttaca attggaggag tacatacaaa
     1021 ctttagaatg ccagatgttt taactattgg tcttgctggt ggtacatgtg taaaaggcga
     1081 aaaagataat ataaagatag gtcctgaaag tgtgggatat agaataactg aagatgcaat
     1141 aatatttggt ggagacactc taactttatc agatgttgtt acaaaattag atatggcgtt
     1201 tgaagataga aaaagcaatg ttgaaaactt agataaagag tattgtaatg aggtatataa
     1261 gaaggttatt ttaaaattag aagatgctgt agaccaaatg aagactagtc aagatgatgt
     1321 tgaattggtt ttaactggtg gaggaagtat aattgttcct gaaaaactaa aaggcataag
     1381 caatgttata agacctcctc attatactgc tgcaaatgct attggtgcag ctttaggaca
     1441 aataagtgga gatgtagaaa aagtatacag tttagataaa atgtcgagag aagatgccat
     1501 agaagatgca aaaaaagagg ctgtaaataa agctctaaaa gctggtgcta aagaagatac
     1561 tatagaagta ttaaatatag aagatgttcc acttgcatac cttcctggta atgcacttct
     1621 tataaaagta aaagttgttg gtgatttaat ttaaagaata aatataagta tttccagaat
     1681 tatataaata tttctggact catttttaaa cttattttat caataaaaac agcataactt
     1741 ttgactatat tcttataagt ttccgaacag ccgatatata cctatttttt gaggtttatc
     1801 ggctgtttgt tatcttttat cgcattccaa ctatttttct taccgtattt gctgtacgaa
     1861 ttgtaatctt atcacttaca tttgaactgg cagtctttga ccaccaattt gtctttttat
     1921 aatctttacg actaaaagac caaaagatag catttttata atgatatact ttttcatact
     1981 cttctttagg atttccaacc tcatcataaa actcgtcata agataagtca ggaaacataa
     2041 aaataagatt atcatatatt tctttgttgt tatctcccca ccaattagga gcattattta
     2101 gtatatcttt taactcttct tgtgaaataa taaaaactgg tatttccaat tcaaactgtt
     2161 tttttatcat taatcttatt gtatttgaga taaccttgct gtcatctaca tcacttaaaa
     2221 agacaacatt accactatta aggtatgtag aaacttctga ataaccaagt tctacaaagc
     2281 ttgcttttaa ttcagacata attatcttgt tcttaccact tatattgata cctcttaata
     2341 atgcgatata acttttcata taataatttc acctctaaac attcacattt ttctatgtag
     2401 tacaaactgg aatttgcatc ttttataatt ctttcaaaac ttcttcatat gtaatttttt
     2461 taatataaaa aaatcggtgc ttaatttgta ccgatttttg tttttcttag taaagaactc
     2521 gtttttttgc ttctgctata gctctagcag cactttttgc ttgcattctt ttttcttctt
     2581 cttttttaaa ttcatttatt gtttcattta gattcttatc atgttcattt ttttctttat
     2641 cttcgttttc tctgtatact aatatataat ctattattga ttcgtttatt atttgagata
     2701 tacttttatt ttcattaatt cctattctct taacttcctt aagtagagtc tcatcaatat
     2761 gaaccgttgt ttgctttgta ttttttgcca ccttaatcac ctcttaaaaa aaattctaac
     2821 attaaaattt tttttagtca ataaatttta atattaaaat tctttaaatg ttttatttga
     2881 ttttctaaaa ttttctattg tatttatcat cttatttttt atattttcac ctagtacaga
     2941 agactttagt ctaggatttt cgatatggtc ataaattggt tctaaaaatt cgacttcgac
     3001 aggaacagag ttaattttac caataggttg gtatccttca taagtatctt ttgaattttt
     3061 tattactagt ggaacaatag ggcattttgc tttatatgct atctttaaag ctccacttct
     3121 aaactccgaa accaatgaat ttggttcttt aatccatgtt aaatctcctt ctggaaacac
     3181 tgccatactt tgacctgtaa gtatattttg agaggcttgg gctatactct ttataccctc
     3241 acgattgttt tttctgttta catatacaca tcttagtaat tttgcccatt ctttaaatat
     3301 aggtatattt ctccaaactg gttcatctgc aattacacat ccaattggtc tttctacact
     3361 tgctgttagt atgaagctgt caagcatact agaatgattt acaacaaata gaagtggttc
     3421 ttttggcagt gtttccttac caattatatt aagctctatg ttcactatat ctaaagattt
     3481 tttagcttgt ttttgcataa attcaaattt ttcctttaag ctaaatttat caggattctt
     3541 tttaatttta ataagcctag gtatagaaga tgtaaaacct ataagttgat atgtagcaaa
     3601 ttttaagtaa ttattcataa aattatctcc ttaaaataaa attatttatt actgtaaaga
     3661 ttttaacata aaataaaatt taagtctatt ttaattcaat tttgtaacat atattttaaa
     3721 tagacagaga ttgagatttt atatttccaa ttttagtaat cagattttat caataaatta
     3781 ctattctatt aatttaatta aataaccttt aaatttaatt ataaaaactg ttgcagaaat
     3841 ttctacaaca gtttaaaaaa tttaccttac aaaaacttat tttataacaa gagagtatat
     3901 tataaggcta gtacagccca ttttttgcac tgagaaattc ttttgccatc atcaccttgt
     3961 tctttgtcat atgcaaattg attaagcatt tcacatggaa atgtatcaca tagaccacaa
     4021 tgtgtaaggt ttttatcttc acaacaagat tttacaggac attgttcacc ccaaaatggt
     4081 ttctcaatct ctgtacaacc tttacaattg actttttcct tgtatccaca ttcataacat
     4141 aaaattccac atcttgattc tatcatttta agtcactcct taaatataaa ttttatatga
     4201 ttgtatatca aaatttatac ttttcattgt aaaaatcgga caaatttttt atataggttg
     4261 ttggattaac accacaaata gatttaaaat catttataaa atgagattgg tcgtaataac
     4321 caatatcttg tgctaaagtt gcaaatggta ttttattatt ttgtatttct tgtaatacaa
     4381 ggtttatacg aagtaatcta aggtatgatt taacactcat accaagagaa ttattaaaaa
     4441 ttctatttaa atgtcgttca ctataacaac taatttgtga aatgttttta acagacataa
     4501 tagagttttg ttttctagca ttttcaagaa ttggtatagt catatctact atatttgatt
     4561 tcaataagta tgataagaat aacatatcta attgttctac taattcttct atagtacttg
     4621 ttctttcaaa aatggaatta acctctaagc taaataatgt gttaaaatct tcaagtgaaa
     4681 tttttaaatc tgtagattct ctttgagaaa gacctgtcaa ataatatact ccacctgggc
     4741 gaaactcaac gaatactcta aaaagtacat tttctatatc attttttaca atagtagttt
     4801 ttgttgtggc tccccagaat gcgctttcta tacctttttt gtcaaattta aatatcatac
     4861 atccactagc atcaggtatt aatgtaagat tttctttaat acttgtatct ggaatagata
     4921 ttgtatagtg tgctatatac tgtcttaaca atttatgtgg caaaatatat gtatactgtt
     4981 tgcaggtatt taaaatgtta ttgattattt tattcataag tatatcctcc tgatatataa
     5041 aaaataagta tactaattta ttttttatat aataaaccag ttttgtttta attgaaatat
     5101 tctaataaga tattttgtta aatttagata caataattgt aaagtaaaat ctcaaaatat
     5161 acaattatta taaatcatag atatttatta aataaatata ttaaagaaga aggaatataa
     5221 aacaagatta gaaaataata gtgttacttt cataataaat acatataaag atatttttaa
     5281 gaataaaatt tattattaaa taaaaaataa aggagagtga tttaatagta tggcatcaaa
     5341 acaaacacca agaaacaatt tagaaaagtt tttaagaata agagtattat cacatacagg
     5401 cgataaaata catataaagt tacctgtaaa ttttgcaaag agaatgattg agaataatgc
     5461 cttggattta tttaatggaa aagatgatgt agtagatggt aaaaaagtta ctgaaatatt
     5521 agttaaggca tttgactatg atttaactgg agaagtagtt cacttggaga gaaaaaatgg
     5581 agacattatt aaagtttcaa ttgtacaata gaattatttt atatgaattt tatttttgaa
     5641 tagttataaa taattagaaa tttgtaaaaa agtataatat attattagta atggtacaga
     5701 atacacattt ataatttata gatgtgtatt ttttgtttaa aaaatttaaa tatataaaac
     5761 tataaaggtt acaattttgg aaatagatgt aaatatatgt tgtattgtat aataagagta
     5821 atataaaatc tagtaaaaat aatcaaataa atttagctta tgagagggat aactatggaa
     5881 aataaggatt ttaaattaac aatatgtata tttgcttttg taattggagt agtagtatta
     5941 ttctgttgta taggtttagg tgaatttagt gcagagcata ttattcaaca aaatggtgga
     6001 atcatggata ctgaccaata taatatttat ctagaccaat ctatttctca atatagaaat
     6061 tttggttcta tattagctct gttaggagga atagggatat tgtttgataa atatatcaga
     6121 aaatactaga gattatgttt tgtctaaaat aaattcttat gaggagataa aaaatggcaa
     6181 cagaaatatt tactgtaaaa attacaccct tagaggcaca aaaaatgata aaagaaaatc
     6241 ttgatgcaga tttagttttt agtgattctt ataatttagg agatgataaa tttatactaa
     6301 ttacaacttt tgagaagtat tatgcaagaa ataatagtga tgcaggatta atagtagttt
     6361 atgaaaatac cacaggaaaa acagttatta aagctacttc aacaggcagt gcaattggaa
     6421 ttttaaaaat tgattggggt gcaggtggaa atttacttaa aagagtaaaa aacatacttg
     6481 ctgattatat tatttaatta ttttatctaa ctgataatat gtcaagaaaa tctcgtaaaa
     6541 gaggttaaga acatttttat aatatataga taaataagaa tttgaaggta aagtttttaa
     6601 ggtataaaga aaaataatat aataaaacga attggcttta taattcttta taaggcaaag
     6661 agcacatttc tttatataat tgttggatat taaataatat gtaaatacac catttacata
     6721 taaaaattta attaatatgt taagagaaaa tgaaatggaa attactaaaa aatattttta
     6781 aattggataa tggttgatat tatgtttata caaaaatcat gtggaatttt atatatttta
     6841 aatatgatag tttaaaatta tgaaagaatg ataaattcca gtttgtaggg taattttgat
     6901 aaataatatt aataagtatg agtaaaaagt aggagcatat taatataatg tccctacttt
     6961 tttattatta aaattgttta taatatcaac actacattaa aacacaacct aaatttgaaa
     7021 aacaaatagt ttagaaatat gtactttttt atctattata ttcttttaaa tcgaattttt
     7081 tcatctgaac ataaagtctc aaatctatat gcgtctgtca tatcataaaa accaaaaaat
     7141 ataaagccat cacgagaagt aaattcatat tgtatatttt caattttcaa catgttttct
     7201 acttcgctta ttttttcgct atatataatt tctaccatat aatttatcat tgtagaatcc
     7261 ctcctattaa tatg
//
LOCUS       DAEMGG010000159         7223 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEMGG010000159 DAEMGG010000000
VERSION     DAEMGG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.160"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            477..3119
                     /gene="adhE"
                     /locus_tag="KPK24_003395"
                     /gene_synonym="adhC"
     CDS             477..3119
                     /gene="adhE"
                     /locus_tag="KPK24_003395"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG2301032.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3479..3820)
                     /locus_tag="KPK24_003396"
     CDS             complement(3479..3820)
                     /locus_tag="KPK24_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301033.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3847..4518)
                     /locus_tag="KPK24_003397"
     CDS             complement(3847..4518)
                     /locus_tag="KPK24_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2301034.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4530..7106)
                     /locus_tag="KPK24_003398"
     CDS             complement(4530..7106)
                     /locus_tag="KPK24_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2301035.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVILANIIVGILATYIPSRKINKISIVEAINI
                     TE"
BASE COUNT         2542 a         1024 c         1061 g         2596 t
ORIGIN      
        1 agagattaga ataattttag tttatatatt ttttaaaact atacagttgt tataaaaaga
       61 aaaattgcat acaagtcaaa aataagtgtt attttataaa aatacgacaa aaattttatt
      121 tactacttat acaaataaca tgcaaataac atataaaatt taataagtaa aaaaaatgaa
      181 agatataaat tatcatttta gtattttatt tagtcgaagt aaattattca aatgaaatat
      241 atctcattta gataaaaaat tttagtaaaa gtaaaaaaaa taatatattt gttaaaaaat
      301 aaattgacaa aatattcaga aaaaaacttc ttgacctaag agagtaaaat aaataaaatc
      361 taaggtatag agatgaaatc tataaaatta ttttaagtat aagttaaaaa tatagatgat
      421 gcatttaaga ttaataaaaa ttagagatgc ataattattt tagggaggtt tttagtatgg
      481 agaaaaaaga aaaggtagta gaaaaatcaa atgttgaagt atgtagtcct gaaattgtca
      541 acagtgttga aacattaaga atgagattag aagaaattag attggcacaa aaagaatttg
      601 caacatttac gcaagaacag gtagataaga ttttcttagc tgcttcaact gcagctaatc
      661 aacaaagaat acctctagca aaaatggcag tggaagagac tggtatgggt attgttgaag
      721 ataaggttat aaaaaaccat tttgcctcag aatatatcta taatgcttat aaagatacta
      781 aaacttgtgg tgtcatagaa aaagatgaag catttggttt tacaagaatt gctgagccag
      841 taggtgtttt atcagcagtt ataccaacaa ctaatcctac atcaactgca atattcaaat
      901 ccctaatagc actaaaaact agaaatggta taattttctc tcctcatcca agagctaaaa
      961 actgtactat agaagcagct agagttgtac atgatgcagc agttaaggca ggagcaccaa
     1021 aaggacttat aggttgggta gatgttcctt cgattgaatt aactaatgtt gtcatggcag
     1081 aggcagacct tatattagct actggtggac ctggaatggt taaatctgca tactcttcag
     1141 gtaaaccagc tgtcggagtt gggcctggaa atgtacctgc aataatagat gagagtgcag
     1201 atataaaaat ggctgtaagt tcaatcttag tatcaaaatc attcgataat ggtatgatat
     1261 gtgcttccga gcaagctgta atagtaccag aaaaaatata tgaagaagtt aaaaaagaat
     1321 ttaaatatcg tggagcacat ttcctaaata aagaagaaac tgaaaaagta ggaaaagtag
     1381 taataattga tggttcatta aatgccagaa tagttggaca accagcacat gtaatagcca
     1441 aaatggcgga tgtagaagtt ccaaaaacag ctagaataat aattggtgaa gtagaatctg
     1501 ttgaattaaa tgaacctttt gcacatgaaa aactttctcc tgtacttgca atgtacaagt
     1561 ctaaaagttt tgaagatgca gttgctaaag cagaaaaatt agttgcagat ggaggctatg
     1621 gtcatacatc ttcattatat gcagactcta taaatcatcc agagagagta gaaaagtttg
     1681 taaatgctat gaagacttgt agagtattag ttaatactcc atcatcacaa ggtggtatag
     1741 gtgatttata taactttaaa ttggcaccgt ctcttactct tggttgtggt tcttggggtg
     1801 gaaactcagt atctgaaaac gttggagtaa aacatttgct taatatcaag acagtagctg
     1861 aaaggagaga gaatatgctt tggttcagag caccagaaaa ggtttatttc aaaaaaggat
     1921 gtttaggtgt agcagcaaga gaatttaagg atgttatgga taaaaagaaa gcatttatag
     1981 taacagattc tttcctatat aataatggat atactaagaa attaactgat ttattagatg
     2041 agatgggaat taagcatact acattctttg atgtagctcc agacccaaca ttagcttgtg
     2101 caagagaagg agctaaagct atggctgatt tccaaccaga tttaataata gctgtaggtg
     2161 gaggaagtgc aatggatgct ggtaagataa tgtgggtaat gtatgagcat ccagaagtag
     2221 atttccaaga cttagctatg agatttatgg atataagaaa aagagtatat gtatttccta
     2281 agatgggtga aaaagcgtat tttgctgcta tcccaacttc agcaggaact ggttcagaag
     2341 taactccatt tgcagttata actgaccaag attcaggtgt aaagtaccca ttagcagatt
     2401 atgaattaat gccaaacatg gctataattg atgctgatat gatgatggag atgccaccaa
     2461 gattaacagc agcatcaggt gttgatgctt taacacatgc attagaagca tatgtatcta
     2521 tgttaagaac tgaaccagct gacggtctag cactacaagc aggtaaaata atatttgaat
     2581 atttacctcg tgcatataaa aatggaaaga atgataaaga agcaagagaa aaaatggcaa
     2641 tggcatctac tatggcagga atgtcttttg caaatgcctt cttaggaata tgtcactctt
     2701 tagcacataa attaggtgca ttccaccatg ttcaacacgg tgttgcaaat gcattattaa
     2761 taaatgaagt aatcaagttc aattgtgcag aagctccaaa taaaatgggt gcattctcac
     2821 aatacagata tcctgattgc atacaaagat atgctgaatt tgcttctttt gcaggtatta
     2881 aaggaagtac agaccaagaa aaagtagata atcttataaa agcaatagat gaactaaagg
     2941 caaaagtagg tcttcctaag actataaaag aggctggagt agaagaatct aaattcttag
     3001 aaagattgga tgctatggtt gaacaagctt ttgatgacca atgtacaggt gctaatccta
     3061 gatacccatt gatgagtgaa ttaaaagaga tatatctaaa agtttattat ggtaaataat
     3121 atctattgtg caacataggt tctattgggt ttgtatataa attattagat ttgaatataa
     3181 atagagtttt attagagaca cgtagaagta actataaata atagattata acttaaattc
     3241 taaatggaaa tgataaaaag tcagtaattt aatggtaatt tcgaatatgt gtatttaatc
     3301 aattaacttt aaaattagtc ggaaaatttt tttattacat ataattttaa agcataatat
     3361 ataaaaaact ttatgaaaaa attataaaaa tattcagaaa attctattga ctaatttatt
     3421 tatcatgata taataagttt agaaaagaaa aaaattgggg cctattaagg cccccttact
     3481 attttttgat agatttattt aaaattactt tctcattttg attttttata tttattatag
     3541 agtcagtata atccacttca aagttggcaa aagcagagaa taaatctata tctcttaaca
     3601 aatgaattgt catacattga ttgttcttat ttagccttag aatcaatgct cttacaaatg
     3661 gaaattgtat tctagaatta aataagttag aaccatcagt atctatatct aagctacgat
     3721 aattaatatc tataaatata tctacatcat cattttcttc tcttattatt ctgtagtcaa
     3781 aatccaagtt taaatttaaa ttacctaaaa tcaataacat ttaaatcctc ttttctataa
     3841 atagttttag agtgttaatt taagctctcc atctattata gatataattc tatctgcttt
     3901 tctagcaata ttttcattat gtgttattat tataagggtt tggttatatt tttttataga
     3961 actttttagt aaatccatga cttcttctgt agttttactg tccaaatttc ctgttggttc
     4021 atctgctagg attatacttg gttttgcaga taaagcacgt gcaatagcaa ctctttgctg
     4081 ttgaccacca gataattggt tgggaagatg atttttcctt tcattaagac ccaataaatt
     4141 tataaggtca tcaatatatt ctttatctat ttttttattg tctaacttta gtggtaattc
     4201 tatattttcc tttacattta atacaggaat taagttatac tgttgaaaga caaatcctac
     4261 cttttgtctt ctatatcttg ctaattcttt atctgaaaga caacatatat ctttaccatc
     4321 tacctttata gtaccattac ttggtctttc aagaccacct actaaatgta gaaatgtact
     4381 tttcccactt ccacttggtc caattatagc aacaaattcg cctttattta tttttaaatt
     4441 tgcattcttt agtgcatgta cagatgtttc ctttttccca taaatcttac ctaaattaat
     4501 agtttccaat ataggcataa ttatccctcc tattctgtaa tgtttatagc ttcaactata
     4561 ctaattttat ttatctttct tgatggaata tatgtagcta gaataccaac aattatattt
     4621 gctaaaataa ctaggatata tattttgtaa tctattgaaa atcccaatcc atatgataca
     4681 aagccctgca tataatacat tttaaattgt attataattc ctgacacgat agtaattata
     4741 cttgagagta caccatataa tattccttca taaagtatca tatttttaaa gcctctctca
     4801 gagataccaa tagctcttaa cattccaaat tcactagttc ttgaagttaa gttataactt
     4861 atgttgttta ttatgttaag tacactgatt atgaacatta ttgctactat accataacta
     4921 taaatcatag ttctagatgt catcttatca gaattttctt tttctaccat catatctact
     4981 gttgtagttc caggaacttc acttccaatt ttacctaaaa ggttatttat ttttttcttg
     5041 tttgcatttt cattaaggtc tgcataaact aagttatact tatctacacc agttaatttt
     5101 tttaggtatt cgctactcgt tattacatct acaccatcat ctccagagta cataccatta
     5161 tctgcaaaag gataacttat aatagctcca actttaaatt catattctaa gtattcataa
     5221 ttatctttta gactttcata aagccctgta tccatcccac cctttggata tttgactttt
     5281 atagtatcac ctacttttat atctacagct ggtgttcctt caccaggagt aaaagtcttt
     5341 gtgtatttct gtgttttaga aatttctggt acatatacaa tagctaaatt ttcttttttc
     5401 atttttttca agtttatatt tccacttact aagtattttt ctaatgactt tatcatctca
     5461 tcattataac cttttagctt ttgctttagt gtatatttgc cagtagtctt attttttatg
     5521 aacagaggat ttagcttata ataaggagtt gaatttatag cttcgtagta atctaatcta
     5581 tttattttat ttacctctat tcttgtgttt aagatactag cagttttgac tttgtcaatt
     5641 ccatctatat tttttatttt atctatctga ttatcattaa acccttttat atctttaaaa
     5701 gaagtattgt taccaatttc attgagataa acatttgata gaataaaatc tgcattcata
     5761 tatgtttgtt cttgagtaga tttgttttgc acatctaaaa aagaatattt ataattgtct
     5821 tttatgacga ttatacctcc tatactcata gataatacaa ttataatgaa ccccttttta
     5881 tttctaaaga tgtttttcgc agatatagat tttgttactg acatatttaa agttaatttt
     5941 gatattaaat aattaacttt ggttacattt ttatcagctc caaaagtttt tctgatagca
     6001 tcaataggag aaattcttct tatttttaag tatgtaaaga aacttataat tagtattatt
     6061 agtattgtac atgcaattga taagagaatt attttatctg gaataacaaa aggtgtaaca
     6121 tttatatttc cttcaaattg aatatttcct acagacctat taaatatttg tgcacctccc
     6181 atacctatac atatacctat aggcattgct attagagcaa gtatgaaaag ttcataaagc
     6241 ataagtttga aaatcctaaa gttggtggaa ccaatagctc ttaagacgcc atattcctgt
     6301 actctttggt atattgatat aacatagata ctatatatta caataccagc aaatagactc
     6361 atagcaattg caacgtattt acttgaatta tctaaagtac cgtttttcat agtagattct
     6421 ataagcattg agtttcttcc tatacttttc tcatctaaca tagcattttt tattatattg
     6481 tctatgtttg tatttatatc actgccttca ttaaattcaa tataagttgt aaaatcattc
     6541 agttctgatt cattgagatt agaaaaaatt tcacattgtc cctttttttt ctctataaat
     6601 ctatcttcaa ggattccaac aactttaaaa gtttctggat tttctttttg atataattta
     6661 aaagtaaggt tttgatttat ctctggtttt aagcccatac tatttaaaat ccatttctcg
     6721 actaccacct cattgctagc ttttggaaat cgacctttta taagtttaga atgactagtc
     6781 aaataatttt tatttgcata tagtatgttt ataggtaatt tttcattaac atctgtagac
     6841 gcatagtaag atgttatacc cagattttta atatctttgc tagattttat ggactttaat
     6901 tgatttttct ttatattttt aaagtataca tggtcagttc caagctctct tttcaatcta
     6961 tctacatctg cttgctgagc acttcttgag agggtaccaa taccaacaac gagtgatgta
     7021 gcaagaatta tactcacaac aattgctaaa gacctagctt tataactttt tatatacctt
     7081 agtactattt tcaaattatc tttcatattt accacttcct ttcttaacta aatcaattat
     7141 atatttatct aaaatatata gcaaagtaag tattttcaag ttttaagtag ttaattgtac
     7201 aaattcatct gtatttttac aag
//
LOCUS       DAEMGG010000160         7141 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEMGG010000160 DAEMGG010000000
VERSION     DAEMGG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.161"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(138..1388)
                     /gene="cme"
                     /locus_tag="KPK24_003399"
     CDS             complement(138..1388)
                     /gene="cme"
                     /locus_tag="KPK24_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG2301036.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(1474..2220)
                     /locus_tag="KPK24_003400"
     CDS             complement(1474..2220)
                     /locus_tag="KPK24_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2301037.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSSPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(2354..3376)
                     /locus_tag="KPK24_003401"
     CDS             complement(2354..3376)
                     /locus_tag="KPK24_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG2301038.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(3376..5796)
                     /locus_tag="KPK24_003402"
     CDS             complement(3376..5796)
                     /locus_tag="KPK24_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2301039.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSLKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(5882..6802)
                     /locus_tag="KPK24_003403"
     CDS             complement(5882..6802)
                     /locus_tag="KPK24_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2301040.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         2358 a         1159 c         1028 g         2596 t
ORIGIN      
        1 agataaacct atttttcaat gttgaaatga taaaatatat ttgatttcat ataaaaaatt
       61 ttcttcataa attaaaaagt ccattttaag ccccttcatt tttaaccaga taaatcgagt
      121 taaactattt aaatatatta ctcggattca tttgacttag atattccttg cttgtaaaat
      181 tgttttgaat aggggatagc caaagcaaaa aataaaagta taatccctgc tatgattatt
      241 aaattattaa tagaaattac atctgccatt ggtccgaaaa tcatcatacc aagaggaata
      301 aaaccagtat atataataat aggtaagcta agaactctac ctagcatttg tggtgctaca
      361 tgctcttgca acatagtcat agctgatgct tgaatgattg gaattgaaaa attcgtaaaa
      421 aacataaata taaaaaattg ccaagcttgt gttacaagac ctagaataat tgaaaaaata
      481 gcataggata atattgctag aaaaaaggtc ttatttctat ttttaaaacc accaaaaact
      541 cctataatca atccacctaa aaccatacct aaaaatccaa tcatttcaat aatagacagg
      601 tatatataac ccttgccaaa tgttcgttca ataagaagtc ctataagaaa tcctgatgga
      661 acacaaagaa gcatgaatgc tccataagta aaaagcactt ttctaatgaa cttatctgaa
      721 aaacagtact ttatccctga taccatatct gcaaaaaatg agactttttc agtttgtttt
      781 ggtatatcat gtttaggaat tttgatgaat attaataata caatagcaat aattgcagta
      841 aatacatcaa ttaaaagaac ttgatttatt ttattaccaa atccaagtat tgcagcagct
      901 acaataggaa ctataaattg tactatagac tgtagactac tatttatacc attaaaacgc
      961 attaaatgtt cttctggtac aagttgagga atcattgcgt ttacagcagg tgtttgtaca
     1021 ccacttccta gtgaacgtat aacagatatt actgatatgg ctattaattc acttgttgtg
     1081 ccatttgatg acatcataaa tagaattaga actaatgtag atatagcaat tgttgaatct
     1141 gatacaatta tgagtacctt tcgattgtag tagtctgccc atacaccagc aaaaaaagaa
     1201 attatcattt gaggtataaa ggagcataca gttaaaactg ttacccatac acctgatgat
     1261 gtctgaagtg tgacatacca tatcattgcc atttgtacaa gtgatgagcc aaataaggaa
     1321 atatattgac ttgctaaaaa aagaatgact tttttcttcc atgtagaatt agtattttta
     1381 ttttccatcc tattcctcct ttgatttgtg tgttgaattt ttcttgctct taaggataac
     1441 acacaaatca aagtctggag ttagagtgga gggctaggaa aattgttttt tcataatttg
     1501 aagatgagat ttcatggttt ttgcattatg ttcagcttct ttcaatgaag ttatcagttt
     1561 gtcacacact gagataattt cgtcatcatc ttttggagtg tcaaggacag ttttaatatt
     1621 agtattagga ctagaagata aagcatttaa cattcgtaaa atagctgaaa gagaatagtt
     1681 tgcacaacga agtgcgctta taattttaag tcgttttata tcttcatcag tatatacacg
     1741 ataaccattt tgttttcttt ttacttttaa caaaccattc atttcccaat ttcttaaagt
     1801 atctattgta acgtttaagt gttctgcagt ttcttttcta gttaaaaaga gtttatttgt
     1861 ttctggagta tttccattta aaatctgtgc aacaatagta agtgcttcct cggcattaga
     1921 tttttctatt tctaattgtt gcaagtattg atttacaaga ctaatagcct tatcaaagtc
     1981 cctattcgct gatgttctta caatctcaag ggcatctttt cttaatcccc tttgtaccac
     2041 ttcaattttc aaggctgtac gagcaaatct aaattgttct atatgaaaat cattaaagat
     2101 acgataacca ttggtattac gcttaggttt aggtattaag tttagttctt catataaacg
     2161 tactgtatta ggatgtacac caattaaatc tgctacctct gatgtcgtgt aagtgtccat
     2221 tagtattcaa ctccttttag atatatagta ttagtcataa taacaccaaa caaaagtctg
     2281 gtgttataat ggagggtttg gctatctaag agtaattagt ttatgaaaga gtaattagtt
     2341 tatgaaagag taattaatct attatagaag taagtatata tttttcaatg aactcccatc
     2401 tatcacgagg tttaaacact attttagatg gtatggctat tactaagtca tattgtggaa
     2461 tacaacaaat agcatttcct ccagttccca ttgctgaata ccacaaaaca ccatcttttt
     2521 tacatagcca ccacagataa ccgtattcat tactattcat tttaactgat tcttcaatcc
     2581 acgatttaga tataatacgt ttattattcc aaataccatc atttaaatac agaaaaccaa
     2641 atcgcaacat atctctagca ctcaatgtaa gtccccagcc acctatagaa taaccctgtc
     2701 tatcttttgc ccaaccctta agattttttc caaagaaatc ttcaaaacca aaatctttca
     2761 tatcataatc tggtataact ttcataccaa taggtttaaa taagtactca ttagcaaatt
     2821 cacgagcaga ttttccagta gtttttgtta taatagctga aagtaggtgc gttccagctg
     2881 aagaatattt gaaagtacca atttcgcctt tttgaccaag ttgattcaag gtatatttta
     2941 tccaatcttt ttgtttgcaa agtctttcta aagactcagt catattttta aaagggtatg
     3001 gtgcagtcat tgtaagtagg tgacgtatag ttactgcttt tttacatatt tcagaaggac
     3061 tacaagtata ttcaggaaaa aaatcaacaa ccttttggtc aacatctttt atatatcctt
     3121 tatctattgc aatacctatt aaggctgata taatactttt tgtaactgat gcaacatgaa
     3181 aagtatcatc agcattatat ccattatagt acttttcaaa gacaattttt ccttttcttg
     3241 caataattat accatttata ttgctatatt gtgagattat taaaggttca agttgtgaaa
     3301 gcttttctga actcatacca acacttgaag ggtctacttt ttcaaaaatt aagtctaagc
     3361 aattgttttt attcattaaa gatacctccc tgttatagat tttggatttt cttttagagt
     3421 ttttggagaa ccttctgcaa ttatataacc tcctttgtca ccaccttctg gaccaagctc
     3481 aacaatccag tcacaaacat ttaatacatc tatatcatgt tcaacaacaa ttacagaatt
     3541 tccattttca accaattcat ctaataattg aatcaactta gaagtatcat atagacttag
     3601 accagtagtt ggctcatcca atacataaag gatattacca tttcgttttt taccaatttc
     3661 ttttgcaagt ttgattcttt gggattcacc accacttaaa gtcggtgtag gttggcctaa
     3721 ttttatatac cccataccta tttgttctaa tacatttaag tgtgaaacaa tatttggaat
     3781 atctttgaag aaatgtacag attcagaaac actcatattt aaaatatcat gtatattttt
     3841 atttttatat ttaatagata aagattcatc attaaatctt tttccctgac attcactgca
     3901 tatatggtct atacttagat tatttcccaa ccagaccttt tcatatccac taccactaca
     3961 tttaggacaa gcccctttgg agttaaatga gaaatgacca gctgtaaatc cttgttctaa
     4021 cgattctggc tgacctgcaa atagtgtacg tatcttatcc catataccaa tataagaagc
     4081 aggagttgag ttcatatttt tgcctatagg agattgagat atctctgaat atccagaaat
     4141 atgtttagta ccaatcagtc tatctgcaat agtttcaaca actgcataat cgtcttcctc
     4201 acctatttca ctctctgaag aatttatttg attattgata gtatggttat taaattggct
     4261 tctaagaaga ggaagtaggg tgtctgatat aagtgaactc ttgccactac ctgattttcc
     4321 agatattcca accatagcac ccaaaggtaa tgatacagtt acatttttaa gattatttgt
     4381 atttgcattt tgaatagtta tacaaggtat tttatcagta tgtgtaatgt ttttattgct
     4441 tgttctatta ggcattggat attttccaga tagatactgt ccagtaagtg atttgtcgga
     4501 ttgaagtaga ccttctaaat ctccttgata tactaattga ccaccttcaa caccagcttt
     4561 tggaccaata tcaataatat gttctgccat ttcaattgtg tttttatcat gctcaactac
     4621 tatgaccgta ttgcctaaat ctttaagttt ctttagtgat tttaagatgt ctattttttc
     4681 tgattcatgc aatccagctg ttggttcatc aagcacataa attaatgagt ccatctctga
     4741 atcgagatgt gaatttaaaa atagtctttg catttcccca ccactcaaag ttgacatttc
     4801 tcggtataag gatagatgac ctaatcgtga ttctattaaa tgttgagttt tggataaaat
     4861 atcatttaca agatttgtac caaatgtgtt taaattacct tgatttatta agagtttaag
     4921 gaaatcatca atttctaaaa tcgtcatttt tccaagttct ccaatatgtt ttccgtttaa
     4981 aagtactcta cgtgcttctt caccaatttt aaaaccattg caatctgaac aggcagtcat
     5041 agtataaact ccatttaaat cttcgcccct tttgtaacga ttgtgaaata cctttgataa
     5101 agaggaactt tttttaccat tttcataatg accatagagt acttcatttc ttatttcttc
     5161 aggtagttgt aagaattgta tgtctaaata atcattgaat tttttagaaa atactctcaa
     5221 atatccaggt gtaatctttg ctctatctaa gacttgtttt agggtatcat gcttatcaag
     5281 gactaatttt tcaatattaa tctcaaaata tgcaccatgt ccagagcact tcatacacat
     5341 tccatctgaa gagttgtatg agaaataact tggtcttaat cgttcttcct catatccaca
     5401 attattgcaa attagattat cgcttacagg agtatcacac atagtacaca ttatttgacc
     5461 ttccactgaa tacaatagtg taagcatgtt gagaatattg gttcttgtgc ctactgttga
     5521 acgaggattg ctctggcgaa taatattttg ttgaacagct atagtgggag ctaaaccttg
     5581 aatattatca aacttatctt cactatcaat acctgcaagt atacctaaag attgcagata
     5641 ttgtttacgc ccttcctcaa atacaatgtc aaacataagg ctagacttac ctgagccact
     5701 gacacctgtt gcaacaacaa gcttatcttt tggaatagaa acatctatat ttttaagatt
     5761 gtgtatcttt gctccattaa tttttatttc tttcattggt tcacctcatt ttatcaattt
     5821 tatcaaaccg taaaataggg atatattaat ccttaataca agaatagatt aggtgaggtt
     5881 attaaaaagg ttctataggt aaatatatat cgaccaagtg tctattttga gggtgattgt
     5941 gtgggttgtt aagatatact tcaaaaggga aggaggcacg tagcttataa ttaccatttg
     6001 atagccattc attatacata aaatcccacg catcactata ttcagatttt gaaatatcaa
     6061 aatgtcccac aagatatttt ccagaaggta ttatcatatt gccaagattg tcattctctt
     6121 tgacaacaat gttatctgga atggtcatgc aaatactagt ccttaattga tgctcctgtg
     6181 taaattgtgg attatcatga taaatggcta atatttgtgt gttttcaagt ccaagtagat
     6241 tctgctcact tgcatatgtg aataaccgtt ctaaaagttt tgggaaagta ctggttagat
     6301 taccatatga accagtatat ctagtgtaaa tagtattcat attatcaagt tctagaattt
     6361 caatttcacc tttaacctct ctagtattct tacatttagg ttttgatatg cttctattgt
     6421 actgagaaat tttatatggt tctttgcaat tcttgctata attgtttcta tattttatag
     6481 ggcttatttt atagtaattt ttaaatgctc tcgagaaaac tgctgaatca gtaaagccga
     6541 agtagtgtgc aatatcagta acagtcatgt cagttctatg aataagaaaa gcagttgcat
     6601 tttctagttt tagcctattt acatattgtg ccaaagtttc tttttctatt gctttgaaga
     6661 ttctatgaaa atgaaatttt gaaaaacctg ctatattaga aagttcatca agagaaagag
     6721 gttcatgtaa atgttcttct atatagtctt gcactttgca aatacgattt agatattctt
     6781 ttttactttt agcatttgtc atatatagcc ctccttattg tgtaaaatgt gtatcaatta
     6841 atttatagca atatagtaat aaaattttat gtttttgtaa tttatgataa ataagatagc
     6901 gtttaatttt ttataattga taaaagatat aagatgcctt atatttgtct ttgcatataa
     6961 gtatagtctt cgtaataatt agtataccat agtgatttat ttttaattgt gtatttatat
     7021 aaatacacaa cttatgatat ggacgaatta ttatgcaaat aagctgtaat taaataaatt
     7081 gcaatataaa ccagttgaaa cataagttag ggaaatatta tcaaagttat tacttagcat
     7141 a
//
LOCUS       DAEMGG010000161         7014 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEMGG010000161 DAEMGG010000000
VERSION     DAEMGG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.162"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..661)
                     /locus_tag="KPK24_003404"
     CDS             complement(<1..661)
                     /locus_tag="KPK24_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG2301041.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KN"
     gene            complement(672..2075)
                     /locus_tag="KPK24_003405"
     CDS             complement(672..2075)
                     /locus_tag="KPK24_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2301042.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(2079..2804)
                     /locus_tag="KPK24_003406"
     CDS             complement(2079..2804)
                     /locus_tag="KPK24_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2301043.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLFAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(2916..3689)
                     /locus_tag="KPK24_003407"
     CDS             complement(2916..3689)
                     /locus_tag="KPK24_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2301044.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(3695..4582)
                     /locus_tag="KPK24_003408"
     CDS             complement(3695..4582)
                     /locus_tag="KPK24_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2301045.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRAGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(4596..5618)
                     /locus_tag="KPK24_003409"
     CDS             complement(4596..5618)
                     /locus_tag="KPK24_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301046.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALN
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            6193..6369
                     /locus_tag="KPK24_003410"
     CDS             6193..6369
                     /locus_tag="KPK24_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301047.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         2440 a         1100 c          632 g         2842 t
ORIGIN      
        1 aattttttaa actataatct ccatttgaca ttgttgtaaa tataaatata cacattaaaa
       61 cataacaaat actcaaactg aactttttac ctctacacaa ataaaaaata atcccaagta
      121 taatccaaat tggtccacct tctactaaaa atggtactgg taccaaaatc ataaataaag
      181 caataactct gttagtgact ttaaaaagta aagctactgt aaatattcct aaaattatag
      241 gaatgattat catcaataat cctataataa aatatttaat ttctttctct tttctaaatt
      301 tctttatgaa ctctattgct tgtaaataaa tcgctattaa aaataatgtt gaaaaaatgt
      361 tatttataat tatagctcct tctggatgtg aaaatacatt attaattatt tgatttccta
      421 tagccataat aacagaccaa atatacaatc tacttatata tttttttctg ttactagtgt
      481 gataaaatcc ttcaacagcc atgaatataa acagtggtgc tgatattctg cctacccaat
      541 gaaaccaaat tggtatttgc attgtttgtg gcataaatgc tgctatatgg tcaaatgtca
      601 taaatattaa tgctaaaatt ttaatagtaa acccacttat acctttttta aaaaaatcca
      661 tatcatatct cctaattttg ttttatctct ataataattt ttgttcctat tccaatttca
      721 ctacttacat aaattttacc tttatgaatc tctattattt ctttagaaat agccatacca
      781 agacctgaac ctttatgcat ctcacctgta ttagtacctc tataatattt tttaaaaata
      841 tgtttcaaat cacttttgct tattccaatt ccattatctt ttattataat ttctatatta
      901 tcttttttta caatttctac acttatcaaa gttccttcgc tattatgaac tattgaatta
      961 aatataagat tggttatagc ccttctgaat aagattgaat ctacatatac ttcaattaaa
     1021 tcttcattac tttcaaattc aatattccta tttttatatc tattatcatt taaaatatca
     1081 attattatat ctctgactaa actgactaaa tttatttttt tcttgtccag aactatagta
     1141 ttatttttta atcgagtaga taaatttaaa tcatcaacta aatcttttat atacttagac
     1201 ttattatata taatttctgt atactcttgt atttcatctt cttcaaactc ataatctttg
     1261 tcattaatta tctctgcata cccttgtata gatgctagag gtgtctttat atcatgtgtt
     1321 atatttgcca gccattcctc tctcaactct tcattttctt ttctctcaac ttcatttact
     1381 cttaaagtat cagctaacat atttatattt ttaaaaactt cttcatatat acctctgtcc
     1441 ttaagatata aactataatt tccattagcc atagtctcaa tactccatag tacgcttgaa
     1501 attggttttg taagttttct actaaataaa tatcctacac ctaaagctac cacgctatca
     1561 attactagtg ttataatgat aactttattt aaagtttttt taatgttatt ttgagaatag
     1621 gttaaaatta ttcttgatat atctctacta ggtataccta ctattatgtt taaatgtttt
     1681 ttgtttaaat atttttctag tacaaaatta acatactttg tttctatata cttgtaatta
     1741 ttcaccattt gaaagggtgt atattttttt ggagtatcct ttggcacatt tactccataa
     1801 acttcctctc ctaaatcatt taaaacctgt atccaggaat ttgatttttc taatttttca
     1861 gctccaatct tagatagttt aaattctcca tctttttcat ataaatcttt ttcaaaagaa
     1921 cgtgcaaatt cttcaggatt atttccagaa tctacaactt taaaaaagct attagttgaa
     1981 atcacataca atatagatat gatatttata attactactg atatagctac aaacacaatt
     2041 gtaaatataa aatttctagt aatcttccac ttcatattct aatcttcctt tactgctaat
     2101 ttatatccaa gaccttttat tgtcagaata tattcaggtt ttgaaggatt atcctctaat
     2161 ttttccctaa tttttcttat atgaaccatt atagtattgt caaatccaac ataatcttct
     2221 ccccacactt tatcccatag tttttcttta cttataattt gattttgatt atgtgcaaat
     2281 aaagatagga tttttagttc ctttgcaaac agtcctatag attttccatc ttttataagt
     2341 tctgccctac tttcatttaa tatatatggg ccaaatgata taattttttc ttcattatta
     2401 tttagatttt tactttcgtt ttgagcatca ctaaaataac ttaatcttct caaatgcgct
     2461 ttaactctaa aagccacttc cttagggcta aaaggttttg taacataatc atctcctcca
     2521 atcgctagtc caagtaactt atctaattct tcggttttag ctgataaaaa tattattgga
     2581 gaattggagg tttttcttat ttctttacaa atatcataac cttcgccatc tggtaacata
     2641 atatccaata caatcaaatc aggattaata cttttaaata attctaatcc ttctttttta
     2701 gtttttgcag tatatatatt attaaggcct tcttttttta ggacagtttc taaaagcttc
     2761 aatatttcta cttcatcatc tattattaaa acacgatttt ccataaacaa cctcctgtaa
     2821 taatttacat atatattagc atatatttta tctaaaattc atttatctta tttttaatta
     2881 aactaaaata taaataaaat tactattatt tttagttaaa accttttgct tgatgtaaaa
     2941 ttttcatatg ttattttagt taacactatc attaatataa ttgagattac tatagaaaaa
     3001 actatcaaaa tacttggtac attatactta accatttgtt ggctcatcat aatccaattt
     3061 ggagtcactg acataaattt tatagcttta tctggtataa aacctacact aaagtatgga
     3121 attgccatta tcaatccacc tagtataaat ggaattacag ccgatttaaa tatagatgaa
     3181 agtgttaagc ctatttgtgc aaatataaaa gaagcaaaaa tagtagtcaa tataacaatt
     3241 atatacattt gtattactgt aagatttaaa ttactatttc caaagtacca taagttatga
     3301 atcgattcat ttaaattttt aaaatcaaaa aaactaaaac ctctaattcc tactaacaat
     3361 ataaaagaaa tattaataat agataaaatt aatccattag ctactagttt tgataatact
     3421 actgattttt ttcccttttt agttgtattt attaaacctg tcatattaga atcatattct
     3481 ttaatgtaat ttccagaagt tattataaac attatcaaac ctattagagg tattgagcct
     3541 tctatcttgt aaaatatatc tgcataagta tctaaaacat caaactttct aatcagaaaa
     3601 aatccaaaaa atattaaaaa cagccaaatt atccaaacac tctttgattt taatattttt
     3661 atcatttcag ctttaactaa atacatcatt tctactacac ctcccttgca tcttctaaat
     3721 caaaataaaa catgtataca tcttcgaatt ttgcttgaac tggttgtata ttatcctcgc
     3781 ttggaatatc ctctgaaaca attctaagta ctattgaatt aatatcactt tgaatactta
     3841 caaccttata tttagattgt atatcatcaa cttcacattc atttttaact tgaacattat
     3901 atactttatt ttccatgtga cttaatatgt ctttgtgtgt tcctttcatt aggagtttgc
     3961 catctttaat cattattgtt tcctttgcaa ctgattctat atcggaaatt atatgagtag
     4021 aaagtattat tatcttatct ctagacattt ttgctattaa attcctaaat ctagctcttt
     4081 cttgtgggtc aagacctgct gttggctcat caaggactat tatcttaggg tcgtttagta
     4141 aaacttgtgc tatcccagct ctcctcttca tacctcctga aaattttcct atagacttat
     4201 ttctcacatc ataaagacct actaaatcta ataattctcc tatcttacta tttaaatatt
     4261 ttccccttaa accttttaaa gctccaatat actgtaaaaa ttcttttgct gtaaaatttt
     4321 tataaactcc aaattcttgt ggcaaatatc ctaaaatatt tctataatca gcatccattt
     4381 taaatatatc ctcaccatta taaagtatct gaccttcatc aggttttatc aacgttgtta
     4441 tttgtttcat taatgtagat tttcctgctc catttggtcc tagaagacca taaactccat
     4501 tttctagagt tactgtaatc ttgtcgtttg ctactttttt accatatatc tttgaaagat
     4561 tttctatttc taatttattc atcttaactc cccttttatc tacttatata atctgatttt
     4621 attactaaaa aacataatga catagcacct atcaatattg ccatcaaaag tcctattgct
     4681 atattgacaa tgtctatatt cactttaaat atcataatat cacctagata atttcctatt
     4741 attttattaa aaccaaatat caaatcataa taatttacat ttgatacaac tgaaagtaat
     4801 tttttaggta atatatggat taccgacatt atcttagtta tagatataat aaatataaaa
     4861 aaacttattg aagatattga agacttactt ataaaagacc caaatatggc tatcatggcc
     4921 catccttcaa acatcaaaag ctctatgcct atatttaata ttacatactt tgttatactt
     4981 aaattagttt tcaatgcaac tacattgtct actattctaa aagattctaa gtctccatta
     5041 acaggtactc cttgttgcat acaagttata ataaatattc ccattaaata aacagaataa
     5101 attactaatg gaactaataa agcaacaaat attttagaat ttaatgcttt tttcttctct
     5161 tttgatgata gtatgatatc ttttaccgaa gtaacgactt catctacgta aatattagat
     5221 ataattatag atataatgat taacattcct acatttataa gtgaatttgt aactctatag
     5281 ttaaatactt tccaaaaact aatatcctta tattcattca cacttagatt atcaagtttt
     5341 ttctttgaca ttaattcaat ttttttagaa aattcacctg tatctgtttg atttgaaaac
     5401 tgctttaaaa cattaatctt attttgaaat ttctcattac ctatgtcgat accacttctc
     5461 gtatcttcta tacgtccttt ttctttatct atataataac tttctgtttc taaaacaggc
     5521 gtaataaaga tactaatcat tagtattaac actaatatgc ctagagatat aaacatactc
     5581 ttagatttag ccaatttttt tatttcccaa tttaccaata tatttctcct cctaaatcat
     5641 tttaattaca catatatatt aaaacttaaa tcttatataa atataatact aaccttaaat
     5701 aaagcttaaa ttattttata atttataggt gtgtttatta ttggagttac atttattatt
     5761 tttctgggaa attatacatt ttcaattatt tttattattt ttgatatttt tattgattgg
     5821 aaattttatt gttgttcaaa ttgtagttat tctttaaatt taaagatgga gttttttaag
     5881 aatagaacct actgtcctta ttatggaaag attattaaat agttgcatat aactttattt
     5941 atcttgaatc ttatttttaa aaacaattat aattttagaa tattttctat tctcaaacaa
     6001 gcatgttgaa taatgaagca tcttctagta ttatatactt gtagaataaa actaaattgg
     6061 taaaactggg aatatttaac cttaaggagt taagttatag aaactagtac taaaaaattt
     6121 tataaattat gttaattgac taatagaatg cgacttttta tttttactaa attttttagg
     6181 gggatggata atatgtttat tggagaaata gacaaatgta cacatatact aactgcttac
     6241 atcagtagtt catatgatta ttgtaatttt ttagatacac agttagatga ttttatatca
     6301 gaatatggag aaacggtagt tgaaacctgc ttacatcaag tcttattatt agtaagtaga
     6361 tacaattaaa actaaatttt tctagaaaaa cctttttaga tttatctact ctaataaata
     6421 tataattttt tgtactttat aagtaactat aacttctgct aaagtaaaga tgtattggga
     6481 gtacatcttt actttcccat tatagaagtt aaaagcatac accataaaat taatcatcca
     6541 atacaaatta tatatttcct atgtctaact taatttacaa gaatcattct atatgcatat
     6601 tctgcaatcc actaaacatg gctctaaacc tttactattt tatacattct ctataaatca
     6661 tacaaatcat atataaaatc cccctaatat gtttaaacca cctataaaat aacaccaata
     6721 tttagaatat tcaatatcaa ctgcataact ggtcaataaa aacgtataac tagacaaatt
     6781 tcaatattta tataaataat acaagaaaaa caagcatttt atttatataa gttttttatt
     6841 ttttgtttat attaaaaaac tatattgatt aatttatttt atttagtaat tataaaaatt
     6901 cgtcttagaa tatattctat atttgtagat tagctttaat atttttaatt tcacaaaaat
     6961 taaactccca atatttttat ccacttttat ctcattaatt aattatatta tttt
//
LOCUS       DAEMGG010000162         7006 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEMGG010000162 DAEMGG010000000
VERSION     DAEMGG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.163"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            387..764
                     /locus_tag="KPK24_003411"
     CDS             387..764
                     /locus_tag="KPK24_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2301048.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            757..1905
                     /locus_tag="KPK24_003412"
     CDS             757..1905
                     /locus_tag="KPK24_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG2301049.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1922..2524
                     /locus_tag="KPK24_003413"
     CDS             1922..2524
                     /locus_tag="KPK24_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG2301050.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2617..3639)
                     /locus_tag="KPK24_003414"
     CDS             complement(2617..3639)
                     /locus_tag="KPK24_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG2301051.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3968..4150)
                     /locus_tag="KPK24_003415"
     CDS             complement(3968..4150)
                     /locus_tag="KPK24_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301052.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4463..4882
                     /locus_tag="KPK24_003416"
     CDS             4463..4882
                     /locus_tag="KPK24_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG2301053.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            5013..6854
                     /locus_tag="KPK24_003417"
     CDS             5013..6854
                     /locus_tag="KPK24_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2301054.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
BASE COUNT         2856 a          753 c          999 g         2398 t
ORIGIN      
        1 ttataattaa tttagatata tttgtaaata atcagtataa aatagatgtt ttaatatcat
       61 aaaggcataa ttagaaggtt attaaaattt attagatatt actctagttt attttaaaaa
      121 aaagtattat aatattcctt gtgtgaaaat ataggaattt ttattaaata gttttaatgt
      181 gtaaataaca ttctacacta ttgtaattaa atatgtaaaa agtaaaatat ttaaatagat
      241 atattttaaa atctaacttt taatattgta aaaaaattag aaatttaaca aaaagtacaa
      301 aaattcacat ttttttcata aagtgtttga ctaaaaaatt cacatgttgt agaattaagg
      361 tagcaggagt tttgagggag gtgtaaataa tgataagaaa aataccacaa gcagaactaa
      421 aagtaatgaa attcatttgg aaagtagatg ttacagtgac atcaaaagat gttattgaag
      481 ctatggaaca aaaatatggc tggaaacaaa ccaccacact gacactttta tctaggctgg
      541 taaaaagagg attcttagat gctcaaaaaa tagacagata tacacattac acaataagag
      601 taaaacacaa agattattta agttttgaga caaaggattt cctaagtaat atacatgatg
      661 actctttaag aagcttaatt tcagcattac atgatgatga aaatataggt aaagataagc
      721 tagattttct agaaagttat tttgataatt taaaggatga ataattatat gctatatgtt
      781 tttgaaaatc ttataaagac aactattgta tgtagtcttg gtatttgtct attattattt
      841 ttgaaaagat atttatttaa gaagtttagt aagagattca attattatat ttggttgata
      901 atagtcttta gaatgttact ttttctattt aattatacaa tagtgtatga agtaaaagaa
      961 cccaaagaaa atgcattagg aaataatatt acacaaattg atatttctac agataataat
     1021 cttatgctgt atgtagctta tttatggtta tttgtaacta tagtcattgc agtttataca
     1081 tttataaagt atacaagatt taaaaatcta gttgttgatg tatcttatga tattgaagat
     1141 aatgatataa actgtatcta caaaaacttg ttaaaagaat taaatataaa aaagaaaatt
     1201 gaattaagag gttctgatga attgataagt ccagcaggta tgggattatt taaatcttac
     1261 atattcttac cagactatcc ttatagtaaa gatgaactaa cttggatttt gaaacatgaa
     1321 ttaatgcatt ttaaaaataa agatatttta ataaagtttt tagttttgtc tgttagaata
     1381 atatattggt ttaatccact tgtatatgta atgagcaata aagtaaatct agattgtgaa
     1441 ttatgttgtg atgaaagtgt tttggtagaa tgttctttaa aagataaaaa agaatatgca
     1501 ctagcactta taaagtcaat taagtttagt aaaaattata atagtaagat tttgacaacc
     1561 gagtttgata agacaaattt ggaaaagaga cttgagagta ttgttaaaaa gaaaggcaaa
     1621 agtggaatac taattgctgt tttagtattt atgacttcta taacatcttt tgttgaagtt
     1681 gatgctcaag ttagaattga tgagggtaat aatggaatga ataacaatat taatgaattt
     1741 ttattgaata agacgactga taaaattata aatatttatt ataaagatgc accaaatgaa
     1801 attaggaaat tttatgaaga taaatgcgaa ttagaaggta aaatacctag agactatgac
     1861 ataatagaaa tggatatgaa agattataat caattaataa aataattata aggaggattt
     1921 tatgttaaca aaattaagaa aaatagtagc tattatgtgt tgcttattgc ttacttttac
     1981 aagtgtggca tttgctgaca caagtaaaga gaaaggagga agtaaagaga ctgttccaga
     2041 gaaaaaccta gattctttta ctctttctgg gtatatatat acatatgaaa atgcgccaca
     2101 agaaataaga gatgaacata aacaaaattg tgaagagata aatatagacc ctaagccaga
     2161 tgatgagata tttgttccag aatcagctaa tctaatgaat gaggacaata gtaaaggtgt
     2221 ttatgcaagt tatactgttt cttataatat aggtgctaaa actataacaa ttatgagtaa
     2281 tgagtatttg actataagta caacaaaagt tatacgaaag ggtaatagcg gaaaagaagt
     2341 aaaagctgca caaatcatgt taactttact tggttataat gtaggtatag atagttcttt
     2401 tggaagtaag acatataatg cagttgtaag tttccaaaaa aaatacggtt tgtctgcaga
     2461 tggaataatt ggtccagcta cttgggataa attgggtaga ctaactgacc ctactctttc
     2521 ataggattaa aatctaaaaa tatattaaat agtgtctcaa tatgattttt caatttaaat
     2581 gagacactat ttaaatataa atgttataaa actattttac aataaattcc atataactct
     2641 accatctata tccttaacat tttgacaacc agtcaaaatc atagtctcac ataactcatt
     2701 cctaaccttc tctataaaaa actccacacc atcaagacca ccgccaaaag aagcagtaac
     2761 aaaaggtctt cccatcaaaa cagcatcagc acccaatcca agcatcttaa ctacatcaac
     2821 accagtccta actccaccat caacaagtat tgttatcttt ccctttacag acttagctat
     2881 atctggaagt acctcacaag taccaggagt ataatcaagt actcttccac catgatttga
     2941 aacaataatg gcactagcac cagactctac agccatcaaa gcatcatcta cagtcataat
     3001 tccttttata ataaaaggta atctagtgga ttcaaccaat tctctaatct catcaattgt
     3061 tttaggagaa aaaggatttt cttgaaattg attattaata agtccacaag catcaaggtc
     3121 tacaccaact gcaaaagcac ctgcttcttc agacaatcta attttatcta ttatctttga
     3181 attattccaa ggctttataa atacaattcc atttccccta ttatccttca atacatctaa
     3241 attatccagt aaaaaagtat ctacattagt atcgccaacc atagcataaa ttccagaatt
     3301 agaacaacct ctaacaacag gctctatgta ttctttttcg gaaactttac cacccatgtt
     3361 caatatagta cctgatacag gtgcagcgaa tataggagaa ctcatttttc taccaaataa
     3421 ttctatggat gtatctggct ttgaaacatt atgtatgact ctcatattta tctttatttt
     3481 ttctagactt tttctatttt caatgaaaga cgaaccactt cctttaccac ccataccagg
     3541 aacatctcca gcacaagcta atccattaca aattttacat actttacatg ttccattaaa
     3601 gttctctcta gctgatttta gtaagtcatt gtaatccata caaaatctct cctttttata
     3661 atattaaaat tgagtattac tatattgaaa atatcttaat tgtaaatata gggatgttat
     3721 tatgatgagc taaactagat aatacatatt agatattttt tattgttgta gatagttaaa
     3781 ttatatcata ttagatagtc aaattttgaa aaaaactaac ttatttaagt atacattttg
     3841 aaaaaattaa aaaagctata tatagatttt taaagaaatc tatatatagc ttttaagttt
     3901 atgtcaatct tttttgttaa tgaataaata atatagaata caatatacaa aaaagtttct
     3961 tgaaatgcta agataatccc catttacaaa gcaacatcat tatactgtgt aaatttgaat
     4021 caacaatgtt ttcaccatat ttcatcataa aatcctctaa ttgtgtgtca ataaatgaat
     4081 aatatccact tatactatcg atgtatgcag tcattaagtg tatacattca tcacttttat
     4141 ctttatacat atatatctcc ccctatatac aatatataaa tttagatacc aaatcgtatc
     4201 atattaaata gttaagtgtg taacatctat gcagttatat atttatatca ttatattaat
     4261 aactaaagtg agttttgaca atagattttg ggaaactgga tgttcaaagt gcaaacttgg
     4321 attttaaaat cattaattaa gatagcgcat aggaatatat gtatacataa cttaattata
     4381 aagattataa acaattactt ttattaactt tatttaaatg gtagaataac ttggagatta
     4441 ataattataa ggaggatata atatgagttt atataagttt atatatgatg ataaggaata
     4501 tttattaaaa gaggagaact gtagtgcttt aataaatgat gaagaaaatc cagttcaagg
     4561 agtaagtatt tcaaaaataa ttgatatact taatgaagca gaagaagttg attttgatgt
     4621 agaatattat caagaagcat gtccattatg tttagaagga gtaaaagaaa agaaaaaatt
     4681 ctttcctttt ttagaatatc acttttatat tttcagtaaa gatggagaat atgtaataag
     4741 caatatatct gttgattata aaggactatc ttttaataaa ctatcaaggg caaataaagt
     4801 tgataatagt tatattgtaa gtgtgataat ttgcgaaaat tgtcaggatt atatagttca
     4861 gatagaaaat tgtatagttt agtgtaaagt atgtcttaat ttaagagaaa tttctaaatt
     4921 gagacatcaa cttttttcaa aaatagtatg gaaaattact aaatttatga gataatgtaa
     4981 gtcggaggtg agttacaata tatagtaaat atttggaaga gatagatgat acaattttag
     5041 attcaatgtt taaactaacc ttgaatgaaa gattattgac taataacgat tttattgaaa
     5101 ataaaattga tgtagtaaag aatttaattg attgctataa aactgaacaa aatagtttaa
     5161 tgttattaaa atcaaatttt ttgctagctt tattatatgg tattcaaggg tttagtgaaa
     5221 aaatgaaaga gtatattttg atttcctgta aatatattga taaatgctta cccaaagatt
     5281 ataaattttt agcacgattt tattcacaaa tagctatttt aagtttaaaa aatgaagatg
     5341 taaataaggc aaatttatat atggataaat ttaatcttat ttgtgatgaa aataattttc
     5401 taattgaaga aattattttt aaatcacaac ttatttacat taaggcaagt aagtgtatag
     5461 aatcttctgt aataattaaa gagatagaaa cgttgtatat aaagataaaa gaaattaacg
     5521 attttaattg taaaaaaata tattttttta ttattggtaa aatatatttt ttgtttttgg
     5581 atgatgctct aattgctaag gtaaattttt taaaagcaag aaaatatgca gatttactaa
     5641 aggatatgca agtatcttca ctatgtaata taaagatagg tgagtgtgaa tgttcgtttg
     5701 aaaattatga atgttccatg gaatatttca atgaagtaat aagcaataaa aagtatagaa
     5761 atgtaaatat aattcaaaaa tatagagcat caaacaacat aactaaaata ttaataaaga
     5821 ctagaaatta tcctgtcgca ataaataatt tagcaaaatc tgaagtatat ctagaaaaaa
     5881 ttaaaaatca tagcttaaaa gaagtagaga agtttgattt atttataagt ttagccatgt
     5941 attatgcaaa aagtaatgaa aaatcttttg aacaatcaat atgctattta gatagagcta
     6001 aaaacatatt aaatatgata aaaaatatag aagaatatat aatatatgat ttagaaataa
     6061 tttatcacca ggcaaagata tattatatct ttggatatta tgataatgcc ttaaacacaa
     6121 ataaaaaact ccttgaaaaa tctaaaaatg ctaaaaatta taattatgtg aagttagcat
     6181 acaagagtat atatttgtgt tttgaaaaga ttcaaaatta tgaaatgagt atgaaatact
     6241 ttaaaatgta ttatgagtta aaaatgatgt atataaaagc tcgaaataga aattatatag
     6301 attccttaaa ttatagacat gagtatatag aaaaagaaat aaataacatg agaaaagtaa
     6361 aacttgattt agataaaaaa aagtatgttg accatttgac aagtgcatat aatagaactt
     6421 ttttgaacga ttttttagaa aaacaagaag ttagtgaata ttgcacagca tttatgatag
     6481 atgtagatta ctttaaagaa tacaatgata cttatggaca ctataatgga gatattgctc
     6541 ttaaaaatat aactataata ataaaaaaat atctaaaaaa agacatgctt atgataaggt
     6601 atggtggaga agaatttctg atattatctg tatgtaaaga ttataaaaaa agtaaaatat
     6661 ttggaagaaa attatgtagg attgtaaaga aatttttaaa caataatttg acagttagta
     6721 ttggcattga tacatgtaaa aatagtagta tagatataaa agaaataatt gaaaatgctg
     6781 ataaagcttt atataaagca aaggaaagtg gtagaaatag atgtttgcat tatcatgatt
     6841 ttaagaattt ttaactgttg aaataggatt ggtgatttac ttgaaaagga gtttaataga
     6901 agatttaaag agaaatataa acaagataga atacgatatg ttgactaaat tctatctaaa
     6961 tttgacagat ttaaactaca tcttggatta tcacaaagac ctttta
//
LOCUS       DAEMGG010000163         6991 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEMGG010000163 DAEMGG010000000
VERSION     DAEMGG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.164"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..610
                     /locus_tag="KPK24_003418"
                     /pseudo
     CDS             92..610
                     /locus_tag="KPK24_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1042..1296
                     /locus_tag="KPK24_003419"
     CDS             1042..1296
                     /locus_tag="KPK24_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301055.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1332..1655
                     /locus_tag="KPK24_003420"
     CDS             1332..1655
                     /locus_tag="KPK24_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301056.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIF
                     IDYII"
     gene            1929..2081
                     /locus_tag="KPK24_003421"
     CDS             1929..2081
                     /locus_tag="KPK24_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301057.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2275..3198
                     /locus_tag="KPK24_003422"
     CDS             2275..3198
                     /locus_tag="KPK24_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG2301058.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVKA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3465..4145
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3465..3492
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaa"
     gene            4301..4489
                     /locus_tag="KPK24_003423"
     CDS             4301..4489
                     /locus_tag="KPK24_003423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301059.1"
                     /translation="MNKIATKSRAEYMKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            4609..5423
                     /locus_tag="KPK24_003424"
                     /pseudo
     CDS             4609..5423
                     /locus_tag="KPK24_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5475..5636
                     /locus_tag="KPK24_003425"
     CDS             5475..5636
                     /locus_tag="KPK24_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301060.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5965..6429)
                     /locus_tag="KPK24_003426"
     CDS             complement(5965..6429)
                     /locus_tag="KPK24_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301061.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(6565..6882)
                     /locus_tag="KPK24_003427"
     CDS             complement(6565..6882)
                     /locus_tag="KPK24_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301062.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
BASE COUNT         2866 a          686 c         1159 g         2280 t
ORIGIN      
        1 tatacattaa ttcagctgaa tccaatcaaa atacttaaat aaaagtaatc tgaaaataac
       61 ataattcaaa tcaatttatt caaataaacc tcaatttatc tcaaatctta agaagtcatt
      121 aaacaaactt gctaaattac aaagagaatt atcaagaaaa acaatcggta gtttaaatag
      181 aaataaagca agattaaaag ttgcaagact tcaagaacat atagctaatc aaagaaatga
      241 ttttctacaa aaactatcta ctaaattaat aaaagagaac gatattattt gcatagaaga
      301 tttacaagta aagaatatga ttaggaatcg taaactatct cgctcaattt ctgatgtgtc
      361 atggtcagag tttattcgtc aattagaata caaggctaat tggcatggta gacaaattgt
      421 aaaggtgggt aaattctttg caagttcaca aatatgcaat aaatgtgggt ataaaaacga
      481 ggaagtgaag gacttaaata taagagaatg gatttatcct agttgtaatg aaactcacga
      541 tagagatata aatgcaagta taaatatatt aaaagaagga ttaagactaa taacaattca
      601 aaataaataa cgtatgtaag aaccgtagga actacgggga tagcctgtgg agaattagta
      661 agacatatgt tagtatgcaa aattctgtga agcaggaaac ctgtgacttt agtcatggga
      721 ggttcaggct agtattactc attattgtat tcaaaagcta aagtggaaac ctagtgaata
      781 tatgaattta gaagttaatg agagagcatt agcagccgac tcaatactta ttaaaattga
      841 agatgaagaa gaaacaatga aagaagctga aagagagaaa aagagaggta gaagaaggta
      901 gaatttaaag ttaaaatagt tacattgcta atatatgaaa atgttacaaa tttggaaata
      961 gatggatata tatgttgtat agtataatgt agataatata tcaccaagta aaaaacaaca
     1021 aataaatcta aattatgagg ggtggaaaat aaagatatta aacttacaat atgtatattt
     1081 gcttttgtaa ttggaataat agtgttattt tgctgtatag gtctaggtca atttaatatg
     1141 gattgtatta taaaacaaaa tggtgggtca atagctaccg aacaatacaa catatattta
     1201 gaacaatcta tttcacaata tagaaatttt ggttctatac tggttttact aggaggaata
     1261 ggaatactgt ttgataaaag tgtcagaaaa tactaaaaaa gtatattttt atttaggatg
     1321 tgatgaatga tatggcaaga gatgtattta gtgtaaagat gacaccacta gaagcacaga
     1381 aaatggtaga aaaaaatttg gaagcagatt tagtttctgt tgatatttat aacctagaaa
     1441 ataataagtc tataatagtt acaatctttg aaaaatatta tgcaagaagc aatagtgatg
     1501 caggtttggt agtgatttgt gaaaatacta caggaaaaac acttgttaaa attacctcaa
     1561 ctggtagtgc aattggacta tttcagattg attggggaac aggtaataat ttgattaaaa
     1621 gagtaaaaaa aatatttatt gattatatta tttaattatt ttatgtagat attcttaggt
     1681 tggtatggaa tttgtactaa cctattaagt aatcaataaa actaaataaa catagaaagc
     1741 acttacaaat atgtaggtgc tttttattga aaaaatagaa aataaaataa aaagtaagat
     1801 ataggaaaaa tatgtaagaa ttatatgtta taataattgt agcaagaaga tgtaatctac
     1861 aatttataga gtggagttca tgtattaaaa aattatcctc ccaacgataa gaagggaggt
     1921 gaatatacat ggataatttt ttatttaatg tgttagctag tgtaacagct agttacatag
     1981 tttatttgat tagcaagtta atcaaaaaag taaaaagcca ctctaccgca aagagtgact
     2041 taaagattga tataaatttt aaatatcatc gtaaaaaata actttcagtt atagaacttc
     2101 actctaccgc aaatagattg tagttcttct tgtttttatt ataccacaaa ttagaaaaaa
     2161 tattgcatat aatatttttt atagtcaata aaaatatgaa attttttata cacaatgatg
     2221 atgaatctaa ctagatgaat aaatttaatt gtaaataaga aagtacttac ttaaatggta
     2281 ggtgcttttg ttttgccaaa ttatgctata atattgagtg agatgagtaa tttgtggggg
     2341 aattttataa tgtggatatt agtaccaata ataacaatag tattacttat aatagctgtt
     2401 tcttctatgc aatatatttt agttatgata gcttttttgt tgattatata ttcttttata
     2461 gaaaagaaaa tagttatggg tcttgtgtca gttctttttt tcacttattc aatttatctt
     2521 tgtgcaactt gggaagataa atccttgata gcagataata aagttgagac tgttaaggca
     2581 caaagagaaa ctgtagaaag agaaaaagaa atggaaagaa gaagaataca agaagaagta
     2641 gacaaagaaa gatatattga aaaacatgga atggaaatat cagaaaatga tttaaaagtt
     2701 aagctagagg ctttagtacc tcaagaatat aaggggaaga aatatgaatt aaaagtagga
     2761 aaatttaaaa gatatagtat gtattttgat ttaactgtac aaaatgaaaa atttagtaat
     2821 tctgaagaat gtaaaaaatt tgtaaaagaa attgctaatg atcttaaaaa aattaaaata
     2881 agcaaggcgt actttaagtt tcattcaaaa gatgacggcg gtatatataa ttctgtttat
     2941 attgattatt ttagaaacat acaaaataat gtagataatg ttgaaaattt agaattcaat
     3001 gagtttgaac ttaaaaccga agaagaagag aagcgagaac aagagaaaat agaacaagaa
     3061 aaaaacagtt ataataacta cattcaaaac agagtggttg acccactaga tagaataaag
     3121 aaactaaaag agttgctaga ttcaggagca attacacagg aagaatataa taaaaagaaa
     3181 aaagaattat tagaatagat aatatagtaa gcacttacaa gtatgtaggt gcttttaaat
     3241 ttacaaagta ttccatttta attttatagt ttagatttta tgatataata aaaatataga
     3301 agttttgcag tgtgcgatat ttgttacaaa gtagggctta atacttgaaa tctaagatgt
     3361 tgagggtgcg tgataagtgt tatcaattgc actattgccc gctcactgca attttaagag
     3421 tattgtatat atgtaggtat tggaaatgct aagtttattt tggggtttta gattaactat
     3481 atggaatgta aaactgactc gggagcatat aataagtaat atcaagaata gttttagatt
     3541 aactatatgg aatgtaaaat tcgtaaaata acaattgtca ggaatatgag aagtttgttt
     3601 tagattaact atatggaatg taaaattgga ctcaaaatat aagttaaaaa aatttttaag
     3661 ttttagatta actatatgga atgtaaaaat ttttgacgct atgaagaaat ttactacaaa
     3721 atttgtttta gattaactat atggaatgta aaaacttttt tcatcctccc aaacctcttg
     3781 ttcgctgtcg ttttagatta actatatgga atgtaaaaaa gaaaataagt ttaaaaaaag
     3841 gtttcattat tatatcgttt tagattaact atatggaatg taaaaccaat tatgtacagt
     3901 ttagttactg tgttaagtct tgttttagat taactatatg gaatgtaaaa tccttttata
     3961 aatatcttct gacatgtgta tatgcttgtt ttagattaac tatatggaat gtaaatcggc
     4021 tacaacggta taaattaaat ttagcagggt acattttaga ttaactatat ggaatgtaaa
     4081 ttgctgtgat ttcaataata aaacaattaa taattgtgtt ttagattaac tatgtggatt
     4141 caaaattaaa taaacaaaga aagcacttac aaatatgtag gtgcttttat tctgctcaaa
     4201 attggtcggt tgggtaaaat aattagaaaa agttagtaaa aacctattga ctgtaactcg
     4261 ttacaatatt gttattaatg taacgagtta cagaaaagag gtgaataaaa tagcaactaa
     4321 aagtagggca gagtatatga aaaatcgtcg aaaagataaa agaggtttta gtgtactttt
     4381 agacaaagaa aagttagata aatttgatga agtgttagag gaaaagaatc taaccaagaa
     4441 agaatggcta gaagaaaaaa tcgacgagga actggaacaa aaggaataaa aaataagggt
     4501 cactcccacc gaccaaagtt tgagtaaccc ctatgacgta tactatcgta tatcaattat
     4561 agtatatgtc attccttaaa aaaatcaatt attaaggagt gtaatattat gaaaaatgaa
     4621 ttaatgatgt ttgaaggaaa agagattgaa gtatttgaat ttgaaggaag aattttattc
     4681 aactcaaaag atgtagtaaa ctgtttagac ataaaaaatg ttaatgagaa tataagactt
     4741 atgaatgaaa agcaagttgt aaagttaaga aattcggata tcagtaatac tgacatccga
     4801 aaacttaata atgctggaga aaattttctt actgaaagtg gagtgtataa attaatcttt
     4861 aaatcaagaa aagaagaagc tgagagattc caaggctgga taagtgatga agttttacca
     4921 agcataagac aaactggtgc atacataaat aataatgcaa taccgaaaag ttgagagaaa
     4981 aagcaagtga gattgaaaaa ttacagttgg tttataacag tacatctatg ttaaaagaat
     5041 tgctagatga tgcaggcttt gacaataaat caaaactatt aacagctaaa acattatata
     5101 agaaagcagg aattgattta ccaatagaga taaacgaaga agaacattat tttgatacaa
     5161 aacaaatagc atctaaactg aaaatatatt ctaagagtaa taaaccagct cagatggcag
     5221 tttgtgagat tattaaaaag attgatttag aagatggtga agttaaaggt gtttgggaaa
     5281 ttaatggttc ttggactggt actgtaaata aatatacaaa gagtgtaata gataaggtga
     5341 gaacttggat agaggaaaat aatagaccta ccaagattgc aggtgagaag aagaattatt
     5401 atgtatttta caaaattgag taaatttatc agttgtatta aataatttag tttagttttg
     5461 gggggattaa tacaatgtgt gagaatttac ttgataatat gtatattgaa aagagaaaag
     5521 aagaatatag aattaaactt ttgaaaataa gagaaacaga tatagatata tataataagt
     5581 tagaaagtat agtatataaa ctttctgaga aaaaattaga gaaaaataat aaatagacat
     5641 ataaagcact tggatattct actgtttcaa gtgctttgtt tggtataaaa tggtataatg
     5701 gagatagaga attatattaa cccaaaaatg gagaaaatag agaagatgat ttagttgacg
     5761 ctttatcagt aggagtactt gcaggtaaga cagaatctct agtactatta gttggaaatg
     5821 gaataagtga tactcaaaaa agtttaataa ataaaagaga atttagaaat ataactcaaa
     5881 ttggtggaaa tggaaatgag aaagcattta gtgaagtaga aaacttagtt aaatagatta
     5941 gaaggtagca actagagttg gttgttacct tcttttttta tactttctct ctctaagtat
     6001 tttctttttc atatcttcta atacatctaa tgcagtagta taaaattgta tattatttct
     6061 ataaggtata gctattggaa gaatgccctt gttaactaaa aaaataagaa gtacaataaa
     6121 taaaagtgtc ataatcattc taataaaata attagtgata tctgataaat caaatgttaa
     6181 gttaattgtt gtagaaaata caacagccat aatagatata tacaaagcaa aaatggggtt
     6241 actatttttt tgtaactgtc ctttaagcct agattgctct atttctatat cttgaatact
     6301 ttttatggaa tccatttcac tataatactt ataaactttt aaataatatt ttttatactt
     6361 gtttaaagct tcttcattat ctttaaggtc tttgtcactt aactctgatt taacttcccc
     6421 aaaatccata aaatccctcc tacaatgtta tataatatac tttaatttat agcatattta
     6481 agaacttata tcagcaattt gtcgaacgat tttagctaaa aatagagtat aatgataggc
     6541 ttattattta taagatatta ttttttatat agttatatat ttctttctaa ttagataata
     6601 taatgctgtt gttataatgg ctactaatat tattgctatt attgtaatat ctaatcttct
     6661 tgtaaataag cctattgcta tatactcaag acctgttaca tagcgataaa gtacttctag
     6721 tttaacagtt tttttcttat ctatatttcc atatattgat tctaatttat taaaatattt
     6781 tttactacaa aaagcagttt taggtttaat taaatatatt attgtagttc ctattataac
     6841 tattattcca ataagtataa gattgatatt tccaaaatcc atagcagata cctcctatta
     6901 aatttgcttt tcttaatcaa agtatatcag taacacaagt acaaagagca tctaaaatat
     6961 tacaaatatt aacaggatag ataaaactat a
//
LOCUS       DAEMGG010000164         6980 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEMGG010000164 DAEMGG010000000
VERSION     DAEMGG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.165"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            136..312
                     /locus_tag="KPK24_003428"
     CDS             136..312
                     /locus_tag="KPK24_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301063.1"
                     /translation="MKTLMFILVIAYYITIVIKSITTIVKSITRLLDSISRLKESLHK
                     LRNRPDGNQDDFNE"
     regulatory      complement(529..624)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            833..1210
                     /locus_tag="KPK24_003429"
     CDS             833..1210
                     /locus_tag="KPK24_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2301064.1"
                     /translation="MKEEILVEKLLRAELIVMEYLWKKDSLVTKKEIVKEMRQVYRWH
                     KSTIKILLKRLVDKGYLARDIIKFQSHYKIIIDNKEYYAFKKKVLKSSKSRKIMRSLT
                     TTHKSISKEKLDALEEYYRNLEE"
     gene            1320..1703
                     /locus_tag="KPK24_003430"
     CDS             1320..1703
                     /locus_tag="KPK24_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2301065.1"
                     /translation="MKISKLPEAELKVMRYIWEANDVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNAMVKEKKYLRFETKEFLKNIHKNSISSLISALH
                     SKDDEVDEDKLIELEEHFKNLKEKE"
     gene            1888..2472
                     /locus_tag="KPK24_003431"
                     /pseudo
     CDS             1888..2472
                     /locus_tag="KPK24_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            2498..2911
                     /locus_tag="KPK24_003432"
     CDS             2498..2911
                     /locus_tag="KPK24_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2301066.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            2915..3658
                     /locus_tag="KPK24_003433"
     CDS             2915..3658
                     /locus_tag="KPK24_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG2301067.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            3658..4128
                     /locus_tag="KPK24_003434"
     CDS             3658..4128
                     /locus_tag="KPK24_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2301068.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            4145..4903
                     /locus_tag="KPK24_003435"
     CDS             4145..4903
                     /locus_tag="KPK24_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2301069.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            4887..5729
                     /locus_tag="KPK24_003436"
     CDS             4887..5729
                     /locus_tag="KPK24_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2301070.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(5889..6794)
                     /locus_tag="KPK24_003437"
     CDS             complement(5889..6794)
                     /locus_tag="KPK24_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2301071.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT         2711 a          784 c         1195 g         2290 t
ORIGIN      
        1 aaaaaaatgg agaatagata tttgataatt gtaaaattat gttataatta aaatgcaaga
       61 atatatcttg caagaggcta caatctaagg gcgaaagttt tcggaaatca ccttcgaacg
      121 aaagggggtg agtatatgaa aacacttatg tttatacttg ttatagcgta ttacattact
      181 attgtaataa aatctattac aacaatagta aagtctataa caagattatt agattctata
      241 agtagattaa aagaatcttt acataaacta agaaatcgtc ctgacggcaa tcaagacgat
      301 ttcaatgaat aaatcattac ttaaattatt ttgaatttga aacttcgctc taggctaata
      361 gattgtagtt tctttttttg tttttgatac gaatttacat ctttattata tcgcatttta
      421 agaaaaaata aaactgtgaa tgctagaaat atattttatt ttatctctaa gtatatatat
      481 tattagccac ctaaaaaact ttcataaaaa caaaaagaac aatatctttt tggcaactgg
      541 ctggcataat ttataagaat tttataaatc ttagtcccta tagctttgcg tcactaaatt
      601 tctctagttt tgccgattta gttttattct acaactaaaa taatacaata gaattagtta
      661 ttattcaaca ggatagtttg aaaattgaat aattctatag atatgaaagt tatttttctt
      721 ctaagaaaac aaactaaaac agtagaatat ttggtaaaaa ttacctatag tttttgtctt
      781 aaaaagctct tattattaac ttgaactaga acaatcgaag gagtgacata agttgaaaga
      841 agaaatatta gtagaaaaat tattaagagc agaattaata gtaatggagt atttatggaa
      901 aaaagattct ctagtgacta aaaaagaaat tgtaaaagaa atgagacagg tttatagatg
      961 gcataaaagt acaataaaaa tactactaaa aaggctagtt gacaaaggtt atttggccag
     1021 agatattata aagtttcaat ctcattataa aataataata gataacaaag aatattatgc
     1081 ttttaagaaa aaagtgttaa aatctagcaa aagcagaaaa ataatgcgtt ctcttactac
     1141 tacacataag tctataagta aagaaaaact agacgcatta gaagaatatt atagaaattt
     1201 agaagaataa tttcgagatg gaaaaaattg gatggtaata tttagtgtga ttctcttgtt
     1261 tggaaaactt tattgagata gaatttaaca tatacataat tcgataaagg ggtgtgacta
     1321 tgaaaattag taaattacca gaagcagaat taaaagttat gagatatatt tgggaagcta
     1381 acgatgtatt aacgtctagg gaattagttg ccgcaatgga agaaaaatac gaatggaatg
     1441 aatcaactac attcacagtt ttaaaaaggt tagagcgaag ggagttttta agtacagaga
     1501 aaataggtaa acttacacac tataatgcaa tggtaaaaga gaaaaaatat ctaagatttg
     1561 aaacaaagga atttttgaag aacatacata agaattcaat ttcaagttta atatcagcgt
     1621 tacatagtaa agatgatgaa gtggatgaag ataaattaat tgaactcgaa gaacatttta
     1681 aaaatttaaa agagaaagaa taaataaaga ttattttagg tggcaaatta tttttgccac
     1741 cttttttgcc accatgacag tttaataaaa taaaatacaa ataaagaaat ataaataaaa
     1801 tagaataaag aaattgtttt agataggttg taaataaatg tactcataaa aacataaata
     1861 aatataaata aacataaata aaatataaga atttctgtga gaaatgcact taatgaactt
     1921 gtaaaagaag atatattatg taaaaaacgt ggtaaaggga cttatgtaac aataccagag
     1981 agaatagaag caacatgtgc aggaaatagt tttactaatt cttgtcatcg tattaatgca
     2041 aaaccaagta ccaaaataat ctctgtttta ataaaaaaag cagataagca agtagctgaa
     2101 gctttaagta ttgaactaga agagaaggta atatgtatta aaagacttag attaatagat
     2161 gaagtacctg ttatttttga agttgactat tttagaatag attacatgtt tttattaaaa
     2221 gaagaattag aagataaatc tttaatggag gttatatcta ataatataag cacattacct
     2281 aaacgagtag aaaatatatt tgaagtaaaa cattcaaaca aagaatattc tgaccatctt
     2341 aaatgtgcaa gtaatatgcc actattgaaa gtaagacaat cggtgtatac agaaaataat
     2401 gatgtcttat attacaatga gcaatttata agaagtgata agtacaaata tgcagtatca
     2461 gcagaaatat aatctataat ttagtaggag gatgagtatg aaaaagataa tattagcatc
     2521 tcatggagac ttttcaaaag ggcttttaaa tgcagtaaaa atgatagtag gagatttagc
     2581 agattgtgtc agttcgtatg gtttgtaccc tggacaaagt gcttcagatt ttgctttaga
     2641 attagaaaaa acaatatcag aagataaaga atgtgaatat ataattttat ctgacttata
     2701 tggagcaagt gtatgtacag caatgttaag attaactaat ctacataatg ttaggttatt
     2761 ttctggaatg aatttgaata tggtattaga acttcttaca aggttttctg atgaattaac
     2821 cgttgaagat atagaccaat tagtggaaga atctagaaga ggaatacaaa gcgtgacatt
     2881 acaaattaaa gaagaagaag aggtatttta gtttatgaaa aaaaagggaa tattatcagt
     2941 atttaaaagt aaaaaaccaa ttatagcaat gatacactta aaaggagata ctccagaaga
     3001 tatctttgaa agagctaaaa aagagattac aatctttgaa gaaaatggtg tagatggaat
     3061 aatgttagaa aattactatg gtaattatta tgatttagaa agaatacttg aatatgtatc
     3121 aaaagctaat ttatcaatac catatggagt aaactgttta aatgttgata ctatgggatt
     3181 tgaactggca acaaaatata atgctagtta tatacaggtt gattctgtag taggtcatgt
     3241 taaacctaga gatgaagcta ctttagaaga gttttttaaa ttacaacgtt caaaatgccc
     3301 agcgtattta attggaggcg tacgttttaa atatcagcca gtattatcag aaaatgatgt
     3361 tgaagaagat ttaaaaatag gaatgacaag atgtgatgct atagcagtta cagaaaatgc
     3421 tacaggtcaa gaaacatcaa tggaaaaaat agaactgttt cgtaaaaatt taggagactt
     3481 cccattggtt atagctgctg gagttacatt agaaaatgca aaaaaacagc ttaaattagg
     3541 agatatggca ataataggaa gttactttaa agataattac aaagattttg gggatgttag
     3601 tgtagaacac gtaaaaacat tcatggatga aatcaaaaaa attagggagg aattataatg
     3661 attaaattag taagagtaga ccacagactt atacatggac aagtagcatt tacatggaca
     3721 aagtttttaa gcacagactg tatattgata gcaagtgatg acttattaaa agatgaattg
     3781 agaatggcag gacttaaaat ggctaaacca tctaatgtta agttagtaat gaaaagcata
     3841 gcagattcta taaaagcact taactcaggt gttactgata aatacaactt attgatactt
     3901 tgtgaatctg tagaagatgt ttatagactt gctaaagagg taaaagctat taaatctata
     3961 aaccttggtg gaacaaaatc agatgataat cgtgaaaata tatctaaagc agtgcatgta
     4021 tcaaaagatg atataaagat gattaaagag ttagattcag aaggcgtaaa tgtatttgta
     4081 caattagttc ctgatgatga tgctacaaat gtaatgaaat taatataatt ttagggggat
     4141 gaaaatggaa tttacacaag ttattttgat aactctaatt gcattctttg catatatgca
     4201 tagttttgta ggttctacaa tgcataatag acctatagtt gttgcaccac ttgttggttt
     4261 agcactaggt aatcttcata caggtatagt aataggttca acattagaat tagtatttat
     4321 gggtgcattt cctgttgggg caagtaatcc tcctgatttt gtgtctggtt ctattattgc
     4381 cacagcgttt gtaatattaa cgggtcaaga tgtatcagca gcagttgtac ttgcagttcc
     4441 tatagcaact ctagtattgc ttatagataa cttcttaatg acagtagtac ttacatgggg
     4501 agctcatatt gctgaccgtt atgcagaaga ggggaatatt gaaggcgtag aacgtgtgca
     4561 attattattt ggtataggaa ataaattaat attagcaata atagtaggta taggattttc
     4621 attaggtgtt ccagtaatag aaaaaatact ttcatttata ccatcatatg taactcatgg
     4681 aatggatgtt gcagcaggcg tgattccagc aataggtttt gccatgttag ctagaatgat
     4741 gcttaataag aaaacagtag catttttatt attaggattt atattggttg cttacctaaa
     4801 cataacagta acaggagttg cattgtttgg attagcaata gctttaatat atgttaactt
     4861 tgtaggagag aaggaggtaa tagtagatga caacgagttc taagaagtta gagacaattt
     4921 caccagatag taaaataacg aggaaagact tttggaaatg ttttagaagg tcattgacat
     4981 tagattcatc ttggaactac gaacgtatgc aaaatatagc atatgcatac atgatggcac
     5041 caataatccg tagattatat aaagatgata aagaaaagaa atctaaggct ttaaaaagac
     5101 atttagaatt tatgtcagtt actcctcata taagtactct tttggtaggt atatctggag
     5161 ctatggaaga agaaaatgca aagaataaag aatttgatgc taatagtata aatgctgtta
     5221 aatcaagttt aatgggtcca gtttctggga taggagattc atttttctgg ggaacattaa
     5281 agcttatagc agctggggtt ggtatagctt tagcttctca gggtaacata atgggaccga
     5341 ttttattcct tttaataata aatgttcctc attttattat acgctatata tgtctagata
     5401 aaggatttaa gtatggaact caattcttta aagatgtgag tggttcaagt atagtttcaa
     5461 aagttatgga agcagcttca atgttagggc ttatggtaat tggaggtatg actgcatcta
     5521 atgtaatgtt aaaacttagt gttaatgtag gtagtggaga atgggcagag cctattcaaa
     5581 cttacttaga ccaaataatg ccatgtatgc ttccagctat gatattcggt attatgtatt
     5641 ggttattagg aaaaaaggta aagacaacta cgatattaat ttcagttatg attatttgta
     5701 tagtattggc tgctattgga gtagtataaa taaaaaaatc ccaagttcat ttgaacgagg
     5761 gattttttga ctaaattaaa taaaaaagaa agatggttca taataattta taagcattta
     5821 tgtgatagtt ataattagct tattaattag ttataaactc aaaattaagc actactttat
     5881 taaagttatt atattataat ttcgaaatac tcacataatc tgtttagaaa tttatcttct
     5941 aatggggaaa aagttttctt tttagagtaa atgaagaaaa actttctagt aaaatcaaca
     6001 tcctttattt tataacattt tattttacct gcattgagat aatcgatagc tgaaatgtac
     6061 gatataaagg acactccaag acctaatcta accatttcct taatagcctc atttgattct
     6121 acatgagcga ttacatttaa tttacttact ggaaagttat ttttactaag tgtgtttagt
     6181 attaagtttc ttgtacctga cccttctttt ctcataataa aatttagata agccaattct
     6241 cctatatcga tacaattatt tttattatca attttaaaat ctgaaggagt gataagaaca
     6301 agttcatcat ctaataagtc taggtactct atttgaggat tattgatttt agaaccaact
     6361 aatccaaagc ttattcgttc atttaaaatt tctgatatag catattgaga atcataatgg
     6421 cttatggaaa attttacatc tggataactc atagaaaaac tcttcataaa atcaggtaaa
     6481 atataagttt caggtatgga actacatgct atatctataa taccttctat ttttcctgaa
     6541 tattctttta tatcataaat tgcacgtttg caattattca atatatatat agcatgttca
     6601 taaagaattt caccagattt tgtgagtgta ataactttgt tgcttctatt tataagaaca
     6661 gtttctagct ctctctctaa attctgtatg tgtgcgctta cagtaggttg agttaaaaaa
     6721 agttctcttg cagcctttga aaaactctga tgttttgcaa cagcaacaaa tacttcaagt
     6781 tgtttaaaat ccattattta atcctcctta aattatctaa aactattata acatatttga
     6841 taaattaata gtcaaaggtg atttttaaaa tagaaaacct ttaaatattc atggagtttt
     6901 tgattgttta ggaataattt ggtataatgt tgtaaggtac ttacaataaa aatatcgtaa
     6961 attgaatttt caaaattcaa
//
LOCUS       DAEMGG010000165         6957 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEMGG010000165 DAEMGG010000000
VERSION     DAEMGG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.166"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            195..335
                     /locus_tag="KPK24_003438"
     CDS             195..335
                     /locus_tag="KPK24_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301072.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(332..703)
                     /locus_tag="KPK24_003439"
     CDS             complement(332..703)
                     /locus_tag="KPK24_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301073.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1252..1509
                     /locus_tag="KPK24_003440"
     CDS             1252..1509
                     /locus_tag="KPK24_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301074.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1619..2299)
                     /locus_tag="KPK24_003441"
     CDS             complement(1619..2299)
                     /locus_tag="KPK24_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2301075.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            2559..2858
                     /locus_tag="KPK24_003442"
     CDS             2559..2858
                     /locus_tag="KPK24_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301076.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            3182..4837
                     /gene="ilvD"
                     /locus_tag="KPK24_003443"
     CDS             3182..4837
                     /gene="ilvD"
                     /locus_tag="KPK24_003443"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG2301077.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLNFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            5181..5813
                     /locus_tag="KPK24_003444"
     CDS             5181..5813
                     /locus_tag="KPK24_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2301078.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            5836..6648
                     /locus_tag="KPK24_003445"
     CDS             5836..6648
                     /locus_tag="KPK24_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2301079.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         2746 a          768 c         1086 g         2357 t
ORIGIN      
        1 ttattattac gagtttttta ttgcaactgt agaataaaag tattattata taattaagat
       61 aattatctag ggaaattttg gataatcaaa atatatttta taaaatcaaa atacaattac
      121 ataaacttta ttaaaaattt gttgggaatt tctgactaac attaagaacg taagtttgtt
      181 gggggatggt atatgtggaa aagtcaaaca ctactgttac aaaaaaagaa aaaattgaag
      241 atataggaat gaaattagaa catttattga gtgaagatat tactttttat gaattattac
      301 ttgagatttt gtataaagaa aaagaagaga attaattctc ttctttaagg aatcttttta
      361 aataggcatt atcaatcatt ttttctatag cttctctatc ttctttttca agagaatata
      421 ttttttctaa gagctttttt gttttttcac ttactgataa gttctctatg attaatttat
      481 cttctttgct tatttttgta ttatcattta cgcctaataa ataatctgtt gatgtttcta
      541 atgcttttgc taatctaact attatttcac ttttaggttc tcttaaatta ttttcataac
      601 gtgacaagct agcctcagtt ataccgactt tagtagcaag ttctttttga tttatatcta
      661 attcttttcg tagatttgct attctatctc ctaaactttc catatgtaca tcactcctta
      721 ccgaaattat aagttatttt gataaaaagt aaaataaaat taccaaaaat ataaaaaaaa
      781 aaggttaaaa taataaaata tattgaaaat taccatttgt atatgtataa tatgaattat
      841 acaaatggta atttgatgag aggtggtatg ttgaaataag aaactgaatt aattaataaa
      901 tacatagaaa gttttgaaaa cctttaattg aaatataaac aatatgtaca aaattttttg
      961 caagtaaaat taccaaatgt ataaaataaa tcagtagcat agataatttt atgctttaat
     1021 acattagatt atttaattta gttataatgg tcaaaaatgc aatctacaaa taaaacaaag
     1081 tattatcaaa aaagtattct ttaagaaaat tttttattag agatgtaatt gatataaata
     1141 atcgtattaa aaaataaaat actaagttta aaaatttaaa taatctataa atcaattata
     1201 aaatattatt tcttagaaat taaaatgaat taagtaaatg gaggaattga tatgataata
     1261 attagaagtc aagataaaac taatttaatg cagataaacc aaattaaaat agatggtagt
     1321 caagtttatg cagtatttga aagtaaaatt gatactgtaa aaataggaga ttatgaaaat
     1381 aatacaagag ctatacaagt gttggacaat atacaaaact tcattgaaaa tggaacaaaa
     1441 tatgattata taacatcaaa taaagtcaga tacaatgtta ataaaatatt tcagatgcca
     1501 gctaaatagt gagtgtgcat tttaatttaa taactaaagc ccactttctt ttgtggtatg
     1561 aaagagggct ttacatattt gtaaatatcg aagtacactt gttttatcaa taaattattt
     1621 aataaggtat aatggaccaa ttaaacaata aaaattctcc taaaacagca agtattaaat
     1681 atataatagc ttcatttttg taaagagagg taattttttc gcttatgtca tcatcaattg
     1741 taatagcaat ttcatcttga ataatatttt tatatataga tgggaaaata tcttttttct
     1801 ttataagatt aaataaatta tttacaccta cccatataaa atatcctaaa acagcaccta
     1861 atgtgttcat cattaaatca tcaatatctg tagctcttaa gttaaacaat tgacaaagtt
     1921 caattgctaa tgaaaaacct aatccagtta atgaaacatt gataatattt ctaaaatttt
     1981 tccaaattaa aggtagtaaa aatcctaaag gcataaacat aattatattt aaaatatatg
     2041 ttaagatgcc aactgagtca aaaggtatta gatttatttc atcaattcta aaaagcccac
     2101 tataccttcc tatatcccat atgctaccaa ttccagcttt actaagtgct agatagatat
     2161 ataataaaaa aatatatacc catactaaat gtatcattac attcttttca actttcaaat
     2221 tttttcgttt tataacgact atttggtata ttaagcatgg taatataacg caaataaatg
     2281 aataaaatgt ccatatcatt ttattttgtc ccctttcaaa tttatgtttt tagttttata
     2341 agtactatct ttatattaaa ctgtaagttt taaaaatctt ttaataaaat cttaaaaatt
     2401 tattaattta taaaaatata ttattaagat taaaaaatat atataatcaa agtttctaca
     2461 attatatagt tagggatata aaaattttat cgtatgcata aatattataa atatatggaa
     2521 ataataatag tatattttgg taaagtagga ggaattttat gaaatggatt aaatggtata
     2581 gtattacatg catatgtatt tttatagtag tagcttttta tatgtttata tttcctaata
     2641 aaatagaaac tattgatact tcatctgcat atagctttgt tgaaaagaaa gtaccaaata
     2701 gtgctgtgta tcaaggatac aaaaagaatc cagttgatgg tactactaca atttattaca
     2761 gctatgataa ttcaactcat atagttagat tatcacatcc agaagactat tcaaggaaga
     2821 ttaattggga taaagtttca aacatcagat ttgattaatc tttaatattt ataaaagtct
     2881 atatatctaa gtcaaatata ttattaatct tattcatagt tgagcacttt ctattataga
     2941 agtgcctctt ctgatttata tcataaaata ttactcaaca atttgtaaaa tatattagtt
     3001 tataaaatat attgaaaaga taatattata taatacaatt gtgtaagata ttgataaaaa
     3061 atcgtaataa attttcatta gtatatgcta atattttttt acaattttcg taattaatta
     3121 atagaaaaat atattataat aatattatga aaatttaaaa aagtaagaaa gaggtaatat
     3181 tatgagaagt gatataaaaa aaggaataga aggagctcca aagagagcgt taatgtatgg
     3241 aatgggtctt actaaagaag aaattgaaag accattaata ggtatagtaa atgcacaaaa
     3301 tgaggtgatt cctggtcatc tgcatcttga tgaaatagca gaagctgcaa aaaatggagt
     3361 aagaatgagt ggtggtttgc cattagaatt tcctgctata ggggtatgtg atggtatagc
     3421 tatggggcat gttggaatga attattccct ggcttcaaga gaattaatag cagattcaat
     3481 agaagctatg gctatggcac atggttttga tgctttggta cttataccta attgtgacaa
     3541 gatagtgcca ggtatgttaa tggctgctgc aagacttaat ataccaagta ttgttgtgag
     3601 tggagggcct atgcttccag gtaaaaaaaa tggaaaagta tatgatttta actctgcaat
     3661 ggagggtgtt ggagcatgta aagatggaac agtaagtgaa gaagaattag aagcgttagc
     3721 catgaactct tgtcctggat gtggttcatg ttctggtctt ttcacagcaa atagcatgaa
     3781 ttgtttaaca gaagctttag gcatgggtat accatataat ggtactgctg cgtcacattc
     3841 tggagaaaga aaaaggatag caaaatatgc aggtatgtat gttatggagt tacttaagaa
     3901 cgacataaaa cctagagata ttttaacaat agatgctttt aaaaatgcta tagctgtgga
     3961 tatggcaatg gctggttcta caaatacagt acttcactta cctgcaatag cttatgaatc
     4021 aggaatagag cttaacttaa atttttttga tgaaataagt gaaaaaactc cttgtttaac
     4081 aaaattaagt ccaagtggaa aacatcatat tgaagattta catatggcag gaggaatacc
     4141 agctataatg aacgaacttt caaagataaa tggaataaat ttagattgca aaaccgtaac
     4201 aggcaagact ataggggaaa atataagaaa ttgtgaaata gaaaatgaag aagtaataca
     4261 tacattaaag aatccatata gtaaccaagg tgggcttgca atattgaaag gaaatcttgc
     4321 tctaaatgga gctgttgtaa aaaagtcagc agttgcagaa gaaatgttag ttcatgaagg
     4381 accagcaaga gtttttaatt cagaagaaga agctgtaaat gctatttttg gtaaaaaaat
     4441 aaacaaaggt gatgttatag ttataagata tgaaggtcca aagggtggtc caggaatgaa
     4501 agaaatgcta tctcctacat cagcagttgc aggaatggga cttgataagc atgtagcact
     4561 tcttactgat ggtcgttttt caggagcaac tagaggagca tctataggcc atatttctcc
     4621 agaagctatg gaaggtggtt taatcgggtt agttgaagaa ggagatataa tttctataaa
     4681 cataccagac aaaaaattag agttaaaagt agatgaagtt gaaatagaaa atagaaaatt
     4741 aaaatttaaa cctttagaac cgaaaataaa gcatggatac ttaagtagat atgctaaact
     4801 ggtaacatca gcaaatacag gagcagtttt aaaatagtag actatattta tgtaaattag
     4861 aaaaagttaa gaaaatatta attggatatc ttaggtgtta tagcactaat gaggtatcct
     4921 ttttattttg tatgaaaaac acagcaaaat cttgattatc tttgaaaaca atacatctct
     4981 attataagga atacgagtac agcatagaga tgtatctttt agaccttgtt gaggtaatac
     5041 aactttttaa aagtttgatg tggaatgctt ttcgttccat ctgaactatg aagaaattga
     5101 aagtcagtta attcttgaca taataaatat taaaatgtat aataaaaaac aaacagacag
     5161 tctgttttta ggagggaaat atgaatagag aagaaaaaag taaaaatagt aaagaaaaaa
     5221 ttatccaatc agcattttca ctattttcgt ctaaaggata tgattcaaca tctacacaag
     5281 atattatcaa tttatctggt ctatctagag gtgcaatgta tcatcacttt aaaactaaag
     5341 aagatatact gagaagtgtc acaaaagaac tttactcaca aatgaataat tttttagagc
     5401 atcttgttgc tgatgacacc cttacagcaa atgaaaaaat aatagaattg gttgttcata
     5461 gtgcgaatga ttacacacgt agaaaaatgg tacattgtag ctggttagaa aaaatcccat
     5521 tcgctttaat agaggaagtt cgtaatctca acaatgtagt tgcacccaat attgctaaga
     5581 taattaaaca aggtgttgaa aataaagaat tttcttgtga atatccagag gaattagctg
     5641 aaatgctcgt ttttagtatt gatattttac ttgaccctgt attatttaaa cgtgaataca
     5701 gtgaagtatg taataggttg gattttttat tattcatgtt aaaaaaaatg gatattcctc
     5761 taattgatga gtatggtatt caaaaattta aagatttatt tagacaatta taataggaaa
     5821 agcgaggaat agactatgtt ttataatgca aaaagttgtc aaatgatgat aggaagtaat
     5881 acagtgaatt atattgaatt tggtagtggt aagaagacac taattatttt accaggatta
     5941 ggagatggtt tatttccttt acatggaaaa atacaggcga ttgcttttgc ttttagatat
     6001 aagcaatttg caaaagatta taaggtttat gtatttagta gaaaaaatca aattacagaa
     6061 aaatattcca caagggatat ggctaaagac caagcagata taatgaaaaa acttggaatt
     6121 atgaaagcag aagtaatggg tgtttctcaa ggtggaatga tagctcaata tctggcaatt
     6181 gattatcctg agttagttga gaaacttgtg ttggcagtta cttcttcaaa gcaaaatgat
     6241 actatccaaa atgttatttg tagttggatt gatatggcga agaagcagaa ttacaatgat
     6301 ttaatgattg acacagcaaa gaaatcctat tctgaaagat atttaaaaaa ataccaatta
     6361 tttattcctt ttttaggaaa agtaggtaaa ccaaaggatt ttaaacgatt tattattcaa
     6421 gcgacctctt gcatagaaca taatgccttt tccgaattaa ataaaataac atgtccaacc
     6481 ttaattatag gaggtgctaa tgataaaatt gttggaaata atgcttcttt tcatttagca
     6541 gaaaagatta aaaaaagcga aatatttatc tatgagggat tgggacacgc tacatatgaa
     6601 gaagcacagg attttaatga aagagtttta gagtttttaa ataagtaaac acctttgtta
     6661 aaaatataca tatgaagatt ggaaatttaa tgttaaaaat agaaacatga aaatatgctt
     6721 aactggtatt tttactattc ataaccaatt tttgatacat tatctactat aaatacaaat
     6781 atagcttcaa tgtgattata tattagtgta ttggtaaagc acttatacaa acagaggaat
     6841 tttgtaaatt catattatac ccacatttgt taacttatga aaatataatc aaaattttta
     6901 tgagattttt atataaaaaa tataatgttt gtatacaaaa atattgaaga tatttta
//
LOCUS       DAEMGG010000166         6768 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEMGG010000166 DAEMGG010000000
VERSION     DAEMGG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.167"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            376..1269
                     /locus_tag="KPK24_003446"
     CDS             376..1269
                     /locus_tag="KPK24_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG2301080.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1269..2663
                     /gene="gltA"
                     /locus_tag="KPK24_003447"
     CDS             1269..2663
                     /gene="gltA"
                     /locus_tag="KPK24_003447"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG2301081.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3029..5248
                     /locus_tag="KPK24_003448"
     CDS             3029..5248
                     /locus_tag="KPK24_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2301082.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5395..6141
                     /locus_tag="KPK24_003449"
     CDS             5395..6141
                     /locus_tag="KPK24_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2301083.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6141..>6768
                     /locus_tag="KPK24_003450"
     CDS             6141..>6768
                     /locus_tag="KPK24_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2301084.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICL"
BASE COUNT         2650 a          645 c         1238 g         2235 t
ORIGIN      
        1 agaaattagt gttattatga aaacctaagt taatttatta aataaataat tgaataaaaa
       61 aattttatta tgtatttata aaatttttgt tacaaatttg gcataatata gtatacttta
      121 agtgtgtatt aggttaaatc tataacaaat tagacgtatg atatagactt agtgtttatt
      181 ggcatttaca agaaatttgt taatatcaac tatttgtagt ttgggcagtt ataattatta
      241 taggggggat gtccaataca aaacaatcaa ccaagaacta atattatatt ataaagagtt
      301 atagttttgt gtgtttatgt ttttaattaa atttaaagtg ggaaaaatat atttgttaga
      361 atggagaata gcgagatgag taataaaata gttagtaaga gacaattaac agatagtatt
      421 tatttaatgg aaatagaggc acctagagtt gcaaaatctt ctcaaccagg tcaatttata
      481 ataattaaaa atgatgaaaa aggagagagg attcctctta caatagctga ctatgataga
      541 gaaaaaggaa cagtaactat tgtattccaa acagttggtg cttcaactaa aaagttagca
      601 atgtttgaag aaaacgattt tgtaatggat tttgtagggc cattaggaca agcaagtgaa
      661 tttatacatg aagatataga agaattaaga aataaaaaga tattatttgt agctggtgga
      721 gttggttctg ctccagttta tccacaagtt aaatggttta aagagcatgg attagatgta
      781 gatgtaataa ttggtgcaag aactaaagaa ttaattatat tagaagatga tatgaaaaaa
      841 gtagctaaaa atgtatatgt atcaacagat gatggtacat atggatttaa tggaagagtt
      901 acagacctat taaaagattt agttgataat caaggtaaaa aatacgacca agcaatagta
      961 attggaccaa tgataatgat gaaatttatg tgccaattaa ctaaagaact aaatatacct
     1021 actatagtaa gtttaaatac aataatgata gatggaacag gtatgtgtgg tggttgtaga
     1081 gttagtgttg gaaatgaaac taagtttgca tgtgtagatg gtcctgaatt tgatggtcat
     1141 ttagttgatt ttgatcaagc aatgagaaga cagtctatgt acaagactca agagggaaga
     1201 gctatgctaa aacttgaaga aggcgatagt catcatcata gtaattgtgg atgtggaggt
     1261 aacaaataat ggatgctaaa aaagtaaaag taccagttag agaacaagaa cctgctgtga
     1321 gagcaacaaa ttttgatgaa gtatgcttag ggtataataa agaagaagct atggcagaag
     1381 ctaatagatg tttggcttgt aaaaaaccaa aatgtgtggg tggatgtcca gtaggaatcg
     1441 acataccagg gtttataaca aaaattaaag aagatgatat agaaggtgct gctaaagtaa
     1501 tagctaagag cagttcatta ccagctgttt gtggtagagt atgcccacaa gaaagtcaat
     1561 gtgaaggtgt atgtatactt ggaataaaat cagatgctgt atcaataggt aaattagaaa
     1621 gatttgtagc agattggtca aaagaaaatg acataaattt atctgataca gagcctaaga
     1681 aaaatcaaaa agtagcagta ataggaagtg gtccagcagg tctagcttgt gctggtgatt
     1741 tagctaaaaa aggctatgat gtaacaatct ttgaagcaat gcatgagcca ggtggagttt
     1801 taacttacgg aatacctgag tttagacttc caaaacaagc tgttgttcaa cctgaaatag
     1861 acaatataag aaaattaggt gtaaaaatag aaactaatgt aattgttggt aaaacaatta
     1921 cagtagatga acttatagaa gatgaaggat ttgaagctat attcataggt tcaggagctg
     1981 gtcttccaat gtttatgaat ataccagggg aaaatgcaaa tggagtattt tctgcaaatg
     2041 agtttttaac tagagtaaac ttaatgaagg cttatagaga tgattatgat acacctatta
     2101 gttctggtaa aaaagttgct gtagtaggtg gtggaaatgt tgctatggac gctgctagaa
     2161 ctgcattaag attaggttca gaatcatata tagtttatag aagaagtgaa aaagagcttc
     2221 cagcaagagc tgaagaagtt catcatgcaa aagaagaagg cattatattt aatacattaa
     2281 caaatcctaa ggaaatttta gtagatgaaa atggatatgt taaaggtatg gtttgtataa
     2341 gaatggaatt aggagagcca gatgattctg gaagaagaag acctatagaa atagaaggtt
     2401 ctgaatttgt acttgatgta gacacagtta taatgtcact tggaacaagc ccaaatccac
     2461 taatatcttc tacaacaaag agtcttgata taaataagaa gagatgctta ataacagatg
     2521 aaaatggtca gacttctaaa gagggtgtat ttgcaggtgg agatgctgtt actggagcag
     2581 caacagtaat atctgcaatg ggtgctggta aaacagcagc agcatcaata gatgaatatt
     2641 taaaagcaaa agttaatgct taatttgtaa attaaagaga gggtgtctca aaatgaactt
     2701 tttagttcat gaggcactct ttttgtacaa aatcaaatat gttttaatca tttcaacaat
     2761 gaaaatttca ttcctgtaga aagtctaact tttaggtctt agtacaatct ctattttgat
     2821 gtagccactt ttttagatgg tttaataatt tatcagttat tattgtaata tttgatacta
     2881 agtaacataa ctttgtctaa ctatgataaa atagttatac aataaaggtc ttgtaaggag
     2941 tgaataatgt gtggctaata aaatcagatt tgtcaaatct aacattaaat aatagaaaaa
     3001 taaattgtgt aattaatggt attttaatat gaaaaaaaga gaaaaagtat ttgatgggct
     3061 agtttttaag catatattaa tcttattaat actattagtt ttgattgatt taacaagtac
     3121 catcatatat gcaagtaact ctatggaaat tacttctaga aatatgattg aggcatcaaa
     3181 aagagaactt gaaaactatt tagaagtaaa catatcttta ttaaaagctc ttagtcaaga
     3241 tgatagattt tctaatggtg aaacatcact aattgaaaag ggaaaattat taagtccata
     3301 tcaaaaggaa tataatcttt ttatgattgg tataacagat acaaaaggaa atgcttcaag
     3361 tacatatagg gaaaatgtta actcaataaa agataagcct agttttgaga aagcaattaa
     3421 aacaaagcaa gtagtagttt cagatattga agtcagtaat gtaacaggtg acaaagtatt
     3481 tatcatatat gtaccaattg taaaaaataa tgagatgatt gggaccattt ttgcttcttt
     3541 ttattttcaa gatgtaaata atttaataag tagaagcaat tttgatgatt ctattaaatt
     3601 tctaatgata gataaagatt atactattat ttctcatcca aacaaaaaat atgtaaatga
     3661 taaaagtaag atgcttgatt tagaagggaa tattatagga accactaaaa gtgaaatctt
     3721 aaaaaatata aataaaaaat gtcaaggtga ttttttatct tgggataatt ggagattata
     3781 taatgtaaaa tatacgaaca taaagtggac taattggact ttagtatcaa aatgtaatat
     3841 atttaagaac tttaaaaatc taatagttaa ttttatgata aagttgtatt tttatatagt
     3901 aatatttatg atattgtgga aattaagtaa tgctaaatta atagaacaac ttaaaaaatt
     3961 ggcttattat gattctttaa gtggaataaa aaataaagaa aagtttagaa aagattctat
     4021 gtatatttta aagaattatt atcaagataa tttttactta gtacaattgg atgtcaataa
     4081 atttaaatat ataaatgaga tgtttggata tgctgaaggg aataagatat taattcatat
     4141 atcacaggtt ttaaataata atacaaataa atatgaaatt tgtgctagga tggataatga
     4201 ccattttata ttgttgattg cttgtaacac tgaagatgaa cttttaaata gattatctaa
     4261 aataaataaa gaaatttgta acttaaatac tactaactca tctaagtata aaattgttat
     4321 gtcaagtgga atatataaga ttactaaaaa ggatgatatt aaaaagattg acttgttaat
     4381 tgatagagca aatattgcag caaaaagtaa aaaggaaaaa tatgaacatt catattcatt
     4441 ttttaatgaa gatacaagaa atcgattgta caaggaaaaa cgattggaag ataatatgaa
     4501 taaagcttta gaaaaaggtg aatttatagt atattatcaa cctaagtata gtttagatga
     4561 tgtaaatgaa attgaaggag cagaagcctt gataagatgg aatagtccag aatttgggtt
     4621 tatttcacct atagattttg tacctttatt tgaaaagaat ggttttattg taaatattga
     4681 tatgttcgtg tttgaagaag tatgcaaaac attaaacaag tggataaata aagggtatac
     4741 tcctgtacca atctctgtaa atatgtctag agttcattta tatagggata attttataga
     4801 aaatataact gatttgataa gtaaatacaa tatatctcct gagtttattg agcttgaact
     4861 aacagaaagt gttgtgtttg ataatttaaa tatattgatt gatataatga aaaagattaa
     4921 agagatagga ttccttatat caatggatga ttttggttcg ggatattcat ctttaaatct
     4981 gttaaaagac ctttcttttg atattttaaa attagataga ggatttttaa tcgaaacaac
     5041 agatacaaaa cgtggaaaaa ttattatttc taagattgtg gaaatggcaa aagcgattga
     5101 tattaaagta atctgtgagg gcgtagaaac ttatgaacaa gtagagttct tgaaagagat
     5161 aggttgtgat aaggttcaag gatacttatt tgctaaacct atggtattag atgaatttga
     5221 aaaacactta aactttaaat ttgattaatc attaataatt attagactat aagataccct
     5281 aacaacttac ttacaaatat gtaagttaat tgttagggta tttttatggt taaagtagta
     5341 tattcacgat attatggtat tgtagtaaaa ataaaaaatt atggagaata taaaatgaac
     5401 atattgagta ttagaaatat atcaaaaaca tattgtggaa atattccatt taaagcatta
     5461 gataaggtta gtctaaatat agaaaaggga gaatttgtgt ctgttatggg accatctgga
     5521 agtggtaaat ctactctttt aaatataata tctacagttg atagacagag tgaaggtgag
     5581 gttgtattag atggatatga tgtaagtaaa ttgaaaggag aaaaacttgc tgaatttaga
     5641 aggaaacaac ttggttttgt atttcaggat tttaatttga ttgacacttt aactgttggc
     5701 gaaaatatta tgttgccact tacactagag ggggcaagta tcaaagatat gaatatacaa
     5761 actaaaagta tatctaaatt tttaggaata gataaaattt tagatagaaa aacctatgaa
     5821 atttcaggag gtcaagctca aaggtgtgct attgctagag ctattataaa taaacctgcc
     5881 atacttcttg cagatgagcc aactggaaat cttgattcaa aatctactga tgatgttttg
     5941 aaattattta ctaggataaa caaagaacaa aaggtaacaa cacttatggt aactcatgaa
     6001 gcatatagtg caagttattc agatagagta atttttataa aagatggttg tatatataca
     6061 gaaataaaaa aatctgaaag cagtaattct ttctattctg atatactagc tgttttgtct
     6121 caaataggag gtgtaagata gtggatttta gaaagtttgc ttataataat atagttagaa
     6181 atttcagggc atatttagca tattttttaa gttctgtttt ttctataatg atattttttg
     6241 tttttgcagt gtctatgttc catccaatta taacaaaatc tggaatgcag agtggttcta
     6301 ctgcatttat ggctcttatg acttcagaat taataatatt cggttttagt ctacttttta
     6361 tattgtactc tttaggaaat ttcttaaaat atagattgaa agattttgga gtattgatga
     6421 taattggaat gagtaacaga caactgaaaa aattgatatt aattgaaaat ttgataatag
     6481 gatttttagc agtgatgtta ggaatattac ttggtttatc aatttcaaaa ttgtttttat
     6541 tgtatttaag caaactgttt tatatgaatt taactgaatt ttattttcca gtaaaagcaa
     6601 tgctattgac tattgtatca tttatgattt tatttttgat aacaggacca atgagcttaa
     6661 aattattgag taaaaagagc attttagagc ttttagtagg tacaaaaaag cctaaaaaag
     6721 agataaaatc ttcaaagcta tttggaataa ttggtgtaat atgtctta
//
LOCUS       DAEMGG010000167         6724 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEMGG010000167 DAEMGG010000000
VERSION     DAEMGG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.168"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(392..1288)
                     /locus_tag="KPK24_003451"
     CDS             complement(392..1288)
                     /locus_tag="KPK24_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2301085.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKKNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1429..2856
                     /locus_tag="KPK24_003452"
     CDS             1429..2856
                     /locus_tag="KPK24_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2301086.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIAKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFNWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(3052..5646)
                     /gene="clpB"
                     /locus_tag="KPK24_003453"
     CDS             complement(3052..5646)
                     /gene="clpB"
                     /locus_tag="KPK24_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG2301087.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5683..6279)
                     /locus_tag="KPK24_003454"
     CDS             complement(5683..6279)
                     /locus_tag="KPK24_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301088.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6296..6700)
                     /locus_tag="KPK24_003455"
     CDS             complement(6296..6700)
                     /locus_tag="KPK24_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG2301089.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2139 a         1085 c          702 g         2798 t
ORIGIN      
        1 tttttataaa tatataattt ttttatattt atttgacttt cttttaattc tttaggatgg
       61 atttaaattt tctataaaaa tcatactatt ttagctgttt acttgcttat ttaatagttt
      121 tatttctttg attaaatatc tttcagaatc taagccagat gtctcaaaaa aactgattta
      181 taatataatc attttttctg ctattcttat ttttccaatt ttagtttcat attaaaatat
      241 acttttcata gacactacat tctttcatac tcattttcac atttaactat acaagtagtc
      301 ttactcattt atatcctatg ctttattaat atttattaag aaaaattcaa agatttaaaa
      361 atatatattt tctataaagt atatttatga attatttcat atcttctttt aaaataacat
      421 tactaacttc tctatgttca tttctatact tagttggaga tatattataa ttctttttaa
      481 ataagcgact aaaataattt atgctatcaa agccacatct catagcaatt tctgtcatat
      541 ttaaatctgt ttcctttaaa taatatatag ctttatcaag cctgattcca tttataaaat
      601 cgattgtagt cttacctgtt atttgcttaa atgttctaca aaagtgatgt gcactcaagt
      661 tcacacgact agcaagttct tttaatgtaa ttttatccat atatttttct tctatcaaat
      721 caaagataca ttcaaatttt tctaaagaat actttctttt tataaattca tcttcagaat
      781 atattttatc aatatgactt cttaataaga gtactattaa ttgatagata ttagctttta
      841 tagataactc atatccaact tctttattgt gatattcatc aagtatagtt gtaatacatt
      901 ttaaaatcag agaatcattt tgaattaaat tattaaatac aatatgattt aaagccaatg
      961 gtgctaagta cttggtttgt aacaaatcaa cttgattact aaaaaggaat gtgggttcaa
     1021 tacgaatact atagaatttt aaattatctg ataagctttc taaataatgt aattcattac
     1081 tgtttgctat tacaatattt ccaggcacta ccttaaaatt atccaatcca cattcaagat
     1141 atgctatacc atcaataaaa taatatattt gtagatgctc atgccaatgt cttttaaagt
     1201 ttgctccttt tttaatgtca tcctttaaaa aaatatctat aaagttttct tgacctttcc
     1261 tgttaaaata tgtattttta tccttcatta ttcccccctc atcaaaatag tgctaattat
     1321 aagcaatata gttattgtat tcattatttc atatcgctat tatataaaat gtaactatca
     1381 ttgtaaacat taacataatg cagtcattat aataaggagg taataaaaat gacaaactct
     1441 tttaaaaaag atgataatag aaaaaaaatc acagttttat ttgtagtcat tgccatgact
     1501 tttatggcaa cattagattc tagtattata aatgttgctc tcccagttct tgcaagcaaa
     1561 ctaaatgtat ctctagcatc tattgaatgg gtaatagcaa gttactcaat tattatttgt
     1621 tcaacactat tattttttgg aagacttgga gatattattg ctaaatcaag agtatttcaa
     1681 gtaggtacta ttctctttac atcagcttca ttactatgtg gattaagtaa ttctttaact
     1741 ttacttatag tttgccgctt tattcaagga ataggcgctt ctgcctacat ggcaaacaat
     1801 catggaatta tcacagaact atttccaaaa gaaagcagag gaaaagccct agggatttta
     1861 gttactgcag ttgctattgg aaatatggtt ggaccatctg tgggaggatt tattttgtca
     1921 atatttaatt ggaatgttat attttttata aatattccaa tcggtcttat tgtaatcttt
     1981 ttaaatacaa aatttcttcc taatagtaaa aaatcttcag aaaatatgga taaaactggt
     2041 gctatacttc aatttttagg aacaacatta ttttttagtg ctctaattag tgctcaacaa
     2101 acaggattac taaatccata tatattaatt gcactattat tatcaattat ttttattata
     2161 ttatttttga tattagaaaa aaaacatcct caaccgcttc ttgaccttga aatttttaga
     2221 aattttaaat tttcattgaa tttaatttgt gctcttacat cattcatttg tattgcatca
     2281 tcaagtattt taattccatt ttatttacaa agtactatga aacttccccc tattcaagca
     2341 ggtttattta tgatactatc acctttgatt ttagccattt tttcacctat atttgggaat
     2401 atatcagata aaattaagtc agaaaaaatt attttaattg gtcttctcgt tatgagtttt
     2461 ggattctttc taatgtcaag attgaaagaa agttctgccc ttattttatt tgtaatttat
     2521 atatcgataa tatcaatagg acaagctata tttcaacctg ctaacaatgc tttgataatg
     2581 tcaagctgtt ctagaagtaa gctaggagtt gtaggaagca taaattccct tgtaagaaat
     2641 ttaggacaag ttataggtat tactatttct acaacacttt tatataattt tatgagtatt
     2701 aaagctggat atagggtcaa tgattatgtg attaataatg ataaaatatt tgtatttgga
     2761 atgagaaatg tttatattat tgttacactt gtttgcttaa taggtgcaat attaattggc
     2821 ttttatttat ttaaatataa taaaaatgaa caatagtttt tataattata ttcaaattat
     2881 gattatatta taagaaattt actaaaatac ttcctttgag ttaaatttaa tttggacata
     2941 ttgacttaaa aatgttttat taatttgata aaatatttct tttcctatag tttataaata
     3001 aaaatcagct gagcaaaata tttagtatat tatgctcaac tgattctttt attatttaac
     3061 taaaacttca atattttcat cttttccatc tataactata gtacatccat tatacacatc
     3121 tccagcaata agtttctttg caattatagt ttctaatgta tttcctatat atctcttaag
     3181 aggtcttgct ccatatacag ggtcatatcc ttctctcacc ataacatctt ttgctgaatt
     3241 tgtaacttca atagtaatat ctttttcttt taatctattt tttaaatctt tcataaatat
     3301 atctataatt ttcttgatat tttcttggtc taaaggctta aacattataa tatcatctac
     3361 tctgtttaaa aactcaggtt taaatctatg cttcatttca ttcattacta aattgttagt
     3421 agtctctgtt atatttcctc ctgcttctaa tagataatta cttcctatat tagaagtcat
     3481 tattataaga gtatttttaa agtctactgt cttaccttta ttatcagtaa gtctaccatc
     3541 atctaatatc tgtaagaaca tgttaaatac atcctcatgt gctttttcta tctcatcaaa
     3601 tagtattaca gaataaggag cacgtctaac tgcttctgta agttgaccac cctcttcata
     3661 tcccacgtac cctggaggag gtccaactag tcttgataca gcatgttttt ccatgtattc
     3721 agacatatct attcttatta tattatcttc actatcgaat aaatttctag caagagtttt
     3781 tgcaagttca gttttaccaa cacctgtagg gcctaagaaa ataaatgaac caattggttt
     3841 tctttcatct tttaatcctg ctcttgcacg tattactgca tttgaaactg ctgtaactgc
     3901 ttcatcttgt ccaataactc ttttatgaag ttcatcttct agtttaagta atttttctct
     3961 ttctccttca actagtttag ttactggtat acctgtccac tttgatacaa tgctagatat
     4021 ttcttcttca gtaacttctt cttttaaaag agaattttca gaaccatcat tcatattttc
     4081 ttcgtactgc tttatttgtt cttctaactt aggtatttca ccatatttaa cctctgctgc
     4141 tttgttgaaa tcatattctc tttcatactt ttcagcttta cctttagctt cgtctaattg
     4201 agcctttaag tttttaatat ctaatatttg actcttttct ttttcatatt ttgctgtcat
     4261 ttcatcattt ttagatttta actctgctat ttctttttga atatcatcca atcttgattt
     4321 acttttatca tcattttctt taagtaatgc ttctctttca gtttctaatg taaataactt
     4381 tcttctaaca acatctaatt ctgttggcaa tgaatctatt tcactacgaa tcatagctcc
     4441 agcttcatca attaaatcaa ttgctttatc tggtaaaaat ctatcttgta tatatctatg
     4501 agaaagtttt gctgctgcta caatagcatt atcatgtatt cttactccat gatgtatttc
     4561 aaatctttct tttaaacctc taagtataga tattgtatct tcaacagtag gttcttctgc
     4621 aattacaggc tgaaaacgtc tttcaagagc cttatctttt tctatatact ttctatattc
     4681 atcaaaagta gttgcaccaa tacagttcaa ttcacctctt gcaagcatag gtttaataag
     4741 attacctgca tccattgaac cttctgtttt accagctcca acaatagtat gaatctcatc
     4801 tataaacagt ataatttttc catcagaact ttgaacttct tttaaaactg cctttaatct
     4861 ttcttcaaat tcacctctat attttgcacc agcaattaaa gctcccatat caagtgagaa
     4921 tacaatctta tcttttaaac cttctgggac atctcctctt actattctct ctgctaaccc
     4981 ttctacaatt gcagttttac ctacacctgg ctcaccaatc aatacaggat tattttttgt
     5041 tcttcttgat aaaattctta taatccttct tatttcttca tctctcccaa ttacagggtc
     5101 taatttattt ttcttggcta aatctactaa gtttgttcca tatctagcaa gtgcatcata
     5161 tgtgccttct gggtcttgtg tttctactct ttgactacct ctcacttgtg ataaaatatt
     5221 taaaaagtca ttcttattta tattatattg ttttaatatt ttaccaacag ctgattttga
     5281 ttctgtttcc atcattgcaa gcataacatg ttctacactt atatatgaat ctttaaattc
     5341 tttagaaata ctctctgcct ttataagcac ctcatttatt tttctagtag ctgatactcc
     5401 ttgtgagtca gcaccttctc catatacttt tggaagctta tcaatttcaa agtttacagt
     5461 atttctcaaa gaatcaattg atatattcat tttttcgatt atatttggta tcaatccatc
     5521 ctcttgatta ataagtgctg agaaaagatg aattacatct acttgttggt tatgattttt
     5581 tactgcttcg ttataagcct catttaaact tttttgaact cttaaagtca ttttttctac
     5641 gtccatataa atccctccat tttaatatta tatccaaata aattaaattc tctcatttat
     5701 aattattgat tttctaagct ctaaaagact atcaagtaaa ttctcgtttt tttgtatatc
     5761 gaaactcaat gttaaattaa atgttccatc tgaattaaaa aaatcgaata cttcttttga
     5821 ttttgctatt aaatacaagt cttcatctat aagtcttttt tcacctatta ataaatactg
     5881 acatttatct ataaaactct ttttaaaatc aatagtgtat agattttcta aaatattata
     5941 ttgataaata gcaatattat taaacacttt taatatgttt gaatttacaa aattgtaaat
     6001 ttgattattg ttatacagtg atatttgttc gtatatatat tccctaaata aaggatgaat
     6061 gttttgctta agttttaaag taacttcagc tggtatttca aaataaagct tccagtactt
     6121 agagttattt ttttctaaaa taaaagctac tttgttcaaa ctggtagaac taatctcctt
     6181 tagtgtacaa ttaaaattat agtgtctcaa actagttttt tcattctcaa atacatagtc
     6241 aaaccactgt agaacatttc tatcaagttt taaactcata taaaaacctt cttccttatt
     6301 tactaacctt attaggtctt tttatctgat tatattctgc taataaactt tctttatcag
     6361 ctctactacc tttatatgct atatgtggat ttatcatata ctcaccattt ttcactcttt
     6421 gtaaaaaatc catttttttc agctccgtaa acaatgaaga taaagaagtt tttggatatt
     6481 caaattcaca agataattca tataatgttt ttctaatctt attattttta tccatagagt
     6541 ttattaattt taatggaatt aaatccctct tagtatacat tccttctact actttaatat
     6601 aattcttaaa acaaacattc atatacttac ttttattcat aactaaactt ttgctctctg
     6661 tatattcctg atttaaatcc ttttttaatg tcttatccaa attaaatatc tcttctcact
     6721 ctcc
//
LOCUS       DAEMGG010000168         6701 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEMGG010000168 DAEMGG010000000
VERSION     DAEMGG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.169"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            428..4561
                     /locus_tag="KPK24_003456"
     CDS             428..4561
                     /locus_tag="KPK24_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBG2301090.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISPPIGST
                     NLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDADNGTF
                     GFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSNVIGA
                     WWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVTGYPP
                     PQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFSEGES
                     AIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTNVFFR
                     DLIPNGLEFIPGTVTVDGVSVPGVDPNTGFSLPDIGGGTGVEVTFDVVVESIPNPNPT
                     NNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEELIYTI
                     KVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETRVIII
                     RGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNSAVPG
                     TLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGTLNSG
                     ISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISIIKVAD
                     EDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWNGSLN
                     IGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSADIEV
                     IKTSNMDTAVPEETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVTFQPW
                     NGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDETDIS
                     PLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYSIDNG
                     VTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNNNTST
                     SEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPASILSP
                     EYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPN
                     PDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTLIDNV
                     SDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNIAEVT
                     STTPDSNLNNNISRVQVEIKQFCCNDCGCKDCCCNCCCNDCGCKNCCCNCCKNDFWC"
     gene            5018..5365
                     /locus_tag="KPK24_003457"
     CDS             5018..5365
                     /locus_tag="KPK24_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG2301091.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(5515..6564)
                     /locus_tag="KPK24_003458"
     CDS             complement(5515..6564)
                     /locus_tag="KPK24_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2301092.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
BASE COUNT         2489 a          983 c         1057 g         2172 t
ORIGIN      
        1 tctaaatatc ttaaataatt aagtttaaat tttacgatat tttgtttgat tatttataat
       61 ataaaagcat actcttaatc ttcaaaattt aaaaagagta aaaaataaca aaaaaatcac
      121 tgacatttca ctgacaaatt gtaattttgt tattataaat acattgaaaa tacttgatta
      181 ttgaaaaatg gcataaaata atacatttta tgccatttac tattatttga tagtttttaa
      241 cttatgacat aagatatttt ttaacacata caataaaata taaaaaaatt gatattgcaa
      301 ataaaaaaaa tacatattag atgttataga aaaaaagcgc acttataggc ttaaaatgtc
      361 acataaaaga gttttaaaga ataatatatt atgaacacaa attttgtaat cacaaaggag
      421 tgataatatg gcaacagtat caggacaaat agtttttgat agaaatagaa gtgctaccat
      481 tgattctgga gactcaggaa tagccaatat accagttgta ttacaagata tagcaactgg
      541 agttagactt gtggttctca cagatgcatc aggaaattat gtttttacta atgttccaaa
      601 tggtaactat agaattgtag aagcttttgg aactacagga ggagtaccaa caccagggaa
      661 ttttgcaaca gcgatagtag gtccagttcc aattgcagaa atgccaccta ttagtcctcc
      721 tattggttca acaaacttag atggtacaac acctaacaca ctattaataa cagtagcagg
      781 ttcagatata acaaatcaaa atatattaaa tgggcctgtg atttatacac ctatagagaa
      841 tatattggat tcttgcgtat ctgtatctaa tataaacctt attacagatg cagataacgg
      901 aacattcggt ttttttccac caggtacacc aagtaataca ggacctgaaa tagaacctta
      961 cccaaatgtg acgccagatt ttacgtatgt tgtaccagac ccaactaagt ttacaccttt
     1021 agatggagaa tatacagtac aaaatattat gacaaatgca atgagtaatg ttataggagc
     1081 ttggtggcgt attgctgacc atactacagg aaatgaaaca ggaagaatga tggtggtaaa
     1141 tggttttaat cctggagctg tattttttaa atcaacagta caagttacac ctaacactaa
     1201 ttatttattt agcacttgga ttttaaacct ttttaaggta acaggatatc caccaccaca
     1261 gctaggtgta agaatatttg accaaaataa taatatacta tatcaagcaa cattaggagc
     1321 tcaaattcca gttaatacaa acgcaccaga atggaaagaa gtaggtactg tgattaattc
     1381 tcaaaataat aatcaactta cagtagaatt ttttagtgaa ggagaaagcg ccattggaaa
     1441 tgattatgct atagatgatg tttctttaag agaaatagct gttccaactt ttactccaat
     1501 aaaaacaagt aacacagaaa ctgctaatgt aggtgatata atcacatata cagttacatt
     1561 agataatact tgtgatagcc ctttaactaa tgtatttttt agggatttaa ttccaaatgg
     1621 tttagagttt atacctggaa ctgtaacggt tgatggagta tctgtaccag gagtagaccc
     1681 taatacaggt ttctcacttc cagatattgg aggaggaaca ggggttgaag taacttttga
     1741 tgttgtggtt gaaagtattc ctaacccaaa tcctactaat aatatagcta atatagatta
     1801 ctcctataca cctgtagaag gcggtatacc aaatgatttt tcagtagatt ctaatccagt
     1861 tcctgttgaa gtaatatcag cagatattga agttacaaaa ttatctgaac ctactatagt
     1921 gaatccagga gaggaattaa tatacactat aaaagtagta aataatggac cattcccatc
     1981 tgaaaatgtg gttttaacag atgatgtacc agcttctatt ataaatccag agtattcatt
     2041 agatggagga gtaacttttc aaccatggac tggaagttta aatataggga cattagaagt
     2101 aggagagaca agagttatta taattagggg tattgtaaat ccatcaacag taggaattat
     2161 aacaaataca gctgtagtat cttcaactac agcagaccca aaccttaata ataatacatc
     2221 tacaatagag acagaagtga atttattagc agatatttta gtaatgaaaa cagcagaacc
     2281 taattcagct gttccaggta ctttattgag atatactatt caagtagaaa acttaggacc
     2341 agcaaatgct gaaaatgtaa tattaaatga tgatattcca gcttctatta taaatccaga
     2401 atattcattg gatggaggag caagttttca accatggaat ggaagtctta atataggaac
     2461 tttaaactct ggaatttcat taacagtttt aattcaagga acagttagtt taaattcttc
     2521 agaatatata gtaaatacag caacagtttc ttcaactaca cctgacccag atttaagcaa
     2581 caatatatct accattatta caccagttaa cccacaagca ggtatttcta ttataaaagt
     2641 agctgacgag gatgtagctg ttccaggaga agaatttgta tatacaattg aaatttttaa
     2701 tgaaggacca tctaatgcta cgaatgtagt tttaacagat gatataccag atgttatttt
     2761 aaatccagaa tattcattgg atggaggagc aactttccaa ccatggaatg gaagtcttaa
     2821 tataggtaca gttgcaccag ggcaattaat aagaattatc ataaagggat tagtaagttc
     2881 aactgctaca ggagatatta ctaatacagc agaagtaagt gcagatgtac cagaaccagt
     2941 tacggattca tcaacagtga caactcctat agttccatca gcagatatag aagtaataaa
     3001 aactagcaat atggatacag cagttccaga agaaactttc agttatacta taacagttac
     3061 aaatttagga ccatctgctg cacaaagtat cgttttgaca gatgatatac cagatgttat
     3121 tttaaatcca gagtattcat tggatggagg agtaactttc caaccatgga atggaaatct
     3181 tagtataggc actttggatg ctggagaaat aagaagtatt ataataagag gaacagtgag
     3241 ccagacagca gtaggtacaa ttataaatac agcaacaata agttcaccta cacctgaccc
     3301 aaatccagat aataatactt caacagatga aacagatata agtccattag cagatatttc
     3361 tgtgataaaa ggcaacgaac cagttgctat accaggagga agatttattt atggaattga
     3421 aattgcaaat gcaggaccat cttttgcaga gaacgtaact ttaacagata atataccagc
     3481 ttctatttta aatccagagt actcaataga taatggagtg actttccaac catggaatgg
     3541 aagccttaat ataggtactt tagatgctgg agagataaga aatatcataa taaggggaac
     3601 agtaagtcaa acagcaatag gtacaattat aaatacagca acagtaagtt caactacacc
     3661 tgacccaaat ctaaataaca atacttcaac atcagaagca gaggtaagtt catcagcaga
     3721 tatttctgta gtaaaacgaa gcaatcagac tgttgttgta ccaggagatg tactagacta
     3781 tacaattgag gtgagaaatg caggaccgtc tactgctcag aacgtaactt taacagataa
     3841 tataccagct tctattttaa gtccagaata ttcaatagat aatggagtga cttttcaacc
     3901 atggaatgga agtcttagta taggcacttt agatgctgga gagataagaa atatcataat
     3961 aaggggaata gtaagtcaaa cagcaatagg tacaattata aatacagcaa cagtaagttc
     4021 aactacacct gacccaaatc cagataataa tacttcaaca tcagaaatag atataagttc
     4081 atcagcagat atttctgtaa taaaattagc aaataaaaca gaagcatgtg taggagagca
     4141 aatagatttt gtaatagtag tatcaaatgc aggaccagaa aatgctcaaa atgttacttt
     4201 aatagacaat gtttcagata agcttaagaa agcagtattt tcacttgata gaggtgtaag
     4261 tttccaacca tggactggaa gtcttaatat aggtacacta ccagctggaa ctttaagagt
     4321 gattatatta agaggaatta taaaatcaac atgtcttgat agattaacaa atattgcaga
     4381 agttacttca acaacaccag attcaaacct taacaataat atatcaagag tgcaagtaga
     4441 aataaaacaa ttttgttgta atgattgtgg ttgtaaagat tgttgctgta attgttgttg
     4501 caatgattgt ggttgtaaaa attgttgctg taattgttgt aaaaatgact tttggtgttg
     4561 attttataaa ataattttgt ataccttaaa ttaaactgaa ataaaaaata aattcacata
     4621 tgagtaaaat attttataaa tattaataaa ttttctatct ttgatatgga ccacattcgt
     4681 aatactagtt attaaatgaa gtggtccttt ttaattcatt aaaattattt attctaatta
     4741 aattttaaat gtattaaata attttaattt tttataaaaa atattcatat gttatttata
     4801 ttaaagttta gatatctaaa aatactttta gaatttatta tattatgtta attttttata
     4861 tcttataagg attgtatttt tctaactata aaaatagtat tatacttata tttaaaaata
     4921 aaagaatttt attcctattg tgtaaagtga gaatactatt ttcttatatt ggtataataa
     4981 tagtaaatct ataatataga aaagaggtat ttctagtatg catgaaaata aaaaaaatct
     5041 attagacgga agcttagctc aatataatga tacagcagtt ccaaaaatac cagtatttgc
     5101 tggtaacgat ataacttcag aagtttatta ttttaaacct aatcaagttc ttaatgctca
     5161 tagacaccct aatggtgaac aaatatttgt gttcctaaaa ggagaaggta aaatgaaact
     5221 tggtgagcat gaatgtgatg taaaaaacgg agatactgta tttgtaccaa caggagagtg
     5281 gcatgaaatt actaatggaa gcaatgaaga aatggtagcc gttcaaatta caaaaattaa
     5341 tgctggagca gaatacagag gatagatata taagtacagt tagtacttta atatctattt
     5401 tattatataa actttaatct caaatataaa aagagtcttt taaaaggctc tttttatatt
     5461 tttgttaatt ttcattataa aaacaagaaa atatctagct aaaactaata tattttattt
     5521 aatatttttt atagcattgt ttgcaaactc agtatttacg atatctttat aaggtgcttc
     5581 cttatctagt tctttagctt ctttcataac agtcatcaat ttcttcaaac cttcctcttt
     5641 taatacagga ttttgagacc atgcatctat tgatttatat ctatctacaa ctgttgttaa
     5701 atcttctaaa gatatatctt caaagaaagg ttgcatagcc ttagctactt ctttgctaga
     5761 tgcttgttga atccataatt gacctttata aacagcattt gtaaatcttt gaattaaatc
     5821 tttattgttt ttcatgtatg atctcgtagt agagtatgct gtaaaaggta tttctccaga
     5881 atcttttcct atagatgtaa ctatatatcc actaccttct ttttccattg ctgttgctgt
     5941 aggttcaaat gctgttgtaa agtcaccctc tccacctgca aaagctccag ccattacacc
     6001 aaattgtaca tcagttctta cattaacatt gcctgctttt agattttctc caatagtaag
     6061 acctttttct tttagaacat attcaaaagt cattagtgga actccacctt ttctaccacc
     6121 taatatttcg gttcctttta aatcattgta agagaaatta ggcatttttt tcctagcaac
     6181 tagaaaacta ccatctcttt ttgtcaattg agcaaagttc actgcataat cgtttttgtt
     6241 ttgattatat acatatatag atgcttcagg acccattaaa cctacgtttg cttcacctga
     6301 aatcaaagct gccatagttt tatctgcacc ttgggtattt ataaggtcaa tctttatacc
     6361 ttcatcttca aagaatcctt tcgtaatagc agcatactgt ggagcataaa aaacagagtg
     6421 agtaacttct gctatagtta ctttatccag atttttagac gaagaagatt catctttttt
     6481 gtcattagag ttaaaatcac atccagttag acatattgta cttacaatga tagagcttgc
     6541 tattgcgact atttttttaa acataataaa tacctcccat aagacatacg aatagtatat
     6601 agtattaaat atttattgta gttgttacaa aaatgtatct atacttataa tatttctaca
     6661 aaaacttaat aataattttc agttttatgt ttagtttttt c
//
LOCUS       DAEMGG010000169         6682 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEMGG010000169 DAEMGG010000000
VERSION     DAEMGG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.170"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            225..926
                     /locus_tag="KPK24_003459"
     CDS             225..926
                     /locus_tag="KPK24_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG2301093.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            919..1242
                     /locus_tag="KPK24_003460"
     CDS             919..1242
                     /locus_tag="KPK24_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG2301094.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(1448..3508)
                     /locus_tag="KPK24_003461"
     CDS             complement(1448..3508)
                     /locus_tag="KPK24_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2301095.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3704..4396)
                     /locus_tag="KPK24_003462"
     CDS             complement(3704..4396)
                     /locus_tag="KPK24_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2301096.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            complement(5008..5382)
                     /locus_tag="KPK24_003463"
     CDS             complement(5008..5382)
                     /locus_tag="KPK24_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2301097.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            5601..5834
                     /locus_tag="KPK24_003464"
     CDS             5601..5834
                     /locus_tag="KPK24_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301098.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            5818..6231
                     /locus_tag="KPK24_003465"
     CDS             5818..6231
                     /locus_tag="KPK24_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301099.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            6250..6546
                     /locus_tag="KPK24_003466"
     CDS             6250..6546
                     /locus_tag="KPK24_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301100.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITNEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
BASE COUNT         2268 a          972 c          712 g         2730 t
ORIGIN      
        1 aaattacacc agatagtaat taacttatag agattaatat gtattattac atcataataa
       61 caaatagata aaatttaata accgtttaag attatctatt tgttgtatag tattttatat
      121 attaatccaa atttattttt aattataatt gcaattatac aattaatcta tttttctgtt
      181 ttactaatac tttaatacat ttaatacaaa aggagtgatt tattgtggaa agaactcaac
      241 aattaaaaga aagctttatt gcttcaattc ctatattctt aggttatata cctataggta
      301 tagtaggagg aatactatta caaaaatctg gtctttcacc ttttcaaata gcactaatgg
      361 taactttagt atttggagga agttctcaat tcattgcagc ttcaatgatt tctactggtg
      421 caagtgtaac atctattgtt ttgactacat ttatagttaa cttaagacat tttctaatga
      481 gttctaattt gaacatgtac ataaaaaata aaagtccaaa atttatattg ccattttgtc
      541 atactataac agatgaaact tttgcagtta attatgaaaa atttactgct ggaaattggt
      601 ctgatagaaa tgctatttat cttaatttct tctgcttaat ttcatcagtt ttagctaatt
      661 ttattggagc atttttagga gaaagtatat ctatagacag ttctatttct ggcttcatat
      721 tgacttctat gtttatagct ttaatagttt ctcaaataaa aaataagatt tatattgtag
      781 tatttatatc atcaatcatt atttctgtta ttttatatgc attattcaaa agcaatttag
      841 taataatagt tgcatcaatt ttagcatctt taattggttt ctttatagaa gaatcttact
      901 gtaaaaagga ggaatcttat gaataataat tatatatttt ttattataat tggaatgtac
      961 attgttacat atctaccaag agtgcttcct atgttaattt tttcaaaaaa agagatgcct
     1021 gattttttga aaaaaatcat gaaatttata cctgtagcaa tacttacttc cttaactgct
     1081 aaagatgtat tttttaatgg agatagtcta tatttatcta tagctaaccc taaaattata
     1141 tcttttatga ttgtacttct tgttgcatat aagtttaaat ctattggtat ttctattatt
     1201 acaggagtga tatctatata tttatttgga attatcttgt gatatttgga taatgtttta
     1261 ttgtataagt tattttaaaa ctatgtgtac aattttaatt taagttgtta tattattgat
     1321 tttaaataaa aaacagagtg acttaaagtt actctgtttt tactcttcat tctctactgt
     1381 aaatattcta atatattata ataaaagctt tcgacagtaa aaaaaatata tatacttaaa
     1441 tactatctca ttccatattt gttatttctt cttcatattt ttgaaactct aaatttgctt
     1501 taaataaatc tccatctatt tctatataaa attttccacc ttgtaaagct actaaatctc
     1561 ttgcaatagc aagccctaat ccacttcctt cagtattcct tgattcatca gctcttttaa
     1621 atctttccat aatctcactt gggtcaaaat ttaattcata agaagatata tttttcatag
     1681 ttacctttac attttcacca tagtcatata catcaatata gactctagtt tcttctaagg
     1741 aatattttgc tatattagat aaaagatttt caaatactct atacattctt cttccatctg
     1801 cccatatata caccttatca tttggaacat ttaattttaa atataaatta tttttagata
     1861 atttttcttc aagttcacct atactttgtc ttaatagttg cactaaatca atcttgctaa
     1921 tttgtaattc tatattgcca ctacttactc tactcgcttc aaataaatct tctatcaata
     1981 attttagtct ttttgacttt gactcaagta catttatata atcatttata tactctggtt
     2041 gtattgattc ttctttttta attaaatcta cataatttat tatagaagta agtggagttt
     2101 tcaaatcatg agatacattt gttataaggt cagacttcat tctttcactt tttacttgct
     2161 cttctatagc tctatcaaga ccttctccaa tattatttat attttcagct aaagacgtaa
     2221 aattatcatt cccagtaatt tttatcttat aatctaattc tccctttttt attctttctg
     2281 taccttctat tacgtaacta agatatgaaa gtcttttagt tatatatatt gtaaatccta
     2341 aaaaaataca tatagaaaac agtaatccca tacctatact tcgagtgcta attgccatga
     2401 ttaaagctaa agatattatc actataacaa taactttcct tgctaaagat actttcttta
     2461 tagcgttact gacctccctt acagttttta ttaaccataa aatcactctt actataatac
     2521 ttgtcttaaa tatacacatt ttattttcta aatagctcaa ttgcacattt ataaaaaata
     2581 tattatttga tactatcagt aaagataaag aaactaatgc aattacatca atatgataag
     2641 atacagcaag agcatatata gttaaaattg caattgaaat agatactaat attacattaa
     2701 attccatagg tatcttactg tatattagta aaaatacatt atttcttttt aaaacttcac
     2761 cttttatttt gatatataaa attatagata ataaacatat aattgctaaa cttaaactag
     2821 ctacaaaaaa taatcttact ttttctgtag cttgtttaaa tatttgaaaa ttagtatata
     2881 ctattccatc ttctatagtt ggctcttctg gaaaagatac atatgcctca aaaccttcat
     2941 ctacttcatt tagttccaga ctcagattat tcagttcaaa tttttctcca tttattgttt
     3001 taatatatga gctctttcct atcttattta ttatctctac attacaatag ctcttagttt
     3061 tttgtttaaa atcatataaa ctagcatatg acgtatttgt ataaaatact cctgtgtatg
     3121 tattaataac atacactttt atatttttta gataacttaa atattcttca gaagcttgtt
     3181 cattaatcat gattttatca tactcagaat cactactact taaatctata cttttacctt
     3241 cttgttggaa tttacctttt tctttactag ttaatttacc acctacagat agcttaaact
     3301 tgggtaatag acttgccatt gttccttcgt caaatatatt actatcataa aaatcatcag
     3361 atatatcaaa aatcccactc tttgtagaag ttttttgtat attgtattta ggctcagcaa
     3421 gtcctattgt caccaacaat atagttaatg gaaatgagat agatattatg attaatgaaa
     3481 ctaactttaa tactctatat ttctccaagt tgcccacctc atctacattg tcagtatttt
     3541 atataaaata aagtttaaga aaagcataat ccccatagag taaattaaac tctcagagaa
     3601 aaaattaaaa catattaaaa ttcctaacat ttgtgtaaat gtaaaaatga ctaaaatcaa
     3661 aatgcaatta aatattaaac tcttttcttt tataattttt ccactaaaat ttttcaatct
     3721 tataaccaac tccccacacc acctttaaaa atcttggttc ttttggattt atttcaatct
     3781 tttctcttat tcgtctgata tgtactgcta ctgtattttc tgaattaaaa ctttcctcat
     3841 tccatacttt ttcgtatatc tcatctattg agaaaactcg tcctttattt tctaaaagaa
     3901 gttcaattat tttatactca attggagtca tttttatgtt ttcaccatca attttaactt
     3961 cttttgttag tgtatttaac tccaaagctc cactttttag aatttcttta catgtatctt
     4021 cataattacc taaagttaca tatcgtctta agtttgattt aaccctagct ataagctcta
     4081 atagattaaa tggctttgtt atataatcat cagctccaat atttaatcct attattttat
     4141 ctgtatcttc actttttgct gaaattaaaa ttattggaat atttctttct tctcgtattt
     4201 ttacagttgc tttgattcca tctaatttag gcatcattat atccattagt attagatgaa
     4261 tgtcattctc tgttattatt tccaaagctt tcattccatc atatgcttta tatattttaa
     4321 atccttctga tttaagatat atttctatcg aatcaacaat ttctttatca tcgtctacta
     4381 ctaatatatt gtacatattt tcacttcctc tattgtataa taatacttat ctatattata
     4441 caatatcttt cactcccttt ctcaacaatg actaaattgt tatattactt tggcttacat
     4501 ttttgtaact ttcccttata actatataat aaaatataaa tcttaagaaa atattatcct
     4561 aattcttaaa tatttcttaa gatttaagat aaattgaggt ttatttgaat aaattgattt
     4621 gaattatgtt gttttcagat tactttcatt taagtatttt gattgcattc agctgaattg
     4681 atgtatatta attcttattg aacagttttt attttttggg tggaagaatg gtggaagaat
     4741 tacatttcac taattttctt ccaccattct tccacccaaa tatacttaga aacattcatt
     4801 tcactgcaat aatagctact tatttaaatt ctatttttat acattctatc tactatatac
     4861 tgtacatatt ctattaaaat ttatataact aaaatacaaa cttatttcat tttcttaaca
     4921 tgttcagaat aataaacaaa atgaaggtag aaaaagttat aatttcatac cttcatttta
     4981 tttattatgt atcaatttgt cattttctta tttcaaattc ttaaaatatt cttcgagttc
     5041 acttaattta tcttcatcca cttcgtcgtc ttcaccatgt aacgctgaca ttaaacttga
     5101 aattgaattt ttatgtatgt tcttcaaaaa ttcctttgtt tcaaacctta gatatttttt
     5161 ctcttttact agtatattat aatgtgtaag tttgcctatt ttctctgtac ttaaaaactc
     5221 ccttcgctct aatcttttca aaactgtaaa tgtagttgat tcattccact catatttttc
     5281 ttccattgca gcaactaatt ccctagaagt taataccttg ttagcttcac aaatatatct
     5341 cataactttt aattccgctt ctggtaattt actaattttc ataattcgcg cccctttgcc
     5401 gatttatgta tatgttaaat tctatatcaa taaagtttta caaacaagag aattaaggta
     5461 aataattcca tccaattttt accatattat tcttttaggt tttataatac ttatctaatg
     5521 agattgacat tttactcatt ttaggatatt atgaagctaa cttcacatga agttagcttc
     5581 atatagaaag aggttatgga atgaaaacaa aagtacgaga atttcgaaca aatatgggtc
     5641 taacacagca acaactcgct gatttagtcc atgtatcaaa cagaacaatt atttctattg
     5701 aaaaagagca atatagccct tctcttatgc ttgcctatcg tatggcacaa gtgtttggta
     5761 caacgattga agaactatgt tgtttaaaag aaaataaaga aatggaggat aaaaaacatg
     5821 aaagtaaaga gtaaacgaag ttttatagtg ggtattatag tttgtatgtt atgttgtgct
     5881 agtttagtta tttattgcat tttaaaagat aaaagattcc taatttcatc atttctttta
     5941 attgtaatag caatctttaa tttttgtaat gctttttcaa gaaaaggcat tgttgaagaa
     6001 ttacacgata gtgcagatga aagagacttg tacttaacaa tgaaaacaag tcatatctta
     6061 gttaaaatta tgaattatac attatttact tttacatttc ttttcatcat agcttatagt
     6121 gcatggaaaa atcaatcact tctagttata gctataacac tatgtgtcat tgagattttt
     6181 ttatttgttg catatttact tatcaatata tttttagaga aaaaagagta aatacaaggg
     6241 gtgatataaa tgtattttaa aacttatata ctaaaaagag aagtaaataa caaacaacta
     6301 gagataactc gaagttttga cttagataat ctaatttgta ctgcaacaga gattttgaaa
     6361 gataatgaga ttgatttgat aattactaat gaagtaaata atgttgtttg gagaaatgaa
     6421 aaaagcactc ccccagcaag agaatgctta gttatagcta tagaagtgta ttggtggtac
     6481 acttctattt ttttattata tcatataaat tatgttatca aaaagagcta tttaaatcga
     6541 ttttaaggtg tgttgaataa ggttcttgat agtttatata ttgatgaatt tcaaaaaaat
     6601 aagcactctt tattgtaagg agtgctttgg tgtatgttaa tcttctatct aatacaatta
     6661 taacatgtct tatgttttag ta
//
LOCUS       DAEMGG010000170         6636 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEMGG010000170 DAEMGG010000000
VERSION     DAEMGG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.171"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(156..1046)
                     /gene="metA"
                     /locus_tag="KPK24_003467"
     CDS             complement(156..1046)
                     /gene="metA"
                     /locus_tag="KPK24_003467"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG2301101.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(1076..2341)
                     /locus_tag="KPK24_003468"
     CDS             complement(1076..2341)
                     /locus_tag="KPK24_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG2301102.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(2451..2553)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2607..5264)
                     /locus_tag="KPK24_003469"
     CDS             complement(2607..5264)
                     /locus_tag="KPK24_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG2301103.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFAPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(5392..6135)
                     /gene="yaaA"
                     /locus_tag="KPK24_003470"
     CDS             complement(5392..6135)
                     /gene="yaaA"
                     /locus_tag="KPK24_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG2301104.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(6145..6609)
                     /gene="bcp"
                     /locus_tag="KPK24_003471"
     CDS             complement(6145..6609)
                     /gene="bcp"
                     /locus_tag="KPK24_003471"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG2301105.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
BASE COUNT         2033 a         1222 c          795 g         2586 t
ORIGIN      
        1 tctatctaca tacacctaaa gtgattttct taaaatataa aactactatt ataaggtcaa
       61 attttaaaaa aataaggtat aaagtatcat taagattaac tttataccta taaatttcta
      121 tattttttta gttaacattt atatactctt aaaatttatt gtatcaaata gttttttatc
      181 caatttctaa acaacaattc tgaatgtttt ttccatttta cagttggctc ttctgaaggg
      241 tcatcattaa tatagtaatt ttgaggtata gatattttat ctcctttatt tatatccctc
      301 ttatattctt tatcaagagt gtatttgtca tactcgctat gacccattac gaacacatct
      361 cttgaatttg taattatata gggaccagct tctttagaat gactcacaat gctcaactct
      421 ttattctctt tcaaagcttc aatatttaca gtagtatgtc ttgaatgtgg agcaaaaaat
      481 tcattttcaa atccatctac tattttacta tctttatcta ccttatgctt aaatactcca
      541 aaacattttt gacttagtgg aagttttttt acattgtagt atttgtaaag acttgcttga
      601 gctgcccaac atatatataa tgtagattta gtttttctct tagaataatc cataatttct
      661 tctagttcat tccaataatc aacttcttca aactccattt gttcaactgg agctcctgtt
      721 attataaagc catcaaaata ctctcctttg gcttcatcaa atgttttata aaattcttta
      781 acatattcat tacaacttct tttactttct cttgttgaaa ttttaataaa ttccacagac
      841 acgttgaatc ctgttttatc tatccttctt agtaaatcta attctgtatc tatctttata
      901 ggcattagat ttagtatagc tatcttagtg tctatgtttt cttcatactt taattccttt
      961 ttaaaatctt ctttataaat tacatctatt ccttcatcta ataatttatt aataacaggt
     1021 aatccctttg gtagtattaa agccatatct tttcacttct ctttctctta gaatattaga
     1081 aatttaaagc ttgatttaaa tcagctatta aatcctctac attttctatt ccaacagata
     1141 atcttattag tgaaggcaat actccagatg caattaattg ttcctctgtt aattgtctat
     1201 gagtagttga agctggatgt ataacacaag ttcttacatc tgaaacatgt gtaaccaaag
     1261 atgctatctg taatttttcc acaaattttt tggcattttc taacccacct cttactccaa
     1321 atgataaaac accactacat cctcttgata aatacttttt ggcattctca taatacttgt
     1381 tatcttcaag tcctgggtaa ttaatccaat ctacattttc atgtttttct aaaaatctag
     1441 ctattttaag tgcattttca ctatgtctct ccattctaag atgtagtgtt tcaacattta
     1501 aattagtaag atacgcattg aatgggctta aacaatttcc atagtctcta agcaactgaa
     1561 ctcttgcttt agttgcatat gcggcatttc caaatttttg agtatagctt ataccatgat
     1621 atgttgggtc tggctcaact aattctggaa attttccatt atcccagtta aagtttccac
     1681 catccactat aattcctcca acacttgaag catgcccatc taaatatttt gtggtagaat
     1741 gagtaactat atttgctcca tactttaaag ggttacaaag cactggagat gctaatgaat
     1801 tatcaacaat aaatggaaca ttaattctct tagctacatt tgctattttt tcaaaatcta
     1861 tgacttcaag agttggattt gcaagtgttt ctgcaaacac aaccttagta ttttcctttg
     1921 caagttctac tatctcatct tcacttgaat ctaagtcaaa cgatattaaa tctataccaa
     1981 attttttaag actaggtcct agtaaattga atgttcctcc atatactttt gaagaacaaa
     2041 gtatactatc tcctgattta catatattta aaactgccaa catatttgca gattgccctg
     2101 atgatacagc tacagaagat actccaccct ctagtaaact tattttttct tcaaaagctt
     2161 gaatagtagg attgcttatt cttgaatata tatgtccatc aacttttaaa tcaaataatt
     2221 cagcaagttg gtctatactg ctatatttaa aagttgtact ttgatataaa ggtagtactc
     2281 ttggttctcc attacctggt ttataatttc cttgcacaca tattgtttct ttattataca
     2341 ttcttgtatc ccccttagat tttagtaaaa aaaattaaac tatttaccag atgagagtta
     2401 aaataaataa agcctgagta tataactcag gctctgtata agcttaatta tactcatctc
     2461 tcagctttcg ccgcaggatt tagcaccact gtatataaat atactggttg ccgggtttca
     2521 tagggccagt ccctccacct ctctggataa gatttattta atttttctat gttttatatc
     2581 ttatatattt ttcctaattt tgtcaactat ttttttaatt tttttctttt aataattttc
     2641 ccaacctcta cttgaattac agacattatg gctaaaacaa tcgttataag ccactctaag
     2701 ttatttaaaa ctgttaagtt aaaagcattt ctaaaaacag gtgcaaatag tattactgct
     2761 attaaaaatg cagaagttaa aaatgagatt aaaagaaatg gatttttacc attatttcta
     2821 ttaaacattg aatctgtatt tgaacgctga ctaaatgctc gtaacatctg tgaaaatgct
     2881 aatacacaaa atgccattgt ttgacctaca gaatgatttt ctatattaag accaatttta
     2941 tatgctaaaa tagtcattat tgccacaaat ataccttggg taacaactct ttgtataaga
     3001 tttttttcaa acaaagtggt tgatttcact ggctggtgct tcattatatt tctgcttggt
     3061 gggtcaactc caagagcaag ggctggaagc gtagcagttg caaggtttac ccatagaata
     3121 tgaacagcaa gaagcggagt atcccaattt aataatattg ctacaaacaa cgtaagtatc
     3181 tctgctatat taccaacaag caaaaattgt ataacttttt gtatgtttct atatacacgt
     3241 ctaccttctt ttattgcata agcaattgtt gtaaaactat cgtccattaa aatcatgtca
     3301 gaagaatctt ttgcaacatc tgtaccactt atacccatgg ctataccaat atcagcagac
     3361 tttaaggcag gagagtcatt tacaccatcc ccagtcattg ctggtacttc tccaattctt
     3421 tttagagatt gtataattct aagtttatca aatggggata cacgagcaaa tactgtagta
     3481 tttttaacag cttcatctag ttcatcatca ctcaaattat caagttcatc accagatatc
     3541 acagtatttt cttcactcca tattcctaac tcatgagcga ttgttgtggc agtaacttta
     3601 tggtcacctg ttatcattat ggttcttata cctgcttctt tacaagtact tactgcatca
     3661 attacctcag ttcttggtgg gtcaatcata ccaaccatac ctataaatgt taaatcatat
     3721 tctatatttt cactatcttc ttttggtata gatgaaatct cacgcttagc aaaaccaagt
     3781 acacgcaatg catctttaga catgttaaga cataattcgt gtatattctt tttatccact
     3841 tcggtaatgt ttctttcacc ttttgaagtt aatattttag tacataggtc aagaatttcg
     3901 tctactgctc ctttagtata agcaataatc ttttcatcta tattatgaac agttgtcata
     3961 cattttctat ctgaatcaaa aggctgttca aatacacgtg gatattcttc ttcaagagct
     4021 tcttgatcta ttccaaattt tttggccata aaaattaatg cgccctctgt tgggtcacct
     4081 aatatttcat ctgttttatc tggattaata catgcatcat tacaaagtgc accagcataa
     4141 acaatctctt tatacactaa tggatgtttt tcagatgcag tatctattga tgtcactttt
     4201 ccagattcaa aatctccatt gacagcaata tgtgtaacag tcattttatt taaggttaac
     4261 gtaccagttt tatcacaaca tattactgta gctcctccta atgtttcaac agcaggtaat
     4321 tttctaatta aagcatttct tttagccata cgctgtacgc ctaaagccat tacaatagta
     4381 gctgttgcag gtaatccctc tggaataatt gatatggcaa gggaaattgc aatcataaac
     4441 attggcaaga gaggtctctg ataaaatgca cctattgcaa atataacaac gcatacaata
     4501 atacctatga cacttaatgt ctttccaaca gtattaagtt tacgttttat aggagtatca
     4561 aaatcatctt gtgaatctaa aagatgtgct atgttaccca cttctgtatc cataccagtt
     4621 gctactacaa cacctacacc tctaccatat gtgactattg aagatgtata cgccatattg
     4681 ctacggtctc ctaatacaca ttctttaggt aagattgtat ctgcatcttt ttctgatgga
     4741 acagattctc ctgttaaaga agcctcttga atttttaaac ttgatgtttc tattaaacga
     4801 aggtctgctg gaaccatagc tccatcttct aaaaaaacaa tatctcctac tacaagatta
     4861 cttgctggaa taacattttc ttcaccatca cgcataacac gagctgtagg tgagttcata
     4921 ttttttaatg cttctaatga tgattctgct tttttctctt gaacaattcc aataactgca
     4981 tttactgtta caattattag tattacaaaa gcttctgtaa attcgccaaa tatcaaagat
     5041 aatattgctg ctcctattag tatcataacc atagcatctg taatttgact ccaaagcatt
     5101 tgtaaagttg ttctaggtgg tttttttcta agttcattta agccattctc tttaagccgt
     5161 ttttccgcct cggtatcggt gagcccctcc tctgatgaat ctagactttc taaaatttca
     5221 tgcgttgctc ttgaatgcca aggcatctgt ttgtgatttt ccattttgga agaccactcc
     5281 tttctttttt taatttaaga ataattatat tttaaaatca agttcaattc aatacacact
     5341 aagtatattt tacctaaatt taacaatgcc atttaatatt taacaaaact tctactcttt
     5401 tacaaatatg tattcactat tgttagataa gttttcttta tacttataac cttctaaatt
     5461 aaactttttc agttcttcaa tatctcttat cctatttttt acaatgtatt ttgtcatcat
     5521 acctctagct ttctttgctt gtgtagattc cacttttaat atgccatttt tatttacttt
     5581 aaaagtacat gtaatatatg tatcactatc tttaataaat ttttctatgg atttgctgta
     5641 ttcatttgat gaaagattaa cgatagctgt tttttctttg attagttctt tatatatacg
     5701 atttccccaa tattcgtata aattcttaaa ctcacctact cgtaattttg tctgcatttc
     5761 taatctatat tcatatatac tatcatatgg ttttacaaca ccatacaatc cacttatgat
     5821 tcgaatacag ctatttgcaa actcttcatc tttccttgta aaattttctg cttctatatt
     5881 tttatattgt attccatcat aagttaagat tgcagggttt cctaatctat caaatttaat
     5941 gctttcaaat ctatttttat ttaatacagc taatttttca ttaattttca ttttattttt
     6001 aaaatcttct ataccaaatg tctttatatt ttccactatt tcttttgtgt tatctataaa
     6061 gcaaggcaaa ctcaaatctc tatcaaatac ttctatattc ttcatatttt tagctggtga
     6121 taacattata atcataaatt cttattactc ctgtttttcc aaatattcaa gaatctcttg
     6181 tgcatttgtg tctggttttg ctttttcaaa gactttttca ataattccat tttcatctat
     6241 aatatatgtc gcacgtacaa ctcccaatgc agtttttcca tacatttttt tctctttcca
     6301 tacatcaaaa gcttttatag caactaagtc agggtcagaa agtaaaataa aaggaagttc
     6361 atgcttttct gcaaattttt gatgtgattt tatactatct ttacttatcc caataacttg
     6421 tatatcttcc tttttaaatc catcatatgc atttctaaaa gcacaagctt gacgtgtaca
     6481 tcctggtgta ttatctttag gataaaaata tactactact tttttgcctt taaaatctga
     6541 catacttacc ttatttccat ctttatcttc tagtgtaaat tctggtgctt ttgttcctat
     6601 actcaacata aatatctctc cttttgtttt tattaa
//
LOCUS       DAEMGG010000171         6552 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEMGG010000171 DAEMGG010000000
VERSION     DAEMGG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.172"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(291..1736)
                     /locus_tag="KPK24_003472"
     CDS             complement(291..1736)
                     /locus_tag="KPK24_003472"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG2301106.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(1880..2788)
                     /locus_tag="KPK24_003473"
     CDS             complement(1880..2788)
                     /locus_tag="KPK24_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2301107.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            3045..3869
                     /locus_tag="KPK24_003474"
     CDS             3045..3869
                     /locus_tag="KPK24_003474"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG2301108.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3909..4439
                     /gene="lepB"
                     /locus_tag="KPK24_003475"
     CDS             3909..4439
                     /gene="lepB"
                     /locus_tag="KPK24_003475"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG2301109.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(4521..6302)
                     /locus_tag="KPK24_003476"
     CDS             complement(4521..6302)
                     /locus_tag="KPK24_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG2301110.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         2120 a          971 c          794 g         2667 t
ORIGIN      
        1 aaaatttatt aaattatcta aataacttta tttcagttta cattaaaaat attgcaagta
       61 caatagtttt tgctgaatgt ttaaatttaa ttagtaatct tatcattttt ttatttttat
      121 ttatattatt cctattaaat attatttata tagctatttt attaaattca tggtatagta
      181 agtaaagaag aactcttcac agcggtggag agaagacgct agcccattaa aatggctatc
      241 ttatttataa ataagatagc cgtttttaat tatattttat ctgtacaatt ctatactctt
      301 aagtctacat ctacttttcc taagtcttcc ttataatctt ttatagcagg ttctacaact
      361 tcatttacaa attctacaac ttgttctgga gctctaccta taaagttttt tgggtccatt
      421 atagatagta tttcttcctt gctcatttta aatgaatcat cattcattat aagttcaatt
      481 aagttattat ccttgccttc atgctttact ctataggcag ctttcataga atattctctt
      541 attatttcat gtaattcctg tctatctcca cctcttttta cagcttccat aagtattgtt
      601 tctgtagcca taaaaggaag ttcttcattt acacgtctat ttatcatatt ttcatatact
      661 actaaaccat cagttacatt tatccctatt tcaagtatag catctgctgc cataaatgct
      721 tgaggtattg ccaatctctt attggctgag tcatctaatg ttctctctaa ccattgagtg
      781 gcttgtacca ttgcagggct tatagattca gagattatat acttagataa agatgctatt
      841 ctctcacttc tcattggatt cctcttataa gccattgctg atgaacctat ttgatttttt
      901 tcaaatggct cttctaattc ttttaaactt tgtaaaagtc ttatatcatt agtcatctta
      961 tgcatacttt gagctatccc tgatagtatg cttattactt gataatctaa ctttctagta
     1021 tatgtctgtc cacttactgc ataggaagaa tggtatccca tctttttaca aattttctta
     1081 tctaactctt ttactttttc gtggtctcct tcaaataagc ttaaaaaact tgcttgagta
     1141 ccagtagtac ctttaactcc tcttaacttc atgttgtcta ttctatagtt taaatcttcc
     1201 aaatcaagaa ttaaatcttg agcccataaa caagctcttt ttcctactgt tgtaagttga
     1261 gcagcttgga agtgtgtaaa tccaagagct ggcatatctt tgtacttaag tgcaaattct
     1321 tttaaattgt ttattaaatt tacaagcttt actcttagaa gttttaaagc ttcattcatt
     1381 tgtataacat ctgtattatc tccaacatat gcagatgttg ctcctaaatg tattattcct
     1441 ttggcttttt cacattgtaa accataagct tttacgtgag acattacatc atgtcttact
     1501 tctttttcta tttttctagc ttcatcaaaa tttatattat ctatattagc tcttagctca
     1561 tctaattgtt cttcagttat attgattcct aactcatgtt caccttctgc aagtgcaacc
     1621 cataaccttc tccatgtaga gaatttaaat tgtggagaaa atatataact catgtccttg
     1681 ctacaatatc tgtctgtaag tgggtttgag taagtagtat atttattatc tatcattata
     1741 ttagcacctt ccttaaatct tttgtcgtgt gattactgtg tacacattag tacataaata
     1801 attatatata attttacgaa ctataacaag acttttttat tttttgttca catttatata
     1861 tagtattttt tatttattac tattttttat ttatatccaa attatctttt tcaattaagt
     1921 aatccactat ttcactatca acaaaattta atacatactt atatctaatt gcatagtatt
     1981 tatcactcca tccaagctga ttttttacat cactttcatc tgccccaagc cttaaactta
     2041 ttgctgcaaa tgaatgtcta aaatctcttg ctgaatattg agaaagtcca gctaaatcta
     2101 atgctttttt gactataagt ctgacatttc tatctgttat agaattactc ttttgagttg
     2161 taaaaataaa atcatcacta ggtattatta cctctggaag cccagaataa ttcctgtaat
     2221 cttctaaaag tttaaaaaag tatggatgta atttaactac tctttctttt cttcccttac
     2281 ctaaccttac atatgagtta tcgttttcat ctaccataag gtctttccac tttaagctaa
     2341 ctaattcatt taataaacac ccagtagtaa ctagacagac tattattact ctatctctta
     2401 tatttaattt tggtaggata cctataagtt tatttatttc ttgtatactt agaacatcat
     2461 cttttgtttt tattcttgtt actgtgggtt tttctacata tctaaatgga tttatatcta
     2521 tgtatccttc tttttttaag aagttataaa aactatgtaa ataactatat atcttttcac
     2581 aagttgattt cgcatattta gtctgtatat agtctacaaa atttttacaa tcttctttcg
     2641 tagcataaat caattctttc ccttttaaaa attctttaaa cagtataacc tttgataaat
     2701 aatcgtgttt cgtgtttgga tttaattttg tagaaaaaac attaaaagtt tcatactcat
     2761 aatcatctag tatttttgta ctatacataa taactccttt ccttttcata aaataaaata
     2821 gctatacttt tactatacca tatatttccc cctcaaatat accccttatt caatagataa
     2881 cttttaagtt tattttgtta tattgtgaag gttttatgaa aatatcaatc ttccattgtg
     2941 cactacacag tactatggta ttataagtta gtaaaaggtg gcattttttt attaatctat
     3001 gaataaaata tgctactata aatttgtatt ggaggtattt tgatttggaa acatttagtt
     3061 tgcttgaaat ttttaaggca gtcattttgg gcatagttca gggaataaca gaatggctac
     3121 cagtaagtag tacagggcat atgatattag ttgatgagtt tataaaattg aatttttcaa
     3181 atacatttat aagtacattt ttagttgtaa ttcaatttgg ttcaatatta gctgtacttg
     3241 ttatattttt tagaaaatta aatccttttg atagcgcaaa gaatattaag cagaaaaaag
     3301 aaacagtccg tttatggctt aaggttataa ttgcagttat accatctgga gttattggaa
     3361 tactgtttga agatgatata gatagattat tctttaattc aacagtagtt gctattgctc
     3421 ttattgtata tggtataata atgatagggc ttgaaaagag aaataaaaaa cctaagtata
     3481 aagatttttc tcaagttaca tataaattag ctctttgtat aggattattc cagtgtctag
     3541 ctcttatacc aggtacatct aggtctggtt ctactataat aggtgcagta ttacttggta
     3601 cttctagata tgtagcagca gagttttcat tcttcttagc catccctaca atgcttggag
     3661 ctagtgcatt aaaacttcta aaagctggat ttggatttac tggatttgaa tggcttattt
     3721 taggtgttgg ttctgtagta gcatttgttg tttcaattgt agttattaaa ttttttatgg
     3781 actatatcaa aaaacatgac ttcaaagtat ttggttatta tagaatagtc ttgggtatag
     3841 tggttcttgc ttatttcttt ctattataat gtattctaat ttaataacaa atgataagga
     3901 gaaaagttat gagtgttaaa aaagaaatat ttgattggat taagtcaata gctatggcta
     3961 ttgtacttgc atttgtaatt ctacaattta taagaccttc tattgtaagt ggagaatcaa
     4021 tgtatcctac tttagatgat aaagattatc tgattttaaa taggatatca tacaaggttg
     4081 gtaaacctga aaaaggcgat attgtagttt ttaaaaccaa tttagttgat ggagaaacag
     4141 gaaagaaaaa agacttaata aaaagagtta tagctactga aggtgacaga ataaaaatat
     4201 caaattctaa agtgtatgta aatggaaaat tattaaatga accatatata cacaataact
     4261 atacttctgg agatatagat actgttgttc caaaaggtaa actatttgca atgggagata
     4321 atagagaaaa tagtaatgat agtagattcc ctgatgtagg tatggttgat gaagatgaag
     4381 ttcttggtaa ggttatggtg agactattac ctcttgataa tattgggaaa gtagactaaa
     4441 aaataagaga ctgaattggt aaagtttatt ttacaaatct cagtctcttt ttattttctt
     4501 aaattacaaa tattttttta ctaaatatct tttgattctg taataagtct tattatatca
     4561 cttgcttctt cattaaaagt tgttactata cttaggtagt ctgttgctct actcatagct
     4621 aaataaacct tatttaaatc tcctacaaag tcatttactt gataatcttg ttcaatatca
     4681 tttatagttt gattgtataa catctctaat tcacatacta ttaccgcttt atactctaaa
     4741 ttctttattg catatatatt tgctattgta gttcctacct tcttacttat atttgtcaaa
     4801 ttatcttctg cacatatata aggaattttt gcctcttcca atgctttttt taacatatat
     4861 tgaaagtaaa tggtttttcc actttttaat ttcttcttat tgtaaggata aacgatagca
     4921 acttcagaat aatcaagacc ttttttactt attaaatact caatttccca gattacagaa
     4981 cttatttggt catcaaggtc acttactttt attatatcaa cagacttatt tccacctcta
     5041 agtgctttag tataaaagaa agtactaaat tctgtatttg gtcttaattc gttgatatat
     5101 gagttagaat tatttgaaaa tttatttgta aaatctacaa tctctttagc ttgtctataa
     5161 tttttactta agataatctt atcatcaaat tctataccat tatataattt tttaaatata
     5221 ttcaatctat tagagatatt caaggagtta caagaacaca cattaaatat gaattttgtt
     5281 ttatataaaa actcccttat aaaatctatt tcatactcta aaaagctttc tgcttcatca
     5341 ataaaaatag ccttgaacat atttttattt tttataatgt tctgtgcttg ttttacaaga
     5401 ttattaaagg tcttttcgta atcatttttt aacatgttat aatctacaat taaattgaat
     5461 tttttagcta gtttaaatat aaaagaacta aatgtgtgaa cttctaaatt gttattatct
     5521 ttgtataata actctatcct ctctcgaagc tcattgcaca attgttttgt atgtgtaaat
     5581 atgactaatt tatgatgtgg atatactttt gctaatttta tagccctaga aagcataatt
     5641 gctgttttgc ctataccaga accaccttca ataagtgtat tcccatattt aatagaaaca
     5701 acatctttaa tttgaacgtc ttccatcata gttgctgtat acttatagtc atcattgtag
     5761 aatgaaattt ttttaaattt ttcatttaga tgaagtttat catttattat atagtattct
     5821 gaacatatgt ctagtaaaaa taaatttaag tttatttcat cattctcatc attaagatat
     5881 tcctccagcg aacccttatt cattatatct tgaagcttat ttttatctat tatattatta
     5941 ttaacaaatt cttcaaattc atatgtttcc tcaacttcaa cgtatggcat aatgtaaacg
     6001 tagttataac ttatatttct attcttttgt cccattttta gtttcaaaag ttcatattcc
     6061 tcattcataa cttcaagtaa ctcttcttct aaaataaaaa atatatcttc tgttgtatcc
     6121 ataaatttta taaataaaat tcttccatct ttaacataca acaaatcggt atttattcct
     6181 ttaaaaggag taacttttgg cactacttta acgtcctcat tgatattttc gtaaaatatt
     6241 ttctcgaact ccttcaattt tagggcgtct aactgagaat ttatatttat gtattggctc
     6301 acttacatta cctccttctg ttatacttaa ttataccatt ttgaactata tttttatata
     6361 tactttttat aggcaaattt agtaaataaa ttcaaaataa atacaaataa atacaaaact
     6421 gctaaaaaaa ttttgcaata agtatagata tttattgtta taaaatactt tctgtaaata
     6481 ataaaatgat aaatttcata attaatgctt aaaataataa gtaaagtctt aaaaaaaata
     6541 aatcttgtta gc
//
LOCUS       DAEMGG010000172         6540 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEMGG010000172 DAEMGG010000000
VERSION     DAEMGG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.173"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            356..1300
                     /locus_tag="KPK24_003477"
     CDS             356..1300
                     /locus_tag="KPK24_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG2301111.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1322..1948
                     /locus_tag="KPK24_003478"
     CDS             1322..1948
                     /locus_tag="KPK24_003478"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG2301112.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1982..3031
                     /gene="hisC"
                     /locus_tag="KPK24_003479"
     CDS             1982..3031
                     /gene="hisC"
                     /locus_tag="KPK24_003479"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG2301113.1"
                     /translation="MREKESIRELKGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            3034..3612
                     /gene="hisB"
                     /locus_tag="KPK24_003480"
     CDS             3034..3612
                     /gene="hisB"
                     /locus_tag="KPK24_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG2301114.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3614..4219
                     /gene="hisH"
                     /locus_tag="KPK24_003481"
     CDS             3614..4219
                     /gene="hisH"
                     /locus_tag="KPK24_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG2301115.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4216..4938
                     /gene="hisA"
                     /locus_tag="KPK24_003482"
     CDS             4216..4938
                     /gene="hisA"
                     /locus_tag="KPK24_003482"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG2301116.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4920..5678
                     /gene="hisF"
                     /locus_tag="KPK24_003483"
     CDS             4920..5678
                     /gene="hisF"
                     /locus_tag="KPK24_003483"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG2301117.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5706..6374
                     /locus_tag="KPK24_003484"
     CDS             5706..6374
                     /locus_tag="KPK24_003484"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG2301118.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2676 a          549 c         1267 g         2048 t
ORIGIN      
        1 attttttaaa taactattgc caatttttga aaaatatatt attattaata gtaacaaaaa
       61 actgtaaatt tagaatatta ataaaaatta tttgggagga aattatcatg gtgagattaa
      121 atatagtaaa attaaataca aaattaaatc atcaccatca tcatagatta aagggattgt
      181 aatatgaaat taattcatag acacaagccc tgtttttagt gtaattggtc ttgtatctat
      241 gatgggaagt aattttaaga gaaaattata gccatatagg tataaagcct atgtggcttt
      301 tttattgttt taaatttgca aatataagag taaatttaat gaagagggtg agaatgtgaa
      361 atttttgagg attgaagatg aggttattta ttctaaaaaa agatataaat taaaaaggga
      421 aatagataaa ctttttttgg atgaagaata ttttcaaata gaaccacaat tatttgaaga
      481 atatgatgaa tttacaacta taaataataa aattccagaa gaatctatgg taaaggttgt
      541 aaatggaaaa gtaatggtac ttagagctga tataactaca agtataataa aaagccttgt
      601 accaagatgg gaagatggtt taaagcttaa gcttttttat aattcatcaa tatataaaaa
      661 taaaaataca gttggaataa aagagataag acaaataggt tgtgagtatc taggagaagc
      721 ctctgtagaa gcagatagag aagtagtgaa attagctctt aagatacttg aaaaatacaa
      781 taataatttt attctagagg ttgggagtag caagtatatt catggtttgt tagaagaatt
      841 aaatttaaat aagaattgtg aaaatcaaat taaaaattta ctatatacga agaacactca
      901 tgaactaaaa gtatatattg aagaattaaa aattaagcgt gaagtaaaag agttattatc
      961 aaatatttta agtttacaag ggaatttata taatgttatt gaaaaatcaa acaagttcta
     1021 ttgtaataat aagatgaagc aagctcttga agaattaaag caagtaaata acttgattga
     1081 ggaatgtaat tttttagata aggctagatt tgatttatca atgattacta tgcttgacta
     1141 ttatgaagga attatgttta gaggatacta cccaaactca tataaagaaa ttcttagtgg
     1201 tggaagatat gattctttaa ctaaagagta tggaaaagaa attccagcca ttggatttac
     1261 actaagtgtt gatgagttaa tgaagtatgt atataagtaa agtttagaag gagggattgc
     1321 tttggattat ttgactatag cactagcaaa aggacgaatt gaaggggaat catttaaaaa
     1381 atttaaaaaa atgggtctag gagacagtat agacacagat acgagaaaac taatttttaa
     1441 agatgaagaa aataaaatta tatatataca tgtaaagcca tcagatgtag ttacatatgt
     1501 tgaaaaagga gtcgctgacc tcggtatagc aggtaaagat actattttag agaatgagac
     1561 agatgtatat gaaatatatg atttaggttt tggaaaatgt aaatttgctg tagcaggatt
     1621 aaagggagat agcatatata gagaagatga atatttaaaa gtggcaacaa agtatcccaa
     1681 tatagcaaaa aagtatttta aagagaaagg tcaaaaaatt gaaataataa aattaaatgg
     1741 ttctgtagaa ctagctccaa tagtagggtt gtcagatgta attgtagata tagttgagac
     1801 tggcaataca cttaaagcaa atggtcttga aatattagaa gacatatgta atataagtgc
     1861 tagaattata tcaaatagag ctagctatag atttaaatat gaacaaattc agaatataat
     1921 tcgtttattt gaagaacttg ataattaaaa tataattgag ctttataagg agggatttaa
     1981 gttgagagaa aaagaaagca taagagagtt aaagggatat gaaccaaatc acgtaaactg
     2041 taaagtaaaa cttgatgcaa atgaaggaag taagaggttg tttaaatacc ttattaaaga
     2101 aatttcagat agtgatatag atttaaattt atatcctgaa gattcttata gtgatttgaa
     2161 agaatctata atagattaca taaatatttc tggagtaaat aagaaaaatc ttctagtagg
     2221 aaacggttca agtgaaataa ttgatttaat aatacatact tttgtagata aagatgaagt
     2281 aatattgagt ttctctccaa gttttagtat gtattctata tatagtcaga taaatgggtc
     2341 taaatttata ggtgttgaaa gtgatgaaaa tttagttata aatatagata atgtgattga
     2401 aaaagtaaag gagaataatc ctaaaatagt aattgtatgt aatcctaata atccaactgg
     2461 aacaatatta aagagggaag agataataaa gcttttagat tcaactaata gtctagtggt
     2521 tttagatgaa gcatatatgg attttggaga agaaagcatg ttaagtgatg tttttaagta
     2581 tgataatcta atagttctta gaactttatc aaaggcattt ggattggctg gaataagaac
     2641 aggatatatg ttatctaata gtagtttaat aaattctgtt gagaaagtaa gacctccata
     2701 taatttaaat tcattatcag actttattgc tacaagagca cttagaaata aagatgtggt
     2761 aaaggcttat ataaaggaag taaaagagga aagagaagtt ttatataaag aaatgattgg
     2821 tatgggaatt aaagcatata aatcacaagc aaattttata ttattttatt ctgaaatcga
     2881 aaatttatca caaaaactta ttgatagagg tgttttaata agaaaatttg gtggaaagtt
     2941 agaaaattat tacagagtta caattggtga taaagaagaa aattctatgt ttgttggagc
     3001 tataagagat attttgaaaa aggagaaata ggtatgagaa tttggaaggt agaaagaaat
     3061 actcttgaaa cacagatttt agtagaatta aatattgatg gttctggtaa agctgaaata
     3121 gatactggaa taggattttt agaccatatg cttaccttaa tgtcatttca tggcaagttc
     3181 gatttaaaag tgatttgtaa gggtgataca tatgtagatg accaccatag tgtagaagat
     3241 ataggtatag ctataggtga agcatttaaa aatgctctag gtgacaaaaa aggaatacga
     3301 agatattcta atatctatat acctatggat gaatctctat caatggtagc aatagatata
     3361 agcaatagac cttatcttgt atttaatgct aaatttgata cccaaatgat tggtagtatg
     3421 agtactcaat gctttaaaga attttttaga gcttttgtaa atgaatcgag agtaacatta
     3481 catatcaatc tcttatatgg ggaaaatgac catcataaga tagaaagtat ttttaaagca
     3541 tttgcaagag ctttaaaaga gggtagtgaa atagtttcta atgagatagc atcttcaaag
     3601 ggggttttgt agaatgaata taatagtaga ctatggtctt ggaaatatag attctgtgtc
     3661 taggggtttt agaaaagctg gaatagaaac taaaatttct agtgatatag atgaaataaa
     3721 acaggcagat tcacttatac ttccaggtgt tggagctttt agagattcta taagtgcctt
     3781 agataaacta ggattgatac caataataaa ggagcatgtt tctaagggta aatttatgat
     3841 aggtatatgt ctaggaatgc aattacttta tgaaaaaagt tatgagtatg gagaatatga
     3901 aggattagga cttataaaag gaagtattga taaactggat ataagtttaa aagtgccaca
     3961 tatgggatgg aataatctaa aatttaataa ggcaaatgat gatatactta aatatataaa
     4021 tgaagatgat tatgtatact tcgttcactc atattatgca aattcatcaa atgaagaact
     4081 aattgcattt tcagaatatg aaaagaaaat ccctgctatt gttagaaaag gcaatgtata
     4141 tggtatacaa tttcatcctg aaaaaagtgg agaagtaggg ttaaatatat taagagcata
     4201 tggggagatg ataaaatgat aatatttcca gcaatagata taaaagataa taagtgtgta
     4261 agacttacac aaggagaatt tgacaaagta aatgtatatt atgataatcc attagaagta
     4321 gcatataaat ggaaaaatga aggagcagaa tatatacata tagtagattt aaatggtgct
     4381 agaagtgaat ttggtgtaaa tacaaaaata attgaggaca tagcaaacaa tatagatata
     4441 cctattcaag ttggcggtgg agttagggat aaggaaaaag tcaaaagttt aataaatgct
     4501 ggtgttacta gagtaatatt aggtagtata gcgattgaaa atttgaattt agttgaagaa
     4561 ttggtcaatg aatataaaga gaaaattgta gtttctatag atgctaaaga tggaaaagta
     4621 gcagttaggg gatgggaagt tgtaagcaat gtagactcat taacattatg caaacaactt
     4681 gaaaagatag gtgtacaaac tattgtttat acagatatat caaaagatgg gatgttgcaa
     4741 ggtccaaatt ttgacatata tgaaagaata gcaaaggaaa catcactaaa tgttattgct
     4801 tctggaggcg taacttctat agaagacgta aaaagactaa aagctatgaa cttatatggt
     4861 gcaataatag gtaaagcgct ttatgataaa aagatagact ttaaggaggc acaacaatta
     4921 tgcttactag gagaataata ccttgtctag atgtgagaaa tggaagagtc gtaaagggaa
     4981 aaaaatttaa agatatagta gatgttgata gtccagaagt acttggaaaa ttttatagtg
     5041 attgtggtgc agatgaactt gtattctatg atataactgc atcaaatgaa gagagaaaga
     5101 catctttaga atttgtgaca aaagttgcag aaaatataaa tataccattt tgtgttggtg
     5161 gaggagtaaa taaattagaa gatttcactg atattttaag aaaaggtgct gataaagttt
     5221 ctataaattc atcagcagtc aaaaatcctg aattaataag ggaagcttca ttaaaatttg
     5281 gagcacaatg tgttgtttta tcgatagatg caaagaaaaa tgaagagggt tcatggagcg
     5341 tatacgtaaa aggtggaaga gaaaaaacta atctggatgc aattgaatgg gctgttaaag
     5401 gtgtagaact tggagcagga gaaatcgttg taaatagtat ggatgaagat ggaatgaaaa
     5461 atggatatga tatagaatta ttatcaaaga taacttcttt agtaaatgta cctgtcatag
     5521 catctggagg cgctggaaaa aaggaagatt tttatgaggc ggttaataaa tccaatgtag
     5581 atggaatttt agcagcatct gtttttcatt ttggcgagat aaaaataaac gatttaaaga
     5641 aatatttaaa agacatgggt gtagaagtaa gattataagt taaatactaa aaggagatta
     5701 gaaatatgga taataagtgt aacaatgtat acagtgatga agtagagaaa tttataagaa
     5761 gtataaagtt tgatgataaa gggttagtcc ctgttgttgt tcaagaggta gttagcaaag
     5821 atgtactgat gttagcatat atgaataaag atgctataaa gaaaacctta aaagataaag
     5881 tagcttgcta ttttagtaga agtagacaag aactttgggt taaaggtgag acatctggta
     5941 atactcaaaa agtagttaag atgtcttatg attgtgatgt agatactata ctactttttg
     6001 ttgaacaaac tggtgtagct tgtcatacag gcaattattc ttgtttttac agagacttgt
     6061 ttgatgatag ttcaaaaatg gagcttgaag tacaaacaaa tatattgaaa gaattatatg
     6121 atttaatcaa tgaaagaaaa aataatccag ttgaaggttc ttatactaat tatctttttg
     6181 aaaaaggaat agataagatt cttaaaaaag taggtgaaga gagtagtgag gtaattatag
     6241 catcaaaaaa taccgataaa agtgaactta tttacgaaat tagcgattta gtatatcata
     6301 cattggtttt aatgatagaa aaaggtgttg aaattgatga aataaaaaaa gaattactta
     6361 aaagaagaaa atagaataag atagattaat gggtaataag aaaatatacc acttttaata
     6421 aaaagtggta tattttttat tttatatttt gatttataga ataatattta aattacttga
     6481 taaaattaac tatatatata atttattgat aaaataattc tagtacatta tttattttag
//
LOCUS       DAEMGG010000173         6524 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEMGG010000173 DAEMGG010000000
VERSION     DAEMGG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.174"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(342..749)
                     /locus_tag="KPK24_003485"
     CDS             complement(342..749)
                     /locus_tag="KPK24_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301119.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1223..2107)
                     /locus_tag="KPK24_003486"
     CDS             complement(1223..2107)
                     /locus_tag="KPK24_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2301120.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2398..2874)
                     /locus_tag="KPK24_003487"
     CDS             complement(2398..2874)
                     /locus_tag="KPK24_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG2301121.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3140..4315
                     /locus_tag="KPK24_003488"
     CDS             3140..4315
                     /locus_tag="KPK24_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG2301122.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4396..5199)
                     /gene="proC"
                     /locus_tag="KPK24_003489"
     CDS             complement(4396..5199)
                     /gene="proC"
                     /locus_tag="KPK24_003489"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG2301123.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5239..5823)
                     /locus_tag="KPK24_003490"
     CDS             complement(5239..5823)
                     /locus_tag="KPK24_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301124.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2168 a         1095 c          720 g         2541 t
ORIGIN      
        1 attatccgtt aaggtctacc cacttccact accaatagct tgtagtgtac ttccctcaag
       61 aggaatagtc gttgaagttt ctcctgttcg gagtttacct gctgattgcc cattttaaag
      121 tacttaggat ttaaccttat accatctaac caattttttc tactttcgta actttcacac
      181 ttaggtttat ttcatcctta tgttttagtt tgattagctt taggggtttc cagcaattca
      241 agtagtattg gataggttct atcctatctc tacatatagg tttccctata tgctgactat
      301 tttagctact aactcatgac taaagtcacg agtgtgtgta gctatttttt aatcaatatt
      361 ttagtaaatt ttacggtaag taaaaacctc tctccttcaa atcctatagc aaatatactt
      421 acaagttgct ttgagaaaat aactcctaat atagctacaa ctatgcatat accaactatt
      481 atatttaaaa cattgtttaa aaactttagt gcttgctttt caccttgttt actacttata
      541 tcttgataca ttggaataaa agtagtaact atagcagtac ctattgctgc aaatataata
      601 tttggtatat tcatagctgt taaataactc tctgtatata agcctgtacc ataaattgat
      661 gataatacta attctctacc cattccaaga atcttagaga ttatagtaac tatcatcaaa
      721 taaaaagttg ctttagctac cttgctcatt ttatcactcc acttctaaca tacctctttt
      781 taatcaagta aaatttagct cattagatat aattctatca actaatttgc cttgcttagc
      841 cctatcaaaa tataattaac acctatttct taatatatca caaactacat tattcttcat
      901 atttttttat ttctccaata agttttacat ttttcctata taaattgtat agcttcaatt
      961 atattcaaag aaatggagtt gctacccgat aactttaaaa gtaaaaaaca gattttatta
     1021 tctttttaaa ctatattcta acatattttg tccatattga gaaattttac taaactatta
     1081 tgtactttat taaataaata aaataacagc acacctataa aatacaagtg tgctgttcat
     1141 aaacagattt tataaaaaaa taattcctaa gtagagatta acatgctttc tcatactctc
     1201 atatataatg tgtctaaaaa tattaataaa aatgttttaa ttgttttaac ttacttggat
     1261 gtcttaattt tcttattgct ttagcttcta tctgtcttat tcgttctctg gtaactccaa
     1321 atacctttcc aatctgttct agtgttttag ggtcatcatc atcaattcca aatctcattc
     1381 ttaaaacttg ctgttcttgc tctccaagac ctgtaagtag ttcttctatc ttttctttta
     1441 aattatgctg ttcaacttcg tgtattacaa catctttaaa atcagcatct gatggtataa
     1501 attcaactaa actactatcc tcatcttctt ttaatggagt atccaaagat acaacatttt
     1561 tatctcttct taataattcc aacactttgt caacttctaa gccacatgca tctgcaatct
     1621 catctatagt tggctctctt aataatacat ttaaaagttc ttgtttcttt ttctttacaa
     1681 aatttattct ctgatgaaga tgtataggta tcctaatagt attttcgcaa tcgtcgatat
     1741 atcttgttat actttgtttt atccaccaag ttgcataagt gctaaatttg tatccttttt
     1801 tataatcgta cttttctaca gcttttataa gtcctatatt tcctgcttga attaaatcca
     1861 acatatcaat actatctctt ttatatctct ttgctatgct cactaccaac ctataatttg
     1921 aatttataaa tttttctttt gctactgaag atgagttgat tatttcctta gccagctcta
     1981 cctcttctcc tgctgacaac attttgtatt cctctatttc ctttaaatac attttcatcg
     2041 cattcgatac atttgatgaa cccacacaaa tataattttc ataattattt tcttttttag
     2101 tatccataat caaacccctt ctattcatag aatttgctaa aacaaaaata aataatttaa
     2161 acctacataa tctacaatta cataatatcc cattctttaa atgaattgtt ttgtatatta
     2221 tgtatacaaa tttataaaaa ctatacctat aatattattc tacttctaac agcaatatcc
     2281 tccttatata tataatttat tttattatat gttgttatat tcttagtttt ttgaatgata
     2341 tatgtctaaa aaaagactgt ttactaaaca gtcttttttt atatatatgc tcaatattta
     2401 attttcatga tgatgattac atccacatcc acaattttca tcatggtgat gatgttcttc
     2461 taaatctttt acctgttgtt ccatttcatg aaactctttt accattatat cataagttgg
     2521 aagagctgcc tcagtatcgt attcttcccc tctccaatca gcgtacatca tagcatctcc
     2581 attagttaaa accaatctac ctgacatagt tatattatcc acttcttcta aaaattctat
     2641 ttgtttttgt aaaacttctc cttctaactc cacatcttca tctaaacatt gcactatcat
     2701 tacaggtgca taataatctt cttcatcttt aacatttaca acaatatcta aaattccttc
     2761 tgcaccttct tcaaattctg ccaactcaac atttgtatca atcatatctt gaggtactaa
     2821 taaatcttct attaaatatt ttattactct atcttttact aattcttttg acatttattt
     2881 ttctccttcc gattctataa cattatcata atcatttcta tattatagta tactacatta
     2941 attgtttttt caatctctta caaaaattat tttttattat cttccttata ttcccttttt
     3001 tattacaaag ttgtaatcgc attgatttaa aaaatagttt atatttagta taatagaaat
     3061 gtagcacata tttttattca atatattaat aaaaaaatat attgaaaatg taatgtatgt
     3121 taaatctagg aggaatatta tgaagtttaa aaaacaaatt agttctattg ttgtgtctac
     3181 tatgcttgta cttgggagta tgaacctttc ttatgcagac ggtacaaatg tagttacact
     3241 tggagctaac ttatcagagt ctcaaaagca gcaaatgctt aattactttg gagtaaaaaa
     3301 agaccaagtc ttagttttag atgtaacaaa tgcagaagaa agacagtatt tacaaggtgt
     3361 tgcaacagag ggtcaacttg gaaaagttac tatctcttgc tcatacgttg aacctactaa
     3421 aaaaggaaat ggaattaatg ttaaaacagc aaacttaact tgggtaacta gttcaatgat
     3481 tgcttcaacc cttacaacag ctggtcttga agatgcaaat gttattgctg ctgctccata
     3541 tcctgtaagt ggtactggag cattgacagg aatcatgaaa gcatttgagg atgcttcagg
     3601 aaagaaactt gatgaaacta agaaagaaat tgcttctgaa gaattggtag ttacaggtga
     3661 cttaggagat gatatagggc aagaaaaagc aacaggtgta atgaatgata taaaaacaga
     3721 aattgtaaaa aatggtacaa aagataccaa tcaaatagca aatacaataa ataatgttgt
     3781 aaataattac aatattacta ttacaccaga acaaaaagaa caaattaaag gtgtaatgaa
     3841 aaaaatagct gaccaaaatt acgattacaa tagcatgaaa aatacactag ataatgtatc
     3901 agataatgta aatgaacaat taaagaaatt aggtgaaagt gttaagggtt ctggtatact
     3961 agatactatt ggtggatggt ttagtggaat aggagactgg ttctctggtt tattctcagg
     4021 cggagataaa aaagatttgg gaatcttaaa taatactaat gatgagtctc ttggctctga
     4081 tgctgtaata aattctacag aaggtgttca aataaatcca caaaataata ctaattctaa
     4141 caatgatagt gatacaaata gctcaaaaaa tgatacttca agtaataatg aaaataacga
     4201 tagttctcaa acaaatgaaa acaacacaac aaataattca gaaaatcaat caaataataa
     4261 tgactcagtt ggtttctttg aaaaaataaa aaattggctt tcaggcttat tttagaaatt
     4321 aaacaaataa aaagaattgc ctaaaatgga aattttctat aaaaggcaat tctctttatt
     4381 tacaataaat ttttattact cactcatttt tttagatttt tctatacatg cacatattgc
     4441 ttttatcaca gaagctctaa aaccttcttc ttcaagtact ttaactgctt ctatagtagt
     4501 tccaccaggt gaacatacca tatcttttaa ttcaccagga tgttttcctg tctcaagtac
     4561 catttttgca gaacccataa cagcctgtga tgcaaactta tatgcttgtg gtcttggcat
     4621 tcctgcaatt actgctgcat ctgctatagc ttctataaat aaaaatacat atgctggtgc
     4681 agaaccactt gcaccaatta cagcttctat aagatactct ggtactacct cagtatttcc
     4741 aaaagaatta aatacttctg taactgcttc taagtcttct ttgtttatat ttttatttat
     4801 agaaagagaa gacatggctt catttacaag agctggagta tttggcatag ttctaactat
     4861 ttttttatct tctcctataa tagactctat atcttttata gattttcctg ctgctattgt
     4921 aactataatt ttattgtcat ctataaagtc ttttatctct tccaaaatat catcatatat
     4981 atctggttta acagctacaa ttactatatc agaggttcta gttacttcct tcgagtcttt
     5041 tgtagtattt attccaaaat ttgcatctat tctatcaaga gttccttgac tatatgctga
     5101 tgctataacc atattaggtt caactaattt tgaatttact attcccccta tcatagcgct
     5161 tgccatattt cctgcaccta tgaatccaat ttttttcact ttagtacctc cttattgact
     5221 aatagtaatc gttaaaattt aagcttcttt aactgcatca gacttttcaa atggatttgg
     5281 tatacttact accaaaattt tcactacctt attagtatag ttgacccaat tatgattgtt
     5341 cttttcacta ttatagtgta ttgtgtctcc tggatacatt tctatttgtt catctcccaa
     5401 aaacactgta agagtaccct ctaatacata cacaaattcc tcaccttcat gtacgtaaca
     5461 acaaatttcc tcactactct tacttggaag aatttcaatt aatcttggta gcattgtttt
     5521 ttcctttaaa ctagatgata aatgataatg tataaatgtt gagttttcta catcaaatac
     5581 ttctttttca taacttctta atacagctct tttatgctct tttggcttca taaaaaagta
     5641 agttaattct acatctagta cacttgctat ttttcctaag gaatctgttg ccacacttgt
     5701 taatcctctt tcaagttgtg ataaaaaacc aattgacaag tttgtttttt cactcatatc
     5761 cttcagcgtc atttgctttt gagttctgag ttgctttatt tttgctccta tgtccttatt
     5821 caaaaccatc acccctaaat aattttaatt ttttcataat ataaatacaa aaatataaat
     5881 ataggtactg aacctatctt tatttcattt aaaaaaattt ttataagtat tactataatt
     5941 tatcaatctt tttttgtcta catttttacg attatttacg atttattttt cacatatgta
     6001 tatttaatct tgccaactaa agatacattt tttataagca tttactcttt atataataaa
     6061 acttaagttg ttcatgagtc ttcttctggt ttaattatta aaatttcaaa ttatattatt
     6121 atttctacac attattacat cctttagtac aattataaca taattttaca atttaatgat
     6181 atctattcaa tgtctttttt tactctacaa catattttat tttagagttt aatactatat
     6241 atatttttta tatacagcct tgtcattttt tagattgtca agagttattg aagtatttta
     6301 tgtagattta gattttattt tctaatttct catttaacaa gaattgctta tgataaaaaa
     6361 taatatcatc tcaataagat tcttgtaaat atatctatat tctaaatata atttcttttt
     6421 ttgtcaaaat ttcttcattc tttaataatt aatttattac ttataaaata aactaattat
     6481 ctatacaaat tttataattt ttaataaaat acacaaaaaa atta
//
LOCUS       DAEMGG010000174         6516 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEMGG010000174 DAEMGG010000000
VERSION     DAEMGG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.175"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(98..349)
                     /locus_tag="KPK24_003491"
     CDS             complement(98..349)
                     /locus_tag="KPK24_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301125.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            complement(380..1399)
                     /locus_tag="KPK24_003492"
     CDS             complement(380..1399)
                     /locus_tag="KPK24_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG2301126.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            complement(1417..2370)
                     /locus_tag="KPK24_003493"
     CDS             complement(1417..2370)
                     /locus_tag="KPK24_003493"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG2301127.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            complement(2712..3488)
                     /locus_tag="KPK24_003494"
     CDS             complement(2712..3488)
                     /locus_tag="KPK24_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2301128.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            complement(3522..4043)
                     /locus_tag="KPK24_003495"
     CDS             complement(3522..4043)
                     /locus_tag="KPK24_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2301129.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            4639..4905
                     /gene="rpsT"
                     /locus_tag="KPK24_003496"
     CDS             4639..4905
                     /gene="rpsT"
                     /locus_tag="KPK24_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG2301130.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(5092..6135)
                     /gene="holA"
                     /locus_tag="KPK24_003497"
     CDS             complement(5092..6135)
                     /gene="holA"
                     /locus_tag="KPK24_003497"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG2301131.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(6089..>6516)
                     /locus_tag="KPK24_003498"
     CDS             complement(6089..>6516)
                     /locus_tag="KPK24_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG2301132.1"
                     /translation="IIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPFVG
                     VIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKFYI
                     VVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT         2152 a         1067 c          702 g         2595 t
ORIGIN      
        1 tcctataaaa tacagttaat gaatttttat tttatacatt aaaaatatat aactaaaata
       61 aggcttattt taatcaataa accttattca cttattacta cttattaaat ttttttatga
      121 tttcatgtat atcaaaattt ggtatgtcaa aatcaaattt tgttatatca aatttaatac
      181 ttggaaaatc atattgtttc attcctagca tactttttgc attactgtcc actataaata
      241 cacaaactga tgtacatata atcatagtaa tcaaaaataa gattctcata atatacttgt
      301 attttcttag tttactttta ctttctatat ttttttctaa tcggctcatt tagcatgctc
      361 ctaattataa cttaaagctc taattttttt gtttaaaata ttcatccaaa atctcggcta
      421 aatactttgt agtagcctta gattcttcta cacttgttgc gttattccct atttctacta
      481 acatatgatt cttaactgca aactgattga atctagcata gtctttttct actattggag
      541 atactagacc aggatacttt ttctcagcaa aagcagttat atcttctgct aaagctctaa
      601 gttgttttct atttgtattt ttacctccta caacaaagaa gaactttgat actctctctc
      661 catttatcat agttgtatat tttaaatggt ctttttcctt agtggctgga ttagttaaat
      721 ccctagcatc tctatgtaag tctatcgcta tatccactga gttatattta ggtaatatac
      781 tttgtattgt ttttaagcta cttgcataag agttattata agatggatag tcatggtatt
      841 tagtagtatg gactacaccc catcctttgc tatctaaagc actagttaat gcacttccta
      901 cttccattac tgaatttttc ttatctcttg aatgataatt tccatcaggt gaatttgaat
      961 aagtctcaca tccatgagta tgatatatca aaattcttgg attttctttg gttactctta
     1021 aatcatcctt ataatctgaa ctagatgttg tgttagtttc agttgtgtct gaattttttg
     1081 actcaatatc tggaacattt tctttaccaa cataaagttt tatatattct ttttcactct
     1141 cttttttatt atcaacttta cttgcatctt tagatttagt gtttccttct ttactttctt
     1201 ccttattttc cttacttgtt tctttatttt tattattctt tttattttct gtatcatttc
     1261 cttcgacttt agcttctgga taagatgaat ttactaaaaa ctttaaaaaa tcatcttgat
     1321 tcaaagctaa tgattttcct ggtaaaatgc atactaatat aaaagtcaag gttactactt
     1381 taatgcattt tttaaacata attgctcctc cttattttat accaatctac ctggatgtaa
     1441 agacctattt aagccttcac ttattattat agatagattt tcgattgtat catctatatc
     1501 ctttggtgtt actattaagt tcttgtcata tgggtctaac gaatctttta ttagatgata
     1561 tttttcttct tctttaagtt ttttcaacat ttcataaaaa ctttctgttt cttctgattg
     1621 agctattaaa ttgtctattg ccatatctaa gacatctatt gtaagagttg ctgcatcaac
     1681 caccgttgga acgcctattg caataacatc cacgcctaaa gtttctttag ttagagattt
     1741 tctagtattg ccaacacctc caccaggtga tattcctgct gttgatatct gtatagtaga
     1801 gtttactctc tccatttttc ttgaagccaa tgcatctatt gctactactc tatctggctt
     1861 aaccttctca accaatgatt ttactatttc actagtttct attccagtaa cacccataac
     1921 tcctgggctt aaagctgata cctcagtgaa atcatcatca tagtctttat tataattttt
     1981 aaaaagatgt ctagttacaa gtgtttttga tacactcttt ggtcctaacg catcagaagt
     2041 aatatttcta tttccaagac caattaccag ggttttttta tttttgtctt ggccaaatat
     2101 atctactaac tcctctttta aataacttat catttcttct cttgactcat catcatcaaa
     2161 cttcataaga ttactttcca gagttatata ctttcctata tctttattca ttatctctgc
     2221 gccttgcctg tctattactt caactttagt tacaatgcaa ttttctaatt ctttagtttc
     2281 tatttttaca ccaggaatac tacttccttc ttgagacttt tcacacattt cactagcttc
     2341 taaagctaaa tctgttctta cactaatcat ataaacctcc agtataaatt aaatgctaat
     2401 tataattgtt aacaaaattt agtattttat aacaagaaat tgtaaattta tttaaaacaa
     2461 gtaaaattaa ggctttcaat gtaagaaaat tgggtttagt cagtgatttg tcagtaaacc
     2521 ttcttatcta tcatttattt acaaacagaa aaaggtagca actatttata taggtgctac
     2581 ctttaatcta tttataattt tcctattaag ctttcactaa tatattaact aaaataattt
     2641 tttatattta tacctaattt atttactata taaaaaaaca gcaataatta tacaataatc
     2701 acctaacacc tttattcctt ttggaaaaat ggttctgcaa tttccaacca ttcatttgga
     2761 agataaagtg ataaataccc atgattatag ctcttagctt cataaatctc acaagagggc
     2821 acataagact gaaacagttt tgcagatttt ttgacacact tcatttcttt ttctccatac
     2881 cagtacatta cttgtgctgt ggttttttta acattctctt taagtgtata gttcatcatg
     2941 taagtacgat acatattata gaatgtttct tttcttacat gtggcatatc tgtcatataa
     3001 tacgctttta tttcatctgg atattgcata ttttcaggta acaattttgg catcatagca
     3061 atttgaaagc gacaagcctt ctcactaaac aaaagtcctc caaaaaatcg aataccagca
     3121 atacaaaacc ttgccataag aggtcttggg tagcaaatac taccatcaat aattgctttt
     3181 ttagtaatat taggttttcg agataaaagt tccatgacaa tttgaccacc aagtgatacc
     3241 ccacacaaag cgaaaagatg tccactgcaa ttttcatcta tatatgatat caacttatct
     3301 gctgtatcct ctgtagaaat gtatgttgtt tcatattctt ctccatgacc atccaatgta
     3361 ggaagtatca catgatattt gttagacaaa acttctgctt gtcgcaaata gttccaccat
     3421 gcattccctc ccccatgaat caacataata tgtggtctgt ttttatttcc aaactcatga
     3481 aatttcatat ataacctcct tttataaaaa tgggttgcat gttattttgt tttatcatta
     3541 tcagctacag gtggagaaga tataattgaa agttttctaa gttttagatt tgatttaggt
     3601 ttttgttcta tatatggttt caataaatca gctatacttt taagaagatt ttcatattca
     3661 ttatcactca tatatagtaa tgctgaagag aaaaatacat tatccttttt atggtcttgc
     3721 tcttttctgc taaagtattc attcatttcc tgcatcagac ctacataaaa tgctgtagaa
     3781 agtgcaacga aggtctctcc ttccatagat gttgtatatt cctttagtgc ataaaccttt
     3841 tcaatagtac cacgcacacg attttcttta attacttcaa tcatattgtt ctcttctaaa
     3901 atttttacat ggtgataaac tgttgcacga ggaatatcat ttaaatactt tacaatatca
     3961 gatgtacgaa cactgccata aagtcgaata tattgtaaaa tacgaagcct tgcaggattt
     4021 aaaattagtt ttgctatgtc catcttatca gccctttctt tagtctataa aaatattata
     4081 gtctattttt ttagactatt caagtctatg atataaatag taattacaac tataataaat
     4141 gctacaaatg ctattctata taaacactga cattaattac atataacctt ctaacatatt
     4201 agttatattg tttgatgcat ctatcataca tactaatgaa aaaaattatc ttgattattg
     4261 gaaaaattat cttaattaag catataaatt tatattttta taatttttca ttaaatttac
     4321 aaaaatgagt aatttcttgt attatcttat aattattgga aatactatca aaatgtatgt
     4381 taatagtttg tattatctca aatatttgtt acaaaatgaa atattaatta tatatgtcaa
     4441 aatatttaaa acacaaaata taccaattta aaaacaagat attgcaaatt atttatacac
     4501 aatttacagt ctaaaaaatc aaaataaaaa acattattaa aatatataaa taataatcct
     4561 tgatttttag atttatttct gttaaaatct aatatgttga cgatgaatat gataaaaaaa
     4621 tacagggagg tgaacacggt ggcaaatata aaatcagcta agaaaagaat aagtgttatc
     4681 gaaaagaaaa ctgctttaaa tagagttaga aaatctcaaa taaaaactgc aataagaaga
     4741 ttcgaagatg ctgttgcagc tggaaacaga gaggatgctg ttgctaagtt ccaatacgct
     4801 caaaaaagaa tataccaagt agcttctaaa ggaactatac ataagaatgc tgcagctaga
     4861 aaagtagcta aattagctca aaaattaaac ggtatgaacg cttaattacc agtttatatt
     4921 tcttatatat aataacccct atgtaggggt tttttatttt gtaaatttat acaataaaaa
     4981 ataaatagtt agacatatta tagttgattg tatatcaaac ttaatttagc ttacatctac
     5041 tctgaactat aacatatctt gctatttatt ttttattaat atatagattt tttaaattgc
     5101 ttctctttta cagtatctac taaccaacat ttcaactgct aaagtatctc ttataagacc
     5161 tgtctttatt ttgtaatcac tttctaatat atagtttaat atttccacaa ttatatcatc
     5221 agaaaaattt ttagtttgct ttaatgcttt tcctacaaca aattgatgca tttttaattt
     5281 atcagctata agttttgttg aatatccatc aagttgtaac tgtcttactt gcataattat
     5341 tttaaattgt cttgctatca tagaaaatat acctaataca gattcgcctt cttgtatcat
     5401 atcatttaat attttcattg cattagaagc attttgttct ccaatataat cgattaattt
     5461 gaatatatca ttctcaactt ttttttgaga taatttatca attacatcat tggttacatt
     5521 attcccttta ccaacaaatg aacttatttt atttatttca ttttctaaat cagataatgt
     5581 cttttcacta ctcttatctc tgtatccttc ttgttcaata aaatacatga tttgagaatt
     5641 atctattata acatcattta aagcaaaaga tttttttatc catttaaaca aatccatatc
     5701 agaaagctta tcacaatcaa aaacaattcc attgtttcct attttcttaa ctaatgattt
     5761 tcttttgtct acatctccat atacaacaaa aactatagtt gtggtgtctg gaatattgtc
     5821 taaatgctct attaagtatt tctcatcact atctgtaaaa ttctttttct ttccttttaa
     5881 tagttcaaag tcttttatta ttactatctt cctatcatcc ataaatggaa gtgtttctat
     5941 agagcttata agctgattta agactatttc cttcccatct attatatcta aattaaaatc
     6001 aagcattccc tcgtttagac tatctttaaa tgcttttatt gcattctcaa ttaaataata
     6061 ctctcttcca taaaaaagat acattttttc aaagtttttc tcttttacat tccttataat
     6121 atccttgtaa ttcattctct tgctccttta tctcttttcc ttctatgtaa aatatgtaaa
     6181 aaaacactcc tatgtaataa ataacaacaa tataaaattt aggattttct atattaatgt
     6241 atgcaaaact taaattgctg attttttcta gtaaaaaaaa tatgctttct ataaatgtac
     6301 tattaaaaaa agatataatt ttagctatta ctacacttac tttaaatacg attaaacttg
     6361 caatacttaa atacattatt actcccacaa atggcactat aattaaattt cctataattg
     6421 aaagcaatgg tataccttta aatgtataat aaattatagg taaagttaat atatttgatg
     6481 atattgtcaa tgataccata cttaatttta taatac
//
LOCUS       DAEMGG010000175         6358 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEMGG010000175 DAEMGG010000000
VERSION     DAEMGG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.176"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            255..800
                     /locus_tag="KPK24_003499"
     CDS             255..800
                     /locus_tag="KPK24_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG2301133.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            813..1826
                     /locus_tag="KPK24_003500"
     CDS             813..1826
                     /locus_tag="KPK24_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG2301134.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            2048..4021
                     /locus_tag="KPK24_003501"
     CDS             2048..4021
                     /locus_tag="KPK24_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG2301135.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            4090..4449
                     /locus_tag="KPK24_003502"
     CDS             4090..4449
                     /locus_tag="KPK24_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301136.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4484..4840
                     /locus_tag="KPK24_003503"
     CDS             4484..4840
                     /locus_tag="KPK24_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG2301137.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4877..6169
                     /locus_tag="KPK24_003504"
     CDS             4877..6169
                     /locus_tag="KPK24_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG2301138.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2520 a          589 c         1194 g         2055 t
ORIGIN      
        1 aatttctaag aagaatttag aatatagtcg aaataagaat tgtatatttc tgaaaattct
       61 aattagtaca attttttcct ttataaaatt gaaaaaagat gtatttattt atcaaaaata
      121 ttatattaaa atgatattaa taaaaaaaca tttacaatat tagcatatag tacagaaaaa
      181 agatgtaatt tgtaaaattt atggtaagca gtaaaattaa aaatttaaag atattatagg
      241 gaggttataa ttttatggaa aacgttatta caggtctaag taaaggtgca gagtggttta
      301 tcggtttgtt tcagaaaggg ggagaacaat ttataggtct tgtttcaggt actcttccaa
      361 cgctaattgt attaatggta gctattaact ctttgattaa gataattggt gaggaaagag
      421 ttaataattg ggcttctaag ctaggaaaga actcttttac aagatatatt ctacttccat
      481 tagtatcagt atttttcttg ggaaatccta tgtgctatac atttggtaga tttttaaaag
      541 aagagcacaa agcaggattt tatgatgcag cagtttcatt tgtacatcca attacaggtc
      601 tttttccaca tgcaaatgca ggagaacttt ttgtatggtt gggaatatct gcaggtttga
      661 ctactttagg taaagaaact actacattgg cattgtggta ttttatagtt ggtctaattg
      721 taatttttat aagaggtata gtaacagaaa aaatgtatgc ttttctaaca agaaaaaata
      781 gagcaacaaa taaggcttag gaggtagata aaatggagga gaataaaata ttaaaaatag
      841 aaaagggaac ttcaggctgg ggaggtcctt tgtatataaa aaaagaaggg aatagaaata
      901 agatactgtc aatgactgct ggtggtatcc atgaagtaac attaaaaata aaagaactac
      961 tgggttgtga aattgtagat ggatttaaaa ctggagtttc tgatgaagaa gtagcagtag
     1021 ttattattga ttgtggtgga actgctagat gtggagttta tccaaagaaa aaaataccta
     1081 caataaatgt aaatccagtt ggaaaaacag gtcctttagc aaaatttata acagaagaat
     1141 attatgtatc agatgttaat cctaactgta taagtgttgt agatggggaa gatatgccac
     1201 aaaaatctca agaaaataaa agcgaaaata agagttctat tagaaagcct gataattatg
     1261 atgaggttaa gtcaaaagca caaggtgaat atgctaaaaa gaacataata ctttctattg
     1321 gtcaaggggc agggcaagta gtatcaaaat tttatgatgc aggtcgagat actatccaaa
     1381 tggtaatgaa taacgtaata ccatttatgg catttgtaag tatgcttatg ggaattattt
     1441 tagcatctgg actgggagac tggattgcaa gagttatatc tccacttgca ggtaatattg
     1501 gtggattatt gattatatca gtaatttgca cacttccatt cttatcacca atactaggac
     1561 ctggtgcagt tatagctcaa gtagtaggga ctcttgtagg tacacaaatt ggtcttggtg
     1621 caataccagc atatttagca cttccagctt tatttgcaat aaatgggcaa gctggatgtg
     1681 attttgtacc agtaggatta agtttgggag aagcagaacc agaaactgta gagtatggtg
     1741 ttccagcttt attttactct cgtttaataa ctggtccaat agctgttatt attgcatatg
     1801 gagttgcagt atttgcattg agataatatt taatgtcaat ataaggcttg gaattaatta
     1861 ataaaattgt aaatataaat ttaagctagt caagattttt tgttaatgaa agactagctt
     1921 ttatactatt aattagctgt aattatcgag ttagattaat actaaatttt cctttattaa
     1981 agtgaaattt tatgtattaa tttggtttca aatttgtact agaatgaaat caaggaggag
     2041 atacaaaatg agtgtaactg agaaaattcc ttctattgac cgcaagatat ttaagatatt
     2101 gattatgtgt agtaaaaaag agtttgtgag tattaactca atagcaaatg aattgaatgt
     2161 cacaactaga agtgtaagga cgtatataaa acaattgaat aaagatttag gtaatgatat
     2221 agcagtaatc aaatatataa agggtcaagg ctataaacta gaaataaaag atgagcagat
     2281 actcaataag attattgata tgaatagaaa aaatgtattt tcattaaatt caaaagaaga
     2341 tagagtagag tttatactaa attacttgat agaactagat ggatttatta cacttgatag
     2401 tttagctgat gagatgtgtg taggaagaac tactttagta aatgatttcc agtatgtaga
     2461 aaaagttttg gcttcatata atttaaactt gattaaaaaa cagaatacag gtatgaaatt
     2521 aaatggaaat gaacttgata ttagactgtt tatattaaat cagttgtata aaaactcaag
     2581 gaaagatttc agtaattcaa agtattttaa aggaatcaaa aaagaagaaa tcataaattt
     2641 agaagaaaaa ctattgactt tatttaaaaa aaataatttt tatgttacag atgaaatgtt
     2701 aagagaagtt attaactaca tagtagtttt agtctataga gtaaaagaag aaaagaaagt
     2761 aaaagattat gatgtaaagt ttgatttact taaatcatat gatgaatact ttattgctag
     2821 ggaaataaaa catataatat caggaatgtt tgaatgtact ttaaatgatg aagaaattat
     2881 ttatttaact ataccgttag ttagtggaaa tgcaccagca tcagaatgtg ctttaaatag
     2941 tagtagaatt agtaaaaaca ttgatgagtt gatggaaaat atatttaatc aaatatatgt
     3001 cgatatgggt atttttataa atgaggatga attaagagta ggtctaggat atcatcttag
     3061 ttttacttta aacaggctat tatttaatat aaagttaaaa aatgttttgt tagaagagat
     3121 taagcaaaat tatatacttc catttaaatt agcacaaata gcaggaaaag taatagagaa
     3181 aaagtataac ttagaagttt cagaagatga gattggatat atagcaatcc atttcagtgg
     3241 atatttagaa agaaatagca gtagattcta ttcaataaaa aaaatagcaa ttatatgtag
     3301 tacaggtcta ggaacagcaa aactattgaa aataagagta gaaaaactga taggaaataa
     3361 tcctaaaatt gatactcttt caagttttaa tttgagaaat attaacttag atgaatatga
     3421 tatagttttt acaactattg atttagacac atcaaatata aatacaatag tattaaaaat
     3481 aaatacaata tttgatgaaa acaaactaag agaacaatta aaaacagtat tgtgcctaag
     3541 agaagggaat attgacacta ctaattctac aaatttacta ataaataatt tgttagatga
     3601 agataaattt atgatattaa atgaaaagac tatacttgct tcattagaaa aaatgatgga
     3661 ccatcttatg gatttaggat gcattgatga taagtttaga aaaaatatag tcaatagaga
     3721 agaaaaatct cccacagtat ttgataaagg tttattattt cctcattctg taaatgaaaa
     3781 atcagataag tttttaatgg cagttggaat tttagaagaa ccaatagaat atgcaaatag
     3841 aagtataaaa ataattctta tgacaatgtt tccatgtgag aataagatag actcagactt
     3901 attagtaaaa atttatgaag aagttttaaa gataggccaa gatgtaaaat taacgaataa
     3961 gataagtaaa tgtagaactt ttttagaatt taaaaaggtg ttacttaaaa accttatata
     4021 actattgtac agcatttata gttgttatac agatttaaaa ataatgtgat ttttggaagg
     4081 tgtataaata tgaaattatt agtgatttta tttgttgctt atattttaaa tactatattt
     4141 ataatgaagc agaataaatt gtattttaaa actttaaata gagctaaaaa aaagggtgat
     4201 atagtgtcta caggcaaaaa gaaaagctat ttttctaaag gaagtatagc cattatatct
     4261 tcagattcag atgggtttat aaagtttgga gaaattttaa aaggacgtac agtaatggct
     4321 aaatttaaag agattgaaga aatagaagga ttagatatat atagtgcaga gcaaaaattt
     4381 aaagatgaag agagtattgt acaagctata aaatttataa aagaaaaaat aaatgtttct
     4441 tttaattaac tcaatataaa tataaattat caggaggaat gtcatgaaat atgaagttaa
     4501 gataagtggg ataggcagtt tagttaaaga gttaatggat gaaagcaatt gtcttattat
     4561 atatgatgaa accattaatg atgacgactt aaaggatata tctgtaattc attcaatagc
     4621 aacgttaaaa agtgatgttg aaataggaga tacacttact atagggaata gagattattg
     4681 tatagtttca gttggtgata ttgctcagaa gactcttaga gaaattgggc attgtacaat
     4741 caaatttgat ggaaaatgtg aagtaaattt accaggagaa atacatgtgg aaataggaaa
     4801 tccagatata actattggag atttaataac tatttcttaa tttgaaaata aaattattat
     4861 aacaggagga attggtatgt tagatttaaa cagaaaactt gcaaaattgg aagaggaagg
     4921 taaaaaaata aaagtagctc ttataggagc tgggcatatg ggaaatggaa tggtaagcca
     4981 aatggcaaat atgaaaggta ttgaagcttc tattgttgta gatataaatt tggagttggc
     5041 tcataaagct tttactgatg caggaataga tgaagaaatt atagataatg taacaaatga
     5101 aaaggatgca gaaataaaat tacaagaagg aaaagtattg acatgtaatg attttcttgt
     5161 ggcttgtaaa actaagtcaa tagatgtagt tatagatgca actggaggta ttgcaattgg
     5221 tgcagagata gcattaaact ctattttaaa taaaaaacat atagttatgt taaatgctga
     5281 aactgattgt gttgttggac caatacttaa aaaattagca gatgatgctg gagtaatatt
     5341 tacaggttct gcaggagatg agccaggtgc agtaatggaa ttatttgatt ttgcagatgc
     5401 aatggggttt gaagttagag tgataggtaa aggtaaaaat aataaattag atttagattg
     5461 taatcctgat acagttagag aagaagcaga aagaaaagga gcttcacctc atatgatagc
     5521 ttcttttaaa gaaggaacta aaacaatggt agaaatggct ttaatgtgta atgcaactgg
     5581 atttgtgcct gatgtaagag gtggtcatgg tattgaagct acagttaatg aagtacctaa
     5641 gaaatatgca ttaaaaagcg aaggtggagt attagataat tatggtgtag ttgattttgt
     5701 aaatggtata gcacctggag ttttcgtagt agtagcccat aaattaaaag cagttaatga
     5761 tgaactaaaa tatttaagta tgggtgatgg acctaattac atattatata gaccatatca
     5821 tttatgtagt ttagagactc ctttatcagc agctatggca gtcttagacc ataaggctac
     5881 aatagtacct aaagctggac tagtagcaga agttatgact atagctaaga aagatttgaa
     5941 aaaaggtgag tttatggatg gctatggagc atatacatgc tatggtacta tagaaaaata
     6001 cgatgtagca aaggctatga atgctgtacc tattggtttg ataagtaaaa agactaaagt
     6061 tgtaaaagat attaaaaaag gtgaagttat aacttacgat atggtagaga tagaaaaaga
     6121 tactacttta tatcatttaa gacaattgca agaaaaaata tttgggtaag atttgatggt
     6181 aatataaata gatattagta aaaagggttg atggttattt ttaaatcaac ccttttaaat
     6241 ttttatatat tcttgttagg atactttctt taaaagtata ttataggtta tttgtagtcg
     6301 taaaaattat tttgaattag ttctgcaatt ttatattcac attgaaataa tattatta
//
LOCUS       DAEMGG010000176         6315 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEMGG010000176 DAEMGG010000000
VERSION     DAEMGG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.177"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..235
                     /locus_tag="KPK24_003505"
     CDS             <1..235
                     /locus_tag="KPK24_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301139.1"
                     /translation="VTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIEDVL
                     YIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            383..574
                     /locus_tag="KPK24_003506"
     CDS             383..574
                     /locus_tag="KPK24_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG2301140.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            858..3359
                     /locus_tag="KPK24_003507"
     CDS             858..3359
                     /locus_tag="KPK24_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG2301141.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQSMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            3519..4187
                     /locus_tag="KPK24_003508"
     CDS             3519..4187
                     /locus_tag="KPK24_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2301142.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            4171..5448
                     /locus_tag="KPK24_003509"
     CDS             4171..5448
                     /locus_tag="KPK24_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG2301143.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5849..6070
                     /locus_tag="KPK24_003510"
     CDS             5849..6070
                     /locus_tag="KPK24_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301144.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         2685 a          609 c         1100 g         1921 t
ORIGIN      
        1 agtaaccaaa ataataatta aatattttgc aagtgaaagt gatacttttg taaaagaaga
       61 agtttgtaat tttgaagaac ttcttaagtg gtctcataaa tttattgaag gtaaaaagat
      121 agaagatgtt ttgtatataa agtctaaaga aaactataaa gtaaatgtga ttaagtcttt
      181 agaaattaag gaattggaaa tagtgagaaa acaacagata ccaccgtaca tgtagtatta
      241 ataatattta aaattaatat gtaatactca caataggata taaatggtat atatggtaat
      301 aatttatatt aaaaaaactt ttataaagtt tatatttggg tataaaagta ttaattagac
      361 attaattagg aggaacttaa atatgataag agtatgccca aactgttcaa atgtggatgt
      421 aaataagcta aaaactttgg ttggagaaga aaatgtaaag actggttgta taggtcagtg
      481 tagagcattt aaaaaagaag ctgttggaat aatagacaat gagcttgaaa taaaggaaac
      541 agaagcagag ttttttgaag catgtaaaga ataaatttta aataaattat aataaataga
      601 gcatctaaat tgtataagca ttaaacaatt tagatgctct atttttatat atacatttaa
      661 ttatgagtat acttttctat taaatacaaa taaatataac taatacattt atttattaaa
      721 aaaatattga tttaatatac ctataggggg tataataaaa atatagattt ctaaataagt
      781 aatttatcaa tattacatat caaaataaaa cgttacaaat tagctgaaaa tatattgaaa
      841 gaaaggaaga ttttattatg agttcaaata aagtggtaga aagttataaa attacaggta
      901 tgacatgtgc agcttgtgca aaggcagtag agagagtaac taagaagatg gatggagtat
      961 atgaccaaag tgtaaatata gctactgaaa aacttaaaat agaatatgat aattctaagg
     1021 ttaattttga tgatataaag caggttgtag aaaaagcagg atatggaatt ataaaggaag
     1081 aaagtaataa aaaaattgat atgaaaattg atggaatgac atgtgcagct tgtgcaaaag
     1141 ctgtagagag agtggtaaaa aaattggatg gagtagaaag tattagtgtg aatattgcaa
     1201 cagataaagc taatatagac tatgacccat caaaagtaaa attatctcaa ataaaagcag
     1261 ctattgaaaa agctggatat aaaccaatag aagaggtaag aaataaggtt gatgtggatg
     1321 aagataaatt gagaaaagaa agagaaatga agtctttatt tgttaaattt atagttgcaa
     1381 tagtatttgc agtacctctg ttttatatag caatgggacc tatgataata aagcctatag
     1441 ggccatggcc tttgccagaa attataaatc ctatgacaaa tacttttaat tatgcattaa
     1501 ttcagttaat tttagtaata ccagtaatga tagctggtta taaattttat ataaatgggt
     1561 ttaaatcact tttttcttta agccctaata tggattcttt agttgcaata ggaacattag
     1621 ccgcattttt atatagtcta tatacgacac tacaaatagc caatggacaa attcaaagta
     1681 tgcaccatca tcagttgtat tatgaaagtg caggaataat aatagcactt atcttacttg
     1741 gtaaatattt agaatcaaaa tcaaaaggaa aaacatcaga agcaattaaa aagcttatgg
     1801 ggctacaacc taaaactgca atagtgttag ttgatggtaa agaagttgaa actcctatag
     1861 aagaagtaga gataggagat atactattag taaaaccagg aactaaaata cccgtggatg
     1921 gagttgtaat agaaggatat acatcagttg atgaatcaat gcttacaggt gaaagtatac
     1981 cagtagaaaa gaatgttgga agtaaggtga caggagctag tattaataaa aatggtgtta
     2041 ttaagtttaa ggctgaaaaa attggtggag atactgctct tgcccaaata ataaagttag
     2101 ttgaagatgc acaaggtaca aaagcaccta ttgctaaact tgccgatact gtatctggat
     2161 attttgtacc aatagtaatt gcaatagctg tagttgcatc attattgtgg tttttaatag
     2221 gaggaaaaga tatagtattt gtacttacta tatttatatc agtacttgta attgcttgtc
     2281 cttgtgcatt aggattagca acaccaactg ctataatggt tgggactgga aagggcgcag
     2341 aaaatggaat cttgataaaa ggtggagagg cattagaatc tgcacataaa gtaaatacag
     2401 tgatatttga taaaacaggt actataacag aaggaaagcc aaaagtaaca gatatagtat
     2461 taaataataa tgtaaaagaa gaatatctta tcaagattgc ttcaagtgca gaaaaaggtt
     2521 cagaacatcc tcttggagaa gcaatagtta agtatggaga agagaagaat ataaaatttg
     2581 aaaaagttga taattttaaa gctataccag gtgctggtat acaagttact attaatgatg
     2641 aaagtatatt acttggaaat agaaagctta tgaatgacaa taatataaag cttggagatt
     2701 tagaagaaaa atcaaatata ttggcaagcc aaggtaaaac acctatgtat atagctgtag
     2761 atggaaattt atcaggtata atagcagttg cagatgtagt caaagaaagt agtaaaaaag
     2821 caatagaaat acttcatgat atgggaataa aagttgcaat ggtaactgga gataatgcga
     2881 aaactgcaaa tgctatagca aatcaagtag gtatagatat ggttttggca gaggttttac
     2941 cagaagataa gtcaaaagaa gttgaaaaac tacaaaatca aggaaagttt gttgcaatgg
     3001 taggggatgg aataaatgat gccccagctc ttgcaaaagc agatataggg atagcaatag
     3061 gaagtggaac ggatgtagcc attgaatctg cggatatagt acttatgaag agtgatttaa
     3121 ttgatgttcc aacagcaatt aaacttagtc atgaaactat aaaaaatata aaacaaaacc
     3181 tattttgggc atttggatat aatacaattg gtatacctgt agcagcagga atattatatg
     3241 tatttggagg acctttatta aacccaatga ttgcagcagc agcaatgagc ttaagttcag
     3301 tttcagtcgt atcaaatgca cttagactta aaaacttcaa ggcatataag agagattaga
     3361 ttaataaatt aaataaaatg tatccaaaag agctatcttt aaattaaaag gattaattta
     3421 caaattaaga tagctctttt ttcatgtaaa attcacaaca ataagataaa atataagtaa
     3481 tatacgaaat tttaaataaa atagaaagga ttgatagaat gaattcgtca atacttgtaa
     3541 ttgaagatga ttcaaatata caagagttaa tatcagaatt tttaagtgca gaaggttatc
     3601 aagttgatac agcaaatgat gggttggaag gtatacaaaa gtttaagcaa ggaagttatg
     3661 atttagtaat attagatata atgatgccaa acttagatgg ttatggagtt tgtaaaatga
     3721 taagaaagtc atcaagcgtt ccaattatat ttttaactgc tttaaatgat gaaggagacc
     3781 aacttaaagg gtttgactta gaatgtgatg attatataac aaaaccattt tcatttaatc
     3841 ttcttataaa aagagtagaa gcaattttaa ggcgaagtaa caagactata aatgacaaat
     3901 ttatagtttt tgaaaaatta aaattagatt taaacacata tattgcagag atagatggag
     3961 aacctataga acttacacta aaagagttta atatattaaa agctttgata gaaaagtatc
     4021 cacaagttat aactagggaa ggtcttttgg atagtatatg gggttatgac tattatggag
     4081 atactaggat tgttgatgca catataaaaa atataagaaa aaaaatatca ttaccatata
     4141 ttaaaactgt aaaaggaata ggatatacac ttgagaagga tatttgataa gtgggaaaag
     4201 ttaagtataa agtataagtt attttcaata acgacatcat tattaatagc attagcttta
     4261 ataatatatc taattttata ttttttattg ccatcatatt atcatgagta taaaattgag
     4321 tctcttcaag aaagtttgaa atctttagta gatagttcaa ttcatttcga tacatatact
     4381 ttagaagaaa gactttatta tatggctaaa gaccaaaatt tggctatttt attaaaagat
     4441 aaccaaggta aaattgtata tggtaagaat gaagtagtta ttttaagata tagtaagtac
     4501 atgattaata gtcttgagga tgagtataga acttctatac ctatatatac taaagatgca
     4561 aaagatggtc catatacttt agaactcgtt atgccattac aaccaattga cgaagcaaac
     4621 gaagttataa gaaaactaat gccatacatc atatcaatag caatattgat agcgattatt
     4681 ggagcatata tatattcaat tgtaataaca aagcctctta taaacataat agagagtgag
     4741 agagaacagg aatatagaag gaaggatttt gttgctacaa tatcacatga actaaaaact
     4801 ccaataacca taataagtgg tcaaattgaa ggtatgattt atagtgtagg taagtataag
     4861 gatagggata cttatctgaa aaaatcgtat gagtgtacac aagaattaaa agatttagta
     4921 aatgaaatga tagaggtttc aaaatcagaa atacttgaaa aagatttgaa attggtatca
     4981 ataaatataa gtgaattatt aaatagactt gtaaagagac aagtattttt aatagaagaa
     5041 aaacatatga aaactatttt aaaaatagaa gaaaatttgg aaattaaagc agaccaagag
     5101 agaataacta aggcaatcaa taatataata aataatgcca taaaatattc tcctgaagaa
     5161 tcagagctta taattagact ctatgataaa aataaacgaa taagtaaaaa gacttctaat
     5221 caaagagtgg ttttagaaat tgaaaatact ggtgtaacta tcgagaaacg ttatttagaa
     5281 gaaatattta atccatttta tagaatagaa aaatctcgtt cgagaaaaac tggaggaagt
     5341 ggacttggac tttatatagt cagccaaatt tttaaaagtc atgggtttga ttatagcata
     5401 aaaaacaaag aaaactctgt agtattcaca gtagaattta aaaattaatt acttattata
     5461 aagaatatat aactttcaat aatagttcat tttaaaatca caaaaaattc ataatgaaac
     5521 tgtattatat aaatataaag aagcttaaaa ccataaaata caccaactat aatgatgaga
     5581 tttccttaca tcctccatat aagtcatcat tatagtaacc caacaattaa gttactatga
     5641 atttaaaaaa taacaccgag catataatct atgcttagtg ttatttttat tttgaaataa
     5701 aaaatattta atttattgaa atgtatagat ttagatagtt taaactatat ttaagactta
     5761 aaaattataa ttgatattac aaataaatat atgtactttg ggaaaaataa atataggaag
     5821 tattatattt acagaggagg tagttttgat ggatagatta aaaagagata tgaaaaatat
     5881 gaaagaagat ttaaaagaag gtgttaaaaa gacagcttat gttatgaaaa atacagctga
     5941 agatttgaaa gatgatgcac aaggtgctat ggtaaactta gaaatgaaaa aagatgagat
     6001 gatggaaaga tatgaacaaa agaaattttc taaagaatta gagaagcaaa tgaagaatga
     6061 aatgaaataa gcttttattt atgaataaca aattattgta ttcatataaa aagtagccag
     6121 taatattaaa tagtttattt aaggaaatag actttctaaa aataactgta ttagatttag
     6181 aaagtctatt tcaaattagt tttataaata gacataagga taaagtagat gttatagaac
     6241 tagattttaa aaaatagata ctaaggataa aataaatatt atagaaatat gtttttaaga
     6301 atagttatca aataa
//
LOCUS       DAEMGG010000177         6311 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEMGG010000177 DAEMGG010000000
VERSION     DAEMGG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.178"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            202..645
                     /locus_tag="KPK24_003511"
     CDS             202..645
                     /locus_tag="KPK24_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2301145.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            676..2493
                     /gene="ftsH"
                     /locus_tag="KPK24_003512"
     CDS             676..2493
                     /gene="ftsH"
                     /locus_tag="KPK24_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG2301146.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            2671..5373
                     /locus_tag="KPK24_003513"
     CDS             2671..5373
                     /locus_tag="KPK24_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG2301147.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            5407..6105
                     /locus_tag="KPK24_003514"
     CDS             5407..6105
                     /locus_tag="KPK24_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2301148.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         2493 a          707 c         1151 g         1960 t
ORIGIN      
        1 taaattttat taaaaatatc ctgtataaaa ttaaatatca ataagataac aaaatttcaa
       61 ttgaagttac aatattgtaa ttgacaataa atacttaata ctataatatt taatacatta
      121 aacattatag tgttaagtat ttattacagt atgcacttca gcctaaattt gaaataaagt
      181 attttcgtag ggagcgtttg tatggatgag atgagtttta agaaagagct actagagttg
      241 actagagata taaatatgaa atttacaact ttattaagtg atttttatca gccacttggt
      301 attacagcag tgcaagcttt gattttatct gagttatgtg aacatggaga gaaaaaaata
      361 agcgatttag ggaagaactt aaacatgaca aatagtaatg tatcagtaat ctgtcaaagg
      421 cttgaaaaaa atggcttttt aaatcgcata agagatatag aagaccaacg aattgtaaaa
      481 gtaaaagtta caaataaatc attggatata caagaacata tttcaagtag tatttttgat
      541 agttattttg aaaatatgac cagtgaaaaa ttgcaagata tggaagatat aatagaaggt
      601 ttagagaaat taaataaact tttaacttac attgattgca aataacataa aaataattta
      661 aaggagaata aaattatgga tgaacataaa ccccaaaaaa ggtcagcact ctattattat
      721 acagttataa taatagtagt aatgttttta aatgctttaa tatttccaag tttaatggat
      781 aaaaaaatgg ttgagattga ctatggaaag tttctcacaa tgttggatac aggaaaggtt
      841 aaatctgttg agatagaaaa taatagaata gcaataaatc catctagtag caaggataag
      901 aatatataca taactggtag aatggatgac ccagaattgg tagatagact aaaaaatgca
      961 aaagtagaat ttactaaggt tattcctaag gaaaattcac ctcttttaag tattttactt
     1021 acatggataa tacctatagg tattcttatg ctatttggta acttaatgat gaagtctatg
     1081 caaaagcgaa tgggtggtaa tgctatgcaa tttggaaaaa gcaacgccaa aatgtatgta
     1141 tctgctcaaa gtggaaaaag atttaatgat gtagctggtc aagatgaggc aaaagaagct
     1201 ttaactgaaa tagtagattt cttacacaat ccagaaaagt ataaaaagat aggagcacaa
     1261 atgcctaaag gagcactttt agtaggacct ccaggtacag gaaaaacact ccttgcaaaa
     1321 gctgtagcag gtgaagcaaa tgtaccattt ttctcaatat ctggttctga gtttgttgag
     1381 atgtttgtag gaatgggagc ttctaggatt cgtgatttat ttaaacaagc aaaagaaaaa
     1441 gctccatgta tagtgtttat agatgagatt gatactattg gtaaaaagcg tgataatgga
     1501 agtggattag gtggaaatga tgaacgtgaa caaactttaa atcagttgtt aagtgagatg
     1561 gatggttttg atggtggaat tggagtagta atacttgctg ctacaaatag acctgacagt
     1621 cttgacaaag cattacttcg tccaggaaga tttgatagac gtataccagt tgaacttcca
     1681 gaccttggag gacgtgaagc tatattaaag gtacatgctc aaaatgtaaa tgtaagctca
     1741 gatatagatt ataatgcaat tgcaaggtct acatcaggag catcaggtgc agaacttgca
     1801 aatattgtaa acgaagcagc acttttagca gttaagtctg gcagagatat agtagaacaa
     1861 caggattttg atgaatctgt tgaaactgta attgctgggt atcaacgtaa aggagctgta
     1921 atttcagata aggaaaagaa aattatagca tatcatgaaa taggtcatgc acttgttgct
     1981 gctatgcaaa aacattctgc acctgtccat aagattacta taatacctag aacttcagga
     2041 gcattgggat atactatgca agttgctgaa gatgagagtg tacttatgtc taaagaagaa
     2101 gcaattgata aaataactac ttttacaggt ggtcgtgctg ctgaagaggt tatctttaac
     2161 acttgtacat caggagcatc aaatgatata gaacaagcta ctaaaattgc aagagctatg
     2221 gttactcgcc ttggtatgag tgaagagttt gatatggtcg ctcttgaaac tgtaagcaat
     2281 caatatttag gaggagatac ttctcttgct tgttcttccg acactgctgc taaaatagat
     2341 gaagaggtat ttaaaatagt aaaggcttgt cacaaaaatg caattgaaat cttagaaaat
     2401 aatattgata aattgcatga acttgctaat tatttattag aaaaagaaac tattacaggt
     2461 gatgagttta tggaaatact taacttaaac taaaatgatt atacacaaga taaaaatgcc
     2521 tattgttagg catttttatt ttacaactaa attttttaaa ttcatattta aatctttaca
     2581 taatcttaat cattaaaaat acatcttaac tacaatttta tatcggaatt gatattataa
     2641 tagtaatgaa tggtgtaagg aggtttttta atggatagac ctaatcctga tattctatta
     2701 gaaaaaatta aaaatgaaga agaaaaacta agtagaggtc aattaaaaat attctttggt
     2761 tatgcagctg gagtaggaaa aacctactct atgttggaat ctgcacaaaa tttaaaaaaa
     2821 gtgggtgtag atgtagtagt tggatatatt gaaccacaca ctagaccaga aacattggct
     2881 ttacttgatg gcttggaaat tcttcctgta aaggagatag aatataaatc tataaaacta
     2941 aaagaatttg atttagatgc tgctttagca agaaaaccag aagttatatt agtagatgaa
     3001 tttgcccatt ctaatgttgc tgggttaagg catacaaaaa gatggcaaga tatagaagaa
     3061 ttgctgttag caggcataaa tgtctacact actgtaaatg tacaacattt agaaagtcta
     3121 aatgacattg ttgagataat tacaaacgta tcagttaaag agactatacc agataagttt
     3181 ttggatacaa atacacagtt agaacttata gatgttgaac ctgatgtact tcttgaaagg
     3241 ttcaatgaag gtaagatata taagaaagaa caagctacta gagcaaaaaa taattttttt
     3301 ataaaagata acctggttgc acttagagaa atcgcactta gaaaaactgc agagagagtt
     3361 aataaagaag ttcaaatgac cagactatca aagggtgatg taactgtaat acctacatca
     3421 gatacacttc ttgcatgtat aagtccatct ccatcttcag caaaagttat tagaactgca
     3481 tccagaatat cagacagtac atttgcaaaa tggatagctt tatatgttga aactccaaac
     3541 acagcaagac ttccatttga tgaacaaaaa caactacaaa ataacctaaa attagctaag
     3601 aaattaggtg gagaaataat tgttttacat ggtgaaaata taatagagca aatacttaga
     3661 attgcaaagc tgagaaatgt aacaaaaatt gtaataggta gaaatcattc aagtaataaa
     3721 agatttagta aaagatttaa aaaagatata gttgacaaat taattgatga agttgattat
     3781 atagatatac atataattcc atataaattt gcctcagatg ttaaatacaa acctaaaaag
     3841 gataaatcat caataaaatc taaatttaaa attagcaaag ttgattttat aaaacttata
     3901 tttattactt tgatgataag tattttagca tatgcagtac aaagcatggg atttataaga
     3961 gaaaatatat tgttaatata tatgttgggt gtagttttag tttctatgtg gactaaaggg
     4021 tatagtacag gtataataag ttctgtattt aatatagttc ttttaaatta ttttttcaca
     4081 gcaccattat atacattatc tatagcagat tcaaactata tagtaacact ggttgtattc
     4141 tcaatagtag gtataataac tagcactctt acgtctaaga tacagcatga agcagaaact
     4201 gctgctaaaa gagaagaaaa tacaaaaatg atatatcaaa taattaaggg atttttaaga
     4261 ttatcaaata aagatgatat agtaaacaaa ggtatagaac ttttgtctct tagcttaagt
     4321 agagatgtaa tttgttattt aaatgtagat aagcataata aatcaaagct ttacaaaaaa
     4381 aatactaata ataaggataa aaatgatttg gatagtgaag atgaaaaagc tgttgcaagt
     4441 tgggttttat ccaattcaac agtagcagga aatgacacgg atacacttcc tggttcaaag
     4501 ggatactata taccaattgt aggaatgaat ttaactttag gagtagtggg aatatcatgt
     4561 attgattcaa aattggatac agaagatata agcttaattg aaacaataat tgctcaaatg
     4621 gcaatagcgc ttgatagaga aatcttgtca gaagctaaag aaaataccaa tcttgagatt
     4681 gaaagggaaa gattaagaag taatctttta agagcagttt cgcatgacct tagaactcca
     4741 ctagcaggaa tatctggtgc agtaagtaca attataaaaa acaagggtac tataggtcaa
     4801 gatataatag atgaattact tagtggtgta tatgaagata ctcagtggtt aataagactt
     4861 gttgaaaatt tacttagtat gaccaagata gatgaaggga aattggaagt taaaaaacat
     4921 tctgaactgg ttgaggaaat tatatcagaa gcgttacaaa aaataaaaaa aagaattgaa
     4981 aatgcactta tagatattaa tataccagaa cagatattat ttgttcctat ggatgcaaaa
     5041 ttaatagaac aagttttaat taatttaata gataattctt tgaaatattc aaaggaagat
     5101 tgtaagatag agataaatgt atatgagaaa gatgattatg tttggtttga agtatctgat
     5161 aatggtcctg gtatatcaaa ggaacttaaa aagcatatct ttgacagatt ttttactgga
     5221 gaagaaggag ctaaagattc cagaaagggt gttggacttg gattatcaat ttgtaaatct
     5281 ataattcaag ctcataaagg tgaaattatg gtagaaaaca ataaagataa gggttcaact
     5341 tttaagttta gtttgcctaa ggaaaatgaa tagttttaaa ttttaaaatt agttttggag
     5401 gaagaaatga gtaaaaaact tatattgtta gtagaggatg ataagacaat aagaaaattt
     5461 atatctactg cacttttaac acaggattat gatgtcaagg aggctataac tggaaaagaa
     5521 ggtatatcta tagctgtctc atattctcca gatgttgttc ttttagattt aggcttagaa
     5581 gatatggatg gaatagaggt aataaaagct atcagacaat tttcaaatat accaataatt
     5641 gtagtttcag ctagagagca agatagagac aaggtagaag tatttgatgc aggggcagat
     5701 gattacttaa caaaaccatt ctccatagta gagttattag caagagtaag agtagcattt
     5761 agacattcac aagtagaagc tcaacaaaaa gatgatgtta aatctacgtt tgaagtagat
     5821 aaattattga ttgattttga taaaagaaaa gttattgttg atgatgttga agtccatttg
     5881 acacctattg aatataatat tctttcatta cttgcaaagt atcatggaaa agtgcttact
     5941 cataatttta taataaaaga aatatggggt agtgttatag ggaatgagac taagtcttta
     6001 agggtcttta tggcaacgtt aagacgaaaa atagaaaaac aacctgcaaa cccaagatat
     6061 ataattacag aagttggtgt aggatacaga ttaaatgatg aatagttgta atatattaat
     6121 tattagatta tgtgcttatt aatatatatt aaaattatat agtttagtta aaataagaat
     6181 gaagtgtatg tgcttgttgt cagtttagca catgcatttt attttttagc ttttttagta
     6241 tcaataattt tttagtatca ataatatgaa atatgtttta aaataagtat atagtgtaat
     6301 ctgtaataaa a
//
LOCUS       DAEMGG010000178         6191 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEMGG010000178 DAEMGG010000000
VERSION     DAEMGG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.179"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            119..769
                     /locus_tag="KPK24_003515"
     CDS             119..769
                     /locus_tag="KPK24_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG2301149.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(909..2285)
                     /locus_tag="KPK24_003516"
     CDS             complement(909..2285)
                     /locus_tag="KPK24_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2301150.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2301..4214)
                     /locus_tag="KPK24_003517"
     CDS             complement(2301..4214)
                     /locus_tag="KPK24_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2301151.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4350..4982
                     /locus_tag="KPK24_003518"
     CDS             4350..4982
                     /locus_tag="KPK24_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG2301152.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5121..5660
                     /locus_tag="KPK24_003519"
     CDS             5121..5660
                     /locus_tag="KPK24_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG2301153.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(5836..6006)
                     /locus_tag="KPK24_003520"
     CDS             complement(5836..6006)
                     /locus_tag="KPK24_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2301154.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2295 a          857 c          861 g         2178 t
ORIGIN      
        1 gtagaatgga tttgtcaagt ttaatttagt tgtaattcta tcttttatta aaaataacat
       61 aaattaaaac atataaataa aatattttat acaatatatt gtaaaggagg taactttgat
      121 gaaagcagaa aaaacaagta tatatccaat tattaaaaat aatttaaaca aaatatcaat
      181 tgttgaatat aaaaagggtc aaagatttgt agcatcagat aaagacttac aagaagtgtt
      241 ctttataata gagggagttg cattagtgga gtgtactact agaggaggaa ataaatttct
      301 tgttgacatt gtacctgaaa atgaatttat aggcaaaatt agttacatat atgaacataa
      361 tttgaaatgt gatatatttg caaagactaa tataaaatta tttcgatttg aaaaaaatgt
      421 atttgaagag ttttatctca aaccagattt tattgcacta tttcacagaa aatgtacaag
      481 aagaatatat gaattatata aaaccagaat ggtaagagaa ctattttctt gcacagaagt
      541 tattgcctat tgtatatcaa ataaacaaga aaataatgtc tgcaatataa aagctattcg
      601 taatttcagt gaaatctatt caattagtag aaaaagtaga taccattctc tgctaaagtt
      661 aactgaaaag gagatagtaa aaaaaatggg taatacatat gaaattttaa actatgatga
      721 attacataaa ttggcatttg aagtaaaaga atttttggaa gatgagtaaa aattgttact
      781 taatcaaaat aaagtataat aatttctgta aaaaatagaa atagtctcac tgaaaatcaa
      841 aaatacttta gttattttca atgagactgt ttagaaatat tgtatactta taatgtatag
      901 tataattatt aatcaatttc ctttactata ttaatagccc attttttaga taatgctcta
      961 ggaacaccaa atataaactt aaccacttct tcactataag tgagcaaaac gagtacaaat
     1021 ataggtgcac ctttaaatgc agctataaaa gttagaggaa tagaagctaa ccacatacat
     1081 cctaattcta aaaatagagc atatttagta tctcctccac ttcttaaaat accaattata
     1141 accaatgtat taaaagattt aagagccatt agtattccca ttataaagaa tatcttagtt
     1201 atatcagaag ctaagctatc agaaacacta aacattttta ataataatgg tatatttaaa
     1261 attaaaataa ttccaagtat taaaccagct gaaaatacaa gtatagaaaa ctttttagca
     1321 tattcaatgg ctcttttaat atggtcagct ccaagttcat ttccaagcat aatagatgca
     1381 ccagatgcaa tacatacagc tgtcattatg aagaaattac ctgtactggt tgctatctgg
     1441 ctagctgcta tagcagaagt accagctctt gcatatgcaa cagaatacag gacagttcca
     1501 attgcccata cagagtcatt tacaaatata ggaaagcttt ttgatgaaaa agattttata
     1561 aaaattttat caatagcttt tagatttttt agaccaaact ttaatatata gtctttttta
     1621 taaaggtaaa cataagtaat cattaaaatt aattcacata ttctagcaat aacagtagct
     1681 agagcagcac cttttacacc caatgcagga agaccgaagt taccaaatat aaatccataa
     1741 tttaaaataa catttgttac aagtgcaaat gcactacaaa tcatacctaa cttaggattt
     1801 ctaacacctc ttgaaccagt actaaataca gtacttacag caattaatgg ataactaaat
     1861 actgcaatac taaagtaatc tatacaaagt tttaccacct cagaatcata cgaaaatatg
     1921 tgtataattg gctttggtgt tagtaaagca ggaataacaa atacaaaact taaaacaatt
     1981 gccaatacac aagtaagacc tgtgactttt cttatattac tcacatcctt ctttccataa
     2041 aactgtgaaa taaaaactcc agcacctcct gtgataccag ataaggacat attaaaaaga
     2101 aagaaaaatt ggtttgcaac tccaactgaa gctacagatg tttcccccaa actacttatc
     2161 ataattgtat ctattacatt tacagaagta gaaattaagt tctgtatgat aataggtata
     2221 cataaagata gaagtatttt ataaaatctt ttattttcgt cttttaaaga aaatagttct
     2281 tgcaaataat cctctccttt ttacataaat tcttcccact catcatatag agaggcaagg
     2341 ttattttcta aattaatttt ttcttggcta acttctttag ctttatctgg attagagtat
     2401 acttcttctt gacataaaag tatatctaat ttttcaattt caatctctaa cttttcaatt
     2461 tctttttcta tattttgtct ttttattcta tttttctttt ctatttctct ttgttctctc
     2521 tcttttcttt tttcatcttt tatttgagtt ctagtttttt cttctttttc ttctataata
     2581 ctcatctctt ggatttgcct ttttttatct atataataat cataatttcc cagatattca
     2641 gtaattccat tttcatctaa aactaaaatt ttatcaacaa cagtatttag aaagtatcta
     2701 tcatgtgata ttgtaaatat agtcccatca taattagtta aggcctcttc aaggacttcc
     2761 ttagaatcaa tatctaggtg attagtaggc tcatctaata acaataagtt tgcatttgat
     2821 agtataagtt taagtatggc aactctagct ctttctccac cactcaatgt agaaatcttc
     2881 ttaaaaactt cttcatctac aaataaaaat gcccctaaca tatttctcaa agtagtttga
     2941 gtaagatgag tattattttc ccaaatctca tcaattattg tgttatctaa attaagagtt
     3001 ttttgttcct gatggaagta ggaaacatgt acattagttc caaatttaat attgccacta
     3061 agaggttgta gttcattagt tattatttta aataatgtag atttgccaac accatttgca
     3121 cctataagtg caactttttc ccctctatat atatctaaat ccaagtcttt aaaaagtatt
     3181 ctttctccat aacccataga tacatctcta atttgaagaa catcattacc actctggaca
     3241 gaaggcgtaa attgtatacg agcttttttc ctataagctt ctggtttatc taaaacatct
     3301 actttgtcta atgctttttc tttacttctt gcacgcttta ggtgtttttc tctaccataa
     3361 gcttttagtc tttcaattga ttcttcttgc ttttttattt ctttttgttg gtcttcaaat
     3421 ttcttaagct ctagttcttt ttctatttta gataactcaa caaatttaga gtagttacca
     3481 ttatatactt taagcttttt attgtgaact tcaaaaattc tatttacgga ttggtctaag
     3541 aaatatctat catgagatat taacatgaca gtccctttat attgttttaa aaatacttct
     3601 aaccattcta ttgcttcaga gtctaagtgg ttagtaggtt catcaagtaa caatagagtt
     3661 ggtttcttaa gaagtaattt tcctaaaaga actctagttt tttcaccacc agaaagtatg
     3721 cttattggct tatccatatc attgtcacta aatcctaatc cctttagaac tcctttggct
     3781 tcagatttat atccatatcc atttaaatct gaaaataatt ctagtttatg tgaatattca
     3841 tccatgattt tttctataat tggagagttt gtttttgtac tttcttcaga tattttaacc
     3901 tccaattctc ttaaatagga ttccatttct atcaaatttt taaaaacttc tagtacttct
     3961 tccaaaatag tattatccga ataaaaattg gtattttgtt ctaaatatcc gatttcacaa
     4021 tctttagaag tgtaaatatc tccactatca tatccataaa ttcctgaaat tattttaaat
     4081 aaagtagttt ttccagtacc attaacaccg attataccaa ttttatcgcc ttcatttact
     4141 gtaaaactaa tattttctaa tatagaatct ataccaaaac ttttatttaa gttattacat
     4201 gacaaaacaa tcataaattt ctacctcctt gaactatcta aaattataat gttttgatat
     4261 aatataatca aggttatatg tattagtttt gaaaaagaaa tattttattg tgaatcaaac
     4321 aaaaaagagg acacttttag gaggtgaaga tgttgggaaa taaaaatata tcaatggcag
     4381 ttataagaag gctcccaaaa tatcatagat atcttggaga cttattagat agggatatac
     4441 aaagaatatc ttctaaagaa ttgagtgata taatagggtt taccgcttct caaataagac
     4501 aagatttaaa caactttggt ggatttggac aacaaggata tggttataat gtagaagctc
     4561 ttcatactga gataggtaaa attcttgggt tggatcgacc atacaacgca gttcttgtag
     4621 gagcaggtaa cttaggacaa gctatagcca attatgcagg atttagaaaa gctggattcg
     4681 agataaaagc tttatttgat gcaaatccta gaatgatagg tttaaagata agagagtttg
     4741 aagtattaga ttcagatact ttagaagact ttataaaaaa caataatata gatattgctg
     4801 tattatgtat acctaaaaat ggagcacaag aagttattaa tagagttgta aaagctggaa
     4861 tcaaaggtgt atggaatttt gcacctttag atttagaagt tccgaaaggt gttatagttg
     4921 aaaatgtaaa cttaacagaa agtttattta ccttatcgta tttaatgaaa gaaggaaagt
     4981 agacaactta aacatattaa taatgagcag ttgtaaagag tattgagaaa gtcagaagat
     5041 ttaatattaa atcctctggc tttttgaaat attaggataa aaatttaaat aatgtatgga
     5101 aataaacaat attaaggaga gtggtttttg tggaggaatt atggaaaaag gcaattgaat
     5161 ttcatggtca tgaatgtcca gggttagcta taggatttaa agcagcactt gcagcaaaaa
     5221 aatatttagg atgtaaacag tcaggagatg aggaaattgt atgtatttct gaaaatgatg
     5281 cttgtggaat agatggtata caagtagtgc taagttgcac aatgggtaag ggaaatttac
     5341 tacttaggat gactggaaaa tcagtatatc atttctttaa tagagaaaat ggaaaatcta
     5401 taaggatata tgtaaagtca aaaccatttg agatggatag agaaggatac aaaagatatt
     5461 tattagaaag tgattttgaa gaaatatttg agattacaga tactaagtta agacttcctg
     5521 aaaaagcaag aatatttaaa tcccttaaat gtgaagtgtg tggtgaattt gcatctgagc
     5581 aatatataag gattgaagaa ggaaagaaag tatgtcttga ttgttttgat aaatatgaaa
     5641 gattttatga agagtattaa aattataaat tagattaaag ttaaaagtat tagatatgtg
     5701 ctaacagtat tttaagatga attaggtagc aacataataa agtatataaa taatattaat
     5761 tgaatcatac ataattaaaa agaggctgat tagtatttat aaaataaaat cagtctcttt
     5821 atgaataacg aattattatt ctggttgagg tgcatctaca ggacaagctc ctgcacaaga
     5881 accacagtct atacaagaac cagcatctat aacatatgcg tcgtctccag ctgatataca
     5941 gctaacaggg cattcagcct cacaagcacc acagcttata caagcgtcag taattttata
     6001 tgccattttg aaatcctccc ttttatatgt attgtaattc aataacatta taacaaatat
     6061 tattgaatat aacaattaaa ttaattctac aacaaaaact ttattatgat attcttgttt
     6121 taaagtttta caataaagtt taatctgctt atatttttat ttattttaaa gaagtttatt
     6181 ttaaaaataa a
//
LOCUS       DAEMGG010000179         6064 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEMGG010000179 DAEMGG010000000
VERSION     DAEMGG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.180"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(509..2644)
                     /gene="rnr"
                     /locus_tag="KPK24_003521"
     CDS             complement(509..2644)
                     /gene="rnr"
                     /locus_tag="KPK24_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG2301155.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3237..3851
                     /locus_tag="KPK24_003522"
     CDS             3237..3851
                     /locus_tag="KPK24_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG2301156.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTNRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            3869..4231
                     /locus_tag="KPK24_003523"
     CDS             3869..4231
                     /locus_tag="KPK24_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301157.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4337..4834
                     /locus_tag="KPK24_003524"
     CDS             4337..4834
                     /locus_tag="KPK24_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG2301158.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
     gene            5638..>6064
                     /locus_tag="KPK24_003525"
     CDS             5638..>6064
                     /locus_tag="KPK24_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2301159.1"
                     /translation="MIAVKKAYKFRIYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQ"
BASE COUNT         2079 a          891 c          778 g         2316 t
ORIGIN      
        1 cttctattaa acatttctta attatttatt tttcaattag ccacttctat taagcatttc
       61 ttaattactt atttttcaat tagccacttc tattaagcat ttctcaatta cttatttttc
      121 aattagccac ttctattaag catttcttaa ttacttattt ttcaattagc cacttctatt
      181 aagcatttct taattactta tttttcaatt agccacttct attaaacatt tcttaattat
      241 ttatttttca gttggctttt tctattaaat gtttcttaat tacttatctt tcagttagct
      301 ctttctatta aacatttctc aattacttat ttttcaatta gctactttca ttaaatattt
      361 cttaattatt tatctttcag ttaactattt ccattagata tttctcaatt acctattttt
      421 atcatttatt ttatagagta ttgtgcacta tatatttata catatcataa atactacaac
      481 ttgttatctc ttattttagc tgttttatct actcctctat tttttcaact atctcaaagt
      541 caatttcttt aaaatctaca ttaacacttt caacctttat cttaacaagg tcacctatct
      601 tatatgactt cttagttctt tctcctaaaa cactataaga actctcatca taaatatagt
      661 aatcatcatt catatttgca agtcttatca acccttctat ggtattatct aactcaacaa
      721 atattccaaa tgaagttacg ctagatacaa caccctcaaa ttcttgacca actttatcct
      781 tcatataaag agccttatag aaatcatgta catctctttc agctaactct gcctttcttt
      841 ctttctcgga tgattgaaca gatgcataat ccactatagt agtaagctgt tcttgtcttt
      901 ttccacttat tttattattt aaagattctt ttattattct atgaatttga aggtcaggat
      961 atcttcttat tggtgaagta aagtgacagt agaatttagc tgctaatcca aaatgtccac
     1021 tacattctgg tgagtactta gcttgtttta aagaacgaag cattatagta ctgatagctc
     1081 cttcttcttt tttgcccttt attttctcta ttataccttg taatgctttt ggatgtactt
     1141 cctctttatc accttttatt atatatccaa atgtagatat aaacttactc aattcttcca
     1201 ttttttccaa agatggtgtc tcatgtattc tatacacaaa cggtattccc atccagtaaa
     1261 aatgttcggc aattgtctca ttacttataa gcataaattc ttctattatc ttattagata
     1321 ttcttctttc atatggcttt atatctacaa cttcgccttt tccatttaga attatctttg
     1381 cctctgggaa attgaagtct atagctcctc ttttttccct tcttgacata agaatcctag
     1441 ctaaaatttc agcattttta aaatcttcaa caacatgttc aaaagtcttt ttaagttttt
     1501 catcatcctt ttctaatata tcagatactt ctgtataagt cattctagct tttgaattta
     1561 ttacagattc acatatttca tgtttaacca catttccttt atggtctatt tccataaata
     1621 tagaaagagt tagcttatct tcaaatggat ttaagctaca tactccattt gaaagagttt
     1681 ttggcaacat tggaattact ttatctacta aataaactga tgtagctctt tttaaggctt
     1741 ccttgtcaag tttatttttc tctcttacat aatgtgtaac atcagctatg tgaactccta
     1801 gtttgaagtt tccattaggt aatacttcta ttgatatggc atcatctaag tcttttgcgt
     1861 catctccatc tatagtaaat atgtttaaat ctcttaaatc tcttcttctt tttatctctt
     1921 cttgaggaat ctcaacagct acagcttctg cttcttgcaa tactttcttt ggaaattctt
     1981 ctggaagacc atgttctctt attatagaat ctatttctac gcctctttca cctttttgac
     2041 ctagtatctc tataattttg ccttctggtt ttctatcctc ttgtggccat actgtaattt
     2101 cacatactac cttatcatta tctactgctc cactaaaatg ttttttaggt atatatatat
     2161 ctttagtaaa tttcttgttg tctggtgtta caaatccaaa agttttactt gattggaagg
     2221 tacctactat tctagttatt tctctcttta ttacttttac tattcttcct tctgctctct
     2281 tttcttcagt tgcaggtact actatttctg ccataactct atcattatgc atagcaccat
     2341 ttatattgtc acttgagata aataaatcct gtgtatattc aacatcagat tctacaaatc
     2401 caaaaccctt tttgtgtgat acaaattttc caacaaagta tcccatttga ttgggagata
     2461 ctatttttcc ttttttagta aagcagatat aaccatcttc ttctaattca tttaaaaaat
     2521 tataaaacat tggcatttct gatgaatgta tatcaaatat ttctgctaat tcttcttttt
     2581 ttagaggatt atatgcactt tcatttatta atcctagcaa actttcttta agtcctggta
     2641 tcatttttcc ctcctgtttt acttttcatt atattattat ttatattgtc ttttatattt
     2701 cttcagacaa aacatttttt acttatcaca taattttcta tattttctta ttattataag
     2761 ttaaaattac ttatttatat ttttgattta atgtaatctt tgtcatacta acttcaaaca
     2821 aattatgatg ttttataaat tatttgtaaa tatcattata attacttaag ttattcttta
     2881 tcataattgc ttaaattact ctttatcacc taattataca ccatcaaata ggattggtaa
     2941 aatattcatc tcatataagt attactatat aataatttat tctattagta tactcaaatc
     3001 atgtaaaatt tatataactc aattaatttt ttaaatacaa ctttataata taaaacttat
     3061 aataaaagta aatttatatg ttaaatgagg ttgatttttt attatttttt atttatgcta
     3121 gtactaaaga cttgcaaacg ttttttgaca agtctttatt ttgtctaaat taaacaaact
     3181 tttttacaaa taaatacata aaatttaaac taatctattt attggaggag ataaaattgg
     3241 aagaagtaat tgcaaaaatt attactatag aaaatggatt caaagaaatt gaaaacattg
     3301 ctaaaaaaat tgtaaaaaat aatgactcaa aaaagtgtta ttatctatca atctacttat
     3361 ataggtcaga atattatcaa gtaagagaat tggctgtgtt tatacttggt tttatatcag
     3421 taactatttc tgaagctttg ttatttttaa aagataatgt gagtaaagat gaaaactgga
     3481 gagtacaaga aatactagct aaatcatttg attattattg cagtgaagta ggatatgaaa
     3541 aggcacttcc tgttataaaa gaatggctta attctgatag tccaaacata aaaagggctg
     3601 tgacagaagg tcttcgaatt tggactaata gagactattt taaatctaat cctgacgttg
     3661 ctatatcact actttctagt ttaaaagaag atgatagcga atacttgaga aagtctgtag
     3721 gtaatgctct tagagatata agtaaaaaat atccagattt agtaaaaaaa gaattagatt
     3781 catgggatat atcaaataaa aaagttgagt ttgtatataa gcttgcttca aaatgtatct
     3841 tggcaaagta aaatggaggt gaacttattt gaaacttcgt gatgagtata cttgcccatt
     3901 agaactaaca catgatatga taaaaggcaa atggaaatct attatattat ggagattacg
     3961 cataggacca acttccttat caaaacttga aaaggaaatc aatggtatta atcaaaagat
     4021 gttacttgaa caacttaaag aattaattga atttggtctt gtagataaaa agacatatga
     4081 aggttaccca cttaaagtcg aatactcact tacacctatt cgagggaaac aaatattgga
     4141 tgctttggct ataatgcaag atataggaat tagctttatg ttggataatg gtatggaaga
     4201 tattcttaag gaaaaaggtg ttttaaaata aatagatacc cacaaaaaag taagtaattt
     4261 accattattt tcaactggta tataattaaa ttatagatat attctattaa tttattataa
     4321 taggaggtaa attattatga aagtaactag tacaggtata gtaaatggtg ttatagaaga
     4381 taaatatggt aaaagaggaa ctcagttcaa tgaaggtggt atgcctacat attcactacc
     4441 tctaaagttt gaagatgctc caaagaatac agtatccttt gctgtatttt tagaagataa
     4501 agactctgtt cctgtatgtg ggtttgcatg gatacattgg ttagctgtaa atataacaaa
     4561 agatgagtta aatgaaaatg aaagtgtaac agctactgat tttgtacaag ggactactag
     4621 ttggcatggt aaactaggag gtcttgacag attagaggcc agtgtatatg gtggaatggc
     4681 tcctccagac agacctcatg tctatgaaat acatgtattt gcacttgata ctatccttga
     4741 ccttgaaaaa ggattctata tgaatgaatt tttcaaagct attgaagggc atgttttaga
     4801 tacatttaca ctaaaaggaa gttattctaa ttaattaggt tgactatagc taatcattaa
     4861 ttaaatgata catttaattg atgattagct taattgttat tagaatattt tctgcacttt
     4921 atcttaatat atattttagt tgactggtat tttaatttct attagattat tatcaattta
     4981 tataaattga ttaaaaaata gctacgcaca ctcgtgactt tagtcatgag ttagtagcta
     5041 aaatagtcag catataggga aacttatatg tagagatagg atagaaccta tccaatacta
     5101 cttgaattgc taaaaacccc taaagctaac caaactaaaa cataaggatg aaataaacct
     5161 aagtgtgaaa gttacgaaag tagaaaaaat tggttagatg gtataaggtt aaaccctaag
     5221 tactttaaaa tgggcaatca gcaggtaaac tccaaatagg agaaacttca acgactattc
     5281 ctcttgaggg aagtacacta caagttatcg gtagtggaag tgggtagacc ttagcagata
     5341 atgccgaagg ataagatata gtctgtgctt atgtgaaagt gtaagaagtt cataagagaa
     5401 ctgcataggt agtagcgaac ttatgtgaac gacaacctca aaaatgataa aagttaagac
     5461 ttacggttct tatatatgtt atttaaaatt aaatataaaa ccttctaaat aattgtttta
     5521 aataattaca tttgttaaca ttattatagg tgattaataa attgttcacc acagcattat
     5581 ataatatatt aaatattaaa atcattaaaa agtgtatcaa aaaacaatta aagtgaggtg
     5641 atagcagtga aaaaagctta taagtttaga atatatccta ataaaaaaca gcaagagtta
     5701 attaacaaaa cttttggatg ttgtaggttt gtatataata aatatcttgc taaaagaata
     5761 gatgtttata aaaacgataa agaaactttt acttataaac agtgtagttc tgatttaacc
     5821 aatttaaaaa aagaattaaa ttggcttaaa gaacctgata aattttcact acaaaacgcc
     5881 ttaaaagatt tagataatgc atataaaaaa ttctttaaag aaaaggcagg atttcctaaa
     5941 tttaaatcaa agaaaattaa tagattttca tataaaacta actttacaaa tggaaacatt
     6001 atgtattgtg gtcaacatat aaaactacct aaacttggta tggttaaggt aagggataaa
     6061 caag
//
LOCUS       DAEMGG010000180         6064 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEMGG010000180 DAEMGG010000000
VERSION     DAEMGG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.181"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            144..1508
                     /locus_tag="KPK24_003526"
     CDS             144..1508
                     /locus_tag="KPK24_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG2301160.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLVAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1647..2813
                     /locus_tag="KPK24_003527"
     CDS             1647..2813
                     /locus_tag="KPK24_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2301161.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2930..3712
                     /locus_tag="KPK24_003528"
     CDS             2930..3712
                     /locus_tag="KPK24_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG2301162.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3831..4484)
                     /locus_tag="KPK24_003529"
     CDS             complement(3831..4484)
                     /locus_tag="KPK24_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2301163.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4508..5872)
                     /locus_tag="KPK24_003530"
     CDS             complement(4508..5872)
                     /locus_tag="KPK24_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG2301164.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2283 a          755 c          922 g         2104 t
ORIGIN      
        1 tacttaataa ggggcttttt ttattggaag atttatgtta tattgagatg aatttattat
       61 gatataaaaa ataatgttag gataaattta aatctttttc taaaaatgta ttaatataat
      121 tcttaaagaa aggattttta ataatggata taaaagagtt agttaaaatg caaagaaaat
      181 attataatac aggtaaaaca agagatatat cgtttcgtat agaacagttg aagaaactta
      241 agttagttgt atcacaaaat gaagaaaaaa tacttttagc actaaagaaa gatttaaata
      301 aatcggattt tgaaggtttt atgacagaag taggcatgtt ttatagtgaa ttaaactttg
      361 ctataaagaa tataagaaaa tggagcaaaa taaaaagagt taaatcaagt atggttaatt
      421 ttccatctat atccaaagtt gttccacaac cttatggtgt tactctcata atgtcacctt
      481 ggaattatcc atttcaatta gcacttatac cattagtttg gtcattagta gcagggaatt
      541 gtgttatatt aaaaccttct gaatattctg tgtcaacatc tagtgtagtt aaagacatag
      601 tggaagatac tttttcaaaa gaatatgttg cagttgttca aggaagtcaa gaagaaagtg
      661 aaaagttact attggaaaga tttgattata tattttttac aggaagtact aatgttggta
      721 aaataatcat gaaatcagca tcagagcatt taactcctat tactttagaa cttggaggaa
      781 aaagtccttg tatcatttta aaagatgcta atatagacct tactgccaaa agacttactt
      841 ggggaaaact aataaatgca ggacaaacct gcgtggctcc agattatgtt ttagtgcatg
      901 aagatagaaa aaatgagtta atagagaaaa taaaatatta tactaataag tattttggag
      961 ataatccatg taataatgag caatttccta aaatcataaa tcagagacat tttaatcgga
     1021 tattatctct tattgataaa gataagatag tctatggtgg aaattataat aaggagacat
     1081 taaagataga gccaactata gtagacaatg ttaattggga tgataatata atgaaggaag
     1141 aaatatttgg gcctatattt cccattttaa catataaaga tttagatgaa gtaatacaaa
     1201 aaattataca aatgccaaat ccattggcac tttatatttt tacaaaaaat aagtacctag
     1261 aaaataaact tttagaaatg attccagcag gcggatgttg tatcaatgat acagttactc
     1321 atatagctac gaattactta ccttttggtg gtattggtga aagtggtatg ggctcatatc
     1381 atggaaaagc tggattcgat acatttactc actataagag tgtgttaaaa aaattaaatt
     1441 tggatgtccc tattagatat gctccatacg ataataaatt aattaaggtt cttaaaaaga
     1501 taatgtaggt tatattgtag aataataaat ataaataaaa ttgagtattt gtattctgaa
     1561 tatattttat ttttaatttg gtatatttgt tataaaagtg gtgattttgt gataaaattt
     1621 aattatatag aaataaggag gaaaaaatga aaattaattg gaatacaaaa tacacaacaa
     1681 tagcaattta tacatttata atagctgcta gtagtattat attttatcta gtatcatcac
     1741 aaattgatgt attttctaat aatttagatg ctatatttac aacattacaa ccttttataa
     1801 taggatttgc tattgcatat cttcttaatt ttattttaaa gttttatgaa gacagaattt
     1861 ttataaaaag tgagaaatta aaaaaactaa aacaaagttc aaaaagaggc ttgggtcttt
     1921 tattaacata tgcaacagct acattgatac tttacttatt tatgcatttc gtattgccac
     1981 aagtaattga gagtattgtt ggactagcaa atgatatacc tatgtatgta aacaatgcaa
     2041 ctaagcttat tgataaatta atgactgatt taaatcttga tgagcaatac tttaatttgg
     2101 cagttgataa gtggaatgag tttgttacat atattataaa gtttgtaaca gatttaatcc
     2161 caatattagg aagtatgtta aagaatgtag cctctagtat atggaatatt gtactaggat
     2221 taattgtttc agtatactta ttaattgata aagaaaaatt ctatggatta agcaaaaaaa
     2281 taacttatgc agtatttaca gaaaaacaag ctgccagaat tttagaatta actcatagaa
     2341 gtaactatac atttggaaga tttttaggtg gcaaaatatt agattcattc attataggta
     2401 ttctaacatt tgtaatactt acgttagtta aaatgccata tacattatta atatcagtta
     2461 taattggtat tacaaatatt atccctttct ttggaccttt atttggagct ataccatcta
     2521 cgctaatagt gatgtttgta tctcctataa aagctttctg gttattgttg attatattaa
     2581 taatacaaca gatagatgga aatattatag gacctaaaat acttggagat tcaattggta
     2641 tatctgcatt ttggattttg ttctccttat tggttgcagg aaaattatta ggttttattg
     2701 gaatggttat aggagttcca atgtttgcag taatttattc tataattaag gatattgttg
     2761 aaagtaaatt ggataaaaag ggattaccga cagatacttc cgattatatg tagctaaaaa
     2821 atattatgta taattaaaaa tatataaatt aagcaatatt taaagcactg agaaagttaa
     2881 actcttggtg ctttttagat ataaaattat tatagaagag gtgttttttg tggcatttat
     2941 agaatgtaaa tttaaatcta aatatactgg aggagaaact gatataattg taattttgcc
     3001 tgtagcaaat ggaaaagaac tttttagtgg taaagagata tatgattttg aaggtaaatt
     3061 taaaacatta tacttatttc atggtttaga agatgaccaa agctcatgga ttagatatac
     3121 taatgtagag cattatgcta aaaataaaaa tatagcccta gtaataccaa gagtagaaac
     3181 tagtttttat accgatatgg cgtatggaca ccaatatttt acttttgtaa gcgaggaatt
     3241 acctaaattt gttagagcta tatttccttt atcagataaa agggaagata actttgtagc
     3301 tggtatgtct atggggggat atggagcttt aaaattagcg ctttcaaaac caaaagagtt
     3361 ttcggcagtt gcaagttttt ctggttcgcc tgatatgatt catgagttag aaaatcaaca
     3421 aatagaaacc aatgcagaca ttctatttca cgattttggt agaccagaag acgctagaaa
     3481 tagtgagaat gatttacttt acatacttaa gaaccttaaa gaaagaaatg aagatattcc
     3541 taaaatttat caattttgtg gtgatagaga ttttttatat aaaaataatc agatgtttaa
     3601 aaaatatgca gattctctgg gtgtagatgt tgaatatgaa gagtgtgtag gggggcatga
     3661 atggcgtctt tggaatttat ggataaagaa atttatagat ataattggat agtacactta
     3721 tggtattaac aggtatacta taaaatatct ttaaatatga taaagactga aatcctaaaa
     3781 tcaaattatg attttaaaat ttcagtctct aagaatttaa taaaatttta ttaagttctt
     3841 acacgagtta ttattggagt aaaattgtca actatatcta tatagtattt actcaattcc
     3901 tctaaaatct cttcgcataa atccgtacta tgaccaagat taataagaaa ctgttttgta
     3961 aaatgcttta ttacagcttg agaagatata tcgtacttgt gtgaagtata aactctaatt
     4021 atttcaggtt tcatagcaag tataccaata caagaaagta ttaattcatc atgttctata
     4081 gattctttat cattaacata tcttttcatg accatttcgg ttacacttct ttgatagctt
     4141 aaaatagctt cttcataagc tatatccaaa taaaaatttc tatatgcttc gttttttaga
     4201 aggaaacaaa ggtcagcatt catagcaaga aagaacatca tcatagtacc ttcttcatgg
     4261 tagttataga ttaaagactg catttcattt cttatatctt catatattat agctcctatt
     4321 tcgcttttac ttgagaagta atagcttatt agacctaaat ttatattagc tctttctgca
     4381 atttgcctag ttgttgtctt atgataacct tgctcaaaaa ataattctct agcagcactt
     4441 ataatactag cttttgtttc tttgcccttt tctgattttg acatatttac tgtgtatacc
     4501 tctcttttca atcttttttt gaaaatttgc taactataaa tattgtaata gaaccaccta
     4561 ttataggaac caaaattctg tcaaatacag taagaatacc aatcatattt aataatgcca
     4621 atataacact tactataaca gcatagatag ctcctttttt tgtagcacct ttccagcaaa
     4681 gaccaattac aaatggtgca aatattacac ctgtacataa tggagctggc caaaacaata
     4741 attctaataa actatcaaat ttaagtgcta tgattgcacc tattatagct gtcaaagctg
     4801 atgcaatttg agaatacttt agagtatttt ttaaatcatt aaattctata ttaggatgta
     4861 ataatttgtt ataaatatct cttgatataa tagcactcat tgaggtcatt aatgaatcgg
     4921 cagtagacat tatggcagcc aatatagcag ctaagaataa tacagatatt aaaggtggca
     4981 aagtagtcga tatcatttga ggtaaaacct gaacagatga tatatttgga aataaactac
     5041 caccaatagc tccaattatt cctataggga aagcaagaaa taatagtata aatcctgcaa
     5101 ttacactact tttaactgca tttttagcat tatcagcaca ttgatatctt aaaaaacttt
     5161 cataagaaaa tattgagcct aaaactaatg gtaaaactgt atacataaaa tcactaatcg
     5221 atttatttcc tggaaagaat ggttctttta ccattgattg taacataagt aaagggttgt
     5281 cgccaactaa ttttaagctt atataagcta ctattggcaa aaacactata caaatagcaa
     5341 gttgaataaa tgctgatact gcaactcccc acattcctga tgtataagta tatataatga
     5401 taactataac tcctataatt acagctatat cataaggaat accaaatgca gtagatacag
     5461 aaccaaaaga aatcaattga ctacccataa tagctagtat agatattggt gttacaagac
     5521 tttgataagc tcttaaaaat acactatgac catatctagt ttcaatataa tcagcaggtg
     5581 ttatatcaca agataagctt cttaattttc ctgcgaaaaa tgcatttata aagcaagcta
     5641 aagctgcacc tatagtagaa tacattccaa cccaaagtcc tctttcagct ccccattgaa
     5701 ctccaccaac taagcaccct cctccaaaag atgtagcagc aatggttgca gctagtatag
     5761 gcaaaggcag atttctacct gcgattagga aatcggatgc tgttttaact ttttttgaaa
     5821 aataaaaacc aatccatatg tatattgcac acgtaacaat taaaagtaac atagtctttt
     5881 ctcctttata aaatatttta ataaaatata caaaatatat ttaaaatata ttttatataa
     5941 aagtagtacg atatttgttt tattggtttt agattaatat agctataata atttatgata
     6001 ttagatggaa attacgtaaa aatagaaaat tgaaaattct gaaatttttg tatattgata
     6061 ttta
//
LOCUS       DAEMGG010000181         5878 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEMGG010000181 DAEMGG010000000
VERSION     DAEMGG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.182"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(291..2123)
                     /locus_tag="KPK24_003531"
     CDS             complement(291..2123)
                     /locus_tag="KPK24_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2301165.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVE
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2116..4362)
                     /locus_tag="KPK24_003532"
     CDS             complement(2116..4362)
                     /locus_tag="KPK24_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2301166.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4430..4891)
                     /locus_tag="KPK24_003533"
     CDS             complement(4430..4891)
                     /locus_tag="KPK24_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2301167.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5196..5762)
                     /locus_tag="KPK24_003534"
     CDS             complement(5196..5762)
                     /locus_tag="KPK24_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG2301168.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1883 a         1087 c          634 g         2274 t
ORIGIN      
        1 attaaattct atttataata aaatttaaag aataaaatta aatatacatt ttgataatca
       61 cctccaacat tttccttact acatattata ataaaataaa cactttgtaa agacataaaa
      121 ttatgtggct atctcaaaat atagagaatc ctctttttca ttgttaaaat gataaaaata
      181 cactttattt catacacaaa agagtgcctt atgaattaaa aagttcattt taaggcaccc
      241 tcatttattt tatcttatta attacatgtc tatgctaaga tagttttttg ttacattgct
      301 tcatcttctt caaattgaga attgtaaaga ttggcataga atccgccttt ctttagaagt
      361 tcctcatgat ttccttgttc aactatatct ccttcattca ttacaagaat catatcagca
      421 tcacgtatag ttgataatct gtgggctata acaaaacttg ttctaccttc cataagatta
      481 tccatagctt tttgaatcaa tacttctgtt ctagtatcga cagaacttgt agcttcatct
      541 aatattaaaa ttttaggatc agctaaaata gcacgagcta tagtcaataa ttgtttttga
      601 ccttgagaaa cattacttgc ttcttcattt aatttcatct tatatccatc tggtaatgtc
      661 ttaataaatc tatgaacatg tgcagattta gcagcctcta taacttcttc atctgtagca
      721 ttcaacttac catatcttat attttccata attgaaccac taaataacca tgtatcttga
      781 agaaccatac caaacatttc tctaagctca ctacgattaa aatctttaat attatgacca
      841 tcaatcagta ttgaaccgct attaacatca taaaatctca ttaacagttt aactattgta
      901 gttttacctg ctcctgtagg tccaacaata gcaacttttt gaccaggttc aacatttact
      961 gagaaatcat ttattatagt cttgtttggg ttatatccaa agttgacatt ttcaaaatca
     1021 attttacctt ctaaaccatc tattgaaact gcattttcaa caatttggac ttcttcttcc
     1081 tcttctaaaa actcaaatac tctttcagaa gctgctgctg ttgattgcaa taaattggca
     1141 acctgtgcaa tctgagtcat tggctgtgta aagtttctta catattgtat aaatgactgt
     1201 atgtcaccaa cctcaattgt tttcttaatt gcaagccaac ctcctaagat tgataccatt
     1261 acatatccca agtttccaat aaacatcata agtggttgca ttatacctga taaaaattga
     1321 gatttccacg ctgaattata caggctatca tttagtttat caaactcttc aattacttca
     1381 tcctcacgat taaatgcttt tactattgtt tggccactgt aagtttcctc aacttgacca
     1441 tttacatttc ctaaatattc ttgttgatat ctaaagtatt tttgtgattt ctttattatt
     1501 cttgaaatta caaacattga tattggtaat attaaaactg ccactaatgt cataattcca
     1561 cttatagata acatcattat caaaacacct atcatagttg ttactgatgt tataagctgt
     1621 gtcatacttt gatttaaact ttgatttaaa gtatctatat cattcgtaat tctcgataat
     1681 atttcaccat gcgtcttagt atcaaaatac ttcattggca atctatccaa ttttgctgaa
     1741 atctcttttc ttaaattata ggatactttt tgagaaattc cactcattat aaatccttgt
     1801 ataaaagaga acagtgcact tatcaaataa aggaaaagta aaaaggttaa gatttttcca
     1861 attgcattaa aatctatacc tacgtttcct ccagaaactt tactcactag cccttcaaat
     1921 atctttgtag ttgcctttcc taaaattttt ggacctacta tagaaaacga cgcactacca
     1981 atagcaaata caattactag aattattgat attttaaatt tagataaata tgcaattagt
     2041 tttcgcatag tccctttaaa gtcatttgct ttttgtcctg ctcccataga ttttcccata
     2101 ggacctcttc ccattctact cattagctag ctcctccttt gaaagttgtg ataaagctat
     2161 ttgctcataa acttcgcaat tttttagaag ctctttatgt gttcctattc caactatttc
     2221 gccctcattt aaaacaatta tctgttctgc atttaagata gtactaattc tttgtgcaac
     2281 tattagaact gtactttcag cagtttttga ttttaaagcc ttacgaagag ctgcatcagt
     2341 ttttaagtca agtgctgaaa aactatcatc aaatatataa acttctggat tttttgctat
     2401 tgctcttgca atagaaagtc tttgtttttg acctccagat acatttgttc caccttgaga
     2461 aatttcagtt ttaaaagctt ccggtttaga atctataaat tctgtagctt gagctatttc
     2521 agctgctgtt ctcatttctt catctgttgc gtcttttcta ccatatttta agttggattc
     2581 tatagtacca gaaaacaata ctcccttttg aggtacatat ccaatttttt cccttaaatc
     2641 atgttgagaa acatttttta tattaactcc accaagtaat atttctcctt cagtaacatc
     2701 aaagaaacga ggaattaaat ttataagagt tgacttacca ctaccagtac ttcctataaa
     2761 ggcagttgtt tctcctggct ttgctgtaaa agatatattt gttaatatag gttcttctgc
     2821 gtctggatac atgaacgaaa catttttaaa ttcaacaaga ccctttttat cttccttaaa
     2881 ctcttgtgcc tctgattttt ctgggtcttt tatgcttata tctgacacta atacttcatc
     2941 tatacgtgta gctgaaacgg atgctcttgg aagcataata gataccattg atatcattaa
     3001 gaatgacatt attatttgca ttgtatattg tataaatgcc atcatatctc caacttgcat
     3061 tgctccagaa tcaacactgt atgcacctct ccaaactata agaactgtga taaggttcat
     3121 tataagcatc atagcaggca tcatacatgt cataactcta tttacaaaaa gatttgtttt
     3181 agttaaattt ttatttgctt tatcaaatct ttcttcttca tgtttttcag tactaaaagc
     3241 tctgataact ggaataccag taattatttc acggcttact aagtttaact tatcaacaag
     3301 cttttgaacc agtttaaact taggcataac cactgagaat aacactacaa ttaatgataa
     3361 gatagcaact actgctactg ctataatcca ggccattgaa gtatttgtgt ttaaaacctt
     3421 tatcactcca cctatagcca ttataggtgc atagaatact attctaagca acataaccat
     3481 caacatttgt atctgttgaa tatcatttgt actacgagta attaatgaag ctgtagaaaa
     3541 ttcattcatt tcagtatttg aaaatgacat aacttttcta aatactccac ttcttaaatt
     3601 tctacctaca cttgcggcta ctcttgctgc taaaaatcct actgttattg tagctaccat
     3661 actaatcatt gctataccaa gcattttagc accagtaaat aatatataat tattttgagt
     3721 tttttctgta tcaataccta ttttctcata ttctgacctt acatatgaaa cagagctttg
     3781 tgttatcata ctctctggca tactttcaaa gcctttgctc atctctttag tcaatgtatc
     3841 taactgttcc tttggcattg ctgataacaa tttaaatata tcaacatctc cactttgagt
     3901 agcttgtggt ggaagtttac tcattaactg agctttcata gcttttacct ctttactatc
     3961 gccctcaagc cctgatacta ttaaaatagc tttaccaaaa ataacattta aatcatctat
     4021 agtgtctttg tctttagtat ttaactcata aagagattca tcttttagac ctgggtactt
     4081 atctttatac ttaacataat cctttttatt cagcaaagta taattatcta gtactttatc
     4141 cttactactt ttatccataa ataaagtaat tttatttaat tcactctctc taatcacaga
     4201 tggaacagag ttttctactc caccttgttg tatacctaca tttactatat tggatgtgta
     4261 ttctggtaaa gaaaggtcac ataccgcttg aattaccaat agacagacta ttattactat
     4321 tgataatgcc gatttcttta aatatttaat taatttaaac atacatttct tcccttctgt
     4381 aatattaatt agtctaataa cattaaattg aaaaacatta ttttaaactt taaaaatatt
     4441 tttcatcttc atgcttatga catcctgctt ttaaattatc tcgtacttgc attagaagtc
     4501 ttctaagtac tattttttct tcttcactaa atcctaaaaa aaactctctt tccaattcct
     4561 tgttaaattc ttcaacttta ttacatattt ctaaaccttt gtctgtcaaa aaaatcctag
     4621 atactctttg gtccttttca tcttgttttc tttccacaag tccagctttt tccatcctct
     4681 ttatcataac tgtagttgta gatgctttta ctttcctttt atctgctaat tctttttgac
     4741 tttgcccatc tttcatcttt aaagaaaaaa gtatttgggg ttgaccatga tataagccta
     4801 atttatcaaa ataaaaatga gttcttataa aatatattcg cattaattgc aagaaaacat
     4861 catttaattt ttcttcagaa ttgctattca tacttgccct ccttattttt tatattactt
     4921 agtcggctaa gcattatttt actatactgc atgcattttt tcaatatatt tataaaaaat
     4981 tcatataaat ttcatttatt aaatattttt atatcatata gttataattt taataaataa
     5041 tagtaagatt tttaattagt cagctactta tattttttat tgtaaagctt aaaataaacc
     5101 ttttcatata ttttcaaata aaaaagttgt cccaaaataa attttgaaac aacatcttat
     5161 tcaacagtat cttgattata taacattatg atactctact tatcttcata tccatttaca
     5221 acaacatcta acttcccagt ttcaggtgat ataattagac catgtacaat aataccatct
     5281 ggaattaaag gatgcccttt tattaaatca atactatctt ttacagattc ttctgcagaa
     5341 tcgaatccat gtaaccattg ttttgtatca attccagcac tacgaagtgt aagtattaca
     5401 tcattagata ttcctctatc taaaatttta ccaagtaact tatccgtatc aacattacac
     5461 ataccacatc catgatgccc tacaaccata acctcatcta catcaaattc gtaaattgca
     5521 actaaaacac ttctcataat acttccaaaa ggatgcatta tagtagctcc tgcatttttt
     5581 attagtttca catctccatt tttcaaattc atagccttag gcaaaagctc agttaatctt
     5641 gtatccatac aagataagac tactattttt ttatttggat gttttgatgt cacatattgt
     5701 tcatattctt tgttctctac aaatgattta ttatattcta atatctcttc aagttttctc
     5761 atatgtaata tcaccctctt taaaaatttt tttattatta tatcactatt aaagtacaat
     5821 atatacacat ataatttaat ttatatttat taaattattt tataagatta ttactcta
//
LOCUS       DAEMGG010000182         5418 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEMGG010000182 DAEMGG010000000
VERSION     DAEMGG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.183"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(127..810)
                     /locus_tag="KPK24_003535"
     CDS             complement(127..810)
                     /locus_tag="KPK24_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG2301169.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(986..1576)
                     /gene="zapA"
                     /locus_tag="KPK24_003536"
     CDS             complement(986..1576)
                     /gene="zapA"
                     /locus_tag="KPK24_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG2301170.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1601..3994)
                     /locus_tag="KPK24_003537"
     CDS             complement(1601..3994)
                     /locus_tag="KPK24_003537"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG2301171.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4011..5030)
                     /gene="pheS"
                     /locus_tag="KPK24_003538"
     CDS             complement(4011..5030)
                     /gene="pheS"
                     /locus_tag="KPK24_003538"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG2301172.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5080..5314)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1644 a         1067 c          596 g         2111 t
ORIGIN      
        1 taaaatactt ctattaactt atacattgct tactatattt taaattcttt aattaatcta
       61 tgcaatattt gctatatata aagtatatca aataacagct tcattttttt gtaaatttac
      121 agaaagttaa ttttcattaa atgaatcatc tttattgata aacataattc caaagaccac
      181 tgccaaaata aaaaaacata ttagtattga tttttccacg aaaatttttg taaaaaaagt
      241 tcctaaaata gctccagata taaagaatat aataatagca taatactgta aactcttttt
      301 tatcataaaa atattcttag tatgtatata atgaaataaa gtttctgtag cacttcttaa
      361 atttccagta cacattgtcg ttgcaaaact attcccattt acttttctaa aactctctac
      421 ttgcattgaa caaataaaag aaatagcaac atttacaaac atattaaatc ttcctctagg
      481 aataaatgaa acaataagta ttgtaatcat ttcaattata atgataacct gacgccaatg
      541 aatctttgtc ttattatttg taaaatactt tcttatcttc tcggctataa atacacctaa
      601 cataaatgcc aaaataggaa tcaaataata aaatgattcc tttacattac cttctgctat
      661 attaagccca aacaaaacta tatttccagt ttgtgcatta gcaaagactt tacctcttac
      721 cacataagta taaatatcca aaaatcctcc tgttattgcc aacaaagaac ataaaaagaa
      781 tgattcagac atctgactct tgcttgtcat atataaacct ctcgctttct aatgatagat
      841 acaaaatcaa aatcatttta gattctattt ttcattgata taaaaaaatt atttatattt
      901 aaatattgtc tatatttata catcatttat atttatacat taaaagaagc tgtctcaaaa
      961 aacaagagac agcaaatctt ttagtttatt ccacattttt tgcatcatta tttaaccctg
     1021 tacattttaa ctgtaaatca taagctttgt tttgaaactc agaagctaag ttttcagcta
     1081 tagtagctct ttcatttgct tcttcaagtt gagtacttaa ctcctcaatc ttgtttttat
     1141 aagcatctaa ctctgctcta gaaccttctg tcgtattact aagttcgaat atttcttgct
     1201 ttttattttc tataatctca attaactctt tcatcttagt ttcagcttca cgattttctg
     1261 cttctttttc tgctatcgta agctttaaag acttgatttc taattgaagc tcttcattag
     1321 atgctccaac cttcttactc aattcttcat tagctttaat caacttctca ttctcatctg
     1381 aacattcaaa taaaaggtct gatatattta atgcagttaa tatcgcagca actgataagc
     1441 tcaatttcgg gttttgacgt gttattttat ccatttcttt gtctacaaaa tcagcaatac
     1501 taatcataaa cttctctgat ttgtctccaa ccattggata ttcagcgcca tggattttaa
     1561 ccattacttt gttcatccta acctcccccg acattagttg ttaatttgat cttaagttag
     1621 catttaattt ttcgcttaat tcacttaata tcttatcatg caccttagct acatcttcat
     1681 ctgttagagt tttatcttta cttctataag ttattgaata tgcaattgac ttgtatccag
     1741 cttctatttg agaacctttg taaacatcaa ataacttgta gctttctact aatccttgac
     1801 cattttcctt tattatatct tctatttgtt ttacaaatac atcatcttta acaataagag
     1861 ctatatctct tgatgtagat ggatattttg gtaatggcac ataattcttt gttctatcag
     1921 aattatcaaa tacaaagtca atatctattt cagcgacata aactctttga cctagattgt
     1981 agttttctat aacatctgga tgcaattcac ctaatgtacc tacatattta ttattataca
     2041 ctattttagc acatctacca gggtggaagg tagtattatt atcttgaggt tcaatttcat
     2101 aacctttaaa tccgacattt acaagtatag tttctatagt tccttttagt gcaaagaagt
     2161 caacatcttt accatacata cctacacaca ttctattttc ttccttaggt aattctgaat
     2221 cttgtgggat gaatatatgt ccacactcaa atgcagatac ttcttctatt ttatgagata
     2281 cattagttga taaaacatct aacatatttg gtattaaagt agttctcatt acagaagttt
     2341 cttctcctaa tgggttaagt aatttaacaa aatttctctt tgcattacct tctggtactc
     2401 ttatcttatc tactccctta gggcttacaa atgaatatgt taatatttca tttaatccta
     2461 cagcagttga attatctttt aaagcatcca tgaatttttg ttttgaagtt ttaattcctg
     2521 ctgtcgtatt tccttctaat tgtacagaag gtatattctc aaatccataa attctagcta
     2581 tttcttccca tacatcagct tcttgttcca tatctgttct aaagcttggt acatctattt
     2641 ctaatttatc attagctacc aaattacatt taaactctaa tgattctaaa attcctacaa
     2701 actgctccat tggtacatct acacctaata gatggttaat tctttgagga tttactacca
     2761 attttttagg ttctggttta tttggatata catctacaac accttttaaa acttttcctg
     2821 ctcctaacat ttcaacaagc tgtgcagctc tatttgctgc tatctctgca agattagggt
     2881 ctaagtcttt ttcatttctt gaagatgctt ctgacctaat accaactttt ttagctgtca
     2941 ttcttatgtt ttctggtttg aaattggcac tttcaaaaag tacagtcttc gtattagaag
     3001 ttatttctga gttagcacca cccattacac cagctaaacc aagtgatttt tctccatttg
     3061 ttataactag catatctttg tctaatgttc tctcaacatc atctaatgtt acaagtttct
     3121 ctccatcttt agcatttctt actactattc ttccagtctc tacttgattt atatcaaaag
     3181 catgaagtgg ttgaccaagc tctaacatta cgaagtttgt tatatcgact atgttactta
     3241 taggtcttac tcctgcttct gtaagtcttc tttgcatcca atatggagaa ggttctattt
     3301 ttacatctgt aaccattcta gcaacatatc ttctacataa atctggatta tctatctcaa
     3361 ctttgaaatc tatctcttcg tcactttctt ttacttcgat ttcaggatat tttacttttg
     3421 ttcctatagt tgcagctgct tctctagcta tacccatcat gcatttacaa tcaggtctgt
     3481 ttgaagttaa ttcaaaatct attaaagcat cttttaatcc taaaacatct tttatatctt
     3541 ttcctaattc ataagaatct tcgtggtcta aaatatatat accacctctt ttatactcct
     3601 caatgtaatg ttcatctata cctagttcag ctgctgaaca catcatacca tctgataatt
     3661 cacctctgaa atcagtctgt ttgatttcaa ctcctccagg taacttagaa ccatttacag
     3721 ctactggaat ataatctcct tctgatatat ttgtagctcc tgtaactatt tgaacaactt
     3781 tatctccaat atctactttg gttacaacca acttatcagc atttggatgt tgttttattt
     3841 ctaaaatctt tccaaccaat atattttcta tttcttcacc ataataatct attgtttcaa
     3901 ctttagttcc tgtcattgtc attttatcag cgaactcttt tacatccatg tctatatcaa
     3961 catagtctct aagccatttt aaagatacta acatctacat accctcctaa ttaaaattga
     4021 tttaagaatc tcatatcatt ttcgaataat aatctaatat catctatttc gtatttaagc
     4081 attgcaagtc tttcaacccc aactccaaat gcaaatccac tgtaaacttc tgggtctatt
     4141 ccacaatttc taagcacatt tggatgaacc atacctgaac ctaatatttc tatccaacct
     4201 tcatatttac acattgggca acctttacca ccacatttga aacaagtaac atcaacctct
     4261 gcacttggtt ctgtaaatgg gaagttgtga ggtctaaact tagttttgat atcagaacca
     4321 aacaattttt caacgaagat atccatagtt cctttaaatt ctgccatagt aacatctttt
     4381 ccaacaacaa gcccttctat ctgatggaac attggtgagt gtgtagcatc tggcgagtct
     4441 gacctaaaac atctacctgg tgcaattact tttattggta actcttgact tctcatagtt
     4501 cttacttgaa ctggagatgt ttgagttcta agtaataccc catcattgat atagaatgta
     4561 tcgctcatat ctcttgatgg atggtcttta ggagcgttta atgcgtcaaa gttgttttca
     4621 acagtctcaa cttctggccc ttctgctata gagaatccca ttccgataaa tatatctgtt
     4681 acttcatcta taatttgagt tattggatgc ttcttcccaa cctttaaaac ttttcctggt
     4741 tgagtaacat ctatcacttc ctcttttaat tttctttctt tttcaatagc atttatttct
     4801 tcttttttag aatttatgct aagttcaatg ttttctctta cctcattggc aaccttacca
     4861 actactggtc tttcttcagc agataattta cccatttctt taagtatggc agttatctca
     4921 cctttttttc ctaagtactt aactcttaaa ctttctacac tttctatgct ttgtgcttct
     4981 tttatttcag ccaaagctgc ttcacgtaaa gcaagtaatt tttcttgcac acatatcacc
     5041 ctttcgtttt tttgtataaa aaaagcccct caatccctgt ctagggacga aaggctatag
     5101 tttcgcggta ccaccctagt agtttaacaa tttaaaaatc atcaaaccac ctctaacaaa
     5161 cttaacgcgt ttttgacgtc aaagcctact gtaaaatttc agctctgtta ctccagaggg
     5221 aacttctcat gacctcttag aaaacactcc cagcctaggt gtttatctct ttactaagtt
     5281 taatcaaaat actttcctct ttcattgcat ttacaatatt atattgttta ttttagtatt
     5341 atatcatttc cattcaaatt atgtgactat ttttaatata tatccaatac ataattaatt
     5401 taattatttt ttatataa
//
LOCUS       DAEMGG010000183         5404 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEMGG010000183 DAEMGG010000000
VERSION     DAEMGG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.184"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            89..469
                     /locus_tag="KPK24_003539"
     CDS             89..469
                     /locus_tag="KPK24_003539"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG2301173.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            484..945
                     /locus_tag="KPK24_003540"
     CDS             484..945
                     /locus_tag="KPK24_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG2301174.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            966..1556
                     /locus_tag="KPK24_003541"
     CDS             966..1556
                     /locus_tag="KPK24_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301175.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            1571..2728
                     /gene="alr"
                     /locus_tag="KPK24_003542"
     CDS             1571..2728
                     /gene="alr"
                     /locus_tag="KPK24_003542"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG2301176.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            2761..3048
                     /locus_tag="KPK24_003543"
     CDS             2761..3048
                     /locus_tag="KPK24_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG2301177.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            3017..3400
                     /locus_tag="KPK24_003544"
     CDS             3017..3400
                     /locus_tag="KPK24_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG2301178.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            3571..4398
                     /locus_tag="KPK24_003545"
     CDS             3571..4398
                     /locus_tag="KPK24_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG2301179.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            4391..5332
                     /locus_tag="KPK24_003546"
     CDS             4391..5332
                     /locus_tag="KPK24_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG2301180.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVSVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
BASE COUNT         2152 a          567 c         1083 g         1602 t
ORIGIN      
        1 actaaataat aatgtgtatt atcgatagta aatttataaa ttcattatta attattgaaa
       61 gaagtattat aaattttctg gaggaacaat gaatatattt gatattggcg tagatataat
      121 tgaaattgat agaataagaa aggctgtaga caaaaataat aggtttttgg agaagatatt
      181 tacagataga gaaatagagt attttaattc taagaatttt aaagcagaaa gtatagcagg
      241 taatttcgca gccaaggaag ctataagtaa atctataggt acaggaatta gagtgtttaa
      301 ttttaaagat atagaagttc tcagaaatga aatgggaaaa ccaatagtaa aaacttataa
      361 taatctagca aaaatgtgca tagattataa tgttttagaa ataaaagttt caatatcaca
      421 ttcaaaagat tatgctatag caaatgcgat aaccataatt aaggactaag gagtgattac
      481 attatgatgg ataaaacagc aaaagaaata atgacaacag atgtaatagt agcaaaacaa
      541 gatgatagta tagcagatgt agctaatatg ctaattgcag aaaaaatagg aggtctgcca
      601 gttgtagatt cagagaatag agttgttgga ataatttctg aaacagatat tcttaaaaaa
      661 gaaaaatata tagaagcacc tctctatata aatctattac aaggattgat tttcttagat
      721 gacttaaaaa aagttgaaaa agatataaaa caagtagctg cttataaggt tggagaactt
      781 atgtctaaag atataataaa ggttcatgaa gatgataagt ttgatgatgt agctaatgta
      841 atgattaaaa aatcaataaa cagagttcct gtagtagatg atgacaacaa acttaaaggt
      901 ataatttgta gatatgatat aataaaagct ttatacaatg aataaactga agtaggaggt
      961 gtgatgtgag aaaaaagtgg accatagtat gtattatgtt cttggcactg gttataattg
     1021 taataggatg ccaaaaacga cagtccacaa aagaagaagt atacaaagat tttcaaaagc
     1081 agatttctga tatgaattat tacagttgta aagctgaagt agaagtagtt ggaaataaaa
     1141 gtccacacaa ttatgtttta attcatactt ataaaaaaac cgataactat aaattagaag
     1201 taatttcccc aaaacattta aaagggaaaa gtatagaata tcaaggtgat aaaatattag
     1261 ttaaaaatcc aaagattagt gatgtagtgg agttacctaa tacaggaaaa aataatcaat
     1321 atctttttgt aggagacttt ataaaaaact atcttcaaaa tgaggagatg aaagtaaagt
     1381 tatcgaaagg acatttggtt ttagagactt ttattcctgg ggataataaa tattttaaca
     1441 agcaagtatt atatgtaaat gctgacacaa aaaatcctga aaaaatggaa gtgttagata
     1501 aagagggagt gccaagattt acagtaaaat acaaagattt tgaatacaga aactaagggg
     1561 gactaaagac atgcaaaaaa taacagtgcc tacatgggca gagataaatc tagataactt
     1621 aagatttaac ttaaataata ttaaaaattt attagaagaa gatattaaga tttgtggagt
     1681 aataaaagct gatgcatatg gacatggtgc agtagaagtt gcaaaattgc tagaaaaaga
     1741 aaaagtagat tacttagcag tagcaagaac tgctgaagga attgaactta gacaaaatgg
     1801 cataacactt cctattttga acttgggata tactccagac gaagcttttg aagattctat
     1861 aaaaaataaa ataactatga cagtttattc tttagaaaca gcacaaaaga taaatgaaat
     1921 tgcaaaatct ttaggagaaa aagcctgtgt tcatgttaaa atagactcag ggatgactag
     1981 aataggtttc caacctaatg aggagtcagt acaggaaata atagaattaa ataaattaga
     2041 atatatagat ttagaaggta tgtttactca ttttgctaca gctgatgaag taagtaaaga
     2101 gtacacttat aaacaagcta ataattataa atttatgtct gataaattag atgaggctgg
     2161 tgtaaaaata gctataaaac atgtatcaaa cagtgcagct attatggatt gccctgattt
     2221 aagattaaat atggtaagag caggaataat attatatggt cattatccat ctgatgatgt
     2281 atttaaagat agattagaat taagaccagc catgaaatta aaatcaaaaa tcggacatat
     2341 aaaacaagtt gaaccaggtg taggaataag ttatggacta aaatacacaa ctacaggtaa
     2401 agaaacaata gctacagttc caataggata cgcagatgga tttactagaa tccaaaaaaa
     2461 tccaaaggtt cttattaagg gagaagtgtt tgatgtagtt ggtagaatat gtatggatca
     2521 aataatggtt agaattgaca aagatataga cataaaagtt ggagatgagg ttatactatt
     2581 tggagaaggc gaagttacag ctgagcgtat agctaaagac ttaggaacta taaactatga
     2641 agtgttatgt atgatatcaa gaagagttga ccgtgtttat atggaaaata atgagcttgt
     2701 acaaataaac agttatttgc taaaataaaa ccatgatatt ataatctggg aggcgatttt
     2761 gtgtacgata agagtaaaga aaaaatagaa gttactttac ctgactctct gatttctgaa
     2821 gttgatagta tagttcagat ggagaacagt aacagagaag attttgcaaa agctgctttt
     2881 cagttctata taactcagaa aaagaaaatt catattaaag aatctatgaa aacgggttat
     2941 agagaaatgg gtcaaattaa tctgtcacta gctgaattag gtatgacagt tgaagaagta
     3001 tcttctgatg ataaaaatga agggaagatt gcccaaggtg agtactaatt gtgaaataaa
     3061 gcgtggtgat ttatattacg ctgatttaag tccagttgtt ggttcagagc agggaggcgt
     3121 tagacctgtt ctgataatac aaaatgatgt aggtaataaa tatagtccta cagttatagt
     3181 atcagctata acatctcaga ttaataaagc caaattacct actcatgtag agataagttc
     3241 caatgaatat gggcttaata aagactcagt aattctttta gagcaaatta gaactatcga
     3301 taaaaagaga ctgagggaaa aaattggttg tctagatgaa aatatgatga taaaagtaga
     3361 tgatggactt caaataagtt taggattgtt tactttttaa agagggattt gtcttttatt
     3421 agacaatccc ttttttcttt taaatatctc ttaaggttta attttaatgt actatgttaa
     3481 taaaattaaa aataacgcaa aaaaatagca taaaagtgat ataataatat ggaattgata
     3541 taaaattacg aactcaaggg aggtaattgc atgagagacc ataaaggatt aaatgagatt
     3601 gcgcgtatca taagaagaga tatagtttca atgatacata gagccaaatc tggacatcca
     3661 ggagggtcat tatcagtagt tgaaatactt actgcgttat actttgatga aatgaatgtg
     3721 gattcttcaa atcctaaaat ggaggataga gatagatttg tattatcaaa aggacatgct
     3781 gcaccagcat tatatgctac attggctgaa aaaggctatt ttgataagga agaactgaat
     3841 ggattaagga agataggaag gatgttacaa ggtcatccag acatgaaagg cacgccaggt
     3901 gtggaaatat ctactggttc tttaggtcaa ggtttttcag ttgcatgtgg tatggctatg
     3961 gcatcaaagt tggataatgc tccgtggaat gtgtatactt tgcttggtga tggtgaggtt
     4021 caagaaggaa ttgtttggga agctgctatg agtgctgcac attataaact tgataattta
     4081 attgcttttt tagataataa tggacttcaa atagatggag atatagaaag cgtaatgagt
     4141 ttaggttcaa tagttgataa atttaaagct tttggttgga atgtaattga gatagatgga
     4201 catgattttg accaaatatt tgcggctctt gatatagcta aatcaacagt taaaaaacct
     4261 acaatgatag ttgcaaaaac tataaaaggc aaaggaatat cttttatgga aaatcaagca
     4321 ggatggcatg ggacagctcc aagtgatgag gagctagaaa aagccttgct tgaattagga
     4381 ggtgctgaca atgagtaaaa tggcgacgag agaagcatat ggtaaagcat tagtaaaatt
     4441 aggtcaaata aatgacaatg ttgtagtatt agatgcagat ttatcaaagt ctacaaaaac
     4501 acatgatttt tacaagtcat ttccagatag attttttaat atgggtatag ctgaacaaaa
     4561 tttaataggc gctgcttgtg gattatcaac tgctggtaaa ataccttttg caagtacttt
     4621 tgctatgttt gctacgggaa gggcttttga gattataaga aattcagttt gttatccaaa
     4681 gctaaatgta aaaatatgtg ctactcatgc aggtcttact gttggagagg atggagcatc
     4741 tcatgaaagt gtggaagata ttgctataat gagagctatc ccaaatatga cagttcttgt
     4801 tccagctgat ggagttgaaa cagaaaaaat tatttttgaa atagctaagt ataatggacc
     4861 tgtttatgtt agattaggga gaagttcagt atcagtttta tttgatgaag attataaatt
     4921 tgaaatagga aaaggtacag tcttaagaga aggaaaggat gtatcaatta tagcttgtgg
     4981 aattatggta aatgaagctc tattagcaca agaaaagttg caagaagaag gtataagtgc
     5041 tagagttata aacatgtcct cgataaaacc tatagacaaa gatttaatat tagaatcagc
     5101 aaaagaaacg aatgctattg taactgtaga agaacatagc ataattgggg gattaggttc
     5161 tgctgtaagt gaggtagtag gagagtcttg tccaaccatt gtaaagaaag ttggaattaa
     5221 agatacattt ggagaatctg gtactccaaa tgaattgtta aaaaaatatg aattaacttg
     5281 tgatgacata ataaagactg ttaaagaagc tattattgca aaaagaatgt aagagatata
     5341 aattattcat gtttgttaat aatataatat atttaaaaga gaggtataaa gtgttatatt
     5401 aatt
//
LOCUS       DAEMGG010000184         5394 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEMGG010000184 DAEMGG010000000
VERSION     DAEMGG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.185"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            342..1841
                     /locus_tag="KPK24_003547"
     CDS             342..1841
                     /locus_tag="KPK24_003547"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG2301181.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIEDEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            1978..2295
                     /locus_tag="KPK24_003548"
     CDS             1978..2295
                     /locus_tag="KPK24_003548"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2301182.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAFDDETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKLSTLLD
                     KSK"
     gene            2253..3149
                     /locus_tag="KPK24_003549"
     CDS             2253..3149
                     /locus_tag="KPK24_003549"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG2301183.1"
                     /translation="MLKKVKYPIGQVKVSDKFKTIKLLSRKYSISSMCKLISVSRSGY
                     YKWLSYSKKSSDRGIKDRIIKDYIIEIHKKYRGTYGRKRICTYLNKILDTPINHKRVY
                     RLMKELGIKSIIRKKVYRRKFKSYEIYDNILNREFRANQPLEKICMDITYIPIGKKFL
                     YMNVAKDLFNGEIVAYEISTKMDTKLVNNTVNQLINMNLAKDCILHTDQGSQYTSRSY
                     SKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPKEMISLINDYIYF
                     YNNERIQLKNGMSPIEYRTHSE"
     gene            complement(3230..4387)
                     /locus_tag="KPK24_003550"
     CDS             complement(3230..4387)
                     /locus_tag="KPK24_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG2301184.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(4502..>5394)
                     /locus_tag="KPK24_003551"
     CDS             complement(4502..>5394)
                     /locus_tag="KPK24_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG2301185.1"
                     /translation="SVLINSFLSKFLKIIGWGLVSLFTLSALVLSTISIIIGWAVILP
                     IQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAENTSKSLNFII
                     KDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEKTDESNEVDTI
                     INKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKLTQIQKFMDYY
                     LPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDYLFEDEAIDIS
                     TDITVLENMLVQEGLTSGKF"
BASE COUNT         1845 a          767 c          778 g         2004 t
ORIGIN      
        1 aatttattaa atgtactttt ggatatacta atatagattt aatttatctg tatttttgag
       61 gtttatgcac tctctagcaa tattatcaat ataaactata caaatgtaat tatatgaata
      121 actaaagatt aatttcaaat tagctactat taaacactaa acattattta atatacaact
      181 ttaccctata aggcaatata tattaaacaa attcaattaa aatagtttaa atttttcaaa
      241 aatctcaata tttgacaaaa tactttaata aaattaaaga ataagttata attaatacta
      301 tcaattataa aaagtataaa aatataagga gtgattaaaa aatggcgttt tattttgata
      361 caccatcaca tactttcagt gaataccttc ttgtaccagg tttctcttct actgattgta
      421 tacctgctaa tgtaagttta aaaacacctg ttactaaatt taaaaagggc gaggaagcag
      481 atatttatat gaatatacct ttgacttctg ctattatgca atctgtttct gatgacaaaa
      541 tggctattgc acttgctaaa gaaggtggta tttcatttat atacggttct caaactattg
      601 aagatgaagc tgctatggta gcacgtgtta aatcccacaa agctggtttt gttgtaagtg
      661 attcaaatat taaacctgat aacacattaa aagacatact tgacttaaaa gaaaaaactg
      721 gacattctac agtagctgtt actgaagatg gaacttcaac tggtaaatta ttaggtattg
      781 ttgcaagtcg tgattatcgt ataagtcgta tggatttaga tactaaagtt agtgaattta
      841 tgactcctat gtcatctata gtttatgcta acaaagatgt tactttaaag gaagcaaaca
      901 acattatatg ggaccacaaa cttaattctc ttcctgtact tgatgacaat ggtaatctta
      961 tgtatatggt attccgtaaa gattactctt ctcataaaga aaatccactt gaacttttag
     1021 attcttctaa gagatatgta gttggagctg gtattaatac cagagacttt gctgaacgtg
     1081 ttcctgcttt agttgaagct ggtgctgatg ttttatgtat agattcttca gaaggttttt
     1141 ctgagtggca aaaaataaca cttgatttca ttcgtgaaaa atatggtgat acagttaagg
     1201 taggagctgg aaatgtagtt gaccgtgagg gattccttta cctagctgaa gctggtgctg
     1261 acttcgttaa agttggtatt ggtggtggtt ctatatgtat aactcgtgaa caaaaaggta
     1321 taggacgtgg acaagctact tctataattg aagttgctca agctagggac gaatactttg
     1381 aaaaaacagg tatttatatt ccaatttgtt ctgatggtgg tatcgtttat gaccatcata
     1441 taactttagc tttagctatg ggtgcagatt ttataatgct tggtagatac ttctctcgtt
     1501 ttgatgagag tccaactaac aaagttaata taaatggtag ttatatgaaa gagtattggg
     1561 gagaaggttc tgctcgtgct agaaactggc aaagatatga ccttggtgga gataagaaac
     1621 tttcttttga agaaggtgtt gactcttatg ttccttatgc tggttctttg aaagacaatg
     1681 taactctttc tttaagtaaa gttcgttcta ctatgtgtaa ctgtggagca cttagcattc
     1741 ctgaacttca aaaaaatgct aaacttacac ttgtatcttc tactagtata gttgaaggtg
     1801 gagctcatga tgttatgtta aaagataata gaaatttata atataaatat actttaatca
     1861 tttaaacaat gaaaaagaga cttatctcta ttttagtata atccccttat agtagacatt
     1921 taaataaaag gctattataa taggtctaaa aagcgtatac tctaaggggg tttttctatg
     1981 tctaaaaaac acaaaaaata tagcaaagaa cttaagctaa aagctgtaaa cttatatata
     2041 aaagaaggat actcatcata caaaattgct gagatgctga atataagaag caaaacacaa
     2101 gttcaaaact gggtaaaaga ttataaaaat aaaggcaaaa ctgcatttga tgatgaaact
     2161 aggggcagat ttaaaaatat tagtctagaa aatgataata gaaaatttaa atctgttgaa
     2221 gaagaattga agtacttacg catggagaat gaattcttaa aaaagttaag taccctattg
     2281 gacaagtcaa agtaagcgat aaatttaaaa caattaaact tctgtctagg aagtatagta
     2341 tttctagtat gtgtaagttg ataagtgtgt caagaagcgg ttactataaa tggctaagtt
     2401 attctaaaaa atcttccgat aggggtatca aagatagaat tattaaggat tatataattg
     2461 aaatacataa aaaatataga ggaacttatg gtagaaaaag aatttgtact tatttaaata
     2521 aaatacttga tacccctatt aatcataaga gagtatatag gttgatgaaa gaactaggta
     2581 ttaaatctat tattagaaag aaagtatata gacgtaagtt taaatcatat gagatatatg
     2641 ataatatatt aaatcgtgaa tttagagcta atcaaccatt agaaaaaatt tgtatggata
     2701 taacatacat acctatcggt aaaaaattct tatatatgaa tgtagccaaa gatttattta
     2761 atggtgaaat agtggcatat gagataagta caaaaatgga tactaaatta gttaataata
     2821 cagtaaatca actaataaat atgaatttag ctaaagattg tattttacat acagatcaag
     2881 gttcacaata tacaagtaga tcttactcta aaaggcttaa ggataatggt attatacaat
     2941 caatgtcccg aaggggcaac tgctgggata atgctcctat agaaagtttt ttcagtcatt
     3001 ttaaatctga attaatatat ttaattgata ctacagatcc aaaggaaatg attagtctaa
     3061 taaatgatta tatttatttt tataataatg aaagaataca attaaaaaac ggaatgagtc
     3121 cgattgaata tcgaactcat tccgagtaaa gatagccttt tttaagtctg tctacttgac
     3181 agggacatat ccaattttga gacagtctct ttttatctta gtataatttt tatttaactg
     3241 taaattcaag aagcttgctt ttaagttcat tttctatttt tgataattca acttctgctt
     3301 cttgtctctt tattctacca tcatgttgta tcttaactac ctcatcaata gtagatatta
     3361 gagattgatt agttttctta attgtgtcta tatctacaat tcctctttct gattcttttg
     3421 ctgtttctat tgttcccatc ttcaaagttt ctgcattctt taataaaagc tcatttgtca
     3481 tattactaac ttcacgctgt gctttcatag cttgatttaa gtgagaaatt ccaagagcaa
     3541 gcactatttg atttttccat aatggaatgg tatttactat agttgattga atcttttctg
     3601 ccatcagatt attactattt tgaaccaatc ttatctgagg agccatttga agcgatacca
     3661 ttctagttaa ttctaagtca tgaatcttct tttcaaatct gtcacaaagt gaaaccatat
     3721 catttacaga ttgagcatct tctgttgaac cagataaacg agctttttct ctcaactttg
     3781 gcatctcata ttctttcata tcctttaatt tttgttttcc tgctataata tacatagtaa
     3841 gctcttttga gtaagctaag ttcaattcat acatcttatc aagcattgct atatctttca
     3901 atattttaac ctgatgcttt tgaagctctt tgcttacttt atccacattt acctctgcac
     3961 tatcatactt agtttttaaa tttgaaattt tattatttgc ctttttaaaa attcctaaaa
     4021 aaccactttg ttctttttca tctgctgaaa atgcttttaa atcagtaact agatttgtaa
     4081 gcatatctcc aacttctcct aaatctcttg tctttacact ctttaaagct gtttcagaaa
     4141 aacttgctac ctttttttga gcaccagctc catattgcaa aatcatattt gtatttgtaa
     4201 tatctatttt ttccgcaaat tcctttacca tcttgcgttc agattcagac agtttttctt
     4261 cttctgggtc tttttcatcc tttttcatta ataagtcaga attatctata tcattagtgt
     4321 ttgattcttc tccaaatggg tcaagtgtca aagttggcat aactggtata tcatctaaat
     4381 tattcattgt atctttctct cctcttcctt cataaagtac tgattaactt atataataca
     4441 cattttatac tttatgacta attaattgtt taaaaataac agaatatttt ttctattgaa
     4501 gttaaaattt accactcgtt aatccttctt ggactaacat attctctaaa acagtaatat
     4561 ctgtagaaat atcaatggct tcatcttcaa acagatagtc caattgattt tcaaaagcta
     4621 ctacaatcgt attcattgta ttttcaatac tttccatagt tgttgttata ttttctctat
     4681 caattccttg ttcttccaat gtatgatatg aatttaatag ttttaaagtg gttggaagat
     4741 aataatccat aaatttttgt atttgagtaa gttttgatgg gtcatgtttt acaacatcaa
     4801 atattttact tgtaacatct tctacttgta caattttatc gcccattgat tctgatttaa
     4861 tatttttatt agcttctttt atttgtgtta gataattcat tcctttattt ataattgtat
     4921 caacttcatt ggattcatca gtcttttctt cttctttgac ttcatatgct acatcttcat
     4981 atcttggtgg aacaaaatct tcatttgtta acacaatctg attatttctt ttatcaatat
     5041 aagtatttat aaagtagttt ttgtctatca tcttttgtac atcttttatt ataaaattta
     5101 atgatttaga tgtgttttct gcaataagtt ctactggaat aatatttcta ttgtttataa
     5161 ttgtacaata ttttttataa cgagaaattt ggtctcctaa tctcttacct ttaaaaatac
     5221 aaactcctcc tataagtaaa aatactgcac atacttgaat aggaagtatt acagcccatc
     5281 caattataat ggaaatagtt gaaagtacta aggctgataa tgtaaataag cttaccaaac
     5341 cccatccaat tatttttaaa aatttactta aaaatgaatt tattaataca gatt
//
LOCUS       DAEMGG010000185         5383 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEMGG010000185 DAEMGG010000000
VERSION     DAEMGG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.186"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            405..1931
                     /locus_tag="KPK24_003552"
     CDS             405..1931
                     /locus_tag="KPK24_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2301186.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1959..2516
                     /locus_tag="KPK24_003553"
     CDS             1959..2516
                     /locus_tag="KPK24_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301187.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            3038..4081
                     /locus_tag="KPK24_003554"
     CDS             3038..4081
                     /locus_tag="KPK24_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301188.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            4192..5334
                     /locus_tag="KPK24_003555"
     CDS             4192..5334
                     /locus_tag="KPK24_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2301189.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2285 a          443 c          943 g         1712 t
ORIGIN      
        1 tctatttaca aaagaatata agtaagaatt acaattaatt tatataaaat aaatttttaa
       61 gtaaatattt ttatagagag attgttatat tacatatcag aaaaatatta aactaactaa
      121 atcaataaaa aagttggtgg ataacaattt ataattgtta gcacaacatc atcttatgta
      181 ttgaaatcta aaatatcctc atttttactt agcataaata ctattaatta agaaaataat
      241 atagaaatta taaatatata taagtttagt ctggtgtata aaacaagaaa actaagtgat
      301 ttttaattta actttttatt aagcttattt tttatactat ttataataaa taaactccat
      361 gatgggaaaa ctattactat ttaatatgta aaggagtatt ttaaatgaat gaatcatatt
      421 ggtttttaaa ctcaagtcca aaagaatata acaaattagg tgaaaatata aaaacagatt
      481 gtcttatagt aggtggtgga ataactggat tgacaactgc atatttatta gcaaaagaag
      541 gtaaaaaagt agtgcttgtt gaggctgata aaataggata tggaacttct ggaaggaata
      601 ctggtaaggt aacttgtcag catgatatat tttactctaa gatagagaaa aaatatggat
      661 tagataaagc aaaatcatat tataatgcaa ataatgaagc tttaaattta gttgaacaaa
      721 taatagaaga aaacaatata aaatgtgatt ttaaaaggga aacttctttt gtatttacag
      781 aaaaagagga tactataaag gatattaaag atgaatatcg tacatgtaaa aaaataggaa
      841 taaattgtga gtatcatgaa actatagaaa atattccatt agatataaag ggggctatta
      901 gttttactaa tcaaggtcaa tttaacccaa agaagtatat tgatggattg gcaaaagcag
      961 cagtaaattt agggctaaaa atatatgaaa atacaccagt agtagacctt gaaaaaggaa
     1021 aaatatgtag ggttaaaaca agagaggata atataataga agctgagaat gttataattt
     1081 catctcatag tccatggtat gatggattga atttttattt tgcaaaagaa tatgcagagc
     1141 gtgcttattt aatggttgca gttttggaaa ataaattagc agatggtatg tttataagta
     1201 tagatgaccc atcaataact tttagacaat ataatgatgg aagtgaaaat ttactgattt
     1261 ttggaggagg agaccataaa gttggtcagg gaggaactga gaaagaaata tttgatgatt
     1321 tagagcatta tggtaaagaa gtctttaagg taaaagattt taaggggaaa tggtctgctc
     1381 aagacaatat gagttttgac aatgttcctt atattggtta tatcaacaaa agagaagaca
     1441 atatttatgt agctacaggg ttctctaaat ggggaattac taatggaact gctgctggaa
     1501 taataataaa agatttgata ataaataata attctgatta taaagatact tttaatccat
     1561 ccagattagg tagctacttt tcaaaagatt ttataaagga aaatgcaaac gtagcaataa
     1621 attatgtaag tggaaaatta aaaataggta gtggagatat gcctaaaaat aatggagaag
     1681 gaaagattgt taatattgat ggaaaaagat atggtgtata taaggatgat aatggagatt
     1741 tttatattgt agacactaca tgtactcatt taggatgtga acttaatttc aacagtgaag
     1801 aaaaaacatg ggactgtcca tgtcatggtt ctagatttga ctataaggga aatatacttg
     1861 aaggaccagc tcttaagcca ttaaaacttt atggacatgg tgataatgat gttaatccta
     1921 agttattata aaattatttt taggaaggta aagtgtatat gaataaactt ttaaagccaa
     1981 taactatact attgtatata gtttatattt tatctgccgt attttttgta gcaaacaaag
     2041 aatatggaag tctaggtctg gtagcaatta gtttagttgg tactttcatt ttgagtttta
     2101 taaataagca aacggaaaga ttattgagta aagaattata tataatgctt gctttattta
     2161 taatgttttc atcattgtta ggtacttgtt ttaattttta tgatataaat tattatgatg
     2221 attttttgca tttatggtca ggtataatat cttgtattgt ggctttttct ataattaaat
     2281 acttttatac acaaagagag ataagtaaaa tgagtaaagt gtttttagtt atatttttat
     2341 ttatgtttag tatgggagta gcgagtttat gggaaatagg tgaattttta atggatactt
     2401 ttgttgggac tactacacag gccggagggt tagaagatac tatgattgat atggtagatg
     2461 ctttaatagg cacaattata actattcctt ttatagtaaa aaaatctaat atatagtttt
     2521 aaatttttta tattaattat aatggattta attaatcatg ttgagtttca aggtctatat
     2581 gattaattaa atccattttt gttatttata attattattg aatatatttt tacatataag
     2641 atataaggtg atagatagaa attgttggat tatgtaaata attaatatat aataccatta
     2701 tgggcaataa atacaaatca aatgataata tagtctattt atgtaaatgt tatattgtat
     2761 actaagtata atagtaagtt ttttgatagt ggtgtagaaa gtaaaaatct actagcctaa
     2821 attgatgtat ggattagttt aggaacagta attgcatatc ttaagtttga tgtcatgttg
     2881 ggctactaga aataggtcat ctttttcact tgaatatctt ttctttaaac atggagagta
     2941 tcatatcaaa aacatatgaa aattaagtga tttataagaa cagtaatatt ttatatgata
     3001 gtatagaata gtaataaagt attaggagga acattgagtg ttaaaaaagt ggtttggtat
     3061 tgtgaaaaaa agacaaaaaa gtgagtcagc aaaagaagaa ggtgaagtaa tattaaaaaa
     3121 cgaaaaaata ttatctgaag aaaagttgat agatgaagaa ggagttgtag ttaatattga
     3181 taatgaaata ttaactaaag tagaagtagt aaatgatgat aatgaaatag aagaaaaaat
     3241 aatagaagaa gattggttaa ttagtgaaaa taccataaaa ttagatgata aagaagcaat
     3301 tattaatgat aaaaacatag aattatgtaa aaaagaagtt caagttgaaa gtgaaaaaat
     3361 aggtttaaat aagtttgaag gacttgacca aaatgaaaat tataatctag aaaaaaatgt
     3421 tattgaagaa aaaaaagtaa gtgaatgttt gacagaagaa gatttagagt acataaaaga
     3481 aattaaaata aaaagaggaa aaagtataaa agctataaac ttgtatacta aagaagagtg
     3541 ggtttttgac actcatatac agtgtagtaa aaaactcaaa gttccattag ggtacataag
     3601 agaaaattta aaatatggat atatggatta ctttggagat gcaataaatt atttaagtga
     3661 agtattaaat atagatgaat actgcaaaag tgaatggagc tatctcgata atagcaaatc
     3721 tccatctgag atatttaata ttctaaacaa taaaatattt agtataaggc tttctaatga
     3781 aaaaagaaat gaaattttga caaatgataa aattgaagca ttaaaaatga attatagatt
     3841 tgaatgtatt gatgaagaat atgatgaata ttttaaaaaa tataagtcta taatcaaaag
     3901 aggtggaaag aaaaaagttg aattagtaaa taaaaaaggt gacattttag aaatatttaa
     3961 gtctttagaa gaatgtgcca tttatttgca aaaggaaaag aatgaagtta tacaaatgtt
     4021 aaaatatgga gatacaaaag taggaagaaa ctttataagg tatagtttga gaagtattta
     4081 atttcatttt aaattcataa gagtttcatt tctgattcac acatttaata ttattttaat
     4141 gtaaaataat aaaggtgaag tattgcacaa ttaaaattaa ggagggtatt aatgtctgaa
     4201 gaaaaaaatc caaaagaaaa tacattagat gaatctaaaa attcagataa tgtaaattct
     4261 aaaaaagatg atgtgtcaga tgaaaaaaat actgataaca aagttaaaaa aagggaaata
     4321 aagctaaaca acaaaacact taaagctatt gtagcaattg ttgcagtagg agctatatgt
     4381 ttaggtggag gctatgtttt tggcaaagaa gctggaagaa aattgcctgc tacttcaaaa
     4441 cactataata gcagtgaggt agtagctact attggggata aaaaaataac aggtgaaatg
     4501 ttaagaaaga gaatggagcc attattttat ttaaatgcaa aaactactat gactgatgac
     4561 gaaatacgtt catatgaatc aaatatgatt gaaactatga ttacatctga tatattatcc
     4621 tctgaagcag caaaagagaa aataagtata actaatgacc aactagatgc tcagtataat
     4681 aatttaatga gtagtataga atcagcaatg ggaatgacta aagaagagtt tttaaaacag
     4741 tttgatttga ctgaaaaata tataaaagat gatatgaaaa aagagctagt agcatcaaaa
     4801 tatttaaatg aaaaatcaaa agttactgat aaagaagcag agaactatta caataaaaac
     4861 aagagcaatt atttacaagt tagagcttca catattttaa taaaaactgt tgatgataaa
     4921 ggtaagcaag ttagtagtag caaaaaagct gaacttaaga aagaagctga agaaatactt
     4981 aaaaaagccc aagctgggga agattttgct actctagcta aaaaatactc tgaagatagt
     5041 agtgcagaat caggtggaga tttaggtttc tttggtaaag gtcagatggt ggaatctttt
     5101 gaaaaagcag cctttgcatt gaagaaggga gaggtatcta acaaacttgt agaaagtgat
     5161 tatggatatc atataattaa gaaaactgat gaaaaatatc agccatttga agaggtaaaa
     5221 tcagatttag taagcagttt aacatcagaa aaacaaaacc tacttataca aaatctaaaa
     5281 gaaaaatata atgttaaaat tacagataaa tatcaaatga aagctaaaga atagataaag
     5341 tcaatttatt aaaaaaaaga aaaattaact gtatggataa aag
//
LOCUS       DAEMGG010000186         5278 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEMGG010000186 DAEMGG010000000
VERSION     DAEMGG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.187"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(271..1890)
                     /locus_tag="KPK24_003556"
     CDS             complement(271..1890)
                     /locus_tag="KPK24_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG2301190.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDEHFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            complement(2199..2438)
                     /locus_tag="KPK24_003557"
     CDS             complement(2199..2438)
                     /locus_tag="KPK24_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2301191.1"
                     /translation="MNYNLIKVIFSLFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSI
                     LDVAVLIGFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            2709..2900
                     /locus_tag="KPK24_003558"
     CDS             2709..2900
                     /locus_tag="KPK24_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301192.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(2989..3801)
                     /locus_tag="KPK24_003559"
     CDS             complement(2989..3801)
                     /locus_tag="KPK24_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG2301193.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            4223..>5278
                     /locus_tag="KPK24_003560"
     CDS             4223..>5278
                     /locus_tag="KPK24_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2301194.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQ"
BASE COUNT         2038 a          653 c          684 g         1903 t
ORIGIN      
        1 tgaagaatta aaatgaagat aaataaaaca aatattggtt ataattgatt aaaaaatagt
       61 tatgtatact tgtgacttta gttatgggca gttcaagttt tatgaaagaa gtcatatata
      121 tatggaatat tctaggcaga aattgtttaa taataagaga atactataaa aatttgagta
      181 ttacataaaa aataagaaac cataaatgat ataagcatta tctatagctt cttacttctc
      241 attgattaag attatatata aaaataatca ctatattttt tgtttaagtt ttagtacttg
      301 ttttactata tttgttgagt ggtcagaaat tctttctaga ttagtaagta agtcaagata
      361 aagtacacca gcatcaattg tacaacttcc attatttagt ctaatcatat gtgaatttct
      421 aaaagttttt tctaaataat ttacttcatt ttcaacttct aaaactttat ttgctaactc
      481 aaaatcttta acatctaaaa ctattaaggc atccttaaaa ttagtaattg tcttattgta
      541 tatttcgtat atttctctta tcgcactatc agaaaaacta atgttcattt ccttaacact
      601 catggccaat tcagctatat tttctgcatg gtcagagact ctttcaatgt catttacagt
      661 attaaaaagc aaatctgttc taaagtgttc atcatctcta agaggttctt tagaaagttt
      721 taacaaatag ttcaatatat ctttctgtaa cttattgaca actttttctg actcaaaagc
      781 tttattaata tcttcattgc tcttatttaa aaatcctgca attgaatgtt ccaaagagtt
      841 tgtagcttta tgacccattc ttacaacttc ttgtacagta ttaccaagtg ctatagatgg
      901 agtgctaaac atcctttcat ctaagtactt agtattattt actatttctg attcagtttc
      961 tttaataggt ataagtctat ttgctaattt aactatgtat ttagaaaatg gtaataatat
     1021 tataacattt gtaagattaa ataaagtatg tgcatttgct atttgtctag ctgtatcagt
     1081 tgggtcaaaa tgagtaacta tggctgatat aggtttattt aaaacaagca taaatattat
     1141 tgtaccaatt acattaaaac ttaaatgcat tacagctgca cgtcgagcat ttctacttgc
     1201 accaacactt gataaaagtg aagtaacaca agtacctata ttatctccat aaagaatagg
     1261 taaagcagat gatagaggaa gtattccttg agaagctagt gctattaaca tacccataga
     1321 tgcacttgaa ctttgaacaa taccagttat ggcaaatcct gccaatatac ctaatacagg
     1381 atttttacta aaatataaaa gtgcatcagt gaatgctttg tattcagcaa gtggagctac
     1441 tgcatctttc ataaactcca ttcctgtaaa taatatacca aaacctataa gtatctcaga
     1501 taaatgcttt gttttttgat ttgatgtaaa taaatagaat aaaataccta tacctaaagc
     1561 aattggtgca attccttcaa gattaaatga aactaattga gcagtaactg tagtacctat
     1621 atttgccccc attattacac caatagcttg acttagattc attatacctg catttacaaa
     1681 tcctacaacc ataacagtag tagcacttga actttgtata atacctgtaa cgactgtacc
     1741 taccagtaca cccataacaa cattacttgt taagagttca ataatttttt ttaatttgtc
     1801 tcctgctgat ttttgtaaac cttctcccat taaactcatt ccgtaaagaa aaagtccaag
     1861 tccaccgata aggctaatta ctatattcac gatatttcct cccttaagaa attaataatt
     1921 tttttatctg aattataaac tttattaact aaaatttatt gtttccataa tatagaataa
     1981 caaattttgt caataaaaat attaatgttt tgttaagtat acgttaagag aatgttaaat
     2041 ttttaagttg ttgttaattt tagaaaattt atgatatata gcaatttaaa tacttgaact
     2101 tagattttta gtattttatg tacaaacaat ttaacaaaga attaactata attgtaaaaa
     2161 atatgtaaaa aaataacatc cttttagaat gatatttttt agttataaat atttttatat
     2221 tcaattggtg taaagttatt aaacttttta aaaattatac tatagtaatt ttgactattg
     2281 aatccaatta gtacagcaac gtctaaaata gacaagtctt tttttaatat atactttttg
     2341 cttttttcaa ctctaacttt atttaaaaaa ttagtaaaag tatatccact ttttttctta
     2401 aacaaagaaa aaataacttt tattaagttg taattcatta catatattac ttactgttat
     2461 atcttctgag taatttttgt gaatataatt tatagttttc tttaagtttt ctttacataa
     2521 aactaagagt tggtctatgt ttcattttat caaggcatat ttattaaaaa atcaatctct
     2581 atttgttaaa ataatttatg aatgatgtta gaaactgtaa tatattggat ataatataat
     2641 aaacagataa actctgcatt aagaggatat atagtttatc ttaattatag gggaagtgag
     2701 gatatattat ggaaagatta atggaactca tagaaagaaa atttttaaag gatgtagaac
     2761 ttttagaatt agaagacata gaaaatgtaa tagaatgcaa aatgacacag ccatataatg
     2821 ctcattataa tgaatattta gtaaaagtaa aaaatggaga tgaagtagaa gaatattttg
     2881 tgtatttaaa acatagataa acataaaaga ggtttatatt tatttgtatg attataaaat
     2941 aaatacaagc ctcttttttt gataaatata aaattataaa atttttattt atttaatttt
     3001 ttcaacaaca ttagtcaata ttgaatctaa atcatcacta atttctgtaa ctccattatg
     3061 agtaatatct ataatttctt tagtcatagg aagttctcct aaaagctcta agtctaaatc
     3121 atctaaaaac ttttcagtag attctccttc aaataacttg atttttttag aacaatcagg
     3181 acattggata taactcatat tttcaataac tccaaggaca tttatgttca tttttttagc
     3241 catattaaca gcttttgata caatcataga taccaaatct tgaggtactg aaaccataac
     3301 tattccactt ataggaatag attgcataac tgttaaggca acatctccag taccaggagg
     3361 catatctatt aataaatagt ctaattcttc ccaaagtaca tcagtataaa attgctttac
     3421 aacccctcca agtaaaggtc ctctccaaac aacaggttca ttttcatcat ctatcataag
     3481 gtttatagac attactttta tgttatttga gttttctaca gggtaaatat aagagccatc
     3541 agaataagct tttacatttt ttacacccat aagtctagga atacttggtc cagttatatc
     3601 tgagtctaat ataccaacct tatatcccat tttgtttagc ttattagcaa gtaaagcagt
     3661 tacagttgat tttccaactc cacccttccc actcataaca ccaattattt ttccaaattt
     3721 attgttaggg ttattttcaa tagaacaatt tgattgacta ttacaatttc cctttgatgg
     3781 acatgaatta caatctgcca tatctattac ctccaaactt taatatttat tagattattt
     3841 gtaacctaca tatactattt tagtacaatt gaaccataaa gtcattattt aaaagcaact
     3901 tagaagattt tctattgatt tattcataga ctatagcaaa aagaatacaa taaaaaaatt
     3961 ataacattct tgaaaaagtg attatttcaa tttagttata gaacttagtc atataaatat
     4021 atagaaaata ttttaatata ttatacataa cgggttattt tataagttta actataaatt
     4081 aggtttatat tttataaaaa tttgctaaaa aaaacaatta aaaattgtaa agaaaaaaga
     4141 tataattaga actgtagtaa taaatgtttt attatagata atagagtttt aagtatctta
     4201 aataatattt ggaggaatat gaatggaatt aagaccaaga agtgcaaaat tagaagaatt
     4261 tgataaagta atagaattaa taaactatgt atttagaata tcaagaaatc ataaaccaac
     4321 tatgatggaa gaatttcctc ttttactaag taaaaataat atagaaaata tgattataat
     4381 atcagaagat gataaagtgg tttctgatgt aaactatcta attcaggatg tatctattca
     4441 aggaaacaga ttgaaggttg cagctatagg tggagtatgt actcatccag attatgaaaa
     4501 gagaggctat tcatctaaga tattggataa agtagaagaa aaaatgtttt atgatggtgt
     4561 agatattgta attatatcag gaacaagaag tctatacagt agaagaaatt gtagtttggt
     4621 aaagagtttt tataaatata caataaaacc tgaagatgta aaaatagcat atgaaatagt
     4681 tgaatttgat gaaactaatt ttgaaaaaga taatgattta gataaaatga ttgaactata
     4741 taatcaaaat agtactagat ttataagaac gagagatgag tttcaaaaac tattgcatgc
     4801 agcaactatt gcctggggac caattggtta caagaaggta tttataaagg aaaataataa
     4861 tataatagga tatttgataa taagaacaat aaaaaaagaa gattctactg taggagaagt
     4921 tgctgaaata gggttaaact ctgtaaatgt tgaaaatata ttgaaatatg ttgcaaataa
     4981 atttggatta gaatctttaa attataaagt acatgtgaaa aatcttaaag accagttgaa
     5041 atgtaatggg acaaaatctt tggattatca acaaggaact atgaaaataa taaattttac
     5101 taagttgtgt gatagtttaa gaagttattt tagtcaatat gtagattttg aattactaaa
     5161 atacatggaa tttaaacaag ttgaaaataa atatattatt aaatacaaag aagaagaact
     5221 tgtaatagaa aatttggata agcttaataa actgtttttt gaaaaaaatg aggaacag
//
LOCUS       DAEMGG010000187         5252 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEMGG010000187 DAEMGG010000000
VERSION     DAEMGG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.188"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(392..1798)
                     /locus_tag="KPK24_003561"
     CDS             complement(392..1798)
                     /locus_tag="KPK24_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2301195.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1843..2550)
                     /locus_tag="KPK24_003562"
     CDS             complement(1843..2550)
                     /locus_tag="KPK24_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2301196.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2721..3635
                     /locus_tag="KPK24_003563"
     CDS             2721..3635
                     /locus_tag="KPK24_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2301197.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QKLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3635..4396
                     /locus_tag="KPK24_003564"
     CDS             3635..4396
                     /locus_tag="KPK24_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2301198.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMFMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4408..5145
                     /locus_tag="KPK24_003565"
     CDS             4408..5145
                     /locus_tag="KPK24_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2301199.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1803 a          808 c          690 g         1951 t
ORIGIN      
        1 acacagaaca gcgtataatt aaaacttttt taggatgtca ttatattttt tcttaaaatt
       61 aaaaaacact ataccataat tctattttag tagaattttt ttgtcaataa taaaattaaa
      121 tattaactaa aaaataaaaa aaatggaatt atctaaagaa tttaataacc ttacaataat
      181 tccaaaatat tatttctatg aaaattttga taccatgagt aactcagcca taagcgcata
      241 aagcacaagt tactctttac tttaataaaa cttgacaaga aaagaattta tccttattct
      301 actctaatag agttaatttg tcaataaaga ataaaattat tttcaaaata ttttcattct
      361 tcattcttat ctgttattat tttataaaat atcacttaat tagaggtaat tttagtattg
      421 tttttagtcc ctgactatca atgctcgtaa tatctaattt acctctatgt gcattaataa
      481 tgccagatac tatcaaaata cctagaccat gattttgacc tttggcatat gcctcactta
      541 gtccattttg gctctttaat aaactattaa ttctttcttg actcatacct tttccatcat
      601 cttcaacaat tatatagcaa aaatctaaat cagacttaga ggtaaccctt atattacatc
      661 cttgttggtt atgagttata ctattttgta ttaaattact aatagccctt tttaataatt
      721 ttttatctgc atctataata atatcttcat ttagtatatc aagttcaacg taaaagtcat
      781 tatccaaatt attatttata aactcagaaa taagctctct caaaaacgca gaaactctaa
      841 tcttatcaca attcaatgct tgcatattat attcaagttt agacactaaa tttaggtcat
      901 ttactaattc tctgatttta atcccttgat tgcatataat agacacttgc tcttgttctc
      961 tatttgaaag gctactgctt tcttcaagtt ctccagcata tccaattatc attgaaagtg
     1021 gagttcttat atcatgagat attcctgcaa tccagttaga cctagcttcc tctttcttcc
     1081 taagtttttc atctttcatt tgcaactctt tagatacagt attaatagat tttgaaatat
     1141 cacttagtat tcctttttct tgtaaaagta ctggttctcc ttttgacata agctttatgc
     1201 cagtaataat tggttttata gatttcacca tttttaaacc tatgaataga gctattaaaa
     1261 aagtaactat tgcattaacc aaaaacatac ctattattaa cataggaaat tgttctattt
     1321 ccttaaccgt ataagtaagg ttaaacttag tgtacttgtc ttttggataa cctgttacta
     1381 atattccatt tggatgttcc catacataca caggatagtc ctttaaaaag aaccttgaaa
     1441 actttgctac atctgtcaaa gtataatgac ttggaatttc ttttggtaaa tcaaatcccc
     1501 atattctatc tccattgtta tcaattaaca ttgcccaaac tttattttta tgtagtactt
     1561 ccttactaga atcatctaaa ataaactttc catttcttaa aatcaaatta tcttcaatca
     1621 taatcaagtt tgattttatt tctctgctat cacttgtatc taaattatca gagtatttag
     1681 atacaaatag catatatgaa aacagaagaa aacctgctat atttaataca ataaaaagta
     1741 atgaaatcaa gatacttgaa cctataaatc gcctcaatat gtactttaca ctatccaaaa
     1801 ttaaatcact cctcttgatt tcttttattc taatatctaa aattaattaa tcatcaactt
     1861 atatccaaga cctcttactg taataagatg tttaggtgta gatggagttt tttctatctt
     1921 ctctctaacc cttctaacat gaaccataag ggtattttca tatccataca tatcatctcc
     1981 ccaaatggct tgacatagtg aatcattagt aacaattctc cctttatttt catacaactt
     2041 taaaagcaac gaatgctctt tagctgttaa agaaatctct tgcccatctt tctcaacaca
     2101 tgcactattt aaattcacaa caacagaatc tcccagttta aatactggca actcctcttc
     2161 ttctataggc acatatacac gatttaatat acccacaagc cttaaagtaa gttcctttgg
     2221 taaaaatggc tttactatat aatcgtcagc ccctaagccc aaaccaatca atctatcttc
     2281 atcttcacct cttgcagaga gaaaaatcac tggtatttta gatgtctctc taatttttct
     2341 cataatagaa aaaccatctc catctggcaa tacaacatct aaaattgcac aatctggctt
     2401 tacatttcta aataccctaa cagcttcctc ataacttgat gctaaaaata ctctactaaa
     2461 cccctctttt ctcaggattt catctatcat ttttaataat tccttgtggt catcaaccac
     2521 aagtattttt ttatctttta ttgactccat aatcatcacc ctcctttagt ataccatata
     2581 cccattttca caactttgcc taaatagact tttaaggtta ttgtaaggta aatttcagct
     2641 aattgaaatg taaccaagtt attatactga tataagttaa aagaatcaat aagcacagat
     2701 taaaaaaggg gatgaaacat atggaaatga taattagaac acaaagactt tcaaaggaat
     2761 acaataaaat atttagagta aaggatatag acctaagagt tccacaagga gctgtatatg
     2821 gttttttggg tcctaatgga gctggaaaat ctaccactct taaaatgctt cttggacttg
     2881 ctaaacccac acaaggaaat attaatattt taggtaaaga gttaaatgaa aaaaatagaa
     2941 tatcaatttt aaaagatata ggttctttga tagaatctcc atcatactat ggtcatctta
     3001 cagggcttga aaacatgacc ataatgcaaa aactcttaga tttacctaaa aaaaatgtaa
     3061 atgaagcatt aaaaattgta aggttagaaa atcaaaaaaa taaaaaagtt agtcaatatt
     3121 ctttaggtat gaagcaacgt cttggtattg ctatggcaat aatgaagttt cccaaattat
     3181 taatacttga tgaacctaca aatggactcg acccagcagg gattgaggaa attagagaat
     3241 taataaaatc tctaccagaa aaatacggga tgactgttct aatctctagc catcttttaa
     3301 gtgaaattga ccaaattgcc acatcaatag gaataataaa tcatggcgaa ctggtttttc
     3361 aagatagcat aaaagaactt cataatagag gtcaaagtca gattgctatc aaaactaaga
     3421 atgttgtatc tgcacaaaaa ctattaaata ctaaaggata tatgtcactt atacaagatg
     3481 ggtacttgac tattgaaaat ttaaaagact cagatgtggc taaaataaat ggtatgcttg
     3541 tccattcaaa tatagatgta tttagaatag aagaacataa aaaaagcttg gaagatatat
     3601 tcttagaact tactggtaag gcggtgactt tataatgaaa taccttggtc tagaatttta
     3661 taaacttaaa agaaagaaga ttattctgat gaccttttta tttgtagctg ttgaaataat
     3721 gtggtgtatc ataaattcaa atagagctat tacacgtaat ccagatatgt taggtggatt
     3781 tgaatattcc tatatgttta tgagttttgc aagtttaaat ggtctattct ttcctatctt
     3841 aatatcaata attacatcac gtataagcga tatagaacat aaaggagata catggaagct
     3901 tttaaaatct tctgtgacat ctttaaatag tatttattta tctaaatttt tatgttctgc
     3961 tattttggta tctatacctg tattgatgca agtgttatct attgtgttgt ttggacattt
     4021 tagaggggtt attgagtcaa tttctgtatc tttactttta aaatacacat taggaacaat
     4081 tcttgtaagt attgctatca ttgcactaca cctatggatt gcaactgtaa ttccaaatca
     4141 aatgattgca attacatttg gcatgattgg tagttttata ggtttgacat caggtttgtt
     4201 ttatcaaggt attcgtagat tgtttatatg gtcttattac ctagagttat cacctttaag
     4261 ttatgcttat gataatgttt taggttcatc tgtctataga attgatatga atttttctat
     4321 tacgatttta gttttcttaa ttggaatgct aatttattat attggaagaa atcatttatt
     4381 aaaaaaagaa gtataggagg gatttaaatg ttaaggaagt cagttttggg agaaattcta
     4441 aagttaaaaa attcttttat atggtatgta ttattagctt tgccacttat aagtgtgttg
     4501 attggttctg gtaattttta tctaaatcaa ggcatactca aaaaagaatg gtacagctta
     4561 tggactcaag ttagtctttt ttatggggaa tttttctttc ctatcttaat cgcaatcttt
     4621 tgtgcatata tttgtaggct tgaacatatg aatcataatt ggaataatgt aatgacactg
     4681 ccagttaatt taaaaaatat ctttttatct aaacttacag tagtttcaat acttacagct
     4741 attacacaag ttttctttgt tatcttttat atagttgcag gaaatatgtt tggtttttct
     4801 ggtagtgtac ctaaagaatt gactggttgg attgctaaag gttggattat ttctatttca
     4861 atagcttcta tacaattata catgtctata aagattagaa gttttgcaac tcctattggt
     4921 attagtcttt gcctttctct atttggtatg ggattaatag tagcaaaacc tgctgttggt
     4981 atatttttcc cttattctat gttaggtagt gctatggggg ttataaagca atctgcttta
     5041 aatgttaatg acactatagt tacaatatgt gttgcattta ttactactat agtattttct
     5101 gaaattgcca atagaaattt tagaaagaat gatgttattt cttaatggta aatgctagat
     5161 attttggggt ttgaaattta tattcttttt agttgaaatc tatatccatt ttatttaaaa
     5221 atatatatct tttatttgaa ttttatatgt tt
//
LOCUS       DAEMGG010000188         5217 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEMGG010000188 DAEMGG010000000
VERSION     DAEMGG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.189"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            58..669
                     /locus_tag="KPK24_003566"
     CDS             58..669
                     /locus_tag="KPK24_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG2301200.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLIPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            967..1305
                     /locus_tag="KPK24_003567"
     CDS             967..1305
                     /locus_tag="KPK24_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG2301201.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1712..3148)
                     /gene="miaB"
                     /locus_tag="KPK24_003568"
     CDS             complement(1712..3148)
                     /gene="miaB"
                     /locus_tag="KPK24_003568"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG2301202.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(3396..4856)
                     /locus_tag="KPK24_003569"
     CDS             complement(3396..4856)
                     /locus_tag="KPK24_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG2301203.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSA
                     RLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVN
                     TVMLTNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTL
                     VNNTKSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLV
                     NDGSNKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGM
                     YKVGKDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYI
                     KIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLG
                     NAEGIVTNDTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT         1763 a          779 c          557 g         2118 t
ORIGIN      
        1 tattattgtt aaaactcaat tatataattt ttaataatat tattttagga ggtaaaaatg
       61 ataaacgata ttaaaaataa attcaaaaat tacaaaccat atataaatgg atacaagaat
      121 atgaagagag catctgtttt aattccaata gtagaaataa ataacacaca ctatatatta
      181 tttgaagttc gctcaaaaaa tttgaagcat caacctagtg aaatttcttt tccaggtggt
      241 aaaatagaat caggtgagac tccatatgaa gctgttatta gagaaacttg tgaggaactt
      301 ggaactttta gtgacaatat agaagtaata tcaagtcttg acctccttat aacaccagta
      361 aagtttatca tacatccata cttaggatat ataaaaaata ttaataattt aaatataaac
      421 aaggatgaag ttgaccatac ttttttgatt cctataaaat atcttttaga gaatcctcca
      481 aatacttata gtaatactgt aaaagttttt ccagacgaaa atctaccata tggttcaatt
      541 ccaaaccaaa aagattataa atttgaaagt ggaagttatc ctattctgtt ttataaatat
      601 aaagattatg taatttgggg aatgacagca aaaatactag aaaacttttt aaatttttta
      661 tcagaataac actcataata aatcatcaaa acttttacat aagaaattta taaaagtttc
      721 taaatttaac aattctaaaa tatcaataac tatattttgt agcacttttt tattttatta
      781 ttgtttgaat atttgatggt tttcaatgca atttcaacat aaatactatg tattagtgta
      841 tactgttgat gaattaaagt atgataatct atttaataaa gtatgaagta aatgatttta
      901 atgtgccttt atggtaaaat tactaataag taataaaatt aattaaaata tatggaggtg
      961 cttataatgt ttgctgtagg atttgttgtt ataattgcac ttgccttttt cattttaagt
     1021 gtactattat ttaatggaaa agccagtatg ctactagcag gttataactc tatgagtaag
     1081 aaagaaaaag cacgatacga taaaaaaaaa ttatgtagaa gttctagcat tgttactttt
     1141 attgtctcta taatgctatt tattatggcg tttttgggat atagaataga gagtggtcaa
     1201 atggacgaaa aaaaaatggt tcccttttct ataatattta taatagtcat actttccagt
     1261 gtatttttta atatcctcta ctctaacaaa aaatgtaaaa aataaatagt tgtaagttga
     1321 gcagttaatt ataaaagatt gactgctttt tcatgtatat tgactactct tattaaatat
     1381 tttaacattt aaaaatctat gtaaaaataa tgaatatagt acaaacttct aataatgatt
     1441 ttacctttgt ggttacagtc cactatatat aatttaatat gataacaggg aattactaat
     1501 gacaactagg taagctttta taaaatataa taaaacaaaa atagatgtac aatgaaatta
     1561 tatcttatat caaatttatt tttattaata gcaaaaactt acattagcat aacatatgac
     1621 aaattcgctt aaaataaaaa tttttaatat aatttgttct atagtacatc tattttgatt
     1681 agtttacata attttattat tttgtacaat attaattatt tactaatatc ccatttaatg
     1741 aaaaagttct aggttcagta atttttacca taactaattt accaattaaa tcatcatttc
     1801 caccttcgaa atttacgagt ttattttgtc tagttctacc agcaaactta ttttcattat
     1861 ttttacttct tccttcaact agaacttcaa ctattctatc tttatatcca tcatttattt
     1921 ctgcagaaat cctatttaca gcttctaaca ccctattaaa tctatcatgc tttatatctt
     1981 ctggtatttg attttccatt ttagcagcag gagttccctg tcttttagaa taaataaatg
     2041 taaaagctga atcatatcta actttttcaa ccacatctaa agtgtccaat aaatcttctt
     2101 ctgtttctcc tgggaaacca atcattaagt ctgtagaaaa agcaatacct ggaacttcct
     2161 ttttagcttt ttctattatt tctaaataat attcctttgt ataatgtcta ttcatttttt
     2221 tcaaaaggct actgcttcca cattgtattg gtagatgtaa aaactcacac accttatcac
     2281 aatctctaat tgcgtatatt acttcatcag atatatcttt tggatgagat gtcataaatc
     2341 gaaccctttc aattccttct atatcattca cttttcttaa aagttctgag aatgaaactg
     2401 gattttcaag tgtcttacca taagaatcta cattttgtcc aagtaaagtg atttctttcg
     2461 ttccatttgc aacaagctct tttatctcgt taattatatc ttcaggagtt ctacttcttt
     2521 ctcttcctct tgtgtatggg actatacaat atgtgcagaa attattgcat ccatacatta
     2581 tatttacaaa tgcttttaat tcaaattttc tattacttct aagaccttct attacttctc
     2641 catccacatc ccatacatcc actaacattt tgtcagaatt tattgattct gtaagtaatt
     2701 gcggaaattt atatagatta tgtgttccaa atataaggtc tacatgttta tatttttttc
     2761 ttaattcctc tacaacatgt ggttgttgca tcatacatcc acatacagct attttcatat
     2821 ctggattttt tccttttaga tgttttaatt gtcctaagtt accatacact tttagctctg
     2881 cattttctct aactgcacag gtgttatata ttattaaatc actttcttca accatcattg
     2941 acatttggta tcccatctct tctaacattg aacataattt ttctgaatca tgttcattca
     3001 tttgacaccc gaaggtttga gtaaacacga gttttctctt tccagttctg ttgaagtatt
     3061 cttcattttg tctgtgtatt tgttctatat atttatcttg ttcttgtatt ttctctatag
     3121 gtattgttac ttgttttctt ttgctcacgt ctattcctcc tagttaattt cttatattac
     3181 acataacctt aattatatca aataagaaaa atcttttaaa gatattatta tttttctagt
     3241 tgtgttttaa tagtaaatct aatgatttat atatactata tttctaaaat ataaaatcat
     3301 ataattatta taataataaa aagatgtttt aaattaaaac ttaaaacacc tttccttaaa
     3361 taaaatatat gatttactat taaatacaat tacatctatt cattttcaat taaataacaa
     3421 tcatttaata ttaaatattg atcttcctca accgttatat atcttcttcc tgaaaaagta
     3481 tcattagtaa ctattccctc tgcattacct aagctatcat ttctaacttc ataatatgca
     3541 tcagaagaat ttgacattat aatatattct ccagcaggaa tttctaatcc aattttatac
     3601 ataccatttc caaacttacc attcttggcc ttctgggctt tagcatactt tgctaatgtc
     3661 attgttgaat cctcaatttt aatatattga ccattcttta aggtgatata tctagtccca
     3721 ctaaaaatat catttgaaag tatactatca gcattacctg tactatcact tgtaacttca
     3781 taataagaag cataacttcc tgagtttgaa gttattaagt attcacctgc tgagatatct
     3841 tttccaacct tatacattcc atcttttata gtctttgtag taggtttaac attttttaca
     3901 accccagtat taatatctcc aattccattt gttatattta aacactgttg tactatattt
     3961 gaatctatat atcctataga agttatcttc ttagcatttt ttaaaatgct tttattgcta
     4021 ccatcgttaa ctaaaaccat tggaccatgt tttgataaag aagatcctac aagtgcattt
     4081 gttaattcat aagctccagc tatataaaac tctctagcat ccttataaaa cttatttatt
     4141 attgctttat tagtttcaaa acgagtactc ccccctagtc tagtagattt agtattgttt
     4201 actaaagtgg tactcattga agctgttcct cctattacat aagactttaa accactagtt
     4261 gaaaaaggta tactttgtcc attagtaaga attattggtg ctttatctct tgctgctaca
     4321 gatgcaatac ttatagcatc tgcttctcct ttatatgcat tggttagcat aacagtattg
     4381 acttttttta ttgaatttat ttcttttgca acattataac ttgtttttat tctgtcattt
     4441 cccttaattc ttacaacttt cattttctta gattttaatg aattttcgac agatgtacta
     4501 atagaatatg ttcctccaat tatataaact tttgatacat tttttaatct tgcagatgtc
     4561 tcagttggta tagtattttt ttcagttaat agtatagggg catttaaagc tcctgctagt
     4621 ccacttgcac ttaatccatc tgcaatagag ttactagtat taattaatat agcagtttta
     4681 tatgcagttt tatatgcagt tttatatgca tttttatctg ctattttacc tgctatttca
     4741 tacttatttt ttccttgtat tttttctaca gatgatattg catctactaa ttgaatattt
     4801 gcaaatacaa aagaaatact taaagtcaat gttaatattc ttttatagaa tttcataata
     4861 aaattgtccc cctcataata aatttttaat atataaatac atattcttaa attagttatg
     4921 tacttaacaa acttatgtaa aattgcaaat tatattaagt atattacaaa taaatattaa
     4981 tacaattatt catttagcta taattgtatt tacatttata ttaaaaatca actagatttg
     5041 tataaatact tataattata tttttattct taatattttt tttattatgt tattttaata
     5101 gatttatttt tcttttttaa gcatagagat gtatttttcc atatattcta tttctattat
     5161 agtttcgctt atttaattca cccaaaattt cacttaatat tagaatttta aatcttt
//
LOCUS       DAEMGG010000189         5181 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEMGG010000189 DAEMGG010000000
VERSION     DAEMGG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.190"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(779..1858)
                     /locus_tag="KPK24_003570"
     CDS             complement(779..1858)
                     /locus_tag="KPK24_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2301204.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(1942..2598)
                     /locus_tag="KPK24_003571"
     CDS             complement(1942..2598)
                     /locus_tag="KPK24_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2301205.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            2920..3126
                     /locus_tag="KPK24_003572"
     CDS             2920..3126
                     /locus_tag="KPK24_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301206.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(3279..3707)
                     /locus_tag="KPK24_003573"
     CDS             complement(3279..3707)
                     /locus_tag="KPK24_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG2301207.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(4183..5121)
                     /locus_tag="KPK24_003574"
     CDS             complement(4183..5121)
                     /locus_tag="KPK24_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG2301208.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         1576 a         1038 c          661 g         1906 t
ORIGIN      
        1 tcatgatttt attttaatat cttgcttata ttgctgaaat aattaaattc ttttatctat
       61 catattgtct cttatatcac aaacatattg tttcttatat cacaaaaatg aaagcattct
      121 tatatcttca atatataaac ctgctttttt taatcttgta ataaatatag gatactctat
      181 attctcttcc tagaaataac taataccact tatttttcta tcatatagaa agtaataaat
      241 ctttttctta ataaaattca atagtaagtg gaataaaatt ttatatgaat aagaacatta
      301 taaaaatact ttatacaata gtcaataaaa gatattagct tcaaattgtg ttgaagttgt
      361 attatttaaa gtggaaaagc aaataaaaaa gaaataatgg tataagtaga aaaatatact
      421 taaaacaagg cgaagcctct cttgtatcta aatcaagtag agaattaaat ctaaaaaaga
      481 aaataaatat aaaagatgac tcctgtacat tacaagaatc atcttatggt ttcataatca
      541 ctccatggtt tttaactgtc tgatataaag gtctcagttc actcatagca tgaccacctt
      601 ttccgattgc tgaccccgtc acttatggac aaaatgtcca taagtgatag tcctctctta
      661 aaaccctcta aatagatata tctcaagcac ctcattgaaa ccagttgctg gaaacaaact
      721 gaagcgttct ggaatagtaa aagaaattgc caccatatcc acgttactgt cctagttgtt
      781 actttgctaa cttcggagcg atttcctcta acaatacgca gtcaaattct gcctttttgc
      841 taagaaatgg cagatggtct gcatctggta taaaaaaaat ctgtttgtct ggcgccacta
      901 tttcatcaaa aaatttcttc gctggaagat aggggctatt atagtcattt tctcccatga
      961 tgtagtatac agggacctga tagtcaactc cataatttct tatgtcagct tcttccacta
     1021 aaaaccgcaa tacatcacct tgattgtcaa tcgcatttgg attgacaatg gccgtatttt
     1081 ttaactctct caatgaataa tatggggttg ccagtatact gattatattg cccaaatcca
     1141 tatttataga aagcccatac ttgctttggt atgttcttac tgtcataaga gctgccaaat
     1201 agcttggacc atattttgct ggcggatatg gtgcaatagc ttccagcgcc ttgatatctt
     1261 tttgcttccc tgcagctttc accatttcaa gtgtcttttc atagcccacg cgctcattgt
     1321 ccaccatatt aacagtctgc gcaatcccaa tgtacgcgct gtaatcctcc ggataggttt
     1381 gcacagccat agttcccagc actgttcccc agctatgacc tataaggata attttctcct
     1441 tgttgaactc ttgcttcaaa tatgctgtca cctcatggac gtcttgcaga acacgctccg
     1501 cgctcatggt ctgcaaaact gctgccggat cgttcgcaaa gtatgttttg cctacgtttc
     1561 gctgatccca gttcaccaca gtaaaatcat tttcccactc atactggtac agatgtaaaa
     1621 atggcatcat tgaattacca ggacccccat gaacccaaag gatgatgggg ttgtctgcgt
     1681 tttgtccccg gatatataac gcctgctgta tgccgccgat ctccaccatt tccatgcggt
     1741 cgattccagg agcttctatt ttatggcttg tacgtataat cgtgggggct atcagacgca
     1801 aaccggcaaa cgccagaaca aaaacagcaa ctagtatgca aagtgtgatt aatatcattt
     1861 taagtacctt ctttcatgaa tttttttagc catagatatc tcctctaaca ttaaaaaaag
     1921 tatgttgcta ttttagtttt gttactctac ctgtcccatt ggtttccttt cataaaaaat
     1981 attaattgat tctataatct catcttccag gatatcaact cctatttgtc tcataagaaa
     2041 ctgtaagaat tgaaaccgtt tacgaagtgt aggaaagtcc ccctgaaata catctgtatc
     2101 acaccaaaag ttaaagagca gcaaaattac ctctgcgcat tcatctggga actctgtgat
     2161 gatgcttcta tcctctatgc cttctcgtaa aacatttgct aatattggtg ctagtttttt
     2221 caaattatcc tgtgtaaatg ccagtatgat atgcggactt tctatcgcgc ttgtaatcat
     2281 gttactagat tcttttatca cctcatccgt taaatgccgc ctaactaaac ttctaagctt
     2341 ctcttttgct gtaagtccat gcatttcacc gagccattga tttactgtct ctattatctg
     2401 ttcaaagcgc ctttccatga ccacattaaa aatatcttct tttgaactga agtgatgaaa
     2461 aattccgccc tttgacattc ccgaagcatc aacaatattt tgcatacttg ttttgtcgta
     2521 tcctttttca gcaaacaact ttgcagaaat ggtaatgata ttttcaatag cttgctttgg
     2581 attgttcttt ctagtcatta tataactcct tcttattaaa aaccgactgt tggtttttat
     2641 tatataagct attcctgctt ctgtcaagta acattgtgtt tatggcaaaa caataaggtt
     2701 tggtggttag acaggtatta attagactaa tgcgactttc ccctagatgg aacccttggt
     2761 aaatttgcaa tctttaccgg ctttgattat atctgtaagc aaggcaggta tggccacgcc
     2821 tgccctgttt gaccctcgga tattcattcc tatccctcct ctccttagcc attacaccaa
     2881 aacgccgctc attgtctcca atggcggcgt tttaaagata tgatttctca tgctgctgaa
     2941 atatgcaaaa aatataagat gccctttatt atttttccta caatagaaaa gaaaagtatt
     3001 gaagaatggg ttaatattgg agcaaatatg ctttgttttg cacaagatac tacctttatc
     3061 acttatgtta tcaatcaatt attatctttt tataatgaga ttgtaccaat tcaaaaacaa
     3121 cattaaatta taaatctaat tttatattaa aagagactaa tattttatct atctctatta
     3181 aggatatttt cttatgtatc ataatattag tctctcttat tattatatac tgctatcatc
     3241 attagttgtt atttatacta tttttataat atacaaattt attcagctaa cgttttgcct
     3301 aattctatac actcatcttt tgcttcttca tttgggtctt ccattgctgt aattccttct
     3361 ccaccaataa ttgtagcacc agcattttgc atacgttctt cccagtctct catccattca
     3421 caatttcccc atccatatga accaaataat ccaatctgtt tacctgatac catagattct
     3481 aattctgaaa caaatggctc catatacccc tcttctagtt gctctgctcc catagatggg
     3541 caacctaatg caaatttatc ttcttctttt aaatcacttg gtttcagtaa gctcacatct
     3601 aaaacttctg gtgtcttacc ttttaacttc acaccttcag ccacaaaatt tgccattttt
     3661 tcagtatttc ctgttccact ccaataaaca atatatattt tactcatttt tacctcctgt
     3721 agtatttttt aattaatcaa tagttagtta tagctaacta acatctaaaa aacaaaagat
     3781 ttctttatct gaaatctctt tatttttcta aatatagaat atcatagttg atattgaaat
     3841 tcaatactta ttgataatat ttatcattta tattttatat tcattttata atatcaaaac
     3901 actgtatcgt atttctaatc tttcatagaa aaatttctaa cttatagtac ttttgcatct
     3961 cttataaatc ttttatatgt ataagtacat agaaaaacac accttttcca gatgtgctat
     4021 ctataatatc gtatatttat ttttcaattc taactagcta atatgtttat aaattttatc
     4081 acttaatacc tttatacatt gatgtaaaat cttacaccta agtaattttt attatttgtc
     4141 tcgactctat ataatatagc tattttatca tcaaatcaaa aattatcttt tactattatt
     4201 cttgtattta tagagattta aatataagta tccatttcct tgttccgttc taggtattcc
     4261 aagcgctctc gtacatttta ttaattgtgc atacagttca gcttcatcta aatctctttc
     4321 aaactcctct cttgcccatt ctttaattag tgctaatgag cctgatacgt atggtgcact
     4381 catactagta ccactcataa tagcaagttt attatccata taagttgata taatatttct
     4441 tcctggagct accaaatcta ttgtagtatt tgaattacta aacttttcaa ccaaatagtt
     4501 ctcatttatt gcacctactt ctattacctc agcataactg gctggataac tatactcact
     4561 tgtactagaa tcaccatctc cattattacc tgctgcacac actacagaaa tattattttt
     4621 tactgcttgc atgacagcat tttttaaatt cttatcatct ttgtttcccc caagagacat
     4681 agatataata tcaaccttgt tatttacagc aaagttaata gcattaatta tactttgata
     4741 tgttccggta ccatctttat ttaatgcttt tgctattaat aatttacaat ctggagctac
     4801 tcccataact tcattattat aattagatgc agctataata cccgccacat gagttccatg
     4861 accattaaaa tcctcatata tatttttatt tccattactg tcatcactaa aatttgcacc
     4921 accaattatt tttcctttta aaagaggatg agatatatca caacctgtat ctattatacc
     4981 aactacaata tttttaccag tataaccttc atcccacatt cccctagcat tcatttgttt
     5041 tattccatat gggaatttat tttttgcacc tcttagattt tcatttattt catatggaat
     5101 tagtttcatt cttttattca tcatatatcc tcctaaaaat tttattgctt ataaaaatca
     5161 tgaattttat tttataatag g
//
LOCUS       DAEMGG010000190         4951 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEMGG010000190 DAEMGG010000000
VERSION     DAEMGG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.191"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            214..509
                     /locus_tag="KPK24_003575"
                     /pseudo
     CDS             214..509
                     /locus_tag="KPK24_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            917..1606
                     /locus_tag="KPK24_003576"
     CDS             917..1606
                     /locus_tag="KPK24_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2301209.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            1606..2523
                     /locus_tag="KPK24_003577"
     CDS             1606..2523
                     /locus_tag="KPK24_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2301210.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRAIVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            2596..3522
                     /locus_tag="KPK24_003578"
     CDS             2596..3522
                     /locus_tag="KPK24_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2301211.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            3519..4199
                     /locus_tag="KPK24_003579"
     CDS             3519..4199
                     /locus_tag="KPK24_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2301212.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4297..4632
                     /locus_tag="KPK24_003580"
                     /pseudo
     CDS             4297..4632
                     /locus_tag="KPK24_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT         1888 a          516 c          787 g         1760 t
ORIGIN      
        1 gaattataga atgaataact ccataaacta gaaaatttat aataatatct atatgaaaat
       61 tagaattatt ttatcacaaa aaaattaagt taaataaata tgaaattact tccaaatata
      121 cttccaaaaa catgtaatcg catatataat ataattataa aaataataaa tatttttata
      181 gtagtagtta attattaata aggaggggtc aaaatgccta gcatgattat ggaagttgta
      241 ggagtactat ttatagctat tggattaatc ttgacattca ttgaaataaa aggaaatttc
      301 ccttcttttt atggttttgg tcaaaattaa aatgctgtag gtattatagc agcaggaatt
      361 gctttaattt tctttagaaa tggcatagat acatagattc tatagctact tatggtgtat
      421 ttttagcaat agaacttgtt gtaatagttg catatttctt aataagaaaa aaatttacta
      481 aagaagataa acatttaaga actaattaac taaattatct actataaaat tggaaaatta
      541 aaaacaaata aaagtttgtt tttaattatt taaaatccaa atttgattat ttttaaagaa
      601 actatgttct gtataaaact agaatgtggt ttctttttta tgtataaaat attttttagc
      661 aaaagttaaa aggtgtttta taatataaaa tagttaattg tagatgttaa taaaagaaaa
      721 ttatgtatat tttagataaa gataagagag tatactatat tttaattctt ttttcaataa
      781 agcttattga aaagaacttt aacttagaag agtatcacca aattgcagat acaatatcat
      841 aagcagttat ttattgacaa ccctttttaa taaatatatc atttgaagta tactgacgaa
      901 aaggagttga ataataatgg acatcttatt ggttgaagat gataagaata ttcgagaaat
      961 ggtatacgat tttctgatta gtgagaatta ctctgttaaa actgccattg atggaaatac
     1021 agctattgaa atatttaaaa attattcatt tgatattatt ttactggact tgatgttacc
     1081 aaagtctagt ggttttgaag tattaaaata tatacgaagt gtgagtgttg ttcctgttat
     1141 tattactact gcgaaagata gtgacacaga taaaatgcta gggcttaatc taggtgcaga
     1201 tgattatatt acaaagcctt tttcaatcgt agaattgctt gcaagaatta aagctaatat
     1261 tagacgtgca acagtgtata gcagttcaag aaactctcaa gatatggtta ttcggtataa
     1321 ggatttgatt ataaacatat caaaatatgt agtttcaaag aatggaattg acttaaactt
     1381 aacacataca gaatttgaaa ttgttaaatt actagcgact aatcaaggac gtgctttttc
     1441 aaaggaacaa ttatacaacg ctatttggaa agaaccttat tatggaaatg aaaatgtgct
     1501 aaatactcat attaacaggt taagaaataa attgtctgat ggtatagaaa aaaataatta
     1561 tattaaaact ctttggggaa ttggctacaa aatggaggaa gaataatgga gttattttta
     1621 gttgtaattt cgattgtact atttgctctc tacttaaaag aacgaaataa atcaaagaaa
     1681 atggaaagtg attataagta tatcaattca agacttcgtg acataacaaa tgatgatgaa
     1741 attaattata ttttagtacc atctgatatt tcgattgtaa aagaaacagc agtagatata
     1801 aataaacttg ttgaaaagtt ttacagcagg caaattaatt ataatagaag tgaaaaagcg
     1861 atattgcaaa tatttacaaa catatcacac gatttacgaa ctcccatcac agtattaaag
     1921 ggttatattg aaatgctata tcttcaaagt caaaaagaag aattatcttc ttcaatgaga
     1981 gcaattgtgg aaaaaatgca aacaaattca aatgagcttg ttaattcaat aaataacctg
     2041 tttaatatgg caaaaatcca atctggagat atgatattaa atcttcaaaa atccaatcta
     2101 acacaattat gtcatgagat tattttagag ttttatgatt tgctagataa agaaaacttt
     2161 aatgttgaag ttaatattga agatagacct atatatgcaa atgttgacac agaagcatta
     2221 caacgtatac tcaaaaattt aattgataat gctataaaat atggttcaga tggtagattt
     2281 ttagggattt cactatatga aaaggaaaaa catgttttta tagatattga ggataaaggt
     2341 tcaggtattt ctgaaactga aaaagagcat attttcacaa gagcttatac tgctgacaga
     2401 aaaaaaggca atggtttagg tttggctatt tctcaagggc ttgcaaactc tatgggaggt
     2461 tcaatttatg taaatagtag acctaatgta aaaacagttt ttacggtaaa attgaaaagt
     2521 tagaaaaaag ttagatttaa aagagagaaa agacaatttt attttgtata ctgtttttag
     2581 aaaggaggga gtatgatgaa ctatatcata gagacccatc aattaaagaa aatatataaa
     2641 gataaagctg ttgttaatgc tgttaatatt catgtgaaaa agggagaaat ttatggattt
     2701 gttggaccaa acggggcagg taaaagtaca gtaatgaaaa tgttgctaaa tcttgttaat
     2761 ccaaattctg gagaaatagt tatgttcggt aagaaagtgg cagaaactga ttttgaaata
     2821 ttaaaaaaga ttggtactat tattgaaaat ccgtatttat atgagaattt gactgctaaa
     2881 cagaatcttg atttacactg tgaatatatg ggttattata acaaagaaca tatatcagag
     2941 gtattggaat gtgtcggatt gtcaaagcaa tcaaacaaga aagtttcaaa atattcttta
     3001 gggatgaaac aacgtcttgc aatagctaga gcaattctta ctaaaccaga attattgatt
     3061 cttgatgaac ctataaatgc actcgaccca gaggggataa gagaaatgcg agaattattc
     3121 cgtaagttaa atacagatta tggaataact atatttattt ctagtcatat tctttcagag
     3181 gtagaacaaa ttgcggacac tattggaatt attcaagatg gtaaacttat taaagaaatt
     3241 tctatgtcag atatacacaa atatcaaaca gattatgttg aagtagatgt agataatgtg
     3301 gagcttgctg gatacttgtt agaaaaagaa tttggaataa aagactttaa aattacttcg
     3361 gaaagttgta ttgaaatata tgatttacga aaagatgtaa aagaaatatc caaaatattt
     3421 attcaaaatc aaattggtat aaatagcatt ggacgtaaac aaagttcttt agaagattac
     3481 ttcttccaaa ctacaggtac aggaggtaaa gaaacactat gatacattta atgaaactag
     3541 agctaaaaaa agttgggcta aaaagatatt ttttattttc aatgctgggg attttcttat
     3601 gtatgttttt tgtctttact ggattgaatg ataaatctac ttcaatatat gattatgatg
     3661 ttacatttag gacaatcagt ttatttttct gtttttacta tattatatta tttgctgtgt
     3721 tgaatgtggc atatataata aatgaatata caaataaaac aatcttgatt ttattctctt
     3781 atccacttga ccgtaaaaaa cttattttat caaagttact tttgattacg actcttatga
     3841 tatcatcaat gatagttgga tatatctgtt gttttacatt cgttgttgtg ttggataagt
     3901 attttaattt agttattgga gattttcata tttcaatttt atcttattgg ataccaggag
     3961 caattaaaag tattattgta ttttgctcat taggtatatg gacattcgtt gttggaatga
     4021 taagaaaatc aatacctgtt acccttgtca gttcaattat ttttatcaat ttaagacaat
     4081 ttattttagc tggtacaaat acaactcaag acagtttgat ttctgttttt attatagttg
     4141 gtataactgt gattggtata tattatatac taaatcataa agttactgaa attgactaga
     4201 tactgtggat aggagggtag ttattaattg tatcttcttg ggataataat gatttgctat
     4261 tatatcagct attattaata ttattagagt tttaataata ttgcacaacc atttgaagtt
     4321 gagccaaagc ttgatgaatt aattgaatgg tactatagtc agagtgaagt atctattaaa
     4381 gttatagctg aatttcatta tagatttgaa ttgatacatc catttcaaga tggtaatggt
     4441 cgcataggta gatttgttat gctaaaacag atgttagaaa ataatcttcc tataaaaatt
     4501 gtatcttggg atagtgagga attgtataga aactccttaa attcttgtag tttaggtaat
     4561 tatgtccctt taattgaata tttatcaagt ttagaagatt ttagagaagt atataaaatg
     4621 ttatggaaat aattgtttgc agcaaaaaaa gtttttatat aaatatattt ttatctatgc
     4681 tatttaacgc taatttctta gttattctta gcttggacta tacccactat tttaactata
     4741 taaaatagtg gatatagttt aactatctct ttataactat ttttatgtat cttagttgtt
     4801 tcttaatatg gggacagttc ataaaactaa aagtgatttt taattttatc atttataatt
     4861 taccattaca tcagaaaaat gcttatcatt agaattttca taaaaggtac ctattttttt
     4921 caattaactg aatcaattct attagtctta a
//
LOCUS       DAEMGG010000191         4942 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEMGG010000191 DAEMGG010000000
VERSION     DAEMGG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.192"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            478..1338
                     /locus_tag="KPK24_003581"
     CDS             478..1338
                     /locus_tag="KPK24_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2301213.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKASDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1328..3199
                     /locus_tag="KPK24_003582"
     CDS             1328..3199
                     /locus_tag="KPK24_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2301214.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3219..4673
                     /locus_tag="KPK24_003583"
     CDS             3219..4673
                     /locus_tag="KPK24_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG2301215.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKIGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         1902 a          520 c          899 g         1621 t
ORIGIN      
        1 aactaaattt gaagctttac ctattaatat atcttaatag tgattaaata aaactaaact
       61 taagacttta tatgttaata tatcttaaga atttatttgt taacatacag ttcattatat
      121 ttaaaagcat tactatgata attcataata gtaaaataga taatggttca gacaatatta
      181 taaatattag tgacggtaaa atagtatcaa tttagtatat aaaccttgtc agataatcgc
      241 ataacagagc ttgtagttat agaataaaaa aatatctagg aaaccgttga cagatttata
      301 tggatagtat aacatataga atatgaaatt aaacaaaaaa gaattttaat tatggcgacg
      361 tagtcataat tttgagtatg ttactgatta gcaggcagaa ctcaagtata tatgtgaaaa
      421 atagagggat actctataca tatatgcttg agttcttttt attgaaagga ggaatatatg
      481 aaaataattc agattttaaa taataatgta gccttagtaa aaagaggtaa aaatgaaatt
      541 attgtatttg ttaaaggaat tggctttaaa aaaagagtgg gacaagtaat aaatgaaaat
      601 gaaatagaga gaacatatat actagattca tacgatatgc tagaacactt tagttatcta
      661 ctacaaaact cagactccaa taatatagtt cttattaatg aaataattga atatggtgag
      721 aaagctttaa atataaaggc aagtgattat ctaagcttaa ccttactaga ccatttagag
      781 tttttactta aaagatgtga aaagaatcaa tttataaaaa gtcctttggt atggaatgtt
      841 aaaagatttt atccaaagca ttttgaaatt ggaatgcatg cactaaaatt aattggtgat
      901 agtaagggaa ttaatcttcc tgatgatgag gcagtatcaa tagcacttca ttttgtaaat
      961 atggaggtaa ataaagagtc tcatgatagt acaattgtag agttgagaac tttagcagat
     1021 atagtttcta taataaaata tcattttaat gtagagctag atgaaacttc aacaaattat
     1081 atgagattta ctactcatct acagtacttt atccaaagaa ttatgtcgga tgaagcttca
     1141 atagatgaag ataattctat agatttatat aaacaagtaa gtcaacttta tccaaagtca
     1201 tttggaagtg ttcaaaagat aagaaaatat ataaaatcac aatttaatgt cgatatatct
     1261 ataagtgaag aaacttattt aatgttgcat ataaataggg tgactcaaag actggaggca
     1321 aaatcagatg aaatataaag atttaaatga aaatataata aaacttatag ggggaaaaga
     1381 aaatataaag tcggtagcgc attgtgttac tcgtttaaga tttactttaa atgataggag
     1441 tatcgctcaa acagaagaaa ttaaaaaatt aaaaggagta attgatgtag tttctaatga
     1501 tgttgcttat caaattataa taggtactca tgttgtagat gtatataacg agtttatgtc
     1561 tatgataggt ttaagttcaa gtgaatcaag tataaaaagt gaaagaaaaa agggaattag
     1621 aggtatcaaa ggtatattta attccatctt tgtggtagtc tcagagacta tgacttcagt
     1681 aattgaagta ctacttgcag ctggtatact tgcaagtata ttagcacttt taaatatgtc
     1741 tggcatactt cagagtgaca gtcctacata tcaaatatta gatacattaa gaagtgctac
     1801 atttcacttc ttaccagtat taattgcaat agcatcagca aaaagactta atgtaaatcc
     1861 ctatatagca gtagtgttag cagttacgtt attatcttca tcaatagatg gtgtagaagg
     1921 attaagttta tttggtataa atttacaggc tacaacttat gcaaacagtt ttattccaat
     1981 acttttagga gtatgggcaa tgggaattat aattgaaggt ttgaaaaaaa tattacctaa
     2041 agcattacac tattttttag ttccagtgtt atctttaata ataactctat cagttacatt
     2101 aactgtattt ggacctattg gtatgtggat tggtgatgga ttaaactatg catgtatgtt
     2161 tttaattgat acattgggta attggagtgt tgttgcactg tatgcagctt tacaaccact
     2221 cttaatagtt tttggtgcag caaactttac tatgcctata tctttaaatt ctgtgacaac
     2281 attggggtat gacccaattt ttatgggcgc tgcaacaata tctgatattg cagtttgtgg
     2341 tgcaatgttt ggatatttct taaaaactag gaataaagaa caaaaacaac tatttggtac
     2401 agtaagtttt agtgcactta tgggagttac agaaccttca gtgtttggtg tgtttatgaa
     2461 atatagaaga ccatttgtag cagtaatgat aggtggaggt ataggtggtt taatagcagg
     2521 tttagctcaa gttaaaacat atggaatggt ttggggatta gctgcattac caacgtatct
     2581 tgttggagga gaggtatcta actttacatt tatgataata agtgtagtag tatcatttgt
     2641 agcagcaaca gcagcttcct atttactagg aataccttca gatgaaaaag aagaagttaa
     2701 gggtgataat gaaaaagaat tagaactaat aatcaacgag aacaattcaa gaactattag
     2761 tattggaaat gttgcaaaag gtcaggtaat tgcacttggg gaagttaatg acaaagcatt
     2821 ttcctctgga gcattaggta aaggggttgg agttatacct agtgaatcaa aatcaactgt
     2881 tatatctcct gtagatggaa ctataactat agtatttcca actaaacatg catatggtat
     2941 agtgactgat gaaggattag aaatattaat tcatattggg atagatactg taaacttaga
     3001 tggaaaattc tttgaaagtc ttgtaacaca aaatcaaagg gttaaaaaag gagatgcttt
     3061 agcagtattt gaatctgata aaattataga ggaaggcttt gattctacta taataactgt
     3121 agttacaaat actagcgatt atttagatgt aatctcatca aatgatgaat cagaagaatt
     3181 attaactgta atagtataaa ttttaggagg gaaaattcat gaatattaaa gatacatttt
     3241 tctggggagg aagtattgca gcccatcaat gtgaaggttc atgggatagt gataacaaag
     3301 gacctgcaat tatggacttt gtaactaagg gctcttatga aacgccaaga gtaattactg
     3361 ataaaataga agaaaaatta gattatccat ctcataatgg aatagatttt tataatagat
     3421 ataaagaaga tatagcttta tttaaagaaa tgggatttag tgcattaaga atatctatag
     3481 attggtcaag aatttttcca aatggagatg atgaaaatcc taatgaatta gggattaaat
     3541 actatgaagg tctgatagat acattaatag aaaataatat agaacctatt gtaacactat
     3601 atcattttga attacctatg aatttagtac ataaatatgg ttcatggaat aacagaaaac
     3661 ttatagattt gtatttaaag tacagtgaga ctgtaataag aagatttgat gataaggtaa
     3721 agtactgggt tacatttaat gagatgaacc atattgaccc tcaaacagag cactcggata
     3781 tttttactta tatgatagca ggtattaagt atagtgaaat agaaaataag gttcaaacat
     3841 tggcaaatgt agggtataat atgactttgg cgggtgtaaa ggttgcaaag ttggctagag
     3901 aaatcaatcc caacaataaa attggatgtg tatttggact aaatccaatt tatccataca
     3961 attgtaatcc tagtaatgtg ttaaatgcat ttttagataa tgaccgtgat tattatcaaa
     4021 ttgatgcaat gtgcaatggg aagtttccac aatataaatt aaaagaatat gaaaaaaata
     4081 atataactat tgaagtgact gatgaagaca aaaaagcatt taaagaaggt aagttagatt
     4141 ttataggact taattattat ttttcaagtg tatctactcc aaaagaaaat ttatcaggtg
     4201 ataaatcttt atttggtggg attcaaaatc catatttgga acaaagtaag tgggggtggg
     4261 caatagaccc tataggttta agatttacct tgaactatat ttacagaaag tatcaattac
     4321 ctatcttaat tacagaaaat ggtttagggg catatgataa aatcgaagaa gatggaacta
     4381 taaatgatga ttatagaatt gaatatttaa gtaagcatct atctcaaatg aagtctgcaa
     4441 ttgaagaaga taatgttgaa tgttttggat atttgatgtg gggaccaatt gatttagtca
     4501 gtgcaactac aggtcagatg agtaagagat atggatttat ctatgttgac ttagatgatg
     4561 aaggcaaagg aagttttaag agatataaga aaaaatcatt tgattggtac aaagaagtaa
     4621 taaaatctaa tggagaagtt ttagattttt ctacggatat agtagtaggg taggaataag
     4681 ataaatagaa gttaggaact aatgttaatt agtgtctagt aaagcattta aaatagagta
     4741 tagggtcttt aaagtaaagt gtgtattctt cttaaaaata atggcaatta aaattactat
     4801 aaaaaggaga atacacattt tttattttta tacttaagta aatatttaga catattcata
     4861 gtataattta ctcattatat aaaaatagta tgatttactc actatataac tttataaatt
     4921 ttctggaaaa atgtcagaat at
//
LOCUS       DAEMGG010000192         4835 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEMGG010000192 DAEMGG010000000
VERSION     DAEMGG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.193"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            50..166
                     /gene="rrf"
                     /locus_tag="KPK24_003584"
     rRNA            50..166
                     /gene="rrf"
                     /locus_tag="KPK24_003584"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(380..1564)
                     /locus_tag="KPK24_003585"
     CDS             complement(380..1564)
                     /locus_tag="KPK24_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2301216.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1937..4288
                     /locus_tag="KPK24_003586"
     CDS             1937..4288
                     /locus_tag="KPK24_003586"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG2301217.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4310..>4835
                     /gene="nrdG"
                     /locus_tag="KPK24_003587"
     CDS             4310..>4835
                     /gene="nrdG"
                     /locus_tag="KPK24_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG2301218.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDL"
BASE COUNT         1855 a          577 c          797 g         1606 t
ORIGIN      
        1 ttctataaac ttattaaaaa cttaatagaa atattaagca taaagattat gtggttacaa
       61 tagcagagag gatacacctg ttcccattcc gaacacagaa gttaagctct ctagcgctga
      121 tggtacttgg tgggaaactg cctgggagag taggacgtag ccacgtaatt tttttgttat
      181 aataaattta gaatttaact tatatagttt aatttgataa tttgatgaaa aaaataaaag
      241 acttattgat ttttaaatct ataggtcttt tattttttat ctaacattta aattaagttg
      301 ttgaatatat cttatattac ttaccttata tattaattat cattaataca ttatatctaa
      361 ttactattca tctaaattat tatgccatta ttttatatac aaattctttt aatttatcta
      421 atccagataa gaaagtattt tcactgcaag cataggatat acgaatatat ttatccatac
      481 caaatgctat gccaggaaca actgcaacat tatattcttc taaaaattga ttacaaaatt
      541 ctatagaaaa actatcttta tattcaaatt tctcagatac tttagaaaga tctataaata
      601 tatagaatgc gccatttgga ttcacatatc ctacattatc aatagagtct agttttgatt
      661 taattaaatc tcttcttgat ttatatgttt taaccatatt gtctatgtca attgagcaat
      721 ctttcaaggc tccataggct atgtattgtg ctgttagact tgggtgagat ataagatgtc
      781 cttgtataga actcattgct ttagcaatcg ttttattaga tgcagtatat cctaatctta
      841 atccagtcat tgcagctgac ttagaaaaac catttatagt tattgttata tctttagctt
      901 cttcgcttaa tgatgcaatt gatgtaaatt caccagtgta gcatattttt tcatatattt
      961 catctgctaa aatatatatt ttattttgta tgcatacatc tactatttct attaattcat
     1021 cttttgtata aacactacca gtaggatttg aaggattgtt tattacaaga atttttgttt
     1081 tatctgttat agatttttca agttcatctt ttgttaactt aaatccattt tcttttttag
     1141 tatcaataaa aactggaaca gcattaacta acttaaccat ttcaggataa ctaacccaat
     1201 aaggctttgg taataagact tcatctcctt cgtctgttaa agctagtaaa gtatttgtta
     1261 ttgagttttt tgctccactt gatacaacta tttcatctat tgaatagtta cagttatttt
     1321 cttcaataag ttttttacag atttcctctc taagtatttt taatcctgga actaaatcat
     1381 atttagtgta atctttattt aaagaatcaa taccataaga ttttgcgtta ttaggtacat
     1441 taaaatcggg ttctcctata ctaagattta ttactttaac tccattactt tccatttctt
     1501 taactttaga actaatacct attgtgtagg atggtgttat aaagtttagt ctttttgata
     1561 acataaaatg cctcctttta aattattatt tatattatac gacaaattta aagaatttac
     1621 ataaggaaat ttgtagatat atacattaat atatataata gcctacactt tttgcctaaa
     1681 attagtgtaa aaaaaagaga aaaaaatttt aactttcaaa gttgttagaa acgctactgt
     1741 tcaaaatatc cacatagtat ttgcaatgat ttgcgcaagt ttgactatta tgtacaaatt
     1801 tggtaatctt aatatagcaa ttattaagat aatcccttta acttagttga aggggatttt
     1861 tttagactta acatactaca tatagtggta tggggattaa taaaatacaa tatatagtaa
     1921 caggaggttt ttttaattgg ttgaattcgt aaaaaaaaga gatggaagag taattccatt
     1981 taatgaagat agaataacaa gagctatatt tttagcagca acgaatgttg cagaaagaga
     2041 aggaatagta cctgattata aattatctga gcaattaact caagaagtaa taaagttttt
     2101 aaatcataaa tactcagaat cagttccaag tgttgaagat atacaagatt ctgtagttaa
     2161 ggttcttata gaaactggcc atgcaaaaac tagtgaagaa tatataatat acagaactga
     2221 aagaagtaga ataagaaatt caaaaacaag attgatgaag gcaatagagg aaattacctt
     2281 tgaagatgca gaagatgctg atataaaaag agaaaatgca aatattaatg gtaatacagc
     2341 aatgggaact atgttacaat atggaagcac tgtatctaaa gaattttgta aaacacatat
     2401 attaaaacca gagcattctt ttgctcatga taatggagat atacacatac atgatatgga
     2461 ttttttaaat atgggaacat taacttgttg tcaaatagat gttaaaaaac tattcaatgg
     2521 tggattttca acaggacatg gctttttaag agagccacaa gatataataa gttatggtgc
     2581 attagctgca attgctatac aaagcaatca aaacgatcaa catggaggtc aaagtatacc
     2641 atttttcgat tatggattgg ctgaaggtgt atataagaca tttaagaaat tctacatagg
     2701 aaatttagct aaagcattga agttatttaa aggaattgaa aataatgatg tgataaagaa
     2761 catagtatat aatacagaga aagaaacaaa tcaaaaagtt ggtcttaaga gagatgaatt
     2821 gtatctaaac ttagaaaaag agaaattaat acagactttt gatatagatg atgaacttgt
     2881 aaataagatg caaaactttg catttgaaga atcgtataga gaaactgata aaaaaacata
     2941 tcaatctatg gaagcattta tacacaatct aaatacaatg cactctagag caggtgcaca
     3001 ggtaccattt tcaagtgtaa acttcggaac agatacttct gaagaaggaa gaatggtaac
     3061 taagaatctt ctattatctc aagaaagagg tttaggtaat ggagagacac caatttttcc
     3121 aattttaata tttaaggtca aagaaggcat aaatttaaat cctgaagacc ctaattatga
     3181 tttatttaaa ttatcatgca gagtatcagc taaaagacta ttccctaatt ttagcttttt
     3241 agatgcgcct tttaatgcaa aatattataa aaaaggtgaa ccagatacag aggctacata
     3301 tatgggttgt agaacaagag tacttagtaa tgtgtgtggt tcagaaacag ttagtggtag
     3361 aggaaacata tcttttacta cagtaaattt accaagattg ggtataaaac atggtattat
     3421 aaataatgaa aaggctaatt tagatggttt ctttgaagaa ttagatgaga agataaactt
     3481 aattatagaa caattattgg agcgccttga ggtacaagga aataaaaaga tgaagaattt
     3541 tccattctta atggggcaag gtgtatggaa aggttctgat gatttaggac cagaagatac
     3601 actaaaagaa gtaataaaac aaggaacatt gacaattgga tttataggtt tagcagagtg
     3661 ccttattgct cttataggta aacatcatgg agaaagtaaa gaagctcaag aattaggatt
     3721 aaaaatagtg tcacatatga gacataaaat ggatgaagca acggacaaat ataaattaaa
     3781 tttctcctta atgggtactc cagcagaagg tctttcaggt aggtttacta aaatagataa
     3841 aaaagtttat ggagaaatta aaggtatcac agataaagaa tattatacta attcattcca
     3901 tgtaccagtt tattacaata taagtgcata tgataaaatt gagatagaag ctccatacca
     3961 tgaattgact aatgcaggtc atataacata tgtagagtta gatggagatc catctgataa
     4021 tttagaagct tttgaaactg ttataaaagc tatgaaagat ttgggtatag gatatggaag
     4081 tataaatcat cctgtagata gagacccaat atgcggtttt tcaggtgtaa taacaagtaa
     4141 tatatgccct gtatgtggaa gaaatgaaga tgaaagtgac atcaaattcg aaagaataag
     4201 aagaataaca ggatacttag ttggtacagt tgatagattt aataatgcta aaaaagcaga
     4261 agtaagagat agagtaaaac atagatagtt ttatttgggg gaataagtta tgaaaataag
     4321 aatgtcatct actataagtt atgacagcat agttgatgga ccaggattaa gaatggtaat
     4381 atggactcaa ggatgtattc ataattgtaa agagtgccat aatcctcaga cacatgattt
     4441 atgtggagga ttttatatgg atacagaaga aattataaat aaagtcaaat cattgaaatt
     4501 acaaaaaggc attacgctat caggaggaga accattttta caaccagaac cattagaaga
     4561 aatagcaaga gaagctaaaa taaatgggtt agatgtatgg tcatatactg gatttacgtt
     4621 tgaacaactg ttagataaaa aaaatagagc gtattttaaa aatttaaatt tactaaagca
     4681 aattgatatt ttagtggatg gaaaatttat agctgaaaaa aaagatataa gcttaaagtt
     4741 tagagggtca tcaaatcaaa ggataattga tgttcaaaaa agtttgaaat ataaaaaagt
     4801 gtttttggtt gagcaatata tgaaggatga tttat
//
LOCUS       DAEMGG010000193         4735 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEMGG010000193 DAEMGG010000000
VERSION     DAEMGG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.194"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            157..1308
                     /locus_tag="KPK24_003588"
     CDS             157..1308
                     /locus_tag="KPK24_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG2301219.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRLSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1354..2529
                     /gene="thiI"
                     /locus_tag="KPK24_003589"
     CDS             1354..2529
                     /gene="thiI"
                     /locus_tag="KPK24_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG2301220.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2685..3488
                     /locus_tag="KPK24_003590"
     CDS             2685..3488
                     /locus_tag="KPK24_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2301221.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFSQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3731..4372
                     /locus_tag="KPK24_003591"
     CDS             3731..4372
                     /locus_tag="KPK24_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG2301222.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1902 a          449 c          919 g         1465 t
ORIGIN      
        1 taaaaatttg taataaagtg aaaaaactag cttttcatga aataatattt tataatttaa
       61 gattgtcttt ttaataaaga ttaacaaaaa aaatactata attagtggta taatagttag
      121 agttgaagat tttaaataaa ggtaggtgta attaagttgg aaatatactt agacaatagc
      181 gcaactacaa aaccttatca agaagttata gataaaatgg tttatgcact taatacggaa
      241 tatggaaatc cttcttctgt ccatagaaaa ggtgtagaag ttgaaaaagc tataaaagaa
      301 gtaagacaag atatagctaa atctctagga gcgaaagaaa aggagattta ttttacatca
      361 ggtggtacag agtgtaataa tactataata agaggtatta ctagtttaaa taaaaaaaga
      421 aaaaatcaca taatatctac taacatagaa catccttctg ttttaaatac attaaaagat
      481 ttagaagaag atggttttga agtaacttat ttagaggtcg gaaaagatgg gaaaataaat
      541 atagaggatt taaaaaatgc gataaagtca actacatgct tagtaagcat gatgcatgtt
      601 aacaatgaaa taggaactat acagccaata ggtgaagttg gaaagtattt gaagggttta
      661 aaagaaaaaa tttatttcca tgttgatgct attcagtcat atggaaaaat taattttaga
      721 ctatctaaat acaatataga ttttatgagt gtaagtgcac ataaatttca tggtccaaag
      781 ggaattggat ttatgtatat aaaagaaaat aatcgcctta agcctatgtt aacaggtggt
      841 ggtcaggaaa ttggaattag gtcgggaaca gaaaatgtac ctggaatata tggtcttgga
      901 gaagctgtta gaatactaaa caaagattta gatgttgtta tttcgaaagt aaataactta
      961 aaaaatatat taaaaaatga aataattgat aatatagaag atataaaaat taattctcca
     1021 gaagatggag tatgtcatat attaaatgta tcatttagag ggacaaaagg agaagtttta
     1081 cttcattact tagagcaaaa gggtatttat gtatcaactg gctctgcatg ttcttctaaa
     1141 aagaaaggta gttacgtatt aaatgctata ggtcttacaa atgaagaaat aaatggaaca
     1201 ataagattta gtttatctga tatgaataca gaagaagaaa tgttagaagc tgtgaaagtt
     1261 ttgaaagaat caatttgtga cttaaggtca ataatgaaaa gaaaataaag aatactgtat
     1321 agatagtgat aataaattag tttaagaggt gaaatggtgt ataatatatt aatagtaaag
     1381 tatggagaaa ttggagtaaa aggaaagaat agatatatat ttgagaatag gcttataaga
     1441 aacataagaa atatgttaaa accaattgga agatttaatg tatataaaga gtatggaaga
     1501 atatatgttg atttagaaga ctatgattat gaagaagtaa ttgaagaagt tagaaaagta
     1561 tttgggatag tgggtgtgtg ccctggagta agagctaaaa aagattatga tacattaaaa
     1621 gaaatagctt taaaaatgtt agaagaaaaa atagaggctg gatataaaac ttttaaggta
     1681 gagtcaagaa gaggagataa gtctttcggg ttgacttctc aagaaatgag tatggatata
     1741 ggtggatact tattatcaaa ggtaggagat aggattaatg ttgatgtaag aaatccagaa
     1801 gttaaaataa agtgcgaata cagagaattt catactatgg tctatagtga tactatacct
     1861 ggatatggtg gattgccact tggaacgaat ggtagagcta tgtctctttt atcaggaggt
     1921 atagatagcc cagtagctac atggatggtt gcaaaaagag gtatggaagt agaggcagtt
     1981 cactttcata gttatccatt tacaagtgaa aggtcacagg aaaaagttaa ggatttagct
     2041 aaaatactgg caaaatactg tggtagagta aggcttcata aagttaatat cttagaaata
     2101 caaaaagcaa ttggcgagaa ttgtaatgaa gaagaagcta ctattctttc aagaagattt
     2161 atgatgagga tagcacagag actttctgaa aaaagacatt gtgatgcttt aattacaggt
     2221 gaaagtattg ggcaggttgc atctcaaact atacaaggac ttacttgtac taatgccgtt
     2281 gtagacttac cagtgtttag accacttata gcaatggata aatcagatat agttgatata
     2341 gccaaaaaaa tagggacttt tgaaacatct atattgcctg aagaagattg ttgtagtgta
     2401 ttttctccta gaaagcccgt cactaaacct agattagaga aaatagaaaa atctgaaaca
     2461 gcacttgaca ttgaaaaatt agtacaggat gctattgaca aaatagaggt agaggatatt
     2521 gagttttagc atacaagtta tttaattact aaattgaata ttgagtatat tagtagttct
     2581 gtaagtatat tagtagttct gttgaaagga tttatgtaga caatttatta atatgtagta
     2641 tttaaaaata agtactataa ttttatttaa tgggaatgat tattatgaat aaaaagggtt
     2701 ttacactaat tgaattgtta gtagttatat ctataatagg cattttagtt atagtagctg
     2761 ttccagcatt gtttagaaat atagaaaaaa gtaaagcagt tacatgtctt tctaatagag
     2821 aaaatataaa gactcaaatt gttattgcaa tggctgagga atcaagtaaa ggcaagaatg
     2881 aagtcatgaa agaggtatta gaaaacaaag atggtaagta ctttgaaaca gaaccaaagt
     2941 gtaagtcagg tggaatatat tcagcaacgt ttgatgatgg ttatgatgga ataactggaa
     3001 tagaaagcat tgcaaaagtg tatgttactt gtacaaaaca tccagatggt gttgaaatgg
     3061 ctagggatat acatcaaagt atgaaagatt tgattgcatc attttcacaa gacccttcta
     3121 taataccagg agcctcaaag ggtaatgatg attttagaaa atatttatta gacaataaat
     3181 ataaaaatgg gtggcctaca attccagatg aatttaaggc aaaatatgga ttaagtaagg
     3241 atacactata tatacaacca tatgcatata gtcctactaa atctgatgct actgtagttg
     3301 tatttgcaaa taataagact gggggtaatt ggtatacttc cctagtttac gattatgatg
     3361 aaggtagatg gtataaaggt aaaaatggta tttctgttgc aggtaggtca tgggatgttg
     3421 acacagatag tgttaagtct gtaaaaacag agattcattc taaagaggga tggggtcctt
     3481 taaattaata tatcatgttt tattaatctg gtattgatta aactatagaa aagagcagtt
     3541 tctatagatg acgaatacca gattttcatt tatatatata attgagatat attaaaagtg
     3601 tttatatttt taagtcttca catacaagct aaaaataatt ataataatat tgtaaagaaa
     3661 tttaattata agattaattt tctgaagatt tagtatttgt actaagaaat acataaaaag
     3721 gggaatagat atgaataata attttcaaga caaccaaaca ataaatttaa tacaaagtcg
     3781 tagaagtata cgaaaattta cgacagaaca aattagtgat gagcaagtaa acacattatt
     3841 acactgtgca tttgcagcac cttcaggatg taataaacag ccttggcaca taactgttgt
     3901 gcaagaccag aaacttttaa aagagattag tgatgatact ttatctagga ttcatgaggt
     3961 gagtaatgtt gagataaata aaaactttaa gttattttat ggtgctccaa ccgttttatt
     4021 tatttcttat gatgaaagta gttcatgggc tccatatgat ataggaatac ttacagggaa
     4081 tataacaaca gctgcacaag cactagggct tggaagttgt ataataggaa tggttagagg
     4141 tttatttaca cctgttgaac aaggggatat tgaaggtctt gtgagtgtat tggataaaga
     4201 agatgtaaaa gaatcagagt caataaagat gaagtttgat acaaataaaa aatacagaga
     4261 actacttgat ataccagaag ggtattctgt accatttggt attgctgtag gtattccaga
     4321 tggaaattta ccaaatgcta gagaggtagt ttataaagtt tccagagttt aggataaagt
     4381 attgcaagaa ccaatcagtt acttgaagaa ttaaaccagt aatatacaaa taaattgatg
     4441 gaagaggaag ctatttaaaa agtagtttag atttttcgtt gtaaaataat taaataaaga
     4501 ataaggtgat aagtatttta tttattatct tattcttttt tatttttgtt ttgtagaatg
     4561 ataaaaataa ggattaagtg taataaaata gaaaacaagt atgttaattt taagtatatg
     4621 ctttaaaatt tatccaagtt aaaaatttaa gatatctttt cagtataatg gaaaaaatgt
     4681 caaaatattg aaatagggaa aaaatagtga tataataaga aaaaatagag aaaaa
//
LOCUS       DAEMGG010000194         4675 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEMGG010000194 DAEMGG010000000
VERSION     DAEMGG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.195"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(1012..1209)
                     /locus_tag="KPK24_003592"
     CDS             complement(1012..1209)
                     /locus_tag="KPK24_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301223.1"
                     /translation="MQIKIMELLDKKGKTRYWLAKEIGIKQQSLNKIASNETVSIRFD
                     ILEKICTTLECTPNDVLGWDK"
     gene            1342..1650
                     /locus_tag="KPK24_003593"
     CDS             1342..1650
                     /locus_tag="KPK24_003593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301224.1"
                     /translation="MINKNILKEQQLNKIKVLKNKNDKNIKNNIFKMFNSDMEEMKSI
                     IKNELFFENIYQDELEFHMNKIKNIENIADKFYVQFMISGEEGFEITCLRTALANRSI
                     "
     gene            2323..2943
                     /locus_tag="KPK24_003594"
     CDS             2323..2943
                     /locus_tag="KPK24_003594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301225.1"
                     /translation="MNKKVEKPFVYKREYDLTKYDTKVLQKYNLEQAIYIYVGSSCAY
                     NMRARSSKWRYHIRKNNGSICYTIRNFIHNLELFYKRELELPDNIIDDKLYYSNVVEF
                     EEFETLEKAREIESTIISQYQFLDSVNYMLKQKIILLSNNDSALCMVKNGNQVSLKIK
                     NKYIEKYKNKPIMRYHIDCQFNTDGSVKSITQEFEPILELSNKKIL"
     gene            3162..>4675
                     /locus_tag="KPK24_003595"
     CDS             3162..>4675
                     /locus_tag="KPK24_003595"
                     /inference="COORDINATES: protein motif:HMM:NF020291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBG2301226.1"
                     /translation="MKGKDIQEKLITYFKFIFPKLETDEKYRAYMLVENSKTNFTRTF
                     KLNTYHELVEVVNKYNSKANIYISTCLYDNTKDKTKENILSKSVIVVDVDNADFDISE
                     IYKLAKINQVFIHLVVRSGRGWHLYFKLDKEYSNVKQLEKASKHLCELFGGDKNSLDV
                     GHVYRVPFTKNLKYDNTWSSIVNTINKTFPHTLDKLINHVPIKSSLSGNTELQVDNVE
                     QLSCFKNLVKYGISKGFRNKAMIAISNTCKYIGLSEVEALQYAYEFNSNCEVPQRKTE
                     VVKVVKSIYNNSSLIKPCKDNLNKKLCNSHCMGRLITVDDVLNKIDENIDNDIQVAFS
                     NKLIGNIKTYINKDGKKEKGTMLSVLSGAEILIISELKTFRNEIYSVDDLIDTTNLSK
                     PTIIKSLKRLEEFNIINSTKQHFNRSSKPTTIYFYNDECEQKYKEIHSIGRGLFIIRR
                     NKLITDNDMKVYIALKYLINKRMSTTQNDIELISGVTRNNINRSIKNLIEQDLL"
BASE COUNT         1914 a          468 c          709 g         1584 t
ORIGIN      
        1 aaaaaaacaa gttcaaagta caaatggaac atttaatatt tatacattaa aattatagaa
       61 aataaaaagc acttacaatt aatttgtagg tgctttttta aaacaagtta aaaatcaaaa
      121 atagtattag gaggcattgg aaatgctaga ttctaaaata gaaaagtaaa aaattgatgc
      181 atagtccaaa atacttcaaa ctattgaaat atacatattt tttgatgaga aaagtaaaaa
      241 attgatgcac tatctaaact tatctatatc tcttcttcct ttgttaggga ttaccttagt
      301 tacaatacag aggatatgct tatattaagt gagagtaata acccgtgttt aatatagtta
      361 ctagaattat agttactata gtttcgtatc tgtggcacaa taatagaata actaaaataa
      421 tgttagatgt ttacatgtac tttagttagg aactcgaatt ttacgagttg taatatctaa
      481 cactctaaat aatagctata gatattaaac gtgtacttga aacggacact tgaatatgtt
      541 tagaccactt gtgggagaaa gtataattgc cacgtgtggc taacacgcac gcgttagata
      601 taaattagtt aaatcctaat atgtggtata ccacttgaat atacattgtt gtgcaatatt
      661 gcaaatagta atagtcccaa caagttgggg aagtaacaaa tgaattttta cgttggttaa
      721 ggtgtaccca ttttggatac accctctata atttagttat gcaatatatg tttatcccaa
      781 atagtgatat taaaatactt gttttattct aatttaacaa caaaaccctt gtatgctagt
      841 tattgcaatg tttacattag ttttttatat gtcagtaagt taagacataa aactaagtat
      901 tttatgccat atttatatat atattagtag ttgcaatgtt tttataccta tcataggtat
      961 tgatttaaag ttaatttgtc agttacatgt cagtatattt gccataatag attatttatc
     1021 ccaacccaat acgtcatttg gagtacattc taaagttgta caaatttttt ctaatatatc
     1081 aaatcttatg gatacagttt catttgatgc tatcttattt aaactttgtt gtttaatacc
     1141 tatttcttta gccaaccaat atctcgtttt acctttttta tctaaaagct ccattatctt
     1201 aatttgcata aaatcctcct ccttaaatta ttgttaagag taagtatagt ttagaaggtt
     1261 ctgtttaaca ataatgtcta ttgacggtac ttacgctaca atggtattat gtttatatat
     1321 ttttgaagaa gggataaaaa gatgattaat aaaaacattt tgaaagaaca gcaactaaac
     1381 aaaattaaag tattaaaaaa taagaatgat aaaaatataa aaaacaatat atttaaaatg
     1441 tttaatagtg atatggagga aatgaaaagc attattaaaa atgagttgtt ttttgaaaat
     1501 atctatcaag atgaattgga atttcatatg aataaaatta agaacataga aaatatagca
     1561 gataagtttt atgtgcaatt tatgataagt ggagaagaag gttttgaaat aacatgtctg
     1621 agaacagcac tagcaaatag gagtatatag taaaggttta tataatatga tgacctcata
     1681 caagcctgta agagccgttt aaaagctgtc ttagtgtaat taatccttga aaatttaaaa
     1741 tacgtttaaa tggcttgtta aatatggtgg tattactatt aaatataggg ggtagttaca
     1801 tagttacatg ttgttttaaa aaataaattt aactatagtt ctttcacttc tacattacaa
     1861 aaaaataaat tgagtgtgta tgtttttagg tcaaaatgat gtttaaatat tgaaaaatag
     1921 ggatatggtg tatgtaaaat ttagaatagt gtatgtaatt ttaggcataa aaatatgtga
     1981 ttaaacctta aaaatcagga atatagtgta tgttaaaaaa gattgacata cgtgttaaaa
     2041 aaagttataa ttaaggatta gatatatatt gacatgattt attttctatg ttattatatg
     2101 tttagattaa aacatatcaa attaccatat ctatttatat attgactatt tgttaatatg
     2161 taacatgttt gtatttcaat gtaaatacaa tcttttatat cctgtgtgag ggtataccat
     2221 taaaagtatc ttatattcaa caaattaaaa aaatttaata ggaggtgtag tgtaaaagga
     2281 tttttagtta ctaagtttaa ataaaaaata ggaggacact tattgaataa aaaagtagaa
     2341 aaaccattcg tgtacaagag ggaatatgac ttaacaaaat atgatactaa agttctacaa
     2401 aagtataatt tagaacaagc tatatatatt tatgttggca gtagctgtgc atataacatg
     2461 agagctagaa gtagtaaatg gaggtatcat ataagaaaaa ataatggttc tatatgctat
     2521 accattagaa acttcataca taacttagaa ttgttttata aaagggaatt agagttgcca
     2581 gataacatta ttgatgataa gttatattat agtaatgtag ttgagtttga agaatttgaa
     2641 acattggaaa aagctaggga gatagaaagt actattataa gtcaatatca atttttagat
     2701 tctgtaaatt atatgttaaa acaaaaaata atcttgttaa gtaataatga tagtgcatta
     2761 tgtatggtta aaaatggaaa tcaagtatct ttaaaaataa aaaataaata catagaaaaa
     2821 tataagaaca agccaataat gagataccat atcgattgtc aatttaatac agatggaagt
     2881 gtcaaaagta ttacacagga atttgaaccc atattagaat taagcaataa aaaaatactc
     2941 taagccgaca agcagagtat ttagtataaa aaagatttat ttcaacaaca acactattga
     3001 tatagtgtga atgagttagc gttcattcaa gttgtttatt gactatattt aaaattacat
     3061 atattaataa gattttaaac ttatgataat ttaatgtaat ttaatgatat tattaattag
     3121 acattatgtc aagagtgttg ttctgaagaa aggagcaaca ggtgaaagga aaagatatac
     3181 aagaaaaatt gataacgtac tttaaattta tatttccaaa acttgaaact gatgaaaagt
     3241 atagagcata tatgcttgta gaaaattcaa agactaactt tacaagaaca tttaaactta
     3301 acacttatca tgagttagta gaagttgtca ataaatataa ctcaaaggca aatatataca
     3361 tatcaacttg tttatatgat aatacaaaag acaaaacaaa agagaatata ctatcaaaat
     3421 cagtaattgt agtagatgta gacaatgctg attttgacat atcagaaata tataaattgg
     3481 ctaaaataaa tcaagtattt atacatttag tagtaagaag tggacgaggt tggcacttat
     3541 actttaagtt agataaggaa tattcaaatg taaaacaatt agagaaggct tctaaacact
     3601 tatgcgaatt atttggtggt gacaaaaatt cattagatgt tggacatgta tatagagtcc
     3661 catttactaa aaatttaaag tatgataata cttggtcaag tattgtaaat actattaata
     3721 aaacatttcc acatacactt gataaattaa taaatcatgt accaattaaa tcatcactta
     3781 gtggaaacac agaattacaa gtggacaatg tagaacaatt aagttgtttt aaaaatcttg
     3841 taaaatatgg gataagtaaa gggtttagga ataaagctat gattgctata tcaaacacat
     3901 gtaaatatat tggtttaagt gaagttgaag cattgcaata tgcttatgaa tttaattcaa
     3961 attgtgaagt tccacaacgt aagactgaag tagttaaggt tgtaaaaagt atatataata
     4021 attctagttt aattaaacca tgtaaagata atttgaataa aaaattatgt aatagtcatt
     4081 gtatgggaag gttgattact gttgatgatg tacttaataa gattgatgaa aatatagata
     4141 atgatataca agttgcattt agtaataagt taattggtaa cattaaaact tatattaata
     4201 aagacggaaa aaaggagaag ggaactatgt taagtgtttt gagtggtgct gaaatattaa
     4261 taatatcgga gctaaaaaca tttagaaatg aaatttattc agttgatgac ttgatagata
     4321 caactaattt aagtaaacca acaataatta aatcattaaa aagacttgaa gaatttaata
     4381 taatcaacag tactaaacaa cattttaata gaagttcaaa acctacaact atatattttt
     4441 ataatgatga atgtgaacaa aagtataaag aaatacattc aatcggtaga ggtttattta
     4501 taattagacg caataaatta ataactgata atgatatgaa ggtttatata gcactaaaat
     4561 atcttatcaa taaaagaatg agtacaacac aaaatgatat tgaacttatt agtggggtaa
     4621 ctagaaataa tatcaatagg tctattaaaa acttgataga acaagattta ttaag
//
LOCUS       DAEMGG010000195         4661 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEMGG010000195 DAEMGG010000000
VERSION     DAEMGG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.196"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..609
                     /locus_tag="KPK24_003596"
     CDS             <1..609
                     /locus_tag="KPK24_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HBG2301227.1"
                     /translation="PTGATGATGVTGATGPTGPTGATGATGATGASAIIPFASGIPLS
                     LTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITSISA
                     YFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGI
                     VTGLNIAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            778..1539
                     /locus_tag="KPK24_003597"
     CDS             778..1539
                     /locus_tag="KPK24_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG2301228.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1848..2126
                     /locus_tag="KPK24_003598"
     CDS             1848..2126
                     /locus_tag="KPK24_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301229.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2419..2994
                     /locus_tag="KPK24_003599"
     CDS             2419..2994
                     /locus_tag="KPK24_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG2301230.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(3100..3933)
                     /locus_tag="KPK24_003600"
     CDS             complement(3100..3933)
                     /locus_tag="KPK24_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG2301231.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(4001..4630)
                     /locus_tag="KPK24_003601"
     CDS             complement(4001..4630)
                     /locus_tag="KPK24_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG2301232.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         1719 a          604 c          755 g         1583 t
ORIGIN      
        1 ccaacaggag caacaggggc aacaggagtt acaggagcaa caggcccaac aggcccaaca
       61 ggagcaacag gagcaacagg agcaacaggt gctagtgcaa taataccttt tgcatcaggt
      121 ataccactat cacttacaac tatagctgga ggattagtag gtacacctgg atttgttgga
      181 tttggtagtt cggctccagg attaagtata gttggtggag taatagacct tacaaacgca
      241 gcaggaacat tgactaactt tgcattttca atgccaagag atggaacaat aacatctatt
      301 tcagcatact tcagtacaac agcagcactt tcacttgttg gttcaacaat tacaattaca
      361 gcaacacttt accaatctac tgcaccaaat aactcattta cagctgtacc aggagcgaca
      421 gttacactag ctccaccact tacaggtata ttatcagttg gttcaatttc tagtggaatt
      481 gtaacaggat taaatatagc agcaacagca caaactcgat tcttactagt atttactgca
      541 acagcttcag gtctttcatt agttaatact gtagcaggat atgcaagtgc aggaattgca
      601 ataaattaga ttagataaga aaaggcttct aatatcgtat tgatattagg agctttttta
      661 agaatgattg atgaacatag ttttaatatt aattagtgcg catattataa aatactatta
      721 tataatataa aataataaca ttaaattgga aatatagaat tatctagggg tgatactatg
      781 aaaaataaga ttgaaatatc aatgaacaac tttgtaaaaa attatcataa atcaaataat
      841 ataactacaa agtggcaaga acttttgttt gcttatgcat ctgctgaaga tgatatgttt
      901 cagaagctaa aaagtgttgt aggaacatct catgctatgc caaaagattt tctggaaaat
      961 ggaaaaacta taattacata ttttatacct tttgatgaaa gtgtaaataa tagtaatatt
     1021 ggagggtttg aatgttctga aatatgggca agagcgtaca tagaaactaa tatgttgatt
     1081 tcaaatttaa atgatttctt atgtgaagaa ctagagaaga taggggctaa ttcatatgct
     1141 attcctgggt cgtataattt tgacagtgaa aaattggtaa gtgactggtc acaaagacat
     1201 gtagcattca tagcaggtct tggaactttt ggtttaaata atatgcttat tacagaaaaa
     1261 gggtgttgtg gaagaatagg aagcataatc acaagtttag aattagagcc aactaaaaga
     1321 ccagaaaatg aatactgctt gtataaacat aataatagtt gcaaaaaatg tgtcaataaa
     1381 tgcgtgaaag aagctttaaa agaagatgga tttaatagga ataagtgtta tgaaatgtgt
     1441 atgataaatg aaaaaaaata cacttatata ggtggatgtg atatttgtgg aaagtgtata
     1501 gttggagttc cttgttcagt aataaaccca tcaagataat ggatttaagt tgactaataa
     1561 ttttaaagat agtttataat gaaaaaattt tagttatcaa aataaatata atataaaaac
     1621 taaataaatg tgtttaaaaa atggataaat aatttgtgtt gattgacaac cagttatact
     1681 gttgatagac ttatatgtat cgctgtgata tataaggagg gttgagattt tatgtttgat
     1741 ttaatcttta tttttgcata gcaagggcta ttgcaactat gaaatttaat atataaatag
     1801 ccctttgcta ttcctaatga aaaattacta tttaatagga gagcaaaatg agctataaaa
     1861 aagcaataca tatattacca gaagaattac ttgaattaat acaggaatat gtagatggtg
     1921 aatgtatcta tattcctaga aaatctaata ataaaaaaga atggggaagt aatacaccta
     1981 ctcgtgagga gttgtcaatt agaaatatgc agatttacga agactatcaa gttggatatg
     2041 atttgcaata tttatcggaa aagtattatt tatctttgaa aagtatccaa agaattgtat
     2101 tacaagaaaa aagaaaagat atctaaggat gagccagtat agttttataa ttatactggc
     2161 tcattttctt ttgataaaag tgtttgaggt atagtttctt ttaagtggat gatataattt
     2221 aagtaatttc aataacttat acagtagcta acaaagctgg ttgtaaagta tctaataaga
     2281 agtagtagct ttttatatgt ttgaaattaa aatttattaa aattaggagg tcttgttttg
     2341 aaaaaaatac ttgattatat tccacctttt cataactttc tattaattta gtaatcttat
     2401 agaaaggaaa ttgcatttat gaaaaaaatt acttgggagg tggaatatat ctcccagtta
     2461 cctgtaatta ggtattgtaa aaaatgtgga aaaaagactg aatatatttg ttcaggttta
     2521 tttagggtta atgctcaaca taaatatttg gatatatggt taatatacaa gtgttcaaat
     2581 tgtgattcaa catggaactc aactatatat tctcgtgtta atccaaaaag cttaaatcca
     2641 gaaatactag aacaattcca tacaaatgat tataatcttg ttaagcaata tgctatgaat
     2701 atggaacttt tacatagaaa tggagcagaa gttggtattc cagagtataa aatagtagga
     2761 caagaaatta atattaatga ccttacacaa gttcatatta ttagtaaata tccatgtcag
     2821 ttaaaggtat caacattatt acgtgagaaa ctaggtttat ctaggaaggt atttgacgaa
     2881 atgttggatt gtgggattat tagaagtaac tcaggagttg atttaaagaa aggtaaatta
     2941 aattatgaaa ttatgttaga tataggcaag gagaagcaaa agtcttcaaa ttgattagaa
     3001 taactaactt aatatatttt caggaacatc atttaaaaaa atagtgactt taaaaaattt
     3061 aaagccacta ttttttaata tgttttatct atataactat tatttttcta ggttttttaa
     3121 tatttcgtaa tagtgtgttg tggctatttc acttgactcg ccttttttac aaaaagataa
     3181 tttagatagt tttggaacta cacgttcaac gacaatttta ttacctatca ttttagattc
     3241 aagtatcttc aatgcttcta aaaatctctt atcctttttt gcacgattat aaaatgataa
     3301 aacgtagaca taaaggaata ggttataatt acggaaagga tattcaactt gcataaataa
     3361 tgtgccaatt ccatagtgac aagggccgat tggtttttta atagtccaat ggtcaagtag
     3421 aaaatctaca gctttgtcaa gttttgattc tttatttaga taattactaa aacggaatgc
     3481 atttaatata ttaagagttg gtagtggatt tgagtattca gtttctggac ctcgaccaaa
     3541 actaaattta ttgcaacgcc acccaccatc agtatactgt gtatctaaaa aatgttgaaa
     3601 cgttttttgc attctaatgt cagatgcata tcccatatga cagagtacgt tagcagcatt
     3661 tgcagtatga caagggtaaa tacctccatt tggatatagt ttgaaacgtc catcttctcg
     3721 ccatgtgcta aatatcaggt cagcacattc tttaagtaaa gtatcatcag ggttcacacc
     3781 taattctaat agataaagta cactatcaag agtagagaaa ggagaacctt ttattaaacg
     3841 cttatcaggt gtagtccaat agtctgcacc attatcatac ctgtgtgata atattatttc
     3901 aatatctgat aaagattgat tttctacatt cattttttac tccatttcat attatattct
     3961 attaaatcta tcaaaaactt tattataata ttatttatat ttataattga tttattttat
     4021 ttaaaattgc atttatagct gtatcaggaa caactgctaa atcttctgca ttgaagtaat
     4081 aaactatatt agaatcaaat aatatatttg aatttggagg aaattcatca tctccaaacc
     4141 aaagtataaa taccatagat gtattaccca tatatgtaaa cctataagct aaatctccca
     4201 tgtttacttt ttcagctcca attttctcca taactaactt atatttgtct atttgagatg
     4261 taaaaacttc tgaaagccta tatagagtcc tgtttaaaaa atttggataa taaacattgc
     4321 ctcctggtat ttctttaaaa gttacatatt tattagttct aggaatacct ttagcattga
     4381 ttaaatatct aactatcaag attttaagct caaagttatt gcaaaggcta ccattactat
     4441 tccaaacttc tccacttgga tagtctataa tatattgtct atcaaaaaaa ttgagtgtga
     4501 atctatttaa atcattattg aaaggtatat tacttaaatt actaattgta tgagggtcta
     4561 attctttgag tttttttcga acatagtcaa atggaacatt ataattattt tttgttatct
     4621 tttcatccat aattttttcc tccaatttaa acaattaaaa g
//
LOCUS       DAEMGG010000196         4590 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEMGG010000196 DAEMGG010000000
VERSION     DAEMGG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.197"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(709..927)
                     /locus_tag="KPK24_003602"
     CDS             complement(709..927)
                     /locus_tag="KPK24_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301233.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(928..1335)
                     /locus_tag="KPK24_003603"
     CDS             complement(928..1335)
                     /locus_tag="KPK24_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301234.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(1421..1909)
                     /locus_tag="KPK24_003604"
     CDS             complement(1421..1909)
                     /locus_tag="KPK24_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG2301235.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(2114..3235)
                     /locus_tag="KPK24_003605"
     CDS             complement(2114..3235)
                     /locus_tag="KPK24_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG2301236.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTQKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(3608..3778)
                     /locus_tag="KPK24_003606"
     CDS             complement(3608..3778)
                     /locus_tag="KPK24_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301237.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(3950..4393)
                     /locus_tag="KPK24_003607"
     CDS             complement(3950..4393)
                     /locus_tag="KPK24_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2301238.1"
                     /translation="MIEIKTIEINKKSQIDLLILADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         1538 a          748 c          393 g         1911 t
ORIGIN      
        1 ctataagttt agttcttcat ctataaatca tttatttttt aatctataag tttggttttt
       61 catctataaa taagtcattt attttttaat ctataagttt agttcttcat ctataaatca
      121 ttcatttttt aatctataag cttggttttt acctataatt aattcattct ctaatctata
      181 agcttggttt ttatttataa gtcatttatt ctctaatcta taagcttggt ttttatttat
      241 aagtcattta ttctctaatc tataagctta cttcttaatc tataagtcat ttattctcta
      301 atctataagc ttacttctta atctataaat cattcatttt ttaatctatt agcttgtttt
      361 ttacctataa ttaattcatt ctctaatcta taagctttct ttttcatcta taaatcattc
      421 attctttaat ctatgagctt ggtttttcat ctataagtca tttattctct aatctataag
      481 ctttcttttt catctataaa tcattcattc tttaatccat tagcttggtt tttcatctat
      541 aagtcattta ttctctaatc tataagctta cttcttaatc tataaatcat tcactcttta
      601 atccattagc ttggtttttc atctataagt catttattct ctaatctata agcttggttt
      661 tttacctata attaattcat tctctaatgc ataagtaata catgaaattt aaatattatt
      721 ttcttcaata aattcaaaca actcttctac agttgtatca aataccttag caatatccat
      781 tgccagtttt aaagaaggat tgtagcgacc attttctaaa tgtacaatag tttcacgtct
      841 tactcctact gactttgcaa gttcaccttg tgtcataagg ttcttttctc tataggattt
      901 aatttttgtc tttagtaata ctgacaccta attgtatatc ccctttctgt caaaatatat
      961 aaatagaatt aacttcaaca aggattgaat aaacaaaatg actaataaag acattcctat
     1021 atttaaattt gacacattta tgtccaaagt tacaagaaat atagatacta tagctacaga
     1081 aaaaaacaat acttttattg caatatcatt aactcttgat agtatacatt ttgatgcctc
     1141 atcaattaaa tcatcatact ttttattata gacccactga gctcccatag ctaataccca
     1201 aataattata ggaagatatt caatactact tcctttaaaa aaatacttat ttactaatgt
     1261 agctccaata caataagaaa ttcccaatat ttctgaaata agtgttgatt taataccagt
     1321 ttgttgttta gacatataaa atccccctta aaaagttatg tatttataac acctcttgtt
     1381 ataaatatat aacttttttg aataaatttc aataaaaata ttattttgca acaaattctt
     1441 ttattaattt tacattagat agaggcactt tagatgtcac tccttgcaat gtcaaatctt
     1501 ctatattaac ttcatttaat tttgaacaca gttctaagtc aaatattctt tcccaactta
     1561 ccttcttttc aatttgttca tcttcataaa gctctacagt aatatccatt aaaggtaaat
     1621 gccataaatc aaattctgat aataacacat tgtcttcact tatttctatt tcaagtaaaa
     1681 cacctttttt tcctttttct aaataaccac tacatcttaa atcaggtctc ttcgtccaaa
     1741 cccatatcat actatcatca aattcatata ttctatttgt catttgacta tacatccatt
     1801 tgtatggaat atcaaagtct tctaatataa aagaataatc atttgataga tatttattgt
     1861 ttttattgtt attccaaaat tctaaagtct gtatagtata aaatttcata tataaatcaa
     1921 cccctttgtt tttctataag aaggagatgg ttcaaacttt tattttgaat tatctccttt
     1981 atttatacac ctctttgtac ctattattta tatatctctt tatatctacc atatttaaat
     2041 aacaaagttt tatattaatt taaatttaaa agaggaagct ttgtatttat ctagtgccat
     2101 ctcaatcata gtattagttt tgattataga ctttttcaac ctctgattta ttcaaatcat
     2161 ctataatatc gcatatttca ctcaaattgt atcctttatt ttttaatttt atgatgatat
     2221 taaatttctc ttctcttttc ccttttgcaa tacctttttc gatacctttt tcgatacctt
     2281 tttcaatacc ttcttgaaaa atttcttctt taaaatttct acttttattc ataatatacg
     2341 cacctctctt taaatttaat aaatagtcct cattactacc taattctttt aatctatcaa
     2401 gtgctctttt aataacataa tccgttttag atagtattct aatatctata tgagaaggat
     2461 tatcaataag tttaccccat attagaactc tgtttgctat acaactggat ttacgaattt
     2521 ttggaagttc tataaaatat aatgcagttt tactagaaaa aatatctgga tgcccgtcca
     2581 ttgttaatct caggcaattt acataatctt ctatgtgatt atattttaca tgatttagta
     2641 tatgtattcc aacagttttt ccaatttctt tatattcttt accttcttct aactttctac
     2701 catgtacacg actaagataa tattcagctc tttccaaata atcctcttca agagctcttt
     2761 gcatctcaat atttatatgg ttacgttctt catcttcagc caaaatatca agtctaggtc
     2821 ttttctcacc aatgtaatta attgttgggt cggtttcaac cttaattatt ctttttattt
     2881 gagtgaactg tttgatattc ctattagcat atggagcatc tttgaggata tcatttataa
     2941 aatttgcaag taaatcgtaa tcattattta aagtaaacaa tgatttaaat acctcatcat
     3001 actcaggctt agcataccca tcttcagtta caacttctcc aacattttca ctagtcaata
     3061 tttcttttgg aattaaatcc ttgattttat ctaatttttc attagaaaaa tcacctctac
     3121 taagtatgtc atttaatttt tgggtccatt ctctgtcctt ttcttcaatt atagttcgtt
     3181 tatactcttt tgatgtttta ataatcttag atttttcttc taatttttct ttcatatcaa
     3241 cacctctttt ttattattac ccatttcttc ataccaaagt ctgaacttct aagtaaaatt
     3301 tgcattattg aaataagaac cttataaact tatatatcat tactaaagtt aagactttaa
     3361 agagaagcac ttagattaca atagatttta gatagctttc tttttaaaac tattgttgat
     3421 aaatgaatga atataagggt atgtattaaa atagagatac tcatcttttt cattgtttaa
     3481 gtaatgaaaa tatattttat ttaatacaaa aaagagtgcc ttataaacta aaagttcact
     3541 tcgagacact cccttacatt ttgtttctta aactttacaa tattacatac ttatttatta
     3601 atttaatcta tgcatactta acccaataat aaagtacata tatccaactc attaagccat
     3661 gaaaaatagc ccatggaata gatttccatg cagtataaga aataatcatt gctaaacaag
     3721 aaccaaaaga aatacccttt ttaacagttg attttatagt attattattt gaatccataa
     3781 ttaatcctcc aaaattcaaa ataaacaagt tattatatat cttatttgaa tctaatacaa
     3841 tataacagct tctaaataaa ttatactctt attatacata atttataaat tataattccg
     3901 ataattatat gaattaatta attatattaa cctaaaaata ttatcctatc taaaagttat
     3961 atgatagata caacatgtca acacattgta attctccttc aaagattggt tctggataat
     4021 tatctacaaa aaagttttta attttatgtg aataaacaaa tccaaacttc tcataaaaag
     4081 gaactccact ttcagatgta ccaacaacca ttgatgaata ttttttacta taaacatcta
     4141 aaagatgata aatcatcttt gaggcataac ccatattttg aaaatgctca tatgtagcaa
     4201 tgttttttaa ttcacatatt tctttggaaa tttcatttac aactgctata caagtaagtt
     4261 ctttattatc atacaatgcg tacatagttc ctttattgag atatttatca atcatttctt
     4321 cacatgggtc agctaatatt aaaaggtcaa tttgactttt tttatttatt tctatagttt
     4381 ttatttctat cataagttct ccaccctcac tttattcaaa ccttaattta acaaacttct
     4441 tacagtactt atttagttca ttttgatata tatttttact ttttaaaata tagtgataat
     4501 tccctagaat attaatatta ataataaatt ataatatcag ctctagcaat tacctagaat
     4561 atcaatacta atattcatct ataatattag
//
LOCUS       DAEMGG010000197         4560 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEMGG010000197 DAEMGG010000000
VERSION     DAEMGG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.198"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..673
                     /locus_tag="KPK24_003608"
     CDS             <1..673
                     /locus_tag="KPK24_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2301239.1"
                     /translation="MLNHLKENIELYMKQKNLLYNIELFNKVEDLLCNHEKFNIVFLD
                     IQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAFEVDAINYLIKPLSNEKLYKT
                     LDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVIKRKIYVHLKKETVDYYETIE
                     NLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGDRIPVSRLRQQIFSKVMLEFM
                     KNRRI"
     gene            675..2033
                     /locus_tag="KPK24_003609"
     CDS             675..2033
                     /locus_tag="KPK24_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2301240.1"
                     /translation="MINLFSSDLFLKYSFLFSFIVYRILMVIVQYYFLISFTNVNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            2072..2557
                     /locus_tag="KPK24_003610"
     CDS             2072..2557
                     /locus_tag="KPK24_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301241.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(2820..3056)
                     /locus_tag="KPK24_003611"
     CDS             complement(2820..3056)
                     /locus_tag="KPK24_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301242.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(3211..3435)
                     /locus_tag="KPK24_003612"
     CDS             complement(3211..3435)
                     /locus_tag="KPK24_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2301243.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(3532..>4560)
                     /locus_tag="KPK24_003613"
     CDS             complement(3532..>4560)
                     /locus_tag="KPK24_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG2301244.1"
                     /translation="FFTTIGLEISINLIKKGGSVLFRYWILCGVLAFCQNILAITISK
                     IIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVGVGLSAATIGLIMGGILG
                     CPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNKNRIKNNNFSETITPSVF
                     LEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVIFRNLNDKINILELNFKL
                     IDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVIFIILFSIFICFRVLGKD
                     FDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLVVPLVSGFLLDAISIPCI
                     LFFINILT"
BASE COUNT         1774 a          547 c          604 g         1635 t
ORIGIN      
        1 tatgctaaat catttaaagg aaaatataga attatacatg aagcagaaga atttattata
       61 taatatagag ctttttaata aagtagaaga tttactatgc aatcatgaaa agtttaatat
      121 tgtattttta gatatacaga tggaagagat taacggaatg caggcagcta gaaaattaag
      181 gtcatatggt aatgagtgct ttataatttt cgttacagtt ttaaatgaat ttgtatttga
      241 tgcttttgaa gtagatgcta taaattatct gataaaacct ctatctaatg agaaactata
      301 taaaactttg gataaaatag ttacaaaact tgatgataat gaaagtaatt atatcacaat
      361 acaaaaaggt tattcatatt ggaaattaaa acttgctgat attttatatt gtgaagttat
      421 aaaaagaaag atttatgttc atttaaagaa agagactgta gattattatg agactattga
      481 aaatttagag agaagtttac caacaaattt ttttaggtgt catcgtagtt atataatcaa
      541 tttacaatat gtatatggat atgaaaatgg taatgcagtt atggaaagtg gagatagaat
      601 acctgtttct agactacgtc aacaaatatt ttcaaaagtt atgcttgagt tcatgaaaaa
      661 taggaggata taagatgatt aatttatttt cttccgattt atttttaaag tacagttttt
      721 tattttcttt tattgtatat agaattttaa tggtaatagt gcaatactat tttctaatca
      781 gttttacaaa tgttaattca aaattttggt gttttatttt atattcgata atttcaattt
      841 ttttaatcat tataaataca tattttaatt tatataattt attttcttca cagattctat
      901 tttttcttat gactttttta tttgtaaagt acataataaa acaatctact gttttatcta
      961 tagtatcaac tttgttagta tatttaattg aacaattagt atatggattt acagctccat
     1021 taaattactt gatatttact agtgatataa atacagatat ttataaattt gatataaata
     1081 tgggactatc ttctttaatt actattataa tagctggatt tatatattgg tatagttcta
     1141 aaaagtttaa tattaaggtc atgaactttg ataaatatat agcaattttg atgattccat
     1201 tgttgcttat aatattattt atgcaatcat ttgaatatag ttcaaatata aatatagata
     1261 caagtcttgg gattgtaaag ctgctattaa atacttctat aactgaagaa attcaagctt
     1321 ttcttttttc tataattgga actatatgtg tatttttttc tctatttacc tttaagaaac
     1381 taattcaagc tctcgaagat gacaaggaga gggcaattat gaatcaacag attcatgctc
     1441 aaaaaaacta tattgaagaa gcgaaatcac gattatcaca aactatatcc tttagacatg
     1501 attttaataa tcatttagct attgtcaatg gattgttgaa aaaagaccaa attttaaaag
     1561 cacaagatta tttaaataag cttgaaaaag taacaagtga cctatcattt tcttgtaata
     1621 cacagaatat tgttattgat gctcttttca acaataaatt aagtatagca aaacaattag
     1681 gcatagaggt agattgtgaa gtaattatac cccaaagcac taacatgaac gattttgacc
     1741 tatgtatagt gtttgctaat gctattgaca atgcaattaa agcttgtaga gttgtagata
     1801 aacaaaatag gtatttaaag ctttccacta agttagaggg tggatttttt atgatagaaa
     1861 taaaaaatag ctataatcca ataaataatt atacaagtgg ctcaggtata ggtcttttaa
     1921 atataaaaga aattgctaaa aaatatcaag gggctataag tatagataag acatcagatt
     1981 attttcaaat aaatgtattg ctggttattt cattacattg cgctagtatt tgataacaat
     2041 ttcattgaaa atagtatttg taagctatat tatgtacttg aatagagaaa gtttgatata
     2101 tagaaatgag gaggttacta aaatgaaagt tggaaatata aatggtcaag caccaattac
     2161 aaatggttta gatagttgtc aaagtgaggt acaaggctta gaacaaaaga aaaaatcatt
     2221 acaggatgaa atagagaaag taagaagtga taaaagtatt gatggtgaaa cagccaaatt
     2281 aaaaatagaa gctttaaaaa aagaaatgga agagattgaa aataaaattc aagagttaaa
     2341 aggaaataaa gcaaaaaaaa caaataaaga agaaaataat gtttcagaag taaataataa
     2401 tctattagag agaaaaaaag aaccttctaa taatgaaaat gtaaaacaat tatatgatgt
     2461 ttttgaaaga agcaatgtag acgaacaatg ggataatact tatcgtgttc tccaagatga
     2521 taaaaactta aaagtaaaat ttaatagacc tatatagttt atttataaaa atagttaata
     2581 ttttaattat caccactttt tagaagaata cacactttat tttaaagacc cacatattta
     2641 tttacagtta gcacatttat atgtggtttt ataactatta tttacaagtc tatattgata
     2701 acttttacat agtatataat gtttaaatac ttattcaaat ttatctatac ataaacaaaa
     2761 aaagagattt taaaatctct ttttagattg ttgacttatg gctttttgat ttaataaaat
     2821 caagaagccg tttgaatttt atctgttggc tgataccctt ctgatattga aacaccattt
     2881 ttgtttccaa aatcgtttaa aattaagaac ttattatgaa tgactatttc attatgaaca
     2941 ataagcagat aacatgaatc ttcaatcaat gttttaacag tagcattaaa tgcttgctta
     3001 tacagttcta tcgtttgttt gaactgtcca ttaaagtaaa tatgagtcaa taacataact
     3061 atatctacac aaattaatat aaatttttta tactgtacat tatctaactg ttgattatct
     3121 gtattacaac atgattgaat ttaaaaagcc atcaacacat agaatgttga tggcctgaaa
     3181 atagatttaa aatctctttt ttattatata ttaattattt tttaaattta tattttttgc
     3241 atcagatttt ctaatagcta agtcaaaccc tctaaatcta atcactatag ggtcattaag
     3301 aggagctatc tttttcactt caacttcagt gccatctatt agtcctaagg ctagaagtct
     3361 cttcataagt ttttcatttc cagctatatt gtctataatt cccttttgtc caagttttaa
     3421 gttgtaaaca gtcattatta acacctccta atgaaaatca ttatcaaact ttatatttat
     3481 aatatataac aaaaagagca aattgtcaat atataacaaa aaataaacat actaagttag
     3541 tatgtttata aaaaatagta tacatgggat actaatagca tctagcaaaa aaccacttac
     3601 taatggtaca actaaaaagg cttttggaga atcaccatat ttttgtgtca aagaactcat
     3661 atttgccaga gcgttcggag tagctcctat tccatgtcca atcaaaccac ttattatgat
     3721 ggcagaatca aaatcttttc caagtactct aaagcaaata aatattgaaa ataggattat
     3781 aaagattact tgaaaagtta ctattaagag aataggaggt aataatgtag ataattcaaa
     3841 taaatctata ttcataagtg ataaagttaa aaaaattccc aatgaaagtt cttttaaaaa
     3901 atctataagt ttaaaattta attctagtat atttatctta tcatttaaat ttctaaaaat
     3961 aacagccgaa aacatacaag ttacaacaga gggtaatagt atattaaacg ttatatagaa
     4021 gcatctactt ataatttctc ctgtatttat acaaataaat atgagaagaa cctgttccaa
     4081 aaatacagat ggtgttattg tttcggagaa gttattattt tttatcctat tcttatttct
     4141 aaaaaattta cttccaaatc tctttagatt tctattatat ctaggaatcg acaagtctat
     4201 atttgatgat ggttttaatt tatatttatt tattagaaat ttagctacag gacatcccaa
     4261 tataccaccc ataataagtc ctattgttgc agcagataat ccaaccccaa ctgctccttc
     4321 tataccgaga ttttctattg taagtccaaa tgcagcagaa taaccatgac caccttccat
     4381 agatacattt ccacacataa gccctaaaag aggttcaagt tttataatct ttgatattgt
     4441 aatagctaat atattttgac aaaatgccaa tactccacac aaaatccaat atctaaataa
     4501 tacactacca cctttcttta taagatttat gcttatttca agtcctatag ttgtaaaaaa
//
LOCUS       DAEMGG010000198         4559 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEMGG010000198 DAEMGG010000000
VERSION     DAEMGG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.199"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            144..1283
                     /locus_tag="KPK24_003614"
     CDS             144..1283
                     /locus_tag="KPK24_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG2301245.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSLNSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1309..1497
                     /locus_tag="KPK24_003615"
     CDS             1309..1497
                     /locus_tag="KPK24_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301246.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            2281..4065
                     /locus_tag="KPK24_003616"
                     /pseudo
     CDS             2281..4065
                     /locus_tag="KPK24_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1972 a          513 c          734 g         1340 t
ORIGIN      
        1 cttattctta aatttttgca ttatttatta aaatttatta tgctttaaca ctaaattgag
       61 tttgataatt ataaaacaaa taatcaaaaa tagtgtaaaa attaatactt tttgttaata
      121 ttaatagata aagataatct aaggtggtga ttaaactgac aatagaagaa tacaaaaaca
      181 aatactttac tggaaataaa atcgtacttt ctctaaatag ttttgataat aaagaactat
      241 actatcatga aactgaaaat atattaaatt taataaatga aatagataaa ttaacatcaa
      301 gtaagatatt taaaacaata gaagataaaa atgatttggc actagagtct tattattcta
      361 gtgtaataga aggtgctttt tcaactagaa agattgcaaa atctataatt agaggaaaga
      421 tgaaaccaag taataaaagt gaatatatga tttacaataa tcatagagcc ttagaatatg
      481 gattagacaa cttagataaa ttatatagtc acaaatttat atatgatttg catcatatat
      541 tgggcgaaaa ctgcttggat tcagaagaat atgagtatag aactgaaaaa gtatatgtat
      601 gtgattcaaa aggtgagatt atacatacag ggcttgaacc tttgaagata tatgacttta
      661 tgagtaaatt aatagatttt atggaaaata gcaaagtaag taatttaatt aaaagtgcta
      721 taattcattt ttactttgta tatgttcatc cttttagtga tggtaatgga agaacatcaa
      781 gagccttatc atatctatat ttgattgata aaggatatga tactttcaag gatttttcaa
      841 tatcatatat gatatctaaa aacagaacta agtattataa ggctatactt gatgtagaaa
      901 acaaaggaaa taatttaact gtatttatag aatttatgct caagtctata atacaatcta
      961 ttaatgagat gcgtaatatg catgatagaa aatctttaga aagtatatta aaagaagaat
     1021 tgtttgaaaa tgacataact ctatcagcaa cagaagaaca tatattaaaa tatatttgca
     1081 ataaagataa ctattctatg acattagaaa attatataaa aaagaataaa agtagatact
     1141 taaaggctgg aataaaggaa attgaattag ttgaccagtt gatggaagtg tttaataatc
     1201 ttgaagagat agagattttg tctaaagaaa aaaatatata taaagttaat gataagtact
     1261 taaaaatgct tgatatagac taatttagat aatctggaga gggaatatat gaataataag
     1321 aaaaaggcat ttttgatttc tggggctata aacgtaattt cattaattat tgttttttca
     1381 ttgtttcatt ataatattat agaaaatagt acacaaaata tgataataac tgtaatctta
     1441 atttttattt ttgaaatgat aaaaataaaa atcatagaga aatactataa actataaaca
     1501 agaaatatct tttctaagag catattatac aatatgctct taatttagtt atatagattt
     1561 aatataaata atgtattttt tgtagatgat ttatgtaaga tttatgacca atttgtgtat
     1621 gatttgtgtt ctttaatcat gacaatatta tattgtaaaa aataaattta ataaaaaatg
     1681 acttggaatt tatattctag gtctttttta tataaaaatt taataagttt caaatgttgg
     1741 taactttgac agtagaaaac gtctattgag ataacgaaga ttctaattga gggggagata
     1801 tattagcact taaagtagta tatgcaacag atataatctt tataaaagta atttgaatta
     1861 aaatgtaaat ataaagaata attaaaaaaa tatcgcaatt atttgttttt catatggata
     1921 aaattggtta tttaatatat aattacaagt ataaataata aaatttattt gtaaaaagag
     1981 tagcactttt aattctaaag gctacttttt ttatacaaaa ccctattttt tataacaatt
     2041 cattaacata aaaatcaaac ttaccttaaa atataaattt acaaattttt caatcaataa
     2101 gaaaatttct ttattcctta cataatgact gcaatgtaac aaaataatta caaattaatt
     2161 atgaaatcat tatagttaga aacctcttta aaattagata ttaagtatcc tttagaatta
     2221 gaacgggaac aaagttaaaa aattaagtaa tatcaaatgg acaaggggga agaaaataaa
     2281 atgaaaatac caaagaagat tgctgcaatg cttacagtta caatgatagc tggaagttca
     2341 acagcaggca tagctagtgc acaaactgtg gcaacaaatt taacagggca agaaagatat
     2401 gaaactgctg ttaaaataag tcaagatgga tggaaaaatg ctgatgaggt agtaatcgta
     2461 aacgactcat ctatagcaga tgctctatct gcaacaccat ttgcaaaagc aaaaaatgct
     2521 ccaatacttt taacaagtaa agataaatta aatgataaga cgaaagcaga aatacaaaga
     2581 ttaaaagcta aaaaagtata cttaataggt ggaacttctg tattaagtac aaatatagaa
     2641 aaagaaataa aaaatttaaa aatatctttt gagagaatct ctggagctga gagatatcaa
     2701 acatccttag aattagcaaa gaaattagat gctataagtg atgttaagaa aatagctgta
     2761 gttaatggtg aaaaaggact tgcagatgct gtaagtgtag gagcaccagc tgcacaaaac
     2821 aatatgccag taatacttgc tgattctaaa aatggaacag cagttgcaga caaatttata
     2881 aaagatgctg gaataactca atcttatgta gtaggtggag agagttcaat ttctgaagct
     2941 gttaaaaata aactaccaaa tagtacaaga ctaggtggaa cagatagaaa tgatacaaat
     3001 gcaaaagtaa taaaagaatt ttataaaaag acagatttaa aaaatgctta tgtaactaaa
     3061 gatggtatga ataaacaaga ccaacttata gatgcactag cagtaggtgt attaggtgct
     3121 aaaaatcaat caccagtagt tttagttgga aagaacttat cagcttcaca aaaatctcta
     3181 gttaactcta agagttttga taagataact aaagttggtg ggaatggtaa tgagacagca
     3241 tttaatgaaa tgaagtcatt acaagaagta aaaacagttg aagctaaaac aatttctgaa
     3301 ttaaaatcag ctatagataa agctactgct aatgatgtta taaactttaa gccaactagt
     3361 gaggttaaag aagcatttac aatacaaact gataaggcag taacagtaaa cttaaatggt
     3421 acatatacaa agactgtgac tatcaacatg cctaatggtg atgtaaataa ctatgcaaaa
     3481 gtggatgatg tagttataga tgatgtaaaa gatggaactt ttgttaatta tggaaaaata
     3541 actaacttaa aggttaatga taaaaatgga gccaagatag aaaataattc taaaggtgag
     3601 attggtagtt taacagtagc atctggagca agtcaagtaa aagtgactaa tggtggtaag
     3661 ataactacag ttacaaataa ttctaaaggg actactatag ataataaagg tacaataagt
     3721 agtgttaagg gagataattc tccaacaata agtggaaata gtccaagttc taactcttca
     3781 ggaggaagtt catcaagtgg aggttcttca catggaggag gctcatcttc aggaggaagt
     3841 tcttctaatc aaacatcagt aaataatgaa gcagcaaaaa taacaagcgt gcctacacca
     3901 gcaaaagatg caactagatt aactatgcca tcagtatcaa gtggatatac tatagcaata
     3961 aaaacatcaa gtaatgagag tgttataaaa aaagatggaa caataatacc tccaaataca
     4021 gcaacaacag taaaattagt atttacggta acacatacat caagtggtaa aacagcagat
     4081 acaaaagaac tatctgtaac agtaccagct aaatcaacag atgaagaatt acaagcagca
     4141 ttagacaatg aagtggcaaa aataacaagc gttcctgcac cagcaaaaga tgcaactaaa
     4201 ttaactatgc catcagtatc aagtggatat actatagcaa taaaaacatc aagcaataag
     4261 ggtgttataa aggaagatgg aacaataata cctccaaata cagaggaaac agtaacattg
     4321 gtatttacag taacacaaga atcaagtggt aaaacagcag atacaggaga aatagatgta
     4381 gtagtaccag ccaaatcaac agatggggaa atacaagcag aagtacaagc tgaggcagat
     4441 aaaataacaa gtgtaactca gccaacacaa gatgcaacaa cactaactat gccaacagta
     4501 gcaagtggat acacaataaa gataaaatca tcaacaaatg aaagtgttat agcgaaaga
//
LOCUS       DAEMGG010000199         4541 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEMGG010000199 DAEMGG010000000
VERSION     DAEMGG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.200"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            201..914
                     /locus_tag="KPK24_003617"
     CDS             201..914
                     /locus_tag="KPK24_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG2301247.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            1032..1901
                     /locus_tag="KPK24_003618"
     CDS             1032..1901
                     /locus_tag="KPK24_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2301248.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANINLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKELVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(2056..3201)
                     /locus_tag="KPK24_003619"
     CDS             complement(2056..3201)
                     /locus_tag="KPK24_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG2301249.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(3534..4310)
                     /locus_tag="KPK24_003620"
     CDS             complement(3534..4310)
                     /locus_tag="KPK24_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG2301250.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT         1663 a          572 c          709 g         1597 t
ORIGIN      
        1 ctatttatgt tataaagtta taaatataat atagaatcta ttattctgta ttattaagtg
       61 gggttaagcg ttataattaa ttataaatat tgaataatct aaatggataa gataatggta
      121 ttaaaaatta tcttaaaagg tgaaataatc tgtatttaat gtagtacagt tatatcttta
      181 aagcaaggtg gtgaaatgat ataaataaga tatctaaaag agggtttgat gatagtgata
      241 aaagatggtt ttcaagtaaa gaattaacaa gacttataaa ggctaaagaa gaaatagaat
      301 ggttattaaa tagggattac aaaatagacc cagtcgttac ttttgttggt gatagatatc
      361 agttttctat aagacaaaga gatgcattaa aaagggcagt ctgtactgat gaaaaaaata
      421 ttataagaca atcaaagagg ctttctctag ataaaatcaa agaagatacc gtaaatatag
      481 atggctttaa tttgataata tccatagagg taggattatc tggtggtact ctagtaatag
      541 gaagtgatgg caatataaga gatttagcag gtcttagggg aacctataag attatagata
      601 aaacagaaga agctttgaat ctgataggta aattttttaa taaatacaat gcacagaatc
      661 ttaaatttta tttagatgct ccagtttcta attcaggaaa tttaaagtat aggatattag
      721 agcatgccaa aacttgggga attgaaactg aagtagaatt agtaaaaaat gcagatgttg
      781 tattggaaaa attagataga gtagttagta gtgatgcagt catagttgac aagtgtataa
      841 gttattttaa tgtggctaga ggtataattg aagagtatat taaagaatgt aatatcataa
      901 atttaaataa atagataaat ataggtgaga atatataagt atatgtagca atattcttag
      961 taatacaagt aaagtttgaa aataggagaa cgaatacata tcgaatttca taaaatgttg
     1021 taaaaagaca tatgagagga tggatatgtg tgaaattaac taaactaaat attaaaaaat
     1081 atagagcacc tatatataaa tatgctttag caaatataaa tttgaggtct gctaaatcta
     1141 ccaattcaag tatcataaca gtaatacctc agggagccaa aatggaagta ttggatgagg
     1201 aagatgactg gattaaggtg atgtacaatt ctcaagaagg atatgtgtat aaggagttgg
     1261 tatcagtaag cgaatatgct tggagtaact taaacttaag agaagataaa tctacaactt
     1321 ccaatataat tacagtaatt cctgaaaaat ctagggttga agtccttcaa gtagatggag
     1381 attggagtaa ggttgtatat gatgataaaa taggatatgt ttttaattat tttttatcaa
     1441 ttgatgggaa taaaccaaat gaattagatt ataagtattt ttatactgac atgattaaat
     1501 ttgttaacga aaataatatt aaaagtacta gcgattatct aattgtaaca gaccttagaa
     1561 ataaatatac ttatattttt aagaaagata atggaggatg gggacaactt tacaagtggc
     1621 aatgtactat aggcaagcct gagactccaa ctataacagg tatattttat ataagtggaa
     1681 gaaaaccttc ttttggaacg gatgagtatt cagttaagta tgctactaga attaaaggtg
     1741 gatattacta tcattctgta ctatatgatt caacaggaag ttatataata gatggcagac
     1801 ttggtgaagc tcttagtcat ggatgtataa gattaagtac ggaaaatgca aagtggatat
     1861 atgataatat accagataca acaacagtta taattcatta agaaaaattt tgagtttttt
     1921 attgggataa tgattcttaa attaatagga ttatatatta acttctcata aagagtacta
     1981 tttaaaaagt gtaataagag agtgctatta acaaaacaaa gatattttga ggcactctct
     2041 tataaatata cataattaag atattttcat aaacaattct ataatcttat ctttatcaaa
     2101 gaaaacagga ttagcaccag cacaagcatc tttcatagag ttttctgcaa gcaattcaaa
     2161 attaggatta tcaacaccta catcttttaa agattttggg attccaacct cttcagaaag
     2221 agcttttatc ttatctatta caaagtcaac acactcttta tctgatttat tatttacatc
     2281 cattccaata acttcagcta ttggtcggaa tttggcaggg gctgatttag cattttcctc
     2341 ctcaactatt ggaagtagca tggcattaca tactccatga ggtaaatcat ataaacctcc
     2401 taattgatga gccatagcat gtacatttcc aagacctgca ttactaaagg caattccatt
     2461 taaaaaacaa gcataacaca tttgttctct agcttctatg tcattaccat ttttaacagc
     2521 tcttggaagg tatttaaata tctgctttat agcatataag gcagttgaat ctgtaacatc
     2581 ataagcacca ttagctacta cagcttctat agcatgagtt agtgcatcca taccagttgc
     2641 tgctgttaaa ttagatggtt tgctaatcat taattctggg tcatttactg tcattgtggc
     2701 tagtgaattg gtatcaacca ttatcatctt tacatgtctg tcttcatcag ttataacata
     2761 gtttattgta acttcagcag atgtaccagc agtagtagtt atagctatga taggaagaca
     2821 ttttttagaa gttttgttga taccttcata gtctctaata tctcctccat tagtggctaa
     2881 tatagagata gctttaccac agtcttgtgg agaaccacct cctatagtta taacgaagtc
     2941 gcaattttct gattttaata ttttaagtcc tttattcaca ttagttacag ttggattttg
     3001 tttaacttca tcataaacaa caaagtctat atctatctga gataaaatat ctgttacttt
     3061 ttttacagta ccatttgaaa ctagaaattt atcactaaca ataaatgctt ttttagttcc
     3121 taatgattta attgaatctt tagcttcatt aagacatcct ttcccaagta aatttattgg
     3181 tggtacataa taaatgctca taactaaaat ctccttttat tttcattgat ttttatgtaa
     3241 aaatatatat ctaacaattt tcactaattt actcataata tatcgtatct tagattgaga
     3301 tagggttgct ggttcttata taagaactta attcgtaatt acgattatga ttagaattta
     3361 acatagtatt tttatcatgt caataaaaat ataaaaaata ttataaaaaa aacaaagtta
     3421 ctgtagttaa atcagcaact ttgtttttat agtataaatc aaataaatac attagtttaa
     3481 aacaaaattt ttaattatta tgttttgata tcttccatat acagaatatt aatctaattt
     3541 attacaatgt cttcattacg ttcttccatc attgattgaa tatttttctg agcatttaag
     3601 agtaattttt ttatcagttc tattttttcg tcattagctc taaataaagg cagtgttaca
     3661 ctaacagcag ctcttataca tccatttttt cttattggta cggcaagaca ttttaggtgt
     3721 tccattgatt cctcacactc tgaagctatt tgtgttcttt gaacttcaag aagctggtcg
     3781 tataaaacat caaaatctgt aattgatttt tctgtaaaac cttttagacc atctggatat
     3841 aagcttatta gtttttcctt gtcaaaatca cataaaagag cttttccaag acctgtacaa
     3901 ttagcaggta ttttttttcc aacataagaa ataagtctaa tcggttctgg agaatctact
     3961 tttccaatat aaagaacttc attattgtca agtataccaa gctgacaagt ttcagaacag
     4021 gaattaacta tattagtcat ttcgtcaatt atatagttaa aaatagttct tttttcaaaa
     4081 aatgaagacc ctaatgtgta agaacgtatc cctatagaat atttggatgt gtttttatca
     4141 atagtaatgt attttctagc attaagtgta tgaactatag gaaagatgct actttttgga
     4201 gcattgataa catctgctat ttctgtaaga gtatatccat tttcggattc tgctaatgct
     4261 tctaaaatat ctaatatcct agctgttggg cgatgattat ctgaagtcat aaatttcccc
     4321 ctctttttat taatttaaag tattagaata tgttgatttt agcacactat acgatatttt
     4381 acaatgtaga aaataaatat gtaaatttat atataaataa gctcttgatt tagatacaga
     4441 tagatatttt tttagactgt acattaagga ataactaatt tccaaaatat acctaaagaa
     4501 agtaagtgat atttagttat ataatttaag taaatatatg c
//
LOCUS       DAEMGG010000200         4486 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEMGG010000200 DAEMGG010000000
VERSION     DAEMGG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.201"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(411..1568)
                     /locus_tag="KPK24_003621"
     CDS             complement(411..1568)
                     /locus_tag="KPK24_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG2301251.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            1722..2291
                     /locus_tag="KPK24_003622"
     CDS             1722..2291
                     /locus_tag="KPK24_003622"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG2301252.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            complement(2473..2889)
                     /locus_tag="KPK24_003623"
     CDS             complement(2473..2889)
                     /locus_tag="KPK24_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301253.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(3115..4209)
                     /locus_tag="KPK24_003624"
     CDS             complement(3115..4209)
                     /locus_tag="KPK24_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301254.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
BASE COUNT         1559 a          770 c          478 g         1679 t
ORIGIN      
        1 tcaaaatgat atataaattt atatttaaat gtgtttagag tttaatttat tttcaatgtt
       61 taaaaaagtt attctatgca tctaatttaa ttagtaaact gaggttctag ttagataaat
      121 tatattataa tgaatatata atatgcagtt tacgatattt taaaagtaaa tgatataata
      181 tttggaacaa ctgggttgca atgtgttttt aaaaaatatc aaaataaggc tatgattttt
      241 aaaaccatag ccctgtctgt aaaaatttaa ttatcactta aaaaataaat ttttatttaa
      301 agaatacaca taaatatttc cagactccct catgaactaa aaagttcatt ttgagacatc
      361 tcttttataa tgttctttat tttatagtat aagtttataa aaattaaatc ctatttatca
      421 atctgttctc taagccactt agttgctaca tctaactgtt tattttcatc tttttcttca
      481 atagtaggct ttataccttt ttcattgata taatctccat taggtgtaaa atattgagca
      541 gtagttaact tgtatcctgt tccatccttc aatcttacaa ctgattgtac caacccttta
      601 ccaaaagttg tagtccctac agaaatacct tctttattat caacgattgc agctgttaat
      661 atttcagcag cagaagcact ttctccatta gttaacacta ctattggaag tcccaatttt
      721 tctttatcag atttcaagta ctcagtattt cccttattat cttttgtata tactatggtt
      781 ccttcaccaa ttaactcatc agcaacttct ttacatatat ctagcattcc accaggatta
      841 tttctaaggt ctattactaa tcccttgata ttttttgctt ttaattcttt taaagcttta
      901 tcaaagtcag tatatgtact tgatataaat tgggtaattt ctatatatcc taaatcatta
      961 tcaattactt tactttctat actcttctca attatctggt ctgtttttac tttaaagttc
     1021 aattgtttat cttctcttaa tatagtcagt tcaactgttt tacctttttt acccttcatc
     1081 atacgaacag cttcatctga atttttatat gagacagatt ttccattgac ttttactaat
     1141 ttatcccctg attttacacc tgctttctct gctggagaat ctttcatggg aactactact
     1201 aagccaccat cttttgatgc ccctatatac atacctacac caacataaga accttcacta
     1261 tcattaatta gattctccat ttcatcttta gtataataac cagaatactt atcatttgtt
     1321 cctagaaata gaccttttgc tgctccattt accaaatcag tatctgatac ttttttataa
     1381 aaatcttgtt ttataataga ttctagtcct aacaacttat catatttttt atagtcttca
     1441 tagagttctt tcgatattac aactttattc cccaatgtca actgaaatgc tgatgtaacc
     1501 atagctgtta taattactaa aatcacccct aaaaaaatag ctttcttctt tgaaatcaca
     1561 agatacatct ccttttacat aaactattat tattatatga ctagctgtta tttttattac
     1621 atttacccaa atatattgct ttattacaat aaattatact aaaatttata ttaaatacaa
     1681 ttgctgtaca cattataaca tattaaggga ggaattattt tatgaaaaaa gaatttagaa
     1741 aaaaagttat tgaatcaaga aaaaataaga gtgcaaactt tgtacttagt aattcaaatt
     1801 taataactga aaaattactt caaatggaag atataagaaa tgcaactaat ataatgcttt
     1861 atctaaattt taacaatgaa gttcaaacag ataatttaat caaaaaactt ttgtctctaa
     1921 agaaaatagt atcaagtccc atcacaataa aagaaaatca tacattaatc cctactcaaa
     1981 taacagattt aaaagaggga ttgagaattg gagcatatgg aataagagaa cctaacgaaa
     2041 aatcacctac aattgatatt aaggccttag atgttgtaat tgtaccagct gtagcatatg
     2101 atatacattg ctatagatta ggatatggtg gaggatttta tgatagattt ttagaaaact
     2161 taagaaaaga tgctataact atcggcatag catttgactt ccagatattc gattctattc
     2221 caaaagaaac tcatgatgca caattagact acatagttac tgaaactaga attatcactc
     2281 caaatagtta gaatattaag tatttccaga ccccatttaa tgtagtaata aatactactg
     2341 ttaatatctt actttataag cattttaagt catggctttt acaacaataa gaaccataca
     2401 caatttactt acattaataa aaaccatgac tttattgtac aatatgcagt tatttaatta
     2461 taaaatacct tcttatttcg tcgcatttaa ttctattcta tctggaagtt cactcatatt
     2521 tgtttcaaca actatgtatg gaaaagaatc ttccttatct ttatacttta tcttgactct
     2581 caaaacatcc tttccttcct caaccacact ttctattttg tccatttcta taccatattt
     2641 attatttcct ctagtcacta taatgtatat tttgttatcg attttagccg ctaatgctct
     2701 ttcttcattt gtatacttat ccatcacatc taatatcttt tctggtattg aatttctttg
     2761 taatactgta taatctatgg gtttagattt ctctacaaac atacttggta tgaatataaa
     2821 tccccctata acgaatacaa gagctataac tccataaatt atacctttaa tattaaactt
     2881 tacattcaat cttatcactc ctcccatata ttctaaatat atgatttata aacttatctt
     2941 agactctaaa ttattgatta gtatatattt atacataatt ataaatatat gtaatgtaac
     3001 taaaaaacat gttttttcta tttcataata aacgagctaa aaaataaaaa gagctatatg
     3061 aaaacatttt cattgttttc atatagctct tgagtttaca atattaaaaa atacttattt
     3121 gtcactgatt ccttcttgat atccagatgt ttttgtaatc tgtctataaa gaacacaaac
     3181 cagtattatg acgcccatat atccagtata tggatataaa ataccaacca gcttatcaaa
     3241 tggtaggaag ccacctataa gacctaatat agctgctatt aaagttatta atttaaactt
     3301 aggatgttta tcatctacaa atctattagt tactgaccat aataaaggta cagctgttgt
     3361 atatatacct aatataagaa ttattgagaa taaaactcca attactggtg aaatcttatc
     3421 tgctaagaat aatgatggta ttgcttttga atataagttt cctatatcag aaagaatagc
     3481 taagtgcatc attatagctg cagccataag tgctactcca cctaatatac caccacaaat
     3541 agcttctttt ttgttagcag cacttgctcc taatccagtt aagaatgcca taacaacaat
     3601 tgtgttataa gtagtatata ttacaccaga tagataggca tttggaactg gtttagctat
     3661 ctttacagtg ctaagaactt gtgcagcttc tcctatatta cctatgtttt tagctaaact
     3721 tataccacca actaaaagtg taaagattat tattataggc cctattccac ctagtatctt
     3781 tgataattta tcaagaccta gtgaaacagt taataatgct actactgcca tacctactct
     3841 acctacaaat ggatttaatc catagtattc agttaaagtt gctcctgctc ctgaaatcat
     3901 tactacgaaa acaccaaata agaataaagg tccaaaccat tcaaagaata ttcctaaata
     3961 cttaccacaa taaacacgat aaattttgat aggttcttta agttgaagct ctttaccttt
     4021 tgacataact gaagcaccca tccatgagaa taataccatt gaaattatcc caccaataat
     4081 acctgcaaaa ccataaaaag agaagaattg cattatttcc tgtcctgttg caaatccaga
     4141 accgattact gttgctacat acgcacctgc gaaactgatg actgttttaa catttacttt
     4201 ttctgacata tctcagttac ctccccatgt gaaatttttt cttctgatta aacaattcga
     4261 taaactacct acaatttcct ttaacataaa atacatgata actgttttat agctttttag
     4321 tataataact taaaacttat aatctgattt tataccctac tcctcctctc ataatttttt
     4381 tagattcttt tatctaagta gtcattatat ataaaattag tttctctata aattatatat
     4441 tagtcatatt tttctgaata gtcaatatat atttgaatat tttctc
//
LOCUS       DAEMGG010000201         4385 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEMGG010000201 DAEMGG010000000
VERSION     DAEMGG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.202"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..606
                     /locus_tag="KPK24_003625"
     CDS             <1..606
                     /locus_tag="KPK24_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301255.1"
                     /translation="FIFTQLARVFINKKKINQKIKSRNGFRYDRDFIEARREEIHIKD
                     NNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKNGFGIYIFSGKEKYEGLWKDDK
                     MHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDIYKGYFLDNKKNGKGVLYKNDG
                     NKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNNKLKV"
     gene            765..1271
                     /locus_tag="KPK24_003626"
     CDS             765..1271
                     /locus_tag="KPK24_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG2301256.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1399..2415
                     /locus_tag="KPK24_003627"
     CDS             1399..2415
                     /locus_tag="KPK24_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG2301257.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2769..3473
                     /locus_tag="KPK24_003628"
     CDS             2769..3473
                     /locus_tag="KPK24_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG2301258.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            3768..4247
                     /locus_tag="KPK24_003629"
     CDS             3768..4247
                     /locus_tag="KPK24_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG2301259.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         1850 a          369 c          752 g         1414 t
ORIGIN      
        1 tttatattta ctcaactagc tagagtattt attaataaga agaagattaa tcaaaaaatt
       61 aaatcaagaa acggatttag gtatgataga gattttattg aagccagaag agaagaaata
      121 catataaagg ataacaataa taataaaaat aagagtaaca ataagaaatt aaaggaagaa
      181 aaagtattta aatacgataa tggtgattta tataaaggtg agtttgttga tggaaagaaa
      241 aatggatttg gaatatatat tttttcaggt aaggaaaagt atgaaggatt atggaaagat
      301 gacaagatgc atggaatagg aaaatatact tatagagatg gttcaatata tacaggtgaa
      361 tttaaatacg gattaaaaaa tggtttggga aaattaacat atcccaataa tgacatttat
      421 aagggatatt ttttagataa taagaaaaat ggtaagggtg tattatataa aaatgatgga
      481 aataaacaag caggaatatg ggaagatgat gaacaatgta aaagtttaga ttttaaagat
      541 ttaaataata ataaaataaa taatgtttat caaaatagat taaataacaa taaattaaaa
      601 gtttaattat tctaataaaa aaatctttat ttatgatgga atataggata tatctatgtt
      661 gtaatttgta aaatcttcct atatcatata aaattaagta ttgattagtc tattacataa
      721 atatagaata taaatatatc catttaataa taaggagaag agatatgaaa gagtatgtag
      781 ttgtggattt agaaactaca ggattagatc catataaagg ttgtgaaata atagaaatag
      841 gaataacaga aataaaaaat gaacaaattg taaaaaacta ttcaaggttg ataaaaccta
      901 aggggataat ttcttcattt ataacagaat tgacacatat aagtaatgaa atggtagaaa
      961 atgaagaacc actagaatta gttttaccta gatttagaaa atacataggt gatagaacta
     1021 taattgcaca caatgcgaaa tttgatttaa aatttttaaa ttactatctt agaatgttag
     1081 atttagaacc aataaacaat tatatatgta cagtagagtt attaaaaaaa tgtaaaagtt
     1141 ataaaggaaa aaacaagaaa cttgaaacag cttgtgctta ttacaacata gagaatataa
     1201 atgctcatag agctgacagt gatacattag caactgcaaa actatttttg aaaataaaag
     1261 atgaatatta attataaata tagagaaaat tagtgaaaat atcctttata aaattgaccc
     1321 ttaagcttac aattttgttc tttgatttat tttgtagaac aatgtataat gtaccttata
     1381 tgaagaaggg agactaaaat gggttattac aatgataatg ataataataa aataagacgt
     1441 aaaaaaatta gtaacaacaa agatagtaat aaaaaatcta aacattctta tacttcggca
     1501 aataaaaaaa ataaaagtcc taatacaagt tccagtaaaa gaatgagtga aaaagtagat
     1561 tcacagaatc ttataaaaag agatagagat agttttacaa gagataatgt aagaccttta
     1621 aatacagatt acaatcaaag agaaagaaga agagtcgagt ataaacaaag agagaaaaaa
     1681 ttaaaaagta aaaaaagaaa aaaacaaaaa aggattagaa tagttagggc attattatct
     1741 attatgttag ttgcactaat tatattttca gtggcaggtg caatatttac agtttcagct
     1801 ataaaagggt caccagaagt tactaaaaag ttaattaggt ctaaatatat aagtagtgag
     1861 gttgtaagta taaaacaaat gccaaatgat ttaaaacatg caatagtttc catagaagat
     1921 gagagatttt ataagcataa aggagtagat gttatttctc tagttaggtc agtattaaac
     1981 aatgtgttta cagatacaac acaaggtgca agtactatag atatgcaagt ttcaaaaaac
     2041 ctgcttacta gtaatgataa aactatgaag cggaaaataa gggatatgta taatgctaag
     2101 cagatgaata agattatgac taaggatgaa attcttgaag catatttaaa taatatgtat
     2161 ctaggtaaaa atgcatatgg agctgcaaag ggagctgaag tctactttgg taaaaaagtt
     2221 agtgaattaa atttagctga gtgtgctatg ttagctggta tgacaaataa tcctgcaagg
     2281 tatattgacc atggagaggc taagaagaga caagtgattg tactgtataa gatgcatcag
     2341 cttggttata taactgataa ggagtataga atagcaaaag ctgatgacac tccatttaaa
     2401 tcagaaattg attaaaattg taagaatata gtattttcaa tagtttaaac aaacaaaaat
     2461 gtaataaaaa aggtgatttg ccacccattt gccaccataa ttataatcta atggcaaatt
     2521 gttttaatgt acaaactaga taatagaata ttgtcttaaa tgctatatta tgctaaaatt
     2581 taattgtatt ttgtaaatta aaaaacttat aatttagttt tctagggttc cgcattttat
     2641 aatggtcagg tccaagagaa aacgcgcaat tgttgtgtac acggaaggat aaaagcctgg
     2701 gagatattta agaatatttc tataggcttt tttattttta catattttaa atttgaggag
     2761 gttttctaat gaaaaaattt gttattgagg atgcattttg gaatctattt cctagtgcaa
     2821 aaattggagt tgttatttgt aatgatatag ataactctat aaaagatgaa gactataaga
     2881 aaataatttt gaagggagaa gaagaagcat taaaatactt ggaggactca gaatttagca
     2941 acaaccaagt gattaaagta tggagagaag catttaaaaa atttaaaacg aaaaaaggag
     3001 caaggtcttc tattgaagca ttattaaaaa gagttcataa tggaaataat ataggaacta
     3061 ttaacccttt agtggatatc tacaatttta tctcattaaa atatgcatta ccttgtggag
     3121 gggaggatat agataaattt ataggagata ttaggctaac tagagcaatt ggtaatgagg
     3181 agtttatccc actagggaca gatgaaaatt catttccata tgaaggagaa ataatttata
     3241 aagatgatgg tggagcaatt tgtagatgtt ggaattggcg tgaagcagtg agaacaatgc
     3301 ttactgaaga tactaacaat gcctttttat gcattgaatt agtagatgaa agtagattta
     3361 aagaatttga aaatgcttta aatgaattag ctaaaatagt tcaagaaaaa ttgggtggga
     3421 attgtaaaat ttcaatttta gatattgata ataaagaggt atcagtggat taatatggag
     3481 gtgctgttgt aatggactat tctagttata aagatactta tatttagggt aagaaggtct
     3541 attttagata taacgattat caaaggatat aatatagaaa ataaaaatgt agaatataaa
     3601 aagcctttaa aataagaaaa aaatgtattt taaaggcttt tattatactc ttttttattt
     3661 gcataattaa caataaaaat atattgttaa ttataatatt tttatttaaa aacgatataa
     3721 tataatgtat tatactatat ataaaatatt ttaaaggagt tggcatgatg tctaataaac
     3781 ttataaaaaa tttactaagt ggtatattac aaattttatt ttttctttta ggattagtaa
     3841 ttgttgtagg aggatttaaa agttttatgt acctctgttt tagtggagaa gcaactctac
     3901 aaggtactat ttatggaata ttaatgttta ttttaggtgt atcatatttt atcattataa
     3961 aatcattgat agaagtatta ggtagttctg aatatagcct atttgtaaaa gagaatgtaa
     4021 aaagatttag gattattgga tatcttttgc ttttaaactc tctaattgag tttatttcaa
     4081 cttttggtac tactggaaaa ggaatgagat ttctagattt aggatttgga ttttatttta
     4141 ctgttccagt atttgtatat tttataactt ctctgatgag ttttgtaatt gcagatggat
     4201 ttgtaaaggc aataaagata aaagaggata atgatttaac tatttaggtg tgacacttta
     4261 atatatagga gataaataac ccgaaaagag tgactaacta gtaatttaaa ataaaatata
     4321 ttagggtata atacataaac tataatgcta aaattaaaag attataatac taaaatataa
     4381 ttatg
//
LOCUS       DAEMGG010000202         4357 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEMGG010000202 DAEMGG010000000
VERSION     DAEMGG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.203"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            190..1788
                     /locus_tag="KPK24_003630"
     CDS             190..1788
                     /locus_tag="KPK24_003630"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG2301260.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(2023..4104)
                     /gene="bclA1"
                     /locus_tag="KPK24_003631"
     CDS             complement(2023..4104)
                     /gene="bclA1"
                     /locus_tag="KPK24_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBG2301261.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGVTGEIGPTGVTGPTGVTGSIGPTGATGPTGATGVTGEIGPTGATGPTGNT
                     GVTGATGPTGNTGVTGVTGATGPTGATGVTGEIGPTGATGPTGEIGPTGATGPTGATG
                     VTGEIGPTGVTGPTGEIGPTGATGPTGVTGSIGPTGATGVTGEIGPTGPTGVTGSIGP
                     TGATGPTGNTGVTGVTGPTGATGPTGVTGEIGPTGATGPTGVTGEIGPTGATGPTGPT
                     GNTGATGSIGPTGVTGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNN
                     GDAITGWQTIIGNSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFP
                     ILGGKDLAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATL
                     TIFRIADTVMT"
BASE COUNT         1276 a          782 c          678 g         1621 t
ORIGIN      
        1 atatttgatt gggtaaaagt atatataata ataattttta tattaaattt gtagtattta
       61 tgtaagaaat aaattttata taatattttt tcactaaatt tttttctttt tacaacaaat
      121 actcactacc ttttgatata atatttttat caattattat tatattcaag ttttgaaagg
      181 agtattaaaa tgagtttatt agttttcgga cataaaaatc cagatacaga ttcaatatgt
      241 tctgcaattt cattagcaca tttaaagaac aaactaggaa taaaagcaac agcttatgct
      301 ttaggagata tcagaaaaga agctaaatat gccttggatt actttaaggt agatgcacct
      361 gaaattttag ataatgttag aatacaagtt agagatttaa attatgataa agtagtacct
      421 ttaacaccaa cttcatctat attagaggct tataacctta tggatgaaaa aaatgttaaa
      481 acactacctg taacttatga tgatggaact tttgctggta tcattactat gaaagaaata
      541 gctaaaaatc tacttcatca acactttaca actatacaca catcactatc aaacatttgt
      601 aaaaacctta atggtactat tcttgtggac tgtggtgaag atataaaagg tagagtagtc
      661 actttatcat ttggaatgga gactctaata gagactttaa aagaaggaga catagctatt
      721 gttggagata gatatgatag tattgaatat gctattgata caaaagttaa acttttaata
      781 cttactaatc atacagagat ttcaaaagat ttgctagctc ttgctaagat aaatggagtg
      841 tctgttgttt ctgttgaaag tgacaattat aaaacttcta atgtaataaa tcaatgtagt
      901 ttcttagact ctatagaagc tacagaaaat ttaattacat tcaaagaaga tgattatatg
      961 gatgaaataa aagagattat gctagaaaca aacttcagat cttatccaat attagatgat
     1021 gataataagt ttttaggttt agtttcaaaa ggtcatctat taaacccatc taaaaagaat
     1081 gttgttttag ttgaccataa cgaatatgct caaagtgctg atggaattga acaagcaaac
     1141 atagttgaaa taattgacca tcataaactt ggcggtattt ctactgatgt tcctatgtca
     1201 tttagagtta tgcctgttgg atgcaatagt actataattt atcaaatgta caaagaaaat
     1261 aatgttgaga taccttatga aatagctggg cttcttttat ctgctatatt atcagataca
     1321 ttattattta agtctccaac aacaactgat atggataaga aagcttgtga agaattaagt
     1381 aaaatagcaa aagtagacat ggaaaaatac gctatggata tgtttaaatg cggtacttct
     1441 ttagatgaat atactataga agaaatagta aatatggact ttaaagaatt taatatgagt
     1501 ggacatagag ttggtattgg acaagtattt accttagaca tagactctat atttgctaaa
     1561 aaagatgagt ttttatctta tataaattcg actgattatg ataaattaat tctttcagtt
     1621 acagatataa tcaaagaagg ttcttattta atctataaag cagaagatag cttaattgct
     1681 aacgcatttg gtgttgaagg tgttcaaggc acatttgcac caggtgttgt atcaagaaaa
     1741 aaacaattag tccctaattt aactactgca atcaaaaatt ttaaataata taaaattttt
     1801 aaataacgaa aactgtctca aatctatatt tgagacagtt ttttattata tatatattga
     1861 taataaagtt aaataaaaat ccataaattt aaaattttta tgaatatatt ttattattat
     1921 tgttatttta ttcattctaa aataggtaaa actcaatata taaaaatact tttgtcattt
     1981 aaataaaacc taaatagttt ttaagattat tttagacacg ttttaagtca taacagtatc
     2041 agctattcta aatatcgtca aagtagccgc agttcctgct gattgcccat ttcgtatccc
     2101 tactgttgta gatgaaaagt tatttattgt aaaagtagtc ccaccaaata aaaaaccagc
     2161 aaaatatcca gaaagtactc ctccgcctcc tggctcagta gtaagccctg ccaaatcttt
     2221 tcctcctaaa attgggaata ggatagcgaa agaatattga tttatacttg atgaacctgg
     2281 ttgtaatgct actgaaactg atatataata cacaccattt tcttgtactg taaacgtacc
     2341 atttgcatct actgttatac ttgaactatt tcctattatt gtttgccaac ctgttatagc
     2401 atctccatta tttagtgttt gtggagatgg tgcatttacg aggaagttag caactggctg
     2461 agaggaattg cctgttgctc ctgttggacc tgttgggcct gttactcctg tcgctcctgt
     2521 tgctcctgtt ggacctgtta ctcccgttgg ccctatactt cctgtcgctc ctgtatttcc
     2581 tgttgggcct gttggtcctg ttgctcccgt tggtcctatt tctcctgtta ctcctgttgg
     2641 gcctgttgct cccgttggtc ctatttctcc tgtcactcct gttgggcctg ttgctcccgt
     2701 tgggcctgtt actcctgtca ctcctgtatt tcctgtcggt cctgttgctc ccgttggtcc
     2761 tatacttcct gtcactcctg ttgggcctgt tggtcctatt tctcctgtca ctcctgtcgc
     2821 tcctgttggt cctatacttc ctgtcactcc tgttggtcct gttgctcctg ttggtcctat
     2881 ttctcctgtt gggcctgtta ctcctgttgg ccctatttct cctgtcactc ctgtcgctcc
     2941 tgttgggcct gttgctcccg ttggtcctat ttctcctgtt gggcctgttg ctcctgttgg
     3001 ccctatttct cctgtcactc ctgtcgctcc tgttggacct gttgctcctg ttactcctgt
     3061 tactcctgta tttcctgttg gacctgttgc tcctgttact cctgtatttc ctgttggacc
     3121 tgttgctccc gttggtccta tttctcctgt cactcctgtc gctcctgttg gacctgttgc
     3181 tcctgttggt cctatacttc ctgtcactcc tgttgggcct gttactcccg ttgggcctat
     3241 ttctcctgtc actcctgttg ggcctgttgc tcccgttggg cctatacttc ctgtcactcc
     3301 cgtatttcct gtcggacctg ttactcctgt tggtcctata cttcctgtcg ctcctgtact
     3361 tccggttggg cctgttactc ccgttggccc tatacttcct gttgctcctg tatttccggt
     3421 tgggcctgtt ggtcctatgc ttcctgtcgc tcctgtactt ccggttgggc ctgttactcc
     3481 tgttgggcct gtacttcctg tcgctcctct tggtcccatt ggacctgtaa ttctaattgg
     3541 aagacactca catttaggat aacaattatc tataattcta tcttcaaatg ttacaagtat
     3601 atacggtttg ttacaacctc tactagatgt aaattcaaga atctgataag ggtaatttaa
     3661 agataaagct aacccataat taggatattt attcattgcc catgctttta tagttcccgt
     3721 aatatttaaa caaatatagt tggatgtatc acttatccca attggtaaat atcccctgat
     3781 attttccata gacactctat cattccatgt tatagttcgt aaattataat attcacttat
     3841 tcttttaatt tcaatatcta ctttttcttt aaaaatatga ttcttatttg aatcaatatg
     3901 aataaataac tctgctttta atatactttt gtcttttaat atatctggta ttctcacttt
     3961 aaaaaaagta atcaactttt cttttacaaa actatttgaa catttacttc ctattaagca
     4021 ataatctaaa ttactaaagt ttttatctgg atatttttta gatataaaag tatcatcatt
     4081 taaataaagt ataatatttc tcatttataa tccctccatt ttgggtctat atcatactat
     4141 gtttaaaata catgcttagt tacaatacaa ttaaaattta tagtattaaa tttttatata
     4201 ataatgctga tttctacaaa aaaagaacat gtcattagaa atttctaata gcatgttctt
     4261 tttcattatt atatattaca ctttaaatta ttccttgact ggcttagctg gtgtaaacta
     4321 aaccagtctt tctaaaatat taagcacttt tttctta
//
LOCUS       DAEMGG010000203         4349 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEMGG010000203 DAEMGG010000000
VERSION     DAEMGG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.204"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            334..999
                     /locus_tag="KPK24_003632"
     CDS             334..999
                     /locus_tag="KPK24_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG2301262.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            1021..1953
                     /locus_tag="KPK24_003633"
     CDS             1021..1953
                     /locus_tag="KPK24_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG2301263.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            1974..2615
                     /gene="pcp"
                     /locus_tag="KPK24_003634"
     CDS             1974..2615
                     /gene="pcp"
                     /locus_tag="KPK24_003634"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG2301264.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2735..3067)
                     /locus_tag="KPK24_003635"
     CDS             complement(2735..3067)
                     /locus_tag="KPK24_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301265.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            3333..3953
                     /locus_tag="KPK24_003636"
     CDS             3333..3953
                     /locus_tag="KPK24_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2301266.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNKLIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
BASE COUNT         1645 a          582 c          644 g         1478 t
ORIGIN      
        1 ttatcatttc aaaagtgaaa aatacacttg tctctatgtt tggcacattc tctttttata
       61 atataatatc taatttaaaa cctatttcaa atattgacat atacttaaac tatagactta
      121 caaaatcaat tcatattagc ttaaaatata atctttgtta ttcaaattcc tatacacaac
      181 cattatttac tttgatactt taaaacatct tccctatttt ttattaaatt ttataataat
      241 agtcctatta acgcaaatac tataaaaaga gtttaacttt atccattaaa taaatttaaa
      301 ttaaaacata tagaaatgga aggagaacaa tttatgataa aactaatcgg tatattgata
      361 gttgtaattg gatttatact taaaattgac acattattta ctgtattatt agcaggagtg
      421 gcaacaggaa ttgttgcagg attagatttt aatcaaattt ttacaatttt aggagattct
      481 tttgtctcaa acagaggtgt atcattattc atattaacat tgccagtaat cggtgtatta
      541 gaaagatatg gtttaaaaca aagagctgtt tctttaatag aaaaattaaa aagacttaca
      601 acaggaaaag tacttactat atacatgata gctagacaaa tagctggtgc tctatcaata
      661 agaatgagtg gacatcctca atttgtaaga ccacttgtta atcctatggc acaagctgct
      721 ggtttaagta atagtgatga actaaaagaa tcggatgaag aagccataaa ggcattatct
      781 gctgcatcag aaaactatgg aaatttttat ggtcaaaact tatttgcagg tagtagtgga
      841 gtattactta tagcatctac tctgactcaa tttggataca atgtaactgg acttaatatc
      901 gtaaaggcta gtactattat ggcagtaata gcacttgtag tggctacaat ccagtttacc
      961 ttatatgata aaaaattaaa caaaagtcat aaaaaatagt tttatttagg aggaataaaa
     1021 atgcaaaatt ttataaacat atcattagaa atcttttacg cactaatggg atttttaatg
     1081 attgttatag catataagtc ttttactacc ataaataaca ataagaaata tggtacatca
     1141 ttattttgga tacttatatc actgccattc atatttggta gattgattcc agccaatata
     1201 ataggaataa tattaatatt atctagttta ttaacacttt ctaaacaagt tgtatttgca
     1261 aagtatgaag aaccagatga aaattttgga aaagaacaag cagacaaact taaaaataaa
     1321 atatttatac catcattaat attagcattt gctgcagtag tagtagctat gagcctttct
     1381 aattttgata attcttctca atttgcaata ggtgtgggtt caataattgc attaatatca
     1441 gcccttataa taactaaggc aaaacctgct acatcagttc aagatggctc aagattactt
     1501 caacaaatgg gacctgctag tatgttacca caacttttag ttgcattggg agctttattt
     1561 actcaagccg gagtcggtga agttatttca accatgatat ctggtgtagt ccctgctgat
     1621 agcagattat ttggtgttat agcttatgta ttaggtatgg ttatatttac catgataatg
     1681 ggaaatgcat ttgccgcatt ttcagtcata acggcaggaa ttggtattcc ttttgtattg
     1741 tctcaaggtg gaaatcctgc tataattgga gcattagcat taacagcagg atattgtgga
     1801 actctactta ctccaatggc tgctaatttt aatatagttc cagcagcctt gctagaatgt
     1861 aaaaatgatt atattgttat aaaatatcaa gctccagttg ctttggtatt aattatagcc
     1921 cacatattag taatgtactt tttaggattt taatataatt aggaggtaaa ataatgaaaa
     1981 ttttgttaac aggatttgac ccatttggtg gagaaccaat aaacccagct caagaagcag
     2041 ttgaaagggt caataataat ataaacggtg cagaaatcat aaagataact ataccaacag
     2101 ttatgacaaa atctgttgaa gctatagata aagctattca agagcacaat ccagatatag
     2161 taatatcagt tggacaagct ggaggtagat ttgacattac acctgagaga gttgctataa
     2221 atatagatga ttttagaata aaagataatg agggtaacca ggttatagat actattataa
     2281 aagaagatgg tgaacctgca tatttttcaa aattgcctgt aaaagctatg gtaaaacata
     2341 tgaatgaaaa taaaattcca gctagtgttt caaacactgc aggaacattt gtttgcaatc
     2401 atgtgatgta tggaattctt tatatgatag acaagaaata tcctaatata aggggtggat
     2461 ttatacacat cccatataca acttcacaag taatagataa aaaaaataca ccattcatgt
     2521 ctttagaaga aatagtaaaa ggcttagaat tagctataga agcttgtatt atatataaag
     2581 aagatgtaaa agaaattggt ggtgaaattt cataataaat tttttagtat ttttataaat
     2641 taaaaatact aactattaat ttaaatatag actgtgagta gattattcaa acaattttac
     2701 tcacagtctt tttatggtat ctacagatac aaaattaagg tctattcctc tctccccact
     2761 cacacattgc atctaaaatt ggaatcagag attttcccct ttctgataga ctatattcta
     2821 ccttaggagg tatttgagga tattcagtac gaataatcaa gttatctgct tctaattctt
     2881 ttaatgataa actcaaagtt ttataggaga tagtatttat ataacgtttt aatgcattat
     2941 atcttacaac ttcaaattct acaagacagt aaagaattat catcttatat ttaccactaa
     3001 tcagtgaaag tgtataacta aatcctgttt cacttaaatc tgtatttgat atacagtcta
     3061 tactcatata ttacactctc cttttggtta gtattgaaca aaaatgtgcg tacttgtttt
     3121 tagaataatt tataataaaa ttatagtgta agaataattg aaagtcaatt ttataatagg
     3181 aggtgtattt actaaaaaaa aagttatttt aaataggatt ctaagattag acatttctat
     3241 ataaattgat aatattcatg aaaatcctat tttataaaaa ataatatctc ttagaaaatc
     3301 tattaaataa aattcattgg aggaaattta taatgaaagt tttattgata aacggtagcc
     3361 caaaccaata tggatgtact tatacagcac taaatgagat tactaaagta ttgagtaaac
     3421 acgatattaa aacagaaata ctgtatttag gtaaagaaac aatcccagga tgtattagtt
     3481 gcgcatcctg ttttgaaact ggaaaatgta ttcgaaatga taaagttaat aaattgattg
     3541 aagatctgga taatattgat ggtattatta ttggttctcc tgtttacttt tctagtgcaa
     3601 ctggtcaatt aacttctttt cttgaccgtc tatttttcat agctggaagt cgcatggcta
     3661 caaaattagg tgcttcagtt gtgtcatgta gacgtggtgg tgcaagtgca acatttgacc
     3721 aactaaataa atatttttca atcagcaata tgcctattgt atcatcacag tattggaacc
     3781 aggtacatgg ctttactcca gaggatgtta tgaaggatga agaaggatta cagactatgc
     3841 gaactctagg agaaaacatg gcatggcttc ttaagtgtat atcagcaggt aaaaaagctg
     3901 gaattaaaga accacaatat gaagaaacaa ttatgactaa ttttataaaa taaataaaaa
     3961 atgtactaat attaattaaa acaaaacact aatttataaa taaaataagc ataaatgtag
     4021 gataaaaagc actaaaacag aggtattgaa ttttatatca aacctctgtt ttattattac
     4081 attaataacc aaaccatgta taacatcata tatttctaaa taaattataa tattatattc
     4141 taatgttatt gaaattacaa taactgaatt tctgctcata aatttaaata ttctacaaat
     4201 ctcatctaaa taacaatttt aatagaccta aaacaatcct catactgttc attttgtatt
     4261 aaaaatatca cttacaatca aaaaccattt attaagtata ttcctaagat atacaatata
     4321 tatacatgga ttggatagta taaataaaa
//
LOCUS       DAEMGG010000204         4299 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEMGG010000204 DAEMGG010000000
VERSION     DAEMGG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.205"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            557..1480
                     /locus_tag="KPK24_003637"
     CDS             557..1480
                     /locus_tag="KPK24_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG2301267.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1455..2282
                     /gene="panB"
                     /locus_tag="KPK24_003638"
     CDS             1455..2282
                     /gene="panB"
                     /locus_tag="KPK24_003638"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG2301268.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGTFPQEKHSFSINESELEKLY"
     gene            2301..3149
                     /locus_tag="KPK24_003639"
     CDS             2301..3149
                     /locus_tag="KPK24_003639"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG2301269.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3560..3742
                     /locus_tag="KPK24_003640"
     CDS             3560..3742
                     /locus_tag="KPK24_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301270.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT         1697 a          501 c          592 g         1509 t
ORIGIN      
        1 taccttaatt taattagaaa gtttattact tcatatttta aattataatt gataaaaaaa
       61 aatacagcaa tatagtatta ccaatttaaa aaaaattaat attacataat acatctctct
      121 cttatattaa attttaactt ggtactaatc ttgacaaatt gtccacaaaa ataaattata
      181 tattttatta ttgttttaaa tttttataaa aaatcagtat tattgatact attgaagtta
      241 aaaagcatta atttctaaaa acatcataat ttttctaata gacttaatca cttttaatat
      301 atatagtaaa acaaaaaact tatattttat aaatatcata atttttttac tgatatttta
      361 ttatataaat tttatagaat taattgtcaa ttttttatga aaatggtaag atatttatgg
      421 atggtatctt gttataagaa ctatacttta aatgttttta attattttgt cttatgtaac
      481 tcatatcctc aggaatgaga ttagaaatta agatgttgta tcacaaagtg gaggtgatac
      541 tattaaaata ggctttattg gttctggaaa agttggaaca tctttggcaa attatttttt
      601 atcaaaaggt tattgtgttt caggctttta tggaaggaat caattatctt tattagaatc
      661 aacaaattta acaaaaacaa agatttacag caatttgaaa gatataattt atgaaaacga
      721 tatattattt attacaacat cagatgattc tattgaaatt atagacaaaa aactttccaa
      781 atttaattta aaacataaat acatttgcca cacaagtggc tctttaaaat ctagtatatt
      841 gttctgttca aaaaaagcag gtgcattaat ttattctatc catcctatat ttgcattttc
      901 caataaaaat accaacataa aaaaaatgaa agacatttgt ttttccatag aaggtgataa
      961 tgaacaagat gatttagtta ttcaaaattt catagatgat ttgggaaacc aattttttat
     1021 aagagataag gatacttcat ccacttatca tttagctaat gttttagttt caaatttagt
     1081 attatcttta ctagatatag gagtaagtta ctttattaaa ttaggtttat ctgaagagga
     1141 atctttaaaa gctatcagtc ctttagtaaa aaaaaatata gaaaatatac ttgataatgg
     1201 ctttactaaa gctcttactg gtcctgtttc tcgtggagat ataacaccaa taagaaaaca
     1261 tctatctgta ttggataaaa aagatgagga tatttacaaa atactatcct taaacctact
     1321 aaaaataatt gccttaggaa atgacaattc attaaaaggt ataaaaaata ttactataga
     1381 aaatgcaatc gaaaatttaa taagtaaatc tgaaaaatac aaagaaattt acaaactttt
     1441 aggaggaaaa aacgatgaaa aataccatac agacttttaa gaatgctaaa tatgaaggaa
     1501 aaaaactatc tatgcttact gcttatgact actcaattgc aaaaataatg gatgagtgtg
     1561 atataaatgg tattttaata ggagattcat taggaatggt tataaaaggt gaagaaaaca
     1621 ctctatctgt taccatagat gaaattatat atcatacaaa agctgtaaaa aatggtgtaa
     1681 aaaatgcctt aatcgtaagt gatatgcctt ttctatctta ccatgtttct atagaagatg
     1741 cagttaaaaa cgcgggtaga cttataaaag aaggaggagc tcatgctgta aagttagaag
     1801 gtggctcaaa cgttataaag caaatagaaa gcattgtaaa tgctcaaata cctgtaatgg
     1861 ggcatttagg attaactcct caatcagtaa attcatttgg tgggtttaag gttcaaggaa
     1921 ataccagtga aacagcaaga caacttattg aagatgctaa attaatagaa aaagcaggtg
     1981 cattctctat tgtccttgaa ggagtgccta cgaaaatagc tgagatggta actaattcta
     2041 tttcaattcc tacaataggt ataggggcag gaattaactg tgatggacaa attttagttt
     2101 atcaagatat gttgggaatg tttggagatt ttgttcctaa gtttgtaaag cagtatgcaa
     2161 atataggaga tataatgaaa gattcaatta aaaattacat cctagaagtt aatacaggta
     2221 catttccaca agaaaaacac agcttttcaa taaatgaatc tgagttagaa aaattatact
     2281 aatattaagg atggtacaaa atgcttgtaa aagaaattaa attactaaga aatataatca
     2341 aagattggag aaaacatggc tattctatag ggcttgttac aacaatggga tttttacatg
     2401 aaggacacca aagtcttata aaaaaagctg taaaagaaaa tgataaagta gtagtgagtg
     2461 tatttgtaaa tccaactcaa tttggaccaa atgaagattt taatagctat ccaagagata
     2521 tagataaaga ttttaaatat tgtatggata gtggagcaac agtagtcttt aacccatcac
     2581 cagaagaaat gtatttaaaa ggtaattgta ctaccattaa tgtttctgga cttacagatt
     2641 ttctttgtgg agctaaaaga cctgttcatt ttggaggtgt ctgcctagtt gtttcaaagt
     2701 ttctaaatat agtaacacct gataaagctt attttggaga gaaggatgcc caacaattag
     2761 ctgtaattaa aagaatggtc aaggatttaa acatagatac agaaataata ggctgtccta
     2821 taattcgtga aaatgatgga ttagcaaaaa gttcgagaaa tacatatctc tctgaggaag
     2881 aacgaaaatc tgctctaata ttaaataaga gcttaagttt agcaaaagaa gaattagtaa
     2941 aaggcaatct aaatccagag aatataaaag aattaataac agctaaaata aattctgaac
     3001 acttagcaaa aatagactat gttgaaattg tagatagtga gacattacaa cctgttaagc
     3061 aaatagaaca ttctatactt gtggcaattg ctgtgtttat aggaaaaacc agactaattg
     3121 ataattttac atttgagtta aatatatagt ctataatttt atataatata tctaaaaaat
     3181 aatagaatac ctaaaaagta ataaattacc taaaaaataa taaattgctt agaaaataat
     3241 agattacaat aactaaaatt ttaaaattaa attccttaaa agtatgatta attataatta
     3301 tataagtata ttcttattac acttatatta atttatatta aatatctact aactcaaata
     3361 ttcataatta aactttaaac tggataaatt ttcttttaag aatgaaagtt tatccagttt
     3421 tgcgattata ataaccagtt ttacatatcc acttgtaaaa tatatctaag caatatatca
     3481 tactatatag atttatgagt gaaataatat ctgctattta ctgaatattt ttatttttcg
     3541 ttttttaggg ggattagttg tgtttaaaga taagattgat gaatgtgttc atataatgac
     3601 tgcatatatt gctagtttaa aagaatacta ttcattcata gaaactcaaa ttggtgattt
     3661 tattaaaaaa tatggagaag atgttgtaga gttatgtctc catagagtga tgattctact
     3721 atgcgaatgt ggtttggcat aaaattaaat ttattaaaat gggtaagaag aatcctatat
     3781 aatattcttc ttacccattt taatatatca atttcatagc ttatcgttta aaatatttta
     3841 catccattac tagacaactt tacattctct ttttaattct agtatattta aatcatttta
     3901 gttgctcctt atttattctt attacttgtt ttattttata aataaaataa ttctttttaa
     3961 taatgttttt tgttgatatt cctatcacac atccaattat ttatcattgt cttgaaattg
     4021 tttgcatact taatttaatc caattgaaac accccagcat agaatcccct gtaaaataga
     4081 agttggatta aatcctaaca tctatacaga aaatactact ccaagcacaa gcaatataat
     4141 ggaatatatt tatcacttaa ttttttatac tttttaaatc ccatacctaa agcattaagt
     4201 gcagctatta gaattaataa ttgttgtggt ataaaattat gcttgcttca tatttattca
     4261 ttttttacta taaaagttct tgttttacaa aaaacattt
//
LOCUS       DAEMGG010000205         4289 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEMGG010000205 DAEMGG010000000
VERSION     DAEMGG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.206"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            231..1310
                     /locus_tag="KPK24_003641"
     CDS             231..1310
                     /locus_tag="KPK24_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG2301271.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="KPK24_003642"
     CDS             1634..2002
                     /locus_tag="KPK24_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG2301272.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDNDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="KPK24_003643"
     CDS             2119..2922
                     /locus_tag="KPK24_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2301273.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="KPK24_003644"
     CDS             3307..4197
                     /locus_tag="KPK24_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2301274.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVFKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1642 a          470 c          803 g         1374 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagaacgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tgctacctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtacgg taaaaataaa tctactaact
      901 ggattgtaaa gcatggttgt agaactctac taaaaaaagg aaatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagct atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttagcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaaag gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggataa tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtttttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgatgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaacaa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaattaca tactcattag
     2941 gtaaattatg tagtatatgt gtttatagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga agtattagaa ttagtggaga taaaacagct attagagatt
     3121 ataaataaat agttttaaag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 aggattataa atgattagtt taaaagtact atcggacagg tctctgcata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagagat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggggta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaggcac tagatttgat ttcggaaaat cattttggag tgtctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgaggcagtg aaagaactta atgattctgg aattttttgt
     3841 ggtatattat tgactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgtatgta tggtgtaaca
     3961 atgcgtagtg gtcagcgaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtatttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tgttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gatattataa aagcgtacaa gaggtcggag tcatatactc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtggtgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtatt
//
LOCUS       DAEMGG010000206         4188 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEMGG010000206 DAEMGG010000000
VERSION     DAEMGG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.207"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            161..1114
                     /locus_tag="KPK24_003645"
     CDS             161..1114
                     /locus_tag="KPK24_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG2301275.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1104..2060
                     /locus_tag="KPK24_003646"
     CDS             1104..2060
                     /locus_tag="KPK24_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG2301276.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            2057..2812
                     /locus_tag="KPK24_003647"
     CDS             2057..2812
                     /locus_tag="KPK24_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2301277.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2904..3869
                     /locus_tag="KPK24_003648"
     CDS             2904..3869
                     /locus_tag="KPK24_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2301278.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1709 a          386 c          683 g         1410 t
ORIGIN      
        1 aaattttaat gttactagaa gtttcaattc tagtaacatt ttttatgtta aaaacagata
       61 tagatatata ttgacaatga taattattat tgataaacta taaaagtgaa aatgataata
      121 attttcattt taattaataa aagattttta aggagggaga atgaagaaaa ggtatttagt
      181 cataggaata ataattttgt cttatttttc tttatttata ggagctgaag atataaatat
      241 aatgcatata tttgccaaaa atcagcataa acttatgata tttatcatga gtagaatacc
      301 aagattaata agtatattga ttgctggtgt aggaatgagc atagcaggtc ttataatgca
      361 acagataagt aagaataaat ttgtatcacc tacaacaggt gctacaatag atgcagcaca
      421 atttgggata gtaatatgta tgcttttagt accaactgcc tctattttta caaagacaat
      481 aatagcattt gtattttctc ttgtagggac ttttacgttt atgaaaataa tagggaagtt
      541 acaatttaaa aatataatct ttgtaccatt agtaggtata atgtttggaa atataatagg
      601 ttctatgacg gattttatcg cgtataaata cgatttgagt caaaatgtta gttcttggat
      661 gcaaggtgac ttttctatga tacttaaagg aaattatgag atattatata tcacaatacc
      721 ccttataata ttagcatata tttatgcaaa taaatttact gttgttggta tgggtatgga
      781 ttttgcgaca aacttaggtc ttagttacaa aagaattgtc aatataggtc tgataatagt
      841 agcacttgtt acagtatgtg tagtcgttac agctggtaat atccctttta taggtctaat
      901 agttcccaat atcgtatcct tgtatatggg tgataatata agagcaagca tatggtatac
      961 tggtttactt ggagcgatat ttgtacttat atgtgatatt tttggaagaa ttattattta
     1021 tccatatgaa atatctatag ggcttacagt tggagttata ggaagtatac tattccttta
     1081 tttaatatta aggaggaatg taaatgaagc ttagtctcaa tcaaaaaatt tactttgtaa
     1141 gtatattact attcatatca atagcacttt ttataattta tggaatagac atgaatcatt
     1201 tagaatatgc attatcacaa agaataccaa aaatatttgc tatggtatta ggtggaggat
     1261 gtatagcatt tactactgta gtgtttcaaa caattacaaa caatcaaata ttaacaccaa
     1321 gtgtactagg tttagattca ttgtatgtaa tgattcaaac tataatagta ttcatatttg
     1381 gttcaagcag tagtctgatt ataaatgaaa attataattt tattattaat gtagctatga
     1441 tgattggagc ttcattgtta ttatataaga cgttatttga aaaaaataaa aataatattt
     1501 tctttttaat cctagtagga atgatatttg gaaccttatt taaaagtgca acaacattta
     1561 ttcaggtaat gatagaccct aatgaatttt tagctttaca aactagtata atggcaagtt
     1621 taaacaatat aaacactaat gtattgttaa tagcatttat aataattatt gccataatac
     1681 cttttatata tgatgaaata aagtatttag atgtattgtc gttaggtaaa gaacaagcaa
     1741 ttaatttagg tgtagatttt gataaagcag ttaaaaaaat gataatatta atagcaattt
     1801 tagtctcaat atcaacagca cttattggac ctatgacatt tttaggactt ttacttgcta
     1861 atataacaag agaaattttt aaaacatata agcatacata cttgatttcg ggttctatgt
     1921 tgatagggat gattacattg ataataggtc aattttttat acaacatgta tttaagtttg
     1981 atacaacttt aagtgtagta ataaatttta taggaggaat ttactttatt caattactat
     2041 taaagggggc aaatagatga tagagataaa aaatatattt aaaagatata agaataaaaa
     2101 tgttgtagat gatgtctctt ttagtataga aaagggaaag ataacatctt ttataggacc
     2161 aaatggagct ggaaaaagta cagtactatc aatagttaca agacttatag gaggtgatgg
     2221 tggagaagta ataatagaag gaaaaagctt gactaattat agcaataaag aattagcaaa
     2281 aaaaatagct attcttaaac aatctaacaa tataacttta aagcttacaa taagagaatt
     2341 agttggattt ggaagatttc catatagtga aggtaatcta actaaagagg atgaaaatta
     2401 tattgatgaa gcaatagaat atatgaagtt gactgatata caacataagt atttagatga
     2461 attgagtgga ggtcaaagac aaagagctta tatagcaatg gtaatagctc aagatacaga
     2521 gtacatatta cttgatgaac cactgaacaa tttggatatg aaccattccg tacagatgat
     2581 gaaggtactt agaagtcttt gtgatgagtt agataagact atagtattag taatgcatga
     2641 tataaatttt gcatcatgtt attcagataa tatagttgca ttaaaaaatg gaaaagttaa
     2701 aaaagtaggt cgtacagatg agatagtaaa tgaaaaagtt ttagaagata tttatgagat
     2761 gaattttaac attaagaata taaatggaaa tcgaatctgt atttattttt aaaatattgt
     2821 atatgaaaat aaacttgcta tatgattttt tataaagttt ttaaattaaa ataaagatta
     2881 ataggagaga ggaaaatatt gatatgaata aaaaagcagc aatagtagca gcagtagcta
     2941 ttataggatt agtgacagtg tttgcattag gtggaagtaa aaagaatgaa tcaaaaacat
     3001 cagaaaattc aaataatact atgaagataa ctcataactt gggagaaaca gatgtaaaat
     3061 taaatcctaa aaaagtagtg gtatttgatt attcggcttt agatacaatg gatgcattag
     3121 gtgtagctga gaatttagta ggacttccaa aagcgagttt accagcatcc ttagaaaaat
     3181 ataaggatga caagtatgca gatttaggag gattaaaaga accagattta gagggtataa
     3241 agtcggcaaa tccagattta attataataa atggaagaca agaagatttt tatgagcaat
     3301 tgtcaaaaat agcaccaaca ataagtacaa gtaaagatga taagaagtac ttagaatctg
     3361 ttaaaaataa tatagacaaa atagcaaaaa tatttggagt agaagaaaaa gcaaatcaag
     3421 aatttagtaa aattgaaaag aaaatagaaa ctttgaataa aaaagtaaca gataaaaatt
     3481 taaatgcttt aactataatg gtaaatgaag gtaatttaag tgtatttggt gaagaatcaa
     3541 gatttagtat attatacaat agttttggat ttgaaaataa agataaaaat ataaaagaat
     3601 caagtcatgg acaaaatatt acttttgaat atatagctaa acaaaatcca gaagttatgt
     3661 tcgttataga tagaggaata gctactggca gtgatgtaaa agaaagctca actgcaaaat
     3721 ctgttttgaa caatgatata ataaaatcta tggatgcata taagaatgat aatataatat
     3781 atttagattc accaacatgg tatgtaaatg atggtggtct aacatcttta aataaaatga
     3841 tagatgatgc ttcaaaagcg gtaaattaat aatccgatat aatcaaaata tattttttat
     3901 aaaatgatta tcaaataaac taggagtttt taagaactcc tagtttattt tttgcataaa
     3961 aaagatatag tttaaacaat aatattatct ataataagat aaatctattc aaaatctaat
     4021 aaaatatgaa gttatactag acaaaaagaa taactcttaa atataattaa gttatataat
     4081 aatatataat tataatgata taattttaat aggtggtttg taattaaaaa gtttataaat
     4141 tatattaata gtaataatct ataagaactt atatattaga aaaaattt
//
LOCUS       DAEMGG010000207         4162 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEMGG010000207 DAEMGG010000000
VERSION     DAEMGG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.208"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            28..411
                     /locus_tag="KPK24_003649"
     CDS             28..411
                     /locus_tag="KPK24_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301279.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            919..1302
                     /locus_tag="KPK24_003650"
     CDS             919..1302
                     /locus_tag="KPK24_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2301280.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1304..1654
                     /locus_tag="KPK24_003651"
     CDS             1304..1654
                     /locus_tag="KPK24_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301281.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFVDKK"
     gene            complement(1755..2213)
                     /locus_tag="KPK24_003652"
     CDS             complement(1755..2213)
                     /locus_tag="KPK24_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301282.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2258..3046)
                     /locus_tag="KPK24_003653"
     CDS             complement(2258..3046)
                     /locus_tag="KPK24_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2301283.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3185..3622)
                     /locus_tag="KPK24_003654"
     CDS             complement(3185..3622)
                     /locus_tag="KPK24_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HBG2301284.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
BASE COUNT         1536 a          592 c          506 g         1528 t
ORIGIN      
        1 attatcttag aaaggaatgt gctttatatg tctacagaca aagttgttgg aatcatagat
       61 gaagaaacgg cagaacttgc aggaattgaa tatactggta aaatttatgc ttcttctggt
      121 gttataaaac atataaagaa aaaacataga tgtcaactga gtaaggatat ttttaatgat
      181 ataatagaca ctataaaaat ggtattaaag tcacctgaat atataggaag tcatcctaaa
      241 aaacctggaa aaagtgtgga gtttataaaa aaaattgatg attatatttt agtagctaca
      301 gaattagata caaaaaaagg atatttatat gtatcttcaa tgtatgctat aaaagaagca
      361 aaattagaaa gtagagtaaa cagtggaaga gtaaaacaat atgatggtta acataatatt
      421 attaattttc cctaaaatat tgaaaaatat tattatataa gttataatat taatatacac
      481 gtagatattt attagttaga tttgaggttg gaaaaggcac ccaatacgct actatagagt
      541 agttaataga gatgcaggat acgccgccct gccaaatcta attaaattaa aaagagagga
      601 ttaattcctc tcttattgtt tttttgagat actaatttaa tctataatag aaagattgaa
      661 ggtcgtttat acaaaaaaat tttcagttat atacattaaa ctaattaaat aagatattta
      721 taatctatat gtgatacata ttatatatta ataaaagaaa aatatataga aaatccttat
      781 aaaaatttaa attgtaatga ataggaagta attaaaaaga atttttattg atttttaaaa
      841 atttagttag aaactatatc attatggtaa aaaatgtatt ataatgttta gaaatattat
      901 aataaggagc gattaaacat gaatctatcg aagaaaatag aagataaaag aaaagctaaa
      961 cagagagctg aaaaagttaa gaaagctaaa attgctacag ctggtgtggt attaggagct
     1021 gttactggtg cagtgagtgg agtacttttg gctcctaaat ctggaaaaga aacaagagag
     1081 gatataaaag atgcttcaca acaaattgct gaaaagataa atatgaaaac tgtagatgta
     1141 aaaggcaaag tatctgagaa gctagaggat aaaaaaggaa actttataga atcaaagaaa
     1201 aaaataaaaa aatatataga taacaaaaag agtgttgaaa attctagtga ggaagataaa
     1261 ataatagata tagttgaacc agttagtgtt actgaggaat aatatggaca atacaataac
     1321 gattaacata agtataatat atgtattatt agctatactt gcttgtattg ctttagttta
     1381 tttaattatt gtacttgtta aactacataa gattttaaat aatgttggaa ctatgttaaa
     1441 tgaaaatact caaaatataa atacaacttt aaataatctt cctaatgttg tagaaacttt
     1501 tggagaagta ggagaaaata tgaaagatgt aacagaagta gttactgaag ttacagcaga
     1561 ttttcttgtt actaaagaca gtgtcaaatc aaatatggat gttatagttg atatattgac
     1621 tataattaaa aacatttttg tagataaaaa ataataagtt aatataaaat aagcctataa
     1681 gatataattt aaacgacctt aaaatagcat ttataaggtc gttttttatt agcttgattt
     1741 gacactatgt gtttttaata attggtaata ttcctcttta tcatttgaac atatatatct
     1801 atgcttgttg tttcaattac atccataaga acatctttac attcaagctc aaaatcttcc
     1861 attccttcta aatattcatc atcttcatca aaaaagatat aagaattata tagccattct
     1921 tcaaattttt cttcaagtat atcccaatac gaactccaaa ataatccaca acctgatata
     1981 taagatgctc tactttgatt tatataaact tcatttagaa gcttttgtaa tctactgcta
     2041 taaagaaaat ctgctcttcc actgcttgat attaattcat ttaaatcttc tattgacatt
     2101 aattcaactt taccactttt atcataagga gtataatttt catcaaccca tttatgtggt
     2161 ttgttttctc tgcatactgt tgctatcaac tctacaactt ttgttcttat cattttttta
     2221 tcctaactta tttatttttt ataaaataat catgatttta taatttatct atttcttgta
     2281 atgctaattt aacagtttca tatctatcag caccatttat tactacatca ccttttacaa
     2341 gtttattagc ttctccacct acaacataaa cctttttacc taaaccttgc ttataactag
     2401 acgcttcaat tattaaagaa tctttcaatt gccaatgtaa aatctgagca gccactttat
     2461 cagcatcacc tgtataaacg attacatttt catacacttt attttcccct cccccttgag
     2521 aatttgatat agatttgttt agtatacctt ctgctatcaa tttagcaact ttatctttat
     2581 tattaacata ataatctgta tctgctttac tatctacaaa acatacttct attaatattg
     2641 ctggtgcaat agtatgatta agccaataaa gacctctaac atcagacttt gcacccctat
     2701 ctttgaatac tgtagctaac ctagtattca ctctttctgc ataggttttg ccattattag
     2761 tcttatatat tgtttctgtc cctactggag ttgaagttgt agcatttgaa ttaaaatgaa
     2821 tctgaacagc caaatcagtt tcctgtttat ttgctatagc acattggtct gctaaatgat
     2881 tacttgattc atcaactctt cctgtataaa cagtagctcc acctatttta agccatttaa
     2941 caattaagtc attcagtatt cttgtttctt tgctttcgtt tatataccca acagctcctg
     3001 taccttttcc aattaaagtg tgccctggta ttattactac tttcaaacta acatctcctt
     3061 tacaattaaa tttaacttct atttaatata tgcataacct agaattttgt ttctttttat
     3121 atcttatact tttattataa cccattttgt aaaaatatag aatattaagt aaataatgtt
     3181 aaaattataa ttttgtatat aagacattac ttgattgcaa taattttata gcatattcat
     3241 ctattctata tccagttcta taatatatat gtttaattcc agcttgaatt atagccttag
     3301 tacaatttaa gcatggaaag tgagttacat aaatagagca atttttaact gaaatacctt
     3361 cttttgcaca ataataaagg gcattttgtt cagcgtgaat ggttctaata caatgtccat
     3421 ctctcatttc atgaccaact tcactacaat gcttatctcc agaaatacta ccattatatc
     3481 cagtagacac tattctgttt tctgaattga caattatagc tcctacataa gctctatcac
     3541 aagttcctct ttcagctact gtttcgcaca gtctcataaa atattcttgc catgagcatc
     3601 tattttcaag ttttttattc aaatcatata cctccaaaat ttatatctaa tatataagat
     3661 ttatacctta aaaataatat taaaaacaaa tttatatgat aaataaaatt tattaatact
     3721 gtttttagtc taaattattt cattttaaat taaagttaca aggaatctaa gattatatat
     3781 atctttcttc gtaatatgaa tgttacaata taaaaaactg tctcaataaa atagagacag
     3841 ttttacagtc aaagtttata atacaagttc atatttttct ttaaattctt ttacagcttc
     3901 gggccatgcc ttttgttctg taataccttt ttctttagct attttaattg caatctctct
     3961 tatcattgca gcgccttcta atattcccat ttttaatctc ccttcaaata tctttttgta
     4021 tagaatataa atagtgctaa gatttattac atgctgcata tacaatatct atacaacaca
     4081 taacttgtaa attcctttta ttttcatacg tctttaaccg acatataatt atattcaata
     4141 agttttaaaa ttatgaactc aa
//
LOCUS       DAEMGG010000208         4150 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEMGG010000208 DAEMGG010000000
VERSION     DAEMGG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.209"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            270..1673
                     /locus_tag="KPK24_003655"
     CDS             270..1673
                     /locus_tag="KPK24_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2301285.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1764..2004
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2047..2745
                     /locus_tag="KPK24_003656"
     CDS             2047..2745
                     /locus_tag="KPK24_003656"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG2301286.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2768..3769
                     /gene="trpS"
                     /locus_tag="KPK24_003657"
     CDS             2768..3769
                     /gene="trpS"
                     /locus_tag="KPK24_003657"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG2301287.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1569 a          458 c          737 g         1386 t
ORIGIN      
        1 atactaatga aaaataataa ataaaatttt ataataaaat aatattgaaa caattttaaa
       61 aatttagtat cattagaatt aaagtggtaa aacttttaaa tttatcattt ttaataagat
      121 ttgatttcaa agtatttaag gtttattatt aaaactgttt ttgaatatgc aaatattttg
      181 aaaaatatca gtttaataat aatgaatcta ttttattaat tttgaaatta aatttaaaat
      241 aattaataga gaggggaaat taaattagga tggaaaagaa aaaaataggc ctatggagtt
      301 tggtatttat gaatgtatca gctttatatg gtataagatg gatagctaaa tctacagcat
      361 caagctttgg tttaggatta ggtgcaatac ctatgtggtt attattctca tttatatact
      421 ttgtaccagg agctttaatt tgtgctgaat tagcttctac atatcaaaaa ggtgatggag
      481 gtctttacga ttgggttaag gaagcctttg gagaaaagta tggattttta gtttcttggt
      541 taaactggac agctaaaata ttttggtatt cttcattttt aactttctta gcagtaaatg
      601 tttcttatac aataggaaat ccagcccttg ctgataataa gatgtttgta cttatattat
      661 ctttaataat attctgggca ttatctctta tagcaactaa aggaataagt tttacaaaaa
      721 tatttactaa tacaggtgca ttaggttcaa caataccaag tgctatactt ataatatttt
      781 catttgtagc agtatttgtg ttgaaaaaac atgatatagc ttctacatac acaatagctt
      841 caatgactcc aaaacttaat atggatgcat ttgttgctat atcagctata atgtttggtt
      901 tagcaggagc agagactgct gctaacttca taacagaaat agataagcct gaaaagaact
      961 tccctaaggc aatattaata tcagctggaa tagtagcttc attatatgta ttaggttcaa
     1021 ttgcaataac aatgataata ccaccagacc aaattactgc ttctaaaggt gtattagatg
     1081 cactttctgc tgtttgtgcg tctttaggaa ttggttcttg gttggttcaa ttaatagctt
     1141 ttggtatagc tttttcagta ttaggagcaa tagttcttta catagcatca ccaataaaaa
     1201 tgttatttgg tagtgttaaa aaaggaatat ttccagatag tttagtagag gtaaatgaac
     1261 acaagatacc ttctaaagca gttatacttc aagcaataat agttactata atactattgg
     1321 tgactacatt aatgccttct gttgatgcta tatacaatgt tcttgtaact atgacagcat
     1381 taactgcatt attcccatat gtattgttat atgcttctta tattaaacta agaaaagaga
     1441 gaccagatga aattagacct tatactatgg caaaatcaac aagtacttgc ctaggtttag
     1501 caaaaatggt tttagttgta actgtagtgg gtatattatt atctgcagca ccagttatgc
     1561 ctacatttgc tgaaaatgtt atatatgaga tagagatgat tggtggaggt cttctagtta
     1621 tattatctgg tctatggtta tggaatagat atgaaaagaa aacagctaaa taaaatgtat
     1681 tttaaaaatt tcttttggat attataatat atagttgaca atttatagtg taaggaatat
     1741 tataaaataa agaaattaat aaattaaatc ctgagataag aaatagtaaa taaatatatt
     1801 tttgcacaga gaattaagga tgctgagaac ttaataaaaa gtatttattg aatgggtctt
     1861 tgaggagtaa aatgaaatgt aatttaatat tacagagtat ttgatatcgt gtatgcacgt
     1921 tatagcatat attaagatat atatattcaa ttttgagtgt atataaattt aggtggtacc
     1981 acggaaaata tatccgtcct attgatttag gacggatttt ttaatataga aaatttagga
     2041 ggaaaaatga acgtaatagg aattataggt gcaatggatg aagaagttag tattttagtt
     2101 gatttaatgg atattagaga gactataaaa aaagctagct tagaatttta taagggaatt
     2161 ttggaaggta agaatgtagt gttagtaaag tgtggcatag gtaaggttaa ctctgctctt
     2221 tgtgcccaga tactaataag cgaatttaag gttgatgcta ttgttaatac aggtgttgca
     2281 ggagctttaa acgaaaaact tgatgttaat gatatagtaa tatcaactga tgctatacag
     2341 tatgatgtag acactacagc atttggagac cctaagggag ttataccaag aatgaagact
     2401 tctgtattta aagcagatga aagacttata gatgctgctt acaaatcttc tgtggaagaa
     2461 gtaaagactc ataaagtatt aaaaggaaga gtagttacag gagataaatt tataaattct
     2521 aaagaattaa aagaagaatt agtaaatgat tttggtggat attgtggaga aatggaaggt
     2581 ggagctatag ctcatgtatg ctatttaaat aatactccat ttgtaataat aagagcaatg
     2641 tctgataagg cagatggaag tgctgatgtt acttatgatg tatttgtaca tgatgctgcc
     2701 aataactcta aagacatagt tttaaatatg ttaaaatcaa tataaatata taaggaggta
     2761 caaaaaaatg agtgaaaaaa aagttatatt tagtggtgca cagccatcag gcaaaatgac
     2821 tttaggaaat tatttaggag ctataaaaaa ctggactgaa cttcaagaca attatgattg
     2881 ttattatagt atagttgatt tgcatgcaat aacagtacct caagacccta aagttttaag
     2941 agccaatact atagaattac ttgctcaata tatagcttgt ggattagacc cagaaaaaaa
     3001 tactatattt atacaatcac atgtcaaaga gcatgttgaa cttatgtggg tattgaatac
     3061 tatgacttat atgggtgaat taagtagaat gactcaattc aaagataaat cacaaaagag
     3121 tgaagcaaat ctaaatgcag gtttattcac atatccagtt cttatggctg ctgacatatt
     3181 gttgtatcaa actgacctag ttccagttgg agatgaccaa aaacaacatt tagaattagc
     3241 tcgtgattta gcaaatagat ttaataatag attttcacct acttttgtag ttccagaagg
     3301 atactaccca aaaggtggag caagagtgat gagtttgcaa gagccaacta aaaaaatgag
     3361 taaatccgat tcaaatgaaa atgcatttat actattagca gatgattcag attctataaa
     3421 gagaaagata aaaagaagtg ttactgattc tttaggtgtt gtaaaataca atgatgaaca
     3481 accaggtatt aaaaatctaa ttgatatata ttctaattta agtaaaaaga gtgtagaaga
     3541 gattgtaaac atgtatgaag gaaagggtta tggcatattt aaagaagatg tggctgaagt
     3601 agtatcagaa gctttaagac ctataagaga aaaatatgtt gaccttctta ataataaaga
     3661 ttatttagaa aaaatatata gtatgggagc agaaaaagcc gaaaaacaag ctagaaaaac
     3721 tcttagaaaa gtttataaaa aagttggatt tatagagaga agatactaat tattagctat
     3781 ctttacttga taaataaatt ttaaagttat taaaaaggta tatgatatga aagagagagt
     3841 ctcaaagtga acttttgttc atgaggcact cttttttgta tgaaataaaa tatattttaa
     3901 tcatttcaac aatgaaaaag aggattatct ctattttgag acagcatctt ttgataataa
     3961 aaaaagatga gcttttgctc atctcaaagg ggttagatta ttcttacttt ggggaaagta
     4021 gaataaaggt aaaagttaac tgatgtcaac tttgtctttt ttgcatatca ctactgagca
     4081 taaaaaaatc agaaaaccat agccttctga aacaacttct aacatacatt gtattttata
     4141 cccaatatgt
//
LOCUS       DAEMGG010000209         3967 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEMGG010000209 DAEMGG010000000
VERSION     DAEMGG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.211"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            6..404
                     /locus_tag="KPK24_003658"
     CDS             6..404
                     /locus_tag="KPK24_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2301288.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNIK
                     INLNIKGYKFLPYVKNLQASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            392..1330
                     /locus_tag="KPK24_003659"
     CDS             392..1330
                     /locus_tag="KPK24_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG2301289.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFRLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1714..1938)
                     /gene="secG"
                     /locus_tag="KPK24_003660"
     CDS             complement(1714..1938)
                     /gene="secG"
                     /locus_tag="KPK24_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG2301290.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2531..3823)
                     /gene="eno"
                     /locus_tag="KPK24_003661"
     CDS             complement(2531..3823)
                     /gene="eno"
                     /locus_tag="KPK24_003661"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG2301291.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1356 a          642 c          451 g         1518 t
ORIGIN      
        1 ttgccatgat aaaaaaaata gaacatgtgg tcattactaa taataatata gaaaattata
       61 tcaattttta taaaaaattt ggtttttcat ctaaagaaat gagcaaaaat tataaactta
      121 atgctagaaa tattaagata aatcttaaca taaaaggtta taaattttta ccttatgtta
      181 aaaatttaca agcaagtaga gataacttat gttttaaaat aaacacaaat attgatgagc
      241 caaaaaaata cttagaacaa aatgagattc aaattaagcg aggaattgta gaatgtacag
      301 gtgtctttgg taatatgaaa tctatttatt tgaaaaaaac taatggaaat ttaattgaat
      361 tatcaaatca tagaatcacg gagagtaaac cttggaaaat ttaatcctat caataaatgt
      421 tgttcttcct ttgtttctca caatgtcact tggatatgtt ctaaaaaaat taggtctatt
      481 tgatactcat actttaaata aaatgaataa tgtctgcttc aagtctttct tgccattatt
      541 attatttttt aatatataca atacagattt agatggttct cttaatttta ggcttatggc
      601 tgtagctgtt ataattattt tttcaatctt cttaatctta gtctttatta ttcctaaaat
      661 tgaaaaagat aataagacaa ggggtgcatt aatacaaggt gtatttcgta gcaatttcgt
      721 tatatttggt attcctgtga caatttcact atttggcgat aagagtattg gagtcacatc
      781 tttattaatt gcagttattg tacctatgtt taatgtatta tctgttgtaa ttcttgaaat
      841 ttttagaggt ggcaaagtaa atataaaaca tatgattaaa agcgtcttaa caaatccact
      901 cataatcgcc tcttttatag gattgacaat gctaatttta aatattaata tacctacatt
      961 tttacacaaa actattggtg atatttctaa aatagctact cctttatcac ttataattct
     1021 aggtggctct tttagttttt ctgctataaa ggggcatata aaccccatta tattgggcgt
     1081 atgtggaaaa cttatacttg tacctgctat atttttacca attagcattt ttcttggatt
     1141 tagaaatgta gaattagctt cacttatgat aatgttatct gctccaactg cagtttcttc
     1201 atttacaatg gcacaacaga tggattctga cagtgaatta gctggacaaa ttgtagtatt
     1261 tacttctgga ttttctataa ttacagtatt tttaataata tttattttaa agcagaataa
     1321 tcttatttaa tgatatttga taacacaaat tggtattatg agaaacatat ataaaatcga
     1381 tttaattgcg taaaaacata ttattatact atttttacta tataatataa ctttgtgtat
     1441 attatctcat taaacatggt gaaagtagtg ttgctatata ttttttagtt tgaaaaaaat
     1501 atataaatac aaacaatata taaatacaaa taataaacgc caacaaatag tcaatctata
     1561 aaataaaaac tggactaatc atataaaatc attagtccag cttttatttt acgtactttt
     1621 tatgttgatg tacttttatt aaaaactaaa tttaattttt aaatgtctag caaatgcact
     1681 aattaaataa tggccagtta ttcaacaata tcactacttc ttaactataa gtaatgccaa
     1741 tgcatttata aaaaataata ctgctgttat ctttgtaact ctagcaagaa atgcttgttt
     1801 tccttttggt ttaaagtaag cttggcttcc ttctccacca aattctgaag gtaaagcatt
     1861 cttagaatct tgaggcatta tgctaattac tagtaaaatc cctagtacta cttgcacacc
     1921 cattaaaatg ttgctcattc ttgcccctcc tatctgttta ttacttaaat ttcattactc
     1981 tttagtgtat cacaataata tagccatttc aagactatta tgctataaat aacatacaaa
     2041 aaaatatatt gttactataa tatattacta tattctcaat ataaaaatga ataaaactta
     2101 gaagagaaaa ctatttaaat taatacataa attaacactt aaaataatct actctcattg
     2161 catatattat aaatagccac caaaataaaa caatcatttt aaaatattga tataactttt
     2221 aatttcaaca taatttaaaa taaataatcc acatttagca ataatacttt aatgacaatt
     2281 atgattaaat caaaatatgt cttcactttt attttttaat aaaaacctcc tatgcaatta
     2341 gataaaaatg agatagaaac atttattcat attttttctg aatctatgaa taaaattaag
     2401 taataaaata taaaaaagga taagtgaatt gaaaaatcaa ttaacactta tctcttttat
     2461 atcttataag atgagcttaa atatcttata ttctctcttc attaatcttt tttagtaacc
     2521 attttaataa ctattttttt aagttataga aagattttaa tccacaatat ctagcttgct
     2581 ctccaaccat ttcttctatt cttaataatt ggttgtactt agcaactcta tcagttcttg
     2641 atggagcacc agtttttatt tgaccagcat ttactgcaac agctaaatct gctatagtag
     2701 aatcttcagt ttctcctgat ctgtgagata taacagcagt atatcctgct ctcttagcca
     2761 tttctatagc atctaaagtt tcagttattg tacctatttg attaacttta actaatatag
     2821 agttagctac tccattttca attccttttt ctaatctctc tgtgttagta acaaataagt
     2881 cgtcaccaac taattgtaat ttatttccta atttttcagt taataatttc catccatccc
     2941 aatcttcttc gtctaaacca tcttctatag ttattattgg gaagttgttt gaccaatctt
     3001 catataaagc aaccatttca gcagcagtta actcttttcc ttctcctgct aaaacgtatt
     3061 ttttagtttc tttgttgtac atttctgtag cagcaacgtc aagacctaac ataacgtctt
     3121 cacctggctt atatccagct tttgttatag cttcaactat taattctaaa gcttctctgt
     3181 ttgaacctaa gtttggagca aatccacctt cgtcacctac accacaagct aatccttttt
     3241 ctcctaaaac tttctttaat gaatggaata cttctgctcc cattcttaaa ccttctttga
     3301 aacaacaagc tcctactggt aatatcataa attcttgaac atcaacgtta ttatcagcat
     3361 gctctccacc atttaatatg ttcatcattg gaacaggtaa ttgtttagca tttactccac
     3421 ctaagtattg gaataatggt aaacctattt catcagcagc tgctctagct actgccattg
     3481 aaacacctaa tatagcatta gctcctaatt tacctttgtt tggagttcca tctaattcta
     3541 tcatgattgc atctatagct ggttggtcaa atgcatccat tccttctatt tcaggtgcta
     3601 ttatttcatt tacgttagca acagctgttt ctacaccttt tcctaagtat cttcctttat
     3661 ctccatctct taattcaaca gcttcaaaag ctcctgtaga tgctcctgat ggaactattg
     3721 ctcttcccat tgctccatct tctaatacta cttcaacttc tacagttgga tttcctcttg
     3781 agtctaatac ttctctagca tatactaatt caataactga catttgtata tctcccttcg
     3841 attttctatt agttaaattt tcatttactg taaacatccg tctactattt taatctactt
     3901 tctatattat agtacataaa gttgcttttg aaaattaggt acaaatttat ttgatgggta
     3961 aatttgt
//
LOCUS       DAEMGG010000210         3948 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEMGG010000210 DAEMGG010000000
VERSION     DAEMGG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.212"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            189..1694
                     /locus_tag="KPK24_003662"
     CDS             189..1694
                     /locus_tag="KPK24_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2301292.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTIPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTVKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1808..2506
                     /locus_tag="KPK24_003663"
     CDS             1808..2506
                     /locus_tag="KPK24_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG2301293.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2791..3879
                     /locus_tag="KPK24_003664"
     CDS             2791..3879
                     /locus_tag="KPK24_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301294.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         1557 a          388 c          638 g         1365 t
ORIGIN      
        1 aaaaaaatag ttaatataaa agtgatacaa taataataga agtaataaaa aaaaataatt
       61 aatataaaaa tgtgtacaaa ctaatgaata ttgatatagt aagctatcat atgaaagagt
      121 atgtacacat tttttttact aatttttcac aatatgaaca taaataattt acaggaaagg
      181 aatcttttat gaaaattcaa aatactgagt ggggatatat tgaatggaaa catacttatg
      241 atgaaaataa tccaaaacaa gctatgaata tatacattgc agtaactatt cctggtaaga
      301 aacattttaa tcatgttcat tatggtcaag agcagatgat atatatttta gagggagagg
      361 gtttgtatat tataaatgga gtttggaagc cattttatca aggtatgatt ttttatatag
      421 aatctggtag cacacatgaa actataaata caggtgatag agaaataaaa gaacttattg
      481 tttcaaataa tgtagatgat gtaggagaga gtgaagttat agatattaat cctaataatt
      541 atttaaagaa gaccttgata aattatagtg aaagtacatt aaatttgtat gcagcagtag
      601 aatcaattcg tggtcaattt atagacccgt ttaaaattcc attaattata tatgatgatt
      661 catggaatat agttttaaaa aatccttatt ttccactttt ttgttttgaa aaatgtaatc
      721 ctatgaaatt tcctcaaaat tgtgattgta tgaatcaaaa aagtagcaat caatttgtat
      781 gtgaatatgg gataactata tataatatac caatactgta caaaagtaat agcataggtg
      841 ttataagagg agggtatgta ctattatctg atttgaattt agatacagag cataataatt
      901 tatatgatat tccagaggga gctgctagaa gtataaaacg cctcttgaaa caaatatcta
      961 aaaatataat aaatttttgc tcttttaatg atataagaaa ggatttacaa gaaaaagaga
     1021 agactatagc aagaacatat cattatggag agcaacttga aatgaattta aaagtagctc
     1081 aagatatggt tactaattta agaataaatc atcacttttt atttaataca ttaaattcaa
     1141 tggcaagcat agcgctagat gatggtagtt atgatttgta tagtgcaata atagatttat
     1201 caagaatgtt tagatataca atgaggtctg atttaagatt tgtagaatta gaatctgaaa
     1261 tattatacat taaaaattat ttgaatttac agaaattacg ttatggagat gcacttaaag
     1321 taaaatatct tattccagag aagttatata acctttctgt gccttttaac tttattcaac
     1381 ctattgtaga gaatgcattt acacatggat ttagagatat tgacacagta aagcgaattg
     1441 aaataattgc aagattagat tctcagtatg ctattataga gattcataat aatggaacta
     1501 tcttagatgg aaataatata gacaaaataa aggcaggtat tcgtagtaat aatggtcatg
     1561 gtttgtcttt aatatataca aaattcacat ctgcttatgg aaataatttt gatatggata
     1621 taaagtcgag tgataatgaa ggaacttaca taatgattaa aattcctatt gaaaattata
     1681 aggagtatgt atagaacttg tgaaaaatta ataagtttta taaatgttaa gaaatatatt
     1741 agatgttttt atgttgtttt atatatctta cttttttata ttattaagct aaaagggagg
     1801 tattctcatg agaagagcac taataataga tgatgaagaa gctgttctaa aaataataac
     1861 acattttata gaaatgaaag atatgccaat aaaaattgtt ggtaaagcaa catctggtga
     1921 tgaagcagta gataaaatca ttggtttaga gccagacata gtttttatag atattcaaat
     1981 gccaatatac aacgggttag aagtaataga aaaaacatct catttatcta aaaaaataaa
     2041 ctttgtagta ataactgcat ttaactattt tgaatatgct caaaaagctt tgagattaaa
     2101 tgtaaaagat atactcctta aaccattgga tatgaaagaa tttactaagt ctgtagaaaa
     2161 aattattgga tatcaatata ccaataatga tttattaaat gagatactgg aatatataaa
     2221 tctaaactat agcaatgact taaagttaaa tgattgtgct agattatttt taaccaatcc
     2281 atctaatata tctaggattt ttaaaagtaa tttaaataca acttttatat catatctaaa
     2341 tcatataaga atagaaaaat ctattgagtt acttgaaaat actacaaaaa gtataaatga
     2401 gattgcagaa atagttggat ataatagcct aaataacttt tataaaaact ttaagataga
     2461 aaaaggaatg actccaaaag tttataaact gaacactaaa aattgaaatt gataaataaa
     2521 aagtcaatat agataattgt ttgagtgtgt ctgaataagt atactttaat tatgataaaa
     2581 attaaagcgc tttaaacata tttaataata ttaaacctta taaaagctgt acctatttat
     2641 ataaaaaaat atatataaat aaaaagaaaa agatatacaa aataaaaaaa gtgaattaaa
     2701 tactgtttta aaaatacaga attttcattt aattcactga gagtttatat ttcttaaagg
     2761 aaggtaactt tataatggag gtaatttaat atgtcagaca aagtgaatat gaaaaatatt
     2821 atctgtattg caggcgctta tctagcaaca gcaattggtt ctgggtttgc tactggtcaa
     2881 gaaattctac aattttttgc ctcacaaggg tatatgggtt taattggagc attaatatca
     2941 atggtattgt tttcatttct tggtgctgaa gttataacta gaggtagaga attaaaactt
     3001 aaagaaccaa caagaattta cactttttat tgtgggaaga ttttaggaaa attttacgaa
     3061 tggtttagtc caatattttt atttgggatt cttatagtaa tgatttcagg tgcaggctca
     3121 actttaactg aatactatgg tataaatcct tacttaggaa gagtattgat ggctgttgct
     3181 actttaatta cagtgtctat gggtttagaa aaattagtaa gcatacttgg acgtattgga
     3241 ccagtgatag cagtatttac aattgctgtt ggattaataa gcttgtttaa gcattttgat
     3301 ggtttatcta tggttggaga agctatgaaa aatattgaag ttacaaaagc atcagaaagt
     3361 ccaatcgttt ctggtgtaat atataataca ttaaatatta ttgtaatgat tgttttttta
     3421 acaggaatgg gagcttctat tgaaagtaaa aaggatgctt tttggggagg cttattaggt
     3481 ggaatagtgt ttatgactgc tggtatagtt atgtatttgg ctatgatttc agatatagga
     3541 aatttgtata tgaaagaaat accatcactt tatttagcag ataatatatc tcctattgta
     3601 ggtgtatgtt tttcagttgc attaatatta ggtatctata caacagctgt tccactttta
     3661 tggtctgtaa ccaatagaat tgttgaagat gaacatccta aatttaaatt ggtaacaaca
     3721 gtaatagcta ttttggcttg tataggaggt tttttaccat ttggtaaatt agtaaacatt
     3781 ttatatccat atacaggtta tatggggctt ttaattttag tctgtatggt gtataaagca
     3841 attacatgta gaaaaaaatc aacacaaagt caggaataga gccacttatt aatagttttt
     3901 atttaaagaa attttttata taatccgaat ttaaaaagtc aaaatagt
//
LOCUS       DAEMGG010000211         3943 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEMGG010000211 DAEMGG010000000
VERSION     DAEMGG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.214"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1220
                     /locus_tag="KPK24_003665"
     CDS             <1..1220
                     /locus_tag="KPK24_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2301295.1"
                     /translation="LKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKV
                     NMLLISNLIYKLKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYF
                     SEHNNEKEYEELQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKIS
                     SKLEKYEGIIIPSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVI
                     SDDLYNEVKGEFSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMY
                     LVELQQSKILQFMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMN
                     KIVSIEIGTLFFIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYL
                     LKKNYTRKVWFFD"
     gene            1339..2730
                     /locus_tag="KPK24_003666"
     CDS             1339..2730
                     /locus_tag="KPK24_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2301296.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            2837..3877
                     /locus_tag="KPK24_003667"
     CDS             2837..3877
                     /locus_tag="KPK24_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG2301297.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
BASE COUNT         1588 a          401 c          649 g         1305 t
ORIGIN      
        1 agttaaaaat aaatggaggt atgtatacaa cagcactatt aattactttg gggatatttt
       61 tatttttttc tcaatttact attttgatat taaagtattt aaaaaataaa gaaaaatttt
      121 ataagaacaa agtaaatatg ttacttataa gtaatttgat atataaatta aaggataata
      181 cacgattgtt atttttaata acaatacttt tatcggctac tttagtagca tttacaacaa
      241 cttcaacact tgtaagttct caaggagaag cttctaaaaa taatttccca atggtttact
      301 catatttttc agagcataat aatgaaaaag aatatgaaga actacaacaa attagagaag
      361 caatcaaagg atatgattat aaggagttaa gttttcctgt acttaaatat aatagggaag
      421 tcttactaag tgtaaagaat tataacaaat tatcaaagga attgggatta gataaaatta
      481 gttcaaagtt agaaaaatat gaagggatta taattcctag tagcaattcg caagaacata
      541 taaattcttt aaaacaatta aaaaattata gtataaaaaa tttaaatata gaagtaaaaa
      601 aatcaaaaaa taaaataatt tttcctacag gactgtttag taaagttgtg gttataagtg
      661 atgacttata caatgaagta aaaggtgaat tttcaaaaat taattttcat ggatttgatt
      721 ataaaaactg gcaaagcagt gcaaagataa ctgaaaattt aaaagaaaaa cattttaatt
      781 tggatagaga atataacagg tcatattttt taacattacc agatatgtat ttagtagagt
      841 tacaacaaag taaaattctt caatttatgg gtgtttttat aggagtgata ttatttatag
      901 cagtttttag ttttttatac ttcagattat atacagacga gatagaagat aatgtaaagt
      961 acaaaaaact atcaaaaata ggtctatctt ttaaaaatat gaataagatt gttagtatag
     1021 agataggtac attgtttttt ataccgtatt ttatagcaat tataaatagc ataatttctt
     1081 tactgttttt aaatcaagta ttaaataata gttttaagtt agaaaatttg ggtatattac
     1141 tactgatatc atctatatat tcactttatt tttacttatt aaaaaaaaat tatactagaa
     1201 aagtttggtt ttttgattaa agtggtatat aatagatata taaattaata tttaaaaagt
     1261 agttaatatt aggggagggc atttgcacct cccttgtaat atgtggaaaa ttgaatatta
     1321 gaagaaggag gagacttgat ggaaaatcaa caattattag gaacagaacg aattagtaaa
     1381 ttactactaa aatattctat accagctata ataggtatgt tggtaaatag tttatataat
     1441 gtagtagata gaatttttat tggaaatata ccaggggttg gaccacttgc aataacaggt
     1501 cttggtgtaa caatgccaat aatgacaata atacttgcat ttgggatgtt gataggtata
     1561 ggaactacta ctaccatatc aataaaactt gggcaaggta aagtagaaga ggcaagaaaa
     1621 cttataggaa atgcaatgac gttatctgta ataacaggaa ttatcataat gatacttgga
     1681 atattatttg caaataaaat acttacattg tttggagcaa gtgaaaatac tttaatatat
     1741 gcaaaatcat atatcaatat tatattatta ggtacagtag taaatcttct ttccttttct
     1801 ttaaaccact caataagagc tgatggtagt cctaaaattt cagcaggaat aatgatagta
     1861 ggatgtttaa ctaatatagt tttagactgg atattaatat ttggattcaa tttgggaata
     1921 caaggagctg caattgctac agtaacttct caggctctta cagctatttt aactataggg
     1981 tattatataa gtggaaaatc aaacttacga ttcagtaaat ctaatctaaa attagataaa
     2041 aagctaataa aagcagtgtt tgcaataggt atgtcgccat ttgctatgca acttgcagca
     2101 agtttagttc aagtaatatc aaatattgca cttaaaactc atggtggaga tttagctata
     2161 ggggctatgg ctactatttc ttctattgca atggtatttt tgatgcctat ctttggaata
     2221 aatcaaggag cgcaacctat tataggtttc aattatggag ctgaaaaata tgatagggtt
     2281 aaaaaagcat atttaggttc attagtagta gctactataa ttttatgtat gggtatggta
     2341 gtagttatgc ttttcccaga agctattata ggtatattta ataaagaccc tgaacttatg
     2401 aatatatctg ttaatggatt aagaatatac ttacttatgt tgccaattgt aggcttatca
     2461 gttacaggaa caaactttat acaatctata ggaaaagcaa aaatggctat gttattgagc
     2521 cttttaagac aagtaatttt attgatacca gcagtattaa ttcttcctac attcttaggt
     2581 ttacagggtg tatggacagc tcaaccagtg tctgatttta tagctacagt aattacaggt
     2641 atagttgtat ttagagaatt aaaaagatat actcctaaaa ctgaaaaatt aaatgaaaat
     2701 gaaagactaa acgagataac tactgagtaa aaatataaat tcagataatt gagcaagaaa
     2761 ttgcaaaaaa tacgattata tgataaaata aaaccgctgt caaggataag agaaattcta
     2821 attatggagt gattttatgg aatttacaaa gaaaagcaaa ttaaaaaata tgtatgtaaa
     2881 catgaatgta gctagaatgt tgtctaagat aaatgagtat aagggtagac aactgcttta
     2941 caaaaaacaa ccaaaagaaa ttttagaaaa cttagaaaaa aaatctttag tagattgttc
     3001 tgaatctaca tacattggaa atcaaagaga tagtagtaat tttaatctag aaaagttgat
     3061 aagtaatgaa gttacaccta gaagtagaga ggaattgagt attgttgaat acagagatgt
     3121 ggtaaaaact attaacagtg cttatgagag tattcccatc agttcacaaa caattcttga
     3181 gcttcatgga tacttatata aattttcatc tactagagga ggaagttata agtcagataa
     3241 tgattttatt gaacacaatt taaaacctag cgaatttata agtactgatt caagtgtcaa
     3301 taaagttgaa aaagctgtag aagaaatatg tgaagcttat aatacattga tagaggaaga
     3361 tgaaattgat atattaatat taatttctgc atttgtgtta gatttcatat tgatacatcc
     3421 attcaaagaa ggtaatataa aaatggcgag agtacttatc ctattgttac taaataaaaa
     3481 tgggtatgaa gttggaagat atataagttt aggaaagata tttgatgata gttcatatga
     3541 atattatagt aatttaaatt atttgaaagc ctcaataggt agtgaaaaag cagatatgaa
     3601 tgcatggatt gagtattttt tagagacgat attaactgca tatgagaagc tggatgatag
     3661 tttaaatata tctgataaga aaagacaaac aaaaactagt agaatagaga aaataataaa
     3721 ttctaccctt ggatatttta caaaagaaga tataagagat ttatgtcctg acattccaga
     3781 acctactata aatagagttt ttaataatct aagaaaacaa gataagatag aagttgttgc
     3841 aagaggaaga agtgcaaaat ggaagaaaaa atattaaaaa atgtttagat aactaaaatg
     3901 gtatagtgtt ttacactata ccatttttaa tgtcattagc atg
//
LOCUS       DAEMGG010000212         3774 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEMGG010000212 DAEMGG010000000
VERSION     DAEMGG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.215"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(74..469)
                     /locus_tag="KPK24_003668"
     CDS             complement(74..469)
                     /locus_tag="KPK24_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG2301298.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            610..1002
                     /locus_tag="KPK24_003669"
     CDS             610..1002
                     /locus_tag="KPK24_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301299.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1102..1518)
                     /locus_tag="KPK24_003670"
     CDS             complement(1102..1518)
                     /locus_tag="KPK24_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG2301300.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1533..2444)
                     /locus_tag="KPK24_003671"
     CDS             complement(1533..2444)
                     /locus_tag="KPK24_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2301301.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(2626..3648)
                     /gene="splB"
                     /locus_tag="KPK24_003672"
     CDS             complement(2626..3648)
                     /gene="splB"
                     /locus_tag="KPK24_003672"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG2301302.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
BASE COUNT         1255 a          650 c          432 g         1437 t
ORIGIN      
        1 ttaaattaaa tctatcaaca tttcagtaat aacgttttct taattttcac tatggatgtc
       61 gattcaatta taattaaagc tctacatcaa tctttttacc actcattgaa caacaactaa
      121 cttttgcctt atcaatagag aatatttcaa acttaccatc tcctacagag tacatctgac
      181 ctaaacttgg cattatttca agtgccttag cttgtgtttc ttttgatgta acaaatactg
      241 cttcacctaa aatacgtaga gtattaaggt tttggtctat acaacaaagt tcaacctttg
      301 gattaaaaat aagttgctta aacatatcct tttgattaga tgtgcaaaat gtaagtttac
      361 cattgtattc cataacaaat cctaaaggac gcacatgtgc ttgattgttc tcatcaactg
      421 ttgctaaata ataaactcgt gccttgttca aaaattctaa tgctttcata attcttacac
      481 cccttttaaa tacttgtatt aaggattatc ctttgatata attttaatat aaaatacaga
      541 aaaaacaagt atgcagtttt ttctgataaa gtataaaaaa gatactacta taactaagga
      601 gtaattaaaa tgagtataaa tactgaaaaa aatataacta aaaacgttga agaattaacc
      661 tgtccaatcc gttatgcatt agacattgtc ggtggtaagt ggaaattacc tattatatgt
      721 atgttagcag ttgaaaatcc aattagatat agtagcataa aaagaaaatt ggatggcatt
      781 acaaatacta tgctagctca atcattaaaa gatttagagt ctactggtat tgttcatcgt
      841 aaacaatata atgaaattcc tccaaaagtt gaatatacat taacaagtaa aggtaaaagt
      901 attgtgccta ttttacaaca atttgctaat tggggtgcta ctaatatgca agaaaaaaac
      961 acatgtggac ttagttgcaa agaatgtaga aagataaagt aaatttaaat ttactaaatt
     1021 attcatatta tagactatat attaatttat ctgattgaat ataaaaacag gatgacttat
     1081 atattcatcc tgttctatat gttaagctct aaatttaatt agttcaagca tatcgtttaa
     1141 taccaataaa ctatctactt gaatcattag ccatttactt ccattaaaaa agtttacctc
     1201 attatatatt tttttgatat aatcacaaca agtattaata aataaatcaa cctctactct
     1261 attgctttca cttacaataa ccaaagcttt aaaataacct tcttgtggat ataaagtaca
     1321 tagtgattta cctctttttt tatattttac attccaacct ctttgcattg aacatttact
     1381 gtattcaagc ttaggttcta cattataagt agaaaccaaa gttttattta acttgtccca
     1441 taatggatta ttaataaatt cattaatttg ctcaaatgta ggtttagtat cttgaaaaaa
     1501 cgcatcttgc caattcatat tcatcatcct ttctattgtt caataggaac cggaacaatc
     1561 caatgttcca gatatgctgc ttcaggtgta gtggggtaat acatctcaat tgcaaaatca
     1621 gtagttgtta aattatgttt cttcatccat ctttccataa atttatctgc tttaaaaaca
     1681 gctgaattaa ctaactcatt aaagttttct gcctcaaatc cacatactaa atattcttta
     1741 gctggcaact caaaagtact atatccttct actgatgtac ctgactctac ctctacacct
     1801 gccatataca tacaatttcc ttcttttgca tttcccatat aaagagcagc aaattcattt
     1861 ccacctggaa tttgatttgg tatattatgt ctttgacttc caagtttcat ccataattcc
     1921 tctactattg caattccagt tgtttcaccg cccaccaact ggcaaatagg tacttctatt
     1981 tctattccaa caaatgtcct tggattactc aatgtcttac gattgacttc aatcactata
     2041 tcatctgcaa tcagtggaac accttcatca accatagcat aatttaaaat taattctggc
     2101 ttaataaagt gattgagttt tactggattt gctctatatt tttctggagt taatccatat
     2161 gcttttttaa aggaacgtgt aaatgtctca tgactagcaa atccaaaatt taaagctaca
     2221 tcaagtatac gattcttatg ttctgctaag tattcagaag ctcttgcaag acgacgaagc
     2281 tttatatact ccattacagt cttatttacc aatcttttaa atagtctttg atagtaaaat
     2341 ggtgataaat ttgctttatt tgctaattca ttaattgata tttcttctga taaatttgct
     2401 tctattaaat ccaatgtaat ttgaatagac tcccatgcat gcatactata actcctcctg
     2461 atatttttgt tactcttatc ctttatagta tcaaaactag aaaaaatgtt cttgatagat
     2521 agtgcccttt ttaattttct tatatgatag tgctaaatct cattgcttta ttataaacat
     2581 aaaatgtata aaaataaaat atagcaaatc tttaggtaag atttgctata ttatatattt
     2641 tattaaacta tttttaaata ctccatttat ttccttaaga aagaactctt tcatatcatc
     2701 taattgttct ttatcataca cgtacttacc atatccaaat tgaccgtatt taaactttct
     2761 atcctcattt tccattggca atagtgtatc tggataaatc tctagtattc tattttttgc
     2821 tttagttgta tatctatgag atataacttc aaatataatt tttttatcaa aatcctttgg
     2881 taaagcttct tttatactta taatcagatt tttatactct tctttccaac cttcatataa
     2941 aaacacaggt gctattatga aaccaatctg ataaccagcc ctagcaagtt ttacagctga
     3001 ctctattctt ttatttgcag aggctgtata atgctcatgt tcatttataa ttctaggagt
     3061 attgatacta aatcgtattt ctgtatgatt attatgttca gcatccaata agctatccac
     3121 atcactgtat ttagtaacaa atctaaatct tcctttttcc tctcttccaa aaaactctat
     3181 tgttttcttt aaagaattag tatatggctc tactggaact gggtctgatg tagctgcccc
     3241 ttcaaatatg gttacctgtg gctttctatc ttcaatatat ttttttgtct gttcaagtat
     3301 ttcatctaca tttacaaaca cccttataaa tggtttatca cctaattgtg tgttcaaata
     3361 acaatactca catcttccca tacatccact gacaagtggt agttgataat tagcagaagg
     3421 tttacatgtt tgaaacttca gactttttct aatcccaact accatagtct gcttcccact
     3481 tctatattgt tcatatagat tttcacctgg aatatgttct ttaatcctat ttgatgatat
     3541 attaattatc tctacatctt tattatctct aaatttattg tatatttttt tacccacatc
     3601 agtgtctaaa gttccttttt cgaatattat tcttttggga ttaaacattt atttcacctc
     3661 tacttaaagt ttttaaatcg aagaatttta taaaatatta cttaattaat cttccctttt
     3721 ttaaactcct ttatttatta gaaagtaaaa taaaagctta agacaaattt atta
//
LOCUS       DAEMGG010000213         3686 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEMGG010000213 DAEMGG010000000
VERSION     DAEMGG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.216"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            261..620
                     /locus_tag="KPK24_003673"
     CDS             261..620
                     /locus_tag="KPK24_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301303.1"
                     /translation="MKLRESYTCPLELVHDIIKGKWKTIIIFQLRNGKCSFSELHHSI
                     AGITQKMLLEQLRELKHFGLIDKKTYDGYPLKVEYFLTGQGEKMLSAIKIMQEIGIDY
                     MVEHGMTEILDEKGICY"
     gene            710..1261
                     /locus_tag="KPK24_003674"
     CDS             710..1261
                     /locus_tag="KPK24_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2301304.1"
                     /translation="MKKALLIIDVQNDYFPNGKCQLYKPEIALNTIKDLLKIFRKQNL
                     PIIYIRHESIHGTFFNPNTNGVQIHNDIKPLDTETVIVKHYPNSFYETNLQNKLVEIE
                     VTKLVVCGMMTHMCIDTTIRAAKDYGYEITLISNGCATKELKWNGVNIPADTVQSIFM
                     ASLNQKFANVITSTEFLSNEITR"
     gene            1245..1610
                     /locus_tag="KPK24_003675"
     CDS             1245..1610
                     /locus_tag="KPK24_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301305.1"
                     /translation="MKLRDEYTCPLELIHDMIKGKWKPIILWRLRLGATSLSKLENDI
                     DGITQKMLLEHLKELIDYGFVDKKTFSGYPLHVEYFLTEDMGNQILEALRIMQHIGIE
                     YLRKNGMENELTEKHVIPD"
     gene            1698..2198
                     /locus_tag="KPK24_003676"
     CDS             1698..2198
                     /locus_tag="KPK24_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG2301306.1"
                     /translation="MYISSIGISNGIIEKKYGSHGVQFNENDVPTYSIPFKIENAPDK
                     TVSFAFILEDKDAYPVTGFIWVHWLGANLTRSELKENESQTAKDFIQGANSWTSILGN
                     QQTREQTSCYGGMAPPDKAHIYELHVFALDKLLDLKNGFYLNELYQEMEGHILEEAVL
                     KGLYEK"
     gene            2188..2703
                     /locus_tag="KPK24_003677"
     CDS             2188..2703
                     /locus_tag="KPK24_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG2301307.1"
                     /translation="MKSKPVKVGLRTIKTVLSAFLCFLIDSLRASSVPFYAVIASILC
                     IQPDMENSLSVAKNRELSTIIGGILGMLFLLMERIFLPIQPEILRELTLSILLIPIIN
                     LSVLLKQKKGTFLTCVVFLCVTVTHEKDISPIWFAWNRIVDTSLGIGISLAVNSFPFH
                     KFKVGGTMGKY"
     gene            2697..2978
                     /locus_tag="KPK24_003678"
     CDS             2697..2978
                     /locus_tag="KPK24_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2301308.1"
                     /translation="MLIVEFTEKEDAVFQQAVEWLSSKLAYTPASFNQQKKGFDIDPL
                     TRTFTNEEGTSIYLTAKEFDLLYFLSSHKGQVFTKEQIYEKVWGYDNFY"
     gene            3132..3322
                     /locus_tag="KPK24_003679"
                     /pseudo
     CDS             3132..3322
                     /locus_tag="KPK24_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            3346..3570
                     /locus_tag="KPK24_003680"
     CDS             3346..3570
                     /locus_tag="KPK24_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301309.1"
                     /translation="MIGIKKRDIKKALKAGAKAGGSSYADVLADIEATIDEVMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTRV"
     gene            3592..>3686
                     /locus_tag="KPK24_003681"
     CDS             3592..>3686
                     /locus_tag="KPK24_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2301310.1"
                     /translation="MKNEMWVLCPICNNKTRTKVREDTELINFPL"
BASE COUNT         1329 a          501 c          721 g         1135 t
ORIGIN      
        1 ttccccatag aataccttat cgcctaaatc attacgagaa agcccagctt tttcacgggc
       61 ttctctgatt gccagcccta gcggtctgaa atcaaaagta tgtgtttctt ttgtttttct
      121 cattttaaat caccatacat gagtttatac ttcatggttc gtatttagaa tgtgttaaaa
      181 gtgcatatat tagggtaata aaatatgttt tgaaatattg agcttaaaca tgtaagatat
      241 ttcacatagg agatgataaa atgaaactta gagaaagcta tacatgtcca ttagaacttg
      301 ttcatgatat tataaaggga aaatggaaaa cgataattat ttttcaactt agaaatggaa
      361 aatgctcttt ttccgaactc catcacagta tagcaggaat tactcaaaaa atgctattgg
      421 aacaactgcg tgaattaaaa cactttggct taattgataa aaaaacatat gatggatatc
      481 ctctaaaagt agaatacttt ttaactggac agggagaaaa gatgctatca gcaattaaga
      541 taatgcaaga aattggtata gattacatgg tagagcatgg tatgacagaa atccttgatg
      601 aaaaaggaat ttgttattaa actctatcat atacacgaaa aagtaagtaa tttacttaga
      661 aactattatc ttatattatt taattagaat taagagagga tatgagagta tgaaaaaagc
      721 attacttatt attgatgtgc agaatgatta ttttcctaat ggaaagtgcc agttgtataa
      781 accagagatt gcgttgaata caataaaaga tttattgaaa atttttagaa agcaaaactt
      841 gccaattatt tacattcgcc atgagtctat ccatggaaca ttttttaatc caaatacaaa
      901 tggtgtacaa atccataatg atattaaacc attagataca gaaacagtta ttgttaaaca
      961 ttatccaaat agtttttatg aaacgaattt gcaaaacaaa ctggtagaga ttgaggtaac
     1021 gaaattggtt gtttgtggaa tgatgacaca tatgtgtatt gacacaacta taagagctgc
     1081 caaagattat ggctatgaga ttacactaat atcaaatggt tgtgcgacca aggaattaaa
     1141 atggaatgga gtcaatatcc cagcagatac agttcaaagc atatttatgg cttctttaaa
     1201 tcagaaattt gctaatgtta ttacaagcac ggagtttttg tcaaatgaaa ttacgagatg
     1261 aatatacatg tcctttggaa ttaattcatg atatgataaa gggaaaatgg aagccaatta
     1321 ttttatggcg cttacgatta ggtgctactt cattatctaa attagagaat gatattgatg
     1381 gaattacaca aaaaatgctt ttggaacatt taaaggaact tatagattat ggatttgttg
     1441 ataaaaaaac attttctggt tatccacttc atgtcgagta ttttttgacg gaagatatgg
     1501 gaaatcaaat actagaagca ttacgaatta tgcaacatat agggattgaa tatttaagga
     1561 aaaatggaat ggaaaatgaa ttaactgaga aacacgtgat tccagactaa tacctacaaa
     1621 aaagtgggta atttacgttt tgcttcctct ttgttaatat atatctgtaa ttaaacgata
     1681 tacaaggagg aaaatttatg tatatttcaa gtataggaat ttctaatgga attattgaaa
     1741 aaaaatatgg tagtcatgga gtgcaattca atgaaaatga tgtgccaacg tattcaattc
     1801 cgttcaaaat tgaaaatgca ccagataaaa cggtttcctt tgcctttata ctggaagata
     1861 aagacgcata tccagtgacg ggttttatat gggtgcattg gttaggagct aatctaacaa
     1921 gaagtgaatt aaaggaaaac gaaagccaga cagcaaagga ctttatacaa ggtgcgaata
     1981 gctggacaag catattgggg aatcagcaaa ccagagaaca gacaagctgt tatggtggaa
     2041 tggcaccacc agataaagca catatttatg agcttcatgt atttgccctt gataaactgc
     2101 ttgatttaaa aaacggattt tatcttaatg aactctatca agaaatggag gggcacatcc
     2161 ttgaagaagc tgtactaaaa gggttgtatg aaaagtaagc ctgttaaagt tggattgcgg
     2221 acaattaaga cggtactttc tgcgttcctt tgttttttga tagatagctt aagagcttca
     2281 agtgttcctt tttatgcagt gattgcgtct atactgtgca tacagccaga tatggaaaac
     2341 agtttgagcg ttgctaaaaa cagggaactt tcaacaatta tcggcggtat actgggaatg
     2401 ctctttttgc ttatggaaag aatttttttg cccattcagc cagaaatact tcgggaattg
     2461 actttatcta ttttattgat tccaatcatc aatctgtctg tactgttgaa acagaaaaaa
     2521 ggaacatttt taacgtgcgt tgtattctta tgcgtaacag taacacatga gaaagatata
     2581 agtcctattt ggttcgcatg gaataggatt gttgacactt cattagggat tggtatttca
     2641 ctggcagtaa actctttccc ctttcataaa tttaaggtag gtggtactat gggaaaatac
     2701 tgattgttga atttactgaa aaagaggacg ctgttttcca acaagcggtg gaatggctgt
     2761 cctctaaact tgcttacacc cctgcttctt ttaatcagca gaaaaaagga tttgatatag
     2821 acccattaac ccgtaccttt accaacgaag agggaacgtc tatatatctg actgctaaag
     2881 aatttgacct actatatttc ctttccagcc acaaaggaca ggtgttcaca aaagaacaga
     2941 tttatgaaaa ggtatgggga tatgacaatt tttattagtc tggcaataat gaagaaaaaa
     3001 gaattttaga aaagtgaggt gtgcttatgg atttatctat tttggtagta gtattgatat
     3061 tgggtgtggg tattccttta agaaataagg caatcaaaaa atacagataa aatatgaaaa
     3121 agaaaggaaa aatgaaaaaa tatgaatatt tagaaattga ttatactgcg aaaggtgttg
     3181 tattcctttg caccgataaa cataaggaag ttataaatag ctatgcagaa aatgggtatc
     3241 gctatgcggg ttcgtgccta cagaaattga cgcaaaaggc tgtatgcgaa agatagattt
     3301 aatttttgaa aaagaagatt aacgtatttt agggagttgg ttcttgtgat aggaattaaa
     3361 aaacgagata ttaaaaaagc attgaaagct ggtgcaaagg ctggtggttc ttcgtatgct
     3421 gatgttttgg ctgatattga agcaacgatt gatgaagtta tgaacagcac cgacccagaa
     3481 gtgcaggcaa atttcaaaaa gtattttgga aataaacgcc ctacaccaga ggaatatatc
     3541 tataaaatca cgaagaaaac acgagtttaa agaattagta agggtggttc tgtgaaaaat
     3601 gaaatgtggg tattatgtcc tatttgcaat aataagacac gaactaaagt gcgagaagat
     3661 acggaattga taaattttcc tttgtt
//
LOCUS       DAEMGG010000214         3560 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEMGG010000214 DAEMGG010000000
VERSION     DAEMGG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.217"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..717
                     /locus_tag="KPK24_003682"
     CDS             <1..717
                     /locus_tag="KPK24_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2301311.1"
                     /translation="DSYKKIFFKDFEIEKINLHIKDANEYDINLFKVQKSILEPEINS
                     QATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYPF
                     NFTSSIESYDNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLIK
                     LGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIYF
                     IGYFIYYFLTLKYALNMIKE"
     gene            942..2555
                     /gene="ggt"
                     /locus_tag="KPK24_003683"
     CDS             942..2555
                     /gene="ggt"
                     /locus_tag="KPK24_003683"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG2301312.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2688..3539)
                     /locus_tag="KPK24_003684"
     CDS             complement(2688..3539)
                     /locus_tag="KPK24_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG2301313.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNGLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
BASE COUNT         1361 a          456 c          577 g         1166 t
ORIGIN      
        1 gattcataca aaaaaatatt ttttaaagac tttgagattg aaaaaataaa cttgcatatt
       61 aaagatgcaa atgagtatga tataaatctg tttaaagtac aaaaaagcat cttggagcca
      121 gaaataaatt cacaagctac gtttgattta attgtagtaa aagatgacgt atttaatcag
      181 ttaaaattac atggaaattc tagattaata agattaattg atataaaaaa tgaaaaaaaa
      241 gaactggatt tgactttaaa attaaaaaat atagtaagtg aaaatatgaa atttacttat
      301 ccttttaatt ttacatcaag tattgaaagt tatgataact tgataaaatt aagtggtcta
      361 atgctgttta taggcatatt tttgtctgta gtatttcttt tatgtacagg tagtataatc
      421 ttatttaaac aattatcaaa tatatatgat gataaggaaa gatatattat gttaataaaa
      481 cttggagcaa ataataaaga tattgagaaa ataatatcaa aacagttaaa agttatattt
      541 ttaatgccat tagttgtagg tacagttcat aatttatttg ctatgtctat agcacaaaag
      601 tttataccaa ggtcattact tgtaccaatt atcataactc tagtcattta ttttatagga
      661 tattttatat attatttttt aactttaaaa tatgctctaa acatgataaa agaataaatt
      721 atttttaagt taaaagaatt agatatatgt tttgaataaa ataacttatt taagtgcaga
      781 tagaaaatac tagaattgag tttaagatat ttatttaaag ataaaattaa agttaggaaa
      841 aagtctatat tttatatcat tttgtatcag ttacattaat tgatgttata taataaatct
      901 aagcagaata tcaaataaaa aatgacgagg agctgaaatt tatgaagttt aatgcctacc
      961 aacataaata ctcatcaata agaaatgtcg tgtatgctaa aaatggagca gttgcaacat
     1021 caacaccatt agcatctcaa gctggacttg aaatactaaa aaagggtgga aatgctgttg
     1081 atgcagcagt tgcaacagcc gccacattag cagtagtaga acctacaagc aatgggattg
     1141 gaggagatgc atatgccctt gtttggatag aggaagaaaa acgattgtat ggtttaaatt
     1201 ctagtggctt tgctccagaa aatatggaat taaaaaatta taataatatg aaagaaatgc
     1261 caaaatatgg gtttggagca gtaactgtac ctggaatacc agcagcttgg tcagaattaa
     1321 ataaaaaata tggtaaactt agcttaatgg aatgcttatc tccagcgata aactatgcaa
     1381 gagagggata cgtagtatct cctaatgtag ctaaggtatg gaaaaaatca tatgaactct
     1441 atgaaaaaga acttattgga gaagagttca aaccatggtt tgatacattt tctaaagatg
     1501 gtaaagctcc agaggctgga gatatattta tatgtgaaga acaggcaagt acattagaag
     1561 agatagcaaa tactcaggct gaaagctttt atagaggaag acttgctgat aaaatagatg
     1621 aatattctaa aaaatttaat ggggctataa gaaagagtga cttagagtgt ttttatccaa
     1681 catgggtaga acctataagt acagaatata agggatataa aatatttgaa atcccaccaa
     1741 atggtcatgg aataacagtt ttaatggcac ttaacatatt aaaagaattg gaattagaag
     1801 gaaatataga aaatgtagaa gacatacata aaattataga gagtttaaaa ttagcttttg
     1861 cagattctaa aacatatgta actgatatag agcatatgaa ggtaaagatt caggaattac
     1921 taagccaaga gtatgctaaa aaaagaagtt tattgattga caataaagag gctttgtatc
     1981 caactgcagg agagccatat tgtggtggta cagtatacct ttgtactgca gataaggatg
     2041 gaaatatggt ttcatatata cagagcaatt acataaattt tggttcagga attgtgatac
     2101 caagaactgg gattgcttta catagtagag gaaataattt taatttagac cctaaacacc
     2161 acaatgtagt aaaaccattt aaaaaacctt atcatactat aatacctgga tttttaggta
     2221 aagaagataa agcaataggt ccatttggtg taatgggagc ttttatgcaa ccacaaggac
     2281 atattcaagt gttaacaaat atgattgatt ttggattaaa tccacaagag gcgctagatg
     2341 ctccaagatg gcagtggata aaaggaaagg aaatagaagt tgaaccagag atgccaaagc
     2401 acataataga ttcattaatt gaaaagggtc atgaaataaa agtaattcat gatactgtcg
     2461 acatgggacg aggtcaaata atatttaaaa cagaacagga aagttatatt tgtgggacgg
     2521 aatcaagatg tgatggtcat attgcagttt attaatatta tcaactaaaa tatatataat
     2581 aaatttaaag agtatatgaa ataattagta aaggcgaaag tctacaatga ctgacgcctt
     2641 tacaaaattt aagctatcat atgctgattt tatatagatt atgagcctta ttcttgacca
     2701 gttttgattt cattgtcatc ataactaatc cagtcactaa agctaccaat ataaatcttg
     2761 tgaggtatat ccagttctct aagagcaaga ctgttaacac aagcagatat ccctgaacca
     2821 caagataaaa ttacttcatc ataattattt aaatctttaa agaatttttt taaaaattct
     2881 atatctttaa gtgagccatt ttcaaagtca ctttttatta aatccttaca aaaataattt
     2941 tttgcacttg gtatatgacc agcttttgag taagcaggtt caactaaacc ctggtatctc
     3001 tcattagcac ggcaatctat tattacagta tcttttttgt ataatttaga cttaacatat
     3061 tccattggaa caactaaacc attgtttata tttggtctaa tttccttacc tgtacaactt
     3121 ggtatattca ctttctcttc caattctcca ccttccttta cgaaagatgt tattccccca
     3181 tccagtacat agacattttt aagccccaaa tgttttaatt ggaaaaataa tctgcaagct
     3241 ccatttaaat ctccatcatc ataagttaca atcgtagtgt cgttatcaac acccattttt
     3301 tctaattttt cttttagtat taaagggtct tgtaaaggat ttttaccacc atgttctttt
     3361 gtaggagatg ataaatcctt gtctatatct aagataaagc tgcctttgat gtgtcctttt
     3421 ttataagaat caataccata agttctattg ataagatcaa atctgcaatc tattacaact
     3481 agattatcat ttttttctag ttttgatata agttcttttg ctgaaataat gtttttcaaa
     3541 gaatgtacct cctgtgttta
//
LOCUS       DAEMGG010000215         3502 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEMGG010000215 DAEMGG010000000
VERSION     DAEMGG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.218"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            44..1129
                     /locus_tag="KPK24_003685"
     CDS             44..1129
                     /locus_tag="KPK24_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG2301314.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGFVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            1183..2307
                     /gene="wecB"
                     /locus_tag="KPK24_003686"
     CDS             1183..2307
                     /gene="wecB"
                     /locus_tag="KPK24_003686"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG2301315.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGMQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2385..3359
                     /locus_tag="KPK24_003687"
     CDS             2385..3359
                     /locus_tag="KPK24_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG2301316.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1470 a          336 c          606 g         1090 t
ORIGIN      
        1 aagataatat gataaattta ttattttaaa agaggtatat aatttgagga aatatatgaa
       61 taaatttaaa ttaaaaagaa gtattttaat ctcattaata atcttaattt ttatatttat
      121 agtttacttt gtaagtattg agttaaaaga taggagtcta gtaaataatc aatataaagg
      181 taagattaaa tcggccaatt tatctgtaga ttatgaaata aatcaagtta tgaaggatat
      241 agataaattg ggattgaata cagtaaatgt acctataatt ataaatgtag agtccattaa
      301 ttctgatatt atgtcaatag ataataatag taaagaaaag gcaattaagt taataaaaaa
      361 attaaataaa aaaggtattt caacaatatt agaggcatat ccatggattg aaaatggaaa
      421 attgtatgag actgactgga accctataaa taaaaaaaga tttttttata cttggcaaaa
      481 tgccatatta aatgaactta taattgatgt agcaaatcca ctggatgtaa atgttttaaa
      541 tattggttct aattttgtac atttggaaga atatcaacaa aattggggtg aaataataga
      601 ttttgtacaa tccaaatttg acggttttgt aacgtacaga actaattggt ggtatacaaa
      661 aaaagatgat ttaagtagta agttgtttta tgaacaaaag ctgaacaata atttttttga
      721 taagttagat tttatatcta tagcagcata ttttgagtta agtaataaac ctgttaatac
      781 agtggatgaa ttaatttctg ccttacactc atctactgta aacaatagag gacaaaatat
      841 aaaacaagaa atatataacc tttataaagc tcatagaaaa cctatatttt ttggagaatt
      901 aggtttttca aatagagaaa gtgcaagttc tcagccgtgg aatcatactc cttcaaaagc
      961 tgtaaatggc gaagaacaag caaggtgttt tgaggcatat aagaaagttt ttgaaaatga
     1021 agactggata aatggttttt ctgtgttttg tgtaggtaaa atagatgacg aaaaaaattt
     1081 ttacccatca aaagagagta taaaagttat taagtcttgg tatgaataat tttgaaagtg
     1141 tagaatttat atagattaaa tagatgatta ggagagacat aaatgaatga catcaaggtt
     1201 atgactgtat ttggaactag acctgaagct atcaaagtag cacctttaat aaaggaactt
     1261 gaaaaaagag agaatataaa aagtattgtt tgcgttacag ctcaacatag agaaatgctt
     1321 gaccaagtta tagaaacctt taatataaat gtagactatg atttggatat aatggaaaaa
     1381 ggacaaagcc tgaatgatat aacatgtaag attttaaata aacttccctt gattttaaac
     1441 aaggaaaatc caaatataat attggtacat ggagatacaa ccacaactct agctacaagt
     1501 ttgactgcat tttataataa aactcttgtt ggtcatatag aagctgggct tagaacctat
     1561 gataaatatt ctccttttcc agaggagtta aatagacaac ttacaggtat tattgctgat
     1621 atgcactttg ctcctactaa tttggctaga aaaaacttga tttctgaagg aaaacctaat
     1681 aataatatat ttgtaactgg taatactgct attgatgctt taaagatgac aataaaagaa
     1741 aactataatc atcctataat tgatgagatt ggtaacgata ggatgatttt actaacctct
     1801 cacagaagag aaaatttggg taaacctatg aaaaatatat ttagagctat aaagcgaata
     1861 gtagatgact ttgaagatgt acagatagta tatcccatac atttaaatcc taaaatacga
     1921 actatagctg atgagatatt tggaaaattt cctgaaaaaa ttcatattat agagccttta
     1981 gatgtagctg attttcataa ctttttaaat aaatcttata tgataatgac tgatagtgga
     2041 ggaatgcagg aagaagctcc atcattaggt aagccagtcc ttgtgttgcg tgataaaaca
     2101 gaaagaacag aaggaattga agcaaagaca cttaaattag taggtactaa tgaagataga
     2161 atatataatt cagtgagcga tttattaata aataaagata actatgttca gatgagtaaa
     2221 gcatctaatc catatggaga tggaaatgca agcaaatata tagtggatat aataattaaa
     2281 aaatttaatt gcaaatattt aaattagata agacctaaaa cattaactaa ttgatataag
     2341 atttatgata atattaagga atacaatttt taagggggag taatatggct agaaaattaa
     2401 taaaaaattt gggtaaaagt aaaagtgtaa agagagtgaa acttttattt aagaagattt
     2461 ttattactgt attcatagta gcaagtatag ttgctatttt taatataacg aaatattttg
     2521 aggaattata caaagtaagg gatttaaaaa gtactaaaat tgaatactat atggatgtgg
     2581 ctgatgaagc aggagatgga aaggtccaat taagttggaa ggccttactt gctatagaca
     2641 tggtaataca tgatgaagat ttaagtaata taaaaaagaa agacacattg gatatagggg
     2701 aaaagtttat agtagaagat aaaaatgata aaggcgaaaa ggtgtataag gtaaaaaagt
     2761 ttaataaggt attaagcgaa ttgaaatttg actcttctca aaaaagtaga gcaagaaaat
     2821 acatgaaaga tttagaatac acataccttg gaaataaaca attagatagt agtgatgaaa
     2881 aaattaaatt tataaagaag ttagaagact cagctataag agaatatatt gattatggaa
     2941 tattgccctc tataacaatt ggacaagcta tattagaatc tggttgggga aattctaaac
     3001 ttacaaaaca gagtaataat ttatttggta taaaagcaga taaagcatgg aaaggaaaaa
     3061 gtgtagaaat ttcaacttca gagcattata atgaaaaaat tgtagctagt tttaggtcgt
     3121 ataattcatt acaagattct gtcaaagacc acagtttatt tttaattaat aataaaagat
     3181 atagaaaaca tggattgttt gaggcaaaag attatattag tcaagctcaa tcattagaaa
     3241 atgcaggata tagtacagca gaagataaaa aaggaaatcg tatatatgca gagctattaa
     3301 tagatgttat taggagttat aatttgcaat tgatagataa taaagttgag acaaaataat
     3361 tgttaacttt atatgaggag ataacaagat aagattcttt tctacaaaaa gaatagaaaa
     3421 attgtaaaaa aaactaaaaa aatgtattga cttgttattt aaaaggtgat attattatta
     3481 ctgtactcga aagagaacaa aa
//
LOCUS       DAEMGG010000216         3490 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEMGG010000216 DAEMGG010000000
VERSION     DAEMGG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.219"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(563..1315)
                     /locus_tag="KPK24_003688"
     CDS             complement(563..1315)
                     /locus_tag="KPK24_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG2301317.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VAMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(1374..3029)
                     /locus_tag="KPK24_003689"
     CDS             complement(1374..3029)
                     /locus_tag="KPK24_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2301318.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILRLRISVYDLKYKEI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIYIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSVSDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
BASE COUNT         1222 a          554 c          286 g         1428 t
ORIGIN      
        1 ttattattat aataacaaaa gaataaaaaa aaattaattt agcatagcct tgttaaatat
       61 agatttgaat atgaatttaa agttatgtaa aattaaaaat acaggaatga aaattccatt
      121 cctgtataaa atctaacttt tgagtctcag tacaatctta ttttgagaca atcttttttg
      181 ttttatattt tcttatattt aaaatataac gcatatctta tgcaacatat tcatattttt
      241 ctttatattc tttcatcgca tcattccaag cgtcttgtac tgttacacct cttttattag
      301 caattttttg tgccagttca attatcttca tagctcttct tgatatgttc ataaacattc
      361 ctcctacaaa ttaccatata tttcatatat ataaatcaag gttatatttt tatgatatat
      421 aatccttcta ttgtaagatt acagtttata tgtgtattat atatgaacta tattagaaga
      481 taattagaat ttttataaag ctatatgatt agattaaata taaaagcttt acaattactt
      541 ataaatttaa aaagtctata aactaaacta atgtttgata taccaatttt acgaccataa
      601 ataatgccat tactaaaaat attggtttta tcatctttga acctttcttt atagcaactt
      661 tagcacctac ataagctccc attatcatca ctaatgcaaa cactatagca attttataat
      721 ctacttgacc atttatcatg aataataaca atgaagttat attacttgtt aagtttaata
      781 tttttgtatt tgcagaagca tgtaaaaaat catatccata tatttttata aatccaaaag
      841 tcaaaaaagt tcctgtacct ggaccaaaaa aaccatcata aaaacccatt ataagagcca
      901 taagcatccc tagtcttaaa tttttcttat taacagcttc aaaattatct tcttgaccta
      961 aatttttaga aataaaggtg taaatagcca ctattaaaat catagccaca accaaaacat
     1021 ttaatatctc ttcacttatt gataatacac atctcacacc tagaacacta ccaataatag
     1081 taaatggtac taattttttt aaaaggttgt tattaatttt tccagacttt gcatatctat
     1141 aggcactact tatacatcct gctgatgctg caaatttatt tgtaccaatg gcaagatgta
     1201 ctggcactcc aattgccata agaacaggca tgcttataag tcctcctcct cctgctatag
     1261 aatctacaaa agcagctaaa aaccccccta tacataaaaa aataactgaa aacataaaat
     1321 tcctccatca ttctctttca atttttctta caatatatat tatataatct tatttaatac
     1381 ttattatcca gcatattctt tctatattca tttggagtta tactattaaa cttcttaaat
     1441 atttgtgaaa aataactact gttattaaaa cctactttct tacaaatatc actaacactc
     1501 atgcttctat ctaacagata ttttttagat tcttctatcc taagaagatt tatatactca
     1561 gttattgaaa ctttatttct ttcattaaaa atagaactca aataatttgg acttataaaa
     1621 aaaacatctg atatactatt caaatttata ttttctttat aatatttttt tatatattcg
     1681 tttatatttt caattagatt tcctttatct tttctccaaa atgctctatt cttatcaata
     1741 cactctttgg aaaattcaaa taatacattc cataaatctt catcactgtt tacaagtgat
     1801 atttttttat tatatttatt tcttaaataa gatataagtt ttaaatcctc taaatctcta
     1861 agcactgttc tagacaatac aaatatcatt tcctgaatag tatctctaac atcattatat
     1921 cttaatttgt ttgtatctat tagattataa acaccttcta aattcatatt tttaatttct
     1981 ttttcaatat aaagttctaa ttcaaaggga tattcttttg aagaattatt gaacatatct
     2041 atcggattaa taatatactc ttcttctaat aatctactat ttgctttttt aagtgcctct
     2101 tcaagtctta tattttcttt tctactcttc aatatctcct catcttttat actaagttga
     2161 ttttcaaaaa aattcctctt agctatttcc tgaataaatt cagacatcat taataaattt
     2221 tcttttaata catataatct acttttagga tttaatggaa attcattgta aattttgttt
     2281 atgtatttta tatcaatacc agaactactt gataattcag ataaattttt atctatataa
     2341 atagacttat atactataaa attaccacat tctatatatc caactatatt ttcttctaat
     2401 actataggta tctctaaact ggaaacacca tactcacaat gatacacttt ttcaaagtta
     2461 tcattattta tgcttttaac aagtttttta gtacacggtt ttatattttt acaaatatcg
     2521 caaaatttat tttctttctt agtctcaaat atttctttat actttaagtc atatacagat
     2581 attctaagcc tcaatatatt agatatttga tttgcaagat tttcaattat gtttacatta
     2641 actaactcct gtatattgtt ttcagaaaat actataggta tttgtctcaa actcgaaaat
     2701 ttatgaggaa cataagtaat taatattact aaatcaattt taccagtgtt tataatttca
     2761 tgttctgaat atggtgggca ataaactata gatgtttcct ttatattgac ctttttacca
     2821 tcaattattt gtattccttc tccctgaatc acatataaaa gctgttcttc acttaaatga
     2881 aaatgctttt cttggcagtt tcccggaaaa aatcttacaa ttccagcact aagtctattg
     2941 aattgtgttt ttgatggttc gtgtatccac ataacctctc cccaatcaaa tttttgtact
     3001 tcattatttg actcaataaa atccaccata aactcacctc gtagtttttg tctaaatatt
     3061 ttttcttccc ttcttatatt aaaataatat catcatcatt aactttcatt actataaaat
     3121 catcatactc ttttaatact cctgtaattt tagttgcact tagtttacta caattcagtt
     3181 ttttaatata ttgataaatt ctataattac ttttttattt tgtaaaatca gtgttttccc
     3241 aaaataatta tatctataaa tgaaagtatt tttactgctt acatcaatat atgttatcat
     3301 aattttcctt tttattgcat atataaataa ctgtatataa cattgtccaa tactatatag
     3361 aaatacatct aagaaagaat attattaaat tgttccaatt aatattattt ttcttataga
     3421 aataaaaaac tgttggattc ttttagaaaa attatttttt acataccttt tttgcttaaa
     3481 tagtctatct
//
LOCUS       DAEMGG010000217         3484 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEMGG010000217 DAEMGG010000000
VERSION     DAEMGG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.220"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..895
                     /locus_tag="KPK24_003690"
     CDS             23..895
                     /locus_tag="KPK24_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2301319.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            892..2181
                     /locus_tag="KPK24_003691"
     CDS             892..2181
                     /locus_tag="KPK24_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2301320.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2111..2755
                     /locus_tag="KPK24_003692"
     CDS             2111..2755
                     /locus_tag="KPK24_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2301321.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3022..3484)
                     /locus_tag="KPK24_003693"
                     /pseudo
     CDS             complement(3022..3484)
                     /locus_tag="KPK24_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1301 a          412 c          560 g         1211 t
ORIGIN      
        1 gtttctgggg gtgtatttat atatggaaat gaaaatagca gtatccaatc ttagtaaaag
       61 atttgggaaa aaacaagctc ttgataatat aaatcttgaa atcgacagtg gtatgtttgg
      121 tcttttagga agaaatggag caggaaaaac cacatttatg agaattttag ccactttatt
      181 atcaaaaaca gaaggttcaa tacatatgtg tggagttcct attgagaata ctaaggaaat
      241 tagaaatatg gttggttatt tgccacaaga cttttcaatg tatcctaaca tgacagtata
      301 taaagcaatg gattatttag cagttttatc ggatatacca ctaagaaaaa gaagaaaaat
      361 tatagcaaag ttattaagca aagtaaacct tactaattat caaaatgtga aagtaaaatc
      421 tttatctgga ggtatgaata gaaggcttgg gatagcacaa gcattaattc ataatcccag
      481 agttttaata gtagatgagc cgacagcagg tcttgaccca gaagaacgta taagatttag
      541 aaatttacta tgcgaaattg caaatgatag aatcgttatt ttatctacac atattgttgg
      601 agatatagaa gctacatgtg aaaacgtagc tatattgaat agtggaaaag tcatatacaa
      661 tggctcaaca gaagcattag caaaactagc tgaagggaaa gtatatagta ttgaagtaga
      721 taaaaaagat attgaaaata taaaaagtag atttatagtt ataggaatgt tgactcatgg
      781 tggaaaggct atacttagga ttatatcaga tgataaacct tttgagacag cagtaaattg
      841 taatccaact attgaagatg gctatatgct tataatggga ggagataata tatgaatcta
      901 tttataaaag aatgtaaaaa tatattaaag agtatgatat attatatttt tattgtagtt
      961 ttagtttttt tctatatttc tcaacttgga agttcagttt caaatgatgt aagtaaaaat
     1021 attcctaatg atggaaatcc attagttgct ccacaaaaag tagagaatat tgaggaatat
     1081 tactctgtac acaatcaata tccatatggt caaaaagaat ctatgaaaac agaaaaagtc
     1141 attccaaatg caactataaa cctaattaga gaatatcaaa aaaacacata tacaacgtat
     1201 ccaacagggg tagttaagta tataaaatta gaccaaaata aaacgaagac catagaaaaa
     1261 tatatagaag aaataactgg taaaagtatt agcgaaataa attcactttg gaaaactgat
     1321 gaaagtataa aaatagatta ttctaaagta tctcttgaaa gatttgaaga aataatgcaa
     1381 agagtagacg acattttagg aggagggtct gcatatgctt ttgacaagtt acaaagattt
     1441 ggagtagttc cagttaccta tgatgaagct atgaagaatt acaactatac aatacaaaaa
     1501 gataaagtta gtaatgggta tgcaagaatt ttttgcgatt atataggtat agttttaagt
     1561 atatttccag tatttgtggc agtttttatg tcacttaaag ataagcgttc aagaatgaga
     1621 gagattattt attcaaaaca agtatcttca tgcaaaatag ttttatcaag atattttgca
     1681 cttgtcttta tgatgatatt gcctgtatta attttaggta tgaaagattt acttcctttg
     1741 attttctttg ctaaagataa aggtatatct attgataact tagcatttat aaaatataca
     1801 ttatggtgga tactaccaac tcttatgata gttacagctg taggaatgag tattactata
     1861 ttaacagata caccagtagc aattattgtg caattaattt ggtggcttta cagtataaat
     1921 tctacaggac tcaaaggtga ttattcaaca atggggctaa tgataagaca taatagtgta
     1981 ttaaatgggg atattattat acaaaacttc aatcaaatta tgattaatag aattgtgata
     2041 agtttgggag cattggtgct tgttttgtta gctatattct tatatgagca aaaaagaagg
     2101 gggaatcttg atgtttgggc aaagttttca aaactgttta gttctaataa aagaaaactt
     2161 caaattaagc actttaacta atttaatatg ttcaattata ttgctatgta tgattcctct
     2221 aatacaagga gtagctaatt tagacagaat ttcaacagct atatgtttag aaaattatgt
     2281 tgcaataata gggatagtaa tgttggtacc tgtattttcg ctagagcaat caaaagaaat
     2341 tgatgagata gtatcttcaa aagcattatc tcaagttatt gtacaattaa ttaggatact
     2401 gatgtcaatt ataggtatag tggtattaat cataggtttt gtaaatgttt taatatatct
     2461 tggatgtaat tttccagtag ttgagtatat tattggaagt ttcgcatcag ctatgtttat
     2521 aggaagttta ggaatgtttt tctcaaattt atttaataat acaatcatag gttatatgat
     2581 atctatagga tatttaattt taaatatgat gactaaaggc aagtatgttg gaaaattttt
     2641 tataatgtct atgagtcaag gtagttttga tgagaaatat tggctgatac ttggaagtgt
     2701 tgtgttgata attgcatctc tcttaataaa accagtaaaa aggaagatta gataataaaa
     2761 tgagagctta gataaaataa aggtaatgca aataaaaagt acatttatga aatataaatc
     2821 tatttttata tacacaaacc tacttacaat ctctttatgt atgtagatag atttatatat
     2881 ccgtagtata aaatatatat gtataccgaa ttttatcaaa agctctcaaa tttatatttc
     2941 aaagacactt attgctatga cctacacata tgtatttttt cattttaatt gttgtattaa
     3001 caataggact attgttataa actattgata cctcataaca aaaaattctc catctcctaa
     3061 atgcgttttt tgtaccttag tctcaactat tttaaatcct tgagactcat aacattttac
     3121 agctctcatg ttccaggttc ttacatttaa tacaattggt ttagtactat atttatttct
     3181 acattcttgt aatcctaggc taattatttc tttaccaatt cccattccac aataattcgg
     3241 tttcaaccaa taccaaagaa tacagagctt ccttcatcta ataagtttat aaaacctatc
     3301 aattctttat tgtcatttat aaaacttata aaattttttc ttttatcttc ttttgctagt
     3361 gaaaaattct tctttttaat ttcctcccat ggaggtagat tgtatattga atatttacct
     3421 tcatatttcc aattagtaac ttcaatagca tgttcttcag taaatttaac tttatttaaa
     3481 ttca
//
LOCUS       DAEMGG010000218         3429 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEMGG010000218 DAEMGG010000000
VERSION     DAEMGG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.221"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(202..585)
                     /locus_tag="KPK24_003694"
                     /pseudo
     CDS             complement(202..585)
                     /locus_tag="KPK24_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      811..908
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            963..1139
                     /locus_tag="KPK24_003695"
     CDS             963..1139
                     /locus_tag="KPK24_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301322.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1365..1565
                     /locus_tag="KPK24_003696"
     CDS             1365..1565
                     /locus_tag="KPK24_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2301323.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1729..1941)
                     /locus_tag="KPK24_003697"
     CDS             complement(1729..1941)
                     /locus_tag="KPK24_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301324.1"
                     /translation="MVCKIFITLKTILPKTQIIVTTHSPHILQIDSKEEMIVLDMAES
                     DNVYKKELKLGEYGVLGWTNEGLYLH"
     gene            2316..2498
                     /locus_tag="KPK24_003698"
     CDS             2316..2498
                     /locus_tag="KPK24_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG2301325.1"
                     /translation="MFKFYNNATLMFLPGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2569..3414
                     /locus_tag="KPK24_003699"
     CDS             2569..3414
                     /locus_tag="KPK24_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301326.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNKDPKYNLNTI
                     KKHLN"
BASE COUNT         1290 a          415 c          429 g         1295 t
ORIGIN      
        1 atctatagtg ttacttatat tttatatata tggctatttt tcaacatttt acaatatata
       61 acatttattt ttttaacttt tgtcagtaaa atgtcagcaa aacactttat ccacaatttt
      121 tatataagtt ttccacaact tatcaccaat attatccaca taaaaaaggt agcacttata
      181 ttattagcta ccttttattt attaatcttt atatttccag ctttttatcc aacttcctat
      241 ttctattatt ttttcttcag ttatttcttc tccatcatgt aattttgaaa ttaattctga
      301 taaaggattg ttatgtaaac ctctaaaaac tctttttccc tctattttga gatattctct
      361 ctcttttatt aatactgtat agtgtacatg cctcccaatt ctctatgata ttaaaaaacc
      421 ttttttggtt aatctagcta aaagtataat tgtagttgta tgcttccaac catatgtttt
      481 ttctatttca tctgcaacat cttttgatat tacagtttta taaccagtat gccatatgca
      541 tttcataatt tttaattctg tatctggtaa ttttttattt agcataatat attccttctt
      601 tcaatttatg tcacttcttc aaatttatct agcttaaatt ataataggag ctttttagaa
      661 caaaaactat atttaatttt taccaggtac tctacgtgtt tagtctgttt ttaagtgtaa
      721 aataatgttc atatctataa atttatttaa ctttcaaaca atcctgttga ataataacta
      781 attctattgt attattttag ttgtagaata aaactaaatc ggcaaaacta gagaaattta
      841 gtgacgcaaa gctataggga ctaagactta tataaatatc ttatgagtta tgtcagccag
      901 ttgccaaaga gatagtgttc tttttgtttt tatgaaagtt ttttaggggg ataataattt
      961 atatgtttgg aaataaaatg gataaatgta cacatgtatt gactgcttat attagcagtt
     1021 cgtatgatta ttgtaatttt ttagatacac agttagatga ttttatatta gagtacggag
     1081 aaaatgtagt agagtcttgt ttacaccaag taatggtatt ggtaagtaag tataattaga
     1141 tagacattga tttctagaaa acccttttta gttttatcta catttatatt aatatatagc
     1201 aatgtaaaac gtttaaatat atttttaata ttaagaataa ttaaaatgtt aactaaaaaa
     1261 tataattaag tagacatttc tattaatcta tgatacaata gtatttgtgg taatacacaa
     1321 ttaaatcaag tatatcttga tataggttaa taggagtatt tttaatgaat aatggaatag
     1381 tgaaatggtt taataatgaa aaaggattcg gttttatatc aatggaaggt agagatgatg
     1441 tgtttgctca tttctcagct atacaaactt caggatttaa atcattagaa gaaggtcaac
     1501 aagtaagctt tgatatagtt aaaggtgcta gaggtcctca agcagaaaat ataactatat
     1561 tataatatat agatttttcg ggaaagactc tttttagagt ctttttttat tgttaatata
     1621 tgtaagataa tcattggtga agtaaataag gtatattgag taatgttctt gatagtttat
     1681 atgctaatga atttcaaaaa aataagcact cctataaaaa gagtgctctt aatgtaaata
     1741 taaaccttca ttggtccagc ctaatacccc gtattctcca agttttaact cttttttata
     1801 tacattatca ctttcagcca tatctaacac aatcatttct tctttagaat caatttgtaa
     1861 aatatgagga ctgtgtgttg tgacaattat ttgtgttttt gggagaattg tttttaatgt
     1921 aataaaaatt ttacatacca catagttgat ataaaactta agactagcag actcataagc
     1981 aattagttgc ctctctttac ataccacata gttgatataa aacctagagg ctctcagaga
     2041 agttataaga tgtttagtaa ctttacatac cacatagttg atataaaact ccaaaataaa
     2101 cttagcatct ccaatgttta cacatataca atgctcttaa aattgcagtg aacgcgcaat
     2161 attgcaattg ataatactta tcatatattc tcaatacctt atatttcaac tgctaaacct
     2221 caatttatca caaatatcgc tcactgcaaa atttctatat ttttattata tcatagcata
     2281 taattaatat tttcaattaa tttttacatt taattatgtt taaattctat aataatgcaa
     2341 ctttaatgtt tcttcctgga actaatataa aaactatttc agaaagatta ggtcatgcag
     2401 atataaaaat aactatgaat agctactctc agtttttaga agagatggac agagaagcat
     2461 ttgaaaattt gagcaaaata ctatttattg tattttaaag aagatgactt attcaatatt
     2521 tgatgctaga atataattaa taaaaattta attataggtg attgtattat gaatattatt
     2581 aaaagcataa ctttactctt aacattatta attgtgatat taatgacagg ctgttcccca
     2641 agagaaagtg ctagtgaagt tgcaaaaaaa tatattgata agatgaactc tggtgactat
     2701 gaaacagcat atagtttatt aagtaaagat agtaaaaaaa aaattagtat agaaaatctt
     2761 gaagaatttc aaaatatttt agttagtaca aaaagaataa ttagttataa aataggtaaa
     2821 gaaaaaatca taaaaaaata taaacatgat ggaaaagaat ataaaaatgt tgttaaatta
     2881 gaagaaactt ttaatataaa aaaccttctt tataaaaaag atgattctct aaaagtaaat
     2941 agatattttg tagttgaaaa taacaaatac aagctattat tgtatgaaag tttcaaaaaa
     3001 gaatttggta ttaatagtat ggaccttgct caacttaagc ttaatgattt tggactaaaa
     3061 tactttaaag aagttgacct tatactaaaa aaagctattg ctattaatcc tactgattca
     3121 aagctttatt atgcccaagc atgtaattat atgaattttg atggatttcc tggttattca
     3181 gatacaacat ataatgcttt agattcaatt aataaatctt taaagtattt agataaaaat
     3241 gattctgact acaaaaaaaa tgcaagtaat atatataact taaaaggagt tattcttatg
     3301 tttcacaaaa gatttgatga agcacaacaa tatttaaata aagccttata ctttaacaaa
     3361 gataataaag acccaaaata taatctaaat acaataaaaa aacatctaaa ctagatattt
     3421 aatttttga
//
LOCUS       DAEMGG010000219         3362 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEMGG010000219 DAEMGG010000000
VERSION     DAEMGG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.222"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(93..458)
                     /locus_tag="KPK24_003700"
                     /pseudo
     CDS             complement(93..458)
                     /locus_tag="KPK24_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(479..1558)
                     /locus_tag="KPK24_003701"
     CDS             complement(479..1558)
                     /locus_tag="KPK24_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG2301327.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            complement(1612..2901)
                     /locus_tag="KPK24_003702"
     CDS             complement(1612..2901)
                     /locus_tag="KPK24_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG2301328.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     misc_binding    complement(3064..3275)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1107 a          678 c          370 g         1207 t
ORIGIN      
        1 tatcctatct ctacatatag gtttccctat atgctgacta ttttagctac taactcatga
       61 ctaaagtcac gagtgtgcgt agctattttt taatcaaact catatccacc agataccaat
      121 attgaatcta attctatttt ttctcttaag tcttctggcc aatctataaa tggaagaatg
      181 gcagtagtgg ctggctttcc tattcttatc atagccacat tattaagtct ttttctttct
      241 acaatagcat ttgtacaatg agttgtacca agcatagcat attttattaa tgaatggtca
      301 atcttacttt catctataac tttttttaag gcattgtaaa caccagtttc aacatctttt
      361 gtagttgcca ccttaactga atgaattact tttaattccc catccagtat acaagcatct
      421 gtatttgttc ctccaacgtc aattcctaca cgatacatca aaatttcctc ctaaactttt
      481 attttccttg taattcttct actgttttat attccacatc atatccaaag tatccaggtc
      541 caactgtctc aattcctttt tctgttctcc atttttcatt acaaggaaaa gctactacca
      601 taacacgaga gccatatttt attctctctg tagttattgg aaatccagtt tctaaatcta
      661 atactgctat taaatctggt gtaatacaaa gtggtgtatc accttttttg gcaattaaat
      721 tctcattttg gaataatata gaatactctc catcatactc attaatccca gcaaaaaagg
      781 catgtcctct agtaaatcca cctttcaact ctctcttaat atcaattact tttccttcaa
      841 ataacttgta tccacgcaat tcttttagta tattctcaac agcacctttt gaatcagatt
      901 tattttcaag taaaaaccct cctatagttt cagctattgt aagagtatta cctatagcag
      961 cttttttaac ttgagctcca cttaaattat agtcactaat agaagaactt cctcccattt
     1021 ctacagttaa agttctagct aatctttctg accaaattcc atccacagga taaaggatgg
     1081 cagaattacc tttttcatca gacattacgg caggagatgc ctcatatcct tcaagataaa
     1141 aagttaccat ttgagcttca gggaaagctc tacccataga atctgcatct aagataggaa
     1201 gtcctttttt agcagcaact gctataggca taagtgagtt tatccctcct atttctatag
     1261 gaattataac atccactttt ctatttaata atttctccat tatttctaaa ggggtagtta
     1321 actcaacttc tgaaagtaat ttttctattg taattacagg agaacccata ccagatactg
     1381 gaactaccaa agcatcatca tcaagttcat ctaaacttat tacttttaca ggaccatact
     1441 ttttaatggc ttgttttgca accaatttac ctacatatgg gtcacctcca cctccagttc
     1501 caagcactgc tgcaccaact gctattttat ctacactttc ttctgttaaa aatctcatgt
     1561 tactttctcc tcatatttct aatttatttt atactttata aacaaatttc tttatactgc
     1621 tttgtctatt tcttgcttat tttttgttga acaaagcact tttccaataa tagattgtac
     1681 tacaaaccct gctataaaac catctaaagg tgctatagta gtaagagtta taaatccata
     1741 tgttgaaagg taagttatta atataccaat tatccaagac actattgaac gtttaactat
     1801 tggtttatat aatactcctt tatcaaagcc tttaagttct tgctttacta tgtaatattc
     1861 tgctgtatat acaccaccaa caggtgcaat tacaattcca agtaaattta aaaatcctat
     1921 aaaaccttca tatattccaa atacagccaa taatgttgct ataataccaa ggactatagt
     1981 taatactttc tcaggagcct tcttaagtac aagagctata cttaatgatg ctgaatatac
     2041 attacttgta tttgtagtcc attgtgctaa tgtaagtact attatacctg gtatagcaat
     2101 tcctaatgta ataaaaattc tcataatatc tgaagttccc atacatttta ctaatactat
     2161 cgaaactatt atcataaaag catttccaaa ttgtattccc aaaaagcttg aaagagcaca
     2221 gtctcttcta cttttagccc aacgacttat atcaggagat acaattgcac caactattat
     2281 tatactcatt gacagagaaa ctccacctgc aaatgtcata gtagatttca tattatcatc
     2341 aactgaaatt ccatttgctc taaaagctaa gaatatagtt agcatcataa gaactaataa
     2401 aaatggtact gaatacacac tcaacttctc cattgcctta tatccataaa ttgcagtact
     2461 catcatcaag agaccaccta ttaggcttaa aatctgcata cttacatcta aattaaatat
     2521 atcttttatt attatctgtg cattttgagc aaagaaacct acttgaactc caaaccaacc
     2581 taatagagaa acccctataa ctagagaaaa tatttttgct ccagcttctc caaatacata
     2641 ctttgttatc atagatgtag ataaacttgt agatgagcca accaaagagc atgtagtata
     2701 caaaattgca gaaaaaaatg aaccaacaat tactgacaat attgcttgtt tcattggcat
     2761 actctgtgcc aactcagcgc ctaaaataac agatgataaa tccacaccta ttgctactaa
     2821 aactgaaaac atagctaccc aaccacgttt ttgattctct ggaatctgtt ccagagcata
     2881 gttaattttt tcttcttcca ttttcatccc tccatttaaa tcgtaaaact actgacctta
     2941 gcatatttta caattttcta atttataaat ttattaacta tcatattttt ataattttca
     3001 tttaatatat agttttaaat tttctgaaat aagtttataa taaaaaacgc ctcttggaaa
     3061 aatccaagag acgaatataa atccgtgtta ccactcttat tagtaaaaat taattttact
     3121 cactcatttt cttaaggcga attaaaccac tattctaaca taattacaat agtattctcc
     3181 aaagtccttt tcacataaat tccaatatta gttctcagcc aaatctaact ctctgtaaaa
     3241 ttcctttatg ctactttctt cttctacgaa tatataatat tgatttgtag ttaatttatg
     3301 atttatatca taatatatat gcaatatttt gtcaacttaa aaagtcaatt acttgcattt
     3361 tt
//
LOCUS       DAEMGG010000220         3344 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEMGG010000220 DAEMGG010000000
VERSION     DAEMGG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.223"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            47..382
                     /locus_tag="KPK24_003703"
     CDS             47..382
                     /locus_tag="KPK24_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301329.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            519..1796
                     /locus_tag="KPK24_003704"
     CDS             519..1796
                     /locus_tag="KPK24_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG2301330.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1917..3305
                     /locus_tag="KPK24_003705"
     CDS             1917..3305
                     /locus_tag="KPK24_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2301331.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
BASE COUNT         1376 a          326 c          588 g         1054 t
ORIGIN      
        1 ataaaagttt gtttaataaa ttaaataaag aaaagaggag aataaaatgt acaacataga
       61 tgatatgata tataattgtc cagtagaagc tttatcaaat attttaggta aaaaatgggt
      121 ggctataatt atatggaaaa tacaagaaga taaaaaacga tttggagagc tacaaagagc
      181 tataccagac tgtagtaaga aaatgttagt tcaacaacta gattttttaa ttgaacaggg
      241 gattatagtc aaccaaaaaa agttggttaa taatgttgta gagtcaacat attttttaag
      301 tgaatcagga ttacgattgc ttcctgtcat ggaaaaaatg attatgtgga gcaataaaaa
      361 tttaaattgt gataaatact aaaatttact ctgtaagtaa tgaaaattta attaagctat
      421 gagtagaaaa atgtattaaa attacataat tttttagtta gattttaatt tagaaatggg
      481 attgtaatat tggaaataaa ataaggagga attttaattt gcaaaatgta aatttatttt
      541 tagtatttat agaaggtata gtatcattct tttcaccatg tatactgcca attttgccta
      601 tttatttgag tatattgtca aatagtagtg tagaaaattt aaaagaagga aagacgagtt
      661 ttataggaag ttctttattt aaaaatacta tattttttgc tcttggaatc tcaacaacat
      721 tctttatatt gggttcatca gtaaaagtat tgagtatgtt ttttaatgaa aataaagatt
      781 taataatgtt tattggtggg attataatta ttatcatggg attattttac atgggaataa
      841 ttaaatcttc aatattgaat agagaaaaaa gatttaatgt taagtttaaa gaaatgaaag
      901 caataacagc ttttatatta ggttttacgt ttagttttgg atggacacct tgtattggtc
      961 caatattggc atctgtatta gtcatggttt caagttcaag taatcatctt agtgccaatc
     1021 ttttaatagc agtatatact attggtttta ttttgccatt cataattaca gctatgtttt
     1081 acagtaaact atttaaaact atagataaga taaaatctaa catggaaatt attaaaaaaa
     1141 taggtgggat tatacttata gtatcaggaa tattaatgat ggtaaatgga tttggaagta
     1201 taagcaaaca ctttaatacg tcacaaaata gtaaaattga aagcaagcaa gaagaaaata
     1261 agcgagaaaa ttctactgat aaagaagaaa attctgatgg taatgatagt caaaaagact
     1321 ctaataatga caataatgat aaagggtcta atgatgaaga tagaataaag tctattgatt
     1381 ttactttaac agaccagtat ggtaaaacac ataaattaag tgattatgaa ggcaaagttg
     1441 tattcttaaa tttctgggct acatggtgtc ctccatgtaa agaagaaatg ccttatatag
     1501 aacaactgta taaagactat aataaaaata atgatgatgt agtaatttta ggagtagctt
     1561 cacctaattt aggtagagaa ggttcaaggg aacatgtagt taacttctta aaagaacaag
     1621 gttatacatt ccctgttgta cttgatgaag atggagcatt agcatatcaa tatggaatta
     1681 atgcttttcc aactacattt ataattgaca aggaaggata tgtaactcag tatattccag
     1741 gagctatgga taaggcaact atggcatcat ttattgaaaa tcagagaaat aaataaaagt
     1801 atttaagtat aagtttataa atcaatttaa ggttacaaaa aagtgcctaa ttgtaccacc
     1861 taaataatct tgttatagta aaagtataaa caatacaaga aagggttgaa ttttatatga
     1921 aaaaaacatt tgatgctata ataataggat ttggaaaagg tggaaaaaca ttagcagggg
     1981 atttggcaaa caggggatta aaagtagctt taatagaaaa gtcaaacaag atgtatggtg
     2041 gaacatgtgt aaatgtggca tgtataccaa caaaaagctt agaaaattca gcaaacagcg
     2101 taaaaacaaa gaatataaat tcttgggatg aagtacaagc tgaatatgag aaagctatag
     2161 ataaaaaaga aactttaatt acaaaactca gagaagctaa ctataataaa ttaaattcaa
     2221 atgagaatgt aactatattc acaggaatgg gaactttcat tgatgaaaaa acagttcaag
     2281 tcaaaactga aaatgaaatt tatgagttag tagcagataa tatatttatt aatactggtt
     2341 caagaccatt tatacctaat attaagggaa ttgaaaataa gaatatagta tatgatagtg
     2401 agtctcttat gaatctaaga gttttaccta aaaaaatgac aattatagga gctggattta
     2461 taggtttaga atttgctggt atttatagtt catttggagc agaagttaca atattaaact
     2521 ctaataatgg cattttacca aatgaagatg ttgaggattc agaagaaata ataaaattac
     2581 ttgcaaagag aaatgtaaaa attgttaata atgcaaatat aaaagagatt aaagaagttt
     2641 ctgaattagc aatagtagaa tatgaagtag atggtaaatc taaagaatta acaagtaata
     2701 tgatacttgt agctacagga agaaaagcaa acactgaagg gctagggcta gaaaatgcag
     2761 gtatagaatt aaatgaaaga ggatttatta aagtctcaga aactcttaag actaataaag
     2821 agcatatatg ggctatagga gatataaatg gaggtccaca atttacttat atttcattgg
     2881 atgattatcg catagttatt aatcagttat ttggagataa gactagaaca actaatgata
     2941 gaaagaatat cccaaattct atatttataa gtccagcatt ttcaagagtt ggtcttaatg
     3001 ttaagcaagc taaagaaaag ggatatgaag tattagtggc aaagatgcca gtagaagcaa
     3061 ttccaagagc taaacagatt ggcaaggcag atgggtttat aaaaatagtg atagataaaa
     3121 aatccaataa gatacttgga gctagtatga tatgtgaaaa ttcaagtgag attatccatc
     3181 ttattcaatt agctgtagat ttagaagtag agtatacata tttaagagac cgtgtttatg
     3241 cacatccaac tatgacagaa gctcttaatg atattctatc tcctaatatg ataaaagaag
     3301 tataacataa gtttaatata taaatctaaa attatcatta taag
//
LOCUS       DAEMGG010000221         3299 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEMGG010000221 DAEMGG010000000
VERSION     DAEMGG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.224"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            165..1988
                     /gene="typA"
                     /locus_tag="KPK24_003706"
     CDS             165..1988
                     /gene="typA"
                     /locus_tag="KPK24_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG2301332.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            complement(2078..2986)
                     /gene="trxB"
                     /locus_tag="KPK24_003707"
     CDS             complement(2078..2986)
                     /gene="trxB"
                     /locus_tag="KPK24_003707"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG2301333.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
BASE COUNT         1148 a          532 c          486 g         1133 t
ORIGIN      
        1 tatttgtcta aaaaattttt ttaaacagaa taattttcag atgcaaaact aacagtttta
       61 tccaatttaa actatattaa tacaatttaa tatattcctc cttgtaccta ataatatatt
      121 gtgctataat taataagtta ttttaatcga agggagcaac aaaaatgtca caaaaacata
      181 aaattataaa tatcgcagtt atagcccacg ttgacgctgg aaaatcaaca ttggttgatg
      241 ctttcctttc tcaaagcggt gtttttagaa agaacgaagt agttaaagac tgcgttatgg
      301 acagtaacga ccttgaaaaa gaaagaggca taactatata ttcaaaaaac tgtgcaataa
      361 actatgaaga ttacaaaata aacatagttg atactccagg acacagtgac ttttcttctg
      421 aagttgaacg tgttatgaaa actgttgaca cagttattct attagttgat gctagtgaag
      481 gcccaatgcc tcaaactaga ttcgttttac aaaaatcttt agagttcggt ttaaaaccaa
      541 tattatttat aaacaagatt gataaaaaag accagagagc ggaagaagta gttaatgaag
      601 ttttcgattt atttgtagac ttaaatgcaa cagatgagca atgcgaattt cctataatat
      661 atggtattgc taaacaaggt attgctaaac ttgaaatgga tgatgatagt gaagaccttt
      721 ctcctctatt taaaactatt gtaaatcacg ttgaagctta tcctaattac gataatgaac
      781 ctctacagtt ccaaatatca gctcttgcat atgatgatta tgtaggtaga cttggtatag
      841 gtagaattta caaaggtact cttaaaaaca atacacaagt tgctatatgt agagaagatt
      901 ctgttgtatc taaaggaaaa gtatcaaaac tttctgtgta tgaaggttta aaacaagttg
      961 aagttgatga agctactagt ggagaaatag ttgttatagc tggtattcct gatatatcta
     1021 taggtgaaac tatatgcgat ttagatagtc cactacctat ggaaatgata aaaatagaag
     1081 agcctactct ttctatgaac ttcttagtaa acgactctcc attcgttgga aaaagtggaa
     1141 aattcgttac aactagacat ttgaaagata gacttgaaaa ggaacttgaa gtaaatgttg
     1201 gtcttaaagt tgaacctctt gatactactg atggatacaa agtttctgga cgtggagagc
     1261 ttcacttatc tatactactt gaaaatatga gacgtgaagg ttatgaagtt ggtgtttcta
     1321 aacctgaagt tttaatgcat aaagaagatg gaaaactaat ggaaccaata gaaagagttg
     1381 ttgtaaactg tcctgaagtt tactctggta ctattataaa tgaattaaac atgagaaaag
     1441 gtatgatgga gtctatgtca atagaaggag actatgtaaa aatagagttc ttagcaccta
     1501 ctcgtggtct tttaggatat cgaagtgaat ttataaacgc tactcgtgga gaaggtactt
     1561 tagttcgttc atttgaaaaa tttgaagaat ttaaaggaga aataccttca agaggaaatg
     1621 gtgttttaat agcacaagga cctggtgtta ctatggggta ttctcttaat gccttaagtg
     1681 atagagctgt aatgtttgta gaccctggtg tagaagttta cgaaggtatg ataattggta
     1741 tgaattccag aaaagatgat atggtagtca acccttgtaa aaacaagaaa atgagtaacg
     1801 tacgtgcttc tggttcagat gatgctataa aattatctcc tcctagaata tttactcttg
     1861 aagaagcact agagtttata gaagatgatg aattagttga aattacacct gattctataa
     1921 gacttagaaa aagattctta aatgaacatg atagattaag atataacaaa tctagacaag
     1981 gtaaataaaa cgtacattta cctctaatta acaaaatagc catatagtac tgaaaaatta
     2041 tttcagttta tatggctatt tttatttgaa atttatatta ttcaacattt atataattag
     2101 ctgcttgaac ggctgcaata gctccatctg atgctgctgt tattatttga cgtaaaaact
     2161 tagttctact atctcctgct acatatacac cttctactga tgttgtacaa gactcatctg
     2221 aaattatata tccaccttca tccaaatcaa gaacattttc aaacatttta ttattaggtt
     2281 taagacctat agcaataaaa ataccactaa catctatagt tcttttttct tcagtcttta
     2341 agttttttac ttctattttc gatacagtct tttctccttc tattttttct acaccatgac
     2401 tgtaaagtat ctcaatattc tctcttgctt tcacagattt ctccaacact tcttcacctc
     2461 taaaactatc tcttctgtga attaaataca cttttgtaca attattagct aaaaataaag
     2521 catcttctag tgcagtattt cctccaccta ctatagcaac ttctttatct ttaaaaaagg
     2581 ctccatcaca tgttgcacag taagatactc ctctacctgt aaattcttgt tcccctgcac
     2641 aacctaattt acgtctttca actccatttg caagaataac agcctttgct ttatgttcac
     2701 ctgaagaagt ttttacaacc tttacttttc cctctaaatt tatctcttca acctcatcaa
     2761 actgaatatc aacaccttga gcaactgctt gattgtatat attattagaa aattctaccc
     2821 cagaaatctt ttgaactgct ggataatttt ctacctcaga ggtacttgca acttgacctc
     2881 cataaatatt tttttcaaat actgtaacgc ttaaccctgc tctcatagca taaattgctg
     2941 aagttaaacc tgctggtcca gcaccaatta caatgatatc taccataacg tttacctctc
     3001 tttatattat tatttcttat atattattct catcattctt atactttact attattatcc
     3061 atatatacta taaacaaaac attatttttt attaattaac tttattttac atatatataa
     3121 aaaaactgca aataaaaatt aacttttatt tacagttctc acttatttat ataattcaaa
     3181 accttattgg ttatcattta cagtgtcttc taatatattg ctttctgata tatatgatag
     3241 aattgaaagt ccatacataa ccaaaactaa tgctgctgtt ataagtacta ttcccatat
//
LOCUS       DAEMGG010000222         3207 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEMGG010000222 DAEMGG010000000
VERSION     DAEMGG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.225"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   23..909
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=23..51
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(1105..1344)
                     /locus_tag="KPK24_003708"
     CDS             complement(1105..1344)
                     /locus_tag="KPK24_003708"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301334.1"
                     /translation="MECYLKRTLIIFSEAFLSISFKTRKYLFIVIFILVCPNLSEMIF
                     ILVPARRRIIRIDNIVSIYKYVGVLKLINYSILIL"
     gene            1556..2827
                     /locus_tag="KPK24_003709"
     CDS             1556..2827
                     /locus_tag="KPK24_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2301335.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1267 a          422 c          404 g         1114 t
ORIGIN      
        1 gataggtcac aacttaagag tgatttacat accacatagt taatataaaa ctctcttgtt
       61 tcttctgtta attttattat accttcatta tttacatacc acatagttaa tataaaacgc
      121 gttatattgt tgtataaaac cgattaaagt tcaatttaca taccacatag ttaatataaa
      181 acttgcaaca tattgtttta tcgttgctgg gtctttaaaa ccatttacat accacatagt
      241 taatataaaa ctaagcgata ttagagagcg caaggcatag cggagcaaat ttacatacca
      301 catagttaat ataaaacttt aattttaacc accccctttc actaataaaa aaagatttac
      361 ataccacata gttaatataa aacaagacgc tatacaagag gtaatttgaa agcaatctaa
      421 tttacatacc acatagttaa tataaaacgt tgcatatgta tgtctaagag catgaaattt
      481 aattttattt acataccaca tagttaatat aaaacttcta ttaagtgtaa gcaaaacgct
      541 tggaatatga aatttacata ccacatagtt aatataaaac ttatacacaa atacaacttg
      601 aagaagcgct aaagagattt acataccaca tagttaatat aaaacctaat gagtacatag
      661 ataaacgtac aggagaggtt aatttacata ccacatagtt aatataaaac gttgataatt
      721 ttcgtttgta tactttttat aatttaaaat ttacatacca catagttaat ataaaacata
      781 aagatttaga aaaagaaaaa gttaaaattt tggaatttac ataccacata gttaatataa
      841 aacttttcct gttaagttga tgtttgcaac tttaactgct atttacatac cacatagtta
      901 atataaaacc ccaaaataaa ctaagtattt tcagtactta cacacataca actctctcaa
      961 atttgcagtg aaccatgagt agtgcaattg ataacattta tcacacaccc tcaatccctt
     1021 acattgcaac tgttaaaccc tactttatca aaaatatcgc tcactgcaaa atttatatat
     1081 ttttattata tcataaaatt caaactataa aattaaaatg gaataattta tcaatttaag
     1141 aacacctaca tatttgtaaa tacttactat attatctatt ctaataattc ttcttctagc
     1201 tggaactaat ataaaaatca tctctgaaag attaggacat actaatataa aaataactat
     1261 gaatagatat tttcttgttt taaacgatat ggataaaaaa gcttctgaaa atataatcaa
     1321 agttctcttt aaataacatt ccattttatg taagtaggca ttatattttt cttaaatgtc
     1381 acttaaatgt cagttatata taataatagt aaacaactgt atttcactaa tatttgctat
     1441 tatctaatag tatttatagt ttagatttga tataatacat gtggtaaaat tttgatttgt
     1501 aaaacaaaat tttacctaca tatattaaaa caaatttaaa actaaggaga cctatatgtt
     1561 aaaagtatta aaaggaaata taatttttac aaaaacttca gaaactttca cagtatttga
     1621 aaatagttat attatactgg aaaaaggtaa agtaaaggga atatttaaag agatacctaa
     1681 ggaatacaaa aacctaaaag tagtagatta cactgataag cttataatac ctggtatgaa
     1741 tgacctccat gcccatgcct ctcaatttaa aaatcttgga atggctatgg ataaagagct
     1801 tttgccatgg cttgaaactt atacatttcc agaagaatca aattataaag atatagaata
     1861 tgcaacaaaa atgtataaaa aatttgtaaa agaactttgg aaatctggaa ctacaagaat
     1921 agcagtgttt gcaacagtac ataaagaagc ctctatgaaa cttatggata tattcaaaga
     1981 agttggtctt ggagcatacg ttggtaaagt aaatatgaat atgaattgcc ctgatacttt
     2041 attagaaaac actcaggatt ctattactga tacagaagaa attataaata agtatagtga
     2101 tacagattct attgtaaaac caataataac acctagattt ataccaagct gtacaagtga
     2161 acttttaaaa ggtttaggag atttatgttt aaaatataat attcctattc aatcacactt
     2221 atctgaaaat tatgatgaga ttgaatgggt tagaagtcta gaacctgaat ctaaattcta
     2281 tggagatgca tataatagat ataatttatt tggtcaaact cctactttaa tggctcattg
     2341 tgttcattgt tctcaggatg aaatagattt aatgagagaa aataatgtta tggcagttca
     2401 ctgtccagct tctaacttta atgttggtag tggtgctatg cctatcagaa aattaatcga
     2461 taataatatt agattagcac ttggatcaga tataagcggt ggacatacat tgtcaatatt
     2521 taaagcaatg gtatctgcaa tacaattatc taaattatat tgggttaatt ctggtaagaa
     2581 gtataatttc ttatctttat ctgaggcttt ttatattgca accaaaagtg gtggaagttt
     2641 ctttggaaaa gttggtagct ttgaagaagg ttatgatttt gatgcattga ttattgatga
     2701 ttcaaattta aatcatgaca attattctat acttgaaaga cttgaaagat ttatctatgt
     2761 tggtgatgat agaaatatca ttcatagata tgtatgtgga aaactaattg aagaacctaa
     2821 tatataatta taataaaaat tttaattata tattatggtt aatttataca actataatta
     2881 ataaaagagt atattttaaa tctaaaagct ataggttgaa acatactctt tttattttgt
     2941 ttatttatag ctttagttat taatcatata taaatctaca aaacgtattc ctagtaactt
     3001 atattttttg atatctacaa tcataagact atattattgg attcatacct caaaaatatt
     3061 gccttctgaa cattattata ttcaaagcta tgttaattta caatgatata tcctaacatg
     3121 tattgattaa aaaaaattaa aacttgcttt tcatctccta tatctaaaga agtggtattt
     3181 agaataaact attgtaatat taaagtt
//
LOCUS       DAEMGG010000223         3157 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEMGG010000223 DAEMGG010000000
VERSION     DAEMGG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.226"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..744)
                     /locus_tag="KPK24_003710"
     CDS             complement(<1..744)
                     /locus_tag="KPK24_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG2301336.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKK"
     gene            complement(784..1131)
                     /locus_tag="KPK24_003711"
     CDS             complement(784..1131)
                     /locus_tag="KPK24_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301337.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1349..2305)
                     /locus_tag="KPK24_003712"
     CDS             complement(1349..2305)
                     /locus_tag="KPK24_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG2301338.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2748..3116
                     /locus_tag="KPK24_003713"
     CDS             2748..3116
                     /locus_tag="KPK24_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG2301339.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         1137 a          424 c          403 g         1193 t
ORIGIN      
        1 ttttttactt atatttagta tagttaatat ttgtttaaga tatattatta tatcttctgg
       61 agttctatca aatttgagtt caaaattttc atcttcaaag tagggaatat aatataaagg
      121 tgttttgaga gcaggtaatt tattatataa ttcttcaaga atatcgatat ttttagggga
      181 gttaaagaaa aagcatttac atacattcat taaatccaca gcatctgaaa gtgagttatg
      241 tttaatatct ccttctacag aaaaaatttt tttcatattc agtaaagata tttgcttaga
      301 taagattcta ttattgtatg taatatgttg agatataaaa ggttgaatgt caatcatcat
      361 attaaataac tctaagcatt tacctgtata tttatacact ctaaatgaag ttagaaggcc
      421 agttatatca aaatcacccc aattatatat tgtaacatca tcaaatttac aaaaccattt
      481 ataaaaatca tccataacat ataaaagatt aggagcatta ttgatatcta gttggttcaa
      541 tttggttaaa tttttacatt tatttgaaag tattttattg tattttggct taactaaaga
      601 gtaataatta tctattgtat taaattcgtt atcaactaaa actccaccaa tagaaattaa
      661 ttccgcacca ttatattctt ttggtttatt tttattatca tctagtatat taaattcaaa
      721 gtctataaat aacttgtatt tcataaatct aaagcacatc ctcaggtaaa ttatttattg
      781 agcctaagca tcataagaat caatagatat aatgactcta aacatcaaag gttcattaca
      841 acttttattt atttcataat ttaattcaaa taaatctttt aagatataat ttataagtat
      901 tctttcccaa cccattttta aattaacttt agagtctatt aatatatttt ctatcttttt
      961 aaatatttct atatttgctg gtattatact gagctcaata ttacttttaa acgggttaga
     1021 ctgtaaatct gttataagta tattttttaa ttcctctgta agttcagaaa ttaaactatc
     1081 acctatgtat ttttttatga tttcctctct aatttctttt aaatttagca atattagtac
     1141 ctgctactta catagtagct tttcctttcc ttttaatact attattcatt tttgaatctt
     1201 atttagatat attttaagat taaaagtctt tagctatata attttttacc catttttaac
     1261 ttatattaac atattacagg cttatatctc caccctgttt acattgagga tggagtatat
     1321 tgcctatttt gataaaaatg ataggttgct atttgcctat tcctttttct gataattttt
     1381 ctaacatatc tttggtcatt ttatctaaat cataactaaa cttaaagttc cattctttta
     1441 ctgcactact actgtctaga gagttaggcc aagaatcagc tatactttgt cttacagggt
     1501 ctacatcata ttctatcaca aaatcaggaa tatatttttt tatagaatca gctatttctt
     1561 caggagaaaa gctcatagca gtaatattaa aagcatttct atggatgagt ttatcagctg
     1621 gagcttcgat taaatccact attgattgaa gtgcatcagg catatacatc atatccattt
     1681 ttgtaccttc tgctatgtaa gatttatatc tcttattttt taatgcctca taatatatat
     1741 caactgcata atctgtagtt cctccaccag gaggcgtaac ataagaaata agacctggaa
     1801 atctaacacc tctagtgtct actccaaatt ttgagtgata ataatcacat agtaattctc
     1861 cagaaacttt agtcacacca tacatagtat taggtctttg taatgtatct tgaggagtca
     1921 tattttttgg agaattttca ccaaaagcag caattgaact aggtgtaaaa aatttacaat
     1981 ccaattcttt ggctatttca agaccattta ttaatccatt catatttaag ttccaagctt
     2041 ctagaggttt actttcagca actgcagaaa gtaaagatgc tagatgaatt atagtatcta
     2101 catcatattt ttttgcaatt tcaaaaaaac gattcttatc tgtaacatct aatatctcaa
     2161 atataccaga ttcacatact ggattacctt cttttactct ccttgaacta gcgattacat
     2221 tttgttctcc atactcattt ctaagcttta ttgttaattc tgaacctatt tgtcctaatg
     2281 cacctgttat aagtattttt ttcaatttta caacctccta agggtttaat ttatgtagta
     2341 ctacttattt ttattttagc aaatcttttg tttaaataca aagaattaaa gataataata
     2401 tacgacttaa ttgaatgaag tgcagtaagt gcgtaaaaat ttaaagctaa tatataatgg
     2461 tctattgtat aacaataaaa gagtagatta aattattata tatagattgt gtgactaagt
     2521 cacagaaaat agagctaatt taatatataa taaaagcatg tcaagtaaag ctttaaatta
     2581 aagtaacttt atataaaaag taatataaat tgataaaatt ataaatttat ttatagtgaa
     2641 tataggttgg attttataga tacaaaaata gtatatatga tatgtagacc attagcatgt
     2701 ttaaagtagc aaaacatata aaataaaatt tgaaaggaag tataaaaatg acaagaccat
     2761 caatatatca tagccaagga tacacttgtg cagaggcatt aataaagtct tataatgagg
     2821 aacacaacac agatatacca atctcaatag gaagtggtat gggggttgga atgaatgttg
     2881 gaagtgtttg tggagctgta aatgctgctg ttgttataat agggtattta aatgggcgaa
     2941 atagtaattt agatgaaaat atagcaagga agtattcaag agagctattg aatagagtaa
     3001 gaaataaata cagctcagaa atatgtgtat ctcttaaaaa aaataaagta acttgtgaag
     3061 aaataataaa tttcacatat gatgctctaa atgatatgct aaaaaatcaa aaataattct
     3121 aagaaacaag tattttacat tcgatagaat acttgtt
//
LOCUS       DAEMGG010000224         3122 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEMGG010000224 DAEMGG010000000
VERSION     DAEMGG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.227"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            282..935
                     /locus_tag="KPK24_003714"
     CDS             282..935
                     /locus_tag="KPK24_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG2301340.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVNASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            1011..1220
                     /locus_tag="KPK24_003715"
     CDS             1011..1220
                     /locus_tag="KPK24_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301341.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(1360..1926)
                     /locus_tag="KPK24_003716"
     CDS             complement(1360..1926)
                     /locus_tag="KPK24_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG2301342.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            2087..2740
                     /locus_tag="KPK24_003717"
     CDS             2087..2740
                     /locus_tag="KPK24_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG2301343.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(2898..3074)
                     /locus_tag="KPK24_003718"
     CDS             complement(2898..3074)
                     /locus_tag="KPK24_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301344.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         1183 a          326 c          449 g         1164 t
ORIGIN      
        1 tgtagaaaaa atataataaa agtgtgaggc ttagtaatta acaaattatg tggcttcaca
       61 cttttttata aaataaagat acttagcaga ataatatttt agttgatatg tttataagat
      121 ggtacttgtt tgggtaaaag tataaatagt aaaaaagtag aagtaatgtg tataaaaaca
      181 taagttacat tctgtaataa tagttatttt ataattcaac aaaagaatag aatgggtata
      241 tttgtgaaat aataatataa aataatctta ggagtgagct tatgaagaaa aaattattat
      301 atataaatgt aaattcaaaa cctgaagact tatcttcaag taaaactgta gcaagaaaat
      361 ttataaataa atttatggaa aaaaataaag attttgaggt tgaagagata gacctttata
      421 aagaacatat accaagactt gagtatcagt actttgaaaa aagaaatagc atagttagtg
      481 aagaaaatgc taaaaattta gacgataagg atagaaaaga gcttacaaag attagaaatc
      541 tttgtgacca atttgtgaat gcaagtgttt atgtaatagc agcacctatg tggagtttat
      601 cttttccagc accacttaaa gagtatatag attgtataat tcaagatgga aaaactatat
      661 cttttgaagg caatgataaa ccacaaggaa ttttaaatga tattgataga agtatggtat
      721 atatacaatc ttctggtgga cacataccat gggtgttgaa gccagttatg aataaagggt
      781 taaattatgt ggaaagtatt atgaaattta taggaattaa aaaatttgat gaattgttag
      841 tagatggaac tggaactagt gaagaagaaa gacaagctgc aatagaaaaa gcatctgaaa
      901 aaatagatgg tataatagat gggatgaaat tttaattata atattttatt aaaaatataa
      961 aggaaaaacc tcttttaatg tagaaactta tattgaagga ggcgagaatc atgcgagaag
     1021 aaaaaagtaa tgaaaagtat gattgttatt ggtgtaatca agagaataat ttctgtgtag
     1081 aaataaaaga taatatagtc atgatagatg atggcactgg tacgttaaaa caagccgttt
     1141 tcatagggta taaacaaatc caaattaatt taaactgttc acattgtcaa aacttaaata
     1201 gaataaaatt aaatttgtag aattttaaaa tggaaattat tgaaggaatt aaaaatagaa
     1261 agtgttaata tttagactga aaattatatt ttatacaata aaagtttatt taaataacat
     1321 tgtattttaa tatttatttt cagtcttact ttattaaaat tattttttgg ttatgtctgg
     1381 aaaatatctg tctactaatt tttctgaaat attttttaat tggtcagcca ttgaagtcag
     1441 acttaataag gctatcaata aatcatcttt attttctgga tttatactaa tattttcaaa
     1501 attagactct ataaagtccg gtatattctt tctttgattt tcttttatta acatgaattt
     1561 ttcgtaacat gaaactaaat ctttttcact gaaattatcc ttttcataca caccatgtaa
     1621 gattctttta atttcttgta ttgtaagtgt atttttcata gaataaataa ttatcatctg
     1681 taaaatttgt tctttagtat acttttttcc cttaactggt ttgattaatc cctctttgct
     1741 gtaattattt atcatggttt tagtaagaat tttatcactt tcaaatcttt tattagcaga
     1801 aagcttattg tcaattagag aaataatttg atccatatat aagtcaatag atggaatatc
     1861 gtttgaagtt atatctgcat tatttagtgt ttctagtatt atagtattaa gttcattatt
     1921 catcatatgt tacctccata atatatataa tacactaaat tagaaagtaa aacaatatta
     1981 aggcttgtaa aaaaaacata aagtggtata attaaatata taatatagta atcaaaacta
     2041 tatttatgaa tctgattttt aaaatacata attgtaggag gacgaaatgt atcaattttt
     2101 tttaaagggt agagacccaa taagcagttt aactcatttt ataggtgctt gtttatctct
     2161 tttagcaact ataattttag tttttcaatc tgttacatta caggaaactt ctctcttgat
     2221 gatagtatct gtatctgtat ttggactttc actaattgct ttatatagtg caagttcgta
     2281 ttatcatttt ttaaagggga caccagaaca agaattgttt tttagaaaag tagaccatgc
     2341 aatgatttac gtattgattg ctggctctta tacaccaata tgtctgaatt ttatggaaaa
     2401 aaaagaaggc ataatatttg ttacagcaat atggatagtt gcatttattg gcataataat
     2461 aaaaatattt tggatggatg cacctagatg gttatctacc agtatatatt tacttatggg
     2521 gtgggcaatt gtttttgata taaatgcatt caattcaatt cctaaggatt gccttagact
     2581 attaattatg gaaggtgtat cttactctat aggtgcgatt atttatataa ttaaaaagcc
     2641 taatataagt cctgagtttg gatttcatga aatatttcat atttttatta tgataggttc
     2701 tttatttcac tttttagcag ttttattgta tgttttatag ttttaaaaga tttgattaat
     2761 tgatactaaa attctacaat gaataaaatg gaactgcctt atatagatta tatcttagac
     2821 aataagacag ttccttatta taaatattaa attctactta taataaaatt ttgataaaaa
     2881 tagtccattc aaaatgccta ttccataatt cctttaatgt acttttcatt tacgacatat
     2941 agtttgaatt tatgtactat aattatatcc tctaaattct ctgaaacact aacaattctg
     3001 acacatttgt ttctaatttc tacaggtaaa tctaaatcat ttgttaaatt cacatagtca
     3061 tcatatttat acataaaatc atccctttac attaagatat acttattata ctatatttta
     3121 at
//
LOCUS       DAEMGG010000225         3117 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEMGG010000225 DAEMGG010000000
VERSION     DAEMGG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.228"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            58..2415
                     /locus_tag="KPK24_003719"
     CDS             58..2415
                     /locus_tag="KPK24_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG2301345.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2501..2818)
                     /locus_tag="KPK24_003720"
     CDS             complement(2501..2818)
                     /locus_tag="KPK24_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG2301346.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1203 a          336 c          503 g         1075 t
ORIGIN      
        1 ttataatata tttgtagatt tgagtataaa aaagtgctaa gttgaaagga gtaaaatatg
       61 gataaggtta atttagaaag gtatagttgt gatataaatg ttggtttaaa taaaaatcaa
      121 cttcaaagta gaatagatga taatttaatt aatgcatttg ataatggaaa aaccaaaaca
      181 tataaacaaa tttttaaaga caatatattt acacttttta atctcataaa tattgtttta
      241 gtctgtttat tagtttcagt tggttctttt aaaaatacct tatttatatt tattgctgta
      301 attaatacta ttataggagt aatacaagag ataagagcta aaagattatt ggacaatcta
      361 tctgttatag tttcaaataa ggtatctgtc ataagagaaa aagagaaaaa agagattgat
      421 gtacaagaat tagtattaga tgatattatg attttaaaaa caggtaatca aatttgtgca
      481 gattcaaaag ttttaaatgg aaaattagag gtaaatgagt ctttggttac aggtgaatct
      541 gatattatta taaaaaataa aggcgatttt ttatattctg gaagttttgt agtttctgga
      601 gaagcttttg taagagttga aaatatagga aaagataact atgcaaataa gatagctaat
      661 gatgcaaaga tttctaaaaa acataattct caacttagaa attcattaaa tgcaatatta
      721 aaaattgtag gtataataat aattccttta ggaattatat tgtttgcaaa acaacatttt
      781 ggaaatggag attctatagc tacatctgta gtaggtacag tggcagctgt aaatggtatg
      841 ataccagagg gattaacatt acttacgagc attgctctag ctgtaggaac tataaaactt
      901 gctagtcata aaactttagt gcaagaactg tactgtgtag agactcttgc tagagttgat
      961 acactatgtc ttgataagac aggtacaatt acagaaggta aaatgcaagt tgatgatata
     1021 gtaatgttgg aagaagtgga tattaatgaa ataatgggga atatatgtaa ttctctcaaa
     1081 gatgataatg caacactaaa tgcaataaga gataagtata atgttttaga tacgtataaa
     1141 gctaagaatt taataccatt ttcttcagaa agaaaataca gtggggttac ctttgaaaat
     1201 aaaggtacat atttattagg tgcatttgag tttatattta aggaaaaaaa taaaaaactt
     1261 aggatagaag ttgaaagata ttctaaaaaa ggaaacagag taatagtttt agctcatagt
     1321 gattcttata tggaagataa taatattcct gaagatataa agccactagc ttttatattg
     1381 atattggata aaattagaga tgaagctaag gaaactttag aattttttta caatgaaggt
     1441 gtagatataa aaattatatc tggtgacaat cctataacag taagtgaggt agctagaaaa
     1501 gcaggtctta aaacagctag taattttatt gatgcaacaa cacttaaaaa tgatagtgat
     1561 atatacaaag cagttgataa atacagtgtt tttggaaggg taagcccaca acagaaaaaa
     1621 caaatgattt tagcattaaa aaaacaaaat catacagtag ctatgactgg agatggtgta
     1681 aatgatgtac tagcacttaa agaagcggat tgtagcattg ccatggcatc tggaagtgat
     1741 gttactaaga atgtgtctaa tttagtctta ttagattcaa actttgcatc aatgccaaag
     1801 gttgttatgg agggaagaaa agtaattaat aacattcaaa gagcatcttc tttgttttta
     1861 gtaaagacaa ttttttcatt cttcttgtcg ttattaacat tattcttctt cagtagacag
     1921 tatccatttg ttcctataca attatcactc attggggctg taacagtggg tataccatca
     1981 ttcttcttag ctcttgaagc aaacaaaagt agaatttcag gaaatttttt actcaatata
     2041 ttaaaaaatg ctttaccagg agcactatgt gtgacagtaa atgtaattat tgtttacaat
     2101 atagttgaat atttaggtta tagttcaagt gtattttcca caatgtgtgc tatattaact
     2161 ggtgtatgtg gtcttttaat attgagacaa caatgtttgc catttaataa agaaagatta
     2221 tttttaatag taagtatgac tattgcattt gttattggga ttctattttt aggtggatta
     2281 ttctcatttg tagcattgag tagagaatta attctcataa ctatggcatt agcaatatta
     2341 atgccaatta tgataaaagt tatgttattt gtcttagatg agatattaga gacaattgtt
     2401 ccagatttaa attaatttta aaatgatata aataagagca gttttagttg aactgctctt
     2461 tttagatgtg tttaatttta tgtaagaata tgtattttta ttaatcagct atatatgagc
     2521 aacagtagtc aacaacttca ttaaccttta agtcaaaact tgaatcacta ggaacattaa
     2581 atttttgccc ttctttataa gttacaaact cattgcttcc aggcagtttt acatccatac
     2641 ttccagcaag catctccatt atttcttctt ctctagtaga aaaagtgtat tctcctggca
     2701 acattaatcc taaagttttt ctttctccat taggcagtat tattactctg ctacttacct
     2761 taccatcaaa ataaacgttt gccttcttta ctgcagtaac attcttaaat tcactcatta
     2821 gataaaacct ccaaaattat ttgtacatat atattatata aaaataatgt aaaaaatgct
     2881 atatatttac aaaaaaattt agtatttatt aaaaaaatta ataagaataa aaaagaggtg
     2941 aaattgctta aattgataaa aaatataaaa taaattgcaa agtaagcaag aaaagaataa
     3001 gttgaagagt tatatattat aaatattaaa aattttagta ttaataaaaa agatattaca
     3061 ttaatatgaa aatataggag attagaagtg tttgattaaa aaatagctac acacact
//
LOCUS       DAEMGG010000226         3071 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEMGG010000226 DAEMGG010000000
VERSION     DAEMGG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.229"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            170..964
                     /locus_tag="KPK24_003721"
     CDS             170..964
                     /locus_tag="KPK24_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2301347.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            965..3025
                     /locus_tag="KPK24_003722"
     CDS             965..3025
                     /locus_tag="KPK24_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2301348.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1182 a          317 c          504 g         1068 t
ORIGIN      
        1 aaaatgttac aaaagatatt ataaacgaat aaaaaataat aatttgtcag ataaacttgt
       61 aaatttttat gaataatcca ttctaaaaca aaaaactgtg tataaaaaag gtttatatgt
      121 tatatttata taaatataac atatgaacga gaaaggagtg gtttgataaa tgattgaggt
      181 agataaattg tgtaagtctt ttacaagggt tgtaaaagat gataagaata aaagttcaat
      241 aagaaagcta aagaaagtaa aaactaaaaa agaagaattt tttgctgtaa ataatgtttc
      301 atttaaagtt atggaaggtg aaattgttgg aatattaggt ccaaatggtg caggaaagac
      361 tacattactt agaatgttag gtggcatatt aacaccaaca tcaggaagta taaatatttc
      421 tggatatgat tattcaatag atagaaattc agcaaaaaaa gaaataggtt atctctctgg
      481 aaatacaaaa ctatatggaa ggctttctcc gagagaactt ctaacaactt ttgcaagtct
      541 atatgagatg tcaaaagaag atatagaaga atcaattgaa aatattgtaa aaataatgga
      601 tatgagtgaa tttatagata atagaattga aaatctatca actggtcaga ctcaaagaac
      661 atctatagct cgttgtttaa tacattctcc aaaagtatat atttttgatg agccaacact
      721 aggtctggat gttttaagta gtgcatctat tatagatttt atgaagaatg aaaaactaag
      781 gggaaaaact gttctttact caactcatta tatggaagaa gcagaaactt tatgtgacaa
      841 gatttttatg attcacaatg ggaaaataat tgcatcagga acaccacaga gtttaaaaga
      901 ggaatctgga gtaaataatt tgagagatgt attcataaaa cttgccagaa aggaggacat
      961 ttaaatgaga agtaagattg taaaatacat atttaaaaaa gaaatgttag acatattgag
     1021 agataaaaag actttattta tgatgattgt tgtgccactt cttttatatc ctttacttat
     1081 gttattaatg attcaagtaa tgaatatgtc tgcaagtagt atgaatagta aggacattaa
     1141 tatagctttt aataataaac ctaataaggt gttagtatcc atgattgaag atgataattt
     1201 agaaaaagat actaaaaata cttcaaatga taaaggcaaa ataagagtgt tagatgtaga
     1261 taactataaa aagtcattag aagaaaataa aattgatgct tatataaaga tggaagaaaa
     1321 aaaatcatct ataaattatc aaatatatat aaattcttct caagaaaatt cttctaatgc
     1381 actaaaaaga attgaaactg ttttggataa atataaaaga tttaccatag aaaaaacttt
     1441 atctgaaaaa ggattagatg tacaagctac tttagaacca ataacatata gcactgtaga
     1501 tgttgcaaag acggaagaag tggcaggata ctttttaggt cagatacttc catttatatt
     1561 aataatagga gttttactag gttcaatata tccagcaata gatgttatgg ctggagaaaa
     1621 ggaaagaggt acattagaaa cattatttac actgccaata tcaaacttag aactggttat
     1681 gggaaaatat atggcagttt ctcttagtgc aatagtaaca gcaattttaa acattttatc
     1741 tatgggactg actatggcat ttattttagt tagtggaggt attagtagcc aatttggctt
     1801 tggaaacttt aattatggag acttagtctt accaattata actactttaa tttgcatatg
     1861 tcttttttca atggtagttt cagcaataag tatgtgtgta tgttcattag caaaaagttt
     1921 taaagatgct caaaactaca taacacctct tatgttaatt attatgatac catcatatgt
     1981 ttctatgata ccaaatatag tattagatag agtgacagct gtaatacctg tagtaaatat
     2041 atctttactt ataaaaagtg tattatcttt taaaagcgat ttaaatttaa tagccttagt
     2101 tttagcatca aatgtagcct ttgttatatt ggcaatcata ctactgtcta agatgttcaa
     2161 ttctgaggaa atactttttg gtaatagtaa gagtttttct ttcttagaga aaagaagtaa
     2221 tataaaaaaa gggtctatac caactgtaag tgatggtgtt attttatatg cagtaggatt
     2281 agttctattg atttatgttg gttctttagt tcaaattaaa tttggtatgg caggtattat
     2341 aatgacacaa ttcatgataa tttctttacc attattattt gcatactata ttaaagctga
     2401 ttttaaagag gtatttagct tgacagttcc taaattaaag catgtgtttg ggagcattgt
     2461 tctttggatt ggtggataca tacttattat gattataaca cagctaattt tattcttatt
     2521 tcctcaaaat atggaagttg ctgaaagttt aaataaggct ctttttatca aagatagtgt
     2581 tttactgaat ttactagcaa tagctttatt accagctata tgtgaagaaa tgttttttag
     2641 gggatttata ttttcttcat ttagtaaatc aaaagataaa aataagtcta tcaaactcgc
     2701 tataatatgt agtggggtac tatttggtat tatgcatatg gattttatta gaataattcc
     2761 aacatcaata ttaggtatta tatttgcata cagtgtgtat aaatctggtt caatatttgt
     2821 atcaatgtta ttgcattttt taaataatag tgtagcagtt gtgttaaatc attatacaac
     2881 tggaaatata accaacttat ataaatttgt tgaagttgat ttttcgaact taaatgtgct
     2941 taaatttgcc ttaattcttc taataagtct tatattaatt atgttaggat taagagtttt
     3001 agatagaaag tctttaagaa attaagtata aagttattat atagattaaa atattaataa
     3061 gcactaaagt a
//
LOCUS       DAEMGG010000227         3062 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEMGG010000227 DAEMGG010000000
VERSION     DAEMGG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.230"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1228..1617
                     /locus_tag="KPK24_003723"
     CDS             1228..1617
                     /locus_tag="KPK24_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2301349.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            2351..2821
                     /locus_tag="KPK24_003724"
     CDS             2351..2821
                     /locus_tag="KPK24_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG2301350.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
BASE COUNT         1400 a          173 c          539 g          950 t
ORIGIN      
        1 atttataata taattaatga aaaaatttaa actttgtaat atataaataa aaaaactcta
       61 aaatagaagg atataattat atagaatgtt tcaattaaac aaggcaaaaa ctataatctt
      121 aaaattaaca ttattcttaa gaagtaagaa atttaagaag taaaaagttt agttataagt
      181 ggtagtaagc aagaaattta agaagtaaaa agtttagtta taagtggtag taagcaagaa
      241 atttaggaag tgggaagttt agttataagt ggtagtaagt aagaaattta ggaagtggga
      301 agtttagtta taagtagtag taagtaagaa atttaggaag tgggaagttt agttataagt
      361 agtagtaagt aagaaattta ggaagtagga agtttagtaa taatttgtag caagtaagaa
      421 acttaggaag tgggaagttt agtagtaata atttatagca agtaagaaat ttaggaagta
      481 ggaagtttag taataatttg tagcaagtaa gaaatttaga aagtaggaag tttagtaata
      541 atttatagca agtaagaaat ttagggagta ggaagtttag taataattta tagcaagtaa
      601 gaaatttagg gagtaggaag tttagtaata atttgtatca agtaagaaat ttagggagta
      661 ggaagtttag taataattta tagcaagtaa gaaatttaga aagtaggaag tttagtaata
      721 atttatagca agtaagaaat ttagaaagta ggaagtttag tagtaattta tagtaagtaa
      781 gaaatttagt aagtgagaag tttagttata atttatagta agtaagaaat ttagtaagtg
      841 agaagttcag taataagttg tagtaaataa aaagtttagg aagtaggaag ttcagtaata
      901 agttgtagta aataaaaaat ttaggaagta ggatttactg aaatacaaat atttaaaatc
      961 aaagagttta aaagaaagga gagctactgg acaagtatcc aaaatagata gctgagtaca
     1021 aaatatttca taaaattaag caatataata caagagaatt ttgacattat ttatatatat
     1081 ttaccgtgta caaatagcta aatttaggat taagattgaa aaatatctta taagtctgag
     1141 cttatataaa tccgtataag atgtatttaa tcgaaaaatt ttattatgga tattgcttgt
     1201 ctatattctg agtagcaagg ttttatcatg actataaaaa agttgccaca atcagaatta
     1261 aagataatga aatttatatg gaaatcagat tccaaagtaa catctagaga tattgtttta
     1321 ggaatggagc aaaaatatca atggaaacaa acgacaactt tgactctttt atctagatta
     1381 gtagtaaaaa gatttctaaa ttctcaaaag atagataaat atacgcatta tgaagtttta
     1441 attaaagaaa aggaatacat aggagttgag acaagagatt tttttagaaa tattcatgat
     1501 tcttctataa aaagtttatt gttgtcatta catgaaaata ttaatttgag caaagatgat
     1561 atcttactta ttgaagagtg gataaaaaat ctaaaagaag aggagaaaga cgtataagca
     1621 aacgtatttc ttatagcatc aatataaagt gtatgtaagt gtatttatgt aatatattag
     1681 tctacttaag taatatgaca aatgattata agtggattat gatactataa tgtgaatcta
     1741 aataaattaa ataaaaatta atagtggata ttctatttaa ttaaatttaa aaagctacct
     1801 ttacttataa gtataattaa ggtagctttt taaatagagg tagtgtaatt aaagagaatt
     1861 tttaaatata aattgtgaag ctttaatata gatgataata tatattttgc aaatatgaca
     1921 cagtgttttc gtatttaaga tgataaaata aataggaatt aatgatgata ttgaaaaaat
     1981 aaaaataaag gattaatata agatattaaa gagatagaga taaaagtgtt aacagaagat
     2041 attgaaaaaa taaaagattg atataagata ctgaagaggt agaaacaaaa gtattaatag
     2101 aagatattga aaaaataaaa acaaaagatt gatagaaagt actgaataga tagagataaa
     2161 agtgttaata gaagatattg aaaaaataaa agattgatat aagatactga agaggtagaa
     2221 acaaaagtgt taatagaaga tactaaaaaa gtaaaaataa aggattaata aaaggtactg
     2281 aagagataga gaaaaaaatg ttaatagaag atactgaaaa aatagaaata aaaaattagg
     2341 aggaaaattt atgaaatatc aggtttcatt attagcagtg aaggatattg aagtatcaaa
     2401 aaaattttac aaagaattat ttgagcaaga aattgaattg gacttaggta aaaatgtaac
     2461 atttaaagga ggatttgcta tacaagaaga cttcgcatgg cttacaggga ttaatccaga
     2521 tacaattata caaaaatcaa ataatatgga actttatttt gaggttgatg attttgacga
     2581 atttattgaa aaactcaatt attataaaga tgtagagttt gtccataagc cactaaaaca
     2641 tgaatggcaa caaagagtag tgcgtattta tgacccggat aagcatatta ttgagatagg
     2701 agagtctatg gaagttatag ctagaagata tttatctgag ggatactctg tagaagaaac
     2761 ttctaaaata attcaacatc caattaaatt tgtaaagcag tgtgaaaata gtaaggaatg
     2821 attgtttaaa ttataattat ttagtagaaa ttaattgtaa tagaactaat tcaagataat
     2881 atataggact agtatttgac aaatgtcata aattaaatta aaataaagaa aatattaata
     2941 aagaaaaaat aaataaaaat aaggggaata ttaataaaga aaaaataaat aaaaataagg
     3001 ggaatattaa taaagaaaaa ataaatataa tattaaaata tattaataaa tttgaatatt
     3061 ta
//
LOCUS       DAEMGG010000228         2950 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEMGG010000228 DAEMGG010000000
VERSION     DAEMGG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.231"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            418..2229
                     /locus_tag="KPK24_003725"
     CDS             418..2229
                     /locus_tag="KPK24_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2301351.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2317..2898)
                     /locus_tag="KPK24_003726"
     CDS             complement(2317..2898)
                     /locus_tag="KPK24_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301352.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1115 a          304 c          416 g         1115 t
ORIGIN      
        1 aaaggaatat atttacataa tatgataaaa taaattcaaa gagttaaatt gtaggtaaat
       61 agtattttaa ttatttatta aaatatagat tatgtatatg ccaaatttaa aaagagacat
      121 aaagacatta tacaaatata ttattggggt gatattttgt aagaaaagaa gtgatgcagt
      181 aaattaattg agtaatttaa aaatgattta atgataatgt tgatgaatgt ttaaaaagaa
      241 attttagaaa aacgtatatt gctgtttgtg ttggaggtga tgaatttgta gaatttatag
      301 ggtataaatg attaaactat ttaagataaa tacatatcac tgtaaaatat tgataatcat
      361 tttttataag agattgatta tttgcaaatt atatatatta caaatgaggt aacaataatg
      421 aagaaaaaat atgatttttt aatttattct gctatttggc tagttattat tattcttttt
      481 atattttctc tatatcacag tatagagcat attaaaatta taagtcacac tggtactatt
      541 cgcagtgaaa ctcaaaaggt tgttaagcaa gaattgaata atgaaagaaa tgatgactta
      601 ataaaacgac tggataatat tctgataaag ctaagaacag gtaatggaga aaatggattt
      661 caaagatgtg ataataaaga atttcaacaa aaactaaatc aaatggattc tatgtgggaa
      721 tctatgaaga aagaaattat aaaagttaga aatggtgctt caggtgataa attatataaa
      781 ttgagtgaag agtattctgt gttgtctaat caaattgttt ttatatctga aaaacactct
      841 aatgcaaaat tgtattcttt tgctactgca ctatttattt atcttatatt ctcaacaata
      901 agtcttttaa tttgggaata ttataataaa aaaaggttta aaagaatatt ttatactgat
      961 aatttaacaa aaataaaaaa tcaggttgca tttgaaaaca gagcgattga aattttgcac
     1021 aatgcatcta acaaagagta tgtactgcta aatattgata ttgacaattt taaatatatt
     1081 aatgatacac atggatatga atatggagat aaagttctaa taatagttgc agctgcactt
     1141 tctaaaactt ttaatatcaa agaaacttgt gcaagaattg gttcagataa ctttgtaata
     1201 cttgcaaaat atagagattc tcttttagag gatattcggg aaatgctaac taatgctata
     1261 atctctgaat tagatatgaa tgtaactcaa actatatctt attgtatagg agcgtactta
     1321 gttgaaattg ataatttagg ttataaatcc atcaattcta tgatggataa ggcaaatatt
     1381 gcacacaagg taagtaaaac aagaggaatt tcctctacag tatggtataa tgagaatctg
     1441 ttaaaacagt tacaaatgga aaatagtatt tataattata tgtataaagc gatagaaaat
     1501 gaagaatttc atatgtattt gcaacctaaa tttcaaatat cttctttaaa tgttgtgagc
     1561 gcagaagctc ttgtaagatg gttttctcca gaacttggtt ttttatcacc agatgaattt
     1621 attccattgt ttgaaaaaag tggatttatt attgaattgg acttttatat gttaaaaaaa
     1681 gcgtgttcat ttgtaaaaaa aacgtttatg aaaaaaaatc aatatacata tccgattgca
     1741 gttaattttt caagggttac tatttaccaa aatagctttt atcaaaggtt tttagatact
     1801 gttaaagaat atgaaattcc ttttaagtat atagagatag aggtaactga aagtgctttt
     1861 aatgaaatat ctcaaccagt tatttcgatt ttagaggagc ttaaaaagtt aggattttta
     1921 atttcgatgg atgattttgg atcaggatac tcttcattaa gtcttttatg ttctctatct
     1981 attaatggat tgaagttaga taaaagctta cttaaagaaa catttaaccg ggagaaagta
     2041 tatagtatta ttcagtgtat tattgaaatg tcacatcgta taggcatgtc tgttgtatgt
     2101 gaaggtattg aaactaaaaa agacttggaa tttttaaata cagttaaatg tgatgtagga
     2161 caagggtttt atttttctaa accgatagaa gaaaaagaat tttttaacaa atatgtaact
     2221 aaaaaataat ataattattt ttatcagtct atacattgag tcattttata tctcgttaaa
     2281 tgactcaatg tactgttatg tattttaaaa tataaattat ttaaaacgtt ttttatactt
     2341 atatagaaat tcttctatat tttttgattg ttctcttatt tcaactgctt ctatatctag
     2401 taaataaagt atagaatcat ttgtaagttc aacatattca ccatcttctg aaccgagtgc
     2461 atgtctctca atttttaatc ctttttcttc ataatatttt tttgtttcta tatataatga
     2521 ttctcgcttt tgtatgtagt ctttcacttc attatttgga aagttatatg aatccactaa
     2581 tgtttcgcca tttataaaag ttttctcggt agcaactttt tcatcccaac aatggatttc
     2641 ttcccaagta aaaaatatct cagttccatc tggaatgaat gaaaacctgg taataatata
     2701 taaataatta ttgaaattta cttcataata ttcaggaata tcttcaaatt ccatatagta
     2761 atttgtataa ttaaaattac tattatcttt ttttacaaag ggtatttttt caataaattt
     2821 atatattgat tgttcatttt taaaaattcc tatatcataa cttttttcaa attgattaat
     2881 ttttaataaa tacaacattc tttctccttt tattcaaaat ataattttta taaatttact
     2941 aatctttagc
//
LOCUS       DAEMGG010000229         2933 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEMGG010000229 DAEMGG010000000
VERSION     DAEMGG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.232"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(764..1315)
                     /locus_tag="KPK24_003727"
     CDS             complement(764..1315)
                     /locus_tag="KPK24_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301353.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1506..2468
                     /locus_tag="KPK24_003728"
     CDS             1506..2468
                     /locus_tag="KPK24_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG2301354.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT         1138 a          295 c          463 g         1037 t
ORIGIN      
        1 gaaaaaaatt ataactttat tttattagca atctttttta tattgctgtt aattcaaaat
       61 ggagttttaa aaacaggtta tcaaaataga gctacatttt atcaagataa ttattttaat
      121 ggagaggata aaaatgataa gataaaattt atgactataa agatttttat atttttgtca
      181 ttaccattat gttttaatat actattcaat tatttaacct cttaaattat atttagatat
      241 aggagtctta tgataaaagt aagcttatgt gttatttgta gtaaatataa agtgcatata
      301 gtgataaaac aaaagtttca tataaatgaa gaaaaaaata taaaataatt tatttaacta
      361 cttaaaaata aactaaaagg gataataaaa tagcttttag tttatggtaa tagaggtatt
      421 taaaaagagt gcataagcac tccaattgtg agaaatacat acacaaatca ttattacaac
      481 aatgaaaaat gtacatataa gagacgtttt gtatttgttt taagtgataa tattttaata
      541 ctaactaaat aattatcaca atggtaaaaa agaaaaagga gtattttgta ctccatttgt
      601 caaaaatata aaacttttat ataagcttat tataacataa ttgataaaag tgtaaagata
      661 tataatgaat ttataaagaa aaataaaata aaaaagataa atcctaatca aaggatttat
      721 ctttttgtat agaaaataat tattctatta ttaaatggca tggctaaact ggataataat
      781 ttattattac tgctttcaat gttttttctc cttgattatg gtatgtatga attgattcag
      841 ctgaaaaaca aatagagtca ttttctctaa gttgataaat agagtcattt acttctaatt
      901 ttaattgacc ttctataatc ataatatatt cctgtgattt ttcagaatga ccaacagatg
      961 tataactatg accttcttct aattccattt gaaataattc gaagtttcta gttggtgtat
     1021 tgggataata acaataaaga cgataatctt tattttctga aacttgtaca tctatatcag
     1081 tttttgatac aataaaagtt tcattttgag gttgttccag tattgcagta tatggaacat
     1141 ttagtccact tgcaattttc caaattgtgt ttactgttgg gtttgagtct cccttttcaa
     1201 tttgagaaag cattacttta cttacgccag ataattcagc aagttgacct agacttaagt
     1261 tacgttcatt acgtaatcta tttaaatttt tagcaataac agtattaata ctcatagtag
     1321 ctccttaaaa tgtaaattat tgacaaatat aatttacgaa tgtataattt atttaacatt
     1381 atttataatt tacaaatgta taatataatg aacgttaaat acactgtaca tccgtaatat
     1441 aaattatact gcataaagag ttgtttttca acggatgata gttgagagat taagggggaa
     1501 ttgtaatgcg tgtacttaat aatgttacag aggctatagg caatactcat atgttacatt
     1561 tatccaaaat agcagatgaa tttggagttc aaggtaatat ttatgcaaag atggaatact
     1621 taaatccggg atttagtaaa aaggaccgtg tagcattaca aatgattacc gaagcagaaa
     1681 atgcaggttt attagttcca ggtcaagcag ttgtggaatt aacaagtggt aatactggaa
     1741 caggactagc aatagcttgt gcatgtaaag gatataaatt tatagcgtgt atgtcaaaag
     1801 gaaattctat ggaaagagca agaatgatga aagctttagg tgctgaagta gttcttgtag
     1861 accaaatgcc taattcagtt catggacagg tatctggtga agatttggca cttgtagaaa
     1921 ttgaggctca aaaaatagca aaagaaagaa atgcatttag agcagaccaa tttaatttag
     1981 aagcttgtaa tcatgcacat gaaaagtata cagcagaaga aatgtgggaa caattggatg
     2041 gaaatattga tgtatttgta gattttcttg gaagtggtag tacttttgca ggatgttcaa
     2101 aaacacttaa aaaacataat tcttctatca aatgttatgt tgttgaacct gaaacagcag
     2161 caatttattc aggtaaagaa ataactgacc aagggcataa aatacaaggt ggaggatatt
     2221 ctatggattt gccattagta gacaagaaac ttattgatgg ttatattcaa gttacagacg
     2281 aggaggcaac tgatgtagca agaaaattag ctaaattaga aggtgtattt gcaggatttt
     2341 cttcaggggc aaatgtatgt gcagcaatta agttgttaaa gagcagtgaa aaaggaaaaa
     2401 atatagtttt aactcttaat gatagtggtt taaaatattt aagtactgat ttatatgaag
     2461 aattgtaata atagcacaat agttatgcaa tgttttttaa attaagtata tagatttaaa
     2521 ttataaaaag tatctcaatg taaattttga aagttataaa ggagatactt ttttattttg
     2581 taaatatgga gtatcctact tataagtatt aagctatata gatattgatg attagctatg
     2641 caataaaaaa gagtatgtgt ttttgcattt atacatcata acttggaata atacatatta
     2701 atgttctaaa tgtatttaaa atatatgata aaattagaac aaatagatac aaggagataa
     2761 aaatgcaaaa gagaatttta agaaatttta tatttacggg taaaaatatt ttaagtaaac
     2821 ttgtttttat aataaaatat aattttagta cattaatttt atttgaatta tttcataaag
     2881 gattagcctt attttttata tggccatcta ttagatatat atttgatatt tta
//
LOCUS       DAEMGG010000230         2885 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEMGG010000230 DAEMGG010000000
VERSION     DAEMGG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.233"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            343..1620
                     /gene="hflX"
                     /locus_tag="KPK24_003729"
     CDS             343..1620
                     /gene="hflX"
                     /locus_tag="KPK24_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG2301355.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVDVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     MSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1853..2350
                     /locus_tag="KPK24_003730"
     CDS             1853..2350
                     /locus_tag="KPK24_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2301356.1"
                     /translation="MDAETLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2400..>2885
                     /locus_tag="KPK24_003731"
     CDS             2400..>2885
                     /locus_tag="KPK24_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG2301357.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KE"
BASE COUNT         1198 a          253 c          515 g          919 t
ORIGIN      
        1 taaatataaa atgataatta catataagtt aattaaacta atgtattttt aatatatatt
       61 tagaatttta atccttctta tgatataagt agcttgtttt gtattactta gatttgtaag
      121 gagttttttt attgaaaaaa ctgcataata tatttgggag gaggtgtaag aaagtgattt
      181 tttatataag attgagaaaa taatgcacga ttatggttat tataatagta aataagaagt
      241 gtcatttaag gataagataa aaagcaaaat tcttgatgaa tttaattata gagccataat
      301 ctcgtgctat agattaaata aaaaatagca gataaggaga atatggatat ggcgaaaaaa
      361 ggaataacag taggattaaa cataaataat aaatctgagg actttaatga actaatgatt
      421 gaacttgaaa acttatgttc agcatgtgat attgatgttg tagggagtat aactcaaaat
      481 gcaaagcagg ttaatagagc attttacatt gggacaggta aagttgagga aatattaaac
      541 ttaataaaga aagaaaatat agagatagtt atattttaca atgaactttc tacatcacaa
      601 ctaaaaaatc ttgaagaaaa gcttaattgt gaaattatag atagaacggc attgatactt
      661 gatatatttg cacaaagggc aaaaactaga gaagctaaac ttcaagtaga agtagcaagt
      721 ttaaaatata tgttaccaag acttataggt tcaaatgaaa atttaggacg tcaaagtggt
      781 ggagttggta ctaaaaatag aggttctggt gagaaaaaat tagaacttga ccgtagaaga
      841 atagaagaaa agataacatc tttaaatagg gagttggatg atttaaaatt ccaaagagaa
      901 actcaaagaa gtatgagaag aaaatcaaat cttccaaatg tagctctagt cgggtataca
      961 aatgctggta aatcttcaat aatgaataaa ctagtagata tatttaaaaa ctctgaagaa
     1021 aagaaagtgt ttgagaaaaa catgttattt gctacacttg aaacttctgt cagaaatata
     1081 gttcttgcaa ataacaaaga atttttatta tctgatactg ttggttttgt aagtaatcta
     1141 ccacatgact tagtaaaagc atttaggtca acattagaag aagcatgtga ggcagacgta
     1201 ctgttacatg ttattgatat ttcaaatcca agctataaaa gtcatattaa agtgacagaa
     1261 gatactttaa aacaaatagg tgcagatggt atacctatga tacatgtgta taataaaatt
     1321 gatttaatag atgtagacgt attggataga attttagata gtattgataa agaaggtata
     1381 tttgtttcag ttaaaaaaga tataaatata gataaaatga taaaatgtat ttgtgatagc
     1441 atatttaaag attatgtaag atgtaagttc ttaattccat atgataaagg tcatgtagtt
     1501 tcttatttta acgaaaatat gagcattata aatacagaat acagagaaga tggagctatt
     1561 ttagatgtag aatgtagcaa tatagaatat aataagtata aaaagtatgc tttagagtaa
     1621 attttgaaaa acttattata gcctaaaata tatttaattt ataatttatt ataaattgat
     1681 tgtaaatatg attttatgaa acggtatagc cataatgcta tgccgttttg ccatttataa
     1741 taaaaataaa tttattgaca taatagtaac taattactat aatataaagt aattagttac
     1801 tatttaaaat tatcaaaaaa ttaaaatcta agataaagag aagtgtataa taatggatgc
     1861 agaaacttta atgaaaaaat atatggtaga tgatgatagt agaaaacaag ctatatttgc
     1921 aagtatattt gtaatgcaaa ataaacttca aactacatgt gataaattag acccagaatt
     1981 aacaatgaaa caatggttac ttttagctat tgcggactct gtagatgaaa ctcttacatt
     2041 aaccaaactt ggagaattaa tggggtgctc aagacaaaat gtaaaaaaac tagctattgc
     2101 tttagaaaag aaaggtttta tacaaattga gcagaatgaa aaagattcaa gagctatctg
     2161 tcttgtgaca aaagatagag tgcaagagta ttctaaaaaa gtaagaggta tgcaggaaaa
     2221 agtattacag cttttatttg aagattttac agagaaagaa gtggaacagt tattttttgg
     2281 aattatgaaa ttaagtatag gaattgaaaa tgtgaaaaat tatgtagaaa ataatgacgt
     2341 tggagaataa gcaagtatca aatcaacttt gtatgacatc agagaggggg ataatttata
     2401 tgaaaaatgt agtagtgatt tataagagta agtatggttc aacaaaaaaa tatgctaaat
     2461 ggctttcaga attattgtct tgtgatattt ttgaaactaa gaatattaat atagaaaagg
     2521 ttatgaagta tgatacgatt attttaggag gaggtcttta tgcttgtaat atagcgggaa
     2581 tttctttctt aaaaaaaaat tataaagaat tgcagaaaaa aaatttagta gtatttggcg
     2641 taggagctgc tccatatgat gatgaagtaa taaagaaatt aagagaagta aattttaagg
     2701 atgaattaaa caaaattcct tgcttttatt gtcgtggggc atgggatgag acaaaaatgt
     2761 cttttataga ttatactctt tgtagttttt ttaaaaaatc tatagctaaa aaagaacgtc
     2821 aaggtcttaa accagcagaa gcacctttat tgtctggaga agcatttgat tggtgtaata
     2881 aagaa
//
LOCUS       DAEMGG010000231         2876 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEMGG010000231 DAEMGG010000000
VERSION     DAEMGG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.234"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      <1..67
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            150..635
                     /locus_tag="KPK24_003732"
     CDS             150..635
                     /locus_tag="KPK24_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2301358.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     gene            991..1389
                     /locus_tag="KPK24_003733"
     CDS             991..1389
                     /locus_tag="KPK24_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301359.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILFIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    1824..2087
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2238..2789
                     /locus_tag="KPK24_003734"
     CDS             2238..2789
                     /locus_tag="KPK24_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG2301360.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT         1243 a          259 c          429 g          945 t
ORIGIN      
        1 ctaatgacgc aaaactatag ggactaaaat ctttatgtaa taaaggtcat gtcagccagt
       61 tgccaaaaag ataataattc tttttgttaa aagtattttt agagttatat attttaataa
      121 cttaatattt ggcaagagga gggcttaaga tgatatctga attaatgtta aaaaattttc
      181 gcaaatctag catcaatttt aagactaaaa ttaaattgaa tttaagagaa aaaccagaca
      241 taaatagttt gaaattaaaa agtataccag aaggtaaaat tgttaagtta aagtgtgtag
      301 atggaatatg ggcagaagtt gagagtaatt atgacaaagg ttggttactt tataaatatt
      361 tagaaagatt aagtaatgct aaaaacaata gtaatgatac aataaaaaat agaaaaaaga
      421 attacatagg aaatataaga acaaatggat taagtttaga acttagaaat gatagaacat
      481 tagaatctaa agttataact acaatccctg atggttttaa agttgaagta tgttatagcg
      541 ttggaaaatg ggcaagagtt aatatcaata aaaatgaaaa acgatattct ggatatgtat
      601 ataatcaata catagaagag catttaagtt tataatacga tactagcaag ctttaaatat
      661 attaataaat cgcaaaaaag aatcccatta atgggattct tttttgtaga aacattataa
      721 cagaactaaa gagaataaat taactaattt atttttatta taaagtaatt ataaatggta
      781 aatttaattg aaagtagatt taacttatac tttatacacc aagtatcata aattaatact
      841 actttgaaaa atcttaaaaa aagtaaaagt tatgataata aaaataatat taaagataat
      901 aagatatata agaaacttga aaaaatttgt aatttttagt tatatatata ttataatgaa
      961 ctagttaaaa atttttatga gggggaaaat atgaataaga agaaaaaatt gattgggata
     1021 gctatatctt ttctacttat aatcagcatt ataaatccat tacaagtaga agcagaagct
     1081 attattgagt ctagtgtata catttcttca ataaaagaga attatacata tgataatact
     1141 aaatttaaaa ctattaaatc taaaagtaga cataaaagta ggaaatataa taaaagtaaa
     1201 gtaaagttga aaaaacgaag tataagaact aaaaagtatg gatatcaaac tataaataaa
     1261 tataaaagac aaaacgcaag aagacataat atcctaacaa aattaatatt gttcataata
     1321 gtcatatttt tggttatagt tttagtagtg ctaatattta ttaaaagaaa gaagaaaaaa
     1381 ttttactaaa aataattaaa agaatatcat ttttttaatc attggtatct atttaataat
     1441 atgatgtaat taaaatggag taatattctg tttcaaagga ggcatataaa tacaggaatg
     1501 agtattaaat gatggttaat taagtaaatt tattgataaa tgtacttaat taactttgaa
     1561 gaatttaatt cctcatgaat caaaatataa aattacatca acagttattc acaaaattaa
     1621 tataagaaga attagagcca aagagcagac tattagacca ttaaagtaat tattcataga
     1681 attttgtata tgaaaagaaa tgtatagtat aatttacttt ttttataaaa aatagattac
     1741 aaaatatatt ttttataaaa aggtattgac attttttaga aaaaagaata ttattatttt
     1801 aaaacaaaat tcaatacaat aaattaaatc ctatgacaag gaagagtagt taaatttagg
     1861 ttcagtatag agagctgagg atggtggaat cttagcaaga aaaaatttga tgaatggccc
     1921 ttagaggaat aaattgaaat catagttttg agagtagact ttatcgtcag tcgcacgtta
     1981 tagcgaataa ggtatgtaag tacctgataa tgagatgtat gaaaattgaa ttttatatta
     2041 atttttatat aatttaggtg gcaacgcgga taatctccgt cctattagtt taggacggag
     2101 ttttttattt tactgaaaat ttaaaagata gaaggtggtg agaaagatgt tatgaatttc
     2161 aaaagctaaa gatgatgata tagtaggttg gcaaaaaaat aaattgaaaa ttaaaaaaat
     2221 aggaggaagt acaaaaaatg aaaacaaata caaaaaaatt aactttaaat gcaatacttt
     2281 tagcgatggg attattaata catcaaatca caccagctat aggattacca atgaagccag
     2341 atgtaccact agcaatgtta tttgtaatat tggttttaaa tagagatgat tataaaactt
     2401 gtttgatagc gggtatagta acaggaatat ttacagcact tacatcaagt tttccgggag
     2461 gtcaaattcc taatgtaata gataaaactc ttacagcaaa tatagtattt ttattaatga
     2521 atattagtta taatatgcct tttataaaaa aattagctaa gaaaacacaa gatactgtag
     2581 tagttgcaat aataatgcca ataggaactc ttgtaagtgg aactatcttt ttattagcag
     2641 ctcaagctat agttggtctt ccaggggctt catttacagc tttattctta gcagtagtat
     2701 taccagctgt tttaataaat ctagttgcag gaatattttt attcaagatt gttagtctat
     2761 caataagaag agttagttat caagcttaaa cacgaaataa tattttactt tataaagcat
     2821 tataataact aaataacata agaataaagt agattataac tattttattc ttatgt
//
LOCUS       DAEMGG010000232         2642 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEMGG010000232 DAEMGG010000000
VERSION     DAEMGG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.235"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            375..668
                     /locus_tag="KPK24_003735"
     CDS             375..668
                     /locus_tag="KPK24_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2301361.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            649..1290
                     /locus_tag="KPK24_003736"
     CDS             649..1290
                     /locus_tag="KPK24_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG2301362.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFVYSYLMYKISGKNN"
     gene            1534..1866
                     /locus_tag="KPK24_003737"
     CDS             1534..1866
                     /locus_tag="KPK24_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG2301363.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            2408..2572
                     /locus_tag="KPK24_003738"
     CDS             2408..2572
                     /locus_tag="KPK24_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301364.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1010 a          226 c          406 g         1000 t
ORIGIN      
        1 gatgttttta tgtactttta tacagtatat ttctcgtttc attcttttct atttaaatag
       61 gacttaacta ctttttaaat tgggtttagc tacttttaat ttaaaattaa aagtattgtt
      121 atatatataa gtacctcagt cattaggttt ttgctttaac aattatggtg caatacactt
      181 ttgttaagtt aaaaaaatta gataaagtaa gattaaataa aagcgactta agtaaattga
      241 gaatcttaat atgaaaattt tcaataaata tttagaaata actatttaga aaaaattcta
      301 aatagttatt gaaataaaat ggatgttatg ttatattcta tttagatgaa attctaaata
      361 gcgaggtgat ggtgttgggg tttccagaaa cattcaaagc tctttcagac cctataagaa
      421 gagaaatatt ggttatgttg agggagaata aaatgtcagc aggagaaatt agtgaaaaat
      481 ttaatatgac gaatgctaca atttcttatc atctatctca actaaaaaag gcaggactca
      541 tatttgaaac aaagtataag aactttattt tctacgaaat aaatatttca gttttcgaag
      601 aggttatgct ttggttttcg caatttatgg agaagggaga aaaagataat gaaaaaagcg
      661 ataaataaac aatttatttt atctacttta atatgtttta taccatttat tgtatcaata
      721 tatttttata atagattacc aaatgaagtt gcaattcatt ttgataatta tggaaatcca
      781 gataattatg ctccaaaggt tatagcagct tttggtgtac cattgttaat gctatgtatc
      841 catttgtata catggtttag acttgagaat gagcctaaga aagctaattt ttcaaatgtt
      901 atacaaaatt tatcaaaatg gggagttgct atactttcag taattataca aataatgatg
      961 gttttatatt caacaggagt tagtttggag tctaattttt atacaggatt aattttagga
     1021 ataatagtta ttttatttgg aaactatctt cctaaatgta aacgaaatta ctcagtagga
     1081 ataattttac catggactct taatgatgaa gataattgga ataagacaca ccatttagct
     1141 ggatggattt ggcttattgg tggaatatta ttaattatta atgcatttat taatattcct
     1201 ttttataata tctttgtaat ctttgtaatt gtaatactac catttgtata ttcttatttg
     1261 atgtataaaa tttcaggtaa aaataactaa tataattaat cactataata atgtttggat
     1321 aagaataata tatttcagaa ctgagataga taataaagaa attactgaga tttctatttt
     1381 agttctgaaa aaatcttact tatatgataa cttattgaga tttcaaaaaa taatataaat
     1441 taggctaatg aacataaaat gaaaatttgt atttaatatt gaaaatataa gtacagtgtg
     1501 atactcttat aagaaaaaaa taggaggtag aagatgaata ctagagaaat gaaagaatat
     1561 aggggatttt acaataaaaa attaagtgaa acagatgagt tttttagagt atttggaagc
     1621 cttgatgata agacttattc agaaggggct ataagtaaaa aaaataagga attaatggga
     1681 ctagctattt caattttaag ccgatgtgat gagtgtattt gttatcatat tgagggttgt
     1741 attaattctg gagcaagtaa agatgaaatt atagaagcta taaaaatagg tgtaattggt
     1801 ggagggtcta ttacatatcc aaacgcaaga tttgcaataa aaattttaga agaatttaat
     1861 atataatttt gatacacgta gtttatttag actaataata atagtctatt taagcctttg
     1921 aaattaagct ttattttcta agttcacttc ttattaagtt tttagaattg catatataaa
     1981 tataaatttt taaataatat agtagtttta aattatttac atcatagatt gtattgagaa
     2041 tagtatattg tcattattaa atcttttata cattttttat tttaaataaa ttgaatatta
     2101 aggtgattat atttacatat ttgtattttt atgaactaga atattcttta taaattcata
     2161 gcagttttaa tagataactt tttcagatag attaaacaaa gttttattta ctgtattcta
     2221 ttatcagttt cctacaaatt aaattgtcta tttttattta tagagtagaa taaactgttt
     2281 ttgttaagaa ttttgaaggt aaagctagtt taaaaaaata aagggactaa tttgtctaac
     2341 gtttattggt gaaaaataag tctatagaag ttataataaa tatataaaaa tgtatgagag
     2401 gtgttatatg tttggtaaac caaaagaaag tgttagattt ttagacttta atgatgtagc
     2461 agaaaagttt ggagagaaaa aggctgttgc aatttttgat gccatagctg gaaaaaaatc
     2521 taaagaacat attatcaaag atatagaaaa attagtaaaa aatgaagttt agatatttat
     2581 aaggtatatt gagcttgagg gtagctcttt ataccttctt ttttatgatt tttagataat
     2641 at
//
LOCUS       DAEMGG010000233         2640 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEMGG010000233 DAEMGG010000000
VERSION     DAEMGG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.236"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(373..1128)
                     /locus_tag="KPK24_003739"
     CDS             complement(373..1128)
                     /locus_tag="KPK24_003739"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG2301365.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHDTLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1568..2557
                     /locus_tag="KPK24_003740"
     CDS             1568..2557
                     /locus_tag="KPK24_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG2301366.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          987 a          316 c          390 g          947 t
ORIGIN      
        1 taggataata tttaataggt ttatatgatg aaaagattat aaaataataa acaatatgat
       61 tctatatacc aataaatagg ataatattta ataggtttat atgatgaaaa gactataaaa
      121 taataaacaa tatgattcca tataccaata aataggataa tatttaatag gtttatatga
      181 tgaaaagact ataaaataat aaacaatatg atttcatata ccaataaata agataatatt
      241 taagaaaaat gattatattt aaaaggtaag cataaaattt ataaactttt agaatagaaa
      301 tctcaatcaa ttagatattc aaattgatga atatgaatac ctaaatagat tgagatttat
      361 tatgtacaaa acttatccta atatatctga cattttataa agcccagatt gcttaccaat
      421 taaaaattta gctgcaacta tagagccgtt tgcaaatact tcatcagatt gagaatgatg
      481 gcttaatgta agaacttcat tatcacctgc aaatacaaca tcatggtcgc tgattatatt
      541 gccacctctt atagagtgta ttccaatttc atttggtttt cttttacaat ctctaccata
      601 tctaccatgt gtattttcac tttcaggcaa gacttctcta attgcattag ctatcatagt
      661 agcagttcca ctaggggcgt cttcttttct attgtgatgt ttttctatga tttcaatatc
      721 aaaaccttct aacatctttg aagcatcctt aactaatttt attaatatat ttactccaag
      781 agatgtattt gatgataata atataggaat gttttttgat gcttcttcta ttttatttaa
      841 gtcattttcg tcatatccag tagtagctaa tactacaggt gtattatttt ttagagcata
      901 tgacaatatt ggctctaaag tatcatgatg tgaaaagtct ataattacat cagttttttc
      961 agttacatca gtcattgttt tgtaaagctt tgtatcacta tgatgacctt tttcatcagc
     1021 agacctagct acaccacaaa ccaactctaa ttcattatct ttttttacac attctgctaa
     1081 aactcttccc attgttccat tatagccact aattataact ttcaacataa attcctccta
     1141 tgaaattaat aaaaatttgt tatatttcta taagtttatt ttatcactaa tataatttat
     1201 ataaaataat attagtgata ataagcttac aatttatctt acattaaaaa atttaagtgg
     1261 atttttcaaa atatagataa atttcttttt cattgtttaa atgataaaaa tatatttgac
     1321 ttcataccaa aaagagtgcc ccatgaacta aaaagttaat tttgaaatac catattcata
     1381 tatcatatga tttatttgga aattaatatt aatacattaa aataactaaa tacatgtatg
     1441 cataaagatg tataaaaaat atataaacat atagtattaa ttttaaattc aacaatttaa
     1501 ttgttaaaag tgttattatt aaattagaaa tatcttaact taatatactg agatgggagt
     1561 gatagatatg ttaaaagaag aaaaaactta tcaagagaaa gacgcaaaag tattcaatgg
     1621 agtggcagct ttgataatga ttttattatt tttagtagtt tcaataggta tcatggtttt
     1681 tgggatagta aaacttgata gtggatttac tggaataggt tcacttatgt taattttggg
     1741 acttgtattt atagtagtag attttatcct attttttggg ctaaaaatga ttaatcctaa
     1801 agaagctata gttctagttt tgtttggtaa ctattatgga actataaaaa aagaagggta
     1861 ctattgggtg aatccatttt gttcagcaat aaacccagca gtatcaagaa ttaatgtatc
     1921 gaaatcttca tctgatgaaa aggtagatat atcaactaat tcaaggggca aaaaagtatc
     1981 tttaaaaaca atgactctta ataatgaaaa gcagaaagtc aatgatttat tgggaaatcc
     2041 aattataata ggagtggttg taatttggaa ggttatagat gcaacaaagg cagtatttaa
     2101 tgtggataat tataatacat tcttatctat tcagtgtgat tctactattc gcaatgtttc
     2161 aagactttat ccatatgatg tatctgaaga tggagatgaa aaatctcttc gtggtagtag
     2221 tcaggaaata gcagatagat taaaagatga attacaatca agggttgaca ttgcaggaat
     2281 tgaagtgtgt gaagtgagaa ttactcatct ttcttatgca ccagagattg cagcagcaat
     2341 gttacaacgt caacaagcag aggctataat agcagcacgt aaaaagattg ttgagggggc
     2401 tgtaagtatg gtagagatgg cattgactaa tttaagtgaa aataatgttg tgactttgga
     2461 cgaagaacgt aaagcagcta tggtaagtaa tctgttagtt gtgctttgtg gcaataaaga
     2521 tgcacaacca gtggtaaaca gcagttctat ttattaaatt ataaaaagtt taatagttta
     2581 ttttattgat atatagaaaa tttagtagtt cattttatta aattatataa agattaatgg
//
LOCUS       DAEMGG010000234         2560 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEMGG010000234 DAEMGG010000000
VERSION     DAEMGG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.237"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            636..2432
                     /locus_tag="KPK24_003741"
     CDS             636..2432
                     /locus_tag="KPK24_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG2301367.1"
                     /translation="MKPTMEILTKLQENSKKNHDEVFTRLYRYLLRPDIYYVAYQHLY
                     SNKGAGTKGVTDDTADGFSEIYIENIIEALKNEMYQPKPVRRTYIKKSNGKMRPLGLP
                     VFTDKLIQEAIRMILEAIYEPIFSDYSHGFRPARSCHTALAQIKKEFTGARWFIEGDI
                     KGCFDNINHAVLVEIINQKIKDARFLKLIRSFLKAGYMEDWKYHKTYSGCPQGGIISP
                     ILANIYLNELDRHVMKIKKEFDVATKARYTPEYTKLVGLRQRLHNKIKNSNGIEREKL
                     IEEYKSATAQMLKLPAKQCDDKKIKYVRYADDFLIAVNGNRQDCEKIKQELTEFISTT
                     LKMELSQEKTLITHSNTPARFLGYDVRVRRDQQIKPKGKFKTRSMNNKVELSIPFKDK
                     IEKFLFSNGIVKQRSDNGKLEPIHRPQLLNRTDLEIVTIYNAELRGICNYYGLASNFN
                     KLIYFNYLMEYSCLKTLAGKHRSKVSKIRAMYKDGTGKWAIPYETKTVIKKMYFANYA
                     DCKGKKFTDIVPQTAKNYSHDVTTLESRLKAKICEVCGCTESDRYEIHHVNKVKNLKG
                     KSEWEKIMIAKRRKTIVVCHKCHMAIHHGEKK"
BASE COUNT          977 a          439 c          533 g          611 t
ORIGIN      
        1 ttagaagtag attaaagaaa gggggacaga aaaaaaaggc actaccccgt aagataacac
       61 gggaaaccac ctgcctaacc gaaaggtgaa aactgtacgg gaaaatagca cggcaggaaa
      121 gcagaaagtt gttcaaagac aatagagcac gactgaactg caaggttaag tgaatatgag
      181 gataaaacta gatttgttga atgtgagttc caaacctctg ttacgtgtgg acacaggaaa
      241 cttgtctgaa acctgtggta tgagtaacgc tgaaaataaa tgcctttcag tgggttatca
      301 attaactcat acggtacgct ggagaaccag tagtaacgga acgaaagcat atccgacaat
      361 tcacaccagc ctactacttt cgtcaactgg agattaccta aaccagaatg cctacaatgg
      421 ctatgtgtaa tgctcttgta gtgataaatc acaaggctat gctaagtgag atgacgctga
      481 aaatctggca aggtaacgga gctttcatag tagtctgaga accctgaaaa gacagggaaa
      541 gccgtaaaat cggatacggg aaaaccgttc acatggcgaa ggaaagcagt cttagcgaat
      601 taaaattaga aattgattag ggaggaaaac ctcaaatgaa accaacaatg gagattttaa
      661 ccaaattaca ggaaaattca aaaaagaacc atgatgaagt atttacaagg ctctatcgat
      721 atttactccg tccagatata tactatgttg catatcaaca cctctactcc aacaaaggag
      781 caggaacaaa gggtgtaaca gacgacacag cggacggttt tagcgaaata tatattgaaa
      841 atatcattga agccttaaaa aatgaaatgt atcaaccaaa accagtaaga cgtacataca
      901 tcaaaaaatc aaatggcaaa atgcgtccct taggactgcc tgtctttact gataagctga
      961 tacaggaagc aatacgcatg atattagaag caatctatga gcctattttt tcagactact
     1021 ctcacggttt ccgaccagca agaagttgtc atactgctct tgctcaaata aaaaaagaat
     1081 ttacaggagc acgctggttt atagagggcg acataaaagg gtgctttgat aacatcaatc
     1141 atgcggtact tgtggaaatt atcaatcaga aaattaaaga tgcacgtttt ctgaaactca
     1201 ttcgttcatt cttaaaggca ggttatatgg aagattggaa ataccacaaa acatatagcg
     1261 gttgccctca aggtggcatc atttcgccta ttcttgcgaa tatctacctt aatgaattag
     1321 accgtcacgt aatgaagata aagaaagagt ttgatgtggc aacaaaggca cgttatacac
     1381 cagaatatac aaaactggta ggactacgcc aaagattaca caacaaaatt aaaaatagca
     1441 acggtataga aagagaaaag ctgattgaag aatataaatc tgcaacggct caaatgctta
     1501 aactaccagc gaagcaatgt gacgacaaga aaataaaata tgtgcgttac gctgatgatt
     1561 tcctaatagc tgtgaatggc aatcgacagg attgtgagaa gattaagcaa gaacttacag
     1621 aatttataag taccacatta aaaatggaac ttagtcaaga aaaaactctg attacacaca
     1681 gtaatactcc tgctcgtttt ctcggatatg atgtcagagt acgccgtgac cagcagatta
     1741 aaccaaaagg aaaattcaaa acccgttcta tgaataacaa ggtggaattg agtattccat
     1801 tcaaagacaa gatagagaaa tttttatttt caaatggaat tgtaaagcag aggtcagaca
     1861 atgggaaatt agaacctata caccgaccac aactgcttaa taggacagat ttggaaatag
     1921 tgactattta taatgcagaa ttaagaggta tctgtaatta ttatgggttg gcaagcaatt
     1981 ttaacaagct aatctatttc aattatctta tggaatatag ctgtttgaaa acacttgcag
     2041 gaaagcaccg ttccaaagtt tctaaaatta gagctatgta taaagacggt acaggcaaat
     2101 gggctattcc ttatgaaact aaaacagtaa taaagaaaat gtattttgcg aattatgctg
     2161 actgcaaagg aaagaagttt actgatattg ttccacaaac agctaaaaat tattcccatg
     2221 acgtaacaac attagaaagc cgattaaagg cgaagatatg tgaagtatgt gggtgtacgg
     2281 aaagtgacag atacgaaatt catcatgtaa acaaggttaa aaaccttaaa ggaaaatcag
     2341 aatgggaaaa gataatgata gccaaaagac ggaaaacaat cgtggtatgc cacaagtgtc
     2401 acatggctat acatcatggc gaaaagaaat aacctgtaac ggagcaaaga tggagagccg
     2461 tgtgcattga gaaatgcaag cacggttcgg ggagaggact gcacaaacct accctagaaa
     2521 taaggaaagg cggtgctttc ctactctact atgtgggaaa
//
LOCUS       DAEMGG010000235         2505 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEMGG010000235 DAEMGG010000000
VERSION     DAEMGG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.238"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..900
                     /locus_tag="KPK24_003742"
     CDS             <1..900
                     /locus_tag="KPK24_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301368.1"
                     /translation="LVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFKENSWKTL
                     FNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNGEQQNINE
                     IKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDNLNKEKNA
                     NPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYEILDLKVD
                     YNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQVFDMKLKK
                     NIYEAELINGDISLQEKLFFVAN"
     gene            1266..2447
                     /locus_tag="KPK24_003743"
     CDS             1266..2447
                     /locus_tag="KPK24_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2301369.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
BASE COUNT         1030 a          216 c          449 g          810 t
ORIGIN      
        1 ttagtacaag gaatgaatgc tttaaatgat tgtataaagg tttcaaataa aaatttaaaa
       61 agtggggtag aagaaaattt tgatattaat ttaaataagt ctgattttaa agaaaatagt
      121 tggaaaacct tatttaacaa aatatataaa ggtaatttat atatggtaat tagtgaaact
      181 aatatttcag gttctaaagt caataatatt tgtatactaa agatagatat aaaaaataag
      241 tcttataaat tattaaatag atttaattta ggattacaga atggagaaca acaaaatatc
      301 aatgaaatta aattttctat tggaagtaaa ttatacttag aaatagtaaa taataaaagt
      361 aaaaatggat atattgaagg acatgatagt tactctaact ttattatata tgatatagaa
      421 gataacactt ttaatataag taataaattt atggataatc taaacaaaga aaagaatgca
      481 aatccaaagg agattggctt attaagtaaa actaagctag attatatggt aaaaaataat
      541 aaattgaatc ttatagttac ggatatgagt aattccataa ctaatcttgt ttatgatatt
      601 aattctaatg agattaaatt agaagattat gagatacttg atttaaaagt agattataat
      661 gaagcatcat ctggagttag gaaagttaag ctactggatg ataagatata ttctatttca
      721 ggtattaaga acagtatatc aggagaatta aaaggattta aagggattgt tgatgtagtc
      781 ggaactaagc cagtaaaatt tcaagtattt gatatgaaac ttaaaaagaa tatttatgaa
      841 gctgagctaa taaatggaga tataagttta caagaaaagt tattttttgt tgcaaattaa
      901 gtgttagaat taattttaga actttaaact tttatatgta tcacagataa gtataagtaa
      961 gggagtgtct caaaataaac tttttagctc atgagacact ttttttatat gaaatcaaat
     1021 atattttcat tgtttagatt tattttgata atgaaaaaaa gattatcttt attttaagaa
     1081 agacatttat ttataattat atacaaaaat attttttaat gtttttgtgt ataattataa
     1141 caatttaatt tatattaatg cacttaaatt tgaaatagaa tgcttatagt ggtaaaatta
     1201 attattagaa tattagaaat aataaataaa taatacattg tacttaaaag ggggagaaat
     1261 ttaagatgaa caaaaaatat attaaagcag ggaatttaat tgatggaacc ggaaatagta
     1321 ttatgcaaaa taagggtata attattgatg gagatactat agttgaaatt gaagatataa
     1381 gagatgattt aaatggatat gatgtgtacg attacttaga taaaactgtt atgccaggca
     1441 ttatgaattg tcatgttcac ttgacaatgg agccagttgg aaatccaaag atatattatg
     1501 ataatgtatc agatgtagag cttgtagtaa atacgataaa acaacttgat gcttatttgg
     1561 attcaggagt aacatatatt agaagcttag gttgtcccaa atatgtggat gtacaactta
     1621 aaaatcttat agacaaaggg tctatagatg gtcctggtat agttacttca ggtccagtta
     1681 tatgtatgac aggagggcat ggatattatt ttggtattga aagtgatgga gtagatgaat
     1741 gtagaaaatc agccagaaca gttttaaaaa atggagtaga ttgtgtaaag ataatggcaa
     1801 ctggaggtgt aacaactgat ggagttgagc cagggtcacc acaattaact ttggaggaaa
     1861 tgaaggctgc tgtagatgaa gctataaaag caggtaaaat aactgccaca catgcacaag
     1921 gaagaactgg tataaaaaat gcagtgcttg caggaattac ttctatagaa catggtgttt
     1981 atttagatga tgaaataatt gatttgatgc ttgaaagagg aacttactta gttccaactg
     2041 tggctgcacc atattttatt ctaaatgcag gaaaagaaag tggaataact gaagctactc
     2101 ttagaaagtg tgagattgtt gccattccac acaaagagtc attcctaaaa gcatataaaa
     2161 aaggaattaa aattgcagtt ggttctgatg caggaacttc ttataatgaa catggaaaaa
     2221 catattatga aatgaaacta atgtcagatt atggtatgga taatatggat ataatagttg
     2281 ctgctacaaa aacagcatct gaacttttaa gaattgataa aaattatgga actctagaaa
     2341 aaggaaagaa agcagatgtt atagttgtaa atggaaatcc agtagagaat atagacgtac
     2401 ttgcagatgt gttagcagta tttaaacttg gtaaaaaagt tagataaata gtataaaaat
     2461 aaaaaagagt gtctaaagaa atttttaaat aattatagac actct
//
LOCUS       DAEMGG010000236         2490 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEMGG010000236 DAEMGG010000000
VERSION     DAEMGG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.239"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..530
                     /locus_tag="KPK24_003744"
     CDS             27..530
                     /locus_tag="KPK24_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG2301370.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            577..1536
                     /gene="moaA"
                     /locus_tag="KPK24_003745"
     CDS             577..1536
                     /gene="moaA"
                     /locus_tag="KPK24_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG2301371.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            1540..2010
                     /gene="moaC"
                     /locus_tag="KPK24_003746"
     CDS             1540..2010
                     /gene="moaC"
                     /locus_tag="KPK24_003746"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG2301372.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            2046..2477
                     /locus_tag="KPK24_003747"
     CDS             2046..2477
                     /locus_tag="KPK24_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG2301373.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         1030 a          242 c          497 g          721 t
ORIGIN      
        1 atcaatgtat agaggggtga gaacatatgt ttaatgttgc aattataaca cttagtgata
       61 aaggatatga aggaaaaaga gaagatataa caggaaaaaa gttaactgaa tttgttgaaa
      121 atacaggagc ttataaagtt actgaatatg tacttataaa agatgataag gaaatgctaa
      181 aggaaaatat tataagactt tgtaatagta ataaaataga tttaattctt acaaatggag
      241 gtactggttt ttcaaaaaga gatataaccc cagaagcaac taaggaagtg ttggaaaaag
      301 aaataccagg tctgagcgaa tatatgagaa tgaaatccac agaaattact aaaaaggcca
      361 tactaagtag gggtgtaagt ggaataagaa ataattctat aataataaat ttacctggaa
      421 gtcctaaagg tgcagttgaa aatctaagtt ttataattga tgttttagac catggcatag
      481 aagttttaag aggagaagct acagaatgtg ccactaaaaa aagtgaatag tatataatct
      541 agatagataa aaaatagaca aagaggtaga taaagtatga ttgataagta tggaagaaaa
      601 atagactacc ttcgtatttc acttacagat aagtgtaatt taaggtgtgt ttattgtatg
      661 ccaccagatg taaaatttga taaaaattat ataaatgaaa atctaagctt tgaagactac
      721 aagtttatta taaaggctat ggctgaacaa ggtataacaa aaattagatt tactggagga
      781 gaacctcttt tatatcctaa attaaatgag cttataaaat ttacaaaaga agaatgtgga
      841 ataaataaca taggaatgac cactaatgct attgggcttt ctgatagaat ctttgagctt
      901 gaaaaatgtg gcttaaataa agttaatata agtttagatt ctttgaagga atataagtat
      961 aaatccgtaa caagaggagg aaacttaaaa gatgtcttaa aatcgattga atcttgttta
     1021 aacttaggta taaaagtaaa gttaaattgc gttgctataa gtgggtttaa tgatgatgag
     1081 cttaaagatt ttatgttttt aacagttaaa tcaccaattg atgtaagatt tatagagctt
     1141 atgccattag gtgaggctaa tagagtttat aagaagggtt attttaatgt aaaagaaatg
     1201 attgaaaaaa tatatggttt atataaagtc aaaaatgatg aaaaaagtac agcagaatat
     1261 tatatgttta aaggagctaa aggaagaata ggaattataa ctccattaag ctgttcattt
     1321 tgtaacactt gtaatcgtat tagacttact tctagtggtg caataaaatt atgtctacat
     1381 tcaaaagaag aaatagatat aaaaccattt ttacataaac cattgttatt tagagaatct
     1441 atgaaagata taataaaaca aaaaccagaa aaacatcatt taatagaaaa taaatgtagt
     1501 gatacaaata ggcgtatgta tgaaattgga ggataagtaa tgttaacaca tataaatgaa
     1561 aaagggtatg ccaagatggt ggacataagt gaaaaagatg atactaaaag aacggccgta
     1621 gctacagcta caattactct tagtaaggaa gcattagaaa aagtaaaaaa tggtcagata
     1681 aaaaaaggag acgtattgtc tgtagctcaa gttgcaggta taatgggagc taaaaataca
     1741 tcttcaaaca taccaatgtg ccatcagatt aaattagttg gatgtgattt agaatttgat
     1801 gtagatgatt ctatgtctgc tattaagata tacgcaacct gtaaatctct tgggaaaact
     1861 ggagtagaaa tggaagcttt aaatgcatgt tctttagcag cacttacaat ttatgatatg
     1921 tgtaaagcag ttgataaaaa aatggttata agtaatatcc atcttgtaga gaagactggt
     1981 ggaaaatcag gagattttaa gttcgagtaa attaataata tattgggtta aaatttggag
     2041 gaaatatggc gaaggtagtg tcaataaata taagtgagaa aaaaggtgta attaaagttc
     2101 ctgtacaatg tgcagagcta aaaataaatc atgggatagt tggagatgca catgcaggaa
     2161 actggcatcg tcagataagc ttacttggaa aagaaagcat agataaaatg aaaatgaaag
     2221 gctttgacca attaaaatat ggagattttg ctgaaaatgt aactacagaa gggatagaag
     2281 ttttttccct gcctgttggt acaaagttaa gaatagggca atgtgaagtt gaagtaactc
     2341 aaatagggaa aaaatgtcac aatggatgcg aaattaagaa attgacaggt gactgtgtga
     2401 tgccacgaga aggaattttt gtaaaggttc taaaagaagg aattataaat gtgaatgatt
     2461 caatagatgt aatctaatga agtcactatt
//
LOCUS       DAEMGG010000237         2460 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEMGG010000237 DAEMGG010000000
VERSION     DAEMGG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.240"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..424
                     /locus_tag="KPK24_003748"
     CDS             <1..424
                     /locus_tag="KPK24_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2301374.1"
                     /translation="ALDLIWNVFEEFESPDYCDEGITTFHQFIEYNSIIEKIKEKTLF
                     FWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLMTTGIQYCICNKFNLTEI
                     TVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(793..1350)
                     /locus_tag="KPK24_003749"
     CDS             complement(793..1350)
                     /locus_tag="KPK24_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2301375.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSFL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            1608..2306
                     /locus_tag="KPK24_003750"
     CDS             1608..2306
                     /locus_tag="KPK24_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2301376.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNIPTKILYGKHDELTEIGVVEKFVEKFNCNLTIVENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          911 a          321 c          294 g          934 t
ORIGIN      
        1 tgcattagac ttaatctgga acgtatttga agaatttgaa tctccagatt attgtgatga
       61 aggaattacg acatttcatc aatttataga atataactct attattgaaa aaatcaaaga
      121 aaaaactctc ttcttttggg gttgttttga ctctaaaaaa cttattggag tcttagctat
      181 tagaaattta aatcatatat gtatgttatt tgtagataaa aactatcata aacatggtgt
      241 tgcaaaaaaa ttaatgacta caggcataca atactgtatt tgtaataaat ttaatttaac
      301 tgaaataact gtaaattcat caccttatgc tgttgaagtt tatcataaat taggatttaa
      361 agatactgct gttgagcaaa ttgttgacgg tatacgtttt acacctatga aattgttaat
      421 ataataaatt aaataaaaag caaagtctct taaatatttt atagttactt tgcttttaaa
      481 ccaagattta aaataatact atcttttttg ttttttaata atatacttta aaatccttac
      541 taatatgaac tataatttat tttttgatat cctttttgat taacatataa attaattcaa
      601 ttattaaaca taaactatgt tgtttctctt tatatctctt ctttgtaaac aatattgggc
      661 atttgaaaat aaatattctt aaaatatact gttctcctgt tgattttcct ttggtacaaa
      721 tgataaaaat ttaatttaca tgccccataa tgaagtgttc aataaataag ttaaaataat
      781 ttatttacct ccttaatcat attttttatg aatacctaat aacacagtat caatagaatc
      841 gactaaacct tttatagctt cttctttact caaaaatgat ttcccaagtt gcaaagtcga
      901 ataaccatga agtacactca ttattaagtt taatacttca cctgtattct ttgtatttag
      961 attacaagaa agaataagtt tttctaataa ggatttatat tcatcaaaga tttctaatgt
     1021 ttcattattt ccatgccaag tagcccactg aattgtttca taaacaccag gattagaaat
     1081 tataaaatta aagtatgcta tacttataca ctttatagct gtatctccaa aatttccaat
     1141 agccgccttt atcatttgac tattcatctc tctcattcct ttatgagcta cttcaagtaa
     1201 taaatcatct agactttcta tatgattgta caaagatggt gtacgtattc ctagcttttc
     1261 tgcaataact tttaaagaaa ctttgttaag cccatcttta ttaactatat ctgatgtagc
     1321 ttgtataact atttctctac taacacgcat gattttccct ctttcattta aaattaatta
     1381 ctttattagt ttaactaatc taattagttt cgtcaattat tgtaatttat aaaattcttt
     1441 atactattat aaaatcttga cagtttaatt ataatttaat ataatactaa ttaaattagt
     1501 tttaatacta atataattag tattatatta aattctatat tacaacatta ttttatagtt
     1561 tgattaattt aataatcact aatatatcaa actgaaagga gatttttatg gaaaaaaaat
     1621 atttcgaaat tcatggagtt ccttcaatcc tttggggaaa tccttcaagt aaattatata
     1681 tttacataca tggacaatgt ggcaataaag aggaggctga agcttttgct aatattgcag
     1741 ttccacatga gtgtcaagtt ttaagtgttg atttgcctga acatggagaa cgtaaaagag
     1801 aaaaaaattc atttaaccca tggcatgttg ttccagaatt agtatctatt atggaatatg
     1861 caaagtgcca ctggaatcaa atttcgttat atgcaaatag cattggagca tggtttagta
     1921 tgttaagctt tgaaaaagag aacttaagag agtgtatttt tgtatctcca attctagata
     1981 tgcaacagct catttcaaat atgatgctct gggcaaatgt atcagaagaa cagcttaaac
     2041 atgaactaat tatcccaact tcatttgggc atactctttc atgggaatat ctacaatatg
     2101 taaaacaaca tcctatcact caatggaata taccaacaaa aatattatat ggtaaacatg
     2161 atgaacttac tgaaatcggt gttgtagaga aatttgtaga aaagtttaat tgcaacctta
     2221 ctattgtaga aaatggcaaa cattggttta acacaccaca agaattaaat tcattatatg
     2281 aatggttaaa tactttagtt aaataaaaaa taattatatt attaatatga ttaaattatt
     2341 tataaatgtt atttaattaa ttttatttta gcattattaa aataactaca taaagaataa
     2401 attagttatt attaaagaaa ttaaaagtat atagagtatt aataattttt taataattag
//
LOCUS       DAEMGG010000238         2362 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEMGG010000238 DAEMGG010000000
VERSION     DAEMGG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.241"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            22..306
                     /locus_tag="KPK24_003751"
     CDS             22..306
                     /locus_tag="KPK24_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301377.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(463..636)
                     /locus_tag="KPK24_003752"
     CDS             complement(463..636)
                     /locus_tag="KPK24_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HBG2301378.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1077..1292)
                     /locus_tag="KPK24_003753"
     CDS             complement(1077..1292)
                     /locus_tag="KPK24_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2301379.1"
                     /translation="MAIVVNLDVMMAKRKISLKELAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1303..1770)
                     /locus_tag="KPK24_003754"
     CDS             complement(1303..1770)
                     /locus_tag="KPK24_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG2301380.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          951 a          243 c          281 g          887 t
ORIGIN      
        1 gaatgataag gggtggatga aatgcttgta tataataaaa gtttttatcc taatgacata
       61 tttccaagat tagatttttc aaaaataaaa aaacagttaa aattgataga taatgacctg
      121 tcagattttg gaagcatatg tataatagaa aaagaacatt atacgataag tgtaaacagt
      181 ataggtgaaa taaatgtgta ctatgattta gagtacgaaa ataaggtgta tagaatagtt
      241 tatgagattg aaaagttatt taaatctcaa gttggaaggt ttagcatatc tacatacaga
      301 aattgataat taaaaaagta gaaattaaaa aacttaatac taaagatata gataaatata
      361 tgaggtaaca gctagagaga gttgttaccc tttttaaata ctaagtatat attattctta
      421 ttaatttttt gtaaattcac taaaagacct ttttatagaa tattattttc tttttttatt
      481 aaataggtaa atcagtaaaa atgcaattcc agttccaatt attattccaa tgatattttc
      541 tataattaat ttttttaata taaaagtaaa taataaaaat gctatacata aacctaaaat
      601 atatccttta aaataggttg aaagttttct tttcatataa tcaccaactt gtatcagatt
      661 tagtttactt aattagagta taacagtgtt gtaacttcaa agagtatcta aaagattaca
      721 aatgttagat aaaatcatca ataatgtaat ttataacaca tagctataag ctatgcaata
      781 atataaatgg aggtataagt aaatgaaaaa gttccatgtt ttgctaacag tttaaataaa
      841 tattatgtag aaaagaatgt atataggaat ctgtggtaaa attatcaatt taaaagtatt
      901 atttagtgtg atacagttct tataggtaat ggataacaaa tagataaagg aacagtttct
      961 ttcttagaac atattttgtt atgactttaa acaaagttaa tattaatgtc aataatatag
     1021 taaaagaata aaaatcatat agtataataa aaaagagtgt ttctaaacac tcttttttaa
     1081 tcattttcat cttctacata ttcaagtata tcacctggct gacagtctaa agctttgcaa
     1141 atttcgttta gagtggtaaa tctaatagct tttgcttttc cagtttttaa aattgataaa
     1201 tttgcatttg taatatctat tttttctgcc aattctttaa gtgatatttt tctttttgcc
     1261 atcattacat ctaggttaac tactattgcc ataacatcac ctctatatcg ttaaatcatt
     1321 atcttctttt atttttatag ctttatcaaa tgtttctgca attacaaagc acattagtgc
     1381 aaatataaaa gagataacca taagtggtga gataccgcca ctatcagttc caaaaattct
     1441 tatattatta gtatttaaat tataaccatt gatacattca attatagtgt ttatcaataa
     1501 acaaaatccc attactttaa acctttttac attttcaagt acgaatgggg aatacttagt
     1561 actattaatt ataaatatca agaataagat gactataaag tagtcagaaa aatatagtat
     1621 tcttgcaatt aaagcaatta ctttgtcaga actaaaatca tataaaaaca taggtatcat
     1681 acataagaat attataccag caactcctaa aagtgataat agaaaatata gaaatactga
     1741 taatatatta ctaaaaaatt ttgtaatcat aaaatatcat ctcctttata taaagtatat
     1801 tattgattat aatatataat ttattgaatt tcaataataa aatattgata taatatataa
     1861 aaccattgta atatacacta aaaataagaa tgtataaggt ggtaaattta tataaaaaag
     1921 gatatgagtt gtaaaatatt aaataatatt gtactaattg gaataatttt aactttatta
     1981 tagattattt taatactatt agtactgatg gtgtatttta aaattggtat aagcattaca
     2041 aatacgaata tgccaactgt aatataagta gctatataca tatgtgctgt cccatatatt
     2101 gtatcattgt ttatattaaa aagattatgt aaattggtat caatcaataa atcattttca
     2161 gaagaaatac ctaaaaactt aaaaaatacc tatatgtgta tttagttaga tttgagattt
     2221 ttacatttaa ttttatagtg acaatattat attattttta tgatgtctat ttatatgcaa
     2281 ttactataat atcagaactt atagtatcat ttgtttcatt atcaataagg ttttttactt
     2341 tagttttgtc aattttatta aa
//
LOCUS       DAEMGG010000239         2300 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEMGG010000239 DAEMGG010000000
VERSION     DAEMGG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.242"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1126
                     /locus_tag="KPK24_003755"
     CDS             <1..1126
                     /locus_tag="KPK24_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HBG2301381.1"
                     /translation="IIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCIAFEEYFRL
                     ILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILLISYIESVA
                     FSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEIDVQETKDG
                     ELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVGEKIPTLKE
                     MIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKTILQKVKKL
                     DENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFVWTVNSDSL
                     IEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            1448..2032
                     /locus_tag="KPK24_003756"
     CDS             1448..2032
                     /locus_tag="KPK24_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG2301382.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSLDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT          889 a          221 c          378 g          812 t
ORIGIN      
        1 tattattgga tatgcgattt atcatagtgg aattatattt ataggcatat ggactaaata
       61 ttgtactgat atggaatttt tgaagaatgt atttataaat agatgtattg catttgaaga
      121 atattttaga ttgattttat caatattcat ttttatattg gacattggat ttatctcagc
      181 aacttattat caatataaag gagtaaatat ttctaaaaat aatattaaaa aagagaaaaa
      241 atctatatca aaaattgttt ttaatttagt attaatatta ttgatttctt atatagagtc
      301 agttgcattt tcttcacata taaatggtat gtttaatacg tcattttcat tttatacaac
      361 agcaattgct catagagcag gagctgaaat ggctccagag aatactttgg cagcaataga
      421 agaagcttca catgctaaag ctgattatgc agaaatagat gttcaagaaa ctaaagatgg
      481 agaacttatt gtacttcatg attcaaactt taagagaact acaggagtag ataaaaatgt
      541 ttgggatgta aattataatg aagtaaagac atatgatgcg ggaagctttt attattatgg
      601 aaaaggttat tatgatgaaa agtttgttgg ggaaaagata ccaacactca aagaaatgat
      661 aaaatattct aaaggtaagg tgaaattatt aatagaaata aaacttaatg gacatgaaaa
      721 gggagacgta gtaaaaaaag ttgttcagtt aattaaagaa aataattttg aaaatcaatg
      781 tatagttgca tctatggata aaactatatt acaaaaagta aaaaaactgg atgagaattt
      841 actgacatgc tattttatgg caattgcata tggtgatttt tcaaagttaa attatgttga
      901 tatatatgga attgaatcaa cttttgttaa taaaacagta gtagaaaaga ttcataaaat
      961 gaacaaaaaa atcttcgttt ggactgttaa tagtgacagt ttaattgaga aaatgcttga
     1021 cctaaatgta gatagtatta ttacagataa tccatacctt atagagagtg caatttattg
     1081 gaagaaaaat ggatttatta gtcaagtgtc agattattta ttttaaatat tctataattg
     1141 tattatccag taaaagagat attattaaaa tcaataatat ctctttttaa cataaataag
     1201 gttgttaaat taaggctatt cttataaaaa ttgtgtttat cagatataaa taaggaataa
     1261 aagtaaaaaa attgctatga cattattaca attaaaagag ttttaatatg atagagaggt
     1321 agattgtcaa ctgtatattt aataaatttt attatttatt agattttata taagtaaaat
     1381 atatagatat ataaatctta tgctagaata tatatgaaaa acaatataga tttggagggt
     1441 taaaagcatg acagagcgag aaaaaatgtt ggcaggagaa ctttacgatt gtggtgatat
     1501 tgagttgtta actcaatggc ataaagcaaa agacctagta agagattata accaaacaga
     1561 ttccttggat ttggaaaaaa aagaaaaaat attaaatgag ttacttggtg gtaagggtaa
     1621 aaacttatgg attactgcac cgtattatgt agattatgga aataatattt attttggaaa
     1681 taattgtgaa gtcaatatga attgtacgtt tttagatgat aataaaatta tcattgggga
     1741 taatgcacta atcgcaccaa acgtacaaat atatactgca tttcatccaa ctaatgctgg
     1801 tgatagattt ggtaatgcca aagaagatgg ttcatttgaa ttttgtaaaa ctcaaacagc
     1861 tccagttact attggagata atgtttggat tggcgggggt gctattatca tgccaggtgt
     1921 tactattgga gataatgtag ttataggtgc aggaagtatt gttacaaaag atattccaag
     1981 taatatgatt gcatacggaa atccatgccg tattattcga gaaaataaat aaagttaaat
     2041 tttcttgcaa aatctcaaaa aacacctttc ttactttata catagggtat tttctaatac
     2101 tttcacatcc atattcattt gttaaatttt tgtggaatgt agtttttatc acttctatct
     2161 tacatttcca ccattttttt ataaaagtca ttcctttata gatttattta taatagtata
     2221 tattctattt gatgtttgaa taagcactta tttttaagta aaatactaac ttctaggtta
     2281 gtattttact atagtatgat
//
LOCUS       DAEMGG010000240         2251 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEMGG010000240 DAEMGG010000000
VERSION     DAEMGG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.243"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(144..1711)
                     /locus_tag="KPK24_003757"
                     /pseudo
     CDS             complement(144..1711)
                     /locus_tag="KPK24_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(1686..1829)
                     /locus_tag="KPK24_003758"
     CDS             complement(1686..1829)
                     /locus_tag="KPK24_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301383.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(2047..2229)
                     /locus_tag="KPK24_003759"
     CDS             complement(2047..2229)
                     /locus_tag="KPK24_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301384.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
BASE COUNT          704 a          310 c          253 g          984 t
ORIGIN      
        1 tttttaatac aatatagttc taaaaaagaa tatattagaa gaagataata tttaaataaa
       61 aaatacctta taaagttaat tttttaatac tttataaggt aaatacatat tgtattgtgg
      121 ggataaattt aaatcatttg atactaatca acactaagaa ccaataactc tctatcatct
      181 tgagtaattg tatatattct taatttcttg tatgtcataa tattttctcc acctgtaaaa
      241 taactcctaa gattaacaca atcagtttta ggttgatttg tatctcctaa ataattacgc
      301 aattctttat ctttagtttc aatatcttta aatccaaaat attgtaaagt agcttcaact
      361 ctatattttt ggacataatc gagagcttct ttattagttt atgttggtgt aaaatacata
      421 ccatttatat aagcctttat tgtacttgga ttaaatttaa aatctgcaga atatatttta
      481 tgtgtaacta ttatttcttg aacagttgga attttaactt ctggttcatc aagtatttca
      541 ggagtactat ttagctctgt aatagataag ttatctaagt atattgtatc actatcaatt
      601 aatgctatat ttatattaga agaatctttt tcagtagttt caaattcata actaaatttt
      661 gtatatcctt gttctagtac caaataatcc gttttttctt cttttacttt tatatttaca
      721 attattgaat tatatgttaa aggatttttt gaattttact aaaaacataa cgtttataag
      781 gttttagttc agacataggt attttaatct tcgtctcacc atttaatcta tttgctgagc
      841 cttttaaacc atcttgattt gtagtattaa cattactcca tatactaggg taattattgt
      901 aatcatcaaa attagtaaag taagttggtg tttttataag tatattcatt cctctttcaa
      961 gtttaacatt acatatttgt ttatcagaca aagtttttaa actgtcttta atcttattgc
     1021 ctatattatc atcaaatatg agttcaacac aactttcatc tattggtata ttattaaaat
     1081 ataacaaacc atctttttta gtagtgccaa aagttttttc aattgcttct ccaattgtaa
     1141 gttcaggtgt tttatcttct gggacctgta aacttttagc agtaattctt cttttgtaag
     1201 attcattttc tgtatctaat ataatagata cagaaatact gtcaatttga cctatatcgt
     1261 cttaccaact atttcattct gttactattt gtccagaacc attttttgta ccaaaatttc
     1321 cacttacttg tgttgtttct aatttaattt gctttccatc atctaatttt tttaattaat
     1381 tataatttat aggaatcagt ctagaactaa attgatacat tgtgttaaga gccataggtg
     1441 aaaatccttt tttgagataa gtatttccag gagatagatt attgccaatt tgattttctt
     1501 gtgctttgat agttgataat gcatctccat ctaacactaa atttgttgtt ggtgtcactt
     1561 tgtacatagg tgcagtacct gtgttgtagt atctaatatt tgcatttata tatgcagatt
     1621 ctcctttgtt tatacttaat ccagtatttc atgattatcc attgctattt tgaacagcag
     1681 ttgaattatc tgttgtatgg taataatttg tcttgtatag tttctttaaa ctcattaaat
     1741 ttttctaatg aaatttcgtt ttgatttttt gttcttatta ctttattaaa tgcaaatttt
     1801 tctggagatt caaaataata tacatacata ggtttatcta ttttattttt tgatatagca
     1861 tttcgaagtt ctctatattc tttttctcaa gaatttgctt ctatttgata atcataaaaa
     1921 taatttcttg tctgagaata tttgcttatc tctatagaat cttttttata tgatcctaat
     1981 acttcttttt cagatttttc aagtttttga tcttttcttt ctcattcttt agccttttct
     2041 ttatctttaa aaaaatcctt ctgatctttc tataggagcc ttgtaagtag tattacaaac
     2101 ttttccacta tagctttgta aatcttgtgc ataaatgtta ttaggtaaca aactacctaa
     2161 agttagatat aatatcaaca atatagatat acaattacta atacttttat gtttcctaaa
     2221 ttttttcatt tattctccct acaaatatta g
//
LOCUS       DAEMGG010000241         2238 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEMGG010000241 DAEMGG010000000
VERSION     DAEMGG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.244"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1584)
                     /locus_tag="KPK24_003760"
     CDS             complement(<1..1584)
                     /locus_tag="KPK24_003760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301385.1"
                     /translation="MLTTNIHYILDNDICEIYFNKQRLTNSKYYQDNINVLNYIINEK
                     NNNLIINYNDKYIYAIYLLNIAINSYLNNTLNPNNHILTKLDKGMPLLYKGQIVIFKN
                     INNTHITLGHKNKTDTTLIPIDSAYMLITYNGHLEVNSMGKTPASKTFQTKNVLSSLG
                     FSDLKNMTGVINDSTLIILPCKDDISDLVSNIKIKDINNTYKFTELFPCSYISSTGAE
                     TDYPGNHAKQIPLLKFTTNISSAFEIIKSDKSIKNVFIIGDYPIYKHINDFERILNRK
                     RIESVNIVTSNSNISNILDLPNIDNLNIYSWSKDVLLTYSQDFENTSTFQKLWLDKLI
                     NKNICTTSVDSNISDLIHQARKSLYYISKYDFDISIKNSLMMCSYSLLKILENTPFNL
                     DFLEETIKLLNLNIATPSAKLENIKYLISSTSPDLELSALFDDVIVKFERIINELSDN
                     NTKFNTLNKLVSSYKYKTFTPNTPVILLQKQYEAIILKNLLKSRNINLQVISAEKFKD
                     SKTIKNLIMFGFYDLKYLDI"
     gene            1896..>2238
                     /locus_tag="KPK24_003761"
     CDS             1896..>2238
                     /locus_tag="KPK24_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG2301386.1"
                     /translation="MYNVIDLFAGAGGLSLGFEMTKKFNMVAFVEKNDNAAKTYLENH
                     PSVKRYCDIKRLDFQDILNSVDKIDVVIGGPPCQGFSNANRQKRKIINGNNELVKLYV
                     DAIDKLKPNVFV"
BASE COUNT          836 a          210 c          310 g          882 t
ORIGIN      
        1 aatatctaaa tatttcaaat catagaatcc aaacataata agatttttta ttgtttttga
       61 atctttaaat ttttcagcag aaataacttg taaatttata tttctagatt ttaataaatt
      121 tttaagtata atagcttcat attgtttttg tagcaatata acaggtgtat ttggtgtaaa
      181 tgttttgtat ttatatgaag atactaattt atttagtgta ttgaatttgg tattattatc
      241 agataattca tttataattc tttcaaattt gactataaca tcatcaaata atgcacttaa
      301 ttctaaatct gggctagtac ttgaaattaa atattttata ttttctagct tcgctgaagg
      361 agttgctata tttaaattta aaagctttat agtttcttct aaaaaatcta ggttaaaagg
      421 tgtattttct aagattttaa gaagagagta actacacatc ataagtgagt tttttatgga
      481 aatatcaaaa tcatacttag atatgtaata aagtgatttt ctagcctgat gtatcaagtc
      541 agagatatta gaatcaacag aagtagtaca tatattttta tttataagtt tatctagcca
      601 taatttctga aaagtagagg tattttcaaa atcttggcta taggttaaca gaacatcttt
      661 agaccaagag tatatattta gattatctat atttggtaaa tctaatatat tgcttatatt
      721 tgaatttgag gttacaatat ttacactttc tatccttttt ctatttaaaa ttctttcaaa
      781 atcatttata tgcttataaa ttggataatc acctattata aatacatttt ttatagactt
      841 atcagattta ataatttcaa atgcactaga aatattagtt gtaaatttaa gtaaaggtat
      901 ttgcttagcg tgatttccag gataatcagt ttcggcacca gttgatgaaa tatatgaaca
      961 tggaaatagt tcagtaaact tataagtatt attaatatct tttattttaa tattactaac
     1021 taagtcagat atatcatctt tacaaggcaa tataatcagt gtagaatcat ttattactcc
     1081 agtcatattt tttaagtctg aaaaacctaa agatgataga acattctttg tttgaaaagt
     1141 cttagatgct ggagttttgc ccatagaatt gacttctaaa tgaccattat aggttataag
     1201 catataagcc gagtcaatag gtataagtgt tgtatccgtt ttatttttat gtcctaaagt
     1261 tatgtgtgta ttatttatat ttttaaatat aactatttga cccttgtaga gcaggggcat
     1321 acctttatcg agttttgtaa gaatgtgatt atttgggttt aatgtattat ttaagtatga
     1381 gtttatagct atattcaaca aataaatagc atatatgtat ttgtcattat aattgataat
     1441 taagttatta tttttttcat ttataatata atttaataca ttaatattat cttgataata
     1501 cttagaattt gttagccttt gtttattaaa ataaatttca catatatcat tatcaagaat
     1561 ataatggata tttgtagtta acatttaaat ctcctttagt agttactatt gtttattaga
     1621 atagttttta ttctatatta taattatatt actaaaaaat aatatatggt atataatata
     1681 aaaaaagatt gataaataaa taaaaaatat attatgttat ttatttgaat agattatcca
     1741 gaagacataa ataagaaaaa taatgtgtaa gtaaaaatat tagaatttat taaaagctat
     1801 tacaagtata agaaatttga aatgattatt caaaaattga catgtgaaaa aaaatatgat
     1861 atgctgcaat aaaagttaat gtgaggtatg tcagaatgta taatgttata gatctttttg
     1921 caggtgcagg tggtttgagc ttaggatttg aaatgactaa aaaatttaat atggtggctt
     1981 ttgtagaaaa aaatgataac gcagcaaaaa cataccttga aaatcatccg agtgttaagc
     2041 gttattgtga tattaaaaga ttagattttc aagatatatt aaacagtgtc gataagatag
     2101 atgttgttat aggaggaccg ccatgtcaag gtttttctaa tgctaataga caaaaaagaa
     2161 aaattataaa tggaaataat gaattagtta agctatatgt tgatgctatt gataaattaa
     2221 agccaaatgt atttgtaa
//
LOCUS       DAEMGG010000242         2194 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEMGG010000242 DAEMGG010000000
VERSION     DAEMGG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.245"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..327
                     /locus_tag="KPK24_003762"
     CDS             <1..327
                     /locus_tag="KPK24_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2301387.1"
                     /translation="IICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWHGRQ
                     IVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRL
                     ITIQNK"
     gene            460..1965
                     /gene="ade"
                     /locus_tag="KPK24_003763"
     CDS             460..1965
                     /gene="ade"
                     /locus_tag="KPK24_003763"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG2301388.1"
                     /translation="MSPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADPHELVNV
                     KGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNNPRILGL
                     GEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDHECITFD
                     EVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDIEKQGHI
                     RWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLDKMEVDS
                     VYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEIVPYQIL
                     TNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAVAPLKGFGIKNGAIATTVAHD
                     SHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFTAEEVQE
                     ITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT          845 a          238 c          415 g          696 t
ORIGIN      
        1 attatttgca tagaagattt acaagtaaag aatatgatta gaaatcgtaa actatctcgc
       61 ttaatttctg atgtgtcatg gtcagagttt attcgtcaat tagaatacaa ggctaattgg
      121 catggtagac aaattgtaaa ggtaggtaaa ttctttgcaa gttcacaaat atgcaataaa
      181 tgtgggtata aaaacgagga agtgaaggac ttaaatataa gagaatggat ttgtcctagt
      241 tgtaatgaaa ctcacgatag agatataaat gcaagtataa atatattaaa agaaggatta
      301 agactaataa caattcaaaa taaataacgt atgtaagaac cgtaggaact acggggatag
      361 cctgtggaga attagtaaga catattttag tatgcaaaat tctgtgaagc aggaaccctg
      421 tgactttagt catgggaggt tcagtgtagc aataaatata tatcacctgg ctttattgat
      481 gcacatatgc atattgaatc tacaatggta atgccaattg aattatcgaa aaaattatta
      541 aaatcaggta cgacaactat catagccgac ccacatgagt tggtcaatgt aaaaggagtt
      601 agtgcaatag attttttatt agaatcgaca aaagatatac cacttaatgt atatattatg
      661 gtgccttcat ccgtacctgc aacaagtttt gaaactaatg gagttggtaa atttagtgca
      721 aaagacatgg agagttatgt aaataatcct agaattttag gtttaggaga ggtaatgtgt
      781 tttaatgatg ttataaattc tgaaaatgaa atcttggata aactagagct ttttaaaaat
      841 aaagttgtag atggacatgc acctaatata aatggtaaat cacttcagac atatgtatgt
      901 gcaggaatag aaaatgacca tgaatgtata acttttgatg aagtatatga aaaattgaga
      961 gcaggattaa aaattttaat aagagaagga agcgcagcaa aaaatttaaa gtctatagta
     1021 agtggtatgc taaagcataa cttgccaatt gaagaattta tgttttgcac agatgataag
     1081 cacttagatg atatagaaaa acaaggtcac ataagatgga atataaaatg tgcgattgat
     1141 ttaggtatgg aacctgttag ggctataaaa gttgcaactt ataattctgc aaaagcatat
     1201 gggttaagga aaattggagc aataggtgcg ggttataagg cagatattgt tgtattgaat
     1261 gatttagaca aaatggaagt ggatagtgta tataaagatg gtaatttggt aaacgaagag
     1321 atgttttcta attacaatta tgagattaaa gataaagaat tgcttaatac agtcaagttt
     1381 aaatatatta ataaagaaaa aatacagtta aaagtaagtg aaaaaaatta tgtaatggag
     1441 attgtacctt atcaaatttt gacaaataag gtatatgaat ctttaccatg tgcagatgga
     1501 tattttgttc caaataaaga gtattcaaaa ctttgtgttg ttgagagaca tagaatgact
     1561 ggaaatgtag cagttgctcc actaaaagga tttggcataa aaaatggagc aattgctact
     1621 actgtggccc atgattctca taatattata gttgcagggg ataatgatga tgatattcta
     1681 gtagctataa attacttaaa ggaaatacaa ggtggttatg taattgtatc aaatggaaaa
     1741 gttcttgcac acctaagtct tcaagtagct ggattaatta gtacttttac agctgaagaa
     1801 gtacaagaaa ttactgataa tatgttggag atagcaagaa aaatgggagt tccagagtat
     1861 gtagaccctt ttattacact ttcttttatg gcacttccag ttgttccaca aataagactt
     1921 acagatttag gtctatttga tgttgaggaa tttaaattta tatagataat ataaaagtaa
     1981 taataaatga ggctttctca aaatagggat aagtctcttt ttattgttga aatgataaaa
     2041 atatatttga tttcatacaa aaaagagcat ctcatgaact aaaaatttta tttttagata
     2101 tccttatttt aattaatcta tcatttaacc ttgttttaag catagatatc ttaataaaac
     2161 tattttcagt ctattttaac atataaatac atga
//
LOCUS       DAEMGG010000243         2103 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEMGG010000243 DAEMGG010000000
VERSION     DAEMGG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.246"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            278..652
                     /locus_tag="KPK24_003764"
     CDS             278..652
                     /locus_tag="KPK24_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2301389.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            649..1512
                     /locus_tag="KPK24_003765"
     CDS             649..1512
                     /locus_tag="KPK24_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2301390.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1512..>2103
                     /locus_tag="KPK24_003766"
     CDS             1512..>2103
                     /locus_tag="KPK24_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG2301391.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFV"
BASE COUNT          797 a          219 c          314 g          773 t
ORIGIN      
        1 aagttcacct tataagtaaa cttttattta gctataagct aaactttatt atttttttaa
       61 tttagtattt ccaatttatt ggcaggattt ttgaacaata tttataaatt ttttaaaaaa
      121 atttaaatct atttaatatg gcagactttc ataaaataca ttacatttat taaatgtata
      181 atattggcaa ttagtttttt ctaaaatcag ttgacatatt atatatatat ttatataatg
      241 tgtatatact atatatacaa tttaaggagg tgtcttattg aatatagtta ttagtaattc
      301 gagtgggaag ccaatatatg aacagattac tacacaaatt aaaagtatga ttataagtgg
      361 agagttacca gaaggctcag ctcttccaag tatgagacta ttggcaaaag aacttagaat
      421 aagtgtaatt actacaaaac gagcatatag cgatttagaa cgagacggat ttattgagac
      481 agttactggt aaaggcagtt ttgtatctag taaaaacatg gattttgtca aagaagaaca
      541 atttcgttta attgaagaat acctacaaaa agcagttgat acagcaaaaa gtagtgatat
      601 tacatatgac gaattaaaag atattttatt tttattatat aaaggagaat gataagtatg
      661 gatgcaataa ctataaaaaa tttaaataaa acttataaag attttagcct acaagatata
      721 tctttttcag taccaaaagg gagtgtaatg ggatttgtag gtgaaaatgg tgcaggaaaa
      781 acaactacat taaaagcaat tttaaattta atatcttatg atagtggaaa tatagaaatc
      841 tttggtcttg atagtaaaaa aaatgaaaaa gaaattaaag agcaaattgg ggttgtattt
      901 gagggaagca actttcatga aaatttaaac actgaccatg tttcaaaaat catgagtaaa
      961 atctataaaa attggaatga cacattattt aaagactact taaaaaaatt gagagtacca
     1021 gacaataaat taattaagga gttttcaaag gggaataaga tgaaacttag tattgctgta
     1081 gccttatctc ataaaccaaa attattaata ctagatgagg caactagttc acttgaccct
     1141 atcgttcgtg aagaaatact agatatattt ttagatttta tacaagatga agaacactct
     1201 attatcttat cttcacatat tacaagtgat ttagataaga tagcagacta tattacattt
     1261 atacataaag gaaaaattgt atttagtgaa aataaggatg aattaattga tactatggga
     1321 gttttaaaat gtaaacctag tgattttgat aacctttcaa gagaggatta ttcatattat
     1381 cgtaaaagcc aatttggata tgaagtactt cttaaagata agcatagatt tatctcaaga
     1441 catccaaact gtattgttga taatacgtct atagaagaaa ttatgttatt ttatgtgaga
     1501 ggtgataaat aatgaaaggt ctaattttaa aagatttact taatttgaaa ggaaatataa
     1561 agtttatact tttattcatc attatgtttg gctttatgtc ttctttagga gatggaaatg
     1621 ttaataactt tataggtgtt attatagttt tatgtactac aatgattgtt tcaacatttt
     1681 catatgatga tttgaataaa tgggatagtt atgtacttac aatgcctatt aatcgcaatg
     1741 atattgtatt aagtaaatat ttaactatgt taatatttag ctttataggt gttcttgttt
     1801 cacttattgt tagtgtaact attggttatt ttaaaaatac acttatatta aatgaaacac
     1861 tacttattaa tgcactaatc ttatcaattt cagtatgttt tggtagcctt attctaccac
     1921 ttatttataa atttggtaca gaaagagctc gtttattaat gattttatgc tttttagttc
     1981 caacactagc tttattagtt tttaaaagta ttttagaaaa tattagttca cctatttcta
     2041 tagaaattat acttaataca ctagtatatt ctcttccatt tgttgctatt ttactatttg
     2101 tga
//
LOCUS       DAEMGG010000244         2085 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEMGG010000244 DAEMGG010000000
VERSION     DAEMGG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.247"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            471..1799
                     /locus_tag="KPK24_003767"
     CDS             471..1799
                     /locus_tag="KPK24_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2301392.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          743 a          227 c          357 g          758 t
ORIGIN      
        1 aatattagtt ttgatttctg gtttaaattg ggttataata aattgtcaaa ttatttatta
       61 atataaaata gactataaaa caagcaaaaa tcatactatt atataaaaga ccttaaagat
      121 agaaaactac agaaagaagc caaaatgttt ttgaaaaaaa tgaaatttta ttgcaaaaaa
      181 atcaaaatga atttaactta atagttataa tctattttta gtgttacaaa tatacaatgt
      241 atattataca atataaaaaa tacatttact tagaagtatt attatatttg gatttttggt
      301 ttttataatt tttttatatt taaacatagc taaaaataaa agattatgaa agctaagtag
      361 agtttatttt attttaataa ataataaagc tatttttcta tatattggca taatatatgc
      421 ttatttaact atgaaattaa aattaaatag gattaatgag gaggaaaaaa atgggaagta
      481 atgaactgaa aaagactctt ggcgtatcag cagcattatc aactgttgtt ggttccgtta
      541 taggagcggg cgtatttttt aaaccacaag cagtctatac attaactgga ggagctccag
      601 gtcttggtat attggcatgg ttaatagctg gaatcataac tataacagct ggtcttacag
      661 ctgcagaggt atcagttgcg ataccaaaaa ctggtggaat gatggtatat ataaaagaaa
      721 tttatggaga aaaattaggt tttttaactg gatggatgca aattgtatta ttttatccag
      781 ggatgatggc agcattaggt gttatatttg gagaacaagc atcagcacta attggtagtc
      841 catcactttt acttccaata gcaattggaa ttatagtaat agttgcaggg cttaatatgt
      901 taggttcaaa aactggagga gtcatacaaa ctgtttctac aatttgtaag ttaataccac
      961 ttattttaat aatggttgtt ggatttatta aaggtggtgg aaataatcca atacttacac
     1021 ctatggttgg tgaaggatta agcctgggta gtgtactagg tcaagtttta attgctatat
     1081 tatttgcttt tgatggatgg atgaatgttg gtactttagc tggagaaatg aagaatcctg
     1141 gaaaagacct tccaaaagct ataataggtg gattgtcagt tgttatggct gtttatttta
     1201 taataaattt agcgtatctt tgggtattac cagctaatga attagcaaat tatgcttctc
     1261 ctgcatcagc agttgctgaa gttatatttg gttcaatggg tggaaaaata atatctgtag
     1321 gtatattaat ttcagtgttt ggagcattaa atggattttt actaactggt tcaagggtgg
     1381 catatacttt agctacagat aagactttac caaagtatgg tatattttct aaattaaatt
     1441 ctgctcaagt tccagcaaat gcaatagcat tagtatctgt tattgcatct atatatgctt
     1501 tgtcaggtca atttaattta ttaactgact tagctgtatt tgctacttgg atattctatg
     1561 tattaacttt tataggggtt atgaaactta gaaagactca tccaaatata ccaagagagt
     1621 ataaagttcc attatatcct attgttccta taatagctat agcaagtggt atatttgttg
     1681 tagttaatca attgtgtttt gcaggtatga aaactactat gatatctata ggaggtcttg
     1741 taataacagc tataggctta ccagtgtatg cttatatgac tagaggagta aaaagataaa
     1801 ccaagtagtt tacaaatgct tcataattaa aataaaagag actgtctcaa atagagataa
     1861 gtctcttttt tattgttaaa atggtaaaga tatgtttaat tttacaaaaa aagagcgttc
     1921 catgaactaa aaagtttatt ttaaggtatt cattttattt ttagtttttt gattataaaa
     1981 ttaaacatct aaaaaattat ttattttaaa atactttatt ttaaagtgtt ttttatcaaa
     2041 ctaagtggta tctgtacaac aaatattata catagctttt atgaa
//
LOCUS       DAEMGG010000245         2080 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEMGG010000245 DAEMGG010000000
VERSION     DAEMGG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.248"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(81..1373)
                     /gene="brnQ"
                     /locus_tag="KPK24_003768"
     CDS             complement(81..1373)
                     /gene="brnQ"
                     /locus_tag="KPK24_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG2301393.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGIK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          688 a          351 c          237 g          804 t
ORIGIN      
        1 ttaataattc ttttaaatat aaaaaggaat tgcctatata atttaaaggc aattccataa
       61 ttatcaatta gtttttatta ttatgcagtt ttactgcttt tagatggaat aaagtttcct
      121 attattcctg ctattacaac tggaactatc cagttaaatc ctaatgaagc aaatggtaag
      181 ctattaacta gaggaatttt tataccaaaa cttgttgcaa ctgttaatac acttactaat
      241 aatgccatgt atgcagcaca tttgaacacg ttgtcatttt taattttatt actaaataat
      301 gctagtataa ctagagttat agttgctgga tataccatac ttagtatagg agaagagaat
      361 tgtattattg tacttactcc aaagtttgat atgattgcac taaatacaca aactactata
      421 actatatgct catactttaa ttttccatct gtaagtgttg agaaatattg accagctgct
      481 gaagaaagac ctatagctgt tgttaaacat gctaatgcaa ctataatagc caataaaatt
      541 ttacctgtag ttccaagtaa agctgctgtt attgcaacta ttaaagctgt ttgttgaaca
      601 tcttgtccat attgttgaga cacagttgct ccaagataac ataatccacc ataaactaat
      661 gccattccta ttactgcaat tacacctgca cttattgtgg ctttcatctt ttctttctca
      721 cctgtataac ctttagctat tactgacatg attataattg atgcaaatac actagctgcc
      781 aatgcatcca ttgtttgata accttgagtt ataccttctg caaatacatt ttgtattaaa
      841 gaactgtctc ttatttctcc taatggagat acgatacctt taactattaa tactgctaaa
      901 gctataagta aagctggagt taatattgac ccaactatat ctacaacttt tgatggtctt
      961 attgttaaaa ccaatactag agcaaagaat actatagaga atattactgg gcttattcca
     1021 gaacctattg taggtaatat acccatttca taagttgttg ctgctgttct aggtattgct
     1081 aaaagtggcc ctatacagat taccattgca catcctaatg caatacttaa tcctttgcct
     1141 gctcttgaga acatcttcga tgtgtctcca ttgaacttag ttgctgctat aactgctaat
     1201 aatgccaaac caccatctgc aaaaaggaat cctatgaaag ctactagcca actacttcca
     1261 gataaaactc ctagatatgg agggaagatt aagttccctg caccaaaaaa cattgcaaat
     1321 agtgcaaatc caattacaat tacatcttta aaattgattt gtttctcacc catttaaagc
     1381 acccccattt gaattttact accttttact aaaaaataag tccaatctct taattaaatg
     1441 aaaacctttt gacgagattt tatttaatta tacatttttg acttaaattt gtcaattatg
     1501 tttcataatt tataaaatct tcacaatatt taatacaact ttcttaatta aacaaaatca
     1561 acctgtatac tttaaacata gttggttttt tgttttttct ctaatttttc ttgataaaat
     1621 taactattcc gaataattaa aatattaaga ctttttagat tattcgattt attattaagt
     1681 tgtattgctt tatatactgt atatatcact ctcctttttt acattcatat aatcttttta
     1741 attcaattta aattttaaat attttgtgtt tattatataa tctgaaaaaa aagcagtcaa
     1801 tagcattttt aattttttta attttcaaaa ttatgattct taaaaaatcg ttttcctttt
     1861 atcataacac ctatttacgt taattcttgt agtattttgt atttattttg ctaaattgac
     1921 agttaacttt attcaatttt tattctactt acgtaaattt gcctcttgtt ttttactctt
     1981 aaaaccattt tttctgaaaa ttccttatta tgatttatca acttattttt cttcataaca
     2041 attactctct aacttccaac ttacttaaac tttgcagttg
//
LOCUS       DAEMGG010000246         2020 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEMGG010000246 DAEMGG010000000
VERSION     DAEMGG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.249"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            118..234
                     /gene="rrf"
                     /locus_tag="KPK24_003769"
     rRNA            118..234
                     /gene="rrf"
                     /locus_tag="KPK24_003769"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            241..315
                     /locus_tag="KPK24_003770"
     tRNA            241..315
                     /locus_tag="KPK24_003770"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:273..275,aa:Asn,seq:gtt)
     gene            320..394
                     /locus_tag="KPK24_003771"
     tRNA            320..394
                     /locus_tag="KPK24_003771"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:353..355,aa:Glu,seq:ttc)
     gene            400..475
                     /locus_tag="KPK24_003772"
     tRNA            400..475
                     /locus_tag="KPK24_003772"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:433..435,aa:Val,seq:tac)
     gene            481..557
                     /locus_tag="KPK24_003773"
     tRNA            481..557
                     /locus_tag="KPK24_003773"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:515..517,aa:Asp,seq:gtc)
     gene            568..642
                     /locus_tag="KPK24_003774"
     tRNA            568..642
                     /locus_tag="KPK24_003774"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:600..602,aa:Thr,seq:tgt)
     gene            657..741
                     /locus_tag="KPK24_003775"
     tRNA            657..741
                     /locus_tag="KPK24_003775"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:Tyr,seq:gta)
     gene            751..824
                     /locus_tag="KPK24_003776"
     tRNA            751..824
                     /locus_tag="KPK24_003776"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:783..785,aa:Gly,seq:tcc)
     gene            835..911
                     /locus_tag="KPK24_003777"
     tRNA            835..911
                     /locus_tag="KPK24_003777"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:869..871,aa:Arg,seq:tct)
     gene            921..996
                     /locus_tag="KPK24_003778"
     tRNA            921..996
                     /locus_tag="KPK24_003778"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:954..956,aa:Gln,seq:ttg)
     gene            1008..1083
                     /locus_tag="KPK24_003779"
     tRNA            1008..1083
                     /locus_tag="KPK24_003779"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1041..1043,aa:Lys,seq:ttt)
     gene            1086..1174
                     /locus_tag="KPK24_003780"
     tRNA            1086..1174
                     /locus_tag="KPK24_003780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1120..1122,aa:Ser,seq:tga)
     gene            1192..1282
                     /locus_tag="KPK24_003781"
     tRNA            1192..1282
                     /locus_tag="KPK24_003781"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1226..1228,aa:Ser,seq:gct)
     gene            1291..1367
                     /locus_tag="KPK24_003782"
     tRNA            1291..1367
                     /locus_tag="KPK24_003782"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1325..1327,aa:Pro,seq:tgg)
     gene            1453..1528
                     /locus_tag="KPK24_003783"
     tRNA            1453..1528
                     /locus_tag="KPK24_003783"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1486..1488,aa:Trp,seq:cca)
     gene            1589..1665
                     /locus_tag="KPK24_003784"
     tRNA            1589..1665
                     /locus_tag="KPK24_003784"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1623..1625,aa:Pro,seq:tgg)
     gene            1672..1748
                     /locus_tag="KPK24_003785"
     tRNA            1672..1748
                     /locus_tag="KPK24_003785"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1706..1708,aa:Ile,seq:gat)
     gene            1752..1827
                     /locus_tag="KPK24_003786"
     tRNA            1752..1827
                     /locus_tag="KPK24_003786"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1785..1787,aa:Phe,seq:gaa)
     gene            1835..1911
                     /locus_tag="KPK24_003787"
     tRNA            1835..1911
                     /locus_tag="KPK24_003787"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1869..1871,aa:Met,seq:cat)
BASE COUNT          527 a          454 c          538 g          501 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaatgt
      121 ggttataata gcggaggaga tacacctgtt tccattccga acacagaagt taagttctct
      181 agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc acgtgaatgt
      241 tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc
      301 ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc ctttcacggc
      361 ggtaacaggg gttcaattcc cctacgagtc accatttgtg ggatcatagc tcagctggga
      421 gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc caccatttgc
      481 ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg agggttcgag
      541 ccccttccag gtcgccaaaa taaaaatgct ggtgtggctc aacggtagag cagctgactt
      601 gtaatcagca ggttgtaggt tcgattccta tcaccagctc catgtaaaac taatacggag
      661 gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt
      721 cgaatccacc atcctccacc aattaaatat gcgggtgtag ctcaatggta gagttctggc
      781 cttccaagcc agctgtgagg gttcgatccc cttcacccgc tccaaagaaa gcgtgggtgc
      841 atagctcagc tggatagagc aacgcccttc taaggcgtgt gtccggggtt cgaatccctg
      901 tgcgctcacc acatatatat tggggattag ccaagtcggt aaggcacatg actttgactc
      961 atgtatgcgt aggttcgagt cctgcatccc cagccaaata aaaatatggt ctattagctc
     1021 agtcggtaga gcacctgact tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca
     1081 ccaatggaga ggtgtccgag tggtttaagg agctggtctt gaaaaccagt gacttcgaaa
     1141 ggggccgtgg gttcgaatcc caccctctcc gccaaaaaaa tgactgaatt tggagaagta
     1201 ctcaagtggc tcaagaggat cccctgctaa gggattaggc tgggtaaccg gtgcgagggt
     1261 tcgaatccct ccttctccgc cattcaaaaa cgaggtgtag cgcagtttgg tagcgcacat
     1321 ggtttgggac catggggccg ggggttcgag tcccttcacc tcgaccaact taagctttta
     1381 gttcagttgg ttagagtgcc caactcatgg ctagtaattc tagggtttga gtccatgaag
     1441 gttattcttc ataggggtgt agctcagttg gtagagcgtc ggtctccaaa accgtgcgcc
     1501 gggggttcga gtccctccac ccctgccaaa taaaaaggac ttgaaaaaag ttggatttta
     1561 gagtagtata agatagtaaa taagatttcg aggtgtagcg cagtttggta gcgcacatgg
     1621 tttgggacca tggggccggg ggttcgagtc ccttcacctc gaccaagaaa agggcctata
     1681 gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc gctggttcga gtccagctag
     1741 gcccaccatt tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg
     1801 gtggttcgat tccgcctctg ggcaccagga atacggcggt atagcttagt tggctagagc
     1861 gttcggttca tacccgaaag gtcataggtt cgactcctat taccgctacc atctaactat
     1921 atcaataaag aactttgaaa attaaacagt aggttaattt ataaacaaga aacaaaccat
     1981 aaagccagat attttgataa caatagtatc tgagcctgat
//
LOCUS       DAEMGG010000247         1805 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEMGG010000247 DAEMGG010000000
VERSION     DAEMGG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.250"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..92)
                     /locus_tag="KPK24_003788"
     CDS             complement(<1..92)
                     /locus_tag="KPK24_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301394.1"
                     /translation="MKDKWILCPVCGNKTRLKIRADTELKNFPL"
     gene            complement(131..418)
                     /locus_tag="KPK24_003789"
     CDS             complement(131..418)
                     /locus_tag="KPK24_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301395.1"
                     /translation="MSTLIIGFIVAGVLLIAEYLLCTKFRSPLWGGIIPLLILAGTIY
                     VFASGRIPFERPYIFPFVVVNTLFFGDWGTGRDKYKKIKQTEMEKMKAKDI"
     gene            complement(421..717)
                     /locus_tag="KPK24_003790"
     CDS             complement(421..717)
                     /locus_tag="KPK24_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HBG2301396.1"
                     /translation="MELDKIPQWILALEQEDATFLKNFALKSGSLKEIAKMYGVSYPT
                     VRLRLDKLIQKIEMCDQQEEEPFQTFIKGLAVDSRIDVETAKIIIEKYKKEKEK"
     gene            complement(806..1711)
                     /locus_tag="KPK24_003791"
     CDS             complement(806..1711)
                     /locus_tag="KPK24_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2301397.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKSVIALWVVLIASVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQALNTDTIRKDIPVSSAVNGVQIWSVTQADENQFSVIFTVEQLITENGSTKT
                     VRSAYEVTVYVDGSGNMVLTKNPTITSIPVKSGYTPKAKESDGTVDAVTTDEINEFLT
                     TFFKLYPTATAKELSYYVNEGVLKPIGKDYVFSELVNPIYNRIDSQVTVSVAVKYLDQ
                     QTKATQVSQFDLVLEKSGSNWKIIK"
BASE COUNT          485 a          397 c          300 g          623 t
ORIGIN      
        1 aatagtggga aatttttaag ctctgtgtcc gctctaattt tcaacctcgt tttattaccg
       61 caaactggac ataaaatcca tttatctttc aataatgcgt tcctcctatc taagtataca
      121 gttttgtaat ttatatatct ttagctttca tcttttccat ttcagtttgc tttatctttt
      181 tatatttatc cctgccagta ccccaatctc caaaaaataa agtattgact acaacaaacg
      241 ggaaaatata tggtctttca aatggtattc tgccacttgc aaaaacatat attgttcctg
      301 ccaagataag caagggaata atacctcccc aaagaggact tctaaatttt gtgcagagaa
      361 gatactctgc aattaataaa actcctgcaa caataaagcc aataattaat gtactcatgg
      421 ttatttctcc ttttcttttt tatatttttc aataattatc tttgcagttt ccacgtcaat
      481 tcgagaatca accgctaacc ctttaataaa agtttggaaa ggttcttctt cctgctggtc
      541 gcacatttca attttttgaa ttaatttgtc taaacgtaat cgaacagtag ggtaagatac
      601 cccatacatt ttcgctattt cctttaatga accggattta agtgcgaagt ttttaagaaa
      661 agtagcgtcc tcttgctcta aggcaagaat ccattgaggg attttatcaa gctccaaaaa
      721 cacaactcct ttcatattat ctttaacaat attcagtata tcataaataa aattaaagtc
      781 aataagaatt taaacatcaa aataattatt ttataatctt ccagttgctc ccgctttttt
      841 ccagcaccaa atcaaattgt gatacctgcg ttgcctttgt ctgctggtcg aggtacttca
      901 cagccacgga aaccgtcact tgactgtcga tgcggttgta gatgggattt accagttccg
      961 agaatacata atctttcccg ataggcttta acacgccctc atttacataa taggacagct
     1021 ctttagcggt tgcggttggg tagagcttaa agaacgtcgt cagaaattcg ttgatttcgt
     1081 ccgtcgtcac agcgtccacc gttccgtcgc tttcctttgc ctttggcgtg tagccggatt
     1141 tcacgggtat gctggtaatg gtcggattct tcgtgagaac catgtttcca gagccatcca
     1201 catagaccgt gacttcatag gcggagcgta ccgtcttcgt gcttccattc tccgtgatta
     1261 actgttccac agtgaagatc acggagaact ggttttcatc cgcctgcgtg acagaccata
     1321 tctgtacccc attgaccgcc gaagacactg gaatatcctt gcggatggta tcggtattaa
     1381 gtgcctgcaa ttcctttgtc agataaccgc tgattgcctg cgtccttgct tcaatagctt
     1441 ctttgctgtt attccatgtg taataggact tcgcaaagtt cttcacgaaa ttttcaatcc
     1501 cgttggtatc ggtaagtctt aactgtatgg tttctgtttc atgtacggtg tgcatatcaa
     1561 tagcggtaaa attcttatat accccaaaac tcacgcttgc gataagcact acccacaatg
     1621 caatcacaga ttttttatgt gtaccgactt tcacagtacg cactttcttt tctttgacat
     1681 tctctgtctg tttcttattc ttcttaaaca tattttctaa gtcctttctt ttattttact
     1741 cgtcctgcac cgattaagtg ttgttgccag taactattat ttaagtctgc atatcctatc
     1801 ggatt
//
LOCUS       DAEMGG010000248         1772 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEMGG010000248 DAEMGG010000000
VERSION     DAEMGG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.251"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            491..1696
                     /locus_tag="KPK24_003792"
     CDS             491..1696
                     /locus_tag="KPK24_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG2301398.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
BASE COUNT          653 a          224 c          230 g          665 t
ORIGIN      
        1 ctcagatact attgttatca aaatatctgg ctttatggtt tgtttcttaa tataaattaa
       61 cctactgttt aattttcaat gttcgtgttt atttcttttt ccttttttca aggacaagta
      121 atactatatc atctttatct attctagtca atataatttt ttactttttt taattttttt
      181 atttatcatg tattctttat ctttaatttt taaaacttaa tgctaagtaa acctgtttat
      241 ttcataaaac taatatatat tctatgctct attttccttt ttattctata tttaaatttt
      301 ttatttacaa gaatattttt aatataacat atttgataaa attaatttac atttctttca
      361 cttgtaattt ttgcaatctt gtattagatt aatattaaaa ataatttatc aataacctta
      421 ttcacaaaga cagtttttat atatcaaaat ataatataaa tactatgcta cattttagga
      481 ggattttatt atgagcaaaa aagtattaat aatgtctgct tccactggtg gtgggcataa
      541 tagggctgct ctagctatta aagaagaact tacttccaaa acacttgatg gagagccaat
      601 tgaatgtgaa ataatagata gtttgaaatt agttaataat acaatggata aaataatttc
      661 aagaggatac gaaaaatctg ctatatatac tcccaaagcc tatggaagtg tatatagatt
      721 atctgaaaca aatcttttgt caaaaaatga atttaaagat aatctactaa taacattcat
      781 ggctaaaaaa tttaaaaagc ttattagaag cgaaaaacca gacttgatta taggaacgca
      841 tccttttcca atgattgctc taagtacatt gaaaaaaaac tttaatttac ataataatga
      901 atcaaatgct tacacagagc atttttataa acattataca aataccatta atgttcctcc
      961 gcttatttct gtattaaccg attatactac tcattctaca tggatacaaa atgaaattga
     1021 ttactatatt gttggtcatg aatatgtaaa agaattattg gtgtttgatg gagttgaacc
     1081 gagtaaaatt agaacatttg gaatccctgt tgaaaaatct tttctctctc acagagataa
     1141 agatattgtt ctttctgagt tgaatttatc tcctgataaa ttaactgttt tacttatggg
     1201 gggtagcttt ggggctggaa atataaaaga gacacttgat gaattactgg atactgatag
     1261 agactttcaa atacttgtaa ttactggtaa gaatgaaagc ctaaaagaaa aaatcgaaaa
     1321 aaaacttatg tctcgttatc atgataaaaa tgtatgtgtc cttggataca ctgataagat
     1381 gaatgatatt ttagcttcta tagatgtatt agtaagcaaa cctggtggac ttactacaac
     1441 tgaggctctt ttaaaagatg tacctatgat agttccttat tatataccag gtcaggaaga
     1501 agagaatctt gatttcttat ctaattgtgg tgcagcactt agaactacaa aaaaatataa
     1561 cttgtcaata ttactaaaag ttttaataga tgaccctagc agattagaaa tgctaaaaag
     1621 aaatattaaa tcaataagaa agtcaaattc agcccaaaat atagctaatt taattttaaa
     1681 tataatttct ggttgataaa ataaataaaa tccctatagt atatatatta tctattaggg
     1741 attttttatt ttctaattat ttaatattaa at
//
LOCUS       DAEMGG010000249         1760 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEMGG010000249 DAEMGG010000000
VERSION     DAEMGG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.252"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(100..1491)
                     /locus_tag="KPK24_003793"
     CDS             complement(100..1491)
                     /locus_tag="KPK24_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG2301399.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
BASE COUNT          626 a          319 c          189 g          626 t
ORIGIN      
        1 ttttttcata atcaaagagg aaattacatt taattttata tttaaatgta acttccttta
       61 tttaaagtaa ttcatttatt tacaataatt cgtcatgaat tatttttctt ttgttagtaa
      121 atccatagcc attaatcttg ttaatacatt tcttattccc aataatatta aaccaaatat
      181 tattaaaact gttgctattc ctccaaatat ctgtaaaaaa ccaagtgtgg ctgtaaaacc
      241 agcaatcttt cctgataata aacttgcaaa aaaacttgtt agataccagg ctcccataga
      301 caacgtagca tacttagctg gagctaattt attgaacatt gcaagtccta taggagataa
      361 gaataattca cctactgttg taaatatata agtcaatact ataaacaata tactagcctt
      421 taccattcca tctggaccat tagctaatgt agcaaatacc ataactataa acccaacacc
      481 cattattatc attcctaatc ccattttaac tggaatagag aaatcacctc ttttggtatg
      541 agataattta acccataggg tagctaatac tgggcataaa gccacacata atatagcatt
      601 tagtgatgta agccatgcta ctggcatttc aaatccaaat agagttctat ttacattatc
      661 ccttacataa atcataaaag aactttgtgt ttgatagtat gctgtccaga ataagaccac
      721 aaataaaaat aaaataacca ttgctattat cctatttttc tccaacttag taagtggtcc
      781 atactcaatt ttttcacctt tattaccttt agtcacagga tattttccga cttcatttaa
      841 taattttggt gcaagaagtg taaatataat catacctaac atcatgccta taccacaaat
      901 taaaaatgcc atcttatatc cataagatac tatatctcca cttgctcctc ttactgcaaa
      961 tacattatca gaaagcaatc cacatattat tggagctata aatgctccta catttactgc
     1021 catatagaat aaactgtatg ctgcatcttt tcttgacaca tcagttttag gatatatatc
     1081 cccaactata tttgagcaac ttgatttaaa aaaaccattt cctaaaataa ttattgatag
     1141 acctacccaa accattgcaa tagatgtaga tgaagcaaat agtacgaaat ctcctattgc
     1201 tattaaaagt gcacctatag aaatacattt ttgttgacct aaaactttat ctgctaacca
     1261 gccaccgact aatggtgtaa gatatataag acctgtaaat aagccaaaca tggatgttgc
     1321 ttcttgagta gaaagaccta aaccaccttg agctacactt gctgtaaaaa ataataccaa
     1381 taaagctgtt aaaccataat aactaaatct ttcccacaaa attgtcatgc aaataagcca
     1441 aaaactcttc ggatgaccac tttttttaga tacgctttct gtacttgaca taaaaaaacg
     1501 cccccttttg ttttatagtt aaatttaata ttaatattgc tttaaccatt ttagcacata
     1561 cttataatat catcttttct atgtttactc aatagattat cgcctgtatt ttcatttttt
     1621 tattattatt tttaaaatta tgaaaaatat tattttttta gtatcttcag cagaaaatct
     1681 taatatatat atatttaaat aacaacttaa atcttcaatt cttttttgtt tgttttttca
     1741 tttttgtaaa tttttattga
//
LOCUS       DAEMGG010000250         1723 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEMGG010000250 DAEMGG010000000
VERSION     DAEMGG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.253"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..218
                     /locus_tag="KPK24_003794"
     CDS             <1..218
                     /locus_tag="KPK24_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG2301400.1"
                     /translation="GYSTTARSMLLIVSLILTNIIPIGIISTRVDLSNNIILFGLLIL
                     VIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            223..840
                     /locus_tag="KPK24_003795"
     CDS             223..840
                     /locus_tag="KPK24_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2301401.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNKKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            1029..1373
                     /locus_tag="KPK24_003796"
     CDS             1029..1373
                     /locus_tag="KPK24_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG2301402.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT          726 a          163 c          249 g          585 t
ORIGIN      
        1 ttggttattc aactacagct agaagtatgt tgttaatagt ctcattaata ttaacaaaca
       61 ttattccaat aggcattata tcaacaagag tagatttaag taataatatt atcttatttg
      121 gacttctaat attagttata gaagtaattg tatctattgt attagacaag attatctcaa
      181 atagttcatt taataaaatt ataaaaggag aacattaaaa agatgatttc attaataaat
      241 atatctaaaa aatttaaaga taaatctata atagacaaat ttaacctaga aatttctaag
      301 ggcgagtttg tagcgataac aggaaatagt ggttcaggta agacaacttt acttaatata
      361 atgggattat tagaaaaacc taattctggt gatattataa taaataatat aaaaaatcca
      421 aatagtaaac agataagaat tttacaaagg gatttttatg gctatctatt tcaaaattat
      481 gctttaattg agaatgaaaa tgttgaaaat aacttgaaaa ttgctttaaa gtatcaaaaa
      541 aatatcaacc ataataaaaa aataaattac gcacttgagt ctgtaggtct tagaaaatac
      601 aataaaaaaa agatatacga attgagtgga ggagaacaac aaagggtagc attagcaaga
      661 gtaatattaa aagaatgtga agtaatattt gcagatgaac ctacaggtaa tttagataaa
      721 aaaaatagag acatagtatt tgatatactt aaaagtctaa ataagaatgg taaaactatc
      781 gtatttgtaa ctcatgattt agagttatcg gagcaggcag atagagttat aaaattgtaa
      841 taatatcaac tataataaat ataagaaaga gattacagct aaatcaagtt taataataat
      901 acgaatagaa tttaaacatt aagtaaatta gtagtttgta aatataagta taatatgata
      961 tcataaaggc tcaaaagaaa ttttatataa taagaaatct aaaaataaca aatggaggac
     1021 atataaagat ggaaaattta ccaaattgcc cgaaatgtaa ttcagaatat acttatgaag
     1081 atggaactct tttagtttgt ccagaatgct ctcatgagtg ggcattagga ttgggagctg
     1141 aagaagattc aaatattatc aaagatgtaa atggaaatgt attaaatgat ggagattctg
     1201 ttacagtaat tagagacctt aaagtaaaag gaagttcatc atctataaag attggaacaa
     1261 aagtgaaaaa tatccgttta atccatgact catcagatgg acatgatata gaatgtaaga
     1321 tagatggttt tggagctatg aagctaaagt cttctgttgt taaaaaagcg taatattata
     1381 aataagctgt agtgtttatt gtttttcagt acagtataaa accataagaa tctattaagt
     1441 aataaaagtt tgtttattta tctttatatt ttcttatggt tttattaaat ataaatttta
     1501 ttgaacacca aaatattctc tcctctacaa ataagccaat cttatttaaa ttaatatact
     1561 ttgtactatt tatactgaaa ttgtaaattt aaatgctatg ttatttaggt ttgtatagtt
     1621 aatttatata tttctgattt tataaattgt atttattatt atatcttgat tctaaaatca
     1681 ttaaaaattt caacttctat aaagatttac aatttatcaa ata
//
LOCUS       DAEMGG010000251         1682 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEMGG010000251 DAEMGG010000000
VERSION     DAEMGG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.254"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            540..1247
                     /locus_tag="KPK24_003797"
     CDS             540..1247
                     /locus_tag="KPK24_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2301403.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          709 a          150 c          282 g          541 t
ORIGIN      
        1 atatgcacta aattaaataa gttaattagt atataggtta aagtggaagt aattaaaact
       61 tattaagtat tagaaaatga cttcttatta gagtaagtat ctaaaatgat ataaaaatta
      121 tgattactca ttaaagtgac tattatagag tggataagat tactcattaa agcgattact
      181 gtggagtaga taaaattact cactaaaata actatcatag agtggataag attactcatt
      241 aaagcgatta ctgtagagta gataaaatta ctcactaaaa taattatcat agagtggata
      301 agattactca ttaaagcgat tgctgtggag tagataaaat tactcactaa aataactatc
      361 acagtagata agattattca ttaaagttac tatcacagag tggataaaat tacacattaa
      421 aattgtctgt taaagaatga ataaaaaaga gattgctcaa tataagaagc atataaaaaa
      481 taaaaaatca aacgtataaa atttaataaa taataactga tagggaaagg aaagatagaa
      541 tgatatttac aaaaagtaat aaatacgata aggactttct catgaaaaat atgatgggac
      601 ctaattgtat aaaaatattg gaagaattaa caagtaagat aaaactagaa aagggaatgc
      661 gtattttaga cttaggttgt ggtaaaggta tatcttcgat atttttagca aaggaatttg
      721 atgctactgt atttgcaaca gatttatgga tagaaccaac tgaaaactat aaaagattta
      781 aagagtttaa attggatgat aaaatatttc caatacaggc agaggcacat gaactacctt
      841 atgcagaagg tttctttgat gcggtgataa gtatcgattc ttatcactat tttggaaaca
      901 aagaaggatt tttagacaat catatatcac ctcttgtaaa agagggggga atacttgcta
      961 tggcaatgcc tggattaaaa gaagatttcg tggattgtat accagatgaa ctcattccat
     1021 tttggcaaga taatatgaac ttccattcaa ttacatggtg gaataaactt tggtctgaat
     1081 cggagtctgt gattgtagag aaatgtgaag ctttaaattg tcatgatgaa gcttggaagg
     1141 attggataaa ttgtgataat tcatatgcta taaatgacaa aaaaatgatg gaagtagaga
     1201 atggaaagta ttttaataca atttctttga tagcgagaac taaataattg tgatgaattt
     1261 ataaaagtat aagataagta gaaatataag ttgaatgaga gtataagaag catagaaata
     1321 taagttgaat taaagtagaa ggtaaataaa aatgtaagtt aaataaagag tttatacaaa
     1381 aatgattttt tagattattt atacccaatc gagttgctta ggtcagttgg gtatttttat
     1441 tttttaaagt taaattgcaa atactattgt gtgatatata ttttaatact aaatgtttgt
     1501 tttataagaa ataaactaat agaaaaaata gttttaacaa tgaaagcaaa aaaggtattg
     1561 atgaagatta aaaattttat agtaccattt tatatatttt attatacttt aaatcaaatt
     1621 tcaaaatgat taagtatata tacccaaaag attaaagaac tatatttatt atactattaa
     1681 aa
//
LOCUS       DAEMGG010000252         1657 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEMGG010000252 DAEMGG010000000
VERSION     DAEMGG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.255"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            139..1377
                     /locus_tag="KPK24_003798"
     CDS             139..1377
                     /locus_tag="KPK24_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG2301404.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          707 a          141 c          276 g          533 t
ORIGIN      
        1 atagatattt taaggtatta gttgttaaat taggaaatat taaaaaaaat atatatttat
       61 atttaaacta ttttgttatt tatataaaaa tacttatcta gttactagca attattagta
      121 gaaatggggg aaatagagat gaataaggga ttttacaaat taaattcagt ggtattgaac
      181 tttgatatta agaatgatgt taacaagata agatttaatc attatataat gagaaaagaa
      241 aacatacaaa gacttcaagg aactctgcca aacggacaat tttatatgac agtgagaaga
      301 gctgctacag atttacaact ttctatttct actataagta ggcttgtaaa agagtttttg
      361 gatttaggga taataagatt agtgtctaag ggtgtaaaag gtaatcgttc tgtatatagt
      421 tacgtaagta tagagtttaa tggaagttta tttgatgata gcaaatatat ggaacttgac
      481 tgtgtatgta atgatgaatt tattgataat agtggcatag agtttgattg tgatagcaaa
      541 gataattctt ttaatggaga tataaatgta gaaactgtgc attgcattaa tgagtatttg
      601 tctgatgatg atgtagagca taattataac aatttccata ataaagatac tataaaggat
      661 aaagccattt ctaatattag cactatcaaa aaatatattg atgaatcttt tgataaaaat
      721 aaatcagaaa attctaactt acaacaggag tcaataaatt tcaactttaa tgatacatgc
      781 aatgaaatag ataaaagaac gaataatagt atagaaaata atcaaaataa aaggcctatg
      841 agattgagtg acatcagtct tttaagtgat aaaggtatga caaataaaaa gaattacttt
      901 aatcataatc atggtataca aaaagggact aaatctgtaa caaagaaaaa agaatcctta
      961 aataaattta ataaaaaaaa tatacagcaa agcattataa atatgctaaa taaaaaatca
     1021 ggtaagaatt ttaaaatcga ttctccaata agcataaaac ttatcaatga aaggctaaag
     1081 gaaggctatg tcttagaaga tttctacaaa gttattgagg taaaagtggc taagtggaaa
     1141 aataccataa tggaaatgta tataagacca gaaactttgt ttagctataa gtttgaatcc
     1201 tatgtaaatg aaaacatatc tatgaaaagt caaagtaatg ttcacccatg taaagaaggc
     1261 aatagtaatg atagcagtaa ttatatacat tctgattata caacatatga tggcagtgtg
     1321 cgtgagagaa aaaaatatga gaatgtaaga tactggaatc ctatatgtga aatttaatta
     1381 gtttaagaat gtatttatga taaataagat attaatagag aatagtgagc tatagcaaat
     1441 gatatatcaa tagttggtag taagttatat taagcaagat attactatca ggtagtgaat
     1501 tatattgaac gatatatcaa taattggtag taaattatat taagtaagat attactatca
     1561 ggtagtgaat tatattgaac gatatattaa taattggcag taaattatat taaataagat
     1621 attaatatcg agtaatgaat tatattgaac gatatat
//
LOCUS       DAEMGG010000253         1643 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEMGG010000253 DAEMGG010000000
VERSION     DAEMGG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.256"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            297..1568
                     /gene="serS"
                     /locus_tag="KPK24_003799"
     CDS             297..1568
                     /gene="serS"
                     /locus_tag="KPK24_003799"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG2301405.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKTEYVHTLNGSGLAIGRS
                     VAVILENYQQEDGSVVVPEVLRAYMGVSVIK"
BASE COUNT          693 a          168 c          296 g          486 t
ORIGIN      
        1 ataaaagtta ataactatac aagattgata aagaaaaagt caaaatagat aattgattag
       61 caaaatagat ataattataa tgaagttaaa tctagcaata ttctcctaaa attaaatttc
      121 atttatttaa ttaaatataa tttgacaatg ggcaaaaaag tgagtaaaat ataaataaaa
      181 taaaaataac aggttcggat aaagaaaaag gtttcttaac ctcaagcata aagtatatta
      241 tctaagttat ttgagattaa gagataaatc caatcttagg tactgaggag gaaaaaatgc
      301 ttgatataaa aagaataaga gaaaatttag attgtataaa aaaagcaatg gaaagaaggg
      361 gcgaaaaaga ctttaacctt gatgaagtag ttaagttaga tgatgaaaga agaaagattc
      421 ttcaagaagt tgaagttatg aaaaatgaat taaatacagc atctaaaaat ataccaaatc
      481 ttataaaaga aggtaaagat gtagaaaatg aaaagattaa actaaaagag ttatctgata
      541 aaattaaggt aatagaccaa aatcttaaag aagtagaaga taagatggaa tacctattaa
      601 tgagaatacc aaatgtacca catccagaag taccacaagg tgaaactgat gaagataatg
      661 ttgaagtaag aacttgggga aagactacta cctttgattt tgaatctaag gctcattggg
      721 aaattggaac agaattaggt atacttgatt ttgaaacagc tagtaaaata actggttcaa
      781 gatttactct ttacaaagga ttaggtgcta gattagagag agcattatta aatttttact
      841 tagacacgaa tacaaaagta aatggatata cagaagttat tccaccattt atggcaaata
      901 gaaatagttt tttaggtaca ggacagttac ctaagtttga agaagatatg tttaaaatag
      961 aaggtctgga ctattttatg attccaacat cagaagttcc tttaactaat atacatgcta
     1021 atgaaatact taaatttgaa caattaccaa taaattatac agcttatact ccatgtttta
     1081 ggtcagaagc tggttctgct ggaagagata caagaggttt agtaagacag catcagttta
     1141 acaaagtaga aatggttaaa atagttgcac cagaagaatc ttacaatgag ttagaaaagc
     1201 ttactaacaa tgcggaaact atgctacaat tattgaatct gccttataga gtagtaaaga
     1261 tatgtacagg tgatttagga tttacggctt cttttaaata tgatgtagaa gtttggatgc
     1321 caagttacaa taggtatgtt gagatttcat cttgttcaaa ctgcgaagat ttccaagcta
     1381 gaagagcagg aataagattt aaaagagata aagattctaa aactgaatat gtacacacat
     1441 taaatggctc tgggttagct attggaagaa gtgttgcagt tatattagaa aattatcaac
     1501 aagaagatgg ttctgtggtt gttcctgaag ttttaagagc atatatggga gtttctgtta
     1561 ttaaataaga gttaaattat gtatttttaa gcaatacaat aatatagagg taataaaaaa
     1621 taattaatat aaaagtgata ctt
//
LOCUS       DAEMGG010000254         1641 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEMGG010000254 DAEMGG010000000
VERSION     DAEMGG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.257"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            93..827
                     /locus_tag="KPK24_003800"
     CDS             93..827
                     /locus_tag="KPK24_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2301406.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNALMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(970..1461)
                     /locus_tag="KPK24_003801"
     CDS             complement(970..1461)
                     /locus_tag="KPK24_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG2301407.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          610 a          195 c          241 g          595 t
ORIGIN      
        1 cttttattat tttatataaa agtgataaaa acttatattt tgtatttaat agagtgttgc
       61 taagtgaata tatttaaaat ggaggaaact ttatgaaaca aaataaatat gatgatgata
      121 gattttttaa taaatacagt aaaatggaac gttctacaaa tggattagca ggtgctggag
      181 agtggcatgt tttaaaaaaa atgttacctg atttcaaagg aaaaagagtt ttagatttag
      241 gatgtggatt tggatggcat tgtcaatatg cagttgagaa tggagcaatt tctgctgttg
      301 gagttgatat ttcagaaaaa atgttaaaag aagctagaaa caaaacgcaa cttgataata
      361 taaattacat atgtatgcct attgaagata ttaattttcc aaaagatagt tttgatgtag
      421 ttattagttc cttggcattt cattatattc aatcatttga agatatatgt aagaatataa
      481 gtaattgtct ttcaaataaa ggtaattttg tattttctgt tgaacatcct atatttactg
      541 catatggaag tcaagagtgg tactgtgata aagatggaaa taatctacac tggccagtag
      601 atagatattt tgaagaagga gtacgtaggt caaattttct aggagaagag gttataaaat
      661 atcataaaac attaacaact tatttaaatg cgttaatgaa aacaggattt gaaatattag
      721 aaattattga acctcaacca gaagaaaaac tgttagatac aattcctgat atgaaagatg
      781 agctacgtag acctatgatg ttacttgtat ctgcaaaaaa aagataagta aatataaata
      841 atcattattg tagaaaaaaa gttgtagaaa ataattacac taatggagat gattatacca
      901 gtggtattaa ttacaccagt agtaaggagg caaaagcatt cgttgctttt gccatttttt
      961 aagttgcaat tatacttcta cgtagtatat ttcgtttcca ttttcttcta taagcttttt
     1021 catatcttca aattttaaaa atacagtagc agtattatca tttggatgta tacctatttt
     1081 atccattttt aataaatcct tatcaaatac aacttttaca acagcctctt tatcatttaa
     1141 tataccaaaa ggagtcacag ctcctttagt aagtttcaag tatttttgaa gtctttcttc
     1201 tgatgcaaaa ctaagaggtt tactatcaat ttgttgacga atatctttta aatctgcttt
     1261 tttgtcttta cctaatacaa ctaaaaaatg ttcttttcct ttagcatcac gaagaaatag
     1321 gttttttaca acaaattcca tatgtaatgt ttttaattct tccatttctt caattgtata
     1381 aactggtgga tgatttacag cctcataagg tatgcttaac tcatctaatt ttttatatac
     1441 ttcctgttgt tgattattca tgatttcctc ccattagaaa tttattttat atataaataa
     1501 tattatcaca gaaaacttta aaagaaaacg ttttatataa tatagacaat agattatatt
     1561 tttataaaga agcaagaata ctcaataaaa cttaaagaaa ttaaacgatt tctaaaaata
     1621 ccttgaaaat agaaccttat t
//
LOCUS       DAEMGG010000255         1561 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEMGG010000255 DAEMGG010000000
VERSION     DAEMGG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.260"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            521..988
                     /locus_tag="KPK24_003802"
     CDS             521..988
                     /locus_tag="KPK24_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2301408.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDYFDTFVERLSTMRDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          629 a           99 c          221 g          612 t
ORIGIN      
        1 taaatttatt taaaattaaa attttattta ttattaaaga gtaaatataa ttattagtaa
       61 tatatatata gtatttgtaa taaagtatca ataattagaa agattatgaa aactaaatta
      121 gaagaactaa aaactatatt tttattaatt agaatatttg aataatctct ttaatcatat
      181 aaaatacata tgcttgtaat atttaaaggt gatattaata ttattagtgt atattaatat
      241 tctcttgaaa ttaaaaaaac atagatattt cagtaatcta aaatagtcta caaaaataac
      301 agttttagca aaattaataa gaatacatat agatttaaac ctaaaagtat gaatgtgtct
      361 gttttttaca agattaaaat ttatattata tgatacaatt aacttaacta tttcaataag
      421 acttataatt tttttcttta tactagacct tattgaaacc tttatagttt aattctaatt
      481 atatattata aataaagtat gtgtatatta aggaggtaat atgaagtttt taaatgtttt
      541 aatagtagtt gaagatattg agaaatcaaa aaagttttat tatgatgttt tggggttaaa
      601 ggtaatctgt gattttggag aaaatgttgt attagaaggg aatatatcat tacaagaaaa
      661 aaagctttgg ttagagttta ttaataagag tgacagtgaa gttaagttca atggaaatga
      721 tgctgagctg tattttgagg aagattattt tgatactttt gttgaaagat tgagtacaat
      781 gagagatata gattatgttc atctagctat agagcatagg tggggtcaaa gagcaattag
      841 attttatgat ttagatgggc atattattga ggttggagaa actatgtcat ctgtgtgtag
      901 acgtttttta gatagtggtc tttcaataga tgaggtagct aaaagaatgg atgtaactgt
      961 cgaatatatt gaatcagttt tagaatagat ggattatatt aaagtaaaac tatattttgt
     1021 atagaagtaa gggctgtaag taaacttgta tggaaacaaa tttacttaca gtttttgttt
     1081 ttgtaagata cagaatataa tatttgttat aattttattt ggatttttaa aaagtgcatg
     1141 attttcctta catattataa tagaatttaa tatttattgg atgatttaaa tttagaaaaa
     1201 tataagtatt agcttataaa agataattat acttactatc aagctgtaga atcatatata
     1261 ctattttatg taattatgtt aaaatttaat tgaacaaatt aaaaatatag tgaaaattgt
     1321 agttttagtt tatttattta ataaatattt caaataaaat aatgtttaaa taagaaacat
     1381 aaataataat attattattg ttcttaaaga caaaaaatct tagcttaaaa atttgtatca
     1441 tataaaatcc ctacatatta tggaagtaat gatacattat atgatataca caataaagta
     1501 taaaagtatt tacatgtttt ttctatatat tttataagtt ttaggtatgg aaaattacta
     1561 a
//
LOCUS       DAEMGG010000256         1422 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEMGG010000256 DAEMGG010000000
VERSION     DAEMGG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.261"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            218..595
                     /locus_tag="KPK24_003803"
     CDS             218..595
                     /locus_tag="KPK24_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2301409.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            complement(1032..1295)
                     /locus_tag="KPK24_003804"
     CDS             complement(1032..1295)
                     /locus_tag="KPK24_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301410.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
BASE COUNT          536 a          116 c          184 g          586 t
ORIGIN      
        1 tatgtcaaat attagaattt taatttgata atctgttata cttatagaat agatatttat
       61 ctttatatta atagattgtg tgtttaattt tgaataaaaa tataaatttt ttgtattaat
      121 aaatttggat gagatatttt aaataaaacc tttgactcga aaagtctatt aatatagtat
      181 ttaattatgt agaagtttga gaggagtgta aaatcatatg actattacta agataccacc
      241 agcagaatta aaagtcatga aatttatttg gagttcagat tttacagtag catcaaaaga
      301 tgtcattgaa gctatggaaa aactatataa ttggaagcaa actacaacac taacactttt
      361 atcaagatta gtaaataagc aattcttaag tgcaagaaaa attgatagac atacacatta
      421 tacagttctt attggagaag aagagtattt acattttgaa acaaaaactt ttttagataa
      481 tatgcatgga agttctattg agagttttat gaaatccttg ttcaaaaagg gggtaaatga
      541 agaagaattg aactctgttg aaaaatgggt taaggatatt aaaaaagatg aataatttgg
      601 tgtagttaat tatcataaaa taaatagttt ttacaataat tagttaaatg aatcaaatat
      661 aaagttttat gtaaaatttg tgatttttat aaatattttt gtcttgttat ttaatattaa
      721 tacaataagt ttgatgtaaa atcaaacctg tttgaattta atttatattg ggtttgtcat
      781 taatattatt ttagtttgat attgtatacg aatttaaaag tcagaatttt tgtatattaa
      841 tcatgtaaat taattgtttt agatataaag ggcataattt tatttttgtt ttgattattt
      901 ctagggtaag ataagtatac aatagattgt attaaacatg cttatattga tagatttgag
      961 ataaatattg agatatatta ataattatta atataaaaaa gttcctagtt tttaggaact
     1021 tttttatttg attaatacag agtatgagtt tttgcttcag ctatagctct tgctgcactt
     1081 ttaggttttt cagtattttc ttttttgtaa aatttaatca cttcttctaa atctttattt
     1141 tcaatattat gttttttgtt ttcaagaata taatttacaa ttgattcatt tatcaaatct
     1201 gtcaaaatat tattttcgtc attagttatc ttagacattt cttgtaataa aatttcatta
     1261 acttctactg taatttgttt tgtatttttt atcatatcaa ccacctctta agaaaattat
     1321 atcatctcaa aaaaataaat tcaatatgtt ttaaaattat agaattttaa ttcttaaatt
     1381 ctaaaaaaga gaaaattaga taaagtagtt ttagatttta aa
//
LOCUS       DAEMGG010000257         1407 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEMGG010000257 DAEMGG010000000
VERSION     DAEMGG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.262"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            148..639
                     /locus_tag="KPK24_003805"
     CDS             148..639
                     /locus_tag="KPK24_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301411.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            667..>1407
                     /locus_tag="KPK24_003806"
     CDS             667..>1407
                     /locus_tag="KPK24_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301412.1"
                     /translation="MRITKTTFNDVFSSDANESIIKVEKYVDKKCASVFEILTYTIIV
                     TNTNRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENI"
BASE COUNT          633 a          112 c          196 g          466 t
ORIGIN      
        1 tttacttgta aattgaaatc aatgaagtaa aaaaggattc tttcttaaaa gtaaataaca
       61 actagatagt gaataataaa aaatggtaat ttgaaataat ttttatgaat agtattaata
      121 tatagataca gggagattga tgcgattatg tatgaaaata ataaggtaat atacaacctt
      181 gaaagatgtg aaaaactaga aaagactctt tatttatctc aatttaatac atataaaaat
      241 tttaaattag cttctaaaaa aactgtaata aattcattag agacatatgt ttacaatatg
      301 aatattaaca atataagtct tgttgatact atagaatgta tatcattaga agatgaacaa
      361 cttacaggaa agaagttgat tttagttggt gatatagata ttgatgcaat attagattat
      421 gcaggaaata aaagaaatag aaattctagt aaaaaaaata catttaaatt aaaaataccc
      481 tttagtacat ttatacaaat gcctagaaag atagaaaata aagataaaat taatttaaag
      541 tatcttatac aagatattac ttcatctatt ttagatgata atttatttat tagtgttact
      601 gcaattataa gttatgaaaa tagttccaag atagagtaat ctttaaatta tagaaaggtg
      661 gatgcaatga gaataacgaa aactactttt aatgatgtat tttcaagtga tgcaaatgaa
      721 agtattataa aagtagaaaa atatgttgat aaaaaatgtg catctgtatt tgagattttg
      781 acctacacta ttattgttac aaatacaaat aggtacaaaa ctggaaatat atttttcaaa
      841 gattatattt ctaaatatat agagtttata aataatactg taaaagttaa tggcattata
      901 aaacgtggat tagacccaca gcaaggtttt tacataggga gaatagatgc aagttgtaaa
      961 aaaataatat cttttaagag tgtagtttta ccaaattctg cacataggat tataaagaat
     1021 agtgctaata tatactatta ttataagtgt aatctagata aattcccaac aagaatatca
     1081 atagaaagta atagagttta tactaatgta aataaaattg tattcaaaca attgaatata
     1141 agtaatactt taaaagctcc taaaaatcta agagatatat taaaagtaaa tgtaaatagt
     1201 aaaatatttg atgttaaacc tatgaaaaat cttataaaca aaggtagtaa tttgaaatta
     1261 tgtaacttga tagtttttgg gtgtatagaa gttgaaatag actatagttg taaaaatagg
     1321 agtaatttta aaacagtaaa taagtctaat gacattaaaa aagataagta ttataaaaat
     1381 gatatacaaa ataacaatga aaatata
//
LOCUS       DAEMGG010000258         1390 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEMGG010000258 DAEMGG010000000
VERSION     DAEMGG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.263"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            141..>1390
                     /locus_tag="KPK24_003807"
     CDS             141..>1390
                     /locus_tag="KPK24_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG2301413.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPM"
BASE COUNT          591 a          171 c          163 g          465 t
ORIGIN      
        1 agttttttat gtaggtaaca tttctacatt tttataaatt tttttacgtt ttttaatttt
       61 ataattaaaa atttaaattt tatacatatt ataataaatt gtgatatata taaaataatt
      121 aaagataaaa ggagtatttt atgaacatac ttggattaat agttgaatat aacccatttc
      181 ataatggaca catatatcat ttatctaaat ctatagaaaa aacaaatgca acacatactg
      241 tagcaataat gagtggaaat tttctacaaa gaggcgaacc agctttattt gataaataca
      301 ctagagcaaa aattgctgtc gaaaatggtg tagacttagt tatagaatta ccaactttat
      361 ttgcttgtca aagtgctgaa attttttctc atggtgctat aacaatttta aattcactaa
      421 actgcataaa ttctatatgt tttggtagtg aagaaggaaa tacagaaatt ctttataata
      481 tagctgaaat tttagcaaat gaaccaaatg aatttaagtt acttttaaaa aaatatttag
      541 atgatggtct gttatttcca acagctagag ctaatgcact ttttgattat atacatcaag
      601 acaaaaaaaa taattttata caaaattatt caaaggaaca attatcaaaa acattaaatt
      661 catcaaacaa catactgggt atagaataca taaaaaacct catccattta aacagtagta
      721 taaaaccatt tactatctcc agggtccaat cagagtataa ttccgaagaa attgagagtg
      781 atatttgttc tgctactgct ataagaactt cacttaaaga aaatacagac ctttttcatt
      841 taaaaaatgt tattccagaa aaaacttata acatcctaaa tgaaaaaata gataatggtt
      901 ttttacctat gtttgatgac ttcttctttg atattttaaa aacaattata cttagagata
      961 tggacacttt aaaagagtat tttgaagtaa atgaaggcat agaaaacaga atctataaag
     1021 atatctttac atctgaattt ctacatgaac tacaattagc aattaaatcc aagcgataca
     1081 ctttaaccaa aatcaaacga actctaaaca atatattgtt aggtattaaa aaaagcgaca
     1141 tgcaacttat aaaaaacata gatactatac cttatataag aatacttgct tttaatgata
     1201 aaggtagaga aattctaaaa gagataaaaa ataaatctga aataaaaata ataaacaagt
     1261 tttcaaatat atcattttca ttagatgata cgatttttaa aacactcata agttatgata
     1321 taaaagctac aaatatatat aattcaatat attataaaaa caataaagat ttagtaaaag
     1381 gccctatgga
//
LOCUS       DAEMGG010000259         1324 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEMGG010000259 DAEMGG010000000
VERSION     DAEMGG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.264"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            183..1280
                     /gene="ychF"
                     /locus_tag="KPK24_003808"
     CDS             183..1280
                     /gene="ychF"
                     /locus_tag="KPK24_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG2301414.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          515 a          144 c          241 g          424 t
ORIGIN      
        1 atttaaatta aatacaaata ttatttaaat caatatgctt taaagtctag tttacaagct
       61 tgatatatat gatgtataat caatatgtat gtctataatt aaaacaattt tgcaatccta
      121 tagattgatt gtttaaagtt atataaagaa tataaaaaag aataagagag aggacgatta
      181 atatgaaatt aggtatagtt ggtttaccaa atgtaggtaa aagcacacta tttaacgcaa
      241 taactcaagc aggtgcagaa tctgcaaatt accctttttg tacaatagac ccaaatgtgg
      301 gagttgtatc tgttccagat gaaagattaa acaaactaca agaattatat aattctgaaa
      361 aaatagttcc tactgctata gaattttgtg atatagcagg tttagttaga ggagcttcta
      421 aaggagaagg tttaggaaat aaattcttat cacatataag agaagttgat gcaatagttc
      481 atgttgttag atgttttgag gatgaaaatg ttgtacatgt agatggttct gtagacccac
      541 ttcgtgatat agaaactata aacttagaat taattttctc agatatagag attcttgaaa
      601 gaagaattaa taaaactcaa aaagctgcta aggctgataa aactttaggt tctgaacttg
      661 atttattaaa atcaataatg tctactttag aagaaagtaa gtgtgtaaga actatggaat
      721 ttacagaaga tgaacaaaca tttgtaaatt cattggattt acttacatct aagcctgtaa
      781 tatatgcttc aaatgtatca gaagatgatt tagctgataa tggagaaaat aataaatatg
      841 ttcaacaagt taaagcattt gcagaaacag aagatgccga agttgttgta gtatgtgcac
      901 aaatagaagc agaaatttct gaacttgatt ctgctgaaga aaagaaagag tttttagaaa
      961 cattaggtct tgaacaatca gggcttgata agcttataaa atcttcttat gctttacttg
     1021 gtttaatttc attcttaact gctggtccaa aagaagttag agcatggact ataaaagttg
     1081 gaagtaaagc tcctcaggca ggaggaaaaa tacattctga tatagaaaga ggatttatca
     1141 gagcagaaac aatagccttt aatgatttgg ttgagcatgg tactatggct gctgctaaag
     1201 aaaaagggtt ggttaaatta gaaggtaaag aatacatagt taaagatgga gatgtaatat
     1261 tatttagatt taatgtttaa aatgattaat cgtatacact ttgactatta tatataataa
     1321 ctaa
//
LOCUS       DAEMGG010000260         1307 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEMGG010000260 DAEMGG010000000
VERSION     DAEMGG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.265"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(223..714)
                     /locus_tag="KPK24_003809"
     CDS             complement(223..714)
                     /locus_tag="KPK24_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG2301415.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          509 a          171 c          111 g          516 t
ORIGIN      
        1 gataaataaa aatatctgca actatatatt gcaaaaattt aaaactaatt tatatatgct
       61 aatataaata tttataaaga cataagacaa atttacctac agatttaaat ttaacttata
      121 tattttaaaa ggctaatata atatgtattt tcttctatac aatcaaaagg cttcatatct
      181 aagatatgtg atgacactaa tattcacaat ttttagtaat tatcacatta taaaaaatat
      241 tacagctagt ccatcattag atgcttcttg tataaacttt ttaagctcaa ttagatttgc
      301 ccatacatat tcaaaaaaat catcttcact ctcatcattc ataacaggat aaatattatt
      361 ttctaacatc tcattaatat taaatctttt tctaaatgct tgttcttcca aactgttcat
      421 tgcttttgat agttttttta catcatttgc tgtaatcaac atgacaggaa attctccatc
      481 aatttccata agtatgtttc cacttagtac taatttgcta aatatattat catcatctcc
      541 accaaaagga caacctgtca aagtaaagtg aatggcatgc catgctttat caatattgat
      601 agtactattt tcgttggcat cttcaaaaac aaaatcttgt aaattttctt ctgcttgctg
      661 taatttaaaa acattctctt cacttatttt agtataacat cctatcattc ccataattac
      721 attcctccta agtagcgttt ttttaattgt aacttataat taaacttagt tacaataaaa
      781 tatattgaga aaagtattat ttcatcttaa atttaaatac ctatattatc aactaaaatt
      841 ctgtgtttat agcgtaagta ttattttttc ctaaaaatta aatgaatcag aggttctata
      901 ggcaatattg actataaata agaactatta ctttttaaaa aaaatttaag aaattatact
      961 gtagaatcat gctctactta ttagctttaa ataaaaaata aatcatgttc tcaaattaaa
     1021 taataaataa caataaattt aaattttaaa ctataagagt ttctgaagtg ttaataagca
     1081 taccaataat tatatgtgta agaatttgat ttaatagata tgaataattt ttacattaat
     1141 tataaatata ttaacctatt gttataaatt ctaaaaaaga aaagttatct taaataaaaa
     1201 ataagtgtat cttaaatttt aaatgataca cttattttta tattgaaatt atatttttta
     1261 atatctattc ttaattaggt atttttaaat tatctgttta cattaaa
//
LOCUS       DAEMGG010000261         1259 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEMGG010000261 DAEMGG010000000
VERSION     DAEMGG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.266"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            96..827
                     /locus_tag="KPK24_003810"
     CDS             96..827
                     /locus_tag="KPK24_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301416.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(984..1190)
                     /locus_tag="KPK24_003811"
     CDS             complement(984..1190)
                     /locus_tag="KPK24_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301417.1"
                     /translation="MSKYSSLWEYFKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          526 a          133 c          152 g          448 t
ORIGIN      
        1 attataatct ttagaacaaa cagtagtaaa tgttatcttc ataaacaaat ttatttttca
       61 caagtacaat acttatttaa aaaaggaggt ttaatatgtt aagtaaaaaa aatgtaagtt
      121 taattatttt agtaataatt ttaagtttat taactgctat atttattttc aataataaaa
      181 atactaacaa taaattgaaa gaagttacta agaataatga agaaatagta cctaaaaact
      241 tagaaggttt aagtatagaa caattaatag agattagaga taaagccatt gataaaaatt
      301 tagaaggtct tagggaattt ggagaagatt cagtaaaaaa acgtaaagat tcaattttaa
      361 actttgaatt tggattagta gcttttgtta acaagaatta tagcaactta acagaaaaag
      421 agaaattaat aagacaaatt aatattgtta actatgaaaa ctctataaat gatataatgg
      481 atccttcagg ttcattcttt gaattagcaa atcagaatta ccaagtgaat gagtttctat
      541 taaaaaacgg aaaagattat aaagaatggt catatgaagg tctagatttt ctaattgatc
      601 atctagaaga actgtgcaaa tttgttgatt ttgatgtaaa agatgtaata gatattattg
      661 atcctacaat aaaaacagac ctttcagagg aacaaaagaa atctttgaat gataaattaa
      721 agaaaatatc ttcaagtgat actttaagtg aagaagttaa aaaggagata aaaaactggg
      781 aaaacaattt aaataattta aatatgaata aaaataattc taattaattt tctcataaga
      841 aaaatgctta tatcgtgata atgtgaaatg tacataatta taaacaaaaa aggggctgtg
      901 acactaaaaa atagtgctac gccctttttt atttgtaata atcatcaata caaattattt
      961 tattatatgt aattatttat gtcttaacca attttatcga aaataacagt cttctctttt
     1021 aaagagattt ttcctacttg atatccatat tcatttaatt cctttttata tttcaaaaac
     1081 gaatggtcta tctctatccc agcaatatct tttatttctt caaaagttaa tttaaacgat
     1141 tgactattgt ttttcttaaa atattcccat aaagaactat atttactcat cttatatcac
     1201 tccttttaaa atcatatctt atctattaat ttatattcta tcattattaa tgtataaag
//
LOCUS       DAEMGG010000262         1244 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEMGG010000262 DAEMGG010000000
VERSION     DAEMGG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.267"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            118..1011
                     /locus_tag="KPK24_003812"
     CDS             118..1011
                     /locus_tag="KPK24_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG2301418.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT          522 a          126 c          186 g          410 t
ORIGIN      
        1 gcttttaatt aaatatattt cataaatctt cataaaatct tcaaaatttc gtaaagaata
       61 tgtcacaaaa gtaaggtata ttatattcag cagatagtat aagggaggac aatatttatg
      121 aagaataaaa aattcattgt tataatactg ataatatcaa ttttaatagg tgtattagtg
      181 aaggaatatt catctagaga gataaaaaag gatgatataa atgtcagtaa gtatataaaa
      241 tatgctgatt tagcaagtaa aaataatgca caagttaact ggaagtatgt agcttccatt
      301 gttgctgtgt taaataaaaa taacttaaaa aacgttaagg attcacagat acaagaagta
      361 tcagatttat ttgttaagaa tttttctaaa aataataaaa taaataagtt aagtgatatt
      421 ttggatgagt tagaatttag taatagacaa aaaagattgg ttgataatta tatagacaat
      481 cttaaggatt atggtataaa accagaaaga ttgaagtctg atacaaaata tatgaagttt
      541 attgatgaaa ttaaaacaga agcaatacaa aattataagg attataaaat acttccatct
      601 ataactattg cacaagctat aatagaatct agctggggca agtcaacttt ggcaaaacaa
      661 tatagcaatc tatttggtat aaaggctgac gcttattgga aaggtaagtc agtgacttta
      721 gaaacgaaag aacatttaga tacaattata gatgataaat ttagaatata tgatgataaa
      781 aatgaatcta taaaagacca tgcaaaattt ttagccacaa ataaaaggta taaaaacaat
      841 ggtgtatttg atgcaaaaac gtatatatat caagctaaag cattagaaaa ggcaggttat
      901 agtactgcga aggatgaaaa tggcaatagt atatatgcag ctcgtctaat agaattaatt
      961 caacagtata atcttcaatt aatagacagt gaaattcaaa gtgaaatgta actttgatta
     1021 aatttttatt gataaggcta tgatttttaa aatcatagcc ttattttgac atgcttttac
     1081 ttgtaattat taaatcgata tcattatctt tcaaaattta ctttatactg taaatcaaat
     1141 tatttaagtt aaaacttgga attatttata aaaggattat ttactttatc taaattctgc
     1201 acttaaagaa catcttttcc cttaagactg accgttagtc tata
//
LOCUS       DAEMGG010000263         1240 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEMGG010000263 DAEMGG010000000
VERSION     DAEMGG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.268"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            361..1041
                     /locus_tag="KPK24_003813"
     CDS             361..1041
                     /locus_tag="KPK24_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2301419.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKVRSITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          550 a           76 c          187 g          427 t
ORIGIN      
        1 ttatttttat aatagaaatt ttaatttata tctatattaa attgttaaag atataattat
       61 gagaataaaa aatatagcct tgaattatat aattgtatat gcttatcttt atacagtaaa
      121 taaaaaatca aatgaaatac caaataaagt gtatatctta aaacaattta ctagagatac
      181 aaggatgcta tgtgaatttt gaagtgtgca gtttagataa taaatatata taaataataa
      241 agaaaatgga tttatagtat ttaaaatttc ttataagggt aaaaaatgct attagaaaat
      301 gtactaaaaa tatttttcca attctttata gaaaaataat gatatttagg agagattgta
      361 atgcaaattg atgtattgat aattgatgga gataagaata gttctcaaga gctgaaaaat
      421 tttttagaag gaaaggggat aaatgtctat ttagcatata attgtaaaga tgctattata
      481 aaaatattta gcaagaaatt tgacttaata tttttagaga taattttagt tgatggtgat
      541 ggttgggatt tttgtaaaaa agttagaagt ataactactt gcccaattat tcatataact
      601 tatataaatg aaaatcaaag tattttaaat gctttaaatt ctggaggaga tgattattta
      661 ataaagcctt taaatttaga aatattatat gcgaaagtga aagctatttt aagaagaaga
      721 gatttatttt taaataataa taaaaataat ataaaactag aagattataa tagagctagt
      781 ggagtaataa agttagaaaa taaaatgata aagttaactc caacagaaaa tagattatta
      841 aattacttta tagagaatgc tgaaaagact ctaactatta aggagatata tgaacatgta
      901 tggatgaatg agtatttaga tgataattat agtgtagtgg ttgctgtgag tggattaaga
      961 aagaaaatag agaaagatta taaaaatcct caaaaaatta taacgataag agaagtaggg
     1021 tattacttta acaagatata aagctttata aacagaatga atgataaggc atagataata
     1081 tgttagtata ttacaagagt ttttgttcta acgatatatt tgtaaaatta gatttaattt
     1141 acttaattaa attatagcag tagaataaat ataaaaaatg tctgaaatat tatagacatc
     1201 agtaaaatat ataaaactat aatataagtc gtaataataa
//
LOCUS       DAEMGG010000264         1177 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEMGG010000264 DAEMGG010000000
VERSION     DAEMGG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.269"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(311..1057)
                     /gene="cdtR"
                     /locus_tag="KPK24_003814"
     CDS             complement(311..1057)
                     /gene="cdtR"
                     /locus_tag="KPK24_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBG2301420.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          441 a          140 c          122 g          474 t
ORIGIN      
        1 tttatataaa tttcataaaa tatgtaggta tatatcataa aaatatgtat ttatatgata
       61 tgtcagtaca aaataacaac tgaatatttt aaagagacta tttcgtactt ttatgattta
      121 atgagctatc ttatcgaatc ataatctagg aacaattgtt ataatatcta atagtagtta
      181 tatatgatat agtcagtatg tgttttttca caatatgatt attatataga ggattcttag
      241 ggattactct ttttgtataa aaaagagatg gttcaaattt ttatcttgag acatctcctt
      301 tttctattta ttatgtttta ataatattct ttaaaatatt tctatatgta ttagatatac
      361 gacatatatg gccattactc attttaatat tatttttctt taggtcgaga gaaacaatat
      421 gatatttatt aactacaaaa gatttatgta cacggataaa gcatgcatct aaatctggta
      481 aagactttaa acttttatag aattcaaata ttctagaagt agtcactaat tttattttat
      541 gtggaatagt gcttgtttca aaatatataa cttcatcaaa tgaaattttc catagtgaga
      601 aatctgaata gatagtaaaa aaattagagc gataattttt ttctttaatg tatcttttgt
      661 gagcaatttt aatacaagct tttattttac tttcagcaat ttccatatca tgttttagta
      721 taaagtccat aacttctaac ctataatcga agatccctaa aatcatttct atatgatttg
      781 taagaaatat gattggacta atataatctg attctctaat tttttgtgct atccataatc
      841 catcacataa ttcattactt atcttatatt ttgcgtctac aactataaaa tatattctta
      901 ctttattttt ttcagtaagc tcttttaata acaaatttgc attatgatat aatcttacaa
      961 catcattatc aaaaccttct tttattaaaa tattatttat tttttctttt agtttcattc
     1021 caaaacaaac atcattatca aagattaata tatccactaa ataccctcct ataaaaaatt
     1081 caaaagtaaa aaaacaaaag tattttatag aaaatacata aaataacact aggagataaa
     1141 taagattaag gatagttaga attaaggtat atttatg
//
LOCUS       DAEMGG010000265         1133 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEMGG010000265 DAEMGG010000000
VERSION     DAEMGG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.270"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>1133
                     /locus_tag="KPK24_003815"
     rRNA            <1..>1133
                     /locus_tag="KPK24_003815"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          299 a          250 c          342 g          242 t
ORIGIN      
        1 ggatgtgatg gggagcgaaa tttagtagcg aagtagctga tttcacactg tcaagaaaag
       61 tctctatcga ggttaaaggt acccgtaccg caaaccgaca caggtgggtg aggagagtat
      121 cctaaggcca gcgagagaac tgttgttaag gaactcggca aaatgacccc gtaacttagg
      181 gataaggggt gccaccatca ggtggccgca gagaataggc ccaagcgact gtttaccaaa
      241 aacataggtt tctgctaagt cgcaagacga tgtataggag ctgacgcctg cccggtgctg
      301 gaaggttaag gggatctgtt agagcaatcg aagcagtgaa cttaagcccc agtaaacggc
      361 ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt tccgacccgc
      421 acgaaaggcg taacgatttg ggcactgtct caacaacaga ctcggtgaaa ttgtaattcc
      481 ggtgaagatg ccggatacct gcgacaggac ggaaagaccc catggagctt tactgtagct
      541 tgacattggg tcttggtact acatgtacag gataggtggg aggctttgaa accaggacgc
      601 cagttttggc ggagccatcc ttgggatacc acccttgtag tactgggact ctaaccatag
      661 gccatgaatc tggtcttggg acactgtcag gtgggcagtt tgactggggc ggtcgcctcc
      721 caaaaggtaa cggaggcgct caaaggttct ctcagtacgg tcggaaatcg tacgtagagt
      781 gtaaaggcaa aagagagctt gattgcaaga catacaggtc gagcaaggat gaaaatcgga
      841 cttagtgatc cggtggttct gcgtggaagg gccatcgctc aacggataaa agctaccctg
      901 gggataacag gcttatctcc cccaagagtc cacatcgacg gggaggtttg gcacctcgat
      961 gtcggctcat cacatcctgg ggctgtagta ggtcccaagg gttgggctgt tcgcccatta
     1021 aagtggtacg cgagctgggt tcagaacgtc gtgagacagt tcggtcccta tccgtcgcag
     1081 gcgtaggaaa tttgagaaga cctgtcctta gtacgagagg accgggatgg acg
//
LOCUS       DAEMGG010000266         1109 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEMGG010000266 DAEMGG010000000
VERSION     DAEMGG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.271"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(234..1103)
                     /locus_tag="KPK24_003816"
     CDS             complement(234..1103)
                     /locus_tag="KPK24_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG2301421.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          415 a          175 c          105 g          414 t
ORIGIN      
        1 tctaatcaat cctaaataat aatctaaaat acatcaaaaa ataaactaaa aaatatcgaa
       61 aaataattta aaagacatca aaactaaatc ttctaatcag caattaaaag tctattttct
      121 tagtttaaaa aataaagata gattttttca aaacactgga taaataggta aaaaaataaa
      181 cagttagtaa aataaatttt tactaactgt ttatttttat tgtttaatat gtcctataaa
      241 tcctctaagt aatactttcc ttgcaaaaat tctgattcta aacctagatt tttaaaataa
      301 tcgaccatgc ccatacacgc aaatttttct gtagaataat gagtagcacc tataacatta
      361 attaaattat cttttgctat ttgatggaac tctaatactg gttcaaaatg ttttaaaggc
      421 ttagtaaacc cagtaatata tagatttata ccaagttcag caacttctct tgctacaaat
      481 ggataacttc ctccccctgc tgcaatagca actaaaccat cttttaatac agaatctcca
      541 taatcataca atttcacatc atgaccaaca gattttttaa ttaattcctt aatctcttct
      601 attgatttta gtgatgtttt acatataact cctacttcaa ttttatcgtc atatttacaa
      661 aatggtttga ctatttctaa acccatagct tttgcaaaac taacagaagt tgaatagtca
      721 ctatagttgt ctagtggaga atgtaataca taaaaagaaa ttctacgatt tttcatctct
      781 tccatataag atagtggtat gtcgtgaaat ggaaaacctt catctgatgc tatgtatccc
      841 attgcatgat gtgaaaaaat taaaatatct ttttcatctt tatctataac ttgcttaatt
      901 atttctttat caggaaaagt agttgtgtat actttattaa tattttgagc attatctaat
      961 actaatccta tgtatttttt tctaaattct ggagtaataa aatcattttc aaaattcata
     1021 aaactccaat catcattaat atttttaata ttaaattcta aatctaattt tttataaagt
     1081 tcaattgcct taataataac cactccttt
//
LOCUS       DAEMGG010000267         1019 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEMGG010000267 DAEMGG010000000
VERSION     DAEMGG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.272"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            58..813
                     /locus_tag="KPK24_003817"
     CDS             58..813
                     /locus_tag="KPK24_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HBG2301422.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT          433 a           69 c          165 g          352 t
ORIGIN      
        1 attacagttt atatattata ataaaataaa ttgttaattt taaaagaggt gaattttatg
       61 gcacatatat gctttgagga tatttgttat ttgttattaa aagaagcaat gaagttaaag
      121 ttaataccac ataataaaat tgtattattt gaagatgatt tatcaatagg taatattttt
      181 gatgtaaata actacgaatt aagagaaaat atttttaaga ttagaaatgt ttttgaaaat
      241 aatccgaaac tattattcaa gccagatttg ttagaagata ggaaattatc aaaatgtaga
      301 aatataataa tatggtgtgc aaataaccca aaagatttgt gtggagtata ttatgtagtg
      361 agtaagtttc aagataaaaa tataaagatt gcattagtag atgaaaaaaa gacagaagat
      421 gctctaatta gatattcatt taccagtgaa atgtctccag aagattttcc attcttttta
      481 gataagacac tagaaattgg ctttaatgaa aaactagaat ttaaaaataa atgggatgtg
      541 ttagttaaag aaaattctat ttttagagca ttagtaggta acgaaattaa aagtgttgaa
      601 gaaacttatt ttgataaata tatattaaga aatatcagtg acaaatggca acctttaatt
      661 gaagtggttg caaactgtat gtttgaagat gaaatgcgtg taaaagattg gtttatactt
      721 tggagattag agaagatggc aagtttaaaa atagtaaaaa taaaaggaag taaaggggat
      781 ttttattctg ataaggagat aagaaaagtt taaaaattga atttataata aattgtatta
      841 aataatgggt ataactatga agaaaaatat cttaacagta ttaatattat aattagcaat
      901 aagtataata tttattctat aatttggaaa tataataact aataccttac gaataagtgt
      961 gctaaataaa aaagaaatac ttatttcagg gatattagta tatattttaa ctacatttt
//
LOCUS       DAEMGG010000268          989 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEMGG010000268 DAEMGG010000000
VERSION     DAEMGG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.273"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(197..703)
                     /locus_tag="KPK24_003818"
     CDS             complement(197..703)
                     /locus_tag="KPK24_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG2301423.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          347 a          148 c           94 g          400 t
ORIGIN      
        1 attcaaataa atctctactt atataagtgc ttatataaat aaaaagatga actatagtaa
       61 atatagttca tcttttttat aatagtattt atttaaaatt aatatttatt tagttagtag
      121 catctactta gatatcagat aagaaagcac agtcatttac ttataataag ttcttttcat
      181 atatttttta ttataattat tcttttacaa gaacttttat aatttctgaa atgtacatag
      241 tatgatagtc tttctctgga taccattttt tatcattttc tttagcatca aagttttctg
      301 gtggcatatt accatgatat agctttttac atactataat catattagct tcttcaaaag
      361 ctaccgtatc atccacaaaa tatggtgtaa gtcctgcttt ttctatttta tttatatctc
      421 taccagaaat agtgccacaa atatttaaag cttctctata cgtttcatca aagaaggcaa
      481 ttgtaaatgt atcattacta tctacaaatt cttttgtata tctctgtggt cttatgtaag
      541 ttgtaacaac atttttaccc caatagactc caacaccacc ccagctagca gtcattgtgt
      601 taaacttttc tgaatcacca gctgttataa gtaaccattc tttgccaatc ttagtaaatg
      661 gattaaactg taattcctca atttttactt ctctaaaacc catgacttca tcctaccctt
      721 cattttttaa ttatataaat taattgttaa aaatttgaat atatgtactc ttataatact
      781 gacttttgat tgtcctacat aattcatgta aattataata caacaaatca attactttcg
      841 caataagtag ttttcatatt ttataagtgt aaaatataga taaaatatat tgattataaa
      901 gtatataata tatttttaaa cctaaagcaa tatacaataa aaaaatgtag aaagtaagta
      961 tctctctaca ttttttattg tatattatt
//
LOCUS       DAEMGG010000269          987 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEMGG010000269 DAEMGG010000000
VERSION     DAEMGG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.274"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(22..95)
                     /locus_tag="KPK24_003819"
     tRNA            complement(22..95)
                     /locus_tag="KPK24_003819"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61..63),aa:Cys,seq:gca)
     gene            complement(103..178)
                     /locus_tag="KPK24_003820"
     tRNA            complement(103..178)
                     /locus_tag="KPK24_003820"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(143..145),aa:Lys,seq:ttt)
     gene            complement(187..263)
                     /locus_tag="KPK24_003821"
     tRNA            complement(187..263)
                     /locus_tag="KPK24_003821"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:His,seq:gtg)
     gene            complement(271..347)
                     /locus_tag="KPK24_003822"
     tRNA            complement(271..347)
                     /locus_tag="KPK24_003822"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(311..313),aa:Pro,seq:tgg)
     gene            complement(377..453)
                     /locus_tag="KPK24_003823"
     tRNA            complement(377..453)
                     /locus_tag="KPK24_003823"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(417..419),aa:Ile,seq:cat)
     gene            complement(468..541)
                     /locus_tag="KPK24_003824"
     tRNA            complement(468..541)
                     /locus_tag="KPK24_003824"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(505..507),aa:Met,seq:cat)
     gene            complement(548..623)
                     /locus_tag="KPK24_003825"
     tRNA            complement(548..623)
                     /locus_tag="KPK24_003825"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(588..590),aa:Phe,seq:gaa)
     gene            complement(627..715)
                     /locus_tag="KPK24_003826"
     tRNA            complement(627..715)
                     /locus_tag="KPK24_003826"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(679..681),aa:Ser,seq:tga)
     gene            complement(805..880)
                     /locus_tag="KPK24_003827"
     tRNA            complement(805..880)
                     /locus_tag="KPK24_003827"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(845..847),aa:Gln,seq:ttg)
     gene            complement(888..964)
                     /locus_tag="KPK24_003828"
     tRNA            complement(888..964)
                     /locus_tag="KPK24_003828"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(928..930),aa:Arg,seq:tct)
BASE COUNT          271 a          257 c          216 g          243 t
ORIGIN      
        1 ttgagctacc ttggaatatt ttggaggcga cacccagatt cgaactgggg atcaaggagt
       61 tgcagtccac tgccttacca cttggctatg tcgccacctt tttggtgatc tatccgcgac
      121 tcgaacgcgg gacaccctga ttaaaagtca ggtgctctac cgactgagct aatagaccat
      181 ataatttggg gtggataatg agagtcgaac tcacgacctc cagagccaca atctggcgct
      241 ctaaccaact gagctatacc caccttatat tggtcgaggt gaagggactc gaacccccgg
      301 ccccatggtc ccaaaccatg tgcgctacca aactgcgcta cacctcgaat ttatatttat
      361 attaattaat ttaatgtggt gggccatcag ggactcgaac cccagaccta ccggttatga
      421 gccgggcgct ctaaccaact gagctaatgg cccacattaa attttagtag cggtaatagg
      481 agtcgaacct atgacctttc gggtatgaac cgaacgctct agccaactaa gctataccgc
      541 catattttgg tgcccagagg cggaatcgaa ccaccgacac ggggattttc agtcccctgc
      601 tctaccgact gagctatctg ggcatttggc ggagagggtg ggattcgaac ccacggcccc
      661 tttcgaagtc actggttttc aagaccagct ccttaaacca ctcggacacc tctccacatt
      721 cggatactta aacttattct aaaattgaac ctaactatat ttaaaataaa aataattcaa
      781 atatagctta gtaagtacat catttggctg gggatgcagg actcgaacct acgcatacat
      841 gagtcaaagt catgtgcctt accgacttgg ctaatcccca atatatgtgg tgagcgcaca
      901 gggattcgaa ccccggacac acgccttaga agggcgttgc tctatccagc tgagctatgc
      961 acccacaata tatatatgta tatcaaa
//
LOCUS       DAEMGG010000270          954 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEMGG010000270 DAEMGG010000000
VERSION     DAEMGG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.276"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            33..950
                     /locus_tag="KPK24_003829"
     CDS             33..950
                     /locus_tag="KPK24_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301424.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
BASE COUNT          335 a          117 c          163 g          339 t
ORIGIN      
        1 atttaaatta aaatataaaa ggagtgtatt tagtggaaaa taataaatgt agagaggact
       61 ttagatttac acaagaatat gaggaagatt atccaaatac aaatgaaaga tactatgaaa
      121 attatcaagt agctgataga tactataatt atccaaataa atataaagaa cctaaaataa
      181 aacaatgttg ttgtaaaaaa agtatgagag aagcattaga acttctaaga tatgatgctc
      241 taagaccttt tgtaaacttt aatcaatttg cttttatctc agatttcttt atagtaggtg
      301 ctaatttggt aggtatagat ctttcagctc ctccaaaaga taatttatct ggacttgatg
      361 gtacttttga aagattttct gcttgtaact gtgatttaat agatatagct ggtagagtat
      421 cttatcctat tccagtccct ttaactcttg agggattaat taatactata ggaactatac
      481 caggagtagc tgaattaatt gcacttattg atgcagttat tcctcctacg atagaccttg
      541 gggctatatt agatgcaata cttgctgcta taattgattt tatacttgct gcatctactc
      601 cattagcaaa cgtagattta gcatcattgt gtaatcttaa agctgttgca tttgatatta
      661 cacctgcaga ttatgaagat ttcatagcat ctttaggtta ctatcttgat aaaaaacatt
      721 acaaagaatg taattgtaac tgcgattgtg atgattgctg ttgtaataaa ggtatcctag
      781 ataatcttta tatgtcaaat ataaataatc aagttactgt agtagctggt agtttggttc
      841 taactggtgt tgaagttcta ggtaagaaaa atgatgttat agtacttgga aattctaatg
      901 attcaagaat atactttgta tgtgtagatt ctatagatta tattgcataa gaat
//
LOCUS       DAEMGG010000271          868 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEMGG010000271 DAEMGG010000000
VERSION     DAEMGG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.277"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..676
                     /locus_tag="KPK24_003830"
     CDS             134..676
                     /locus_tag="KPK24_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2301425.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          365 a           72 c          147 g          284 t
ORIGIN      
        1 gacaaatatg ttaaaaaaat gataatcacg gagaactttt accttcttca attaatatat
       61 tattattagt aataacttta aaattatatt actattatat aaaaattggc ttaaaagaag
      121 gtgaaaaaag taaatgaaaa tatgcataac agtaggtcat agtatactaa aaggtggtaa
      181 aagtacaggt gtaaatggta tagttgatga atacaattat aataaaaaat tagctcctat
      241 gttagctgag atgcttataa gtcaaggtaa tacagtagat gtaataattt gtccagaaag
      301 gcactttatg tcagaaagag aagaattttt ctatagagtt ccaaaagtaa acagtggtaa
      361 atacgattta ttagtagagc tacatttaaa taaagctgat ggaacacaat gtggtacaga
      421 agtgctttac tatggcactg aaggattaga atatgctaaa agagtatcta atagacttgg
      481 agaattattt gaaaatagag gagctaaaaa aagagaaaat ttatatatat tgagaaatac
      541 aaatccagtt gcaatacaga ttgaaagttt tttctgtgat aatgtgaatg attgtaacaa
      601 agcaaatgaa tcaggttatg attatatagc acgattgatt acagaaggta tattgaataa
      661 agatataaat atgtgagatt atggtgattt aggtattgat taataccttt taatattaga
      721 tataaataaa aatacaatgt attagtataa tttaaacttt caaaagatag attagtttga
      781 ttattgacaa gagatacctc cttgaaaata ttgatttatc agtatattta aggaggtatt
      841 taaatataaa aatttaatta atttgcta
//
LOCUS       DAEMGG010000272          853 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEMGG010000272 DAEMGG010000000
VERSION     DAEMGG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.278"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>853
                     /locus_tag="KPK24_003831"
     CDS             <1..>853
                     /locus_tag="KPK24_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2301426.1"
                     /translation="QFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSISLLVSQN
                     IINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIETMMLEK
                     SLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVVLSQFQL
                     WLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYKEIMVFR
                     IGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQGILKKI
                     LIGDATTY"
BASE COUNT          326 a           79 c          121 g          327 t
ORIGIN      
        1 caatttttta aaggtgtaag aaattgtttt aatatgattt atctacatga taaaagatat
       61 ttatttatct ctatgtttat ggcaattttt caggggattg ttccatctat ttctttgtta
      121 gtaagtcaaa atatcattaa taatatgcaa acattttcga aaagtgtaaa agaaatatta
      181 gttttgttaa ttatgtattt aatcataaat ataattcctt caatttttca aagtttttat
      241 ggatattata gaataaagtt taataaaaat tttgaaaaac atattgaaac aatgatgtta
      301 gagaaatctt taactttgag tttaaaagat tttgaggatg ataatactta tgacatgata
      361 aatagagctc aatctcaaaa aggatttaat attactatac tatctgaaaa ccttatttca
      421 ataataagag aatcagtatc tacactttct cttattgttg tattatcaca atttcaactt
      481 tggctaataa tagttgttat tgttattcct gtattgaaat cattatattc cattaaatta
      541 ggaaaggaac aatatgatgt tagtgtaaac agaacttgga aagaaagaaa ggcctggtac
      601 atagattatt tgttgtcaaa aggttatgct tacaaagaga ttatggtatt tagaattggt
      661 tctaaactta ttaatcatta taaaaaatta aaagaggata ttatatctca agatatgaat
      721 atagctaaaa agaattttat tgctacgcta ttatgtacag ttttagattc aattgtatct
      781 gcaggaatta taggatttat aataattcaa ggtattttaa agaagatttt aataggagat
      841 gcgactacat ata
//
LOCUS       DAEMGG010000273          798 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEMGG010000273 DAEMGG010000000
VERSION     DAEMGG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.279"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            73..261
                     /locus_tag="KPK24_003832"
     CDS             73..261
                     /locus_tag="KPK24_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301427.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
BASE COUNT          308 a           69 c           91 g          330 t
ORIGIN      
        1 tatatataca taggtatata ctaaatatgt taaacttaat tatggaaaat ataaaatgaa
       61 aggaataaaa atatgactaa gactatatta tgtgattact gtaataaagg aataaataaa
      121 gatgataata agtatattac ttttcataag aaaagtcata tgaaaactaa catttgtatt
      181 aattgtgcat taaatttgat agataaagat aaattaaatg aaaatattat aaataatcaa
      241 catgattatt caaagaaatg aaaaagcact ctccataatg aagaatgctc tatataataa
      301 tgtttgactt agtaaagatg tgttggggtt acatatttac ttttttatta tatcatataa
      361 ctttgtgtat gaaaaagaat ttaaatcaat tttaaggtgt gttgagtaat attcttgata
      421 gtttatatgt tgatgaattt caaaaaaata agcactctta taaaaagagt acttttggta
      481 tatattcaag catttatcta atacaattat agcatgtatt atgttttagt atgataattt
      541 tcgttcgttt tattattaca acttctacta tagttttcat actttaacat caactaaatg
      601 aatttaatta agattactag ttaatcgttt tttggttctt tcatattctg aaatctaaaa
      661 ttttattatt tgtttattgt tattatttaa cacatgttgg tatttcaacg atttatctta
      721 ttgttaatat tcttattgct tccaaagtat atctaattat ttttaagata atttactaat
      781 ttttattttt tcaaacat
//
LOCUS       DAEMGG010000274          776 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEMGG010000274 DAEMGG010000000
VERSION     DAEMGG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.280"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            12..473
                     /locus_tag="KPK24_003833"
     CDS             12..473
                     /locus_tag="KPK24_003833"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG2301428.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLD"
BASE COUNT          295 a           90 c          117 g          274 t
ORIGIN      
        1 aggaagtaaa aatgatatat gaaggaaaac ttattggaaa agatttaaaa ataggtataa
       61 taaattctcg ttttaatgaa tttataacat caaaactttt atcaggagct gaagactgtc
      121 ttttaagaca tgacgtttct ccagaaaata tagaaatagt ttgggtacct ggagcttttg
      181 agattcctct tgttgctcaa aaaatggcta aaagtggtaa gtatgatgct ataatctgtt
      241 taggctgtgt tataagaggg gctacttctc attatgatta tgtatgtagt gaagtctcta
      301 aagggatagc caaagtatct ttagacagtg aattacctgt tatatttgga atcgtaacta
      361 cagaaaatat tgaacaagct attgaaagag caggtacaaa agctggaaat aaaggttatg
      421 attgtgctat gaatgcgcta gaaatggcaa atttatttaa gtctctagat taaataatat
      481 cattaataaa tattaaaaat tagtgtaaag gatacaaaat attaagtacc aaagggagaa
      541 actatctttc ttctttttgg tacttaatat ttttaattta atcatatgta cgttcttatc
      601 aaacaaattg catatccttc taaaattata cgttatccat caaatctact tttagaagct
      661 taaaataatt acaaatataa ccctaaacat aaaatatcat aaaaacatat atattactat
      721 tttagctatt gtttttattt atagcatata ttactagtat attaattatc tatttt
//
LOCUS       DAEMGG010000275          756 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEMGG010000275 DAEMGG010000000
VERSION     DAEMGG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.281"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(53..>756)
                     /locus_tag="KPK24_003834"
     CDS             complement(53..>756)
                     /locus_tag="KPK24_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2301429.1"
                     /translation="IICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLLKKYP
                     NNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKP
                     LKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGI
                     YTFKSSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRYKIK
                     LLREKLNSCLVDVII"
BASE COUNT          267 a          106 c           55 g          328 t
ORIGIN      
        1 ttttacaata gatatattct attagaatag aatataaagg caaaaattta cctcaaatta
       61 ttacatctac caaacaacta tttaactttt ctctcaataa ttttatctta taacgactta
      121 caattattga attttgattt ataaatacaa tattattttc tatatctagc atatttacct
      181 tcattaaatt tataaggtag cttttatgac atctaaaaaa attatgttct tctagatttg
      241 attctatttc tttcatacta ctcttaaaag tatatattcc tttttcagta tgtatagtta
      301 aatcattttt taaaacttct aaatataaaa tatcatctac ctttatgaaa tatatcttgc
      361 atttacattt tatagataaa atagcttcat ttttgtagca ttcttttaaa tatgatgata
      421 ttgcttcttt aaatagagag tattttaagg gcttctctaa atatctataa actcctatct
      481 cataaccttt ctttatgtat ttatttgatg aggatacaaa tataattctt acactttcat
      541 caaatttttt gatttctttt gaaacattaa atccattttc atcatttaag tctatatcca
      601 taaatatgat atcaacatta ttaggatact tttttaataa atcgcctcca ttttcaaact
      661 ctttgatatt gtattcaaaa ttgtaattat aaaaaaaggt atttatataa tcatttagaa
      721 ttaccctctc tgatttttta ccttcgcaaa taataa
//
LOCUS       DAEMGG010000276          716 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEMGG010000276 DAEMGG010000000
VERSION     DAEMGG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.282"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..634)
                     /locus_tag="KPK24_003835"
     rRNA            complement(<1..634)
                     /locus_tag="KPK24_003835"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          173 a          182 c          127 g          234 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccagttaa aaaggtttga tagattagaa
      661 gctatcaact tcgataattt taaaaagtta tcagctttat atattacaaa ttagat
//
LOCUS       DAEMGG010000277          713 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEMGG010000277 DAEMGG010000000
VERSION     DAEMGG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.283"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..372
                     /locus_tag="KPK24_003836"
     CDS             <1..372
                     /locus_tag="KPK24_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2301430.1"
                     /translation="DFLQKLSTKLIKENDIICIEDLQVKNMIKNHKLSRLISDVSWSE
                     FIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDR
                     DINASINILKEGLRLITIQNK"
BASE COUNT          299 a           78 c          103 g          233 t
ORIGIN      
        1 gattttctac aaaaactatc tactaaatta ataaaagaga atgatattat ttgcatagaa
       61 gatttacaag taaagaatat gattaaaaat cataaactat ctcgcttaat ttcagatgta
      121 tcatggtcag aatttattcg tcaattagaa tacaaggcta attggtatgg tagacaaatt
      181 gtaaaggtag gtaaattctt tgcaagttca caaatatgca ataaatgtgg gtataaaaac
      241 gaggaagtga aggacttaaa tataagagaa tggatttgtc ctagttgtaa tgaaactcac
      301 gatagagata taaatgcaag tataaatata ttaaaagaag gattaagact aataacaatt
      361 caaaataaat aacttatgta agaaccgtag gaactacggg gattgcctgt ggagaattag
      421 taagacatat tttagtatgc aaaattctat gaagcaggaa ccctgtgact ttagtcatgg
      481 gaggttcagt ttagcttaaa acttacttat ctatttcagt ttctattaga attattatta
      541 gactcattaa aataaaagca ctaatataac taataatata tgttaaattc attaactcta
      601 cataaagttt tacaatctaa atgaaataca ataatctgca taaaattcaa tagcttatat
      661 aaaatttaat aatttatata aacattatca atctatataa aatttagtaa ttt
//
LOCUS       DAEMGG010000278          657 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEMGG010000278 DAEMGG010000000
VERSION     DAEMGG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.284"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          279 a           45 c           88 g          245 t
ORIGIN      
        1 taaatatatg catatattta atcctgttta ttttcatata atatttcaag cttgtataat
       61 gtaacaatta aaccaaaaga ctcattgtat atttgaaatt aagtttttta tagtttatag
      121 gtgttttatg tggattacga atgatatagt attgatacat tatttaatag attcttatgc
      181 caagaaacaa ttaatttata gaaattctta attgttacaa tataaatatc tagataagtt
      241 ataagatact gataaataat ttataaagtt tattaatcta atattgtgtg tgaatattaa
      301 gtggtcaagt agtctaaaaa atgaaaaaga tttaaaaaag tgttttaact aaacacataa
      361 gtgttaaatt catgtataag tataggttag atacataaga aataattatg taatatctat
      421 ctttgtaaaa aaatattaaa ttttgtaatt tgtggataaa tatgtttata atgtggataa
      481 tatttaatgt aataagttaa agcaatatga atcaactgtt tataatgtgg ataatattat
      541 aaatgtatga ataattgcag tatttaaaaa caaaaaaagt taaaaaagtt gttgactcta
      601 aaagaaaaag ttggtatagt aatacttgtc ctcaagaaaa gggcaaacaa ataaagc
//
LOCUS       DAEMGG010000279          611 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEMGG010000279 DAEMGG010000000
VERSION     DAEMGG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.285"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>611
                     /locus_tag="KPK24_003837"
     rRNA            <1..>611
                     /locus_tag="KPK24_003837"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          126 c          179 g          134 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg c
//
LOCUS       DAEMGG010000280          601 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEMGG010000280 DAEMGG010000000
VERSION     DAEMGG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.286"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          266 a           56 c           61 g          218 t
ORIGIN      
        1 aattagactt ctctcttatt caagattttt ctatataaaa tctaagaata aatagaaaac
       61 taacatctaa ccttttattt tttattttcg atctatttac ttgaatttta acataaatct
      121 atcttttagt atatattaat catgctgaat ttgtgatgac atgtatacaa aatatcgttt
      181 agtattttct aataaaaaat attactgaaa ttaaaaatct acaatagcat ttaacaagta
      241 aaaacatgtt tacacaaata tataatttaa ggtctttaaa atagagtatg tattattcta
      301 aaaattagtt atagttatca cataaaaata gaaaagaatt ttcaaaagat ttaaagaaca
      361 tacatacttc aattgcagaa gatgtaatgt taagtgaact agcattcttc gaggaaaaat
      421 agactcaaaa aattagaaaa tagaatcagg ttttagatga tttaagtatt tattttcaaa
      481 aaagaaattt gattctaagt taataaagaa atatttaaaa aaatcaaaaa attttcttaa
      541 ataagtaata ggttttgtaa aaatggaaaa atatcaaaat aagactatga tttttaaaac
      601 t
//
LOCUS       DAEMGG010000281          577 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEMGG010000281 DAEMGG010000000
VERSION     DAEMGG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.287"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>577
                     /locus_tag="KPK24_003838"
     rRNA            <1..>577
                     /locus_tag="KPK24_003838"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAEMGG010000282          522 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEMGG010000282 DAEMGG010000000
VERSION     DAEMGG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.288"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            243..>522
                     /gene="tcdR"
                     /locus_tag="KPK24_003839"
     CDS             243..>522
                     /gene="tcdR"
                     /locus_tag="KPK24_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG2301431.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKM"
BASE COUNT          214 a           41 c           59 g          208 t
ORIGIN      
        1 tttactttaa taaaatgatt tgtttttaca atactttatt aatataaagt ttattgctaa
       61 aatactttat ttattagaaa aagattacta attaattatt aaaattaatg tattcataat
      121 gcatattttc atataaaatt taatttattt gccgattata taattataat gactgattta
      181 attccaatgt tgtcaaaatt ttcaaataaa tcatcattat aaatataaga gaggatgatt
      241 ttatgcaaaa gtctttttat gaattaattg ttttagcaag aaataactca gtagatgatt
      301 tgcaagaaat tttatttatg tttaagccat tagtaaaaaa acttagtaga gttttacatt
      361 atgaagaggg agaaacagac ttaataatat tttttattga attaataaaa aatattaaat
      421 taagtagctt ttcagaaaaa agcgatgcta ttatagtcaa atatattcat aaatcattac
      481 tgaataagac ttttgagttg tctagaagat attctaaaat ga
//
LOCUS       DAEMGG010000283          405 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEMGG010000283 DAEMGG010000000
VERSION     DAEMGG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.290"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..215
                     /locus_tag="KPK24_003840"
     CDS             <1..215
                     /locus_tag="KPK24_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2301432.1"
                     /translation="IGSHPSFQLFHDLVTMFNISVDEYFYPAEKVAKSTARRQIETSL
                     DLLSDNELKIIQGTIDGILNSRENKK"
BASE COUNT          125 a           73 c           68 g          139 t
ORIGIN      
        1 atatcggttc acacccaagt tttcaattat tccatgattt agttaccatg ttcaatatat
       61 cggttgatga atatttctat ccagcagaga aagttgcaaa aagcacagct cgccgtcaga
      121 tagaaacatc acttgattta ttgagtgata acgaattaaa aattattcaa ggtacgattg
      181 acggtatctt gaacagcagg gagaataaga agtaatcagc ttcttgtttt ccccgttttc
      241 tttttagcca tatttcttca agacttattt gttgatctgc tttgcaggaa acaaataaga
      301 gccgacaatc tataagttac aataacctac aaattatcgg ctctgcgtct tagcgtctgg
      361 ctctttgata atagttactt ttccatgctt tatattgatt aaagt
//
LOCUS       DAEMGG010000284          384 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEMGG010000284 DAEMGG010000000
VERSION     DAEMGG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.291"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>384
                     /locus_tag="KPK24_003841"
     CDS             <1..>384
                     /locus_tag="KPK24_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301433.1"
                     /translation="LTLDTRNLIKKYSLTLNEDLIWEFKHGKYHTIKYFTHKFAVKES
                     VLALLFNIHKLCYAKIKYFESSFSKYEPYKYSYQNEFVKCEMYDMNFMFHKYSNIMID
                     LRNLQEIKTIEEFRLFCDYLESFED"
BASE COUNT          153 a           40 c           53 g          138 t
ORIGIN      
        1 tttaacacta gatacgcgta accttataaa aaaatattct cttactttaa atgaggattt
       61 aatttgggag tttaaacatg gtaaatacca cacgataaaa tactttacac ataagtttgc
      121 agttaaggag tctgttcttg cacttttgtt caacatacac aaattatgtt atgctaaaat
      181 aaaatatttt gagtcgagtt tttcaaaata cgaaccttat aaatatagtt atcaaaatga
      241 atttgttaaa tgtgaaatgt atgatatgaa ttttatgttt cataaatata gtaatataat
      301 gatagatttg agaaatttac aagaaattaa aaccatagaa gaatttagat tattttgtga
      361 ttacctagaa agctttgaag ataa
//
LOCUS       DAEMGG010000285          381 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEMGG010000285 DAEMGG010000000
VERSION     DAEMGG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.denovo.292"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>381)
                     /locus_tag="KPK24_003842"
     rRNA            complement(<1..>381)
                     /locus_tag="KPK24_003842"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           89 a          107 c           87 g           98 t
ORIGIN      
        1 tgtactaccc attgtagcac gtgtgtagcc ctaagcataa ggggcatgat gatttgacgt
       61 catccccacc ttcctccagg ttatccctgg cagtctctct agagtgccca acttaatgat
      121 ggcaactaaa gacaagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga
      181 gctgacgaca accatgcacc acctgtcacc aatgtccccg aagggaactc tccgattaag
      241 gagatgtcat tgggatgtca agcttaggta aggttcttcg cgttgcttcg aattaaacca
      301 catgctccgc tacttgtgcg ggtccccgtc aattcctttg agtttcactc ttgcgagcgt
      361 acttcccagg cggagtactt a
//
LOCUS       DAEMGG010000286        13997 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.guided.103, whole genome shotgun sequence.
ACCESSION   DAEMGG010000286 DAEMGG010000000
VERSION     DAEMGG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.guided.103"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            77..754
                     /locus_tag="KPK24_003843"
     CDS             77..754
                     /locus_tag="KPK24_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301434.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(1082..1849)
                     /locus_tag="KPK24_003844"
     CDS             complement(1082..1849)
                     /locus_tag="KPK24_003844"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG2301435.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(2521..3336)
                     /locus_tag="KPK24_003845"
     CDS             complement(2521..3336)
                     /locus_tag="KPK24_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBG2301436.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSSRAEMERL"
     gene            3428..4696
                     /locus_tag="KPK24_003846"
                     /pseudo
     CDS             3428..4696
                     /locus_tag="KPK24_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4795..5415
                     /locus_tag="KPK24_003847"
     CDS             4795..5415
                     /locus_tag="KPK24_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2301437.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            5565..5969
                     /locus_tag="KPK24_003848"
                     /pseudo
     CDS             5565..5969
                     /locus_tag="KPK24_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            6094..7020
                     /locus_tag="KPK24_003849"
     CDS             6094..7020
                     /locus_tag="KPK24_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2301438.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            7034..7483
                     /locus_tag="KPK24_003850"
     CDS             7034..7483
                     /locus_tag="KPK24_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2301439.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            7843..8250
                     /locus_tag="KPK24_003851"
     CDS             7843..8250
                     /locus_tag="KPK24_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2301440.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            8252..8482
                     /locus_tag="KPK24_003852"
     CDS             8252..8482
                     /locus_tag="KPK24_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2301441.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            8872..9075
                     /locus_tag="KPK24_003853"
     CDS             8872..9075
                     /locus_tag="KPK24_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2301442.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            9153..9506
                     /locus_tag="KPK24_003854"
     CDS             9153..9506
                     /locus_tag="KPK24_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG2301443.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            9578..10210
                     /locus_tag="KPK24_003855"
     CDS             9578..10210
                     /locus_tag="KPK24_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG2301444.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(10581..11450)
                     /locus_tag="KPK24_003856"
     CDS             complement(10581..11450)
                     /locus_tag="KPK24_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2301445.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(11547..12404)
                     /locus_tag="KPK24_003857"
     CDS             complement(11547..12404)
                     /locus_tag="KPK24_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2301446.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(12619..>13997)
                     /locus_tag="KPK24_003858"
     CDS             complement(12619..>13997)
                     /locus_tag="KPK24_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG2301447.1"
                     /translation="KDDFYISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKN
                     ILEESSINSKSDVLVVATKEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKE
                     IYSVLENLYEFLVDYKKVKSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVK
                     IALATAVAGFIMDYFHLRDGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIV
                     LFSIIKDSTLRSLIVLLAGYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRIS
                     YVALGAIIALIVNKFILPYDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKN
                     LLLIPSLIEDRLMLINAIYKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKI
                     LRDTNYISNYNVDKYDEGRSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNIT
                     KVSIKEAA"
BASE COUNT         4876 a         2139 c         1980 g         5002 t
ORIGIN      
        1 gtcttatagt gctaatgagt actattagac catgttctat ggccttattt taatataaaa
       61 cgattggaga taggctatga ttgtgattga aggtagcgat aaatttaaga ttgcaaaaga
      121 atatattgat gtagaatata ctctttttag caaagtaact tttaggtatg aaaagttgaa
      181 atttaaagat aatgctgaat tggaaaaaat taagatgttt aaatataaaa atggctacat
      241 ccctaataaa ttaaaccttt cttttggata tggattctct tcttataaaa agcaaataat
      301 tagagaaact gtagatactt taagattgac agaaattttt tcaagcgaga acatagaaga
      361 tattaaattt ataaaagatg gtacaaaaaa attagaaatt agcatagaga aagttgtgaa
      421 atttaaacgt cgaaaaaaaa gaaattatgt ttgttgctat tgccctgata tgtatagaga
      481 cataaaactc gacaaagaat ctatcaataa gatatacaac agaaaaataa aaatagaaag
      541 agaagttaat atttttgaag atgaggatgt tataataaac aagaaagtat tgaagtttcc
      601 aaagtcttgg acaaagaata tgcaaaaata ttggttaagt gaaaataagt atcccataca
      661 ttctactgta attgatgatg atagatataa atgttgtaat gtacaatata caaaaaatag
      721 agtgataata ttatattaca tatataacca ttaatataca taattacaaa aaaataatga
      781 caaatataca taatacaaat atatcttcta ctttagataa aacagttatt atgaaaactt
      841 actatctata aataatagaa atgctagtat accatctata atctaatgtc tagtaggttt
      901 taatggcata ttttaaatat gccatagttg atttataaga ttttctatat cactttcaaa
      961 atacacacta aatagttgct tttcagatat aattgcccaa tttaaatttt actatctgaa
     1021 aagtttttta tatttaattt atatcttcaa aaataccgtc taatatttct atataattaa
     1081 tttaattaat actcttatgt aatagattaa gtactgaatt taactcttta aatgatattt
     1141 gccctggtgc tgaaacactt tttgcagctc caaaagtcaa tgcagaacca aatatttcac
     1201 cacacaatct acttataaca cccattcctg acattgacat tgttattatt ggtctatctg
     1261 cataaatttt aaacatttca tttgtagctt caagtagcac taacacatct ttttcatttt
     1321 gcggcataac agctattttg ggtaaatctg cgcccaattc ctgcattcta cataaacgag
     1381 atacaatctc ttcttttttt ggagttttat taaaatcatg attagatata attactttca
     1441 cttctttttt atgagcaaaa ttaactactt catctatgac ctcatctccc ataaataact
     1501 ctacatctat taaatctact aaacctgtat tagaaatttc tttattcaaa gtagtataat
     1561 aatctcttga tattaatttt tctccccctt ctactacact tctaaatgtg aataataaag
     1621 ggatatcatg tatataactt cttaattcat agagaacttc cttaacttct ttaatatttt
     1681 ctacattctc aaaaaaatca actcgccact ctataatatc caaacatgca tcttttaact
     1741 ctttagcctc ttttataata tcttttttat ttttcccaat aattggtaca caaattttag
     1801 gtctaccttc tccaatagta atatttttta cttgaacttt tcttttcata ttctcctcca
     1861 taatcagaaa ttctatagga ctttaaattc tattttctgc acaatttaat tatacattaa
     1921 aaatactata cattataaaa atactaattt aaaaataatt tttattctaa ttcttaaaac
     1981 aatcccacat attttagaaa aggtactcta taatatacaa acacttcttt tttacctaaa
     2041 tttattttaa catcctataa ttttcacaat attctttaaa ttttactcat ataaacaata
     2101 ttattctgga tgctaataga attaatctat aaatcatagt ataattatgc agaaataact
     2161 cgaatttcac tacttatgaa gagattgaat attaatgaat ctaaatcatt tgcattactt
     2221 aagagttttg actaaagttg aacaccatac aaaagtagct actcaattgt ttatacccca
     2281 accaagctta agtaatgtta tatagattct cgaaaaagaa tttggtatct ttttatttga
     2341 aaaacttata ttaaatttga ttatcttttc ataagttggt aaatgtcgat atatcctgta
     2401 tctacaagga gtatcggcat tttctttttg gaagatactt tgcataagtt ggcatttaac
     2461 cgtataagaa tgccatcaaa agtagtaaat aagtagtaaa ctctagtcat actactagga
     2521 ttataaccgt tccatttcag ctctcgaaga actaaatgaa ccatgtgcat aatagccaag
     2581 cgtcatagta atgtttttat gccccataat atattgcaat atattaggac tcattccttt
     2641 gttagccata tttgtacaga gtgtatgcct aaaggtatgt ggcgtaatct ttggtagctt
     2701 gtcctcatgg gtcttattat acttcttgac taacgctctt aacatgcttt gataatgagt
     2761 tgcttgtttt ggtaatccat cttgtttaag aaataggaag ttctggtaac catcaacaat
     2821 gattggttct gctttagcgt tcttacgccg ttccacgata cgttcaaatg attgataagc
     2881 tctttcagtc attggaattt ctcgcttacc ctgtgtggtc tttggaactt ctacataata
     2941 gccaatctta gtatcactca acaattgatg gtctaccata atggctttat ttttcatatc
     3001 caattctaaa gtcaaaccgc ataattcaga aatgcgtagt cctgtttcta agaggataac
     3061 aacatcatca taatatttac aataagtctt atcgctctcc ataaaggata ataagcccat
     3121 ttcctgttct ggtgttagag cgatttttgg ttctgttgta tcttccaaaa catcacttaa
     3181 ctgaaaatta aatggattct ttctaataca gtcgtcttga attgccatga aaaacgacgc
     3241 tttcagtgag cgcttataat tgtcaatgga tttataagca cacccatctt cactcattcg
     3301 gatagcccag cttttagcgt ctgataattt aaccatatca atgctttttg cacctagtgg
     3361 gtcattgata gtatctatat tttgatttat aatttataca tttttttaaa aacagacttt
     3421 taactgtaaa gaatctatgt ataaacttag ctatgttgtc cgtgtatcta caaaggatct
     3481 tgacgagcta aaaaggcgtt gtgatgaagt caaagacttt tacgacgact tatcactcaa
     3541 gctcgtgcgt ccctttgggg atatgttggg attgcatggc gaatttattc ctgtcagcaa
     3601 acgttacatc aatgattata ttcaatatgt cacttcggat tttttggctg gacttggttt
     3661 tggagcgaca caaacccttg gcgaacatga ggatatctat cttggctaca atctggacac
     3721 tggaaagaat gtctatctaa aacctgccct tgctagtcaa tgggtaaagg gttctattac
     3781 caatgtgtta gcgtctgcgt ttatcggttc attgggtggt ggtaaattat tcagtaacaa
     3841 tatgcttgtt tactatgcgg tactattcgg cggacaggcg gtcatagttg accctaaagc
     3901 agaacgcggc aaatggaaag aaacgctacc agaaatagcc catgagataa acattgtcaa
     3961 tttaaccagt aatgatgaaa acaaaggatt gcttgaccct tacgtcattt tgagccgtaa
     4021 aaatgattct gaaagtttag cgattgatat cctaacattc cttgctggta tctccagtcg
     4081 tgacagtgat aagttcccag tgcttagaaa agcaatccgc aatgtaaccc aaagtgaaca
     4141 gcgagggtta ttacttgtca ttgatgaatt acgaaaagaa gatacttcta tcagtaacag
     4201 catagccgac catatcgaaa gttttgtaga ctatgatttt gcacatctac tgtttggtga
     4261 cggaaatgtg aagcagtcta tcagcttgga gaaacagctg aatattattc aagttgctga
     4321 cctcgtattg ccagatgcca acactagctt tgaagaatat acaacaatag aacttcttag
     4381 tgttgctatg ttgattgtta ttagtacctt tgcattagac tttattcata ctgaccgctc
     4441 tagcttcaag atagttgacc ttgacgaagc atggtctttt ttgcaggtgg cacaaggtaa
     4501 aacgctatct atgaaactgg ttagagcatc gcaagattca caggacaaac gaattgaacg
     4561 tttgaagaat tatctgactg aaaacctaca acagttgaat actgaaatgg ttcgctcata
     4621 tattcctact agttctattg tcaacagctt tctcagtttg agcttacttt gcagaaaaat
     4681 gacaactgga aaataataat ggaatagttt ctatgtaaat atatttttac tatcttacct
     4741 aactaattga aagatgatat aatttaatca aagatttggt aaggagggat gaatatgcat
     4801 ttaattgatt ttatgaatga agatattaaa aagaagcttg ttaaaaaaac gtataaaaag
     4861 aaagagacta ttctttttgc tgagcaagaa aatgaatatg taattcttat gattgacggt
     4921 attgcagaag cattcatctt aaatgtgaaa ggaaacatct caactattca tctttataga
     4981 agtggtagtt tttttggaga aatggaacaa tttaatgatg ggaaaaaacc cgttgaaatt
     5041 gtcgctttca cagattgtaa tgtatataaa cttcatagag atgatttttt aaactggtta
     5101 aggggagatt ttgaagctac aaaatttctg attcgagaaa tatctagtaa attagttgta
     5161 aatgctgaat taattgaaga attatcatct ttaagtgtta aagaacgact tctgaggtgt
     5221 atatccttac actattatag aagtgaatta aatctgctta ccaaaaaaca gcttgctagc
     5281 gaagtaaacg ctccaattag aagcattaat cgtgctataa atgaatgtgt acaagaacat
     5341 ctaattgctt atgaaaacaa aagatttagt gtaattaatc agcgagaggt attaaagaat
     5401 cttcctacaa cataagtata atttcaaaaa agtattgaat aataattatt caatgctttt
     5461 ttatttcggg tcaagtgacc tatttttttt tattttttca ttatataata ctcctatgca
     5521 taattatgtt ggattgatag ctaataaaag aaagtgagag gtgttatact aaaaaagaag
     5581 catacttccc aactccagac ttacagtgga ttgaagatag attttggatt tgggttcatt
     5641 acgcttgcac taagattgga cgtagagaac tatttgaaac aattgatttt ttatcttttt
     5701 taagacaatc agtacttgcc ccattaatac agcttctctt aggtaaatta cctcgtggag
     5761 ttagaaaaat tgaattggac gcaccccaat atattgaagc attagaaaag acggttgcta
     5821 accatgaata taaaagctgt gttaatgcat tgcaggctac tatcaacctg tatcttgagt
     5881 taagagagga attatcacaa agtcctattg tccgaaaggt tgaagctgaa agggttgcat
     5941 tacagtattt tcaagattta atcaaataaa cccaaagtaa aaaaatattg aatagtaaat
     6001 attcagtgtt ttttcatttt tggtcaaatg acctagatta gttgttgtaa ccattctata
     6061 attattttga aagtaacagt atggaggata actatgagtg atattttaag tagaattaca
     6121 aataaaattg gggataagaa tatagttgat aagttatcag ctctatcaaa atcagatttg
     6181 aactcacttc tattagaagt gtttgataga caagcaaata ctttaactgc aactgatatt
     6241 ctaaagtcat accaactaaa tagatttact atccctagcg gtatagatcc agaagaaatt
     6301 catgctttgg agtcaaaatt attgagaaaa gcattcaata tggatatcaa gacgattatg
     6361 ctttctccct ctgctccatt aggtagctgt agcgtttttg gctccgtaga tcaatataat
     6421 gtagtaagtg ctctacgagg gacagaaaca cttgcagacc cctcaaatat gttggcaata
     6481 attattgctg ataaattaaa aagaaaagaa gaaaataata ccatacctat ccacatggct
     6541 actactgcta gggtaataag agcacaaaat tttgtaggta aaggtttata ttcacatttt
     6601 ggcttatatt gtatggtttc atctggtata gatacagggt cttacacttg tgaaaaaatg
     6661 ttgttagaaa agcacttaaa ctattataaa gatatattaa gtgaaataga aaatgttcgt
     6721 ttctctatta ttctacgcaa aagaaacgga tacaaagata atgacggatt tttcgacaga
     6781 atggttgaaa ctatcaaact tatttttcct aatatagagt tatcaattca taatgatgat
     6841 gttgataata attattataa aggtatcaat ttcaaattat cggtcaaaca aggaaatgaa
     6901 accgttgaaa tagttgacgg tggatttgtt gactggactt atcaaatgct tggaaataaa
     6961 aaagaacgct gtttgataag tggtattggc ttagaaagat ttcttgtagc tgtttcataa
     7021 aaggagaaga aaaatggaac ttatagagtg gaattatgct tatattaccg atttagttga
     7081 gtttgcgaat gataaagaaa ttgcaaagaa tcttcgtgac aacttccccc acccttatag
     7141 ggagcaaaac gctagagatt ttgttagttt ttgtttggca actccagatg ttaaacaact
     7201 tagctatgct attttttatc aagaaagggc tattggaagt atcagcctaa catttgaaga
     7261 agatacctct attaataaaa atgctgaatt aggttactgg cttgctagga actattgggg
     7321 caaagggata gcaacaaatg cagtaaaaga aatatgcaaa attgcctttg aaaaatacaa
     7381 ccttaacaaa attcatgcta atgtatattc ctacaataga gggtcaagta aagtattgga
     7441 aaaatgtggt tttaagattg aagaatactg tcacaattca taataaagaa tagaaacatg
     7501 attgattgcc atgtatatag tttaattcga ccgcaatagc atttattaga attaacctac
     7561 tttttaataa cagaataagt ttttcccctc tattaatttc ttatgcatct ttgtattttt
     7621 atgaaaagct agccaatgca tatttccata catcaatatg gtggtggctt acaaccaata
     7681 taaattaaat acgttttaaa gaatacacat ttttgcccta taggcagagt gtgtattttt
     7741 ttctcttttt tttgaaattt atcgaagatt taacgccctc ccaccgtgat aggagtaagc
     7801 aaggttattt aactccttgc gatggaaaga gggtgaaatc ttatggttga tgaaaagaca
     7861 attgaacatc aattcgacag tttctgtaaa acagttttgc gtaactatgc tagagatatt
     7921 tatgacgaaa ataaacgcag aaatgattat ttggtatctc tcgaatcttt gtcacttgca
     7981 gaattaagta aattgagtat tttagatgat tatgattcta actacatctg tatggtttct
     8041 tacgactaca acatacgaat tgaagatgta ttaatggcac aagcaatcgg aaaacttaca
     8101 aaaagaaaac aagatataat tctactttca ttctttctca acatgacaaa tgctgacata
     8161 gcaacattga tggatttagc agaaaatacc atacattatc acaaaacaaa tgcactaaaa
     8221 gagttgaaag aactcatgga ggaacattga aatgaaacct actaaagaag tattaccttt
     8281 tacaattatt ttacttgctg tacatggcga tgtaaatgca ataaacggaa tacttaaaca
     8341 ttttgaacac tatatcattc gtctttcaca gaaaacttta tttgatgaat acggaaatcc
     8401 ctatattcat gtagaagaag aaacaaagcg aatgctagga acaaaactaa tcactgctat
     8461 tttgagattt aatttgtctt aatctcgccc tctctccatg tatcaagcta tttggtacat
     8521 ggagaatcac taattatttg tctataaaag cccataagct attgtgattt tttatagaca
     8581 aatcaatgcc gactgttctt tgacaactga ataaagtaga tagatacgtt tgtgatacag
     8641 atagccaaca aatactatac gccatgacct taatcaagag agctactata tagtgatttg
     8701 tgaataacct ttggaacggt tgttttgccg taacctgtac cacagtataa tgatacacct
     8761 gtatgacacg gttctgccca taagaatggg attggttaaa ataccaatgg agcttaccaa
     8821 agccatctat ctacttctga actacgaaca tagaaaggag tgatacattc tatgaacaat
     8881 actgatgtac ctatttggga aaaatatacc ttgacgattg aagaagcgtc aaaatacttt
     8941 cgtattggag agaaaaaatt acgtaaactt gctgatgaaa accttgattc tggctgggtc
     9001 attatgaata gcaatcgcat tcaaataaag agaaaacaat ttgagaaaat cgttgataca
     9061 cttgacttta tctaatgaaa ttgagcctta tatgttataa tattcatatg acgtatcaag
     9121 ctcaattcgg ttattgagag gagcttctta tcatgtcaac tgaaaaaaga cgagataata
     9181 aagatagaat tttaagaact ggagagagcc agagaaaaga tggaagatac gcatataagt
     9241 atgtatattc atttggtaaa ccacaatttg tttactcttg gaaacttgta cctactgata
     9301 aacccccaaa gggaaaacgt gatgatatat cattaaggga taaggaaaaa gcaatacaaa
     9361 atgaccttga tgatagcatt gacactattg gtaagaaaat gacagtttgt cagttatacg
     9421 ctaaaaagaa tggactaaga aagaatatcc gacttaacac taaaaaagga cgccattatc
     9481 ttatgaagat tcttaacaag gagtgattct gtgaagagat gtattgtagt agtggaatag
     9541 tcaaaaacat aatatatatt aaaggagaga tttaaatatg actattccaa gttcaaataa
     9601 ggtatatcca agaagtaatg attatcaaac tgtatatctt aaaaatgtaa taacaaggga
     9661 aaatatagaa gttggagatt acacaatata taatgacttt tataatgacc caagagattt
     9721 tgagaaaaac aatgtattgt atcattaccc tataaacaat gataaattaa taattggtaa
     9781 gttttgctca atagcatgta atgcaaaatt tctaatgact agtggaaatc actcaatgaa
     9841 atcaaaatcc acatatactt ttcccatctt ttatgaagaa tggaaactcg atatcaacca
     9901 tataacggac gcttgggaca ataagggtaa cattataatt ggaaatgatg tgtggatagg
     9961 ttatgattct ataattatgt caggtgtaaa tattggtgat ggtgcaatta ttggaacaag
    10021 agcagtagtt actaatgacg ttccaccgta ttctattgtt ggaggagtac ctgcaaaaat
    10081 tataaaaaag agatttaata atgatacaat ttcaaaatta ttagaaatta aatggtggga
    10141 ttggccatat aaaaaaattc aatcaaacat taagcatatt caatcaggaa atattgataa
    10201 attagtttaa tattttataa tttatttata ttaaacttta ctttaataat cattttataa
    10261 gagctttttc aaaatttgta tattatttta atatatataa atttaataca acattaaata
    10321 aaataaaaag taaagttact taataaaaag ttactttact ttttattatt taaattcctg
    10381 tttttattat aataggtaaa attctattaa actagttcac attttcatta ttattgatat
    10441 attatatatg aatataccat tttgtttttt aataaaaaaa tcatgctaca tcaatagtaa
    10501 gcaactatta atgtagcatg attttttgta tctaaataat cttagtattt acctatcaat
    10561 atagattgat atatcaaaaa ctatagtctt ctatattctt ctctttttag cttttggtta
    10621 cttagatagt aatcaatttc atcaattaga cgttgtttat catcaggtaa tacacctttt
    10681 aaattagcat aatttgatgc atgattactt ctaaatacaa ttttttcatt agcatctata
    10741 ttctttatca tctctttaat ttcttgtaac acagccttat cctctagtat tgtaaactct
    10801 cctgccttaa gtaaatcgta aagttcagta ttaggttcta ccattaaact aagtacaccc
    10861 acataatctg gtgaaattgc tcctaacatt tttcctgtat caactgcatg ctgatgtgtt
    10921 tttttagttc ctccaagacc tgctattaca gtggctgata atttaatccc acttttttta
    10981 acttttctac caacttttat taattgttca ctactaaaac ctttcttaat atcttctaat
    11041 acttcattgc ttccactttc aagccctaga taaatcattt taacaccaag ttttcttaat
    11101 tctttaagtt catcatcaga ttttttctct atagatttag gagagccgta catagtaatt
    11161 ctttcacact ctggaaaaac ttcttttata tataagataa tatctcttaa tttctccatt
    11221 ggtattatca atgcatctcc atctgctaag aatattcttt ctacatgctt ataatatctt
    11281 ctaaatatat caatttcatt ttttattact tctagtggct ttattttaaa ttttttagat
    11341 ttgtacatac tacaaaacgt acatttgtta tgagaacatc ctagtgtgac ttgtattata
    11401 agactataag cctcacttgg tggtctgtaa agtggcatat cgtattccat attaaaatcc
    11461 cccttgtaat aaaatattca ttctattata ttattaatat ttactttaaa taataactta
    11521 taaaattata ttttttaaat tttagtttaa gatatattct ttaaaaattt aatgatacta
    11581 ttggcagtaa tattaagttg tttttcttga cttataatag catcattatc tcccttttgt
    11641 tttccataat ctccaaattg tgcatgattt cctccttcaa tctcaacata agttgtatca
    11701 tcaggaagtt tttgttttga ttctacaaga cttttaaaat tgactactcc atctttgctc
    11761 ccccatatag atataacttt ttttcctaat tctttcaact catcacccat tggataagaa
    11821 gctaaaaata caacgccttc aattaattta ttatcacgtg caaaatttga tgctgcaact
    11881 cctcctaatg aatgacctcc tattacccaa tgttctatgt tgttgtaaga atccataact
    11941 ttctgtgcct tattagttcc aagtatagct agattaagtg gcatctttac gattacaacc
    12001 tcatatccac tttctgctat ttttcttgca agtggagcat aagattctat ttctacttta
    12061 gctcctggat acaaaattaa acctttagaa acttttcttc cttttggtgt aaatgaaatg
    12121 ttttcattta cagttacttc aactttacta ttgctgacta gagcttcttt tgccagcttt
    12181 tgaggttcat atgttttatt tatccatatg aaaaagccaa gcaaaatcaa tacaaatagt
    12241 atacctattc ccttaaatat cttattcttt atgttaaatt ttctattatt atttttatca
    12301 tctttgttct caacctcatc ataaagtaaa ggtctctcaa gttccatagt tatactaaat
    12361 gtatcatatt tcttattacg ctttaccttt acatctttac tcataaatct aacccccata
    12421 atatttgaat actattatag tttatcctaa gtaaattgtt ttttcaacca atgaaaaaaa
    12481 tcaatatttt aactattttc atataaaatt cataaatata tgttttttgt tttttatata
    12541 ttattttctt ttagtatttt ataaaacaaa tagccatata ctttatttta aatatatggc
    12601 tattctataa tttaatatct aagctgcttc ttttattgaa actttagtta tattacttat
    12661 tctatacatt tctttgacac catttagagt ttgcagtaag tttagacata ttatcttgtc
    12721 acctagactt ttagtattta caatactctc taaaattaca cttcttcctt catcatactt
    12781 atctacattg taatttgaaa tataattggt atctctcaat attttttcaa catcttcatc
    12841 ttttatttta tttttattaa catttatata tagattatac atattactta ttagtaattt
    12901 ctgattttct aaaaaatcct cttgatgttt atctttatat atagcattta tcaacataag
    12961 tctatcttca ataagtgatg gtatcaataa taaatttttt atatagtaaa catcagctac
    13021 attttctata gacttattga cttcatcaat tatattttta actattcctt tatacatttc
    13081 tataacatgt tgatagcctg ttttagcatc atatggtaat ataaacttat ttactattaa
    13141 tgcaataatt gctcctaatg ctacatatga aatcctgctt attgttaaaa catttgggtc
    13201 tcccataact actgctgaac caagagctga aacagttaca cataccatta attttctata
    13261 atcaactaca taagagttta tataccctgc taaaagaact attaacgacc ttaatgtact
    13321 atcttttatt attgaaaata atacaataaa tattacagct cctataaatg taccttctat
    13381 tctttttcta gacctttgta tacaattttc tgcgtaaggt tgtgttaatg aaaagacagt
    13441 taacattatc catcttccat ctcttaaatg gaaataatcc ataataaaac ccgctacagc
    13501 tgtagctaaa gcaatcttta ctgcataaga aaatcttata gaatttaaat taaagttttt
    13561 cttatatatt gatagtcgtt taaattcatg aggtatcttt gattttcttt cagctttctt
    13621 ttcgtctctt gattttactt ttttataatc tactaaaaac tcatataaat tttctaaaac
    13681 actataaatt tcttttaaat ttatattttt agtatcctga gcaataacat aatctctaaa
    13741 tcctaatatc tctttatcag ttatggtatc tttattttct aaacacatct taatatagtt
    13801 tatttctttc gttgctacaa ctaaaacatc tgatttacta tttatgctac tttcttctaa
    13861 aatatttttt atatcttcat cttcaaattc tttactatcc tttttgtatc tttccaggat
    13921 taaacttatt ctttctaatg aaaataaaat attcattatg tctatacctc taatactgat
    13981 ataaaagtca tcttttc
//
LOCUS       DAEMGG010000287         4022 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.guided.210, whole genome shotgun sequence.
ACCESSION   DAEMGG010000287 DAEMGG010000000
VERSION     DAEMGG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.guided.210"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            18..782
                     /locus_tag="KPK24_003859"
     CDS             18..782
                     /locus_tag="KPK24_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2301448.1"
                     /translation="MTNTILLTKDKVYDGNLILVNAFLPVKTSEDIDLIPVDTRFPSI
                     LMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLRDNGKDFTKKFVALP
                     NHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYGFIERYQCKKEEITG
                     ISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKIEDKEKIIEIFYVPI
                     HTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            800..2938
                     /gene="vanT"
                     /locus_tag="KPK24_003860"
     CDS             800..2938
                     /gene="vanT"
                     /locus_tag="KPK24_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG2301449.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            3252..3872
                     /locus_tag="KPK24_003861"
     CDS             3252..3872
                     /locus_tag="KPK24_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG2301450.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
BASE COUNT         1452 a          456 c          667 g         1447 t
ORIGIN      
        1 aaaataaaga agataaaatg acaaatacta tcttattgac taaagataaa gtttatgatg
       61 ggaatctaat tctagtaaat gcttttttac cagttaaaac atctgaagat atagatttaa
      121 ttcctgtaga tacaaggttt ccaagtattc ttatgaaacg tgaagctaca aacattttac
      181 aaaatatttt aaaaagtata tgtggaataa atgaaatagt acctgtaagt ggatatcgta
      241 ctgcagagga acaacaagat atatattcaa gttctctgcg tgacaatgga aaagacttta
      301 ctaaaaagtt tgtagcactt ccaaatcaca gtgaacatca gacagggctt gctattgatt
      361 tagggttaaa gaaagaagtt atagatttta tctgccctga atttccttat aatggtattt
      421 gtaatgagtt tcgtaaggca gcactacatt atggttttat agagcgttat cagtgtaaaa
      481 aagaagaaat aacaggaata tctcaagaac catggcattt tcgttatgtg ggatatccgc
      541 attctgagat tatcaataaa atgaatctcg ctcttgaaga atatatagaa tatattaaga
      601 gattttcgta tacaaattgt ttaaaaatag aagataaaga aaaaattata gaaatctttt
      661 atgtgcctat tcatactaaa gaagaagtga gaatctctat acctgaacaa tgtgtatatc
      721 aaatatcggg taacaataag gatggtttta ttgtgacatt ttggagaaat agaaaggact
      781 aagaaatgga ggaacaagaa tgattaggaa taaatcttat gcagggcttg attattttag
      841 gattatagct gcattactta taattgcaat tcatacatct ccattattat catatagtaa
      901 aatggctgac tttgtgttaa ctagtataat tgctcgtatt gctgtgccat tcttttttat
      961 gctatcaggc ttttttctaa tttcaacata taattataat tgtaataaac tgaaatcttt
     1021 tctaaaaaat gtattattta tttacgggat agctattgta atttatattc ccttaaatat
     1081 ttacactgga tatttttcca tgaataattt agtgcccaat attattaagg acattgtatt
     1141 tgatgggact ttatatcatt tatggtatct acctgcatca attattggtg caagtattgc
     1201 atactttctt gtaaaaagac taggtttaaa gtactcacta gtaatcacta ttgttattta
     1261 tgtaattggt atgtttggtg atagttatta tggtttttta gaaaaaatac cactattaaa
     1321 gaatttatat gggtatattt ttgaagtatc agattataca cgaaatggta tattttttgc
     1381 acctgtattt ttcatacttg gtggaataat atctaataaa tctaattata tgtcaactaa
     1441 aaatagcttg attggatttc ttatatcatt cttacttatg ttatgtgagg gaatgttttt
     1501 acataaacta ggggtacagc gtcatgatag catgtatatt atgttattgc catgtatgta
     1561 tttcttattt acagccttaa catttttgaa aggttctcga gctaggttat tgcgaacatc
     1621 atcattaatt atttatatta tacatcctat gatgattgtt gttattcgta tgttttctaa
     1681 aatattcgga atacaaactg tcttggttga caatagcctt attcattata tagtggtaag
     1741 tatagcatct tttatagctt ctataattat tatattgatt cttaaacctc taaaaaaaga
     1801 cttgaaagga gaacataagg taaataccaa tagagcttgg attgaaattg atttaaataa
     1861 tttggaacat aatgtaaaga tgatacaaaa agctatgcca gaagattgtg aattgatggc
     1921 agttgtaaaa gctaatgcct atggtcatag tgcttttgaa gtagcgactt gtgcaaacca
     1981 aataggtgta aaagcatttg cagtagcaac aatagatgag gggattgatt tacgtcgata
     2041 tggaatacta ggagaaatat tgattcttgg atatactaac ccaataaggg caaaggaact
     2101 tcataaatac aatcttacac agagtgttat tgaccttaat catgctatct ctttaaacaa
     2161 acagaaatac aacataaaat cacacattaa aattgataca ggtatgcaca gactgggttt
     2221 tgatgttaat gatgtgaggt caattttaaa ggcatttaaa ttaaaatact tagatatatg
     2281 tggcatattt acacacttgt gtgtttcaga tagtctgaaa gatgaagatg tagactttac
     2341 aaataaacaa attgaatgtt tttataagtt aattgatttt ctgttaaaaa aaggtataaa
     2401 aattcctaaa gtacatattc aaagtagtta tggttttttt aattatccca atcttaagtg
     2461 taattatgta agaattggaa ttgcattata cgggatatta agttctccaa atgatacgac
     2521 taaattgcaa cttgatttac gacctgtttt atccttaaaa tcacaagtaa ttcttattag
     2581 aaaaatacaa aaagatgata gttttggata tggaagagtc tttattgcaa gcagagatag
     2641 cgtaattgct atattatcta ttggctatgc cgatggtata cctagaaatt tatcctgtgg
     2701 aaaaagttac gtaataataa atggttgtag agcagcaata gtgggtcgaa tttgcatgga
     2761 ccaacttgct gttgatatta cagatatacc taatgtagaa gttggtaata cggcaataat
     2821 aataggaaga gataatttat ctgaattatc tgcttctgaa gttgcaaata actcatgtag
     2881 tatatcgaat gaattattga gcagagtagg aagaaggtta aatgttataa agaagtaact
     2941 caattaaata gtaatatttg ttgtactgtg taatttattt tactctgact agacattttt
     3001 agtaatatta tgtctaatat atatttttta taacttacat gacaatttaa gttataaaaa
     3061 ggtgatgttt cagattttta ttttgaaaca tcaccttttt tgttataata tataataaaa
     3121 taattatacc ttaactgtaa acttatatag attatttact ttttaatatt agattcattt
     3181 tttgagattg gtatataata aacatataga gtatataata atagttaaag ttaaaaaagg
     3241 ggaaaagcaa tatgcagtat atacaagaac tacttgattt gactcaaaat tattgggtgt
     3301 tagccacaat aatcggctta ttaagtgcat ttatagaaag ctttatacca gccttaccac
     3361 ttgttgctat agttacagca aatgctgcaa tacaaggatt atttattgga tgtttgttat
     3421 catggattgg ctcaggactt gggacaactt cattattctt attaataagc agatttaatg
     3481 atagtaagct ttttaataag ttgagaaata gtaaaactga aaaagcaata agttggatgg
     3541 ataaacaggg atttaagtta ttatttatag cgtatgcctg tccatttatg cccggttgtt
     3601 tagttactat cgcatcagct tttggtaaaa aagatttaaa agattttgtg ccagcaatgt
     3661 tggcaggaaa atttgtaatg tttatagtta taagttacgt ggcaagtgat atagaaggtt
     3721 ttataacaag tcctttaaag atagcatcat ttatattatt ggtgtttttg tcatggaaga
     3781 ttggaaatag agtaaataaa aatcttgaaa atcataatta tgatttccat cataaaaaac
     3841 atcacaatga taaagatgat aaaatgattt agcttgtacg aaataaaacg tatcttaaaa
     3901 tgatttagta ctaaattatt ttaagatacg ttttattttt tgtattaagg tacaaaattt
     3961 ttagttcttt tgtaaactaa aatatataaa acttatttat acttagttca aaatattgtt
     4021 at
//
LOCUS       DAEMGG010000288         3946 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.guided.213, whole genome shotgun sequence.
ACCESSION   DAEMGG010000288 DAEMGG010000000
VERSION     DAEMGG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.guided.213"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1275
                     /locus_tag="KPK24_003862"
     CDS             <1..1275
                     /locus_tag="KPK24_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2301451.1"
                     /translation="EVFEGDSAPSVCPVCGAKEDQFIEVEEDEVTFRKDTDEYFVIVG
                     NGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEELGSDFYMEDKDWYD
                     KNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIPPIKGHDLENVFTL
                     RNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVVEAMDSILSKQLDK
                     EGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDCDMVVFSIGVRANT
                     QMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWPSSVEMGKIAGANA
                     SGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRIYKKLFMKDGSLVG
                     AILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            1709..2410
                     /locus_tag="KPK24_003863"
     CDS             1709..2410
                     /locus_tag="KPK24_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG2301452.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            2460..3542
                     /locus_tag="KPK24_003864"
     CDS             2460..3542
                     /locus_tag="KPK24_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2301453.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            3803..>3946
                     /locus_tag="KPK24_003865"
     CDS             3803..>3946
                     /locus_tag="KPK24_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HBG2301454.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGN"
BASE COUNT         1504 a          391 c          743 g         1308 t
ORIGIN      
        1 gaagtttttg aaggtgatag tgctcctagt gtatgccctg tatgtggagc taaggaagac
       61 caatttatag aagttgaaga agatgaagtt acttttagaa aagatactga tgaatatttc
      121 gtaatagtag gaaatggagc agctggtttt tatgcagcag atgctataag aaaaagaaat
      181 aaaacttgta agataactat gatttctaat gaagatgagt tgacttacta tagacctgca
      241 ctttctgatg gtataaatga agaacttggt tcagatttct acatggaaga taaagattgg
      301 tatgataaaa ataatatagt agtaatatta ggaacgaatg tagataaatt agatgaagtg
      361 aataaaacta ttatagtgaa tgatggagct attaaatttg ataagcttgt aatagctaca
      421 ggaagtagaa actttatacc accaattaag ggccatgatt tagaaaatgt gtttactctt
      481 agaaatataa aagatttata tagtgtaaaa gaagcactag aaaaatctaa gaaagttgta
      541 gtgattggtg gcggtttatt gggacttgaa gctgcatggg aatttagact aaaaggatta
      601 gaagtagtag tagttgaagc catggatagc atactatcaa aacaacttga taaagaaggt
      661 agtaaaatat tagaacaatg tgtaagagat acaggaatag atgtaagatt gggtgtagca
      721 gttgatggta ttgaaggtga tggaaaagct caaaaagttg tatttaaaga tggagatagt
      781 gtcgactgtg atatggtagt attttcaatt ggtgtaagag caaacactca aatggttcag
      841 gatacatctg taaaaattga tagaggtatt gttgtagata agacattaca gacaaatgtt
      901 aaagatatat atgcttgtgg agatgttgca caagtgggaa atattagttt agcaatatgg
      961 ccatcttcag tagaaatggg aaaaatagca ggggcaaatg caagtggaga taatttgaca
     1021 tttgaaagtg aagtatatcc agtttcatta gatgcaatga atgtaaaagt atttagtatt
     1081 ggtaatatac aaaattttga taaagaaatt tcatcaaaag atgaaggtca aagaatatat
     1141 aaaaaattat ttatgaaaga tggttcatta gttggagcta tacttataaa tgatttaagc
     1201 tgtactgtta aattgataag attaatatct gaaaaaggtg attttgagga tattatgaag
     1261 gctgatatac tataatttaa atacattaaa ataaaaagga gttgtctcaa aattgatttt
     1321 tttctaatca aattttaaat tcaactcctt ttttgaattt ttatataaaa taacatggaa
     1381 attttagtaa tgtctgtggt tacatacata ggtttaattg ttttttatat ggattgtctc
     1441 tggtataata aattgaattg tgtttgactg atagatattt ttattgaaag ttaatacctg
     1501 ttatgcgata ataaggacgt gttaggggag tttttataat aacaattaat ctcagttttt
     1561 gtgttccaat agtagcatca acacattctg atgtagtagt tcttttctta taaaaattag
     1621 ttacaaatat gcttgtaatt atttgatatg taaatttacc aatattataa atgatgacct
     1681 cgataataag gagagtgaaa aataatctat gaacgaaaat attttgattg tggatgatga
     1741 aaaagaaata gcagatttaa ttgaagtata tttaaaaaat gaaggtttta cagtatacaa
     1801 gttttataca ggtaaggaag ctctagaatg tattgaatct aaaaatttgg atatggctat
     1861 tttagatatt atgcttcctg atattgatgg atttcatatt tgccagaaaa ttcgagaaaa
     1921 atactattat cctatcatta tgttaacagc taaaattgaa gatatagaca agataatggg
     1981 tttaaccata ggtgctgatg attatataac aaagccattt aaccctttgg aagttgttgc
     2041 aagagtaaag acacaattaa gaagatatat gcgatataat aattcttatg aacaacagtc
     2101 gataattgtt aatgagtatg atattaaagg attgattatc aataaagaaa cacataaatg
     2161 tagtttattt gggaaagaag tagcattaac accgattgaa ttttctattc tttggtattt
     2221 atgtgaacat caaggaaaag tggttccttc tgaagaattg tttgaagctg tatggggaga
     2281 aaaatatctt gataataata acacagttat ggctcatata gggcgattac gtgaaaaaat
     2341 tcatgaacct tctaaaaacc cgaaatttat aaaaactgta tggggagtag gttatacaat
     2401 tgaaaagtaa aaatattaaa gtcaatcctg actttactca agtgagaaat aaaattttta
     2461 tgagaacagc tttaatggca tttatagcca ttattttcat atggatttta tattctttta
     2521 tattgtatgg taagttttct atctggatag tatcattctt tgaaaatgct tttcgtatgg
     2581 aatataaagc tgcattgaat ttatatcagc agattttccg taatagcatg gagctcttca
     2641 tgatactagc tatggtgatt gtgtttttta taatattccg ggtttattta aattggttta
     2701 caaaatattt tagtgaaata aatcaaggta ttgactctct tatcaaagaa gatgttggag
     2761 aagtggcttt atctccagag ttattggcaa tagaaaagaa aataaactca attaaacata
     2821 tacttgaaca acgtaaattt gagacacaaa tggcagaaca acgtaagaat gaacttattg
     2881 tttatcttgc acatgattta aaaacaccac ttacatcagt tatagggtat ctgacactac
     2941 ttcgagatga aagtcagatt tctgaagaac tgcgaaaaaa atatttatct atttcgttgg
     3001 ataaggcaga gcgtcttgaa gatttaatta atgaattctt tgaaattaca aggtttaatt
     3061 tatcaaatat atcacttgaa tacagtacag taagcttaac tcgtatgctt gaacaactaa
     3121 catatgagtt taaacctact ttgatgagta agaatttaaa atatacactg aatgttacac
     3181 caaatatgat gataaggtgt gatgttaata aaatgcaacg tgtatttgat aacttattac
     3241 gaaatgctgt ttattatagt tttgaagata ctactattga aataacagct gtacaggagg
     3301 atgattatat aaagcttaaa tttataaatc atagcaatac gattccagag gaaaaacttg
     3361 agcgtatttt tgaacaattt tacaggttgg atacatctcg taattcaaac agtggtagag
     3421 ctgggttggg acttgctata gctaaagaaa tagtggagct tcataacggg acaataacag
     3481 ctcatagcga gaacaatata attatcttta aggttattat tccgattgta ggaaatttgt
     3541 aagaatttaa gtataaaaaa ataggaaatt ctttaggata aataaataaa gagcactctt
     3601 gttattgata taattactgt atcaaaacaa gagtgctttt attttgatac agtaattata
     3661 tcaatagaaa aatgtgaaaa tatattatgt ggaacagatg caattgtaag tgaaggttga
     3721 ttggatttga aatagaaaaa gtattttaac atgtttctgt agattgtata aaaataaata
     3781 aatcaacaaa ggagaatcaa agatgaataa taaaaaaatt gcagttatat ttggtggaaa
     3841 ctcaacagaa tatgatgtat cattacaatc agcttttgca gtacttgaga atttgaatac
     3901 ggaaaaatat gatattatac caattggaat tacaagggag ggtaat
//
LOCUS       DAEMGG010000289         1570 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.guided.258, whole genome shotgun sequence.
ACCESSION   DAEMGG010000289 DAEMGG010000000
VERSION     DAEMGG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.guided.258"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            179..895
                     /locus_tag="KPK24_003866"
     CDS             179..895
                     /locus_tag="KPK24_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2301455.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            870..>1570
                     /locus_tag="KPK24_003867"
     CDS             870..>1570
                     /locus_tag="KPK24_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2301456.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAR"
BASE COUNT          695 a          136 c          282 g          457 t
ORIGIN      
        1 atataaaaca aaatgaaaaa atattaaatt taaactagaa ggaggtttct aagatggaca
       61 ttaaatacaa ataaccagaa caaaagatat aaatatatat ataaatggaa aaacttctat
      121 catattgtat aatcatatat gtaaagaaaa tttataaatt aatataaagg agattagaat
      181 gaattcatat aatattttgg tggtagaaga tgaaaaggag atagcagatg ctatagaaat
      241 atatctatta aatcaaggat ataatgtttt taagggatac aatggattag aaggtcttaa
      301 ggttatagaa aaccaagaaa tacatttagc tatagttgat ataatgatgc ctaaaatgga
      361 tggtataaca cttacaatga agcttagaga aaactataat tttccagtaa taatgctatc
      421 tgcaaaatca gaagaagttg acaaaataat ggggcttaat ataggagcag acgattatgt
      481 aacaaaacct tttaagccat tagaactatt ggctagagtt aattcacaac ttagaaggta
      541 cactaagtat ctaaacatgg ttggaaataa agagcaaaag gtagaaagtg acgatagtgt
      601 gtttgcaata ggcggattag aattaaatga aaatacaaaa gaagttagta ttgatggaaa
      661 gtatataaaa acaactccta tagaatttaa aatactaagt ttacttatga gaaatgcagg
      721 aagagttttt tctgctgatg aaatatatga aagagtttgg aatgagaatg ctgtaaatac
      781 agatacagtt atggtacatg tgaggaatat aagggaaaag atagaaattg acccaaaaaa
      841 tcctaaatat ttaaaggtgg tgtggggtgt tggatacaaa attgaaaaaa tttaaagata
      901 aatatatagt tactattata gtttttatac ttatgttatc agcatcatta ggtatgatta
      961 gtcaatattc tactatacaa gcaagtgcta aaggtggagc agagaatcca tttgaacagg
     1021 aaagctttgt agatagtata tataaaggaa gttatgtttt atatcataat atgaaggaag
     1081 aacaagaagg taagatgata caaccatcaa agctatttat caatgaagaa actatgaact
     1141 atataaaaaa tgtaagtaaa gaaaaagata cttatgatgg ttcagaaact tattatacag
     1201 atgatagtat taatgcagat tttaatgaaa actttgaaaa ttgggagcat ttcattaaag
     1261 cgtcaggaaa gaatttaaag tactatttag ttgataaaga aagcaatttt gaatttttta
     1321 atgataataa agaattagga gtacttgata caacaaaaaa tgatgcttca aaagaaaatt
     1381 ctaatgaaaa tcagtcaaaa aatacagaaa tgaatgatga ggaaagacaa aagcagatta
     1441 cagatataaa agaaaactat agattctacg ttgtaatgaa ttttgataaa gatggaaaag
     1501 taaatattgt ggattcttat ggagtaaatc aaaaaattat taatcaaatg ttgttatcta
     1561 aagcaagaga
//
LOCUS       DAEMGG010000290         1565 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.guided.259, whole genome shotgun sequence.
ACCESSION   DAEMGG010000290 DAEMGG010000000
VERSION     DAEMGG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.guided.259"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="KPK24_003868"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="KPK24_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG2301457.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          662 a          126 c          249 g          528 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaatga taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtattacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaaggc gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atataaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggaga
//
LOCUS       DAEMGG010000291          980 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2016019891
            SAMN15907406-rid12310173.guided.275, whole genome shotgun sequence.
ACCESSION   DAEMGG010000291 DAEMGG010000000
VERSION     DAEMGG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907406
            Sequence Read Archive: SRR12520702
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050554.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050554.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:48:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,788
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907406-rid12310173.guided.275"
                     /isolate="M2016019891"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: New York, Monroe County"
                     /collection_date="2016-10-29"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..956
                     /locus_tag="KPK24_003869"
     CDS             <1..956
                     /locus_tag="KPK24_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG2301458.1"
                     /translation="YHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDA
                     VSQNHIPNSAIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLS
                     SALCMDKDKSHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFG
                     ISKIYKKEELQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKG
                     FFDYTEKYTLKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEI
                     IFNEVNTIPGFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
BASE COUNT          354 a          107 c          188 g          331 t
ORIGIN      
        1 ggtatcatta tattggagat tataataata tccagaataa cacttggttt gaaagggata
       61 ctgatataat tccagttatt gtttcgcaga accgtgtcaa aaatggtatt attgaatttt
      121 taattcctga tgctgtttct cagaatcata ttccaaatag tgctattgaa tcatgtgaaa
      181 atgatattaa atacactaaa cttgattttg tgtttcctgt actgcatgga aaaaatggag
      241 aagatggaac agtgcagggg ttatttgaat tggcaggtat acaggttata ggatgtggta
      301 ctctttcttc agcattatgt atggacaaag acaaatcaca taaacttgtt cattcagaag
      361 gtattaaagt accaaaatca atattactaa aatactcaga taaatttaat gtggctgaac
      421 tgtcaaaaga cttaacttat ccattattta taaaacctgt gcgtgcaggt tcatcatttg
      481 gtattagtaa aatttataaa aaagaagagt tacaagtagc agtagacttg gcttttgaac
      541 atgataatga agtaataatt gaggaaaata ttgatggttt tgaggtaggg tgtgcagttt
      601 taggaaacga tgacttactt gttggaagag tagatgaaat agaattgtca aaaggatttt
      661 ttgattatac agaaaaatat acattgaaaa gttcaaaaat tcatatgcca gctagaattg
      721 atttggattg tgagaaaaga atacaagaga cagcaaaaat tatttataga actcttggat
      781 gttctggatt tgctagagta gatatgtttt taactccatc tggtgaaatt atttttaatg
      841 aagtcaatac aattcctggc tttacttctc atagtcgtta tccaaatatg atgaaaggta
      901 ttggattgtc ttttgaagaa atattggaca agataattga ggtgtacata aaatgatgaa
      961 taatatttta ttagataaaa
//
